LOCUS       DATMVF010000001        13376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_230108, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000001 DATMVF010000000
VERSION     DATMVF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230108"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VN178_00005"
     CDS             <1..592
                     /locus_tag="VN178_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HWS79371.1"
                     /translation="GPRNLAQICEGLGCDLLHVSTNYVFDGESGRAYEPFDTPNPISV
                     YGRTKLAGEEYVRQLTNRWYIVRSAGVYGKGHNFVRTMLRLGAQRNVLKVKDDESISP
                     TYAADLAQGILGAIEDRRYGLYHLTNSGSCSWHEFAKEIFSLAGVEVEVVPVSSSEYP
                     LPAARPANGVLSALGSPRLRPWREALEDYLEREKVI"
     gene            complement(585..2030)
                     /locus_tag="VN178_00010"
     CDS             complement(585..2030)
                     /locus_tag="VN178_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWS79372.1"
                     /translation="MSRVGRILLLSGACLALGFFLPGELEQGSIEAVGRPNIIFIVTD
                     DLDAASVSKVPSFSGYIAEKGVTFDNAFVTSALCCPSRATILTGQYPHNHLVRTNVPP
                     RGGFETFRDLGRERSTLATWLDEAGYETALFGKYLNGYGDAGRNHVPAGWDEWYGAVG
                     DAQLNQNGRLVRYRADTYLDDALSGLAQNFVRRQERRDAPFFVYLSVHAPHAPAKPAR
                     RHEGLYEGLRAPRTPSFDEADVSDKPEWVRDLELDSAVKKRIDELYSKRLKTMAAVGE
                     MIGGLLRTLEQTGKLDNTYVVLTSDNGYHMGQHRLGLGKQSAYEEDIRVPLMIRGPGL
                     PAGVSRDEMVLNNDFAPTFADLAGLPPPASVDGRSFASLLDRTRRNDPASWRTAFEIR
                     NWDNKRNEASYRSVAPVPPYRAVRTQRYLYVEYAAGERELYDLKKDPYELHNLYDWAD
                     KDLISKLGARLDALRDCAGTGCRAAEVGQPK"
     gene            complement(2055..3086)
                     /locus_tag="VN178_00015"
     CDS             complement(2055..3086)
                     /locus_tag="VN178_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79373.1"
                     /translation="MSRLGGTWRRNPPGEKADAARTRDDSTPVFFVTGIGKSGTRWLT
                     KILDSHPEVLCKGEGRFFSANWRRANLDPTNEKAVASSLYYALTHSEYLRLWIERSVW
                     SRDGNTDRHLNSLMRLATEYFLTEELIKSGKRLVGDKSPLLDEGFVREVGAVYPGARV
                     IHIIRDGRDRTVSSMHRGWRRASQGYVHRLSPEDLAKGKALREGQQERTEKGVFTEVW
                     LRQAARNWRLLVGRAIEDGPALLGPNYTEVRYEDLLEHPHAEVGRLLGFLGVDTDERL
                     VEHCVGSASFQRLSKGRRRGQEDPSSFYRKGIAGDWKNHFTEEDRRIFKEEAGELLIR
                     LGYEDDLDW"
     gene            complement(3179..4321)
                     /locus_tag="VN178_00020"
     CDS             complement(3179..4321)
                     /locus_tag="VN178_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWS79374.1"
                     /translation="MPIFEYRTTFEMARNIKAFLDLEVVLTRNGSRGTPKGRDAGTGG
                     SQRQVGGEAQNALRAGSTPDGSATRGATDEVVPMFFLVGRSKSGTSWLMRVFDSHPEI
                     LCRGEAKFFGKDNPNALHGALARSKHLKRWLDHNPWTLRDDDPNLDGIVANTISYLMR
                     EKLKKTGKKIVGDKSPFTTPGVVEEISSICPGAKVVHIVRDGRDVAVSSVHHQWNNAI
                     DQGGRRKLSREKVAKREAYRKAPEAFGASGESIFADEHVAEVARSWSTSVSRAMADAA
                     LLGDDYCQVRYEDLLAEPVGEVMRLLEFLGADSDVEVARGCVQAASFEQLSGGRTQGE
                     EASSSFYRKGIAGDWKNHFSEEDRRVFKEEAGELLIRLGYEDDFDW"
     gene            4508..5656
                     /locus_tag="VN178_00025"
     CDS             4508..5656
                     /locus_tag="VN178_00025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWS79375.1"
                     /translation="MKLSLCIATKNSAGYIEKLLRVGRSFADEVVVAVDASSNDRTER
                     ICHENADRVFSVESPGYVERILPWLNEQCRGDWILRLDDDELPSEGLIEILPSLMNDR
                     EMTHYHLRRRTMIGEDGTRWIAQRPWWPDWKLRLFRNIPSIVRIPGRLHSGYEVKGAS
                     RFLYRGSIYHYDFVYHDEQHRREKVRQYEIISPEKSGSHHYSLPDENSLVTRPIPADD
                     VPAQDHTESRPPAALAPIRHVDLAEIDLAAVQECDYGPALFRARLECIDCPRVMEAGR
                     WYKVDVELRNESPVAWPIPEMGAPDVRVTYHWLSADGELYDFKGRRTVLPHMLRPGET
                     ARLPAPVRSPKEPGDYILQWDMVIPQVAWFSGQGWRGPEIPVSVVRKA"
     gene            complement(5877..6944)
                     /locus_tag="VN178_00030"
     CDS             complement(5877..6944)
                     /locus_tag="VN178_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWS79376.1"
                     /translation="MPVFNSRTVIEIARGIRAFLNLEAGLTRGEIPARAPGVEDPPAF
                     ELARVRTRLEEKEREIAGLEQRLAARSSRAGGVDPENVVWIFCSNRSGSTWLSSMMGE
                     VEGHEVWNEPLVGKLFGDLYYGGAAGHQKVKHYILGDFHKGSWLDSVQSFVLSEATAR
                     FPGVAEGGYLIIKEPNGSIGAPLLSEAMPASRVIFLLRDPRDVAASGLDAARKGSWQY
                     ENAADRGWKREALADNQPDLWVRRRAQNYVRHAGKARDAYEAHKGPKVLIRYEELRDD
                     TRGTMERLYSTLGIEVGGEELARAVEKHAWENIPEKEKGQGKFYRKATPGGWREDLTP
                     GQVEIVEQVSAALLKEFYPGR"
     gene            complement(7001..8164)
                     /locus_tag="VN178_00035"
     CDS             complement(7001..8164)
                     /locus_tag="VN178_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWS79377.1"
                     /translation="MPILKKNIELRLWRNTSLFIDLETGVTGLEGLARGLGSRLGRVP
                     PLARAVGVPSARSKPARLERGSKTPVFFVTGVGKSGTSWLMRTLDSHPEILCKGEGRF
                     FAADWRRANFDPEGTQALASSLYYALLHSEYLRMWVERSVWAREGDAARHLDNLTRLA
                     TEHFLVGRLRKTNKKLVGDKSPLLDEDFIQEVSRIFPEAKVIHIIRDGRDQAVSMLHH
                     VWNRSTDQGGVQTLKPGEFERREAYRKDPEKLLQTGEGMFTEERLRGAARSWNARVGK
                     TSEDGPALLGPNYTEVRYEDLLERPNEEVSRLARFLGADTSEKAVHQAVDSASFERLS
                     KGRERGQEDTSSFYRKGVAGDWNNYFTERDKEIYKEEAGKLLIRLGYERDLDW"
     gene            complement(8234..9361)
                     /locus_tag="VN178_00040"
     CDS             complement(8234..9361)
                     /locus_tag="VN178_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79378.1"
                     /translation="MPVFSQRVALAVRNVKAFLKVEAGVVRGGVPESGGGTLGAAVRP
                     SGAGQDQRIEQLRRELREKDGENARLRSRLAAGSFHLPGGGVKPENMIWMFGSGRTGS
                     SWLSTMMADLEGHARWNEPYVGELFGSAYYLRAGDRMRGRKDYALGDDYREAWIRSIR
                     NFVLEGANARFSGLGENGYLAIKEPNGSIGAPLLAEAFPESRMILLVRDPRDVVSSAM
                     AAQRKGSWGEGWRANGGGDSLADTDPDEFARQWANMYTVSLGNARDAYEAHKGPKAVV
                     RYEDLRADTLRTMRRLYSTLDIQVGEQELARVVQMHAWENVPENKKGPDKPHRKATPG
                     GWKEDLTPEQARIVEELTVAVLDEFYPRWSTSYGRVKNLDP"
     gene            complement(9374..13021)
                     /locus_tag="VN178_00045"
     CDS             complement(9374..13021)
                     /locus_tag="VN178_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWS79379.1"
                     /translation="MPAMDICTIIAKNYIAHARVLAKSYKEHHPEGTCHVLVIDDTEG
                     YVDASSEPFELVTPAQLDIDDYEHMAAIYDVLELSTAVKPWLLRYLLDERSLERIAYL
                     DPDIRVYDRLDEIEGLTVEHGLVLIPHITTPLPRDGKRPSEADILISGTYNLGFISLE
                     RNPSTDALLDWWSERLKTDCVVAPDAGYFVDQRWMDFVHGVMPDFYVLRDPSYNVAYW
                     NLHGRDLTYRKGRYYVDGRPLHFFHFSGFDPDHRKRLSKHQTRIEIRRGSALARACNA
                     YADELLANGYRKAKNWPYTYGVLPNGVTLDSGMHRLYRKGEGAPRESIFTPQGADRFV
                     AWLNEPAPVGGEHGVTRYLYEIYSGRPDLRQEFPNLDGPDAMRLIEWHRIHGRVKLPY
                     PVHPSAEQDGHETPETSDPPVGVNVAGYFRAELGVGETARQVVAALERVGIPVATVGL
                     SASASRQEHAYETDAIRPTFPVNLICVNADMLPTFAERAGPGFFFDRYTIGLWWWETS
                     EFPDRFSRAFEPVDEVWVGSHFVADAISAVSPVPVVKMTMPVSMPEIEEFDRGDLGLP
                     EGFVFLFVFDYHSVFERKNPLALIEAFEQAFSEGSGASLVLKSINSEHYPEEHGRLME
                     AAKGRGDIHVIDRYVTAAEKNAMFAGCDCYVSLHRSEGFGNTLAEAMYLGKPVIATGY
                     SGNMEFMTQQNSYPVDYTMRAIGDAAGPYPATGEWAEPDVGHAARLMRRVFEDRGEAS
                     GRGKRAAEDLRRNHSAEAAGRAMAERIRRVRARRGAGPDALRPHVHSQSGLDTNPLTH
                     RIATTPQPATSGSTLGRALAPFRRVLLKLMWPFALQQRAVNEELLATTKEADQNLRAA
                     LNLIESRYAKDRADLLGAVNHNREQARTHGERLDRLERRLAEGAGGVPTPQTGSVGKQ
                     SDDPGPKAADTGGFSRQAISRLVTPFTAQQQKINDQLFAAVSTLGEKLGGLVPRVEAA
                     DRLVAETRALPYVSGSPFEIFEEPVAGRVQGYGRRKGDPGRGGTYDAFEDIFRGPEHF
                     IRDRQRRYLDILGDREPVLDIGCGRGEFLDLLRERGLEYAGTDPDPDMVGRCRAKGHE
                     RVEVADANSYLEKCEDDSIGAIFCAQVIEHMPYEELLRFYGLGLQKLRPGGLFILETV
                     NPHSVPALKTFWVDPTHQHPLFPEVALALCEISGFQSAYVFHPNGRGQVEVDRYETGE
                     YAVVAKKAAHE"
BASE COUNT         2223 a         4389 c         4131 g         2633 t
ORIGIN      
        1 cggtccgagg aacctggccc agatctgtga ggggctaggc tgcgatctcc tgcacgtcag
       61 cacgaactac gtcttcgacg gcgagtcggg acgagcctac gagcccttcg ataccccgaa
      121 ccccatcagc gtctacggcc gcaccaagct cgccggagag gagtacgtca ggcagcttac
      181 caaccgttgg tacatcgtcc gatccgcggg cgtctacggg aagggccaca acttcgtgcg
      241 gaccatgctg cgcctgggag cgcagaggaa cgtgttgaag gtcaaagacg acgaatccat
      301 ctcgccgacc tacgccgctg acctcgcgca ggggatcttg ggggcgatcg aagaccgacg
      361 ctacggcctc taccacctta ccaactctgg ctcctgctcc tggcacgagt ttgcgaagga
      421 gatcttctca ctcgccggcg tcgaggtcga ggtcgtcccc gtctccagct cggagtatcc
      481 tctccccgca gcacgacccg ccaacggcgt cctctccgcc ctggggagcc caagactgcg
      541 cccctggcgc gaggctctgg aagactacct cgagcgcgag aaggttattt aggctgcccg
      601 acctccgcgg cgcggcaacc tgtcccggcg cagtcgcgca gcgcgtcgag gcgcgcgccg
      661 agcttcgaga tgagatcctt gtccgcccaa tcgtacaggt tgtgcagctc gtaggggtct
      721 tttttcaggt cgtacagctc gcgctccccg gctgcgtact cgacgtacag gtatctctgt
      781 gtccttacgg cccggtaagg aggcacgggc gctaccgacc tataagacgc ttcgttcctc
      841 ttgttgtccc agttgcggat ctcgaaagcc gtgcgccagg aggcaggatc gttacgccgc
      901 gtcctgtcga gcagggatgc gaacgagcgg ccatcgacgg aagccggagg cggcaagcct
      961 gccaggtcag cgaaggtcgg ggcgaagtcg ttgttcaaca ccatctcgtc cctgctgacc
     1021 ccagcaggca atccgggtcc ccggatcatc aaaggtaccc ttatatcctc ttcgtaggcg
     1081 ctttgcttgc caagcccgag gcgatgctgg cccatgtgat agccgttgtc gctggtgaga
     1141 acaacgtagg tattgtctag cttcccggtc tgttcgagcg tccttagcaa cccgccgatc
     1201 atctcgccaa ccgcggccat cgtctttagc ctcttgctgt agagctcgtc gatacgtttc
     1261 ttcactgcgg agtcaagctc taggtcgcgc acccactctg gcttgtcgga gacgtctgct
     1321 tcgtcgaagg agggcgtacg gggcgcacgc aggccctcgt acagaccctc atgcctccgg
     1381 gcgggtttgg cgggcgcgtg gggggcgtga acggagagat acacgaaaaa tggggcgtcc
     1441 ctgcgttcct gacgtctcac aaagttctgg gcgagacccg aaagcgcatc atcgaggtag
     1501 gtgtccgccc ggtacctgac cagccgcccg ttctggttca gctgcgcgtc ccctacggcc
     1561 ccgtaccatt cgtcccaccc cgccggaacg tggttgcggc cagcgtcgcc gtagccgttg
     1621 aggtacttgc cgaacagggc cgtctcgtaa cccgcttcgt cgagccacgt ggccagcgtg
     1681 gatctctctc gtccgaggtc gcggaacgtt tcgaaacccc cgcgcggagg gacattggtc
     1741 ctgaccagat ggttgtgcgg gtactgtccg gtcaggatcg tggccctcga gggacaacaa
     1801 agcgcggagg tgacgaacgc gttgtcgaag gtaacgccct tctcggctat ataccccgag
     1861 aaggatggta ccttcgagac cgacgcggcg tccaggtcat ccgtgactat gaagatgatg
     1921 ttgggccggc ccactgcttc tatggagccc tgctctaact ctcccggcag gaagaaccct
     1981 agcgccaggc atgccccgga caacagcaag atcctgccta ccctactcat gacgtgggcc
     2041 gccacgacgc ggtgctacca gtcgaggtca tcctcgtaac cgagccggat caagagctcc
     2101 ccggcctcct ctttgaagat gcgcctgtcc tcctcggtga agtggttctt ccagtcaccc
     2161 gctatcccct tgcggtagaa agaggagggg tcctcctgac cccgccggcg ccccttggag
     2221 agcctctgga agctcgccga gcccacgcag tgctctacca gcctctcgtc ggtgtcgacc
     2281 cccaggaagc ccagcagccg acccacctcc gcgtgggggt gctccagcag gtcctcgtaa
     2341 cgtacctcgg tgtagtttgg cccgagcagg gccgggccgt cctcgatggc tctacccacc
     2401 agaagtctcc agttacgggc cgcctgccgc agccacacct cggtgaacac gcccttctcc
     2461 gtccgttcct gctggccctc acgcagggcc ttgcccttcg ccagatcctc gggcgagagc
     2521 ctgtggacgt agccctggct ggcgcggcgc cacccgcggt gcatggagga gaccgtcctg
     2581 tccctgccgt cccttataat gtggatgacc ctggctcccg gatacacggc acccacttcc
     2641 cgcacgaacc cttcgtccaa gagcggcgac ttgtctccga caagcctctt gccgctcttg
     2701 atgagctctt ccgtgaggaa atactccgtg gccaggcgca tgagggagtt gaggtgcctg
     2761 tcggtattcc catcccggct ccatacggag cgttcgatcc agagccttag gtattcggag
     2821 tgggtcaggg cgtagtagag ggagctcgcg acggccttct cgttcgtggg gtcgaggttc
     2881 gcccgcctcc agttcgcgga gaaaaaacgc ccctcaccct tacacagcac ctcagggtgg
     2941 gagtccagga tcttggtcag ccaccgggtc cctgatttgc cgattcccgt aacgaagaat
     3001 acgggcgtgc tatcgtctcg cgtcctcgca gcgtccgcct tctcgccagg gggattccgg
     3061 cgccacgttc ccccgagtct gctcacccta gcgtacgacg ctcctgacca gggcggtgcg
     3121 agttcccttg cctggtttgc tgtcatgtcg ttctccaaac gtgcgtccgg gtaccgtgct
     3181 accagtcgaa gtcgtcttcg taaccgagcc tgatcaagag ttccccggcc tcctctttga
     3241 agacgcgcct gtcctcctcc gaaaagtggt tcttccagtc gcccgctatc cccttgcggt
     3301 agaaagaaga cgaggcttcc tcgccctggg tcctgccgcc ggagagctgt tcgaagctcg
     3361 cggcctgtac gcacccgcgg gcgacctcca catcggagtc ggcccccagg aactccagca
     3421 gcctcatgac ctcaccgacc ggctcggcaa ggaggtcctc gtagcgtacc tggcagtaat
     3481 cgtcgccaag aagagcagca tcagccatcg ccctgctgac ggacgtactc cagctcctgg
     3541 ctacctccgc cacgtgttcg tcggcaaaga tgctctcccc tgaggcaccg aaggcctcgg
     3601 gggctttacg gtaggcttca cgctttgcaa ccttctcgcg cgagagcttg cggcggccgc
     3661 cctggtcgat cgcattattc cactggtggt gaaccgagga cacggccacg tccctgccgt
     3721 ccctgactat gtgcacgacc ttcgcaccgg gacagataga ggagatctcc tctaccacgc
     3781 cgggggtggt gaacgggctc ttgtccccga cgatcttctt gcccgtcttc ttgagcttct
     3841 ctcgcatgag gtagcttatg gtgttggcca cgatgccgtc gaggttggga tcatcatccc
     3901 ggagggtcca ggggttgtgg tccagccacc gcttcagatg cttcgaacgg gcgagggcgc
     3961 cgtggagggc gttggggttg tccttgccga agaacttggc ctcccccctg cagagtatct
     4021 ccggatggga gtcgaagacc ctcatcaacc aggaagtccc cgatttcgaa cgccctacga
     4081 ggaagaacat cggtacgacc tcgtccgtag cgccgcgcgt ggcgctcccg tccggagtgg
     4141 accccgcccg cagtgcgttt tgcgcttcgc cgcctacctg gcgctgggat ccccctgttc
     4201 ctgcatcgcg gccctttggc gtaccccgac tcccgttgcg ggtgaggact acctcgaggt
     4261 ccaggaacgc ctttatgttc cgagccatct cgaaggtcgt gcggtactcg aagatcggca
     4321 cccggtgttt ctcctatcga gctacgttgc tactacaaag tacgatgctc tcataataac
     4381 ccgattccta ctagtggtat cggagccgct tcgtcctcac acagagctgt atctccggca
     4441 catcttgata gactgtcacg catcgtcgtt gttcgtcgtc gggtcgagat gccgttggga
     4501 ggcgtgggtg aagctctcgt tgtgtatagc gacgaagaac tcggccgggt acatcgagaa
     4561 gctcttgcgg gtcgggagga gcttcgccga cgaagtcgtc gtggccgtcg acgcctcgag
     4621 caatgaccgg accgagagga tctgccacga gaacgccgac agggtcttta gcgtagagtc
     4681 gcccggctac gtggagcgga tcctcccctg gctcaacgag cagtgtcggg gggattggat
     4741 cctccgcctc gacgacgatg agctccccag cgaaggcctg atcgagatcc tgccttctct
     4801 catgaacgac cgcgagatga cccattacca tctcagacgg cgcacgatga tcggggaaga
     4861 tggaacgcgg tggatagccc agcgtccgtg gtggcccgac tggaagctga ggttgttccg
     4921 caacatccct tcgatcgtgc gcattcccgg tcgcctgcac tccggatacg aggtcaaggg
     4981 ggcctcgcgc tttctctaca ggggttcgat ctatcactac gacttcgtgt atcacgacga
     5041 gcagcatcgc agggagaagg tgcgccagta cgagatcata tcgcccgaga agagcggcag
     5101 tcatcactat tcccttcccg atgagaactc gcttgtcaca cgcccgatcc cggccgatga
     5161 cgtgcccgca caagaccata ccgaaagcag accgcccgcc gcgcttgccc cgatcaggca
     5221 cgtggacctg gccgagatcg acctggcggc cgttcaagag tgcgactacg gcccggccct
     5281 gttcagggcg cgtctcgagt gcatagattg cccacgagtc atggaagcgg gacggtggta
     5341 caaggtcgac gtcgaactgc gcaacgaaag tcctgtagcg tggcctatac ccgagatggg
     5401 agcgcctgac gtgcgcgtta cctaccactg gctcagtgca gatggagaac tctacgattt
     5461 caagggccgc cggacggtgc taccgcacat gctgaggccc ggcgagacag ccaggctgcc
     5521 agcgccggtg cgctccccca aagagcccgg agactacatc ctccagtggg acatggtcat
     5581 cccgcaggtc gcctggttct ccgggcaggg atggcgagga cccgagatcc cggttagcgt
     5641 cgtgcgtaag gcttgagagc cttacgcacg acgagcatct cagcacgctc agcccttcgg
     5701 gcttcgctcg tcagcacttc agcatttcag ctttgttggg agcggctggc ttaggttgtt
     5761 ctctctgcga tggatggggc ctgcatcgtt tgtcggggtg ccaggctgtc aggcacttgc
     5821 tgagctgcgg aaatgctgag agccgccgta ggcggcgtgc tgacttgctg aaatgcttat
     5881 cggcccgggt agaactcctt tagcaaagca gccgatacct gctccactat ctctacctgg
     5941 ccgggggtga gatcttccct ccagccgccc ggcgtcgcct tgcggtagaa ttttccctgt
     6001 cccttctctt tctcggggat gttctcccag gcgtgtttct cgacagcccg cgcgagttcc
     6061 tctcccccga cttcgatgcc cagcgtcgag tacaggcgct ccatggtgcc gcgagtatcg
     6121 tcgcgtagct cctcgtagcg gatcaggacc ttgggaccct tgtgggcctc gtaggcgtcc
     6181 ctggctttgc cggcgtggcg cacgtaattc tgggcccggc gcctcaccca gagatccggc
     6241 tggttatccg ccagcgcctc cctcttccag ccgcgatccg ccgcgttctc gtactgccag
     6301 ctgcccttcc tggcggcgtc caggcctgaa gcggcgacat ccctggggtc gcgcaggagg
     6361 aagatcaccc tgctcgccgg catcgcctcg gagagcagcg gcgcgcctat ggagccgttc
     6421 ggctccttta tgatcaggta accgccctcg gccaccccgg gaaagcgcgc ggtcgcttcg
     6481 ctgaggacga aggattggac ggaatcgagc cagctccctt tgtgaaagtc cccgagtatg
     6541 tagtgcttga ccttctggtg gcctgccgct ccgccatagt aaaggtcgcc gaagagcttc
     6601 ccgaccagcg gctcgttcca cacctcgtgg ccctcgacct cgcccatcat cgaactgagc
     6661 caggtgctgc cgctgcgatt ggagcagaag atccagacga cgttctcggg gtcgacgccg
     6721 cctgccctcg aagagcgcgc tgccagcctc tgttctagcc cggcgatctc tcgctctttc
     6781 tcttcgagcc gcgtgcggac ccgcgcaagc tcgaaagcgg gtggatcctc aacgcccggc
     6841 gctctcgccg gtatctcgcc gcgggtaagg ccggcctcca ggttcaagaa cgctcttatg
     6901 ccccgcgcta tctcgataac cgtgcggctg ttgaagactg gcatcagggg tccttcctgc
     6961 tacgaagcgt cgtttcggcg agcattgtgc tagatcctgc ctaccaatcc aggtctctct
     7021 cgtagccgag ccggatcagg agtttgcccg cctcttcttt gtagatctcc ttgtcgcgct
     7081 cggtgaaata gttgttccaa tcacccgcga ctcctttacg atagaacgat gacgtatcct
     7141 cctgcccccg ctcacgtcct ttggagagtc tctcgaagct cgccgagtct acggcttggt
     7201 gcacggcctt ctcgctggta tcggcgccga ggaatcttgc cagcctgctt acctcttcat
     7261 tggggcgctc gagcaggtct tcgtaacgta cctcggtgta gtttggcccg agcaaggccg
     7321 ggccgtcctc tgaggtcttg cccaccctcg cattccagct cctggccgcc ccgcgcagcc
     7381 gctcctcggt gaacatgccc tcgcccgtct gaaggagctt ttccgggtct ttgcggtagg
     7441 cttcgcgcct ctcgaactct ccgggcttca gggtctggac gccgccctgg tccgtggagc
     7501 ggttccaaac gtggtgcagc atggagacgg cctgatccct accgtccctt atgatgtgga
     7561 tgactttggc ctcggggaag atcctgctca cctcctggat gaaatcctca tcgagcagcg
     7621 gcgacttgtc gcccacaagc ttcttgttgg tcttgcggag tcgccccacg aggaaatgct
     7681 cggtcgcaag gcgggtcagg ttgtcgaggt gcctggcggc gtcgccttcc ctggcccaga
     7741 cggagcgctc gacccacatc ctcaggtact cggagtggag cagcgcgtag tacagggagc
     7801 tcgccagggc ctgcgtgccc tcggggtcga agttcgcccg cctccagtcg gcggcgaaaa
     7861 accgcccctc gcccttgcag agtatctcgg ggtgagagtc cagcgtcctc atcagccagg
     7921 aggtgcccga cttgcccacc cccgtaacga agaacacagg ggtcttggat cctcgttcca
     7981 ggcgcgcagg tttgcttctc gccgacggaa ccccgaccgc tctcgccagc gggggcaccc
     8041 tgccaaggcg cgacccgagt cctctagcca aaccctcgag gcccgtcaca cccgtctcca
     8101 ggtcgatgaa caggctggtg tttcgccata gcctgagctc tatattcttc ttcagaatag
     8161 gcatccatct ctccattcga agtcaaaggc ctggcggcgt tacgcccgcc cttcacccgc
     8221 tggttcgtcc tattcacgga tcgaggttct tcaccctccc gtaggaagtg ctccaccggg
     8281 ggtagaactc gtcgaggacg gcgaccgtca actcctctac tatcctggcc tgctcggggg
     8341 tgaggtcttc cttccagccc cccggggtcg ccttgcggtg gggtttgtcc ggccccttct
     8401 tgttctcggg tacgttctcc cacgcgtgca tctggactac cctggcgagc tcctgttcgc
     8461 ctacctggat atccagcgtc gagtagagtc gtctcatggt ccttagggtg tctgccctga
     8521 ggtcctcgta gcggacgacg gccttgggac ctttgtgcgc ctcgtaagca tctctggcgt
     8581 tgccgaggct caccgtgtac atgttggccc actgccgcgc gaactcgtca gggtcggtgt
     8641 cggcgagcga atcgccgccc ccgttagccc gccatccttc gccccagcta cccttcctct
     8701 gggctgccat ggccgaggag acaacgtccc tggggtcacg gaccagcagg atcatgcggc
     8761 tctccgggaa ggcttcggcc aagagcggcg ccccgatgga gccgttgggc tctttgatcg
     8821 ccaggtagcc gttctcgccg agccccgaga agcgcgcgtt cgcgccctcc agcacgaagt
     8881 tgcggatgga ccggatccag gcctccctgt aatcatcccc cagggcgtag tctttgcgcc
     8941 cccgcatcct gtctccggcc ctgaggtagt aagccgagcc gaagagctcg ccgacgtacg
     9001 gttcgttcca cctggcgtgg ccctcgagat ccgccatcat ggtgctcaac cagctgctcc
     9061 ccgtccgtcc cgagccgaac atccagatca tgttctccgg ctttacgcct ccgccgggca
     9121 gatggaaact ccccgcggcc agccgggagc gcagcctggc gttctccccg tccttctccc
     9181 tgagctctcg acgcagttgt tcgatcctct ggtcctgacc ggcgccggaa ggacgcactg
     9241 ccgcgccgag cgtaccacct cccgattcgg gtaccccgcc gcgaacgacc ccggcttcca
     9301 ccttcaagaa agcctttacg tttcggaccg ccaatgctac gcgctggctg aagaccggca
     9361 tcctaaggtc tcctcattcg tgggccgcct tcttcgcgac gaccgcgtac tcgcccgtct
     9421 cgtagcggtc gacctccacc tggcccctcc cgttggggtg gaacacgtag gcgctctgga
     9481 agccgcttat ctcgcagagc gccagcgcca cctcggggaa gagcgggtgc tggtgggtgg
     9541 ggtctaccca gaacgtcttc aacgcgggga cagagtgcgg gtttacggtc tccaggataa
     9601 agagtccccc tggccggagc ttctgcaagc caagaccgta gaagcgtagg agctcctcgt
     9661 agggcatgtg ctctatcacc tgggcgcaga agatggcgcc tatagaatcg tcctcgcact
     9721 tctccaggta ggagttcgcg tccgccacct ccacacgctc gtggcccttg gccctgcacc
     9781 gcccgaccat gtcggggtcg ggatctgtgc cggcgtactc caggccccgc tcgcgcagga
     9841 gatccagaaa ctccccacgt ccgcacccga tatccagcac gggctcgcgg tccccgagga
     9901 tgtcgagata gcgacgctgg cggtcgcgga taaagtgctc ggggccgcgg aagatgtcct
     9961 cgaacgcgtc gtaggtcccc ccacgcccgg gatccccctt gcggcggccg tagccctgga
    10021 cgcgcccggc caccggctcc tcgaagatct cgaacgggga gcccgacacg taagggagag
    10081 cgcgggtctc ggccaccagc cggtctgcgg cctccacgcg gggaacgaga ccgcccagct
    10141 tctcgccgag cgtgctgact gcggcgaaga gctggtcgtt gatcttctgc tgctgcgccg
    10201 taaagggggt aactagacgg gatatcgcct gcctggagaa gccgccagta tccgccgctt
    10261 tcgggccggg gtcgtccgat tgtttgccga cgctaccggt ctgcggcgtt ggtacgcctc
    10321 cggcgccctc ggccagccgt ctctccaggc ggtcgagccg ctcaccgtgc gtcctggcct
    10381 gctccctgtt gtggttaacg gcgcccagca aatcggcgcg gtctttcgcg taacgcgact
    10441 cgatcaggtt gagagcggcg cgcaggttct gatccgcctc cttcgtcgtg gcgaggagct
    10501 cttcgttgac cgcgcgctgt tgcagggcga agggccacat cagcttaagc aggacccgcc
    10561 gaaagggggc gagggccctg ccgagggtgg agcccgaagt cgccggctgc ggggtggtcg
    10621 cgatgcggtg tgtaagcggg ttggtgtcga gtcccgactg gctgtgcacg tgcggcctga
    10681 gggcgtcggg tcccgcgccg cgccgcgccc tgacacgccg gatgcgctcc gccatcgccc
    10741 tccccgcggc ctcggcggag tggtttcggc ggaggtcctc cgcagccctc ttgccgcgcc
    10801 cggatgcctc cccccgatcc tcgaagacgc ggcgcatcag gcgcgcggcg tgcccgacgt
    10861 cgggttcggc ccactcgccg gtggcggggt aaggcccggc ggcgtcgccg atggccctca
    10921 tcgtgtagtc gacggggtaa ctgttctgct gcgtcatgaa ctccatgttg cccgagtacc
    10981 cggttgcgat caccggcttg ccgaggtaca tggcctcggc gagggtgttg ccgaagccct
    11041 ccgaacggtg aagcgagacg tagcagtcgc agcccgcgaa catggcgttc ttctcggccg
    11101 ccgtgacgta gcggtctatg acgtggatat ccccgcggcc tttcgcggcc tccatcagcc
    11161 tcccgtgctc ttccgggtag tgctcgctgt tgatggactt gagcacgagg gaggcccccg
    11221 agccctcgga gaaggcttgc tcgaacgcct cgatgagggc gagcgggttc ttgcgctcga
    11281 agacgctgtg gtagtcgaag acgaaaagga agacgaagcc ctcggggagt ccgaggtccc
    11341 cgcggtcgaa ctcctctatc tccggcatgg acaccggcat cgtcatcttg acgacgggga
    11401 ccggcgagac ggccgagatc gcgtcggcca cgaagtgcga gccgacccag acctcgtcca
    11461 ccggctcgaa ggccctagag aagcgatctg ggaactcgct cgtctcccac caccacagcc
    11521 cgatggtgta acggtcgaag aagaaccccg gccctgccct ctcggcgaag gtcgggagca
    11581 tgtcggcgtt tacgcagatg aggttgaccg ggaaggtcgg cctgatcgcg tcggtctcgt
    11641 aagcgtgctc ctggcggctc gccgaggcgg agaggccgac cgtcgccacc ggtatcccta
    11701 ccctctccag cgccgcgacc acctgccttg cggtctcgcc gacgccgagc tcggcgcgga
    11761 agtacccggc gacgttgacc ccgacgggag gatcggaggt ctcgggcgtc tcgtggccgt
    11821 cttgctctgc ggaggggtgc acggggtaag gcagcttcac ccgcccgtgg atgcggtgcc
    11881 actcgatgag gcgcatcgcg tccgggccgt cgaggttggg gaactcctgc ctgaggtcgg
    11941 ggcggcccga gtagatctcg tagaggtaac gcgtaacgcc gtgctccccg cctacggggg
    12001 ccggctcgtt gagccaggcg acgaacctgt cggcgccctg tggcgtgaag atggattctc
    12061 gcggggcccc ctcgcccttg cggtagagcc tgtgcatacc cgagtcgagc gttacgccgt
    12121 tcggcagcac cccgtaggta tagggccagt tcttcgcctt tctgtacccg tttgctaaca
    12181 actcgtccgc gtaagcgtta caggcgcggg ccagcgcgga gccgcgccgg atctcgatgc
    12241 gggtttggtg tttgctgagc cgctttctgt ggtcgggatc gaagccggag aagtggaaga
    12301 aatgcagggg ccgaccgtcg acgtagtagc gccccttgcg gtaggtcaga tctctaccgt
    12361 gcaggttcca gtaggccacg ttgtaggagg gatcgcgcaa aacgtagaag tccggcatta
    12421 cgccatgcac gaagtccatc caccgctggt cgacgaagta gccggcatcg ggggcgacca
    12481 cgcagtccgt cttcaggcgc tcggaccacc agtcgagcag cgcgtccgta ctcgggtttc
    12541 gttccaggga gataaagccc aggttgtagg tgcccgagat caggatgtca gcctcggagg
    12601 gcctcttccc gtcgcggggc aggggggtgg tgatgtgggg gatgaggacg agcccgtgct
    12661 cgacggtcag cccctctatc tcgtccagcc tgtcgtagac gcggatatcc gggtcgagat
    12721 aagcgatccg ctccagagag cgctcgtcga gcaggtaccg cagcagccac ggcttcacgg
    12781 cggtcgagag ctcgagcacg tcatagatcg ccgccatgtg ctcgtagtcg tcgatatcta
    12841 gttgggccgg ggtcaccagc tcgaacggtt ccgaggaggc gtctacgtaa ccttctgtat
    12901 cgtctatgac gagcacgtgg cacgtaccct cggggtgatg ctccttgtag gacttcgcca
    12961 acaccctcgc gtgggcgata tagttcttgg cgatgatcgt gcagatatcc atagccggca
    13021 ttgaatcata ctcggcgtgg cacttcgcaa aatggccagg aacctgatac ggttcagcta
    13081 taaattagtg aaccttcaag gtgtagaatg atttcgtgga taccattgaa tagagcgcct
    13141 gacgattcga agacgcaaag tatgtggtcc ttcacgatct gagacgatac caagattctg
    13201 ggggagaaag gtgatcgaag cacgaagccg ggccaagtgg aacaacaggg aggggatatc
    13261 cgagtacgcg gtccgctgga actgggcgga ggactcctcg gactcgggat tcaccgcggt
    13321 cttgagggtc aaggacgagg cccgctcctt gcccttctcg ctgcccgggg tgctgc
//
LOCUS       DATMVF010000002         8477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_230355, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000002 DATMVF010000000
VERSION     DATMVF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230355"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="trmFO"
                     /locus_tag="VN178_00050"
     CDS             <1..843
                     /gene="trmFO"
                     /locus_tag="VN178_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HWS79380.1"
                     /translation="HRDSMDDSIIYRASRYGKGEAAYLNCPMTDEQYYAFVEDLAVAE
                     LSPIKDFEEDLYFEGCLPVETIARRGKETLRFGPMKPVGLPDPRTGEIPYAVVQLRQD
                     DAEGRLYNIVGFQTRLKWGEQRRVFRTIPGLQNADFARMGVMHRNTYLPSNHMLEATM
                     RIREALGEAPLFFAGQLTGVEGYVESTAMGHIAGTNAARIARGEEPLTLPRGTMMGAL
                     AHYITTKEGTLQPINSNWGLVPTVPKRENGRRLSKPERRQRQARMALSTLDEFMREGV
                     KTAV"
     gene            complement(910..1587)
                     /locus_tag="VN178_00055"
     CDS             complement(910..1587)
                     /locus_tag="VN178_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79381.1"
                     /translation="MAEGNPLRLGGLSGLLAVVSMVPAYLVGYPDAPGSLLEAQTYFD
                     AESDMFVFSNGVLPLFHVFFFLLFLGVLYGMLRSPGGETRAVAEGLPAVALAGGIVFA
                     TLSAAGFTVEIFYPAVLQRFGDLSSDTAFVLVSLTLSSWLYHYCQIGASAMVLSTSMV
                     AVGTRVLPSWLALAGFLVAILTFLHFLFPLVAALVGLAWVALVSAVMLTGGARRGAQR
                     FRTRRTR"
     gene            1750..1995
                     /locus_tag="VN178_00060"
     CDS             1750..1995
                     /locus_tag="VN178_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79382.1"
                     /translation="MSETRSEKEIPEAWVGQRVRVGHAHGASTHTDGVLLGVGDRGIV
                     VEGGELVRFYPWTSVGSIEKAHEGGTEDPGVQARRAW"
     gene            complement(2054..2794)
                     /locus_tag="VN178_00065"
     CDS             complement(2054..2794)
                     /locus_tag="VN178_00065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS79383.1"
                     /translation="MGSEHPKLYAELAGWWQLISPTEDYADEAAFFAKLLRAEGAGTV
                     LELGCGGGNVAWFLKKDFDLTLTDISGAMLAQSKKQNPELEHIEGDMRTLRLDRTFDG
                     ILIHDAIMYMSSEEDLRAALLTAHEHCRPGGVAVFVPDWVAETFQPGTTHEGVDRGDR
                     GVRYLEWTWDADPNDTKVNYEFMLALKDGDELRAVVDRQVVGVFPRATWLRLLEDVGF
                     EPEVVEDPSTGGERVEVFVGHKPLPPAG"
     gene            complement(2840..3796)
                     /locus_tag="VN178_00070"
     CDS             complement(2840..3796)
                     /locus_tag="VN178_00070"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWS79384.1"
                     /translation="MARVAVLRGGHSMERDVSFITGRRVQHALERLGHDVHPLDIEDS
                     TTGALMEISPDAAFICLHGPGGEDGTVQALLETLGIPYTGSGPLSSIRCMDKDYAKRA
                     LRAAGIQTPPFRTFLRRAMNEMGASAALDVAAEELGYPLVVKPAREGSSFGLSRVEGA
                     EGLFDAVYEALGYDAKILLEHWVEGTEISIPIIEPAGEEPRALGLVEIRPREGAYNFE
                     AKYTPGATDFAIPAEIPHEAATRVEETALTVYKTLGCSGFARVDTIFAGGTPWILDVK
                     TIPGFTETSTFPLAAETTGTTFETLVETILEAALRTEAAAKL"
     gene            complement(3803..5128)
                     /locus_tag="VN178_00075"
     CDS             complement(3803..5128)
                     /locus_tag="VN178_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWS79385.1"
                     /translation="MPLVDLDRFDFLYANRVKGMKTAATRDLMATLSRPGIISLAGGF
                     PDTRAFGEEAFREISRNIASDAAQALQYGPTAGLEAIKDVIVEVMGAEGTPARQEDVF
                     VTTGAQQGLDLIAKVFLDEGDAVLCEGPTYAGALNAFASYRPRIAHVPMDRAGIIPVA
                     ARETLKRARKGGLHVKFLYTVPNFQNPAGVTMVAERRRELLEMAREFDLVIVEDNPYG
                     MLRFEGEPLPSLAALEQEEGDLDRVVYLGTFSKIFAPGVRLGWVHAQPGILHKINVGK
                     QGADLCSSNLSQMMISSYFQNADWRAYVRRLTSMYKERRDAMLDSLAEFMPKEVYWTH
                     PEGGLFVWATLPSYLDATAMLPRAIARNVAYVPGEGFYGGAPGTGKNNMRLNFSFVEP
                     ERIRRGIELLSEVIRERMELRSDLERGSHRKESAIHGGRAAAFGSGTDG"
     gene            5270..5806
                     /locus_tag="VN178_00080"
     CDS             5270..5806
                     /locus_tag="VN178_00080"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HWS79386.1"
                     /translation="MTTNEHKRREVQEILGFELERADLDLPEIQAIEPAEVAAQKARA
                     AREALGRPGLPVLVEDSGLMVDAWDGFPGALTKWLMQSVGNEGLLRMLAPGEDRSARA
                     VCVVALAEAGGTVRTFRGEVPGTLAQRPRGTGGFGYDPVFVPGWSSMTYAEMGEGKNR
                     DSHRTRAFRAVREWLGGA"
     gene            5902..6966
                     /gene="pdhA"
                     /locus_tag="VN178_00085"
     CDS             5902..6966
                     /gene="pdhA"
                     /locus_tag="VN178_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWS79387.1"
                     /translation="MSADNEHSEDGGIETREDGGAIATSSERLVDLYRQMALIRAFEE
                     ACQRGFRQGKIGGYLHVYIGQEAVATGFLDAFKEGDRIITAYRDHAHALLLGSDPNEV
                     MAELYGRETGLVKGKGGSMHLFDVERGLMGGYGIVGGHIPLGVGVAYAMRYQETDHIC
                     QLYLGDGAMSQGAFHEAANLAGLWGKEGLCPCLFIIENNKYGMGTSVERTTAMTDLAA
                     KFDAYGIEHEKVDGMDVEAVVEVAERVAGQVRETGKPYAVEAITYRTAPHGAADFFEK
                     YRTKEEIAEWRERDPLAMVEHKLIENDALDEDEIEEIRDEAKERVQEAVKFADESEEP
                     PEEELYTDVYAGEDEYMGEA"
     gene            6970..7959
                     /locus_tag="VN178_00090"
     CDS             6970..7959
                     /locus_tag="VN178_00090"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWS79388.1"
                     /translation="MAETKTYREALREAMVHELDRDENVVLMGEDIGVYGGTHLITDG
                     LYDEYGPKRVMDVPISENGFTGAAVGMAMMGMRPVVEMMTWNFSFLAADQIIQNAAKV
                     RYFSGGQVEVPLVIRGPNGGGVQLSAQHTHSLESFYGHFPGLKVVAPVTPNDVKGMML
                     TAIRDPNPVIFLEAGALYGTKGEVEDGDNAVPFGKARIAREGSDVTLIAYGRQVNLCL
                     RAADTLAEDGFEAEVIDLRSLRPFDEETIVESVEKTHRAVAVQEQWRWFGVASEVAAI
                     IQDRAFDSLDAPIERVSGAEVPGPYARNLELMAFPSEKQVTNAARRVLYEEEK"
     gene            7961..>8477
                     /locus_tag="VN178_00095"
     CDS             7961..>8477
                     /locus_tag="VN178_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWS79389.1"
                     /translation="MPEVIMPKMGDAMEEGTLLKWLKSEGEEVSEGDPIAEIETDKVT
                     MELEAEDAGTLAQLIANEGQDVPVGEAIAFIQGEGEEVPERSEGGGEEEAEEGGEEDG
                     GGAQATATETEAPEEEEAEEEADGRADGRADGHFRASPIVRRLAEENDLDLSKIDGSG
                     PAGRIVERDVRA"
BASE COUNT         1650 a         2613 c         2826 g         1388 t
ORIGIN      
        1 caccgggact ccatggacga ctccatcatc taccgcgcct cccgctacgg caagggagag
       61 gctgcgtacc tgaactgccc gatgacagat gagcagtact acgccttcgt agaggacctg
      121 gctgtcgccg aactaagccc cataaaggac ttcgaggagg acctgtactt cgagggttgt
      181 cttcctgtgg agaccatcgc ccgccgcgga aaggagaccc tccgcttcgg gcccatgaag
      241 cccgtcggcc tccccgaccc gcgaactggc gagatcccct acgccgtcgt gcaactcagg
      301 caggacgacg ccgagggccg cctctacaac atcgtcggct tccagacccg cctgaagtgg
      361 ggcgagcaga ggcgcgtttt ccggacgatt ccaggcctcc agaacgccga tttcgcccgc
      421 atgggcgtca tgcaccgcaa cacctacttg ccctccaatc acatgctaga agccaccatg
      481 cggataagag aagcgctcgg cgaagccccc ctcttcttcg ccggccagct caccggcgtg
      541 gaggggtatg tggagagcac ggcgatgggc cacatagccg gcacgaatgc cgcccggatc
      601 gcccgtggtg aggagccact aaccctgccg cgcggtacca tgatgggggc gctcgcccac
      661 tacatcacca ccaaagaagg caccctccag cccataaact ccaactgggg tctcgtcccc
      721 accgttccga agcgagagaa cggcagacgc ctcagcaaac ccgaacgcag gcaacgccag
      781 gccagaatgg ccctttccac gctcgatgag tttatgcgag agggcgttaa gacagccgtt
      841 tgaacagctg atctccgcac gagctaaccg caggtagaga gggttcggaa tcgctcactg
      901 cggatcgctc tagcgagtgc gtctcgtccg aaaacgttgg gctcccctcc gggctccccc
      961 ggttagcatc acagccgaga ccagcgccac ccacgccaga cccaccaaag ctgccacaag
     1021 agggaagaga aaatgcaaga acgtcagtat tgcgaccagg aacccggcca gagcaagcca
     1081 actcggtaaa acacgtgttc cgaccgccac catcgaggtg gagagcacca tggctgacgc
     1141 ccctatctgg cagtagtggt agagccatga ggagagcgtc agagatacga gcacgaaagc
     1201 ggtgtcagat gagaggtctc cgaagcgttg cagaaccgct ggatagaaga tctctactgt
     1261 gaaccctgcc gccgagagcg tcgcgaatac gatccccccg gcgagcgcca ccgccggtag
     1321 tccctctgct actgccctgg tctcgccccc gggactcctt agcatcccgt acaacacccc
     1381 aaggaacagg aggaagaaga agacgtggaa gagaggtaga accccattgg agaaaacgaa
     1441 catatccgac tcggcatcaa agtaggtctg ggcttcgagc agggagccgg gggcgtccgg
     1501 atacccgacc aggtaggccg gtaccatcga gaccaccgcc agcaacccgg agaggccgcc
     1561 caaacgtagc gggttccctt cagccatgta ctagtcgcct cccggtgtcg ccgttcttgt
     1621 ggggtgaagt ataagcgtaa ccatgagtgt ggcgcttgtc ctgtttccac tctcgaactc
     1681 tcgtgcgtgc tgcggggcaa acgcttcgct atgcttaatc atagctggcg gccacgcagg
     1741 gaggcggcga tgtcggagac gaggtcggag aaggagatac ccgaagcctg ggttggacag
     1801 cgcgtcaggg tgggccacgc ccacggcgcg tccacccaca cggacggggt gttgcttggg
     1861 gttggcgacc ggggcatcgt tgtcgagggc ggcgagctgg tccgcttcta cccctggacg
     1921 tcagttggga gtatcgagaa ggcccacgaa gggggtaccg aggatcccgg cgtacaggcg
     1981 agacgggcct ggtagagact gcctccaagc aatagacggg ctggcctgcg tgaggaagct
     2041 gcaggcagcc gttctacccg gcgggcggta agggtttgtg gcccacgaag acctcgacgc
     2101 gctcgccgcc ggtcgacggg tcttcgacga cttctggctc gaacccgacg tcttcgagca
     2161 atcgtagcca ggtcgcgcgt gggaagaccc ccacgacctg acggtccacg acggcacgta
     2221 gttcgtcgcc atctttcaga gccagcatga actcgtagtt gactttggtg tcgttcggat
     2281 ctgcgtccca ggtccactcc aggtacctga cgccccgatc tcctctgtct accccttcgt
     2341 gggtggtgcc gggctggaag gtctcggcca cccagtcggg tacgaacacg gcgaccccac
     2401 ccggcctgca gtgttcgtgg gcggtgagca gggccgcgcg cagatcctcc tcgctgctca
     2461 tgtacatgat ggcgtcgtga ataaggatgc cgtcgaacgt cctgtccagg cgcaacgtgc
     2521 gcatgtcgcc ctcgatgtgt tcgagctcag ggttttgctt cttgctctgg gcgagcatcg
     2581 cgccagaaat gtccgtgagc gtgagatcga agtccttctt tagaaaccaa gcgacgttgc
     2641 cgccgccgca gcccagctcg agcacggtcc cggcgccctc ggcccggagg agcttcgcga
     2701 agaaagcagc ttcgtccgcg tagtcttcgg tcggcgagat caactgccac cagcctgcaa
     2761 gttcggcgta caatttcggg tgttcggagc ccataatgca acgttaccac gtccccgccg
     2821 taacccaggg acgaaaggct tatagcttcg ccgcggcctc cgtacgtaac gccgcttcga
     2881 ggatggtctc gacgagcgtc tcgaaggtgg tgcccgtggt ttcggccgcg agggggaagg
     2941 tcgatgtctc ggtgaagcca gggatggtct ttacgtcgag gatccaggga gtgcctcccg
     3001 cgaagatggt gtccaccctg gcgaatccgg agcagccgag ggtcttgtag acggtgagcg
     3061 ccgtctcttc gacgcgggtg gcggcctcgt gcggtatctc ggcggggatg gcgaagtccg
     3121 tcgccccggg agtgtacttc gcctcgaagt tgtaggcgcc ttcgcgaggg cggatctcga
     3181 ccaggcctag cgcccttggc tcttcgccag ccggttcgat gatggggatg gagatctccg
     3241 tcccctccac ccaatgttcg agcaggatct tggcgtcgta gcccagcgct tcgtagacgg
     3301 cgtcgaacag tccttcggcc ccctcgaccc gggagagccc gaagctcgac ccctccctgg
     3361 caggcttgac gacgagcggg taaccgagct cctcggcggc gacatccagc gccgcagaag
     3421 cccccatctc gttcattgcc ctcctaagga aggtgcggaa aggcggagtc tgtatgcctg
     3481 cggcgcggag cgcgcgtttg gcgtagtcct tgtccatgca gcggatggaa gagagcggcc
     3541 cgctccccgt gtaggggatg cccagggtct ccagcagggc ctgcaccgtg ccgtcctcgc
     3601 caccggggcc gtgaaggcag atgaaggcgg cgtcgggtga gatctccatc aacgcgcccg
     3661 tagtggagtc ctctatgtcg agcgggtgaa cgtcgtggcc gaggcgttcg agggcgtgct
     3721 ggacgcgtcg gcccgtaatg aaggagacat cacgctccat cgaatgccca ccgcgcaaaa
     3781 cggctacgcg cgccactgta ccctaaccat ccgtgcccga accgaaggcc gccgcgcggc
     3841 cgccgtgtat cgcgctctct tttcggtgcg agccacgttc cagatcgctc ctgagctcca
     3901 tgcgctcccg gatcacctcg gagagcagct ctatgccgcg ccggatcctc tctggttcga
     3961 cgaacgagaa gttgagccgc atgttgttct tgcccgtccc tggagcgccg ccgtagaatc
     4021 cctcgccggg aacgtaggcg acgttcctgg ctatggcgcg ggggagcatc gcggtcgcgt
     4081 ccaggtacga ggggagcgtg gcccacacga agagcccgcc ctcggggtgg gtccagtaga
     4141 cctctttggg catgaactcg gccagagagt cgagcatcgc gtcgcggcgc tctttgtaca
     4201 tggacgtaag gcgcctgacg taggccctcc agtccgcatt ctggaagtag gaggagatca
     4261 tcatctgaga caggttcgag gagcagaggt cggcgccctg cttgccgacg ttgatcttgt
     4321 gcagtatccc cggctgggca tgcacccacc ccagcctgac gccgggggcg aagatcttgg
     4381 agaacgtacc cagataaacg accctgtcga gatctccctc ttcctgctcc aaggccgcga
     4441 gggaagggag cggctcgccc tcgaagcgga gcatcccgta ggggttgtcc tccacgatca
     4501 ccaggtcgaa ctcgcgcgcc atctccaaaa gctccctgcg ccgctcggcg accatcgtta
     4561 ccccagcagg gttctggaag ttggggacgg tgtagaggaa cttgacgtgg agcccacctt
     4621 tgcgggccct cttgagggtc tcgcgggcgg cgacggggat gatgccggcc ctgtccatcg
     4681 ggacgtgggc tatgcgcggc ctgtaggagg cgaaagcgtt gagcgccccg gcgtaggtcg
     4741 gaccctcgca caaaacggcg tcgccctcgt cgaggaagac cttggcgatc aggtcgagtc
     4801 cctgctgggc ccctgtcgtg acgaagacgt cctcctgcct cgccggagtc ccctccgcgc
     4861 ccatgacctc gactatgacg tcctttatgg cttcgagacc cgccgtcggc ccgtactgca
     4921 gggcctgggc cgcgtccgaa gcgatgttgc gggagatctc acggaacgcc tcctccccaa
     4981 aagccctcgt gtccggaaag ccgccggcca gcgagatgat gccaggccgc gagagcgtgg
     5041 ccatcaggtc gcgggtcgcc gccgtcttca tgcccttgac gcggttggcg tagagaaaat
     5101 cgaaccggtc gaggtctacg agcggcaagg attatctccc tcctcggtga cccagcgtcg
     5161 catccgggcc ccccgcgact cgagcttctc caccctcacc tcatgcatgg acgcgactgt
     5221 atcataaaga atcgctcttg tagactccca ggctatgaag gccatcttcg tgactaccaa
     5281 cgaacacaag cgtcgcgagg tacaggagat cctgggcttc gagctggagc gggcggatct
     5341 ggacctgccc gagatacagg ccatcgaacc cgccgaggtc gccgcacaga aggcccgcgc
     5401 cgcccgcgaa gctctgggta gaccggggct accggtcctc gtcgaggatt cgggtctcat
     5461 ggtcgacgcg tgggacggct ttcccggggc gctcacgaaa tggctcatgc agagcgtcgg
     5521 caacgagggc ctgctcagga tgttggcgcc gggcgaggac cgttccgcca gggccgtttg
     5581 cgtcgtcgcg ctcgcggagg ctggcgggac cgtgcgcacg ttcaggggcg aggtgccggg
     5641 aactctggcg cagaggccgc ggggtaccgg gggattcggc tatgatcccg tgttcgtccc
     5701 cggttggtct tccatgacct acgccgagat gggcgaaggc aagaacaggg attcccacag
     5761 gacgcgggcg ttcagggccg tgcgggagtg gttgggaggg gcgtagaaaa gggtataggt
     5821 acgtatggag agtacctaca gaacccgtgg ttttggtatt ctgtcattag gtttgtacct
     5881 ctgagaggga aggggccgtt gatgtcggca gataacgagc actcggaaga cgggggcata
     5941 gagacccgcg aagacggtgg tgccatagcg accagctcgg agcgtctcgt ggacttgtac
     6001 cgccagatgg ccctgatccg ggccttcgag gaggcgtgcc agaggggttt caggcagggt
     6061 aagatcgggg gctacctaca cgtctacatc ggtcaagaag cggtggcaac gggctttctg
     6121 gacgccttca aggagggcga caggatcatc acggcctacc gcgaccacgc gcacgcgctg
     6181 ctcctgggca gcgaccccaa cgaggtcatg gccgagctct acggtaggga gacgggcctc
     6241 gtcaagggca agggcggctc catgcacctc ttcgacgtcg agcggggcct gatgggcggt
     6301 tacggcatcg tcggcgggca catacccctg ggcgtaggcg tcgcctacgc catgcgctac
     6361 caggagactg accacatctg ccagctctac ctgggtgacg gtgcgatgag ccagggtgcc
     6421 ttccacgaag ccgccaacct cgcggggctg tggggcaaag agggcctgtg cccctgcctc
     6481 ttcatcatcg agaacaacaa gtacggtatg ggcacctcgg tagagcgcac gacggccatg
     6541 actgacctcg ccgccaagtt cgacgcctac ggcatcgagc acgagaaggt cgacggtatg
     6601 gacgtcgagg ccgtggtcga ggtcgccgaa agggtggccg ggcaggtgag ggagaccggt
     6661 aagccctacg cggtcgaggc gataacctac aggacggccc cgcacggcgc cgccgacttc
     6721 ttcgagaagt accgcaccaa agaagagatc gcagagtggc gcgagcgaga tcctctcgcg
     6781 atggtcgaac acaagctgat cgagaacgac gcgctagacg aggacgagat cgaggagata
     6841 agggacgagg ccaaagagcg ggtacaggag gccgtgaagt tcgccgacga gagcgaggag
     6901 ccacctgagg aggagctcta caccgacgtc tacgccggtg aggacgagta catgggggag
     6961 gcgtaggcca tggcggagac gaagacctac cgcgaggccc tgagagaggc gatggtccac
     7021 gagctcgaca gggacgagaa cgtcgtcctg atgggcgagg acatcggcgt ctacggcggc
     7081 acccacctaa taacggacgg cctctacgac gaatacgggc ccaaacgggt aatggacgtg
     7141 ccgatctccg agaatgggtt tacgggcgcg gcggtcggca tggcgatgat gggtatgcgc
     7201 cccgtggtcg agatgatgac ctggaacttc tcgtttctgg cggcggacca gatcatccag
     7261 aacgccgcca aggtgcgcta tttctcgggc ggacaggtgg aggtgccgct cgtgatccga
     7321 ggccccaacg gcggcggtgt ccagctctcg gcccaacaca cgcactccct ggagagcttc
     7381 tacggccact tccccggtct caaggtagtc gcgccggtga ccccgaacga cgtcaagggc
     7441 atgatgctga cggcgatcag ggaccccaac cccgtgatct tcctggaggc tggcgccctg
     7501 tacgggacga agggcgaggt cgaagacggg gataacgcag tccccttcgg caaggcccgt
     7561 atcgcccgcg agggctccga cgtcaccctg atagcctacg gccggcaggt caacctatgc
     7621 ctgagggccg cggatacgct cgccgaggac ggcttcgagg ccgaggtcat agatctgaga
     7681 tctttgagac ccttcgacga ggagaccatc gtcgagtccg tagagaagac acacagggcc
     7741 gtggctgtcc aggagcagtg gcggtggttc ggggtggcga gcgaggtggc ggcgatcatc
     7801 caggatcggg ccttcgactc gctcgacgcc cccatagagc gggtgagcgg cgccgaggtc
     7861 cccggaccgt acgctcgcaa cctcgagttg atggcattcc ccagcgagaa gcaagttacc
     7921 aatgcggccc gcagggttct gtacgaggag gagaagtaag gtgcctgaag tcattatgcc
     7981 caagatgggc gacgcgatgg aggagggcac cctcctcaag tggctcaaga gcgagggcga
     8041 ggaggtctcc gagggggacc cgatcgcaga gatcgagacc gacaaggtca cgatggagct
     8101 cgaggccgaa gatgcaggaa ctctcgccca gctgatcgcc aacgaaggcc aggacgttcc
     8161 cgtcggcgaa gcgatagcgt tcatccaggg cgagggcgaa gaggtccccg agcgtagcga
     8221 gggcggcggg gaagaagagg ccgaagaggg cggcgaggaa gacggcggcg gggcccaggc
     8281 cacggccacc gagaccgaag cgcccgagga agaggaggcc gaggaggaag ctgacgggcg
     8341 ggccgatggc cgggccgacg ggcacttcag ggcctctccc atagtccgca ggctggccga
     8401 ggagaacgac ctcgacctct cgaagataga cggctccggc cctgcgggcc gcatcgtgga
     8461 gcgcgacgtg cgggccg
//
LOCUS       DATMVF010000003         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_597791, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000003 DATMVF010000000
VERSION     DATMVF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597791"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VN178_00100"
     CDS             <1..713
                     /locus_tag="VN178_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79390.1"
                     /translation="AVLITLVAMALVGFAFGRKGEEVGPVLYDRNLILIYISAIAILW
                     NLWFFGFWSVSIVSQAAGQGSLLYAGLTAAFNTGAGILGFPAGGWLADYAKRKGWGRK
                     WMLVAFSLIAGLLTVVFGLYIINGGQSLFVMGILLFFQATFFFALQPISMALTADIVT
                     NPALLGAAFGMWNLVGEIGAVLSPAVSGVLRGATGDWNTAVMLDAGILLASAVILLFV
                     REPRAASAQQPSETEARS"
     gene            747..>2067
                     /locus_tag="VN178_00105"
     CDS             747..>2067
                     /locus_tag="VN178_00105"
                     /inference="COORDINATES: protein motif:HMM:NF039390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79391.1"
                     /translation="MRLRRSTRQVGEDEAIPASLPAFFGNATVGGGPALVRAYIEELL
                     PDVLEGRIEPGRVLARTVGLDGVPDGYRAMDDRESIKVMVSMRDKERLLSPDEPLRFI
                     FSHSALREGWDNPNVFQICTLNEARSEVKKRQEIGRGLRILVDETGERVFDASYNRLT
                     IVANESYAEFARALQTEIRDEIGVDFSGRTKNVRDRRTASLKGGWKLDADFRELWERI
                     KHRTRYRVEYDTDELIRKAAESIKDSMAPIEAPKIVIRKGALSFGDFEEKGLTTSMLS
                     ADEVDVLGESVRIPDILGHIQRETELTRSTIARILVKSGRLPDVTKNPQQFLDRATKE
                     IRKALDGMMIDGIKYERVAGQEYEMRLFADKEITSYVEKMVDTEKSIYDSFPLDSMPE
                     QNFVRDLNTREDIRFFLKLPNWFKIEPPIGTRWRSSCRSGAVGGMSWW"
BASE COUNT          398 a          596 c          683 g          390 t
ORIGIN      
        1 tagcggtgct cataaccctc gtagccatgg cgctggtcgg gttcgccttc ggcaggaagg
       61 gtgaggaggt aggcccggtg ctgtacgaca ggaacctgat cctgatctac atctcggcga
      121 tcgcgatcct ctggaacctc tggtttttcg gcttctggtc ggtgtccatc gtctctcaag
      181 cagcggggca aggatctttg ttgtatgccg ggttgacggc tgcattcaat actggggccg
      241 gcatacttgg gttccctgcc ggcggctggc tggccgatta cgctaagcgc aaaggatggg
      301 gccgcaagtg gatgctcgtg gctttctcgc tgattgcggg actcttgacg gtcgttttcg
      361 gcctctacat catcaacggg gggcaatctc tcttcgtgat gggcatcttg ctgttcttcc
      421 aggccacgtt cttcttcgct ctacagccga tctccatggc gctcaccgcg gacatagtca
      481 ctaaccccgc ccttctcggc gcggcatttg gcatgtggaa cctggtcggc gagatcggtg
      541 ccgttctttc tccggccgtt agtggtgtgt tgcgaggcgc gacgggggac tggaatacag
      601 cggtgatgct ggacgcgggt atcctcttgg cgagtgccgt gatactgctg ttcgtgaggg
      661 agccgagagc ggcctcggct cagcaaccct ccgagaccga ggcgaggagt taagggcttg
      721 ttcgcaaggt ccaaagccgc gtagcaatgc ggctccgaag gtcaacacgc caggtaggcg
      781 aggacgaggc gatccccgcc tcgctgccgg cgttcttcgg caacgccacc gtgggcggcg
      841 ggcccgccct ggtccgggcg tacatcgagg agcttttgcc ggacgtgctg gagggcagga
      901 tcgagcccgg ccgcgtcctc gcccggaccg tgggcctcga cggggtgccc gacggctaca
      961 gggcgatgga cgaccgcgag tcgatcaagg tcatggtgag catgcgcgac aaggagcgcc
     1021 tcctgtcacc cgacgagccg ctgcgcttta tcttctctca ctcggccctg cgtgaggggt
     1081 gggacaaccc gaacgtcttc cagatatgca cgctcaacga ggcccgctcg gaggtgaaga
     1141 aacggcagga gataggtcgc ggcctccgca tactcgtcga cgagaccggt gagcgggtct
     1201 tcgacgccag ctacaaccgg ctgaccatcg tggctaacga gagctacgcg gagttcgcaa
     1261 gggcacttca gaccgagata agggacgaaa ttggcgtcga cttctcggga cgcacgaaga
     1321 atgtccggga ccgccgcact gcctcgctca aggggggttg gaagctggac gcggacttcc
     1381 gggagctttg ggagcgcatc aagcatagga cccgctaccg cgtcgagtac gacaccgacg
     1441 agctgatccg gaaggccgca gaatcgataa aggactccat ggcacccatc gaagccccga
     1501 agatcgtcat tcgcaagggc gccttgagct tcggagattt cgaggagaag ggcctgacca
     1561 cgagcatgtt gtccgccgac gaggtcgacg tgctcgggga gtcagtgcgc atcccggaca
     1621 tcctcggcca tatccagagg gagacagaac tcacgcggag taccatcgcc cggatactcg
     1681 tcaaaagcgg tcggctcccc gacgtgacga agaacccgca gcagttcttg gaccgggcga
     1741 cgaaggagat aaggaaggcg ctcgacggca tgatgataga cggcatcaag tacgagcgcg
     1801 tcgcgggcca agagtacgag atgcggctct tcgcggacaa ggagataacg agctacgtgg
     1861 agaagatggt cgacacggag aagtccatct atgactcctt ccccttggat tccatgcccg
     1921 aacagaactt tgtccgggac ctgaacaccc gcgaggacat caggttcttc ctcaagctgc
     1981 cgaactggtt caagatcgag ccccccatcg gcacgaggtg gcggagttct tgcagatcgg
     2041 gcgcagtcgg gggtatgagt tggtggg
//
LOCUS       DATMVF010000004         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_368487, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000004 DATMVF010000000
VERSION     DATMVF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368487"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..943)
                     /locus_tag="VN178_00110"
     CDS             complement(41..943)
                     /locus_tag="VN178_00110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HWS79392.1"
                     /translation="MIDEIRALLPRHLPGYEVRSIIRLGEGLDNAAYEVNGELIVRAS
                     RETDPALRSQSTQRNANLLAVVAGLSTLPVPEPVFVAAEAGVLAYTRLPGLPLMEHPV
                     AEPARLASTLGGFLSRLHQAPLEKVEKLVEQDTYPLLAWLEDAQRDYQEIVEHLPAAA
                     RPLVEDFLGRTPPAEPLAAPALFCHNDLGAEHVLVDAGASAVTGVIDWSDAAIADPAR
                     DLALIYRDLGPKIFDLMLAQYEGRFDDAERERAVFYARCKVLEDIAYGVRTGARPYAV
                     AGLAHLARTFVGRDGAGARKIRTP"
     gene            complement(1008..1766)
                     /locus_tag="VN178_00115"
     CDS             complement(1008..1766)
                     /locus_tag="VN178_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS79393.1"
                     /translation="MTTYVLVGGAWLGGWCWQRVARRLRDNGHDAYPVTLTSLGERVH
                     LASPQVDLETHITDVVNLVEFEDLHDVVLLGHSYGGLVVTGAADRIPERISQLVYLDT
                     APLPDGAILLEKFPLEARERTEDQVQELGEGWKFPMPPPEELANMASLEGVDEDRLRL
                     LYSRATSQPFGTYTQPLRLKNPAREGLPKLGILCSFSLDQLQEMIASGNPLFRELDGP
                     EWRFVELPTGHWPMFSRPEDLAKLLFDLPPEEPG"
     gene            complement(2061..2213)
                     /locus_tag="VN178_00120"
     CDS             complement(2061..2213)
                     /locus_tag="VN178_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79394.1"
                     /translation="MSGSVEFVAKMGLDEPEHPDCRPLETRLPRSSDRFIVGVTPMVI
                     WGSYRR"
     gene            complement(2237..2656)
                     /locus_tag="VN178_00125"
     CDS             complement(2237..2656)
                     /locus_tag="VN178_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS79395.1"
                     /translation="MGREQIEMTVRTTVEELLSRMAAGDAERIAELFAEEVDFMCAGS
                     EKVPWIRPRRTRRDMADFFSSINENFVPEDRSASVSRCLVDGKDAVVMGHVSQRLKSN
                     GKAFTIPFALHLSVSNGLITRYHVYEDSLTVAKAVTA"
BASE COUNT          536 a          925 c          910 g          528 t
ORIGIN      
        1 tcggtagtcc cggcgcaggg tggggcgggt aggtagccgg tcagggtgtt cggatcttcc
       61 gtgcaccggc gccgtcacgc cctacgaagg tccgggctaa gtgtgcaaga ccggctaccg
      121 cgtaaggacg tgcgccagtc cggacgccat acgctatgtc ctcaaggacc ttgcagcggg
      181 cgtagaagac ggcccgctca cgctcggcgt catcaaagcg gccctcgtac tgggccagca
      241 tcaggtcgaa gatcttggga ccgagatctc ggtagatcag cgccaggtcg cgagcgggat
      301 cggcgatggc agcgtctgac cagtcgatga cgcccgtgac ggcgctcgcc ccagcgtcca
      361 ccagcacgtg ctctgcccct aggtcgttgt ggcagaatag agcaggagcg gcaaggggct
      421 ctgccggcgg cgtgcggccg aggaagtcct cgaccagagg gcgagccgcc gcgggtaggt
      481 gctccacgat ctcctggtag tccctctggg cgtcctccag ccacgccaac agcgggtaag
      541 tatcctgctc cactagcttc tccaccttct ctaagggagc ctggtgcagg cggctcaaga
      601 acccgcccag ggtggacgcc aggcgcgcgg gttcggctac aggatgttcc attaggggaa
      661 ggccgggtag cctggtatac gcgagtacgc ccgcctcggc agctacgaag actggctcgg
      721 gaaccggcag cgtggagagt ccggccacca cggccagcag gttcgcattg cgctgagtcg
      781 attggctgcg gagggcggga tcggtctctc tgctcgcccg gactatgagc tcgccgttta
      841 cctcgtaggc ggcgttgtcg aggccctctc ccagcctgat gatagatcta acctcgtagc
      901 caggcaggtg gcggggcagc aacgcgcgga tctcgtcgat cacggttgca cctcagagcc
      961 cgtttcgcaa gcctcaggta ggccgccgaa gacccacggc catcgtctca gccaggctcc
     1021 tccggcggga ggtcgaagag cagcttggcg aggtcctcgg gcctggagaa catgggccag
     1081 tgccccgtgg gcaactcgac gaaccgccac tcgggaccgt cgagttcgcg gaacaagggg
     1141 ttgccgctgg cgatcatctc ctgcagctga tccagcgaga agctgcacaa gatccccagc
     1201 ttcggtagcc cttctcgggc cgggttctta agccgcaagg gctgggtgta ggtgccaaac
     1261 ggctgcgagg tggcccgcga gtacagcagc ctaagacgat cttcgtccac cccttccagg
     1321 ctggccatgt tggccaactc ttccggcggt ggcatcggga acttccagcc ctcgcccaac
     1381 tcctgcacct gatcctcggt tcgctctctg gcttcgagcg ggaacttctc aagcaggata
     1441 gcgccgtcgg gaagcggcgc ggtgtccagg tacactagct gggagatgcg ctcggggatc
     1501 ctgtccgccg cgcccgtgac cacgagccca ccgtagctgt gtccgaggag cacgacgtcg
     1561 tggagatcct cgaactctac gaggttcacc acgtcggtga tgtgggtctc caggtcgacc
     1621 tggggagagg cgaggtgcac ccgctcgcca agactggtga gggtaacggg gtaggcgtcg
     1681 tggccgttgt ctcgcagccg acgggcgact ctctgccaac accagccccc cagccaagcg
     1741 ccaccgacta acacgtaagt tgtcatccgc aagacctcct aatagatcag tgtacaaggt
     1801 catctcgaac ccttacctct aaaaacaagc tcttaggact ggcgacaccg gcagcagaac
     1861 ccggaacggc tctgatagcc tcgcctcgct gcgcaagagc ctacccgtca ggtcctgacc
     1921 cacagaagag caaccggcaa tgccgaaggg gagaagcagt gcggctccgc ctacgagtcc
     1981 caacttcagg aactctccgc gcgagatgtt cattgatgag tcgatccttt cgttgttcgg
     2041 caaccacgcg ccacagtcgt tcagcggcga taactacccc agatgaccat cggcgtgacg
     2101 cctacgatga acctgtccga cgatcgtggc aggcgtgtct caagtggacg acaatcggga
     2161 tgttccggct cgtcgagtcc catcttcgcc acgaactcga cagagccact catcagcgtg
     2221 gctctctgtc gcctcattag gccgtgacag ccttggcgac cgtcaggctg tcctcgtaga
     2281 cgtggtagcg ggtgattagg ccattggaga cgctcaggtg cagcgcgaac gggatcgtaa
     2341 aagccttgcc attggacttc agccgttgcg aaacgtgccc cattaccacg gcatccttac
     2401 catccacgag gcacctggat accgacgccg acctgtcctc cgggacgaag ttctcgttta
     2461 tcgaggagaa aaagtctgcc atgtcccggc gggtccgtcg gggacgaatc catggcacct
     2521 tctcggatcc ggcgcacatg aagtcgacct cctctgcgaa cagctcggcg atcctctcag
     2581 cgtcgccggc tgccatcctg ctcaacagct cctcgacagt ggtacggacg gtcatttcga
     2641 tttgctctct tcccaagacg gatgctcttt gcgcggtcat tatcttcgct gccaggccgc
     2701 ctttccatta cctcgcaggt aatgtcgttg ccgcggccga cgatgtacca ttgctggcat
     2761 ggaaggccgg gtgaccacga tggagcaaca ccggcggccg gtcggcgagc tactgcgccg
     2821 gtggcgagga agacggcgcc tgagccagct cgagctcgcc cttgagtcgg ggatctccgc
     2881 gaggcatctg agcttcgtg
//
LOCUS       DATMVF010000005         7496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_460894, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000005 DATMVF010000000
VERSION     DATMVF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460894"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="VN178_00130"
     CDS             complement(<1..1859)
                     /locus_tag="VN178_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWS79396.1"
                     /translation="MQNGNLLRIGRRRKRVRKKSKLLQRLRTALLLLCFASILGVVGM
                     VASAVSTYQALAANMPDLDDYHSTELAQTSLIYDTEGNIVDQLYGVQNRFVVPLEEVD
                     PTLRQAIISIEDHRYYEHRGLDFEAIGRAAVENVKSLSIQEGGSTLTQQLIKNTYIAQ
                     QERQVASFERKFVEGSLAWQYEKEHPKDEILEQYLNTVYFGSNAYGAEAAAKTYFDKQ
                     ASDLTLPESALLAGIINLPGVYDPFVDPESAKARRNVVLDRMLEYGHITEKEHDRAVA
                     SKLDVSRGRVQYENDNEYFLNAVRKEIAEEYGDDMVYEGGLKIYTTLDPDLQAMANDA
                     VDSVVNPEAGDPSASLVSIQPGSGAVRAMVGGSDFDQVKFNLATQAHRQAGSSFKPFV
                     YSEAVHEGISPETLYVSKHLTIDMGRNAKPYEVDNYDFIHRGPITLEKALADSDNTVF
                     VQLALDLGLQKVVEMANELGIDSEVDAYPSTAIGGLRVGVTPLEMASAYSTFANSGTH
                     MEPYLVEKLTKDDKGKDTLLEEHRPFGEEVLSRDEAAVVTEALRRVTESGTASYYHDL
                     EAEIGRPSAGKTGTSNEFIDAWFIGFIPQLSTSVWVGYPNERRSMTNINGLPEV"
     gene            1938..3050
                     /locus_tag="VN178_00135"
     CDS             1938..3050
                     /locus_tag="VN178_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWS79397.1"
                     /translation="MFPALATNDCAYLNTGSSGPPPDYVLAAMREVDDVCSGRAYLEG
                     FGLFVHQAETNSRARQAAARLVGADDVALTQNTTHGMNLGVGSIDWREGDEVVSVTTE
                     HPGCLVPLHNLKNRFGVVVDLIPPPVTPEKIEASLTPRTRLVALSHVDWTNGRVLPLR
                     EVCAVTRERGVLTLVDGAQSVGNIPVDVPATGADMYAFTGHKWVLGPEGIGAFYVRPG
                     LQVYSPNVGIMSLNDPSDFDNEGGYGLRSDARRFEASTMSPALAGGLAAAADAVYERG
                     GAWFEEIRHRADFLIDLLSELPRVTIRSPRPARSGLVSFEVEGVAAEDAVERLLEQGF
                     ILRYLPAPNSYVRASTHLFNTDEELEALAKAVRALS"
     gene            complement(3072..3719)
                     /locus_tag="VN178_00140"
     CDS             complement(3072..3719)
                     /locus_tag="VN178_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWS79398.1"
                     /translation="MISAVLFDFDGTLVDTTEMIHQSMRHATSSVLGREDIPRETLLA
                     NVGQPLPRQMELIDTENAESLLEAYRSHHERHHDALIREFPGVEESLSRLRSAGIKVA
                     VVTSKRRVSVEMALEIFPGLRNVVDRFVTMEDTTHHKPHPEPLLRALKLLGSIPKERA
                     AYVGDSPFDVEAAKAAELTSVAVSWGAFSEDALRASGPDHLVPDIRSAVDILSTL"
     gene            complement(3723..4541)
                     /gene="surE"
                     /locus_tag="VN178_00145"
     CDS             complement(3723..4541)
                     /gene="surE"
                     /locus_tag="VN178_00145"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWS79399.1"
                     /translation="MRIVLTNDDGIAAPGLLAARRALEKVGDVITVAPDQNRSGVGRS
                     ISFGRSLHVEEREMADGVIGYACTGTPVDCVRLVALGLMDFEPDVVVSGINHGENLGD
                     DITYSGTVAGALEGIVIGVPGIAVSLSINRPWHETVQDTIPLDDRLHFDAAADFTARL
                     VKVAFKGLPSGRILNVNAPNLPRERLRGARVTRLGRRFYKDELVEVRDEHGRVGYDIY
                     NNPPGHHEEEGTDFAAIENDEISITPVHLELTDKAGLEELGAWDVDGLLRNGLD"
     gene            4762..5748
                     /locus_tag="VN178_00150"
     CDS             4762..5748
                     /locus_tag="VN178_00150"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HWS79400.1"
                     /translation="MNAVEWFKHRSYDYKRFSDLEALGSRKRDLGLTVSAVLPGRNVA
                     DTVGGIVDEIHAVNYQAGDHPLVDQILVVDADSPDGTADVAASKGAEVYSENELMSNH
                     GGAHGKGDAMWRALSVARGDLVMYIDADTKDFKPQLVYGILGPILEVPEIRFVKAAYR
                     RPFKSYETVEQDGGGRVTELSAKPLFNLFYPELSGFVQPLAGEFVADRELFSSIPFLT
                     GYAVETGIMIDVLKVVGLEAMAQVDLGTRQNRHQPLRDLSRMAYSVLRAVARRMRQEG
                     RLNQVRDPGMPDSLFQLSDYLHAVATPDGLMLQEYVEELVERPPIKEVLRVG"
     gene            5759..6058
                     /locus_tag="VN178_00155"
     CDS             5759..6058
                     /locus_tag="VN178_00155"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79401.1"
                     /translation="MAPVHDMGGSPGGPIDKDQHEVEDWERLADAINSALGGRGLQTT
                     DELRRSIESLEDYRNLSYYERWAAATEKLLIEKGVLSGDEIDERAAEIRRRWEGP"
     gene            6055..6345
                     /locus_tag="VN178_00160"
     CDS             6055..6345
                     /locus_tag="VN178_00160"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HWS79402.1"
                     /translation="MTFEPGDRVRVLPSEKPGHVRTPAYLKGKTGWVESLIGAFPNPE
                     EIAYGLSGRPERPLYKVCFRQTDLWDDYAGPAEDRLYADIYEHWLEPERGST"
     gene            6342..6947
                     /gene="nthA"
                     /locus_tag="VN178_00165"
     CDS             6342..6947
                     /gene="nthA"
                     /locus_tag="VN178_00165"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HWS79403.1"
                     /translation="MSDHHLHEETGGTTSPYAARIRAIEALLLEKGVLTEPEVQDNIS
                     YMEARSPANGARLVARAWVDPDFKELLLSDGKAAASELGMDPEHPAEFIVVENTPELH
                     NLIVCTLCSCYPRAILGRPPDWYKSFAYRSRAVREPRAVMREFGFEPPEGVEVIVHDS
                     TADARYMVLPMRPPGTENLSEDELAELVTRDSLVGVNVPRL"
     gene            complement(7105..>7496)
                     /gene="fusA"
                     /locus_tag="VN178_00170"
     CDS             complement(7105..>7496)
                     /gene="fusA"
                     /locus_tag="VN178_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWS79404.1"
                     /translation="FHSVDSSENAFKVAGSMAFKNAVEKANPVLLEPYVKVEVLTPSE
                     LVGDIMGDLSSRRGRPMGVEQRGERQIIQAEVPQIEMLTYARDLRSITGGRANFHVEA
                     GHYEEVPPNLVEKVLAANEREEEKAVS"
BASE COUNT         1345 a         2356 c         2381 g         1414 t
ORIGIN      
        1 acctcaggga ggccgtttat gtttgtcatg gagcgccgct cgttcgggta gcctacccag
       61 acgctcgtgg agagttgcgg tatgaagccg ataaaccagg cgtcgatgaa ctcgttggag
      121 gtacccgtct tgcccgcgga ggggcggccg atctcggctt cgagatcgtg gtagtagctg
      181 gccgttccgc tctccgtcac gcggcgcaac gcttcggtga cgacggccgc ctcgtccctg
      241 ctaaggacct cctcgccgaa ggggcggtgt tcctccagga gcgtatcctt ccctttatcg
      301 tcctttgtta gcttctctac caggtaaggc tccatgtgcg tacccgagtt ggcgaaggta
      361 gagtaggccg aggccatctc cagcggcgta acgccgaccc tgaggccccc gatcgcggtc
      421 gaagggtagg cgtcgacctc gctgtctatg cccagctcgt tggccatctc cacgaccttc
      481 tggaggccga gatccagggc cagctggacg aagaccgtgt tgtccgagtc ggcgagagcc
      541 ttctctagcg tgatcggacc gcggtggatg aagtcgtagt tgtccacctc gtaaggcttt
      601 gcgttacggc ccatgtctat ggtgaggtgc ttggagacgt agagggtctc gggcgagatc
      661 ccctcgtgta cggcctccga gtagacgaag ggcttgaacg agcttcccgc ctgccggtgc
      721 gcctgggtcg cgaggttgaa cttcacctgg tcgaagtccg agccgcctac catggccctt
      781 acagcgccgg atccgggttg gatggagacc agggaggccg aaggatctcc ggcctccggg
      841 ttgacgacgg agtcgaccgc atcgttcgcc atcgcctgga gatccgggtc cagggtggtg
      901 tagatcttga gcccaccctc gtagaccata tcgtcgccgt actcctcggc tatctccttt
      961 cggacggcgt tgaggaagta ctcgttgtcg ttctcgtact ggaccctgcc gcggctcaca
     1021 tcgagcttcg atgccacggc cctgtcgtgc tccttttcag tgatgtggcc gtactcgagc
     1081 atcctatcga gcacgacgtt gcgcctcgct ttggcgctct cgggatctac gaaaggatcg
     1141 tagacgccgg gcagattgat tatccctgcc agaagggcgg actctgggag cgtgaggtct
     1201 gaggcttgtt tgtcgaagta ggttttggca gcggcctcgg cgccgtaggc gttggacccg
     1261 aagtacacgg tgttgaggta ctgttcgagg atctcatctt tagggtgctc tttctcgtac
     1321 tgccaggcga gcgagccctc gacgaacttc cgctcgaagc tggcgacctg gcgctcctgc
     1381 tgggctatgt aggtgttctt tatgagctgc tgggtcaggg tggagccccc ctcctggatg
     1441 gagaggctct tgacgttctc caccgccgcc cgtcctatgg cctcgaaatc caaccccctg
     1501 tgctcgtagt aacgatggtc ttcgatcgag attatggcct gcctgagggt cgggtcgacc
     1561 tcttccaggg ggactacaaa gcggttctgg acgccgtaga gctggtcgac gatattgcct
     1621 tcggtgtcgt agataaggga ggtctgggcg agctcggtgg agtggtagtc gtcgaggtcg
     1681 ggcatgttgg cggcgagggc ctggtaggta ctcacggcgc ttgccaccat gccgaccacc
     1741 ccgagtatgc tcgcaaagca gagcagcaga agggcggtcc ttagcctctg caagagcttg
     1801 ctcttcttgc gcacgcgttt gcgcctgcgg ccgattctga ggaggttacc gttctgcatg
     1861 aagaggctat tatacagaac ggcgattccg atcccggaag gagaaacacc atcacagaca
     1921 cgagcctccc ccgcgagatg tttccggccc tcgccacgaa cgactgcgcc tacctgaaca
     1981 ccggcagcag cggacctccc cccgactacg tccttgcggc catgagggaa gtggacgacg
     2041 tctgctcggg ccgcgcctac ctcgaaggct tcggcctctt cgtccaccag gccgaaacga
     2101 actcacgcgc ccgacaggcc gcggcccgtc ttgtaggcgc tgatgacgtg gctctcaccc
     2161 agaacaccac ccacggcatg aacctcggcg tcggctcgat agactggcgc gaaggcgacg
     2221 aggtcgtctc ggtcacgacg gagcacccgg gctgcctcgt gccgctccac aacctgaaga
     2281 accgcttcgg ggtagtggtc gacctgattc cgccccccgt gacgccggag aagatcgaag
     2341 cctccctgac gccccggaca cgcctcgtcg ccttatcgca cgtggactgg acgaacggaa
     2401 gggtactgcc gctgcgggag gtctgcgccg tgaccaggga gcggggagtc ctcaccctgg
     2461 tggacggcgc ccaatcggtc gggaacatac cggtcgacgt gccggccacc ggagcggaca
     2521 tgtacgcttt caccggccac aagtgggtgc tcggccccga aggcatcggc gccttctacg
     2581 tcaggccagg cctgcaggtc tacagcccga acgtaggcat catgtcgctg aacgacccca
     2641 gcgacttcga taacgagggt ggctacgggc tgagatccga cgcccgccgc ttcgaagcct
     2701 cgaccatgag tcccgccctc gcaggcggtc tcgccgcagc agcagatgcc gtctacgaac
     2761 gcggcggtgc gtggttcgag gagatccggc accgcgccga cttcctgata gacctcctct
     2821 cggagctacc gcgcgtcacc atacgttcac cgagaccggc ccgatccggg ctcgtcagct
     2881 tcgaggtcga aggtgttgcg gccgaagacg ccgtcgaacg cctgctggag cagggtttca
     2941 tccttcgcta cctgcccgcc ccgaactcct acgtcagggc gagcacgcac ctgttcaata
     3001 ccgacgagga gttggaggcg ttggcgaaag cggtgcgcgc actgtcgtag ggacgggttt
     3061 gaaacccgcc cctacagggt cgataggatg tcgacggccg atcggatgtc ggggacgagg
     3121 tggtcgggtc ctgaggcgcg taaggcgtcc tcggagaagg cgccccagct tacggcgacg
     3181 ctcgtgagct ctgcggcctt cgcggcttcc acatcgaagg gggagtcgcc tacgtaggcg
     3241 gctcgctcct ttgggatgct gccgagcaat tttagggctc tcaacaacgg ttcggggtgg
     3301 ggcttgtgat gcgtggtgtc ctccatggtg acgaagcggt ccacgacgtt ccgaagaccg
     3361 gggaagatct cgagggccat ctccaccgag acgcggcgtt tggaggtgac cacggctacc
     3421 tttatcccgg ctgagcggag gcgtgagagc gactcctcga caccggggaa ctcccggatc
     3481 agggcgtcgt ggtgtcgttc gtggtggctg cggtaggctt cgagcagcga ctcggcgttc
     3541 tctgtgtcta tcagctccat ctggcgcggg aggggctggc ctacgttggc cagcagcgtt
     3601 tcacggggga tatcttcgcg gccgaggacc gagcttgtgg cgtggcgcat ggactggtgg
     3661 atcatctccg tcgtatcgac gagcgtcccg tcgaagtcga agaggacggc agagatcaac
     3721 gcctaatcga gcccgttcct gagcaagccg tctacgtccc aggcgccgag ttcttcgaga
     3781 cccgctttat ccgttagctc gaggtggacg ggggtgatgc tgatctcatc gttctcgatg
     3841 gcggcgaagt ccgtgccctc ctcctcgtgg tggcccgggg ggttgttgta gatgtcgtag
     3901 cccaccctcc catgctcatc ccttacctcg acgagctcat ccttatagaa ccggcgcccg
     3961 agcctcgtga cccgcgcgcc cctgagcctc tcgcggggca gattaggggc gttgacgttg
     4021 aggatccggc ccgacggaag gcccttgaag gctactttca ccaggcgtgc cgtgaaatct
     4081 gcagcggcat cgaagtggag cctgtcgtcg aggggaatgg tatcctgcac cgtctcgtgc
     4141 caggggcggt ttatgctgag agagacggcg atgccaggga ccccgatcac gatcccctcc
     4201 agcgcaccgg cgaccgtccc cgaataggtg atgtcgtcgc cgaggttctc cccgtggttt
     4261 atccccgaga cgacgacgtc cggctcgaag tccatgagcc cgagggcgac gagccgcacg
     4321 caatcgaccg gcgtccccgt gcaggcgtac ccgatcaccc cgtcagccat ctcgcgctcc
     4381 tcgacgtgga gcgagcgccc gaaggagatg ctcctgccca ctcccgaacg gttctggtcc
     4441 ggggcaacgg tgatgacgtc ccccaccttc tccagcgccc gcctggccgc cagaagtccc
     4501 ggagccgcga tcccgtcgtc gttggtcaat actatgcgca tagttccaaa ccctccgtaa
     4561 gtccggaggt tgttgtcgtc caaaaggctc ctgattctat catcctggcc ctgatgtccc
     4621 cgcggccggg caatggaatt ggtggtatcg gtataggaat gggctattcc caacattaag
     4681 aaaaaagaat ggacaaagcc gcacatatac ggtagcttac cgaggctgtg atggagagac
     4741 ctacgtaagg agtgtgatgt tgtgaacgcc gtagagtggt ttaagcatcg ctcctatgac
     4801 tacaagcggt tctcggacct ggaggctctt ggctcccgca agcgcgacct cggcctgact
     4861 gtcagcgcgg tcctgcccgg ccgcaacgtc gccgacaccg taggtggtat cgtcgacgag
     4921 atccacgccg tcaattatca ggcgggggat catccgctgg tcgaccagat cctcgtggtc
     4981 gacgctgact cacctgacgg caccgcagat gtggccgcat cgaagggcgc cgaggtctac
     5041 tcggagaacg agctcatgag caaccacggt ggcgcgcacg gcaagggcga cgccatgtgg
     5101 cgcgcactct ccgtcgcgcg gggtgacctc gtcatgtaca tcgacgccga caccaaagac
     5161 ttcaagcccc agctcgtcta cggcatactc ggcccgatcc tagaagtgcc cgagatccgc
     5221 ttcgtcaagg ccgcctacag gcgtcctttc aagagctacg agaccgtgga gcaggacgga
     5281 ggagggaggg ttacggagct ttcggccaag cccctcttca acctcttcta ccccgagctt
     5341 tcaggcttcg tgcaacccct cgcgggggag ttcgtcgccg acagggagct gttctcctcc
     5401 atcccgttcc tcaccggcta cgcggtggag accgggatca tgatagacgt cctcaaggtg
     5461 gtcgggctgg aggcgatggc gcaggtcgac cttggtacac gccagaacag gcaccagcct
     5521 ttgcgggatc tctcccgcat ggcttactcc gtccttcgtg cggtcgcccg gcgcatgcgc
     5581 caggagggac gcctgaacca ggtgcgggac cctggcatgc ccgactcact tttccagctc
     5641 tcagactacc tgcacgcggt cgccacccct gacggcctca tgctccagga gtacgtcgag
     5701 gaactcgtcg agcgaccccc gatcaaggaa gtcctgaggg tcggatagga gcagaacctt
     5761 ggccccagtc cacgatatgg gcgggtcgcc cggaggaccc attgacaaag accagcacga
     5821 ggtcgaagac tgggagcgtc tggccgacgc tataaactct gctctcggcg gcagggggct
     5881 ccagaccacg gacgagctcc gcagatccat tgaaagcctc gaagattacc ggaacctctc
     5941 atactacgaa cgctgggcag cggcgaccga gaagctactc atcgagaagg gcgtcctctc
     6001 cggcgacgag atagacgaga gggcggcaga gatcagacgc cgttgggaag ggccgtgacg
     6061 ttcgagcccg gcgaccgggt cagggtgctc ccctcggaga agcccggtca cgtacgtacc
     6121 ccggcatacc tcaaggggaa gaccgggtgg gtcgagtctt tgatcggcgc gttccccaac
     6181 ccggaagaga tcgcctacgg tctctcaggc cggcccgaac ggcccctgta caaagtctgt
     6241 ttcaggcaga ccgacctctg ggacgactac gcaggccccg ccgaagaccg cctgtacgcc
     6301 gacatctacg agcactggct ggagcccgaa aggggaagta cgtgagcgac caccacttgc
     6361 atgaggagac gggagggacc acgagccctt acgccgcgcg catcagggcc atagaggccc
     6421 tactactcga gaaaggcgtc ctcacggagc ccgaggtaca ggacaacatc tcctacatgg
     6481 aggcccgctc tccggccaat ggcgccaggc tcgtggcccg cgcctgggtg gacccggact
     6541 tcaaggagtt gctgctctcc gatggtaagg ccgcagcttc ggagttaggc atggaccccg
     6601 agcatcccgc agagttcatc gtcgtcgaga acacgccgga actccacaac ctgatcgtct
     6661 gcaccctgtg ctcctgctac ccgcgggcca tcctcggacg tccgccagac tggtacaaga
     6721 gctttgcgta ccgctcgcgc gccgtcagag aaccccgcgc cgtgatgcgc gagttcggct
     6781 tcgaaccacc cgagggcgtc gaggtcatcg tccacgacag caccgcagac gcccgctaca
     6841 tggtcctccc catgcgtcct ccgggcacag agaacctgag tgaggatgag cttgcggaac
     6901 tcgtcacgcg cgactccctg gtcggggtca acgtgcctcg tttatagcat ttcagcctgt
     6961 cagcacggcg gctgcgccgc cttgtcagcc agccacttag ggcttgcgca acctgacaag
     7021 ctgaaatgct gacaagccgc cgaccgaagg gaggcggcgt gctgaaatgc tgacaagcga
     7081 agcccgaagg gctgagcgtg ctgactagct tacagctttc tcctcctccc tctcgttggc
     7141 ggcgaggact ttttctacga ggttcggagg gacttcttcg tagtggccgg cctcgacgtg
     7201 gaagttggcg cgcccgccgg ttatggagcg gaggtcgcgg gcgtaggtga gcatctcgat
     7261 ctggggcact tcggcctgta tgatctggcg ctcgccgcgc tgctcgacgc ccatcggcct
     7321 cccgcggcgg ctggagaggt cgcccatgat gtcgccgacc agctcggaag gcgtcagcac
     7381 ctcgaccttc acgtacggct ccagcagcac agggttggcc ttttccacgg cgttcttgaa
     7441 ggccatagat cctgcgacct tgaacgcgtt ctcggaggag tcgacggaat gaaacg
//
LOCUS       DATMVF010000006         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_230567, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000006 DATMVF010000000
VERSION     DATMVF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230567"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VN178_00175"
     CDS             complement(<1..730)
                     /locus_tag="VN178_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HWS79405.1"
                     /translation="MTITIPSGGVQQTEKLMTGTQAVAQAVRLADVDVMAAYPIRPYD
                     GVMQAVAKLIANGELDAEYIVAEGEHSQFEICKHASAVGSRVFVGSSGVGWMYAMESL
                     AVTPPLRLPVVAMIGNRALDDPGAFGVEHNDAMCVRDLGWQLFWVEDAQECFDATLMA
                     YRVGEDKRVSFPVGLGVDGAFITHSQALVKIPDQKSVDAYLPPYDLGDRLLHPDNPIS
                     IAPQANEDWVMEIRKQTDEAARRVK"
     gene            complement(741..1916)
                     /locus_tag="VN178_00180"
     CDS             complement(741..1916)
                     /locus_tag="VN178_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HWS79406.1"
                     /translation="MPTGREAGDSMPGDQDTIEGRTSTEVRDEAKSVEFTYRGIFQKT
                     LAANLCKHVVMAARKRDWLGSTIQRYSDSPERNGIPAKQFAVIAPDTETLEGHMAKYE
                     PNELDVITVLDDTMCDGIESWAWYGRQPINVRLRRGGTLLVISNRSAEELLKVIPRKP
                     WDWTLAIVPGTPSMGGLWVYRDDGTDMRVLGAIASARPDIFGFDAISAVVKDDESLDN
                     RYLDALRSGHDEVSVYEVSAGEGKEDDYEPVKLPGWKDMREAIIVEAVKPGERNPHYR
                     SSSDRTMRPVVNFDTCIKCRQCWIDCPDECFEISEEELHPINYSYCTGCGICANVCPV
                     EDCIVMVNDLEFVEEGDKDVFPMWEDDKDAYHEWREEKIARGRIDHQAHFNADTWAK"
     gene            complement(2146..2571)
                     /locus_tag="VN178_00185"
     CDS             complement(2146..2571)
                     /locus_tag="VN178_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79407.1"
                     /translation="MDPSENSPPVPRFVFVAYYPEGPHAGPITIGLFGVQDGALLPLF
                     STREKAIDFAVRVLGTPPQNTEKWDGVELPAEDLIQFLNTDASISYVAANPPPSGDVE
                     LIPTPEFVERLEQEAGAAAGPREDFRRSLVRSFVYGDVS"
     gene            complement(2594..2803)
                     /locus_tag="VN178_00190"
     CDS             complement(2594..2803)
                     /locus_tag="VN178_00190"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HWS79408.1"
                     /translation="MARTIRCTEAQRTLDLEVDRNCDFVARAETEDEALAEFADHMQT
                     VHNWNEIFEPDLEKVRARNVHNNDV"
     gene            2976..>3272
                     /locus_tag="VN178_00195"
     CDS             2976..>3272
                     /locus_tag="VN178_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HWS79409.1"
                     /translation="MKIDKWTRRYTNAEVGPERPEVVSDSGDGEAIHQPPDGLSEAGV
                     REALRDVRDPEIGRDLVSLNMIRNIEIKGSLLTVGVALTTAGCPLKHRITTDITD"
BASE COUNT          587 a         1051 c          967 g          667 t
ORIGIN      
        1 ccttgaccct ccgcgcggcc tcgtcggtct gctttcggat ctccatcacc cagtcctcgt
       61 tcgcctgggg agcgatcgag atggggttgt cgggatggag cagccggtct cccaggtcgt
      121 acggcggcaa gtaggcgtcg acgctcttct gatccgggat cttgacgagc gcctgcgagt
      181 gtgtgatgaa cgccccgtcc acgcccaagc ccacagggaa cgagaccctc ttgtcctcgc
      241 cgacgcggta cgccataagc gtcgcgtcga agcactcctg cgcgtcctcg acccagaaga
      301 gttgccagcc caggtcgcgc acgcacatcg cgtcgttgtg ttcgaccccg aacgcaccag
      361 gatcgtccag ggcgcggttt ccgatcatgg ctacgaccgg aagacggagc ggcggggtga
      421 ccgcgagcga ctccatagcg tacatccagc ccacgcccga ggagcccacg aagacacggc
      481 tgccgacggc cgaggcgtgc ttgcagatct cgaactggga gtgctcgccc tccgcaacga
      541 tgtactcggc atcgagctcg ccgttggcga tcagcttcgc cacagcctgc atgacaccgt
      601 cgtaggggcg tatgggatag gccgccatca cgtcgacatc cgcgagacga accgcctgcg
      661 cgaccgcctg cgtgccggtc atcagttttt cggtctgctg aacccctcct gagggtatcg
      721 tgatggtcat cgtgctcctc ctacttcgcc cacgtatccg cgttgaagtg cgcctggtgg
      781 tctatcctgc ccctggcgat tttctcttca cgccactcgt ggtaggcgtc cttgtcgtct
      841 tcccacatcg ggaagacgtc cttgtcaccc tcctcgacga actcgaggtc gttgaccatc
      901 acgatacaat cctcgaccgg gcacacgttg gcgcagatac cgcacccggt acagtagctg
      961 tagttgatgg ggtgcagctc ttcctcggag atctcgaagc attcgtcagg gcagtcgatc
     1021 cagcactggc gacacttgat gcacgtgtcg aagttgacaa ccggccgcat ggtccggtct
     1081 gaggaggacc tgtagtgcgg gttgcgctcg cccggcttga ccgcctcgac gatgatcgcc
     1141 tcgcgcatgt ccttccaccc cggcagcttg accggttcgt agtcgtcctc tttgccctcg
     1201 ccggctgaga cctcgtatac gctgacctcg tcgtgcccgc tcctgagcgc gtcgaggtac
     1261 ctattgtcga ggctctcgtc atccttgacc acggcactta tagcatcaaa cccgaagatg
     1321 tcgggcctcg cgctcgctat ggcgccgagc acccgcatgt ccgttccgtc gtcgcgatag
     1381 acccagagcc cacccatgct aggcgtgcct ggtacgatcg ccagggtcca gtcccacggc
     1441 ttccgcggga tgaccttgag caactcctcg gcgctcctgt tcgagatcac caacagcgtc
     1501 ccaccccgcc tgagacgcac gttgatcggc tgccggccgt accaggccca cgactcgatc
     1561 ccatcgcaca tcgtgtcgtc caagaccgtg atgacgtcca gctcattggg ctcgtatttg
     1621 gccatgtggc cttccaacgt ctccgtgtcc ggcgcgatga ccgcgaactg cttggccggg
     1681 atgccattgc gctccggcga atcactgtag cgctggatag tagatcctaa ccagtcccgc
     1741 ttgcgggccg ccataactac atgcttgcac aggttcgccg ctaacgtctt ctggaagatg
     1801 ccccgataag tgaactcgac gctcttggcc tcgtcgcgta cctcagtaga ggtgcgtccc
     1861 tcaatggtgt cttgatcccc gggcatcgag tcgccagctt ctctgccagt cggcatcctt
     1921 tctccttccc tagtaatcgg cccccagagc caactcctac acccctcaac ttcgcccgtc
     1981 gaagctctgg cctgaccact ttaccaattt gccaagcatc ctatcagcgg caggtaacag
     2041 gatcaatggc atattgttgg gattgtatca gtttttctat gcatcctgag ctttatttgg
     2101 ctggtatgtg cggttgtatg gcgccctctt ttggggagca cgtagtcagc tgacgtctcc
     2161 gtagacgaag gagcgaacca gggaccggcg gaagtcttct ctgggaccag cagcggcccc
     2221 tgcctcctgc tcgaggcgct cgacgaattc tggggtgggg atcagctcta cgtccccgga
     2281 cggtggcggg tttgcagcga cgtaggagat gctcgcgtcg gtgttgagga actgtatgag
     2341 gtcttcggcg gggagctcga ccccgtccca tttctcggtg ttttgaggag gagtaccaag
     2401 gacgcggacg gcgaaatcta tggccttttc ccgtgtggag aagaggggca ggagggcgcc
     2461 gtcctggacg ccgaagaggc cgatggtgat gggaccggcg tgcggcccct cggggtagta
     2521 ggcgacgaat acgaacctgg gcacaggggg cgagttttcg gaaggatcca tcgagccgct
     2581 tcgtcgcgag ttgctagacg tcgttgttgt ggacgtttcg ggcgcggacc ttctcgaggt
     2641 ccggctcgaa gatctcgttc cagttgtgta ctgtttgcat gtggtccgcg aactctgcca
     2701 atgcctcgtc ctcggtctcc gcgcgggcca cgaagtcgca gttccgatcg acctcgagat
     2761 ccagcgtcct ctgggcttcc gtgcaccgga tcgtcctggc catctgcctc tcctttcctc
     2821 ctcattgatc atagcaggac ggtcaaaccc gctcaatgga ccccggcgcg gcgcccacga
     2881 cccgaaaggg gccctcgtga taacatcccc aaaaaccatg cctgagacgg cccgccgatc
     2941 ggcgacggac gtagcggagg aacggaggtc gacagatgaa aatagacaag tggacccgac
     3001 gatacaccaa cgccgaggtc ggccctgagc gaccggaggt cgtctccgac tccggagatg
     3061 gagaagcgat ccatcagcca ccggacggtc tgtcggaggc tggcgttcgg gaagcgctca
     3121 gggatgtaag ggaccccgag atagggcgcg acctcgtctc gctgaacatg atcaggaaca
     3181 tagagataaa aggttcgctc ctcaccgtcg gcgtcgccct caccaccgca ggatgtccac
     3241 taaagcacag gatcacaacc gacatcacag ac
//
LOCUS       DATMVF010000007         9103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_1235, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000007 DATMVF010000000
VERSION     DATMVF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VN178_00200"
     CDS             complement(<1..937)
                     /locus_tag="VN178_00200"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWS79410.1"
                     /translation="MNGDAAGRIVGVGRALGGRIVTNGDLEGVLDTTDEWISARTGIK
                     ARHFVAEGENAATLAVEASKRALEHAGVHGGSVDLIICGTSTAPESMPSVACLVGEEI
                     GAPGAGAMDLAAACAGFSYAASVASAMLRSGAAGRVLLIGSDAMSTIVDPTERSTAVL
                     FGDGAGAVILDAGDGDSGFVDHILGADGRGAPLGRAGHPGDDEKLFQNGREVFKFAVR
                     ILPDMVEKLLSRNALSPDEVQYVIPHQANARIIRSAARKMEVPEDRVVINLDRFGNTS
                     AASIPLSYPDIYDAMEPGKYVITVGFGFGLTWAANL"
     gene            complement(934..1947)
                     /gene="plsX"
                     /locus_tag="VN178_00205"
     CDS             complement(934..1947)
                     /gene="plsX"
                     /locus_tag="VN178_00205"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWS79411.1"
                     /translation="MRIAVDALGGDNAPREIVAGALSAAEGLPRDELLLVGKPDVIER
                     EVGSDLPPNVSLRSSGGAVGMDEEPSATLRAHPDASVAVAAEMVRDGEADALFSAGNT
                     GATVAASLLRIGRLEGCRRPAIATVLPFPSPVLLLDAGATVSCRPQDLLNFAILGGVF
                     AKSYFGLDGEARVGLINVGEEPGKGNDLAKEAYRLMDESGMRFVGNVEGRDISRGVAD
                     VLVTDGFTGNIVLKTAEGVAREILGMVRSAMTGDVLSKLAAGILRPRLIEVRDTIDPE
                     EYGGSFLLGVRGSAVIGHGNSQATGVENALLSISYVGAGLTGDLQQALSAHSLRTSDG
                     RSA"
     gene            complement(1966..2148)
                     /gene="rpmF"
                     /locus_tag="VN178_00210"
     CDS             complement(1966..2148)
                     /gene="rpmF"
                     /locus_tag="VN178_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWS79412.1"
                     /translation="MAVPKKKTSRARRNQRRAHHAITGPGLVACPNCGWPHVPHRVCP
                     ECGFYKGRTVLAVEDI"
     gene            complement(2175..2633)
                     /locus_tag="VN178_00215"
     CDS             complement(2175..2633)
                     /locus_tag="VN178_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79413.1"
                     /translation="MNHLIPLRRPGAEVGDTHEVRAEFSADPFDLYGRHHEVTKAEAE
                     VGVTRLSEGLHLDVLVMATVLTTCDRTLEPTELTLEFGDSEFFSGPNNVELSVQDWTL
                     DLARYTERALPNEVPMQVFSPGTEPVHPEGDGDEVDPRWRGLDGLFASRS"
     gene            complement(2653..3108)
                     /locus_tag="VN178_00220"
     CDS             complement(2653..3108)
                     /locus_tag="VN178_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HWS79414.1"
                     /translation="MDVLVLIDRLEELVEDARSFPGFGNTAMVDRDAAFDIIDQMRQT
                     IPEELKQARWIVKERQAMLDEARSESDRIIRSAQDEAEKITSEEEVLKRAEKRSAEIM
                     EDARRREREIRLGAEDYADEVLANLEDNLGKLLEAVQRGRTKLQGVQEQ"
     gene            complement(3130..4176)
                     /gene="coaD"
                     /locus_tag="VN178_00225"
     CDS             complement(3130..4176)
                     /gene="coaD"
                     /locus_tag="VN178_00225"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWS79415.1"
                     /translation="MRVIAGSAGGIRLAPVPRGVRPTSDRVRESLFNSLGQFFEGGKV
                     LDLYAGTGALGIEALSRGSGYAVFVERDRRARAAIHENLHRTGFVESAEVLGGEVEAV
                     LGRLAGRGDRFDLIFADPPYRIAAEVGGILHRLRSLLAPGGTVAIESGEAPVEGAGGK
                     KGVTRRYGGTFITILDRSELTMIVAVCPGSFDPVTTGHLDVIRRASKIFDHVIVAVGT
                     NRRKQTRLPAVDRARLLEKVTADMEGVSVEVMEGLLVDFARERGAQAIVKGLRAVSDF
                     ESEFEQAQLNRRMFPELETIFIMASAEHSFLSSSVVREIASLGGEVRGLVPEGVLETV
                     KQIYSGGDGNIQGS"
     gene            complement(4186..6324)
                     /gene="recG"
                     /locus_tag="VN178_00230"
     CDS             complement(4186..6324)
                     /gene="recG"
                     /locus_tag="VN178_00230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWS79416.1"
                     /translation="MASPTYRAGRASPLPARTTLTDLRARPVSELPGVGVRIEAALGD
                     LGIASLADMISHYPSRHEDLSNVKRISELRVGERATVFGTVVSTRPVGKPVRGRAPGF
                     SAQLYDGTGYIPVTVWGRGWLLNQLLPEVHVVASGEVQSRYGIQIAAKSIEVVEDPGA
                     DEGPHAGRFVPVYPVNKGIQARRMRTLIHRALDAAGRILDPLPADVLVRHGLPNLHDA
                     IHEVHFPTERALLAAAMRRLIFHELFLIQAGLAARKARLDSTEVGGRHRGDGSLLNPF
                     LKGLSFGLTGAQERVIEEVLADMRLEKPMRRLLQGDVGSGKTVVAIAALLTAVEAGGQ
                     GAIMAPTEVLAWQHHLSISAALADLPVNVVLLTGSQGVAQRREALEQIAAGGAHVVIG
                     THALIQKGVDFDDLSLVVVDEQHRFGVGQRTVFREKGTTPDTLVMTATPIPRTLSLTL
                     YGDLEVSLIDELPPGRKPVETGVVDPAERLAAYEELRKELEEGRQAYVISPLVEESEA
                     MEDVRAAEELYEELANEIFPERVVGLLHGRMRAQDKRETMGRFRAGEIEVLVATVVVE
                     VGVDVPNASAIVIEGAERFGLSQLHQLRGRVCRGLHPPKCFLVGAPKSEDSERRLKAL
                     ERYQDGFKLSEVDLAIRGEGTLFGSRQSGMPDLKVAKLLRDVDVLVEARREAFALVAG
                     DPTLKRPDHRPLRREIRELLGADVEWLFKE"
     gene            complement(6305..7120)
                     /locus_tag="VN178_00235"
     CDS             complement(6305..7120)
                     /locus_tag="VN178_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWS79417.1"
                     /translation="MTTALVTDSTTSLAPGVLERPDVRVVPLTFHFGEDETYTDKLDM
                     TNEEFYERLRDSDVFPTTAQPSPGAFVEAYEALSDYDDILVLTLSSKFSGTYDSALQA
                     AGMVDRPVQVLDAKSAEMGSGLILLEALRCIDEGGDFDEVRHVAERAIGGARVLFAVG
                     TLEYLAKSGRIGRAQRLLGTALDVRPVLEIAGGEIVPFKRTRGRKRQLATIVEEVRPA
                     AEAGRQLYFGHIGAPELLSELAEQLGVEEKLIAEIGGVVGSHVGLGAYGVAHL"
     gene            complement(7117..8691)
                     /locus_tag="VN178_00240"
     CDS             complement(7117..8691)
                     /locus_tag="VN178_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HWS79418.1"
                     /translation="MAIEVAPMVAAAHAALKAQVARINDLNVYPVPDGDTGTNMLHTL
                     GSALNETSGKSYGSPEEASKACARAALMGARGNSGVILSQMIRGACEVLAGRRSLDAE
                     AFAAGLEGARERAYASVREPVEGTMLTVIKDAALAAREAAEREDADLLSVAGVARQAA
                     HDSVRRTPELLDVLRQAGVVDAGGLGVAVVLDGVYACVSGQEIEVPESDDAAPDLEEI
                     HAQEVAWGYCTEFLVDGFDGDVEDFKERVYASGRSVLVVAQDDVVKVHVHTQDPGAAL
                     SYAGSFGRLAGVKIDDMEAQVRSREPRDRPAARLGVVAASRGDGNRALFEQMGAVVIE
                     GGQGENPSAADLAGAVQETGARAVVLLPNNKNIVPTAAQVGELVEAETYVVPTTNIAA
                     GLAVMVGYDGEGEPDEVVEEMRDISDSLRVAELTRSVREARIGDREVPEGAYIGFLDG
                     DLIAVEGGVRQTALVIARKLVDGGADVLTLLKGEGLSEGDLEEIVDGIKDLDDALEIE
                     ARDGGQPLYPLQMVAE"
     gene            8863..9087
                     /gene="rpmB"
                     /locus_tag="VN178_00245"
     CDS             8863..9087
                     /gene="rpmB"
                     /locus_tag="VN178_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWS79419.1"
                     /translation="MAKVCYSCGKKPDFGNARSHSLKATRRRRDPNLQKVRIQVGPAT
                     RREWVCTSCLKGFKVTKASIGAPSSTSQAI"
BASE COUNT         1467 a         3185 c         2817 g         1634 t
ORIGIN      
        1 acaggttcgc agcccaggtc aggccgaacc cgaagccgac ggtgatgacg tacttccccg
       61 gctccatcgc gtcgtagatg tcggggtaag agagcgggat gctcgccgcc gaagtattgc
      121 cgaacctgtc gaggttgatc acgacccggt cttccggtac ctccatcttc cgcgccgcgg
      181 aacgtattat gcgtgcattc gcctgatggg ggatcacgta ctggacctca tcgggagaga
      241 gtgcgttgcg ggagaggagc ttctcgacca tgtcgggcag tatcctgacg gcgaacttga
      301 agacctcgcg cccgttctgg aagagcttct cgtcgtcgcc gggatggccc gcgcgcccga
      361 gcggagcacc cctgccgtcg gctcccagta tgtggtcgac gaaaccggag tcgccgtcgc
      421 cggcatccag tattaccgcg cctgcgccgt ctccgaagag gacggccgtc gagcgctctg
      481 tcgggtctac gatggtgctc atggcgtccg acccgataag caagaccctg cccgcggcgc
      541 ctgagcggag catggccgaa gcgaccgaag cggcataaga gaagccggcg cacgccgcgg
      601 ccaggtccat cgcaccggct ccgggggctc ctatctcctc cccgaccagg cacgctaccg
      661 agggcatgga ctccggggcg gtggacgtac cgcagatgat gaggtcgacc gacccgccgt
      721 gtactccggc atgctcgagg gccctcttcg acgcctctac ggcgagcgtc gcggcgttct
      781 cgccttcggc gacgaagtgg cgggctttga tcccggtacg cgccgagatc cactcgtccg
      841 tggtgtcgag caccccttcc aggtcgccgt tggtgacgat cctgcccccg agtgccctgc
      901 ctactccgac gatcctgccg gcagcatcac cgttcaagcc gacctgccat ccgaagtacg
      961 gaggctatgc gcggagagcg cctgttgcag gtccccagtc aggccggcgc cgacgtacga
     1021 gatgctaagg agcgcgttct cgacacccgt agcctgcgag ttcccgtgtc cgatcacggc
     1081 cgagcccctt actccgagca ggaaggaacc gccgtactcc tccggatcta tggtgtccct
     1141 cacctcgatt agccttggac gcaggatacc cgcagcgagc ttactcagaa catcgccggt
     1201 catcgcggag cgaaccattc cgaggatctc ccgcgctacc ccctcagccg tcttgagcac
     1261 gatgttaccc gtgaagccgt ccgttacgag cacgtcggcc accccgcggc tgatgtcgcg
     1321 accctccacg ttccccacga aacgcatccc cgactcatcc atgagacgat acgcttcctt
     1381 ggcgaggtcg ttgcccttcc ccggttcctc gccgacgttg atgagcccga cccgcgcctc
     1441 cccgtccaac ccgaagtaac tctttgcgaa cacaccgcca agaatagcga aattcaagag
     1501 atcttgcggc ctgcaagaga cggtggctcc cgcgtcgagt agtaagaccg gcgaagggaa
     1561 aggaagcacc gtggcgatcg ccggtctccg gcatccttcg aggcgtccta tcctgagcag
     1621 cgacgccgcc accgtggcgc cggtgtttcc cgccgagaat aacgcgtcag cttcaccgtc
     1681 acgaaccatc tcggccgcga cggcgacgct ggcgtcagga tgggccctca gcgtcgcaga
     1741 aggctcctcg tccatcccga cggctccccc ggaggaccgc agcgagacgt tcggagggag
     1801 gtcagaaccc acctcgcgtt cgataacgtc aggctttccg acgaggagca actcgtcgcg
     1861 cggcagccct tcggccgcgg atagggcgcc ggcgacgatc tcccgcgggg cgttgtctcc
     1921 gccgagggcg tctaccgcga tacgcaccgg tgggccgatc ggggcttaga tgtcctccac
     1981 ggcgagcacc gtgcggccct tgtagaaacc gcactcgggg cagaccctgt gcggcacgtg
     2041 cggccagccg cagttcgggc aggcgacgag gcctgggccg gtgatcgcgt ggtgcgcccg
     2101 gcgctggttc ctgcgcgccc gggacgtctt cttcttggga acggccatga aaatacctcc
     2161 tggttgctac ttactcaaga tcgcgaggcg aatagaccgt cgaggccccg ccagcgcggg
     2221 tctacctcgt cgccatcacc ctcggggtgc acgggctccg tgcctggcga gaacacctgc
     2281 atcggtacct cgttcggcag ggcgcgctcc gtgtagcgcg ccagatccag ggtccagtcc
     2341 tggacgctca actcgacgtt gttcggcccc gagaagaact cggagtcgcc gaactccagc
     2401 gtgagttccg tgggctcgag cgtcctgtcg caggtcgtaa ggaccgtcgc cataaccagc
     2461 acgtcgaggt gcaatccctc ggagagacgc gtgacgccta cctctgcctc tgctttcgtc
     2521 acctcgtggt ggcgtccgta gaggtcgaaa ggatctgcgg aaaactcggc acgcacctcg
     2581 tgtgtgtctc cgacctccgc accgggtcgc cttaggggga tcaaatggtt catgaggtta
     2641 gtttactact tgctattgtt cttgaacgcc ctgcagcttg gtgcgtccgc gctgcacggc
     2701 ctcgaggagc ttgcccaggt tgtcctccaa attggcgagg acctcgtcgg cgtagtcctc
     2761 ggcgccgagg cgtatctcgc gctcccggcg acgggcgtcc tccatgatct ccgcgctccg
     2821 cttctcggcc ctcttgagga cctcttcttc cgaagttatc ttctcggcct cgtcctgggc
     2881 gctgcggatg atgcggtcgc tctcgctgcg ggcctcgtcg agcatcgcct ggcgctcctt
     2941 gacgatccaa cgggcctgct tgagctcctc gggtatggtc tggcgcatct ggtctatgat
     3001 gtcgaaggcg gcgtcccggt cgaccatcgc cgtgttgccg aaaccgggga agctccgcgc
     3061 gtcctcgacc agttcttcca acctgtctat gagtaccagt acgtccatct tctactacct
     3121 atccttcgct tacgatccct gtatattacc gtccccaccg gaatatatct gctttacggt
     3181 ctccagcacg ccctcgggta cgagcccgcg cacctctccg ccgagcgaag ctatctccct
     3241 gaccacgctc gaggagagga agctatgctc cgccgatgcc attatgaaga tggtttcgag
     3301 ctcgggaaac atccttcggt tgagctgcgc ctgctcgaac tccgattcga agtccgagac
     3361 ggccctgagg cccttcacga tcgcctgcgc accacgttct ctggcgaaat ccacgaggag
     3421 cccttccatc acctcgacgg agactccttc catgtcggcg gtcaccttct ccagaagccg
     3481 cgcccggtcg accgcgggaa ggcgggtctg cttgcggcgg ttagtgccaa cggcgacgat
     3541 cacgtggtcg aagatcttgg aagcacgcct gatgacatcg aggtgccccg tcgttacagg
     3601 gtcgaaacta cccgggcata cagccacgat cattgtcagc tcggaccgat caagtatggt
     3661 tatgaacgtg ccgccgtagc ggcgcgttac ccctttcttg cctcctgcgc cctcaaccgg
     3721 agcctcgccg ctctcgatcg cgaccgttcc tcccggcgcg agcaatgagc gtagccggtg
     3781 caagattccc ccgacctccg ccgcgattct atatggtggg tcggcgaaga tcaaatcgaa
     3841 gcggtcaccg cgcccggcga gccgccccag tacggcctcg acctcgccgc cgagtacctc
     3901 cgccgactct acgaagccgg tcctgtgcag gttctcgtgg atcgcggccc gcgcccgtct
     3961 gtcccgctcg acgaagacgg cgtacccgct cccgcggctc agggcctcta tacctagcgc
     4021 gccggtcccc gcgtagaggt cgaggacctt gccgccctcg aagaactgtc cgagcgagtt
     4081 gaacaggctc tcccgcacgc ggtcggacgt gggacgcacg cccctgggta cgggcgccag
     4141 cctgatgccc ccggccgacc cggcgatgac cctcatggcc gcaccctact ccttgaacag
     4201 ccactctacg tcggcgccga gcagctcacg gatctcgcgc cgcaggggcc tgtggtcggg
     4261 ccgctttagg gtaggatccc cggccacgag cgcgaacgcc tcgcgcctgg cctcgacgag
     4321 cacgtcgacg tcgcgcaaga gcttggcgac ctttaggtca ggcattccgc tctggcgact
     4381 accgaagagg gtgccttcgc cccttatggc gaggtcgacc tcggagagct tgaacccgtc
     4441 ctgatagcgc tccagcgcct taagacgtcg ttcggaatct tcggacttgg gagcccccac
     4501 caggaagcac ttcgggggat ggaggcccct gcacacccgg ccccttagct ggtggagttg
     4561 ggagaggccg aagcgctcgg cgccctcgat cacgatcgcg ctcgcgttcg gcacgtcgac
     4621 accgacctca acgacgacgg tcgccaccag cacctcgatc tcccccgccc ggaaccggcc
     4681 catcgtctct cgcttgtcct gtgccctcat cctgccgtgc aagaggccga ccaccctctc
     4741 ggggaagatc tcgttcgcga gctcctcgta gagctcctcg gcggcgcgta cgtcttccat
     4801 ggcctcggac tcctcgacga gcgggctgat cacgtaagcc tgccgccctt cttcgagctc
     4861 ctttctcaac tcttcgtagg ccgcgagtcg ttcggcgggg tcgaccaccc cggtctcgac
     4921 gggcttcctt cccggcggta gttcgtctat cagggagacc tcgaggtcgc cgtagagcgt
     4981 gagggagagg gtccttggta taggggtggc cgtcatgacg agggtgtccg gggtcgtccc
     5041 tttctcgcgg aacaccgtcc tctggccgac gccgaagcgg tgttgctcgt cgacgacgac
     5101 gagcgagagg tcgtcgaaat ctacgccctt ctgtatgagg gcgtgcgtcc ctatgacgac
     5161 gtgtgcccca ccggctgcta tctgctccaa ggcctctcgt ctctgcgcca cgccctgcga
     5221 gccggtgagc aagacgacgt ttacgggcag atcggccagg gccgccgaga tcgagaggtg
     5281 gtgctgccag gcgaggacct cggtcggggc cattatggcc ccctgtcctc ccgcctcgac
     5341 cgcggtaagg agcgcggcga tggcgaccac ggtcttcccg ctgcccacat cgccctgcag
     5401 gaggcggcgc atcggcttct ccagtcgcat atcggcgagg acctcctcta tgacccgctc
     5461 ctgcgccccg gtgagcccga aggagagacc cttcaggaag gggttgagca ggctcccgtc
     5521 gccccggtgc ctgccgccga cctcggtgct gtccaggcgt gccttgcggg cggcgagacc
     5581 cgcctggatc aagaacagct cgtggaagat cagacgccgc atcgccgccg caagaagcgc
     5641 cctttcggtc gggaaatgga cctcgtgtat cgcgtcgtgc aggttgggca ggccgtggcg
     5701 cacgagcacg tcggccggca aagggtcgag gatgcgcccg gccgcgtcga gcgcgcggtg
     5761 gatgagcgtc ctcatccggc gcgcctgtat gcccttgttg accgggtaga cgggaacgaa
     5821 ccgcccggcg tgcggtcctt cgtccgcccc tggatcttcg accacctcga tactcttcgc
     5881 tgctatctgt ataccgtagc ggctctggac ctcgccggaa gcgaccacgt gcacctctgg
     5941 cagcagctgg ttaaggagcc agcccctgcc ccagaccgtc acggggatgt atcccgtacc
     6001 gtcgtagagc tgcgccgaga agcccggggc gcggcccctg acgggcttgc cgacggggcg
     6061 cgtggagacc acagtgccga agaccgtggc cctctccccc acacgcaact cggagatccg
     6121 cttcacgttg gagaggtcct cgtggcgcga ggggtagtgc gagatcatgt cggccagcga
     6181 cgctatgccg aggtcgccca aggccgcctc gatgcgcact ccgacgccgg gaagttcgct
     6241 cacgggccgc gcgcgaaggt ccgtcagggt cgtccgtgcg gggaggggag acgccctgcc
     6301 ggccctatag gtgggcgacg ccataggctc ccaggcccac gtgtgacccg acgacccccc
     6361 cgatctcggc gatgagcttc tcttctaccc cgagctgttc ggccagttcg gagaggagct
     6421 cgggggcacc gatgtgcccg aagtagagct gacggccggc ctccgcggcc ggcctgacct
     6481 cctcgacgat ggtcgccagt tgccgcttgc ggccacgcgt acgtttgaaa ggaacgatct
     6541 ccccgcccgc tatctcgagc accggcctga cgtcgagtgc cgtgccgagg agacgctgcg
     6601 cccttcctat gcgcccgctc ttggccaggt actccagcgt gccgacggcg aagagcaccc
     6661 tcgccccccc gatcgcccgc tcggcgacgt gcctgacctc gtcgaagtcc ccaccctcgt
     6721 cgatacagcg cagggcctcg agcaggatca aacccgaccc catctcggcg ctcttggcgt
     6781 cgaggacctg tacgggacgg tccaccatcc ccgccgcctg cagggccgag tcgtaggtgc
     6841 cgctgaactt gctcgagagc gtgagaacca ggatgtcgtc gtagtcggag agcgcctcgt
     6901 aggcctcgac gaaggccccg ggagagggtt gcgccgtagt agggaagacg tcggagtctc
     6961 gcagccgctc gtagaactcc tcgttggtca tatctaactt gtccgtatac gtctcgtctt
     7021 cgccgaagtg gaacgtgagc gggaccaccc tgacgtcggg acgctccagc acacccgggg
     7081 cgaggctcgt ggtcgagtcg gttacgaggg ccgtggtcac tcggccacca tctgcagagg
     7141 atacaacggc tgtccgccgt ccctggcctc gatctcgagg gcgtcgtcga ggtctttgat
     7201 cccatctact atctcttcga gatctccctc agagagcccc tcgcccttga gcagcgtcaa
     7261 gacgtcggcg ccgccgtcca ctaacttcct ggcgatcacg agcgcagtct ggcggacgcc
     7321 gccctcgacg gcgatgaggt ccccgtccag gaaccctatg taggccccct cggggacctc
     7381 ccggtcgccg atgcgcgctt cgcgcacgga acgcgttagt tcggcgacgc gcaacgagtc
     7441 tgagatgtcg cgcatctcct ctaccacctc gtcgggctca ccctcaccgt cgtatccgac
     7501 catcactgcg agtcccgcgg ctatgttcgt cgtcggcacc acgtacgtct cagcctctac
     7561 cagctccccg acctgcgccg ccgtggggac gatgttcttg ttgttgggaa gcaggacgac
     7621 ggcccgggcc cccgtctctt ggaccgcccc ggcgaggtct gccgcgctcg ggttctcgcc
     7681 ctgaccgccc tcgatcacga cggcccccat ctgctcgaag agcgcgcggt tgccatcccc
     7741 gcggctcgca gcgacgacac ccagcctggc tgccggtctg tccctcggct cgcgggagcg
     7801 gacctgggcc tccatgtcgt cgatcttcac gccggccagg cggccgaagc ttcccgcgta
     7861 ggagagggcc gcgccggggt cctgcgtgtg cacgtggacc ttgaccacgt cgtcctgggc
     7921 gaccacaagt acactccggc ccgaggcgta tacgcgctcc ttgaaatcct ccacgtcgcc
     7981 gtcgaagccg tccacgagga actccgtgca gtacccccac gccacttcct gggcgtggat
     8041 ctcctccagg tccggcgcgg cgtcgtcgga ttcgggaacc tcgatctcct gcccgctaac
     8101 gcaggcgtag acgccgtcga ggaccacggc gacacccaac cccccggcgt cgacgacgcc
     8161 ggcctgccgc aagacatcga gtagctccgg ggtgcggcgc accgaatcgt gggccgcctg
     8221 tctcgccact ccggccacag agagcaggtc agcgtcctct ctttcggcag cctcgcgggc
     8281 ggccagggcc gcatccttga tgacggtgag catcgtgccc tcgaccggtt cgcgaacgga
     8341 ggcgtaggcc cgctccctag cgccttcgag ccctgcggcg aaggcctcgg cgtccaggga
     8401 tctcctgccg gccagtacct cgcacgcgcc ccggatcatc tgcgagagta tgaccccaga
     8461 gtttcccctc gcgcccatca gcgcggccct ggcgcaggct ttgctcgcct cctcggggct
     8521 accgtaggac ttgcccgacg tctcgttcaa cgcggaaccc agcgtgtgca acatgttcgt
     8581 gccggtatcc ccgtcaggca cgggatagac gttgaggtcg ttgatcctag cgacctgggc
     8641 cttgagggcc gcgtgggccg cggcaaccat gggggccacc tctatcgcca tcgcaaggaa
     8701 tccttcattg ctcctctcga ggggtttccg gcagaccaat ccccacctta ggagatcgtc
     8761 gacaatctgg tacaatcacc gcccgacatc cggattgggc ttgcgagtcg ggctttccgg
     8821 ggtccccaaa ttcgaccggt atcctagcac aggagaagtt tcgtggcgaa agtttgctat
     8881 tcgtgcggaa agaaaccaga cttcggcaac gcccgcagcc actcgctgaa ggcgacgcgc
     8941 cggcgcagag accctaacct gcagaaggtc cgtatccagg taggcccggc cacgcggcgc
     9001 gaatgggtct gcacctcgtg cctcaagggg ttcaaggtca cgaaggcctc cataggagcg
     9061 ccttcctcga cgtcgcaggc catctagccc tcaggggttc tcg
//
LOCUS       DATMVF010000008         6814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_185665, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000008 DATMVF010000000
VERSION     DATMVF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185665"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(363..2447)
                     /locus_tag="VN178_00250"
     CDS             complement(363..2447)
                     /locus_tag="VN178_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HWS79420.1"
                     /translation="MDTRIPGATENLWDAREAEGLSELEALAHRSNLLGRDRAVANYG
                     GGNTSTKAREKDHTGREVDVLWVKGSGSDLATIEAGQFTGLKLGDVLALEEREEMSDE
                     EMVAYLASCQLAPDMPRGSIETLLHAFVPYAQVDHTHPDAVNMICCSESGEGLARECF
                     GDEAVWIPYIRPGFTLSKQVGQAVRNNPDAKFVLLAKHGLVTWGDTHEESYGRTIEAI
                     NRAAEFVADRAGEPFGGRKVEPPAPGRREALLAEVLPALRGALSSGSDESSHKILRVD
                     HTSEDVLAFVCGRDSEELSQVGAACPDHLVRTKVRPLWVDFDPENEGAEELEERLRQG
                     VARYREEYEAYFSRYEEADEEMFDPNPRVVLIAGLGLVAAAKNAKEANLSRDFVYRAI
                     NVIRGAHALGGYVSLTEEESYAVEYWPLELYKLTLAPPPDELAGRVAFVTGGAGGIGS
                     AVARSLASRGACVVVCDLDEEGAGEVADGLAEPGIAARADVTEEDEVVRAYRRAVLEY
                     GGVDIVVSNAGLASSAPIEETSVEMWDKNHAVLARGYFLVAREAFRIMKEQGVGGSLI
                     FVASKNSMAAGKNASAYSSAKAAELHLARCLAEEGGPEHIRVNTVNPDAVLQGSRIWD
                     SGWREERAAAYGIQPDELEDYYRERNVLKMNVLPENVAQAVLHFASETRSSRSTGNVL
                     NVDGGVKDAYPR"
     gene            complement(2630..3847)
                     /gene="rhaI"
                     /locus_tag="VN178_00255"
     CDS             complement(2630..3847)
                     /gene="rhaI"
                     /locus_tag="VN178_00255"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HWS79421.1"
                     /translation="MESTAQRAYEVLTESLADRGVQIEAVEKRLRAQRVETPSWGYGD
                     TGTRFAVFPQTGVPRDPFEKLSDAAQVHGLTGVCPSVAVHIPWDRVEDYGELKGRAES
                     LGLHIGAVNPNLFQEEEYKLGSVSNPDPAVCRKASDHLLECVEIANQTASDVLSLWFA
                     DGTNYAGQDSFTERRHRMIEALGEVYAAMPGRMRMLLEYKPYEPAFYHTDLADWGAAL
                     TMSQRLGERAQVLVDLGHHAKEVNIEYVVALLLDEGRLGGFHFNNRKYGDDDLIVGSV
                     NPFELFLIYVELVEAEERSETRIDYMIDQTHNIEPKIEAMLLSVMNLQEAYAKALLVD
                     RAALTEARSAGDVLGAHRTLLDAYATDVRPLCARVRETLGGAPDPVAAFQQSGYAEKI
                     AEERGEGAGAGWN"
     gene            complement(3909..4940)
                     /locus_tag="VN178_00260"
     CDS             complement(3909..4940)
                     /locus_tag="VN178_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWS79422.1"
                     /translation="MFGTMRNLKVLSAALLAVLVAAAVSCGVGQQPSQGGGGGGGGPA
                     KICMMPKLVGIPYFNASEKGAEEAAKELDVELVYDGPTEAKAALQSQMIEQFIQQQCG
                     AITVAANDPNALAPAMKKADNAGIATGAWDADVAPDARDVFVNQATFQAIGYKLVDVM
                     AAQTGGKGKFLVITGSLTAPNQVAWLKEMRERMKEKYPDMSIVAVEPGEEDLQKGIDL
                     TKNYLRANPDTAGVFGITSVALPGAAEAVEQLGRDGEVPVTGLSTPNEMKPYLDAGTV
                     EKFVLWNPVDLGYLAVYVADAQINGDLPESGTFEAGRLGKVEMIAPDEVLLGPPLVFS
                     KKNIDDYDF"
     gene            complement(5003..5962)
                     /locus_tag="VN178_00265"
     CDS             complement(5003..5962)
                     /locus_tag="VN178_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79423.1"
                     /translation="MSSAVEVMRRLRFSRELVLLGVLIALVIVMSLLSPLFFTLGNLL
                     NTSRYFVEVGLMALGMTLIIITGGIDLSVGSNLALVSVAVGFTFAAGLPLPVAILFGL
                     MVGMAAGFFNSIFITFLELHPLVVTLGTFALFQGLAYGLTGARAISDFPDWFAYFGQA
                     YLLGAVPGQLIIFLVAVVIVWLILSRTSFGRYVYAIGNNEEAARFSGVPIRRVKVAIY
                     TGTGLLVAIASVIYTSRVYTARGDVGLGLELDVISAVVLGGASIYGGSGTIGGTVLGI
                     VIIAILRNGLLLAGVPSTWQLFILGVLLLVAVFLNEFFRKGEE"
     gene            complement(5959..>6814)
                     /locus_tag="VN178_00270"
     CDS             complement(5959..>6814)
                     /locus_tag="VN178_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79424.1"
                     /translation="LSVPNLESILTSVAVLGVIALAVNQVVLCGEIDISTGSMLGLCA
                     VAGGAAAQSAGGFLLPLVAAVAVGALCGAINGTLVTLGRIPSIIVTLGMLYALRGVIL
                     LVTGGAWITGVPNESRILGMGSLFGLSAPVIVLFGLFVVMEVVGRHSTWGRNVYAVGG
                     NRRAARLAGLPTNRVRFFAFVLVGVFVGIASMIYMGRAGSVQTNTGTGLELQVVAAVV
                     IGGTSISGGRGSSLAALTGAILIGVILNGLVLVGVPGIWQQAVLGALILLAVTTDVVR
                     RRLVGDGV"
BASE COUNT         1179 a         2236 c         2149 g         1250 t
ORIGIN      
        1 caagagaggt tacagaaggc tgagagggat aggatcgcac ccaggagcag acggtgcgct
       61 ggcacgttca tcttttcccc gcgtttgttt ggggtggagt gggcgataaa cactctcata
      121 cgcacacaag atccccatcg acactctctc agcctcacct accatagagt gcctttatcg
      181 gagctcggag attgccccac caatctcatc tttcccatgc cctgcaaggg cgtataccca
      241 gagcgtagtg cgcagcagcc gcttcgtcat caccaccggc ttctttcccc taggggattt
      301 tcgaagccgt ggggaggcta gaagctttag ccccgcccat ccttaccacc ctccgtcttt
      361 acttatctgg ggtatgcgtc tttcacgccg ccgtcgacgt tgaggacgtt gccggtgctc
      421 ctcgacgaac gcgtctccga ggcgaagtgg agcacggctt gggcgacgtt ctcgggcagc
      481 acgttcatct tcaggacgtt gcgctcgcgg tagtaatctt cgagctcgtc cggctgaata
      541 ccgtaggcgg cggcgcgctc ttcgcgccag cctgagtccc agattctgga cccctgcaac
      601 accgcatcgg ggttgacggt attgaccctg atgtgctcgg gtcccccctc ctcggccagg
      661 caccgcgcca ggtgcaactc ggccgccttc gccgacgagt aagccgatgc gttcttgccc
      721 gccgccatgg agttcttgga ggccacgaag atgaggctgc cgccaacgcc ctgctctttc
      781 atgatgcgga aggcctcgcg cgcgacgagg aagtagcctc ttgcgagcac ggcatggttc
      841 ttgtcccaca tctcgacgct cgtctcctcg atgggggcgc tggaggcgag gccggcgttc
      901 gatacgacga tgtcgacgcc gccgtactcc agaacggctc gccggtaggc gcgtaccacc
      961 tcgtcctcct ccgtcacatc ggccctcgcc gcgatgccag gctcggcgag gccatcagct
     1021 acctcgcccg ccccttcttc gtcgaggtcg cagaccacga cgcaggcccc gcgggaggcc
     1081 agcgacctgg ctacggcgct accgatgcca cccgccccac ccgtaacgaa ggcgaccctg
     1141 cccgcgagct cgtcgggtgg cggggctagg gtgagcttgt agagctcgag cggccagtac
     1201 tcgaccgcgt aggattcttc ttccgtgagg gagacgtatc cgccgagggc gtgggcgccg
     1261 cgtatgacgt tgatggccct gtagacgaag tccctggaga gattcgcctc tttggcgttc
     1321 ttcgcggctg cgacaagccc caatcccgct attaggacga ccctggggtt ggggtcgaac
     1381 atctcctcat ccgcctcctc gtagcgcgag aagtaggcct cgtattcctc cctgtaacgg
     1441 gcgacgccct ggcggagcct ctcctccagc tcctcggcgc cctcgttctc ggggtcgaag
     1501 tccacccaca ggggcctgac cttcgtcctg accaggtggt ccgggcaggc cgcgccgacc
     1561 tgggagagtt cctccgagtc gcgtccgcag acgaaagcca ggacgtcttc tgaggtgtgg
     1621 tccacacgca gaatcttgtg agaggattca tcggaccccg acgagagcgc cccgcgcagc
     1681 gccggcaaga cctcggcgag gagggcttct ctccgaccgg gcgccggcgg ttcgaccttc
     1741 ctgcccccaa agggttcgcc ggcccggtcg gcgacgaact cggcggcccg gtttatggcc
     1801 tcgatggtgc ggccgtagct ctcctcgtgg gtgtcgcccc aggtcacgag gccgtgcttg
     1861 gcgagcagga cgaacttcgc atcagggttg ttccttacag cctggcccac ctgcttgctg
     1921 agggtaaagc ccggtcttat gtaggggatc cagaccgcct catccccaaa gcattcacgc
     1981 gcgagcccct cgccgctttc cgagcagcag atcatgttga ccgcgtcggg atgcgtgtgg
     2041 tcgacctgcg cgtaaggaac gaaagcgtgc aggagcgtct ctatggaacc cctcggcatg
     2101 tcgggcgcga gctggcaact ggccaggtag gcgaccatct cctcgtcgct catctcctcc
     2161 cgctcctcga gcgcgagtac atcgcccagc ttgagtcccg taaactgccc ggcctcgatg
     2221 gtcgccaggt cggatcccga ccctttcacc cacagtacgt cgacctcccg cccggtatgg
     2281 tccttctccc tggccttggt cgaagtgttg ccgcccccat agttggcgac ggcgcggtcc
     2341 ctccccagca ggttggaacg gtgggccaga gcttcgagct cggagagacc ctcggcctct
     2401 cgcgcatccc acaggttctc cgtcgcgcct ggaattcgcg tatccatgtg tgtggcctcc
     2461 cttcggtcgt cttagcattt cagcatttca gcacgctcca gcctccggct gtcgcttgtc
     2521 agcatttaag ctttcgtgtg cacaagccct gactggctga cgaggcggca aagccgccgg
     2581 gctgaagtgc tgacgagcga aggcgctagc ctgagcgtgc tgatttgctt cagttccatc
     2641 ctgctcccgc cccttcgccg cgctcctcgg cgatcttttc ggcgtatcca ctctgctgga
     2701 aggcggctac ggggtctggg gcgccgccca gtgtctcgcg gactctggcg cagagcgggc
     2761 ggacgtccgt ggcgtaggcg tccaggaggg tgcggtgggc gccgagtacg tcgccggcgc
     2821 tccgcgcttc cgtgagggct gcgcggtcta cgaggagggc ttttgcgtaa gcttcctgca
     2881 ggttcattac cgagaggagc atggcttcta tcttgggctc gatgttgtgc gtctggtcga
     2941 tcatgtagtc tatgcgggtc tcggagcgct cctcggcctc gacgagctcg acgtagatca
     3001 agaacagctc gaatgggttg acgctgccca cgatcaggtc gtcgtcgccg tacttcctgt
     3061 tgttgaagtg gaaacccccg agacgcccct cgtcgaggag caacgccacg acgtactcga
     3121 tgttgacctc tttggcgtgg tggccgaggt cgaccaggac ctgcgcccgc tcgccaagtc
     3181 tctggctcat agtgagggcc gcgccccagt cggcgaggtc tgtgtggtag aaggccggct
     3241 cgtagggctt gtactcgagg agcatcctca tgcgcccggg catggcggcg tagacctcgc
     3301 ctagagcctc gatcatcctg tggcggcgtt ccgtaaagga gtcctggccc gcgtagttcg
     3361 tgccgtcggc gaaccacaaa gagaggacgt cggaggctgt ctgattcgcg atctcgacgc
     3421 attcgaggag gtgatccgag gccttcctgc acaccgccgg gtccgggttg ctgacgctgc
     3481 cgagcttgta ctcttcttcc tggaagaggt tcgggttgac ggcccctatg tgcagcccca
     3541 gagactcggc gcgacccttc aattcgccgt agtcttccac cctatcccag ggtatatgga
     3601 cggccaccga ggggcatacg ccggttagtc cgtgcacctg tgcagcgtcg gacagcttct
     3661 cgaacgggtc gcggggcact cctgtctgcg ggaagacagc gaagcgcgtg cccgtgtcgc
     3721 cgtagcccca cgagggggtc tccaccctct gggcacgcag ccgcttctct accgcttcta
     3781 tctggactcc gcggtcggcg agggactctg tgagaacttc gtaagctcgt tgggctgtgg
     3841 actccaaggt tccctcctct attgggctag ggcgccccct ttgtacggtg cgccctggcc
     3901 agcaattact agaagtcgta gtcgtcgata ttcttcttcg agaacaccag cggtggtccc
     3961 agaaggacct cgtcgggggc gatcatctcg accttaccga ggcggcccgc ctcgaatgtt
     4021 cctgactcgg ggaggtcgcc attgatctgg gcgtccgcga cgtagacggc caggtagccg
     4081 aggtccacgg ggttccagag gacgaacttc tccacggtcc ccgcatcgag gtatggtttc
     4141 atctcgttag gggtggagag tcctgtcacc ggcacctcgc catcgcggcc gagttgttcg
     4201 acggcctcgg ccgcgccagg cagggccacg gaggttatgc cgaaaacgcc ggccgtgtcc
     4261 gggttggccc tgaggtagtt cttggtcagg tcgatgccct tctggagatc ttcctcaccc
     4321 ggttcgacgg cgacgataga catgtcgggg tacttctcct tcatgcgctc gcgcatctcc
     4381 ttgagccagg cgacctggtt gggggcggtc agtgaaccgg tgatgaccag gaacttgccc
     4441 ttgccgccgg tctgcgcggc catgacgtcg accagcttgt agccgatagc ctggaaagtt
     4501 gcctggttga cgaaaacgtc cctggcgtcg ggggccacat cggcgtccca ggcgcccgtg
     4561 gcgatcccgg cgttgtcggc cttcttcatg gctggggcca gggcgttcgg gtcgttggcg
     4621 gccaccgtga tggcgccgca ctgctgctgg atgaactgct cgatcatctg cgactggagc
     4681 gcggccttgg cctcggtcgg gccgtcatag accagctcga cgtccagttc cttcgcggcc
     4741 tcttcggccc ctttctcaga agcgttgaag taagggatgc cgaccagctt gggcatcatg
     4801 cagatcttgg ccggtccacc gcccccacct ccgccgccct gcgacggctg ctggcccacc
     4861 ccacacgaga cggccgcggc gacaagcacc gcgagcaggg ccgccgagag taccttcaga
     4921 ttcctcatcg taccgaacac gctactcctc cttcccgaag atttgccttt agagaccttt
     4981 acccgctcta gccgtacatg tactactcct cacctttgcg gaagaactcg ttcaggaaca
     5041 cggccacgag gagcaagacg ccgaggatga agagctgcca ggtgctcgga actccggcca
     5101 ggagcaggcc gttgcgcagt atcgcgatga tgacgatgcc gagtacggtt ccgcctatgg
     5161 ttcccgagcc gccgtagatg ctcgcccctc ccagcacgac ggcggagatc acatccagct
     5221 ccagccccag gccgacgtcg cccctggccg tatagaccct cgaggtgtag atgacggagg
     5281 cgatcgccac gagcagcccg gtaccagtgt agatggccac cttgaccctg cggatgggca
     5341 cgccggagaa acgcgccgcc tcttcgttgt tcccaatggc gtagacgtag cgcccgaagc
     5401 tggtgcgcga gagtatgagc cacacgatca ctacggcgac caggaagata atgagctggc
     5461 ctggcaccgc gccgagcagg tacgcctggc cgaagtaggc gaaccagtca gggaagtccg
     5521 agatggcccg ggcgcccgtc aggccgtagg cgagtccctg gaagagggcg aaggtcccca
     5581 gggtaacgac gagggggtgc aactccagaa acgttatgaa gatgctgttg aagaatccgg
     5641 cggccatacc caccatcagg ccgaacaaga tggcgacggg cagagggaga cccgccgcaa
     5701 aggtgaatcc gacggccacg gagaccagcg ccaggttcga cccgaccgag aggtctatcc
     5761 ctcccgtgat gatgatcagg gtcatcccca gcgccatgag gccaacttcg acgaagtagc
     5821 gtgaggtgtt gagcaggttg ccgagggtga agaagagggg cgagagcagg ctcatcacga
     5881 tgaccagcgc gatcaggacg cccagcaaga ccaactcgcg ggagaaccgc aggcgccgca
     5941 tgacctcgac agccgagctc ataccccgtc ccccacgagc cgtctgcgca cgacgtcggt
     6001 ggtcacggcg agcaggatca gggccccgag cacggcctgc tgccagatgc cgggcacccc
     6061 gacgagcacc aggccgttca ggatgacgcc tatgaggatc gcaccggtca gggccgcgag
     6121 tgaggagccc cgtcctcccg agatgctcgt cccgcctata acgacggcgg cgacgacctg
     6181 aagctcgagg ccggtacccg tgttggtctg caccgagccc gcgcgcccca tgtagatcat
     6241 ggaggcgatg ccgacgaaga cgccgacgag cacgaaggcg aaaaaccgga cccggttcgt
     6301 gggcagcccg gccaaacggg ccgcccgccg gttgccaccg acggcgtaga cgttccggcc
     6361 ccaggtggaa tggcgtccga cgacctccat cacgacgaaa aggccgaaga ggactatcac
     6421 gggggcgctc aagccgaaca agcttcccat gccaagtatc ctggactcgt tggggacgcc
     6481 ggtgatccag gctccgcctg ttaccagtaa gatcacaccc ctgagggcgt acagcatccc
     6541 gagcgtcacg atgatggaag ggatacgccc cagcgtgacg agggtaccgt tgatcgcccc
     6601 gcaaagggcg ccgacggcga cggcggccac aagcggcagc aaaaaccccc cggcactctg
     6661 cgccgcggcg cccccggcca ccgcgcacag acccagcatc gagcccgtcg agatgtcgat
     6721 ctccccgcag aggacgacct ggtttaccgc cagggctatg accccgagca cggccacgga
     6781 ggtcaggatg gactcgaggt tgggcacgct gagg
//
LOCUS       DATMVF010000009         6840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_322626, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000009 DATMVF010000000
VERSION     DATMVF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322626"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VN178_00275"
     CDS             <1..243
                     /locus_tag="VN178_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HWS79425.1"
                     /translation="AFSGDEEGWGYSTVSDADEFLERYESLIDALLRSEPVQGFCYTQ
                     LTDVEQETNGLLTYDRRPKASLARINEITACMRPQT"
     gene            521..1864
                     /locus_tag="VN178_00280"
     CDS             521..1864
                     /locus_tag="VN178_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS79426.1"
                     /translation="MSGRNETSRRRLAQPISRRRFLYGAATALGGLAVTGCGAVSIGG
                     TGRSVDFWNLFGGGDGARLGAMESTFRKDNPDIQLESTTLAWGAPYYTKLSMSAVGGR
                     PPEVAILHQTHLASYAPAGLLEPLDPQLLSEFDIGPDNFLPTILESSKYEGEIYTIPL
                     DTHPFIQYYNTDICKKAGLLGPDDELKPLEGPDAVIEALKKAKEVTGALGLSFYAADS
                     AGPWRLFYTLYGQLEGSPIISPDTREVTVDEEKSLQALEFMVALSQDAEVVPVDLDYG
                     GSVAEFLSGNAGFHWNGDWEVTTYQDADLPFSMVPFPNVFGENHISQADRHTFVIPKQ
                     PTKDPRAMEPAFQFISSMLKNSLVWAKGGHIPAYLPVAESEEYRKLSPQSNYVGVADN
                     VVLDPEAWFSGSGSTMEQRASATFQTAMAGIITPKQAFAELRTMLQEFVDTPKPV"
     gene            1900..2826
                     /locus_tag="VN178_00285"
     CDS             1900..2826
                     /locus_tag="VN178_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS79427.1"
                     /translation="MAAVGSREVKHSTKPAAQRRIDLNGYLFVLPFLVFYALFLIWPV
                     ILGMRMSFFNWSLVGTGAREFLGLTNFRELFADPDFWSSLWHSILFTLLVVPTIVVFA
                     LGLALLANRAIPARWFFRISFFAPYLLPSSVIALIWIWMYQPGFGLINSYLTRIGLPE
                     VSWLTDPQVAMISVVIATVWWQLGFSFVLYLAGLQEIPKELYDAAAVDGASPLVRTRY
                     ITIPLLRRTTILVVALALLANLNVFDQIYIMSTSGGLDYASTRSLIVYVYEQGFTLYR
                     VGYAAAMTYVFFVLVLAVSIGSFVLNNRRRDV"
     gene            2826..3701
                     /locus_tag="VN178_00290"
     CDS             2826..3701
                     /locus_tag="VN178_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS79428.1"
                     /translation="MVVEAKAPETTREAGMQRRDRFQSWLAFGVLIVAAILWLIPLLW
                     AVNTALKPEPETTTVPITWIPEGGFTLESFWQVLTAGGLVRWYFNSALTSSIITAAVL
                     LLGSLAAFGLSRVPFRGRTVVFWVIIAGLIVPPQILIVPLFMEMDALGFVDTYQGIIL
                     PQIASPLAVFIFKQFFDGIPAELEEAAVVDGASRLRVYWQVWMPLARPAIAAVGIFTF
                     VLSWNNFLWPFVVVTNTDMMTIPVGLATVQSSYGLRYADIMASAVLGGLPLVIVFLFF
                     QRQIVQGIASTGLKG"
     gene            3712..5226
                     /locus_tag="VN178_00295"
     CDS             3712..5226
                     /locus_tag="VN178_00295"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HWS79429.1"
                     /translation="MHSARIKVDPDFRIGEVDPRLYGSFIEHLGRAVYGGIYEPEHPT
                     ADDMGFRGDVLELVRELRVPIVRYPGGNFVSGYDWEDGVGPKEARPRRLDLAWRTVET
                     NEVGTDEFAAWARRAGAEVMMAVNLGTRGVDAARDLVEYCNHSSGSRYSDLRRSHGAK
                     EPHGIKVWCLGNEMDGPWQIGQKTPQEYGRLACEAAKAMKWVDPSIELVACGSSYAAM
                     PTFAEWERTVLEHTYDHVEYVSLHTYYRNPEDDRPTFLARSLNMDAFIESVIATCDFV
                     RAKKRSKKTIDLSFDEWNVWFHSIEADRELEPWSVAPPQLEDVYTFEDALVVGCMIIS
                     LLKHADRIKIACLAQLVNVIAPIMTENGGPAWRQTIFWPFLHASTYGRGTALNLAVES
                     PRYDNDEFGEVPLLEAVATLEDDERSLTVFAVNRGEEDPLPLEGDLRSFAGYEVVEHL
                     VLEHEDPKATNTAKHPDNVTPHKGGDATIQDGRLTVLLPRLSWNVIRLSRSPTG"
     gene            5353..5598
                     /locus_tag="VN178_00300"
     CDS             5353..5598
                     /locus_tag="VN178_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWS79430.1"
                     /translation="MSALHERRIAVPEDCAVVGCDDIPIATHAIPPLTTVHIPFFETG
                     ETAVRLLLGRISGKRSDHGPSCCQSGSCDASRAAPEK"
     gene            5637..5858
                     /locus_tag="VN178_00305"
     CDS             5637..5858
                     /locus_tag="VN178_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79431.1"
                     /translation="MARIKVDPDRTVGTLDRRVFGGFIEHLGRCIYGGVFDEGSPLSD
                     ERGYLEAKGDSLEYCFPACSFTVLLVGLA"
     gene            5888..>6840
                     /locus_tag="VN178_00310"
     CDS             5888..>6840
                     /locus_tag="VN178_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HWS79432.1"
                     /translation="MVDTSGSPHAHLRTLPLEAINLVDDFWEPRRRINREVTLPSQYR
                     HLEETGRLDNFRRASGKIGGEYQGIYFNDSDVYKWLEAASWTLATDPDPDLERMVDDA
                     ITEIADAQRPDGYLNTYFTFEKAGERWTDFDLHEMYCAGHLFQAAVANFRATGSERLL
                     DVATRFADHICDTFGPEEEKRHAVDGHEEVEMALVELFRVTGEKRYLEGARYFIDARG
                     HGLLGRPYGQHDPSYSQDHEPLRNQSEVVGHAVRAMYHYSGAADVYAETGELELMEAL
                     RRLWMNMTTRRMYVSGGLGSRYEGEAFGKDFELPNERAYAET"
BASE COUNT         1291 a         2131 c         2145 g         1273 t
ORIGIN      
        1 gccttctccg gcgatgagga gggctggggt tacagtactg tgtccgacgc ggacgaattc
       61 ctcgaacgct acgaatcgct catcgacgcc ctcctgcgga gcgagcccgt gcagggcttc
      121 tgctacacgc agctcacgga tgtggagcag gagacaaacg gcctcctcac ctacgaccgc
      181 aggcctaagg cgtccctcgc ccgcataaac gagataacag cttgtatgag gccgcaaaca
      241 taagggttcg tgagcggcaa ggttgagaga atccggtatg tatcctgtca agtgtgtaag
      301 gatggtcgtg tggtcccaaa ccttactgct ccttgaccgg agtagacaat agtacctcac
      361 cgagcggagc ttgcgtgtcg catattgcag caagggtctc gaagctgtaa ggtgagcatc
      421 aggacgggga agctctccta agagcggtat gtatgtaaaa agagcgatcg acgaccggtg
      481 gttaacgaat cgagcgaagg gtcgcgaaag gggaaagggc atgtccgggc gaaacgagac
      541 gtcgaggcga cggctggcgc aacccataag ccgccgtcgc ttcctgtacg gtgcggcgac
      601 ggcgctaggt gggttagctg tgacgggctg cggcgccgtc tccatcggcg gtacggggcg
      661 cagcgtggat ttctggaacc tgttcggcgg cggggatggc gcgcggctgg gcgcgatgga
      721 gagcaccttc cgaaaggata atcccgacat acagctcgag tccaccacgc tcgcctgggg
      781 tgccccttac tacaccaagc tctcgatgtc cgcggtgggg ggccgtcccc ccgaggtagc
      841 catcttgcac cagacgcacc tggcctcgta cgccccggcg ggtctgctcg agcctctcga
      901 cccgcagctg ctctcggagt tcgacatcgg acccgacaat ttcttgccca ccatcctgga
      961 gagctccaag tacgaaggcg agatctacac catccccctg gatacgcatc cgttcataca
     1021 gtactacaac accgacatct gcaagaaggc cgggctcctc gggccggatg acgagctcaa
     1081 gccactcgag ggcccggacg cggtcatcga ggcgctcaag aaggccaagg aggttacggg
     1141 ggctctgggg ctctcattct acgcggctga ttcggcgggg ccgtggcgcc tcttctacac
     1201 cctctacggg cagctcgaag gtagcccgat tatatctccg gacacgcggg aagtgacggt
     1261 ggacgaggag aaatccctgc aggcgctgga gttcatggtc gcgctctcgc aggacgccga
     1321 ggtcgtcccc gtcgacctgg actacggcgg ctcggtggcg gagttcctga gcggcaatgc
     1381 ggggttccac tggaacggag actgggaggt caccacgtac caggatgcgg acctgccctt
     1441 cagtatggtc ccgtttccga acgtcttcgg cgagaatcac atctctcagg cagaccggca
     1501 caccttcgtc atccccaagc agcccacaaa ggacccccgc gccatggagc cagcgttcca
     1561 gttcatcagc tccatgctga agaacagctt ggtctgggcc aagggcgggc acatcccggc
     1621 ctacctgcct gttgcagaga gcgaggagta cagaaaactg agtccgcaat ccaactacgt
     1681 gggggtggcc gacaacgtcg tgttggatcc cgaagcctgg ttcagcgggt caggttctac
     1741 gatggagcag cgagcatcgg ctaccttcca gacggcgatg gccggcataa tcacgccgaa
     1801 gcaagccttc gccgagctcc gaacaatgct tcaggagttc gtcgacacgc ccaaaccggt
     1861 gtaagggcga caaatgttca ttgtagagag gagcaagaca tggcggcggt agggtccagg
     1921 gaggtcaagc acagtaccaa accggcggcg cagcggcgga tagatctgaa cgggtacctc
     1981 ttcgtgctgc cgttcctggt attctacgcc ctcttcctga tatggcccgt aatcctgggt
     2041 atgaggatga gcttcttcaa ctggagcctc gtgggcaccg gtgccaggga attcctcggg
     2101 ctcaccaact tccgggagct cttcgccgat ccggacttct ggagctcgct gtggcactcg
     2161 attctattca cgctgctcgt cgtccctacc atagtggtct tcgccctcgg gctggcgctg
     2221 ctcgccaacc gggcgatccc cgcgcggtgg tttttcagga tctccttctt cgcgccgtac
     2281 ctcctgccct cctccgtgat agccctgatc tggatctgga tgtaccagcc ggggttcggc
     2341 ctgatcaaca gctacctcac ccgcatcggc ctgccggagg tctcatggct caccgacccg
     2401 caggtcgcca tgatctcggt ggttatcgcc accgtgtggt ggcagttggg tttcagtttc
     2461 gtgctctacc tggccgggct gcaggagatc ccgaaagaac tctacgacgc tgccgccgtg
     2521 gacggagcca gtcccctggt ccggacacgg tacatcacga taccgctcct gaggcgcacg
     2581 accatcttgg tcgtcgcctt ggcgcttctc gcgaacctca acgtcttcga ccagatctac
     2641 atcatgagca cctcgggggg cctcgactac gcgtccacgc gctcgctcat cgtgtacgtc
     2701 tacgagcagg gcttcaccct gtaccgggtg ggctacgccg cggccatgac gtatgtgttc
     2761 ttcgtgctcg tgctggcggt gagcatcggg agtttcgtgc tgaacaaccg taggagggac
     2821 gtctgatggt cgttgaagcc aaagccccgg agacgacaag agaagccggc atgcagcgcc
     2881 gggacaggtt ccagagctgg ctggctttcg gggtgctgat agtggcggcg atcctctggc
     2941 tcataccgct tctgtgggcc gtgaatacgg cgctcaagcc cgagcccgag accacgaccg
     3001 tcccgatcac ctggattccg gagggaggtt tcaccctcga gtccttctgg caggttctga
     3061 ccgccggggg tctggtgcgt tggtacttta acagcgcgtt gacctcctcg atcatcaccg
     3121 ccgccgttct gctactcggc agcctcgcgg ccttcgggct ctcgcgcgtt cccttccggg
     3181 gacggaccgt cgtgttctgg gtgataatcg ccgggttgat cgttcccccg cagatactca
     3241 tcgtacccct gttcatggag atggatgccc tggggttcgt agatacctac cagggtatca
     3301 tcctgcctca gatcgcctcg cctctggctg tgttcatctt caaacagttc ttcgacggca
     3361 tcccggccga gcttgaggag gccgcggtcg tggacggagc gagccgcttg agggtctact
     3421 ggcaagtctg gatgccgctc gcaagaccag cgatcgccgc ggtcgggatc ttcaccttcg
     3481 ttctctcgtg gaacaacttc ctctggccgt ttgtcgtagt gaccaacacg gacatgatga
     3541 ccatccccgt gggcctggcg acggtgcaga gctcctacgg cctccgttac gccgacataa
     3601 tggcctctgc cgtgctcggc gggctgccgc tggtgatcgt cttcctcttc ttccagcgcc
     3661 agatcgtcca gggcatcgcc agcacgggcc tgaaaggcta ggctccgtag catgcacagc
     3721 gccaggatca aggtggaccc cgatttccgg atcggcgagg tggatccgcg cctctatggc
     3781 tcctttatcg agcacctcgg acgcgccgtc tacggcggca tctatgagcc cgaacacccc
     3841 accgccgacg acatggggtt ccggggtgac gtcctcgaac tcgtccggga gctaagggtc
     3901 cccatcgttc gctaccccgg gggcaacttc gtctccggct acgactggga ggatggcgtg
     3961 ggtccgaagg aggcgcgccc gcggcgcctg gatctcgcct ggaggaccgt ggagaccaac
     4021 gaggtcggca ccgacgagtt cgctgcgtgg gcgcggcgcg ccggtgccga ggtgatgatg
     4081 gccgtcaacc tcggcacccg cggcgtggat gccgcgaggg acctcgtcga gtactgcaac
     4141 cactcctcgg gcagccgcta cagcgacctg agacgatctc acggtgcaaa agagccgcac
     4201 gggatcaagg tgtggtgcct gggcaacgag atggacggtc cgtggcagat cggtcaaaag
     4261 acaccccagg agtatggccg cctggcatgc gaggcggcga aggctatgaa gtgggtggat
     4321 ccctccatag aactcgtcgc ctgcggcagc tcctacgccg cgatgccgac cttcgccgaa
     4381 tgggagagga cggtcctcga gcacacctac gaccacgtcg aatacgtctc gctgcacacc
     4441 tactacagga acccggagga cgaccgtcct accttcctgg cgcggtcgct caacatggac
     4501 gcgttcatag aatctgtgat cgcgacctgc gacttcgtca gggccaagaa gcgtagcaag
     4561 aagaccatag acctctcctt cgacgagtgg aacgtctggt tccactccat tgaggcggac
     4621 agggagttgg agccttggtc ggtagctccc ccgcagctcg aagacgtgta caccttcgag
     4681 gacgctctgg tcgtggggtg catgattatc tccctgctca agcacgcgga caggataaag
     4741 atcgcctgcc tcgcccagct cgtgaacgtc atcgccccta tcatgaccga gaacggcggc
     4801 cccgcctggc gccagaccat tttctggccc tttttgcacg cttccaccta cggtcgaggg
     4861 acggcgctga acctggctgt cgagtcgcca cgctacgaca acgacgagtt tggggaggtc
     4921 ccgctactcg aagcggtcgc caccctggaa gacgacgaga gaagcctcac cgtcttcgcg
     4981 gtcaaccggg gcgaggaaga ccccctcccg ctggagggtg acttaaggag tttcgcgggc
     5041 tatgaggtgg tcgagcacct cgtcctcgag cacgaggatc cgaaggcgac gaacacggcc
     5101 aagcaccccg acaacgtcac gccgcacaaa gggggcgacg ccaccattca ggatggtagg
     5161 ttgacagtcc tcctgccccg gctctcgtgg aacgtgatcc gcttgtcgag gtcacctacc
     5221 ggctgaccgg tgcgaggggt ggtttctaga gaagaccgcg ggcaagcgag gccggtgaac
     5281 gagcgtgtag gcccgagcag aaagaactac gctggaacga cctgtactat cacttggcga
     5341 aagggttgta gcttgagcgc gctgcatgag cggcggatcg cggtgcctga ggactgcgcg
     5401 gtggtcgggt gcgacgatat ccccatcgcc acgcacgcga ttccgccgct caccacagtg
     5461 cacataccgt tcttcgagac gggggagacc gccgtgcgcc tcctgctggg ccggatctcg
     5521 gggaaaagat cagaccacgg cccgtcctgc tgccagtccg gctcgtgcga cgcgtctcgt
     5581 gcggcgccgg agaagtaatg acgaggaact actcacccat cggagaggag tagagggtgg
     5641 cgaggatcaa ggttgacccc gaccgcacgg tcggcacgct ggaccggagg gtcttcggcg
     5701 gctttattga gcacctggga cgctgcatct acggcggtgt cttcgacgag ggctcccccc
     5761 tgagcgacga gcgcggctac ctggaggcaa agggagattc ccttgaatac tgtttcccgg
     5821 cctgctcgtt cacggtcctg cttgtgggtc tggcatgagc acgctcgact cggcgcaacg
     5881 agcgcacgtg gtagatactt ccggcagtcc ccacgcgcac ctgcgaaccc tgccgctaga
     5941 ggccatcaac ctcgtcgacg acttctggga gcccaggcga cgtatcaatc gagaggtcac
     6001 gttgccttct cagtacaggc acctcgaaga gactggccgc ctggacaact tccgccgcgc
     6061 ctcgggcaag atcggcggcg agtaccaggg gatctacttc aacgactcgg atgtgtacaa
     6121 gtggctcgaa gcggcatcct ggacgctcgc caccgaccct gaccccgacc tggagcggat
     6181 ggtcgacgac gccataaccg agattgccga cgcccagagg cctgacggat acctgaacac
     6241 gtacttcacg ttcgagaaag ccggcgagcg ctggaccgac ttcgacctgc acgagatgta
     6301 ctgcgcgggc catctctttc aggccgccgt cgccaacttc agggctaccg gatctgagcg
     6361 tctcctcgac gtggccacgc gcttcgccga ccatatctgt gataccttcg ggcctgaaga
     6421 ggaaaaacgt cacgctgtcg acggccacga agaggtcgag atggccctcg tcgagctgtt
     6481 ccgggtcacg ggtgagaagc gctatctcga aggcgcgcgg tacttcatcg acgctcgcgg
     6541 gcacggctta ctcgggcgtc cctacgggca acacgacccg tcctacagcc aggaccacga
     6601 gccactgcgc aaccagagcg aggtcgttgg ccacgccgta cgtgccatgt atcactactc
     6661 cggcgccgcg gacgtgtacg ctgagacggg tgaacttgaa ctcatggaag ccttgaggcg
     6721 tctgtggatg aacatgacca cacgtcgcat gtacgttagc ggcggtctcg gctcgcgcta
     6781 tgagggcgag gcgttcggta aggacttcga gttacccaac gagcgagcct atgcggagac
//
LOCUS       DATMVF010000010        12944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_94055, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000010 DATMVF010000000
VERSION     DATMVF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94055"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..761
                     /gene="ribA"
                     /locus_tag="VN178_00315"
     CDS             114..761
                     /gene="ribA"
                     /locus_tag="VN178_00315"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HWS79433.1"
                     /translation="MMSVTRRVAQAGLPTRYGTFEMFVYGTPDHKEHVALTLGAIDDG
                     GPVLVRAHSECLTGDVFGSSRCDCGEQLGESLRFLQEQGRGVLLYLRQEGRGIGLTRK
                     VSAYALQEQGFDTVEANLALGLPEDMRDYRVAAEMLLDLGVRRVCLLTNNPAKIEGIE
                     GYGVEVVERIPLGITPNPSNLEYLRTKREKMGHLLPMAEPLEVACEGSPWRKWRG"
     gene            754..1620
                     /locus_tag="VN178_00320"
     CDS             754..1620
                     /locus_tag="VN178_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWS79434.1"
                     /translation="MDEREYRVRYGIVMSNLDDYADPRQAVSLARAAEEAGWEGFFVW
                     DHLGFVWGAPSCDPWGVLSAIAVSTTRLKLGTAITPLARRRPHIVANTLASLDLLSGG
                     RVIFGAGLGGVAKEFTAFGEPGDAKVRARMLDEGLTVVDGLWSGEPLTHHGQHYDVEN
                     VSLAPLQRPRIPIWIGGEGAPALRRAARWDGWLAPATSPDGMAKDPERIAEMIAEIQH
                     HRRADTPFDVAVDGYSDPGDPALPRAYEAAGATWWLESIHGMRGSVEETMARVEAGPP
                     EEGGDDASDSRR"
     gene            1595..2542
                     /locus_tag="VN178_00325"
     CDS             1595..2542
                     /locus_tag="VN178_00325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWS79435.1"
                     /translation="MMLAIAEDEFGGPVSLMTLPVPEIGAGEVLIRVRAAGVNPFDWK
                     VADGVLEDEKEHRFPLILGFDAAGVVERVGSDVRGLSEGDKVYGYLSKPVMGQGAYAE
                     YVGAPADIVARKQEAVSFAEAAALPMPGLTAMDLVDAVDLEEGETVLIVGATGGVGSY
                     ATQLAAGRGARVIATARQENKVFARELGAVQTIDHTREDLAKSVRAAHPDGIDAVIDV
                     VSEGEALDRIARLLKEGGRLASSVYAADIEGLARRSIQATNVSMQPDARRLEELSRMV
                     DAGDLSVRLEHAFPLERAPEALEESRAGHVRGKIVLLVD"
     gene            2631..3956
                     /locus_tag="VN178_00330"
     CDS             2631..3956
                     /locus_tag="VN178_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HWS79436.1"
                     /translation="MRYNDMGDWTRAAPNLYPHQWSWDSAFIAIGLAHLDTRRAAKEL
                     LALFSHQWRTGKIPHIVFNQEAPPESYFPGAEHWACASTSPDAPPAPPYTSCLCQPPV
                     HAIAALRIFEVAQRNRGEDTRAAQAFLQEAYPSLLRWHRYLSTYRDPEGSGLVTIYHP
                     WESGTDNSPRWDTVLAAVEVGDLPPYPRYDLQHVANRSQRPTDADYDRYLWLVKLIKK
                     ASCDERSIYRYHPFLVKDVLFSAILVAANEALLEIARLVGAQEEERALITDWIERGRR
                     GLAARWDPEVGLCLDHDLRSGRPLRVRTVAGFSLLIAGGQSPEQVDALMATLDSPAFL
                     GHPWLRWSLPPSTSPLQEGFHRSSYWRGPTWPVVNWLLWWSLLRAGKPMWAERLRQAS
                     LQQIASSGFGEYFDPYTGERLGATDQSWTAAVALDWLANGPRRVERKVA"
     gene            complement(3993..5177)
                     /locus_tag="VN178_00335"
     CDS             complement(3993..5177)
                     /locus_tag="VN178_00335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWS79437.1"
                     /translation="MGHRHQLVSMSRLLAALGSLVWLGLCLQWLLGIRKIPVLEDVLG
                     TDLVDRNPALSVILAARDEERSLGESLPSMLAQDYSGMIEVIAVNDRSTDRTGEILEE
                     LATKHPDGLRILDVESLPDGWLGKTYALYAGAIQARGEWLLFTDADVIFTPSCTDNAV
                     RYATTNGLDHLTLPPEIVCRSVLLRSFVAAFTLVFEMTQRPWRVGDPQAQEHVGVGAF
                     NLIRKDAYERSGTHRAIRMRPDDDMKLAKILKRHGFRQGVAYGVGLISVEWHQALPDA
                     VRGLSKSIFSGLDYRIGATVAGVLMLLLTNVLPVFGLFSRKTTGTLCRMNILSTFLVY
                     VYRARHFGDKTPWWYAVLHPFGICVFLYAVLRSASTTIINRGIEWRGTRYPLKDLKDN
                     AI"
     gene            5452..6366
                     /locus_tag="VN178_00340"
     CDS             5452..6366
                     /locus_tag="VN178_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS79438.1"
                     /translation="MVTPHDNIIRPNRLLLATDGSEDAVLAARVATDLSRRTGARLHV
                     VHAWYHLVKGLTYPTLVWADYSDLYEREARKILETQVDAIEAADCVVARSHLLHGPPI
                     DAILDLCEELRPGLVIVGSRGLGPVRRMFVGSVSEGVVHHARCPVLVVRGGEEAWPPA
                     RIVAGDDGSKSAELAAELAGGIGAVCGAEGVLVTAYRTPPRPIGGWSAEDRRKLGEAR
                     SKEEENLNERAESLGVVMGRRSGTRLVDADATLALLQVAEEGEERKTLLAVGSRGLGA
                     VGRARLGSVSTKVLRAARGPILIHSSGP"
     gene            complement(6818..9061)
                     /locus_tag="VN178_00345"
     CDS             complement(6818..9061)
                     /locus_tag="VN178_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWS79439.1"
                     /translation="MTKSGTNSNRNIIFVGLMLGMLVAVTNQTMVSPAMPVMVAELGG
                     IEHYSWIATSALLAVAVSVPVVGKLSDIYGRRSFYIWGLIIFMLGSILAGMAQGFWWL
                     VGARAVQGLGMGVLQTLSQTIIGDIISPRERGKYMGYVGGVFGIALIAGPLAGGWITE
                     VFSWRWLFFVNLPLGVAALLFVIAFLHLPHVPMRHSVDYVGFVTLGLGLCAVLLATSW
                     GGAQYPWGSWQIISLYIASAVLLLGFIINEHYAEEPVVPLRLWKNSVFTLSNLSNVAV
                     GMAMFGAVFFIPLYVQGVIGASVTNSGALLIPLMFSAIVLSILVGRLITRTGRYKGFV
                     IAGLIVMGAGYYLLSRLGYDPTQTDLTVAMVLLGIGLGGVMQTYVLIVQNATSRRDLG
                     IATSITQLTRSMGATFGTAIFGTVMVGSMKSEIPKHLPPETLNSSQAERYFDGRGVGS
                     LLDPSALGQLSPAIATGIREGLSAALHSVFLVGLMIIGIALVASVFIKELPLRDTAFA
                     DEVIGEQVDPDPNAGEGAYARDRRDERFAGQQDTYLNVLGEEALLGLSMDRKAAGLEG
                     AMKGEVVRKLSAAVGSAVFFVLVPGTAVGLVPWWITDWEIREPLSYWAPARVVGASLI
                     VAGAAVLIHAFARFVVEGLGTPAPVAPPEHLVVGGFYRYVRNPMYVAVLAIIVGQALA
                     LGQLILLAYAVAAWIAFASFVHFYEEPALSRRFGARYEAYRRAVPAWWPRLRPWRTGE
                     RDSPGAR"
     gene            complement(9196..9645)
                     /locus_tag="VN178_00350"
     CDS             complement(9196..9645)
                     /locus_tag="VN178_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWS79440.1"
                     /translation="MDGMREKRLEEVAGEVNPIYASVGSAFKRMLFAFESEVGMSPPR
                     YFLLHMVANEGNISQGQVGQLSGVDPSRVTQVAKTLEGEGLIQRHRDPDDNRVVRMRL
                     TPEGRRVFEEASAKREALRARIRRVLSQEEENELRRLLDKVAEALEE"
     gene            9973..10611
                     /locus_tag="VN178_00355"
     CDS             9973..10611
                     /locus_tag="VN178_00355"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS79441.1"
                     /translation="MEAKELSPRGRAKRDQILGAARELFLERGFAGTSMEAIREEAGV
                     SKPTVYNYYPAKEELFVDVLRRHIDENFGDWLLEEAKGEDSPATREELRDVLRSLVER
                     FFATLMQPEYLALMRVVIAETPRFPYLGELLKQMAPERMMELVSTLLKRADERGIAEI
                     EDVDAARRMLIGPFITYVFLDGLLSGEPRPPEPERIESIIDLYMKVISGKSG"
     gene            10684..11856
                     /locus_tag="VN178_00360"
     CDS             10684..11856
                     /locus_tag="VN178_00360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWS79442.1"
                     /translation="MTQMREPVILEAVRTPFARAGGAFREVRPDALLAHTLDGLLERA
                     GLDGEKVEDVVNGTVTQAGEQGANIGRLGVLLAGFPVTVPAVSLNRMCGSSQQAAHFV
                     SQAVAAGDITYAIASGAESMTRAPMFSDTGNGETGFEVNPEILEKQDLVHQGESAERI
                     AQKWKISREDLDAFATESHRRASAVAREGKNKEILPTSGLDPDGNEMELTTDEGIRHK
                     IDAGKMASLKTVFRPEGEGVVTAGNSSQLTDGASALLVGDREVAEADGFTPRARFVAR
                     VAIGEDPVMQLTGVIPATRLVLERAGLSIDEMDWIEINEAFASVVLSWADELEPDMDK
                     VNPWGGAIAHGHPLGATGAGLMAKMLAGLQATDGRFGLQVMCIGHGMSTATILERI"
     gene            11857..11988
                     /locus_tag="VN178_00365"
     CDS             11857..11988
                     /locus_tag="VN178_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79443.1"
                     /translation="MDRQEMDRAIKRACRNTIGDAIRRSTKHPEDGDSGDENRGAER"
     gene            complement(12037..12393)
                     /gene="mnhG"
                     /locus_tag="VN178_00370"
     CDS             complement(12037..12393)
                     /gene="mnhG"
                     /locus_tag="VN178_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HWS79444.1"
                     /translation="MDSAFASAIPYVADALVILGVLVMTVGVYGAIRMPDTYTKLHAM
                     SKAVFLGVVSLCASSAVTGDPAIIYRAILIGVFLLVTTPISSFVIARAAFLRGERMET
                     ADACDESGRGLTRGKE"
     gene            complement(12393..12659)
                     /locus_tag="VN178_00375"
     CDS             complement(12393..12659)
                     /locus_tag="VN178_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HWS79445.1"
                     /translation="MHEIVFYLAFVWMTVLLVVSAAAVIRLRSTAARILALDMLTLIL
                     AALLVLYADATRSSYYMDAALILSLLAFMATLILGRYYGERKIF"
     gene            complement(12670..>12944)
                     /locus_tag="VN178_00380"
     CDS             complement(12670..>12944)
                     /locus_tag="VN178_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HWS79446.1"
                     /translation="VVGTWEVALVTLHLKPLERPGIVAVPVGDRTPFGVAIWTLATGL
                     PPGSYFVDVDPERRVALIHVLDASDPEAFREQQEVFYRRYQSKVFP"
BASE COUNT         2528 a         3982 c         4197 g         2237 t
ORIGIN      
        1 gctctctgcc gctgcgggag atcgatgacg aagccgttct gtgacggcac ccattcgaag
       61 gtcggtttcc gagcggcgga gagggccgtt cgactggaga gggggcgggt gtgatgatgt
      121 cggttacccg acgggtcgcc caagcggggc ttccgacccg ttacggcacc ttcgagatgt
      181 tcgtctacgg cacccccgat cataaggaac acgtggcgct caccctggga gcgatcgacg
      241 acggcggtcc ggttctggtc cgtgcacact cggaatgtct gacgggcgac gtgttcgggt
      301 cgtctcgttg cgactgcgga gaacaactgg gcgagagtct gcggttcttg caggagcagg
      361 gccgcggcgt cttgctctac ctgcggcagg aggggcgtgg tatcggcctt accagaaagg
      421 tctctgctta cgcgcttcag gagcagggct tcgacacggt cgaggcgaac cttgcattgg
      481 gattgcctga ggacatgcgc gactatcggg tcgcggccga gatgttgctc gacctcggcg
      541 tgcggcgggt ctgtctgctg accaacaacc cggcgaagat agaagggatc gagggttacg
      601 gcgtcgaggt agtagagcgc ataccgctgg ggatcacgcc gaacccctcg aatctcgagt
      661 atctgcgaac caagcgggag aagatgggcc acctcttgcc gatggccgag ccgctcgaag
      721 ttgcgtgtga aggatcgccc tggagaaagt ggcgtggatg aaagggagta tcgagtgcgt
      781 tatggcatcg tgatgtccaa cctggatgat tacgccgacc cgcgccaggc ggtcagcctg
      841 gctcgggcgg ccgaggaggc cggctgggag ggcttcttcg tgtgggatca cctcggcttc
      901 gtgtggggcg caccgtcgtg cgatccgtgg ggtgttctgt ccgcaatcgc cgtttccacc
      961 acccgtctca agctgggaac cgctattacc ccgctggcgc gccgtcgtcc gcatatcgtg
     1021 gccaataccc tggcgagcct ggatctcctc agtggtggac gcgtcatatt cggggctggg
     1081 cttgggggag ttgcgaagga gtttaccgcc ttcggcgagc cgggcgatgc gaaggtgcgt
     1141 gctcgtatgc tcgacgaggg gctcacggtt gtggatgggc tctggtcggg cgaaccgctt
     1201 acgcaccacg ggcaacacta cgacgtcgag aacgtgtccc tggcgcctct ccagcgtccg
     1261 cgcatcccga tctggatcgg cggagagggt gcgcccgccc ttcgacgcgc cgcccgttgg
     1321 gacggttggc tcgcgccggc cacgagtccc gacggcatgg ccaaagaccc ggagcgcatt
     1381 gccgaaatga tcgccgagat ccaacaccac cggagggcgg acacgccctt cgacgtggcc
     1441 gtggacggat actccgatcc cggagaccct gcgctgccgc gcgcttatga agcggccgga
     1501 gcgacctggt ggttggagag catccacggt atgcgcggct cggtggagga gacgatggcc
     1561 cgggtagaag cggggccacc agaagaagga ggagatgatg ctagcgatag ccgaagatga
     1621 gtttggaggg cccgtatccc tgatgacact tccagttcct gagatcgggg caggcgaggt
     1681 actgatccgc gtccgggccg ccggggtcaa ccccttcgac tggaaggtcg cagacggggt
     1741 tctcgaggac gagaaggaac atcgcttccc cctgatactc ggcttcgacg ccgcgggtgt
     1801 ggtcgagcgg gtcggttccg acgtgagagg gctctccgaa ggagacaaag tgtacgggta
     1861 cctctccaag ccggtgatgg ggcagggcgc ctacgcggag tacgtcggtg cccctgcgga
     1921 catcgttgcc aggaagcagg aagctgttag tttcgcagag gcggcggcgt tgcccatgcc
     1981 ggggcttacc gccatggatc tcgtagacgc ggtggacctc gaggagggcg agacagtctt
     2041 gatcgtaggg gccaccggcg gcgtcggctc ctacgcgacc cagctggccg ccggacgcgg
     2101 tgcccgagtg atcgctaccg cccgtcagga gaataaggtg ttcgcgcggg agctgggagc
     2161 agtgcagacg atcgaccaca ccagagagga tctcgcaaag tccgtgcgag ctgcccatcc
     2221 cgacggcatc gatgctgtta tcgatgtggt aagcgagggt gaagcgctcg accgtatagc
     2281 gaggctacta aaggagggcg ggcgcctcgc gtcgtcggtg tacgcggccg acatagaagg
     2341 gctagctcgc cgcagcatcc aggcgacgaa cgtaagcatg cagcccgacg cccggcggct
     2401 agaggagctc tcgcggatgg tcgacgccgg ggacctttcc gtgagactcg agcacgcctt
     2461 cccgctagag agagcacccg aggcgctgga agaaagcagg gccgggcacg tacgggggaa
     2521 gatcgtacta ctcgtcgact gaagggagcc aacacgcaat gcaaacaata gcctctccga
     2581 gagaaacgct cgcaacgccc gaacaattgg ctaggcgagc aggaagggtc ttgcgctaca
     2641 acgatatggg agattggacc cgtgccgctc ccaaccttta tcctcaccag tggagctggg
     2701 acagcgcatt catcgctatc ggtctcgccc acctcgacac ccgtcgcgct gccaaggagc
     2761 tgctcgccct cttttcccac cagtggagga ccggcaagat cccccacatc gtcttcaacc
     2821 aagaggctcc tccggagagc tacttcccgg gagcagagca ctgggcttgc gcctcgacct
     2881 ctcccgacgc cccgccagcc ccgccctaca ccagctgcct gtgccagcct ccggtccacg
     2941 ccatcgccgc cctgcgtatc tttgaggtag cccagagaaa caggggagag gatactcgag
     3001 ccgcccaggc tttcctgcag gaggcctacc ccagccttct gcgctggcac cgttacctct
     3061 cgacttacag ggatcccgag ggttccggcc tcgtaaccat ctaccatccg tgggagagtg
     3121 gcaccgataa ctctccccgc tgggacacgg tcctcgcggc tgtggaggtc ggcgacctgc
     3181 cgccttatcc ccggtatgac cttcagcacg tagccaaccg ttcccagagg cccaccgacg
     3241 cggactacga tcgctacctg tggctggtca agctcataaa gaaagccagc tgcgacgaga
     3301 ggagcatcta ccgctaccat ccgttcttgg tcaaagacgt gctcttcagc gcgatcctgg
     3361 tggcggctaa cgaggccttg ctagagatcg ccaggctggt cggggcgcaa gaagaagagc
     3421 gcgctttgat caccgattgg atcgagcgcg gacgtcgagg actcgcggcg aggtgggacc
     3481 ctgaagtggg cctgtgcctc gatcacgatc tgcgctccgg aagacccttg cgggtacgca
     3541 ccgtggccgg cttctcactc cttatagcgg gagggcagag tcccgaacag gtcgacgccc
     3601 taatggccac gcttgactca cccgcgttcc tcggacatcc ctggctacgc tggtcgttgc
     3661 cgccgagcac cagtcccctt caggagggat ttcaccggag cagttactgg cggggaccga
     3721 cctggccggt agtcaactgg ctcctgtggt ggtcgctgct ccgagccggc aaaccgatgt
     3781 gggccgagag gcttcggcaa gcgtctctac agcagatcgc gagcagcggt tttggcgagt
     3841 atttcgatcc atacaccggc gagcgtctcg gggcgaccga ccagtcgtgg acagcggccg
     3901 tcgccctgga ctggttggcg aatggacccc ggcgagtgga gagaaaggtg gcgtagaaag
     3961 tttgagggtc gaagcggtcc tgtacgctcc gatcagatcg cattgtcctt caagtccttc
     4021 agcggatatc gtgtgccccg ccactcgatg cctctgttaa tgatcgtggt ggatgcggac
     4081 ctgagcacag cgtagagaaa gacgcagata ccgaaggggt gaaggacagc gtaccaccac
     4141 ggcgtcttgt ctccaaagtg tctggcccgg taaacgtaga cgagaaaggt cgagaggatg
     4201 ttcatcctgc agagagttcc cgtcgttttg cgcgagaaga gaccgaagac agggagcacg
     4261 ttggtcagca agagcatcaa cacaccggcc accgtcgcgc cgatcctgta gtcaagcccg
     4321 gagaagatac ttttgctcag tccccgcacg gcatccggca gggcctggtg ccattccacg
     4381 ctgatgagtc ctaccccgta agccactccc tgcctgaaac catgcctctt gagaatcttg
     4441 gccagcttca tgtcgtcgtc gggacgcatg cggatggcgc ggtgggttcc gctcctctcg
     4501 taggcatctt ttctgatgag gttgaaggcc ccaaccccca cgtgctcctg tgcctgggga
     4561 tcgcccaccc gccagggccg ctgggtcatc tcgaaaacga gggtgaaggc ggcgacaaag
     4621 ctccggagca gaacgctcct gcacacgatc tcgggcggca gggtcaggtg atccaatccg
     4681 ttggtggtcg cgtagcggac cgcattgtcc gtgcagctgg gagtgaaaat tacatcggcg
     4741 tcggtgaaca ggagccattc tcctcgggcc tggatggcac cggcgtagag ggcgtaggtc
     4801 tttcccagcc agccatcagg caatgactcc acatccaaga tccgaagtcc gtccgggtgc
     4861 ttcgtcgcca gctcttccag gatctcgccg gtacgatcgg tggagcggtc gttgacggcg
     4921 attacctcta tcatgcccga ataatcttgg gccaacatgg agggtaagct ctcgcccaac
     4981 gaccgctcct cgtcgcgggc ggccaggatc acggagagtg ctgggttgcg gtcgacgaga
     5041 tcggtcccca gaacgtcttc gagcacgggt atcttcctta tgccaagcaa ccactgcagg
     5101 cataggccca gccataccag agagcctagg gccgcgagca acctcgacat gcttacaagc
     5161 tggtgtctgt ggcccacaaa ggtcctcacg aggcacactg ttgcacacct gctgcgagac
     5221 taccagagcc agagtgccca agatcgggtc ataacaagct taccttttcg gactatcgct
     5281 acggcgcccg gcgactcgcg cacctggcac tcgtatactg gtgtggtagg agcccaggcg
     5341 aggagatcca gggatgggac ccgccccgat ccacaaggcg caacttctgc acgaaccact
     5401 cccgcccaga tgagaccggg aacgcggcac gcaggaaagg agcgcgcttc gatggtcacg
     5461 ccgcacgaca acatcataag accaaacagg ctgctgctgg cgaccgatgg ttctgaagac
     5521 gcagtgctcg ccgcaagggt cgctaccgac ctctcccgta gaacgggtgc caggctgcac
     5581 gtggtccacg cctggtatca cctcgtaaag ggccttacct acccgacgct cgtctgggcc
     5641 gactactccg atctgtacga gcgggaggcc aggaagatcc tcgagaccca ggtggatgcg
     5701 atcgaggccg cggattgcgt agtcgcccgg tcacatctcc ttcatggccc accgatagac
     5761 gccatcctcg acctctgcga ggagcttcgg cccggtcttg tgatagtggg cagccgcggc
     5821 ctcggaccgg tcagacgcat gttcgtcggt agcgtctcgg agggtgtcgt ccaccacgcg
     5881 aggtgtcccg tgctcgtcgt acgtgggggc gaggaggcct ggcctcccgc aaggatcgtc
     5941 gctggtgatg acggctcgaa gagcgccgaa cttgcggcgg agcttgcggg gggcataggc
     6001 gccgtctgtg gggccgaagg cgtgctggta acggcctacc ggacccctcc gcgtccgata
     6061 ggaggctgga gcgccgagga tcgtcgcaag ctcggagaag cccgctcgaa ggaagaagag
     6121 aacctgaacg agcgggccga gtcgctcgga gtggtcatgg gcagacgctc ggggacgcgg
     6181 ttggtcgatg cggacgctac gctagccctg ctgcaggtcg ccgaggaggg tgaggagcgc
     6241 aagactctgc tcgccgtggg gagccgcggg ctcggcgcgg tgggccgtgc gcggctaggc
     6301 agcgtttcga ccaaagtgct cagagcagcg agaggcccga tcctgatcca ctcgagtggg
     6361 ccataagatg cgcagcggca cgacgatgta tcccgctccg gtaatctcga cctccggctc
     6421 gggctgctcg caggggcagg ttcgcaacaa ccagtgcgag gcgtcttcgt acaccttcag
     6481 ctcgccgcgg gggtgcggag agcatagatt ctgccggccc tcgctccgcg cgggcgttgc
     6541 gcggttactg gcgaggtttc cttcgacctg caagaagcgc tgggagaaag ttgttggctt
     6601 acggcactcg tccgccaagg tagccaacgg gacacgctgc tcggaagcca ggtggtgatg
     6661 cctgggcagc gtcgccgtct cctcacgtcc tggtgcggta gtggaggtag accatgccgt
     6721 tgccaaaacg gcgttcgtcc agcagttcga gcctcacacg gacatcatcg gagagggctt
     6781 gtttgcctcc tcccaccacg atgggtgtga ggactcgtca tcgtgcgccc gggctgtcac
     6841 gctcgccggt tctccacgga cgcaggcggg gccaccaggc tggcacggca cgccggtatg
     6901 cctcgtaccg ggcgccgaag cggcggctta gcgccggctc ctcgtagaag tggacgaacg
     6961 aggcgaaggc gatccaggcg gccacggcgt aggccagcag tatcagctgg ccgagggcca
     7021 aggcttgacc gacgatgatc gcgagaactg ccacgtacat cggattgcgg acgtaccggt
     7081 agaagccgcc gacgaccaga tgctcgggcg gcgcgacggg cgcaggagtt cctaggcctt
     7141 ccacgacgaa tcgtgcgaac gcgtggatca gcacggccgc gcccgcgacg atgagggaag
     7201 cgcccaccac ccgcgctggc gcccagtagg ataaaggctc gcgtatttcc caatccgtta
     7261 tccaccaggg aaccaggccg acagcggtgc ctggtaccag cacaaagaac acggcgctac
     7321 cgacagcagc agatagttta cgcacgacct ctcctttcat ggcaccctct agcccggctg
     7381 cttttcggtc catagagagt cccagcagcg cttcctcgcc gaggacgttg aggtaggtgt
     7441 cctgctggcc ggcgaacctt tcgtctctac ggtcgcgcgc atacgctccc tctccggcgt
     7501 tcgggtctgg atcgacctgt tctccgatga cttcgtcggc gaacgccgta tcacgcagcg
     7561 gcagctcctt gataaagacg ctcgccacca gggctatgcc gatgatcatg agtcccacga
     7621 ggaacaccga atgcaacgcc gcggaaagcc cttcacggat gccggttgcg atggccgggg
     7681 atagttgacc gagggcgctc gggtcgagca acgaccctac ccccctacca tcgaagtacc
     7741 gttcagcctg cgaactgttc agggtctcgg ggggcaagtg tttggggatc tcggacttca
     7801 tgctaccgac catgacggtg ccgaagatag cggtaccgaa cgtggcgccc atcgaacggg
     7861 tgagctgcgt gatcgaggtg gcgatgccga ggtcccggcg cgaggtcgcg ttctggacga
     7921 tgaggacgta ggtctgcatc acccctccga gaccgattcc gaggagtacc atggccaccg
     7981 tgaggtcggt ctgcgttggg tcgtatccga gacgactcaa cagatagtac cccgcaccca
     8041 tgacgatgag cccggcgatc acgaacccct tgtagcgacc ggtgcgggtg atgagcctcc
     8101 ccacgagaat gctgagcact atcgcggaga acatcagcgg gatgagtagg gcccccgagt
     8161 tcgttacgct cgccccgatg acgccctgca cgtagagggg gataaagaag actgcgccga
     8221 acatggccat gccaacggcc acgttagaaa gattcgacag ggtgaagacg gagttcttcc
     8281 agagcctcaa gggcacaacc ggctcctcgg cgtaatgctc gttgatgatg aatccgagaa
     8341 ggagaaccgc actcgcaatg tatagggaga tgatctgcca cgagccccac ggatactgcg
     8401 caccacccca tgaggtcgcg agcagcacgg cacagagccc aagccctaga gttacgaaac
     8461 ccacgtaatc tacggagtgc ctcataggga catgaggtag gtgaaggaac gcgattacaa
     8521 acaagagcgc cgcgacgcca agcggcaggt tgacgaagaa gagccatctc caggagaaga
     8581 cctcggtgat ccaaccacct gccaaagggc cggcgatcaa ggcgataccg aagacgccgc
     8641 ccacatatcc catgtatttg ccgcgctcgc gggggcttat gatgtcgccg attatggtct
     8701 gggaaagcgt ctggaggacg cccatcccca aaccctgtac ggctcgtgcg ccgaccagcc
     8761 accagaaccc ctgagccata ccagccagga tcgagccgag catgaagatg ataagccccc
     8821 aaatgtagaa ggagcgccgg ccgtagatgt cggagagctt gccgaccacg ggcacgctca
     8881 ccgccacggc cagaagcgcc gaggtcgcga tccagctgta gtgctcgatc ccgccgagct
     8941 cggccaccat gaccggcatt gcaggcgaga ccatagtctg gttggtaacc gcgacgagca
     9001 tcccgagcat gagcccgacg aagatgatgt ttcgattgct gttggtgccg ctcttggtca
     9061 cgaaccttcc gtcagtcctc catcgcttct ggctgcttcc ccaacaagcg ccgcagctcc
     9121 tgatgttctt cctcatcgtg gaggggaagc accggtttcg cggtcgtgtc ttgcctcgtg
     9181 ttaatggatg ctcctctatt cctccaaggc ctcggcaacc ttgtccaaga gcctcctgag
     9241 ctcgttctcc tcttcctggc tcaggacgcg tcggatccgg gccctcaagg cctcgcgctt
     9301 ggcggaagcc tcctcgaaaa ccctccggcc ctccggcgtc aggcgcatac gcacgacccg
     9361 attgtcgtcc gggtcgcggt gcctctggat caacccttcc ccctccaagg tcttggccac
     9421 ctgcgtcacg cgcgaagggt ccacaccgga gagctggccc acctgcccct ggctgatgtt
     9481 cccctcgttg gccaccatgt ggagcaggaa atacctcggg gggctcatcc cgacctcgct
     9541 ctcgaaggca aagagcatcc tcttgaaggc cgacccgacc gaagcgtaga tcgggttgac
     9601 ctccccggca acctcctcga gacgcttctc acgcatcccg tccatgccca ctcctctcct
     9661 gcaaaccctt cgatgaccag gatatcagag aatactttat ccatcaacta tttatccatc
     9721 aaagatcatt gggacggacc atccacgatc ctcgttccgc atgggctcga actcgaaggt
     9781 ctggtggtag accgcggcgg catgatgggc tactcgtgat agatcctgcc tcctacgccc
     9841 cgccaaactc aggacgttcc ttctctcttg acccacacct gtttctacct tgacaacctg
     9901 cctcgaacag gttacactac acggtacagt ttataccaca cagtttagtg taatgaaagg
     9961 aggtgctcgg atttggaggc taaagagtta tcaccgaggg ggcgcgccaa gcgggaccag
    10021 attctcgggg cggccaggga gttgtttttg gaacgagggt ttgccgggac cagcatggag
    10081 gccatcaggg aggaggccgg ggtctccaag ccgactgtct acaactatta tccggccaag
    10141 gaggagttgt tcgtggacgt cctgcggcgg cacatcgacg agaacttcgg cgactggctg
    10201 ttggaggagg ctaagggcga ggattctccc gccacccgcg aggagttgcg ggacgtactg
    10261 aggagcctgg tggagaggtt cttcgccacg ctgatgcagc ccgagtacct ggcgttgatg
    10321 agggtggtta tcgccgagac gccccgcttt ccctatctcg gggagctcct gaagcagatg
    10381 gcaccggagc ggatgatgga gctcgtctcc acactgctga agcgggctga cgagcggggg
    10441 attgccgaga tcgaggatgt ggatgccgcc agacgtatgc tcatcggccc tttcatcacc
    10501 tacgtgttcc tggatggtct gctttcgggc gagccgcgtc ctcctgagcc agagcgcatc
    10561 gagagcatca tcgacctgta catgaaggtc atctccggga agagcggatg aagagaacga
    10621 agagaacgag accgcgttcg tgtgggaggg aaggcaacct cctacgagag gagtagacaa
    10681 atcatgacac agatgagaga gcccgttatc ctcgaggcgg tgcgcacccc gttcgccagg
    10741 gccggcggcg ctttccgcga ggtacgtccc gacgcccttc tggcgcatac cctcgacggg
    10801 ctcctggagc gcgcagggct agacggagag aaggtcgagg acgtcgtgaa cgggaccgtt
    10861 acccaggccg gcgaacaggg ggcgaatatc ggccgtctcg gcgtgttgct cgcgggcttc
    10921 ccggtaacgg tgcctgcggt aagcctcaac cgcatgtgcg gctccagcca gcaggcggcc
    10981 cacttcgttt cgcaggccgt agccgcgggg gacataacct acgccatcgc ctcgggcgcg
    11041 gagagcatga ctcgggcgcc gatgttcagc gacaccggta acggcgagac gggcttcgag
    11101 gtgaacccgg aaatcctgga gaagcaggac ctcgttcacc agggcgagag cgccgaacgc
    11161 atagcccaga agtggaagat atcgcgcgag gacctggacg ccttcgccac cgagagccat
    11221 cggcgcgcga gcgcggtggc ccgtgagggc aagaacaagg agatcctacc cacttccggt
    11281 ctcgaccccg atggtaacga gatggagctc acgacggacg agggcattcg ccacaaaata
    11341 gatgccggga agatggcgag tttgaagacc gtcttcaggc ctgagggtga gggggtcgta
    11401 acggcgggca actcctccca gctcaccgac ggggcctctg ccttgctcgt cggggaccgc
    11461 gaggtggcgg aggcagacgg cttcaccccg cgggcgcgct tcgtggcccg cgtcgccatc
    11521 ggtgaggatc cggtgatgca gctgaccggc gtcatcccgg ccacacgcct ggtgctcgaa
    11581 agggccggct tgagcatcga cgagatggac tggatcgaga tcaacgaagc cttcgcctcg
    11641 gtcgttctct cctgggccga cgagctcgaa cccgacatgg ataaggtaaa cccgtggggc
    11701 ggggccatag cccacggcca tccactgggc gccaccggcg ccggcctgat ggcgaagatg
    11761 ctcgccggcc tccaagccac cgacggccgg ttcggcctcc aggtcatgtg catcggccac
    11821 ggcatgtcga ccgcaacgat tttggagcgc atctagatgg ataggcaaga gatggatcgc
    11881 gcgataaaac gcgcttgccg caacaccatc ggcgatgcca tcaggcgctc gaccaagcac
    11941 cctgaggacg gcgactcggg tgacgaaaac cgcggagcgg aacggtgaac gcgatacgga
    12001 ccggcgagaa ggaggcgccg gaggagaccg gccagctcac tcctttcccc tcgtgagccc
    12061 gcgtccggac tcgtcgcacg cgtccgccgt ctccatcctc tccccgcgca ggaacgcggc
    12121 gcgcgcgatc acgaaggacg agatgggggt ggtgacgagc aggaacactc ctatgaggat
    12181 cgccctgtag atgatcgccg ggtcgcctgt cacggccgat gaggcgcaca gcgagaccac
    12241 gccgaggaat accgccttgc tcatcgcgtg cagcttggtg taggtgtcgg gcatccggat
    12301 cgcgccgtag actccgacgg tcatcaccaa gacacccagg atgaccaggg cgtcggccac
    12361 ataggggatg gcggatgcga acgcagagtc cactagaaga tcttcctttc cccgtagtag
    12421 cggcccagaa tgagggtggc cataaatgcc agcagggaga ggatgagcgc cgcgtccatg
    12481 tagtacgagg agcgggtggc gtcggcgtag aggacgagca gggcagccag gatcagggtc
    12541 agcatgtcga gcgccaggat gcgcgcggcc gtcgagcgca gacgaatcac cgccgcagcg
    12601 ctcactacga gcagtaccgt catccacacg aaggccaggt agaagactat ctcgtgcatc
    12661 gggacctcct caagggaata ctttgctctg gtaccggcgg tagaagacct cctgctgctc
    12721 gcggaaggct tcggggtcgc tagcgtcaag tacgtggatc agggcgactc tccgctccgg
    12781 gtccacgtcc acgaagtagg atcccggcgg gagtcccgtg gccagcgtcc agatcgccac
    12841 cccgaacggc gtccggtcgc ccaccggcac ggccacgatg ccgggcctct cgaggggctt
    12901 caggtgaagc gttaccagcg cgacctccca ggtgcccacc acta
//
LOCUS       DATMVF010000011         6939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_461475, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000011 DATMVF010000000
VERSION     DATMVF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461475"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1213)
                     /locus_tag="VN178_00385"
     CDS             complement(152..1213)
                     /locus_tag="VN178_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWS79447.1"
                     /translation="MSATSGTPIELEHASRNYGSGNERVRAVDDVSFTIEPGQTLCLV
                     GESGCGKSTTGRMAAGLLEPSQGRVLYGGKAVEALPEEEKKRFRRAVQIIHQDPYASL
                     NPIRNVTQILSAPLQRHRLVKSRSELKSRLHELLETVGLTPPEEFLNKHPHQLSGGQR
                     QRVSVARALTVGPEFIVADEPVSMVDVSLRIGLLNMLLGLQKDLGVAFLFITHDLAVA
                     KHFSWEGRIGVMYLGRMVELGPTQRLVGEPVHPYTRALISALPEADPELTRSKERLRL
                     RSPDIPSLLNLPAGCPFHPRCPMYEEGLCDTDRPAEDAVDLEGHLAACYVAARELGQE
                     DRIPESSRAAGAEAERRTA"
     gene            complement(1179..2237)
                     /locus_tag="VN178_00390"
     CDS             complement(1179..2237)
                     /locus_tag="VN178_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS79448.1"
                     /translation="MEGRDEVLSVRDLEIGYATGRGLVRAVRGVSFDLGDDESLAFIG
                     ESGSGKTTLGLSLVRLLPQNASIARGEIHFRTASEEALDLTTLDDEALRRFRWRECAM
                     VFQSALNALNPVMRIRDHFADTAKDHGETNGSLKGRALTERATECLQMVQLDPDRVLG
                     SFPHELSGGMRQRVMIALGLLLNPRVLILDEPTTALDVLTQRAIIEVLRTLKDEHRFA
                     MIFVSHDLSLAAELADRVATTYAGRIVELGEVERIFYHPRHPYTVGLLDAVPTLAGEQ
                     QELSSIPGSPPDLIDLPAGCKFHPRCPYATERCREEDPPLMEVAPGQAAACWHWEKVG
                     SDWEARGGERHERDAHRA"
     gene            complement(2242..3150)
                     /locus_tag="VN178_00395"
     CDS             complement(2242..3150)
                     /locus_tag="VN178_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79449.1"
                     /translation="MAATTGESRFRDSAVGRVLGWFASTLKLMARNKVGFAGFLVVST
                     IVLVTFIGPIFLPSEVPVDVKEIYETPSLSHPLGTDSEGKDVLLQIINGGRPILTIGF
                     LAAAISTTIAVTLGSLSGFLGGRADTGFTWLADMVLTIPQLPLLAVLAGILRLNSKIA
                     VAGLIGVLFWPTLMRAIRAQVLSLKERDYVESARALGLGTPHIVFREIAPNMAGYIAI
                     NFTLAVTASMYFVVALTLLGLVPLSEDNWGVMLNLAWTRGAIFSKDSIFYIMAPIAAI
                     ALFQLGLVAMNRSLSEIFDPRLRRGS"
     gene            complement(3155..4177)
                     /locus_tag="VN178_00400"
     CDS             complement(3155..4177)
                     /locus_tag="VN178_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79450.1"
                     /translation="MRKIASSYLLRRIAKALITIFVMASLTFFLVRLMPGGPVEAYIA
                     NLVTQYNLSHENAEAQAASLFSLDLNAPVYQQYLEYLGNLARGDLGESLVSRGVPVSE
                     IVMAYLPWTLFSIGTALIISFSLGIMLGMLMAYKRETKLDHILTNLGSIVSSIPDYLM
                     GILILVWVGVQWELIDVTNMRGSLSPGVQPGFNLTFIADAFYHASLPILTYVLGTIGL
                     WMLTMRGSTESTLGEDYVTVAKARGLKERRIATAYVGRNAALPPFTQLSISIALVFGG
                     SLLIEWIFVYEGLGYLLYNAIQQRDYPVMQGVFLIITTMVIVANLLADLLYSRLDPRI
                     RIAGRD"
     gene            complement(4314..6185)
                     /locus_tag="VN178_00405"
     CDS             complement(4314..6185)
                     /locus_tag="VN178_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HWS79451.1"
                     /translation="MASIEDLILLPKPRSVVARSGAYRLTGGSKIICQGDPGSLFPVA
                     RRLQRALWEDQRIGWALRAGDPGNGGSRGETTITLTPVQGIPAQGYRLRIADDGVDLI
                     AGDAAGAFYGVMTLVQMLRQCEGALPAGEIEDSPDFPSRGVMLDISRSKVPTMETLFG
                     LVDMFSGWKINHLELYTEHTFAYENHREVWAQASPMTGEEILRLDAYCRERFIELVPN
                     QNSFGHMHHWLELPRYNHLAECPEGFELTWGEHTRQMPPFSLNPTDRRSVEFMGELFS
                     ELLPHFSSEKFNVGCDETWDVGRGRSARAVEEKGAGRVYLDYVLGIYDLVKRHDRTMH
                     FWGDIIIQHPDLVPELPNDAVVLEWGYEADHPFDEHGGEFARSGMPFFVCPGTSSWNT
                     IAGRTSNCLGNVRNATENGLRHGASGVLNTDWGDNDHTQYLPVSYLGFAAGAALPWCH
                     ETNRDEDFISALDLHAFYDTARVMGRMSHDLGKAHELAGPAPHNSTVLFHLLTQSPDD
                     PLPEGVTVDSLREAGERIASIMEPLEGARMDRDDAEVTRDEFANAARMMLHACQRGVA
                     MLEGTIDSAEKRDELASQMRTILGEHRRLWSARNRVGGLQDSEGVFEQRLREYAGAV"
     gene            6466..6657
                     /locus_tag="VN178_00410"
     CDS             6466..6657
                     /locus_tag="VN178_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79452.1"
                     /translation="MDHEEVGRYWDENAEVWTELVRAGYDHYRDGLNTPAFLEMLPDV
                     TGLSGLDVGCGEGYNSWGP"
BASE COUNT         1306 a         2205 c         2165 g         1263 t
ORIGIN      
        1 gttacagcca cgagaaagac cactcccata accagggtca agagggtgat tgcatactcg
       61 ggggtcccgg ggtccagaaa gaacaagagt aagaaagacg gcacgactaa aaggaaccct
      121 atccggaatc cggccttccc cagttccggg atcacgccgt cctccgttcg gcctcggcgc
      181 ccgccgccct gctgctctcg ggtatgcgat cctcctggcc gagctccctg gccgcgacgt
      241 agcaggccgc cagatgtcct tccagatcga cggcatcctc ggcgggacgg tccgtgtcgc
      301 acagaccctc ctcatacatc gggcagcgag gatggaaagg acaacccgcg ggcaggttca
      361 acaggctggg gatgtcaggg ctgcgcaacc gcaaccgctc tttgcttcgc gtcagttcgg
      421 gatctgcctc cggcagcgcc gagatcaggg cccgcgtata aggatgcacg ggctccccga
      481 caagccgctg cgtgggaccg agttcgacca tgcgcccgag gtacattacg ccgatccgcc
      541 cttcccagga gaagtgcttg gcgacggcga ggtcatgggt gatgaacagg aacgccaccc
      601 cgaggtcctt ctgcaacccc agcaacatgt tgagcagccc gatgcgcagc gagacgtcga
      661 ccatcgagac gggctcgtcg gccacgatga actcggggcc gacggtgagc gccctggcga
      721 cggagacgcg ctgccgctgc ccgcccgaga gctggtgggg atgcttgttc aggaactcct
      781 cgggtggcgt cagccccacc gtctcgagca actcgtggag cctgctcttt agctcggagc
      841 gcgacttcac gagcctgtgg cgttgcaagg gcgcgctcag gatctgcgtt acgttgcgta
      901 tcgggttcaa cgaagcgtaa ggatcctggt ggatgatttg gaccgcccgc cggaagcgtt
      961 ttttctcctc ctcggggagc gcttcgaccg ccttgccgcc gtagagcacg cggccctgcg
     1021 aaggctcgag cagtcccgca gccattcgtc ctgtggtgga cttgccgcac ccgctctccc
     1081 ccacaaggca cagggtctgg cccggctcaa tggtgaaaga tacgtcgtcg acggccctca
     1141 cccgctcatt gcccgaaccg taattgcggc tggcgtgctc aagctctatg ggcgtcccgc
     1201 tcgtggcgct caccccccct cgcttcccag tccgaaccta ccttctccca gtgccagcat
     1261 gctgccgcct gcccgggagc cacctccatc agtggagggt cctcctcgcg gcacctttcc
     1321 gttgcgtagg ggcatcgcgg gtggaacttg cagcccgcag ggaggtctat aaggtctggt
     1381 ggggagccag ggatcgagct cagctcctgc tgctctccgg ccagggtggg caccgcatcg
     1441 agcaggccga ccgtgtaagg gtggcgcggg tggtagaaga tccgctctac ctccccgagc
     1501 tcgacgatac ggcccgcgta ggtcgtggcc accctgtcgg ccagctcggc ggccagggaa
     1561 aggtcgtggg agacgaagat catggcgaag cggtgctcgt ccttgagggt cctcagcacc
     1621 tcgattatgg ccctctgggt cagcacgtcc agcgcggtgg tcggctcgtc cagtataagg
     1681 acccttgggt tgagcaggag accaagcgct atcatgaccc gctggcgcat accgccggag
     1741 agttcgtgcg ggaaggagcc gagcacccgg tccgggtcca gttgcaccat ctgaaggcac
     1801 tccgtggccc gctccgtgag ggcgcggcct ttgagggaac cgttcgtctc gccgtggtcc
     1861 tttgcggtat cggcaaaatg atctctgatg cgcatcacgg ggttgagcgc gttgagggcc
     1921 gactggaaca ccatcgcgca ctccctccac cggaagcgcc tgagcgcctc gtcgtccagg
     1981 gtcgtaagat ccaaagcctc ctcagaagcg gtgcggaagt ggatctcgcc cctggcgatc
     2041 gaggcattct gcggcagcag acgcaccagg gagagaccga gcgtagtctt gccggacccg
     2101 ctctccccaa taaatgccag gctctcgtca tccccgaggt cgaagctaac cccgcgcacc
     2161 gccctcacca ggccccggcc cgtcgcgtag cctatctcca ggtcacgcac gctaaggact
     2221 tcgtcgcggc cttccaccct ctcaagaccc ccgccgcagc cgcgggtcga agatctccga
     2281 gagcgagcgg ttcatggcca cgaggccgag ctggaacagg gctatggcgg cgatcggagc
     2341 catgatgtag aagatgctgt ccttggagaa gatcgccccc cgcgtccagg ccaggttgag
     2401 cattacgccc cagttgtcct cagagagcgg cacgagtcct aggagcgtca gggcgacaac
     2461 gaaatacatg ctagcggtaa cggccagggt gaagttgatg gcgatgtagc ccgccatgtt
     2521 gggcgcgatc tcgcgaaaaa cgatgtgcgg cgtccccaga cccagcgccc tggccgactc
     2581 gacgtagtcg cgctccttca acgagagcac ctgggccctt atggcacgca tcaatgtcgg
     2641 ccagaacaga accccgatca ggcccgcaac tgcgattttg ctgtttagcc tgagaatgcc
     2701 ggcaagcacg gccagaagcg gtagctgggg gatggtaagc accatatcgg ccagccaggt
     2761 aaagcctgtg tcggcacgtc cgcccaggaa accgctcagc gatccgaggg tcacggcgat
     2821 ggtcgtggag atggccgcgg ccagaaatcc tatcgtcagg ataggtctgc ctccgttgat
     2881 gatctgaagc agcacatctt tgccttcgga gtcggtgccc agaggatggc ttagagaggg
     2941 tgtctcatag atctccttta catccaccgg aacttccgaa ggcagaaaga tgggcccgat
     3001 aaacgtaacg agtactatgg tcgagacgac cagaaaaccc gcgaaaccaa ctttgttgcg
     3061 ggccatgagc ttgagcgtcg aggcgaacca tcccaggacc ctgccgaccg cagagtccct
     3121 gaagcggctc tcgcccgtcg tggcggccat cctgtcagtc cctacctgcg atccggatgc
     3181 gcggatcgag cctggagtag agtaggtcgg cgagcaaatt ggccacgatg accatcgtcg
     3241 tgatgatcag aaagacccct tgcatcacag ggtagtcgcg ctgctggata gcgttgtaga
     3301 gcaggtagcc gaggccttcg tagacgaata tccactctat gaggagtgat ccgccgaaga
     3361 cgagcgctat ggagatgctc aactgggtaa agggcgggag cgccgcgttg cggcccacgt
     3421 aggccgtggc gatcctgcgc tccttcaggc cccgcgcttt ggccacggtc acatagtctt
     3481 cgcccagtgt cgactcggtg ctgcctctca tggtgagcat ccagaggccg atagtgccga
     3541 gcacgtaggt gagtatgggc aaggaagcgt ggtagaacgc gtcggcgata aatgtgaggt
     3601 tgaagccggg ttggacccct ggtgagagcg agccccgcat gttcgtaacg tcgatcaact
     3661 cccactggac tccgacccac accaggatga ggattcccat aaggtagtcg gggatggagc
     3721 tgacgatgga ccccaggttc gtaagtatgt ggtcgagctt cgtctcccgt ttgtaggcca
     3781 tgagcatccc gagcatgata cccagagaga aactgattat gagtgccgtg ccgatgctga
     3841 agagcgtcca tggcaggtaa gccatgacga tctcactcac gggtacgccg cgcgagacga
     3901 gagactcacc gaggtctcct cgcgccaggt tgcccagata ctccaggtac tgctggtaga
     3961 caggcgcgtt caggtcgagc gagaagagcg aggcggcttg cgcctccgcg ttttcgtgcg
     4021 acaggttgta ctgggtgacc aggttggcga tgtacgcctc cacgggaccg ccaggcatga
     4081 gccggacgag gaagaaagtc agggaagcca tgacgaagat ggttatgagc gccttggcta
     4141 tccggcggag caggtagctc gaagcgatct ttctcaacgc gcctaacgcc ccgccccctt
     4201 gagccggtgc agtcgcccga gccaatctct taatcccttc cacctgccaa tgtggccctt
     4261 atacatatag acgaaagtac ctctcagcgc aagcgtgccg ccacgaaccc atgctacacg
     4321 gctcccgcgt actcccgaag ccgctgctcg aagacgccct cgctgtcttg caggccgccg
     4381 acgcggttgc gggcgctcca taagcggcgg tgctcgccca gaatggtacg catctgggaa
     4441 gccagctcat cgcgcttttc agctgagtcg attgtccctt ctagcatcgc caccccccgc
     4501 tggcaggcgt gcagcatcat ccgcgcggcg ttggcgaact cgtcgcgggt gacctccgcg
     4561 tcgtccctgt ccatgcgcgc accttcgagc ggctccatga tagaggctat gcgctcgccg
     4621 gcctccctga ggctatcgac ggttacaccc tcggggagag gatcgtcggg ggactgcgtc
     4681 agcaggtgga agaggaccgt ggaattgtgc ggggccggcc ccgccagctc gtgcgctttg
     4741 cccaggtcgt ggctcatcct acccatgacg cgcgctgtgt cgtagaaagc atggaggtcg
     4801 agggccgaga taaagtcctc gtccctgttc gtctcgtggc accagggcag tgccgccccg
     4861 gctgcgaacc cgaggtagct caccggcagg tactgcgtgt ggtcgttgtc gccccagtcg
     4921 gtgttgagca ccccgctggc tccgtgcctg aggccgttct cggtcgcatt gcggacgttg
     4981 ccgaggcagt tggaggtgcg ccccgcgatg gtgttccacg atgaggtgcc gggacacacg
     5041 aagaacggca tccccgacct cgcgaactcg ccgccgtgct cgtcgaaggg atggtcggcc
     5101 tcgtagcccc attccagcac cacggcgtca ttcggcaact cggggaccag atcaggatgc
     5161 tggataatga tgtctcccca gaagtgcatc gtgcggtcgt gacgctttac cagatcgtag
     5221 atgccaagca cgtagtccag atatacgcgg ccggcgccct tctcctcgac tgccctggcg
     5281 ctcctgccac ggccaacgtc ccaggtctcg tcgcagccaa cgttgaactt ctcactggaa
     5341 aagtgcggca gcagctcgga gaacagctct cccataaact cgacgctgcg tctgtccgtc
     5401 gggttcaggc tgaagggcgg catctgcctg gtgtgttctc cccacgtcag ctcgaagcct
     5461 tcgggacact ctgccaggtg gttgtagcgg ggcaattcca gccagtgatg catgtgcccg
     5521 aaggagttct ggttcggtac cagctcgatg aacctctcgc gacaataggc gtcgagccgg
     5581 agtatctcct cgcccgtcat cgggctggcc tgcgcccaga cctcgcggtg gttctcgtag
     5641 gcgaaggtgt gctccgtgta cagctcgagg tggttgatct tccacccgga gaacatgtcg
     5701 accagaccaa acagcgtctc catggtgggg accttgctcc tgctgatgtc gagcatcacc
     5761 ccgcgcgatg gaaaatccgg cgaatcctcg atctcacccg cagggagcgc cccctcgcac
     5821 tggcgcaaca tctgcaccag ggtcatgacg ccgtagaacg cccccgccgc atcaccggca
     5881 atgaggtcta cgccgtcatc agcgatccgt aaccggtagc cttgcgccgg gatgccctgc
     5941 actggcgtga gcgttattgt agtctcgccc ctggaaccgc cgttcccggg atctccagcc
     6001 cgcaaggccc atccgatacg ttggtcctcc caaagcgccc gctgcaaccg gcgggccacg
     6061 gggaacagtg atccaggatc cccctgacag atgatcttcg agccgccggt gagcctgtaa
     6121 gcgccggacc tggcgacgac cgagcgcggc ttcgggagca ggatcaggtc ttcgatagat
     6181 gccatagctt gggtgagtct cccctctctg tatttccttt gtcccgtcga atgtcgggaa
     6241 cacacttacg ggtgcctatc cccgtgccag cactggcctc cttccgtccc caccaacgga
     6301 ccactccttc ccaccacggg catgatgcca cgtggtagag ttcggcgcat cctacgcaac
     6361 acagcctggg gtaccttttc gacctatctc cattaccaga gacgaggata cggtcagctg
     6421 ggaagcgtcg atgatatcca cccgaccatc ggaggggaaa ggcgcatgga ccatgaggaa
     6481 gtaggccgct actgggacga gaacgccgag gtgtggaccg aactggtgcg ggccgggtac
     6541 gaccattacc gcgacggcct gaacaccccg gccttcttgg agatgctccc cgacgtgacc
     6601 ggtctctccg gcctggacgt cggctgcggg gagggataca actcatgggg accttgagtt
     6661 cctggctgaa caggttggtc gaggcggatt tcgtactgga gcgcttcggt gaaccgtatc
     6721 ccagcgacga ggccgtacaa gagcgtcccg gcctgtaaga cgcccagatc gttgcgtatt
     6781 tcctacacat cagggcgaga aagccggccg caacgaggag ctaaccggag cccgcgccgt
     6841 ccaatgcggg tagcgcggtc cgcgcgtagc gggcagcgta agccgtgcct gcgtcgttgg
     6901 agaactcgcc gggctctctg ctggtaaact cccccgccc
//
LOCUS       DATMVF010000012         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_276973, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000012 DATMVF010000000
VERSION     DATMVF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276973"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..961
                     /locus_tag="VN178_00415"
     CDS             119..961
                     /locus_tag="VN178_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79453.1"
                     /translation="MEGNDDRRLRPDEVEMILRRASELNSRRGMRGSYETPSVSPEVL
                     VQVAAAAGIPEQDVRRAMWDIASERTAEPRSFARKFYGPARLRAVREVEHPAERTREY
                     LEGLLRLQQGLKLRRKTEVGSLWDPGDELGVVRRTLDFSGDRTLLKAKSVELRVEGLG
                     EGRCEANLTADLSNQRGEYISLAGILGATLAALFMLAGMQNPLFFLGFLPMLVAPAFG
                     FRFAYSRASLELRRALDELLDASEESQPQEEPQDRNDRPRGQIQGLEPIPRFSPNRSK
                     RVEP"
     gene            1061..3217
                     /locus_tag="VN178_00420"
     CDS             1061..3217
                     /locus_tag="VN178_00420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HWS79454.1"
                     /translation="MPHRVLKEIFGFDSFRPGQEEVIRAVLEGRDTLAVMSTGGGKSL
                     CYQVPALMQESLTVIVSPLISLMKDQVDSLLQSSVFDAATLHSGLSPEERWEVERRVR
                     TGEVKMLYVAPERLRSLEFVLSLRRAGVGLFVVDEAHCISEWGHNFRPDYLFLPRAVR
                     DLGNPPVLALTATATPRVRKDILASLKMRSPQVVVTSFNRPNLNYRVIPAKEKKHKLP
                     RILDVIRASPPPGIVYATTRKECEELAAGLRRSGVDAAAYHAGMGSADRANIQERFMT
                     DELAVVVATIAFGMGVDKPNVRFVIHSSVPGSLPAYIQESGRAGRDGEGSECVVLYRG
                     ADLGRRKRLVTLGAAGEREVSSFFSALSGVESDGRVNVPPASLSALGGVDPESAGIVM
                     GSLEESGLISRGYDLWAEVEVRAIEEEPKGLREEVAAVHAALPGSGSVRLPELARKTG
                     LRPAVVQGAIYRMMVDGVAEIIPRGSLVDVRLKTFSLDNDSRRNIARRLKNRANTAYA
                     QIRDVESFANLRTCRREHLLRHFGDMEEVAPCAGCDVCLGETEELPRATGPRAGIPEG
                     QPLVPAFAGSSPADQEGPAVDPALFERLRSWRGEQAKKQRVPAYVVLHNSHLEAISAR
                     KPTTIHELGAIKGVGLRRAARYGDEILALIRGEEAPDIAPPAENGFREHLETAGSLLR
                     SGRGADAVPALARALELGGEEARREVDRLLGPRTDE"
     gene            3240..3761
                     /locus_tag="VN178_00425"
     CDS             3240..3761
                     /locus_tag="VN178_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWS79455.1"
                     /translation="MRLEGKKIAVLVAEGFEDLEYWVTVMRLQEEGAEVVSVGPDTEP
                     VSGKNALGAQADTTADGVDASELDGVVVPGGWAPDKLRRYPEITDLVRTVHERNKTVG
                     IICHGGLVAISAGIVDGARATGSLGIKDDLENAGAIWVNKAAFREGNLVWGRVVPDIP
                     DFCRELVAALAEE"
     gene            4162..4306
                     /locus_tag="VN178_00430"
     tRNA            4162..4306
                     /locus_tag="VN178_00430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4198..4200,aa:Met,seq:cat)
     gene            4384..>5228
                     /locus_tag="VN178_00435"
     CDS             4384..>5228
                     /locus_tag="VN178_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWS79456.1"
                     /translation="MTERMRIATFNLENLDDKPDEEPSLHERIPLMRPQLTRLAADVL
                     CLQEVHGQEKDGEPRRLLALEQLLEGTPYAAYHVAHTKTQGGEAYDERNLVVLSRRDI
                     LGQEQYMNRFCPAPLYKKVTADPPPAEPEPIGWERPILHVRIGLEGDKTLHIFNVHLK
                     SKAPTDIVGQNKDVPDPKPPPFAWRTAYGWAEGYFVSSMKRVGQALEARIIVDKLFDQ
                     DPSALIVVCGDFNADSDDVPVQAIRGDVESTGNPALSQRVLVPCEQSIPESARYSYLY
                     HDKGE"
BASE COUNT          996 a         1594 c         1700 g          938 t
ORIGIN      
        1 gtcgccgtgg tgctcaccat actctcgggc tggtcgtatt tgaaggacac gccgagaatc
       61 ttgtcgatta cctccaagcg agatcagagg ctctaaaagc tgtagaatca cgccccgtat
      121 ggagggcaac gatgacaggc gcctgaggcc ggacgaggtc gaaatgatct tgcgccgcgc
      181 ctctgaactg aactctcgca gggggatgcg tggctcctac gagacacctt ctgtctctcc
      241 cgaggtcctc gtacaggtgg ccgccgcggc cggtatcccc gaacaagacg tgcgccgggc
      301 gatgtgggac attgcttcgg agaggacggc cgagccacgc tcgttcgccc gcaagttcta
      361 cggtcctgcc cgcctcaggg ccgtgcgcga ggtggaacac cccgccgaga ggacccgcga
      421 gtatctcgaa gggttgttgc gcctgcagca gggcctcaag ctaaggcgca agacggaggt
      481 cgggtctttg tgggaccccg gcgacgagct cggagtcgtg aggaggacgc tggacttctc
      541 gggggacagg acgctgctca aggccaagtc ggtcgaactg cgggtcgagg gtttgggcga
      601 agggcgttgc gaggccaacc tgacggccga tctctccaac cagcgcggcg agtacatctc
      661 gctcgccggc atcctcgggg ccacgctggc cgcattgttc atgctcgccg ggatgcagaa
      721 tccgctcttc tttctcggct tccttcccat gctcgtggcg cccgccttcg ggttcagatt
      781 cgcctacagc agggcttccc tggagttgag acgggccctc gacgaactcc tggacgcctc
      841 cgaagagagc cagcctcaag aggaacctca agatcgcaac gatcgtcccc gcggccagat
      901 tcagggcctc gaacccatac caaggttctc ccccaacagg agcaagcggg tagaacccta
      961 gagcttgcaa agagggaacc tccttgcaag ccatcagctt tcagcacttt gttcttgctg
     1021 accgctgata gctgagagct gatagcttat atttaccttc gtgcctcata gagttctaaa
     1081 ggagatcttc ggcttcgatt cgttcaggcc tggccaggag gaggtcataa gggccgttct
     1141 ggagggcagg gacacgctcg ccgtgatgtc caccggcggc ggcaagagcc tctgttacca
     1201 ggtgcccgcg ctgatgcagg agagcctgac ggtgatcgta tcgcccctga tctcactgat
     1261 gaaggaccag gtcgactcac tgcttcagag ctcggtcttc gacgcggcca cgctccactc
     1321 gggactctcg cccgaggagc gttgggaggt cgagcggagg gtgcgcacgg gggaggtgaa
     1381 gatgctctac gtcgccccag agcgtctcag gtccctggag ttcgtcctct cgctccggcg
     1441 cgccggcgtc ggcctgttcg tcgtagacga agcccattgt atctcggagt ggggccacaa
     1501 cttccgcccg gattacctgt tcttgccgcg cgccgtgagg gatctgggta acccccccgt
     1561 cctcgccctt acggcgacgg cgaccccgcg tgtcaggaag gatatcctgg cttccctgaa
     1621 gatgcgctcg ccccaggtgg tcgtgacgag cttcaatcgc ccgaacctga actacagggt
     1681 gattcccgca aaagagaaga aacacaaact gccacgcata ctcgacgtca tacgcgcctc
     1741 accgccgccg gggatcgtct acgccacgac gcgcaaggag tgcgaggaac tcgctgccgg
     1801 cctgcgccgt tccggggtcg acgccgccgc gtaccatgcc gggatgggct cggccgacag
     1861 ggccaacatc caggagcggt tcatgaccga cgagctcgcc gtggtcgtag cgaccatcgc
     1921 tttcgggatg ggggtggaca aaccgaacgt caggttcgtc atccattcga gcgtccctgg
     1981 gagcctaccc gcgtacattc aggagtcggg gcgcgcgggc agggatgggg agggatccga
     2041 atgcgtagta ctgtatcgtg gcgccgacct cggccggcgc aaacggctgg ttaccctggg
     2101 cgcggcggga gagcgcgagg tctcttcctt cttttcggcg ctctccgggg tggagagcga
     2161 cgggagggta aacgtgccgc ccgcttctct ctcggcgctg gggggagtcg accccgagtc
     2221 ggccggcatc gtcatgggga gcctcgaaga gtccggtctt atctcccgcg gctatgacct
     2281 ctgggccgag gtcgaggtgc gtgccatcga agaggagccg aaggggctgc gcgaggaggt
     2341 ggccgcggtt cacgccgcgc tgcctggctc gggctccgtc aggcttcccg agctggcgcg
     2401 caagacgggg ttgcgtcccg ccgtggtgca gggtgccatc tacaggatga tggtcgacgg
     2461 tgtggcggag atcatccccc gcggctctct ggtggacgtg aggctcaaga ccttctcgct
     2521 ggacaacgac agccgccgca acatcgcacg caggctcaag aaccgggcga acacggccta
     2581 cgcccagatc cgggacgtgg agtccttcgc caacctgcgt acctgccgcc gcgagcatct
     2641 cctcaggcac ttcggcgata tggaagaggt cgcaccctgc gcaggatgcg acgtctgcct
     2701 cggcgagacg gaagagctcc cgagagccac cggcccacga gctggcatcc ctgaagggca
     2761 gccgctcgtg cctgcgttcg ccgggtcctc accggcggat caggaaggtc cggccgtgga
     2821 cccggcgctc ttcgagcggc tgaggagttg gaggggtgag caggcgaaaa agcagcgggt
     2881 gcccgcctat gtcgtgctcc acaactcgca cctggaggcg atctcagccc gcaaacccac
     2941 caccatccac gaactggggg ccataaaggg agtaggcctg cgccgcgcgg cccgctacgg
     3001 ggacgagata ctcgccctga tccggggtga ggaggcgccg gatatcgcgc cgcctgcgga
     3061 gaacggcttc agggagcacc tcgagaccgc cggcagcctt ctccggtcgg ggcggggtgc
     3121 cgatgcggtg cccgcgctgg cccgcgccct ggagctcggc ggcgaagaag cgcggcgcga
     3181 ggtggaccgg ctactaggac cgcgcacgga cgagtgagga ccgtacgaga ggagtctcta
     3241 tgagattgga agggaagaag atcgctgtac tcgtggccga gggtttcgag gatctcgagt
     3301 actgggtaac cgtgatgcgc ctgcaggagg agggggccga ggtcgtctcc gtcggacctg
     3361 acaccgaacc ggtcagtggc aagaacgcgc tgggggcgca ggctgacacc acggcggacg
     3421 gtgtcgacgc ctcggaattg gacggcgtcg tggtgcctgg cggctgggcg ccggacaagc
     3481 tcaggcgcta tcccgagatt acggacctcg tcaggaccgt gcacgaaagg aacaagaccg
     3541 tcggcatcat ctgccacggc gggctcgttg cgatctctgc cggcatagtc gatggggcga
     3601 gggccacggg ctcgctgggg atcaaagatg acctggagaa cgccggcgcg atctgggtaa
     3661 acaaggcggc gttccgtgag ggcaacctgg tctggggccg cgtggtcccc gacatccccg
     3721 acttctgccg cgagctcgtc gccgccctgg cagaggaatg aggcgtcttc ggcacaccag
     3781 cccgtacacc aatgggaagc ggggcggtag gggtgagcaa gcgtgccaac gtttaggtaa
     3841 ccgaagtcga gaaaattgtg atcgctacta attccctcgc agttaatgac tcaccgatac
     3901 accgattatc ggcgaggtac gatgccgagg tcactactca tggtcactgg ggacgttcgc
     3961 cgactttact ttcgacccta tccctgcgtt cctacgatgg gaagctgaag ccaggcaatt
     4021 gaccgcgttg agtggtgtgt tgaggttagg tggttgatta gctggccgaa aggagaactg
     4081 cgggcagttc tgtaataact aggtacgagg gtcctcacta cgatatcctt cagtaaggac
     4141 agccagagtc aacaccatct cggaggtggg tggagcagtc tggtagctcg tcggcctcat
     4201 gacccgaagg tagcaggtac aggcttcgtc gagaaaagtc tgctgtctcc tgtcacttgg
     4261 aagaatttta tgcagatttg cgtattattc ttcgtattca cctccaattc atctgtaatt
     4321 catcgccatg gcgtagcgtg gccccttgac ggtatcccga gagaccaagg aggcgcgagc
     4381 accgtgacag agaggatgcg tatcgctacg ttcaacctgg agaacctcga cgacaagccg
     4441 gacgaagagc cctccctgca tgagcgcata cccctcatgc gtccgcagct cacgaggctc
     4501 gccgccgacg tcttgtgcct ccaggaggtc cacggtcaag agaaggacgg cgagccccgt
     4561 cgcctgctgg ccttggagca gctactcgag ggaacgcctt acgctgccta tcacgtggcg
     4621 cacaccaaga ctcagggtgg cgaagcctac gatgaacgca acctcgtcgt attgagtcgc
     4681 cgtgatatcc tcggccaaga gcagtacatg aatcgcttct gccccgctcc tctatataag
     4741 aaggtgactg ccgacccgcc gcccgccgag ccggagccga tagggtggga gagacctatc
     4801 ttgcacgtaa ggatcggcct cgaaggagac aagactttac acatattcaa cgtgcacctg
     4861 aagtccaagg cgcccacgga catagttggc cagaataagg atgttcccga ccccaagcct
     4921 cctcccttcg cctggaggac ggcctacggc tgggccgaag gctacttcgt ctcttctatg
     4981 aagcgcgtag ggcaagccct tgaggcccgc ataatcgtgg ataagctctt cgatcaggat
     5041 ccgagcgccc tcattgtcgt ctgcggggac ttcaacgccg actctgacga cgtacccgtg
     5101 caggctatca ggggcgacgt ggagagcact ggcaatccag ccctctcgca gcgcgtcctt
     5161 gtgccctgcg agcagagcat tcccgagtct gcccgctact cgtacctcta tcacgacaaa
     5221 ggagagat
//
LOCUS       DATMVF010000013         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_598271, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000013 DATMVF010000000
VERSION     DATMVF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598271"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VN178_00440"
     CDS             <1..1077
                     /locus_tag="VN178_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS79457.1"
                     /translation="EPVDIDLDALSDYINGKRVLVTGAGGSIGKELSRQISRLGPAKL
                     ILLDRDESGLYYLNTELRREDFYDTEVFVGDVTHSERVSFAFETFRPQLVFHAAAYKH
                     VPMMEQQATEAIVNNVLGTLNVARAAGAYGTSKFINVSTDKAVSPVNVMGATKRLAEM
                     VVRELSLEYPETIYASVRFGNVLGSRGSVVPTFRQQIEAGGPVTVTHPEMIRYFMTIP
                     EAVSLILQAGAMAESYGTYVLEMGRPVAITDLARKMIEIMGAPNVTIKFVGLRPGEKL
                     KEELSEEGEQRDTTAHQMVFRLSSENMSPPGDSNLSEIIDAMVFHARGQEGGRALQLL
                     RRAVPNYSAADLPEITESRIDYPL"
     gene            complement(1313..1522)
                     /locus_tag="VN178_00445"
     CDS             complement(1313..1522)
                     /locus_tag="VN178_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79458.1"
                     /translation="MKLSRRTANILLAIGVYMLLTWGTRVFTFLSEFRAGTLVAPAVH
                     LSLVVIGLAIGIYLAYLGIKGRRVS"
     gene            1659..2774
                     /locus_tag="VN178_00450"
     CDS             1659..2774
                     /locus_tag="VN178_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HWS79459.1"
                     /translation="MAELVRRNLDEVKALSRDEAVEIMRQAGIVGAGGGGFPTYFKWK
                     NPQPNLIVNCTESEPGYWADKVLHRDYFDEFLELFDVMKTIFGIERVIMGVHFKDEEW
                     FSRYDENTDGLYEVVHVPDKYSMGEEKTLIKNIFYEDKGKWVPRRVELPDGNKRPGLP
                     LDAGHIVNNTETLLNIYNAFFLGKPVTTKYLQVFGPDLELELFEAPLGSSATELLKLS
                     GVDVETEAGNLTVIDGGPYLNEMGIESLGESDAYVRRTTNGFLVIPKGVASKEYADIK
                     TRRPEGVTSLVGKVSGISIPLGGRFLKPATALVSEGDEVTFEQKIGEPVDEGFSIGVW
                     SGMDGNITAIEEDIVQISGGAMPLEEAEAETEAEAAR"
     gene            complement(2983..>3193)
                     /locus_tag="VN178_00455"
     CDS             complement(2983..>3193)
                     /locus_tag="VN178_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79460.1"
                     /translation="ESSPANALQILRSHNISIRNTDAEIDEDRVRYNLQVRIHPTADV
                     HAVLAELSGLPEVERISLTGFKDYE"
BASE COUNT          663 a          961 c          982 g          587 t
ORIGIN      
        1 gagcccgtgg acatagacct cgacgccctc tccgactaca tcaacggcaa acgggtgctc
       61 gtgaccggcg cggggggctc catcggcaag gagctctcgc gccagatctc acgcctcggc
      121 ccggcgaaac taatcctgct cgaccgggac gagagcggcc tgtactacct gaacacggag
      181 ctgaggcgtg aggacttcta cgacacggag gtcttcgtcg gcgacgtcac gcactcggag
      241 cgcgtcagct tcgccttcga gactttcagg ccacaactcg tcttccacgc ggccgcctac
      301 aaacacgtcc ccatgatgga gcaacaggcg accgaggcca tcgtcaacaa cgtcctcggc
      361 accctcaacg tcgcccgtgc cgcgggagcc tacgggacga gcaagttcat caacgtctcg
      421 accgacaagg ccgtaagtcc cgtcaacgtg atgggcgcga ccaaacgcct ggccgagatg
      481 gtcgtcaggg aactctccct ggagtacccc gagacgatct acgcctccgt ccgcttcggg
      541 aacgtgctgg gcagccgcgg ctccgtcgtt ccgaccttcc gccagcagat agaggccgga
      601 ggccccgtaa cggtcacgca ccccgaaatg atccggtact tcatgaccat ccccgaggcg
      661 gtctccctta tcctgcaggc cggggcgatg gccgaaagtt acggcaccta cgtcctcgag
      721 atgggccgtc ccgtcgccat caccgacctg gcacgcaaga tgatcgagat tatgggcgcg
      781 ccgaacgtca cgataaagtt cgtcggcctg cgccccggcg agaagctgaa agaggagctc
      841 tcggaggaag gggaacaaag ggacacgacg gcccatcaga tggtcttcag gctctcctca
      901 gagaacatga gcccgcccgg tgactcgaac ctctccgaaa taatcgatgc catggtcttt
      961 cacgcccgcg gccaggaggg tggaagggcc ctccaactcc tgcgccgcgc cgtacccaac
     1021 tactcggccg cggacctgcc cgaaatcacg gaaagccgta tcgactaccc actgtagtgg
     1081 gtagcacgct ccggcttcgc ctccgcttgt cagcatttca gcacgtttcg ggccttcggc
     1141 cctcactcgt cagcaggtca gcaagagtgt gcagagcgca aggctcgttc tgaaagcggc
     1201 gtctccgctt gtaggatctt gctgctcgac cgtaagcgag gccttcagag gaaaaagctg
     1261 acatgctgac aagcgaaggc taaaagcctg agcgtgctga agtgctgact tgctaactca
     1321 cccttcggcc cttgatgccg agatacgcca gatagatgcc gatggcgagc ccgatcacga
     1381 cgagcgagag gtgtacggca ggggcgacca gcgtccccgc acggaactca gagaggaagg
     1441 taaagaccct cgtaccccag gtgaggagca tgtagacgcc tatcgcaagt aggatgttcg
     1501 cggtgcgcct gctgagcttc atgggcgtca gggagtgtac cagacccgcc tctcccgaac
     1561 tagcaacata aaggcgcaaa gggtatactg gctgtaatag ttggacggag attgtaacag
     1621 taagtatctg gtgtatagag tattcggagg gaaggatctt ggctgagtta gtgcgacgca
     1681 atctcgatga ggtaaaggcg ctcagcaggg atgaggctgt ggagatcatg cgccaggccg
     1741 gcatcgtcgg ggccggaggc ggaggtttcc ctacttactt caagtggaag aacccgcaac
     1801 cgaacctgat cgtaaactgc accgagtcgg agcctggtta ttgggccgac aaggtgctac
     1861 acagggacta cttcgacgag tttctcgagc tcttcgacgt gatgaagacg atcttcggga
     1921 tcgagcgcgt gatcatgggc gtgcacttca aggacgagga gtggttctcc cgctacgacg
     1981 agaacacgga cggtctctac gaggtcgtcc acgtgcccga caagtactcg atgggtgagg
     2041 agaagacgct catcaagaac atcttctacg aggacaaggg aaagtgggtg ccgcgccgcg
     2101 tggagctgcc cgacggcaac aagcgccccg gccttccgct cgacgccggg cacatcgtca
     2161 acaacaccga gacgctcctg aacatctaca acgcgttctt cctgggtaag ccggtgacga
     2221 ccaaatacct ccaggtcttc ggccccgacc tagagctcga gctcttcgag gcgccgctcg
     2281 ggtcttccgc cacggagctc ttgaagctct ctggcgtgga cgtcgagacc gaagccggca
     2341 atctcaccgt catcgacggc gggccctacc tgaacgagat ggggatagag tcgctgggcg
     2401 agagtgacgc ctacgtcagg cgcaccacca acggcttcct cgtcatcccc aagggcgtcg
     2461 cctccaagga gtacgcggac atcaagacca ggcgtcccga gggcgtcacc tcgctggtgg
     2521 gcaaggtcag cgggattagc atcccgctcg gcggcaggtt cctcaagcct gctaccgcgc
     2581 tcgtctccga gggggacgag gtcaccttcg agcagaagat cggcgagcct gtcgacgaag
     2641 gtttttccat cggagtctgg tccggtatgg acggcaacat caccgccatc gaggaggaca
     2701 tcgtccagat ctccggcggc gccatgcctc tggaggaagc cgaggccgag acggaggccg
     2761 aggcggcaag gtaggtttct ttataggcta cgggtttcag gcatcagggg gccggggcgt
     2821 aaatcccggc cctttcttac tgtggttctc gattagaatg agcctggagg cgtcggcccg
     2881 ttttctccca tacatcgaat ctctttgacc cctacctgaa gcctgtagcc tgatgcctgc
     2941 ggtttgcaaa caaagggctg cgccttctgt ttgcaaaccg ctttattcgt agtccttgaa
     3001 gcccgtaagt gagatgcgct ctacttcggg gaggccggat agctcggcga ggacggcatg
     3061 aacgtcggcg gtcgggtgta tgcgcacctg aaggttgtag cggacgcgat cctcgtctat
     3121 ctcggcgtcc gtgttgcgga tgctgatgtt gtggctcctg aggatttgga gtgcgttcgc
     3181 ggggctactt tca
//
LOCUS       DATMVF010000014         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_276992, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000014 DATMVF010000000
VERSION     DATMVF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276992"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VN178_00460"
     CDS             <1..1512
                     /locus_tag="VN178_00460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS79461.1"
                     /translation="AERDTLSELVVIGGGPAGVTAALRARELGAEVVLIERDKLGGTC
                     TNDGCVPTRALAKAARLVRDAEQFADYGLRGPAPEVDFPGVLLAAQRVVEEVHGKKRI
                     RDRLQEAGVDVVDGAGEVRFADPHTVRLENGVPGGTKAEVRGVRFVVCAGGHARRLGF
                     PGSEHALTHSDVWNMESLPGSVAIIGAAATGCQLASVFAAFGSRVRLLDLAPRILPGE
                     DEAVSRAVGESFRRRGIHIETGIGGVNGIERVSGGPEDLILHYDTPDGEREAAVGAVM
                     LSVGWPGNVDALGLEAAGVATDRGHIVVDDTLQTSVPHIFAAGDITGRVMLVQSATYE
                     GSLAAEQAVLGAERSQSHEIVPHGGFTDPEYASVGLTEEAALAWHDCVVSSVPYTDLD
                     RSVIDGHQEGFCKLIVDRASRRILGAHIVGEQAVEVVQLVATAMRGGMPVEELADIEL
                     AYPTFTAIVGLAARRAARELGVAIGCRGWSPRSHLGVAEWELGKDRDKAALVL"
     gene            complement(1601..1894)
                     /locus_tag="VN178_00465"
     CDS             complement(1601..1894)
                     /locus_tag="VN178_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79462.1"
                     /translation="MVLDGLRAQDRQGEVAHRSEGSRAVVVAVSSYSPDLNPIEEAFS
                     KTKNIVRKAGARTRQASVEAIAKAISSLTLEDVAGWFAHCGYYPQDQYSCTPL"
BASE COUNT          481 a          815 c          856 g          459 t
ORIGIN      
        1 gcagaaaggg acacattgag cgagttagtg gtaataggtg gagggccggc gggggtaacc
       61 gcagccctga gggcccggga gctgggggcg gaggtcgtcc tgatcgagcg cgacaagctc
      121 ggcggaacct gcaccaacga cggctgcgtc ccgacccggg cgctcgccaa ggccgcccgg
      181 ctcgtgcggg acgcggagca gttcgccgat tatggcctcc ggggcccggc gccggaagta
      241 gacttccccg gggtgctcct cgcggcccag cgggtcgtgg aggaggtaca cggcaagaaa
      301 cgtatccggg atcggctgca ggaggcgggg gtcgacgtcg tagacggagc tggcgaggta
      361 cgtttcgcgg acccccacac cgttcggctc gagaacggcg tccctggggg aacgaaggcc
      421 gaggtccggg gcgtccgctt cgtcgtctgc gccgggggac acgcccggag actcggattc
      481 cctggctccg agcacgccct cacccacagc gacgtgtgga acatggagtc gctgccggga
      541 tcggtcgcga tcatcggtgc cgcggcgaca ggttgccagt tggcctccgt cttcgcggct
      601 ttcgggtcca gggtgcggct tctggatctc gccccgagga tactccccgg ggaggacgag
      661 gccgtctccc gggccgtcgg cgagagcttc cggcggcgcg gcatacacat agagaccggg
      721 atcggcgggg taaacggcat agagagggtc tccgggggac cggaggacct aatcctacac
      781 tatgacaccc cggacgggga gcgggaggcc gcggtgggcg ccgttatgct ctccgtgggc
      841 tggcccggca acgtggacgc gctcgggctc gaggcggctg gcgtggcgac cgacaggggc
      901 cacatagtcg ttgacgatac gctacagacc agcgtgcctc acatcttcgc cgcgggcgac
      961 ataaccggca gggtcatgct ggtccagagc gcgacctacg agggcagcct cgccgccgaa
     1021 caggccgtgc tcggtgccga gaggtcgcag agccacgaga tagtacccca cggcggattc
     1081 actgaccccg agtacgcaag cgtcgggctc acggaggagg cggcgctggc ctggcatgac
     1141 tgcgtggtct cctctgtccc ctatacggat ctcgaccgca gcgtcatcga cggccaccag
     1201 gagggattct gcaagctgat agtggaccgc gcctcgcggc gcatcctcgg cgcccacata
     1261 gtcggcgaac aggcggtgga ggtcgtccaa ctcgtggcga cggcgatgcg tggcgggatg
     1321 ccggtagaag agctcgcgga catagagctc gcctacccga cgttcaccgc gatagtcggg
     1381 ctcgcggcca ggcgtgcggc ccgggaactc ggcgtcgcga tcggctgccg tgggtggagc
     1441 ccccgaagcc acctcggggt ggccgagtgg gagctcggca aggaccgcga taaagctgcc
     1501 ctcgtgctct aggagaaccc tgccaggtag cctgaacacg ccacttacga tcaccaccgt
     1561 ggataaacgc ccctaaaaac ccgcagccga aacctgagac ctacagcggt gtacatgagt
     1621 attgatcctg tgggtagtag ccgcagtggg cgaaccatcc cgccacgtct tcgagcgtga
     1681 gcgacgagat cgccttggct atggcttcga ccgacgcttg gcgagtgcgt gctcctgcct
     1741 tcctgacgat gttctttgtc ttcgagaacg cttcctctat ggggttgagg tcaggcgagt
     1801 aagacgagac ggcaacaaca acagctcggc tcccctcgct tcgatgagct acctcacctt
     1861 gtcggtcctg tgcgcgtagc ccatctagca ccacctgccc ctcccctagc gtggggggca
     1921 ggtggtgctc cacgtaggcc tcgaaaatac ggacctccca aagccgagcg ggatggcaaa
     1981 cccgacgagc agcagcaaat gtttggggag aagtaggcac tctgggatcc ggatctatat
     2041 cgcactttga cggacttgtg caccctagac tcctagccca tcgcaccagc tctcatcctc
     2101 gaaaagagtg gcggaccgcc tttagagaac ccttgcagtt catcgtcatc gccgtcatga
     2161 gaggtatttt tcccgatacc tatgggcttt tctacgtgac gatttattcc tggcgtgttc
     2221 ggggaccgtc acgtcaccgt catggtgagc gtcgccgaag aaacgtctta cctaagcggc
     2281 ttcctcgcag gcgtgacggt gatgacgctc atgacggtcc ttacgggcta attctagaca
     2341 gaggatgcgc cgctacttcg caggctctcc taccctgtca atctaaccca acgaacccga
     2401 cgcgcagagc aagtagaccc ttacctatgc gatcttcgag gctactagcg gcgtcgggtt
     2461 tgcctaacct ctcctatacc cgacgctgct acccgacgac gaaccacctt tgtcgtcggg
     2521 ttatcgagag cttcgtcggg ttctagttgg taacccgacg acaacaaaca ccttacttat
     2581 gccggatact gcggccatcg tcgggttcgt c
//
LOCUS       DATMVF010000015         7855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_230922, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000015 DATMVF010000000
VERSION     DATMVF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230922"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VN178_00470"
     CDS             <1..377
                     /locus_tag="VN178_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79463.1"
                     /translation="ERPPRQPPTVEVLHSNERADLTSVFGTSTPPSGPSGALRRLAFR
                     YSESSYGHWLPLVLADRLSVVGGVLGDLKQGRVPNVFAERGWKAQWEHNRSDLFRRIL
                     LRVLIVSAIVAYFRSRRGNSYR"
     gene            complement(506..1174)
                     /locus_tag="VN178_00475"
     CDS             complement(506..1174)
                     /locus_tag="VN178_00475"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWS79464.1"
                     /translation="MARALDVVGERWTLLLVRELSTGPKRFKDLLDGLSGIGTTLLTA
                     RLKDLEANGILRRTTLPPPAGSKVYELTDLGRALEPVVIALSRWGLKLLDAPRPEEET
                     RPAWAMVALQSSLDLKAASGHKETYEFRVDGGLFHVRVEGDGAEVRQGPAADPDLIIS
                     GDTETLLGVAAGRLTLAEAVEAGAIATEGDRDSLARCLAMLGSPDNDSGKSVRDDIRA
                     QQAR"
     gene            1348..2187
                     /locus_tag="VN178_00480"
     CDS             1348..2187
                     /locus_tag="VN178_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HWS79465.1"
                     /translation="MMHYTEERVATREAETPELLSSYFARIDNGALLTHAQEVDLSRR
                     ARAGDAKARTRLVEKNLRLVVSVAKKYRGYGVPFEDLIQEGNIGLMKAVEKFDPDRGF
                     RFSTYATWWVRQAIGRAVSDKGRTIRVPVHMGEKTRKMARTYNGLSAELGREPTDEEV
                     AGHLGWTAEKVREVKSTLPDATSLDKPLGSEEDASELGSLVEDQGSSDVSEAVVRDME
                     VGWLGEAISRLPQRHRRVLVKRYGLDEEQTATLAQLAEELGVSKERVRQLQREAEHML
                     RAA"
     gene            complement(2346..2723)
                     /locus_tag="VN178_00485"
     CDS             complement(2346..2723)
                     /locus_tag="VN178_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79466.1"
                     /translation="MAKREPDRGATERSSEPGHGPQTLAQLQATGAEVVGSDGERLGD
                     LKEVGDADFLVGRTLRRDLRLPVNRVREVTSENEIVLDLSAAEAKELGEKETLGEAAP
                     AAGSMAENPGKEEKGSWGLRKGE"
     gene            complement(3037..3534)
                     /gene="mug"
                     /locus_tag="VN178_00490"
     CDS             complement(3037..3534)
                     /gene="mug"
                     /locus_tag="VN178_00490"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HWS79467.1"
                     /translation="MAVDLLRPGLTLVFCGYNPSLASGRSGNHYAHPGNRFWRVLAAS
                     GVTERLYEPHEDETLLGLGIGFTNLCSRPTRRADELTREEIRSGALALRLKLEQFRPR
                     VVAYTGIGIYKWFRATSKAEWGVQEVSAVGGVTDIVVPSPSGLNRINFEELVDHYRAL
                     IPFLC"
     gene            complement(3919..5037)
                     /locus_tag="VN178_00495"
     CDS             complement(3919..5037)
                     /locus_tag="VN178_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWS79468.1"
                     /translation="MKIREVSTVGLKGATPEGGWSEELKPDDVVHTLVTVHTDEGLVG
                     YGSVFTSEALVRAALEVLEPLYAGETALEPERVSEKLHQNTFWQGRGGAITHTISGVD
                     IALWDILGKVTGQPVGRLLGGRYRERIMPYASLLMQEPGPLSERLVSLREQGFQAFKV
                     GWGPFGRVSDRLDEAIVKAAREAIGDQAILAVDPGGSDAFWPRGYKWALRTSCMLAEY
                     GIAWFEEPLVPDALDDFVALRRAAPVPISGGEVLTRRQSFEPYLRAGAFDVVQPDVTK
                     VGGISEERRIGWMAEENGARMIPHGWNTAVGLAADLQLASALPKTDLVEYVTGSAYVD
                     DIAANGWKLDAEGLLPIPDAPGLGLELDADAVSKYSGG"
     gene            complement(5625..5936)
                     /locus_tag="VN178_00500"
     CDS             complement(5625..5936)
                     /locus_tag="VN178_00500"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWS79469.1"
                     /translation="MRERLADTYWLATVRPYGLLHLVPLLAVWVGGAMHVVAITTFRK
                     ATNLAHDAHCVIAARREALDLVVEGTAAKVSEEARLHRGAEVYMNKYGWPVTIRNGAL
                     E"
     gene            complement(6001..7434)
                     /locus_tag="VN178_00505"
     CDS             complement(6001..7434)
                     /locus_tag="VN178_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS79470.1"
                     /translation="MPDNRQHALELSHAEPLDEAAMRTFYSNVRGQVILPDDAIYKEA
                     RTVFYGGFDRHPAAIVRVKDASDIGRVVSLARESGLELAIKSGGHSLAGHSISEGGIV
                     LDLSGMKNLELDTEGRTAWAQTGLTAGEYTAAVGAHGLATGFGDTGSVGIGGITLGGG
                     VGYLVRKHGLTIDDVLAAEIVTADGELVYVDAERHPDLFWAIRGGGGNFGVVSSFRYK
                     LHEVDTIVGGMLVLPATPEVIASFVAEAEAAPEKLSTIANVMVAPPMPFLPAEAHGKL
                     VITALLCYAGEVDEGERVVAPFRALATPLIDTVGPKPYPEMYEPMGPGPDEEVARSLF
                     IDSFDGSAAETIVEYLQASSAPMAVAQLRVLGGAMARVPVEATAFAHRRRRIMVALGA
                     VYERREETAEHEDWVTNFAAALKQDDTGVYVNFLGDEGEARVRKAYPGSTWERLVEIK
                     GRYDPTNLFRLNQNIPPASGQKEAGHS"
BASE COUNT         1473 a         2524 c         2361 g         1497 t
ORIGIN      
        1 gggagcgccc gccgcggcag ccgccgaccg tcgaggtcct gcactccaac gagcgagctg
       61 acctcacctc cgtgttcggt acatcgactc cgccctcggg accgagtggc gcgctccgcc
      121 gcctggcttt caggtacagc gaatctagct acggccactg gctgcccctg gtgctggccg
      181 accgtctgag cgttgtggga ggcgtccttg gcgacctcaa gcaaggccgc gtccccaacg
      241 tcttcgcgga acggggctgg aaggcccagt gggaacacaa caggtcggat ctcttcaggc
      301 ggattctcct cagagtacta atagtttccg ccattgttgc ctactttagg agccgaaggg
      361 gcaattcgta tcggtagcaa tgatcgtcgt agtgatcatg cggcgctgac catcgtcacc
      421 gtatccgggg cacgagcaag gttcggccga agaagtgagg gcgacgcatt cggtacgcgg
      481 cggtacggta gagattagct aaggttcagc gagcctgctg agcacggatg tcgtcgcgca
      541 ccgatttccc gctatcgttg tcaggcgatc cgagcatcgc aaggcagcga gccaaggagt
      601 cccgatcgcc ttcggtcgcg atcgcacccg cttcgactgc ttcggcgagc gtcaatctgc
      661 ccgcggcaac gccgaggagg gtctccgtgt ccccgctgat tatcaggtct ggatcggcgg
      721 cggggccctg ccggacttcg gccccgtccc cttcgacccg cacgtggaat aaccccccgt
      781 caacgcggaa ctcgtaggtc tctttgtgcc cagacgccgc tttcagatcg agagacgatt
      841 gcagcgccac catcgcccat gccgggcggg tttcttcttc tggacgcggg gcgtccagga
      901 gcttcaatcc ccaacgggag agggctatga ccaccggttc cagtgcacgt cccaagtcag
      961 tgagctcgta caccttcgat cctgccggtg gcggcaaggt cgtccggcgt aagatcccat
     1021 tcgcttccaa atctttgagc cgggcggtca agagggtcgt cccgatgccg gataacccgt
     1081 ccaagaggtc cttgaaccgc ttgggtccgg ttgatagctc ccgcaccagc aacaacgtcc
     1141 atcgctcacc gacgacatcg agcgcccggg ccaccgcgca gtactgatca taggtgcgcc
     1201 tactcacaag agcgagtgta cacgatttca caatgcagta cattttcaga tcatgataac
     1261 tttcaaaagc tgtagtagat actgtataat ttgatcgaga taggacatgc gtcgagaaga
     1321 gatcatctca caaggggaga aagccggatg atgcattaca ccgaggaacg cgtggccacc
     1381 agggaggcgg agacgccgga gcttctttcg agctacttcg ctcgcatcga caacggcgca
     1441 ttgctgacac acgcgcaaga ggttgacctc tccaggcggg ctagggcagg ggacgccaag
     1501 gcgcgcacga ggctcgtcga gaagaacttg aggctcgtgg tatcggtcgc caagaagtac
     1561 agagggtatg gtgtgccctt cgaggatctc atccaggagg gcaacatagg attgatgaag
     1621 gccgtcgaga agttcgaccc cgacaggggc ttcaggttct ccacgtatgc tacgtggtgg
     1681 gtaaggcaag ctatcggaag ggcagtgtcg gacaagggtc gcaccataag ggtgcccgtg
     1741 cacatgggcg agaagacgcg caagatggcc cgcacctaca acgggctctc cgccgagctt
     1801 ggccgcgagc cgaccgacga agaggtcgcg gggcacctcg ggtggacagc cgagaaggta
     1861 agggaagtca agagcacgct gcccgatgcc acgagcctcg ataaacccct cggctcggag
     1921 gaagatgcct ccgaactggg cagcctcgtg gaggaccagg gatcttccga cgtttccgaa
     1981 gcggtcgtcc gcgatatgga ggtaggttgg ctaggagagg ccatatcgcg gctaccgcag
     2041 cgccatcggc gcgtgctggt caagcgctac ggcctcgacg aggaacagac tgcgaccctc
     2101 gcgcagctgg ctgaggagct gggggtgtcg aaggagcggg taaggcaact tcagcgcgag
     2161 gccgaacata tgctgagggc ggcttaagcc ggagcgaccc atccgcgctt caggagtccc
     2221 tcaagtcgtt cccattctcg ccttcgtgca accaaccacc aagaaggcgc taacttcgag
     2281 aaaggcaagc tagtaaaatt ctgattgggc ttaggcgagg gttcggttga attggtgagg
     2341 ccagcttact ctccctttcg gagtccccaa gatccctttt cctctttccc gggattctcc
     2401 gccatactac ccgccgcagg agcagcttcg cccagcgtct ctttttcgcc cagttctttg
     2461 gcctcggcgg ccgacaggtc taggacgatc tcgttttcgg aggtgacttc cctcacgcga
     2521 tttaccggca ggcgtaggtc gcgccgaagc gtccgtccga cgaggaaatc ggcatcgcct
     2581 acttccttta gatcgcctag cctttcaccg tcggagccta ccacctcggc gcccgtggct
     2641 tgcagttggg cgagcgtctg gggaccgtgc cccggttcgg agcttcgctc cgtcgctccc
     2701 ctatcgggtt cgcgtttggc cattgcctca tcacctccgc tgtcgtctcg tttactcaag
     2761 ccaaagatgc ccgcatcggt gagatcttaa gcgggacagg gcgaggctta cctagaaatc
     2821 cttgcttctt cttgtgcggt gaacctacga gagtaggcta tttgccagat gtccagcaga
     2881 cgaaagctat gagcgtcacc cgcgagagat caagatccac gccctcctgg ccgaaggtag
     2941 ctctgagttt agatgcgtga gagcttaaca tcgcaggaga tagaagcacc ggtcatcaat
     3001 acactgacct ctaatcgggg catgtgggac tacccactaa catagaaagg gtataagcgc
     3061 acggtagtgg tcgaccagct cttcgaagtt aatacgattg agacccgagg gcgacggcac
     3121 gactatgtcg gtcacccccc caacggccga gacctcttgc acgccccatt ctgcctttga
     3181 ggtcgcccga aaccatttgt agatgccgat gccagtgtag gcgacgaccc gaggtcggaa
     3241 ctgctctagc ttcagtcgta gcgcgagagc acctgacctg atttcctcgc gtgtcagctc
     3301 atcagccctt cgggtaggtc tagagcagag attggtaaaa ccaataccta aaccgaggag
     3361 cgtctcatcc tcatgaggtt cgtacagccg ttcagtgaca ccagatgctg cgagaacgcg
     3421 ccagaagcgg ttgcctgggt gagcgtagtg gtttccgctg cgccccgacg caagtgaagg
     3481 gttatagcca cagaaaacga gcgtcagtcc gggtctgagg agatccacag ccattatcac
     3541 agtctacagg tacggcttct cccttatctg aacgctgaga aggttatcgg gcactcctcc
     3601 tcagccatcg ttacgcgtcc tcggtagtgc ctgaaagtgc caagaatggg atggacaaag
     3661 gtcggaaccg tgactgcagg ggcttcagac accgtactgt tcggcctact ttggccccga
     3721 tataccggcc gattccacgc tcggagcggc gctcaaaccc cttttcggac cgtttccagg
     3781 cgaccttcag gggctgtgga gttgcactat tggatcagcg ccctcggtca gcaaggcacg
     3841 gaagcgaagc ctgggcgact gggtgttttg agactcacgc atccctctcg acattaccgt
     3901 cgtcatccga ggccctcctc acccgccgga gtacttggag acggcgtccg cgtccaactc
     3961 caggcccagt cccggcgcgt cggggatcgg gaggaggccc tcggcgtcaa gcttccaacc
     4021 gttggcggcg atgtcatcca cgtaggcgga gccggtgacg tactcgacga ggtcggtctt
     4081 gggcaaggcg ctggcgagct gcaggtcggc ggcgaggccg actgcagtgt tccacccgtg
     4141 cgggatcatg cgggcgccgt tctcctcggc catccagcct atgcgccgct cctcgcttat
     4201 tccgcccacc ttggtgacat ccggctgcac gacgtcgaag gcgccggccc tcaggtaagg
     4261 ctcgaaggac tgcctccggg tcaggacctc cccgccggag atcggcacgg gcgcggcgcg
     4321 cctcagggcc acgaagtcgt ccaacgcgtc gggcacaagc ggctcctcga accaggcgat
     4381 gccgtactcc gccagcatgc aagaggtcct aagcgcccac ttgtaccctc ggggccagaa
     4441 tgcgtcgcta ccaccagggt ccaccgccag gatcgcctgg tccccgatgg cctccctggc
     4501 ggccttcacg atcgcctcgt cgagcctgtc gctcacccga ccgaacggtc cccagcccac
     4561 cttaaacgcc tgaaagcctt gctccctaag ggagacgagg cgttccgaga ggggtcccgg
     4621 ctcctgcatc agcaaggagg cgtagggcat gatcctctca cgatacctgc ccccgagcag
     4681 gcgcccgacc ggctgtccgg tgaccttccc caagatgtcc cacagggcta tgtccacccc
     4741 gcttatcgtg tgggtgatcg cccccccgcg gccttgccag aaggtgttct ggtgcagctt
     4801 ctccgagacg cgctcgggct cgagggcggt ctcgcccgcg tacagcggct ccaacacctc
     4861 gagggcggca cgcaccagcg cctcgctcgt gaagacgctg ccgtagccga cgagtccctc
     4921 gtcggtgtgg acggtcacca gggtatgaac aacatcgtcg ggtttcagct cctcgctcca
     4981 gccgccctcc ggggtcgctc ccttgaggcc gacggtgctt acctcccgta tcttcatgcg
     5041 cgtgtccgac cctccttgcg tcactcgtcg cccgaaaacg ggcaccttcc gaatccgcgt
     5101 gcccgcgcga gtcaggatgg ccagtgcgtc cgaatcaaca tccgacttcg gattacacaa
     5161 acgccccgac tcgcgtaacc accagcctct ctaccatacc ctagcgcgat ccgctatccg
     5221 agcgtgagag acaagtaccg attagagcga ggagcgccgg cgccgagtag acggtgcgta
     5281 ccccgttcca gaagttcccc ggactttcgt actctgccct gacctgcgcg agtgcttaca
     5341 catcatcatt atttcgaaac tacatacact ctaggccaag gtttcggcgc tctctgccgg
     5401 gatgtaggtg ggcctcgcgg gcacggacgg cacgccgcgc tccgagccca cagctgcgaa
     5461 gtcgcgaccg caaagggaaa agctgaaata ctgcccggac gagtctgccc ctatcgtctc
     5521 cctggcctcg cagcggaaca gttcagcgta gaaagttacc gccctctccg gatcggggag
     5581 aatcgcagca acccagaggg taccccgttc ggtacccgtc gcgctcactc caaggcgccg
     5641 ttgcgaattg tcactggcca cccgtacttg ttcatgtaca cctcggcccc gcgatggagc
     5701 ctggcttctt cgctaacctt cgcggccgtg ccttcgacca ccagatccag agcttccctc
     5761 ctagccgcga tgacgcagtg cgcgtcgtgc gccaggttcg tggctttgcg gaaggtcgtg
     5821 attgcgacga cgtgcattgc gcctccaacc cagacggcca gtaatggtac taggtgtagc
     5881 agcccgtacg ggcgaaccgt agccagccag tacgtatccg ccaggcgctc gcgcacctcc
     5941 gcccacggcg tcggggtcgt atcctcggca aggtacggct gctcgaccgc cggtctcttc
     6001 tcagctatgg cccgcctcct tctgcccgct cgccggcggg atattctggt tgagtcggaa
     6061 gaggttcgtt ggatcgtagc ggcccttgat ctcgacgagc ctctcccagg tcgagccagg
     6121 ataggctttg cggacccgcg cttcgccctc gtcgccgagg aagttgacgt acacgcccgt
     6181 gtcgtcttgc ttcagcgccg ccgcgaagtt tgtgacccag tcctcatgct ccgcggtctc
     6241 ctctcggcgt tcgtataccg cgccgagggc gaccatgatc ctgcgccgcc ggtgggcgaa
     6301 ggcggtagcc tcgacgggta cgcgggccat cgccccaccc agcacccgga gctgggccac
     6361 ggccatcggc gcactcgagg cctggaggta ctcgacgatc gtctcagccg cgcttccgtc
     6421 gaaggagtcg atgaagagag agcgcgccac ctcctcgtct ggacccggac ccatcggctc
     6481 gtacatctcc ggatagggct tcgggccgac ggtgtcgatc agcggcgtgg cgagcgcccg
     6541 aaacggcgcg acgacgcgct cgccctcatc tacctctccc gcgtagcaca gcaaagccgt
     6601 gatgacgagc tttccatgcg cctcggccgg caagaacggc ataggcggcg ccaccatgac
     6661 gttggcgatg gtcgagagct tctcaggggc ggcctccgcc tcggccacga acgaggcgat
     6721 gacctctggt gtggccggga gcaccagcat cccacccacg atcgtatcga cctcgtgcag
     6781 cttgtagcgg aagctgctca caaccccgaa gttgcctcct ccgccccgaa tcgcccagaa
     6841 gagatcggga tgcctctctg cgtccacata cacaagctct ccgtccgccg tgacgatctc
     6901 tgctgccagc acatcgtcta tggtgaggcc gtgcttgcga accaggtagc ccacccctcc
     6961 tcctagagtg atccctccta tccccacaga tcctgtgtcg ccgaagcccg tggcgaggcc
     7021 gtgtgctccc acagcggcgg tgtactcgcc tgccgtcaac cccgtctgtg cccaggcagt
     7081 gcgcccttcg gtgtcgagtt cgaggttctt catccccgag aggtcgagca cgatgcctcc
     7141 ttcggagatg ctgtgtccgg cgaggctgtg gccgccgctc tttatggcta gctccagccc
     7201 gctctcgcgt gcgagggaga cgacgcgccc gatgtcggag gcgtccttga cccggacgat
     7261 ggccgcaggg tgacggtcga aaccgccgta gaagacagtg cgcgcctcct tgtagatggc
     7321 gtcgtcgggc agtatgacct gcccccttac attggagtaa aacgtccgca tggccgcttc
     7381 atcgaggggc tccgcgtggg acagctctaa ggcatgttga cgattgtccg gcatgatcct
     7441 ggctcctatt cttgtttcgc tcctgtccaa catactgtgc ggcggggcga gagacatccc
     7501 ccgaaaggtg gagcgggagg atagagatct ctgcggtcaa gcagcgcagg aggtaagctc
     7561 tcttgagtag ccaggcagga caccgttatt cgcatccttt ctcagtcgtg agggttgcta
     7621 ctccgtacct cattagcacc tcgacggggc cgccttcccg gcagtatcga gaacgattcg
     7681 gcacctgatc actttcaatg tgcgagcgca ggtaacgacc gctgaacggt cctggatggt
     7741 cctggtccga gacccagttc gccgtcacga gtccccgaag ttgagagaac gctcgcgctt
     7801 ggattccgtg gtgtgctgac cgagggcagt gatccaagag tgcaacttca cagag
//
LOCUS       DATMVF010000016         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_186032, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000016 DATMVF010000000
VERSION     DATMVF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186032"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VN178_00510"
     CDS             <1..288
                     /locus_tag="VN178_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79471.1"
                     /translation="SLKTIMAGVNQVIMLSLSMVVIAGLAGAGGLGEPVVSGLSQLNT
                     GLSFVGGVGIVVIAIMLDRITRALARQDGGGAPKIFSRRKGSGTGATEEKA"
     gene            418..1329
                     /locus_tag="VN178_00515"
     CDS             418..1329
                     /locus_tag="VN178_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWS79472.1"
                     /translation="MNVSKRILALVSALLVAAVVLVGCGSGGGGGGSAQSENKTLTLG
                     NIGWTENVAVANLTKVILEEDLGYKEVELKTLDVGLLFEGVAGGDLHAFQDVWMPNHT
                     QLLAEVENDVEHLEPWYEGETSFGLAVPSYMTGVNSIADLNQSGAERIIGIEPGAIIS
                     TKIEENVIPDYNLTLEHEPSSTAAMLAEVERLYNNKEEFVFPPWCPHPMCSQFDFRYL
                     EDPKNSQGNLDQPAKISAIVNEDLPDDDPVAYAFINNLRLNEDELIAIENEIAAADDN
                     AVKGVQAWLEENRDVVKPAIDAAKQAQ"
     gene            1613..>2105
                     /locus_tag="VN178_00520"
     CDS             1613..>2105
                     /locus_tag="VN178_00520"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HWS79473.1"
                     /translation="MTATVVLGLAWGDEGKGRVCDALASDVDYVSRYSGGNNAGHTVR
                     VGAEEFKLHLVPSGIVREGVVCTIGNGVVVNPEVLVEEVEALEERGLGVRDRLKVDGR
                     AHLIMPYHIALDSHREIALGSARIGTTNRGIGPTYEDKVARYGIRVQDVFDEGILKTK
                     LKAA"
BASE COUNT          426 a          623 c          694 g          362 t
ORIGIN      
        1 tcgctcaaga cgatcatggc cggggtcaac caggtcataa tgctctcgct ctcgatggtg
       61 gttatcgccg ggctggccgg ggcgggggga ctcggggagc cggtcgtgtc gggcttgagc
      121 cagctgaaca ccgggctcag cttcgtcggc ggggtcggaa tcgtggttat cgccatcatg
      181 ttggaccgca tcacgcgcgc cctggcgagg caggatgggg gcggagctcc caagatcttt
      241 tcgcgccgta agggttccgg cacaggcgca accgaagaga aggcatgatg aggacctgca
      301 gatacgcggg tgtctggggc gtagcggggc aagaaaggga gagatctgat ccgtaagtgg
      361 cgtaccgacg taaatagatg cgcgtatcgg gaactgttgg ctgaaaaagg aaggtcgatg
      421 aacgtaagca agagaatact ggcgttggtg tctgcgcttc tcgtcgctgc cgtggtgttg
      481 gtgggttgtg ggagcggcgg aggcggaggg ggctctgccc agtccgagaa caagaccctg
      541 accctgggga acatcgggtg gacggagaac gtggccgtcg cgaacctgac gaaggtgatc
      601 ctggaagagg atctcggcta caaagaggta gagctgaaga ccctcgacgt cgggctgctg
      661 ttcgagggcg tcgcgggcgg cgacctccac gctttccagg acgtctggat gccgaaccac
      721 acgcagttgc tcgccgaggt cgagaacgac gtagagcacc tcgagccctg gtacgagggc
      781 gaaacctctt tcggtctggc ggtccccagc tatatgacgg gtgtcaacag catcgcggat
      841 ctgaatcagt ccggggccga gaggatcatc ggcatcgagc ctggcgccat tatctccacg
      901 aagatcgagg agaacgtcat acccgactac aacctcaccc tcgagcacga gccgtcgagc
      961 acggccgcga tgctggccga ggtggagcga ctctacaata acaaagagga gttcgtgttc
     1021 ccgccctggt gcccgcatcc gatgtgctcc cagttcgact tccgctacct cgaggacccg
     1081 aagaactccc agggcaacct cgaccagccc gcgaagatct ccgcgatagt caacgaagat
     1141 cttcccgatg atgacccggt tgcctacgcg tttattaaca acctcaggct caacgaggac
     1201 gagctcatcg caatagagaa cgagatcgcc gcggccgacg ataacgcggt caagggcgtc
     1261 caggcctggc tcgaagagaa ccgcgacgtg gtgaaacccg cgatcgacgc agccaagcaa
     1321 gcccagtaac cccgaatatc tcaccacgag ccgtggtacc ttcgcggccg gagcgcactt
     1381 cgtcgctccg gccgccaatc gtcgttcgac cagagcctgc cctgcgtaca taacagacga
     1441 tttttccccc ggcgatacaa aactttacat ctatgtgcta cgctgcgacc gtggcagtga
     1501 gcggcggccc cgatcggcgg acgatgagtt tcggtcctgg atcggggcta ctcctttttc
     1561 tcggacgtgg cgcccatttc gtaacggttc acgctagcgc ggaggtagag cattgacggc
     1621 cacggtcgtc ctcggactgg cctggggaga tgagggcaag ggtagggttt gcgacgctct
     1681 ggcgtccgac gtggactacg tctcgcgcta ctctggaggc aacaacgccg gccacaccgt
     1741 ccgtgtcggc gcggaggagt tcaagctcca cctcgtccca tcgggcatcg tgcgcgaagg
     1801 cgtagtctgc accatcggca acggggtcgt ggtcaacccc gaggtgctcg tcgaggaggt
     1861 cgaggccctc gaagagcgcg gcctcggggt gagggaccgt ctcaaggtgg acggtcgggc
     1921 gcacctgatc atgccctacc acatcgcgct cgactcgcac cgcgagatcg cgctggggag
     1981 cgccaggatc gggactacca accggggtat aggccccacc tacgaggaca aggtcgcccg
     2041 ctacggcatc cgggtccagg acgtcttcga cgagggcatc ctcaagacca agctcaaggc
     2101 ggccc
//
LOCUS       DATMVF010000017         7875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_461773, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000017 DATMVF010000000
VERSION     DATMVF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461773"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VN178_00525"
     CDS             <1..1349
                     /locus_tag="VN178_00525"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HWS79474.1"
                     /translation="GLPFQRKDGTLKTAFIGAGSVVFTKNLLTDTLSFPELHGTTIAL
                     HDIDPERLETAGMMARWTSEQLGADARVEEHLDRRTALEGADFVVNMVQIGMHQATLL
                     DFEIPKKYGLKQTIADSMGIGGIFRGLRTIPFVLDLAAEMRELCPRALLLNYTNPMSI
                     LMEALYTARPEIRSVGLCHSVPYTAREIASYIGVEHEELLYECAGINHIAWMTRLEVE
                     GKDAYPRLFAALEDPDVYAKDKVRFELMRRFGYFVTESSEHNAEYVPYFLKDDELIEL
                     YDVPVDEYIRRSEENLLQYAETRRRLLAGEDFSLGRSVEYGSLIMHSLVTGEARAVYG
                     NVENTGLVRNLPTGCCVEVPILVDDTGLRPCHVGDLPPQCAATCAPHTAVQNLTVRAA
                     LEGRRDHVHHAAMLDRHASSVLSLDEIAAMVDELIEAHGDAMPEGVRISGAGAGRV"
     gene            1390..2319
                     /locus_tag="VN178_00530"
     CDS             1390..2319
                     /locus_tag="VN178_00530"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWS79475.1"
                     /translation="MRNPTLPKLISQGFELDASDESFGLLRNSMDVIEDTGVLRERMA
                     EDGYLYLPGYLDRELVLEARASVTERLAAEGLTDPGYSADEAVAHPDSGLKFKPDLAH
                     DNEPLHHLLYGGRMMQFYERFLGGPVRHYDFTWMRAVAPGYGTQPHGDIVFMGRGTHD
                     LYTAWTPLGDIDYQLGGLMILEGSNRLENVREEYLSRDVDEYCKNVEGEEEHAKTNPE
                     MHGWRWDGSVSDDPVELREQLGGRWLTAEYRAGDLLTFSTYTIHASLDNRSQRIRLSS
                     DSRYQLASEPADERWIGEAPVGHGPAGKRGRIC"
     gene            2563..3888
                     /locus_tag="VN178_00535"
     CDS             2563..3888
                     /locus_tag="VN178_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS79476.1"
                     /translation="MSSESRVGRLSRRDFLKMGGGALAGAYAMGGLAGCGGGSVGGPT
                     ELTLWAWLPDFQTQIDMFEKAHKNIKVKLVNAGQGEDEYQKLRTALKAGSGAPDVVHM
                     EFQYIPTFRQIDGLADISEYGANDYKSDYAPWTWAQVSEGDAVYAMPWDSGPMALLYR
                     KDIFDEYGIEVPTTWDQFAQEAEKLHKADPETYLADFTPEDGGWLTGLMWQAGARPFE
                     VDGTKVSISLDDPAIMKMSEYWGDLVERGVVATDPGFTNDWYRSFAKGIYATWPTAAW
                     GPVFLEGVAGESKGKWRAAPMPQWEEGANASSNWGGSTLAVTTQSENAEAAAQLAIWL
                     THDPKPTELYTTKQFLFPVLNDLLDSPDFKNRKFDFYGGQAVNSVFVEASNNVNKGFE
                     WSPFQDYVYSQMEEQFGDAATGDTEFSQVPVNLQEKVTSYAKQQGFKVS"
     gene            3936..4886
                     /locus_tag="VN178_00540"
     CDS             3936..4886
                     /locus_tag="VN178_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS79477.1"
                     /translation="MAVEQGNEAGTTAPEAKPPGRAARNRRLRRVVIPYLFLLPFLVL
                     FVVFLVLPLAYALGISVFENRLVGGMVFVGADNYLRAIGDPAFLGGVFRMLKFGVFQV
                     PIMLGLALLLALVLDSGVVWFRSAFRLGFFLPYAVPSVVATLIWGYLYGQDFGPIAQL
                     AERLGLPAPNFLSDSLVLGSLANIVTWQFTGYNMIILYAALQAVPGELRDAGAVDGAR
                     GWQVAWYIKIPLILPALFLTAVFSIIGTFQLFNEARILQPQAPGAIGPAFTPNLYAYS
                     LAFINQQYEYSAAVSFALGAVVVVCSYVFMLVTNRREVGD"
     gene            4886..5767
                     /locus_tag="VN178_00545"
     CDS             4886..5767
                     /locus_tag="VN178_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS79478.1"
                     /translation="MAPITGRRSGPRESQFTRVLTTVLLTIFLVYSLLPVFYLIVSST
                     KDNADLFSSFGLWFASFSLTENLRDTFARDGGVFWNWLWNTAYYSVCSAVGAAFISTI
                     AGYAFAKYRFPGRVPVFAVILGSIMVPQTALVIPTYLLLSKIGLIDTPLAVILPSLVS
                     PFGVYLMFVYAEQSVPDDLLNAARVDGAGELRIFWSVALRVLMPGFVTVLLLSFVATW
                     NNYFLPLVVLSTPELYPLSVGLVSWNDLATAGGGAQPLFPLVITGALISVIPIIVSFL
                     FLQRYWQGGLTFGSLKD"
     gene            5833..7836
                     /locus_tag="VN178_00550"
     CDS             5833..7836
                     /locus_tag="VN178_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWS79479.1"
                     /translation="MITPRVPRMIYGGDYNPEQWPEEVWDEDVRLMREAGVNLVSLGI
                     FSWAKLEPRPGEYDFGWLDRIISLLHEGGVMVNLATATASPPPWLAKLHPESLPVTQS
                     GVVLHPGARQHYCPSSAAYKERASELVRRIADRYKDHPALAMWHVNNEYGCHVAECYC
                     DASAVHFREWLRARYGDLDALNEAWGTAFWSQRYGEWGEILPPRSAPTFANPTQQLDF
                     RRFSSDALLELYEMEKEILRQATPDIPITTNFMGFFKPVDYWKWAAREDLVSDDSYPD
                     PSDPEAHIRAAMSRDLMRSLGDGAAWVLMEQTTVRVNWRKRNVPKRPNEMRLWSYGAV
                     ARGAEGIMFFQWRQSRAGAEKYHSAMVPHVPTEDSRSWREVSRLGSELGKLDELLGAR
                     GEAETAILLDWESWWALELDSKPSTAVRMLEGLYSFYKPLYWANVPVDFAHPGSDLSS
                     YKLVIAPNLYLVTDDSAENIRRFVADGGTLIMSFFSGIVDARDHIRLGGYPAPFKELL
                     GLHIEDFVPMATGETNRLDTLDGASCDMWADLIHLEGAESLASHTEDFYAGTPAVTRN
                     LVGEGVAYYLGTRPEERYTKALLQKVYEEAGVRPTAEVPPGVDAVRRKTEAASFLFLL
                     NHNEEAVEVQLPNAGRDLLIGAEHDSQLILDPLGVAILQEITP"
BASE COUNT         1491 a         2489 c         2418 g         1477 t
ORIGIN      
        1 gcgggttgcc cttccagaga aaggacggaa ccttgaagac agcctttatc ggcgcaggga
       61 gtgtcgtctt taccaagaac ctcctgacgg acaccctgag cttccccgag ctgcacggaa
      121 ccactatcgc cctgcacgac atagatcccg agcgtctgga gaccgcgggc atgatggccc
      181 gctggacttc agagcagctc ggcgccgatg cgagagtcga agaacatctc gaccggcgaa
      241 cggcgctcga aggggccgac ttcgtagtga acatggtcca gatcgggatg caccaggcca
      301 cccttctcga cttcgagatc cccaaaaagt acggtctcaa gcagacgatc gccgactcga
      361 tgggtatcgg cggcatcttc cgtggcctgc gcacgatacc cttcgtgctc gacctagctg
      421 ccgagatgcg agagctctgc ccgagggcat tgctactgaa ctacacgaac cctatgagca
      481 tcttgatgga ggcgctctat acagcccgcc ccgaaattag aagcgtcggg ctctgccaca
      541 gcgtccctta caccgcgcgc gagatagcct catacattgg cgtcgagcat gaggagcttc
      601 tctacgagtg cgccggcatc aaccacatcg cctggatgac acgcctcgaa gtcgaaggca
      661 aggacgccta cccgcggctc ttcgcggcct tggaagaccc tgacgtgtac gccaaggaca
      721 aggtgcgatt cgagcttatg cgtcgcttcg gctacttcgt caccgagtcg agcgagcaca
      781 acgccgagta cgtaccgtac ttcctcaagg acgatgagct gatcgagctc tacgacgtac
      841 ccgtggacga gtacatcagg cgcagcgagg agaaccttct ccagtacgcc gaaacccgcc
      901 gcagactgct cgccggagag gacttctcgc tggggcgcag cgtggagtat ggttcgctca
      961 tcatgcactc gctcgtcaca ggcgaggcgc gcgccgtcta cggcaacgtc gagaacaccg
     1021 gcctcgttag gaacctccct acaggttgct gtgtcgaggt gccgatcctg gtcgacgaca
     1081 ccggtctgcg tccctgccac gtgggagacc tccctccgca gtgcgccgcc acctgcgccc
     1141 cgcacaccgc cgtccagaac ctcacggtga gggccgctct ggagggaagg cgggaccacg
     1201 tccatcacgc tgccatgctc gaccgccacg cctcgagcgt cctctccctc gacgagatcg
     1261 cagcgatggt ggacgagctc atagaagccc acggcgacgc catgcccgag ggcgtcagga
     1321 tctcaggcgc gggagcagga cgcgtctagg aatgtacaaa gacaccatga cgaacccgag
     1381 gtggtagcag tgaggaatcc cacactcccc aagttgatct cgcaaggttt cgagctcgac
     1441 gcgtctgacg agagcttcgg cctgctccgg aactcgatgg acgtcatcga agacacagga
     1501 gttctcagag agaggatggc cgaggacggg tacctctatc tgcctggcta tctggaccgc
     1561 gagctcgtcc tcgaagccag ggcgagtgtt accgaaaggc tcgccgccga agggctcacc
     1621 gacccgggct actctgccga cgaggccgtt gcgcatcccg actcggggct caagttcaag
     1681 ccggatctcg cccacgacaa cgagccgctg caccacctgc tgtacggcgg gcgcatgatg
     1741 caattctacg agcggttcct cggcggtccc gtgcgccact acgacttcac ctggatgagg
     1801 gcggtggcgc cgggctatgg tacgcaacca catggggaca tagtgttcat gggacgtggt
     1861 acgcacgatc tgtataccgc ctggacccca ctgggagaca tcgactacca gctcggaggc
     1921 ctgatgatcc tggagggctc gaaccgtctt gagaacgtgc gtgaggaata cctgagtcgg
     1981 gatgtagacg agtactgcaa gaacgtggag ggtgaggagg agcacgccaa gacgaacccg
     2041 gaaatgcacg gttggcggtg ggacggcagc gtctcagacg accccgtcga gctgcgcgag
     2101 caactcgggg ggagatggct cacagcggag tacagggccg gagacctgct gacgttcagc
     2161 acctacacca tccacgcgag cctggataat cgctcccagc gcattcgcct ctcttcagac
     2221 tcgcgttacc agctcgcatc agagcctgcc gacgaacgtt ggatagggga ggcgcccgta
     2281 ggccacggtc ccgccggcaa acgcggacgt atctgctgaa atgggaccgg gttcaggctg
     2341 cggatactca cagaggcttc cctcacacgt cattcccgcg caagcgggaa tccacatcgc
     2401 ttgacacgcg atgagattgg tccaacaatg cgatgagaag taaccagttt gtggccgtat
     2461 tcgtgcaggg gacatgcgtt tggacggcct tggggtgaga ttccacagac gcgggttgct
     2521 cgatgtgcca ggggatgcga gacgacttcg gaaggaggag acgtgagtag cgagtccagg
     2581 gtggggcgtc tgtcgcggag ggacttcctc aagatgggtg gaggcgcgct cgccggcgca
     2641 tacgccatgg gagggctcgc tggttgtggg gggggatcgg taggcggccc gaccgagctt
     2701 accttgtggg cgtggttgcc tgatttccag acgcagatag acatgttcga gaaggcccac
     2761 aagaacatca aggtcaagct ggtcaacgcc ggacagggtg aggacgaata ccagaagctg
     2821 cgtaccgctc tcaaggccgg ttcaggggcc cccgacgtgg tgcacatgga gttccagtac
     2881 atccctactt tcaggcagat cgacggtctt gccgacatct ccgagtacgg cgcgaacgat
     2941 tacaagagcg actacgctcc ctggacgtgg gcgcaggtct ccgaaggcga tgcggtctac
     3001 gcgatgccgt gggacagtgg tcccatggcc ctgctgtatc gcaaggatat cttcgacgaa
     3061 tacggcatcg aggtccctac gacctgggac cagttcgccc aggaggcgga gaaactccac
     3121 aaagccgacc cggagaccta cctggcagac ttcacgcccg aggacggggg atggctcacc
     3181 ggtctgatgt ggcaggcagg ggcgcgtccc ttcgaggtcg atgggacgaa ggtttctatc
     3241 agcttggacg acccagcgat catgaagatg tccgaatact ggggcgatct ggtcgaacgt
     3301 ggggtcgtcg cgacagaccc gggcttcact aacgactggt acaggtcgtt cgccaaaggc
     3361 atctacgcga cctggccgac cgctgcgtgg ggtcccgtgt tcctcgaagg cgtcgctgga
     3421 gagtccaagg gtaaatggcg ggccgcgccg atgccgcagt gggaggaagg cgccaatgcg
     3481 tcatccaact ggggcggttc gacgctcgcc gtcaccacgc agagcgagaa cgccgaggcg
     3541 gcggcccaac tcgctatttg gctcacccac gaccccaagc cgaccgagct gtataccacc
     3601 aagcagttcc tcttccccgt gctcaacgat ctcttggaca gcccggactt caagaatcgc
     3661 aagttcgact tctacggcgg ccaggccgtc aactccgtct ttgtcgaggc ctccaacaac
     3721 gtgaacaagg gcttcgagtg gagcccgttc caggactacg tatacagcca gatggaagag
     3781 cagttcgggg atgcggcgac cggagacacc gagttctccc aggtcccggt gaatctccag
     3841 gagaaggtca caagctacgc caagcagcag ggcttcaagg tcagctagcg tgaggcccgg
     3901 cggctgaggt ccgttcggga gcggggaggt atgcgttggc ggtagagcag ggaaacgagg
     3961 ccgggacgac cgctcccgaa gccaagccgc cgggaagggc ggcacgcaac agacggttgc
     4021 ggcgggtagt cattccttac cttttcctcc tgcctttcct cgttctgttc gtcgtcttcc
     4081 tcgtgctccc gcttgcgtac gcgctaggga tcagcgtctt cgagaaccgt ctcgtcgggg
     4141 gcatggtctt cgtgggcgcg gataactatc tgagggcaat cggggacccc gcctttctgg
     4201 gcggggtctt caggatgctg aaattcgggg tgttccaggt gccgatcatg ctcggtctcg
     4261 ccctcctgct cgccctggtc ctcgacagcg gcgtggtatg gttccgctcc gctttccggc
     4321 tcgggttctt cctgccctac gccgtgccga gcgtggtggc cacccttatc tggggttacc
     4381 tctacggcca ggacttcgga cccatcgcgc agctggccga aaggctgggt ctcccagccc
     4441 ccaacttcct ctccgactcg ctcgtacttg gctccctggc caacattgtg acctggcagt
     4501 tcaccggata caacatgatc atcctctacg ccgccttgca ggccgtgccc ggagagctca
     4561 gggacgctgg ggccgtggac ggggccaggg gttggcaggt ggcatggtac ataaagattc
     4621 ccctgattct gccagccctg ttcctgacgg cggtcttctc gatcatcggc acgttccagc
     4681 tcttcaacga ggcccggatc ctgcagccgc aggcacctgg agccatagga ccggcgttca
     4741 cgcccaacct ctacgcctac tccctggcgt tcatcaacca gcagtatgag tattcggccg
     4801 cagtctcctt cgcgctcggt gcagtggtgg tcgtatgctc ctatgtcttc atgctggtga
     4861 ccaatcgcag ggaggtgggt gactgatggc cccgatcacg gggaggcgca gtggtccgag
     4921 agagagccag ttcacgcgtg tgctcaccac ggtcctactt accatcttcc tggtctactc
     4981 gctgctccct gtcttctacc tgatcgtctc gtccacaaag gacaacgcag acctcttctc
     5041 ttcgttcggg ctgtggttcg cttccttcag cctgaccgag aacctgcgcg acaccttcgc
     5101 ccgtgacggc ggcgtgttct ggaactggct ctggaacacc gcgtactatt ccgtctgcag
     5161 cgccgttggc gctgcgttca tctcgaccat agcgggttac gccttcgcca agtaccgctt
     5221 tccagggcgc gtaccggtct tcgcggtgat cctgggttcc attatggttc ctcagaccgc
     5281 gctggtgatc ccgacgtatc tgctccttag caagatcggg ctcatagata ctccgttggc
     5341 agtgatcctg ccctctctgg tctcgccttt cggcgtctac cttatgttcg tctacgccga
     5401 gcagtcggtc cccgacgacc tgctgaatgc ggcccgcgtg gacggggccg gcgagctcag
     5461 gattttctgg agcgtcgccc tgcgtgtcct gatgcccggc ttcgtcaccg tcctcctcct
     5521 gtccttcgtt gcgacctgga acaactactt ccttcctctc gtggtcctga gcactcccga
     5581 gctctacccg ctgagcgtcg gcctggtctc gtggaacgat ctagcgaccg cgggcggcgg
     5641 ggcgcagccg ctctttcctc tcgtcataac aggggccctt atctccgtca tacccataat
     5701 cgtgtctttc ttgttcctgc aacgttactg gcagggcggc ctgaccttcg gcagcctgaa
     5761 ggactgagcg tcattcgtcg ccgggaatca cggatcaccc ccggcgacgg tccgacaaca
     5821 ggaggtaagc ctatgatcac ccctagggtc ccgcgcatga tctacggcgg cgactacaac
     5881 ccggagcagt ggcccgagga ggtctgggat gaagatgtgc gcctgatgcg cgaggccggc
     5941 gtgaacctgg tctccctggg gatcttctcc tgggcgaagc tggaaccccg tccaggcgag
     6001 tacgacttcg gctggctcga ccgcatcatc tccctcctgc acgaaggcgg cgtgatggtg
     6061 aacctcgcca ccgccacggc ctcgccacca ccctggcttg ccaagctcca ccccgaaagc
     6121 ctgcccgtta cgcagagcgg cgtcgtcctc catcctggcg cccgccagca ctactgcccg
     6181 agcagcgctg cctacaagga gagggcttcc gaactcgtcc ggcgcatagc ggaccgctac
     6241 aaggaccacc ccgcgcttgc gatgtggcat gtgaacaacg agtacggatg ccacgtggcg
     6301 gagtgctact gcgacgcctc ggccgtccac ttcagggagt ggttgagggc gcgctacggc
     6361 gacctggatg ccctgaacga ggcgtggggg acggcgttct ggagccagcg ctacggggag
     6421 tggggtgaga tcctacctcc tcgcagcgcc cccaccttcg cgaaccctac ccagcaactg
     6481 gacttccgcc gcttctcctc tgacgctctg ctcgagctct acgagatgga gaaggagatc
     6541 ctgcgacaag cgacaccgga tatccccata actaccaact tcatgggctt cttcaagccc
     6601 gtggactact ggaagtgggc ggcgcgcgag gatctcgtct cggacgactc ctaccccgac
     6661 ccctctgatc ccgaggccca catacgagcc gccatgagcc gcgacctgat gcgctccttg
     6721 ggagatgggg ccgcgtgggt cctcatggaa cagacgacgg tgcgcgtgaa ctggcgcaag
     6781 cgcaacgtac cgaaacgtcc aaacgagatg cgcctctgga gctacggggc cgtcgcccgc
     6841 ggcgcggagg gcataatgtt cttccagtgg cgccagtcca gggcgggagc cgagaaatac
     6901 cacagcgcta tggttccgca cgttcctacg gaagactccc ggagctggcg cgaggtcagt
     6961 cgactcggtt cggagctcgg aaagctggac gaactcctcg gggcgcgcgg ggaggctgag
     7021 accgcgatcc tgctcgactg ggagagctgg tgggccctcg agctcgactc caaaccctcc
     7081 accgccgtca gaatgctcga gggcctctac tccttctaca agccgctcta ctgggcgaac
     7141 gttcccgtag acttcgccca tcccggctcc gacctctcgt cctacaagct tgtgatcgct
     7201 cctaacctct acctcgtcac cgacgactcg gcggagaaca tccggcggtt cgtggctgat
     7261 ggtggaacac taattatgtc tttcttctcg gggattgtgg acgcgcggga tcacatccgc
     7321 ctcggcggtt acccggctcc gttcaaggag ctgctgggtc tccatatcga ggacttcgtc
     7381 ccgatggcaa caggagaaac caatcgtctc gacaccctcg acggcgcatc ttgtgatatg
     7441 tgggcagatc tcatccacct cgaaggtgcg gaatccctgg cctctcacac ggaagatttc
     7501 tatgcaggca cgccggccgt aacgcgcaac ctcgtcggtg agggcgtagc ctactacctc
     7561 ggcaccaggc cggaagaacg ctacacgaag gcgctcctcc agaaggtgta cgaggaggcc
     7621 ggtgtgagac caacggccga agtaccaccc ggtgtcgacg ctgtgcgccg caagaccgaa
     7681 gccgcatcct tcctcttctt gctcaaccat aacgaggaag ccgtagaggt tcagctaccg
     7741 aacgctggcc gggatctcct cataggagcg gagcacgatt cacagctgat cctggaccct
     7801 ctcggagtcg cgatactgca agagataacg ccgtgaggtt gccgcgtaag ggagagttgt
     7861 agatgttctg tgggc
//
LOCUS       DATMVF010000018        12097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_1641, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000018 DATMVF010000000
VERSION     DATMVF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12097
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VN178_00555"
     CDS             complement(<1..736)
                     /locus_tag="VN178_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS79480.1"
                     /translation="MTPAISLRGIGKKYRISPSRSSRLGEILSFGKAKRSHEFWALQD
                     INLDVEPGTTLGVVGRNGAGKSTLLSIISGVLRPTTGEVEVRGRLSAIFGIGTGFNPL
                     FTGRENAMLSGLILGIEHDEMVERFDDIEAFADIGEFMDQPIKTYSSGMRSRLGFAVA
                     INVEPDVLVIDEALSAGDSAFKKKAIQRMYDLKDSGSTVLFVSHSMGMVKRFCTEAIL
                     LHKGRLITTGSPEEVVDHYEEMLSKSQ"
     gene            complement(733..1578)
                     /locus_tag="VN178_00560"
     CDS             complement(733..1578)
                     /locus_tag="VN178_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79481.1"
                     /translation="MATYTYNGKRSFAGEFFDSLASFYRKRWLLRYFVRRQVTRSYKR
                     SYLGLAWAILGPLIWVFFLTLIFSEVVGLRFRVVEEDPTLNFGLFLYCGLLPFMAFSE
                     ALTKGLNSIRNNAGLVQKVVFPLELLPFTNAVASLVDKLFGIGALVFIVFVLQHRLHW
                     QVLLLPLIIVLQLLFTLGLTYIMAVVGTYVPDMGEVLRPIIRGTFFITPILWPPETLP
                     ENLQFINDYNPLAYLVGAYRDMILEGTLPGGLATLYFALFSGGLFLAGFALFVRVKPG
                     FADHL"
     gene            complement(1694..2941)
                     /locus_tag="VN178_00565"
     CDS             complement(1694..2941)
                     /locus_tag="VN178_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWS79482.1"
                     /translation="MRVKVMEAGDGRLQGYTFAAALLWAVLLLILAPGSKGEPATRPG
                     VDNDPEGAPYVAGELLVAYEPGTSEEAEETVVRRSGARTLKNLPGEVRLVSFPAIRRE
                     ASEVVRERALARALGHLAHRAGVVAADYNYLREPYFMPDDPRLANQWGLASTKFSGAW
                     NDAKGSGAKVAIVDSGVYSEHPDIGRITAQRDFVEDDAVADDDYGHGTHVTGIAGALT
                     DNGKGVAGGCFGCGLIIGKVMGLGGFTTDSRIVEGIDWSVNQGADVVNLSLGGPADSS
                     VLRGAVNRAHGEGAVLVAAAGNDGADGPRYPAAYSKVIAVGAISADDRLASFSGRGRW
                     VDLAAPGTDIISTSESGGYDTQSGTSMAAPFVSGLAGLLASQGMSAGSIRQEMQATAE
                     DLGPAGQDPRYGHGRIDAANAVR"
     gene            3188..4123
                     /locus_tag="VN178_00570"
     CDS             3188..4123
                     /locus_tag="VN178_00570"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79483.1"
                     /translation="MTPRFKRAWRRAVGKGESLRPRARRSSRGVRPENIVWIFGTGRS
                     GSTWLGSMMEGLEGCAMWNEPLVGNLFGNFHYFRVGGRKTGGQYILGEPFKETWLGPM
                     RDLVLGGAAARFPEVSGYLIIKEPNGSIGAPLLMEALPESRMIFLIRDPRDVVASSMD
                     ARSEGSWLSERREAQRSTSKQIRNPNVYVRMRANSYVQQIEKTWQAYRAHGGRKVMVR
                     YEDLRADTLQTMKRIYSSLEIPVRATDLARSVEKHSWEKIPSEQKGEGKKRRKAKPGG
                     WREDLTARQVEIVEDITAPLLNEFYPHTATTRGAL"
     gene            4120..5037
                     /locus_tag="VN178_00575"
     CDS             4120..5037
                     /locus_tag="VN178_00575"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79484.1"
                     /translation="MIRDAWRKVRGRGMLEENGGMRPENLVWVFGTARTGSSWLSAIM
                     GEVGGYCRWHEPLVGHLFGNLYYERAGHRSEDEHFILGARHRELWLGTIRRFVLDSAA
                     ARFPGIAAKGGGYLIIKEPQGSMGAPLLMEALPESRMILLVRDPRDVVASNLDAHKGG
                     TWTADLMRKGGKEKPPSLAESRPDAFVKGQARRYVRDVGNAKKAYDAHGGLKVLVRYE
                     DLRTDTLQTMKRIYSELEMPVEEADLARSVEKHSWEKIPSEQKGEGKFHRKAKPGGWR
                     EDLTARQAEIVEGVAGSLLRELYPVRSTA"
     gene            5048..6022
                     /locus_tag="VN178_00580"
     CDS             5048..6022
                     /locus_tag="VN178_00580"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79485.1"
                     /translation="MPVFRHPKVSGAARRIRAVLGLEAGVTRGALVERTGTDGRSAGG
                     VAPENMIWIFGTGRTGSTWLAAMMEEPEGHDVWFEPRVGTIFDPQRFQRHGGKHFILA
                     SQYKDVWLRSIRNFILDGANARFPELDGGYLAVKEPGGSVGAGLIMEALPESGMVLLI
                     RDPRDVVASWLDATRKGGWQTRRRGEGGRRAESLAETNPNAFVRRHADAYLQHVGSAR
                     RAYEAHEGRKVVVRYEDLRADTLGTIKRMYGELNVPVEEAKLVKAVEKHAWENIPEEE
                     KGRGKFYRKATPEAWREDLTRRQVKTVERITAPLLEEFYPTGPAEQRG"
     gene            6049..7143
                     /locus_tag="VN178_00585"
     CDS             6049..7143
                     /locus_tag="VN178_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79486.1"
                     /translation="MSTSRYRDALRSLREGSARLGPAVDRLRERLGRQSPNGTAFRDR
                     EIPVFFVVGLAKSGTTWLMKTLDAHPEVLCKGEGRFFGEEYRREVLAQTQTRQQPSSL
                     YNALLGSEYLRLWIERSVWSRDGDPDEHVRDLVRLATDYFLTQELSKSGKKVVGDKTP
                     LLGPKFVEEIYEIYPEARVIHIIRDGRDQAVSFIHQQGNRAKRGRTHRLSPEELARSE
                     AYRRSSRDLAQRGEGMFAEKTLRKAAQNWALRVGRAIEDGPALFGDRYTEVRYEDLLE
                     RPNEEVGRLLGFLGVNRDEALVEHCVSSASFQKLSRGRERGQEDPSSFYRKGVAGDWK
                     NHFSEEDRRVFKEEAGELLIRLGYEKNGGW"
     gene            7146..8135
                     /locus_tag="VN178_00590"
     CDS             7146..8135
                     /locus_tag="VN178_00590"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79487.1"
                     /translation="MEGRIRKAKAGLPGVLRKGRRWLAGKYREVPLLAASLRRRFDGG
                     PASGVRPENIVWIFGSGRSGSTWLRSMMGDPSRHLVWEEPMVGQLFGNFHRRAEKANL
                     IRPDFVMADATRRGWIHSIRNFVLDGARYARPRLGPDHYLIIKEPNGSVGAPLLMEAL
                     PESRMVLLIRDPRDVVSSTLDGARKGNWLYERKDKGGWKRDSLPDEDPDAFTRRWANV
                     YMQHAGNAKMAYDAHRGPKVMVRYEDLRADTLGTMRRIYSTLGVPVDEHELARAVEKH
                     SWENIPEKKKGGGKFYRKATPGGWQEDLTPKQIKQVERITAPLLEEYYSGRTL"
     gene            8138..9109
                     /locus_tag="VN178_00595"
     CDS             8138..9109
                     /locus_tag="VN178_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWS79488.1"
                     /translation="MAEKVSVVIPTFNAGPAFGELLRRLDAQEGDFEREVIVVDSGST
                     DGTAQLAARQGAILHNVSKASFDHGATRDLGISLSSGEYVALTVQDAVPLDARWLVTM
                     VENLQSDGRIAAVYGRHVPRPDAGAMTRALVGNLAVANTERREQEIPDTAQYLSLPPS
                     QRRRIAAFDNVSSCLRRSVWEEFPFGEADFAEDLRWGKKVVEAGYTIVYEPRSVVVHS
                     HERGALYDLRRHYVDQLVLDELFGARLVPNLPRLILGIPYATHHVYGLLRREEPAPER
                     RPKLLLDAATFALASQLGAYLGCKLPSLSQPTPKLHARLHRFLGKGV"
     gene            9335..10489
                     /locus_tag="VN178_00600"
     CDS             9335..10489
                     /locus_tag="VN178_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79489.1"
                     /translation="MLTAIIYAMNLERVVERLRDYRLKNERFDQLVGAVLSNEKAMRA
                     ASATVAAARSVSRSGQTVAAGDYDTVFFVAGEMRSGTSWLRHSLSDHPEIACGQEGSF
                     FGRGYDHEEIPVYAGPVSSLTRALAVSRELKTWHALPWNLWSDGYDEDLRNLSRLCVD
                     YFLCKEVARTGRRIVGDKSPQHTENLDEIYEIYPDARIIHIIRDGRDVAVSAMHHWWR
                     LARDREGGVFELSPEELRIRDDYAEDRERFLSEGRSIFTRERLAQLASRWEYRTGKGS
                     HDGRTLYGERYLEIRYEDLLVDTNSVFRKILEFLGAGSEDRVIERCLRSSSFERASSR
                     RQGEEDSASFFRRGVAGDWRNVFTERDRELYKQLAGERLVEFGYEKSYDW"
     gene            10493..11833
                     /locus_tag="VN178_00605"
     CDS             10493..11833
                     /locus_tag="VN178_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWS79490.1"
                     /translation="MRILFAAHQFFPEHYAGVETVTLSLAQEFKARGHEPYVLAPKRS
                     IPGNDIEPGEVEDYEFGGIPVRRVGRPKEGVSRPYSLDYENGVMALRTREYLRKISPD
                     IVHAEHFQGLSASVIPAIKEFGVPLVYTATDFWTFCPVVDLRRHDGVMCRGPEISHCT
                     RCIASRYPGTRMKQAVDLTPPAAIKLAGALSETRLSGLSHSLRQVRALKERPERIREN
                     MGLVDHVIVYTHLMRELLLANGIGAGKIEVSHYGIDTSGIRREDRPFSPPLRVGFVGT
                     LAPHKGCDVLIRAFRDLPPEVDATLDIYGNLERFKPFVEELRALAGGDGRIDFAGPFA
                     REEVGDVLSGMDVLVVPSRWYENGPGVIFEAFAAGIPVVATNLGGMSEFVRHERDGLL
                     FGLEDHGGLASQLRRLVEEPGLLERLRAGIEPVKTVQENVDELEVLYGGLLARV"
     gene            11839..>12097
                     /locus_tag="VN178_00610"
     CDS             11839..>12097
                     /locus_tag="VN178_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HWS79491.1"
                     /translation="MPKFLVSGSGGQLGLELAALLPRRGHEVIALSKGELDVADFGAV
                     RLALEEHAPDIVVNAAAYTNVDGCESEADLAYRVNALGPRNL"
BASE COUNT         2371 a         3757 c         4003 g         1966 t
ORIGIN      
        1 cctgagactt cgaaagcatc tcctcgtagt ggtcgaccac ttcctcgggg ctgccggtgg
       61 ttatgagcct tcccttgtgg agcagaatgg cctccgtgca gaatcgcttt accataccca
      121 tgctatggga gacgaaaagg accgtggaac cggagtcctt gaggtcgtac atcctctgga
      181 tcgccttctt tttgaacgcc gagtcgcccg cggagagggc ctcgtctatg acgaggacat
      241 cgggctcgac gttgatcgcg acggcgaaac ccagacggct gcgcatcccg ctcgagtagg
      301 ttttgatcgg ctggtccatg aactccccga tgtccgcgaa agcctcgatg tcgtcgaagc
      361 gctccaccat ctcatcgtgc tctatgccga ggatcaagcc gcttagcatg gcgttctcgc
      421 gtcccgtaaa gagcgggttg aaccccgtcc cgatgccgaa gatcgccgac agccggcccc
      481 ggacctcgac ctctcccgtc gtcggccgca gaacgcccga gattatgctc aaaagcgtac
      541 tcttcccggc cccgttacgg ccaacgacgc ccagggtggt gcccggttcc acatccaaat
      601 tgatgtcctg cagcgcccag aactcgtgac tccgtttagc cttgccgaag ctcaggatct
      661 cccccagcct cgaggaccgg ctcggggaga tcctgtactt cttgcctatt cccctgaggg
      721 agatggccgg cgtcacaggt ggtccgcaaa ccccggcttc accctgacga acagcgcgaa
      781 gccggccagg aacaggcccc cggagaacag ggcgaaatag agcgtcgcca gcccgccagg
      841 gagcgtaccc tcgaggatca tatccctgta tgcgcccacc aggtaggcca gcgggttgta
      901 gtcgtttatg aattgcaggt tctccggcaa cgtctcgggc ggccagagga tcggtgtgat
      961 gaagaaggtt cccctgatta tgggtcttag gacctcgccc atgtcgggca catacgttcc
     1021 gactaccgcc atgatgtagg tcagacccag ggtaaacaac aactgcagca cgatgatcag
     1081 gggcagcagc aagacctgcc agtgcagcct gtgttgcagg acgaagacga tgaatacgag
     1141 cgctccgatg ccgaagagct tgtcgaccag cgatgctacc gcgttggtaa aggggagcaa
     1201 ctccagcggg aagaccacct tctgcaccaa tcccgcgttg ttcctgatgc tgttgagtcc
     1261 cttggtcagc gcttcggaga aggccatgaa agggagcaga ccgcagtaca aaaacagccc
     1321 gaagttgagc gttggatcct cctctaccac cctgaacctc aggccgacca cctcagagaa
     1381 gatcaacgtc aggaagaaga cccagatcag gggtcccagt atcgcccacg cgagacccag
     1441 ataggacctc ttgtagctac gggtcacctg gcggcggaca aagtacctga gcaaccaacg
     1501 tttgcggtag aacgacgcca gggagtcgaa gaactccccg gcgaagcttc tcttaccgtt
     1561 atacgtgtac gtggccaatt acgtccctcg catcgtaggc agcgccgcca gtagtgtaag
     1621 ccatgtgccc gagaatgtgg gcagcggcaa gtttacccaa gcaagcatgt gcttgccagg
     1681 ttgcgtcgct agactatctc acggcgttgg cggcgtcgat cctgccatgg ccgtagcggg
     1741 gatcctgccc cgcgggaccg agatcctcgg cggtggcctg catttcctga cggatcgagc
     1801 ccgcggacat gccctgggag gcgagcagcc cggcgaggcc ggacacgaac ggcgccgcca
     1861 tgctcgtccc gctctgcgtg tcgtagccgc ccgactcgct ggtagagatt atgtccgtcc
     1921 ccggcgcggc cagatccacc catctgccgc ggccggaaaa ggaggcgagc ctgtcatccg
     1981 cgctgatggc gcccacggcg atcaccttgg aatacgccgc cgggtaccgc gggccgtccg
     2041 ccccatcatt gccggccgcg gccaccaaga cggcaccctc gccatgtgcg cggttgaccg
     2101 cccccctcag cacactggag tctgccggcc cgccgagcga gaggttcacg acgtcggcac
     2161 cctggttcac gctccagtcg ataccctcca ctatccgcga atccgtggtg aaccctccca
     2221 agcccataac cttgcctatg ataagcccgc acccgaagca tcccccggcc acgcccttgc
     2281 cgttgtccgt gagcgcgccc gctatgcccg ttacgtgggt gccgtgcccg tagtcgtcgt
     2341 cggccaccgc atcgtcctcg acgaaatctc tctgggctgt gatcctgcct atatctggat
     2401 gctctgagta gacgcccgag tccacgatgg cgaccttcgc cccgctgccc ttcgcgtcgt
     2461 tccaggcgcc actgaacttc gtgctggcga gcccccactg gttggcgagc ctcgggtcgt
     2521 caggcatgaa gtaaggctcg cgcaagtagt tgtagtcagc ggccacgacc cccgctctgt
     2581 gcgcgagatg ccccagcgcc cgtgcgaggg cccgctcccg caccacctcg gatgcctcgc
     2641 gcctgatcgc cgggaaggag acgagcctca cctcccctgg aagattcttc agggtccgcg
     2701 ctcccgaccg cctgacgacg gtctcttcgg cctcctcgga ggttccgggc tcgtaggcga
     2761 ctaggagctc cccagcgacg tacggcgcgc cttcggggtc gttgtccacc ccgggccgcg
     2821 tggcaggttc ccccttcgac cccggagcga ggatcagtag gaggaccgcc catagtagag
     2881 cagcggcgaa agtgtaccct tgaagacgac cgtcgccggc ttccatgact ttgacccgca
     2941 aatatccccc tacaagaaga ccacaccgtt gcgcaaggcc acattatcta acaaaagcgc
     3001 agaattgtta aggggttttg tgatccattg cgcgaaaaga tgctcggggt tcgcgacggt
     3061 cggcgtcact aaacggggcg ctgtaagagt ataatagccc atcgcatgta gataagtctt
     3121 atgggagcga atcgttgcaa tcaccttgtg gccgacaagc cctcgggagg gcttgggagg
     3181 aacaaccttg acgccgaggt tcaagcgggc ctggcggcgc gcggtaggaa agggcgagtc
     3241 cctccgcccg cgggcccgac gatcgtcgcg cggcgtaagg ccggagaata tcgtctggat
     3301 cttcggcacg ggccgcagcg gcagcacgtg gctcggctcc atgatggagg gcctcgaagg
     3361 atgcgccatg tggaacgagc cgctcgtagg aaacctcttc ggcaacttcc actacttcag
     3421 ggtggggggg cgcaagacgg gcgggcagta catcctggga gagcccttca aagagacctg
     3481 gctcgggccc atgagggatc tggtcctcgg cggggcggcc gcccgctttc ccgaggtgag
     3541 cggctacctg atcataaagg agccgaacgg ctccataggc gcgccgctgc tcatggaagc
     3601 cctccccgag agccgtatga tcttcctgat ccgggatccc agggacgttg tcgcctccag
     3661 catggacgcc cgtagcgagg gaagctggct ctctgagcgg agggaagccc agagaagtac
     3721 gtcgaagcag atcaggaacc cgaacgtcta cgtgaggatg cgcgcgaact cctacgtgca
     3781 acagatagag aagacctggc aggcctacag ggcacacggt ggtcgcaagg tgatggtccg
     3841 ttacgaagac ctgcgcgcag ataccctgca gacgatgaag cgcatctatt cttcgctgga
     3901 gatccccgtg cgagcgaccg atcttgcgcg ctctgtggag aagcactcct gggagaagat
     3961 cccgagcgaa cagaagggcg agggcaagaa gcggcgcaag gcgaagccgg gcggctggag
     4021 ggaggacctg accgcgaggc aggtcgagat agtcgaggat attacggcgc cgctcctgaa
     4081 cgagttctat ccccacacgg cgaccacacg gggcgctctg tgatccggga cgcctggcgc
     4141 aaggtaaggg gtagagggat gctcgaggag aacggcggca tgaggccgga gaacctcgtc
     4201 tgggtgttcg gtacggcgag gacgggaagc tcctggctca gcgccataat gggcgaggta
     4261 ggggggtact gcaggtggca cgagccgctc gtggggcatc tgttcggcaa cctctactac
     4321 gagcgggcgg gccaccgcag cgaggatgag catttcatcc tcggggcccg ccacagggag
     4381 ctctggctcg gtaccataag gaggttcgtc ctcgactcgg ccgccgcgcg tttccccggg
     4441 attgcagcca aaggcggagg gtacctgatc atcaaggagc cgcagggctc gatgggggcg
     4501 ccactgctca tggaggccct gcccgagagt cgtatgatcc tgctcgtccg cgatccgagg
     4561 gacgtcgtcg cctccaacct cgacgcccac aaaggaggga cctggaccgc cgacctgatg
     4621 aggaagggcg gcaaggagaa gccgccttcg ttggccgaga gcaggccgga tgccttcgtg
     4681 aagggccagg caaggaggta cgtgcgggac gtcggaaacg ccaagaaggc ctacgacgcc
     4741 cacggagggc tcaaggtact cgtccgttac gaggacttga ggacggatac tctgcagacg
     4801 atgaagcgca tctactcgga gctggagatg cccgtggagg aggccgatct tgcgcgctct
     4861 gtggagaagc actcctggga gaagatcccg agcgaacaga agggcgaggg caagttccac
     4921 cgcaaggcga agccgggcgg ctggagggaa gatctgaccg caaggcaggc cgagatagtg
     4981 gaaggggtgg caggctccct cctcagagag ctctaccccg tccggagcac cgcgtaggag
     5041 gctcctggtg cccgtcttca gacatcctaa agtctccggt gcggcgcgca ggatcagggc
     5101 cgtccttggc ctcgaggccg gcgttacccg cggagccctg gtagagcgga ccggcaccga
     5161 cgggcgctcc gccggtggtg tcgctcccga gaacatgatc tggatcttcg gcacgggacg
     5221 taccgggagc acctggctcg ccgcgatgat ggaggagccg gaaggtcatg acgtgtggtt
     5281 cgagcccagg gtcggaacca tcttcgaccc ccagaggttc cagcgccacg gcgggaagca
     5341 ctttatcctc gcgtcccagt acaaagacgt ctggctccgt tccatcagga acttcatcct
     5401 ggacggggcc aacgcgcgtt tccccgagct ggacggcgga tacctcgcgg tcaaggaacc
     5461 cggcggctct gtgggtgcgg gcctgatcat ggaggccctg ccggagagtg gtatggtgct
     5521 cctgatccgt gacccgaggg acgtcgtcgc ctcctggctc gacgccacca ggaagggtgg
     5581 ttggcagacc aggcgaaggg gcgaaggggg ccgtcgcgcg gagtctttgg ccgagacgaa
     5641 cccgaacgct ttcgtgaggc gccacgccga cgcctacctg cagcacgtcg ggagcgccag
     5701 gcgggcctac gaggcgcatg agggccgcaa ggtggtggtc cgctacgagg atctgagggc
     5761 cgacaccctg gggacgatca agcgtatgta cggcgagctg aacgtccctg tagaggaggc
     5821 aaagctcgtc aaagccgtgg agaagcacgc ctgggagaac atcccggaag aggagaaggg
     5881 gcggggcaag ttctaccgca aggccactcc cgaggcgtgg cgcgaggacc ttacgcgccg
     5941 ccaggtgaag accgtcgagc ggataacggc gccgcttctt gaggagttct accccactgg
     6001 cccggcggag cagcgaggat gaggagcgag tccgaacgga ggatgcctgt gtcgacctct
     6061 agataccgcg acgcactccg gtccttacgc gaggggtcgg cgcgcctggg accagccgtc
     6121 gacaggctca gggaacgcct cggaaggcaa tcgccaaacg ggacggcctt ccgcgataga
     6181 gagatacccg tgttcttcgt cgtcgggctg gcgaaatcag ggaccacgtg gttgatgaag
     6241 accctggacg cccatcccga agtgctctgc aagggcgagg gccggttctt cggcgaggaa
     6301 tacaggcggg aagtcctggc ccagacgcag accaggcagc agcccagctc actgtacaac
     6361 gcgctgctcg gctccgagta cctgaggctg tggatcgaac ggtcggtgtg gagccgggat
     6421 ggggatcccg acgagcacgt cagggatctc gtcaggctgg ccacagacta cttcctcacg
     6481 caagagctct ccaagagcgg caagaaggtg gtaggggaca agacacccct gctcggcccg
     6541 aagttcgtgg aggagatcta cgagatctac cctgaagcga gggtgatcca catcataagg
     6601 gacggcaggg accaggcggt ctccttcatc caccagcagg gcaaccgcgc caagcggggc
     6661 cgcacgcaca ggctctcgcc ggaggaattg gcgaggagcg aggcttaccg caggagctcg
     6721 cgggacctcg cccagagagg cgaagggatg ttcgccgaga agacactgag gaaagccgcc
     6781 cagaactggg ccctgagggt gggccgggcc atcgaggacg gaccggccct gttcggagac
     6841 cgctacacgg aggtgcgcta cgaagacctg ctggaacgcc ccaacgagga ggtcgggagg
     6901 ttgctcggtt tcttaggggt aaatagggac gaggcgctgg tggagcattg cgtgagctcg
     6961 gcgagcttcc agaagctctc cagggggcgc gagcgaggcc aggaggaccc ctcctctttc
     7021 tatcgtaaag gcgttgcggg ggactggaag aaccactttt cggaggagga caggcgcgtc
     7081 ttcaaggagg aggccgggga actcttgatc aggctcggtt acgagaagaa cggcggctgg
     7141 taggtttgga gggtaggatc aggaaggcga aagcgggcct gcccggtgtg ttgcgcaaag
     7201 gccgtcgctg gctggccggc aagtacaggg aggtccccct tctggcggcc tcgctgcgga
     7261 ggaggttcga tggcggtcct gccagcggcg taaggccgga gaacatcgtc tggatcttcg
     7321 gctcggggcg cagcggcagc acgtggctca ggagcatgat gggagatccc agccggcatc
     7381 tggtctggga ggagcccatg gtgggccagc tcttcggcaa cttccaccgc agggcagaga
     7441 aagcgaacct catccgtccg gacttcgtca tggccgacgc gacccgtagg ggttggatcc
     7501 actccataag gaacttcgtg ctcgacggcg cacgctacgc aagaccccgc ctcggtcccg
     7561 accattacct cataatcaag gagccgaacg gctcggtcgg ggctcccctg ctcatggaag
     7621 ctctccccga gagccgcatg gtactcctga tccgggaccc gagagacgtg gtctcgtcga
     7681 cgctcgacgg ggcccgcaag ggaaactggc tctacgagcg caaggacaag ggcggatgga
     7741 agcgcgattc gctgccggac gaagaccccg acgcgttcac caggcgctgg gcgaacgtct
     7801 atatgcaaca cgcggggaat gccaagatgg cctacgacgc ccacagaggt ccgaaagtca
     7861 tggtccgcta cgaagacctc agggcagaca ctctgggcac catgcggcgt atctactcga
     7921 cgctcggagt acccgtggac gaacacgaac tggcacgggc tgtggagaag cactcctggg
     7981 agaacatccc ggagaagaag aagggcgggg gcaaattcta ccgcaaggca acaccaggag
     8041 gctggcagga ggacctcact ccaaagcaga taaagcaggt cgaaaggata acggcccccc
     8101 tgctcgaaga gtattattct ggcagaacgc tgtaacattg gctgagaagg tctcggtcgt
     8161 catacccacg ttcaatgcgg gcccagcatt cggggagctg ctgcgcaggc tcgacgccca
     8221 ggagggagat ttcgagcggg aggtcatcgt cgtcgactcg ggctcgaccg acggcacagc
     8281 gcagctcgcc gcaaggcaag gcgcgatctt gcacaacgtt tcgaaagcca gtttcgacca
     8341 cggcgcgacg cgggacctcg ggatctcgct ctcatccgga gaatacgtgg cccttacggt
     8401 tcaagacgca gtgccgctcg acgcacggtg gctggtcacc atggtcgaga acctgcaaag
     8461 tgacggtcgg atcgccgcgg tctacggccg ccacgtcccc aggcccgacg ccggcgcaat
     8521 gaccagggcg ctggtcggca acctcgccgt cgcaaacacc gaaaggcggg agcaggagat
     8581 ccccgacacg gctcagtacc tgtcgctgcc accgtcccag aggcgccgca tcgccgcctt
     8641 cgacaacgtg agctcctgcc tgcgccgctc ggtctgggag gagttccctt tcggggaggc
     8701 ggacttcgcc gaggacctgc gttggggcaa gaaggtggtg gaggccgggt acaccatcgt
     8761 ctacgagccg cgctcggtcg tcgtccactc ccacgagcgc ggcgccctct acgacctcag
     8821 gcgccactac gtagaccagc tcgtcctcga cgagctcttc ggggcccgcc tggtgcctaa
     8881 cctgccgcgc ctgatcctcg gcatacccta cgcaacccac cacgtctacg gccttctcag
     8941 gcgcgaggaa ccggcccccg aaaggaggcc gaagctcctg ctcgacgcgg caactttcgc
     9001 cctcgcatcc cagctcggcg cctacctggg atgcaagctc ccttccctct cccaaccgac
     9061 cccgaaactc cacgccaggc tccaccgctt cctcggcaaa ggagtgtgac gcggcccggg
     9121 gcctcccggg ccgcgagcat gtcagcacgc cgccttcggc ggcttgtcag catttcagct
     9181 ggtcagctac ctgtagcgtg gtgtgaagtc tgtgcgcttg cgagaatccc agaggagccc
     9241 tactcataga gagtcctctc cgactgcaga acctgacaag ctgaaatgct gaagtgctga
     9301 cgagctaagg cctgaaaggc ctgagcgtgc tgaaatgctg accgctataa tctatgccat
     9361 gaacctcgaa cgcgtcgtgg agcgccttcg cgactaccgg ctgaagaacg agcgattcga
     9421 ccagctggtc ggggctgtgc tctccaacga gaaggccatg agggccgcga gcgccacagt
     9481 cgcagcggcc cgctccgtct cccgtagcgg ccagaccgtc gccgcgggag attacgacac
     9541 ggtgttcttc gtggcgggag agatgcgctc ggggacctcc tggttgcgcc acagcctgag
     9601 cgaccacccc gagatcgcct gcggccagga aggcagcttc ttcggccgcg gatacgacca
     9661 cgaggagata cccgtctatg cggggccagt ctccagcctc acccgcgccc tcgcggtctc
     9721 gagggagttg aagacctggc acgcgctgcc gtggaacctg tggtcggacg gatacgacga
     9781 agacttgagg aacctctctc gcctctgtgt agactacttt ctctgcaaag aggtggcccg
     9841 cacgggacgc aggatagtgg gtgacaagag cccccagcac acggagaatc tggacgagat
     9901 atacgagatc taccccgacg ccaggataat ccacatcatc agggacggca gggatgtggc
     9961 ggtctcggcg atgcaccact ggtggcggct ggccagggac agggaggggg gcgtcttcga
    10021 gctctcgccg gaagagctcc gcatccgcga cgactacgcc gaagacaggg agcggttcct
    10081 gtccgagggg cgttcgatct tcacgcggga gcggctcgcc cagctggcca gccggtggga
    10141 gtacaggacg gggaaaggca gccacgacgg gagaaccctc tacggtgaga gatacctgga
    10201 gatccggtac gaagacctcc tcgtcgatac gaactcggtc ttccggaaga tcctcgagtt
    10261 cctgggggcc ggcagcgagg accgggtaat agagcgctgc ctgcggtcga gcagcttcga
    10321 gcgggcctcg agccgcaggc agggcgagga ggattcggcc tctttcttca gaaggggcgt
    10381 cgccggcgac tggaggaatg tcttcacgga gcgagaccgg gaactctata aacagctggc
    10441 cggggagcga ttggtggagt tcgggtacga gaagtcgtat gactggtagg gcgtgaggat
    10501 cctcttcgcc gcccaccagt tcttccccga gcattacgcc ggcgtcgaga ccgtcacgct
    10561 ctcgctggca caggagttca aggcgagggg ccacgagccc tacgtgctgg cccccaagcg
    10621 gagcatccct ggcaacgaca tcgagccggg agaggtcgaa gactacgaat tcgggggcat
    10681 accggtgcgc agggtgggcc gtcccaaaga gggcgtctcc cgcccctaca gcctggatta
    10741 cgagaacggg gtcatggccc ttaggacccg cgagtacctg cggaagatat cccccgacat
    10801 cgtacatgcc gagcacttcc agggactctc tgcgagcgtg atccccgcta tcaaggagtt
    10861 cggcgtgccg ctcgtgtaca cggcgacgga tttctggacg ttctgccccg tcgtcgatct
    10921 cagacgccac gacggggtga tgtgcagggg gcccgagatc tcgcactgca cccgttgcat
    10981 agcgagcaga taccccggca ccaggatgaa gcaggccgtc gacctgacgc ccccggccgc
    11041 cataaagctg gccggcgccc tctcggagac caggctctcg gggctctccc actcgctgcg
    11101 ccaggtgagg gccctaaagg agcgcccgga gcgcataagg gagaatatgg ggctggtcga
    11161 ccacgttatc gtctacaccc acctcatgcg ggagctgttg ctcgccaacg gcattggagc
    11221 gggcaagatc gaggtctccc actacggcat agacacctcg ggcatacgca gggaagacag
    11281 gcccttctcc ccgccgctcc gggtcggctt tgtgggaacc ctggcgccgc acaagggctg
    11341 cgacgtcctg atccgggcct tcagagacct cccgccggag gtggacgcga ccctcgatat
    11401 ctacggtaac ctggagaggt ttaaaccctt tgtggaagag ctgcgcgccc tggccggggg
    11461 agacggtagg atagatttcg ccggcccctt tgcgcgcgag gaggtaggcg acgtcctctc
    11521 ggggatggac gtccttgtcg tgccctcccg ctggtacgag aacggtccag gggtgatctt
    11581 cgaggctttc gccgccggta tcccggtggt ggcgacgaac ctcggcggga tgtccgagtt
    11641 cgtgaggcac gagagagacg ggctgctctt cgggctggag gaccacggcg ggctcgccag
    11701 ccagctcaga cgcctcgtcg aggaaccggg cctgctcgag aggctccgag ccgggatcga
    11761 accggtcaag accgtccagg agaacgtcga cgagctcgag gtcttgtacg gcgggctgct
    11821 ggcgagggtc tagacagagt gccgaagttc ctcgtaagcg gctcgggcgg ccagctcggc
    11881 ctcgagctgg ccgcgctcct cccccggcgc ggccacgagg tcatcgcgct ctcgaaggga
    11941 gagctcgacg tggcggactt cggtgccgtg cggctggcgt tggaggagca cgctccggac
    12001 atcgtcgtca acgcggccgc ctacacgaac gtcgacggct gcgagtcaga ggccgacctt
    12061 gcttacaggg tcaacgcgct cggtccgagg aacctgg
//
LOCUS       DATMVF010000019        11238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_277393, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000019 DATMVF010000000
VERSION     DATMVF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277393"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1076)
                     /locus_tag="VN178_00615"
     CDS             complement(102..1076)
                     /locus_tag="VN178_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HWS79492.1"
                     /translation="MAIRVKSPGLLTTVQDTGRLGEYAIGMPPSGAMDVFSYQVGNYL
                     VGNEDGAAGLEMTYLGPELEFTESSVIAITGAEMPPKINGEEAPTWETLAVESGDVLT
                     FDYLRNGARAYLAVAGGIDVPVFMHSRSTYTLIGLGGHEGRALAEGDELAVGEAGDRS
                     DRVGMAVVEQRIPQYSKETELRVIIGLASYRLTEESMEEFLSTTWTVTPDADRVGYRY
                     KGGELGFVEREQPAGAGADQANVVDFGYPVGSIQVPGGVEPIVLMNDAVTGGGYATIG
                     TVISADRDKLAQTKTNDKTRFRSVDLEEALAAREHRRKQMAEIKRALS"
     gene            complement(1066..1983)
                     /locus_tag="VN178_00620"
     CDS             complement(1066..1983)
                     /locus_tag="VN178_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HWS79493.1"
                     /translation="MQPNMSRGIETELPQARYSYGGDEFVFVELAEAMSLKVNFRAMA
                     ITNRLQEERINGVMDICPSNASYMVRIDPDVLHPDDLISRLKEIDAEAGDIQGFELQT
                     RVVDVPVMMEDPWTHETLMRFRDRHQDPETTDLEYSARINGYDSKEDFIAALLGSPWL
                     VTMIGFVPGLPWCYQLVPPEEQVEVPKYVRPRTYTPERAFGFGGGFAVVYSVEGAGGY
                     QLYGLAPAPVLDVKQELKDFQDSIVFPRPGDIFNYRGIDRGEFDEIRGRVEDGTFEYR
                     KKEFVFHPDRALDDPHAYNEEILGVLYGD"
     gene            2088..2636
                     /locus_tag="VN178_00625"
     CDS             2088..2636
                     /locus_tag="VN178_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HWS79494.1"
                     /translation="MNAATMTRVALMAAVTAVAAQIAIPLPFSPVPFTLQVPAVVLSG
                     LLLGSRHGALAMAVYLLVGAIGVPVFAQFSGGLGRIVGPTGGYLLSYPLAAAVAGLAA
                     YAAANSPRSRALMLSFLWGVAALAVIYAIGAGWLSIVANLPIAVAVAQGVLPFVVFDL
                     VKIVLAALVAVAAAPAIAGSRI"
     gene            2708..4048
                     /gene="accC"
                     /locus_tag="VN178_00630"
     CDS             2708..4048
                     /gene="accC"
                     /locus_tag="VN178_00630"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWS79495.1"
                     /translation="MFEKVLVANRGEISLRVVRACQELGVAAVAVYSDADESALHVRH
                     ADEAVRIGPPPAGKSYLNIEALLEAARETGAEAIHPGYGFLAENARFAAACREAGLTF
                     VGPSAEAIEKMGNKSAARRLAREAKVPVVPGSDDASSAEEAAQTAGGIGYPIMVKAAA
                     GGGGRGIRVAEDEDELRKAVQVARREAEAAFGDGTLYLEKLLVAPRHVEVQVMGDHEG
                     SVIHLYERECSMQRRRQKVLEEAPSPGISQGVREKMAEAAVRLAREAGYTNAGTVEFL
                     VEDEEYYFIEMNTRIQVEHPVTEMLTGVDLVKEQIRIAAGDPLSLEQEDVPMVGHAME
                     FRINAEDPDQDFMPSPGEISSLEVPGGPGVRVDSAIYQGYQIPPFYDSMVGKLVVWAL
                     TREEAISRARRALREYRLEGINTTIPLHLRLLEGEAFRSGEYHTGYLEELLNGG"
     gene            4128..4277
                     /locus_tag="VN178_00635"
     CDS             4128..4277
                     /locus_tag="VN178_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79496.1"
                     /translation="MRTIPMEVKRRRFTVHDYHRMGEAGILHEETLESDALPGPTPSV
                     DEVLG"
     gene            complement(4279..5619)
                     /gene="gabT"
                     /locus_tag="VN178_00640"
     CDS             complement(4279..5619)
                     /gene="gabT"
                     /locus_tag="VN178_00640"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HWS79497.1"
                     /translation="MLESTRIKTEIPGPKSRALMERRHSAVSSGVGIATPMVAKEAKG
                     ALLTDVDGNTYIDFGGGIGVMNVGHTDSRVVEAVKEQAERLTHSCFYVTEYEEYIELA
                     EKLNALVPGDTEKRSFFVNSGAEAVENAAKIARYYTKRPAIIAFENSFHGRTMFAMTL
                     TGKTDPYKKGFGPFVPEVYRVPAPYAYRCPAGRDCSGGCVGDCFASIEKAFVSTVHPS
                     SVAAIIVEPVSGEGGFIPFPDFYLRRLRELCDEHGILMIVDEVQTGFGRTGKMLAVEH
                     SGVEPDILTTAKSLGGGLPIAGVTGKKEIMDSVHVGGLGTTYGGNPIACAAALAVFEA
                     FKEEDLLARANVLGERVMAAMREIQDRHPDFVGDVRGRGPMAAMEFVTDAESRTPDKE
                     RTAKVISAALQEGLLLLSAGQYGNCIRTLMPFVIADEELEEGLAILGRAVDAAA"
     gene            complement(5989..7422)
                     /locus_tag="VN178_00645"
     CDS             complement(5989..7422)
                     /locus_tag="VN178_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWS79498.1"
                     /translation="MAQAGVEGREQGQQQGHLQQGLKRRHMTMISLGGIIGAGLFVGT
                     GPILNQAGPATILTYLFTGCILILIMRMLGEMAVALPTVGSFSDYSRMALGNWAGFSI
                     GWLYWYFWAIVVGFEATVAAGILGQYVNVAPWLISLILVLALTATNLYSVGSYGEFEF
                     WFAGIKVAAIVIFIVLGALFVIGLWPGGGGGMGLSNLYDQGGFFPNGGLVMFSGIVTV
                     IFAFVGAEIVTIAASESNEPSRNVARATNAVIYRVLLFYVVSVFFVAAIVPWDTTFAE
                     DVIKSPFTLAFERMGIPGAGTLMNFVVLTAVLSVLNSSLYTTSRMLFALTRHNDAPGF
                     FTNTTARGVPIWAILAGTSFGYVSVAIYYFFPANVFTWLINASGAIALFAYLLIAISE
                     LVMRRRLERENPESLQLRMWFYPWLTYLSIAAMVAVLVAMFIIPDQRPLLIASLVSLG
                     VILAAYMLRRWFGPPERSPSEIIREHD"
     gene            complement(7509..8663)
                     /locus_tag="VN178_00650"
     CDS             complement(7509..8663)
                     /locus_tag="VN178_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWS79499.1"
                     /translation="MSVHFAAEELAARRKSASEELARRGLDGLLMFRQESMYYLTGYD
                     TFGYVFFQCLYMGADGETLTLLTRTPDVRQAEHTSVVEDIRVWYDAEGADPAGDLREI
                     LEEHGCRGKRLGIETDAYGLTGYNLKRVETALDGFCELVEASDLVSRLRVVKSPAEIA
                     YVREAARLADLGLDRAVEVAAPGVFEGDVLAALQGAIFEGGGDFPANEVIIGSGPRAL
                     LFRSAAGMRRMDPIDQLSLEWAGSYRHYHAAMMRTLVVGEASSYQRHLFSAVREALEE
                     MTGALAPGRPVGEVDDAHRRVLDAAGLEEHRFAACGYSLGTTFSPNWMDWPMLFSGNP
                     VLAEAGMVFFLHCVVADSGRGVAMSLGHTCLVTASGREVLSARPLELIVV"
     gene            complement(8682..9914)
                     /locus_tag="VN178_00655"
     CDS             complement(8682..9914)
                     /locus_tag="VN178_00655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS79500.1"
                     /translation="MSRGASVAIVGGGIWGLSTAYHLARAGWTDVQVLERNDALFDET
                     TSQAAGLVGQIRATPLMRRAIRYAIDLFLDFGRETGHDPGFRQVGSLLLALTPERLAS
                     FEEHVEHANRSGVEARFLDGAQMSRLAPHLDVSRVEGGYFVPEDGYLDPQACARAFCG
                     AAEDLGVEIRTGTAVTELSIKDGRVMGVETAGGFVEAEYVVVTAGPWTGTIAEMAGHR
                     TAMVPIRHQRVTTAPVSDIPDHHPVVRVTDASCYLRPEEGGYLYGFFEPDPKSYDPEL
                     PAGFHTAEIEEPIVVMEEARERLTPVFPVLKDLEIADYKRGLTTFAPDGAYLIGPVPQ
                     VEGLFAATGCAALGIAGSAAVGNWLAGWIAQDDPGEDLSSVGLERFGEKASDRAWIRR
                     ESEAFYGGYYGIPAMSVT"
     gene            complement(9954..11075)
                     /gene="ald"
                     /locus_tag="VN178_00660"
     CDS             complement(9954..11075)
                     /gene="ald"
                     /locus_tag="VN178_00660"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWS79501.1"
                     /translation="MATVIGVPKEIKDMEGRVSMQPDGVAELVHYGNEVYVQAGAGEG
                     SGFSDEEYDEAGARLVDGPDEVFESSDLIVKVKEPVPEEYDRFREGQQLFTYLHLAAD
                     KGLTEFLMERRINSIAYETVEASDGSLPLLTPMSEVAGRMSVQAAAHHLESPQGGAGL
                     LLGGVPGTPAAKVTIIGGGVVGTEAAKIALGMRALVSVLDISPKRLAYLSDIFEGRAD
                     LVIPNRARTAAYVRQADVVIGAVLVHGAKAPKLVTREMVASMRPGSVVADVAIDQGGC
                     VETARPTTHSDPTYVEEGVVHYCVANIPGAVARTSTLALTSATLPYLIKIADKGIEGA
                     ATEDESLAKGLSTLRGDLVSEPVAEAHDLSYTDAQKLLN"
BASE COUNT         2011 a         3656 c         3526 g         2045 t
ORIGIN      
        1 ttctctccgg ccaggacaga ccctcctgct cgccccgagt ggcgcatcgc ttgctccacc
       61 cagaatcgtc ccgcaactcc tgatgcctgt agtctgaaac ctcaagaaag cgctctcttg
      121 atctccgcca tctgtttgcg cctgtgctct cgggccgcga gcgcttcttc gaggtcgacg
      181 gaacggaatc gggtcttgtc gttggtcttg gtctgggcca gtttgtcgcg gtccgcgctt
      241 atgacggtgc ctatggtcgc gtagcccccg ccggtgacgg cgtcgttcat cagtacgatg
      301 ggctcgacac cgccaggcac ctggatggag ccgacgggat agccgaagtc aaccacgtta
      361 gcctggtctg cccctgcacc ggccggctgt tcccgctcga cgaaacctag ttcgccgccc
      421 ttatagcggt agccgacccg gtcagcgtcg ggggtgaccg tccacgtagt gctcaggaac
      481 tcctccatgc tctcctcggt caaccggtag ctggcgagcc cgataatgac gcgcagctct
      541 gtctctttgg agtattgtgg gatccgctgc tccacgacgg ccatcccaac gcggtcggag
      601 cggtcaccag cctcgcccac cgccagctcg tcgccctcgg ccagcgccct gccctcgtgt
      661 cccccaagcc cgatcagggt gtaggtcgag cggctgtgca tgaagacggg cacgtctatc
      721 ccgcccgcca cggccaggta agctctcgct ccgttccgga gatagtcgaa ggtgagcacg
      781 tcacccgact ccacggcgag cgtctcccag gtaggggcct cctcgccgtt tatcttcggc
      841 ggcatctcgg ccccggtgat ggcgatgacg gagctttctg tgaattctag ctccgggccg
      901 aggtacgtca tctccaaacc cgccgcgccg tcctcgttgc ccaccaggta gttaccgacc
      961 tggtaggaga aaacatccat agcccccgag ggcggcatcc cgatagcgta ttcgccgagg
     1021 cggcccgtgt cctggacggt ggtgagtaga ccagggctct tcactctaat cgccatagag
     1081 tacccccagg atctcctcgt tatacgcgtg ggggtcgtcg agcgcgcgat cggggtggaa
     1141 gacgaactcc ttctttcggt actcgaaggt gccgtcctcg acgcggcccc ggatctcgtc
     1201 gaactccccc ctgtctatgc cacggtagtt gaaaatgtca cccggtctcg ggaagactat
     1261 cgagtcctga aagtccttta gctcctgctt cacgtcgagg acgggcgcag gggcgaggcc
     1321 gtaaagctgg taaccgccgg ccccttcgac cgagtagacg acggcgaacc cgccgccgaa
     1381 gccgaaggcc cgttccgggg tgtaggtgcg gggcctcacg tacttgggca cctccacctg
     1441 ctcctccggt gggacgagct ggtagcacca cggtaggcca ggcacgaacc cgatcatggt
     1501 cacgagccaa ggcgaaccaa ggagcgccgc gataaagtcc tccttcgagt cgtagccatt
     1561 aatgcgggca gagtactcga ggtcggtcgt ttcagggtcc tgatggcgat cacgaaagcg
     1621 catcagggtc tcgtgggtcc aggggtcttc catcattacg ggcacgtcaa cgacccgcgt
     1681 ctggagctcg aatccctgaa tatctccggc ttcggcgtct atctccttga ggcgtgagat
     1741 caggtcgtca gggtgcagca cgtcaggatc tatgcggacc atgtacgagg cgttggacgg
     1801 acagatgtcc ataacgccat tgatgcgttc ctcctggagc ctgttcgtta tcgccatagc
     1861 cctgaagttg accttcaggc tcatcgcttc ggccagttcg acgaagacga actcatcccc
     1921 cccgtagctg tagcgcgctt gcggaagctc ggtctcgatg ccgcgactca tattcggttg
     1981 caccccgcct tctcctggac tgtgccgcaa tactacatac gccggatttt tcggtcaata
     2041 gatgctaaaa gggagccggg atgaattcga gggtgaggga ggcgaacttg aacgcagcta
     2101 cgatgacccg cgtggcgctc atggccgccg tcaccgccgt ggcagcccag atcgctatcc
     2161 cgctgccttt ctcgcccgtg ccgttcacgc tacaggtgcc ggcggtcgtc ctctccgggc
     2221 tgctgctcgg ctcgcgccac ggggcgctgg cgatggcagt ctacctactc gtcggggcca
     2281 ttggcgtgcc cgtgttcgcc cagttctcgg gcgggctcgg caggatcgta ggacctacag
     2341 gcgggtacct cctctcctat ccactggctg ccgccgtggc cggcctcgcc gcctacgccg
     2401 cggcaaactc gccccgtagc agagcgctaa tgctgagctt tctgtggggc gttgcagccc
     2461 tagccgttat ctacgctatc ggggctggct ggctctcgat agtagcaaac ctgcccatcg
     2521 cggtagccgt cgcccagggc gtgctgcctt tcgtggtctt cgacctggtg aagatcgtcc
     2581 tggcggcgct cgtcgccgtg gccgcagccc cggctatcgc cggctcccgg atctgaggga
     2641 gcgcgggacc cggagtgggg ctaatatgac gttccggtcg tggtggttgg aaggaggtcg
     2701 agatatcgtg ttcgagaagg ttctcgtggc taatcggggg gagatctcgc ttcgcgtcgt
     2761 gcgcgcctgc caggagctcg gggtggcggc cgtcgccgtc tactccgacg ccgatgagag
     2821 cgccctccac gtccgccacg ccgacgaggc tgtccgcatc ggcccgccgc ccgctggcaa
     2881 gagctacctg aatatagaag cgttgctcga agccgcaaga gagacggggg cggaggccat
     2941 ccaccccggc tacggttttc tggcggagaa tgcgcgtttc gcggcggcct gccgggaggc
     3001 cgggctcacg ttcgtcgggc cttcggccga ggcaatcgag aagatgggga acaagtccgc
     3061 cgcgcgtcgg ctagctcgtg aggccaaggt gccggtcgtg cccggttcgg acgatgcctc
     3121 ttccgctgaa gaggcggcac agacggctgg tgggatagga tacccgatca tggtcaaggc
     3181 agcggcgggt ggcggtggcc gcggcatcag ggtggctgag gatgaggacg agctcaggaa
     3241 ggccgtacag gtggccaggc gcgaggccga ggccgctttt ggggacggga cgctctacct
     3301 ggagaagctg ctcgtcgcgc cgaggcacgt cgaagtgcag gtcatgggcg accacgaggg
     3361 gagcgtgatc cacctctacg agcgcgagtg ctcgatgcaa cgccggcgac agaaggtgct
     3421 cgaagaggcc ccctcgccag gtataagcca aggggttcgc gaaaagatgg ctgaggcggc
     3481 ggttcggttg gccagggaag ctgggtacac gaacgctggt accgtggagt ttttggtcga
     3541 ggacgaagag tactatttta tagagatgaa cacgcgcatc caggtcgagc accccgtgac
     3601 cgagatgctc accggcgtgg atctggtgaa ggagcagatt cggatagctg cgggagaccc
     3661 cttgagcctc gaacaggaag acgtgccgat ggtcgggcac gcgatggagt ttcggatcaa
     3721 cgccgaggac cccgaccagg actttatgcc gtctccaggc gagatctcct cgctcgaagt
     3781 cccaggcggc cccggcgtca gggtagactc ggccatctat caaggctacc agattccccc
     3841 gttctacgac tcgatggtgg gcaagctcgt agtctgggcc ctcaccagag aggaagccat
     3901 cagcagggcg agacgcgccc tccgcgaata ccgcctcgaa ggcataaaca cgacgatccc
     3961 cctccacctc aggctcctgg aaggagaagc cttcagatcc ggtgaatacc acacgggcta
     4021 cctagaagag ctgttgaacg gaggctgaaa aggacggaaa cctaaaggtt tctcttcgcg
     4081 ctccgcgtat actcccgcat gagacgagcg agttaccgga ggattgcgtg cggacgatac
     4141 cgatggaggt caagcggcgg cgtttcaccg ttcacgacta ccaccgcatg ggcgaggccg
     4201 gcatcctgca cgaggagacg ctggagtccg atgcgctgcc gggcccgacc ccgagcgtgg
     4261 acgaagttct cggctgattc aggctgcggc gtctacggcg cgtcccagga tggcgagtcc
     4321 ctcttccagt tcctcgtcgg cgatgacgaa gggcatgagg gttcggatgc agttgccgta
     4381 ctggccggcg ctgaggagca ggagcccttc ctggagggcg gccgagatca ccttcgcggt
     4441 cctctccttg tcgggggtgc ggctctctgc gtccgtgacg aactccatcg cggccatcgg
     4501 gcccctgccc cgcacgtcgc ccacgaagtc ggggtgacgg tcctggatct cgcgcatcgc
     4561 ggccatgacg cgttcgccga gtacgttcgc acgcgcgagg agatcttcct ccttgaaggc
     4621 ttcgaatacc gctagggcgg cggcgcaggc tatggggttg ccgccgtagg tggtgcccag
     4681 gccaccgacg tggacggagt ccatgatctc cttcttcccc gtaacgccgg ctatggggag
     4741 tccaccgccc aggctcttgg ccgtggtgag gatgtcaggc tcgacccccg agtgctcgac
     4801 ggcgagcatc ttgcccgtcc tgccgaaacc agtctggacc tcgtcgacga tcatcaggat
     4861 gccatgctcg tcgcacagct cccgcagcct cctcaggtag aaatccggga acggtataaa
     4921 gccgccctca ccgctcaccg gctcgacgat gatcgccgcg acgctgctag ggtgcaccgt
     4981 gctcacgaac gccttctcga tggaggcgaa gcagtcgccc acgcagccgc cggagcagtc
     5041 cctgccagca gggcagcggt acgcgtaggg cgccggtacc ctgtagacct cggggacgaa
     5101 aggcccgaag cctttcttgt acgggtccgt cttccccgtg agggtcatag cgaacatggt
     5161 gcgtccgtgg aaggagttct cgaaggctat tatcgcagga cgcttcgtgt agtagcgtgc
     5221 gatcttggcc gcgttctcga cagcctcggc ccccgagttg acgaagaacg agcgcttctc
     5281 cgtgtcgccc ggcaccagcg cattcagctt ctcggccagc tctatgtatt cctcgtactc
     5341 ggtgacgtag aagcagctgt gggtgagcct ctcggcctgc tccttgaccg cctcgaccac
     5401 ccgcgagtcc gtgtgcccga cgttcatcac cccgatgccg ccaccgaagt ctatgtacgt
     5461 gttgccgtcc acgtcggtga gcaaggcgcc cttcgcttcc ttcgcgacca tcggcgtagc
     5521 gatcccaacc cctgaggaga cagcgctgtg ccgccgctcc atcaaagccc ggctcttcgg
     5581 gcccggtatc tcggtcttga tcctcgtgga ctcgagcata gtcgccacct cttcctgtcg
     5641 ggctaattcg ttccagtata ctccccaccg gctaccgagc gatacccctg acagcgggtt
     5701 gcaggaggat ggacttcctg caacccgcgc cgtcagcact aagctttcag ccttcagctt
     5761 tttgttcttg ctgatcgctg aaagccaata gctcctcgtg cataagggta gggcccttag
     5821 gcacggcgct gtttgtcaga cagtagagcg tgtcgtttgc atcggccacg actttcttcg
     5881 agaacgcgaa caggctgagg aactcgtgcg cgaggcagag ctgcgcgcgt tctcgaagga
     5941 gtcggcaggt tcgtagcgat tcgagaggcc ctctgttcag gaggttgcct agtcatgctc
     6001 cctgattatc tcactggggg acctctccgg cggtccgaac cagcggcgga gcatgtaggc
     6061 tgccaggatc acgccgaggc tgacgaggct ggcgatcagc aagggacgct ggtcggggat
     6121 gatgaacatg gcgacgagca ccgcaaccat ggcggctatg gacaggtacg tcagccacgg
     6181 gtagaaccac atcctcaact ggagcgactc cggattctcg cgctccaggc ggcggcgcat
     6241 gacgagctcc gagatggcga tcaggaggta ggcgaagagc gcgatcgccc ccgaggcgtt
     6301 gatcagccac gtgaagacgt tcgccgggaa gaagtagtag atcgcgacgg acacgtaccc
     6361 gaagctcgtc ccggccagga tcgcccagat tggcacgccg cgtgccgtcg tgttggtgaa
     6421 gaaccccggc gcgtcgttgt gcctggtaag ggcgaagagc atccgcgagg tggtgtacag
     6481 gctcgagttg aggacggaga gcacggcggt caggaccacg aagttcatca gcgtaccggc
     6541 tcccggtatc cccattctct cgaacgcgag ggtgaacggg ctcttgatga catcttcggc
     6601 gaaagtggtg tcccacggca cgatggcggc gacgaagaag accgagacca cgtagaagag
     6661 caggaccctg tagatgaccg cgttggtcgc gcgggccacg ttcctgctcg gctcgttgga
     6721 ctcagacgcc gcgatggtga cgatctccgc cccgacgaac gcgaagatca cggtgacgat
     6781 gccgctgaac atcaccagac cgccgttggg gaagaagccg ccctggtcgt agaggttcga
     6841 gagccccatg cccccgcctc cccccggcca cagcccgatc acgaacagcg cacctagcac
     6901 gataaagatg acgatcgcgg cgacctttat ccccgcgaac cagaactcga actcgccgta
     6961 ggagccgacg gagtagaggt tggtggcggt gagcgccagg accaggatca gggatatgag
     7021 ccacggcgct acgttcacgt actgtccgag gatgccggcc gctaccgtgg cctcgaaccc
     7081 gaccacgatg gcccagaagt accagtagag ccagccgatg gagaacccgg cccagttacc
     7141 cagggccatc cggctatagt cggagaagga gccgaccgtc ggcagcgcca cggccatctc
     7201 gccgagcata cgcatgatca ggatcaagat gcaccccgtg aaaaggtacg tcaggatggt
     7261 cgcaggacct gcctggttga ggatcggacc ggtcccgacg aacaacccgg ccccgatgat
     7321 cccgccgagg gagatcatgg tcatgtgccg cctcttcaaa ccctgttgca ggtggccctg
     7381 ctgctgtcct tgctctcgtc cttccacacc cgcttgggcc atgggattcc ccttccgtga
     7441 gatcgctgcc ttgcgcgcag cttacaacag gctacacacc atttctttca acgaatcgga
     7501 agtccggctc agaccacgat caactccaga ggccgcgcgg agagaacctc cctcccggaa
     7561 gcggtcacca ggcaggtgtg gcccaggctc atggccaccc cgcgccccga atcggccacc
     7621 acgcaatgca agaagaagac catcccagcc tcggccaaca cagggttgcc cgagaacagc
     7681 atcggccagt ccatccagtt gggactgaag gttgtgccta aagaataccc gcacgcggcg
     7741 aagcgatgct cttcgagccc cgccgcgtcc agtacgcggc gatgggcatc gtcgacctcg
     7801 ccgaccggac gtcctggcgc cagggccccg gtcatctcct cgagcgcctc gcgaacggcg
     7861 gagaagaggt ggcgctggta actggaggcc tcgcccacta cgagggtacg catcatggcg
     7921 gcgtgatagt gcctgtagga accggcccac tccagagata actggtcgat agggtccatg
     7981 cggcgcatgc cggcggccga gcggaacaag agggcgcggg gtccggagcc gattatgacc
     8041 tcgttcgccg ggaagtcccc gccgccctcg aagatggccc cctgcaaagc cgccaggaca
     8101 tccccctcga agacgccagg cgcagctacc tccacggccc tgtccaggcc gagatccgcg
     8161 agtcgtgcgg cttcccgcac gtaggcgatc tcggcggggg acttcactac ccgcaacctg
     8221 ctcacgagat cggaagcctc caccagctcg cagaagccat cgagcgcagt ctccacgcgc
     8281 ttgaggttgt agcccgtgag accgtaggca tcggtctcta tccccaggcg cttcccccta
     8341 cagccgtgct cttcgaggat ctcgcgcagg tcaccggcgg gatctgcgcc ttctgcgtcg
     8401 taccagaccc tgatgtcctc gactaccgag gtgtgctcgg cctgccgcac gtccggggtg
     8461 cgggtcagga gggtgagggt ctcgccatcg gcacccatgt agaggcactg gaagaagacg
     8521 tagccgaatg tgtcatatcc cgtcaggtag tacatcgact cctggcggaa catgagtagt
     8581 ccgtcgaggc cgcgcctcgc caactcttcg gacgcggact tacggcgggc tgccagttct
     8641 tccgcagcga agtgcactga cacggttaca ggctccccga gctaggttac ggacatggcc
     8701 ggaataccgt aatagccgcc gtagaacgct tcgctctcac gcctgatcca ggctcggtcc
     8761 gatgccttct ctccgaaccg ctccaggccg accgatgaga ggtcttctcc cgggtcatcc
     8821 tgagcgatcc agccggccag ccagtttcct accgcggcgg agcccgcgat accaagagca
     8881 gcgcatcctg tggcggcgaa gaggccctcc acctgcggca cgggcccgat caggtaagcc
     8941 ccgtcagggg cgaaggtggt aagaccacgc ttgtagtcgg ctatctccaa gtctttaagc
     9001 accggaaaga cgggagtcaa cctctcgcgg gcctcttcca tcaccactat gggctcctcg
     9061 atctccgccg tgtggaatcc ggccggcaac tcggggtcgt agctctttgg atccggctcg
     9121 aagaacccgt agaggtaccc tccctcctcg ggcctcagat agcaactcgc gtcggtgacg
     9181 cggacaaccg ggtggtgatc cgggatgtcc gaaacagggg ccgtggtaac gcgctggtgg
     9241 cgtatgggca ccatcgccgt acgatgaccc gccatctcgg cgatggtccc cgtccagggg
     9301 ccggccgtca cgacgacgta ctccgcctcg acgaaaccgc cggcggtctc gacccccatc
     9361 acccggccgt cctttatcga gagttcggtt actgctgtgc ccgtcctgat ctcgacgccc
     9421 agatcctctg cggcgccgca gaaggcccgc gcgcatgctt ggggatctag gtagccgtcc
     9481 tcaggaacga agtacccgcc ctcgacccgc gagacgtcca ggtgcggagc cagtctggac
     9541 atctgggcac cgtcaaggaa gcgggcctcc acgccgctcc tgttcgcgtg ttctacgtgc
     9601 tcctcgaaag aggccagccg ctccggagtg agggccagta ggagactgcc gacctggcgg
     9661 aaaccggggt cgtggccggt ctccctgccg aagtccaaga acaggtctat ggcgtaccgg
     9721 atcgcccgtc tcatgagtgg ggtggcccgg atctggccga ccaacccggc ggcctgggag
     9781 gtcgtctcgt cgaacagagc atcgttacgc tccaggacct gcacgtccgt ccatccggct
     9841 cgcgccaggt ggtaggcggt gctcaacccc cagatgccgc cgcccacgat agcaacgctc
     9901 gctcctcggc tcatcccgtc ctccgatgta ccggagctaa ccgccgactc tcctcagttc
     9961 aggagcttct gtgcgtcggt gtaggagagg tcgtgggcct cggcgaccgg ctcggagacg
    10021 aggtctcccc tgagtgtaga gagtcctttg gccagggact catcttcggt cgccgcaccc
    10081 tcgatgcctt tgtcggctat tttgatcaaa tagggcaaag tcgcgctggt cagggccagg
    10141 gtcgaggtcc tcgccactgc ccccggtatg ttggcgacgc agtagtgcac gacgccctcc
    10201 tcgacgtacg tgggatctga gtgcgtcgtc ggcctggcag tctccacgca cccgccctgg
    10261 tcgatggcga cgtcggcgac cacgctccca ggacgcatgc tcgcgaccat ctctcgcgtg
    10321 acgagcttgg gcgccttcgc gccgtgcacg agcacggccc ctatgaccac gtcggcctgc
    10381 ctgacatacg cggcggtcct ggcgcggttg ggtatcacga ggtcggcgcg tccctcgaag
    10441 atatccgaga ggtaggctag ccgcttggga ctgatgtcga gcacgctcac cagcgcccgc
    10501 atgccgaggg cgatcttggc ggcctcggtg ccgaccactc caccaccgat gatggtgacc
    10561 ttggcggcag gggttcccgg caccccaccg agcaacaacc ccgctccgcc ctgcgggctc
    10621 tcgaggtggt gcgccgcagc ttgtacggac atcctcccgg ccacctcgct catgggagta
    10681 aggagcggca gagatccatc ggaggcctcg accgtctcgt aggcgatgga gttgatcctg
    10741 cgctccatca agaactccgt gagccctttg tccgccgcca ggtgcaaata cgtgaagagc
    10801 tgctggccct ccctgaagcg gtcgtactcc tcggggactg gctccttgac cttgacgatc
    10861 agatcagaag actcgaagac ctcatcgggc ccgtcgacga gacgcgctcc cgcctcgtcg
    10921 tactcctcgt cggagaaacc cgagccttcg ccggcaccgg cctggacata aacctcgttg
    10981 ccgtaatgaa ccaactcggc aacgccgtca ggctgcatgg agacacggcc ttccatgtcc
    11041 ttgatctctt tcggtacccc aattactgtc gccatagcct tcctctccct cgttgcgccc
    11101 acgctcttcc taccttatag caaggctact ataaacgtgc aaggatgctg gcagggacag
    11161 ccttccatcg tggtgcgcca gggctgctaa tcaggccgcc ctgccgaggt cgtgccggga
    11221 cgcagccaca gtgatgcg
//
LOCUS       DATMVF010000020        14806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_369816, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000020 DATMVF010000000
VERSION     DATMVF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14806
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369816"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      155..232
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            225..485
                     /locus_tag="VN178_00665"
     CDS             225..485
                     /locus_tag="VN178_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79502.1"
                     /translation="MPNRQERDQGWNHQEWEPSRALTSALQELPLLAQTSRNEDLANL
                     EPHLEEALQTLERLEQRRGLSDREKIREGALRMLLTSIEEVK"
     gene            complement(524..919)
                     /locus_tag="VN178_00670"
     CDS             complement(524..919)
                     /locus_tag="VN178_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79503.1"
                     /translation="MDGTTTTVVGIIGDDPLVSRILGLLLEGAGYEARLVDRFAALED
                     PSVALAGVDLVLFLPLLGDEHKGELLGVVKGDPATADLPALSLSTDMKAQLDERSDSV
                     LPWPWSTEALVQAIEQAVHTPGGDEAPRR"
     gene            complement(1167..1391)
                     /locus_tag="VN178_00675"
     CDS             complement(1167..1391)
                     /locus_tag="VN178_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79504.1"
                     /translation="MDEEILPKIDYYLDESDPDIVILRRQDDTFVAAFSAQGVTKEGI
                     LEAAKEDYRKLIELHSGSRNPREEDRRSTA"
     regulatory      complement(1602..1688)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            1977..3683
                     /locus_tag="VN178_00680"
     CDS             1977..3683
                     /locus_tag="VN178_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWS79505.1"
                     /translation="MARKEEEQLRFQAKLLEAVGQSIIATDLEGKVLYWNSAAEELYG
                     WSSEEALGRRLRDLTLSEELLDQAEVVASHLRAGRTWSGETLLRRKDGSYVPVLGTAT
                     PFFDDRGHPAGMIGVSTDISERKALEAELERRASHDLLTGLYNRRVFVDRLGQALLRT
                     RRRKNSLKVGVLFMDLDRFKTINDYLGHEAGDRLLVTVAERFKQRLRPEDVIARFGGD
                     EFAVLLETVEDPSEVIRVAQRIVESLREPFTVDDHQVGVSTSVGIALGTAHANDDPEG
                     MLREADVAMYRAKEQGPGRYALFDPAMQTRAQERIELEAELRRALEQGEFVLFYQPEV
                     SLHNGSMVGFEALLRWQHPERGLLKPSAFVPLAEETDLITPIGRWVLEEACRQAKRWE
                     VEHLPAPPATMEVNLSARQLARRGLAQTVEEALRRANLAAHSLALDITETVLIGASEH
                     NAQALEALKKMGIRLYLDDFGTGYSSLSYLKRLPVDRVKVDRTFVKGLGGNATDTALV
                     RMIIELCHTLGIEVLAEGVETSDQAALLKDMGCDLGQGYYFARPLRSEELAEQLPEAF
                     LP"
     gene            4057..4215
                     /locus_tag="VN178_00685"
     CDS             4057..4215
                     /locus_tag="VN178_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79506.1"
                     /translation="MKSTDIELMISVEEQELMERLELDSWPEILRLVGQCSILVGIVG
                     IIAGLIAA"
     gene            complement(4321..8745)
                     /locus_tag="VN178_00690"
     CDS             complement(4321..8745)
                     /locus_tag="VN178_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWS79507.1"
                     /translation="MYQRTRGRLSKLRSNLLLLLVVCGMLLAAPAIVMALITDLGNDT
                     LPTIQNEGAKVANPGTDSREDASPAPAIQNEEANIGAPSADALPAQAIQIEESKTAAG
                     KNITLTGSNWQPGESVHVKLTDDQGKTWSGNVGVTADAGGRIQVRFQLPAGFSATYEV
                     TAMGEQSGVATTSFAGGNVSFSGTLPAPARWTVNYRTHGGGNATHDTSCAAGGTDGSR
                     TIFPDTMGTASVAISDKGSMRLGTVTVPADSTLVFDHWTDQNGNTVANDACLSGDPGG
                     PNGNITDLIAHFDPTKQTPEDTTKQAPEAQPQPIVSTNEDTAKTITLSGTDEDGDNVL
                     FYIATGPSHGTLGPIGALTCAGTVPRTCSANVTYTPDDDYNGFDIFSFKADDGTEGSY
                     PAPVSMLVDPVNDTPVATDETKTMSEDGGPLSIDFGGLLSDVETSDADLTYGITDPAK
                     GSLSGAGSTREFTPAADFNGTVKIDYAPTDRGDPDECRAANPPCAGAAIGAKKSVTVT
                     VEAVNDAPSFTKGPDQTTNEDAGAQTITDWATAISAGPNETGQRVTFEATNDNNDLFA
                     SGGQPSVAPDGTLTYTPAENANGTARVVVKLRDDGDTANGGADTSAEQSFTIAVDSAN
                     DAPGFRKGDNQTVNEDSGAQVVNGWATDISAGSADESGQRLTFEATSDTNLFTAGGQP
                     EIDASGKLTYEAAPNANGSATVAVKLRDDGGTLNGGANTSAEQSFTINVTAANDTPIA
                     RRDTLATVEDRKLVFPSSDLVGNDYEGADNESSQTLAVTEVYEGAAGTVSLGSNGNIT
                     FEPEADFGGYAAFRYLVCDDGSPSKCSVQRATVNVPVSPVNDAPAAGNQSVTTDEDAA
                     VAVTLTAPDAEGDAVGYIIVSAPRHGTLNGSGANLIYTPEADYRGPDSFTFKASDGIA
                     DSDPATVSIAVDSVNDAPVATSDTMSTNEDTPLVFAAGKLLGNDTPGTANESDQTLTV
                     TAVDLPVNGQVSLGTNGDITFTPDTGFDGQASFEYTVCDNGSPSECSDMVAIVDVKAS
                     PVNDAPGFTAGADQTVTEDSGPHSIPGWASGISAGPADESDQELTFEVTNNTNTGLFS
                     TQPSVAADGTLSYALATDQNGSSDVTVRLKDNGGLANGGADTSAEQTFTIDVTALNDA
                     PIVSNMQGDGAANEGDVKTYTFDIADVDSGAFSASVVCGGPGKGELVPGSVRISGTNG
                     RFDCEFLDGLISSTPVDISVRVSDGDRLSNVESKSVGVSNVAPALTGISSSSQNALAG
                     TLNPVTFTGTATDVSPIDLAAGFRWRWAIDGGAYGPFGAVNANTFEVGDANNQHSFST
                     CGTHTVEAQAIDKDGGVSAESSQAAQSVSVYDGVFSPPLVDGTTNMVQKGQVIPVEIS
                     VGCGTTDLTNLTPHIQLLGGNVSPEAESGRTTLTTTPVLAADTTQTMRPVDGGYIYNL
                     QVPNNAAANQKFTILVDPFGATADHAATGMYVVLKVGK"
     gene            complement(9008..10750)
                     /locus_tag="VN178_00695"
     CDS             complement(9008..10750)
                     /locus_tag="VN178_00695"
                     /inference="COORDINATES: protein motif:HMM:NF011539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HWS79508.1"
                     /translation="MEERTQGYRLVTTAEDLGAVAKTLQSAETIGVDIETTALSPRDG
                     GVRLLQLATPEETFVIDIFEVGDLSPLTGVLEGGPVKVGHNLKFDYQFLSALYGVSLS
                     PLFDTMLAAQVLDGGNYGASYSLEAVVERYLDESLDKSEQRSDWSGELSEAQLEYAAR
                     DARVLLPLRERLAASLEEEELGRISRIEFAAVAAIAEMELAGVKLDVARWKELEVRVR
                     ERRDRAADHLESFFPPPEGILPLEGLGPRLNLNSPKQITDAFRTLGIELPDTKVWTLL
                     KVDHPAAKALLEYRELQKKLGTYLETYPGFISPKTGRIHANFLQCRVPTGRLACTAPN
                     IQQIPHEDEFRRCFVAEDGNTLVIADYSQIELRILAEVSEDPAFVGAFQRGMDLHRLT
                     AATMYNVSMDEVTKDQRTAAKRINFGLMYGRGARSLSAQLGTDEERGRRLIDEYFANY
                     PKVQRFLQRTANRAVRDRTLRTLAGRVRKFGNDPVADDRGAMRREAMNFPIQGAASDI
                     AKLALVYVREEVEGLDARLINSIHDEFVIECAEEQATEVSEKTQAAMVRAGEDILEKV
                     PVQVEVEISREWRK"
     gene            10825..11931
                     /locus_tag="VN178_00700"
     CDS             10825..11931
                     /locus_tag="VN178_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HWS79509.1"
                     /translation="MGGSFKIGSAFGIDVKVHWSFFLLLAFFGYLAFGDSGSFVDALV
                     TVGVVVVLFFCVLLHEFGHSLVAMRLGSEVQDITLLPIGGLARMKSLPERPIDEVKVA
                     VAGPLVNVVLAPIFFGLALLFGGNLSVPTDILSGVGSLGQLFATLGMINVALVVFNMI
                     PAFPMDGGRVLRGLLATRLGPLRATNISSTVGQAFAVLFILLGFFSNNFLLAIVGFFV
                     FLGASGEAGMVRQREQMRGLTVSDVMGTKGRIETVTPYHNFGQILDSVIHGYQEDFPV
                     LDEEGDLVGIITRNEIMAAAHSPGRFATVRDLMKTEFPTISPDADLFTDGNRILQESG
                     FRALPVVKDGNLVGMLTVDDVGQAALLRDLRERR"
     gene            12036..12638
                     /locus_tag="VN178_00705"
     CDS             12036..12638
                     /locus_tag="VN178_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWS79510.1"
                     /translation="MKTVVEEIEEHIARVGTHPVYGYVHCLRVFALAEELAALEGISY
                     DEEILRAAALLHDVGLYKAYALREPSDHAKRSAVVAGRILKDWDFPPQASQAVIEAIE
                     HHPPGAPAGTASETVLLKDAVGLDYLGAIGISRMLAMVGTEEDVPDIRAAIWNAESLR
                     RRIPDLLVLRSSKDLAAQRIREMDAFVEDLRYSTAKLKLL"
     gene            12866..13348
                     /locus_tag="VN178_00710"
     CDS             12866..13348
                     /locus_tag="VN178_00710"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HWS79511.1"
                     /translation="MNGLLFALTFVAALGCGVIAGVFFAFSAFVMSALARLPAQQGIA
                     AMQAVNVAAITPAFMAALFGTALACGTLIVSSFLVWGEPFAGYLLTGGALYLVGTIVT
                     TVAYHVPRNEALARIEPHGAGAEDHWSRYLRGWTAWNHLRFAAALAAAATLTIALRIG
                     "
     gene            13530..13814
                     /locus_tag="VN178_00715"
     CDS             13530..13814
                     /locus_tag="VN178_00715"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HWS79512.1"
                     /translation="MHFPQPRSGRFWLVALACALYIGGCFLLTVFVNVPLNEELARVA
                     EGASSAELAGARAAYEGPWNFWNGVRTVFSSLAFLALIGACLLCEDRGAS"
     gene            complement(13811..>14806)
                     /locus_tag="VN178_00720"
     CDS             complement(13811..>14806)
                     /locus_tag="VN178_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS79513.1"
                     /translation="GEPALAKATFERWTNRVPEDADMLINAGLTLFDAADYAEALEFF
                     EKAIEAAADDLQKCGARTFRANALDMLGRYAEAVGAYEEVIAAAPEWWEAHANLGICH
                     ARNGDLEKAEAAFRQGLEDCPGSPEIRDELAAHLLALRKNLPEALRLSEEAVALGRDE
                     IRHLITLGEARLALEDEDGAAAAYDAVLALDPENPEAHLELGILSERKGEMGRAEEHF
                     VESLKVDPANPRALYSYASLYYTADDLEVAEEILERAIAADARYSPALSALASIQARR
                     GEYGDALDYIEKAVDAGERDVEHFRSALEFAPLHADPRFRTLLSRMVGDRSNGQG"
BASE COUNT         2505 a         4577 c         4762 g         2962 t
ORIGIN      
        1 aggtcggtca gttcctcgct ggcggtcctg ggccctgtga agcggggcta tgctgaacct
       61 cgtctgacgg agttcttgga tgccggttag ccaagcacga aacggccgcc cggcatacat
      121 atcgtgtctg cgatggatta caatgctcga atacgttcga caagggcaaa cccgtcgcaa
      181 ggcgggggcg caaagcctac ggacctctgg aggaggtagc cgggttgccg aaccgccaag
      241 agcgagacca aggatggaac caccaagagt gggagccaag cagagccctg actagcgccc
      301 tccaggagct gccgctcctg gcccagacgt cccgaaacga ggacctggcg aacctcgaac
      361 cccacctgga ggaggcgctt cagacgctgg aacgcctcga gcagagaagg ggtctttcgg
      421 atagggaaaa gattcgagaa ggggccctca ggatgctgct gacgtcgatc gaagaggtca
      481 agtagaagtc agtacgcaac ggaagggcgg cacacgtacg ccgctaccta cgcggcgctt
      541 cgtctccgcc aggcgtatgc acggcttgct cgatcgcctg cacgagagct tccgtggacc
      601 acggccaggg caaaaccgag tccgaccgct cgtccagctg cgctttcatg tctgtggaga
      661 ggctgagggc cggcaagtca gcggtcgccg ggtcgccttt cacgacgccc agaagctcac
      721 ccttgtgttc gtcgccgagt aatggcagga agagcaccag gtctacgccc gccagcgcca
      781 cgctcggatc ctccaaggcg gcgaacctgt ccacgaggcg cgcctcgtag ccagctccct
      841 cgagcaacag ccccaagatc cgggagacca gcggatcatc gccgatgatc cctaccaccg
      901 tcgtcgtagt accatccacc ggcgctcctc tcttactcgg agaacgggga aagaatgagt
      961 attcacccaa ccttgtagaa ggtagatttc tcgctcgtac accgtataca cacccaacat
     1021 tcatcgcccc tggccagggt gtaggcggga cagagcgaca cagggctcct ttccttccga
     1081 gagtatcctg atctccttcc tctccgtagc ccttcagcag ccaagcgcct acgagttctg
     1141 ctctcgatag tgcgctccga gccattctag gcggtgcttc gccggtcttc ttctcgaggg
     1201 ttcctggagc ctgagtgtaa ctctatcaac ttcctgtagt cttcctttgc ggcctccaag
     1261 ataccctcct tcgtaacccc ctgagcgctg aaggcggcca caaacgtgtc gtcttggcgg
     1321 cgcaggatga ctatgtccgg gtccgactca tcaagatagt agtcgatctt ggggagtatc
     1381 tcctcatcca tgctcactac tccttggaac atcaccttgg caagtgaggc caaaaggctg
     1441 cgtggtctct agctcaattc cttcgagcgc gtgtaaggcg tgttctcgga aggacacata
     1501 gccccggtcc aacacccatg cggcctatgc tgtctttggt gacttaacgc caccaaaagg
     1561 actaacaaga cgcgtttcac aacaggctac ctccttgctg ttcgtgcggt agccggttgc
     1621 gccactggct ccactcgtcc caaaaggtgc ccaactgcgg gacgggcttc tcggcctcta
     1681 ccttctttcc ctcgcaaccg gtagcataaa gccacggccg cagacaagca acccccatat
     1741 cgcatcctcc gggaggagta gcccttaccg cttgaagtgc ctaggaggtg aaagttgcgc
     1801 ggtagagccc cgagcatgta tgctgcgtgt actgcaaaac ccagctgctg atagaggctg
     1861 agaagctcac tcccatacct gggtgcctcg gatgagtgga gccagtggcg caaccggcta
     1921 ctaccgcaaa cggtgctagc cggttttgtt ttgcaggttt gatggaggtg catatcttgg
     1981 cacgtaaaga ggaggagcag ctacgtttcc aggccaagct gctcgaggcc gtggggcaat
     2041 ccatcatcgc caccgacctc gaagggaagg tcctctactg gaacagtgcc gcagaggaac
     2101 tctacggctg gtcctcggag gaggcgctgg gacgcaggct gagggacctg acgctctctg
     2161 aggagctgct ggatcaggca gaggttgtcg cttcccacct acgggcggga agaacctggt
     2221 cgggcgagac gcttctcagg cgcaaggacg gctcctacgt gcccgtgttg ggtaccgcca
     2281 cacccttctt cgacgaccga ggacaccccg caggcatgat cggcgtctcc accgacatct
     2341 ccgagcgcaa ggccctcgaa gcggaactcg aacgccgggc ttcccatgat ctcctcacgg
     2401 gcctgtacaa tcgtcgcgtc ttcgtcgatc gcctcggaca ggccttgctg cgcacgaggc
     2461 gtcggaagaa cagcctcaag gtgggagtgc tgttcatgga cctggaccgc ttcaagacca
     2521 taaacgacta cctggggcac gaggccggcg acaggctcct cgtcacggtc gccgagcgtt
     2581 tcaagcagcg gttgcggccc gaagacgtca ttgcacgctt cgggggagac gagttcgccg
     2641 tgctgctgga gaccgtggag gacccctccg aggtgatccg ggtggcgcag aggatcgtcg
     2701 agagtcttcg ggaacctttc accgtggacg accatcaggt gggcgtcagc accagcgtcg
     2761 gcatcgcttt gggcactgcc cacgccaacg acgaccccga agggatgcta cgcgaggcgg
     2821 acgttgcgat gtaccgagcc aaggagcagg gccccggccg ctacgcgctg ttcgacccgg
     2881 ccatgcagac tcgcgcgcag gaacgtatcg agctagaggc cgaactcaga cgagccctag
     2941 agcaagggga gttcgtactc ttctatcaac ccgaggtctc cttgcacaac gggagcatgg
     3001 tcggcttcga agccctgctg cgctggcaac atcccgaaag gggactactc aaaccgtcgg
     3061 ccttcgtgcc acttgccgag gagacggatc tcatcacgcc tatcggcagg tgggtgctgg
     3121 aggaggcttg ccggcaggcg aagcggtggg aagtagagca tcttcctgcc cctcccgcta
     3181 cgatggaggt caacctctcc gcaaggcagc tagcacgccg agggctggca cagaccgtcg
     3241 aagaagcact gaggcgagca aacctggctg cccactcctt ggcgctggac atcaccgaga
     3301 ccgtcctgat aggagcctcg gagcacaacg cccaagcact tgaagccctg aagaagatgg
     3361 gcatccggtt gtacctggac gacttcggca ccggctattc ctcactctcg tacctaaagc
     3421 gcttgcctgt ggaccgcgtg aaggtggata ggacgttcgt aaaagggctc ggagggaacg
     3481 cgacggacac agcattggtg cgaatgatca tagaactgtg ccacaccttg ggcattgagg
     3541 tgctggccga gggggtcgaa acctccgacc aagcagcctt gctgaaggac atgggatgcg
     3601 atttgggaca ggggtactac ttcgcacggc cgctgcggag tgaagagcta gcggagcagc
     3661 tgcccgaagc gtttctaccg tagggtcgcc gcaatgatct tagggctagc cggaacggtc
     3721 atgttcgatt ggctaagtga gctttcggca ctcggcgctg gtcggcaact ggctgtcctc
     3781 gaggcagacg ccgcacaccg gctctctgac tcggagtatg tggccttgcg gctcaggctt
     3841 gaagcgcaac agaggcggcg ctggaggagg cttggggtag ggtgattctc agcccgttct
     3901 catactacgc tgttagatat tctagtcatt agagctcgac cggatgagga aggggaccat
     3961 ataggcgccg gtacagagtc aatcgagtgg agcagaccct ggagcagcta gcccaccact
     4021 ttactctccg gtcccaccta cacacaggag atgtatatga agtccacaga cattgagctt
     4081 atgatctcgg tggaagaaca ggaactcatg gaaaggctgg agctagactc atggccggag
     4141 atccttaggc tagtgggcca atgctcgatt ctcgttggga ttgtcggtat catcgcgggg
     4201 ctcatcgcag catagcggta ggctaacgca gcacgctagg cccccagaag agaagggccg
     4261 gggtgttgtg caccggccct ttcgtgctcg gactatccga tcagcccgtc ttttcatctc
     4321 ctacttgccg actttgagga cgacgtacat gccggtggct gcgtggtctg cggtggcccc
     4381 gaaggggtcg accaggatgg tgaacttctg gttcgccgcg gcgttgttag gcacctggag
     4441 gttgtagatg tagcccccat cgaccgggcg catagtctga gtggtatcgg ccgccaggac
     4501 cggggtagtc gtcagggtcg tcctcccgga ttcagcctcg ggcgagacgt tgccgccgag
     4561 aagctggatg tggggagtca ggttggtcag gtcggtggtg ccgcacccga cggagatctc
     4621 caccgggatg acctggccct tctgcaccat gttggtggtg ccgtcaacaa gcggcggact
     4681 gaagaccccg tcgtacacgc tcacggactg cgcggcctgg gatgactcgg ccgatacgcc
     4741 gccgtccttg tcgatggcct gcgcttcgac ggtgtgggtg ccgcaggtgg agaaggagtg
     4801 ctggttgttg gcgtctccga cctcgaaggt gttggcgtta acggcgccga acgggccgta
     4861 agcgcctccg tcgatcgccc accgccacct gaatccggcg gcgaggtcga tcggcgacac
     4921 atcggtcgcc gtgccggtaa aggtcacggg gttgagcgtt cctgccaggg cgttctgcga
     4981 agagctgctg atgcccgtta gcgcgggagc gacgttgctg acgccaacgc tcttggactc
     5041 aacgttgctt agcctatcac cgtcgctgac ccgtacgctg atgtcgaccg gggtagatga
     5101 gatcaggccg tcgaggaact cgcaatcgaa ccggccgttg gtgccgctga tccggacgct
     5161 cccggggacg agctcaccct tgcctggccc gccgcagacg actgaggcag agaaggcgcc
     5221 gctatccacg tcggcgatgt cgaaggtgta ggtcttgacg tcgccctcgt tggcggcgcc
     5281 atcaccttgc atattgctga ctatcggcgc gtcgtttaag gcggttacgt cgatggtgaa
     5341 cgtctgctcg gcgctggtgt ccgctccgcc gttggcgagg ccgccgttgt ccttgagcct
     5401 cacggtaacg tctgagctgc cgttctggtc ggtggcgagc gcgtagctga gcgtaccgtc
     5461 ggctgcgacg gaaggctgag tagagaacag cccggtgttg gtgttgttgg tgacctcgaa
     5521 ggtcaattcc tgatctgact cgtcggcggg accagcggag atgccgctgg cccaaccggg
     5581 gatgctgtgt ggtccagagt cctcagtcac agtctggtcg gcccccgccg tgaagccggg
     5641 agcgtcgttg accgggctcg ccttgacgtc cactatcgct accatgtcgg agcactcgct
     5701 cgggctgccg ttgtcgcata cggtgtactc gaagctagcc tgtccgtcga agccggtatc
     5761 cggcgtgaag gtgatgtcgc cgttggtgcc aagagagacc tggccattga cgggtaggtc
     5821 tacggcggtg accgtcaggg tttggtcgct ctcgttggcg gttcccggag tgtcgttgcc
     5881 aagtagctta ccggccgcga agaccagagg agtatcctca ttggtcgaca tggtgtcgct
     5941 ggtggccaca ggtgcgtcgt ttacggagtc gaccgcgatg ctgaccgttg ctggatcgct
     6001 gtcggcgatc ccgtcggagg ccttgaaggt gaagctgtca ggacctctgt agtcagcctc
     6061 cggtgtgtag atgaggttgg cgcccgagcc gttgagggtg ccgtgccgcg gagcactcac
     6121 gatgatgtag cccacggcgt cgccctctgc atcgggggca gtgagggtaa ctgctacggc
     6181 cgcgtcctca tcggtggtga ccgactggtt gcctgctgcc ggtgcgtcgt ttacggggct
     6241 gaccggcacg ttgaccgtcg ccctctgcac ggagcacttg ctcgggctac cgtcgtcgca
     6301 caccaggtat ctgaaggcgg cgtagccacc gaagtcggcc tccggttcga aggtgatgtt
     6361 gccgttagaa ccgaggctta cagtgccggc ggcgccctca tagacctccg tgacggccag
     6421 ggtctggcta gactcgttgt ccgcgccctc ataatcgttg cccacgaggt cgctagacgg
     6481 gaagaccagt ttcctgtcct cgacggtcgc cagggtgtcc ctgcgggcga tgggcgtgtc
     6541 gtttgcagcg gtgacgttga tggtgaagct ctgctcggca ctggtgttcg ctccgccgtt
     6601 cagcgtgcca ccgtcgtcgc ggagcttcac ggcgacagtt gcgctgccgt tggcattggg
     6661 cgcggcctcg taggtgagct tgccgctagc gtcgatctcg ggctgccccc cggcggtgaa
     6721 caggttggtg tcactggtgg cctcgaaggt caaccgttgg ccactctcgt cggcgctgcc
     6781 agcagagatg tcggtagccc aaccgttgac cacctgcgca ccggagtcct cgtttaccgt
     6841 ctggttgtcg cccttccgga agccaggcgc gtcgtttgca gagtcgacag cgatggtgaa
     6901 gctctgctcg gcactggtgt ccgctccgcc attggccgtg tcgccgtcgt cgcggagctt
     6961 cacaacaacc ctggccgtgc cgttggcatt ttcggcgggg gtgtaggtaa gcgtcccgtc
     7021 gggggcgacg gaaggctgtc ctcccgaggc gaacaggtcg ttgttgtcgt tggtggcctc
     7081 gaaggtcacc cgctggccgg tctcgttggg accggcagag atagcggtgg cccaatcggt
     7141 aatggtctgc gctccggcat cctcattcgt ggtctgatcc ggacccttgg tgaagctggg
     7201 ggcgtcgttt acggcttcga ccgtaacggt cacggacttc ttggcgccga tcgcggcccc
     7261 ggcgcaagga gggttggcgg cccggcattc gtccggatcg ccgcggtcgg tgggcgcata
     7321 gtcgatcttc acggtgccgt tgaagtcagc agccggggta aactcgcgag tggagcctgc
     7381 tccgctgagc gaacccttag cgggatcggt gatgccatag gtgaggtcgg catcggaagt
     7441 ctccacgtcg gagagcagcc caccgaagtc tatggagagc ggaccgccgt cctcgctcat
     7501 cgtcttggtt tcgtcggtgg ccaccggggt gtcgttgacc ggatcgacca acatggacac
     7561 cggagcaggg tagctgcctt cggtgccgtc gtcggctttg aagctgaaga tatcgaagcc
     7621 gttgtagtcg tcgtccggcg tgtaggtgac gttcgcggag caggtcctcg gcaccgtacc
     7681 cgcgcacgtc agcgccccga tcggcccgag ggtcccgtgg ctcgggccgg tggcgatgta
     7741 gaagagcacg ttatcgccgt cctcgtcggt gccgctcaag gtgatcgtct tggcggtgtc
     7801 ctcgttggtg ctcacgatcg gctggggttg cgcctccgga gcctgtttcg ttgtatcctc
     7861 cggagtctgc ttcgtcggat cgaagtgcgc gatgaggtcg gtgatgttgc cattcgggcc
     7921 tcccgggtcc ccggagagac acgcgtcgtt ggcaaccgtg ttgccgttct ggtcggtcca
     7981 atggtcgaat acgagcgtcg agtcggcggg cacggtcacg gtcccaagcc tcatagaccc
     8041 tttatcggag atggctacgg acgctgtgcc catggtatcg gggaagatgg tcctggatcc
     8101 gtcagtgccc cccgcggcgc aagaagtgtc gtgggtagcg ttcccaccgc catgcgtcct
     8161 gtagttgacg gtccacctcg caggagcggg taaagtaccg ctgaacgaca cgttgccgcc
     8221 ggcgaaggac gtcgtcgcca cgccggactg ctcacccatg gcggttacct cgtaggttgc
     8281 cgagaacccg gcggggagct ggaaccggac ctgtatccga ccacccgcat cggccgtcac
     8341 gcctacgttg ccgctccagg tcttgccctg atcatccgtt agcttgacat ggacggactc
     8401 gcctggctgc cagttggatc cagtaagggt tatgttcttg cccgccgcag tcttcgactc
     8461 ctcgatctgg atcgcctgcg cgggcaatgc gtcagcgctc ggggcgccta tgttagcttc
     8521 ctcgttctgg atcgcgggcg cgggcgaagc gtcctcgcgc gaatcggtgc cggggttggc
     8581 tactttcgcc ccctcgttct ggatcgtggg caatgtatcg ttgcccagat cggttatgag
     8641 tgccatcact atcgccggtg cggcgagcaa catcccacac acaacgagta ggagcagaag
     8701 gttgctcctc agcttcgaca gccttcccct cgtcctttga tacatacgct acccacctgt
     8761 tggcgaagcc tcatatttgt ttgttactgg taaggttctc acagttcgct tcgctacatc
     8821 aagaacagac tatcgggtag ggagtacagt gacgtctgcc aggtggccat ttctggtgaa
     8881 atccgtgcgt cgaggtatgc caagggttat tcgcacaacg caaacagtcg cccaccggcg
     8941 ggggaagggg cctaagagct accccgagct tgtgctgagt agccggttgt tccggcgggc
     9001 ggagccctca cttcctccac tcgcgggaga tttcaacctc gacctggacg ggcaccttct
     9061 cgagaatatc ctcccccgcc ctgaccatcg ccgcctgcgt cttctcggag acctcggtgg
     9121 cctgttcctc ggcgcactcg atgacgaact cgtcgtggat ggagtttatg agacgggcgt
     9181 cgagaccttc gacctcctcg cggacgtaga ccagggccag cttggctatg tcgctggcgg
     9241 ccccttgaat cggaaagttc atcgcctcgc ggcgcatggc gcccctgtcg tctgccacgg
     9301 ggtcgttgcc gaacttgcgc acccggcctg cgagcgttcg gagtgtgcgg tcccgcacgg
     9361 cccggttggc cgttcgttgc aagaagcgtt gcactttggg gtagttggcg aagtactcgt
     9421 ctatgagtcg gcgcccacgt tcctcgtccg tgcctagctg ggcggagaga ctgcgggcgc
     9481 cgcgtccgta catcaggccg aagtttatgc gcttggccgc cgtgcgctgg tccttggtga
     9541 cctcatccat ggacacgttg tacatcgtgg ccgccgtcag gcggtggagg tccatgcctc
     9601 tctggaaagc cccgacgaag gcgggatctt ctgagacctc ggcgaggatc ctcagctcta
     9661 tctgcgagta gtcggctatg acgagcgtgt tgccgtcttc ggccacaaag cagcggcgga
     9721 actcgtcctc gtgcggaatc tgctggatat tcggagccgt acaggcgaga cgcccggtcg
     9781 gaacgcggca ctgcaggaag ttagcgtgaa tgcggccggt cttcgggctt ataaagccgg
     9841 ggtaggtttc gaggtaggta ccgagtttct tctgtagttc ccgatactcg agcagtgcct
     9901 tcgccgccgg gtggtccacc ttgaggaggg tccagacctt cgtgtcgggg agctctatcc
     9961 ccagggtccg gaaggcgtcc gtgatctgct tggggctgtt gaggttgagt cgcggtccga
    10021 ggccctcgag cggcaatatc ccttcgggcg gcgggaagaa ggactccagg tggtcggcgg
    10081 cccggtcgcg tcgctcgcgc accctcacct ctagctcctt ccagcgggct acgtcgagct
    10141 tgacgccggc gagttccatc tcggctatcg ccgcgacggc cgcgaactct attcgagaga
    10201 ttcgtccgag ctcttcctct tctagcgatg ccgcaagtcg ttcgcgcaga ggcagcaaga
    10261 cccttgcgtc ccgtgcggcg tactcgagct gtgcttcgct caactctcca gaccagtcgc
    10321 tcctctgctc ggacttgtcg agcgactcgt cgaggtaacg ctccaccacg gcttccagcg
    10381 agtaggatgc cccgtagttg ccgccatcga ggacctgggc agcgagcatc gtgtcgaaga
    10441 ggggcgagag cgagacaccg tacagggcac taaggaactg gtaatcgaac ttcaggttgt
    10501 gccccacctt gaccggcccg ccttcgagca cccctgtgag tggtgagagg tcgccgacct
    10561 cgaagatatc gattacgaac gtctcctccg gggtggcgag ctggagcagg cgcacccctc
    10621 cgtcgcgggg ggagagggcc gtggtctcga tgtccacgcc gatggtttcg gcgctttgca
    10681 gggtcttcgc gacggcgcca aggtcttccg cggtcgtaac gagacggtag ccttgggtac
    10741 gttcctccac ccctcgattc tagcaggcag agggggtttc ggatcacacc gcccgccggt
    10801 gcgtggggta aactcaacgc aataatgggt ggatctttca agataggcag tgcgttcggt
    10861 atagacgtga aggttcactg gagtttcttc ctgctgctgg ccttcttcgg ctatctcgct
    10921 ttcggagatt cgggcagctt cgtggacgcg ctcgtcaccg tcggcgttgt ggtcgtactg
    10981 ttcttctgcg tcttgcttca cgagtttggc cactcgctcg tggccatgcg tctcgggagc
    11041 gaggttcagg acatcacgct cctgcccatc ggcgggctgg ccaggatgaa gtcacttcct
    11101 gagagaccca tcgacgaggt gaaggtcgcc gtagccggtc cgctcgtcaa cgtcgtgctc
    11161 gcccccatat tcttcggact cgccctgctc ttcgggggca acctctcggt tccgacggac
    11221 atactctccg gggtagggtc cctgggccag ctcttcgcca cgctaggcat gatcaacgtc
    11281 gccctcgtgg tcttcaatat gatcccggcc ttcccgatgg atggtggccg tgtgctgcgc
    11341 ggcctgctcg ccacgcgcct cggacccttg cgcgccacga atatctcctc gaccgtaggg
    11401 caggccttcg ccgtcctctt catcctcttg ggcttcttta gcaacaactt cctgctcgcc
    11461 atcgtcggct tcttcgtctt tctcggcgcg agcggcgagg cagggatggt ccgccagagg
    11521 gagcagatgc gcgggctcac ggtctctgac gtaatgggta ccaagggccg aatcgagacg
    11581 gtaacgccct accacaactt cggccaaatc ctcgactccg ttatccacgg ctaccaggag
    11641 gatttccccg tcctcgacga ggaaggagat ctggtcggca tcatcacccg caacgagatc
    11701 atggccgcag cccactcccc cggccgtttt gccaccgtcc gcgacctgat gaagacagag
    11761 tttcctacca tctcacccga cgccgacctc tttacagacg gcaaccgtat ccttcaggag
    11821 agcggcttca gggccctgcc cgtcgtcaag gatggcaacc tggtggggat gttgaccgta
    11881 gatgatgttg gccaggccgc cctgctccgc gatcttcgag aaaggcggta agcgcagggg
    11941 cgctccgtgc gtagggtgct tcgcgaagac gtaggggcgg gtttcaaacc cgtccctact
    12001 cgcaccgtcg ggacctattc gctagactct ctgccatgaa aacggtggta gaggagatcg
    12061 aagagcacat cgccagggtt gggacgcacc ctgtttacgg gtacgtccac tgcctgaggg
    12121 tcttcgccct ggctgaggag ctagctgccc tggagggaat ctcctacgac gaggagattc
    12181 tgcgcgcggc cgccctgctg cacgatgtcg gtctctacaa ggcctacgcc ttgcgggagc
    12241 cgtccgacca cgccaagcgc tcggcggtgg tggccggccg tatcctcaag gactgggatt
    12301 ttccccctca ggcgagccag gcggtcatag aggcgatcga acaccaccca ccaggggcgc
    12361 ccgccggcac tgcctctgag accgttctcc tcaaagacgc ggtcggcctc gactacctgg
    12421 gcgccatcgg tatctcccgc atgctggcca tggtcggcac ggaagaagac gtgccggata
    12481 taagggcggc gatctggaac gcggagagcc tgcggcggag gatccccgac ctcctcgtcc
    12541 tgaggagcag caaggatctg gccgcccagc gtatccgtga gatggatgct ttcgtcgaag
    12601 acctgagata ctctacggcg aagctgaagc tgctgtagac ggccccgaag gggcgcactc
    12661 ttaccggcta cgcccccggc atctcgcacg aaggcacgcc tggtcagctc ttgcatccgg
    12721 cccacacacc gactcacagg ccatccgcgt gtcaatctac actgtgtaaa gcaaggagaa
    12781 aggagccgag tatggaggcc gagaacgggc acgaaagccg cctcttccag cgtgtgggct
    12841 gggatccgct aggacgtgtc ggccgatgaa cggtcttctg ttcgcgctga cgttcgttgc
    12901 agccctcgga tgtggtgtga tagccggggt cttcttcgcc ttctcggctt tcgtgatgag
    12961 cgctctcgcc cggctgccgg cccagcaggg catcgccgcc atgcaggcag tcaatgtggc
    13021 ggcgatcact cccgcgttta tggcggcgct cttcggaacg gccctggctt gtgggacgtt
    13081 gatcgtctcg tcgttcttgg tgtggggcga gcccttcgcc gggtacctgc tcaccggcgg
    13141 cgcgttgtat ttggtgggca cgatcgtgac caccgtcgcc taccacgtgc cgcgcaacga
    13201 ggcgctggcg aggatcgagc cacacggcgc gggcgccgag gaccactggt cgcgctacct
    13261 tcggggttgg accgcatgga accacctgcg tttcgcagcg gccctcgccg cggctgccac
    13321 gctcacgatc gcgctgcgta tcggttgagc gcccgacgtc agatcccagg ccacgacgaa
    13381 tagtggaggc acaccatgtc acagaccagg atcgaaacca cgggccacag ggctccccga
    13441 ccgaggattc ttggttcggc cgtccgcacc ctcgcgctgg gcctggccac cctggccacc
    13501 ggcctcgtgg ccggcgtctt ctgctcgccg tgcacttccc gcagcccagg tcgggccgtt
    13561 tttggctcgt tgcgctggcc tgcgcgctct acatcggggg ctgcttctta ctcacggtct
    13621 tcgtgaacgt acctttgaac gaggaactgg cccgcgtcgc cgagggcgca tcgtccgccg
    13681 aactggccgg cgcgagggcc gcctatgagg gtccttggaa cttctggaac ggtgtgcgga
    13741 cggtgttctc gtcgttggcg ttcctggccc tcatcggcgc ctgcctcttg tgcgaggacc
    13801 gcggcgcatc ctaaccctgc ccgttgctcc tgtcgcccac catacgggag aggagggtgc
    13861 ggaagcgtgg gtcggcgtgc aggggcgcga actccagcgc gctcctgaag tgctcgacgt
    13921 cgcgttcgcc ggcgtcgacg gccttctcga tgtagtcgag cgcgtcgccg tactcgccgc
    13981 ggcgggcctg gatactggcg agcgcggaga gtgcggggga gtatctcgcg tcggcggcta
    14041 tggcccgctc caggatctcc tccgccacct ccaggtcgtc ggccgtgtag tagaggctcg
    14101 cgtacgagta gagcgcacgg gggttcgcgg gatccacctt cagcgactcc acgaagtgct
    14161 cttccgcccg ccccatctcc cccttgcgct cgctcaagat accgagctcg aggtgggcct
    14221 ccgggttctc ggggtcgagg gccaggaccg cgtcgtaggc cgcggccgcc ccgtcttcgt
    14281 cttccagggc gagcctggcc tccccgaggg ttatgaggtg ccggatctca tcccgcccca
    14341 gcgccacggc ctcctccgag agcctcaagg cctctggaag gttcttcctc aaggcgagca
    14401 gatgggccgc cagttcgtcc ctgatctccg gcgagccggg acagtcctcg aggccctgcc
    14461 tgaaggcggc ctccgccttc tccagatcac cgttgcgggc gtggcagatc cccaggttcg
    14521 cgtgggcctc ccaccactcg ggcgccgccg ctatgacctc ctcgtaggcg cctacggctt
    14581 cggcgtagcg gcccagcata tccagggcgt tggccctgaa ggtccgcgcg ccgcacttct
    14641 gcaagtcgtc tgcggcggcc tctatcgcct tctcgaagaa ctcgagggct tcggcgtagt
    14701 cggcggcgtc gaagagggtc aggccggcgt ttatgagcat gtcggcgtct tccggtacgc
    14761 ggttcgtcca gcgctcgaag gtggccttcg ccagcgcggg ctcccc
//
LOCUS       DATMVF010000021        11651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_554072, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000021 DATMVF010000000
VERSION     DATMVF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11651
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554072"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VN178_00725"
     CDS             complement(<1..376)
                     /locus_tag="VN178_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HWS79514.1"
                     /translation="MYEQVYEPIAGNLALSTLVAVIPILVLFVLLAGLRMAAQWASLI
                     TLAVALVIAVVVYGMPIGLALNSTLLGICFGLFPIVWIVINAIFIYNVIVDTGFFDTI
                     RDSLAGVSNDRRIQVVLIAFVFG"
     gene            complement(471..1760)
                     /locus_tag="VN178_00730"
     CDS             complement(471..1760)
                     /locus_tag="VN178_00730"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWS79515.1"
                     /translation="MREDLQEIYAAGLAAADPQRAVRRELELEDGAILAGERRFEPRR
                     VFVLAAGKAAGAMAKAAKELLGEKVSGGLVVTKDGHDPGPEGLETVFASHPEPDERGV
                     EAACRVQELAESLGEDDLLLALISGGASALLADPAAPIELADLKRLTSDLLKSGADIG
                     EINTVRKHVSVLKGGGLVRLAHPAPTIALLLSDVVGDDPSSIASGLTAPDPTTLENTR
                     RVLERYGIEPPDSVVDHLQSAEETPAPDDTIFENVVNRICGGGRHAAEAAAKKAGELG
                     YTPLLLTTTLTGDALGAASMYAAIVREVLASGNPSQPPCAIVSGGEATVTVRGSGTGG
                     PNQEFALALAVELDGVEGWTAFSADTDGNDGSTDATGGIVDGETAQRIADAGMDPEGA
                     LADNDSYAALEAGGALLLTGPTGTNVNDLRVALISRG"
     gene            1830..2645
                     /locus_tag="VN178_00735"
     CDS             1830..2645
                     /locus_tag="VN178_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWS79516.1"
                     /translation="MKFSANVSILFKEVPLLERFGRAAAAGFSAVEFWWPGHEAQLEA
                     VERAVRDAGLDVALFNFDAGDMAAGDRGLVSDPERQHLFTENVPVALDLARSFGCERM
                     NVLVGHEIPGMDREEQLALARENVRFAAQEAEAANVTVMVEAVNTFENGPYLLYTTEQ
                     AVEFVERVGRENVRIQHDFYHMQRMEGNLVANLREHFDHIGHVQIADSPGRGEPGTGE
                     IHYPFVLGELEKLGYDGYVGLEYNPTTGSTEESFGWLPKELRGRDVAVSDLGL"
     gene            2657..3556
                     /locus_tag="VN178_00740"
     CDS             2657..3556
                     /locus_tag="VN178_00740"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HWS79517.1"
                     /translation="MAEAVGFIGLGIMGRPMAENLMEAGYELVVHNRSPEKAEELAKE
                     GNATAAGSPREVAEACDTVITMLPDSPDVEAVVAGEGGVLEGIRDGALLVDMSTISPV
                     VTEELSEKVREKGASMLDAPVSGGDVGAIEGALSIMVGGTEEDFERARPLFDVMGKVA
                     THVGPTGAGQVVKACNQIVVALNIEAVSEALVLGSKAGVAPEKVVEALSGGLAGSAVM
                     EAKKQKFFSHDFEPGFRIELHHKDLGIALVAGREYGVALPVTAIVDQMLEASKAKGRG
                     DRDHSALLTLLEEAAQHEIGEGG"
     gene            3582..5036
                     /locus_tag="VN178_00745"
     CDS             3582..5036
                     /locus_tag="VN178_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWS79518.1"
                     /translation="MERSRLIKEMEGIVGAQGVIHEHEQLRTYECDGLMNYRVIPDLV
                     VLPETGQHVQNIVKLCYDEGIPFVARGSGTGLSGGALPVESGVLIVLSRMRKILEVDI
                     PNQRIVVEPGVVNMWVSQEVADEGYYYAPDPASQIVSSIGGNLAENSGGVHCLKYGFT
                     TNHVMGAEVVLPDGSVTHIGGGRAPDAPGYDLLGAFVGSEGTLGIATRITLRLCRVPE
                     AVHTLLAGFEGTEGAGGAVSGIIGEGIIPAAIEMMDRLTMEAVEEAVHPGLPDAGAVL
                     VIELDGPEAEVEEQFGRVLEICEENEATEIRVAEDDEERALFWKARKAAFGAMGRISN
                     DYYVQDGVIPRTELPAVLGRISELAGEYGLRVANVFHAGDGNLHPLVLYNNDNEGEAE
                     KAEKLAGEIVFACLEHGGSLTGEHGIGEDKKKYMPKMFTDDDMNVMQLLRCAFDPHQL
                     ANPGKIFPTPRLCGERPGPFHMHPLQKAGIVEQF"
     gene            5036..6307
                     /locus_tag="VN178_00750"
     CDS             5036..6307
                     /locus_tag="VN178_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS79519.1"
                     /translation="MQTEKGLAEDLRHIVGQDNLREATEQDAVDGVEPDFVVEPGSIE
                     EISDLMKLAAREGLAVAPRGGGTKMHIGDPPRRLDLIVSTARMNEVLEHTPGDQIIRL
                     EAGVKLEDLQEHISGSDQMLAIDPPESGATIGGIVAANSSGPRRYRYGTIRDLIIGIT
                     VVLHDGTVAKAGGKVVKNVAGYDLSKLFTGSLGTLGVIANANFRLHPRPGSSRTVAVE
                     VTEPQQAQAAAQAIVHSQVEATAVELQYDENEKQLAVLLESIPGGIDAKAETTSFLLK
                     QFGEVRTLSDEEVNHLGPLAPPEVADGVVLKIGAPPAELAAVLVAVLGAAERKGLAHP
                     RITGHAGTGVTLVGFSGENEDGAAGFVEEMREIWVRRGGNVTLQRAPLTLKQRVSTWD
                     NGGDYLGLVRRVKEKFDPRGGMNPGRFLGGN"
     gene            6307..7824
                     /locus_tag="VN178_00755"
     CDS             6307..7824
                     /locus_tag="VN178_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWS79520.1"
                     /translation="MTERNGRGESGDRYDATDATERRSDEGTAREDAMAGAIGAGSPE
                     DVGQSTFAATTDSLRHTMGEDRPESGGYAFPAFDDHHPPDKALIDECVHCGFCLPTCP
                     TYVLFGEEMDSPRGRIYLMNKGLDEEPMNDEMVRHWDLCLGCMACVTACPSGVQYDKL
                     IESTRQQVERRYERRADDKAFREMIFQFFPYPNRLRAVAAPMRLYQRFGIGDKLRRAG
                     VMQRIPARMRAMEALLPDVPKEEKIPELTGPVGEKRRRVGVLTGCVQRVFFSPVNAAT
                     VRVLAAEGCEVVAPKQGCCGALSTHAGREEESLDFARKTIDTFEDLDLDNVVVNAAGC
                     GSTMKEYGYLLRDDPEYAQRAKAFSAKVRDVSEFLQELGPVAERNPLPVTAAYHDACH
                     LAHAQGVRKQPRQTLKQIPGIDVKEIREAEICCGSAGIYNMVEPEPAAELGERKANNI
                     LKTGARIIVTSNPGCMLQIQSSLKKMGHGGLPMAHPVEVLDASLRGEPVETLLDK"
     gene            7826..8722
                     /locus_tag="VN178_00760"
     CDS             7826..8722
                     /locus_tag="VN178_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWS79521.1"
                     /translation="MKAVRSLLAVPATRRKMVEKALASEADAVFLDLEDAVAPDNKAA
                     ARGDVVSALKELGWRGRPGLYRANALDTSYFYRDLIEVVEEAGDSLDAVMIPKVNRPE
                     DLHAVSILLSQLELAMELEKGKIELEAQIESAEGLANVDSIARATDRLTALHFGPGDF
                     AASMSMPQTSIGVMDEWDEAYPGHRFHYAMQRIVVAARAANLRVLDGPVADYRDEEGL
                     RGSCLIARSLGFDGKWCIHPAQIGIVNEVFSPTDREVEWAKKVVAAYEEANAEGSGSV
                     SVDGQMVDAASIRMARTTLDLI"
     gene            8910..9560
                     /locus_tag="VN178_00765"
     CDS             8910..9560
                     /locus_tag="VN178_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HWS79522.1"
                     /translation="MGLDLGLKTSWDIWALRLMTYWVVWAVTYLGLTWFLLVRSSAQQ
                     TRRWALDQRLPPRPHLSILRSVALRILRVLFLVSRTSSLFFIVLVSLVSLVLAISLAP
                     EVRDLQTTRGISLAFMAALGVVLAWGVLHTSYALHYAYLYYRSEESPGGLTFPGEQSP
                     KQRDFAYFAFTIGTSFAVSDVDVTDDAIRQAVLGHQILSFLYNTSILATVVTITTG"
     gene            9715..10467
                     /locus_tag="VN178_00770"
     CDS             9715..10467
                     /locus_tag="VN178_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HWS79523.1"
                     /translation="MNGPEQLPYEARPAGLSARTVRWLRVLLLATAAAVFGLLYLLSA
                     EFRSETNRALGILGRGDIAGLRDYIVSFGLWAPVASCFLMVLQALVAPVPSFLITFAN
                     GLAFGVFWGWMLSLFGHVLAAAVCFGISRALGRVPVEVLVGRTGLQSADRWFARWGVY
                     AVFAGRLLPGVAFDAISFAAGLTRMRFRSFIAATALGIFPQTFLYSYLGRQAPEYVAL
                     FLVTSGLVILGVVVVAVARYRRERRKPKRNLR"
     gene            10514..11146
                     /locus_tag="VN178_00775"
     CDS             10514..11146
                     /locus_tag="VN178_00775"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HWS79524.1"
                     /translation="MAQIFDPPLDYSTRRTGSLIVITGSMFSGKTEEVIRRVRRALYA
                     HRSAQVFKHTIDTRSEDTEIRSHNGVPHRALAVSSSEELLGKVEETTNVVAIEEVQFF
                     DEGIIEVCRRLADAGNEVIAAGLDMDFKGEPFGPMPRLLAEADEVVKLRAICARCGRE
                     ASRSQRLIDGRPAPVSAPTILIGAEESYQARCRHCHEVPEGVFQYGFPGV"
     gene            11167..11598
                     /locus_tag="VN178_00780"
     CDS             11167..11598
                     /locus_tag="VN178_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79525.1"
                     /translation="MPRKYIVVLILFCIALNAAGFLWKLFEPVLFYDEVAHFITPFTL
                     VLILAEIIYRGGGDDEFFDTPMRAIITGSVIGLLGATGWEGIEGMMSLMGISVSNALP
                     DTIFDIFLGVLGGAAGAWAADRYLDRIFGRSRTNANQPRVR"
BASE COUNT         2132 a         3663 c         3833 g         2023 t
ORIGIN      
        1 ccccgaagac gaacgcgata agcacgacct gtatccgccg gtcgttgctg acaccggcga
       61 gcgagtcgcg gatggtgtcg aagaagcccg tgtccacgat gacgttgtag ataaagatcg
      121 cgttgatgac gatccagacg atgggaaaga gcccgaagca gatcccaagg agcgtcgaat
      181 tgagggccag gccgatcggc atcccgtaga ccaccaccgc gatcacgagc gccacggcga
      241 gggtgataag cgatgcccac tgcgccgcca ttcggagccc cgccagcaac acgaacagca
      301 cgagtatcgg gatgaccgcc accagcgtgg agagcgcaag gtttcccgct atgggttcgt
      361 agacttgttc gtacatcagg ctccaatctt cctagaactg tccgatgcgg cggtaagact
      421 tgcggtgcga aagaatggca ccgcaagcgt cacgatgcaa ccctccaact tcaccctcgg
      481 gagatcaggg ccaccctcag atcattgacg ttggtgcctg tcggccctgt gagaagcagg
      541 gctccgcccg cctccagcgc cgcgtacgag tcgttatccg ccagcgcccc ctccgggtcc
      601 atgccggcgt ccgcgatcct ctgcgccgtc tccccgtcca cgatgccacc cgtcgcgtcg
      661 gtagatccgt cgttgccgtc cgtgtcggcc gagaacgcgg tccaaccctc gacgccatcc
      721 agctcgaccg cgagcgccag agcgaactcc tggttcggcc cgccggtgcc gcttccgcgc
      781 acggtaaccg tcgcctcgcc gcccgagacg atggcgcacg gcggctggga cggattcccg
      841 ctcgccagta cctccctgac gatcgccgcg tacatcgagg ccgccccgag ggcgtcgccc
      901 gtgagggtgg tggtgagcag caacggcgtg tatccgagct ctcctgcctt cttcgccgca
      961 gcctcggccg cgtgccggcc gccgccgcag attcgattca cgacgttctc gaagatcgta
     1021 tcgtccggtg ccggagtctc ctcggcgctc tgcagatgat ccacgacgct atccggtggt
     1081 tctatgccgt atcgttccag cacgcgccgg gtgttttcca gggtcgtcgg gtcgggggcg
     1141 gtcaagccgc tggcgatgga agagggatcg tcgccgacca cgtccgagag caggagggct
     1201 atggttgggg caggatgggc cagccgtacc aggccacccc ccttcaacac agagacgtgc
     1261 ttccttacgg tgttgatctc ccctatgtcc gccccgctct tgaggaggtc ggacgtaagc
     1321 cgcttcaggt ccgcgagctc gatcggtgcg gccggatcgg cgaggagcgc cgatgcccca
     1381 cccgagatga gagcgagaag caggtcgtct tctccgagcg actccgcaag ttcctgtact
     1441 ctgcaggccg cctccacacc cctctcgtcg ggctcgggat gggaggcgaa gacggtctcg
     1501 agaccctcgg gacccggatc gtgaccgtct ttggtgacga cgagcccgcc tgagaccttc
     1561 tccccgagaa gctcctttgc ggccttcgcc atcgctcccg ccgccttgcc cgccgcgagt
     1621 acgaacacgc gccgcggctc gaaacgcctc tctccggcca ggatggcgcc gtcttcgagc
     1681 tccagctcgc gccgcacggc ccgctgcggg tcggcggcgg ccaggccggc cgcatatatc
     1741 tcctggaggt cctcgcgcac gagggcattg taaccatccc ggagttcgac gcccggttgg
     1801 gttatgcttg tggcctaagg agggctggta tgaagttcag cgcgaacgtt tccatcttgt
     1861 tcaaagaggt cccattactg gaacggttcg gccgggcggc cgcagcgggt ttttccgcgg
     1921 tcgagttctg gtggcccggt cacgaggcgc agcttgaggc cgtagagcgt gccgtcagag
     1981 acgccggact cgacgtggcg ctcttcaact tcgacgccgg agatatggcg gccggagaca
     2041 gggggctggt gagcgatccc gagcggcaac atctgttcac agagaacgtg cccgtggccc
     2101 tcgatctggc gcgctcgttc ggctgcgaga ggatgaacgt gctggtgggg cacgagatcc
     2161 ccggtatgga ccgcgaagag caactcgcgc tggcacgcga gaacgttcgc ttcgccgcac
     2221 aggaggcaga agcggcgaac gttaccgtta tggtcgaggc cgtcaacacc ttcgagaacg
     2281 ggccctatct cttgtacacc acggagcagg ccgttgagtt cgtggagcgt gtcgggcggg
     2341 agaacgtccg aatccagcac gacttctacc acatgcagcg catggaaggt aacctggtcg
     2401 ccaacctgcg cgagcacttc gaccacatcg ggcacgtcca gatcgcggac tctcccgggc
     2461 gtggggagcc ggggacgggt gagatccact acccgttcgt cctgggcgaa ctcgaaaaac
     2521 tcggctacga tggctacgtc ggcctggagt acaacccgac cacagggtcg accgaggaga
     2581 gcttcggttg gttgccgaaa gaactgcgcg gtagggacgt agccgtgtct gaccttggac
     2641 tttagagagg agcaccatgg ctgaggcggt aggctttata gggctcggga tcatgggccg
     2701 gccgatggcg gagaacctga tggaggcggg ctacgaactg gtcgtccaca accgcagtcc
     2761 tgagaaggcg gaagaactgg cgaaggaagg caacgccacc gctgccggca gcccgaggga
     2821 ggtggcggag gcgtgcgaca ctgtcatcac catgctcccc gactcgcctg acgtcgaggc
     2881 cgtggtcgcc ggggagggcg gcgtcctcga aggtatccgc gacggcgctc tgctcgtgga
     2941 tatgagcacg atctcgcccg tggtcaccga ggagctttcg gagaaggtcc gagagaaggg
     3001 cgcctcgatg ttggacgcgc cggtctccgg gggcgacgtg ggggccatag agggcgcgct
     3061 ctccatcatg gtgggcggta cggaggagga cttcgagcgc gcccgtcccc tgttcgacgt
     3121 gatggggaag gtggcgacgc atgtgggacc caccggagcc ggtcaggttg tcaaggcttg
     3181 caaccagatc gtggtggccc tcaacatcga ggctgtctcg gaggctctgg tgctcgggtc
     3241 gaaggccggc gtcgcgccgg agaaggtggt ggaggcgctc tccggggggc tggcgggcag
     3301 cgcggtgatg gaggccaaga agcagaagtt cttctcccac gacttcgagc cgggtttcag
     3361 gatagaattg caccacaagg atctgggtat agcccttgtg gcgggcaggg aatatggtgt
     3421 ggccctgccg gtcacggcca tcgtggacca gatgctcgaa gcgtcgaagg ccaagggccg
     3481 gggcgatagg gaccactcgg cgctactcac cttgctggag gaagccgcac aacacgagat
     3541 aggggaggga ggctgaagct tcccgccgaa ggagggcaaa catggagcgt agccggctca
     3601 taaaggagat ggaaggcatc gtgggcgccc agggtgtgat ccacgagcac gagcagctac
     3661 gcacctacga gtgcgacggc ctgatgaact accgcgtcat ccccgacctc gtcgtcctcc
     3721 ccgagaccgg ccaacacgtc cagaacatag tcaagctctg ctacgacgag gggattccct
     3781 tcgtcgcccg cggctcgggg acaggcctct cgggcggggc actgccggtc gagtcgggcg
     3841 tcctgatcgt gctctccagg atgcgcaaga ttctagaagt ggacatcccg aaccagcgta
     3901 tcgtggtcga gcccggcgtc gtcaacatgt gggtgagcca ggaggtcgcc gacgaaggct
     3961 actactacgc gccggacccg gccagccaga tcgtctcctc catcggcggc aacctggcgg
     4021 agaactccgg cggcgtccac tgcctgaagt acggctttac gacgaaccac gtgatgggcg
     4081 cggaggtcgt gctgcccgac ggctcggtga cgcacatcgg gggcggccgg gcgccggatg
     4141 cgcccggtta cgacctgctc ggcgctttcg tagggtcgga ggggacgctt ggtatcgcca
     4201 ccaggatcac actccgtctg tgccgcgtcc ccgaggcggt gcacaccttg cttgccggct
     4261 tcgaaggtac cgagggggcc ggcggcgccg tctcggggat catcggcgaa gggataatcc
     4321 ccgccgccat cgagatgatg gaccgcctga ccatggaggc cgtcgaggag gccgttcacc
     4381 ctggcctgcc cgacgctggc gcggtgctcg ttatcgagct ggacgggcct gaagctgagg
     4441 tggaggaaca gttcggacgg gtgctcgaga tctgcgagga gaacgaggcc acggagatcc
     4501 gggtggccga ggacgacgag gaacgcgccc tcttctggaa ggcccgtaag gccgctttcg
     4561 gcgccatggg ccgcatctcc aacgactact acgtccagga cggcgtcatc ccccgcacgg
     4621 agctgcccgc ggtcctcggc aggatctccg agctcgccgg agagtacggc ctgcgggtcg
     4681 ccaacgtgtt ccacgccggg gacggtaacc tgcatcccct ggtcctctac aacaacgata
     4741 acgagggcga ggccgagaag gccgagaagc tggccggcga gatcgtcttc gcctgcctgg
     4801 agcacggcgg gtcgctcacc ggggagcacg gcatagggga ggacaagaag aagtacatgc
     4861 ccaagatgtt caccgacgac gacatgaacg tcatgcagct cctgcggtgc gcgttcgatc
     4921 cgcaccagtt ggccaacccg gggaagatct tccccacccc ccgcctctgc ggcgaacgtc
     4981 ctggcccctt ccacatgcac ccactgcaga aggccggcat cgtggagcag ttctgatgca
     5041 gacggagaaa ggactcgcag aggacctcag acacatagtc ggccaggaca acctccgcga
     5101 agcgacggaa caggacgccg tagatggcgt cgaacccgac ttcgtggtcg agcccggctc
     5161 catcgaggag atcagcgacc tgatgaagct cgccgcccgc gagggcctgg ccgtcgcgcc
     5221 ccgcggcggc ggcacgaaga tgcacatcgg cgacccgccg cgccggctcg acctcatcgt
     5281 gagcaccgcg cgcatgaacg aggtgctgga acacacgccg ggagaccaga tcatccgcct
     5341 cgaggccggg gtaaagctgg aagacctgca agaacacatc tccggctcgg accagatgct
     5401 cgccatagac ccgccggaga gcggggccac catcggcgga atagtcgccg ccaactcttc
     5461 cgggccgagg cggtacaggt acggcaccat acgggacctg atcatcggca taaccgtcgt
     5521 cctgcacgac ggtaccgtgg ccaaggcagg cggcaaggtc gtcaagaacg tggccggcta
     5581 tgacctgtcg aagctcttta ccggctccct gggcaccctc ggcgtcatcg ccaacgccaa
     5641 cttccgcctc catccacgtc ccggctcctc ccgcaccgtg gccgtcgaag tcacagaacc
     5701 ccagcaggcg caggctgcgg cgcaggctat agttcattct caggttgagg ctacggcggt
     5761 cgagttgcag tatgacgaga acgagaaaca gctggcggtg ctgttggaga gcatccccgg
     5821 aggcatagac gccaaggcgg agaccacctc cttcctcctc aagcaattcg gggaggtgcg
     5881 gacgctctcg gacgaggagg tgaatcatct cggccccctc gcgccgcccg aggtcgccga
     5941 cggggtcgtc ctgaagatcg gtgctccgcc ggcggagctg gccgccgtgc tggtagcggt
     6001 ccttggcgcg gcggagcgca agggactcgc gcatccgcgc atcacgggtc acgccgggac
     6061 cggtgtcacc ctcgtcggct tctccgggga gaacgaagac ggcgcggccg ggttcgtgga
     6121 ggagatgcga gagatctggg ttcgccgcgg cgggaacgtg accctgcaac gcgccccatt
     6181 aaccctgaag caaagagtta gtacgtggga caacggggga gattatctcg gtctcgtgcg
     6241 gagggtgaag gagaagttcg atccccgggg cggcatgaac cccggcaggt tcctgggagg
     6301 taactagtga cagagcgaaa cggtcggggc gaatccggcg accgctacga cgcgacggac
     6361 gctacggagc gccggagcga cgaggggacg gcgcgtgagg acgctatggc tggggccatc
     6421 ggcgccggga gcccggagga cgtggggcag agcaccttcg cggcgaccac ggactctctg
     6481 cggcacacga tgggcgagga ccggcccgag agcggcggct atgcgttccc ggccttcgac
     6541 gaccaccatc cgccggacaa ggccctcata gacgagtgcg tgcattgtgg cttctgcctg
     6601 ccgacgtgcc cgacgtacgt gttgttcggg gaagagatgg actccccgcg cgggcgcatc
     6661 tacctgatga acaagggcct cgacgaggag ccgatgaacg acgagatggt ccgccactgg
     6721 gatctgtgcc tggggtgcat ggcctgcgtt acggcctgcc cgtcaggcgt gcagtacgac
     6781 aagctcatcg agtccacgcg ccagcaggtc gagcgccgct acgagcggcg ggccgacgac
     6841 aaggcgttca gggagatgat cttccagttc ttcccctacc ccaaccgcct tcgggccgtc
     6901 gcggcaccga tgcgcctcta ccagcgcttc gggatcgggg acaagctgcg cagggccggc
     6961 gtcatgcaac gcatcccggc caggatgcgg gctatggagg ctctactgcc cgatgtgccg
     7021 aaggaagaga agatccccga gttaacaggc cctgtgggcg agaaacgccg cagggtcggc
     7081 gtattaacgg gctgcgtgca gcgggtcttc ttctccccgg tcaacgccgc cacggtgcgc
     7141 gtcctggccg ccgagggttg tgaggtggtg gctcccaagc agggttgttg cggcgccctt
     7201 tccacccacg cgggccgcga ggaggagtcc ctggacttcg cccgcaagac catagacacc
     7261 ttcgaggacc tggacctgga caacgtcgtc gtcaacgccg cgggctgtgg ctcgacgatg
     7321 aaggagtacg gttacctgct gcgcgacgac cccgagtacg ctcagagggc gaaggccttc
     7381 agtgcgaagg tccgcgatgt ctcggagttt ttgcaggagc tcggtcctgt ggccgagcga
     7441 aatccgttgc ctgttacggc ggcctaccac gatgcctgcc acctggcgca cgcgcaggga
     7501 gtacgcaagc aaccgcgcca gaccctcaag cagattccag ggatagacgt aaaggagatc
     7561 agggaggccg agatctgctg cggatcagcg ggcatctaca acatggtcga gcccgagccg
     7621 gccgccgaac tcggcgagcg caaggcgaac aacatcctga agaccggggc gcggattatc
     7681 gttacctcca accccggctg catgctccag atccagtcga gcctgaagaa gatgggccac
     7741 gggggtcttc cgatggccca ccccgtggag gttctggacg cgtccctgcg cggagagccc
     7801 gtagagacgc tcctcgacaa gtagtatgaa agccgtccgt tccctcctcg ccgtccccgc
     7861 aacgcggcgg aagatggtcg agaaggcgct cgcctcggag gccgacgccg tgttcctcga
     7921 tctcgaagat gccgtagccc ccgacaacaa ggccgcggcc cgcggggacg ttgtctccgc
     7981 tctaaaagag ctcggctggc gtggtcgtcc gggactctac agggccaacg cactggacac
     8041 gtcttacttc tacagggatc tgatcgaggt ggtcgaagag gccggcgact ctttagacgc
     8101 cgtcatgatc cccaaagtga accgccccga agatctccac gctgtctcta tcctcctctc
     8161 ccagctggag ctggctatgg agctggagaa gggcaagata gagctcgaag cccagataga
     8221 gagcgccgaa ggcctggcca acgtggactc tatagcccgc gccacggacc gcctcacggc
     8281 cctccacttc ggtcctggag atttcgctgc cagcatgagc atgccccaga cgagtatcgg
     8341 cgtgatggac gagtgggacg aagcgtatcc cggccaccgc ttccactacg ccatgcagcg
     8401 catcgtggtg gcggcccgcg ccgcgaacct gcgcgtcctc gacggtcccg tcgccgacta
     8461 ccgcgacgag gaggggttgc gtgggagctg cctgatcgcc cgctctctgg gcttcgacgg
     8521 taagtggtgc atccaccccg cgcagatagg gatcgtcaac gaggtcttct cgcccaccga
     8581 tcgggaagta gagtgggcga agaaggtcgt cgcagcctac gaggaggcga acgccgaagg
     8641 ctcgggctcg gtaagcgtag acgggcagat ggtagacgcc gcctccataa ggatggcgcg
     8701 aaccacgctc gacctgatct aggacctgtt atcggcattc agcgaccagc aaagggcaag
     8761 gccgaccgct acgaaggatt atgtatgcta cgccttgtgc actgcctatg tctgctacgg
     8821 ggcggggaaa cagtttgacg gatattgggc ggatactgat agccgcgatc accgcccttg
     8881 tcgtcgtcta cgcgctgcgg ccttgggcaa tgggtttaga ccttgggttg aagacctcct
     8941 gggacatatg ggccctcagg ttgatgacct actgggtcgt ctgggcggtt acttacttgg
     9001 ggcttacctg gtttctgcta gtccgctctt ccgcccagca gacccgtcga tgggctttag
     9061 accagcgatt accacctcgc ccgcaccttt ccatactgcg ctcggtggca ctgcgaatac
     9121 tgcgggtact gttcttggtt agtcggacga gctctttgtt ctttatcgtc ctcgtaagtc
     9181 tggttagtct ggtgctggcg atcagccttg cgcccgaggt acgcgacctg caaaccacac
     9241 gaggcatctc tctggccttc atggccgcgt taggagtggt cttggcgtgg ggagtgcttc
     9301 atacctccta cgcgctccac tacgcttacc tttactatcg atccgaagaa tcgcccggag
     9361 ggcttacctt tcctggcgag cagagtccga agcaacgcga ctttgcctat ttcgccttta
     9421 ccatcggcac gagttttgcc gtctccgacg tcgatgtaac cgacgatgcg atacgccagg
     9481 cagtcctagg acatcaaatt ctctcgtttc tctacaacac ctcgatcctg gccacagtgg
     9541 taacgataac taccggctaa tgagcttcgg ttaacgagtc cgatgacgcc acctcaccgg
     9601 ccaccaatga atagagagtc gcagagcgct tctccgacct ctcgactcgc gaacgagcga
     9661 ggagccgcca tcttccaaag ttcttcaggt ccggcggcgg tataacatgg accgatgaac
     9721 gggcccgagc aactacctta cgaggcgcga ccggcaggac ttagcgcccg tacggtgcgc
     9781 tggttgcggg tcttgttgct cgctacggca gcggcggtct tcgggctgct gtatctcctg
     9841 tcggcggagt tccggtccga gacgaatcgg gcgttgggga tcctgggtcg tggagacatc
     9901 gccgggctca gagactacat cgtctcgttc ggtctgtggg cgcctgtggc ctcgtgtttt
     9961 cttatggtat tgcaggcttt ggttgcgcct gtaccatcct tcctcatcac gttcgccaac
    10021 gggctcgctt tcggggtgtt ctggggctgg atgctctccc tgttcgggca cgtgctcgcg
    10081 gcggccgtgt gcttcgggat ctcgcgcgcc ctcggcaggg tccccgtaga agtgctcgtc
    10141 gggaggacgg ggctccaatc ggccgaccgc tggttcgcgc ggtggggagt ctacgccgta
    10201 ttcgccggac ggcttctacc cggtgtcgct ttcgacgcca tctcctttgc agcgggactc
    10261 acccggatgc gcttcagaag cttcatagcg gccacggccc tcggcatctt cccccagaca
    10321 ttcctctact cgtacctggg gcgccaggcg cccgagtacg tcgcactgtt tttggtgacc
    10381 tcgggtctcg tgatcctcgg agtagtagtg gtcgccgttg ccaggtacag gagggaacgc
    10441 cgcaagccca aacggaacct gcgctagcga agcagatcct gtggacggct gggcggaggg
    10501 tagaataagc accatggcgc agatcttcga tccaccgcta gattattcga cccggagaac
    10561 cgggtccctc atcgtcatta cgggctcgat gttctcgggc aagactgagg aggtgatccg
    10621 acgcgtacgc agggccctct acgcccaccg cagcgcacag gtcttcaagc acaccatcga
    10681 cacccgctcg gaggacaccg agatacgctc ccacaacggg gtcccgcaca gagcgctcgc
    10741 cgtctcgagt agcgaagagc tactagggaa ggtagaggag actaccaacg ttgtggccat
    10801 cgaagaagtg cagttcttcg acgaggggat catagaggtc tgcaggcgac tggccgatgc
    10861 gggaaacgag gtaatcgcgg cgggcctcga catggatttc aagggcgagc cattcgggcc
    10921 gatgccgagg ctgctggccg aggccgacga ggtggtaaag ctgagggcca tctgcgcccg
    10981 ctgcggcagg gaagcctcgc gctcacagcg tctcatcgac ggaagaccgg cccccgtctc
    11041 cgcgccgacc atcctgatag gggccgagga gagctaccag gcacgctgcc gccactgcca
    11101 cgaggtgccc gagggcgtgt tccagtacgg gttccccggt gtttagcgaa ccgtggtgac
    11161 aggataatgc cgcgaaaata catcgtggtc ctgatcctgt tctgcatcgc tttgaacgcg
    11221 gccggctttc tgtggaagct cttcgaaccc gttctcttct acgacgaggt cgcccacttc
    11281 atcacgcctt ttacgctcgt gctgatactg gccgagatca tctaccgtgg tgggggagac
    11341 gacgagttct tcgacacacc gatgcgggcc atcatcacag gctctgtcat cggtctcctc
    11401 ggcgccacgg gttgggaggg catcgagggc atgatgtcct tgatgggcat tagcgtctcc
    11461 aacgccctgc cagatacgat attcgacatc ttcctgggcg tcctcggcgg cgccgctggc
    11521 gcgtgggccg cggaccgcta cctggaccgt atcttcggac gctcgcgcac gaacgccaac
    11581 cagccccgcg tccgctgaag agcacgtcag cacgctccag cctgcggctg tcgcttgtca
    11641 gcatgtcagc a
//
LOCUS       DATMVF010000022        14226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_598805, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000022 DATMVF010000000
VERSION     DATMVF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598805"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VN178_00785"
     CDS             <1..250
                     /locus_tag="VN178_00785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS79526.1"
                     /translation="MQGRGIGSRLLRAGIGRFPLSTSLVLRVERDNTRAQRFYEAHGF
                     RHAADHAEEICGHVVDVAEMILHLPGSTHRTVPSNGRT"
     gene            395..1549
                     /locus_tag="VN178_00790"
     CDS             395..1549
                     /locus_tag="VN178_00790"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWS79527.1"
                     /translation="MSLSVLTRVLPDSRIWGVRGRFEEVFGRLGAGVWPILQTAVAAS
                     LAWFLASAALGHDRPFFAAIAAVVSTGVVVGQEGRRAIELVFGVACGLAVADLLVLAI
                     GTGTVQIGIVVALAMVAAMMLGGGTLLITEAGVSALLAITLEPTTTGLSPDRFIDALV
                     GSGVALAVRAVFPSDPRHIVERAAHPIFDDLVDALEETAAALRECDLGRAERALQKAR
                     EMDGRMGAFREALDAGYDTVKLSPPRRRALGQLGLYATAADQLDLAVRNVRVLARAVV
                     AAVREGESAPEALSETIRDLARAVETLSAYIEQPEHPLETRRFALEAAIGATTVLSER
                     NDLSTSMLVGQIRSTALDLLRASGMDSTESLEALRDAANRTTREPTNQIV"
     gene            1667..2503
                     /locus_tag="VN178_00795"
     CDS             1667..2503
                     /locus_tag="VN178_00795"
                     /inference="COORDINATES: protein motif:HMM:NF009499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS79528.1"
                     /translation="MSDASSAVSTNPARSSSVSGPGDLRGRVALVTGVGRKKGIGSAV
                     CLALASRGADVVLSYWKAYDREMPWASDEDEPGALLGQLRAAGVGAEGIEMDLSRADS
                     ARLLLDATEERLGRPSILVNTAAYSTRDGFETLDAEALDAHYAVNVRAMALLSVGFAR
                     RYTGGPGGRIVNFSSGQSLGPMPGELAYVATKGAIEAFTRTLAAEVGHKQITVNAIDP
                     GPTDTGWMTEELKREIAPKFPSGRIGQPEDAARLVAFLAGDEAAWITGQVIHSEGGFL
                     RT"
     gene            2544..2951
                     /locus_tag="VN178_00800"
     CDS             2544..2951
                     /locus_tag="VN178_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79529.1"
                     /translation="MKVPRKLPRLFREPPRTLFDDPRHETFEAPVKGPLFTNSVFYAL
                     LAVFGIFLMLTADWLGLLLLNSDGVFRRIGPLNTVRVTAVLMTGIAIPSALLMFHYKR
                     LYSYTSDENVFFAVTFSLSFVLGFPCVLTGIFG"
     gene            3168..4472
                     /locus_tag="VN178_00805"
     CDS             3168..4472
                     /locus_tag="VN178_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWS79530.1"
                     /translation="MGPEEWRRRVHRAQRGRREQQRELGVVPDDEIHPTDYVPRILPA
                     GDWAVLERGLAQRMLAINEWLRRLEAGKDEVVPQEVIESSTLFDASIPTRFGDVPNRQ
                     MGFDIVTVERSGRPGEWEYLVIEDNAKMPVGIEPMNILRAGTPDVLSESYEALRVRPL
                     DGLMERLGEVVRSASPKPDPTLAILSTGPHDQYYLDHKIFASELGAILAERHEVELDR
                     DGRLVHRKSGQRIDVIYERIEDGRIYDDIKGLIESQASGLIDAVFAPNLGVADDKGVY
                     PFIPEMIRTYLGEEPVLDNVETYSLAVQDDRRYVMDHFDELVVKSRSGWGGKGVLIAP
                     EETREDVEEFRKQVEDNPVEYVAQETIDFSTHVLCETDEANDSFVLRDAHADYRIHAL
                     APDAQTIEVIPGAMTRVAAPGSKLVNISSGGKMKDTWVLRGT"
     gene            complement(4605..5012)
                     /locus_tag="VN178_00810"
     CDS             complement(4605..5012)
                     /locus_tag="VN178_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWS79531.1"
                     /translation="MQSFYREILLDHFEHPRNRRELEDADIHEHIDNPLCGDVVTVYA
                     RAHDGVFEEVSFTGRGCSISQASASMLTEQLTGKSREEVEETIEAFLEMMRTEEREEL
                     GELAALKGIVQTPNRIRCATLAWDAMRRALTTL"
     gene            complement(5102..5449)
                     /locus_tag="VN178_00815"
     CDS             complement(5102..5449)
                     /locus_tag="VN178_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HWS79532.1"
                     /translation="MAEFHKIARIADVAPGEVGVYELEGHRVALCNVDGEFYAIEDVC
                     THDAGPLDQGELIGNQIKCPRHGAKFDVTTGKALTLPAIRPVPKHEVKVEGDDIYVAL
                     AEAKVTGTRRRRR"
     gene            complement(5454..6818)
                     /gene="sufD"
                     /locus_tag="VN178_00820"
     CDS             complement(5454..6818)
                     /gene="sufD"
                     /locus_tag="VN178_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HWS79533.1"
                     /translation="MQKEAPEVLKGSRMSEETVKALSSFKDEPGWLTEKRLGAWRAFE
                     ALPMPTLRDEAWRYTDISDVNPEDFSPYVPSPDVSSEGDLPDAVQKLIREGEENSALL
                     VQHNSETTYQRMDDELQNKGVVFTDLHTALREHEDLIQEKLFRLVPEDYDKFSALSAA
                     LFAGGSFLYVPRGVDVEVPIQSYRWLDAIGATMPRTLVIVEENASVTYIDEYASDGTE
                     EDEPALSNGAVELYVGQGANLRYVSLQNWGRNVLHFNTIRSEACRDATINSLVVALGS
                     ELSRTNVDAGLAEAGSDSEMLGLYFADSNQVLDHHTLQDHLAPNAHSDLLYKGALRDE
                     SLAVFSGLIRVEPGAQKTDAYQTNRNLILGTDDAMAVSLPRLEIQADDVKCSHGSTTG
                     QVDDVELFYLMSRGIPRREAEKLVVFGFFGEVTSRIPLKGLKDKLDRAIEGKIGLGFE
                     ERVA"
     gene            complement(6919..8331)
                     /gene="sufB"
                     /locus_tag="VN178_00825"
     CDS             complement(6919..8331)
                     /gene="sufB"
                     /locus_tag="VN178_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWS79534.1"
                     /translation="MPEDKTIQELGLDEYKYGFRDPEEYFFRTPRKGIDPEIVAMISK
                     HKNEPEWMLEFRLNALESFLEKPTPTWGGDLSGMDYDDIYYYIRPMENQGRSWDDVPD
                     DIKNTFEKLGIPQAERESLAGVGAQYESEVVYHSLKKELEEQGVIFLDMDGGLREHED
                     IVKEYFGTVIPPDDNKFAALNSAVWSGGSFVYVPPGVSIDIPLQAYFRINAENMGQFE
                     RTLIIADEDSYVHYIEGCTAPTYTTNSLHSAVVELIAKPGARIRYSTIQNWSHNTYNL
                     VTKRGVAHENATIEWVDGNLGSKLTMKYPAVYLVGEGARGEILSVAYAGDGQHQDAGG
                     KVVHAAPNTSSLITSKSISKGTGRSTYRGLLKVHEGCHDSKSRVECDALLLDENARTD
                     TYPYIEIEEKKVNTEHEATVSKVGEDQLFYLMSRGLSEEEAIAMVVNGFIEPIAKELP
                     LEYAIELNRLIQLEMEGSVG"
     gene            complement(8347..9111)
                     /gene="sufC"
                     /locus_tag="VN178_00830"
     CDS             complement(8347..9111)
                     /gene="sufC"
                     /locus_tag="VN178_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWS79535.1"
                     /translation="MLKIEDLHVEIDGQEIVKGLDLEVGKGEIHAIMGPNGSGKSTLA
                     NVLMGHPRYEVTGGSITFQDEDVFELEPDERAKLGMFLAFQYPSEVPGVSVANFLRTA
                     VNSVREEELTPMEMYRLLQEKMGIMQMDPKFAERYLNEGFSGGEKKRNEILQMLMLDP
                     KLAIMDETDSGLDIDALQVVAKGVNELRGPEFSAVIITHYQRILRYIEPDHVHVMLDG
                     RLVTSGGKKLAEELEEKGYDWVRQEFGAGASEASRS"
     gene            complement(9445..10443)
                     /locus_tag="VN178_00835"
     CDS             complement(9445..10443)
                     /locus_tag="VN178_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79536.1"
                     /translation="MEYSKDPQNNADPNDLEGYAALRDGAAIVDRSDRRLLRLTGKDP
                     IGMLNAVLTSDVPTQEDRGAYAMLLDPKGRIQTDLRVVKAGDEILIDTEPEGAKAAKE
                     ILTRYAPFSRVRLEELSWSVLGLYGPRAREYFGDLEEHQSREIEIEGRILLSVGVAFP
                     VPGYDLIGPPEDLAAVREYLNETGATTVSADAYETARIEAGVPRFGADITPENFPGES
                     KNSLKRAISFEKGCYPGQETVARMHYRGSPNKRLYRFELEQGSTEPPEAGDEILQGER
                     KLVGRVSSADVVGRLSSVAPLPVEGKIYTLGYLARKADLEAPMNAQDARVLGVEPA"
     gene            10475..11116
                     /locus_tag="VN178_00840"
     CDS             10475..11116
                     /locus_tag="VN178_00840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWS79537.1"
                     /translation="MVKVVVLDVDGTLMDTNYLHTEAWARAFEGAGHRVPRVRLHKQV
                     GKGAGLLIREFVDDEEKVEKIQNLHSEVYAELQEYGHPLPGAKELISSLKERGYEVWL
                     VTSAKDEELEHHMQELEAGESVDGVVNSSAVENPKPAPDIFEETLRRAGSTADETVAV
                     GDAVWDVEAAREAGIRTVAVLSGGAYHRDDLEEAGAVAVYEDCAALLDSDFPE"
     gene            11259..11462
                     /locus_tag="VN178_00845"
     CDS             11259..11462
                     /locus_tag="VN178_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWS79538.1"
                     /translation="MAQGTVKWFSDEKGYGFISPSDGGEDLFVHYTGIEGSGFKTLEE
                     GALVTFETSQGKKGLQAVNVSPA"
     gene            complement(11594..12796)
                     /gene="larC"
                     /locus_tag="VN178_00850"
     CDS             complement(11594..12796)
                     /gene="larC"
                     /locus_tag="VN178_00850"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HWS79539.1"
                     /translation="MKHVHFQPIGGAAGDMTLAALVAAGAPLPEITALLRGLGVSFDL
                     AVQRVEVNGIGTLRADVGYPGEHPHRTFGDIRACIEGAALPERASSRALEAFRLLAVA
                     EGAVHDEDPEEVTFHEVGAVDSIVDIVGSCVALELLGAASVSCGPLPMGTGVVDAAHG
                     PLPVPGPATLEILEGSPVRWTDEPVETTTPTGAALMRSFTGGEFTDAAPPMTLHATGY
                     GTGRARLRSAPNLLRVVVGELEGPVGAIEVLEANVDDAPGEILGAAVERLLAAGALDA
                     WLEPIVMKRGRGAYKVCALALTGDRERLAWLLMRETGTLGVRHHTVGRTVAGRRRVVV
                     DLPYGRCGVKVGSIEGEDFVAAPEYADAARLAKETGLPLPRVYEDARTAFEIRLQASG
                     FRFQEEPD"
     gene            complement(12869..13216)
                     /locus_tag="VN178_00855"
     CDS             complement(12869..13216)
                     /locus_tag="VN178_00855"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HWS79540.1"
                     /translation="MNEIPERIEREMFEIRSRMAPDVRDLKKHAEPQVIVKRTSTKVK
                     ERVRDAVIRFGKNLKESASRQAKMIGEAGRKRNPAPLTDAVKRDPRPLIVLAISLAVT
                     LMAVRKISNGREE"
     gene            complement(13216..13653)
                     /locus_tag="VN178_00860"
     CDS             complement(13216..13653)
                     /locus_tag="VN178_00860"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWS79541.1"
                     /translation="MDGRERVAEFGRELREGPEPSDRSIKEIVDALRPQLQELVDKQL
                     ELAKTEMTPIAKRGGLASGLLAAAGVFMLVFLIIFSLAGVYLLDVFLSLWVSALIVSG
                     VLLLVGGILGAAGAGILRRLDPTPHRTIRTLQQNINWIKGQLR"
     gene            13882..>14226
                     /locus_tag="VN178_00865"
     CDS             13882..>14226
                     /locus_tag="VN178_00865"
                     /inference="COORDINATES: protein motif:HMM:NF040059.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79542.1"
                     /translation="MKLHVALGISSGDVAAFVGAGGKSGAILAVSGGLTEEGMKVLVA
                     PTTKMLTSEADSIGPLVTSEDAGDLRARTERALSSAPAVVVGSGMLSKNRVGGVDPEW
                     VGDLAGLADVVLV"
BASE COUNT         2498 a         4430 c         4509 g         2789 t
ORIGIN      
        1 aatgcaggga cggggaatag gatcccgcct cctccgagcc ggcattggca ggttcccact
       61 ctctacgagc ctcgtgctac gggtggagcg ggataacacg cgggcccaac gcttctacga
      121 ggcccacggt ttcagacacg ccgcagatca cgctgaggag atctgcgggc acgtggttga
      181 cgtggcggag atgatcctgc acctaccagg ttcaacccac cggaccgtcc ccagcaacgg
      241 tcgaacttag agaaagtaac aatcaagtaa cagcacggta acaaatcgtt agaacacctc
      301 ccgaacccct ttgaccgcca gggtcctcgt gatagtgtcg ctagtgtgcg gagcgcatat
      361 aggtactggt tgaagctgaa ggaggtgttt ggttgtgagc ctgagcgtgc ttacgcgggt
      421 tctgcctgat agtaggatct ggggggtacg aggtcggttc gaggaggtgt tcggccggtt
      481 gggggccggg gtatggccca tcttgcagac ggcggttgcg gcgagtctgg cgtggtttct
      541 ggcttctgct gcgctcgggc acgaccggcc gtttttcgcc gcgatagccg ctgtagtctc
      601 taccggcgtc gtcgtcggtc aggaggggcg gcgggcgatc gagctcgtgt tcggggtcgc
      661 gtgcggcctt gcggtcgccg acctcctggt gctcgccatc gggacgggga cggtccagat
      721 cgggatcgtc gtggccctgg ctatggttgc agccatgatg ctcggtggtg ggacgttgct
      781 aatcactgag gccggggttt ctgcgctgtt ggcgatcacg ctcgagccta ccacaacggg
      841 tctttcgcct gacaggttta tcgacgcgtt ggtagggagt ggggtggccc tcgcggtcag
      901 ggctgtgttc ccgagcgacc cgaggcacat cgtagagcgg gccgcgcatc cgatcttcga
      961 tgacctcgtc gacgccctcg aagagaccgc ggcggccctg cgagagtgcg acctcggccg
     1021 ggccgagcgc gccttgcaga aggcgcgtga gatggacgga cgcatgggcg ctttcaggga
     1081 ggcgctcgac gccggctacg ataccgtcaa gctctctccg ccgcgcaggc gggcgctcgg
     1141 acagctcggg ctctacgcta cggcggccga ccagttggac ctcgcggtcc gcaacgtcag
     1201 ggtactcgcg cgggccgtcg tggcggcggt acgcgaggga gagtctgccc cggaggcgct
     1261 ctccgagacc atccgcgacc tcgcccgggc cgtcgaaaca ctctcggcct atatagaaca
     1321 accggagcat cccttggaga cccgcaggtt cgccctcgag gccgcgatag gggcgaccac
     1381 cgtactctcg gagcgtaacg atctcagtac cagcatgctg gtgggccaga tcaggtcgac
     1441 cgctctggac ctgctgcgag cctctggcat ggactcgacc gagtccctcg aagcgctccg
     1501 cgatgccgcc aaccgtacga cccgggagcc cacgaaccag atcgtctgag ccggggagat
     1561 gtatgggtcc ggcgtatgat cccggcttga ggtgcgcttg ggggcatgct aggtttacct
     1621 ctatccgcca ctcccgtccg gcgccatcta tgtcgaggag aagcccatgt ccgatgccag
     1681 ctccgcggtc tccaccaacc cggcccgctc ttcctcagtt tccggaccag gagatttgcg
     1741 aggtcgtgtg gcgctcgtga ccggtgtcgg aaggaagaag gggataggct ccgcggtctg
     1801 cctggcgctc gcctcccgag gcgccgacgt cgttctctcc tactggaaag cttacgaccg
     1861 cgagatgccc tgggcgtcgg acgaggacga gcctggggca ttgctcgggc agttgcgtgc
     1921 cgccggagta ggggcagagg ggatagagat ggacctctcc agggccgatt cggcgcggct
     1981 ccttctcgac gcgaccgagg agaggctcgg gaggccctcg atcctcgtaa acaccgccgc
     2041 ttactccacc cgcgacggct tcgagacgct cgacgcagag gcgctggacg cccactacgc
     2101 cgtcaacgtg cgggccatgg ccctccttag cgtaggcttc gcaaggaggt atactggcgg
     2161 gcctgggggc aggatcgtca actttagttc gggacagtcg ctagggccga tgcccggaga
     2221 gctcgcctac gtcgcaacca agggcgccat cgaggcgttc accaggacac tcgccgccga
     2281 ggtcgggcac aagcagatca cggtgaacgc catcgaccct ggtcctacgg acacgggctg
     2341 gatgaccgag gagttgaagc gcgagatcgc accgaagttc ccctccggga ggatcggaca
     2401 gcctgaggac gcggcgaggc tcgtggcgtt tttggccgga gatgaggcgg cctggatcac
     2461 gggccaggtc atccattccg agggcggctt cttacgaact tgagcggcgc gttcgcttcc
     2521 cttgcgctac cattacgtct cgaatgaagg ttcccagaaa gttacccagg ctcttccgcg
     2581 aaccgccgag gacgctgttc gacgacccgc gccacgaaac cttcgaggcg cccgtcaagg
     2641 gaccgttgtt cacgaactcc gtgttctacg cgctcctggc cgtcttcggc atcttcctca
     2701 tgcttaccgc cgactggctc ggtctgctgc tcttgaattc ggacggcgtc ttccgtagga
     2761 tcggtccgct caacaccgtg cgcgtgacgg ccgtcctgat gaccgggata gccatcccct
     2821 cggctctcct gatgttccac tacaaacgcc tctactccta tacctctgac gagaatgtct
     2881 tcttcgccgt caccttctcg ctctcattcg tgctgggctt cccctgcgtc ctcacgggga
     2941 tattcggatg aagaaggcat caggtatcag gcatcaggct tcaggagggt ttgatcgcct
     3001 ggccggcgtc cgtcctcgaa ccgtactgct gaagcctgaa gcccaaactc tagctgatgc
     3061 ctgaccgctt gagattcgaa cgcggcctct cctcaccgaa cgaagtcttc agtgctgacg
     3121 gcgagacccg cgctcattac cagcctgtgc tccaggagat ggggcggatg ggtccagagg
     3181 aatggaggcg aagggtccac agggcgcaga gggggcggag agaacagcag cgagagttgg
     3241 gtgttgtccc cgacgatgag atccacccga ccgactacgt gccccgcatc ttgccggcgg
     3301 gggactgggc tgtgttggag cggggtctgg cgcagcggat gctcgctatc aacgagtggc
     3361 tcagacgtct ggaggccgga aaggatgagg tcgtccccca ggaggtcatc gagagcagca
     3421 ccctcttcga cgcttcgatc ccaacccgat tcggtgacgt gccaaaccgg cagatgggct
     3481 tcgacatagt caccgtcgag cgttccggtc ggcccggcga atgggaatac ctggtcatcg
     3541 aggacaacgc caagatgccg gtcggtatcg aaccgatgaa tatcctcagg gccggaaccc
     3601 ccgatgtgct atccgagtcc tacgaggcgc tccgggtgag accgctcgac gggctaatgg
     3661 agcgcttggg cgaagtcgtg aggtccgctt cgcccaagcc cgatccgact ctggcgatcc
     3721 tttcaacagg gcctcatgac cagtactacc tcgaccacaa gatcttcgcc agcgagttag
     3781 gggcgatcct ggccgaacgg cacgaggtgg agctcgaccg cgacggaaga ctggtccaca
     3841 gaaagagcgg ccagcggatc gacgtaatct acgagcgcat cgaggacggc aggatctacg
     3901 acgacataaa gggcctgatc gaatctcagg ccagtggcct catcgacgcc gtcttcgccc
     3961 ccaacctggg cgttgccgac gataagggcg tctatccctt tatccccgag atgatccgga
     4021 cctacctcgg ggaggagccc gtactggaca acgtcgagac ttactcgctc gccgtccagg
     4081 atgaccgccg ctacgtcatg gaccatttcg acgagttggt cgtaaagagt cgcagcggct
     4141 ggggcggtaa aggcgtcctc atcgctcccg aagagactag ggaggacgtc gaggagttcc
     4201 gtaaacaggt cgaagataac cctgtggagt acgtggccca ggagacgata gacttctcca
     4261 cccacgtcct ctgcgagacg gacgaggcca acgatagctt cgttctgcgc gacgcccacg
     4321 ccgactaccg catccacgcc cttgccccgg acgcccagac catcgaggtc attccgggcg
     4381 ccatgacgag ggtggcggca ccaggcagca aactggtcaa catctccagc gggggcaaga
     4441 tgaaggacac ctgggtgttg cgtggtacat gaggcaggcc tcatgcacca cgagcatttc
     4501 agcacgcgcc ctgtcgggcg cttgtcagca tttcagctag tcagccttcg cgcgctacac
     4561 ccacgtcgac gaaagatggt ggcagtgcac ggtgccggcg gcgcttatag cgtggtgagc
     4621 gcgcgcctca tggcatccca ggcgagggtg gcgcacctga tgcggttggg cgtctgaacg
     4681 atacccttga gggcggcgag ctcgccgagc tcttcccttt cctccgtgcg catcatctcg
     4741 aggaaggctt ctatcgtctc ctccacttcc tcgcggctct tgcctgtgag ttgctccgtg
     4801 agcatggagg ccgacgcctg cgagatggag catccgcggc cggtaaagct cacctcttcg
     4861 aagaccccgt catgagccct ggcgtagacc gtcaccacgt cgccgcagag agggttatcg
     4921 atgtgttcgt ggatgtcggc atcctccagc tcgcgccggt tgcgtggatg ctcgaagtgg
     4981 tcgagtagga tctctctgta gaagctctgc atcaccttgc gggagattgt atcaggagcg
     5041 cgcggcgctc ccggaaacgt atcgctccgc ggtggcggga gccttcgagc agcatgctcg
     5101 gctacctcct gcggcgtctg gtcccggtta ctttagcttc cgccagagcc acgtagatat
     5161 catcgccctc gaccttgacc tcgtgtttgg ggacgggtct tatggccggt agggtgagcg
     5221 ctttcccggt ggtcacgtcg aacttcgcgc cgtggcgcgg acacttgatc tggttgccga
     5281 tcaactctcc ctggtccagg ggaccggcgt cgtgggtgca cacgtcctct atagcgtaga
     5341 actcgccgtc cacgttgcag agcgccaccc ggtggccctc cagttcgtag acgcccacct
     5401 cgccgggagc cacgtcggcg attctggcta tcttgtggaa ctcagccatc cggttaagct
     5461 acacgctcct cgaagccgag cccgatcttg ccctctatag cgcggtcgag cttgtctttc
     5521 aagcccttca gtgggatcct gctcgtaacc tcgccgaaga aaccgaacac gacgagcttc
     5581 tcggcctcgc ggcgtgggat cccgcggctc atgagataga agagctcgac atcgtccacc
     5641 tggccggtgg tggagccgtg ggagcatttc acgtcgtcgg cctgaatctc cagccgtggc
     5701 agggagaccg ccatcgcatc atcggtaccg aggatgaggt tgcggttggt ctggtaggcg
     5761 tccgtcttct gcgcgccggg ctcgacccgg atgaggccgc tgaataccgc cagcgactcg
     5821 tcgcgcaagg cgcccttgta aaggaggtca gagtgcgcgt tcggggcgag gtggtcctgc
     5881 aaagtgtgat gatccaggac ctggttggag tccgcgaagt acaggccgag catctcggag
     5941 tcgctgcccg cttcggccag gcccgcgtcc acgttggtgc ggctaagctc ggaacccagg
     6001 gccaccacga ggctgtttat ggtggcgtcc ctgcacgcct cgctgcggat ggtgttgaag
     6061 tgcagcacgt tgcggcccca gttctgcaag gagacgtagc gcaggtttgc gccctgcccg
     6121 acgtagagct cgaccgcccc gttgctcagg gcgggctcgt cctcctcggt cccgtccgag
     6181 gcatactcgt ctatgtaggt gacgctagca ttttcttcga cgatgacgag tgtgcgcggc
     6241 atcgtggcgc cgatcgcgtc gagccaacgg tagctctgga tcggcacctc cacgtctacc
     6301 cctcgcggca cgtacaagaa cgacccgccc gcgaacagcg ccgccgatag cgccgagaac
     6361 ttgtcgtagt cctcaggcac cagccggaag agcttttctt gtatcaggtc ttcgtgctct
     6421 cggagcgctg tgtgcaggtc ggtgaagacc acgcccttgt tctgcagctc gtcgtccatc
     6481 cgctggtacg tggtctcgga gttgtgctgc acgagcagag cagagttctc ctcgccctcg
     6541 cggatgagtt tctggacggc gtcgggcagg tcgccttcgc tcgatacgtc cgggctcggg
     6601 acgtaggggc tgaaatcctc cgggttcacg tccgagatgt ccgtatagcg ccacgcctcg
     6661 tcgcgcagcg tgggcatcgg caacgcctcg aaggcgcgcc acgcgcccaa ccgcttctcc
     6721 gtgagccagc caggctcgtc cttgaacgag gagagggcct tcaccgtctc ctcgctcatc
     6781 ctgctaccct tcagaacctc cggcgcttcc ttctgcatgc gacctacctt tgatctctaa
     6841 gcatagggac ggactccgaa gagagcccgt cccacaaaac ctacctgaca agctgaaatg
     6901 ctgactagct gaccagctct accccaccga gccttccatc tccagttgga tcaggcggtt
     6961 cagctcgata gcgtactcca ggggaagctc cttggctatc ggctcgataa agccgtttac
     7021 aaccatcgcg atggcctctt cctcggagag ccctcggctc atgaggtaga agagctggtc
     7081 ctcaccgacc ttcgagaccg tggcctcgtg ctcggtgttg accttcttct cctcgatctc
     7141 gatgtacgga tacgtgtccg tccgcgcgtt ctcgtccagc aagagcgcgt cgcactcgac
     7201 ccggctcttg gagtcgtggc agccctcatg aaccttcagg agcccgcggt aggtgctccg
     7261 tcccgtcccc ttggagatgg acttggaggt gatcaggctc gaagtgttcg gcgcggcgtg
     7321 gacgaccttg ccgcccgcgt cctggtgctg tccgtcgccg gcgtaggcca cggagaggat
     7381 ctctccgcgc gcgccctcgc cgaccaggta caccgccggg tacttcatgg tcagcttgga
     7441 gcccaggttg ccgtccaccc actcgatggt ggcgttctcg tgggccacgc cccgcttggt
     7501 caccaggttg taggtgttgt gcgaccagtt ctggatggtc gagtaacgga tgcgcgcccc
     7561 cggcttggcg atgagttcga ccaccgccga gtgcagcgag ttcgtggtgt acgtcggggc
     7621 cgtgcagccc tcgatgtagt ggacgtagga gtcctcatcc gcgatgatga gcgtccgctc
     7681 gaactggccc atgttctccg cgttgatgcg gaagtacgcc tgcagcggga tgtcgatgct
     7741 gacccccggt gggacgtaca cgaacgagcc gcccgaccac accgccgagt tgagcgccgc
     7801 gaacttgttg tcatccggcg ggatgacggt cccgaagtac tccttgacta tgtcctcgtg
     7861 ctcgcgtagg ccgccatcca tgtccaggaa gatgacgcct tgctcttcga gttccttctt
     7921 cagcgagtgg tagacgacct cggactcgta ctgcgccccg acgccggcca ggctctccct
     7981 ctctgcctgc gggatgccga gtttctcgaa agtgttcttt atgtcgtcgg gcacatcgtc
     8041 ccaggagcgg ccctggttct ccatgggcct gatgtagtaa tagatgtcgt cgtagtccat
     8101 cccggagagg tcgccgcccc aggtgggggt cggcttctcc aggaacgact ccagcgcgtt
     8161 cagccggaac tccagcatcc actccggctc gttcttgtgc ttggagatca tggccacgat
     8221 ctccgggtcg atgcccttcc tcggggtcct gaagaagtac tcctcaggat cgcggaaacc
     8281 gtacttgtac tcgtcgaggc cgagctcttg tatagtcttg tcttctggca ttccttcgtc
     8341 ccttccctag ctcctgctag cttcgctagc tccggcgccg aactcctggc gcacccagtc
     8401 gtagcccttc tcctccaatt cctcggcgag cttcttgccg cccgaggtta cgagcctgcc
     8461 atcgagcatg acgtggacgt gatccggctc gatgtaccgc aggatccgct ggtagtgcgt
     8521 gatgataacc gccgagaact cgggcccacg cagctcgttg acgcccttcg caacgacctg
     8581 caaggcatct atgtccagcc ccgagtcggt ctcgtccatg atggcgagtt tggggtcgag
     8641 catgagcatc tgcaaaatct cgttccgctt tttctcaccg ccggagaagc cctcgttgag
     8701 gtacctctcg gcgaacttgg ggtccatctg catgatgccc atcttctcct gcaggagcct
     8761 gtacatctcc atgggagtga gctcctcctc gcgcacggag ttgaccgcgg tcctgagaaa
     8821 gttggccacc gagacgccag ggacctcgct cggatactgg aaagcgagga acatcccgag
     8881 cttggcccgc tcgtcgggct ccaactcgaa gacgtcctca tcctggaagg tgatcgaacc
     8941 cccagtcacc tcgtaacgag gatggcccat gagcacgttg gcgagcgtgc tcttccccga
     9001 gccgttagga cccatgatcg cgtgtatctc acccttgcca acctcgagat ccagaccctt
     9061 cactatctcc tggccgtcga tctctacgtg aagatcttct atcttgagca tcaaacccct
     9121 gccgttaggc tatgtaagaa ctttgctaca gcaaggctat taaatcacag caccaggggc
     9181 ctaacaacgt aaagagttca cgaccaagaa ctcttcttga aacgtccgaa acccgactgt
     9241 ttataaggat ttcgtgatcc aggtcacgtt ctgcgaaggc ttctcgggaa ccactgtcag
     9301 aacgtcctgc tgccggccgc ttgtaacagc gcacagcgct cctaaacggt ggttattgag
     9361 aacgtttctc gataagacgt ccgggagttt gacactatcg ctacgttttt cgcgactgtt
     9421 gcatctcgcg gtcttcgggg cgcttcatgc cggctctaca ccgagcaccc ttgcgtcctg
     9481 ggcgttcatg ggtgcttcga ggtcggcttt gcgggccagg tagccgagcg tgtatatctt
     9541 cccttccacc ggtaaggggg caaccgaaga gagccggccg acgacatcag ccgaggagac
     9601 tcggccaacc agcttccttt cgccctgcaa gatctcatcc cccgcctcgg gcggctccgt
     9661 cgagccttgc tccagttcga agcggtagag cctcttgttc gggctgccac ggtaatgcat
     9721 cctggccacc gtctcctggc cagggtagca acccttttcg aagctgatgg cccgctttag
     9781 agagttcttc gactcgcccg ggaagttctc gggtgtgatg tccgcgccga agcgggggac
     9841 gccggcttcg atgcgggcgg tctcgtaggc gtcggcgctc acggtggtag cgccggtctc
     9901 gttcaggtat tcgcggaccg ctgcgaggtc ctccgggggc ccgatcaggt cgtatccggg
     9961 aacgggaaac gcgacgccaa ccgagagcaa gatccggcct tcgatctcta tctctctaga
    10021 ttggtgttcc tcgagatccc caaagtactc tctggcgcgg ggaccataga ggccgagtac
    10081 ggaccacgag agctcctcca gtctcacgcg ggagaaagga gcgtagcggg ttaggatctc
    10141 cttcgccgcc ttggcgccct ccggttccgt gtcgatgagg atctcgtcgc cggccttgac
    10201 gacacggaga tccgtttgga tccggccctt cggatcgagg agcatggcgt aggcgccgcg
    10261 gtcttcctgt gtcggcacgt cgctggtcag gacggcgttg agcatcccga tggggtcctt
    10321 gcccgtgaga cgcaggagcc ttctatccga gcggtccacg atcgcggcgc catctctcag
    10381 ggctgcgtag ccttcgaggt cgtttgggtc ggcgttgttt tggggatctt tcgaatactc
    10441 catgagtcta ttctaaggca cgagaggagc ctggatggtg aaagtggtcg tactggacgt
    10501 tgacggcacg ttgatggaca cgaactacct gcacaccgag gcgtgggcgc gggcattcga
    10561 gggtgccggc catcgggtgc cgagggtcag gctccacaag caggtcggta aaggcgcggg
    10621 gctcttgatc cgggagttcg tcgacgacga ggagaaggta gagaagatcc agaacctcca
    10681 tagcgaggtc tacgcagaac tgcaagaata cggccacccg ctcccgggag ccaaggagtt
    10741 gatctcctcg ctcaaagaac gcggctatga agtctggctg gtcaccagcg ccaaagacga
    10801 agagctggaa caccacatgc aagagctcga agccggagag agcgtggacg gcgtcgtcaa
    10861 ctcgagtgcc gtcgagaacc cgaaaccggc acccgatatc ttcgaggaga ctttgcggag
    10921 ggccgggtcg accgccgacg agaccgtcgc cgtcggtgac gcggtgtggg acgtcgaagc
    10981 cgccagagaa gccggcatac gcaccgtcgc cgtcctctcc ggcggcgcct accacagaga
    11041 cgacctcgaa gaggccggag cggtggccgt ctacgaagat tgcgccgcac tcctcgactc
    11101 cgacttccct gagtagcgcc gggtccgcgt gaaagcctgg gggttttgta ctagagtgcc
    11161 acacgcaagc tggaagaggg actggttgtt ggggggcata ccccaaggtc tgtcatccaa
    11221 agatcgggct cgagaagaga aaggtgggcg caaagggtat ggcccaggga acggtaaagt
    11281 ggttcagcga cgagaagggc tacggcttca tctccccgag cgacggtggc gaggaccttt
    11341 tcgtccacta cacggggata gagggtagcg ggttcaagac gctcgaagaa ggcgccctcg
    11401 tgaccttcga gacctctcag ggaaagaagg gacttcaagc ggtaaacgtt tcgcccgcct
    11461 gaaattgcag gcatcaggtt tcaggctaca ggcatcaggt ctcgccggtg ggtgtctgca
    11521 cgttcggccg tacctcgaag gaagtacgat agggctgttg aggggagtgg cgatggctag
    11581 cttccggtag cgatcaatct ggttcctcct gaaacctgaa acctgatgcc tgtagcctga
    11641 tctcaaaagc ggtcctcgca tcctcgtaca ccctgggtag tgggagcccg gtctctttgg
    11701 cgagcctggc ggcgtcggcg tactcggggg cggcgacgaa gtcttcacct tcgatgctac
    11761 ccaccttgac cccgcacctg ccgtagggaa ggtctaccac gacgcggcgg cgtcccgcga
    11821 cggtgcggcc gaccgtgtgg tggcgcacgc cgagggttcc ggtctcccgc atgaggagcc
    11881 atgcgagacg ctcgcggtcg ccggtgaggg ccagggcgca aaccttgtaa gcgccgcggc
    11941 cacgcttcat cacgatgggt tcgagccagg cgtctagggc gccggccgcg agcagacgct
    12001 ccaccgcggc cccgaggatc tcgcccggag cgtcatcgac gttggcctcg agtacttcga
    12061 tggcacccac aggcccttcg agctcaccga cgacgacgcg cagcaggttg ggcgcgctcc
    12121 tcaggcgtgc ccttccggtc ccgtagcccg tggcgtggag ggtcatgggc ggggccgcgt
    12181 ccgtaaactc gccgcccgtg aaggatctca tgagcgcggc acccgttggg gtggtcgtct
    12241 cgacgggttc gtcggtccag cggacgggag atccctcgag gatctcgagc gtcgcgggtc
    12301 cgggcacagg caggggaccg tgggcggcat cgacgacgcc cgtgcccatg ggcagaggac
    12361 cgcagcttac ggaggcggcg ccgagcagtt ccagcgccac gcagctaccg actatgtcga
    12421 ctatcgagtc cacggccccg acctcgtgga aggtgacctc ttcggggtct tcgtcgtgga
    12481 cggcgccttc cgcgacggcc aaaaggcgga aagcctccag ggcgcgtgag gaggctctct
    12541 ccggcaatgc ggcgccctct atgcaggcgc ggatatcccc gaaggtcctg tgggggtgct
    12601 ccccagggta tccgacgtct gccctgagcg tgcctatacc gtttacctcg acacgctgca
    12661 cagcgaggtc gaagcttacg ccgaggccgc gcaggagggc tgtgatctcg ggcagcggcg
    12721 ctcccgccgc gacgagcgcg gcgagggtca tgtcgccggc cgcgcctccg atgggttgga
    12781 aatgaacgtg tttcaaaacc ggtctcccgg cttcgtggag gccggcctgc gatggatggg
    12841 agtattcggg tccgggggaa ccgcccctct attcctctcg tccgttggag atcttgcgca
    12901 cggccataag ggtgacggcc aggctgatgg caagcacgat caggggcctg gggtcgcgct
    12961 tgacggcgtc ggtcaagggg gcggggttcc tcttcctgcc ggcctccccg atcatcttcg
    13021 cctggcgact ggccgattcc ttgaggttct tgccgaagcg gattacggcg tcccttacgc
    13081 gttctttcac cttcgtagag gtccgcttca ctataacctg gggctcggcg tgctttttga
    13141 ggtccctgac gtccggcgcc atgcggctcc ggatctcgaa catctccctc tctatgcgct
    13201 cgggtatctc gttcattacc tcaactgccc ctttatccag ttgatgttct gctgaagggt
    13261 acggatagtc cggtggggcg tgggatccag tctcctgagt atgccggctc cggccgcgcc
    13321 tagtatcccc ccgaccagca gcaatacgcc actgacgatc agagccgaga cccagaggga
    13381 gaggaagacg tcgagtaggt aaaccccggc aagggagaag attatgagga agaccaacat
    13441 gaagaccccg gcggccgcga ggaggccaga agccaacccg cccctcttgg ctatgggcgt
    13501 catctccgtc ttcgccaact cgagctgctt gtcgacgagc tcctgaagct ggggcctcag
    13561 agcgtcgacg atctccttga tgctgcggtc cgaaggctcg ggaccctccc tgagctccct
    13621 gccgaactct gctacccgct ctcgcccgtc catccccacg ctcctttctt ctgatcctag
    13681 aactcggagt atctcttgct tacgctccta gttaccctcg cctcgccctg cgtaatccgg
    13741 caggcctcct ggggacgacc atccctatcc tacaccagat cctagtatcc aacatgccat
    13801 aaagcacgcg aagtgaatcg atgatgtaac atgaatacag atgtacaaga cccgtcgttc
    13861 cccggctgga tgggagtggt attgaagttg catgtagcgc tagggatctc ctctggagac
    13921 gtggccgcct tcgtcggggc ggggggcaag tccggtgcga tccttgctgt ctcgggcggg
    13981 ctcacggagg agggtatgaa ggtccttgtg gccccgacga cgaagatgtt gacgagcgag
    14041 gccgacagta taggtccgct cgtcacctcg gaagacgccg gcgacctgcg cgcaaggacc
    14101 gagagggcac tatcctcggc ccctgcggtg gtcgtgggat cggggatgct ctccaagaac
    14161 agggtaggcg gcgtggatcc cgagtgggtg ggcgatctcg ccgggctcgc cgacgttgtc
    14221 ctggtc
//
LOCUS       DATMVF010000023        13363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_415348, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000023 DATMVF010000000
VERSION     DATMVF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13363
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415348"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VN178_00870"
     CDS             <1..1217
                     /locus_tag="VN178_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79543.1"
                     /translation="MGRAGDLYGRRRLFVGGLALFAAASFLGGLAWAPWVLVLARLLQ
                     GVGGAALVPASLALVSATFAAGEARNRAMGVYGAMAGVGFVFGMVLGGMITEFLGWRW
                     VLFVNVPVAIGVLSLAPVVIHESKDEAVPRTLDPAGAATATLGIAALIYAISEAPKHG
                     WASPATLVTAGLGAVLLGAFVAHERRASAPLVRLQIISRRAVAIPNAAIVLKSMVGAA
                     QLYVLTLYFQDALGRTPLEAGLLFIPMTLASVVASPVAGGLTSRLGTRRTAAWGFAIS
                     GAGLVLVAWLMPHEGALVAVLAGMAVAEAGFVTTSVPLTVAATDGVGDDERGLASGIL
                     STSTEFGNALGFSAVAAVIAATTASDSATLLGGLRWGLWSATAYAALAVLLVVVCMRP
                     YSREAKGFSSNP"
     gene            complement(1224..1592)
                     /locus_tag="VN178_00875"
     CDS             complement(1224..1592)
                     /locus_tag="VN178_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HWS79544.1"
                     /translation="MRRIILLMTVATLVVVSTFFVVSASGAHKHPAAKAQNKPTKTVV
                     IQNFRFKPANITVKRGTKVRWINKDSTAHTATANNGRTFDSGLLRKGQRYTHTFKSTG
                     KKRYHCEPHPHMRGTVVVKR"
     gene            2005..2799
                     /locus_tag="VN178_00880"
     CDS             2005..2799
                     /locus_tag="VN178_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HWS79545.1"
                     /translation="MGFDAICQHLEFEAMDRIVSPGQQPPVGHLHDHYGTLATPDATV
                     SQMQQSTSACNRPEDTAGYWVTALMKPDGTPVPATAMKAYYRTAVPASKVKPIPLGLM
                     MVAGNHMATAANPQSTSVVQWNCKNQVTGEVTGPWVEPHQCESPDAKPRLKIVFPQCW
                     DGKRLDSPDHKAHTAYPKRVNGVYKCPSTHPVAITRLGLQFNFPAEGDPGWADMDTVT
                     FSAGGRFSGHADFWHTWRVTGENGFNSLHTRCLNEGTYCGLGNQPR"
     gene            3159..4043
                     /locus_tag="VN178_00885"
     CDS             3159..4043
                     /locus_tag="VN178_00885"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HWS79546.1"
                     /translation="MLIMGVASVAACVAAFMWWKAPDASAQVAASPEAVFVTRCNYSH
                     TNSDDPIVHAGKPGAAHSHDFFANRSTDHASTYEGLRTYTDTTSATRSGTTCVNAADK
                     SAYWIPTTSWKDKNGTRTLKPGRAHFYYRSGGKGDVQPPPAELKVVPNTHVTWRCAQA
                     TSASSSPPTRCSNGTLVVLIRFPDCVAEDSNGQPIIDSADHRSHMAYAVSQSDGLRRC
                     PDTHPYPVATLLMEVKFRVPITAGTVTLSSGAASTMHADFFNAWDQDELTALVDGCIN
                     AYPFSPENPKPAKCEQRG"
     gene            complement(4118..5050)
                     /locus_tag="VN178_00890"
     CDS             complement(4118..5050)
                     /locus_tag="VN178_00890"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWS79547.1"
                     /translation="MVGRNQKAGVDLAVELCGLRLRTPLIPASGTLSKEALGEVGGVY
                     GAMLPKTTTPAARAGNPPPRIAETPAGMVNSIGLQNPGVEAFLQGLDAFDLGIPIFVS
                     VAGDTIEEFSQLCGTLAGEARVSAVELNLSCPNVECGGLAFCAGPASVEEVVAACRAA
                     VPGKPIIAKLTNEGVLENARAAESAGVDGLTVINTLPALTIDVRTRKVLVRGGLSGPA
                     IKPVALRAVYDVSGAVNIPVIGSGGVVSGTDVAEFMLAGATAVQVGTASFVRDPQEIL
                     DEFTDYLKENSLNAAGLTGAIRGGRPVLAGGRDI"
     gene            complement(5044..5778)
                     /locus_tag="VN178_00895"
     CDS             complement(5044..5778)
                     /locus_tag="VN178_00895"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HWS79548.1"
                     /translation="MKFYSVEVEQREELGPYTLLEYRWSLGPVRPGQFVMARAAGYPA
                     TLDPFLARPFSFYDYDGKMASLLFEVRGRGTALLDLASTMEVTAPLGNGFRIDPGLGR
                     AALLGGGIGVAPLKILSRSLDAAGIPHDVFLGFADASPAAVAADFPGAGVATMDGSAG
                     TPGTVLDAADSLEVYGAVYACGPNPMLAAVKRAARESRLCQLSVEERMGCGNGSCNGC
                     VVPVRGGDYIRSCIEGPVFHADVLAW"
     gene            complement(5775..8939)
                     /gene="carB"
                     /locus_tag="VN178_00900"
     CDS             complement(5775..8939)
                     /gene="carB"
                     /locus_tag="VN178_00900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWS79549.1"
                     /translation="MPRRNDIESVLIIGSGPIVIGQAAEFDYSGTQACHALREEGYRV
                     ILVNSNPATIMTDPEVADVTYVEPLTAETVAEILRRERPDALLPTLGGQTALNLAVEL
                     NESGVLDETGVELLGASIPSIRKAEDRKLFSQAMRRIGLNVPPSRTVRSIAEAEELAA
                     QIGFPLIIRPSFTLGGKGGATVTDHRELRRAVIDGLEASPVGSALVEKSVAGWKEFEL
                     EVMRDLADNVVVICSIENVDPMGVHTGDSITVAPAQTLSDRQYQTLRSASLRIIREIG
                     VSTGGSNIQFAVDPESEAYYVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYSLDE
                     IPNDITRATPASFEPALDYVVTKVPRFAFEKFPGAAPRLTTRMHSVGEVMAIGRTFTE
                     SLMKAMASLEVDAQDVHAGLDEPSPYRVFAVFDALRAGMDIMEIFERTSIDPFFIASI
                     ARIVAAEGAVSEPFGVEDLRELKRIGLTDETIATASGSSVEVVRGVRRALGVSPTYKS
                     VDTCAGEFPARTPYYYSTYEEEDEVRREENASVVVLGSGPNRIGQGIEFDYACVHASY
                     ALAQSGYDAVMVNSNPETVSTDYDTSTRLYFEPLTAEHVLEVIKREEPEGVILQFGGQ
                     SPLKLAHELEQAGARILGTSPDAIDLAEDRSRFGRLLADLGIPHPRFGSASTAEEARE
                     VARELGYPVVVRPSYVLGGRRMEIVYNDEDLDLYLRTSAGAGPGHPTLIDKFMEDYTE
                     VDVDAVSDGEEVYVGGIMEHVEEAGVHSGDSSCVTPPINIHRGLLQRIEDYTRWLALS
                     VGVVGLMNVQFVIRGDDVLVIECNPRASRTVPFISKATGVPLAKIATRVLLGEKLRDM
                     ALRASTNGHFSVKAPVFPFDRFADVDPLLGPEMRSTGEAMGIDRTFGGAFAKALTAAG
                     QELPLSGRVYISVANREKRAVVLIARAFADLGFEISASEGTAEVLKNNGLPVMIVPKI
                     GELGEDVLSLIEAGGVDLIVNTPWGRGPRTDGYLIRRRALMHGVPCITTLAGAAAAVQ
                     GIEARVRGGTRRVNSLQGLYAARA"
     gene            complement(8941..10050)
                     /gene="carA"
                     /locus_tag="VN178_00905"
     CDS             complement(8941..10050)
                     /gene="carA"
                     /locus_tag="VN178_00905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWS79550.1"
                     /translation="MRNGGRSRATLVLEDGAAFRGWSFAGHGEVAGEVVFTTSMVGYQ
                     ETITDPSYRGQILLFTYPLIGNYGVIPGDDESRRVQAAAVLVREYTPHPSNWASERSL
                     AALLEECGVLGVEGIDTRALTRHLRDRGAMRGVISTSESDRNVLRERAIAHPSMVGLD
                     LASTSSELTEPTLLPAFGEERCCVVALDYGVKGSIYRELRSRGTSVLAMPGSATSEEI
                     LATEPDGLFLSNGPGDPAALGAAVDTLRPLLGEVPTFGICLGHQLLGLALGCETYKLP
                     FGHHGANHPVRNLDTGRIEITSQNHGFAVSEDSLAGGVELTHRNLYDGTVEGISSPHQ
                     RAWSVQYHPESSPGPRDSGYLFDQFVNSISEKARA"
     gene            complement(10043..11350)
                     /locus_tag="VN178_00910"
     CDS             complement(10043..11350)
                     /locus_tag="VN178_00910"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWS79551.1"
                     /translation="MLRGGYPAELVIRHAHVLDPATGLDGILDVRLSSGRVAEIGEDL
                     RGPRTLDADGLHLFPGFVDVHAHWRTPGREDEEDIESGSAAAAAGGFTCVVMMPNTDP
                     IVDRPVVVSGLVRRVERESRVRARVAAALHVGLAGERLTEMRLLKQAGAFCVSDDGLG
                     TASAGVLRNGMLYARSAGLPVILHCEDHTLATGVVHDGVAASLAGIPGSPASAEDAAT
                     ATALILAAETGARVHITHVSTALSAALVGFFRDRAEITADTTPHHLTLTEDLVSTLDG
                     LYRVNPPLRPEHDRAGLGPALRDRVLDFVATDHAPHAPEEKELPIEEAAPGFLGHETA
                     FAALYTELVLGGALSLKRLVEAMGSGPGRWVGTGGTLAVGAPADLTLVDLAEEWTVGS
                     ATLVSRSSNSPYLGKTLKGRVVGTIVGGEPVHDRTGAKFGARA"
     gene            complement(11350..12267)
                     /locus_tag="VN178_00915"
     CDS             complement(11350..12267)
                     /locus_tag="VN178_00915"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWS79552.1"
                     /translation="METRSLLTLEGIGREDIREILDLARAFAEGRLRGALEGKTVCLA
                     FFEASTRTAVSFELAARRSGAHVISLSEKGSAIAKGESLIDTVVTLDRLGADAIVLRH
                     RAAGAAALAARHTSAPVINAGDGCGQHPTQALLDLYALGEALGGFDGLQDRRVAVVGD
                     VLHSRVARSVIPGFLQAGMEVALVAPRTLLPPDEGVFDLPVLASVDEALEWGADVLYM
                     LRLQRERMTGARIPSVAEYARFYGVSRRHLEGGALIMHPGPVNRGVEISGDVVLDASS
                     LIPDQVAAGVCVRSAVLALSTGAVQSVAA"
     gene            complement(12257..12805)
                     /gene="pyrR"
                     /locus_tag="VN178_00920"
     CDS             complement(12257..12805)
                     /gene="pyrR"
                     /locus_tag="VN178_00920"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWS79553.1"
                     /translation="MGRTSVYAHSKVLAPDGLNRSLRRISHEILERNASCLDRLALVG
                     ILTRGVPLAQRISANVREFEGLELPVGSLDITLHRDDLEKKDPEVRGSNVPFDVTGKT
                     VILVDDVLYTGRTARAAMDALLELGRPAAVRLAILVDRGHRELPIRADYVGKNVPTAR
                     DERVLVNLEETDGEDGVIIVGD"
     gene            complement(12865..>13363)
                     /locus_tag="VN178_00925"
     CDS             complement(12865..>13363)
                     /locus_tag="VN178_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWS79554.1"
                     /translation="RDTSGLMVLAKGEPAYSRLVGMLSARRVGRLYRALVGGEGLPRT
                     GTIDSPVGRDPDNPTLMAAGVGKPAVTHFEVLATAAEHTMLRVRLETGRTHQIRVHLS
                     AIGYPIYADPLYGDPVPGHRLWLHAERLSFEHPVSGEALVFRSEIPEDLRDSAVGLDG
                     SLALW"
BASE COUNT         2268 a         4311 c         4387 g         2397 t
ORIGIN      
        1 tcatgggccg tgccggcgac ctttacggcc ggcggcgcct gttcgtgggt gggctagcct
       61 tgttcgccgc cgcctcgttc ttgggcggtc tggcgtgggc gccctgggtg ctggtgttgg
      121 cacgcctatt gcagggggtt ggcggggcgg cactcgtgcc ggcttcgctt gcgctcgtct
      181 cggccacctt cgccgcgggc gaggcgcgta atcgcgccat gggcgtctac ggggcgatgg
      241 ccggggttgg gttcgtcttc gggatggtgc ttggggggat gatcacggag tttttgggct
      301 ggcggtgggt gttgttcgtg aacgtgcccg tggcgattgg ggtgctctcg ctcgcgccgg
      361 ttgtgatcca cgagagcaag gacgaagccg tcccgcgtac cctggacccg gcaggggcgg
      421 cgacggccac cttagggata gccgcgctga tctacgccat ctccgaggcg ccgaagcatg
      481 gctgggcctc ccccgccaca ctcgtcacgg ccgggctcgg ggcggtgctc ctcggagctt
      541 tcgtggccca cgagcgccgg gcgtcggcgc cgctagtaag gctgcagatc atcagccggc
      601 gtgccgtagc catcccgaac gcggcgatcg tcctcaagtc catggtgggc gctgcccagc
      661 tctacgtgct gacgctgtac tttcaagacg ctcttggacg cacgccgctc gaggccgggc
      721 tcctcttcat acccatgacc ctcgcttccg tcgttgcctc gcctgttgcc ggagggctca
      781 cgagcaggct tggcactaga cggacagcgg cctggggatt tgccatctcg ggggccgggt
      841 tggtgctggt ggcgtggcta atgccccacg aaggtgccct ggtggccgtg ctagccggga
      901 tggcggtggc agaggccggc ttcgtgacaa cgagcgtccc cctgacggtc gccgctacgg
      961 atggcgtggg ggatgatgaa cggggcctgg cttcagggat tcttagcacc tccacggagt
     1021 tcggcaacgc cctcgggttc tccgctgtcg cggccgtgat cgccgctact acggcgtcgg
     1081 attccgccac cctactcggg ggtctccgat gggggctctg gtctgccacg gcctacgccg
     1141 cgctggccgt cctgctcgtg gtcgtttgca tgcgtcccta ctcgcgagag gcaaaggggt
     1201 tctcgtctaa cccttagcta tcgttaccgc tttacgacta cggttcccct catgtgggga
     1261 tgtggctcgc aatggtatct tttcttcccc gtgctcttga aagtgtgcgt atacctctgt
     1321 cccttcctca acagcccgga gtcgaaagtt ctgccgttgt tggccgtggc ggtgtgggct
     1381 gtgctgtcct tgttgatcca tctgaccttc gtcccacgct tgacggtgat gttggccggc
     1441 ttgaagcgga agttctggat cacgaccgtc ttggtaggct tgttctgtgc cttggccgcg
     1501 ggatgcttgt gcgccccgga agcggaaacg acgaaaaagg tgctcaccac gaccagcgtg
     1561 gccaccgtca tcaacaagat gatgcgcctc atcagcctta cctcctcctt ctcccgcccc
     1621 acagcggaga tttgtgcata acagtagtac gcccgggcag gcaacacaac ccctaacgac
     1681 cgtttttctg gtgctgttct tgcttttgtg agctgccgct agatgcatgt ccggctcact
     1741 cacccgagtg cccgggcggt gcattctccc tgattcatat ccagcacagc ctcggacgga
     1801 ccgggcaaca agtgcgtgtc ttgcggtgtc gattcgcgaa tcaggtggcg gggggagtct
     1861 cgattcagta tattggagcg tgcgttccaa aggagagggg tcgatatgcg acgcttgttg
     1921 ttattgctac tagttctcgt agtgggggcg tgtgccccgg aggaacaaga cgtaagtctc
     1981 agcgccacac tcgaggaacg ccggatgggc tttgacgcaa tctgccagca tctcgagttc
     2041 gaggcgatgg accggatcgt gtcacccggt cagcagccgc cggtgggcca cctgcacgat
     2101 cattacggca cgctcgcgac acccgacgcg accgtttctc agatgcagca atccaccagc
     2161 gcttgcaaca ggcccgaaga cacggccggc tactgggtga ctgcgcttat gaaacccgac
     2221 ggaacaccgg tccccgcgac ggccatgaaa gcttactacc gcaccgcagt accagccagc
     2281 aaggtcaaac caatcccgct gggcctaatg atggtcgcag gtaaccacat ggccacggcg
     2341 gccaaccctc agtccaccag cgtcgtgcaa tggaactgta agaaccaagt gacaggcgag
     2401 gtcacagggc cttgggtgga gccgcaccaa tgtgaatccc ctgatgctaa gccgcgcctg
     2461 aaaatagtgt tcccacaatg ttgggatggc aaaaggttag actccccgga ccacaaggcg
     2521 cacaccgcct accccaaacg tgtcaatgga gtgtacaaat gcccaagcac acaccctgtg
     2581 gccatcacgc gcctcgggtt gcaattcaac tttcctgctg aaggtgatcc cggatgggct
     2641 gacatggata ccgtcacatt cagcgcgggt gggcgtttct ctggtcatgc agacttctgg
     2701 cacacctggc gcgtgacggg agagaacggg ttcaatagtc tccacacccg gtgcctgaac
     2761 gagggtacct actgcggtct tggcaaccag ccgcgttaag ccaggtgcag gcgacggccc
     2821 tgacggcatc gtccttgcgg cgattgaaga ggtggtctag gaagctacca acaagtacga
     2881 atctgcaacg ccaccgcggt cgctctcgaa gaacgtctgc aagcactcga gagctgcgct
     2941 ggtgaaacgt gccgccgacc gagggtaaac agtagagcag cttgcggggg atcacgggtc
     3001 ggttaggagt ggggaccgag ccctgacgtg ggagagaggt gcgatccatg aacctgtgct
     3061 gaggtggttt cgcaaagcag gcgtactgtc gtccatgaac gggggaagga aagatggaag
     3121 caagcagcca acgaacccgc caaggccgct tggcaatcgt gttgatcatg ggggtggcgt
     3181 cagtggccgc ctgcgtggcg gcgtttatgt ggtggaaggc acccgatgcc tccgcccagg
     3241 tggccgcctc ccccgaggcg gtctttgtca cccgctgcaa ctatagtcac accaactccg
     3301 acgaccccat agtccacgcc ggtaagcccg gtgcggccca cagccacgac ttcttcgcaa
     3361 accgctccac tgaccacgcg tccacctatg agggtctgcg cacgtatacg gacaccacct
     3421 ccgctaccag gtcaggtact acttgcgtca atgccgctga caaatcggcc tactggatcc
     3481 ctaccacgag ctggaaggac aagaacggca ctcgtaccct caaacccggc cgcgcccact
     3541 tctactatcg gtcgggcggc aagggggatg tacaacctcc cccggcagaa ttgaaggtgg
     3601 tccccaacac gcacgtgacg tggaggtgcg cgcaggccac gtccgcttca tcgtccccgc
     3661 cgacgcggtg ctccaacggt acgctcgttg tgctcatccg cttccctgac tgcgtcgctg
     3721 aagactcgaa cgggcaaccg atcatcgaca gcgccgatca ccgctcgcac atggcctacg
     3781 ccgtcagcca aagcgacggc ttacgacggt gccccgacac tcatccatac cccgtggcga
     3841 cgctcctcat ggaagtcaag ttccgagtcc ctatcactgc aggtacggtg acgctctcgt
     3901 cgggcgcggc ctctaccatg cacgcagact tcttcaacgc ctgggaccag gacgagctga
     3961 ccgcactggt agacggctgc atcaacgcat atccgttctc gcctgagaac ccgaagcccg
     4021 cgaagtgcga gcaaaggggg tgacggttag cgggttcggt gcatcgttag ggtcgggact
     4081 tctggaagtc ccggcccact cttggttgtt cgacccacta tatgtcacgg cctcccgcca
     4141 gcacgggacg cccgccgcga atggctccgg tcagcccggc ggcgttcagg ctgttctctt
     4201 tcaggtaatc cgtaaactcg tccaggatct cttgcgggtc cctgacgaag ctcgccgtgc
     4261 ctacctgaac cgccgtcgcg ccggcgagca tgaactcggc cacatccgtt ccgctcacca
     4321 cgcccccgct accgatcacc ggtatgttca ccgcgcctga cacgtcgtag acggcgcgga
     4381 gcgcgacggg ctttattgcg gggcccgaga ggccgccccg aaccagaacc ttgcgggtac
     4441 ggacgtctat ggtgagcgcg gggagagtat ttatgaccgt cagcccgtcc accccggccg
     4501 attcggcggc gcgggcgttc tctaggacac cttcgttggt gagcttcgcg ataatgggtt
     4561 tacctgggac ggcggcgcgg caggcggcga cgacttcttc cacggaggcg ggtcccgcgc
     4621 agaaggccag gcccccacac tccacgttcg ggcacgagag gttcagttcg acggcggaga
     4681 cgcgggcttc tccggcgagt gtcccgcaga gttgggagaa ctcctctatg gtatcgccgg
     4741 cgacggagac gaagattggg atgccgaggt cgaacgcgtc aagaccctgc aagaaagctt
     4801 cgactcccgg gttctggagg ccgatagagt tgaccatccc tgccggggtc tcggcgatgc
     4861 ggggcggcgg attgccggcc cttgcagccg gggtcgtcgt cttgggcagc atcgcaccgt
     4921 agacgcctcc gacctcgccc aaggcttctt tggagagtgt tccggaggcc gggatcaggg
     4981 gcgtccgcaa ccggagcccg cagagctcaa ctgcgagatc gacaccggcc ttttgattac
     5041 gccctaccac gccagaacat ccgcatggaa gacgggcccc tctatgcagg acctgatgta
     5101 gtcgcccccg cgcaccggga ccacgcaccc gttgcacgag ccgttcccgc accccatcct
     5161 ctcctccacg gagagctggc agagcctgct ctcccttgcc gcgcgcttga ccgcggccaa
     5221 catggggttg ggaccgcagg catagacagc cccgtacact tccagggagt ctgcggcgtc
     5281 gagcacggtc cctggcgtcc ctgccgaccc gtccatcgtc gccacccccg cgccggggaa
     5341 atctgcggcg acggccgccg gactcgcgtc ggcgaacccg aggaagacgt cgtgaggaat
     5401 ccctgcggcg tcgagactcc ggctaaggat cttgagtggc gcgacgccta taccgcctcc
     5461 cagaagggct gcccgtccaa gccctggatc tatgcggaaa ccattgccga ggggggccgt
     5521 tacctccatg gtcgatgcga ggtccaggag cgcggtaccg cggccccgca cctcgaagag
     5581 gaggctcgcc atctttccgt cgtagtcgta gaaagagaag gggcgggcga ggaatgggtc
     5641 tagagtagca gggtaaccgg ccgccctcgc catcacgaac tgtcccggtc gtacgggtcc
     5701 aagactccag cgatattcga gcagcgtgta ggggccgagt tcctcgcgct gttcgacctc
     5761 gacgctgtag aacttcatgc tctcgcagcg tagaggcctt gaagcgagtt cacgcgtctg
     5821 gtgccgcccc ggacccttgc ttctatgccc tgtacagcgg ccgcggcgcc tgccagggtg
     5881 gtgatacagg ggaccccatg catgagggcg cggcgccgga tcaggtaccc gtcggtgcga
     5941 ggtccgcggc cccagggagt gttgacgatc aagtccaccc caccagcctc tataagactc
     6001 aacacgtcct cccctagctc ccctatcttg ggcacgatca tgaccggtag cccgttgttc
     6061 ttgagcacct cggccgtccc ttcgctcgct gagatctcga atcccaggtc ggcgaaagcc
     6121 ctggctatga gcacgacggc cctcttctcc ctgttggcga cggagatgta gaccctcccc
     6181 gagaggggaa gctcctgccc ggccgccgtc agggccttcg cgaaggcgcc gccgaaggtg
     6241 cggtctatcc ccatggcctc gccggttgag cgcatctcgg gtccgagcag cgggtcgacg
     6301 tccgcgaacc tgtcgaacgg gaagacaggc gccttgacgg agaagtgccc gttggtggaa
     6361 gcacgtaggg ccatatccct gagcttctct ccgagaagaa ccctggtcgc tattttggcc
     6421 agcggcacgc ccgtcgcctt ggagatgaaa ggtaccgtac gcgaggcacg ggggttgcac
     6481 tcgatcacga gcacgtcgtc gcccctgatc acgaactgca cgttcatgag cccgactact
     6541 ccaaccgaga gggccaacca cctggtgtaa tcttctatcc tctgcagcag accccggtgg
     6601 atattgatgg ggggcgtcac gcacgaggag tcgcctgaat gcacaccggc ctcctcgacg
     6661 tgctccatga ttccgccgac gtagacctcc tcgccgtccg agacggcatc cacgtcgacc
     6721 tcggtgtagt cctccataaa cttgtctatg agggtagggt gtcctgggcc ggcgcccgcg
     6781 ctggtcctga ggtacaggtc gaggtcttcg tcgttgtaga cgatctccat cctgcgcccg
     6841 ccgagcacgt aagagggcct cacgacgacc ggatacccga gctcgcgcgc cacctccctg
     6901 gcctcctcgg ccgtcgatgc gctcccgaac ctggggtgcg ggatgccgag atctgccagg
     6961 agccggccga acctggagcg gtcttccgcg aggtcgatgg cgtcgggcga ggttccgagg
     7021 atccgcgccc ctgcctgttc cagctcgtgc gcgagcttca gggggctctg gccgccgaac
     7081 tgcaggatga cgccttctgg ctcctcgcgc tttatgacct cgagcacgtg ttctgcggtg
     7141 agcggctcga agtacagacg cgttgaggtg tcgtagtcgg tggagacggt ctccgggttg
     7201 gagttgacca tgaccgcgtc gtagccgctc tgtgcgaggg cgtagctggc gtggacgcag
     7261 gcgtagtcga actcgatgcc ctggccgatg cggttgggac cgctgccgag cacgacgacg
     7321 gaggcattct cctcgcgcct gacctcgtcc tcctcctcgt aggtcgagta gtagtacggc
     7381 gttcgcgcgg ggaactcacc ggcgcaggta tccacggact tgtaggtcgg cgagacgccg
     7441 agggcccgcc taacgccccg caccacctcc accgaacttc cggacgccgt cgcgatcgtc
     7501 tcgtcggtaa gcccgatcct cttcagctcg cgaaggtcct cgacgccgaa aggctcggat
     7561 acggcgcctt ccgcggccac gattctggct atagaagcga taaagaacgg gtctatggaa
     7621 gtacgttcga atatctccat aatgtccatc ccggcgcgca gggcgtcgaa gacggcgaag
     7681 acgcggtacg ggctcggctc gtcgaggccg gcatggacgt cctgggcgtc cacctccagc
     7741 gaggccatgg ccttcatcag gctctccgta aacgtcctcc ctatcgccat tacctcaccc
     7801 accgagtgca tcctggtcgt gagcctcggc gccgcaccag gaaacttctc gaacgcgaag
     7861 cggggtacct tggtgaccac gtagtccagc gcgggctcga aggaggcggg cgtcgcccgc
     7921 gtgatgtcgt tcggaatctc gtcgagggag taccctaccg ccaactttgc agctatcttc
     7981 gcgatcggga accccgtagc cttgctcgcc aaagcgctcg atcggctcac ccgcgggttc
     8041 atctcgatca cgtagtacgc ctcactctcc gggtccaccg caaactggat gttggacccc
     8101 ccggtggaaa ccccgatctc acgtattatg cgtaaagatg cggaacggag ggtctggtac
     8161 tggcggtcgg agagggtttg ggctggggcg acggttatgg agtcgccggt gtggacgccc
     8221 atggggtcca cgttttcgat ggagcagatc acgacgacgt tatccgcgag gtcgcgcatg
     8281 acctcgagct cgaactcctt ccaacccgcg acgctctttt cgacgagggc gctccccacg
     8341 gggctggctt cgaggccgtc gatgacggcg cggcggagct cgcggtggtc ggtgacggtg
     8401 gcgcctcctt tgccgccgag ggtgaagctg gggcggataa tgagtgggaa gccgatctgt
     8461 gccgccagtt cctcggcttc ggcgatgctg cggaccgtac ggctgggggg aacgttcagg
     8521 ccgattcggc gcatggcctg ggagaagagc ttgcggtcct cggctttacg gatcgagggg
     8581 atggacgctc ccaggagctc gacgcccgtc tcatcgagga cgcctgattc gttgagttcg
     8641 acggcgaggt tgagggcggt ctggcctccc agggtgggga gcagcgcatc gggacgctcg
     8701 cggcggagta tctcggcgac ggtctcggcg gtgaggggct cgacgtaggt tacgtccgcg
     8761 acctccgggt cagtcataat ggtcgccggg ttggagttta cgaggatcac gcggtaaccc
     8821 tcctcgcgca aggcgtggca ggcctgggtg cccgagtagt cgaactctgc ggcctgcccg
     8881 atcacgatgg ggcccgagcc gatgatcagg acgctctcga tgtcgttgcg ccgcggcacg
     8941 ctaggcccgc gccttctctg aaatgctgtt cacgaactgg tcgaagaggt agccagagtc
     9001 ccgcggacca gggctcgatt cgggatggta ctgcacgctc caggccctct ggtgggggct
     9061 actgatgcct tcgacggtgc cgtcgtagag gttgcggtgg gtcaactcga cgccgcctgc
     9121 gagtgaatcc tcgcttacgg cgaagccgtg gttctggctc gttatctcta tgcgtcccgt
     9181 atcgaggttc ctgacgggat ggttcgcgcc gtggtggccg aagggcagct tgtaggtctc
     9241 gcaacccagg gcgagcccga ggagttggtg tccgaggcag atgccgaacg tgggtacctc
     9301 gccgagcagc gggcgcaggg tgtcgaccgc cgctccgagg gcggccgggt cgccgggccc
     9361 gttggagagg aagaggccgt cgggctcggt ggcaaggatc tcttcagaag tggcggagcc
     9421 gggcatcgcg aggacagagg tgccgcggct acggagttcc ctgtagatcg aacctttgac
     9481 gccataatct agtgcgacga cgcagcagcg ttcctctccg aaggcgggaa ggagcgtggg
     9541 ttcggtgagc tccgagctcg tcgaggcgag gtcgaggccg accatcgaag gatgggcgat
     9601 tgcccgctcg cgcaggacgt tccggtccga ttccgaggtg gagatcacac cgcgcatcgc
     9661 gcccctgtcc ctgaggtgcc gcgtcagggc gcgggtatct attccctcca cgccgaggac
     9721 accgcactcc tcgagcaggg ctgccaggga acgctcgctc gcccagttgc tcgggtgcgg
     9781 cgtgtactcg cggacgagga cggcggccgc ctgaaccctg cgtgattcgt cgtcgccggg
     9841 gatcacgccg tagttcccga tgagcgggta ggtgaagagc aggatctggc ccctgtagga
     9901 agggtccgtg atggtctcct ggtagccgac catgctcgtc gtgaagacca cctcacccgc
     9961 gacctcgccg tggccggcga agctccaacc ccggaaggcc gccccatcct cgaggaccag
    10021 cgtcgccctg ctccgccctc cgttacgcac gtgctccaaa ctttgctccc gttctgtcgt
    10081 gtaccggctc tccaccaact atagtaccca cgaccctgcc cttcagcgtc tttccgagat
    10141 agggggaatt ggacgagcgg cttacgagcg tagcactccc taccgtccac tcctccgcaa
    10201 ggtcgacgag cgtgagatcc gccggcgcgc cgacggccag ggtgccccct gtaccgaccc
    10261 agcggccagg gccgctgccc atcgcttcga cgaggcgttt cagcgagagc gcgccgccga
    10321 ggaccagttc ggtgtagagg gcggcgaagg ccgtctcgtg gccgaggaac cctggcgcgg
    10381 cctcttcaat cggcagttct ttctcttccg gggcgtgggg cgcgtggtct gtagcgacga
    10441 agtcgaggac ccggtcgcga agggccggcc cgaggccggc gcggtcgtgc tcgggcctga
    10501 gcggcgggtt gacgcggtag agtccgtcga gcgtcgagac gagatcttct gtcagggtca
    10561 ggtggtgggg cgtcgtgtcg gcggtaattt cggccctgtc cctgaagaag ccgacgagcg
    10621 cggccgagag ggccgtggag acgtgggtta tatgcaccct ggcccccgtc tcggcggcga
    10681 ggatgagggc cgtcgcggtg gcggcgtctt ccgcgctggc cggggagccc gggatgccgg
    10741 cgagcgaggc ggccaccccg tcgtgcacta cgccggtagc cagcgtgtgg tcttcgcagt
    10801 gcaggatcac gggcagcccc gcagagcggg cgtagagcat accgttcctg aggacgcccg
    10861 ccgaagccgt ccccagcccg tcgtcggaga cgcagaacgc gccggcttgc ttgaggaggc
    10921 gcatctccgt cagcctctcg ccggcgagcc cgacgtggag ggccgccgca acgcgggccc
    10981 gcaccctcga ctcccgctcc acacggcgga cgagaccgct gacaacgacg ggacggtcca
    11041 cgatggggtc cgtgttcggc atcatgacca cgcaggtgaa gcctccggcc gcagcagcgg
    11101 ccgagccgct ctcgatatcc tcctcgtcct cgcgccccgg cgtacgccag tgggcgtgga
    11161 cgtcgacgaa gccagggaac agatgcaggc catcggcgtc caaagtgcgc ggcccgcgaa
    11221 ggtcctcgcc gatctccgcg acgcggcccg atgagaggcg tacgtccagg atcccgtcca
    11281 ggcccgtagc agggtcgaga acgtgggcat gtcgtatgac caactccgca ggatagccgc
    11341 ctctcagcat tacgcggcta cgctctggac ggcgcccgtc gagagggcga ggacggccga
    11401 acggacacag acaccggcgg cgacctggtc ggggatcaag gacgaggcgt cgaggacgac
    11461 gtcgcccgag atctccaccc ccctgttgac cgggccagga tgcatgatca gcgcaccacc
    11521 ctccaggtgg cgccgcgaca caccatagaa cctggcgtac tccgcgacgg agggtatccg
    11581 cgcgcccgtc atcctctccc tctggagacg cagcatgtag agaacgtcag cgccccactc
    11641 gagcgcctcg tccacggaag cgagtacggg cagatcaaag acaccctcat ccggcggcag
    11701 gagcgtcctg ggtgccacga gagcgacctc catcccagcc tgcaagaagc cgggtatgac
    11761 gctgcgcgcc accctgctgt gcagaacgtc gcccacgacg gcgacgcgtc gatcctggag
    11821 tccgtcgaag ccgccgagcg cctcgccgag ggcgtagagg tcgaggaggg cctgagtcgg
    11881 gtgctggccg cagccgtcgc cggcgttgat caccggagcc gaggtgtggc gggcggcgag
    11941 ggctgccgct cccgcggccc ggtgccgaag cacgatcgca tccgccccga ggcggtcgag
    12001 ggttaccacg gtgtcgatca gggactcgcc cttggcgatg gcagagccct tctcggagag
    12061 cgagatcaca tgcgccccag aacgcctcgc ggcgagctcg aaggagacgg cggtcctcgt
    12121 cgacgcctcg aagaaggcca ggcaaacggt cttgccctcg agcgctcccc tgaggcgtcc
    12181 ctccgcaaac gccctggcca ggtctagtat ctccctgata tcctcgcgtc cgatgccttc
    12241 cagggtaagc aagctcctag tctccaacga tgatcacccc gtcctcccca tcggtctcct
    12301 ccaggttgac aagcactctt tcgtcgcgcg cggtgggcac gttcttaccg acgtagtcgg
    12361 cccgtatcgg taactctctg tggccgcggt cgacgagaat cgccaacctg accgcggccg
    12421 gacgcccgag ctcgaggagc gcatccatcg cggcgcgcgc ggtcctcccc gtgtagagaa
    12481 cgtcgtcgac gaggatgacg gtcttgcccg tcacgtcgaa cggcacgttg ctgccccgca
    12541 cttcgggatc cttcttttcg aggtcgtcgc gatggagcgt tatatccaga gagccgacgg
    12601 ggagctcgag gccttcgaac tcgcggacgt tcgccgagat cctctgcgct agagggactc
    12661 cccgggttag gatcccgacg agcgcgagcc tgtcgaggca agaggcgttt cgttccagta
    12721 tctcgtgaga gatgcggcgg agggaacggt taagtccgtc gggggcgagc accttggaat
    12781 gcgcgtagac gctcgttcgg cccaagcctg cctccttcgc gtctcacggg acgccgttaa
    12841 aggatcgcca tgccgaaggt caagctacca gagggcgagg gagccgtcaa gcccgaccgc
    12901 cgagtcccgc aagtcctcgg ggatctcgga gcgaaacacg agggcctcgc cacttacggg
    12961 atgttcgaag gagagccgtt ctgcgtgtag ccagagacga tggccgggga ccggatcgcc
    13021 gtacaaaggg tcggcgtaga tcgggtatcc gatggccgag aggtggaccc tgatctggtg
    13081 cgtcctcccc gtctcgagcc gtacgcgcag catcgtatgt tctgccgctg tggcaagcac
    13141 ctcgaagtgt gtaacggccg gcttaccaac gcctgcggcc atgagggtgg ggttgtccgg
    13201 atcgcggccg acgggggagt cgatcgtccc ggtccggggt aggccttcgc cccccacgag
    13261 ggccctgtag aggcggccca ctcgcctggc agacaacatc ccgacgagcc tggagtacgc
    13321 cggctcgcct ttcgccaaaa ccataaggcc cgatgtgtcg cgg
//
LOCUS       DATMVF010000024        15326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_231845, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000024 DATMVF010000000
VERSION     DATMVF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15326
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231845"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VN178_00930"
     CDS             <1..335
                     /locus_tag="VN178_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79555.1"
                     /translation="ARAMAVDADILLMDEPFASVDEQIRRKFQEDLLELLSDEHKTVV
                     FVTHSIEEAVYVSDQVVIMTRGPGRASKLVRPDIDREGGSDKIRRSPEYLDWVERIWV
                     ELRDYLEQ"
     gene            352..1098
                     /locus_tag="VN178_00935"
     CDS             352..1098
                     /locus_tag="VN178_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWS79556.1"
                     /translation="MGGYKIPIAFSLLVWCLVWEVVGRLGLISLIPPFTEVLGAMPEV
                     MGSASFVEAAAITFQAYGIGMALSLTIGIGLGILMGRVRAIGELMGMWVNIFESSPLT
                     AVVPALIALLGFGLSTMIVTVFVFSVWVIALDAQVGVERVNPSLVEMSRSFGASKRDL
                     YVKILFFAALPELLAGIRLGLIRGLKGVIIGQLLIAVVGMGYLFELYSRSFLMAEFWA
                     LLIVVFASAFLASEAVAYFERKVSYYATAR"
     gene            1114..1803
                     /locus_tag="VN178_00940"
     CDS             1114..1803
                     /locus_tag="VN178_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWS79557.1"
                     /translation="MTYAVQPPPVTTLPVSGTEETFPVSRVYCVGRNYAEHAVEMGHD
                     PDRELPFFFAKPANAVVPNGGTLAFPSSTEDLHHEIELVVALGEGGADLTASAALSHV
                     FGYAVGLDMTRRDLQAEAKKAGRPWDMAKGFDRSAPMSAICPVSAIGHPERGSIWLRV
                     NDELRQQGDLRQQIWKVPETIAFLSTLVELRPGDLIMTGTPKGVGPVHRGDQLVGHID
                     GVGDLKVGYRA"
     gene            complement(1865..3037)
                     /locus_tag="VN178_00945"
     CDS             complement(1865..3037)
                     /locus_tag="VN178_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79558.1"
                     /translation="MRAPKESSGGQRLLQLAAFFSSFDRFAIAPMLLTIAASFGASLT
                     EVAATASLYYLLYGLMQPVWGMLSDRLGRVSVMRLTLFGAAFAGILSSLAPNLSVLIV
                     ARALAGGLFAAVIPASLVYIGDTVGMGSRQKALADLMAASAVGTALATVFGGLAAYLD
                     GWRVAFAGPALAGAALAVLLARLPEPMVLSDQKREGPVVQAGRVLVQPWAITVVGIAL
                     VEGAVILGFLTFFAPSLESVGFSPAVAGLAVGLYGLAVLGWTRIVKLIANRLGASALI
                     LIGAGMLSLGYASGAVGQSLASVSAAAILVGGGFAFMHSTLQTWATEVVPEARATVIS
                     LFAAALFAGSGVAAMAAAPLAEAGSFDLLFALGALVAVPLGLFAGLARHRYSRIRR"
     gene            3350..4165
                     /locus_tag="VN178_00950"
     CDS             3350..4165
                     /locus_tag="VN178_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79559.1"
                     /translation="MVRRNIGTVLVAAAVTVLLLVAYVLARAQHKLPELGLDLLVYLF
                     ATTLAVFVIGRILDWREKRRWEVAEDWLYVILLETIDDLLKVLLPAAVPREGADEEVT
                     VYEVAGERIHFGETVAYSPLRLFVSPGEKDLQSHILWYASELGPPRYAESARAALSDA
                     RAQVRDMLATSGQLLEADITAMVMSFDQAVAAAIRHVDSAASMRNEKAEDSTFQDGGL
                     STTQRTSEADQELTFVSSIIVESVVDSAIKPKAWLETELVNREGRSPFRRLRT"
     gene            complement(4345..5649)
                     /locus_tag="VN178_00955"
     CDS             complement(4345..5649)
                     /locus_tag="VN178_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79560.1"
                     /translation="MSVAATGERTQPGGVRKRAVVAAVIGNFIEWYDFTLYGYLAVVI
                     APLFFPAENRLVSLMATFAVFGVAFVMRPIGALVFGSIGDRIGRRNTLATIILMTSGA
                     TFFMGLVPSYETIGILAPILLVLARVVQGFAVGGEYGGATAFMVEYAPDDKRGLYGSW
                     QFVTQGASLMVGLLIVTVLTTVLSEEALFSWAWRLPFLLALPLGLIGLYMRLRLEDTP
                     AFEAVREHHEVERTPLKETLRHHGKDVLKVVGLTWLLTGLTYLLVFMPTYLSEELGVP
                     LSSAYTAVILGTAGYCVTVLVAALLSDRIGRRKPFLLTAPVVAVFAAVPIFLLIQTAN
                     FAAITLALVIAGVILGSSAGVYAAATCEVFPTNVRYSSLSLGYSVAVSIFGGTTPLIF
                     TSLLAATGNPISPAFYLIAAALAGIVAALTFPETAREPLHET"
     gene            complement(5747..6862)
                     /locus_tag="VN178_00960"
     CDS             complement(5747..6862)
                     /locus_tag="VN178_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79561.1"
                     /translation="MERRKNGPGKEDPESAANASKSSPNPGQTAESSSPETSEDHADP
                     EVMTPEQEARRESEERIKGLLEAYQQTLDTVEELPAVVWVPWDRKGPGRYFKVPYLRL
                     FLIDFVAYHIDRSLNTLNRRFLATAALSGDPDANWSTREAVKLYLQSLPRPPYTILIF
                     LALLAALVIALPLQGFGNVFYVLDLVGAILRFNVGYVGRALEGNELGPTIRSLIVLLV
                     GLIGVGALLTSPFGLKRILFNLHPWTRERFDSTAARSHGSRVEGLYALEESIFSEAGI
                     QPPKEGKWDLVFQAFLLLLMLVIGLCLAVLTLFYAMSWDLRLNVETGAPTDVHFTLPN
                     VHWGYFALFTGLVFAAAVLLLKRLVAAWKKRGRNAVG"
     gene            7259..8080
                     /locus_tag="VN178_00965"
     CDS             7259..8080
                     /locus_tag="VN178_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79562.1"
                     /translation="MAKSMALWSSFVEVDPFTWSPSKARETFETGIREAAERGLVPVL
                     SAERLSGNPDSGGYDSVHVAEYLAATFPEARVLIVIREQADMLVSSYKRYVRNGGPGT
                     LRQYGSPPWGPPRPPHFDLSYFEYHRLIGLYHDLFGPENVLVLPYELLSDRPRTFLKR
                     LGGFAGAATAADSHFEPVNASLSALSLAFKRHANRWVVRDPLNPAPPFEIDGANQRLL
                     ELCAAADPKIPSSLRRRSERRLRNISRDIVGDRYAESNAATSRLTNLDLHSYGYA"
     gene            complement(8043..8966)
                     /locus_tag="VN178_00970"
     CDS             complement(8043..8966)
                     /locus_tag="VN178_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79563.1"
                     /translation="MPREVRENFPPDMLALRRFVVWRYEERVGKPAKIPHVADGTGAR
                     ASSTDPSTWRGFEAAARVLLAGGYDGVGFVFSPGDPYAGVDLDDCRDPESAALEAWAR
                     ELVATLEGYAEVSPSGTGVHVIVRGAAPNRQDGNVEAYSSGRYFTVTGRAISGHGLPP
                     PERQEGLNMLAQKYLAGATGTSEIARSRRGEPGRLGDMEVDDLCRSACGGEEYEYLVG
                     SGGIAGSGDGPLGAERALARLIASHTQDAAQVGRLMDRSLLGQRPEWRGHVDHRTRTI
                     EFAIAEVLGERCNQPTEPVVRRIRTSADRGS"
     gene            9266..10126
                     /locus_tag="VN178_00975"
     CDS             9266..10126
                     /locus_tag="VN178_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HWS79564.1"
                     /translation="MSADTHLVIATMGGTTVREKDLIDRTQVPRTRQSLAEDLRRLGL
                     KTGAVALVHSSLSSLGWVCGASVAVVQALMDVITPSGTLVMPTHSGEYSDPANWEHPP
                     VPSSWQGPIRESMPAFDPQTAPFRGMGRVPETFRTWPGALRSSHPAVSFAAWGRHAEF
                     VTQEHSLDHSLGEGSPLARIYELDGRVLLLGVGHESNTSFHLAEYRAPGARRIEAGAP
                     ILEGGRRVWKTYEDIELDEEVFEEIGADFENAVGLRYFRVGSARARLFSQREAVDFAR
                     GQIAHRRRYS"
     gene            10334..10897
                     /locus_tag="VN178_00980"
     CDS             10334..10897
                     /locus_tag="VN178_00980"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWS79565.1"
                     /translation="MFTRIESERLILRRFEDSDLIPFVAYRNDPEVARYQSWDSCDEQ
                     EAMVLIREMGAARVGVPGEWFGFAIESKETGSLIGDCALRVDEHEPYRAEVGFTLARE
                     YQGRGLASEAVARVLDYAFGALGLHRIIAIADCRNASSVALLERLGMRREGHFVENTW
                     FKGRWADEYSYAVLRHEWLSKRSGVSP"
     gene            complement(11211..12131)
                     /locus_tag="VN178_00985"
     CDS             complement(11211..12131)
                     /locus_tag="VN178_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79566.1"
                     /translation="MRIYAGNGPPEPTISSPAQDKLFAVGEKIVLRGSATDPQDGELP
                     DSALGWEVVQHHNGDHTHPHFAGTGNDLTFTAADAEDLLSTGPEENYLEIRLTATDSE
                     GLSKTVTQKLPPKTVDVTFESRPQGLKLRLNGTTFTTPRTFVSWEGYKLRVTALSPQA
                     LAGSTHTFDAWSDGGTQDHEIFTGAQPGTYTATFKACTITGTSGNDVLEGTLGADTIC
                     GMAGNDTIKGMAGNDTIDGMGGDDPVLRGDGGGDKVTGGGGTDNLNGGSANDALISRD
                     GVSGNDSLDGGTGTDTRTTDASEKSIVGFP"
     gene            complement(12141..13376)
                     /locus_tag="VN178_00990"
                     /pseudo
     CDS             complement(12141..13376)
                     /locus_tag="VN178_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
     gene            complement(13395..13685)
                     /locus_tag="VN178_00995"
     CDS             complement(13395..13685)
                     /locus_tag="VN178_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79567.1"
                     /translation="MRREIHLRMVRHVVLLAVVALSAVVVLVFLHANGGVTATLRSAE
                     FDDRSVTPIFSPIDLAFAPDGRMLIATQPGLLRVHKDGELLQTPALDLTGKV"
     gene            14296..14691
                     /locus_tag="VN178_01000"
     CDS             14296..14691
                     /locus_tag="VN178_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS79568.1"
                     /translation="MGGEDMTGERARDPGDLLPLTPVVLNVLLALADGERHGYGIMLE
                     VRERTGGRVRLGPGTLYGAIKRLKEGGVIEESGQRPDPAEAPDERRRYYRLTGFGGEV
                     LAAEIERLDGLVRAARRKGAFPAPNPEGA"
     gene            14691..>15326
                     /locus_tag="VN178_01005"
     CDS             14691..>15326
                     /locus_tag="VN178_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79569.1"
                     /translation="MADPGGPSRGGHERAVGTSEWVYRSLLRAYPRGLREEYGEEMAR
                     CFRDLCRERLEVGGGLGLAALWAHILTELLCTALKERSTTLDRNTYRSVVGVALATAF
                     ILLLPLLAAPAWSLADFVVAGALIFGTGLTYVLVARKAGKIAYRAAVGVALAAALLLV
                     WINLAVGVIGSEDDPANLMYVGVLFVGIVGAIVSRLRPDGMARALLATALAQ"
BASE COUNT         2807 a         4812 c         4884 g         2823 t
ORIGIN      
        1 tggcccgagc catggcggtt gacgctgaca tcttgctgat ggatgaaccc ttcgcttcgg
       61 tggacgagca gatccgtcgc aagttccaag aagatctcct cgaactgctc agcgacgaac
      121 acaagaccgt cgtcttcgtg acccactcga tcgaggaagc ggtctacgtc tcggaccagg
      181 tggtgatcat gacacgcggc cccggacggg cctccaagct ggtgcgccca gacatcgacc
      241 gggaaggcgg ttccgataag atccggcgca gccccgagta tctcgactgg gtcgagcgga
      301 tctgggtcga gctacgggac tacctcgagc agtgatggag gagagatgac gttgggcggg
      361 tacaagatcc cgatagcctt ctccctgctg gtctggtgcc tggtctggga ggtggtgggc
      421 cggctcggtc tgatctccct gatcccaccc ttcaccgagg tcctcggggc gatgcccgag
      481 gtgatgggct cggcgagctt cgtcgaagcc gccgcgatca cgttccaggc ctacggcatc
      541 ggcatggcgc tgtccctgac gatcggcatc ggcctgggca tcctgatggg cagggtccgt
      601 gcgataggag agctgatggg gatgtgggtg aacatcttcg agagctctcc gctgacggcc
      661 gtggtgcccg cgctcatcgc gttgctcggt ttcggcctct ccacgatgat cgtcaccgtg
      721 ttcgtgttct cggtgtgggt gatcgctctg gacgcgcagg tgggcgtcga acgggtgaac
      781 ccctccctgg tggagatgag ccggtcgttc ggtgcctcca agcgcgacct ctatgtgaag
      841 atcctgtttt tcgccgcact ccccgagttg ctggcgggga tccgcctcgg cctgatccgc
      901 ggcctgaagg gcgtgatcat cggccagctg ctgatcgcgg tggtggggat ggggtacctc
      961 ttcgaactgt actcgcgaag cttcctgatg gccgagttct gggcgctgct gatagtggtg
     1021 ttcgcatctg ccttcctcgc ctcggaggcg gtcgcgtact tcgagcgcaa ggtgtcttac
     1081 tatgcaaccg cccgttgaga ggacgtgcat cctatgacct acgccgtgca gccgccgccc
     1141 gtaaccaccc tgcccgtgtc cgggacggag gagaccttcc ccgtctcccg ggtgtactgc
     1201 gtggggcgca actacgccga acacgccgta gagatgggcc acgacccgga ccgggaacta
     1261 cctttcttct tcgcgaagcc ggccaacgct gtggtaccga acggcggaac cctggcgttc
     1321 ccgtcatcaa cggaggattt gcaccacgag atcgagctcg tggtggcgct gggggagggc
     1381 ggggctgacc tcaccgcatc ggccgccctg tcccacgtgt tcgggtacgc cgtgggtctc
     1441 gacatgaccc gccgcgacct gcaggctgag gcgaagaagg ccggacggcc ctgggacatg
     1501 gccaagggct tcgatcggtc cgccccgatg agcgccattt gtccggtatc ggcgatcggc
     1561 cacccggaac ggggatcgat ctggctgcgc gtcaacgatg agctccggca gcagggggac
     1621 ctccgccagc agatctggaa agttccggag accatcgcgt tcctgagcac cctggtggag
     1681 ctgcgacctg gagacctgat catgaccggg acacctaagg gggtcgggcc ggtgcaccga
     1741 ggtgaccagc tggtgggaca catcgacgga gtcggcgacc taaaggtcgg ctaccgggcg
     1801 taacgcgcta ggcgacgtcg agacttgtcg agaccgggtc gcagacgact gccagcgtgg
     1861 agatctagcg ccgaatacgg gaatatctgt ggcgtgccag gcccgcgaag agtcccagcg
     1921 gaacggccac aagggcgcct agggcgaaga gcagatcgaa ggaccccgct tccgcgagtg
     1981 gggccgcggc catggcggct accccgcttc ccgcaaatag tgcagcggcg aacagcgaga
     2041 tcacagtagc tcgcgcttcc ggcacgacct cggtggccca ggtctgcaga gtggagtgca
     2101 tgaaggcgaa ccccccgccg acgagtatcg ccgcggcact cacgctggcg agcgactgtc
     2161 ccacggcacc cgaggcgtaa cccagcgaca gcatgccggc gccgataagg atcagcgcgc
     2221 tcgccccaag gcgattagcg atgagcttga cgatccgggt ccagccgagc acggcaagcc
     2281 cgtagagtcc gactgcgagc ccggccaccg cggggctgaa gccgacactc tccagagaag
     2341 gagcgaagaa ggtgaggaag cccagtatca cagccccttc gaccagcgca atccctacca
     2401 ccgttatggc ccacggctga acgaggacac ggccggcctg gacaactggc ccctctcgct
     2461 tctggtcgga gaggaccatc ggctccggca atcgggcaag caagacggca agcgcagcac
     2521 cagcgagggc aggacctgcg aaggcgaccc tccagccgtc gaggtacgca gcaagccccc
     2581 cgaatactgt tgccagggcc gtaccaacgg ccgaggcggc catcagatcg gcgagcgcct
     2641 tctgccgaga tcccataccg accgtgtcgc cgatatagac cagggaggcc ggtatcaccg
     2701 ccgcgaagag ccccccggcg agcgctcgtg cgacgatcag cacactcaag ttcggggcca
     2761 acgacgagag gatccccgca aacgcggccc cgaatagcgt caggcgcatc acgctcaccc
     2821 gccccagcct gtccgagagc atcccccata cgggctgcat caagccgtag agcaggtagt
     2881 agaggctggc cgtcgccgca acttcggtaa gcgacgctcc gaaggaggcg gcaatggtca
     2941 agagcatcgg cgcaatggcg aagcggtcga agctgctgaa gaacgccgcc agttgcagga
     3001 gccgctgccc tcctgaagac tctttcggcg cacgcactat cgcggtccct gtttcactga
     3061 tccgacagcc tccatttatg tcccgcgtgg acgtagtgcc tcacgggcaa gctcctgcct
     3121 cattctcgtc ccgggccata cttccgcatc ttacccgcac accgagacca acaagcacag
     3181 atgagcgatc tcaatccagc tttcggtacc gaatcaccca tcctgtgatc cctatcgtgc
     3241 tgattgaagg ggcaaagttg tagcggtttc ctaacgatgg ctctagaatc cttcgcaggc
     3301 aactttgtgg gtggcagggg ataaagctag gagaaaggga gaaggaataa tggtcagacg
     3361 caacatcggg acggtcctgg tcgcggctgc cgtaaccgtc ctcttgctcg ttgcgtacgt
     3421 tctcgcccgg gcacaacata agttacccga actgggcctc gaccttctcg tgtatctctt
     3481 tgcgaccacg cttgccgtgt tcgtcatcgg tcgaatactc gactggcgag aaaaacgccg
     3541 gtgggaagtg gctgaggatt ggctgtacgt gatcctgctc gagacgatcg acgatctcct
     3601 gaaagttctg cttcctgccg cggttcccag ggaaggggcc gacgaggaag taaccgtcta
     3661 cgaagtagcc ggcgagcgga tccatttcgg cgagaccgtc gcctacagtc cacttcggct
     3721 gttcgtgagc cctggggaga aggacctgca gtcccatatt ctctggtacg ccagcgaact
     3781 gggaccgcca cggtacgcgg agtcggccag ggcggctttg tctgacgcca gggcgcaggt
     3841 acgggatatg ttggccacct ctggccagtt actagaggcc gacattacgg ccatggtgat
     3901 gagtttcgac caggcggtcg cggccgccat aagacacgtg gactcggccg caagcatgcg
     3961 aaacgaaaag gcggaagact cgacgtttca ggacggaggc ctatcgacaa cgcagcgtac
     4021 aagtgaagcg gatcaggaac tgaccttcgt tagcagcatc atcgtggagt ccgtggtcga
     4081 ttcggcgatc aagccgaagg cttggctcga aaccgagttg gtcaaccggg aaggtagatc
     4141 tcctttccgg cgcctccgaa cgtagatgcc gttacccgcg aatgctctcc agccctcctc
     4201 ctggcaaaac ggagggaaag ctaccccgct cctggcgcaa acagcggcga ggtgtgtggt
     4261 cagcccctga gtacggccct ggaagtccgt ctctgggggc gttcagtttc gccgggtcag
     4321 gttcctctgt cttggggcag gaactcacgt ctcatgcaag ggctccctgg cggtctcggg
     4381 gaacgtcagg gcggcgacga tgccggcaag agcggcggcg atgaggtaga aggcgggcga
     4441 gatcgggttg cccgtggccg ccaggaggga ggtgaagatc aggggtgtcg taccgccgaa
     4501 gatggagacc gcgacgctgt agcccaaaga gagtgagctg tagcggacgt tggtggggaa
     4561 gacctcgcac gtggcggccg cgtagacccc cgcagagctc cccaggatca cgccggctat
     4621 cacgagcgcc agcgtgatgg cggcgaagtt tgcagtctgg atcagcagga agatgggcac
     4681 ggcggcgaag acggcgacca ccggcgcggt gagcaagaac ggcttcctcc tgccgatacg
     4741 atcggagagc aaggccgcga cgagcaccgt cacgcagtac cccgccgtcc ccaggatgac
     4801 cgccgtgtag gccgaagaga gcggcacgcc gagttcctcg gagaggtagg tcggcatgaa
     4861 gacgagcagg taggtgagcc ccgtcaggag ccaggtcagg ccgacgacct tcaggacgtc
     4921 tttaccatgg tgcctcagag tctccttcaa cggtgtcctc tccacctcat ggtgctccct
     4981 gacggcctcg aaagccggcg tgtcttcgag cctcaagcgc atgtagagcc cgatcaggcc
     5041 gagtggcaac gcgaggagga acggcaggcg ccaggcccag gagaagagtg cctcctctga
     5101 gagcaccgtc gtcagaaccg tcacgatcag gagtccgacc atcaatgagg cgccctgggt
     5161 gacgaactgc cagctaccgt agagcccccg cttgtcatcg ggggcgtact cgaccatgaa
     5221 cgcggtagcc ccaccgtact cgccaccgac ggcgaacccc tgcaccacgc gggcgagcac
     5281 cagaaggatc ggcgccagga tcccgatggt ctcgtaagaa ggcaccagac ccatgaagaa
     5341 ggttgccccc gacgtcatga ggatgatggt tgcgagggta ttgcggcgac cgatcctgtc
     5401 gccgatgctg ccgaagacca gcgcgccgat ggggcgcatc acgaacgcca caccgaagac
     5461 cgcgaaggtc gccatgagcg agaccagacg gttctccgcc gggaagaaga gcggtgcgat
     5521 cacgaccgcc aggtagccgt agagcgtaaa gtcgtaccac tcgatgaagt tgccgatcac
     5581 cgccgcgaca acggccctct tgcgcacccc acccggctga gtcctctccc ctgttgctgc
     5641 tacggacatc ctcccacact ccttcctccg gtcaccggcg ttcgatgcac tttatcaccg
     5701 ccgcacactt tgccctcgaa tcaccacata cgtgcgcttg gcggcgtcaa ccgaccgcgt
     5761 tccggccgcg cttcttccag gctgcgacga gtcgtttgag gaggagaacg gcagcggcga
     5821 acacaagccc ggtaaacaga gcgaagtaac cccaatgcac gttcggcaat gtaaagtgaa
     5881 catcggtggg cgctccggtc tccacgttga gtctcaagtc ccacgacatg gcgtagaaga
     5941 gtgtcagaac cgccaggcag agtccgatta cgagcatgag caggagcaag aaggcttgga
     6001 acaccaggtc ccatttcccc tccttcggag gctggatgcc ggcttcgctg aagatgctct
     6061 cttccagggc atagagtcct tcgaccctgg agccgtggct cctggccgca gtcgagtcga
     6121 agcgctctct ggtccacgga tgcaggttga agagtatgcg cttcaagccg aacggagagg
     6181 tcagcagggc gcccaccccg atcaacccga ccagaagaac gatcagggaa cgaatcgtgg
     6241 ggccgagttc atttccttcc aatgctctgc ccacgtagcc tacgttgaat ctcaatatcg
     6301 ctccgaccag gtcgagcacg taaaatacgt ttccgaaacc ctgtagtgga agcgctatca
     6361 ccaacgccgc gagcagcgcc aagaagatca ggatcgtata aggcggccta ggcaaagact
     6421 ggaggtagag tttcacggcc tcgcgggtcg accagttggc gtcagggtcg ccggagagcg
     6481 cggctgttgc caggaaccgg cggttgagtg tgttaaggct cctgtcgatg tggtatgcga
     6541 cgaagtctat gagaaacaat cgaaggtagg gaaccttgaa gtagcgtccc ggacccttcc
     6601 gatcccatgg cacccagacc accgcgggaa gttcttcgac ggtgtccagc gtctgctggt
     6661 acgcctccag gagacccttt atacgctcct cggactcccg tctggcctcc tgctcgggtg
     6721 tcatgacctc cgggtcggcg tggtcctcgg aggtctccgg gctggatgac tccgccgtct
     6781 gacccggatt cggcgagctc ttggaggcat tggccgcaga ctctggatcc tccttacccg
     6841 ggccgttctt ccttctttcc aacgcgatct ttgcctttca ccctgtggct cctggatagg
     6901 cccaagccta gttgaaaccg atcctagttt acttaatgga ggcaccgcat gcgaggtttc
     6961 tggggttgga ggcttacccc gccacgtcct ggccgacttt cgcactgagg gcattgaaaa
     7021 tccaccgggt taatgatgac ctgcacagca gtacgttagc atgagcgagg gcgaagtcgc
     7081 agaggcgggc acatcgtcac cccgacagaa cgtcgcattg gtatcttctc aagcgcctgc
     7141 gctggacctc cgtagcttat actgcgcaac gatgcgaccc ctcttgcaca tcggttacta
     7201 caagaccgga actaccttcc ttcagacacg gatcttccct gagccgggct ttttcctggt
     7261 ggccaaatcg atggcattgt ggtcctcgtt cgtagaggtt gatcctttca cctggagccc
     7321 ttcaaaggcc cgcgagactt tcgagaccgg catacgagaa gcggccgagc gtggtcttgt
     7381 gccggtgctc tcggcagaac gcctctccgg caacccggac tccggagggt acgacagcgt
     7441 acatgtggcc gagtacctcg cggcgacctt tccggaggcg cgcgtgctga tcgtgatacg
     7501 ggagcaggca gacatgctcg tttcgagcta caagcggtac gtgaggaacg gcggtcccgg
     7561 aacgcttcgg cagtatggtt cccctccctg gggtcccccg cgccctccac acttcgacct
     7621 cagttacttc gagtaccacc gtctgatcgg cctctaccac gacctcttcg ggcccgaaaa
     7681 cgtgctggtg ctgccttacg agctgcttag cgatcgacct cgcacgttcc tgaaacggct
     7741 cggcggtttc gccggcgcgg ctacggcggc cgattcacac ttcgagccgg tgaacgcttc
     7801 cctgtccgcg ctctccctag ccttcaaacg gcacgccaac cgttgggtgg tgcgcgatcc
     7861 tctcaacccc gccccgccct tcgagataga tggcgccaac caacgcctgc tcgaactctg
     7921 tgccgccgcc gaccccaaga taccgtccag cctgcgcagg cgcagcgagc gccgactgcg
     7981 gaacatctcg cgggacattg tgggcgaccg atacgctgaa agcaacgccg cgacttcgcg
     8041 actcacgaac ctcgatctgc actcgtacgg atacgcctga cgacaggttc agtcggctgg
     8101 ttgcaacgct ccccgagcac ctcggcgatc gcgaattcga tcgtccgcgt gcgatggtcc
     8161 acgtgccccc tccactccgg ccgttgcccc agcaacgaac ggtccatcag gcgcccgacc
     8221 tgagcggcgt cttgcgtgtg cgaagcgatg agtcgcgcca gcgccctctc cgcccccaaa
     8281 ggaccgtcgc cgcttccagc tatgccgccg ctaccgacga gatactcata ttcttcacca
     8341 ccgcaagcgc tgcggcataa gtcatccacc tccatgtcgc caagcctacc gggctcgccc
     8401 ctccgcgagc gggcgatttc gcttgtcccg gtcgcacccg cgagatactt ctgggccagc
     8461 atgttcaggc cctcctgccg ctcgggcggc ggcaacccat gaccggagat cgccctcccc
     8521 gtcacggtga agtagcgccc ggagctgtag gcctcgacat tgccgtcctg cctgttcggg
     8581 gccgcacccc gcactatgac gtgcacccca gttccgctcg ggctgacttc cgcgtaccct
     8641 tccaaggtgg cgaccagctc acgggcccac gcttccagcg cggcactttc cgggtcgcga
     8701 caatcatcga ggtccacccc cgcgtagggg tcgccggggg agaacacgaa gcccactcca
     8761 tcatacccgc cggcgagcaa cacgcgcgcc gccgcctcaa agccgcgcca ggtcgagggg
     8821 tcggtactgg aggccctggc gcccgtgccg tccgccacgt gcgggatctt ggccggcttg
     8881 ccgacgcgct cctcgtacct ccaaacaacg aaccggcgca gcgccagcat gtcaggaggg
     8941 aaattctcgc gaacttctct gggcaaagcc tccgcgcgtg ccgagctccg gttgcttggc
     9001 atacctctac tccgcatcat ccccgtgcca ccgtggatcg ccctcgaaga ccctctcgca
     9061 gaggtcggcc aggagcgcgt cccgctgcgg atccggccgc tggtccctcc tccataccct
     9121 agcgctcccc accctcatcg gatgcattgt cccaaaaacc tcaccgcgcg tacaaatcgc
     9181 gactagactt tgggacactt cagccaagga atggacacgc cattgcccct aaaatgggtc
     9241 ttgccaatgc ccgccggata cgctgatgtc ggcagatacg cacctggtta tagcaacgat
     9301 gggagggact acggttagag aaaaggatct catagaccgg acacaggtgc cccgcacccg
     9361 ccagagcctg gcagaggatc tgcgaaggct cggactcaaa acgggggcgg tcgcactagt
     9421 tcactcttca ctgagctccc tcggatgggt ctgcggtgct tccgtagcgg tggtgcaggc
     9481 attaatggac gtgatcacac cgtcgggaac gctcgtgatg cccacccact ccggggagta
     9541 ctcggatccc gcgaactggg agcaccctcc cgtcccgagt agctggcaag gacccatccg
     9601 cgaaagcatg cccgccttcg atccgcaaac ggcgccgttc cgcgggatgg gccgcgtccc
     9661 cgagaccttc cgcacctggc ccggcgcgct tagaagctcg catccggccg tctcgttcgc
     9721 cgcgtggggc aggcacgcgg aattcgtcac ccaggagcat tcgttagatc actccctcgg
     9781 tgaaggctcg ccgctggccc ggatctacga gttagacggg cgggtgctct tgctgggggt
     9841 cggccacgag agcaatacct cgtttcatct ggccgagtac agggctcccg gagcgcggcg
     9901 gatcgaggca ggggcgccga tcctggaggg cgggcggcgg gtatggaaga cctacgaaga
     9961 catcgagctc gacgaggagg tgttcgagga gataggcgcg gacttcgaga atgccgtcgg
    10021 gttgcgctac ttcagggttg gatcggcgcg agcaagactc ttttcccaga gggaggccgt
    10081 ggacttcgcc cgggggcaga tcgcccacag acggcggtac tcatgacgtc gcctctgtcc
    10141 acgattatga tgtcggcatc ccaagatccc gacccgggcg caactctgca cagtatcgga
    10201 caaacgaagg gacagaggac agctcattca tgcagggttc gcacacgcct gcagagctca
    10261 gccactacca accgcccact cataatgatc ggaaatgcgg gccgagtccg ctcgtcggca
    10321 gatgagtgag gttatgttca cccggataga gtcggagcga ctcatcctca ggcgcttcga
    10381 agactccgac ctgattcctt tcgtggctta ccgcaacgat cccgaggtgg cccgttacca
    10441 aagctgggac tcctgcgacg agcaagaagc gatggtcctc atccgcgaga tgggcgcggc
    10501 gcgagttggc gtgcctggcg agtggtttgg attcgccatc gaatcgaaag agacggggtc
    10561 gctaatagga gactgcgcct tgagggtcga cgagcacgaa ccttaccggg ctgaggtcgg
    10621 gttcacgctg gccagggagt accagggtag aggtctcgcc tccgaagccg tcgcgcgcgt
    10681 gttggattac gcgttcggcg ccctgggact gcaccggatc atcgccatag ctgattgcag
    10741 gaacgcgtct tccgtggcgc tgctcgagcg gctgggtatg cgtagggaag gccacttcgt
    10801 ggagaacacc tggttcaagg gaaggtgggc cgacgagtat tcgtacgctg ttctaaggca
    10861 cgaatggctc agcaagcgct caggagtatc accttaacgt cgaccgccgt cgccgcatac
    10921 gatcagtgct accccgaaag ctctcgtccc cggttccgta ccactttgcg caccatggcc
    10981 agccacagca tcctgggcca gtcaagatac cgggggtggc agttccggtc ctgggtccgt
    11041 tgattgtgct ctcctacagc gggtagcaga aggattgacc tcctgcaacc cgcgccgtaa
    11101 gcacccagcg ataacccttc agctttttgt tcttgctgat cgctgaaagc ttatagctta
    11161 tagctcgtcg tgcataaggg ccctaccttt atgcacgacg ctgtataggc ttacgggaag
    11221 cccacgatcg acttctcgga ggcgtcggtg gtcctggtgt ctgtaccggt gccgccgtcg
    11281 agggaatcgt tgccgctcac cccgtctcgg gagatcagcg cgtcgttggc actccccccg
    11341 ttgaggttgt cggtaccacc accacctgtc accttatccc ccccgccgtc gccgcgcaga
    11401 actgggtcgt cacctcccat gccatcgatg gtgtcgttgc ctgccatgcc ctttatggtg
    11461 tcattgcctg ccatgccgca gatggtgtcg gcacccaagg tgccttcgag aacgtcgttg
    11521 ccggaggttc cggttatcgt gcaggccttg aatgtggccg tgtaggtgcc cggttgggct
    11581 ccagtgaata tttcgtggtc ctgcgtcccg ccgtccgacc aggcgtcgaa cgtgtgggtg
    11641 ctgcccgcca gcgcctgcgg cgacagcgcg gtcaccctca gcttgtaacc ctcccacgag
    11701 acgaaagtcc gcggcgttgt gaacgtggtg ccgttgaggc gcaacttgag tccttgcggc
    11761 cgggactcga acgttacgtc gaccgtcttg gggggcagct tctgggtgac ggtcttggag
    11821 agcccctcag agtcggtggc ggtcagcctg atctccaggt agttttcctc ggggccggtg
    11881 gagagtaggt cttcggcgtc ggctgcggtg aaagtaaggt cgttgccggt tcctgcgaag
    11941 tgcgggtgcg tgtggtcgcc gttgtggtgt tggacgacct cccagccgag cgcactatct
    12001 ggcagttcgc catcttgtgg gtccgtcgca ctaccccgca acacgatctt ctctccgacc
    12061 gcgaagagct tgtcttgggc aggagatgag atggtcggct cgggcggccc gtttccggcg
    12121 tatatgcgca ctctcgccgt gtcctcggcg tccttggtgt cccttaccgt aagcgtggcc
    12181 gtgtgcgtac cggcttgctc gtaggtgtga ctggcggtcg gggtcgcggt ctcgaccgcc
    12241 ggtgagccgt cgccgaagtc ccagctgtag gccgccgggg cgtcgccgga gtccgggtcc
    12301 gtgctccctg agccgtcgaa actcaccgtc agcggcgcgt cgccgtattc ggggttaacg
    12361 ctcaagacgg cgtgaggcgc gcggttggct ccggcttcgg tgtaggtaat gcggcggacc
    12421 tccccaccgt tggcgtaagt ggcgtagtag agcgcctgac ccgagccgta tgggccgaag
    12481 gcaatggcga cgggaccgcc ctgatccaac ccactcgcga actccgtctg cgtgaagccg
    12541 cccccgctct tgggctcgag ctcgaagatc ttgttgcaca cgtagtcgcc gtagagatag
    12601 gagccgtcgt aggaagcggg ccacacgccg tctggcacga acgccccgcc ggtgatcgag
    12661 gagcagccgg tgtcgtggct gtactcgtga acgggaggtg tgtaaggtgc ggcgccgcag
    12721 ttcacggagc ccgcacgggc gccgttgtcg tggttgccct cgcagaggtt ccaggcgtag
    12781 tcggccccct tcttgccctg gtcgacctcc tcccaaccct cattgccgac gtcaccgatg
    12841 aagaagcgcg tgtccgaggc gttcgggtcg aaggcgaagc ggaaggggtt gcgcaacccc
    12901 gaggcgaacg tctcctggca cctcttgccg ggatcggtgc ggccagtaac gttgcagcgg
    12961 gcgctgtctg tgcccaggta ggggttgctc ggtgggatgt cgccgtcgcg ggttatgcgc
    13021 aggatcttgc ccaaaaggac gtgcgggtcg cgggaggcgt cgttgttgat ctggcagccc
    13081 gagtcgcccg cgtagtcgca cccaccatcg ccgacgctga tgtagagata gccgttcttg
    13141 ccgaagttca ggtccccgga attgtgacgg ctcggagaag ggatgttgtc gatcagcact
    13201 ttctcgctcg aagggtcgat cgtgccaccg gacattacga agcgcgagac ccggtttacg
    13261 ggatttttcg gattgtgcgt atcgtacacc ggacactcac cgaacttgtt gaaggtgtaa
    13321 tagagataga cgtagcggtt cgtcgagaag ctcgggtcta aggttacgcc gagtagccca
    13381 cgctcggagt tggatcaaac cttgccggta aggtccagtg cgggggtctg tagtagctct
    13441 ccgtccttat gcacccgtaa caggcctggc tgggtggcga taagcatgcg gccgtcgggt
    13501 gcgaaggcca ggtcgatggg gctgaaaatg ggcgtgaccg atcggtcatc gaattccgcc
    13561 gatcgcaaag tggccgtcac gccgccgttg gcgtggagga ataccagtac aacaacggcc
    13621 gacagggcca cgaccgccaa gaggacgacg tgcctgacca tacgaagatg gatctctctt
    13681 ctcatcagac ctcccacctc tcgacgcctc aaaatgcttc ccttgaaaga ccgtgcccct
    13741 gcctacctgc aacaccttct cgcccgcgct cccggccatc tcggcggccc ccttgtcgtg
    13801 ggacagcgaa aaggtgaagg cctccaccct gattttacga tgctcttaag tatcgttacc
    13861 ttgggtggcc gggcgcatcc ttcaattgag tgatttttga tgttacttgg gcgtagcttg
    13921 ccggcaacta gttgcatacg cggcttgttc cttccgagaa gtcctgctct atggacgtta
    13981 ttggggtgcc caatgcatat cggcccgtgc cgattccggc gcttccgctt ttgacagcgc
    14041 cggtttatat tcacctccgc acgcacggcc tgaccttctc cggcagccgg tagtgcgcct
    14101 ccccatcccg ctccgtgacg aacaccggcg actcatccac cagatacgtc gggaggtccg
    14161 acacctcgcc tacgccgatg ccagaggcgg cgccaggctc gtcttgacaa gcgtcccgaa
    14221 gcggcgtact atgccgtgcg ggatatatcg tcacacgatg catcgtgtga cggaacgatg
    14281 tagagggatg gtgacatggg cggcgaggat atgacggggg aacgggcgag ggatccggga
    14341 gaccttctac ccctgacgcc cgtcgtgctg aacgtccttt tggctttggc cgacggggag
    14401 cgccacggct acgggatcat gctggaggta agggagagga ccggcgggag ggtgcggctg
    14461 ggaccgggga cgctctacgg agcgatcaag cgtctgaagg agggcggtgt gatcgaggag
    14521 tcagggcaga ggcccgaccc cgccgaagcc cctgacgagc ggcgccgcta ctaccgcctc
    14581 accggcttcg gcggggaggt ccttgcggcg gagatcgaga ggctcgacgg cttggtgcgc
    14641 gccgcgcgcc gcaaaggcgc cttccccgcg ccgaacccgg agggggcgtg atggccgatc
    14701 ctgggggacc gagtagaggt gggcacgaac gcgccgtggg gacctcggag tgggtctacc
    14761 ggtcgctcct gcgggcctac ccgcgcgggc ttcgagagga gtacggcgag gagatggccc
    14821 gttgttttag ggacctgtgc cgcgagaggc tggaagtcgg cggcggcctg ggtctggcgg
    14881 cgctgtgggc ccatatcctg actgagttgc tatgcaccgc gctcaaggag aggagcacca
    14941 cgttggacag gaacacgtac cgatccgtcg tcggcgttgc gttggcgacg gcgttcatat
    15001 tgctgctgcc cttgttggcg gcaccggcct ggagcctggc cgatttcgtt gtcgccggtg
    15061 ccctcatctt cggcaccggt ctcacgtatg tgctggtagc gaggaaggcg ggcaaaatcg
    15121 cgtaccgggc cgccgtcggc gttgcgctcg cggcggcgct ccttctcgtc tggataaacc
    15181 ttgccgtcgg cgtcatcggg agcgaggacg accccgccaa cctgatgtat gtcggggtgc
    15241 tcttcgtcgg gatcgtcggc gccatcgtct cgcgcttgcg gccggatggg atggcgcgcg
    15301 cgttgctggc gacggcgctc gcacag
//
LOCUS       DATMVF010000025         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_278856, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000025 DATMVF010000000
VERSION     DATMVF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278856"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VN178_01010"
     CDS             complement(<1..516)
                     /locus_tag="VN178_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79570.1"
                     /translation="MLGRYAELKAQLPPETILLYQVGTFFETFEEDAKRISQALSIRL
                     TSREAAGEGRVPLAGVPGHALQEHLATLLRKGYTVAVAEQRQHPTKPKQFTREVTRVL
                     TPGTVIEDNVLSAGQANYLAAFVVRDGLAGIAVVEASTGEFTGTEVPEEQLPAELERW
                     SPREIVVPERTA"
     gene            complement(577..1590)
                     /locus_tag="VN178_01015"
     CDS             complement(577..1590)
                     /locus_tag="VN178_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79571.1"
                     /translation="MSTVIEFVVALVAIILGAAFFTNAIEILGGRLGMRQGAVGSLLA
                     AVGTALPESMIAIVAILEPVLTGRASEEGALIGIGAILGAPFMLATLAMFVVGISALS
                     FRRRRDQGMRLHLDAATVGRDVGFFLVFFTLAAGVGLVELPLYLKVAFALVLAIGYGH
                     YVRRTLFSGEHLEEVPKRLLFLPRRQKPPLFAVVFQTLASLGVIVAGAHFFVDAVEHA
                     AAGLGLPAGLIALILAPLATELPEKFNSIFWIREGKDTLALGNITGAMMFQSTVPVAF
                     GVVFTPWNLAPLDLFAVVLALVSGGLVYLSLRLSGKLRAGRLMLGGLFYLAFVVGAVI
                     VVV"
     gene            1916..>2284
                     /locus_tag="VN178_01020"
     CDS             1916..>2284
                     /locus_tag="VN178_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWS79572.1"
                     /translation="MSPKPVILDVDPGHDDAVALMLACSHPDLDLLAVTTVAGNVPLA
                     KTTRNTLRVLSLIGCTDVPVGVGASEPLERPLHTAEDIHGKSGLDGSEEIPDAGFGPD
                     ERGAVVLIADTLRASPEPVTL"
BASE COUNT          432 a          753 c          720 g          379 t
ORIGIN      
        1 cgcagttcgc tcggggacga cgatctcgcg cggcgaccaa cgctctagct cggcggggag
       61 ctgctcctca gggacctcgg tgcccgtgaa ctctcccgtc gaagcctcga cgacggctat
      121 gccggccagg ccgtccctga cgacgaacgc ggccaggtag ttggcctggc cggcggagag
      181 gacattgtcc tctatgaccg tgcctggtgt cagaactcgg gtgacctccc gcgtaaactg
      241 cttgggtttc gtcgggtgct gccgttgctc ggcgacggcg acggtgtaac cctttctgag
      301 cagggtggcc agatgctcct ggagggcgtg tcccgggacc ccggccaacg ggacgcggcc
      361 ctcgccggcc gcctcgcggc tcgtcagccg gatcgagagc gcctgcgaga tcctcttggc
      421 gtcctcctcg aaggtctcga agaacgtccc gacctggtac aggaggatcg tctccggcgg
      481 aagttgcgct ttcagctccg cgtaacgtcc aagcataggg ctattctacg gcccgcaccc
      541 cgcttcgtca gccccaaata aggcccctgt gccggatcat acaaccacta tcaccgcgcc
      601 gacgacgaaa gcaaggtaga acaggccacc gagcatcagc cttccggccc gcagtttccc
      661 ggagagcctg agcgagaggt agacgagccc tcccgacacc agggcgagca ccacggcgaa
      721 gaggtcgaga ggggcgaggt tccacggcgt gaaaacgacg ccgaaagcaa cgggtacggt
      781 gctctggaac atcatcgcgc ccgtaatgtt gccgagtgca agggtgtcct taccttccct
      841 gatccagaat atggagttga atttctcggg gagctcggtc gccaggggcg ccagtatcag
      901 ggctatgagg ccggcgggca gccccaggcc cgcggcggcg tgctcgaccg cgtcgacgaa
      961 gaagtgcgcg cccgcaacga tgaccccgag gctcgccagc gtctggaaga cgacggcgaa
     1021 gagcggcggc ttctgcctgc gcggcaggaa tagaagcctc ttcggcacct cctcgagatg
     1081 ctcgccggag aatagcgttc gtctgacgta gtggccgtag ccgatggcca gcacgagggc
     1141 gaaggcgacc tttaagtaca gcggcaactc caccagtccg acgcccgcgg ccaaggtgaa
     1201 gaagaccagg aagaacccga cgtccctgcc gacggtcgcc gcgtcgaggt gcagtctcat
     1261 cccctggtct ctgcgtctcc taaaggagag cgccgagatt ccgacgacga acatggcgag
     1321 ggtcgcgagc atgaacggtg ccccgagtat cgctcctatg ccgatcaggg ctccttcttc
     1381 cgatgctctg cccgtaagga cgggctcgag gatggcgacg atggcgatca tgctctcggg
     1441 caaagcggtg cccacggctg cgaggaggct tcccacggcc ccctggcgca tcccgaggcg
     1501 tcctcccagt atctctatgg cgtttgtaaa gaaggcggcc cccaaaatga tcgcaaccag
     1561 cgccaccacg aactctatga ccgtcgacaa tacccatctc ctctctcaga cgggccgcga
     1621 gacacgaaaa agcgagacca tcgcggcctg tttacctacc gatggtctcg ctctgtgcgc
     1681 cttggcgctg gtaaccggcc gagagcctcg ctccgtaatg acgaccggtc cccccgcaac
     1741 agcgggtggc tactccccaa tttcttgtta tcttacccga tcgaatcctg tcgacggtga
     1801 gacttttcac cgggacgagg acccccgccg caccgctcgt cgactactcg cgctgtacgc
     1861 ggggctcgga aagagataac ttagcaacca gccggttgcg tagagaggag cgagcgtgag
     1921 tcccaagcct gtgatcctgg atgtagaccc gggccacgac gacgctgtgg ccctgatgct
     1981 ggcctgcagc caccctgacc tggacctgct cgccgtcacg accgttgcgg gaaacgtgcc
     2041 tctagcaaag actacgcgca acaccctgcg ggtgctctct ctcatcggat gtacggacgt
     2101 gcctgtgggc gttggcgctt cggaacccct ggaaaggccg ctccacacgg ccgaagacat
     2161 tcacggcaag agcgggctgg acgggtccga ggagatcccc gacgccgggt tcgggcccga
     2221 cgaaaggggg gctgtggtcc tgatcgcgga caccctaagg gcctcgcctg aacccgtcac
     2281 cctc
//
LOCUS       DATMVF010000026         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_2661, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000026 DATMVF010000000
VERSION     DATMVF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="coaE"
                     /locus_tag="VN178_01025"
     CDS             complement(<1..543)
                     /gene="coaE"
                     /locus_tag="VN178_01025"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWS79573.1"
                     /translation="MEGPVTVAVTGPFASGKSALVRFLGELPGTETASADEIVHHLLE
                     NDRQTISRIVDRFGEDVGGSDGIDRKALGRKVFGDAGALRDLEEILHPLVRSETDRRI
                     EASVAELFVVEIPLLFETGRDGDFDFTVAVTVPEERRRAWAAERGLDGAALRAVETRQ
                     LSGEEKARRASLTIQNDGDLD"
     gene            complement(570..1787)
                     /gene="rpsA"
                     /locus_tag="VN178_01030"
     CDS             complement(570..1787)
                     /gene="rpsA"
                     /locus_tag="VN178_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWS79574.1"
                     /translation="MRDFFREENGEVVPVDESVLIDFKDGDIVEGEVVRIDRDEVLVD
                     IGYKSEGLVPSNELTIRKGADPHDVVELGQRLEALVMQKEDADGRLILSAKRAAFERA
                     WNRIEDAYNDQRTVEGPVIEVVKGGLILDIGLRGFLPASLVDIRRVRNLDSFLGQRLE
                     CKVIELNRSRNNVVLSRRAVLEEERKEERERILTSLEEGMIVKGTVSNLVDFGAFVDL
                     EGIDGLIHISELSWQHVDHPSEVVEVGEEVEVKVLEVDRDRERISLGLKQTRKDPWQE
                     IVERVTVGEQIHGRVTKLVSFGAFVEVADGVEGLIHISELADHHVETPDEIVRSGDEV
                     DARIIDVDARRRRLSLSLRPKREDREERPRREERPRREPEQRSTPDRRSSEGSGLRSG
                     AFDALADLDLEER"
     gene            complement(1942..>4030)
                     /locus_tag="VN178_01035"
     CDS             complement(1942..>4030)
                     /locus_tag="VN178_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWS79575.1"
                     /translation="MQLVDGKVKVLRTTRGVSETKTYGRDEVLEEYGVTPEQIPDYKA
                     LTGDSSDNIPGVRGIGPKGAANLLKEFGTLDGVYENLENISAKGTRTKLEEGRESAFM
                     SLELAKMRFDVPVKFEPENLHFEGVSPEIRDITRRYEFRTLESRLTELPVAGGERLPP
                     LERLEVSVSEDPVELSFEPVAASPLGDGRWCVAVSAGEVRVADGLPDVPLKVHDSKGL
                     GVRVADFDTYLAAYLVKPGLGSYELETLAAERGMAEVEVSHEDRTVAEAARRAALVYA
                     LSPRLEEELEAMGLARLYYDVELPLAGVLEEIEETGMPVDAATLEEVGEELEGRISDV
                     EQHIYEDVGHPFNIGSPKQLGEVLFEEMGIPPVRKTKTGYSTDAKVLQQLAIEHPVAD
                     RIIEYRELTKLRGTYIEGLLKLITEEGRIHSTLHQTRAATGRLSSDSPNLQNIPIRTQ
                     LGARIRDAFTASPGRKLVVADYSQIELRILAHMTEEPALVESFMKGEDIHTRTASEVF
                     DVRMESVTPELRRRAKMVNFGILYGISGFGLATRLGNVHPAEAERYIKRYFERYPRVT
                     EFMQETLEEAEELGYSTTLFGRRRYVPELRNPNKNVRKLGERIAFNARVQGTAADIIK
                     VAMVDLAPRLGPLGADMIMQVHDELVFDVDEDRVGEVAGLAIDRMVAAYDLKPPLEVE
                     AKAGDQWGRVSPL"
BASE COUNT          634 a         1373 c         1221 g          802 t
ORIGIN      
        1 atccaggtct ccgtcatttt gtatcgtcag gctggcccgc ctggccttct cttctccgga
       61 gagctgccgc gtctccacgg cccgcagcgc cgccccatcc aagccccgct ccgccgccca
      121 cgcgcggcgc ctctcttcag gcacagtgac ggccaccgtg aagtcgaagt ctccgtcccg
      181 tcccgtctcg aacagcagcg gtatctccac gacgaagagc tccgccacgg aagcctcgat
      241 acgcctgtcg gtctcgctac gcaccagggg atgcagtatc tcttcgaggt cccgcagcgc
      301 cccggcgtca ccgaacacct ttcggcccag ggctttgcgg tctataccgt ccgatcctcc
      361 gacatcttcc ccgaagcgat cgacgatcct ggagatagtc tgcctgtcgt tctccaggag
      421 gtggtgcacg atctcatcgg ccgaggccgt ctccgtgccg ggcagttcac ccaggaagcg
      481 gaccagcgcg ctcttgcccg acgcgaaagg gccggtgacg gctacggtca ccggcccttc
      541 cacggaccct ctctgtaggg agtccttgtc tatctctcct cgaggtccag atcggccagc
      601 gcgtcgaagg cgccggagcg gaggccgctc ccctcgctgc tcctgcggtc cggggtgctg
      661 cgctgctcgg gctctctgcg cgggcgctcc tcccggcggg gacgctcctc gcggtcctcg
      721 cgcttcgggc gcaggctcag ggagagcctg cgacggcggg cgtcgacgtc gatgatgcgg
      781 gcgtcgacct catcgccgct gcgcacgatc tcgtccggtg tttcgacgtg atggtcggcc
      841 agctcggaga tgtggatcag accctccacc ccgtcggcga cctccacgaa ggccccgaag
      901 gagacgagct tggtaaccct accgtggatc tgctccccga ccgttacccg ctcgacgatc
      961 tcctgccagg gatccttcct ggtctgcttg aggccgagcg agatgcgctc gcggtcgcgg
     1021 tcgacttcca gaaccttgac ctcgacctcc tcgccgacct cgacgacctc gctcgggtgg
     1081 tccacgtgct gccaggaaag ctcggagatg tggatcagac cgtcgatgcc ttcgaggtcg
     1141 acgaaggccc cgaaatccac gaggttggag acggtgccct tgacgatcat gccctcctcg
     1201 agcgaggtca ggatgcgctc gcgctcctcc ttgcgctcct cctcgagcac ggcgcgcctg
     1261 ctgaggacca cgttgttgcg gctccggttc aactcgatga ccttgcactc cagcctctgg
     1321 cccaagaacg agtcgaggtt ccttacgcgc ctgatgtcga cgaggctggc cggcaggaag
     1381 ccgcgcagcc cgatgtcgag gatcaggcct cccttaacca cctcgatcac gggcccctcg
     1441 acggtgcgct ggtcgttgta ggcatcctcg atgcggttcc aggcccgctc gaatgcggcc
     1501 cgcttggccg aaagaataag ccgcccgtcg gcgtcctcct tctgcatgac gagggcttcg
     1561 aggcgctggc cgagctcgac gacgtcgtgg gggtctgcgc ccttgcggat ggtgagctcg
     1621 ttggagggaa cgaggccctc cgacttgtag cctatgtcga cgagtacctc gtccctatct
     1681 atccggacga cctcgccctc gacgatgtcc ccgtccttga aatctatgag gacgctctcg
     1741 tcgacgggga cgacctcccc gttctcctcc cggaagaaat ctctcatggt cagttcccgg
     1801 gacggtttct gtgtggtgtc agtcatgaaa cagtagcttt cctttacatc gcttgacggg
     1861 tgcagattat acagcaatta tggagctgtc agctgtcagc caaaaacaaa aagctgaagg
     1921 ctgagggctg aaagctgaag gctataaagg acttacccgt ccccactgat ctccggcctt
     1981 ggcctcgact tcgagcggcg gcttgaggtc gtaggccgcg accatcctgt ctatggccag
     2041 gccggcgacc tccccgacac ggtcctcgtc gacgtcgaag acgagctcgt cgtggacctg
     2101 catgatcata tccgccccga gaggacccaa cctcggcgcg aggtccacca tcgctacctt
     2161 gataatatcc gcggccgtac cctggacgcg ggcgttgaac gcgatcctct cccctagctt
     2221 cctcacgttc ttgttggggt tcctcagctc gggcacgtaa cgccgccgcc cgaagagcgt
     2281 cgtggagtac cccagctctt cggcctcctc cagggtctcc tgcataaatt cggtcaccct
     2341 cgggtagcgc tcgaagtagc gcttgatgta ccgttcggcc tcggccgggt gcacgttccc
     2401 cagcctcgtc gcgagcccga accccgagat gccgtagagg ataccgaagt tgaccatctt
     2461 cgcccggcgc ctgagctccg gggtcacgct ctccatgcgc acgtcgaaga cctccgaggc
     2521 ggtgcgcgtg tgtatgtcct cgcccttcat gaacgactcc acgagcgcgg gctcttcggt
     2581 catgtgggcc aggatcctga gctctatctg ggagtaatcg gcgaccacga gcttgcggcc
     2641 cggcgaagcg gtgaacgcgt ccctgatacg ggcgcctagc tgcgtgcgga tggggatgtt
     2701 ctgcaggttg ggcgagtcac tggagagacg gcccgtggca gcccgcgtct ggtgtaatgt
     2761 gctgtgtatg cggccttctt cggtgatgag cttgaggagc ccctctatgt aggtgcccct
     2821 gagcttggtc agctcgcggt actcgattat gcggtcggcc acgggatgct ctatggcgag
     2881 ctgctgcaag actttggcgt cggtggagta gccggtcttg gtctttctga cgggtggtat
     2941 gcccatctcc tcgaagagga cctcaccgag ttgcttgggc gacccgatat tgaaggggtg
     3001 gccgacgtcc tcgtagatgt gttgctccac gtccgagata cggccttcca actcctctcc
     3061 gacctcctcg agggtggcgg catcgacggg catgcctgtc tcttctatct cctcgagcac
     3121 gccggcgagc gggagctcca cgtcgtagta gagcctggct agtcccatgg cctccaactc
     3181 ctcctcgagc ctcggtgaga gcgcgtaaac cagcgcggcc ctcctcgcgg cctcggcgac
     3241 cgtcctgtcc tcgtggctga cctcgacctc ggccatcccc ctctccgcgg ccagagtctc
     3301 cagctcgtag ctccccagcc ctggtttgac caggtacgcg gcgagatagg tgtcgaaatc
     3361 cgccacccgc acaccgagac ccttggagtc atgtaccttg agggggacgt cgggcaaacc
     3421 gtcggcgacg cgcacttcgc ccgcagagac ggcgacgcac cagcgcccat ccccgagcgg
     3481 tgaggccgcc accggttcga aggacaactc gacgggatct tcggagacgc tcacctccag
     3541 gcgctcgagc ggcgggagtc tctcacctcc tgcgacgggc agctcggtga ggcgcgactc
     3601 cagggtgcgg aactcgtagc gcctggtgat gtcccggatc tcgggcgaga cgccctcgaa
     3661 atggaggttc tcgggctcga acttcacggg cacgtcgaaa cgcatcttcg cgagctccag
     3721 cgacatgaac gcgctctccc tgccctcttc cagcttcgtg cgggtgcctt tcgccgagat
     3781 gttctccagg ttctcataga cgccatcaag ggtgccgaac tctttcagca ggttcgccgc
     3841 gcccttgggg cctatgcccc tgacaccagg gatgttgtcc gaggaatctc ccgtcagggc
     3901 cttgtagtcc gggatctgct ccggtgtcac gccgtactcc tcgaggacct cgtccctgcc
     3961 gtacgtcttg gtctccgaga cgccgcgggt ggtacgcaac accttgacct ttccgtccac
     4021 gagctgcatg
//
LOCUS       DATMVF010000027         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_599703, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000027 DATMVF010000000
VERSION     DATMVF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599703"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VN178_01040"
     CDS             <1..562
                     /locus_tag="VN178_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS79576.1"
                     /translation="MHENPDYLFEAVKAGAAGYVLKDASGERLLGAVRRTLDGESPLD
                     QELAMRLLKRLARERRGEGSGGRSGGTSREPPEDAPAGHREVDGPLATRPAATHSGAH
                     EDGPGGSAQSRCAEQMESLTPREVEVLRLLSQGQTNPQIAKNLLFSVSTVKAQVRSIL
                     SKLGVSDRTQAAVRAIEARHTISGID"
     gene            complement(769..1914)
                     /locus_tag="VN178_01045"
     CDS             complement(769..1914)
                     /locus_tag="VN178_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWS79577.1"
                     /translation="MRRERWARRPRGEGREEKSASGGHVTPILISLRIWISIVVAGFA
                     LFALLLYAAPTVPAVALGGVALAIVLSFPVRALSHIMPRGLAILLTVLAMLGLISLGF
                     FFLVPLLVEQLSTLVRNTPRIANSANQLLLDVINALNERQLVPGSDPEEFGRRLVTDL
                     FDRAQNLTENLLRSILGFIPRVFNFGVTLFGILFVAIYLLVDVRKVKATFLRAVPAHY
                     RHDARDLWDASGESLSRYLGGLVFVVMIQGVLAAAALYLLGVRYAILLGVWVSLTAII
                     PYLGAFLGGIPAVIVALVFGSPNFESSTTTAILTIVAYVLIQQLEGNFLTPRIQGTAL
                     QVHPILVLLAVIAGGEVAGLAGVVFAVPTLAVFRVLFDFFRVRLRRK"
     gene            complement(1986..2840)
                     /locus_tag="VN178_01050"
     CDS             complement(1986..2840)
                     /locus_tag="VN178_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HWS79578.1"
                     /translation="MVEASFLASFLGGLLSLLSPCSALLLPAFFAYAFQSRGVLVGRT
                     AVFYLGLCATLVPLGMGISAVSSLVYGHRSTLITVSGLVIVALGVIQALGGGFAFGPI
                     ERLRGGIRGSSLLATFALGAVYGFAGFCSGPILGAILTVAATSGSALRGAGLLATYAA
                     GMAAPLFIIALLWDRLDLGRRRWLRGREIPLGSLRLHTTNLISGLMFVAIGVLFIVSE
                     GTSGLEGIYASRGATDLAFSVERWAGSFEEGALLSITFAGLGLLFAGFFVYRFARRRD
                     KKPVERGD"
     gene            complement(2833..>3114)
                     /locus_tag="VN178_01055"
     CDS             complement(2833..>3114)
                     /locus_tag="VN178_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWS79579.1"
                     /translation="YHDLLFESQFSGFGDENLIGLAERAGFDTRQFERDYENARYEPL
                     VRADFQKGLNAGVNGTPTFFINGEMLVGLQPIGVFERAIEDARREAEGG"
BASE COUNT          669 a          938 c         1053 g          454 t
ORIGIN      
        1 catgcacgag aaccccgact acctctttga ggctgtaaag gcaggggcag cgggctacgt
       61 gctcaaggac gcttcagggg agagactctt gggtgcagta agaagaacgc ttgatggaga
      121 gtccccgctc gaccaggagc tcgcgatgag actgctcaaa aggctggcca gggagaggcg
      181 tggagagggt agtgggggac gatccggagg aacgtccaga gagccgccag aggatgcacc
      241 ggcgggtcac agagaggtcg acggtcccct ggcaacccgg ccagcagcca cccattccgg
      301 tgcgcatgag gacgggccag gaggatccgc gcagtcgaga tgcgcggagc agatggagag
      361 cctcacgccg agagaggtcg aagtattgag gctgctgagc caggggcaga ccaaccccca
      421 gatcgccaag aacttgctct tcagcgtgag caccgtaaag gcgcaggtgc ggagcatcct
      481 ctccaagctc ggagtatccg accgcaccca ggccgccgtc cgcgccatcg aggccagaca
      541 tacgatctcg ggcatcgact agagaagcat ttcagcacgc tccagccttc ggcttccgct
      601 tgtcagcacg gcggcttcgc cgccttgtca gcacgctgcg cccggcttcg ccaggcttcg
      661 ctttcagcat gggcgtgcac gcacgaaagc tgaattgctg acaagcgagg gcgtcagccc
      721 gagcgtgctg aaatgctgac aagcctgcga agcaggcgtg ctgaaatgct actttcggcg
      781 aagccgaact ctgaagaagt cgaacagaac gcgaaatacg gcgagggtgg gaacggcgaa
      841 gactactccg gcgaggcctg cgacttcacc gccggcgata acggcgagaa ggacgaggat
      901 ggggtgcacc tggagggccg tgccctggat gcggggggtc aggaagttgc cttctagctg
      961 ttggatcaag acgtatgcca cgatcgtcag gatggccgtg gtcgtcgagg actcgaagtt
     1021 gggagacccg aagacgaggg ccactatgac cgcgggtata ccccccagga acgcgccgag
     1081 gtaggggatg atcgccgtta gagagaccca cacgccgagc aggatggcat aacgcacgcc
     1141 gaggagatat agtgccgcag cggccaggac gccctggatc atgaccacga aaacgaggcc
     1201 gccgaggtag cgcgagagcg actcgccgga ggcgtcccac aggtcgcggg cgtcgtggcg
     1261 gtagtgggcc ggtacggccc tcaggaaagt cgcctttacc ttccgcacgt ccaccaggag
     1321 gtagatcgcg acgaagagta tgccgaagag cgttaccccg aagttgaaga ccctggggat
     1381 aaacccgagg atgctcctga gcaggttctc ggtaaggttc tgggcgcggt cgaagaggtc
     1441 ggtgaccagc ctcctgccga actcctcggg gtcggagccg ggtacgagct ggcgttcgtt
     1501 cagcgcgttt atgacgtcga gcaagagttg gttggcgctg ttggcgatgc ggggagtgtt
     1561 cctaacgagc gtgcttaact gctctaccag gagcggcacg aggaagaaga aaccaaggga
     1621 gatcaagccg agcattgcca gaacggtcag caggatcgcg agccccctcg gcatgatgtg
     1681 cgagagggcc cgcaccggga acgagagcac gatggccagc gccaccccac ccagcgccac
     1741 ggcgggaacg gtcggcgccg cgtacaaaag gagcgcgaac agggcgaacc ccgccacgac
     1801 gatgctgatc cagatcctca aagagatgag gattggggtc acgtggccgc cggacgccga
     1861 cttctcctcc cgtccctcgc cccgaggcct ccgggcccac ctttctctcc tcatcgtctc
     1921 cggttctctc cgagtacgcg gttacgaatc tgattctacg gcaagagtag ccccatcgga
     1981 gcggcctaat ccccgcgctc cacgggcttc ttgtcgcggc gtcgcgcgaa cctgtagacg
     2041 aagaagccgg cgaacagcag gccgagaccc gcgaatgtta tggagaggag tgcgccctct
     2101 tcgaagctcc ccgcccaccg ctcgacggag aaggcgagat ccgtcgcacc gcgcgaggcg
     2161 tagatgcctt cgaggccgga ggtgccctcg gagacgataa acaagacccc tatcgcgacg
     2221 aacatcaggc cagagatgag gtttgtcgtg tgcaggcgga gggatccaag cgggatctca
     2281 cgccccctca accagcggcg gcgcccgagg tcgaggcggt cccagaggag ggcaatgatg
     2341 aagagagggg cggccatgcc ggcggcgtac gtggcgagga ggccggcgcc ccggagggcc
     2401 gaacccgatg tcgcggcgac ggtgagtatc gcgccgagga tgggtcccga gcagaagccc
     2461 gcgaaaccgt agacggcgcc gagagcgaag gttgccagca gggaactacc acggatccca
     2521 ccccgcaggc gttcgatggg cccgaacgcg aatccgccgc ccagagcctg gatcacgcct
     2581 aaagcaacta ttacgagacc cgagacggtg ataagtgtgc tcctgtgccc gtagacgagc
     2641 gagctgaccg cggagatgcc catgccgagc ggtacgagcg tggcgcagag cccgaggtag
     2701 aacacagccg tcctcccgac gagcacgccg cgcgactgga aggcgtaggc gaagaacgcg
     2761 ggcagcaaca acgcgctgca cgggctgagg agagagagca ggcccccgag gaacgaggcc
     2821 agaaacgagg cctcaaccac cctcggcctc ccgcctggca tcctctatgg ctctttcgaa
     2881 gaccccgata ggctgcaagc cgaccagcat ctcgccgttt atgaagaagg tcggtgtccc
     2941 gttgacaccg gcgttcaggc ctttctggaa atcagcgcgc acgagtggtt cgtagcgggc
     3001 gttctcgtaa tctctctcga actgccgggt gtcgaagcct gccctctcgg ccagcccgat
     3061 gaggttctcg tcgccgaagc ccgagaactg gctttcaaag agcaggtcgt ggta
//
LOCUS       DATMVF010000028        12770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_187930, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000028 DATMVF010000000
VERSION     DATMVF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187930"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1512)
                     /gene="hydA"
                     /locus_tag="VN178_01060"
     CDS             complement(106..1512)
                     /gene="hydA"
                     /locus_tag="VN178_01060"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWS79580.1"
                     /translation="MSGLLFSGGTVVSAEGSYRADVLVEDEKIVAVGANLDPDGAETV
                     DASGKLVMPGFIDAHTHMDMPFGGTVTADDWATGTEAAAAGGTTMIIDFALQEAGGAL
                     AGAFDAWTEKARDKAVIDYSFHVAITDLRDDIKAELPDLAEKGVASVKIFMAYKGTPL
                     YTEDEDLFEALQLSKDAGVLVMVHAENGDAIAKLQQQALSRGDTAPRFHALTRPEPVE
                     AEATNRAIRLAEVAGAPILVVHVSCAPALEAIHLAHERGQTVYAETCPQYFAFEYEDL
                     DREGFEGAKYVCSPPLRDPENRPALWNGLKFGDLQIFGSDHCSFNYREQKELGKDDFT
                     LIPNGLPGAEERAMTLWTLGVREGKLSENQFVAVLSTNQARIYGAYPRKGTLAPGADA
                     DIVLWDPELSTTATVENRHGNVDYTPYEGMVFHGGPAEVYVRGGLVYKDGEVVGQRGS
                     GRFVERSFTATEGLEVRV"
     gene            complement(1723..2808)
                     /locus_tag="VN178_01065"
     CDS             complement(1723..2808)
                     /locus_tag="VN178_01065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS79581.1"
                     /translation="MRVAIIGAGIVGGSVAYRLSEGGAEVVMIDGAEPGSGTTSTSFA
                     WVNANNKLPRDYFELNVAGMREHERLRDEIGDGWLHPTGNLIWAANDELANLEERVER
                     LRSWSYAAEMLPAATVNGKLEPGAALPEIRIAHFPEESWVDAPALTRRLVEAAVRNGA
                     HERFGDAVRGIEVEGKGVTLRLEDGEVHADAVVNATGAKAASVAEMVGRELPLDVFPG
                     LLVRVAVLDEPLRHLMHTPRINVRPDGDGYVLLHHDSVDELLTDDFAGTEDPLCAELL
                     ERARLVLPALGEAEVVEARFGMRPVPADGHSCVGALSEIPGYYEAVTHSGVTLGPLIG
                     RLLACEILTGEIDPLIAPFRADRFPAT"
     gene            complement(2960..3904)
                     /locus_tag="VN178_01070"
     CDS             complement(2960..3904)
                     /locus_tag="VN178_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS79582.1"
                     /translation="MGWRNGGYASVLLVAVGAAMWGTDGILRVPLLKVALPSQIVLLE
                     HLVLLLYSVPAVVLGWRFFRGLGTAQWVALLIIGWGGSALATLLFTTAFAVGNPTVVI
                     LLQKTQPLFAIALAAILLRERLGWAYWPCFAVAMVGAYLISFGDLSPFMALGSAEALA
                     AFLALGAALLWGSSTVLGRLVLKDMPFNTLTGARLLIAVPLLAGIVIAQGSLDGLGTG
                     FAAEPGRVILLALIPGLLALLLYYRGLTGTRASYATLAELAFPATAVVLNWAFLGVGV
                     SANQVLGFVLLWGAVFVLGYLNARSPAPDPSAPAIGTG"
     gene            complement(3908..4912)
                     /locus_tag="VN178_01075"
     CDS             complement(3908..4912)
                     /locus_tag="VN178_01075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWS79583.1"
                     /translation="MKTTGTVGYAAMFEQFHPTDLLNWSKTAEESGFGSVMASDHFHP
                     WTPDQGQSAFVWSWMGALGATTNLRFGTGVTPPGFRYHPAIIAQGAATLEAMYPGRFW
                     LGLGAGEALNEHIVGDYWPEAPTRLRILMEAIEVIRRLFTGKVVKYSGEHIRLESARL
                     YTMPETPPPIYVATAGPIQSRRTGEFCDGIITVGAPDEKIKNLMEKFEEGARRADKDP
                     SGMPRMIQLHVSWAEDQEEAEQQAVREWPNGGMPFPKADIRNPEDFAAMARQVSVENF
                     KNRVLMSSDLDEHLEHVQHYVDLGFDEVYIHNVGRNQEEFIRAYGERVIPKLEWPEKA
                     "
     gene            complement(5123..6301)
                     /locus_tag="VN178_01080"
     CDS             complement(5123..6301)
                     /locus_tag="VN178_01080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS79584.1"
                     /translation="MMELRKYRDGDMDALTRLGVAAFGGSISDWEGNFDPAQNARLDL
                     EQVHVIEEDGEVRASTTVLPLESYVEGEWRPMGGIAAVMAHPAYRRRGYAGKLMRAVI
                     RDMRERDVALSLLSPFAHAFYRVFGYELVTEEIEYRLKPADLPTSPEQSHLRAYREED
                     LSSLMELYTEEAKGHTLSARRSEGHWKTTLSKKDADVAVYERDGDVEGYILYKTSGWQ
                     ERDPRRTLSVEELVAATQRARAALYSFMAGLDPLVYGIKHYTPRGEPLHPYLKSSHVE
                     AKIEPDQMLRLVDVEVALGYLGRTPQAPLVLDVSDDVIPENSGEYTLAEGKVIRGTEA
                     GERVSLDVRQLAQLYAGYLPAGGLAKHGLIQASSPEALDLLEAIFPLGDPWLYGPDHF
                     "
     gene            complement(6354..6920)
                     /locus_tag="VN178_01085"
     CDS             complement(6354..6920)
                     /locus_tag="VN178_01085"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWS79585.1"
                     /translation="MTTLGYAILGLLSREDLSGYDLTRRMAGRVGYFWSARHSQIYPE
                     LAKLEDGGYVAHNVVEQKERPDKKVYEITAEGLDALREWVTRPPVPRPTRDELTLKTY
                     SVWLADEEKAIRLFREEELRHEEQLAHYEGLRTWMEEEWREDLTSTDSPEFASYATLR
                     RGISYEREYAEWCRWMADSIEGRKRNDG"
     gene            7024..7527
                     /locus_tag="VN178_01090"
     CDS             7024..7527
                     /locus_tag="VN178_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79586.1"
                     /translation="MCGWSYVGHGLRRGAGGREFLGYEVEKMSPGARISAALSVLVPV
                     GLSGVFLVAFVPSLWWIFTTYFWIAFPALGLLTSGLTALGEGRPVRVSEEERERELLE
                     AIRESGGISAAAAAARTSLTVAEADGMLRALAENGHLEVIARGGAIFYALWGNEWETA
                     RRIEGSA"
     gene            7579..8085
                     /locus_tag="VN178_01095"
     CDS             7579..8085
                     /locus_tag="VN178_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HWS79587.1"
                     /translation="MAQVVDGRYTARMDEPFAVFIIGMRINNLFAVRKWLPTIRAMGP
                     MLKELYQHPEKGFLGGEFFLYWRGPALVQYWRSFEDLERFARNPDDPHLPAWQRFNRT
                     VGSDGSVGIFHETYIVERGNYEAIYGNMPVFGLAKATEHVRAVGGRETARRRLMRGEN
                     EPAVPSPE"
     gene            complement(8296..9750)
                     /locus_tag="VN178_01100"
     CDS             complement(8296..9750)
                     /locus_tag="VN178_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HWS79588.1"
                     /translation="MSALNLRVIANALAALVAAVGAVMLVPGAYSMLTGPNEGWSFWI
                     PATTALVLGLALFFLTRSRHNRYVSRQNIFLMVVVCWLGVVLVGAAPFLLAGVMGPVD
                     AFFNSMAGFTTTGAATVTPEELSPALLLWRSLSQWIGGLGIIVLFVAIAPLAGFGATQ
                     LYTAEAATPVQERITPRIRDTVRVLAFVYGGLTLGGVVVLHLVGMATFDAVNYALSTV
                     STGGFSTDSDSVAAFDSWAVELAITAGMVLAGTNFAIYYIAARRGLGRAVGNAELVTY
                     LGVIFASTLLITADLRVSDYENSLAIAFREALFQSASLTTGAGFSTTDWSVWPPFSVA
                     LLVLIMAVGGCAGSTAGGMKAIRAYLLTRHAAQEMFRLVHPRAVTPLVLGDRVLPERI
                     RVGVLSFFFVYVATVAVGTLLIALHQVPMGQAFGSVFSCVNIAGTFPGAAGTSQFYAD
                     LPATAKSTLAILMLLGRLELFTVLVLLSPAFWRA"
     gene            complement(9847..11394)
                     /locus_tag="VN178_01105"
     CDS             complement(9847..11394)
                     /locus_tag="VN178_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS79589.1"
                     /translation="MRVRVGFPLRSEGHLGRAVALASRHPSYRVEGVNGSARVCVDLD
                     LPSDWRLLDDFSGLLRGEVDTEYAADGASLSADELYGGLRCFLRKQRSGRTAREWCTP
                     ISLADKQLFPCRQIRVYDNDHLTANSWYAFGKMDEEAVFEVDKDAITERVLSDLGPCV
                     RCPILDLDSTAEIVARLPERIDPSRDNSWGYREVGDLSGWRVVKTREGREEADLQEDR
                     EGFIERLARSVRSGGAGVAAASGAAPGSRNVPATRYEDVGGMRETIDLVRESVELPIT
                     HPEIFRKLHIRPHKGILFYGPPGTGKTLLARAVAHESGAHFVAVSGPEILNKFWGQSE
                     AKLRSIFNEARAKAPAIILFDEIDSFASSRDMMSESFEATLVSQLLSLMDGLNNLGRV
                     CVIATTNRPGALDPALRRPGRFDHEIEIGLPDAEARRHILGIHTREMPTDPDLDLDQI
                     AGLTGSYSGADLEALCREAALVCMRRTINLRDFEKRIASHQLSALSVTTYDFRTAAKR
                     IGPSIRR"
     gene            11520..12050
                     /locus_tag="VN178_01110"
     CDS             11520..12050
                     /locus_tag="VN178_01110"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWS79590.1"
                     /translation="MSEQDSNPPVSTGGGDDGTTTLLGSGRMGKNDARIVALGDIDEA
                     SSFLGLARAEADEEVGGLLIRLQRLLYRVMGDLAMPKEDNVVDEDDLKHLDDALAEWR
                     GRTRIPREFVIPGESRRGALLDVARSVARRAERSLVAAGYANEHPYAVRVVNRLSDLL
                     FVLARNADGEKTLSKG"
     gene            complement(12087..>12770)
                     /locus_tag="VN178_01115"
     CDS             complement(12087..>12770)
                     /locus_tag="VN178_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWS79591.1"
                     /translation="NSAVTARDFAGTLAVEIKYNLLSPWARMAKRVLDLGATVLGGAL
                     VLPLLLVIALLVYVESGRPIFYTDWRMGKDGVPFPCIKFRTMVPGAEAVLELMLAEDA
                     GLREEYVKYHKLRVDPRVTRVGRFLRRTSLDELPQLWNVLRGEMSLVGPRPYLPRESG
                     DIGDAREEILRVPPGVTGPWQVSGRNHAFFTDRIGMDIHYVRDWSVWLDLVLLARTFR
                     ALFVDKGAY"
BASE COUNT         2297 a         4104 c         4008 g         2361 t
ORIGIN      
        1 ccttgaagcc ctcgccgcgc acgagctcga gcggctgctc gtagtagagc ggcgtccacg
       61 aaggcagcgc cgccttgtgc cgccgaaata gctcatccgg gtttgtcata cccgcacctc
      121 cagtccctcc gtcgccgtga agctccgttc gacgaacctc cccgaacccc gctggccgac
      181 gacctcgccg tccttgtaaa ccagtccgcc acgcacgtac acctccgccg ggccgccatg
      241 aaacaccatc ccctcgtagg gcgtgtagtc cacgttaccg tgacggttct cgaccgtggc
      301 cgtcgtggag agctcagggt cccacagtac gatgtccgcg tcggcgccgg gggccagggt
      361 tccctttctc gggtacgcgc cgtagatgcg cgcctggttg gtggagagga cggcgacgaa
      421 ctggttctcg gagagcttgc cttccctaac gccgagcgtc cagagggtca tggcgcgttc
      481 ttcggcgccc ggcagcccgt tggggatcag ggtgaagtcg tctttgccga gctctttctg
      541 ctcgcggtag ttgaacgagc agtgatccga gccgaagatc tggaggtcac cgaacttgag
      601 gccgttccac agcgctggtc tgttttccgg gtccctgagc ggcggggagc agacatactt
      661 ggcgccctcg aatccctcgc ggtcgagatc ctcatactcg aaggcaaagt actgggggca
      721 ggtctcggcg tagaccgtct ggccacgctc gtgcgccagg tggatggctt ccagcgccgg
      781 cgcgcacgag acgtggacga cgagtatcgg cgctccggcg acctcggcta gccgtatcgc
      841 cctgttcgtc gcctcggcct cgacgggctc ggggcgggtg agggcgtgga agcggggggc
      901 cgtatcgccg cgcgagaggg cctgttgctg gagcttggcg atggcatcgc cgttctcggc
      961 gtgtaccatg accagcaccc ctgcatcttt ggatagttga agcgcctcga agaggtcttc
     1021 gtcctccgtg tagaggggcg ttcccttgta ggccatgaag atcttgaccg aggccacgcc
     1081 cttctcggcg agatccggta gttcggcctt tatatcgtcg cgcaggtcgg ttatggctac
     1141 gtggaaactg tagtctataa ccgccttatc cctggccttc tccgtccagg cgtcgaatgc
     1201 cccggcgagg gcgccgcccg cttcctgcag ggcgaagtcg atgatcatgg tcgtaccgcc
     1261 agccgcggcc gcttctgtgc ccgtcgccca gtcgtccgcc gtgacggtcc ctccgaaggg
     1321 catgtccatg tgggtatgtg cgtcgataaa gcccggcatg accagcttgc ccgacgcgtc
     1381 tacggtctcg gcgccgtccg ggtcgaggtt cgcgcctacg gccactatct tctcgtcctc
     1441 gacgagcacg tcggcccggt aactgccctc ggccgagacg acggtcccgc cggagaaaag
     1501 aagtccgctc atagtgttct ccttccactt ggcagcggtg tgttggctaa ctctcgcaca
     1561 ccctgcgcct tcgcgtcaat cgtgaatcgg gacgatctcc aagggcgtca ggaagctaat
     1621 gccccactgt caaaatagcc cgctctgtat cccggcatcg catagtaagg agatttcacc
     1681 cggcgagtct cggccgaggg ctgtgcagtc ttgcgtggct ggctacgtcg ccggaaagcg
     1741 atccgcacgg aatggtgcga tgagcggatc tatttcgccg gtcaggatct cacacgccag
     1801 cagtcgccct atcagagggc cgagagtgac gccgctgtgc gtcacggcct cgtagtagcc
     1861 cgggatctca gagagcgcgc cgacgcaaga atgcccgtcc gccggaacag gccgcatacc
     1921 gaaccgcgcc tccacgacct cagcctctcc gagcgccgga agtacgagcc gcgcccgttc
     1981 cagaagctca gcgcagagcg gatcctcggt tccggcgaag tcatcggtga gcagctcgtc
     2041 gacggagtcg tgatgcagca agacgtatcc gtcaccgtcg ggacgaacgt tgatgcgcgg
     2101 tgtgtgcatg aggtgtctca gaggctcgtc aaggacggcg acccgcacga gcagtcccgg
     2161 gaatacgtcg agcggcaact ctctcccgac catttcagcc acggatgcgg ccttcgcacc
     2221 cgtagcgttg acgacagcat cggcatgaac ctctccatcc tccagtcgca gcgtaactcc
     2281 tttgccctcc acctcgatcc cgcgcaccgc atccccgaaa cgttcatgtg cgccattgcg
     2341 gacggcagct tccacgagtc ttctcgtcag agccggagca tcgacccagg attcttcagg
     2401 gaagtgtgcg atgcggatct ccggtaaagc tgctccaggt tcgagcttcc cgtttacggt
     2461 ggctgcgggg agcatctcgg cagcgtagga ccaggagcgc aggcgttcga cgcgttcttc
     2521 gaggtttgcc agctcatcgt ttgcggccca gatcaaattc cccgtggggt gaagccaacc
     2581 gtcaccgatc tcatcacgga gccgctcgtg ctcgcgcatc ccggccacgt ttagctcgaa
     2641 gtagtcgcgg gggagcttgt tgttggcgtt gacccaggcg aagctggtcg aagtcgtccc
     2701 ggaacccggt tccgctccgt cgatcatgac tacctcggcc ccaccctcgg agagacggta
     2761 ggcgaccgag ccaccgacga taccggcccc tatgatcgcg accctcaacg cgcctccacg
     2821 ccgggcagaa tcgtctcttt cattcatgct gcgtccaggt ccaaactcta cactcgctcc
     2881 cttcctgcgt acggccttcc tgggctgcat ccaaaacgcc cgtagaggca gaagaggtgc
     2941 gcagtccggg taccagcggt tatccggtgc cgatagccgg ggcggaaggg tcaggggcgg
     3001 gggatctcgc gttcaggtag ccgaggacga agaccgcgcc ccatagtagc acgaagccaa
     3061 ggacctggtt tgcgctcacg cccacgccga ggaacgccca gttgaggacg accgccgttg
     3121 ccgggaaggc caactccgcg agcgtcgcgt aggaggccct ggtgccggtg aggcccctgt
     3181 agtacaggag cagggccagg aggccgggga tcagggcgag gaggatcacc ctgcccggct
     3241 cagcagcgaa cccggtgccg agcccatcca aggaaccctg ggcgatcacg atgccggcga
     3301 gtagtggcac ggctatgagg agtcgcgcgc ctgtcagggt attgaagggc atgtctttca
     3361 agaccagccg tcccagcacg gtcgaggatc cccacaggag cgcagccccc agggcgagga
     3421 acgccgcgag agcttcggcg gagccaaggg ccatgaacgg actcaaatct ccgaaggaga
     3481 tcaggtacgc acccaccata gcaaccgcga agcacggcca gtaggcccac cccagcctct
     3541 cgcgcagcaa gatggccgcc agcgcgatgg cgaaaagcgg ctgggtcttc tgcaacagga
     3601 tcaccacggt cggattgccc acggcgaacg ccgtcgtaaa gagcagtgtc gccagggccg
     3661 aaccgcccca cccgatgatg agcagcgcga cccactgcgc cgttccgagc ccgcggaaga
     3721 acctccagcc caacaccacg gccggtaccg aatacagaag caagaccaga tgctcgagga
     3781 gcactatctg agacggtaag gccaccttca gcaagggcac ccgcaggatg ccgtccgtcc
     3841 cccacatcgc cgccccgacg gcgacgagca gtacgctagc gtagcctccg ttcctccaac
     3901 ccatgcgcta tgctttctcc ggccactcga gcttgggtat cacgcgctct ccgtaagccc
     3961 tgatgaactc ctcctggttg cgcccgacat tgtggatgta gacttcatcg aagccgaggt
     4021 ctacgtagtg ctggacgtgc tccaggtgtt cgtccaagtc ggagctcatc aggacccgat
     4081 tcttgaagtt ctctacgctt acctgcctgg ccatcgccgc gaagtcttca ggattcctta
     4141 tgtccgcctt ggggaagggc atgccgccgt tgggccactc cctgacggcc tgctgctcgg
     4201 cttcttcctg atcttcagcc caggagacgt gcagctgtat catgcgcggc atcccggagg
     4261 ggtctttgtc cgcgcggcgc gctccttcct cgaacttctc catcaggttc tttatcttct
     4321 cgtccggggc accgacggtt atgataccgt cgcagaactc tcccgtcctg cggctctgta
     4381 tagggcctgc cgtggccacg tagatcgggg gcggggtctc cggcatggtg tagagtctcg
     4441 cgctctcgag ccttatgtgc tcgcctgagt atttgacgac cttgcccgtg aagagcctgc
     4501 ggatcacctc gatggcctcc atgagtatgc gcagcctcgt cggtgcctcg ggccagtaat
     4561 cgccgacgat gtgttcgttc agcgcctcgc ccgcgccgag accgagccag aagcgtccgg
     4621 gatacatagc ttccagcgtc gccgccccct gggcgatgat cgccgggtgg taccggaagc
     4681 cgggcggcgt tacgcccgtg ccgaaccgga ggttcgtcgt cgcaccgagt gcgcccatcc
     4741 aggaccatac gaaggcgctc tggccctgat ccggggtcca agggtggaag tgatccgagg
     4801 ccattaccga accgaatccc gactcttcgg ccgtcttact ccaattcaga agatccgtgg
     4861 gatggaactg ctcgaacatc gccgcgtagc ctacggtacc cgtagtcttc atgtcgctcc
     4921 cttcggtcgc tcctagcatt tcagctggtc agcatttcag cacttcagct tcgctctggt
     4981 cggatcttcc gctgttgcgc caaacgctag tttacagcgg gaccaacggt tgtcgctctg
     5041 aacatgccgc tgtccgcgaa caagctgaaa tgctgaccag cggaggcgcc ggccggagcg
     5101 tgctgaccag ctgaaatgct aatcaaaaat gatccggccc atagagccac gggtctccca
     5161 gggggaaaat cgcctcgagg agatcgagag cttcgggaga gctcgcctgg atgagcccgt
     5221 gctttgcaag accgccggcc ggaaggtacc cggcgtagag ctgggcgagc tggcgcacgt
     5281 cgagagagac tctctccccc gcttccgtac cgcggattac cttgccttcc gcgagggtgt
     5341 actcgccgga gttctcaggt atgacgtcgt ccgatacgtc gagtacgagc ggcgcctgtg
     5401 gcgtgcgccc gaggtacccc agggcgactt cgacgtcgac gagacgcagc atctggtcgg
     5461 gttcgatctt cgcctctacg tgcgagcttt tcaggtaggg gtgaagcggc tctccgcgcg
     5521 gggtgtagtg ttttatgccg tagaccaggg gatctaggcc cgccataaag gagtagagtg
     5581 ccgccctcgc ccgctgtgtt gcggcgacga gctcctctac cgagagggtg cgccgcggat
     5641 cgcgctcttg ccacccggag gtcttgtaga ggatgtagcc ttccacgtca ccgtccctct
     5701 catagacggc gacgtccgca tctttcttgg acagagtcgt tttccagtgt ccttcgctcc
     5761 tgcgtgcgct gagggtatgc cccttcgcct cctctgtgta gagctccatc aatgaggaga
     5821 gatcttcttc ccggtaggcc ctgaggtgac tctgttccgg gctggtcggg aggtcggctg
     5881 gcttgagcct gtactcgatt tcctcggtga ctagctcgta tccgaagacc ctgtagaagg
     5941 cgtgggcgaa gggggacaag agcgacagag cgacgtcccg ttcgcgcatg tcacgaatca
     6001 cggcgcgcat caacttcccc gcgtagcccc gccgcctgta ggcgggatgg gccatgactg
     6061 ctgcgatgcc acccatcggt cgccactcgc cctccacata agactccagc gggagcaccg
     6121 tggtgctcgc cctgacctcc ccatcctcct cgatgacgtg gacctgctcg agatccagac
     6181 gagcgttctg ggccgggtcg aagttcccct cccaatccga gatgctaccc ccgaaggccg
     6241 ccaccccaag acgcgtcagc gcatccatgt ccccgtcacg gtacttgcgt agctccatca
     6301 tcctccggct ctctagagtg cgcctggatt ctaccgaaag agcgaggcag ggatcacccg
     6361 tcgttcctct ttcgtccctc gatagaatcg gccatccacc tgcaccactc ggcgtattct
     6421 ctctcgtagc tgataccgcg ccggagcgtg gcgtaggagg cgaactcggg actatcggta
     6481 gaggtcaggt cctcccgcca ctcttcctcc atccaggttc ttagcccctc gtagtgcgcg
     6541 agctgctcct cgtgccttag ctcctcctca cggaagagcc gtatggcctt ctcttcgtca
     6601 gccagccaga ccgagtacgt cttcaacgtc aactcgtccc gcgtcggcct cggcacgggc
     6661 ggccgggtca cccactcccg cagcgcgtcc aatccctcgg ccgtgatctc atacaccttc
     6721 ttatcaggcc tctctttctg ctcgaccacg ttgtgcgcca cgtatccgcc atcctccaac
     6781 ttcgccaact cggggtagat ctggctgtgc ctcgcactcc agaagtatcc gacccgccca
     6841 gccatccgcc gcgtcagatc gtacccgctg agatcctccc tagatagcag accaaggatc
     6901 gcatacccta gcgtcgtcat actcctccaa tctcttgtta tgtcacactt gacatatcat
     6961 ccaccggcct ttagagtatc atatgtcaag actgacataa agaacgtgag agaggagcga
     7021 gagatgtgcg gctggtctta cgtgggacat ggtttaaggc ggggtgcggg tggtcgtgag
     7081 tttttggggt acgaggtgga gaagatgagt cctggggcga ggatctcggc cgcgctctcg
     7141 gtgcttgtgc cggtcgggct ctccggggta tttctggtcg ctttcgtgcc ttctctgtgg
     7201 tggatcttca cgacttattt ctggatcgct ttccctgctt tagggctgct cacgagtggc
     7261 ttaacggcac ttggcgaagg taggcctgtc cgggtctccg aagaggagcg ggagcgggag
     7321 ttgttggagg cgatccggga gagtggtggg atctcggccg ctgcggctgc ggcgaggacc
     7381 tcgttgacgg tcgccgaggc cgatgggatg ctaagggcat tggccgagaa cggccacctc
     7441 gaggtcattg ccaggggtgg agcgatcttc tacgcgttgt ggggaaacga gtgggagacc
     7501 gcacggagga tcgagggttc ggcgtgagcg cggtgtattg ttggatcaga tccctttcga
     7561 ctcggacagg agagtaggat ggcgcaggta gtagacggca ggtacacggc gcggatggac
     7621 gagccgttcg cggtattcat catcgggatg aggatcaaca acctcttcgc ggtaaggaag
     7681 tggctcccga ccatccgtgc gatgggcccg atgctaaagg agctctacca gcaccccgag
     7741 aaaggcttcc tcggcggcga gttcttcctc tactggcgcg ggcccgcgct cgtccagtac
     7801 tggcgttcct tcgaggacct ggagcgcttc gccaggaacc cggacgatcc gcacctgcct
     7861 gcgtggcagc gcttcaaccg gacggtaggc tcggacggca gcgtgggcat ctttcacgag
     7921 acgtacatag tggagcgcgg gaactacgag gcgatctacg gcaacatgcc ggtgttcgga
     7981 ttggcaaagg cgaccgagca cgtcagggcg gtcggaggca gggagaccgc gcggcggcgt
     8041 ctgatgcgcg gagagaacga accggccgtc ccttcgcctg agtaacgagg gtgagccatc
     8101 tagactacct cctcttgccg cccgagcggc gcagaatgga ctatgaactc ctcaatgacc
     8161 tacggcgacg tgcataaagt ttggcctctc atgaacgacg ggctatcagc gctcggctcc
     8221 catcgctcag cccttgtcct ggctgatagc tgatggctcc attctcggaa aggttcatcg
     8281 ttttcgagga tggctctagg ctcgccagaa tgccgggcta agcaggacca gaaccgtgaa
     8341 gagttccaat cgtcccagga gcatcaagat ggcgagagtg ctcttggcgg ttgcgggcag
     8401 atcggcgtag aactgtgagg tgcctgctgc gcctgggaac gtgcccgcta tgttcacgca
     8461 cgagaagacc gatccaaaag cctgccccat gggaacctgg tgcagggcga tgagaagagt
     8521 gccaacggcc acggttgcca cgtacacgaa gaagaagctg aggaccccca cccgaatccg
     8581 ctccggcagc acccggtcgc ccaacaccag gggcgtgacg gcccgcgggt gaaccaggcg
     8641 aaacatctcc tgggcggcgt gacgcgtcag aaggtaggcc cggatagctt tcatcccccc
     8701 ggctgtcgag cccgcgcaac cgcctacggc catgatcaac acgagcagcg ctacggagaa
     8761 tgggggccag acgctccagt ccgttgtgct gaagccagcc cccgtggtta gacttgcgct
     8821 ctggaagagg gcctcgcgga aggctatagc caatgagttc tcgtagtcgg agactcgaag
     8881 atcggcagtt atcaacaaag tgctcgcgaa tattaccccg aggtaggtga cgagctcggc
     8941 gttgccgacg gcacgtccca atccacgccg ggccgctatg tagtagatgg cgaagttcgt
     9001 gccggccaac accatccccg ccgtgatcgc cagctcgacg gcccaggagt cgaaggctgc
     9061 cacggagtcg gagtcggtag agaacccgcc ggtcgagacc gtgctgagcg cgtagttgac
     9121 cgcgtcgaat gttgccattc cgaccaggtg caggacgacg acacccccca gagtaaggcc
     9181 gccgtagacg aaagccagca ccctgaccgt gtcccgaatc cgaggggtaa tccgctcctg
     9241 cactggggtc gccgcctcgg ctgtgtagag ttgggtggcg ccgaaccccg ccaaaggtgc
     9301 gatggctacg aacagcacga tgatgcctag acccccgatc cactgactga ggctgcgcca
     9361 gagcaacaag gcgggtgaga gctcttccgg agtgaccgtt gcggccccgg tggtcgtgaa
     9421 gccggccatg gagttgaaga aggcgtccac cggacccatg accccggcga ggagaaacgg
     9481 cgcagctccg actaacacca cgcccaacca gcacacgacc accatgagga agatgttctg
     9541 tctcgatacg tacctgttgt gcctggaccg tgtgagaaag aagagggcca gcccaaggac
     9601 cagggccgtc gttgctggta tccagaagga ccagccctcg ttagggcctg tcagcatcga
     9661 gtaagctccc gggacgagca ttacggctcc cacggcggca acgagcgccg ccagcgcatt
     9721 cgcgatcacc ctcaggttca gagcgctcac actatccgcc cacgctggtt agaaggatcc
     9781 tgtcgcgacc atcctcatac cgtgctcact ctaccatcgc gcttcgtccc gattacgcca
     9841 acggtgtcag cgccggatgc tggggcctat gcgctttgcg gcggtgcgga agtcgtaggt
     9901 ggtgaccgag agggccgaga gctggtggga ggctatccgc ttctcgaagt cgcggaggtt
     9961 tatggtacgg cgcatgcata cgagggcggc ctcgcggcag agggcttcca ggtcggcgcc
    10021 cgagtagctc ccggtgagtc ccgctatctg atccaggtcg aggtcggggt ccgtgggcat
    10081 ctcgcgggtg tggatcccta gtatgtgccg ccgcgcttcg gcgtcgggga gcccgatctc
    10141 gatctcatgg tcgaagcggc cagggcgcct gagggcgggg tcgagcgcgc ccggcctgtt
    10201 ggtggtggct atgacgcaga ccctgcccag attattgagt ccatccatga gggagaggag
    10261 ttgggagacg agggtagcct cgaagctctc gctcatcatg tcgcgcgacg aggcgaagga
    10321 gtctatctcg tcgaagagga tgatcgccgg cgctttggcg cgggcttcgt tgaagatgga
    10381 gcgcagcttc gcctcgctct ggccccagaa cttgttgagg atctcgggcc ccgagacggc
    10441 cacgaagtgg gccccgctct cgtgggcgac ggccctcgcc agtagcgtct tgcccgtccc
    10501 cggtggcccg tagaagagga tgcccttgtg cggcctgatg tggagcttgc ggaagatctc
    10561 ggggtgggtt atgggcagtt cgacggactc gcggaccagg tcgatggtct cgcgcatccc
    10621 gccgacgtcc tcgtagcggg tagcaggcac gttgcggctg ccgggcgccg cgccgctcgc
    10681 cgccgcgacc cccgctcccc cactccgcac gctccgcgcc aggcgttcga tgaagccctc
    10741 cctgtcctcc tgcagatcag cctcttcgcg cccttcccgc gtcttcacga ccctccagcc
    10801 cgacaggtct ccgacttccc tgtaacccca ggagttgtcc ctgctagggt ctatcctctc
    10861 cgggaggcgg gccactatct cggcggtcga gtcgaggtcc aggatggggc acctgacgca
    10921 cgggccgaga tccgagagga cacgctcggt gatggcgtcc ttgtccacct cgaagactgc
    10981 ctcttcgtcc atcttgccga aggcgtacca cgagttggcc gtgagatggt cgttgtcgta
    11041 gacccggatc tgacggcaag ggaagagctg cttgtccgcg agggaaatgg gcgtgcacca
    11101 ctccctcgcc gtcctcccgg agcgttgctt gcgcaggaaa cacctcaagc ccccgtagag
    11161 ctcgtccgcc gagagcgagg ctccatctgc cgcgtactcg gtgtcgacct cgccgcgcag
    11221 gagccccgag aagtcgtcga ggagccgcca gtcggaaggg agatccaggt ccacacagac
    11281 ccgggccgag ccgttcacgc cctctacgcg gtaggaagga tggcgggacg caagcgccac
    11341 ggcccggccg agatggcctt cggatctcaa cgggaaaccg actctcactc gcatggccag
    11401 cagattctaa cccacgccac ccgcggacgg aggaatccgt acattcctcc ccctcggctt
    11461 gcgctacgcg accgggcggg ctaccgtgcc cgtgcatccc gaagagaaca ggagaccctt
    11521 tgagtgagca agatagcaac ccgccggtct cgacgggcgg gggtgacgac gggacgacga
    11581 ccctgctcgg gtcgggacgg atgggcaaga acgatgcacg gatcgtcgcg ctcggcgaca
    11641 tcgacgaggc gagctctttt cttggcctgg cccgcgccga ggctgacgag gaggtcggtg
    11701 ggctcttgat ccggctgcag cggctcctgt accgcgtcat gggcgacctg gcgatgccca
    11761 aggaggacaa cgtagtcgac gaggatgacc tgaagcacct cgatgacgcc ctcgccgagt
    11821 ggcgcggacg cacccggatc cccagggaat tcgtcattcc gggtgagagc cggcgcggcg
    11881 cgctactcga cgtcgcccgc tccgtagcgc gccgcgcgga gcgtagcctc gtcgccgccg
    11941 gatacgcgaa cgagcacccc tacgccgtgc gcgtcgtcaa ccgcctctcc gacctcctct
    12001 tcgtcctcgc ccgcaacgcc gacggcgaga agaccctctc taaggggtag gggcgggttt
    12061 gaaacccgtc cctacctgga cggcgactaa taggctcctt tgtcgacaaa gagagctctg
    12121 aaggtgcggg ccaaaagcac cagatccagc cataccgacc agtcgcggac gtagtggatg
    12181 tccatcccga tcctgtcagt aaagaaagcg tggttgcgac cggaaacctg ccacggtccg
    12241 gttacccctg ggggtacgcg cagaatctcc tcgcgcgcgt ccccgatatc tcccgactcg
    12301 cgaggcaggt agggacgagg gccgaccaag ctcatctccc cgcgcaagac gttccagagc
    12361 tgcggcagct catccaggct ggtcctgcgc aagaatcggc cgacgcgcgt gacgcgggga
    12421 tcgacgcgca gcttgtggta ctttacgtac tcttccctca ggccggcgtc ctcggcgagc
    12481 ataagctcca gcacggcctc cgctcctggt accatggtac ggaatttgat gcacgggaat
    12541 gggacaccgt ccttacccat ccgccagtcc gtgtagaaga tgggccttcc ggactctaca
    12601 tagacgagca gggctatcac caggagaagc ggaagcacga gggctccgcc caacacggta
    12661 gctcccaggt ccagtacgcg tttcgccatc cgcgcccagg ggctgaggag gttgtacttt
    12721 atctccaccg ccagggttcc cgcaaagtcg cgggccgtta ccgccgagtt
//
LOCUS       DATMVF010000029        11041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_187963, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000029 DATMVF010000000
VERSION     DATMVF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187963"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..837
                     /locus_tag="VN178_01120"
     CDS             433..837
                     /locus_tag="VN178_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79592.1"
                     /translation="MRRLAGRHAVIRLIVVWVVTAVTLFVLSSLIPEIDIPGWGSAFA
                     IAALPSLGPGHLDPRRAAETLRLLHPRLAVPIHWGTYAPLGFGRLQTALLADPVTEFR
                     RHAAELAPEVEVRVLDLGGTLHLDAVAGGDQG"
     gene            complement(1025..2020)
                     /locus_tag="VN178_01125"
     CDS             complement(1025..2020)
                     /locus_tag="VN178_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HWS79593.1"
                     /translation="MQESERVDTEMVMNGRKHMRDAVFFEGPEVRQRSSRFWILITLA
                     SIIAAAGVAADSTATVIGAMIIAPLMIPILGTMLSVVLADRTNLIRSLLHVAGGAVIA
                     ISIGWLVGVLVVNEVVAETNSQVAQRVHPKLIDLLAALATGVVGSVALVRRDISDTLP
                     GVAIAISLVPPLCVVGFTLEAGKLDQSLGASLLFMTNVAAILGSGTVVMALYSFHRLV
                     AGSEGRTFNRRPAVLAIAALIIAVSVPLTATSITASRDTAREAQTLAAARAFGNEVGW
                     TTGNVNTRGGVVIVHMEGPPPLPRTDRLQKELEERGVDPSDVQVELVPARRVTFD"
     gene            complement(2157..3563)
                     /locus_tag="VN178_01130"
     CDS             complement(2157..3563)
                     /locus_tag="VN178_01130"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HWS79594.1"
                     /translation="MKVEQGPNLSPAAAQAAASYQERHERGCAARRAAPRGGHAEWAP
                     APDRPDPIDLLEAQDRNRIPDLIPIRYSRMMASPFAFMRGAAIVMAHDLAGTPKTGIR
                     AQLCGDAHLSNFGAYASPERALLFDLNDFDETLPGPWEWDVKRLAASFVVAGRENGFD
                     AAECREAAQASAASYRRRMSEFSKMGELEVWYSRVGVEEVRGLLSDARARKATAKKLS
                     KSVRKARGRDSLQALSKLTRVVDGHRKIIDDPPLLVRIPEGDEIRSQIYAILESYKRT
                     LQEDRRYLLDRYRFVDVARKVVGVGSVGTRAYVALLEGHDENDPLFLQVKEAGPSVLQ
                     GPLLSSTHEHHGHRVVAGQRLMQAASDIFLGWFRGKEGRDFYWRQLKDMKGSANVEGM
                     SPDELVIYAGLCGWALARAHARSGDRVQIAAYLGKSERFDGAIADFAEAYADQTEQDH
                     AVLCAAVESGRVPTDESV"
     gene            complement(3646..3864)
                     /locus_tag="VN178_01135"
     CDS             complement(3646..3864)
                     /locus_tag="VN178_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79595.1"
                     /translation="MEQNEDPRPAIGEGLAAPPTEASLRMQVTDLLLGRSHDVLVGRS
                     TREQGDVLRHIEAALETYGSYFYPPEHR"
     gene            complement(3880..5361)
                     /locus_tag="VN178_01140"
     CDS             complement(3880..5361)
                     /locus_tag="VN178_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWS79596.1"
                     /translation="MSQEGEVRHEDLPIPEYVASRGARLEGILGRVSDAPLREGNRLE
                     LLRNGPDTYDDWLRAISRAERWIHLDNYIFANDEIGNTFAEALSARAAEGVRVRVLHD
                     WFGCMDVPRSFWNGMRRSGVQVKAVNPPASGPPLGAIRRDHRKLIVVDGEYASTGGVC
                     IADGWMVRSKETGLPYRDTAVSVGGPVVADVNRAFTKLWGELVEELPDDEHPDPEGIP
                     AAGETPARLVVQEPRRMRTLRMLEFLTAGVQERLWVTDPYFLSLPILTQSLMATARDG
                     VDVRVLTPATNDIAWIGRASRAGYRQFLEAGVRIFEYGGPMIHAKTIVADGWWSKVGS
                     TNLNFSSLVANWEVDLVVEDTDFAKGMEQLFEDDVSNSREVRLEGSGQHRRVRPAGQV
                     DTRDSGARAGVVGSGTGSGATISRVVPTVVQKGAAPLQTHEHAIAAAASGVLLGASLL
                     GARFPRLVAWPLTAVGAFLGGLGVLRVVRSRLSDREPDRSGAT"
     gene            complement(5408..6037)
                     /locus_tag="VN178_01145"
     CDS             complement(5408..6037)
                     /locus_tag="VN178_01145"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HWS79597.1"
                     /translation="MSLAVLPLAITMNAGPQIMSAIIFVTAPKALKLSAYFLAGVVIA
                     VTVGVTITYTLATVLGNSVSLGDSSDSGSLGNIIQYLFVGLLVFLSIKNFVNRETIEP
                     PRWLGAMQNAKPRTAFMAGLLLLSVFPSDFVVLMTVGINLAQQNSSLLAAAPFVLATI
                     LIAALPVLSYLLFHRRAVRVMPKVRDWMNANSWLVNIVVYIIFIILILA"
     gene            complement(6034..6303)
                     /locus_tag="VN178_01150"
     CDS             complement(6034..6303)
                     /locus_tag="VN178_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79598.1"
                     /translation="MMGKGTGTVYRIVVRSELNNRYAVAFEGMEMETRDGNTVLTGKI
                     IDQPHLHGILARINGLGLRLLSVEALPEDVDPSPVITREPKGSEL"
     gene            complement(6430..8649)
                     /locus_tag="VN178_01155"
     CDS             complement(6430..8649)
                     /locus_tag="VN178_01155"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS79599.1"
                     /translation="MIVLKPKLRAPVPRSEQLVRRRMFDLLQNATDRKLSIISAPTGY
                     GKTTLLAHWRRVEVANLSFAWVSLDEQDNDPIRLWRHIVEALHRAVPDGEDFGTDVLV
                     GMSAVGQGFVATTLPSLINELAELSHRVVLVLDDYQFVTEEDAHESVAFFVEHVPDNV
                     HLVISSRTDPPLPIGRLRARGEMNEIRTEQLAFSEEEAECLLNEKMGLDIGPEDLSVL
                     LERTEGWPAGIYLASLSLQNKEDKHAFIASFRGSNRYIVGLLGEEVLADLPEEVRQFL
                     LETSVLRAMTGPLCDAVTGRQGSASLLRELTRSNLFVVPLDEQGEWYRYHHLFSELLL
                     FELKSSRPNLVPTLRKRASVWLEGVGSFEGAIRQAIEAADYERVGLLIARYWYGYVSA
                     GQTATVQRWLESLPEEMITHNAALALVKAWICALVGQREETARFLALAENLPYEGPLP
                     DGTTSVESGMAILRAVFGYDGVQSTVEAVRLAIELEPGESSPWAALVRFALGTSLFLS
                     GDTSRARRPLEEALVLTENGQRLVRMVTLSFLSFVAADEGHLEEAESHARAAQALVEG
                     LRPFAIPQTSLAPIALGRALAERGNLVEAQAELESALSARRRIPGLSPWPTLIGLLAL
                     ARVRAARGDRAGGRATLAEARAILEAFPDSGVFPDLLERQERKLRAHKRQLDGELTER
                     EMDVLRLLGGELSTRQMAQSLYVAPSTVRTQIKSIYRKLGVSSRSAAVEDARARGLI"
     gene            9307..9759
                     /locus_tag="VN178_01160"
     CDS             9307..9759
                     /locus_tag="VN178_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79600.1"
                     /translation="MTQHSEPVPGGVGSPMESPAGASRVEHRPGMLTFAAVVMFMVAG
                     FEALSALLAFAGSGWWVTETGNLVYANFVLWGIVDLIIALIALYAGIDLLRGGEFGRA
                     MAYVFAIVGAIRGLFVIPAAPVLAVVVIALCVMVIYALAKHSDYSQSA"
     gene            10012..10503
                     /locus_tag="VN178_01165"
     CDS             10012..10503
                     /locus_tag="VN178_01165"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HWS79601.1"
                     /translation="MATLSVLKFNDPYGAERVMVALQGMQERQMITLEDAAVVSWPQG
                     NKKPTTRQLHSMAGAGAGWGAFWGFLFGLIFFVPFLGAAIGAGMGGLSGAMADVGIDD
                     DFIKEVREKVTEGTSALFALTSGATAPDKVIDELKQYDFEIISTNLPKEQESKLREAF
                     AQE"
     gene            10712..>11041
                     /locus_tag="VN178_01170"
     CDS             10712..>11041
                     /locus_tag="VN178_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWS79602.1"
                     /translation="MTDLLVLAAVIFVFGLVSRRLEGTVLTAPIFFVVAGALLGPAVL
                     GLVEFGLDEHTVLLLGEIALAIVLFTDAAGIDFSALQQYQGLPLRLLGVGMPLTIALG
                     TTTAALLL"
BASE COUNT         2028 a         3475 c         3422 g         2116 t
ORIGIN      
        1 gtttgaccga agagcggctc actgcgaagg tcttggcggc ctcgctctta ggcatccctc
       61 tgtcgacggc ttctaggatc ttggtgcgca gatcttccga ataggctttc atgcaccaat
      121 gatgtacccg gctcaatcac agcgcaacct gctctaggaa ccaccacgaa cccgaaaatc
      181 ccctggggga tttccggctg cgaatgatga ccaatcacca tgctgggcct ctctctagag
      241 aggcgttatg caaagtggcg gccagaagat cggatctgct cgaaaggacg ttacgatgga
      301 tcgcaacccc gacaggcgct cccttctgtg gatgctccct ggtgatcggg gaaccaatcc
      361 gcccgatcat gcagaagaac gcacgagcgg cacccacact ttggaccgga ccgtgaccac
      421 ccgaggccgt tcgtgaggcg cttggcaggt cggcacgccg tgatccggtt gatcgttgtg
      481 tgggtggtca ctgccgtaac gctcttcgtc ctctccagtc tcatccccga gatagacatc
      541 ccgggctggg gctctgcctt cgctatagcg gcactgcctt cgctcggtcc gggccacctc
      601 gatccacgcc gcgccgccga gacgctccgg ctcctccatc cgcgccttgc ggtgccgata
      661 cactggggaa cctacgcgcc cctcggcttc ggacgtctgc agactgcatt gctcgccgac
      721 cccgtaacgg agtttcgccg ccacgctgcc gagctcgcgc ccgaggttga ggtgcgtgtt
      781 ctggatctgg gcgggactct ccatctcgat gcggtcgccg gtggcgatca gggctgaacc
      841 gagcaagaag tggcctgctt tcgctgcacc gaagccgact aaagaatagt aggcgaaagc
      901 cttggagagg gttggttctc gctgtatgcc actgctcttc cagcagggag acgatcggtc
      961 cgtgccgtct acaggagtgc tggtggccca ttgggaggct gcttcagact gtgggacgat
     1021 ccgctcaatc gaaggtgacg cgccgcgcag ggacgagttc gacctgtaca tccgagggat
     1081 cgacccctcg ctcctcaagc tccttctgca gccggtctgt cctgggcaac ggaggcggac
     1141 cctccatgtg gacgatgacc acgccgccgc gggtattgac gttgcccgtg gtccaaccga
     1201 cctcattacc gaacgcgcgt gccgccgcca gcgtctgggc ttcgcgtgcg gtgtcgcggc
     1261 tggcggtgat ggatgtcgcg gtcaagggga cactgacggc aattatcaag gcggcgatgg
     1321 caagtaccgc gggacggcgg ttgaaggtcc gcccttcgga tccggccacc aggcggtgga
     1381 aactatagag agccattact accgtaccgc tccctaggat cgcggcgacg ttcgtcatga
     1441 agaggaggga agctcctagt gattggtcta gcttaccggc ttcgagggtg aagccgacca
     1501 cacagagcgg gggcacgagt gagatggcga tggcgacacc aggcaaggtg tcggagatgt
     1561 cgcgtcgcac tagggcgact gagcccacca cgccggtggc gagcgccgcg agcagatcta
     1621 tgagcttcgg gtggacgcgc tgggcgacct gcgagttcgt ctcggcgacg acttcgttga
     1681 cgacgagtac tccgaccagc catccgatgg agatcgcgat gacggcgccg cccgccacgt
     1741 ggagaagcga gcggatcagg ttcgttcgat ctgcgagcac gaccgaaagc atggtgccga
     1801 ggatcggaat catcagggga gcgatgatca tggctccgat cacggttgcc gtggagtcgg
     1861 cagcgacgcc ggccgcggca atgatggatg ccagcgtgat caggatccag aacctactcg
     1921 accgctgacg tacctctggt ccctcgaaga acacggcgtc gcgcatgtgc ttcctgccgt
     1981 tcatcaccat ctccgtgtcg actctttctg attcctgcat gctcctaccg ttcgctacgt
     2041 tttgcccgga tcagtcgccg gtcaacgttg taccaatgcg cgtcagcctg gctcgcgagg
     2101 aggaaatgag gctggatgtc gtcggacgac cggtttggtc tacccggacc ggaacctcag
     2161 acactctcat ccgtcgggac cctaccagac tcgacggccg cgcagaggac cgcatggtcc
     2221 tgttcggtct gatcggcgta ggcttccgcg aagtcggcta tggccccgtc gaagcgctcg
     2281 ctcttcccca ggtaggcggc gatctgtaca cggtcgccgg agcgagcgtg cgcccgggcc
     2341 agcgcccagc cgcagaggcc ggcatagatg accagttcat cgggcgacat gccctcgaca
     2401 ttcgccgaac ctttcatatc ctttaattgc cgccagtaga agtcacgtcc ttccttgccg
     2461 cgaaaccacc ccaggaagat gtcactcgcg gcctgcatca gtcgctgccc ggcgacgacg
     2521 cgatggccgt ggtgctcgtg ggtgctcgac agaagggggc cctgcagcac cgagggtccc
     2581 gcctccttga cctggaggaa cagagggtcg ttttcgtcgt gaccctcgag caatgccacg
     2641 taggcccggg tgcctacgct cccaaccccc acgaccttcc tagccacgtc cacgaagcgg
     2701 taccggtcga gcaggtagcg gcgatcttct tgcaaggtgc gcttgtaact ctccagaatg
     2761 gcgtatatct ggctcctgat ctcgtcccct tcggggatac ggaccaggag cggcgggtcg
     2821 tcgattatct tccggtgccc atcgacgacc ctggtaagct tggagagagc ctgcaggctg
     2881 tcgcggccgc gcgccttccg cacggacttg ctcagttttt tggcggtcgc tttcctggcc
     2941 ctggcatcgg agagcaaacc ccggacttcc tccacgccga cgcgcgagta ccagacctcg
     3001 agctcgccca tcttcgagaa ctcggacatc cgccgccggt aggaggccgc agacgcttgc
     3061 gcggcctcgc ggcactccgc cgcgtcgaag ccgttttctc ggccggccac tacgaagctt
     3121 gcggcgaggc gtttgacgtc ccactcccag ggcccaggca gggtctcatc gaagtcgttg
     3181 aggtcgaaga gcagggcgcg ctccggggag gcgtacgccc caaagttcga gaggtgggcg
     3241 tcgccgcaca gctgggcccg tatcccggtc ttcggcgtgc ccgcaaggtc gtgggccatg
     3301 acgatggccg ctccccgcat gaacgcgaac ggcgaagcca tcatgcgcga gtaccggatc
     3361 gggatgaggt ccgggattcg gttcctgtcc tgcgcctcca ggaggtcgat cgggtccggc
     3421 cgatctggtg ccggcgccca ctccgcgtgg ccgccccttg gggccgcccg acgcgcggcg
     3481 cacccacgtt cgtgacgttc ctggtagctc gcggccgcct gcgccgccgc aggcgagagg
     3541 ttcgggccct gctcgacctt catggattcc tccttctcag tcctgaaagc acaagcactc
     3601 cgggtatacg ttcctcctcc ggcgtgcggt acggtgacct tctagttagc ggtgctcggg
     3661 cgggtagaag tagctcccgt aggtctccag cgctgcttcg atatgtcgca ggacgtcgcc
     3721 ctgctctcgc gtcgaccggc ccactagtac atcgtgcgat cgccccaaga gcaggtcggt
     3781 tacctgcatc cgcagcgatg cttcggtcgg cggagccgcc aggccctccc cgatcgccgg
     3841 ccttggatct tcgttttgtt ccataccctc tatccctcgc tatgtcgccc ccgaccggtc
     3901 cggctcgcgg tcggagagcc tggatcggac cacgcgcagg acgcccaggc ccccgagaaa
     3961 ggcgccaacc gctgtcaacg gccaggcgac caggcgcggg aaacgggcgc cgagcaacga
     4021 agccccgagc aggacgccgc tagccgccgc ggcgatggcg tgctcatgcg tctgcagagg
     4081 agcagcgccc ttctggacga ctgtgggcac taccctcgag atggtggctc ccgaccctgt
     4141 gccgctgccg acgacgccgg cgcgcgcgcc actatctctg gtgtcaacct gcccagcggg
     4201 tcgcaccctc cggtgttgcc ccgatccctc caggcggacc tcccggctgt tggagacatc
     4261 gtcctcgaag agctgctcca tgccctttgc gaaatcagta tcctccacca ccagatctac
     4321 ctcccagttg gcgaccaggc tgctgaagtt caggttcgtc gagccgacct tcgaccacca
     4381 gccgtctgcc acgatcgtct tggcgtggat cattgggccg ccgtactcga agatgcggac
     4441 ccccgcctcc aggaactgcc tgtagcccgc ccgcgaggcc ctgcctatcc aggctatgtc
     4501 gttggtggcc ggcgtgagga ccctcacgtc gacgccgtcg cgcgccgtgg ccatcagcga
     4561 ctgggtgagg atcggtaggg agagaaagta cgggtcggtt acccagagac gctcctgcac
     4621 cccggccgtg agaaactcca gcatcctgag cgtgcgcatc ctgcgcggtt cctggacgac
     4681 aaggcgcgcg ggcgtctctc ccgccgccgg aatgccctcc gggtccgggt gctcgtcgtc
     4741 gggtaactcc tccaccaact cgccccacag cttggtgaag gcgcggttga cgtcggccac
     4801 gacgggaccc cccacgctga cggcggtatc ccggtagggc agtccggtct ccttggagcg
     4861 gaccatccac ccgtcggcga tgcacacccc acccgtggaa gcgtactcgc cgtccacgac
     4921 gatcagcttg cggtggtcgc gccggatagc gcccagcggc ggaccagagg cgggcgggtt
     4981 tactgccttg acctgcacgc ccgatctccg catgccgttc cagaaggagc ggggcacgtc
     5041 catgcaaccg aaccagtcgt gtagcacgcg cacgcgcacg ccttctgcag ccctggcgga
     5101 aagggcctcg gcgaaggtgt ttccgatctc gtcgttcgcg aagatgtagt tgtcgaggtg
     5161 gatccagcgc tctgctcgtg agatcgccct cagccagtcg tcgtaggtgt cagggccgtt
     5221 ccttagcagc tcgagccggt ttccctcgcg cagcggcgcg tcgctgaccc gtcccaggat
     5281 cccttccaaa cgggcgccgc gcgacgccac gtactccgga atgggaaggt cttcgtgtcg
     5341 tacctctccc tcttgcgaca tgatggttcc tccttgggtt tcattgccat gcttgcggac
     5401 ctcgacgtca ggcgaggatt agtatgatga atatgatgta gacgacgatg ttcaccagcc
     5461 agctgttcgc gttcatccag tcgcgcactt tcggcatgac ccgcactgcg cgcctgtgaa
     5521 acagcaggta agagagcacc ggaagcgccg ctataagtat ggttgccaag acgaacggtg
     5581 cggcggctag aagggaggaa ttttgctgcg cgaggtttat ccccaccgtc ataagaacga
     5641 cgaagtcgga agggaacacc gagagcagta gcagcccggc catgaacgcc gtcctgggct
     5701 tggcgttttg catggcgccc agccacctgg gaggttcgat agtctcgcgg ttcacgaagt
     5761 tcttgatgga caggaatacg agcagtccga cgaacaggta ctggattatg ttgccgagcg
     5821 atccggaatc tgaggagtcg ccgagcgaga cgctgttgcc gagcacggtg gcgagggtgt
     5881 aggtgatcgt aacgcccacg gtgacggcga tcaccacgcc cgccaggaaa taggccgata
     5941 gcttcagcgc cttgggggcg gtgacgaaga tgatagccga catgatctgt ggtcccgcgt
     6001 tcatcgtgat ggcaagaggg aggacggcca ggctcatagc tctgatccct tcggctctcg
     6061 agttatcacg gggctcgggt cgacatcttc tggcaaggcc tccacgctca agagtcgtag
     6121 ccccaacccg tttatgcgag cgaggatacc gtggaggtgt ggctggtcga tgatcttccc
     6181 cgtaaggacc gtatttccgt ctctggtctc catctccatc ccttcgaagg ctacagcgta
     6241 cctgttgttc agctcactcc gcaccactat gcgatagacc gttccggtac ccttgcccat
     6301 catctatcta ccgccgcact ccatcatgtt ctctgtcagc agcatcgttg ttcatcgtgc
     6361 ctgagtgagc gtagtgcacg gcaggatggt tcgtcttcac ccatcagccc atttccccta
     6421 ggggatttgc tagatgagtc ccctggcgcg agcatcttca acggccgcac tgcgcgaaga
     6481 gaccccgagc ttgcggtaga tggatttgat ctgtgttctt acggtgctgg gtgcgacgta
     6541 gaggctctgc gccatctgcc gggtagataa ctcgccgccg agcaagcgaa gcacatccat
     6601 ctcgcgctcc gtgagctccc catcgagctg ccgcttgtgc gcgcgaagct tccgttcctg
     6661 gcgctcgagc agatcgggga atacaccgga gtcaggaaaa gcctccagga tagctcgcgc
     6721 ctcggcgagg gtcgccctac ctccggctcg gtcgccacgc gcagcccgta cccgtgcgag
     6781 cgccaacaac ccgatcaggg tcggccaggg gctcaagccg ggaatccttc gtcgcgcaga
     6841 tagtgcgctc tccaactccg cctgggcttc cacaagattc ccacgttctg cgagtgcgcg
     6901 accgagtgcg atgggcgcca aactcgtctg aggtatcgca aatggtcgta acccttccac
     6961 cagcgcttgc gccgcacgcg catgtgactc ggcttcctcc aggtgccctt cgtccgccgc
     7021 aacgaacgac aagaaggata aggtgaccat ccgcaccagg cgctggccat tctccgtcaa
     7081 tacgagagct tcctcgagcg gtcttcgggc ccgggatgtg tctccagaaa ggaacaggct
     7141 ggttcccagc gcgaagcgca ccaacgcggc ccacggagag ctttcgccgg gctccagctc
     7201 tatggcgaga cggaccgcct cgaccgtact ctgcacgcca tcgtagccga atacggcccg
     7261 aagtatggcc atgcccgact ctaccgaggt ggttccgtcg ggaagaggcc cttcataagg
     7321 gaggttctcg gcgagcgcca gaaatctcgc ggtctcctct cgctggccaa cgagggcgca
     7381 gatccacgcc ttcaccaggg cgagcgccgc gttatgggtg atcatctctt cgggtaacga
     7441 ctccagccac cgttgcaccg tcgccgtctg gccagcggac acgtatccat accaatagcg
     7501 cgcgataagc agtcccacac gctcatagtc agcggcttcg atggcctgcc ggatcgcccc
     7561 ctcgaaggag cccacaccct ctaaccacac gctcgcccgt ttgcgcaggg taggcaccag
     7621 gttgggccgg ctactcttga gctcgaagag cagcaactcg gagaagagat ggtgatagcg
     7681 gtaccactca ccctgctcgt ccagtgggac cacaaacagg ttggagcggg tgagctcgcg
     7741 caagagcgag gcagaaccct gtctgcccgt gaccgcgtcg caaagtggac cggtcatcgc
     7801 cctcaaaacc gaggtctcca gcagaaactg cctcacttcc tcgggaaggt cagccagaac
     7861 ctcctctccc aagaggccga cgatgtagcg gttggagccc ctgaacgacg cgatgaaggc
     7921 gtgcttgtcc tccttgttct gcaaggagag ggaggcgagg tagatccccg cgggccaccc
     7981 ttcggtacgc tcgagtagta cagagaggtc ttcaggacct atgtccagcc ccattttctc
     8041 gttcaacaga cattcggctt cctcctcgga gaaagcgagt tgctcggtgc gaatctcgtt
     8101 catctctccc ctggccctga gtcgccctat tggcagcggt gggtcagtgc ggctcgagat
     8161 gacgaggtgc acgttgtcgg ggacgtgctc cacgaagaag gccaccgact cgtgagcatc
     8221 ctcctcggtt acgaactggt agtcatcgag caccaatacg accctgtggg acaactcggc
     8281 gagctcgttt atgagcgagg gaagcgttgt tgcgacgaac ccctgcccca cagcgctcat
     8341 ccccacgaga acatcggtcc cgaagtcctc cccatctggt acagcccggt gcaacgcctc
     8401 cactatatgc ctccacagac ggatcggatc gttgtcctgc tcgtcgagag agacccatgc
     8461 aaaggacagg ttggctactt ctactcgacg ccagtgggcc aggagcgtcg tcttcccgta
     8521 gccggtcggg gcgctaatga tgctgagttt gcggtctgtg gcattctgga ggagatcgaa
     8581 catcctgcga cggaccagct gctcggagcg cggcaccggc gcccgcaact tgggcttgag
     8641 gacgatcatc gcagagtcac cgcttgagtc actcgaggct tggattacga tctcctctgt
     8701 tcgcgccgcg atctagagtc gcacactcac cgtaggggcg atcaggcgcc cttacggcgc
     8761 gacggcgccc ccaggacaag aacctctgct caccctccgt gatgacgtcg tcgactgtca
     8821 tcaggcccag ttcttgttct tcgtttttcc cattcttagc cgggacccgg tacatcccaa
     8881 cgatagcgaa ttatcgcata aacgacaata aggttgtata tctaaagctg caatccgcgt
     8941 cagagactcg gaatcgctac atttcgctag catcaagcgt tctgcatgag acgattgctc
     9001 ccgacctgta gaaggccaaa gtaccccaga aggtattggg cgacgatcct gtggcgtgta
     9061 cgtgcgccca gcaagaattc ccctagagga aacactccat tgggtgatga atccactttg
     9121 ctccgagact atgttggcgt acaggagaag tgagtagcgc tcgaacaggt aaagaaacgt
     9181 tgccgggacc atctgaggcg agtcgaataa ggccaagtaa aaggaggtca gggcggaagg
     9241 atagcgaacg ggccatcgaa aaagcgtggg ccccacgaga ctacagagag tgagaaagga
     9301 gagagaatga cgcagcacag cgaaccggtc ccgggagggg taggcagccc catggagagt
     9361 ccggccggtg caagtagggt agagcacaga cccggcatgc tcaccttcgc cgccgtcgta
     9421 atgttcatgg tggcgggctt cgaggcattg agcgccctcc ttgcgtttgc aggcagcgga
     9481 tggtgggtga ccgagacggg caacctggtg tacgcgaact tcgttctctg gggcatagtc
     9541 gacttgatca tcgcgttgat cgcgctgtat gcgggcatag acctcttgag aggaggagaa
     9601 ttcggccggg cgatggccta cgttttcgcc atcgtgggcg ccatcagggg gctattcgtc
     9661 atcccggcgg cgccggtgct tgcggtggta gtcatcgcgc tttgcgtgat ggttatctac
     9721 gcgcttgcca aacactccga ctactcccag agcgcctgac cgccctccgg tacagggtgt
     9781 tggccgacag gccgatccca tggtgacgaa cggttggtgg ccactgaagc gggtgggaga
     9841 tcctgcccgc ttcgcctcta ttagacaccc cccatcgtga agtaggggtc tcttgtattc
     9901 caccgaaacg ccaagcaacg cgatcttgag gagctacctg gggggaaggt ctcctccttg
     9961 gggagtcgat gtgcaccgca ttcgcgtacc cgatcagaaa ggagagaagt agtggcaacg
    10021 ctatcggtgc tgaagttcaa cgacccttac ggagcggagc gggtcatggt cgccctgcaa
    10081 ggcatgcagg agcggcagat gatcaccctg gaggatgcgg cagtagtcag ctggccgcag
    10141 ggtaacaaga agcccacgac ccgtcagcta cacagcatgg ccggtgcagg cgcaggctgg
    10201 ggcgcattct ggggtttcct gttcgggctg atcttcttcg tgcccttcct cggcgcggcg
    10261 atcggggctg ggatgggcgg actctcgggt gcgatggcag acgtcgggat cgacgacgac
    10321 ttcatcaagg aggttcgcga aaaggtcacg gagggaacct cggcgctctt cgcgcttacc
    10381 agcggagcga cggcgccgga caaggtaatc gacgagctaa agcaatacga cttcgagatc
    10441 atctctacca acctgcccaa ggagcaggag agcaagctac gtgaggcctt cgcacaagag
    10501 taattggggc gaaagcgcct tggagcgctc agagagttgt ccactccaga gcgggtggtg
    10561 aagaccgtcc cgaacggata gtagccttac cggcggagcg tttgcctgtt gtattggtgg
    10621 atccgagtag gggagcgcgc cttgacggaa gaagagacat ccttggcggg tttgcacagc
    10681 ctggtgatgt ccggtatcag gggagaatac ggtgactgat ctcctcgtgt tagccgccgt
    10741 catattcgtg ttcggcctcg tgtcgcggcg actggagggc accgttctca cggcgccgat
    10801 cttctttgtc gtcgccggcg cgctcctcgg tcctgcggta cttgggctgg tcgagtttgg
    10861 gctcgacgag cacaccgtgc tcttgttggg cgagatcgca ctcgccatcg tgctgtttac
    10921 cgatgctgcg ggcatcgact tctccgctct gcagcagtat cagggattgc ccctacgcct
    10981 tctcggtgtc ggtatgccgc taaccatcgc gctcgggacg accaccgcgg cccttctgct
    11041 c
//
LOCUS       DATMVF010000030         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_325190, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000030 DATMVF010000000
VERSION     DATMVF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325190"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VN178_01175"
     CDS             <1..297
                     /locus_tag="VN178_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWS79603.1"
                     /translation="MLRERNVALTLIDYPRMPRMEEATAGFSYVRLLGDRREFPSGHT
                     HPKKERDDDLRWWSDLVGRFLEEGKTVFAYANNHYQNHSPSTLDRFLEIRRKEI"
     gene            297..1076
                     /locus_tag="VN178_01180"
     CDS             297..1076
                     /locus_tag="VN178_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWS79604.1"
                     /translation="MAGPVLFSTGSLYPFGLDRVYGWISKAGYDGVEIMMDERWDTHQ
                     QDYLRDLAERHGLPILALHPPLRRGAWGLDAEETLVRVARLAGTMEVPIVVAHPPPPG
                     RPLERWKAGPLREARDQGVVVAVENMPQGRRAGMFGARRKSCYLPEHLADVGDVTLDT
                     SHAGASKVDILRAHSVLATQLRHVHLSDSNLEAGRDEHRLPGKGRLSLKPFLAALGAS
                     GYTGAVSLELKPWPLGAPDPGTIQERMRAARRFTREGLDTL"
     gene            1195..>2014
                     /locus_tag="VN178_01185"
     CDS             1195..>2014
                     /locus_tag="VN178_01185"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWS79605.1"
                     /translation="MVDERVGKLARLLVDYSIRAGEGDQVLVSGEVGATPLIKAIYAR
                     LLQVGATPVTQINLPGMQEIFFQEAQEIHYGEIPRVTRAIYEGVDAQVGILSPSNTMA
                     LANVDPEKQQALQMRNKPLSEMMLQKDRWVLTLFPTEALAQEARMGLSGYEEFAFEAM
                     GLNEDDPVRFWTEKAAEQDRLVERLEEARDIRIVGPDTDLTLSVEGRTFVNSAGRRNM
                     PCGEVFTGPIEDSASGTVYFGVPAAIAGREVSGARLRFEEGKVVEASAEKGEEYL"
BASE COUNT          368 a          613 c          701 g          332 t
ORIGIN      
        1 atgctccggg agcgtaacgt cgcgcttact ctcatcgact accccaggat gccgaggatg
       61 gaggaggcga cggcgggctt ctcctacgtg cgcctgctcg gcgacaggcg ggaatttcct
      121 tcggggcaca cccacccgaa gaaggagcgc gacgacgacc tcaggtggtg gtcggacctc
      181 gtcgggcgct tcctggaaga gggtaagacc gtcttcgcct acgccaacaa ccactaccag
      241 aaccactcgc cctcgacgct cgaccggttc ctggagatcc ggcgcaaaga gatctgatgg
      301 cgggccccgt tctcttcagt acgggttcgc tctacccttt cggcctcgac cgggtatacg
      361 gttggatctc aaaggccggc tacgacgggg tcgagatcat gatggacgaa cgctgggata
      421 cccaccagca ggactatctc agggacctcg ccgagaggca tggcctcccg atcctggccc
      481 tccatcctcc gctacgacgc ggcgcctggg ggctcgatgc cgaagaaacc ctggtgcggg
      541 tcgcgagact ggcgggtacg atggaggtgc ccattgtagt ggcgcatcca ccaccgccgg
      601 gcaggccgct ggagcgttgg aaggccggac ccctgcgcga ggcgcgggac cagggtgtcg
      661 tcgtggccgt cgagaacatg ccccagggcc gccgggctgg catgttcggt gccaggcgga
      721 agagctgcta cctgcccgag cacctcgcgg acgtgggcga tgtgacgctg gataccagtc
      781 acgccggggc cagcaaggtc gatatcctca gggcccactc ggtcctggcg acccagctcc
      841 gtcacgtgca tctgtccgac tcgaacctgg aggcgggccg tgacgagcat cgcctgccag
      901 gcaagggccg gctctcccta aagccctttc ttgccgcgct gggggcgagc ggttatacgg
      961 gtgccgtgag cctggagctc aagccctggc cgctcggcgc cccggatcca gggactatcc
     1021 aggagaggat gcgcgctgcg cggcggttta ccagggaggg tctcgatacg ctctagtcag
     1081 gggggcttgc ggtctctagc ctcactgccg gcgtggaact cttcccctgg catgcccgcg
     1141 agggacgagg cggatgataa gctctccggg atcttcccca gaaaggagct agcgatggta
     1201 gacgaaaggg tcggcaagct tgcacggctg ttggtggact attctatccg ggccggggag
     1261 ggggaccagg tcctcgtctc cggggaggtg ggcgccaccc ccctgataaa ggccatctat
     1321 gcgcggctgc tgcaggtggg cgccactccg gtaacacaga tcaacttgcc ggggatgcag
     1381 gagatcttct tccaggaggc gcaagagatt cactacgggg agatcccgag ggttacgcgc
     1441 gccatctacg agggcgtgga cgcccaggtc ggcatcctgt cgccgagcaa taccatggcg
     1501 ctcgcgaacg tcgatcccga aaagcaacag gcactccaga tgcgcaacaa acccttgagc
     1561 gagatgatgc tccagaagga ccgctgggtg cttacgctct tcccgacgga ggcgctggcg
     1621 caggaggcgc gcatgggcct ctccgggtac gaggagttcg cgttcgaggc tatgggactc
     1681 aacgaggatg accccgtgcg cttttggacg gagaaggccg ccgagcagga ccgcctggta
     1741 gagaggctcg aggaggcgcg agatatccgt atcgtcggtc ccgacacgga cttgacgctc
     1801 tcggtcgagg gacggacgtt cgtgaactct gcgggaaggc ggaacatgcc ttgcggcgag
     1861 gtctttaccg ggccgatcga ggactcggcg agcggaacgg tctacttcgg cgtacccgcc
     1921 gcgatagcag ggcgcgaggt ctcgggcgca cgacttcgtt tcgaagaagg gaaggtcgtc
     1981 gaggcgagcg cggagaaggg ggaggagtac ctaa
//
LOCUS       DATMVF010000031        14379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_187989, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000031 DATMVF010000000
VERSION     DATMVF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14379
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187989"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..364
                     /locus_tag="VN178_01190"
     CDS             218..364
                     /locus_tag="VN178_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79606.1"
                     /translation="MDGTGVRLEFVTPTFIRFKVDVSPQAGQLTYAGKRAVFGLGRYR
                     IRET"
     gene            complement(462..1850)
                     /locus_tag="VN178_01195"
     CDS             complement(462..1850)
                     /locus_tag="VN178_01195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWS79607.1"
                     /translation="MRRVIWLNLTHHRGLRGLAVLLGILGSLAALSAVGKLASSAEAL
                     QGSKGPTQRTQGEVVGASISHPTRWFLERERYTYDQTYGFTLWRPDSGALHDHGGTPA
                     VRVALAYGLRPGQIKATVQKKLRAYPDLPITRQEVSVAEKGHRGVAVGTIPGSTPSTE
                     VYVPVNGRVYQINVYGNRFGSDDKKLLSALRFEPPSRPVSSLGLPVANSAKSLYGAGD
                     PKRAERGRAMHKATTEGKTTFAATSLGEETLIDEGCWQADPDFFFQTQYGSTANADPK
                     DNIPTGWSIVGSPNYWNEYTHGGLEGYGRCNRRHFTNDKFAVDYPLNRGDPIYSPFGS
                     GTVTFAGRNITHKDYGLFVSIEGANGSYVNLSGHLSLLAPGIKEGAEVDRHTVIGFAG
                     KTGANKFPVGSVHLHQAYYRNPTYTPDGSPFGGAGLKVVDHHYYRDDNGVYRYGWVER
                     REIKYKGSWISF"
     gene            complement(2083..3609)
                     /locus_tag="VN178_01200"
     CDS             complement(2083..3609)
                     /locus_tag="VN178_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HWS79608.1"
                     /translation="MEPSILSLLPPVVAIGLAIATRRILLSLGIGIILGALMYSQWDI
                     LASISLVAEVAGGLIIAEGAIAEEMYILAFVVMLGILTTKVRTRFQAQLVPYILGLVI
                     FFDDAFSCLVGGNVSRTITDKYRISRAKLSYIVDSTAAPVIILMPISGWAAFIAATMT
                     GILNRNEVTAFSGYEAFLSSIPTNYYAITAILFVFAVAFFGLNFGPMRKHERRAMEAG
                     VLVDTSHGAVPGEADRDLPTRKDGRVSDLVVPVATLIGVTVAGALWIGISNTDGAITP
                     MQILANTDVILSLFYGGLIACAVSGAMLLLKRTPANQVATAGLSGIRSMLMAAAVLFL
                     AWMTAEIIALLGIGEYLSGLINTGLAFSLLPALLFVLACFISFSMGSTFGTFGLILPI
                     AAEIVVSVDLSLLIPAFGAVLAGAIFGDHTSPLSDTTILSSIGSGIQLIDHTTTQIPY
                     ALVCAVASLVGFIVLGFTRSTTVGLLTALAVLAVAIFVLRSTSVSETPRESEAGGITG
                     "
     gene            3939..4721
                     /locus_tag="VN178_01205"
     CDS             3939..4721
                     /locus_tag="VN178_01205"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HWS79609.1"
                     /translation="MSPRREFGQSLSRKKPAVVLLLAIILLLAAACGGQASDDSSNGS
                     GKREASRTQEQDATSVASSENSVVEETAVGKAGPSGEEAELARAEVGKEEISNMMPAG
                     AEKPDAARPLPENPPSGIEVYPATTNHTVEGPIDYERHPPTNGDHDPLWQNCGFYEEP
                     IEDRHAVHSMDHGVVWITYRSDLPERQIESLRPYGKEDYVIVSPYPGQDSPVIATSWR
                     VQLELNDSDDPRLRRFVNQFRISELAPLSGNRCTLGVGSPGG"
     gene            complement(4813..6165)
                     /locus_tag="VN178_01210"
     CDS             complement(4813..6165)
                     /locus_tag="VN178_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HWS79610.1"
                     /translation="MQQTKFVLEEKAIPESWYNLVPDLPFQLEPPLNPATMEPVGPEA
                     FAPIFPQAIIEQEVTPEPNVPIPEEVREIYSLWRPTPLFRARRLEKFLDTPAHIYYKY
                     EGVSPPGSHKANTAVPQAYYNREEGVRRLTTETGAGQWGSALAFACSVMGLDCTVYMV
                     RVSYDQKPYRRIMMETYGAEVHSSPTDLTQAGSNILEEHPDSPGSLGIAISEAIEDAV
                     KNDDAKYSLGSVLNHVLLHQTVIGQEALEQMELAGEYPDVVVGCVGGGSNFGGVAFPF
                     IGENLKNGKNTRIVAVEPASCPTLTKGRYTYDFGDTAGTTPMMKMYTLGHSFVPSGIH
                     AGGLRYHGDSPIVSALAHEGLIEARAYAQNPTFEAGVTFARTEGIVPAPEVTHAIKAT
                     IDLALEARESGEQQTILFNLCGHGHFDFSAYQQYLAGELVNLEHSEEEIEVAVAGIPG
                     "
     gene            complement(6311..8083)
                     /locus_tag="VN178_01215"
     CDS             complement(6311..8083)
                     /locus_tag="VN178_01215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWS79611.1"
                     /translation="MAYAFARYLKIRGAWGSSWSADGRRLSFLTEITGVPQVWEVSSE
                     APSWPEQLTFYEERISGALYSPTENRLLFGMDAGGNERSQLFLLDEGRVTDLTRAPDA
                     IHYSGGFSPEGSRIAYTATRRNGTDFDVFVQELPDGEPEMVWEVSGYHTVVDWAPDGS
                     ALIVSRHHSNVNNDLYRLNLDTGEATLLTGHEGDARFSSANVTPDGGSLFLATDRDGE
                     FLRLARLDFTTLKLEYLTPDDRDVEGVELSRDGRYLVASRNVDGYSDVLLFDGRGRRM
                     PDPRMPEGIVGGFQFSPDSGRLAFTLTAPERNPDVWVVDLPDGDARRLTRSSTAGIPP
                     RSFRKPRVVRYTSFDDRRIPALFYEPEAEDAPVIVNVHGGPESQSRPAFAPVTQYLLG
                     RGYAVFFPNVRGSTGYGKAYTHLDDVGLRMDSVEDLAYAVHWLKEGGHRSIAVMGGSY
                     GGFMVLAALTAYPELWSAGVDIVGIANLVTFLENTGSYRRSLREPEYGSLENDRELLE
                     SMSPIHKAGEITAPLMVIHGKNDPRVPVGEAEQIVERVKVNGGKVEYLLYEDEGHGLA
                     KLKNRLDAYPKIAAFLDEHLGPTR"
     gene            8318..8758
                     /locus_tag="VN178_01220"
     CDS             8318..8758
                     /locus_tag="VN178_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79612.1"
                     /translation="MVLRRLRSIFGGPERPTGEPSPLYGLEEEERAKIGTEEDPLRNF
                     EAAMQRNEEAELAVKGGDTERAIKLYEISIREEFVGSHPYERLASVYESRHNLSEALR
                     VSEAFTKLAASGKMPRGAQRSADRKLPEFEARIQRYRRSLDKRR"
     gene            8963..9328
                     /locus_tag="VN178_01225"
     CDS             8963..9328
                     /locus_tag="VN178_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWS79613.1"
                     /translation="MKSCLKMLLVAGAFAVSLAVFQDDALADTQLASWYGPGFEGNIT
                     ASGDVFEPYTEYTAASPYLPFGTKLLVSYGTESVVVEVTDRGPYSGGRDLDLSQVAAE
                     DIGLIYTGVDYVDVEVLSY"
     gene            9549..10070
                     /locus_tag="VN178_01230"
     CDS             9549..10070
                     /locus_tag="VN178_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWS79614.1"
                     /translation="MEETLQGIRFFEFLTEEEREEFAEVSELVSFGPGENIIEEGADQ
                     ESLFVLVSGAVEVRKGLPGGRSRLLAEIDASRGPTVVGERGLLGESGASATVMALGEV
                     GAIRIPRDTFRRMVAEGRPAAFKLSYRIARTLARRLTRLDEEVVEAIRELENRGETDM
                     EAFRDRLITDWSV"
     gene            10083..10607
                     /locus_tag="VN178_01235"
     CDS             10083..10607
                     /locus_tag="VN178_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWS79615.1"
                     /translation="MRQTPDLSDVPIFKHLDEDERAEFEELSEPVRFQAGQMIFEEGA
                     PEERLYVITSGTVEVYKSILPGRRQRLATIEAPSVVGEMGLLTEPRAAASVEAITMVE
                     AHGVGRDRLLEMLDADSPAACKLVYEIGRTLAERMAATNETVAEVIFRLENTAGRSED
                     IDVFRDQLIQDWSF"
     gene            complement(10721..11314)
                     /locus_tag="VN178_01240"
     CDS             complement(10721..11314)
                     /locus_tag="VN178_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79616.1"
                     /translation="MDIVTLAQVVTSVTNLLFVIVLAAGYYFTWRVSQTTLEEMRAQR
                     TAMGRPLVIVQEDYESLPELDVTIRNVSEGAARDIQFEFSVPIESSNGFVVSDLPYFK
                     YGLDFLPPNGEITCYWDELDSLLPFLESKDLRSGIHVTVRYKDLTGDSYQSQWRLNPF
                     LYKDTRLVRRKGIGDLVNAVERLALSKDGRAERHIQQ"
     gene            complement(11532..11690)
                     /locus_tag="VN178_01245"
     CDS             complement(11532..11690)
                     /locus_tag="VN178_01245"
                     /inference="COORDINATES: protein motif:HMM:NF045043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79617.1"
                     /translation="MTDDKHEVQGEAEPLNWACTNCGYRVEGELPEKCPDCGADREMF
                     DEVPVPGY"
     gene            11872..12696
                     /locus_tag="VN178_01250"
     CDS             11872..12696
                     /locus_tag="VN178_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWS79618.1"
                     /translation="MIFPIFSVQLGAALAKSFFDSLGPGGMVFLRIAFAALVLFLLVR
                     PKLGGYDRAGYLVAGLFGLTLAGMNLSLYLALDRIPLGVAVTLEFVGPLGVAVAGSRR
                     LLDLLWVVLAAAGIILLAPLGVLGGMDLDPVGVAFALLAGCLWACYILLSVRTGSAFP
                     GGTGLVIALCVGTLVLFPFGIVGAGYALLDPKFLLAGFGVAVLSSAIPYSLELEALRK
                     LPARVFGVLMSLEPAVAALAGLVVLGERLEMRAVAAIIFVTIAAAGASLFGRRDEG"
     gene            12829..13932
                     /locus_tag="VN178_01255"
     CDS             12829..13932
                     /locus_tag="VN178_01255"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWS79619.1"
                     /translation="MSARVLTLRELNRATLDRQMLLDRQSIPALDAVERLAGLQAQVP
                     SPPYVGLWTRLKDFRREELTRLMEERQVVRATLMRATLHLMAAEDYLLLRPALQPALT
                     RSMNSIAGKRLEGLDLDRIVGIAREYFESEPRPFADFRLLLAELEPERDQSALAYAVR
                     TQLPLVQVPTGGVWGYSGKVPFITAEKWLGRDLSGSEDPRGLVLKYLAAFGPATVRDV
                     QTWSGRIRLNESVEEIKPELRIFRDENDNELLDLPDAPLPPGDIPAPTRFVPDYDNLV
                     LSHADRGRVISDEHRKKVFLSAARVRATFLIDGFVRGVWKVEKTRRAATLVIEPFERL
                     SRGDRDSLSEEGERLVRFLGEPQGAETFDVRFV"
     gene            14072..>14379
                     /locus_tag="VN178_01260"
     CDS             14072..>14379
                     /locus_tag="VN178_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79620.1"
                     /translation="MRFHLIVLALGTFAIGTGSFVLAGPLEGVARDLSVSVGTAGNLV
                     TVFAITYAVSSPVLVTLVGSAAPRRVLVAAMAVFVLGNAAAVAAPTFGLLVLCRILAA
                     "
BASE COUNT         2658 a         4361 c         4608 g         2752 t
ORIGIN      
        1 cgccaaactt caattcactg aattcgtccg gggcgccggg aggcttaggt agatcagccg
       61 cctcacctcc cgccgtcccc gaacccccgt gtcgaggatg aggccgcacc cgaagctcat
      121 cgcgacgacg atcatcaggc cggtgattag gatcgcggtc gggaagcgcg ggacgaggcc
      181 ctaatccacg caacactcat ctaggagccc ggctacaatg gatgggacag gggtgcgcct
      241 ggagttcgtg accccgactt tcatcaggtt caaagtagat gtctctcccc aagccgggca
      301 actgacgtac gccggcaagc gtgcggtctt cgggctggga cgttaccgga taagggaaac
      361 ctgagcctcc gggcctccac cagcgcagcc tccaccgcct gaatcgagag gagcgctttg
      421 ttggtgaacg tagcggtcgc tgacggcaga cggatggttc ttcagaagct gatccaggag
      481 cccttgtact tgatctcccg tctctcgacc cagccgtatc tgtagacgcc attgtcgtcg
      541 cggtagtagt gatggtcaac taccttgagt ccagcgcccc cgaatggcga gccgtcgggg
      601 gtgtaggtgg ggttgcggta gtaggcctgg tgcaggtgta cagatccgac cgggaactta
      661 ttggcgcccg tcttgcccgc aaagccgata acggtgtgcc tgtcgacctc agctccctcc
      721 ttgatcccgg gggcgaggag gctcaggtgc cccgagaggt tcacatagct gccgttggcg
      781 ccttcgatgg agacaaagag gccgtagtct ttgtgggtga tgttgcggcc tgcgaaggtc
      841 accgtgccgc tcccgaaggg cgagtagata ggatctccgc ggttgagggg gtagtccacg
      901 gcgaacttgt cgttggtgaa gtgtcgcctg ttacagcgac cgtacccctc gagaccgccg
      961 tgggtgtact cattccagta gttggggctg cctacgatgc tccaacccgt ggggatatta
     1021 tctttggggt cggcgttggc cgtggacccg tactgcgtct ggaagaagaa gtcggggtcg
     1081 gcctgccagc aaccttcatc gatcagagtc tcctccccca gcgacgtggc cgcgaaggtc
     1141 gtcttcccct ccgtagtcgc cttatgcatc gcccgccccc gttcggcgcg cttgggatcg
     1201 cccgccccgt agagggattt cgcagaattg gcgacaggta gcccgagcga gctgaccggc
     1261 ctggatgggg gctcgaagcg caaagcggat agcaatttct tgtcgtccga accgaatcta
     1321 ttcccgtaga cgttgatctg gtacacccgg ccgttcaccg gcacgtagac ctccgtggag
     1381 ggcgtgctgc cagggatggt cccaaccgcc acgcccctgt gccccttctc ggcgacgctg
     1441 acctcctggc gagtaatggg gaggtcgggg taggcacgga gcttcttttg gaccgtcgcc
     1501 ttgatctgtc ctggccggag accgtaggcc agcgcgacgc gcaccgcagg cgtcccgccg
     1561 tggtcgtgca gggcgccaga gtccggcctc cacaaagtga agccgtaggt ttggtcgtag
     1621 gtgtaccgtt cacgctcgag aaaccacctg gtgggatggg agatcgaggc ccctactact
     1681 tcgccttgcg tcctctgggt tggccccttg ctcccctgga gagcctcggc cgaagaggcg
     1741 agcttgccca cggccgacaa tgcggcaagc gaccccagga tcccaagtaa gacggcaaga
     1801 cctctcagac ccctatgatg ggtgagattc aaccaaatga ctcgccgcac ggtggtgtcc
     1861 ccctccgata gtacttcttc cccttgatct taggtctcgc atattaagtt gcttgcggac
     1921 cggagtcaat ggctactccg ccaagccgcc aaatccctgt ggacgctctc cggcgcgacc
     1981 caaagccctc tcgggccggg tgaaaaacta cccggcccga gtgggaaccg cctaaagaga
     2041 gttcttgcga agggtcttgt ctccgccgtg agacttcggg tgctatccgg tgatcccccc
     2101 ggcttcagac tcacgcgggg tctcactgac ggacgtgctc ctcagtacga agatagcaac
     2161 agccaggaca gccagtgccg tgagcaggcc gaccgtggtg ctcctggtaa acccgaggac
     2221 gatgaatccg accagtgatg cgacggcgca gacgagcgcg tagggtatct gtgtcgtggt
     2281 gtgatctatt agctgtatgc cagacccgat agaagacagg atcgtggtgt cggacagagg
     2341 ggatgtgtgg tccccgaaga tggcaccagc caacacggcg ccgaaggcgg ggatcaacag
     2401 cgagagatct accgagacca cgatctcggc ggctatcggt aggatcaggc cgaaggtgcc
     2461 gaacgtgctc cccatcgaga aggagatgaa acaggccagg acgaacagga gagccggcag
     2521 cagagagaac gccaggccgg tattgatcag cccagacaga tactccccga tccccagcaa
     2581 ggcgattatc tcggcggtca tccacgccag aaatagcacc gcggcagcca taagcatcga
     2641 cctgatgccc gatagcccgg cggtcgccac ctggttggcc ggggtcctct tcaacagcag
     2701 catcgcccca gaaacggcgc acgcgataag gccaccatag aagagggaga ggatgacgtc
     2761 ggtgttcgcc agtatctgca tcggggtgat cgccccatcg gtgttggaga tcccgatcca
     2821 gagcgctcct gccacggtga caccgatgag cgtcgccaca ggcacgacca ggtcgcttac
     2881 cctgccgtcc tttcgcgtcg gcaggtccct atcggcttca ccagggacag ccccgtgcga
     2941 ggtgtcgacc aggactccgg cctccatcgc gcgcctttca tgcttgcgca tcgggccgaa
     3001 gttgagcccg aagaatgcaa cggcaaatac aaacaagatc gcggtgatcg cgtagtagtt
     3061 cgtggggatc gacgacagga acgcttcgta gccggaaaat gcggtgactt cgtttctgtt
     3121 cagtatcccc gtcatggtgg cggcgatgaa ggcggcccag ccggagatcg gcatcaggat
     3181 gatgacgggg gcggccgtgg agtccacgat gtaagagagc ttcgcccgtg agatacgata
     3241 cttgtcggtg atcgtcctgc taacgttccc gccgaccaga caactgaaag catcatcgaa
     3301 gaagataacg aggcccaaga tgtaagggac cagctgggcc tggaagcgtg tacgtacctt
     3361 cgtggtaagt attcccagca tgacaacgaa ggcgaggatg tacatctcct ccgcgatggc
     3421 gccttcggcg atgatcaggc ctcccgctac ttcggcgacg agcgagatgc tggcgaggat
     3481 atcccattgg ctgtacatca gcgcgccgag gatgatcccg atccccaacg acagcaggat
     3541 cctcctggtg gctatagcca gaccgatcgc gacgaccggc ggtagaagtg acagtataga
     3601 cggttccatt ggcaaggcct cctgatggtg cgaaggattg cttcgctgac ctccgagctt
     3661 acggtctgtg ccacgacctt acgccgtgga ctcgagccta ctgagatctc tgcgtcaggc
     3721 cacttttacc atcagtacct acctcctccc cgaaggtacg tacccacaca tgcaatagta
     3781 cccaatgatg catgacccaa acttcctgca aatcggcccc acgagaactg gcctcgagcc
     3841 cggttgtccg agaacaaccg cgcggccaga tccatagtac cttcagcttc gtagatacct
     3901 ttgcctgaaa acacaggaag gtcgcacctt tgagcgccgt gagcccgaga agagagttcg
     3961 gacagagtct cagcagaaag aagcccgccg tcgtgctcct gctcgctatc atcttgctcc
     4021 tcgcggcagc atgcggaggg caggcctcgg acgattcctc caacggttcg gggaaacggg
     4081 aggcttccag gacccaggag caggacgcca caagcgtcgc ttcctcagag aatagcgtcg
     4141 tcgaagagac cgctgtaggg aaggcagggc cttccggcga ggaagccgag ctcgcgcggg
     4201 ccgaggttgg gaaagaggag atctccaaca tgatgccggc aggagcggag aagcctgatg
     4261 cggcacgacc tctgcccgag aaccctccaa gtgggataga agtctatccg gccacaacca
     4321 accataccgt cgagggtccc atagattacg aaaggcaccc gccgaccaac ggggaccacg
     4381 atccactgtg gcagaactgc ggcttctacg aggagcccat cgaagacagg cacgccgtcc
     4441 acagtatgga ccacggcgtg gtctggatca cctaccgctc cgaccttcca gagcggcaga
     4501 tcgaatctct gcgtccctac ggaaaagagg actacgtcat agtgagcccg taccccggcc
     4561 aggactcgcc cgtgatagcc acctcatgga gggttcaact ggaactgaac gactcggacg
     4621 acccacgcct aagacgcttc gtaaaccagt tccgcatctc cgagctagct cccctctcag
     4681 gcaaccgctg caccctgggc gtgggcagcc cggggggata gaacccgagg ctgcccattc
     4741 ccagttccgt tcgcacggaa atcctgaaac ctgatgccgt aatcagcttg ttggagcggt
     4801 tcgcgaaccg ccctaaccag gtatgccggc gacggccacc tctatctcct cctccgagtg
     4861 ctccaggttg accaattctc cggcgaggta ctgctggtag gccgagaagt cgaagtggcc
     4921 gtggccgcag aggttgaaga ggatggtctg ctgttctcct gactctctgg cctcgagcgc
     4981 gaggtctatg gtggctttga tcgcgtgcgt cacctcgggc gctggcacga tgccctccgt
     5041 cctcgcgaac gtgaccccgg cctcgaaggt ggggttctgg gcgtaggcac gcgcctcgat
     5101 cagcccctcg tgtgccagcg ccgagactat gggggagtcg ccgtggtaac gcaagccccc
     5161 cgcgtggatg ccactaggca cgaacgagtg cccgagcgtg tacatcttca tcatcggggt
     5221 ggtgcccgcg gtgtctccga agtcgtaggt gtagcggccc ttggttagcg tcgggcaaga
     5281 ggcgggctcg acagccacga tacgcgtgtt cttcccgttc ttcaggttct cgccgatgaa
     5341 ggggaaggcg acgccgccga agttcgagcc gccgccgacg cagccgacca ccacgtcggg
     5401 gtactcgccg gccaactcca tctgctccag cgcttcctgc ccgatcacgg tctggtgcaa
     5461 cagcacgtgg ttcaggacgc tccctagcga gtacttcgcg tcgtcgttct tgaccgcgtc
     5521 ttcgatcgcc tcgcttatgg ctataccgag ggaacccggc gagtcaggat gctcttcgag
     5581 gatgttgctg ccggcctgcg tgaggtccgt tgggctcgag tgaacttcgg ccccgtaggt
     5641 ctccatcatg atgcgcctgt agggcttctg gtcgtagctg acgcggacca tgtagaccgt
     5701 gcaatcgagg cccatcacac tacaggcgaa ggcgagtgcg gagccccact ggcccgctcc
     5761 ggtctctgtg gtcaagcgcc tgacgccttc ttcacggttg tagtaggcct gggggacggc
     5821 ggtgttcgcc ttgtggctgc ctggcgggct gacaccttcg tacttgtagt agatgtgggc
     5881 cggggtatcg aggaactttt ccaggcggcg ggcgcggaag agtggggtcg gacgccagag
     5941 gctgtagatc tcacgcacct cttcggggat gggaacattg ggttccggcg tcacctcctg
     6001 ctcgataatc gcctgcggga agatcggggc gaacgcctcc ggccccacag gctccatcgt
     6061 ggccggattc aagggcggct cgagctggaa aggcagatcc ggcaccaggt tgtaccagct
     6121 ctcggggatg gccttctcct cgaggacgaa cttcgtctgt tgcatcccgc acctcccata
     6181 ctcggctctc agagatcaga caccagctta cagcttttcg cgacgcaggg ttgtgggaga
     6241 tcgagcgccc acctcgacgt gccggcaaag ctgaccggct tactgcagca ggactagcgc
     6301 agacgcttgc tcacctcgta ggtcccaggt gctcgtcgag gaaggctgct atcttcgggt
     6361 aggcgtcgag gcggttcttc agtttggcga ggccgtggcc ctcgtcctcg tagaggagat
     6421 actcgacctt gccgccgttc actttcacgc gctcgacgat ctgctcggcc tccccgacgg
     6481 gcacgcgggg atcgtttttg ccgtggatga ccatgagagg ggccgtgatc tccccggcct
     6541 tgtgtatggg gctcatggac tccagtaact cgcggtcgtt ctccagcgag ccgtattcgg
     6601 gctcgcgcag gctccgccgg taggagcccg tgttctccag gaaggtgacg aggttggcga
     6661 tacccactat gtccacccct gcactccaga gctcagggta tgcggtgagt gcggcgagca
     6721 ccatgaaacc cccgtaggag cctcccatta ccgcgatact ccggtggccg ccctctttga
     6781 gccagtgcac cgcgtatgcc aggtcctcga cggagtccat cctcagcccc acgtcgtcga
     6841 ggtgggtgta agccttaccg tatcccgtcg atccgcggac gttcgggaag aacacggcgt
     6901 agcccctgcc gagcaggtac tgcgtcacgg gggcgaacgc gggtcgtgac tggctctcag
     6961 gcccgccgtg gacgttcact ataacgggcg cgtcctctgc ttcaggttcg tagaagaggg
     7021 ccggtatcct gcgatcgtcg aagctggtgt agcgcacgac ccgtggcttg cggaagctgc
     7081 ggggcgggat gcccgccgtc gaggagcggg taaggcgccg cgcatctccg tcgggcaggt
     7141 cgacgaccca gacgtcgggg ttgcgctcag gggccgtgag ggtgaaggcg agccgtcctg
     7201 agtcgggtga gaactggaag ccgcccacga tcccttccgg catccgtggg tcaggcatcc
     7261 ttcgtcccct cccgtcgaag agcagtacat ctgagtagcc gtccacgttg cgactcgcga
     7321 cgaggtagcg gccgtccctc gaaagctcga cgccttcgac gtcccgatcg tccggtgtga
     7381 ggtactcgag tttcagcgtc gtgaaatcga ggcgggccag acgcaggaac tccccgtcgc
     7441 ggtccgtcgc caggaagagg cttccgccat cgggcgtcac gttcgcggag gagaacctgg
     7501 cgtccccctc gtgcccggtc aggagcgtcg cctcgccggt gtcgaggttc aaacggtaca
     7561 ggtcgttgtt cacgttggaa tggtggcggg agacgatcag ggcgctcccg tcaggagccc
     7621 agtccaccac cgtgtggtag ccggagacct cccagaccat ctcgggctca ccatccggca
     7681 gttcctggac gaagacgtcg aagtccgtcc cgttgcgccg ggtagccgta tacgcgatgc
     7741 gacttccctc gggcgagaag cctccggaat agtgaatggc gtcaggggcg cgggtaaggt
     7801 ctgtgacacg gccttcatct agaaggaaga gctgtgaacg ctcgttgccg ccggcgtcca
     7861 tgccgaagag gagcctgttc tcggtcggcg agtaaagggc gcccgagatg cgctcctcgt
     7921 agaaggtgag ttgctcgggc caggagggag cctcgctgga gacctcccat acctggggca
     7981 cgccggttat ctccgtcagg aaagagagtc tccggccgtc ggcagaccaa ctcgagcccc
     8041 aggcccccct gatcttgaga tagcgggcaa acgcgtaagc caaagtgggt cccttccaag
     8101 taggtgccaa agagcagaac aagagcatgt tagcatttca gcatgtcagc acttcagttg
     8161 gttagctcgt ttggagagcg gcccgaattc gttttgccat gtggtggata agggtgcgca
     8221 acgtttgcca cggagccagg ctgtcggaca cttgctgaaa tgctgactag ctgacatgct
     8281 gaagtgctga gcgaccgaag ggagcgtagg gaaaggcttg gttctaaggc gtctgaggag
     8341 catattcgga gggccggagc ggcctacggg cgagccgtcc cccctctacg ggctcgaaga
     8401 agaagagagg gcgaagatcg ggacggaaga ggacccgctt cgtaacttcg aggcagcgat
     8461 gcaacgcaac gaggaggccg agctggccgt caagggcggc gacaccgaac gggccatcaa
     8521 gctctacgag atcagcatcc gcgaggagtt cgtcggaagc cacccttacg agcgcctcgc
     8581 ctctgtctac gaaagccggc ataatctcag cgaagctctc agggtatccg aagccttcac
     8641 aaagctcgcc gccagcggaa agatgccccg cggagcccaa cgctccgccg accgcaaact
     8701 cccagagttc gaggcccgca tccaacgcta cagacgctcg ctcgacaaac ggcgataaac
     8761 ccagaagccc agaacgcccc tcatccagca agaaaaccac cgtatgtagc caagaggcgc
     8821 ctctgacacc acataatgta ggtcagccgt ctatgtaact agttcacaaa tagacgcaaa
     8881 aaacgatatg tagcgggttg taactcagtt gtaacgtagt aaggtcttgg gcttgttgtg
     8941 caagcgatct ggagggggac tcttgaagtc ttgtctcaag atgctgttgg tggctggcgc
     9001 tttcgccgta tcgctggccg ttttccagga tgatgccctg gccgacaccc agctggcgag
     9061 ctggtacggg cctgggttcg aaggtaacat tacggcgagt ggggacgtct tcgagcccta
     9121 caccgagtac acggcggctt cgccttacct gcctttcggt acgaagttgc tggtgagcta
     9181 cgggaccgag tccgtggtcg tcgaggtcac cgaccgtggg ccgtattcgg gtggcaggga
     9241 cttggatctt tcccaggtag ctgccgagga catcgggctt atctacaccg gtgtcgacta
     9301 cgttgacgtc gaggtcctca gctactaaga ggtcctcaac caggggtctt cgccggacat
     9361 aacggcgaga cacgagagcg ggcgcggttc tttggccgcg cccgctttcc attgggctaa
     9421 tcgtcacacc cttcggtatc ccgagtaaag aagcaggtct ttcgatgggc ttcttgcacg
     9481 gtgcgccggg acggaatgcg cacgtataat ctcgctagcg gcttcgaggc tgaaggggtg
     9541 atgggaagat ggaggagacg cttcagggga tcaggttctt cgagttcctg accgaggagg
     9601 agcgggagga gttcgcggag gtatcggagc tcgtctcctt cgggccgggc gagaacatta
     9661 tcgaagaggg cgcggatcag gagagtctgt tcgtgctcgt ctcgggcgcg gtcgaggtca
     9721 gaaagggatt gccgggagga aggagcaggc tcctggccga gatcgacgcc tcgcgtggac
     9781 ccaccgtcgt gggagagagg gggcttctcg gcgagagcgg ggcgtccgct acggtcatgg
     9841 ccttgggtga ggtcggggcg ataaggatcc cacgcgacac cttcaggcgc atggtcgcgg
     9901 aaggcaggcc tgccgccttc aagctctcct accgcatcgc ccgcaccctc gcgaggaggc
     9961 ttacgcgtct ggacgaggag gtggtcgagg cgatccggga gctcgagaac aggggggaga
    10021 ccgacatgga ggccttccgc gaccgactga taacggactg gtccgtgtag ggggttctat
    10081 ttatgcgaca gacgccagac ctcagcgacg tgccgatctt caagcatctg gatgaggacg
    10141 agcgggcgga gttcgaggag ctttcagagc cggtgaggtt ccaggccggg cagatgatct
    10201 tcgaggaagg cgccccggag gagcggctgt atgtgatcac ctctggaacc gtcgaggtct
    10261 acaagagtat cctccccggt cgccggcaac gcctggcgac catcgaggcg ccttccgtgg
    10321 tcggcgagat gggcctcctc actgaaccca gggccgccgc gagcgtcgaa gcgataacca
    10381 tggtcgaggc gcacggcgtt ggtagagacc ggctcctcga gatgctggat gccgactccc
    10441 ccgcagcctg caagctagtc tacgagatcg gccgtaccct cgccgaacgt atggccgcga
    10501 ccaacgagac cgtcgccgag gtcatcttcc gcctcgagaa caccgcaggg agatccgaag
    10561 atatagacgt cttccgggac cagctcattc aggactggag tttctaaggt tgtagggacg
    10621 ggtttgaaac ccgcccctac ggcttcaggt ttcaggcatc aggggttcgc ggcgcaacct
    10681 aatggcctga aactctgcgc tctacgcgcc tggctgttgg tcactgttgg atgtgtcttt
    10741 cggcccttcc gtctttgctc aatgcaaggc gctccacggc gtttacgaga tctcctatgc
    10801 ctttgcgcct tacgaggcgg gtatctttgt agaggaacgg gttgagcctc cactggctct
    10861 ggtatgagtc acctgtgaga tctttgtacc tgacggtgac gtggatgccg ctcctcaggt
    10921 ccttggactc caaaaacggc agcagagagt cgagttcgtc ccagtagcag gtgatctcac
    10981 cgttcggcgg caggaagtcg aggccgtatt tgaagtaagg caggtccgag acaacgaagc
    11041 cgttggagct ctcgatgggt acggagaact cgaactgtat gtccctggcc gcaccctcgc
    11101 tcacgttgcg gatggtcacg tcgagctcag gcaggctctc gtagtcctcc tgcactatta
    11161 ccagcggacg acccatcgcc gtgcgctggg ccctcatctc ctcgagcgtg gtctggctta
    11221 cccgccaggt gaagtagtat ccggcggcga gcacgatcac gaacagcaag ttggtcaccg
    11281 aggtgacgac ctgcgccagg gttacgatgt ccacgggccg tacattatcc gagacgacaa
    11341 ccggaaacaa gcgtcgctat aggtttcagg tttcaggcta caggcatcag gtaaaggccg
    11401 ttgtggccgc agcctggcag cgttgaagtg tcggggacta ctcgccgata ctcctcttcc
    11461 ggagacgctt taacgcctga cgctttgccg cgagaggcgt tacctgaagc ctgaaacctg
    11521 atgcctgaca cctaataacc cggcaccggc acctcgtcga acatctccct gtcggcgccg
    11581 caatccgggc acttctcggg tagctcgcct tcgacgcggt agccgcagtt cgtgcaggcc
    11641 cagttgaggg gctcggcctc gccctgcacc tcgtgcttat cgtcggtcat tcttgctcct
    11701 cctctgtgct ttcagaggcc attatgacac cagtgaagac ggggatcacg ccgatgttag
    11761 actatccgcg ccgcagccac gaccggaaat gttgtgatca ggttgccgaa cggtcgctct
    11821 ctcgctacac gcttctcgga tcgtttgcgg ggtgcgccac cgacggcgct cgtgatcttc
    11881 cctatattct ccgtccagct cggggccgcc ctggccaaga gctttttcga ctcccttggt
    11941 cccggtggga tggtcttctt gaggatcgcg ttcgccgcac tcgtgctttt cctcctggta
    12001 cgcccgaagt tgggtggata cgaccgggcc gggtacctcg ttgctggcct cttcgggctc
    12061 acgcttgcgg ggatgaacct ctcgctctac ctggccttgg accgcattcc cctgggcgtt
    12121 gccgttacgc tcgagttcgt gggtcctctc ggtgtggccg tcgccggctc ccggcgattg
    12181 ctcgacctgc tatgggtcgt gctcgccgcg gccggcatca tcctgctcgc tcctctcggc
    12241 gtgctcgggg ggatggacct cgaccctgtg ggtgtggcct tcgcgctgct ggcgggatgc
    12301 ctgtgggcct gctacatcct tctctccgtc cgcacgggga gcgctttccc cggcgggacg
    12361 gggcttgtga tcgcgctctg cgtagggacg ctcgtcctgt ttcctttcgg gatagtgggc
    12421 gcaggctacg ccttgctcga cccgaaattc ctcctcgccg gcttcggcgt ggcggtgctc
    12481 tcctcggcca tcccttactc gctggagctc gaagccttgc gcaagctccc cgcccgcgtc
    12541 ttcggcgtcc tgatgagcct cgagccggcc gtggccgcgc tcgccggcct ggtcgtcctg
    12601 ggggaacggc tggagatgcg cgccgtggcg gccattatct tcgtgaccat cgctgccgcc
    12661 ggcgcctcgc tgttcggcag gcgtgacgag ggctgacgtt agcagggtgg cgagatgccc
    12721 cgctgcccgt agaatggaga aaccggtgag tctattggag gagcgaaagg ggatctctgt
    12781 ggcgctggct accggcggtt acttccttgg cggagagctc ctgccccggt gagtgcgcgg
    12841 gtcctcaccc tgagggagct aaaccgggcg acgctcgacc gccagatgct gctcgaccgg
    12901 caatctatcc ccgcccttga cgccgtagag cgcctggccg gcctccaggc ccaggtcccg
    12961 agccctccct atgtcggcct gtggacccgc ctcaaggact tccggcgcga ggagctcacg
    13021 cgcctcatgg aagagaggca ggtagtcagg gcgacgctaa tgcgcgcgac gttgcacctc
    13081 atggcggcgg aggattacct actattgcgt cccgccctgc agccggccct cacgagatcc
    13141 atgaactcca tcgccggaaa acgattggaa ggactggacc tcgaccggat cgtggggatc
    13201 gcgagggagt atttcgagag cgagccgcgt cccttcgccg actttcgtct cctgctcgcc
    13261 gagctcgaac cggagaggga ccaatcggcc ctcgcatacg cggtgaggac ccagcttccg
    13321 ctcgtacagg tgcctacggg tggcgtgtgg ggctactcgg ggaaggtgcc ctttataacc
    13381 gccgagaagt ggctgggcag agatctctct ggttccgagg acccgcgcgg gctcgtgctc
    13441 aaatatctcg cggcgttcgg acccgcgacg gtgagggacg tccagacgtg gtctggcagg
    13501 atacgactca acgagtcggt agaggagatc aaaccggagt tgcgcatctt ccgggacgag
    13561 aatgacaacg agctgctcga ccttcctgac gctccgctgc ctcccgggga catcccggcc
    13621 ccgacccgct tcgtccccga ttacgacaac ctggtgctct cccacgccga cagggggcgt
    13681 gtgatctccg acgagcaccg caagaaggtc ttcctctcgg cggccagggt cagggccacg
    13741 ttcctgatcg acggtttcgt gcgcggcgtg tggaaggtcg agaagaccag gagagcggcg
    13801 acgctcgtca tcgaaccctt cgagcgcctg tccaggggag atcgcgactc cctcagcgaa
    13861 gaaggcgagc gtctcgtccg ctttcttggg gaaccgcagg gcgccgagac gttcgacgtc
    13921 cggttcgtgt agccgacctc gaagggagcc cggggtacga caggtcggga gccacgatgc
    13981 cggaagaaga cgagttcgga aaacggatcg tggcgcgtga taaggtctcg tccttctacg
    14041 gtgtcagaac gatagagagg agccgaagtc cgtgaggttt cacctgatcg ttctggccct
    14101 cgggacgttc gcgatcggga cgggcagctt cgtcttggcc gggccgctcg aaggggtggc
    14161 gagggacctc tcggtctcgg tcgggacggc ggggaatctg gtgacggtct tcgctattac
    14221 ctatgcggta agctcccccg tgctcgttac gcttgtgggg agcgcggcgc cgagacgggt
    14281 tctggttgca gcgatggcgg tattcgtgtt gggtaacgcg gcggcggtgg ccgcgccgac
    14341 cttcggcctg ttggtcctgt gcaggatact cgcggcgtg
//
LOCUS       DATMVF010000032         5782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_555132, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000032 DATMVF010000000
VERSION     DATMVF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555132"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VN178_01265"
     CDS             complement(<1..403)
                     /locus_tag="VN178_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79621.1"
                     /translation="MDKLETATFEIEEERVAWEGLASSCAAPIRRFAAFTLVGFTSFI
                     AITAAVIVFYNVFGDKLIDGQGVSPPRAAFYVTMLLGVAVAIGGYLIWLIRSLMSYRA
                     FSAVLSRGGLDPHRPTAGGLRAYSDEQLLALR"
     gene            558..1388
                     /locus_tag="VN178_01270"
     CDS             558..1388
                     /locus_tag="VN178_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWS79622.1"
                     /translation="MKVYRAGRRIPDGERQGRVLRPILLTLLVVIVLVLVYLIVPFGG
                     QRVVLLGSDARAGEASRSDTIVITKAGSGMLAVPRDTLVHIPGVGEDKINAAFASGGP
                     ELTVETLESLTGLRMNDYVIVHFGGVEEIVDAMGGITLEVDQPIRVGIDGRRVYIPAG
                     TQTLNGTQALAYVRYRGGPTADIGRIGRQQKFLRELARQSTSPAKLPRLPVTASATWR
                     NIDTNMSLLEAARFAVRTRLSGIGDAELYPGTPQYIGGISYWVPDKEAGSRVVSETIE
                     "
     gene            complement(1416..1610)
                     /locus_tag="VN178_01275"
     CDS             complement(1416..1610)
                     /locus_tag="VN178_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79623.1"
                     /translation="MLEQAYLEAGDPQGGSGFRGDEVRWERPRRPIGKTLRTWGYAVA
                     GNAEGADTNGVVFTRMDRHT"
     gene            complement(1701..2369)
                     /locus_tag="VN178_01280"
     CDS             complement(1701..2369)
                     /locus_tag="VN178_01280"
                     /inference="COORDINATES: protein motif:HMM:NF021501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HWS79624.1"
                     /translation="MGEPGRPYVVCPICGHVFRSTYASTYITVGREADLCPKIPGRPS
                     DGARLIRSAVTMCPVCSFAAGEAFDDLDLTFDERCGIEERLKEDGLLKVYRKGQPPWL
                     GFHAAEVCGKERSLTSRELGDLCLRASWVCRKEKERPFESTFQLRAMRHFMRSLQEDD
                     LIGRELSVTTYLVGELNRRLGNHREALNWYVNAGRTTEGDPRVAWLDRLIDKQSKLAR
                     EQAA"
     gene            2520..2717
                     /locus_tag="VN178_01285"
     CDS             2520..2717
                     /locus_tag="VN178_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79625.1"
                     /translation="MEQRKSEAEVALELMRLVLEHAGSRRLSPTRTLALYHLCKEAVA
                     YSPEDRVTADYLEENLKKLLQ"
     gene            2792..3718
                     /locus_tag="VN178_01290"
     CDS             2792..3718
                     /locus_tag="VN178_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79626.1"
                     /translation="MSVIVDGSVETGLEGRGARCLAVDPKDPDKLYVGTSDEGLFRSE
                     DGGSSWERLSGVEHPRVTAVAVSPVDGAVYAGTEPSALFVSRDEGRSWRELEGMRNLP
                     SAPTWSFPPRPWTSHVRAIALSYADPNLVVAGIELGGVVRSTDGGETWQDQRPGAQPD
                     CHSLSAHPEAAELLYEAGGGGFARSADFGDSWESVDEGMGLHYVWGLAADAEDPSLVY
                     ASAASGPYRAHGRGSSNATIYRRIGEEPWQASLEGLDAFPYALCPDPETSGILYAGLG
                     DGTILRGTETGESWEEIARVPPGLEALAAVST"
     gene            3911..4978
                     /locus_tag="VN178_01295"
     CDS             3911..4978
                     /locus_tag="VN178_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWS79627.1"
                     /translation="MRIRSSFVCVAVILALVAVAGCGGSGGGQGGGGGGDGGGADVRP
                     ALVLDIGGLGDQSFNDSAYAGLQRAKKDLDVQTETLESNAATDYVNNITQLADSGYTP
                     VFAVGFLMTDAVNEIAPQYPDTDFAIVDSVVEPKNAVSLVFREQEGSYLAGVVAGLMT
                     QVKTDYTNPDDNVVGFLGGQESDLIAKFQAGYEAGVASVCPNCKVIVQYAGTTPEAFN
                     DPAKGKEISLQQINEGADIIYHASGGTGAGLFDAAKDKGIFAIGVDANQAKLVPDAPI
                     LTSVVKRVDNAVYQTIQDANDGNFPGGEVIDLGLKENGLSLAPFGRFEGDVPQEVKDE
                     VKKAQQGIISGDIKVPDTPQQ"
     gene            4989..>5782
                     /locus_tag="VN178_01300"
     CDS             4989..>5782
                     /locus_tag="VN178_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS79628.1"
                     /translation="MAEGRTGERKAGATREAPVVLEMRNVTKAFGSVRANDDVSITLR
                     RGEILGLLGENGAGKSTLMKILYGLYSPDSGSVFVDGEEARIRDPKDAVSHGIGMVHQ
                     HFTLIPPLTVAENIVLGAEPRKGTSLDLARAIEDTEELSERYGLRVDPRARVTDLSVG
                     QQQRVEILKALYRDARILILDEPTAVLTPQETEDLFSVLGELVADGLSIILITHKLGE
                     LIGVADRITIIRDGKVVDTVDASGTDEGELARLMVGRDVLLRVEKGA"
BASE COUNT         1091 a         1840 c         1876 g          975 t
ORIGIN      
        1 agcgcaaggc gagcagctgc tcgtcagagt atgctctcag cccccccgcg gtcggacgat
       61 gcgggtccag gccgcccctg ctgagtaccg ccgagaaggc cctgtacgac atcaggctcc
      121 tgatcagcca gatcagatac cccccgatgg cgacggcgac gccgaggagc atcgtcacgt
      181 agaacgcggc ccttggtgga gacaccccct gcccgtcgat aagcttatcg ccgaagacgt
      241 tgtagaacac gatgacggcg gccgtgatgg cgataaagga ggtgaagccg accagtgtaa
      301 aagccgcgaa ccttcggatg ggcgcggcgc aagacgaggc caggccctcc cacgccaccc
      361 gctcttcttc gatctcgaac gtcgctgtct ccagcttatc cacgccgtcc agtatactcc
      421 catcccaccg gtcgagaatg ggttagagat cacgccgcca ggaagcctac gcctcgaagc
      481 gccttcgcgg cgagacgccc gcatgtcgac gctgcgacat ctaccttacc gttgttccct
      541 acaatgtagc tatgggcatg aaagtctaca gggcgggccg tcggattccg gacggtgaga
      601 ggcagggtag ggtactcagg ccaatactcc tgaccctgct ggtagtcatc gtcctcgtgc
      661 tcgtctacct gatcgtgccg ttcggcgggc agcgggtggt cttgctgggg agcgacgcgc
      721 gggccgggga ggcctcgcgc tcggacacta tagtaatcac caaggcggga agcggtatgc
      781 tcgccgtgcc gcgagacaca ctcgttcaca tccccggtgt cggggaggac aagatcaacg
      841 ccgcgttcgc cagcggagga ccggagttga ccgtcgagac gctcgagagc ctgaccggcc
      901 tccggatgaa cgactacgtc atcgtgcact tcggcggggt tgaggagata gtcgatgcta
      961 tgggtgggat cacactcgaa gtagaccaac ctataagggt cggcatcgat ggacgcaggg
     1021 tttacatacc ggcaggcacc cagactctga acgggacaca ggcgctcgcc tacgttcgct
     1081 acaggggcgg cccgacggcg gatatcggga ggataggccg ccagcagaag ttcctgcggg
     1141 agctcgcgcg ccaatccacc tcgccagcca aactcccgcg cctcccagtc acagcaagcg
     1201 cgacgtggcg caacatcgac acgaacatga gccttctcga agccgcccgc ttcgccgtcc
     1261 gcacccgact ctcaggcatc ggggacgccg aactctaccc aggcacccca cagtatatag
     1321 gcgggatctc ctactgggtc ccggacaaag aggccgggtc tagggtcgtg tcggagacga
     1381 tcgaatagca ggcttcaggc tacaggcatc aggtttcagg tgtgtctgtc catgcgggtg
     1441 aagaccacgc cgtttgtgtc ggccccctcg gcgttgccgg cgactgcgta gccccaggtg
     1501 cgcagagtct tcccgatagg tcgcctgggt ctctcccagc ggacctcatc gccacgaaac
     1561 cctgatcctc cctgtggatc acccgcctcg aggtaagcct gctccagcaa agcacgagtc
     1621 tcttcgtagt aatcgtcgtc gcgcggcaga tccgcccccg cactcttgct gatgcctgat
     1681 acctgtagcc tgataccttc ttaagccgcc tgctctctgg cgagcttgct ctgcttatcg
     1741 attagacggt cgagccaggc tacgcgggga tctccttcgg tcgtccggcc cgcgttcacg
     1801 taccagttga gggcttcgcg gtggttgccg agacgtcggt tgagctctcc gacgaggtag
     1861 gtggtgacgg agagctcgcg tccgatgagg tcgtcttcct gcagggagcg cataaagtga
     1921 cgcatggccc tcagctggaa ggtgctctcg aaaggtcgtt ccttctcctt gcggcagacc
     1981 caggaggcgc gcaggcacag gtcgccaagc tcgcgtgacg tcaggctccg ctccttcccg
     2041 cagacctcgg ccgcgtggaa tccgagccac ggcggctggc ccttgcggta caccttcagc
     2101 aggccgtcct ccttcaagcg ctcctctata ccgcagcgtt cgtcgaaggt caggtcgagg
     2161 tcgtcgaagg cctcgcccgc cgcgaacgaa cacaccgggc acatcgtcac ggcactgcgg
     2221 atcaaacgcg ccccgtccga tgggcggcca gggatcttgg gacagaggtc ggcttccctg
     2281 ccgacggtga tgtaggtgct cgcgtaagtg gacctgaaca catggccaca gatcggacag
     2341 acgacgtaag gtctgcccgg ttcacccacg ggcatccctc agctctcgcg ggccgccgga
     2401 ccgcgtctct gagtccctaa tggggcctgg atctgggatc aaagcgtcct cgattctacc
     2461 atagcccccc gctccgccgg tataattccc tgtacagatc ggccagaggg aggcactgta
     2521 tggagcagcg caagagcgag gccgaggtcg ccctggagtt gatgcggctc gtcctggaac
     2581 acgccggaag caggcgactc tccccgaccc gtaccctggc cctctaccac ctctgcaagg
     2641 aagccgtcgc gtattccccg gaagaccgcg taaccgcaga ctatctagaa gaaaacctca
     2701 agaagctgct ccagtagcat cagttatcgg ttgcttgtac ataaaaggag gtctcgccgt
     2761 ggcacgattg tacgtggcag gaggaaaggc cgtgtccgtg atagttgacg gtagcgtcga
     2821 gaccgggctg gaagggcgcg gcgcccggtg tttggcggtg gatcccaaag atcccgacaa
     2881 gctctatgtc ggcacctcgg acgagggtct gttccggagc gaggacggcg ggagcagttg
     2941 ggagaggctc tcgggggtcg aacaccccag ggtcaccgcg gtcgccgtct cgccggtcga
     3001 tggggccgtc tacgcgggta cggagccctc ggccctgttt gtcagccggg acgaaggccg
     3061 ttcctggcga gaactggagg gcatgaggaa tcttccctcc gcgcccacct ggagcttccc
     3121 gccccgaccc tggacctccc acgtccgtgc gatcgcactc tcctatgcag acccgaacct
     3181 cgtcgtggcc ggcatagagc ttggcggggt ggtgaggagc accgacggcg gtgaaacctg
     3241 gcaggaccag agacccggtg cccaacccga ctgtcattcc ctgtcggcgc atcccgaagc
     3301 ggccgaactg ctgtatgagg cgggtggcgg cgggttcgcc aggagcgcgg atttcgggga
     3361 cagttgggaa tcggtcgacg agggaatggg cctgcactac gtgtgggggc tcgccgccga
     3421 cgcggaggac ccttcgctcg tctacgcttc agcggcctcc ggaccgtacc gggcgcacgg
     3481 cagagggtcc tctaatgcga ccatctaccg caggataggg gaggagccat ggcaggcgtc
     3541 tctggagggc ctggacgctt ttccctacgc gctctgcccg gacccggaga cgtccgggat
     3601 cttgtacgct ggcctcggag acgggacgat actgcgcggc acggagacgg gggagagttg
     3661 ggaggagatc gcccgcgtgc ctcccggact cgaggcgctg gcggcggtgt cgacgtaggg
     3721 gtcgttcggc ggtttccggg ttgcgcaccc aggtcgcctt catgagagcc ggggacgatc
     3781 ttgggccgtg tctggcggcg caatgcgtta ctctatatga tgctgtgaac gatccactgg
     3841 acgaacaggc aaggagtcgg ccgcccgtcg agactcgaac gaggagcggt cttctgcaaa
     3901 aggaggtccc atgcggataa gatcgagctt cgtctgtgta gccgtgatcc tggctctcgt
     3961 ggccgtggcc ggctgcggcg gctcgggcgg cggtcaggga ggtggcggag gtggcgacgg
     4021 cggtggcgct gacgtacgtc ctgccctcgt gctcgacatc gggggtttgg gggatcagag
     4081 tttcaacgac tcggcctacg ccggtctcca gagggccaag aaggacctcg atgtgcagac
     4141 cgagaccctc gaatcgaacg ccgcgaccga ctacgtgaac aacatcaccc agctcgccga
     4201 cagcggctac acccccgtgt tcgccgtcgg gttcctcatg acggacgcgg tcaatgagat
     4261 cgcgccccag taccctgata ccgacttcgc catcgtggac tcggtagtcg agcccaagaa
     4321 cgcggtaagc ctggtcttca gggagcagga gggcagctac cttgccggtg tggtcgcggg
     4381 gctcatgacc caggtaaaga cggattacac caacccagac gacaatgtcg taggcttcct
     4441 cggtgggcag gaatctgatc tgatcgccaa gttccaggcc ggttacgagg ccggtgtggc
     4501 gtcggtctgc cccaactgca aagtcatcgt gcagtatgcg ggcacgaccc cggaggcgtt
     4561 caacgatccg gccaagggca aggagataag cctccagcag ataaatgagg gcgccgacat
     4621 catctaccac gcctccggag gtacgggcgc tggcctcttc gacgcggcca aggacaaggg
     4681 catcttcgcc atcggcgtgg acgctaacca ggccaagctc gtccccgacg cgcccatcct
     4741 cacctccgtg gtcaaacgcg tagataacgc ggtctaccag accatccagg acgccaacga
     4801 cggcaacttc ccgggcggag aggtgatcga cctcggcctg aaggagaatg gcctcagcct
     4861 cgccccgttc ggacgcttcg aaggcgacgt accgcaggag gtaaaggacg aggtgaagaa
     4921 agcccagcag ggcatcataa gcggcgacat aaaggtcccc gacacaccgc aacagtagga
     4981 accgccagtt ggccgagggg cggacagggg agaggaaggc cggggccact cgggaggccc
     5041 cggtcgtact cgagatgcgt aacgtcacca aggcgttcgg gtctgtaagg gccaacgacg
     5101 acgtctccat cacgctgcgc cggggtgaga tccttggcct cctcggcgag aacggggctg
     5161 gcaagtcgac cctgatgaag atcctctacg gtctgtactc cccggattcc ggctccgtct
     5221 tcgtggacgg cgaggaggca cggatacgcg accccaagga cgccgtatcc cacggcatcg
     5281 ggatggtcca ccagcacttc accctgatac cacccctcac cgtggccgag aacatcgtcc
     5341 tcggcgccga acctcgcaag ggcaccagcc tcgacctcgc cagggccata gaggacacgg
     5401 aggagctctc cgagcgctac ggcttgcggg tcgaccccag ggcgcgggtc accgacctct
     5461 cggtgggaca gcagcagcgg gtggagatcc tcaaggccct ctaccgcgac gcccgcatct
     5521 tgatcctcga cgaaccgacc gccgtgctga cgccccagga gaccgaagat ctgttctccg
     5581 ttctggggga gctggtagcg gacgggctct ccatcatcct catcacccac aagctcggcg
     5641 agctcatagg cgtggccgac cgcatcacca tcatccggga cggcaaggtg gtcgacaccg
     5701 tcgacgcctc ggggacggac gagggcgaat tggcccgcct gatggtcggc cgcgacgtcc
     5761 tgctgcgcgt ggagaaaggg gc
//
LOCUS       DATMVF010000033        18899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_372207, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000033 DATMVF010000000
VERSION     DATMVF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18899
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372207"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VN178_01305"
     CDS             <1..844
                     /locus_tag="VN178_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWS79629.1"
                     /translation="RPSAEGVKVLLDCANGAAYEVAPLMFREIGVDLTLTGADPTGTN
                     INEDCGSMHIEELDASGHDVAFAFDGDADRVLALDEQGNVVDGDRIIAILASDKKKRG
                     VLSGEVVVTVMSNLGFFKAMEAMEIPYEVTDVGDRHVAEAMGKLGTSLGGEQSGHVIL
                     AEHTTTGDGIVTALALLDVMVCSGKPLSELAAAMEVYPQTLINVRVEEPGAAKAVAGT
                     EAVRLAVSEAEKRLGEEGRVLLRPSGTEPVVRVMVEHTDEMVCREVCEEISRAVRDAG
                     KEGT"
     gene            841..1698
                     /locus_tag="VN178_01310"
     CDS             841..1698
                     /locus_tag="VN178_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79630.1"
                     /translation="MRFVGCALAWRPGEARDKVSCLVVLDERGSIIANSFVADAEEIA
                     GTVEDYGSERRGTVVGVDAPLAVPNERGTRKIERILSKVALPAYSASRRMFGGEPYAE
                     ELLSELEKVGVEYTDYPFPRERGQSVVVEVDSAATLKVLSLERSGATDNGDLAKRLRA
                     MADPKLRKGNKESRAVALKGAIEVLWDTPGLRLRTGNLSADISASENVDVSKLDVSAS
                     MSHAELDRTVGLVEGTLAAYTVHRHWRGRDGSIVVGAGDEGSVLLPARNALRERLAEE
                     CSNAGVPYV"
     gene            1836..2639
                     /gene="hisF"
                     /locus_tag="VN178_01315"
     CDS             1836..2639
                     /gene="hisF"
                     /locus_tag="VN178_01315"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWS79631.1"
                     /translation="MSLKVRIIPCLDVDAGRVVKGTKFTDLRDAGDPVELAALYDREG
                     ADEIVFYDITASHEQRDTAAALARAAAEEVFVPYTIGGGVRTAGDMRAMLRAGADKVS
                     INSAAVGNPDLITEGAERFGSQCVVLSVDVKRRGLSPGWEVYLNGGRVNTGMDAVGWL
                     IEGERRGAGEFVLNSMDADGTETGYDLDLISAVAEKTTLPIVASGGAGSPEHMIAAVK
                     AGAGAVLAASIFHFGEYSIAEVKEHMMQAGIPVRLVDQEPDVVSGTGGW"
     gene            2647..3288
                     /gene="hisIE"
                     /locus_tag="VN178_01320"
     CDS             2647..3288
                     /gene="hisIE"
                     /locus_tag="VN178_01320"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HWS79632.1"
                     /translation="MTPAQDRVVFDENGLVPVIAQDVATGDVLTLAYANREAVEMTLS
                     SGEAHYYSRSRSELWRKGATSGNTQRVVGVKLDCDGDALLYRVEPRGPACHTGQNSCF
                     FTTLAGEGVGVAPADDREVPFGTMVERLAGTIARRHREMPEGSYTVSLIQGGKERLAQ
                     KVGEEAVEVVVAALSDERLPEEAADLVYHLLVLLEERGVGIEDVAKVLHDRHG"
     gene            3272..4789
                     /gene="trpE"
                     /locus_tag="VN178_01325"
     CDS             3272..4789
                     /gene="trpE"
                     /locus_tag="VN178_01325"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HWS79633.1"
                     /translation="MTATASLSPVPSLGEARRLARSYDVVPLYAEFIGDLETPISAVL
                     RFAEEDTVFLLESAESAERFGRYSFLGFDPKRTLSYRDGLYTVVDADGVREVPAEDPF
                     RGLAAIVGKKSVAPLPNLPAFVGGAVGYFSYDAVRYLERLPDAPKDDLDVPEACFAVT
                     DTLIVFDHLRHKVLVVSLVDAAGLRDVEGEGFSAAYRRAADDIRRIAERLSAPLVRRT
                     PAFGTGVEISSNFTREGYEEAVAAAKEYIRAGDAFQIVPSQRFAAEIGDLDPLLLYRG
                     LRTVNPSPYMTYLKLGDLSIVGASPEPLVRVEGRRVMTRPVAGTRGRGGTPEEDAALA
                     EELLADEKERAEHVMLVDLGRNDLGRVSEVGSVGLESFMEIERYSHVMHIVSTVEGDL
                     REDLTALDALAAAFPAGTVSGAPKVRAMEIIDELEGTRRGPYAGATGYYGVDGRLDTC
                     ITLRTALLKNGVAYFQAGGGVVADSVPSLEYEETRNKARAMERALEVARTREMWL"
     gene            4924..5523
                     /gene="plsY"
                     /locus_tag="VN178_01330"
     CDS             4924..5523
                     /gene="plsY"
                     /locus_tag="VN178_01330"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HWS79634.1"
                     /translation="MLEILLVLFGYVLGSVPTGYLVGRALGVDVRKIGSGNIGTANVL
                     RAAGKWAAVLTLAGDMLKGLVPVVVARLTVQNEWLIAAVAFAAVIGHCWPVFLRFKGG
                     KAVATGAGTTIGLAPLVGLCLFAFWWAVVLLSRYTSLGAIAVMVISPVVFWLSGQPLP
                     YVLYTVVGGALVLWRHRENARALLQGTERKVGQRAEEEG"
     gene            5524..7779
                     /locus_tag="VN178_01335"
     CDS             5524..7779
                     /locus_tag="VN178_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HWS79635.1"
                     /translation="MLGEAYEGKKILLTGGTGFLGTALVGKILRSLPDLGRLYLLIRP
                     YRGKSAAERFERDVLGAAAFRGLRERLGDDFGERISKKIRVLEGDVHAPSLGLGEEDL
                     TELSREVDVMIHSAASVIFDAPLDAAVDSNVRGTLGLLKLARAWEKKPLFIHISTAYV
                     AGTSKEDAPEAPPGPSSPDGTALDAREEVLGLEAVVAEVDGASQERSLLRRFETEARR
                     ELGMVGEEEEVAERVDQLRRAWMRERLVERGTLRARELGWNDVYTFTKSLAERLVVTE
                     RGETPLVIVRPAIIESSHKEPYPGWIQGTRMADPIIMAFAKGILREFPGNPKSYVDIV
                     PVDHVVNAILAAGVRRPKEPEIFQVASGERNPIRYRDLYDIVRDYFVKYPLRDSSGRP
                     IQVPQWSFPGRRRVEGQLKMELAGLKVAGFLAARMPEGHMASDARQRIARAEKRARMS
                     LYYSRIYGAYANMTTTFASSRTEALYAALPPEDREEFPFDITDIDWRAWMQDAHLPAL
                     TTRPSRKKRKKRADEKPGEVAAIFDVDGTIVGSNVVSYFAWLRMRELPAILRPLWLAG
                     FLTKVPYYWGLDKLSRAHFNRAFYKNYAGWKPSRASYLGRESFAGFTLDRIFPDALQQ
                     LRDHKAAGHRVVLLSGALDFLLEPMKDLADDVLCSTLAQENGAYTGELTGAPVAGEAR
                     ARMLASFARRRGLDLSRSYAYADSISDLPMLEAVGNPVAVNPDRRLGTAAKARGWKVE
                     HWDTDGVAKKI"
     gene            7781..8983
                     /locus_tag="VN178_01340"
     CDS             7781..8983
                     /locus_tag="VN178_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWS79636.1"
                     /translation="MQALVYRKSIPRYLLMRAGSRRIKTLETSRFSPLQLEDAPDPVL
                     PAPQWVRVRPLLSGICGSDLGTLSSENSPYFSPITSPPFVMGHEVVGEVAEDNSGFRA
                     GERVVLEPALGCAVRGIDPPCPYCASGRYALCLNVARGDISPGIQTGFCRDTGGGWSR
                     GTLVAHPSQLHRVPDAVPDEAAVAIEPLACAVHAALGTSPGETTLVIGAGSVGLLTVA
                     ALRHLTDAGRIICVAKHERQKSEALRLGATEVVHPKETYSNLPEMLGTTPYKPELGKP
                     VVMGGAGKVFECVGAPGTLEDALRLTKPGGDVTLVGMPGAKSTLDLTALWYKEVRLAG
                     SYAYGAEEHDGKQSSSFQLALSIAPEIGLQTLIGPSFRIGSYKEAIAAARSAGSEGHV
                     KVVFDHRT"
     gene            9130..9729
                     /locus_tag="VN178_01345"
     CDS             9130..9729
                     /locus_tag="VN178_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HWS79637.1"
                     /translation="MAEGRAEIAGYEFGTRGVVRSPLGLEDLDLLKQTVLFGEEDEEH
                     LRMAGDVLEDQVEDVLDVWYGFVGDHPHLLRYFSDSEGRPDTEYLGRVRGRFGQWILD
                     TCRRPYDQEWLDYQQEIALRHTRDKKNQTDEAYGTPDHIGLRYMVAFIYPITATIKPF
                     LANKGHSAEDVDKMHQAWFKSVVLQVTLWSYPYVKNGDF"
     gene            9719..10351
                     /locus_tag="VN178_01350"
     CDS             9719..10351
                     /locus_tag="VN178_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWS79638.1"
                     /translation="MTSRLGPVFDLSAQHDDVDAKVVAALERLSQVFRARLREEAWEH
                     GLSPTQAQFMVYLLYHDVELRRISQLAREFDLTQATVSDAVASLETKKLIQRERWPED
                     RRIVTLRLTPDGEKLATILSGWADPIREHLRPFSPEDREAVMRFLIGLIGSLQRYGLI
                     TVARMCVTCKFFQQDIHPGEPLQHHCALLDVPLGRANLRVDCPEHEPAVG"
     gene            10565..12139
                     /locus_tag="VN178_01355"
     CDS             10565..12139
                     /locus_tag="VN178_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWS79639.1"
                     /translation="MRPGITTILEKNSPPMWFYAGDRFHYEKLPEGTRVIYPPGPSEP
                     LAEPGVAIERALLEPIGMEPLHELLTPGMKLTIAFDDLSIPLPPMRKPDIRQLVIEKV
                     LEKAAARGVEDIHLIAALGLHRRMTEAELRHCLGSRIMSRYYPERLYNYDAEDPDGNV
                     RLDETDQGEEVSVSRRVAESDLLVYVNVNFIPMDGGHKSVHTGLAPYSSIRHHHTPKT
                     LANTRSLMDPPNSAMHASINRMGRIFDEHVKVFHIESTLNNQTYPAAFDFMAKPEYQW
                     NMLTRANFLATHGATRTMPLEAARRISHSIKAPHRVTSVQAGATEPVHAETLKDNYRQ
                     MVVPAEGQADILLLGIPYLGPYNVNSIMNPILVFNMLLGYLFNLYRGKPLVREGGVLI
                     GTHPMPANFDKLHHPSYIDLWNEVFPETNDVYEIHHRYEEKYANDPWYRTLYRNSYAY
                     HGAHPFTVLYWAAHALDHLGGVILVGGDPENTKRMGLGRADTVSDALQMAEDTVGPNP
                     STTYMHIPPLFMCEVS"
     gene            12223..12666
                     /locus_tag="VN178_01360"
     CDS             12223..12666
                     /locus_tag="VN178_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWS79640.1"
                     /translation="MPTRARGLRLPEELQWEIDREMRLRGETFSEVATSLLREAVRMR
                     RVPGIVFMDGPVGRRASIAGTGLDVWEVIATFKSVGEDRGRLEASYGWLSERQLSAAL
                     AYYSLYPEEIDARIEEEESWTPEKLYREFPYLRPRSVQSSDKPEA"
     gene            12663..13010
                     /locus_tag="VN178_01365"
     CDS             12663..13010
                     /locus_tag="VN178_01365"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWS79641.1"
                     /translation="MRFLLDEDLPPRAAEIARGLGLDVISVHELGRQGISDRDQLRFA
                     SEDERIFLTRNHRDFIELTVEFYRAGEAHSGVLLVRRNLPNDRPARVAHAMKRWADAR
                     ADTLATFDGVDFL"
     gene            complement(13014..13727)
                     /locus_tag="VN178_01370"
     CDS             complement(13014..13727)
                     /locus_tag="VN178_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79642.1"
                     /translation="MQILVNHLTRMQPGYFCVAGIDVDTPRHIRPVLGHGRRLTIDLL
                     STGGGPFDAGSVVDLGPATYAGHAPELEDHRFEPSRARRLFDDDPDDYWGAIDGSAKE
                     SLEGIFGPDLELWDESGTVDVGKGRASLGCLKPEEQPWLYMDHRGTVRLVLDYLMPSV
                     DLAVNDLRLYERDGRTPRRDLVASVEKRLKSGVEVILSVGLTRPWQKRGDTAERHWLQ
                     INNIHFKDDPLWQLRDRPS"
     gene            complement(13735..14112)
                     /locus_tag="VN178_01375"
     CDS             complement(13735..14112)
                     /locus_tag="VN178_01375"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWS79643.1"
                     /translation="MNDAGIERLLDVRLNNASRLAGFARRADLPFFLQRLCGAEYVYE
                     PRLAPTREILDAYRKGGGWPDYERAFLDLLRERRVEDTLDRSLFEVPTVLLCSEASAE
                     HCHRRLVLEYLGRAWKDFRAVHL"
     gene            complement(14109..14666)
                     /locus_tag="VN178_01380"
     CDS             complement(14109..14666)
                     /locus_tag="VN178_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWS79644.1"
                     /translation="MFTVGHSNHSAEKFAGLLRRHGIEAVVDTRSHPYSRHAPQFNAC
                     AIEAVLSKVGIGYLFLGRELGGRPAGIEFYDAKGRVDYTQVGCSRPFLDGISRLEQEV
                     STRTVVLLCSEEDPARCHRRLLVGRALEERGFVLRHIRGDGSIHIEGEMDGGQPVLFP
                     ETEVSPRKSIRSVSRRRRRPSSSER"
     gene            15278..16498
                     /locus_tag="VN178_01385"
     CDS             15278..16498
                     /locus_tag="VN178_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79645.1"
                     /translation="MKSLRVNGRLSFIVLLISQLAATMGFTFVLPFMPIYVQELGVED
                     PGSAAAWAGVINSSTAVTMALAAPLWGRLGDRVGLKPMLLRATIAGSLLLGLMGLVGS
                     PWQLLALKTLQGCVTGTVAAATVLVSATAPPNRAGERLGTLQMVIFVAAAAGPFFGGA
                     FADLVGIRASFGVTAALLAASAVLISFWVKEERPEEEEVEGEESSDAEGPLPHRRLAP
                     TLLALFVVQVAIMSAAPALPGFLSTLMEEPVRVATLAGWIIATGALAASLGSVIGGKL
                     AARFGARKVIIWTLLLAGISALPQAEVDSVALLWVLRLVTGLFVGIAVPVANLAIRGA
                     VHPRRQGEAFGVAATATSAGNAVGPAAGGLLASSFGFGAPFLVPGLCLAAVSAIIWLA
                     PLLAVGYRTAVRDL"
     gene            complement(16661..17368)
                     /locus_tag="VN178_01390"
     CDS             complement(16661..17368)
                     /locus_tag="VN178_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWS79646.1"
                     /translation="MAQPIDLYYWPTPNGWKITIFLEEAGLPYNLVPVDITSGDQYES
                     EFLKISPNNKIPAIVDPEGPDGEPMSLSESGAILIYLADKTGEFLPEAPRDRYLVLQW
                     LMFQMGHIGPMLGQAHHFRGYAPEKIPYAIERYTDEAARLYRVVDQRLSEAEYFAGEE
                     YTIADMAIYPWLVSHEKQGQVLEDYPDLRRWYEAMESRPAVSRALEVGKELHRSLDEM
                     DDKTRKTLFGGGPEGRR"
     gene            complement(17517..17717)
                     /locus_tag="VN178_01395"
     CDS             complement(17517..17717)
                     /locus_tag="VN178_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79647.1"
                     /translation="MRETDTQKARARERNLRWMEALWEGTSTSAKEEDQKEEVLAMFR
                     DLARESRSGRAVREYVPWQPLW"
     gene            17972..18130
                     /locus_tag="VN178_01400"
     CDS             17972..18130
                     /locus_tag="VN178_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HWS79648.1"
                     /translation="MDLLRIIIAILLPPLGVFLQVGIGKQFWINILLTLLGYIPGIVH
                     AVWVIAKY"
     gene            complement(18144..>18899)
                     /locus_tag="VN178_01405"
     CDS             complement(18144..>18899)
                     /locus_tag="VN178_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79649.1"
                     /translation="RRAEMIGVMVSAAVVGALLGPVLGSVAATVGLLPAFASVAIAGG
                     VIAVWAALERAPASSGDVSLLPALRTVREPLLLASLWFIVLSPLLFSALAVLVPLDLN
                     RLGWGAAAVGTVFFVSAIFEAFVHPLLGRWSDRSGYRPPVFAGLLASIGILLLLTWAG
                     NPWMIGLLVVLAAGAFNATLVPGTALFSRGTEKAGMERAVAFAITNFAWASGYAVGAP
                     LGGFLADLRGDALSYLALTAVCLSTIVLIRRTA"
BASE COUNT         3527 a         6048 c         6046 g         3278 t
ORIGIN      
        1 gcgtccgtcg gctgagggtg tcaaggtgct cctggactgc gccaacggag ccgcctacga
       61 ggtggcgccg ctgatgttcc gggagatcgg agtggatctc accctgaccg gagccgatcc
      121 cacgggtacg aacataaacg aggactgcgg ctcgatgcac atcgaggaac tcgacgcctc
      181 agggcacgac gtggctttcg ccttcgacgg ggatgcggac agggtgctcg cccttgacga
      241 gcaggggaac gtcgtcgacg gggacaggat catcgccatc ctcgccagcg acaagaagaa
      301 acgcggagtc ctctcaggcg aggtcgtggt cacggtcatg agcaacctcg gcttcttcaa
      361 ggccatggag gctatggaga tcccttacga ggtgactgat gttggggaca ggcacgtcgc
      421 tgaggctatg ggcaagctcg ggacttccct gggcggagag caatcggggc atgtgatcct
      481 ggccgaacac accaccacgg gagacggcat cgtcacggcc ctcgcccttc tcgatgtgat
      541 ggtttgcagc ggcaagccgc tctccgagtt ggccgcggcg atggaggtgt atccgcagac
      601 gctcatcaac gtgagggtcg aggagcctgg ggccgcaaag gccgtggcag gcacggaggc
      661 ggtacggctg gccgtctcag aggcggagaa gaggctcggc gaggaaggac gcgtcctcct
      721 caggccgagc ggaacggagc cggtcgtgcg ggttatggtc gagcatacgg atgagatggt
      781 gtgccgggag gtctgcgagg agatctctcg cgccgtacgc gacgcgggta aggagggaac
      841 ttgaggtttg taggctgcgc gctggcgtgg aggccgggcg aggctcggga taaggtctcg
      901 tgcctggtcg tgctggacga gcgggggagc atcatagcca actcgttcgt ggcggacgct
      961 gaggagatag cgggaacggt cgaggattac ggctcggagc ggcgggggac cgtcgtaggc
     1021 gtcgacgcgc cgctcgctgt ccccaacgag aggggaacgc gcaagatcga acgcatcctc
     1081 tccaaggtgg cgctccccgc ctactcggca agccgcagga tgttcggcgg cgagccttac
     1141 gcagaggaac tgctctccga actggagaag gtcggcgtgg agtacacgga ctaccctttc
     1201 ccgagggaga ggggccagag cgtggtcgtg gaggtagatt ccgcggcgac gctgaaggtc
     1261 ctctccctgg agcgctcggg tgcgacggat aacggcgacc tcgcaaagcg cctccgggcg
     1321 atggccgacc ccaaactccg aaagggcaac aaggagagca gggcggtggc gctcaagggt
     1381 gctatcgaag tcctctggga cactcctggc ctcaggctaa ggacgggcaa cctctctgcg
     1441 gatatctccg cctcggagaa cgtagacgtc tccaaactcg acgtctccgc ctcgatgtcc
     1501 cacgcagagc tcgacaggac ggtcggcctg gtggaaggga cgctcgccgc ctatacggtg
     1561 caccgtcact ggagggggag ggacggctcg atcgtggtgg gcgcggggga cgaaggctcc
     1621 gtcctgctgc cggcgaggaa cgccctgcgc gagcgtctgg ccgaagaatg ctccaacgcc
     1681 ggtgtgccct acgtatagcc ccgtacaata ccccgtaatg agtgtcagga atgaacagtc
     1741 gtgaagccca tttcgtcgaa cgtgaccctg ccacaggagc agcgtcacgc acgcatccag
     1801 cgcccggctc gacctgggcg aaagaagaga ctctggtgag cctcaaggta cgcatcatac
     1861 cctgcctcga cgtggacgcc gggcgggtcg taaaggggac gaaattcacc gacctgaggg
     1921 acgcaggaga ccctgtcgag ctcgcggccc tctacgacag ggagggggcg gatgagatcg
     1981 tcttctacga catcacagcc tcccacgaac agagggacac ggccgccgcg ctcgcccgcg
     2041 ccgcagcaga ggaggtcttc gtaccgtaca ccatcggcgg cggcgtaagg acggccggcg
     2101 acatgcgcgc catgctgcgc gcgggggccg acaaggtctc cataaacagc gccgccgtgg
     2161 ggaacccgga tctcatcacc gaaggagcgg aacgcttcgg gagccagtgc gtcgtactga
     2221 gcgtcgacgt gaagcggcgc ggcctctccc cgggctggga ggtctacctg aacggcgggc
     2281 gggtcaacac ggggatggac gccgtcgggt ggctcatcga gggagagagg cggggggcgg
     2341 gggagttcgt gctgaacagc atggacgcgg acgggaccga gaccggttac gacctcgacc
     2401 tgatctccgc cgtcgccgag aagacgaccc tccccatagt cgcgtccggc ggggcgggga
     2461 gtccggagca catgatcgcg gccgtcaaag ccggagctgg cgcggtgctg gccgcgagca
     2521 tcttccactt cggggagtac agcatagccg aggtcaagga gcacatgatg caggccggca
     2581 tcccggtccg gctcgtcgac caggagccgg acgtggttag cggcacaggt ggatggtgat
     2641 agaaggatga ccccagcgca ggatagagtg gtgttcgacg agaacggtct cgtgcctgtt
     2701 atcgcccagg atgtggcgac gggcgatgtg cttacgctgg cctacgccaa ccgcgaggcc
     2761 gtcgagatga cgctctccag cggcgaggcc cactactact cccgctcgcg ctccgagctc
     2821 tggcgcaagg gcgcgacgag cggcaacacc cagagggtcg tcggggtcaa gctggactgc
     2881 gacggggacg cgctcctcta cagggtcgag ccgcgcggac cggcctgcca tacgggccag
     2941 aacagctgct tcttcaccac cctggcaggg gaaggggtcg gggtagcgcc cgcggacgac
     3001 agggaagtcc ctttcgggac gatggtcgag cggctggcag gcacgatagc gcggcgccac
     3061 agggagatgc cagaaggctc gtatacggtt tccctgatcc agggcggaaa ggagcggctt
     3121 gcccagaagg tgggggagga ggcggtggag gtcgtggtgg cagcgctctc cgatgagagg
     3181 ctacctgagg aggctgccga cctcgtctac caccttctgg tgttgctcga ggaacggggc
     3241 gtggggatag aagacgtggc gaaggtactg catgaccgcc acggctagcc tcagcccggt
     3301 cccttcgctg ggcgaagccc gcagactcgc ccgttcctac gacgtcgtgc cgctctacgc
     3361 cgagttcatc ggggacctgg agacgccgat ctcggcggtt ctcaggttcg ctgaagaaga
     3421 cacggtattc ttgctggaga gcgcggagtc ggccgagagg ttcggccgct actccttcct
     3481 aggcttcgac ccaaaacgca cgctctccta tagggacggt ctctacacgg tcgtcgacgc
     3541 ggacggtgtg cgggaggttc cagcagagga tcctttcagg ggcttggccg cgatcgtggg
     3601 caagaagagc gtagcgccgc ttcccaacct accggcgttc gtgggcggcg ccgtgggata
     3661 cttctcctac gacgccgtgc gctatctcga acgcctcccc gacgccccga aagacgacct
     3721 cgacgtcccc gaggcctgct tcgccgttac cgacaccctg atcgtcttcg accacctcag
     3781 gcacaaggtg ctcgtcgtct cgttggtcga cgccgcgggc ctgcgcgacg tggagggcga
     3841 gggtttctcg gccgcctaca ggcgggcggc cgacgacata cgcaggatcg ccgagcgcct
     3901 ctccgccccg ctggtgcgcc ggacgcccgc cttcggcacc ggggtcgaga tctcctcgaa
     3961 cttcaccaga gagggctatg aggaggccgt agcggcggcc aaagagtaca tcagggcggg
     4021 ggacgccttc cagatcgtcc cttcccagag gttcgccgcc gagatcgggg acctcgatcc
     4081 cctccttctc taccgtggtc tgcgcacggt aaacccatca ccgtatatga cctacctcaa
     4141 gctcggcgac ctctccatcg tcggtgcatc ccccgagccc ctggtgcggg tggagggaag
     4201 gcgcgtaatg acccgtccag tggcgggcac ccgcgggcgt ggcggcacgc cggaggaaga
     4261 tgccgccctg gccgaggaac tactggcgga cgagaaggaa cgggccgagc acgtcatgct
     4321 cgtcgacctc gggcgcaacg acctggggcg tgtcagcgag gtcggctcgg tcgggctgga
     4381 gagcttcatg gagatagagc gctactcgca cgtcatgcac atcgtctcta ccgtcgaggg
     4441 tgatctacgc gaggacctga cggccctcga cgccctggcg gcggccttcc cagccgggac
     4501 cgtctccggc gcgccgaagg tcagggccat ggagatcatc gacgagctcg agggcacccg
     4561 ccgcggaccc tacgccggcg ccacgggcta ctacggcgtc gacggccgcc tcgacacctg
     4621 cataactctc cgcacagcac tcctcaagaa cggcgtcgcc tacttccagg ctgggggcgg
     4681 cgtcgtcgcc gactccgtcc cttcgctcga gtacgaagag acccgtaaca aggcgcgggc
     4741 gatggagcgg gccctcgagg tcgcccgcac ccgcgagatg tggctgtagc gacacgccgc
     4801 aaccggcgtg tcgcgccatc agcactcagc tttcagcagt cagcttccag ttcgatttgg
     4861 gtccgcctag tgcacacgcg tagtgaggcc acggggccat cgttcagcta tactttctgc
     4921 gccatgttag agattcttct cgtgttgttc ggctatgttc tcggctccgt gccgaccgga
     4981 tacctggtcg gacgggcgct cggcgtcgac gtccggaaga tcggcagcgg gaacatcggt
     5041 acggcgaacg tgctgcgggc ggcggggaag tgggccgccg tactcacgct cgcaggggac
     5101 atgctcaagg ggctcgttcc cgtggtggtg gccaggctca ccgtgcagaa cgaatggctc
     5161 atcgccgcgg tcgctttcgc cgcggtgatc gggcactgct ggcccgtctt cttgcgtttc
     5221 aagggcggaa aggccgtggc cacgggcgcc ggtacgacga tcgggctcgc gcccttggtc
     5281 gggctctgcc tcttcgcctt ctggtgggct gtcgtgctcc tcagccgcta cacttcgcta
     5341 ggcgcgatcg ccgtcatggt aataagcccc gtggtgttct ggctctcggg ccagccgctt
     5401 ccttacgtac tctacaccgt agtcgggggc gcgctcgtcc tgtggaggca cagggagaac
     5461 gctagagccc tcctgcaggg caccgagagg aaggttggcc agcgggcaga ggaggagggc
     5521 tagttgctcg gcgaggcgta cgagggcaag aagatactgc ttacaggggg gaccggcttt
     5581 ctggggacgg cgctggtcgg gaagatccta cgctccctcc ccgatctcgg gcgcctctac
     5641 ctcctcatca gaccctatcg tggcaagagt gccgcagaga ggttcgagag ggacgtcctc
     5701 ggcgccgcgg ccttccgcgg cctgcgcgag aggctgggcg acgacttcgg ggaacgcatc
     5761 tcgaagaaga ttcgcgtcct ggagggagac gtgcacgcgc cttcgctagg gcttggcgag
     5821 gaagacctca cagagctctc gcgcgaggtg gacgtcatga tccactccgc cgcttcggtt
     5881 attttcgatg cgccgctcga cgccgcggtc gactcgaacg tacgcggcac cctaggactc
     5941 ctgaagctcg cccgcgcctg ggagaagaag cctcttttca ttcacatctc cacggcctac
     6001 gtggcgggaa ccagcaagga ggacgcgccc gaggcgccac ctggcccgtc ttcgcccgac
     6061 gggacggccc tcgatgcgcg cgaggaggtt ctcggcctcg aggcggtcgt ggccgaggtg
     6121 gatggagctt cgcaggagcg ttcacttctg aggcgcttcg agaccgaggc gcggcgggag
     6181 ctcgggatgg tcggtgaaga ggaagaggtc gcagagaggg tcgaccagct gaggcgggcc
     6241 tggatgcgcg agcggctcgt cgagcggggg accttgcggg cgcgggagct cgggtggaac
     6301 gacgtctata cctttaccaa gtcgctcgcg gagcggttgg tggtcacgga acgtggcgag
     6361 acgccgctcg tcattgtgag gcccgccatc atagagagca gccacaagga gccctacccg
     6421 ggttggatcc agggcaccag gatggccgac cccataatca tggcgttcgc caagggcatc
     6481 ctgcgcgagt tcccgggcaa cccgaagagc tacgtggaca tcgtccccgt agaccacgtc
     6541 gtaaacgcga tcctggccgc cggggtccgc cgccctaaag agcccgagat cttccaggtc
     6601 gcctcggggg aacgcaatcc gatccgctac cgcgacctct acgacatagt gcgcgactac
     6661 ttcgtaaagt accccttgcg cgactccagc gggcgtccga tccaggtgcc acagtggtcc
     6721 ttcccaggcc gcagacgagt ggagggacag ctcaaaatgg agctcgccgg cctcaaggtg
     6781 gccggctttc tcgccgcacg gatgccggaa ggccatatgg cctccgacgc gcgccagagg
     6841 atcgcgcgcg ccgagaagcg cgccagaatg agcctctact acagccgcat ctacggcgcc
     6901 tacgcgaaca tgaccacgac cttcgcgagt tcccgcacgg aagccctcta cgcagccctt
     6961 ccgccagagg accgcgagga attccctttc gatatcacgg acatcgactg gcgggcgtgg
     7021 atgcaggatg cgcacctgcc cgcgctcacg acccgcccga gccgcaagaa gcgcaagaag
     7081 agggccgacg agaagcctgg cgaggtagcg gcgatcttcg acgtcgacgg caccatcgtc
     7141 ggctccaacg tggtctctta cttcgcttgg ctcaggatga gggagcttcc tgcgatcttg
     7201 cgtccgctgt ggctcgccgg attcctgacc aaggtcccct actactgggg actggataaa
     7261 ctcagccgcg cccacttcaa ccgcgccttc tacaagaact acgcgggctg gaagccatcc
     7321 cgcgccagct acctaggacg ggagagcttc gccggcttca ccctggaccg catcttcccc
     7381 gacgcgttgc agcaactaag agaccacaag gccgccggcc accgcgtcgt gctgctctcc
     7441 ggcgcgctcg acttcctcct cgagcccatg aaggacctcg ccgacgatgt gctctgctcc
     7501 acgctggccc aggagaacgg cgcctacacc ggagagctga cgggcgcccc cgtggccggt
     7561 gaggcccgcg ccaggatgct ggcctccttc gccaggcgac gtgggctgga cctctctcgc
     7621 tcctacgcct acgccgactc catctccgac ctacccatgc tcgaggccgt cggcaacccc
     7681 gtcgccgtca acccggatcg gcgcctcggg acggcggcca aagccagggg atggaaggtc
     7741 gagcactggg acacagacgg cgtcgccaaa aagatctagt ttgcaagccc tagtctaccg
     7801 aaagtccatc ccacgttacc tcttgatgcg cgcgggctcg cggcggataa agaccctcga
     7861 aacgagccgc ttctccccgc tccaactgga agacgccccc gaccccgtac tcccggctcc
     7921 gcaatgggtg cgcgtaaggc cgctcctctc cggtatctgc ggctcggatc tgggaacgct
     7981 ctcttcggag aactcgcctt acttctcccc gatcacctcc cctcctttcg tgatgggcca
     8041 cgaggtcgtc ggtgaggtag ccgaggacaa ctccggcttc cgagccggcg agcgggtcgt
     8101 cctcgagccg gccctcggat gcgcggtccg cggcatagat ccgccctgcc cctactgcgc
     8161 ctcgggccga tacgccctgt gcctgaacgt ggccaggggg gacataagcc cgggaatcca
     8221 gacgggcttc tgccgcgaca ccggcggtgg ctggtcacgg ggcacgctcg tcgcccaccc
     8281 ctcgcagctc caccgcgtac ccgacgccgt acccgacgag gccgccgtag caatcgagcc
     8341 cctcgcctgc gccgttcacg ccgcgctcgg gacctccccc ggcgaaacca ccctggtcat
     8401 cggcgccggg agcgtcggcc tccttaccgt cgcggccctg agacacctga ccgacgcagg
     8461 ccgcatcatc tgcgtcgcca agcacgagcg acagaagagc gaggccctcc gtctgggggc
     8521 aaccgaagtc gttcacccca aagagaccta ctcgaacctc cccgagatgc ttggcacaac
     8581 gccatacaag cccgaactcg gcaagcccgt cgtcatgggc ggagcaggca aggtcttcga
     8641 gtgcgtcggc gcgcccggca ccttggagga tgcgctccgc ctcacaaagc ccggcggcga
     8701 cgtcacgctc gtcggtatgc ccggcgcgaa gagcacactc gacctgaccg cgctctggta
     8761 caaagaagtc cgcctcgccg gcagctacgc ctacggcgcc gaggagcacg atggaaaaca
     8821 gtcgagcagc ttccaactcg cgctcagcat cgccccagag ataggcttac agacgctcat
     8881 aggtcccagc ttccgaatcg gatcctataa agaggctata gcagccgccc gctcagcagg
     8941 aagcgaagga cacgtcaaag tggtcttcga ccacaggact tgaccccgat gtaaccacca
     9001 atagcgccac ggtgggccca tctccgcaat ccgacccgcc aggtattgac acatatcttg
     9061 catttgttta gtatattagg agtcctaaca tactggagat tgggtacggg cgtacaggag
     9121 ggagcgaaga tggctgaggg acgagcagag atagcggggt acgagttcgg gacgcggggt
     9181 gtggtccggt cgccgctggg gctcgaggac ctggatctgt tgaagcagac ggtcttgttt
     9241 ggcgaggagg acgaggagca tttgcggatg gcgggggatg tgctcgagga ccaggtcgag
     9301 gacgtgctcg acgtgtggta cggctttgtg ggggatcatc cgcacctgct tcgttacttc
     9361 tcagattcgg agggccggcc ggacacggag tatctcgggc gggtgcgagg gcggttcggg
     9421 cagtggatac tcgacacttg caggcgtccg tacgaccagg agtggctcga ctaccagcag
     9481 gagatagcgc tgcgccacac cagggacaag aagaaccaga ccgatgaagc atacgggacg
     9541 ccggatcaca tcggtctgcg ttacatggtc gcgttcatct atccgatcac ggccaccata
     9601 aagccgttcc tggccaataa ggggcacagc gcagaggacg tagacaagat gcaccaggcg
     9661 tggttcaagt ccgtggtcct tcaggtgacg ttgtggagct atccgtacgt gaagaacggt
     9721 gacttctagg ttggggccgg tcttcgacct ctccgcgcag cacgacgatg tggacgccaa
     9781 ggtcgtcgcc gccctagaaa ggctctcgca ggtctttcgc gcgcgactcc gagaagaagc
     9841 gtgggagcat ggtctcagtc ccacgcaggc ccagttcatg gtctacctgc tctaccacga
     9901 cgtggagctg aggcggatca gccagctcgc cagggagttc gatctcaccc aggccacggt
     9961 cagcgacgcc gtcgcatcgc tggagaccaa gaagttgatc cagcgcgagc gatggcccga
    10021 agaccggcgt atcgtgaccc tgcgtctgac ccctgacgga gagaagctgg cgaccattct
    10081 atctgggtgg gcggatccca taagagaaca cctgcggcct ttctcacccg aggacagaga
    10141 ggccgtgatg cgcttcctga taggactcat aggttccctg cagaggtacg gtctcatcac
    10201 cgtcgccaga atgtgcgtga cctgcaagtt cttccagcaa gacatccatc ccggcgaacc
    10261 gctgcagcat cactgcgcgc tcctggacgt accactaggc cgcgcgaatc tccgtgtcga
    10321 ctgtccggag cacgaacctg ccgtaggctg agttagcatt tcagcattca gcacgcgaaa
    10381 gctaacgcct tcgcttgtca gcccggcggc tttgccgcct tgtcagccag ggattgcgca
    10441 tgcgaaagcc gcactgctga tatgctgaca agcgcccgac agggcgcgtg ctgaaatgct
    10501 gacaagccgc ctggcgaagc ctggcggcga gctgaagtgc tagaagcgac cgaagggagc
    10561 gcctatgaga cccggcatta cgaccatcct ggagaagaac agtcccccca tgtggttcta
    10621 cgcgggtgac agattccact acgagaagct gcccgagggg acgcgtgtga tctaccctcc
    10681 cggaccttcg gaaccgctcg ccgagccagg cgtcgccata gaacgcgcgc tactcgaacc
    10741 gataggtatg gaaccgctgc acgaactctt gacgcctgga atgaagttga ccatcgcctt
    10801 cgacgacctc tcgataccgc tgccgccgat gcgcaaaccc gatattcgcc agctggtcat
    10861 cgaaaaggtg ctcgagaagg ccgccgccag aggcgtcgag gacatccacc tcatcgcggc
    10921 cctcggcctc caccgcagga tgacggaagc cgagctccgc cattgcctgg ggagcaggat
    10981 catgtcccgc tactacccgg agcggctcta caattacgac gccgaggacc ccgacgggaa
    11041 cgtacgtcta gacgagacgg atcagggcga agaggtcagc gtcagccgcc gcgtcgccga
    11101 gagcgacctg ctcgtctacg tgaatgtgaa cttcatcccc atggacgggg gacacaagtc
    11161 cgtgcacacg ggccttgccc cctactcctc cattcgccat caccacaccc cgaagacgct
    11221 ggccaacacc cgctccctga tggacccacc gaactccgcc atgcacgcct ccataaaccg
    11281 gatgggtaga atcttcgacg agcacgtcaa ggtcttccac atagagagta ccttgaacaa
    11341 ccagacctac cccgcagcct tcgacttcat ggcgaaaccc gagtaccagt ggaacatgct
    11401 cacaagagcc aactttctcg ccacccacgg ggcaacgcgg acgatgccgt tggaagccgc
    11461 caggcgcatc tctcactcca taaaggctcc acaccgggtg accagcgtac aggcgggtgc
    11521 caccgagccc gtccacgccg agaccctgaa agacaattac aggcagatgg tcgtccctgc
    11581 cgagggccag gctgacatac tcctgttggg cataccgtac ctcggaccct acaacgtcaa
    11641 ctccatcatg aaccctatcc tcgtcttcaa catgctcctc ggctacctct tcaacctcta
    11701 caggggcaaa ccactcgtgc gcgagggagg agtcctgatc ggcacccacc ccatgccggc
    11761 caacttcgac aagctccacc acccgagcta catagacttg tggaacgagg tcttccccga
    11821 gacgaacgac gtgtacgaga tccatcaccg ctacgaggag aagtacgcca acgatccctg
    11881 gtaccgtacc ctctaccgaa actcctacgc ataccacggc gcccacccat tcacggtcct
    11941 ctactgggca gcccacgccc tcgaccacct cggcggtgtc atactcgtag gcggcgatcc
    12001 cgagaacacc aaacgcatgg gactcggacg cgccgacacc gtatccgatg cgctccagat
    12061 ggccgaggac accgtaggcc cgaacccaag tacgacctac atgcacatcc cacccctctt
    12121 catgtgcgaa gtgagctgaa gaaccatagg acacatcccg atgatgttat acagtgtgca
    12181 caatgtgaac aagacgtcca tactgttgga aagaggggtg cgatgccgac cagggcgcgg
    12241 gggctccggt tgccggagga actacagtgg gagatagatc gcgagatgag gctacggggg
    12301 gagacgttct ccgaggtcgc cacttctctg ttgcgagagg ctgtgaggat gcgccgggtt
    12361 cctgggatag tgttcatgga tggccctgtg gggcgaaggg cgtcgattgc ggggacgggg
    12421 cttgacgttt gggaggtaat cgccactttc aagagtgtgg gggaggatcg tgggcggctc
    12481 gaggcgtcct acggatggct ttcggaacgg cagctctccg cggcgctcgc ttactacagt
    12541 ctctacccgg aggagataga cgcccgcatc gaagaagagg agtcctggac gcccgagaag
    12601 ctatacaggg agtttccgta cctcagaccg cgtagcgttc agtcttcgga caaacccgag
    12661 gcttgaggtt tctcctggac gaggacctgc cgccaagggc ggccgagata gcgcgtggct
    12721 tgggcctgga cgtgatcagt gtgcacgagt tgggccggca aggcatctcg gatcgcgacc
    12781 agctccgctt cgcgtctgag gatgagcgca tcttcctcac ccgcaatcac agggacttta
    12841 tcgagcttac cgtcgagttc taccgggcgg gagaggcgca ttcaggtgtg ctactcgttc
    12901 gccgcaacct cccaaacgac cgaccagcga gggtcgcgca cgcgatgaaa cgctgggctg
    12961 atgcccgggc ggatacactc gcgacgttcg acggcgtgga cttcttgtag gggtcaagac
    13021 ggacggtccc tcaactgcca cagcgggtcg tctttgaagt ggatgttgtt gatctgcagc
    13081 cagtgcctct cggcggtgtc gcccctcttc tgccagggtc gagtaagtcc aacgctcagg
    13141 atcacctcga cgcctgactt gaggcgcttt tccacgctgg caacgaggtc ccggcggggt
    13201 gttcgtccgt cccgctcgta cagccgcagg tcgttcacag ccaggtctac ggagggcatc
    13261 aggtagtcga gaaccagccg caccgtgccc cgatggtcca tgtacagcca cggttgttcc
    13321 tccggcttca ggcagcccag tgaggcgcgc cctttcccaa catccaccgt gccgctttcg
    13381 tcccacagct ccagatcggg accgaagatc ccctccaggc tctcctttgc cgacccatcg
    13441 atggcgcccc agtagtcgtc ggggtcgtcg tcgaagagcc gccgcgcgcg cgacggctcg
    13501 aagcgatggt cctccagttc gggggcgtgt ccggcgtagg tcgccggccc gaggtccact
    13561 acggacccgg cgtcgaaggg tccgccgccg gtactgagaa ggtcgatggt gagcctcctg
    13621 ccgtggccga ggaccgggcg aatgtggcgc ggagtgtcca cgtcgatacc agccacacag
    13681 aagtagcctg gctgcatccg cgtcaggtgg tttacgagta tctgcacata ccccctacag
    13741 gtgaactgcc ctgaagtcct tccatgcccg gcccaggtac tccagtacaa gacggcggtg
    13801 gcaatgctcg gcactggcct cgctgcacag cagcaccgtc ggaacttcga agaggcttct
    13861 atccagggtg tcttcgaccc ggcgctcccg cagcagatcc aggaacgccc tttcgtaatc
    13921 gggccatccg ccgcccttcc tgtaggcgtc gaggatctcg cgtgtagggg ccagtctggg
    13981 ctcataaacg tactccgcgc cgcagagcct ctgcagaaag aacgggaggt cggcgcgacg
    14041 ggcgaatccg gcgagccgcg acgcgttgtt caggcgcacg tcgaggagac gctcgatgcc
    14101 ggcgtcattc aacgctccga agaactcggg cgccgtcttc ttcgtgaaac cgatcgtata
    14161 gatttccgtg ggctcacctc cgtctccggg aacaggaccg gttgtccgcc gtccatctct
    14221 ccctctatgt gaatgctacc gtcgcccctg atgtgccgca gcacgaaccc tcgttcctcc
    14281 aacgcccgcc cgacgagtaa cctgcggtgg caacgggcgg ggtcttcctc gctgcagagc
    14341 aggacgaccg tacgggtgct gacttcctgc tccagcctac ttataccatc gaggaacggc
    14401 cgggaacacc cgacctgcgt gtagtccacc cgccccttcg cgtcgtagaa ctctatacct
    14461 gcgggccttc cgcccagctc cctgcccaag aacaggtatc cgatgccaac cttcgacaga
    14521 accgcttcga tggcgcaggc gttgaactgt ggcgcatgcc tggagtacgg gtgagaacgg
    14581 gtgtccacaa cagcttcgat cccatgcctc ctcaacagtc cggcgaactt ctccgccgaa
    14641 tggttggaat gcccgaccgt gaacaccgag ttcgcctctg tcccttggtc tccgatagcg
    14701 attcctttca gtccactgaa accctacgaa cggtatcgag aataatcccg aggccgcatt
    14761 ctccccagac ctactttcga gtatcgacac ccaagctcgc aaagagaccc atgtgttgct
    14821 cctcgaaggt ccgcacgtcg cccaggcaac atcagagccc gaagtctggc aaacgacccc
    14881 tcaccctctg tggccgccga tatctttgct cggaaggtga tcactttcac cagagcgtcg
    14941 agtctggacg ctacaaactc gggtttgagt cgggacattt gctacactca gtagcgaagg
    15001 ttttgagagt cgcatatccg gagtgagagt tttcgagcgt ggtcgggagg acgttgggct
    15061 gagaacgtcc gtagcctccc tcatttgccc gtcctgtagc gtcgcgatta tacggcggcg
    15121 ttcgtctcgg ctcacaagtc ccggattatc cgggaaggag agttacgcta catagggttg
    15181 tagggccgcc cgttatggcg gccgggcaca aaagcacatc tgcaggagaa gatttcgccg
    15241 tccctgatgt ccgctgcttt gcggtaggag gtggcgaatg aagtctttgc gggtcaacgg
    15301 aaggttgtct ttcatcgtgc tgctcatctc gcagctcgcc gcgacgatgg ggttcacgtt
    15361 cgttctgccg ttcatgccga tctacgtcca ggagctcggc gtcgaggatc ctggtagcgc
    15421 ggcggcgtgg gccggggtga tcaacagctc gaccgccgtg acgatggccc tcgccgcacc
    15481 gctgtggggc aggctcggcg accgcgtcgg gctcaagccg atgttactca gggccacgat
    15541 cgcgggatct ctcctgctcg gcctgatggg gctcgtggga agcccgtggc agctcctggc
    15601 cctcaagacg ttgcagggat gcgtgactgg tacggtcgcc gcggcgacgg tcctcgtatc
    15661 ggcgacggcc ccgccaaaca gggcggggga gaggctgggg accctgcaaa tggtcatctt
    15721 tgtcgccgcc gctgccggac cattcttcgg tggagcattc gccgacctcg tcggcatcag
    15781 ggcctctttc ggggtgaccg ccgccttgct tgcggcgtct gccgttttga tctccttctg
    15841 ggtaaaggag gagcggcccg aggaagagga ggtcgaggga gaggagtcta gcgacgccga
    15901 aggtccgctg ccgcacagac ggctcgcgcc gacacttttg gcgcttttcg tggtgcaggt
    15961 cgcgatcatg agcgcggcgc ccgcgctgcc gggtttcctc tccaccttga tggaagagcc
    16021 cgtgcgggtc gcaacccttg cgggttggat catcgcgacc ggcgccctgg ccgcatcgct
    16081 cggctccgtc atcggaggaa agctcgcggc gagattcggg gcgcggaagg tcataatctg
    16141 gaccctcttg ctggcaggca tctctgcgct gccgcaggct gaggtcgact ccgtcgcgct
    16201 gctgtgggtc ctgcgtctgg taacgggact ctttgtaggg atcgccgtcc ccgtcgccaa
    16261 cctcgcgatc cgcggcgccg tacaccccag acgccagggc gaggcgttcg gcgtcgcggc
    16321 tacggccacc tcggcgggca acgccgtcgg gccggcggcc ggtggcctgc tggcctcatc
    16381 gttcggcttc ggcgcgcctt tcctggtccc cgggctctgc ctggccgccg tctccgccat
    16441 catctggcta gcgccgctgc tcgccgtcgg ttatcggacc gcggtgcgtg atctctagcc
    16501 tcttcgagtt cgtagaagag catacgtgct ccccagcacg cgcttctggg aggaaggccc
    16561 cggctacaga aagatcggca ccggcgaacg gtagaccagg accaaaaggt cgcccaggag
    16621 ggcgccgccg tcttctaacg aaccgcctgc gccaggcccc ctaccggcgt ccctccgggc
    16681 cgcctccgaa cagcgtcttg cgggtcttgt catccatttc gtcgagcgag cggtgtagtt
    16741 ccttgcctac ctcgagggcg cggctgacgg cgggtcgcga ctccatagcc tcgtaccagc
    16801 gcctgaggtc agggtagtcc tccaggacct ggccctgctt ctcgtgtgag acgagccagg
    16861 ggtagatcgc catgtcggcg atcgtgtact cttcgccggc gaagtactct gcctccgaga
    16921 ggcgctggtc taccaccctg tatagcctgg cggcctcgtc cgtgtatcgc tctattgcat
    16981 aagggatctt ctcgggagcg tagcccctga agtggtgcgc ctggccgagc atcggcccga
    17041 tatgtcccat ctggaacatc agccactgca gcaccaggta cctgtcgcgc ggagcttcgg
    17101 gtaggaactc acccgttttg tctgcgaggt agatcaggat tgctcccgac tcggagagtg
    17161 acatgggctc tccgtcagga ccttccgggt ctacgatggc aggtatcttg ttgttcgggc
    17221 ttatcttcaa aaactccgac tcgtactggt cgcccgaagt tatgtccacg ggcaccagat
    17281 tgtaagggag tcctgcttct tcgagaaaga tcgtaatctt ccagccgttg ggagtcggcc
    17341 agtagtagag atcgatcggc tgcgccattc gcttctcctc gctctctgtt ggtaccttac
    17401 agaagttcag ccctagttcg agagcatagt ataggagaag tgggctacct tcagcccgcg
    17461 cggcagagga gcacgatcct caacatgcca tcatgtgggc ggccaaacgc ttgctgctac
    17521 catagcggtt gccagggtac gtactcgcgc accgctctcc cgctcctgga ctcccttgcc
    17581 aggtcacgaa acatcgcaag gacctcttcc ttctggtcct cctccttggc ggacgtgctc
    17641 gtgccctccc acagcgcctc catccagcgc aggttccttt ccctggctct ggccttctgt
    17701 gtgtcagtct ctctcatgcc tggactccct cctcgggtta catagacact acccgaagac
    17761 gcgtgaattt accctaccag cctagcagtt cgcacagact catacatgaa acacgttatg
    17821 tatcatacat cccggaaaca accccaagat gtgggtgatc ttacccaggc catagccttg
    17881 cgcgcggtgc tacagagtac actcggtgcc tgcaactagt ggggtagagc agcgtatcat
    17941 acagagaccg agcaacagag agaggcttac gatggatttg ctacgcatca ttatcgccat
    18001 acttctccct cctctggggg tgttcttaca ggtggggatc ggcaagcaat tctggatcaa
    18061 catcctgctg acgctcctcg gttacatccc tggcatagtc catgccgtgt gggtcatcgc
    18121 gaaatattag ctccagtggt ccttcaggcc gtgcgccgga tcaggacgat cgtcgacagg
    18181 cagaccgccg taagcgcgag gtaggagagg gcatcgcctc ggagatccgc aaggaagccc
    18241 cccaaaggag ccccgacggc ataaccggag gcccaggcga agttcgtgat cgcgaaggca
    18301 accgcccttt ccatcccggc cttctcggtg ccgcgcgaga agagcgccgt acctgggacg
    18361 agcgtcgcgt tgaacgcccc tgccgcgagc acgactagaa gcccgatcat ccacgggttc
    18421 ccggcccacg tcaggaggag caggatgccg atggaggcga gcaaaccggc gaagacgggc
    18481 gggcgatagc cagagcggtc ggaccagcgg cccagcagcg gatggacgaa agcctcgaag
    18541 atggcgctta cgaaaaagac cgtccccacc gccgcagcgc cccatccgag ccggttcaga
    18601 tccaacggca cgaggacggc cagcgcgctg aaaagaaggg gggagagcac gataaaccag
    18661 aggctggcga gcagcagggg ctcccttacc gtcctgagag ccggtagcaa ggacacgtct
    18721 cccgaggagg ccggcgctcg ctctagggcg gcccagacgg cgatgacccc gccggcgatg
    18781 gcgacggatg cgaaggcagg caagaggccc acggtcgctg cgacgctccc gagtaccggg
    18841 cccaacagcg cccctaccac ggcagcgctt accatcaccc cgatcatctc ggcccgccg
//
LOCUS       DATMVF010000034         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_600106, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000034 DATMVF010000000
VERSION     DATMVF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600106"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VN178_01410"
     CDS             complement(<1..208)
                     /locus_tag="VN178_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HWS79650.1"
                     /translation="MYGYRPTTRLLSMLELLQARGRVGGPELARRLEVGERTVRRYVA
                     MLQEMGVPVEAERGRYGAYTLRPGF"
     gene            464..685
                     /locus_tag="VN178_01415"
     CDS             464..685
                     /locus_tag="VN178_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79651.1"
                     /translation="MNDWLSVELARERNARILAEAEERRLARLVPRKSVRSALRARVA
                     RKMFQLAVALEKEETWRAVWERLEAPRHP"
     gene            complement(731..1942)
                     /locus_tag="VN178_01420"
     CDS             complement(731..1942)
                     /locus_tag="VN178_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWS79652.1"
                     /translation="MKISDLRAICTAPEGIRLVVVKVETDEPDLYGLGCATFTQRPLA
                     VVTAVEEYLKPFLVGRDPDDIEDIYQSAYVSSYWRGGPVLNNALAGVDMALWDIKGKR
                     AGMPVHQLFGGKCRNAVALYAHATGNDFSEVEDEVRKYMEDGYRYVRCQISVPGYSTY
                     GSTISAGPQTLGLGDDPWEPTPYCRLIPRLFEHLRSSLGDEVELLHDVHERVPPIQAI
                     GLARELEPYNLFFLEDPLAPEDAEYFRLMRQRTATPIAMGELFVNQAEYVPLIRDRLI
                     DFIRVHISDIGGLSMARKLAAYGEFFGVRTAWHGPGDVSPVGHAANLHLDLACHNFGV
                     QEQHVFDERTREVFPGCPEIRDGYMWPSDSPGLGVDIDERLAAKYPFPEDPLNGGWPP
                     VRRLDGTVIRP"
     gene            complement(1939..>2809)
                     /locus_tag="VN178_01425"
     CDS             complement(1939..>2809)
                     /locus_tag="VN178_01425"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWS79653.1"
                     /translation="MQEIEGVYVANVTPFRDDGTLAVDEEAYLEHVAWLAEMGVRGVV
                     PFGTNGEGPSVTLGEKLRVLEALFGRGFPLQIIPAVMQSNLPESLEMLRALEDYPATA
                     VLVLPPYYFKPVEPEGMVRFYEPVLGATGHTTIIYHIPKYAVPVPDRVVIELPVWGVK
                     DSGGDEAYAESVLQGGKGVLLGTEDGLWRKLDTGAQGVISALANFIPEEVLELYEKHG
                     EGDEVEGTALSEKLQRARAMTKEYASPAVLKRLAEARHGARMGTVRPPFVPVPADYDP
                     NPALLAAGHGAGR"
BASE COUNT          481 a          894 c          916 g          518 t
ORIGIN      
        1 tgaaaccagg cctcagggta taggcgccgt acctgcccct ctctgcctct acgggcacgc
       61 ccatctcctg gagcatcgcc acgtagcggc gcacggtcct ctcgccaacc tccagcctgc
      121 gggccagctc cggaccaccc actctccccc gcgcctggag caactccagc atagagagca
      181 acctcgtcgt cggacggtag ccgtacatgg tcgctccctt cggtcgctca gcatttcagc
      241 ttgtcagcac gctcaggctt ttagccttcg cttgtcagca tttcagcttt cgcatgggag
      301 atcctcgctg acctgctgac aagccgcccg cagggcggcg tgctgacaag cggagcccga
      361 agggcggagc gtgctgaatt gctgaagtgc tttcatatca ggaaggatta tgacctcttt
      421 cgccagtatt ctcatccggc aagcgagaga ggagataggc gccgtgaacg attggttatc
      481 tgtggagctc gcgagggaga ggaacgcaag gatcctggcc gaggcagagg agcggcggct
      541 tgctcggctg gtgcccagga agtcggtccg gagcgcgctg cgggcacgcg tggcgaggaa
      601 gatgttccag ctcgcggtgg cgctggagaa agaagagacc tggcgcgccg tatgggagag
      661 gctggaggcg ccgcgtcacc cgtgagacgg aacccggttg gagggttccg cgtccggtca
      721 tcccgctaac tcacggtcgg atcaccgtcc cgtcaaggcg cctgacggga ggccagcctc
      781 cgttcaacgg gtcctcgggg aacggatatt tggccgcgag acgttcgtct atatccacgc
      841 cgagcccggg cgaatcggag ggccacatgt aaccgtcccg gatctccggg catcctggga
      901 agacctcgcg ggtgcgctcg tcgaagacgt gctgttcctg gaccccgaag ttgtggcatg
      961 ccaggtcgag gtgcaaattg gcggcgtggc ctaccgggga gacgtcgcct ggcccgtgcc
     1021 acgccgtcct cacgccgaag aactcgccgt acgccgcgag cttacgggcc atgctcaggc
     1081 cgccgatgtc cgagatgtgt acgcggataa agtcgatcag ccgatccctg atcagcggca
     1141 cgtactcggc ctggttgacg aaaagctccc ccatcgcgat cggcgtggcg gtccgctggc
     1201 gcatgagacg gaagtactcc gcatcctcgg gggctagcgg gtcctcgagg aagaaaaggt
     1261 tgtagggttc gagttctctg gcgaggccta tggcctggat cggcgggaca cgctcgtgga
     1321 cgtcgtgtaa cagctcgacc tcgtctccca gggagctgcg caggtgttcg aagagacgag
     1381 ggatcagacg gcagtaaggt gtcggctccc aggggtcgtc tcccagtccc agcgtctgcg
     1441 gtcctgctga gatcgtggac ccgtacgtcg agtagcccgg cacggagatc tggcaccgca
     1501 cgtagcggta accgtcttcc atatacttcc tgacctcgtc ctcgacctcg ctgaagtcat
     1561 tacccgtggc gtgggcgtag agggccaccg cgttgcggca cttgcccccg aagagctgat
     1621 gtaccggcat cccggccctc ttgcccttta tatcccacag cgccatgtcc acgccggcca
     1681 gggcgttgtt caggactggg ccaccgcgcc agtatgagct gacgtaggcc gactggtaga
     1741 tgtcttcgat gtcgtccggg tcgcggccga cgaggaaagg cttcagatac tcctcgacgg
     1801 ccgtgacgac ggcgagcggg cgctgggtga aagtggcgca cccgagaccg tagaggtccg
     1861 gctcgtcggt ctcgaccttc accaccacga gccttatgcc ctcaggcgcc gtacagatag
     1921 ctctcaggtc ggagatcttc atctgcctgc tccgtggccg gcggccagca gtgcggggtt
     1981 gggatcgtag tccgcaggga caggcacgaa ggggggcctg acggtcccca tccgcgcgcc
     2041 gtgcctggcc tccgcgagcc tcttgaggac cgcgggggag gcatactcct tggtcatcgc
     2101 gcgcgctctc tgcagctttt cggagagggc cgtaccttcg acctcgtcgc cttctccatg
     2161 cttctcgtag agttcgagca cctcttccgg gataaagttg gccagggcgg agataacacc
     2221 ctgggcccct gtatctagct ttcgccacag gccgtcctcc gtgccgagga gcacgccttt
     2281 gccgccctgg agaacggact cggcgtaggc ttcgtctccc ccagagtcct tgacgcccca
     2341 gacggggagt tctatcacga cccggtcagg aacggggacg gcgtacttcg ggatgtggta
     2401 gatgatggtg gtgtggcccg tcgccccgag cacgggctcg tagaagcgca ccatcccttc
     2461 cggttccacg ggcttgaagt agtagggcgg caggacgagc acggcggtag caggatagtc
     2521 ctccagggcg cgcagcatct ccaggctctc gggcaggttg ctctgcatga cggcggggat
     2581 gatctggagt gggaacccgc gtccgaacag cgcctcgaga acccgcagct tctcaccgag
     2641 cgtcaccgaa ggtccctcgc cgttggtgcc gaaaggcacc acgccccgca cgcccatctc
     2701 cgcgagccag gcgacgtgtt cgaggtatgc ttcctcgtcg accgcgaggg taccgtcatc
     2761 cctgaacggc gtcacgttgg cgacgtagac gccttctatc tcttgcatc
//
LOCUS       DATMVF010000035        10051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_280036, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000035 DATMVF010000000
VERSION     DATMVF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280036"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1537
                     /locus_tag="VN178_01430"
     CDS             215..1537
                     /locus_tag="VN178_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS79654.1"
                     /translation="MKRMLTRRGFLKAGGGALAGAYILGLAGCGGGDEGGSGDNTLEF
                     WAFDEGRADLARAAIKTQEWQDTHGNVEVNFRIFPYEQMHDKLLTALVSGKGAPDVAD
                     VEISRFSNFIKGDELPFVDLTDRIGDDINDVYKPAATDPWTWQNKIYGVGNELNTCVL
                     AYRTDVMEEAGVQTPFESWDEVIEAGRVVSNDERKMFALHDIAFGDHYMLSQSSGTTY
                     FDENGEYIGDNEKSVGALQFLHDMVYDSGIAGIAPVVANDDWYPPQYRAAFRAEKFIA
                     LFGPPWHVSFLFTDVPEQSGKWGVQKLPSGLGEGLPTANFGGTGQCVTTQSQNVDAAY
                     DLVRISNLTTEGVMADFKARTAYPAYKPAYDEPALQEPNEYFGGERIGQLYAELAPEL
                     PPFNQSTVWGQATEALVREAITPVMQDQMDAKAALTELRDTIESMKQG"
     gene            1578..2534
                     /locus_tag="VN178_01435"
     CDS             1578..2534
                     /locus_tag="VN178_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS79655.1"
                     /translation="MERQSDQESIAVRQGEGLAPRKRGRGGARGTGSFTPYLFLLPFF
                     LIYLVFLVYPVLAAFRLSFYESSGFGGDTFTGLDNYARLVQDSRYLEALKNTTLYALA
                     SVFILSPLALLVAVAIRSFVVPIATFKSFYRIIFFLPFITSFVVIALMFNLVFNNEFG
                     LLNNGLSALGLPTLDWLRDERVALLAIILVGIWTYLGINALYFLAGLQNIPEEVIEAA
                     RVDGANRFQVFWRVTLPLLRPVILFVVVQATIFSYQIFEIPFLLTNGGPSDATLTLAI
                     YLYEVGFREFDRGYAAAIGYSMAIIAIVLAAIQLLAARRFDR"
     gene            2547..3383
                     /locus_tag="VN178_01440"
     CDS             2547..3383
                     /locus_tag="VN178_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS79656.1"
                     /translation="MVGNKLQMRLWQVLGNVILLFAVFLLVMPYLYMISASFKEGNEI
                     FSIPIKVLPQGLYLGNYDLLFTETNFVRWFFNSVFIGLSRAALAIVVSIMAGYAFAKF
                     DFRFKNALFLFVIATLTLPIYVLIVPLYDMMVTLGWTDRYVALILPFAAQAIGVFLAR
                     QYLLSVPDEILDAARVDGASEWGVFWRVVLPLTTPVMAVLGILFFTTAWNDFIWPLVV
                     MTEDNKFPVALGLPTLLGPYSQEYGAVMAGSFLSTLPIIIVFLIAQRRFIEALTVGAV
                     KG"
     gene            3419..4825
                     /locus_tag="VN178_01445"
     CDS             3419..4825
                     /locus_tag="VN178_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWS79657.1"
                     /translation="MANGTDMLAVRGNRIVDGEGNPIVLRGFGLGGWMNMENFITGYP
                     ANEEGMREAVRAVLWEEKYEIFFDRFLEHFFTEGDARFISSLGLNLLRLPVNYRHFED
                     DMRPFEIKEEGFRHLDRVVELCAAHGIYTIIDLHALPGYQNQHWHSDNPTHKALLWRH
                     KHFQDRTVNIWEHIAERYRGNPWVAGYNPINEPADPSERLIMPFYRRLYEAIWAVDSD
                     HLIFLEGNRYSVDFHMFGEPWPGVVYTNHDYALPGFIDGGPYPGVSRGEYVDRDVLER
                     TFLQRSSYMLEHDVPLWVGEFGPVYLGEEEADRMRYQLLRDQLEIYDRHGVSWAIWTY
                     KDIGLQGVQYAAPDSPWVECIRPILEKKTRLGVDAWGGTDAGVRHVMAPLEELFANEY
                     PNYKPFPFGAEREIELLVRNILLAEPMLPEYGELFMGMDEGEIDAMMRSFSFENCVRR
                     EALASILSEHAVGSGARG"
     gene            4858..6219
                     /locus_tag="VN178_01450"
     CDS             4858..6219
                     /locus_tag="VN178_01450"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HWS79658.1"
                     /translation="MNFPVNFLWGTATASYQVEGAVNEGGRGASIWDTFSHTPGKVVH
                     GDTGDIACDQYHRLEEDLDLMSELGMQAYRFSVAWPRIQPEGSGPANEEGLDYYRRLV
                     DGLRQRSIEPMLTLYHWDLPQALEDLGGWANKDTSERFAEYAGIVYEALSDSVGFWIT
                     LNEPWVLAWMGYGLGVHAPGISDASKALAATHHLLLGHGLAMEKMRSAGDDNQLGVTL
                     NLHPAHPSRDRDADREAAHRVDGQANRLYLDPLFRGDYPEDVFSHYRERGADLSFVHE
                     GDLSKISAPIDFLGVNYYFRNTVRDAPGEDAPGTPFADLDARSVIPHEAEKTAMGWPV
                     EPDGLTEILVRIKEEYADLPIYVTENGRAVHDYIDPEGDVDDEERVSYLDAHFRAAHE
                     AMEQGVDLRGYMVWSFLDNFEWAEGYSKRFGIVFVEYGTQRRVPKSSARWYSDVVRRN
                     GLE"
     gene            6244..7230
                     /locus_tag="VN178_01455"
     CDS             6244..7230
                     /locus_tag="VN178_01455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS79659.1"
                     /translation="MEYRDLGRTGMRVSEISLGTWAFGGDWGTVGEDDAYAALNRAVD
                     LGVNFLDTADVYGDGRSEKLIGRLLKDHPNDEILVATKAGRRLDPHTPEGYDYDNLSA
                     FVERSLNNLGVEALDLLQLHCPPTEVYRQDSTFEALDQLREAGKLLNYGVSVEKVEEA
                     RMALDYPGVATVQIIFNIFRQKPAEEFFPLAEERNVGILARVPLASGLLSGKMRSDRV
                     FDEDDHRKFNREGQAFDKGETFSGVDFETGLLAAEELKELVPEGNTLAQFALRWILMH
                     PAVSCAIPGGKNASQVEDNVTAAEMPLLSDDVMRRAREIYDHRVSEEVHHKW"
     gene            7278..8879
                     /locus_tag="VN178_01460"
     CDS             7278..8879
                     /locus_tag="VN178_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS79660.1"
                     /translation="MEAETDILVVGGGLGGVAGALAALRLGRRVVLTEETDWIGGQLT
                     AQAVPPDEHPWIEDMGCTASYRRLRNGIRYFYRRNYPLTAGAHAREHLNPGDGLVSRL
                     CHEPRVSLAVLEGMLAPYRSGQRLRVLLRHRPVAAEVEGDLITAVTLLDGETGEQLTV
                     HASYVLDATELGDLLELAGVEHAVGAESRDETGEPHALPKDPNPLDQQAVSWCFPLDY
                     MPGEDHTIDRPEDYDYWRSYRPNFWPGPLLSWTDVYPDTLQTRQRAIFHEEPFFAGRD
                     GSDLWHYRRILARENFSEGLLDSDITLVNWPQIDYWLDPLVGVGEAERREHMQSARQL
                     SLSMLYWMQTEAPKLDGGVGYRGLRLRPDVVGTSDGLAKHIYVRESRRIRAEFTVLEQ
                     HVGVEARGGSRGAQSFHDSVGIGAYRIDLHPSAAGRSYVDVSSWPFQIPLGALIPVRV
                     KNLLPACKNPGTTHITNGCYRLHPVEWNIGEASGALAAYCLDKDLVPRQVREDVENLE
                     DFQRLLTDVLGFELAWPEPQRYESL"
     gene            8963..>10051
                     /locus_tag="VN178_01465"
     CDS             8963..>10051
                     /locus_tag="VN178_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79661.1"
                     /translation="MIPIPRPEYPRPQFRRKDWTNLNGEWSFAFDDSETGLTNGWQSA
                     TPGDLRSGNSPFDRKITVPYCFQSKLSGIGETDFHDIVWYARAFEYAPAADERLLLHF
                     GAVDYRATVWVNGTQVASHEGGHTPFSADVTYALAGEAADNIVVVRAEDPSQDVTIPR
                     GKQYWKEKSEGIFYTRTTGIWQTVWLEPVNRRRIATLRMTPDVDSASVELEVSLRGIE
                     PGMSLRVTVELDGERVLEDTLSVPSSLVERSLPLLRRGEAPDTPHVADWPGPALWSPE
                     HPNLYDLRLELLDPDGGVLDRVESYFGMRKLEVKDGKVFLNDRPLYQRLVLDQGYFPE
                     GVLTAPTDEDLRKDIELAREMGFNGARKH"
BASE COUNT         1946 a         3130 c         3115 g         1860 t
ORIGIN      
        1 acgcgttgtc tatattgccc agcgtaagct ttcggggtcg aaagacttga tctggggaaa
       61 gacaggtacg aggaggccag agacgagcgc gcttctgctg cgccctttgg tgttcgttac
      121 ggtgcgctca cgtgtgccgc gacctttcgc gaccggttcc cgagggcatg ggtagggacc
      181 tggtaaaagg gttcgttcgg gtcaggagga cgacatgaag cggatgttga cgaggcgtgg
      241 ctttttgaag gcgggtggag gagcgctggc cggcgcctac atcctcgggc tagccggctg
      301 tggtggcggt gatgagggtg gctcgggaga caacactcta gaattctggg ccttcgacga
      361 gggacgtgcc gacctcgcgc gggcagcgat caagactcag gagtggcaag acacgcacgg
      421 aaatgttgag gtgaacttca ggatcttccc gtacgagcag atgcacgaca agcttctgac
      481 ggcgttagta tccgggaaag gagccccgga cgtcgctgat gtcgagatct cgcgtttctc
      541 caactttatc aagggcgacg aactgccgtt cgtggaccta accgatcgca tcggcgacga
      601 tataaacgac gtctacaagc cggcggcgac cgatccctgg acctggcaga acaagatcta
      661 cggggtcggc aacgagttga atacctgcgt ccttgcctat cgcacggatg tcatggaaga
      721 ggctggcgtg cagaccccgt tcgagtcctg ggacgaggtc atcgaagctg ggcgggtggt
      781 ctcgaatgac gagcgcaaga tgttcgccct gcacgatatc gcctttggcg accactacat
      841 gctttcgcag agctccggga ccacgtactt cgacgagaac ggtgagtaca tcggggacaa
      901 cgagaagagc gtcggagcgt tgcagttcct ccacgatatg gtctatgact ctggtatagc
      961 cggtatcgcc cccgtcgttg cgaacgatga ttggtaccca ccgcagtaca gggcggcgtt
     1021 cagagcggag aaattcatcg cgcttttcgg acccccgtgg cacgtgagct tcctctttac
     1081 ggatgtgccc gagcagtcag ggaagtgggg tgtgcagaag ctcccctccg gtctcggcga
     1141 gggactgccg actgcgaact tcggcggcac cgggcagtgc gtgaccacgc agagccagaa
     1201 cgtcgacgcg gcctacgatc tggtgcgtat ctccaacctc actaccgaag gcgtcatggc
     1261 ggactttaag gcccggacgg cgtaccccgc gtacaagccg gcctacgatg agcccgccct
     1321 gcaggagccg aacgagtact tcgggggcga gagaataggc cagctctacg ccgaacttgc
     1381 ccccgaactt ccaccattca accagtcgac tgtgtggggc caggccacag aagcgctcgt
     1441 ccgtgaggca ataacccccg tcatgcagga ccaaatggac gccaaggccg ccctcacgga
     1501 actgcgcgac accatcgaga gcatgaagca aggttagatc catggggccc gtggtctgag
     1561 tctcttagga ggcagagttg gagcgccaat ccgaccaaga gagtattgcg gtacgtcagg
     1621 gggagggatt ggcgcctcgc aagcgcggac ggggaggtgc ccgcggcaca gggagcttca
     1681 caccttacct gttcttgttg ccgttcttcc tgatctactt ggtattcttg gtctacccgg
     1741 tgctcgccgc gttccgtttg agcttctacg agtcctcggg ttttgggggt gacacgttca
     1801 ccgggctaga caactacgcc aggctggtgc aggattcgcg ctacctggag gcactcaaga
     1861 acaccaccct ttacgccctg gcgagtgtct tcatcctgag cccgctggcc ctgctcgttg
     1921 ccgtagcgat ccgctcgttc gtcgtgccga tagcgacctt caaaagcttc taccgtatca
     1981 tcttcttcct acctttcatc acgtcgttcg tcgtcatcgc cctgatgttc aacctcgtct
     2041 tcaacaacga gttcggcctc ctcaacaatg gcctctcggc tttggggttg ccgacgctcg
     2101 actggctgcg tgacgagaga gtggcgctgc tggcgatcat cctggtaggc atctggacgt
     2161 atcttgggat caacgcgttg tactttctgg cggggttgca gaacataccc gaggaggtca
     2221 tcgaggccgc gagagtggac ggggcgaaca ggttccaggt cttctggcgg gtgacgcttc
     2281 cgcttctgcg acccgtaatc ctcttcgtcg tcgttcaggc gacgatcttc tcctatcaga
     2341 tcttcgagat accgttcctg ctcaccaacg gcgggccctc agatgcgacg ctgacgctcg
     2401 ccatctacct ctacgaggtc ggcttccggg agttcgaccg gggctacgcc gccgcaatcg
     2461 ggtactcgat ggccatcata gctatagtcc ttgccgcaat acagctcctc gcggcccgca
     2521 ggttcgacag gtagggagtg ggaagaatgg tcggcaacaa gctgcagatg cgcctttggc
     2581 aggtgctcgg caacgtcatc ttgctgtttg cggttttcct actggtgatg ccctacctgt
     2641 acatgatcag cgcctccttc aaggagggca acgagatctt ctcgatcccg ataaaggtcc
     2701 tgcctcaggg cctctacctg ggtaactacg atctcctctt cacggagacc aacttcgtgc
     2761 gctggttctt caacagtgtc tttatcggcc tttcgcgggc cgctttggcc atcgtggtct
     2821 caatcatggc cggttacgcc ttcgccaagt tcgacttccg cttcaaaaat gcgcttttcc
     2881 tcttcgtcat cgcgacgctg acgttgccca tctacgtcct tatagtcccg ctctacgaca
     2941 tgatggtcac tctgggctgg acggaccgct acgtcgccct gatcctaccg ttcgcggcgc
     3001 aggccatcgg cgtcttcctg gcgcggcagt acctcctctc cgtcccggac gagatcctgg
     3061 acgcggccag ggtggacggg gcgtcggagt ggggggtctt ctggcgggtg gtcttgcccc
     3121 tcaccacgcc tgtgatggct gtccttggga tcctcttctt cacgaccgcc tggaacgact
     3181 ttatttggcc actggttgtg atgacggagg acaacaagtt cccggtcgcc ctgggactgc
     3241 cgacgctgct cggaccgtac agccaggagt acggggcggt catggccggg tccttcctga
     3301 gcacgctccc gatcatcatc gtcttcctga tcgcgcagcg ccgctttatc gaggcgctga
     3361 cggtcggggc agtcaagggc tagagatccc gcaaccactt tagatagaag gagagaccat
     3421 ggcgaacggc acagatatgt tggcggtacg aggcaaccgc atcgtggacg gcgaggggaa
     3481 ccccatcgta ctgcgtggct tcggcctcgg cggctggatg aatatggaga actttataac
     3541 aggctaccct gcgaatgagg aggggatgcg cgaggccgtc cgcgcggtgc tttgggagga
     3601 gaagtacgaa atcttcttcg accgtttcct cgagcacttc ttcacagaag gtgatgcgcg
     3661 attcatctct tccctcgggc tgaatctgct cagacttccc gtgaactaca ggcacttcga
     3721 ggacgacatg cgccccttcg agatcaaaga ggaaggcttc aggcacctgg acagggtcgt
     3781 agaactctgc gcggcgcatg gtatctacac gatcatcgac ctgcacgccc taccaggcta
     3841 ccagaaccag cactggcact ctgacaaccc tacccacaag gcgctcctct ggaggcacaa
     3901 acacttccag gacaggaccg tgaacatctg ggagcacata gccgaacgct acagaggcaa
     3961 cccgtgggtg gctggctaca accccataaa cgagccagcc gacccgagcg agcgactcat
     4021 aatgcccttc tacaggcgcc tctacgaggc gatctgggcc gtcgactctg accacctgat
     4081 cttcctcgag ggcaaccgtt actcggtgga cttccacatg ttcggtgagc cgtggcccgg
     4141 cgtcgtgtat accaaccacg actacgcgct gccaggcttt atagacggtg gcccctaccc
     4201 cggtgtctcg cgcggcgaat acgttgacag ggatgttctg gaaaggactt tcctacagcg
     4261 cagctcttac atgcttgagc acgacgtacc cttatgggtg ggcgagttcg ggcccgtcta
     4321 cctgggcgag gaggaagcgg accggatgcg ctaccagctc ctccgcgacc agctggagat
     4381 ctacgaccgc cacggcgtga gctgggccat ctggacgtac aaggatatcg gcctgcaagg
     4441 agtccagtac gccgccccgg actctccgtg ggtcgagtgc atacgaccca tactggagaa
     4501 gaagacccgc ctcggcgtgg acgcctgggg cggtactgac gcaggcgtca ggcacgtcat
     4561 ggcaccgctc gaggagctgt ttgccaacga gtacccgaac tacaaaccat tcccgttcgg
     4621 ggccgagcgt gagatcgaac tgctcgtacg caacatcctg ctcgccgagc cgatgctccc
     4681 cgagtacgga gagcttttca tgggtatgga cgagggcgag atagacgcga tgatgcgctc
     4741 gttctccttc gagaactgcg tgaggcggga ggcgctggcc tctatactct ccgagcacgc
     4801 cgtaggtagc ggcgcgcgcg gttgagcacc gccggcgcag tagacggagg tacaggcttg
     4861 aacttcccag tgaacttctt gtggggtacg gcgacggcct cctaccaggt cgagggcgcc
     4921 gtgaacgaag gcggccgcgg cgcgtcgatt tgggatacat tcagccatac tcctggcaag
     4981 gtcgtccacg gcgatacggg cgacatagcc tgcgaccagt accaccgtct ggaagaagac
     5041 ctcgatctga tgtccgagct cgggatgcag gcctaccgct tctccgtggc ctggccgcgg
     5101 atccagccag aggggagtgg accagccaac gaggagggcc tcgactacta caggcgcctc
     5161 gtcgacggct tgaggcaacg ctccatcgag ccgatgctca cgctctacca ctgggacctg
     5221 ccgcaggcac tggaggatct gggcggctgg gccaacaagg atacgagcga gcgctttgcg
     5281 gagtatgctg gcatcgtcta cgaggccctc tcggacagcg tgggattctg gatcaccttg
     5341 aacgagccct gggtcttggc ctggatgggt tacggcctcg gtgtccacgc ccctggcatc
     5401 agcgacgcct cgaaggcgct cgccgcgacg caccacctcc tgctcggcca cggcctcgcg
     5461 atggaaaaga tgcgctccgc cggggatgac aaccagctcg gcgttacgtt gaacctgcat
     5521 cctgcccacc cctcgcgaga ccgcgacgcc gatagggaag ccgcgcacag ggtggacgga
     5581 caggcaaacc ggctctacct ggatcccctg ttccgcggag attatccgga ggatgtgttc
     5641 tcacactaca gagagcgggg tgcggatctc tcgttcgtcc acgaggggga cctcagcaag
     5701 atctccgctc ccatcgactt ccttggcgtc aactactact tccgcaacac cgtccgcgat
     5761 gcgcctgggg aagacgcgcc tggcacgccc tttgccgacc tcgacgcccg tagcgttatc
     5821 ccgcacgagg cggagaagac tgctatgggc tggcccgtgg agccggatgg cctgacggag
     5881 attctggtgc ggatcaagga agaatacgct gacctgccaa tctacgtgac cgagaacggg
     5941 cgagccgtcc acgattacat agaccccgag ggagacgtgg acgacgagga gcgcgtctcg
     6001 tacctggatg cccacttcag ggcagcccac gaggcgatgg agcagggcgt cgacctgcgc
     6061 ggttacatgg tttggtcttt cctggataat ttcgagtggg ccgaagggta ctccaagcgg
     6121 ttcgggatcg tgttcgttga gtacggaacg cagcggcgcg tccccaagtc gagcgcccgc
     6181 tggtactctg acgtcgtccg gcgcaacggg ttggaataga tcatacctgg aaaggacagg
     6241 actttggaat atagagacct cggacggacg gggatgcgcg tctcggagat cagcctcggc
     6301 acctgggcct tcgggggcga ctggggaaca gtcggggagg acgacgctta cgccgcgctg
     6361 aaccgagcgg tcgacctcgg cgtgaacttc ctcgacacgg cagacgtcta cggagacgga
     6421 cgcagcgaaa agctcatcgg taggctcctg aaggaccacc caaacgatga gatcctggtc
     6481 gcgaccaagg caggacgacg cttggatccc cacactccgg aaggatacga ttatgacaac
     6541 ctgtcggcct tcgtcgagcg tagcctgaac aacctcgggg tcgaagccct ggacctgctg
     6601 cagctccact gcccgccgac ggaagtctac cgtcaggact cgaccttcga ggcactggac
     6661 cagctgcggg aggcaggaaa actcctcaac tacggcgtga gcgtggagaa ggtcgaggag
     6721 gcgcggatgg ccctcgatta tcccggcgta gcgaccgtcc agatcatctt caacatcttt
     6781 cgccagaagc cggccgaaga gttcttcccg ctcgccgagg aacgtaacgt cggcatcctg
     6841 gcccgcgtac ccctcgccag cggtctcctc tcgggcaaga tgcgatcgga ccgtgtgttt
     6901 gacgaagacg accaccgcaa gttcaatcgt gaagggcagg ccttcgataa gggcgaaacg
     6961 ttctccggcg tggacttcga gaccggcctt ctggccgcgg aggaactcaa agagctcgta
     7021 cccgaaggaa acaccctcgc gcaattcgcg ctgcgttgga tcctcatgca ccctgccgtc
     7081 tcctgcgcca tccccggcgg caagaacgcc tcccaggtcg aggacaacgt caccgcggct
     7141 gagatgcctc tgctctccga cgacgtcatg cgacgagcgc gggagatcta cgaccaccgc
     7201 gtcagcgagg aggtccacca caaatggtag cctcgctggc gtcccaacgg gatgctcgcg
     7261 gagaggtgta ggcgcctttg gaggccgaga cagatattct ggtcgtcggt ggggggctgg
     7321 ggggtgtggc aggcgccctg gcggcgttgc gtctcggccg gagggtcgtg ctgaccgagg
     7381 agacggattg gatcggcggc cagctcaccg cccaggcggt cccgccggac gagcatccct
     7441 ggatcgagga tatgggctgc acggcgagct accgccgcct gaggaatggg atcaggtact
     7501 tctacaggcg caattatccc ctcactgcag gagcccatgc ccgggaacac ctcaatcccg
     7561 gagacgggct ggtgagtcgc ctctgccacg agccgcgcgt gtccctcgcc gtcctggagg
     7621 ggatgctcgc cccctaccgc tccggacaga ggctgcgggt gctgctccgc catcggcccg
     7681 tcgcagccga ggtcgaaggg gatctcataa cggcggttac cctcctcgac ggggagaccg
     7741 gcgagcagct gaccgtacac gccagttacg tgctcgacgc gacggagctc ggagatctgc
     7801 tagagctggc gggagtagag cacgcggtcg gtgccgagag ccgggacgag accggagagc
     7861 cccacgcgct gccgaaagac ccgaaccctt tggaccagca ggctgtgagc tggtgctttc
     7921 ctctcgacta catgcccggc gaggaccaca cgatagacag gcccgaagat tacgactact
     7981 ggcgcagcta ccggccgaac ttctggccag gcccgctcct ctcctggacg gacgtctacc
     8041 ccgatacact acagacccgc caacgtgcga tcttccacga agagcccttc ttcgccggac
     8101 gggacggctc cgacctctgg cactaccggc gcatactcgc ccgcgagaac ttctccgagg
     8161 gcctgctcga tagcgacatc accctggtca actggccgca gatagactac tggctagacc
     8221 ctctcgtagg cgtcggagaa gcggagcgac gcgaacacat gcaaagcgcg cggcaactca
     8281 gcctctcgat gctttactgg atgcagaccg aggcgccaaa gctcgacggc ggcgtagggt
     8341 acaggggcct gcgtcttcgt cccgacgtcg ttggtaccag cgacggcctc gccaagcata
     8401 tttacgttcg cgagagccgt cgcatcaggg ccgagttcac ggttctggag cagcacgttg
     8461 gcgtcgaagc gcgaggcgga tctaggggag cacagtcttt ccacgacagc gtgggtatcg
     8521 gcgcctaccg catcgacctg catcccagcg ccgcgggacg cagctacgtg gacgtgagca
     8581 gctggccgtt ccagatcccg ctcggagccc tgatcccggt tcgcgtcaag aacctgcttc
     8641 cggcctgcaa gaacccggga accacccaca tcaccaacgg ttgctacagg ctgcatcccg
     8701 tcgagtggaa catcggtgag gcatcaggag cgctcgccgc ctactgcctg gataaagatc
     8761 tcgtcccgag gcaggtgcgc gaagatgtgg aaaacctgga ggacttccag cgcttgctta
     8821 ccgacgtcct ggggttcgag ttggcctggc cagaaccgca acgttacgag tccctttgaa
     8881 gaagacgttg cttcctcgcg gatcaccgcc cgcgatcccc cgaatgaatt gcccgtgcct
     8941 ctagcagaaa ggtaacagta ccttgatccc gatcccacga cccgagtacc cgcgtccgca
     9001 gttccgccgc aaggactgga cgaatctgaa cggcgagtgg agcttcgcct tcgacgactc
     9061 tgaaaccggg ctcaccaacg gctggcaaag tgccaccccc ggggacttgc gctccggtaa
     9121 ctcgccgttc gaccgcaaga tcactgtccc ctactgcttc caatcgaaac tctccggcat
     9181 cggcgaaacg gacttccacg acatcgtctg gtacgcccgc gccttcgagt acgcgccggc
     9241 ggccgacgag cggctgctgc tccatttcgg tgccgtagac taccgcgcga cagtctgggt
     9301 gaacggcacg caggtagcct cccacgaggg agggcacacg ccgttctccg ccgacgtgac
     9361 gtacgctctc gcgggcgaag ccgcagacaa catcgtcgtg gtgcgggccg aggatcccag
     9421 tcaggacgtg accatcccgc ggggcaagca gtactggaaa gagaagtccg aggggatctt
     9481 ctacacccgt acgaccggga tctggcagac ggtctggctc gagcctgtga accgccgccg
     9541 catcgctacc ctccgaatga ctcctgacgt ggactcagcc agcgtcgagt tggaggtctc
     9601 tctcagaggc atcgaaccgg gtatgagtct ccgcgtgacg gtggaactcg acggagagcg
     9661 ggtgctcgaa gacaccctca gcgtgccttc ctccctcgtc gaacgcagcc tgcccctcct
     9721 ccgtcgcggc gaagcaccgg acacgccaca cgtggccgac tggcccggtc ctgccctctg
     9781 gagtcccgag catcccaacc tctacgacct gcgtctcgag ctgctcgatc ctgacggcgg
     9841 ggtactggac cgcgtggaga gctacttcgg gatgcgcaag ctcgaggtca aagatggcaa
     9901 ggtcttcctc aacgaccgtc ccctctacca gcggctcgtc ctcgaccagg gctacttccc
     9961 cgaaggcgtc ctgaccgccc cgacggatga agatctgcgc aaagacatag agctggccag
    10021 agagatgggc ttcaacggcg cccgcaagca c
//
LOCUS       DATMVF010000036        12404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_646031, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000036 DATMVF010000000
VERSION     DATMVF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646031"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(521..1462)
                     /gene="coaA"
                     /locus_tag="VN178_01470"
     CDS             complement(521..1462)
                     /gene="coaA"
                     /locus_tag="VN178_01470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HWS79662.1"
                     /translation="MDAGQNSLHPFVRLSREDWAGLPADASFALSEDDAREIGAYLSL
                     EEAREVYLPLSRLLYLHVSATQSLYRAARAFLAEEEKEVPFVIGIAGSVAAGKSTVAR
                     VLRALISRWPGRPEVGLVSTDGFLYPNSVLRSRGLMERKGFPESYDLPLLLRFLADIK
                     SGRDGVSAPIHSHLTYDILPDRTQILGRPDVLIVEGLNMLEAGLPEPSEGHRVFVSDY
                     CDFTIYVDAQERHIKEWYLERFMRLREEALGDSSAYFHRFAELSVEDAREAALRVWDE
                     IDHPNLKENIEPTRDRARLILEKGRDHSVQSVRLRKI"
     gene            1663..2868
                     /locus_tag="VN178_01475"
     CDS             1663..2868
                     /locus_tag="VN178_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR05002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat
                     NxxGxxAF-containing protein"
                     /protein_id="HWS79663.1"
                     /translation="MVTHRRPSALRTLTLMFALLAALCLVLGAVLSDARAQTAPQYTF
                     TKVADSVEDGFVSFGCAAINNRGDIAFGAERLASDGFNTDPGIYRVNAADGALTTIAE
                     DPKRFVTIGLNPSINDSGHVSFAARLDGGKKPDTEVILRGGGRKLTTIATTADRFNFF
                     GFDTSINNSGEVAFTAELDEEFGFDEGLFSGSGGKRGVTTHYLTSTSQFDGRSSRPSI
                     NDSGNIAFVESVDFASGIFVGREGDFTPIVPPDSSDPSIGLGVPILNNDGTVAFQRSF
                     SDEATQEFVEEIVKIDAAGTSTVVADTRGEFATFGFRPPSLNNNGDVAFLATLDDFST
                     TGIFVGPDPSNDRVISTGDMLDGSTVQNITFCEEGLSDTGELAFVAQLEADTPDGFRT
                     AVFRATPVP"
     gene            3078..3287
                     /locus_tag="VN178_01480"
     CDS             3078..3287
                     /locus_tag="VN178_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79664.1"
                     /translation="MLRTILITGSIGMVVGFAGTLLTLFGGGSIRVEYVEFAVALLLF
                     LVMLIGAYWFVIGEPGIREGKKRKK"
     gene            complement(3444..4199)
                     /locus_tag="VN178_01485"
     CDS             complement(3444..4199)
                     /locus_tag="VN178_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79665.1"
                     /translation="MQTARNIDKTARTAREMAEVQRDSYEALAKNFAAAQRRTIGLAE
                     DGLEFMRLQEQSARAAQEWFAGGVRLLQLQQRNVEFVQGWTGNAVDAWREQTEHNVRT
                     AEAFARSASKQQEGLRTLAQAWAGTYRDFFSPFTYFQEGLRTGQQAARQGLQATEQVA
                     RQGLRIAEEATEQTDEVLRQTEKATKEAELRTAVSGALQTADYDELSVAEISKRLDGL
                     TTEQLRKVREYEKQNKNRETLIEQIDRKIRTNS"
     gene            complement(4470..4760)
                     /locus_tag="VN178_01490"
     CDS             complement(4470..4760)
                     /locus_tag="VN178_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79666.1"
                     /translation="MYVAVRRYEGITDSQKVAQLVEEDYVPVISQMPGFVAYYCVDAG
                     DGVMVSTSVFEHKDAEEQSTFRAGEFVAQDLGHLSPNPPQVTAGEVVAYKTK"
     gene            complement(4888..6327)
                     /locus_tag="VN178_01495"
     CDS             complement(4888..6327)
                     /locus_tag="VN178_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWS79667.1"
                     /translation="MAQQSSAGPNHLLIITDDQPHYMQNAKITPHIAARIANHGLKWV
                     QGSWANIPVCGPARATILTGKLAHNHGVWTNASYEGGGIHTAFKDLEGDTIATRSQAM
                     GVNTAIFGKYMNGYPGYVKNSDGSFDATRAAPPPGWDRFVIILAEDGDSTWLVNRHGE
                     EWRSEWPRTDTALIRSELVDWVTAPEQLSAPWLALMTPQSPHDPYTASPEHAHDFDGV
                     QLPNWRSYNEADMSDKPRELRAQPNITDPGGLAHGELRDWYEGKLEEIRDVDDAVNET
                     LTALADSGQLANTYVWFTSDNGYMFGQHRLAKKEQPYEESARVPFIVRGPGVPQHAFV
                     GRTSLAGPWIGHIDIPRTILSTFGANLSGTDGRDLRHVFRVGGAKPSGWRNRMLLEMN
                     TEFAGGQVAPAGSRIWQAMVRQKNARFKAIRRPPWGPSWEFYNLDSDLAEVSSVTSTM
                     NSTLKQQLKDELLAALTLSGDAYRSWEAS"
     gene            complement(6641..7153)
                     /locus_tag="VN178_01500"
     CDS             complement(6641..7153)
                     /locus_tag="VN178_01500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS79668.1"
                     /translation="MRMRDATLEDLASIVEIYNSIIPGRIVSADTEPVSVEQRLPWFH
                     EHHPARRPIRVAEEDGEILGWLSLGDFWDGRPAYDGTAEIGIYIKEGHRGKGLGGRML
                     EEAIGRAPDLGIKTLTAGAFAGNELSLKLFERFGFEKWAHFPRVAELDGNEQDLVVLG
                     LRLETKTASP"
     gene            complement(7156..8334)
                     /gene="dapC"
                     /locus_tag="VN178_01505"
     CDS             complement(7156..8334)
                     /gene="dapC"
                     /locus_tag="VN178_01505"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HWS79669.1"
                     /translation="MPNPRATPRTQKPPLLLNPILARQGAYPLLRLDERRWELERKGL
                     DLFDFGTGDPREPTDEKIRHALIEGVPEVSRYPSTVGKQDLREAFCGWMERRHGVTLD
                     PESEVLPATGSKEAIFHAPLAFLHHSHERRLVAYGTPGYPVYERGALLSGGEALPLEL
                     KWEDGFLLQPESIDPERTRILWINYPHNPTGATATYGYLERVVEFCSEHDILLFSDEC
                     YNDIYSGESPPSVLEITTERTLAFCSLSKRSGMTGYRSAMMAGDPELIAALGKLRPSI
                     GVATQTFVQDAATAAWDDDAHVEKRRRVFGEKRGLFTAFFERAGLEYLPTAASFYLWV
                     AIPGGDDEAYALRLMEEGVIVAPGRAFGAGGEGYIRIALVPGLEDCKLAIQRWAGIEG
                     "
     gene            8410..9657
                     /gene="lysA"
                     /locus_tag="VN178_01510"
     CDS             8410..9657
                     /gene="lysA"
                     /locus_tag="VN178_01510"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWS79670.1"
                     /translation="MDDFHYEGGDLRCEGVPLSEIAAAGTPTYVYSHAALERAYTELD
                     SAFAGLDHLVCYAVKANGNLAVLRALASLGAGADIVSGGELYRVMRAGFDPKKVVFAG
                     VGKTGQELMSGLGERILLFNVESASELEHLDRLCSRFGKRARVALRVNPDVDAETHDH
                     VATGRGRDKFGIPVDEALALAERVGGYRCIDLIGVHQHIGSQITKLAPYTESVERSAG
                     LVDELKTRGFDIRYFNIGGGLGIPYKDEETPTPKELVDAIRPMLEAAGTKILCEMGRY
                     IAGAAGVLMTKVVYRKKSGEKSFIVVDAGMNDLLRPSLYDAHHEVLSLNEDGSVANAD
                     LVGPVCESGDYLARDRELPDASEGDVLAIMDAGAYGFSMASNYNSRPRPAEVMVKGES
                     WAVVREREHSADLVKGELVPAFL"
     gene            9703..10470
                     /locus_tag="VN178_01515"
     CDS             9703..10470
                     /locus_tag="VN178_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79671.1"
                     /translation="MNVPDGLEVFTLAERPDLESRVGSLPDMFPEFMHHDSTVSRYWG
                     GLFTDFAGFQIVVCDRGDEVVAAGHCIPVFWDETDEGLPAGLDGVLEQGVRDFEVGRA
                     PTVVSALLAIVSHAHRGRGLSSVVLTTMKSVAADHGLGALIAPVRPTLKERYPLTPME
                     RYFRWERPDGLPFDPWFRVHRRLGAEFVRVAPRSMLITGTISEWEEWTGMRFPESDAY
                     VVPGALQPVVMDLERDLGTYEEPNVWMRHPIAEGRSC"
     gene            10464..11009
                     /locus_tag="VN178_01520"
     CDS             10464..11009
                     /locus_tag="VN178_01520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWS79672.1"
                     /translation="MLRGEKVTLRAIEREDLERLWRFWNDLEVELAGGGDPPLPVSLQ
                     RLRERFDREAREGTPDKTNFAIEADGECIGQCGLFHFDVASRHCELGIAIGEKEYWGR
                     GYGREAVRLILDYAFRIRNFRRVWLETHAANERAIRAYKACGFVEEGRMREHIWLAGR
                     YVDNVIMGIVREEWREGRTVG"
     gene            11002..11922
                     /locus_tag="VN178_01525"
     CDS             11002..11922
                     /locus_tag="VN178_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWS79673.1"
                     /translation="MARGTHEALPDERNEDVLVYVNGEFYRREEAKISVFDSGFLVGD
                     GVWEGIRLHGGEFAFLDLHLDRLLEGAKTIGLDIGMTREEITAALRETVRRNGMEGST
                     GVHVRLMVTRGDKKTPSQDPRLVVGGPNVVIIAEHKVADPGVKEWGLRLFTSTVRRPP
                     PDTLDQRLNCHSKLHEVVALLQALEAGADEALMLDPTGAVATCNATNFFVVRRGEVWT
                     STGHYSLNGITRRMIIDLCQASGVPVFERTFSLTDVYGADEAFVTGTFGGITPVVEVD
                     GRKIGTGQRGDVTARLTDLYAEAVAEATGG"
     gene            11925..>12404
                     /locus_tag="VN178_01530"
     CDS             11925..>12404
                     /locus_tag="VN178_01530"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79674.1"
                     /translation="MPGCLRLSVWSGPRNVSTALMYSFRQRADTVVVDEPLYGHYLKT
                     TGADHPGAREVMEVMDGDGERVVREVILGPCERRIRFFKNMAHHLPGLDPGFLDSVTN
                     VLLIRNPTEMLPSLAKQLSSPILRDTGLREQAGILDSILASGGEPIIIDVRELLLDPP
                     "
BASE COUNT         2192 a         3840 c         3953 g         2419 t
ORIGIN      
        1 acccaggcac cggcgacaac gagatctatt acatcaacac caacgggggt ggtaatactc
       61 gagtcaccga caacaatacg cagaactggg atccttactg ggcatgtaaa ggagcttgta
      121 ggtcgaggta gcgattgctc cctccgggta gggccggagg tgtgagtttc gcacagtaaa
      181 gaactcagga gcccggaggc gccatggcgc gtttgccggg ctcctcttgg ttcgagcttg
      241 ctccgtctcc tcgtgtagcc tctcctcagc acgtgaaaac ctcacccatt tgcgctagag
      301 aaaaatcact catttgcggt atgtgctcca gggcacctcg gtactataat tcaggtaatc
      361 gccaagccgg gccgcactct gaacccttcc ccctccaaag cgcgacccgg ctttcctctt
      421 gcattagccg tcagctttca gcgatcagcg gtcagctaga aaagctgaaa gctgagtgct
      481 gacagcgcag ttttggaagg gaaaccttcc aaaaccgcta tcagatcttc ctcaagcgta
      541 cgctttggac ggagtgatct cgacccttct cgaggatcag gcgcgccctg tctctggtcg
      601 gctcgatgtt ttctttgagg ttggggtggt cgatctcgtc ccagacgcgt agggctgctt
      661 ctctggcgtc ctcgacggag agctccgcga aacggtggaa gtaggcggag ctatccccga
      721 gcgcctcttc ccgcaggcgc atgaagcgct ccagatacca ttctttgatg tgcctctcct
      781 gcgcgtcgac gtagatagtg aagtcgcagt agtcggagac gaagaccctg tgtccttcgg
      841 agggctcagg gagtccggcc tccagcatgt tgaggccttc cacgatgagg acatcgggac
      901 gtccgaggat ctgggtcctg tcggggagga tgtcgtaggt caggtgagag tggatcgggg
      961 cgctcacccc gtcgcgaccg gatttgatgt ctgcgaggaa cctcagtagg agcggcaggt
     1021 cgtagctctc ggggaagccc ttgcgttcca tcaacccccg agatctcagc acgctgttgg
     1081 ggtagagaaa gccgtctgtg ctaaccaacc caacctcggg acgccccggc cagcgggaga
     1141 tcagggctcg cagtaccctg gccaccgtgc tcttgccggc ggcgacgctc cccgcgatgc
     1201 cgatgacgaa gggcacctct ttctcttctt cggccaggaa cgcgcgtgct gcccggtaca
     1261 gactctgcgt ggcgcttaca tggaggtaaa ggagccgcga gagggggagg tagacttctc
     1321 tagcttcctc cagcgagagg tacgccccta tctcccgcgc atcgtcttcg gaaagagcga
     1381 acgaggcgtc tgccggcaac ccggcccaat cctcgcggga gagacgcaca aacgggtgga
     1441 gactgttctg cccggcgtcc acggtggcgt cctcctctca ccctctctac agagtccctg
     1501 tgacagtctt gcacaaactc ttctggggcg cccctatatc ccatccattc gggttgctag
     1561 tgcgtataca cgggcagcct tcggcgcttg ccggagcaaa ggagggagag tgaccgccgg
     1621 cagcacgacc ccgtgcgagc gtggtctaca gaaggaggaa gtatggtgac ccacagacga
     1681 ccgtccgccc tgcgcacttt gacgctgatg ttcgctctcc tggcagcact gtgcctggtg
     1741 ctcggggcgg tgctatctga tgcgcgggcg cagactgccc ctcagtacac attcacaaag
     1801 gtggctgaca gcgtagagga cggcttcgtt agttttggct gcgctgcgat caacaaccga
     1861 ggcgatatcg cgttcggggc ggaacgcctg gcctcagacg ggttcaatac agatcccggc
     1921 atataccggg tgaacgcagc ggacggagca ctgacgacca tcgccgaaga cccgaagcga
     1981 ttcgttacca tcggactcaa cccctcgata aacgactcgg gccatgtctc gttcgcggcc
     2041 agactcgacg gcggtaagaa gccagacacc gaggtcatct tgcggggcgg cggcaggaag
     2101 cttactacca tcgcgacgac ggcggaccgt ttcaactttt tcggtttcga cacctccatc
     2161 aacaacagtg gcgaggtggc cttcacggcc gaactcgacg aggaattcgg cttcgatgag
     2221 ggcctgttct caggaagcgg cggcaagcga ggcgtcacga cgcactatct cacctcgacc
     2281 agccagttcg acggcagaag ttcgaggccc tcgatcaacg attccggcaa catagccttc
     2341 gtcgagtcgg tcgacttcgc cagtggtatc ttcgtgggta gggaaggaga ctttacaccg
     2401 atagttccgc cagactcttc cgacccttct atcggactcg gagtaccgat cctgaacaac
     2461 gacggaaccg tagccttcca gaggtccttt agcgacgagg ctacccagga gttcgtcgag
     2521 gagatcgtaa agatcgacgc tgccggtaca tccacagtgg tcgccgacac gaggggtgag
     2581 ttcgccactt tcggcttcag gccgccctcg ctgaacaaca acggcgacgt ggccttcctc
     2641 gccaccctcg acgacttttc gactaccggg atcttcgtcg ggccagaccc cagtaacgat
     2701 cgggtgatct ccacgggaga catgctggac ggctcgacgg ttcaaaacat caccttctgc
     2761 gaagagggcc tcagcgacac tggcgagctg gcgttcgttg cgcaattgga ggccgatacg
     2821 ccggacggct tccgcacggc cgtcttccgc gccacgccgg tgccctagcg aaaggaacgt
     2881 ctaaggtaag ccatcagctt tcagctatca gctttttgtt ttggctgaaa gctgaaagct
     2941 cccttctcgg aaaggttcaa cctttccgag aagggctcta atagtatctc gcccccggcg
     3001 catgatttgg ggtaggctat cgtggtggat cgtgaaggct tgaagcggtg aacccactta
     3061 gactgccagg ggagacattg ctgagaacga tactcataac gggttcgata gggatggtcg
     3121 tcggctttgc cggcacgctg ttgactttgt tcgggggcgg gagcatcagg gtggagtatg
     3181 tggagttcgc cgtggcgctg ctcctgtttt tggttatgct gatcggtgcg tactggttcg
     3241 tgataggcga gcctggcata agggaaggga agaaacgaaa gaagtaacgc cagagccttt
     3301 cacgggaagg ttctaccttc ccgtgaaagg agccatcagc cgtcagcgat cagcactttg
     3361 ttttggctga aagctgatcg ctgagggctg acagcgcgat ttgccgaagg ttcgaccttc
     3421 ggcaaatcgc agtagagctc gccttacgag ttggtcctta tcttgcggtc gatctgctcg
     3481 atcagggtct cgcggttctt gttctgcttc tcgtactcgc ggaccttacg gagctgctcg
     3541 gtggtgaggc cgtccagcct cttggagatc tccgcgacgg acaattcgtc gtagtcggcg
     3601 gtctgcaggg cgccagagac ggcggttctc agctccgcct cctttgtggc cttctcggtc
     3661 tggcgaagga cctcatcggt ctgctcggtg gcctcctcag cgatcctgag cccctgcctt
     3721 gccacctgct cggtggcctg aagtccctgc cgggcggcct gctggcccgt ccgcaggccc
     3781 tcctgaaagt aggtgaaggg ggagaagaag tccctgtagg tccccgccca agcctgggcg
     3841 agcgtcctca agccctcctg ctgcttgctc gcgctccgcg cgaaggcctc ggcggtgcgc
     3901 acgttgtgct cggtctgctc gcgccacgcg tccacggcgt tgcccgtcca gccctgcacg
     3961 aactcgacgt tgcgctgctg gagctgcaac agcctcacgc caccagcgaa ccactcctgg
     4021 gccgcgcggg cgctctgctc ctgcaatctc atgaactcga gcccgtcttc ggcgagcccg
     4081 atggtgcggc gctgggcagc cgcgaagttc ttcgcgagag cctcgtaaga atcgcgctgg
     4141 acctccgcca tctcgcgggc ggtgcgcgcc gtcttgtcga tgttcctggc agtctgcacc
     4201 aatatacctc catggtagaa gatcttgacg ctatattcct accacacata tattcgattg
     4261 caaaccctcg caacatccgg ccttctcacg gcgggttgca ggaggtcaat cctcctgcaa
     4321 cccgcgcggt cagccctcaa ccctcagccc tcagcttttc gttcttgctg atcgctgaaa
     4381 gctgatcgct gaaagctcct cgtgcataag ggttgaaccc cttatgcacg gcgcagtcac
     4441 atctagctct cgaattctct gcgtctcgcc tacttcgtct tgtatgcgac gacctcaccg
     4501 gccgtgactt ggggcgggtt cggggacaaa tgacccagat cctgcgccac gaactctccc
     4561 gccctgaagg tcgactgttc ctcggcgtcc ttgtgctcga agacgctggt cgaaaccatc
     4621 acgccgtccc cggcgtcgac gcagtagtaa gccacgaatc ccggcatttg gctgatgacg
     4681 ggcacgtagt cctcctcgac cagctgcgcc accttttgcg agtcggttat gccctcgtag
     4741 cgtcgcactg caacgtacat agccctggcc tacctccttc tcgtggaggg cggggtgata
     4801 ggccccgcct atggtcgtac aactctaggc acgctaccaa acatcggcgc caggcgcatc
     4861 actcggatga gtgatctacc tcatacccta gcttgcctcc caactacggt aggcgtcacc
     4921 cgagagtgtg agagccgcaa gaagctcgtc cttgagctgc tgcttgaggg tggagttcat
     4981 ggtcgaggtg acagagctga cctccgccag gtcactgtcg aggttataga actcccagct
     5041 cggtccccat ggggggcgcc tgatggcctt gaagcgggca ttcttttgcc ggaccattgc
     5101 ttgccagatg cgacttccag ccggggccac ctgtccgcct gcgaactcgg tgttcatctc
     5161 caggagcatc ctgttgcgcc agccactagg tttggctccg ccgactcgga agacgtggcg
     5221 caggtcgcgt ccgtcggtgc cggagaggtt cgcgccaaag gtagagagga tggtgcgcgg
     5281 gatgtcgatg tggccgatcc aggggcccgc aaggctcgtc cggccgacga aggcgtgttg
     5341 cggtacgccg ggaccgcgta cgatgaacgg cacccgggcg gactcttcgt agggttgctc
     5401 cttcttcgcc aacctgtgct gtccgaacat atacccatta tccgaagtga accagacgta
     5461 ggtgtttgcg agctgacccg aatccgcgag agccgtaagg gtttcgttca cggcgtcatc
     5521 cacgtcacga atctcttcca gcttgccctc ataccagtcc ctgagctcgc catgcgccag
     5581 gccgccgggg tccgtgatgt tcggttgagc cctgagttct cggggcttgt ccgacatgtc
     5641 ggcttcgttg taactgcgcc agttggggag ctgcacccca tcgaagtcgt gtgcatgctc
     5701 gggggaggcg gtgtatgggt cgtgcgggct ctggggtgtc ataagcgcga gccacggagc
     5761 ggagagttgc tcgggggccg tcacccagtc tacgagttcg ctccttatga gcgccgtgtc
     5821 ggttctgggc cactctgaac gccactcttc accgtggcgg ttgaccaacc acgtagagtc
     5881 cccgtcttcc gcgaggatga tgacaaacct gtcccaaccc ggagggggcg ctgcgcgggt
     5941 ggcgtcgaaa gagccgtcgg agttcttcac gtagcccgga tatccgttca tgtacttccc
     6001 aaagattgcg gtgttgaccc ccatagcctg cgagcgcgtc gctatcgtgt cgccctcgag
     6061 gtctttgaag gcggtgtgga tgccaccccc ctcgtaactc gcgttggtcc acactccgtg
     6121 gttgtgagcc agcttgccgg tcagtatcgt tgcgcgcgca ggtccgcaga cgggtatgtt
     6181 tgcccacgaa ccctgcaccc acttcaggcc gtggttcgcg attctcgctg ctatgtgcgg
     6241 cgtgatcttc gcgttctgca tgtagtgggg ctggtcgtcg gtgatgatga gtagatgatt
     6301 gggccccgcg ctcgactgtt gagccatact ctccttttcg agcggcaggg tttgcccagc
     6361 cgccctcggt ttgttttgcg gttaccagtt tctcaccttc ttacagcgcc gtgcaaaagg
     6421 gttcaaccct tatgcacggc gagctttcag caagaacaaa aagctgaaag ctgaaagctg
     6481 atagctgacg gcgcgggttg cagaaggtca atccttctgc aacccgctgt tagaactgcg
     6541 tacaccggaa gtgcgcagaa tgcaccaggc tgctgaaaaa cgatctcggt cgtagaagcc
     6601 gttgcgggtt agacgccctg atctccgaat ctcgacgggg tcaggggctc gctgttttcg
     6661 tctcgagtct gagtccgagg acgacgaggt cttgctcgtt gccgtcgagt tcggcgacgc
     6721 gggggaagtg cgcccacttt tcgaagccga agcgttcgaa gagtttgagg ctgagttcgt
     6781 tgccggcgaa ggcccccgcg gtcagggtct ttatgcccag atccggagcc cgcccgatgg
     6841 cctcttccag catgcgcccg cccagtccct tacccctgtg gccctccttg atgtagatgc
     6901 cgatctcggc ggtcccgtcg taggccggcc tgccgtccca gaagtcgccg aggcttagcc
     6961 agcccaatat ctctccatcc tcttcggcga cccggatcgg acgtctcgcc gggtggtgct
     7021 cgtggaacca cggcaacctc tgttctacgg agaccggctc cgtgtcggcc gacactatcc
     7081 ggccagggat tatggagttg tagatctcca cgatggaggc gagatcctcc agcgtggcgt
     7141 cacgcattct catgctcacc cttcgatccc cgcccagcgc tgtatcgcca gcttgcaatc
     7201 ctcgaggccg ggtacaagcg cgatccttat gtacccttcc ccgcctgctc cgaaggccct
     7261 gccgggcgcc acgatcactc cctcctccat cagccgcagc gcgtaggcct cgtcgtctcc
     7321 accaggtatc gcgacccaca ggtagaagct cgctgcggtg ggcagatatt caagtccggc
     7381 tctctcgaag aacgcggtga agagcccccg cttctcgccg aagacgcgcc gccgcttctc
     7441 gacgtgggcg tcgtcgtccc aggccgcggt cgcggcgtcc tgcacgaagg tctgggtggc
     7501 cacgccgatg gagggacgga gcttacccag cgcggcgatg agctcggggt ccccggccat
     7561 catggccgag cggtagcccg tcatcccgct gcgcttggag agggagcaga aggcgagggt
     7621 ccgctccgtg gtgatctcca ggacggaagg cggggactcg ccggagtaga tgtcgttgta
     7681 gcattcgtcg gagaagagca ggatgtcgtg ctcgctgcag aactcgacca ccctttccag
     7741 gtacccgtac gttgccgtcg cgcccgtggg gttgtggggg tagttgatcc agagtatccg
     7801 cgtgcgttcg gggtcgatcg actcgggctg cagcaggaaa ccgtcctccc atttcagctc
     7861 aaggggcagg gcctcgccgc cggagagcag cgcgccccgt tcgtagacgg ggtagccggg
     7921 cgtgccgtag gcgacgaggc gccgctcgtg cgagtggtgc aggaaggcca gaggagcgtg
     7981 gaagatggcc tcctttgagc ccgtggccgg caagacctcg ctctcggggt cgagcgtgac
     8041 cccgtggcgg cgttccatcc agccgcagaa ggcttcgcgg agatcctgct tgcccaccgt
     8101 actcgggtac ctgctgactt cagggacgcc ctctatgagg gcgtggcgga tcttttcgtc
     8161 cgtcggctcg cgcggatcgc cggtgccgaa gtcgaagaga tccaagccct tcctctccag
     8221 ctcccagcgg cgttcgtcga ggcgcagaag cgggtaggcg ccttgcctgg cgagtatggg
     8281 gttgaggagt aaggggggct tttgcgtccg gggtgtcgcg cgggggttcg gcacggagta
     8341 tattctccct acggctccaa cggcagagcc agaaatatag cacgccccgg ctacacgagg
     8401 aggtacgttt tggacgactt tcactacgag gggggcgatc tccgctgcga gggtgtgccg
     8461 ctctccgaga tagccgcggc gggaaccccg acctacgtct acagccacgc cgcgctggag
     8521 agggcttaca cggagctaga cagcgcgttt gcgggcctcg atcacctggt ctgctacgcg
     8581 gtcaaggcga acgggaacct cgccgtgttg cgggcgctcg cgtcactggg ggcgggagcg
     8641 gacatcgtct ccggcgggga gctctacagg gtgatgcggg ccgggttcga cccgaagaag
     8701 gtcgtcttcg ccggggtcgg gaagaccggg caggagctca tgtctgggct cggggagcgc
     8761 atcctgctct tcaacgtcga gtcggcctcc gagctcgagc acctcgaccg cctctgttcc
     8821 cgcttcggca agagggccag ggtggcgctg cgcgtcaacc ccgacgtcga cgccgaaacc
     8881 cacgaccacg tcgcgacggg gaggggccgc gacaagttcg gcatccccgt ggacgaggcg
     8941 ctggctctgg ccgagagagt agggggttac cggtgcatcg acctcatcgg ggtccaccag
     9001 cacatcgggt cccagatcac caagctcgca ccttacaccg agtccgtcga acgctccgcc
     9061 ggcctagtcg acgaactcaa gacccgcggc ttcgacatcc gctacttcaa catcggcggc
     9121 ggcctcggca ttccttacaa ggacgaagag accccgaccc cgaaagagct cgtggacgcg
     9181 atcaggccga tgctcgaggc ggcggggacg aagatcctgt gcgagatggg ccgctacata
     9241 gcgggcgccg ccggggtgct catgacgaag gtcgtctacc gcaagaagag cggcgagaag
     9301 agctttatcg tagtcgacgc cggtatgaac gacctcctgc gtcccagcct ctacgacgcc
     9361 caccacgagg tcctctcctt aaacgaggac ggctcagtgg cgaacgccga cctcgtcggg
     9421 cccgtttgcg agtccggcga ctacctcgcc cgcgaccgcg agttgccgga cgcctccgag
     9481 ggcgacgtgc tcgccatcat ggacgccggc gcctacgggt tctcgatggc ctccaactac
     9541 aactcccgcc ccaggcccgc ggaggtcatg gtcaagggag aaagctgggc cgtggtgcgc
     9601 gaacgcgagc acagcgccga cctcgtgaaa ggcgagctgg ttccggcgtt cttgtaggtt
     9661 ttataggttt tagatgtcag gtctgcgtgg tcggtggctc ttgtgaacgt acccgacggg
     9721 ctggaggtct tcacgctcgc cgagaggccg gatctcgagt cgcgggtggg ctccctgccc
     9781 gatatgttcc ccgagttcat gcaccacgat tcgacggtca gccgctactg gggaggcttg
     9841 tttaccgatt tcgccggatt ccagatcgtc gtctgcgacc gtggggacga agtcgtcgcg
     9901 gcaggccatt gcatccccgt tttctgggat gagactgacg aagggttgcc ggcaggcctg
     9961 gatggggtgc tcgagcaggg ggtgagggat ttcgaggtgg gacgagcgcc aacggtggtc
    10021 tcggccctcc tggcgatcgt atcgcacgcc caccggggac gtggcctcag cagcgtggta
    10081 ctcaccacga tgaagtccgt cgcggcggat cacggactgg gagccctgat cgcgcccgtc
    10141 cgtcccacgc tcaaggagcg gtatccgctc accccgatgg agcgctactt ccggtgggag
    10201 cgccccgacg gactaccctt cgatccctgg ttcagggtcc accggaggct cggcgcggag
    10261 ttcgtacggg tcgcaccccg ttccatgctt atcacgggaa cgatctccga gtgggaggag
    10321 tggacaggca tgcgttttcc cgagagcgac gcctacgtcg tgcctggagc cctgcaaccc
    10381 gtcgtgatgg atctcgaacg tgacctaggc acctacgaag agccgaacgt ctggatgcgc
    10441 caccctatcg cggagggtag atcatgctga ggggagagaa ggttacgctg cgggccatag
    10501 agcgcgagga tctggagcgc ctgtggcggt tctggaacga tctcgaggtc gagctcgcgg
    10561 gtggcggcga cccgccgctg cccgtctccc tccaacggct gagggagagg ttcgatcgcg
    10621 aagcccggga aggaactccg gacaagacga acttcgccat cgaggccgac ggcgagtgca
    10681 tcgggcagtg cggcctcttc cacttcgacg tggcttcccg ccactgtgaa ctgggcatcg
    10741 ctatcggcga aaaggaatac tggggccgcg gctacgggcg cgaagccgtg aggttgatcc
    10801 tcgattacgc gtttcggata aggaacttca ggcgcgtgtg gctggagacc cacgcggcga
    10861 acgagcgtgc gatccgggcc tacaaggcct gcggcttcgt cgaggagggc aggatgcgcg
    10921 agcacatctg gctcgccgga cgctacgtgg acaacgtcat catgggcatc gtgcgcgagg
    10981 agtggcgtga agggaggacg gttggctagg gggacgcacg aggcattgcc cgacgagcgc
    11041 aacgaggatg tcctcgtcta cgtcaacggc gagttctacc gccgggagga agcgaagatc
    11101 tccgtcttcg actcgggctt cctggtcggg gacggggtgt gggagggtat tcgcctgcac
    11161 ggtggcgagt tcgcgttcct ggatctacac ctcgaccgtc tccttgaggg tgcgaagacg
    11221 atagggctcg acatcggtat gacgcgcgag gagatcaccg cggccctccg cgagaccgtc
    11281 cggcgcaacg gcatggaagg ctcaaccggc gtccacgtgc gcctgatggt aacccgcggg
    11341 gacaagaaga ccccctcgca ggacccgcgt ctcgtggtcg gcggtccgaa cgtcgtcatc
    11401 atcgccgagc acaaggtggc cgaccctggc gtcaaagagt ggggcctcag gctcttcacc
    11461 tcgacggtgc gccgcccgcc gccggatact ttggatcagc ggctcaactg ccacagcaag
    11521 ctgcacgagg tggtggccct actacaggcc ctcgaggcgg gcgccgacga ggctctgatg
    11581 ctcgatccta ccggggcggt ggcgacctgc aacgccacca acttcttcgt cgtgcggcgc
    11641 ggagaggtct ggacctcgac aggtcactac tccctgaacg gcatcacccg gcggatgatc
    11701 atagacctct gccaggcctc gggagtcccc gtcttcgagc ggaccttctc gctcaccgac
    11761 gtgtacggcg ctgacgaggc gttcgtcacg ggtaccttcg gcggcataac accggtcgtc
    11821 gaggtggacg gacgcaagat cggcaccggc cagcgcggtg acgttaccgc ccgccttacg
    11881 gacctgtacg cggaggcggt cgccgaggct accggcgggt agcggtgccg ggatgcctca
    11941 ggctgtcggt gtggtcgggg ccgcgcaacg tgtcgacggc gctcatgtac tccttcaggc
    12001 agcgcgcgga taccgtggtg gtggacgagc ctctctacgg acattacctg aagaccacag
    12061 gcgcggatca tcccggtgcc agggaggtca tggaggtgat ggacggggac ggcgaacggg
    12121 tggtgcggga ggtcatcctc ggtccgtgcg agcgccggat ccgcttcttc aagaacatgg
    12181 cgcaccatct tcccggcctc gaccccggtt tcctggacag cgttaccaac gtgctcctga
    12241 tccggaaccc caccgagatg ttgccctcgt tggccaaaca actctcctcc ccgatcctgc
    12301 gggacacggg cctgcgcgag caggccggga tcctcgactc gatcctggcc agcggcgggg
    12361 aacccatcat catcgacgtc cgcgagctac tcctcgaccc gccg
//
LOCUS       DATMVF010000037         7096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_3555, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000037 DATMVF010000000
VERSION     DATMVF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VN178_01535"
     CDS             <1..745
                     /locus_tag="VN178_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HWS79675.1"
                     /translation="DEDHLLISARRTSAVYKVDKRTGEVVWRLGGEKSDFEMGEGVRT
                     ALQHDARRNADGTITIFDNGNVERVDQSRAIMVEVDEDKMSASLVREYTHPDKLLSDT
                     QGNVQVLPNGNVLVGWGSAPYFSEFDHHGELLFHAAFPADGETYRAFRLPWIGQPNDY
                     PAIAAESGPEDKVTIYASWNGATEVTTWHVLAGSAPDRLEPLASAPHQGFETVITVHT
                     TEPYVGLSAVNGSGEVLGTTNAFRLENNT"
     gene            complement(835..1173)
                     /locus_tag="VN178_01540"
     CDS             complement(835..1173)
                     /locus_tag="VN178_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79676.1"
                     /translation="MEGLDPAEFQEISAMISDIQRERAMARQLKREQRRSAARKKAQN
                     KRYLELEHVKCGKKGCNKCAGGELHGPYWYLYRPKHNGLGRTSEYVGKLLPEALAEEF
                     EIPEDRRTAN"
     gene            complement(1374..1784)
                     /locus_tag="VN178_01545"
     CDS             complement(1374..1784)
                     /locus_tag="VN178_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS79677.1"
                     /translation="MDEQRVPDLEELDEKQVRRFLAELFSDLSAAAEFNVRHEDYVME
                     MPQSGERIRGRDKMREFQEDYPSPPTIRLRRVVVREGLWVIEGVNDYGGGRVFSVVAI
                     FELRDGKIWRDTRYYAEPFEAPQWRAQLVERMES"
     gene            complement(1819..2526)
                     /locus_tag="VN178_01550"
     CDS             complement(1819..2526)
                     /locus_tag="VN178_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HWS79678.1"
                     /translation="MTETNLEQFREVTPEGRFTLQNSAMLKVKLENDQIQAKLGSMVA
                     YQGDVSFEHKSGGLGRFFKKAVTGEGVKLMAANGTGNLFLAMDKRKIMILDLHNERMT
                     VNGDNILAFEPGIEWDIERVEGAGRLAGGLFNVVLEGSGKVAVTSDGEPVLLDTSTPT
                     FADPESAIAWSGGVRTGVRSDVSFKTFTGRGSGESFQVSFEGAGWVLIQPSEGGVVPE
                     HSHAGDGGGGLGSLFED"
     gene            complement(2595..2933)
                     /locus_tag="VN178_01555"
     CDS             complement(2595..2933)
                     /locus_tag="VN178_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HWS79679.1"
                     /translation="MPEIGWDDFEKVDVRVGTVIGADPFPEARKPSIRLVVDFGPEVG
                     TRKTSAQLTANYEPEVLVGRQVLAVINFPPKRIAGFESEVLVLGVPDENDEVVLLAPD
                     LRVPPGGRMF"
     gene            complement(3037..3765)
                     /locus_tag="VN178_01560"
     CDS             complement(3037..3765)
                     /locus_tag="VN178_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWS79680.1"
                     /translation="MEQRIEDLLLQGRYRAGERIPPETGLVDALGVSRVTVRAGLARL
                     VERGLLERRQGSGTFLVRPPEGARLSAGLERLETYTVHAARLGLELGSSGLTIEPVGA
                     SPDEAAALEVAEGTPLARVSRALLVEGNPAAWMVDVVLQSIIGVDEVKERFRPDAMLL
                     DLLVSEGVPVGYSQMSIGAVMIGPKDPTGERLGLRVDSAALSLIQTMYLTDGHPAQWS
                     RDTFLPGKLNLHVVRELFEVRNLP"
     gene            4016..5485
                     /gene="eat"
                     /locus_tag="VN178_01565"
     CDS             4016..5485
                     /gene="eat"
                     /locus_tag="VN178_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HWS79681.1"
                     /translation="MSEQERRTRQGAGYENVGSDYLEQRQLQKGAAGWVLLAGLGVAY
                     VISGDFAGWHFGLGAGGWGGLLIATVLMATMYTAMVFALAELSSTIPTAGGGYGFARR
                     ALGPWGGFMTGTAILIEYAIAPAAIATFIGGYVETLTGIGGWIVYLVCYLIFVGIHLY
                     GVGEALKVTFGITVVAVVALAAFVIGMIPQFEVANLFDIDPTGAAGASSFLPFGVVGI
                     WAAFPFAIWFFLAIEGVPLAAEETRDPATDMPKGLIAGMAALLLFAVLVLFFGPGGAG
                     SAAFTSGEIVLAPSVLPLAMEEAYGGGNALVTFVNIVGLAGLIASFFSLIFAYSRQMF
                     ALSRAGYLPRLLSVTGGRRTPWVALIVPGAIGLVLAIAGGSAGGATLLNSAVFGATIS
                     YALMMLSHIVLRQREPDLDRPYRTPGGVATSGVALVLAVLAVIATFIVDVRAALIMLG
                     VYALFLAYFGLYSRHHLVAQAPEEEFAAIEKAESELAGN"
     gene            5499..6863
                     /locus_tag="VN178_01570"
     CDS             5499..6863
                     /locus_tag="VN178_01570"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWS79682.1"
                     /translation="MDTTQAAGRLDLDTLRSEVEAGNIDTVLLAMTDMQGRLQGKRLT
                     ATHFLDEVVEHDAEGCNYLLAVDVEMNTVEGYAMSSWERGYGDFVFKPDMDTLRYVPW
                     QEGTAFVTCDLVWEDGSPVVASPRQVLKRQLGRLSERGLEAFVGTELEFIVFMDSYEE
                     AWRKGYRDLEPANYYNVDYSLLGTARIEPLLRRIRNSMAGAGLAVEDAKGECNFGQHE
                     INFRFDRALETADGHAIYKNGAKEIAAQEGCSISFMPKFDEREGNSCHIHLSLRREDG
                     APVFTDGHGFSPTFEHFIAGQLACLRELTLFFAPNINSYKRYAKGSFAPTAVAWGKDN
                     RTCSLRVVGHGNSLRVENRLPGGDVNPYLAIAAMIAAGLHGIENELSLEEEFIGNAYE
                     ADKPHVPTNLREARELFAASEAARSAFGEEVVEHYLNMARVEIEAFEAAVTDWERYRN
                     FERL"
     gene            6860..>7096
                     /locus_tag="VN178_01575"
     CDS             6860..>7096
                     /locus_tag="VN178_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566105.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWS79683.1"
                     /translation="MKAGSRPTIGVTAATERVSYGVWNEIPAVISPARYLQAVQRAGG
                     RPVLLPPDPEDAEDPGGVLDLLDALVVTGGAGDLD"
BASE COUNT         1297 a         2252 c         2194 g         1353 t
ORIGIN      
        1 tgacgaggat cacctgctca tctcggctcg caggacgagc gcggtctaca aggtggacaa
       61 aagaacgggg gaagtggtgt ggcgactggg tggggagaag agcgacttcg agatgggaga
      121 gggtgtccga accgcccttc agcatgacgc ccgtcgaaac gccgacggca cgataaccat
      181 cttcgataat ggcaacgtag agcgagtaga tcagtcccgc gccattatgg tcgaggtcga
      241 cgaagataaa atgagtgcca gcctggtgcg cgagtacacc catcccgaca agttgctctc
      301 tgatactcag ggcaacgtgc aggtattgcc gaacggcaac gttcttgtag gctggggcag
      361 cgcgccgtac ttctccgagt tcgatcatca tggcgaactg cttttccacg ccgccttccc
      421 cgccgacggc gagacctaca gggccttccg tctcccatgg atcgggcaac ctaacgacta
      481 ccctgccatc gccgccgagt cagggccgga agacaaggta accatctacg ccagctggaa
      541 cggcgctacg gaggtaacta cctggcacgt gttggccggt agcgccccag atcgactgga
      601 gcccctcgca tcagcccccc atcagggctt cgagaccgtg atcacggtac acaccaccga
      661 accctacgtc ggcctgtcag ccgttaacgg ctcgggtgag gtgctcggta ccaccaacgc
      721 attcaggctg gagaacaata cctagccctc gagctgcgac agccggtctc gtcgcccatc
      781 ttatgtagat gaagccgaaa gcgggcaatc tgctgtacat aagaaacggg tcggctagtt
      841 cgccgtccgc cggtcttcgg ggatttcgaa ctcttcggcg agtgcctcgg gcagcagctt
      901 gccgacatat tcgctggtac gtcctagtcc gttatgcttg ggtcggtaca ggtaccagta
      961 agggccgtga agctctccgc cggcgcattt attgcagccc ttcttaccgc atttgacgtg
     1021 ttccagttcg agatagcgct tgttttgggc ttttttgcgc gccgcggacc ggcgctgttc
     1081 gcgtttcagt tgtcgcgcca ttgcgcgctc acgttgtatg tcggagatca tggccgagat
     1141 ctcctggaac tccgccggat ccaacccctc caaccactcc ctcgttatgg gccgtggttc
     1201 catcacttcc tccagatcag ctccctatgt acatcctagc acgagtctgt acaaaatgta
     1261 cataagaaag agtaagggcg aagcgtctta cggttcggga ttcatcgaga tggcacgcat
     1321 tgccctggac aaacctgaag gggcggtttg cgaaccgccc ggatacgaac cgctcaagat
     1381 tccatccgtt cgaccaactg cgcccgccac tgtggcgcct cgaacggctc ggcgtagtag
     1441 cgggtgtcgc gccagatctt gccgtcgcgc agctcgaaga tggccacgac ggagaaaaca
     1501 cgtcccccgc cgtagtcatt gacgccctcg atgacccaca agccttctct gacgactact
     1561 cttcgcagcc ggatggttgg gggagagggg taatcctcct ggaactccct catcttatcg
     1621 cggcccctga tgcgttctcc ggattggggc atctccatta cgtagtcttc gtggcgcacg
     1681 ttgaattccg ctgcggcgct cagatcggag aaaagttcgg ctaggaagcg gcgcacctgt
     1741 ttctcgtcca actcttcgag atcgggcacc cgttgctcat ccatctggcc ttctcctgta
     1801 gcagttgcgg gcggccggct agtcctcgaa caaggagccc agaccaccgc cgccatcgcc
     1861 ggcgtgggaa tgctcgggaa cgacaccgcc ttccgagggt tgtatgagga cccagccggc
     1921 cccctcgaaa gatacctgga aggactcgcc gctgccgcgt cccgtaaagg tcttgaagga
     1981 gacatcgctc ctgacgcccg tcctaacgcc gccgctccag gcgatggcgg actcggggtc
     2041 ggcgaaggtc ggggtcgagg tgtcgaggag gacgggctca ccgtcggagg tgacggcgac
     2101 cttgcccgag ccttccagga cgacgttgaa gaggccgccc gcgaggcggc ccgcgccctc
     2161 gacgcgctct atgtcccact ctatgcctgg ctcgaaggcg aggatgttgt cgccgttgac
     2221 ggtcatacgc tcgttatgga ggtccaggat catgatcttg cgcttgtcca tcgccaggaa
     2281 gaggttgccc gtcccgttgg ccgccattag cttcacgccc tcgccggtga cggccttctt
     2341 gaagaaacgc cccaggccac cgctcttgtg ctcgaagctg acgtcgccct ggtaggcgac
     2401 catcgagccg agcttggcct ggatctggtc gttctccaac ttcaccttga gcatcgccga
     2461 gttctgcagc gtgaaccgac cttccggcgt aacctcccga aactgctcca ggttcgtctc
     2521 agtcaccgca ctaccttcct ttccgatcgc aatgaagata ctacccgggt acgcagcgcc
     2581 cgcctagaag tttcctagaa catcctgcca ccaggaggga cccggagatc cggggcgagg
     2641 agcactacct catcattctc gtcgggcacg cccaggacga gcacctcgct ctcgaagccc
     2701 gcgatcctct tcggcggaaa gttgatcacg gccaagacct gccgaccaac gagtacctca
     2761 ggctcatagt tagcagtgag ttgggcactg gtcttcctgg ttccgacctc cggcccgaag
     2821 tccacgacga gcctgatcga aggcttgcgt gcctccggga aagggtcggc cccaatgacc
     2881 gtgccgaccc ttacatccac cttctcgaaa tcatcccaac ctatctcagg caatacgtcc
     2941 tccctcctaa aggctacagg ttacggccat caggtagcac ctatttcttt ccagagccgg
     3001 cacagtctat acctgaaacc tgtagcctga cacctattac ggaagattcc tgacctcgaa
     3061 gagctctctc acgacgtgga ggttgagctt ccccggcagg aaggtatcac gcgaccactg
     3121 ggcggggtgc ccgtctgtca ggtacatggt ctggatcagc gagagcgccg cggaatcgac
     3181 tctcaggccg agtctttcgc cggtagggtc tttcggaccg atcatgacgg ccccgatgga
     3241 catctggctg tagcctacgg gcacgccttc tgagaccagg agatccaata gcatagcgtc
     3301 agggcgaaac cgctccttga cctcgtccac cccgattatg ctctggagca caacgtctac
     3361 catccatgcg gccgggtttc cttccaccaa caaagcacgc gacaccctgg ccagcggcgt
     3421 accttcagcg acctccagtg ccgcagcctc atccggcgag gcaccgacag gctcgatagt
     3481 aaggcccgag ctaccgagtt cgagtcccag cctcgcagcg tggaccgtgt aagtctccag
     3541 ccgctccagg cccgccgaga gtcgcgcccc ttccggcggt cgaaccagga acgtcccact
     3601 cccctggcgc cgctccagca acccccgctc tacgagtcgc gccagccctg cccgcaccgt
     3661 tacccgcgag acgcctagag cgtcgaccaa cccggtctcg ggcggtatcc gttcccccgc
     3721 cctgtagcga ccctgcaaca gaaggtcctc gatgcgctgc tccacctgga ggtagagggg
     3781 atccctcttc ctcggtcccg ggttcagtgg actatacctg atcttccacc ccttctctcg
     3841 atcgaaacgt ctgacatacc ccggaatgct acccgaggaa ccgccgtccg caagaattct
     3901 aggcgttcgt cctcttgtat gacaaattgg tattaactat gatgccatcg tggccagagg
     3961 gccggtacgg ggacggccat gacggggaat gggttcttcg ggaagggagt tcgggatgag
     4021 cgagcaggaa agacggacaa ggcagggggc gggctacgag aacgtcgggt ccgattacct
     4081 ggagcagcgc cagctgcaaa aaggggccgc gggctgggtg ctcttggccg gtctaggagt
     4141 ggcctacgtt atttcgggcg actttgcggg ctggcatttc gggctaggcg cgggcgggtg
     4201 gggcgggttg ctcatcgcca cggtgctcat ggccacgatg tacacggcga tggtctttgc
     4261 cctggccgag ttgtcgtcca cgattccgac ggcgggcggg ggctacgggt tcgcgcggcg
     4321 ggcgctgggg ccgtggggag ggtttatgac ggggacggcg atcctgatcg agtacgccat
     4381 cgctcctgcg gctatcgcga cgttcatcgg cggttacgtg gagaccctca ccgggatcgg
     4441 cggttggatc gtctacctcg tgtgctactt gatcttcgta ggcattcacc tctacggcgt
     4501 cggtgaggcg ctcaaggtga cgttcggcat aacagtcgta gccgtcgtgg cgctggcggc
     4561 gttcgtgatc ggcatgatcc cacagttcga ggtggcgaac ctgttcgaca tagatccgac
     4621 gggcgcggcc ggtgcgagct cgttcttgcc gttcggggtg gtgggcatct gggcggcgtt
     4681 cccgttcgcg atctggttct tcctcgcgat agagggcgtg ccgctcgctg ccgaggagac
     4741 cagggaccca gcgacagaca tgcccaaagg gctcatcgcc ggcatggcgg cgctgctcct
     4801 gttcgccgta ctcgtcctct tcttcggtcc tgggggtgcc ggttcggcgg cattcacgag
     4861 cggcgagata gtgctcgccc cgtccgtgct gcccctggcg atggaggagg cctacggcgg
     4921 tggcaacgcg ctcgtcacct tcgtcaatat cgtggggtta gcgggtctga tcgcgagctt
     4981 cttctcgctc atcttcgcct actcgcgcca gatgttcgcc ctgtccaggg ccggctacct
     5041 ccccaggtta ctctcggtca ccggtgggcg gaggacgccg tgggtggccc tgatagtccc
     5101 cggcgccatc ggcctggtcc ttgcgatcgc gggcggcagt gccggcggtg ccacgcttct
     5161 taacagcgcc gtcttcggcg ccacgatctc ttacgcgctg atgatgctct cgcacatagt
     5221 gctgcgccaa cgcgagcctg atctggatcg tccctatcgc acgccgggcg gcgtggccac
     5281 atcgggtgtg gcactcgtgc tggcggttct tgcggtcatc gcgaccttta tcgtagacgt
     5341 cagggcagcg ctcatcatgc tcggggtcta cgcactcttc ctcgcctact tcggcctcta
     5401 cagccggcac cacctcgtcg cccaggcgcc ggaggaagag ttcgccgcga tagagaaggc
     5461 cgagtccgaa ctggccggaa actagagagg agcaggaatt ggacacgacg caggcggctg
     5521 gacgcctcga cctcgacacc ctgcgctcgg aggtcgaggc cggaaacata gacaccgtcc
     5581 tcctcgcgat gacggacatg cagggccggc tccagggaaa gcgtctcacg gcgactcact
     5641 tcctcgacga ggtcgtcgag cacgacgcgg aaggctgcaa ctacctgctt gccgtggacg
     5701 tggagatgaa caccgtagag ggatacgcga tgtcctcctg ggagcgtggc tacggcgact
     5761 tcgtcttcaa gcctgacatg gacaccctga ggtacgtgcc ctggcaggag gggacggcgt
     5821 tcgtgacgtg cgacctcgtc tgggaggacg gctccccggt cgtggcctcg ccccggcagg
     5881 tcctcaagcg ccagctaggg aggctctccg aacgcggcct ggaagccttc gtggggaccg
     5941 aactcgagtt catagtcttc atggactcct acgaggaagc ctggcgtaag gggtatcgtg
     6001 atctcgaacc agcaaactac tacaacgtcg actactcgct gctcgggacg gcgcgcatcg
     6061 agcccttgct gcggcgcatc cgcaactcca tggcgggggc cgggctcgcg gtggaggatg
     6121 cgaagggcga gtgcaacttc gggcagcacg agatcaactt ccggttcgac cgcgctctag
     6181 agaccgcgga cgggcacgcg atctacaaga acggcgccaa ggagatcgcc gcccaggagg
     6241 gctgttccat ctccttcatg cccaagttcg acgaacgcga gggaaactcc tgccacatac
     6301 atttgagcct ccgcagagag gacggagcac cggtcttcac agacgggcac ggtttctccc
     6361 cgacgttcga gcacttcata gccgggcagc tcgcctgcct gcgcgagctc acgctgttct
     6421 tcgccccgaa cataaactcg tacaaacgct acgccaaggg ctccttcgcc ccgacggcgg
     6481 tggcgtgggg caaggacaac cgcacctgta gcttgcgcgt cgtcggccac ggcaactctt
     6541 tgcgcgtcga gaaccggctg cctggtgggg acgtcaaccc ttacctggcg atagcggcca
     6601 tgatcgccgc tggcctgcac ggcatcgaga acgagctctc cctggaagag gagttcatcg
     6661 gtaacgctta cgaggcggac aagccgcacg tgccgacgaa cctgcgcgag gcacgggagc
     6721 tgtttgcggc gagcgaggcg gccaggagtg ccttcgggga agaagtagta gagcactact
     6781 tgaacatggc gcgggtggag atcgaagctt tcgaagctgc cgttaccgac tgggaacgct
     6841 accggaactt cgagcgcctg tgaaggcggg atccaggccc acaatcggcg tgacggcggc
     6901 gaccgagagg gttagctacg gcgtctggaa cgagataccg gcggtcatca gcccggcgcg
     6961 ctacctccag gccgtccagc gcgccggcgg acgtcccgtc ctgctgcctc cagatccgga
     7021 ggacgcagag gaccctggcg gggtgctgga tcttctcgac gcgctcgtcg tcacgggagg
     7081 agctggggac ctggat
//
LOCUS       DATMVF010000038        10028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_189278, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000038 DATMVF010000000
VERSION     DATMVF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189278"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..187)
                     /locus_tag="VN178_01580"
     CDS             complement(38..187)
                     /locus_tag="VN178_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79684.1"
                     /translation="MHPSSLHRRVRTLRRLLEPLRRSILTELVGEPETLLVDLTLLPV
                     LHPVR"
     gene            complement(266..436)
                     /locus_tag="VN178_01585"
     CDS             complement(266..436)
                     /locus_tag="VN178_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79685.1"
                     /translation="MAHPQHTPPLVPLEEAITALFCLIEDAYASLNPNATSFETLKKL
                     SDSEVLTLALLQ"
     gene            complement(516..1466)
                     /locus_tag="VN178_01590"
     CDS             complement(516..1466)
                     /locus_tag="VN178_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWS79686.1"
                     /translation="MAIELGLIGVPTSMGAFAPGQEKGPRALREAGLLGRLSRAGIEV
                     VDHGNGVEVRRWRPDRANRYAQNLPAVVEVVREAAGRVGEAHAAGHLPLVVGGDCTVE
                     LGTVAGLLPSKGSGGRLGLIYFDVHPDLNVPASVGEGALDWMGMAHMLGEDEAAEPLA
                     RFGPRFPLLADEDVFFFSYGPEQATEWEREVIGRRGLRGIPVEEVAADPEGAAARALA
                     EMEPRFDRLLVHFDVDTVDFTDLPLSENTGRNEGLPFDAAMRSLGTLLGSDRLAAVTV
                     TEFNPDHGEEDGSTAEALAEGLASALSCSPALGNTTSTTS"
     gene            complement(1792..2925)
                     /locus_tag="VN178_01595"
     CDS             complement(1792..2925)
                     /locus_tag="VN178_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS79687.1"
                     /translation="MNTSTSAELVRQEIIRLCHAGLDSRTFRIESVKRLKKAIPVDAS
                     FFATADPATLRFTGAVLDDVLASATAQFLENEFMREDAVKFARLARGRSPVESLGAAT
                     RGELVLSPRYQEILAPMDLGDELRAALVVGSKCWGLMCLHRERSSPNFTPAEAAFLAK
                     LTPHLAEGLRTALLIGDARVTSLPPDGPGLLLLGDDLSLAAITPAAEGWLAEVAKSDW
                     PSSSELPEVVYAVAARLLALERGGDNAPPDLMPRIRLRSASGRWLVLHASRLRTADAE
                     GQIAVIFEEARPSEIAPLIVDAYGLTKREGEITRLVLRGLSTAEVSNELYITPNTVRD
                     HFKSIFDKVGVRSRRELVAGIFAQQYQPRMATGHGPDADGWFA"
     gene            3050..3481
                     /locus_tag="VN178_01600"
     CDS             3050..3481
                     /locus_tag="VN178_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWS79688.1"
                     /translation="MTAEENKELVHRFVEEFWNQGKTTAADELMAPDAEIHMPTGEVL
                     DPDELKNFVGTWRESFPDWYSTFEELIAEGDRVAERWTCRGTHLGELQGIPPTGKRVE
                     APGSVFYRIADGKIVEFRGQLDMMGLMQQLGVIPSPRQAEA"
     gene            complement(3519..4421)
                     /locus_tag="VN178_01605"
     CDS             complement(3519..4421)
                     /locus_tag="VN178_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HWS79689.1"
                     /translation="MESSGVAPRVVVVGDLLYDLLAKVEGEVTFGTDTFVPIRVAAGG
                     SGANAAAWFASSGIETHFVGRVGDDPFGGFLVQELERTGVSPHLARDPSLATGKVFVL
                     VDGAGERTMITDRGAGEALSPEDLPGSLFRRGHLHLSGYTLSGGSRRETAIKALRLAR
                     RSGMTFSVDPSSVPLLQAVGPGFFLESTRGADSCFPNLEEGALLAGTEEPERIAETLL
                     GYYFAVVLKLGPEGALYAGADGVRVRVPASPVRVVDTTGAGDALCAGFLSARLFGDSP
                     AEALWRGVGLASRAIERVGGRPKS"
     gene            complement(4425..5348)
                     /locus_tag="VN178_01610"
     CDS             complement(4425..5348)
                     /locus_tag="VN178_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HWS79690.1"
                     /translation="MSSEIRADVREALSEGTPVVALESTLISHGLPRPENLSVAREAE
                     RAVRAEGAVPATIGVIGGTAKVGLDEVELELLASAADVPKLSARDLPVAAAKASHGAT
                     TVAATAHLAALAGIRIFATGGLGGVHRGARESWDVSADLSALARTPVAVVCSGVKSIL
                     DVPATLEYLETVGVPVVGFRTLRFPGFYLTDSGSKLDWSVEDEREAARVIQAMAQSGL
                     GGSGLLIANPLPEEEQLDRDLHDRALISGLEGLEDRGVRGKDVTPFLLDRFATETRGE
                     SLRVNRLIIVNNAGLAARIARELSGLANGGS"
     gene            complement(5345..6007)
                     /locus_tag="VN178_01615"
     CDS             complement(5345..6007)
                     /locus_tag="VN178_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWS79691.1"
                     /translation="MCGRYTLSTPAGRLAEEFQLAEGAVDLTPSYNVAPTQEVAAVLE
                     EEGERRLEMFRWGLIPSWADDPGIGARMINARSETAAEKPSFRSAFRRRRCLIAADGF
                     YEWKRENGGKQPYYFHMQEGRPFAFAGLWESWDKGEVALRSCAILTTRANSVLEGIHD
                     RMPVILPSGAYDLWLDPDADREQLADLLIPYPGDDLETYPVSRYVNSPRNNDEHCIEP
                     AA"
     gene            complement(6102..6779)
                     /locus_tag="VN178_01620"
     CDS             complement(6102..6779)
                     /locus_tag="VN178_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HWS79692.1"
                     /translation="MRIFSFPPFEDRRDAGRQLAGKLSRFEDERPVVFALPRGGVPVG
                     YEISRSLKAPLDVFISRKLGAPGQPEFGIGAVAAGGVRILNQDVVRRLGISDDYVEQI
                     TAQELAEVRRRLRYFRGERPETEVVGRTAILVDDGLATGVTARAAVQALRLRDPGRLV
                     LAAPVCAAQTASHFKRAVEELVCLETPSDLGAIGFWYKNFEQTSDEEVVELLERARSE
                     QSAEGSP"
     gene            complement(6784..7803)
                     /locus_tag="VN178_01625"
     CDS             complement(6784..7803)
                     /locus_tag="VN178_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWS79693.1"
                     /translation="MKSLHVYCDPASKGHVNDLAGVESPERFPAALAGVERAEQSGVH
                     VEWRPCSPAQRETLVAVHDAAYLDGLREMSAAGEGYLSLDTSVGAGSWEAASFASGAA
                     SSAVESALAGETAFAVARPPGHHAGRDYGMGFCLTNHAAVAAGHALSRGLNRVAILDW
                     DVHHGNGTQDIFYGDGRVLYLSVHQSPFYPGTGEAREVGEGEGSGFTVNVPLPAGSKE
                     DIYAAAFAGVFLPVLREFGPEVILISAGFDAHADDLLGGMALSSDSFGRFAALISDLS
                     REVGAAPPAFVLEGGYNLDALTESVAKTMQGVGERVPEWGYAGGMRHIQESREALAPF
                     WRSLR"
     gene            7913..9289
                     /locus_tag="VN178_01630"
     CDS             7913..9289
                     /locus_tag="VN178_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWS79694.1"
                     /translation="MEEGAARGRSVSRLNVLIAITAAIIGLIYGYDLGSIASALLFLV
                     PAFELSTFMTSVVTSAVVLGQLFGAFFAGRISNTIGRKRSLVLVALGYAAFAGLQGLA
                     PNEWFLTVVRFLLGLAIGISIVVAPAYIAESAPVSVRGSMLVTFQIATTSGIAVAYFV
                     GAALAPTESWRLILSLSAIPAVIVLILIVRLPDTARWLLMNGRREEATDLLRRVEPDM
                     DPDEEVRRIEHDLSYEEKGSFAELFQGRFRRAGIFVVGLGFLVQITGINAIVYYSPTI
                     IQEVGVTSPTGAIVATGFVQIAGVIAEVVAFLVVDRWGRRPTLLTGIGMMGVANAILV
                     LAYALDSPATFTLLGILLFTVGFNFGYGSLVWVYASESFPVRLRTQGGSAMLTADLFA
                     NFIVGVVFLNALGALGGSVTFGIFLALALVSFAFVYVLAPETKGRPLEAIRTYWYNGG
                     RWPEETST"
     gene            9706..>10028
                     /locus_tag="VN178_01635"
     CDS             9706..>10028
                     /locus_tag="VN178_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWS79695.1"
                     /translation="MRQLAAALLTCALFSVVLLTGSPSEANAQGYYGASGQAVVDNAE
                     SYLGMPYGASGMTCSGFTSMVFGDLGVYLPASPGAQYAYGTPSYGKAGDLVFFDETGY
                     GISHVG"
BASE COUNT         1698 a         3393 c         3138 g         1799 t
ORIGIN      
        1 acccatgccg cctcctcgaa gccctcagcc gactgcctca cctgacgggg tgcaagaccg
       61 ggaggagggt caagtcgacg aggagcgtct ccggctctcc caccagttcg gtcaggatcg
      121 agcggcgcag gggttccaag aggcgcctga gcgtcctcac gcggcggtgc aaggaggatg
      181 ggtgcatccc cgcaactccc gggaggtgga cgaagaacct ccgggcgtcg cgcaggaagg
      241 agtgctcgct ctcgatgccc ctaagctact gcaagagcgc cagcgtcaag acctccgaat
      301 ccgaaagctt cttgagcgtc tcgaagctcg tggcgttggg gttgagggag gcgtaggcat
      361 cctcgatgag gcagaacagc gcggttatgg cctcttccag gggaacgagc ggcggggtat
      421 gctgtggatg agccatccgg ggctcctttg gtcgggtagt ggccggatgg ccttcttttt
      481 actcctaatc cacgcaacac tcatctagag gtctctcacg aagtcgtcga ggttgtgttc
      541 ccgagcgcgg gcgagcacga cagagcgctg gcgagcccct cggccaacgc ctctgccgtg
      601 gacccatcct cctcgccgtg gtcggggttg aactccgtga cggtcaccgc tgcgagccga
      661 tcgctcccca gcagcgtacc cagcgaccgc atcgctgcgt cgaatggcaa accctcgttg
      721 cgcccggtgt tctccgaaag cggtaggtcg gtgaagtcca ccgtgtccac gtcgaaatgc
      781 acgagcagac ggtcgaagcg aggctccatt tccgcaaggg cgcgcgccgc ggcgccctct
      841 ggatcggcgg ccacctcctc gaccgggatt ccccggagcc cacggcgtcc gattacctcg
      901 cgctcccatt cggtcgcctg ttcgggaccg taggagaaga agaagacatc ctcgtctgcc
      961 agcaagggga agcgcggtcc gaaacgagca agcggctcgg ccgcctcatc ctcaccgagc
     1021 atgtgcgcca tgcccatcca atcgagtgcc ccctcgccca cactggcggg cacgttgagg
     1081 tcgggatgga cgtcgaagta gatcagtccc agtcgtcccc ctgacccttt ggatggcaga
     1141 agtcccgcga ccgtgcccag ctcgacggtg cagtccccgc caacaaccag aggcaggtgc
     1201 cccgcggcgt gcgcctcccc gaccctgccg gccgcttcgc gtacgacttc cacgacggcg
     1261 ggaagattct gcgcgtatcg gttggccctg tccggccgcc agcgccgcac ctccacacca
     1321 ttcccgtggt ccaccacctc gatacccgcg cgggagagcc gcccgaggag gccggcctcc
     1381 ctgagtgccc gtggaccctt ctcttggcca ggggcgaagg cccccatact ggtcgggacc
     1441 ccgatcaagc ccagctcaat agccacgcgt cctccttttc cgaatcgtgt tcactgcccg
     1501 gtcatgtcgc agaacgtctg tataccctat tgcgacctac tcatctgtcc tatagccgct
     1561 gcaagaatac ttcgccaccg ctcccggatc taattgcacc ctcaagccgg ggtgacgagg
     1621 atagacaaca tgtcgtcggc gtccaacttc caacccccga ctgtaatgct tgaaatcatc
     1681 cagcctctgc atacggcacg tattagtacc cgtaaagggt tccggccgaa gggggtgccg
     1741 gtatgaagac taaacctcca tctgcgtcgg tgtaaagcca acctgcggct ctcaggcaaa
     1801 ccacccatcg gcgtctggtc cgtggccggt ggccatgcgc ggctggtact gttgggcaaa
     1861 gatcccggcg acgagttctc tacggctgcg aacgccgacc ttgtcgaaga tggacttgaa
     1921 gtgatcgcgc accgtgtttg gcgtgatgta gagttcgttg gagacttcgg ccgtggaaag
     1981 gccgcgcagg acgagcctgg tgatctcgcc ctcacgcttg gtcaggccgt acgcatcgac
     2041 gatcagaggc gcgatctccg agggacgcgc ctcctcgaag atgaccgcta tctgcccctc
     2101 ggcatctgct gtcctgagcc tcgaggcgtg cagtaccagc caccgtccgg aggcgctcct
     2161 gaggcgtatc ctcggcatca ggtctggtgg tgcattgtcg ccgccacgct ctagcgcaag
     2221 caaccgagcg gcaacagcgt agaccacttc cggcagctcg ctggagctag gccaatcgct
     2281 ctttgccacc tccgccagcc acccctcggc cgctggtgta atagcagcca gtgagagatc
     2341 atctccaagt aatagcaacc ccggcccgtc cggcggcagg gaggtcactc gcgcatcgcc
     2401 gatcagcagg gcggtgcgca gaccctcggc caaatgtggg gtgagtttcg ccaggaaggc
     2461 ggcctcggcg ggcgtgaagt tggggctgga gcgctcgcgg tgcagacaca tgaggcccca
     2521 gcacttggag cccaccacca gggcagctct caactcgtcc cccaggtcca tcggggcgag
     2581 gatctcctgg tagcgcgggc tgaggaccaa ctcacctcgg gtcgcagccc ccaagctctc
     2641 gaccggcgag cgtcccctcg ccagacgcgc aaacttgacg gcgtcctctc gcatgaactc
     2701 gttctcgagg aactgggccg tggcgctcgc caggacgtcg tcgagtacgg ctcctgtaaa
     2761 gcgcagggtc gccgggtcgg cggtagcgaa gaacgaagcg tctaccggaa tggccttctt
     2821 cagacgcttt acactctcta tcctgaacgt ccttgagtca agcccggcat gacagagacg
     2881 gatgatctct tgcctgacaa gctccgccga ggtgcttgtg ttcatggtgt ggccatttta
     2941 caagtaaccg gagtaccaaa atatcccaca tttgtgggat tacttttgtc tgccgcccgt
     3001 actaccgtcc atccaatgag actcaggcga acgtgaagga ggtctcgaca tgacggctga
     3061 agagaacaag gagctggtgc accggttcgt ggaggagttc tggaaccagg gaaagacgac
     3121 cgccgcggac gagctgatgg cgccagacgc cgagatccac atgcccaccg gagaggtgct
     3181 cgatcccgat gagctcaaga acttcgtcgg tacgtggcgc gaatcatttc cagactggta
     3241 ttccaccttc gaggagctga tcgctgaagg ggatagggtg gccgagaggt ggacctgtcg
     3301 gggcacgcac ctgggcgagc tgcagggcat ccctcccacc ggcaagcgcg tagaggcgcc
     3361 aggcagcgtc ttctatcgaa tagccgacgg gaagatcgtg gagttccgag gacagctcga
     3421 tatgatgggc ctgatgcagc agttgggcgt gataccgtca ccgcggcaag ccgaggcttg
     3481 atgtgccctg caacccgtgc ctggccggcg gcaggggatc agcttttcgg gcgaccaccc
     3541 acgcgctcga tggcgcgcga cgcgagcccg acgccccgcc acagcgcctc ggcgggcgag
     3601 tccccaaata gcctggcgga caggaagccc gcacagaggg cgtcccccgc ccccgtggtg
     3661 tcaaccaccc gcactggcga tgcgggcacg cgtaccctta ctccgtccgc gccggcgtat
     3721 agggcgccct cggggccgag cttgaggacg acggcaaagt agtagccaag cagcgtctcc
     3781 gcgatgcgct cgggttcttc ggtcccggcc agcaacgcac cctcttccag attcgggaag
     3841 cacgaatccg cgccgcgggt cgactccagg aagaatccgg gaccaacggc ctgaagcagt
     3901 gggacggatg aagggtccac ggagaacgtc atcccgctcc ggcgcgccag gcgcaaggct
     3961 ttgatcgcgg tctccctgcg gctgccgccg gagagggtgt aaccggacag gtgcaggtgt
     4021 cctctacgga agagtgatcc tgggaggtct tcggggctga gggcctcccc ggcgcctcgg
     4081 tcggtgatca tggtcctctc cccggcgccg tccacgagta cgaagacctt gcccgtagcc
     4141 agggacgggt cccgcgccag gtgcgggctc acgccggtcc gctccagctc ctgtaccagg
     4201 aaccccccga aggggtcgtc cccgaccctg ccgacgaaat gagtctcgat gccggaggag
     4261 gcgaaccagg ccgccgcgtt ggcgcccgag ccgccggctg cgacccggat cgggacgaag
     4321 gtgtccgtcc cgaacgtcac ctctccctct accttcgcga gcaggtcgta gaggaggtcc
     4381 cccaccacga cgacccgagg ggcgaccccg gagctctcca attctcaaga ccccccgttg
     4441 gcgagtcccg atagctccct ggcgatgcgc gccgcgagcc cggcgttgtt cacgatgatg
     4501 agcctgttca ccctcaagct ctcccctctc gtctccgtcg cgaaacggtc caggaggaag
     4561 ggcgtcacgt ccttgccgcg cacgcccctg tcttcgaggc cttcgaggcc ggagatcaag
     4621 gcccggtcgt ggaggtcgcg gtcgagctgt tcttcttcgg gcagggggtt cgcgatcaag
     4681 agcccggagc ctccgaggcc cgattgcgcc atagcctgga tcacacgcgc tgcctccctc
     4741 tcgtcctcca ccgaccagtc cagcttgctc cccgagtcgg taaggtagaa gccagggaat
     4801 cgtaaggtgc ggaagcctac gacgggcacg cccacggtct ccaggtactc cagcgtcgcc
     4861 ggaacgtcga ggatggactt gaccccggag cagacgacgg ccacgggcgt tcgggcgagg
     4921 gcagagaggt ctgcggatac gtcccaactc tcgcgcgccc cccggtgcac gccgccgagg
     4981 ccgcccgtgg caaatattct gatccctgct agcgccgcga ggtgcgcagt ggcggcgacg
     5041 gtggtggccc cgtggctcgc cttcgcggcg gccacgggca ggtcacgcgc cgagagctta
     5101 gggacgtcag ccgcgctcgc caggagctcg agctccactt cgtccagccc gaccttggcc
     5161 gtaccgccga tgacgcctat cgtcgctggc acggcgcctt cggcgcgcac ggcacgctct
     5221 gcttcgcggg cgacactcag gttttcgggg cggggcaatc cgtgggagat cagggtcgac
     5281 tcgagcgcca cgacgggcgt tccttcactc agggcctccc taacgtcggc cctgatctcg
     5341 ctgctcaagc ggccggctct atgcagtgct catcgttgtt gcgcgggctg ttgacgtagc
     5401 ggctcaccgg gtaagtctcg aggtcgtcgc cgggataggg gatcagcaag tcggcgagct
     5461 gctccctgtc ggcgtcggga tctagccaga ggtcgtaagc gccggaaggc agtatcacgg
     5521 gcatcctgtc gtggatccct tcgagcacgg agttcgccct ggtggtcaga atagcgcacg
     5581 agcggagagc cacttcaccc ttgtcccagc tctcccagag tccggcgaag gcgaagggcc
     5641 ggccttcttg catgtggaag tagtagggct gcttgccccc gttctcgcgc ttccactcgt
     5701 agaacccgtc ggccgcgatc aggcaccgcc gcctgcggaa ggcactgcgg aaagagggtt
     5761 tctccgcggc cgtctcgctg cgggcgttga tcatgcgcgc cccgatcccg gggtcgtccg
     5821 cccacgaagg aatgagcccc cacctgaaca tctccagacg tcgctcccct tcctcctcca
     5881 gaaccgcagc aacctcctga gtcggggcga cgttgtagct cggcgtaagg tcgaccgcac
     5941 cttcagccaa ctggaactcc tcggccagcc ggccggccgg ggtagaaagc gtatacctgc
     6001 cgcacatatc tctcacctct ccaaaagtcc acgaacgtac tgtaattgta ccggcccgag
     6061 gcccgtctcc caccggccgg ttgaggcggc tcggggtgct tctacgggct cccttctgcg
     6121 ctctgttcgc tcctggctct ctcgagcagt tcgacgacct cctcgtccga ggtctgctcg
     6181 aagttcttgt accagaagcc tatggccccg aggtccgagg gggtctccag gcagaccagc
     6241 tcttccaccg ctctcttgaa gtgagaggcg gtctgggcgg cgcataccgg cgctgccagg
     6301 accaggcgtc ccggatcgcg caaccgcagg gcttgtacgg cggcgcgggc cgtcaccccg
     6361 gtagcgagtc cgtcgtcgac gaggatcgcg gtccgcccca ccacttccgt ctctggcctc
     6421 tcccccctga agtagcgtag cctccgccgc acctccgcga gctcctgagc cgtgatctgc
     6481 tcgacgtagt cgtcggagat gccgagacgc ctgaccacgt cctggttgag gatgcgcacg
     6541 ccgccggccg caacggcccc gatgccgaac tcaggctgcc ctggcgcccc gagcttgcgc
     6601 gagatgaaca cgtcgagcgg tgctttgaga gagcgcgaga tctcgtaccc cacaggcacc
     6661 cctcctcttg gtagggcgaa gaccacgggc cgctcgtcct cgaagcggga cagcttcccg
     6721 gcaagctggc ggcccgcgtc cctgcggtcc tcgaacggag gaaagctgaa gatcctcata
     6781 atctcagcgg aggctccgcc agaacggcgc gagggcctcg cggctctcct ggatgtgcct
     6841 cataccaccc gcataccccc attccgggac cctctcccca acaccctgca tggtcttggc
     6901 gacgctctcg gtcagggcgt cgaggttgta gcctccctcg aggacgaacg cagggggtgc
     6961 ggcccctacc tcgcgggaga ggtctgagat cagggcggcg aagcgtccga aagagtcgct
     7021 actcaaagcc atgcccccca gtagatcgtc tgcgtgggcg tcgaaaccgg cggagatcag
     7081 gatcacctcg ggcccaaact cccgcagaac gggcaggaaa accccggcga aagcggccgc
     7141 atagatgtct tccttcgagc cggcgggcag cggcacgttc accgtgaagc cagacccttc
     7201 tccttctccc acctccctcg cctcgcccgt tccggggtag aagggactct gatgcaccga
     7261 gaggtagagg acgcgtccgt cgccgtagaa gatgtcctgt gtgccgttgc cgtggtgcac
     7321 gtcccagtcc aggatggcga cccggttgag acctctggaa agggcgtgcc cggccgcgac
     7381 ggccgcgtgg ttcgtgaggc agaaccccat cccgtagtcc ctccccgcgt ggtggccagg
     7441 aggacgggcg acggcgaagg ccgtctcgcc ggcgagggcg ctctcgaccg ccgagctggc
     7501 ggccccgctc gcgaaagagg ccgcctccca ggaacccgcg ccgacggacg tatccaggct
     7561 gaggtagccc tctcccgccg cggacatctc tcgcaggccg tccagatacg ccgcatcgtg
     7621 gacggcgacg agggtttccc tctgggcggg actacacggg cgccactcca cgtgcacgcc
     7681 gctttgctcg gccctttcga cccctgcgag ggccgcgggg aacctctctg gggattccac
     7741 accggcgagg tcgtttacgt ggcccttcga ggccgggtca cagtagacgt gcaaactctt
     7801 caccctccca ggtcaagata ccatctccgc gttttgtcgg gttctagatc ctagcccact
     7861 atgctactat gccggcatct cggatgggac ccctggggaa agaaggtttg ccgtggaaga
     7921 aggggcagcg cgcgggcgga gcgtatcgag gctaaacgtc ctcatcgcca tcacggccgc
     7981 gatcatcggc ctgatctacg gctacgacct gggctctatc gcgagcgcac tcctcttcct
     8041 cgtcccggcc ttcgaactct ccactttcat gacctccgtt gttacctcag ccgttgtgct
     8101 cggacagttg ttcggggcgt tctttgccgg gcgtatctcc aacaccatcg ggcgcaaacg
     8161 gtctctggtg ctggtcgcgc tcggctacgc ggcgttcgcc ggtctgcagg ggctggcccc
     8221 caacgaatgg ttcctgaccg tcgtcaggtt cctcctcggt ctcgccatcg gcatctccat
     8281 agtcgtggcg ccggcataca tagccgagtc cgcgcccgtc agcgtcaggg gctcgatgct
     8341 cgtaaccttc cagatagcga ctacctcggg tatcgcggtc gcctacttcg tcggcgcggc
     8401 cctcgcgccc accgagagct ggcgtctgat actctccctc tccgccatcc cggccgtcat
     8461 cgtgttgatc ctcatcgtcc gcctgcctga cacggcccgc tggctcctga tgaacggccg
     8521 acgcgaggag gcgaccgatc tcctgcgcag ggtcgaaccc gacatggacc ccgacgagga
     8581 agtcaggagg atcgagcacg accttagcta cgaggagaag gggagcttcg ccgagctctt
     8641 ccaggggcgt ttccgtagag ctggcatctt cgtcgtgggc ctcggcttct tggtccagat
     8701 cacgggcatc aacgccatcg tctactacag cccgacgatc atccaggagg tcggcgtcac
     8761 gagtcctacg ggcgcgatcg tagcgaccgg tttcgtccag atcgcgggcg tgatcgccga
     8821 ggtcgtcgcc ttcctggtcg tggacaggtg ggggagacgg cccaccctgc tgaccggtat
     8881 cggcatgatg ggggtcgcca acgctatcct cgtcctggcc tacgcgctgg actcccctgc
     8941 gacgttcacc ctgctcggca tcctgctctt caccgtgggc ttcaacttcg gctacggttc
     9001 gctggtctgg gtctatgcct ccgagagctt ccccgtgcgc ctgcgcaccc agggcggctc
     9061 ggccatgctg acagcagacc tgttcgccaa cttcatcgtc ggagtcgtgt tcctgaacgc
     9121 cctcggcgcc ctcggaggct cggttacttt cggtatcttc ctggccctgg ccctggtctc
     9181 gttcgccttc gtctacgtac tcgcccccga gaccaaggga aggccgctgg aggcgatacg
     9241 cacctactgg tacaacggtg ggcgctggcc cgaggagacc agcacctgag ccttccataa
     9301 ggtcgggatc agaccctcca tctcgccccc atggcaggaa cgaaggcggc gggcgagacg
     9361 cggcccctgt aggacctctg aatgatacac gtagcctgaa gattcgtact agtaccaggt
     9421 atggttttca gacgagtcag gaattgcttc tctattttcg aaagctgggt aatcccgagg
     9481 tctgcatcgg gcgtgcggcc tcgcgggcgc acgcgcttct ttgtgacccg cgcgtgcacg
     9541 cgcgggcgag atctacgacc ttcttcgggt tagcaccttc gctataccgg gagccctcgc
     9601 gccgcgatcg gcccattctt gcgcgggatt cgccttgaaa cggcgcgtag ctctgctact
     9661 atccaccgcc gttgtgcaag caagacaatt ggagggggat ctatcgtgcg gcaactcgct
     9721 gctgccctgc ttacttgtgc cctgttttcg gtcgtcctac taacgggctc accgtctgaa
     9781 gccaacgccc aaggctacta cggcgcctcg ggacaggcgg tcgtggacaa cgccgagtcc
     9841 tatctcggta tgccctatgg tgcctcgggc atgacctgtt cgggcttcac ctcgatggtc
     9901 ttcggcgacc tcggcgtgta cctgcccgcc tcccctggcg cccagtacgc ttacgggacg
     9961 ccttcctacg ggaaagccgg cgatctggtg ttcttcgacg agacaggcta cggtatctcg
    10021 cacgtcgg
//
LOCUS       DATMVF010000039         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_49291, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000039 DATMVF010000000
VERSION     DATMVF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49291"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VN178_01640"
     CDS             <1..701
                     /locus_tag="VN178_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWS79696.1"
                     /translation="EADRDVFVAGNLSCAWVYRPEDPKTHRRTRELFDRQLDVMTAEG
                     VDFVIAETFNWLGEAMIAAEAIKATGLPAMITMGPDNDPNSFEGYSPGEQVRRLADAG
                     VDIVGANCLRSPRYMLPIMEEMRAAVPDSVHMAAQPVAFHGTDERPNFVSLPEFPYEL
                     EKLTLPRAAMGEFARRARDLGYNYIGSCCGSVAAHVREMARALGKTSSDEREWRTRTG
                     IAQSGYELHRMTEQ"
     gene            765..1952
                     /gene="solA"
                     /locus_tag="VN178_01645"
     CDS             765..1952
                     /gene="solA"
                     /locus_tag="VN178_01645"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWS79697.1"
                     /translation="MNGTGKKAFEYIVVGCGGIGSAAVYWLSREAGPEVLGLEQFHLG
                     HDRGGSEDHSRIIRLSYHDQAYTTLTPHTYEAWSEVEDESGIQLVFETGGLDLESVED
                     EPAYINHYAGAMAAAGISYEELTAPEVMDRFPQFTLDSSVLGVYQEKAGFVDAGKANA
                     VHVALAKARGATVLDGAPVYGVRKVGNGVEVQTSAGAFSARRLVVAAGAWTNKVLGHL
                     GYTIPLTCTQEQVTYFQTPHLREFAPERFPVWIWHGGPDYTFYGLPVYGAVGSKAGQD
                     TGGDVVTVDTRTFDPNPRVLATLRDFLQRYIPRSLGPELYTKTCLYDMPPDREFVLGA
                     VPGIPQVTFFCGAGHAFKFAGLVGRILSELALEGRTRYQIGAFRPDREALTDPDYRPM
                     LVM"
     gene            1960..2529
                     /locus_tag="VN178_01650"
     CDS             1960..2529
                     /locus_tag="VN178_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HWS79698.1"
                     /translation="MESAEFYVENLDPASVVAVARQGARVALGEEARERVRTSRGRVE
                     IMIEGSRPVYGVTTGFGALANTRISAEKRLELQQSLLRSHAAGMGPFVEPEVVRAMTA
                     IRAKTLSAGYSGVRPELIDALIAFLNAGLSPAVPEYGSMGASGDLAPLAHVGLCLIGE
                     GRLLDRGEPVETSEALERAGIRPLRLATK"
BASE COUNT          470 a          856 c          830 g          446 t
ORIGIN      
        1 gtgaggccga tagagacgtg ttcgtcgccg gcaacctgag ctgcgcttgg gtctacaggc
       61 ctgaagaccc gaagacccac cgccgcaccc gcgagctctt cgaccgccag ctcgacgtaa
      121 tgaccgcgga gggtgttgac ttcgtcatcg cggagacctt caactggctc ggcgaagcca
      181 tgatcgccgc cgaagccatc aaggccacag gactgcccgc gatgataacc atgggtcccg
      241 acaacgatcc caactccttc gagggctatt cgccgggcga gcaggtaagg cgtctggccg
      301 acgcaggcgt ggacatcgta ggcgccaact gcctgcgctc tccccggtac atgctcccca
      361 tcatggagga gatgcgcgcc gccgtccccg attcggtcca catggcggcc caacccgtag
      421 ctttccacgg gacagacgag cggccgaact tcgtctcgct ccccgagttc ccttacgagt
      481 tggagaaatt gacgctcccc cgcgccgcta tgggagagtt cgcaaggagg gctcgcgacc
      541 tcggctacaa ctatatcggc tcttgctgcg gctcggtcgc ggcccacgtc agggagatgg
      601 cgagggcgct cggcaagacg tcctcagacg agcgcgagtg gcgtacgagg acgggcatag
      661 cacagtccgg ctatgagctc catcgtatga cggagcagta agccgcgaag agcggcgtcg
      721 aacccacagg aaggtccgta aatgacctcg ggccggaggc gggcatgaac ggaacaggaa
      781 agaaggcgtt cgagtacatc gtcgtgggtt gtggcggcat cgggagcgcc gccgtatact
      841 ggctctcccg cgaggccggc cctgaggtac tcggcctcga acaattccac ctcggccacg
      901 ataggggcgg ctcggaagac cactcgcgca tcatacgcct ctcctaccac gatcaggcct
      961 acaccaccct gacgccccac acctacgagg cctggtccga ggtcgaggac gagtccggta
     1021 ttcagctggt cttcgagacg ggcggcctcg acctggaatc cgtggaggac gagcccgctt
     1081 acatcaacca ctatgccggc gcgatggcgg ccgcaggcat ctcttatgag gaactcaccg
     1141 cgcccgaggt gatggaccgt ttcccgcagt tcacgctcga ttcgagcgtg ctaggcgtct
     1201 accaagagaa ggccggtttc gtggacgcgg gcaaggccaa cgccgtacac gttgcgctgg
     1261 caaaggccag aggcgcgacg gtcctcgacg gggcgccggt gtacggtgtg cgtaaggttg
     1321 gtaacggtgt cgaggtccag acgtcggcgg gagccttctc cgccagaagg ctcgtcgtcg
     1381 cggcaggcgc gtggaccaac aaggtactcg gtcatcttgg gtatacgatt cccctcacgt
     1441 gcacccagga gcaggtgacg tactttcaga cgccccattt gcgggagttc gcgcccgaga
     1501 ggtttccggt ctggatctgg cacggcggtc ccgactacac gttctacggt ctccccgtct
     1561 acggcgcggt aggctccaaa gccggccagg ataccggcgg tgacgtcgtg accgtggaca
     1621 cccgaacgtt cgacccgaat ccacgcgttc tcgccacctt gcgcgacttc ttgcagcgtt
     1681 acatcccacg ctcgctcggg cccgagctct acaccaagac ctgtctctac gacatgccgc
     1741 cagatcgcga gttcgtgctg ggcgccgtgc cagggatacc ccaggtcacc ttcttctgcg
     1801 gcgccggaca cgcgttcaag ttcgccggcc tcgtcggcag gatcttgagc gagcttgcgc
     1861 ttgagggccg cacgcgctac cagatagggg ccttccgacc cgatcgtgag gctctcacgg
     1921 acccggacta caggccgatg cttgttatgt agcccgagga tggagtccgc ggagttctac
     1981 gtagagaacc tcgacccggc ttctgttgtc gccgtggcca ggcagggggc gcgggtggcg
     2041 ctgggcgagg aggcgcgcga gcgcgttagg acttcccgcg ggcgcgtgga gatcatgatc
     2101 gaaggctccc gtccggtcta cggggtgacc acgggcttcg gggcgctagc gaacaccagg
     2161 atctctgcag agaagcgcct cgagctccag caatcacttc tccgcagcca cgccgccggt
     2221 atgggacctt tcgtcgagcc cgaggtcgtg cgggccatga cggcgatcag ggcgaagacc
     2281 ctatccgcgg gctactctgg agtccgtccc gaactcatcg acgccctgat agcgttccta
     2341 aacgcgggcc tcagccccgc cgtccccgag tacggctcta tgggcgcgag cggagatctc
     2401 gcacccctcg cccacgtcgg cctctgcctc atcggggaag gccggctgct agatcgggga
     2461 gaaccggtcg agaccagcga agccctggag cgcgccggta ttcggcccct ccggctcgcc
     2521 acaaagtaat ttctctccct gatcaacggc acggacggta tgctcgctat gctgctgctc
     2581 tccctcgaag acctcaaggt cc
//
LOCUS       DATMVF010000040         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_189566, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000040 DATMVF010000000
VERSION     DATMVF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189566"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VN178_01655"
     CDS             complement(<1..285)
                     /locus_tag="VN178_01655"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWS79699.1"
                     /translation="MSSAVRAPADSAMVVGLGSAVRAELLADLGVRVLVEEAELTPKP
                     ALVDRRGGGAHQDMNLEMFRRSAHALRPTFEDLAARAYLCAPSRRLREDLA"
     gene            complement(282..1928)
                     /gene="mdcA"
                     /locus_tag="VN178_01660"
     CDS             complement(282..1928)
                     /gene="mdcA"
                     /locus_tag="VN178_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HWS79700.1"
                     /translation="MLDPTSTGWHTRREDKARRLDRVAKVARGRVLPTEEIIPALEAL
                     IAPGDRVALEGDNQKQADFLSRSLAEVDPEKVHDLHMLISSISRPDHLDLFERDIATR
                     VDFSYAGPQSLRVAQLLDDGKLRIGAIHTYLELYARLFVDLVPTVALVAAESADAEGN
                     LYTGSNTEDTPTIVEATAFRDGIVIAQVNEVVDELPRVDVPGSWVDFAVVADRPYEVE
                     PLFTRDPRQISDVQVLIGMLAIRGVYERHRVTSLNHGIGFGTAAIELLLPTYAESLGL
                     RGKICRNWVLNPHPTLIPAIESGWVDTVTAFGGEVGMEDYVAARPDVFMTGRDGSARS
                     NRMAAQTAGLYAADLFIGSSLQMDSDANSSTVTEGRLAGFGGAPNLGSDARGRRHPSQ
                     AWLEMSTAEGEISRGRKLVVQMVETFGPDGAPRFVESLDAVRVGADAGLPVAPVMVYG
                     EDVTHVVTEEGVAYLYRARSPEERREALAAVAGVSPIGRGHDRRKEERLRKEGLVAFP
                     EDLGVRRADAKRSLLAAHSIGDLVEWSGDLYKPPDRFWSP"
     gene            complement(2109..>2501)
                     /locus_tag="VN178_01665"
     CDS             complement(2109..>2501)
                     /locus_tag="VN178_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWS79701.1"
                     /translation="ALERICESPEIVGACLAALEPIIREMETVQDDPIRYRELNFLFH
                     RSIISVSRSGLVRRLYAQIEGPLKIFLRRMFLAEGTVPRSFGEHVQILAAIEEADAKK
                     AQQRLEKHDIDGMRRAIASSLPGQLATD"
BASE COUNT          431 a          812 c          781 g          477 t
ORIGIN      
        1 ggccaggtcc tcacgtagtc gtctgctcgg cgcgcaaaga taggctcggg cggctaggtc
       61 ctcaaaggta gggcgcagcg cgtgggcgga gcgccggaac atctccaggt tcatgtcttg
      121 gtgcgcgccg cctcctcggc ggtcgaccag agccggcttt ggtgtgagct ccgcctcttc
      181 gaccaggacc cgaaccccca ggtcggccaa cagttcggcc cggacggccg accccaatcc
      241 gaccaccatt gcgctgtcag cgggcgcccg cacggcggaa ctcatgggct ccagaaccgg
      301 tccgggggct tgtacaaatc cccggaccat tcgacgagat ccccgatgga gtgcgccgcg
      361 agtagcgagc gtttggcgtc tgcccgccgt acaccgaggt cctcggggaa agccacgagc
      421 ccttccttgc gcagcctctc ttccttgcgc cggtcatgac ccctgccaat cgggctgacc
      481 ccggcgaccg cggcgagggc ctcccgtcgc tcctccgggc tgcgtgcgcg gtacaggtag
      541 gcgacgcctt cctcggtcac cacgtgtgtc acgtcctcgc cgtagaccat cacgggggcg
      601 acaggcaacc cggcgtcggc gcccacccgc accgcgtcca acgactctac gaaccggggt
      661 gccccatcag gcccgaacgt ctccaccatc tgcacaacga gcttgcgtcc gcgggagatc
      721 tccccctccg cggtgctcat ctccagccat gcctgggatg ggtggcgcct gccccgcgcg
      781 tcactgccca ggttgggcgc cccgccgaag cctgctagtc gtccctccgt gaccgtggaa
      841 ctgttagcgt ccgagtccat ctgcaaactg gagccgatga acagatctgc tgcgtagagt
      901 ccggcagttt gcgccgccat tcggttggag cgtgccgacc cgtcgcggcc ggtcatgaag
      961 acgtccggcc gtgcggcgac gtagtcctcc atgcctacct cgcctccgaa ggcggtgacg
     1021 gtatcaaccc acccgctttc gatggcgggg atcagagtgg ggtgcgggtt gagtacccag
     1081 ttgcggcaga tcttaccgcg caaccccaga gactcggcgt aggtcggcag tagcaactcg
     1141 atcgcggctg tgccgaaacc gataccgtgg ttgagcgagg tcaccctatg gcgctcgtaa
     1201 actccccgaa tagcaagcat ccctataagg acctgcacgt cagatatctg acgtggatca
     1261 cgggtaaaga gtggctcgac ctcatacggc cggtcagcca ccactgcgaa gtccacccac
     1321 gatcccggta cgtccacgcg aggtagttca tcgaccacct cattgacctg cgcgatcact
     1381 atcccatcgc ggaaagcggt ggcctcgacg atcgtcggag tgtcttcggt gttcgaacct
     1441 gtatagaggt tgccctcggc gtcggccgac tcggcggcca ccagcgcgac ggtcggcacg
     1501 aggtcgacga agaggcgggc atagagctcg aggtaagtgt gtatcgcgcc gatccgcagc
     1561 ttaccgtcgt ccaggagctg cgccacccgc aggctctggg gcccggcgta agaaaagtcg
     1621 acccgcgtgg cgatgtcacg ttcgaacaga tccaggtggt ccgggcggct gatgctggag
     1681 atgagcatgt gcaggtcgtg gaccttctcc ggatccacct cggccaggct gcgcgacagg
     1741 aagtcagcct gcttctggtt gtcgccctcc aatgcaaccc ggtcgccggg agcgatgagc
     1801 gcctccaaag ccgggatgat ctcctcggtg ggcaggacac gcccacgcgc gaccttcgcg
     1861 acccggtcca ggcggcgagc cttgtcttcg cggcgggtgt gccagccggt gctggttgga
     1921 tcgagaatcg gccgtcgccc ccttgtcgtt tgtcgacact aggcttgtgg cagtaattac
     1981 accaatgtgc cggtgctgtc aaggtccgat gagtgtgcgg tctttcgtat tccctttaga
     2041 tcggtcagga ccggggactt taccggagca agggaacgtt tctgccttgt gtccaggtca
     2101 tcccgttgtc agtcagtggc gagttgccct ggcaggctcg aagctatggc gcgtcgcatg
     2161 ccgtctatgt cgtgtttttc gagcctttgt tgggccttct ttgcatccgc ctcctcgata
     2221 gcggcgagta tttgcacgtg ttcaccgaaa gatctgggta ccgtgccctc ggctaagaac
     2281 atgcgtcgga gaaagatctt cagcggacct tcgatctgag cgtacagccg acgaaccaga
     2341 ccgctgcgag acacgctgat aatagatctg tggaacagga agttcagctc gcggtagcgt
     2401 attggatcgt cctgtaccgt ctccatctca cggatgatag gttcgagggc agcgagacac
     2461 gcgcctacta tctccggact ctcacagatg cgctctaggg c
//
LOCUS       DATMVF010000041        24384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_373430, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000041 DATMVF010000000
VERSION     DATMVF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 24384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24384
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373430"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VN178_01670"
     CDS             <1..296
                     /locus_tag="VN178_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79702.1"
                     /translation="QAADRLESPTALQLRLFQTMGEIAVNQNSTIILPVPIDLLRPFL
                     DSTDGTPESGARAARREEEEEAERLYEEAVGDATREVSSEEVSTEMPDGEANR"
     gene            538..678
                     /locus_tag="VN178_01675"
     CDS             538..678
                     /locus_tag="VN178_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79703.1"
                     /translation="MSGRTFRTLPTDGKRTARPRRRVDFTDEPADLKLRLKRPEQADR
                     ET"
     gene            858..1496
                     /locus_tag="VN178_01680"
     CDS             858..1496
                     /locus_tag="VN178_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS79704.1"
                     /translation="MSEPRRDEVRDLVVRVRREQIIGAATRVFAEKGFRRATTREVAR
                     AAGVSEGTIYNYFEDKDALLLAILDRLNETERRAADFQEGMATDFRDFLGRYLRRRMF
                     LIWENREVFRVVLSEILVNAELRKRYLQHVVDPTMRIAEENFRLRMEHGEVRYMDAPL
                     AMRSVAGAVLGVLVLGLLGDEEIGSRSDEVPDVLAGLLIHGLGAGEEDRCGK"
     gene            1486..2733
                     /locus_tag="VN178_01685"
     CDS             1486..2733
                     /locus_tag="VN178_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWS79705.1"
                     /translation="MESSVKGASPDEIVAPDLASPTFKANPYPFYARLRAEAPVWRAT
                     LRDRRTAWLVTRYEDVARVLKDDAFAKDKLNAMDLEQRAKTPWIPGFLKPLERNMLDL
                     DDPDHARLRALVSKAFTPRLIERLRGRIETLCEELLDAMKRQRERKSGTDLVVGYALP
                     LPATVIAELLGVPVEDKAKFHRWSNRLVSVSSGRDMLSALPAALSLVRYLRRLVERRR
                     ADPQDDLITALIRAEEADDRLSEDELLAMAFLLLVAGHETTVNLIASGTLALLEHPQQ
                     TERLRRDPSLAKPAVEELLRYTSSVEMATERYAREDTQIAGTTIPRGELVLAVLGSAN
                     RDERHFEDPETLDLARDPNRHLAFGRGGVHHCLGAPLARMEGQIALNAFLRRFPGARL
                     APETLRWRRGLFLRGLERLPLVL"
     gene            complement(2876..3325)
                     /locus_tag="VN178_01690"
     CDS             complement(2876..3325)
                     /locus_tag="VN178_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS79706.1"
                     /translation="MSIFPTKILLATDGSEEARLAAQAAAELSKETGSEVHVTYVLPS
                     PTELRGHHYYSEDVMRSILAQAEGEANSFLEEQAEQIRASGGKVAETHLRAGRPDKEI
                     VRLSEELEAGTIVIGSRGLGAVKRALMGGVSESVIRHAHCPVFVVRE"
     gene            complement(3595..5067)
                     /locus_tag="VN178_01695"
     CDS             complement(3595..5067)
                     /locus_tag="VN178_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWS79707.1"
                     /translation="MTETVSSKASADGWNEPTKNLPPLEVRDLPEPLPLGKIIGASVI
                     LLATALGSGELLIWPYITTQAGVGLLWLATVGFLAQYFLNMEIERYTLATGETAVTGF
                     SRFWKPWGIVFALGAILPNAFPGWASSAATSLTFLTGWGESTIPWIVTIILVSIGLAI
                     TVSPVVYNTIERVEMVLVVIIVAFLALAIVVATNLSAWTGIVTEAPQGIANFPRYVNE
                     IGIATILGAIAFAGAGGANNLTQSNYIRDKGMGMGVHIPRIVSPITGEEEARPSTGYS
                     FEVNEENLRRWDGWWKVANREQLLTFVVIGLGTLIGLSVLVASVLGEGTYGDPGSIDF
                     LQEEARVLGQEIGVWFEYFFYIACFAVLLSTNIGIVDYVSRLTADSLKTGYLARSEFW
                     SESKLYFVAAWIMCIGGSAIIWSGIEPLWLLVIASSGGGVVMFLYSGMLIWLNRRILP
                     EPIKIRGARLVVISITFAVFAVLSIYLVYWYVNAALTGQL"
     gene            5293..6069
                     /locus_tag="VN178_01700"
     CDS             5293..6069
                     /locus_tag="VN178_01700"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS79708.1"
                     /translation="MTEATAQGLGRLRGKAAVVTGAAKGIGKATAELFAREGARLVIT
                     DVDEATLSLVRGRLAGAGAEVEAVVGDVSVVEDARRMISTAVESFGRIDVLVANAGII
                     PLNTIVEATPEDWDRVMAVDGRGMFLTCKFAVEAMLEQEEPGGSIVCLSSISGLAGQA
                     QQSTYGPAKFVASGITKHLAVEWAASGIRVNAVAPGTIATEAVVELPEEYIAPMREAH
                     PVGRLGEPAEVANAILFLASDEASFVTGAILSVDGGYMAR"
     gene            6259..7452
                     /locus_tag="VN178_01705"
     CDS             6259..7452
                     /locus_tag="VN178_01705"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HWS79709.1"
                     /translation="MLIAVIADDLTGAADTGVQFVHAGYRTAVFFRATEVIEDNLDAV
                     VFDTDSRAMPAGFAAKRVVDAAHAARGARIVYKKLDSTLRGNVAAELAAALGGARRDR
                     AIVAPAFPAAGRATVGGIQRVHGVPVDETEMANDPHIPVREAHVPSLLADAFSSVGLL
                     SVEDLAEPDLVRSTLEDYDCVVADAERDADLEALVRAVPDPAGLLWVGSAGLALALGS
                     VYPGPYAGTTRVRRTPVRPVLVVVGSLSGVAREQVRRLVEAYGEVDVPVDSRAPNAVR
                     EAVGAARRALAGGTCAVVHSPEERSASGESVLGSLSEVAALLSEEGLFEGLVLTGGAT
                     AVGVAQRLGASGIRLEVEVETGVPMGLLAGPRPYPVVTKAGGFGRPDTLVGAVEALSM
                     EEWNP"
     gene            7449..8459
                     /gene="pdxA"
                     /locus_tag="VN178_01710"
     CDS             7449..8459
                     /gene="pdxA"
                     /locus_tag="VN178_01710"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWS79710.1"
                     /translation="MTQPLIAVTMGDPAGIGPEIIARAFDGDDFQEENRAVVVGDAGI
                     LERATRLLDLALRANVIAEPEDARFEPGTVDVISETELPGDLPFGTLDARAGDAAFRY
                     VERATGLASAGRVGAIATAPLNKEAMHLAGHEYPGHTEILAELTGTKDYAMMLVTDEL
                     RVIHVSTHVSLREAIERARPERELAVIRLAHESLKRLGVESPRVAVAGLNPHAGENGL
                     FGTEDAEQIAPAVAAAVEEGIDATGPHPPDTVMMRARAGAFDIVVVQYHDQGHIPIKL
                     MGFDTGVNVTVGLPFFRTSVDHGTAFDIAGTGRADAASLRAALELARTLAGAEERTEE
                     RS"
     gene            8462..9712
                     /locus_tag="VN178_01715"
     CDS             8462..9712
                     /locus_tag="VN178_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWS79711.1"
                     /translation="MKFPKVVKVRQRFPRPRVESVEAELREQLGREEIASTIRPGMSV
                     ALTAGSRGIAEIDGIVRCLVTILKEMEAEPFIVPAMGSHGGATAEGQVEILESLGITE
                     ELCGAPIRSSMEVVELGETERGVPVYMDRIASEADGVVLVNRVKAHTDFRSTIESGLM
                     KMASIGLGKHEQALALHGYGVEGIRDFMVEVGDKVLESGHILFGVATVENAYDEPAIV
                     EAIPASEIHEREAELLAGYMEMMPALPVSDIDVLYVDSLGKNYSGTGMDTNVIGRFRI
                     LGVDEPEVPTVKYVIVGDISKESHGNALGVGLADLTTRRLADRIEHKAMNANVVTSTF
                     IERAKVPIVLASDEEALRTAVRCNWGVPPDETRFVRIPNTLHLEHLYVSENLLDEVLE
                     NAEAEVVGDPEDVCFDAEGYFDGF"
     gene            9774..10577
                     /locus_tag="VN178_01720"
     CDS             9774..10577
                     /locus_tag="VN178_01720"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWS79712.1"
                     /translation="MDFASLANARVVDLSLTLSERLPGTWPGHMTYAHHNWNWFAEVE
                     GPTGKTRSTDPYQTNFVVMDEHCGTHFDAPTHFVPPPGSGLYLAGPLGEQTGDRVPLE
                     ELMGPAVVVDVRFLAGEGQPGVSPFITVDHVRGWEEEHGELRAGEVVLFLTGWDRYYV
                     EGEEGERYMHGSLVTGKAPGWPAPAPETTIYLHERGVKTIGIDAPSIGSAHDGVPVHR
                     EGLGRGMRYIEILTNLDELPIRGTYFIFMPIKISGSSGGPGRAMALLPE"
     gene            10627..11865
                     /gene="glp"
                     /locus_tag="VN178_01725"
     CDS             10627..11865
                     /gene="glp"
                     /locus_tag="VN178_01725"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWS79713.1"
                     /translation="MKVQLFEKLIEYPEAERLVSESTPLLPVEKTPLEEALGLALAWD
                     LTATTDSPPFDNSAVDGYALRSQDAEAGRVFGVADEAPAGRPAKRPVGEGEAIKIFTG
                     GVIPEGADAVVMVENTSGWGEEFELKKVPSPGDNIRAAGEDTRKGDVILPAGTEIGAP
                     EIALAATQGYGTLPAYRRPKVVVLSTGTELVEPGTRGLDPGEIFDSNSYAIVAAAREA
                     GAEASRLYAASDDADTLRAAIEEALETADVVVTSGGVSVGEKDLVKSTMLDLGVEQVF
                     WGVKFKPGKPLFYGTRGETRLFGLPGNPVSAVVCFDLFVRPALMGMMGREDKSRPRVE
                     VYFEGDIVNKFGRLHAMRVSLKRTEKGWLAESVGAQGSGLVSSLTKADALALVGPASE
                     GIPAGDPVEAIVLRQEKLVS"
     gene            12077..13132
                     /locus_tag="VN178_01730"
     CDS             12077..13132
                     /locus_tag="VN178_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWS79714.1"
                     /translation="MFESDLEDNVFRRRLMKEVAPGVPVHFSFYSPSVKVQVMHPAEN
                     LPGANGRYIGDAVTIEFVPEDAAAVERVREYVWHWEGYNAFTAVRKINHLRRGVCPEG
                     VQGVLERVRGIDKRAAAKTAEVLDPGELGDLLSRGDFKTLARLPGMGEKRARAAVEFY
                     ENERDDRRFLYAANRNDRFRGKPVITELDLSEDLEEQVWEHAVRARRLGAPDPLGPEE
                     PPGHTTVRNANLMHPLPMTPSLMGRGVYYPEQIGHFADAVRRVLVEGEKLAGVGSLRI
                     QRGKLFHTTKMAGVGMKTRARVLASGLLDDEYTYENLVAIPGVTEAQARTIASAARTV
                     RGGLVPIGAARTEVRVS"
     gene            13122..14921
                     /locus_tag="VN178_01735"
     CDS             13122..14921
                     /locus_tag="VN178_01735"
                     /inference="COORDINATES: protein motif:HMM:NF005906.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HWS79715.1"
                     /translation="MSPSLYGFVKDHEPVSPEEISTGFLSLTSPNGDARALVERLVGG
                     DSRFTWEGEDLRIAPAVSLALGEAPYVVFDVETTGSAAGKGGAITEIGALKLAGGEVV
                     DQFSTLVNPGRPIDPFVVRLTGITDRMVSDAPSISEVMPSFEEFVEGCVLVGHNVHFD
                     CSFVTAARGGTPLPNEVLDTLRLARSLVPGLKRYRLSSLVSHFGVRQAPNHRALSDAA
                     ATTEVFRRLLKLLDSAGIKSVGEALAVRGGRGRIKPQKQHLASGVPHTPGVYYFVDKH
                     GTTLYVGKAKDLKARVRTYFNGGDGRRKVGRLVEEVAEVRVRETESELHALILEAREI
                     KRLLPRYNSVGRDDKASWFVRLDLGEPYPVPERVSENPPEGTLIHLGPYRSAGALDTC
                     IEALGRIFPLKRCSGDGEVCFYGQMGRCAPCMGMDKQEYREQVVDELVALLRGEGGEE
                     HLRALVRERERLAAGLEFEAAARLRDLISGIERIRLSRTVVDAEGVQAVVAPSTEPGV
                     VEVFILYAGRLIAHRGFEPGDAIGLGRFAEEVLARPQENGSGPEKEGAGEARIVAAYL
                     RRRQAVVEAVRLKEARDLLKAAERVAEAAVPLA"
     gene            15040..15717
                     /locus_tag="VN178_01740"
     CDS             15040..15717
                     /locus_tag="VN178_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS79716.1"
                     /translation="MTRKILLVDDEPSLQKMLTHALEREGFQVQVAGDGEAALEAFKT
                     FEPHLMVLDIMLPKLDGTEVIRRIRAQSDVPVIMLTARDDEIDRVVGLELGADDYVTK
                     PFAVRELVARVRAIMRRAAVPAGQRPDELTYDRLRINLPSRRVSADGEEVDLTYTEFE
                     LLVTLASSPGRVFSRSALLRRVWGDEFRDERTVDVHIRHLREKIERDPRNPEFIHTAR
                     GVGYVFR"
     gene            15704..17542
                     /locus_tag="VN178_01745"
     CDS             15704..17542
                     /locus_tag="VN178_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS79717.1"
                     /translation="MFSAKERSSQDLDFRRKVGKLLASKTGGEFRPRLSMRIWLTALF
                     VLVTAFAAITAYQIVHPILYETLNRSSEAAFKQVGDQFEDQVRRLRERGESVTEQRIN
                     AFARSHGLQWGIVRAEDGQQTQGDLENWSSGPVRRAVEEPRRPSDDMEPIETGVHKGQ
                     LQATYAYPINVDVKGKAEPQPRALVFVKFFTESDIENVRAAMRNIERLALLAGALALL
                     IAGVAGYFAAVLISRRVSRLGLAAEHLADGNFDARITTRVEDEVGSLGATFNAMAVSL
                     KGAFDQIEQEKERGSAILDGMTDAVVGVDRDLNPVFLNPRARKLLESSDHAFHNRLQE
                     VLAKTRYSGPVTESGAKAGDRIIEIRAAPLEDGALAILRDVTEERHMQRVKAEFIANA
                     SHELKTPLTALSGYLEMLEDEEDERVRAEFLNDMRAQTVRLQSLARTLLDLSRLDANA
                     VTFRTEEVDLEDLLHGLRRDFGYTGRSLNIRAEEDVPPVRTDPNQLHRAIAILVDNAL
                     KYSDEDSPVDLGLSLQDGRVVISVTDRGCGILESEIPHIFDRFYRAQGSSRADGTGLG
                     LALAHEITDHLGGDIQVRSRPEAGSIFSVLLPLTADPPGKNGSRSP"
     gene            complement(17873..18517)
                     /locus_tag="VN178_01750"
     CDS             complement(17873..18517)
                     /locus_tag="VN178_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWS79718.1"
                     /translation="MFLSLFQQNPSAAVAFLLGLVVGITVHEASHAASAYLLGDDTAY
                     RDGRVTLNPVSHLDLLGSMMLLLAGFGWGRPTPVVPSKLRGGVFGPVAVALAGPVSNL
                     LIVVVCAVLYLLPPFQDTGGYLFILVIMVAFTNALLFVFNLIPIPPLDGSKVIFPFLP
                     RAFDGFVDFMNQYGPMILLGIILISFLTRLPILTILLAPVLPLLTLLGLPPLVF"
     gene            complement(18666..19367)
                     /locus_tag="VN178_01755"
     CDS             complement(18666..19367)
                     /locus_tag="VN178_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79719.1"
                     /translation="MKRGEVYRRRRIMAVSLLVLGAVAMVFAVFVQARASNTAQRAVP
                     IDPNNAGPATVLAEASGISISTPIRPASLTGIGYHPEGESLAEMEPRGKNLSANVVLG
                     LIFRGETPEGIHYYVMEAAGRGGPQTGAVDVGATTGTTVYTPVTGTVTAIRPDPMVQN
                     ANVIEIKPDDNPDVRVSVALVHSDGEAGVNDNVTAGKTELGTVADSAEVLDPQLSSYT
                     TDAGNHVTVTVSESG"
     gene            19621..20790
                     /locus_tag="VN178_01760"
     CDS             19621..20790
                     /locus_tag="VN178_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWS79720.1"
                     /translation="MSARKLVVSTYLQYIVLAVGVLLLVFFVRQVSGVLLTFLAAGVL
                     AYALNPIIRRLEGLGVPRVIAVIGVFLVLIVVVVVTLLLIIIPAVGQVQIVVRNPQDL
                     VDQITAQVNRLQDIPYVGRYVAGMDQDRILQLLRENAPSAEQVANVATGVIGGVFGVF
                     GTLFNLLLLVLVSVYLLLEREKITRALLGTIPETVRDQSLELFYEVEHTLVRYLRGQI
                     FLCAIMGVIGAAIMYFTVGKYALPVGAWVAATEIIPVIGAFLGAVPAVAIALFVPGGG
                     FLDALLVAGLFLIAQQLEGNVLVPRIQGGSVGVHPLWVLFATLAGTALYGLVGAVFAV
                     PVVAIIAATLRYLRGTLIFERWGRPPVKPAEEEPEAGSQAAGLVPGPPGNQKEDQ"
     gene            20787..21392
                     /locus_tag="VN178_01765"
     CDS             20787..21392
                     /locus_tag="VN178_01765"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWS79721.1"
                     /translation="MTLREPHKPSFREASKDALFVILRPLVRWLSIMRVRPDTLTVVG
                     WTLALFSAVLFGLGYARIAGAVMLLGGLFDALDGAVARESKSMSSFGAFLDSTLDRLS
                     ESAVFVGLIFFYASSARPYEALLAGLAMTFSLLTSYARARAEGLGIDCKVGLLERAGR
                     VVILSVFSIAGLPTVGLGLLAAGALVTTAQRILHVRRAARG"
     gene            21376..21876
                     /locus_tag="VN178_01770"
     CDS             21376..21876
                     /locus_tag="VN178_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79722.1"
                     /translation="MPPADKETGRAAGDYTRKTAYEATRLSNRMKQEGHPAPLGDPTS
                     GVVLVLEEPFGPRTIEALELSLQALRLQQAYVTYESTGLLAQQIQTTEPAVLVAIGAG
                     AARDIDAIDYPLVRQPFSDAQPGVWFSWTKGTIGLLTPALAPALDDDAAKRRFWLAFL
                     TLRDLT"
     gene            22179..23927
                     /locus_tag="VN178_01775"
     CDS             22179..23927
                     /locus_tag="VN178_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWS79723.1"
                     /translation="MFLGLAIALVFVTSISAGAEPDGGIRAKKADVAAAQDRLTEIRM
                     EAGSAEAAYNNALYEMNQLNGQITRTTEELEVAKERLAVAKKTMEQQATQVYKSADVA
                     FMDVLVGVDDFSEFASRLKLWVRLLDKERAEFLEVREARNELAARRDRLEAQREQRVA
                     AVETAIAQRERANEAEARASGYLDSLDAELRGAIRAAQDRQAAQARAAAAAMAEKGQE
                     PVGEPEPIPEVQVSQVKQAGVPRADLQSERAAAERKAAARATAERAERLAEKRAAERK
                     AARKAAEQAAAEREAARLAAEKVARKEAREEAQRQAELAARRAAKRQAAREEAERKAE
                     QAARRAEIEEAAAEQAAAEREAARLAAERRADRRAEQRAEERAAERAEASASASAAAE
                     EEAAGERWAEKRAKERAEASASASASVSSSKGSERGGGGRDEGASSASATASASASGT
                     PAGGGGGRVSGLGEDVLAVADDHLGTPYEFSPPGPCVAFEAEDCSCFTMLVFQEFGIA
                     LPDSPGGQMGYGTPVHGAPQAGDLLFWSEDGSGYITHVGIAMGDGTTIHAQVHNEVTI
                     TGINHIAGYVGARRLL"
     gene            24198..>24384
                     /locus_tag="VN178_01780"
     CDS             24198..>24384
                     /locus_tag="VN178_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWS79724.1"
                     /translation="MWPLVDMMVKIDLRTMTMDVPPQDVITRDNVPARVNAVVYFRVT
                     DPNRSVIEVENHVLATSQ"
BASE COUNT         4548 a         7509 c         8109 g         4218 t
ORIGIN      
        1 ggcaggccgc cgaccgtctg gagagcccca ctgctttgca gctgaggttg ttccagacga
       61 tgggggagat cgccgtcaac cagaactcca ccatcatcct tcccgtcccc atagacctcc
      121 taaggccctt cctggattcc actgacggca ctcccgaaag cggagcgagg gcggcacggc
      181 gcgaggaaga ggaagaggcc gagcgcctct acgaggaggc cgtaggagac gctacgcgcg
      241 aggtgtcgag cgaggaggtg tccaccgaga tgccagatgg ggaagccaac cgataggagc
      301 ctctcaaaac cagatagaga aaccatcgag gagcaggtca ggttgatctt gccagatgat
      361 gcctactacg agtatttcgt taccgcccga tggtaactat gagctgcctg attgcgctag
      421 atgccttgga aatttctgta ccccgctcgg aggagaagtt ggccgaaacc gtcgacgcat
      481 ggcatgctcc ttcgcctctg tacgtgctgt cacgacgctc gctcgaagag tgctctgttg
      541 agcggtagaa cgttcaggac gttgccgacg gatgggaaac ggacggccag gccaaggcgg
      601 cgcgtcgact tcacggacga gccggccgac ctgaagctgc ggctgaagcg gcccgagcag
      661 gccgaccgcg agacgtgaag aagcctcagc tgttcccttc ccctatcact ccacgtagct
      721 caggctgttc atgatagtaa acctcggtta aaccaagctg tttaaagcca tgaaactcct
      781 tgatgaagtg cgcagtgaga cgtatacgga gtaagtactc agcataatga gcacttgctc
      841 aacaggaggt ctgagcggtg tccgagccac ggagagatga ggtgcgtgat ctggtcgtcc
      901 gtgtccgacg ggagcagatc atcggggcgg ccacgcgggt cttcgccgag aaggggttcc
      961 gccgcgctac cacgagggag gtggcgcgtg cggctggcgt gtcggaaggg actatctaca
     1021 actacttcga ggacaaggac gccctactgc tagcgatact cgacaggttg aatgagacgg
     1081 agcggcgggc agcggacttc caggagggga tggccacgga ttttcgtgat ttcctggggc
     1141 gatacttgcg ccggcgcatg tttttgattt gggagaatcg ggaggtcttt cgggtcgtcc
     1201 tctcggagat actcgtgaac gccgaattgc gcaaacgcta ccttcagcac gtagtggacc
     1261 cgactatgag gattgccgag gagaacttcc ggttgagaat ggaacacggc gaggtgcgat
     1321 atatggacgc gcccctggcg atgcggtccg tggccggggc tgtgctaggg gtcctggtcc
     1381 tgggtctact aggcgacgag gagataggtt cacggtcgga cgaggtgccc gacgtcctcg
     1441 ccgggctact gatccacggc ctcggtgcgg gtgaagagga tcggtgtgga aagtagcgtg
     1501 aaaggagcca gcccggacga gatcgtcgcg cccgacctcg ccagcccgac tttcaaggcc
     1561 aatccctatc cgttctacgc gcgccttcgc gcggaagccc ccgtttggcg cgcgaccctt
     1621 cgcgaccggc ggacggcgtg gctggtcacc cgctacgagg acgtggcaag ggtgttgaag
     1681 gacgacgcgt tcgccaagga taagctgaac gctatggatc tggagcagcg ggcgaaaacg
     1741 ccgtggattc cgggattcct caagccgctc gaacgcaaca tgctcgacct ggacgaccct
     1801 gaccacgcgc ggctgcgggc cctcgtttcc aaggcgttca cccctcgtct aatcgaacgg
     1861 ctacgggggc ggatcgagac gctctgcgaa gaactcctcg atgccatgaa gcgccaaagg
     1921 gaacgtaaaa gcgggactga cctggtcgtc ggctacgcgc tgcccctgcc cgctaccgtg
     1981 atcgccgaac tgttgggtgt accggtggaa gacaaggcca agttccaccg gtggtcgaac
     2041 agattggtct cggtctcctc gggtcgagac atgttgagtg ccctccccgc cgcgctttcg
     2101 ttggtgcgct atctccgcag actcgtggag cggcgtcgtg ccgaccccca ggacgatctc
     2161 atcacggcgc tgatccgggc tgaggaggca gacgacaggc tgagcgagga cgagctcctg
     2221 gccatggcat tcctgctcct ggtggccggc cacgaaacca ccgtcaacct gatcgccagt
     2281 ggcacgctcg ccctcctcga gcatccacag cagacggagc ggctcaggcg cgacccttcg
     2341 ctcgcgaagc cggcggtcga ggagctcttg cgctacacca gctctgtgga gatggcgacc
     2401 gaacgctatg cgcgagagga tacgcagatc gctggcacga cgataccacg cggcgaactg
     2461 gttctggcgg tgttaggttc ggccaaccgc gacgagcggc acttcgagga ccccgaaacc
     2521 ctcgacctcg cgcgcgatcc gaacaggcat ctcgccttcg ggcggggcgg cgtgcaccac
     2581 tgcctgggag caccgctcgc ccgcatggaa gggcagatcg ccttgaacgc ctttctgcgg
     2641 cgcttccctg gcgcgcgcct ggcgcccgaa accctgcgct ggcggcgcgg gttgttccta
     2701 cgcgggctgg agaggctgcc gctcgttctc taaggcaaac gatctcggcg cccaggatgc
     2761 ctcccctgac ggttcataca agcatcccga ttgcgcatct ctcgagcgtc caacgcctcc
     2821 ctcggatgac aaagccaggc cgtgggcaat gttccggtcg tcggacggtc gtttcttact
     2881 cgcgcaccac gaacacgggg cagtgggcgt gcctgatcac cgactccgaa acaccgccca
     2941 tcagcgccct ctttacggcg cccagtcctc tgcttcctat cacgatcgtc cctgcttcca
     3001 gttcctcact cagcctcacg atctccttgt ccggccttcc cgccctgagg tgcgtctcgg
     3061 ccaccttacc ccctgacgcc cggatctgct cggcctgctc ctcgaggaac gagttggcct
     3121 ccccctcggc ttgcgcgagg atcgaccgca tgacgtcctc ggagtagtag tggtgtcccc
     3181 tgagctccgt aggcgacggc agcacgtagg taacgtgtac ctccgagccg gtttccttgg
     3241 agagctcggc ggccgcctgt gcggccagac gggcctcctc ggaaccgtcg gtggccagaa
     3301 ggatcttggt ggggaatata ctcatcgggg taacctcctt atacttcgca ttctccttga
     3361 ctaccatcat gccacttgaa aaaacccggc gcatccgccc agggccgttc accgggtcaa
     3421 ggacggagtg gagagcaggt tgcccgggtt gccctcggat ctaggagagc aacccgagca
     3481 gatacgcgat cgtgatcagg acgcagaaga tcaccatggc cagcaaggtg gttatcctga
     3541 gccgcttgaa cactcctgct gcgccctctc acccgctaat tctcaccgtg cccgctacag
     3601 ttgtcccgtc agggcggcgt tgacgtacca gtagaccagg tatatcgaga gcaccgcgaa
     3661 cacagcgaac gttatggaga tcactacaag gcgtgcgccc ctgatcttga tgggttcggg
     3721 caggatgcgg cggttgagcc agatgagcat acccgagtag aggaacatca cgaccccgcc
     3781 tccagaggaa gcgatgacca gcaaccatag tggctcgatg cccgaccaaa tgatcgctga
     3841 gcccccgatg cacatgatcc acgcggccac gaagtagagc ttgctctcgc tccagaactc
     3901 gctcctggcc aggtagccgg tcttgagcga gtcggcggtg agcctggaga cgtagtccac
     3961 gatgcctatg ttggtcgaga gcaggacggc gaagcaggct atgtagaaga agtactcgaa
     4021 ccagacgcct atctcctggc cgagcaccct cgcctcttcc tgcaagaagt cgatgctccc
     4081 tggatccccg taggtcccct cgccaaggac cgaggcgacc agcacggaga ggccgatgag
     4141 cgtacccaac cctatgacga cgaaggtcag gagctgttcc ctgttggcga ccttccacca
     4201 gccgtcccag cggcgcaagt tctcctcgtt cacctcgaac gagtacccgg tggacggcct
     4261 ggcctcctct tctccggtga tcggggagac gatgcgcggg atatggaccc ccatacccat
     4321 ccctttgtcc ctgatgtagt tgctctgagt gaggttgttg gcgccgccgg cgccggcgaa
     4381 ggctatcgcc cccaggatcg tcgcgatccc gatctcgttt acgtacctgg ggaagttggc
     4441 gatgccctgt ggtgcctcgg tgacgatccc cgtccaggcc gatagattgg tggcaacgac
     4501 tatcgccagt gccaggaagg caacgatgat gacgaccagc accatctcga ccctctcgat
     4561 ggtgttgtag acgaccgggg agacggttat cgctaggccg atggagacga ggattatcgt
     4621 gactatccag ggaatcgtgc tctcacccca accggtgagg aacgtcagcg acgtggcggc
     4681 gctcgacgcc catccgggga aagcgttcgg caagatcgcg ccgagcgcaa acactatccc
     4741 ccagggtttc cagaaacggc tgaaaccggt cacggcggtc tcgccggtgg ccagggtgta
     4801 gcgttcgatc tccatgttga ggaaatactg cgccaggaaa cccacggtcg ccagccacag
     4861 cagcccaact cccgcctgag tggtgatgta gggccagatc agcaactcgc cggaaccgag
     4921 cgccgtggcg agcaggatca cgctcgcccc tatgatcttg cccagcggca agggctccgg
     4981 caggtccctg acttcaagcg gaggcaggtt cttggtcggc tcgttccaac catcggccga
     5041 cgccttgctg ctcacggtct cggtcatgcc tatattcctc cttgtctccc taaccttgcg
     5101 gcgcatgtta ttggaatcgc tacatcgtgt caatcggcac cgccaaggtg tctttatccc
     5161 ttccaacgac gtgtcgcgag cggggtttga cgggccgagt atcacggtga taggttgtgg
     5221 aggataggtg aagtttatgt cagaaattcc gggtaacgtc gtgcccgcgg cgagtgcggc
     5281 ggaggaggta gcgtgacgga ggcgacagcg cagggactcg gcaggttgcg tgggaaggcg
     5341 gccgtagtca cgggagccgc gaagggtatc ggaaaggcga ccgccgagct cttcgccagg
     5401 gagggggcgc gcctcgtcat caccgatgtg gacgaggcca ctctctccct ggtgcgcggg
     5461 cggctggccg gggctggcgc ggaggtcgaa gccgtcgtcg gggacgtctc tgtcgtggag
     5521 gatgcgcggc gcatgataag cacggctgtc gagagcttcg ggcgcataga cgtgctcgtg
     5581 gccaacgccg gcatcatccc gctgaatacc atcgtggagg ctacgcctga ggactgggac
     5641 cgtgtgatgg ccgtggacgg gcggggcatg ttcctcacct gcaagttcgc cgtcgaggcc
     5701 atgctggagc aggaggagcc ggggggttcc attgtctgcc tgtcctcgat ctcgggtctc
     5761 gccggacagg cccagcagtc cacctacggg cccgccaagt tcgtcgcctc ggggatcacc
     5821 aagcacctcg ccgtcgaatg ggccgcaagc ggcatccgcg tcaacgccgt ggcgccgggc
     5881 acgatagcga ccgaggccgt cgtggaactg cccgaggagt acatcgcccc gatgagggag
     5941 gcccacccgg tagggaggct cggcgaaccg gccgaggtgg cgaacgccat cctgttctta
     6001 gcctcggacg aggcctcgtt cgtgacgggc gcgatcctct ccgtggacgg tgggtatatg
     6061 gcgcgatgaa gcatgtcagc acgccgcctt tggcggcttg tcagcatttc agctggtcag
     6121 ctacgtgtag tgtggtgaga cgttcgtgcg cttgagagaa tgtcagagga gcaagtactc
     6181 tttcagagtc ttctctgtcc gtaaacacct gacatgctga agtgctgaca agcggaggcg
     6241 aagccggagc gtgctgaagt gctgatcgct gtaatcgccg acgatctgac cggcgcagcc
     6301 gataccggcg tccagttcgt ccacgccggt taccggacag cggtgttttt ccgcgcgacc
     6361 gaggtgatcg aagataacct cgacgccgta gttttcgata ccgattcccg tgccatgcca
     6421 gcaggcttcg cggcgaagcg cgttgtggat gccgcgcacg cggcccgtgg ggcacgcatc
     6481 gtgtacaaga agctcgactc cactttgcgg gggaacgtcg ccgcagagct cgccgcggcg
     6541 ttgggcggtg cgcgtcgaga tcgtgccatc gtcgcgcccg cctttccggc cgccgggcgt
     6601 gccacggtcg gtggcatcca acgcgtccac ggcgtccccg tcgatgagac cgagatggcg
     6661 aatgatcccc atattcccgt cagggaggcc cacgttccga gcctgctcgc cgatgcgttc
     6721 tcgtcggtcg gcctattgag cgtcgaggac ctcgctgaac ctgatctcgt tcgcagtact
     6781 ctggaagatt acgattgcgt cgtcgccgac gcagagcgcg acgctgatct ggaggctctt
     6841 gtgcgggccg tgccagaccc cgccgggtta ctctgggtag gctctgccgg gctcgcgctc
     6901 gcgctgggga gcgtctaccc tgggccctat gcgggaacca cgcgtgtacg aagaactccg
     6961 gttcgccccg tactcgtggt cgtcggtagc ctgagcggcg tggcgcggga gcaggtgcgg
     7021 cggctggtcg aagcgtacgg ggaggtagat gtccctgtcg atagtagagc gcccaacgcc
     7081 gtgcgagagg ctgtgggcgc tgccaggagg gctcttgccg gtggaacgtg cgccgtcgtt
     7141 cactcgcccg aggagagaag cgcttccggc gaatcggtcc tcgggtcgct gtccgaggtg
     7201 gccgcgcttc tctcggaaga ggggctattc gaagggctgg tcttgaccgg gggggcaacg
     7261 gctgtcggtg ttgcgcagcg gctcggggct tccgggatca ggctcgaagt agaggtcgag
     7321 accggggtcc caatgggcct cctggccgga cccagacctt accccgttgt caccaaggca
     7381 ggaggcttcg gcagaccgga tacactcgta ggagcagtgg aagcgctcag catggaggag
     7441 tggaacccat gacacagccc ctcatagccg tgacgatggg ggacccggcc gggatcgggc
     7501 ccgagatcat cgcccgagcc ttcgacggag acgacttcca ggaagagaac cgtgccgtcg
     7561 ttgtaggtga cgctgggatt ctggagcggg caacgcgcct gttggatctg gcgctgcgcg
     7621 ctaacgtcat cgcggaaccc gaggatgcga ggttcgagcc aggcaccgtt gatgtgatct
     7681 ccgagaccga actgcccggg gatctaccct tcggtaccct cgacgcgagg gccggtgacg
     7741 cggccttccg ctacgtcgag cgggcgaccg ggctggcgag tgcgggacgc gtcggggcga
     7801 tagcgacagc gccgctcaac aaggaggcca tgcacctggc aggccacgaa tatcccgggc
     7861 acaccgagat cctggccgaa ctgacgggca cgaaagacta cgctatgatg ctcgtcacag
     7921 acgagctgag agtgattcac gtctccaccc acgtctcctt gagggaggcc atagagcggg
     7981 ccaggcccga acgagagctg gccgttatcc ggcttgccca cgagtcgttg aaaagactgg
     8041 gcgtcgagag cccgagggtg gccgtcgccg gcctgaatcc ccacgccggg gagaacgggc
     8101 tcttcgggac cgaggacgcc gagcagatag cgccggccgt ggccgccgcc gtggaggagg
     8161 gcatcgacgc gacgggcccg catccgcccg acacagtgat gatgcgggcc agggcgggcg
     8221 cgttcgacat cgtcgtcgtc caataccacg accagggaca cattcccata aaactgatgg
     8281 gcttcgacac cggggtaaac gtgacggttg gactgccgtt cttcaggacg agcgtggacc
     8341 acggcacggc cttcgacatt gcggggacgg gacgggccga cgcggcgagc ctccgcgccg
     8401 ccctcgaact cgcccgcacc ctggccgggg ccgaggagcg gacggaagag aggagttaga
     8461 ggtgaagttt cctaaggttg tcaaggtcag gcagaggttt ccgaggccgc gcgtcgagag
     8521 cgtcgaggct gagctgcggg agcagttggg tcgggaggag atagcttcga ccataaggcc
     8581 aggtatgagc gtggcgctca cggcggggag ccggggcatc gctgagatcg atgggattgt
     8641 gcgctgtctc gtcacgatct tgaaggagat ggaagcggag ccgtttatcg tgcctgcgat
     8701 gggcagccac ggtggggcta cggcagaggg gcaggtcgag atcctcgagt ctctgggcat
     8761 cacggaagag ctctgcgggg cgccgatacg ctcctcgatg gaggtcgtgg aactcgggga
     8821 gacggagcgc ggggtacccg tatacatgga cagaatagcc tccgaggccg acggcgtggt
     8881 tctcgtcaac agggtcaaag cccacactga cttccgctcc accatagaga gcggcctcat
     8941 gaagatggcc tccatcgggc tcggaaagca cgaacaggcg ctcgcgctgc acggttacgg
     9001 cgtcgagggt atccgcgact tcatggtcga ggtgggggac aaggtcctcg agtccggcca
     9061 catcctcttc ggcgtggcga ccgtcgagaa cgcctacgac gagcccgcca tcgtcgaggc
     9121 gatacccgcc tcagagatcc acgagcgcga ggccgagctc ctcgccgggt acatggagat
     9181 gatgcctgcg ctgcccgtct cggacataga cgtcctctac gtcgactctc taggcaagaa
     9241 ctactcggga accggcatgg acacgaacgt gatagggcgc ttccgcatcc tcggcgtcga
     9301 tgagccggag gtgccaaccg tcaagtacgt aatcgtcggc gacatcagca aagagtcgca
     9361 cggcaacgcg ctcggggtcg gtctcgccga cctgaccacg cggcgcctcg cggaccggat
     9421 cgaacacaag gccatgaacg ccaacgtcgt caccagcacc tttatcgagc gggcgaaggt
     9481 gcccatcgtc ctcgcgagcg acgaggaggc gctccggacg gccgtccgct gcaactgggg
     9541 cgtcccaccg gatgagacgc gcttcgtccg tatcccgaac actttgcacc tcgagcacct
     9601 gtacgtctcc gagaacctcc tcgacgaggt gctggagaac gccgaggccg aggtagtcgg
     9661 cgaccctgaa gacgtgtgtt tcgacgccga aggttatttc gacgggtttt gagaggttcg
     9721 cgaatgccca gcgtggcggc ggacgttctg tgggtaccgg gtaaaggagg agtatggact
     9781 tcgcatcgct ggcgaatgcg agggtcgtgg atctctcgct gaccttatcg gagcggctgc
     9841 ccggtacgtg gccggggcac atgacctacg cccaccacaa ctggaactgg ttcgccgagg
     9901 tagaaggccc aacgggcaag accaggagca cggaccccta ccagaccaac ttcgtcgtca
     9961 tggatgagca ctgcggaacc cacttcgacg ccccgaccca cttcgtccca ccccccggtt
    10021 cgggcctgta tctcgccggg cccctcgggg aacaaacagg ggacagagtg ccgctcgaag
    10081 aactgatggg tcccgcggtc gtcgtggacg tgcgttttct agccggagag ggccaacccg
    10141 gcgtgagccc tttcatcacc gtcgaccacg tccgcggctg ggaggaggag catggagagc
    10201 tccgggccgg cgaggtcgtg ctcttcttga cgggctggga ccgctactac gtcgagggcg
    10261 aagagggcga gagatacatg cacggctcgc tcgtcacggg taaggctcca ggctggcctg
    10321 ctcccgctcc cgagaccacc atctaccttc acgagcgcgg cgtgaagacc ataggcatcg
    10381 acgcccccag catcggaagc gcccacgacg gcgtgcccgt tcaccgggag ggactgggcc
    10441 gcgggatgcg ctacatcgag atcctgacca accttgacga gttgccgata cgtgggacct
    10501 acttcatctt catgccgatc aagatctcag gctcttccgg tggcccaggc cgggcgatgg
    10561 cgctgctgcc agagtagaga actgtatggt agcgtgtcgg caagcaaacg ataggttagt
    10621 cgaggtgtga aggtgcaact tttcgagaag ctgatcgagt atcccgaggc cgagcggctg
    10681 gtgtccgaga gcacgcctct gctccccgtc gaaaagacgc cgctagagga ggctctgggc
    10741 ctcgcgctcg catgggatct tacggccacc accgactcgc cgcccttcga caactcggcg
    10801 gtggacgggt acgcactccg tagccaggat gccgaagccg gacgcgtctt cggcgtggcc
    10861 gacgaggcgc cggcgggccg tccggcgaag aggcccgtcg gagaaggcga agctatcaag
    10921 atcttcaccg gcggcgtcat ccccgagggg gccgacgccg tggtgatggt cgagaacacc
    10981 tcgggctggg gcgaggaatt cgaactgaag aaggttcctt ctcccggcga caacatccgc
    11041 gcggcgggtg aggacacacg caagggtgac gtcatcttgc ccgcaggaac cgagattggg
    11101 gcccccgaga tcgccctcgc cgcgacgcag ggctacggca cgctccctgc ctatcgcagg
    11161 ccgaaggtgg tcgttctatc gacggggacc gaactcgtcg agccggggac gcggggcctc
    11221 gacccgggtg agatcttcga ctcaaactct tacgccatcg tcgccgcggc ccgcgaggcc
    11281 ggcgccgagg ccagccgcct ctacgccgcc tcggacgatg cagataccct tcgggcggcg
    11341 atagaggagg cgctggagac ggccgacgtg gtcgtgacca gcggcggggt ctcggtgggt
    11401 gagaaggacc tcgtcaagag cacgatgctg gacctcgggg tggagcaggt cttctggggc
    11461 gtcaagttca aacccggcaa gccacttttc tacggcacgc gcggcgagac ccgcctgttc
    11521 ggccttcccg gcaacccggt ctccgcggtg gtctgcttcg acctcttcgt caggccggct
    11581 ttgatgggta tgatgggccg cgaggacaag agcaggcctc gcgtcgaggt ctacttcgag
    11641 ggggacatag tcaacaagtt cggcaggctg cacgccatgc gcgtcagcct gaagagaacg
    11701 gagaaaggct ggctggcaga gtccgtcggg gcccagggct cgggcctcgt cagctcgctg
    11761 acgaaagccg acgccctcgc cctcgtcggc ccggcctcgg agggcattcc cgccggagat
    11821 cccgtcgagg ccatagtgct gcggcaggaa aagcttgtca gctagtaagc atttcagcct
    11881 gtcagcacgc gccctatcgg gcgcttgtca gcacttcagc tttcgggtgc gcaaggcctg
    11941 gctaacttgc tgacaaggcg gcgcagccgc cgtgctgact tgctgacaag cgagggccgc
    12001 aaggcccgaa gcgtgctgaa atgctgacaa gcggaggcga agccggagcg tgctgacatg
    12061 ctagaattac gatccgatgt tcgagtcaga cctcgaagat aacgtcttcc gcaggcggct
    12121 gatgaaggag gtggcaccgg gggtcccggt gcacttctct ttttattcgc cctcggtcaa
    12181 ggtacaggtg atgcaccccg cggagaatct tccgggggcc aacggccgct acatcgggga
    12241 cgccgtcacc atcgagttcg tccctgagga cgccgcagcc gtcgagcggg tgcgcgagta
    12301 cgtctggcac tgggagggct acaacgcctt cacggcggtg cgcaagatca accacctcag
    12361 gcgcggcgtc tgccccgagg gtgtgcaggg cgtcctggaa agggtccgcg gcatagacaa
    12421 gagggcggcc gcgaaaacgg cggaggtctt ggatcctggg gagttggggg acctcctctc
    12481 caggggggac ttcaagacgc tggcacggct gccgggcatg ggcgagaaac gggcccgcgc
    12541 cgccgttgag ttctacgaga acgagaggga cgaccggcgc tttctgtatg ccgccaatcg
    12601 taacgaccgc ttcaggggca agccggtgat cacggagctg gacctctcgg aagatctcga
    12661 ggagcaggtg tgggaacacg ccgtgcgggc gcgaagactg ggcgccccag acccgctcgg
    12721 gccagaggag cctcccggcc acaccaccgt gcgcaacgcg aacctcatgc acccgctccc
    12781 gatgacgcca tccctgatgg gccggggcgt ctactacccc gagcagatag ggcacttcgc
    12841 cgacgcggtg agacgtgtcc tcgtagaagg ggagaagctg gccggtgtcg gatccctacg
    12901 catccagcgc ggcaagcttt tccacaccac caagatggcc ggcgtcggga tgaagacgcg
    12961 ggccagggtc cttgcgagcg gactgctcga cgacgagtac acctacgaaa acctcgtcgc
    13021 catccccggc gtcacggagg cacaggccag gaccatcgcc agcgccgcga ggaccgtaag
    13081 aggggggctc gttcctatcg gagcggcgcg gacggaggtc cgtgtctcct agtctctacg
    13141 gcttcgtgaa ggaccacgag cctgtaagcc cggaagagat ctccacgggg ttcctctctc
    13201 taacgagccc gaacggtgat gccagggccc tcgtcgagag gctcgtcgga ggagattcca
    13261 ggttcacctg ggagggcgaa gacttgagga tcgcgcccgc cgtctcgctc gcgctcggtg
    13321 aggctcctta cgtcgtcttc gacgtggaga ccaccggttc tgcggccggg aaggggggcg
    13381 ccattacgga gatcggagcc ctcaaactcg cgggaggaga ggtagtagac cagttctcga
    13441 cgctcgtcaa cccggggcgc ccgatagacc ccttcgtggt acgcctgacc ggcataacgg
    13501 accgcatggt ctcggatgcc ccttctatct ccgaggtcat gccaagcttc gaggagtttg
    13561 tcgagggttg cgttctcgtc gggcacaacg tccatttcga ctgctccttc gtcacggccg
    13621 caaggggtgg caccccactc cccaacgagg tcctcgacac cctgagactc gcgcgctccc
    13681 tggtgccagg tctcaaacgc taccgtctga gctcgctcgt cagccacttc ggcgtgcgcc
    13741 aggcgccgaa ccacagggcc ctctccgacg ccgctgcgac gacggaggtg ttccgcagac
    13801 tgctgaagct tttggactcc gcgggcataa agagcgtcgg cgaagcgctc gccgtccgcg
    13861 gggggagggg ccgcatcaaa ccccagaagc agcacctggc gagcggggtg ccccatacgc
    13921 ccggcgtcta ctacttcgtc gacaagcacg gcaccaccct ctacgtcggt aaggcgaagg
    13981 atctgaaggc ccgcgtccgt acctacttca acggcggaga cggacgccgc aaggtcggac
    14041 gcctcgtcga ggaggtcgcc gaggtacgcg tcagggagac ggagagcgag cttcacgccc
    14101 tcatcctcga agccagggag ataaagcgtc tcttgccacg ctacaactcc gtaggcaggg
    14161 acgacaaggc gagctggttc gtgaggctgg atctgggcga accttacccc gtacccgaga
    14221 gggtctcaga gaacccgcct gaaggcaccc ttatccacct gggtccttac aggagcgccg
    14281 gagcgttgga cacctgcatc gaggcgctgg gtaggatctt cccgctcaag aggtgctcgg
    14341 gggatgggga ggtctgcttc tacggccaga tgggacgctg tgcgccgtgc atgggcatgg
    14401 ataagcagga ataccgcgag caggtcgtag acgagttggt cgcgctcttg cgcggcgagg
    14461 ggggcgagga gcacctcagg gcgctcgtca gggagcgcga acgtctggcc gccgggctgg
    14521 agttcgaggc cgccgccagg ctgcgagacc tgatctccgg catcgagcgg atacgcctct
    14581 ccaggacggt ggtggacgcc gagggtgtgc aggccgtcgt cgccccgtcg acggagcccg
    14641 gtgtggtgga ggtctttatc ctgtacgcgg gacgtcttat cgcccaccgc ggcttcgagc
    14701 cgggcgacgc gatcggatta gggcgcttcg ccgaagaggt gctggccagg ccccaggaga
    14761 acggctccgg tcccgagaag gaaggcgcag gcgaggcgcg aatcgtcgcg gcttatctca
    14821 ggcgccggca ggcggtggtc gaggccgtgc gcttgaaaga ggcgcgggat cttctgaagg
    14881 cggctgagcg ggtggccgag gccgcggtcc cgctggcttg aagacctttc ggatcgggag
    14941 gctttgtact ccttcatgct agaatgtaat caaggtgtat tgacggttct tgagtagtag
    15001 aagagagttt ggccggcgat ggtacaggat ttacagggtg tgacgcgcaa gatacttctg
    15061 gtcgacgatg agccgtcgct ccagaagatg ctcacgcatg ccctcgagcg cgaaggattc
    15121 caggtccagg tcgccggcga cggcgaggcc gccctggaag cgttcaagac cttcgagccg
    15181 catttgatgg tgctcgacat catgctcccc aagctcgacg gcacggaggt cataaggagg
    15241 atcagggcgc agagcgacgt gcctgtcatc atgctgaccg cccgcgacga cgagatagac
    15301 cgggtcgtcg ggctcgagct cggggcggac gactacgtca ccaagccgtt cgccgtcagg
    15361 gagctggtgg cccgcgtcag ggcgatcatg cgccgggccg cggtgccggc gggccagcga
    15421 cccgacgagt tgacctacga caggctcagg atcaacctgc cgagccggag ggtgtcggcc
    15481 gacggcgaag aggtggacct gacctacacc gagttcgaac tgcttgtgac actggcctcc
    15541 agtccgggca gggtgttctc gcgcagcgcg cttctgcgca gggtgtgggg cgacgagttc
    15601 cgcgacgaac gcacggtgga cgtccacatc cgccacctcc gcgagaagat agagcgcgac
    15661 cccagaaacc ccgaattcat ccacaccgcg cggggtgtcg gatatgtttt ccgctaaaga
    15721 acggtcgagc caggatctag acttcaggcg aaaggtcggt aaactgctcg cctcgaagac
    15781 ggggggcgag ttcaggcccc gcctgagcat gcgcatctgg ctcacggcgc tgttcgtgct
    15841 cgtaaccgcc ttcgccgcga taaccgctta ccagatcgtt catcccatcc tctacgagac
    15901 actcaaccgc tctagcgagg ccgccttcaa gcaggtcgga gaccagttcg aggaccaggt
    15961 gcggcgtttg agggaacgcg gcgaatccgt gaccgaacag cgcataaacg cgttcgcccg
    16021 ctcccacggg ttgcagtggg gcatagtcag ggccgaggac ggccaacaga cgcaagggga
    16081 cctcgagaac tggtcctcgg ggcctgtgag gcgcgccgta gaggagccca gaaggccgag
    16141 cgacgacatg gaaccgatcg agacgggcgt ccacaaggga cagttgcagg ccacttacgc
    16201 ctatcccata aacgtggatg tcaaaggcaa ggccgagcct cagcctcggg ccctggtctt
    16261 cgtcaagttc ttcacggaga gcgacatcga aaacgtgcgg gcggcgatga ggaacataga
    16321 gcgattggcc ctgctcgccg gcgccctggc gctgctcatc gccggggtcg cggggtactt
    16381 cgcggcggtt ttgatctccc ggcgcgtcag ccgcctgggg ctcgccgccg agcatctggc
    16441 cgacggcaac ttcgacgcga ggataacgac gcgcgtcgag gatgaggtcg gctctctcgg
    16501 cgcgacgttc aacgctatgg cggtctcact caaaggggcc ttcgaccaga tagaacagga
    16561 gaaggaacgc gggagcgcca tactcgacgg catgacggac gcggtagtcg gcgtggaccg
    16621 agacctgaac ccggtctttt tgaacccccg cgcgaggaag ctcctcgagt cctctgacca
    16681 tgctttccac aaccgcctgc aagaggtgct cgccaagacc cgatacagcg gtcccgtgac
    16741 agagtccggg gcgaaggcag gggacaggat catcgagatc cgggccgccc ccttggagga
    16801 cggggcgctc gccattttgc gcgacgtcac ggaggagcgg cacatgcagc gcgtcaaggc
    16861 ggagtttatc gccaacgctt cccacgagct caagacaccc ctgacggccc tctccggcta
    16921 cctggagatg ctagaggacg aggaggatga acgggtcagg gcagagttcc tgaacgacat
    16981 gcgggcccag accgtgcgcc tgcagagcct ggcgcgcacc ctgctcgacc tctcgcgcct
    17041 cgacgccaac gccgtcacct tcaggacgga ggaggtcgac ctcgaagacc tgctccacgg
    17101 gctgcgcagg gacttcggat atacgggccg ttcgctgaac atcagggccg aggaggacgt
    17161 gccgcccgta aggacagacc cgaaccagct tcacagggcc atcgccatcc tcgtggacaa
    17221 cgccctcaag tactcggatg aggactcacc cgtcgacctt ggactctccc tccaggatgg
    17281 gcgggtcgtt atcagcgtaa cggaccgtgg ttgcggcatc ctggaatccg agatacccca
    17341 catcttcgac cgtttctacc gggcccaggg ctcctcacgc gccgatggta caggcctggg
    17401 actcgccctc gcccatgaga tcaccgacca cctcggcggt gacatccagg tacgcagccg
    17461 acccgaagcc gggagcatct tctccgttct cctccccctg accgcagacc ccccaggcaa
    17521 gaacggaagc cggtctcctt agtctctacc tgaggtacag cttacgcttt agcggatttg
    17581 agagtagaat acattcgggg cggcatggcc tccgtagatt ctcccccctc cctacggacc
    17641 gtgctgcccc gttatgagca attcagcacg ctcaggcttt tagccttcgc ttgtcagccg
    17701 gcggctttgc cgccttgtca gcatgtcagc taggtctgag atggtggagg gatgcttctc
    17761 tggagaggct tcttatcttg gcaaacgatt cggtgtcgag cacagccggc gaaccgacac
    17821 caaaaagctg acatgctgac aagcgcccga cagggcgcgt gctgaaatgc tttcagaaga
    17881 ccaagggggg gagaccaaga agggtcagga gtggtagtac gggggcgagc aggatggtca
    17941 ggatggggag acgagtcagg aaagagatca ggattatgcc gagcaggatc atcggcccgt
    18001 actggttcat gaaatccacg aagccatcaa aggcgcgcgg caggaaaggg aagatcacct
    18061 tcgagccgtc gagcgggggg atcgggatga ggttgaagac gaagagcagt gcgttggtga
    18121 aggcgaccat gataacgagt atgaacaggt agccgcccgt gtcctggaag gggggaagaa
    18181 ggtaaaggac ggcgcagacg accacgatca ggaggttgga gacgggccct gcgagcgcga
    18241 cggccacggg cccgaacacc ccacctctga gcttggaggg taccaccggt gtgggcctgc
    18301 cccacccgaa gccggccagg agaagcatca tgctgccgag caggtcgagg tgcgagaccg
    18361 ggttcagggt caccctaccg tcgcgatacg ccgtgtcgtc gccaaggagg taggccgagg
    18421 cagcgtggga cgcctcgtgc accgttatac ctactaccag gccgaggagg aaggccacag
    18481 cagcggatgg attctgttgg aagaggctca ggaacataga tgctcgtatg ttagcacttc
    18541 agcaagtcag cacgccgctt cgcggcttgt cagcatttct gcaagaaaca ataggctgac
    18601 tagctgacaa gcgaaagccg caggcttgag cgtgctgaaa tgcgcgagcg gctgcaagac
    18661 gctcgctagc cggactcgga gacggtgacg gtgacgtggt tgccggcatc ggtggtgtag
    18721 gaggagagct gaggatccag gacttcggcc gagtcggcca cggtgcctag ttcggtctta
    18781 ccggccgtaa cgttgtcgtt gacgcccgct tcgccgtcgc tatgcaccag ggcgacgctg
    18841 acgcggacgt ccggattgtc gtctggcttt atctctatga cgttcgcgtt ctggaccata
    18901 ggatcgggac ggatcgctgt gaccgtgccg gttacggggg tgtagacggt ggttcctgta
    18961 gttgcgccga cgtcgacggc accggtctgg ggcccacccc gccccgcggc ctccatgacg
    19021 tagtagtgaa tcccctccgg tgtctctcct ctgaagataa gcccgaggac cacgttggcc
    19081 gataggttct tgccccgcgg ctccatctcg gcgaggctct ccccctcggg gtggtagccg
    19141 atcccggtta ggctcgcagg acgtatcggg gtggagatac tgatccctga ggcctccgcc
    19201 agcaccgtag cgggaccagc gttgttcggg tcgatgggga cggcgcgctg tgccgtgttc
    19261 gaggccctgg cctgcacgaa aaccgcgaag accatcgcga cggcgccgag caccagcagg
    19321 gaaacggcca taatgcgcct tcgcctgtag acttcgcccc gcttcatctt cctggcctcg
    19381 cgcagcccgg tgaagtcgct ctcggtgcga aagcccgctt ggccccgtcg tgttgtcctt
    19441 ctgttggacc gccgatggcc cggcgcttct cgacgatccc caagatgttg ggccctctgt
    19501 gcgtttcccc ctcccatact gggcgaggat tatacagagg ggcgtgcggg gtgtaaactc
    19561 gcgcgtcgcc tcgtccgggg ctggtatatt tcgtatcgta gagatccgat cggagaacgg
    19621 gtgtcagcca gaaaactggt cgtttcgaca tacctgcagt acatcgtgct cgcggtggga
    19681 gtcctactgc tggtgttctt tgtgcgccag gtcagcggcg tgctgctgac cttcctggcc
    19741 gccggtgtcc tagcctacgc cttgaacccc atcatccggc gcctcgaagg attgggggtc
    19801 ccgagggtca tagccgtgat cggggtgttc ctagtcttga tcgtcgttgt cgtcgtgact
    19861 ttgctcttga tcatcatccc agcggtcggg caggtacaga tcgtggtccg taacccgcaa
    19921 gatctcgtcg accagatcac agcccaggtc aataggctcc aggacattcc ctacgtcggc
    19981 cggtacgttg ccgggatgga ccaggaccga atcctgcaac tcttgaggga gaacgcgccg
    20041 tcagcagaac aggtcgcgaa cgttgcgacg ggcgtcatcg gtggggtgtt cggggtcttc
    20101 gggactcttt tcaacttgct gctgttggtg cttgtctccg tctatctgct gctggagcgg
    20161 gagaagatca cccgcgccct gctcggcacg atccccgaga cggtccggga ccagagcctg
    20221 gagctcttct acgaggtcga gcacactctc gtcaggtacc tgagggggca gatcttcctg
    20281 tgcgcgatca tgggtgttat cggagcggcc ataatgtact tcaccgtcgg gaagtacgcc
    20341 ctacccgtgg gggcctgggt ggcggcgacg gagattatcc ccgtcatagg cgccttcctc
    20401 ggggcagtac ccgcggtcgc gatcgcgctc ttcgtacccg gcggcgggtt cctcgatgcc
    20461 ctgctggtcg ccggactctt ccttatagcc cagcagttgg aaggcaacgt gctcgtaccg
    20521 aggatacagg gcggctcggt gggggtccat ccactgtggg tgctcttcgc cacgctggcc
    20581 ggaacggcgc tctacgggtt ggtcggcgcc gtcttcgccg tgcccgtagt cgccatcatc
    20641 gccgcgacgc tccgctacct gcgcgggacg ctgatcttcg aacgctgggg gagaccgccc
    20701 gtcaagcccg ccgaggagga gccggaagcg ggctcgcagg ctgccgggct tgtccccggc
    20761 ccccccggga accagaagga ggaccaatga ctctccgtga accccacaag ccctccttta
    20821 gagaggcttc gaaagacgcg ctgttcgtca tcctgcgtcc gctcgtcagg tggctctcca
    20881 tcatgcgggt tcgccccgac acgctcacgg tcgtcggctg gaccctggcg ctcttttccg
    20941 cggtgctctt cggcctggga tacgccagga tcgccggtgc ggtgatgctc ctgggggggc
    21001 tcttcgacgc gctcgatggg gccgtcgccc gcgagtcgaa aagcatgagc tccttcggcg
    21061 cctttctcga ctcgaccctg gaccggctct cggagtcggc ggtcttcgtc ggcctgatct
    21121 tcttctacgc cagctcggcc cgcccctacg aagctttgct cgccggtctc gcgatgacgt
    21181 tctcgctcct tacctcctac gcccgcgccc gcgccgaagg cctgggcatc gactgcaagg
    21241 tcggactgct ggagcgcgcg gggagggtcg tgatcctctc tgtgttctcg atagccggcc
    21301 tgccgacggt cggccttggc ctactcgcgg caggcgccct cgtaaccaca gcccagcgca
    21361 tcctccacgt ccgccgtgcc gcccgcggat aaggagaccg gccgagcagc cggcgattac
    21421 acccgcaaga ctgcctacga ggccacccgc ctctccaacc gcatgaaaca ggagggacat
    21481 ccagccccgc tcggagatcc cacctcaggg gtcgtgctcg tgctcgaaga gcccttcggt
    21541 ccccgcacca tcgaagccct ggagctcagc ctgcaggccc tcagacttca gcaagcatac
    21601 gtgacctacg agtcgaccgg actgctcgcc cagcagatcc aaaccacaga accggccgtc
    21661 ctcgtggcaa taggcgccgg cgccgcccgc gatatcgacg ccatcgacta cccactcgtc
    21721 agacaaccct tctcggatgc acaacccggt gtgtggttct cctggaccaa gggcacgata
    21781 ggcctcctta ctcccgccct cgctccggcg ctggacgacg acgcagccaa acgacgcttc
    21841 tggctggcct tcctgaccct gagagacctc acataacata ccaccatcgc ccaatcctcc
    21901 aaacatactc tgcaacataa gctctttcgg ccacattaca tatattaaat tttgttaatt
    21961 attcatttcg agtaggaaac actaattccc catacttaag ccattttcat aaacgggggg
    22021 taccctcgtg gaaagtgggg gtgtattgca caacatatag tgttcttcta aagtgcccgc
    22081 cctaggaggg gggattctct cacagcgggt cccctttaac aaactacata atctactgga
    22141 aggagcctgg gcgtatgcct cggagggcga gtttgttcgt gtttctcggt cttgccattg
    22201 cgctcgtgtt cgtaacgagc atctccgctg gggccgagcc ggacggcggc atccgggcga
    22261 agaaggctga cgtcgccgct gcccaggacc gtttgacgga gattcgcatg gaggctggtt
    22321 ctgccgaggc ggcgtacaac aatgcgctct acgagatgaa ccagttgaac ggccagatca
    22381 ccaggacgac ggaagagctc gaggtggcca aggagcgact cgccgtggcc aagaagacta
    22441 tggaacagca ggctacgcag gtttacaaga gtgcagacgt cgctttcatg gatgttctgg
    22501 ttggggtgga cgacttctcc gagttcgcct ccaggctgaa attgtgggtg aggctactcg
    22561 acaaggagag ggccgagttc ctggaggttc gcgaggcccg taacgagctg gccgcgcgca
    22621 gggacaggct cgaggcccag cgtgagcaga gggtcgctgc ggtcgagacg gctatcgccc
    22681 agagagagcg cgccaacgag gccgaggcgc gggcatcggg ttatctggac tccctcgacg
    22741 ccgagctcag gggtgcgatc cgggctgcgc aggatcggca ggccgcgcag gctcgcgcag
    22801 cggcggcggc gatggccgag aaagggcagg agcctgtagg agagcccgag ccgataccgg
    22861 aggtacaggt ctcccaggtc aagcaggccg gggtgccgcg ggccgatctg caatccgaac
    22921 gggctgccgc cgagcgcaag gccgccgcac gggccaccgc cgagagggcc gagcggctcg
    22981 cagaaaagcg cgcggccgag cggaaggccg cccggaaagc ggcagagcag gccgccgccg
    23041 agcgggaggc cgccaggctg gcggccgaga aggttgccag gaaggaagcc agagaggagg
    23101 cccagaggca ggccgagctc gccgcccggc gtgccgcaaa gaggcaggcc gcgagagagg
    23161 aggcagagcg gaaggccgag caggcagccc gtcgagccga gatcgaggag gccgccgcgg
    23221 aacaggccgc cgccgagcgg gaggccgcca ggctggcagc ggagcggcgt gccgatcggc
    23281 gggccgagca gcgggctgaa gaacgcgccg ccgagagggc cgaagccagt gcttccgcga
    23341 gcgccgccgc tgaggaagaa gccgccggtg agcggtgggc cgagaagcgc gccaaagaac
    23401 gcgccgaggc cagcgcatct gccagcgcat ctgtttccag ttcgaaaggc tctgagcgag
    23461 gcggaggtgg tcgggacgaa ggagcttcct ccgccagcgc gacggcgagc gcctccgctt
    23521 ccgggacgcc tgccggtggt ggcggaggta gggtgagtgg tttgggtgaa gacgtgctgg
    23581 ccgtcgccga cgatcacctg gggaccccgt acgagttctc tcctccgggc ccttgcgtgg
    23641 ccttcgaggc cgaggactgc tcctgcttca cgatgctggt cttccaggag ttcggcatcg
    23701 ccctacccga ttcaccaggg gggcagatgg gctacgggac cccggtccac ggtgctcccc
    23761 aggccggaga cctgctcttt tggagcgagg acggcagcgg atacatcacc cacgttggca
    23821 tagccatggg cgacgggact accatccacg cccaagtcca taatgaggtg accatcaccg
    23881 gcataaacca catagccggc tacgtcgggg cgaggaggct tctctaagga atctctctgc
    23941 tagacgggta gcgagggagg tccaacgcgg gcccgtctct ggcgttctcc tggcggtggt
    24001 agaatcagca tgattcgtag caaccggaga ggagcaccct cttatgggct tcgcgttcat
    24061 cgccctgata gtggccatag tggtggtggc gtcggtcttg tacagtgcca tcaaggtcgt
    24121 caaggagtac gagcggggtg tgatcttccg cctcggccgg gtccaggggg gggccgaaag
    24181 gaccaggact cttcctgttg tggccgctcg tggacatgat ggtcaagata gacttgagga
    24241 ccatgaccat ggacgttcct ccccaggacg tcataacccg tgacaacgtc cccgctaggg
    24301 tgaacgccgt agtctacttc cgggtcacgg accccaacag gagcgtaatc gaggtcgaga
    24361 accacgtgct tgcaaccagc caga
//
LOCUS       DATMVF010000042        22198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_646730, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000042 DATMVF010000000
VERSION     DATMVF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22198
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646730"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VN178_01785"
     CDS             complement(<1..376)
                     /locus_tag="VN178_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWS79725.1"
                     /translation="MYATLALLQAVGVGFAAIVVVILAIFVASVVFSAIKVVKEYERG
                     VIFRLGRVQGGPKGPGLFILLPLIDRMVKIDLRTVTMDVPPQDVITRDNVPARVNAVV
                     YFRVTDPNRSVIEVENHVLATSQ"
     gene            complement(390..920)
                     /locus_tag="VN178_01790"
     CDS             complement(390..920)
                     /locus_tag="VN178_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWS79726.1"
                     /translation="MMVIPPVIGVILILVGIVLFIVDLSVTNHGLLTAGGIVTLLAGG
                     MVLLVAGVPYSGVLLGAVVVAAISMGGVLLGVLGSLRASKGRPSLTGKEGMIGEVGTV
                     RRPVGVDSEGWVFVHGELWRAELAFAPKQSNQLESEPMIEVGRKVRVVGFGEGGEVQV
                     VPIELPGRSRDIDRPG"
     gene            1206..1784
                     /locus_tag="VN178_01795"
     CDS             1206..1784
                     /locus_tag="VN178_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HWS79727.1"
                     /translation="MARETDTTRLPDAREDRVIECPVCSSVMTTMEAEGVTVDVCAGG
                     CGGIWFDWFELARVDEVHESAGEKFLEVERDPTLRPDLSKRIRCPRDGEIMMRHFHSV
                     KRGVLVDECPRCAGFWLDAGELAGIRNEFATQEERKQAAQEYFSELFDPDLAVERAKT
                     MEDLRKARRIAYAFRFICPSYYIPGEQDWGAF"
     gene            2035..2736
                     /locus_tag="VN178_01800"
     CDS             2035..2736
                     /locus_tag="VN178_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWS79728.1"
                     /translation="MPMSLEEEVRLLSMVDILGPLSGEELEDLAKRAPDTFLEQDDIL
                     YTPKESTERLFILKKGRVQCYEIDEGGNEITLSVVEDGNVFGEMALTGQSLEGLYVRA
                     ITPSTVVSLRREDVEDLITMKPEVGLRLVRELAERLRDSERRYADVVGKDVPARLATL
                     ILRLVDSEGVVSSESYQIPTHYTHEQLGSMIGCKRVAVTRAFRKLEGGGAVELKDHRI
                     VVKNIDTLRELSEAI"
     gene            3889..4761
                     /locus_tag="VN178_01805"
     CDS             3889..4761
                     /locus_tag="VN178_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWS79729.1"
                     /translation="MGKDRVVDLCLISLLAGGHVLLTDIPGVGKTTLAKAISSSIAAD
                     FSRIQFTPDLLPSDITGTSIYDPGEARFGWIPGPVFAPVLLADEINRATPRTQSALLE
                     AMAEGQVTVEGETRPLPDPFFVVATQNPHQFHGTYPLPEGQLDRFMVATSMGYPGNHA
                     ERGIVRAQVERRPLEEVEPVVEIREILEARRLVKAVQVHDEVLAYAVAIAEATRDHPA
                     VSLGASPRGSIALVRVAQARAATRARDFVGPEDVKALAPEVLAHRLSVRGGTTGTTAE
                     SALKEILDSVVPPV"
     gene            4766..5932
                     /locus_tag="VN178_01810"
     CDS             4766..5932
                     /locus_tag="VN178_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWS79730.1"
                     /translation="MRLRSIVKPALIIFLGLVLYLIASNVGAGWLYVTVATLAGVVLI
                     SLPLPWLGVRHVGITRRAPVVATAGEPFDCSLMLENNGHLPRYMLEVEDRFAGDTGRA
                     VAVRVRRGETETVGYTVENPRRGIYSGGDVVVESRAPFGLFYGRRSTRAASDTVVYPR
                     TFDVAGLPPSAVTDAEWGDRSESSTLHRGHGGEFWGVRDYRPGDPARLVAWKKSARGL
                     PSGKLAVIELAQETHPPFVLAMSLDAGAPTEAREMVVSAGASLLLYALGDGREVWADA
                     GPENTAFPEEPTRDSILTWCAGLRASRPPSKEGASVEIRPSTRRPDTARGSGPSGSPG
                     ATEVRAVVLVSCLEFAGPGPWMSQGEEQEFMQGVEAGGRRVARLGAEVAEPWRI"
     gene            6009..8210
                     /locus_tag="VN178_01815"
     CDS             6009..8210
                     /locus_tag="VN178_01815"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HWS79731.1"
                     /translation="MSFRWPEIKLPGSTKRKPEDSVELRAWVLAAVLVGEAAVLTTGY
                     FGVVTGVLVPFLTVVAFVVSYLRRRERNILIKVMLAFGALAALTMFFREVLSSLYDTR
                     VPLARLFLWVQVIHAFDLPARKDLSYSLVSGLILVAVGAVLSTSLWYGLFVLAFLLCA
                     MGAMTQMNLSEARERAGVQVSRSRGLMVGTVVPSFFAVVAVGLVCFSLLPQRQAMNLT
                     MMPTSAFRNVGENFTGGVQNPYYRPPGGDPFAGPPQAILADSYHGFQPYMDLRSRGRL
                     SDEVVMKVRSEETVPYRGVAFDDYNGKGWEISTGDTAEKLISNNLRFDTITATNTELR
                     EGPVREVAQVFRIEKDSPNIIFGAYRPETVFFPTSTIKIDPYASLRAPYQIPEGSAYS
                     VISSLPNASPAQLRSAGTVYPEEIADRYTQLPPTGLERTHALASRLTEGTTNPYDAVL
                     RMNQYLKETYPYDLSIPPQMEEMDAVEYFLFEQKRGYCEQFSSSLAVMARSLGIPARV
                     ATGYAPGEYNPFTGYYDVRASDAHAWVEVYFPGYGWSTFDPTPSFDSTPWQYRAAGNL
                     QGGKVFGFMAKRLGEAAGPVATAAGTLMRGVARLDPASIIVVGLLVGGAYLVFFYTRK
                     FVAYRSRETLVVRPIKVSDARLYSRYKKITTALQEIGIVREPHETPEQYARRAAHELH
                     EPGLNRLGEIYLYARFRNAVPSTLVEEFDRLEPQALAAIERLKEAHTARSG"
     gene            complement(8490..8999)
                     /locus_tag="VN178_01820"
     CDS             complement(8490..8999)
                     /locus_tag="VN178_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79732.1"
                     /translation="MILMDLNEQVDWDFSRALRKARLRRLVLRLRGKPTTCSIAPSFD
                     EIQGTLRAYNRVRRGIRVVDLRKIVGSVGRSRDFDNSFMPLRAAVGERWKRVDLAFYR
                     DQTLPRVSLYKVGDEYFVEDGNHRISVARYHGLPDVEADVTELWPAHADSARALSGDF
                     SISASQHAA"
     gene            complement(9109..11814)
                     /locus_tag="VN178_01825"
     CDS             complement(9109..11814)
                     /locus_tag="VN178_01825"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS79733.1"
                     /translation="MDRQILRAKLYVPRHRANAVPRSRLHQRLDEGARRDLTVVSAPA
                     GFGKTTLLADWSQRSELPVAWVSLDERDDDPVRFLLYVIAAIETIHEDFGRSTRAFLG
                     SLQSREELEPVLTALSNEILELPGDFVLVLDDYHSVRSEAIHGALAFLLDHWPPPMHL
                     VIAGRTSPPLPLPRLRARGRLTELGVPDLRFSLEEATDFLGRTMGLNLTAKRVAALEE
                     GTEGWIAGLQLAAHALKDREAKFRSMEPIAGGARYVFDYLAEEVLSRQPEDVREFLLK
                     TSIVETLSGPLCEALTDTSDGQEMLEWLEKNNLFLVPLDDEGRYYRYHHLFAAFLRER
                     LGRIHPEEIPELHRRAGLWEEADGCLSGAIEHALVAEDFGRAADLIEEETGVRRTYVD
                     ASLLLRWLGTLPDDLVRPRPQLSLLYAWALAHSGELEDAERRLWDTEEAMKLDAGAST
                     AGLSDEERTMLGEICIIRARMAAMRENAPLTTELSNQALDLLPEDELHLRGDVALDLG
                     HAYCSAGDIESASEAFARAAATGRAADDLRTALFALRYQASLEISRGRLRKAEELLLD
                     GQRLAESRPDGVPSVAGIIHTGMGELLYERGDLDEARRLLEEGIEHGRRSGEAKILVY
                     GYVNLARVLMARGDAGGAHSLIRKAGGLTPRWPLIWAWQARFWLEQGDVESAVRWSRE
                     YEATEDYMNYPRHFERISMARVLLGEGRTDEALDSLGNLLENALSEGRMAHEIELRAL
                     LALTYERLGATNEALEHLERALTLAEPEGFVRVFVDEGLPMAALLERVIRSPQDDGSY
                     HGAPHGTPDIYAGTLLEHFALQAADSGNGRPRSGRAPGLEPLSGREVEVLELVAAGRS
                     NAEIASELYLSVGTVKAHVHHIFGKLLVRNRSQAVARARELRLLG"
     gene            complement(11921..12688)
                     /locus_tag="VN178_01830"
     CDS             complement(11921..12688)
                     /locus_tag="VN178_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79734.1"
                     /translation="MRHLSTRGLSYSLVGAATLVALVFLLPLVDAMPESFLAVRMVAY
                     ALVLVPIALLLHGFFVAAGGNSTSRLLALGAVSGALGLSSAFLASPEDLESSSGIMVL
                     LALSFADLSRILAAACVGVALARYITSVGVVLIIVLAAIASDLFSVFAGPTRALVQED
                     SPVLDALLLVFPTFGSALGFGLGVSDFIFLALFTAASRFLDLRYAATLLGVCFAAFLA
                     VTAGLLLLRPLPALPFIAIAFVLVNADLIVASLAKRR"
     gene            complement(12697..13671)
                     /locus_tag="VN178_01835"
     CDS             complement(12697..13671)
                     /locus_tag="VN178_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWS79735.1"
                     /translation="MLALYWSLLRYRVAVMIILFMLLGAAWHGRLTSIDAPLLLMILA
                     LASSYVSATSVNDIADREIDQINHPGDKGRPLVTGAAGTTDLWVLFGVSSVLAVALAL
                     PAGPGAAGIMLLSVVIGVLYSLPPARLSYRTFLAPLTLAVAYVGIPYWAGVVVVVETL
                     QTSDVLLLTALYLLFAGRIILKDFRDREGDAAFGKPTFLLKYGKKATCLASLCGLCVG
                     DAILVAALADSRWLAALLQLYIASIVTMLYRLYRASSRKDEQLSIGVGAKMGNGLLIT
                     LLGTLILSNQGADWITQLIFGLALTGLYTLNFMDFLRHPERATIGYKG"
     gene            complement(13680..14501)
                     /locus_tag="VN178_01840"
     CDS             complement(13680..14501)
                     /locus_tag="VN178_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79736.1"
                     /translation="MLKRFSTLAVALALFGLAGAAVVVTVPGLFAGPVRPGFTVFLIT
                     GVAAIILTAGLFGYIYRLGMGFGRTVLVLAAGYNALIAAVKLGLAPAALYQANREQAF
                     DAAFGDPNDLWFYLGVGSGVLLLYLLVFGVMYGVFKRRFRRRSLPCELPPEQSGRWSN
                     GAIVVGAVAVVALAASFLWIMPVVYVGFPTFSYLFYIFSTFGAAITLALVLAAVLAYR
                     SFDVVEKRSVRLGDATLLASFFWLGLALILLYHVMWVVFLLTLVSIWPFQTYTPK"
     gene            complement(14657..17482)
                     /locus_tag="VN178_01845"
     CDS             complement(14657..17482)
                     /locus_tag="VN178_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWS79737.1"
                     /translation="MAVLVGCIVLLGWTFAVGSLRIDLPGPVAMNPVSAVGLLLAGVS
                     AWLLQDERAGRLARRISRVCALVVVLFGLSRLIQVLFGLEPGIDQFLFPGRLASEAAS
                     TGFPNRMAFDTALDFVLIGGSLLYLDRRTRRGLWVSQYLALTVFVLSVLAFAGYIYGV
                     ANLYGNTSYIPIASMTLVVLSAGVLCARPERGLMAVVTSGNVGGVVARRLLPAAILVP
                     LVFGWFRLRGQQIGLYGTELGVSLITVANMFVVAALVWWCARLLYRIDGRRKLAEEEM
                     QAARERAEEANRAKSEFLANMSHEIRTPMNGVIGMTDLLLDTRLDEEQREYAETVNTS
                     GQTLLAILNDILDFSKIEAGSLSLETINFELRREVEEVAALLAGHAHEKGLELISYVE
                     PGTPTAVRGDPFRLRQVLTNILGNAIKFTEEGEVTLRAHLVEDTPDTVTVCFEVSDTG
                     IGMTEEQRSRLFQPFTQADASTTRRYGGTGLGLAISKQLVLMMGGAIGVRSEPGVGST
                     FWFTTRLEKRPEGGRTAPAPRSGLRDLRVLIVDDHETNRRILHKQISSWGMEDDMAEN
                     GPEALEALRVAAESGEAYDLAVLDMQMPDMDGIQLARAISADPAISSTRLVLLTSIGI
                     NINETARMAGVEVVLNKPVRQSQLHDALATMLGTPAETKDRPSRGRVGPAPRATPAAE
                     VRATRGHVLLVEDYPVNQVVAIRMLERSGYRVDAVNNGSEAVEAFSKSPYGAVLMDVQ
                     MPEMDGYEATAEIRRREGSGPRTPIIAMTADAMEGDREKALAAGMDDYLAKPVKHEEL
                     DAVLERWINPPVHTTDEDSGTLAETPGLDLTVLKSRRGSQNDGEPDRLARIVGLFIDD
                     VPLRLDELRQAVERGEAQTVDETAHMLKGGSGYMGAARMVKICAGIQVLVASGELSRA
                     PELLDELEAEFERIRPALQSASGLRNG"
     gene            17859..18500
                     /locus_tag="VN178_01850"
     CDS             17859..18500
                     /locus_tag="VN178_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79738.1"
                     /translation="MIGRIRDAFAGNEHPGGRYLQGSFEGCEPYEEVGPFEKVEDWRG
                     IDAGFLDGHANALSFFSEAGFRYFLPAYLISDLRGRLRTADPLFHLTHGFSDWTTEVP
                     AGARTLLIRHGRSAFINPRRYGAMTSYDYARWRLSVFTREEAKAIVAYLEFKRDLDPN
                     VIDKTAIDAALQSFWLERARTAPQARSLEQHIAEQQEYLGAISPQIEEGTGER"
     gene            18554..18886
                     /locus_tag="VN178_01855"
     CDS             18554..18886
                     /locus_tag="VN178_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79739.1"
                     /translation="MRVVPGKQERLAGLFVVVGSAIVFAPAIWLLQRWGEAVAHDPSD
                     VWGARWLGEHLTAHPYLTAGVIFLSVVLSSGVVMLILVSLAKRLAARLTDRGRPPASG
                     SGEKDSGA"
     gene            19120..21990
                     /locus_tag="VN178_01860"
     CDS             19120..21990
                     /locus_tag="VN178_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWS79740.1"
                     /translation="MKRSLDIALRYSVAALAVAVALLAKLLLDPLTVQDTPFLLVFSA
                     IIVSAWYGGLGPGLMATGLSVLATDYFFLYPRGSFSGFSVEGIDVATLALEGVLVSVL
                     TSSLRSARDRARRSTLEARSHQERLRESEERFRLMVEGVKDYAIFMLDPGGCVTTWND
                     GAERIVGYTEEEILGRHFSSFYPDEDVERGHPEDVLRAALAEGRCVEERIQVREDGSR
                     FWASAVITTLRDGKGNLEGFSMVVHDMTERKHTDNVLRFLAESSATLSSSLDYRDTLT
                     NVARLAVPTLADWCAIDVMEEDGTVERLAVEHSDPEKIALAYELQERYPSDPEMTRGV
                     GKVLRTNEPDMMAEIPEELLDQVAMDVEHRTIMRELGLRSYMVVPMVARGRSHGAITL
                     VSAESGRRYEETDLRLAEELARRAALAVDNAKLYEEAQREISERRDAEEALLESEGRY
                     RTVVKQAAEGIFVVDIDTKLILEANVAYRNLLGYTAQDMLGLGLTLYDVVAHDRKSID
                     QYLEQILEKRVHFIGERRHRRKDGSLVDLEVSSSVISYGGGEALCVIVHDITERKRSA
                     ERLQRSLDALLGLYEAGQILSSSLEREEIGSRLLEIASRVSNLTAAVISIPDEEGELR
                     IWRSSGLEGLWRQARFAPDAVAARQRVLETRKYQALRLQQPEDADESQLVSLNLPLLV
                     RGRLIGVLEAYGPEALMERQYEETLISLANQGASALENARLYAELAKRENRLQDLIRK
                     LITAQEEERRKVSYEVHDGLAQTAAGAHQLLQAFARQHPPTSDKGRKDLARVLQLVQQ
                     TVGEARYVIADLRPTALDDFGLAAAVRLQVEKISSQGSQVDYEEALGDERLPPEVETA
                     LFRVTQEALTNVHKHAPSARVRITLRRLNDSVRLQVRDWGPGFDPEEITDGGGPGERL
                     GLSSMRERVALLGGHLKVRSSPGEGTEVVAEIPSDQIEGRGR"
     gene            21998..>22198
                     /locus_tag="VN178_01865"
     CDS             21998..>22198
                     /locus_tag="VN178_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS79741.1"
                     /translation="MNGERAVLPKVARILIADDHALVREGLRTMLSGEDGIEVIAEAR
                     DGKQALDLCRELEPDLVLMDVRM"
BASE COUNT         4217 a         6992 c         7120 g         3869 t
ORIGIN      
        1 tctggctggt tgcaagcacg tggttctcga cctcgattac gctcctgttg gggtccgtga
       61 cccggaagta gactacggcg ttcaccctag cggggacgtt gtcacgggtt atgacgtcct
      121 ggggaggaac gtccatggtc acggtcctca agtctatctt gaccatcctg tcgatcaagg
      181 gaaggaggat gaagagacct ggtcctttcg gccccccctg gacccggccg aggcggaaga
      241 tcacaccccg ctcgtactcc ttgacgacct tgatggcgct gaatacgacc gaggcgacga
      301 atatcgcgag gatcaccacc acgatagccg cgaatcctac cccgactgcc tgcaacaagg
      361 ctaacgttgc gtacacgaga gatcctcctc tatcctggcc ggtcgatatc ccggcttcga
      421 cctggcaatt cgatgggcac tacctgcact tcccctccct caccgaaacc caccaccctc
      481 accttgcgtc ccacctctat catgggctcg ctctctagct ggttggactg tttgggggcg
      541 aacgccagct cggcccgcca cagctcgccg tgcacgaaca cccacccctc agagtccacc
      601 cccacgggcc ttcttacggt gccaacttcg ccgatcatcc cctccttgcc cgtaaggctc
      661 ggcctccctt tggaggcgcg caaagaaccc aggaccccca agagcacacc ccccatgctt
      721 atcgcggcga ccacaacagc accgagcaac acgcccgagt aggggacgcc cgccacaagg
      781 agcaccatgc ctccggcaag gagcgtcacg atgccccccg cggtgagcag accatggttg
      841 gtaacgctca ggtctacgat aaagagtacg atcccaacga ggatcaggat cacccctatc
      901 accgggggta tcaccatcat cggaacagtg ccgcctggtc ggtcgataga tatacgaatg
      961 acggtagcat cgtcgctcga gcataattgt accccacatg acagcacgtt ccggcctttc
     1021 aggcctccgc ttgtcagcat ttcaggtccc gtagtcggag acgagtcgtg atgagtcagt
     1081 gctcctctgg aattctccca agcgcacggc gtctcgccac actacactta gctgaaatgc
     1141 tgaagtgctg acaagcgaca gccgaaggct ggagcgtgct gaaatgctga agggaaagga
     1201 cggagatggc gagagagacg gacaccaccc ggctaccgga tgccagagag gaccgcgtca
     1261 tcgagtgccc ggtgtgctcc agcgtcatga ccacgatgga ggccgagggc gtgacggtgg
     1321 atgtgtgcgc cgggggatgc ggtgggatct ggttcgactg gttcgaactc gcgcgtgtgg
     1381 acgaggtgca tgagtcggcg ggcgagaagt tcctcgaggt ggagcgggat cccaccctcc
     1441 gtcctgacct gagcaagcgc atccgctgtc ccagagacgg tgagatcatg atgcgccact
     1501 tccacagcgt aaaacgcggt gtccttgtag acgagtgccc gaggtgcgcg ggcttctggc
     1561 tggacgccgg cgagctcgcc gggataagga acgagttcgc cacgcaagag gaacgcaagc
     1621 aagccgcgca ggagtacttc tccgagttgt tcgatccgga tctggccgtg gagagggcga
     1681 aaacgatgga ggacctgaga aaggcgcgaa ggatcgccta cgccttccgc ttcatctgcc
     1741 cgagttacta tatccctggt gagcaggact ggggggcgtt ctaaagcgat catctttcag
     1801 ctgatcgcgc atgagagcat gccggtccct cctctgggga gtcgcaccac cgtcgatggc
     1861 gcctttcccg gaggaggctg ccgtattccg agatgcgaaa ggtgttacga tgggagctca
     1921 cataacaaca gcttttgcgg atgcgggccg actccctcct gagttcatta ggggatgtcc
     1981 ttagaagatg gcgatcaagc gcgtttctcg taggagcgaa ggagcgccac gtctatgccg
     2041 atgtcccttg aagaagaagt gcgtctgctt tccatggtcg atatcctggg tcccctctca
     2101 ggtgaggagc tggaggacct ggccaagagg gcaccggaca ccttcctcga gcaagacgac
     2161 attctctaca cccccaagga gagcacggag aggcttttca tcctcaagaa gggcagggta
     2221 cagtgctacg agatagacga gggtggaaac gagatcaccc tctccgtggt cgaggatggc
     2281 aacgtcttcg gcgagatggc cctcacgggc cagagcctcg aaggcctcta cgtcagggcc
     2341 atcacgccgt ccacggtggt ctccttgaga cgcgaggacg tggaggacct gatcacgatg
     2401 aaacccgagg tgggcttgag gttggtacgg gagctcgccg agaggctgcg cgactccgag
     2461 cgccgctacg ccgacgtggt cggcaaggac gtcccggcgc gccttgccac cctgatcctg
     2521 aggctggttg actccgaagg ggttgtgagc agcgaaagct accaaatccc cacccactac
     2581 acccacgagc agttgggcag catgataggc tgcaagcggg tggccgtcac cagggcgttc
     2641 cgcaagctcg agggaggcgg tgcggtcgag ctcaaggatc atcgcatagt cgtcaagaac
     2701 atagataccc taagagagtt atctgaggct atctagaatg ccgttcgtgt tggtggccac
     2761 gttgcttgcg acgttcgccg cagcggtagt tgtggagttc gtcttgtttg gccgatctgt
     2821 gaataggaca agaagatagc cggggcggct acccgccgcc ccggccaact tggtgcgaaa
     2881 cccagtggtt gcgatccctg atatgagggg gatgctctga ttgttgttgt gcgccaccgg
     2941 atagcaccaa acatacgatc cgagcacaac ctttgctgta tccggcgata caccacttcg
     3001 tctcttggta ttacaccgcc tcgcgttgat cctcgtgccg gtatctcgcc tctccccaca
     3061 caaggagagc ggcctcctcc gagagccagt tcaaataatc caggtggcca cagtcggggc
     3121 accgaagcac ccatacccct tgtgcgtcca agatcggctc ccggtcctcc agctcgccac
     3181 tacgtccaca aaaacaactc tctcgcatct ggtgcctcct tccattcgtt ctctttcatc
     3241 gcccgacact tcctcgtctt tgcgacggtc cgcgctctcc ttgcgttgac gggccgtcac
     3301 gtatctcatg cacccagcgt atctttgggg tgttagctgg gcgttaatgg gagcgcctgg
     3361 gacgtaggtg gttccaccta tttttctgaa caacagcggg atgcggaagg gtcaatcctt
     3421 ccgcatcccg cgccgtcagc attcagctgt cagccttcag cgttttcttg ctgaaagctg
     3481 aacgctgaca gctgatagcg cgctgtgcat gaggctcaag gctcatgcac agcgctgtag
     3541 ggttggtgga tcctactccc tccccacgta cgaaccgttc acccggattc cgtcgtccct
     3601 cagaaaatgg ccacctgacc tatacactga gccccgacgg ccgggtatcc tacgtgcgca
     3661 acgcaagcgg acggcgcgtg tgacctacac tctgtcgtac tgaagaaggg tgatggtgat
     3721 gagggtgacg cttctgttgg cttccgtggc ctcagggctg ccggtatgcg gggtagcgca
     3781 gtgacttcgg attctagagg ggtagccata caccgtagtc gggtcgaaag tcaggtagga
     3841 gtcagagaag tctacgagcg gctgcttggc tcggtccgta gggtggtcgt gggcaaggac
     3901 agggtcgttg acttgtgcct gatctcgctc ctcgcaggag gccacgtcct gcttacggac
     3961 atacctgggg tcgggaagac gacgctggcg aaggctatct cgtcctcgat cgcggccgac
     4021 ttctcgcgca tccagttcac cccggacctg ctgccctcgg acatcacggg cacgagcatc
     4081 tacgaccccg gggaggcccg tttcggctgg attccgggcc ccgtcttcgc gccggtcctg
     4141 ctcgccgatg agatcaaccg cgccacccca cgcacgcaga gcgcgctgct ggaggctatg
     4201 gccgagggac aggtgacggt ggaaggcgag acgcggcctc ttcccgatcc tttcttcgtc
     4261 gtcgcgaccc agaacccgca ccagttccac ggtacgtatc cgttgcccga gggacagctc
     4321 gaccgcttta tggtcgcgac gagcatgggc tacccgggga accacgcgga gcgcggcata
     4381 gtgcgcgcgc aggtcgagcg acgtcctcta gaagaggtcg agcccgtcgt ggagataagg
     4441 gagatactgg aggccaggag gctcgtgaag gcggtccagg tccacgacga ggtactcgcc
     4501 tacgccgtcg cgatcgccga ggcgacgcgc gaccacccgg cggtctccct gggggcctcg
     4561 ccgcgcggtt ctatcgccct ggtcagggtg gcccaggcgc gcgccgctac gcgggcgcgg
     4621 gacttcgtgg ggcccgagga cgtcaaagct cttgctcccg aggttctggc gcacaggctc
     4681 tcagtgcgcg gcggtacgac ggggacgacg gcggagagcg cactgaagga gatcctagac
     4741 tccgtagtgc ctccggtgta ggcacatgcg tttgcggagc atcgtcaaac cggccctcat
     4801 catcttcctc ggccttgtgc tgtacttgat cgcctcgaac gtcggcgccg ggtggctcta
     4861 cgtaaccgtg gccacccttg ctggggtagt gctgatctcc cttcccctgc cgtggctcgg
     4921 agtgcggcac gtcggcataa cgaggcgcgc gcccgtcgtg gcgacggccg gcgaaccttt
     4981 cgactgctcc ctgatgctag agaacaatgg ccacctcccc aggtacatgc tcgaggtcga
     5041 ggaccgcttc gccggcgaca cgggaagggc cgttgccgtg cgggtccgcc gcggcgagac
     5101 agagaccgtc gggtacacgg tggagaaccc gcgcaggggt atctactccg gcggagacgt
     5161 cgtcgtcgaa tcgagggcgc cctttgggct cttctacggg cgccgcagta cgcgggccgc
     5221 ctctgatacc gttgtctacc ccaggacctt cgacgtagcc ggactcccac cctcggcggt
     5281 cacggacgcc gagtggggcg acaggagcga gtcatcgacg ctgcacaggg gtcatggggg
     5341 cgaattctgg ggcgtcaggg actaccgtcc aggggacccg gcacgcctgg tcgcctggaa
     5401 gaagagcgcc cgcggcctgc cctccgggaa gctcgccgtc atcgagctgg cccaggagac
     5461 gcacccccct ttcgtgctgg cgatgagcct cgacgccggg gccccgaccg aagccaggga
     5521 gatggtcgtc tcggccggag cttcgctgct cctgtacgcc ctcggcgacg gcagggaggt
     5581 ctgggccgac gcaggaccgg agaacacggc cttccccgag gagcctaccc gcgacagcat
     5641 acttacctgg tgcgcgggcc tgcgggcgtc caggccgccg tccaaggaag gggccagcgt
     5701 cgagatacgc ccttccacaa ggaggccgga caccgccagg ggatcagggc catcaggctc
     5761 gcccggcgcc accgaggtac gggccgtcgt gctcgtgtct tgcctcgagt ttgcggggcc
     5821 tgggccgtgg atgtcgcagg gagaagagca ggagttcatg cagggcgtgg aggccggggg
     5881 acgcagggtg gcgcggctcg gagccgaagt cgccgagccg tggaggatct gaaaatgagg
     5941 cttcaggcat caggtttcag gcttcaggat tcgtgcggaa gcaggcgtga gaaccaggag
     6001 acgctgtgat gtcgttccgt tggccagaga tcaagctccc ggggagcacg aaacgtaagc
     6061 ctgaggactc tgtcgagttg cgggcgtggg tgctcgccgc cgtactggtt ggggaggcag
     6121 ccgtcctcac gaccgggtac ttcggcgttg tgaccggtgt cctcgtaccc ttcctgacgg
     6181 tcgttgcgtt cgtggtcagc tacctgagga ggcgggagag gaacatcctc ataaaggtga
     6241 tgctcgcttt cggcgccctg gcggcgctca cgatgttctt cagggaggtg ttgtcctcgc
     6301 tctacgatac acgggtgccg ctcgccaggc tcttcctctg ggtgcaggtc atacacgcct
     6361 tcgacctgcc cgcccgcaag gacctttcgt actcgctggt gagcggcctg atcctggtcg
     6421 ccgtaggcgc cgttctctcc acgagcctgt ggtacggcct gttcgtgctg gccttcctgc
     6481 tctgtgccat gggggccatg acccagatga acctctccga agcccgcgag cgggccgggg
     6541 tgcaggtatc gagaagcagg ggcctcatgg tcggcaccgt cgtgcccagt ttcttcgccg
     6601 tcgttgcggt cggcctggtt tgcttctctc tcctccccca gcggcaggcg atgaacctca
     6661 cgatgatgcc gacctcggct tttcgaaacg ttggggagaa cttcaccggc ggcgtccaga
     6721 acccttacta caggccgcct ggcggcgacc ccttcgcagg ccccccgcag gccatcctgg
     6781 ctgactcgta ccacggcttc cagccttaca tggacctccg cagccgcggg cggctctccg
     6841 acgaggtcgt gatgaaggtg cgaagcgagg agaccgtgcc ctaccgcggg gtcgccttcg
     6901 acgattacaa cgggaagggg tgggagatct cgaccggtga caccgcagag aagctcatct
     6961 cgaacaacct gcgcttcgac acgatcaccg ccaccaatac cgagttaaga gagggaccgg
     7021 tcagggaagt cgcccaggtt tttcgtatcg agaaggactc cccgaacata atcttcggcg
     7081 cctaccgtcc cgagacggtc ttcttcccga cgagcaccat caagatcgac ccgtacgcct
     7141 cgctcagggc gccttaccag atccccgaag gctcggcgta ctcggtcatc tccagcctcc
     7201 ccaacgcctc ccccgcccag ctcaggtccg cagggacggt ctatccggaa gagatagctg
     7261 acaggtacac acagctccct ccgaccggtc tcgaacgcac ccacgcactc gccagcaggc
     7321 tgaccgaggg tacgacgaac ccttacgacg ccgtgctgag gatgaaccag tacctgaagg
     7381 agacctatcc ctacgacctc tccatcccgc cccagatgga agagatggat gccgtcgagt
     7441 acttcctctt cgagcagaag cgcggctact gcgagcagtt cagctcctcg ctcgccgtga
     7501 tggcccgctc cctagggata ccggccaggg tggcgacggg ctacgccccc ggcgagtaca
     7561 acccgttcac cggctactac gacgtccgcg cctcggacgc ccacgcctgg gtcgaggtct
     7621 acttccccgg atacggctgg tcgaccttcg atcccacccc gagcttcgac tctacgccct
     7681 ggcagtacag ggcggctggc aacctgcagg gcggcaaagt cttcggtttc atggcaaaga
     7741 ggctcggaga ggccgcggga cccgtggcca cggccgcggg aaccctcatg cgcggcgtgg
     7801 cgcgcctcga cccggcgagc ataatcgtcg tagggctgct agtaggaggt gcctacctgg
     7861 tcttcttcta caccaggaag ttcgtcgcct atcgtagccg cgagaccctg gtggtgcgcc
     7921 cgatcaaagt ctccgacgcc cgtctgtaca gcaggtacaa gaagatcacg accgcactcc
     7981 aagagatcgg gatcgtccgc gaaccccacg agaccccgga gcaatacgcc cgcagggcgg
     8041 cgcacgagct gcacgaaccc ggtctgaacc ggctcggaga gatctacctc tacgcacgct
     8101 tccgaaacgc cgtgccaagc accctagtcg aagaattcga ccgcctggaa ccacaggctc
     8161 tcgcggctat cgagcgattg aaagaagcac atacagcacg ctccggctga cgcctccgct
     8221 tgtcagctag tcagcacggc ggcttcgccg ccttgtcagc acgcttcggc ctttaaggcc
     8281 tccgcttgtc agctagtcag catttcaggt ttcgtagtcg gagaggactc tcaaagaata
     8341 cagactcccc tggcagtctc tcaagcgcac tagagtctca ccacacttca cattgctgac
     8401 cggctgacaa gccgcctggc gaagccgggc ggcgagctga caagcgaagc ccgaagggcg
     8461 gagcgtgctg aagtgctgat aagccgcctt caggcggcgt gctgacttgc tgaaatgctg
     8521 aaatcgcccg atagggcgcg tgctgaatcg gcgtgcgcgg gccacaattc ggtcacgtcg
     8581 gcctccacgt ccggcaagcc gtggtagcgg gcgacggaga tgcggtggtt tccgtcctcg
     8641 acgaagtact catcgcccac cttgtagagg cttacccgag gtagcgtctg gtcccggtag
     8701 aacgccagat ccacgcgctt ccaccgctct cccactgcgg cgcgaagggg catgaaggag
     8761 ttatcgaagt ccctgctgcg tcccacgcta ccgacgatct tccgcagatc gacgaccctt
     8821 attcccctgc gaacccggtt gtacgccctg agcgtcccct ggatctcgtc gaaagagggt
     8881 gcgatgctgc aggtggtggg tttccctcgc agacgtaata ccagccgccg cagcctcgcc
     8941 ttacgcagcg cccgactgaa atcccagtct acctgctcgt tcaagtccat caggatcatc
     9001 gctcgactcc tctctgtagg ctacagagcc agtctaggag cagaggtctc tggtttaatc
     9061 taccgaaggt aataccttgt ggtttaccgc tggtttattc tggaagggct aaccgagcag
     9121 gcgcagttcc cgcgccctcg cgacggcctg ggagcggttg cgtacgagga gcttgccaaa
     9181 gatgtggtgg acgtgcgctt tgaccgtgcc cactgagagg taaagctcgc tggcgatctc
     9241 cgcgttcgac cggccggcgg cgacgagttc gagcacctca acctcgcgtc cgctcaaagg
     9301 ttcgagcccg ggggcacgcc cacttcgggg tctcccgttg ccggagtccg ccgcctgcag
     9361 ggcaaagtgt tccagcaggg tgcccgcgta gatgtcgggt gtgccatggg gtgcgccgtg
     9421 gtaggatcca tcgtcctgcg gcgaccggat caccctctct agcaacgccg ccatcggaag
     9481 cccctcgtcg acgaagacgc gcacgaagcc ctccggttcg gccagcgtga gcgctcgttc
     9541 caggtgctcc aacgcctcgt tcgttgcgcc cagccgttcg taggtgagcg cgagcagcgc
     9601 tcggagctct atctcgtgcg ccatccggcc ctcggagagg gcgttctcga gcaggttgcc
     9661 gagggaatcg agggcctcgt cggtcctgcc ctccccgagc agcacgcggg ccatcgagat
     9721 ccgctcgaag tggcgtgggt agttcatgta gtcctcggtc gcctcgtact cccgcgacca
     9781 acgcaccgcc gactccacgt cgccctgctc caaccagaac cgtgcctgcc acgcccatat
     9841 tagcggccac ctcggtgtga gtccgccggc ctttctgatg agggagtgag cccccccggc
     9901 atctccccgg gccatcagca cgcgggccag gttgacgtag ccgtagacca gtatcttcgc
     9961 ctcgccgctc cgcctgccgt gctctatacc ctcctccagg agccttctcg cctcgtcaag
    10021 atccccgcgt tcgtagagca gctcgcccat ccccgtgtgg atgatgccgg cgacagacgg
    10081 cactccatcc ggccggctct cggccagccg ctgtccgtcc aacagcagct cttccgcctt
    10141 ccgtagtcgt ccacgggaga tctccagtga agcctggtac ctgagggcga acagggccgt
    10201 cctgaggtcg tcggccgcac gtccggtggc ggcggccctg gcgaacgctt cgctggcaga
    10261 ctcgatgtct ccggccgagc agtaagcgtg tcccaggtcc agcgcgacgt caccgcgcag
    10321 gtggagctcg tcttcgggca gcaggtctag ggcctggttg gagagttcag tggtaagcgg
    10381 cgcgttctcc cgcatggcag ccatgcgggc gcggatgatg cagatctcgc cgagcatcgt
    10441 gcgctcttca tcggagagtc ccgccgtcga cgctccggcg tcgagcttca tggcctcctc
    10501 cgtatcccaa agccgacgct cggcgtcctc cagctctccg gaatgggcga gcgcccaggc
    10561 gtagagcagg ctgagctgcg ggcgggggcg caccagatcg tcaggcagcg tccccagcca
    10621 gcggagcagc agggacgcgt ccacgtacgt acgccgcacg ccggtctcct cctcgatcag
    10681 gtccgccgcc cgaccgaagt cttctgcgac cagggcgtgc tcgatcgccc cggatagaca
    10741 cccatctgcc tcttcccaga gcccggcgcg gcggtggagt tcggggattt cctctggatg
    10801 tatgcgcccg aggcgttcgc gcaggaaggc ggcgaaaaga tggtggtaac ggtaatagcg
    10861 gccctcatca tccagcggca ccaggaacag gttattcttc tccagccatt cgagcatctc
    10921 ctgcccgtcg ctcgtgtccg tgagcgcctc gcacagggga ccgctcaggg tctcgacgat
    10981 ggaggtcttc agcaagaact cccgaacgtc ctccggttgg cgggagagga cttcctcggc
    11041 caggtaatcg aagacgtagc gtgcgccacc agcgatgggt tccatggagc ggaacttcgc
    11101 ttcgcggtcc ttgagggcgt gcgccgccaa ctgcagaccc gcgatccaac cttccgtccc
    11161 ttcttccagc gccgcgaccc tctttgcggt gaggttcagg cccatcgtac gtcccaggaa
    11221 atccgtcgct tcttcgagtg agaagcgcag gtccggaacg ccaagctcgg tcaaccgtcc
    11281 ccgcgcgcgc agcctcggga gcggcagcgg agggctcgtg cggccggcga tgaccaggtg
    11341 catcggcggt ggccagtggt ccagcaggaa ggcgagggcg ccgtggatgg cctccgagcg
    11401 tacggagtgg tagtcgtcga gcacgaggac gaagtcgccg ggcaactcca ggatctcgtt
    11461 gctgagagcg gtgaggacgg gctccagctc ctcccgagac tgcagcgagc ccaggaaggc
    11521 gcgcgtactc cttccgaagt cctcgtggat ggtctctatg gccgcgatca cgtacaagag
    11581 gaagcgcacg gggtcgtcgt ccctctcatc cagcgagacc caggccaccg gaagctcgct
    11641 tcgctggctc cagtccgcga gcagagtcgt cttgccgaac ccggccggcg ctgagacgac
    11701 ggtcaagtcg cgcctcgcac cctcatccag ccgctggtgc aggcgcgagc ggggtacggc
    11761 attcgcacga tggcggggca cgtagagctt ggccctgaga atctgccggt ccatgagtgc
    11821 cgcttctccc atcgagagaa ttatactcga cgacccgcga attccggact cttgataccc
    11881 ttgacctgat acctgttgcc tgaaacctga tacctggtct tcagcgcctc tttgcgagag
    11941 acgccacgat gagatctgcg ttgaccagca cgaaggcgat ggcgatgaag gggagggctg
    12001 gcagggggcg taggagtagc agtccagcgg tcacagcaag gaaggcggcg aagcagacac
    12061 cgagcagggt cgccgcgtag cggagatcca ggaaacggct cgctgcggtg aaaagggcca
    12121 ggaatatgaa gtcgctcacg ccgaggccga agcccagggc agagccgaac gtcggaaaga
    12181 cgagcagcag ggcgtcgagt acaggagagt cttcctgcac gagagccctg gtcggtcccg
    12241 cgaagacgct gaataggtcc gaggctatcg cggcgaggac gatgatcagg accacgccga
    12301 cggaggttat gtagcgggcg agcgcgacgc cgacgcacgc ggcagcgagt atcctcgaaa
    12361 ggtcggcgaa ggagagcgcg agcaggacca taattcccga actgctctcc agatcctcgg
    12421 ggctggcgag gaaggccgaa gagaggccga gagcaccaga cacagcaccg agagccagaa
    12481 gccgggacgt ggagttgccc cccgcggcga caaagaaccc gtggagcaag agggctatgg
    12541 gaacgaggac caatgcataa gcaaccatcc ggacggcgag aaacgattcc ggcatcgcat
    12601 ctaccagggg caacaagaag accagggcca cgagcgtcgc cgcccccacc agcgaatagg
    12661 agagtccccg tgtagagaga tgtctcaaga cgcgccctat cctttgtacc cgatagtcgc
    12721 ccgctcggga tggcgaagga agtccatgaa gttgagcgtg tagaggcccg tcagcgccag
    12781 gccgaagatg agctgcgtga tccaatctgc accctggttc gataggataa gggtccccag
    12841 caaggttatg aggagtccgt tacccatctt ggccccaacc ccgatggaca actgctcgtc
    12901 cttgcggctg ctcgcccggt agagccggta gagcatagtc acgatgcttg cgatatagag
    12961 ttgcaacagg gccgcaagcc accggctgtc tgccagggct gcgacgagga ttgcgtcccc
    13021 gacgcacagg ccgcagaggc tggccaggca ggtcgccttc ttaccgtact tcaggaggaa
    13081 agtcggcttg ccgaaggcgg cgtcgccctc cctatccctg aagtctttga gtatgatgcg
    13141 gcccgcgaac agcaggtaga gagccgtcaa caagagcacg tctgatgtct ggagggtctc
    13201 cacgactacc accacgccgg cccagtaagg gatgccgacg taggccacgg ccagcgtcag
    13261 cggcgccagg aaagtcctgt aggacagcct ggccggcggc agggaataga gcaccccgat
    13321 cactacggac aacagcatga ttccggcggc gccaggaccg gcgggaagag ccagggccac
    13381 ggcgaggacg ctcgagaccc cgaaaagcac ccacaggtcc gtggtcccgg ccgcgcccgt
    13441 tacgaggggc cttcctttgt cgccggggtg gtttatctgg tcgatctccc tgtctgcgat
    13501 gtcgtttacg ctcgtcgcgc taacgtagct cgacgcgagg gcgaggatca tcagtagcag
    13561 cggcgcgtcg atagatgtaa gcctaccgtg ccacgcggct ccgagcagca tgaagaggat
    13621 gatcatcact gccacgcgat agcgcagcag gctccagtag agggcgagca tcctcatgct
    13681 tacttgggcg tgtaggtctg gaagggccag atgctgacca gcgtcaagag gaagacgacc
    13741 cacatgacgt ggtagagcaa gatcagggcc agacccagcc agaagaaact ggccagtagg
    13801 gtggcgtccc ccagacggac ggaccgcttc tcgaccacgt cgaagctccg ataggccagg
    13861 acggcggcca ggaccagtgc cagcgtaatc gcggcgccga acgtggagaa gatatagaag
    13921 aggtaggaga aggtcggaaa cccgacgtag actacgggca tgatccagag gaagctggcg
    13981 gcgagagcga ctacagctac ggcgccgacg acaatagccc cgttcgacca ccggccgctc
    14041 tgttccggcg gaagctcgca cgggagcgag cgacgcctga agcgacgctt gaagacgccg
    14101 tacatcaccc cgaagacgag caggtagagc aggaggacgc ccgagccgac cccgaggtag
    14161 aaccacaggt cgttcggatc tccgaacgcg gcgtcgaacg cctgctcgcg gttggcctgg
    14221 tacagcgcgg ccggcgcaag gccgagtttc accgccgcta tcagggcgtt gtagccggcg
    14281 gccagcacca ggaccgtcct gccgaagccc atgccaagcc ggtagatgta gccgaacaag
    14341 ccggcggtca agatgatggc cgcgaccccg gtgatcagga agaccgtgaa tccaggccgc
    14401 acgggtccgg caaagagccc cgggaccgtc accaccaccg ctgcaccagc caggccgaaa
    14461 agcgcaagcg ccacggcgag cgtcgagaag cgttttagca tttcagcatt tcagcacgct
    14521 ccagcctcca gctgtcgctc gtcagcattt cagctttcgt gtgcacaagc cctgacttgc
    14581 tgacaaggcg gcaaagccgc cgggctgaag tgctgacaag cgaaggcgct agcctgagcg
    14641 tgctgaaatg ctgacttcaa ccattcctca acccgctcgc ggattgcagg gcggggcgga
    14701 tgcgttcgaa ttccgcttcg agctcgtcta gcagctcggg ggctcgcgac agttcgccgg
    14761 aggcgacgag gacctggatc ccggcgcaga tctttaccat tcgggctgcc cccatgtaac
    14821 cggagccgcc tttgagcatg tgggcagtct catccaccgt ttgggcctct cccctctcga
    14881 ccgcctggcg caactcatcc agccggagcg gcacatcgtc gatgaagagg ccgacgatcc
    14941 tggccagtct gtccggttca ccgtcgttct gggacccgcg ccggctcttc agaacggtga
    15001 ggtcgagtcc gggtgtctct gcaagggttc ccgagtcctc gtcggtcgtg tgtacggggg
    15061 ggttgatcca acgctccagc acggcgtcga gctcctcatg ctttaccggt ttcgcgaggt
    15121 agtcgtccat gcctgcggcc agtgcttttt cccgatcccc ttccatggcg tcggcggtca
    15181 tggcgattat gggggtgcgg ggtcccgagc cttcgcgacg gcggatctcc gccgtggcct
    15241 cgtagccgtc catctcgggc atctgaacgt ccatgagcac agccccgtat gggctcttcg
    15301 agaaggcctc gacggcctcg ctcccgttat tcacggcgtc gacccggtag ccggacctct
    15361 cgagcatcct tatcgccacc acctggttta cggggtagtc ctcgacgagg aggacgtgac
    15421 cgcgggtcgc ccgtacctcg gccgccgggg tcgcgcgtgg agcgggtccc acccttccgc
    15481 gcgacggtct gtcctttgtc tctgcaggag taccgagcat cgtcgcgagg gcgtcgtgga
    15541 gctgcgactg gcgcaccggc ttgttgagga cgacctccac gccggccatg cgggcggtct
    15601 cgttgatgtt gatgccgata gaggtaagca gcacgagtct cgtagaagag attgcgggat
    15661 ccgcgcttat ggcgcgggcg agttggatac cgtccatgtc cggcatctgc atgtccagga
    15721 ccgccaggtc gtaagcctcc ccgctttcgg ccgcgacccg gagtgcctcg agcgcctccg
    15781 gcccgttctc cgccatgtcg tcctccattc cccaggagga gatctgcttg tgcaggatac
    15841 ggcggttcgt ctcgtggtcg tcgacgatga gcacgcgcag gtcgcgcagg cccgagcggg
    15901 gggctggggc cgtccgtcca ccctccggcc tcttctcgag ccgcgtggtg aaccagaacg
    15961 tggagcccac gcccggctcg ctcctcacgc ctatcgctcc ccccatcatc aacaccagtt
    16021 gcttgctgat ggccaggccg agccccgtgc ccccgtagcg gcgggtcgtc gatgcgtcgg
    16081 cctgggtaaa aggttggaag agcctggaac gctgctcttc ggtcatgccg atgcccgtgt
    16141 cactgacctc gaaacagacc gtgaccgtat ccggagtgtc ctcgacgaga tgcgcccgca
    16201 gggtcacctc gccctcctcc gtaaacttga tcgcgttgcc gaggatgttg gtgaggacct
    16261 ggcgcagacg gaaggggtcg ccgcgcacgg cggtcggcgt gccgggctcg acgtagctga
    16321 taagttccag gcccttctcg tgggcgtgtc cggcgagcag ggcggcgacc tcctcgacct
    16381 ccctcctcaa ctcgaagttt atggtctcca ggctcaggct gcccgcctct atcttagaga
    16441 agtccaggat gtcgttgagg atggcgagca gggtctggcc cgaggtgttg accgtctccg
    16501 cgtactcgcg ttgctcctcg tccaggcggg tgtccagaag gaggtccgtc atgcctataa
    16561 cgccgttcat gggggtgcgg atctcgtggc tcatgttcgc caggaactcg ctcttggccc
    16621 tgttggcctc ctcggcccgc tccctcgccg cctgcatctc ctcttcggcc agtttgcgcc
    16681 ggccgtcgat gcggtagagc aaacgggcgc accaccacac cagggcggcg acgacgaaca
    16741 tgttcgccac ggtgatcagg gagaccccga gctccgtgcc gtagagcccg atctgttgtc
    16801 ccctgagcct gaaccagccg aacaccagcg ggaccaggat ggcggccggc aacaaacggc
    16861 gcgccaccac accccccaca ttgccgctcg tgactacggc cataaggccg cgctcggggc
    16921 gcgcgcacag gacgccggcc gagagcacaa caagagtcat cgacgctatg ggtatgtagg
    16981 aggtgttgcc gtacaggttc gcgacgccgt agatgtagcc ggcgaaagcc agtacggata
    17041 acacgaacac ggtcagggcg aggtactgcg agacccagag gccacggcgg gtccgcctgt
    17101 ccaggtacaa cagcgagccc ccgatcagga cgaagtccag cgccgtatcg aaagccatcc
    17161 tgttcgggaa accggtcgag gcggcttctg aagcgagcct cccggggaac aggaactggt
    17221 cgatgccagg ctccaggccg aaaaggacct ggatcagcct ggacaggccg aagaggacga
    17281 ccacgagggc gcatacccgt gagatccgac gcgccagccg ccccgcccgc tcatcttgca
    17341 acaaccaggc cgacacaccg gccaacaaca ggcccaccgc cgagactggg ttcatcgcca
    17401 caggacccgg caggtcgatt ctgaggctcc caacagcgaa cgtccagccc agaagaacga
    17461 tgcagccgac gagtacggcc acgacgcccg aagcccgcga gaacgttctc agggaggtaa
    17521 caagccaggg aatggagtcg ccctccagcc cgggtcgccc cgccgctcca tttccttgcc
    17581 tggctatctc gtactcccgt taaaggctgt tccctacagc aacaggatgc cattgtactt
    17641 gcaagttgcc agcattgcag catttcagca cgctgccttc ggcagcttgt cagcacgctc
    17701 aggcctttaa ggccttcgct tgtcagcacg gcgcttcgca ccttgtcagc atgtcagcaa
    17761 ggggccgcga atgctgacaa gcggaggcga agccggagcg tactgacgag gcggcgcagc
    17821 cgccatgcta acatgctgac agcttgaaca gagaagccgt gatcgggagg attcgagacg
    17881 ctttcgcggg gaacgagcat ccgggaggac gttacctgca gggtagtttc gaggggtgcg
    17941 aaccgtacga ggaggtgggt cccttcgaga aagtggagga ctggaggggt atcgatgcag
    18001 gctttttgga cggccatgcg aacgcgctca gcttcttctc agaggcgggt ttcaggtatt
    18061 tccttccggc gtatttgatc tccgacctgc gcggccggct ccggacggcg gacccactgt
    18121 tccacctgac gcacgggttc tccgactgga ccaccgaggt tcccgcaggg gctcgtacct
    18181 tgctgatcag gcacggcagg tcggcgttca tcaacccgcg gaggtacggc gccatgacct
    18241 cctacgacta cgcgcgatgg cgcctgtccg tcttcaccag ggaagaagcg aaggctatag
    18301 tcgcctacct ggagttcaag cgggatctag accccaacgt catcgacaag acggctatag
    18361 acgcagcact gcagtcgttc tggttggaga gggcgcggac ggcgccccag gccaggagcc
    18421 tcgagcagca catagcggag caacaggagt acctcggcgc gatcagtcca cagatcgagg
    18481 aaggtaccgg tgaacgatga acgttaaccc agcccggatg catcccgcgg agggatagag
    18541 gcggggatta tccatgcgcg tggtaccggg caagcaggaa cgactcgccg ggctcttcgt
    18601 ggtcgtcggt tccgccatcg tgttcgcgcc cgcgatctgg ctcctgcagc gctggggcga
    18661 agctgtagcg cacgatccgt cagacgtctg gggcgcccgg tggctcggcg aacacctgac
    18721 ggctcatccg tacctgacgg caggtgtcat cttcctcagc gtagtgctct cgagcggagt
    18781 ggttatgctg atcttggtca gcctcgcaaa acggctggcg gcccggctta ccgaccgcgg
    18841 gcggccgccg gcgtcgggaa gtggcgagaa ggattccgga gcttgaggaa ctaccgacag
    18901 aatcgccata gggcacggtt ccccacacca gaattctcgg agcgtccacc cgtactcacg
    18961 actgagggtc ctcgcatgat cctgctacgt gccggccgaa atgtgctgcg atgaacactt
    19021 agcgtataat gcgagccacc acttagcacg taagaggtga cgacgatatc ctggaaggcc
    19081 gatagcgctg aggacgccgg ggcagtacca caggcttcta tgaaacgctc tcttgacata
    19141 gctctacgct acagtgtggc ggcactcgcc gttgcggtgg ccctgctggc gaagttactg
    19201 ctagatcctc tgaccgtgca agacacgccg ttcttgctgg tcttcagcgc catcatcgtc
    19261 agcgcgtggt acggcgggtt gggaccgggg ctgatggcca ccggcttgag tgttctggcg
    19321 acagattact tcttcctcta tccgaggggt tcgttctcgg ggttcagcgt ggaggggata
    19381 gacgtggcca cgttggcgct ggagggggtg ctggtcagcg tgcttacgtc gtcgctgcgc
    19441 tcggcgaggg atcgggcccg gcggagcacg ctcgaggcca ggagccatca agagcgtttg
    19501 cgcgagagcg aggagcgctt caggctgatg gtcgaagggg tcaaagacta cgcgatcttc
    19561 atgctggatc cgggaggatg cgtaacgacc tggaacgacg gcgcagagcg catagtgggt
    19621 tacaccgaag aggagatcct gggccggcac ttctcgtctt tctatccgga tgaagatgta
    19681 gagcgtggac accccgaaga tgtactgcgc gcggcgcttg cagagggccg ttgcgtagag
    19741 gagcggatac aggttcgcga ggacggctcc aggttctggg cgagcgccgt catcaccacc
    19801 ctcagggacg ggaaagggaa tctcgaaggc ttctcgatgg tggtgcacga tatgacggag
    19861 cgcaagcaca cagacaacgt gctgcgcttt ctggccgaga gcagcgcgac gctctcttcc
    19921 tccctcgatt acagggacac gctcaccaac gtggcacgcc tcgcggtacc caccctggcc
    19981 gactggtgcg ccatcgacgt catggaggag gacggcaccg tcgagaggct cgccgtggag
    20041 cattccgacc ccgagaaaat cgcgctcgcc tacgagctgc aagagcgcta cccctcagac
    20101 cccgagatga cgcggggtgt gggcaaggtg ctgagaacca acgaaccgga catgatggcg
    20161 gagataccag aggagctttt ggaccaggtc gccatggacg tcgagcacag gacgatcatg
    20221 agggagcttg gcttgaggtc ctacatggtc gtaccaatgg tcgcgagggg gaggagtcac
    20281 ggcgcgatca ctttggtctc cgcggagtcg gggcggcgct acgaggagac ggatctgaga
    20341 ctcgccgagg agctggcgag gcgggcagcc ctggccgtgg ataacgcgaa gctctacgaa
    20401 gaggcgcaga gggagatctc cgagcgcagg gacgcggagg aggctctgct cgagagcgag
    20461 ggacgttaca ggacggtggt caaacaggcc gccgagggga tcttcgttgt cgacatcgac
    20521 accaagctca ttctggaagc caacgtcgct taccgcaacc tgctcggcta tacagcccag
    20581 gatatgctcg ggctcgggct caccctctac gacgtcgtag cgcacgaccg taagagcatc
    20641 gaccagtacc tcgagcagat cctggagaag agggtccact ttatcgggga gcggcgtcac
    20701 cgcaggaaag acggctcgct ggtggacctc gaggtcagct ccagcgtgat ctcttacgga
    20761 ggcggcgagg cattgtgcgt aatcgtccac gatataacgg agcgcaagcg ttccgcagag
    20821 cgactgcagc gcagccttga tgcgcttctg ggcctctacg aggcgggcca gatcctgagc
    20881 tccagcctgg agcgcgaaga gatcggctcc aggctactgg agatagcaag ccgcgtctcg
    20941 aacctcaccg cagcggtcat cagtatcccg gacgaggagg gagagctacg catctggcgt
    21001 tcctcgggtc tggagggatt gtggcgccag gcccgcttcg cccccgatgc ggtggcggcc
    21061 cgccagagag tgctggagac caggaagtac caggccctca ggctgcagca gccggaggac
    21121 gcagacgagt cgcaactcgt gagcctgaac ctcccgctgc tggtgcgggg ccggctcata
    21181 ggtgtgctcg aagcctacgg accagaagcc ctcatggaga ggcagtatga ggagaccttg
    21241 atcagcctcg cgaaccaggg tgccagcgcg ctggagaacg cccggctcta cgccgagttg
    21301 gccaagcgtg agaaccggct gcaggacctg atcaggaagc tcataacggc ccaggaagag
    21361 gagcgccgaa aggtctcata tgaggtgcac gacggcctcg cgcagacggc cgcaggcgcc
    21421 caccagctac tgcaggcgtt cgccagacaa cacccaccca cctctgacaa gggccgcaaa
    21481 gacctcgccc gggtcctgca gctggtgcaa cagacggtcg gtgaggccag gtacgtcata
    21541 gcagatctga ggccgaccgc gctcgacgat ttcggcctgg ctgccgccgt ccgcctgcag
    21601 gtcgagaaga tcagtagcca gggctcccag gtcgactacg aggaagcgct gggagacgag
    21661 cgcctgccgc ccgaggtcga gacggcgctc ttccgggtca cacaggaggc cctcaccaac
    21721 gtccacaagc acgccccctc cgcccgggtg aggatcacac tgcggcgcct gaacgactct
    21781 gttcgcctgc aagtacggga ctggggaccc ggcttcgacc cggaggagat caccgacggt
    21841 ggcggtcccg gcgagcgtct gggcctctcc agtatgcgtg agagggtggc gctgctgggc
    21901 ggccacctga aagtccgcag cagtcccggg gaaggtacgg aagtggtggc cgagataccg
    21961 tcggaccaga tagaaggtag ggggaggtag gagaatcgtg aacggtgaaa gagcggtatt
    22021 gccgaaggta gcacgtatcc tcatagcgga cgatcacgcc ctggtcaggg aaggtctcag
    22081 aaccatgctc tctggagaag atggtatcga agttatcgct gaagccagag acggtaaaca
    22141 agccctagat ctctgcaggg aactcgaacc cgacctcgtc ctgatggacg tgaggatg
//
LOCUS       DATMVF010000043        19988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_417553, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000043 DATMVF010000000
VERSION     DATMVF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19988
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417553"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..840)
                     /locus_tag="VN178_01870"
     CDS             complement(148..840)
                     /locus_tag="VN178_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HWS79742.1"
                     /translation="MPETLHPYLTALADLFYPERCVGCERRASDVLCRTCFDALPRVG
                     SPVCWRCGLPTAFATFVCEECKNVDFGFQSAKAPLKYVGVGKKIVHALKYRGYKRVVG
                     RLAAPLMLQVLDDTRFDAVMPVPLHRSRLRKRGFNQAELLARGVAEKMKATVSDTLQV
                     VRSTKDQVELSAAQRRANVAGAYSATAPLRGKILLVDDVFTTGATMSACAASLVRAGA
                     DEVHALSLCRTC"
     gene            complement(1147..2250)
                     /locus_tag="VN178_01875"
     CDS             complement(1147..2250)
                     /locus_tag="VN178_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWS79743.1"
                     /translation="MQAVVLVGGQGTRLRPVTYDIPKALVPLRNRPFMGYMLDFLRGA
                     GLDGAVLSLGYLPDPIQAYLGDKQDLVGFSVDYAVEDRALGTAGGIKNAEKYLTGDTM
                     VVVNGDVLTGMDLSRAIEVHKASDALATIVLTSVEDPTAYGLVEVDHEMLVHRFIEKP
                     AADEVTTNLVNAGVYVIESEVLAMIPAGREVSIEREIFPELQESGRLRAHITSSYWRD
                     IGTPRSYLAASHDVLSGAVGAHEGFEYLQVDPSVECGKNVRLLPPVSIAEGCKISDLA
                     TIGGRSSLGKGCVVGEGAVVEGSILLDGAEVEAGAVVRGSVIGPRARVGNNSIVRGLS
                     VLGAGCVVGEGNVLDQGIRVNPGVVVSPRSLSF"
     gene            complement(2323..3753)
                     /locus_tag="VN178_01880"
     CDS             complement(2323..3753)
                     /locus_tag="VN178_01880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWS79744.1"
                     /translation="MTGQAFVALLPELISTVFLILVLMAGVFSGNNAGVAAGLASLGT
                     LAVFGSAAVLLATGFSGSYFGGGFVVDGFALYFKLIVAGAAFFAVLAAARWSGQTGDA
                     PEYLTLILSIVLGSTLLVSMRDLFGVFLAIELATIPSYAMVAFDRSRRESAEGGMKYL
                     ITGVIASSVLLYGIVLIYGVSGSAMLQDVANTFSGTLTPVAVIGLVLMISGFAFKLSA
                     APFHFWTPDAYQGAPTSAAAFLSVAPKAATFAILLRILLEGMPDAAPTWTAVMAFLAI
                     ITMFVGNLFALRQRNVRRMLAYSSVAHSGYILAAFAAIQGGGVAFAVQAVLIYSAAYA
                     VMNMGAFLVIDLVGEDTKSYNGLVRTNPGVAASMALFMAALVGIPPLSGFFGKLWIII
                     AGAQSGSVLVYVVVGALVVNTVLSVPYYFGIIRNMIFEEPTTDTARTKGSGAVRFSVY
                     VLAVLTTLFGLLIIPLSTLVGASGLA"
     gene            complement(3753..5207)
                     /locus_tag="VN178_01885"
     CDS             complement(3753..5207)
                     /locus_tag="VN178_01885"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWS79745.1"
                     /translation="MITTITILIPLLGAILLLLLPDEERLVRYAALGVSAVPLVLAVY
                     IYFAFRDDLGVAALTQDVTWIQSLNIGYRVGLDGLGFGMFFLSTLLTVIAVLASWDVQ
                     SNLKQYFAALFVAEVGMLGVFAAQDLILFYVFFEVTLIPMYLLVGVWGDENRRPAAIK
                     FFIYTFLGSTVMLAGFLAFGILADTYSMNALGGGGGLSRTAQIAVAAPILFGLLVKVP
                     AVPLHSWLLDVYVSSPTSTNVLLSGVLPKLGTYGLIAVAIPLLPQGVAPFLPYVAAFG
                     VINIIYGAFVAFLQPDLKALVAYSSIGTLGFILLGAASGNAVGLNGAVFQQVSHGLYS
                     ALLFILVGVIAARTGTRRIEDLGGLAARMPWAGGLLALGALAAMGLPGLAVFISEFMS
                     IMGVYATFPVQGVLAALGIILSAMYLLYMLARVIFGPIERPAYEEITDAGPVEMASIV
                     PLAVLLVVLGVFPALLMSIQRPAVDAVLIVIGGS"
     gene            complement(5204..7186)
                     /gene="nuoL"
                     /locus_tag="VN178_01890"
     CDS             complement(5204..7186)
                     /gene="nuoL"
                     /locus_tag="VN178_01890"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWS79746.1"
                     /translation="MQTVGQQLIITAIPTLPALVFLVLVFTGRLLKENAQYVAILGVT
                     ISLVFSVFALLFVAGIVPGGGAPIEFSVNWIDLGLGGSFPMGVYIDGLAAVMLVVVSF
                     VSLMIQLYSGAFMEGDKRFAWFYAVLNLFTASMLGLVLAPNFIQLYVFWELVGLCSYL
                     LVGHWFEKPSARDAAVKAFVVTRVGDAALFVGIIIFWATTGSTAFTDISAAAQAGFIG
                     GSLFTTAVILVFIGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVART
                     YDIFVQSPTAMLVVAYIGGFTALMAATMALVKKDIKRVIAYSTVSQLGYMMLGLGLGS
                     YTAGIFHLYNHAFFKALLFLCAGSIIYAMDSYNLFEMGGLRSRMPITFWTMLIAGLSL
                     SGIFPFAGFWSKDAIVASAYDEHYYLLFGMALLTVFLTAFYIFRAIFLAFTGEPRTEP
                     AREATESPGIMTGPMMLLAFFAIVSGWVGIPEGFGLPVRDYFSEFVHPSEFAAATLDH
                     AAHAFSFVLGGISVVVGLLGIGLAYTLYVARPELAGQLAARFSWLYTFLDKGWYFDAL
                     YGATFVRSAMWLGRATRGFDRNVIGGFVGGVGRGTLGTGGLLQRLQNGGVQSYALFIL
                     LSVLIIGVIVGAQYAAIVVGVIALATIAAFAVGSRL"
     gene            complement(7197..7556)
                     /gene="nuoK"
                     /locus_tag="VN178_01895"
     CDS             complement(7197..7556)
                     /gene="nuoK"
                     /locus_tag="VN178_01895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWS79747.1"
                     /translation="MPQIPTQVQEFLDTLVAAPPLEGYLVVGALLFAIGAWGALIRRN
                     AVVVFMCVELMINGVNLTLVAFADYLPNAGGLGVSYAVLVIAIAAAEIAVGLAIVLAV
                     FRSRRTVNVDEVTSMRG"
     gene            complement(7556..8092)
                     /locus_tag="VN178_01900"
     CDS             complement(7556..8092)
                     /locus_tag="VN178_01900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWS79748.1"
                     /translation="MTVAFAFLAGMTLVSALGVVLSRSVVHSALFMSGSFLGVSGYFV
                     MLNAPALAAFQVLIYVGAVTVVILFGIMFTQKPQVRRYRTIINRQFWAGLMVAAGVGA
                     FLAYVLSAEDWGGAEAGNGPREVGAFGRDLLGVSQGSEIFTLLFEVASVLLLVAVVAA
                     IVVSRRKIGEGVDRGVRE"
     gene            complement(8089..8601)
                     /locus_tag="VN178_01905"
     CDS             complement(8089..8601)
                     /locus_tag="VN178_01905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWS79749.1"
                     /translation="MPQLGQGIMKGMGVTLQHLFGQKITRQYPEYKRELPERSRGMLT
                     VDMDRCIACLQCMRICPDHCISIEQVKRDADGSGKAKPYAVGFVIDDSRCMYCSLCVE
                     VCPVNCIYHTEEFEAQAYNRLELARQFGERPVDPTVDPKPAAKKKKKPTAKKAPKTVE
                     RPVTEKDDAA"
     gene            complement(8611..9612)
                     /gene="nuoH"
                     /locus_tag="VN178_01910"
     CDS             complement(8611..9612)
                     /gene="nuoH"
                     /locus_tag="VN178_01910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWS79750.1"
                     /translation="MQTLLNLLEVEPIRLLISFGAVLFLVLNLAAVLTMAERKVSGYI
                     HLRYGPNRVGPRGLLQPAADVFKLFTKENVSPGRSDLWVFLGAPIAMFIPAALVWLVI
                     PFAPNAVVADLNVAILFFVAITSIGALGVVMAGYGSRSAFSLLGALRGAAQMISYEVP
                     LILSLLGVVMLAGSLSLTDVVRAQGDTIWTWYFLPQLPMFLTFYISGIAEVKRVPFDL
                     PEGESEIVGGFMIEYSGMTWALIQAAEFASMAVMSAITVTLFLGGWQPPLPFLDLGHF
                     NWVWFGIKTALIMFTFQWIRWSVPRLRMDQLMDLGWKVLVPMTLVWLFVTAGAMLVI"
     gene            complement(9613..10800)
                     /locus_tag="VN178_01915"
     CDS             complement(9613..10800)
                     /locus_tag="VN178_01915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWS79751.1"
                     /translation="MLGEERRAEHQGALTEPASGRLASPDIRDEFGLETIDMNMGPQH
                     PAMHGLLRLILELDGETVVRCDPVMGYLHRCQEKIAENRMYPAVIPMTDRLDYFANMH
                     NEHGYCLAVEDLLDVEIPPRAEYIRVLMCELMRLASHIPSIGFLMLELGAFTPILYAF
                     RERERIQSIFEAVTGARMMFHYIRIGGVKADLPPEIPQKIWEYIESLEAHFQRDFVDL
                     IEGNEIFISRTRNVGRMTQEQAVEMGVTGPALRCTGVDFDIRMDYPYSVYPDLQFEVI
                     TDTAGDVEASYRVRIAECLESIKIIKQCLERMPGGEVMGDTGRRIRPAEGEGFGRVES
                     PRGEFAVHIVSDGSDTPYRVHYRDPSFCNMQLLQEIVPGHYLPDIMPIMGLVDPVSGG
                     WDK"
     gene            complement(10806..11297)
                     /locus_tag="VN178_01920"
     CDS             complement(10806..11297)
                     /locus_tag="VN178_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWS79752.1"
                     /translation="MALTLPEGDLQSRLQRYLDGFGDRHGLEEAEIVGDVVRIVARKE
                     DVPAVLATARETLSIPHLSFITVVDRQGSFELTYELMDFRGATVRVKTAIEGDEPVIG
                     TVTGVYPTANWHEREAYDMFGVAFAGHPDLRRTYMWQDHFDHHPLLKSFEISTKRTFE
                     GLR"
     gene            complement(11298..12047)
                     /gene="nuoB"
                     /locus_tag="VN178_01925"
     CDS             complement(11298..12047)
                     /gene="nuoB"
                     /locus_tag="VN178_01925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HWS79753.1"
                     /translation="MQQFNEPNLVTTSTDKLFNWARKNSLWPLQFGLACCAIEMMSTG
                     MAHNDLDRFGAGFFAASPRQADVMIVSGTVSTKMAERMTRLYEQMPEPKWVIAMGACA
                     ISGGPFLDGYSVLMGADRVIPVDVYVPGCPPRPEALIFGIMTLQKKIERDQQVGYERP
                     RPALVDEDGTISEYLPEREYTEISAGKAPRLEGIRPLKRTYVFERAGMPPASQTDVGW
                     VPPEVKKRLKEEARARKAAEAQDSADGKENA"
     gene            complement(12047..12397)
                     /locus_tag="VN178_01930"
     CDS             complement(12047..12397)
                     /locus_tag="VN178_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWS79754.1"
                     /translation="MVYLYVGIFMLLVTGFVATVLVLARLISPGRRSSAKSQNYETGE
                     MTVTDPWRPFPVRYYVFALLFLVFDVEAAFLFPWAVIYRSLGVYGFVEMVIFVSILAV
                     GLVYAWKKGALKWV"
     gene            12790..13053
                     /locus_tag="VN178_01935"
     CDS             12790..13053
                     /locus_tag="VN178_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWS79755.1"
                     /translation="MAYVITELCIGTKNTACVGACPVDCIYDADDQFVINPAECIDCS
                     MCMPECPVEAIYPGDEVPEEMEHSKTRAAEFDYSSIAPGVEVN"
     gene            13061..14227
                     /locus_tag="VN178_01940"
     CDS             13061..14227
                     /locus_tag="VN178_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HWS79756.1"
                     /translation="MAEMKRRSLEEVKALSVEEAVEIMRQAGIVGAGGGGFPTYFKYK
                     VPQPHLLVNATESEPGYWGDKLVHKENLEEFLQLFEAMKAIFDFEQISLCVHEKDREW
                     FADYAEHAADGFFDVRYVPDTYALGEEKTLVKHATDKRVPRFLDTPQGMRRPGMPPDV
                     GKVVNNSETLLNVYNALFLGKPTTTTVFTVYGEGIDLKVYEVPIGASFAEVLEIAGLD
                     IQNSGHLSLLDGGPYLHDMGIKEIGNGDGYVRRMTNALFLIPKDRQGKEYADQKTDPP
                     EEGIVSLVGKVSGVNIPLGGGLLKPATRLVAEGDEVEYEQKLGEPVDEGFSIGVWASM
                     GGKISSLENNIIAIAEGAVPQEEAEAEAEASGAGGAPPRGEGEPFQEAEAGTKR"
     gene            complement(14423..15493)
                     /locus_tag="VN178_01945"
     CDS             complement(14423..15493)
                     /locus_tag="VN178_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWS79757.1"
                     /translation="MRYRSYTPGAAQSAMDFINGWFGARGLETTRYTGAGPRGDLTSL
                     VVRVGTGDPRILLHGHVDVVPGEDTQFEPREEGDELYGRGVYDMKGALAAMMYAVEDL
                     RKLGCESTVELLVVPDEEGEHGAKTGAEVLISNGHVGDFLITGEPTDFHIGTQAKGVL
                     DVRVTLHGKSAHGSQPWLGKNAALLAYEHYRRVARLPFTEQRSEFFPYPSVNLARIIG
                     GDVLNRVPDRCTYDLDVRFLPNQDPDEIVRQIRSVDLPADVEVLFYREPTYVTRQNPY
                     VKALREVSARHFRGNPVGVGRHGASDVVYFQRAGVPGVEFGPVGGGHHGPNEYVVTSS
                     LETYRQMIVQFVQIVGRNGKVP"
     gene            complement(15661..15852)
                     /locus_tag="VN178_01950"
     CDS             complement(15661..15852)
                     /locus_tag="VN178_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79758.1"
                     /translation="MSTASGSGEDRKSYGYGSGLYFRHSSHRVTEVVRPLEGMEITGA
                     GPGTSSSNSTVPFALLGED"
     gene            complement(15962..19285)
                     /locus_tag="VN178_01955"
     CDS             complement(15962..19285)
                     /locus_tag="VN178_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWS79759.1"
                     /translation="MDIGCCGSSLLALGWAASIAQENTEPGVIEGGATEDTLQTRTLG
                     DAEIAAGEVIVRYTAVSDPADRADARDAVDVEEQDPLLLPRMEVLDLGPGQSVEGAIE
                     ELEANPAVKAAEPNYVMELHATPNDSFFGFQWGLHNTGQTVNNTAGTADADIDAPEAW
                     ERSTGSEDVTVAVIDSGLNLEASDLSPNLWTNPGESGGGKETNGVDDDGNGLIDDVNG
                     WDFVENDNDPDDENNGHGSHVGGTIGARGNDGAGVAGVNWETSLVPMRASPDYSRSLP
                     FSATLDAFVYAGRIGADVVNGSFGGVRGPSQIERDALASAPETLFVFSAGNDGIDTDT
                     NEVHPCNHATSLENVVCVAATDQNDGLASFSNFGYQHVDLGAPGVNIASTFVTPSLTN
                     LNALFAAPGLQIPYSQTFDSDLGGWEFAGVGNTWARTNEAPLQNFGLSDSPGGNYADN
                     VDSTATSPLINLSDRANCALIFDAQTALAPGDSISLEASGDGGTSWRQLVSFGNNPFA
                     TYGETLGSVVDNQSDALLRLRLQTDSATTANGVHFDFPLVGCRPESAPNHVFSNGTSM
                     AAPHVAGAAALLKSLAGQAPATALEDRLLATADPLTALDGKTTTGGRLNLEHATKPPE
                     TEITSPPPQDGKPSQTFTFQTDEENRDNGTTFECRADSEPFGPCSGEGADTRTYSIGE
                     HTFEVRATWYGNADPTPASVTFGVDNQPPTAEADSYTATEDTPLTVSAPGVLDNDTDP
                     NSGDALSVAAPRPISGPTNGSLTFDSDGSFTYKANDGMGDSNTATVSITVSPVNDVPV
                     ATDAARTMNQDGAPITIDFASLVSDGETSDADLTYNIVSGPTPAQGTLSGTGSTRTFD
                     SADNFNGSVEIIYTVTDRGDPDGCGTPDDGCDAPESSDQGKVTVTVSPANNAPTIAVA
                     AGGSCGSASGTMNLTLGDAETAAGSLTLAKSSSKTTLVPNSNITFGGSGANRTVTITP
                     AANKSGNATITLTVSDGQGGTATTTIKVIVGTGNTETINGTSGADMIFGKSGSDTINA
                     NSGRDLACGGDGNDRLRGENGNDTLYGGQGNDCLEGGDGNDKLNGSMGADFFSGGPGT
                     DTATDFTPSQGDTKDATIP"
     gene            complement(19756..>19988)
                     /locus_tag="VN178_01960"
     CDS             complement(19756..>19988)
                     /locus_tag="VN178_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWS79760.1"
                     /translation="GGHRALIRGPRRLQGTIVQAPDLRGGAALVIAGLVSEGTTVVDG
                     ATHILRGYEDFEAKLTSLGATVAFEAGAPVPR"
BASE COUNT         3702 a         6242 c         6551 g         3493 t
ORIGIN      
        1 tttcagcttt gccgctcggt tcgtgaccac ttagcgtggg gttacagcct ctgtgcacgg
       61 actcacttcg gcagggtgtt ccgcgcctca agcgtagggt tgcgtaatgc acgttcttgc
      121 tgactgctga tggctgaaag ctgacagcta acatgtcctg cacaaactca gggcgtgtac
      181 ctcgtccgcc ccggcacgga cgaggctcgc ggcgcacgcg ctcattgttg ctcccgtggt
      241 aaagacgtcg tcgacgagta ggatcttgcc gcgcagaggt gcggtggccg agtaggcgcc
      301 ggcgacgttg gccctcctct gggccgccga gagttcgacc tggtccttgg tgctgcgcac
      361 gacttgtagt gtatccgata cggttgcttt catcttctct gcgacgcccc gggcgaggag
      421 ttcggcctga ttgaagcctc tcttcctcag ccttgaacgg tgcagcggca cgggcatgac
      481 ggcgtcgaag cgggtgtcgt cgaggacctg cagcatcagt ggcgcggcga gccgcccgac
      541 cacccgtttg tagccgcgat acttgagggc gtggactatc ttcttgccga cgccgacgta
      601 cttcaggggc gctttcgcgc tctggaaacc gaagtcgacg ttcttgcact cctcgcatac
      661 gaacgtcgcg aaggcggtag gtagcccgca acgccagcac acggggctgc ccactcgggg
      721 gagcgcgtcg aagcaagtgc ggcagagcac gtcgctcgcc cgccgctcac agccgacgca
      781 gcgctcaggg tagaacaggt cagccagcgc agtgaggtag ggatgtaaag tttcaggcat
      841 caggcttcag cagtggttct ggagctcgcg actgccgcag cgtgttcgcg gctctggccg
      901 gccgcaacgc tttctaccta gagtcggacg gggtgaccgt cagctgtaac ccaatacctg
      961 taacctgaag cccgctgagc gaagcgaagc caaaagctca agtcaccttt agttggcctg
     1021 catttggcct gaggaggtac tttggccatc caacggctcc ggccgcaggt atgtgagata
     1081 tggactgtac cccgggatct cctccacgga atgctggttt tgctacctga ttgtcggtag
     1141 ggaaggctag aagctcaagc tgcggggcga gacgacgacg ccggggttca cgcggatgcc
     1201 ctggtcgagg acgtttccct caccgaccac gcaaccggcg ccgagcacgg agaggccgcg
     1261 cacgatggag ttgttgccga ccctagcgcg cgggcctatg acagaacccc gcacgacggc
     1321 gccggcctcg acttcggcgc cgtcgaggag gatgcttccc tcgaccaccg ccccctcccc
     1381 caccacgcag ccctttccga gcgaggaacg ccccccgatg gtggcgaggt cggagatctt
     1441 gcacccctcg gctatcgaga ccgggggcag gagcctgacg ttcttgccgc actcgaccga
     1501 ggggtcgacc tggaggtact cgaagccttc gtgggcgccg acggcgccgg agaggacatc
     1561 gtgggaggcc gccaggtagc tgcgcggcgt ccctatatcc cgccagtaag agctcgtgat
     1621 gtgggccctg agcctgccgc tctcctggag ctcggggaag atctcacgct ctatagagac
     1681 ctcgcggccg gccgggatca tggcgaggac ctcggactct atcacgtaga cgccggcgtt
     1741 gaccaggttc gtcgtcacct cgtcggcggc cggcttctcg atgaagcggt gcacgagcat
     1801 ctcgtggtcg acctcgacga ggccgtaggc cgtgggatcc tctacgctcg tcagaactat
     1861 ggtggccagg gcgtccgagg ctttgtgaac ctctatcgcc ctggagaggt ccatccccgt
     1921 gagcacgtcg ccgttgacga cgaccatcgt gtcgcctgtg aggtactttt cggcgttctt
     1981 gatcccgccg gcagttccta gggcgcggtc ttcgacggcg taatccacgg agaaacctac
     2041 gagatcctgc ttgtcgccaa gataggcctg tatcgggtcc ggcaggtaac ccaacgagag
     2101 cacagcgcca tcgaggcccg cgccgcgcag aaagtccagc atgtacccca taaagggacg
     2161 gttgcggagc ggcaccagcg ccttcggaat gtcgtaggtt acaggccgca gcctggtccc
     2221 ctgtccgcca acgagtacga ctgcttgcat cggcctagct tacaccaggc ccggatctta
     2281 gaccaatacg gatcgagcga tatttcaacg gcgctctgtt cctcacgcca ggcccgaggc
     2341 tccgaccagc gtggagaggg ggatgatgag aaggccgaaa agggtagtaa ggacggccag
     2401 cacgtagacg gagaatctaa cggcgccgct tcccttcgtt ctcgccgtgt ctgtcgtcgg
     2461 ctcctcgaag atcatgttcc taatgatgcc gaagtagtag gggacggaga ggacggtgtt
     2521 gactacgagc gcaccgacga cgacgtagac gaggaccgag cccgactggg cgccagcgat
     2581 gatgatccag agcttcccga agaaccccga gagtggtggg ataccgacca gcgcggccat
     2641 gaaaagggcc atgctcgccg ccacgccggg gttcgtcctt acgagcccgt tgtagctctt
     2701 ggtatcctcg cccaccaggt ctattacgag gaaggcgccc atgttcataa cggcgtaggc
     2761 ggcggagtag atcaggacgg cctgcacggc gaaggctacc ccgccaccct ggatagccgc
     2821 gaaggcggcg aggatgtacc cggagtgggc caccgagctg taggcgagca tccgcctgac
     2881 gttgcgctgc cgtagggcga agaggttgcc gacgaacatg gtgatgatcg ccaggaaggc
     2941 catcaccgcc gtccaggtcg gcgccgcgtc aggcatgcct tcgaggagga tccgaaggag
     3001 aatcgcgaag gtggcggcct tgggggctac cgagaggaag gcggccgcgc tcgtcggggc
     3061 gccctggtag gcgtcggggg tccagaaatg gaagggcgcc gccgagagct tgaaggcgaa
     3121 gcccgagatc atgagcacga gcccgatgac ggccaccggg gtgagggtgc cggagaaagt
     3181 gttagccacg tcctggagca tcgcggagcc cgagacgccg tagatcaaga cgatgccgta
     3241 gagcagcacg gaggaggcga tgacgccggt gatcaggtac ttcatcccgc cctccgcgct
     3301 ctcgcggcgg ctacggtcga aggcgaccat ggcgtaggaa ggtattgtgg ccagctctat
     3361 ggcgaggaag acgccgaaga ggtcccgcat ggagacgagg agcgtcgaac cgaggacgat
     3421 ggagaggatc agcgtcaggt actctggcgc gtcgccggtc tggccggacc agcgggccgc
     3481 cgcgaggacg gcgaagaagg ccgctcctgc gactatcagc ttgaagtaaa gcgcgaagcc
     3541 gtcgaccacg aagccgccgc cgaagtagct tcccgagaag cctgtcgcca gcaagacggc
     3601 ggcgctcccg aagacggcga gcgttccgag ggaggcgagg cctgccgcca cccctgcgtt
     3661 gttaccgctg aacaccccgg ccatcaaaac caggatcaag aagaccgtgg agatcagctc
     3721 gggcaagagc gcgacgaatg cctgaccggt cactagctac cccctatcac gatcaggacg
     3781 gcgtccacgg ccgggcgctg gatgctcatc agcagggccg ggaagacacc gagcaccacg
     3841 agcaacacgg ccagcggcac gatggaggcc atctcgaccg gtcctgcgtc cgtgatctcc
     3901 tcgtaagccg gacgttcgat ggggccgaag atcacacgcg ccagcatata gagcaggtac
     3961 atggccgaga gtatgatccc cagcgccgcg agcactccct gcacggggaa cgtcgcgtac
     4021 acgcccatga tgctcatgaa ctcggagatg aagacggcga gcccgggcag ccccattgcg
     4081 gcgagggcgc cgagggccag aagccctccc gcccacggca tcctggccgc cagcccgccg
     4141 agatcttcga tgcggcgcgt ccccgtgcgg gccgcaatca cgccgacgag gatgaagagg
     4201 agggctgagt agagcccgtg cgagacttgc tggaagacgg ccccgttcag gccgaccgcg
     4261 ttccccgagg cggcaccgag caggatgaaa cccagcgtac ctatggagga gtaggcgacg
     4321 agcgccttga ggtcgggctg caagaaggcc acgaaagccc cgtagatgat gttgatcacc
     4381 ccgaaagcgg cgacgtaagg gaggaagggt gcgacgccct gggggagcag ggggatggcc
     4441 accgcgatca ggccgtaggt acccagtttg ggcagaactc ccgagagcag cacgttggtc
     4501 gaagtcgggc tggagacgta tacgtccaga agccagctgt gcagcggcac ggcgggcacc
     4561 ttgacgagca acccgaagag gatgggggct gccacggcta tctgggccgt ccgcgagagg
     4621 ccgccaccgc cgcccagggc gttcatcgag taggtatcgg ccaggatccc gaaagccagg
     4681 aatccggcca gcatcaccgt cgagccgagg aacgtgtaga tgaagaactt gatcgcggcc
     4741 ggacgcctgt tctcgtcccc ccacacgccg acgagcaagt acatggggat cagcgtgacc
     4801 tcgaagaaga cgtagaacag gatcaaatcc tgcgccgcga agacccccag catccccacc
     4861 tcggcgacga agagggcggc gaagtactgc ttgaggttgc tttgcacgtc ccaggaggcg
     4921 agaaccgcta tgaccgtgag caacgtcgag aggaagaaca taccgaaccc gagaccgtcg
     4981 agtccgaccc ggtagcctat gttcagggac tggatccagg ttacgtcctg ggtgagcgcg
     5041 gcgacgccga ggtcgtcccg gaaggcgaag tagatgtaga ccgcgagcac gagaggcaca
     5101 gccgaaaccc cgagggccgc gtagcgcacc agccgctcct cgtcgggcaa gagcagcaag
     5161 agtatggcgc cgagtagcgg gatgaggatc gtgatagtcg ttatcacagt cggctcccca
     5221 cggcgaaagc ggcgatcgtt gccagcgcga tcacgccgac gactatagcc gcgtactgcg
     5281 cccctactat gaccccgatg atcaggacgc tcagcaggat aaagagcgcg taactctgga
     5341 ccccgccatt ctgcagccgc tgcagcagcc cgcccgtccc cagggtgccc cgtcccaccc
     5401 cgcccacgaa tccgccgatg acgttacggt cgaagccccg ggtagccctg ccgagccaca
     5461 tcgcggacct cacgaacgtc gcaccgtaga gggcgtcgaa gtaccacccc ttgtccagga
     5521 acgtgtagag ccaggagaat ctggccgcaa gctgccccgc cagctccggc ctcgcgacgt
     5581 agagcgtgta ggcgagcccg atcccgagca gcccgacgac cacagagatg ccgcccagga
     5641 cgaagctgaa ggcgtgcgct gcgtggtcga gcgtcgccgc tgcgaactcg ctcggatgca
     5701 cgaactcgct gaaatagtcc ctcacgggga ggccgaaccc ttccggtatc ccgacccagc
     5761 cgctcacgat ggcgaagaag gccaggagca tcatcggccc cgtcatgatc cccggcgact
     5821 ctgtggcctc gcgggccggc tcggtgcgcg gctcgccggt gaaggcgagg aagatggcgc
     5881 ggaagatgta gaacgcggtg aggaacacgg tcagcagcgc catgccgaag agcaggtagt
     5941 agtgctcatc gtaggccgag gccacgatcg cgtctttgga ccagaagccg gcgaagggaa
     6001 agatccccga gagcgagagc ccggctatga gcatggtcca gaacgtgatg ggcatcctac
     6061 tcctcagtcc ccccatctcg aagaggttgt aggagtccat cgcgtagatg atgctgccgg
     6121 cgcacaagaa caggagggcc ttgaagaagg cgtggttgta gaggtggaag atgcccgccg
     6181 tgtaggaacc gagcccgagg ccaagcatca tgtagccgag ctgcgagacg gtcgagtagg
     6241 cgatcacacg ctttatgtcc ttcttgacca gcgccatcgt cgcggccatc agtgccgtaa
     6301 agccgccgat gtacgccacg acgagcatgg ctgtggggct ctgcacgaag atgtcgtagg
     6361 tgcgggccac gaggtagacg cccgcagcga ccatggtggc ggcgtggatc agggcgctca
     6421 ccggagtagg accctccatg gcgtcgggga gccacacgtg cagcgggaac tgggcgctct
     6481 tgccgacggc gccgatgaag acgagtatca cggccgtggt gaacagggag ccgccgataa
     6541 acccggcctg ggctgccgcc gagatgtccg tgaaggccgt agagccggtc gtcgcccaga
     6601 agatgatgat cccgacgaac agcgcggcgt cgcctacacg ggtcaccacg aaagccttta
     6661 ccgcggcgtc gcgggcagag ggcttttcga accagtgtcc gacgagcagg taggagcaca
     6721 ggccgacgag ttcccagaag acgtagagct ggatgaagtt cggcgccagc acgagcccga
     6781 gcatagaggc cgtgaacagg ttgagcacgg cgtagaacca ggcgaagcgt ttgtcgccct
     6841 ccatgaaagc gcccgagtag agctggatca tgaggctcac gaagctcacc acgacgagca
     6901 tcaccgcggc gagcccgtcg atgtacacgc ccatcgggaa cgagcctccc aggccgagat
     6961 ctatccagtt caccgagaac tctatgggcg ccccgccgcc ggggacgatg ccggcgacga
     7021 acaacagggc gaagaccgag aagacgagcg agatggtcac cccgagaatg gcgacgtact
     7081 gggcgttctc cttgaggagg cgtcccgtga agaccagcac gaggaagacc agcgcgggga
     7141 gcgtcggtat ggccgtgatg atcagctgtt ggcctacggt ctgcacggtt tcccttctag
     7201 cccctcatgg aggttacttc atccacgttc acggtgcgcc gcgagcggaa cacggcgagc
     7261 acgatagcga gccctacggc tatctcggcc gcggctatgg cgatcacgag cacggcgtag
     7321 ctgacgccga gtcctcccgc gttcgggagg tagtcggcga aggcgacgag cgtgagattg
     7381 acgccgttta tcatgagctc gacgcacatg aaaacgacga cggcgttgcg gcggatcaag
     7441 gccccccacg cccctatggc gaaaaggagg gcgcccacca cgagatatcc ttcgagcgga
     7501 ggggccgcga ccagcgtgtc caggaactct tgtacctgtg tcgggatctg gggcactact
     7561 ctcgcacgcc cctatctacg ccttctccga tcttgcggcg gctcaccacg atggcggcca
     7621 cgacggccac gaggagcagc accgaggcga cttcgaacag cagggtgaag atctcggacc
     7681 cctggctgac gccgagcaga tcccggccga aggccccgac ctcccgcggt ccgttaccgg
     7741 cctcggcgcc tccccagtcc tcggcggaga gcacgtaggc gaggaaggcc ccgacgcccg
     7801 ctgccaccat gagaccggcc cagaactgcc ggttgatgat ggtgcggtaa cgcctgacct
     7861 gcggtttctg ggtgaacatg attccgaaca ggatcaccac ggtaaccgcc cccacgtaga
     7921 tgaggacctg gaaagcggcg agcgccggcg cgttgagcat cacgaagtat ccggagacgc
     7981 cgaggaacga ccccgacatg aacagcgccg agtggacgac gctgcggctc aggactacgc
     8041 cgagcgccga gacgagcgtc atcccggcca ggaaagcgaa cgctaccgtc aagccgcgtc
     8101 gtctttctcg gtgacggggc gctctacggt cttcggcgct ttcttggcgg taggcttctt
     8161 cttctttttg gcggctggct tgggatctac cgtcgggtcg acgggacgct cgccgaactg
     8221 cctggccagc tcgaggcggt tgtaggcctg cgcctcgaat tcttctgtgt ggtagatgca
     8281 gttgaccggg catacctcga cgcacagcga gcagtacatg cagcgcgagt cgtcgatcac
     8341 gaatcccacg gcgtacggct tggccttgcc tgagccgtcg gcgtcgcgct ttacctgctc
     8401 gatggagatg cagtggtccg ggcagatcct catgcactgc aggcacgcga tgcagcggtc
     8461 catgtcgacc gtgagcatcc cgcggctccg ttcggggagc tcgcgcttgt attcggggta
     8521 ctggcgcgtg atcttctgcc cgaagagatg ctgcagggtg acgcccatcc ccttcattat
     8581 gccctgtcct aattgcggca tcgaaacctc ctagataaca agcatagctc cggccgtaac
     8641 gaacagccaa acgagggtca tcgggaccag caccttccag cccaggtcca tgagctggtc
     8701 catcctgagg cgcggtacgc tccaccggat ccactggaag gtaaacatga tgagcgccgt
     8761 cttgataccg aaccagaccc agttgaaatg cccgaggtcg aggaagggca aaggcggctg
     8821 ccagcccccc aagaacagcg ttacggtgat ggccgacatg accgccatcg aggcgaactc
     8881 ggccgcctgt atgagggccc aggtcatccc cgagtactcg atcatgaaac cgccaacgat
     8941 ctcgctctcg ccctccggga ggtcgaaggg tacccgcttc acctccgcga tgcccgagat
     9001 gtagaacgtc aaaaacatcg ggagctgggg caagaagtac caggtccaga tggtgtcccc
     9061 ctgcgccctt accacgtcgg ttagagagag gcttccggcg agcatcacga cgccgagcag
     9121 gctcaggatc aggggcacct cgtaggagat catctgcgcc gctccgcgca aagctccgag
     9181 cagcgagaac gccgagcggc tcccgtatcc tgccataacg acgcccagag cgccgatcga
     9241 ggtgatcgcg acgaagaaca gtatggcgac gttgaggtcg gcgacgaccg cgttcggcgc
     9301 gaacgggatg acgagccaga cgagcgcggc cgggatgaac atggctatgg gcgcacccaa
     9361 aaagacccac aggtccgagc gtccgggcga gacgttctct ttggtaaaga gcttgaagac
     9421 gtcggcggcc ggctgcaaca gcccgcgggg tcctaccctg ttcggcccgt accggaggtg
     9481 gatgtagccc gagaccttcc tctcggccat cgtaaggacg gccgcgaggt tgagcacgag
     9541 gaaaagcacg gccccgaagg agatgagcag ccttatcggc tcgacctcga gcaggttcag
     9601 cagcgtctgc atctacttgt cccaccctcc cgagacgggg tcgacgaggc ccataatggg
     9661 cattatgtcc ggcagataat ggccaggcac gatctcctgc aaaagctgca tgttgcagaa
     9721 gctggggtcg cggtagtgga cccggtaagg ggtgtcggag ccgtccgaga ctatgtggac
     9781 cgcgaactca ccgcgggggc tctcgaccct tccgaagccc tccccttcgg ccggacggat
     9841 gcggcggccc gtatccccca ttacctcgcc cccgggcatc cgttccaggc actgcttgat
     9901 gattttgatg ctctccaggc actcggcgat acggaccctg taggaggctt cgacgtcgcc
     9961 cgccgtatcc gtgatgacct cgaactgcaa gtcggggtag acgctgtacg gatagtccat
    10021 ccttatgtcg aagtccacgc cggtgcagcg cagggcgggg cccgtaacgc ccatctcgac
    10081 cgcctgctcc tgcgtcatcc tccccacgtt tcgcgtgcgg gagatgaaga tctcattgcc
    10141 ctcgatcagg tcgacgaagt cccgctggaa gtgggcctcg aggctctcga tgtactccca
    10201 gatcttctgc ggtatctctg gtggtaggtc ggccttgacg cccccgatgc ggatgtagtg
    10261 gaacatcatc cgcgcgccgg tgacggcctc gaagatgctc tgtatgcgct ccctctcgcg
    10321 gaatgcgtag aggatcgggg tgaaagcccc gagctcgagc atgaggaatc ctatggatgg
    10381 gatgtggctg gcgagcctca tgagctcaca catgaggacg cggatgtatt cggccctcgg
    10441 tggtatctcc acgtcgagca ggtcctcgac ggcgaggcag tagccgtgtt cgttgtgcat
    10501 gttcgcgaag taatcgagcc tgtcggtcat cgggatcacc gcgggataca tgcggttctc
    10561 ggctatcttc tcctggcaac gatgcaaata gcccatcaca ggatcgcacc tgaccaccgt
    10621 ctccccgtcg agctcaagga tgagcctgag gagcccgtgc atggccgggt gctggggacc
    10681 catgttcatg tctatggtct cgagcccgaa ctcgtcccta atgtcggggc ttgcgagccg
    10741 gccgctggcc ggctcggtga gggctccctg gtgttctgcg cggcgttctt ctccgagcat
    10801 attctttacc tcaatccctc gaaggtgcgc ttggtgctta tctcgaagct cttgagcagc
    10861 ggatggtggt cgaagtggtc ctgccacatg tacgtcctgc gcaggtcggg atgcccggcg
    10921 aatgcgaccc cgaacatgtc gtaggcctcc cgctcgtgcc agttggccgt cgggtagacg
    10981 ccggtgacgg tccctatcac gggctcgtct ccctcgatgg ccgtcttgac cctgaccgtg
    11041 gccccgcgga agtccatgag ctcgtaggtg agctcgaagg agccctgcct gtcgacgacg
    11101 gtgataaaag agaggtgggg gatgctcagg gtctcgcgag ccgtcgccag cacggcggga
    11161 acgtcttcct tacgggcgac gatccgcacc acatcaccga cgatctccgc ttcctcgagg
    11221 ccgtggcggt cgccgaagcc atcgaggtag cgctgcagcc tgctctggag gtctccttcg
    11281 ggcaacgtga gggccaccta ggcgttctcc ttgccatcgg cgctatcctg cgcctctgcc
    11341 gccttacggg ccctggcctc ttccttgagc ctcttcttca cctcgggcgg gacccagccg
    11401 acgtccgtct gggaggcggg cggcatgccg gcacgctcga atacgtaggt gcgcttgagc
    11461 ggtcttatcc cttccaggcg gggcgccttg cccgcgctga tctccgtgta ctcgcgctcg
    11521 ggcaggtact cgctgatggt gccgtcctcg tcgacgaggg cgggccgggg ccgctcgtaa
    11581 cccacctgct ggtcgcgctc tatcttcttc tggagcgtca tgatcccgaa gataagcgcc
    11641 tcgggtcgcg gcgggcaacc cggcacgtag acatcgaccg gtatcaccct atccgcgccc
    11701 atcagcacgg agtacccatc caaaaacggg ccgccggaga tcgcgcaggc ccccatcgct
    11761 atgacccatt tcggctcggg catctgctcg tagaggcggg tcatgcgctc ggccatcttg
    11821 gtcgagaccg tgccggagac gatcatgacg tcggcctgcc gcggcgaggc cgcgaagaat
    11881 ccggcgccga agcgatcgag gtcgttgtgg gccatgcccg tcgacatcat ctcgatggcg
    11941 cagcaggcca acccgaactg cagcggccag agcgagttct tgcgcgccca gttgaagagt
    12001 ttgtccgtgc tcgtggttac gaggttcggc tcgttgaact gctgcatcac acccacttga
    12061 gggctccctt cttccaggcg tagaccaacc ccacagccag gatcgagaca aagataacca
    12121 tctctacgaa cccgtagacg ccgaggctcc tgtagatgac ggcccacggg aacaggaagg
    12181 ccgcctccac gtcgaagacc agaaagagca aggcgaagac gtagtagcgc acggggaaag
    12241 gtcgccacgg gtcggtaacc gtcatctctc ccgtctcgta attctgggac ttcgcgctcg
    12301 acctccggcc aggagagata aggcgcgcga gtaccagcac ggtcgcgacg aaaccagtga
    12361 caagcaacat gaagatgccc acgtacagat aaaccatccc gtaggaggct ccctgttgca
    12421 aaagacctaa cgccaaatcg gacctttcta cccgataact gcctgaaata tactcgcgcc
    12481 tggtggaaag ttcaagcggt aactaaaagt agcaaaccgc gaacaacagc acgccggctg
    12541 cgccggcttg tcagcatttc agcacgccgc ctctggcggc tctcagcact taagccgtgc
    12601 tgcggccatc gctcgcgcga cgaagccagg ctcccggacg gacgaggctg acaagctgaa
    12661 gtactagcga agcccgaagg gttgagcgtg ctgataagcc gcgaagcggc gtgctgacat
    12721 tgttgacaac gtttgcgcat tggatagact gctttccaga gaatccggca ctagcaaagg
    12781 ggaaacaacg tggcctatgt gatcactgaa ttgtgtatcg gcacgaagaa cacggcttgt
    12841 gtgggtgcgt gcccggtgga ctgcatctac gacgccgatg accagttcgt gatcaacccc
    12901 gccgagtgca tcgattgctc gatgtgcatg ccggaatgcc cggtggaggc catctatccg
    12961 ggcgacgagg tccccgaaga gatggagcat tccaagacga gggccgccga gttcgactac
    13021 tcgagcatcg cgcccggcgt cgaagtcaac tagccggatc atggccgaga tgaagcgacg
    13081 cagcctggaa gaagtcaagg ccttgagcgt ggaggaggcc gtggagatca tgcgccaggc
    13141 cggtatcgtc ggcgccgggg gcggcggttt ccccacctac ttcaagtaca aggtccccca
    13201 gcctcacctg ctcgtcaacg ccaccgagag cgagccgggt tactgggggg acaagctggt
    13261 gcacaaggag aacctggagg agttcctgca gctcttcgag gccatgaagg cgatcttcga
    13321 cttcgagcag atctctctgt gcgtccacga gaaagatagg gaatggttcg ccgactacgc
    13381 ggagcacgcc gccgacggct tcttcgatgt gcgctacgtg cccgacacgt acgccctcgg
    13441 cgaggagaag accctcgtca agcatgctac tgacaagagg gtgcccaggt tcctggacac
    13501 cccccaaggc atgaggcgcc ctggcatgcc gccggacgtc ggtaaggtcg tcaacaactc
    13561 cgagaccttg ctcaacgtct acaacgcgct cttcctcggc aaaccgacca ccacgacggt
    13621 ctttaccgtc tacggcgagg ggatagacct gaaggtctac gaggtgccga taggggcatc
    13681 cttcgcggag gtcctggaga tagccggcct ggacatccag aactccgggc acctctcgct
    13741 tctggacggc gggccctacc tgcacgacat ggggatcaag gagataggga acggagacgg
    13801 ctacgtacgg cggatgacga acgctttgtt cttgatcccc aaagacaggc agggcaaaga
    13861 gtacgccgat cagaagaccg atcctcccga ggagggtatc gtctccctgg tcggaaaggt
    13921 ctccggcgtg aacattcccc tcggcggcgg tctgttgaag cccgctaccc ggctcgtggc
    13981 cgagggcgac gaagtcgagt acgagcagaa attgggcgag cccgtggacg agggattctc
    14041 catcggggtc tgggcgagta tggggggcaa gatctcctcg ctagagaaca acatcatcgc
    14101 catcgccgaa ggcgcggtac cccaggagga ggccgaagcc gaggccgaag cgagcggcgc
    14161 aggcggcgcg ccacctcgcg gggaaggcga gcctttccag gaagccgagg ccggaacgaa
    14221 gaggtagtag cggttatgga aggttccctt ccataaccgc gctggtcagc acgctcaggc
    14281 tttcgccttc gcttgtcagc acggcggctg cgccgccttg tcagcttgtc agccagggct
    14341 tggacacccg aaagctgaaa tgctgaagtg ctgacgagcg acagccgaag gctggagcgt
    14401 gctgaaatgc ttgcaatgag cgtcagggta ccttgccgtt gcggccgacg atctgtacga
    14461 actgcacgat catctgcctg taggtttcca gagaggaggt caccacgtac tcgttcgggc
    14521 catggtgtcc accaccgacc ggaccgaact cgacaccggg tactcctgcg cgctggaagt
    14581 agaccacgtc gctcgcgccg tggcggccca cgccgacagg gttgccgcgg aagtggcgtg
    14641 ccgagacctc gcggagcgcc ttcacgtaag ggttctggcg agtcacgtag gtaggttcgc
    14701 ggtagaacag gacctcgaca tcggcgggaa ggtcgaccga gcgtatctgg cgcacgatct
    14761 catcagggtc ctggttaggt aggaagcgga cgtcgaggtc gtaggtgcag cggtccggta
    14821 cccggttgag aacgtcccca ccgatgatgc gcgccaggtt cacgctcggg taagggaaga
    14881 actcgctcct ctgttccgtg aagggcaggc gtgcgaccct gcggtaatgc tcgtatgcga
    14941 gaagcgcggc gttcttgccg agccagggtt gggagccgtg ggcgctctta ccatgcagcg
    15001 tcacacggac gtcgaggaca cccttcgcct gggtaccgat atggaagtcc gtgggctcgc
    15061 ccgtgatcag gaagtccccc acgtggccgt tggagatcaa gacctcagca cccgtttttg
    15121 cgccgtgctc accctcttca tcggggacga cgaggagctc gaccgtgctt tcgcacccca
    15181 gcttccgcaa gtcctcgacg gcgtacatca tggcggcgag ggcgcctttc atgtcgtaga
    15241 cgccccggcc gtagagctcg tccccctcct cgcgcggttc gaactgcgta tcctcgccgg
    15301 gcaccacgtc gacgtggccg tgcaagagta tccttggatc tccggtcccg acccgcacca
    15361 cgagcgaggt aaggtctccg cggggtcccg cgcccgtgta gcgcgtcgtc tctagtcccc
    15421 gcgccccgaa ccagccattg ataaagtcca tcgcggactg cgccgcgccc ggcgtgtaag
    15481 acctgtagcg cacgagctct cgggtgagcg catggacctc ggtgttctcc cttactaccg
    15541 ccagatgatc ctccgcgctt cggatgccaa caagagcagt ctatatttca gcacgccgcc
    15601 ttgcaggcgg cttgtcagga cggcgctgcg tgcccctcag cacgcttcgg cttcgcccgt
    15661 tcagtcctcg cccagcaacg cgaaaggaac agtcgagttc gaagatgagg tacctggacc
    15721 ggcgccggtg atctccatac cctccagcgg acggactacc tccgtcacgc ggtggctgct
    15781 atgcctgaag tacaggccac ttccgtaccc gtaggatttc cgatcctcgc ccgacccgga
    15841 ggccgtgctc aaactctcac tactcggcag agaaaccaca gagcccaggg cgatggaact
    15901 tgccctgggc tcccttaata tggccggctc ttatcgtgtc gctggttctt ccagtctctc
    15961 tctagggtat ggttgcgtcc tttgtgtcac cctggctcgg cgtgaagtcg gttgcggtgt
    16021 cggtgccggg gccgccgctg aagaagtcag cgcccatgct accattcagc ttgtcgttgc
    16081 cgtcgcctcc ttctagacaa tcgttgcctt gcccgccgta gagggtgtcg ttgccgttct
    16141 cgcccctgag cctgtcgttg ccgtctccgc cgcaggccag gtccctgcca ctgttggcgt
    16201 tgatggtgtc gctaccgctc ttgccgaaga tcatgtccgc gccgctagta ccgttgatcg
    16261 tctcagtatt gccggtgccg acgatgacct tgatggtggt cgttgccgtg cctccctgcc
    16321 cgtcgctcac ggtgagggtg atcgtggcgt taccgctctt gttggccgcc ggggtgatcg
    16381 tcaccgtgcg gttggctccg ctgccgccga aggtgatgtt ggagttgggc acgagagtcg
    16441 tcttcgacga gctcttggcc agcgtcaggc tgcccgccgc cgtctctgca tcgccgagcg
    16501 tgaggttcat cgtaccgctc gcgctgccgc acgagccgcc cgccgcgacc gcgatggtcg
    16561 gggcgttgtt ggccgggctg acggtgacag tcaccttacc ttgatcgctg ctctccggcg
    16621 cgtcgcagcc atcatccggt gttccgcagc cgtccgggtc gccccggtcg gtgacggtgt
    16681 agatgatctc cacgcttcca ttgaagttat ccgcggagtc gaaggtgcgc gtcgatcccg
    16741 tgcccgagag cgtgccctgg gccggcgtcg gtccggagac tatgttgtag gtgaggtcgg
    16801 catcggaggt ctctccatcg gagaccaggc tagcaaagtc tatggtgatg ggggcgccgt
    16861 cctggttcat ggtcctggcg gcatcggtag ccaccgggac gtcgttgacc gggctgacgg
    16921 tgatggaaac cgtagcggtg ttggagtccc ccatgccgtc attggccttg taggtgaagg
    16981 agccgtcgga gtcgaaggtg agcgagccgt tggtcgggcc gctgatgggg cgtggggcgg
    17041 ccacgctgag cgcgtcgcca gagttggggt cggtgtcgtt gtcgagaacg ccgggcgcgg
    17101 agacggtgag cggggtgtcc tcggtggccg tgtagctatc ggcctccgcc gtgggcggct
    17161 ggttgtcgac cccgaaggtg acgctcgccg gggtcgggtc ggcgttgccg taccacgtgg
    17221 cgcgcacctc gaaggtgtgc tcgccgatgg aataggtgcg cgtatcggcg ccctctcccg
    17281 agcaggggcc gaacggctcg gagtccgccc ggcactcgaa ggtcgtcccg ttgtcccggt
    17341 tctcctcgtc ggtctggaag gtgaacgtct ggctcggctt cccgtcctgc gggggcgggg
    17401 aggtgatctc ggtctcgggc ggcttagtgg catgctcgag gttgagccgc ccgccagtag
    17461 tcgtcttgcc gtccagcgca gtgagcgggt ccgccgtggc tagcagccgg tcttcgagcg
    17521 cggtcgcggg cgcctgcccg gcgagcgact tgaggagggc ggcggcgcct gcgacgtggg
    17581 gcgcggccat cgaggtgccg ttggagaaca cgtggttcgg cgccgactcc gggcggcacc
    17641 cgaccaaggg gaagtcgaaa tggacgccgt tggcggtggt ggcggagtcg gtctgaaggc
    17701 gcaagcgcag gagcgcgtcg gactggttgt ccactacgct tccgagcgtc tccccgtaag
    17761 tagcaaaggg gttgttcccg aaggacacaa gctgccgcca actcgttcct ccatcgccgg
    17821 aggcttcgag gctgatgctg tctcctggcg cgagggccgt ttgcgcgtcg aagataaggg
    17881 cacagttcgc ccggtccgag agatttatca gcgggctcgt ggcggtgctg tcgacgttgt
    17941 cggcgtagtt gccgccgggc gagtcggaga ggccgaagtt ctgaagcggc gcctcgttcg
    18001 ttcgcgccca cgtgttgcca actcccgcga actcccagcc gccgaggtcg gagtcgaagg
    18061 tctgcgaata gggaatctga agaccggggg ccgcgaataa cgcgttaagg tttgtcaggc
    18121 tcggggtgac gaaagtgctg gcgatgttga cccccggcgc tcccaggtcc acgtgctggt
    18181 acccgaagtt ggagaaagag gcgagcccat cgttctggtc cgtcgccgcg acgcagacca
    18241 cgttctcgag ggaagtcgcg tggttgcagg gatggacctc gttcgtatcg gtgtcgatgc
    18301 catcgttgcc ggcggagaag acgaacaggg tctcgggggc cgacgcgagc gcatcgcgct
    18361 cgatctgcga cggccctctc acacccccga agctgccgtt taccacgtca gccccgatcc
    18421 ttcccgcgta gacgaacgcg tcgagcgtcg ccgaaaacgg gaggcttctc gagtagtctg
    18481 gggaggcacg catcgggacg aggctcgtct cccagttgac gcccgccacg cccgctccgt
    18541 cgttcccccg cgcgccgatg gtcccgccca cgtgcgagcc gtggccgttg ttctcgtcgt
    18601 cggggtcgtt gtcgttctcc acgaagtccc agccgttcac gtcgtctatg agaccgttgc
    18661 catcgtcgtc cacgccgttg gtctccttgc cgccgcccga ctcgccgggg ttcgtccaca
    18721 ggttggggct caggtcggag gcttccaggt tcaggcccga gtcgatgacc gcgacggtga
    18781 cgtcttcgga gcccgtggag cgctcccacg cctccggcgc gtctatgtcg gcgtcggcgg
    18841 tgccggccgt gttgttgacg gtctgcccgg tgttgtggag tccccattgg aagccgaaga
    18901 aggagtcgtt cggggtcgca tgcaactcca tcacgtagtt cggctcggcc gcctttacgg
    18961 ccgggttcgc ctccagctcc tcgatggcgc cctccacgct ctgtccgggg cccagatcca
    19021 ggacctccat gcggggcagg agcaaggggt cctgctcctc tacgtccact gcatcgcggg
    19081 cgtcggcccg gtccgcggga tcgctcaccg ccgtgtagcg cacgatcacc tccccggcgg
    19141 cgatctcggc atcgccgagc gttcgcgtct gcagggtgtc ctcggtggcg ccaccctcga
    19201 tgaccccggg ctccgtgttc tcctgggcga tcgacgctgc ccacccgaga gcaagaaggc
    19261 tactcccaca gcagccaata tccaaaacaa ccggtgcgtt attggtgtgc gtgtcatcat
    19321 ctcgctagca ctcttttccc cgcgatccct cgtcgaagtg tcgcttgccc tcttcgtagc
    19381 agtgtgcgcg cggcgaggaa agcgcatcac tcaattgcag tattttttgc aatttgtttt
    19441 acgattcata ccaagtccgg gtgaatgcca acggtacagc agggtcagcc actgtccacc
    19501 gcctacctcc tactcctcgc gatggattcc cgctccccgc tttcgcgggg acaagcttcg
    19561 cgagaatgac gcatggggga gagcctcggc gtgcccacag cacgaacgcg gtctccttca
    19621 agcggccccg gtatcagggg aggggcttcc ggctacgctg tggcgccatt ttcggggatg
    19681 tatgctcgta ttgtggacga ccggaggcgg tgagggtgtt tccggctgct ccgggcagac
    19741 cgcaagaacg ctgatctagc gggggaccgg agcccccgcc tcgaaggcga cggtcgcgcc
    19801 tagtgatgtg agcttggctt cgaagtcctc gtagccacga aggatgtgcg tggcgccgtc
    19861 cacgacggtg gtgccttcgg agaccaggcc tgcgatgacg agtgccgcgc ctccgcgcag
    19921 gtctggtgcc tgcacgatgg ttccctgcag gcggcgcggg ccgcggatga gggccctgtg
    19981 gccgccga
//
LOCUS       DATMVF010000044        16060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_49772, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000044 DATMVF010000000
VERSION     DATMVF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16060
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49772"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VN178_01965"
     CDS             <1..427
                     /locus_tag="VN178_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79761.1"
                     /translation="ADFVVERVRDEAGLAAWIEALGSGFGAGPVEAEWAGEMYRRLGL
                     GAEGPWRHYVGLLAGEPVATSTSFFGAGVAGIYFVCTVERARRRGIGAGITLAPLHEA
                     RDLGHTVGVLGSSEMGYPVYRRLGFEEYCRIGLYEWRSG"
     gene            501..1022
                     /locus_tag="VN178_01970"
     CDS             501..1022
                     /locus_tag="VN178_01970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWS79762.1"
                     /translation="MASKSYDRAPEMALEPGYEYYANFVTEKGPVKVRLFAEEAPETV
                     NNFVFLARDGYFDGTTFHRVIADFMVQGGDPTGTGTGGPGYRIPDEFHPGLRHDKPGV
                     LSMANAGPNTGGSQFFITHVATPWLDDRHAVFGEVVDGMEVVGAIRERDPQRDPEPGD
                     RIERIEIEELPRT"
     gene            1109..1492
                     /locus_tag="VN178_01975"
     CDS             1109..1492
                     /locus_tag="VN178_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS79763.1"
                     /translation="MSKEVADRFVEALRKLEEDRDVDALVEIHAQDCDVGNVAVPKTF
                     SGHDGLREFWTGYRDTFGEMKSEFQTVFADDAGHAALEWNTSGKANGNDVSYDGVSLL
                     EIEEGKVTRFRAYFDPRSLSAQVVD"
     gene            complement(1683..2306)
                     /gene="lepB"
                     /locus_tag="VN178_01980"
     CDS             complement(1683..2306)
                     /gene="lepB"
                     /locus_tag="VN178_01980"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWS79764.1"
                     /translation="MSHIRPGALSYYFMSEKPYYHNEQFQTSSEERRPATKKGGGALE
                     YLVILLVSFALVFGFVRPFVMEAFWIPSGSMIPTLEIGDRVLVNKFIYRFTDPGRGDI
                     IVFESVDNSNEDLIKRVVGLPGDRIAVRGGRLFVNGEPQKEPYTNKKMPDRSFYARTK
                     VPKNHVFVMGDNRGNSADSRVFGPLPEKNIEGEAFLRFWPPDRIGLL"
     gene            complement(2303..3091)
                     /locus_tag="VN178_01985"
     CDS             complement(2303..3091)
                     /locus_tag="VN178_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWS79765.1"
                     /translation="MDYLEFLFLGAIGVVGGVLSGLVGVGGGIVFVPGLLYVGGWNIQ
                     DAVAASLVIIIFSSLSGTIRNARTADPVNWRVAGLLSLAVAPSALIGVLISRVSPEVV
                     VKVVFATLLIALAYPTARGGGEYESDRKGIPVPLVFLAGILVGTLSGLVGVGGGVVMV
                     PLMVLGMGLTTKRAVSTSLAVVMFTGIVGAVGYVATGFRDVQDLLSLPPLIIGSVIGA
                     PLGVRLRDWLPEGVVRIGFGVFMVIVALHLFAQALGKFWEPRIF"
     gene            complement(3096..3710)
                     /locus_tag="VN178_01990"
     CDS             complement(3096..3710)
                     /locus_tag="VN178_01990"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWS79766.1"
                     /translation="MLEAILHPLIEFVTATIGSYGVPAVFVLMLLESMGILIPSEAIS
                     PFAGYLVSEGKMGFLAAVAAGVLGNVVGSWIAYFIGLWGGRELWFHYGRYVGVRAHHL
                     HVAEKWFDKYGEVTVFVSRCLPVVRTFISFPAGTARMNFTKFTAYTFLGCIPWVFALT
                     YFGYVLGENWTTIGDYLHYLDYAVAVALVAGAVYLFWRWRRSAA"
     gene            complement(3811..4530)
                     /locus_tag="VN178_01995"
     CDS             complement(3811..4530)
                     /locus_tag="VN178_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWS79767.1"
                     /translation="MLETRREIVRWIAERLTVVSAAWLAAGLAFSAFVIWAFTELVDV
                     VVEGDSRVFDRAALLWIHSTLPGWLDGPMRIVTALGYYWFVVALLVVVVVLFYRRGWK
                     LSAILLLVSTAGSAVLTTVLKSVFQRARPELFDSGYHASFYSFPSGHATVAVGLYGML
                     TLVLAYRLRGAARWAVVVSGIFVVLLIGFSRLYLGVHYPTDIVAGYLAALLWLVCVGA
                     VYALWLSVRSFRVAESSRGEG"
     gene            4618..6324
                     /gene="polX"
                     /locus_tag="VN178_02000"
     CDS             4618..6324
                     /gene="polX"
                     /locus_tag="VN178_02000"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HWS79768.1"
                     /translation="MQNNDIVRALETIATLMEIKDEAYYRVLGYTRAAESVAALGRPV
                     TEVDDLKTLPHVGATTAEVVRDLAEDRTPRLLAELTAEIPEGLVEITRLPSVGPRTVG
                     RLWKELGITSVEELAAVDPERISSLKGFGKKSAQKMVQAARTYNATERRMLLDEATSL
                     GEQILSFVRSHPATQRAEVGGSLRRMKETVGDIDIVAASEDPGSLADDFAQAPFAEEV
                     LAHGPKKVFILSGGTEIDLRIVEPEAYGSLLHHFTGGQAHNIVLRERAVKMGINISEY
                     GLARAGTGDYKPVPTEEDLYSRLGLAYIPPELREDAGEIEAADSGTLPDLISVEDIRG
                     DLHVHTNYSDGKGTIESMAEAAIGLGYEYLVFCDHSQSLRVANGLSPERLKEKLEAVR
                     KADEGYGEIHLLCGSEVDILRDGSLDYDDDVLAELDFVVASVHTSFGVGEKAMTERIV
                     RAMNNPYVRTIGHPTGRILNRREPYEVDVSRLIREAAATNTSLELNAYVDRLDLAVPY
                     VREAVGAGVSITIDTDAHDERALGFMKYGVSQARRAWVEKGSVINCLPFGKFEKYLKN
                     GK"
     gene            6443..7351
                     /locus_tag="VN178_02005"
     CDS             6443..7351
                     /locus_tag="VN178_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79769.1"
                     /translation="MILIRIPHVTLEIAHVKYVLSDRALLGCKHGSESGDEMIERLRR
                     LSPPSRYLIYVAGVLVVLVVAVGVGAAAAVVVGWQSGRAATGPAETSTLEGGKLETTT
                     AAKATEGTTAGSSSDRKNSDATANKASFVHRATDQNSRGDYTYIGDPSIDGDPNAIVI
                     VSPTPDQEKLGSASYNHNIGVWYEGGARKWAIFNQDRAAVPAGATFEVVVPRASAKFV
                     HEAGLVNTAGNYTYLDDRLTNGEPDAVLSVTQNWNPGGGRGVYNDHPVDTVYDAQVER
                     WAIYNRDNASIPRGAAFNVAVSAGAD"
     gene            7459..8616
                     /locus_tag="VN178_02010"
     CDS             7459..8616
                     /locus_tag="VN178_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79770.1"
                     /translation="MQLYGQGTDVSALLGVRLETQKMAGRTYTAFPNLTNVRWLLPAK
                     EPALRRAGIGGLYQPNSLRGRALRTIIGAGAVLGERVWLEEDALARLETTVASVMGVE
                     AVRLAFYVGVPSPHRKITAQVLTPEGKTLAYAKIATSSLAQAALESERRALLRLSESA
                     GLKGTVPEVLGTLPWHGGTILLITMGPPRPGPRRLSSVHLRFCTHLFRSFEVRETFAE
                     SPMWTRISEIWHHLRNSSPETLPANLGPALERLHDELGPVGMPLSIAHGDFTPWNTRL
                     GPQSLFVLDWERASEGMSPLYDAFNFQALQAALQGRRSGIRDRRLFLTLLDALWPEGR
                     KHLPGLYLAYLAHVGLLYSEAQSLDPGVGEKKVWGWFAQQIESVLSDDASL"
     gene            8687..9862
                     /locus_tag="VN178_02015"
     CDS             8687..9862
                     /locus_tag="VN178_02015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWS79771.1"
                     /translation="MKILAVHNRYQRPGGEDQVFVDETALLEARDHLVLRYEVHNDQV
                     ERVNRLTLAKDTVWNTPVYRELGALIRRERPDVVHFHNTLPLVSPAGYYAARAEGVPV
                     IQTLHNYRLLCPVALFFRDGRVCEDCMGKAVPWPGVVHKCYRGSRAASGVIAAMLTVH
                     RVLRTWTEMVDVYIALTEFARSKFIEGGLPAGKIVVKPNFVAPDPGRGRGGGGYALFV
                     GRLAPEKGTGTMLAAWERLGTRIPLKIVGDGPLRDRVVGAAKRSKVEWLGHRPVKEVH
                     ALMGKADMLVFPSEWYETFGRVAVEAFAVGTPVIAADIGAVAELVEHGRTGLVFRPGD
                     PEDLVTQVEWALSHPAELLRMRDEVRAEFEAKYTAERNYRALMEIYEAALERKKVPA"
     gene            9859..10632
                     /locus_tag="VN178_02020"
     CDS             9859..10632
                     /locus_tag="VN178_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWS79772.1"
                     /translation="MTLAFRHVLGMRVDATSYEDASQRVARWAHDGRSAYVCVATVHM
                     TMEAFDSPAFQRVVNGADLVTPDGRPLVWALRSLGVRDATQVRGTDLTTHVVERAAAR
                     EGIPIGLYGGTPDLLKTFVRILERRYPGVRVVCQIAPPFRPLTPEEDERVTEEIVSSG
                     ARILFVGIGCPKQERWMEAHKDRIPAVMIGVGAAFDFHTGRVRQAPGWMQAAGLEWLF
                     RLLMDPKRLWKRYAKHNPRFVVLFLRQLLGLRFGRKQGG"
     gene            10847..11827
                     /locus_tag="VN178_02025"
     CDS             10847..11827
                     /locus_tag="VN178_02025"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWS79773.1"
                     /translation="MATRVLVTGAGGFIGHHLVKYLVDQGYWVRGVDIKHPEYEPSPS
                     HEFELLDLRRFDNCLIATRGIDEVYHLAADMGGIGYITAFHADIARNSAMINIHMLEA
                     ARINAAERFLFSSSACVYPMYLQKSPDVTNLKEDDAYPAEPEEGYGTEKLFMEELCKY
                     YNEDYDFETRVVRFHNVYGPLGTYEGGKEKAPAAISRKVALAADGDEIEVWGDGEQTR
                     SFMHVDDCVEGIYRIMRSEYPHPLNLGTDELVTINELVDMVAEISGKRITKRHDLSKP
                     QGVRGRNSDNSRLREVLDWEPGIPLREGLTPTYRWIESELRIAGRVPQPA"
     gene            complement(11871..12770)
                     /locus_tag="VN178_02030"
     CDS             complement(11871..12770)
                     /locus_tag="VN178_02030"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79774.1"
                     /translation="MPNFFIVGAQKAGTTSLYQYLNQHPQVYMSPRKEPHFFEGMRSE
                     YRRPGRRSVPVSDLAEYKALFAGASDERAVGEASASYLYSPKAPGLIKSSVPDAKIIA
                     ILRNPADRAYSNFVYCVQVGREPLGSFAEALQAEEARTRDKWGPLWYYKQKGFYYAQV
                     KRYFDTFGRDQVGIWLYEDLRNDPLGTLREVFRFLDIDESFVPDASMEYNPSGVPRVG
                     RLYKGVRTLTARNPALVERVLPARLRGYVKRRIFVKPPPFPQEVRRQLTDSYGEDILR
                     LQQLIGRDLSAWLEDPRGSGSRE"
     gene            complement(12808..13731)
                     /locus_tag="VN178_02035"
     CDS             complement(12808..13731)
                     /locus_tag="VN178_02035"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS79775.1"
                     /translation="MTMPNFFIVGAQKAGTTSLYQYLNQHPQVYMSPIKEPFFFDHEM
                     DSNGGVVWREFEGHRQPPRFTSIEEYSALFKGVKDEKAIGEATPLYIYAPGTAERIER
                     FVPGAKPIALLRNPADRAYSAFLYALRMGVEPLTDFAQALREEPFRIRNRWHYVFHYR
                     SRGLYHQQLKRYYEVFGRERLGVWLYEDMREDPAGVARSVFRFLGVDDTFAPDTSSRY
                     NPAGVPASRAARAAMRATNRAVWVLRKALPPTSGILPLAFEMRRAVQSRVLTEPPPID
                     PELRGELVEGYREDILRLQELIGRDLSIWLR"
     gene            14029..14814
                     /locus_tag="VN178_02040"
     CDS             14029..14814
                     /locus_tag="VN178_02040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWS79776.1"
                     /translation="MNGEKGDWPKISVVTPSFNQGQYIEATIKSVLDQGYPNLEYIIM
                     DGGSTDNTVEILKKYSDRLAFWVSEPDEGQTDALIKGFDRAGGDILCWLCSDDLFEAR
                     TLQEVAETFVEHPDWEVVYGDSFWIDADSRPIRFKREIPYYRFIWMYDHNYLPQPSTF
                     WRRGIYEKVGGLDARMELSMDADLWSRFAEHTALHHVPLAWSRMRYYPEQKNRRLRSK
                     SDEEDRVIRSRYLPHEPRWLLSLKWFLAKGLRVVLKLARRAYW"
     gene            15076..>16060
                     /locus_tag="VN178_02045"
     CDS             15076..>16060
                     /locus_tag="VN178_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWS79777.1"
                     /translation="MAVSVLTAIMLALPEGAIALPSEVPDDTPMVDGRVRAIEQVGTN
                     IWVGGRFTKVKQRDGTVLANVANVAVFDSQTEQYKPIAPSLGGTGAEVWDMMVYGGTG
                     PHADDVLIAGKFSGPSTTKKNLVLVDGTTGEVLWWYNAPTLKSVLAAPNLSAPGGDGR
                     VYGGGVSLSAFDFATGKKLWTKAKTEVDATIRTHDSSPAYRDLELDADGQTIWAACVC
                     DWVGAAPAKAMVKLNIEGVHDASWVTEAGKAALGQSVVDHDGKLYLAAGGSDFLAEYD
                     KGTDGARGWVRDTSGSAQAVELVDGKLVVGGHFYAVGDQGGDRCGAGRPGDV"
BASE COUNT         3220 a         4835 c         5217 g         2788 t
ORIGIN      
        1 cgccgacttc gtggtcgagc gggtacggga cgaagcgggg cttgcggcat ggatcgaagc
       61 gctcggctcg ggcttcgggg cggggcccgt agaagcggag tgggctgggg agatgtaccg
      121 cagattgggt ctcggcgccg aagggccgtg gcgccactac gtcgggctat tggccggtga
      181 gcctgtggcg acgtccacat cctttttcgg ggccggggtc gcggggatct acttcgtgtg
      241 caccgtcgaa cgcgccaggc ggcgggggat cggggcggga atcacactag cgccactgca
      301 cgaagcaaga gatctcggcc acacggtcgg tgtcctcggt tcctcggaga tgggctatcc
      361 cgtctaccgt aggctgggct tcgaggaata ctgcaggatc gggctctacg agtggcgttc
      421 tgggtagcgc ccacacgggg caccggatac ggtagcatgt gcgttcgacg ttagggcgaa
      481 taccaataga ggggatttcg atggcaagca aatcttacga ccgggcgcct gagatggcgc
      541 tcgagcctgg ctacgagtac tacgcgaact tcgttaccga gaagggtcct gtgaaggtga
      601 ggctcttcgc cgaggaggcg cccgagaccg tcaacaactt cgtcttcctg gcgcgcgacg
      661 gatatttcga cgggacgacg tttcaccgcg ttatagcgga ctttatggtc cagggcgggg
      721 acccgacggg gactgggacc ggcgggcccg ggtacaggat cccggacgag ttccatcccg
      781 ggttgcggca cgacaagccc ggcgtactct cgatggcgaa cgcgggcccg aacaccggcg
      841 ggtcgcagtt ctttatcacg cacgtggcga cgccgtggtt ggacgacagg cacgcggtct
      901 tcggagaggt cgtcgacggt atggaagtcg tcggtgccat aagggagcgg gacccacagc
      961 gcgacccgga gccgggcgac aggatagagc ggatcgagat cgaagagcta ccccggacgt
     1021 gaaccctcac tgagtgtaga tttacagttc catcgatggg tgggcgatac cctcctcgag
     1081 gaactgaaac cgcatgagag gtggtctcgt gtccaaagaa gtggccgaca ggttcgttga
     1141 ggcgctccgg aagctggagg aagacaggga cgtcgatgcc ctggtcgaga tccacgccca
     1201 ggactgcgac gtgggcaacg tcgccgtgcc gaaaaccttc tcgggccacg acggcctgcg
     1261 cgagttctgg acgggctacc gcgacacctt cggcgagatg aagagcgagt tccagaccgt
     1321 cttcgccgac gatgcgggcc acgccgcgct ggagtggaac acctcgggga aggccaacgg
     1381 caacgacgtc tcctacgacg gcgtaagcct cctggagata gaagaaggca aggtcacgcg
     1441 ctttcgggcc tatttcgacc cccgctccct cagcgcacag gtggttgatt agctggtcag
     1501 cacggcgctt cgcgccttgt cagcatttca gcctgtcagc ttctcctgtt ccggcctcac
     1561 ccagctgcgc acgaggaacc accactagtg gtggtcaggt cctacgctcg cggcgagact
     1621 acgggacaac ttgctgacat gctgacaagc gaaggctaaa agcctgagcg tgctgaagtg
     1681 ctctaaagca atccgatacg gtccggaggc cagaaacgta ggaaggcttc gccctctatg
     1741 ttcttctcgg gcaatggtcc gaagactctc gagtccgcgg agttgccacg gttatcaccc
     1801 atcacgaaga cgtggttctt ggggaccttg gtccgggcgt agaagctcct gtcgggcatc
     1861 ttcttgttcg tgtacggctc cttctggggc tcaccgttca cgaagagcct gccacccctt
     1921 acggcgatcc tgtctcccgg cagtccgacg acccgcttta tcaggtcctc gttggagtta
     1981 tcgacgctct cgaagactat gatgtctccg cgaccggggt ccgtgaagcg gtagataaac
     2041 ttgttcacga gcacccggtc gccgatctcg agcgtcggga tcatgctgcc cgaggggatc
     2101 cagaaggcct ccatgacgaa cggcctgacg aagccgaaga ccagggcgaa ggagaccagc
     2161 agtatgacca ggtactccag cgcccctcca cccttcttgg tggccggccg acgctcttcg
     2221 ctggaggtct ggaactgctc gttgtggtag tacggcttct cggacatgaa gtagtaggat
     2281 aacgcaccgg gacggatatg actcaaaaga ttctgggttc ccagaatttg ccgagcgctt
     2341 gggcgaagag gtggagggcc acgatcacca taaacacacc gaaaccgatc cgcaccacgc
     2401 cctccggaag ccagtccctg aggcgtaccc cgaggggggc gccgatcacg gaaccgatga
     2461 tgaggggcgg caggctgagc agatcctgca cgtccctgaa cccggtcgcc acgtaaccga
     2521 ccgcccctac gatacccgtg aacatcacga cggcgaggct cgtggagacg gcccgcttgg
     2581 tggtgagtcc catgccgagc accatcagcg ggaccatcac caccccgccg ccgaccccga
     2641 cgagccccga gagcgtccct accaagatgc cggccaggaa gaccaggggg acgggaatac
     2701 ccttgcggtc actctcgtac tcgcccccgc cacgggcggt cgggtaggcg agggctatga
     2761 gtagcgtggc gaacaccacc ttcaccacga cctcggggga gacgcggctt atgaggaccc
     2821 cgatcagagc cgaaggggct accgccaggg agaggaggcc ggcgacccgc cagttcacgg
     2881 ggtcagcggt cctggcgttg cgaatcgtgc cggagaggga gctgaagatg atgatcacca
     2941 ggctcgccgc aacggcatcc tggatgttcc atccgccgac gtacaacaga ccggggacga
     3001 agacgatacc tccaccgacc ccgacgaggc cggagagtac ccccccaacg accccgatgg
     3061 ccccaaggaa caagaactcc aggtaatcca ccgcgctagg ctgcggagcg ccgccagcgc
     3121 cagaacaaat aaacggctcc ggccaccagc gccacggcga cggcgtagtc gaggtagtgc
     3181 agataatcgc ctatcgtcgt ccagttctcg ccgagcacgt acccgaagta cgtcagcgcg
     3241 aagacccagg gtatgcaccc caggaaggtg tatgcggtga acttggtgaa gttcatcctg
     3301 gccgtgccgg cggggaaaga gatgaaggtt cggaccaccg gcaggcagcg ggagacgaag
     3361 acggtgacct caccgtactt gtcgaaccac ttctcggcga cgtgcaggtg atgcgccctg
     3421 acacccacgt atctcccgta gtggaaccag agctcgcgcc cgccccagag cccgataaag
     3481 taagcgatcc aggagcctac cacgttcccc aagaccccgg cggccaccgc cgcgaggaac
     3541 cccatctttc cctccgagac caggtaaccc gcgaagggtg agatcgcctc cgaggggatg
     3601 aggatcccca tgctctccag caacatcagc acgaagaccg cggggacgcc gtagctgccg
     3661 atggtcgcgg tgacgaactc tatgagcgga tgtaggatcg cttcgagcac ggtgagaagc
     3721 taccacttaa tgacacggat gtacaacgtc tatccgccat cttccgccag aaacccgaag
     3781 ataaagcatg caatccctga gggtccggta ctacccttcg ccgcggctcg actcggccac
     3841 cctaaagctc cggacggaga gccacagcgc gtacacggca ccgacgcaga ccagccagag
     3901 gagggcggcg aggtagccgg ccactatgtc cgtcgggtag tgcaccccga gatagagcct
     3961 ggaaaagccg atcaggagca cgacgaatat tcccgagacg accacggccc atcgcgctgc
     4021 cccccgtagc cggtaagcca ggacgagcgt gagcatcccg tacaacccga cggcgacggt
     4081 ggcgtggccg ctcggaaacg aatagaagga ggcgtggtag ccggagtcga agagttcggg
     4141 acgcgcccgc tggaatacgc ttttcaggac ggtggtgagc acggcgctac cagcggtcga
     4201 gaccaggagg agtatggccg agagcttcca gcctcttcgg tagaagagaa caacgaccac
     4261 gaccaggagc gctaccacga accagtagta gccgagcgcc gtgacgatac gcatcggtcc
     4321 gtcgagccag ccgggtaagg tggagtgtat ccagagcagc gcagccctgt cgaagacccg
     4381 gctatcgccc tcgacgacca cgtcgaccag ctcggtaaaa gcccagataa cgaaggccga
     4441 aaacgcgagc cccgcagcaa gccaggcggc ggagaccacc gtcaggcgct ctgcgatcca
     4501 ccggactatc tctctccggg tctcgagcat ggaggaattc taacccgttt cggtaccctg
     4561 gccgggcgta taatctctgt ggcggacggc cggtcctgag aaggaggagc gagcaccttg
     4621 cagaacaacg acatcgtccg ggcgctagag actatcgcga cgctcatgga gatcaaagac
     4681 gaggcgtact accgcgtgct cggctacacg cgggccgccg agtcggtggc cgccctcggt
     4741 cgaccggtga ccgaggtcga tgacctcaag acgctgcccc acgtcggcgc gacgacggcc
     4801 gaggtggtaa gggatctggc cgaagacagg acgccccggc tactcgccga gctgacggcc
     4861 gagataccgg aaggcctggt ggagataacg cgcctgccca gcgtcgggcc gcgcacggtc
     4921 gggcgcctgt ggaaggagct tggcatcacc agcgtcgagg agctcgcggc cgttgatccg
     4981 gagcggatct cctccctcaa gggcttcggg aagaagagcg cccagaagat ggtgcaggca
     5041 gcgaggacct acaacgcgac ggaacgccgg atgctcctgg acgaagcgac ctctctgggg
     5101 gagcagatcc tctcgttcgt ccgctcgcat ccggcgacac agagggccga ggtcggtggc
     5161 tcactacgcc gcatgaagga gaccgtcgga gacatcgata tcgtcgccgc aagcgaggac
     5221 cccggctcgc tcgccgacga cttcgcacag gcgccgttcg ccgaggaggt cctggcccac
     5281 ggcccgaaga aggtctttat cctgagcggc gggaccgaga tagacctgag aatagtcgag
     5341 cccgaggcct acggctcgct cttgcaccac ttcaccggcg gccaggccca caacatcgtg
     5401 ctgcgcgagc gggccgtcaa gatgggcatc aacatctccg agtacggcct ggccagggca
     5461 gggaccggcg actacaaacc cgtccccaca gaagaggatc tctactcgcg gctcggcctc
     5521 gcctacatcc ctccggagtt acgcgaggac gccggtgaga tcgaagccgc agacagtggc
     5581 actctgcccg acctcatctc cgtagaggat atccgcgggg acctgcacgt tcacaccaac
     5641 tactcagacg gcaaaggcac catcgagagc atggccgagg cggccatcgg actcggctac
     5701 gagtacctcg tcttctgcga ccactcccag agcctcaggg tggctaacgg cctctccccc
     5761 gagcgcctga aggagaagct ggaggccgtg agaaaggccg acgagggcta cggcgagatc
     5821 cacctcctct gcggctccga ggtcgacatc ctaagggacg gctccctcga ctacgacgac
     5881 gatgtgctcg ccgagctgga cttcgtcgtt gcttccgtgc acacctcttt cggggtcggc
     5941 gagaaggcca tgacggagcg catcgtgcgg gcgatgaaca acccgtacgt gcggactatc
     6001 ggccacccca cgggccgcat cctgaaccgc agggaaccct atgaggtaga cgtctcacgc
     6061 ctcataaggg aggccgcggc cacgaacact tctctcgagc tcaacgccta cgtggaccgc
     6121 ctcgacctcg cggttcccta cgtccgcgag gccgtggggg caggcgtcag catcacgatc
     6181 gacacggatg cccacgacga gcgggccctc ggcttcatga agtacggcgt ctcgcaggcc
     6241 agaagggcct gggtcgagaa ggggagcgtg atcaactgcc tccccttcgg gaagttcgag
     6301 aagtacctga agaacgggaa gtgatcttcc ctgtgcaagg ttcttctctg accttgccta
     6361 acgttacgat tcatcgggcc ggaccctaca ggtccgtggc acggccgatc cacctggtgg
     6421 gacgcaaaga gctcatccag ggatgatcct catccggatc ccacacgtaa cactcgagat
     6481 agcccatgta aagtatgtac tttccgaccg cgcgctacta ggatgtaagc acggatcgga
     6541 gagtggagat gagatgatcg agcggctaag gaggctttca cccccatcaa ggtacttgat
     6601 atacgtcgct ggtgtgctcg tggtgcttgt cgtggctgtc ggcgtaggcg cggcggctgc
     6661 ggtcgttgtc ggctggcagt ccgggcgggc agcgacgggc cccgccgaga ccagcacact
     6721 cgaaggcggc aagctggaga cgaccaccgc tgccaaagcg acagaaggca cgaccgccgg
     6781 gtcgtccagc gatcgcaaga actccgacgc cacggccaac aaggcctcct tcgtccatcg
     6841 ggctacggat cagaacagcc gcggagacta cacctatatc ggcgacccga gcatagatgg
     6901 ggatccgaac gccatcgtca tcgtctcacc gaccccggat caggaaaagc ttgggtctgc
     6961 ttcatacaat cacaacatcg gtgtctggta cgagggcggg gcaaggaagt gggcgatctt
     7021 caaccaggat cgagcagcgg tgccagcagg agccaccttc gaggtggtcg ttccgcgagc
     7081 atccgcgaag ttcgtccacg aagcaggtct cgtgaacacc gccgggaact acacctacct
     7141 cgatgatcgt cttaccaacg gcgaacccga cgccgtactc tcggtcacgc agaactggaa
     7201 tcccggaggc ggcagggggg tatacaacga ccatccggta gataccgtgt acgatgcaca
     7261 ggtggaaaga tgggcgatct acaaccgaga caacgcatcg atacccaggg gagccgcctt
     7321 caatgtcgcc gtctcggcgg gcgccgacta gcgtgcgaag tagcaactct gcggcggatt
     7381 tcacctccca tgccgtcgtg cgggtgagcc agaggagagc cgcgtggata atctgatccg
     7441 gcaagaagca gcttaggtat gcagctttac gggcagggaa ccgatgtttc cgcgctcttg
     7501 ggagtcaggc tcgagaccca gaagatggcg ggacgcacgt acacggcctt tccgaacctc
     7561 accaatgtcc gctggctgct gccggcgaag gagcccgctt tgcgccgggc aggtatagga
     7621 gggttgtatc agcccaacag tctgcggggc cgggcactac gaacgatcat aggcgccgga
     7681 gccgtgctgg gtgaaagggt atggctggag gaagacgccc tggcgcgttt ggagacgacg
     7741 gtggcctccg tcatgggagt ggaggcggtg cgtctggctt tctacgtggg tgtaccatcc
     7801 ccacaccgga aaatcaccgc gcaggtgctc acccccgagg ggaagacgct ggcttacgcc
     7861 aagatcgcca cttcgtcact ggcccaggcg gctctcgaat ccgaaaggcg tgccctgttg
     7921 cggttatcgg agagcgcggg cctcaaaggc acggtcccag aggtcttggg cactcttccc
     7981 tggcacgggg gcactatact cctcatcacg atgggccccc cgcgtccggg tcccagaagg
     8041 ctgtcatccg tgcacctgcg tttctgcacg catctctttc ggtccttcga ggtacgggaa
     8101 acgtttgcgg agagtccgat gtggacccgc atatccgaaa tatggcacca ccttcgcaat
     8161 agctctcccg aaaccctgcc tgccaatctt ggtccggccc tcgagaggtt gcacgacgag
     8221 ttggggccgg tgggtatgcc tttgtctata gcccacggcg acttcacacc ctggaatacc
     8281 cgattgggac cgcaatccct gttcgtactc gattgggaac gtgcctccga aggaatgagt
     8341 cccctgtacg acgcgttcaa cttccaggcc ctgcaggccg ccctgcaggg gcggcgtagt
     8401 ggcattcggg atcgccggct ttttctgacc ctgttggacg ccctgtggcc ggaaggacgg
     8461 aaacatctgc ccggtctgta tcttgcttac ctggcacacg tcggcctgct ctacagcgag
     8521 gcacagagcc tggaccccgg cgtcggagag aaaaaggtct ggggttggtt cgcgcagcag
     8581 atcgaaagcg tcctgagcga cgatgcgtca ttgtagtcga ggacgcagcc gtagcccgca
     8641 ttttcccacc atgccctgaa aatgcgggta tggtaacttg tgccagatga agatcctggc
     8701 ggtccacaac aggtaccagc gacccggcgg cgaagaccag gttttcgtgg acgaaaccgc
     8761 tctcctggag gcccgagatc acctggtgtt gcgttacgag gtccacaacg accaggtcga
     8821 gcgagtgaac cgtctgaccc tggcgaaaga tacggtatgg aacactcctg tgtatcgtga
     8881 actaggggct ttgatccgcc gggagcggcc cgacgtggtc cactttcaca acaccctgcc
     8941 cctggtctcc ccggccggct actacgccgc cagggccgaa ggtgtgccgg taatacagac
     9001 cctgcacaac taccgtctgc tctgtccagt agccctgttc ttccgtgatg ggcgggtgtg
     9061 cgaggattgt atgggcaaag cggtcccctg gcccggggtc gttcacaagt gctaccgggg
     9121 gagccgggcg gcgagcgggg tgatagcggc gatgctcacc gtgcaccgtg ttttgcgtac
     9181 atggacagag atggtcgatg tctacattgc ccttacggag tttgcccgga gtaaattcat
     9241 cgagggcggc ctccccgcgg ggaagatcgt ggtgaaaccc aacttcgttg cccccgatcc
     9301 tggtcggggc cggggaggag gcgggtacgc cctgttcgtg ggccggctcg cgcccgagaa
     9361 aggaacgggg acgatgctgg ccgcctggga aaggctcgga acgaggatcc cgctcaagat
     9421 cgtgggagac ggcccattga gggatcgggt agtaggagcc gccaagcgat ccaaagtgga
     9481 gtggctggga cacaggccgg taaaggaagt ccacgcgctg atggggaagg ccgacatgct
     9541 cgtcttcccc tcggagtggt acgagacgtt cggccgggtg gcggtagaag ctttcgcggt
     9601 gggcactccg gttatcgcgg ctgatatcgg agcggtcgcc gagctcgtag aacacggccg
     9661 cacggggctc gtattccgcc ccggcgaccc ggaggatctc gtcacacagg tcgagtgggc
     9721 cctgtcgcac ccggcagaac tactacgtat gcgtgatgag gtcagggccg agttcgaggc
     9781 gaagtacact gcggagcgca attaccgggc gctgatggag atctacgagg ccgccctgga
     9841 gcgcaagaaa gtgccggctt gaccctggcc ttcaggcacg tgctcggtat gcgggtagac
     9901 gctacctcct acgaggacgc ttcacagcgc gtggcgcgtt gggcgcacga tggacgctcg
     9961 gcgtacgtgt gcgtggcgac cgtgcacatg accatggagg cctttgactc cccggcgttt
    10021 cagcgggtgg taaacggtgc cgacctggtt acgcccgacg gaagacctct ggtttgggcg
    10081 ttgaggagcc tgggggtcag agacgcaacc caggttcggg ggacggacct gacgacgcac
    10141 gtggtggaac gggccgccgc gcgggaaggc ataccgatcg ggctctacgg cggcacgccg
    10201 gacctgctaa agacgttcgt tcgcatcctg gaaaggcgct accccggggt gcgggtagtg
    10261 tgccagatcg caccgccttt ccgtcccttg acgcccgagg aagacgagag ggtaactgag
    10321 gagattgtct cctcgggtgc gcgtatcctg ttcgtgggta tcggctgtcc caaacaggag
    10381 agatggatgg aggcgcataa ggatcgcatt ccagcggtga tgatcggtgt tggcgcagcg
    10441 ttcgacttcc acacgggtcg ggtgcgccag gccccgggct ggatgcaggc ggcaggcctg
    10501 gaatggttgt tcaggttgct gatggatccc aagcgtttgt ggaagcgtta tgccaaacac
    10561 aacccgagat tcgtcgtgct gttcttgcgg cagcttctgg ggctacggtt cggtagaaag
    10621 caaggcgggt aggctagaga ggacgcagtg gtagcttcgg cgtacgccct ttcgtgcgaa
    10681 ccaacacgcc tgagaaagtt gggagacagg cggcgaaatg tgcccggtgg acgacgcaag
    10741 gggtctcggc gaatcccgtt acgtcgagga tacgatgtga acgtttcggt atcttctagt
    10801 atattgaggt ttagaagtga tcgtttcgtg atgttgggag gaacacatgg caacacgggt
    10861 cttagtgaca ggcgccggcg gctttatcgg gcaccatctg gtgaagtacc tggtggatca
    10921 gggctactgg gtgcggggcg tcgacatcaa acaccccgag tacgagccga gtccgagcca
    10981 cgagttcgag ctcctggatc tccgccgctt cgacaactgc ctgatagcca cccggggcat
    11041 cgacgaggtg taccacctgg cggcggatat gggtggcatc ggctacatca ccgctttcca
    11101 cgccgatatc gcccgcaaca gcgccatgat caacatccac atgcttgagg cggcccggat
    11161 caacgcggcc gagcgattcc ttttctcctc ctcggcctgt gtttacccga tgtatctcca
    11221 gaagagcccg gacgttacca acctgaagga agacgatgcc tacccggcgg aacccgagga
    11281 ggggtacggc accgagaagc tctttatgga agagctctgc aaatactaca acgaggacta
    11341 cgacttcgag actcgggtag tccgcttcca caacgtctac gggcctctcg gcacctacga
    11401 agggggcaag gagaaggccc cggcggccat cagccgcaag gtggccctgg ccgccgacgg
    11461 ggacgagatc gaggtctggg gcgacggcga gcagacccgc tcctttatgc acgtagacga
    11521 ctgcgtagag ggcatctacc ggatcatgcg ctcggagtac ccccacccgc tcaatctcgg
    11581 taccgacgag ctggtgacca tcaacgaact ggtcgacatg gtggccgaga tctccggcaa
    11641 gcgcatcacc aagcgccacg atctctccaa gccgcaaggg gtgcggggcc gaaacagcga
    11701 caacagtaga ctccgagagg tgctcgactg ggagcccggt atccccctga gggaaggtct
    11761 cacgcctacc taccgttgga tcgagtcgga gctcaggatc gcaggaaggg tcccccagcc
    11821 cgcctagagc ggctcgtaga aggccttcca cctcgctcgg ggagtaggtc ctactcccta
    11881 ctccccgacc ctcgcgggtc ctccagccac gccgagaggt cccgcccgat gagctgttgc
    11941 agcctcagga tgtcttcccc gtaggagtcg gtcaactgcc ggcgcacctc ttgcggaaac
    12001 ggcggaggct tgacgaagat acggcgcttg acgtacccgc gcagtctggc ggggaggacc
    12061 cgttcgacga gggcggggtt gcgcgcggtc agtgttctca cccctttgta caatctcccg
    12121 actctcggta ctccagaggg gttgtactcc atggacgcat cgggaacgaa actttcgtcg
    12181 atgtccagga aacggaagac ctcgcgtaga gttcccaggg ggtcgtttct caggtcttcg
    12241 tagagccaga ttccgacctg gtcccgaccg aaagtgtcga agtagcgctt tacctgggcg
    12301 tagtagaaac ctttctgttt gtagtaccag aggggtcccc acttatcccg cgtacgcgcc
    12361 tcttccgcct gcaaagcctc ggcgaagcta ccgagcggtt cgcgaccgac ctgtacgcag
    12421 tacacgaaat tggagtacgc cctatcggcg gggttgcgaa ggatcgcgat gatcttggcg
    12481 tctgggacag agctcttgat gaggccaggg gccttggggc tatacaggta ggaggcggag
    12541 gcctcgccga ccgctctctc gtctgaggct cccgcgaaaa gggccttgta ctcagcgagg
    12601 tcgcttacgg ggacgctcct gcgacccggt ctgcggtatt ccgagcgcat gccctcgaag
    12661 aaatgcggtt ccttgcgggg gctcatgtag acctgcgggt gctggttgag gtattggtag
    12721 agcgaggtcg tccccgcctt ctgcgcgcct actatgaaga aattgggcat cgtcacgcgc
    12781 gtaaaccccc ttgctcgcgg cccggcctta tcgtaaccat atcgagaggt cccgcccgat
    12841 gagttcctgc agcctaagga tgtcttccct gtagccttcg accagctccc cgcggagctc
    12901 cggatcgatc ggcggcggct cggtgaggac ccggctctgc accgctcgac gcatctcgaa
    12961 ggcgagcggc aagatgccgg acgtgggcgg aagggctttc cggaggaccc agactgccct
    13021 gttcgttgcc ctcatcgcgg cgcgggcggc cctgcttgcg ggcacgccgg ccgggttgta
    13081 cctcgaggaa gtgtccggag cgaaggtgtc gtcgaccccg aggaagcgga agacactccg
    13141 ggccacgccg gcgggatctt ctctcatgtc ttcgtagagc cacaccccga gcctctctcg
    13201 cccgaaaact tcgtagtaac gtttcaactg ctggtggtag agccccctgc tgcggtaatg
    13261 aaatacgtag tgccagcggt tacgaatacg gaacggctct tcgcggagcg cttgcgcgaa
    13321 gtcggtgagt ggctctaccc ccatacggag ggcgtacagg aaagccgaat acgccctgtc
    13381 ggcgggatta cgcaggaggg cgatcggctt cgcccctggt acgaatctct ctatgcgctc
    13441 ggcggtcccc ggagcgtaga tgtacaaggg ggtcgcctcg ccgatcgcct tctcatcttt
    13501 cacccccttg aacagcgcgc tgtactcctc gatactggtg aacctcggag gttggcgatg
    13561 cccctcgaac tcgcgccata cgaccccgcc attggaatcc atctcgtggt cgaagaagaa
    13621 aggctctttg atggggctca tgtagacctg cggatgctgg ttgaggtatt ggtagagcga
    13681 ggtcgtcccc gccttctgcg cgcctactat gaagaagttg ggcatcgtca tctgattacc
    13741 tcacccgcta gtcctcgcaa gaatgcttcg tcgtagatct cgatactatc caaggaatgc
    13801 cgccgggcta ggatgccacc cctcccgggc tctgtcaatg atcttgcggg gcccctcgac
    13861 cttgggccgc ccgggtcaaa cgcgcatcgc gcggttaaat gagatcggct ggtaatcggc
    13921 acgaatgtct actacgggta tgttagggtg ggaatcgaca gcgggtgagt ggacctaccg
    13981 agatatcgtg gagtactgtg agatcgtaga tgggagctgg atcgtagtat gaatggcgaa
    14041 aagggagatt ggccgaagat atctgtggtg acgccttcgt tcaaccaggg tcagtacatc
    14101 gaggcgacga tcaagagcgt actagatcaa ggttatccca acctggagta cataattatg
    14161 gacggtggct cgaccgacaa caccgtagag atcctgaaga agtactccga ccgactggct
    14221 ttctgggtga gcgaaccgga cgaagggcag accgacgcgc tgataaaggg gttcgaccgg
    14281 gcgggcggcg acatcctgtg ctggctgtgc tccgacgacc tgttcgaagc ccgcaccttg
    14341 caagaggtgg ccgagacctt cgtcgagcat cccgactggg aggtggtcta cggtgacagc
    14401 ttctggatag acgccgatag ccgtcccatc cgtttcaaga gggagatccc ctactaccgt
    14461 tttatctgga tgtacgacca caactacctg ccgcagccat ccacgttctg gcgtcgcggt
    14521 atctacgaga aggtgggcgg cctggatgcc cggatggaac tgagcatgga cgccgacctg
    14581 tggtcccgct tcgccgaaca caccgccctt catcacgtcc ccctggcctg gtcccggatg
    14641 cgctactacc cggagcaaaa gaaccggcgt ctgcgctcca agagcgacga ggaggatcgc
    14701 gtgatccgaa gtcgctatct gccccacgaa ccccggtggc tcctcagcct gaaatggttc
    14761 ctcgccaaag gcctgcgggt cgtcctgaag ttggcccgaa gggcctactg gtaacccgct
    14821 ggaagccggc aagagcgagt accttcgatg gacgagcaag gtttccccgg gcggcaaggc
    14881 cgtgagaaga agcgaagaca gaagtaaccc gaatgattag cgctgtactc gagtgtgata
    14941 gaatgcggca ctatcttcca ggtcttacat ttcgcgggat tacactgtgc aagcggtgct
    15001 tgtacggtat tcagaagggg ggacacagtg gggaatgaat gggattgcaa cacgaagagg
    15061 acgctccttg ccgccatggc ggtgtcggtt ttgacggcca tcatgctggc actacccgag
    15121 ggcgccattg cgttgccctc cgaggtcccc gacgacaccc ccatggtcga cggacgcgtc
    15181 agggctatcg agcaggtggg caccaacatc tgggtgggcg gccgtttcac caaggtcaag
    15241 cagcgcgacg gcacggtctt ggccaacgta gccaacgtgg cggtcttcga ctcgcagacg
    15301 gaacagtaca agcccatcgc gccgagcctc gggggcacgg gggccgaggt gtgggacatg
    15361 atggtgtacg ggggaacggg gccgcacgcc gacgacgtgc tgatcgccgg caagttctct
    15421 gggcccagta ccaccaagaa gaacctggtt ctggtcgacg gcactaccgg agaagtactc
    15481 tggtggtaca acgcgccgac gctgaagtcg gtcctggctg cgcccaacct gtctgcgccc
    15541 ggcggggatg gaagggtcta cggcgggggg gtgagcctct cggcgttcga cttcgccacc
    15601 ggcaagaagc tttggaccaa ggcgaagacc gaggtagacg ccaccatacg cacccacgac
    15661 agcagtccgg cctatcgcga cctcgagctc gacgccgacg gccagacgat ctgggcggct
    15721 tgcgtctgcg actgggtggg cgctgccccg gccaaagcca tggtcaaact caacatcgag
    15781 ggtgtccacg acgcctcgtg ggtgacggag gcggggaaag cggcattagg acaatcggtg
    15841 gtggaccacg atgggaagct ctatctcgcg gccgggggca gcgactttct ggccgagtac
    15901 gataaaggga ccgatggcgc ccgcggctgg gtgcgcgaca cctcgggctc ggcacaggca
    15961 gtggagctcg tggacggtaa actcgtggtc gggggacact tctacgcggt cggggatcag
    16021 ggcggcgacc gttgtggggc ggggcgcccc ggggacgtcg
//
LOCUS       DATMVF010000045        13770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_327804, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000045 DATMVF010000000
VERSION     DATMVF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327804"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="VN178_02050"
     CDS             <1..1706
                     /locus_tag="VN178_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWS79778.1"
                     /translation="LVLRIGSTNDIDGFNPFKIVEIPSYEVMSLTYDYLVDFSPKDSS
                     PVPGLADSWETSDDGLTWTFHLNKDAKWHDGKPVTSEDVAYTFNRILDEEQGLYIDYV
                     RQIETIETPDRHTVVFETKDPSVQMLSMLVYILPKHIWEDVPADETKTFKNSPAIGSG
                     PFQAVEWKEGQSVRLEANPDYFRSAPHIDEIIFQLYDNADTMVQALKNGEVDYIQPVP
                     INLFKSLENQEGIETNSAPDPGFTELGFNLYEPSPEAIEEFDAPKTSTGNPALLDPQV
                     REAINWSIDEEELTQKILQGEGIPGSTVVPPALAKYHLRLDESEIQGFDIERSKDLLR
                     ESGWRDTNGDGTVDKAGEELELRLFVRSEDKDSVTAGQFIEDWFGEAGIGLETKAISD
                     TALTDAIYAADYDMFIWGWGSDPDPDFILSVLTCDQIMGWSDTFWCNDDYSRMYQEQK
                     EQLDIDERAATIKEMQRIAYEENPYVIFYYDNQTEAWRTDKFEGWTKTPTAADPGQVA
                     FTFTTKTYLNLQPLSSQDEAGSGGGGTSPLLYVLLAVVAVAVIAGVVFFLRRGSAEDR
                     A"
     gene            1703..2680
                     /locus_tag="VN178_02055"
     CDS             1703..2680
                     /locus_tag="VN178_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79779.1"
                     /translation="MSRARYVAVKLIGAVGTLFFVLVLNFFLFRILPGDPERTLTRLQ
                     RVSPSTIDEVKREFGLDRPLTVQFFDYLGDTARGELGISYVFSRPVSEVIGTALWPTI
                     LLVGVATVAATVVGMLMGIWGAWRRGSAVDTSLLGFGLVTYAMPEFWLGMLLILVFAV
                     ALDWFPTAFMTTPGLTDASAGVQLVDTLEHLFLPALTLGLALLGEYALIMRSSVIEAM
                     NEDYVTTARAKGMREALVLRKHVVPNALLPVVTLAALNLGFIVSGAITVETVFSWPGL
                     GQLTFRAINGGPDYPLLQGLFLLFSVAVIFANLAADLLLGLLDPRIGRV"
     gene            2677..3582
                     /locus_tag="VN178_02060"
     CDS             2677..3582
                     /locus_tag="VN178_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79780.1"
                     /translation="MSAQTTRSGRAVRRTRRIQAFKTSASLFARNKMGLAGLVILTVF
                     TLTALFADVLVPEARLSVINAPGTPLEPPSAQFWLGTDELGRSVLDLVIQGSQISLLV
                     GFLATAISMVIGALVGITAGYFGGWTDVILMRLTDWFLVIPFLPLAIVLAAILGRSLF
                     VIALVIGVTTWPATARVVRAQVLTLKERPYVERARALGAGDRQIIGRHILPNVSPLIF
                     ANTILIVALAIYTEAILSFLGLGDPFRVSWGSILESAFTEGAISLGAWWYLVPPGLCI
                     VLVVLGFTMCGYAFDEILDPRLRDR"
     gene            3579..4571
                     /locus_tag="VN178_02065"
     CDS             3579..4571
                     /locus_tag="VN178_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS79781.1"
                     /translation="MSETAAPAGTLLEIRNLRVLYEVRGAKAPAVDGVDLTLRPGEAL
                     GLAGESGCGKSTLALACLGLLPRNATVEGSIRFQETEVIGAKPGRLRALRWAGISMVF
                     QGAMNAFNPVKRVGDQVIEAILLHEDVSRSEARSRMQELFEHVGIAPRRAGEYPHEFS
                     GGMRQRVMIAMALACDPPLVIADEPTTALDVMIQAQILNLLAQLREESNIALIVITHD
                     LSVLAQVTERVAVMYAGRIVEEGAVSDLLITPRHPYTRALVGAFPIVGDPASRGVPAG
                     LPGDPPHPEARPSGCAFHPRCPAAFEPCDSIDPVLERHGQRLAACHLVAREEAR"
     gene            4568..5599
                     /locus_tag="VN178_02070"
     CDS             4568..5599
                     /locus_tag="VN178_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS79782.1"
                     /translation="MKEGTPGHAAELEVRDLEVRFAFRRLFGDSGGEVRAVDGVSFDV
                     EPGEVFCIAGESGCGKTTLGRALLGLVRPHSGNISISGLDPARLKGEELRTFRRQVQV
                     VFQDPMGSLNPRQSVYEAVAEGVRIHGLPGDEKEIVARALARAGLRPPERFFLSYPHE
                     LSGGQRQRVVIAAAIALEPRVLIADEPVSMLDASVRGEILDLLVRLKDEVGMTLVLIT
                     HDLAFAWALADRVAIMYLGKIVELGPAQKVLTEPLHPYTKSLLAVTPEVGRRRTHKVL
                     TGEPPDPVLIPPGCRFHPRCPVLRSGEAGSQEPRCLGEVPVLEETVPGHRVACHVAGE
                     SRILTTRRP"
     gene            5796..7028
                     /locus_tag="VN178_02075"
     CDS             5796..7028
                     /locus_tag="VN178_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWS79783.1"
                     /translation="MLESKIDTREELINALTEAAELEHGLLCQYLFAALSIKKHPTEG
                     ATLPQLELLRSWETHILRVAREEMAHLGTVTNLLTAIGGGPQFRRPNFPQPSRYFPEP
                     AKGGIGQVEFVLEAFSARALERFIRFEAPEEELKLTLEAIGPDPIGYDTVGDLYTQIR
                     EAFETLDERELFIGPRAAQDTDDWSANLKIRNVVDRATAVEAINDIIEEGEAAPTKLP
                     TSHWATFVRISEELEEEVKQDPNFDPARPVVSNPLTRQHRDSSPGATIINDPDTLEIA
                     ELFNAVYGTMMLMLMQFYSFAGESTEQREGLRATARQMMSGVVRPLGEILTQLPARDD
                     LPGRTAGPGFEFYTDLRVPGDRRNSWMIIHERLAHEAEGCRRLAVKTGAPQRLAYLQE
                     NLEGLARNTQRFMNQEEL"
     gene            7029..7865
                     /locus_tag="VN178_02080"
     CDS             7029..7865
                     /locus_tag="VN178_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79784.1"
                     /translation="MLTLNFEGWFQCRLATDPDPSDEPRGVSGWTFAVAGEEDLDRII
                     RLQEPISPRSHGPEVGVVVRTVAVDEQRIPDHPLVGGRIQLLDSPKFEGRNGIVAEDA
                     AEGIDPFHLRITAGGVTLRRRDILDPQHPERKMNEVDPTVLKRRQPVVDDPVERAEIA
                     EATGIMDYLGYRSERKKKLETDLQGEGDPVLRAALQKRIADLKESLEKPDIRVGILGA
                     SLRYRFAIRGPAEVSGDREVLGGIVGTSPPWPIEFWAGGWDADALCGYMRGRLQVPFR
                     PL"
     gene            8027..9226
                     /locus_tag="VN178_02085"
     CDS             8027..9226
                     /locus_tag="VN178_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWS79785.1"
                     /translation="MMKPALKGRGEAYHEHIVSLRRHLHQFPEVSGEEHATSETVQEK
                     LDEHGIPFTAGYARTGVLGVVEGGSPGKTVALRADMDALPIQEENDHSFVSKVDGVMH
                     ACGHDAHTAMLVGAGWLLREVREELPGRVLLLFQPAEEASPTGGARRMIKEGVFSEHS
                     PHVIFAQHVWPELPAGQVGVRGGPMTGASDRFKVTIEGQGGHASKPHQTIDAIVVANQ
                     VINSLQTVVSRDVDPLRSAVLTIGKIRAGTRYNAIAQEATLEGTIRTFGSHTKERVKD
                     RFHSIVGGVAESMEASVRIKYWDGYPATVNTPEWAERVRKTAQDLLGPDATPEIEPSL
                     GGEDFGHFLRDYPGSYFRLGTAFSNAPEKRRLHDSRFDIDEAALQLGTEILAQLAVDA
                     LYELENR"
     gene            9477..9707
                     /locus_tag="VN178_02090"
     CDS             9477..9707
                     /locus_tag="VN178_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79786.1"
                     /translation="MGNTHGRAAQQERDRGGGEKGSVILLFALVVSLCAATVAALFVI
                     MWRDGDHIAAAALAFALLPVLVLAIVAWRAVL"
     gene            complement(9802..10281)
                     /locus_tag="VN178_02095"
     CDS             complement(9802..10281)
                     /locus_tag="VN178_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS79787.1"
                     /translation="MSIFPTRILLATDGSREAQLAASTAADIASSTNSELHVLTVAPG
                     NPDPVYHIHEGGLRYETYQQALEAIQGEAQKVLDEQVQEIEGSGGSVKEAYLRVGERR
                     DQAVVHLAEELGAGLIVMGSRGLGGVRRALMGSVSDSVVRHAHCPVMIVRPERERAE"
     gene            10497..11297
                     /locus_tag="VN178_02100"
     CDS             10497..11297
                     /locus_tag="VN178_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWS79788.1"
                     /translation="MPELPELEVFKRYVDSTSLHQNIETVEVKDGRVLGGVSAGELKR
                     GLEGRKFESTRRHGKHLFVELDDGPWLLLHFGMTGDLKYYKDVVEEPTHARVRISFGD
                     GYRLAFDDQRLFGKVDLIEAPDDFIEEHELGLDPLDLDFSAFRERLEGRRGEIKATLM
                     NQRIFAGIGNIYSDEILFQVRLHPRTSVGLLDGDALHDLHGQTQRVLRAAIERGATPQ
                     GLPDSFLLPHRQEGADCPRGNAKIQKTKAAGRTAYYCPVCQPTLAGKA"
     gene            complement(11397..11606)
                     /locus_tag="VN178_02105"
     CDS             complement(11397..11606)
                     /locus_tag="VN178_02105"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HWS79789.1"
                     /translation="MGLGNLGNLDLGQLTQYLPNLDFPATKDEVVSEAQSNNAPQEVI
                     DQLKNSSTDTFNNADEVLETVRGNR"
     gene            complement(11880..12812)
                     /locus_tag="VN178_02110"
     CDS             complement(11880..12812)
                     /locus_tag="VN178_02110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS79790.1"
                     /translation="MVVGCGVIGLTTAICLREAGLDVGIVAAELPPGTTSGVAAAIWY
                     PYKAYPEARVLFWGGRTFEVFEELSDTPESGVLMREGVEISRLPVPDPWWAGAIPGLR
                     RCNEDELPPGYVDGHAFTAPIVEMPIYLAYLMDRFGAAGGSVQRRTLSSLDEVDAGVV
                     VNCVGLGARDLVDDVSMEPIRGQIIRVSNPGIERFVLDEDNPESVTYIVPRSGDCILG
                     GTAEVGAWDLEPDSETAAGILRRCTRLEPRLTGVEVLEHKVGLRPGRPEIRLEREDGP
                     PPRIHNYGHGGSGITLSWGCAEETLRLVQETLGA"
     gene            12972..>13770
                     /locus_tag="VN178_02115"
     CDS             12972..>13770
                     /locus_tag="VN178_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWS79791.1"
                     /translation="MLDSTKNGAGYVAAEVMARAGVRRLYTVPGESFLEILDAAEEHP
                     GLALVSTRHESGASFMAEAEGKLTGRPAVAMATRGVGASNLAIGVHTARQDSTPMLVL
                     LGQVETDFLGREAFQEVDLGAFYAPITKWAATVHRPDRLAEFVARGLRIATSGRPGPV
                     MLALPADVLGETVQDGPQVRVPPPPRSALAPQDVRAVADRLAAARRPVVIAGGGAQGA
                     REALVSFSEAWGAGVYAAFRRQDVFPNEHPNYLGHLTLGTPQEILKAL"
BASE COUNT         2573 a         4296 c         4319 g         2582 t
ORIGIN      
        1 agctcgttct ccgcatcggg agcaccaacg acatcgacgg gttcaacccg ttcaagatcg
       61 tggagattcc ctcctacgag gtgatgagcc tcacctacga ttatctggtg gacttctccc
      121 cgaaggactc gagtccggta ccaggtctgg cggactcctg ggagacctcc gacgacggcc
      181 tcacctggac cttccacctc aacaaggatg caaagtggca cgacggcaag ccggtgacgt
      241 ccgaggacgt ggcttacacc ttcaaccgta tcctggacga ggagcagggc ctgtacatcg
      301 attatgtcag gcagatagag acgatagaga ctcccgacag gcacacggtc gtgttcgaga
      361 cgaaagaccc ctcggtacag atgctatcca tgttggtcta catcctgccc aagcacatct
      421 gggaggacgt gcccgctgac gagacgaaga ccttcaagaa ctcgccagcg atcggctctg
      481 gtccgttcca ggcagtggag tggaaagaag gccagtccgt gcgcctcgag gccaaccccg
      541 actacttccg gagcgcccct cacatcgacg agatcatctt ccagctctac gacaatgccg
      601 acaccatggt acaggcgctc aagaacggtg aggtagatta catacagccg gtccccatca
      661 acctcttcaa gtcgctcgag aaccaggagg gaatagagac caactcggca ccagaccctg
      721 ggttcaccga gctcggcttc aacctctatg aaccctcccc ggaggcgatt gaggagttcg
      781 acgctcccaa gacgagcacc gggaaccccg ccttgctaga cccgcaggtg cgagaggcca
      841 tcaactggtc catcgacgag gaagagctca cccagaagat cctgcagggc gagggaatcc
      901 ccggctccac ggtagtccct ccggcccttg ccaaatacca cctgcggcta gacgagtccg
      961 agatccaggg tttcgacatc gagcgctcca aagatctgct aagagagtcg ggatggaggg
     1021 acaccaacgg cgacgggacc gtggacaagg ccggcgagga gttggagtta cgcctgttcg
     1081 tgcgctcgga ggacaaggac tccgtaacgg cgggccagtt catcgaggac tggttcgggg
     1141 aggcggggat cgggctcgag accaaggcga taagcgacac ggccctcacc gatgccatct
     1201 atgcggccga ctacgacatg ttcatctggg gttggggctc cgatcccgac ccggacttca
     1261 tcctgtcggt gctgacctgc gaccagatca tgggctggtc ggacactttc tggtgcaacg
     1321 atgactactc gcggatgtac caggagcaaa aggagcagct cgacatcgac gagcgagccg
     1381 ccaccatcaa ggagatgcag cgtatcgcct acgaggagaa cccctacgtc atcttctact
     1441 acgacaatca gaccgaggcc tggcgcaccg acaagttcga gggctggacc aagaccccga
     1501 cggctgccga ccccggccag gtagccttca cgttcacgac caagacgtac ctgaacttgc
     1561 aacccctcag ctcccaagat gaggcgggaa gcggtggcgg aggcacctcg ccactcttgt
     1621 acgtgctgct cgcggtcgtc gccgtggcgg tcatcgcggg cgtggtcttc ttcctccggc
     1681 ggggcagcgc ggaggacagg gcctgagccg ggcgcgctat gtagcggtca agctgatcgg
     1741 ggccgtcggc accctgtttt tcgtgctggt cctgaacttc ttcctgttcc gtattctgcc
     1801 cggagatccc gagcgcacgc tcaccaggtt gcagcgcgtc tcgccctcca ccatcgacga
     1861 ggtaaagagg gaatttgggc ttgaccgtcc tctgacggtc cagttcttcg actatcttgg
     1921 ggatactgcg cggggcgagc tcggcatctc ctacgtcttc tcgcgtccgg tatccgaagt
     1981 gatcgggacc gcgctgtggc cgacgatact gctcgtaggc gtggccacag tggcggccac
     2041 ggtggtcggg atgctcatgg gcatctgggg cgcgtggcgc aggggtagcg ccgtggacac
     2101 ctccctactc ggcttcgggc tcgtcaccta cgcgatgccg gagttctggc tcgggatgct
     2161 gctgatcctt gtcttcgccg tcgccctgga ctggttcccg acggcgttca tgaccacacc
     2221 agggctgacg gacgcctccg caggcgtgca gctcgtggac acgcttgagc acctgttttt
     2281 gccggcgctc acgctgggcc tggcgctgct cggggagtac gcgctcatca tgcgctccag
     2341 cgttatcgag gcgatgaacg aggactacgt caccaccgcc agggccaaag gcatgcgcga
     2401 agccctggtg ctcagaaaac acgtcgtccc aaacgccctg ctgccggtcg ttacgctcgc
     2461 tgccctgaac ctgggtttca tagtctccgg ggcgatcacg gtcgagaccg tgttctcctg
     2521 gccaggcctc gggcaactca ccttcagggc catcaacgga ggaccggatt atccgctctt
     2581 gcaaggcctg ttcttgctgt tctcggtggc ggtgatcttc gccaacctgg cggcagacct
     2641 gctgcttggg ttgctcgacc ccaggatcgg tcgggtatga gcgcccagac cacccgcagc
     2701 ggccgtgccg tgcgccgcac gcggcgcatc caggcgttca agacatcagc aagcctgttc
     2761 gcccggaaca agatgggcct tgcaggtcta gtaatactga ccgttttcac attgacggcc
     2821 ctgttcgccg acgtgctcgt tccggaagca cggctctctg tgatcaacgc gcctggcact
     2881 ccgctcgagc cgccctcggc gcagttctgg ctgggtacgg acgagctggg acgatcggtg
     2941 ctggacttgg tgatccaggg gtcccagatc tccttgctcg tgggtttcct ggcgaccgcc
     3001 atctcgatgg tgatcggggc cctggtcggg ataacggccg ggtacttcgg cggctggaca
     3061 gatgtgatcc tcatgcgcct gaccgactgg ttcctcgtca tccctttcct gcccctggcc
     3121 atagtgctcg ctgccatcct gggccggagc ctgttcgtca tcgcgttagt catcggggtc
     3181 accacctggc cggccaccgc ccgcgtggta cgcgcccagg tcctgaccct caaggaacgc
     3241 ccgtacgtgg agcgtgccag ggccctcggc gcgggagacc gccagatcat cggccgccac
     3301 atcctcccca acgtttcccc actcatcttc gccaacacca tcctgatcgt ggccctcgcc
     3361 atctacaccg aggccatact ctcctttcta gggctgggtg accccttcag agtgtcctgg
     3421 ggcagcatcc tggagtcggc cttcacggag ggcgccatct ccctcggcgc ctggtggtac
     3481 ctggttccgc cgggcctgtg catcgtactg gtggtgctcg gcttcaccat gtgcggctac
     3541 gcctttgacg agatactgga cccgagattg cgagacagat gagcgagacc gccgcgcctg
     3601 ccggaaccct gctcgagata cggaacctgc gcgtcttgta cgaggtgcgg ggcgcgaagg
     3661 ccccggccgt ggacggcgtg gacctcacgc tgcggccggg ggaagctctg gggctcgcgg
     3721 gcgagtcggg gtgtggcaag agcacgctcg cgctggcctg tcttggtctg ttaccacgta
     3781 acgcgacggt cgaaggatct atccgctttc aagagacgga ggtgatcggc gcgaagccgg
     3841 gtcggctgcg ggcgttaagg tgggcgggga tctccatggt tttccagggg gccatgaacg
     3901 cgttcaaccc ggtgaagcgg gttggggatc aggtgatcga ggcgatcctg cttcatgagg
     3961 acgtctccag aagcgaggcc cgcagccgaa tgcaggagct cttcgagcac gttgggatcg
     4021 cgccgcgcag ggctggcgaa tacccgcacg agttctccgg cggcatgcgc cagcgggtta
     4081 tgatcgccat ggccctggcg tgcgacccgc cgctggtgat cgccgacgag ccaaccactg
     4141 cgctcgacgt catgattcag gcccagatcc taaacctcct ggcacagctg cgcgaagaga
     4201 gtaacatcgc cctgatcgtc atcacccacg acctctcggt cctcgcccag gtaaccgagc
     4261 gggtggcagt aatgtacgcc ggccgtatag tcgaagaagg tgccgtctct gacctcctca
     4321 tcacgccccg ccacccctac accagggccc tggtcggcgc ctttcccatc gtcggggatc
     4381 ctgcttcccg cggtgtcccc gcggggctgc ctggcgaccc gccccatccc gaagccaggc
     4441 ccagcggctg cgctttccat ccccgttgtc ccgccgcctt cgagccctgc gatagtatag
     4501 acccggttct ggaaaggcat ggccagcgcc tggccgcctg ccacctcgtc gccagggagg
     4561 aggcgcgatg aaggaaggaa cgccgggcca cgctgcggag ctggaggtgc gggacctgga
     4621 ggtccgtttc gccttccggc gtctcttcgg cgattccgga ggtgaggtac gggcggtgga
     4681 cggcgtctcc ttcgacgtag aacccggcga ggtcttctgc atcgccgggg aatccggctg
     4741 cggaaagacc acactcggcc gcgcgctgct cggcctggtc aggccccact cgggaaacat
     4801 ctcgatctcc ggtcttgacc cggcgcgtct aaagggagaa gagctcagga catttcggcg
     4861 ccaggtccag gtggtattcc aggacccgat gggctctctg aacccccgcc agagcgtcta
     4921 cgaagcggtc gcagagggcg tgcgcatcca cgggttgccg ggggatgaga aggagatcgt
     4981 ggcccgcgcc ctggctcggg ctggtctgag accacccgag cgcttcttcc tcagctatcc
     5041 gcacgagtta tccggaggac agcgccaacg ggtggtcatc gcggcggcga tcgcgctcga
     5101 gcccagggtg ctcatcgccg acgagcccgt ctccatgcta gacgcttcgg tgcggggcga
     5161 gatcctggac ctcctcgtcc ggctcaagga cgaggtcggg atgaccctgg tcctcatcac
     5221 ccacgacctc gccttcgcct gggcgctagc cgaccgggtc gcgatcatgt acctgggcaa
     5281 gatcgtcgag ctcggaccgg cacaaaaggt gctcaccgag cccttgcacc cctacacgaa
     5341 atccctgctc gccgtaaccc cggaggtcgg tcgtcgccgg acccataagg tattgaccgg
     5401 cgagcctccg gatccggtcc tcatcccccc tggctgccgc ttccaccccc gctgtccggt
     5461 gctgcgctcg ggagaggcag gctcccaaga accccgctgc ctaggagagg tcccggtcct
     5521 cgaggagacc gtacccggcc atagagtagc ctgccacgtg gccggagaat cgagaatcct
     5581 gacgacgcgg agaccgtgac cgatgtacca gcgaaactcc ctgtgacagg atttggtcag
     5641 aatctccagt tctctcctgt aaatcggcga gattcatcca gaattcatcg ctctctacta
     5701 tggtgctgta agcgagatga ttgccgtcca ggaatcaagt agaccaacga cgcgagtcta
     5761 ccttgtcctc caacaagaac taataggaga acgatgtgct agagagcaag atagacacca
     5821 gagaagaact catcaacgct ctgaccgagg cggccgagct cgaacatggg ctcctttgcc
     5881 agtacctttt cgcagccctt tcgataaaga aacatcctac cgaaggtgct acgctgcccc
     5941 agctggagct cctccggagc tgggagacgc acatactgcg tgtcgcgcga gaagagatgg
     6001 cccacctcgg cacggttacc aacctcctga ccgcaatagg tgggggaccg cagttccgac
     6061 gtcctaattt tccgcagccg tctcgctact tccctgaacc agccaagggt ggcatagggc
     6121 aggtcgagtt cgtactcgaa gccttctcgg ctcgtgctct cgagcgcttc atcaggttcg
     6181 aggcgcccga ggaggagctc aagctaacct tggaggccat tggtccagac cctatcggtt
     6241 acgataccgt gggcgacctg tacacgcaga tccgggaggc gttcgaaacc ctcgacgagc
     6301 gggaactgtt cattgggccg cgtgcggcgc aggacaccga cgactggtcg gcgaacctga
     6361 agatcagaaa cgtcgtggat cgggccaccg ctgtcgaggc gatcaacgac attatcgaag
     6421 aaggagaagc tgctccgacg aaacttccaa cgtctcactg ggcgacgttc gttcgaatca
     6481 gtgaggaact ggaggaggag gtcaagcaag atccaaactt tgatccggcg aggcctgtcg
     6541 tatccaatcc tctaacgcgc caacaccgcg actcttcgcc cggagccacc atcatcaacg
     6601 acccagatac gctcgagatc gcggaattgt tcaatgccgt ttacgggacg atgatgctca
     6661 tgttgatgca attctactcc ttcgccggcg agagcacgga acagcgagag gggcttaggg
     6721 cgacggccag gcagatgatg agcggcgtcg tcaggcccct cggcgagatc ctgacccagt
     6781 tgcctgcgag ggacgatctt cctggaagaa ccgctggacc cgggttcgaa ttctataccg
     6841 acctgagggt gcctggcgac cggaggaatt cgtggatgat aatccatgag cggcttgctc
     6901 atgaggcgga aggatgcagg cgcctcgccg tcaagacagg agcgccgcaa cgcctggctt
     6961 atctgcagga gaacctcgag ggccttgctc gcaacacgca acgcttcatg aaccaggagg
     7021 aactgtagat gctcacgttg aacttcgaag gatggttcca gtgtcgcctc gctaccgatc
     7081 ccgatccgtc cgacgaaccc cgaggggtga gcggctggac gtttgcagtc gcgggcgaag
     7141 aggacctcga ccgcatcatc aggctgcaag aaccgatctc gccccgttcg cacggacctg
     7201 aagtcggtgt cgtggtgcga accgtggcgg tggacgagca acgcatcccc gatcatcctt
     7261 tggttggcgg ccggatccaa ctcctcgaca gcccgaagtt cgaaggacga aacggcatcg
     7321 tagcggagga tgccgcggag ggtatcgatc ctttccactt gaggataacc gctggaggcg
     7381 taaccttgcg ccgcagagat atcctggacc cacaacaccc ggagcgaaaa atgaacgaag
     7441 tcgatcccac cgtcctgaag cggcgccagc cggtcgtgga tgatccggtg gaacgtgccg
     7501 agatcgctga ggctacagga atcatggatt acttgggcta ccgttcggag cgcaagaaga
     7561 aactcgaaac ggacttgcaa ggggagggtg atcctgtatt gcgcgcggcc ctccaaaagc
     7621 gcatcgccga cttgaaggag agcctggaga aaccggatat tcgggtgggc atactgggag
     7681 ccagcctcag atacaggttc gcgatccgcg gccccgcgga agtctctggt gaccgggagg
     7741 tgctgggagg aatcgttggt acttcacctc cctggccgat cgagttctgg gcgggtggat
     7801 gggatgccga tgctttatgc ggatacatgc gtggcagact gcaggtgccg tttcgccccc
     7861 tctaaccaag gtcgatttcg agcatccgct ccaggacaat ctcgtgtccg tactcctgct
     7921 ttgcctccct cgcggcaaat ctgtccgtgt gtcgctctgg cgacgtggtg tgcggaaggt
     7981 cacgccttcc gcgtaaagct tcgggcgata gaatcaggtg atggcgatga tgaaaccagc
     8041 gttgaagggt agaggcgaag cgtaccacga acatatcgtc tccctgcggc gccatctcca
     8101 tcagttccct gaggtcagtg gggaggaaca cgctacctca gagactgtgc aagagaagct
     8161 agacgagcac ggtatcccct ttaccgcagg ctacgccagg acgggcgtgc ttggcgtggt
     8221 ggaagggggc agcccgggca agactgtggc cctgagggcg gacatggacg ctttgcccat
     8281 acaggaggag aacgaccact ccttcgtctc caaggtggac ggggtgatgc acgcctgtgg
     8341 acacgacgcc catactgcga tgctggtagg ggctggatgg ttgctacgtg aggtaaggga
     8401 ggaacttcct ggtcgtgtac tcctcttgtt ccagccggcg gaggaggctt cacccaccgg
     8461 tggagcacgg cgcatgataa aggagggcgt gttcagcgag catagccccc acgtcatctt
     8521 cgcgcagcac gtgtggcccg agctgccagc agggcaggtc ggcgtacgcg gaggtccgat
     8581 gacgggtgcc tccgaccgtt tcaaggtgac gatcgaaggt cagggcgggc acgcgagcaa
     8641 accccaccag acgatcgacg ccatagtggt ggccaaccag gtcatcaata gcctgcagac
     8701 ggttgtgagt agggacgtcg atcccctccg ctccgcggta ctgactatag ggaagatccg
     8761 ggccgggacg cgttacaacg ctatagccca ggaagcgacg ctcgagggta cgatccgcac
     8821 gttcggatcc cataccaaag agagggtcaa ggaccgcttc cattcgatag tgggcggtgt
     8881 cgcagagtcg atggaagctt cagtccggat caagtactgg gacggttacc cggcgaccgt
     8941 gaatactccc gaatgggccg agcgcgtccg caagacggcg caggatctgc taggacccga
     9001 tgcgacgcct gagatagagc cttccctggg gggcgaggat ttcggacact tcctgcgaga
     9061 ttaccctggg tcctacttca gactcggaac ggcgttctcg aacgctccag agaagaggcg
     9121 actgcacgat tcgcgcttcg acatagacga ggcggcgctc cagctaggca cagaaatcct
     9181 cgcccaactg gccgtcgatg ccctctacga gctcgagaac cggtagagtg gcggtggcac
     9241 ccgagtagcc ctgacagcgt cgtgcataag ggttgaaccc ttatgcacga cgctgtcagg
     9301 gctcttgatc tgtacctcta ttcccaactt cggcagtcct gtcaatcgtt gcatccgcgg
     9361 gaatcttgtc tggttgggaa ctcaaggtcc cctggggaag ttggctggcc ccgtagcgca
     9421 accccttttg ctgacgatca catgggacgg ggctcgcacc gacggaatgt gctacggtgg
     9481 gaaacacgca tggtcgcgcc gcgcagcaag aacgagatcg aggtggcggg gagaaaggca
     9541 gcgtgatcct cctgttcgcc ctcgtcgtct ccctgtgtgc cgctaccgtt gcggccctct
     9601 tcgtaatcat gtggcgcgat ggggaccata tagcggccgc agcgttggct ttcgcactcc
     9661 tgcctgtgct cgtacttgcc atagtggcct ggcgagcggt cctgtgaagg aacggaacag
     9721 gagacctttg cggatcgcgc ctccttctag ggggtgaatt acgagcgaaa ggtaccggtg
     9781 tcccctagtg ggcgtctcga cttactctgc acgttctctc tccgggcgta cgatcatcac
     9841 agggcagtgg gcgtggcgaa cgacggagtc ggagacactt cccatcagcg cccgcctgac
     9901 gccgccgaga cccctgctcc ccatcactat aagtcctgcg cccagctcct cagccaggtg
     9961 gacgactgcc tgatcgcgac gttctccgac cctaaggtag gcttctttga cactacctcc
    10021 ggacccttcg atctcttgca cctgttcgtc gagcaccttc tgggcttctc cctgtatcgc
    10081 ttcgagcgct tgttgatagg tttcgtagcg caaaccgcct tcgtgtatgt ggtagacggg
    10141 atcaggattc cctggcgcta cggtgaggac gtgcaattct gagtttgtgg aactggcaat
    10201 gtcggccgcg gtgcttgcgg ccagctgtgc ctccctggag ccgtcggtgg ctagcaggat
    10261 ccgggttggg aagatactca tcggttcgtc tccttgctcg ctacattcct acgcctcgat
    10321 aatgccatgt accgcggcgc gatgcacccg catggggatc ttagctgcca gtccgcagct
    10381 cgccatggac gatcgagaac tcgaaaatcc gtgatatggt ggtggagcag ggtgcttgcg
    10441 gtccacgtcg ctgagagcat ggaagtagtc ccgagatacc acaaggtgag gtcgttatgc
    10501 ccgagcttcc agaattggag gtcttcaaac gatacgttga ctccacgtcc ctgcaccaga
    10561 acatcgagac ggtcgaggtg aaggacggga gagtcctcgg cggcgtttct gccggcgaac
    10621 tcaagcgtgg tctcgagggc aggaagttcg agtctacgcg gcgtcacggc aagcacctgt
    10681 tcgtggaact ggacgacgga ccgtggctgc tgttgcactt cgggatgacc ggcgacctga
    10741 aatactacaa ggacgtcgtc gaagagccga cccatgccag ggtcaggatc tctttcggag
    10801 acggttaccg tctggcgttc gacgaccagc gcttgttcgg caaggtggac ctgatcgagg
    10861 ccccagatga ctttatcgag gagcacgagc tggggctgga cccactggat ctggacttct
    10921 ccgccttccg ggagcggctc gaagggagga ggggggagat caaggctacg ctgatgaatc
    10981 agcgaatctt cgccgggatc ggcaacatct attccgacga gatcctcttc caggtccgcc
    11041 tccatcccag aacaagcgtc gggctacttg atggagatgc cctccacgat ctccacgggc
    11101 aaacccagcg tgtgctgcgc gcggcgattg aacgcggggc aactccccaa ggcttgcccg
    11161 actctttctt gctcccccac cggcaggagg gagcggattg tcctcggggc aacgcaaaga
    11221 tccagaagac gaaggcggcg ggccgcaccg cctactactg tccagtctgc cagccaacgc
    11281 ttgcaggcaa agcctgatgc ttctttgagc ggtgcccctg tagtttcgac aaggaaagac
    11341 ctggacccag ggtctcccct gggtccaggt tataggcaaa gaatctcgcc cgttctctac
    11401 cggttacccc gcaccgtctc cagcacctca tcggcgttgt tgaatgtgtc ggtgctcgag
    11461 ttctttagct ggtcgatcac ttcttgcggg gcgttgttgc tttgtgcctc cgagacgacc
    11521 tcatccttgg tggccggaaa gtcgaggttc ggcaggtact gcgtgagctg tcccaggtct
    11581 agattgccca gattgcccag gcccatgttc gtgttcctcc catctcactc tctgtcaact
    11641 ccaagaagct ttctaccact ttgccctcag gcaaatcaac atctctaccg tctgagaact
    11701 tgcaaattgg tctagaacct gtttgcaagc tagtcggcac tctccggctt ttagcctccg
    11761 cttgtcagcc cggcggctgc gccgccaggt cggcaagtca gccaggactt tcgcgtgcct
    11821 gctgaagtgc tgacaagtcg cctggaacag gcgacgtgct gacatgctga catgctgctc
    11881 tacgcgccga gggtttcttg cacgagccgg agggtctctt cggcgcagcc ccaggagagg
    11941 gtgatgcctg atcccccgtg gccgtagttg tggatgcgcg ggggcgggcc gtcctcccgt
    12001 tccaggcgta tctcgggccg gcccggcctg agcccgactt tgtgctccag cacctctacg
    12061 cccgtcaggc gcggctccag gcgcgtgcag cggcgcagga tgccggccgc ggtctccgag
    12121 tcgggttcga ggtcccacgc gcccacttcg gcagtaccgc caaggatgca gtcacccgag
    12181 cggggaacga tgtacgtgac gctctccgga ttatcttcgt ccagcacgaa acgctctatg
    12241 ccggggttgc tcacgcgtat gatctggccc cggatcggct ccatagaaac gtcgtctacc
    12301 aggtccctgg ctccgagccc gacgcagttg acgacaacgc ctgcgtctac ctcgtcgagc
    12361 gaggagaggg tgcgcctctg cacgcttccc ccggcggcgc cgaagcgatc catgaggtag
    12421 gcgaggtaga tgggcatctc gactatgggc gcggtgaaag cgtggccgtc tacgtagccg
    12481 ggcggtagtt cgtcctcatt gcatcggcgg agaccgggta tcgccccggc ccaccaggga
    12541 tcggggaccg gtaggcgcga gatctccacc ccctcacgca tgaggacgcc gctctcggga
    12601 gtatcggaga gttcttcgaa gacctcgaag gtcctgccgc cccaaaagag cacgcgggct
    12661 tcggggtagg ctttgtacgg gtaccagata gcagcggcca caccggacgt ggtgcccggc
    12721 ggcaattcgg cggccacgat ccccacgtcc aggccggcct cccgcaggca gatcgccgtc
    12781 gtcagtccga tcacaccgca tcccacgacc acagcatccg cgccggccag aattttctcc
    12841 tctcctcgtc gggcacacta tatgccctgg cggatccttc cgcgacccac ctggggagcg
    12901 agcgtgtagg atgtacggga tgtcagggat gtggtcccgg tgggccgtga tcttccgagg
    12961 acggtggact aatgttggat agcactaaga acggggccgg gtacgtcgcg gccgaggtta
    13021 tggcgcgcgc cggagtaagg cgcctgtaca ccgtgcccgg cgagagcttt ctcgagattc
    13081 tggacgccgc cgaggagcac cccggcctcg ctctagtctc gacgcgccac gagtccggcg
    13141 cctcctttat ggccgaggcg gagggcaagc tgacggggag accggcggtc gcgatggcca
    13201 cgaggggcgt gggcgcatcc aacctcgcca tcggggtaca cacggcgagg caggactcga
    13261 cgccgatgct cgtcttgctc gggcaagtcg agacggactt cctagggaga gaagcctttc
    13321 aggaagtgga tctcggcgcc ttctacgccc cgatcacgaa gtgggccgca acggttcacc
    13381 gccccgaccg cctcgccgag ttcgtcgcca ggggcctgag gatcgccact tctggccgtc
    13441 cgggtcccgt catgctcgcg ctgccggcgg atgtactcgg tgagacggtc caggatggcc
    13501 cgcaggtccg cgtgccgccc ccgccccgct ctgccctcgc gccgcaggac gtcagggccg
    13561 tagcagaccg actcgccgcc gcacggcgtc ccgtcgtgat cgcgggcggc ggggcgcaag
    13621 gcgcccgaga ggccctggtc tcgttttcgg aggcgtgggg tgccggcgta tacgcggctt
    13681 tccgcaggca ggacgtgttc cccaacgagc atccgaacta cctggggcat ctcacgctcg
    13741 gtacgccgca ggagatcctg aaggccctaa
//
LOCUS       DATMVF010000046         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_466677, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000046 DATMVF010000000
VERSION     DATMVF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466677"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..616
                     /locus_tag="VN178_02120"
     CDS             80..616
                     /locus_tag="VN178_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79792.1"
                     /translation="MDKDKHAGSEAKSRASLALVAMLIVALLAAGTWATAGVAGDKRV
                     APDIPGTGQTGATNSTPEEGDLPAARPHPRGLPRRRARPHGRPQEDREPDPLVTETDG
                     RQAPPRAGLISVIGAGLRSLVHPRSPKTAVEEPAGSEGGRGAGQDSAATRRFPMDAGI
                     TRGFTLPLGGTGDQSRRR"
     gene            complement(1629..2207)
                     /locus_tag="VN178_02125"
     CDS             complement(1629..2207)
                     /locus_tag="VN178_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS79793.1"
                     /translation="MPELLVDFITSLDGYGAAEGWPGFWGLEGPEYLAWLEDQPEADY
                     TVLMGANTYRLMSGLADEGEPGTEDLARLSKVVFSSSLSEPLSWPNTRLLSEDPVEAV
                     RVMKAGGDTSMRTIGSLTLCRFLLKAGLVDRFRVVVFPVITGKTGRDRIYDGYPDVAL
                     EMVSSRTFDGRLQLLEYVPRVLAGPPGATASD"
BASE COUNT          480 a          780 c          799 g          448 t
ORIGIN      
        1 taacggccgc cctttacgct gccctcatga ggcgtgtagg caacaggaca tcaacgacaa
       61 ggaaggagaa acacagcaca tggataagga caagcacgcg ggaagcgagg cgaagagcag
      121 agcgtctctg gcgctcgtgg cgatgctgat cgtggcgctc ctggcagcag gaacctgggc
      181 aaccgcggga gttgccggag acaagagggt cgccccggat attccaggca caggacaaac
      241 cggagctacc aactctacac ccgaggaggg agacctccct gcggcccgtc cccatcctcg
      301 cggccttcct cggcgccggg cgcgcccgca cgggcgaccc caagaggacc gcgagccgga
      361 cccgctcgtc accgagacgg acggtcgcca ggcgccgccg agggcaggtc tgatctctgt
      421 gataggcgcg ggtttacgta gcctggtgca tcccaggtcg cccaagactg cggtcgaaga
      481 gcccgccggg tccgaggggg gacgtggagc ggggcaagac agcgccgcga cgcggcgttt
      541 ccctatggat gctggcatca ccagggggtt cacgctgccg ctcggcggga ccggggatca
      601 aagccggagg agatgacgtc tctgtcgaga gcgagacata gtgctctgtc tctaagcacg
      661 cgcagggagg ccacgagacc ttgctcgtgg gcggtgatct tctggctcac aggcttactt
      721 cggtaagcag ccagtcaaag ccttggggag tggagcctgc atgagatacg cgacgggaca
      781 tcaacaaaag gacagagcac tgccgccaga tctttcacgt tttacctgca aacggacgga
      841 agagcggaga gggtgggatt cgaacccacg agacgactta acaccgccta cgcgatttcc
      901 aatctcagaa gggctcctta ccaggttcgt gcgctgcgtc ccgatctatc ccattttgtc
      961 cgtttagtct ggcatattgc ggcatttgtt gaggtgtcat ataccctggc gtccattgcg
     1021 tgcgctcccg tactgtcccg gttgcagtac ggcttgggat cggatgcact atagcgttcg
     1081 cggcggtagt gcctctctgt tacgttcgtc aaccgctcag agtaaaaaca tcctcctgta
     1141 cgcttcaaga tcttgctcgc gaagaacgga gctcggcgag tagaactggc tacgacctag
     1201 agaaggagat gcggtgcaga gccgatcgcc ccgatcccct atgagagagg gtcaggaggg
     1261 cgtacggagg tcgtggcggc ggagggcctt cggtggctga acttccgtta atcggcctac
     1321 tccgaagttg gacttccgct ttacgcagtt ctcggaagtt ccacataact gcgctcctat
     1381 gctgcaggtg cccaccgagc aagcctcgac gtaatgtctg ggacgtatgg cgggaccgag
     1441 cgtggccggt gaagcgagac actggcgaca aggttccagc cgtcggcccg gtcgatcggc
     1501 tcgccgatgc ctggcgcgta ggcgggtgag ctccttctcc ttcgccagca gctccctgcg
     1561 ggcggcgagc cactcctcgg gcgacaccac ctcgggcagg tacatgcctg cggtcatctc
     1621 ggctccagtc agtcggacgc ggtggcgccc gggggcccgg ccagcaccct gggcacgtac
     1681 tccagcagtt gcagccggcc gtcgaaggtg cgactgctca ccatctccag agccacatcg
     1741 gggtagccgt cgtagatccg gtcacgacct gtcttcccgg tgataaccgg gaagaccacg
     1801 acccggaagc ggtccaccag accggccttc aacaggaagc gacacagggt gaggctgcct
     1861 atggtgcgca tggacgtgtc accgccagcc ttcatcaccc ggacggcttc gacgggatcc
     1921 tcactgagga gcctggtgtt cggccacgag agcggctccg acaggctgga ggagaagacg
     1981 accttcgaca gtcgggccag gtcctcggtc ccgggctcgc cctcgtcggc cagcccggac
     2041 atgagccggt aggtgttggc ccccatcagg acggtatagt ccgcctccgg ctgatcttcc
     2101 agccaggcga ggtactccgg tccttccagg ccccagaacc ccggccagcc ttcggcggcc
     2161 ccgtagccat cgagtgaggt gatgaagtcg acgagcagtt ccggcatcct cggctccttt
     2221 ctatctcacg tgcttcatgt cagtagtgta cggtacatgc ccgatgctca gtagtggcga
     2281 tcatcgagtg ttcggcataa cgaatgtcac tcgagcccac cgcacctcgc cccgggccgg
     2341 ccttcacggc ggacgaaggc aggcccataa ccggtcggtc attgctggtc ccgctaaacg
     2401 tcccgcactt cacagcgagg tctccaccga gcattcagta tggtttgagg ttgctcggcg
     2461 ccttagctgc ggtgcctagt ctaaagaact cggcccgagg ctttaag
//
LOCUS       DATMVF010000047         5441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_282599, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000047 DATMVF010000000
VERSION     DATMVF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282599"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VN178_02130"
     CDS             <1..1323
                     /locus_tag="VN178_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HWS79794.1"
                     /translation="RRGKLQRAREGKVMAGRRVKYGFKLNEARDGLLVDEDKMSNVRR
                     IFRMVGTEGQSMNAVYRAFEREQIPTPGGGKRWDRAFFRLAILDDAYSPHTSREVRKL
                     VSPEVAARLEESKLYGISWYNRLRVRTRQVSEPYAGASNGRRYRNEVRIKERPKEEWI
                     AVPVPYAGIPREVVDAARAAIKDNRRSPNAGRRFWELSGGILRCATCGHTTVSHSVRA
                     HSGTHYHHYYYCRNHHKHGDAECAHRKNHRASDIEGRVWDLISGLLKDPERLRAGLDE
                     MVEAERAGMRGDPEAETLTWLARISTLDSKRSRYQDMAAEGHITFDELGAKLRELEGQ
                     RTTAEEELDNLQLRRARLEDLERDRETLLREYAGMLPERLDELTGEERHQIYRMLRLQ
                     VFVYPNGDLEVRGVLSEPREAVCTLMDTRSSTPGVTGPYSNPSGVTGL"
     gene            1242..1841
                     /locus_tag="VN178_02135"
     CDS             1242..1841
                     /locus_tag="VN178_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWS79795.1"
                     /translation="MYTDGYTVVYPRRNRALFQSVRRYGALISEYYLGEAPLAWRFPA
                     RNRIIAGLAGTVVVVEAPEHSGALITARHALDAGRDVWAVPGPPGVRECRGSNGLLAD
                     GAGVLWDIPEFVDAVAREHRVPVAGETSGGDQPLPSGLEDTEAAVLCGVGFEPRGVDA
                     VAKQCDVPMPEVLTALALLELKGFVSRDGVGSYVRRAVP"
     gene            1838..2671
                     /locus_tag="VN178_02140"
     CDS             1838..2671
                     /locus_tag="VN178_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HWS79796.1"
                     /translation="MRQRTLRSLWSHYLRVRKRLAEREMTGVERRMAEREARGLRDRL
                     VVNYSPLVKYVAGRISGRIAGPLDQEDVVASGLFGLLNAVETYDPERRTKFESYAISK
                     IRWSILDELRKVDPLSRRTRMRAHQIERTRDELVQKLGRAPTESELVGRLGISVTEHR
                     AFLEQYARAQVRSLETRLEYQGGPGAWLHDLIGDHSATDPESAAEKAEVRARLVRAIE
                     GLGEQERVVTTFYYYEGLTLKEIGRILDLTEGRISQILHSALVRLRQHLAEEIRFSER
                     T"
     gene            2858..4021
                     /gene="rpsB"
                     /locus_tag="VN178_02145"
     CDS             2858..4021
                     /gene="rpsB"
                     /locus_tag="VN178_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWS79797.1"
                     /translation="MQSTTERVGIRELLEAGVHFGHQAKRWNPKMKRYIFAQRAGVHI
                     IDLDQTLTLLERALSFVRGVAEANREVLFVGTKKQAQIPLAEEAIRSGQPFVAERYIG
                     GMLTNFQTILPRIQYFKDLAVRVDETPEEERTGKDWFALNREYQKLRRNFAGITEMER
                     LPGGVFVIDPKREELLVKEANRLKIPVLALTDTNCDPEVIDYVIPGNDDAIRSISLIS
                     RLVADAIIAGRGEEVQIEERPVPPVIEDSQVEAEVLQTAEVTTEAPNGTSSTPPADEP
                     EAPAEPEAIPEADAAEEAVVTVESEVEEEPEAEGVETEVVDAETVETETVEAEAEDTE
                     AVEAEVIEPEAVDSEAVEPEAATEPEAEEIETEEAGPVEEAPDEKIEETEEKQ"
     gene            4024..4611
                     /gene="tsf"
                     /locus_tag="VN178_02150"
     CDS             4024..4611
                     /gene="tsf"
                     /locus_tag="VN178_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWS79798.1"
                     /translation="MSSTIEKIKQLREETAAGMMDVKRALEESEGDVEGARKVLRERG
                     QALAAKKSGRAATEGLIEAYVHFNGRVGVLVEVNCETDFVARTPEFKEFARNVALHVA
                     SMRPMCVSPEDIPSGALEEERQVVEKQVAEMGKPENISQQIVEGRMRKWVAEQALLTQ
                     PYVKETEKTVGDLLQETIQKVGENVVIRRFVHYEL"
     gene            4621..5424
                     /gene="pyrH"
                     /locus_tag="VN178_02155"
     CDS             4621..5424
                     /gene="pyrH"
                     /locus_tag="VN178_02155"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWS79799.1"
                     /translation="MDESAKPRVTSDGAALPQLGPIKRVVLKLSGESLAGNGGGYGID
                     PDSLDSTARGVLEAVEAGAELAVVVGGGNIFRGSHVADELGIEGATADYMAMLGTVIN
                     ALALQAALEGRGIPTRVQSAIEIQEVAEPYIRRRAIRHLEKGRVVIFASGTGNPFFTT
                     DTGAALRALEINADALLMAKNRVDGVYDKDPRRYPDAVRLPRLNYMDLLSDDLSVMDH
                     TAATLCGGENLPIVVFDILKEGNLRRILQGEQVGSLVWGTANGRGGRDV"
BASE COUNT         1119 a         1604 c         1878 g          840 t
ORIGIN      
        1 cgcaggggca agctgcagcg cgcccgtgag ggcaaagtca tggccggtcg gcgcgtgaag
       61 tatggcttca agctcaacga agcacgcgat gggctgctgg tggacgagga caagatgagc
      121 aacgtgcggc gcatcttccg catggtcggt accgaggggc aatccatgaa cgctgtctat
      181 cgagcgttcg agcgagagca aatacccacg cccggaggag ggaagcgctg ggaccgtgcg
      241 ttcttccggc tggcgattct ggatgatgcc tacagccccc acacctcccg ggaggtaagg
      301 aagctggtat cgcctgaggt ggcggcgcgt cttgaggagt ccaaactcta cggcatctcg
      361 tggtacaacc ggctaagggt ccgcactcgt caggtatccg agccttacgc aggagcatcg
      421 aacgggcggc gctaccgcaa tgaggtgcgc atcaaggaga ggcctaagga ggagtggata
      481 gccgttccag tcccttacgc gggcataccc cgcgaggtgg tggatgcggc aagggcggcc
      541 atcaaggaca accgacggtc gccgaacgcc gggaggcgct tctgggaact ctcgggcggc
      601 atcctgcgct gcgcaacatg cggccacacg acggtcagcc acagcgtccg ggcgcactca
      661 ggcacccact accaccacta ctactactgc cgcaaccacc acaagcacgg cgacgccgag
      721 tgcgcgcacc ggaagaacca ccgagcctcg gacatcgagg ggcgagtatg ggatctcatc
      781 tcgggcctgc tgaaagaccc ggagcgccta cgcgcaggac tggatgagat ggtagaggcc
      841 gaacgcgccg ggatgcgtgg cgatccagag gcggaaaccc ttacctggct cgccaggatc
      901 agcacgctag acagcaagcg ctcccggtat caggatatgg cggcggaggg tcacatcacc
      961 ttcgacgagc tgggcgcgaa gctccgggag ctggagggac agcgcactac ggcagaggag
     1021 gagctagata acctccagtt gcgccgtgcc cgccttgagg acctggagcg cgacagggaa
     1081 accctcctaa gggagtacgc gggcatgttg ccagagcgcc tggatgagct gaccggcgaa
     1141 gagcgccacc agatctaccg gatgctccgc ttacaagtgt tcgtctaccc taacggcgac
     1201 ctcgaagtgc gaggggtgct tagcgaacct agggaagccg tttgtacact gatggataca
     1261 cggtcgtcta ccccaggcgt aacagggccc tattccaatc cgtcaggcgt tacggggctc
     1321 tgatctccga gtactacttg ggcgaggcgc ccctggcatg gagattcccg gcgagaaacc
     1381 gcatcatagc cgggcttgcc ggtaccgtgg tggtcgtgga ggcgccggag cacagcggcg
     1441 ccctcataac ggcccgccac gccctcgacg ccggacgcga tgtatgggcc gtgcccggtc
     1501 ctccgggcgt acgggagtgc aggggctcga acgggctgct cgccgacggt gcgggagtac
     1561 tgtgggacat accagagttc gtcgacgccg tagcacggga gcacagggta cctgtggcgg
     1621 gtgagacatc cggaggcgac cagcctttgc cttccggtct cgaggatacg gaggctgctg
     1681 tgctctgcgg ggtcggcttc gaaccgaggg gagtcgacgc ggtcgcgaag caatgtgacg
     1741 tacctatgcc cgaggtcctt acggcgttag ccctactcga gctgaagggg tttgtgagcc
     1801 gtgatggtgt tggatcttac gtgaggagag ccgtcccgtg aggcagcgta ccctccggtc
     1861 gctgtggtcg cactatctcc gggtccgcaa gcgcctggcc gaacgggaga tgacgggcgt
     1921 cgagcgccgg atggccgaac gtgaggccag gggacttagg gaccggctcg tggtcaacta
     1981 ctccccgctc gtgaaatacg tcgccggcag gatctccggt cgcattgccg gacccctcga
     2041 tcaggaggat gtcgtcgcgt cggggctctt cgggctattg aacgccgtcg agacctacga
     2101 tcccgagcgc cgcaccaagt tcgagtccta tgcgatctcc aagattcgct ggtccatact
     2161 cgatgagctg aggaaggtgg atccgctctc tcgccggact cgtatgaggg cgcaccagat
     2221 cgagcgtacc agggacgagc tcgtgcagaa gctgggccgc gcgccgacgg agtcagagtt
     2281 ggtcgggcgc ctggggatca gcgtcaccga gcatcgggcg ttcctcgaac agtacgcgag
     2341 ggcccaggtg agatccctcg aaacccggct ggagtaccag ggcggccccg gcgcctggct
     2401 gcacgacctc atcggggacc actcggcgac cgaccccgag tcggccgcgg agaaggccga
     2461 agtacgcgcg cggctggtca gggctataga gggcctcggc gaacaagagc gggtggttac
     2521 cacgttctac tattacgagg gcctcacgct gaaggagatc ggcaggatcc tcgacctgac
     2581 cgagggccgc atctcacaga tcctgcacag tgcgctcgtg cggctcaggc aacacctcgc
     2641 cgaggagatt aggttctccg agaggacgta gaagcatcca cccccttgtg ctaacatctg
     2701 cggggtcgga cgagtctatg cttccaggct cgtctgcgac tacgcacgcc cgccgcaggg
     2761 tcctccggac ccgggtggtg gtccgccggg agatccgagg gcggattgcg aggcgcgggc
     2821 ggaggatacc aaccacctta agagagaggt agagactttg cagagcacaa ccgagcgcgt
     2881 tggaattcga gagctgctcg aggccggcgt ccacttcgga caccaggcca agcggtggaa
     2941 ccccaagatg aagcgctaca tcttcgccca gcgggccggg gtccatatta tcgatctgga
     3001 tcagacgctg acgctgttgg agcgggccct gagctttgtg cgcggagtcg ccgaggccaa
     3061 ccgcgaggtc ctgttcgtag ggacaaagaa gcaggcccag atcccgctcg ccgaggaggc
     3121 cataaggagt gggcaaccct ttgtcgcgga gcgctacatc ggcggcatgc tcaccaactt
     3181 ccagacgatc cttccgcgca tccagtactt caaggatctg gccgtccggg tcgacgagac
     3241 tcccgaggag gagaggacgg gcaaggactg gttcgccctg aacagggagt accagaagct
     3301 gaggcgcaac ttcgcgggca taacggagat ggagcgtctc cctggcggtg tgttcgtcat
     3361 cgaccccaag cgcgaggagc tcctggtcaa agaggccaac aggctcaaga tccccgtact
     3421 cgccctcacc gacaccaact gcgaccccga ggtcatagac tacgtgatcc cgggcaacga
     3481 cgacgcgata cgttcgatca gcctgatctc caggctcgtc gccgacgcga tcatcgccgg
     3541 caggggcgag gaagtgcaga tcgaagaacg acccgtgccg ccggtgatcg aagattccca
     3601 ggtggaggcc gaggtgctgc agacggcgga ggtcaccacc gaggcgccaa acggcaccag
     3661 ttccacacca ccagcggacg aaccagaagc cccggcagaa ccggaagcca taccagaagc
     3721 ggatgctgcg gaggaggccg tagtcacggt cgagtccgag gtcgaagagg agccggaggc
     3781 cgagggcgtc gagaccgagg tcgtcgatgc tgagaccgtc gagactgaga ccgtcgaggc
     3841 tgaagccgag gatacagagg cggtagaggc tgaggtcatc gaacctgagg ccgtcgattc
     3901 ggaggccgtc gagcccgagg ccgcaacaga gccagaggcc gaagagatcg agacagaaga
     3961 ggccgggcct gtcgaagagg cgccggacga gaagatagaa gagaccgagg agaagcagta
     4021 actttgtcta gtacgataga gaagatcaag cagctccggg aggagactgc cgctgggatg
     4081 atggacgtga agcgggcgct ggaggagtcc gaaggggacg tcgaaggcgc caggaaggtg
     4141 cttagggaga ggggccaggc cctcgccgcc aagaagagcg gaagggctgc gaccgagggg
     4201 ctgatcgagg cttacgtgca cttcaacggg cgggtagggg tgctcgtgga ggtcaactgc
     4261 gagacggatt tcgtggctcg cacgcccgag ttcaaggagt tcgcccgcaa cgtggcgcta
     4321 cacgtagcct ccatgcggcc gatgtgcgtc tcgcccgaag acatcccttc cggcgcactg
     4381 gaagaagagc ggcaggtagt cgagaagcag gtggcggaga tgggcaagcc cgagaacatc
     4441 tcgcagcaga tagtcgaggg caggatgagg aaatgggtcg cggagcaggc cctcctaacg
     4501 cagccctacg tcaaggagac ggaaaagacg gtcggcgacc tcttgcagga gacgatccag
     4561 aaggtcggcg agaacgtcgt gatccggcgc ttcgtccact acgagctcta gtagccttcc
     4621 ttggatgagt ccgccaagcc cagggtcact tcagacgggg ccgcgcttcc acagctcggt
     4681 cccatcaagc gtgttgtcct gaagctttcg ggggagagcc tcgccggcaa tggcggcggc
     4741 tacgggatcg accctgatag cctcgattcg acggcgaggg gcgtgctgga ggccgtggag
     4801 gccggggccg agctggccgt cgtagtgggc ggcggcaaca tcttccgggg ttcgcacgtc
     4861 gctgacgagc tcggaataga aggggccacg gccgactaca tggcgatgct cggcaccgtt
     4921 atcaacgcct tggccttgca ggcagcgctc gaaggacgcg gcatacccac cagggtccaa
     4981 tccgcgatcg agatccagga ggtcgccgag ccctacatcc gcaggcgtgc tatcagacac
     5041 ctggagaagg gccgcgtcgt gatcttcgcc tccgggacgg gcaatccttt cttcaccacc
     5101 gacacggggg cggcgctcag ggcgctcgag atcaacgccg acgccctgct catggccaag
     5161 aacagggtgg acggggtcta tgacaaggac ccgaggcggt atcccgacgc cgtgcgattg
     5221 ccgcggctca actacatgga cctgctctcc gacgacctgt ccgtcatgga ccacacggcg
     5281 gccacgttgt gcggtggcga gaacctgcct atagtcgttt tcgatatact caaagagggc
     5341 aacctgcgcc gtatcttgca gggagaacag gtggggtcgc tggtgtgggg caccgcgaac
     5401 ggaagaggag gacgagatgt ctgacgtcat agatctgtcg g
//
LOCUS       DATMVF010000048         7264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_327936, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000048 DATMVF010000000
VERSION     DATMVF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327936"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..784)
                     /gene="kduD"
                     /locus_tag="VN178_02160"
     CDS             complement(14..784)
                     /gene="kduD"
                     /locus_tag="VN178_02160"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HWS79800.1"
                     /translation="MGVLDDFSLDGKVALVTGAARGLGQGIALGLAEAGADVAALDIV
                     SVDGTGDMIRNEDRKFHAMNVDLLECDAKLASEVVEGCVSELGRLDILVNNAGIIRRA
                     PALEFGEEDWEAVIKVNLSAAFYLSQAAARHFVAEDKGGRIINTASVLSYEGGVIVPS
                     YSASKAGIANLTRALANEWAPLGINVNAIAPSYFTTELTSALREDVERSEALLARLPA
                     GRFGEPEDLKGAAVFLASDAADYVHGSILPVDGGWLAR"
     gene            complement(784..1269)
                     /locus_tag="VN178_02165"
     CDS             complement(784..1269)
                     /locus_tag="VN178_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HWS79801.1"
                     /translation="MTQEQTEQIISDLGLPDQAIQSDELPWVPQGDRVWFKPLRFDLA
                     TGRWINVLKVQGSGRVNRHRHTGGQVMGFCIKGSWHYLERDWVARPGTLVYEPPGDIH
                     TLVVDEGVEEMQTLFILEGTVQYIDDDDNLIYQDDVFSKLERYLRFCDEQGIEPQDLR
                     Y"
     gene            complement(1308..1760)
                     /locus_tag="VN178_02170"
     CDS             complement(1308..1760)
                     /locus_tag="VN178_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS79802.1"
                     /translation="MRARGYTHVSVSATDLEESVRFYMDFFGMEEVPSPDFSRPVRWL
                     RVGGLQLHLFLDEDPAPQRHHFALDVDDFEAAYGRAEELGARDSGHYSTVRELPDGAV
                     QMYVRDPAGNLVEINWPDVTTLDREVIPEIQKIGGDPEAVLYFEPPER"
     gene            complement(1773..3221)
                     /locus_tag="VN178_02175"
     CDS             complement(1773..3221)
                     /locus_tag="VN178_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWS79803.1"
                     /translation="MIESQIEALNFIGGEWVPADSGETSEIVNPSNPSEVLGTTPKSD
                     KAETERAIEAAAEALPDWKDTLPAARGAVLMEAANIIESREDELARLMALEAGKPMKE
                     ARMEVGRAASIFRYYGSEGWRMPGIAPPSARPGVSHSSVREPLGVVGLITPWNFPLAI
                     PTWKMAPALICGNTIVMKPASNASLNAAALVRILAEAGLPDGVLNMVTGPGGAVGDAL
                     VTDPRVKGISFTGSTSVGLGIQGRAVGKKVQLELGGKNPYVVMEDADLSDAAAKVSFG
                     AFGYAGEKCTASSRAIVVEEVYDEFIEELKSATEAMKVGDPLEEDTDIGPVVNKSQYD
                     SILEALETAKSEGRVVLEGGATGSEDDGYYISPSIVADVDNRSETAQEEIFGPVLAVI
                     KASDFDEAVDLANDTRYGLTAGIATRSLRYAHEFMQRSETGLVNVNLPTAGLEFQVPF
                     GGNKESGVGGREQGPAALDFYSAWKTVSIMPL"
     gene            complement(3289..4101)
                     /locus_tag="VN178_02180"
     CDS             complement(3289..4101)
                     /locus_tag="VN178_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWS79804.1"
                     /translation="MYLSRHQSAAGPRWALEGRYLPAEFTLEQLLEIPGTDVRSFLED
                     LGSEGPSTDPLLPPIEPAHEVWASGVTYLQSREARELESTDADAYQKIYDAVRPELFF
                     KALGWRVVGHGDRVRIREDSHWNVPEPELVLVVNSGMEIVGYTPGNDVSSRDIEGENT
                     LYLPQAKIYDGACSLGPGIVLSGPDAMRDLHITMSISRGDTLVFEDETDTSQMKRSPE
                     ELAAYLGKELAFPGGVFLMTGTGIVPEEDFTLESGDLVGISVGDLLLENEVA"
     gene            4214..5116
                     /locus_tag="VN178_02185"
     CDS             4214..5116
                     /locus_tag="VN178_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS79805.1"
                     /translation="MEILPGIHRIESDLGERFMCQYLLLGEERTVLIDTGLAGTPEEV
                     IVPYLGEIGLGIEHVDEVIISHADVDHCGGNRALKEMKPSLRFSCGEADRAWVESTER
                     IMAEIYLWSEPYGFGPDEETKDWIRTELGGDSPVDMGLRGGETLRLGPDWRVEFLHLP
                     GHTPGHLGIWDAKNDAAIIIDAALEKGIYDREGNLLQPPRYFDATAYQKTIRKLHSMG
                     PGHLLTSHYQPMRDEEALDFLDRSLDFTLQVQRVVGEGLKHGTTGLWNLTKHADAELG
                     PYPAAMVELGAGVRTHLGFLGPIG"
     gene            complement(5113..5955)
                     /locus_tag="VN178_02190"
     CDS             complement(5113..5955)
                     /locus_tag="VN178_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS79806.1"
                     /translation="MVEIDLIGRIGALEVGRRSLAFWGLGQVGVAIKGPGGVIYVDPY
                     LTDSDGAGGSLPRTFPPPLSSAKVTNASAVLLTHAHIDHTDPDTVLPLSEASPAARFV
                     ASFASSDTLVEAGLDGDRILVPQVGEPVEIAGARVTAIPSSHTELEHDPERGYPYLGY
                     VIEWNGVTVYHAGDTVVYNGLIETLSAWDIDVAFVPINGRDYFRTQRGIIGNTDFRET
                     AELTETLDIGLIVPTHYDLIEGNTVDPGHFVSHLYGLNPMRPQKLLRPAELLYFVKDP
                     DDQG"
     gene            6077..7027
                     /locus_tag="VN178_02195"
     CDS             6077..7027
                     /locus_tag="VN178_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HWS79807.1"
                     /translation="MAENLDVITVGETMVLGVPPRPGRLRHAASLELKIGGAESNLAI
                     AISRLGLSAGWAGYLGDDEPGQIVLDRIRAEGVDTSRVRRIENRPTGLYLREQIGTDV
                     RVYYYRRDSAASTMQREAFDPDYFSGARFVHLTGITPALSEGCRAFVLWVAGEVRESG
                     ARLSFDVNYRSKLWGTKQAREFVEEILPDVDLLLAGDEEARALWGRDDEGLVRELARS
                     GPKEVVIKKGSAGSLALVEGEILDHPASEVAEVDPVGAGDAFAAGYLAGHLWDLPAEE
                     RLRVANAMGALSVATLGDYEGLPDEDELWAFLEGEESLGR"
BASE COUNT         1266 a         2307 c         2352 g         1339 t
ORIGIN      
        1 ctcgatgtag tctctagcgt gccaaccagc cgccgtctac gggcaggatc gagccgtgta
       61 cgtagtccgc ggcgtccgag gcgaggaaga cggcggcgcc cttgaggtcc tctggttcgc
      121 cgaagcgccc ggcggggagg cgggcgagca aggcctcgga cctctcgacg tcctcgcgta
      181 gcgcgcttgt cagctcggtg gtgaagtagc taggggcgat ggcgttgacg ttgatcccca
      241 gaggcgccca ctcgttggcg agggcgcggg taaggttggc gatgcccgct ttggacgcgg
      301 agtaggaggg tacgatcacg ccgccctcgt aggagaggac cgaggctgtg ttgatgatcc
      361 tgcctccctt gtcctccgcg acgaagtgcc tggcggcggc ctgggacagg tagaaggcgg
      421 cgctcaggtt gaccttaatg acggcttccc aatcctcctc gccgaactcc agcgccggtg
      481 cccggcggat gatgccggca ttgttgacga gtatgtccag acgaccgagt tccgagacgc
      541 acccttcgac gacttcggag gcgagcttcg cgtcacactc caatagatct acgttcatgg
      601 cgtggaattt acggtcctcg ttccggatca tatctccggt cccatctacg gacactatat
      661 ccagggccgc cacgtctgcg ccggcttcgg cgaggcctag agcgatgccc tgaccgaggc
      721 cgcgcgccgc cccggttacc agggcgactt tgccgtcgag gctgaagtcg tcgagtacgc
      781 ccactagtag cggaggtcct ggggttcgat gccctgctcg tcgcagaacc tcaggtagcg
      841 ttccagcttg gagaagacgt cgtcctggta gatcaaattg tcgtcatcgt ctatgtactg
      901 gacggtccct tcgaggatga agagggtctg catctcctcg acgccctcgt cgacgacgag
      961 cgtgtggatg tcgcccggcg gctcgtagac gagcgtgccc ggcctcgcga cccagtcgcg
     1021 ctccaggtag tgccaggagc cctttatgca gaagcccatc acctggccgc ccgtgtggcg
     1081 gtggcggttg accctgcctg agccctgtac tttgaggacg ttgatccaac gtcccgtcgc
     1141 aaggtcgaac ctcaagggct tgaaccagac cctatcgccc tgcggcaccc agggcaactc
     1201 gtcgctctga atggcctggt cggggagacc gagatccgag atgatctgct ctgtctgctc
     1261 ttgcgtcatg gacttctcct cctgttcgtg gcttgacgtc ctgctgctca gcgttccggc
     1321 ggctcgaagt agaggacagc ctcggggtcg ccgccgatct tctggatctc cggtatgacc
     1381 tcgcggtcga gcgtagtcac gtcgggccag ttgatctcga cgaggttgcc cgcagggtcc
     1441 cgcacataca tctggacggc cccgtctggc agctccctga cggtagagta gtgtccgctg
     1501 tccctcgcgc cgagttcctc ggcccttccg taggcggcct cgaagtcgtc gacgtcgagc
     1561 gcgaagtgat gccgctgcgg ggcggggtct tcgtccagga agaggtggag ttgcagacca
     1621 ccgacccgca gccagcgcac gggcctggag aagtcgggtg aggggacctc ctccatcccg
     1681 aagaagtcca tgtagaaacg gaccgactcc tccaggtcgg tggcgctgac gctgacgtgg
     1741 gtgtaccctc tcgccctcac gctgacgccc ccctacaacg gcatgatgga gacggtcttc
     1801 cacgccgagt agaagtcaag ggccgccggc ccctgctccc gcccgccgac gcccgactcc
     1861 ttgttgccgc cgaaaggcac ctggaactcc aggccagcag tcggcaggtt gacgttcacc
     1921 agccccgtct ccgagcgttg catgaactcg tgggcgtagc gcagagacct ggtcgctatc
     1981 cccgcggtca gaccgtagcg ggtatcgttg gctaggtcga cggcttcgtc gaagtcgctg
     2041 gcctttatga cggcgaggac ggggccgaag atctcctcct gcgctgtctc agaacggttg
     2101 tccacgtcgg ccacgatgga cggggagatg tagtagccat cgtcctcaga gccggttgcc
     2161 ccgccttcta gtaccacgcg cccctcgctc ttcgccgtct ccagggcttc gaggatactg
     2221 tcgtactggc ttttgttgac cacgggtcct atatccgtgt cctcttcgag cgggtcgccg
     2281 accttcatgg cctcggtcgc gctcttgagc tcctcgatga actcgtcgta gacctcttcg
     2341 acgacgatgg cccggctcga ggccgtgcac ttctcgccgg cgtatccgaa ggcgccgaag
     2401 gagaccttgg ccgccgcgtc cgagaggtcg gcgtcctcca tcacgacgta agggttcttt
     2461 ccaccaagct cgagctgaac cttcttaccc acggccctgc cctggatgcc gaggcccacg
     2521 ctcgtcgagc ccgtgaacga gatgcccttg acgcgcgggt cggtaacgag ggcgtcgcct
     2581 acggctccgc ctggtccggt caccatgttc aggacaccgt cgggcaggcc tgcctccgcg
     2641 agtatacgca ccagtgcggc cgcgttcagg gacgcgttgc tggccggctt cattacgatg
     2701 gtattgccgc agatcagggc cggcgccatc ttccacgtcg gtatggcgag agggaagttc
     2761 cacggcgtaa tcagtccgac gacccctagg ggctccctga cggaggaatg gctcacgccg
     2821 ggccttgctg agggcggggc tatgcccggc atgcgccagc cttcggagcc gtagtagcgg
     2881 aagatgctcg ccgcgcgtcc cacctccatg cgggcttcct tcataggttt gcccgcctcc
     2941 agcgccatga gccgcgccag ttcgtcctcg cgcgactcga taatgttcgc ggcctccatc
     3001 aggacggcgc cgcgggcagc ggggagggtg tccttccaat cgggcagggc ttcggccgcg
     3061 gcttcgatcg cccgctccgt ctccgccttg tccgatttgg gggtggtccc gaggacttcg
     3121 gaggggttcg atgggttcac tatctccgag gtctcgcccg agtctgcagg gacccactcg
     3181 ccaccgataa agttcagggc ttctatctgt gattcgatca agatactcct cctgtaaaaa
     3241 cgcctgccta ctctctctac ccgatcctag cccattctat aggccggcct acgccacctc
     3301 gttctcgagc aagagatctc cgaccgagat cccgaccaga tcccctgact ccagcgtgaa
     3361 atcttcttct ggcactatcc ccgttcccgt catcaggaag accccgccag ggaaggcgag
     3421 ttccttgccc agatacgccg caagctcctc gggtgagcgc ttcatctgcg atgtgtctgt
     3481 ctcgtcctcg aagacgaggg tgtcaccacg gctaatgctc atcgtgatgt ggaggtcgcg
     3541 catcgcgtca gggccggaga gcacgatgcc tggtcctagt gagcaggcgc catcgtagat
     3601 cttggcctgt ggaagataca gtgtgttctc tccctcgata tcccgcgagg aaacatcgtt
     3661 gcccggggtg tagcccacga tctccatccc gctgttgacg acgagtacaa gctcgggctc
     3721 gggtacgttc caatggctgt cttcgcggat gcgcaccctg tccccgtgtc ccacgacccg
     3781 ccaccccagc gccttgaaga agagctcggg ccgcacggcg tcgtagattt tctggtaggc
     3841 atcggcgtcc gtcgactcca actcacgggc ctccctgctc tgcaggtatg tcaccccgct
     3901 ggcccagacc tcgtgcgccg gctcgatcgg cggcaggagc gggtctgtgg acggcccctc
     3961 ggaacccaga tcctccagga agcttcgaac gtccgtaccc ggtatctcga gaagttgctc
     4021 cagcgtaaat tctgcgggaa ggtagcgtcc ttccagcgcc caacgtgggc ctgcagcgct
     4081 ctggtgacgg gacagataca tagtggtcgg gcctcctctc ctcgcaaaca caacctaaca
     4141 cgattccggc gccgttgcgc ggggcacggg tggtggtaat gtggtgccac cagcgtcttc
     4201 cgaggaggat gggatggaga tactgccggg gatacaccgc atcgagtcag acctcgggga
     4261 acggttcatg tgccaatacc tgctcctcgg agaggagaga acggtcctca tcgacacggg
     4321 cctcgccggg acgcccgaag aggtcatcgt cccctacctg ggagagatcg gcctcggcat
     4381 cgagcacgtg gacgaggtga tcataagcca cgctgacgtc gaccactgcg gcggcaaccg
     4441 tgccctcaaa gagatgaaac cttccctgcg gttctcatgc ggcgaggccg accgggcatg
     4501 ggtggagagt accgagcgga taatggcgga gatctacctc tggtccgagc cctacggttt
     4561 cggccccgac gaggagacca aagactggat ccgcacggag ctcggtggcg actccccggt
     4621 tgacatggga ctgcgcggcg gcgagacctt gcggttggga cccgactggc gggtcgagtt
     4681 tttgcacctc ccgggccaca cgccgggcca tctgggcatc tgggatgcca agaacgacgc
     4741 ggccatcatc atagacgcag cgctcgagaa gggtatctac gaccgcgaag gcaacctcct
     4801 gcagccgccg cgctacttcg acgccaccgc ctaccagaag accatccgca aactccactc
     4861 catgggtcct ggccatctcc ttacgtccca ctaccagccc atgcgggatg aggaagccct
     4921 tgacttcctc gaccgctcac tcgatttcac cctgcaagtc caacgggtgg tcggcgaagg
     4981 gttgaagcac gggacgacag gcctttggaa tctcacgaag cacgccgacg cggagctcgg
     5041 cccgtacccg gcggccatgg tcgaactagg ggcgggggtc aggacgcatc tcgggtttct
     5101 cggccccatc ggttagccct gatcgtctgg atccttgacg aagtagagta gctcagcggg
     5161 acgcagcagc ttctgcggac gcatcgggtt caggccgtag aggtggctga cgaaatgccc
     5221 ggggtcgacg gtgttgcctt cgatcaggtc gtagtgcgtg ggcacgatca gaccgatgtc
     5281 cagggtctcg gtgagttcgg cggtctcgcg gaagtcggtg ttcccgataa tgcctctctg
     5341 cgttctgaag tagtcgcggc cgtttatcgg cacgaaggcg acgtctatgt cccaggcgga
     5401 gagggtttcg atcaggccgt tgtagaccac ggtgtcgccg gcgtggtaga cggtgacacc
     5461 gttccactcg atcacatacc ccagataagg gtagccgcgc tcggggtcgt gctccaactc
     5521 cgtgtgggac gaagggatgg ccgtgaccct ggcacccgcg atctccaccg gctccccgac
     5581 ctggggcacg aggatacgat ccccgtccag ccccgcctcg acgagcgtgt cgctgctggc
     5641 gaaagacgcg acgaagcgcg ccgcgggcga agcctcggag agcggcagca ccgtgtccgg
     5701 gtcggtatgg tcgatgtggg cgtgcgtcag taggaccgcc gacgcgtttg tgaccttggc
     5761 ggaggagagc ggtggaggga aggtgcgcgg caggctgcct cccgcaccgt ccgagtcggt
     5821 gaggtagggg tccacgtaaa tgacgccgcc aggacctttg atcgccaccc ccacctgccc
     5881 gagcccccag aaagcgagcg accttcgtcc tacctccagc gcgccgatcc tacctatcag
     5941 gtcgatctcg accacctccc cttctcgaac cttcgtcgcc gaaaccctcc tcgagccggc
     6001 gcaggtggcg accagattag cacaagcaga gtcgctctcc ggaagagata atgtccgtac
     6061 aaaggaggga ccaaggttgg cagagaacct ggacgtgata acggttggcg agacgatggt
     6121 cctcggagtt ccgccgcggc ccggacgact gcgccacgcc gctagcctgg agctcaagat
     6181 cgggggcgcc gagtcgaacc tcgccatcgc tatctcccgc ctcggtctct ccgccgggtg
     6241 ggccggatac ctcggcgacg acgagccggg ccaaatcgtc ctcgaccgga tccgcgccga
     6301 aggggtggac acctcaaggg tgcgaaggat agagaaccgt ccgacgggac tgtatctgcg
     6361 ggagcagatc ggcacggacg tccgggtcta ttactacaga cgggactcag ccgcatcgac
     6421 gatgcaaaga gaagccttcg acccggatta cttctcgggc gcaaggttcg tgcacctgac
     6481 gggcattacg ccggccctct cggaaggatg ccgcgccttc gtcctgtggg ttgccgggga
     6541 ggtccgcgag tcgggcgcgc gcctcagctt cgacgtcaac taccgctcga agctgtgggg
     6601 gacgaagcag gcgcgggagt tcgtcgagga gatcctgccc gacgtcgacc tgcttctcgc
     6661 cggggacgaa gaggcgaggg cgctctgggg ccgcgacgac gaagggctcg tgagagagct
     6721 cgcaaggagt ggcccgaaag aggtggtgat caagaagggc agcgcgggaa gcctggcgct
     6781 ggtcgaggga gagatcctgg atcaccccgc ctccgaggtg gcggaggtgg accctgtggg
     6841 agccggcgac gctttcgccg ccggatacct ggccggccat ctgtgggacc tgcctgcaga
     6901 agagcgcctg cgcgtggcga acgcgatggg cgccctcagc gtcgcaacct tgggagacta
     6961 cgaaggcctg cccgacgagg acgagctatg ggccttcctc gaaggtgaag agtctctggg
     7021 gagatgatac ggaactgtga aggtggaacc gtgaaatggg agagggttgg ctgacgcaca
     7081 actccagatt ccttattccc agatcaaaag cgggaggtgc agggatgcaa gagatagaag
     7141 gcgtctacgt cgccaacgtg acgccgttca gggatgacgg taccctcgcg gtcgacgagg
     7201 aagcatacct cgaacacgtc gcctggctcg cggagatggg cgtgcggggc gtggtgcctt
     7261 tcgg
//
LOCUS       DATMVF010000049        12881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_374766, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000049 DATMVF010000000
VERSION     DATMVF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12881
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374766"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..843
                     /locus_tag="VN178_02200"
     CDS             73..843
                     /locus_tag="VN178_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79808.1"
                     /translation="MTEQAPQDEKREEKHKPEPRDTAYWARYAETLRVAGVAEGATNI
                     NVEGRRAVGPLQGFGQMWQKTYRVRLVGAEVTPVEVIKTWKENYKDFWPEGNIFYAPL
                     AGITPGEVALISGSLPGGVKLSTGVMVLYADDESFSLMTPEGHPFSGWITFSSFEEEG
                     TTVAQVQVLMRANDPLYEMGLRMGGHKMENEMWRKTLENLAAHFGVHEPVEMNLVCVD
                     PKLQWSHYRNIWHNAGIRSALYTITAPLRWRRSRARQD"
     gene            879..2300
                     /locus_tag="VN178_02205"
     CDS             879..2300
                     /locus_tag="VN178_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS79809.1"
                     /translation="MAGTYDAVVVGSGPNGLAAAIELAQSGRRVAVLEGRSTIGGGMR
                     SAEITLPGFVHDLGSAIHPLGYASPFFRSLPLGEHGLEWIHPPAPLAHPFDDGTAAVL
                     ERSIAATAAALGPDGAAYRRLMEPISADAGRIADFFVGRPSLLRHPLALASSGVWSLR
                     DARTLAESVFEGEWARGLFAGNAAHSFLRLDQSPSAMFGLLLGTLGHAFGWPFPRGGS
                     QALADALASYLASLGGEIYTDAVVRSVEDVPRTRAVLFDVTPRQLFEIAGEHFTEGYN
                     SALKRYRYGPGVFKVDFALDGPIPWTAEGCKRAGTVHLGGTLDEVAAGEGDVSRGEHP
                     ERPFILLAQQSLFDRTRAPEGKHTVWAYCHVPNGSTFDMTGRIEAQIERFAPGFGDLV
                     LARSVAGTADLERWNPNLVGGDINGGYMDLGQLFTRPMPRINPYSTPADGLYICSSST
                     PPGGGVHGMCGYLAARVALGYLR"
     gene            2479..3933
                     /locus_tag="VN178_02210"
     CDS             2479..3933
                     /locus_tag="VN178_02210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS79810.1"
                     /translation="MFEVVVIGGGPSGVIAALRARELGATVALIEREHLGGVSTNDGC
                     VPTRVLARAARLMRDAEQFADYGLGGVPPTVDFVRLLNRTEHIVNTVHQKKQLRTHLE
                     AADVRVFDGIGDARFLDDHTLSLGNGSSVQGEKFILCVGGHARRIPLPGGEHALTHSD
                     VWTMKGLPESVVVVGGAATGCQLASVFAAFGSRVTLLEVSPRILAAEDETVSEAVTDA
                     FARRGIEIITGIGGVDKVESENGRLSLFYAKNDETHTLAAQTVMFAVGWPGNVEGLNL
                     EAVGVETRRGGYVQVDDSLRTSAPHIFAAGDITGRMMLVQSATNEGNLAAEQTVLGGE
                     HGYRHTIVPHGGFTDPEYASVGLTESRARSEGHDYAVAVVPYAELDRGVVDGHTEGFC
                     KLLVDRASRHIVGAHIVGEQAVEVVQIVATAMRAGMPVEQLADVEFAYPTFTSIVGIA
                     ARRILRELGLVAVSPRWGIPERTLGTEWEHSDRD"
     gene            complement(4137..7067)
                     /locus_tag="VN178_02215"
     CDS             complement(4137..7067)
                     /locus_tag="VN178_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS79811.1"
                     /translation="MFRTTISLLGPPRIEHDGAPVEVDTRKAIALAAYLAVTEQRHSR
                     DALAGLLWPEYNQSRARAALRRTLSSLGKARAEGWLRADRESVGLDYDAIWVDVSRFH
                     GLLAECKGHDHPESEVCPDCLLPLTEAVALYRDDFMAGFGLRDSVAFDDWQFFQSESL
                     RKELAGVLERLAWGRGALGEWEAAIDHARRWLAMDMLHEPAHRMLMALYAWSDQRAAA
                     LRQYRECVRVLDQELGVAPLEETTLLYRAVQENDLPPRPVLSEHRPPVWREAKASTRS
                     PIQTTSIAPRSPDNPLVGRGPEWEALLGSYRSIEEAGQVVVVEGEAGIGKTRLAEEFV
                     ASVRAAGATAVIARCYAGEKNLAYGPFVEGLSAALGRQDTDRLEGLPAVSVQEAARLL
                     PDLANLSLDSPPAPPLDTPGARSRFFREVVRVLLEILDGPPPGVLFFDDLHWVDDASL
                     DLLAYLVSRLDDKPIYVLLTWRTEEVHEVQRLRELLAQARRSGRATTLTLERLGPASV
                     EELVGHAVPGAGMLGTRLSDETEGLPLFLTEYLTAVEKGELDPRDEAWTLPGGVQDLL
                     KTRLLRVGEAAGQVLAAAAVVGRSFDFDTVRAASGRGEEETLTALEELMSRGLIREVG
                     SPVGGAPAYDFDHDKLRTLVYGETSLARKRLLHRRAAVALAGQARGRETEALAGQIAR
                     HYRLAGQDEEAARYYRLAGDYAQSLHANSDALAHYEEALALGYSDASALHEAIGDLRT
                     RAGEYGAALASYEAAASSAVQGDLSVLEHKIGNVHARRGERDLARSHYESALEALGEP
                     GGEPAGGLARLYADWSLLSHGQDERDEAAGFARRALELAEGTGDTRSLAQAHNMLGIL
                     AGRSGDQEAALWHLEESLDLAETLADSDVKVAALNNLALAREHSGESDEALRLAETAL
                     ALCVASGDRHREAALHNNLADLLHAAGREQESMAHLKRAVELFAEVGERDELRPEIWK
                     LVEW"
     gene            complement(7271..9145)
                     /locus_tag="VN178_02220"
     CDS             complement(7271..9145)
                     /locus_tag="VN178_02220"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWS79812.1"
                     /translation="MTQQINQGTAGAEQWRSLAQQLRVDSIRSSTGAGSGHPTSSMSA
                     ADLIAVLMSKYLSYDFDDPENPNNDHLIFSKGHASPLLYSLYKAAGAISDEELMTFRT
                     FDSRLQGHPTPQIPWVDVATGSLGQGLPIGVGVALAGKYLDELPYRVWVLCGDSEMAE
                     GSMWEAFQHASYYKLDNLIAILDMNRLGQTRETMDGWNGDHYAARAEAFGWNTIQIDG
                     HDPEQIDKAYARALDQTDAPTLIVAKTKKGRGVSFLEDVDGMHGKPVTEDQEQAALEE
                     LGSSDDLLVEVRKPDPGAGENFTGGLTGRLELPAWDVGDEEATRGAYGAALKALGGAR
                     EDVVALDGEVSNSTKSEEFAEEFPGRYFEMFIAEQQMVAAAVGMSVRHRVPFASSFAA
                     FFTRAYDFIRMAAISGANIRLSGSHAGVSIGEDGPSQMALEDLAMMRAVHGSTVLQPC
                     DANQTARLVAEMADTNGIVFLRTLRPKTPVIYDPSEDFPVGGSKIVRSSDEDAVTVVA
                     TGITVHEAIKAADELQRNGLAVRVIDAYSIKPIDAETLRAAAQATEGNVVAVEDHWPE
                     GGLGEAVLSALAGENYPLHFEHLAVRNMPGSGKPEELMDAAGISSKHIADAARKLASS
                     "
     gene            9349..10947
                     /gene="pckA"
                     /locus_tag="VN178_02225"
     CDS             9349..10947
                     /gene="pckA"
                     /locus_tag="VN178_02225"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HWS79813.1"
                     /translation="MDLAVRKMTLGRFGVENVGAVHENLPPARLVEASVRRREGMISG
                     SGALVVRTGKRTGRSPKDRFIVENEITRETVDWGTGNKAFPSDAFGALLNKAAAYIEN
                     LEEIYVVDAYAGADPRYRLNVHVVTEHAWQALFARQLFRRPSKEEIDSFEPDWTVISV
                     PGFLTDPEEDGTESETFVGIDFERKVVLVCGTAYAGEIKKSIFTVLNFVLPTERGVFP
                     MHCSSNAGKEGDVALFFGLSGTGKTTLSADPERDLIGDDEHGWSDSGIFNFEGGCYAK
                     TIDLSPEKEPQIWDAIRFGAVLENVMMDRRTREVDYTDGTLTENTRVAYPLEYIAGAV
                     TEGRGVHPSAVLFLTADAFGVLPPISALTPSQAAYYFLSGYTAKLAGTEAEMESEVEA
                     TFSTCFGAPFLPLPATTYAEMLSERLSKHRTRCYLINTGWSGGPYGVGSRIDIAATRQ
                     MVSSVIGGKLDDAETRKDPFFGLNVPVEVPGVDGSVLDPRGTWDDGDAYDEQARKLAD
                     LFRENFSRFESQVTEEVRKAGPHS"
     gene            11041..11565
                     /locus_tag="VN178_02230"
     CDS             11041..11565
                     /locus_tag="VN178_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79814.1"
                     /translation="MAKTDDLREHEQVLLRLLDEMGRSLARQERLMEELENRHVHMRN
                     SWIQEGADGSFMLPDEQELADELQRELERGRDQELELHEILDVLKRRCELEDRLLDEL
                     ESHLPRDHPDQLPLELVGRFERRDQLLEDIEFESFREKRDRLAQTMQLLPELEGLLFE
                     VVERHLVRLHREGG"
     gene            complement(11577..12074)
                     /locus_tag="VN178_02235"
     CDS             complement(11577..12074)
                     /locus_tag="VN178_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS79815.1"
                     /translation="MQLYLVDGARLRELREQAGLSQEELSLMIGQSGTWAEEMEREGR
                     VHVLEIIVQKLTRIFGVEISDLSTFPDPPAPTVLEEPGLDVEKLRALREEASISQEQL
                     DELAELEPGSIARAESVDPSSEEIHASVDTIWKLAMALEVSPTILTRMRGDLHQIEPR
                     RPLDS"
     gene            complement(12128..12376)
                     /locus_tag="VN178_02240"
     CDS             complement(12128..12376)
                     /locus_tag="VN178_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS79816.1"
                     /translation="MKVDGAMLAAARERVFMSQEELAESIGMNPAVVIRLESASRTEI
                     RENLGEDLMQILFVGRSELTSYPDPPEPTLEVPSDTAE"
     gene            complement(12695..>12881)
                     /locus_tag="VN178_02245"
     CDS             complement(12695..>12881)
                     /locus_tag="VN178_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79817.1"
                     /translation="GGQRVAGELGTQVMGRIPLEPDGTGEPGGSLFEEGTIPALAFEE
                     IAASLAATKVRRRIKVL"
BASE COUNT         2218 a         4143 c         4184 g         2336 t
ORIGIN      
        1 gaggatgccg tgagggcggc gaactcggca tagaggcgtg ccgaaggagg cccaagaagg
       61 gagtgacact cgatgaccga gcaggccccg caagacgaga aacgggaaga gaagcacaag
      121 cccgagcccc gcgacaccgc ctactgggcc cgctacgccg agactctcag ggtcgccggc
      181 gtggccgagg gcgcgacgaa catcaacgtc gagggacgca gggccgtagg tcccctgcag
      241 ggcttcgggc agatgtggca gaagacctac agggtaaggc tcgtcggcgc cgaggttaca
      301 cccgtcgagg tcatcaagac ctggaaagag aactacaagg acttctggcc cgagggaaac
      361 atcttctacg cgccgctggc cggcataacg cccggcgagg tcgccctcat ctccggctcg
      421 ctgccaggcg gcgtcaagct ctccacgggc gtgatggtcc tctacgccga cgacgagtcc
      481 ttctctttga tgacgccaga gggacatcct ttctcgggtt ggatcacctt cagttccttc
      541 gaggaagagg gcactaccgt agcccaggta caggtcctga tgcgagccaa cgatcctctc
      601 tatgagatgg gcctccgcat gggcggccac aagatggaga acgagatgtg gcgcaagacc
      661 ctggagaacc ttgccgccca tttcggcgtc cacgagccgg tcgagatgaa cctcgtctgc
      721 gtcgacccca agctccagtg gagccactac cgtaacatct ggcacaacgc aggcatacgc
      781 tccgccctct acacgatcac ggcccctctc cgctggcgcc gcagccgcgc gagacaggat
      841 tagtcgcgaa ctctagagcc gcaatctacg atcggggcgt ggcggggacc tacgatgcag
      901 tcgtcgtcgg ctcgggcccc aacggcctcg cagcggccat agagcttgcc cagagcggac
      961 ggcgggtggc cgtgctcgag ggcagaagca ctatcggagg gggcatgagg tcggcggaga
     1021 taacgcttcc cggcttcgtc cacgacctcg gctccgcgat acaccctctg ggttatgctt
     1081 cgccgttctt ccgctcgctg ccgctgggag agcacggcct tgaatggatt caccctcccg
     1141 ccccgctcgc ccacccgttc gacgacggta cggcggcggt gctggaacgt tccatcgcgg
     1201 cgacggccgc cgcgctcggc cccgacgggg cggcctaccg ccggctgatg gagccgatct
     1261 ccgccgacgc cggcaggatt gcggacttct tcgtcggcag gccaagcctt ctcaggcatc
     1321 ccctggcgct cgcgtcatcc ggcgtatggt ctctgcggga cgcccgcacc ctggccgaga
     1381 gcgtgttcga aggtgagtgg gcgaggggtc tgttcgccgg caatgcggcg cactcctttt
     1441 tgcggctgga ccagtcgccg agcgccatgt tcgggttgct gctagggacg ctgggccacg
     1501 ccttcggctg gcctttcccc agagggggtt cgcaggcgct cgcggacgcc ctcgcgtcct
     1561 acctggcgtc tttggggggt gagatctaca cggatgcggt ggtacgttcg gtcgaagacg
     1621 tgccacgaac ccgcgcggta ctcttcgacg taacgccgcg ccagctcttc gagatcgccg
     1681 gggagcactt caccgagggt tacaacagcg ccctcaaacg ctaccgctac ggtcccggcg
     1741 tcttcaaggt ggacttcgcg ctcgacgggc ccatcccctg gacggctgag ggttgcaagc
     1801 gggccggcac cgtccacctc ggaggcacgc tcgacgaggt cgcggcgggt gagggcgacg
     1861 tctcgcgcgg ggagcacccc gagaggccgt tcatcctgct cgcccagcag agcctcttcg
     1921 accgtacgcg cgcccccgaa ggcaagcaca ccgtctgggc ctactgccac gtccccaacg
     1981 gctcgacctt cgacatgacc gggaggatcg aggcgcagat agagcgcttt gcccccggtt
     2041 tcggggatct cgttctggcc aggagcgtcg ctggaacggc cgacctcgaa cggtggaacc
     2101 ccaacctcgt cggcggcgac atcaacggcg ggtacatgga tctcgggcag cttttcacca
     2161 ggcccatgcc caggatcaac ccctattcga cccctgcaga cggactctac atctgctctt
     2221 catcgacgcc accgggtggg ggcgtccacg gcatgtgcgg ctacctggct gcccgcgtgg
     2281 ccttggggta cctccggtaa cggggcgggg tacagcggcc gggaggacga taagggtgtt
     2341 ctactctaaa ctgcgcgagg agacggacct cgacaggccg ggtggagagc tggtctcggt
     2401 agaaagggag acctgagcac gtctcgctgt ggtcgagacc gcccggcatg agcgaacccc
     2461 atgaaagcga ggtaagaaat gtttgaggtg gtcgtgatcg gcggtggacc ttccggggtt
     2521 attgcagccc tgcgcgcccg cgagctgggt gccaccgtcg cgctcatcga gcgcgagcat
     2581 ttgggcggcg tcagtacgaa cgatggctgc gtcccgaccc gcgtgctcgc cagggctgcg
     2641 cgtctgatgc gagatgcgga gcagttcgcc gactacgggc tcgggggtgt gccgccgaca
     2701 gtagatttcg ttcgcctgtt gaaccgtacg gagcatatcg taaacacggt ccaccagaag
     2761 aagcagctgc ggacccatct cgaagctgcg gacgtgcgcg tgttcgacgg gatcggcgac
     2821 gcccgctttc tcgacgatca caccctctca ctcggcaacg ggagtagcgt gcaaggcgag
     2881 aaattcatcc tctgcgtggg cggtcacgcg cgtaggatcc ccttgcctgg tggcgagcac
     2941 gccctgaccc acagcgacgt ctggacgatg aaggggctgc cggaatcagt cgtcgtggta
     3001 gggggcgccg cgacgggatg ccaactggcc tctgtgtttg ccgcgttcgg ctcgcgggtg
     3061 acgttgctgg aggtctcgcc gcgcattctg gcggccgagg acgaaacggt atcggaggca
     3121 gtaaccgacg cattcgcgcg ccgggggatc gagatcatta ccggcatcgg cggtgtcgac
     3181 aaggtcgaga gcgagaacgg gcgcctcagc ctgttctatg cgaagaacga cgagacgcac
     3241 acgctcgccg cacagaccgt catgttcgcg gtcggttggc ccggaaacgt ggagggattg
     3301 aacctggagg ccgtgggagt ggagacgagg cgtggcgggt acgtgcaagt ggacgactcc
     3361 ctcaggacca gcgctccgca catcttcgcc gccggggaca taacggggcg tatgatgctg
     3421 gtacagagcg ccaccaacga gggaaatctc gccgccgaac agaccgtgct cggcggggaa
     3481 catggttaca ggcacaccat cgtaccgcac ggcggcttca ccgaccccga gtacgccagc
     3541 gtggggctca ccgaaagtag ggctcgcagc gaagggcacg actacgccgt tgcggtcgtg
     3601 ccttacgcgg aactggatcg cggcgtcgtc gacgggcaca cggagggctt ctgcaagctt
     3661 ctcgtggacc gcgcatcgag gcacatcgtg ggcgcgcaca tcgtgggaga gcaggccgtg
     3721 gaggtggttc agatcgtggc gacggctatg cgggccggga tgcctgtcga acagctggcg
     3781 gacgtggagt tcgcctaccc caccttcaca tccatagtgg ggatcgcggc ccgccgtatc
     3841 ctccgcgaac tgggccttgt ggccgtctcg ccgcggtggg gcatacccga acgtaccctc
     3901 gggacggagt gggaacacag cgatagggat tgaggaaggg ttcacccttc ctcaatccct
     3961 gctggtcagc tagtcagcac gctccagcct gcggctgtcg cttgtcagca tttcagcttt
     4021 ccatgggcat atccttgctg acatgctgag agcgaagccc gcaagggctg agcgtgctga
     4081 caaggcgcga agcgccgtgc tgacaagcgg aggcgaagcc ggagcgtgct gaaatgctac
     4141 cactccacga gtttccagat ctcgggccgc aactcatctc tctcgcctac ttcggcgaag
     4201 agttcgacgg cccgcttgag gtgtgccatc gactcttgtt cgcggcccgc cgcgtggagc
     4261 aggtcggcca ggttgttgtg tagcgcggcc tccctgtgcc tgtcccctga ggccacgcac
     4321 aacgccaggg cggtctcggc gagtctcagg gcttcgtccg attccccgct atgttcgcgg
     4381 gcgagggcca ggttgttgag ggcggcgact ttcacgtccg agtctgcgag cgtctccgcg
     4441 agatccaggc tctcctcgag gtgccacaga gccgcttcct ggtcgccgga tctgccggcc
     4501 aggatgccta gcatgttgtg ggcctgggcc agtgaccgag tatctcccgt gccttcggcc
     4561 agctccagcg cccggcgggc gaagccggcg gcctcgtcac gctcgtcctg gccgtgggag
     4621 agcaggctcc agtccgcgta gaggcgggcg agcccgccag cgggttcgcc tcctggctct
     4681 ccgagcgctt cgagggctga ttcgtagtgg ctcctggcga gatcccgctc ccccctgcgg
     4741 gcgtgaacgt ttccgatctt gtgttcgagt acggagaggt caccctggac ggccgaggac
     4801 gccgcggctt cgtagctcgc gagtgccgcg ccgtactcac cggctcgggt ccttagatct
     4861 cctatcgcct cgtgcagcgc gcttgcgtcg gaatatccga gggcgagcgc ctcttcatag
     4921 tgggcgaggg catcgctgtt ggcgtgcagg ctttgcgcgt agtcccccgc gagtcgatag
     4981 tatcttgcgg cctcttcgtc ctggcctgcc agcctgtagt ggcgggcgat ctggccggcg
     5041 agggcctccg tttcgcgccc ccgtgcctgc cctgcaagcg cgaccgcggc gcggcggtgc
     5101 aggagacgct tgcgggccag gctggtctcc ccgtagacca gggttcgcag cttgtcgtgg
     5161 tcgaagtcgt aggccggggc ccctccgacc gggctaccga cctcccggat caagccccgc
     5221 gacatcaact cttcgagggc cgtcaacgtc tcttcttcgc ccctgccgct cgccgccctt
     5281 actgtgtcga agtcgaacga acgcccgacc acggccgccg cggcgagcac ctgccccgcc
     5341 gcctcaccga ccctgagcag acgggtcttg aggagatcct gcaccccccc tggaagcgtc
     5401 caggcctcgt ccctggggtc caactcgcct ttctcgacgg ccgtcagata ctccgtcaga
     5461 aagagcggca ggccctcggt ctcgtcactg agccgtgtac ccagcatccc cgcgccaggc
     5521 acggcgtgcc cgaccagctc ctcgacggag gccgggccca accgctccaa ggtcagggtc
     5581 gtcgccctcc ccgacctcct ggcctgcgca agcaattctc gcagacgctg tacttcgtgg
     5641 acctcctcgg tgcgccaggt gaggagcacg tagatcggct tgtcgtcgag ccgactcacg
     5701 aggtaggcca acagatccag agaggcgtcg tcgacccagt gcaggtcatc gaaaaacaag
     5761 acaccaggag gagggccgtc gagaatctcg agcaagaccc tcacgacttc cctgaagaag
     5821 cggctccggg cgcccggagt gtcgagcggc ggggccggag gtgaatcaag agagaggttc
     5881 gccaggtcag gcaacagccg ggccgcctcc tgcaccgata cggccggcaa accctccaga
     5941 cggtctgtgt cctgccgacc gagggcggcg gagagtcctt cgacgaaagg accgtaggcc
     6001 aggttcttct cgcccgcgta gcagcgcgcg atgacggcgg ttgcgcctgc ggcgcggacg
     6061 ctggccacga actcctcggc gagcctggtc ttgccgatac ctgcctcgcc ctcgacgacg
     6121 actacttgac ccgcctcctc gatcgaacgg tacgatccta gtagcgcttc ccattctgga
     6181 cctcgcccta ccagggggtt gtcgggcgag cgcggtgcga tgctcgtggt ctggatgggg
     6241 gacctcgtag aagcctttgc ttctctccag acgggcggcc gatgttcgga gaggaccggt
     6301 cgcggtggta gatcgttctc ctggacggcc ctgtagagca gggtcgtctc ctcgagcggg
     6361 gctacgccca actcctggtc caggaccctt acacactccc tgtactggcg aagggcggcg
     6421 gcgcgctggt cggaccacgc gtagagcgcc atgagcatcc tgtgggcggg ctcgtgcagc
     6481 atgtccatgg ccagccagcg tctggcgtgg tctatcgccg cctcccactc tcccagcgcc
     6541 ccgcgacccc acgcgagcct ctccagcaca ccagccagct ccttacgcag gctctcggac
     6601 tggaagaact gccagtcgtc gaaggctacg ctgtcgcgca ggccgaaccc ggccatgaaa
     6661 tcgtcccggt acagcgcaac ggcctccgtc agcggaagca agcaatccgg gcatacctcc
     6721 gattcgggat gatcgtgccc cttgcactcg gcgaggaggc cgtgaaacct ggatacgtcg
     6781 acccagatag cgtcgtagtc gagacctacg ctctccctgt cggcgcgcaa ccaaccctcc
     6841 gcacgggcct tgcccagcga cgagagggtc cgacgcaggg cggcgcgggc gcgactctgg
     6901 ttgtactcgg gccagagcag gccggcgagt gcatcccggg agtgcctctg ctccgtgacg
     6961 gccaggtagg cggcgagggc aatggccttg cgcgtgtcga cctcgaccgg cgcaccgtcg
     7021 tgctcgatgc gcggcggacc gagcagtgat atcgtcgtcc ggaacatgcc cgcttctccc
     7081 aatggctctg cgatgacgat attatgccgc cgctaccaca acatcgcaac ggttttcgca
     7141 aggtgagaag gttcgtaacg ttttatagag ctctcagcga ccagccgtca gctttcagct
     7201 ttttgttggc tgatcgctga gagctgatcg ctcgtcgtac ccagggctgg taagccctgg
     7261 gtacgacgcc ctagctgctc gccagcttgc gcgcggcgtc tgctatgtgc ttcgagctta
     7321 tgcctgctgc gtccataagc tcttctggct tacccgagcc tggcatgttc cttacggcga
     7381 ggtgctcgaa gtggaggggg tagttttccc cggcgagcgc tgacaggacg gcctcgccga
     7441 ggccgccttc cggccagtgg tcctcgaccg cgacgacgtt gccctctgtc gcctgtgctg
     7501 cggcccgcag tgtctcggcg tctatgggtt tgatggagta tgcgtctatg acgcgcacgg
     7561 caaggccgtt cctttgcaac tcgtctgcgg ccttgatggc ttcgtgaacc gtgatgcccg
     7621 ttgcgaccac ggtcaccgcg tcttcatcgg aggagcgcac tatcttgctt ccgcccacgg
     7681 ggaagtcttc cgaggggtcg tagatcacgg gcgtcttcgg acgcagcgtc ctcaggaaga
     7741 cgatgccatt cgtgtcggcc atctcggcga cgagcctggc ggtctggttg gcgtcgcagg
     7801 gctggagcac ggtcgagccg tgcacggccc gcatcatcgc caggtcctcg agggccatct
     7861 gtgaagggcc gtcttcaccg atcgagacgc cagcgtgcga gccggagagg cggatgttgg
     7921 cgccggagat cgcggccatc ctgataaagt cgtaggcgcg ggtgaagaaa gcggcgaaac
     7981 tcgaggcgaa cgggaccctg tggcgcacgc tcatccccac ggccgcagcg accatctgct
     8041 gctcggcgat gaacatctcg aagtagcgtc cgggaaactc ctcggcgaac tcttcggact
     8101 tggtggagtt actgacctcg ccgtcgaggg cgaccacgtc ctccctggcg ccgcccagcg
     8161 ccttgagggc ggcaccgtag gccccgcggg tcgcttcttc gtcccccacg tcccaggccg
     8221 gcagctccag ccggccggtc aggcccccgg tgaagttctc tcccgcgccg gggtcgggtt
     8281 tgcgtacctc gaccagtagg tcgtcgctgc tgccgagttc ctcgagcgcc gcctgctcct
     8341 ggtcttccgt gaccggcttg ccgtgcatgc cgtccacgtc ttccaggaac gagaccccgc
     8401 gtcccttctt ggtcttcgcg acgatcaggg tcggcgcatc tgtctggtcc agcgcacgtg
     8461 cgtaggcctt atcgatctgc tctgggtcgt ggccgtcgat ctggatcgtg ttccagccga
     8521 aggcctcggc gcgggcggcg tagtggtcgc cgttccagcc gtccatcgtc tcgcgggtct
     8581 ggccgagacg gttcatgtcc aggatggcta tgaggttgtc gagcttgtag taggaggcgt
     8641 gttggaaggc ctcccacatc gagccctccg ccatctccga gtcgccgcaa agcacccaga
     8701 cccggtaggg aagttcgtcc aggtacttgc cggccagcgc caccccgacg cctatcggga
     8761 gtccctggcc aagggagccc gtcgccacat cgacccacgg gatctggggt gtggggtgcc
     8821 cttgcaggcg gctgtcgaac gtacggaaag tcatgagctc ttcgtccgag atcgcgcccg
     8881 ccgccttgta tagcgagtag agcagtgggg aggcgtggcc cttggagaag ataagatggt
     8941 cgttgttcgg gttctccggg tcgtcgaagt cgtagctgag gtacttgctc atcagcacgg
     9001 cgatgaggtc ggcggccgac atggacgagg tgggatggcc cgagccggcg ccggtgctgg
     9061 agcggatgct gtcgaccctc aactgctgcg cgagcgagcg ccactgctcg gcacccgcgg
     9121 tcccctggtt gatctgttgc gtcaccacta acctcctggg cagaatcgca cgtagcacag
     9181 atagtacttc actaccccgc gctggcgatt cggaaactgt cctgccgcgc ctggtatcga
     9241 ccatacagac gggggcttac cggctttgct agaatccctg gggcgagagg aaaaagaccc
     9301 tgaccgtccg ttctgcgcgt cgggtttgac tgggtgaagg ggtgaacgat ggatctggct
     9361 gttcgcaaga tgacgctggg acgctttggg gtggagaacg tcggggccgt acacgaaaac
     9421 ctgcctcctg ctcgcctggt agaggcctct gtcaggaggc gggagggcat gatctcgggt
     9481 tctggggccc tggtcgtcag gacgggcaaa cggaccggac gctccccgaa ggaccgcttt
     9541 atcgtcgaga acgagatcac cagggagact gtggactggg gtactggaaa caaggctttc
     9601 ccgagcgacg ctttcggggc gctcctaaac aaggcggccg cctacatcga gaacctcgag
     9661 gagatctacg tggtcgacgc ctacgccggc gcggaccccc gctaccgact gaacgtccac
     9721 gtggtgaccg agcacgcctg gcaggccctc ttcgcgaggc agctctttag acgaccctcg
     9781 aaggaagaga tcgactcatt cgagcctgac tggaccgtga tctcggtccc ggggttcctt
     9841 acagaccctg aggaggacgg aacagagtcc gagaccttcg tcgggataga cttcgagcgc
     9901 aaggtagtac tcgtatgcgg cacggcctac gccggggaga taaagaagag catcttcacg
     9961 gtgctcaact tcgtgttgcc caccgaacgc ggcgtcttcc cgatgcactg ctcgtcgaac
    10021 gctgggaagg aaggcgacgt tgcgctgttc ttcggcctct cggggacggg caagacgact
    10081 ttatccgccg accccgagcg ggatctgatc ggggacgacg agcacggctg gtccgactcg
    10141 gggatcttca acttcgaggg cggctgctac gcgaagacga tagacctttc ccctgagaag
    10201 gaaccccaga tatgggatgc tatccgcttc ggcgccgtgc tcgagaacgt gatgatggat
    10261 aggcgcacac gcgaggtgga ctacaccgac ggcacgctga cggagaacac ccgcgtcgcc
    10321 tacccgttgg agtacatagc gggcgcggtc accgaaggaa ggggggttca tccttcggcc
    10381 gtcctctttc tgaccgccga cgccttcggg gtgttgcccc ccataagcgc cctcacgcct
    10441 tcgcaggcag cctactattt cctctctggc tacaccgcca aactagctgg taccgaggcc
    10501 gagatggagt ccgaggtcga ggcgacattc tccacttgct tcggcgcccc tttccttcct
    10561 ctgccagcta ccacctacgc cgagatgctc tccgagcggc tgagtaagca ccgcacccgc
    10621 tgctacctga taaacaccgg ctggtccggc ggtccatacg gcgtaggtag ccgcatagac
    10681 atcgccgcca cccgccagat ggttagctcc gtgatcgggg gcaagctgga cgacgcggag
    10741 accagaaagg accccttctt cggcctcaac gtgcccgtcg aggtgccggg ggtcgatggc
    10801 agcgtcctcg acccacgtgg gacctgggac gacggcgacg cctacgatga gcaggccagg
    10861 aagctcgccg acctcttccg tgagaacttc tccaggttcg agtcacaggt caccgaggag
    10921 gttcgcaagg caggaccaca ttcctgactg atggccaacc cggggtagaa ggtcgcctcc
    10981 acccggggcg cgcacgttta caatcatggc agggactgag acggcgaatg gaggaggcct
    11041 gtggcaaaaa cggacgatct ccgggagcac gagcaagtcc tgctccgatt gctagacgag
    11101 atggggagaa gtctcgccag gcaggaacgg ctaatggagg agttagagaa ccggcacgtc
    11161 catatgcgga actcctggat acaggagggg gcggatgggt cgttcatgct gccggatgag
    11221 caagagctag ctgacgagtt gcagcgtgaa ctcgaacgtg ggcgcgacca ggagctcgag
    11281 ctacatgaga tcctggatgt actgaaacgc cggtgcgagc tggaagaccg actgctcgat
    11341 gaattggagt cccacctgcc tcgagaccat ccggaccagt taccactaga attggtgggc
    11401 cgtttcgaga ggcgtgacca gctcctggaa gacatagagt tcgaatcttt ccgggagaag
    11461 agagatcggc tcgcgcagac gatgcagctc ctgcctgaac tggagggact gctcttcgag
    11521 gtcgtggaac gccatctcgt tcggctccac cgcgaaggtg gttagggaat cagtcgtcaa
    11581 gagtccaagg ggcgccgggg ttcgatctga tggagatctc ctcgcatcct ggtgaggatc
    11641 gtgggcgaga cttccagggc catggccagc ttccagatcg tgtccaccga ggcgtgaatc
    11701 tcctcagagc tcgggtctac gctctctgcc ctcgctatcg agccgggctc cagctccgcc
    11761 agctcgtcga gttgctcttg ggagatggag gcttcctccc tcaaggcgcg tagcttctcc
    11821 acgtccaggc ccggctcttc gagaaccgtc ggagccgggg ggtcggggaa ggtagacagg
    11881 tccgagatct caaccccgaa gatcctggtg agcttctgga cgatgatctc caggacgtgc
    11941 acccggccct ccctctccat ctcctcggcc caggtgccgc tctggccgat catcaaggag
    12001 agctcctctt gcgagagacc ggcctgctct ctgagctccc gtaaacgagc cccgtctacc
    12061 aggtatagtt gcatctcggt cgttcctttc cggctcgttg aagatttcag ccttcgccta
    12121 ccacggctca ctccgctgtg tcgctcggga cttcgagggt aggctccggc gggtcagggt
    12181 agctggtcaa ctccgacctc ccgacgaaga ggatttgcat gagatcctcg ccgaggttct
    12241 ccctgatctc ggtccggctt gcactctcca atcttatgac tacagcgggg ttcatgccga
    12301 tagattcggc cagttcttcc tgtgacatga aaaccctctc cctcgcagcc gccaacatcg
    12361 ccccgtctac cttcacgacc acacctcctc gctctgtctc cagcgtctgg tccacgatag
    12421 tcgtgacctg aagcgatcgc catacatcgc gctcctggca gacgttgtcc ccgcatcctc
    12481 gttgtgcaat tctacccaat gggcgccgcg aagtcgtgcc ggaagcacag agtctcccgc
    12541 caatgctcag attctgcatg ctccgagtga gcgtgcccgg actttagtgg atgtgtagtt
    12601 cctatcctag gagaggttac aagagcgcgc gccaggtgcc tgaagaagtg gtagccccat
    12661 gcgaagccac ctccttatcg gtggctgacc agccttacaa caccttgatg cgcctgcgga
    12721 ccttcgtggc ggccagggat gcggcgatct cctcgaaggc cagggccggg atcgttccct
    12781 cctcgaagag ggaccctccc ggctcacccg tgccatcggg ctcaagcggg atgcgtccca
    12841 tcacctgagt gcctagctct cctgctaccc tctggcctcc g
//
LOCUS       DATMVF010000050        19213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_374772, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000050 DATMVF010000000
VERSION     DATMVF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19213
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374772"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VN178_02250"
     CDS             complement(<1..1003)
                     /locus_tag="VN178_02250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWS79818.1"
                     /translation="MSVEVETVRTVEVPTRMVHGPGAIGRLGEIVEELGVRRPLLVTD
                     GGVVGAGLAEQALERLTDACVFDGVRPNPDVELIGRTSEVYREEGCDGLVALGGGSSM
                     DTAKGVGVEIVHGGSIADYEYGKTPITRRIPPLVTVPTTAGTGSEVTLWAVITDHGRR
                     IKFNVGGTALIGAHVALIDPALTVGLPTAITAATGMDALSHGIECYTCDYHQPFTDSF
                     ALTAIEYVARWLRSACEDGANMEARTFMAHGATFGGMAYGTESAGAAHAMSQSAGGVH
                     DCPHGALTARVLGPVCEYNAPAAPERYARIAQALGADTRGLEPLEAALVGIEELYRLT
                     "
     gene            complement(1000..2487)
                     /locus_tag="VN178_02255"
     CDS             complement(1000..2487)
                     /locus_tag="VN178_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWS79819.1"
                     /translation="MSTRPNIVVIVSDTFRRDHLGAFGNTYIHTPHLDEFARSSVVFD
                     RHVISSFPTMPARADILTGTFSYTHMGWQPLPHHLPTLPGLLSEAGYLTMGVVDTPFF
                     VRGGFGYDRGFDDFIWVRGQGDDTRPHERSDYRKTWRSESDRLVSRTVTEAEQWLERH
                     YKERFFLYVDTWDPHEPWDAPDYYTSLYRAGYEGRQIYPSYGRWAEAGLTRDEVELAH
                     AAYCGEVTMVDFWVGRLLAKLEVLGLGEDTLVFFISDHGFYFGEHGYFGKAEWINDQD
                     AAVTEDAVVPDWLPESWLLTVGWSPLYRELTNVPLMIRGPGLEPGRRPALTTAPDLAP
                     TILDLVGVERPGSMQGESFGDVLVGEKDEHRPFVVSSWPLYFAAGEFTTAIDSRPRRI
                     ASYMPITVTTPERSLILGGPAYMPELYDLGRDPGEQSNVWESHVEEGAALGERALSFL
                     EREGTPELYLDPRRLALRRFAPDWSAPTLLDTKENQMHNANEEAV"
     gene            3100..3861
                     /locus_tag="VN178_02260"
     CDS             3100..3861
                     /locus_tag="VN178_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWS79820.1"
                     /translation="MLQTVDRALRLLLLFEEVDQEYRVGEMAAMLGVDKSSASRLAAT
                     LANRGFLERAPGSEAFRIGPELERLGVLSVGGSHNLIEVARRPMENLAAETEETVNLA
                     RLEDGKVVNISQVDGAHLVGVGDWTGWKTEPHATANGKVLLAFVENGAEGLRLQAPLE
                     AFTEQTITNSEDLRSELDRVRSEGWASTLGELEDGFNGVAVPVSDALGRCRASLSVSG
                     PVYRMPPALLPEIAVLCKKAAEEISASLGRTRSSA"
     gene            4288..5262
                     /locus_tag="VN178_02265"
     CDS             4288..5262
                     /locus_tag="VN178_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79821.1"
                     /translation="MLSKLFSREPDTRVFFATDIHGSETCWRKFLNSGKHYEANIMIL
                     GGDMTGKALVPIVEEGPGRWHATLLENRREFESEDEVEEFEDSVKRRGYYPFRTDPDE
                     MGELESDEEMRNELFYKEMLGTIERWMRMADEKLEGTDIECFVSPGNDDQFEVDEIIA
                     NAKRVRLAEGKVVEFGDFQMVSTGWSNRTPWDTYREEDEEDLAVRLKKMTDQVTAPPE
                     RTIYNFHCPPYGSGLDDAPELTEDLRPKHGGRSIVPVGSTAVRDAIKEGQPALALHGH
                     IHEAKGNTRIGRTLCINPGSSYEQGQLLGAVVNLDGGKKVKRFVLTSG"
     gene            5274..6881
                     /locus_tag="VN178_02270"
     CDS             5274..6881
                     /locus_tag="VN178_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWS79822.1"
                     /translation="MASNTTAGSGAGDLFQRKATGLLRGWSVLDGFIYAAFAINLMTL
                     GLWIFSFAPFIPEGSLIYAIILSGLYLVFQAVTYASLIAVMPRAGGDYVWMSRVLGGG
                     IGFVLAVTGWWFILWHWVPIYANILNVEVLVPLATILRFDGVANFFGAPGGMFTASMA
                     VAILASILISLGMRTYARIQKFCFYGGVIGLVIMFSLLLFNSNADFVGAFNSHAQELY
                     GAGGNAYQQTLDAGSIETNLGALAFGPTLLLIPMIVFFNLWSNWGATLYGEVRGASDF
                     RKNIYAMGGALIVTSAVAVIMLLLFAKTFGWDFYYAASNAYWSGNGPISAWPYPGLFA
                     AFLFDSAILQFILLALLALWFFGWVGSVFLSSTRVVFATAFDRVLPEWVSKTSRNGVP
                     YYALALMLIPSIPISYLYAFGENFATYTLDATLVIATTFLGSAIAAAVLPWRKPEIYN
                     ASPIARYNVLGIPLVTVAASLFSLFLVFCLFKWLTDDTYGINNGQSLIYMAILYGVAV
                     AVYVIFRLLRRAQGMDLNMVYDEIPEE"
     gene            complement(7008..8390)
                     /locus_tag="VN178_02275"
     CDS             complement(7008..8390)
                     /locus_tag="VN178_02275"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HWS79823.1"
                     /translation="MPQTVTMDKIVAFCKRRGFVYQSSEIYGGIRSSYDYGPLGVEMK
                     RNIKEEWWRRMVHMREDMVGLDSAIIMHPRVWEASGHTLTFNDMLVESRTSKRRYRAD
                     HLIEDATGIDAEGLSPEQLTKIIQEDDRVKDPVDGGRDFAPVRPFNLMFETYMGPVKT
                     PENLAYLRPETAQGIFVNFKNVLQTSRVKVPFGIAQIGKSFRNEITPGNFIFRTREFE
                     QMEMEFFVEPGTDEAWHEYWIEERWNWYTNLGLKVENLRRYEHPQEKLSHYSKRTVDL
                     EYNYASVGWSELEGIANRTDFDLKQHAAYSGENLEYFDQTTGERYVPYVIEPAVGPDR
                     IMLAFLIDAYTEEEVRGEERTVLKLHPRIAPTKAAVFPLSKKEPVSTIARELYDDLKG
                     DYRIFYDDSGSIGRRYRRQDEAGTPFCVTVDFDTIEDKKVTIRDRDTLEQERVPIAAV
                     RDRLKALITS"
     gene            8547..9116
                     /locus_tag="VN178_02280"
     CDS             8547..9116
                     /locus_tag="VN178_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HWS79824.1"
                     /translation="MAGLGVGLASVAGVRAFVPLALLSLFVRLGFIAEPDIFGLQIGW
                     TVVLALSALAAVEIMLDKVRALDPAFGFVMIPIRMVAGALLFAAVYEPGVLFDAGAVP
                     GLVAGGAIAGVVAGLEVILRPPARSSSAGVSTAFLSAMEDLVAVIGGVVGFFLPLVPL
                     LFVGFLLFFFNRIRKRRGRKYGGLRILGD"
     gene            9144..10127
                     /locus_tag="VN178_02285"
     CDS             9144..10127
                     /locus_tag="VN178_02285"
                     /inference="COORDINATES: protein motif:HMM:NF003259.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HWS79825.1"
                     /translation="MDEREITIVKGGRETPFSRGILAQTLAQAGARAELAHQIASEVR
                     AELLAEQVYTVEEEEVLARVRDKLVKKDAFVVGRLDKWRVLRESTEPIVVLIGGATGV
                     GTSTLAADVARRLNIQSVVGTDSIREVLRRAISPDLLPLLHKSSYEIKREDLRVPVEE
                     DEMVLYGFRQQASQVAVGVEAIVDRGLKEGTNLVVEGVHLVPEIILNRYRDHPNVCTL
                     VVYLSDEHVHRSRFYIRALGTAMRRPAEEYISYFQEIRQIHDYIVESSARAGVQAVEN
                     LSIEHTSDAAVEIVANRVSGIADRAGRHASAMLLDGATPRQGIPVTRAKSG"
     gene            10153..10575
                     /locus_tag="VN178_02290"
     CDS             10153..10575
                     /locus_tag="VN178_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWS79826.1"
                     /translation="MRVLDRQRLRTFVLGGAVGAILGVLLAPRSGKELRGSLANRAGE
                     ARERSRETYFDAQEQMRERLAEVREGSRGRPEAVDLDASSPASGEAGAQRPYLRDVSR
                     DVGSEALDEDAERSEELRRKVRETRERLRGPKEGGPER"
     gene            10572..11186
                     /locus_tag="VN178_02295"
     CDS             10572..11186
                     /locus_tag="VN178_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79827.1"
                     /translation="MKPTSFVPMVGEISAEDAGEVPGLGAVISYWTGDRYSAEGWFRE
                     LNDVWGTAGFSVRRGGEVQGFVVYGPPERLARAARFPLGPIDDEAVLLAYVGGDARTR
                     RRLLVRMLRDLRQRGVGRVEAIASDRGVSNHVPTSFLLESGWQPVRRAAYRMSYYTLA
                     HIDLKSAVEVGELARALVGRVKLPGLKSPVPGALARTTGEQRSR"
     gene            complement(11411..11914)
                     /locus_tag="VN178_02300"
     CDS             complement(11411..11914)
                     /locus_tag="VN178_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79828.1"
                     /translation="MRDIVFWLSGATLAFLLTAGAFLILANLATAPSEKRLNPDQARG
                     ESGQAVDLILDRERLASLEPLPDQSLELEVENEGTEQLSDVNMTLSVASENTALSDRR
                     YYRQTVEKIPAGETASVRFEFDLPAREDPVAKPPTENPEPSREILEIRATTPEGDSTV
                     RTVILPP"
     gene            complement(11911..13119)
                     /locus_tag="VN178_02305"
     CDS             complement(11911..13119)
                     /locus_tag="VN178_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79829.1"
                     /translation="MLSRIGPAKRVAVLSVAAVFMAGCTHAEKEVSIAPLVPAAGRLD
                     PGLGPETRALSSGPGYKGSPSWSPGGARIAFTVDGYVVDRPTDSEEPRRWTTRDFTAE
                     DTEWISEDTLMILGAASPTGTEESPSSLYRTRAEGDSLELERIEKEVSAIGTGRGGLI
                     LARGRGASESRLALTRGNGDVRRLYSESIKGRVASLSVSPDGDEAVLTVRPPGDLQTS
                     RLHMFDLRKGRVREITRLDGNREILGTPQWTGQYLYFVAGVKNTPLDRDGSEPLYEIF
                     RVPEEGGAPEPAPGVGEDFVATSIRVSPDGERLAIIGRLSPQAPTNLYVLDPLAEDLT
                     AVTTNEDMEIKTGPDDLAWSPGGQSVVVVARGTPSTEPEVHAAPAVRLLEDFYNLYEI
                     PVGGPEDAPR"
     gene            complement(13109..14983)
                     /locus_tag="VN178_02310"
     CDS             complement(13109..14983)
                     /locus_tag="VN178_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS79830.1"
                     /translation="MGIEKREDRGLPPALNFKARIFVALVGISSVTSLIFGLVLYYFA
                     QDRLVHAENTLLRQRSQTANSGAQDFLEGLRDPEDKTLPPPPDYADELVQAVADPTGL
                     EVLYLGPDMEPLAARDGLGDPLDPREAFGGLDKAELEEIASSPGGEGRLVWSEGRSRY
                     VAVWPLTAYDGTEKGVLIYHVPRDELGDTLAYLRYGILGAILTSILLAGAASLLLTRR
                     VTRPLTETRDAAIRVASGDYAATVPVKSRDELGEVARSFNYMAEEIEHYVGEIQEQKS
                     RLEAVLEASPEAVVATDSGERVTMVNAAAARMLGIRASDRGRRLGETGVPSAVLKCLR
                     EAAANGVAVREVELGEKAFWAYAAQMDRGQPTNAPNGDAGIILAVRDITEHRSLEKAK
                     TAFVSDLSHELRTPLTTIQSAVGLLERARDRLDPLEHRALELADQELKRIRGMVEELM
                     TLAQMDSWKYQLEVGPANMSTIVQTAIESVEAKAQRFGIKIYFDNADEHRCICDVQKL
                     YQVFLNLLDNAIKYSDSGARVDVEIEEDDSTLTVRIRDTGVGIPEEDLNQLFERFYRV
                     DKDRSRATGGSGLGLAISRQIVEMHGGSISVESEVDVGSVFEVRLPKAFLSRSVSHAL
                     "
     gene            complement(14994..15683)
                     /locus_tag="VN178_02315"
     CDS             complement(14994..15683)
                     /locus_tag="VN178_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS79831.1"
                     /translation="MDETILLVDDDAALLEVTSIVLASEGYRVVTAEDGAEALRILGR
                     EGLDLVVLDIMLPRMSGFEVLKKMREHSDVPVVLLTAKSQSVDKVVGLELGADDYITK
                     PFDTKELLARIKAILRRLGRDGRGREGLLRLGPLELDPNGYTVSRSGETLDLTPTEFK
                     ILALLMRRPGQAFTRAQISEAVSTGSHYLASRYIDVHISRLRAKVERDPSSPEIVQTV
                     PSVGYRAARPV"
     gene            15842..17743
                     /gene="dnaK"
                     /locus_tag="VN178_02320"
     CDS             15842..17743
                     /gene="dnaK"
                     /locus_tag="VN178_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWS79832.1"
                     /translation="MAKSIGIDLGTTNSCVAVLEGGDPTVITNSEGERTTPSVVAFDR
                     RSGERLVGQLARRQAVTNPERTVYSIKRFMGRRYSDVKTEAERVGYQVKAAPGGDIRI
                     VVDDKEYSPPEISAMTLQKLKRDAEEYLGEEVTEAVITVPAYFEDAQRQATKDAGRIA
                     GLNVKRIINEPTAASLAYGMDKEEDQTILVFDLGGGTFDVSILELGEGVFEVKATSGN
                     NHLGGDDFDAKVVEWMVSEFKKVEGIDLSKDKMAAQRLVEAAEKAKRELSSTTSTSIN
                     LPFITADQNGPKHMDLTLSRAQFNNLTADLVEATAGPVRQAMDDAGLQPGQVDQVILV
                     GGSTRIPAVQEQVKEITGKEPHKGINPDEVVAVGAAIQAGVLAGEVKDVLLLDVTPLS
                     LGIETKGGVFTKLIERNTTIPTRKSEVFTTADDNQGSVEIKVYQGEREIAAYNKIIGN
                     FQLVGIPPAPRGVPQIEVAFDIDANGIVNVGAKDLGTGKEQRITITASSGLSDAEIQQ
                     MMQDAESHAEEDRQRREEADVRNNADNLVYSIERSLKDVDGKVDPNTKLEIEQAIKET
                     KEALAGGDIEEIKRKQENLMTASHKLSEVLYSQAAQQQEASSAGATDDDLVEDAEVVD
                     EDEDGEERK"
     gene            17815..18450
                     /locus_tag="VN178_02325"
     CDS             17815..18450
                     /locus_tag="VN178_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWS79833.1"
                     /translation="MSNEENKRDPEPEDDTRQEEGPVEEAGGSPVDPASLENGERPGA
                     PEDPEVEPVTGEDPEWDEATILSVELEATRRDRDQYLDALRRLKAEFENSRKRQERER
                     IRVLSMASERLVQELLPVLDNLDRALEAGGDIREGVQATRDQLAEVLGDEGLLPVASD
                     GQAFDPNVHEAVMGQVSEEHEEGTIIQTFQRGYLLNGKPIRTAKVVVAKQA"
     gene            18454..>19213
                     /locus_tag="VN178_02330"
     CDS             18454..>19213
                     /locus_tag="VN178_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWS79834.1"
                     /translation="MEKLDLYKALEVSKEASQEEIRRSYRRLARKYHPDANPGDRQAE
                     ERFKEIQQAYEVLSKPEKRREYDEGPRTFFGSRGAGQPGGAQFEDFSDLLGGFGNLGD
                     IFGRSSGTARTANVKGENITVNIRLKFKDALNGVTTRVGVPVEEACGECRGSGAAPGT
                     APLTCPECGGRGVQSRDQGFFALSTPCSRCGGEGRIIEKPCTVCGGNGRVRKNRQVKV
                     RIPAGAKDGMKVRIPGRGSAGRRGGPAGDLYVVTR"
BASE COUNT         3474 a         5906 c         6288 g         3545 t
ORIGIN      
        1 cggtcaggcg gtagagttcc tcgatgccga ccagggcggc ctcgagcggt tcgagccccc
       61 tagtgtccgc cccaagcgcc tgcgcgatgc gggcgtagcg ctcgggcgcg gctggcgcgt
      121 tgtactcgca gaccggaccg aggacgcggg ccgtcagggc gccgtgggga cagtcgtgga
      181 cgccgcccgc cgactggctc atggcgtggg cggctccggc gctctccgtg ccgtaggcca
      241 tccctccgaa cgtcgccccg tgcgccatga acgtgcgggc ctccatgttc gcgccgtcct
      301 cgcaggcgga ccgcagccac cgcgccacgt actctatggc cgtcagggcg aaagagtcgg
      361 tgaagggctg gtggtaatcg caggtgtagc actcgatccc gtgcgagagc gcgtccatcc
      421 ccgtcgccgc cgttatggca gtcggcagcc cgaccgtcaa ggccgggtct atgagcgcca
      481 cgtgcgcgcc gatcagggcc gtgccgccga cgttgaactt gatcctgcgc ccgtggtcgg
      541 tgatgacggc ccagagcgtg acctcgctgc cggtcccggc cgtcgtgggt accgtgacga
      601 gcggcgggat gcgacgcgtg atcggggtct taccgtattc gtagtcggct atggacccgc
      661 cgtggacgat ctcgacgccc acccccttgg ccgtgtccat cgaactgccg ccgccgaggg
      721 ccaccagccc gtcgcaaccc tcctccctgt agacctcgct cgtccgtccg atgagctcca
      781 cgtcggggtt cggcctgacc ccgtcgaaga cgcaggcgtc cgtaaggcgc tcgagggcct
      841 gctctgcgag gcctgccccg accacgcccc cgtcggttac gagcaacgga cgcctcacgc
      901 ccagctcctc aacgatctcc cccagcctgc cgatggctcc gggcccgtgc accatgcggg
      961 tcgggacctc gacggtgcgc acggtttcga cttcgacgct catacggcct cctcgttcgc
     1021 gttgtgcatc tggttctctt tggtgtccag caatgtcggg gcggaccagt ccggcgcgaa
     1081 cctccgcaga gcgaggcggc gggggtccag gtaaagctcc ggagtaccct cccgctccag
     1141 gaacgagagc gcccgctctc ccagcgccgc tccctcctcg acgtgggact cccacacgtt
     1201 cgattgttcg cccggatccc gcccaagatc gtagagctcg ggcatgtaag cgggtccacc
     1261 gaggatgagg gatcgctccg gcgtggtcac ggtaatcggc atgtagcttg cgatgcgcct
     1321 cggccgggag tcgatggcgg tggtgaactc gcccgccgcg aagtagaggg gccaggagct
     1381 gacgacgaac gggcggtgct cgtccttctc tcccactaga acgtccccga acgactctcc
     1441 ttgcatggac ccgggacgct cgacgccaac gaggtcgagt atggtaggcg cgaggtcggg
     1501 cgcggtcgtc aaggccgggc gacgaccggg ctccagcccg ggtcctcgga tcatcagggg
     1561 cacgttcgtg agctctctgt agaggggtga ccatcccacg gtgagcaacc acgactccgg
     1621 tagccagtca gggacgaccg cgtcctcggt caccgcggcg tcctgatcgt ttatccactc
     1681 ggccttgccg aagtagccgt gctccccgaa gtagaagccg tggtctgaga tgaagaagac
     1741 gagcgtgtcc tcccctagac ccagcacctc cagcttcgcc aggagcctcc cgacccagaa
     1801 atcgaccatc gtgacctcgc cgcagtaggc ggcgtgcgcg agctccacct catcccgggt
     1861 gagtccggcc tcggcccacc tgccgtagct cgggtagatc tggcgtccct cgtaacccgc
     1921 gcggtacagg gaggtgtagt aatcaggcgc gtcccacggc tcgtgcggat cccaggtgtc
     1981 gacgtagaga aagaaccgtt ccttgtagtg tctctccaac cactgctcgg cctccgtgac
     2041 ggtgcgcgac acgagcctgt ccgactccga cctccacgtc tttcggtagt cggagcgctc
     2101 gtgcggcctg gtgtcgtcgc cctggcccct gacccagatg aagtcgtcga agccgcggtc
     2161 ataaccgaac ccgcccctga cgaagaaagg cgtgtccaca acgcccatcg tcaggtaacc
     2221 ggcttcggag agaagcccag gaagcgtggg caggtgatgg ggcaaaggct gccagcccat
     2281 gtgggtgtag gagaaggtgc ccgtcaggat gtccgccctg gccggcatgg tggggaaaga
     2341 ggagatcacg tgccggtcga agacgaccga ggagcgcgcg aactcgtcta ggtgtggcgt
     2401 gtgaatatag gtgtttccga atgcccccag gtggtcccga cggaaggtgt ccgacacgat
     2461 gaccacaatg ttgggtcggg tcgacatatg catacctcct gcataaggta agctcgatgg
     2521 ttaccgatgc aggaggggcg gcgcgcgccg gccggccaga tactggttgc cttccagtaa
     2581 acctttgctg gcaaaagccc cgtaagacca ccagttcaac cgatttcggg actcgaagtc
     2641 gtccgactcg ccgaccagat agggtaggat ccgcacagac ccaggtagcc cgcgaagcgt
     2701 taccttccgg ccgccaaaca tgtagagggg accgcgacgc tcgggtcgaa gcgcgcccga
     2761 aatgtgcgaa gacggtgaga ccccatctcc ggtcgctgtg ttgcgtatag tgcacgcggt
     2821 tgtcatatgg gtaacagata gcactttgtt gctaatatgt caaggggctg gctgagaatc
     2881 ggcgccgcgc gatggagcct ggggctcgag cgcgagaacc gtccagagag atgctgctga
     2941 ggaccgccgt gatcgtaaac cggtagcccg cagtaacgcc cgcactcgtg gaactggcgg
     3001 gaagagatgc gtcctctcgt cagcacagca gcaaactatg ttactattta gataacatgc
     3061 taacctaagc tacctcgtgg acatagagag gatttttgca tgcttcagac ggtcgaccgg
     3121 gcgttacggc ttcttctcct cttcgaggag gttgaccagg agtaccgggt tggcgagatg
     3181 gcggcgatgc tcggggtcga caagagcagc gcgtcgcgcc tcgccgcgac gctggccaac
     3241 cggggtttcc tcgagcgtgc gcccggcagc gaggcgttca ggataggccc ggagctcgag
     3301 cggcttggtg tgctcagcgt gggaggtagc cacaatctca tcgaggtggc aagaagaccg
     3361 atggagaacc tggcggcgga gacggaggag acggtaaatc tcgcccgtct cgaggacggc
     3421 aaggtcgtca acataagcca ggtcgacggt gctcacctgg tcggggttgg cgactggacc
     3481 ggctggaaga cggagcccca cgccaccgcc aacgggaagg tattgttggc gttcgtggag
     3541 aatggggccg agggtttacg ccttcaggca cccctggagg cgtttaccga acagaccatc
     3601 acgaactccg aggatctccg ctctgagcta gatcgcgtca gatccgaggg ttgggcgtcg
     3661 accttaggcg agctcgagga cgggttcaac ggtgtcgctg ttcctgtctc cgacgcgctc
     3721 gggcgctgtc gggcctcgct gagcgtatcg gggccggtat atcggatgcc cccggcgctc
     3781 ttgcccgaga tcgcggtgct ctgcaagaag gctgccgagg agatcagcgc ttctctagga
     3841 aggacgcgta gctcggcctg agacgcgcgt atctttttgc gactggtcgg gccatcaacg
     3901 gtcggattgg ccgggtgatc cggcactgct atgcagcagg tcgccagcag ggtagaatag
     3961 ggcgataggt tgtgcgttcg tggggcagcg aaggctgggg ataggcgtag accgcaacgg
     4021 gagtctgggc aaagaaagcg ccgccggtag cggtgggatc gcgggcggcc tggagcccgc
     4081 attcgagcgg gagtgcgccg gcctcgatcg gatcgcaccg aaggtgcgag tcttcccggg
     4141 tgcagcggtt cttcgagccc ctgcgatacg ttccgcagac gagctccctt gcgaaaaaga
     4201 gccgaagccg ttgggccgga agatgctccc gaagataggt aatcgctaat gctaatacga
     4261 gctgcctgag gaggtggacg gagacccatg ctctctaaac tgttttcacg ggaacccgac
     4321 acgagggtgt tcttcgctac ggacatccac ggttcggaga cctgctggcg caagttcttg
     4381 aactcgggca agcactacga ggcgaacatc atgatcctcg gtggcgatat gacgggcaaa
     4441 gcactcgtcc cgatcgtcga ggaaggaccg ggccgctggc acgcgacgct actcgagaac
     4501 cggagggaat tcgagagcga agacgaggta gaggagtttg aggactcggt caagaggcgc
     4561 ggctactacc ccttccgcac cgacccggac gagatgggcg agctcgagtc ggacgaggag
     4621 atgcgcaatg agctcttcta caaggagatg ctggggacca tagagcgctg gatgcggatg
     4681 gccgacgaga agctcgaagg aaccgacatc gagtgtttcg tgagccccgg caacgacgac
     4741 cagttcgagg tcgatgagat catcgccaac gccaaacgcg tgcggcttgc cgagggtaag
     4801 gtcgtcgagt tcggcgactt ccagatggtc tcgaccgggt ggtcgaaccg cacgccctgg
     4861 gacacctacc gcgaagagga cgaggaggac ctggcagttc gcctgaagaa gatgacggac
     4921 caggtgaccg ccccgccgga gaggaccatc tacaacttcc actgtccgcc ctacggttcg
     4981 ggcctcgacg acgccccgga gttgaccgag gacctgcgcc ccaagcacgg cgggcgttcg
     5041 atcgtgcccg tcggatcgac ggcggtgagg gatgcgataa aggagggaca accggccctg
     5101 gcgttgcacg ggcacatcca cgaggccaag gggaacaccc gcatcggacg cacgctgtgc
     5161 atcaaccccg gcagttccta cgagcagggg cagttgctcg gcgcggtggt gaacctggac
     5221 ggcggcaaga aagtaaagcg cttcgtactg accagcggat gaggaggaga taaatggcca
     5281 gcaacaccac ggcgggttcc ggtgcgggag acctgtttca gaggaaggcg acaggtctcc
     5341 tcagaggatg gtccgtacta gacgggttca tctacgcggc gttcgcaatc aacctgatga
     5401 ctctcggact ctggatcttc tccttcgcgc cgttcatccc tgaagggtcg ctcatctacg
     5461 cgatcatcct ctcgggactc tacctcgttt tccaggccgt aacctacgcg tctctgatcg
     5521 ccgtgatgcc gcgagccggc ggcgactacg tgtggatgag ccgcgtcctc ggcggcggta
     5581 taggcttcgt cctagcggta accggctggt ggttcatcct gtggcactgg gttccgatct
     5641 acgcgaacat cctcaacgtc gaggtcctgg taccgctcgc gacgatcctc aggttcgacg
     5701 gggtcgccaa cttcttcggt gcgcccggtg gtatgttcac cgcctcgatg gcagttgcta
     5761 ttctggcttc gatcctaatc tcgctaggca tgaggaccta cgcccgcatc cagaagttct
     5821 gcttctacgg tggggtcatc gggctggtga tcatgttctc cctcctgctc ttcaactcga
     5881 acgcggactt cgtcggagcg tttaactccc atgcccagga gctctacggc gcggggggca
     5941 acgcttacca gcagaccctc gacgcaggta gcatcgagac gaaccttggg gccctcgcat
     6001 tcggaccgac gctgctcctt atcccgatga tcgtcttctt caacctctgg tccaattggg
     6061 gagccacact ctacggcgag gtcaggggcg cctcggactt ccgcaagaac atctacgcga
     6121 tgggcggcgc ccttatagtt acatcggccg tcgccgtcat catgctgctc ctcttcgcca
     6181 agacgttcgg ctgggacttc tattacgcgg cgagcaacgc ctactggagc ggaaatggcc
     6241 cgatctcggc ctggccttac ccggggctct ttgccgcgtt cctgttcgac agtgccatcc
     6301 tgcagttcat cctgcttgcg ctgcttgcgc tgtggttctt cgggtgggtc gggtcggtgt
     6361 tcctgtcctc cacgcgggtc gtcttcgcca cggccttcga cagggtgttg cccgagtggg
     6421 tgtccaaaac ctcgcgcaac ggcgtcccct actacgcgct ggcgctcatg ctcattccgt
     6481 ccatcccgat cagctacctc tacgccttcg gggagaactt cgcgacctac accctggacg
     6541 cgacactggt catcgcgacg actttcctcg gctcggctat cgcggcggcg gtcctgccgt
     6601 ggcgcaagcc cgagatctac aacgcctccc cgatagcccg ctacaacgtc ctcggcatcc
     6661 cgctggtaac cgtggcggcc tcactgttct cgctgttcct ggtcttctgc ctcttcaagt
     6721 ggctcaccga tgacacctat ggcatcaaca acggccagtc tttgatctac atggcgatcc
     6781 tctacggagt ggcggtggcg gtgtacgtga tcttccgcct gctccggcgc gcccagggga
     6841 tggacctgaa catggtctac gacgagatac cggaggagta gcctttagcg atccgcatag
     6901 gggcatcgcc cctatgcgga tcgcgcattt cagcacttca gcatttcagc tagtgcataa
     6961 agctgaagtg ctgacaagcc tgcgaagcag gcgtgctgag atgctgacta gcttgtgata
     7021 agggccttca gcctgtccct taccgcggcg atgggcacgc gctcctgctc cagggtgtcg
     7081 cggtcgcgga tggtgacctt cttgtcttcg atagtgtcga agtcgacggt tacgcagaag
     7141 ggggtgcctg cctcgtcctg gcgcctgtag cggcgtccga tggagcccga atcgtcgtag
     7201 aagatgcggt agtcaccttt gaggtcgtcg tagagctcgc gggctatggt cgagacgggc
     7261 tcctttttgg agagcgggaa cacggcggcc ttggtcgggg ctatgcgcgg gtgcagcttg
     7321 agtacggtgc gttcctcgcc cctaacctct tcttccgtgt acgcgtcgat gaggaaggcg
     7381 agcatgatcc tgtccgggcc aacagcaggt tctatgacgt aagggacgta gcgctcgccg
     7441 gtagtctggt cgaaatactc caggttctcg ccggagtatg cggcgtgctg cttcaagtcg
     7501 aagtccgtgc ggttcgctat tccttccagc tcggaccacc cgacagaggc gtagttgtac
     7561 tccaggtcca cggtgcgctt ggagtagtgg gagagcttct cctggggatg ttcgtagcgg
     7621 cggaggttct ccactttcag gcccaggttg gtgtaccagt tccagcgttc ttcgatccag
     7681 tactcgtgcc aggcctcgtc cgtgcccggt tcgacgaaga actccatctc catctgctcg
     7741 aactcgcggg tgcgaaagat gaaattgccc ggtgtgatct cgttccggaa tgacttcccg
     7801 atctgggcga tcccgaacgg cacctttacc ctgctggtct ggagcacgtt cttgaagttc
     7861 acgaagatcc cctgcgccgt ctcgggccgc agataggcca gattctcagg ggtcttgacg
     7921 ggccccatgt aggtctcgaa cataaggttg aagggcctta cgggggcgaa gtcccgcccg
     7981 ccgtctacgg ggtcctttac ccggtcgtcc tcctggatga tcttcgttaa ttgctccggg
     8041 ctcaggccct cggcgtctat acctgttgcg tcctcgatga ggtggtcggc gcggtagcgg
     8101 cgtttgctcg tgcggctctc gacgagcatg tcgttgaagg tgagcgtgtg ccccgaggcc
     8161 tcccacaccc tcgggtgcat gattatggcc gagtcgaggc cgaccatgtc ctcgcgcatg
     8221 tggaccatgc ggcgccacca ctcctctttg atgttccgct tcatctcgac gccgagcggc
     8281 ccgtaatcgt atgaggaacg gatgccaccg tagatctccg agctctggta cacgaagccc
     8341 ctacgcttgc agaaggccac tatcttatcc atcgtcacgg tctggggcac tggtcctccg
     8401 aactcttagg gtcaggcaag tatagcccag agagaaaggc ggattgggcc cggcgcaggg
     8461 caggagagaa ccacggtctt ccttcgccga tgacgggcgg agtcaccctg gaccacgcta
     8521 taatgctggc cgtggagagt ctaatcttgg caggtttggg cgttggtctc gcttccgtcg
     8581 cgggggtgag ggccttcgtg ccgctcgcgc tgctatctct gttcgtccgg ctcgggttca
     8641 tcgcggagcc cgacatcttc ggcctgcaga tcggatggac cgtcgtactg gcgctctcgg
     8701 cgctggctgc cgtggagata atgctcgata aggttcgggc cctggacccc gcgttcggct
     8761 tcgtcatgat acccatcagg atggtggcgg gcgccctcct gttcgccgcc gtgtacgagc
     8821 caggagtgct ctttgacgct ggcgcagtac cagggctcgt ggccggtggg gcgatcgccg
     8881 gcgtggtcgc cgggctagag gttatcctgc gcccaccggc gaggagttcg tcggcaggcg
     8941 tctccacggc tttcttgagt gccatggagg acttggtcgc ggttattggc ggagtagtag
     9001 ggttttttct gcccctggtg ccgcttttgt tcgtcgggtt tctgctattc ttcttcaacc
     9061 gcatcaggaa gcggcgtggg aggaaatacg ggggcctccg catccttggc gactagtatt
     9121 cgtcggcgct ctaggagggt gcaatggacg agcgggagat cacgattgtc aaggggggta
     9181 gggagacgcc gttctcccgg gggattctgg cgcagacgct ggcccaggcc ggggcgcgcg
     9241 ccgagctggc gcaccagata gcgagcgagg tccgcgccga gcttttggcc gagcaggtct
     9301 atacggtcga ggaggaagaa gttctcgccc gcgttaggga caagctggtc aagaaggacg
     9361 ccttcgtcgt cggcaggctc gacaagtggc gtgtccttag ggagtcgacc gagccgatag
     9421 tggtcttgat cggcggggcc acgggggtgg ggaccagcac gctcgccgcc gacgtggcca
     9481 ggcgtctgaa catccagagc gtggtcggga ccgattcgat cagggaggtc ttgcggcggg
     9541 ccataagccc cgacctgctc cctttgcttc acaagagttc ctacgagatc aagcgcgagg
     9601 acctgagggt gcccgtggag gaagacgaga tggtcctcta cgggttcagg cagcaggcct
     9661 cccaggtcgc cgtcggagtc gaggccatcg tcgacagggg tttgaaggag gggacgaacc
     9721 tcgttgtcga gggggttcac ctcgtgcccg agatcatcct caaccgctac cgcgaccacc
     9781 cgaacgtgtg cacgctcgtg gtctacctgt cggacgagca cgtgcaccgc tcgcgtttct
     9841 acatcagggc gcttggcacg gcgatgcgcc gtcctgcgga ggagtacatc tcctacttcc
     9901 aggagatccg ccagatccac gactacatcg tcgagagttc cgccagggcc ggtgtgcagg
     9961 ccgtcgagaa cctttccata gagcacacgt cggacgcggc cgtcgagatc gtggccaaca
    10021 gggtctcggg gatcgccgac agggccggcc ggcacgcctc ggcgatgctc ctcgatgggg
    10081 ctacgcccag gcagggtatc cccgtcaccc gcgcgaaaag cggttagaat aaccttcagg
    10141 gcccaggagg agatgagagt gctagacagg cagcgattga gaaccttcgt actcggcggc
    10201 gcggtagggg ccattttggg cgtactcctg gccccccgca gcggtaaaga gctcaggggt
    10261 tcgctcgcca acagggccgg tgaggcccgc gagaggagcc gcgagaccta tttcgacgcg
    10321 caggagcaga tgagggagcg tcttgccgag gtccgcgaag ggtcgcgagg acgccccgag
    10381 gcggtcgacc tcgacgcttc atctcccgcc tccggggaag caggggcgca gaggccctac
    10441 ctcagagacg tctcacgtga tgtcgggtcc gaggctctcg atgaggatgc cgagaggtcc
    10501 gaggagctac gccggaaggt gagggagacc agggaacggc tgcggggacc gaaggagggc
    10561 ggtccggagc gatgaaaccc acgtctttcg tgccgatggt cggtgagatc tcggccgagg
    10621 atgccggcga ggtgcccggt cttggggccg tgatctcgta ctggaccggc gataggtact
    10681 ccgccgaagg gtggttccgc gagctgaatg acgtctgggg aacggcgggt ttctccgtgc
    10741 ggcgcggtgg ggaggtccag ggattcgtgg tctacgggcc gcccgaacgt ctggcgcgcg
    10801 cggcgcgttt tcccctgggg ccgatcgatg acgaggccgt gctgctggcc tacgtcgggg
    10861 gagacgctcg tacgcggcgc cggctgttgg taaggatgct cagggatctc aggcagcggg
    10921 gcgtcgggag ggtcgaggcg atagccagcg accgtggggt gtccaaccac gtgccgactt
    10981 ctttccttct ggagagcggc tggcagcccg tgcgacgcgc cgcttaccgg atgagctact
    11041 acacgctcgc gcacatcgac ctgaagagcg ccgtcgaggt gggggagctg gcacgcgcgc
    11101 tggtggggcg ggtgaaactg cccgggctca agtcgcccgt gcccggtgcg ctggcccgca
    11161 ctacgggcga gcaaagaagc cggtgaggcg agtccgcggg gaccacggtc ccgcggactc
    11221 gcgcacttca gcacgcttcg ggcctttcag gccctcgctt gtcagcattt cagcaagaac
    11281 gaaggcctga caagcgaagc gtactgacag cgtcgggcgt gagcctcgtg cacgatgggt
    11341 tatcggcttg tcaacagtcg tcacgtactt cagcaaacgc aacctgaagt gctgaaatgc
    11401 tgacttgctt tcaggggggc aggatcaccg tcctgaccgt cgagtcaccc tctggcgtgg
    11461 ttgccctaat ctccaggatc tcacgcgatg gttcggggtt ctccgttggt ggcttcgcca
    11521 caggatcttc gcgggccgga aggtcgaact cgaagcgcac actggctgtc tcgccggccg
    11581 gtatcttctc gaccgtctgc ctgtagtagc gacggtccga gagtgccgtg ttctcggagg
    11641 cgacgctcag ggtcatgttc acatccgaga gctgctcggt cccctcgttc tctacttcga
    11701 gctccaggct ctgatccggg aggggttcga gagatgcgag tcgttctctg tccagtatca
    11761 ggtccacggc ttgtcctgac tctccccttg cctggtccgg attcagacgc ttctcggaag
    11821 gggcggtagc gaggttcgcc aggatcagga atgctcccgc cgtgagcagg aaggccaggg
    11881 tcgcgccgga gagccagaat actatgtccc tcatcgcggg gcatcctcgg ggccgccgac
    11941 cggtatctcg tagagattat agaaatcttc gaggagcctg acagcggggg ccgcatgcac
    12001 ctcgggctcc gtagatggtg tgccccgagc gactacgact acgctctggc cgccgggcga
    12061 ccacgcgagg tcgtcaggcc cggtctttat ctccatgtcc tcgttcgtcg ttacggccgt
    12121 caggtcctcc gctagggggt caagcacgta caggttggtc ggggcctgcg ggctgaggcg
    12181 tccgatgatg gccaatcgtt cgccgtccgg agagacgcgg atgctcgttg ctacgaagtc
    12241 ctctcccaca ccgggcgcgg gttccggcgc cccgccctcc tcgggcacgc gaaagatctc
    12301 gtagagtggc tctgagccat ccctatccag gggcgtattt ttcacccctg ccacaaagta
    12361 gaggtactgc cccgtccact ggggcgtgcc gagtatctcc cggttgccgt ccagccgcgt
    12421 tatctctcgt actcgcccct tccgaagatc gaacatgtgc agcctcgaag tctggaggtc
    12481 tccgggagga cgcacggtca ggacggcctc gtcaccgtca ggtgagacgg agagggatgc
    12541 cacgcggccc ttgatggact cggagtagag cctgcggacg tccccattac ctcgcgtcag
    12601 cgcgagtctg ctctcggaag ccccgcgacc gcgggccagg atcaaccccc ccctgccggt
    12661 gccgatcgcc gaaacttcct tctcgatcct ctctagctcc agcgaatctc cctctgctcg
    12721 cgtcctgtag agggagctag ggctctcctc tgtccccgtg ggcgacgccg cgccgagtat
    12781 catcagggta tcttccgaga tccactccgt atcttctgcg gtgaaatccc tggtcgtcca
    12841 gcgtctcggc tcttctgagt ccgtcggcct atctaccacg tagccgtcta ccgtaaatgc
    12901 gatcctggcg cccccaggac tccagctcgg cgagcctttg tagcctggcc ccgaactgag
    12961 ggcccgagtc tccgggccga gtccggggtc cagcctgccc gctgccggga caaggggcgc
    13021 gatggaaact tccttctcgg cgtgggtgca ccccgccata aagacggccg cgaccgagag
    13081 gactgcgacc cgttttgccg gccctattct agagagcatg acttacggag cgggagagga
    13141 atgccttggg gagtctgacc tcgaagacgg agcccacgtc gacctcgctc tcgacggaga
    13201 tgctgccgcc gtgcatctcc actatctgcc tgcttatggc gagcccgagc ccgcttcccc
    13261 ctgtcgcgcg ggaacggtcc ttgtccaccc tgtagaaacg ttcgaatagc tggttcagat
    13321 cctcttcggg gatgccaacg ccagtatcac ggatgcgcac ggtcagagtc gagtcgtctt
    13381 cctctatctc tacatccacc ctggcgcccg agtcggagta ctttatcgcg ttgtccagca
    13441 ggttgaggaa gacctggtag agcttctgga cgtcgcagat gcacctgtgc tcgtccgcgt
    13501 tatcgaagta tatcttgatg ccgaacctct gggccttggc ctctacggac tctattgccg
    13561 tctggacgat cgtactcatg tttgccggcc ctacctcgag ctggtacttc caggagtcca
    13621 tctgggcgag ggtcatcagc tcctcgacca tcccccttat gcgtttcaat tcctgatcgg
    13681 ccagctcgag cgcccggtgc tccagcgggt cgagacggtc acgtgcccgc tccagcaggc
    13741 cgaccgcgct ctggatggtg gtcagtgggg tcctgagctc gtgggaaaga tccgagacga
    13801 aggcggtctt ggccttctct agcgagcggt gctcggttat gtcccttacg gccaggatga
    13861 tgccggcgtc gccgttgggc gcgttggtgg gctgccccct gtccatctgg gcggcgtaag
    13921 cccagaaagc cttctcccca agctcgacct cccttaccgc aaccccgttg gcggccgctt
    13981 cgcgcaggca cttgaggact gcgcttggga ccccggtctc tcccagccta cgccccctgt
    14041 ccgaggccct gatgcccagc atcctggccg ctgccgcgtt gaccatcgta acccgctcgc
    14101 cggagtccgt ggccacgacg gcctcgggag aggcctccag gacggcctcc agacggctct
    14161 tttgctcctg gatctccccg acgtagtgct caatctcctc ggccatgtag ttgaaagatc
    14221 ttgccacctc gccgagctcg tccctgctct tgacgggcac ggtcgccgcg taatcccctg
    14281 aggcgactct tatggccgcg tccctggtct cggtgagggg acgcgtgacc cgacgcgtga
    14341 gaagcaagct cgccgccccg gcaaggagga tgctcgtcag tatggccccc aggatgccgt
    14401 agcgcaggta ggccagcgta tctccgagct cgtcgcgggg aacgtggtag atcaggactc
    14461 ccttctcggt accgtcgtag gccgtgagcg gccacaccgc tacgtacctc gaacggccct
    14521 cgctccacac gagcctgccc tctcctcctg gcgatgaggc gatctcctcg agctcggctt
    14581 tgtcgagtcc cccgaatgct tccctggggt cgaggggatc cccgaggccg tcgcgggcgg
    14641 ccagaggttc catgtccggt cctaggtaga gcacttcgag gcccgtcgga tcggcgaccg
    14701 cctggacgag ctcatcggcg tagtcgggcg ggggcgggag agtcttatct tcagggtcgc
    14761 gcagaccttc caggaagtcc tgcgcgccgg agttcgcggt ctgggagcgt tgcctgagca
    14821 gcgtgttctc cgcgtgtacc agcctgtcct gggcgaagta gtacaggacc agtccgaata
    14881 taaggctggt aacggatgag atcccgacga gggccacgaa gatcctggcc ttgaagttca
    14941 acgccggcgg gagtccccta tcttccctct tttctatgcc catcctccgt ctcttagacg
    15001 ggcctcgccg ccctgtagcc gacgctgggg acggtctgca cgatctccgg actcgaaggg
    15061 tcccgctcga ccttggccct tagcctgcta atgtggacgt ctatgtagcg gctcgccagg
    15121 taatgcgacc ccgtggagac ggcctctgag atctgggccc gcgtaaaggc ctgcccggga
    15181 cggcgcataa ggagcgcgag gatcttgaac tcggtgggcg tgagatccaa cgtttccccc
    15241 gaacggctga cggtgtagcc gttgggatcc aactccagag gaccgagcct caataacccc
    15301 tccctgcccc tgccatcccg gcccaaacgc ctgaggatcg ccttgatcct ggccagcaac
    15361 tctttggtgt cgaagggctt ggtgatgtaa tcgtcggccc cgagctccag cccgaccact
    15421 ttgtccacgg actggctctt cgccgtgagt aagactacag ggacgtcgct gtgctcgcgc
    15481 atcttcttca agacctcgaa tccgctcatc ctcggcaaca tgatgtcgag caccacgaga
    15541 tccaaaccct cccgcccgag gatccggagc gcctcagccc cgtcctcagc cgtcactacc
    15601 cggtaaccct cagatgccag tacgatggac gtgacctcca aaagcgccgc gtcatcatcg
    15661 accaacaaga tcgtctcgtc caatctgact ccccgcaaac ttaccgccag cgtccaccgt
    15721 atcctacctt atacccgccc tcctccgtaa caccaaagtg cggtgagaaa cttgcaagcg
    15781 taacactcag attttatact gatgacactt tagataacgt tatctcgtgg gaggtatgcg
    15841 catggccaag agcattggca tagatttggg gacgaccaac agttgtgttg cggtcctcga
    15901 gggtggggat ccgacggtga tcacgaactc cgagggggag cggacgacgc cttcggtcgt
    15961 ggccttcgac aggaggagtg gcgagcggct ggtggggcag ttggcccgga ggcaggcggt
    16021 cacgaacccg gagcgcacgg tctattcgat caagcgcttc atgggacggc gctacagcga
    16081 cgtgaagacc gaggccgagc gcgtgggcta ccaggtcaag gcggcccctg gcggggacat
    16141 tcggatagtg gtcgacgaca aggagtactc tccgcccgag atctccgcga tgacccttca
    16201 gaagctcaag agggacgccg aggagtatct gggcgaggag gttaccgagg ccgttatcac
    16261 ggtgcccgcg tacttcgagg acgcccagcg ccaggcgacc aaggatgccg gaaggatcgc
    16321 cgggttgaac gtcaagcgga ttatcaatga gccgacggcg gcgtcgctgg cctatggtat
    16381 ggacaaggag gaggaccaga ccatcctcgt cttcgacctt ggtggcggta cgttcgacgt
    16441 ctccatcctg gagctcggcg agggtgtctt cgaggtcaag gctacgagtg gcaacaacca
    16501 cctcggcggc gacgacttcg acgccaaggt cgtcgagtgg atggtctcgg agttcaagaa
    16561 ggtagagggc atagacctct ccaaggacaa gatggccgcc cagcgcctgg tcgaagccgc
    16621 ggagaaggcc aagagggagc tctcttcgac caccagcacc tcgataaacc tgcctttcat
    16681 cacggcggat cagaacggcc cgaagcacat ggatttgacg ctcagcaggg cgcagttcaa
    16741 caacctgacc gccgacctcg tcgaggccac ggccggtccc gttcgccagg cgatggatga
    16801 cgccggtctg caaccaggcc aggtcgacca ggtgatactc gtcggcggct cgacccgcat
    16861 ccccgcggtc caggagcagg taaaggagat cacgggcaaa gagccccaca aggggatcaa
    16921 ccccgatgag gtcgtcgccg tgggggcggc catccaggcc ggcgtgctcg cgggagaggt
    16981 caaggacgtg ctgctcctgg acgttacgcc gctctccctc ggtatcgaga ccaagggcgg
    17041 ggtgttcacc aagctcatcg aaaggaacac cacgataccc acacgtaaga gtgaggtatt
    17101 caccaccgcg gacgacaatc aaggatcggt ggagatcaag gtctaccagg gcgagcgcga
    17161 gatcgcggcg tacaacaaga tcatcggcaa cttccagctc gtcggcatcc cgcctgcgcc
    17221 caggggtgta ccccagatcg aggtcgcttt cgacatcgac gcgaacggca tagtcaacgt
    17281 cggggccaag gacctgggga cgggcaagga gcagaggatc acgatcacgg cctccagcgg
    17341 cctctcggac gcggagatcc agcagatgat gcaggacgcg gaatcgcacg ccgaagagga
    17401 caggcagcgc cgcgaagagg ctgacgtccg caacaacgcc gacaacctgg tgtactccat
    17461 cgagcgttcg ctaaaggacg tggacggcaa ggtcgacccg aacaccaagc tggagataga
    17521 gcaggccata aaggagacca aggaggcgct cgccggcggc gacatcgagg agatcaagcg
    17581 caagcaagag aacctgatga ccgcctccca caagctctcg gaggtcctct acagccaggc
    17641 ggcccagcag caagaggcca gctccgccgg ggcaacggac gatgacctgg tggaggacgc
    17701 cgaggtcgtc gacgaggacg aggacggcga ggagcgcaag taaagaggac cgggcggccg
    17761 gggtgcagac attccggccg cccgcgcggc atgctacagg aggactaggc agctttgagc
    17821 aacgaggaga acaagcgaga cccggaaccc gaggatgata ctcggcagga agaagggcct
    17881 gtagaggagg ccggaggctc ccccgtcgac cccgcctccc tcgaaaacgg ggaacgcccc
    17941 ggcgcgccgg aagaccccga agtcgaaccg gtcaccggcg aggacccgga atgggacgag
    18001 gccacgatac tctcggtaga gctggaggcc acgcgcaggg accgggacca gtacctcgac
    18061 gcgctgcgtc gcctgaaggc cgagttcgag aactcgcgca agcgccagga gcgggaacgt
    18121 atccgcgtgc tatccatggc atcggagagg cttgtacagg agcttctccc tgtcctcgac
    18181 aacctggacc gtgcgctcga ggccggggga gatatccggg agggcgtgca ggccaccagg
    18241 gaccaactcg ccgaagtact cggcgacgag ggccttttgc ccgtcgcctc tgacggccag
    18301 gcgttcgacc cgaacgtcca cgaagccgtc atgggccagg tctcagagga gcatgaggaa
    18361 ggcacgatca tccagacctt ccagagaggc tatctactca acggtaagcc gattaggacg
    18421 gcgaaagttg tagttgccaa gcaggcttag ggtatggaga agttggatct ctacaaggct
    18481 ctcgaggtat ccaaggaggc ttcgcaggag gagatccgcc gctcctaccg taggcttgct
    18541 cgcaaatacc atcccgacgc caacccgggc gacaggcagg cggaagagcg cttcaaagag
    18601 atacagcaag cctacgaagt cctctcgaaa cccgagaaaa gacgggaata cgacgagggt
    18661 cctcgtacct ttttcggctc ccgaggcgcc ggtcagccgg gcggcgcgca gttcgaagac
    18721 ttctcggatc tgctcggcgg tttcgggaac ctcggggata tcttcggacg ttcgagcggg
    18781 accgccagaa ctgccaacgt aaagggcgag aacataaccg taaacatcag gttgaaattc
    18841 aaagatgctt tgaatggtgt aacgacgcgg gtcggggtac cggtcgagga ggcatgtggc
    18901 gagtgccggg gctcgggggc ggcacccggg acggcgccgc tgacgtgccc cgagtgtggg
    18961 ggcaggggtg tacagagccg agaccagggt tttttcgcgc tctcgacgcc gtgctcgagg
    19021 tgtggggggg agggtaggat catcgagaag ccgtgcacgg tctgcggcgg caacggcagg
    19081 gtgcgtaaga acaggcaggt caaggtcagg attccggccg gggcgaagga tggcatgaag
    19141 gtcaggatcc ctggcagggg aagcgctggc aggagaggag ggcctgcggg agacctctat
    19201 gtcgtgacgc gcg
//
LOCUS       DATMVF010000051         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_557216, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000051 DATMVF010000000
VERSION     DATMVF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557216"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="VN178_02335"
     CDS             <1..1716
                     /locus_tag="VN178_02335"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HWS79835.1"
                     /translation="ELNYAENIFRHAPYRQDEPAILHQSEVRPLGEVSWAELQEKTAA
                     LAAGLREMGVGRGDRVAAYLPNIPEAVIALLACASIGAVWSSCSPDFGAGSVVDRFEQ
                     IEPEVLLAVDGYRYGGKDYDRLDVVAKLQDEIPTLQKTVVLPYLSGDPDTSSLQNVVM
                     WDDLLAGHQGAELAFEQVPFDHPLWVLYSSGTTGLPKAIVHSQGGILIEHLKKVVLHI
                     DLGPDDRFFWFTTTGWMMWNLVVAGLLSGSTILLYDGNPGYPDMNVLWEFAEKTGMTC
                     FGTSASYITACMKADIEPGRDFDLTNLKSIGSTGSPLPPEGFEWAYEHVKEDLWLFST
                     SGGTDLCTAFVGGVPLLPVRAGELQARSLGAKVEAFDEEGNAQVEDVGELVITEAMPS
                     MPIYFWNDPEGERYSESYFDVYPGVWRHGDWIKVKDNGACVIYGRSDSTINRGGIRMG
                     TSEIYSAVDKVEEVSDSLVVDVPRDGGSSFMPLFVVLHEGVELDEDLKNTIKESIKEN
                     TSPRHVPNEIFAVPEIPTTLNGKKLEVPVKKILSGTPPEKAASKESMSNPESLDRFVE
                     LAGNM"
     gene            1924..3285
                     /locus_tag="VN178_02340"
     CDS             1924..3285
                     /locus_tag="VN178_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79836.1"
                     /translation="MTDSAGQVAAPSPSTVRKVALAAMAGSAIEWYDFFIYLTAAALV
                     FGPLYFPGGSEVAGVLASFSTAAVGFVARPIGGILFGHFGDSLGRKPTLVIALLLMGT
                     ATTLVGVLPTFATIGVAAPILLFVLRFMQGLAVGGQWGGAVLLATEYAPEGKRGFYGS
                     FAQAGVPVGLVIGNTVFLALSALFGEAAFAAWAWRIPFLLSVILIAVALYIQLRLEDT
                     PVFRQLEERQHEGEETESRSPVLEVITQHPKQILLAAGAFFVVNGGFYILITGMLDYG
                     TRVLGISQNVMLAAVLISAVVEGIAIVGMSALSDRVGRQTVFMAGAVLLAIWSFPLFW
                     LVNTGSFILIVIALCVAQFFQGMMYGPQAALFAEMFSARVRYSGASIGYQGASVFAGG
                     LAPIIMVWLLDTTGTSLSVSFYLLAMCAITFFSVYLITETYEDEMTRDQSHEEAEEEA
                     STT"
BASE COUNT          626 a         1063 c         1105 g          666 t
ORIGIN      
        1 gagctgaatt acgccgagaa catcttcaga cacgccccat accgccaaga tgagcccgcg
       61 atcctgcacc agtccgaggt gaggcccctg ggtgaagtca gctgggccga gttgcaggag
      121 aagacggccg cgctcgccgc cggcctgagg gagatgggcg tcgggcgggg tgacagggtc
      181 gcggcttacc tgccgaacat ccccgaggcg gtaatagccc tgctggcttg cgcttccata
      241 ggtgctgtct ggtcgagctg ctcccccgac ttcggtgccg gcagcgtcgt cgaccgcttc
      301 gagcagatag agcccgaggt ccttctcgcg gtcgacggct accgctacgg tggtaaggat
      361 tacgacaggt tagatgtcgt cgcgaaactg caggatgaga tcccgacgct acagaagacc
      421 gtcgtactcc catacctttc aggagatccg gacacgagct ctctgcagaa cgtcgttatg
      481 tgggacgacc tccttgccgg gcaccagggg gcggagctcg ccttcgagca ggtgcctttc
      541 gatcatccgc tgtgggtact ctactcctct ggaacgacgg gtcttccgaa ggcaatcgtg
      601 catagccagg gcggcatcct catcgagcac ctcaagaagg tagtcttgca catagacctc
      661 gggccggatg accgcttttt ctggttcacc actacgggct ggatgatgtg gaacctggtg
      721 gtagcgggcc tgctcagcgg ctctaccatc ctgctctacg acggcaaccc aggctacccc
      781 gacatgaacg tcctctggga gttcgccgag aagaccggca tgacctgttt cgggaccagc
      841 gcgagttaca tcacagcctg catgaaggcg gatattgagc cgggacgaga cttcgacctg
      901 acgaacctga agtctatagg ttcgacgggc tcgccgctgc cgccagaggg tttcgagtgg
      961 gcctacgagc atgtcaagga ggacctctgg ctcttctcca ccagcggcgg caccgacctg
     1021 tgcaccgcgt tcgtcggcgg ggtaccgctc ctgcccgtcc gtgcggggga gctccaggcc
     1081 cgttccctgg gggcgaaggt cgaggccttc gacgaggagg ggaacgcgca ggtagaggac
     1141 gtcggggagc tagttatcac ggaagccatg ccttccatgc cgatctactt ctggaacgat
     1201 ccggaagggg agcgttacag tgagagctac ttcgatgtct atcccggcgt gtggcgtcac
     1261 ggggattgga tcaaggtgaa ggataacggg gcctgcgtga tctatggtcg ctccgactcg
     1321 accataaacc gtggcggcat aaggatgggg acgagcgaga tctactccgc cgtcgacaag
     1381 gtcgaggagg tgtcagatag cctcgtcgtc gacgtcccaa gagatggagg aagctcgttc
     1441 atgccgctct tcgtggtctt gcatgaaggc gtggagctcg acgaagactt gaagaacacg
     1501 ataaaggaga gcatcaaaga gaacacctca cccaggcacg tgcccaatga gatcttcgcc
     1561 gtccccgaga tacccacgac cctcaacggc aagaagcttg aggtgccggt caagaagatc
     1621 ctctcgggca ccccaccgga gaaggccgcg agcaaagaat ccatgagcaa ccccgagtcc
     1681 ctggaccgct tcgtggagct ggccgggaac atgtgatccc accgtgcccg gcgcgttcga
     1741 gcgcgccggg cacattttcg cgtctcccag gcaacgattg actctacccc gcaattcttc
     1801 taatctgccg caagggaagc atcgaaaaga ggggggaaag cgcatggccc ggtactggag
     1861 agagtcaccc tcgagtcctg ctcgtgggat cgtccgtaac acggggttcg aggggaggct
     1921 tgtatgacag attccgctgg gcaggtggcg gcgccgagtc cgtccacggt gcggaaggta
     1981 gccctggctg cgatggccgg gtcggcgatc gaatggtacg acttctttat ctaccttacg
     2041 gcggccgcgc tggtcttcgg acccctgtac tttcctggtg gtagcgaggt agcgggcgta
     2101 ctcgcctcgt tcagcaccgc ggcggtcggg ttcgtcgcgc gtcccatagg gggcatcctc
     2161 ttcggccact ttggggacag cctcggacgc aaaccgaccc tcgttatcgc gctgctgctg
     2221 atgggcacgg caacgaccct ggtcggcgtc ctgcccacct tcgccaccat aggcgtcgca
     2281 gcccccatac tgctcttcgt gcttcgcttc atgcagggcc tcgccgtcgg cggccagtgg
     2341 ggcggcgcgg tcttgctcgc cacggagtac gcccccgaag gcaaaagggg tttctacggc
     2401 agctttgccc aggctggcgt cccggtcggg ctcgtgatcg gcaacaccgt cttcctcgcg
     2461 ctctccgcgc tcttcgggga ggcggcgttc gccgcgtggg cctggcgcat acctttcctg
     2521 ctcagcgtta tactgatcgc cgtggcgctc tacatacaac tgcgcctgga ggacacgccc
     2581 gtgttcaggc agctggagga gaggcagcac gagggtgagg agaccgagtc ccgctcgccc
     2641 gttctcgagg ttatcaccca acatcccaag cagattctgc tcgcggcggg cgcgttcttc
     2701 gtcgtaaacg gtggtttcta catcctgatc accggcatgc tcgattacgg taccagggtc
     2761 ctcggcatct cccagaacgt gatgctggcc gcggtcttga tctccgccgt ggtcgagggc
     2821 atcgcgatcg tgggcatgtc ggcgctctcg gaccgcgtgg gccgtcagac ggtattcatg
     2881 gccggggccg tcctgctggc gatctggtcc ttccccttgt tctggctcgt caacacgggg
     2941 tcgttcatcc ttatcgtcat cgctctgtgc gtcgcccagt tcttccaggg catgatgtac
     3001 gggccgcagg cggcgctctt cgccgagatg ttcagcgcgc gggtgcgcta ctcgggggcc
     3061 tccatcggct accagggagc ctcggtcttt gcgggagggc tcgcgcccat catcatggtc
     3121 tggctgctgg atacgacggg tacctccctt tccgtctctt tctacctgct cgccatgtgt
     3181 gccatcacct tcttctcggt ctacctgatc accgagacct acgaggacga gatgacgagg
     3241 gaccagtcac atgaggaggc cgaggaggag gcctcgacaa cgtagcagcg ccccaagaag
     3301 gggagcaatg ccgctctagg aggcaagttg tacgagaccg tgctgtacga gaaagccgcc
     3361 gcggtggcca acatcgccct gaaccggccg aagaagctga actccttcga ctccacgatg
     3421 cacgaggaac tgtacgacgc actgggtgag gccgccggcg
//
LOCUS       DATMVF010000052         4153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_283346, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000052 DATMVF010000000
VERSION     DATMVF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283346"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VN178_02345"
     CDS             complement(<1..1186)
                     /locus_tag="VN178_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWS79837.1"
                     /translation="MAERLAPQKGEWIDRTRELHFSFEGKKHTGFAGDTISSALWASG
                     RRLLGRSFKYHRQRGIFSFADHDANVMMQDRSLPNIRGDVTPLEEGQKLRAVNTVGGL
                     KMDLGQINNRLARFLPVGFYYKSFHKPARLFPHYERLIRKAAGLGYVTPGEERRHWGK
                     GYGFADVLVVGAGPSGLAAAAAAGEAGAKVILVDENARPGGSLTYAKGYDPAADGRLA
                     ELLRRVEKYPNIEYRADTVASAHFEEHWIPLVHRQHGITKMRAGSVVVAGGAMEQPAV
                     FRNNDLPGVMLGSAAQRLIHRYAVKPFERSVVLAANRDAYGVVLDLLGAGVEVAAVID
                     LRPGGEESELGEAVRSAGVAVYAGHCVYEAVSAKKGVGVGRAVIAPLDVQNNPVPSRA
                     FEV"
     gene            complement(1179..1445)
                     /locus_tag="VN178_02350"
     CDS             complement(1179..1445)
                     /locus_tag="VN178_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HWS79838.1"
                     /translation="MKILTCPINGPRPIQEFLFGGEFREMPDPDAATDARWTDYVYNR
                     DGAPGPRREWWYHLASGTWFIAERDTGTNDVKGTYLYTDEVEHG"
     gene            complement(1452..2693)
                     /locus_tag="VN178_02355"
     CDS             complement(1452..2693)
                     /locus_tag="VN178_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS79839.1"
                     /translation="MPYRLLKYGLSSKHPDERMFTTPSELKKSYDAVIIGAGGHGLAA
                     AYYLARDFGITDVAVLEKGYIGAGGTGRNTAIIRSNYLTPEGVKFYDESIRLFKDLSG
                     EFDLNLFYSERGHFTLAHSDSAIRTMRWRAEVNKHLGVDSEYVEPEDVKVACPPIDLT
                     CGGDAPIMGALYHPPGAIARHDAVAWGYGAGADERGVEIHQGTEVTGIDVKKGRVVGV
                     RTSGGYVRTPKVLSAVAGWTPSIADMVGLRTPLTVRPLQACVTEPLKPFLDKIMVSGS
                     LHVYVSQSSRGELVMGGSTDPYELNSMRSTLEFAEGLSGHMLDLFPSLSNVKILRVWA
                     GLCDMTPDFAPIMGRTPVGGFYIDAGWGTWGFKATPVSGKTMAQTLATDREPELIHAF
                     RLSRFEELELTGEKGAASVGH"
     gene            complement(2861..>4153)
                     /locus_tag="VN178_02360"
     CDS             complement(2861..>4153)
                     /locus_tag="VN178_02360"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWS79840.1"
                     /translation="GDFNAIALANNLLAALVDNHVHQGNDLGLDLRRIVWKRVMDMND
                     RALRDIVVGLGGPTNGFPREDAFDIVVASEVMAILCLATSLEDLKERLGNIVVGYTRD
                     ERPVVARDLDAHGAMAVLLKDAIKPNLVQTLEGNPAFVHGGPFANIAHGCNSVMATKT
                     ALKLADYVLTEAGFGADLGAEKFVDIKCRMSGLRPSMVVLVATMRALKFHGGAEPGEL
                     EKEDLGALEGGLANLERHINNVRNHFGLPCVVAINRFPSDTEAEISLLKDGVAHQGAR
                     VVLTNHWAEGGVGAEALAHAVVETVQSVPARFRFVYDEEDSLWDKISAVATKIYGASE
                     VVADTKVRRQIRKFQEDGYGHYPVCIAKTQYSFSTDPSLRGAPSGHVLSIREVRLSAG
                     AEFVVAVCGDIMTMPGLPKSPASERIDLGEKGEVIGLF"
BASE COUNT          666 a         1436 c         1283 g          768 t
ORIGIN      
        1 cgacctcgaa ggccctgctc gggacggggt tgttctgcac gtcgagaggc gcgatcacgg
       61 cccgcccgac cccgacgccc ttcttggctg agacggcctc gtagacgcag tggcccgcgt
      121 acaccgcgac gcctgcgctc cgtacggcct cgccgagctc cgactcttcg cccccggggc
      181 gcaggtcgat cacggcagcg acctcgacgc cggccccgag aagatctagc accacgccgt
      241 aggcgtcgcg gttggcggcg aggacgacgc ttctctcgaa cggcttgaca gcgtagcggt
      301 ggatcaagcg ctgcgccgcc gacccgagca tgacgccggg caggtcgttg ttcctgaaga
      361 cggcgggctg ctccatcgcc ccgccggcga cgacgacgga cccggcccgc atcttggtga
      421 tcccgtgttg cctgtggacg agcggtatcc agtgctcctc gaagtgcgcg ctcgccaccg
      481 tatccgcccg gtattcgatg ttggggtact tctctaccct tcggagcagt tcggcgagcc
      541 tcccatccgc cgcagggtcg tagcccttgg cgtaggtgag ggacccgccg ggcctggcgt
      601 tctcgtcgac gaggatgacc ttagccccag cctcgccggc cgcggccgcc gccgcgagcc
      661 cggaaggacc ggcgccgacc acgagtacgt cggcgaaccc gtagcccttg ccccagtggc
      721 gccgctcctc gccgggcgtg acgtacccca gcccggcggc cttgcgtatg agcctctcgt
      781 agtgcgggaa gagcctggcc ggtttgtgaa acgacttgta gtagaagccg acgggcagga
      841 atcgcgcgag gcggttgttg atctgaccga gatccatctt gagcccgccg acggtgttga
      901 ccgccctgag cttctgcccc tcctcgagcg gcgtcacgtc gccgcgtatg ttcggaaggg
      961 agcggtcctg catcatgacg ttggcgtcgt ggtcggcgaa gctgaagatc ccgcgctggc
     1021 ggtggtactt gaagctgcgc cccagcaggc gcctccccga agcccagagg gcactgctta
     1081 tggtgtcccc ggcgaagccg gtgtgcttct ttccttcgaa agagaagtgg agctccctgg
     1141 tgcggtcgat ccactcgccc ttctgcgggg ccaggcgttc agccatgctc cacctcatcc
     1201 gtgtacaggt aggtcccttt cacgtcgttg gtgccggtgt cgcgctcggc gataaaccag
     1261 gtgccgctcg cgaggtgata ccaccactcc cgccttgggc ccggcgcccc gtcccggttg
     1321 tacacgtaat cggtccaccg ggcgtccgta gcggcgtcgg ggtcaggcat ctcccgaaac
     1381 tccccgccga agaggaattc ctgtatggga cgcggaccgt ttatggggca ggtcagtatc
     1441 ttcatctctt cctagtgccc cactgaggcg gcgcccttct cgccggtcag ttccaactcc
     1501 tcgaacctgg agagcctgaa ggcgtggatg agctccggct cccggtcggt ggcgagggtc
     1561 tgggccatcg tcttgccgct cacgggggtg gccttgaaac cccaggtccc ccacccagcg
     1621 tcgatgtaga atccgcccac cggggtcctg cccatgatcg gtgcgaagtc cggcgtcatg
     1681 tcgcacagac ccgcccacac gcgtaggatc ttcacgttcg acagggacgg gaagaggtcg
     1741 agcatgtgcc ccgagagccc ttcggcgaac tccagcgtgg agcgcattga gttcagctcg
     1801 taagggtccg tcgacccgcc catcacgagc tccccgcgcg aggactggct cacgtacacg
     1861 tgcagactgc cggagaccat gatcttgtcg agaaagggct tcaagggctc cgtcacgcac
     1921 gcttgcaacg gcctcaccgt gagcggggtg cgcagtccga ccatgtccgc gatcgagggg
     1981 gtccatccgg ccaccgccga gagaaccttc ggcgtcctca cgtacccccc gctggtgcgc
     2041 accccgacga cccggccttt cttcacgtct atcccggtaa cctcggtgcc ctggtggatc
     2101 tccacgcccc gctcgtcggc gccggccccg tagccccacg ccaccgcatc gtgcctggcg
     2161 atggcaccag gcgggtggta aagggcgccc attatcggtg cgtccccccc gcacgtcagg
     2221 tctatcgggg ggcaggcgac cttcacgtcc tcgggctcga cgtactcgga atctaccccc
     2281 aggtgcttgt tgacctcggc ccgccagcgc atggtccgta tcgcggagtc gctgtgcgcc
     2341 agcgtgaagt gcccgcgctc ggagtagaac aggttgaggt cgaactctcc cgaaaggtcc
     2401 ttgaacagcc ggatgctctc gtcgtagaac ttcaccccct ccggcgtgag gtagttcgag
     2461 cgaataatcg ccgtgttccg accggtcccc cccgccccga tgtacccctt ctccaacacc
     2521 gcaacgtccg ttatcccgaa gtcgcgagcg aggtagtaag ccgccgcgag tccatgcccc
     2581 ccggcgccga tgatcaccgc atcgtaactc ttcttgagct ccgagggcgt cgtgaacatc
     2641 cgctcgtccg gatgcttgct gctcaacccg tactttagga gccggtaagg catgcactct
     2701 tcctcaatag ctttctccct gcggcctaca agccatcata gcccacccgt tcatgggttt
     2761 ctatttcctg tagatgggcc gagagaacct gtgcaacccg gacaggaacc ggcgacagag
     2821 agtggcctac ctggcggagg gggacgttta gggccgcctt ctagaacagc ccgatcactt
     2881 cccccttctc gccgaggtct atgcgctccg aggccggcga cttgggcagc cctggcatgg
     2941 tcattatgtc cccgcacacg gcgacgacga actccgcacc ggccgagagc cgcacctcgc
     3001 gtatcgagag cacgtggccg gaaggggctc cccgcagcga ggggtccgtc gagaacgagt
     3061 actgggtctt ggcgatgcac accgggtagt gaccgtaccc atcttcctga aacttgcgga
     3121 tctgacgccg caccttcgtg tccgccacca cctcgctggc gccgtagatc ttggtcgcca
     3181 cggcggagat cttatcccac aggctgtctt cctcgtcgta gacgaacctg aacctggctg
     3241 gcacgctctg tacggtctcc acgacggcgt gggccagcgc ttcggccccc acgccccctt
     3301 cggcccagtg gttcgtgagc acgacccgcg ccccctggtg agcgacaccg tctttgagga
     3361 gcgagatctc cgcctccgtg tccgacggga agcggttgat cgccacgacg cacgggagcc
     3421 cgaagtggtt gcggacgttg ttgatgtggc gttccaggtt cgccagcccg ccttcgagcg
     3481 ccccgagatc ctccttctct agctcgccgg gttcggcgcc gccgtggaac ttgagcgccc
     3541 tcatggtcgc cacgagcacg accatcgagg ggcgcaaccc ggacatccgg cactttatgt
     3601 ccacgaactt ctccgccccg agatcggcgc cgaaaccggc ctccgtgagg acgtagtccg
     3661 cgagcttgag cgccgttttc gtcgccatga cggagttgca accatgggcg atgttggcga
     3721 acggcccgcc gtgtacgaag gcggggttgc cctccagggt ctgcacgagg ttgggtttta
     3781 tggcgtcttt caagagcacg gccatagcgc cgtgggcgtc aagatcccgc gcaaccaccg
     3841 gcctttcgtc ccgcgtgtat cctacgacga tgtttcccaa ccgctccttc aagtcctcta
     3901 gtgaggtcgc gaggcagagt atggccatca cctccgaggc gaccacgatg tcgaaggcgt
     3961 cttctcgtgg aaaaccgttg gtcggccccc ccaaccccac gactatgtcg cgcagcgccc
     4021 tatcgttcat gtccatgacg cgcttccaca ctatgcgacg cagatccaac cccagatcgt
     4081 tgccctgatg aacgtggttg tccaccagag cggcgaggag gttgttggcc aaggctatgg
     4141 cgttgaagtc gcc
//
LOCUS       DATMVF010000053        11849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_328960, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000053 DATMVF010000000
VERSION     DATMVF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11849
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328960"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..850
                     /locus_tag="VN178_02365"
     CDS             77..850
                     /locus_tag="VN178_02365"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HWS79841.1"
                     /translation="MQIDFNCDMGESFGPYSFGADEELMRHISSANVAGGFHAGDPHV
                     MRETVALAREHGVAVGLHNGYGDLVGFGRREIKAAPEELRDELIYQLGALREFARLYG
                     LEIQHVKPHGALYMAAARDEELSRAIIGAIQESDPALVLYCMQSSKTHEIAQQMGQPV
                     AREFFADREYNDEGQIVFTRRVGEELDPEEVAERVLRGVTEGRVRTVDGNDIEVAADS
                     ICVHSDTPGAARLAETIVRMLGENGVEVRPVGRAEQRIP"
     gene            868..1104
                     /locus_tag="VN178_02370"
     CDS             868..1104
                     /locus_tag="VN178_02370"
                     /inference="COORDINATES: protein motif:HMM:NF005457.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HWS79842.1"
                     /translation="MANTVKAQMPGTFYRRPDPESDFYVEEGDRVSAGDTVGLIEVMK
                     SFHEVKAGEEGTIARFLVENEEAVDAGQDLLEME"
     gene            1196..3760
                     /locus_tag="VN178_02375"
     CDS             1196..3760
                     /locus_tag="VN178_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWS79843.1"
                     /translation="MTETARRYMGNSVPRKEDPTLLTGHSNFTDDISLPGMLHMAFLR
                     SPHAHAKITSIDVSAAREQPGVEAIFTSGDLADVLEGSAMDTDSAPGYAVEIDEPAGS
                     EADVTPTGNEESADISESPPRTFCGWPVTENIKIPHHSPLAHDEVNFAGEPVAVVLAT
                     DRYKAQDALEFIEVEYEPMAAATDIEGALEGGAPLVHEEFGTNECYLWPLATGDIDEA
                     FEKADVVVSGRYIQQRLIPSAIETRAVVAQPEPLDSFTVYTSTQVPHFVKDVLSVMCG
                     VPDTKLRVVAPDVGGGFGSKLNVYSEEVLALEVARKLGTPVKWVEDRSENHLATTHGR
                     GQVQHIELAATSEGKILGMKVKLLADMGAYLQLLTPGVAIFGSFTYPGLYAFDAYSFE
                     CTGVFTNLTPTDAYRGAGRSEAAYAHERIMDALARELDMDPAEVRLKNLIPPFDEPTT
                     NPAGVQYDSGDYESCMRKALELADYEEVRAEQRRRREAGDARQIGIGIGNFTESGGLS
                     PSKVSAAVRLQSGGWEAASVRMLASGKVEVVTGTSPHGQGHVTSWSQIAADALGVDVD
                     HVEVLHGDTAIAPYGRDTYGSRSLPVGGVAVHLACDKVVKKGKKIAAHMLEAAEEDIE
                     FEGGSFSVAGSPDQNVTIQDVAAAAYLADNLPEGMEPLLNEDYTFDPPNFTWPYGTHI
                     CVVEVDTETGMVTIPKYVAVDDCGPVVNPAIVDGQLHGGIAQGIAQALYEEAVYDDDG
                     NLVTGTMVDYLVPGAPEIPNMILDRTETPSPTNPMGVKGIGESGAIAAQPAVINAVID
                     ALSHEGVTHVDMPASPMRVWQTLQEARGQSAGTRDADLSGRPSMSSRAEQDDVDGV"
     gene            3878..4060
                     /locus_tag="VN178_02380"
     CDS             3878..4060
                     /locus_tag="VN178_02380"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWS79844.1"
                     /translation="MGRRSGRQDKDEGAMDRAKGQMKEAAGALTDDKDTKARGRADQR
                     RGKMKEKKGNLKDLLK"
     gene            complement(4204..4698)
                     /locus_tag="VN178_02385"
     CDS             complement(4204..4698)
                     /locus_tag="VN178_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79845.1"
                     /translation="METTERINRTAERLARTSADSYKMVIDHMVAQQERNVRFARETF
                     GDAVREIRHQAESNKVLTQELVERAETQRDAFRTLVGESVDAYTDLLYAPLAYYKQGL
                     RLVESEGFPIAGYDELNVREIGDRIDGMTAAEIRTVREYEKRNKNRETLIEQFDRKLK
                     AASA"
     gene            4999..5715
                     /locus_tag="VN178_02390"
     CDS             4999..5715
                     /locus_tag="VN178_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWS79846.1"
                     /translation="MCSRNLVLMLAALSLVVTLAACSSSGQSSDEGGKDQAGKDEQAQ
                     KQRPSEKAAGGKKEPSREEPPKMTKEAKGKQVAPTKGKGQRKVANRLEPPKDKTLKLT
                     IPEMKQIEDDTIPTGKGTNEALFRDHAAVHLRDTGFPWQRTANVYIAGHRIGFPGTDS
                     NLAFYDLEDLKDGDKVYLEDAEGREYTYEVFGKLVVEPANLSVLRPIKGKNIVSLQTC
                     TLPDYTNRVIYRAVLRDVKT"
     gene            5831..6703
                     /locus_tag="VN178_02395"
     CDS             5831..6703
                     /locus_tag="VN178_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWS79847.1"
                     /translation="MMALSKDGGPAFGAHAFIWAGEWTREGAERVISGAADAGLDFVE
                     IPLLDPGSIDVEHTRSLLDRYGLGCSCSLGLPPEVHLPSNPEGARRFLDGAVEATAAL
                     GSPILTGCLYTHVGTLTGKPPEPAEIELVVRVLKSTARRAAEYGVELGIEAINRYETY
                     LLNLASQAGELLDRIDEPNVFVHLDTYHMNIEEKGFYEPIVSLGSRLGYIHLSESDRG
                     TPGTANVHWEEVFRGLGDIDYGGALAMESFAAVNEALIGATALWRDVVGDPDALVRDG
                     LAFLRARGREQGVL"
     gene            6748..8199
                     /gene="xylB"
                     /locus_tag="VN178_02400"
     CDS             6748..8199
                     /gene="xylB"
                     /locus_tag="VN178_02400"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HWS79848.1"
                     /translation="MLLGLDLGTSSVKALLMAEGGAVIGEGSASYPVSAPRPGWAEST
                     PEDWWGGVLEAAGAAVGRRGAEVTAIGLSGQMHGVVLVDEPGIPLRPAVIWADARSGA
                     ELAAYRRLDEDLGRRLANPPAVGMAGSSLLWLRDHEPEAYTSARWALQPKDWLRMRMT
                     GEAATEPSDASATLLYDLPADDWSYAVVEDLGLRTELLAPFVPSARVAGTLVGEAAGE
                     LGLREGLPVAAGAADTAAAMVGTGLLRPGPVQLTIGTGGQVVTPKRVPEPDPLGRTHL
                     YRAALPGLWYSMAAVQNAGLALEWVRRVLGVSWKEVYEEAFAVPQGSGGVTFLPYLSG
                     ERTPRFDPGARGAWTGLGLDHTRGHLLRAALEGVAFALREALEALEDQGITAPELRLA
                     GGGTGGGSGEPWRQLLADVLGRPLWQLPDEISSVASARGAAFLAGLASGIYPTAEDTL
                     PLTPEPESVIRPGGPAYETAYERYKNLYPKLHG"
     gene            8366..9793
                     /locus_tag="VN178_02405"
     CDS             8366..9793
                     /locus_tag="VN178_02405"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HWS79849.1"
                     /translation="MLSREYTGPLEENGTSPGVRWLLADRVGRALSDARLESLADPVE
                     RIVRLAVPAGRVDPFRWLSEQRMFPKVYWSGREDRAGVAAVGIADLREASASEGPRSL
                     SKALASLPDSGVSGARYYGGARFDPLGQPDEEWAAFGAYRFVLPRFELHAGETETTLV
                     CNLVLPRDTDDASKITQEIEDLSLPEGGYGAPLPAPVLREDSPDLRGWRGNVERALAA
                     FSEGSLGKVVLARRAEFGFCGDLDPTLLLESLKAATPGCFHFYAEPESGTAFLGASPE
                     RLFRRDGRMVESEAVAGTRPRGVSSAEDEGLRDALLHSAKDRVEHTYVSKGIREALLP
                     LCEELEIEDHVSEMKLVRGRHLRSKVRGTLRDGVTDAALLDAMHPTPAVGGHPRTGAL
                     EQIQALETFDRGWYAGPVGWIGAEGSEFAVGIRSGLVRGKTLALYSGAGIVAGSVPDE
                     EWAEIEQKIGDFTGMFGLEQGHAAS"
     gene            9768..11513
                     /gene="menD"
                     /locus_tag="VN178_02410"
     CDS             9768..11513
                     /gene="menD"
                     /locus_tag="VN178_02410"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HWS79850.1"
                     /translation="MNRDTPRANRLWAYLIVEELLRCGVDFFCVAPGSRSTPLVAALA
                     ANERARSLIHFDERGTAFAALGYARATGRAAAWITTSGTAVANGLPAVVEAATDGVPM
                     ILLTADRPPELRQTGANQTIDQPDIFGDYVRWRFDLPAPDPAIDPAMVLTTVDQAAYR
                     AGRTPQGPVHLNLMFREPLLPDPGDEPVLSGPSSWVEGDRPYTRYAATKPAVDEMEVH
                     RLWETLHPVRRGLVVAGRLASRKQGEAVLRLAETLGWPLLPDIGSQVRLGSEGLTAHY
                     DALVAGASFAGSHAPEAVVHLGGRALSKRLEQFLAGSRPDPYVVVRENPFRLDPAHRV
                     THSVEANVLDFCSALARAAAEDPPAMDSSWTAGWLKASEEVGRHLDGDLSGEPNEPLV
                     ARVLSQNAPPDHGLVVASSMPVRDLDTYATTNGAPVPVAANRGASGIDGTVATAAGFA
                     RGLGRPVTLLIGDLALLHDMNSLAMLRDVHVVVVVLNNDGGGIFSFLPIARHEGFFET
                     YFGTPQGVGFEPAAKMFGLAYEHPGTMEEFVEAYGSACARGSSTLIEVETDRGENVTL
                     HRRLLQEVADRVARL"
     gene            11513..>11849
                     /locus_tag="VN178_02415"
     CDS             11513..>11849
                     /locus_tag="VN178_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWS79851.1"
                     /translation="MGVVLNYEVAGDRRSPAVLFLHGFMGSSVDWREVIAAPGDRFCV
                     AVDLPGHGASLGLPSDTYTIEGAIRAVIRTLDDLQIFRPVVAGYSMGGRLALYLALRY
                     PERCAGLFLE"
BASE COUNT         2175 a         3728 c         3951 g         1995 t
ORIGIN      
        1 tgcgctggcc ggaaagagcc gaggagaagg aagctgacag ctgaatgctg acagcctgcg
       61 accaaaggga gcagaaatgc agatagactt caattgcgac atgggcgaga gcttcggccc
      121 ttacagtttc ggtgcggatg aggagttgat gcgccacatc tcctcggcca acgtcgccgg
      181 cggctttcac gctggtgacc cgcatgtcat gcgggagacg gtggcgttgg ccagagagca
      241 cggcgtcgcg gtcgggttac acaacgggta tggtgatctg gtcggcttcg gcaggaggga
      301 gataaaggcc gcgcctgagg aactccgcga cgagttgatc taccagctag gtgcgctgcg
      361 cgagttcgcc cgcctctatg gcctggagat ccagcacgtc aagccccacg gcgccctcta
      421 catggccgca gcccgcgacg aggagctctc gcgtgcgatc atcggggcga tccaggagtc
      481 agacccggcc ctcgtcctgt actgcatgca atcctcgaag acccacgaga tcgcccagca
      541 gatgggccag cccgtcgcga gggagttctt cgccgaccgc gagtacaacg acgaaggcca
      601 gatcgtcttt acccgtcgcg taggcgagga gctagatccg gaggaggtgg ccgagagagt
      661 cctccggggc gtgacggagg gcagggtcag gaccgtggac ggtaacgaca tagaggtggc
      721 tgcagactcg atctgcgtcc acagtgacac cccaggggcc gcgaggctgg ccgagaccat
      781 agtgcgcatg ctcggggaga acggcgttga ggtaaggccc gtcggacggg cggagcagag
      841 gattccctag cgcgaggaga gttcgagttg gcaaacacag tgaaagccca gatgccaggt
      901 accttctacc gccgccccga tccggagagc gacttttacg tcgaggaggg cgaccgggtg
      961 tcggccggag ataccgtcgg cctgatcgag gtcatgaagt cgttccacga ggtcaaggcc
     1021 ggggaagagg gcaccatcgc caggtttctc gtcgaaaacg aggaggcagt ggacgcgggc
     1081 caggatctcc tagagatgga ataggatgcc gggatacgcc tcagtgatcc acggtgcacg
     1141 atagaagtgg ctgacactgg ttaaaggact ccgactcgaa ggaggagatt gcgccatgac
     1201 ggaaacggcc cggcggtaca tgggcaacag cgtaccacgc aaggaggacc cgacgctcct
     1261 tacgggacac agcaacttca ccgacgacat cagcctgccg gggatgctgc acatggcctt
     1321 tctacgcagc ccccacgccc acgccaagat cacctccatc gacgtctctg ccgccaggga
     1381 gcaacccggc gtcgaggcca tctttaccag cggggacctg gccgacgtgt tggaaggcag
     1441 cgcgatggat accgacagcg cgcccggcta cgccgtcgag atcgacgaac cggccgggag
     1501 cgaggccgat gtcaccccaa ccggtaatga agagtccgcc gacatatccg agtctccacc
     1561 gaggaccttc tgcggctggc cggtcacgga gaacatcaag atcccgcacc actcgcccct
     1621 ggcccacgac gaggtcaact tcgccgggga gccggtagcg gtcgttttgg cgacagaccg
     1681 ctacaaggcc caagacgccc tcgagttcat cgaggtcgaa tacgagccta tggctgcggc
     1741 gacggacatc gagggtgcgc tcgaaggcgg ggctcccctg gtgcacgaag agttcgggac
     1801 caacgagtgc tacctctggc cgctcgcgac gggagacata gacgaggcct tcgagaaggc
     1861 ggatgtggtg gtcagtggac gctacatcca gcagcgcctc atccccagcg ccatcgagac
     1921 gcgggccgtc gtggcacagc ccgagccgct ggatagcttt accgtgtaca cctcgacgca
     1981 ggttccccac ttcgtcaagg acgtgctctc cgtgatgtgc ggtgtgcctg acaccaagct
     2041 gcgggtcgtg gcgccggacg tgggaggggg tttcggttcc aagctcaacg tctactccga
     2101 agaggtgctc gcgctggagg tggcgcgcaa gctcgggacg ccggtgaagt gggtggagga
     2161 ccgctccgag aaccacctgg cgacgaccca cggtcgggga caggtccagc atatcgagct
     2221 cgccgcgacg agcgagggca agatcctcgg gatgaaggtc aagctcctgg ccgatatggg
     2281 cgcctacctg cagctcctaa cacctggtgt cgccatcttc ggatccttca cttaccccgg
     2341 cctctacgcc ttcgacgctt actccttcga gtgcaccggt gtctttacca acctcacgcc
     2401 gaccgacgcc taccgtggcg ccgggcgctc cgaggcggcc tacgcccacg agcgcatcat
     2461 ggacgccctc gcccgggagc tcgacatgga cccggcggaa gtgcgcctga agaacctgat
     2521 tccgcccttc gacgaaccca ccacgaaccc ggcgggcgtc cagtacgact caggcgacta
     2581 cgagtcctgc atgaggaagg ccctggagct ggctgactac gaggaggtcc gtgccgagca
     2641 acgtcgccgc cgcgaggccg gtgacgccag gcagatcggc atcggcatcg gcaatttcac
     2701 cgagagcggc ggtctctcac cctcgaaggt ctcggcggcc gtccgcctgc agagcggagg
     2761 ctgggaggca gcctccgtgc gtatgctcgc ctcgggcaag gtggaggtcg tcacgggcac
     2821 cagcccacac ggccagggcc atgtcaccag ctggtcccag atagccgccg acgccctggg
     2881 cgtcgatgtg gaccacgtcg aggtcctgca cggcgacacg gcgatagctc cttacggccg
     2941 cgacacctac ggctcccgca gcctcccggt cgggggcgtc gcggtgcacc tcgcctgcga
     3001 caaggtggta aagaagggca agaagatagc ggcgcacatg ctggaggcgg cggaggagga
     3061 catcgagttc gagggcggaa gcttctccgt ggccggttcc cccgaccaga acgtcacgat
     3121 acaggacgtc gccgctgcgg cttacctggc cgacaacctc cccgagggga tggaaccgtt
     3181 gctaaacgag gactacacct tcgatccgcc caacttcacc tggccgtacg ggacccacat
     3241 ctgcgtggtc gaggtggaca cggagacggg gatggtgacc atccccaagt acgtggcggt
     3301 ggacgactgc ggacccgtag tcaacccggc tatcgtggac ggccagttgc acggtggcat
     3361 cgcgcagggg attgcgcagg ccctctacga ggaggccgtc tacgacgacg atggcaacct
     3421 ggtgaccggc acgatggtcg actacctcgt ccccggcgcc cccgagatac cgaacatgat
     3481 cctcgaccgg acagagactc cctcccccac caacccgatg ggtgtgaaag gcatagggga
     3541 gtcgggcgcc atagccgccc aaccggcggt catcaacgcg gttatagatg cgctctccca
     3601 cgaaggcgtc acccacgtgg acatgccggc ttctccgatg cgggtgtggc agaccttgca
     3661 ggaagcccga ggccagagtg ccggtacgcg cgacgccgac ctctcaggac gcccttcgat
     3721 gtcgagtagg gcggagcaag acgatgtcga tggtgtctag cccgaacacg gtgtaagtcc
     3781 atccccggtc tccggcggaa aagctaccgg caggcgtttg attccagcac ctccgggtaa
     3841 gtaaaccaat gaacattccc gggatgagga ggaacaggtg ggtagacgga gcggtcggca
     3901 ggacaaagac gaaggcgcca tggatagggc aaagggccag atgaaggaag cggcaggcgc
     3961 cctgaccgat gacaaagaca cgaaagccag gggccgtgcg gaccagcgcc gcggaaagat
     4021 gaaggagaag aagggtaatc tgaaagactt gctgaagtag cttgtaaggc ttcaggcttc
     4081 aggaaaaagt cggttggtag gcgaaagggc cgggagagaa tccccggccc tctcttttgt
     4141 cagccgcgtt gtgtccgcgc cagtttgtcg gctcacaacg ccctgaaacc tgtaacctga
     4201 agcctatgca gaagcggcct tgagcttgcg gtcgaactgc tcgatcaggg tctcgcgatt
     4261 cttgttccgc ttctcgtact cgcggaccgt gcggatctcc gccgcggtca tgccgtcgat
     4321 gcggtcaccg atctccctga cgttcaactc gtcgtagcca gcgatcggaa acccttcgct
     4381 ctcgacgagg cgcagaccct gcttgtagta ggctaggggc gcgtatagca gatccgtgta
     4441 agcgtcgacc gactcgccaa ccagcgtccg gaaagcgtca cgctgcgtct cggcccgctc
     4501 gacgagctcc tgggtcagca ccttgttgct ctcagcctga tgccggatct cacgcacagc
     4561 gtcgccgaat gtctcccgcg cgaaccgcac gttgcgctcc tgctgagcta ccatatgatc
     4621 tatgaccatc ttgtaggagt cggcgctcgt cctcgccagc cgctccgcag tcctgttgat
     4681 ccgctctgta gtctccactc tctgcctcct cttaatggat atcatctatg tgcagtatac
     4741 acatatattc aagcacgtca agatcgaagt ttatggcggg cgagaatcgg aggtttccat
     4801 ggtgctggat cctggccttt acacccggca ttgcaaacct taatatgacc gtccggcagg
     4861 gtgatccagg cgtgtattga gaggtctctt gcggtgagac ttgtaacgaa tgtttggtct
     4921 aactgttacg gccttgttat cctggatctg tatgctctct gagcacggcg tctggtaaag
     4981 acagcgagag gtgatgagat gtgttctaga aatctggtgc tgatgttggc ggcactgtcg
     5041 ctggtggtga cgctcgctgc ttgctcttcc tcgggccaat cctccgatga gggcgggaag
     5101 gatcaggcgg ggaaagacga gcaggcgcag aagcagagac cttccgagaa ggcggccggg
     5161 ggcaagaagg agccttccag ggaggagcct cccaagatga cgaaggaggc gaagggcaag
     5221 caggtcgcgc cgacgaaggg taaggggcag cgtaaggtag ccaacaggct cgagcccccc
     5281 aaggacaaga cactcaagct cacgattccc gagatgaagc agatcgagga cgacacgata
     5341 ccgacgggta agggcacgaa cgaggcgctg ttccgcgacc acgctgctgt ccatctgagg
     5401 gacacgggtt tcccgtggca gaggactgcc aacgtctaca tcgcaggaca ccgcatcggc
     5461 ttccctggga cggacagcaa cctggccttc tacgatctcg aggacctgaa ggacggcgac
     5521 aaggtctacc tcgaggacgc agaaggcaga gagtacactt acgaggtctt cggcaaactc
     5581 gtcgtcgagc ccgcgaatct ctccgtactg aggccgatca agggcaagaa catcgtcagc
     5641 ctgcagacct gcacgctacc cgactacacg aaccgtgtca tctacagggc cgtgctcagg
     5701 gacgtcaaga cgtaagccac agggcggata cagagcgatg aacccacagt aggaagcggg
     5761 gtcccgccgg cgtgttgtcg gggctacggc ctgtccgagc aggggtcggg gaacacagaa
     5821 cggaggagag atgatggcgc tctcaaagga cggtggtccg gccttcgggg cccacgcctt
     5881 tatctgggcg ggtgagtgga cgcgcgaggg tgcggagcgt gtgatctcgg gcgccgccga
     5941 tgctggcctg gacttcgtgg agataccgct cctcgatccc ggctccatag acgtggagca
     6001 tacccgttcg ctgttggatc gctacggtct cggttgctcg tgctcgctcg gccttcctcc
     6061 tgaggtccat ctgccctcca accccgaagg cgcgcggcgg ttcctcgacg gggctgtgga
     6121 ggccacggca gccctgggct cgccgatact cacgggttgt ctgtacacgc acgtcggcac
     6181 gctgacgggc aagccccccg agcctgcgga gatcgagctc gtcgtacgcg tactcaagag
     6241 tacggcccgt cgcgccgcgg agtacggcgt cgagcttggt atcgaggcca taaatcgcta
     6301 cgagacatac ctcctcaacc tcgcttcgca ggccggggag ctgttggatc ggatagacga
     6361 gcccaacgtt ttcgtccacc tcgacacata ccacatgaac atagaagaga agggcttcta
     6421 cgagccgatc gtctctctgg gatcccggct cggctacatc cacctttcag agtccgaccg
     6481 aggcacgcca gggacggcca acgttcactg ggaggaggtg ttccgcggcc tgggggacat
     6541 cgactacggc ggcgcgctgg cgatggaatc attcgccgcc gtgaacgagg cgctcatcgg
     6601 cgcgacggcg ctgtggcgcg acgtggtcgg tgaccccgac gccctcgttc gggacgggct
     6661 cgccttcctg cgcgcgaggg gacgagaaca aggtgtgctc tgatgaagga gaaccgttcg
     6721 tcccaagacc ggggataaga cgcaccgatg ctcctcggcc tcgacctggg aacgagttcc
     6781 gtcaaagcgc tgctaatggc cgagggcggc gccgttatcg gggagggttc cgcctcctac
     6841 ccggtgagcg cgccgcgtcc ggggtgggct gagtctactc cggaggactg gtgggggggc
     6901 gtactggaag cggcgggcgc cgccgtcggg cggcgtggag cggaggtgac ggcgatcggg
     6961 ctctctggcc agatgcacgg cgtcgtcctc gtggacgagc ctggcattcc cctacggccc
     7021 gccgtcatct gggccgatgc cagatccggt gcagagctgg cagcctaccg ccggctcgat
     7081 gaggatctgg gccgccggct cgcaaacccg ccggcggtgg gcatggcagg atcgagcctc
     7141 ctgtggctgc gggatcatga gcccgaggcc tatacatccg ccaggtgggc gctgcagccg
     7201 aaagattggc tgcgcatgcg tatgacgggg gaggccgcaa cggaaccgtc ggatgcctcg
     7261 gccaccctgc tctacgatct ccccgccgac gactggtcct acgcggtggt cgaggatctc
     7321 ggcctccgca cggagctcct ggccccgttt gtcccctccg cccgcgtcgc cggaacgttg
     7381 gtgggagagg ccgcgggcga actcgggtta cgggaggggc tgcccgtcgc ggcaggagcg
     7441 gccgacaccg ctgccgccat ggtcggcacc gggctgttac gcccgggtcc cgtgcaactc
     7501 acgatcggca cgggcggcca ggtcgtgacg ccgaagaggg tgcccgaacc cgacccgcta
     7561 ggtcggaccc acctgtaccg ggctgcctta ccagggctct ggtattcgat ggcggcggtc
     7621 cagaacgctg gcctcgccct ggagtgggtg cgcagagtac tgggcgtctc ctggaaagaa
     7681 gtttacgagg aggcgttcgc ggtgccgcaa ggctcgggag gcgtcacgtt cctgccttac
     7741 ttgagcggag agcggacgcc gcgcttcgac cccggcgccc gtggcgcctg gaccggcctc
     7801 ggcctcgacc acacgcgtgg ccacctcctg cgtgccgccc tcgaaggcgt cgccttcgcc
     7861 ctgcgtgagg cactcgaggc gctggaggat caggggatca ctgcccccga actgcgcctc
     7921 gccggaggtg gaaccggagg gggttcgggc gagccctggc gtcaactact ggccgatgtg
     7981 ctcggccgcc cgttgtggca gttgccagat gagatctcct ccgtggcatc ggcccgcggc
     8041 gccgctttcc tggccggcct cgcttccggt atctacccta cagcggagga cacactcccc
     8101 ctcacccccg aacccgagag cgtaatacga cccggcgggc cggcctacga gaccgcctac
     8161 gaacgctaca agaacctcta cccaaagctc cacggttgaa tactcgaaag tacagcgatc
     8221 agccatcagc gctcagcatt cggcaaggat gaaaagctta aggctgagag ctgaccgctt
     8281 acagctcgaa attgtgagcg aagccgctta actgctagat actccttgct acactacacc
     8341 ctgagatgac gaggcggcac atatcttgct gagcagggaa tacaccggtc cccttgagga
     8401 gaacggcacg tcaccgggcg tccgctggct actggccgac agggtgggcc gtgcgctctc
     8461 ggatgcaaga ctggaaagcc tcgcggaccc ggtcgagcga atcgtgcgtc tcgccgtacc
     8521 cgcgggccgg gtagaccctt tcaggtggct ctcagagcag cggatgttcc cgaaggtcta
     8581 ctggtcggga cgtgaagacc gggccggcgt cgcggccgtg gggatcgcgg acctgcggga
     8641 ggcgagcgcc tctgaggggc cgagatctct gtccaaggcg ctcgcgtcgc tgcccgactc
     8701 gggggtttct ggggctcgct attacggcgg agcacgtttc gatccgcttg gccagcccga
     8761 tgaggaatgg gctgcgttcg gcgcttaccg gttcgtcctg ccgcgcttcg agctgcacgc
     8821 gggggagacg gagacgacgc tggtttgcaa cctggtgctc cccagggata cggatgacgc
     8881 ctcgaagata actcaggaga tcgaagacct ctcgttaccc gagggtgggt acggtgctcc
     8941 gttgccggca ccggtactcc gtgaagacag ccccgacctg cggggttgga gggggaacgt
     9001 cgagcgggct ctcgcggcgt tttcggaggg aagtctgggg aaggtcgtgc tggcccgcag
     9061 agccgagttc ggtttctgtg gggacctgga tcccacgctc ctactggaga gcctgaaggc
     9121 ggctacgccg ggctgcttcc atttctacgc cgagcccgag agtgggacgg cattcctagg
     9181 ggcctccccc gaacgcctgt tccgccgcga cggacgcatg gtggagagcg aggccgtggc
     9241 ggggaccagg ccccgtggag tctcttcggc ggaggacgag ggtctgcgcg acgccctgtt
     9301 gcacagcgcc aaggaccggg tcgagcacac ttacgtgagc aaaggtataa gggaggcgct
     9361 gctgccgctc tgtgaagagc tggagatcga ggaccacgtc tccgagatga agctggttcg
     9421 gggaaggcac ctcaggtcga aggttcgtgg gacgctcagg gatggtgtga cggacgccgc
     9481 gctgctcgac gcgatgcatc ctacgccggc cgtcggcggg catccgcgaa ccggggcgct
     9541 ggagcagata caggccttag aaaccttcga ccggggctgg tacgccgggc ccgtgggttg
     9601 gatcggggcc gaaggctccg agttcgccgt tggtatacgt tcggggctgg tgcggggaaa
     9661 gacgctcgcc ctctattcgg gggccgggat agtcgccggc tccgtgcccg acgaggagtg
     9721 ggccgagatc gagcagaaga tcggcgactt cacggggatg ttcgggcttg aacagggaca
     9781 cgccgcgagc taatcgcctc tgggcgtacc tgatcgtcga agagcttctc cgctgcggcg
     9841 tagacttttt ctgcgtcgcc cccggctcgc gctcgacgcc cctggtcgct gcactagccg
     9901 cgaacgagag ggcccgtagc ctcatacact tcgacgagcg cgggaccgcc ttcgccgccc
     9961 tcggctacgc ccgcgccacg gggcgtgctg cggcctggat cacgacctcg ggcaccgcag
    10021 tcgccaacgg ccttccggcc gtcgtggagg ccgcgaccga cggcgttccc atgattctgc
    10081 ttaccgccga tcgtcctccc gagctccgcc agaccggtgc caaccagacc atagatcagc
    10141 ccgacatatt cggcgattac gtccgctggc gtttcgatct gccggccccc gaccccgcca
    10201 tcgacccggc catggtcctg acgaccgtgg atcaggctgc ctaccgtgcg ggccgaacgc
    10261 cacagggccc agtacacctg aacctgatgt tccgcgagcc cctactcccc gatccggggg
    10321 acgagcccgt gctttccgga ccatcctcct gggtagaggg cgatagaccg tacacccgct
    10381 acgccgcgac gaaacccgcc gtggacgaga tggaggtcca ccgcctctgg gagacgctcc
    10441 accccgtaag aaggggtctc gtcgtcgccg gcaggctggc ctcccgcaag cagggcgagg
    10501 cggtcctgcg gctcgccgag accctgggct ggccgttact acccgacata ggctcccagg
    10561 tccgactcgg ttcagagggc ctcaccgccc attacgacgc gcttgtggcc ggggcctcgt
    10621 tcgctggatc acacgcaccg gaggctgtag tccacctcgg aggaagggcc ctttccaaac
    10681 gcctggagca gttccttgcc ggcagccgcc ccgaccctta cgtcgtcgta cgcgagaacc
    10741 ctttcaggct ggatcccgcc caccgcgtta cccacagcgt cgaggcgaac gtcctcgact
    10801 tctgctccgc attggcccgg gccgctgccg aggacccacc ggcgatggac tcttcctgga
    10861 cggcgggctg gcttaaggca tcggaggagg tggggcgcca tcttgatgga gacctctccg
    10921 gagaaccgaa cgagccgctc gtggccaggg tcctctcgca aaacgcaccc ccggaccacg
    10981 gtctcgtcgt cgccagcagc atgcccgttc gggacctgga tacctacgcg acgactaacg
    11041 gcgcgcccgt tcctgtcgcg gccaacaggg gcgcgagcgg tatcgacggc accgtcgcca
    11101 cggccgcagg cttcgcgagg ggcctcggac ggcccgttac gcttcttatc ggggatctcg
    11161 cgcttttgca cgacatgaac tctctcgcca tgctgcggga cgtacacgtc gtcgtcgtgg
    11221 tgctgaacaa cgacgggggc ggtatcttct ccttcctgcc catcgccagg cacgaggggt
    11281 tcttcgagac ctacttcggc acgccgcagg gcgtgggctt cgagccagcg gcgaagatgt
    11341 tcggactcgc ctacgagcac cccggcacga tggaggagtt cgtcgaagcg tacggttctg
    11401 cgtgtgcccg cggctcgtcg accctgatcg aagtagagac cgaccgcggg gagaacgtga
    11461 cgttgcaccg caggctgctc caagaagtgg cggaccgggt tgccaggttg taatgggtgt
    11521 cgttctgaac tacgaggtcg cgggagaccg ccggtctccc gcggtcctct tcctgcacgg
    11581 tttcatgggt tcttccgtcg actggcgaga ggtgattgcg gcgcccgggg atcgattctg
    11641 cgtagccgtg gacctgcccg ggcacggcgc atcgctgggg ctgccctctg atacgtacac
    11701 gatcgagggc gccatacggg ctgtgatccg aaccctggac gacctccaga tcttccgtcc
    11761 cgtagtcgcc ggttactcga tgggcgggcg gctggctttg tatctggctc tgcgttaccc
    11821 ggaacggtgt gcggggctct tcctcgaat
//
LOCUS       DATMVF010000054        14956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_509853, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000054 DATMVF010000000
VERSION     DATMVF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14956
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509853"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VN178_02420"
     CDS             <1..496
                     /locus_tag="VN178_02420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWS79852.1"
                     /translation="TEKEQTLNVAKRLDTLLEGDGYTVCMTRTSNSQTLSNNARYTYA
                     NTTGADVLVSIHMNGSTNGSTDYTTTLFGKWRKDKELANTIFGSLSTLPAASGTGTIG
                     TRTPYSFASGVLLKSNMPATIAETVFITSDREGQLLSNGTGARQQQIAQALRVGIEEY
                     LATH"
     gene            557..1462
                     /locus_tag="VN178_02425"
     CDS             557..1462
                     /locus_tag="VN178_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS79853.1"
                     /translation="MSLAIETEKLTKAYGRRARGIEDVDLAVEEGEVFGFLGPNGAGK
                     TTTVRTLLGFLRPTGGRGEVFGLDITKDSVEIRARVGNLPGEFALEDKMTGEGLLKYF
                     ARLRGVGDLGYAHEISERLGAELHRPMRRLSRGNKQKIGLVQAMFHRPPLLMLDEPTG
                     GLDPLVQEEFLDIIEEVKAEGRTVFFSSHNLAEVERACDRVGIIRGGRLAAVETTETL
                     IDKSFRHVVLTFAEDVDREPFANLSGVRNLRGDGKRISFTLYDDPDEMVKLAAKHTLV
                     NMEYERPSLEEVFLTYYEQKNGEVR"
     gene            1459..2304
                     /locus_tag="VN178_02430"
     CDS             1459..2304
                     /locus_tag="VN178_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWS79854.1"
                     /translation="MSVVLDRGQKRRRAARPGRTLFALVTHTVRMQLRGVIIWGAAIG
                     LYGAAMVASFTAIEGSAVQLNQLMEAYPKGMLEAFGVTDLGDPANYLHSQVFGLAPLA
                     LSFFPILALANALAGAEERGTIDVLLGNPLPRWQLVAGNFLAGAVSLLGICAIVGLLT
                     WGTAVLVDVELSRREVAEAVLNLWPICIVFGALALLCSASFHRRSLAIAIPAFLLFGM
                     YLIDTIGRVSEDLEDWRPFSVFYYYGSAIENGIDWIHFGSITLVALALLLLATLVFRR
                     RDIYT"
     gene            2508..3803
                     /locus_tag="VN178_02435"
     CDS             2508..3803
                     /locus_tag="VN178_02435"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS79855.1"
                     /translation="MERAETKALLIRARGIATFRGVLDSAVARDLLGLLELLEESPDA
                     GAVARIFGRLWEGLALEEERLLPDAWQSHLVGRVLDDENPFSLGAEKGEADSSVLGQA
                     RRDLRTLGEMFAMDAAELLGRVESVVPALAGIWVPWRSPEPVEESPRRTIARKLSAAE
                     DWGACAELLAGHFARHGAGPYGRHRAFLWREGRLLAVSRPDPVSLSDLITYEREREPL
                     IRNTERFLAGLPAHHALLYGLPGTGKSSTVKAIMNEYADLGLRLVEVKKEDLAELPGV
                     LEVLGGRGRRFVLFVDDLSFEEHEVEYKALKALLEGSVEEPPENVRLYATSNRRNLIR
                     ETFSERDDGTTGDDVHARDTMQEKLSLAARFGLRLTFPSPDQARYLKIVSGLARERGL
                     AIPAEDLRERALLWDRWHAGRSGRTARQFVDELEAEEQS"
     gene            complement(4024..4581)
                     /locus_tag="VN178_02440"
     CDS             complement(4024..4581)
                     /locus_tag="VN178_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWS79856.1"
                     /translation="MSEAPAAWQGPKAERDAVPIHQSARDNKDAQALQRQARDEMSNK
                     VILLVEDNPDDELLTLRALKKSGVSNEVVVAHDGVEALDYLFASGTYAGRDPAVKPQL
                     ILLDLKLPRVDGLEVLKRLRSDERTRRLPVVILTSSNEQRDMLDGYGLGANSYVRKPV
                     DFEQFINAVEQLRLYWLVLNEAPPD"
     gene            complement(4574..5023)
                     /locus_tag="VN178_02445"
     CDS             complement(4574..5023)
                     /locus_tag="VN178_02445"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWS79857.1"
                     /translation="MQQDPNFGSERERGSGEQVGASMDTMTAQDERTWSIIAHLSVLL
                     GLVGLMPFGALLVWLIYKDRSQKVRFHALQALWYQIAWILILVVYTLVSLVLSLVIIG
                     IFMFFLLPILALVPLVHGCYAAYQVSKGVEYRYPYIADRIEGPRGVV"
     gene            5301..6725
                     /locus_tag="VN178_02450"
     CDS             5301..6725
                     /locus_tag="VN178_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWS79858.1"
                     /translation="MGIPLSVLLVEDSENDALLLLRMLRRGGYDPTWERVETAAAMEA
                     ALDGRSWDLVISDHSLPTFSSLAALGLLRRKGFVDLPFIIVSGRIGEDAAVSAMKAGV
                     HDYLMKDNLARLNSAIERELRDAKVRRERRQAEEKYRSIFENAVEGIFQTTVDGRFLT
                     ANPAMARMLGYESPEELLGAISNIRDQLYVEPGRREEFYQLALRDGFVSGFEVQMHRK
                     DGDLLWISANARAIYDNHGEISGYEGTVENITERKRAEEALREIREAERRRIARELHD
                     VVLQDLTYALQSMQVLRRLPEGVDRAEEMGRQVEALKRAVGGLRDAIYDLRLESARER
                     PLVRAVESIVELNRQIGEGCQFEMIVEDGFPAAISGPAGVEVVRVVQEALTNVRRHSG
                     AGRAVVTLGRVNGEILVEIEDDGRGFGPETSYGMGLTGMRERVLALGGELEVEGREGV
                     GTRVFLRVRMGVLAGGETGERSNT"
     gene            6805..7458
                     /locus_tag="VN178_02455"
     CDS             6805..7458
                     /locus_tag="VN178_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS79859.1"
                     /translation="MRRILIVEDHASFRQTLAFVFDQETEFEVVAQAGTLAEARRTMD
                     DSEADLGIIDLSLPDGEGTDLIEELREANPDFAALVLTASMDRMEYARAVEAGAAGVL
                     HKSADVDSILDATRRVGEGEALLSDDELISLLRLAGQNREEEVEARASIEQITPREME
                     VLQMLASGLSNKEIAAELHMSVDTERTHMMNILNKLGVHSRLQALVFAARHGLVKIR"
     gene            complement(7566..8351)
                     /gene="dapF"
                     /locus_tag="VN178_02460"
     CDS             complement(7566..8351)
                     /gene="dapF"
                     /locus_tag="VN178_02460"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWS79860.1"
                     /translation="MRFTKMEGCGNDFLIFDPGQVEGRNLADLALMACDRHFGVGADG
                     ILIPAPSETADLEMIYLNSDGSTSEMCGNGLRCLVRYAKDRGMVGGDAMTVATGAGVK
                     KVLLFDDGSSRVDMGAPHVDTEVESDGFHFLRVSMGNPHAVAFLESEAAVDALDLATV
                     GPPIEVDPIFPEKTNVEFGFARGGHDVRMRIWERGAGETLASGSGSCAAAVAAVSKNL
                     ARSPVSVYMDGGAVQIEWSGHGRPVYIEGPAEYVCEGELVLRS"
     gene            8438..8719
                     /locus_tag="VN178_02465"
     CDS             8438..8719
                     /locus_tag="VN178_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWS79861.1"
                     /translation="MRSNRAKYRRRRIGALFVGAALAVAVYAGGGAGAEPRPVHYTVA
                     PGDTLWGIALEHTPIWEDPRPKVEAIREANDLSGYEIYPGMSLKVPSTV"
     gene            8971..9153
                     /locus_tag="VN178_02470"
     CDS             8971..9153
                     /locus_tag="VN178_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79862.1"
                     /translation="MEDRGEVCLAEEVDRFRRLGLSDGEIASEMGVDLSWVETVVSSG
                     EDESSPDAEDRAVDET"
     gene            complement(9217..11082)
                     /locus_tag="VN178_02475"
     CDS             complement(9217..11082)
                     /locus_tag="VN178_02475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWS79863.1"
                     /translation="MTDASTVPLIPREVLFGNPEKMAPHLSPDGERLAYLAPKDGVLN
                     VWVGPVGSSAGGEDFEPVTDDRKRGIRVYFWAEDNTHIVYLQDEGGDENWRVHAVDAS
                     TKQDRDLTPFDEVQAQIVDSNRRFPDTLLVALNRENPELHDVYRLTLSTGDLELVARN
                     PGNAVGWVVDPQLEVRGAMAATPDGGFELLLREGTGEDAGWRKLLGWGKEDALSSAPV
                     GFTEDGDKMYLIDSRGANASRLVLLDLEDDSTQTLVEDPRYDIGRVVTHPETHEAQAA
                     AVERARTEWIVLDDDIREDFEVIDGLGRGDFGVTSRDRADESWLVAFTADEGGASYYA
                     YDRNNRRGEHLFDARPDLAEYTLATMEPVSFTSRDGLTVEGYLTLPPGSSAEPMPMIL
                     NVHGGPWARDGWGYDPEAQWFANRGYACLQVNFRGSTGYGKKFLNAGNKEWGGTMHDD
                     LVDAVGWAVESGVADPERVAIYGGSYGGYAALAGATFTPDLFRCAVDIVGPSSLITLI
                     NSIPPYWKPLLATFHERVGNPDTEEEFLKSRSPLFFVDRIRIPMLIAQGANDPRVKQA
                     ESEQIVAAMKEKGIDYEYLLFEDEGHGFARPENRLRFYAAAERFLAKHLGGRAEE"
     gene            complement(11079..11867)
                     /gene="dapB"
                     /locus_tag="VN178_02480"
     CDS             complement(11079..11867)
                     /gene="dapB"
                     /locus_tag="VN178_02480"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWS79864.1"
                     /translation="MTRIAVIGAAGRMGREICRAALDTEGIELEGGTVRVGSPELGSD
                     LGGLCGSGKTGVTATVDPPESAEVLIEFTTPGATVEHLSYQRPHVIGTTGLCAEQLAR
                     VEEASHGVPIVLAPNMSLGINLLREVVRGISAKLGEGYDIEVVEAHHRGKKDAPSGTA
                     LFLARAAAEGRGKNLDEVAVYGREGLAPRQEGEIGIHALRGGAVVGEHRLIFYSGGEE
                     VEVIHRALSRRTFADGALRAARFAANAQPGLYSMADVLDTERNV"
     gene            complement(11864..12757)
                     /gene="dapA"
                     /locus_tag="VN178_02485"
     CDS             complement(11864..12757)
                     /gene="dapA"
                     /locus_tag="VN178_02485"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWS79865.1"
                     /translation="MFSGAFTALVTPFRNGEVDVEALEGMVEFQIHNGIHGLVPCGTT
                     GETPAMSEEEDRIVVSTVVRVANGRVPVIAGTGSNSTGMAIKYTKMAEEEGADGSLQV
                     APYYNKPTQEGLYRHFATVAESTELPLILYNIPGRTAVTISAETIARLAELPNIVGVK
                     ESTLSMNMVSDIKSLCGDEFAILSGDDPMTLPMMSLGGQGVISVASNVAPAAVSDMVN
                     ALSMGDWERGRELHYELLPLFRALFVETNPIPVKTAASILGLCSDEMRLPMIPLSGEN
                     LDALRRVMEESNHLLPTPEEV"
     gene            complement(12750..13979)
                     /locus_tag="VN178_02490"
     CDS             complement(12750..13979)
                     /locus_tag="VN178_02490"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWS79866.1"
                     /translation="MGIIVQKYGGSSVATTEHINAVAERIKKTQDSGHGLVVTVSAMG
                     KQTDRLLRLAGEVSRDPSPREIDQLLSTGELQSVALLAMALHDRGVAATSLTGPQAGM
                     KITGRYGSGVISGIQPDRLRGLLDEGQVAIVAGFQGVNALGDIMTLGRGGSDTTAVAL
                     AAALGAERCEVFTDVDGVYTADPRIVPGARRIPVILSSEMAEMSWRGAKVMHPRAVEL
                     GALHGVEIHVKSSFLEGPGTIITGGDGLEHLETRETVAGIVHDLDVSRITLTGIKTGP
                     GTMSRVFSPLAEAGISVDVIVESAPKRGASDVAFTVRRGDFSEALRLAGAVAESLDGH
                     IEGEEELGKVSVVGTGMLNRPGYAARMFATLGEASIPIRLVSTSEIQVTCVLPAGRVE
                     EAVRVLHRDFELEERDV"
     gene            complement(14081..>14956)
                     /locus_tag="VN178_02495"
     CDS             complement(14081..>14956)
                     /locus_tag="VN178_02495"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002827666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWS79867.1"
                     /translation="QDPTQQYPQPEYPEQTQMDQHPGLEREMQPEPDYGVETYKGSGK
                     LEGKKAVITGGDSGIGRAVALAFAREGADVVISYLEEEEADAEEAARAVEDSGRKAVR
                     VPGDVSREARCQELIQRAVDEFGRIDILVNNAAHQMTVEGIADVSTELLDRTFKTNIY
                     AMFWLVKAALPHMPEGGSIINVASIQAYQPSPALLPYSSTKGAIITFTKGLAQEIVQY
                     GLRANTVAPGPVWTPIIPASMPGDTVSSFGGTSPMGRPAQPIELAPAFVFLASQEASY
                     VNGETLGVTGGQPLP"
BASE COUNT         2626 a         4713 c         4876 g         2741 t
ORIGIN      
        1 caccgagaag gagcagacgc tcaacgtcgc aaagagactg gataccctac tcgaaggcga
       61 cggctacacg gtgtgcatga cgcgcacctc caactcgcag accctctcga acaacgcccg
      121 ctacacctac gccaacacca cgggtgcgga tgtcctcgtc tccatccaca tgaacggctc
      181 cacgaatggc tccaccgatt acacgacgac cctcttcggg aagtggcgca aggacaagga
      241 actggccaac accatcttcg gctcgctctc gaccctgcct gcggccagcg gcaccggaac
      301 cataggcacg aggacgccgt actcgttcgc ctcgggcgtc ctcctcaaga gcaacatgcc
      361 agcgaccata gccgagaccg tctttatcac gagcgatagg gaaggacagc tcctctccaa
      421 cggtacggga gcacgccagc agcagatagc gcaagccctg agggtgggca tcgaggaata
      481 cctggccacc cactagcgca cggaagaggc ggaagtcctc gcccgccgtg ctggagcgtt
      541 gggacaaagg gaaccgatga gcctggcgat agagaccgag aagcttacca aggcctacgg
      601 ccgcagggcg cggggcatcg aggatgtgga tctcgccgtc gaggaagggg aggtcttcgg
      661 tttcctgggg cccaacgggg ccggcaagac cacgacggta aggaccctgc tcgggttctt
      721 gcggccgacg ggtgggcgtg gtgaggtctt cggcctggat atcacgaaag acagcgtaga
      781 gataagggcc agggtcggca atctgcccgg agagttcgcc ctggaggaca agatgacggg
      841 agaggggctc ctcaagtact tcgccaggtt gcgtggcgtc ggagatctcg gctacgcgca
      901 cgagatctca gagaggctcg gggccgagtt gcaccgtcct atgcggaggc tatcgcgggg
      961 aaacaagcag aagataggtc tcgtgcaggc catgttccac aggccgcctt tgttgatgct
     1021 cgacgagcct accggcgggc tcgacccgct cgtgcaggag gagtttctcg acatcatcga
     1081 ggaggtcaag gccgagggac gcaccgtctt cttctcctcg cacaaccttg ccgaggtcga
     1141 gcgggcgtgc gaccgggtgg ggatcatccg cggcggtcgg ctcgctgcgg tggagacgac
     1201 ggagacgctc atagacaagt ccttcaggca cgtagtcctc acgttcgcag aggacgtaga
     1261 cagagagccg tttgcgaatc tctcgggcgt caggaacttg aggggcgacg ggaagaggat
     1321 ctccttcacc ctctacgacg accccgacga gatggtcaag ctggcggcca aacacactct
     1381 cgtaaacatg gagtacgaga gaccgagcct ggaggaggtc ttcctcacgt actacgagca
     1441 gaagaacgga gaggtgcgat gagcgtggtt ctggatcgag gccagaagcg aaggcgcgcc
     1501 gcgaggcccg gacggacgct gttcgcgctc gtcacgcaca cggtcaggat gcagttgcgg
     1561 ggtgtgatca tctggggcgc ggccatcgga ctctacggcg ccgcgatggt cgcctcgttc
     1621 acggccatcg aagggagcgc ggtccagctg aaccagctga tggaggccta cccgaagggc
     1681 atgctcgagg ctttcggcgt aaccgacctg ggcgaccctg ccaactacct ccattctcag
     1741 gtcttcgggc tcgcgccgct ggcgctgtct ttcttcccga tactcgcgct cgcgaacgcc
     1801 ctggcagggg ccgaagagcg cggcaccata gacgtccttc tcggcaaccc cctgccgcgc
     1861 tggcagctcg tcgcgggcaa cttcctcgcc ggggccgtct cgctactcgg gatctgcgcc
     1921 atcgtcggcc tgctgacgtg gggcacggcc gtgctcgtcg atgtcgaact ctcccgccga
     1981 gaggtggccg aggccgtcct caacctgtgg cccatctgca tcgtgttcgg cgccctcgcg
     2041 ctcttatgct cggcatcctt ccaccgcaga agtctagcca tagccatccc cgccttcctg
     2101 ctcttcggca tgtaccttat tgacaccata ggaagggtct ccgaagacct cgaagactgg
     2161 aggcctttct ccgtcttcta ctactacggt tcagccatcg agaacggcat agactggata
     2221 cactttggca gcataaccct cgtcgcactc gcactcctgc tcctggccac gctggtcttc
     2281 agacgacgag acatatatac gtagctgtca gcccgccgcc ctgcaggcgg ctcgtcagca
     2341 atcagccgtc agcaagagca ttctggcctt gccttggcgc aatgcgccca tcctcggtag
     2401 acgagggaca cctctagatc atagcgccac acgtaccacg attgccaacc aggctgaccg
     2461 ctgaaagctg acaggccgac tcgttgacat gctagagtct gtacgtcatg gaacgtgcgg
     2521 agacgaaggc actcttgatc agggctcggg ggatcgcgac gtttcgtggt gttctggact
     2581 cggccgttgc gcgggattta cttggtctac tcgagctgct ggaggagtcg cctgacgctg
     2641 gcgctgttgc gaggatcttc ggacggctgt gggaggggct tgccctcgag gaggagcgtc
     2701 tgctccctga cgcctggcag tcacatctcg tgggcagggt tctcgacgat gagaacccgt
     2761 tcagcctcgg ggcggagaag ggggaggccg attcgtccgt gctcgggcag gcacggcggg
     2821 atctgcgcac gctcggggag atgttcgcga tggacgcggc cgagttgctc ggtagggtcg
     2881 agtcggtcgt tcccgccctg gcaggtatct gggtcccctg gaggagtccc gagcccgtgg
     2941 aggaatctcc gcggcgcacg atcgcgcgta aactctcggc ggcggaggac tggggagcgt
     3001 gcgctgagct tcttgccggc cacttcgcca ggcatggagc cggaccctac ggccgccaca
     3061 gggcattcct ctggagagaa gggcggctcc tggccgtctc ccgccccgac cctgtgagcc
     3121 tctccgacct catcacctac gagcgcgagc gggagccttt gatcaggaac accgagcgtt
     3181 ttctcgccgg gctgccggcc caccacgcgc tcctgtacgg cctgccgggg acgggcaagt
     3241 cctccaccgt caaggctatc atgaacgagt acgcggacct ggggctgcgg ctggtcgagg
     3301 tgaagaagga ggacctggcg gagctgccgg gggtactcga agtcctcggc gggcgtgggc
     3361 ggcgtttcgt tctcttcgtc gacgatctct ccttcgagga gcacgaggtc gagtacaagg
     3421 cactcaaggc gctcctcgaa ggcagcgtcg aagagccgcc cgagaacgtc aggctctacg
     3481 cgacctccaa ccgcaggaac ctgatccggg agaccttctc cgaacgcgac gacggtacca
     3541 ctggcgacga cgttcacgcc cgcgacacca tgcaggagaa gctctccctc gcggcccgct
     3601 tcggactacg cctgaccttc ccctctcccg accaggcacg ctacctgaag atcgtatccg
     3661 gactcgccag agaacgcggt ctggccatcc ctgcagaaga cctgcgggag cgcgccctcc
     3721 tgtgggaccg ctggcacgcc ggacgttcgg gccgcaccgc ccgacagttc gtggacgagc
     3781 tcgaagccga ggaacagagc tagtcagcac gctgccttcg gcagcttgtc agcacgctca
     3841 ggcttgcgcc ttcgcttgtc agcacttcag ccagtcgtcc cggaccgttg ccacgagcgt
     3901 cgttctcggc tgccgctggt agcggttcgc catgcgcggc agggcaaggt ccagaacggc
     3961 agaagctgaa atgctgacga gcgagggccc taaaggcccg aagcgtgctg actagctgaa
     4021 aagctaatcg ggcggcgcct cgttgagtac caaccagtag agcctgagtt gctcgacggc
     4081 gttgatgaac tgctcgaaat ccaccggctt gcggacgtag ctgttggcgc cgaggccgta
     4141 gccgtcgagc atgtcccgct gctcattgga cgaggtcagg ataaccacgg gcaagcgcct
     4201 cgtacgttcg tcggaccgca ggcgtttgag tacctcgagc ccgtccaccc tgggcagctt
     4261 gagatcgagc agtatcaact gcggcttgac ggcgggatcc ctccccgcat aggtgccgct
     4321 tgcgaacagg taatcgaggg cctcgacccc atcgtgggcc accacgacct cgttgctgac
     4381 gccggacttc ttgagcgccc gcagggtgag caactcgtcg tcggggttgt cctcgacgag
     4441 gaggatgacc ttgttgctca tctcgtccct ggcctggcgc tggagggcct gcgcgtcttt
     4501 gttatctctg gcactctggt gtatcggcac ggcatctcgt tctgccttcg ggccttgcca
     4561 ggcggctggc gcctcagaca acgccccgcg ggccttctat cctgtccgcg atgtaagggt
     4621 aacggtactc tacacccttg ctgacctggt aggccgcata gcagccgtgt acgaggggca
     4681 ccagcgccag aatgggcagc aagaagaaca taaagatgcc gataatgacg aggctcaaca
     4741 cgaggctgac caaggtgtaa accaccagga tcaagatcca ggctatctgg taccagagcg
     4801 cctgcagggc gtggaagcgc accttctggg agcggtcctt gtatatcagc cagacgagca
     4861 gggccccgaa aggcatgagg ccgacgagcc ccaggagcac gctaaggtgg gcgattatgg
     4921 accacgttcg ctcatcctgc gcggtcatcg tatccatcga ggctccgacc tgctctccgg
     4981 agcctcgttc ccgctcactg ccgaagttgg ggtcttgttg catcttctct ccttcccact
     5041 ctcggagtat acctgtatcc cggccccgcc atgatagcct tgcctgggat gccggcgcat
     5101 cccccaaatg agttattttc ggacgtcttg cccctggcgt cgagttgccc cgacgccctg
     5161 gtaacaatag aatagagttc gatgtgatag aagttacaca gattatcggg gcggggcaag
     5221 agctatcgga cctttgtctg ccatacgcgc atagaaacat gtgccgtggc ccgcgcagag
     5281 atagctggga tcgtgcctga atgggtattc cgctcagcgt cttgctcgtc gaggactccg
     5341 agaacgacgc cctgctcctg ctgaggatgc tgcgcagggg tggatacgac cccacctggg
     5401 agcgggtcga gaccgcggcg gccatggagg ccgcgctcga cggacgcagt tgggatctcg
     5461 tcatctccga ccactccttg ccgaccttca gctcgttggc ggcgctcggg ttgctcaggc
     5521 gcaaggggtt tgtggatctg cctttcataa tagtgagcgg ccggatcggg gaggacgcgg
     5581 ccgtctcggc catgaaggcc ggtgttcacg actacctcat gaaagacaat ctggccaggc
     5641 taaacagcgc catcgagcgg gagctcaggg acgccaaagt acgccgcgag cgccggcaag
     5701 ccgaggagaa gtaccgttcg atcttcgaga acgccgtcga agggatcttc cagacgacgg
     5761 tcgacggacg gtttctgacg gcgaatcccg cgatggccag gatgttgggc tacgagtctc
     5821 ccgaggagct gctcggggcc atctccaata tcagggacca gctctacgta gaaccgggac
     5881 gcagggagga gttctatcag ctcgcgctgc gagatggttt cgtgagtggc ttcgaggtac
     5941 agatgcaccg caaggacgga gaccttctgt ggatctcggc caacgcgcgg gccatctatg
     6001 acaaccatgg agaaatctcg ggttatgagg gtacagtaga gaacattacc gagcgcaaga
     6061 gggccgagga ggccctgcgc gagatccggg aggccgagcg ccgcaggata gcccgtgaac
     6121 tgcacgacgt ggtactgcag gacctgacgt acgccctgca gtccatgcag gtcctccgga
     6181 gattgcccga gggggtagac cgggccgaag agatgggacg ccaggtggag gcgctcaaga
     6241 gggcggtcgg agggctacgc gacgccatct acgatttgcg gctggagagc gccagggagc
     6301 ggcccctggt ccgcgccgta gagtcgatcg tcgaactgaa ccgccagatc ggcgagggct
     6361 gccagttcga gatgatcgtg gaagacgggt ttccggccgc gatctcggga cccgcaggcg
     6421 tcgaggtggt tcgtgtcgtc caggaggcgt tgacgaacgt caggcgccat tcgggcgccg
     6481 gtcgcgcggt ggtcaccctc ggtagggtga acggcgagat cctcgtggag atcgaggacg
     6541 acggcagggg tttcgggccc gagacgtcgt acgggatggg gctcacgggg atgcgcgagc
     6601 gtgtccttgc tctcggcgga gagctcgagg tcgagggtag ggagggcgtg ggaactcgtg
     6661 tgtttctcag ggtaaggatg ggtgtactgg cgggcgggga gaccggagaa cgctcgaaca
     6721 cctgagtcgg cagcggctaa gccgccaaag ctggtatatt gttttcgtgc gtggggtcac
     6781 aagagaggtc tgcgaagagg agagatgcgg cgaattttga tcgtggagga ccacgcttca
     6841 ttccgtcaga cgctggcgtt cgtcttcgat caggagaccg agttcgaggt agtcgcccag
     6901 gcagggactc tcgccgaggc acgccggacg atggatgact ctgaggccga cctcgggatc
     6961 atagacctca gcctacccga cggggagggc accgatctga tcgaagaact gcgcgaagcg
     7021 aacccggact tcgcggcgct cgtcctgacc gcgagtatgg ataggatgga gtacgccagg
     7081 gcggtcgagg ccggggcggc cggcgtcctg cacaagtcgg ccgacgtcga ctcgatactc
     7141 gatgccacgc gacgtgtcgg cgaaggcgag gccctgctct ctgatgacga gctgatctcg
     7201 ttgctccgcc tggccgggca gaaccgcgag gaagaggtag aggcgcgtgc gagcatcgag
     7261 cagatcacac cccgcgaaat ggaggtcctg cagatgctag cctcgggcct ctcgaacaag
     7321 gagatagcgg ccgaactgca catgagcgtg gataccgagc gcacccacat gatgaacatc
     7381 ttgaacaagc tcggcgtgca ctcccggctg caggccctcg tcttcgccgc ccgccacggc
     7441 ctcgtaaaga tacgctgagc gggttccagg aacccgagct gtcagcgatc agctttcagc
     7501 agtcagcttt cgttctagtc ggaagcctgc gcggcaatat gaaccgacca tccttgcaga
     7561 ctacgctagc tccgcagtac caactctcct tcgcagacgt actcggcggg gccttctatg
     7621 tatacgggcc tgccgtggcc ggaccattct atctgtaccg cgccgccgtc catgtacacg
     7681 ctgacggggc tccgggcgag attcttactg actgctgcga ccgctgcggc gcaggaacct
     7741 gaacccgagg cgagggtctc gccggcgccg cgctcccaga tcctcatcct gacgtcgtgc
     7801 ccgccccgcg cgaacccgaa ctctacgttc gtcttctcgg ggaagatggg gtctacttcg
     7861 atgggcggcc cgaccgtagc gaggtccagc gcgtcgaccg cggcctcgct ctccaggaag
     7921 gctaccgcat gtgggttccc catcgagacg cgcaggaagt ggaagccgtc ggactcgacc
     7981 tcggtgtcga cgtggggcgc tcccatgtcg accctggaag agccgtcgtc gaagagcagg
     8041 actttcttga ccccggcccc ggtggcgacg gtcatggcat cgccccccac cataccgcgg
     8101 tccttcgcgt agcggaccag gcagcgcagg ccgttgccgc acatctctga ggtcgagccg
     8161 tcggagttca ggtagatcat ctcgagatcg gcggtctctg agggagcggg aatcaggatg
     8221 ccgtcggccc cgacgccgaa gtgtctgtcg caagccatga gggcgagatc cgcaaggttt
     8281 cgcccctcga cctggccagg gtcgaagatg aggaaatcgt taccgcagcc ctccattttc
     8341 gtgaatcgca tagcggggat tctaccggaa gactcgagca cggttgcggc gagaaaatac
     8401 cgttggaaac ggaatgttcg tcccttatat ttgccctgtg agaagcaacc gcgcgaaata
     8461 tcgtcgtcgc aggatcgggg ccctgttcgt cggggcagca ctcgccgtgg ccgtgtacgc
     8521 tggtggcggg gcaggggccg agccgcggcc cgtccactac accgttgctc cgggagatac
     8581 gctctggggg atagcgctgg agcacacccc aatctgggag gatccgaggc cgaaggtgga
     8641 ggccatccgc gaggccaacg atctctcagg gtatgagatc taccccggca tgagcctgaa
     8701 ggtgccctcg acagtctagg gccttacagc gggttgcaga aggatggacc ctcctgcaac
     8761 ccgcgccgtc agcccacagc tataagcctt cagctttttg ttgttgctga aagctcctcg
     8821 cgcataaggg ttgagccctt atgcacggcg ctgtatacgt cattcccgcg caagcaggaa
     8881 tccacgagaa ggagagcagt gggtcaatgg ctgcgtctgt ggtaccctcg aaacgacgct
     8941 tcggaattgc ctgcaagtcg aggagagatt ttggaagacc gcggcgaggt ttgtctggca
     9001 gaggaggtcg atcgttttcg gcggctgggc ctctctgatg gggagatagc gagcgagatg
     9061 ggcgtcgatt tgagctgggt cgagaccgtg gtctcgtcgg gggaggacga gtcaagtccg
     9121 gacgcggaag accgtgccgt ggatgaaacc tgatgccatc ggccacggtc cgcgcgtagg
     9181 gaagggtttc aaacccgccc ctacaaacga accgcttcat tcttccgcgc gtcctccgag
     9241 gtgtttggcc aggaagcgtt ccgcggccgc gtagaacctc agacggttct cgggacgggc
     9301 gaagccgtgg ccttcgtcct cgaagaggag gtattcgtag tcgatgcctt tctctttcat
     9361 ggcggcgact atctgctccg actcggcctg tttcacgcgg gggtcgttgg cgccctgggc
     9421 tatgagcatg ggtatcctga tgcggtccac gaagaagagg ggggaacggc tcttcaggaa
     9481 ctcctcttcg gtgtcgggat tgccgacccg ctcgtggaag gtcgccagga gcggcttcca
     9541 gtaaggcggg atgctgttta tcagggtgat caggctcgaa ggacccacta tatcgacggc
     9601 gcaccggaag aggtcgggcg tgaacgtggc cccggcgagc gccgcgtacc cgccgtagga
     9661 gcccccgtag atggcgaccc gttcggggtc ggccacgcca ctctcgacgg cccacccgac
     9721 cgcgtcgacc aggtcgtcgt gcatcgtgcc gccccactct ttgttccccg cgttcaagaa
     9781 ctttttgccg taccccgtcg acccccggaa gttcacctgt aggcacgcgt accccctgtt
     9841 ggcgaaccac tgcgcctcgg ggtcgtagcc ccacccgtct cgcgcccacg ggccaccgtg
     9901 gacgttcagg atcatgggca tgggttcggc tgagctccct ggcgggagcg tcaggtagcc
     9961 ctctacggtg aggccgtcgc gggaggtgaa agagacgggc tccattgtgg cgagggtgta
    10021 ctccgcgagg tcgggcctag cgtcgaacag gtgctcgccg cgcctattgt tgcggtcgta
    10081 ggcgtagtaa gaggcgccgc cttcgtcggc ggtgaaggcc acgagccagc tctcgtccgc
    10141 cctgtcgcgg ctcgttacgc cgaagtcacc gcggccgagg ccgtctatga cctcgaagtc
    10201 ctccctgata tcgtcgtcga gcacgatcca ctccgtcctc gcgcgctcca cggcggcggc
    10261 ctgggcctcg tgggtctccg gatgggtcac gacccgcccg atatcgtagc gcgggtcctc
    10321 gacgagcgtc tgagtcgaat cgtcttcgag gtcgagcagg acgagccggg aagcgttggc
    10381 cccacgcgag tctatgaggt acatcttatc gccatcctcg gtaaagccta cgggcgcgct
    10441 cgagagcgcg tcctccttgc cccagccgag tagcttgcgc cagcccgcat cttcgccggt
    10501 tccctcgcgc aggagcaact cgaagccgcc gtccggcgtg gccgccatag cgccgcgcac
    10561 ctcgagttgc gggtctacga cccagcctac ggcgttgcca gggttcctgg ctacgagttc
    10621 gaggtcgccc gtcgagaggg tgaggcggta gacgtcgtgg agctccgggt tctccctgtt
    10681 cagcgcgacg agcagcgtgt cggggaaacg gcggttgctg tccacgatct gggcctgcac
    10741 ttcgtcgaag ggggtcaggt cgcggtcctg tttcgtggac gcatccacgg cgtgcacgcg
    10801 ccagttctcg tcgccgccct cgtcctggag gtagacgatg tgggtgttgt cctcggccca
    10861 gaaatacacc ctgatgccgc gctttctgtc gtcggtcacc ggttcgaagt cctcgcctcc
    10921 ggcggagctc cccaccggac cgacccagac gttcagtacg ccgtctttcg gggccaggta
    10981 ggccaggcgt tctccgtcgg gggagaggtg cggcgccatc ttctccgggt taccgaagag
    11041 gacttcgcgt gggatcaggg gcacggtcga agcgtcggtc atacattcct ctctgtgtcg
    11101 agcacgtcgg ccatagagta caggcccggc tgcgcgttcg cggcgaagcg cgcggccctc
    11161 aaggcgccgt cggcgaaggt gcggcgggag agggcgcgat ggatgacctc gacctcttct
    11221 ccgcccgagt agaagatgag cctgtgctcc cctactaccg cgccgccgcg cagggcgtgg
    11281 atgccgatct caccttcctg cctgggcgcg agaccttccc tcccgtagac cgcgacctcg
    11341 tccaggttct tccctcttcc ttcggctgcg gcgcgggcga ggaacagggc cgtgcccgag
    11401 ggggcatcct tcttgcctct gtggtgggct tctacgacct cgatgtcgta gccctcgccg
    11461 agcttcgccg agatcccgcg caccacctcg cgcaacaggt ttatcccgag gctcatgttc
    11521 ggggcgagta cgatggggac gccgtgcgaa gcctcctcga ccctcgcgag ctgctcggca
    11581 cagaggcccg tcgtcccgat gacgtgaggt ctctggtaag agaggtgctc tacggtcgcc
    11641 cccggggtgg tgaactcgat caggacctcg gcggattcgg gtgggtcgac tgtggcggtt
    11701 accccggtct tcccggagcc gcagagcccg ccgaggtcgg agccgagttc gggggagccc
    11761 acgcggaccg tgccgccttc cagttcgatc ccttccgtat cgagggccgc gcggcagatc
    11821 tcgcgtccca tgcggcccgc ggccccgatt acggctattc tggtcaaact tcttccgggg
    11881 tcgggaggag gtggttcgac tcctccatca cccgacggag ggcgtccagg ttctcgcccg
    11941 acagtgggat catcggcagc cgcatctcat ccgagcacag gccgaggatc gaagccgccg
    12001 tcttgacggg gatgggattc gtctcgacga agagcgcgcg gaagagcggc aagagctcgt
    12061 agtggagctc gcggcccctc tcccaatcgc ccatcgagag ggcgtttacc atgtcggaga
    12121 cggccgccgg ggcgacgttg gaggcgacgg agatcacacc ctgtccccct agagacatca
    12181 taggtagcgt catcgggtca tcgcctgaga ggatggcgaa ctcatccccg cagaggctct
    12241 ttatgtcgct caccatgttc atggagagcg tcgactcctt gaccccgacg atgttgggga
    12301 gctccgcgag gcgggctatg gtctcggcgg agatggtcac ggccgtccgg cctgggatgt
    12361 tgtagaggat gaggggcagt tccgtgctct ccgctaccgt ggcgaagtgc ctgtacagcc
    12421 cctcctgggt gggcttgttg tagtaggggg cgacctgcag ggagccgtcg gcgccctcct
    12481 cctcggccat cttcgtgtat ttgatcgcca tgcccgtgct gttcgagccc gtacccgcga
    12541 taacgggcac ccgcccgttc gcgaccctca ccacggtgct tacgacgatg cggtcctcct
    12601 cttcgctcat agccggggtc tcgcccgtgg tgccacaggg aacgagcccg tggatgccgt
    12661 tatggatctg gaactcgacc attccttcta aggcttcgac gtccacctca ccgttcctga
    12721 aaggtgtgac gagggccgtg aatgcgccgc taaacatctc tctcctccaa ctcgaagtcc
    12781 ctgtgcaaaa ctcttaccgc ttcttctacc cttcccgccg gcaatacgca agtcacctgt
    12841 atctctgagg tagagacgag ccttatgggg atgcttgcct ccccgagcgt cgcgaacatc
    12901 ctcgcggcgt agcccgggcg attgagcatc cccgtcccga cgacggagac cttgccgagc
    12961 tcctcctcgc cctcgatatg cccgtccaga gattcagcga ccgcgcccgc gagccgcagc
    13021 gcttcggaaa agtcgccgcg acggacggtg aaggccacgt ccgaagcgcc tctcttgggt
    13081 gcgctctcca cgatgacgtc gacggagatg cccgcttcgg ccagcgggga gaagacgcgg
    13141 ctcatcgtgc cagggcccgt ctttatgccc gtcagggtga tgcgcgagac gtccaggtcg
    13201 tggactatgc cggcgaccgt ctcgcgggtc tccaggtgtt ccaaaccatc acctcccgtg
    13261 attatcgtac caggaccttc tagaaaggag ctctttacgt ggatctccac cccgtgcaag
    13321 gcccctagct ccacggcccg cgggtgcatc accttcgcgc ccctccagga catctcggcc
    13381 atctccgagg agagtatgac gggtatgcgc cgcgctccgg ggacgatgcg cgggtcggcg
    13441 gtgtagacgc cgtccacgtc cgtgaagacc tcgcagcgct cagcccctag cgccgccgcg
    13501 agcgccacgg ccgtcgtatc cgacccgccg cgaccgagcg tcatgatgtc ccccagggcg
    13561 ttgaccccct ggaagccagc gacgatcgct acctgcccct cgtcgagcaa gccccgcagg
    13621 cggtctggct gtatccccga gatgaccccc gagccgtacc gacccgtgat cttcatcccc
    13681 gcctgcgggc ctgtcaggga ggtggcggcg acacccctgt cgtgcagggc catcgccagg
    13741 agcgcgacgg actgcagctc gcccgtcgag agtagctggt cgatctcccg cggcgaaggg
    13801 tcccgactga cctcgccggc caggcgcagc agcctgtcgg tctgcttgcc catcgccgag
    13861 acggtcacca cgaggccgtg gcccgaatct tgggtcttct ttatccgctc ggcgacagcg
    13921 tttatgtgct ccgtcgtcgc cacggagctc ccgccatact tctggacgat gattcccaag
    13981 gcatgctacc ttccgacgct actgccccag aatagcattt tgtccgatgg ctactgcagg
    14041 agcgtaaagc tgacggctga gagctgaaag ccgaccgctc tcaaggcaat ggctggcctc
    14101 ccgttacgcc gagcgtctcc ccgttgacgt agctcgcttc ctgggaggcg aggaagacga
    14161 aagcaggggc gagctctatc ggctgggccg ggcggcccat gggggaggtt cccccgaagc
    14221 tggagacggt gtcgcccggc atggaggccg ggatgatcgg ggtccacaca ggacccggcg
    14281 ccaccgtgtt cgcccttagc ccgtactgga cgatctcctg ggcgagcccc ttggtgaagg
    14341 tgatgatggc gcctttggtg ctcgagtagg gaagcagtgc cggtgaaggc tggtaggcct
    14401 ggatggaggc cacgttgata atcgacccgc cttccggcat gtgcgggagc gcggccttca
    14461 cgagccagaa catcgcgtag atgttggtct tgaaggtgcg gtcgagcaat tctgtcgaga
    14521 cgtcggctat cccttcgacc gtcatctggt gggccgcgtt gttgacgagt atgtctatcc
    14581 ggccgaactc gtctaccgcc cgctggatga gctcctggca ccgggcctca cgggacacgt
    14641 ctcccggcac cctgacggcc ttgcggcccg agtcttcgac ggcccgtgcc gcctcttcgg
    14701 catcggcctc ctcctcttcc agataagaga ttacgacgtc ggcgccctcg cgggcgaagg
    14761 cgagcgcgac ggcccgtcct atacccgagt cgccgcccgt tatgaccgct ttcttgcctt
    14821 cgagcttgcc gcttcccttg tacgtctcga caccgtaatc tggctcgggc tgcatctccc
    14881 gctccaggcc gggatgctgg tccatctggg tctgctccgg gtactccggc tgcggatact
    14941 gctgcgtcgg gtcctg
//
LOCUS       DATMVF010000055         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_5249, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000055 DATMVF010000000
VERSION     DATMVF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5249"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VN178_02500"
     CDS             complement(<1..1750)
                     /locus_tag="VN178_02500"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79868.1"
                     /translation="MPLTSDSLVSTKLRPSHTRPKLVARPRLAERLDPESGRKLTLVS
                     APAGFGKTTLLSKWFESRVDGDRSVAWLSLDEGDNDPVRFLSYLVGALRSIEEGIGEG
                     VLAALRSPEPPRLEALTGALINEMAALPGRLDLILDDYHLIDSESVHRIVTFLLEHLP
                     EGVHLVISSRVDPPLPLSRLRSRNQIAELGTAELAFTLVEAAAFLKGVMGLELSADDV
                     ARLGGRTEGWIAGLQLAALSMRDRKDISAFIEAFSGSHRDVLDFLAEEVLDRQPPHVQ
                     EFLLATSILDDLTGPLCDALTDRSDGQEMLERLERENLFVVALDDERRWYRYHHLFAE
                     FLRGHLGRESPERMDTLHRSASVWNEQNGGISSAIAHALSAGDHERAARLMERGIGQT
                     WYRGEVATLLGWLGKLPEKTMRRRPLLLIWFAAAMMLAGRFDGVDSLLEEADRVLGNS
                     PGVETTPESEEDAPQHLLATAAAVRSMYSRLQGDPNGAIEHARRALDLLPEDNLNPRP
                     FAAIALAQAYQAAGDPEAAITAFAKAGTLGRAAGHDYVALSAMAAHARLELAQGHLRE
                     ADDVLQRALGYATEHGS"
     gene            complement(1844..2146)
                     /locus_tag="VN178_02505"
     CDS             complement(1844..2146)
                     /locus_tag="VN178_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79869.1"
                     /translation="MSSVIFDISMSLDGFVKASNATPEQPPGDGGERLHEWAFGANDC
                     DREVLAGGVEDSGAVISGRRTHDDSVRWRGADGPRPPTRVFVITHGAPCPAGWASP"
     gene            complement(2220..2714)
                     /locus_tag="VN178_02510"
     CDS             complement(2220..2714)
                     /locus_tag="VN178_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79870.1"
                     /translation="MRKGVLVVHIVSAGAWIGIDVVMGVLIFTALLSADDNTRALCYR
                     ALELFAVWPLLTTGLVCLASGVVLGLGTKWGLVRYWWVAIKLALNILLTALVLVALRP
                     GVMEAAEQGRRFASGEPASLAVGDLIFPPVVSPTALLIAFVLAVFKPWGRIRKRPAPR
                     EDER"
     gene            complement(2980..3510)
                     /locus_tag="VN178_02515"
     CDS             complement(2980..3510)
                     /locus_tag="VN178_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79871.1"
                     /translation="MEMNMDTNGIEPNEPATSTATSKAVEVVGTASPRKAGLARRYLA
                     QGWRFARHLLEMVVAMMFGMGVLSVALAVLGEPPGYANLLLRYGLMGAFMATPMVAWM
                     RYRGHSWSDGGEMTAAMLVPMFALVVPVELGVAVPGLSEGSLMLLSHVAMIAGMVAWM
                     IYRRDRYAHGEHGHHP"
     gene            complement(3959..>5210)
                     /locus_tag="VN178_02520"
     CDS             complement(3959..>5210)
                     /locus_tag="VN178_02520"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS79872.1"
                     /translation="ARRALSLLPAGEAPVRNYATVRLGDALRAVGDVEAAGEAYAEAA
                     EIARSAGHIYGRLAGMVMHARVRAEQGRLREADEAFRRTLRLLTEEGFELSPAAGVVH
                     IGMADLRYERNDLDGAERELERGVELAERTGDVSTLVWAYVTLSRTRRARGDEGSALE
                     MAHKAERVARDSGADLQIAIAAAWLVRLLVARGDLAEAGALGLERSANVGGAAAAARM
                     VDQVTSARLLHAQGSPREALRLLERPHEAAQANGRTRDLIEILSLRALAQWSSNEKER
                     AVGTLAEALVLATPEDYVRSFVDEGPHMVTLMSEVLAVGQKGRTDSLRRVPAHYPRKL
                     MSVLEQNAAGATPSAAGLSEPLSERELEVLRLIASGKSNRQISSELFVSVGTVKTHIN
                     NLYRKIDAHSRTQALARARELQLL"
BASE COUNT          868 a         1783 c         1648 g          911 t
ORIGIN      
        1 cactcccgtg ctcggtcgca tacccgaggg cgcgctgcag aacgtcgtcc gcttcgcgga
       61 ggtgaccctg cgccagttcc agacgggcgt gggcggccat cgcgctaagc gcgacgtagt
      121 cgtggccggc ggcccggccc agagtccccg ccttggcgaa ggccgtgatc gccgcctctg
      181 ggtcgccggc ggcctggtat gcctgcgcca gagcaatggc agcgaacgga cgggggttca
      241 ggttgtcctc gggcaggaga tctagcgccc gcctggcgtg ctcgatggcg ccgtttggat
      301 ctccctgtag gcgagaatac atggaccgca cggcagcagc ggtcgcgagt agatgctggg
      361 gagcatcctc ctcagattcc ggtgtagtct ctacgccagg gctgttgccg agcacacggt
      421 ctgcctcttc gagaagagag tcgacgccgt cgaaccgccc ggccagcatc attgccgctg
      481 cgaaccagat caggagcagt ggccggcggc gcatcgtctt ttcgggcagc ttccctagcc
      541 agccgagcag cgtcgccacc tcgccgcgat accaggtctg gccgattccc cgctccatca
      601 gccgcgccgc ccgttcgtgg tcgccggcgg agagcgcgtg cgcgatggcg gaggagatcc
      661 ctccgttctg ctcattccag acggaggcac tgcggtgcaa tgtgtccatg cgctcgggac
      721 tttcgcgtcc gagatgcccg cggaggaact cggcgaagag gtgatggtag cggtaccacc
      781 tgcgctcgtc gtctaaagca acgacgaaga ggttttcccg ctcgagacgc tccagcatct
      841 cctgcccgtc ggagcggtcc gtgagagcgt cgcacaaagg gcctgtgaga tcgtcgagga
      901 tggaggtcgc aagcagaaat tcctgcacgt gagggggctg cctgtccagc acctcctcgg
      961 ccaggaagtc cagcacgtca cgatggcttc ccgagaaggc ttcgatgaag gcggagatgt
     1021 ccttgcggtc gcgcatggag agggcggcca gttgcaatcc ggcgatccaa ccttccgtcc
     1081 gaccgcccag cctcgcaaca tcatccgccg acagctccag ccccattacc cccttcaaga
     1141 aagcagcggc ctccaccagc gtaaatgcca gctcggcggt cccgagctcg gctatctggt
     1201 tgcgggagcg cagcctcgag agcggcaggg gtgggtctac gcggctcgag atgacgagat
     1261 gcacaccctc cggcagatgc tccagtagga aagtgacgat tcgatgcacg ctttcggagt
     1321 cgatgagatg gtaatcgtcg aggatgagat ccagccggcc aggtagggcg gccatctcgt
     1381 tgatgagggc tcccgtcagc gcctcgagcc taggcggctc gggcgagcgc agcgcggcca
     1441 ggacaccctc cccaatcccc tcctcgatac tccgaagcgc cccaacgagg taggacagga
     1501 atcggacagg gtcgttgtcg ccctcgtcca gagagagcca ggctacggaa cgatccccat
     1561 caactcggct ttcaaaccac ttactcagga gcgtagtctt gccgaagccc gccggggctg
     1621 agacgagcgt cagcttgcga cctgactcag gatccagcct ctcagccagg cgcggcctcg
     1681 ccaccagctt gggacgcgtg tgcgaagggc gtagcttggt cgatacgagc gagtcggacg
     1741 taaggggcac ctacttgctc acctccggtg cctattctac ttccgatcgg tctcggccga
     1801 gggttccgcg gcccccggca tctgtccagc aaaccctgtt cccttacggc gaggcccacc
     1861 ctgccgggca aggcgccccg tgggtgatga cgaacacccg ggttggtggg cgcgggccgt
     1921 ccgcgccccg ccaccgaacc gagtcgtcgt gggtacggcg cccggagatg accgctcccg
     1981 agtcttccac cccgccggcg aggacctcgc ggtcgcagtc gttcgcgccg aacgcccact
     2041 cgtggagtcg ttcgcctccg tcgcccggtg gctgctcggg ggtagcgttc gaggccttca
     2101 cgaacccgtc cagggacatg cttatgtcga agatgacgct gctcatcggg gcctcccggc
     2161 ctccctgttg gtgaacagga ttttgttccg cggccagcgt ccccaggcga ccatgccgct
     2221 caacgctcgt cttcccgtgg cgccgggcgc ttacggattc ggccccacgg cttgaacacc
     2281 gccagcacga acgcgatgag cagggcggtc ggagagacca ccggcgggaa gatcaggtcg
     2341 cccacagcga gcgaggccgg ttcaccgctc gcgaaccgcc ggccctgctc ggccgcctcc
     2401 atcacccctg gccgcaacgc gaccagaacc agcgcggtga gcaggatgtt cagggccagc
     2461 ttgatcgcca cccaccagta gcgcaccagt ccccacttcg tgccgagccc cagcacgacg
     2521 ccgctggcga ggcacacgag gccggtcgtc aaaagcggcc agaccgcgaa caactcgagc
     2581 gccctgtagc agagcgccct ggtgttgtcg tcagcggaga gcagggcggt gaagatgagg
     2641 acgcccatca ctacgtcgat gccgatccag gctccggccg acacgatgtg cacgaccagg
     2701 acgcccttgc gcatccgagc acccaatcgc cacggccgag gacggtctgc tgggctaagg
     2761 ctcgtcatta cgctgtgctc actcagcctt ggcttggcct gagagctttt cgccgaacgc
     2821 cgtagttcga atctcggggt gcgccatggg cgaccgttct gcgtagctct cagtcatgac
     2881 gactgctcct tcgcgttgct ttgtttagcc tcgggggcaa gctggccgtt ctccggcatc
     2941 cggctctgcc gaacctcgga cgacgttttg gtggacgagc tacgggtggt gaccgtgctc
     3001 cccatgcgcg tagcggtccc ggcggtagat catccaggcg accatcccgg cgatcatcgc
     3061 cacgtgcgag agcagcatca gcgacccctc ggagagccct ggcaccgcca cgcccaactc
     3121 caccggcacc accagggcga acatcggcac gagcatcgct gccgtcatct ccccgccgtc
     3181 ggaccaggag tggccccggt aacgcatcca cgccaccatc ggcgtggcca taaacgcgcc
     3241 catcaggccg tacctgagta gcaaattggc gtagccaggc ggctcgccca gcaccgcgag
     3301 cgccacgctg aggaccccca tcccgaacat catcgcgacg accatctcca ggaggtggcg
     3361 cgcgaagcgc cagccctggg cgaggtagcg acgcgccagc cccgccttcc tcggcgatgc
     3421 ggtgcccacc acctccaccg ctttgctcgt agccgtggag gttgccggct cgttgggttc
     3481 gatgccgttg gtgtccatgt tcatctccat tctctcgtcc ccttgctcaa acgctttcgt
     3541 ctctgcaggc cccatgccgt cggtggcgtg ctcgcggacc tcatccgcga agcccttttc
     3601 gtcggtaatg atctttgctc caatcgtcgg ttggctctcg gcaagaaatc taccgtccga
     3661 ccggatggga gacatccacc aaatggtaga tttcgggggt ggagatactc ctatccggct
     3721 cgtcgtagcc tcgcccgccg tactggcgtc cggcagtacg accaaggaga ttccaaaggt
     3781 gtgagcacgt cgcgtcccga atggcgggga ctgaaagcgc atgagcagtt cgggactgtg
     3841 cttcgtcgtc cggctcgatc cggggtggcg gccctcggga gctgctaatc tcttcggcaa
     3901 caaccattcg actatgagtg ctttacggtg gaacgtgtaa agctccaggg aggggttact
     3961 agagcagttg cagctctcta gcccgcgcca gggcttgagt gcggctgtga gcgtcgattt
     4021 ttcggtagag attgttgatg tgggtcttga cggtgcccac gcttacgaat agttcggagg
     4081 atatctggcg attactcttg cctgaggcta ttagccggag gacttccagc tcccgttcac
     4141 tcaagggttc ggatagtccg gcagccgatg gtgtagcgcc agcggcgttc tgctccagca
     4201 cggacataag cttcctgggg tagtgtgccg gcacgcggcg tagtgagtcc gttcgtcctt
     4261 tctgccctac tgcgagcacc tccgacatga gcgtgaccat atgaggaccc tcgtccacga
     4321 aggacctgac gtagtcctcc ggtgtagcca acaccagggc ttccgcgagc gtgcccaccg
     4381 cccgctcttt ctcgttggac gaccactgcg ccaaagcccg tagcgacagg atctctatga
     4441 gatcgcgcgt acgcccgttt gcttgtgcgg cttcgtgcgg tctctccagc agccgcagcg
     4501 cctcgcgggg actaccctgc gcgtggagta gcctcgccga ggtgacctgg tccaccatcc
     4561 gcgcggcggc tgcagcgccg cccacgttag cggaccgctc tagtccgaga gcgccggctt
     4621 cggcgaggtc tcctcgcgcc acaagcagcc ggaccagcca agccgccgcg atggcgatct
     4681 gtagatcggc gccagagtcg cgggcgacgc gctccgcttt gtgggccatc tcgagggcgc
     4741 tcccctcgtc cccgcgcgcc cgcctcgtcc gcgagagggt gacgtacgcc caaaccaggg
     4801 tgctgacgtc cccggtacgt tcggcaagtt ccacgcccct ctccagctcg cgctccgccc
     4861 cgtccaggtc gttgcgttcg tagcggaggt ccgccatccc tatgtggacc acccccgcgg
     4921 cgggcgacag ctcgaagcct tcttcggtca gcagccgcag cgtacgccga aacgcttcgt
     4981 ccgcttcccg caggcgcccc tgctcagccc gcaccctggc gtgcatcacc atacctgcga
     5041 gcctaccgta gatgtgaccc gcggaccggg caatctcggc ggcctccgcg taggcctcgc
     5101 ccgccgcctc cacatcgccg acggccctga gcgcgtcacc caggcggacg gtagcgtagt
     5161 tgcgaacggg agcctctccg gcgggtagga gcgagagggc ccgccgggca
//
LOCUS       DATMVF010000056         6567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_468347, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000056 DATMVF010000000
VERSION     DATMVF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468347"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VN178_02525"
     CDS             complement(<1..864)
                     /locus_tag="VN178_02525"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79873.1"
                     /translation="MEERERSGWTWRLAGLLAPVTIWLGLFFLVPLLLILIFSFGRSG
                     VYGGITLGFNPGNYLKVFDPLYLEIVGRTFVIALTNTLLCLALGYPLAYFIVFKGHRW
                     RNALILLVMVPFWTSLLVRAYSWVVILNGNGIANRTLQFLGITDEPVTLIFTPQAVTM
                     GMVYSYLPFMVLPLYAALEKLDVSLKEAAQDLGASRWHTFWRVTFPLSMPGVIAGSIL
                     VFIPSAGEFVIPNLLGGSRTVLMGNLIQQQFLQARDWAFGSALSVMLAVLLLGAIMFY
                     IRRVGTDKLEGV"
     gene            complement(867..1976)
                     /locus_tag="VN178_02530"
     CDS             complement(867..1976)
                     /locus_tag="VN178_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWS79874.1"
                     /translation="MGREKRFSRGDFLKVVGAGVVASSTLSTLSGCTVSTTAESGGGG
                     GTGNENALNLYNWSDYVNPKTTIPNFEKKYGVEVTQDFYSDNEALLAKLQAGGTGYDV
                     IVPSDYMVSVMRKSDIIQKLDRSKIPNFDNLSADFRGLPFDPNNDYSMPYQWGTTGIL
                     YNKKELGEISSWDVLWDTEYKGEIAMMSDVRETMGAALKLLGYSLNSTKPEQLEEAKQ
                     LLLEQKPLLRGYFASFEVRPLVQNGDVLLGHVYSGDGFLAIAENKDLDYAIPEEGATR
                     WVDNMAIPVGAEHVDNAYKFVNYILGAKAGASLTNYTFYGSPNEAAEPMIKKEILDDP
                     RIYPPPEVFERLEVIEDIGEATRKYERIFTEVKSA"
     gene            complement(1979..3109)
                     /locus_tag="VN178_02535"
     CDS             complement(1979..3109)
                     /locus_tag="VN178_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS79875.1"
                     /translation="MEERRVSERPPQEVEDAGRGVERTVAVELSAVTKRFGEFVAVGD
                     LTLDIYEGEFFSLLGPSGCGKTTTLRMIAGFEEPTEGGISVDGDQMSGVPPYRRPVNT
                     VFQSYAIFPHLNVFDNVAFGLRRAGVRGDELRKRVTEACEMVQLAGFERRKPNMLSGG
                     QQQRVALARALVNHPKVLLLDEPLGALDLKLRKEMQLYLKNLQHEVGITFIYVTHDQE
                     EALTMSDRIAVMNEGRVQQVADPPTLYERPKNRFVADFIGQTNVFSGTVESVNGERVT
                     LRTASGERIEATAHEVELGEEADAAVRPEKIRFGSEGDNTSSARIRQIVYLGVSTQYI
                     AELPDGQELVLYQQNFREEDHPEEGEEVSVAWDARNCLILGG"
     gene            complement(3169..4593)
                     /locus_tag="VN178_02540"
     CDS             complement(3169..4593)
                     /locus_tag="VN178_02540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS79876.1"
                     /translation="MTSAPSARPEEKDYRSYSFWLETSREELLPRPSLGGSIEADVAI
                     LGAGFTGLWTAYYLLAREPSMKVVVLESEIAGFGASGRNGAWCSSGFPVSPGELQRRF
                     GREAARELLTEMRGAVEEVGRVARAEEIDAQYFRGGLLRIARGPSQLPGIRDAYESLR
                     GLGLEDGIRLLDADETAGRVRITGARGALFNPHCATIHPARLARGLARAVERLGGEIF
                     ERSPVTDYEEGGENGSGPRLVTDSGVVRARTVVLAGEAYLARLRKLRRQLLPIYSLIV
                     LTEPLSEERWAGVGWEGRECVASNRYTVDYLSRTEDGRILFGGRGAPYHYGSRVSDEY
                     DRHEPTHQMLRSTARDWFPALEGARFTHAWGGPLAVPRDWMPTMSYDPSSGVATARGY
                     TGQGVATANLSGRTLADLILERQTDITRLPTVGHEQRPWEPEPLRWLGARYVQRGLMK
                     VDDNAERTGEPPTGKTLAERLGRH"
     gene            complement(4605..5969)
                     /locus_tag="VN178_02545"
     CDS             complement(4605..5969)
                     /locus_tag="VN178_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWS79877.1"
                     /translation="MDVMRGLEESGSSAPHPGVERLMLHFTPYSEDWSKLPVIVSGEG
                     CYVTDDKGKSYIDGLAGLFTTQVGHGRSELAEVAARQMKELGFFPNWSMHHPRSLELA
                     ARISEIAPGDLSSTFFVSSGSEAVETVIKLARQYHKANGEAERYKVISRKIAYHGTTM
                     GALSVTGLPAFKAPFEPLLQGFYHVPNTYEDPEGAADAIEEAIEFGPPETVAAVILEP
                     VQNAGGCLVPPPDYWERVREICDRHGVLLVSDAVICAFGRLGEWFGIERFDVVPDMTS
                     FAKGVTSGYLPMGGVVVNEKMSATLEGNAPMFMHGSTFGGHPVSSAVALENIDIIERE
                     GLLKNVHELEGHFGDELKRMAADHPIVKEVRGMGFFWAVEVNPERADGTPLEGDEYDK
                     YFKGVVTKKLLEGGLICRFDDKEDPVIQFSPALVADKEIISKIAGITDDALTELEREL
                     GYRG"
     gene            complement(6199..>6567)
                     /locus_tag="VN178_02550"
     CDS             complement(6199..>6567)
                     /locus_tag="VN178_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79878.1"
                     /translation="QEVAQELFGKAEEQADAYRRFFGQWGNFPMGSFPFPGMTNSVMF
                     PFQKQGMRLVETAARGAEETIATATFPIAGYDEKNVDEISARLDGLSAEQIRHLRAYE
                     KSNKNRQSLIDRFDSKLRAS"
BASE COUNT         1137 a         2192 c         1927 g         1311 t
ORIGIN      
        1 caccccctcc agcttgtccg tgcccaccct gcggatgtag aacatgatgg ccccgaggag
       61 taatacggcg agcatcaccg agagcgcgct cccgaatgcc cagtcacgtg cctggagaaa
      121 ctgctgctgg atcaggttcc ccatcagcac cgtccgcgac ccgccgagca ggttcgggat
      181 aacgaactct cccgccgatg gtatgaacac aaggatgctg cccgcgatca cgccgggcat
      241 gcttaagggg aacgtaaccc gccagaacgt gtgccagcgg ctggccccca ggtcctgcgc
      301 cgcctccttc aggctgacgt cgagcttctc cagggccgcg tacagcggca ggaccataaa
      361 cggcaggtag gagtagacca tccccatcgt taccgcctgg ggcgtaaaga tcagggtgac
      421 gggctcgtcg gtgatcccga ggaactgcag cgtgcggttg gcgatgccgt tgccgttcaa
      481 gataaccacc caggagtagg cccgtaccaa taggctcgtc caaaacggga ccattacgag
      541 caggatcagg gcgttgcgcc acctgtggcc cttgaagacg ataaagtacg ccagcgggta
      601 gcccagggcc agacacagca gcgtgttcgt cagcgcgatc acgaaggtgc ggcccacgat
      661 ctccagatac agggggtcga agaccttgag gtagttgccc ggattgaagc ccagtgttat
      721 gccgccgtag accccgcttc tcccgaagga gaagatcagg atcagcaaca gcggcaccaa
      781 aaagaagagc ccgagccaga tcgtgacggg ggccaaaaga cccgctaacc tccaggtcca
      841 cccgctcctt tcgcgctctt ccattctcag gcgctcttta cttcggtgaa gatgcgttcg
      901 tacttgcggg tcgcctcccc gatgtcctcg atcacctcca accgctcgaa gacttccggc
      961 ggcgggtaga tgcgcggatc gtcgaggatc tccttcttta tcatgggttc ggcggcttcg
     1021 ttgggcgagc cgtagaacgt gtagtttgtt agcgacgcgc ccgccttcgc gcccaagatg
     1081 tagtttacga acttgtaggc gttgtctacg tgctctgcac ccaccggtat ggccatgttg
     1141 tccacccagc gggtggcccc ctcttcgggg atggcatagt ccaggtcctt gttctcggcg
     1201 atggccagaa agccatcccc gctgtatacg tgccccaaca ggacgtcacc gttctggacc
     1261 aggggcctga cctcgaacga ggcgaagtac cctcgcaaga ggggcttctg ctccaggagc
     1321 aactgcttgg cctcttccaa ctgctccggc ttggtgctgt tcaaggagta tcccagcagc
     1381 ttgagagccg cccccatggt ctccctgacg tcgctcatca tcgcgatctc gcccttgtac
     1441 tcggtgtccc acaatacgtc ccagctgcta atctctccca gctctttctt gttgtagagg
     1501 atgcccgtgg ttccccactg gtagggcatg ctgtagtcgt tgttcggatc gaatggaagt
     1561 cccctaaaat ccgcgctgag gttgtcgaag ttcgggatct tggacctgtc cagcttctgg
     1621 ataatgtcgc tcttgcgcat gaccgaaacc atgtagtccg aggggacgat gacgtcgtat
     1681 cccgtacctc cggcctggag cttcgccagc aacgcctcgt tgtcggagta gaagtcctgg
     1741 gttacttcta ccccgtactt cttctcgaag ttggggatcg tagtcttggg gtttacgtag
     1801 tccgaccagt tgtagaggtt gagcgcgttc tcgttgccag tgcctccacc tccaccgctc
     1861 tcggcggtcg tgctgacggt gcagcccgaa agggtcgaga gcgtcgagct ggccaccacc
     1921 cccgcgccga ccaccttaag gaaatcgccc ctggagaacc tcttctctct acccatgatc
     1981 atcctcccag tatcaaacag ttacgggcgt cccacgccac cgacacttct tcgccttctt
     2041 cgggatggtc ctcctcgcga aaattctgct ggtagagcac caactcctgc ccgtcgggga
     2101 gttccgctat gtactgggtg ctgaccccga ggtagacgat ctgccttatc ctggcggagc
     2161 tcgtgttgtc gccctcgcta ccgaaccgga tcttctcggg cctcacggcc gcgtccgcct
     2221 cctccccgag ctcgacctca tgagcggtgg cttcgatcct ctcccccgaa gcggtacgca
     2281 acgttacgcg ttcaccgttc acggactcga ccgtacccga gaatacgttc gtctggccga
     2341 taaagtccgc cacaaaacgg ttcttcgggc gctcgtagag ggtcgggggg tcggctacct
     2401 gctgcaccct gccctcgttc atcacggcga tcctgtcgct catggtgagg gcctcttcct
     2461 ggtcgtgggt gacgtagatg aaggtgatcc caacctcgtg ctgcagattc ttgaggtaga
     2521 gctgcatctc tttcctgagc ttgaggtcta gcgctcccag cggctcgtcc aacagcaaca
     2581 ccttgggatg gttgacgagc gccctcgcga gcgcgaccct ttgctgctgc ccgcccgaga
     2641 gcatgttcgg cttgcgcctc tcgaagcctg cgagctgcac catctcgcag gcctccgtga
     2701 ccctcttgcg caactcatct cccctgacgc cggcgcggcg caggccgaag gccacgttgt
     2761 cgaagacgtt taggtggggg aagatagcgt aggactggaa gacggtgttt accggcctcc
     2821 tgtagggagg taccccgctc atctggtccc cgtcgacgga gataccgccc tcggtcggtt
     2881 cttcgaagcc ggcgatcatg cgcagcgtcg tggtcttgcc gcacccgcta gggccaagca
     2941 gcgagaagaa ctctccctcg tagatgtcga gcgtgaggtc gccgaccgcg acgaactctc
     3001 caaagcgctt ggtaaccgcg gacaactcca cggcgacggt acgttccaca ccccggcctg
     3061 cgtcttccac ctcttgcggc gggcgctcgc ttacacgtcg ctcttccacc ctcagcctcc
     3121 tgtagcgtcc atagtggccc ttccccgctt atgattctag ctgtagattc agtgtctgcc
     3181 gagccgttct gccagggtct tgcccgtcgg cggctctccc gttcgttcgg cgttatcgtc
     3241 caccttcatc aggccgcgct gcacgtacct ggcgcccagc caacgcaagg gttccggctc
     3301 ccagggacgc tgctcgtggc cgacggtggg caatcgggtt atgtcggtct ggcgttcgag
     3361 gatcaggtcc gccagggtcc ggcccgagag gttggccgtc gcgaccccct gccccgtgta
     3421 gccacgcgcg gtggctacac cactggacgg gtcgtaagac atggtcggca tccagtcgcg
     3481 gggtacggcc agcggccctc cccacgcgtg ggtgaaccgc gcgccttcta gggcagggaa
     3541 ccagtcccta gccgtgcttc gcagcatctg gtgcgtcggc tcgtgcctgt cgtactcgtc
     3601 gcttacgcgc gagccgtaat ggtacggtgc gcccctaccc ccgaacaata tgcgcccgtc
     3661 ttcggtacgc gagaggtagt cgaccgtgta gcggttggac gccacgcact cgcgcccttc
     3721 ccagccgacg ccggcccacc gctcctcgga gaggggctcg gtgagcacga tgagggagta
     3781 gatggggagt aactggcggc gcaacttgcg gagccgcgcc aggtaggcct cgccggcgag
     3841 cacgaccgtg cgcgcccgaa ccactcccga gtccgtaacc agcctcgggc ccgagccgtt
     3901 ctcgccaccc tcctcatagt ccgtcacggg cgaccgctcg aagatctcac cccccagccg
     3961 ttcgacggcc ctggcgagcc cgcgcgcgag ccgcgcgggg tggatggtcg cgcaatgggg
     4021 attgaacagc gcgcctctgg cgccggtgat cctgacgcgc cctgccgtct catccgcgtc
     4081 gagcagcctg atcccgtcct ccaggccaag cccgcgcagc gactcgtacg catcccttat
     4141 acctggtagc tggctcgggc cacgcgctat gcggagcagt ccgccccgaa agtactgggc
     4201 gtcgatctcc tccgccctgg ctaccctgcc tacttcctcg accgcgcccc gcatctcggt
     4261 caagagctcc cttgcggcct ctctcccgaa gcgccgctgg agctccccgg ggctgacggg
     4321 gaagcccgaa gagcaccagg ccccgttgcg cccggaggcc ccgaaaccgg cgatctccga
     4381 ctcgagcacc accaccttca tcgagggctc tcgggcgagc agatagtagg ccgtccacag
     4441 gcccgtaaag ccggcgccga ggatggccac gtcggcctcg atagatccgc ctagcgaagg
     4501 tctggggagg agttcctccc tagacgtctc caaccagaag ctgtaggagc ggtaatcctt
     4561 ctcttcgggg cgggcgctag gggcgctggt caaactctac ccctctagcc ccggtagccg
     4621 agttctcgtt cgagctccgt gagggcatcg tcggttatgc ctgctatctt ggagatgatc
     4681 tccttgtctg ctacgagcgc cggggagaac tggatgacgg gatcttcttt gtcgtcgaag
     4741 cggcaaatga gaccgccctc caacagcttc tttgtgacga cacccttgaa gtacttgtcg
     4801 tactcgtcac cctccagcgg cgtaccgtcg gcccgttcgg gattgacctc cacggcccag
     4861 aagaagccca tccccctgac ttccttgacg atgggatggt cggcggccat cctcttcagc
     4921 tcgtcgccga agtgcccttc gagttcgtgg acattcttta gcagcccctc gcgctcgatg
     4981 atatctatgt tctcgagggc gaccgcgctc gaaacggggt gccccccgaa ggtcgagccg
     5041 tgcatgaaca tcggggcgtt gccctcgagc gtcgcgctca tcttctcgtt gaccacgacc
     5101 ccgcccatcg gcaggtagcc cgacgttacg cccttggcga agctcgtcat gtccgggacc
     5161 acgtcgaagc gctcgatgcc gaaccactcg cccagccgtc cgaaggcaca gatcaccgcg
     5221 tccgagacga ggagtactcc gtgcctgtca cagatctccc gcacccgctc ccagtaatcc
     5281 ggtggcggca cgaggcaccc acccgcgttc tgcaccggct ccaggattac ggcagccacc
     5341 gtctccggcg gtccgaactc tatggcctcc tcgattgcat ccgcagcccc ctcagggtcc
     5401 tcgtaggtgt tcggtacatg atagaaaccc tgcaagagcg gctcgaaggg agccttgaac
     5461 gcgggcaatc ccgtcacgga gagcgcgccc atcgtggtgc cgtggtaggc gatcttgcgc
     5521 gatatcacct tgtagcgctc ggcctcgccg ttcgccttgt ggtactggcg ggccagcttg
     5581 atgaccgtct ctacagcctc cgaacccgag gagacgaaga aggtggagga gaggtcccca
     5641 ggcgctatct cggagatcct ggccgcaagc tccagcgacc tgggatggtg catgctccag
     5701 ttggggaaga agccgagctc cttcatctgc ctcgcggcga cctcggcaag ctcgctcctc
     5761 ccgtgcccga cctgggtcgt gaagagcccg gcgagcccgt ctatgtaaga tttgcccttg
     5821 tcgtcggtga cgtagcaccc ctcaccagag acgattacag gcagcttgga ccagtcctcc
     5881 gagtaaggcg tgaagtggag catcagtctc tccacaccag gatgcggcgc gctgctcccg
     5941 ctctcttcga gacccctcat cacgtccatg taatacctcc tacaggcatg gtcacccggc
     6001 tcctcacttt gcacgaatac tacacagtta ctgtaatggg gtcaagatcc aacgcggatg
     6061 acgagatccg tgttcgatcc ctgtggcgct attgtaatcg gtgtatcaca tgaaatgcga
     6121 actagcacct tgggagaaag cacgaagaga ggccggggct agaaccccgg cctcttcgtt
     6181 acgctcgtgt agcttttgct acgaagccct gagcttgctg tcgaaccggt cgatcaggct
     6241 ctggcggttc ttgttggact tctcgtaagc cctgaggtgc cggatctgct cggcgctcag
     6301 accatcgagc cgcgcggaga tctcatcgac gttcttctcg tcgtagccgg cgatggggaa
     6361 ggtggccgtc gctatggtct cctccgcgcc ccttgcggcc gtctctacga gtcgcatccc
     6421 ctgcttctgg aaggggaaca ttacgctgtt ggtcatgccg gggaacggga agcttcccat
     6481 ggggaagttg ccccactgcc cgaagaaacg cctgtaggcg tccgcctgct cctcagcctt
     6541 cccgaagagt tcctgcgcca cctcctg
//
LOCUS       DATMVF010000057         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_284077, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000057 DATMVF010000000
VERSION     DATMVF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284077"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VN178_02555"
     CDS             <1..299
                     /locus_tag="VN178_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HWS79879.1"
                     /translation="LAQTLQDTGYGEEGEIIGFDLYPYTEDQVAAVRRAIVQWEFIWD
                     LARKIDRAELREAKSRADALAGQKAVYAALGMDEGYEAEIAVRRQEARASRNET"
     gene            296..1795
                     /gene="xylB"
                     /locus_tag="VN178_02560"
     CDS             296..1795
                     /gene="xylB"
                     /locus_tag="VN178_02560"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HWS79880.1"
                     /translation="MSVLIGLDVGTGGARAVAVDESGEIVAEASSEYPLYSPHPGWTE
                     QDPVDWWAGTKEVVKNVAVQTGEQVTGIGLTGQMHGSVFLDSSDEVIRPALLWNDQRT
                     GRQCEVITTAVGEERLISISGNPALTGFQAPKIVWLKEEEPENFGRVSRVLLPKDYVR
                     LRLTGEYATDASDAAGTLLLDMRARDWSGEILNSLEIPQDWMPEVYEGPENTGVLRES
                     VAEELGLPRGIPVAAGGGDNAAAAVGTGIVGTGLVNSSVGTSGVLFAHASEFNPDPSG
                     RLHAFCHAVPGAYHLMGVTLSAGGSLSWWRETLGGDYDELVEAASDVRPGSEGLVFLP
                     YLSGERTPHLDPKARGAFFGLTARHGVEHMTRAAMEGVIFSLRDSLDIMRGLGIPVED
                     VRATGGGARSELWRQLQADIYGTPIRRTVADEGPAYGAALLAGVASGTYTSVDEAVSV
                     VKLREEITEPDSDRAKIYEEYYEVYRSLYPATSSAMSHLTDLATGSNGE"
     gene            1847..2716
                     /locus_tag="VN178_02565"
     CDS             1847..2716
                     /locus_tag="VN178_02565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS79881.1"
                     /translation="MSASERCTDEAVLRESARWVHVPPDGVRLEDERRLLVHLPKRWG
                     QSRVWRSSAVDEEKAEELIEDTIGAVRAAGGGRLVWHTGEGISPPFMDECLAKRGFET
                     NEELDVLAFSLVDGLEPSLPRLGVPEDVSAELVRDADGLREALLVDSEVFSSPPPSGD
                     EFAGYARELAKLGLRERGESVEEGASLALRFLAFVDVGFEYVREDEQKALGAAGAQMV
                     GETLRLWGAATREAFRGRGAYRALVLERCRLGRELGATLALTKANTASGTTLKRAGFT
                     VIAKERRHVLEVP"
     gene            2716..3246
                     /locus_tag="VN178_02570"
     CDS             2716..3246
                     /locus_tag="VN178_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWS79882.1"
                     /translation="MSSGMETPGKWGVADRKRLILIKHSEPSIDPEVPPNSWRLSEEG
                     RRKSMTLAARLRPYGPDIVLTSEEPKATETARIVAGRLGLELIMHPRLHEHDRTGAPF
                     GTQDDFERSTKVYFENPGELVWGNETAEQAGERFSGAMDEILERYQDINVAVVAHGIV
                     ITLFVARYASVDLFAF"
     gene            complement(3475..4215)
                     /locus_tag="VN178_02575"
     CDS             complement(3475..4215)
                     /locus_tag="VN178_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS79883.1"
                     /translation="MVEKGRKPVSREAYEKLADAYSARAPTKPHNAYYERPATLSLLG
                     DVGGKRVLDAGCGPGIYAEELVARGAEVVGFDASGRMVDLARERLQGRAEVSRANLEE
                     PLAWLEDESFDLVVSALAMDYVEDWRGPLTEFHRVLRPAGKLVFSVEHPTFKFIEQVY
                     EGEGSYFETALGGMEWTGFGEMVWMPSYRRPLGAMVDAILDSGFVLGGMLEPRPDERF
                     RIADPEEYERLSRMPGFLCLVGRKPRGA"
     gene            complement(4396..4569)
                     /locus_tag="VN178_02580"
     CDS             complement(4396..4569)
                     /locus_tag="VN178_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79884.1"
                     /translation="MSATRGKPTRVATYVRVSKGDGSHETITPSTTRNMKVRDRDLPC
                     SSRKFKKCRCSNA"
     gene            complement(4637..4909)
                     /locus_tag="VN178_02585"
     CDS             complement(4637..4909)
                     /locus_tag="VN178_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79885.1"
                     /translation="MAGHATISIWFGDPEEAVRKSAVLDNWCRKVGRDPREIERSVPI
                     HPDRLGEAAAYVERGFDHLIVGFHNATDDPSSLKKLVARRDSRNEG"
BASE COUNT          995 a         1783 c         1754 g          917 t
ORIGIN      
        1 agctggccca gaccctccag gacacgggct acggggaaga aggcgagatc atcggcttcg
       61 acctctatcc ctacacggaa gatcaggtgg ccgccgtcag acgcgccatc gtgcagtggg
      121 agttcatctg ggacctggcc aggaagatag acagggccga actgcgcgag gccaagtccc
      181 gcgccgacgc ccttgccggc cagaaggcgg tctacgccgc gctcggcatg gacgaaggtt
      241 atgaggcgga gatcgcggtg cgccgccaag aggcgcgcgc cagcaggaat gagacgtgag
      301 cgtcctgatc gggctcgacg tcggaaccgg cggggcgagg gccgtcgccg tggacgagtc
      361 gggagagata gtcgccgaag cctcatcgga gtacccgctc tacagcccgc accctggctg
      421 gaccgagcag gacccagtcg actggtgggc gggcaccaaa gaggtagtaa agaacgtcgc
      481 cgtgcagaca ggggagcagg taacaggtat cgggctcacc gggcagatgc acggctccgt
      541 gttcctcgat tcgtcagatg aggtgataag gccggcgctc ctctggaacg accagcgcac
      601 cggcaggcag tgcgaggtga ttaccactgc cgtgggggag gagcgtctga tctccatctc
      661 aggcaacccg gccctcacag gatttcaggc accgaagatc gtctggttga aagaagaaga
      721 gcccgaaaat ttcgggcggg tctcccgcgt gctcctgccc aaggactacg tgaggctccg
      781 gctcaccggc gagtacgcca ctgacgcctc ggacgcggct ggcaccctgc tcctcgacat
      841 gagggcccga gactggtcgg gtgagatcct aaactccctg gagatcccac aagactggat
      901 gcccgaagtc tacgaaggcc ccgagaacac gggcgtcctt cgcgagagcg tggccgaaga
      961 gctgggcttg ccgcggggca tccccgtggc ggcgggcggc ggggacaacg cggcggcggc
     1021 cgtcggtacg ggtatcgtcg gcaccgggct cgtcaactct tccgtcggca cgagcggcgt
     1081 actcttcgcc cacgccagcg agttcaaccc cgacccctcg ggacgactgc atgccttctg
     1141 ccacgccgtg ccaggtgctt accatttgat gggcgtcacg ctctccgcgg gagggtccct
     1201 atcctggtgg cgcgagacgc tggggggcga ctacgacgaa ctggtcgagg ctgcctcgga
     1261 cgtcaggcct ggctccgaag ggctcgtgtt cctgccttat ctctcgggcg agcggacgcc
     1321 gcacctcgac cccaaggcga ggggggcttt cttcgggctc acggcccgtc acggagtgga
     1381 gcacatgacc cgcgccgcga tggagggtgt gatcttctcc cttcgcgact ctctggacat
     1441 tatgcgcgga ctcggcatac ccgtggagga cgtcagggct accggcggcg gggcgagaag
     1501 cgagctctgg cgccagctcc aggccgacat ctacggcacc cccatacgca ggaccgtcgc
     1561 cgacgaggga ccggcctacg gcgcagcact cctggctggg gtggcctcag gcacctacac
     1621 cagcgtggac gaagccgtct ccgtggtgaa actacgagag gagatcaccg agcccgatag
     1681 tgaccgggcg aagatctacg aggagtacta cgaggtctac cgctcgctct acccggcgac
     1741 gagttccgcc atgtcccacc tcacggacct cgccaccggc tcgaacggcg agtaaccctc
     1801 aacagatggc gtagccagca gaacgacctt gcgctacgct catcacgtga gtgcaagcga
     1861 acgatgtact gacgaagccg tactccggga atccgcgcgc tgggttcacg tcccacccga
     1921 cggcgtgcgc ctggaggacg agcggcgtct gctggtgcac ctaccaaagc gttggggcca
     1981 aagcagagtt tggcgctctt ctgccgtcga cgaggagaag gcggaggagc tgatcgaaga
     2041 taccatcggc gcggtccgcg ccgccggcgg gggcaggctc gtgtggcaca cgggcgaggg
     2101 cataagcccg ccgttcatgg acgagtgcct ggcaaaacgt ggcttcgaaa ccaacgagga
     2161 gctcgacgtc ctcgccttct ctctcgttga cggtctagag ccgagtctac cccggctcgg
     2221 cgttccagag gacgtaagcg ccgagctcgt gcgcgacgcc gacgggctaa gggaggcgct
     2281 cctcgtagac tcggaggtct tctcctcgcc tccaccttct ggggacgaat tcgccgggta
     2341 cgccagagaa ctagcgaagc ttggcctccg cgagcgcggt gaatcagtcg aagagggtgc
     2401 atcgctcgcc ctgaggttct tagcattcgt ggatgtgggt ttcgagtatg taagagaaga
     2461 tgaacaaaaa gcattgggcg cggcgggcgc gcaaatggtg ggcgagacgc tcaggttgtg
     2521 gggcgctgcg acacgcgagg cgttcagggg caggggcgcc taccgggcgc tcgttctaga
     2581 aaggtgccgg ttagggaggg aactcggggc gaccctcgcc ctgaccaagg cgaacaccgc
     2641 ctccggaacg acactcaaac gcgcaggctt cacggtcatt gccaaagagc ggcgccacgt
     2701 gctagaagtc ccctagtgtc gagcggaatg gagacgcccg gaaagtgggg ggtggccgac
     2761 cgcaagaggc tcattttgat caaacactcc gagccctcga tcgatccgga agtgccgccg
     2821 aactcctgga gactctcgga ggaaggtcga cgcaagagca tgacgctggc cgcgcgtctc
     2881 cgcccatacg gacctgacat cgtcctgacg agtgaggagc ccaaggcgac cgagacggcg
     2941 cggatagtcg ccggccggtt agggctggag ctcatcatgc acccacggtt gcacgagcac
     3001 gaccgcaccg gcgcgccctt tggcacccag gatgacttcg agcggtccac aaaggtctac
     3061 ttcgagaacc caggagagct cgtgtggggc aacgagacgg ccgagcaagc gggggagcgc
     3121 ttctcaggtg cgatggacga gatcctggag cggtaccagg atattaacgt ggctgtggtg
     3181 gcccacggca tcgtgatcac gctcttcgtc gcccggtacg ccagcgtgga cctcttcgcc
     3241 ttttgaaagc ggctcgggct tccttcgttc tgcgtactga catctctgcc tttccggcta
     3301 gaacacgcag tcttccattt ataggtggtg tcgcaatcgt cttcaggagg agcacccgcg
     3361 ccgctcagaa ccccgttcac ccggcttctc aatagggagc atcaagaggc gccgagccga
     3421 caggaggggc ggcctggcgg cgatgccgca ggtagcggcg cggccaggct aggcctacgc
     3481 accccggggt ttgcgtccca ctaagcagag gaacccgggc atccgggaga gcctctcgta
     3541 ctcctccggg tctgcgatcc gaaagcgctc gtcgggcctg ggctcgagca tgccccccaa
     3601 gacgaaaccg ctgtccagga tggcgtcgac catggcgccc aggggcctcc ggtacgacgg
     3661 catccacacc atctccccga accccgtcca ctccatgccc ccgagcgcgg tctcgaagta
     3721 gctgccctct ccctcgtaga cctgctcgat gaacttgaag gtgggatgct ccaccgagaa
     3781 caccagcttc ccagcgggcc tgagcaccct gtggaactcc gtgagcggtc cgcgccaatc
     3841 ctccacgtag tccatggcca gcgcgctcac cacgaggtcg aaggattcat cttccagcca
     3901 cgcgagcggc tcctcgaggt tcgcgcggga gacctccgcc ctgccctgga gcctctcccg
     3961 agccagatcg accatccgcc cgctggcatc gaagccgaca acctcggcgc ccctggccac
     4021 gagctcttcg gcgtagatac ccggtccgca accggcgtcg agcacccgct tgcccccgac
     4081 gtctccgagc agggacaacg tcgcgggacg ctcgtagtag gcgttgtggg gcttggtcgg
     4141 ggcgcgggcc gagtaggcgt cagccagctt ttcgtatgcc tcacgcgata ccggcttgcg
     4201 tcccttctct accataacgc tccttcgtgg gtgctagaca agcagatcaa cgaaaccaca
     4261 cggacggtct cggcgcaata cgcaccgaac ctggcagatg cgccaaaagc gccccataaa
     4321 caaaattctt cacaaatctt aacaacaacg agccgaggat ctaccggtag tttcctgcca
     4381 cgtcgaagtc gatcctcagg cgttgctgca gcggcacttc ttgaacttcc tgctgctgca
     4441 cggaaggtcg cggtctctga ctttcatgtt gcgcgtcgta gacggcgtaa tggtctcgtg
     4501 agaaccgtcg cccttggaga ccctgacgta ggtcgccacc cgcgtcggct tgcctctcgt
     4561 cgcgctcatt ggctttgccc ctagagcgaa ctcgaggtgc cgtcgcgccg gtacccggct
     4621 tctagcccga gccagcctag ccttcgttac gcgagtcgcg ccgggccacc agtttcttca
     4681 gggacgaggg gtcgtcggtc gcgttgtgga agccgacgat gaggtggtcg aatccccgtt
     4741 cgacgtaggc ggcggcctcg cccaagcgat ccgggtgaat ggggacggag cgttctatct
     4801 ccctcgggtc gcgtccgacc ttccggcacc agttatcgag cacggcgctc ttacggaccg
     4861 cctcctccgg atcgccgaac cagatcgaga tcgtcgcgtg tccggccacg atgcgcagcg
     4921 tgaccttctc acctccgccc cgatcatgac cggcatctcg cgcgtgggcg gcgggttcag
     4981 cttgcccagc cgcgccccga tcgtttccac cgaggcgtcg aggtccctga gtctctcacc
     5041 cgccgtgccg aactcctagc cgtactcttt cctgcaggat ctgtgtcccc atcctcacgg
     5101 ccatcccacg ccttctgttc accatggtcg ccgcctcagg acatattatg ccttctcggc
     5161 ctcgcggaaa gagactacgc gcccaggaag ccgcgtcgct cccggatgcc gcagcaagtc
     5221 cttttcgcct acgcggacgc gatcttagac cacgacaggg tgccgggcca taaaccggct
     5281 cagagggtac gggcggctta ttcggccggg ggcgtcgcgc cgagcccggg ctatcatcgt
     5341 tctggcagcc tagtgtcctc cctgtgcgcg cttagggcgc tggtgtagcg acgcagcatc
     5401 tcgggcatca tcactttggc cggcccgcgc tccacgccga agacagtgc
//
LOCUS       DATMVF010000058         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_602682, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000058 DATMVF010000000
VERSION     DATMVF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602682"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VN178_02590"
     CDS             complement(<1..994)
                     /locus_tag="VN178_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79886.1"
                     /translation="MKTESPGVEAATALLRRFEPVIRSTTGDKFYPMDVEPYVRACSL
                     WVQRPGEEAVCVVPGGKLSLDRLAQQPMDEAGAVHFLKFTDTEDLRSDSPGGRLRVFR
                     SRAKDRKESTRAFRAGRGRLARVGYFSRFVDALYSIALLARGRVPGESAAAASVAYER
                     IMAGQEHYRYHGRVLRQDGWIVLQYWLFYPFNDWRSGFFGANDHEADWEKVFVYLSET
                     EAGEVRPEWVAYAAHNYTGDNLRRRWDDPELEKVGEHPVIYVGAGSHASYFAPGEYLT
                     ELTLPLPSPLARVAGAIRTFWKTRLGQYVGDGEEDGGSGYFSIPFVDYARGDGLS"
     gene            complement(984..1376)
                     /locus_tag="VN178_02595"
     CDS             complement(984..1376)
                     /locus_tag="VN178_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79887.1"
                     /translation="MRVIGVLLILEVIGLVLLGGYEFLQVDWRDIDLRTLQPQAVELA
                     VFALFIPSAILTLLSALSFLFLYRRGWLLAAIAQGLSLAVCLWLYSWFQPGYVYPIMA
                     YCVLMILYLNSQDVRMVFRTGQQASHEN"
     gene            complement(1488..>2201)
                     /locus_tag="VN178_02600"
     CDS             complement(1488..>2201)
                     /locus_tag="VN178_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWS79888.1"
                     /translation="RAVFADIESAGRAAVAMIRTGMRIGRVELVDARTIEAVNAYKGT
                     DYAAAPTLFLEFSGSKAGVESDAEVARETSAAEGCSSFEVEVDEEAREKLWEARHDAA
                     LAIRDLYPGMGMMGTDVCVPISDLPGALRHARDTIGSRGLHGAILGHVGDGNYHAVFP
                     VGTGDEADQERAESVNAEIVDYALERGGTCTGEHGIGMGKTGHLQKEHGDSLPFMREI
                     KSIADPNGIMNPGKIFSGG"
BASE COUNT          390 a          757 c          676 g          378 t
ORIGIN      
        1 cgctcaggcc gtctccgcgc gcgtagtcta caaaggggat gctgaagtag cccgacccgc
       61 cgtcttcttc gccatccccc acgtactggc cgagcctcgt cttccagaaa gtgcggatgg
      121 ctccggccac cctcgcgagg ggtgaaggca gggggagggt cagttccgtc aggtactcgc
      181 ccggcgcgaa gtagctggcg tgggagccgg cgccgacgta gatcacgggg tgctccccca
      241 ctttctcgag ctccggatcg tcccagcggc gcctgaggtt gtcccccgtg tagttgtggg
      301 ccgcgtaggc cacccactcc gggcgtacct caccggcctc cgtctccgag aggtacacga
      361 acaccttctc ccagtcggcc tcgtggtcgt tcgcgccgaa gaacccgctc ctccagtcgt
      421 tgaacgggta gaacagccag tactggagta cgatccagcc gtcctggcgc agcacgcgac
      481 cgtggtaacg gtagtgttct tgcccggcca ttatgcgctc gtaggccacc gaagccgccg
      541 cggccgattc cccaggaacc cttccgcggg cgaggagcgc tatcgagtaa agggcgtcga
      601 cgaagcggga gaagtacccg acgcgggcca gccggccccg cccggccctg aaggccctgg
      661 tcgactcctt gcggtccttc gcgcgactgc ggaacacgcg caggcgtccg ccgggcgagt
      721 cggacctcag atcctcggta tccgtgaact tgaggaaatg tacggcgccg gcctcgtcca
      781 tgggctgctg cgcgagccgg tcgaggctca gcttcccacc gggcacgacg catacggcct
      841 cctcaccagg cctctgcacc cagaggctgc aggcccgcac gtagggttcg acgtccatcg
      901 ggtagaactt gtcccctgtg gtcgagcgga tcacgggctc gaaacgccgg agcagggccg
      961 tggctgcctc cacgccgggg ctctcagttt tcatgcgacg cctgctgtcc ggttcggaac
     1021 accatccgca cgtcctgaga gttcaggtag aggatcatca gtacgcagta ggccatgatc
     1081 gggtagacgt agcctggctg gaaccaggag tagagccaca ggcacacggc caggctcaac
     1141 ccctgcgcga tcgcggccag aagccacccc cttcggtaca agaacaggaa gctcagcgcc
     1201 gagagaagcg tcagtatggc cgaagggatg aagagggcga agaccgccaa ctcgacggcc
     1261 tgaggctgca gcgtcctcag gtcgatatcc ctccaatcga cctgtaagaa ctcgtatccg
     1321 ccgagcaaga cgagcccgat cacctcgagg ataaggagca ccccgatcac cctcacaggc
     1381 cggtatgatg tcttctctgg ttctaccagg gtctagcttt ccctcgccta cccgccaatt
     1441 ctacagcccg ccgtgactgt agcccaacga cgaggaaagc aagccgatca tcctccgctg
     1501 aagatcttgc ccgggttcat gatgccgttc gggtcggcga tactcttgat ctcgcgcata
     1561 aacggcaaag agtcgccgtg ctctttctgc aagtgtccgg tctttcccat cccgatacca
     1621 tgctctccgg tacaggtgcc gccgcgctcc agggcgtagt ccacgatctc ggcgttgacc
     1681 gactcggcgc gctcctggtc tgcctcgtcg cccgtgccta ctggaaagac agcgtggtag
     1741 ttcccgtcac cgacgtggcc cagaatggcc ccgtggaggc cacgggaccc gatggtgtcg
     1801 cgggcgtgtc gcaaggcgcc gggaaggtcg gagatcggga cgcacacgtc cgtgcccatc
     1861 attcccatac ctgggtagag atcgcgaatg gcaagggccg cgtcgtgacg ggcctcccag
     1921 agcttctccc gggcctcctc gtccacctcc acctcgaacg aagagcaacc ttccgccgcc
     1981 gaggtttcgc gggccacctc cgcgtcgctc tcgacgcccg ccttcgagcc gctgaactcc
     2041 aggaagaggg tcggggcggc agcataatct gtgcccttgt aggcgtttac agcctcgatc
     2101 gtgcgggcgt cgaccagctc gacgcgaccg atcctcatcc cggtccggat catcgccaca
     2161 gcggcgcgtc ccgccgactc gatgtcagcg aagaccgccc t
//
LOCUS       DATMVF010000059         6261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_648404, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000059 DATMVF010000000
VERSION     DATMVF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648404"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VN178_02605"
     CDS             <1..433
                     /locus_tag="VN178_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79889.1"
                     /translation="ESKAECLESGDFEAFLRDWYAQPLFAPLARDEDLLRRTIDARRR
                     NDAGELARSLRGMGTGSQPSLWGELTGLGVPALAIAGELDGKYAGISSRMAGINPRIE
                     SAVVPGAGHTVHAETPAAYNSLLWRYVEGLSFALGETKPVP"
     gene            complement(395..811)
                     /locus_tag="VN178_02610"
     CDS             complement(395..811)
                     /locus_tag="VN178_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS79890.1"
                     /translation="MIQRQSHSTIYVIDQEEALRFYRDILGFEVNTDMPMGDSGLRWL
                     TLNPKGQPDLELILAQVASGPMFDAETAEEMRAMLERGAFGIGVFETDDIKGDYERLS
                     SQGVEFVSPPQERFYGTEAIVKDNSGNWFSLTQSKR"
     gene            complement(815..1276)
                     /locus_tag="VN178_02615"
     CDS             complement(815..1276)
                     /locus_tag="VN178_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWS79891.1"
                     /translation="MRAARDFMRHAYGRPVSLPDVAARANLSPYHFLRVYKRAYGETP
                     HEFLTRLRIERAKTLLASGSHNVTEACFEVGFSSLGSFSNLFAYRVGLPPSEYRRYAR
                     SMISVPSTVRTLFVPSCFFTMLYGVLEERNFREAGEAPPLVSSEYSNETGR"
     gene            1426..1911
                     /locus_tag="VN178_02620"
     CDS             1426..1911
                     /locus_tag="VN178_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS79892.1"
                     /translation="MNIEFKRLSEVDSADMIKLANDPRVRRHMPLSSGTFGEADYERF
                     LRDKERLWEEHGYGPWAFFVDGKFASWGGLQPEDGDADVALVLNPHYWGTGRAIYEEI
                     ITRAFDEMGFDSVTALLPPSRTNVRGLMRLGFEPDGETEIGSERFIRYRLRAPRYRPA
                     Q"
     gene            1945..2796
                     /gene="menB"
                     /locus_tag="VN178_02625"
     CDS             1945..2796
                     /gene="menB"
                     /locus_tag="VN178_02625"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HWS79893.1"
                     /translation="MTNIQWESAREFEDIRYEKAEGIAKITINRPEVRNAFRPLTVIE
                     MQEALEDAREDHETGVIILTGEGPDAFCSGGDQKVRGDAGYLDDPDAPRRTPGGESVG
                     RFHVTDLHVQIRRCPKPVVAMVAGYAVGGGHVLHVVCDLTIAADNAKFGQVGPKVGSF
                     DGGFGASVLTSLVGPKKAKEMWFLCRIYSAEEALEMGLVNTVVPLAALEEETVRWCRE
                     MLDRSPFALRLLKASFHAHEDGYAGIQQLAHDANLLFYGSEEAQEGRDAYKQKRKPDF
                     SKFPRRP"
     gene            complement(3011..4213)
                     /locus_tag="VN178_02630"
     CDS             complement(3011..4213)
                     /locus_tag="VN178_02630"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HWS79894.1"
                     /translation="MGNVRRRLTDGSNQRASTLELFYDLVFVFAITQVSHLLLEHLTW
                     AGVLQSLIVLLAVYWSWNYTTWTTNELDTETVPVRLLLLALMLVSLLMAVAIPQAFEA
                     HALLFAGSYVAIQIGRHTFLTFAAADRGTIERERAGHILTWFVVVGVLWVAGALLDGP
                     ERVVLWLVALALDYCAPLVTYWVPGRQRLAPATWNVGTEHFAERFGLFIILALGESIV
                     ITGATTSELELDAARIIAFVMAFLASAAIWWLYFTSVSTIGEHYLEVSENRTILARDA
                     YTYLHVVFVAGIILSAVGDELVIAHPTEILPPYEVAAVAAGPAVYLFAHTLFGYRLTG
                     VVYKAKVLGTLACFAVGFLGLLVPALVLAGLLILVLVAVIVSSYLAPSSAEAEGEALL
                     QSSSWKAE"
     gene            4403..4573
                     /locus_tag="VN178_02635"
     CDS             4403..4573
                     /locus_tag="VN178_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79895.1"
                     /translation="MAAGIADSPVPAGLDPYRAIAEDVLETPLDLPSPEVGVRETTDA
                     SRTVDVRRLEWL"
     gene            4679..>6261
                     /locus_tag="VN178_02640"
     CDS             4679..>6261
                     /locus_tag="VN178_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWS79896.1"
                     /translation="MLAQLRNVSLSFPDKKVLEDVSFTVYPEDRISLVGENGAGKTSI
                     FRILKGSLTPDTGEVSLSRGVRIGYLEQDFAGMEEDAKRTCMEVALEPFGSLIELERR
                     IEVLASELGGVGESERTSEILADLGEAQHRFEVSGGYEFRSRTRSTLTGLGLSEAYWG
                     SSVSELSAGQRVRLALARLLLEDHDLILFDEPTNHLDVPAREWLEGHLAGMDTAYVVA
                     SHDRRFLDAVSRKVAHLDRGRLTLYSGDYTAFRQQLRQAEEVGWRRYEKNRKLAKKLQ
                     RQAQDYQRWSDAGEKEKRGAADKGFVSHKAAKVMKRSLVARRRMEEAIENARTEKPFE
                     KDAVKIEFGASKGRSLLRADDLIVGYSRDRPLTTELSLELWAGDRLAILGPNGCGKTA
                     LLRTVLGEIPPLHGHVRLSPSSKAGYFDQDNRLVPVDVTALEAVLGTGRDETLVRTVM
                     GRMGVRRETVNKKVAKLSSGERAKVLLAGLILGDNNLLVLDEPTNYLDIETQDVLLEA
                     LVDFPGGILFVSHDRYFVE"
BASE COUNT         1277 a         1882 c         1969 g         1133 t
ORIGIN      
        1 tgagtcgaag gcagagtgcc tggaatcggg agacttcgag gcgtttcttc gggactggta
       61 cgcgcagccg ctcttcgcgc ccctggcgcg ggacgaggac ctgctccgtc ggactatcga
      121 tgcgcggcgg cgcaacgacg ctggagagct ggcgcgctcg ttgcgaggga tgggtacggg
      181 gagccagcct tcgttgtggg gagagctgac aggtctaggc gtaccggcgc tcgccatagc
      241 cggagagctc gatgggaagt acgccgggat ctcatcgcgc atggcgggca tcaacccgcg
      301 aatagagtcc gcggtcgtcc ctggagcggg acataccgtg cacgccgaga ctccggcggc
      361 atacaactcc ttgctctggc gttatgtgga aggactatcg tttgctctgg gtgagactaa
      421 accagttccc tgagttgtct ttgacgatgg cctctgtgcc gtagaaccgt tcctggggtg
      481 gcgacacgaa ctcgacaccc tggctgctca agcgctcgta gtcgcctttg atgtcatccg
      541 tttcgaagac gccgatgccg aaggcgcctc tctcgagcat cgccctcatc tcttccgccg
      601 tctcggcgtc gaacatcggt ccagaggcta cctgggccag gatcaactcg aggtcgggtt
      661 ggcctttcgg gttcagcgtc agccagcgaa gccccgagtc gcccatgggc atgtcggtgt
      721 ttacctcgaa gcccaggatg tcgcggtaga acctgagggc ttcttcctgg tcgattacgt
      781 agatcgtaga gtgactctgg cgttggatca cgacttacct ccctgtctcg ttgctatatt
      841 ctgaagatac tagcggaggt gcctctccgg cttctcgaaa attgcgctct tccagaactc
      901 cgtacaacat cgtgaaaaag cacgagggaa cgaatagcgt ccttaccgtc gaaggaacgg
      961 agatcatcga ccgagcatag cgcctgtact cggaaggggg taggccaaca cgatacgcga
     1021 acaggttact gaaactgccc aggctggaga agccgacctc gaagcacgct tccgtgacgt
     1081 tgtgactccc actcgccaaa agcgtcttcg cccgctctat gcgcagccgg gtcaggaact
     1141 cgtgcggtgt ctctccgtag gcccgcttgt agacgcgcag gaagtggtaa ggggagaggt
     1201 tggcccgggc cgcgacgtcg ggtagagaga cgggacgccc gtaggcgtgg cgcatgaagt
     1261 cgcgagctgc tctcaaatct ctgtgcgtct gggggtgcat cccgatggaa gggtacagtc
     1321 ggtgctgccg gaatcaacgg cgatctttgc gcgctacctg aggaccgccc tcccgccatc
     1381 ggtctatcat taggcgtgag cgtgcagcga gccagaggtc gaaagatgaa catcgagttc
     1441 aagaggttga gtgaggtaga cagtgccgac atgatcaagc tagcgaacga tcctcgggtg
     1501 cgaagacaca tgccgttgtc gagcggcacc ttcggtgaag ccgactacga gaggttcttg
     1561 agggacaagg agcgactctg ggaagagcac gggtacgggc catgggcgtt cttcgtagac
     1621 ggtaagtttg ccagctgggg tggtcttcag cccgaggacg gggatgcgga tgtggcgctg
     1681 gtcctcaacc cgcactactg gggaacggga cgcgccatct acgaagagat catcacccgc
     1741 gcgttcgacg agatgggctt cgattccgtg acagccttgt tgccaccttc gagaacgaac
     1801 gttagggggt tgatgcggct cggattcgag ccggatgggg agaccgagat cggcagcgag
     1861 cgcttcattc gttaccggct ccgcgcgcca cgctaccgcc cagctcaatg aaagaatcaa
     1921 gcgcaaatct ctataatctg ctctatgact aacatacagt gggaatcggc cagagagttc
     1981 gaggacatca ggtacgagaa ggcggagggg atcgcgaaga tcaccataaa ccgccctgag
     2041 gtgcgcaacg ccttccgtcc cctgacggtc atcgagatgc aagaagccct cgaagacgcc
     2101 cgcgaagatc acgagaccgg ggtcatcatc ctgacggggg agggacctga cgcattctgt
     2161 tccggaggag accagaaggt acgaggggac gccgggtacc tcgatgaccc cgacgcgccc
     2221 cgtcgcactc cggggggaga gtctgtcgga cgcttccacg tcacggatct gcacgtccag
     2281 atccggcgtt gccccaaacc cgtcgtcgcg atggtggcgg gatacgccgt cggcgggggc
     2341 cacgtcctgc acgtggtgtg cgacctcacc atcgctgccg ataacgcgaa gttcgggcag
     2401 gtcggcccaa aggtcggaag cttcgacggc ggattcggcg cgtcggtcct aaccagcctc
     2461 gtcgggccga agaaggccaa ggagatgtgg ttcctctgca ggatctattc cgccgaggag
     2521 gctctagaga tgggactagt caacaccgtc gtgccgctcg ccgcgctcga ggaggagacc
     2581 gtccgctggt gccgcgagat gctggaccgc tcgccattcg ccctgcgcct cctgaaggcg
     2641 agcttccacg cccacgagga cggctacgcc ggcatccaac agctcgccca cgacgcgaac
     2701 ctactcttct acggaagcga agaggcacaa gaaggccgcg acgcctacaa acagaagcgt
     2761 aaacccgact tctcgaagtt cccccgccgt ccctgaccgc gcccagtaat tctctgcaac
     2821 ggtcacttcc tgaaacctgt agcctgatgc ccggattgcg gaactatacc aagtccgtcc
     2881 catcaccatc ggataggcat ccctaagcgt cattcccgcg caagcgggaa tccacgtcct
     2941 ttggagtcct tgtcggatac gaagcgatct cgtggctgcc tgacatgcct ctcttcttgc
     3001 ggacttcgta tcactcagcc ttccacgagc tgctttgaag cagagcttcg ccctccgcct
     3061 cggcactcga aggtgccaga tagctagata cgatcaccgc caccaaaacc aagataagca
     3121 gccccgccaa caccagcgca ggtacgagga ggccgaggaa gccgacggcg aagcaggcca
     3181 gcgtcccgag gaccttcgcc ttgtagacca cccccgtcag tctgtagccg aatagggtgt
     3241 gggcgaagag gtacacggcc ggcccggctg ccaccgccgc aacttcgtac ggcggcagga
     3301 tctccgtcgg atgggcgatc accaactcgt ctcccaccgc ggagaggatg atcccggcga
     3361 cgaacaccac gtgcagatag gtgtaggcgt ctctggcgag aatcgtcctg ttctcggaga
     3421 cctcgaggta atgttcccca atcgtcgaca cggacgtgaa gtacaaccac cagattgccg
     3481 ctgacgccaa aaaagccatc acgaaggcta tgatcctcgc cgcgtcgagt tcgagctctg
     3541 aagttgtcgc gccggttatg acgatagact cgccgagcgc gaggatgatg aacaggccga
     3601 agcgctcggc gaagtgctcc gttccgacgt tccaggtggc tggcgcgagg cgctgccggc
     3661 ccggtaccca gtaagtaacg agcggcgcac agtagtcgag cgcgagcgcg accagccaga
     3721 gtacgacgcg ttccgggccg tcgagcaggg ctcctgccac ccacagtacg ccaaccacca
     3781 caaaccaggt gaggatgtgt cccgcccgct cgcgttctat cgtcccgcga tccgccgccg
     3841 cgaaggtcag gaaggtgtgc ctgccgatct gtatggcaac gtacgacccg gcaaacagca
     3901 gcgcatgagc ctcgaacgcc tgcggtatcg caaccgccat gaggagactg acgagcatta
     3961 gcgccaggag cagcagtcgg acagggacgg tctccgtatc cagttcgttg gtggtccagg
     4021 tcgtgtagtt ccacgaccag tacaccgcga ggaggactat cagggactgc aggacgccgg
     4081 cccacgtcag gtgctcgagg aggaggtggg agacctgcgt gatcgcgaac acgaacacga
     4141 gatcatagaa gagctcgagc gtcgaagccc tctggttcga cccatccgtc agccgtcgcc
     4201 tcacatttcc cacgagagca gtctaaccac gttcgggatg ttctgccgcg gtagcagtcc
     4261 ccgaaggcgc gacgagcgaa gtctgtttgt cgttccgttc aactcctgcg aatacgcgag
     4321 cacgcctcgt ttctgcaccc gttcgctaca agtgtcaata tgacgaccag atgacaccgt
     4381 tacgctgtca ggaatcaccg gaatggcggc cggcatcgca gacagccctg ttcctgccgg
     4441 actggatccc taccgggcga tagccgaaga tgttctcgag acgccgctgg acctgccgtc
     4501 tcctgaagtg ggcgtcaggg agacgacgga tgcttcacgc acggtagacg ttcgcaggct
     4561 ggagtggcta tagcggctct gtgcgaggcg gagggcgctg gagtatactc tcgggcatgt
     4621 agcgagcctc ctttgcaata tgctttcctc gtcctctcgt cctgcaaagg atctttacgt
     4681 gctcgcccaa ctcagaaacg tctcgctctc gttccccgac aagaaggtac tagaagacgt
     4741 ctccttcacc gtctatcctg aggataggat ctccctcgta ggagagaacg gcgcggggaa
     4801 gaccagcatt ttccgcattc tgaaggggtc tctgacaccg gacacgggcg aggtctcgct
     4861 gtcacgggga gtacgtatcg ggtatctgga gcaggacttc gcaggcatgg aggaggatgc
     4921 gaaacgtacc tgcatggagg tagcgctcga gcctttcggg tccctgatcg aactggagag
     4981 acgtatcgag gtcctcgcct ccgagctcgg cggggtggga gagagcgaac gcacctcgga
     5041 gatactggcc gatctgggcg aggcgcagca tcgcttcgag gtctcgggtg ggtacgagtt
     5101 cagatcgagg acacgatcta ctctgacggg tcttggcctc tccgaggcgt actggggcag
     5161 cagtgtctcc gagctctccg ccggccagag ggtgcgcctc gcgctcgcga ggctgttgct
     5221 cgaggatcat gacctcatcc tcttcgacga gccgaccaac cacctcgatg tgcccgcccg
     5281 cgaatggctg gagggacacc tggcagggat ggataccgcc tacgtcgtgg cttcccacga
     5341 ccggcgcttt ctcgatgccg tctccaggaa agtcgcccac ctcgatcggg gcagacttac
     5401 cctctattct ggcgactaca ccgcgttcag gcaacaactt aggcaggccg aggaggtagg
     5461 atggcgcaga tacgagaaaa accggaagct ggcgaagaag ctgcagcggc aggcccagga
     5521 ctaccagaga tggtcagacg caggcgagaa agagaagcgg ggcgcggcgg acaaagggtt
     5581 tgtaagccac aaggctgcga aggtcatgaa gcgttcgctc gtcgcccgac gcaggatgga
     5641 ggaggccatc gagaacgccc gtacggagaa gccgttcgag aaggatgcgg tcaagataga
     5701 gttcggggcc tcgaagggcc gcagcctcct gcgcgccgat gatcttatag tcggttactc
     5761 cagagaccgg cccttgacca cagagctttc tctcgagctc tgggctgggg acaggctggc
     5821 tatcctcggt ccgaatggtt gcggcaagac cgcgcttctg cgtaccgtgc tcggtgagat
     5881 cccacccctg cacggccatg ttcgtctctc accctcctca aaggccggtt acttcgacca
     5941 ggacaaccgc ctcgtgcctg tcgacgttac ggcgctggag gcggtcctcg ggaccgggcg
     6001 cgacgagacc ctggtccgta ccgtcatggg caggatgggc gtcaggaggg agaccgtcaa
     6061 caagaaggtg gcgaagttga gctcaggcga acgcgccaag gtgctcctgg ccgggctcat
     6121 cctcggggac aacaacctcc tcgtcctcga cgagccaacc aattacctcg acatagagac
     6181 ccaggacgta ctgctggagg cgctcgtaga cttccccggc gggatcctgt tcgtctccca
     6241 cgaccgttac ttcgtggaag a
//
LOCUS       DATMVF010000060         5605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_510486, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000060 DATMVF010000000
VERSION     DATMVF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510486"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VN178_02645"
     CDS             complement(<1..649)
                     /locus_tag="VN178_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HWS79897.1"
                     /translation="MKVDELHGFDLSPSEARRLQEELAPRVVTGPALDLSGVRHVAGA
                     DVSTQGDTAYATVVVLEFPGLSLVEVQGFEAPLRFPYVPGLLSFREVPSLAGALREVE
                     TGVEALILDAQGLAHPRRMGLASHLGIFLDVPTVGCAKSRLVGSYEEPGREKGSAEDL
                     VHRGETVGRVVRTREGVSPVYVSVGNGIDLGSAVDLVLACCTRYRLPESTRQAHNA"
     gene            complement(946..2643)
                     /locus_tag="VN178_02650"
     CDS             complement(946..2643)
                     /locus_tag="VN178_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWS79898.1"
                     /translation="MSKKKTPSGMRAGIAGVVGAVVAFGIAELVHGLYSSVPSILVSI
                     AQRIVELTPGGLVTAGIELLGKADIPTLIVTVLLGTVAISFFLGNLAVRHPSLALAGV
                     AVLSAIAVAAALADPFVAPAPTIITIAGALLAGAAVTELLLRAAGLRAAIEPAEEPPQ
                     AGEPGSAASPVARAREAGSDGSIAVGRGGFLLLGGGAAVAGLAAAGVGRLLGGGTVQS
                     AARPKKLNLPEAQDEKQPAGKGEADKSAGEAATHESLPQPPADASIDVPGMPKLITPA
                     SSFYLIDTALSSPRIDVNDWTLSVKGAVDNPVEFSFQDLLGMSTREADITLSCVSNTV
                     GGGLVSNGRWTGVLLSDVLAEAGMSRDKITTASRQLVGRSVDGFTTGFKTDIALDGRN
                     ALVAFGLNGSELPVKHGYPVRLVIPGLYGYISATKWLTEIELTNWNFDAYWVQRTWSK
                     EGPIKTQSRIDTIRDGDNLSSGKNPIGGIAWAPHRGIQKVEVSTDGGVTWNTARLAKQ
                     LAEDAWRQYVYDWDARPGDYTIQVRATDGNGQTQTASKAPPHPSGATGYHTIDVTVG"
     gene            3143..>5605
                     /gene="ileS"
                     /locus_tag="VN178_02655"
     CDS             3143..>5605
                     /gene="ileS"
                     /locus_tag="VN178_02655"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWS79899.1"
                     /translation="MKFDKVSPQVDFPRLEEETLGLWRETNVFEKSVNGRPEDRPFVF
                     YEGPPTANGRPGFHHTLARAFKDLIPRYKTMQGYRVERKGGWDCHGLPVEIQVEKELG
                     LEGKGDIERYGVEEFNRLCRESVFRYVEDWKKTSERLGFWIDMDNPYWTLENSYIESV
                     WWALKELYENNLLYEGYKVSAHCPRDQTSLSSAEVALGYQQYPDPVVDPSVYVRLPLI
                     DEENTSLLVWTTTPWTLISNAAVAVGPEVQYATVEVDGERVILAEELLEKVLGQDGYE
                     VLFTTEGRNLEGKAYRRPFDYVPVEETENLWSVVLGDYVTTTEGTGLVHTAPAYGEED
                     ARTGRRYGLPTIHPVRPDGTFDGRVGPFAGQFVKDADPGLVEELREKGLLFRAEVLEH
                     AYPHCWRCGTPLLYYAKKAWYGRTTAVLDELLSENEEINWVPEHMKWGRFGDWLRNNI
                     DWALSRERYWGTPLPIWRTASGETIVVGSAEELRELAIDSVPEDLHRPYIDRVRIRHP
                     ETGEEATRVPEVLDAWFDSGSMPFAQWGYPHVEGGEERFRSQFPADYICEAVDQTRGW
                     FYSVLAVSTMLFGKSAYETCLVLGHILDAEGKKMSKSLGNVIDPWDVFEKQGADALRW
                     ALYTATSPGNTRRFSRTQVDEAVRKYLLTLWNTYSFFVTYARIDGFDPKEDYVEPEGR
                     SLMDRWALSELQLTVRTVSERLDAYDVTAAGRAVGEFVEELSNWYVRRSRRRFWKGED
                     DQDKKAAHSTLYECLVTVARLTAPFTPFIAEALYQNLVVNVSEEAPESVHLADWPEHR
                     EELVDRDLSERMSAARRVVGLGR"
BASE COUNT         1049 a         1732 c         1827 g          997 t
ORIGIN      
        1 cggcgttatg cgcctggcgc gtgctctcag gcaacctgta cctcgtgcag caggccagga
       61 cgagatccac cgcgctaccc agatctatac cgttgcccac cgagacgtag accggggaga
      121 ccccctctct cgtccttacc accctaccga cggtctcccc acgatgcacg agatcctctg
      181 cgctaccctt ctccctccct ggctcctcat agctccccac cagccggctc ttggcgcaac
      241 ccaccgtcgg aacgtcgaga aagatcccaa ggtgcgaggc caggcccatg cgacgggggt
      301 gcgcgaggcc ctgggcgtcc aggatcaggg cttccacccc ggtctccacc tcgcgcaaag
      361 ccccggccaa cgacggcact tccctgaacg aaaggagccc gggtacatac ggaaaccgga
      421 gcggcgcctc gaagccctgc acttcgacga gggagagccc aggaaactcg agcaccacga
      481 ccgtggcata ggccgtgtcg ccctgtgtag agacgtcggc cccggccaca tgccgcacgc
      541 cggagaggtc gagggccggc cccgtaacca ccctgggcgc caactcctcc tgtagccgcc
      601 tggcctcgga cggtgagaga tcaaacccgt gaagctcgtc gaccttcatc ctacgttaac
      661 aaatatgtac gcggggcgag ctcccggata cactgcctac cgataacccc cagaaaacac
      721 cgtcccgacc gatggaatct ggagggggga acggttccat cggccggggc tcggtttaat
      781 ctgaatctta cccttaacct gttacggtat gcttgccgta atgttacggt ttcgtaacgt
      841 atcggaaaca ctttggttac aggtttgttt ctctgcggcg aaacatttgt taccgccctt
      901 acatgcccga ccctaacgct atagttgaga tattgtagcg gatctctagc ccacggtcac
      961 gtcgatggtg tggtaaccgg tggcgccgga ggggtgtggg ggggctttcg aggcggtctg
     1021 ggtctgtccg ttaccgtccg tggcgcgcac ctggatggtg taatcgcctg gacgggcatc
     1081 ccagtcgtag acgtattggc gccaggcatc ttcggcgagt tgttttgcca ggcgggctgt
     1141 gttccaggtc actccgccgt ctgtcgagac ctcgaccttc tggattccgc ggtgtggtgc
     1201 ccaggcgata ccccctatgg ggttcttgcc ggaggagagg ttgtcgccat ccctgatagt
     1261 gtcgatacgg gactgggtct tgatcggccc ttccttggac caggtacgtt ggacccagta
     1321 agcgtcgaag ttccagttgg tgagctctat ctcggtgagc cacttggtgg ccgagatgta
     1381 tccgtagagg cctgggatga cgagtcggac ggggtagccg tgctttacgg ggagctcgct
     1441 gccgttcagg ccgaaggcga cgagggcgtt gcggccgtcc agcgcgatgt ctgtcttgaa
     1501 gcccgtcgtg aagccgtcga cgctgcggcc gacgagctgc ctggaggcgg ttgtgatctt
     1561 gtcccggctc ataccggcct ccgccagaac gtcggagagc aagaccccgg tccagcgtcc
     1621 gttggagacg aggcctccgc cgacggtgtt ggagacgcag gagagcgtta tgtccgcctc
     1681 gcgggtggac atgccgagga ggtcctggaa agagaactcg acggggttgt ctaccgcgcc
     1741 tttgacggag agcgtccagt cgttcacgtc tatcctcggc gaactgagcg cggtgtctat
     1801 gaggtagaag ctgctcgccg gagtaattag cttcggcatg ccgggcacgt ctatggaggc
     1861 atccgccgga ggctgcggca gcgactcgtg cgtcgcggcc tctccggcgc tcttgtcggc
     1921 ctcacccttc cctgccggct gtttctcgtc ctgcgcttct ggaaggttca gctttttggg
     1981 tctggccgcg ctctggacgg ttccaccgcc aagcaggcgt cccacgccgg cggccgcgag
     2041 accggccacg gccgcgccgc cgcctagcag caggaacccg ccgcgcccga cggcgatgct
     2101 gccgtccgag cctgcctccc tcgccctcgc gacgggcgag gccgcgctgc caggctcgcc
     2161 cgcttgggga ggctcttccg caggttctat ggcggcccgg aggcccgccg cgcgcaacag
     2221 gagttccgtg acggcggcac cggcgaggag ggctccggcg atggtgatta tggtcggcgc
     2281 cggggccacg aacggatctg ccagggccgc ggccacggct atggccgaca ggacggcgac
     2341 gccggcgagc gccagagaag ggtgtctgac ggcgagatta ccgaggaaga agctgatcgc
     2401 cacggtaccg aggagcaccg taacgataag cgtcggtatg tccgccttgc cgaggagctc
     2461 gatgcctgcc gtcacgagcc cgcctggtgt caattccact atccgctggg ctatcgacac
     2521 gaggatcgaa ggaacagagg agtagagccc gtgcaccaac tccgcgatcc cgaacgcgac
     2581 cacggctccg accacaccgg cgatccccgc gcgcatcccg cttggtgtct tcttcttgct
     2641 catggtggtg ttctccaata cctctcgctg tgagaccttc cgttgagtct atacctcgga
     2701 tggttgcttg ggcctcaaaa attcttacaa ctacattacg aatttgcttt gcggaccgga
     2761 tcatcgtccg acttgacaca accgctcttc ttcgctacaa agtctcctac aggcgatgac
     2821 gggaacgagt agggtcgcag acccccgtct gagcgagccg gggacggtgg aagcccggcg
     2881 cgggacgcgg cccgaacatc ctccccgagc cgccggccga aagcggaagg cgagagtggc
     2941 ctagacggtt agtaggaccg gccgggtgag cccgttacag cgcgcgcgtt ccgggagacc
     3001 gcgacgccac ggagaggacc ggacccgtga ggttccgggc aagtagggtg gtaccgcgag
     3061 ggccttatgc aggctcccgt ccctgcggtg gagcgagact ccgcccaggg ggcgggagct
     3121 tttgtatgag gaggtcgacg gcatgaagtt cgataaggtc agcccgcagg tagactttcc
     3181 gaggctcgag gaggagaccc tcggtctctg gagggagacg aacgttttcg agaagtctgt
     3241 aaacggcagg cccgaggaca ggccgttcgt cttctacgag gggccgccga cggccaacgg
     3301 gagaccggga ttccaccata ccctggcgag ggctttcaag gacctcatcc cccgctacaa
     3361 gaccatgcag ggttaccggg tcgagcgcaa gggtggctgg gattgtcacg gtctgccggt
     3421 ggagatccag gtagagaaag agctaggcct cgagggcaaa ggggacatcg agcgctacgg
     3481 cgtcgaggag ttcaaccgcc tgtgccgcga gtccgtcttc cgttacgtgg aggactggaa
     3541 gaagacgagc gagcgactcg gcttctggat agatatggac aacccctact ggaccctgga
     3601 gaattcatac atcgagagcg tctggtgggc actcaaagag ctatacgaga ataacctcct
     3661 ctacgaaggt tacaaggtta gcgcccactg cccccgcgac cagacctccc tctcctccgc
     3721 cgaggtagcg ctcggctacc agcagtatcc tgatcccgtg gttgacccga gcgtctacgt
     3781 gaggctgcca ctgatagacg aagagaacac gagcctcctc gtctggacaa caacgccgtg
     3841 gaccctcatc tccaatgccg ccgtcgccgt agggcctgag gtgcaatatg caaccgtcga
     3901 ggtggatggc gagcgtgtga tcctggccga ggagctactt gagaaggttc tcggccagga
     3961 cggttacgag gtgctcttta cgactgaagg acggaacctc gaaggcaagg cgtaccgccg
     4021 ccccttcgat tacgtacccg tggaggagac ggagaacctc tggagcgtcg ttctcggaga
     4081 ctacgtgacg accacggaag ggacggggct ggtgcacacc gctccggcat acggggagga
     4141 ggacgcccgc acgggccgca ggtacgggct gcccacgatc cacccggtca ggccggacgg
     4201 cactttcgac gggcgggtcg ggcctttcgc cgggcagttc gtcaaggacg cggacccggg
     4261 tctcgtcgag gaattgaggg agaaggggct cttgttcagg gcggaggtac tggagcacgc
     4321 ctacccgcac tgctggcggt gcggaacacc gcttctatac tacgcgaaga aggcctggta
     4381 cgggcggact accgcggtgc tcgacgaact tctctcggag aacgaagaga tcaactgggt
     4441 ccccgagcac atgaaatggg gtcgctttgg ggactggcta aggaacaaca tcgactgggc
     4501 cttgagccgc gagcgctact ggggtacccc gctcccgatc tggcgcaccg cctcgggtga
     4561 gacgatcgtg gtcggtagtg ctgaagagct gagagagctt gccatagact ccgtccccga
     4621 ggacctgcac aggccttaca tagacagggt gcgcataaga cacccggaga cgggcgagga
     4681 ggccacccgc gtccctgagg tcttggacgc ctggttcgac tcggggagca tgcctttcgc
     4741 ccagtggggc tatccgcacg tggagggcgg cgaggagcgt ttccggagcc agttccctgc
     4801 cgactacatc tgcgaggccg tcgaccaaac ccggggatgg ttctactcgg tgctcgccgt
     4861 ctcgacgatg cttttcggca agagcgcgta cgagacttgc ctggtcctcg ggcatatact
     4921 cgacgccgag ggcaagaaga tgagcaagtc tctcggcaac gtcatagacc cctgggatgt
     4981 cttcgagaag cagggcgccg atgccttgcg ctgggcgttg tacactgcca cgagtcccgg
     5041 caatacgcga cgcttctcca gaactcaggt cgacgaggcc gtccgcaaat acctgctcac
     5101 cctctggaac acctactcgt tcttcgtgac ctatgcccgt atcgacggct tcgatcccaa
     5161 ggaggactac gtcgagcccg agggacggag cctgatggac cggtgggccc tcagcgagct
     5221 gcagctcacc gtgaggaccg tgagcgagag actcgacgcc tacgacgtaa ctgcggcagg
     5281 cagggccgtc ggggagttcg tggaagagct ctccaactgg tacgtgagac ggagccgcag
     5341 gcgcttctgg aagggagagg acgatcagga caagaaagcc gcacactcca ccctctacga
     5401 gtgtctggtc acggtcgcca ggctcacggc cccctttacc ccgttcatag cagaagccct
     5461 ctaccagaac ctggtagtga acgtgagcga agaggctccc gagagcgtac acctcgccga
     5521 ctggcccgag cacagggagg aactcgtaga ccgcgacctc tcggagcgga tgtccgcggc
     5581 gaggcgcgtg gtcggtctag ggcgg
//
LOCUS       DATMVF010000061        11952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_330226, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000061 DATMVF010000000
VERSION     DATMVF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11952
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330226"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VN178_02660"
     CDS             <1..1681
                     /locus_tag="VN178_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWS79900.1"
                     /translation="FAAGRANGVYRTEDKVRDGERAGLDAARHVGKGESAGTKVQRHP
                     GPAPSHPYPVYDHPKGMAFVDLDEDVQLKDIKNSIQEGFDSIALVNRFATLGMGPSQG
                     KHSNMGGIRIVARLMTKTIEETGTITSRPFFHPVPMGMLAGRSFHPARRTPMHRRHEE
                     LGAVFMRAGDWFRPEYYATPNMGREDAILAEVRVVRRGVGIIDVGTLGKLEVAGPDAL
                     ELVERVCTGKFAKLAVGMTRYALMIDEAGVITDDGVCARLSEDRFYLTATTSGVDALY
                     REMTRWIQVWDLDVEVTNYSNAFAAMNLAGPLSREVLSGLTDIDLSEEAFPYLGVREG
                     RVAGIPARVMRVGFVGELGYEVHVPADGGLHVWDAITGAGEAAGIRPFGVEAQRRLRL
                     EKGHVIVGQDTDGLTNPWEANVGWAVKLDKPFFIGKRTLEILEKRDQQAERRGGEDRR
                     LIGQRTQDRKLAGFALVGGGPIPKECHLVIEDGEIIGRVTSVAHSEALGKVIGLAFLP
                     SDRAVGSRFQIRADGGEMVEAEVVKVPFYDPDNSRQKGAGQREVAYQGEKA"
     gene            1678..2361
                     /locus_tag="VN178_02665"
     CDS             1678..2361
                     /locus_tag="VN178_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79901.1"
                     /translation="MMEGTVRRSPVHDILERLDPEWGRIGSAPAALHFGDPEGEAALK
                     ERLGLCDVSALKRFGVKGPNAHRWLLDRGVEVPEPANSWSPLSDGRGLVARLGRSEFL
                     VEDGPDGYITEELGDSLNSGARGIYPVMRQDAAFALTGERAGEVMLQVCGVDFGALDY
                     EARPIVLARVAVISAVVLPRVEGETRTFRIWCNSPYGIYLWEELRKIAEEFGGGVVGL
                     SALYGGGGR"
     gene            2351..2569
                     /locus_tag="VN178_02670"
     CDS             2351..2569
                     /locus_tag="VN178_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS79902.1"
                     /translation="MEDNRRGVYGQNLVALTIESAAMSKMPLASISRGMLSKIENGQT
                     SASLSTLTKVARALARHSSIVEGRAFDT"
     gene            2677..3318
                     /locus_tag="VN178_02675"
     CDS             2677..3318
                     /locus_tag="VN178_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS79903.1"
                     /translation="MNGHEVARARPRGGLADKRQAILGGALAVFSRDGYTRASIDAIS
                     AEAGVSTRTIYNHFEDKGHLFQTVIQESTALVADAQIAIIASYLRKVTDLEEDLIEFG
                     RALATPMSDYAEHFALVRQINAEVGHIPQEAIDAWQEAGPRRVLGELALRMRRLGEQG
                     LLSVDDPDRAAVHFMLLVQGAVPFHHGIGATSEEEIEEIVTSGVRTFLHGYLP"
     gene            complement(3434..4009)
                     /locus_tag="VN178_02680"
     CDS             complement(3434..4009)
                     /locus_tag="VN178_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWS79904.1"
                     /translation="MPVFGEPRPERADAARNRRRILEAAERLVAEQGVEGLCMGDVAG
                     AAGVGVGTLYRRFGDRAGLAYALLDERTREFQEAFLSGPAPLGPGASAAERIRAFLHG
                     YVDYLLEPHIELMLMAETASPGARYRSGAYRFHHAHVSALIQEVHPDVDAEYLAGALL
                     APLAADLYVHQRREREMTTERIKVGLDELLD"
     gene            3960..4481
                     /locus_tag="VN178_02685"
     CDS             3960..4481
                     /locus_tag="VN178_02685"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWS79905.1"
                     /translation="MRAASARSGRGSPKTGKRARAALSDEPTSVRIDSTEHILAEFIP
                     FSRVRVANRTYYAPLPDPEAPAPVRFLPRFDNALLSHADQVRIVSDEYRKRLIASGGM
                     GSVGTVLVDGFVGGTWKTQRARGEVTQEIEPFEALSRKHRDAVADEGERFVRFIARPE
                     ATEAYGVRFVETN"
     gene            complement(4612..5049)
                     /locus_tag="VN178_02690"
     CDS             complement(4612..5049)
                     /locus_tag="VN178_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS79906.1"
                     /translation="MSIEDQNMAVVRRFYEGYAAGDPEKIRETLADDVTWLIPGHHPL
                     AGLKRGAEEIVAFFDQLGKANFRAEVLSLSASEDSVVDVHRGWGEHGDAKLDINWVLY
                     YRIRNGKIAEVQNFAGDQHAADLFFWEVWGQSLKPLPDRLSDA"
     gene            complement(5039..5569)
                     /locus_tag="VN178_02695"
     CDS             complement(5039..5569)
                     /locus_tag="VN178_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS79907.1"
                     /translation="MAGPSNGEGALINDTYTDHADRAEILDTIASFAQRADRRDWEGL
                     VGCFTEMVDRDYSSLTGSGPDRLPARKLVVEEWAPVLGALDATQHLIGLPAVSSDGES
                     ATATAHFQAQHVLANTDGGDRWTLGGRYDFEMRRETGGWRISGVTMTALWSEGNQRVM
                     TRAAESSGLREDDHEH"
     gene            complement(5547..6266)
                     /locus_tag="VN178_02700"
     CDS             complement(5547..6266)
                     /locus_tag="VN178_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS79908.1"
                     /translation="MNEAAGDGAASVALVTGANRGIGLEVARQLARRGMMVILGARDQ
                     EKGQAAAKGLAAEGLSVIPKQLDVADDLSVDKLAAEVEDDPGRLDVLINNAGIHYDTW
                     QRTLSADLEVVREALETNLLGAWRVCEALVPLMRRDGGGRIVNVSSGAGSLSEMGAGT
                     PAYGVSKAALNALTVRLAAELRSTGILVNSVCPGWVATDMGGSGGRPVEDGAAGVVWA
                     ATLDEDGPSGGFFRDGRPIEW"
     gene            complement(6266..7246)
                     /locus_tag="VN178_02705"
     CDS             complement(6266..7246)
                     /locus_tag="VN178_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS79909.1"
                     /translation="MSAEEPTNVMPLVDSARRDEVRFESEGLPLAGHIYRARGVPEGE
                     SAPALVMAGPMTSVKEETLPHYAERLQDAGYTVLAFDNRNFGESAGEPRQHLDTHEQV
                     EDLKNAVSYMLSREDTDPERLGLCCVCLGAGYGLEVAAMDRRIKAVALVAGGYNITDT
                     YLGFLGSDGFREYIENLGVARQHQYETGEIQYMPAVAGPPDYAPSAMPVEEAYAYYTR
                     AQEREAPNWENRLTLASMEHIVGWKVIGHAHLVEQPLLVVHGTTDVLLPPRYAQEVYE
                     RAPEPKEVSWIETTNHVELYDGVPHVPQALERIVPWLDENLKSRAGEGSA"
     gene            complement(7418..8293)
                     /locus_tag="VN178_02710"
     CDS             complement(7418..8293)
                     /locus_tag="VN178_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWS79910.1"
                     /translation="MRSRWRKRVGSEEGQHIVRAWSQGGVLLESYRYAPGHAAETPRH
                     SHGDYQLCFSPNFPGEYRYRGESHAVPVGSLSVIHPGEIHSAHDPQDREIPAEYRMLY
                     ARPSVFQRVVTEIAGHEGGLPSFPVPVVLDEHLARSFAGMHTALEGSASALEKDSRLL
                     SVLSEFVVRWASERLSPHRIGNEPRAVALAREYLEDDPAGNVSLEELSRIAHLSSYHL
                     CRVFKKEVGIPPHAYQINVRLDRAKDLLLGGWTISEVAYRTGFYDQSHFTHSFKRLVG
                     VTPGNYAKNSKIFQD"
     gene            8819..8959
                     /locus_tag="VN178_02715"
     CDS             8819..8959
                     /locus_tag="VN178_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79911.1"
                     /translation="MVVGEGQPDPHKVGLQTLKELAEAAGLDFERRTGGPFGYFVGFE
                     AL"
     gene            9098..9352
                     /locus_tag="VN178_02720"
     CDS             9098..9352
                     /locus_tag="VN178_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWS79912.1"
                     /translation="MGVLSWIVFGLIAGIVAKLLMPGRDPGGCIITMILGVAGAFVGG
                     FIYRLVTGTELLYFGFDLRSFAVAILGAVAVLAVYRLILR"
     gene            9349..9591
                     /locus_tag="VN178_02725"
     CDS             9349..9591
                     /locus_tag="VN178_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79913.1"
                     /translation="MSQGRNENLNLLPPKEGFKLYRYKNAAKMVTSGWSGLYWARKNE
                     AGDYEIRAVTAEGEGYSYPGGVFAKEAFERYYEKAD"
     gene            9588..10580
                     /locus_tag="VN178_02730"
     CDS             9588..10580
                     /locus_tag="VN178_02730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWS79914.1"
                     /translation="MITLALTGDVMLGRGVTDVLDDHMRPEAPWGDVMPLLDAADLRI
                     INLECAITEYERPWTRTPKVFHFRAKPSAVEILRKARIDACSLANNHTLDFEEQGLLD
                     TLEHLDAAGIRHAGAGRNRAEAADPAILTVPADHTSQVALLAFTDNEPPFAAGIDRPG
                     TNYLPVSSKPDVLRRVERAVSYVRDMGADTVIFSNHWGPNMVQRPKETFRRFARAVID
                     RGVDIYYGHSAHVFQGVEIYRGKPILYDTGDFIDDYAVDPEMRNDWSFLFRVSVEGDT
                     FGRLDLTPVKLSYAQVDLATGDEREIILDRMERLSAEMGTVFARREGALVLERG"
     gene            complement(10667..11302)
                     /locus_tag="VN178_02735"
     CDS             complement(10667..11302)
                     /locus_tag="VN178_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HWS79915.1"
                     /translation="MSEPEDLVRTIIDEGVGDPRLLEALRAVPRAGFVPANLAGQAYE
                     DRPLPIPRGQVTTQPSLVARMVEALGLAGPERVLEVGAGYAWQTALLARLGGFVWAVE
                     RFGDVADAARENLARFGVTNAEVVEGDGTEGLPEHAPYDAILVAAAFPTVPRPLEEQL
                     AAGGRLVQPVGHGGREEVVLFRKGARGLVRRRTIAWARFVRLHGAHAFPEQ"
     gene            11595..>11952
                     /locus_tag="VN178_02740"
     CDS             11595..>11952
                     /locus_tag="VN178_02740"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWS79916.1"
                     /translation="MRETAEPQILLSGLAIGESPRWHEGRLWFSNWGTQEIVAVDLEG
                     NSEVMARVPTTIPFSIDWLPDGRMIVVSGPEARLLRREPDGTLAIHADLSGLVEGFIN
                     EIVVDSHGNAYVNGGDF"
BASE COUNT         2120 a         3847 c         3906 g         2079 t
ORIGIN      
        1 cttcgccgcc ggacgcgcca acggcgtcta ccggaccgag gacaaggtac gcgacggcga
       61 gcgcgccggg ctcgatgccg cccgccacgt cgggaagggc gagtcggccg ggacaaaggt
      121 gcaacggcat cccgggccgg cgcccagcca cccctatccg gtctacgacc acccgaaggg
      181 catggccttc gtggacctcg atgaggacgt gcagctcaag gacataaaga attccatcca
      241 ggaagggttc gacagcatcg cgctcgtcaa tcgcttcgcc actttgggga tggggccgag
      301 ccagggcaag cactcgaaca tgggcggcat ccgcatcgtg gcccggctga tgaccaagac
      361 catcgaggag acaggcacga tcacctcccg ccccttcttc caccccgtgc cgatgggaat
      421 gctggccggg cgcagcttcc atcccgcgcg ccgcaccccg atgcaccgcc gccacgagga
      481 actcggcgcg gtcttcatgc gcgccgggga ctggttcagg ccagagtact acgccacgcc
      541 caacatgggg cgtgaagacg cgatactagc cgaggtccgg gtggtccggc ggggagtcgg
      601 catcatagac gtcgggacgc tgggcaagct ggaggtcgcg ggtcccgatg cgctggagct
      661 cgtcgagcgg gtctgcaccg ggaagttcgc gaagctggcg gtcgggatga cccgctacgc
      721 cctgatgatc gacgaggccg gggtgatcac ggacgacggc gtgtgcgcaa ggctctccga
      781 ggaccgcttc tacctcacgg cgaccacctc gggagtggac gccctctaca gggagatgac
      841 gcggtggatc caggtatggg acctcgacgt cgaggtcacg aactacagca acgccttcgc
      901 cgccatgaac ctcgccggcc cgctctcgcg ggaggtcctc tccggcctga ccgacatcga
      961 cctctcggag gaggcgttcc cttacctcgg cgtccgtgag ggccgggtcg ccgggattcc
     1021 ggcccgggtc atgcgcgtcg gcttcgtcgg tgagctcggc tacgaggtgc acgtccccgc
     1081 cgacggaggg ctacacgtgt gggacgccat cacgggggcc ggagaggccg ccggcataag
     1141 gccgttcggg gtcgaagccc agcggcgcct gcgcctcgag aaggggcatg tcatcgtcgg
     1201 tcaggacacc gacggcctca ccaacccctg ggaggcgaac gtgggttggg ccgtgaagct
     1261 cgacaagccc ttctttatcg ggaagcgaac gctcgagatc ctcgagaaga gggatcagca
     1321 agcagagcgg cgcgggggcg aggaccggcg cctgatcggc cagaggaccc aggatcgtaa
     1381 actcgccgga ttcgccctgg tgggcggagg ccccatcccc aaggagtgcc acctggtcat
     1441 agaagacggg gagatcatcg gccgggtgac cagcgtcgcc cacagcgaag ctttgggcaa
     1501 ggtcataggg ctggcgttcc tgccctcgga cagggccgta ggctcgcgat ttcagattcg
     1561 ggccgacggc ggggagatgg tcgaggccga ggtggtgaag gtcccgttct acgacccgga
     1621 caactcccgc cagaagggcg ccggtcaaag ggaggtcgcc taccagggag agaaggcgtg
     1681 atggaaggga ccgtcaggcg cagcccggtg cacgacatcc tggaacgcct ggaccccgag
     1741 tggggacgga tcggctcggc gcctgccgcc ctccatttcg gagaccccga gggagaggcc
     1801 gcgctcaagg agcgccttgg cctctgcgac gtgagcgccc tgaagcgctt cggcgtcaag
     1861 gggccgaacg cccaccggtg gctcttagac cgtggggtag aggtaccgga gccggcgaac
     1921 agttggtcgc ccctctccga cggtcgaggc ctcgtcgccc gcctggggag aagcgagttc
     1981 ctggtggagg acggtcccga cgggtacatc acggaggagt tgggcgattc gctgaactcg
     2041 ggggcgaggg gcatctaccc ggtgatgcgc caggacgcgg ccttcgcgct gacgggcgag
     2101 cgggccgggg aggtcatgtt gcaggtctgc ggcgtggact tcggggcttt agattacgag
     2161 gcgcgtccca tcgtgctggc gcgcgtcgcc gtgatctcgg cggtcgtgtt gccgcgggtg
     2221 gaaggagaga cccggacctt caggatctgg tgcaactccc cctacgggat ctacctctgg
     2281 gaggagttgc gcaagatcgc ggaggagttc ggcggtggag tggtggggct ctcggcactt
     2341 tacggcgggg gtggaagata accggagagg cgtctacggt cagaacctgg tggcgctcac
     2401 catcgagtcg gccgccatgt cgaagatgcc cctggccagc atcagccgcg ggatgctcag
     2461 taagatcgag aacgggcaga cctccgccag cctgagcacg ctgacgaagg tggctagggc
     2521 gcttgctagg cactcctcta tagtggaggg cagagcattc gatacatagt gcagggggac
     2581 ggttttcggc ttgcaccttc gtacggtcta catggtaaag caccacgtgc acggcgaaag
     2641 gtaaaatgac cattgtagag tctaagaggg ttgaagatga acgggcacga agtggcgagg
     2701 gcgcggcctc gtgggggatt ggcggacaag cggcaggcta tcctcggcgg tgctcttgcg
     2761 gtgttctcgc gggatggcta cacccgggcg agcatagatg ccatctccgc cgaggccggc
     2821 gtctccacgc gcaccatcta caaccacttc gaggacaaag ggcacctctt ccagacggtc
     2881 atccaggaga gtacggcgct ggtcgcggac gcccagatcg cgatcatcgc cagttacctg
     2941 cgcaaggtca ccgacctgga ggaggacctg atcgagttcg gccgggccct ggcgacgccg
     3001 atgagcgact acgccgagca cttcgcgttg gtgcggcaga tcaacgccga ggtcgggcac
     3061 atcccgcaag aagcgatcga cgcgtggcag gaggccgggc cgcgccgggt gctgggtgaa
     3121 ctcgccctcc gcatgcggcg gttgggggag cagggactgc taagcgtcga cgatcccgat
     3181 cgggcggccg ttcacttcat gctgttggtc cagggcgccg ttccgttcca ccacggtatt
     3241 ggcgccacca gcgaggagga gatcgaggag atcgtcacct ccggcgtccg caccttcctg
     3301 cacggctacc tcccctgaag gttaccgact gctgggcgca acggtagaca tcggcgagac
     3361 attcgcagtc gtcaatccta ccgacgcacc cagaagatgt gtgcgaagca tgatctagcg
     3421 tagataatcc cggtcaatcg agtagctcgt caaggcccac cttgatccgc tcggtcgtca
     3481 tctctcgttc gcgcctttgg tgcacataga ggtcggcggc caatggagcc aacagcgctc
     3541 cagccaggta ttcggcgtcg acgtccggat ggacctcctg gatcaaggca gacacgtgcg
     3601 cgtggtggaa tcggtaggcg ccagagcggt agcgggcgcc cgggctcgcg gtctccgcca
     3661 tgagcatcaa ctcgatgtgc ggctccagca gataatctac gtacccgtgt aagaacgccc
     3721 ggatgcgctc ggcagcagac gcccccggcc ccagcggcgc gggaccgctc aagaacgcct
     3781 cctggaactc gcgcgtacgc tcgtccaaaa gcgcatacgc cagcccggcg cggtccccga
     3841 aacgcctgta caacgtaccc acgcccaccc ccgcagcccc ggctacatcg cccatacaca
     3901 gaccctccac accttgctcg gccaccagac gctcggcagc ctccaggatc ctgcgccggt
     3961 tgcgggccgc gtcagcccgc tccggtcgcg gctctccgaa gaccggcaag cgtgctcgcg
     4021 ctgcgctatc ggacgagccc acttctgtgc gcatcgattc cacggaacat attctagcgg
     4081 agttcattcc gttttcccgt gttcgggttg caaacagaac gtattacgcc ccgctaccag
     4141 accctgaggc tccggctccg gtgcgcttcc tgccccggtt cgacaacgcg ctgctatctc
     4201 atgccgacca ggtccgcatc gtcagcgacg agtatcgcaa gcggctcatc gccagcggcg
     4261 gcatgggctc cgtcggcacc gtgctcgtag acggtttcgt cggcgggacg tggaagacgc
     4321 agcgcgctcg cggtgaggtt acacaggaga tcgaaccgtt tgaggcctta tcaagaaagc
     4381 accgcgacgc cgtagccgat gaaggggagc gattcgtccg cttcattgca aggccggagg
     4441 ctaccgaagc atacggggtc cggttcgtcg agacaaactg aatcagatac caagggacct
     4501 ctttccaacc cttgctcaac gaagcctaag gatggttcgc aggccctcgc tagggtactg
     4561 ttcgccccag cttcgggttt gcagaccacc tggtccctgc gtccaaggaa gttatgcgtc
     4621 ggagagccgg tcggggagtg gtttgaggct ctggccccag acctcccaga agaagaggtc
     4681 ggcggcgtgc tggtcgccgg cgaaattctg cacctcggct atcttgccgt tcctgatacg
     4741 gtagtagagc acccagttga tatccagctt cgcatctcca tgctcgcccc agccgcggtg
     4801 gacgtctacc acggagtcct ccgaggcgct cagggagagg acctcggccc ggaagttggc
     4861 cttgccgagc tggtcgaaga aggccacgat ctcctccgct ccccgcttga gacccgcgag
     4921 agggtggtgg cctggaatga gccaggtgac gtcatcggcg agggtctccc taatcttctc
     4981 cgggtcaccg gcagcgtaac cctcatagaa ccgccgcacc accgccatgt tttggtcctc
     5041 aatgctcatg atcatcctct cgtagtcccg agctctctgc cgcccgggtc attactctct
     5101 ggttcccttc gctccacagg gccgtcatcg tgacgcccga gatcctccag ccgccggtct
     5161 cgcggcgcat ctcgaagtcg tagcgtccgc cgagcgtcca ccggtcgccg ccgtccgtgt
     5221 tcgcgagaac gtgctgcgcc tggaagtgag ccgtcgccgt ggcgctttcg ccgtccgacg
     5281 agaccgcggg cagtcctatg aggtgctggg tcgcgtccag agccccgaga accggcgccc
     5341 actcctctac gaccagcttt cgggcgggga ggcggtcagg acccgagccg gtgagcgagg
     5401 agtagtcccg atcgaccatc tccgtaaaac acccgacgag cccttcccag tcgcgccgat
     5461 cggcccgctg ggcgaagcta gcgatcgtgt ccagtatctc ggcccggtct gcatggtccg
     5521 tgtaggtatc gtttatcaaa gctccctcac cattcgatgg gcctgccatc acggaagaat
     5581 cctcccgagg ggccgtcttc gtcgagggtg gcggcccaca ccacgcctgc ggccccatcc
     5641 tctacgggac gtccaccgga tccgcccatg tccgtcgcca cccacccggg gcacaccgag
     5701 ttcaccagga tgcccgtgct cctgagctcg gcggcgagcc ttacggtcag ggcgttcagg
     5761 gccgccttcg agacgccgta ggcaggggtg cccgcgccca tctccgagag cgaaccggcg
     5821 ccgctggaga cgttcacgat ccgtcctccg ccgtccctgc gcatcaaggg cacgagcgcc
     5881 tcgcagaccc gccaggcgcc gaggaggttg gtctcgagtg cctcgcgcac cacctcgagg
     5941 tcggccgaaa gcgtccgctg ccacgtgtca tagtggatgc cggcgttatt gatcaggacg
     6001 tccagacgtc ccggatcgtc ctccacctcg gcggccagct tatcgacgct gagatcgtcg
     6061 gcgacgtcga gttgcttggg gatgacgctg agtccctccg ccgcgagccc cttcgccgcc
     6121 gcctggccct tctcctgatc gcgggctccg agtatcacca tcattccgcg cctcgcgagt
     6181 tgacgggcga cctcgagccc gatgccgcga ttggccccgg tgaccagggc gacgctcgct
     6241 gctccgtcgc cagccgcctc attcactacg cgctcccctc gccggcgcgg gacttcaggt
     6301 tctcgtcgag ccagggcacg atcctttcca aagcctgggg gacgtggggc accccgtcat
     6361 agagctcgac gtggttggtg gtctcgatcc aggagacctc cttcggctca ggcgcccgct
     6421 cgtagacctc ctgcgcgtac cgggggggta ggagcacgtc agtggtgccg tggacgacga
     6481 gcagaggctg ctccacgagg tgggcgtggc cgatgacctt ccagcccacg atgtgctcca
     6541 tcgaggccaa cgtaagacgg ttctcccagt tgggcgcctc gcgctcttgc gcgcgggtgt
     6601 agtacgcgta tgcctcctcc accggcatcg cagagggagc gtaatcgggc gggccggcca
     6661 ccgccggcat gtactggatc tctcctgttt cgtactggtg ctggcgggcg acgcccaggt
     6721 tctcgatgta ttccctgaat ccatcggagc caagaaaccc gaggtatgtg tcggtgatgt
     6781 tgtagccccc ggccacgaga gctacggcct tgatgcgccg atccatagcg gccacttcca
     6841 gcccgtaccc ggcccccaga cacacgcagc acagcccgag cctttccggg tccgtgtcct
     6901 cgcgcgagag catgtaagag acggcattct tgaggtcctc gacctgttcg tgggtgtcga
     6961 ggtgttggcg gggctcacct gcggattccc cgaagttgcg gttgtcgaag gcgaggacgg
     7021 tgtagccggc gtcctggagg cgctcggcgt agtgggggag ggtctcttcc ttgacgctgg
     7081 tcatcgggcc ggccatcacc agggccggtg cgctctcgcc ttcggggact ccccgagctc
     7141 ggtagatgtg tccggcgagc gggaggcctt cgctctcgaa gcgcacctcg tccctcctgg
     7201 cggaatcgac gagtggcatc acgttggtcg gttcttcggc gctcaaggct tctggctcct
     7261 ttctccaatt gcgggcatct ctatgggtgg ctaaacggat gtcacttcta cgatctcggt
     7321 ccgaccgagc attagcccaa gttctccctt gccgactttc ccgcaagctc cttcctcacg
     7381 tgctgacaca gcccggagta tgtagcggcc gggatcctta gtcttggaag atcttgctgt
     7441 tcttggcgta gtttcctggc gtgacgccga cgaggcgttt gaaggagtgg gtgaagtggc
     7501 tctgatcgta gaagccggtt cgataggcga cctcggagat cgtccagccc ccgagcagaa
     7561 ggtcctttgc cctgtccagg cgcacgttta tctggtaggc gtgaggcggg atgccgacct
     7621 ccttcttgaa gacccggcag agatggtaag aactgagatg ggcgatccgc gatagctctt
     7681 ctagcgagac atttccggcc ggatcgtcct ccagatactc tctggccagc gctacggccc
     7741 gcggttcgtt tccgatacga tgcggcgaga gacgctcgct ggcccagcgc acgacgaact
     7801 ccgagagcac ggacaggagg cgcgaatcct tctccagggc ggaagccgat ccttcaaggg
     7861 cagtgtgcat gcctgcgaag ctcctggcta ggtgctcgtc gaggacgacg gggaccggaa
     7921 aggagggtaa tcccccctca tgtccggcga tctccgttac cacccgctgg aagacggaag
     7981 gtctggcgta gagcatccgg tactctgcgg ggatctcccg gtcttgcgga tcgtgggccg
     8041 agtggatttc gcccggatgg atgacgctga ggctccccac gggcaccgcg tggctttctc
     8101 cccggtagcg atactcgccg gggaagttcg ggctgaaaca caactggtag tcaccgtgag
     8161 agtgtctggg cgtttctgcc gcatgacccg gtgcgtaccg gtacgactcg aggagcactc
     8221 ccccctgcga ccacgctctt acgatatgct gtccttcttc gctacccacg cgtttccgcc
     8281 aacgacttct cacgaaacac cgtcggcatg gctgacttcc ccgtctccag gctcatcggc
     8341 cgattctaca cttcgcccca actcgatgtc cgcccaccat acattcgaac gtctagcgca
     8401 acgcatctct tctccgtcga cccttggacg gcgcttcatc gagatccgag gtcgggccga
     8461 aacttacacc gacggtggag aagaggtcgg caagcgcatc aacgcgaaca agccgttcga
     8521 ttccgcgtct attcgcatct gcccgcgccg gatcctgacg atgggcatcg acggcgaccc
     8581 gttcgagctg tccgcgcgtg acgtagcgta aacgcggaac gacccgcagg cccatgttcg
     8641 aaccaaaggc cgaagaggac agatgacctg gatcgccatc ggtagcatcc gtctacggcc
     8701 gcgccccggc ggccgtagac gcgatccacg aatgtgcata ccgggtcgcg acgctgggag
     8761 aggtccctga caagcggcgc gcgaagaact cgtccgcgtc ctgaagccgg gaggccggtt
     8821 ggtggtcggc gaggggcagc cggatcccca caaggtcggt ctgcaaaccc tgaaggagtt
     8881 ggccgaggcg gcgggactgg atttcgagcg ccgaaccggc ggccctttcg gttacttcgt
     8941 cggcttcgag gctctctgac agtgccatgg ctcgaatcgt ctttagcacc gagcgcaggt
     9001 cgaggatgcg gtaggccgac ctcgggtagg ttgcatgcta gtacaatcta tctgcaatga
     9061 cagccgaagg tgcgcaggca ccacgaggag tagaagattg ggtgtgctta gctggatcgt
     9121 cttcgggctg atcgcgggta tagtcgcgaa gctgctgatg ccgggccgcg accccggcgg
     9181 gtgcataatc actatgatcc tcggagtggc tggcgccttc gtaggaggtt tcatctacag
     9241 gctcgtgacc gggacggagt tgttgtactt cgggttcgat ctcagaagct tcgcggtagc
     9301 catcctggga gccgtggccg tactggccgt ctaccggctc atcctaagat gagccaggga
     9361 cgcaatgaga acctgaacct gctgccaccc aaggagggct tcaagctcta ccgctacaag
     9421 aatgccgcga agatggtgac aagcggatgg tcggggctgt actgggcgag gaagaacgaa
     9481 gcgggagact acgagatccg ggccgtcact gcggagggag aggggtactc ttaccctggg
     9541 ggcgtgttcg caaaagaagc cttcgagaga tactacgaga aggctgattg attaccctgg
     9601 ccctgaccgg cgacgtcatg ctcgggcgcg gggtcaccga tgtcctcgac gatcacatgc
     9661 gtcccgaggc gccgtggggc gacgtgatgc cccttctcga cgcggctgac ctgcgcataa
     9721 tcaacctgga gtgcgctatc acggaatacg agcggccgtg gacccgtacg cccaaagtct
     9781 tccacttcag ggccaaaccc tccgccgtag agatcttgcg gaaggcccgg atcgatgcct
     9841 gttcgctggc caataaccac actctagact tcgaggaaca ggggttgctc gataccctgg
     9901 agcatctgga cgcggccggc atacggcacg ccggagcggg ccgcaaccgg gcggaggccg
     9961 cagatccggc catccttacc gtgccggcgg accacaccag ccaagtggcg ctgctggcgt
    10021 tcacggacaa cgagccgccc ttcgcggccg gcatcgaccg gccgggcacg aactacctgc
    10081 cggtctcctc gaaaccggat gttcttcggc gggtggagag ggctgtgtcg tacgtgcgcg
    10141 acatgggggc agacacggtc atattctcca accactgggg cccgaacatg gtgcagcggc
    10201 ccaaagagac cttccgccgg ttcgcccgtg cggtcataga tcggggggta gacatctact
    10261 acgggcacag tgcgcacgtc ttccagggag tggagatcta ccgcggcaag cccatccttt
    10321 acgatacggg cgattttatc gacgattacg cggtcgaccc cgagatgcgt aacgactggt
    10381 ccttcctctt tcgggtctcc gtagaaggag acaccttcgg gcggctggac cttaccccgg
    10441 tcaagctctc atacgcgcag gttgacctcg ctacgggcga cgagcgagag atcatcctgg
    10501 accggatgga acgcctgtcc gccgagatgg gtaccgtctt cgcccgtcgt gaaggcgccc
    10561 tggtgctcga acgcggctaa cacccccttc cgaggtcaga agtacagccc caggctcgaa
    10621 gagccgagta ggtgatacga actctccaac tggtgcccac gaacgtctac tgctccggga
    10681 acgcgtgcgc gccgtggagc cggacgaagc gggcccaggc tatagtgcgg cgacgcacaa
    10741 gccctcgagc gcccttccgg aagaggacca cctcctcccg gccgccatgg cccacgggct
    10801 gcaccaggcg tcctcccgca gcgagctgtt cctcgagcgg cctggggacc gttgggaaag
    10861 ccgcggcgac caggatagcg tcgtagggag cgtgctcggg tagtccctcg gtaccgtcgc
    10921 cctcgactac ctcggcattc gtcacgccga aacgtgcgag gttctccctc gcggcatccg
    10981 ccacatcccc aaagcgctcc accgcccaaa cgaagccgcc caggcgggcg agcagcgccg
    11041 tctgccacgc gtagccggcg ccaacctcga gcacccgttc cgggccagcg agccccagag
    11101 cctccaccat cctcgccacc agcgagggct gagtcgtcac ctggccgcgg gggatgggta
    11161 gcggcctgtc ctcgtaggct tgcccggcga ggttggcggg gacgaagcct gccctgggga
    11221 ccgcgcgcag ggcctcgagg agacggggat ctcccacgcc ctcgtcgatg atggttcgga
    11281 cgagatcctc aggttcgctc aagatcttgc catactacac tagcgttccg tccaccttcg
    11341 ccccggtctg cgggtggcct taagcctcga tccccggcag tggcagatca tggctgaccg
    11401 ccgctgattc ttcagtccgc cgcaaccgcc aagacgcctg ctgtgcaatc gctcggatca
    11461 atcatgtgtg tgcgacgcat ggccgtcacg acgctagagt gaaccgggcc gcgaatggta
    11521 cgactccgtg tgcaccgcgg gtagtcgggt ggtatcgtgg gcaagcataa gaaagccacg
    11581 gggagaggga aacgatgagg gaaacagcag aaccacagat cctgctgagc ggactcgcga
    11641 tcggcgagtc gccgcgttgg catgaggggc gtctgtggtt ctccaactgg ggtacgcaag
    11701 agatagtcgc ggtcgatctc gagggcaaca gcgaggtgat ggcccgcgtc cccacgacca
    11761 tcccgttctc catcgactgg ctgcccgatg gccggatgat cgtcgtatcg ggaccggagg
    11821 cgcgactgct gcgccgcgaa ccggacggga cgctggcaat ccacgccgac ctgagcggcc
    11881 tggtcgaggg cttcatcaac gagatcgtcg tcgactccca tggcaacgcc tacgtcaacg
    11941 gcggcgactt cg
//
LOCUS       DATMVF010000062        12609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_330350, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000062 DATMVF010000000
VERSION     DATMVF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12609
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330350"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VN178_02745"
     CDS             <1..535
                     /locus_tag="VN178_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79917.1"
                     /translation="PGLISGPHDPTDRFTYWPRRIDAGGEVLAPDRPERVVQFIDVRD
                     LAAWIIEMAAERRTGTFNATGPDHKLEMGQLLEACKAVGGDAELIWVSEDFVEEHGVE
                     PFTELPLWVPRKNAALLDIDCGRAIEAGLTFRSLSETIEDVLEWDRARPVDTESAAGL
                     TPEREQELLRAWRGVTL"
     gene            544..963
                     /locus_tag="VN178_02750"
     CDS             544..963
                     /locus_tag="VN178_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79918.1"
                     /translation="MRDEMNLTHEYMHHRTGYRLGAGCCWIRIYKGAECDAPVVVCEE
                     LPRVGDVATREVSGYLAAEVVREHFSGGLPDLPRPMLWIEHRPARRRGPGKFFLHTFP
                     SYRPRTVGAGFVRRVTLGTSRREPLDPAEVAVLIREV"
     gene            972..1166
                     /locus_tag="VN178_02755"
     CDS             972..1166
                     /locus_tag="VN178_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79919.1"
                     /translation="MGEEKGTTEYAIKQVGDRYYPVIIDKDAGGHYEINNPLTGGVLS
                     YNNPEAAEKYIQRAREKERS"
     gene            1324..2244
                     /locus_tag="VN178_02760"
     CDS             1324..2244
                     /locus_tag="VN178_02760"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWS79920.1"
                     /translation="MERTESRVGLKGVLAAVLTPMDKDLNPDHQAFAAHCQRLLAAGC
                     HGLSVFGTTGEANSLSVDERLAALEALVESGVAPEKVLPGTGSCALTDTVRLSQAALE
                     AGTAGALVLPPFYYKGVGDDGLFRFFAEVVERVGDDSLRLHLYHIPQMTGVDLGLPLI
                     SRLIDAYPTIVVGTKDSSGDRERIMTLCREFPDFSVLAGTETLLLETLRSGGEGCISA
                     TVNVTSRLARRVHDAHAAGKADEAEALQERLTHLRASIEAYPVIPALKQLMTRLTDDE
                     QWRNIRPPLSGLDEKRTKDLLSNVPLAELL"
     gene            complement(2279..4273)
                     /gene="ligA"
                     /locus_tag="VN178_02765"
     CDS             complement(2279..4273)
                     /gene="ligA"
                     /locus_tag="VN178_02765"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWS79921.1"
                     /translation="MARSVDVRGRVEDLREQVRYHNRRYHVDDAPEISDAEYDALYRE
                     LEALEDDHPELITPDSPTQRVGGEPIEGFEEVRHSVPMLSLANARKIEDLREWDARVR
                     RLLGEDETPRYVTELKIDGLAVSLRYENERLVRGATRGNGVIGEDVTAQLRTIRSIPD
                     RLDDDPPGVLEPRGEAYIPIADFEELNRELEAEGKKPYANPRNLAAGSIRQLDPRITA
                     RRPLTILLYGVGEGGEIFESHSEMLAALKAYGLRVNPYEIHQDIDTVIEECERWARKR
                     ESIGYQIDGVVVKVDSREQQLALGSVARSPRWAIAYKFEPLAGRTRLLDIIVKVGRTG
                     ALTPQAVLEPVNVGGVVISRATLHNEDYVKEKGILVGDTVVVERAGDVIPQVVRAVTE
                     ERKGDEYAFEMPANCPVCDEPVSRPEGEAVTRCVNARCPAQALEHIIHWSSKSAMDID
                     GLGEKLATRLFDLGFISDAANVYDLKAEQLVPLEGFGEKSAENLIQAIQKSKERPFSL
                     VLYALGIRHVGSVTASLIATRFGGEDFMRGVEVERLTEIDGVGEVVARAVVEYFDLED
                     NRDLVRRLMERGLDFGREEGGPAEGPLSGKRVVITGTLDRPRNYYVGRLEAAGGTFTS
                     SVSKNTDYVLAGEDAGSKLERARELGVPILDEVGFEELFS"
     gene            complement(4337..4642)
                     /locus_tag="VN178_02770"
     CDS             complement(4337..4642)
                     /locus_tag="VN178_02770"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWS79922.1"
                     /translation="MERANHRPDNPYTQLEGYRVDDASGEEVGEIEDTVYDAPSDVLK
                     YVVVGGRPIPAERIEVDAEDQRVSVPYNARTIETAPQMKDLSGAFDEAIHEHYEGQT"
     gene            4694..5668
                     /locus_tag="VN178_02775"
     CDS             4694..5668
                     /locus_tag="VN178_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79923.1"
                     /translation="MGDRTDRGRNARSLKAFSILLLALLVASLLFVHTASYEPAVSYI
                     LLYFASSVGIITAAFVVPLLPEEQFTAGLCGWIYALYAAVVAMMVFFSGGTSSELYVM
                     FFPLLLASALHGSWRLVLAVLAAVLFCHALAMLPDLLERGTGQEIASLVFYRLAALGL
                     TGLFLIYVAKSLLPAGIDEDYAMDEDGSILLGRIEEELATRGEAPVAVVLVDPGAELE
                     AMDLLLERVWSRIGEPVLLGEGNLFGVVVSGVDDGEVESAARRVLAAASSLGAQDTRA
                     GAAIYPRDARSAEDLLIAAGRALEAAFEGESPGAIVLAGSASKSARSG"
     gene            5894..6646
                     /locus_tag="VN178_02780"
     CDS             5894..6646
                     /locus_tag="VN178_02780"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HWS79924.1"
                     /translation="MGRFTRAIRGFFGRFLSGVEQRNPEILLENAVQDELENLKKLQV
                     AAARTMAYEKMLANDAVAKQKTLAVKERQARELASRGQREAAIEVVQQKQEAQASLAD
                     IESRLEDARKNSQELEQAFRDQERRYNNVVRERAALMEEHQRSRALRTANEARSNISL
                     SDSSRDLEKARQSIRQASYEAEAIGELGTSETERQIAAAEIDIKRLDAERELEMMEVQ
                     MGLREPEPIGPAPTEETASPQAEETEEGPRGQ"
     gene            6636..7427
                     /locus_tag="VN178_02785"
     CDS             6636..7427
                     /locus_tag="VN178_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79925.1"
                     /translation="MDNRKNLSRRLMVRLAPFATFAGLGTFAAFGEAYVLAGLLGIGA
                     FVTGRRLLDRRENARKELLSRVRHNRWELGRVAREDRIAASQMKRLIALQDGLLESWE
                     LLPEECRPLLDEDIFTIVGEIEGAARLARRRAALRRHLESVDRDSIHGRIQELQNDLT
                     RLEPESPMRAPFESALQGRRGELAGYHDVLGGISMINAQLESAESLLGNLRGELLALD
                     DSIVSGSGDPGLARLKERVALFRHGLDEVTRRVESLPATERISTR"
     gene            7424..8089
                     /locus_tag="VN178_02790"
     CDS             7424..8089
                     /locus_tag="VN178_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79926.1"
                     /translation="MNEDRNTARQSFFSRVFTGNTEAEGTSGTSIYSSEEIGMPGTEE
                     AEGLAAHGFTVERAAEVIRNLPPEVPRPAAVRIVRGTLEAAGIDIAELETSTRARESR
                     LNSEIDLSENRIQQFKDETEEVIRNLEDQIRKAREARDFGVSEEERKISSAEAGLDNI
                     DLVRDFFGLPQEETDEPPTAGEETQVIEGVEEEEDDTQVLRRPGPLSDSEDYLEPGDE
                     KEQ"
     gene            8086..8544
                     /locus_tag="VN178_02795"
     CDS             8086..8544
                     /locus_tag="VN178_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWS79927.1"
                     /translation="MSIPRRLGRLAQGFVSSLREDERFRERLRTGREHSEALRGAFEA
                     AWRGASEEWRKAEERRAAQEATAEQTWGWRSATTTFTPRKYPPNVLAAYDRLGLVPSA
                     SMEEVDRKRRELVKRFHPDRFSDPEKRLRAERVTAEINAAHDAIERYEAG"
     gene            complement(10158..10436)
                     /locus_tag="VN178_02800"
     CDS             complement(10158..10436)
                     /locus_tag="VN178_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79928.1"
                     /translation="MATDKDKGLVGGAADIVGGAADSVRETTSAGLGGGGPWHSANSD
                     VYHNNPNCQTGNSIAPENVQKGTGGKTLCEECERLNSAGGPVGNLTGL"
     gene            complement(10590..10823)
                     /locus_tag="VN178_02805"
     CDS             complement(10590..10823)
                     /locus_tag="VN178_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79929.1"
                     /translation="MDKAMGKAKEAEGRAEQRRAAAEAEAEQRKKTRQAEAEAKQAEK
                     ERDRQRLKDKGPLGGITDATEHVGGITDKLTGR"
     gene            complement(12265..>12609)
                     /locus_tag="VN178_02810"
     CDS             complement(12265..>12609)
                     /locus_tag="VN178_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWS79930.1"
                     /translation="AELDREGILRVRGSGLPWIAGSNTFITGHALGFPRTRVPYVFYN
                     LDKMKPGDEIFVEDKEGQEYTFRVYDLMTVEPNDYWATYPVEGKTVVSLQSCTPIPTF
                     ENRLIVRAELVS"
BASE COUNT         2318 a         3948 c         3965 g         2378 t
ORIGIN      
        1 tcccggtttg atctcgggcc cccacgatcc cacggaccgt ttcacgtact ggccgcgccg
       61 gatcgatgcc ggcggtgagg tcctggcccc cgaccgcccg gagcgtgttg tgcagttcat
      121 cgacgtacgt gacctcgcgg catggatcat agagatggcc gccgagagga ggaccggaac
      181 cttcaacgcc accggccccg accacaagct agagatgggc caactgctcg aggcgtgcaa
      241 ggccgtcggc ggtgatgcgg agctcatctg ggtctccgag gatttcgtgg aggagcacgg
      301 agtggagccg ttcacggagc tgccgttgtg ggtgccgcgg aagaatgccg ccttgctgga
      361 catagattgc ggcagggcta tcgaagcggg actcaccttc cgttccttgt ccgagacgat
      421 cgaggacgtg ctcgagtggg accgcgcgag gccggtggat accgagtcgg cggcgggctt
      481 gacaccagag agggagcagg agttgctccg ggcctggcgc ggtgtcaccc tgtagaatgc
      541 ggtttgcgag atgagatgaa cctcacgcac gaatacatgc atcacaggac gggctacagg
      601 ctcggggccg ggtgctgctg gatcaggatc tacaaaggcg ccgagtgcga cgctcccgtg
      661 gtcgtgtgcg aggagctccc cagggtcgga gacgtcgcca ccagggaggt gtcagggtat
      721 ctggccgccg aggtggtcag ggagcacttc tccggtggtc tgccggatct accccgtccg
      781 atgctctgga tcgaacaccg cccggcccgc aggcgcgggc cgggcaagtt cttcctgcac
      841 accttcccct cctaccgtcc caggaccgtt ggcgcaggtt tcgtgcggcg cgtcaccctc
      901 ggaacgtcgc gtcgcgagcc gctggatcct gcagaggtgg ccgtcctcat tcgagaagtc
      961 taggaggcgg attgggcgaa gagaagggca cgaccgagta cgccataaag caggtcggag
     1021 accgttatta tcccgtcatc atcgacaaag atgcaggcgg tcactacgag ataaacaacc
     1081 ctctgaccgg cggcgtcctg agctacaaca accccgaagc cgcggagaaa tacatacagc
     1141 gcgcccgcga gaaagaaaga tcgtaggggc ggttcgcgct ccgtaacccg tacgttctcg
     1201 ggcaaggcgg cccctgccag cctcccgaag cacgaatgat cccgtcctgc gtgtagactg
     1261 ggtaacctaa agggaagcac gcggtgccgt cgcttggcac tccgaccgta ccgagaggag
     1321 accatggagc gtacagagag cagagtgggc ctgaagggcg tcctggcggc cgtcctgact
     1381 ccgatggaca aagacttgaa cccggaccat caagccttcg ccgcccactg ccagaggttg
     1441 ctcgcggcgg gttgccacgg gctttctgtc ttcggaacta cgggtgaggc aaactcccta
     1501 tccgtggacg agcggcttgc ggcgctcgaa gcgctggtcg aaagcggtgt cgccccggag
     1561 aaggtactgc cagggaccgg ttcctgcgcc ctcaccgaca ccgtacggct aagccaggcg
     1621 gcgctcgagg ccgggaccgc gggtgcgctc gtattaccgc cgttctacta caaaggcgta
     1681 ggggatgatg gtctgttccg cttcttcgcc gaagtcgtcg agcgcgtcgg cgacgacagt
     1741 ttgcgcctcc acctctatca catcccgcag atgacaggcg tcgacctcgg cctgcccctg
     1801 atctcacgcc tcatcgacgc ctaccccacc atagtagtgg gtacgaagga cagctccggc
     1861 gacagggaac gcatcatgac cctctgccgg gagtttccag acttctctgt ccttgccggc
     1921 acggagaccc tccttctaga aacgctgcgc agcggcggag aaggctgcat ctccgcaacc
     1981 gtcaacgtca catcccgcct cgcccgccgc gtacacgacg cgcacgcagc gggcaaagcc
     2041 gatgaggcgg aagctttgca agaacgcctc acgcacctgc gcgcatccat cgaagcatat
     2101 ccggtaatac cggccctcaa gcaactcatg acccgtctga ccgacgacga gcaatggcgc
     2161 aacatccgtc ccccgctctc cggcctcgac gaaaaacgca caaaagacct cctctccaac
     2221 gtgcccctag ccgaactgtt gtagatttac tgagagccga aggctgacag ctctcctatc
     2281 aggagaacaa ctcttcgaag ccaacttcgt cgagtatcgg aacgcccagt tcccttgccc
     2341 tctccagctt cgagcctgcg tcttcgccgg cgagcacgta gtctgtgttc ttgctgacgg
     2401 aggaggtgaa ggtgcccccg gccgcttcga gacggccgac gtagtagttg cggggacggt
     2461 ctagggttcc cgttatgacc acgcgcttgc ccgagagcgg accctccgcg gggccgccct
     2521 cttcgcgccc gaagtccagg ccccgctcca taaggcgtct tacgaggtcg cggttgtctt
     2581 cgaggtcgaa gtactccacg acggctcggg cgacgacctc tccgacgccg tcgatctccg
     2641 taagccgttc gacctcgacg cctcgcataa agtcctctcc accgaagcgc gtggcgataa
     2701 ggctggccgt aaccgaccct acgtgcctga tgccgagggc gtagaggacg agggagaacg
     2761 gcctttcctt gctcttctgg atggcctgga tcagattctc cgcgctcttc tctccgaagc
     2821 cttcgagggg gacgagctgt tcggccttca ggtcgtagac gttggcagcg tcgcttatga
     2881 agcctaggtc gaacagacgg gtggcgagct tctcgcccag accgtcgata tccatagcgg
     2941 acttcgagga ccagtggatg atgtgctcta gagcttgcgc ggggcaacgg gcgttcacgc
     3001 aacgggttac ggcctctcct tcggggcgcg agaccggctc gtcgcagaca ggacagttgg
     3061 cgggcatctc gaaggcgtac tcgtcgcctt tgcgttcctc tgtgacggct ctgacgacct
     3121 gcggtatcac gtccccagcc cgttcgacga cgaccgtgtc gccgacgagg atgcccttct
     3181 ctttgacgta gtcctcgttg tggagcgtcg cccgggagat aacgaccccg ccgacgttta
     3241 cgggctccag caccgcctgc ggcgtcagcg cgccggtcct gccgactttg acgatgatat
     3301 cgagcagcct cgtgcgccct gcgagcggct cgaatttgta ggcgatcgcc caccgcggcg
     3361 agcgggctac agagccgagg gcgagttgct gctcgcgcga gtcgaccttt accacgacgc
     3421 cgtcgatctg gtagccgatg gactcccgct ttctcgccca ccgttcgcac tcctcgatca
     3481 ccgtgtctat gtcctggtgg atctcgtagg ggttgacgcg caggccgtag gccttgagcg
     3541 cggcgagcat ctccgagtgg ctctcgaaga tctcaccccc ctccccgacg ccgtagagca
     3601 gtatggtgag gggccttcgt gccgtgatcc tggggtcgag ctgcctgatg gagccggcgg
     3661 ccaggttgcg cgggttggcg tacggtttct taccttcggc ttcgagctcc ctgttcaact
     3721 cctcgaagtc ggcgatgggg atgtacgctt caccgcgcgg ctcgagcacc ccgggcggat
     3781 cgtcgtcgag ccggtcgggg atagaacgta tcgtcctcag ctgcgccgtg acatcctctc
     3841 cgatgacgcc gttgccgcgc gtggcgcccc tgaccagccg ctcgttctcg tagcgcagcg
     3901 agacagccag gccatctatc ttcaactccg tcacgtagcg cggggtctcg tcctcgccga
     3961 gcaggcgccg tacgcgggcg tcccactccc tcaggtcttc tatcttgcgg gcgttcgcga
     4021 gggagagcat ggggacggaa tgcctgacct cctcgaaccc ctcgatgggc tcaccaccga
     4081 cgcgctgggt gggcgaatca ggggtgatga gctcggggtg gtcgtcctcc agggcttcca
     4141 gctccctgta gagggcgtcg tactcggcgt cggagatctc cggcgcgtca tctacgtggt
     4201 agcggcggtt gtggtaacgg acctgctcgc gcagatcctc taccctaccc cgaacgtcca
     4261 cgcttctggc cacgcaggaa ttatacgcat ccaggcaccc gctcccaagg gaagcctggt
     4321 tccacctctc ctttgtctag gtttgaccct cgtagtgttc gtggatggct tcgtcgaaag
     4381 ctcccgaaag gtctttcatt tgtggtgctg tctctatggt cctggcgttg tagggaacag
     4441 agacgcgttg gtcttcggcg tctacctcaa tcctctcggc cgggatgggg cgtcccccga
     4501 cgacgacgta tttcagcacg tcgctcgggg cgtcgtagac ggtgtcttcg atctccccga
     4561 cctcctcccc cgaggcgtcg tcgaccctgt agccctccaa ctgcgtgtag gggttatctg
     4621 ggcggtggtt cgctctctcc atacttcatc ctccggtggc aggcataaga gtatgcgcgt
     4681 atgatacttc ggcgtgggcg acagaacaga tcgaggccgg aacgcccgga gcctgaaggc
     4741 tttctcgatc ttgctccttg ccctcttggt ggcttcgctg ctgttcgtgc acacggcttc
     4801 atacgaacct gccgtctcct acatcctgct ctatttcgcc tcctccgtgg gcatcatcac
     4861 cgcggcgttc gtggttccgc tgctgccgga ggagcagttc accgccggtc tctgcggctg
     4921 gatctacgcc ctgtacgccg ccgtcgtcgc catgatggtc tttttctcgg gtgggacatc
     4981 gagcgagctg tacgtcatgt tcttcccgct gcttctggcc tcggcgctgc acgggtcgtg
     5041 gaggctggtg ctcgcggtcc tcgccgccgt ccttttctgc cacgccctgg cgatgttgcc
     5101 ggaccttctc gagagaggga cgggccagga gatcgcctcg ctcgtcttct acaggctcgc
     5161 cgctttggga ctgaccggcc tgttcttgat ctacgtagcg aagagcttgc tccctgcagg
     5221 tatcgacgag gactacgcga tggatgagga cgggtccata ctgctcgggc ggatcgagga
     5281 ggagctcgca acgcgcgggg aggcgccagt cgccgtggta ctcgtcgacc cgggagccga
     5341 gctggaggcc atggacctcc tcctggagag ggtgtggtcg cgcatcgggg aacccgtatt
     5401 gctgggcgag ggcaacctct tcggggtggt cgtaagcggt gtagacgatg gagaggtgga
     5461 gagtgcggcg cggcgcgtcc tggccgccgc gagctccctc ggcgcgcaag acactagggc
     5521 aggggcggcg atctacccgc gcgacgcccg gtccgcagaa gacctcctga tcgccgcagg
     5581 acgggcgctc gaagccgctt tcgaaggcga gtcacccggc gccatagtcc tggccggctc
     5641 cgccagcaaa tcagcacgct caggctagcg ccttcgcttg tcagcacggc gcttcgcgcc
     5701 ttgtcagcat gtcagcaagg gctggaacgg gagaagctga cctgctgaca agcgagggcc
     5761 ttataggccc gaagcgtgct gacaagttgc cgcaggcagc gtgctgaaac ccccgaaggg
     5821 ggttccacgt accgatgata taatccacga cgaactttgc aggcgtgacg actgccgggg
     5881 gtgggcggca cagatgggac gatttacgag ggctattcgc ggtttcttcg ggaggtttct
     5941 ctccggggtc gagcagcgca acccggagat actgctggag aacgccgtcc aggacgagct
     6001 cgaaaacctg aagaagctcc aggtcgctgc ggcccgcacg atggcctacg agaagatgct
     6061 cgccaacgac gccgttgcga agcagaagac cctcgccgtc aaggaacgtc aggcccgcga
     6121 gctcgccagc aggggacagc gggaagccgc gatcgaggtc gtccagcaga agcaggaggc
     6181 gcaggcgagc ctcgcggata tcgaatcgcg tctggaagat gcccgcaaga actcccagga
     6241 gctggagcag gcgttccgcg accaggagcg ccgctacaac aacgtggtgc gcgaacgtgc
     6301 ggccctgatg gaggagcacc agcgctcccg tgccctgagg accgcaaacg aagcccgctc
     6361 caacatctcc ctctccgact catcccgcga cctggagaag gcgcggcagt ccatccgcca
     6421 ggcctcctac gaggcggagg ccataggaga gctcggcacc agcgagaccg agcgccagat
     6481 agcggcggcc gagatagaca taaagcggct cgacgccgag cgggagctcg agatgatgga
     6541 ggtccagatg ggcctgcgcg agccggaacc gatcgggccc gcgccgaccg aggagacagc
     6601 gagcccccag gccgaagaga ccgaggaggg accgcgtgga caatagaaag aacctctcgc
     6661 ggcggctgat ggtccgcctg gcgcctttcg ctacgttcgc aggtctcggg accttcgcgg
     6721 ccttcggaga agcctacgtc ctcgccggcc tgctaggcat cggggccttt gtcacgggcc
     6781 gtaggctcct cgacaggcgc gagaacgcgc gcaaggaact cttgagcagg gtcaggcaca
     6841 acaggtggga gctcggccgg gtggcgaggg aggaccgcat cgcggcgtcg cagatgaagc
     6901 ggctgatcgc cctgcaagac gggctgctgg agagctggga gcttctgccc gaggagtgca
     6961 ggccgctact ggacgaggat atcttcacca tcgtaggcga gatcgagggc gcggcgcgtc
     7021 tggcgcgccg gagggcggcg ctgaggaggc atctggagag cgtggaccgg gattctatcc
     7081 acggtcgtat ccaggagttg cagaacgatc tcacgcgcct ggagccggaa tcgcccatgc
     7141 gggcgccgtt cgagagcgcg ctccaggggc gccgcgggga actcgcgggc taccacgatg
     7201 tgctcggcgg catcagcatg atcaacgccc agctcgagag cgccgagagc ttgctgggta
     7261 atctgcgtgg agaattgttg gccctcgacg acagcatcgt ctccggatct ggggaccctg
     7321 gcctcgcgcg gctcaaagag cgggtcgctc ttttccgcca cgggctcgat gaggtgacgc
     7381 ggcgcgtcga gagcctaccc gctacggaaa ggatatcgac tagatgaacg aggatcgcaa
     7441 cacggcccgg cagagcttct tctccagggt gttcacgggg aacacggagg ccgaggggac
     7501 ctccgggacc tccatctact cctcggagga gatcggcatg ccggggactg aagaggcgga
     7561 gggcctggcg gcgcacgggt tcaccgtcga gcgggccgcc gaggtcatca ggaacctccc
     7621 tccagaggtg ccgaggccgg ccgccgtgcg catcgtacgt gggacgctag aggcggccgg
     7681 tatcgacatc gccgagctgg agacctcgac cagggccagg gagtccaggc tcaactccga
     7741 gattgacttg agcgagaacc gtatacagca gttcaaggac gagaccgaag aggtgatccg
     7801 gaacctcgaa gaccagatac gcaaggcgcg cgaggccaga gacttcggcg tctccgaaga
     7861 ggagagaaag atctcctccg ccgaggccgg cctcgataac atagacctgg tgcgcgactt
     7921 cttcgggctg ccacaggaag agacggacga accgcccacc gccggtgaag agacgcaggt
     7981 catcgagggc gtggaagagg aggaggacga cacccaggtt ctccgccgtc caggcccgct
     8041 ctcggactct gaggactacc tggaacccgg ggatgaaaag gaacagtgag catcccgcgc
     8101 cgcctggggc gcctcgccca gggtttcgtc tccagcctgc gggaagatga gcgattccgc
     8161 gaaaggctcc gtacaggacg cgagcatagc gaggccttga gaggagcctt cgaagcggcc
     8221 tggagaggcg cctcagagga gtggcgcaag gcggaggaac gccgcgcagc acaggaggcg
     8281 accgcggaac agacctgggg atggcggtcc gcgaccacca cgttcactcc caggaaatac
     8341 ccgcccaacg tcctggccgc ctacgacagg ctcggcctcg tccccagcgc ttccatggaa
     8401 gaagtcgaca ggaaacgcag agagctggtc aagcgcttcc acccggatcg tttctccgat
     8461 ccggagaagc gcctccgcgc cgaacgcgta acggcagaga tcaacgccgc ccacgacgcc
     8521 atagaacgct atgaagcggg gtgagggagg gttaaccctc cctcaccccg cgcggttagc
     8581 attccgctat cagccttctt tgctgacggc tgacatacac gtggctcgcc gtctccgagc
     8641 actccataac ctctcgttca cttcaggggc ctgcagagaa cctcctcagg aggaagttcg
     8701 cacaatgcta cttctacgca attggatgat agggagaata gaccgtgcaa ggggtagaat
     8761 actttcgtgg tcgaaagaag aagacagggc tcgggttggc agatggcaga gtcgcaactc
     8821 tcggagttgc ggaatattcg cgtgctactt gaagaggcga ggggattgtc cagaagtctc
     8881 gcctactacc gtagggcacg gttggaggag ggagtaggcg cggtcctcga tgaggtggat
     8941 cgccagattg aagagctgcg ggccgaccgg cgcggctaga gccatgccga tccatcgcag
     9001 attaccccgg tcctctgtcg gcatggcgca tctcctatgc gccagatgcc tgcagcaggt
     9061 gaagctcggc acgtttgttt ggcacacccc tcccggccca tccccgtggg ctgacggagg
     9121 ctacgacgcg gtcgaacctg cgatcatcgg tagccgtgat cagcgcgtac aaacgccgaa
     9181 tacctccttg cgggcaagct cgataacagg tcactcatcg ccgagccaac ccgcgtatgt
     9241 gccagcggat ctcgacagac cagcgtactc ggtgaggacg aaaaatggct tgaggtattt
     9301 atgaccttta cgtgcgaaga ggcgtgccag cagggtttca gcgtgctcgc agttgtgcgt
     9361 ccgaatttgg ttgaaagcac cttttacgaa ggtctggaga ataggtccgc ggccctgctt
     9421 gagaactgcc ctgtggcctc agatgtttga accggagcgc aagaaagacc ggagcctcgc
     9481 ggtcgcacct tggccctttc tctgcgcgct tccaacgcct gcgcagtaca gtgccttcct
     9541 accttagcgc aacttcgcgt tgccgagcag cctgcgtatc gtggggcgca cttgccttgc
     9601 attgtcccca gaagttatgg ttgtgtcctg tcgtttttgc ccgctaacga aggagaagct
     9661 cgatgcgcgg acgcaggcgc cgtcgcaggc gaggacgcaa gggtcgcaaa ggtagccttg
     9721 gcacccgccc cgagaaatcc atagacttga tggtagaacg cagccctaag agcaaggtaa
     9781 agtctgcgaa ggtgacgaga atacgccgcc ggaatggaga ggtcaccgaa gaaaggtacg
     9841 aactctaagg ggtataccta ctcgcagagt aggtatactt gccgcagagc atgttcagtc
     9901 tctgatctac gcctgactct gacttagtag gctcaactag atacgaaaga ataccgccca
     9961 gcataggacc gggatcctcc agacaggcat taccgcggag gggtgtcttg tttgcgcgga
    10021 tacgatcggt ttgtacggtc ttctccatgc ataagaatct ctcggccacg acgaagaccg
    10081 aggccccgaa agaaggaccc cggcccggtg agtggagcgc ttggcacgtc gctggctgcc
    10141 ttcccctcct atggcgtcta gaggccggtg agattcccta cggggccgcc cgcgctgttg
    10201 agccgctcgc attcttcgca aagggtcttg ccgcccgtcc ccttttggac gttctcgggg
    10261 gcgatgctgt tgccggtctg gcagttgggg ttgttgtggt agacgtcgga gttggccgag
    10321 tgccaaggtc ccccaccccc caagccggca ctcgtcgtct ccctcacgct atcggcggcc
    10381 ccgcccacaa tatctgcggc tcctcccacg agccctttgt ccttgtccgt agccacgcgt
    10441 ttcctccttc gttagcctac cggtcgttca gtatgttact agatcgcgcc gagtctagcc
    10501 tacgggggct ctcccgctgg ccccgcgccc cgagagctcg ccttctctcc gagggacacg
    10561 agccatgtcc ctcttcgcca cgagatgctc tagcggccgg tcagcttgtc cgtgatgcct
    10621 cccacgtgct ccgtcgcgtc ggtgatgccg cccaaggggc ccttgtcctt gagcctttgc
    10681 ctgtctcgct ctttctccgc ctgcttggct tcggcctcgg cctgcctcgt cttcttcctc
    10741 tgctcggctt ctgcttcggc cgcggctctt ctctgctcgg cgcggccctc ggcctccttg
    10801 gccttgccca tcgccttgtc cattgcccta cctccccatg gctcgtctgc gctcttcaat
    10861 tggtgtcttc gtaacgttag agacacgaga gggccgatgc ggttacgccc aagacgttcg
    10921 taagggcgca caagacaacc tgcttttgcg tgcctttaca ggcgtacacc gaagaacata
    10981 ccttctaggg cccgcggtgg agaagaacga cccaccctcg accttctcgg tggtctacct
    11041 caggttctct tgaggaggtg ttcaccgagt ccgaggttcc gttcggccag cgaaaagtac
    11101 agcatcgtca gaactcccac ctgtttgccc agtggggagg ggggttatgg ctatggcgtt
    11161 cggcatcatc tcgatcaggt tccggtcgca ccgctcgtcg aaatcaccca agaggctgta
    11221 gcaccgatcg tgctcccggc agacgagatc cacggcgtct atgggcggcg tctaccaggt
    11281 cggatcgccg tggccgaatc cacagtaatt gccgtagaga ggaaggctcg gccgctgagg
    11341 tgcatccggg cgttcgggct gactgttcct ggccacgcgc acttcccctt tctaagtacg
    11401 acaaggtctc gccactttac tcatcctttt cgagcaagac tcctcctaaa cccccgagac
    11461 ggagaaataa tcgagacgaa gttcgcagag cgctcttttc caagcactcc agcgaataag
    11521 ggtaagtagg atactcgggg ctgacatagc tgtggtaccc ctctgccggg cataaccttg
    11581 ggctctcttg ttctcgcctt cgatgatggc gccgactgct tccttcgtcc tgcctttaca
    11641 tatcctcgac gtttgcgtgg cgactttggt cgttcacttc cgggcaagta aagatcggtg
    11701 tggctgcctt tccgtcgtag gtagtctcga cgattaagtc ggccgcaccg acgctgcgca
    11761 ataaacacta tgagccgcct ccgtcggtgg cgtccttggc ccgagttgcg agtctctcgc
    11821 ttggcctctt tgacgacccg ctttccctac ctctcccttg cgcaacctcc ataaagatct
    11881 gaggcgctac gtcggccatc tctgagttct atgagcgaac agctccccta gccgaagccc
    11941 gggcttgccc ggctctgtat ggagcttttc ggcaagctcg aaagtgatgc cacccacctc
    12001 gaagtacttc caggagaccc agccttcacc tacgggagaa agcgagccgg ttttcactaa
    12061 gatcgcgcaa cccgagaaaa tccaagagag ttagctaccc actggaggct ccggaacact
    12121 tactaaagag gcttaccaca aggtcttccg gcactcaact cggctggacc atgcggacct
    12181 tggccaccgc gcggtccagc tcaaacgcct gttggagata caaagatgag ggcgtaggag
    12241 tgccaaactc ctttggcaga gactctagct caccagttcg gcgcgtacta tgaggcggtt
    12301 ctcgaacgtc ggtatagggg tacagctctg aagcgagact acggtcttgc cctctacagg
    12361 gtaggtcgcc cagtagtcgt ttggctcgac ggtcatgagg tcgtagaccc tgaacgtgta
    12421 ttcttggcct tccttgtctt cgacgaagat ctcatcgcct ggtttcatct tgtcgaggtt
    12481 gtagaagacg tagggcaccc tggtccgcgg gaaaccgagg gcgtgccccg tgataaacgt
    12541 gttcgagccc gctatccagg gcaggccgct gccacgcacg cggagtatgc cctccctgtc
    12601 gagctcggc
//
LOCUS       DATMVF010000063        16840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_235469, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000063 DATMVF010000000
VERSION     DATMVF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16840
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235469"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VN178_02815"
     CDS             <1..405
                     /locus_tag="VN178_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79931.1"
                     /translation="IWSLYMQSAVQKYPVREFDTPSPDLPLEVKTDGRAYVKPEPPPK
                     PEEPKEKLSTNAKEEDPGPENTTTRPEEPGPSEEQPPSAPDPVQERTSQQFPASASPS
                     SASPEPDGNVRGDSQPRVFATPQERRGYREPE"
     gene            409..1569
                     /locus_tag="VN178_02820"
     CDS             409..1569
                     /locus_tag="VN178_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS79932.1"
                     /translation="MKQAGFARDAATWMTYGLVGFFAFMETVLGPIMPFLRRELDLGY
                     AAASLHFSAFALGAVVLGFFGDRLVGRWGRRAALWGGAFGVAAGAILLISSPSVWGTV
                     PATFTMGICGALVLITSQAVLSDRHGEYSAVAVTESNVTASACAIVAPLLVGASAASG
                     LGWRAALILPVVALAMIAARFFSRTLDLPPNAAKDDAPADGPTLSPLYWAYWTLVALG
                     VASEWCIGYWGADFLADGTGLTRPAAATSLTAFFAAMLAGRVASSRLARTMPPAVLLA
                     MTLFVAFLGFPLFWLSPGTLLALAGLFLTGLGIGGVYPLGVSAAIAAAPGNTDAAAAR
                     LAIGGGGAILVAPFVLGALADRVGIGTAFGIVVPMLLAAWSLALIAGRRGRQ"
     gene            complement(1611..2408)
                     /locus_tag="VN178_02825"
     CDS             complement(1611..2408)
                     /locus_tag="VN178_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HWS79933.1"
                     /translation="MGDALDTSEELHAFVCDVARRAGELQTSRYHDPGEVREKAPKDF
                     VTEVDLLCEELMVNAIGERYPDDAILSEERGGSVSEAGRTWLLDPVDGTANYMKANPI
                     FCACVSVIEGGTVTHAAVAAPRLGDLYHARSGGGAYKESGGRETPLRVSDTRRLEYAV
                     AGADLSLHGGKPQDPGVERVLCRAWQLRALGSAAIRGAWLAAGYLDVSVGTYNTAWDY
                     APTVLLVSEAGGRATDLSGEPWSLSSDGLIATNAALHDEVLQTLHSG"
     gene            complement(2425..3528)
                     /locus_tag="VN178_02830"
     CDS             complement(2425..3528)
                     /locus_tag="VN178_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS79934.1"
                     /translation="MATQNSMMLAERTDELLNRGRSQGFLSTEEVANLLQEGDLSAAE
                     VEEFYATLEEEEITIVEGEASENGGSRTTVTGVVHVSEPPAVQIAHAVATGDSIRMYL
                     AEIGRVPLLTHADEIRLAKGIARGCKRSKDRLVEANLRLVVSIAKKYRNRGVSFLDLI
                     QEGNLGLIRAAEKFDHSKGYKFSTYATWWIRQAITRAIADKGRTIRIPVHMVEKVNKY
                     HRTHRRMIQTLGREPSEEEIALELGVFVAEIQRLQEISQRSISLATPVGDDDSSELGD
                     FLEDAGSVTPTDAVSESLLKAHLREALDELPERERQIIELRFGMKDDRPRTLEEVGRE
                     FDITRERVRQIQMKTLNLLREQRRTQNLREYLN"
     gene            3821..5275
                     /locus_tag="VN178_02835"
     CDS             3821..5275
                     /locus_tag="VN178_02835"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWS79935.1"
                     /translation="MILDRAKLTGDLGTEIPSGVWGVRGVTHDSRCVEPGFAFVAIPG
                     FKRDGTEFAPEAVRRGASLIVAEHDIPGVPTAVVEDARGALAMLARRVNGDPSRGMEV
                     YGVTGTNGKTTTSYVLHSVLAGAYGEGECGLMTTAETISGGQSRPAVRTTPEATEVQG
                     TLASMLGSGVRRVVMEVSSHGVSLKRIAGTRFSGAIFTNMTRDHLDLHGSMGAYYAAK
                     RELFYMTEGPRLANAEDAYGRRLAGELENVSTFGLTAEADYRIAEVEPTRSGTGLTLH
                     HPEGVLRLETPLLGPYNVLNVAGAVSLALAIGTDETAVRRAVRGMGQVPGRFERVAAS
                     GEYGFEVVVDYAHTDVGLDAVLRVARGVAGSRGRVICVFGAAGDRDGAKRPKMGRVAS
                     LLADMSLITTDDAYSEDPEKIASQIEAGSDLSRTSIELDRRKAIRRALEMARAGDVVV
                     VAGKGHERVQHLPEGDIPFHDATVVRELLAEIGD"
     gene            5294..5554
                     /locus_tag="VN178_02840"
     CDS             5294..5554
                     /locus_tag="VN178_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWS79936.1"
                     /translation="MQRQTKSEVLRRLKSVEGHVRGVEKMVEEGDVTYADIVQQTNAI
                     FSAIRRINTVLLKDFVEERAERDGASDEMINDLIKAIDRVTK"
     gene            5668..5985
                     /locus_tag="VN178_02845"
     CDS             5668..5985
                     /locus_tag="VN178_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79937.1"
                     /translation="MVYSPGPMEDRGQQDDLGGLEAKVDELEKLTDSLDDVPEEELVG
                     ALGEAVELLAEINARIEARLESAGDESREIGALLGQVDFGPFDEAMEDHEHRERTTGE
                     PGV"
     gene            5969..6502
                     /gene="pyrE"
                     /locus_tag="VN178_02850"
     CDS             5969..6502
                     /gene="pyrE"
                     /locus_tag="VN178_02850"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWS79938.1"
                     /translation="MNRVSDLAPRIRDAALLEGDFVLSSGERSSFYVDKYLFSTEPNL
                     LRDVAGALAAEIPGGVERLAGVELGAVPLVVATALATGLPYVIVRKSAKEHGSSAGRS
                     IEGNLYQGERVALVEDVVTTGTQALRAARNLEETGVEVVTIVAVLDRREEDGGKLGGF
                     PFRFLLRMDDLRVVKPN"
     gene            6585..6857
                     /locus_tag="VN178_02855"
     CDS             6585..6857
                     /locus_tag="VN178_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWS79939.1"
                     /translation="MNKTALIEEIAGKANASKSDAQKFFDAFTEVVTDSLKNGEQVQI
                     TGFGKFYVQKRDARQGINPQTKQRINIPASKVPKFTAGNALKDQIK"
     gene            6940..7779
                     /gene="pyrF"
                     /locus_tag="VN178_02860"
     CDS             6940..7779
                     /gene="pyrF"
                     /locus_tag="VN178_02860"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWS79940.1"
                     /translation="MHALVDAARRKRSVLVAGLDPIPSRFPEELRVLPEAEATLKFCR
                     GILEAVEPYVAAVKLQAAFFERLGPAGMGIYADLIEAAAEVGLPTIADVKRGDIGPVA
                     TAYAGAHLGLYSATCVTVNPYMGEDAVLPFLEESRRQDLGGGVFVLVATSNPSSRKVQ
                     ETTAPPLFEVVAGLVAGLGSPAGDYPDAGAVVGVTRPEIGSRVRELLPETLFLSPGYG
                     AQGGDASGVRPLLDRRGGGVLVNSSRSILYAYESSATGYKEAAREAARKARDELHLAG
                     AHL"
     gene            complement(8102..8662)
                     /locus_tag="VN178_02865"
     CDS             complement(8102..8662)
                     /locus_tag="VN178_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWS79941.1"
                     /translation="MKRLAIVVFVVVVAALFYRSFGSAETGTGSLTLPQPAPSVGDSA
                     PAFRVKSVDDRVFELSDKGVYVLTFWSTLNKDSNEAVSGFEDLAREYEDDEASFAVVY
                     VNSAPRDDNVPYTMLLDSTGELVSKYNVKRVPRLFVVEDGTIAYAQDYYYEGYENGLR
                     EKMDEILASEPEKGHQSALREQEPDP"
     gene            8829..9788
                     /locus_tag="VN178_02870"
     CDS             8829..9788
                     /locus_tag="VN178_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HWS79942.1"
                     /translation="MILTVTLNAAVARTLVVPSLTLGHRHRAPESMTLAGGKGINVAR
                     SLRALDVPVLAAGFAGGRNGDAIRDGLSEAAIPFDLVEIEGYSRTSTAIIDPMAGTQT
                     EINEYGPEVTPAEAREFSRRLEYLMEYATAVVFAGSVPPNLDESFLVGLVRRARERGI
                     YTVVDSPPSVLRSALKANPSLVSPNQHEAESVVGYDFIEEGDFPLGLTRLVEHGAANA
                     CITSPEGLSYLSTENGTVSARAPRIEAVSTVGSGDAYLAGLLAGLLHRNLAPAEAVRL
                     AAGCAAANAETLGAGLFEARRAEELADSVQVGISSVSLQESRT"
     gene            9876..10187
                     /gene="trxA"
                     /locus_tag="VN178_02875"
     CDS             9876..10187
                     /gene="trxA"
                     /locus_tag="VN178_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWS79943.1"
                     /translation="MAVAEVTDATFESDVLRSERPVIVDFWAPWCVPCRRVSPILEEM
                     SESREDVAFVKVNVDDNPQAAMNYSISSIPTIARFEEGQVTQQAVGALPKNQLSAQLG
                     L"
     gene            10309..10578
                     /gene="groES"
                     /locus_tag="VN178_02880"
     CDS             10309..10578
                     /gene="groES"
                     /locus_tag="VN178_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWS79944.1"
                     /translation="MKFKPLGERALVQIVEREETTASGIVLPDTAKEKPQTAEVVAVG
                     QFENGVKVTEGDVIVFAKYSGTEISLDGEDYMILDADDILGIVES"
     gene            10689..11759
                     /locus_tag="VN178_02885"
     CDS             10689..11759
                     /locus_tag="VN178_02885"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWS79945.1"
                     /translation="MRTLRRGDRGREVVDLQTRLQALGFDLGNRGIDGVFRERTELAV
                     RAFQQHLGLLADGLVGPITWREIVEAGYRPGGRLLYLRQPPFRGADVVELQQMLDDLG
                     FDPGAVNGLFDPRTVRAVRDFQRNAGLQDDGVVDTAVFKVLNTFAAQSLGTHQLPDKS
                     GGYFTEDLFSGAIVVDAAHGGRDTGYESPTGISEADLNLAVAQELAQILPAREVLLTR
                     SGDEEVSAVDRAFLANSSKARMIVSIHHASHPTPTASGTASYYFGRLGYQSHRGRMAA
                     AYLQRGISQALGTRDIGEFGRSFEILRETNIPAVILEPLYLTNPGELDLASDPYYPAT
                     VAEAIASALELYAGRDKAFIAV"
     gene            11965..12038
                     /locus_tag="VN178_02890"
     tRNA            11965..12038
                     /locus_tag="VN178_02890"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11999..12001,aa:Pro,seq:cgg)
     gene            complement(12294..14186)
                     /locus_tag="VN178_02895"
     CDS             complement(12294..14186)
                     /locus_tag="VN178_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWS79946.1"
                     /translation="MKVIVASDLVKYHGGDLKVLSGATLSVEAGEKIGLVGRNGAGKT
                     TLLDLLAGHSEPDSGSVEHPGGAKVGMTAQKLYLGERGVLTLEEEIISAFEPLMAREK
                     ELEELGTRLSEDPSNTLIERYGRLQSEFERDGGYEYRARAASTITSLGFSPEDWKRPV
                     GSFSGGEQSRIALARLLMEEPDLILLDEPTNHLDLRAIEWLEAFVKGARSAVLVVSHD
                     RYFLDAVAGSILDLEDGRLTRYPGNYSKYVAEKRSREEQLARKAKANAERRAQLERFV
                     EKNRAKASKASQAKSKQKLLDRMEQIEDPRRSSKNVKVYLGGETVRAGRVVLEMEDVR
                     YAHDDAEEPLLEGLDLVIERGERVALLGPNGTGKSTIMRLATGELSPQRGTVRPGNNV
                     TLAYQDQQLARLDDSKTVLGEAMEATGFDAPEARDLLGAFLFSGEDVFKKVRSLSGGE
                     RNRLSLAEIVVSGANMLLLDEPTNNLDIPAREALEDALLEYRGTMFFISHDRYFLRKL
                     ATRVVDLEGRRLRNYLGGYDYYRSHRRLDEGSKGTKGRQRRRIRPGQSKDQSVLATRL
                     VAVEGEIDATERRVARLEKELATSELYSDGKRSREIVTEHRELKGALDRLYADWEALL
                     EETEQS"
     gene            14185..15009
                     /locus_tag="VN178_02900"
     CDS             14185..15009
                     /locus_tag="VN178_02900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS79947.1"
                     /translation="MAQRLYKGDHLTPEEREELEDDLIAFCDRELDLLGDISGLDVLY
                     AGGSSPLWIEGLSQRVGENGSVTAFDMDAVRVDDTRASLGEAGLAAPVCLLAADLFGM
                     PFDPSTFDLVYSSGLFHELDVKGRSAQEALAALHVMARSGGKVATSDFVDSEPAVQLE
                     EEKLQAESRREAYGIELYGIGPPERLVALHEKHLENVRWLVSPPRRIHHLGKLVLAEE
                     EPAELSLLPPATARRLRESRDALRGRILGEGYTRPATLYVEGLVRESRDLEDHPIE"
     gene            15083..16726
                     /locus_tag="VN178_02905"
     CDS             15083..16726
                     /locus_tag="VN178_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWS79948.1"
                     /translation="MTIIGLSSVEKFYGGRAVLRGLGMRVNAGARIGLVGGNGAGKST
                     VLRILAGAEDVDGGEVIRRRGSSVATLPQHIEGDERTPMEVVRAARPEISDLQEELQA
                     CEEQLGSPGVASDLRRMQRVLERQDRLLRRFTELGGPGFVGEARGYLRSLGLGDDDIY
                     RPMSDLSGGQRKLAVLAACLARRPDVLLLDEPESHLDAGRRERLEAIVRTFDGAVVIV
                     SHDRYLLDETVEEIAELEDGRITSWPGNYSAYTVARELKLKRQQQLYVAQQKEIARLE
                     EAIRRFKLWASLVVNERHIKQARNKQRQIDRMDKVERPVLERRKIGLEFRGRVRGGRK
                     VIELQDVSVAFDDEPVLIGVDLTVSRGERLGIVGPNGAGKSVLAKLLADILPPTEGER
                     WAGPSIDIGYLAQNDEPPPGASPLGLVRDAKPIYEGDAVKLLGRFLFRYEQVREPVTS
                     LSGGERTRLQLLLLMLREPNCLVLDEPTNHLDIDSLEILEDELERFPGTVIFVSHDRY
                     FLDRIADGIVEISEGEVHRHTGGYSDWRMKASQPAAFGS"
BASE COUNT         3033 a         5277 c         5494 g         3036 t
ORIGIN      
        1 atctggtccc tctatatgca gagcgccgtc cagaagtacc cggttcggga gttcgacact
       61 cctagccccg acctgcccct cgaagtgaag accgacggcc gcgcctacgt caagcccgag
      121 cccccgccaa agccggaaga gcccaaagaa aagctgagca caaacgcgaa ggaagaagac
      181 ccaggcccgg agaatacgac taccagaccc gaagagccgg gtccctccga ggagcagcct
      241 ccgtccgcgc cggacccggt tcaggagcgg acatcgcaac agttcccggc gagcgcttct
      301 ccatcatcgg cgtctcccga accggacggg aacgtacgcg gggacagcca gcctcgagtc
      361 ttcgccacgc cccaggagcg gcgtgggtac agagaaccgg agtagttcgt gaagcaggcc
      421 ggcttcgcga gggacgctgc cacctggatg acttatggcc tggtcggctt cttcgcgttt
      481 atggagacgg tgttggggcc gatcatgccc ttcctgagga gggagctgga tctcggctac
      541 gccgcggcga gccttcattt cagcgccttc gccctcggcg ccgtcgtgct cggcttcttc
      601 ggagacagac tcgtgggccg ctgggggaga cgcgcagccc tctggggcgg ggcattcggc
      661 gtggccgccg gcgccatact cttgatctcg agcccgagcg tctggggcac cgtacccgcg
      721 accttcacca tgggcatctg cggcgccctg gtactcataa cctcccaggc tgtactctcc
      781 gacaggcacg gtgagtacag cgccgtggcc gtcaccgagt ccaatgtcac ggcgagcgca
      841 tgcgcgatcg tcgccccgct cctggtcggc gcgtccgcgg cgtcggggct tggctggcgt
      901 gcggcgctga tcttgcccgt ggtggccctc gcgatgatcg ccgcccgctt cttctcccgg
      961 accctggatc ttccgcctaa cgcggcgaag gatgatgcgc cggccgatgg accgaccctg
     1021 tctccgctct actgggcgta ctggacgctc gtggcgcttg gcgtcgcctc ggagtggtgc
     1081 atcgggtact ggggcgcgga tttcctcgcg gacgggaccg gcctcacccg cccggcagcg
     1141 gcaacctccc tgaccgcctt cttcgccgcg atgctcgccg gccgcgtcgc gagcagccgc
     1201 ctggcgcgca cgatgccccc agcggtcctc ctggccatga ccctcttcgt ggcgttccta
     1261 ggtttcccgc tgttctggct ctctccgggg actctcctgg cccttgccgg tctcttcctg
     1321 acggggctcg gcatcggtgg cgtctacccg ctcggcgtct cggcggccat cgcggcggcg
     1381 ccgggcaaca ccgatgcggc cgcggcgagg ctcgccatcg gaggaggcgg cgccatactc
     1441 gtcgccccct tcgtcctggg agccctggcc gacagggtcg gcataggcac cgccttcggc
     1501 atcgtagtcc ccatgctcct cgctgcctgg tctctggcac tcatcgcggg acggagagga
     1561 cgccaataat aggccaacgt ttcgacgcag ttacgtagag tcgagcgtgg tcagccgctg
     1621 tgcagtgtct gcaggacttc gtcgtgcagg gcggcgttgg tggcgatgag accatctgag
     1681 gagaggctcc acggctcgcc cgagaggtcc gtggcgcgtc cccccgcctc ggagacgagg
     1741 aggaccgtgg gggcgtaatc ccaggccgtg ttgtacgtgc cgacggagac atccaggtac
     1801 ccggccgcga gccaggcccc cctgatcgcc gcgctgccca gagcccttag ttgccaggcc
     1861 ctgcaaagca ctctctccac ccctgggtct tgtggctttc cgccgtgcag ggagaggtcg
     1921 gcgccggcga cggcgtactc cagcctcctg gtgtcgctga cgcgcagggg tgtctctctg
     1981 ccaccactct ccttgtaggc gccgccccct gacctcgcgt ggtaaaggtc gccgagtcgg
     2041 ggcgcggcga cggcggcgtg ggtaacagtg ccaccttcga taacagagac gcaggcgcag
     2101 aaaatggggt ttgccttcat gtagttcgcc gtaccgtcca cggggtccag gagccaggtg
     2161 cgcccggctt cggagaccga cccgccccgc tcctcggaga gtatggcgtc gtcggggtag
     2221 cgctccccta tggcgttgac catcagctct tcgcaaagga ggtccacctc ggtcacgaag
     2281 tccttggggg ccttctccct cacctcgcca gggtcatggt agcgagaggt ctgaagctca
     2341 cccgccctgc gggccacgtc acacacgaac gcgtggagct cttccgaagt gtccagggcg
     2401 tccccgatct tgggtagcgg ttctctagtt gaggtactcg cgcaggttct gggtgcggcg
     2461 ttgctcgcgg agcaagttca gcgttttcat ctggatctgg cgtacccgct cgcgcgtgat
     2521 gtcgaactcg cggcccactt cttcgagggt gcgaggacgg tcgtctttca tcccgaagcg
     2581 gagctcgatg atctggcgct ccctctcggg cagctcgtcg agtgcttctc tgagatgggc
     2641 cttgagcagc gattcggaga cggcgtccgt gggggtcacg gagccggcgt cctccaggaa
     2701 gtcgccgagc tcggaggagt catcgtcccc gacgggcgtg gcgaggctta tggaacgctg
     2761 gctgatctcc tgcagcctct ggatctccgc cacgaaaacg cccagctcga gcgcgatctc
     2821 ttcttcggaa ggctcgcgtc ctagggtctg gatcatgcgc ctgtgggtcc tgtggtactt
     2881 gttgaccttc tcgaccatgt gaacggggat gcggatggtc cgccccttgt cggcgatggc
     2941 gcgggttatc gcctgcctga tccaccacgt cgcatacgtc gagaacttgt agcccttgga
     3001 gtggtcgaac ttctcggccg cccttatcag cccgaggttg ccctcctgga tcaaatccag
     3061 aaacgagaca ccccggttgc ggtacttctt ggcgatggag acgaccagcc tcaggttggc
     3121 ctcgacgaga cggtccttgg acctcttgca tcccctggcg atgcccttgg cgagccttat
     3181 ctcgtcagcg tgggtaagaa ggggcaccct tcctatctcc gcgaggtaca tcctgatgga
     3241 atctcccgtg gcaacggcgt gggcgatctg gaccgcagga ggctcggaga cgtggacaac
     3301 accggtcacc gtcgtcctgc tccccccgtt ctcggacgcc tcaccctcca cgatcgttat
     3361 ctcttcttcc tccagcgtag cgtagaactc ctctacctcc gccgccgaga gatccccctc
     3421 ctgtaagaga ttggcgacct cctctgtgct gagaaagccc tgacttcgcc cccgattcag
     3481 aagctcgtca gtcctctccg ccagcatcat ggagttctgc gtcgccattt atctcctctc
     3541 ctgtgcctct gtgtaccaac cgacggcagc cggatacaca tcgacgccat tgtgaaatcc
     3601 ctgtaaaaaa tgacccgcgt agggtagcac accacatatg gtgagtcacc gtatttttcg
     3661 acctatttcg tgtgtgttct tttaccacat ttccgctttc ggaaaaccgg gactggtttc
     3721 agcgctgttt cagcggtatt tcgggcatgt ttcggagtgt tgtgttctct tgagaaaatc
     3781 tataggaggt tctcgttacg gctcttgtta aactaggacc atgatcctgg accgggcaaa
     3841 gcttaccggg gatctcggga cggagatccc ttccggcgta tggggcgtga ggggggtgac
     3901 tcatgattcc cgttgcgtag agcctggctt cgccttcgtc gcgattcctg gcttcaagcg
     3961 tgacgggacg gagttcgcgc ccgaggccgt ccgtcgcggg gcctccctca tcgtggccga
     4021 gcacgacatt cccggcgtcc cgaccgcggt cgtggaggac gcgcggggtg ctctggcgat
     4081 gttggccagg agggtcaatg gggatccttc gcggggcatg gaggtctacg gcgttacggg
     4141 caccaacggc aagaccacga cctcctacgt cttgcattcc gtcctcgcgg gagcctacgg
     4201 tgagggcgag tgcgggttga tgaccaccgc cgagacgatc tctgggggcc agagccgtcc
     4261 cgcggtacgg actacgcccg aggccacaga ggtgcagggg acgcttgcct cgatgctagg
     4321 gtcgggtgtc cgcagggtcg ttatggaagt ctcctcgcac ggcgtgtccc tgaagcgtat
     4381 cgccgggacg cgcttctcgg gcgcgatctt caccaacatg accagggatc acctggatct
     4441 ccacggctcg atgggggcct actacgcagc caagcgggaa ctgttctaca tgacggaagg
     4501 tcccaggctc gccaacgccg aggacgccta cgggaggagg ctggccggcg agctggaaaa
     4561 cgtgagtacg ttcgggctca cggctgaggc tgactacagg atcgccgaag tcgagccgac
     4621 ccggagcggg accggcctca cgctacatca tcccgagggc gtcctcaggc tcgagacgcc
     4681 cttgctcgga ccctacaacg tgctcaacgt cgctggggcc gtctccctcg cgctcgcaat
     4741 cgggacggat gaaacggcgg tgaggcgcgc ggtgcgaggg atgggacagg tgcccggacg
     4801 cttcgagagg gtcgcggcgt ccggagagta cgggttcgag gttgtcgtag actacgccca
     4861 caccgacgtt gggctggatg ccgtcttgcg ggtggcgagg ggggtagccg gtagcagggg
     4921 acgggttatc tgcgtgttcg gggcggccgg agatagggat ggggcgaagc ggcccaagat
     4981 gggacgcgta gcctcgcttc tcgccgacat gagcctgatc acgacggacg acgcctactc
     5041 ggaggatccc gagaagatag caagtcagat cgaggccggc tccgatctct cgcggaccag
     5101 catcgagctc gacaggcgga aggcgatccg gcgcgcgctg gagatggcga gagccggtga
     5161 cgtcgtagtg gtggccggca agggacacga gagggtccag cacctccctg agggggacat
     5221 ccccttccac gacgccacgg tggtcaggga gctactggcg gagatagggg attagaggga
     5281 ggtagcgagg gcgttgcaga ggcagaccaa gagcgaggtg ctacgccggc tcaagagcgt
     5341 ggagggccat gtcagggggg tcgagaagat ggtcgaggag ggtgacgtaa cctacgccga
     5401 catcgtccag cagaccaacg ccatcttctc ggccatcagg cgaataaaca cggtcctctt
     5461 gaaggacttc gtcgaggagc gggccgagcg ggatggcgcc tccgatgaga tgataaacga
     5521 cctgataaag gccatagacc gggtaacgaa gtgaaggtaa ggtgcgggct gtggtgtcct
     5581 tgaccggggt gcctttcgcg cctcgctcgt cgatggttgc ggcgctcgac gggcgcgggt
     5641 cttccggggg ccgaagacgg ggttgggatg gtgtatagtc cgggaccaat ggaggatcga
     5701 gggcagcagg acgatctggg aggtctggag gcgaaggtcg acgagctcga gaagctcacc
     5761 gactctctcg atgacgtacc agaagaagag ctggtagggg ccctgggcga ggccgtggag
     5821 ctattggccg agatcaacgc acgtatagaa gccaggctcg agagtgcggg ggatgagtca
     5881 cgcgagatcg gcgcgctcct cgggcaggta gacttcgggc ctttcgatga ggccatggag
     5941 gatcacgaac acagggagag gaccaccggt gaaccgggtg tctgatctcg caccccgcat
     6001 cagggacgcg gcgctcctgg aaggggactt cgtgctctcc agcggggagc gcagcagctt
     6061 ctatgtggac aagtacctct tctcgaccga accgaacctc ctgcgcgacg tggccggcgc
     6121 cctggcggcg gagatacccg ggggagtaga gcggctcgcc ggtgtggagc tcggtgccgt
     6181 tcccctcgtt gtcgccacgg ccctcgccac agggttgccc tacgtaatcg tccgcaagag
     6241 tgccaaggag catggcagct ccgccggacg cagtatcgaa ggcaacctct accagggcga
     6301 gagagtagcc ctcgtggagg acgtcgttac cactggtacg caggccttgc gggccgccag
     6361 gaacctcgaa gagaccgggg tagaggtcgt gacgatagta gcagtactcg accgcaggga
     6421 ggaggacggg gggaaactcg ggggatttcc tttccggttc ttgcttagaa tggacgattt
     6481 acgggtagtt aaaccgaatt gaggcgctat agtgtctctg gtaggtcctt ccggcaggcc
     6541 ggagggagaa ggagtggaag cgaagcaggg aggaagtagt ggcgctgaac aagacggcat
     6601 tgatagagga gatcgccggc aaggcgaacg cctccaagag cgacgcacag aagttcttcg
     6661 acgcgttcac cgaggtcgtg accgacagcc tcaagaacgg cgagcaggtc cagatcacgg
     6721 gcttcggcaa attctacgtg cagaagcgcg acgcgaggca ggggatcaac ccgcagacca
     6781 agcagaggat caacataccg gcatccaagg tgccgaagtt caccgctggc aacgcgctca
     6841 aggaccagat caagtagccg cccaagaacg tttttggtgc cgcggggata agcccgcggc
     6901 acccttcttt tcgcccgtca gtcgacgaac cagggcgcga tgcatgcgct ggtagacgcg
     6961 gcgcgccgca agcggagcgt tctcgtcgcc ggtctcgacc ccataccctc gcgctttccg
     7021 gaagaactgc gcgtcctgcc cgaggccgaa gcgaccctaa agttctgccg gggcatcctg
     7081 gaggcggtgg agccttacgt cgccgccgta aagctgcagg cggcgttctt cgagcgcctg
     7141 ggtcccgccg ggatggggat ctacgcggac ctgatcgaag ccgctgcgga ggtcggcttg
     7201 ccgacgatag cggacgtaaa gcgcggggac atcggccccg tggccaccgc ttacgccggg
     7261 gcgcatctcg gtctctatag cgctacctgc gtcaccgtca acccttacat gggagaggat
     7321 gcggtgctcc cattcctcga ggaatcccgc agacaggacc tgggcggtgg cgtattcgtg
     7381 ctcgtcgcga cctcgaaccc gtcctcccga aaggttcagg agaccacggc cccgccgctc
     7441 ttcgaggtcg tcgccgggct cgttgccggg cttgggagcc cggccggcga ctaccccgat
     7501 gcgggggcgg tagtgggggt cacacggccc gagatcggaa gccgcgtgag agaattgctg
     7561 cccgaaaccc tattcctctc ccccggctac ggggcccaag ggggtgacgc ctccggcgtg
     7621 cgcccgttac tcgatcgtcg cggcggcgga gtcctcgtca acagcagccg cagtatcctc
     7681 tacgcctacg aaagttcggc cacgggttac aaagaagccg ccagagaagc cgcccgcaaa
     7741 gcgcgagacg aattacacct cgccggcgcc cacctgtaga gcggtcagca gtcagctttc
     7801 agccatccgc aagaacagta gtgatcggta ctgcaaagca ctaatgtaac tcaccctagt
     7861 agtcccgctt ttcttcctcc aggctgcgat cgaacgctca tactctcacg aacaaaaagc
     7921 tgaaagctta gggctgaagg ctgatagctg agagggtaat ggaaggtcga ccttccatta
     7981 ccctctaaaa caggtgggcg accgccagca ccgtcaccag caccgtcgcg gccgcgacca
     8041 cgacccctag aatctgcacc acgctctctc tgctcatctt ctccttccgg taagcccgtg
     8101 gctatggatc gggctcctgt tcgcggaggg cgctctggtg gcccttctcc ggctcactcg
     8161 cgaggatctc gtccattttc tccctgagac cgttctcgta gccttcataa tagtaatcct
     8221 gggcgtacgc tatcgtaccg tcctcgacca cgaacagcct gggcaccctc ttgacgttgt
     8281 acttggagac aagctctccc gtcgagtcca ggagcatcgt gtaggggacg ttgtcgtccc
     8341 tcggagcgct gttgacgtag accacggcga aagaggcctc atcgtcctcg tactccctgg
     8401 cgagatcttc gaagccggaa acggcctcgt tcgagtcctt gttcaaggtg ctccagaagg
     8461 tgagtacata gacaccttta tccgagagct cgaagacgcg gtcgtccacg ctcttcaccc
     8521 tgaatgcggg ggcgctgtcg cccacgctcg gggctggttg gggtagcgtg agagagcccg
     8581 taccggtctc ggcgctgccg aaggaacggt agaacaacgc ggcgaccacg acgacgaaga
     8641 cgacgatggc cagacgtttc acgaaccctc cgaagcaggt ctgaagagac acacgacata
     8701 aagattgtat ccgttaccgt tgcccttgtt gtagggtagg agacttcgta gtctctgggc
     8761 ctcgcgcggg cagcgggtat ggtataccgt tgaggggaat cttgaatgga agataggtag
     8821 ggagaggcat gatactaacc gtcaccctca acgctgccgt tgccaggacg ctggtcgttc
     8881 ccagcctgac gctcggccac aggcataggg cgcccgagag catgacgctg gccggcggaa
     8941 agggcatcaa cgtggccaga tcactgaggg ccctggacgt gcccgtgctc gccgccggct
     9001 tcgccggcgg ccgcaatggt gacgccatcc gcgacggcct ctcagaggcg gccatcccgt
     9061 tcgacctcgt cgagatagaa ggatattccc gcacttcgac ggctatcatc gaccccatgg
     9121 cgggtactca gaccgagatc aacgagtacg gccccgaagt tactcccgcc gaggccaggg
     9181 agttctccag gcgcctggag tacttgatgg agtacgctac ggccgtcgtc tttgccggca
     9241 gcgtcccgcc gaacctggac gagagcttcc tcgttggcct cgtgcggcgc gcgagagagc
     9301 gtggaatcta cacggttgtg gactcgccgc catccgtgct caggtccgcg ctcaaggcga
     9361 acccctcgct cgtaagccca aaccagcacg aggccgagag cgtcgtcggc tacgacttta
     9421 tagaggaagg ggacttccct ctcggcctca ccaggctcgt cgagcacggg gccgcgaatg
     9481 cctgcatcac ctcccccgag ggcctctcct acctcagcac cgagaacggc accgtctcgg
     9541 cgcgcgcgcc caggatcgag gccgtctcga cggtagggag cggcgacgcc tacctggccg
     9601 gactcctcgc cggtctgctg caccgcaacc tcgcgccggc cgaagccgtc cggctcgccg
     9661 cggggtgcgc cgccgccaac gccgagaccc tcggcgcagg tctcttcgag gcgcgcaggg
     9721 ccgaagagtt ggccgactcc gtgcaggtcg gaatctcttc ggttagcctc caggagagcc
     9781 ggacctaggt tgctttcgag gctccgagaa gcccgcgttg gcgtgctaga atgttcggcg
     9841 aagttcactt ccagagacta cggaggttcg agatcatggc ggtagccgaa gtaacggacg
     9901 cgactttcga gagcgacgtt ctgcggtcgg agaggccggt tatagtggat ttctgggcgc
     9961 cctggtgcgt gccttgccgg cgggtgtccc cgatcctcga agagatgtcc gagtcgcgcg
    10021 aggacgtcgc gttcgtcaag gtcaacgtgg acgacaaccc tcaggcagcg atgaactaca
    10081 gtatctcgag cattccgacc atcgcccgtt tcgaagaggg gcaggttacc cagcaggcgg
    10141 taggagcctt gccgaagaac cagctatcgg cacagctcgg cctgtaattg ccaggagcaa
    10201 cactcccggt tacaatctct ggcgcataag ctccgcggag ccgggtgagc ccggtcgtct
    10261 cgcgcggggc atcgtacaag agtccgaaga cacaaggagg tcaaacggat gaagttcaag
    10321 cctttgggag agcgggcgct cgtccagatc gtcgagcgcg aggagaccac ggcttcgggg
    10381 atagtgctgc ccgacacggc caaggagaag ccgcagaccg ccgaggtcgt cgcggtcggc
    10441 cagttcgaga acggggtcaa ggtcaccgag ggcgacgtca tcgtcttcgc caagtactcg
    10501 ggaaccgaga tcagcctcga cggcgaggac tacatgatcc tcgacgcgga cgacatcttg
    10561 gggatagtcg agagctagag gaaagagcaa gagtcggctc tttggagcgc cgggcaccta
    10621 tgcccggcgc tcgcgcgttg gccggctgcg aacactgagc gaggatagcg gggagaccgg
    10681 tcggatacgt gcgtacgctg cgtaggggag ataggggaag ggaggtcgtc gacctccaga
    10741 cccgcctgca ggcgctcggc ttcgatttgg gaaacagggg catagacggc gtcttcaggg
    10801 agaggactga gctcgctgtg agggccttcc agcagcacct cggtctcctc gctgacggtc
    10861 tcgtcggtcc catcacgtgg cgcgagatcg tggaggccgg ctacaggccg ggaggacgcc
    10921 tgctttacct caggcagccg cctttcaggg gcgccgacgt cgtcgaactc cagcagatgc
    10981 tcgacgacct ggggttcgac cccggcgccg tcaacggcct gttcgacccc cgtaccgtca
    11041 gggcggttcg cgacttccag aggaacgccg gcctacagga tgacggggtg gtcgacaccg
    11101 cggtcttcaa ggtcctgaac acgttcgccg cccagtccct cggcacccac cagctccccg
    11161 acaagagcgg aggttacttc acggaagacc tgttctcagg ggccatagtc gtggacgccg
    11221 cccacggcgg cagggatacg gggtacgaga gcccgacggg gatctccgag gcagacttga
    11281 acctcgccgt ggcgcaagag ctggcccaga tcctgccagc cagagaggtt cttctgacca
    11341 ggagcgggga cgaggaggtc tcggcggtcg acagggcctt cctcgccaac tcctcgaaag
    11401 cgaggatgat cgtctccatc caccacgcct cccaccccac cccgaccgcc tccggcacag
    11461 cctcctacta cttcggtcgg ctgggctacc agtcgcacag gggcaggatg gccgccgcct
    11521 atctgcaacg aggcatcagc caggctctgg gtacccgaga tataggggag ttcggcaggt
    11581 ctttcgagat cctgcgcgag acgaacatcc cggccgtgat cctcgagccc ctgtacctca
    11641 ccaatccggg agagctagat ctcgcctcgg atccctacta tccagcgacc gtcgccgaag
    11701 ccatagccag cgccctcgaa ctctacgccg gccgcgacaa agcattcatc gccgtgtagc
    11761 agcatttcag cacgcgccct gtcgggcgct tgtcagcagc tcagcacgct tcgggcctta
    11821 gcaggcccta gcttgtcagc aagtcagcta gggcctgtgg acatgcaggc cgaaatgctg
    11881 acaagtcgct tggcgaagcc ggagcgtgct gacaagctta accgctgaca gcgagtagcc
    11941 tgctggtata atattcgctt cagtcgggac gtagcgcagc ctggtagcgc gcctcgttcg
    12001 ggacgaggag gtcggaggtt caaatcctct cgtcccgact tccttagctg gtcagcccgg
    12061 cgcctttggc gccttgtcag cacgcttcgg gccttagcag gccctcgctt gtcagcaagt
    12121 cagcttctcc cgttccggcc tcgcctggca gcgcacaacg aaccactacc agcggcagtc
    12181 gggtcctacg ctcatgatga ggccacgacg cgacttgctg aagtgctgaa atgctgacga
    12241 gcgaaggcta aaagccagag cgtgctgaca agctgccagc ggcagcgtgc tgactagctt
    12301 tgttccgtct cttcgagtag cgcctcccag tcggcgtata gtctgtcgag agcacctttc
    12361 aattcgcggt gttcggtgac gatctcgcgg gagcgtttgc cgtcggagta gagctctgag
    12421 gtggccagtt ccttttcgag gcgggccacg cggcgctcgg tcgcgtcgat ctcgccctcc
    12481 acggcgacga ggcgggtggc gaggacgctt tgatctttgc tctggccggg cctgatgcgc
    12541 cggcgctggc ggcccttggt gcccttcgag ccctcatcga gcctgcggtg ggagcggtag
    12601 taatcgtagc caccgaggta attcctgagt ctcctgcctt ccagatcaac gaccctggta
    12661 gccagcttgc ggaggaagta gcggtcgtgg gagatgaaga acatcgtgcc cctgtactcc
    12721 aggagcgcgt cttccagggc ctcgcgggcg gggatgtcga ggttgttggt gggctcgtcg
    12781 agcaggagca tattcgcacc gctcaccacg atctcggcca ggctcagacg gttcctctcg
    12841 ccgccggaga gggacctcac cttcttgaag acatcctcgc cggagaacag gaaggcgccg
    12901 agaagatccc gggcctcggg cgcgtcgaat cccgtggctt ccatcgcctc cccaaggacg
    12961 gtcttggagt cgtcgaggcg ggccaactgc tggtcctggt aggcgagcgt aacgttgttg
    13021 ccaggcctta ccgtgcccct ctggggggag agttccccgg tggctaggcg catgatcgtg
    13081 ctcttccccg tcccgttggg accgagcagg gccaccctct ccccccgctc gatgaccagg
    13141 tcgagacctt ccaggagcgg ctcctcagcg tcatcatggg cgtagcgcac gtcctccatc
    13201 tccagcacga ctcgcccggc ccgcacggtc tccccgccga ggtagacctt gacgttcttg
    13261 gaggatctcc tggggtcctc tatctgctcc atcctgtcga gtagcttctg cttgctcttg
    13321 gcctggctgg ccttgctcgc cttggcccgg ttcttctcga caaagcgttc cagttgcgcc
    13381 ctgcgttcgg cgttggcctt cgccttgcgg gcgagttgct cctcgcggga ccgtttctcg
    13441 gccacgtact tgctgtagtt gccggggtag cgggtgagac ggccatcttc gagatccagg
    13501 atcgaaccgg cgacggcgtc gaggaagtag cggtcgtggg agacgacgag gacggcgctt
    13561 ctcgcgccct tgacgaaggc ctccagccac tctatggctc gcaaatcgag gtggttggtc
    13621 ggctcgtcga ggaggatgag gtccggctcc tccatcagca ggcgggccag ggcgatgcgg
    13681 ctctgctcgc caccagagaa cgagcccacg ggacgcttcc agtcttcggg cgagaagccc
    13741 agagaagtga tggtcgaggc cgcccgggcg cggtactcgt agccaccgtc ccgctcgaac
    13801 tcgctctgaa gcctgccgta acgctcaatg agcgtgttcg atgggtcttc ggagaggcgt
    13861 gttccaagct cctcgagctc cttctcgcgg gccatcaaag gctcgaaagc ggagatgatc
    13921 tcctcttcca aagtgaggac gccccgttct cccagataga gcttctgggc cgtcatcccg
    13981 accttcgcgc cgccaggatg ctccaccgag ccagaatcgg gttccgagtg tcctgccaga
    14041 aggtccagga gggtcgtctt gccggcgccg ttgcgcccga ccagcccgat cttctccccg
    14101 gcctcgaccg agagcgtcgc gccggagagg accttcagat ctccaccgtg gtactttacg
    14161 agatcacttg cgacaatcac cttcatggca cagagattat acaaaggaga ccacctgacg
    14221 ccggaagagc gcgaggagct agaagacgac ctcatcgcct tctgcgaccg tgaactcgac
    14281 ctcctcgggg acatcagcgg tctagacgtg ctctacgcag gaggttcctc gccgctctgg
    14341 atcgaaggcc tcagccaacg tgtgggggag aatggtagcg tcaccgcctt cgacatggat
    14401 gcggtacgcg tcgatgatac gagagcttct cttggcgagg ccggacttgc tgctccggtc
    14461 tgcctcctcg cggcagacct cttcgggatg cctttcgacc caagcacctt cgacctcgtc
    14521 tactcctcgg gcttgttcca cgagttggac gtaaagggga ggagcgccca agaggcactc
    14581 gcagcacttc acgtcatggc acggtccggt ggcaaggtcg cgacgagcga cttcgtcgac
    14641 tctgaaccgg cggtgcaact cgaggaagag aagctgcaag cagagtcgag gcgtgaggct
    14701 tatggaattg agctctacgg gatcgggccg cccgaacggc tcgtcgccct gcacgagaaa
    14761 catctcgaga acgtaaggtg gctggtatca ccaccacgga gaatccatca cctcgggaag
    14821 ctcgtcttgg ctgaggaaga gcctgcggaa ctgtccttgt tgccaccggc gacagcgaga
    14881 cggttacgcg aaagccgcga cgccttacga ggtaggatcc tgggcgaagg ctacacacgc
    14941 ccggccaccc tgtacgtcga aggtctcgta cgagaatctc gagatctcga agatcatcca
    15001 attgagtgat gcgccaatac tgcacgggcg tcatcattag tccggtgaga gtgctacgtc
    15061 ttattagaga caggaggtcc ttatgaccat cattgggctg agctccgtgg agaagttcta
    15121 cggaggcagg gcggtgctgc gaggtctggg gatgagggtg aacgcgggcg cgaggatcgg
    15181 gctcgtcggc ggcaacggcg ccggcaagtc cacggtgctt cgcatcctcg caggcgcgga
    15241 ggacgtcgac ggtggggagg tgatccggcg tcggggctcg agcgtcgcca ctctgcccca
    15301 gcacatcgag ggcgacgaac gtacaccgat ggaggtcgta cgcgcggcgc ggccggagat
    15361 ctcggatctc caggaggaac tgcaggcttg cgaggagcag ctcggctcgc ccggggtggc
    15421 gtccgacctg cgccgcatgc agcgggtatt ggagcgccag gaccggctgt tgcgccgctt
    15481 cacggagctc ggtggccccg gctttgtggg cgaggcacgc ggctacctca ggtcgctggg
    15541 tctcggtgat gacgacatct accgtccgat gagtgacctg agcggcgggc agcgtaagct
    15601 ggcggtcctg gccgcctgcc tcgcgcggcg tcccgacgtc ttgctcctcg acgagccaga
    15661 atctcacctc gacgccgggc gcagggagcg tcttgaggcg atcgtccgca cgttcgacgg
    15721 cgccgtcgtc atcgtctccc acgaccgcta cctgctggac gagacggtcg aggagatcgc
    15781 cgagctggaa gacgggagga tcacctcgtg gcccggcaac tactcggcat ataccgtggc
    15841 gcgcgagctc aagctcaaac gacagcagca gctctacgtc gcccagcaga aggagatagc
    15901 gcggctcgaa gaggccatcc ggcgcttcaa gctctgggcc agcttggtcg taaacgaacg
    15961 tcacatcaag caggcccgta acaaacaacg ccagatcgac cgcatggaca aagtcgaacg
    16021 ccccgtgctg gagcggcgca agatagggct ggagttccgc ggcagggtga ggggcggacg
    16081 gaaggtgatc gaactccaag acgtgagcgt tgccttcgac gatgagcctg tactcatcgg
    16141 ggtggacctt acggtctcgc gcggcgagcg tctcgggata gtcggaccca acggcgccgg
    16201 caagagcgtg ctcgcgaagc tcctcgccga catcctcccg ccgacggagg gcgagcgctg
    16261 ggcgggccca tccatagaca tcgggtacct ggcgcagaac gacgaaccgc caccgggcgc
    16321 ttctccactc gggctggtgc gggacgcgaa accaatctac gaaggcgacg ccgtaaagct
    16381 gctggggcgc ttcctgttcc gctacgagca ggtcagggag ccggtgacat ccctgagcgg
    16441 gggagagcga acccggctgc aattgctcct gctcatgctg agagaaccga actgcctcgt
    16501 cctcgacgag ccgaccaacc acctcgacat agactcgttg gagatcctcg aagacgagct
    16561 ggaacggttc cctggcacgg tgatcttcgt ctcgcacgac cgctacttcc tcgaccgtat
    16621 cgccgacggc atagtcgaga tcagcgaagg cgaagtgcac cgccacacag gaggctacag
    16681 cgactggcgc atgaaagcta gtcagcccgc tgccttcggc agctagtcag catttcagca
    16741 cgccgcctcc ctctggtcgg cgacttgtca gcacttcagc atgtcagcta tcgcgcttgc
    16801 gagacctggc tgacatgctg acaaggcggc gcagccgccg
//
LOCUS       DATMVF010000064         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_5950, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000064 DATMVF010000000
VERSION     DATMVF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5950"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..770
                     /locus_tag="VN178_02910"
     CDS             105..770
                     /locus_tag="VN178_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79949.1"
                     /translation="MKKSPSRARPAWVVVAVLLCGLLVSLIVFGDGGRLALGPCTPGR
                     AGAAEGVSGEAKELLETSNFEASRKAIGDLKTGVVDPRLVSTLQTVAEEHRICVDAFK
                     EGHYFLPGVPDGPLIPDSYGDAGGLPNTHYYGRAADIRRVDGRPVRGNGGNLSVLNVG
                     KTLAGIPPQERPDQIIGPSSWAEALDRSYGEGWVLNPDQLALHEDHLHIGYRSNDGTQ
                     NTR"
     gene            complement(803..1738)
                     /locus_tag="VN178_02915"
     CDS             complement(803..1738)
                     /locus_tag="VN178_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWS79950.1"
                     /translation="MAFRSVGINLPLLHLDGASRLAGDLEALKGAGPDFVELWPHHLG
                     VILGGELDPTCLRSVEETLLEADLAYTVHAPLEINLMDLNAYDLQRDVLNASIRFANG
                     VGAEVVVCHAGQRVAARDARFNFKDQLAAERFALREAGDLAGELGVTIAVENYYPELT
                     ILHGTIYDYSVWPSELAEQIAAVDHPAVGVCLDVGHAALAAGVFGFDYFEECAAAAPL
                     VRHIHLHDNLGKTNLTGVPPVSEHPVYGLGDLHLPPGRGTIPLRDIFSRVDFPEDPTC
                     CVELSPDLCSSAPQAIRAARELGELADANREQAVL"
BASE COUNT          400 a          623 c          691 g          345 t
ORIGIN      
        1 agactactcg ggtgttcgtt gtatgcagcg ccgagagaac ggtctgaagg gcgtttcgag
       61 cgttggcgcg gtatgaaccc agaaggagtc gagcagggta ccgcgtgaag aagagcccga
      121 gccgcgcccg accagcctgg gttgtggtcg cggtcttgct ttgcggcctc ctggtgtctc
      181 tgatcgtctt cggggacggc ggaaggcttg ctttggggcc gtgcacgccg gggcgtgcgg
      241 gggccgccga aggcgtaagc ggcgaggcca aggagcttct ggaaacctcc aacttcgagg
      301 cctcgcgcaa ggcgataggg gacctcaaga ccggggtcgt ggatccgcgc ctcgtgagca
      361 ccctgcagac cgtggctgag gagcaccgga tctgcgtcga cgctttcaag gaaggccact
      421 acttcttgcc gggcgtcccg gacggcccgc tgatccccga tagttacggt gacgccggcg
      481 gtctaccgaa cacgcactac tatggcaggg ccgcggacat acgcagggtg gacggcagac
      541 ccgttcgagg caacggcggc aacttaagcg tcctgaacgt cggtaagacc ctcgccggca
      601 tcccccctca agagaggccc gaccagatca taggcccgtc gagctgggcg gaggccctgg
      661 accgctcgta cggggagggt tgggtcctga accccgacca gctggcgctc cacgaagacc
      721 acctccacat cggctatagg agtaacgacg gaacccaaaa cacgcggtga gcacgaaggc
      781 gcaagtaggt caggtctgtg atttagagga ctgcttgctc ccgattcgcg tccgccagtt
      841 ctccgagctc gcgggcggcc cgaatggcct gcggggccga agagcagagg tcgggagaga
      901 gctccacgca gcacgtggga tcttcgggga agtccacccg cgagaatata tctcgaagcg
      961 ggatggtccc tctacccggc ggcaggtgca gatcccccaa cccgtagacg gggtgctccg
     1021 agaccggagg cacgcctgtg agattggttt tgcccaggtt gtcgtgcaga tgtatgtggc
     1081 gaacgagggg tgctgcggcg gcacactcct cgaagtagtc gaagccgaag acgcccgccg
     1141 ccagggccgc gtgtccgacg tccaggcaga cgccgaccgc gggatggtcc accgccgcga
     1201 tctgctccgc cagctccgag ggccagacgg agtagtcgta gatcgtaccg tgaaggatag
     1261 ttagctctgg gtagtagttc tcgacggcga tggtcacgcc gagctcccca gccaggtctc
     1321 ccgcctccct gagggcgaac cgctcggcgg cgagctggtc cttgaagttg aagcgggcgt
     1381 cgcgcgccgc cacccgctgg ccggcgtggc agaccaccac ctccgcgccg acgccgttcg
     1441 cgaaccggat gctggcgttc aagacgtcgc gctgaaggtc gtaggcgttc aggtccatga
     1501 ggttgatctc cagcggggca tgcacggtgt aggccagatc cgcctctagc aacgtctcct
     1561 caacactccg taagcatgtt gggtccagtt cgcccccgag gatgacccct aagtgatgcg
     1621 gccagagctc cacaaaatcc ggcccggccc ccttcagggc ctccaggtct ccggcgagcc
     1681 tggaggcgcc gtccagatgc agaagcggca ggttgatgcc cacgctccga aaagccatac
     1741 gatgacttcc tcccttagaa cggtagacgc gtcagaatca taaaccgcgg ccagtagtat
     1801 atggggtagg agcgttcctt gagtaaaaag agcgttaacg gagggtaaac cttggtttaa
     1861 ctggccctaa agaacgctct cgtgtaccct cctgggagga aaagcgagcc cattagggag
     1921 gtttagatga gtagctacga gaactacacg cggacgtcca ggaactacga caggacgcgg
     1981 cagccgatag gcgccgagat catcgtcggc tgtttagccc aggcgccggt gcccctggat
     2041 cggacggtgg tgttggatg
//
LOCUS       DATMVF010000065         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_469257, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000065 DATMVF010000000
VERSION     DATMVF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469257"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VN178_02920"
     CDS             <1..457
                     /locus_tag="VN178_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWS79951.1"
                     /translation="AFGEVDVHELFRPDGAGTHAAPAAFTAGSGDPHDVGDTHSVSVT
                     FDGAIDWTAFGIWFSMLLNARGEDVLRVKGLLDVGEAGPVVINGVQHIIHPPEHLGEW
                     PDEDRQSRIIFITRYIHPEELLDSLLAFRGLLGARPVPLHAAEQRVPFL"
     gene            complement(524..970)
                     /locus_tag="VN178_02925"
     CDS             complement(524..970)
                     /locus_tag="VN178_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79952.1"
                     /translation="MIGWRVGDPPRTSEHILRMDPTLDLERRRLLISAALTAQARRRL
                     GSSLAANLSVGKLLDLACGVSEGAGTIERAYRLRFDPPYPGVTAGPQSVGGTSRLMLA
                     CSVRDQVGTPLGVVFTTLIAGRPPQVSVAPAGASIPEGWRSPGDFH"
     gene            1018..>2115
                     /locus_tag="VN178_02930"
     CDS             1018..>2115
                     /locus_tag="VN178_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWS79953.1"
                     /translation="MSAPRNLTRKLLGDHLLEGNLVPGEEMDLIVDQILIEDATGSMT
                     ALQFEALGADNVAVPLAVMYVDHNVLQIDDKNMDEHRFLQSFSSRYGIQYSRPGNGIS
                     HYVHLERFARPGELLVGADSHSTMAGAVGMFAVGAGGLDVAVAMAGYGFSLECPKVVG
                     VELRGELPNWVQSKDIILELLRRYGVRGGVGRVFEFTGEGVSTLSVTDRGTICNMITE
                     IGATAAVFPSDAQTSEWLAAQRREEDYVPLAADPKASYDENEVIELSQLEPLIAEPSS
                     PGNVVPVCEVAGTQTVQVCVGSSVNSSYEDLATAAAILRENIVHPRVEMTVTPGSRQI
                     LDTISRSGVYQDLVAAGARMLEPVCGPCIGVG"
BASE COUNT          363 a          685 c          690 g          377 t
ORIGIN      
        1 ggctttcggc gaggtggacg tccacgagct ctttcgtccg gatggcgcgg ggacgcacgc
       61 ggcgcccgcg gcctttactg cggggtccgg cgatcctcac gacgtgggcg acacgcactc
      121 cgtctcggtg acgttcgacg gggccatcga ctggacggcg ttcggaatct ggttcagcat
      181 gctgctgaat gcccggggtg aggacgtgtt gcgtgtcaag ggactgcttg acgtcggcga
      241 ggcagggccc gtcgtcatca acggcgtgca gcacataatc catccgccgg agcacctcgg
      301 cgagtggccc gacgaggacc gccagtcccg gataatcttc atcaccaggt acatccatcc
      361 cgaagagttg ttggactccc tgctcgcttt ccgcgggttg ctcggagcac gacccgtacc
      421 cctccacgcc gccgaacaga gagtgccgtt cctctagtcg cttgaaggat ctcgcaaaat
      481 ggtcgtggga ccatcagcca ctccggtcga gtggcgtggg gtctcagtga aagtcccccg
      541 gcgaccgcca accttcggga atgctcgctc cggcaggcgc caccgacacc tggggaggcc
      601 ggccggcgat gagcgtggta aagaccacac caagaggagt tccgacctga tcgcggacgc
      661 tgcacgcgag catgagcctg gaggtccccc cgacgctctg gggtccggcc gtgacgccgg
      721 ggtagggcgg atcgaagcgg agacggtacg cgcgctcgat cgtgcccgct ccctcgctca
      781 ccccgcaggc gagatccagg agtttcccaa cgctaaggtt ggcagcgagg ctgcttccca
      841 ggcgccgccg ggcctgcgcg gtcagcgccg cggatatgag gaggcgtctc ctctcgaggt
      901 cgagggtagg atccatgcgc aagatgtgct cgctcgtacg tggcgggtcg cccactctcc
      961 agccgatcat tctaactata ccgtctgtac cttgttgcat acagacagga ggcgaacatg
     1021 agcgcgccgc gcaatcttac ccggaagctg ctgggcgacc atctgctaga aggcaacctc
     1081 gtccccgggg aggagatgga tctcatcgtg gaccagatcc tgatcgagga cgccactggc
     1141 tctatgaccg ccctccagtt cgaggcgctc ggagcagata acgtcgccgt ccccctcgcg
     1201 gtgatgtacg ttgatcacaa cgtcttgcag atcgacgaca agaacatgga cgagcatcgc
     1261 ttcctgcagt ctttctcgtc ccgctacggc atccaatact cccgccccgg caatgggatc
     1321 tcccactacg tccacctcga acgtttcgcc agacccggcg agctcctggt aggcgccgac
     1381 tcgcactcca ccatggctgg cgcggtcggt atgttcgccg tcggggctgg gggtctagac
     1441 gtcgccgtcg ccatggcggg gtacggcttc tctctggagt gcccgaaggt cgtgggcgtc
     1501 gagctgcgcg gagagttgcc caactgggtg cagtcgaagg acataatcct cgagctcttg
     1561 agacgctacg gggtacgcgg gggcgtggga cgggtcttcg agttcacggg ggagggtgtt
     1621 tctactcttt cggtgacgga tcggggtacg atctgcaaca tgatcacgga gattggagcc
     1681 accgccgccg tcttcccgag cgacgcgcag acgagcgagt ggctcgccgc ccagaggcgc
     1741 gaggaggact acgtcccgct cgccgcagac cccaaggcct cttacgacga aaacgaagtg
     1801 atcgagctct cgcagctcga gccgctgatc gcggaaccgt cctctccggg caacgtggtg
     1861 ccggtttgcg aggtcgccgg cacgcagacg gtccaggtgt gcgtcggctc ctcggtgaac
     1921 tcttcctacg aagacctcgc caccgccgcg gccatcctgc gggagaacat cgtgcatccc
     1981 cgcgtagaga tgaccgtcac gccggggtcg cgccagatcc tggacaccat ttcgaggagt
     2041 ggcgtctacc aagaccttgt cgccgctggc gcgcgcatgc tcgagcccgt ctgcggtccc
     2101 tgcatcggcg tagga
//
LOCUS       DATMVF010000066         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_284774, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000066 DATMVF010000000
VERSION     DATMVF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284774"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VN178_02935"
     CDS             complement(<1..186)
                     /locus_tag="VN178_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79954.1"
                     /translation="MTFSALFKMWATVLAAVLAATLLSAAAFTPGAGAQETGCKGQVV
                     LDAGHGGTDSGAVNTRYG"
     gene            complement(212..832)
                     /locus_tag="VN178_02940"
     CDS             complement(212..832)
                     /locus_tag="VN178_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79955.1"
                     /translation="MDRGEEIIGLNGAAAVLDVPPETVRRFAQEGRLRPEGGEEGGYV
                     FQLEDVLGLARELGAESAEEEIFEGEVMGDEPAGNSRLPAVPFERYERLLQQGQDYKT
                     RLEERTRQIEEIRQERDAARMEIDRLWSEIERLNLVEFQRELQEKTISTLEEEKATLQ
                     AERERLLEEQMNLTEEKSALSEDRVRLEAELNAMKNRGFWARLFGG"
     gene            complement(947..1432)
                     /locus_tag="VN178_02945"
     CDS             complement(947..1432)
                     /locus_tag="VN178_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWS79956.1"
                     /translation="MERISRERFIRLGSALGVGAACASLVACGGGASSSGGSGGGNGG
                     GGDSGGGGTKKSSGGGAKAGGTAIVSESEVAPGSAHKFEDSGNPAVLVHLDNGEFVAY
                     SAVCTHQGCDVAYKDGNLACPCHGSIFDPADGAAVVAGPAQRPLPEIPVKVEGGEVVR
                     A"
     gene            1810..2292
                     /locus_tag="VN178_02950"
     CDS             1810..2292
                     /locus_tag="VN178_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HWS79957.1"
                     /translation="MVLVRVLIGAVWLNGGLEKLLNPGFPGQFAASLEAGGFVSQAPP
                     FFQGFMRGYVVPNAELFAQFMRVGELMLGIALILGLLTNLAAIGSVALSAVILLSQGG
                     VGLGTGLGAPEFLTINVLVALFSVVILFSPAAKSLSLDSRLAERRPVLSPILINRRQN
                     "
     gene            complement(2297..>2643)
                     /locus_tag="VN178_02955"
     CDS             complement(2297..>2643)
                     /locus_tag="VN178_02955"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79958.1"
                     /translation="TGLREQATILDSILARGGEPIVLDVRELLLDPPDVLRRVCSRLG
                     IPFDKAMLSWPAGPKPEDGVWAKHWYHNVHASTGFAPYQPRKDPFPAHLKPLLEECLP
                     LYERLRDYAIGA"
BASE COUNT          405 a          889 c          805 g          544 t
ORIGIN      
        1 gccgtatctg gtgtttaccg ctcctgagtc cgttcccccg tggcctgcgt ccaggaccac
       61 ttgtcccttg caaccggtct cctgcgcccc cgcgccaggg gtaaaagcag ccgcagagag
      121 tagggtcgcg gcgagaactg ctgccagcac cgtcgcccac atcttgaaga gagccgagaa
      181 tgtcatcccc catccttccc cataagattc ttcagccgcc gaagaggcgg gcccagaagc
      241 cgcggttctt catggcgttg agctcggctt cgaggcggac ccggtcttcg gagagggccg
      301 acttttcctc cgtaaggttc atctgttcct cgaggagacg ttcgcgctcg gcctgtagcg
      361 tggctttctc ttcttcgagg gtcgagatgg tcttctcctg cagttcgcgt tggaactcca
      421 cgaggttgag gcgttcgatc tcggaccaga ggcggtcgat ctccatcctc gccgcgtcgc
      481 gctcctggcg gatctcttct atctggcggg tccgctcttc gaggcgggtc ttgtagtcct
      541 ggccctgctg caggaggcgc tcgtagcgct cgaacggcac ggcagggagc ctggagttgc
      601 cggcgggctc gtcccccatc acctctccct cgaagatctc ctcctccgcg gattcggcgc
      661 caagctcacg cgcgagcccg aggacatcct cgagttggaa gacgtaccct ccttcctccc
      721 cgccctcggg gcgaagcctt ccctcctgcg cgaagcgcct gaccgtctcg ggcgggacgt
      781 cgagaacggc cgccgccccg ttgagcccga tgatctcctc gcccctgtcc atgggagcat
      841 tttactttag ctttcagcgg tcagccatca gccgtcagct tttcggtgga cagagaacgt
      901 cttgccggga aggtgggacc gccctggggt ctctagctcg ctgctctcag gcccttacca
      961 cttcgccgcc ctcgaccttc accgggatct cgggcagcgg cctctgggcc gggccagcaa
     1021 ctaccgcggc gccatctgcc ggatcgaaga tcgatccgtg gcacgggcag gcgagattgc
     1081 cgtccttgta ggccacgtcg cagccctggt gcgtgcagac cgccgagtat gccacgaact
     1141 ccccgttgtc aaggtggacc aggacagcgg gattccccga gtcctcgaac ttgtgggcac
     1201 tccctggcgc gacctccgac tcgctcacga tggccgttcc acccgccttg gccccaccgc
     1261 cggacgactt cttcgtgcca ccgccacccg agtccccgcc gccaccgttc ccgccgcccg
     1321 aacctccaga gctgcttgcg cctcctccgc aggcgaccag cgaggcgcag gccgcgccca
     1381 ctccgagcgc gctgcctagc cggatgaacc tctcgcgcga aatcctctcc aagactccct
     1441 cctcgtcgtc gccgggtctc cctgatatac gcagcgcacc cggcatcggt tgtgttcagc
     1501 ttgtcagcac gctccagcct ccggctgtcg cttgtcagca tttcagcacg tcgcctgttc
     1561 caggcgactt atcagcactt cagctggtca gcatttcggc tagatcctaa actctctctg
     1621 gtgcgtatga aggatagcga agcgtagcag tttggcagtg ccgtgcccaa ggcttcggcc
     1681 ttaccttcga tgagccgtcg gcaaacagaa agctgaccag ctgaaacgct gactagctga
     1741 ttagcgcggt gtttggaagg gtcaaccctt ccaaacaccg cattgagagg agtttggcac
     1801 gtgaacggca tggtgttggt gcgcgtcttg atcggggcgg tctggctcaa cggtggcctg
     1861 gagaagctct tgaatccggg ctttcctggc cagttcgcgg cgagcctgga ggcagggggg
     1921 ttcgtctcgc aggctccgcc tttcttccag ggctttatga ggggctatgt ggttcctaat
     1981 gccgagctct tcgcccagtt catgcgtgtg ggagagctga tgctcggcat cgccctcatc
     2041 ctcgggttgc ttaccaacct cgccgcgatc ggcagcgtgg ctcttagcgc cgtgatcctg
     2101 ctctcccagg gtggcgtggg gctgggaacg ggcctcggcg cccccgaatt cctcacgatt
     2161 aacgtcctcg tggccctctt ctcggtggtg atcctgttca gcccggccgc gaagtctctc
     2221 tccttagact cccgcctcgc cgagcgcaga cccgtactct caccgattct catcaaccgt
     2281 cgccagaact aaccgctcag gcgccgatgg cgtagtcgcg tagccgttcg tagaggggca
     2341 ggcactcctc taacagcggc ttcaggtggg ccgggaacgg gtccttacgg ggttggtagg
     2401 gcgcaaagcc ggtcgaagcg tgaacgttat ggtaccaatg cttggcccac acgccgtctt
     2461 ccggcttcgg tccggcgggc caggagagca tcgctttgtc gaagggtatg ccgagcctgg
     2521 agcagacccg gcgcagtacg tccggtgggt cgaggagtag ctcgcggacg tcgaggacga
     2581 tgggttcccc gccgcgggcc aggatcgagt cgaggattgt ggcctgctcg cgcagaccag
     2641 tgt
//
LOCUS       DATMVF010000067         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_649083, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000067 DATMVF010000000
VERSION     DATMVF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649083"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VN178_02960"
     CDS             complement(<1..821)
                     /locus_tag="VN178_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWS79959.1"
                     /translation="MEKPVGSGRTAYTVIGLVFALLVGGYFVYRVSAVVLAFLLTILL
                     SIILSAPVNYLARRGWPRTWGALTVVTALVAVLWLFGLALVPAVETQSRQFAEAFPTL
                     LEEAFALANRLQSFFGLGTQIGLDPESLSGVAREFLTGSTVSTAAGVGLTAATVVSFG
                     VVVFISTIYLVIRPEPWVNGFVSLFPAEWRQRTREVLQTLYHTVQRWFLGQLAAMTFI
                     AVFWAISLSLIGVPFALLLGIFSGLISFIPYLGALISVVLPVLLALISDPFSTVWV"
     gene            complement(1100..1882)
                     /locus_tag="VN178_02965"
     CDS             complement(1100..1882)
                     /locus_tag="VN178_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS79960.1"
                     /translation="MMENGSANGSDYLRVEGVIKRFGAISVLKGVDLHVGEGEVLGLI
                     GDNGAGKSTLLKTITGFHRPDDGRILIEGEEVNLRSVSQARSLGIQTVYQDLALVNEL
                     SVFHNMFLNGEMTVGPFLNNRKMKEKTSQYLADMGVNIPSIDTEVARLSGGQRQAIAV
                     ARAVYSDAKMLLLDEPLAAMGAKEGALILDLIQRLKEERGIPMILIVHNYAQVFDVCD
                     RVNLLRNGRIEYDKPVAESSVEELTELVVSEYRKARETGNGG"
     gene            complement(1879..2889)
                     /locus_tag="VN178_02970"
     CDS             complement(1879..2889)
                     /locus_tag="VN178_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS79961.1"
                     /translation="MSESSAQVSTGTQERGFGSRLLGGLVRVREVSILIVLVGLALYF
                     QTANPAFLSPQNILSLAQFAVPYVVIASALCLLLICGEIDLSVGQSFAFAPIVMYLAY
                     DKLFLVLPLAVLVGLLAVAVVGLINGLITVYLGVSSLITTLGMFFLLAGLNVTLTGGF
                     PAITPETSSLTQVLGESSWAGTIWALIIVAVLQLVLSRTRWGLHTYATGGNPLGAREA
                     GVSIARIKVGNFILCGVLGGFVGIIEAFRINSIDPLAGGPDIVLLCIASAVIGGTALL
                     GGVGTVTGAFLGALVLTILRNGFTLQGISAYTFNIILGVAILVAMILNVYVARLRGAG
                     RA"
     gene            complement(2915..>3967)
                     /locus_tag="VN178_02975"
     CDS             complement(2915..>3967)
                     /locus_tag="VN178_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWS79962.1"
                     /translation="GRRQFLAGSALASAALLLAGCRQEAAQQASQGGGGGSGGNFPDT
                     PEWNFVFVNHVTTNPFFVPTQYGIEDAQALLGTTSQWTGSEESVVSEMVDAMNTAISG
                     NADGIAVCVVDPVAFNKPIQKALDNDIPVIAYNANGKGTGTNPALAYVGQDLFLSGVE
                     MGKRIVSLVDEGLVALFIATPGQLNIQPRIDGAKQAIEDSGANIEIQQITTSPELPEE
                     RSRIEAWYNGHKDVAGMFAVDAGSTQGVAQVMEQFGLHEKGVRAGGYDLLPETLKLIK
                     AGHMDFTLDQQPYLQGFFPVMQLYLYKISGGVTGPAETNTGLVFITPDTVDRYLETTS
                     RFEGDSEAQKIVKAPS"
BASE COUNT          760 a         1237 c         1266 g          704 t
ORIGIN      
        1 acccagacgg tcgagaaagg gtctgagatc agagccagaa gcactggcaa gacgaccgag
       61 atgagcgcgc ccaggtaggg gatgaacgag atcaaaccgc tgaagatgcc gaggagcaac
      121 gcgaaaggca ccccgatcag ggagagcgag atggcccaga aaacagcgat gaaggtcatg
      181 gccgcgagct ggccgaggaa ccaacgctgc accgtgtggt acagcgtctg aagcacctct
      241 cgcgtcctct gccgccactc cgcggggaag agcgagacga acccgttgac ccagggctcg
      301 ggcctgatga ccaggtagat ggtggagatg aacacgacca ccccgaacga gaccacggtc
      361 gccgcggtca gcccaacccc tgcggccgtc gatacggtgc ttcctgtgag gaactctcgc
      421 gcgaccccgg acaggctttc ggggtcgagt ccgatctggg tgccgagccc gaagaagctc
      481 tggaggcggt tggccagggc gaaggcttct tccaggagcg tcgggaaagc ctcggcgaac
      541 tggcgggatt gcgtctcgac ggcggggacc agcgcgagac cgaagagcca gagcactgcc
      601 accagcgccg tgaccaccgt caaggctccc cacgtcctgg gccacccccg ccgggcgagg
      661 tagttgaccg gggcgctgag tatgatggag agcaggatcg tcagcaggaa cgccagtacc
      721 acagcgctga cccggtagac gaaatacccg cccacgagca gggcgaagac cagcccgatg
      781 accgtgtaag ccgttcttcc cgagcctacg ggtttctcca ctgctatccc ttcggccgtc
      841 tctatggccg gagtttactt gttcgtggcg tgttggggaa ggattaatcc ttccccaaca
      901 cgcgcatgtc agcacgctca ggcttttagc cttcgcttgt cagcccgccg accggcttcg
      961 ccgagcggct tgtcagcatt tcagccaaga cttgcgcaag agaaagctga catgctgatg
     1021 tgctgacaag cgcccgacag ggcgcgtgct gacaaggcgc caacggcgcc gtgcgggaaa
     1081 cggcgggtag ggcgacgccc tacccgccgt ttccggtctc tcgggctttg cggtattcgg
     1141 agacgacgag ctcggtgagc tcttcgacgg agctctcggc gaccggcttg tcgtactcta
     1201 tgcggccgtt tctcaggagg ttgacccggt cacagacgtc gaagacctgg gcgtagttgt
     1261 ggacgatcag gatcatgggt atgccccgct cctctttgag gcgttggatg aggtccagga
     1321 tcagggcccc ctccttggcc cccatcgccg cgagcggctc gtccaggagc agcatcttcg
     1381 cgtccgagta gaccgcgcgc gcgacggcta tggcctggcg ctgcccgccc gagagtctcg
     1441 ccacctcggt gtctatggaa gggatgttca cgcccatgtc tgcaaggtac tgggaggtct
     1501 tctccttcat cttgcggttg ttcaggaatg gtcctacggt catctcgccg ttcaagaaca
     1561 tgttgtggaa gacagagagc tcgttgacga gcgccaggtc ctggtagacg gtctggatgc
     1621 ccagagagcg cgcctgggag acggatcgga ggttgacctc ctcgccttcg atcaggatcc
     1681 tcccgtcgtc gggacggtgg aagccggtta tggtcttcag cagcgtgctc ttgccggccc
     1741 cgttgtcgcc gatgagcccg aggacctctc cttcgccgac gtgcaggtcg acgcccttga
     1801 gtacggagat cgccccgaag cgcttgatga cgccctcgac gcgcaggtag tccgagccgt
     1861 tggcggagcc attctccatc atgccctccc cgcgccgcgc aatctcgcga cgtagacgtt
     1921 gaggatcatg gctacaagga tggcgacgcc aaggatgatg ttgaaggtgt aggcgcttat
     1981 gccctgcagg gtgaagccgt tgcggaggat ggtaagcacc agtgccccga ggaacgcgcc
     2041 cgtaacggtc cccaccccac ccagtagcgc cgtcccccct atgaccgcag aggcgatgca
     2101 caggagaact atgtctggtc cgccggcgag cgggtctatg gagtttatgc ggaaggcctc
     2161 tatgatgccc acgaaaccgc cgagcacgcc acagagtatg aagttgccga cctttatgcg
     2221 tgcgatgcta acaccggcct ccctggcccc gagcgggttg ccgccggtgg cgtaggtgtg
     2281 cagcccccac cgggtgcgcg agaggacgag ctgcaagaca gccacgatga tcagggccca
     2341 gatggtcccc gcccaggagg actcaccgag gacctgagtg agggagctcg tctccggcgt
     2401 gatcgcgggg aagccgccgg tgagcgttac gttgagtccg gcgagaagga agaacatccc
     2461 cagcgtggtg atcagcgagg aaacgccgag gtagacggtg atcaggccgt tgatcaggcc
     2521 gactacagcc acggccagga gcccgacaag gacggcgagc ggcaagacca ggaagagctt
     2581 gtcgtaggcc aggtacatca ctatgggcgc gaacgcgaaa gactggccta cggagaggtc
     2641 gatctcaccg cagatgagca acagacacaa cgcggacgcg atgaccacgt aaggcacggc
     2701 gaactgggcg agcgagagaa tgttctgcgg ggagaggaag gcggggtttg ccgtctggaa
     2761 gtagagcgcc aaccccacga ggacaatgag gatgctcacc tcccttaccc ggacgagccc
     2821 gcccaacaac cggcttccga aaccgcgctc ctgcgttccg gtactcacct gtgcggagct
     2881 ctcgctcaag tctcctcctc ccgagggttg ctacctaaga aggcgccttc acgatctttt
     2941 gcgcctcgga gtccccctcg aaacgcgacg tggtctcgag gtagcggtcc accgtgtcgg
     3001 gcgtgatgaa gacgaggcca gtgttcgtct ccgccggtcc cgtgacccct ccagagatct
     3061 tgtaaaggta gagctgcatg acggggaaga agccttgcag atagggctgc tggtcgaggg
     3121 tgaagtccat gtgcccggcc ttgatgagct tgagtgtctc cggaaggaga tcgtacccac
     3181 cggccctgac gcctttttcg tgcaggccga attgctccat tacctgggcg acgccctgtg
     3241 tgctccccgc gtccacggcg aacatgccgg ccacgtcctt gtggccgttg taccaggctt
     3301 cgatcctgga cctctcttcc ggcagctcgg ggctcgttgt gatctgctgt atctctatgt
     3361 tcgctcccga gtcctctatg gcctgcttcg cgccgtcgat cctcggctgt atgttgagct
     3421 gaccaggcgt cgctataaag agcgccacaa gcccttcgtc gacgaggctg acgatccgct
     3481 tgcccatctc cacgccggac aggaagaggt cctggcccac atacgcaagc gcagggttgg
     3541 tccccgtccc cttgccgttg gcgttgtagg caatgacggg aatgtcgttg tccaaagctt
     3601 tctggatcgg cttgttgaaa gccacgggat ctacgacgca gacggctatg ccgtcggcgt
     3661 tgcccgagat ggccgtgttc atcgcgtcga ccatctccga gacaactgat tcctccgacc
     3721 ccgtccactg tgacgtagtc cccagtagag cctgcgcgtc ctcgataccg tactgggtcg
     3781 gtacgaagaa cgggttggtg gtcacatggt tgacgaagac gaaattccac tccggcgtat
     3841 ccggaaagtt gccgccagac ccgcctccac cgccctggct cgcctgctgc gcagcctcct
     3901 gcctacaccc cgcgagcagc aacgccgcgg acgccagtgc gctccccgcg agaaactgcc
     3961 tgcggcc
//
LOCUS       DATMVF010000068        10781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_147547, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000068 DATMVF010000000
VERSION     DATMVF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147547"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VN178_02980"
     CDS             complement(<1..658)
                     /locus_tag="VN178_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79963.1"
                     /translation="MTTDETSNTAGPQDSLRGFPLPGSAASVVAVPAPGDGPGYWAGA
                     SSAALDEDGTFLIGYRLRHGHDGAAETVIARSDDGERFTTVATLEQSEFGAKSMERPS
                     LLRTETGSWRLYVCCADPGSAHWWIEAIDADEPEGFAAEGARTIFPGDRLTGVKDPLA
                     QRTGQGWRAWICCHLLDQPGEEDRMNTAYATSEDGLSWEWHGVALEGRPGAWDARGAR
                     V"
     gene            1028..1183
                     /locus_tag="VN178_02985"
     CDS             1028..1183
                     /locus_tag="VN178_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79964.1"
                     /translation="MSDQTTEPSVVEGAHLQKLPKRRRISTVSFVGAIEVGPFVGAWP
                     GLDQDLA"
     gene            1280..2152
                     /locus_tag="VN178_02990"
     CDS             1280..2152
                     /locus_tag="VN178_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79965.1"
                     /translation="MQPASDIVAPETLGFESSPEGWWKTVGERRLLFMRLRSRLDLEK
                     TERLQRSVFGVTDRDLASSSILVAVHETGGEVIGAFDGEQLVGFVSGWGGYVDGHARL
                     VSDLMAVESGYRGGIGFALKGLQAIIAFQAGFEEIVWTVDPLRAANARLNLERLGAHS
                     RKYLRNLYGEHFAEGLYGGLPSDRLLLTWPLRSARVRSRLLGRYRPLEPGALATLPEY
                     DSGTSERRVRLAIPGDFDGLLSSDPARAREWRYRLRDNLEAAFASGYVITGFAGRRGE
                     PYGYYVLERDFVFE"
     gene            2206..3327
                     /locus_tag="VN178_02995"
     CDS             2206..3327
                     /locus_tag="VN178_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HWS79966.1"
                     /translation="MRITGVEIVPVKLPLLEPFVVSYGTFPDLATVLVRLETDEGLTG
                     WGEGTPDPHVTGETFEGVVATLRLLAPALLGHDPLDRSTAMRLLKSRVVGVPTAKAAL
                     DIALHDLAGRVAGLPVWTLLGGRAREALSISRVISLKSPEAMAIDANQFVAAGFRTVK
                     LKVGDAHDIRGDVRRVAAVREAVGPDIGIKIDVNGGWRTAGAAVGAVRGIAAYDPEYV
                     EQPVDRRDLEGMAEVRRLGGVQVMADEAVLDAHDALQAVRLRACDLINIKLMKCGGLL
                     AALALDAVAETAGVGCQIGTMVESSVASAAGLHLALALHNAATVEMGGPIMLAEDVGD
                     LRAYYEQHRVTVPAGPGLGVEPEEAVLKRYAGQRDWITV"
     gene            3443..4180
                     /locus_tag="VN178_03000"
     CDS             3443..4180
                     /locus_tag="VN178_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWS79967.1"
                     /translation="MFADKGELSPIYRAVAEALIERIDDGDLRPGSRCPSERQLASEY
                     GISRMTARAAVNLLVQRGYVARKNGSGTFVASPKIELDLSTVAGFSDRVLRHGIVPGA
                     RVIEARTVSNEELDASVATALEITGEELVHVLVRVRTGNNELLALERSYFPARHCPDL
                     LDNELTGSIYELLQTRCNLELAHLRQKLEVTQLDASAAEALATHPDAPALRITRTTWD
                     AHGRPIEFARDLYRGDRLLFVTEAPAS"
     gene            4537..5457
                     /locus_tag="VN178_03005"
     CDS             4537..5457
                     /locus_tag="VN178_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWS79968.1"
                     /translation="METSDPALKEVVNSMPAWASADGVEAEYISGGITNRNYCVRVDG
                     EPFFVRLAGEETEALGINRENERSAIEAAAAAGMGPEVVAFLPEHGCLITEWIEGAPV
                     PPEDLRQRTMLDRLVRSIRAFHGCSEIPGTFSPFRVVEVYRESAESRGVEVPEIYWWL
                     LERAREVEEAFQQEPTPSRPCHNDLLNANFLLCDDRVMIVDYEYAGMGDVFFDLGNLS
                     VNNDFDEDADVALLELYFGAVTPSRVARLKLMRLMSDFREAMWGVMQQAISTLDFDYE
                     DYANQHFARCREHAEDERHPMWLRDAAKKT"
     gene            5578..6885
                     /gene="eutH"
                     /locus_tag="VN178_03010"
     CDS             5578..6885
                     /gene="eutH"
                     /locus_tag="VN178_03010"
                     /inference="COORDINATES: protein motif:HMM:NF011668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HWS79969.1"
                     /translation="MAIIGDVVVYIIMAFVVAGAIAAIRDDEHGMGKEFKEGLHAIGP
                     IFIPVAGIMASIPYLSQFIKAVVGPVFGLVGASPAMASTTVIAVDMGGYQLAEATANS
                     TANWIMAAVVGYMAGATIVFSIPVGLAMLDQRDHKYMALGIMSGILTVPLGVLITTSI
                     LSITNGQVRDEVSTTTNSDYALNDLTFGMIFANLFPLAIIMIAIALGLYFYANAMIKG
                     FMWFGKAMDTGIKLVLAFSIVEYFTGFFSLVFGSWGFDPIIADAEDQFRALEIAGYIG
                     ITLAGAFPMVYVIRTYLAKPLGAVGRRFGISAEGSAGILAAAANILALYHLIRRIPPK
                     DKVLCIAFAVCSAFLLGDHLAFTANFQPNMILPLMLGKVGAGIIAIALAYWIAVPTAL
                     ELERRDREEGVIGEDEYPSVGASRAGEEESVEEVATGGREERE"
     gene            6970..9399
                     /locus_tag="VN178_03015"
     CDS             6970..9399
                     /locus_tag="VN178_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS79970.1"
                     /translation="MNEEAQAVIIGGGVGGASIAYHLTGIGWQDVVLVEKSELTHGTT
                     FHSAGLVGQLRPTETLTRMNMHSVELYRRLQKETGADPGWKEVGSLRLVSSRDRMEEL
                     TTLVGRAKTFGLPLELIGTEEALELFPLFDPRGVLGAAYDPTDGYIDPSALTQALAEG
                     ARLRGAKIHTNVRVTGITVDNGRVKEVATDHGVIRTEVVINATGIWAPEIGKLVGVNV
                     PLIPFQHQYVHVKPEEPISPDFPTMRDPDDLVYFRPHEGDIMTGGYGRHPAPFALDGV
                     PADFGHKLLEPDWGRFSPLFEAACARVPAIRDGEVVQLTNGPESFTPDGEFILGESHV
                     RGFFVAAGFNAHGIAGAGGMGQIMAEWIVEGEPSMDVWQMDIRRFGEHYRSRRYTLER
                     SSEALSTYYDIHFPNEESRSARGLRLSPAYERLRALDAEFGEKAGWERPNYFRSNEDP
                     AYERLRPRGWAGRLWSTAIPAEHLATRERAGLFDQTSFAKIEIEGPGACALLQRLCDN
                     DVDKPPGTVTYTQMLNPRGGIECDFTVTRLEDERFRIVTGTAFGIHDMDWIRRHQPDD
                     GSVQIRDVTSSLACIGLQGPRARDILSSVCQDDLSNEAFPYMRAREITVQEVPCLALR
                     VTYVGELGWELYPPMEFGTRLWDVLFEAGRPHGLVPAGYRAIESMRLEGGFLAWAADI
                     TPEDNPYEAGLGFAVKLDKSMPFIGKEALEKIKAQGTQRRLRCLVLADQGMVALGNEP
                     VRSKSSEVVSRVTSGGSGYSLNKSIAYAYLPMELAESGTDLAVEVFGEEVPAEVVSMP
                     LWDPKHERARG"
     gene            9425..9865
                     /locus_tag="VN178_03020"
     CDS             9425..9865
                     /locus_tag="VN178_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS79971.1"
                     /translation="MSIFPTRILLATDGSKEAVLAACTAVDLAEKTGSELHVIHVHRI
                     LPVPYAYRDRFTEPEPPGGEARALLNRQAREIEKAGGKVVGTYLREGDPDVEIVAVGE
                     EIGAGLIVMGSRGRGGVRRALMGSVSDAVVRHAHCPVMVVRSEH"
     gene            complement(9981..10571)
                     /locus_tag="VN178_03025"
     CDS             complement(9981..10571)
                     /locus_tag="VN178_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79972.1"
                     /translation="MATEVLARQAGTRAKRTGESLEVALRAVLQTEAGRRLERLREGP
                     HRDDRAEQWQPSLPRERAEERQQARIREERRRLREEERGRARKAAWESFMSQERRELE
                     LRKDGQLAELLGEALAGEPPAALRRLAREDQRQAEDGLVALTSNGKLFYKLVDELTEE
                     DMAARIAAARLREAWLKERRDEWLDCGGGSRGEGRL"
BASE COUNT         2046 a         3237 c         3461 g         2037 t
ORIGIN      
        1 tgacgcgagc accacgggcg tcccaggccc caggacgtcc ttccagagcg acgccgtgcc
       61 attcccagct aagaccgtct tcgctggtgg cgtaggccgt gttcatgcga tcctcctcgc
      121 ctggctgatc gaggaggtgg cagcagatcc aggcccgcca tccctggccc gtacgctgcg
      181 ccaacgggtc tttgacgccg gtgaggcgat cgcccgggaa gatggtgcgg gcaccctccg
      241 ctgcgaaccc ttccggttcg tcggcatcga ttgcctcgat ccaccagtgg gcacttccag
      301 ggtcggcgca gcagacataa agacgccagc tccccgtctc ggtacggagg agcgatgggc
      361 gttccatgct cttcgcgccg aactcggact gctccagggt agcgaccgtg gtgaaccgct
      421 cgccgtcatc ggaacgcgcg atcacggtct cagcggcgcc atcgtgaccg tggcggaggc
      481 ggtagccgat gaggaacgtg ccgtcctcgt cgagggcggc gctcgaggcg ccagcccagt
      541 aaccgggtcc gtctcccgga gcaggcacgg cgacaacgga agccgcggat cccggcagcg
      601 ggaaaccgcg caaggagtct tgcggtccag cggtgttgga ggtttcgtct gtcgtcactg
      661 gggactcctc tcgggttgtg gtctctggat gcaccttcgg aaagcaccgg cgaacccata
      721 atagatcggg aatggtggat cagcatcggt tttctcctgg aagccgacca cgagttgtgc
      781 tgaacaggca aactcgagct tacgatcctc tccctaacct tgcaaaaccg ccgtggagag
      841 ataagccccg gaatcttcat gggagctacg gagacatgcg cgcatgcgtt cttcctggaa
      901 ctgcgtacag cggaagttcg actatcgaga acgattcaca ggcgaacttc aggtcccttg
      961 cgcatcgcat gatcggtggg catcataatg ttattgggac tatatgcctt ggggaaggag
     1021 aagtaatgtg tcggatcaga ctacggagcc gagcgtggtc gagggggccc acctgcagaa
     1081 gctcccgaag aggcggcgca tatccacggt ctctttcgtt ggtgccatcg aggtcgggcc
     1141 gttcgtcggt gcatggccag ggctcgacca agatcttgcg tagttcgccg gtgggagtac
     1201 tcgtcatacc ccgcggcgcg gacgtgcgtc cccgggatgc agcgcccgtc actcgaggtt
     1261 tcgaacggga acaataaggt tgcagcctgc gtcggatatc gttgcccccg agaccttggg
     1321 tttcgagagt agcccggaag ggtggtggaa gaccgtcggt gagcggcgac tgcttttcat
     1381 gcgcctgcgc tcgcgcctcg acctggagaa aaccgaacgg cttcagcgct cggtcttcgg
     1441 cgtaaccgac cgcgacctgg cctcatccag catcctcgtc gccgtccacg aaaccggcgg
     1501 ggaggtaatc ggggctttcg atggggaaca gctggtgggc ttcgtatcgg gctggggagg
     1561 ttacgtggac ggccatgcgc gtctggtctc cgacctgatg gcggtggaga gtggataccg
     1621 cggtggcatc gggttcgccc tgaaaggact ccaagccatc attgctttcc aggccggttt
     1681 cgaggagatc gtgtggaccg tcgatccact gcgggccgcg aacgcgcggc tgaacctcga
     1741 gcgcctcggt gcccactcgc gcaaatacct caggaacttg tacggggagc attttgcaga
     1801 aggcctctac ggaggtctgc caagcgatcg cctgctcttg acctggccac tgcgcagcgc
     1861 tcgtgtgagg tcgaggcttc tgggaaggta ccgaccactg gagccaggcg cgctcgccac
     1921 gctaccagag tatgattccg gcacctccga gaggagggtc cgcctcgcca tccccggtga
     1981 cttcgacggt ctcctgtcct ccgatccggc ccgggcgcgc gagtggcgct ataggctaag
     2041 ggataacctc gaggccgcct ttgcctccgg gtacgtcatt acgggcttcg ctggtcggcg
     2101 cggtgaaccc tatggttact atgtgctcga acgcgatttc gtgttcgagt gagagtgagt
     2161 agcgtcacct gattctctgc ggtcgaacgt atgcgaggag gcacagtgcg aatcaccggt
     2221 gtagagatcg tacccgtaaa gctgcccctg ctcgagccgt tcgtcgtctc ttacggcacg
     2281 ttcccggact tggcgaccgt gttggtgcgt ctggagacgg acgagggatt gaccggctgg
     2341 ggagagggga ccccagatcc gcacgtgacc ggcgagacct tcgagggagt cgtcgccacg
     2401 ctacgcctcc tcgccccggc gttgctgggg cacgacccgc tcgatcgttc caccgccatg
     2461 cggctgctga agtcgcgggt cgtgggcgtg ccgactgcga aggccgcgct cgacatcgcg
     2521 ctgcacgacc tcgccggccg cgtcgccggg ctgccggtct ggacgcttct aggcgggcgc
     2581 gctagggaag ccttgtccat ctcccgggtc ataagcctga agtcgcccga ggcgatggcc
     2641 atcgacgcta accagttcgt cgcggcagga ttccgcacgg tcaagctcaa ggtcggagac
     2701 gcccacgaca tcagggggga cgtgcggcgg gttgcggccg tgcgcgaggc cgtcgggccg
     2761 gacatcggga tcaagataga tgtcaacgga gggtggcgca cagccggcgc agcagtcggc
     2821 gccgtaaggg gcatcgccgc ctacgatccc gagtacgtcg agcagcctgt cgaccggcgc
     2881 gacctcgaag gtatggccga ggtgcgccgg ttgggcggcg tgcaggtgat ggccgacgaa
     2941 gctgtactgg acgcccacga cgcgctgcaa gcggttcgac tgcgggcttg cgacctgatc
     3001 aacatcaagt tgatgaagtg cggcggactg ctggccgcgc tggcgctcga cgccgtcgct
     3061 gagaccgcag gagtcggctg ccagataggg accatggtcg aatcttcggt ggcatcggcg
     3121 gccggcctgc acctcgccct ggcgctccac aacgccgcga cggtagagat gggcggaccg
     3181 atcatgttgg ccgaagacgt cggtgatctg cgcgcctact acgagcagca tcgggttacc
     3241 gtacccgccg gtcccggcct cggtgtcgaa ccggaagagg ccgtcctaaa gcgttacgct
     3301 ggccaacgtg actggatcac cgtctaacgc aaccgcgcgt ccgaactctg gtatgtgcga
     3361 ctccctctgc cccaagccaa ggctatataa tgtgcgtatg aagtacgtat tagtacaaac
     3421 atagaccgaa aggcggaagg ggttgtttgc ggacaagggg gagctttctc cgatctaccg
     3481 ggctgtcgcc gaagcactca tcgagcgtat agacgatggt gatctacggc caggaagccg
     3541 atgcccgtca gaacgtcagc tcgcgtccga gtatgggatc tctcgtatga ctgccagggc
     3601 agccgtaaat cttctggtgc agcgcggcta tgtcgcacga aagaatggat cgggtacctt
     3661 cgttgccagt ccgaagatcg agttggactt atcaacggta gcaggtttta gcgaccgggt
     3721 cctgcgccat ggcatcgtgc ccggagctcg agtgatcgag gctcgtacgg tcagcaacga
     3781 ggagctggac gccagtgtgg cgacggccct ggagatcacc ggagaagagc tggtgcacgt
     3841 gcttgtccgc gtgcgtaccg gcaataacga gctcctggct ctcgagagat cctactttcc
     3901 cgcccgccat tgcccggatc tgctcgacaa cgaactgacc ggttcgatat acgaactcct
     3961 gcagactaga tgtaacctcg aactcgcaca cctgcgtcag aagctcgaag tgacccaact
     4021 cgacgcatcg gccgccgaag cgctcgctac ccatccggac gccccggcgc tccgtattac
     4081 ccgtactacc tgggatgccc atgggcgacc gatcgagttc gccagagatc tgtatcgcgg
     4141 ggacaggctg ctgttcgtca ccgaggcccc tgcatcctga tggtttgaac ccgcccttct
     4201 acagcggggc gtagcactcc acgtcggcta cctgcgcaag aaccgtacct cggtgaagca
     4261 tctcataaag aattgacacg atgagcgcat ctgtgtacgc tcatctatac cacaacggta
     4321 tagaccactt ccgtaggtca gggggaaggg ggtggtgttg atgacgatga gcgcttcgag
     4381 ggcgctcgga catacgcaaa gcgtgagtgg ttgactcggt tatgagtccg gggacgccgc
     4441 aatcgtcgct gcaagtggaa atgtgaagat ctgcgaggtg tctctcgtga gagagggcga
     4501 gacgtttgaa gtcgtcgggg tgaggtggag atgactttgg aaacttcgga tccggccctg
     4561 aaagaggtcg tgaactcgat gccggcatgg gcgagcgcgg acggtgtgga agcggagtac
     4621 atctccggcg gtatcaccaa ccgcaattac tgcgtacggg tggacgggga acctttcttc
     4681 gtccgtctgg cgggagaaga aacggaggca ttggggataa accgggaaaa cgagcgctcg
     4741 gcgatagagg cggctgcggc ggccgggatg ggcccggaag tcgtggcttt cctgcccgaa
     4801 cacggttgcc tcatcactga atggatagag ggcgcacccg tgccaccgga agatctgcgg
     4861 cagcggacga tgctagatcg gctggttcgc tccataaggg cgttccatgg ctgctcggag
     4921 attccgggaa cgttctcacc tttcagagtg gtggaggtgt atcgggaatc tgccgagagc
     4981 aggggcgtcg aggtaccgga gatctactgg tggctcttgg agcgggcgcg agaagtcgag
     5041 gaggccttcc agcaagaacc taccccttca cgtccgtgcc acaacgatct gctcaacgca
     5101 aacttcttgc tctgcgacga ccgggtaatg atcgtggact acgagtatgc gggcatgggc
     5161 gacgtgttct tcgatctggg caacctctcc gtgaacaacg atttcgatga ggacgccgat
     5221 gtagcgctac tggagctcta ttttggcgcc gttactcctt cgcgggtagc aaggctgaag
     5281 ctcatgcggc ttatgtcgga ttttcgtgag gcaatgtggg gcgtaatgca gcaggctatc
     5341 tccacactgg acttcgacta cgaagactac gctaaccagc acttcgcgag atgcagagag
     5401 cacgccgagg acgaacggca cccgatgtgg ctacgcgacg ctgctaagaa aacttgagca
     5461 caggaagacg cccgttgttg ctgacggccg gtttggaggg agcacacagc acccccgaac
     5521 gccagagtat ccggcagcac tcggctagag cgttacgcag ctaaggaggg agatcccatg
     5581 gcgataatcg gggacgtagt tgtatacatc ataatggctt tcgtggttgc tggcgccatc
     5641 gcggctatcc gcgacgatga gcatggaatg ggcaaagaat tcaaggaagg gttgcacgcc
     5701 ataggaccca ttttcatccc ggtggctggc ataatggctt cgatcccgta cctctcgcag
     5761 tttatcaagg ccgtggttgg cccggtcttc ggtctggtgg gggcgagccc ggcgatggcc
     5821 tcgacgaccg taatagctgt ggatatgggc ggttaccagc ttgcggaggc cacggccaac
     5881 agcacggcga actggatcat ggcggcggta gtcggctata tggccggggc aaccatagtc
     5941 ttcagcatcc ctgtgggact ggctatgttg gaccagaggg accacaagta catggccctc
     6001 gggatcatgt ctggcattct taccgtgccg ctgggcgtcc ttattaccac cagcatcctg
     6061 tcgattacca acgggcaggt gagggacgag gtctccacca ccaccaactc ggattacgcc
     6121 ctcaacgatc tcacctttgg catgatattc gcgaacctgt ttcccttggc catcatcatg
     6181 attgccatag cgctcgggtt gtacttctat gccaacgcga tgatcaaggg cttcatgtgg
     6241 ttcggtaagg ccatggatac cggcatcaag ctggtcctgg ccttctccat cgtggagtac
     6301 ttcaccgggt tcttctccct ggtcttcggt agctggggat tcgacccgat catcgcggat
     6361 gccgaagatc agttccgtgc tctggagatc gccggataca tcggcatcac actggcggga
     6421 gcattcccaa tggtctacgt gatcaggacg tacctcgcca agcccctggg tgcggtggga
     6481 cgcaggttcg ggatcagcgc cgaggggagc gccgggatcc tggccgctgc cgccaacatc
     6541 ctggctcttt atcatctgat ccggcgcata ccaccgaagg acaaggttct gtgcatagcc
     6601 ttcgcagtct gttctgcgtt cctcctgggg gaccatctgg cgttcaccgc gaacttccag
     6661 cccaacatga tactgccgct tatgctgggc aaggtcggag caggaataat cgccattgcg
     6721 ctggcctact ggatcgccgt acccacggca ctggagctag aacgacgaga tcgcgaagaa
     6781 ggcgtcatag gagaagacga atatccatct gtcggagcgt caagggccgg cgaagaggag
     6841 agcgtcgagg aagtcgctac cggcgggcgc gaggagaggg agtaaactgc gcgttcctcg
     6901 caagggggat gctcgtaaag ccgcgtgatt cacgacaaga gtccgagata cagccgggag
     6961 gtgcaacggg tgaacgagga ggcgcaggca gtgatcatcg gtggtggggt ggggggcgca
     7021 agcatcgctt accacctgac cgggataggc tggcaagacg tggtgctcgt cgagaagagc
     7081 gagctcacgc atggcactac cttccactcg gcgggtctgg tcggacaact caggcccacc
     7141 gagacgctga ccagaatgaa catgcacagc gtggaactct atcgtcggtt gcagaaggag
     7201 accggcgccg accccggttg gaaggaagtc ggaagcctca ggctggtctc ctcgcgcgac
     7261 cgaatggagg agctcaccac gctcgtcggc cgtgcgaaaa cgttcggact acctctggaa
     7321 ctcatcggca ccgaggaggc gctcgaactc ttccctctct tcgatccgag gggtgtgttg
     7381 ggcgccgctt acgatccgac agacgggtac atcgacccga gcgccctcac ccaggccctg
     7441 gccgaaggag ctcgtttgag aggagccaag atccacacca acgtgcgggt caccgggatc
     7501 accgtcgata acgggcgcgt aaaggaggtt gccacagatc acggcgtcat caggactgag
     7561 gtggtaatca acgcaacggg catctgggca ccagagatcg gcaaactcgt cggggtgaac
     7621 gtgccgctta tcccgttcca gcaccagtac gtgcacgtga agcccgaaga gcctatctcg
     7681 ccggacttcc caaccatgcg tgatcccgac gacctggtct actttcgccc ccacgagggc
     7741 gacataatga ccggaggcta cggcaggcac cctgccccct tcgcgctcga cggcgtccct
     7801 gcggacttcg gccacaagct cctcgagcct gactggggca ggttctcccc gctgttcgaa
     7861 gccgcgtgcg cccgggttcc cgctatccgg gatggcgaag tggtccagct cactaacggg
     7921 cccgagtcct tcaccccgga cggcgagttt attctaggcg agtcgcacgt gcgaggattc
     7981 ttcgtggcgg cggggttcaa cgcccatggg atagccggcg ccggaggcat gggccagatc
     8041 atggccgagt ggatagtcga gggcgaaccg agcatggacg tctggcagat ggacatccgc
     8101 cggttcggag agcattaccg cagccgccga tacaccctgg agcggtcatc cgaggcgctc
     8161 tccacctact acgacatcca cttccccaac gaagaatcgc ggagcgcgcg cgggctccgg
     8221 ctctcccccg cctacgagag gttacgggcc ctggatgccg agttcgggga gaaggccggt
     8281 tgggaacggc cgaactactt ccgatccaac gaagatcccg cctacgaacg cctacgtcca
     8341 cggggctggg ccggacggct ctggtccacg gctatcccgg cggagcacct ggccacccgg
     8401 gagcgtgccg ggttgttcga ccagacctcc ttcgcgaaga tcgagatcga ggggcccggg
     8461 gcctgtgcgc tactgcaacg cctttgcgac aacgacgtag acaaaccgcc cggcacagtg
     8521 acctacaccc agatgctcaa ccctcggggg ggcatcgagt gcgacttcac cgtgacccgc
     8581 ctcgaggatg agcgcttccg tatcgtcacc ggaacggcct tcggaatcca cgacatggac
     8641 tggatccgcc gacaccaacc ggacgacggc tccgtccaga tcagggacgt aacgtccagt
     8701 ctcgcttgca tcggccttca gggtccgcga gcgcgggaca ttctttcctc ggtatgccag
     8761 gacgacctct ctaacgaggc cttcccgtat atgagggcgc gcgagataac cgtccaggag
     8821 gtaccgtgtc ttgcgctacg cgtgacgtac gtgggggaat tgggctggga gctgtacccg
     8881 cccatggagt tcggcacccg cttgtgggac gtgctcttcg aggccgggcg accccacggt
     8941 ctcgtaccgg ccggctaccg ggcgatcgag tccatgcgtc tggagggggg ttttctcgcg
     9001 tgggcggccg acatcactcc tgaggacaac ccttacgagg cggggctcgg tttcgccgtc
     9061 aaactcgaca agtcgatgcc tttcatcggc aaggaagccc tggagaagat aaaggctcag
     9121 ggcacacagc gtcggctgcg ctgtctcgta ctcgccgacc aaggcatggt cgccctcggc
     9181 aacgaacccg tcagatcgaa gtccagcgaa gtcgtctccc gggtgacctc tggcgggagc
     9241 ggttactcgt taaacaagag catagcgtac gcctaccttc ccatggagct cgccgagtcc
     9301 ggtacggatc tggcggtcga ggtgttcggc gaggaggtac ccgccgaggt ggtttcgatg
     9361 ccgctctggg atcccaagca cgaacgggct agggggtagg aggcatgatg agaggtggga
     9421 gtcgatgagc attttcccga caaggatcct cctagccacc gatggttcga aggaggcggt
     9481 cttggccgcc tgcactgcgg tggatctggc cgaaaaaacc ggctcggaac tgcacgtaat
     9541 ccacgttcac cgcatcttgc cagtccctta cgcttaccgt gatcgcttta cagaaccaga
     9601 accgcctgga ggagaggcca gagcgctcct caatagacag gccagagaga tcgaaaaagc
     9661 cggggggaaa gttgtgggga cttacctcag ggaaggtgac cccgacgtag agattgtggc
     9721 tgtgggagaa gaaataggcg cgggactgat agtcatgggc agccgcggcc gcggtggagt
     9781 tcgacgagcc cttatgggaa gcgtctccga tgccgtagtt cgccacgccc actgccccgt
     9841 gatggtggta cgcagtgaac actaacgggc ggcaggcata acctccttct cgtgtacgaa
     9901 tgaagttgaa ctgcggtttg cgatcctgaa cttgcacgct cgttcatcgc gaactggaga
     9961 atgcagagga gtatgttttg ctacaggcgg ccttcccctc gggagcctcc cccgcaatcg
    10021 agccactcgt cccgccgctc cttgagccac gcctcccgca acctggcagc tgcgatcctg
    10081 gccgccatgt cctcttctgt gagctcgtcc acgagtttgt agaacaattt gccgttgctc
    10141 gttagcgcca cgagcccatc ttcggcctgt ctctggtctt cgcgggccaa ccgccgcaag
    10201 gccgccggtg gctctcccgc caacgcttcg cccagcaact cggccagctg gccgtccttg
    10261 cgcagctcga gctcccgccg ttcctgactc atgaacgatt cccaggcagc tttgcgggct
    10321 cggccacgct cttcttcccg gagccggcga cgttcttctc gaattcgggc ttgttggcgc
    10381 tcctcggccc gctcccgcgg taggctcggc tgccactgct ccgccctatc gtcgcgatgg
    10441 ggaccctcgc gcagccgctc gaggcgccgt ccggcttcgg tttggagaac ggctctcaag
    10501 gctacttcca acgattcgcc ggtccgtttg gcccgggtgc ccgcctggcg cgccaggacc
    10561 tcggttgcca tctcggaaac actttgatcc tgttgcataa gctagccttc ctagaaatac
    10621 tcgggccgac gaagcccctc ctacagggag tcggcgatcg cgtgttggag aaggagaggc
    10681 tcctatcggt tggcttcccc atctggcatc tcggtggaca cctcctcgct cgacacctcg
    10741 cgcgtagcgt ctcctacggc ctcctcgtag aggcgctcgg c
//
LOCUS       DATMVF010000069        15286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_51778, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000069 DATMVF010000000
VERSION     DATMVF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51778"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VN178_03030"
     CDS             complement(<1..468)
                     /locus_tag="VN178_03030"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HWS79973.1"
                     /translation="MTANVTKLEENKVRLDVEVTPETVRRGVEAKIRELGQQVRVPGF
                     RPGKAPRRVIENRLGRDFIYMEALQEQLPGWYSQAVVETDVRPIDRPEIHFDDPLDEE
                     EGFKFSATVEVRPEAVLGDYKGVEVPRREVEISDEDVEDRIEEMRAQFATLAAV"
     gene            complement(511..591)
                     /locus_tag="VN178_03035"
     tRNA            complement(511..591)
                     /locus_tag="VN178_03035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Leu,seq:tag)
     gene            complement(849..1829)
                     /locus_tag="VN178_03040"
     CDS             complement(849..1829)
                     /locus_tag="VN178_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWS79974.1"
                     /translation="MLDSLWMREFQAYTRARQGTRRSGWRIWALPFVLIALFVGIEFV
                     AGPVSIQRLTSASSPDPGKVEASPSDWVERMFGGRPVFASGPLNVLVLGVDTRPDSQE
                     MGSRTDTIMLVQVVPKTGNVKLLSIPRDLLVEVQPGEWGKINAAYNYGGVNETIDALE
                     NYSGVPVDHYAVVDFEGFERVIDAMGGVRVDVGDGQFPEKWRMGEDVQRLNGHKALIF
                     ARYRGTPGADLDRMQRQRELVGALRSEALRWHTVKTLPEIMAVMNENIQTDLDLDGAI
                     TLGQVLIRKGRHAEMTSQQLNGIPETLPNGEQVLIPDEDANYAILDEFRQ"
     gene            complement(2057..2890)
                     /locus_tag="VN178_03045"
     CDS             complement(2057..2890)
                     /locus_tag="VN178_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS79975.1"
                     /translation="MEQVQDLFSLAGKVAVVTGATGVLGSEMARSLAQSGARVAVLGR
                     REERANRVAQEIEVAGGQSLPLPADVLDTGQLRSARDALLERWGRVDILVNAAGGNVP
                     GATLSDKVGVFELPEKALRQVMDLNFLGTLLPCQIFGAAMVEGTGEPEGCIVNISSMA
                     ASKTLTNVVGYSAAKAAVDNLTRWLAVELARSYGPGLRVNAIAPGFFIGEQNRNLLLD
                     EDGSPSKRGQTIIEHTPAGRFGEPHELAGTLIWLCSPASAFVNGIVVPVDGGFSAFSG
                     V"
     gene            complement(2902..4344)
                     /gene="uxaC"
                     /locus_tag="VN178_03050"
     CDS             complement(2902..4344)
                     /gene="uxaC"
                     /locus_tag="VN178_03050"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HWS79976.1"
                     /translation="MSKPWTLDPDRLFDPNPARRTLARSLYKEVEGLPIVGPHGHVPP
                     ALLADPDATLGTPAELFIIPDHYVFRMLYSQGVPMEDLGVPTVDGTPVETDHRHVWQR
                     FCENFHLFRATPTGLWLSAELTGVFGVTERPSAENAQNIYDHLQEKLASPEYAPRALF
                     EKFGVEILCTTDAATDTLEHHQALRAEGWGGRVRPTFRPDAVTDISTPGWRENIDLLS
                     QASGIDIVDYRSFLEALQERRSYFKEMGAVATDHSATTPHTERLSETEAEDIFARARS
                     GESSCEDVARFTANALVEMAAMSVEDGLVMQLHAGSLRNHNRVVHRRFGPDAGADIPI
                     AADWTRGLQALLDEWGNDPRFRLVVFGLDEAAYSRELAPLAGHYPALLLGAPWWFFDS
                     PLGMRRYLDAVVETAGLYNTAGFNDDTRAFASIPSRHDVWRRTSSDWLAGMVLTGVVD
                     EEEAPDMAREFACGLAKRAYGFQREPVGRA"
     gene            complement(4341..5849)
                     /locus_tag="VN178_03055"
     CDS             complement(4341..5849)
                     /locus_tag="VN178_03055"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HWS79977.1"
                     /translation="MGLTGNTPTALLALNGLGVRPNSAVSVEGMEVGLVEEDRKIAVL
                     VPGGSRVFSDFEGESSAVGDRTLVLGPTNAANLDVLRGLLPWLRPRPLGTRTSAGFGD
                     RLGPATPGHVRALRAAGGGLAPIFAQQSIREMERTGRSPRQVLDDATWGVFAEGWQEG
                     FGADADHLKTAEDVDACVSAGYTFFTFDPGEYVEDGAEAASASDLRAALDNLPWQDLE
                     DAPGDLRRRYLGETFEVEGHGVPLDDASLVRAAVKYGAAVAHVARLHRHLQVAMGEGD
                     FEVEVSVDETESPTTHAQHVYIATELQRLGVRWVSLAPRYVGRFEKGVDYIGDVAEFE
                     EDIEVHAAISRTVSPDGPYKLSLHSGSDKFSVYPAFARQTRGLAHLKTAGTSYLEALR
                     TAAALDPGLFREIYELSRARYEEDRASYHVCASLEQAAKPEAVPDEELPALLERFDER
                     QILHVTFGSVLKEAHFRDHLLGLLREHPENYAGGLEKHFLRHLEPFANGGGQ"
     gene            complement(5840..6667)
                     /locus_tag="VN178_03060"
     CDS             complement(5840..6667)
                     /locus_tag="VN178_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWS79978.1"
                     /translation="MWTLTGFADEISADLEEQLETLAQESMGYMELRSVWNTNVLDLT
                     DDELERVKSSTAQRGIRISSIGSPIGKVPVTNPFGPHLERFRRALYAADVMGAPYIRI
                     FSFFIPEGEEPEQYREEVIDRLGIMAGEAEDSGVTLLHENEKEIYGDIPSRCLDILAG
                     VGSPALRAAWDAANFVQCGISPYKEGYASLRPYIEYVHVKDALSGSDTVVPAGEGDGQ
                     WPETLSALRRSGFDGFFSLEPHLASAGTYSGFSGPELFRKATGAFKELLRSQGIEWA"
     gene            complement(6722..7810)
                     /locus_tag="VN178_03065"
     CDS             complement(6722..7810)
                     /locus_tag="VN178_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS79979.1"
                     /translation="MSSEELRFGILGCGVIGPHHAKSIAGLKSAELVAVADVVPERAE
                     ELAGEYGCAHYASLEEMLAGVDLDAVCVCTPSGMHADGAITALEAGKHVVIEKPVDVT
                     LEAADRLIEVQGATGGRVAVVSQHRFDAATQAVHEALARGEFGRLTSGSADVRWWRSQ
                     SYYDSGGWRGTWELDGGGVLINQAIHSIDLLQWLMGRVVEVTAYTGLIAHERIEVEDT
                     AVAIVKFANGAFGTIVATTAAYPGLTTRIAVHGDRGSAIIDDDELTYFHAAREGQEGD
                     AYGAGGGENQAELVMQPYDDAPSGPGAGADPGSLSMAHRDQIHDFIEAIREDREPLVN
                     LQEGRRPLAVVQGIYESARTGGPVRIEE"
     gene            8201..9535
                     /locus_tag="VN178_03070"
     CDS             8201..9535
                     /locus_tag="VN178_03070"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79980.1"
                     /translation="MEARGGPRIAFVGGGSYQWGPKIIQDVALNEELRGGTLVLHDVN
                     GEALQDMYEWGTRALDVAKADLQLEKTQRLEEALSGANFVVLSISTGGLDATALDLEI
                     PARYGVVQSVGDTVGPGGLFRGLRNIPVVVEIARSMEEYCPHAVLLNLTNPLTVLTRA
                     VTKATSIKAIGLCHELFSTLGMLSKMFHAPEEGINVRVAGVNHFIWVTGVSVHGRDVT
                     GEAFRRISGGEAREISLSDDADDTDPFTNTWGFRTELCRLYGYLPAAGDRHVCEFLPG
                     YLPDEKERERLDLRVTAVDVRRERLAADRESVRLMVRGEEPIPTGRSREEISDIMAAM
                     WTGVDSVNIVNLPNAGQVRDVPLGAVMETYGALNGSGASGIVFGELPPPVAALVHPHV
                     FNQEAIVQAGLTGDKNLAFCAFLNDPLVDRGPDARTLFEEMFEAQREYLPQF"
     gene            complement(9579..12134)
                     /locus_tag="VN178_03075"
                     /pseudo
     CDS             complement(9579..12134)
                     /locus_tag="VN178_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            complement(12131..12535)
                     /locus_tag="VN178_03080"
     CDS             complement(12131..12535)
                     /locus_tag="VN178_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79981.1"
                     /translation="MPTALKIRDETTLSLGGDDERDFILDVPDEWITVRQLIRARVYR
                     EVHDYNLDQPEYFHGLIQPSDAERSLNGFKMRKRRSIDPEKQFELAVRAFYTNGFILL
                     VDDRQVDELEEEIEVRPDTTVTFLKLVPLVGG"
     gene            complement(12779..14155)
                     /locus_tag="VN178_03085"
     CDS             complement(12779..14155)
                     /locus_tag="VN178_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWS79982.1"
                     /translation="MRTTSREAIATAEVPPGPRGNVVLGSIGDLYRDRLRFVLDVART
                     YGDVAQYRVAHVRMYQVNDPAGVGRLLHDNHRNYSKDVATFGTLRLILGNGLFTSDGD
                     FWRRQRRLAQPAFHRRRVAAFGDLMTDATLAMLKRWGPSVARGQPLDVSTEFMHLTLE
                     VATRALFSTSVEQDVDAIGHAITTLLNEVTFRFTFPFYPPLKVPTPRNRRFLAARATL
                     DGVIYGIVAERRRRPGEHDDLLALLMEARDEETGEGMSDRQLRDEVITLFVAGHETTA
                     NALTWASYLLSTHVAVAHKLRAEVDEVLQGRIPTAADLPNLPYTRMVVDETLRLYPPA
                     WITNRRAIQDDTICGYRIPADAIVSISPYVTHRDPTLWENPEGFDPERFAPERSARRP
                     HYAYFPFGGGPRQCIGKGFALMEATLVLALLAQHYDLHLVPGRRVESVAMATLRPSNG
                     MWMTAHPR"
     gene            14462..15253
                     /locus_tag="VN178_03090"
     CDS             14462..15253
                     /locus_tag="VN178_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWS79983.1"
                     /translation="METQVLFFVAAVVAGLINSLAGGGGLITFPLLALVVPPVVADAT
                     SAFALLPAYPSAVWRTRSRLREVRRLWLWLLLVTSALGGLVGALLLVWTGERNLQFLV
                     PWLVFGGTVLFVLEPWLSRRSGDAHTDRGLATALWPLAAVVVFVVAVYGGYFGAGIGI
                     LMISALSMLRMGDVHRVVPFKNLLVGCLRAVAVFVLVIYGEIAWRYGFPMAVGGLIGG
                     YLGGVLTGRVNRTILRSVVVTIGFGLAAYYFWTLYAPAGLPFGGE"
BASE COUNT         2513 a         4850 c         4874 g         3049 t
ORIGIN      
        1 gacggcggcc agggtggcga actgggcccg catctcctcg atcctgtcct cgacgtcttc
       61 gtcggagatc tccacctcgc gccgtggaac ttcgacgccc ttgtagtctc cgagtacggc
      121 ctcgggccgc acctccacgg tggccgagaa cttgaagccc tcctcctcgt ccagggggtc
      181 gtcgaagtgg atctccggcc ggtctatggg gcgtacgtcg gtctcgacga cggcctgcga
      241 gtaccagcct gggagttgct cctggagggc ctccatgtag atgaagtcac gcccgagccg
      301 gttctcgatc acccgccgcg gcgccttgcc agggcggaaa ccggggaccc tgacctgctg
      361 gccgagttcc ctgatcttcg cctcgacacc ccgcctgacc gtctccgggg tcacctcgac
      421 gtcgagcctg accttgttct cctcgagttt cgttacgtta gccgtcatac cctccagttc
      481 ctcttgtgcg gcccgacgcg ggcggcgatg tgcgagaggc gggatttgaa cccgcacgcc
      541 gtgaggcact agatcctaaa tctagcgtgt ctaccattcc accactctcg ctcaccgtag
      601 gacctgcgcc ggattatagc agccacccaa tcgcttcccg cccgcatttc gcccctttac
      661 gctacaggcg caagcgttgc ggtcagctga aagctgatcg cgcatgtcag cacgcgccct
      721 gtcgggcgct tgtcagcatt tcagcacgct cgagccttcg gctttcgctt gtcagcaagg
      781 acttacacct gcgaaagatg aagtgctgac aagcctgcga agcaggcgtg ctgactcgct
      841 gaagtgcttt actggcggaa ctcgtcgagg atagcgtagt tggcgtcctc gtctggtata
      901 aggacttgct ctccgttggg gagggtctcc gggatgccgt tgagttgctg ggaggtcatc
      961 tcggcatgtc tacccttgcg gatgaggacc tggccgaggg ttattgctcc gtcgagatcc
     1021 aggtcggtct ggatgttctc gttcatgacc gccatgatct cgggcagggt cttgacggtg
     1081 tgccacctca gggcttcgct gcggagggct cccacgagct cccgctggcg ctgcatgcgg
     1141 tcgaggtcgg caccgggggt gcctctgtac ctggcgaaga tcagggcttt gtggccgttg
     1201 agccgctgca cgtcttcgcc catgcgccac ttctcgggga actggccgtc tcctacatcg
     1261 accctgaccc cgcccatcgc gtcgatgacc ctctcgaaac cctcgaaatc cacgacggcg
     1321 taatggtcta cgggtacgcc cgagtagttc tccagcgcgt cgatcgtctc gtttacgccg
     1381 ccgtagttgt aggcggcgtt aatcttgccc cattctcccg gctgtacttc gacgaggaga
     1441 tcccgcggta tagagagcag cttgacgttt cccgtcttcg gcaccacctg cacgagcatg
     1501 atggtatccg tgcgactgcc catctcctgg ctgtcgggcc tcgtgtccac gccgagaacg
     1561 agcacgttga gcggtccgct ggcgaatact ggtctcccac cgaacatcct ctcgacccag
     1621 tcactagggg aggcctcgac cttgccagga tcgggcgaag acgcactggt gagcctctgg
     1681 atcgagaccg ggccggcgac gaactcgatg cccacgaaga gggcgatgag cacgaagggc
     1741 aacgcccaaa tcctccaacc ggaccggcgc gtgccttgac gtgccctggt gtaagcctgg
     1801 aactccctca tccacaggga gtctagcacg ctccgccctt cggcctccgc tcgtcagcta
     1861 gtcagcacgc ctgcttcgca ggcttgtcag catttcagcc agtcgttcca gtgcgtcgcc
     1921 acctgcgtcg aacctgaccg tcccgagcag cggttcctcg tgcgcggcag ggcgacgctg
     1981 gaaaggcact agctgaagtg ctgacaagcg agggcctgca aaggcccgaa gcgtgctgac
     2041 tagctgacat gctgacctac actccgctga atgcggagaa gcctccgtct acgggcacga
     2101 cgatcccgtt aacgaaggcg gaggccgggc tgcagagcca gatcagggtc cctgccaact
     2161 cgtggggttc accgaagcgc cccgcagggg tgtgctctat gatggtctgt cccctctttg
     2221 agggactacc gtcctcgtcg agcaacaggt tcctgttctg ctcccctatg aagaagcctg
     2281 gggctatggc gttgaccctg aggccagggc cgtagctgcg cgcgagctct acggcgagcc
     2341 agcgggtgag gttgtcgacg gcggccttgg cggccgagta accgaccacg ttggtcaggg
     2401 tcttggaagc ggccatggaa gagatgttca cgatacagcc ctcgggttcc ccggtgcctt
     2461 cgaccatggc agccccgaag atctggcagg ggagcagggt tcccaggaag ttgaggtcca
     2521 tcacctgccg cagggccttc tcaggcaact cgaagacacc gactttgtcg ctgagcgtgg
     2581 ccccgggtac gttcccgccg gcggcgttta cgagtatatc cacccggccc cagcgctcga
     2641 gcagggcgtc cctggcgctt ctgagctggc cggtatcgag cacgtcggcg ggtaggggga
     2701 gcgactgccc accggccact tctatctctt gcgccacccg gttggccctc tcctcgcgac
     2761 ggccgagcac tgccaccctg gcgccactct gtgcaaggga gcgggccatc tcggagccga
     2821 gtacacctgt cgcgccggtg acgacggcga ccttccccgc gagggagaag agatcctgca
     2881 cctgttccac ctttctcctc cttacgccct acccacgggc tctctttgga acccgtaggc
     2941 cctcttggcg agcccgcagg cgaattcgcg cgccatgtca ggtgcttctt cttcgtcgac
     3001 gacgccggtc aggaccatgc ctgccaacca gtcgctactt gtgcgcctcc acacgtcgtg
     3061 gcgggaggga atgctggcga acgcgcgggt gtcgtcgttg aagcccgccg tattgtagag
     3121 tcctgccgtc tccactaccg cgtcgaggta gcgccgcatg ccgagcgggc tgtcgaagaa
     3181 ccaccacggc gccccgagga gcagggccgg atagtgcccg gcgagcggcg ccagctccct
     3241 ggagtacgcg gcctcgtcga ggccgaatac tacgagccgg aagcgcgggt cgttccccca
     3301 ctcgtcgagg agggcctgca ggccccgcgt ccagtcggcc gctatgggaa tgtcagcccc
     3361 ggcgtcagga ccgaaacgcc tgtgcacgac cctgttgtgg ttgcggagac tgcccgcgtg
     3421 taactgcatc accagcccgt cctcgacgct catcgcggcc atctcgacga gggcgttggc
     3481 cgtgaacctg gcgacgtcct cacagctgga ctctcccgag cgtgcacggg cgaagatatc
     3541 ctccgcttct gtctctgaga gacgctcggt gtgcggggtg gtcgcgctgt ggtccgttgc
     3601 gaccgccccc atctccttga agtaggaccg ccgctcctga agggcctcca aaaaggagcg
     3661 gtagtcgacg atgtcgatgc cggaggcctg ggagaggagg tctatgttct ctcgccagcc
     3721 cggtgtagag atgtctgtca cggcgtcggg cctgaaagtt ggcctcaccc tgccgcccca
     3781 accctcggcg cgcaacgcct gatgatgctc cagcgtgtca gtggcggcgt ccgtcgtgca
     3841 caggatctct acgccgaact tttcgaagag ggcgcgcggc gcgtactcgg gactggccag
     3901 cttttcctgg agatggtcgt agatgttctg cgcgttctct gccgacggtc tctcggtgac
     3961 gccgaagacg cctgtgagct cggcggagag ccagaggccg gtcggagtag cgcggaagag
     4021 gtggaagttc tcgcagaagc gctgccagac gtgcctgtgg tccgtctcga cgggggtgcc
     4081 gtcgaccgtg ggaacaccca ggtcttccat cgggactccc tggctgtaga gcatcctgaa
     4141 gacgtagtgg tcgggaatga tgaacagttc ggccggcgta ccgagggtgg cgtccgggtc
     4201 cgcgagcagc gccgggggca cgtggccgtg cggcccgact atcgggagac cctcgacttc
     4261 tttgtagagg ctcctggcca acgtacgccg ggccgggttc gggtcgaaga gcctgtcggg
     4321 gtcgagcgtc cagggcttgc tcattggcct cctccgttcg caaagggctc gaggtgtcgc
     4381 aagaaatgct tttcgagacc gccggcgtag ttttcgggat gctcgcgcag cagccccagg
     4441 agatggtcgc ggaagtgtgc ctccttgagc accgacccga aggtaacgtg caggatctga
     4501 cgctcgtcga agcgttcgag cagagcggga agctcctcat ccggcacggc ctctggcttg
     4561 gcggcctgct ccagactcgc gcagacgtgg tagctcgccc tgtcctcctc gtagcgcgcg
     4621 cgggagagtt cgtagatctc gcggaacagg ccggggtcga gcgctgcagc ggtgcgcagg
     4681 gcctccaggt agctcgtgcc cgccgtcttg aggtgggcga gtcccctggt ctgccttgcg
     4741 aaggctgggt agacggagaa tttgtccgag ccggagtgca ggctaagctt gtacggcccg
     4801 tcgggcgaga cggtgcggga gatggcggcg tggacctcga tgtcctcctc gaattccgcg
     4861 acgtcgccta tgtagtctac gcccttctcg aagcgtccga cgtagcgagg ggccaggctc
     4921 acccacctga ccccgagacg ctgaagctcc gtagcgatgt agacgtgctg ggcgtgggtc
     4981 gtggggctct ctgtctcgtc cacggatacc tcgacctcga agtccccctc ccccatcgca
     5041 acctggaggt ggcggtggag acgcgccacg tgggccacgg cggccccgta cttgacggcc
     5101 gctcgtacca gggaggcatc gtctaagggg accccgtgcc cttcgacttc gaatgtttcg
     5161 ccgagatagc gcctcctcag atcgcccgga gcatcttcga gatcctgcca cggcaggttg
     5221 tccaaagctg cacgcaggtc cgaggcgctg gcggcttcag ccccgtcctc gacgtactcg
     5281 ccgggatcga aagtgaagaa ggtgtaacct gcggagacgc aggcgtccac atcttcggcg
     5341 gtcttgaggt ggtcggcgtc ggccccgaag ccctcctgcc agccttcggc gaagacgccc
     5401 caggttgcgt cgtcgaggac ttgccggggg ctcctgcccg tacgctccat ctctcttatg
     5461 gactgctggg cgaagatggg cgcgagccct cctccggcgg cgcgcagggc cctgacgtgg
     5521 ccgggggtgg cggggccaag cctgtcgccg aagcccgcag aggtacgcgt gccgagcggc
     5581 cgcgggcgca accacgggag caggccgcgc aggacatcca ggttcgcagc gttcgtcggg
     5641 ccgagcacga gcgtacgatc gccgacagcg ctgctctccc cttcgaagtc ggagaacacc
     5701 cgagatcccc cagggacaag gacggcaatc tttcggtcct cttcgaccag gccgacttcc
     5761 atgccctcga cggagacggc cgagtttggg cgtacgccga ggccgttcag ggcgagcagc
     5821 gcggtgggcg tgttcccggt caggcccatt cgatgccctg actgcggagg agctccttga
     5881 aggctccggt ggctttgcga aacaactctg ggccgctgaa acccgagtag gttcccgcgg
     5941 atgccagatg cggttccagc gagaaaaaac cgtcgaagcc ggagcgacgt agcgcagaga
     6001 gggtctcggg ccactgcccg tccccctcac ccgccggaac gaccgtgtcc gagccggaga
     6061 gggcatcctt tacgtgcacg tactcgatat agggccgcaa cgatgcgtat ccttctttgt
     6121 acgggctgat gccacactgc acgaagttgg cggcatccca cgcggcccgt agggcgggcg
     6181 agcctacacc agcgagtatg tccaggcaac ggctcgggat gtcgccgtag atctcctttt
     6241 cgttctcgtg caggagcgtg accccggaat cctccgcctc gccggccatg atccccaacc
     6301 ggtcgatcac ttcttcccga tattgctcag gctcctcacc ttcgggaata aagaacgaaa
     6361 agatgcgaat gtaaggcgca cccatgacgt cggcggcgta cagcgcgcgc ctgaaacgct
     6421 ccaggtgcgg cccgaacggg tttgtcacgg ggactttacc gatcggcgag ccgatggaag
     6481 atatcctgat accgcgctgt gcggtcgagg actttaccct ctccagttca tcatcggtca
     6541 ggtcgagcac gttggtgttc catacgctgc gcaactccat gtagcccatg gactcctgcg
     6601 cgagtgtctc cagttgctcc tccagatcgg cggagatctc atctgcgaaa cccgtcaagg
     6661 tccacacgtg tcataacccc tttcgtggga gcggttccct cgcggtccgc cccggctcga
     6721 tctattcctc gatacgtacg ggcccgccgg tacgggcaga ctcgtagatc ccctgtacta
     6781 cggcgagcgg ccggcgtcct tcctggaggt ttaccagcgg ctcgcggtcc tctcttatgg
     6841 cctcgatgaa gtcgtgtatc tggtcgcgat gcgccatgga gaggctgcca gggtctgcac
     6901 cggcgccggg tccggatggc gcatcgtcgt agggctgcat gaccagttcg gcctggtttt
     6961 cacctccgcc cgcgccgtag gcatctccct cctgtccctc gcgggcggcg tggaagtagg
     7021 tcagttcatc gtcgtcgata atggccgatc cccgatcgcc gtgcacggct atcctggtgg
     7081 ttagtcccgg atatgccgcc gtcgtcgcca ctatggtgcc gaaggcgccg ttcgcgaatt
     7141 tcacgatggc taccgccgtg tcttcgacct ctatcctctc gtgggcgata agccccgtgt
     7201 aggcggtaac ttcgactacc cggcccatca accactgcag gaggtcgatg ctatgtatgg
     7261 cctggttgat caagactccc ccgccatcca actcccaggt tcctcgccag ccgcccgagt
     7321 cgtagtaaga ctgcgacctc caccaccgca cgtcggcgga tcccgaggtc aggcggccga
     7381 actctcctct ggcgagcgcc tcgtgaacgg cctgcgtggc agcgtcgaag cggtgctggc
     7441 ttacgacggc gaccctcccg cccgtggcac cctgtacctc gatcaggcgg tccgccgcct
     7501 ctagcgtcac gtcgacgggc ttctcgatga cgacgtgctt gccggcctcg agcgccgtta
     7561 tggcaccatc ggcgtgcatg ccactggggg tgcagacgca cacggcgtcg agatccacac
     7621 ccgccaacat ctcttcgagg ctagcgtaat gcgcgcaccc gtactcaccc gccagctcct
     7681 cggctcgttc ggggacgaca tccgccactg ccacgagctc cgcgctcttt aggccggcta
     7741 tggacttcgc gtggtgcgga ccgatcacgc cgcatcccaa tataccgaaa cgcagttctt
     7801 cggagctcat gtatcttctc cccctgtgtt cgttccagac gatcgatatg tgcggcaagc
     7861 tctggcggac gatgagctcg ggcctgagca agatttcgct cccccgcagc cacccatact
     7921 atcgaggagc atcctggcag gtctcgcgac gatggcgtac ttgggcacgc tgaaggtggt
     7981 taacgcaggc gagagaagac cggcgaccgt tccgtcgtca caccgaccca ccgcgatgtc
     8041 gtcaggaaca ttcaaaccct acccggcact gaccctcaac gcttggacgg cgatgttgtg
     8101 cctgttcacc ctctcccgag aaccgacccc gctctttggc gctaacgcgc acggtatcat
     8161 agctccttgc acgtgtcagc gggagtagag ggaggaagac gtggaagcgc gtggcggacc
     8221 caggatagcg tttgtgggtg gggggagcta ccagtggggg cccaagatca tccaggacgt
     8281 cgcattgaac gaggagttgc gcgggggcac tctggtcctg cacgacgtaa acggtgaagc
     8341 gctccaggac atgtacgagt ggggaacgag ggccctggat gtggcgaagg cggatctgca
     8401 gttggagaag acgcagcggc tggaagaggc cctcagcggt gcgaacttcg tggtcttgag
     8461 catctcgacc gggggactcg atgctacggc gctcgacctg gagatccccg cccgctacgg
     8521 ggtggtgcag agcgtcggcg acaccgtagg tccgggtggt ctcttccgcg gcctacggaa
     8581 catccctgtg gtcgtggaga tcgcccgctc tatggaggag tattgccccc atgccgtact
     8641 gctgaacctc accaacccgc taacggtact caccagggcc gtcacgaaag caacatccat
     8701 caaggccatc ggtctctgcc acgaactctt cagcaccctc ggcatgctct ccaaaatgtt
     8761 ccacgctccc gaggagggca tcaacgtcag ggtggcaggg gtcaaccact ttatctgggt
     8821 caccggcgtc tcggtgcacg gtcgcgacgt cactggcgag gcgttccgca ggatctccgg
     8881 cggcgaggcg cgtgagatct cactctccga tgatgccgat gacaccgacc cgtttacgaa
     8941 cacgtggggt ttccgcaccg aattgtgccg gctctacggc tatctgcctg cggctggcga
     9001 ccggcacgtg tgcgagttcc tccctggtta cctcccagac gagaaagagc gggagcggct
     9061 cgacctcagg gtgactgcgg tagacgtcag aagggaacgg ctcgccgcag acagggagag
     9121 cgtgcggctt atggtcaggg gcgaggagcc catcccgacc gggcgctctc gggaggagat
     9181 ctccgacatc atggcggcca tgtggaccgg cgtagactcc gtgaacatcg tcaacctgcc
     9241 gaacgccggc caggtcagag acgtgccact aggagcagtc atggagacct acggcgcgct
     9301 caacggctcg ggggcttccg gcatcgtctt cggggagctt cccccacctg tagccgcgct
     9361 cgtccatcca cacgtgttca accaggaggc catcgtgcag gccggtctta caggagataa
     9421 gaacctggct ttctgcgcct tcttgaacga tcccctcgtt gatcgcgggc ccgatgcgag
     9481 aacattgttc gaggagatgt tcgaggctca gagggagtat ctgccacagt tctagcattt
     9541 cagcacgctc cagcctttgg ctgtcgctcg tcagcaattc agctcgagat ttggtgcagg
     9601 attgtggggt cttttatctc tttgtcttct gccagcagga aggctttgct gaggattatt
     9661 gcgaggttgc ggtcgccttc gaagggtagg aagagtctgt ttccgaggtt tcctgtcgtt
     9721 cgtgtgctgg ttgggacgat gcagaggtac tggtcgtttg gctccatcag gatgttgccg
     9781 ctgccgaggt ggatcttgta ggtgcgcacg tcgccctgga cgatcaggaa cctgccttcc
     9841 aggcggcagc ggtccgctat cttgaggcga ggtataagac gttgcagcac ttgtttgcgc
     9901 gtctttgcgg tctgggagag ggcgccgaag gagtagctcg tccagtaatc cctgtagcgg
     9961 ccctctggtc cgccgtcggc ccagttcggg tcgttgccga cgctggctac gcccacgaac
    10021 aggtctacgt ccctcatcac ctcggagagc acgagtggag ggacctcttc gagagcgagt
    10081 ggtcctgtgg gagcgccccg tccacgaaat ccggtggcgt accctccacc tccagcgtgg
    10141 gcgtagttct ccctggcgtc gatgctgtag aagcgcacct ggtccgtggc caggtagagg
    10201 taggtccccg cctccgtggt gtcggcgccg tagttgtcgc cgagaccctc gacccagaat
    10261 tcggccctca atccccaggc cggtagctcc ctggtcgccg gcgggtattc gtcgtcgacc
    10321 atcagcctca gtttgttctt ccacccccgc gccgcgcaaa gggcgttgaa ctggtgctgc
    10381 ttgatcacgt gcgcggcgaa gcgattggag taggttctcg tcgtacgctc tgcgtcggtg
    10441 agcaggtaga cttccctgtg cgcctgcttg aagggctggc gtatctccag ctcggcgagc
    10501 cagtcacgcc aggccaggac ctcgttagga tcctcctgca tggaatgcca cagctctacg
    10561 ctcgtcgagg cgtcgatcca gtccaggacg tggccgttac ggcctacgat ccggccctca
    10621 tagaagacgc cggcttgtga acgtgcttcg tttgtgaact tctagataag gcgccgcgcg
    10681 agcgtgccta cgacaggatg gtcgagatag cgttcgcgcc aggactggta tgtccagctc
    10741 ttctgctcca gacacaggct ctctatcctg tcgcgctggg ccggcagcat cttccgcagg
    10801 tcggtggcga cggctttgat ctccttcagg ttctcggcgc tgtgctcttt gacggctttg
    10861 ggcacggatt tctgcctctt gccgtcgggt ttggtccaac gcagttcggt cgtgctcgta
    10921 ccggttacta ccagctcggc gccgaactcg ccaaacctcg cccgcctcac ccccacgtct
    10981 tgcaggccgt aagtggggac ggagatctcc tctatctcct gcagcggcat tccggcctgt
    11041 cctgccgcgt tgtccagcgc cttctcgatg cctttctgcg ccgtgccgaa cttcaccctg
    11101 gaccgtagca tcgcgagctg ccccacgccc cttgcgtcgg gcatggcgcc caacgcccaa
    11161 acacaggcgt tgccaagcct gacacacctc gggcccatct gtggtgcctt gcgataggcg
    11221 gagagcgcca gtgctcccag cgcacgcgcg acatccgcgt tattgctccc cgcgcaaagc
    11281 caggcgaggg ccttgaggat gtccgcgttc tcgtcctgca agagcaaatt ggggtccggc
    11341 tgccactgtg atccacgctc gacgggcctg gtgcgcggtt tctctaccag ggggaaccag
    11401 cgcacgacgg ccctctcgaa ggcggggcgt ccggtcgtct tcagggctgc gtcagctcgc
    11461 ttcgaccact tctgggtggg cttcgcaccc ctggccccgg tgcagtgttg caggagctcg
    11521 acccaggccg cacgctcttc tccttccatg gcccagatat cgcctatcgc agcatcagac
    11581 cacgcctccc cgggtacgag cggcaggctc gaggccgaaa gcccaccaag gtctttcagt
    11641 ctcgctgccc agcggcgcgt ctcttccgtg ctgtagtctt tttggagact actggtgagt
    11701 tcgttgaccc ttttgcgcag atccggagta aggtcgtgat ccctgaggtg atcctggagc
    11761 accttgatca tctgcggtgt accccgcaca aacgtgtacg gctggcggct ggaccacttc
    11821 aggaggcggc agacgtcgtc atggtcgaag gggagcctgc gcctgagcac ggtctgcatg
    11881 gtctggcgga tcgcccaggt ctcgtggaag gccgcagact gtttgccgtg acggaggtcc
    11941 aaccactcca ccgcggccag gacgaacccg cgctgctctt cgggggttgc cgcaagaatt
    12001 cgacgttccg cctcagattc cctattctgc agggtgtacc gccggtatgt ctcctcctgc
    12061 tcctcggcca ggaggtcgtc gatccaggac ctgtaaggtt gcgggatgcg ccgcctgcgg
    12121 aggaacacgt tcatcctccg acgagcggga cgagcttgag gaaagttacc gtggtgtctg
    12181 gcctgacctc gatctcctct tcgagctcgt ctacctgcct gtcatcgacc agcaatatga
    12241 acccgttggt gtagaatgct ctcaccgcca gctcgaactg cttctcgggg tctatactgc
    12301 gcctcttacg catcttgaag ccgttgaggg acctctctgc gtcggacggc tggatgagac
    12361 cgtggaagta ttcgggctgg tcgaggttgt agtcgtgcac ctccctatag acgcgggcgc
    12421 ggatcaactg ccgcaccgtg atccactcgt ccggtacgtc gagaatgaag tctctctcgt
    12481 catcaccgcc gaggctgaga gtcgtttcgt ccctgatctt cagcgccgtt ggcatagtca
    12541 cactccgtgc tcgaagccct ctggaaacgt acacgaatcg tacggcctgt agcacactac
    12601 gacaagaggc aaaagcgctg caacacacag gattcgaggg tcacctggcg cccgggctca
    12661 cacgagtcaa tcccggtcgt gttctgcttg ctcgcctttc gatgaagctt tggggcgcga
    12721 cctcagaggg acatggtggg atgcatcgtc ttcctcgcca tccgggccgg cgcgccgttc
    12781 agcgcgggtg cgccgtcatc cacatgccgt tgctcggacg cagtgtggcc atggctactg
    12841 attccactcg cctacccggc acgaggtgca ggtcgtagtg ttgtgcaagg agggccagca
    12901 cgagcgtcgc ttccatcagc gcaaagcctt tgccgatgca ctggcgtggt ccacctccga
    12961 aagggaagta cgcgtagtgt gggcgtcggg ccgaacgttc gggggcgaag cgctcggggt
    13021 cgaagccttc ggggttttcc caaagtgttg gatctcggtg tgtgacgtag gggcttatgg
    13081 agacgatcgc gtcggcgggg atgcggtatc cgcaaatggt gtcgtcctgg atcgcccgcc
    13141 ggttggtgat ccaggccggt ggatacaagc gaagcgtctc gtcgaccacc atccgcgtgt
    13201 agggtaggtt gggcaggtcg gcggccgtgg ggatgcgccc ctggagcact tcatcgacct
    13261 cggcacgaag cttgtgcgcg accgccacgt gcgtagagag gagataggag gcccaggtga
    13321 gggcgttcgc cgtcgtttcg tggccggcta cgaagagcgt gatcacctcg tcgcgcagtt
    13381 gcctgtcgct catcccctcg ccggtctcct cgtcgcgcgc ctccatgagg agcgccagca
    13441 agtcgtcgtg ttcgcccggc ctgcgccgtc gttcggcgac gatgccgtag atgacgccgt
    13501 cgagggttgc gcgggccgcc aggaaacgac gattgcgggg cgtcggcacc ttgaggggtg
    13561 gatagaaagg gaacgtgaac cggaaggtaa cctcattcag gagcgtggtg atcgcgtggc
    13621 cgatggcatc tacgtcttgc tctacagagg tgctgaacaa tgcccttgtg gcgacctcca
    13681 acgtcaggtg catgaactcc gtagacacgt cgagtggctg gcccctggcc acgctcggtc
    13741 cccagcgctt tagcatcgcc agggttgcat cggtcatcaa gtcgccgaag gcggcgaccc
    13801 gacgcctgtg gaaggcgggc tgcgccaggc gacgctggcg ccgccagaag tcgccgtcgc
    13861 tggtgaaaag tccgttgccg agaatcaacc tgagcgtccc gaaagttgcg acgtccttcg
    13921 agtagttgcg gtgattgtcg tgcaacaggc gcccgacgcc cgccggatcg ttgacctgat
    13981 acatcctcac gtgcgcgacg cgatactgcg ccacgtcgcc gtacgtgcgt gccacgtcga
    14041 gcacgaagcg cagacggtcg cggtagagat cgccgatgct gcctagcacg acgtttccgc
    14101 gtggtcccgg tggcacttct gcagtggcga tggcctctct gctcgtcgtg cgcatccctg
    14161 gccctcccca acagtttacg ttgggcggat cgtgccgccc gctcctctgt tctatctgtc
    14221 aagacattac tctaccggac gattgtcttc tcaacgacac caccacggaa tctgatggct
    14281 gacatgattc ttggttctgc cagcatggta atggccagag gtaagatcgc cagattccgg
    14341 cggccggcga gcaccgatgg gcataccggg gatgcaccgg ggttctagag gtgagatcat
    14401 cggcacgtcg gtattaaggg gacttcgggc gatgatgagg cgagaggggg gctaaagtcg
    14461 cttggaaaca caggttctat tctttgtcgc cgctgtggtt gcgggcctga tcaactcgct
    14521 cgcgggcggt ggagggctga ttacgttccc gttgctggcc ctggtcgtcc cgccggtggt
    14581 cgccgacgct acgagcgctt tcgccctgct tccggcctac cctagcgcag tatggcggac
    14641 ccgtagcagg ttgcgcgaag tacgacggct gtggctctgg ttgctcctcg ttaccagcgc
    14701 tttgggcgga ctcgtgggag cgctgttgct ggtctggacg ggtgagagga acttgcagtt
    14761 tctcgtgccc tggttggtgt tcggaggcac ggtccttttc gtactggagc cgtggctctc
    14821 tcgccgtagc ggggacgccc acacagaccg cggcctcgcg acggcgctgt ggccgcttgc
    14881 ggcggtcgtg gtgttcgttg tggccgtata cggtggctac ttcggggccg gtatcggcat
    14941 cctgatgatc agcgccctta gcatgctgcg gatgggagac gtacaccgcg ttgttccctt
    15001 caagaacctg ctcgtgggat gcctcagggc cgtagcggtg ttcgtgctcg tgatctacgg
    15061 cgagatcgcc tggagatatg ggtttcctat ggcggtcggc ggtctcatcg gcggttacct
    15121 gggaggcgtg ctaacaggca gggtaaaccg caccattctg cgctcggtcg tcgttacgat
    15181 cgggttcggg ctggctgcgt actacttctg gaccctctac gccccagcag gtctaccctt
    15241 cggcggcgag taggccagac agtcctcgag aaccacgcta tccctt
//
LOCUS       DATMVF010000070         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_102637, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000070 DATMVF010000000
VERSION     DATMVF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102637"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            637..1923
                     /locus_tag="VN178_03095"
     CDS             637..1923
                     /locus_tag="VN178_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS79984.1"
                     /translation="MSERAPLRVVATHDFCCAYPPSPTSYGSLPGGEGLKSTIDALRA
                     QGPIVRADAGDFAAPGALATLSGGEAGFAAAADLGIDVGVVGNHEFEWGVEHLRTHAP
                     QTGFPLLCANAPEVGLPPTAVVETEGGVVGFVGLTCPDPEVYVSAPSLDPDLAGVAIR
                     HAEELRASGAEWVVALLHDGVDWRFGPRGYEAIPERFSEVCRPWAQAVDAIIASHTLG
                     RWIGQIEGTPVVQPWPFGAELGVVELARGEEPRAYCTTSESSGRWSGAGSDLLEEASS
                     RVLGDLVEPLSSRSGGPAPLADFFARALREATGVRAAGVDMLASQPSVDGVLSYLPAG
                     PVTQADVLRLYPWPDATVVGEIGEEELRKVPQASWPWPWSVWGFDATDRSDRGPATLA
                     VLEGDAVAHVERVLGRPVGWRRTEVGLREAVSRALS"
     gene            complement(1956..2834)
                     /locus_tag="VN178_03100"
     CDS             complement(1956..2834)
                     /locus_tag="VN178_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWS79985.1"
                     /translation="MDETTATSEMRVVTLNLWGRRGEWNERKRVLVEGFSELAPDLVA
                     FQEAVVGDGYEQVADILGRSYHIVHQTDREADRGGDIEDGQGISIASRWPLGEVWEPD
                     LNVTPRTEDFACGTLIAEVLAPEPIGPLMFVNHLPNWQLTFEHERELQTVIAARTIEE
                     IAEGREGLHVVLAGDLDATPDAASVRFLRGRQSLGDMSVCYRDAWGSTHPGEEGHTFT
                     AANPLVTAENWDWELELGRRIDYVMVRCSDHGPTLDIRACDRIFDEPADGVWGSDHFG
                     VLADLSAQTSSGRPVH"
BASE COUNT          623 a         1116 c         1172 g          614 t
ORIGIN      
        1 cacggcaacg agctctccgg cctcgtggcg caccgcgaac tggcgatatc cgtcagccac
       61 catctcttcc agcaatcgcg cgtagcgatc ttcgtcgagg gaagggtaca gttcgcggag
      121 gattggtagg gcttctctac gctgtgccac agagcacagt tctactattt ccatagctct
      181 cctccttctg ggcgttgcgt gatctccaat atctcctcag attgtgccca cttggcctag
      241 ctgggtatcg ctagatcttt cggcggtgct atcagtctag gtaagcggat gatctcgcgg
      301 agcaagtacc cgacctcaag gcagctcata atagctcgcg aggagacgca agtggggcga
      361 actactaccg ccttcgtctg ggcccacgca agttaggaca acggcgagca tgaccttcca
      421 ggctttgctc tccggagagc atgttcatct gatggtagcg ctctagccac gcacttgggt
      481 gaacggtcat gttctaacag caaattcgag cgcgatcgct gcacataacg atcacaccct
      541 tggcgtaatg cggctcccca acctttgggg tagccagccc ccgcaagccc cgtacactca
      601 cggcgttgtc catggagata gggggaggat acgctgatga gcgagcgagc accactgcgg
      661 gtggtagcca cccacgactt ctgttgcgcc tacccaccgt cgccgacctc ctacggctcg
      721 ctgccgggcg gcgagggtct gaagagtacg atcgacgcgc taagggcgca agggcccatc
      781 gtcagggcgg acgcgggcga cttcgcggcg cctggagcgc tcgccaccct ttcgggcggc
      841 gaggcgggct ttgcggcggc ggccgaccta gggatagacg tcggcgtggt tggcaaccac
      901 gagttcgagt ggggggtgga gcatctgcgt acgcacgcgc cgcagacggg cttcccgctg
      961 ttgtgcgcca acgcccccga ggtcggactg cccccgacgg cagtcgtcga gactgaaggg
     1021 ggcgtagtgg gcttcgtcgg gctgacctgc cccgatccgg aagtctacgt ctccgcccct
     1081 agcctcgacc ccgacctcgc tggcgtggcc ataaggcacg ccgaggaact gcgggcctcg
     1141 ggcgcggagt gggtggtggc gctcttgcac gacggggtgg attggcgctt cgggccgcga
     1201 ggctacgaag ccatccccga gcgcttctcg gaggtttgta ggccgtgggc gcaggcggtg
     1261 gacgcgatca tcgcgagcca caccttgggg cgttggatcg gccagatcga aggaacaccg
     1321 gtagtccagc cctggccgtt cggggcggag ctcggcgtcg tcgaacttgc ccgaggcgaa
     1381 gaacccagag cctactgcac gacgtctgag tcgagcggcc gatggtctgg ggctggcagc
     1441 gacctactcg aggaagcctc ctcgagggtg ctcggcgatc tcgtcgaacc cctgagctcc
     1501 agatcgggcg gtcctgcgcc gctggccgac ttcttcgcgc gggccttgcg ggaggcgacc
     1561 ggagtccggg ccgctggcgt ggacatgctc gcgtcgcagc cgtccgtgga cggcgtgctc
     1621 tcctacctgc ccgccggtcc cgtaacacag gccgacgtct tgcgcctcta cccctggccc
     1681 gatgccaccg tggtcggcga gatcggagag gaagagctcc ggaaggtacc tcaagcgtcg
     1741 tggccttggc cgtggtcggt gtggggcttc gacgccacgg accgatcgga cagaggaccc
     1801 gcgaccctcg cagtcttgga gggcgacgca gtagcgcacg tggagcgcgt cttgggacgc
     1861 cctgtggggt ggcggaggac ggaggtgggg ctgcgggagg ccgtcagtag ggcactcagt
     1921 tagttcgccc tggcgcatag gggcacgaag ggtggtcagt gcaccggtcg tccgctggag
     1981 gtctgggccg aaaggtcggc gaggacgccg aagtggtcgc tgccccaaac tccgtccgcg
     2041 ggttcgtcga aaatgcgatc gcacgccctg atgtcgaggg tgggaccatg gtcgctgcag
     2101 cgcaccatca catagtcgat gcgcctccct aactccagct cccagtccca gttctcggca
     2161 gtaaccagcg gattagcggc ggtgaaagtg tgcccctcct cccctggatg tgtgctgccc
     2221 catgcatcac ggtagcacac gctcatgtct cccagggact gccgcccgcg caagaagcgc
     2281 acgctcgccg cgtcgggggt ggcgtcgagg tcgccggcca agaccacatg caggccttcg
     2341 cgcccttcgg cgatctcctc tatggtgcgc gccgcgatga ccgtctgaag ctcgcgctcg
     2401 tgctcgaagg tcagctgcca gttgggcagg tggttgacga acatgagcgg cccgatgggc
     2461 tccggggcta gtacctcggc gatgagggta ccgcaggcga agtcctcggt tcggggcgtc
     2521 acgttgaggt ccggctccca cacctcccct agtggccagc ggctcgcgat cgagatgccc
     2581 tgcccgtcct cgatgtctcc gccacggtca gcctccctat cggtctggtg gacgatgtgg
     2641 tagctgcgac cgaggatgtc ggcaacctgc tcgtagccgt cgccgacgac cgcctcctgg
     2701 aaagccacga ggtcgggtgc cagctcgcta aagccctcta cgagcacacg cttacgttcg
     2761 ttccactccc ctcgccgccc ccacaggttg agcgtgacga cgcgcatttc gctcgtagcg
     2821 gttgtctcgt ccatagcgtg cctccctaaa ttcgcttccg cgcaaccata cgaccggaga
     2881 atagtagccc gtacaggcac gaacatccgc cccacctcac gctcagatgc acggcggcgt
     2941 tagcgggtag cgttgtcgcg accgaccgcg tgtcggtcga cgccgattac gacaacggca
     3001 taccgtttag cgtacgccta gacacagagg gtatgatgga cctgcggctc atcgcatagt
     3061 cgcaaggccc gcggggaggc ggtagcagga tgaggcaacc gggccggggt cctcgtttgg
     3121 gcgccccgtc cccgtggtaa ggacctacca agacgcggta ggcgtcgcta gagtatggga
     3181 aggaggccac cacctatgtg tccccacacg cccgaggact actcccccgc ggaagaggtg
     3241 cacaagctgc gcgcaaaaca gaacgaagaa gcccgtggac ggcgcgaaca agcagagcgc
     3301 cggatgacag agaaggaaga agaagcccat gaacgacatg aagatgcgga acgccagatg
     3361 aggggcagga gaaaccccta accgagaccc ccatctctcc ttgattggaa gacgacgcct
     3421 tcgtagcagg ggattctcgc gtcggcagga acatgcatgc agttaggtgt cttcgggtaa
     3481 gcgcggggga ggcgccgggc gggtggcgaa ctactatcac cttct
//
LOCUS       DATMVF010000071         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_377887, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000071 DATMVF010000000
VERSION     DATMVF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377887"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VN178_03105"
     CDS             complement(<1..315)
                     /locus_tag="VN178_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS79986.1"
                     /translation="MSEASIATGPSSVNVDLTKRSAIVTGAASGIGRACAERFSQAGA
                     AVTVLDLDGDAAAEVAEGIGGEAVQADLSDYEVLKSLEIDADIVVNNAGMQHVAAVEE
                     FPP"
     gene            complement(564..1949)
                     /locus_tag="VN178_03110"
     CDS             complement(564..1949)
                     /locus_tag="VN178_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS79987.1"
                     /translation="MPVEPSPRSDAPLVVGGAGACGMVAALAAARCGVQVLVLEKGPE
                     PAGNTARSTGLIPAAGTRFQREAGVLDDSPELMAKDIFEKNNGESDPEVTHLLCRESG
                     PLVEWLVDEFGCEMLCYTDFLYPGQSRHRMHGPKMSYGSELLRQLENAVVEEPLIELR
                     MDTTVGGLLSEGGRVTGVETAEGPIEAGAVVLALNGFGGNREMVERYLGPAAAAALYY
                     GSPNNTGEGIEWGVALGAATGHMGSYQGHASVAAPDGPLVTWGVVVNGAILVNREGRR
                     FGCETVGYSEYAGAVMAQSGGEAWEIFDHEIYEASLRTRFDEVVEAAKVARSETLEEL
                     AETLDLPAGALAETVEDVNSSARGEAPDPFGREDFLGGPLEPPFYGIHVLGALFHTQG
                     GLKVDTHARVLKSDGKHVPGLYAGGGTAVGISGRGYEGYSSGNGLLAAAVLGKVAGEA
                     ASGEILEDLPA"
     gene            complement(1956..3410)
                     /locus_tag="VN178_03115"
     CDS             complement(1956..3410)
                     /locus_tag="VN178_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HWS79988.1"
                     /translation="MSRFDAVVLGGRAVLPGQGLVRADVGIKDGRVSAIGEDLASGDA
                     EETIDAAGKLVLPGAVDAHFHLGIYRDITEDTRSETLSSLAGGVTSIISYFRTGSHYL
                     GKSGPYAEIFPEVLAATNGHSRVDYGYHLAPMVRDQISEIPRLAVEDGVISFKYYMFY
                     KGLDLAGASSDASGYTMSENYDLGHLFEIMEQVAKTQETSDSRVSLSIHCEQPELIRV
                     FSERVRQEGVLSGLEAYSASRPTLTEHLAVAEVGVLAGHTRCPINLLHLSSEEALQAA
                     LELKALDPYLDVRLETTLHHLALTYQNYNDQRGKVNPPIRADSDVDALWRGVVRGEID
                     WICSDHACCSEEHKEGDLWQALPGFGGTALIYPFILTEGPKHGLSLERCAELVATNPA
                     RAYGLSPRKGAIAVGADADLAIVDMEETHPVTTERLLSAQEYTPFEGMELTGWPVHTL
                     LRGRTAFAEGEAVGEPAGTYLERPLTTGKPLAAP"
     gene            3545..>3723
                     /locus_tag="VN178_03120"
     CDS             3545..>3723
                     /locus_tag="VN178_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWS79989.1"
                     /translation="MGGRRALEGIKVIDAATLFAGPLAATVLADFGAEVIKVEHPKGD
                     PSRSHGYSKDGVGLW"
BASE COUNT          655 a         1210 c         1161 g          697 t
ORIGIN      
        1 gggaggaaac tcctccacgg ccgcgacgtg ctgcattccc gcgttgttga ccacgatgtc
       61 agcatcgatc tcgaggcttt tcagcacctc gtagtcagag aggtcggcct gcaccgcctc
      121 tccgccgatt ccttcggcga cctctgcggc agcgtcgccg tccaaatcga gcacggtaac
      181 tgctgcgcct gcctgcgaga acctctcggc gcaggcacgc ccgatcccgc tcgccgctcc
      241 cgtcactatg gcactgcgct tcgtaaggtc cacgtttaca gatgatggtc ccgttgctat
      301 ggaagcttcg ctcatgttcc tccttcatcc aggaagtcaa ccgacctcga caggatcttt
      361 ccccaaaaca caactgagaa accagtttac agcgggtagg ggaagggttg gagctttccc
      421 caccaccgtg ctagtcagaa tctcacgaat cagctttctt gctgggaacc gacaggttga
      481 tggctcgcct agcgcaaagc tcatgcacag cgctgcagag cacagtgttt tgagagatcc
      541 agcgaagggc gtgaagagca cgactatgct ggtagatctt ccagtatctc tcccgaggca
      601 gcctcgccgg cgacctttcc cagcactgcc gcggccaata gtccattgcc gctcgagtag
      661 ccttcgtacc cacgcccgct gatcccgacg gccgtcccac caccagcata tagcccgggt
      721 acgtgcttgc catcggactt gagtaccctg gcatgcgtgt ccactttcaa gccgccctgg
      781 gtgtggaaga gtgcgccgag cacgtggata ccgtagaacg gaggctccag agggccaccc
      841 agaaaatcct cgcgcccgaa ggggtcaggg gcttcgccgc gggccgagga gttcacgtct
      901 tccacggtct ccgccagggc gcctgcggga agatccaagg tctcggccag ctcttccagg
      961 gtctcggacc tcgccacctt ggcggcctct acgacctcat cgaagcgggt acggagggaa
     1021 gcttcgtaga tttcatgatc aaagatctcc caggcctcac cccccgactg ggccatgacc
     1081 gcgcccgcgt actcggagta gccgacggtc tcacacccga accggcgacc ttccctgttg
     1141 acgaggatgg cgccgttcac gacgactccc caggtcacga gcggaccatc cggggcggcg
     1201 accgaggcat gcccctggta ggagcccatg tgtcctgtcg cagcaccaag ggcgacaccc
     1261 cactctatgc cctcacccgt gttgttcggc gaaccatagt acagtgcggc cgcggccgcg
     1321 ggaccgagat atctctctac catctcgcgg ttgccaccga agccgttcag tgccagcacg
     1381 acggctcctg cctcgatcgg accttccgcg gtctcgacac cggtgacccg gccgccttcc
     1441 gagaggagtc cgcccacggt ggtgtccatt cgtagctcta tgagaggctc ttcgacgacg
     1501 gcattctcga gttgccggag gagttcggag ccgtagctca tcttcgggcc gtgcatcctg
     1561 tggcgactct ggcctggata gaggaagtct gtgtagcaaa gcatctcgca tccgaactcg
     1621 tccacgagcc actcgacgag cgggcctgac tccctgcaca gcaggtgtgt gacctccggg
     1681 tcgctctcgc cattattctt ctcgaagatg tctttggcca taagttccgg ggagtcgtcg
     1741 aggacgcccg cctcgcgctg gaatcgggtt ccagcggcgg gtatcagacc ggtagagcgg
     1801 gcggtgttgc ccgctggttc gggccccttc tccagaacga ggacctgtac tccacacctg
     1861 gcagcggcga gagccgcaac catcccgcat gcgcccgcgc cgcccacgac cagtggagcg
     1921 tcgctcctgg ggcttggttc cactggcacc gtaccttatg gcgcggcgag gggcttcccg
     1981 gtggtgagtg ggcgctcaag gtaggtgcca gcgggttccc ccacggcttc cccttcggcg
     2041 aaggccgtcc gcccgcgcag gagcgtgtga accggccagc cggtaagctc catcccttcg
     2101 aagggcgtgt attcctgggc tgagaggagg cgttcggtcg tcacggggtg ggtttcttcc
     2161 atgtccacga tggccaggtc ggcgtcggcg cctacggcga tcgcaccctt gcgcgggctc
     2221 aggccgtagg cgcgcgccgg gttggtcgcg acgagttctg cgcaacgctc tagagagagg
     2281 ccgtgcttcg gtccttcggt caggatgaag gggtagatca aagccgtccc gccgaaaccg
     2341 gggagcgcct gccagaggtc accctcttta tgctcctccg agcagcaggc gtggtcggag
     2401 cagatccaat cgatctcacc ccttaccaca ccgcgccaga gcgcatccac gtctgagtcg
     2461 gcccttatgg gcgggtttac cttgccgcgc tggtcgttgt agttctggta ggtgagcgcg
     2521 aggtggtgga gcgtcgtctc caggcgaaca tcgaggtaag ggtccagagc cttcaactct
     2581 agcgccgcct gtaaagcctc ctcgctggag aggtggagga ggtttatggg gcagcgcgtg
     2641 tggccggcga ggacgcctac ctctgcgacg gcgaggtgct cggtcaacgt cgggcgcgag
     2701 gcgctgtagg cttcgaggcc ggagaggacg ccttcttgcc tgaccctctc cgagaagacc
     2761 cggataagct cgggctgctc gcagtgtatg gagagcgaga cccgcgagtc gcttgtctcc
     2821 tgcgttttcg ccacctgctc catgatctcg aagaggtgac ccaggtcgta gttctcgctc
     2881 atcgtgtagc cgctggcgtc gctcgaagcg ccggcgaggt cgagtccctt gtagaacatg
     2941 tagtacttga aagagatgac gccatcttcg acggcgaggc gagggatctc ggagatttga
     3001 tcccggacca tcggcgccag gtgatagccg tagtccacgc gcgagtgccc gttcgtggcc
     3061 gccagtacct cggggaagat ctccgcgtaa gggccgctct ttccgagata gtggctgccc
     3121 gtcctgaagt agctgatgat gctcgtgacg ccccccgcga gcgatgagag cgtctcgctg
     3181 cgcgtatcct cggttatgtc gcggtagatg ccaagatgga agtgggcgtc caccgcacca
     3241 gggagcacga gtttccccgc ggcgtcgata gtttcctcag cgtcgcccga agccaggtcc
     3301 tcccctattg ccgatacacg cccgtccttg atacccacgt ccgccctgac caggccctgt
     3361 cccggcagaa cggcccgccc gcccagaacg accgcatcga atcggctcat cgccacccct
     3421 tccccgaaga acgcgtagag agtcgccaac atagcacggt gcgcgcggcc cggtcaaaac
     3481 cttgcggtca aacggttcat gagcaaagat aagcgcgacc agtctcggga caggagggcg
     3541 tggcttgggc ggaagacggg cgctggaggg gatcaaggtt atagacgcgg cgacgctctt
     3601 cgccggaccg ctcgccgcga cggtgctcgc cgacttcggg gccgaagtca taaaggtcga
     3661 acatcccaaa ggagacccct cgcgctcgca cggctactcg aaggacggcg tggggctctg
     3721 gtg
//
LOCUS       DATMVF010000072         9716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_378606, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000072 DATMVF010000000
VERSION     DATMVF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378606"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VN178_03125"
     CDS             complement(<1..556)
                     /locus_tag="VN178_03125"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWS79990.1"
                     /translation="MIPQDPVASLVEALGSGAVEVVDLTQPLNERTPVIQLPEQFAQT
                     PAVKRHEISRYDERGPAWAWYWLEIGEHTGTHFDAPIHWISGRDGKDVSQVPPRHLIA
                     PAVVIDRSAESAENPDYLLTVDDVRAFEDEHGPLPEGGWLLLHTGWDARAQDAEAFLN
                     AQDGNPHSPGFDVECARWIAEESPL"
     gene            complement(570..2216)
                     /locus_tag="VN178_03130"
     CDS             complement(570..2216)
                     /locus_tag="VN178_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWS79991.1"
                     /translation="MRVSQAIGRVLVDGGIEAFFGLAGSGNFAVLNALNAAGAAFYSS
                     RHECGAVMMADGYARASRKVGVASVHQGPGFTNTLTGLAEAAKARTPLIVLAADIPAG
                     TLWSNFKVDQASLASTVGAIPERVRRPETAAADTARALRRAQIERRPVVLSIPIDVVE
                     EQCPDGPPAVPHWPPLEPPGPAEGSVAAVADLLALSHRPVIVAGQGAALANAREALEA
                     LGDRVGAVLATSAMGHGLFTGNPYSVGIAGGFSSSLAARLLGRADVVLAFGASLNHWT
                     VRHGNLFPSDARVVQVDLDEEAIGRLYRADVGVVGDAAGAARAIVDELERRGVGGEGF
                     RSDGIEGEISAGRPRDEPYEDLGTAEHVDPRTLTIALDDLLPGERTVCTDSGHFLGYP
                     AMYLDVPDRQGFVFPNAFQSVGLGLASGIGAAVARPERLSVAAVGDGGALMALGEMET
                     AARYRLPMIVVIYNDAAYGAEVHHFGPMGRPVDVARFPDTDFAALARAAGAEGITVRN
                     KGDLSPVRDWLERRDRPLVIDAKVNPEVRAEWLAEAFRTH"
     gene            2536..3783
                     /locus_tag="VN178_03135"
     CDS             2536..3783
                     /locus_tag="VN178_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWS79992.1"
                     /translation="MIIEFQRLLEDVLNRIDREELVRLARELVSIPSVYRPEEPDGNE
                     ARVAGFVADYLESAGFEVKTEEVAPGRPNVWAVWEGDRPGKTLLFEAHTDVVTEGSAE
                     DWDYPPFGAEKDGGRIYGRGACDTKGNLAAAVAAVRALKGSGVPFPGSLILCHPVDEE
                     GMMAGIKAFIERGHAEGVDAAVICEPEENQLCIKQKGALRVEVTLRGTMAHGAMPQSG
                     VNPVTRAARFVVAVEELESEEIERHGEDPFLGYPSLTPTILMGPDSAEPQINVIPASA
                     YVALDIRTVPDQSHRDLVERLEDIIARLASEDPDFDATLEVIEERPPTETPKDEPLVR
                     AMARAYGSLTGEEPAYNGVPGATDGTFLHAWAGIPIVTTGAGDREIPHHADEWVDEEE
                     LLTTCKLYAATAMYYAHGEETNV"
     gene            3776..4558
                     /locus_tag="VN178_03140"
     CDS             3776..4558
                     /locus_tag="VN178_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS79993.1"
                     /translation="MFDYRSMFDLSGKTALVVGAGSGIGEASAQGLAAFGARVVCADV
                     DKEAAEDTATGITSEGGEAEAIELDMTNPDSVRMAVESVGVPDVLVSTPSINVRKPVL
                     DITDDEFDRIVELNLKGTFRLIRDFGRGMAERGSGSIIAFSSIRGQVVEPGQGIYAAT
                     KSGTVQMLRALASEVGPRGVRVNAIAPGIVETPLTAQIKDSPDWYDSYAAKSILGRWA
                     MPHEMVGMVLYLASDASSYVTGAFMLVDGGWTAADGRFTPPL"
     gene            4651..5253
                     /locus_tag="VN178_03145"
     CDS             4651..5253
                     /locus_tag="VN178_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HWS79994.1"
                     /translation="MAMGSGSEGRGSIFRFDTRQIVVAGIIGGIALFLGFTRLGFIPV
                     PIPLIGNATIMHIPAIVGGALEGPVVGLLAGAIFGIFSFLQADNPVFLNPIVAIVPRL
                     FIGVVAWAVFVGLRRWSVDLAAAAAGLLGSFANTVGVLGLAILFGLLPVAVIPAILPQ
                     AIAEAVLAAVVTVVIVRGVMLFRSGRTTAPENESDEERRY"
     gene            5275..6117
                     /locus_tag="VN178_03150"
     CDS             5275..6117
                     /locus_tag="VN178_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS79995.1"
                     /translation="MIELDNVSFTYRGGETDAVPALANIGLKIESGQFVAIIGQNGSG
                     KSTLVKLLTAILYPTEGSIHIDGVPVDKEHRWDVRRKVSVVFQDPDDQLIMNRVIDDV
                     AFGPENMGLSRDEIEERVRSSVRMLGLEEIGGRLIEDLSPGQKQRVAIAGALAMRPLF
                     LILDEPTSLLPVPVAMRLISTIRDLSISENMGVLHVTHSMYEAVLFERVVVMDAGRVI
                     LDGPPAEVFRDVEKLREIGLDVPLAASLAHRLRARGIPIGEDVLNSSDLRAALIALDP
                     SPSG"
     gene            6117..6992
                     /locus_tag="VN178_03155"
     CDS             6117..6992
                     /locus_tag="VN178_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS79996.1"
                     /translation="MRPDGTPPILELKGFDYTYLEGTPRASEAVRDISFRIDSGERVG
                     IVGATQSGKSTVVDSFAHLLRLTPGKVFYKGEDVAAPGYDKGRLRREIGIVFQQPDSQ
                     IIEDVVGADVAFGPTAAGLSAGETRGRVEESLNTLGLPYADFRLRYIHALSGGQRRRV
                     AIAGVLAMHTPVLVLDEPMAGLDPRGRKELLELLIKLQKDRELTLIVCSASLNDASLL
                     CDRFIVLDRGTVVMDGPVREILRQADRLAELDITLPEPVVGAREMQRLFPDFPTDLLT
                     EDELESELLTRLGTA"
     gene            6992..7804
                     /locus_tag="VN178_03160"
     CDS             6992..7804
                     /locus_tag="VN178_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HWS79997.1"
                     /translation="MLELQRNVIFGQFVETGSFIHRMDARVKIAVTSVFIVASFLIDD
                     FLGFAVLLPLLVVIGVVSRIPIGYVLRGSRIFIAFLALILAFQVLFYSRDVPESSYLW
                     RWGLLSVSTEGLYSAGILALRVILLYYVTTMLMLTTSLVDLTNGLELIFGPLQRLRIP
                     VNELVLVFVIAIKFVPIFIEEMERLARAQTARGVPFDEGGAITRARRLGRLLIPIFIS
                     GFARADTLTIAMNTRSYRGGRYRTKLRQMSASPSDWLALILVIAWISVAWLV"
     gene            complement(7972..9078)
                     /locus_tag="VN178_03165"
     CDS             complement(7972..9078)
                     /locus_tag="VN178_03165"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWS79998.1"
                     /translation="MRVFEKLAEYRYEQLVFCHDKATGLRAIIAIHDTTLGPALGGCR
                     MYPYASEDEAIVDVLRLARGMTYKAAASGLNLGGGKSVIIGDPHTDKSEALFRSFGRY
                     IETLGGRYIVAEDVGTSTEDANYIRVETSHVVGVDVTRGGSGDPSPLTALGVLQGMRA
                     CVEEVYGTTSLEGRVVAVQGLGHVGYYLCRHLHEAGANLIVTDVHEAALERVVKEFGA
                     KAVEPDEILTIPCDIFAPCALGAVLDDATIPKLRCRIVAGSANNVLGESGHGEGLSER
                     GILYAPDYVINAGGLINVADELEGYNERRATARVMRIEDRIRRIIAISKRDGVPTNVA
                     ADTLALERIAAISSMERLHTGHPYGQLSRRRETI"
     gene            complement(9186..>9716)
                     /locus_tag="VN178_03170"
     CDS             complement(9186..>9716)
                     /locus_tag="VN178_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HWS79999.1"
                     /translation="VLSVPSEEFERVLDELRGLGEKVTTDSISGQDVTEEYVDLESRE
                     RNLRATEESLLRLYDRAENVEEALSIQRELTTVRGEIELVQGRIKYLDQRSAYSQITL
                     NIQPVTSPPPPKPTWDPGEVVEQAWDASLAVLQVMATAIISAVVFGWWLAPVLIGGLL
                     WWRRRNRDSRPAATES"
BASE COUNT         1701 a         3121 c         3136 g         1758 t
ORIGIN      
        1 cgagagggct ctcctcggcg atccaacgcg cgcactcgac gtcgaaaccg ggcgagtgcg
       61 ggttgccatc ctgcgcgttc aggaacgctt cggcgtcctg ggcgcgcgcg tcccaccccg
      121 tgtgtagcaa cagccagccg ccttcgggta gcggtccgtg ctcatcttcg aacgcgcgca
      181 cgtcatcgac ggtgagcagg tagtcggggt tctcggcgct ctcggcagac cggtcgatca
      241 cgacggccgg ggctatgagg tgccggggag gcacctgcga gacgtctttg ccgtcgcggc
      301 cgctgatcca gtgtatgggc gcgtcgaagt gcgtgccggt gtgctcgccg atctcgagcc
      361 agtaccaggc ccatgcgggc ccccgctcat cgtagcggct gatctcgtgc cgcttcaccg
      421 cgggagtctg cgcgaactgc tcgggcagct ggataaccgg ggtgcgctcg ttcaacggct
      481 gggtcaggtc aacgacctcg acggcaccgc tgccgagcgc ctcgacgagt gacgcgaccg
      541 ggtcttgcgg tatcatctgg tgcctcctct caatgggtcc ggaacgcctc tgcgagccac
      601 tcggcccgta cctccgggtt gaccttggcg tcgattacga gtgggcgatc tcgcctctcc
      661 aaccagtccc gaaccggcga caggtctcct ttgtttctga ccgtgatgcc ctcggccccg
      721 gctgcccgcg cgagcgcggc gaagtcggtg tctggaaagc gcgccacgtc tacagggcgt
      781 cccatggggc cgaagtggtg cacctccgcg ccgtaggccg catcgttgta gatgacgact
      841 atcatcggca ggcgatagcg ggcggccgtc tccatctccc ccagggccat gagagcgcct
      901 ccgtcgccaa cggcggcgac ggagagcctc tccgggcgcg cgaccgcggc cccgatgccg
      961 cttgcgagcc ccaaccccac cgactggaag gcgttgggga acacgaagcc ttgccggtcg
     1021 ggcacgtcga ggtacatcgc cggatagccg agaaaatgcc ccgagtcggt gcaaaccgtg
     1081 cgttctcctg gtagcaggtc gtccagcgcg atggtcaacg tgcgcgggtc tacgtgttcg
     1141 gcggtgccga ggtcctcata aggctcgtcg cgggggcgac cagcggagat ctcaccctct
     1201 ataccgtcgc tcctgaatcc ctcgccgccc acgcctcgtc gttcgagctc gtcgacgatg
     1261 gcccgggcag cccctgccgc gtccccgacg acgccaacgt cggcgcgata gagccggccg
     1321 atcgcctcct catcaagatc gacctggacc acgcgcgcat cggaagggaa cagattgccg
     1381 tggcgcacgg tccagtggtt cagcgacgcg ccgaacgcga gcacgacgtc cgccctcccc
     1441 agcaaccttg cggcgagcga ggaggagaac ccgccggcta tcccgacgga ataagggttg
     1501 ccggtaaaga gcccgtgccc catcgcgctc gtcgcgagca cggcccccac cctatctccc
     1561 agggcctcga gcgcctcccg cgcgtttgca agggcggcgc cctgcccagc gacgatcaca
     1621 ggccggtgcg agagagcgag caggtccgcg actgcggcga cggaaccttc cgccggaccc
     1681 ggcggctcca gcggtggcca gtgcgggacg gccggtgggc cgtcggggca ttgctcttcg
     1741 accacatcga tcggtatgct caggacgacg ggacggcgct cgatctgcgc cctcctgagc
     1801 gccctggcgg tgtcggcggc agcggtctcg ggccgacgca cgcgctcggg gatcgcgccg
     1861 accgtcgatg ccagggaggc ttgatctacc ttgaagttcg accagagcgt ccctgccgga
     1921 atgtctgcgg ccagcacgat cagcggcgtg cgggcctttg cggcctccgc cagacccgtg
     1981 agcgtattcg taaagccagg gccttgatgg acgcttgcga cgccaacctt cctagaggcg
     2041 cgggcgtagc cgtccgccat catgaccgcg ccgcactcgt ggcgggagga gtagaatgcg
     2101 gcgcccgccg cgtttagggc gttcaggacg gcgaagttgc cgcttccggc aagcccgaag
     2161 aacgcttcga taccaccgtc gaccagcaca cgtccgatcg cttgggagac tcgcatgcct
     2221 caggtctccc cacagacaga tagccggccg gtaaagacgg agctagtcac ccgtgcccct
     2281 cctcgttctt cgagcgtgat ccaaagagta tcgcccgaac cataagcgaa cacaggggcc
     2341 cgcgcaacat aggggagatc gcccggcggt tgctcctgga gggcagaacg tggttaaact
     2401 tgaactgtga agcgaggaag gccgcccttt cctgagccgg gcacacttcg gattttgtcg
     2461 gagtgacagc ttgaaaactg tgatgatagg ctgctcttgt ggatttgagt cgactgtcca
     2521 ggccccgaaa cggacgtgat catcgagttc caacgtttgc tcgaagacgt actgaatagg
     2581 atcgaccgtg aggagctcgt gcgcctggcg cgggaactag tcagcatccc tagcgtctac
     2641 cgtcccgaag agcctgacgg aaacgaggcg cgcgtcgccg ggttcgtcgc ggactatctg
     2701 gagagcgcgg gtttcgaggt gaaaaccgag gaggtcgcgc ctggccgtcc gaatgtgtgg
     2761 gccgtatggg agggagatcg tccggggaag accctgctct tcgaggcaca cacggatgtg
     2821 gttacggaag gaagcgcgga ggactgggac tacccgccgt tcggcgccga gaaagatgga
     2881 ggccgcatct atgggcgcgg ggcatgcgac accaaaggga acctggcggc ggccgtggcg
     2941 gccgtgcgtg ctttaaaggg ctccggtgtc cctttccccg gcagcctgat actctgtcat
     3001 cccgtggacg aagaggggat gatggccggg atcaaggcgt ttatcgagcg ggggcacgct
     3061 gagggcgtag acgctgccgt tatctgcgag cccgaggaga accagctctg cataaagcag
     3121 aaaggcgcgc tcagggtgga ggtcaccctg cgcggaacga tggcccacgg ggcaatgcct
     3181 cagagcggcg tcaaccctgt tacgcgggcc gcgaggttcg tagtcgcggt cgaggaactg
     3241 gagagtgagg agatcgagcg ccacggcgag gatccgttcc tcggctatcc gagcctgacg
     3301 ccgaccatat tgatgggccc agacagcgcg gaaccgcaga tcaacgtcat ccccgcgagc
     3361 gcctacgtcg ccttggatat ccgcacggtg ccggatcagt cccacagaga cctcgtagag
     3421 cgactggaag acatcatcgc gaggctagcc tctgaagacc ccgacttcga cgcgacgctg
     3481 gaggtgatcg aagagcgccc gccgaccgag accccgaaag acgagccgct ggtcagggcg
     3541 atggcaaggg catacggtag tctgaccggc gaggagccgg catacaatgg ggtgcccggc
     3601 gcgaccgacg gcaccttcct gcatgcgtgg gccggtatcc ccatcgtcac gacgggagcc
     3661 ggggaccggg agatcccaca ccacgcagac gagtgggtcg atgaggagga actcctcacc
     3721 acctgcaaac tctacgccgc aaccgcgatg tactacgctc acggagagga aaccaatgtt
     3781 tgattaccgc tctatgttcg acctttcggg taagaccgcc ctggtcgtcg gcgccggcag
     3841 cggtatcggc gaggccagcg cacaaggact cgcggcgttc ggcgcgcgcg tagtgtgcgc
     3901 cgacgtggac aaagaagccg cggaagacac tgccacaggg ataacttcgg agggaggaga
     3961 ggcggaggcc atcgagcttg atatgaccaa ccctgacagc gtacgtatgg ccgtcgagtc
     4021 cgtgggtgtt cccgacgtac tggtgagtac gccgtccatc aacgtcagga agcctgtgct
     4081 cgacataacg gacgatgagt tcgaccggat cgtcgagctg aacttgaagg ggactttccg
     4141 cctgatccgg gactttggac gcggcatggc cgagcgcggc tcgggcagca tcatcgcctt
     4201 ctcgagtatc aggggacagg tcgtcgaacc gggacagggg atctacgcgg cgacgaagtc
     4261 aggtacggta cagatgctgc gggccctcgc ttccgaagtc gggccccgcg gcgtcagggt
     4321 aaacgccata gccccaggca tagtcgagac gcctttgacg gcccagatca aagactcccc
     4381 cgactggtac gactcctacg cagccaagag catcctgggc cgctgggcca tgccgcacga
     4441 gatggtcggg atggtcctct acctcgcctc ggatgccagc tcctacgtca cgggcgcctt
     4501 catgctggtc gacggcggct ggaccgccgc ggacggacgc tttacgccgc cactctaaag
     4561 cggtttgaca ggatgtgtgc acacacggat actaaatgaa tccggcacat agtcatttcg
     4621 gaagccggta ctgacaagga ggagagggtc atggcgatgg gttcggggag cgaagggcga
     4681 ggctccattt tccgcttcga caccaggcag atagtggttg ccggcataat aggtggcatc
     4741 gcgctgtttc tcggtttcac gcggttgggc ttcatcccgg taccgatacc gctcataggc
     4801 aacgccacga taatgcacat accggcgatc gtcggtgggg cgctggaggg acccgtcgtc
     4861 gggcttctgg caggtgcgat cttcgggata ttctcctttc tgcaggcgga caacccggtc
     4921 ttcctcaacc ctatagtcgc catcgtgccg cgcctgttca tcggggtcgt ggcgtgggcc
     4981 gtcttcgtgg ggctgcggcg ctggagcgtg gacctcgctg cggccgcggc cggattgctg
     5041 ggatccttcg ccaatacggt gggggtgctc ggcctcgcca tactcttcgg attgcttccc
     5101 gttgccgtca ttccggccat cttgccgcag gccatagcag aggccgttct ggccgcggtc
     5161 gtaacggtgg tcatcgtcag gggcgtgatg ctcttccgca gcggcaggac cacggccccg
     5221 gagaacgagt cggacgaaga acgtcgctac tagtcggggc gcggcgcggg cgcgatgatc
     5281 gagctggaca acgtctcctt cacctaccgt ggtggcgaga ccgacgctgt tccagcgctt
     5341 gcgaacatcg gcctgaaaat agaatcgggg cagttcgtcg ctatcatcgg ccagaacggc
     5401 tccgggaaga gcacgctggt caagttgctc accgccatcc tctacccgac tgaaggtagt
     5461 atccatatag acggcgtacc cgtggataag gagcaccggt gggatgtgcg gcgcaaggta
     5521 tccgtcgtct tccaggaccc cgacgaccag ctgataatga accgcgtgat agacgacgtg
     5581 gctttcggcc ccgagaatat gggactctcg cgggatgaga tcgaggagag ggtacgctcg
     5641 tccgtgcgta tgctcggcct cgaggagatc ggtggtcgcc tgatcgagga cctctcgccg
     5701 ggccagaagc agcgggtggc catagcaggg gccctggcga tgcgtcctct gttcctgatt
     5761 ctggacgaac cgacctcgct cttgccggtg ccggtcgcga tgcgcctgat ctctaccata
     5821 agggatttga gcatcagcga gaacatgggt gttctgcacg tcacccactc aatgtacgag
     5881 gccgtcctct tcgagagggt cgttgtcatg gacgcggggc gtgtgatcct tgacggaccc
     5941 cctgccgagg tcttccgaga cgtcgagaag ctgcgggaga tcggcctgga cgtaccactc
     6001 gccgcgagcc tcgcccaccg cctgcgcgcc cgcggcatcc cgataggaga agatgtcctg
     6061 aactcatccg acctgcgcgc cgccctcatc gccttagatc cttcgccctc aggctgatgc
     6121 ggccagacgg aacacctcct atcctggagt tgaagggctt cgactacacc tacctggaag
     6181 gcaccccgag ggcgagcgag gcggtaaggg atatttcctt caggatcgat agcggcgagc
     6241 gcgtcgggat agtcggtgcc acccaatcgg gcaagtccac cgttgtcgac tccttcgcac
     6301 acctgctgcg cctgacgccc ggaaaggtct tctacaaagg cgaggacgtg gccgctcccg
     6361 gctacgacaa gggcaggctg cgccgcgaga tcgggatagt gttccagcaa ccggactcac
     6421 agatcataga agacgtggtc ggcgcggacg tcgccttcgg accgaccgca gcaggcctct
     6481 ccgccggtga gacccgcggg cgcgtcgaag agagcctgaa taccctggga cttccttatg
     6541 cggatttccg cctgcgttac atccacgcgc tctccggcgg ccagcgcagg cgggtcgcca
     6601 tagccggggt gctcgccatg cacacgcccg tcctggtcct cgacgagccg atggcggggc
     6661 tggacccgcg tggtcgaaag gagcttctgg aactcctcat caaattgcag aaggatcggg
     6721 agctgaccct gatcgtctgc tccgcgagcc tcaacgacgc aagcctgctg tgcgaccgct
     6781 ttatcgtttt ggaccggggc acggtcgtca tggacggacc cgtgcgcgag atcctgcgcc
     6841 aggccgacag actcgccgag ctggacataa ccctgcccga gccggtcgtc ggcgcccgcg
     6901 agatgcagag actcttcccc gacttcccga ccgacctgct gacggaggac gagctcgaat
     6961 ccgagctgct aacgaggttg gggacagcgt agtgctcgag ctgcagcgta acgtcatctt
     7021 cggtcagttc gtcgagacgg gctcctttat ccaccgcatg gacgcccgcg tcaagatcgc
     7081 cgtgacatcc gtgttcatcg tcgcctcgtt cctcatagac gacttccttg gcttcgccgt
     7141 cttgctgcca ctcctcgtcg tcattggcgt agtctccagg ataccgatcg ggtacgtact
     7201 tcgaggctcg cggatcttca tcgcgttcct ggcccttatc ctcgccttcc aggtgctctt
     7261 ttactcgaga gacgtgcccg aatcgagcta cctgtggcgg tggggcctac tctcggtctc
     7321 caccgagggc ctctactcgg ccggcatcct cgcacttcgt gtcatcctcc tgtactacgt
     7381 cacgaccatg ctgatgctca cgacctcgct cgtcgacctg acgaacgggt tggagctcat
     7441 cttcggcccg ctacagcggc tgaggatacc ggtcaacgag ctggttctcg tcttcgtgat
     7501 cgccataaag ttcgtcccca tctttatcga ggagatggaa cgtctggccc gcgcccagac
     7561 ggcccgcggc gtccctttcg acgagggcgg cgccattacc agggcgcggc gcctcggacg
     7621 cctgctcatc cccatcttca taagcggctt cgcccgcgcc gataccctaa ccatcgccat
     7681 gaatacccgc agctaccgcg gcggccgcta ccgcaccaag ctccgccaga tgagcgcgag
     7741 tccctccgac tggctggcgc ttatactggt tatcgcatgg ataagtgtgg catggctggt
     7801 gtagagcact tcagcacgcg ccctgtcggg cgcttgtcag cacttcagct agtcaggttt
     7861 cgcgtgcgcg agtcttggct gaaagggtgg ctcagccgcc gggctgacaa gccgccaatg
     7921 gcggcgtgct gaagtgctga caaggcggtg gagccgccgt gctgacatgc tttaaatcgt
     7981 ctcccgcctg cggctcaact gcccgtaggg gtgccccgta tgcagccgct ccatcgagct
     8041 tatggcggct atcctctcca gcgccagggt gtccgcagcg acgttggtag ggacgccgtc
     8101 gcgtttggag atggcgatga tgcggcggat gcggtcttct atgcgcatga cccgcgcggt
     8161 cgcacggcgc tcgttgtagc cctccagctc gtcggccacg ttgataaggc cgccggcgtt
     8221 tatgacgtag tcgggggcgt agagtatgcc gcgttcggag agaccctcgc cgtggcccga
     8281 ctcgccgagc acgttgttgg cgctccccgc gacgatgcga cagcgcaact ttggtatggt
     8341 cgcgtcgtcg aggacggcgc cgagggcgca aggggcgaag atgtcgcagg ggatggtcag
     8401 gatctcatcc ggctcgaccg ccttggcgcc gaactccttg accacgcgtt ccagggccgc
     8461 ctcgtgcacg tcggtcacga tcaggttagc acccgcctcg tgcagatgac ggcacaggta
     8521 gtaaccgacg tgccccagac cctgcacggc gacgacccgg ccttccaggg aggtcgtgcc
     8581 gtagacctcc tcgacgcagg cccgcatacc ctgcaagact cccagggcgg tgagtggcga
     8641 ggggtcgccc gagccgccgc gggtgacgtc gacccccacg acgtgtgagg tctcgaccct
     8701 gatgtagttg gcgtcctccg tcgaggtacc aacatcttcg gccacgatgt agcgtccgcc
     8761 aagggtctcg atgtagcgcc cgaacgagcg gaagagcgcc tcggacttgt ccgtgtgggg
     8821 gtccccgata atgacggatt tcccaccccc aaggtttagg ccgcttgcgg cggccttgta
     8881 tgtcatccct ctcgccaggc gtagcacgtc cacaatggcc tcgtcctcgc tggcgtaggg
     8941 gtacatcctg catccgccta gagccgggcc gagcgtggtg tcgtggatgg cgatgatagc
     9001 ccgtagcccc gtcgccttgt cgtggcagaa cacgagctgt tcgtagcggt actccgccag
     9061 cttctcgaat actcgcaagc cctgtctcct ttcccgggcc agaggttccc caggaatcta
     9121 ttctactacc cctgggcttt ctcaaagcca atccttccgc acaccacggc ttcaggtttc
     9181 agatgtcagg actctgttgc agcgggtctg gagtccctgt tccgccgcct ccaccacaga
     9241 agccccccaa taagcacggg tgcgagccac cagccgaata caaccgccga gattatggcc
     9301 gtcgccatga cctggaggac agcgagcgag gcatcccatg cctgctccac gacctctcca
     9361 gggtcccagg taggcttggg aggcggcggg ctcgtgacgg gttggatgtt cagggtgatc
     9421 tgggagtagg cgctgcgctg gtccaggtac tttatcctgc cctgcacgag ctctatctcc
     9481 ccccgaacgg tcgtcaactc gcgctggatc gagagcgcct cctcgacgtt ctcggccctg
     9541 tcgtagagcc tcagcaggct ctcttcggtg gccctgagat tgcgctcccg ggactcgaga
     9601 tccacgtatt cctcggtcac atcctgcccg ctgatagagt cggtcgtgac cttctcgccc
     9661 aggcctctca actcatcgag caccctctcg aactcctcgg aggggaccga gagtac
//
LOCUS       DATMVF010000073         5818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_285797, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000073 DATMVF010000000
VERSION     DATMVF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285797"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VN178_03175"
     CDS             complement(<1..660)
                     /locus_tag="VN178_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWS80000.1"
                     /translation="MREADAKRAPVIGTLRVLLVEDSENDAMLLLRELRRGGYEPLSQ
                     RVYTPEDMEEALRSADAGGEPFEVVVSDYYMPRFRAPDALRVLRDLGYDLPFIVVSGK
                     IGEDAAVGIMKAGADDYVTKENMSRLCPAIERELREAQVRRDRERAEKELMRSEDRFR
                     HLVEQIPAITYVQEPIDSDTPKAITYMSPQYEAMLGYPPEKEMLDEEHWLRVLHPDDR
                     ER"
     gene            complement(653..2635)
                     /locus_tag="VN178_03180"
     CDS             complement(653..2635)
                     /locus_tag="VN178_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HWS80001.1"
                     /translation="MRIVRWKGSWSGERDVSGGGCVRGRVLHVARGSESLIGRFGAYL
                     LVLRSGAGRLLRHLRLAAAAYGVLLISLLLAGLAWYYVRQTVEAQDRVRFDETVQATQ
                     AAVDRRTDAYLDALFGGRGVFLASEAIEREEWESYVEGIETKSRLEGLQALGFARYVR
                     PEEREAFAGEARREELPEMRPDLDPGGERSAYFPLALVAPTDEANLSMINQDAYTEQA
                     HRVAMDRARDTGSPQATRMIYVLTESNTNSSADLVMSPGFAVYLPVYAEGEPVERVAA
                     RRRVLQGFVVGYFRQDGLLDDVFGSGFDPAIDFELYDGADVASSSLLYDEDGVERASE
                     EGYDPLFSDENRFWVAGREWSLYFATLPGFEKGAESNLPAFVLASGIGASVLLFGISW
                     MLVRSRILAERASKDLEVANRELEGTNKELEAFSYSVSHDLRAPLRAIDGFSQILQED
                     YEHALDDEGMDYLGRVRAASGHMGTLIDDLLDLSRVGRRPLRRERVDLTHLATEIVEE
                     LKESQPDREVEFVAAEDIMAWGDASLLKLALENLLGNAWKFTARESAARIEFGAEKRP
                     GPDSASPVYYVRDNGAGFDQTYADKLFGAFQRLHGQDEFEGTGIGLATVARIVHRHGG
                     RVWAEGTVGEGATFYFTLGGRDIGDRSVPAKRAEVA"
     gene            complement(2705..3250)
                     /locus_tag="VN178_03185"
     CDS             complement(2705..3250)
                     /locus_tag="VN178_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80002.1"
                     /translation="MSQLPSMTYGLFSYLLLACVSAVVGYYFARKRTEYEVRYRHRVE
                     VAEGVQGMVTPLVEEFEAALEYVRAPGPSRELPIEEIERSVDGLEKYLAQQEMWLESR
                     SCAALADLIGSFRARGRMLGLLPLRYDDPDFEKAYEQAGAELDEWLRADLPASRERLA
                     DAFREMLGVKKWRRKVLRSPL"
     gene            complement(3247..3900)
                     /gene="lexA"
                     /locus_tag="VN178_03190"
     CDS             complement(3247..3900)
                     /gene="lexA"
                     /locus_tag="VN178_03190"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWS80003.1"
                     /translation="MGEALHPRRVEILRYLARRASMEGPPPSVREIGAAVGLSSSQTV
                     HHHLGRLEEEGYVSRMGRGARTLGLTGKGWEAAGHMALLGRIAAGRGLEAVATDEAYS
                     LAAELLASRSGKRRYLLRVVGQSMVGARIEDGDLLVVEEDESPPDGSIVVVLLREGEE
                     VTVKRFFREGEVLRLRPEAPPGEYEEMVVPAGEVRVQGRVVYVLHPPGRRPGGGIRA"
     gene            complement(3963..4934)
                     /locus_tag="VN178_03195"
     CDS             complement(3963..4934)
                     /locus_tag="VN178_03195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80004.1"
                     /translation="MMRPPEGYTTRSPAREDAEAVAALISTCQIADTGASDISVEEMI
                     DDWHSLDLDEEAVAVIAQDGTIVAYADVFNRSFVIVSIYGYVHPDYRGVGLGSYLVAW
                     GERWTLDHMPQAQQNARVVVQHYINSANEAGRLLLESSGYTPVRGIYVMETTLDEAPP
                     PPHWPPDISVRTFVPDQDERAVYEAVEDAFRDLWGRPRNPFERFIGEKEVESFDPSLW
                     FLAMDGDEIAGVILCKTLAGEGWVNVVGVRRPWRKRGLGLALLRYALAEYQRRGVEKV
                     ALSVDAESITGAPRLYGRAGMRVRESYIIHLKELRPGVDLGTRLDED"
     gene            complement(5010..>5818)
                     /locus_tag="VN178_03200"
     CDS             complement(5010..>5818)
                     /locus_tag="VN178_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS80005.1"
                     /translation="IFLGPTGVGKTELARTLAEYLFGEEDAMIRIDMSEFQEGHTVSR
                     LVGAPPGYVGYEEAGQLTEQVRRRPYSVVLFDEIEKAHPDVFNTLLQVLDDGRLTDAK
                     GRTVNFENTVVIMTSNVGSQHLVSTRQFGFTPRDGVDFRETERRAREALERAFRPEFL
                     NRVDEIIVFQPLTKEDVLKIVDIMLSRLNKHLESQRVQIEVTDAAKEFLAEEGYDPKF
                     GGRPLARAIRRYVENPLSSRIIGGEFGPGDTVVVDRAEEGLAFKVKVPVS"
BASE COUNT          990 a         2062 c         1694 g         1072 t
ORIGIN      
        1 cctttctcta tcatcgggat gcagcaccct gagccagtgc tcctcgtcga gcatctcctt
       61 ctcgggcgga taaccgagca tagcctcgta ctgggggctc atgtaagtta tggccttcgg
      121 ggtgtcggag tcgatcggtt cctgaacgta ggtgatggcc ggtatctgtt ctacgaggtg
      181 cctgaagcga tcctcgctcc gcatcagctc cttctccgcc cgctcgcgat ccctcctcac
      241 ctgcgcctcc ctgagctcgc gctcgatggc ggggcacagc ctgctcatgt tctccttggt
      301 cacgtagtcg tcggccccgg ccttcatgat ccccaccgcc gcgtcctctc ctatcttgcc
      361 tgagacgacg atgaaaggga gatcgtaacc gaggtcccgc agtaccctca gggcgtcggg
      421 cgccctgaag cgtggcatgt agtaatcgga gaccacgacc tcgaaaggct cgcctccggc
      481 gtccgccgaa cgcagtgcct cctccatgtc ttctggggta taaaccctct gcgagagggg
      541 ctcgtatccg ccacgtctca gctcccgcag gagaagcatc gcgtcattct cggagtcctc
      601 gacgagcagc acgcgcaggg tccctatcac cggcgccctc ttcgcgtcgg cctcacgcaa
      661 cttcggccct cttcgcaggg accgaacggt ccccgatatc ccgaccaccc agcgtgaagt
      721 agaacgtggc cccctcgccc accgttccct cggcccagac tctgccgccg tgcctgtgga
      781 cgattcgggc taccgtcgcc aggcctatcc ccgtcccctc gaactcatcc tgcccgtgca
      841 gcctctggaa cgcgccgaag agcttgtctg cgtacgtctg gtcgaaaccg gccccgttgt
      901 cccgcacgta gtagacgggg gacgctgaat ccggaccggg tctcttctcg gcgccgaact
      961 ctatgcgcgc cgcactttcc cttgcggtga acttccacgc gttgccgagc aggttctcga
     1021 gcgccaactt gagcaggctc gcatctcccc aggccataat gtcctctgcg gccacgaact
     1081 cgacctcccg gtctggctgc gactccttga gttcttcgac tatctccgtg gcgagatggg
     1141 tcagatccac gcgttcgcgg cgcaggggcc tgcgtccaac cctagagagg tcgagcaggt
     1201 cgtctattag ggtgcccatg tgcccgctcg ccgccctcac gcgaccgaga tagtccatcc
     1261 cctcgtcgtc gagcgcgtgc tcgtagtcct cctgcaagat ctgggagaag ccgtcaatgg
     1321 ccctcaaggg ggccctcagg tcgtgggaga cggaatagga gaacgcctcg agctccttgt
     1381 tggtaccttc cagttccctg ttggccacct ccaaatcctt gctcgcgcgc tcggccagga
     1441 tacggctgcg cacgagcatc caggagatgc cgaagagcaa gacgctggct cctatgccgc
     1501 tggccagcac gaaagccggc aggttgctct cggcaccctt ctcgaatccc ggcaacgtgg
     1561 cgaagtacag gctccactct cgcccggcca cccagaagcg gttctcatcg gagaagagcg
     1621 gatcataccc ctcctcgctg gcgcgctcca ccccgtcttc gtcgtagagg agcgagctcg
     1681 acgcgacgtc ggcgccgtcg tacaactcga agtctatggc cgggtcgaag ccgctcccga
     1741 acacgtcgtc gagaagcccg tcctgcctga aatacccgac gacgaacccc tggaggacgc
     1801 gcctccgtgc ggcgaccctc tccacaggct ccccctcggc gtagaccgga aggtaaacgg
     1861 cgaagccggg gctcatgacg aggtcggcgc tcgagttcgt gttcgactcc gtgagcacgt
     1921 agatcatcct ggtagcctgc ggagagcccg tgtcgcgcgc cctgtccatg gcaaccctgt
     1981 gggcctgctc cgtgtaggcg tcctggttta tcatgctcag gttggcctcg tccgtagggg
     2041 cgaccaacgc gagcgggaag taggcggaac gctccccgcc gggatccagg tcgggccgca
     2101 tctcgggcag ctcctccctc ctggcctccc cggcgaaagc ctccctctct tcgggcctta
     2161 cgtacctggc gaagccgagc gcctgcaggc cctccaaccg gctcttggtc tctatgccct
     2221 cgacgtagct ctcccactct tcgcgctcga tcgcctcgct ggccaaaaac accccgcggc
     2281 cgccgaacag cgcgtcgagg taagcatccg tccgccggtc cacggccgcc tgcgtagcct
     2341 ggacggtctc atcgaaacgg accctgtcct gcgcctcgac cgtctggcgc acgtagtacc
     2401 aggccagccc ggcgagaagg agagagatca agagcacccc gtaagccgcc gcggcgaggc
     2461 gcaggtggcg caagagccgg cccgcccccg accgcaaaac gagcaggtaa gccccgaacc
     2521 tgcctatgag agactccgac ccacgagcaa cgtgcagtac acggccacgg acgcatccgc
     2581 ccccgctaac gtcgcgctca ccgctccacg aacctttcca tcggactatc cgcacgggac
     2641 gccccgtata aagccatgtt acccggcaca cagtcggccc gcaagatttg gcgtcctcct
     2701 cgggttagag aggtgaccgc aggactttgc gccgccactt cttcacgcct agcatctccc
     2761 gaaacgcgtc ggccagtcgt tcacgggatg cggggaggtc agcacgcagc cattcgtcta
     2821 gctcggcccc ggcctgttcg taagccttct cgaagtccgg gtcgtcgtag cgcaggggga
     2881 gaagacccag catcctcccc cgggcgcgga agctcccgat aaggtctgcg agggcggcac
     2941 aggatcggct ctccagccac atctcctgct gcgcgaggta cttttccagg ccgtccacgg
     3001 agcgttcgat ctcttcgatc ggcaactccc tcgacgggcc aggagcccgt acgtactcca
     3061 gagcagcctc gaactcttcg acgagcgggg tcaccatccc ctgaacacct tcggcgacct
     3121 caacccggtg ccggtaacgg acctcgtact cggtcctctt ccttgcgaaa tagtacccga
     3181 cgaccgcgct cacgcaggcg agcagcagat acgaaaacaa cccgtaagtc atcgacggaa
     3241 gctggctcac gcccggatgc cccctcccgg cctcctcccc ggaggatgca acacgtagac
     3301 cacgcgtccc tgcacccgca cctcccccgc cggtaccacc atctcctcgt actctcccgg
     3361 aggagcctcg ggccgcaacc tcaacacctc cccctccctg aagaagcgtt ttaccgtcac
     3421 ctcctcgcct tccctcaaca acaccacgac tatggaacca tccggcgggc tctcgtcttc
     3481 ctccacgacc agcaggtccc cgtcctcgat cctcgccccc accatgctct gtcccaccac
     3541 ccgtaggaga tacctgcgct tccccgaacg cgaagcgaga agctcggccg ctaatgaata
     3601 cgcctcgtcg gtcgccaccg cctcgagccc gcgtccggcg gcaatcctgc cgagcaacgc
     3661 catgtggccg gccgcctccc agcccttccc cgtcagcccg agcgtcctcg cacccctccc
     3721 catcctcgac acgtatccct cttcctccaa cctgccaagg tggtggtgta cggtctgcga
     3781 actactcaag ccaacggcag ccccgatctc ccgcacgctc ggcggaggcc cctccatact
     3841 cgcccgccgc gcaaggtagc gtaggatctc cacccgcctc ggatgtaacg cctctcccac
     3901 aggtataccc tagcgcaacg gaaggcttta gtcaaacatt tgtttggtgc actgggatga
     3961 ggctaatctt catcgagtct cgtacccagg tccacgcccg ggcgaagctc tttgaggtgg
     4021 atgatgtagc tctctctgac gcgcatgccg gcgcgtccgt agagtctggg ggcgccggtg
     4081 atgctttcgg cgtccacgct gagggcgacc ttttcgacgc cccggcgttg gtactcggcg
     4141 agggcgtagc ggaggagggc gagtccgagc ccgcgcttcc gccacgggcg cctcaccccg
     4201 acgacgttga cccatccctc gccggccagc gtcttgcaga ggatgacgcc cgctatctcg
     4261 tcgccgtcca tggccagaaa ccagagggaa ggatcgaagc tctccacctc cttctccccg
     4321 atgaaccgtt cgaatggatt gcgagggcgg ccccacaggt ctctgaacgc atcctcgacg
     4381 gcctcgtaga ccgcacgctc gtcctggtct gggacgaagg tgcgcacgga gatgtcaggg
     4441 ggccagtgtg gtggtggcgg ggcctcatcg agggtggtct ccatcacgta tattccgcgc
     4501 accggggtgt agcctgagct ctccagcagc agtcgtccgg cttcgttggc cgagttgatg
     4561 taatgctgca cgacgacgcg tgcgttctgt tgggcttgtg gcatgtggtc gagcgtccag
     4621 cgctctcccc acgcgacaag atagctaccg agccccaccc cccgatagtc cgggtggacg
     4681 tagccgtaga tcgagacgat cacgaaggag cggttgaaca cgtcggcgta ggcgacgatg
     4741 gtgccatcct gcgcgataac ggccacggcc tcctcgtcga ggtctaaaga gtgccagtcg
     4801 tcgatcatct cctccacgct aatgtccgat gcgccggtgt ctgcgatctg gcaggtggag
     4861 ataagggcgg caacggcctc cgcgtcctcg cgcgcgggcg atctcgtcgt gtagccttct
     4921 ggcggccgca tcatggtgag aaacctagca taagagagag actccccgaa atggggagcc
     4981 tctctctttg gattccgttg ataggaacct tatgacactg gcaccttgac cttgaaggcg
     5041 aggccttcct ccgcgcggtc cacgacgacg gtgtcgccgg ggccgaactc tccgccgatg
     5101 atgcggctgg agagggggtt ctcgacgtag cgccttatgg cccgcgccag cggccttccg
     5161 ccgaacttcg ggtcgtagcc ctcctcggcg aggaactcct tggcggcgtc cgtcacctcg
     5221 atctgcacgc gctggctctc gaggtgcttg ttcaggcggg agagcatgat gtccactatc
     5281 ttcagcacgt cctccttggt gaggggctgg aagacgatga tctcatctac cctgttgagg
     5341 aactcgggcc tgaaagcccg ctccagggcc tcgcgcgccc gccgctcggt ctcccggaag
     5401 tccacgccgt ctctcggggt gaagccgaac tgcctcgtcg agacgaggtg ctggctgccc
     5461 acattcgagg tcatgatgac gaccgtgttc tcgaagttga ccgtccggcc cttggcgtcc
     5521 gtcagacgcc cgtcgtcgag cacctgaagc agcgtgttga agacgtcggg gtgggccttc
     5581 tcgatctcgt cgaaaaggac gacgctgtaa ggacgcctgc gcacctgctc cgtgagctgt
     5641 cccgcctcct cgtagccgac gtagccggga ggtgcgccga cgaggcggct caccgtgtgg
     5701 ccctcctgga actcggacat gtctatccgg atcatggcgt cctcctcgcc gaagagatac
     5761 tcggcgagcg tacgcgccag ctcggtcttg ccgactcccg ttgggccgag gaagataa
//
LOCUS       DATMVF010000074         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_104166, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000074 DATMVF010000000
VERSION     DATMVF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104166"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..881
                     /locus_tag="VN178_03205"
     CDS             201..881
                     /locus_tag="VN178_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HWS80006.1"
                     /translation="MSESTERKVPLALMVQAPDEPGVLHALTGVFLEHEANITHVDIA
                     ERREGLTTVYFELEDVAGDAVDVLLGDLQALPVVRAAERAPSFQKVYGKRIIVVGGGA
                     QVGQVVVGAVAEADRHNIRGEKISVDTIPLVGEEELAAATRAVARLPRAVALVLAGAL
                     MGGEVTRAVHEIREKGIIVLSLNMPGSVAEAADLVVSDPVQCGVMAVMAVSDSALFDI
                     ARQRGQKY"
     gene            982..1647
                     /locus_tag="VN178_03210"
     CDS             982..1647
                     /locus_tag="VN178_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS80007.1"
                     /translation="MRLVIFGPTGGTGRRLVERAIAEGHEVTAFVRNPSRMSARHGRL
                     KVVVGDASDAGKVHEAVAGNEAVISVLGSRQPSNPLHPRRAGDPHGPVSAGSANIIAA
                     MEEYGLRRFVCQTAWGVGESKEDPGFAGAFFMKVLVPPLLRDEYAEKEAQEKLLRESD
                     LDWVIVRPMLLTNGPWTNDYRVGLDLKPGRRPYISRADVADFLMRQLTDDTFVHKTPS
                     ISY"
     gene            1932..3674
                     /locus_tag="VN178_03215"
     CDS             1932..3674
                     /locus_tag="VN178_03215"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWS80008.1"
                     /translation="MDTLSDNFVRAARLDDVRAAGRTSVRVAGHSLALFFHDGRVHAL
                     DNRCPHMGFPLHRGTLQDGILTCHWHHARFDLESGGTFDQFADEARVFPVEIRDGEVW
                     VDLSPREDRPAYLRQRLRDGLERNISLVMSKSAIGLLGSGEDPAEPFRVGLEFGSRYR
                     RAGWGQGLTMHTCMMNLLPHLDPEDRPRALYHGLSAVSRDSAGEPPRFSLRPLPTETV
                     DLTTLEAWFRRFVEVRDSEGAERCIVSAIRTGAGPAQMADLLFSAATDHRYLDSGHIL
                     DFTNKALEALDIAGWSHAEVVLSSLAEGYVGARRMEESNSWRNPVDLVQILEAAFERL
                     PNALETGRDRRGSWKGREELAPVLLGEDGGAIAGALLEALQDGATEEELAGAVAYAAA
                     LRIARFPTTNEFGDWDTSLHTFTFSNAVHQGLRRAPSPELLRGVFDAAMSVHLDRFLN
                     VPPSRVPEPNGLEAEPEDLLAELPVLLDERQKVDASGELVAGYLYGGGDADRLLAVMG
                     GLLLREDRNFHTIQAVEAAFRQYDHLRGATAGAHVLVAAARYLAAHSPTMRSQGQTYD
                     IARRLSRGEILYEE"
BASE COUNT          695 a         1150 c         1233 g          635 t
ORIGIN      
        1 ctgacgatta cggtatgcct gctgatcgcg ggaattggcg ccgcccgcga cgcccacgtc
       61 ggtacatcca tcccggaaga agcgcaggga taagtgctcg tccgggaaga ctccactttc
      121 aagagcgtag atgggtctgg accgttcgat gccggttcgc aacgaggaag tggacgcggg
      181 aaagctatac actccggcct atgagcgaga gcaccgagcg taaggtcccc ctggccttga
      241 tggtccaggc gccagatgag cccggcgtcc tccacgccct gaccggggtg ttcctcgaac
      301 atgaggcgaa cataacccac gtcgacatag cggagcggcg tgaagggctc accacggtct
      361 atttcgaact agaggacgtc gccggcgatg ccgtggacgt gctcttagga gacttacagg
      421 ctttgcccgt cgtgagggcg gcagaacggg cgccctcgtt ccagaaggtg tacggcaagc
      481 ggatcatcgt ggtcggcgga ggggcgcagg tgggccaggt ggtggtcggc gcggtggcgg
      541 aggcagacag gcacaacatt cgcggggaga agatctccgt cgacaccatc cccctggtcg
      601 gcgaggaaga gctcgccgcg gcgacacgcg ccgtggcccg actgccgcgg gcggtagcgc
      661 tcgtcctggc cggtgcgctg atgggtgggg aggtcacccg cgcggtacac gagatccgag
      721 aaaaagggat catcgtgctc tcgctcaaca tgccaggtag cgtcgcagag gcggccgacc
      781 tcgtggtgag cgacccggtc cagtgcggcg tgatggccgt tatggcggtc tccgattcgg
      841 ccctcttcga tatcgctcgc caacggggcc aaaaatacta gtcagctcag cgtctaccgg
      901 cagtcgtaga tcgggttccg gagagaaatg ggtacactcg agcacgttag cttgcgacgg
      961 cggatgatgg ggagtgcgac gatgaggttg gtgatcttcg gaccgacggg cggcaccggg
     1021 cggcgtctcg tggagagggc gatcgcggaa ggacacgaag taaccgcttt cgtcagaaac
     1081 ccctccagga tgagcgcacg tcacgggcgg ctaaaggtgg tcgtgggtga cgcctccgac
     1141 gcggggaagg tgcacgaggc ggtggccggc aacgaggcgg tgatcagcgt cctcggctcc
     1201 cggcaaccaa gcaacccgct tcaccctcgc agagcggggg acccgcacgg accggtttcg
     1261 gccggctccg ccaacatcat cgcggcgatg gaggaatacg gtctgcggcg cttcgtgtgc
     1321 cagacggcgt ggggggtggg ggagagtaag gaggatcccg gttttgcggg cgcatttttc
     1381 atgaaggtgc tcgtgccgcc gctgctcagg gatgagtacg cagagaaaga ggcacaggag
     1441 aagctcttgc gggagagcga tctggactgg gtcatcgtac gccccatgtt actgaccaac
     1501 gggccgtgga cgaacgacta ccgcgtgggg ttggatctga aacccggcag gcgtccctac
     1561 atctcgcggg cagacgtcgc cgacttcctt atgaggcaac tcaccgacga cacattcgtt
     1621 cacaagacac cgtccatcag ctattgaccc ctacgcacga ccattcgaac gatcccgcag
     1681 ccgtataccg aatccgattg aagggaaccg cgtccgggct gcgggcaaac atggaggtcc
     1741 ctcccatgcg tcattcccgc gcaagcggga atccacgggt tgaaagaggg cggtggtcga
     1801 tagcttgctc cgtcgtgccg ttggcattca ccaggacttg gtatcacagg aagatctacg
     1861 atagaggaag gatctggact ccgcaccgtt cgtgtcatac agtgacgtgc agaagggaaa
     1921 ggggttacat cgtggacact ctctcggata acttcgtgcg ggccgccagg ttggacgatg
     1981 tgcgggccgc cggtcgcacc tctgtccgtg tcgccggcca ctcgctcgct ctgttcttcc
     2041 acgacggtag ggtgcacgcc ctggataatc gctgtcccca catgggcttt cctttgcacc
     2101 ggggcaccct gcaagacggt atcctcacct gccactggca tcacgcccgc ttcgatcttg
     2161 agagcggcgg aaccttcgac cagtttgccg acgaggcgcg cgtctttccg gtggagatca
     2221 gagacggcga ggtatgggtg gacctctcgc cgcgggagga ccgtccggcc tacctgcgcc
     2281 agcgtctgag agatgggctg gagcgcaaca tctcgctggt catgtccaag tcggccatcg
     2341 ggttgctcgg cagcggtgaa gaccccgccg aacctttccg cgtcgggctg gagttcggca
     2401 gcagataccg gcgggcaggg tggggtcaag gactgaccat gcacacctgc atgatgaacc
     2461 tcctgcctca cctggacccc gaggaccgac cacgggccct ctaccacggg ctctccgccg
     2521 tctctcgaga ctccgctggg gaaccgccac gtttctccct gcgtccgctg cccaccgaga
     2581 cggtggacct gaccacgctc gaggcctggt tccgccggtt cgtcgaagtg cgcgactcag
     2641 aaggtgccga acgctgcata gtctctgcga tccgcacagg agcaggtccg gcccagatgg
     2701 cagatctact cttctctgcc gccaccgacc accgctacct ggatagcggg cacattctgg
     2761 acttcaccaa caaagcttta gaagcactcg acatcgccgg atggtcgcat gccgaggtgg
     2821 tcctctccag cctggccgaa ggatatgtgg gtgcgaggcg catggaggag tcgaactcct
     2881 ggcgcaaccc cgtggatctc gtacagatac tcgaggccgc cttcgagcgg ttgcctaacg
     2941 ctctggagac cggccgggac aggcgcggaa gctggaaggg tcgcgaggaa ctcgcccccg
     3001 tgctgttggg agaggatggg ggagccatcg ccggggcgct actcgaagcg ctgcaggacg
     3061 gcgccaccga agaggagctg gcaggggcgg tggcctacgc cgcagcgctg cgcatagcgc
     3121 gctttccaac cacgaacgag tttggcgact gggatacctc cctgcacacc ttcaccttct
     3181 ccaacgccgt ccaccagggg ctgcggcggg caccctctcc agagctgctc cgaggggtct
     3241 tcgacgccgc gatgagcgtc cacctcgacc gcttcctcaa cgtcccgccc tcacgtgtgc
     3301 cggagcccaa cgggctggaa gcggagccgg aagatttgct ggcagaactt ccggtgttgc
     3361 tggacgaacg gcagaaggta gacgcatcgg gggaactggt cgccggctac ctctatggcg
     3421 ggggagacgc tgaccggctc ctggccgtga tgggcgggct gctcctaagg gaggaccgca
     3481 acttccacac catacaggcc gtcgaagccg ccttcaggca gtatgaccat ctgcgcggcg
     3541 ccacggcggg cgctcatgtg ctggttgcgg ccgcccgtta cctggcagcc cactctccca
     3601 ccatgcgttc ccaaggacag acctacgata tcgcccgccg cctctctcgc ggcgagatcc
     3661 tgtacgaaga gtaagcccga ccaatccgtc acggtgtaac cacgtgcgga caa
//
LOCUS       DATMVF010000075        12781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_604648, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000075 DATMVF010000000
VERSION     DATMVF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12781
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604648"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..791)
                     /locus_tag="VN178_03220"
     CDS             complement(45..791)
                     /locus_tag="VN178_03220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS80009.1"
                     /translation="MGSLQDRVAVVTGASSGIGAAVARTLNAEGAHVALAARRKGALL
                     EIQAGLDSGDGKESMVVPTDVTDRGQVRSLVAGVEEELGPVEILVNCAGVMYYTLMKN
                     RREAEWELTVEVNCKGALNCIGAVLSGMLARGRGHIVMISSDAGRKVFPGLAVYSASK
                     FFVEALSQGLRLETAGTGVKVTTIQPGNVATDLIEMSGDEEALEEHGYPGESRVLDPE
                     DVAASVVHALVQPEHVAVNEILVEPRDEPV"
     gene            complement(948..1523)
                     /gene="cysC"
                     /locus_tag="VN178_03225"
     CDS             complement(948..1523)
                     /gene="cysC"
                     /locus_tag="VN178_03225"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWS80010.1"
                     /translation="MNEHRGFTLWFTGLSGAGKTTISEIVEHELRERERQVEVLDGDI
                     VRTNLSKGLTFSRDDRNINVLRIGFVANLLTRNGVGVIVSAISPYKEARDQVRRRIVD
                     FVEVFVDAPLEVCAERDVKGLYKKAFAGEIEQFTGVSDPYEPPNAPDLVLKTDEETPE
                     ESARRVIEKLEFFGYLWPREAEATEYNWSSR"
     gene            complement(1587..2888)
                     /locus_tag="VN178_03230"
     CDS             complement(1587..2888)
                     /locus_tag="VN178_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWS80011.1"
                     /translation="MPDLPNILYIHSHDTGRWVRPYGYSVRTPNIQRLAEEGVLFRKA
                     FCAAPTCSGSRACLLTGQYAHSNGMVGLAHRGFSLKDYRQHIVHTLRDLGYRSALIGE
                     QHISKKPDIIGYDEVVKISTSHTDDVVPVTLDLLERASDRPFFLSVGFFETHREWFRP
                     SSPKKANYVIPPPNLPDAPETRLDMAAFQESARSLDRGIGAVLDALDAEGLAEDTLVI
                     CTTDHGLAFPGAKATLYDRGLGVMLILRGPGGFTGGKASDALVSHIDLFPTVCDLTGV
                     EKPEWLQGESLLPLVSGEAEEVRDAIFAEKTYHVAYEPERCVRTHRWKYIRRFGERTR
                     PVLPNTDDGPSKELLLTYGWGEREIPPERLYDLVLDPNEVNNLAGDPAHAGTLGELRE
                     RLDAWMVETKDPLLEGDVPAPEGAELNDPDGLSPNDPTIIV"
     gene            complement(2894..3784)
                     /locus_tag="VN178_03235"
     CDS             complement(2894..3784)
                     /locus_tag="VN178_03235"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HWS80012.1"
                     /translation="MGDGSWLAGPALSYLICATPRSGSTLLCVALDDTGIAGHPEEHF
                     EVLLETGQPRQPRDYFQRSNDPEVWALLDDPEFRDVLGEYGGRYAEHPARRDPRWRPP
                     DFERLVEEALRKGTTENGVLGTKIMWAYFRDFVRLARRTTSPDVPPCEVPASVLPNLR
                     RFVWMRRSDTTRQAVSLWKALQTQQWRKDSDEDVSGQGLRFSFAAVDHLKLRIDEHNA
                     AWQDFFEVCGVEPMEVVYEELVEDYRGTVLWLLDGIGIPVPENFAVAEPRMRRQADEL
                     SEEWVRLYDERAAAKTVQKG"
     gene            4025..4189
                     /locus_tag="VN178_03240"
     CDS             4025..4189
                     /locus_tag="VN178_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80013.1"
                     /translation="MKWDLDLWYELWDKAGSQGLDTWYDLRGEVRDLPDTGHTPDAGP
                     DVIEDGREGI"
     gene            4308..4460
                     /locus_tag="VN178_03245"
     CDS             4308..4460
                     /locus_tag="VN178_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80014.1"
                     /translation="MVGAEAVFFSTIVAAWLVLPAVVGLKNVLDAGCFYMLISLGIVV
                     VISVAS"
     gene            complement(4492..6435)
                     /gene="sat"
                     /locus_tag="VN178_03250"
     CDS             complement(4492..6435)
                     /gene="sat"
                     /locus_tag="VN178_03250"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HWS80015.1"
                     /translation="MTQNKPVALWAVPRSISTAFERVFVERDDFEVLHEPFSASYYYG
                     EDRPSDRFADAAPKPEYSYERVVEDVFRPRGQRVFLKDMAYHAKRLLSPGFASQFVNT
                     FIVRDPKYVLVSLYKMWPDFTLEETGYEDLYRLFRYATDAGEDVAVVDAMTFSENPAG
                     VLAAYCEHLEVPFTAGSLTWESGEVSEWDNWEGWHEDAESSTGIEPAERLDPELPGEL
                     EAAYEHCLPYYYELAAHAIPSVARRTVVGRTEDDKEKKLLRTELAIAPHGGDLVDRVV
                     REEERRERSMEAAGLQKVPLSARALSDLQMISTGVFSPLEGFMLREEYEGVVEDMRLQ
                     DGLSWSLPVTLSADEEKARELSENSEIALVDGAGEPVATMVLRELYRYDKEREAKMVY
                     RTTDADHPGVAAVYRQGEVLLGGEVELLRPPDEGRFPRYYYTPAQLRASFAEKGWKRI
                     VGFQTRNPVHRAHEYIQKSALETVDGLLLNPLVGETKSDDIPADVRMRSYEVILDGYY
                     PEDRTMLAVFPAAMRYAGPREAIFHAICRKNYGCSHFIVGRDHAGVGSYYGTYDAQHI
                     FEEFEPEELGITPLFFEHAFFCTECEGMGSSKTCPHDPDSHVFLSGTKVREMLSKGEY
                     PPPEFSRPEVIEVLIEGQRTHSR"
     gene            complement(6797..8248)
                     /locus_tag="VN178_03255"
     CDS             complement(6797..8248)
                     /locus_tag="VN178_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWS80016.1"
                     /translation="MRKKPKRTFTRRDFLRAAGAGATLLGAAGCAGLTDRVPRLPEEY
                     LPGGGTGSNVILVIIDTLRKDHVGAYGNDWIQTPTLDALAEESMLFNRAYPEAMPTIP
                     VRRAIHTGMRTWPTRPPSFGWKPIPSGHTTVAEILSKEGFGTYLVTDTYVQFPMNFRR
                     GFGTYNVIRGQERDPYKDPLTISEEEMRDRYIILGEGKKARQYLANVQDRKGEEDWFA
                     PRVFLDAMDALEAAAGENRPFFLVADCFDPHEPWDPPQEYVSLYDEGYEGREPLDDNY
                     GTDDYLTDRQLLRMRALYAAEVTMMDRWLGKFIERAHELGVMEKTLFVVVSDHGHLLG
                     EHGYTGKLANALYPELTDIVLMMRHPQGEGAGKSSDFYASTHDVAPTILGFLGVEQAD
                     PMDGQDLRTILGGKEPVQPRDHFTQGYKNYVCCRDEHRVMFCRTDMTDAHLFDAVNDA
                     QQRRDLAEAEPETVKRMYEEYAQQDAAGRLPNF"
     gene            complement(8363..9055)
                     /locus_tag="VN178_03260"
     CDS             complement(8363..9055)
                     /locus_tag="VN178_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80017.1"
                     /translation="MKESDQDYLFEQFRVDRGCLGYIVADARSAAIFDPELEMVEPML
                     DFIFEHGLKPAYIVDTHTHADHISGARELKLKTTAKIVMHEKAPASCVDMRVEDGDRL
                     LVGDVSIKFIHTPGHAKDLVSVMLPGRILTADALLIGSCGRTDLPNGNAVRQYHTLYY
                     TYRALPDDLLVFPGHDYNGREHSILKEEKESNPKMLFDTEEEFVEYMERKNPGELTPV
                     YHLADALKANMA"
     gene            complement(9093..10238)
                     /locus_tag="VN178_03265"
     CDS             complement(9093..10238)
                     /locus_tag="VN178_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HWS80018.1"
                     /translation="MHSFRTTVSVPRNASGAASVTERGIRKSLPPERFVDLREGEAKP
                     GDPTGDLAYNAEMRWEAMAEEGCLTPVESFFVRNHAATPSIDPGFWTLHVEGPGVERP
                     LRLTYDDLSSMQQVSIVQALECAGNGRAFFEELQARRPEGTPWGLGAVGVAEWTGVML
                     REILERAGLKASAHGVMLESLDAVRMRRPLPVEKAVEEGTLLAFSMNGAMLTPDHGFP
                     LRAIVSGWAAVASVKWLGRIYVSEKQLFSPWNTDKYVLTGGESGAAREPVEERGVKSA
                     LELAWPARLQEGRHVLGGRSWSGYGAISRVEYSVGGGPWREARLFGENTPGAWARWSF
                     EWDAKPGEHEIRVRATDNAGNVQPDEVGWNDLGYLYDGVVGHPIEVV"
     gene            complement(10274..10417)
                     /locus_tag="VN178_03270"
     CDS             complement(10274..10417)
                     /locus_tag="VN178_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80019.1"
                     /translation="MLTANLVFLVGLATISFFLPLSDFQRWFVLSAAAVLAAVDWAAV
                     ERL"
     gene            complement(10757..11077)
                     /locus_tag="VN178_03275"
     CDS             complement(10757..11077)
                     /locus_tag="VN178_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80020.1"
                     /translation="MGKLQHGRFWAFWRKCERAPAYDASGNERGSPPDMKGPTMLVAD
                     RAGHSSGLWDFAEFALRHAACEEELLEVRLDEVSIMCWCLRCKELRIFAKARYVDDPA
                     GCPE"
     gene            complement(11618..>12781)
                     /locus_tag="VN178_03280"
     CDS             complement(11618..>12781)
                     /locus_tag="VN178_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWS80021.1"
                     /translation="YTTMGVVDTPFYIRNGFGYDRGFDDFVWGRVQGDDTRPHERADA
                     RSTWRGEEDRMVARTVAQAERWLERHHDEQFFLYVDTWDPHEPWDAPDYYTRKYREGY
                     DGEQIYPAYGNWKKAGLKKDDVDLGHATYCGEVTMVDFWIGRLLAKLDALGLRENTLV
                     LFTSDHGFYFGEHDYFGKAEWVHDPEAEVSKDSSLPEWLQDSWLLTVERSPLYKELTN
                     IPLIVRGPGLEPGRTQAMTTAPDIAPTIMDLVGLGGVPTTMTGDSFGGVLDGTRDEHR
                     PLVVSSWPLYLAKGEIITAVDSKARRIDDYMPLTVTTPERSLILGGPDDEPELYDLAR
                     DPQERENAWATSGEGALLAEQALSFLEGVRTPEEYLAPRYEAVEKWRAVRKTA"
BASE COUNT         2250 a         4194 c         3767 g         2570 t
ORIGIN      
        1 gtggggaaga tcttcaaccc ggcctccttt cctatgaacg ttcctcagac cggctcgtcg
       61 cggggctcga ccaggatctc gtttacagcg acatgctctg gttgcacgag cgcatgaacc
      121 accgacgcgg ccacgtcctc aggatcgagc accctgctct cgccgggata tccgtgttcc
      181 tccagcgcct cctcatctcc gctcatctcg atgaggtccg tcgccacgtt gcccggttgg
      241 atggtcgtca ccttcacccc cgtgccggca gtctccagtc tcaagccctg ggagagcgcc
      301 tccacgaaga acttgctcgc cgagtagacc gcaagaccgg gaaagacttt tcttccggcg
      361 tccgatgaga tcatgacgat gtgcccccgc cctctcgcca gcatcccgga caacaccgcg
      421 cctatgcagt tgagcgcgcc tttgcagttg acctcgacgg tcagctccca ctccgcctcg
      481 cgccggttct tcatcagggt gtagtacatg actccggcgc agttgacgag gatctccacc
      541 ggaccaagct cctcctcgac gccagccact agcgaccgca cctgcccgcg gtctgtcaca
      601 tctgtcggca ctaccatgct ctccttgcca tcaccactat cgagaccagc ctggatctcc
      661 agtagcgccc cttttctgcg ggcagcaagc gccacgtgcg ccccctccgc attcagagtc
      721 cgggcgacag cggcgccgat gccgctcgac gcccccgtca ccacagcaac cctgtcttgc
      781 agagacccca cctctacgcg gaaactaccc tctcacaacg ctattcacaa gaatcgttcc
      841 tgctttccca atgagctgga atgctgacaa gcgaaggcga agcctgagcg tgccgaaatg
      901 ctgaccagcg tggcaccggg aaggctcggg ccttcccggt gccgctttca gcgagagctc
      961 cagttgtact ccgtcgcctc cgcctcccgg ggccagaggt acccgaagaa ctccagcttc
     1021 tctatgaccc tgcgggcgct ctcttcggga gtctcctcat cggtctttag cacgaggtcg
     1081 ggagcgttgg gcggctcgta ggggtccgag acgccggtga actgctctat ctccccggca
     1141 aaagccttct tgtataagcc cttgacgtcc ctctctgcgc acacctccag cggagcatcg
     1201 acgaagacct cgacgaagtc tacgatcctg cgccgcacct ggtccctcgc ctctttgtac
     1261 ggcgagatgg cgctgacgat cacacccacc ccgttgcgcg tgagcaggtt cgcaacaaag
     1321 ccgatcctga gcacgtttat gttgcgatca tccctggaga aagtgaggcc cttggacagg
     1381 ttggtcctca cgatgtcccc gtccagcacc tccacttgcc gctccctctc gcgcagttcg
     1441 tgctcgacta tctcggatat ggtcgtcttg cccgctccgg atagaccggt gaaccacaac
     1501 gtgaatccgc gatgttcgtt catatacgtt cctttcgacg ttccggctgt ctatagccgt
     1561 agtttacatt tccctcaggg ccttgctcac acgatgatcg tgggatcgtt gggggagagc
     1621 ccgtcggggt cgttgagctc ggcgccctct ggcgcaggca cgtccccctc gaggagaggg
     1681 tctttcgtct cgaccatcca ggcatcgagt ctttcacgca actccccaag cgtccccgcg
     1741 tgggcgggat ctccggcgag gttgtttacc tcgttgggat cgaggaccag gtcgtagagt
     1801 cgctcggggg ggatctcgcg ctctccccat ccataagtga gcagcaactc cttgctcgga
     1861 ccgtcgtcgg tgttcggcag gacgggcctg gtgcgctctc cgaagcgccg gatgtacttc
     1921 cagcggtggg tgcggacgca ccgctcgggc tcgtaggcga cgtggtaggt cttctcggcg
     1981 aagatggcgt ccctgacctc ctcggcctcg ccgcttacga gcggtaggag cgactcgccc
     2041 tgcagccact ccggcttctc gacaccggtg agatcacaga cggtcggaaa gagatctatg
     2101 tgcgagacga gggcgtcgct cgcctttccg cctgtgaaac cgcctggtcc gcgcaggatg
     2161 agcatcacgc cgagacccct gtcataaaga gtggccttcg cgcctgggaa ggcaaggccg
     2221 tggtcggtcg tgcagatgac gagcgtgtcc tcggccaggc cttcggcgtc gagggcgtcg
     2281 agaacggccc ctatgcccct gtcgagcgag cgggcgctct cctggaaggc ggccatgtcc
     2341 aggcgcgtct cgggcgcgtc aggcagattg ggcgggggaa tgacgtagtt ggccttcttc
     2401 ggtgaactcg gacggaacca ctcgcggtgg gtctcgaaga acccgacaga gaggaagaag
     2461 ggcctgtcac tcgcgcgttc cagcaggtcg agcgtcacgg gcacgacgtc gtcggtgtgg
     2521 ctggtgctga tcttgacgac ctcgtcgtaa ccgatgatgt cgggcttctt ggagatgtgc
     2581 tgctcgccta ttagcgccga ccgataccca agatctcgca gcgtgtgaac gatatgctgc
     2641 ctgtaatcct tgagcgagaa gccgcggtgg gcgaggccga ccatcccgtt ggagtgggcg
     2701 tactgcccgg tgagcagaca tgcccggctt cccgaacacg taggggcggc acagaacgcc
     2761 ttgcggaaga gcacgccctc ctcggcgagc cgctggatgt tgggcgttct cacggagtag
     2821 ccgtaaggcc tgacccaccg cccggtgtcg tgggagtgta tgtagaggat gttcgggaga
     2881 tccggcaact actttatcct ttctgcacgg ttttcgcggc agcgcgctcg tcgtacagac
     2941 gaacccactc ctcggagagc tcgtcagcct gccgcctcat tctaggctct gcgacggcga
     3001 aattctcggg gacggggatc cctatgccat cgagaagcca taacaccgtc ccccggtaat
     3061 cctcgacgag ctcctcgtag accacctcca tgggctccac gccacacacc tcgaagaagt
     3121 cctgccaggc ggcgttgtgc tcgtcgatac gcagcttcaa atggtctacg gcggcaaagc
     3181 tgaaacgcag gccctgtccg ctaacgtcct cgtcggagtc cttgcgccac tgctgggtct
     3241 gcagcgcctt ccacaacgag acggcctggc gggttgtgtc actacgacgc atccagacga
     3301 accgtcgcag gttgggcaaa acggaggcgg gcacctcgca gggaggtaca tcgggcgaag
     3361 tcgtccgccg cgcaaggcgg acgaagtccc gaaagtaggc ccacatgatc ttggtcccta
     3421 acacgccgtt ctcggtcgtg cccttcctca gcgcctcttc caccagcctc tcgaagtcgg
     3481 gcgggcgcca gcggggatcc cttcgcgccg gatgctccgc gtacctgcct ccgtactcgc
     3541 cgagaacatc cctgaactcc ggatcgtcaa gcagcgccca cacttcggga tcgttggagc
     3601 gctggaagta gtcgcgtggt tgcctgggtt gccccgtctc gagcaagacc tcgaagtgct
     3661 cctccgggtg gcccgcaata cccgtgtcgt cgagcgccac gcaaagcaac gtgctcccac
     3721 tgcgtggcgt cgcacagatc aaatacgaca gcgccggccc agcgagccag gatccatcac
     3781 ccataaacct tcctcccacg tacctcgata atgtctacta aaactatctt gtctattaca
     3841 atagtaaaga atacacgaaa ccctttatgg atcaacactc ctagatgccg aatccacccg
     3901 tacctcccga cccctagtaa gtgggcgaaa acaaaccccg gcgcttgaca ccgtcagggc
     3961 ggttgtatac cataatgtct acttataaag tagacaatgg gcgaatggag tggaggtagg
     4021 ggagatgaaa tgggatctgg atctctggta cgagctttgg gacaaggctg gcagtcaggg
     4081 gttggacacc tggtacgacc tcaggggtga ggtacgtgat ctcccggaca ccggacacac
     4141 gccggatgcc ggacctgacg ttatcgaaga tgggcgagag gggatttgac ggtcctttgt
     4201 tcaaagcagc tgcaaagggc cgagcgcatg ttgacggtgg cgcggtccgt ggttggcttg
     4261 agtcggtcgt tttggcttcg ctgctcttct gcgaggtggc gtctgcagtg gtgggagctg
     4321 aagcggtatt cttctcgacc atcgtagccg cctggctggt cttaccggcc gttgtgggac
     4381 tcaagaacgt cctcgacgcc ggatgcttct atatgctcat ctcgctcggc atagtagtgg
     4441 taatcagcgt cgcgagttga acaagtcagc acgcgcttcg cgtgctgact gctacctgct
     4501 gtgggtcctt tgcccttcta tgagcacttc gatgacttcg ggacgggaga actcgggagg
     4561 cgggtactcg cccttggaga gcatctcgcg gaccttggta ccagagagga agacgtgcga
     4621 gtcggggtcg tgcgggcagg tcttggatga tcccataccc tcgcactccg tgcagaagaa
     4681 cgcgtgctcg aagaagagcg gtgtgatccc cagttcctcc ggctcgaact cctcgaagat
     4741 gtgctgggcg tcgtaggtcc cgtagtagct gccaacgcca gcgtggtccc ggcccacgat
     4801 aaagtgcgag caaccgtagt tcttgcggca gatggcgtgg aagatcgcct ccctgggacc
     4861 ggcgtagcgc atcgccgccg ggaagacggc gagcatcgtg cggtcttcgg gatagtaccc
     4921 gtccaggatc acctcgtaag acctcatcct gacgtcggcc ggtatgtcgt cggatttcgt
     4981 ttccccgacg agcgggttca agaggagccc gtccaccgtc tcgagggcgc tcttctggat
     5041 gtattcgtgc gcccggtgga cggggttcct ggtctggaag cccacgatcc gcttccaccc
     5101 cttctcggcg aacgaggccc tgagctgcgc cggggtgtag tagtagcggg ggaaacgtcc
     5161 ctcatcgggc gggcgcaaga gctctacctc accgcctagc aagacctcgc cctgcctgta
     5221 gaccgccgcg acccccggat ggtctgcgtc ggtcgtcctg tagaccatct ttgcctcgcg
     5281 ctccttgtcg taacgataga gctccctgag caccatggtc gccacgggct ccccagcacc
     5341 gtcgaccagg gctatctcag agttctccga cagctccctg gccttctcct cgtccgcaga
     5401 tagggtaacg ggcaggctcc aggacagacc gtcttgcagg cgcatgtctt cgaccacgcc
     5461 ctcgtactct tcgcgcagca tgaatccttc cagggggctg aagaccccgg tcgagatcat
     5521 ctgcagatcc gaaagggccc gcgcactcaa gggcaccttc tgtagcccag cagcctccat
     5581 cgaccgctcc ctgcgttcct cctcgcggac cacgcggtct acgaggtccc ccccatgcgg
     5641 ggctatcgcg agttctgtcc ttagcaattt cttctccttg tcgtcttccg tacgtcccac
     5701 gacggtccgc cgcgccacgg atgggatcgc gtgcgccgcc agctcgtagt agtagggtaa
     5761 acagtgctcg taggcggcct cgagctcccc gggcaactcg ggatctaggc gctcggccgg
     5821 ctctataccg gtgctgctct cggcatcctc gtgccatccc tcccagttgt cccactcgct
     5881 cacttcaccc gactcccaag tgagggagcc tgccgtgaaa ggcacctcga ggtgctcgca
     5941 gtaggccgca agcacgcccg ccggattctc cgagaaggtc atcgcgtcga ccacggcgac
     6001 gtcttcaccc gcgtccgtcg cataacggaa cagcctgtaa aggtcctcgt agccggtctc
     6061 ctccagcgta aagtcgggcc acatcttgta cagcgacacc aatacgtact tggggtccct
     6121 gacgataaac gtgttgacga actgcgaggc gaacccggga ctcaacaacc tcttcgcgtg
     6181 gtaggccatg tccttgagga agacgcgctg ccctctcggc ctgaagacgt cctcgaccac
     6241 cctctcataa ctgtactcgg gcttcggcgc agcatccgcg aagcggtcac tcggacggtc
     6301 ctccccataa tagtaggagg ccgaaaacgg ctcgtgcagg acctcgaagt cgtccctctc
     6361 cacaaagacc ctctcgaacg ccgtagatat cgagcggggt acagcccaca acgcaacggg
     6421 tttgttctgt gtcacacgcg cctctctctc gtactcaaca gggtgagcca gactccgccg
     6481 gttctaccac gcccgatgcc gggatacgcc acaacgtcac aggagcatac cgccaagcct
     6541 attcattttg cctactagat ttgtgagcat cgaaagactg tagcccagca tcgtttcgag
     6601 gtcaagcgta atagcacttc agcacgctca ggcttttagc cttcacttgt cagcacgctt
     6661 cgcccttcgg gcttcgcttt cagcatgtca gccaggactc gaggacgcga aagctgaaat
     6721 gctgaagtgc tgacaagcga cagccggagg ctggagcgtg ctgacgagcg gaggcgttag
     6781 ccggagcgcg ctaatctcag aaattcggca gccgccccgc ggcgtcttgc tgcgcgtact
     6841 cttcgtacat cctcttgacc gtttcgggct cggcttcggc caggtccctt ctctgctgag
     6901 cgtcgtttac ggcgtcgaag aggtgggcgt cggtcatgtc ggtgcggcag aacatcaccc
     6961 tgtgctcgtc cctgcagcag acatagttct tgtagccttg ggtgaaatgg tccctcggtt
     7021 ggacgggctc cttacccccc aggatggtcc tcagatcctg gccgtccatg gggtcggcct
     7081 gctccacccc gaggaaacca aggatcgtcg gggcgacgtc gtgggtggaa gcatagaagt
     7141 cgctgctctt gccggccccc tcaccctgtg ggtgcctcat catgaggacg atgtctgtca
     7201 gctctgggta gagggcgttt gcgagcttcc ccgtgtagcc gtgctcaccg aggaggtggc
     7261 cgtggtcgga gacgaccacg aacagggtct tctccattac cccgagctcg tgcgccctct
     7321 cgataaactt cccgagccaa cggtccatca tggtgacctc ggcggcgtag agtgccctca
     7381 tcctgaggag ttggcggtcg gtcaggtaat catctgtgcc gtagttgtcg tccaggggct
     7441 ccctaccctc gtagccttcg tcgtagaggc ttacgtactc ctgtggcggg tcccagggct
     7501 cgtgcggatc gaagcagtcc gcaaccagga agaaaggacg gttctccccg gcggccgcct
     7561 cgagcgcatc catggcatcg aggaacaccc tgggggcgaa ccaatcctct tcgcccttgc
     7621 ggtcctgcac gttggccagg tactggcggg ccttcttgcc ctccccgagg ataatgtaac
     7681 ggtcccgcat ctcctcttcg gagatagtta gggggtcctt gtaagggtcc ctctcctggc
     7741 cccggatcac gttgtaggtc ccgaagcccc ggcggaagtt catggggaac tgtacgtaag
     7801 tgtcggtcac caggtaggtg ccgaagccct ctttcgacag tatctccgcc accgtagtgt
     7861 gcccggaggg gatcggcttc catccgaagg agggtggtcg ggttggccag gtcctcatcc
     7921 ccgtgtggat ggcacgcctc accgggatgg tgggcatggc ttcggggtag gcccggttga
     7981 agagcatact ctcctcggcg agggcatcga gggtgggggt ctgtatccag tcgttgccgt
     8041 aggcgccgac gtgatccttc ctcagggtgt ctatgatcac cagtatcaca ttcgacccgg
     8101 tcccaccacc tggaaggtac tcctcgggca accgcggcac tctgtcggtg aggcctgcgc
     8161 acccggccgc gccgagcagg gtcgcgcccg cgccggcggc cctcaagaag tccctgcgcg
     8221 taaacgtcct cttcggtttc ttcctcactg caggcctact cccaaacctg taccacaaga
     8281 gccgcactct agcagctaat acgttgttca tcagcaccgg ccagaccgac gcagaaagga
     8341 agccgtcgcc ggctattgtg gctcaggcca tgttcgcctt gagggcgtcg gccagatgat
     8401 agacgggggt gagttcgccc ggattcttcc tctccatgta ctccacgaac tcctcctccg
     8461 tgtcgaagag catcttgggg ttgctctcct tctcttcctt caggatcgag tgctcgcggc
     8521 cgttgtagtc gtgtcccggg aagaccagca ggtcgtcggg gagggcgcgg taggtgtaat
     8581 agagcgtgtg gtactggcgg accgcgttgc cgttgggtag atccgtgcgc ccgcaggacc
     8641 ctattagcag ggcgtcggcg gtaaggatgc ggccaggcag catcacggag accaggtcct
     8701 tggcgtgtcc cggcgtgtgg atgaacttga tagaaacgtc gccgaccagg agacggtcgc
     8761 catcctcgac cctcatgtct acgcagctcg caggggcctt ctcgtgcata acgatcttcg
     8821 ctgtggtctt gagctttagt tccctggcgc cggagatgtg atcggcgtgc gtgtgggtgt
     8881 ccactatgta ggccggcttc agaccgtgct cgaagatgaa atcgagcatg ggctcgacca
     8941 tctccagttc cgggtcgaag atcgcggccg agcgggcgtc ggcgactatg tagcccaggc
     9001 atccgcggtc gacgcggaac tgctcgaaga ggtagtcctg gtcactctct ttcacggacg
     9061 ctcctccttc tcggcgccgc gctctcgatc ccttagacca cctcgatagg gtgcccgacg
     9121 acgccgtcgt agaggtatcc tagatcattc caccccacct cgtcaggctg gacattgccc
     9181 gcgttatcgg ttgccctgac tctgatctcg tgctcgccgg gcttcgcgtc ccactcgaag
     9241 ctccatcgcg cccacgcccc tggggtgttt tccccgaata gccgcgcctc acgccaggga
     9301 ccaccaccca ccgagtactc gacgcgggag atggcaccgt aacccgacca cgaccggccg
     9361 ccgagtacgt gcctaccctc ctgaagccgt gctggccatg ccaactctag ggcgctcttc
     9421 acgccccgct cctctacggg ctcccgagcc gcgccggact ccccgccggt gagcacgtac
     9481 ttgtcggtgt tccacggaga gaagagctgc ttctcggaga cgtagatgcg gccgagccac
     9541 ttcacgctcg ccacggcggc ccagccggag acgatcgcgc gcagcgggaa accgtggtcc
     9601 ggcgtcagca tcgccccgtt catgctgaag gccagcaacg tgccttcttc cacggctttc
     9661 tccaccggta gcggacgccg catcctcacc gcgtccaggc tctcgagcat caccccgtgg
     9721 gccgaagcct tgaggccggc ccgctccagt atctcccgca acatgacccc ggtccactcc
     9781 gccacgccga cggccccgag cccccaaggg gtgccctctg gccgtcgcgc ctgaagctcc
     9841 tcgaaaaaag cgcggccgtt gccggcgcac tccagggcct gcacgatgga tacctgctgc
     9901 atgctcgaca ggtcgtcgta cgtgagccgc agcggccgtt cgacgccagg cccctccacg
     9961 tgcagggtcc agaacccagg atctatgctc ggggtcgcgg cgtggttgcg gacgaagaag
    10021 ctttcgacgg gcgtcaggca gccctcttcg gccatcgcct cccaccgcat ctccgcgttg
    10081 tacgcgaggt cgccggtagg atctcccggc ttcgcctcgc cttcccgcaa atccacgaac
    10141 ctctccggcg gcagagactt gcggatgcca cgctccgtca cggaggcggc gccactcgca
    10201 ttcctgggta cggagacggt agtccggaag ctgtgcaagc cccggtctcc tttcggcatt
    10261 gaggtcatcc cttctaaagg cgttccacgg ccgcccaatc gaccgccgcc aggaccgcgg
    10321 ccgccgacag cacaaaccat ctctggaaat ccgagagcgg caggaagaac gagatcgtcg
    10381 cgagtcccac caggaacaca aggttcgccg tcagcatctt acgcagagtt ccaccctctc
    10441 gtgggggagg acgacgtgaa gtccgctgct tctctgtact tcttccaccg agtttctcga
    10501 ccaagatacc actcctaacg cttcgcacct taaggtttca ctttttccat gtacagagtg
    10561 caatacatac ccccggcgac ccgggcgtcg aggctgggac cttggacttc gcacgccttc
    10621 ttcagcctct cgaaggactt gatagcggga gtgccaccgg tgctgatcac ctcttcgaga
    10681 agcgaagggc tcctctcgtg aacttcctcg gtcaccccga tagcgtgcac ctctacttgc
    10741 ccggtagctt gcgcgttcac tcaggacagc ccgccggatc gtcgacgtat ctcgccttgg
    10801 cgaagatccg caattcctta caacgcaggc accaacacat aatcgatact tcgtccaacc
    10861 tgacctctag caactcttct tcgcacgcgg cgtggcggag ggcgaactcg gcaaagtccc
    10921 acaaccccga agagtgaccg gcgcggtcgg ccacgagcat cgttgggccc ttcatatccg
    10981 gaggactgcc cctctcgttt ccggaagcat cataggccgg cgcacgctcg cactttctcc
    11041 agaaagccca gaaccgccca tgttgcagct tccccatccc ctaagcctcc acgatgggtt
    11101 cgggttcgcg gatcctgaaa gccttaccag gtaactaccc aagcggtgag tcttccgggg
    11161 cacgtaccga cacagcaggc actcttctac gcccttgagt gtgatcgcct tatccaaacc
    11221 ttctctctct ctctctctcc ttttccccgc cctgggcacg ttacagcatt gcctattttg
    11281 tctatagttt tacttaataa tatcgcagaa tataccgggg gcttctgggt gtcaaggcga
    11341 tccgcacacg ttctcggcac ttggctttcg gtactgtacc cgcactctct gggcaggaga
    11401 gttgggagcg tgcatcgaac tacaagcacg ttcgattagc ggaagtcatc tcgttcatta
    11461 gctgacgggt cgggagcggc gtcacgatag cttttagcgg ttgcaaagcg gtaccggcgg
    11521 cccccttggg atgggttcga gggatcaaat gaccccagcc gcgcgtccaa ggcggcttga
    11581 acgcgcggct ggtcgcagtc ttctcaggtg cggattctca cgcggtcttt ctgaccgccc
    11641 tccacttctc taccgcctcg tagcgggggg ccaggtactc ttccggcgtg cgtacgcctt
    11701 cgaggaagga gagggcctgc tcggcgagca gtgcgccttc gccgctagta gcccacgcgt
    11761 tctccctttc ttgggggtcg cgggcgaggt cgtagagctc gggctcgtcg tcgggtccgc
    11821 ccaggatcag ggaccgctcc ggcgtggtca cggtgagcgg catgtagtcg tcgatcctgc
    11881 gcgccttgga atccaccgcg gtgatgatct cacccttcgc aagatacaac ggccaggaac
    11941 tgaccacgag gggcctatgc tcatccctgg tcccgtcgag gacacccccg aaggagtcac
    12001 cggtcatcgt ggtcggtacg cctcccaggc ccacgaggtc catgatcgtc ggcgcaatgt
    12061 cgggggcggt cgtcatggcc tgcgtacgac ccggctcgag tccaggcccc cgcacaatca
    12121 ggggaatgtt ggtgagctcc ttgtagagcg gcgaccgctc gaccgtgagc agccaggaat
    12181 cctgcagcca ctctggaagc gaagagtctt tgctgacctc ggcctccggg tcgtgtaccc
    12241 actcggcctt gccgaagtag tcatgctccc cgaagtagaa gccgtggtcg gaggtgaaga
    12301 gaacgagcgt gttctccctg agcccgaggg cgtccagctt cgcgagcaag cgcccgatcc
    12361 agaagtcgac catcgtgact tcgccgcagt aggtggcatg tcccaggtcg acatcgtcct
    12421 ttttcagacc cgccttcttc cagttgccgt aggcggggta gatctgctcc ccgtcgtaac
    12481 cctcccggta cttcctggtg tagtagtctg gcgcgtccca cggctcgtgc gggtcccacg
    12541 tgtccacgta gaggaagaac tgctcgtcgt ggtgccgctc cagccaccgc tcggcctgcg
    12601 ctaccgtgcg cgcgaccatc ctgtcctcct caccacgcca cgtcgaacgc gcgtcggccc
    12661 gctcgtgcgg cctggtgtcg tcgccctgca cccgacccca cacgaagtcg tcgaagcctc
    12721 ggtcgtagcc gaaaccgttg cggatgtaga agggcgtgtc cacgacgccc atcgtcgtgt
    12781 a
//
LOCUS       DATMVF010000076         8252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_650227, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000076 DATMVF010000000
VERSION     DATMVF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650227"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VN178_03285"
     CDS             complement(<1..334)
                     /locus_tag="VN178_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80022.1"
                     /translation="MISKELLSIVRPRPSDTGGLLYAGGIESDGMILGGKARSPATGE
                     TYEVKDGYLDLLKSRTGADSLANLTNFLPGAGRGYEPLWRVRSLSLLTGESFPNEREL
                     EIIHRLVRP"
     gene            complement(335..961)
                     /locus_tag="VN178_03290"
     CDS             complement(335..961)
                     /locus_tag="VN178_03290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWS80023.1"
                     /translation="MPPDGKDEREYHWEVREADADALRSAMSHFPTGVTVVTSGREEK
                     VEGMTANAVISVSLDPLLFLVSVHKDARINQRIREEGSFTVNLLADDQEGLSRLFASP
                     ERSSGLPALHSLGGGYGSVGAPLAAGALAAIDCELAEIYAGGDHDLFLGRVVEVRLGD
                     TRKGPLIFHEGAYPTLKSAVRPEETGAFVDSVRGFDARLNRLRDRRRR"
     gene            complement(988..1707)
                     /locus_tag="VN178_03295"
     CDS             complement(988..1707)
                     /locus_tag="VN178_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80024.1"
                     /translation="MSGAPIVIVGGATWWPGSYRNFASLLREISGSEVHIAPLTPLDW
                     TLGYFRGFGQLVFEVASAVDRALLEGEPKKAVLVGHSAGGLACRVYIGGDPPYGGRRY
                     SGHRRVQSLITLGTPHTVADRPRLAPITRVNELFPGALHAEAGLRYLSVAGRVADGAS
                     SPRVRKRYERFVEDGRVAGDGIVPVDAALLPGSESQVLDGLHHNRRLGRWYGSDAETV
                     GRWWPQELRAGDTLVGDARAR"
     gene            complement(1855..2607)
                     /locus_tag="VN178_03300"
     CDS             complement(1855..2607)
                     /locus_tag="VN178_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80025.1"
                     /translation="MEILVTGLFDDPEDGFGLQQAVLEEVAAHRRGPTALMWTSSRYV
                     GATRPETRLPGFAAATEAASGLGFPVLVRNSGGGAVAANRGSLSFSLTFPVEDLRHGL
                     YERYSEGLDLIASALQRVGIEAEGGEVEGEFCPGAYSVRSGGPRGVKHAGLAQRVTRR
                     SARLEALVLVSETDEIRTVLERVYGLLGLPFRPESIADLPADISQLVQALTEEVRGRY
                     EAEKGRIGEMTMDRARSLRGGWRAIPDKSSSL"
     gene            complement(2612..3436)
                     /locus_tag="VN178_03305"
     CDS             complement(2612..3436)
                     /locus_tag="VN178_03305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS80026.1"
                     /translation="MTYSDPEYVMRQYRDASNLEARIALHERFSTNPYGLPRWILDQF
                     DLQNEASILEVGCGPGQLWTGNLDLLPKNWAITLTDASPGMVAEAEARLEPDGRFEFR
                     VADVRELPFRDGRFDAVVANHMLYHVPDRGRAFSEITRVLKPDGNLYAATNGEGTQGE
                     MGWMQQILDRSRPTDGYYFGNHLEFSLENGAEQLSPFFSEVTLRRYEDALHITEVRPL
                     VDYLLSGSAADTAARELDADEFDRRVTELTGRLEKELTSRGAIHIAKDTGLFIARK"
     gene            3528..4361
                     /locus_tag="VN178_03310"
     CDS             3528..4361
                     /locus_tag="VN178_03310"
                     /inference="COORDINATES: protein motif:HMM:NF044469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80027.1"
                     /translation="MLRQEAMIARTRNVCREDERLKAAMMYGSFAQGEGDGFSDIEFI
                     LFFRDEELEGLDQEEWVSRISPVAVYFVNEYGNGTAIFDNLVRGEFHFDRASEMESVV
                     GESMRDTDWLPSLEDTLILDRTGELALRLRKIVGPPPQRDTPGQIRFVCDSFVNWFIF
                     GSNLFARGELARSLDLLGIVRDRLLQMVRVLEGSTLHWFNSAKFLETEISEASYVRYA
                     ACTARLDGEELRQAYASAWEWGRELMASLGKRHGVVPPTPLLERLDLRFAETFGGPEG
                     P"
     gene            4358..4921
                     /locus_tag="VN178_03315"
     CDS             4358..4921
                     /locus_tag="VN178_03315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWS80028.1"
                     /translation="MRSEETQNPWRTLGSRDVYENPWISVREDRVIRPDGEPGIYGVV
                     HYKNTAVGVLPVEEDHVYLVGQYRYPLERYSWEIPEGGCPEDEEPLRAARRELREETG
                     LEARNWRKLGEAYLSNSVANERAVWYLATGLVPGERQPEGTEVIGVRRVPLREAVAMA
                     RDGRITDALSILALTTYVLEADDVVAG"
     gene            4949..5890
                     /gene="cofD"
                     /locus_tag="VN178_03320"
     CDS             4949..5890
                     /gene="cofD"
                     /locus_tag="VN178_03320"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HWS80029.1"
                     /translation="MKVCALAGGVGGAKLASGLRDVLPTGDLSVVVNTADDFDLWGLH
                     ICPDLDTVMYTLAGISNPDTGWGIADETFETLDMLEHYGEEIWFKLGDRDLATHILRT
                     SRMRSGETLTEVTAGLSVALGVESAVLPMSDDPVSTVLETPEGRLEFQEYFVRRGQRD
                     EVLGIELRGIEEARPTERVLATISGADAIVLCPSNPVVSVGPILALPGMTEALASSTA
                     PKVAVSPIVGGRALKGPADRMLASLGHEVSATGVARMYAGLVEGMVVDRTDRGERAGI
                     EALGMRVLVTESVMRGVEDRARLASETLEFGAGLVAR"
     gene            5887..6501
                     /gene="cofC"
                     /locus_tag="VN178_03325"
     CDS             5887..6501
                     /gene="cofC"
                     /locus_tag="VN178_03325"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HWS80030.1"
                     /translation="MSRTRDVSAVVPVKDLRGTKSRLAPILDPGARAGLTLYMMGRVV
                     RAILEAGIEDVCVVSPDRIVLEEAKRRGATPLVQESRGLNPALEEGRRRALGLGASTL
                     LVLPADLPLLDAGDVRAVLQETGEGSSAVISPDGTRSGTNALLIQPPGLLPFAFGADS
                     FEAHLGAARQRGLDVRICDRPHLAFDLDTVGDLARLKETGVARS"
     gene            6498..7241
                     /gene="cofE"
                     /locus_tag="VN178_03330"
     CDS             6498..7241
                     /gene="cofE"
                     /locus_tag="VN178_03330"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HWS80031.1"
                     /translation="MRGVEVLGVEGFPEISPGEDLNRMISDAVARDLRPGDVLVVTHK
                     IVSKAEDRLVDLRTVEPSALAKGYAMRYGKDPRQIEVVFRESRRIVRMDRGIVISETH
                     HGFVCANAGVDASNVPGDDTVCLLPVDPDASAARLRDALAEDPGVEVAVVISDSFGRA
                     WRHGIADVAIGVAGMDPVADYRGRRDPHGYPMEASVLAVADELAAAAELVMGKTDGIP
                     LAIVRGYSYSPAEGSARELLMPPDRDMFR"
     gene            7424..>8252
                     /locus_tag="VN178_03335"
     CDS             7424..>8252
                     /locus_tag="VN178_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80032.1"
                     /translation="MNNLRAYPIYLGIRGSFAFFFTLWATVAAVYRIEVVHLDPLRLV
                     LLGTALEVAVFIFEVPTGVFADTYGRRRSVITGCVLMGCGFALEGAIPEFITVLAAQA
                     IWGVGYTFISGALEAWIADEAPERDLGRVYLRGEQADYIGAFAGIPASVLLGLVALNL
                     PLIVGGVLTIALGLVLILTMPERNFRPSPREGRSSLQHAATTARSGVRLVRSRPVLLT
                     LLAAALFAGMSAEGFDRLNPKQFLDVVELPSVGGLDPVVWFGVIGAGGLFLSYLTAGI
                     "
BASE COUNT         1403 a         2636 c         2736 g         1477 t
ORIGIN      
        1 cgggacggac gagacgatgg atgatctcca gctcccgctc gttcgggaag gattcgccgg
       61 tgagcaggct gagggagcgc acgcgccaga gcggttcgta gccgcgtccg gcgccaggca
      121 ggaagttcgt gaggttggcg aggctgtccg cgccggtgcg ggatttcagg agatcaagat
      181 atccgtcttt gacctcgtag gtttcgcccg tcgccggaga gcgggctttg ccgcccagga
      241 tcataccgtc actctcgatg ccaccggcgt agaggagacc tccggtatcc gaaggacggg
      301 gacggacgat ggagagcaac tccttgctta tcatctagcg ccgtcttctg tcgcgcaggc
      361 ggttcaggcg ggcgtcgaag cccctgacgg agtcgacgaa ggcgcccgtt tcctcgggac
      421 ggacggcgga tttcagggtc gggtaggcgc cctcgtggaa gatcagaggt cccttgcgcg
      481 tatcacccag acgcacctcg acgaccctgc cgaggaacag gtcgtggtcg cctcccgcgt
      541 agatctctgc cagctcgcag tcgatcgccg cgagagcacc ggccgcaagc ggcgccccca
      601 ccgagccgta tccgccgccc aaagagtgga gcgccggtag tccgctggag cgttctggcg
      661 aggcgaagag cctgctcagc ccctcctggt cgtccgccag taggttcacc gtaaaggagc
      721 cttcctccct gatacgctgg ttgatacgag cgtctttgtg gacgctcacc aggaacagca
      781 gcggatcgag ggagacggag atcacggcgt tcgcggtcat gccttcgacc ttctcctccc
      841 tcccgctagt tacgaccgtc acacctgtag ggaagtggct catcgcgctc cgcagggcgt
      901 cggcgtcggc ctccctgacc tcccagtgat actctcgctc gtccttaccg tcaggtggca
      961 tccacacctc ttctggccag ccatacccta cctggcacgc gcgtctccca cgagagtgtc
     1021 tcccgcgcgc aactcctgcg gccaccatcg ccccaccgtc tccgcgtccg agccgtacca
     1081 acggccgaga cgcctgttgt gatggaggcc gtcaagaacc tggctctccg agccgggaag
     1141 cagggccgcg tcgacgggca cgatcccgtc tccggccacc ctaccgtctt cgacgaaccg
     1201 ctcgtagcgt ttacgtaccc tcggtgagga ggcgccgtcg gcgaccctac cggcgaccga
     1261 gaggtagcgc aagccagctt cagcgtggag ggcgccggga aagagctcgt tcacgcgggt
     1321 tatcggagcg aggcgcggtc tatctgcgac ggtgtgcggg gtgcccagtg tgatcaggga
     1381 ctgcaccctc ctgtgtcccg agtaacgcct gcctccatat ggaggatctc cgcctatata
     1441 caccctgcac gcgagaccgc cagccgagtg tcccacgagc accgcctttt tcggctcgcc
     1501 ctcgagcagc gccctgtcca cagcgctcgc gacctcgaag accagctgcc cgaagccccg
     1561 gaagtatccc agggtccagt caaggggtgt aaggggggca atgtgcactt ctgagccgga
     1621 gatctcccgc aacagacttg cgaagttgcg gtaactgccc ggccaccagg tggccccgcc
     1681 gacgatcact atgggtgcgc ccgacatgcc cgtgagtata gccgccttca ggtgatgctc
     1741 gcctggaggc cgtcaatcac gaactggacg atgggggcga gacccggcgg gtccacgacc
     1801 acgaacagcg tcacgaaagc gttgaacgcg atctccatgg gaaggaactt tacttcacag
     1861 cgaagaactc ttgtcaggga tagcacgcca cccaccccgc aaagaccgcg cccggtccat
     1921 ggtcatctct ccgatccgtc ctttctccgc ctcgtaacgc ccgcgcactt cctcagtgag
     1981 cgcctgtacc aactgcgaaa tatctgccgg caggtccgcg atgctctctg gccggaacgg
     2041 cagtccgagg agaccgtaga cgcgttccag tacggtcctg atttcatccg tctcggagac
     2101 cagaacgagg gcttcgaggc gcgcggaacg gcgcgtgacc ctctgggcga ggccggcgtg
     2161 cttgacgccc cgcgggccgc cggaacgcac ggagtaagcc ccggggcaga actcgccttc
     2221 gacttcgccg ccctccgctt cgattccgac tcgctgcagt gccgaggcga tgagatccag
     2281 gccctccgag taacgttcgt agaggccgtg ccgcagatcc tctacgggaa aagtgagaga
     2341 gaacgacaaa gaacccctgt tggccgccac tgccccaccc ccagagttcc gcaccagcac
     2401 gggaaagccg agtcctgagg cggcctctgt cgccgccgca aagccgggca gacgggtctc
     2461 cggccgggtt gccccgacgt agcgcgaaga ggtccacatc agggctgtgg ggccgcgtcg
     2521 gtgcgccgcg acctcctcga gcacggcctg ttgcagcccg aagccgtctt cggggtcgtc
     2581 gaatagccct gtgaccagga tctccacgcg cctatttgcg ggcgatgaaa agccccgtgt
     2641 ctttggcgat gtggatggct ccccgcgagg tcaactcctt ttccagtcgc ccggtcagct
     2701 ccgtcaccct ccggtcgaac tcgtcggcgt cgagttcccg cgccgcagtg tctgccgccg
     2761 agccagacaa caggtaatcg accagcggcc ttacttcggt gatgtggagc gcgtcttcgt
     2821 atcttcgtag cgtgacctcc gaaaagaagg gcgacaactg ctcggcaccg ttctccaggc
     2881 tgaactcgag atgattccca aagtagtaac cgtcagtcgg ccgagatcgg tcgagaattt
     2941 gttgcatcca acccatctcg ccctgcgtgc cctcgccgtt agtcgcagcg tacaggttgc
     3001 catctggttt gagaacgcgg gtgatctccg agaatgcccg ccctctgtcc ggcacgtgat
     3061 agagcatgtg gttcgcgaca acggcgtcga atctcccgtc ccggaaaggt agttcccgga
     3121 cgtccgcaac ccggaactcg aagcgtccgt cgggctcgag ccgggcttcg gcctcggcca
     3181 ccatcccggg cgaggcgtcc gtcagcgtga tggcccagtt tttggggagg agatccaggt
     3241 tcccggtcca caactgtccg ggaccacaac cgacttccag aatgctggcc tcgttttgta
     3301 gatcgaattg gtcgaggatc caacgcggta ggccgtaggg gttggtgctg aaccgttcgt
     3361 gcaacgcgat gcgggcttcg aggttcgagg cgtcgcggta ctgcctcatt acgtattccg
     3421 ggtccgagta cgtcacgctt tgcctcctgg ttgggtacct ggcgggccgc gacagctatg
     3481 gtgctaagat agcagcccgg acgcagggtc ggagagtacg gaggacgatg ctcaggcagg
     3541 aagccatgat cgcacgcacg cgcaacgtct gcagggagga cgaacgtctc aaggcggcga
     3601 tgatgtacgg ctccttcgcc cagggcgagg gggatggatt ctcggacatc gagttcatct
     3661 tgtttttcag ggatgaggag ctggagggtc tcgaccagga ggaatgggtc tccaggatct
     3721 cgcccgtcgc ggtgtacttc gtcaacgagt acgggaacgg aacggcgatc ttcgataatc
     3781 tcgtcagggg cgagttccac ttcgatcgtg cctcggagat ggagagcgtc gtcggcgagt
     3841 cgatgaggga cacagactgg cttccctcgc tggaagatac cctcatcctc gaccgcaccg
     3901 gggagttggc cctacgcctg cgaaaaatcg tcggtccgcc gccgcagcgc gacacgccgg
     3961 ggcagatccg gttcgtctgc gacagtttcg tgaactggtt tatcttcggc tcgaacctct
     4021 tcgccagggg cgagctcgcg cgttcgctcg acctcctcgg gatagtacgc gaccgcctgc
     4081 tgcagatggt cagggtgctg gaagggtcga cattgcactg gttcaactcc gcgaagttcc
     4141 tggagacgga gatctccgaa gcgtcctacg tccgctacgc ggcctgcacg gcccgcctcg
     4201 atggggaaga gctgcggcag gcttacgcct cggcctggga gtgggggaga gagcttatgg
     4261 catcgctcgg aaagaggcac ggcgttgtcc cgccgacgcc actcctcgag cggctcgacc
     4321 tgcgcttcgc cgagaccttc ggcggtccgg agggaccgtg aggtcggagg agactcagaa
     4381 cccgtggcgg actctcggtt cgagggacgt ctacgagaac ccctggatct cggtccgcga
     4441 ggaccgcgtg atccggcccg acggcgaacc tggcatctac ggcgtcgtcc actacaagaa
     4501 caccgccgtc ggcgttctgc ccgtggagga ggatcacgtc taccttgttg gccagtacag
     4561 atatcccctg gaacgctact cctgggagat acccgagggc ggctgcccgg aggacgaaga
     4621 acctctgcgc gcggccaggc gcgagctgag ggaggagacg ggcctcgaag ccaggaactg
     4681 gcgtaagcta ggggaagctt acctttccaa ttctgtcgcc aacgagcgtg ctgtatggta
     4741 cctcgctacg ggtctcgtac caggggagcg tcaaccagaa gggaccgaag tcataggcgt
     4801 tcgacgcgtc cccttgcggg aggccgtggc catggcgagg gacggcagga tcaccgatgc
     4861 tctcagcatc ctcgcgctaa cgacctacgt actcgaggct gacgatgtcg tggccggcta
     4921 gggcaggccg ccgcgtaccg ggggcgcggt gaaagtctgt gcgctcgccg ggggagtggg
     4981 cggcgcgaag ctcgctagcg gtctgcgaga cgtgctgccg accggggatc tctcggtggt
     5041 ggtgaacacc gccgacgact tcgatctgtg gggcctgcac atctgccccg atctcgacac
     5101 ggtcatgtac acgctggccg gtatctccaa ccccgacacc ggttggggca tcgcagacga
     5161 aacgttcgag accctggaca tgctggagca ctacggcgag gagatctggt tcaagctcgg
     5221 agatagggat ctcgcgaccc acatactcag aacgtcccgc atgcgctccg gggagactct
     5281 cacggaggtt acggccgggc tatccgttgc tctgggcgtg gagagcgcgg tcttgccgat
     5341 gagcgacgat cctgtttcga cggtgctaga gacccccgaa gggcgcctcg agttccagga
     5401 gtacttcgtg cgccgcgggc agagagacga ggtgttgggg atcgagttgc gcggtatcga
     5461 ggaggccagg cccacagaaa gggttcttgc gacgatcagc ggtgccgacg ccatcgtctt
     5521 atgtccgtcg aacccggtag tgagcgtagg cccgatcctt gccctgccgg gaatgacgga
     5581 ggccctggcc tcgtccaccg ccccgaaggt ggccgtcagc ccgatcgtcg gcggacgggc
     5641 gctcaagggt ccggcggaca ggatgctcgc ttcgctcggc cacgaggtct ctgccacggg
     5701 cgtggccagg atgtatgcgg gcctggtcga ggggatggtc gtcgaccgga ccgaccgggg
     5761 tgaacgggcc ggcatcgagg ccctcgggat gcgtgtgctg gtaacggagt cggtcatgcg
     5821 cggcgtcgaa gaccgggcgc gactggcgtc ggagacgctg gagttcggtg cgggattggt
     5881 ggcgcgatga gtaggaccag ggacgtatcc gccgtcgtgc ccgtcaaaga tttgcggggc
     5941 accaagagtc gtctggcgcc gattctagat cccggcgcca gggcagggct aaccctgtac
     6001 atgatgggcc gcgtagtgcg cgcgatcttg gaggcgggga tcgaggacgt ttgcgtggtc
     6061 agccccgacc gaattgtcct ggaagaggcg aagagacgcg gcgcaacacc gctcgtccag
     6121 gaaagccgcg ggctcaatcc cgccctcgaa gagggccgcc gcagggcgtt gggactcggt
     6181 gcctcgacgc tcctcgtgct ccccgcagac cttccgctcc tcgatgcggg tgatgttcgg
     6241 gcggtgctgc aggagaccgg tgaagggtct tcggcggtta tctcccccga cgggacgcgt
     6301 tccggtacga acgcgctcct aatacaaccc cccggtctcc tgcccttcgc cttcggggcg
     6361 gacagcttcg aggcacacct tggggctgcc cgccagcgcg gcctggacgt caggatctgc
     6421 gaccgacccc acctggcctt cgatctcgat acggtgggag acctggcccg cctcaaagag
     6481 acaggagtag cgcgctcgtg aggggggtcg aggtgctcgg cgtcgagggc ttccccgaga
     6541 tcagccccgg cgaagacctg aaccgtatga tctcggacgc ggtagcgagg gacctgcgtc
     6601 ccggcgacgt actcgtcgtg acgcacaaga tcgtcagcaa ggccgaagac cgtctggtcg
     6661 accttcgcac ggtcgagcct tccgcgctcg ccaagggtta cgcgatgcgc tacgggaagg
     6721 acccgcgcca gatcgaggtc gtgttcaggg agagccgccg catcgtacgc atggaccgtg
     6781 gcatcgtcat ctcggagacc catcacggtt tcgtctgcgc caacgcgggc gtagacgcct
     6841 cgaacgttcc tggcgacgac accgtgtgcc tgctgcccgt agatcccgat gcctctgcgg
     6901 cgaggctccg cgacgcgctc gccgaagacc cgggagtcga ggtggccgtg gtgatctcgg
     6961 actcgttcgg gcgggcctgg cggcacggca tagcggacgt cgccatcggt gttgccggta
     7021 tggaccccgt cgccgactac agggggcggc gagacccgca cggctacccc atggaggcca
     7081 gcgtactcgc cgtggccgac gagctcgccg ccgccgctga gctggtgatg ggcaagaccg
     7141 acggaatccc tctggccata gtgcgggggt attcgtactc gcccgcagag ggcagcgcca
     7201 gagagctcct catgccgccc gatcgggata tgtttcgctg attcagcatt tcagcacgcc
     7261 gccaggcttc gccaggcggc ttgtcagcac ttcagcccgg cggctgcgcc gcctcgtcag
     7321 ccggcaaggg ttcgctcgaa ggctgaaatg ctgactggct gaaatgctga ctagcgaagg
     7381 cgaagcctgg cggcgtgctg gtattcgaga aagcaggact tgagtgaaca acctgagagc
     7441 gtatccgatc tacctcggta tcagggggtc gttcgcgttt ttcttcacgc tttgggccac
     7501 cgtggcggcc gtctaccgga tcgaggtcgt tcatctggac ccactgcggc tcgtgttgct
     7561 ggggacggcg ctcgaggtcg ctgtcttcat cttcgaggtt cccaccggcg tgttcgccga
     7621 cacttacggc cggcgacggt cggtcattac gggttgcgta ctgatggggt gcggtttcgc
     7681 gctggagggt gccatccccg aattcataac cgtgctcgcc gcccaggcga tttggggtgt
     7741 ggggtacacg ttcatcagcg gggctctgga ggcctggatc gcggacgagg ccccagaaag
     7801 ggatctggga agggtatacc tgcgtggaga gcaggcggac tacatcggcg ccttcgccgg
     7861 catccccgcc agcgtgcttc tcggtctcgt cgcgctgaac ctgccgctca tcgtcggcgg
     7921 cgtcctcacc atagccctgg gcctcgtgct catcctcacg atgcccgaac gcaacttccg
     7981 accctccccg cgcgagggcc gctcctcgtt gcagcacgcg gccaccacgg cgcgcagcgg
     8041 cgtgcggctc gtccgttcca ggcccgtcct cttgacgttg ctcgccgccg ccctgttcgc
     8101 cggcatgtcc gcggaaggtt tcgaccggct caaccccaag cagttcctgg acgtcgtgga
     8161 attgccttcc gtcggcggtc tcgaccccgt ggtctggttc ggtgtaatcg gggccggggg
     8221 cctcttcctt agctacctga cggccgggat cg
//
LOCUS       DATMVF010000077        11376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_7583, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000077 DATMVF010000000
VERSION     DATMVF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7583"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1842
                     /gene="cydC"
                     /locus_tag="VN178_03340"
     CDS             106..1842
                     /gene="cydC"
                     /locus_tag="VN178_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HWS80033.1"
                     /translation="MRVFLRLLVFMRPYKWRILLGVLLGAATVASNVGLLATAAYVIS
                     AAAVVPFLAMLTIPVYLVRLFSVSRAASRYAERLVSHDVTFKLLAELRTRFYARLAPL
                     TPASLLRYRSGDLLSRIVKDGEELQNVYLRSFSPVAVALAVCTLAALVLYTFDPTMTF
                     VAIAFLAATGVGVPVLVRALSKGLGQRQLQLRAELEARIVDDVQGVQDVLAFGREGGE
                     QHEVSILNGKLGRVQRRMALVTGLQNALSDLMMNLALVTVLIVTIPLVAAGKMDGVFL
                     AFIALIVLGSFEAVAPLGAAFQVLGRSVGAGERLFEITDSEPLVTDPCEPLSLPQDGT
                     LALDRVSFRYEEEGPPAVRDVSFVLRPGKRVAVVGPSGSGKSTLVGLILRFWDPESGE
                     VRLGGWDVRRYAQEDLRSRIGVVSQDAHVFNDTMRNNLLLADPEADDVALHRAITEAH
                     LVDLVEGLPGGLDGYVGEQGQRLSGGERQRLAVARALLKDAPLLLLDEATADLDTVTE
                     RELLGTIRELMRGRTTLRITHRLVEMEMMDEILMLDEGRVVERGTHEELVQRDGPYRR
                     MLAVQDDMLATG"
     gene            1948..3297
                     /locus_tag="VN178_03345"
     CDS             1948..3297
                     /locus_tag="VN178_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWS80034.1"
                     /translation="MEDALLVSRLQFAFTVTYHYLFPMLTMGLALLIFVLKSAYMRNR
                     NELYNDSARFWGKIFAVTFVMGVVTGIPLEFQFGTNWAAFSAYAGDIIAQTLAMEGAF
                     AFFLESAFVGLFLWGEDSFGQRVHWFSSLMVFLGTWASGYFIIATNAWVQHPVGYRVL
                     ENGNIELDNYWAVLLNSWMWPQYAHNMGGAVVCGAFVMAGLGAFYVLGRRHEEYGRIF
                     LKVGVIAGVLASLWMLFPSGHFSSEQVAEHQPVSLAAMEGQFETERPAGIVFIGQPDV
                     ENRRIDNPIVLPRALSFLIYQNWNAEVKGLEAFPEKNWPDNIPLLYYSYHVMVGLGTI
                     FIAIMVVSAFLLWRGRLYGSRWMLWVLMLAIPFPFIANTAGWFTAELGRQPWLAYGLF
                     RTSEGVSPLVSSGSVLFTLIGFAGMYLIMGLLYIVLMVREVATAREAEEMFSSHPRV"
     gene            3315..4340
                     /gene="cydB"
                     /locus_tag="VN178_03350"
     CDS             3315..4340
                     /gene="cydB"
                     /locus_tag="VN178_03350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWS80035.1"
                     /translation="METIWFIFVALMVTMYVLLDGFDLGAGAIHLFAARNDRERRTII
                     RAIGPVWDGNEVWLIAAGGTLFFAFPTLYASGFSGFYLPLIIVLWLLMIRGLSIELRG
                     HISDPMWASFWDAAFFVGSSLLAIFYGAALGNVVRGVPLDESGYFFQPLWTDFSPFSG
                     TPGILDWYTVLIGLLALTTLVVHGSNFIAVKTEDDLNARSRRIARRFSFATILLTLFA
                     TPATFWVSPEVLWSFNERPYGYVLPLVAVAGLAGMVYFDLRGRDREAFLSSSAYIVGM
                     LTSTVFGVYPKVLPAVDPANSLTVQNASASTYGMAVGLVWWSIGMVLAVVYFTVIYRL
                     FRGKVRL"
     gene            4602..4973
                     /locus_tag="VN178_03355"
     CDS             4602..4973
                     /locus_tag="VN178_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80036.1"
                     /translation="MFYETTHAWKGSSMRSPWWLIARGPTDHMEVFTLDGGSILPVFS
                     GEDEAEMYLWFEEACDDGWEVRRSSPEELMSVLYGPCSDAGAVALDPSPEIIDGPSAW
                     LVSLNRRRFLGWMVSGRIPVS"
     gene            5724..6278
                     /locus_tag="VN178_03360"
     CDS             5724..6278
                     /locus_tag="VN178_03360"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWS80037.1"
                     /translation="MTKDYEQSQTIDAPPEEVFAWLADVDNLPRYLPPVVSSSAEGPS
                     AEGKPGRRVRTTLEYPGGEGTFDAQGYLAFDELERRMEWGAEAGRDYSGWLKVGSHGQ
                     GGSEVVVHLSFGERSVEGEIQSESPEGQDPLTEGILATLESIRRQIEEGAGKVQPPRS
                     GVEPDTGENPAVVRDDPPSGAPGS"
     gene            6557..7030
                     /locus_tag="VN178_03365"
     CDS             6557..7030
                     /locus_tag="VN178_03365"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWS80038.1"
                     /translation="MPQRVQESVEVQAPLQEVFDYWSNFEHFPEIMSNVEEVRVIDRE
                     RSHWKVKGPVGTSVEFDARTTEMDPARGIGWNSVEGEVDTSGEVRFEEVASDRTRIEV
                     TMNYADPPGGKVGEVVADAISNPERMMREDLENFAARVERGEVEFAGPGSQAQGR"
     gene            complement(7206..8384)
                     /locus_tag="VN178_03370"
     CDS             complement(7206..8384)
                     /locus_tag="VN178_03370"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWS80039.1"
                     /translation="MKVVGIISGTSFDAIETAAADLRLEGDAVVLRPLGSRGVPYDTG
                     LRTAVAEALPPAATTAGGVCMLDTRLGRAFAEAAADATLAFCGGHADLIVSHGQTVFH
                     WVEGEKALGTLQLGQPAWIADRTGSPVVSDLRSRDIAAGGQGAPLASIFDVLLLGSGE
                     RRRAALNLGGIANITVVPDGEAGDTPFAFDVGPANALLDAAVEYLTDGSETYDHEGRR
                     AARGGIHEGLLRSLLAEPYYGLGPPKSTGKELFNLPYLLERLAPFGDIEADDLVATVT
                     ALTSRTVADALERYDILEVVASGGGTSNPTLMGMLREDAKEVRLRRIEDWGIPSGAKE
                     AYAFALMGFLSVHGLPSNVPSCTGARSAVVLGSVTPGRSPLVLPDPAPRAPDHLRIED
                     "
     gene            8624..9988
                     /locus_tag="VN178_03375"
     CDS             8624..9988
                     /locus_tag="VN178_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80040.1"
                     /translation="MASVSEGRGGSPLRSTGEALRALSAGCVGNFVEWYDFAIYSYSV
                     PIIATVFFPEGNRTAAILSAFALYGVAFLARPLGGVFWGNLGDRVGRRNTLAAIVLLM
                     GGGTMLIGLLPTYAQVGLLAPTLLALLRLVQGFSGGGEFTGSTSFITEYAPEGSRGLY
                     AAISATFTTLPSLLGGLTVLAFTAVMSDAAYASWGWRIPFLVGGPLALVGLWIRLRME
                     ETPAFRRVQSTEHVEGVPVREAIRHHSREIVLVFAIASLSALGAYTLGAYFVTYLQEV
                     VGLDPTTAILANFTAFFVTVPLVPLVGALGDRIGRKPLLFIGSVGFVVLSVPGYLLAG
                     SGGLVTAILGQLLVALPWSFVVSAVVVTQVEIFPTRVRYSGASVGYNVAYMIFGGTAP
                     LVATFLVARTGSDIAPGVYLVAVALVVFFVAFAVPETYRFSLLREGEERREGSLDATG
                     ARPS"
     gene            10020..10937
                     /gene="murQ"
                     /locus_tag="VN178_03380"
     CDS             10020..10937
                     /gene="murQ"
                     /locus_tag="VN178_03380"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HWS80041.1"
                     /translation="MAKRPVITEQRNQASAGLDEMPVESILYLMNQEDRRVPEAVAEA
                     LPQIAEAVGLMVEAWRSGGRWIYIGAGTSGRIAALDAAECQPTFGVPQGRVVALIAGG
                     EAALTRSVEGAEDDRAAAVKDLEDSSLEPGDVVVGIAASGRTPYVVAGLRYASGMGCP
                     TIGISNNPGSELGAAADVCIELITGAEVLTGSTRLKAGTSEKLVLNMLSTAAFVRLDK
                     VYENFMVDLQAGNEKLKERSRRIVWEAAGVTRDEAGELLRAAGGSVKVAVVMGKAGVP
                     ADAARELLAAAGGNVRRALDAATPPDREW"
     gene            10955..>11376
                     /locus_tag="VN178_03385"
     CDS             10955..>11376
                     /locus_tag="VN178_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80042.1"
                     /translation="MSRYILIDGGQGGCRIVYLADGKQILTGSGAGLGRRARDRSAGV
                     LGVLERAFADLGPRAPDAVDAIVAGLTGFDGASETAGAVADGVRKLVRSGRVVVTNDA
                     VTSYLGAIGFEPGAVVAAGTGVIALAGDRDGNFARSDG"
BASE COUNT         1989 a         3528 c         3802 g         2057 t
ORIGIN      
        1 cgtcctccac gaagggcggg tcgtggagac cggaacccac gcaggactgc tcgaacgcaa
       61 agcgcattac tcgcttctgg ttggcgccta cgaggacatg ccggcgtgag ggtatttctg
      121 cgtctgctcg tcttcatgag accctacaag tggcggatcc ttctgggcgt cctgctgggg
      181 gcagcgacgg tggcgagcaa cgtggggctc ctggctacgg ctgcgtacgt gatctccgcc
      241 gccgcggtgg tgcctttcct ggccatgctc accatcccgg tctacctcgt ccggctcttc
      301 agtgtctcac gggccgcttc gcgttacgca gagaggctcg tctcgcacga cgttactttc
      361 aagctgctgg cggaactcag aacccgcttc tacgcccgcc tcgcgcccct cacgccggcc
      421 agcctcctga gataccgcag cggagatctt ctctcgcgca tcgtgaagga cggcgaggag
      481 ctgcagaacg tctacctgcg ctccttctcc cccgtggccg tggcgctcgc cgtctgcacg
      541 ctcgccgccc tcgtcctcta caccttcgac cccacgatga ctttcgtagc gatagccttc
      601 ctagcagcga ccggcgtcgg ggtgccggtg ctggtgcggg cgctctctaa aggactggga
      661 cagcggcagc tgcaactgcg ggcggagctc gaggccagga tcgtggacga cgtccagggg
      721 gtacaggacg tcctcgcttt cggccgggaa ggcggggagc agcacgaggt ctctatcttg
      781 aacggcaagc tcggccgggt gcagcgccgg atggccctgg tgaccggtct gcagaacgct
      841 ctcagtgacc tgatgatgaa cctggcgctg gtgacggtgc tgatcgtcac gatacccctc
      901 gtcgctgcag ggaagatgga cggtgtcttt ctagcgttca tcgcgctcat agtgctcggc
      961 agcttcgagg ccgtagcgcc gctcggtgct gccttccagg tcctcggccg ttccgtcgga
     1021 gccggcgaac gcctcttcga gatcaccgac tcagaaccct tggtcacgga cccgtgtgaa
     1081 cccctgtccc tgccacagga cggcacgctg gcgctggacc gggtgagctt ccgctatgaa
     1141 gaagagggcc cgccggcggt gcgggatgtc tcgttcgtat tgcggcccgg aaagcgggtc
     1201 gcggtcgtgg ggcctagcgg ttcgggcaag agcacgctgg taggtttgat cctgcgtttc
     1261 tgggatcccg aaagcggcga ggtacgactc ggtggatggg atgtccgccg ctatgcccag
     1321 gaagatctga gatccaggat cggggtcgtc tcccaagacg cccacgtctt caacgacacg
     1381 atgcgcaaca acctgcttct cgccgatccg gaggcggatg acgttgctct gcaccgggcg
     1441 ataacagagg ctcatctggt ggatctcgtg gaagggctgc ctggtggcct ggacgggtac
     1501 gtcggcgagc agggccagcg gctctccggt ggggagcggc agcggctggc cgtcgcccgg
     1561 gccctactca aagacgcccc gctgctcctg ctggatgagg ccacggccga tctggacacc
     1621 gttacggaac gcgagctcct cgggacgata agggagttga tgcggggcag gaccacgctc
     1681 cggatcaccc accggctcgt cgagatggag atgatggacg aaatactgat gctggacgag
     1741 ggacgcgtcg tcgagcgtgg cacccacgaa gagctggtcc aaagggacgg accgtaccgc
     1801 cggatgctcg ccgtgcagga cgacatgctg gcgacgggct gaacggaggg cggtttggcc
     1861 tgccacccac gaaattggtc agatttgatc aggcgccacc ccggcgggac ctgtagcctg
     1921 atccagaaat ccgcaggagg ttaggtcttg gaagacgcat tgttggtatc ccggttgcag
     1981 ttcgccttca cggtgaccta tcactacctc tttcccatgc tcaccatggg gttggcgctg
     2041 ctcatcttcg tcttgaagag cgcctacatg cgcaaccgga acgaactgta caacgactcg
     2101 gcgcggttct ggggcaagat cttcgcggta acgttcgtta tgggcgtggt gaccggcata
     2161 cccctcgagt tccagttcgg gaccaactgg gccgctttct ccgcgtacgc cggggacatc
     2221 atagcccaga ccctggccat ggagggtgcc ttcgccttct tcctggagtc cgcgttcgtg
     2281 ggtctgttcc tctggggtga ggacagtttc ggtcaaaggg tgcactggtt ctcgtcgctc
     2341 atggtcttcc tgggcacctg ggcctccggg tacttcatca tcgcgaccaa cgcctgggtg
     2401 cagcacccgg tcggctaccg ggtgctcgag aacggcaaca tagagctcga caactactgg
     2461 gcggtcctgc tcaactcctg gatgtggccc cagtacgcgc acaacatggg cggggccgtg
     2521 gtctgcgggg cgttcgttat ggccggcctg ggggccttct acgtgctggg acggcgccac
     2581 gaggagtacg gaagaatatt cctcaaggtc ggcgtgatag ccggcgtgct tgccagcctc
     2641 tggatgctct tcccgagtgg gcacttctcg agcgagcagg tcgccgagca ccagcccgtc
     2701 tcgctcgccg cgatggaggg tcagttcgag acggagaggc ccgcgggtat cgtgttcatc
     2761 ggccagcccg acgtagaaaa caggcgcatt gacaacccca tcgttctgcc gcgtgcgctc
     2821 agcttcctga tctaccagaa ctggaacgcc gaggtgaagg gactcgaagc cttccccgag
     2881 aagaactggc cggacaacat cccgctcctg tactacagct accatgtcat ggtgggcctc
     2941 ggcaccatct tcatcgcgat catggtcgtt tcggccttcc tgctgtggcg gggaaggctg
     3001 tacggctcgc gctggatgct ctgggtcctg atgctcgcca tacccttccc tttcatcgcg
     3061 aacacagcag ggtggttcac ggccgaactc ggccgccagc cgtggctagc ttacggtctg
     3121 ttccggacgt cggagggcgt atctcccctc gtctcctcgg gtagcgtgct cttcacgctc
     3181 atagggttcg cggggatgta cctgatcatg ggtctcctgt acatcgtact catggtgcgc
     3241 gaggtggcca cggcccggga ggcggaggag atgttctcga gtcacccgag agtctgacgg
     3301 cctgaggagg agtggtggag acgatctggt ttatcttcgt ggcgctgatg gtcaccatgt
     3361 acgtcctgtt ggacggcttc gaccttggcg ccggcgcaat acacctgttc gcggccagaa
     3421 acgaccggga gaggcgcacg atcatacgcg ccataggccc cgtgtgggac ggcaacgagg
     3481 tctggttgat cgcggccggc ggcacgttgt tcttcgcgtt cccgaccttg tacgcttccg
     3541 gcttctccgg gttctacctg cccctcataa tcgtgctctg gctactgatg atccggggcc
     3601 tctccatcga gctgcgcggg cacataagcg acccaatgtg ggcctctttc tgggacgctg
     3661 cgttcttcgt cggaagctct ctgctcgcaa tcttctacgg cgccgcccta ggcaacgtgg
     3721 tgcgcggcgt cccgctggac gaaagcggct acttcttcca gccgctgtgg acggacttca
     3781 gcccgttctc gggcaccccc ggcatcctcg actggtacac ggtcctgatc gggctgctcg
     3841 ccctgaccac tctggtcgtg cacggctcga acttcatcgc cgtcaagacg gaggacgacc
     3901 tgaacgcccg ctcgcgccgc atcgcgcgcc gtttctcctt cgcgaccatc ctgctcacct
     3961 tgttcgccac cccggccacc ttctgggtta gtcccgaggt gctctggagc ttcaacgaac
     4021 gcccctacgg gtacgtcctg ccgctcgtag ccgtggccgg gctagcaggg atggtctact
     4081 tcgacctcag gggccgggac agggaagcgt tcctctcctc cagcgcctac atcgtcggga
     4141 tgcttaccag caccgtcttc ggcgtctacc cgaaggtgct gcctgcggtg gacccggcga
     4201 acagcctcac ggtccagaac gcctccgcat cgacgtacgg catggcggtc ggcctcgtct
     4261 ggtggagcat cggaatggtc ctcgccgtgg tgtactttac cgtcatctac cgcctgttcc
     4321 gcggcaaggt gcgcctgtag tcgtctcctc gcataccgaa cagtccacga tgttgaccga
     4381 acaagcttta gcacggcaga gtacgtaaga aatggcccga aagatgcatg accgggcggc
     4441 atgtcggtgg caacatgaat gtcacaaaga gaacggagaa agagctgtct tatgcggaag
     4501 tgacggttca ccacatcgag gcgtaacgga cgaccatccg gcgccgggca aaacgttccc
     4561 tcgagtccga agggtgagac gacagaaggg tagagtagga cgtgttctac gaaacaacgc
     4621 acgcgtggaa gggcagttcg atgcgttctc cgtggtggct gatcgcgagg ggcccgacag
     4681 accacatgga ggtgttcacg ctcgatggcg gtagcatcct gcccgtcttc agcggcgagg
     4741 acgaagcgga gatgtacctc tggttcgagg aggcgtgcga tgatggctgg gaggtgcgca
     4801 ggagctcccc agaggagctg atgtcggtgc tctacggacc atgctcggac gccggggcag
     4861 tggccctcga tccctcgccg gagatcatcg acgggccgtc tgcctggctc gttagcctga
     4921 accggaggcg gttcttggga tggatggtct cgggaaggat tcccgtctca tgagggttca
     4981 gtacctgagc ctgacagtta agcgagaaag catgagtaga gcatgcccgg agactacgat
     5041 ctgatcatcg aggacgccga actttacgag aagctacgcg aggtggagcg agcgaggaag
     5101 agattgcggt gatcgccaac acaggccgct cggccgcagg taagcgaagc actccaggag
     5161 ctgtcctaga gcgagttgca agagcgtgcc aggggatcat cgtaggggga cgctccagga
     5221 cggacaagga tgggcccatc gatgccctcc gcaactactg aagggaccga gagccgtccg
     5281 gattctggcc atcatgcacc tttgtgtgca gtccactaca cacttcggcc caaaataccc
     5341 gtaccggcga ggttacgtgt ccggtttcac agcccaagga gcgggtggcc gcgcaaagta
     5401 acatcgatta tggaaggcag tgacgatacc atggcgcacc tgtcgggttc gtctatgagc
     5461 gcgcaaggcg gtgcaggcct tgctttgatc gcgttcatac tcgagcacgc cgggtgcgcg
     5521 ttggtcgagt ccaggttggg ggaggatctg ctggcctgct ggtgcccgca atgcaacgag
     5581 gcacagacct tcggtactct ggcatcgagg acgtatacgg cacccggata acaccgaata
     5641 gcgttgctta ctctatggac tgaacccacg gccccctcca gtagaaagcg accccttaca
     5701 caccgtgaac gtagggagag aggatgacca aggactacga gcagagccag acgatagacg
     5761 ccccgcccga ggaggttttt gcctggctcg cggacgtgga caacctcccg aggtatctgc
     5821 cacctgtcgt cagttcctcc gctgaggggc cgtctgcgga gggaaagcct ggccgcaggg
     5881 taaggacgac gctggagtat ccgggcggtg agggcacctt cgacgcccag ggttacctcg
     5941 ccttcgacga gctggagcgc aggatggagt ggggcgccga ggcaggacga gactactcgg
     6001 gatggctgaa ggtgggcagc cacggccagg gagggagcga ggttgtcgtg cacctctcct
     6061 tcggggagcg ctcggtggag ggtgagatcc agtcggagtc gccggaaggg caggatccct
     6121 tgaccgaggg catcctggcg accctcgagt cgatccgccg tcaaatagag gagggcgcgg
     6181 gcaaggtcca gcctcctcgg tccggcgtcg agccggacac cggggagaac ccggcggttg
     6241 tccgcgacga cccgccgtcc ggcgcccccg gatcctgaga agctacctcg atgatcgaac
     6301 acaggctcga aaccaggatt agaacatccc ccgaagccca tcgtgcagat ttatccccag
     6361 aacgggtgcc cgcgaagacc gagcccgccc gtggatctgg gtcttcgagt gttacgagaa
     6421 gaagcctgag gctcggagca caacccatgc ccgctgcgag atgaagggag tgatgatcac
     6481 acccagaaca gccggagttt gaacgtctat tccgaggtac ttcatactga aaaacgcagg
     6541 acgaagggag tcgcaaatgc cccagcgcgt acaagagagc gtggaagtcc aggccccact
     6601 ccaagaggtt ttcgactact ggtcgaactt cgagcacttc cccgagatca tgagcaacgt
     6661 cgaggaggtg cgtgtgatcg accgggagag aagccactgg aaggtcaagg gtcctgtcgg
     6721 taccagcgta gagttcgacg cgaggaccac ggagatggac ccggcccgcg ggataggctg
     6781 gaacagcgtc gagggtgagg tggacacctc gggcgaggtg cgcttcgagg aggtcgcttc
     6841 cgacaggaca cgcatcgagg tgacgatgaa ctacgccgac ccccctggcg gtaaagtggg
     6901 tgaggtcgtc gccgacgcga tatccaatcc ggagcgcatg atgcgcgagg acctcgaaaa
     6961 cttcgccgcg agagtagagc ggggcgaggt agagttcgcc gggccgggct cccaggcaca
     7021 gggtagataa gcagcgaatg tccatcatct gccgcgtagg ccgggtgaca tgaaggccgc
     7081 ggtgctcagt tgtacgttga aagcgcggcc catcccgccc gagcagcgag gttgatcggc
     7141 agtccagccc ggtgcaccgg gcaagaagct cagccgagag ttggcaggct gaatgaccaa
     7201 ttgattcaat cttcgatcct gagatgatcc ggcgccctgg gggcagggtc gggtagcacg
     7261 agtggtgagc gtccgggcgt aacgctgcca agcactaccg ccgagcgcgc ccctgtgcag
     7321 gagggcacgt tcgacggcag accgtgcacg ctcagaaatc ccatcagggc gaaggcatag
     7381 gcttctttgg cgcccgaggg gataccccag tcctcgatcc tgcgcagccg cacctccttg
     7441 gcgtcctccc gcagcatgcc catcagtgtg gggttcgacg ttcccccgcc cgacgcaacg
     7501 acctcgagaa tgtcgtagcg ctccagcgcg tccgcaaccg tccgcgaggt cagcgccgtg
     7561 acggtcgcta ccaaatcgtc agcctcgatg tccccgaacg gcgcaagacg ctccaggagg
     7621 tagggcaggt tgaagagctc ctttcctgtg gacttgggtg gccccagccc gtagtagggt
     7681 tccgcgagta gcgaacggag gaggccctcg tggatcccac cccgtgcggc cctcctgccc
     7741 tcgtggtcgt aggtctctga gccgtcggtc aggtactcta cggccgcgtc cagaagggcg
     7801 ttggccggcc ccacgtcgaa ggcgaatgga gtgtccccgg cttcgccgtc gggcacgacc
     7861 gtgatgttgg cgatgccgcc gaggttgagc gcggcccgcc tcctttcgcc gctcccgagc
     7921 agcaagacgt cgaagatgct cgccagaggc gccccttgtc cgcccgccgc tatgtcacgg
     7981 ctacgcaggt ccgacaccac gggcgagccg gtccgatctg cgatccaggc cggctgcccg
     8041 agctgcagcg tgcccagagc cttctcgccc tccacccagt ggaagacagt ctgcccgtgc
     8101 gagacgatga gatccgcgtg cccgccacaa aacgccagcg tggcatcggc cgcggcctcc
     8161 gcgaacgcac gccccaggcg cgtgtcgagc atgcaaaccc cacccgcggt cgtggcggcg
     8221 gggggcaacg cttcggcgac cgccgtccgc agccctgtat cgtaaggtac gccgcgcgac
     8281 cccaggggtc gcagcaccac agcgtcgcct tcgaggcgta gatcagcggc tgcggtctct
     8341 atggcgtcga aggaagtgcc tgagattatt ccgactacct tcaccgctgc ctccctgtca
     8401 cgtccctggc gaggtcgccc ctggccccgg atctcccgcg caccgcccgg cccgtgttcc
     8461 caacactacc atcgaaacgc tctggcgcgc gccggctgcc acaagatatt agtatgttgc
     8521 gtgtaccaat gtacgcgaag gccgggagaa ggtggcgctg caagcgacgt aggtgacccg
     8581 agcgcatggt ctctcgtcgt tgccgataaa ggaggagagc atcatggctt ctgtttcgga
     8641 aggacgaggt ggatcgccgc tccggagcac gggggaggcg ctaagggccc tctcggccgg
     8701 ttgcgtggga aatttcgtcg agtggtacga cttcgccatc tactcatact cggtccccat
     8761 aatcgcaacg gtcttcttcc ccgagggcaa ccgtaccgca gccatactct ctgctttcgc
     8821 cctctacggc gtcgcgttcc tggcacgtcc tttgggcggt gtattctggg gcaacctggg
     8881 ggacagggtg ggccgccgca acacgctcgc cgcgatcgtc ctcctcatgg ggggaggaac
     8941 gatgctgata ggcctcctgc ctacctacgc gcaggtcggc ctcctcgccc cgaccttgct
     9001 cgccctcctg cggctcgtcc agggtttctc gggcggcggg gagttcaccg gctcgacctc
     9061 cttcataacg gagtacgccc ccgaagggag cagggggctc tacgccgcca tctcggcgac
     9121 tttcacgacc ctgccttcgc tgctcggcgg gctgaccgtg ctggccttca ctgcggtaat
     9181 gtccgacgcc gcctatgctt cgtggggctg gcgcatcccg ttcttggtgg gaggtccgct
     9241 ggccctcgta gggctgtgga taaggctcag gatggaggag acgcccgcgt tcagaagggt
     9301 ccagagcacc gagcacgtcg agggcgtccc cgtcagggag gcgatccggc accacagtag
     9361 agagatagtg ctcgtcttcg cgatcgcttc ccttagcgcg ctcggggcct acaccttggg
     9421 ggcttacttc gtcacgtacc tgcaggaggt agtcggactc gacccgacga ccgcgatact
     9481 cgccaacttc acggctttct tcgtgaccgt gccgctggtc ccgctcgtcg gggccctggg
     9541 agaccggatc gggagaaaac ccctgctgtt catcggttcc gtgggcttcg tcgtcttgag
     9601 cgtgccgggc tatttgctgg ccggcagcgg gggcctcgtt accgccatac tgggccagct
     9661 tctggtggcg ctgccgtgga gtttcgtcgt ctcggccgtg gtcgtcacgc aggtcgagat
     9721 cttcccgacg agggtgcgct acagcggcgc ctcggtcggc tacaacgtcg cctacatgat
     9781 cttcggcggg accgcgcccc tggtggcgac cttcctggtg gcgcggacgg gcagcgacat
     9841 cgcccccgga gtctacctcg tggctgtagc cctggtcgtg ttcttcgtcg ccttcgcggt
     9901 gccggagacc tacaggttct ccctgctcag agagggcgaa gagcgacgag agggttctct
     9961 ggacgcgacc ggcgcacggc cctcataagg ggacgaaagc cgccgaggag ggcacgcagt
    10021 tggcgaaaag gccagtgatc accgagcagc gtaaccaggc cagcgcgggg ctggacgaga
    10081 tgcctgtgga gtcgatcctc tacctcatga accaggagga ccggcgggtc cccgaggcag
    10141 tagcggaggc cctgccacag atcgccgagg cggtcgggtt gatggtcgag gcgtggcgtt
    10201 cggggggacg ttggatctac atcggagcgg ggacgagcgg ccgcatcgcg gccctggatg
    10261 cggccgagtg ccaacccacc ttcggggtgc cacaaggccg ggtagtggcg ctgatcgcgg
    10321 gcggagaggc cgccctcacg cggtccgtag agggcgccga ggacgaccgc gcagcggccg
    10381 tcaaggacct cgaggactcg agcctggagc cgggcgacgt cgtggtaggg atcgcggcga
    10441 gtggacgtac cccttacgtc gtggcaggcc tcaggtacgc ttccggaatg ggatgcccca
    10501 caatcggtat cagcaacaac ccaggtagcg agctcggcgc cgcggccgac gtctgcatcg
    10561 agttgatcac aggggcggaa gtcctgaccg gttcgacccg gctcaaagcg ggaacctcgg
    10621 agaagctggt cctcaatatg ctctccaccg cagctttcgt tcgcctggac aaagtgtacg
    10681 agaacttcat ggtggacctc caggccggta acgagaagct gaaggagcgc tcccgacgca
    10741 tcgtttggga agccgcaggg gttacaagag acgaggccgg ggaactgctc cgcgccgccg
    10801 gcggctccgt caaggtggcc gtggtgatgg gcaaggctgg ggtcccggcg gacgcggcca
    10861 gggagctgct cgctgcggcc ggggggaacg tgcgccgggc gttggacgca gcgaccccgc
    10921 cggatcgaga atggtgaccg agagccggta agcgatgagc cgctacatcc taatcgatgg
    10981 gggacagggc ggctgccgca tcgtctacct ggcggacggg aaacagatac ttacgggcag
    11041 cggtgccggg ctcggccgtc gggctcggga ccgctccgca ggcgtacttg gtgtgctcga
    11101 acgggccttc gcggatctgg ggccgcgcgc ccccgatgcc gtcgacgcga tcgtggccgg
    11161 tcttacgggg ttcgacggtg cgtccgagac ggccggtgcg gtcgctgacg gcgtccgaaa
    11221 gctggttcgg agtggacgcg tagtcgtgac caacgatgcc gtgacctcct acctgggggc
    11281 cataggcttc gagccggggg cggtggtcgc ggccggtacc ggggtcatag ccctcgccgg
    11341 ggatagagac gggaacttcg cccgcagcga cggctg
//
LOCUS       DATMVF010000078         5452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_104674, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000078 DATMVF010000000
VERSION     DATMVF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104674"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VN178_03390"
     CDS             <1..257
                     /locus_tag="VN178_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWS80043.1"
                     /translation="GRVYVHLDLDVLDPEKAGKANEFAPAGGLSAEDLKMALGLIRER
                     FAVVAAGIASYDPTFDPDGRVLAVALACARILTAPAKSAV"
     gene            786..944
                     /locus_tag="VN178_03395"
     CDS             786..944
                     /locus_tag="VN178_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80044.1"
                     /translation="MELGIVSHGTTSKKLDEQLSDAEISQLVARKLVAQCSILVVIAG
                     IVSWLIAT"
     gene            1745..5275
                     /locus_tag="VN178_03400"
     CDS             1745..5275
                     /locus_tag="VN178_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HWS80045.1"
                     /translation="MGNKPLRILFAIAEAYPFIKVGGLADVGASLPKALARLGHDVRL
                     VLPGYSCVGAGRPTVSLETPMGSETELVRVNHHGVHGGVSVYALGNESYFDREAVYGG
                     YEDDDVAPFVLFSKAVVAFAARTEWKPDVIHCNDWHLGLVPQYVRYGPDRAALGRTRT
                     VFTIHNLAYQGHLGPETEALAGLEDSEGSMLARGIAFADAVNTVSRSYLEEILTVEHG
                     MGMDGLLRSRMDDLYGILNGVDYEEFAPETDPYIAACYDGSFVEGKRKNKTVLQKLSG
                     LEPDPDAPLLGMVARLVDQKGVDLLCGSLDEMTALDAQVVIMGRGDEHYERSLEDAVS
                     RHPGVVAYHATSEEALARQVYAGSDFFLAPSTFEPCGLGPLIALRYGSIPIARRTGGL
                     AETIRDHARYPESGLGFTFGRRSPGSLVKAVSRALKTYSRKSEWQSLQGRAMGANFSW
                     EDAGRAYERIYARTLAGRRTTDFSRNKGAPLTHGGFRGRARTKRAVPLFLVHHANQYL
                     ITDGYDNREGITEIVEGYAAALRLHGKYGIPASLHLSGTLIETLAWHYPWFLELVRDL
                     REKGLISLIGGTYSENVMPLFEPSFNSRQLDEFLWLYHRHLGRPPEEVEVCWVPERVW
                     DTEKLAPTLTSSELANGGYRYVLLDDRLLYPTNGSYAGSQRARFDSIGPFGSSAGGSH
                     SYDEPALAETHRAYRIAGASGLMMVPISANLRYWVPPSFPEHWRSLEKTVESLSQNGA
                     DDTLLVYADDLEITAGVGGWDASALGRYDAFLRWVASREDVAPVSMPEWLAEHPPQGE
                     RRLEPGTFFEVAQQWEAGEDYRGWWDSVAWAPYKEKLVRAQRALRQAQREGVDRRLLD
                     LAWKHLLASAYETAWYDPVEDGSVAAPWAKAVASHARACLVMVDAARWFARTERPPSA
                     EVADIDEDGEDEVILKGECLYAVLAPEDGGRLVYLFTLTPEGGALVVGNPTDDWNFQE
                     ELNRYMDHPPNHPGALADAGFEHDSYLVSALSDGTHAWVEMTNVEEGSRLFGARKSVL
                     LAPGNPALAVCYRMPEGSGDLATEACLGPDYYRLLREGRRGLSPCTGESRRGFRNGDV
                     AVWLGLAGDEQTGWTEPARPEAGHGMNVRVRAEASHFHLLISCGDTDEDSCQQLLQEH
                     RAALHEIGKRTGAVHLRGGVLLCGNGESKC"
     gene            5269..>5452
                     /locus_tag="VN178_03405"
     CDS             5269..>5452
                     /locus_tag="VN178_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80046.1"
                     /translation="MLRGIFRRFASSGGQGERSALVTLQAFLPFVRPHWRGFVPALIG
                     VIAVSLVALLKPWPLGF"
BASE COUNT         1034 a         1668 c         1765 g          985 t
ORIGIN      
        1 tcggcagggt ctatgtgcat ctagatctcg acgtcctcga cccagagaaa gccggcaagg
       61 ccaacgagtt cgcccctgca ggcggcctga gcgccgaaga tctaaagatg gccctcggtt
      121 tgatccgaga gcgcttcgcc gtcgttgcgg ccggcatcgc ctcctacgat cccaccttcg
      181 accccgacgg gcgcgtccta gccgtcgcgc tcgcctgcgc gcggatactc accgccccgg
      241 cgaagtccgc cgtatagaga tcggtcaagg ctcctactct gtcccgccga agagattccc
      301 taggagctct cggccttggg acgtgttcag ggagcgggca ggcttccacg cagaccgaac
      361 cttgcgatgc gacggagtga ggagggcgta aataagtgcg tcggctcatc tactcctctg
      421 aatctcagcc gattcttagc cgattctcaa tactttctca tactgcgcag ttaaatattc
      481 tggtcatgag agctcgaccg gataggaagg ggaacataca gggccggtac agagtgaact
      541 agatgagcat gcccgggagc ggctagcaca ccactttact ctccgatccc acctactcac
      601 aggagaagta tatgaggtca gtggaacaag tccaactcaa ggagataccg gggcgaccac
      661 accgtctcgg gagttatcca aaccgaggta tgcgcaatcg atttgttcgg gattggggag
      721 gcgaccccgc aggccagcca accgcgcgtg gcgtacgcca ggccggaaga gcggggagct
      781 ggatcgtgga actcggaatc gtctcacacg ggacgaccag caagaagctg gacgagcagt
      841 tgtccgatgc ggagatctca cagctggtgg ctagaaagct agtggcccaa tgctcgattc
      901 tcgtcgtgat cgccgggatc gtctcgtggc tcatagcaac atagggatag gcttacgcca
      961 agcctctttc catgatatgc tgcggacccc agagtaggtc ccgcctttcc tctcaccctt
     1021 gttccccaaa tgacctggaa ctagaggtcc ccgagataca gccgccgagt aaggcgagag
     1081 tgcccggcaa ttcccgttgt gccacaacgt agccttcccc gatgtgttag ctttcgagat
     1141 ccctccaaca ccattacaag cttcctcggc tagtatgcgt cttaactctg aaaacttgct
     1201 gagaaagtag ccacgacctt tccctgatgg cgctccagta aggatagttc accgaggtgg
     1261 ccttctacga actttgcctg tgagtgaatt cgtgcaagct tcacgtgcag gagggttcag
     1321 atcattcgcg cttagtcaga agaggtgcca tctgacagat acaactcagg ggctagtttg
     1381 acaactctgg aatctttgag aaaattctac aaaatatgaa ggttgaggaa actctatacg
     1441 tatatggagt ttacaggtac aatttaagga ctagtatgag acaattgtaa gattagcttg
     1501 aaagtgcgcg caggagaacg cctcgggggg agaagcgtac gattgagggt gtagagaatg
     1561 ctcgaacatg gggtccaggc cggtccgttt cgaagccgcg ggtcggcgct cagtggcttt
     1621 ttggccagct agctgcgccc ggagctgcct gcggggcttt gctgtcagct atgcgatccg
     1681 cgatgctagc gcgcctgtgc ttcccgcctt gcgaacccat ggatgaagga ggtagtggtt
     1741 agcgatgggc aataaaccct tgagaatcct attcgccatc gccgaagcct atcctttcat
     1801 caaggtcggt ggactcgccg acgtgggagc ttctctgccg aaggcgctgg cccggctcgg
     1861 ccacgacgtg aggctggtgc tgccggggta ctcgtgcgtg ggggcgggga gaccaaccgt
     1921 atcgttggag accccgatgg ggtcggagac ggaattggtc cgcgttaatc atcacggcgt
     1981 gcacgggggc gtaagcgtct acgcgttggg caacgagagc tacttcgaca gggaggcggt
     2041 gtacggcggc tacgaggatg acgacgtcgc ccccttcgtg ttgttctcca aggccgtagt
     2101 ggcgtttgcc gctcgcacgg agtggaagcc cgatgtcatt cactgcaacg attggcactt
     2161 ggggctggtc ccgcagtacg ttcgttacgg cccggaccgg gcggcgttgg gtcggacgcg
     2221 gacggtgttc accatccaca acctcgccta tcaggggcac ctgggaccgg agacggaggc
     2281 gctcgccggg ctggaagaca gcgagggcag catgctggcg cgggggatag ccttcgccga
     2341 tgccgtgaac accgtcagcc gaagctacct ggaggagatc ctcaccgtcg aacacgggat
     2401 gggcatggac ggcttgttgc gctccaggat ggacgacctt tacggcatcc tcaacggtgt
     2461 tgactacgag gagttcgccc ccgagacaga cccctacatc gccgcgtgtt acgacgggtc
     2521 gttcgtcgaa ggcaagcgga agaacaagac cgttctccaa aagttgagcg gtctggagcc
     2581 cgatccggac gcgcccctgc tcggcatggt ggcccgcctc gtggatcaga aaggcgtgga
     2641 ccttctgtgt ggcagcctgg acgagatgac cgccctggac gctcaggtcg tgataatggg
     2701 ccggggagat gagcattacg agcggtcgct ggaggatgcg gtgtcccgtc accccggcgt
     2761 cgtcgcctac cacgccacca gcgaggaagc gctggcgagg caggtatacg ccgggtcgga
     2821 cttcttcctg gcaccctcta ctttcgagcc ttgcggcctt gggcccctga tcgcgctgcg
     2881 ctacggctcc atccccatcg cccggcgcac cggcggcctg gccgagacta tcagggatca
     2941 cgcccggtac ccggaatccg gcctgggctt cacattcggc cgccgctctc cggggtccct
     3001 cgtgaaagcg gtgagcagag ccctcaagac atatagccgc aagagcgagt ggcagtccct
     3061 gcaagggagg gccatgggcg ccaacttctc gtgggaggac gctggcaggg cgtacgagcg
     3121 gatctacgcg cggaccctcg ccggccgccg gacgactgac ttctccagaa acaaaggcgc
     3181 gccactcacc cacgggggat tccgggggag ggcgcgcacc aaacgggccg tgcccctgtt
     3241 cctggtgcac cacgccaacc agtacctcat caccgacggc tacgacaacc gcgaggggat
     3301 cacggagata gtggagggct acgcggcggc gctcaggcta cacgggaagt acggtatccc
     3361 cgcgagcctc catctgagcg gaaccctgat cgagacgctc gcctggcact acccgtggtt
     3421 cctggagctg gtgagggact tgcgggagaa gggactcatc tccctgatag gcggaacgta
     3481 ctccgagaac gtgatgcctc tgttcgagcc ttctttcaac tcgcggcagc tcgacgagtt
     3541 cctttggctc tatcaccgcc acctgggccg cccgccggaa gaggtcgagg tctgctgggt
     3601 gccggaacgc gtctgggaca ccgagaagct ggcgcccacc ttgaccagca gcgaactggc
     3661 aaacggtggc taccgctatg tcctgctgga cgaccggctg ctctatccca ccaatggttc
     3721 ctatgcgggc agccagcgcg cacgattcga ctcgatcgga ccgtttggct cgagcgccgg
     3781 cgggtcccat tcgtatgatg agcccgcttt ggctgaaacc cacagggcct accgcatcgc
     3841 cggggcgagc ggcctgatga tggtgcccat ctcggccaac ctgcgctact gggtaccgcc
     3901 cagcttcccc gagcattggc ggagcctgga gaagacggtc gaatcactca gccagaatgg
     3961 cgcggacgat accctgctcg tctacgccga cgacctggag ataacagcgg gcgtcggcgg
     4021 ttgggacgcc agcgccctcg gacgctacga cgcctttctg cgctgggtag cgtcccgaga
     4081 ggacgtggct cccgtctcga tgcccgagtg gttggccgaa cacccgccgc aaggagagcg
     4141 caggctcgag cccggtacct tcttcgaggt ggcgcagcaa tgggaggcgg gcgaagacta
     4201 ccgcggatgg tgggatagcg tcgcgtgggc gccctacaaa gagaaattgg tccgggccca
     4261 gagagcgctg cgccaggccc aaagggaggg tgtggaccgg cggctgctgg atctcgcctg
     4321 gaaacacctc ctcgcctccg cctacgagac cgcctggtac gaccccgtcg aggacggctc
     4381 cgtcgccgct ccctgggcca aggcggtggc cagccacgcc cgcgcctgcc tggtaatggt
     4441 agacgccgcc cggtggttcg ccagaacgga gcggcctccc tccgcggagg tcgcggacat
     4501 agacgaagac ggcgaggacg aggtgatcct gaagggcgag tgcctctacg ccgtcttggc
     4561 gcccgaggac ggcgggaggc tcgtctacct cttcaccctg acgccggagg gcggcgccct
     4621 ggtcgtcggc aatcccaccg acgactggaa cttccaggag gagctcaacc ggtacatgga
     4681 ccatccgccc aaccatcccg gcgccctggc cgatgcgggt ttcgagcacg acagctacct
     4741 tgtttccgcc ctcagcgacg gcactcacgc ctgggtggag atgaccaacg tcgaggaggg
     4801 cagccgcctg ttcggggccc gcaagagcgt gctcctggcg cccggcaacc ccgccctcgc
     4861 ggtctgctac cggatgcccg aggggtccgg cgacctcgcc acggaggcgt gcctcggacc
     4921 tgactactac cgcctgttac gcgaagggcg ccgtggtttg agcccgtgta ccggcgagag
     4981 ccggcgcgga tttcggaacg gagatgtcgc cgtctggttg gggctggccg gcgacgaaca
     5041 gaccggctgg acggaaccgg ccaggccgga ggcaggacac gggatgaacg tccgcgtgcg
     5101 ggcagaggcc tctcattttc acctcctgat ctcctgcggc gacacggacg aagactcgtg
     5161 ccagcagctc ctccaggagc acagggcagc actccacgag ataggcaaga gaacaggcgc
     5221 cgtgcatctc agaggcggtg tgctgttgtg tggtaacggg gagagtaagt gctgagaggt
     5281 atcttccgac ggttcgcctc ttcgggaggt cagggcgagc ggtccgcgct cgtgaccctg
     5341 caagcctttt tgcctttcgt gcggccccac tggcgcgggt tcgtcccggc gctgatcggg
     5401 gtcatcgcgg tcagcctcgt tgccctgcta aagccctggc cattggggtt cc
//
LOCUS       DATMVF010000079        15303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_237235, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000079 DATMVF010000000
VERSION     DATMVF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15303
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237235"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VN178_03410"
     CDS             <1..182
                     /locus_tag="VN178_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HWS80047.1"
                     /translation="APDVADRALEALRKVEGGEEAAIIGEIREEPARMVLMHTRFGGA
                     RMIDMLVGDPLPRIC"
     gene            312..554
                     /locus_tag="VN178_03415"
     CDS             312..554
                     /locus_tag="VN178_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWS80048.1"
                     /translation="MSSITASEARANLYRLIDEAAASHQPLLISGKRNNAVLVSEEDW
                     TAIQETLFLLSVPGMRESIREGMDTPVDECGEDPGW"
     gene            551..814
                     /locus_tag="VN178_03420"
     CDS             551..814
                     /locus_tag="VN178_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HWS80049.1"
                     /translation="MSWRLVYTKQAQKDAKKLASSGLRPKAQELLDILAEDPFQKPPS
                     FEKLVGDLSGAYSRRINIQHRSVYQVLEEARVVKVLRLWSHYE"
     gene            1041..1544
                     /locus_tag="VN178_03425"
     CDS             1041..1544
                     /locus_tag="VN178_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80050.1"
                     /translation="MLNGTIADEQEILIACLAHLHLGERQVVRSLTRSGLQVPADERM
                     LSFLRENLGGLDEETLTDFLAKNRVQHPIEPDLNPGGRLICVGDEEFEHIFRDGDGWA
                     RFRKEFPDSDGTLRLSRVGFDRDVTRAMLYAGQQFDWNVGSGGFWLFSRLDGEWTEAG
                     RAGSWLS"
     gene            1938..2267
                     /locus_tag="VN178_03430"
     CDS             1938..2267
                     /locus_tag="VN178_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80051.1"
                     /translation="MGELILFLPLSLEMQTIVYLASRYNPGYTSGMKTAISIPEELFE
                     SAEEFARWRGMSRSELYTTALRRYLEEQRGEMITGRLDEIYGQEPDGLDRDIARLQVR
                     SLPEDDW"
     gene            2267..2605
                     /locus_tag="VN178_03435"
     CDS             2267..2605
                     /locus_tag="VN178_03435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWS80052.1"
                     /translation="MRRGEVWWAELPEPVASEPGYRRPVLIVQSDDFNRSRIRTVIAV
                     VLTTNLRLAGAPGNVPVSKEDTALSRDSVANVSQIITLDKAFLTERVSRVSDRIMMSV
                     EEGLRTVLAL"
     gene            2673..3854
                     /locus_tag="VN178_03440"
     CDS             2673..3854
                     /locus_tag="VN178_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWS80053.1"
                     /translation="MSRVDADALTDQSREELLRRNGVFEGFFEVEAPRLAQACHEMSR
                     RFLAGGRLLAFGNGSAATDAQHVSVEFVHPVIVGKRALPALDLGPDFEHRLPVVLRPE
                     DMVMGFAFPETDRPVERAIQWARERGALTFGLTGEAGEYSFTPPDGDPFVVQEVFEVL
                     YHVLWETVHVYFEHREQGHDVGNSSFLYPFLGKGQQPLEEVVREVQGSMLQKMQEVNG
                     LRSAAAESEARTISQIAVAVAERLERGGKLITFGNGGSATDANDLVSDCVDPPPGWGT
                     VPAVSLSAEPANITAIANDIGTEAVFARQLIAHARPEDVAVGISTSGGSANILAALAE
                     ARKRGLLTVGIVGYDGGRIVAERLADHAVVVRSDYIPRIQEAQASIYHVLRGAIERCM
                     G"
     gene            3883..4068
                     /locus_tag="VN178_03445"
     CDS             3883..4068
                     /locus_tag="VN178_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80054.1"
                     /translation="MRYVLLILGGLMILMGGVWLLQGIGILPGSFMTGQMFWAVMGAV
                     LLAVGGFLVIVGFRLGG"
     gene            4181..5506
                     /gene="hemL"
                     /locus_tag="VN178_03450"
     CDS             4181..5506
                     /gene="hemL"
                     /locus_tag="VN178_03450"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWS80055.1"
                     /translation="MQGSGESVDARTMLAMDRSARLMERSVGLMPGGVNSPVRAFRPV
                     GGDPLFMERGEGSRIFDVDGNSYLDYVMSYGPLVLGHAYPEVIEAVETTARKGTTFGA
                     PTELEVELADLVCGAVPSVEMVRMTNSGTEATMSAIRLARGYTGREKILKFDGNYHGH
                     GDALLVSAGSGVATLGLPDSPGVTKGAAKDTAVLPYNDLEAVRDLFAREGEAFAAIIL
                     EPVAGNMGCVPPKDGYLEGLRKITNDYGTLLIFDEVMTGFRLARGGAQQRFGVTPDLT
                     CLGKIIGGGLPVGAYGGSREIIDQIAPSGPVYQAGTLSGNPLAMAAGLATLRVAGQPG
                     FYDRLEDLGSRWREGMGEAAAAGSLPFTINQVGSMVSIFFTGGPVTDFGSAAASDTGT
                     FKGFFWHMLSRGVYLAPSQYEAGFISTAHSEADLQKTFEAAHEWFDGRE"
     gene            5507..5926
                     /locus_tag="VN178_03455"
     CDS             5507..5926
                     /locus_tag="VN178_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80056.1"
                     /translation="MQEPAGRTPDLSLEDTFLRAVAGLPEEDLRAGVVAEALGYLQQG
                     FDAHYAGDGATDDDVLVGDNAYALAVETIARLDEPRFVAVASRMIRDGAGRIAAGGAV
                     SLQTWTPHLAGLLDIISEEGEERSEARIRDAVEELRG"
     gene            5919..6650
                     /locus_tag="VN178_03460"
     CDS             5919..6650
                     /locus_tag="VN178_03460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HWS80057.1"
                     /translation="MGKGTRNRLPSGEQRAVLVREMFDRIAGRYDLMNTLMTAGLNRL
                     WNRKVLRATGLKSGDRALDLACGTGALTRDLAKKVGPDGYVLGVDFSREMLRAARPAQ
                     GVEYRFGDATDLQGVPSGAFDAATIAYGARNIPDLDALFSEMARTLRPGGVAVCLEIA
                     RPTGRVSSAFYGVWFDKIVPRLGGWISGDPSAYSYLPESVKEFVAPGELSDIIERNGL
                     QNVTWERVAGGIITLHRGVRPHDAG"
     gene            6565..7614
                     /locus_tag="VN178_03465"
     CDS             6565..7614
                     /locus_tag="VN178_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWS80058.1"
                     /translation="MDYKTLHGSASQAASSRSIVGSGHTTQASRLHTALPEGAFEIID
                     EVEGLLVGVAGNAPPDLVESSLEALTSGGKRLRPLLLVLSARMGEPRLEDLLGAATAI
                     EVLHTATLIHDDIVDRAESRRGVPTTAAGYGREIAAATGDYLFAEAFSELARIRDPRL
                     VRAFAEASVGLAAGELEQFRANGATVDVESYLEHIRKKTAGLFQAACVAGGTLGGLSL
                     VQIDALASYGQALGIAFQMSDDIMDLVGKPGLMGKGIGTDLVEGTVTLPVIFALSEGD
                     AGTIRRVLADPNPTPELLEAGIEAVLATDAVARTEEWARGEIEAALADIGLLPVGPER
                     EFLESVASEVVGRNV"
     gene            7607..8854
                     /locus_tag="VN178_03470"
     CDS             7607..8854
                     /locus_tag="VN178_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWS80059.1"
                     /translation="MSESRAEPGIFAAGTLMPISSPPLQDGAVLVREGRIGAVGDLAT
                     IGHENPGTEIRFFPNCTIIPGAVNVHAHLGFRRKDAPEDGAFSEWLRTLIERLPEKEA
                     WTAEAAGDSAREAVAAGTTYMAESSPYGECLPQLAESGMAGTVYAEFFPHEIGAPKEA
                     IDYIVAKVSDLRVGLPERVDAQVSVHSPYTVDPESSRLAARRTREMGWKLAIHLSESP
                     EEVEFLKSGTGGLADIFGSNEWGGIGVSPVRYAQGVELLAPQTIAAHLATGVSDEDIE
                     VLAQTGVAAAHCPRSNAYLRCGVSPVPAMIASGIRVGMGTDGLWSSPSMNLFEETLFA
                     VELHGMSGAAGLELATLGGARALGIEAGTGTLEAGKWADFAVVETLGEGRDPELGVLE
                     AAAGGGVAATVVGGDMIYNRLGT"
     gene            8877..9515
                     /locus_tag="VN178_03475"
     CDS             8877..9515
                     /locus_tag="VN178_03475"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS80060.1"
                     /translation="MMMNRDKLSFWVRLVSIVLAAFFLLSFIFLGLGTNISYNLFELI
                     GNQDQQQQQADQAPDPQEQIADAEKALEKDPKDPEKIKDLAALYYTAGRYDDAIQVLQ
                     RGREVAPQDEEIPTLIGQIYSQQAQAEKGEKQKELLKKAGDAFAASAAAEPDNAEVYL
                     LAAQAYDQAGQPADAIEYYNGYLDLEPKGENAKQVKDRISALLEGGGGSAGP"
     gene            9582..9857
                     /locus_tag="VN178_03480"
     CDS             9582..9857
                     /locus_tag="VN178_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWS80061.1"
                     /translation="MFGSVGMPELIIGLVVILLLFGAKRVPELARGLGSGVREFKKGT
                     QEGEIEDEKKEEAKKDEEQLEASKSDDETKLEEDASVEAEKAEQKHS"
     gene            9932..10762
                     /gene="tatC"
                     /locus_tag="VN178_03485"
     CDS             9932..10762
                     /gene="tatC"
                     /locus_tag="VN178_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWS80062.1"
                     /translation="MTLIEHLDELRSRIIKVGVAFFGVAIVAWFFRQRIFEWLLAPAG
                     GALQGKLNVTGVTEQLFTDMKLALYVGFLLTIPVLLYQVWAFVAPAVGDMGRVFTYTI
                     ISMASALFIAGVAFGYFIVLPVGTQFLLNWDPERFDPIITAQSYLPFVTRFLLAFGIV
                     FELPAATYVAAKLELVDAPLLKRYRKHAIILNTVLAAALTPGQDPYSMVLMAVPMIVM
                     YEISILIARYVNPTTQVTAHELVRDDGDESEEPEPDEPDIDDFEDTEDHPENGHERDL
                     "
     gene            complement(10759..11235)
                     /locus_tag="VN178_03490"
     CDS             complement(10759..11235)
                     /locus_tag="VN178_03490"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWS80063.1"
                     /translation="MPERHRPFKASYVLSGVPAERIFDSLLDVQRFPEWTAGLRRCRV
                     LDPAGKVETKVLRPGVRLEFTLSAAGLTHEVSSIVTVVEPPRRLEWNYVEGATGSGGW
                     LVEQEAPGAIRITLSTDYLIRPAWLDKLAHKPFFRRLTEDLLRRSMRRFDAHLRYG"
     gene            complement(11228..12130)
                     /gene="menA"
                     /locus_tag="VN178_03495"
     CDS             complement(11228..12130)
                     /gene="menA"
                     /locus_tag="VN178_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HWS80064.1"
                     /translation="MATSTRRQAFEEWFRLARPFSLTAAAVPVLIGTALAFKDGTFAW
                     GPFFAMLFGSLLIQAATNMFNEFYDAQRGLDVAGTVGIAGSIVSGRVSSRSVLAGALL
                     CYTLALFFGIYLIAVGGWPILVLGCLSALGGHLYSAGPRPIAYTPASEAAVFVFMGVL
                     IVVIAYAVQARGFPVYVPLAALPIGGPVAAILLANNIRDLVSDRRGGRRTLPVVFGRE
                     VAILVYRALLLEAYVAVAVLMAFGVVPPECALVFPSVFAALRLWRDVASYSTPSRLDP
                     VVKRTAGLHLVFGLLYTAGVLIGA"
     gene            12386..13309
                     /locus_tag="VN178_03500"
     CDS             12386..13309
                     /locus_tag="VN178_03500"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HWS80065.1"
                     /translation="MIQPAVVEAGSSSGLASSYLRLVRPRQWTKNGFVLAGVVFAEKA
                     LSATALADALLAFAIFCALSGAVYAANDVFDVEEDRKHPLKRFRPVASGEISARAALI
                     FAAVLAAGALAVSFALLPYGVGIAGIAYLVLQVFYTTILKHAAILDVMSISAGFVIRA
                     LAGVAAVSSPISPWLIVCTGLLTLFLGFSKRRHEIATLGEGAVVHRKNLQDYSVPMLD
                     EMMNIMISATIIAYTLYTFTVYERQDQIFMMGSIPFVVYGVFRYMLLVHRDGGGNPDT
                     LLLSDRPLQISLFLWLVVVGTAIYVIPLWNG"
     gene            13297..14256
                     /locus_tag="VN178_03505"
     CDS             13297..14256
                     /locus_tag="VN178_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWS80066.1"
                     /translation="MERLRRNLVLALALGAAVYLILAVLSDLGELVAALDSFDYALIP
                     AILGLVALSYVVRFFRWTYYLRVLKVSVPTGINAAIFTSGLSMTISPGKLGEVLKSVF
                     VRDATGTPIARTAPAVVAERATDGTGMVLWGFLGAFALTLPPWTMLMFLALAAFGIAV
                     LRSKRLSLAAESVLSKLPLLNRLAPHLSVFHGASNELLGTRPLLVGTAISFFSWGLEC
                     AGVYLCAVGLGVDRPFLLIVFVFATSSILGVLSMLPGGLGAVEAGLYGQFVAVANLST
                     GVAGALTLVIRLATLWFATLLGICGLLLVRRLLGDASIEPDKT"
     gene            complement(14275..14946)
                     /locus_tag="VN178_03510"
     CDS             complement(14275..14946)
                     /locus_tag="VN178_03510"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWS80067.1"
                     /translation="MRGFFGTGLGRFGIFTCAVIAMLAIGALALAGRGMIAGADAQPA
                     ERAIQPSGAEAPTPEPVAETEPENEASASPPATVPAGALMSLSVPAMGISDIPVVEGT
                     SEASLSQGAGHLPGTGYPWVAGSNTYIAGHRIGYPGTASDHVFWNLPNLGQGDRILLT
                     ASDGTTYTYAVSEILEVSPTDLSVTAPTGGDVVTLQTCIEDYGDYWTPGPNWYARYIV
                     RGTMV"
BASE COUNT         2922 a         4698 c         4937 g         2746 t
ORIGIN      
        1 tggcccccga tgtagccgac cgtgccctgg aggcgttgcg caaggtagaa ggtggcgaag
       61 aagccgcgat cattggcgag atccgcgagg agccggcgcg catggtcctc atgcacaccc
      121 gcttcggtgg cgcccgcatg attgacatgc tcgtcggtga tccgctgccc cggatctgtt
      181 aggtgtaata agactcaaaa tggatctcac ggctagagta aggacgtgtg gattgttaac
      241 cataaaacgt accatttgac gtacgtacgt ttttgcgtac aattttgcca aggatctagc
      301 gaaggtgtgt catgagttcg attacggcca gcgaagcccg cgccaacctg tatcgtctga
      361 tcgacgaggc ggcggcctcc catcagccgc tgcttatctc gggtaagaga aacaatgctg
      421 ttctggtatc cgaggaggat tggacggcca tccaggagac tcttttcctt ctgtccgtgc
      481 ccggcatgcg cgagtccatc cgagaaggga tggatacgcc cgttgacgag tgcggcgagg
      541 acccgggttg gtgagttggc gactcgtata tacgaaacag gcgcagaaag acgcaaagaa
      601 gctggcttcg agcgggctga gacccaaagc ccaggagcta ctggacatac tcgccgagga
      661 tcctttccaa aagccgccat ctttcgagaa gctcgtgggg gatctctcgg gcgcctattc
      721 gcggcggatc aacattcaac accggtcggt gtatcaggtg cttgaagagg cgcgggtcgt
      781 caaggtttta cggctgtgga gtcactacga atagggttat cggagacgat caaggtgagc
      841 acgggcttga aaccatgagg atgtccccgg atcaactcga tggatacgag agagctgggc
      901 cttcaagttc aaccccggtc gaaagctgcg caccttcttc ggctggtcct ccacttacta
      961 cgacccgctg tagtacggcc ccggcggcgt gaagcatcca acagccgcca aatcttgcga
     1021 acgtaccagg cgcatatcac atgctcaacg gaacaatcgc tgacgagcaa gagattctaa
     1081 tagcttgtct cgcccaccta cacctcggcg agaggcaggt cgtgcggtct ctgacccgca
     1141 gcggtctgca agtgccggca gatgagcgga tgctctcctt tttgcgtgag aacctcgggg
     1201 ggcttgacga agagacactg accgactttc tggcgaagaa ccgcgtgcaa catccgatcg
     1261 agccggatct aaaccctgga gggcgtttga tctgcgtggg cgacgaggag ttcgagcata
     1321 tcttccggga tggggatgga tgggcccgat ttcgcaagga gtttcccgat tcagacggca
     1381 cgctccgact ctctcgggtc gggttcgaca gggatgtgac gcgggcgatg ctctacgccg
     1441 gacagcagtt cgactggaac gtgggttccg gcggtttctg gctcttctcg agactcgacg
     1501 gagagtggac cgaagccgga agggcgggaa gctggctctc ataggctgca ttcgaccgat
     1561 ggacacagcc acactcgcgc atcggagcgt cactttcgac attacttctc cggcgcagcg
     1621 atatcttgga aggattcttc gaggctcagg caccgagcgt ggcacaggcg tgccacgaga
     1681 tttcccgacg ctttcttgct ggcgttcggt gtgttggaag aaggtcgtat gatcaatcga
     1741 gagtacggtc ttgtcgaagt gtcccccgcg agagggctga aggggcccac cagcggtggt
     1801 acgagcaagg acgcctgacc aggcgctgca ttacccgtgg aacaatcgcg aggagcattg
     1861 cgtcgtgagg ttcagacttc gccgctggtc gagccgcttg gctcgagctt gtagctaggc
     1921 ccggcgaacc ggaggagatg ggcgaactga tactcttctt gccgctgtcg ttggagatgc
     1981 agacaatcgt ctatctcgcc tcccggtata acccaggtta tacttcaggc atgaagacgg
     2041 cgatctctat tcccgaggag ctattcgagt cggccgagga gttcgcgcgg tggcgaggta
     2101 tgtcgcgcag cgagctctac acgacggcgc tgcggcgcta tctggaggag cagcggggtg
     2161 agatgatcac cgggcggctc gatgagatct atggccagga gccggatggt ctggaccggg
     2221 atatcgcccg cctgcaggtc aggtcgttgc cggaagatga ctggtagtgc ggcgcggaga
     2281 agtctggtgg gcggaactgc ccgagcccgt cgcctcggaa cctggatacc ggcgacctgt
     2341 actcatcgtc cagtcggacg acttcaaccg cagccgtatc cgtactgtga tcgcggtggt
     2401 gttgacgacg aacctgcggc tggcgggggc tcctggtaac gtgccggttt cgaaggagga
     2461 tacagcgtta tccagagact ccgtggcaaa cgtgtcgcag ataatcacgc tcgacaaggc
     2521 gtttcttact gagcgggtga gccgggtcag cgaccgtata atgatgtccg tagaggaagg
     2581 cctgcggacg gtgctcgccc tgtagaactc ggcgcttctc gtggagggaa ttgtttaccg
     2641 gaggccggat tgacgggctg gaggtacacg cgatgagccg ggtagatgcg gacgccctca
     2701 ctgatcagag tcgcgaggaa ctgctccggc gcaatggtgt cttcgaagga ttcttcgaag
     2761 tggaggcgcc taggctggca caggcctgcc acgagatgtc ccggcgcttc ctcgccggtg
     2821 gccggctcct ggctttcggg aatggctccg ccgcgacaga tgcgcagcac gtctcggtgg
     2881 aattcgtgca cccggtgatc gtcggcaagc gggcgcttcc ggcactcgac ctcggtcccg
     2941 acttcgagca ccgcctgccc gtcgtgctac ggcccgagga catggtgatg ggcttcgcgt
     3001 tccccgagac cgacaggccg gtcgagcggg ccatccagtg ggcacgggag cggggagcgc
     3061 taacgttcgg cctgacgggc gaggcgggag agtactcctt cacgccgccc gatggggacc
     3121 cgtttgtcgt ccaggaggtc ttcgaggtcc tctaccacgt gctctgggag acggttcacg
     3181 tgtacttcga gcaccgcgaa cagggccacg acgtgggcaa ctcctcgttc ctctatccct
     3241 tcctcggcaa aggacagcag ccgctcgaag aggtcgtgcg cgaggtacag ggttcgatgc
     3301 tccagaagat gcaggaagtg aacggcctgc ggtccgcggc tgccgaatcg gaggccagga
     3361 ccatctccca gatagccgtg gccgtcgccg aacggctgga gcgtggaggc aagctcatca
     3421 ccttcggtaa cggcggctcg gcaacagacg ctaacgacct cgtctcagac tgcgtagacc
     3481 cgccgcccgg ttggggcacg gttccggcag tctccctgtc cgccgagccg gccaacataa
     3541 cggccatagc caacgatatc ggcacggaag cggtcttcgc ccgccaacta atcgcccacg
     3601 cgagaccgga ggatgttgct gtcggtatct ctaccagcgg gggctctgcc aacatacttg
     3661 ccgcgctcgc cgaagcccga aagcgaggac ttctgaccgt cggcatcgtg ggatatgatg
     3721 gaggtaggat cgtcgccgag cggctggcgg atcacgcggt agtcgtccgc tcggactaca
     3781 tcccgcgcat ccaggaggca caggcctcta tctaccacgt actccgcggg gccatcgaac
     3841 ggtgcatggg ttgagccccg gatctggaat gggaggtatc gtatgcgcta cgtgctgctg
     3901 atcttgggag ggttgatgat cctcatgggc ggcgtctggc tgttacaagg catcggcata
     3961 ctgccaggta gcttcatgac cggacagatg ttctgggccg tgatgggcgc agttctgttg
     4021 gccgtgggag gatttctagt catcgtcggg tttcgattgg gcggctgaga gtagatgagt
     4081 acagcccgcc tattacccat tttggggtaa gctctgtcat ggcccggaca ggtccaggat
     4141 aatagacttc aaaccgacct agcgaaaggg gctggcgcgt ttgcagggta gcggagagag
     4201 cgtagacgcg aggacgatgc tcgccatgga ccgttcggcg aggctgatgg agcgttccgt
     4261 cggactgatg ccgggtggcg tgaacagccc cgtgcgggcg ttccgcccgg tcggtggaga
     4321 cccgctgttc atggagcgcg gtgagggatc tcgcatcttc gacgtggacg gcaactccta
     4381 cctcgactac gtgatgagct acggtcctct cgtcctcggt catgcttatc ccgaggtgat
     4441 cgaggccgta gagaccacgg ccaggaaggg tacgaccttc ggcgccccga cggagctcga
     4501 ggtcgagctt gctgacctcg tgtgcggggc cgtcccttcc gtggagatgg tcaggatgac
     4561 caactccgga acggaggcga cgatgagcgc catccggctc gcacgcggat acacgggaag
     4621 agagaagata ctcaagttcg acggaaacta ccacggccac ggcgacgccc tcctagtctc
     4681 ggccggatcc ggagtcgcga cgctaggcct ccccgacagt cccggcgtca cgaagggggc
     4741 cgcaaaagac accgccgtcc ttccttacaa cgacctcgag gcggtccggg atctcttcgc
     4801 gcgcgaggga gaagcgttcg ccgcgattat cctcgagccc gtcgcaggga acatgggctg
     4861 tgtcccgccc aaggacggct atctcgaagg tctgcgcaag atcacaaacg actacggaac
     4921 cctcctcatc ttcgatgagg taatgaccgg tttcaggctc gcccgtggtg gcgcccagca
     4981 acgtttcggc gtcacgcccg acctgacgtg cctcggcaag ataatcggcg gtggactccc
     5041 cgtcggcgca tacgggggga gcagggagat catagaccag atcgcaccct cgggccccgt
     5101 ctaccaggca ggcacgctct ctggcaatcc gctggcgatg gccgcgggac tcgctaccct
     5161 gcgcgtcgca gggcaaccgg gtttctacga ccggctggaa gacctcgggt cgcgctggcg
     5221 ggaaggcatg ggcgaagccg ccgctgccgg aagcctaccc ttcaccataa atcaggtcgg
     5281 ctcgatggta agcattttct tcaccggcgg ccccgtaacg gacttcggat cggcggccgc
     5341 gtcggacacc gggacgttca aggggttctt ctggcacatg ctctcccgcg gggtctacct
     5401 ggccccgagc cagtatgagg ccggctttat ctccacggcc cactctgagg cggaccttca
     5461 gaagaccttc gaggcggcac acgagtggtt cgacgggcgg gaataggtgc aggagccggc
     5521 cggccgcacg cccgatctct cgctcgagga tacgttcctc agggccgtcg cgggtcttcc
     5581 cgaagaggac ctgcgcgccg gggtagtggc cgaggcgctc gggtacctgc agcaaggctt
     5641 cgacgcccac tacgcggggg acggggcgac cgacgatgat gtcctcgtcg gggacaacgc
     5701 gtacgcgctg gccgtagaga cgatagcccg tctggacgag ccgcggttcg tcgccgtggc
     5761 gagccgtatg atccgcgacg gcgcaggacg catcgccgcc ggaggagcgg tctccctaca
     5821 gacgtggact ccccacctcg caggcctcct cgacataatc tccgaggagg gagaagagag
     5881 atccgaggcg aggatcaggg atgctgtaga ggaactgcgt gggtaaggga acccgaaaca
     5941 ggctgccctc gggtgaacag cgggcggtgc tggtgcgcga gatgttcgac cggatcgcag
     6001 gccgctacga cctcatgaac accctgatga ccgcgggcct caaccgtctg tggaacagga
     6061 aggtcctcag ggcaacgggg ctgaagtccg gcgacagggc gctcgatctc gcctgcggca
     6121 caggggccct cacccgagac ctcgcaaaga aggtcgggcc ggatggctac gtcttgggcg
     6181 tcgacttctc cagggagatg ctgcgcgcgg cccgcccggc gcagggcgtc gagtaccggt
     6241 tcggggatgc gaccgacctc cagggtgtgc cctccggcgc cttcgacgcg gccaccatag
     6301 cttacggggc aaggaacatc cccgacctgg acgcgctgtt ctccgagatg gcgcgtacgc
     6361 tcaggcctgg cggcgtcgcg gtgtgcctgg agatagcgcg gccgacgggc agggtatcct
     6421 ccgcgttcta cggggtgtgg ttcgataaga tcgttccgag actcggaggt tggatctccg
     6481 gtgacccatc agcctactct tatcttcccg aatccgtgaa agagttcgtg gcgcccggcg
     6541 aattaagtga cataatagag cgtaatggat tacaaaacgt tacatgggag cgcgtcgcag
     6601 gcggcatcat cacgctccat cgtggggtca ggccacacga cgcaggctag caggctccac
     6661 acagcgctgc ccgagggcgc cttcgagatc atcgacgagg tcgagggttt gctcgtgggg
     6721 gtggccggga acgcccctcc ggatctggtc gagtcgtcgc tcgaggccct tacgtcgggg
     6781 ggaaagcgtc tcaggccgct gctcttggtg ttgagcgcgc gcatggggga gcccaggctg
     6841 gaggacctgc tcggggcggc gacggccata gaggtgctcc acacggctac cttgatccac
     6901 gacgatatcg tggaccgggc cgagagcagg cgcggcgtgc cgaccaccgc cgccggatat
     6961 gggcgtgaga tcgcggccgc cacgggagat tacctgttcg ccgaagcttt ttcggagctg
     7021 gcaaggatca gggacccgag gctcgtgcgc gccttcgccg aagcctccgt ggggctcgca
     7081 gcgggggaac tagagcagtt ccgggcgaac ggggcgacgg tcgacgtcga atcgtacctg
     7141 gagcacataa ggaagaagac cgcgggcctc ttccaggccg cgtgcgtggc cggcggaacg
     7201 ctaggcgggc tctcactcgt gcagatagac gcgctcgcca gctacggcca ggcgctcggc
     7261 atagccttcc agatgtcaga cgacataatg gacctcgtcg gcaagcctgg ccttatgggc
     7321 aagggcatag gcacggacct cgttgagggt acggtaacat tgccggtcat cttcgccctg
     7381 agcgagggag acgcgggcac gatccggcgc gtcctagcag atcccaatcc gacgcctgaa
     7441 ctgctcgagg caggcataga agccgtgctc gccacggacg cggtggccag gaccgaggag
     7501 tgggccaggg gcgagatcga agccgccctc gcagatatcg gcctcctgcc cgttggtccg
     7561 gaacgcgaat tcctcgagtc cgtggcctcg gaggtggtcg ggaggaatgt ctgagagccg
     7621 ggcggagcct gggatcttcg cggccggaac cctgatgccc atctcgtcac ctccactgca
     7681 ggacggtgca gttctcgtga gggaaggacg cataggggcc gtaggagatc tcgcaaccat
     7741 cgggcacgaa aaccctggca ccgagatcag gttcttcccc aactgcacga tcatccccgg
     7801 ggcggtcaac gtccacgccc acctcggctt ccggcgcaag gatgcgccgg aggacggcgc
     7861 gttctcggag tggctgcgta ctctcataga gcgcctcccg gagaaagagg cctggacggc
     7921 cgaagcggcc ggcgacagtg ccagggaagc cgtagcagcg gggacgacgt atatggccga
     7981 gtcatcccct tacggagaat gcctgccgca gctcgccgaa agcgggatgg cgggaaccgt
     8041 ctacgcagag ttcttcccac atgagatagg cgccccaaag gaggccatcg actatatagt
     8101 cgccaaggtg agtgatcttc gagtaggtct accggagcgt gtcgacgcgc aggtgagcgt
     8161 acattctcca tacaccgtgg accctgagtc ctcgcgtctg gctgcgcgcc ggacgcggga
     8221 gatggggtgg aagctcgcaa tacacctctc cgagagcccc gaggaggtgg agttcctgaa
     8281 gagcggcacc ggcggcctcg cggacatctt cggttccaac gagtggggtg gaatcggcgt
     8341 ctcgcccgtc cgctacgcgc agggcgtaga gcttctcgcc ccccagacca tagccgcgca
     8401 cctggcgacg ggggtctccg atgaggacat agaggtcctg gcgcagaccg gcgtggcggc
     8461 ggcgcactgt ccccgttcca acgcctatct caggtgcggc gtctcgcccg tgccagccat
     8521 gatcgccagt ggcatcaggg tcggtatggg gacagatggg ctctggagca gcccgagcat
     8581 gaacctcttc gaggagacgc tgttcgcggt ggagttgcac ggcatgagtg gtgcggcagg
     8641 cctcgagctg gccaccctgg gaggggctcg ggcgctcggt atcgaggccg gaacggggac
     8701 gctcgaggcg gggaagtggg cggacttcgc cgtggtcgag accctggggg agggcagaga
     8761 ccccgagctg ggcgttctcg aagccgccgc cggcggggga gtcgcggcca cggtcgtcgg
     8821 tggtgacatg atatacaatc gtctcggcac ctgaagattc cggaggacag accgccatga
     8881 tgatgaaccg cgacaaactc agtttctggg tgaggttggt ctccatcgtg ctcgcggcgt
     8941 tcttcctcct ctccttcatc tttctcggcc ttgggaccaa catcagctac aacctcttcg
     9001 agctgatcgg caaccaggac caacagcaac agcaggccga ccaggccccc gatccgcagg
     9061 agcagatcgc ggatgccgag aaggccctgg agaaggaccc caaggacccc gagaagataa
     9121 aggacctcgc cgccctctat tacaccgcgg gccgctacga cgatgcgatt caggtactgc
     9181 agcgagggcg cgaggtcgcc ccgcaggacg aagagatccc gaccctcata ggtcagatct
     9241 acagccagca ggcacaggcc gagaaggggg agaagcagaa ggagcttctt aagaaggcgg
     9301 gggatgcctt cgccgcttca gccgcggcgg agccggacaa cgcagaagtc tacctgctgg
     9361 ccgcgcaggc ttatgaccag gcaggtcagc ccgccgatgc catcgaatac tacaacggct
     9421 acctcgacct cgagccgaag ggcgagaacg ccaagcaggt gaaggacagg atctccgccc
     9481 tcctcgaagg cggtggcggt agcgctgggc cctgatccgc atcagtaagg aaggcgtata
     9541 atgtggcgtg agatccagca gatagctcgt taggaggaaa ggtgtttgga tcggtcggaa
     9601 tgccagagtt gatcataggt cttgtcgtca tactcctgct cttcggggcc aagcgcgttc
     9661 ccgagcttgc ccgcggcctc ggcagcggcg tgcgcgagtt caagaagggt acgcaggagg
     9721 gtgagatcga ggacgagaag aaagaggaag cgaagaagga cgaagaacag ctcgaagcaa
     9781 gcaagagcga cgacgagaca aagctggagg aggatgcttc cgtcgaggcc gagaaagccg
     9841 agcagaagca tagctagacc ccttacgggt tcctaagctc cacatggcct ctcgcctgag
     9901 actcccttcc cgtccgcgcg acgagatgcg tatgaccctg atcgagcatc tcgacgagct
     9961 caggtccagg atcatcaagg tcggcgttgc gttcttcggc gtcgccatcg tcgcctggtt
    10021 tttccggcag cggatcttcg aatggctact cgcccccgcg ggcggggccc tacaaggcaa
    10081 gttgaacgtc acaggggtta ccgagcagct gttcaccgac atgaaactcg cactctacgt
    10141 cgggttcctg ctcacgatcc cggtactcct ctaccaggtg tgggcgtttg tggcccccgc
    10201 ggtcggcgac atgggccggg tcttcactta caccataatc agcatggcct ccgcgctgtt
    10261 catagccggg gtggccttcg ggtacttcat agtattgcct gtcggcaccc aattcctgct
    10321 caactgggac ccggagcgct tcgatcccat cattaccgcc cagagctatc tgcctttcgt
    10381 gacgaggttt cttttggcct tcgggatagt cttcgagtta cccgcggcaa cgtacgtcgc
    10441 cgcgaagctg gagctcgtcg acgccccgct tctcaagagg tacaggaagc acgccatcat
    10501 cctcaacacc gtcctcgccg ccgccctgac gcccggacag gacccctaca gcatggtcct
    10561 gatggccgtc ccgatgatag tgatgtacga gatctccata ctgatcgccc gctacgtgaa
    10621 cccgacgaca caggtcacgg cacacgaact ggtaagggac gatggggatg agtccgaaga
    10681 gcccgagccc gatgagcccg atatagatga cttcgaagac acagaagatc accccgagaa
    10741 cgggcacgag cgggacctct agccgtacct caggtgcgcg tcgaagcggc gcatcgagcg
    10801 cctgagcagg tcctcggtaa gcctgcggaa gaaaggcttg tgggccaact tgtcgagcca
    10861 ggctggtctg atcaggtagt ctgtcgagag ggttatcctg atggcaccgg gggcctcctg
    10921 ctcgacgagc cagccgccgc ttccagtggc gccttcgacg tagttccatt ccagccggcg
    10981 tggcggttcg accaccgtga cgatgctcga tacctcgtgc gtgagaccgg cggcgctcag
    11041 ggtaaactcc agcctgacgc cggggcgtaa aactttcgtc tctactttgc cggcagggtc
    11101 gagcacgcgg cagcgccgga ggccggccgt ccattcgggg aagcgttgca catcgaggag
    11161 ggagtcgaag atcctctccg ccgggacccc ggatagaacg tagctcgcct tgaaaggcct
    11221 gtgtctctca ggcaccgatg aggactcccg ccgtgtagag caatccgaag acgaggtgga
    11281 gcccggcggt ccgcttgacc acggggtcga ggcgactcgg cgtcgagtag ctggccacgt
    11341 cgcgccagag gcggagggcg gcgaacacgc tcgggaagac gagggcacac tctgggggga
    11401 cgacgccgaa ggccataagc acggcgaccg cgacgtaagc ctcgagcaag agtgcccggt
    11461 acacgaggat cgcgacctcc ctgccgaaga ccacgggcag cgtccgccgc ccgcccctac
    11521 ggtcggagac gagatccctt atgttgttgg cgagcaggat cgcggccacg ggcccaccga
    11581 taggcagcgc cgccagcggc acgtacacgg gaaaaccgcg agcctgcacg gcgtaggcga
    11641 tgacgacgat caaaaccccc atgaagacga acaccgcggc ctcgctggcg ggggtgtagg
    11701 cgatggggcg cggtccggcc gagtagaggt ggccgccgag cgccgagaga cacccgagca
    11761 cgagtatggg ccagccgcca acggctatca ggtagatgcc gaaaaataag gccagggtgt
    11821 agcaaagcag cgcacccgca agaacgctgc gcgaactcac ccgaccactc actatagagc
    11881 cggcgatccc gaccgtcccc gctacgtcga ggccgcgctg cgcgtcgtag aactcgttga
    11941 acatgttggt ggccgcctgt atgaggagac ttccgaagag catggcgaag aacggccccc
    12001 aggcgaacgt gccgtccttg aacgccagcg cggtcccgat gagcacaggc accgccgccg
    12061 cagtcaggct gaaaggtctg gcaaggcgga accactcttc gaaagcctgc cgtctcgtcg
    12121 aagtcgccat tcggccaatt ctggagagag tcgtcggcgt ttgcaatcgg tccgttcacg
    12181 gttttcgaag cattaggcta caggcatcag gcttcaggta ggtttgctag tggcatcttc
    12241 gcgggttctc cacacgccct ttcttgcagt gcctcgggga taccaggcgg tgcggcaacg
    12301 ttgccccgtt gctccctgaa caaaccgaaa cctgtagcct gaaacctgat acctgatagc
    12361 tcaaacctga aacctgacac ctattgtgat acaaccggca gtcgtggaag ccggcagttc
    12421 cagtggactc gcgagctctt atctgcggct ggtcaggccc aggcagtgga ccaagaacgg
    12481 gttcgtgctg gccggggtgg tcttcgcaga gaaggctctc tcggcgaccg cgttggccga
    12541 cgcgctgctc gctttcgcga tcttctgtgc cctctccggg gccgtgtacg ccgccaacga
    12601 cgtgttcgac gtcgaggagg accgcaaaca cccactcaag aggttcagac ccgtcgcgag
    12661 cggtgagatc tccgcccgcg cagccctgat cttcgccgcc gtgcttgcgg caggtgcgct
    12721 cgccgtgagc tttgcccttt tgccttacgg cgtcgggatc gccggtattg catacctcgt
    12781 cttgcaggtc ttctatacga ccatcctcaa gcatgcggcg atcctcgacg tcatgagcat
    12841 ctcggccggc ttcgtcataa gggcgctggc cggcgtggcc gccgtatcca gccctatctc
    12901 accgtggctc atcgtatgta ccggcctcct tacgctcttc ctcggcttct ccaagcgtag
    12961 acacgagatc gctacgctgg gcgaaggggc ggtcgtccac cgcaagaatc tccaggacta
    13021 ttccgttccc atgctcgacg aaatgatgaa catcatgatc tcggcgacca tcatcgccta
    13081 taccctctac accttcacgg tctacgagag gcaggatcag atcttcatga tggggagcat
    13141 cccgttcgtg gtatacgggg tcttccgcta catgttgctc gtccaccgcg acggcggcgg
    13201 caaccccgac accctgctcc tctccgacag gccgctccag atctcgctgt ttctctggct
    13261 ggtcgtagta gggacggcca tctacgtaat acccctgtgg aacggctgag acgaaatctc
    13321 gtcctcgccc tcgccctggg cgccgccgtc tacctgatcc tcgccgttct ctccgacctc
    13381 ggcgagctcg tcgcggccct cgacagcttc gactacgccc tcataccagc gatcctcggc
    13441 ctcgtcgccc tctcttacgt cgtgcgcttt ttccgctgga cgtactacct cagggtactt
    13501 aaggtctccg ttccaacggg gataaacgcc gcgatcttca cctccgggct ctcgatgacg
    13561 atctcaccgg gcaaactcgg tgaggtgctg aagagcgtct tcgtccgcga cgccacggga
    13621 accccgatag cccgcacggc ccccgcggtc gttgccgaac gcgccacgga cggtacaggc
    13681 atggtcctgt ggggtttcct tggagccttc gccctcaccc ttcccccctg gacgatgctg
    13741 atgttcctcg ccctggcagc cttcgggatc gccgtcctgc gctccaaacg cctctctctc
    13801 gcggccgaaa gcgtcctatc gaagctgccg ctcctgaacc gcctcgcccc gcacctgagt
    13861 gtcttccacg gagcatcgaa cgagcttctg ggaacgagac cgctcctggt ggggaccgcc
    13921 atctccttct tctcgtgggg cctggagtgt gccggcgtgt atctgtgcgc ggttggcctc
    13981 ggcgtggacc ggccattcct gctcatcgtc ttcgtgttcg cgaccagctc gatcctcggg
    14041 gtccttagca tgctccccgg aggactcggc gcggtcgagg ccggtctcta cggccagttc
    14101 gtcgccgtgg cgaacctctc gacgggggtc gccggcgcgc ttacactggt gatccgcctg
    14161 gcaaccctct ggttcgccac actactcggg atctgcggcc tactcctggt cagacgcctg
    14221 ctcggcgacg cgtccataga accggataag acataaccga ggaaggcttc cctactaaac
    14281 catcgtccct ctaacgatgt agcgtgcgta ccagttcgga ccgggagtcc agtagtcccc
    14341 gtagtcctcg atgcacgtct gcaaagtgac cacatcgccg ccagttggtg cggttacgga
    14401 tagatccgtg ggtgacactt ccaggatctc gctcacagcg tacgtgtacg tcgtgccgtc
    14461 ggaggcggtg agcaggatcc ggtctccctg acccagattg ggcaggttcc agaacacgtg
    14521 atcgctcgcg gtgccagggt atcctatcct gtgaccggct atgtaggtgt tggagccggc
    14581 aacccacggg tagccggtgc caggaaggtg cccagccccc tgcgagaggc tcgcctccga
    14641 tgtcccctcg accacgggaa tgtccgagat acccatagca ggcacactca aagacatcag
    14701 cgcgcctgca gggacggtag ccggtggcga agcagacgcc tcgttctcgg gctcggtctc
    14761 ggcgacaggc tccggtgtgg gcgcctccgc cccactaggc tggatggccc tctcggcagg
    14821 ctgggcgtca gcccccgcaa tcatcccacg accggccaga gcgagcgcgc cgatagcaag
    14881 catcgcaatg acagcacacg taaagatgcc aaacctgccg aggccagtac cgaaaaaacc
    14941 cctcataacc cgtcctctac ttcccccgaa tcagaggcta tctacgaccc ccacccaaca
    15001 agcccaaaca cgtcagccgt gacccgccgc actctaacac cgccgcgcat gaacagcaag
    15061 aatcttaaca aacttgtaac acgtcgttct acgagcgact tgcagagatc gacggctacc
    15121 atcgatcagc gagaacagaa cgctgagacg cttctctagt ccgcttctcg catcgtgcgg
    15181 ctcagtgctc ccaacacggc cgttagtagc agtagcgctc ctgcggccag gaacatagac
    15241 gttagatcta gctcgacgag gatcccggca agggcgaagg agatcgggtc cagggcgacg
    15301 gcg
//
LOCUS       DATMVF010000080        16275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_150663, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000080 DATMVF010000000
VERSION     DATMVF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150663"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VN178_03515"
     CDS             <1..1310
                     /locus_tag="VN178_03515"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWS80068.1"
                     /translation="DEELVAFLHCDISGLNRGRSVPAAELDERFAAGVGWVPADQALT
                     PFGPIAEPNPFGSLGDLRLLPDRDTRVRVDLWDGVSPLHFYFCDAVNTDGSPWDACPR
                     TLLKGALSRLEEAGFRLVASFEQEFILEGLGSEPGPGFSLEAQRLVEPFGPLVMAGLR
                     QAGLEPEVFLPEYGEGQYEVPCAPAEGVSGADRAAAMREIVREVARRLGYRASFVPKA
                     DPEGVGNGVHIHLSLLDLEDRPVFYDPERPGRLSTVGARFAAGVLEHLPALCAFTAPS
                     VVSYLRLVPHFWSAGFGCLGYRNRETAIRIPPIVELSGGDPARQLNLEFRAADAAACP
                     HLALAVLVLAGLRGLRDELPEPILIEDDPTGLDEEERRRLGISELPGSLEEALGALES
                     DEVVRSWLSKDLLDCYSSVKRNEIDLLEGTEPWEACERYLRVY"
     gene            1285..2499
                     /locus_tag="VN178_03520"
     CDS             1285..2499
                     /locus_tag="VN178_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWS80069.1"
                     /translation="MSGTCVSTDLLETLLHSLEEELPRAFELREKLHAIPEPSHEEHA
                     TSSLVAEALGTGNMKSVARTGLLAHVGPPGEAIAVRAELDALPIQEETNTSFAATNGF
                     MHACGHDVHMAALVALFRAAGRIEEPLPKPLVALFQPSEEAYPSGADLVVRERRLEEE
                     TGAVVAAHVHPDVPWGSVSVEAGPVNASSDNLRITVEGSGGHGAYPHRAHDPILALSH
                     AVVALQSLVSRRLDPTHAAVFSVGWMRAGSAENVIPGVAEAGGTLRVLDPEDREPLRK
                     IAREIVDNTARAHGCAARIEVTEGEPATVNDPALAEAANSLLTEAGFEPAPPMRSCGS
                     DDFGFYGRTSPTLMLFVGIEDGPHTPNVPLHHPRFLPPGEAIGAVARAQAVAFTAAAT
                     SLDRVPLGSDGG"
     gene            2525..3691
                     /locus_tag="VN178_03525"
     CDS             2525..3691
                     /locus_tag="VN178_03525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWS80070.1"
                     /translation="MEALDFTQFPVVDNHCHGIMRDQVFEDITGWRQAFTESSDPGMA
                     RGHIASTAFYRRLIRSLADFLDCEPGEEDVFAARTKRDGRELAGALLRAANVDTLLLD
                     TGFPPPEEVLPVPELGEIGGCRTEPMLRLEILMESLLAEHDSLEDVKEALVAELDDVR
                     GRGYVALKSIAAYRTGLDIREWPREEAEESFREYRRAAGAGTARLVHKPLLDTLLHVA
                     FARAARQEVPIQFHVGYGDADTDLLLGNPLYLRPVLQRPDYRGMPVVLLHECYPYTRQ
                     GGYLAAVYQNAYLDLSYGIPLLGYGEMLSFTRQAFGVAPSSKLMYSSDGIGVPELHWI
                     SAIDGRRVIGEALGEMVAQRDLVLPEAEAVGEGVLRGNAARLYGLKADKRSPKG"
     gene            3873..4346
                     /locus_tag="VN178_03530"
     CDS             3873..4346
                     /locus_tag="VN178_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80071.1"
                     /translation="MYRQARLLGALLILQFLAMVGLGVWLTTRVNWDRALDITSGQGQ
                     FNFQSVEGPWLKALERSIVFDVYFLVPMILLLLSGLGFVLLRRRGWMLASIAQSLIML
                     ACLFYYGDSRPWFAYPLIAYSILMILLLNSRSVRAAVQSRRAAPVGTRSEVAHGE"
     gene            4336..6192
                     /locus_tag="VN178_03535"
     CDS             4336..6192
                     /locus_tag="VN178_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80072.1"
                     /translation="MANEGSVDARTAEELLRRFEPVIRFTKGEWFYPMDCEPYVDACS
                     LWVARPNEDAVCVVPAGELTLERLAQQPEDTTGAVHYLKIPNPKESENGGRRRKRRSH
                     TNPDWPKETFRAGRGRLARVGYISRLADALFSLTLLARGRVPGGDVEGGRVLYGRYME
                     EGQRFRYHGNVVRQDGWIVLRYWLFYAFNDWRSNVFGANDHEADWEKIFIYLSESESG
                     EVRPEWVAYAAHEETGDDLRRRWDDPELEKIGEHPVAYVCAGSHATRYFRGEYLTELA
                     FPLPRFLSAVRRIARRFWYVTLRQYADEDYKEKPYEQFTIPFVDYGRGDGWSVGPGQD
                     REWDPPRLMNPVPSWVSGYRGLWGLYTRDPFEGEDAPAGPMYNRDKTISREWFDPVGW
                     AGLDKVPTRAEELEVITGRQAEIEERQDFLRSELREKSSRLKTLGMDAEATRDQSHLT
                     KLHETQTRHIEELSREVEALREELSDDEVLAETLKSHAESLRSGERPPPHAHIMHATR
                     PVSEAETHAGRVAQLWASISIALVLVTLILIIAYERSHLLSALVFAIAVFAFIEASFR
                     RRLARFIVSVNVALAVVGALVLFEQYFWSVIVFAVLGIGFYILWENLRELGR"
     gene            complement(6236..6505)
                     /locus_tag="VN178_03540"
     CDS             complement(6236..6505)
                     /locus_tag="VN178_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWS80073.1"
                     /translation="MARSHKVTFKQSGITIDVAEDEYILEEAEHAGLHLPYDCRSGTC
                     TTCIQKCLEGEIDQDMAFAIGDEELEQGLRLICIGSPLSDVVLDA"
     gene            6769..6945
                     /locus_tag="VN178_03545"
     CDS             6769..6945
                     /locus_tag="VN178_03545"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS80074.1"
                     /translation="MLLKKVYRFRMRPTKAQEAALYRMAGARRFVYNWALERRKSYYT
                     EHGKGISARVLPPS"
     gene            6942..7940
                     /locus_tag="VN178_03550"
     CDS             6942..7940
                     /locus_tag="VN178_03550"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HWS80075.1"
                     /translation="MTAIKGEPDTHWLKEADSQMLQQALKDVKRAFDAFFEKRSRFPR
                     FRSKKAGHFTFRIPQRVKVKEGKVYIPKVGWVRIRQSRALEGTTKSATFKRDACGHWY
                     VALTTEFEIPVASLEAPATAKPVGVDLGLKDLFVLSDGGRVPAPRFARKGSRKLREAQ
                     KGLSRKKPGSRRREKAKQRVAKVHRKVPNQRSDFLHKLTTGLRRKYDCICVEDLSVKG
                     LAKTKLSRSIHDAAFGEFVRQVEYKAAWNLRHSVRVGRFFPSTKLCSECGALNPNLKL
                     SDRTWLCVSCGTHHDRDLNAARNILAEGLRLVAAGQTETVNARGEPVSLPKGSTVR"
     gene            complement(7975..8655)
                     /locus_tag="VN178_03555"
     CDS             complement(7975..8655)
                     /locus_tag="VN178_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS80076.1"
                     /translation="MLIRVLAVLGVGVWCFFILIVSVAFVIDRGLADVGIVWLAIFGL
                     GVVAPVLLLGQINRVIAPHETSRGAFLGVRRKEQEILGALEEGGSLTPAAAAMRTSLT
                     VDEASKMLDGLARKGYLETQTEGGIVAYALAGHRSQELPGVDTALRREQPGVNGASHR
                     LEDPLSERELEVLALLASGKTNSEVAGDLFVSVGTVKSHTGNIYRKLGAKNRAEAVTV
                     ARNLGLIR"
     gene            complement(8921..11236)
                     /locus_tag="VN178_03560"
     CDS             complement(8921..11236)
                     /locus_tag="VN178_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS80077.1"
                     /translation="MYKEIYVPVDNSDYSNQACVIGVDVARQFGGRVAGCHAYAAKMH
                     DVRFRQMESGLPEEFRDEDEMKRQRKIHDQLITKGLEIITDSYIDVLEPLCEKYDVEL
                     VRRSLEGKNFKVIVEDVNENDFDLVVMGAMGMAAVKDTVLGTVTERVVRRLQKADTLI
                     VKDLDRSPFEHIVVAVDGSAKSLGGLKRAIELAGEFGGTVEAISVFDPYFHYAMFHSI
                     AGVLSTKAQKVFRFKEQEKLHEEIIDSGLAKIYTAHLEIARKIAEDEGVDLKTTLLAG
                     KPFEQTLKYVNEVNPTMVVMGRIGYHSDDEMDIGGNTENMMRYLPTNVLITNYEYQAP
                     DLYTAEEHMTWTKEALERLDRIPGFVVGMATGAIIRYAIEKGYTVITTGVIDEVIKSI
                     LPPGAMESMRAIGDQMRAAEAMGEDKPIDSLFKDFDERTAGKANGTNGHNKGANGSDA
                     AAKQEIFDAGRGGFGKSEDQGDIEGVSAEVQDEIRRSAQGQDRFECDVCRYVAKGKPA
                     KCPVCGSGPTHFHAVDAEIATTSEDENLNLSEVYDGRELHWTDDATALLDSLEEWQEK
                     RRVKARVEKSALKKGYTTITREYVEQQYREETGREAPDVKSGGCPVSHAKEQVQETTG
                     AKCPIDHSAFQKAGASVPEGGSTEFTWTDDAIERLERAPKGFMRNISRNMTEKLARER
                     GTTNIDLDLVEDSLSGARTTMEDVITGKVSIADLARDAGERIEAEDGEAPHPPMTVTM
                     VCTTCGEELQGVQPPEECPVCGAPGEDFEQK"
     gene            11482..12420
                     /locus_tag="VN178_03565"
     CDS             11482..12420
                     /locus_tag="VN178_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWS80078.1"
                     /translation="MGLEKMEQDVRAKDHGSYAEGAAFVEGRFVPIAEARVPILDWGF
                     LRSDATYDVAHVWRGSFFRLEDHIDRFERSMEHLRLSLPYDRNEIRDILIECVRLSGL
                     RDAYAEIICTRGIPRPGSRDPRDCDNRFYAFVVPFIWIADPEKQEQGLHAVIAGKQRI
                     APESVDPTVKNYHWLDLETGLLESYERGGETVILVDAEDNVVEGPGFNVFAVKDGTIS
                     TPARGVLEGITRKTIIELAAEHGIPLEARPVPADELRQADEVFLSSTAGGIIPVTIVD
                     SEAVDNGKPGPLTRQLRAAYWELHDDPRFSLPVRYD"
     gene            complement(12477..15167)
                     /locus_tag="VN178_03570"
     CDS             complement(12477..15167)
                     /locus_tag="VN178_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWS80079.1"
                     /translation="MKTGEKETVGEGLVLPFEDLDRSALPMAGGKAANLGELTRACLP
                     VPPGFCVTTAAYELVAKGAGLGGILDDLATTSPDDAARLAELAANARDTLLAAPVPPD
                     VAGTIATAYRAFGDGEAVAVRSSATAEDLPSASFAGQQDTYLNIVGEESLVEAVRCCW
                     ASLWTDRAVSYRATNGIDPRSVRLAVVVQKMVEAEVAGVLFTANPITGRRRQAVIDAS
                     FGLGEAVVSGAVNPDHFVVDTVTGEILERRLGDKRATIRADKDGGTRRVELEKDEEPS
                     LTDTQIRALAKLGARVEELYGEPQDTEWAIDSSGKIWLLQARPITTLFPLPEGASTND
                     EDLRVYFSFSVAQGVYRPLTPMGIQTFRLITSAVATMAGRPPRNPYKGAAFFTEAAGR
                     IFADITPVLRSKRGRGLFEWAMRNMEARTVAIVRHLAADPRLAPVPTPMRPVLDSILT
                     VFVRGRIPLRVVEALARPGKARARAARVEGQFRAAEDVPHDAAECLAAAERLLLDGPP
                     DLLPNVPPVFAAGLAANALAGKLLGGLATDNERRVAMRALPHNPTTEMDLALWSLAKE
                     ARADPDVARMLGETPPERLAEDYRLGSLPPKLQKSLADFLRLYGHRGVAEIDLGLPRW
                     SEDPTYILSVLANYLRLDDPEAAPDVQFARATREAEQMVGELTRRATRKGRLRGALVR
                     FLLGRARALSGLREMPKFCIILLMARARELLWTAGAELAEAGRLQSAGDIFFLSMPEA
                     WAALAGEDLHPLVRDRRAVYEQEIDRRHVPRVLLSDGTESTAGTHHATGADGVLRGTP
                     ASGGVVTQKARVILDPGEAGLEPGEILVAPSTDPGWTPLFLPAGGLVMEMGGPMSHGA
                     IVAREYGIPAVVGVPDATELIETGRRITVDGSAGEVTPEK"
     gene            complement(15249..>16275)
                     /locus_tag="VN178_03575"
     CDS             complement(15249..>16275)
                     /locus_tag="VN178_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS80080.1"
                     /translation="LLELRHSEVRNFTLQTASGPFTPIDFGRLKTKYPYITIMPQTSF
                     LEFVTQEAERYPGFRLLMRARVRELVEEDGVVRGVRYESEDGRHEVRAVLVVGADGRG
                     SRVRRLAGFEPMKTSPPMDVLWFKLPREKGDPEGLQGRFGRGHIAIMLDRDDYWQAGY
                     VIPKGTYPGLRREGIDTLHRGFGELIPEFADRVEYIEDWSQVSLLSVESSMCPRWYRP
                     GLLLIGDAAHVMSPVAGVGINYAIQDAVVAANVLIRPLKASQAQLRDLDVKHLAKVQR
                     RRELPTRMIQRAQALIQQQVLARALRSNEPFAPPLPLRLFLRVPILRDIPARIVAFGF
                     WPVRVKE"
BASE COUNT         2787 a         5286 c         5261 g         2941 t
ORIGIN      
        1 gggatgagga gctcgtcgct ttcctccact gcgacatctc gggtctgaac cggggccggt
       61 cggtcccggc cgcggagctc gacgagcggt tcgcggcggg cgtagggtgg gtgccggcgg
      121 atcaggcact caccccgttc ggacccatcg ccgagcctaa cccgttcggg tcgctcggtg
      181 acctccgcct cctccccgac agggacaccc gcgtgcgcgt agatctgtgg gacggcgttt
      241 cgcccttgca cttctacttc tgcgacgccg tcaacaccga tggtagtccc tgggacgcct
      301 gcccgcgtac gctgctcaaa ggcgccctct cccgtctcga ggaggccggg ttccgcctgg
      361 ttgcctcctt cgagcaggag tttatcttgg aaggcctggg ctcggagccc ggaccaggct
      421 tctcgctgga ggcccagcgg ctcgtcgagc ctttcggacc gctggtgatg gccggactgc
      481 gtcaggccgg tctcgagccg gaagtgtttc ttccagagta cggagaaggc cagtacgaag
      541 tcccctgtgc acccgcggag ggtgtctcgg gtgccgaccg ggcagctgcc atgcgggaga
      601 tcgttcgcga ggtggcccgt aggctcggct accgggcgag cttcgtcccg aaggccgacc
      661 cggagggcgt aggaaatggc gtccacatcc acctgagcct ccttgacctg gaggaccgtc
      721 ctgtcttcta cgatcccgag cgaccaggcc ggctcagcac cgtcggcgca cgctttgcgg
      781 cgggcgtcct cgaacacctg ccggccctct gcgccttcac cgccccgagc gtcgtctcct
      841 acctgcgcct cgtgccgcac ttctggagcg cgggtttcgg ttgcctgggc tatcgcaacc
      901 gggagacggc catccggata ccgccgatag tagagctgtc gggcggcgac cccgcgcgcc
      961 agctcaacct ggagttccgg gcggccgacg cggcggcgtg cccccacctg gccctcgccg
     1021 tgctcgtgct cgccggcctg cgcgggctcc gggacgaact ccccgagccg atcctgatcg
     1081 aggacgatcc caccgggctc gacgaagaag agcgaaggag gttgggcata agcgagcttc
     1141 caggctcgct ggaggaagcc ctcggagccc tcgagtccga cgaggtcgta cgctcctggc
     1201 tctcgaaaga cctcctcgac tgctactcga gcgtgaagcg gaacgagata gatctcctgg
     1261 aaggaacgga gccctgggag gcctgtgagc ggtacctgcg tgtctactga tctcctcgag
     1321 acgctcctcc attccctgga agaggagctt ccgcgagctt tcgaactgcg cgagaagctg
     1381 catgcgatcc ccgagccgag ccacgaggag cacgctacct ccagcctcgt cgcggaagcc
     1441 ctcgggaccg ggaacatgaa gtccgtcgcc cgaacgggcc tcctggcgca cgtcggacca
     1501 ccaggggagg ccatcgcggt gcgggccgaa ctcgacgcct tgcccatcca ggaggagacg
     1561 aacacatcct tcgccgccac caatgggttc atgcacgcct gcggccacga cgtccatatg
     1621 gccgccctcg tagcactgtt tcgggcggca gggcggatcg aagaaccctt gccaaagccc
     1681 ctcgttgccc tcttccagcc gagcgaggag gcttaccctt cgggagcgga tctggtcgtt
     1741 agagaaagga gattggagga ggaaacgggt gcggtcgtgg cagcgcatgt ccacccggac
     1801 gtcccctggg gctcggtaag cgtggaggcc ggcccggtaa acgcttcctc ggacaacctg
     1861 cgcataaccg tcgagggatc cggcggccac ggtgcctatc cccaccgcgc acatgatccc
     1921 atactcgccc tctctcacgc ggtcgtcgcg ctccagtcgc tggtgagccg caggttggac
     1981 ccgacgcacg ccgcggtctt cagcgtcggt tggatgcgtg caggctccgc cgagaacgtc
     2041 atcccgggcg tcgccgaggc cggaggtacg ctgcgcgtcc tcgatcccga ggatcgcgaa
     2101 ccgctgcgaa agatagcccg tgagatcgtg gataacacgg ccagggcaca cggctgcgct
     2161 gccaggatag aggtgaccga gggagaaccg gcgacggtaa acgaccctgc cctcgccgaa
     2221 gcggcgaact cgttgctcac cgaagccggg ttcgagccgg cgccgccgat gagatcctgt
     2281 ggatcggacg acttcggctt ctacggccga acgtcaccga cgctcatgct gttcgtaggg
     2341 atcgaagacg gtcctcacac cccaaacgta ccgctccacc atccgcggtt tttgccgcca
     2401 ggagaagcga tcggagcggt cgcccgcgcc caggccgtcg ccttcacggc tgccgcgact
     2461 tcccttgacc gggtccccct cgggtccgac ggcgggtaag tgatgctttg aacggaggtg
     2521 gcgcatggaa gccctagatt tcacacagtt ccccgtcgtg gacaaccact gccacggcat
     2581 aatgcgggat caggtcttcg aggacattac gggttggcga caggccttca ccgagtctag
     2641 cgatcccggc atggcgcgag gtcatatcgc gagcacggct ttctaccggc gcctcatccg
     2701 gtctctcgcc gacttcctcg attgcgagcc gggagaggaa gacgtcttcg cagccaggac
     2761 taagagggac ggacgggagc tagcgggtgc gttgttgcgc gctgcgaacg tcgacaccct
     2821 cctccttgac acgggtttcc ctccgccaga agaggtgctg cctgtaccag agctgggaga
     2881 gatcggaggc tgccgcacgg agccgatgct gaggctcgag attctgatgg agagtcttct
     2941 cgcagagcac gattctctgg aggacgttaa agaggcgctc gtcgccgagc tggacgacgt
     3001 gcggggccgg ggctatgtgg cactcaagag catcgccgcc tacaggacgg gcctcgacat
     3061 tcgggagtgg ccccgcgagg aggccgaaga atcgttccgg gaatacaggc gggccgccgg
     3121 agcgggcact gcgcgtctcg tccacaagcc gctcctagac acgctcttgc acgtcgcttt
     3181 cgctcgggcg gcccggcagg aggttcccat ccagttccac gtcggctacg gtgacgcgga
     3241 caccgatctc ctgctcggca acccgctcta cctgcgtccg gtgcttcagc ggccggacta
     3301 tcgtggcatg cccgtcgtgc tgctgcatga atgctacccg tacaccagac agggcggcta
     3361 cctggccgcc gtctaccaga acgcctacct cgatctctcc tacggcatcc cgttgctcgg
     3421 ctacggcgag atgctctctt ttacgcggca ggcgttcggc gtcgccccga gctccaagct
     3481 gatgtacagc tcggacggca taggcgtgcc tgagctacat tggatcagcg ccatagacgg
     3541 caggcgggtg atcggcgagg cgctcggaga gatggtggcg cagcgagatc tcgtcctgcc
     3601 cgaggccgag gctgtaggtg aaggtgtgct tcgcgggaac gcggctcgcc tctacggact
     3661 caaagctgac aagcgaagcc cgaagggctg agcgtcctga caagccgcct ggcgaagccg
     3721 ggcggcgtgc tgacgagcgg aggccgcagg ccgaagcgtg ctgacaagct ggcgtagcca
     3781 gcgtgctaga aaggtgcgtg aacgggctaa agcggttatc ctatcctcca ggggtgtagt
     3841 ctaggctcgt aacccgaccc gagaggctgg cattgtaccg acaggcacgg ttacttggtg
     3901 cgctgcttat tttgcagttc ctcgcgatgg tggggctcgg cgtctggttg actacccggg
     3961 ttaattggga ccgggcgctg gatataacaa gcggacaggg ccagttcaat ttccagtccg
     4021 tcgaagggcc gtggttgaag gctctcgaac gatcgatcgt gttcgacgtc tactttctcg
     4081 tgcccatgat cctgttgctt ctctcaggcc tcggcttcgt gctacttcgg cgccggggat
     4141 ggatgctggc ctcgatcgcg caaagtctta tcatgttggc ttgccttttc tactacggcg
     4201 actcgcgtcc ctggttcgcc taccccctca tcgcctacag catcctgatg atcctgctcc
     4261 tcaactcccg gagcgtacgt gccgcggttc aatccagacg ggcggccccg gtgggaaccc
     4321 gatcggaggt cgcacatggc gaatgagggg agcgtggacg caaggactgc cgaggagttg
     4381 ctgcgtcgct tcgagcccgt gatccggttc accaaaggcg aatggttcta ccccatggac
     4441 tgtgagcctt acgtcgacgc ctgcagcctc tgggtcgcgc gccccaacga ggacgccgtc
     4501 tgcgtagttc cggccggaga gctcaccctg gagagactcg cccagcagcc ggaggacacc
     4561 accggcgccg tgcactacct caagatcccc aaccccaagg aaagcgagaa cgggggtcgc
     4621 cggcggaagc ggcgctcgca cacgaacccc gattggccca aggagacttt ccgggccggc
     4681 aggggccgcc tcgcgcgcgt cggctacatc tcgcggttgg cggacgcgct cttctccctg
     4741 acgttgctgg ccagggggag ggtccccggc ggggacgtgg agggggggcg cgtcctgtac
     4801 ggccgctata tggaggaggg acaacgcttc aggtaccacg gtaacgtggt tcgccaggac
     4861 ggttggatcg tgttgcggta ctggctcttc tacgctttca acgattggag gagcaacgta
     4921 ttcggtgcca acgaccacga ggccgactgg gagaagatct ttatctacct ctcggagtcg
     4981 gagtccggcg aggtaaggcc cgagtgggtg gcctacgccg cgcacgagga gaccggcgac
     5041 gacctcaggc gccgctggga cgacccggag ctggagaaga tcggcgagca tcccgtggcg
     5101 tacgtctgcg ccggctcgca cgcgacccgc tacttccggg gcgaatacct gacagagctg
     5161 gctttccccc tgccgcgttt cctctccgcg gtccgtagga tcgcgcgtcg cttctggtac
     5221 gtaacgctgc gccagtacgc cgatgaggac tacaaggaga agccctacga acagttcacc
     5281 atccccttcg tagattacgg gcgcggggac ggatggtcgg tcggaccggg gcaggacagg
     5341 gaatgggacc ccccgcgctt gatgaatccg gtgccatcgt gggtctccgg gtaccggggg
     5401 ctgtggggac tctacacccg ggatcccttc gagggcgagg acgccccggc ggggccgatg
     5461 tacaaccgcg acaagaccat cagccgcgag tggttcgacc ccgtgggctg ggccggtctc
     5521 gacaaggtgc cgacccgggc ggaagaactc gaggtcataa ccgggcgcca ggcggagatc
     5581 gaagagcgcc aggatttcct ccgctccgaa ctacgggaga agagcagccg gctgaagacg
     5641 ctcggcatgg acgccgaggc cacgcgggat cagtcccacc tgaccaagct ccacgagacc
     5701 cagacgcggc acatcgaaga actatccagg gaagtcgagg cgctgcgcga ggaactgtcc
     5761 gacgacgaag tcctcgccga gaccctgaaa tctcacgccg aaagcctgag aagcggcgag
     5821 cgtccccccc cgcacgccca cataatgcac gccactcgcc ccgtctcgga ggcggagacc
     5881 cacgctggga gggtggcgca gctgtgggcc tcgatcagca tcgccctggt gctcgtgacg
     5941 ctcatcctca tcatcgccta cgagagatca cacctcctct ccgcgctcgt cttcgccatc
     6001 gccgtgttcg cgttcatcga ggccagcttc aggaggcgac tggccaggtt catagtgagc
     6061 gtcaacgtcg cgctggcggt agtaggcgcc ctcgtactct tcgaacagta cttctggagc
     6121 gtcatcgtct tcgcggtcct cggcatcggg ttctacatcc tgtgggagaa tctgcgggag
     6181 ctcggacggt agcacccgag accggacggg agacccgggg tgggacagcc atacttcagg
     6241 cgtcgagcac gacgtcggag aggggactcc cgatgcagat gaggcgtagg ccctgctcca
     6301 gctcctcgtc gccgatggcg aaagccatgt cctggtcgat ctcaccttcc aggcactttt
     6361 ggatgcacgt ggtgcatgtg ccactgcggc agtcataggg gaggtgaaga ccggcgtgct
     6421 cggcctcttc gaggatgtac tcgtcctccg cgacgtctat ggtgatgccg ctctgcttga
     6481 aggtcacttt gtgagatctg gccacgttcg ctcccgccaa gtcctgtcgt cgaaccgttt
     6541 gttctggctt gttctagcat acccacccgt tcgattcggc gaacgtttgg acggtttgta
     6601 cacccggcaa ccggcggtca cgagcacagc ggcgtcggac agctgacacg ttaccgattt
     6661 taaggcgaga aagcctcccg cttcagcgga ggggatgaat cgcctcgaag cctcgccaac
     6721 ggcacactct tgttattgct tatcacctcc gggatacttc tctaactcat gttgctcaaa
     6781 aaagtctaca ggttcaggat gcgccccacg aaggctcagg aggccgctct gtaccggatg
     6841 gccggagccc gtaggttcgt ctacaactgg gcgcttgagc ggcgcaagag ctactacacc
     6901 gaacacggca agggcatctc cgccagagtg cttcctccga gttgacggcc atcaagggtg
     6961 agccggatac gcactggctt aaagaagccg actcgcagat gctccaacaa gctctcaagg
     7021 atgtaaagcg ggctttcgat gcgttcttcg agaagcgtag ccgctttccc cggtttcgct
     7081 ccaagaaggc cgggcacttc acgtttcgca tcccgcaacg ggtcaaggtc aaagagggca
     7141 aagtctacat tccgaaggtc ggctgggtcc gcatccgcca atcccgcgcc ctcgagggca
     7201 cgacgaagag cgcgacgttc aagagagacg cttgcggaca ctggtacgtt gccctgacca
     7261 ccgagttcga gatccccgtg gcttcgctgg aggcgcccgc tacggccaag ccggtcggcg
     7321 tggacctcgg actcaaggac ctgttcgttc tctccgacgg cggacgggtt ccggctcccc
     7381 ggttcgccag gaaggggagc cgcaaactca gggaagctca gaaagggctg tctcgaaaga
     7441 agccaggaag tcgtcgcaga gagaaagcaa agcagcgcgt ggccaaagtg catcgcaaag
     7501 tgcccaacca aaggagcgat tttctgcaca aactgacgac cggcctgaga cgcaagtacg
     7561 actgcatctg cgtggaggat ttgagcgtga agggacttgc gaaaaccaag ctgtcccgct
     7621 cgatccacga cgccgccttc ggcgagttcg tgcgtcaagt cgaatacaaa gccgcgtgga
     7681 accttcggca tagcgtcaga gtcggacgct tcttcccgtc cacgaagctg tgctctgagt
     7741 gcggagcact aaatccgaac ctgaagcttt cggatcggac gtggctgtgc gtttcttgcg
     7801 gaactcacca cgatagagac ctcaacgctg ccaggaacat actcgccgaa gggctaagac
     7861 tcgtcgccgc cggacagacg gagacggtaa acgctcgcgg agagcctgta agtcttccca
     7921 agggaagcac ggttcgatga accgagaatc ccgccgcttt agcggatggg agtgtcaacg
     7981 gatcagaccg aggttccgcg ccacggtgac ggcttcggcg cggttcttcg cgccgagctt
     8041 gcggtagatg ttgcccgtgt ggctcttgac ggtaccgacg gagacgaaga ggtcgccggc
     8101 cacttcggag ttggttttgc cagaggcgag gagggccaac acctcgagct cacgctcgct
     8161 caagggatct tccaggcggt gcgacgcccc gttgacaccc ggctgctcac gccgcaacgc
     8221 ggtatccact cccggcaact cctggctgcg gtgtccagcc agggcgtagg ccacgatgcc
     8281 gccttccgtc tgagtctcta gatagccttt gcgggccaac ccatccaaca tcttcgacgc
     8341 ctcatctacg gtgagcgagg tccgcatcgc ggccgcggcg ggggtgaggc tgccgccctc
     8401 ctccagggcg ccgagtattt cttgctcttt cctcctcacg ccgaggaagg cgccgcgcga
     8461 cgtttcgtgc ggcgctatga cgcggttgat ctgccccaag agaagcactg gggccacgac
     8521 gccgagtccg aagattgcca accagactat ccccacgtcg gcgagtcccc ggtcgatcac
     8581 gaacgcgacc gatacgatca ggatgaagaa gcaccacacc ccgacgccga gcaccgccag
     8641 aactcggatc aacacggcgc atccccgaga gtcaggccga gtaactccag agcgtgcctg
     8701 tcgcaatcgt cttcggtccg accgggagcc ccgttgcttt gatactccat ccgaggtcta
     8761 ccatcctatt tccgtccgat acccgggcgc tgcctgcttc actcctgtcg gcgacactct
     8821 ccagagtata cgcagatgca cgacccaaag attcaagacc cgcgagcgaa agccccggtc
     8881 ccatgagccg ggaaccgggg cttttcctga ggcctgaagc ctacttctgt tcgaagtcct
     8941 ctccaggagc gccgcagacg gggcactcct ccgggggttg gacgccctgc aactcctcgc
     9001 cgcacgtggt gcagaccatc gtcacggtca tcggcgggtg aggggcctca ccgtcttcgg
     9061 cttctattct ctctcccgcg tcacgggcga ggtcggcgat ggagactttg cccgtaatga
     9121 cgtcctccat cgtggtgcgc gctcctgaga gcgagtcctc tacgagatcg aggtctatgt
     9181 tcgtcgtgcc gcgttcgcgg gcgagcttct ctgtcatgtt gcgggagatg ttgcgcataa
     9241 agcctttggg cgcacgctcc aggcgctcta tggcgtcgtc ggtccaggtg aactctgtgg
     9301 agcctccctc ggggacgctg gcgccggcct tctggaaggc gctgtggtcg atgggacact
     9361 tcgcgcccgt ggtctcctgc acctgctctt tggcgtggct gaccgggcag ccgccggact
     9421 ttacgtcggg ggcttcgcgg cccgtctcct cgcggtactg ctgctcgacg tactcgcgcg
     9481 tgatcgtggt gtagcccttc ttgagcgcgc tcttctcgac gcgcgccttg acgcggcgct
     9541 tctcctgcca ctcttcgagg gagtcgagca gggccgtggc gtcgtcggtc cagtgcaact
     9601 cgcggccgtc gtagacctcg gagaggttca ggttctcgtc ctctgaggtg gttgctatct
     9661 cggcgtccac ggcgtgaaag tgggtcggac ccgatccgca gacaggacac ttcgcgggct
     9721 tgcccttcgc aacgtaacgg cagacgtcgc attcgaagcg gtcctggccc tgggcggagc
     9781 gccggatctc gtcctgaacc tcagcgctga cgccctctat gtcgccctgg tcctcgctct
     9841 tgccgaagcc gccgcgaccg gcatcgaaga tctcctgctt cgccgcagcg tcgctcccgt
     9901 tcgcgccctt gttgtggccg ttggtcccgt tggccttccc ggcggtccgt tcgtcgaagt
     9961 ctttgaagag ggagtctatg ggcttgtctt cgcccatcgc ctcggccgcg cgcatctggt
    10021 ctccgatggc gcgcatcgac tccatggcgc cgggggggag gatgctcttt atgacctcgt
    10081 cgatgacgcc cgtggttatg accgtgtagc ccttctctat ggcgtagcgg atgatggcgc
    10141 ccgtcgccat cccgacgacg aagccgggga tgcggtcgag acgctccagg gcctccttcg
    10201 tccacgtcat gtgctcttcc gcggtgtaga ggtcgggggc ctggtactcg tagttcgtta
    10261 tgaggacgtt cgtcggcagg tagcgcatca tgttctcggt attgccgccg atgtccatct
    10321 cgtcgtcaga gtggtagccg atgcggccca tgacgaccat cgtcgggttt acctcgttta
    10381 cgtacttcag cgtctgctcg aacggcttgc cggcgaggag cgtcgtcttc aggtccacgc
    10441 cctcgtcctc ggcgatcttc ctggctatct ccaggtgggc cgtgtagatc ttggccaacc
    10501 cggagtcgat gatctcctcg tgcagcttct cctgctcctt gaaccggaac accttctggg
    10561 ccttggtgga gagcacgcct gctatggagt ggaacatcgc gtagtggaag tatgggtcga
    10621 agacggagat agcctcgacg gttccgccga actcccctgc aagttctatc gccctcttga
    10681 ggccgccgag agacttggcc gagccgtcca cggcgacgac gatgtgctcg aagggactgc
    10741 ggtcgaggtc cttgacgatc agggtgtccg ccttctggag ccggcgcacg acgcgctcgg
    10801 taacggtacc gagtaccgtg tccttgacgg cggccatgcc catcgcgccc ataacgacga
    10861 ggtcgaaatc gttctcgttg acgtcctcga cgatgacctt gaagttcttg ccttcgagcg
    10921 agcgcctgac gagctcgacg tcgtacttct cgcagagtgg ctcgagcacg tcgatgtagg
    10981 agtcggtgat gatctccagt cccttggtga tgagctggtc gtggatcttg cgctgccgct
    11041 tcatctcgtc ctcgtcgcgg aactcctcgg ggagcccgct ctccatctga cggaagcgga
    11101 catcgtgcat cttggcggcg taggcgtggc aacccgcgac cctgcccccg aactgccgcg
    11161 ccacgtcgac gccgatgacg cacgcctggt tggagtagtc cgaattgtct accgggacgt
    11221 agatctcttt gtacatatac cccgttctcc tagctttgca agacgccgag atttttctag
    11281 atccgtcgtg cgcgaagaac cgttcttctc cgcgccaacg tagcttaccg tttagcccta
    11341 tacaccgcaa ataaaagcgt tcacactgcg ggtgcgacac ggagagagcg ccccggcttt
    11401 cgaggagacc cgctccgcat cgctggacaa ccacgctcgg ctgtcgcaag atggccagac
    11461 gcggagagag cgaggtaaga gatgggcctg gagaagatgg agcaggacgt gagggcgaag
    11521 gatcacgggt cttacgctga gggcgcggcc ttcgtcgaag gacggttcgt gcccatcgcg
    11581 gaggcgcggg ttccgattct ggactgggga ttcttgcgct cggatgcgac ctatgacgtg
    11641 gcccacgtct ggcgcggcag cttcttccgc ctggaggacc acatcgaccg cttcgagcgc
    11701 tcgatggaac atctgcgtct gagtctgccc tacgatcgga atgagatccg ggacatcctc
    11761 atcgagtgcg tgcgcctgag cgggctgcgc gacgcctacg cagagatcat ctgcacccgt
    11821 gggatcccca gaccaggctc gcgcgatccg cgtgactgcg acaatcgctt ctacgccttc
    11881 gttgtcccgt tcatctggat cgccgacccg gagaaacagg agcaaggtct gcacgccgta
    11941 atcgccggta agcagcgcat agcgccagag tcggtggacc cgaccgtcaa gaactatcac
    12001 tggctcgacc tcgagaccgg gctcctcgaa tcctacgagc ggggtgggga gacggtgatc
    12061 ctggtcgacg ccgaggacaa cgtcgtagaa ggacccggtt tcaacgtctt cgccgtaaag
    12121 gacggcacta tctccacccc tgcccgaggt gtcctggaag gtatcacccg caagaccatc
    12181 atcgaactgg ccgccgaaca cggtataccg ttagaggcgc gccccgtacc ggccgacgag
    12241 ctcaggcaag ctgacgaggt cttcctatcg agcaccgccg gcgggatcat cccggtcacg
    12301 atcgtagaca gcgaggccgt ggacaacggg aagccagggc cccttaccag gcaattacgc
    12361 gccgcgtact gggagctcca cgacgaccca cgcttctccc tacccgtccg ctacgattga
    12421 acctggaagt gggaattgtt tgctctacac ttttcctaac ttctggttca cagttcctac
    12481 ttctcagggg taacttcgcc ggcagaaccg tccacggtga tccgccttcc tgtttcgatg
    12541 agctctgtgg catcaggcac gccgacgact gcagggatgc cgtactcgcg ggcgacgata
    12601 gcgccgtggg acatcggccc gcccatctcc atgacgaggc cgcctgcggg gaggaacagg
    12661 ggcgtccaac cggggtcggt agagggtgcg accaggatct cacccggctc caggccggcc
    12721 tcacccgggt ccaggatcac acgcgctttc tgcgtcacca ccccgcccga agccggcgtg
    12781 ccgcgcagga caccatccgc cccggtcgca tggtgtgtcc ctgcggtcga ttcggtaccg
    12841 tcggagagca agacgcgcgg tacgtgtctg cggtctatct cctgctcgta gacggcgcgg
    12901 cggtcgcgga ccagagggtg gagatcctca ccggccaggg ccgcccacgc ctctggcatg
    12961 gagaggaaga agatatcacc ggcgctctgc agtcgaccgg cctcagcaag ctctgcaccg
    13021 gcggtccaca gcagctctct ggcacgagcc atgagcagaa tgatgcagaa cttgggcatc
    13081 tcccgcagtc ctgatagcgc cctggcgcga cccagcagga accggacaag cgccccgcga
    13141 agacggccct tgcgggtggc gcgacgggtc agctcgccga ccatctgttc ggcctcgcgt
    13201 gtggcccgtg cgaactgtac gtcgggggct gcctcagggt cgtccaggcg caggtagttc
    13261 gccagcacgg agaggatgta cgtagggtcc tcagaccacc ggggcaggcc gaggtcgatc
    13321 tccgccaccc cacgatgccc gtacaggcgc aggaaatctg ccagactctt ctggagcttc
    13381 gggggcaggg agccgaggcg atagtcttct gccaggcgct ccggcggagt ttcacccaac
    13441 attcgggcga cgtccgggtc tgcacgcgcc tctttggcga gcgaccagag cgctagatcc
    13501 atctccgtcg tcgggttgtg cgggagcgcg cgcatcgcca cccgcctttc gttgtcggtg
    13561 gcgagtcctc ccagtagctt tccggcgaga gcattggcag cgaggccggc ggcgaagacc
    13621 gggggtacgt tgggcagcag gtccggcggg ccgtcgagca acaggcgctc ggcggctgcg
    13681 aggcactcgg cggcatcgtg gggtacgtcc tccgccgcgc ggaactgccc ctcgacacgc
    13741 gccgcgcggg ccctcgcctt ccccgggcgt gcgagcgcct ctacgacgcg cagtgggatg
    13801 cgtcctcgca cgaaaacggt gaggatggag tcgaggacgg gtcgcatggg tgtcggtact
    13861 ggggcaaggc gaggatctgc cgccaggtgc ctgacgatag cgaccgttcg tgcctccatg
    13921 ttccgcatcg cccactcgaa gagcccgcgt cctcgcttgc tgcgcagcac gggcgttatg
    13981 tccgcgaaga tgcgtccggc ggcctcggtg aagaaggccg cgcctttgta agggttgcgc
    14041 ggcggacggc ctgccattgt ggccaccgcc gaggttatga gtcggaacgt ctggatgccc
    14101 atcggggtca gcggacggta gacgccctgg gcgacgctga aggagaagta cacgcgcagg
    14161 tcctcgtcat tcgtcgaggc tccctcaggc agggggaaga gggtcgtgat cggacgcgcc
    14221 tgcaacagcc agatcttccc gctactatca atggcccact ccgtgtcctg cggttccccg
    14281 tagagctcct cgacccgcgc gcccagtttc gccagagccc ggatctgggt gtcggtgagg
    14341 gaaggctcct cgtccttctc taactcgacg cggcgtgttc cgccgtcctt gtcggcccga
    14401 atcgtcgccc gcttgtcgcc gaggcggcgc tcgaggatct cgcccgtcac cgtgtcgacg
    14461 acgaagtggt cggggttgac cgcgccggag acgaccgcct ccccgagccc gaagctcgcg
    14521 tcgatgacgg cctgccggcg cctacccgtg atcgggttgg cggtgaacag gacacccgcg
    14581 acctcggcct cgaccatctt ctgcacgacg accgcgaggc gcacgctcct cgggtctatc
    14641 ccgttggtgg cgcggtagct gaccgcgcgg tccgtccaca gcgaggccca gcaacaccgc
    14701 accgcctcaa cgagggactc ctccccgacg atgttgaggt acgtgtcctg ctggccggcg
    14761 aagctcgcgc tcggcaggtc ctccgcggtc gccgaggagc ggaccgccac cgcctctccg
    14821 tccccgaacg cccggtacgc agtcgcgatg gtcccggcga cgtcgggtgg cacgggagcc
    14881 gccagcagcg tgtcgcgtgc gttggcggcg agctcggcca ggcgcgcggc gtcgtccggg
    14941 gacgtagttg cgaggtcatc caggatccca cccagcccgg cgcctttggc gacgagttcg
    15001 taggcggccg tggtcacgca gaagccgggg gggaccggca ggcaggcccg ggtcagctcg
    15061 cccaagttcg ccgccttgcc gccggccata ggtagggcgc tgcggtccag gtcctcgaac
    15121 ggcaggacca gcccttctcc cactgtctcc ttctctccgg ttttcatggc acctcctgtt
    15181 gtggccggat agagcaaggc cagaccggcc aggcgacgag aacaatgcgt gcggtggtca
    15241 cggtgtcctc actccttcac gcgcaccggc cagaagccga aggcgacgat gcgggctggg
    15301 atgtcacgca ggattgggac acgcaggaac aggcgcagcg gaagaggagg ggcgaagggc
    15361 tcgttcgagc ggagagcgcg ggccaggacc tgctgctgga tcagagcctg tgccctctgg
    15421 atcatccgcg tcggcaactc gcgccggcgc tgtaccttcg ccaggtgctt cacgtcgaga
    15481 tctctgagct gcgcctggct cgccttcagc ggcctaatga gaacgttcgc cgcgaccacg
    15541 gcgtcctgaa tggcgtagtt gatgcctacg ccagcgaccg ggctcatcac gtgcgcggcg
    15601 tccccgatga gcagcagccc cggcctgtac caccgggggc acatgctcga ttcgaccgag
    15661 agcagggaca cctggctcca gtcctcgatg tactctacgc ggtcggcgaa ctccggtatg
    15721 agctcgccga aaccccggtg tagtgtatcg ataccttcgc ggcgcagccc ggggtaggtg
    15781 cccttgggga tgacatagcc ggcctgccag tagtcgtcgc ggtcgagcat gatggcgatg
    15841 tgcccgcggc cgaagcggcc ttgcaggccc tcggggtccc ccttctctcg cggcagcttg
    15901 aaccagagca cgtccatagg tggcgaggtc ttcatgggct cgaaacccgc cagccgccgc
    15961 acccggctcc cgcgcccgtc cgccccgacc acgaggaccg cgcgcacctc gtgccggccg
    16021 tcctcagact cgtagcggac gccgcggacc acaccgtcct cttcgaccag ctcccgcacc
    16081 cgcgcccgca tcaggagccg gaagcccggg taccgctccg cctcctgcgt gacgaactcg
    16141 aggaagctcg tctggggcat gatcgtgatg tacgggtact tggtcttcag gcggccgaag
    16201 tcgatcggcg tgaatgggcc cgaggcggtc tgcagcgtga agttgcgcac ctcggagtgg
    16261 cgcagctcga gcaaa
//
LOCUS       DATMVF010000081         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_286483, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000081 DATMVF010000000
VERSION     DATMVF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286483"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..1009
                     /locus_tag="VN178_03580"
     CDS             530..1009
                     /locus_tag="VN178_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80081.1"
                     /translation="MVYLDTGPLPDGTALIDTFPPEARRHTERQVEELGDGWRLPMPP
                     WDELAQQASLEGLGDDQLRLLRSKAVAQPFGTYTQELRPENPAREELPKLGILCSFSV
                     DLVQEMIASDNPLTRGFVGPGWRFVDLPTGHWPMFSRPDDLAELLLGLLPLGFAEAV"
     gene            1042..1929
                     /locus_tag="VN178_03585"
     CDS             1042..1929
                     /locus_tag="VN178_03585"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWS80082.1"
                     /translation="MRYAVLGVGGVGGLVGGALARAGHDVSLLVRPGRREHYSEHLSV
                     QSELLGNFEAPVRVADWLDEQCDVVWITVKAPALEAALGTIPPEVLGDALLVPLLNGV
                     DHVGRLRDGYGAERVLPGTIRVEAEQAGPGRVRHHSAFADVQVAPNPATQERTQTLCE
                     ELRDAGMGCEVQDDETTMLWSKLCFLAPFALATTASEGPLGDVREDPGRWVSLEQCVS
                     EACAVGAAEGAMVAPEPILTALEGLPNGFRSSMQKDVAAGKVPELDAIAGPILRGGSE
                     HGIEVSATRSLVERVLALE"
     gene            2068..2397
                     /locus_tag="VN178_03590"
     CDS             2068..2397
                     /locus_tag="VN178_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80083.1"
                     /translation="MTNDALIEEINAAYRRLGAATEDLASADRELAEYVRRVRLDNAE
                     AILEARNERTASLYLDGLLDTHDHRRLEADRARADLALLHARREVERLQLIVRLLGTR
                     PSEGIPG"
     gene            2431..2904
                     /locus_tag="VN178_03595"
     CDS             2431..2904
                     /locus_tag="VN178_03595"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HWS80084.1"
                     /translation="MPEASAYTDGASTGSRGPGGYGVVIRWEGKIKEISGGEPDTTNL
                     RMELTAACVALESIEEGYTVSVYSDASYLINCMRRGWYKKWRENGWLNYRNEAVANKD
                     LWERLLGAARRHQRVRWKKVKGHQKTQGPHKSGNDRADELAVAAKNEAARPSLPS"
     gene            complement(2934..>3348)
                     /locus_tag="VN178_03600"
     CDS             complement(2934..>3348)
                     /locus_tag="VN178_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWS80085.1"
                     /translation="YLEEERIEAELHRIGADSTRYWNPINTTTPHDILHVLKKIADPS
                     YTSPRLSADMLELMTNTSFEDRLPQPLPEETRVAHKIGSYESTFSDAGIVFPGKSSGT
                     GREYFIVVFSEGTSEGEARGAIQDISLETYRAFFR"
BASE COUNT          635 a          992 c         1147 g          574 t
ORIGIN      
        1 caccgaaagg ctcgaagccc gcgagcgcga gaccaccggc ccgaacgggc agaggaccac
       61 cgtcaccgac aacgtcattg ccctaagacg ccttgcgcgg tgaccagccc gccgaaggcc
      121 gcctcggatc acccaccggc cggcgtttct catgcgaacc cgattcttcc tatcggtagc
      181 cgggacttgt gacgaagtag gctttcgtcc ttggccctct caggagaggg gcgcaccgac
      241 caacgcggcc aggtacatgt acacccacgg cttccaacca cttcgctcct gctgcgttgc
      301 ttgcgcgttg gagctcgcct cgcaaaaaac gtcgaaaaag ggactcatac ccccgaaatt
      361 catcgggaat tcatcggatg ttgatactaa tacgggactg cacccgcatc tgtgtgggct
      421 gtgagaaggg cagactcatc gcgaggcgca gggcgtagag gagcgtttcg cttgcggagt
      481 ggtgcgcagg acgtaaggac aggatcggat ccccgagcga atcttccagt tggtatacct
      541 ggacaccgga ccgctccccg acggtacagc cctcatcgat acgttccctc cggaagccag
      601 gcggcacacc gagcgtcagg tagaggaact cggcgacggt tggcggttgc ccatgccgcc
      661 ttgggacgaa ctggcacagc aggccagcct ggaagggctg ggcgacgacc agctaaggct
      721 actgcgctcg aaggccgttg cgcagccgtt cggtacctat acccaggaac tgcgtccgga
      781 gaaccctgcc cgagaagagc tgcccaagct ggggattctg tgtagtttct ctgtggatct
      841 ggtacaagag atgatcgcca gcgacaaccc tttgactcgg ggcttcgttg gccccggctg
      901 gcggttcgtg gacctaccca cgggacactg gcccatgttc tcaaggcccg atgacctagc
      961 cgagttgctc ctcggcctcc tccctctggg cttcgcggag gctgtttaga atagagtaca
     1021 aacagttgat ggaggtgggt aatgcgctac gcggttctcg gggtgggtgg ggtcggcgga
     1081 cttgtgggcg gtgcgctcgc gagggcgggc cacgacgtta gcttgctcgt gcgtcctggc
     1141 cggagggagc attactccga gcatcttagc gttcagagcg agctgctcgg aaacttcgaa
     1201 gctccggtac gggtcgccga ttggctcgac gagcaatgtg acgtcgtctg gatcacggtc
     1261 aaggcgccgg cgctcgaggc ggcgcttggc accattcctc ctgaagtact gggagatgcc
     1321 ctcctggtgc cgctgctaaa cggcgtggat catgttgggc ggctgcgcga tgggtacggt
     1381 gccgagcggg tcttgccggg gacgataagg gtcgaagccg agcaagcggg tccggggcga
     1441 gttaggcacc actctgcgtt tgccgatgtg caggtcgcac cgaatccggc aacgcaggag
     1501 cggacccaga ccttgtgcga agaactgcgt gacgccggga tgggctgcga agtgcaagac
     1561 gacgagacca ccatgctctg gagcaagctc tgctttctgg cgccgtttgc gctggcgacc
     1621 acggcgtcgg agggcccgct cggggatgtc cgggaggacc cgggccggtg ggtaagcctg
     1681 gagcagtgcg ttagtgaggc ctgtgccgtc ggtgcggccg agggggccat ggtcgccccc
     1741 gagccgatcc tcacggcact agaagggctg cccaacggtt tccgaagttc gatgcaaaag
     1801 gatgttgcgg cggggaaggt gccggagctc gatgccatcg ctggacccat ccttcgtggc
     1861 ggtagcgagc acggcatcga ggtgtcggcg acccgttccc tcgtggagcg ggttctggcc
     1921 ctagaatgac ggcgaatgaa cactcaggcg ggcccctgcc ttccgcaccc agctccgccc
     1981 tccgtcgctc ctgggtagga caggggcatc ggcacgagag gttcggatcg gcatcgagga
     2041 taccgcagtc gaggagagga gtctccggtg actaacgacg cgctcatcga ggagatcaat
     2101 gcggcatacc gccggctcgg cgccgccacg gaggatctgg ctagcgccga ccgcgagctg
     2161 gccgagtacg tcaggagagt acgcctggac aacgccgagg cgattctgga ggccaggaac
     2221 gagaggacgg cgagcctcta cctcgacggc ctgctcgata cccacgacca tcggcgcctg
     2281 gaggcagacc gggcgagggc cgatctcgcc ctcctgcatg ccaggcgcga ggtcgagcgg
     2341 ctgcaactga tagtccggct gctggggacc cgaccgagcg aggggatacc aggatgagtg
     2401 gtagggggat gcgcgagagg ttcggctccg ttgcctgagg cctccgccta caccgacggg
     2461 gcttccaccg gctcccgtgg tcccgggggc tatggggtcg tcatccgctg ggagggcaag
     2521 atcaaagaga taagcggcgg cgagccggac accaccaacc tgaggatgga actgaccgcc
     2581 gcgtgcgtcg ccctggagtc catagaagag ggatatacgg tgagcgtcta ctcggacgcg
     2641 tcctacctca tcaactgcat gaggaggggc tggtacaaga agtggcgaga gaacggttgg
     2701 ctcaactacc gcaacgaggc cgtggcgaac aaggacctgt gggagaggct cctgggagcg
     2761 gcacggcgcc accagcgagt gcgctggaag aaggtcaagg ggcaccaaaa gacccaaggg
     2821 ccacacaagt ctggcaacga ccgggccgac gaactcgccg tggccgccaa gaacgaggca
     2881 gccagaccat cgctaccgtc gtgaccatga ggagatggca cgcgcaaact gctctagcga
     2941 aagaaggccc ggtatgtctc cagggagatg tcctgaatcg cccctctggc ctccccttcc
     3001 gaggtgcctt cggagaagac gacgatgaag tattcccgac cggtgccgga gctcttcccg
     3061 ggaaatacaa tcccggcgtc tgagaaggtg ctctcgtagg atccgatctt gtgcgccacg
     3121 cgggtctcct cgggtagcgg ctggggcagg cgatcctcga aggatgtgtt cgtcattagc
     3181 tcgagcatgt cggccgagag ccgtgggctg gtgtaggagg gatcggcgat cttcttcagc
     3241 acgtgcaaga tatcgtgcgg ggtcgtggtg ttgatgggat tccagtagcg cgtggagtcc
     3301 gcgcctatgc ggtggagctc ggcctctatc ctctcctcct ccagatag
//
LOCUS       DATMVF010000082         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_286775, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000082 DATMVF010000000
VERSION     DATMVF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286775"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VN178_03605"
     CDS             complement(<1..446)
                     /locus_tag="VN178_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWS80086.1"
                     /translation="MITTTAEILIVEDDEVIMGTLAYNLSRNGFGVSQATSGADALRL
                     ARKLRPDLILLDLMLPGESGIEVCERIRERDQSVMIVMITAKDAEEDKVRGFEAGADD
                     YVTKPFGMKELIARINANLKRSVTGGRGKIIEAGDLHLDTKNFTAQA"
     gene            complement(511..1167)
                     /locus_tag="VN178_03610"
     CDS             complement(511..1167)
                     /locus_tag="VN178_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS80087.1"
                     /translation="MSAEERALISRASRGDVDAFSRLVSAHSALVRGVTLRMLGAQES
                     QDASQEVWVRVWANMKSFRGESAFSTWLYRVTVNTCLSFRRKESRRRTREVEEELAHL
                     SLSSGGDGDPEEAALDAERREEIQVALGSIRAEHRAAMVMRHMEGLSCAEISEALGVP
                     DGTVKGWASRGRTAMLAALTQNSASADRIIRPTKQAGRRATTPEPPVPDASVKARLKE
                     "
     gene            complement(1282..1521)
                     /locus_tag="VN178_03615"
     CDS             complement(1282..1521)
                     /locus_tag="VN178_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80088.1"
                     /translation="MALDLSMFNGSVSTLLNHLTRHYGEDAKLETYVICARVRSAAIP
                     GGSGINWIVNPGGEELAGGLLREVLKAVEGNGDLS"
     gene            1678..>2102
                     /locus_tag="VN178_03620"
     CDS             1678..>2102
                     /locus_tag="VN178_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80089.1"
                     /translation="METSVKTVRVHTERTFAIARSSADTFERVIFEMSEVGVTGRGEA
                     APTSYYRQDVRAVATALETVEVWDPWDIEGTLAHNGAMPPSALTALDNAMHDLAARRL
                     GVPVYRLLGLARPVPVSAYTLGIADKETTVAHAQRLKKHR"
BASE COUNT          367 a          716 c          620 g          399 t
ORIGIN      
        1 gcttgggccg tgaagttctt cgtgtccaga tgcaggtccc cggcctcgat tatcttgccc
       61 cggccgcccg tcacgctgcg tttcaggttc gcgtttatgc gggcgatcag ctccttcatg
      121 ccgaaaggct tcgtcacgta gtcgtcggcg ccggcctcga acccgcggac cttgtcctct
      181 tccgcgtcct tggccgtgat catcacgatc atcacgctct ggtcgcgctc ccggatcctc
      241 tcgcacacct ctatccccga ctcgcccgga agcatgaggt cgagcaggat caggtctggc
      301 ctcaacttcc tcgcgagcct caaggcatcg gcgccgctgg tcgcctggct gaccccgaag
      361 ccatttcggg agaggttgta agcgagggtg cccatgatca cctcgtcgtc ctcgactatc
      421 aggatctccg ctgttgtggt tatcaaggca tctactcctg ttggatccgg ccaacggcta
      481 cggccagtat agatgctgct ctcacatgct tcattctttt aaccgagctt taacggaggc
      541 gtccggcacc gggggctccg gcgtcgtcgc tcgccttcct gcctgtttgg tggggcggat
      601 gattcgatcc gccgatgcgg agttctgggt cagtgcggcc agcatggcgg tccttcccct
      661 gctggcccat cctttgaccg taccgtcggg gacgccgagc gcctcggaga tctccgcgca
      721 ggagagaccc tccatgtgac gcatgaccat ggccgcgcgg tgctcggccc ttatgctgcc
      781 aagggctacc tggatctctt cccggcgttc cgcgtcgagc gcggcctctt cgggatctcc
      841 gtcgccgccc gatgataagg agagatgggc cagttcctcc tctacctcgc gcgtcctgcg
      901 gcgggactct ttgcgccgga agctcaggca ggtgttgacc gtgaccctgt agagccaggt
      961 cgaaaaggcg ctctcccccc tgaagctctt catgttcgcc cagaccctga cccagacctc
     1021 ctggctcgcg tcctgggact cctgggcccc cagcatgcga agcgtcaccc cgcgcacgag
     1081 ggccgagtgg gcgctgacca gcctcgagaa cgcgtcgacg tcgccgcggg aggcgcggga
     1141 gatcagcgcc cgctcttcgg cgctcaaagg cgatttccct ctgtcgaagc acagcgaaga
     1201 cagccggaag gcaaaaccgg agcctcgcac ggctcttggg gagctatagg atcgaccatt
     1261 actgccggca gcaagaaacg ttcacgacag gtctccattg ccctccacgg ccttgagtac
     1321 ctcccgcagc agcccgccgg cgagttcctc gccaccagga ttcactatcc agttgatccc
     1381 gcttcctccc ggtatcgccg cgctccgcac cctcgcgcag attacgtagg tctcgagctt
     1441 cgcatcctcg ccatagtggc gggtgagatg gttcagcagc gtgctcaccg aaccgttgaa
     1501 catggacagg tccagagcca tgcgtacccc cgtttttctg tcttggccat cctgatccca
     1561 atattacccc gataaccatc cgcactacca ccaggaagtc gagatactgc tcgagaaaga
     1621 aggacacgcc cgccaccgct acaatgcaat ctcgaagaga ctggaggagg ggcggaattg
     1681 gagacgagcg taaagacggt cagggtgcac accgagcgta ccttcgcgat cgcccgcagt
     1741 tcggcagaca ccttcgagcg tgtaatcttc gagatgtcgg aagtcggcgt cacgggcagg
     1801 ggcgaagccg cgcccacgag ctattaccgc caggacgtgc gagcggtggc gacggctcta
     1861 gagaccgtcg aagtctggga cccctgggac atcgagggca cactcgcaca caacggcgcg
     1921 atgccccctt ccgcgctcac ggccctcgac aacgccatgc acgacctcgc ggccaggaga
     1981 ctcggcgtgc cggtctaccg tttgctcggg ctcgccagac ccgtgcccgt ctcggcgtac
     2041 accctgggca tagcagacaa ggagacgacc gtcgcccacg cgcagaggct gaagaagcac
     2101 cg
//
LOCUS       DATMVF010000083        24204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_151642, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000083 DATMVF010000000
VERSION     DATMVF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 24204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24204
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151642"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VN178_03625"
     CDS             <1..466
                     /locus_tag="VN178_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HWS80090.1"
                     /translation="KITGVGRTTDAYNLTDPDPKGFGIMRAMQLAVADAGLEGEQIGY
                     INPHGTGTVAGDGPESQAMARINPNVKVSATKSTLGHSLGASGAVETAICALAIKERK
                     IPPMRNLTKVAEGCADLDYVVDEPREAPDLEAALCANLGVGGHNAAVVLERA"
     gene            466..639
                     /locus_tag="VN178_03630"
     CDS             466..639
                     /locus_tag="VN178_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80091.1"
                     /translation="MSSMDLPENVYEVPQGRPEPADDGACHHLAGMRIASLSLTSTAE
                     DPVVRRLSERTAG"
     gene            653..1507
                     /gene="larE"
                     /locus_tag="VN178_03635"
     CDS             653..1507
                     /gene="larE"
                     /locus_tag="VN178_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWS80092.1"
                     /translation="MNPVTTDVLHNRLTELEQILAPRGSALVAFSGGVDSSLALAVAA
                     RALPRHRVLAVTSNNETYLPSELDLAREFAESLGVEHLVVNTRELDNPNYASNPTNRC
                     YFCKSTLYSDLATLAEQRGYACVVDGANKDDEGDYRPGRKAARELGVVSVLSEAGMTK
                     AEVRELAKHLDLPTWDKPALACLSSRFPYGQEITPEKLSQVARAEEFMRREGFSQVRV
                     RHHGEIARLEVGPEELERAFAMREEISAELLDAGFLYVTLDLAGYKSGSLNAALKKGK
                     KALPVISS"
     gene            1511..2059
                     /locus_tag="VN178_03640"
     CDS             1511..2059
                     /locus_tag="VN178_03640"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HWS80093.1"
                     /translation="MDGVEGPLLALTLVLMFVGLLGSVLPGLPGVTLIFLSALVYALL
                     TDFRTIGAAVLVALFIFAALALVIDFVATSYGARRFGASNWGTLGGALGGIAGALIGL
                     LFLGIGSLFGLILGTIAGVFLGEYLKRTRQDQQEPGSTRSDWRRASHAAGGVLVGYLA
                     SAVIQGLLGLASIVAFVLGVLY"
     gene            complement(2231..3115)
                     /locus_tag="VN178_03645"
     CDS             complement(2231..3115)
                     /locus_tag="VN178_03645"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWS80094.1"
                     /translation="MDIRHIDIDWTPELERPVLIAGFTGWNDAADAASVAVGALASSW
                     EARRFGGFDGEEFFDFQTTRPQVKLVDGVTREIQWPENMLSATEPRLEAAGGRGAVLL
                     TGPEPNFRWRAFSAAVVELAKALNVELVVTMGALLADVPHSRPVSVSANSQDASLVDS
                     LGLSASRYEGPTGITGVLHRACADAGLPSVSFWASVPHYLPAVPSAPAALSLLEKLSG
                     LLGMEVDTADLESTAETYQEQVSVAVSQDSDLSSYVRMLEDRFDSQADQGPRNLPSGD
                     ELAQELEGFLREHRREED"
     gene            complement(3163..3735)
                     /gene="aat"
                     /locus_tag="VN178_03650"
     CDS             complement(3163..3735)
                     /gene="aat"
                     /locus_tag="VN178_03650"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWS80095.1"
                     /translation="MKLTTEILEACYRAGAFPMADAYGRIEFYRSDPRSVLEFEDLHV
                     PHSLERVIRKGTFELRVDEDFDGVIRACADREETWISPEIIDAYVGLHHAGKAHSVEA
                     YRRGELAGGLYGVTLGGAFMGESMFTRIRDASKVCLVYLVNRLQERGYALLDCQIQNP
                     HLARLGAREIPEQEYLRRLQHALTLECSFA"
     gene            complement(3807..4814)
                     /locus_tag="VN178_03655"
     CDS             complement(3807..4814)
                     /locus_tag="VN178_03655"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HWS80096.1"
                     /translation="MNSQVPGEAALPEDAPLAAVTRGALLESVHRGRLVICDPDGKVL
                     EAAGDAEAYIYARSSAKPFQALPLVRSGAADAFGLTDNELAVVCASHNAEEPHLTAVT
                     SILEKAGLTEDDLQSGAHLPLYEPEADRLIRSSEEPRPIHGNCSGKHAGMLAVCVHEG
                     YDTATYRDPAHPLQRRILGLIAEVCGLRDDEVLVAGDNCGVPAFALPLRSFATGLARI
                     ATGKALSDELALAALRIGDAMREHPFMVAGTGRFDTELMDSTDLLVKGGAEAMLAVGS
                     QEGWGMALKISDGAVRAVRPAALAVLGGMGVEVPEAVSSVRALHGEKVGEIRPLIQAP
                     G"
     gene            complement(4807..5121)
                     /locus_tag="VN178_03660"
     CDS             complement(4807..5121)
                     /locus_tag="VN178_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80097.1"
                     /translation="MRAGERAGAALRTAHLLRIDSYMDIAILAMWTASPRVDAMLGMA
                     EASLRGGSPGGKDDELIEKLRALVREGREYLAGGDFSVAMGRIRVAHDLLSLHIIRLS
                     DE"
     gene            complement(5118..5933)
                     /locus_tag="VN178_03665"
     CDS             complement(5118..5933)
                     /locus_tag="VN178_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWS80098.1"
                     /translation="MEHDIEALTGGAMASIWSWVRAKIPAGVEVFDAHAHIGTDVDGR
                     TMTGDGMRERMEAAGVKKSIVFPLNDPNARDDFSGPNDVVWRAYEEYPSTFVPFFRLN
                     PHLDYDREFSRCVERGFMGLKLHPVSQEFELDDPRVVRLFGMAAETDLPILIHTGFAM
                     QRIVEPLLPTVEAHPGLRLILGHCAMVEVLSATRAFEGHPNVLFETSVVQAKDLYELC
                     CSVDPSRICFGSDIPYGDLPSTLHSILAAAEAAGVPEETLPRILSENIRRWFP"
     gene            6076..6561
                     /locus_tag="VN178_03670"
     CDS             6076..6561
                     /locus_tag="VN178_03670"
                     /inference="COORDINATES: protein motif:HMM:NF042842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HWS80099.1"
                     /translation="MIAWVLHILELIAGVFGISIGFSRLPNAASALQIVTLTAVGLVG
                     LSAFVRHFIFHKSDARRLGWDSTRPEFQYEVGFANLGFALVAFFVYFGEWGLAPHVAV
                     VLGYGLYLLQAALLHTWKSISGDGGVRRFLQSALPALIFSSVMIYFAVKGMIAANLSV
                     L"
     gene            6642..6830
                     /locus_tag="VN178_03675"
     CDS             6642..6830
                     /locus_tag="VN178_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80100.1"
                     /translation="MEIPKAMIVERIRSQQGAEKAKEADSELPDKVDTEANSDLLQKY
                     GLDPGQLTDIAGGDPAVG"
     gene            complement(6917..7942)
                     /locus_tag="VN178_03680"
     CDS             complement(6917..7942)
                     /locus_tag="VN178_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWS80101.1"
                     /translation="MTTVGIVANPASGRDIRRLVAGASVFDNAEKGNMVFRLMVGLAA
                     VGVERVLMMPAASGLFDGLDRNRRANAASERKQDLPELELVEMKIRHGARDTAYAVGE
                     MCERGVSAIVVLGGDGTNRVVARHCYPHIPLCTLSTGTNNTFPKMQEATVAGLATGLV
                     ATGRVGDDVLRREKILYVSIDGDPDRDCALVEVALNSERFVGARALWRAPDISEVVVA
                     SASPEAVGLSSIAGLLDPVDRSTPHGLYVRLAPPVEADTVLTVPLAPGLIAPVGVVES
                     RRVEPGESVELEPKVGSLALDGEREIEIGPHNEVKVRLEPNGPLTINVGEVMHEAARR
                     GLLDGAP"
     gene            complement(7955..9085)
                     /locus_tag="VN178_03685"
     CDS             complement(7955..9085)
                     /locus_tag="VN178_03685"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin dehydrogenase
                     dihydrolipoyllysine-residue acetyltransferase subunit"
                     /protein_id="HWS80102.1"
                     /translation="MSETQQISKLGMPKWGLSMTEGTVVEWLIEEGTEISKGDEVVEV
                     ESEKINNAIESPAEGVLRRRVAREGEVLPVGGLLGVIAPSEVPDEEIDSFVEEFLESF
                     DPEEAAAEAGGPEPQTVEIGGRNVQYLKIGEGEGPPLILLHGFGGDINIWVFNQEALS
                     EAGRTVYALDLPGHGQSSKDVGEGDLDSFVEILAGFMDEVGVEKAHLVGHSMGGAVAG
                     SFALAHHDKVASLVLIASAGLGEEINEDYIEGFIAANRRREMKRVLGLLFADPDLVTR
                     RLVNDELRFKRMDGVDEALRAVADKMFPDGSQAEGPDLSGLETPVLVIWGQDDQIIPA
                     SHTENLSDGVRIEILEDTGHMPQMESAGRTNRLIGGFLDGLE"
     gene            complement(9082..10101)
                     /locus_tag="VN178_03690"
     CDS             complement(9082..10101)
                     /locus_tag="VN178_03690"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWS80103.1"
                     /translation="MARTISYMQAINEALAQEMERDETVVVFGEDNVGGAGSPGEMDA
                     WGGVMGVTKGLYAKYPERILDTPISESAFIGAAAGAACSGLRPVAELMFVDFFGVCMD
                     QIYNQAAKFRYMFGGKAKTPMVIRTMFGAGIRAASQHSQSLYPIFTHIPGLKVVIPSN
                     PYDAKGLLIASIRDDDPVMFFENKVLYAVEGEVPEEPYTIPLGEAEYVREGEDVTIVA
                     IGRMVSMAEQAAASLADEGIECEIVDPRTTSPLDEETIFESVENTGRLVVVDESNPRC
                     SMATDIAARVAENCFEDLKAAPKMVTAPHTPPPFSPSLEDLYVPDPDRIAEAAREVTG
                     AGARA"
     gene            complement(10206..11195)
                     /locus_tag="VN178_03695"
     CDS             complement(10206..11195)
                     /locus_tag="VN178_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWS80104.1"
                     /translation="MTEVAGARELGKEQLLEAYRIMKTIREFEDRLHTEFATGEIPGF
                     VHLYAGEEAIAAGFMAHLDENDYVASTHRGHGHSIAKGCDVKGMMYEIYGKDEGICRG
                     KGGSMHIADLDKGMLGANGIVGGGPPLVCGVGLSAKIRGTRQVGVSFTGDGGANQGTF
                     LESLNLASAWDLPCVFVIENNGYAEATAHAFHSKGIDIAKRADGFSMPGVTVDGHDFF
                     AVYEAAGEAIRRAREGGGPSLIECKVNRYHGHFEGDAQTYRGENEVEEIRNEKDCLTL
                     FRQRVTSAGLVDEDALDRIDEEVEQLIDEAVDEAKAAADPSQDNLLTDVYVSY"
     gene            complement(11428..12702)
                     /locus_tag="VN178_03700"
     CDS             complement(11428..12702)
                     /locus_tag="VN178_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWS80105.1"
                     /translation="MALVLVGLNGFFVAAEFALVRVRESRILQLEQEGSTRAALVRET
                     LRDLDAYLSVCQVGITVASLALGWVGEPAVSHLIRPVLEAVGITNEGVVSIIAIILGF
                     SAITYAHLVFGEQAPKYFSIQKAERVSLWISRPLNLFKILFNPVVWVVNASTNFVLRP
                     WGIRLGEEMEAHSEEELRIMISSSTASGVLEPEERDYLNKVFDFGDRVAREIMVPRPD
                     IEAIASDAPLPELVDAAVYGRYTRYPVYEEDLDHILGAVHVKDLFRAARTNDEDFDIR
                     SVIRECLVVPENKRIEEILKEFQTRTLQMAIVIDEWGSVEGLITIEDIVEEIVGEIQD
                     EFDEGEPVIEKISDHLYAVDGRIPIIDVNERFEIDLPHEDFDTIGGYILGSLGRPPET
                     GDTVEADGVTMQVKSVDGPRVSMLTLQRNDPS"
     gene            complement(12925..13434)
                     /locus_tag="VN178_03705"
     CDS             complement(12925..13434)
                     /locus_tag="VN178_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWS80106.1"
                     /translation="METNRGSEGILSEEMSGKREEIVELLKTAYFMELETVMNYVTNS
                     INPDGVRAQEVKESIEEDIQEELTHAQQFAARIKELYGVVPGSMEFKASQDTLQPPDD
                     QVDIVHVIKGVIDAEEGAIQHYTRMVEETEEVDPVTQDMVIEILHDEQGHKRLFEGFL
                     REYEAEGLA"
     gene            complement(13575..13964)
                     /gene="dhaM"
                     /locus_tag="VN178_03710"
     CDS             complement(13575..13964)
                     /gene="dhaM"
                     /locus_tag="VN178_03710"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HWS80107.1"
                     /translation="MVGIVLVSHSKEIADGTADLVLQMAGEVEISAVGGDADGSFGTD
                     PEGIEASIRGLETDEVLVFMDLGSAVLSAETVLEMLPPQEREKVTLVDAPFVEGAFAA
                     GVTASTGADAEECVEAAMEARTEPKLQ"
     gene            complement(13964..14596)
                     /gene="dhaL"
                     /locus_tag="VN178_03715"
     CDS             complement(13964..14596)
                     /gene="dhaL"
                     /locus_tag="VN178_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HWS80108.1"
                     /translation="MDATQSASDVVKGMAAAMEENRRYLTKLDSEIGDGDHGNNMNRG
                     FKAALERLEAANPSTPADVLKAVSMALISKVGGAAGPLYGTAFLRASSALEDKEEVLA
                     DDAAEALEAALGGIKQRGKAEVGDKTIVDALEPAVEAAKEAAGEGSVAGVFRSAADAA
                     KEGAESTIPLTAKRGRASYLGARAAGHQDPGATSTYMLLDAAARVLEGSS"
     gene            complement(14600..16117)
                     /locus_tag="VN178_03720"
     CDS             complement(14600..16117)
                     /locus_tag="VN178_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWS80109.1"
                     /translation="MGASEKGPASIAEAFVRSAERSPDKLCLRFEGEEISYQGMRERA
                     EGFGAGLSAWGLRPGDRVALYLGNHPDLLAAYLGTHLAGGVIVPVNSQYRRGELRHIF
                     GDAGVRLCLTDEERKPELERTRLDLSELEAVIETGQGFEDFLGETHAPQMPRGEDLAV
                     IAYTSGTTGRSKGAMLLHRNLVANAQAVCEAWRWTGEDTLFLALPLFHTHGLMVGAHG
                     TFLMEASAELHRKFDAAAAYDALLAGRVTMFFGVPTMYTRLLREAEGRDERPDPIRLY
                     VSGSAPLSPQAFDEFEGLFGERILERYGMTETIMNLTNPYEGERRPGTVGRPFPGQEA
                     RVVGVETREPLDDGEIGEIEVRGPNVFPGYWNRPDATEESFDGEGWFATGDLGYVGED
                     GYFTISGRAKELIISGGYNIYPREVEEVIEGCPGVSEVAVVGMPDPEYGERVVAVVVP
                     EDPGLTAEKVTSFCREDLASYKKPREVVIVEDLPRNALGKVLKHKVSEGLGKPEV"
     gene            16538..17893
                     /locus_tag="VN178_03725"
     CDS             16538..17893
                     /locus_tag="VN178_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS80110.1"
                     /translation="MWKQYSGTTLNFVSENTAPTTAIAANLNGFKELTGINVNVLQLE
                     LGALVQKVALDIGSGEGAYQIVYADPYQIMAPYHAALADLNEFDGDDDLPSIPKGTDD
                     FIPTQLAAAGRFENEQNLYALPYDCPTMIWMYRKDLFDKYGDRMSQDLGFDPQPSPSL
                     TWEQYYQIADWFNENQDEVPYGTGHQARQYDSLMCDFSNILWSYGGDYFHSKEIGSLG
                     TTDPGPCTLDEPNALEAAEFYKKLLSIAHPGSVSWDWNATDEAFRTEQVAMVPNWHEF
                     AAVDQDPAESTIVGKIGYSPLPKGPVRNANMWGGTGVGINGASPINEQKAAWLFLVWA
                     TSPDTMLMGLKSDVGGGTPTRGSLYELPEVKKAMEPPTNLPNLLTYDAVSEAWRPENI
                     GLRPKIQSWNECDTAIYTEVSKMLAGQKTPEQAMKTAKRYFDEAQARVENLASGPTGV
                     A"
     gene            17893..18813
                     /locus_tag="VN178_03730"
     CDS             17893..18813
                     /locus_tag="VN178_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS80111.1"
                     /translation="MAATQRRSSGATKGANGGGRISLEMKMLVPVLALLAVLSLFPFF
                     YIIWMSLNIVSLINGISFDFAGLANWTRMFTDEIVGAAWARAALYFVATVGLEMILGI
                     AIALLVHELVWGQNLVLSLILLPMFMAPVIVGLLGRFLTDSSYGLYAYVLRELGIFES
                     DILGTASSALPAVILMDVWEWTPLIVLIVLAGLASVNQYVIEAAKVDGAGYLERLRYI
                     VAPSIAGVVIVALLVRSMDAIRYFDIITVTTNGGPGNTTKIIPIRLYETAFRFFDLGY
                     AAAIGLGMLVFSILVANVFVRILESRGLAR"
     gene            18810..19652
                     /locus_tag="VN178_03735"
     CDS             18810..19652
                     /locus_tag="VN178_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS80112.1"
                     /translation="MTTSTTGRNSGKQVILYAILFLVLLWTLVPIVWMVLASFKPGVA
                     QLSSTPVFVFKPTMQHYVALFTGGNLGAYFLNSVLAAGFSTVIAVSLGCLAGYGLARS
                     HFRGKQHVAFWIISTRMAPIAAIVLPLFILFRYVNLLDTIPGLIVAYLTFNLPFAIWI
                     MNAFFMDLPPSLEEAAMVDGATRFQAFYRVALPLVTPGIVTTAILCLVFSWNDYAFAA
                     TFAGPDSQTLPIAAAQLNTQTGLNWGQLSAIGTIVVLPMMVVGLAVRRYLVKGLTLGA
                     VTGE"
     gene            complement(19691..20458)
                     /locus_tag="VN178_03740"
     CDS             complement(19691..20458)
                     /locus_tag="VN178_03740"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWS80113.1"
                     /translation="MAVLDAFDLTGKTAVVTGGNTGLGQAFAKALVEAGAGVAVAART
                     RERSEAVAEEIHASGGNAIAVDLDVTDPAQVDGMLEAVTERLGPVDILVNNAGLCYHR
                     PAAEVPLEEWHAVFDVNVHGLWHCAQVVGRRMIQNGGGTIINIGSISAMIVNRPQWQP
                     AYNASKAAVHQLTRSLAAEWAPYNVRVNALAPGYVKTAMSPVDEPQFRRHWIEDAPMQ
                     RYATPEELGPSIVYLASDASSFMTGSILVVDGGYTLF"
     gene            complement(20538..21272)
                     /locus_tag="VN178_03745"
     CDS             complement(20538..21272)
                     /locus_tag="VN178_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HWS80114.1"
                     /translation="MQAYIGEFIGTMILIILGDGVVAGVLLRNSKAENSGWIVITFGW
                     GMAVAIAVYCVGQFSGAHINPAVTIAFAATGGLPLADVIPYIIAQFLGAFVGAVIVWL
                     AYLPHWPETEDEGLKLGVFCTAPAIYNTPANVITEIIGTFVLVFGVSGIVANAGAVSG
                     QAATVIGSGINPLLVGLLVLGIGLSLGGPTGYAINPARDLGPRIAHAVLPIAGKGGND
                     WGYAWIPVVAPIIGGILGAVTWVILF"
     gene            complement(21286..22287)
                     /gene="dhaK"
                     /locus_tag="VN178_03750"
     CDS             complement(21286..22287)
                     /gene="dhaK"
                     /locus_tag="VN178_03750"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HWS80115.1"
                     /translation="MKKVINAPERVVEDMLRGMELAHGGLIRVIPEANVVVRADAPIE
                     GKVALVSGGGSGHEPTHGGFVGPGMLDAACAGAVFTSPTPDQMFEASKAVDGGAGVFY
                     VVKNYTGDVLNFEMAGELGEAEGIETDYVVTNDDVAVEDSTYTSGRRGIAGTIFVHKI
                     CGAAADDGKDLAGIKELAERVNSNVRSMGMALTSCTPPESGEPIFEIGDDEMEIGMGI
                     HGEPGVERKNIEPADQIVDEMLARILGDSVDFSGSEVAVMVNGMGGTPLMELYVAYAH
                     VADVLEREGVQVFRMPYIGDYMTSLEMAGFSITLLKLDDEMKPYLTAPCDVAAGRPF"
     gene            complement(22303..23826)
                     /gene="glpK"
                     /locus_tag="VN178_03755"
     CDS             complement(22303..23826)
                     /gene="glpK"
                     /locus_tag="VN178_03755"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HWS80116.1"
                     /translation="MAGDYVLAIDQGTTSSRAIVFNHDSATVGEAQREFTQHYPRPGW
                     VEHDANEIWDVTLGVMREALEDAGASPGDIAAIGITNQRETVVMWDKETGEPVHNAIV
                     WQDRRGAQICSDLSEQGYDEMVANKTGLVIDAYFSGSKVRWLLENVDGLKERAQNGEV
                     LFGTIDTWVIYKLTGGRAHVTDYSNASRTLMYNIFDLQWDDELLEMLGGVPRAALPEV
                     RPSSYVYGDTDADVFGAEVPVAGVAGDQQAALFGEVAFDKGLTKNTYGTGSFALMMTG
                     TEPIKSERQLLTTIAWGLGDEPVEYALEGAIFITGAAVQWLRDGLGIIESAEETEELA
                     ASLDSNDDVYFVPALVGLGAPHWDSYARGTIVGLTRGSSRAHLARAALESMCYQTRDA
                     VEAMEADSGTDIPTFKADGGAAANRWMMQFQADILGTPVEVPETLETTALGAAYLAGL
                     AVGFWQDKEELKSRWRLRRRYDPQMSEEERERLYGRWKEAVARAKEWAQEAVESPLA"
BASE COUNT         4229 a         7980 c         7469 g         4526 t
ORIGIN      
        1 gaagattacc ggcgtaggcc gcaccaccga cgcgtacaat ctcaccgacc cggatcccaa
       61 gggcttcggc atcatgcggg ccatgcagct agccgtcgcc gacgccggac tcgaagggga
      121 gcagatcggc tacataaacc cgcacggcac gggtactgtg gccggtgacg gacccgagtc
      181 gcaggcgatg gccaggataa accccaacgt caaggtctcg gccacgaagt ccaccctggg
      241 ccactctttg ggcgcgagcg gcgccgtgga gaccgcgatc tgcgccctgg ccataaagga
      301 gaggaagatc cctccgatga ggaacctcac gaaagtggca gaaggctgcg ccgacctcga
      361 ctacgtcgtg gacgagccac gcgaagcacc cgacctggaa gccgccctct gcgccaacct
      421 cggcgtcggc ggccacaacg cagcagtcgt cctggaacga gcctgatgag cagcatggac
      481 cttccagaga acgtatacga ggtgccacag ggccgacccg agccggcaga cgatggagcc
      541 tgccaccacc tggcaggcat gaggatcgct tccctatccc taacgtccac ggcggaagac
      601 cctgtggtgc gccggctctc cgaacgtacc gcaggctaga gaggacgtac agatgaaccc
      661 cgtcacgacc gacgttttgc acaacaggct aacggagctc gaacagatac tcgccccccg
      721 agggagcgcg ctcgtcgcct tctccggtgg ggtcgactcc tcgctggctc tcgccgtcgc
      781 ggcgcgggcg cttccgagac acagggttct cgccgtcacg tccaacaacg agacctatct
      841 tccgtcggag ttggatctcg cgcgggagtt cgccgagtct ctgggggtag aacacctcgt
      901 cgtgaacacc cgcgagctcg acaacccgaa ctacgcgagc aatcccacga atcgctgcta
      961 cttctgtaag agcacgcttt actctgacct ggcgacgctg gcggagcaga gaggatacgc
     1021 ctgtgtcgtc gacggggcga acaaagacga cgagggtgac taccgtcctg gcaggaaggc
     1081 cgcgagggag ctcggggtcg tctccgtgct ctcggaggcg gggatgacca aggccgaggt
     1141 gagggagctg gcgaagcacc tggacctacc gacctgggac aagccggcgc tggcgtgcct
     1201 gtcgagccgc ttcccatacg ggcaggagat cacacccgag aagctctccc aggtcgcccg
     1261 cgccgaggag tttatgcgca gagagggctt ctcgcaggtc cgtgtcaggc accacgggga
     1321 gattgcccgc ctcgaagtcg gtcccgagga gctcgaaaga gccttcgcca tgagggagga
     1381 gatctccgcc gaactcctcg acgcaggctt cctctacgtc accctcgacc tcgccggtta
     1441 caagtcggga agcctgaacg ccgccttgaa gaagggcaag aaggcccttc ccgtaatctc
     1501 ttcgtagagc atggacggtg tagagggccc gctgctcgcc ctcaccctcg tcttgatgtt
     1561 cgttggcctg ctcggaagcg tcttgcccgg tcttcctggt gttacactca tcttcctgtc
     1621 tgccctcgta tacgccttgc tcacagactt tcgcaccatc ggcgccgcag tcctcgtagc
     1681 cctcttcatt ttcgccgcgc tcgccctggt catcgacttc gtcgctacct cttacggcgc
     1741 ccgccgcttt ggggctagca actggggcac cttgggcggt gccctcggag ggatcgcggg
     1801 cgccctgatc gggctcctct tcctcggcat aggctccctg ttcggcctca tcctcggcac
     1861 catagcgggc gtctttctcg gcgaatacct caagcgcacg cgccaggacc aacaagagcc
     1921 cggttccaca cgatccgact ggcgccgcgc ctcgcacgcg gcaggcggcg tcctcgtcgg
     1981 ctacctggcc tccgccgtta tacagggcct gctaggactc gccagcatcg ttgccttcgt
     2041 gctaggggtg ttgtattagc aggtcagcac gcgccctggc gggcgcttgt cagcacggcg
     2101 cttcgcgcct tgtcagcttg tcagctttcg catgtgcgag tcattgctga gatgctgaca
     2161 agcgaaggct aaaagcctga gcgtgctgaa gtgctgacaa gcggaggcga agcctgagcg
     2221 tgctgacttg ctaatcttct tcgcgccggt gttcgcggag gaaaccttcg agttcctggg
     2281 cgagctcgtc gccggagggc aggttgcgcg gaccctgatc cgcctgggag tcgaagcgat
     2341 cttcgagcat tcttacgtag gaggagagat ccgagtcctg ggagacggcc actgagacct
     2401 gttcctggta ggtctcggcg gtgctttcga ggtcggcggt gtccacctcc atgccgagca
     2461 ggccggagag cttttccagg agcgagaggg ccgcgggggc ggaagggacg gcgggcaggt
     2521 agtgcgggac ggaggcccag aagctcaccg agggcagacc ggcgtcggcg cacgcccggt
     2581 gcaggacgcc ggttataccc gtaggcccct cgtagcgcga ggcggagagg cctaaagagt
     2641 cgaccaggga ggcgtcctgg gagttcgcgg agaccgagac gggacgcgaa tgcggaacgt
     2701 cggcgagcag ggctcccatc gtgaccacga gttccacgtt caacgctttg gccaactcga
     2761 cgacagccgc tgagaaagcc cgccacctga agttcggctc aggtcccgtt agcaagacgg
     2821 ctccccggcc gccggcggcc tccagcctcg gctccgtcgc cgagagcatg ttttcgggcc
     2881 attggatctc gcgcgtcacg ccgtcgacca gctttacctg cggccgcgtg gtctggaagt
     2941 cgaagaactc ttcgccgtcg aagccgccga aacgccgcgc ctcccaggag ctggccaggg
     3001 ccccgacggc gacgctcgcc gcatcagcag cgtcgttcca gcccgtgaag ccggcgatga
     3061 gaacaggtct ctccagctcg ggcgtccaat cgatgtcgat gtgtcgtata tccatggagc
     3121 aagcttacca cgcggcaagc gccgagccga aagaacattc ctctatgcaa aggagcactc
     3181 cagggtcagg gcgtgctgca gccgcctgag gtactcctgt tcggggatct cacgcgcccc
     3241 cagcctggcg agatgggggt tctggatctg gcagtccagc agtgcgtacc cccgctcttg
     3301 cagccggttc accaggtaga ccagacagac tttggaggcg tctctaatgc gggtgaacat
     3361 cgactcgccc ataaaagccc cgccaagggt gaccccgtag agaccacctg cgagctcgcc
     3421 acgcctgtag gcctcgacgc tatgagcctt gcctgcgtgg tgcagcccga cgtaagcgtc
     3481 gatgatctcc gggctgatcc aggtctcctc cctgtcggca cacgccctta tgacgccgtc
     3541 gaagtcctcg tccacgcgca actcgaaggt tcccttgcgg attacacgct ccagcgagtg
     3601 ggggacgtgc aggtcttcga actcgagcac gcttcgcggg tctgaccggt agaattcgat
     3661 gcgcccgtag gcatcggcca tagggaaggc gcctgccctg tagcatgctt cgaggatctc
     3721 cgtggtcagc ttcataagga tggtagcggt gccacggtcc tatgcagacg accccggcga
     3781 gtcggaccat gagaaggaca tgtagttcat ccgggagcct ggatcaaggg ccggatctca
     3841 ccgactttct caccgtgtag ggcgcgcacg ctggagaccg cctcgggaac ctcgacgccc
     3901 atgccaccga gcacggcgag cgccgcggga cgcacggccc tgaccgcacc gtcggagatc
     3961 ttcagcgcca tcccccagcc ttcctggctt ccgacagcga gcatggcttc ggccccgccc
     4021 ttgacgagaa ggtccgtcga gtccatgagc tcggtgtcga atcttcccgt acccgcgacc
     4081 ataaagggat gctccctcat cgcgtccccg atgcgcaggg ccgcgagggc gagttcatcg
     4141 gagagggctt tgcccgtggc gatgcgggcg aggcctgtcg caaagctccg cagcggcagg
     4201 gcgaaggccg gcacgccaca gttatctccg gcgacgagca cctcatcatc ccgcaggccg
     4261 cagacctcgg cgatgagacc gagtatgcgg cgttggagcg ggtgggcggg gtcgcggtag
     4321 gtggctgtat cgtagccttc gtggacgcag acggcgagca tccccgcgtg cttgccagag
     4381 cagttgccgt ggatcggacg cggttcttcg ctactgcgga tcaacctgtc tgcctctggt
     4441 tcgtacagcg gcagatgggc gccgctctgc aggtcgtcct cggtcaggcc ggctttctcc
     4501 aaaatggacg tgacggcggt gaggtgaggc tcctcggcgt tgtgcgaagc gcagactacg
     4561 gcgagctcat tatccgtcag cccgaaggcg tcggctgcgc ccgagcgtac gagcggtagc
     4621 gcctggaacg gtttggccga ggagcgggcg tagatgtagg cttcggcgtc gccagcagcc
     4681 tcgagtacct tgccatcggg atcgcagatg acgagacgcc cgcggtggac gctctcgaga
     4741 agcgcgccac gagtgacggc agcgagcggg gcgtcctccg gaagagcggc ctcgccaggg
     4801 acctggctat tcatccgaga gacggatgat gtgtagggaa agcaggtcgt gggccacccg
     4861 gatccggccc atcgcgacgg agaagtcgcc acccgccagg tactcgcgcc cctccctgac
     4921 gagggcgcgg agcttctcga tcagttcgtc gtctttgccg ccgggactcc cacccctcag
     4981 gctcgcttcg gccatgccga gcatggcgtc gaccctcggg ctcgctgtcc acattgcgag
     5041 gatcgcgatg tccatgtagc tgtcgatcct gagcagatga gcagtcctca gcgccgcgcc
     5101 ggccctctcc cccgccctca aggaaaccac cgccttatgt tctccgagag tatcctaggc
     5161 agggtctcct caggcacgcc cgcagcctcg gccgccgcga ggatggaatg caacgtcgac
     5221 ggcaggtcgc cgtagggtat gtccgacccg aagcagatcc gggaagggtc gacggagcag
     5281 cagagttcgt acagatcttt ggcctgaaca acggaggttt cgaacagcac gttcgggtgg
     5341 ccctcgaagg cgcgggtggc cgagagcacc tcgaccatcg cgcagtggcc gaggatcagg
     5401 cgcaggccgg ggtgcgcctc gaccgtgggg aggagcggct cgacgatgcg ctgcatggcg
     5461 aagccggtgt ggatcaggat cggaagatct gtctccgccg ccattccgaa aaggcgcacg
     5521 accctcggat cgtccagttc gaactcctgc gagacgggat ggagcttgag ccccataaag
     5581 ccgcgctcga cgcagcgaga gaactccctg tcgtaatcga ggtgcgggtt gaggcggaaa
     5641 aaaggcacaa aggtggaagg gtactcctcg tatgccctcc agacaacgtc gttcgggccc
     5701 gagaagtcgt cgcgggcgtt cgggtcgttc aggggaaaca cgatgctctt cttcacccct
     5761 gcggcctcca tgcgctcccg cataccgtct ccggtcatcg tcctgccgtc gacgtccgta
     5821 ccgatgtggg cgtgggcgtc gaagacctcc acaccggcgg ggatcttcgc cctgacccag
     5881 gaccagatag aggccatagc cccgcccgtc agcgcctcga tgtcgtgctc catctgcaaa
     5941 gtgaccatca tcctaagact aacgaccgtg caatggtacc agggggcatt ttctgatgct
     6001 cgggtaaccg cccgcatcgg tggtgcctgg taccatgacc attatcgtcg ggacgtgaga
     6061 ggacggtgga gtgtaatgat cgcatgggtg ctgcatatcc tggagctgat cgcgggcgta
     6121 ttcggcattt cgatagggtt ctcacgcttg cctaacgccg cttcagcctt gcaaatcgtt
     6181 acccttaccg ccgtcggtct cgtcggactg tcggccttcg taaggcactt tatcttccac
     6241 aagagcgatg ccagacgcct cgggtgggac tcgaccaggc cggagttcca gtacgaagtg
     6301 gggttcgcca acctgggctt cgcgttggtg gcgttcttcg tatacttcgg agagtgggga
     6361 ctcgctcccc acgttgcggt cgtactcggc tacggcctct acctcttgca ggcggcgctt
     6421 ctccacacct ggaagtcaat aagcggggac ggcggtgtgc gccgctttct ccaaagcgcc
     6481 ttacccgcgc tcatcttcag ttccgtgatg atctacttcg ccgtaaaggg aatgatcgca
     6541 gcgaaccttt ccgtcctctg actcggaaag gacctcgcaa cccatcaaga gcctgagacc
     6601 ctgctaacat tcccattgga ggcggaagga gaggagtcag agtggagata cccaaagcca
     6661 tgatcgtaga gcgcatccgc agccaacagg gtgccgagaa ggccaaagag gccgacagcg
     6721 agctccccga caaggtggac accgaagcca actccgacct gctccagaaa tacggcctgg
     6781 atcccggcca gttgaccgac atagcgggcg gcgacccggc cgtggggtag cggccgaagg
     6841 cggatctcgc gcaactcgct acgggcgaat caacaaactg acttgcttgt aaagaggttc
     6901 gtccgctttg cgattcctac ggcgcgccgt caagcagacc ccggcgcgcc gcctcgtgca
     6961 tgacttcgcc cacgtttatc gttaacgggc cgttcggctc gaggcgcacc ttcacctcgt
     7021 tgtgtggtcc gatctctatc tcgcgctcgc cgtcgagcgc caggctcccg accttcggct
     7081 ccagctctac cgactcgccc ggctcgacgc gtctcgactc gacgacgccc acaggagcaa
     7141 tgagaccagg agcgagcggt acagtgagga ccgtatcggc ctccaccgga ggggcgagcc
     7201 gaacataaag accgtggggc gtcgagcggt ctaccggatc caggaggccc gctatggaag
     7261 agagtcccac ggcttcgggc gaggcggagg cgactactac ctccgagatg tccggggccc
     7321 gccacagggc gcgcgccccg acgaaccgtt ccgagtttaa ggccacttcc acgagggcgc
     7381 agtcgcggtc tggatcgccg tcgatggaga cgtacaggat cttctcccgg cgcaacacat
     7441 cgtccccgac tcgcccggtc gcgaccagcc cggtcgccag tcccgcgacc gtcgcctcct
     7501 gcatcttggg gaaggtgttg ttggtgccgg tggagagcgt gcagaggggg atgtggggat
     7561 agcagtggcg ggcgacgacg cggttggtcc cgtcgccgcc cagcacgacg atggccgaga
     7621 cgccccgctc gcacatctcg ccgacggcgt acgccgtgtc tcgcgcgccg tgccggatct
     7681 tcatctctac cagctcgagt tcgggcaagt cctgcttgcg ctcgctggcg gcattggcgc
     7741 gccggttgcg gtccagtccg tcgaaaagcc ccgaggcggc gggcatcatc agaacacgct
     7801 cgacgccaac cgccgcgagc cccaccatca gccgaaagac catgttgccc ttctcggcgt
     7861 tgtcgaagac cgaagctccc gctaccaggc gccggatgtc ccggcccgaa gcggggttcg
     7921 cgacgatgcc gaccgtcgtc atagggtggg agtcttactc aaggccgtcc agaaatcccc
     7981 cgatgaggcg attcgtccgt cccgccgact ccatctgcgg catgtggcct gtgtcttcca
     8041 ggatctcgat tctgacgccg tcggacaggt tctcggtgtg ggaggccggg atgatctggt
     8101 cgtcttgtcc ccagatcacc agtacgggcg tctcgaggcc ggagagatcg ggcccttcgg
     8161 cctggctgcc gtccgggaac atcttgtcgg caaccgcgcg caaggcttcg tccaccccgt
     8221 ccatgcgctt gaagcgcagt tcgtcgttga cgagccggcg tgtgacgagg tcggggtccg
     8281 caaacaggag ccccagaact ctcttcatct ccctgcgccg gttcgcggcg ataaaaccct
     8341 cgatgtagtc ttcgttgatc tcctctccga ggccggcgct cgcgatgagg acgagcgacg
     8401 cgaccttgtc gtggtgtgcg agcgcgaacg agccggctac cgcaccaccc atcgaatgcc
     8461 cgaccaggtg cgccttctcc acacccacct cgtccataaa gccagccagg atctcgacga
     8521 aggagtcgag gtcgccctcg ccaacgtcct tggaggactg gccgtggccg ggcaggtcga
     8581 gggcgtaaac ggtgcgtccc gcctcggaga gggcctcctg gttgaagacc cagatgttta
     8641 tgtcgccgcc gaagccgtgc agaaggatga ggggaggacc ttcgccctcc ccgatcttca
     8701 ggtactgtac gttcctgccg ccgatctcga ccgtctgcgg ttcgggaccg ccggcctccg
     8761 ccgccgcctc ttcggggtcg aagctctcca ggaactcctc gacgaaagaa tctatctcct
     8821 cgtcggggac ctctgacggc gcgatcacgc cgagtagacc gcccacgggc aggacctccc
     8881 cctccctggc gacgcggcgg cgaaggacgc cctcggccgg agactcgata gcgttgttga
     8941 tcttctcgct ctcgacttcg acgacctcgt cgcccttgga aatctcggtc ccctcctcga
     9001 tgagccactc gacgacggtt ccttccgtca tcgagaggcc ccacttgggc atccccagct
     9061 tggagatctg ttgcgtctcg ctcacgcccg cgcccccgcg ccggtgacct cgcgcgccgc
     9121 ctcagcgatc cggtcgggat cgggcacgta caggtcctcc agcgaggggc tgaacggcgg
     9181 cggcgtgtgc ggtgccgtta ccatcttcgg cgccgccttc aagtcctcga agcagttctc
     9241 ggccacgcgc gccgcgatgt cggtcgccat gctgcaacgc gggttcgact cgtcgaccac
     9301 gaccagccgc ccggtgttct cgacgctctc gaagatcgtc tcctcgtcca gcggcgaagt
     9361 cgtcctcggg tcgacgatct cgcactcgat accttcgtcc gcgagcgacg cggcggcctg
     9421 ctcggccatc gagaccatgc ggcctatggc gactatcgtc acgtcctcgc cctcgcgcac
     9481 gtactcggcc tcgcccagtg gtatcgtgta gggctcctcc gggacctcgc cttccacagc
     9541 gtagagaacc ttgttctcga agaacataac ggggtcgtcg tcccggatcg aagctataag
     9601 gagtcccttg gcgtcgtagg ggttggaagg gatcacgacc tttaggccgg ggatgtgggt
     9661 gaagatgggg tacaaagact gtgagtgctg gctggcggcc ctgataccgg ccccgaacat
     9721 ggtccttatg accatcgggg tcttggcctt accgccgaac atgtagcgaa acttcgcggc
     9781 ctggttgtag atctggtcca tgcagacgcc gaagaagtcg acgaacatga gctccgccac
     9841 gggacgaagc ccggagcagg ccgccccggc cgccgcgccg ataaaggcgg attcgctgat
     9901 cggggtgtcc aggatacgct ccgggtattt ggcataaagc cccttggtaa cgcccatgac
     9961 gccgccccag gcgtccatct cccccggtga gccggctccg ccgacgttgt cctcgccgaa
    10021 gaccaccacg gtctcgtcgc gctccatctc ctgcgcgagc gcctcgttga tggcctgcat
    10081 gtagctgatc gtgcgcgcca ctttgcctcc cttcggtcgg cgctataagc tgtcagcatt
    10141 cagcactcag cttgttgacg tggcttgcca tgggccaatg tccgctgact gctgatggcc
    10201 gatggctaat agctaacgta aacgtccgtg agcaggttgt cctgggacgg gtccgcggcg
    10261 gccttggcct cgtccacggc ctcatcaata agctgctcga cttcttcgtc gatcctgtcg
    10321 agggcgtcct cgtccaccag acccgccgag gtcacgcgct ggcggaacag ggtcaggcag
    10381 tccttctcgt tgcggatctc ctctacctcg ttctcgccgc ggtaggtctg ggcgtcgccc
    10441 tcgaagtggc cgtggtagcg gttgaccttg cactcgatga gcgaagggcc gccgccctcg
    10501 cgggcgcgcc ggatcgcctc gcccgcggcc tcgtacaccg cgaagaagtc gtggccgtcg
    10561 acggtgacgc cgggcatgga gaatccatct gccctcttgg cgatgtctat gcccttgctg
    10621 tggaacgcgt gcgcggtagc ctccgcgtag ccgttgttct cgatgacgaa tacgcaaggc
    10681 agatcccacg cgctcgccag gttgagactt tcgaggaacg tgccctggtt ggcgcctccg
    10741 tcgccggtga acgagacgcc cacctgcctg gtgccgcgta tcttggccga gaggccgacg
    10801 ccgcagacca acggtggacc gccgccgacg atgccgttcg cgccgagcat gcccttgtct
    10861 aggtcggcaa tgtgcatcga gccgcccttg ccgcggcaga tgccctcgtc cttgccgtag
    10921 atctcgtaca tcatcccctt gacgtcgcag cccttggcga tggagtggcc gtgcccccga
    10981 tgtgtgctgg ccacgtagtc gttctcgtcg aggtgggcca taaaccccgc cgcgatggcc
    11041 tcctctccgg cgtagaggtg cacgaagccc gggatctcac ccgtggcgaa ttcggtgtgc
    11101 agcctatcct cgaactccct gatggtcttc atgatccgat acgcttcgag caactgttcc
    11161 ttgcccaact cgcgcgcccc cgcgacctcc gtcattgggc ctcctctctt cgacgcttcc
    11221 ccaagcaaca gtccacaata tacgggccga atttccgcgt caagcgtggg atggtactcg
    11281 gtgccagggg ggaatcgtgc gggcgtggcc ggccgcaatc agggtcccgc acgtgacatc
    11341 ctggttggga cagagccttc gtatcgcagc gaggacgttg gttctttgtc aggttcagat
    11401 atgcgctaga gaaccctgct gcggattcta ggaagggtcg ttgcgttgga gggtgagcat
    11461 ggagacgcgg gggccgtcga cgctcttgac ttgcatggtg acgccgtcgg cctccacggt
    11521 gtcgccggtc tcgggggggc gacccaggga gcccaggatg tagccgccaa tggtatcgaa
    11581 gtcctcgtgg ggcaggtcga tctcgaaacg ctcgttcacg tcgattatgg ggatgcggcc
    11641 gtcgaccgcg tagaggtggt cactgatctt ctctatgaca ggctcaccct cgtcgaactc
    11701 gtcctggatc tcaccgacta tctcttcgac gatgtcttct atcgtgataa gcccctcgac
    11761 gctcccccac tcgtcgataa caatagccat ctgcagcgtg cgggtctgga actctttgag
    11821 gatctcctcg atgcgcttgt tctcgggtac gaccaggcac tccctgatca cggaccttat
    11881 gtcgaagtcc tcgtcgttcg tgcgggcggc ccgaaacagg tccttcacgt ggaccgcgcc
    11941 gaggatgtgg tcgaggtctt cctcgtagac cggatagcgc gtatagcgcc cgtagacggc
    12001 ggcgtcgacc agctcgggga gcggggcgtc gctcgcgatc gcctcgatgt caggcctggg
    12061 gaccatgatc tcgcgcgcca cccggtcgcc gaagtcgaag accttgttca ggtagtcgcg
    12121 ctcctctggt tccagcacgc cagaggcggt ggaggaggag atcatgatcc tcagttcctc
    12181 ctctgagtgg gcctccatct cctcccccag ccttatgccc cagggcctca agacgaagtt
    12241 ggtcgaagcg ttaaccaccc agaccacggg gttgaagagg atcttgaaca ggttcaaagg
    12301 acggctgatc cagagcgaca ccctctcggc cttctggatg gaaaagtact tgggcgcctg
    12361 ctccccgaag accaggtggg cgtaggtgat ggccgagaag cccaagatga tggcgataat
    12421 ggagactacc ccctcgtttg taatccctac ggcctcgagc acgggacgga taaggtgcga
    12481 gacggcaggc tcgccgaccc aaccaagcgc cagggaggct accgtgatcc caacctggca
    12541 aacggagagg taggcgtcga ggtcgcgcag cgtctctcgc acgagggccg cccgcgtgct
    12601 gccttcctgc tccagctgca agatgcgcga ttcccgcacg cgtaccagcg cgaactcggc
    12661 cgcaacgaag aaaccgttca ggcccacgag gacgagggcc aaaaagagat taaaccagga
    12721 taagaggggt tccggcactt ctacgcgggc ccaccggcgg tcgggccgca aggtcttcgt
    12781 cggtggtgtc tagagtgagg ttcgtccatc gcgttacggg gcgaattata ccgcagccca
    12841 ctgcccgcac aaatctagga acacgatagg aggcccggcc tcggtggccg gacctctcag
    12901 tgtgtcgttg actgaaggct atatctacgc gaggccttca gcttcgtact cgcgcaggaa
    12961 gccttcgaag aggcgcttgt gaccctgttc gtcgtgcagg atctctatga ccatatcctg
    13021 cgtaacggga tctacttcct cggtctcctc caccatgcgc gtgtagtgct ggatggcgcc
    13081 ctcttccgcg tctattacac cctttatgac gtgtacgatg tctacctggt catcgggcgg
    13141 ctggagcgtg tcctgcgacg ccttgaactc catcgaacca ggcacgacac catagagctc
    13201 cttgatgcgg gccgcaaact gctgggcgtg ggtgagctct tcctgtatgt cttcctctat
    13261 gctctccttg acctcctggg cgcggacgcc atcggggttt atggagttcg ttacgtagtt
    13321 catgacggtc tcgagctcca taaagtaagc cgtcttcaac agctcgacga tctcctcgcg
    13381 cttccctgac atctcttcgg agagaatacc ctcgctgccg cgattggtct ccatgctctg
    13441 tgacctcctt caagatcgca tgagatacac agagaatgta ccctcgcacc ccccacttaa
    13501 acgtgaactg ggaaacatga agacgcgccc ggagagtctg cccccgggtc cacgcatgcg
    13561 ggacccctct agctctactg cagcttcggt tccgtgcggg cttccatcgc ggcctccacg
    13621 cactcttcgg cgtccgcgcc ggtcgaggcc gtgaccccgg ccgcgaaagc cccttcgacg
    13681 aatggggcgt cgacgagcgt gaccttctcc ctctcttgcg ggggcagcat ctccaggacg
    13741 gtctctgcgg agaggacggc gctgccgagg tccatgaaca cgagcacctc gtcggtttcg
    13801 aggccgcgaa tggaggcttc gatcccctcc gggtccgtgc cgaaagagcc gtcggcgtcg
    13861 cctccgacgg ccgagatctc gacctctcct gccatctgca agaccagatc cgccgtgcca
    13921 tcggcgatct ccttgctgtg cgagacgagc acgatcccga ccatcaggag gaaccttcca
    13981 gcacccgcgc cgcggcgtcc aggagcatgt acgtggaggt cgcccctggg tcctgatgac
    14041 ccgcggccct ggcccccaga tagctcgccc tccctctctt cgccgtgagc ggaatcgtcg
    14101 actcggcccc ctccttcgcg gcatccgccg cgctcctgaa cacccctgcc acgctccctt
    14161 cgccggcggc ttccttcgcg gcttcgacgg cgggttcgag ggcgtccacg atcgtcttgt
    14221 cccccacctc ggccttaccg cgctgcttta tgccgccgag tgcggcttcc agagcctcgg
    14281 cagcgtcatc ggctaagacc tcttccttgt cttcaagcgc cgaggaggcg cgtaggaagg
    14341 cggtgccgta gagcgggccg gcagccccgc cgaccttgct gatcagggcc atagatacgg
    14401 ccttcagtac gtcggcaggc gtcgaagggt tcgcggcttc gagacgttcg agcgccgcct
    14461 tgaagccgcg gttcatgttg ttgccgtggt cgccgtcccc gatctccgag tctagcttgg
    14521 tcagataccg ccggttctcc tccatggcgg cggccatccc cttcaccaca tcgctggcac
    14581 tttgcgtcgc gtccaaatcc tacacctccg gtttgcctaa tccttcactt accttgtgct
    14641 tgagtacctt cccaagggca ttgcgcggca ggtcttcgac gatcaccacc tcgcgaggct
    14701 tcttgtaaga agccaggtcc tcgcggcaga aactcgtcac cttctcggcg gtaagacccg
    14761 ggtcttcggg caccacgacg gcgaccacgc gttccccata ctcggggtcg ggcatcccga
    14821 cgacggccac ctcggacacc ccgggacaac cttcgatcac ttcctcgacc tcgcgggggt
    14881 agatgttata gccgccgctg attatgagct ccttggcccg cccgcttatg gtgaagtagc
    14941 cgtcctcacc gacgtaaccg agatcccccg tggcgaacca gccctctccg tcgaagctct
    15001 cctcggtcgc atcgggcctg ttccagtagc cggggaagac gttcgggcca cggacctcga
    15061 tctccccgat ctccccgtcg tccaaaggct cgcgcgtctc tacgccaacg acccgcgcct
    15121 cctggcccgg aaatggccgt cccacggtac ccggcctgcg ctcgccttcg taggggttgg
    15181 tgaggttcat gatcgtctcc gtcatgccgt agcgctccaa aatacgctcc ccgaagagtc
    15241 cctcgaactc atcgaaggcc tgtgggctca agggagcgct gccagagacg tagagccgga
    15301 tcggatccgg acgctcgtct cgaccctcag cctccctgag cagccgcgtg tacatggtcg
    15361 ggacgccgaa gaacatggtg accctccctg cgagcagggc atcgtaggcc gcagccgcat
    15421 cgaatttcct gtgcagctcg gcgcttgcct ccataaggaa cgtgccgtgc gccccgacca
    15481 tcaggccgtg cgtgtggaac agcggcaggg ccagaaacaa cgtatcttcg cccgtccagc
    15541 gccaggcctc gcatacagcc tgcgcattgg ccacgaggtt gcgatgtagc agcatcgccc
    15601 ccttgctgcg tcccgtcgtc cccgaggtgt aggcgatcac agccaggtct tcgccacgcg
    15661 gcatctgtgg ggcgtgcgtc tcgccaagga agtcttcgaa cccctggccg gtctcgatca
    15721 ccgcttccag ctcggacaga tctaggcgcg tgcgttcgag ttccggctta cgctcctcgt
    15781 cggtaaggca cagacgcacc cctgcgtcgc cgaagatgtg cctgagctct cccctgcggt
    15841 actggctgtt cacgggcaca atgacgcccc cggccaggtg cgtccccagg tatgcagcca
    15901 aaaggtccgg gtggttaccg agatagaggg ccacccgatc gccggggcgc agtccccagg
    15961 cggagaggcc ggcgccgaag ccctcggccc gttcgcgcat gccctggtaa gagatctcct
    16021 ctccctcgaa gcgcaagcag agcttgtcgg gtgatctctc ggcgcttctc acgaacgcct
    16081 ccgcgatcga agccgggccc ttctcactcg cgcccatggg tttcctccaa cattcggtgg
    16141 ccggtacgct cgaagtaggg tacatccacg ctccgatctc gcgcaacggg aagcgggaaa
    16201 gggcccgccg caacatccgc ttatgacctg ttgacacgct gagtccaaag ctctattctc
    16261 ggcgacgacg gcaggggcgc tcttccgggc gtcaaggcgg cgggaaaggc gggcagctag
    16321 agtcgccggg tgtcgcaatt tacgcctctg atgtggctta cataggttct cgccgaggct
    16381 cgcgacggca gggtggcgga gaaggggttt tccgatgcgg aagaagtatt ctcgtcgtag
    16441 ctttctcaag atgacgggtg caagccttgc gggggcttcg ctcctcggcg cagtggggtg
    16501 cggcgggtcg gcatcttcct tttctccgaa cgcagagatg tggaagcagt actcggggac
    16561 cacgctcaac ttcgtctcgg agaacacggc cccgaccacc gccatagcag cgaacctgaa
    16621 cggcttcaag gagctcacgg ggataaacgt caacgttttg cagctggagc tcggcgcgct
    16681 cgttcagaag gtcgcgctcg acatcggctc gggggagggc gcctaccaga tcgtctacgc
    16741 cgatccctac cagatcatgg ccccctacca cgccgcgctc gccgacctga atgagttcga
    16801 cggcgacgac gatctacctt ccatacccaa gggcacagac gactttatcc cgacccagct
    16861 cgcggccgca gggcgcttcg agaacgagca gaacctctac gcgctcccct acgactgccc
    16921 gaccatgatc tggatgtacc gcaaagacct cttcgacaag tacggcgacc ggatgagcca
    16981 ggacctcggc ttcgaccctc agccctcgcc atccttgacc tgggagcagt actaccagat
    17041 agccgactgg ttcaacgaga accaggatga ggtcccttac ggcacgggcc atcaggccag
    17101 gcaatacgac tccctgatgt gcgacttctc caacatcttg tggtcctacg gcggcgacta
    17161 cttccacagc aaggagatcg ggagcctggg gacgaccgac ccgggcccgt gcacgctcga
    17221 cgagccgaat gcgctggagg ccgccgagtt ctacaagaag ctgctgtcca tcgcccaccc
    17281 gggcagcgtc tcgtgggact ggaacgccac ggacgaggcg ttcaggaccg agcaggtcgc
    17341 gatggtaccg aactggcacg agttcgccgc cgtggaccag gaccctgccg agtcgacgat
    17401 agtgggcaag atcgggtact ctccgctccc caaaggccct gttagaaacg cgaacatgtg
    17461 gggcgggacg ggcgtcggca taaacggcgc ctccccgatc aacgagcaga aggcggcctg
    17521 gctgtttctg gtctgggcca cctcgcccga cacgatgctt atgggcttga agagcgatgt
    17581 cgggggcggg accccgacac gggggtccct ctacgaactt ccggaggtga agaaggccat
    17641 ggaaccaccg actaacctgc cgaacctgct cacctacgac gccgtctccg aggcgtggcg
    17701 gcccgagaat atcgggctca ggcccaagat ccaatcgtgg aacgaatgcg acacggccat
    17761 ctacaccgaa gtctccaaga tgctggccgg ccagaagacg cccgagcagg cgatgaagac
    17821 ggccaagaga tacttcgacg aggcgcaggc cagggtcgag aacctcgcct ccgggccaac
    17881 gggggtcgcg taatggccgc cacacagaga cgcagttccg gcgccacgaa gggcgccaat
    17941 ggcgggggta ggatctcgct ggagatgaag atgctcgtac cggtgctcgc gctgctcgcg
    18001 gtcctctcgc tcttcccgtt cttctacatc atctggatga gcctgaacat cgtctcgctc
    18061 ataaacggca tctccttcga cttcgccggc ctcgccaact ggacccgcat gttcacggac
    18121 gagatcgtcg gcgcggcctg ggcaagggcc gcgctgtact tcgtggccac cgtagggctc
    18181 gagatgatcc tgggcatagc catagccctg ctcgtgcacg agctagtctg gggccagaac
    18241 ctggtgctct cgctgatact gctgccgatg ttcatggcgc cggtcatcgt ggggctcctc
    18301 gggcgcttcc tcacggactc ctcctacggc ctgtacgcct acgtgttgcg cgagctcggc
    18361 atcttcgaga gcgacatact ggggaccgcc agctcggcct tgccggccgt aatcctgatg
    18421 gatgtctggg agtggacgcc gttgatcgtc ctgatcgtgc tcgcgggact agcctcggtt
    18481 aaccagtacg tgatcgaggc cgccaaagtg gacggggcag gctacctgga gcggctgcgc
    18541 tacatcgtgg ccccctccat agcaggcgtc gtgatagtgg ccctgctcgt ccgttcgatg
    18601 gacgccatcc gctacttcga catcatcacc gtaacgacca acggaggacc gggcaacacg
    18661 accaagatca tccccataag gctgtacgag acggcgttcc gctttttcga cctcgggtac
    18721 gcggcggcca tcgggctcgg gatgctggtg ttctcgatcc tggtggccaa cgtgttcgtg
    18781 cggatcctcg agagcagggg gcttgcgaga tgacgacttc tactacgggc cgcaattcgg
    18841 gcaaacaggt aatactctac gccatcctct tcctcgtgct gctgtggacg ctggtcccga
    18901 tagtgtggat ggtcctcgcg tcgttcaagc ccggggtcgc ccaactctct tcgacgccgg
    18961 tcttcgtctt caagccgacc atgcaacact acgtggcatt gtttaccggc ggaaacctcg
    19021 gggcctactt ccttaacagc gtgctcgcag ccggattctc gacggttatt gcggtgtccc
    19081 tgggctgcct agcgggttac gggctcgcca ggagccactt ccgtggcaag caacacgtgg
    19141 cgttctggat catctccacg cgcatggccc cgatagcggc gatcgtgctt ccgctcttca
    19201 tcctttttcg gtacgtgaac ctgctcgaca ccataccggg cctcatcgtc gcctacctga
    19261 cgttcaattt gccttttgcg atctggatca tgaacgcatt cttcatggat ctgccaccct
    19321 ccctggaaga agcggcgatg gtcgacggcg ccacgaggtt ccaggctttc taccgggttg
    19381 cccttccgct ggtcacgccc ggtatagtga cgacggccat actgtgtctt gtcttctcct
    19441 ggaacgacta cgctttcgcg gcgaccttcg ccggacccga cagccagacc ctccccatcg
    19501 ccgcagccca gctcaacacc cagacgggcc tgaactgggg ccagctctcg gccatcggca
    19561 cgatcgtcgt gctccccatg atggtcgtcg gcctcgccgt gagacgttac ctggtaaagg
    19621 gcctcacgct cggcgcggta accggagagt aagagccgct ctggaaagta ctctcgctac
    19681 gcactgcgca tcaaaacagt gtgtagcccc cgtccacgac gaggatggag ccggtcatga
    19741 aactggaggc gtcgctcgcc aggtagacga tgctaggtcc cagctcttct ggcgtggcgt
    19801 agcgctgcat gggggcgtct tcgatccagt gccgccggaa ctgcggctcg tccacagggg
    19861 acatggccgt cttgacgtag ccaggagcga gggcgttgac gcgcacgttg tagggcgccc
    19921 actccgcagc cagagatctg gttaactggt ggacggcggc tttggaggcg ttgtaggcgg
    19981 gctgccactg cgggcggttg acgatcatcg cggagatcga accgatgttg ataatggtgc
    20041 cgccgccgtt ctggatcatg cgcctcccca cgacctgggc gcagtgccag agcccgtgga
    20101 cgtttacgtc gaagacggcg tgccactctt ctaacggcac ctctgcggca ggcctgtggt
    20161 aacagaggcc cgcgttgttg accaggatgt ctacgggccc gagccgctcc gtcaccgcct
    20221 cgagcatccc gtccacctgc gcggggtcgg tgacgtcgag gtcaacggcg atggcgttgc
    20281 cgcctgaggc gtggatctcc tcggccaccg cctccgagcg ctcgcgggta cgggcggcca
    20341 cggcgacgcc ggctccggcc tcgacgagtg ccttcgcgaa ggcctggccc agtccagtat
    20401 tgccgccggt gacgacggcc gtcttgcccg taaggtcgaa ggcatccaga acggccaatg
    20461 agcctcctct gttctctatc cgggcggttt cgtggggagc cctattcagg ctccccacgt
    20521 tggatctcat ttcgctatca gaacagaatg acccacgtca cagcgccgag gataccgcct
    20581 atgatagggg ctacgacggg gatccaagcg tacccccagt cgttaccgcc tttgcccgca
    20641 atgggcaata ctgcgtgggc tatacgcggc cccaggtcgc gcgccgggtt gatagcgtag
    20701 ccggtcggac cacccagtga gagtccgatg ccgaggacga gcaggcccac gagcagagga
    20761 ttgatgccgc tccctatgac cgtcgcggct tggccgctga ccgcccccgc gttcgcaacg
    20821 atgccagaga cgccgaagac gagcacgaag gtgccgatga tctcggttat gacgttcgcg
    20881 ggagtgttgt agatcgcggg tgcggtgcag aacactccga gcttgagtcc ctcgtcttca
    20941 gtctcaggcc agtgcggcag ataggccagc cacacgatga cggccccgac gaaggcaccc
    21001 agaaactggg cgatgatgta gggaattacg tctgccagcg ggagccctcc cgttgcggcg
    21061 aaagcgatgg tcacggcggg attgatgtgg gcgccggaga actggcctac gcaatagacg
    21121 gcgatggcga cggccatccc ccatccgaag gtgatgacga tccagcccga gttctcggcc
    21181 ttggagttcc ttagcagcac cccggcgacc acaccgtcgc cgaggatgat caggatcatc
    21241 gtcccgataa actcaccgat gtacgcttgc atctatcact cccttctaga aaggccgtcc
    21301 ggccgccaca tcgcacggcg cggtcaggta gggtttcatc tcgtcgtcga gcttcaggag
    21361 cgttatggag aaccccgcca tctccagcga ggtcatgtaa tccccgatgt agggcatcct
    21421 gaagacctga actccctcgc gctcgagcac atcggcgacg tgggcgtagg ctacatagag
    21481 ctccataagg ggcgtgccgc ccatgccgtt gaccatcacg gccacctcgg agccggagaa
    21541 gtcgacggag tcgccgagga tcctggccaa catttcgtcg acgatctggt ccgccggctc
    21601 gatgttcttg cgctcgacgc caggctcgcc gtggatgccc atgccgatct ccatctcgtc
    21661 atcgcctatt tcgaagatgg gctctccact ctccggcggg gtgcaactcg tgagcgccat
    21721 ccccatggag cgcacgttgg agttgacgcg ttcggcaagc tccttgatgc ctgcgaggtc
    21781 tttgccgtcg tcggccgcag cgccgcagat cttgtgcacg aagatggtgc ctgcgatgcc
    21841 acgccgcccg ctagtgtagg tggagtcttc gacggccacg tcgtcgttgg tgaccacgta
    21901 gtcggtctct atcccttcgg cctcgccgag ctcccctgcc atctcgaagt tgagcacgtc
    21961 gcccgtgtag ttcttgacga cgtagaagac cccggccccg ccgtccacgg ccttactcgc
    22021 ttcgaacatc tggtctgggg tgggactcgt gaagacggca ccggcgcagg cggcgtcgag
    22081 catgccgggt ccgacgaaac cgccgtgagt cggctcatgg cccgagccgc cgccggagac
    22141 gagggccacc ttgccctcta tgggggcgtc ggcccgcacg acgacgttgg cttcggggat
    22201 gacccggatc aggcctccgt gcgccagctc catccccctg agcatgtcct cgacgaccct
    22261 ttcgggcgcg ttgatgactt tcttcatgtg cctgattcct ccttaagcga gcggggattc
    22321 gaccgcttcc tgcgcccact ccttggccct tgctacggcc tctttccacc ggccgtagag
    22381 ccgctcacgc tcctcctcgc tcatctgcgg gtcgtagcgg cgcctgagac gccacctgct
    22441 cttgagctct tccttatcct gccagaaccc gacggcgaga ccggccagat acgcggcgcc
    22501 gagtgccgtg gtctccagcg tctcgggcac ctcaacgggc gtccccagga tgtcggcctg
    22561 gaactgcatc atccagcggt tggcggcggc ccctccgtcg gccttgaagg ttgggatgtc
    22621 cgtccctgag tcggcctcca tcgcctccac ggcgtcgcga gtctggtagc acatgctctc
    22681 cagggcggcc ctcgccaggt gcgcccggct gctgccacgc gtaagtccta cgatggtgcc
    22741 cctggcgtaa gagtcccagt gtggcgcgcc gagtccaacg agcgcgggca cgaagtacac
    22801 gtcgtcgttg gagtcaaggc tcgctgcgag ctcttcggtc tcctcggcgc tctcgatgat
    22861 gccgagcccg tccctgagcc actgcacggc tgcgccggtg atgaagattg cgccctccag
    22921 cgcgtactct acaggttcat caccgagacc ccacgcaatg gtggtgagca gctggcgctc
    22981 actctttatg ggctccgtgc ccgtcatcat gagggcgaag gagcctgtgc cgtaagtatt
    23041 ctttgtcagg cccttgtcga aggcgacctc gccgaagagg gcggcctgct ggtcgcccgc
    23101 aacgcctgct accggcacct cggccccgaa aacgtcggcg tcggtatcac cgtagacgta
    23161 ggaactcggc cttacctcgg ggagcgccgc acgcggcacg ccgcccagca tctccagaag
    23221 ctcgtcgtcc cactgcaaat cgaagatgtt gtacatcagg gtgcgtgaag cattggagta
    23281 gtcggtgacg tgggcgcggc caccggtaag cttgtagatc acccatgtat ctatggtgcc
    23341 gaatagaacc tcgccgttct gcgcccgctc cttgaggcca tccacgttct ccaggagcca
    23401 cctcaccttg gacccagaga aataggcgtc tatgacgagt cctgtcttgt tggcgaccat
    23461 ctcgtcgtag ccctgttcgg agaggtcgga gcagatctga gccccccgcc tgtcctgcca
    23521 cacgatggcg ttgtggacag gctcgccggt ctccttgtcc cacatgacga cggtctcgcg
    23581 ctggttggta atgccgatgg ccgcgatatc cccgggtgac gccccggcgt cctctagcgc
    23641 ctcccgcatc acccccaggg ttacgtccca gatctcattg gcatcatgct cgacccatcc
    23701 gggtctcgga taatgctggg tgaactcccg ctgggcctcc cccaccgtgg cgctgtcgtg
    23761 gttgaagact atcgccctgc tcgacgttgt cccctggtct atcgccagta cataatctcc
    23821 cgccatcacg cattctcctt ccgatacaga ccccgaacag tcagacgagc aatatctccc
    23881 ctacaacacg ccccacctcc taaactggct ttccccaagc acgcgcggcc gagacctgcc
    23941 tcaccccgac catgcacctt tactagcttt ctctatttcg agcgtaacgg agcccaacac
    24001 ccgagtcaag gatctctcgt acagggagat atagatccct gcccctcgcc tcagcgcccg
    24061 cgagaagatt cccgcccgat ccgtgagatc acgcagggaa gaaggtggca ggccgagagc
    24121 gtttacgagg ggtgatccga cctatccgcg cagggcgggg gtatactcag ccgcgatgga
    24181 cgagcggacg cgcataaggc tgac
//
LOCUS       DATMVF010000084         6248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_106349, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000084 DATMVF010000000
VERSION     DATMVF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106349"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VN178_03760"
     CDS             <1..200
                     /locus_tag="VN178_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80117.1"
                     /translation="ALEQHERNTDQSPEAFFEGMEFYNIVLDPEDGERVWEYRLLEDT
                     LLRARWLLRMLQQESEASNQR"
     gene            complement(697..1875)
                     /locus_tag="VN178_03765"
                     /pseudo
     CDS             complement(697..1875)
                     /locus_tag="VN178_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
     gene            complement(1878..3035)
                     /locus_tag="VN178_03770"
     CDS             complement(1878..3035)
                     /locus_tag="VN178_03770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS80118.1"
                     /translation="MGSPDQYEDLRRGVREVCADFPDAYWRELDARREYPEAFIEAMT
                     KSGYLGALIPEEYGGLGLSLTDATIILEEINRSGGNAQPAHAQVYVMGTMLRHGSEQQ
                     KREYLPKIASGQLRLQAFGVTEPEAGTDTTSLRTSAVRDGDQYVINGRKIYISRVQHS
                     DLMILLARTTPLDRVTRKSDGLSVFLIDLREAAGNGLTVRPRRVMINNETNELLFENL
                     SIPASSLIGEEGRGFRYILDGMNAERILIAAECIGNGRWFVDKATKRAGERVVFGRPI
                     GQNQGIQFPLARAHISVEAADLMRYRAAELFERGEPCGAEANMALFLTADASWEAANA
                     AMQTFGGYSYDAEYDVERKFRETRLFQVAPISTNLILSYVGEHVLGLPRSF"
     gene            complement(3037..4410)
                     /locus_tag="VN178_03775"
     CDS             complement(3037..4410)
                     /locus_tag="VN178_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWS80119.1"
                     /translation="MSVTRTATTRELASFLSGLRFEDLPDEVVERTKELFLDWAASAL
                     AGKNARPIRILERFTETMAPAEGPSEILVSRRRTSPLFAALVNGAASHVVEQDDLHNA
                     SVFHPATVVFPAVLAAAQQTGASGRDLISASVAGYETGVRVGSYLGRSHYKVFHTTGT
                     AGTLAAAAAVSHLLGADEEVMLHALGSAGTQAAGLWEFLRDAADSKQLHTAKAASDGL
                     LAAYLASDGFTGATRILEGDQGLAVGMSSDADPPKLVEGLGEKWAVPETSFKFHASCR
                     HTHPAADALLEAMGKHGLGADQIAGVRARVHGAAIDVLGPVSDPRTIHQSKFSMGFVL
                     AMIATAGSAGIDDFTEEALEDPALRGFSERVEMIFDPEIDAAYPQRWIGLVEIETTGG
                     EHITSRVDVPKGDPGNTLSREELEEKARNLAAFKGGASDAEMDRIIGRVWSLDLEPNV
                     RNLLPEA"
     gene            complement(4407..4913)
                     /locus_tag="VN178_03780"
     CDS             complement(4407..4913)
                     /locus_tag="VN178_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWS80120.1"
                     /translation="MAVKEGWRGRFFEDFKVGDVYEHPLGRTVTTTDNIWFTLLSQNT
                     APVHFDHNYAAQTEFGKPLVDSTFTLALVTGQSVTDISQNVFANLGWDEVRLPAPVIE
                     GDTIYSQSEVLEKRESRSRPNVGIVTVETPGYNQDGRTVITFKRTIMVYRRGHAPKIT
                     RITPGGDG"
     gene            complement(4927..>6248)
                     /locus_tag="VN178_03785"
     CDS             complement(4927..>6248)
                     /locus_tag="VN178_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWS80121.1"
                     /translation="TDRGTICNMITELGATAAVFPSDAQTNEWLAAQRREEDYIPLAA
                     DPDASYDENEVIELPQLEPLIAEPSSPGNVVPVREVAGTETVQVCVGSSVNSSYEDLA
                     TAAAVLRENIVHPRIEMTVTPGSRQILDTISKSGVYQDLVAAGARMLEPVCGPCIGVG
                     QAPSAGVPSVRTFNRNFPGRSGTSGDEVYLCSPATAAATALKGEISDPRELGEPPTIA
                     PAPSDPNMDDRQILNPPPPEEARNVEIVRGPNIVPPPEGRPLPEELEGHIVIVVEDDV
                     STGDMAPDGALGMSLWSNIPECAKYMFRRQDPEFHDRALEWGGGFIVGGHNYGQGSSR
                     EHAALAPLHLGIRAVIARSFARIHRRNLISQSILPLLFREEDDYERVSRGDTWKIEGV
                     REPIAAGEQTLVVNTDDGTKIELEIRLSPREREVLMAGGTLKFLRGA"
BASE COUNT         1039 a         1975 c         2024 g         1210 t
ORIGIN      
        1 aagcgttgga gcagcacgaa agaaacacgg atcagtcacc cgaagccttt ttcgagggca
       61 tggagtttta caacattgtg ctggatccag aggatgggga gagggtgtgg gagtaccgct
      121 tgctggagga cacgcttctt agggcgcgct ggctgttaag aatgctccaa caggaaagcg
      181 aagctagcaa ccagagatga tggtgtacct ggtgatggcc gcctgacggc cttcgcatgg
      241 gcttgttgtg tgatagccgg atgacgctac ggaataccat gacgccgtac cgtcaacgct
      301 tctataatta aggtgtcaag ggaccgggcg gacttagtcg ccgggactgg tccagttcac
      361 ccgtgaagca caacacgact taactgacac tagtttgaca ctaaccgggt gctacgacac
      421 gaaaaatcgt gcgacatcga ttgaaagcgt tagccttaaa caagcggatt ctgcgacacc
      481 taggaacacg ttgaaacaag tcgcctcgct cgactcatcc gccggccgca ggttcgagtc
      541 ctgccgagcg cgctcctttc cctgcaaatg gcaggcacct ctcggcatat ccaataccga
      601 ctggtgcgct gcgtgcggcg catgtcggtc cggtttgcag cgcagctgca gtaatttagc
      661 ttctaaggac ctgtcaggac aagacctggt ggctgctcat agggccgatg actgccgtag
      721 ggtcgtcacg gcgtcatcgt cgtagccgag ctcggcgagt atgctggcgg tgtgctctcc
      781 gacggaaggg atcggggcca tgacgggctc aatgccctct aaggtcgccg gcgggatgag
      841 ggcgcgcaga gggccgaccg gggatcccac ctccctccat cggtcccggg cttcgagctg
      901 gggatggtcg aggaagcctc ggacgttcct catacgggcg ttggctatct ttgccttctc
      961 gaggcgttct atggcttctt cggaggagag ctgcgcgagg atgccttcga tcgcctggcg
     1021 cagcgcgtcc acgttttcga cgcgctccga gttgctttcg aagcgttcat cgtcagccag
     1081 ggcaggctgt tctagcacga cctcgcagaa cctctcccat tcccgctcgt tctggaggcc
     1141 taggaagatt atctccccgt cgccacactc gaaaggaccg tagggggcga tagcggcgtg
     1201 gctcgccccg gttctcggcg gttccttgcc gccgtacaaa gtgtagtagg ctgggaagcc
     1261 catccactcg gcgagcgcct cgaagagcga aatttcgagg gtggcgccct caccggtccg
     1321 ctcgcgccgg aacagcgcgg ctagtatgcc cgagtaggcg tacatgccgc aggctatgtc
     1381 cgcgatggaa acccctacct ttgagggagc ttcgggcgta ccagtgatgg atacgagtcc
     1441 ggcctcgcac tggacgagca agtcgtaggc cttcttgtcc ctgtacggac ccgaggcgcc
     1501 gtagcccgac acgtcgcaca cgacgagcct gggatacctc tgccgaagag cctccgcgcc
     1561 gaagccgagc ctgccggccg cgcccggcgc caggttctgt ataaagacgt cggccttctc
     1621 gatgagccta ttcagtatct ccttcgcccc ttcctccttg aggttcagcg tcaaagactc
     1681 tttggagcgg ttgagctaca cgaagtgact ggagagaccc ttcactgttc tgtcgtaacc
     1741 gcgggcgaag tccccaacct ccggacgctc gacctttatc actcgggcgc ctaggtcggc
     1801 gagctggcgc gtggcgaacg gcgcggcgac cgcctgctcc aaagatacga ccgtgatatc
     1861 ttctagcggc agcacgacta gaaggacctc ggcaatccga gcacatgctc gccgacgtag
     1921 gagaggatga ggttcgtgga gatcggggcc acttggaaga ggcgggtctc gcggaacttg
     1981 cgttccacgt cgtactcggc gtcgtaggag taaccgccga aggtctgcat cgccgcgttg
     2041 gcagcttccc aagaggcgtc ggcggtcaag aaaagggcca tgttcgcctc ggcgccgcag
     2101 ggctcgcccc tttcgaaaag ctccgcggcc ctgtagcgca tcaggtcggc ggcctctacg
     2161 cttatgtgcg ctcgggcgag ggggaactgg atgccctggt tctggccgat cggacgcccg
     2221 aagaccacgc gttctccggc cctcttggtc gccttgtcga caaaccaccg cccgttacct
     2281 atgcactcgg cggctatgag gatccgctcc gcgttcatgc cgtcaaggat gtaacggaag
     2341 cctctgcctt cctcgccgat caggctgctc gccgggatgc ttaggttctc gaagaggagc
     2401 tcgttggtct cgttgttgat catcacgcgt ctgggcctca ctgttagacc gttgcccgcg
     2461 gcttcgcgca ggtcgatgag gaatacggag agcccatcag acttcctcgt cacccgatcc
     2521 agcggcgttg tacgggcaag caggatcatg aggtcggagt gctggacgcg cgagatgtag
     2581 atcttgcgcc cgttgataac gtattgatcg ccgtcgcgca cggcgctcgt cctcaaggag
     2641 gtcgtgtcgg tcccggcctc cggctcggtc acgccgaagg cttgcagcct cagctgaccg
     2701 ctggctatct tcggcaagta ctccctcttc tgctgctccg agccgtgacg cagcatagtg
     2761 cccataacgt acacctgcgc gtgggcgggc tgggcgttgc ccccggaacg attgatctct
     2821 tcgaggatga tcgtagcgtc ggtgaggctc aagcccaacc cgccgtattc ctccgggatg
     2881 agcgcgccga ggtagccact cttcgtcatc gcctcgataa aggcttccgg gtactcccgt
     2941 ctcgcgtcga gctcgcgcca gtaggcgtct gggaagtccg cgcacacctc gcggactcct
     3001 cgccgcaagt cctcgtattg gtcggggctc cccacgttac gcctccggca gcaggtttcg
     3061 cacgttgggc tccagatcta ggctccagac gcgaccgatg atacggtcca tctctgcgtc
     3121 cgaggccccg cccttaaagg cggcgaggtt ccgggctttc tcctccagct cctcgcggct
     3181 caacgtgttc ccggggtccc ccttcgggac gtctacgcgg gaggtgatgt gttcgccgcc
     3241 ggtcgtctct atctccacga gcccgatcca acgctgcgga taggccgcgt ctatctcggg
     3301 gtcgaagatc atctcgaccc gctcgctgaa cccgcgcagc gccgggtcct cgagcgcctc
     3361 ctcggtgaag tcgtcgattc cggcgctccc cgcggtggcg atcatggcga gtacgaaccc
     3421 cattgagaac ttggactggt ggatcgtgcg cgggtcgctc acgggtccga gcacgtctat
     3481 cgcagcgccg tgcacgcggg cgcggacgcc ggctatctga tccgcgccaa ggccatgttt
     3541 tcccatcgcc tcaagcagtg cgtcggcggc ggggtgcgtg tgccggcagg aggcgtggaa
     3601 cttgaacgag gtctccggca ctgcccactt ctccccgagc ccctcgacca acttcggggg
     3661 gtccgcgtcc gaggacatcc cgacggccag cccctgatct ccctccagga tgcgggtcgc
     3721 gccggtgaac ccgtcgctgg cgaggtaggc ggcgagaaga ccgtcggatg cggcctttgc
     3781 ggtgtggagc tgcttggagt ccgccgcgtc gcgcaggaac tcccacaacc cggcggcttg
     3841 cgtcccggcc gaaccgagcg catggagcat gacctcttcg tcggcgccca aaaggtggga
     3901 gacggcggcg gccgcggcga gcgtgcccgc cgtcccggtc gtgtggaaga ccttgtagtg
     3961 ggagcggcct agatagcttc cgaccctcac cccggtctcg tagccggcca ccgaggcgga
     4021 gatcaggtct cgtcctgagg ccccggtctg ctgggctgcg gcgaggacgg cagggaagac
     4081 cacggtcgcc gggtggaaaa ctgaggcgtt gtggagatcg tcctgctcga caacgtgcga
     4141 cgcggcgccg ttcacgagcg cggcgaagag cggcgaggtg cgccggcgcg agaccaggat
     4201 ctcgctcggc ccttcggctg gagccatggt ctcggtgaat cgttcgagga tgcggatcgg
     4261 ccgcgcgttc ttgccggcga gggccgatgc ggcccaatcg agaaacagtt ccttggtgcg
     4321 ctcgacgact tcatctggca ggtcctcaaa gcgtaatccg gagaggaaag aagccagctc
     4381 gcgggtcgtc gcggtcctgg ttacgctcat ccgtcacctc cgggagtgat cctggtgatc
     4441 ttcggggcgt gaccccgcct gtagaccatg atggtgcgct tgaacgtgat gaccgtcctc
     4501 ccgtcctggt tgtacccggg cgtctcgacc gtcactatcc ccacgttcgg gcgcgagcgc
     4561 gactcgcgct tctccaggac ttccgactga gagtagatcg tgtccccctc gatgacgggc
     4621 gccggcagcc tcacctcgtc ccatcccagg ttggcgaaca cgttctggga gatgtccgtc
     4681 acgctctgcc ccgtgaccag cgcgagggtg aaggtagagt ccacgagcgg cttgccgaac
     4741 tccgtctgtg cggcgtagtt gtggtcgaag tggacggggg cggtgttctg ggagagcagg
     4801 gtgaaccaga tgttgtccgt ggtggttacc gtacgtccta aagggtgctc gtagacgtcg
     4861 ccaaccttga agtcctcgaa gaaccgtccc ctccagccct ctttcaccgc catgcgactc
     4921 cctttcctat gcgccacgca agaacttcag agtcccgccg gccatgagga cctcgcgctc
     4981 tcgaggcgac agccggatct cgagctcgat cttcgtcccg tcgtcagtgt ttaccacgag
     5041 tgtttgctct cccgcggcga tcggttcacg cacgccttcg atcttccagg tatcgccccg
     5101 gctcacccgt tcgtagtcgt cctcttcacg aaacagcagc ggaaggatgc tctgggagat
     5161 caggttccga cggtggatgc gcgcgaagct cctggcgatg acagcgcgga ttcccaggtg
     5221 cagcggagcc aaagcagcgt gttcgcggct ggagccctgc ccgtagttgt gcccgccgac
     5281 gataaaaccg cccccccact cgagcgcgcg gtcgtggaac tctgggtcct gccggcggaa
     5341 catgtacttc gcacactcgg gtatgttcga ccagagactc atcccgagcg ccccgtcggg
     5401 agccatgtct ccggtcgaga cgtcgtcttc gacgactatg acgatgtgtc cctccagctc
     5461 ttctggaagc ggtcggccct caggcggggg cacgatgtta ggtccgcgga cgatctcgac
     5521 gtttcgcgcc tcctcgggtg gcggcgggtt caggatctgg cggtcgtcca tgttcgggtc
     5581 ggacggtgcg ggggcaatgg tcggcggctc ccccagctcg cgcgggtcgg agatctcgcc
     5641 cttgagcgca gtcgcggcgg ccgtcgccgg ggagcaaagg tagacttcat ctccgctcgt
     5701 cccgctgcgg cccgggaagt tgcggttgaa ggtcctcacc gagggaacgc cggcggatgg
     5761 ggcctgtcct acgccgatgc agggaccgca gacgggctcg agcatgcgcg cgccagcggc
     5821 gacgaggtct tggtagacgc cgctcttcga aatggtgtcc aggatctggc gcgaccccgg
     5881 cgtgaccgtc atctctatgc gggggtgcac gatgttctcc cgcaggacgg ccgcggcggt
     5941 ggcgaggtct tcgtaggagg agttcaccga ggagccgacg cacacctgga ccgtctccgt
     6001 gccggcgacc tcgcgcaccg gtaccacgtt gcccggagag gacggttccg cgatcagcgg
     6061 ttcgagctgc gggagctcga tcacttcgtt ttcgtcgtaa gaggcatcgg ggtctgcggc
     6121 gagcgggatg tagtcctcct cacgcctctg ggcggcgagc cactcgttcg tctgcgcgtc
     6181 gctcgggaag acggcagcgg tggctccaag ctccgtgatc atgttgcaga tcgtgccccg
     6241 atccgtca
//
LOCUS       DATMVF010000085         4673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_152039, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000085 DATMVF010000000
VERSION     DATMVF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152039"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /gene="ricT"
                     /locus_tag="VN178_03790"
     CDS             <1..739
                     /gene="ricT"
                     /locus_tag="VN178_03790"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HWS80122.1"
                     /translation="LTPRRREPYTQPYDIVRIVTEEDARTEDRNRELGRGVREEARKL
                     ARDHTIEGVQFIGCDVSLDGSYIEVKYQTDEKELDLEPVKSGLERAYDAQVAFREFRF
                     IERSGDAGGCDTCGLPLCCATWSGARSMGPVNVRLAKQQGVTPNEKILGCCGEVKCCM
                     RYEHDTYKEFKQRAPFRNSTVKIGDREGKVVDYSMVKDSVFVQFGSKRADQELLSLGS
                     LARDNPGIMPADTDEWELPEPPSGKDS"
     gene            747..1709
                     /locus_tag="VN178_03795"
     CDS             747..1709
                     /locus_tag="VN178_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWS80123.1"
                     /translation="MLDNLCDVPGVLVGHATHREGHTGCTAVIFEGQDGAVVGVDVRG
                     SSPGTRETDRLGPVGAVRPTHALLLTGGSAFGLAAVDGVVRFLEEIDVGLDMGVARIP
                     LVSAAVLFDVVVGDPYVRPDAAMGYEAASAATSGDFAQGSVGAGTGATVGKVLGMDRA
                     MKGGLGSASVHLAGGLIVGAIAAVNAFGEVRDPQTRKILAGPRLDDGTLGDTVELLPE
                     AAARLRWGENTTLGIVATNARLSKPQVSKVAQMGHDGLARTIYPVHTSVDGDVVFAAS
                     VGMVEARPDVVGAWGARVLEKAIVRAVLSAESIAGLPAAKDVTR"
     gene            complement(1726..2793)
                     /locus_tag="VN178_03800"
     CDS             complement(1726..2793)
                     /locus_tag="VN178_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWS80124.1"
                     /translation="MKAARLHRYDESIPKDSLKVEEIDEPRIENPLEVIVRVGAAGLC
                     RTDIHVVEGQWQPIQDADNTLLPYVLGHENSGWVEEVGSAVTNVEVGDTVICHPLMTC
                     GLCRACRAGDDMHCENGEFPGISRDGGFADLLKTNARALVKLDPILEPKDIAALADAG
                     LTAYHAVRKSVPHLYAGTKAVVIGAGGLGHIGIQCLKALTPTEIIVTDPSEEALGLAR
                     EWGADQTVKVEDGYVDTVLEMTEGMGAEVVFDYVGERGAQDDAWMMTRPGGSHYVIGY
                     GGMVEVPAIDIISTERNITGNLVGTYNDLAELMVLTAQGKVNLHTSTYPLDAVNDAIQ
                     DLNNGELRGRGILIPESANAA"
     gene            complement(2790..3497)
                     /locus_tag="VN178_03805"
     CDS             complement(2790..3497)
                     /locus_tag="VN178_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HWS80125.1"
                     /translation="MTVDEARILGALSGVRDPELDEPITELGFVSNLEIEGEAVNIRL
                     RLPTYFCAPNFAYLMVADAREAALSVPGIGKAKVILEEHYASEELNSGVNAGGGFDDA
                     FPGETEGPDLEELRTIFRRKSFVSRQERLCRTLLSEGRSPQELAAMRLGEMATSQDFE
                     KYLERRKELGLDVSEESPLVVDPDGNKVPEEAVIEHLRFAKTVRVSIEGNAGLCRGLL
                     DVRYGGRRGVGQKEAML"
     gene            complement(3494..4534)
                     /locus_tag="VN178_03810"
     CDS             complement(3494..4534)
                     /locus_tag="VN178_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWS80126.1"
                     /translation="MYEKDGEKYFVVDGHVHYWDASPENRNKYGEGFINCFYDYHKNL
                     SPEEWVWPKEKYDKYTEEDMMHDLFETGYVDVAIFQPTYLTEFFPHGFNTTEQNAVLK
                     EKYPDKFILNGSFDPREGVQGLEQFEELVDKYDLKGVKLYTAEWKGDSRGWKLSDPSA
                     TKYLEKCVELGVKNIHVHKGPTIWPLNRDAFDVADVDEAASEFPDLNFIVEHVGLPRL
                     EDFCWIGVQEPNVYGGLAVAIPFIHTRRHYFTQVIGELLYWLGEDRLLFGSDYAIWSP
                     KWLVEEFVDLQMPESGEFEDFSELTPEVKRKILGLNAAKLYDIEVPEELQVEHAGTGP
                     AVTEKPAADLPT"
BASE COUNT          836 a         1503 c         1411 g          923 t
ORIGIN      
        1 cctgacgcct cgccgccgcg agccttacac ccaaccctac gacatagtca ggatcgtcac
       61 cgaggaggac gccaggacgg aggatcgcaa cagggaactg ggacgcggcg tcagggaaga
      121 ggccagaaag ctcgccaggg accacaccat cgagggcgtg cagtttatcg gctgcgacgt
      181 ctccctggac ggctcctaca tcgaggtcaa ataccagacc gacgaaaagg aactggatct
      241 ggagccggtc aagagcggcc tcgaacgggc ctacgacgcg caggtcgcct tcagggagtt
      301 ccgctttata gagcggtcgg gggacgccgg cggatgcgac acgtgcggcc tgcccctgtg
      361 ctgtgcgacg tggagcggtg ccaggagcat gggtcccgtc aacgtcaggc tggccaagca
      421 gcagggcgtg acgccgaacg agaagatcct gggctgttgc ggggaggtga agtgttgcat
      481 gcgctacgag cacgacacct acaaggagtt caagcagcgc gcgcccttcc gcaactcgac
      541 cgtgaagatc ggtgacaggg aaggcaaggt cgtcgactac tccatggtca aggattccgt
      601 cttcgtccag ttcggatcca agcgcgcgga tcaagaactc ctctccctcg gctccctggc
      661 cagggataat cccggcatca tgcccgccga cacagacgag tgggaattgc cggaaccccc
      721 ctcgggcaag gactcttaac accggcgtgc tggataacct gtgcgacgta ccaggtgtgc
      781 tcgttggcca cgccacacac agagaagggc atacgggctg caccgccgtt atcttcgagg
      841 gccaggatgg tgctgtcgtc ggcgtggacg tacgtggctc ctcccccggt acgcgcgaga
      901 ccgaccgcct gggaccggtc ggcgccgtcc ggcccaccca cgccctgctc ctgaccggcg
      961 gtagcgcctt cgggctggcc gccgtggacg gcgtcgtccg cttcctcgaa gagatagacg
     1021 tcggcctcga catgggcgtg gcccgcatac cgctggtgtc ggcggccgtt ctcttcgacg
     1081 tcgtcgtagg agatccttat gtacgtcccg acgcggcgat gggctacgag gcggcctcag
     1141 cggccacgag cggcgacttc gcccagggga gcgtgggagc agggacgggt gcgacggtgg
     1201 ggaaggtcct cggcatggat cgcgcgatga aagggggcct ggggagcgca tcggtccacc
     1261 tcgctggtgg ccttatcgta ggcgccatcg ccgccgtgaa cgccttcggt gaggtcaggg
     1321 acccgcaaac caggaagata ctggcgggtc cgcgactgga tgacggaacg cttggcgata
     1381 cggtggaact gctccccgag gccgctgcgc gcttgcgctg gggagagaac accacgctcg
     1441 ggatcgttgc caccaacgcc cgtctctcca agccacaggt tagcaaggtc gcccagatgg
     1501 gccacgacgg ccttgcccgc actatatacc cggttcacac gagcgtggac ggcgacgtcg
     1561 tcttcgccgc ttcggttggt atggtggagg cgaggcccga cgtcgtcggc gcgtgggggg
     1621 cgcgggtcct cgagaaggcc atcgtgcgcg ccgtcctctc cgcagaaagc atcgccggcc
     1681 tgcctgcggc gaaggacgtt acgcgctaac cccgcacttc aataatcagg ctgcgttcgc
     1741 gctttcgggg atcagtattc cccttcccct gagctcgccg ttgttgaggt cctgtatcgc
     1801 gtcattgacg gcgtccagcg ggtaggtcga cgtgtgcaag ttgaccttgc cctgggcggt
     1861 cagcaccatg agttcggcca ggtcgttgta ggtgccgacc aggttgccgg tgatgttgcg
     1921 ctcggtcgag atgatgtcta ttgcggggac ctccaccatg ccgccgtagc cgatgacgta
     1981 gtgggagcca ccgggtcggg tcatcatcca ggcgtcgtcc tgcgcgccgc gctcgcccac
     2041 gtaatcgaag accacctcgg cgcccatgcc ctcggtcatc tccagaaccg tatcgacgta
     2101 accatcttcc accttcaccg tctggtccgc tccccactcc ctggcgagcc cgagcgcctc
     2161 ctccgaaggg tcggttacga tgatctccgt cggggtgagc gccttgaggc actggatgcc
     2221 gatgtgcccg agtcccccgg ccccgatgac cacggcctta gtgcccgcgt agaggtgggg
     2281 caccgacttc ctgaccgcgt ggtaagcggt gaggccagcg tcggcaaggg cagctatgtc
     2341 cttgggctcc agaatgggat ccagcttcac cagtgcacgg gcgttggttt tcaagagatc
     2401 ggcgaagccg ccgtcgcggg agatgccggg gaactcgccg ttctcgcagt gcatgtcgtc
     2461 cccggcgcgg cacgcccggc acaggccgca ggtcatgagc gggtggcaga tcaccgtgtc
     2521 cccgacctcg acgttggtca ccgcggagcc gacctcctcg acccatcctg aattttcgtg
     2581 ccccaggaca tatggcagca gcgtgttgtc cgcgtcctgt atcggttgcc actggccctc
     2641 gacgacgtgt atatccgtgc ggcagagccc ggcggccccg acgcgtacga tcacctcgag
     2701 cgggttctct attctgggct cgtctatctc ctcgaccttg aggctgtctt tgggaatgct
     2761 ctcgtcgtag cggtgcaacc gcgcagcctt catagcatag cctccttctg tcccaccccg
     2821 cgcctgccac catatcgtac gtctagcagc ccccggcaca gccctgcgtt gccctcgatg
     2881 ctcaccctga cggtcttcgc gaacctgagg tgctcgatga cggcctcctc aggcacctta
     2941 ttaccatccg gatccacaac cagcggtgac tcctccgaga cgtcgagccc gagctccttg
     3001 cggcgctcca agtacttctc gaaatcctgc gatgtcgcca tctccccgag gcgcatcgcg
     3061 gcgagctcct gaggagagcg cccctccgag agcagcgtcc ggcatagtcg ttcctgccgc
     3121 gaaacgaacg acttgcggcg gaagatagtt cgcaactctt cgaggtccgg accttctgtc
     3181 tcacctggga aagcgtcgtc gaatccacct ccggcattga cgcccgagtt taattcctct
     3241 gaggcgtagt gctcttcgag gattactttg gccttaccta tgccgggtac cgagagcgcc
     3301 gcttctcttg cgtcagcgac catcaggtag gcgaagttcg gagcgcagaa gtaggttggc
     3361 aaccgcagcc gaatatttac tgcttcgccc tctatctcga gattcgagac gaagcctagc
     3421 tccgttatgg gctcatccag ctcggggtcc cggacgcccg aaagggcgcc cagaatcctt
     3481 gcctcgtcta ccgtcatgtg ggcaggtcgg cggcaggttt ctctgtaacc gcggggcccg
     3541 tgcccgcatg ctcgacctgc agctcctcgg gcacttcgat gtcgtagagc tttgcggcgt
     3601 tgagacccag tatcttgcgc ttgacctccg gagtgagctc cgagaagtcc tcaaactcgc
     3661 cagattcggg catctgtaag tccacgaact cctcgacgag ccacttcggc gaccatatcg
     3721 cgtagtcgct gccgaagagc agcctatctt ctcccagcca gtacagtagt tcgccgatga
     3781 cctgagtgaa gtaatgccgg cgcgtatgaa tgaacggtat tgctaccgcc agtccaccgt
     3841 agacgttggg ctcctgcacc ccgatccagc agaaatcctc gagcctcggc aacccgacgt
     3901 gctctacgat gaaattgaga tccgggaatt ctgaagccgc ctcatccacg tctgcgacgt
     3961 cgaacgcgtc cctgttcaaa ggccagatgg ttggtccctt gtgcacgtgg atgttcttga
     4021 ccccgagctc cacgcatttc tccaggtact tggtagcgga gggatcagag agcttccagc
     4081 ccctggagtc tcccttccac tcggctgtat agagtttgac gcccttgagg tcgtatttgt
     4141 ctaccagctc ctcaaactgc tcgagtccct gcacgccctc gcgcgggtcg aaggagccgt
     4201 tgaggatgaa cttgtccggg tacttctcct tcaagaccgc gttctgttca gtagtgttga
     4261 agccatgggg gaagaattct gtcaggtaag taggctggaa gatcgcaacg tctacgtagc
     4321 ccgtctcgaa gaggtcgtgc atcatatcct cttcggtgta cttgtcgtac ttctccttcg
     4381 gccagaccca ttcctcggga ctcaggttct tgtgatagtc gtagaagcag ttgatgaacc
     4441 cctcgccgta cttgttgcgg ttctcggggc tggcatccca ataatggacg tgcccgtcaa
     4501 ccacgaaata cttctctcca tccttctcgt acaagtgatc tcctctccga aacaaacgac
     4561 aatgccgccc cactcgaacc cgccgtatca cccctttctt ccggcttgcc ttacccctca
     4621 aggtccaata tatccggctg tgtccgtcaa tcaaggcaca aatgcgtagc aag
//
LOCUS       DATMVF010000086         5593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_335015, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000086 DATMVF010000000
VERSION     DATMVF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335015"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VN178_03815"
     CDS             complement(<1..625)
                     /locus_tag="VN178_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS80127.1"
                     /translation="MDGRKDRATTLVEASLLLAVFFLGTNPVAVKVAVAEFPPVPFVV
                     IRFTLAGLLLLALVAFLEPREGRPGRRDLLSLAGVGLVGVGANNVAFTLGVSMTTASE
                     TALIYAAVPIWGILLGLALGLERPTPWGILGVCLAFLGVGVVVYGGLTGSTSLLGNLL
                     VVVATVCWGSYAVLSLPLLRRYSPLVVASYTMLFGGLGALPLALPGFL"
     gene            complement(735..3734)
                     /locus_tag="VN178_03820"
     CDS             complement(735..3734)
                     /locus_tag="VN178_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWS80128.1"
                     /translation="MRDVTRRTETEERLRSQSRELALLHRVRTAVARELEVPGVLARA
                     VEAVAETYGYTRLSAYLLEGKELVLQHSAGYHGALGRIPLTKGVRGRVVRSGRPLLVE
                     DVSADPDSLGPIDGVTSEICVPLFDEGEAVGLLDLRSVNGVKLTENDLRVMVAVTELV
                     DLAIGRARLYARVRYSEERYRALTQNSSDLVTVMETTGIVRYQSPAIERMLGYSSEEL
                     LGRNAFDYVHPDDLQRVKLVYDEGLKDPGLHPSTEYRFRHKDGSWRWLESVGTNLIQE
                     PGVGGYVVNSRDITRRKEAEDRLREAEKRYRMLVERIPAITYIQEIRRNNEIVYVSPQ
                     VMDIMGYTPEECTTTPDFWIRILHPDDREAVLAEDQRTNETGEPFVMEYRRFAKDGRL
                     VWIRDEATLVRNEAGEPLYWLGVQIDITERKRTETRLGDAQQRFRTLVDQIPAVTYID
                     PVDDPETSLYTSPQIEKMLGYTPEEWREGKLWPKCLHPDDRERILAADERFEAGGEES
                     FSEEYRLMARDGSVVWVREEAVLLRDEAGEPLYWQGVIYDLTERKALEERLEHRAFHD
                     YLTDLPNRQLFVDRLGKALDRTRRKSGRNVAVLFMDLDEFKIINDSLGHEAGDALLTL
                     VAQRLTRCLRPEDSLARFGGDEFVVLIEDIEGPEVAVRVAKRITEELKRPFFVEGREL
                     FASSSIGIGLGDASAKTPEALLMDADTAMYRAKEEGRPFRVFDPDMYRRAKGRLELEA
                     DLRRTLEAPYERLPVFYQPMVSIPDGTIVGMEALVRWAHPEQGLLVPPGFIPMAEETG
                     LIVPMGRWVLDESCRRAREWQERYPKAAPLTMAVNISARQLRYPELVGEVEDALRKAG
                     LDPGSLTLEITESVLVEDEGSSAGTLQRLKELGVRLAIDDFGVGYSSLSYLRYLPVDQ
                     LKLDRVLVGDLDTDDKNLAIVRAAVDLGHALGIEVVAEGVETHEEFERLSKLGCDIGQ
                     GDYWWGPQLRRETEELLASNHTPLADQ"
     gene            complement(4052..4822)
                     /locus_tag="VN178_03825"
     CDS             complement(4052..4822)
                     /locus_tag="VN178_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80129.1"
                     /translation="MRETPIQVAYPSVDNPNLRLALGACRFTARPGEGDEWVAGTYSD
                     PTDRRSMRIVEEETGVTITESEPSFQQIPAVFGGVPRYEVEFGKQRPFILTIETGASD
                     FDLDLGGVPLSRLMVRLGAGKFELGFSHPNPEPMELLEISSGAAAIEVENLANANFSE
                     MRLSGGAASYELDFGGVLSRDAEVSIETGISGVVVSVPSSTATRVVAETTLGSVDVGD
                     GFTKSEGAFSTGAALSGVGPVLRIRAGVRLGSLQLTTT"
     gene            complement(4875..>5593)
                     /locus_tag="VN178_03830"
     CDS             complement(4875..>5593)
                     /locus_tag="VN178_03830"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HWS80130.1"
                     /translation="RMNRSLPMLVAAAAVAMLGGACQSQAVGEMQRDSQKVPPENAQS
                     VRAHLKIGAGELNVSGGADALMEAEFAYNVADWKPDVSYDVSGDTGELSVEQGSGQDV
                     RPGGDARNEWDVLFSDEIPTDLRVQMGAGESSLDLDSLTLKGLDLQMGAGKTTVDLTG
                     DYTGDFDARIQGGVGEATVMLPSEVGVRARAEGGLGNINAKGLTKEGDSYVNEAYGSS
                     DVTLEIDVQGGIGQINLEVV"
BASE COUNT          944 a         1871 c         1694 g         1084 t
ORIGIN      
        1 ccaagaagcc tggcagggcg agaggcaggg ctccaaggcc cccgaacagc atcgtgtacg
       61 acgccaccac gagcggcgag tacctccgca agagcggcag agacaggacg gcgtaggacc
      121 cccagcaaac cgtcgccacc acaaccagga ggttgcctag gaggctcgtg ctgcccgtca
      181 ggccaccgta gactacgact ccgacgccca ggaaagcgag acacacgccc aggatacccc
      241 agggggtcgg cctctccagc ccgagcgcga ggccgaggag gatgccccag atcggtaccg
      301 ccgcgtagat cagggcggtc tccgaagcgg tggtcatgct caccccgagc gtaaaggcca
      361 cgttgttcgc ccccactccg acgaggccta cgcccgccag ggacaggagg tctcgccgac
      421 cgggtctccc ttccctcggc tcgaggaacg ccacgagcgc gaggagcagg agcccggcca
      481 gcgtgaacct gatcaccacg aaggggacgg gagggaactc ggcgaccgcg actttgacgg
      541 ccacggggtt ggtgccgaga aagaagaccg cgaggagcag cgaggcctcc acaagcgtcg
      601 tcgccctgtc cttgcgcccg tccatgaccc cgagtctacc gtcctcgcaa cacggttacc
      661 ggtcagttgg tctctacttc gggcttgccg tctgtagcga ggtatatctc ctgaggcggg
      721 acgcgttatc ggggttattg gtcggccaag ggtgtgtggt tcgatgcaag aagctcttcc
      781 gtctccctac ggagttgtgg gccccaccag tagtcgccct gtccgatgtc gcatcccaac
      841 ttgctcagtc tctcgaactc ctcgtgggtc tctacgccct cggcgaccac ctcgatgccc
      901 aacgcgtgcc cgaggtctac cgccgcccgc acgatagcca ggttcttgtc gtccgtgtcc
      961 aggtctccga cgagtactcg gtccagcttc agttggtcga ccggcaggta cctcaggtac
     1021 gacagcgagg agtatcccac cccgaagtcg tctatggcca gccgcacccc aagttccttg
     1081 aggcgctgga gcgtgcccgc gctggatccc tcatcctcta ccaggacgct ctccgtgatc
     1141 tccagcgtaa ggcttccggg atcgaggccg gccttcctca aggcgtcctc gacttcgccg
     1201 actagttccg ggtaacgcag ttgacgggcg gagatgttga cggccatggt cagcggggcg
     1261 gccttggggt agcgttcctg ccactccctg gcgcgccggc aggactcgtc gagcacccac
     1321 cggcccatcg ggactatcag gcccgtctcc tcggccatgg gaatgaatcc aggagggacg
     1381 agcaaacctt gctccggatg ggcccacctc acgagcgcct ccatccccac gatggtgccg
     1441 tcgggtatgg agaccatcgg ctggtagaag acgggtaagc gctcataagg agcctcgaga
     1501 gtccgtctga ggtcagcctc cagttccagt cggcccttgg cccgtctgta catatccggg
     1561 tcgaataccc tgaaaggcct gccctcttcc ttggccctgt acatcgccgt gtcggcgtcc
     1621 atgagaagtg cttcgggcgt cttcgcactg gcgtcgccca ggccgatgcc tatgcttgaa
     1681 gaggcgaaca gttcacggcc ctccacaaag aacggtctct ttagttcctc ggtaatcctc
     1741 tttgctacac gcactgctac ctcggggccc tcgatgtcct cgatgagcac cacgaactcg
     1801 tcgcctccga agcgggccag gctatcttcg gggcgcaggc agcgcgtgag gcgctgggca
     1861 acgagcgtga gtagcgcatc cccggcctcg tggccgagcg agtcgtttat tatcttgaac
     1921 tcgtccaggt ccataaagag caccgccacg ttccggccac tcttgcgcct cgtgcgatcc
     1981 agggcctttc caaggcggtc cacgaagagc tggcggttcg ggaggtcggt caggtagtcg
     2041 tggaaggccc ggtgctcgag ccgctcctcc agcgccttgc gctcggtgag gtcgtagatg
     2101 accccttgcc agtagagagg ttcgcccgct tcatctctca ggaggaccgc ttcctcgcgg
     2161 acccacacca ccgatccgtc cctggccatc agacggtact cttcgctgaa ggattcctcg
     2221 ccccccgcct cgaagcgctc gtcggcggcc agtatcctct cccgatcgtc cgggtggagg
     2281 cacttcggcc acagcttacc ctccctccac tcctcggggg tgtatccgag catcttctct
     2341 atctgggggc tggtgtagag cgaggtctca gggtcgtcca ccgggtcgat gtaggtgacg
     2401 gcggggatct ggtcgaccag ggtacggaaa cgctgctgtg catccccgag ccgcgtctcg
     2461 gtccgcttgc gctccgttat gtctatctgt accccgagcc agtagagggg ttcccccgct
     2521 tcgttcctga cgagcgtcgc ctcgtccctg atccacacca ggcgcccatc cttggcgaat
     2581 cgacgatact ccatgacgaa aggctctccg gtctcgttgg tacgctgatc ctcggccagg
     2641 accgcctccc tgtcgtcggg atgaagaatc cttatccaga agtccggggt agtggtgcac
     2701 tcctcgggcg tgtaacccat gatgtccata acctgggggc tgacgtaaac gatctcgttg
     2761 ttgcggcgta tctcctggat gtaggttata gccggtatcc gctcgacgag catccggtag
     2821 cgcttctcgg cctccctcag gcggtcttcg gcctccttgc gcctcgtgat gtccctggag
     2881 ttgaccacgt atccgcccac gccgggttcc tggatcaggt tcgtgccgac cgactcgagc
     2941 cagcgccagg agccgtcctt atggcgaaag cgatactcgg tcgagggatg cagcccggga
     3001 tctttgagtc cctcgtcgta gaccagcttc accctttgca agtcgtcggg gtgcacgtag
     3061 tcgaaagcgt tcctcccgag gagttcctcc gacgagtagc cgagcatccg ctctattgcc
     3121 gggctttggt agcggactat gcctgtggtc tccatcaccg tcacgaggtc cgaagagttc
     3181 tgggtgaggg cacggtagcg ttcctcgctg tagcgcaccc tggcgtagag gcgggcccgg
     3241 ccgatcgcca ggtccacgag ctcggtcacg gcgaccatca ccctcaggtc gttctccgtc
     3301 agctttaccc cgttcacact cctcaagtcg aggaggccca cagcctcgcc ctcatcgaag
     3361 aggggaacgc agatctcgga ggtcacaccg tctatgggtc ccagggagtc ggggtcggcg
     3421 ctcacgtctt ccacgagcaa gggacgtccg gagcggacga cccgcccgcg gacccctttc
     3481 gtcaggggga tccgcccgag ggccccatgg tacccggcac tgtgctggag caccagttcc
     3541 ttgccttcga gcaggtaggc gctcagccgg gtgtagccgt aggtctcggc cacggcctct
     3601 accgccctgg cgagtacgcc aggcacctcc aactcacgcg ctactgccgt gcgaacccgg
     3661 tggaggaggg ccagttccct cgactggctg cgaagcctct cctcggtttc tgtacgcctc
     3721 gtaacgtccc tcaaagagat ctccacgccc tagagggcag ggtgccgccg aggcctacga
     3781 ccttcgtgat ctccgaggag ctctccacga ccgggcgcaa acgccggtcg tcttgccggg
     3841 aaagcctatt agtatgatcg ggcttgtctt cgccgcgggg gtcacggctc ggtaggctgt
     3901 tcggatccgg ctttggaggc tctaccaatg cagtctttcc ggccactgtc gatgactgga
     3961 ccggagtata cccgagatag atgctatctg gccacgggca ccgccgggcg aggaactcgg
     4021 gacaggggcg cagatcgtcg cggcgaagag ctcaagtcgt tgtgagctgc agcgacccca
     4081 gcctcactcc ggcgcggatt ctcaagacgg ggccaacccc gctaagggcc gctccggtcg
     4141 agaaggcgcc ctcgcttttc gtgaaaccgt cgcccacatc gacgctgccg agggtggtct
     4201 cggcgaccac ccgcgtcgcg gtagaggagg gaacggacac cactacccct gagattcccg
     4261 tctctatgct cacctcggcg tcccgcgaga gaacaccgcc gaagtccagt tcgtagctgg
     4321 ccgcccctcc tgagagacgc atctcggaga agttggcgtt ggcgaggttc tctacctcta
     4381 tggcggcggc cccgctggag atctccagca gttccatggg ctcggggttg gggtgcgaga
     4441 agccgagttc gaacttgccg gcgcccagcc tgaccatcaa acgactcaag ggtacgcccc
     4501 cgaggtccag atcgaagtcg ctggcaccgg tctcgatcgt caggatgaag ggtctttgct
     4561 tgccgaactc gacttcgtag cggggtacgc caccgaagac cgcagggatc tgttgaaagg
     4621 agggctccga ctccgtgatc gtaacgcccg tctcctcttc gacgatcctc atcgaccttc
     4681 tatcggtcgg gtcgctgtag gttcccgcga cccattcatc accctcgccc ggcctggctg
     4741 tgaatcggca ggcgccgagg gccaggcgca ggttcgggtt gtctacggaa gggtaggcga
     4801 cctgtatcgg cgtctccctc atacgaatcc tccttcgcta tggtttcgag tacgtgcctg
     4861 gaacagaggt ggactcagac tacctcgagg ttgatctgcc cgatgccgcc ctggacgtcg
     4921 atttcgagcg tgacatcaga gctgccgtaa gcttcgttca cgtacgagtc gccctccttc
     4981 gttagcccct tggcgttgat gttgccgagg ccgccttcgg ccctggccct cacaccgact
     5041 tcgctgggaa gcataaccgt tgcctccccg accccgccct gtattctggc gtcgaagtca
     5101 ccggtgtaat cacccgtgag gtccaccgtg gtcttgcctg ctcccatctg aagatcgagt
     5161 ccctttagag tgaggctgtc cagatccagc gaactttccc ctgcgcccat ctgcacccgg
     5221 aggtcggtag gtatctcgtc gctgaagagc acatcccact cgttgcgggc gtcgccgccg
     5281 gggcgaacgt cctggccgct accttgctcg acgctcagtt ccccagtgtc tccgctcacg
     5341 tcgtagctta cgtctggctt ccagtccgca acgttgtaag cgaactccgc ctccatcagc
     5401 gcgtcggccc caccggatac gttgagctcg ccagcaccaa tcttgaggtg agcgcgcacg
     5461 ctctgggcgt tctccggcgg gaccttctgc gagtcgcgct gcatctctcc gacggcttgc
     5521 gattggcaag cccctccgag catcgccacg gccgcagctg cgaccaacat gggtaaggaa
     5581 cggttcatcc tat
//
LOCUS       DATMVF010000087        12266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_473694, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000087 DATMVF010000000
VERSION     DATMVF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12266
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473694"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VN178_03835"
     CDS             complement(<1..710)
                     /locus_tag="VN178_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80131.1"
                     /translation="MSEIPNKGVDRRAMAGLSAGHLFTDLNQGAIAAMLPFLIADRGI
                     SLAAAGALLFAATVSSSIVQPLFGIFSDRRPVPVLMPLGVLLAGCGMALVGLAPSYPL
                     ILLCVVLSGIGVAAFHPEAARFANYVSGLGRARGMSFFSVGGNAGFALGPALTTPLIL
                     LFGLPGALFLAVPALIMAAVLFHELPRMLGFKPEAVESEGETVADAPEHWGPFVRMIG
                     VVTVRSFVYFGLVAFVAS"
     gene            complement(753..2447)
                     /gene="sppA"
                     /locus_tag="VN178_03840"
     CDS             complement(753..2447)
                     /gene="sppA"
                     /locus_tag="VN178_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWS80132.1"
                     /translation="MSLIIDALVNLWRSIRNARARLLGRPPEYVWIEVSGPLAEFETP
                     VGFLRRRLSQGPSPPTLERLRLWLDRVSADGRPRGVVLRIRDLDAGWAAIEELRREIL
                     AFRSRGGRVVAYFADPADIRSYYLACAADEVLATPLAELNVVGIRARVDFLKDALDNL
                     GLEAKVVAVSPYKSAGERFVSNDFSRESREQAGRLLDSRFEEVVEAISDGRDLSPEQV
                     RTKIDLAPFGASEALSEGLLDGVLYEDELPARLGSEERPAGLVEWGRARGSLRVPYRR
                     RLRKKVALVTLSGSIVRGRSRRLPIPLPFVGGEQAGSESVVGALRVAEKNRRVGAVLF
                     YVDSPGGDALASDLIWREIERVGAKKPVVVLMGNVAASGGYYVSAAASHVVARRNTLT
                     GSIGVLAIRPVALGLYEKLGINPVVLERGAHAGLLDPSRRPGPDELRVIEAQIGRIYT
                     QFKVRVARGRKMQLSDLEGIAGGRVWTGGEALELGLVDETGGFREALRRARELGGIER
                     DVPGVLTKINPPRGGRPLPGEPARATLDAIGEAWLALSGLRAGAFLAVAPYDISDG"
     gene            2549..2968
                     /locus_tag="VN178_03845"
     CDS             2549..2968
                     /locus_tag="VN178_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HWS80133.1"
                     /translation="MLVPDVREKLRRSGYTLTSQRRAVLEALSRAKGHPSAEDVYLIV
                     KRENPRVALGTVYQALSVLEEVGIIGSKHWAESPTRYDLNVEPHLDIRCISCGEVSEV
                     PGVALDGVEERIRNNTPYRVTRATMVVEGVCPSCTDS"
     gene            3206..5029
                     /locus_tag="VN178_03850"
     CDS             3206..5029
                     /locus_tag="VN178_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80134.1"
                     /translation="MSEDPRDEAAALAEGMEAAGALIENLEQEVADLRSDLDRAAVAL
                     KAAQEEISSRAGALEEKERARVEAESKVEDLRAEIFALEQRHSDEQLRLSNEHINEIA
                     EVRSRLEEQRRTEVDAASSEARLDTIKEEFRREREAMEARYREEIEALKSASEHWEEQ
                     LRTSYQEQEARHAAELEAARTAAADRESVLERSLNEDFERRLADERATADDRQTAAVQ
                     ALRSAAAERELELQNVVETQQGEIESLRAELEAARRSSEERRKQDLRQIKALAEGRES
                     DLRKTHATRLAEAKESADKRVATIQAQREADNRALRARHEEEKMLLERGYEERLAAEV
                     ERRKSETWALDERLREAALQRETEMRTYTARLAELEASRLSQRSSSQADLEQVAERFG
                     AEISDFENRITELEGALEVSETRRNELEGVLDEIRAGHEEPSGIPARPDDAADEEPEG
                     QLEDAQAQTILAENKIEDLEAKLSEAREESRRNAAELQKALESLDRLSDPARRLREGI
                     ALFNESEHARTVASISKAFGLPRVHAALDDATPGRPTLTFLWGDMAWRRYISDPTDGV
                     EEPRVYLTGTGEDPSEVEVPSRQPNARMDSRGRLMIGIQAR"
     gene            complement(5262..5696)
                     /locus_tag="VN178_03855"
     CDS             complement(5262..5696)
                     /locus_tag="VN178_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80135.1"
                     /translation="MRSIMAVGIGLLLALVLGLLVVQGIFAPVLTRFFGLERAGPAAL
                     PLVLLVFAAAFSFYFGGMAASYKAPSRHRLHGVLVVPVAFVLSLVLNLVLGKGFLPGV
                     DGAWALGQVVIFLAVSCAASYVGALRGEALYAHNQKVARRRR"
     gene            5800..7305
                     /locus_tag="VN178_03860"
     CDS             5800..7305
                     /locus_tag="VN178_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWS80136.1"
                     /translation="MRNRTLTRRAFLKMAGAGMASAMLLGATGCGITERIRNITIDPP
                     GIKEGTNVVLIIIDSLRKDHVGAYGNEEIRTPNLDALAKDSLLFSRAYPESIPTICAR
                     RAIHTGLRTWPFRDWVPPKGEDIILQGWQPIPNHQITLAETMQAAGYGTYLVTDNMHQ
                     FKPSYNIHRGFDAFDFFRGQTTDNYRPAWTYPKDKVDQALVKGNIPAMTGQMRQYFAN
                     VKERKTEADWFSPMVFSQAAEYLDVLSKGGPFFLTVDSYDPHEPWDPPEKYVEMYDDE
                     PYNLKEPFSVIYGPSSYLLPRELERMKARYAAEVTMMDRWLGRFLDKMEELDLFDNTL
                     LILLSDHGVAHGEHGYTGKPDNVLWPEVTDIPFYIRHPEGKGAGKTSEYYASTHDVAP
                     TILGSLGIEPQQELEGQDLTVLLGGGEPEARPHFSIGYNDHVWARDDRYVMFSTNQGA
                     DAKLYDVEQDPGMQNDIAGAEPNVVKRMFEGYILRDAGGPLPTYDYGTDAR"
     gene            complement(7331..8113)
                     /locus_tag="VN178_03865"
     CDS             complement(7331..8113)
                     /locus_tag="VN178_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWS80137.1"
                     /translation="MTGERRLQRHVGKIARWIAGSEHMVAFTGAGISTDSGIPDFRGP
                     EGVWTRRDAGLPAPKWRVPPDEVGPNATHLALFELQRLGKLRFLVTQNTDNLHRRSGI
                     SPDSLAELHGNGQLMRCLGCDRLYTRQEVGWDRGRWGRGYRTQKPVPGQPECANCGGR
                     LVSSVVNFGDSLPQKDLMHADHHARRCDLMLVLGSSLMVQPAASLVGLALRSGARVVL
                     VNLGKTPYDRAATLRVWAGIGEVIPPAVEKVKWALGERSDIP"
     gene            complement(8221..8637)
                     /locus_tag="VN178_03870"
     CDS             complement(8221..8637)
                     /locus_tag="VN178_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HWS80138.1"
                     /translation="MSDKSDGHEVHPIVLELARGQNFAALTTLLPGGHPQTQVMWVDA
                     NERHLLINTELHRQKFRNVERDPRVTVTIWDVEDPYRFVEVRGEVVEKIKGQEAREHI
                     DELSHKYRGKPYQTQIQSERVVLRIVPLRQVVRTPT"
     gene            complement(8724..9275)
                     /locus_tag="VN178_03875"
     CDS             complement(8724..9275)
                     /locus_tag="VN178_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWS80139.1"
                     /translation="MNASKLNEAAPMIVRVVQGSLMAGHGAQKLFGSFGGPGLEGTSG
                     FMEMLGMRPGRPWAYLAGLSEFGGGLLTALGFLKPFGPLGVIGSMAMATRKAHWDKPI
                     WVTEGGAELPVLNIAISTALMIRESDGYSLDRLLGIRLPAWVGLVGLVGIILTVLYAE
                     RESDEEVAQQGDQDAAREDLAGP"
     gene            complement(9522..10778)
                     /locus_tag="VN178_03880"
     CDS             complement(9522..10778)
                     /locus_tag="VN178_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80140.1"
                     /translation="MSRRGAAWLAWSVWAFSLTLTALSLVLLALNRSHPEIPIYSFWA
                     ENILFSVGFSTVGAVIVPRMPAENPVGWLFCGIGFLWAIVHFAGEYAIYTLLAAPGSL
                     PAGEVASWLYSWPWVIALGLLVFLALLFPDGRLLSARWRWFARLSALATLVCAVVAAF
                     SPGLPVGLTAIRNPLGIEGLPSLYKAVQVIMLVLTIGGVVSLLVRRLYARGVERQQTR
                     WYTYATAVAVSGAVLEYIISEPLELVWLGWVGHALVLIGILGIPISMGIAVTRYRLYE
                     IDLLVNRTLVYGLLTTTLALVYFASVTATQEILRALTGQEELPQLAVVASTLVIAALF
                     TPLRVRIQTFIDRRFYRSKYDARKTLEAFSAKLKDETDLDRLGEDLVSVVGETMRPAH
                     VGLWLRPDTGWKDDKVERLRVDKKER"
     gene            complement(10881..11810)
                     /locus_tag="VN178_03885"
     CDS             complement(10881..11810)
                     /locus_tag="VN178_03885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80141.1"
                     /translation="MRDEDFDRRVGDVRRFNRFWTRRIGVLRDGYLESPFSLTEVRVL
                     YELAHCDETTAGELGEELGLDAGYLSRILSGFEKHGLIRKRRSQADGRRRLLRLTERG
                     REAFAPLDARSRMEIGAMLGGVSVAGQERLVGAMRTIEGLLGARPEPAVPYLLRPHRP
                     GDMGWVVHRHGVLYASEYGWDERFEALVAEIVAKFIQRYDPRLERCWIAEREGEIVGC
                     VFLVRESEETAKLRLLLVEPEARGLGIGSRLVEECIRFARQAGYRKITLWTNDVLNSA
                     RRIYEGMGFLLVHEEPHHSFGHDLVGQTWELML"
     gene            12078..>12266
                     /locus_tag="VN178_03890"
     CDS             12078..>12266
                     /locus_tag="VN178_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80142.1"
                     /translation="MIRISVQVSSGTARFRVAIQAESIERALENVASQHPGKVCEVMF
                     PIDSETFFVEDPDAPVGQL"
BASE COUNT         2551 a         3950 c         3801 g         1964 t
ORIGIN      
        1 taggaggcga cgaaggcaac gagcccgaag tacacgaaag accgtactgt gaccaccccg
       61 atcatacgca cgaatggccc ccagtgttcg ggcgcgtctg ccacggtctc cccttcgctc
      121 tcgaccgcct cgggtttgaa cccgagcatc cgcggcagct cgtggaacag cacggcggcc
      181 ataatgaggg ccggcaccgc gaggaacaaa gcccctggca gcccgaagag caggatgagc
      241 ggcgtggtca gggcgggtcc gagggcgaag ccggcgttac cgcccacgga gaagaagctc
      301 atcccgcgcg cccggcccaa cccggagacg tagttggcga accgcgccgc ctcggggtgg
      361 aacgcggcca ccccgatacc gctcagaacg acgcagagca ggatcagggg atagctcggc
      421 gccaggccga cgagcgccat tccacacccg gcgagcaaga caccgagcgg cataaggacg
      481 ggtaccggcc ttctgtccga aaaaataccg aacaacggct gtacgatcga cgagctcacc
      541 gtcgcggcga acaagagggc gccggccgcc gccagcgaga taccccggtc cgcgatcagg
      601 aaaggcagca tagcagcaat ggctccctga ttgaggtccg tgaacagatg gccggccgac
      661 agccccgcca tcgcccgccg gtcgacgcct ttgtttggga tctcgctcaa tacaggttcc
      721 ttccgaagtc gcggcagtcg acgaaaatcc tatcagccat ctgagatgtc gtagggcgcg
      781 accgccaaaa aagcgccggc ccgaagccct gaaagggcca gccacgcctc ccctatggcg
      841 tccagtgtgg cccgcgcagg ctctccgggg agaggccgtc cgcccctggg cgggttgatc
      901 tttgtgagca ctcccggtac atcccgctcg atgccgccaa gctccctggc cctacgcaga
      961 gcttctctga aacccccggt ctcgtcgacc agcccgagct ccaacgcctc gccacccgtc
     1021 catacccttc cgccggcgat gccttcgagg tccgagagct gcatcttgcg tcccctggcg
     1081 acacgcacct tgaactgtgt gtagatgcgc ccgatctggg cttcgatcac ccgcagttcg
     1141 tcggggccgg gccggcgact ggggtctaga agaccggcat gcgcccccct ctctagtacg
     1201 acggggttga taccaagctt ctcgtagagt ccgagggcga cgggacggat ggccagtacg
     1261 cctatggaac ccgtgagcgt gttgcggcgt gcgacaacgt ggctcgccgc cgccgagacg
     1321 tagtagccgc ccgaggcggc cacattcccc ataaggacga cgaccggctt tttcgcgccg
     1381 acgcgttcga tctcgcgcca gatcaggtca gaagccagcg cgtccccgcc gggcgagtcg
     1441 acgtagaaca gaacggctcc gaccctccgg ttcttctccg ccacccgcag cgcgccgacg
     1501 acggattcgc tcccggcctg ctcccctccg acgaagggca gcggaatagg cagtctcctg
     1561 ctccggcccc gaactataga tcccgaaagg gtaacgagcg cgaccttctt tcgcaaccgc
     1621 ctcctgtaag gcacacgcaa tgagcctcgt gcccggcccc actccaccaa cccggcgggt
     1681 cgttcctcgg acccgagcct cgccggcaac tcgtcctcgt agaggacgcc gtcgagcagt
     1741 ccttcggaga gggcttccga tgcgccgaaa ggggcgaggt ctatcttggt gcggacctgt
     1801 tcgggggaga gatctcggcc atcggagatc gcctcgacca cctcctcgaa gcggctatcg
     1861 aggaggcgtc cggcctgctc gcgggactcg cgggagaagt cgttgctgac gaatcgctca
     1921 cccgctgact tgtagggcga gacggcgacg actttggcct cgaggccgag gttgtcgagg
     1981 gcgtctttga ggaaatccac cctggcgcgg atgccgacca cgttcaactc ggcgagcggg
     2041 gtggccagca cctcgtccgc cgcgcacgcc agatagtagg atcgaatgtc cgcgggatcg
     2101 gcgaagtagg ccacgacgcg gccgccgcgt gagcggaagg cgaggatctc acgtcgcaac
     2161 tcctcgatgg cggcccatcc ggcatcgagg tctctgatgc ggagcacgac cccgcgcggt
     2221 cgcccgtcgg cggacacgcg gtcgagccac aggcgcagcc tctccagcgt cggcgggctc
     2281 ggaccctggg acaggcgacg ccgcaggaag ccgacgggcg tctcgaactc ggcaagcggg
     2341 ccactgacct cgatccacac gtactccggc ggcctgccga gtagacgcgc gcgggcgttt
     2401 cggatcgagc gccagaggtt gaccagggca tcgatgatca gcgacatctg gcagtatggt
     2461 acaccgcagc acgaaaaacg ccgtatgata aactctaaaa tcaggaatgg ttactgatca
     2521 caagccaacg caggaagata cgtcaggcgt gctcgtaccc gacgtcaggg agaagctacg
     2581 caggtctggt tataccctca cctcccagcg cagggccgta ctcgaggccc tctccagggc
     2641 gaagggacac ccttcggccg aggacgtcta ccttatcgtc aagcgcgaga acccgcgggt
     2701 ggcgctgggc acggtctacc aggcgctctc ggtactggaa gaggtcggca taataggctc
     2761 gaaacactgg gccgaatccc cgacgcgcta cgacctcaac gtagagccgc acctggacat
     2821 caggtgcatc agttgcggcg aggtctccga ggtccccggg gtcgcactcg acggcgtgga
     2881 agagcgcatc cgcaacaata ccccttacag ggtcacccgc gcgacaatgg tcgtcgaggg
     2941 cgtgtgccct tcctgcacag acagctagtc agcatgtcaa ctactcctcc ctagcctccc
     3001 caccagcgta aaaccaaccc cgccccgagc agcggttctc tcatgctgac gagcgaggcc
     3061 cgaagggctg agcgtgctga aaagctgaag tgctcgcaaa gaggtccttg atctctttgc
     3121 gaccgtgcag ggtctttacc ccaagcctgc caggagttac cctttcgacc aacgtgaagt
     3181 tgcgggtaac acggaagagg cgatcatgag cgaggatccc agggatgaag cggccgcgct
     3241 cgctgagggc atggaggcgg ctggcgccct gatcgagaac ctggagcagg aggtggccga
     3301 ccttaggagc gacctggacc gggcggccgt ggcgctgaag gctgcgcagg aggagatctc
     3361 ttcccgggcc ggggcgctgg aggagaaaga gcgggcccgc gtcgaggccg aaagtaaggt
     3421 cgaagacctc agggcggaga tcttcgcact cgagcagcgg cactcggacg agcagctccg
     3481 cctcagcaac gagcacataa acgagatcgc agaggtgcgt agcaggctcg aagagcaacg
     3541 acgcacagaa gtggacgcgg cctcctcgga ggctcgtctc gacactatca aggaagagtt
     3601 caggcgtgaa cgcgaagcca tggaggcgcg ctacagggag gagatcgaag ccctgaaaag
     3661 cgcctccgag cattgggagg agcagctaag gacgtcttac caggagcagg aggcgcgcca
     3721 cgcggccgag ctcgaggcgg cccgcaccgc ggccgccgac agggagagtg tcttggagcg
     3781 ctccctcaat gaagacttcg aaaggaggct ggccgatgag cgggccaccg ccgacgatcg
     3841 tcagacggcg gccgtgcagg ccctgagaag cgcggcggca gagagggagt tggaactcca
     3901 gaacgtcgta gagacgcagc aaggggagat agaatcgctg cgcgccgagc tcgaggcggc
     3961 ccgccgcagc tcggaggaac ggcgcaaaca ggatctccgg caaataaagg cgctcgccga
     4021 ggggcgcgag tccgacctga ggaagaccca cgcgaccagg ctggccgagg cgaaagagtc
     4081 cgcagataag agggtcgcga ccatccaggc ccaaagagag gccgacaaca gggcgctcag
     4141 ggcgcgccac gaggaggaga agatgctcct cgagcgcggg tacgaggagc gactggcggc
     4201 cgaagtcgag cgcaggaagt cggagacttg ggcgctcgac gagcgattga gggaagccgc
     4261 ccttcagcga gagaccgaga tgcgcaccta caccgcacgc ctggcggagc tcgaggcgtc
     4321 caggctgtcc caaagatcct cctcgcaggc ggatctggag caggtggcgg agcgtttcgg
     4381 ggcggagatc tccgatttcg agaaccgcat caccgagctg gaaggggccc tcgaagtgtc
     4441 cgagacccgc aggaacgaac tggagggagt actcgacgag atccgtgccg gccatgagga
     4501 gccgtccggg atcccagccc ggcccgacga tgccgcagac gaagaaccgg aagggcagct
     4561 ggaggatgcc caggcgcaga ccatactcgc cgagaacaag atcgaagacc tagaagcgaa
     4621 actctctgag gccagggaag agagccggcg caacgctgca gagctacaga aagcactgga
     4681 gagcctcgac cggctctccg acccggcacg ccggctcagg gaaggcatcg cgctcttcaa
     4741 cgagagcgag cacgccagga cggtagcttc catctcgaag gccttcggcc tacccagggt
     4801 tcacgcggcc ctcgacgatg cgaccccagg aagaccaacg ctcacgttcc tctggggcga
     4861 catggcctgg cgacggtaca tctcagaccc gacagatggg gtcgaggaac ccagggtcta
     4921 cctcacgggt acgggcgaag acccgtccga agtcgaggtg ccgagccggc agcccaacgc
     4981 caggatggac tcgagggggc gcctaatgat cggtatccag gcccgctgaa gcggtatggg
     5041 gaaggataaa ccttccccat accgcgctgg tcagcacggc ggctttgccg ccttatcagc
     5101 atgtcagcca gctcgcggat ggcggaggga aggttctatg gagaggcttt gtatcgtggc
     5161 gaacgattcg cgatcgagca caggcagcga gccgacacca ggaaagccga aatgctgaag
     5221 tgctgacaag agaaggctaa aagcctgagc gtgctgaaat gctaccgacg gcgccgcgct
     5281 accttctggt tatgtgcata gagcgcctcc ccgcggaggg cgccgacgta ggaggccgcg
     5341 caggagacag cgaggaagat cactacctgt cccaaggccc aggcaccatc tacgccgggc
     5401 aggaatcctt tacccaatac caggttgagc accagggaaa gtacgaaggc cacggggacg
     5461 accaggaccc cgtgcagacg atgtctggaa ggcgccttgt agctggcggc catccccccg
     5521 aagtagaacg agaaggcggc ggcgaagacg agcaagacga gcgggagggc ggcggggccc
     5581 gcgcgctcga ggccaaagaa acgtgtgagg accggcgcga atatcccctg caccacgagc
     5641 aaaccgagca cgagcgccag tagcaacccg atcccgacgg ccatgatgct tctcatacga
     5701 atcattctag cagtccgttt ggcgggccag ggtgggcggg ttcacggtgg cggccgcccg
     5761 gcttgtaggt cgcattgttg cgtagtacgc tcttgcttcg tgagaaaccg cactctgacc
     5821 cgcagagcct tcctcaagat ggccggtgcc gggatggcct cggcgatgct gctcggtgcc
     5881 acaggctgcg gcattacaga gcgcatccgc aacatcacca tagatccccc cggtatcaaa
     5941 gagggtacca acgtggtgct cattatcatc gacagcctga ggaaggatca cgtcggcgcc
     6001 tacggcaacg aggagatccg gacccccaac ctcgacgccc ttgccaagga cagcctgctc
     6061 tttagcaggg cttaccccga gtccataccg acgatctgcg cgaggagggc catacacacc
     6121 ggcctcagaa cctggccctt cagagattgg gtgccgccca agggtgagga cataatcctg
     6181 cagggctggc agccgatacc caaccaccag atcacgctcg ccgagacaat gcaggcagcg
     6241 gggtacggga cctacctcgt gacagacaac atgcaccagt tcaagccctc ctacaatatt
     6301 catcgtggct tcgacgcctt cgacttcttc cgcggccaga ccacagacaa ttacaggccg
     6361 gcgtggacct acccgaaaga caaggtcgac caggccctcg tcaagggcaa catccccgcc
     6421 atgacgggcc agatgcgcca gtacttcgcc aacgtgaagg agcgtaagac ggaggccgac
     6481 tggttctccc ccatggtctt ctcccaggcc gcagagtacc tcgatgtcct gagcaagggc
     6541 gggcctttct tcctcaccgt ggacagctac gatccgcacg agccctggga cccacccgag
     6601 aagtacgtcg agatgtacga cgacgagccc tacaacctca aggaaccgtt ctctgtgatc
     6661 tacggcccga gcagctacct gctgccgagg gagctggaga ggatgaaggc gcgctacgcg
     6721 gcggaggtca ccatgatgga ccgctggctg ggccgtttcc tcgacaagat ggaagagctt
     6781 gacctcttcg ataacaccct gctcatactg ctctccgacc acggtgtcgc ccacggcgag
     6841 cacggttata cgggcaagcc ggacaacgtg ctctggcccg aggttacaga catcccgttc
     6901 tacataaggc accccgaagg caagggggcc ggaaagacca gcgaatacta cgcctccacc
     6961 cacgacgtgg caccgacgat cctgggctca ctcggcatcg agccgcagca ggagctggaa
     7021 ggccaggacc tcaccgtcct gctcggtgga ggggaacccg aggcgagacc ccacttctcg
     7081 atcggctaca acgaccatgt ctgggcccgc gacgaccgct acgtcatgtt cagcaccaac
     7141 cagggcgccg acgccaaact ctacgacgta gagcaggacc ctggtatgca aaacgacatc
     7201 gcaggggccg agcccaacgt agtaaagcgg atgttcgaag gctacatact aagagacgcc
     7261 ggcggccctt tgcccaccta cgactacggc accgacgcca gatagagcgc tccagcgccg
     7321 taccccgtgc tcaaggaata tccgagcgct ccccgagcgc ccacttcacc ttctccaccg
     7381 caggcgggat cacctcgcct atgcccgccc aaacccgtag cgtcgccgcc ctgtcgtagg
     7441 gcgtctttcc caggttgacc aggaccaccc gagcacccga tctgagcgcc aggccaacga
     7501 gcgaggctgc aggctgcacc atgagcgagg agccgaggac gagcatcagg tcgcagcggc
     7561 gggcgtggtg gtcggcgtgc atcaagtctt tctgcggcaa cgagtccccg aagttcacca
     7621 cagaggagac gagacgtccg ccgcaattgg cgcactcagg ctggcccggc accggcttct
     7681 gggtgcggta gccgcggccc caccggcccc tgtcccaccc tacctcctgc ctggtgtaga
     7741 gccggtcgca acccagacag cgcatgagct gcccgttgcc gtgtaactcg gctagagaat
     7801 cggggctgat gccggagcgg cggtggaggt tgtcggtgtt ctgcgtgaca aggaaccgga
     7861 gtttgccgag ccgctggagt tcgaagagtg ccagatgggt ggcgttcggc cctacctcat
     7921 ccggtggtac acgccacttt ggtgccggta gaccggcgtc gcgccgcgtc cacaccccct
     7981 cgggcccccg aaagtcgggg ataccagagt cggtgctgat accggcgcca gtgaaggcga
     8041 ccatgtgctc cgacccggcg atccaccggg cgatcttccc cacgtgccgc tggaggcgtc
     8101 gttcgccggt catggtctcc gccacatggc cgctagacta tcattggagt gtaccggcct
     8161 ctacccttgc cggtcggccg gtgcggcgtc tggctcatgg cggaagtcgg ggatggtaac
     8221 tcaggtgggt gtgcgtacta cctgtcgtaa ggggacgatc ctgagaacca cccgttcgct
     8281 ttggatctgt gtctggtaag gcttgccacg gtatttgtgg gacagttcgt cgatgtgctc
     8341 tctcgcctcc tgacccttta tcttctctac gacctcccca cgcacttcga cgaagcggta
     8401 gggatcttcc acgtcccaga tagtgacggt caccctcggg tctcgttcga cgttgcgaaa
     8461 cttctggcgg tgcaattcgg tgttgatcag gaggtgccgc tcattagcat cgacccacat
     8521 cacctgcgtc tgcgggtggc caccgggtaa cagcgtagtg agtgccgcaa agttctgtcc
     8581 gcgggccagt tcgagaacga tcggatgaac ctcgtggcca tctgatttgt ccgacatgcg
     8641 ctcctctccc cgctcgggtg tcgattacgc ttgtagtacg ttagtcctgc acagccaaat
     8701 tggcgcgagg cacccagccg cgattatggt ccggccagat cctcgcgggc cgcgtcctgg
     8761 tccccctgct gcgccacttc ttcgtcggat tcccgctcgg catagagcac ggtgagtatg
     8821 atcccgacga ggccaacgag cccgacccag gccggcaggc ggatacccaa gagtcgatcc
     8881 agggagtacc cgtcggactc gcggatcatg agcgccgtcg agatggcgat gttgaggacg
     8941 ggcagttcgg cgcctccctc ggtcacccag atgggcttgt cccagtgggc cttcctggtg
     9001 gccatagcca tggaacctat gacaccgagg gggccgaaag gcttgaggaa ccccagggct
     9061 gtgagcaagc cgccaccgaa ctccgagagt cccgccagat aggcccaggg gcgacccggg
     9121 cgcataccga gcatctccat gaagccgctg gtaccctcta accctggccc cccaaaagac
     9181 ccgaatagct tctgggcccc gtggcccgcc ataagcgagc cttgcaccac acgcacgatc
     9241 atcggcgccg cttcattgag tttggaggcg ttcatcctca ctccttctgt atcgtgtctt
     9301 accaccctcg tccgccagag acggggcgct tgcctacgct ctataccctg gaccaccggt
     9361 ttacactcct gcatttgtgc caaaggggtc tggccggtga ccaactcatg gtctatcttg
     9421 cttaggtatg gggttaggag gtttcgcgct caccgaggag ctcctcaggg agatacccaa
     9481 ggcggagctg cacattcaca tcgaaagttc atgcaacaaa actagcgctc cttcttatcc
     9541 actcgtaacc tctccacctt atcgtctttc caccccgtat ccgggcgaag ccacaggcct
     9601 acatgagcgg gccgcatagt ctcgccgacc acgctcacca gatcctctcc gagcctatcg
     9661 aggtctgtct cgtccttgag cttggcagag aaggcttcca gggtcttcct cgcatcatac
     9721 ttgctgcggt agaagcgacg atctatgaac gtctggatac gtaccctcaa gggggtgaat
     9781 agcgcggcta tgaccagcgt ggaagcaacg acggcgagct ggggcaactc ctcctggccg
     9841 gttagcgccc tgaggatctc ctgggtcgcc gttacgcttg cgaagtacac tagtgccagg
     9901 gttgtcgtca gtaaaccgta gaccagggtg cggtttacga ggaggtctat ctcgtagagc
     9961 ctgtagcgcg ttacggcgat ccccatggag atcggtatgc ctaggatgcc gatcaggaca
    10021 agcgcgtgtc caacccatcc gagccacacc aattccagcg gctcggagat gatgtactcc
    10081 aggacggcac cgctgaccgc cacagcggta gcataggtgt accatctggt ctgctggcgc
    10141 tctacccctc tcgcataaag ccggcgcacc aacaacgaga cgaccccgcc gattgtcagg
    10201 acgagcatga tcacttgcac agccttgtaa aggctcggca ggccctctat accgagcggg
    10261 ttgcgaatgg cagtgaggcc gacaggcaga ccaggagaga aggccgccac aaccgcacac
    10321 accaacgtcg cgagtgcgct gagacgcgcg aaccacctcc agcgggcgct tagcaaccgg
    10381 ccgtcgggga acagaagggc caaaaacacc aggaggccga gcgcgatgac ccagggccag
    10441 gagtagagcc agctcgcgac ttcaccggcc gggagcgatc ctggcgccgc cagtagcgtg
    10501 tagatggcat actcgccagc gaaatggact atcgcccata agaagcctat cccacagaaa
    10561 agccacccta cagggttctc ggcgggcata cggggaacta tcactgcgcc cacggtcgag
    10621 aagcctaccg agaacaagat attctctgcc cagaaggagt agatggggat ctcaggatga
    10681 gacctattca gggccagaag aacaaggctc agggcggtca aagtcaggga gaaagcccag
    10741 accgaccagg cgagccaggc ggcaccgcga cggctcatcc caccggtact gcgaccccgt
    10801 tgccttgtcc cttctgtcac aacagcactc tacaccacga ccgcctccaa agcgttacgg
    10861 aaggagtctt ctcgagagaa tcacagcatc agctcccagg tctggcctac cagatcatgc
    10921 ccaaagctgt ggtgtggttc ctcgtggacg agcaggaagc ccatcccttc gtagatgcgt
    10981 cgggcggagt tcagcacgtc gttcgtccac aaagtgatct tccggtagcc ggcctgccgg
    11041 gcgaagcgga tgcactcttc caccaggcgg ctgccgattc caagcccgcg tgcttccggc
    11101 tcgacgagga gaaggcgcag cttggcagtc tcctccgact ccctgacgag gaacacacaa
    11161 cctacgatct cgccttccct ctcagcgatc caacaacgct ccagtctcgg gtcataccgc
    11221 tggatgaact tcgccacgat ctccgcaaca agcgcctcga atcgctcgtc ccagccgtac
    11281 tcactggcat agaggacgcc gtggcggtgg acgacccagc ccatgtcgcc cggccggtgg
    11341 ggcctcagaa ggtaagggac cgccggctcg ggccgagctc cgagtagccc ctcgatggtt
    11401 cgcatcgctc ctaccagacg ttcctgcccc gctacagaca cgcccccaag catcgcgccg
    11461 atctccatgc gcgaccgtgc gtcgagcggg gcgaaggcct cccgacctcg ctccgttagc
    11521 ctcaggagcc ggcgcctgcc gtcggcttgg gagcgtctct tgcggatcag gccgtgcttc
    11581 tcgaaaccgc tcagaatgcg gctcaggtag ccagcgtcca atcccaactc ctcgccgagt
    11641 tcgcccgccg tggtctcatc gcagtgagca agctcgtaga ggacgcgtac ctcggtcagg
    11701 gagaaaggac tctccagata cccgtctcgc aacacgccga tccgccgcgt ccagaaacgg
    11761 ttgaagcgcc ggacatcccc tacccgccgg tcgaaatctt cgtccctcat cggaccacca
    11821 ccagtctcca ccttctcaaa catatgctat tgtcaactaa gtacttgatt ttgtcaagaa
    11881 atttgcagtg tttggcgtgt gatgtgggaa gggcctgcaa gcagaccatc gcgggtagga
    11941 gcccatcctc gacctccggc ctatataccc tccattctcc cgctttcgac aatgtttcac
    12001 tactctcagt gaggatcatg aagtataatc gaggagtaat gatcatggct atctagcgcg
    12061 gaaagagggt gtggggaatg ataagaatct cggtccaggt gagcagtgga acggcgcgtt
    12121 tcagggtggc gatacaggcc gagagcatcg agcgagccct ggagaacgtg gcgagccagc
    12181 acccgggcaa ggtctgcgag gtgatgtttc ctatagactc cgagactttc ttcgtcgagg
    12241 acccggacgc cccggtcggg cagctg
//
LOCUS       DATMVF010000088        16879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_8895, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000088 DATMVF010000000
VERSION     DATMVF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16879
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8895"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VN178_03895"
     CDS             <1..342
                     /locus_tag="VN178_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HWS80143.1"
                     /translation="FLEMRNLVEDVYTSEAVLRYVVSVTSATRGRDDVYLGASPRASR
                     MLLAASQARAAANGRSYCAPDDVKAMASSVLSHRIIPSATSSEDGIGPDNATREIVQD
                     ILRSVPVTEAV"
     gene            349..1596
                     /locus_tag="VN178_03900"
     CDS             349..1596
                     /locus_tag="VN178_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWS80144.1"
                     /translation="MPGRGASRLSIRPTARGWQALVAGVVTLVVARLIGTTQFHQLAY
                     ALLALPLASLVLGVVGTRGLLYSRVLPPGIRITAGSPARLRLLLERSRFGAAKASVVD
                     RLPGPRSFDFSATRERSRIEVPLTFARRGVYTLGPAEVRVADPFGLLRFSRRDRETTE
                     VVVYPQVYELRGFPLRGGNVEAGTTGSRGRRGEEFANLREYRRGDDRRHIHWKSLART
                     GELFVKEFSLQAPRRHTVVLDLRREGLRTQENEIEDAVSVAASVLTHLAEEGLPFRLL
                     DTGSSGTDTGFGSDEDSYWEAMRHLATARADGSRHLAETVLGERGGLGEGVVLVSRTR
                     DGELPHCVRKLREAGLSVIVVALATHTYRTPPVPGSAAQGREAEFLRSVGTMEAAGAT
                     VRIVSHPAGAAGLSGARGVGAVR"
     gene            1593..3845
                     /locus_tag="VN178_03905"
     CDS             1593..3845
                     /locus_tag="VN178_03905"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HWS80145.1"
                     /translation="MNGLATRLWLYGAVLATGSAFSILFTGETYDGADGGFGALSVVT
                     VIAGHKALLPMFFAAVVAALVGSAGRWRFVLLLPAIAAYTVVAVYGTDLFSVSGWQES
                     FRIVGGDVYGAANTMYVQPVPYDLAPGLFVVLIPLVMILVAFATSATLYEESPVISVA
                     VLGLTIGALSTISFEDGAGPFFAVFLVCAVGLFLSTGVAGPDGPGRPAVVAGAIVVAL
                     VLLVPRMPLSDQTVSAGLIDWTRIGTGGTSRLGVQADVGDYLTAGREAELLRIRSSEP
                     LLWRGGTMDYFDGVRWSDTTQPAADDGEEIAPDVETRPVLQRVQVLNAQTDLIFGGYK
                     IVRTSEYRASPNSDGSWSTAEPFEEGDFYEVFSEIPQPTGAQLRGAGSVYPSSVEEKF
                     LQLPEDTPPVVSETAQKIQRRYDPATPYDRARAIERYLIYDGGFTYNLDVSYRRADKA
                     VEEFLGEGKEGFCTQFSTSMALLLRDMNVPSRVVYGSTSGEEVSQGEYLVRGSNMHTW
                     VEAYFPGVGWYPFNPTPGFSMPDAMEANAPRPELPVASGIQAAENSQDRSQLAQNRAQ
                     QRAREQNQNQADQGTTTDRSGQITLPVWPLFVLVPPLIVAVVPASKRALLSRGRPEDL
                     YRDLTGRLRDVLPPNRSIVADSPALTPTERVLLLSGAAGVEEAPMRRFALAYSDHLYS
                     ADGGRDHIVSAYRDAMRAYERLPRWRRALGAVNPASLLARSRKYVTAQKARLGKMLRG
                     RAGRTSRKRR"
     gene            3910..4254
                     /gene="erpA"
                     /locus_tag="VN178_03910"
     CDS             3910..4254
                     /gene="erpA"
                     /locus_tag="VN178_03910"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HWS80146.1"
                     /translation="MEQKEATIINITDNAAEKVKTLMAQEGEEDLALRIGVRPGGCSG
                     FQYSIYFDDEVADDDEVFETKGVRVLVDAMSVPYITGSEFDYVDGLMGAGFAVNNPNV
                     QGGCGCGSSFTC"
     gene            complement(4599..5153)
                     /locus_tag="VN178_03915"
     CDS             complement(4599..5153)
                     /locus_tag="VN178_03915"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWS80147.1"
                     /translation="MAEGGGWKRLRSERLMETPYFSLRSDRLQLPDGGIKDPYFVIER
                     PDAAIIFPLTAGGEVVLVNQYRPPLETMELGLPAGLVEEGERPEEAARRELSEETGYT
                     GGEWEQLGSVASSPSLKDNRAYLFLARGVGESTAPDPDEHELVEVVRVPVEDLLNLVR
                     SGKIVSSSGVAAIMLALERLREDS"
     gene            complement(5146..5538)
                     /locus_tag="VN178_03920"
     CDS             complement(5146..5538)
                     /locus_tag="VN178_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80148.1"
                     /translation="MTRRILAAVEDLMFRSKISETASQLGIEATFPRNPRRLLEAMHE
                     SPPDLLVLDLNSARFEPLTLLEDVRSDEATRDVPTVGFLSHVQKDLAVAAREAGCDRV
                     VARSAFTKDLPQILAGRTPGTIDEAGVG"
     gene            5610..7427
                     /locus_tag="VN178_03925"
     CDS             5610..7427
                     /locus_tag="VN178_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS80149.1"
                     /translation="MNRLKELRAGLRRTAETLSNFTPFLRPRRGGLVLALGVLLLETL
                     TSLAQPWPLALTIDYVLTDNKSLPEAVPAYLEDKAVLLAGIAFLIVLIFTVTRSVASF
                     RRYLLQKLGQETVFDMREALYAKVHALGLDYHGKKRTGDTITRVTSDVKEVRTMLVDS
                     VVEVFSSVMILLGMLVVMLWLDWQLTLLALVTVPFLFLAVKRYRQALVQRMRIVRTRE
                     GAIASVMQEAIIGIKAVKLFAREEDEMGRFRKESRESLKASVDSAVIEAKFSTVLGIV
                     GGLGTALVVYFGARQVLSGALSLGDLTVFVAYLALFLSPLWALSRQVNQIGTSLVSGE
                     RIIELLNAEPTVKDLPGARPAPAFDGRVTFEGVRFAYENEAGEVLRGMDFDVEAGSRV
                     ALVGVSGAGKTTVTSLIARLYDPQAGRVLVDGEDVKEFTLKSLRDSVTFVPQDPLLFR
                     ATVAENIAYGRPGASREEIEAIAELAGADSFIRELPEGYDTVLSERGESLSGGQRQRI
                     SIARAMLRDTPILILDEPQAGLDAEAAAAVEESWRALTEGRTTFVIAHELRLVRSVDS
                     IFVIEDGSVAEAGTHEDLVSSDGLYARLYSLQERDPVQP"
     gene            complement(7780..8814)
                     /locus_tag="VN178_03930"
     CDS             complement(7780..8814)
                     /locus_tag="VN178_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HWS80150.1"
                     /translation="MTTVHITSFFEEEYVRRIREVDERLQVLYREDLVPPPRWPGDHA
                     GPEEWERTPEGDEEFLAMLGEAEVLYDFPRGHVEDLVEVAPRLRWVQGSMAGAGEVAQ
                     KAGLGETDIVVTTAGGVYSGPLAEFVLMAMLQYAKELDHLRRDKTEKIWRQGAVGSLE
                     NKTLCVLGMGSIGRAIATRARPFGMRIIGVKRTVREDDEAWDYADDLYPTAELHAALG
                     EADYVAVTLPGTPETRRLLDAGAIAEIKQGAYFANVGRGSVVDEAALVEALQGGHLSG
                     AALDVFEVEPLPEESPLWELDNVIVSAHTTDVVPALINAAQTDLFCENLRRYLAGEEL
                     LNVLDKRLLY"
     gene            8971..9597
                     /locus_tag="VN178_03935"
     CDS             8971..9597
                     /locus_tag="VN178_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWS80151.1"
                     /translation="MDELLDILDESGLPTGEVVPKGEAHRLGLWHRCFHCWICGSDPE
                     GSYLLLQRRAATKDTWPGYLDVTAAGHLASGEETVDGLREVEEELGLRIEPERLIPLG
                     TRRVEQEIPDGCDRELHEVFLVCDATSPGDLRLQKEEVEAVFRLDLDEVQALYEMGSA
                     PAREYAEGRISATRIHLSEFVPKEEGYLRRVAGAARQLLSDTPPDPIF"
     gene            9751..11475
                     /locus_tag="VN178_03940"
     CDS             9751..11475
                     /locus_tag="VN178_03940"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWS80152.1"
                     /translation="MRVALAQINTTVGDIWGNVEKMADTLERATDSGAELVAYPELTI
                     PGYPPEDLLMRQSFIEENVRALHEFAGRVPENVVAAAGFVDLDADLYNACALLSGGGV
                     LYRYHKRYLPNYSVFDENRYFREGSGAPILGLGEALVGVSVCEDIWYPGGPAREQALG
                     GASVLLNISASPYHRRKGAARERMLSVRASDYGCYVLFCNLTGGQDELVFDGHSVVFD
                     PEGRLVARAGQFREDLLLVDLYPDHSLMQRLHDPRPRKENAEHKPEVVRVPRHETPAV
                     QPVEPRIEPSLPEEGEVLEALVLGLADYFRKNGFSRAVLGLSGGIDSSLAAALAVEAL
                     GPRNITGVLMPSRYTSEGSNTDAAALAKNLGIDTQTIPIGPAFDAYREMMEGAFKGLP
                     EDVTEENLQSRIRGNILMALSNKFGWIVLSTGNKSEMSVGYSTLYGDSAGGFAVIKDV
                     PKNLVYRVARHFNERKNREIIPDSVLTKEPTAELRENQRDTDSLPPYEVLDPILEAYI
                     EDDRGVAEIVASGFDEDDVRRIVQLVDRAEYKRRQAPTGIKVTERAFGRDRRMPITNR
                     YFERNHKE"
     gene            complement(11549..13162)
                     /locus_tag="VN178_03945"
     CDS             complement(11549..13162)
                     /locus_tag="VN178_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS80153.1"
                     /translation="MRDPRVPLIPPTAVLACWALGALVFAFLPQGSEAKSVIATAFSL
                     STAAFAAGILLRAAPRADGHEQAFLRLMGLGMVCRLAGNSLWSASHHLGLGFVTPVAP
                     QDVAYAISYALLVGAMLHLVALTTPRITLVSALDAGAVMLSVGTLVWYFVLGPAATEA
                     GLGSVREAAAALSQPVCDAALLFLGLVVASASIRLRFSTFLNGGFVVLLLADAAYLDL
                     RSVGPYQSGNWPEMLWALGMILLGLTSTTSPEVPADSNVGRIQPWRIALYWLGPLSPP
                     MHFIILLVWGAMNPPLPAYVLAGCAVLLLYLALRIGLVSSVSKHLGQEREESARIKEQ
                     GRLLYELHDTAKQEVHGISLTLQAALDTERRGDREEALEMFGRVLAASREAEFRISRP
                     YDELSALRGESPPGAADFLRQRLRKFEEYFGVKTHDDIQASLEVLNPAETAALIRVFV
                     EAFWNVAKHSGAGNMYLESRRVGPVLIVRVRDDGRGFDRREPPPGLGLEYMRRRAGEV
                     GADLDVISTPGRGTAVQVRFLKRRYTADS"
     gene            13316..13477
                     /locus_tag="VN178_03950"
     CDS             13316..13477
                     /locus_tag="VN178_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80154.1"
                     /translation="MEYARLGNTGLMVSELCLRCMTFGQEADEKTSKEIVATDFGLRA
                     VMGYLPERS"
     gene            complement(13479..13715)
                     /locus_tag="VN178_03955"
     CDS             complement(13479..13715)
                     /locus_tag="VN178_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80155.1"
                     /translation="MAQCEVCGNEYDKAFQVSVADGDSHTFDSFECAIHALAPVCEYC
                     NVKVIGHGTEEGGSFYCCAHCARMAGAEGVSDRA"
     gene            13975..14433
                     /locus_tag="VN178_03960"
     CDS             13975..14433
                     /locus_tag="VN178_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80156.1"
                     /translation="MKRIITLSTIAALMLGVLAAGVVYANNEDAGAKKCSVATLHGTY
                     LFAFDGVEIKGNDQRPFAIAGYAVFDGNGKEKGVASSNSNGEVTRNERVSATYTVKAD
                     CTGTVTFGDGSQIDMFIAPDGSQFAFVQTKPSKLVGSGFEPRGTAKRVGD"
     gene            complement(14888..15295)
                     /locus_tag="VN178_03965"
     CDS             complement(14888..15295)
                     /locus_tag="VN178_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80157.1"
                     /translation="MDKSSVRNELFAMAKEVGEFEVFPTPSEAEEDPDSAFLISTGIL
                     LGVPKLYIVRVEEFHHSEEEADKETDTILQEQEGHIKRVLSERGIALSEAAWNTVFSY
                     AVWDEHPSRTSGRLSEDEVRSVEEAFDALGLDP"
     gene            complement(15365..15649)
                     /locus_tag="VN178_03970"
     CDS             complement(15365..15649)
                     /locus_tag="VN178_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWS80158.1"
                     /translation="MEGSPHEIDEVLREIREHVLPLLQQQDGFKGFITLEDRQSGKLI
                     GITFWESEQAMQASEEVGVRTRSETAEVTGDAIEDVERYEVGLFEMLGQL"
     gene            15929..16534
                     /locus_tag="VN178_03975"
     CDS             15929..16534
                     /locus_tag="VN178_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80159.1"
                     /translation="MNGRRIMNVEAAMVVRAVGGALLIAIVAAVIVTLVSLAMFLREP
                     IYEPTARVWVERDLRVGPPAPGPDTRLSETTPAMVRLAASRPVANETRQSLGLQASSA
                     ELLDNLEIRRVPDTTFMDLTYRGADRKMAAQTVNTFARVASERLSDAVGKNLSAVVWE
                     EARVPPAVPEPKPLRNGLLTLVGVWALYAGLTLATLVVLRR"
     gene            complement(16663..>16879)
                     /locus_tag="VN178_03980"
     CDS             complement(16663..>16879)
                     /locus_tag="VN178_03980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80160.1"
                     /translation="GRGIGTALMEACLDLADNWLKLTRLDLRVYTDNEPAIALYEKFG
                     FEIEGTHRRFAYRNGEYVDAHIMARLR"
BASE COUNT         3087 a         5349 c         5379 g         3064 t
ORIGIN      
        1 tttttggaga tgcgcaatct cgtcgaggac gtctatacga gtgaggccgt actcaggtac
       61 gtcgtctccg tgacctcggc gacccgggga cgcgatgacg tctatctcgg cgcctcccca
      121 cgggcgagca ggatgttact ggccgcctcg caggcgcggg cggcggcgaa cgggcgttcg
      181 tactgcgcgc ccgacgacgt caaggcgatg gcctccagcg tactctcgca caggatcata
      241 ccctcggcca ccagctccga ggacggcatc gggcccgaca acgccacccg tgagatcgtt
      301 caggacatcc tgcgctccgt ccctgttacc gaggcggtgt aagggcactt gcccgggagg
      361 ggcgcctcgc ggctctccat caggccgacg gcccgcggct ggcaggcgct cgttgccggc
      421 gtcgtcacgt tggtcgtggc gcgtcttatc ggtaccaccc agttccacca gcttgcttac
      481 gcgctccttg ccctgccgct cgcatccctc gtcctcgggg tggttggcac caggggcctt
      541 ctttactcgc gcgtcctgcc tcccggtatc cgtatcacgg ccggcagccc cgcgcggtta
      601 cggctcctac tagagcgctc gcggttcggc gcggcgaagg cgagcgtggt cgaccgtctc
      661 cccggaccgc gcagcttcga tttttcggcg acgagggaga ggagcaggat cgaggtcccc
      721 ctgaccttcg cgcgcagggg cgtctacacc cttggccccg cggaggtcag ggtggcagac
      781 ccattcgggt tgctccggtt ctccaggagg gacagggaga cgaccgaggt ggtcgtctac
      841 ccgcaggtct acgaacttcg gggctttccc ttgcggggcg gaaacgtgga ggccggcacc
      901 acggggtccc gcggccggcg gggcgaggag ttcgcgaacc tgagggagta ccgcaggggc
      961 gacgacaggc gtcacatcca ctggaagagc ctggcacgca cgggggagct tttcgtcaag
     1021 gagttctccc tgcaggcccc gaggcgccac accgtggtcc tggatctgcg cagggagggg
     1081 ctgaggaccc aggagaacga gatcgaggac gccgtctcgg tggcggcctc ggttctcacg
     1141 caccttgccg aggagggtct gcctttcagg ctgctcgata cgggcagtag cggcacagac
     1201 actggtttcg gatccgacga agattcctac tgggaggcga tgaggcacct cgcgacggcg
     1261 cgcgccgacg ggagccgtca cctggcggag accgtgctcg gggagcgggg gggactcggg
     1321 gaaggcgtag tcctcgtctc gcgcacccgc gacggcgagt tgccgcactg cgtccgcaag
     1381 ctcagggagg ccggtctctc tgtgatcgtg gtcgccctcg ccacccacac ctacaggacg
     1441 ccccctgtgc cggggagcgc cgcacagggc cgcgaggcgg agtttctcag gagcgtgggc
     1501 accatggaag cggccggggc cacggtacgc atcgttagcc accctgcggg agcggcggga
     1561 ctctcgggcg ccaggggggt cggggccgtg cgatgaacgg attggcgacg agattgtggc
     1621 tctacggagc ggtgctcgcg acgggcagcg ccttcagcat cctgttcacg ggcgagactt
     1681 acgacggtgc cgatgggggt ttcggggcgc tctccgtggt cacggtcata gccgggcaca
     1741 aggccttgtt gcccatgttc ttcgcggcgg tcgtggcggc gctcgtcggc tcggcgggca
     1801 ggtggcggtt cgtgcttctc ctccccgcca tagctgcata tacggtggtc gcggtgtacg
     1861 gcacggatct cttctcggtc tcgggctggc aggagtcctt caggatcgta gggggcgatg
     1921 tctacggcgc ggcgaacacc atgtacgtcc agcccgtacc ctacgatctc gccccggggc
     1981 tcttcgtcgt gctcattccc ctggtcatga tcctggtggc cttcgcgacc tccgccaccc
     2041 tatacgagga gagccccgtt atctccgtgg ccgttctggg gctgaccata ggcgcactct
     2101 cgaccatcag cttcgaggat ggggcggggc ctttcttcgc cgttttcctg gtctgcgccg
     2161 tgggactctt tctctccacg ggtgtggccg gaccggatgg tcctggccgc ccggctgtcg
     2221 tggccggggc tatcgtggtc gcgctggtgc tcctcgtgcc gaggatgccc ttatccgacc
     2281 agacggtcag cgccggactt atagattgga cgcgcatagg gacgggcggc acctcaaggc
     2341 tcggagtaca ggccgatgtg ggagactacc ttacggccgg gagggaggcc gagctcctgc
     2401 gcatccgaag ttccgaaccg ctcctgtggc gcggcggaac gatggattac ttcgacggcg
     2461 tccggtggtc ggacaccacg cagccggcgg ccgacgacgg ggaggagata gcgccggatg
     2521 tcgagacccg tcccgtgctg cagagggtgc aggttctcaa cgcccagacg gacctcatct
     2581 tcggcgggta caagatagtg cgtacctccg agtatcgggc gagcccgaac tccgacggct
     2641 cctggtcgac ggccgaaccc ttcgaagagg gtgacttcta cgaggtgttc tcggagatcc
     2701 cgcagccaac cggggcacag ctcaggggcg cgggcagcgt ctacccgtcc tcggtggagg
     2761 agaagttcct ccaacttccc gaagacaccc cgcccgtggt cagcgagacg gcgcagaaga
     2821 tccaacgccg atacgatccc gcgacgcctt acgacagggc ccgcgcgatc gagcgatacc
     2881 tgatctacga cggcggcttt acctacaacc tcgatgtgag ctaccgcagg gcggacaagg
     2941 cggtagagga gttcctgggt gagggcaagg aaggtttctg tacccagttc tcgacctcta
     3001 tggcgctcct cttgcgcgac atgaacgtgc cctcgcgggt cgtctacggg tccacctcgg
     3061 gtgaagaggt gagccaggga gagtacctgg taaggggtag caacatgcac acctgggtag
     3121 aggcctattt ccccggtgtc ggctggtacc ccttcaaccc gacgccgggc ttctccatgc
     3181 cggacgcgat ggaggccaac gccccgcggc ccgagcttcc tgtggccagc gggatacagg
     3241 ccgccgagaa cagccaggac aggagccagc tcgcgcaaaa cagggcacag caacgggcca
     3301 gggagcagaa ccagaaccag gcggatcaag gtaccaccac ggaccgctcc gggcagatca
     3361 ctcttcctgt gtggcctctt ttcgtgctcg tgccccctct gatcgtggcg gtcgtaccgg
     3421 cctccaagag ggcactcctc tcccgcggca ggcccgagga cctctacagg gacctgacgg
     3481 gccggctgcg cgacgtgctg ccgccgaaca ggagcatcgt ggccgattcg cccgcgctga
     3541 cccccaccga gcgggtactt ctcctctccg gggccgcagg cgtggaggag gcgccgatgc
     3601 gccggttcgc cctggcctac tcggatcacc tctactccgc ggacggtgga agagatcaca
     3661 tagtctccgc ctaccgggac gctatgcgag cctacgagcg cttgccgcgt tggagaaggg
     3721 cgctcggagc cgtgaacccg gcctcgctgt tggccaggtc gaggaagtac gtgacggccc
     3781 agaaggccag actcggcaag atgctgcgtg gaagggctgg ccgtacttcg cgaaagcggc
     3841 gttgaagtat gcggttggcg gtgtaataat ttccttacta gatagcagac tacagcacgg
     3901 gaggtgctga tggaacagaa ggaagcaacc atcatcaaca tcactgacaa cgccgccgag
     3961 aaggtcaaga ccctcatggc ccaggaaggt gaggaagacc tcgcgctgcg tatcggcgtg
     4021 cggccaggag gctgctccgg tttccagtac agcatctact tcgacgacga ggtcgccgac
     4081 gacgacgagg tcttcgagac caaaggcgtc agggtgctcg tggacgccat gagcgtaccc
     4141 tacatcacgg gctctgagtt cgactacgtg gacggtctca tgggcgccgg tttcgccgtc
     4201 aacaacccca acgtccaggg cggctgcggc tgcggcagct cttttacctg ctagaccacc
     4261 gactcaaacg atcagcaatg agcccggagc atacagttcc gggctctttc cttgcctgca
     4321 gaacatctct gatcctgata ccaagtccgg aagaagagag gcatgtcagg tcgccacgag
     4381 atcgcttcgt atccgataaa gactccaaag gacgtggatt cccgcttgcg cgggaatcca
     4441 tcgcggcgag cgctccgctt atcctttcag caggatctac tggattacgt atgatgcctg
     4501 acttcaacgg caacgcttgg ccatgtagat atcgggtttg ccccatccgt tggcgtccgg
     4561 catcacgccg attatggagt tcctcggcgg ctccacgctc acgagtcttc ccgcagccgc
     4621 tccagggcga gcatgatggc cgcaacccca ctcgaactga ctatcttgcc cgagcggacg
     4681 aggttcaaga ggtcttccac gggcacgcga accacctcca cgagctcgtg ctcgtcggga
     4741 tcgggcgccg tgctctcccc gacgccgcgg gccaggaaga gataggcccg gttgtccttg
     4801 aggctggggg atgaggccac ggagcccaac tgttcccact cgccgcccgt atagcccgtc
     4861 tcctccgaga gctcgcggcg ggccgcttcc tccggtctct cgccctcttc gacgagccct
     4921 gcgggcaggc cgagctccat cgtttcgagc ggcggtcggt actggttgac gaggacgacc
     4981 tcgccgcccg cggtgagcgg gaagattatc gcggcgtcgg ggcgttcgat gacaaagtag
     5041 gggtctttga tcccaccatc agggagttgc agcctgtccg agcgtaacga gaagtacggg
     5101 gtctccatga gcctctcgct gcggagccgc ttccaccctc cgccctcagc caacgccggc
     5161 ctcgtcgatg gtgcccgggg tgcggccggc tagaatttgc ggaaggtctt tggtgaaagc
     5221 gctgcgggct acgacgcggt cacagccggc ctcccgagcg gcgacggcca gatctttctg
     5281 gacgtgggag aggaagccga ccgtagggac atcacgcgtc gcctcgtcgg acctcacgtc
     5341 ctcgaggagc gtaagcggct cgaatcgggc ggagttgagg tcgaggacga gcaggtccgg
     5401 tggggactcg tgcatggcct cgagcagtcg cctggggttg cgggggaaag tcgcctcgat
     5461 gccgagttgg gatgccgtct cggagatctt gcttctgaac ataaggtctt ctacggccgc
     5521 gaggatcctt ctggtcacat tgtcctccgt gctctagggt tgggtaagct cgcacattct
     5581 acccgcgggc ggtgcggtat cctatacggg tgaatagatt gaaggagctt cgcgccgggc
     5641 taaggcgcac agctgagacg ctctccaact ttacgccttt cttgaggcct cgccggggcg
     5701 ggcttgtact ggcgctcggc gtgttgctac tggagacgct gacgagtctt gcgcagccat
     5761 ggccgctcgc gctgaccatc gactacgtgc tgaccgacaa caagtcgctc cctgaggcgg
     5821 tgcccgctta cctggaggac aaggcagttc tgctcgccgg gatagcattc ctcatcgttt
     5881 tgatcttcac tgtaaccagg agcgtcgcct cgttccggcg ctacctgctc cagaagctcg
     5941 ggcaggagac cgtcttcgac atgcgtgagg ccctctacgc gaaggttcac gccctcgggc
     6001 tcgactacca cggcaagaag cgcacggggg acaccataac ccgcgtcacc agcgacgtga
     6061 aagaggtacg taccatgctt gtcgactcgg tggtcgaggt gttctcctcg gtcatgatcc
     6121 tgctcggtat gctggtcgta atgctctggc tcgactggca gctgactttg ctggccctcg
     6181 tcaccgtacc cttcctgttc ctcgccgtca aacgctacag gcaggcgctc gtgcagcgga
     6241 tgcgaatagt caggacgagg gaaggggcga tcgccagcgt catgcaggag gccataatcg
     6301 gtataaaggc cgtcaagctc ttcgcccgcg aggaagacga gatgggacgc ttcaggaagg
     6361 agagcaggga gtctctcaag gccagcgtgg acagcgcggt gatagaagcc aagttcagca
     6421 ccgtgctcgg gatcgtcgga ggtctcggca cggcgcttgt ggtctacttc ggggcgaggc
     6481 aggtactgtc cggcgccctc tctttgggcg accttaccgt cttcgtggcc taccttgccc
     6541 tgtttctctc ccctttgtgg gccctgagcc ggcaggtgaa ccagatcggc acatcactcg
     6601 tctcgggaga gcggatcata gagctactga acgcggagcc caccgtcaag gaccttcccg
     6661 gggctcgccc cgcgcccgcc ttcgacggcc gggttacctt cgagggcgta cgcttcgcct
     6721 atgagaacga ggccggagag gtcctgcgcg gcatggactt cgacgtcgaa gcggggagcc
     6781 gggtcgcgct ggtcggggtg agcggcgcgg gcaagaccac ggtcaccagc ctcatcgcac
     6841 gcctctacga cccgcaggca ggaagggtcc tcgtcgatgg tgaagacgta aaggagttca
     6901 ccctcaagtc cttgcgcgac tccgtgacct tcgtgccgca ggaccccctg ctcttcaggg
     6961 cgacggtcgc cgagaacata gcctacggcc ggccgggggc gagcagggag gagatcgagg
     7021 ccatagccga gctcgccggg gccgacagct ttatccggga gctgccggaa ggctacgaca
     7081 ccgttctctc cgagcgcggc gagagtctta gcggtgggca gaggcagcgc atctccatcg
     7141 cccgggccat gctcagggac acacctatcc tgatcctgga cgagccccag gccggcctcg
     7201 acgccgaggc cgccgccgcc gtcgaggaga gctggagggc cctgacagaa ggccgcacca
     7261 ccttcgtcat cgcccacgag ctcaggctgg tcaggagcgt agattcgatc ttcgtgatcg
     7321 aagacggcag cgtcgccgag gcgggtaccc acgaagacct cgtctcctcg gacggtctgt
     7381 acgcgaggct ctactccctg caagagcgcg atcccgtgca gccttagagg gggtgagccc
     7441 ttgagagagt tgctgatcct cagcgtggtc ctgacggccg tggcggtcgc gatctacatc
     7501 tctttcgcgt agcggctaat atgaggcgcc gcttgggatt ggtcgccttg ccactcatgg
     7561 attgggggta aagccctctg cggtgcatcc gcggccggag acccttcgcc caacttcctc
     7621 ctggtcaaac tctccctgta gtctaccgta agagactttg gaggcagaag atgctacgag
     7681 acgtttcgca taaaggtcgg ccttccgcaa gaacaagctg aaagctgacg gctgactgct
     7741 gacagctcgg atcgaggaag cccacttcct cgatccgcac tagtagagca ggcgtttgtc
     7801 aagcacgttg agcagctcct cgccggcgag gtaacggcgt aggttctcgc agaacagatc
     7861 agtctgggcg gcgttgatca gggccggcac gacatccgtc gtgtgggcgc tgacgatcac
     7921 gttgtcgagc tcccacaggg ggctctcctc gggcagaggc tcgacctcga acacgtcgag
     7981 cgccgccccc gagaggtggc ccccctgaag cgcctcgacg agcgcagcct catcgaccac
     8041 ggagcccctt cccacgttgg cgaagtacgc cccctgcttg atctcggcta tcgccccggc
     8101 gtcgagcaac cgccgcgtct cgggggtgcc tggaagcgtg acggcgacgt aatcagcctc
     8161 gccgagcgcg gcatgcaatt cggccgtcgg gtagagatcg tcggcgtagt cccaggcttc
     8221 gtcgtcctcg cgcaccgtgc gcttgacccc gattatgcgc ataccgaagg gtcgagcccg
     8281 agttgctatg gcacgaccga tactccccat tccgagcacg cagagcgtct tgttctccag
     8341 gctccccacc gccccctgcc gccagatctt ctctgtcttg tcgcgtctca aatggtcgag
     8401 ctccttggcg tactggagca tcgccatgag cacgaactcg gcgagcggcc ccgagtagac
     8461 gccgccggcc gtcgtcacca cgatatccgt ctcaccaagg ccggccttct gcgccacttc
     8521 tcccgctccc gccatgcttc cctgcaccca cctgagtctg ggggcgacct cgacgagatc
     8581 ctcgacgtgg ccgcggggga agtcgtagag gacttcggcc tccccgagca tcgccagaaa
     8641 ttcctcatcg ccctcgggcg ttctctccca ttcttcaggt ccagcatggt ctccgggcca
     8701 ccgcggaggc gggaccagat cctccctgta gaggacctgc aaccgctcgt ccacttcccg
     8761 tatgcgacgg acgtactctt cctcgaagaa actggttatg tggacggtgg tcacggtgcc
     8821 tccttcgtcg gcagcatgtc agcacgcgcc ctggcgggcg ctctcagcat ttcagcattt
     8881 cagccttcac atgcgcaagt cctggctgag ttgctgacaa ggcgcctgca tggcgccgtg
     8941 ctgactagct gaagtgctac tcttttcggt gtggacgagt tgctcgacat cctggacgag
     9001 tcagggttgc ctacagggga ggtcgttcca aaaggcgagg cgcaccggct tggtctgtgg
     9061 caccgttgtt ttcactgttg gatctgcggt tccgaccccg aagggtccta cctgctcctc
     9121 cagcggcgcg ccgccacgaa agatacctgg cccggctacc tcgacgttac cgccgcgggg
     9181 catcttgcct caggggagga gacggtagac ggtctgcggg aggtcgagga agaactcggc
     9241 ttgcggatag aacccgagag gctaatcccg ctcggaaccc gcagggtgga gcaggagata
     9301 cccgacggct gcgacaggga gcttcatgag gtcttcctcg tgtgcgacgc cacatctcca
     9361 ggggaccttc gtctccagaa ggaggaggtc gaggccgtgt ttcggctcga cctcgatgaa
     9421 gtgcaagcac tctacgagat gggttcggcg cccgcccgcg agtatgctga aggcaggatc
     9481 tctgcaacgc ggatacacct cagcgagttc gtcccgaaag aagaaggtta cctgcggagg
     9541 gtggccgggg cggcacgtca gctcctgtcg gacacaccgc cagaccctat cttctagggc
     9601 gattcgcaaa gcatcgacct gctacgaatc acgagttagg ttacgggaga gagcaacatg
     9661 ctgacaagcc gcaggtggcc tggccgatgt acactcttca agcacgaact ccggcaggca
     9721 cgaacgcatc aggcgcgata tatttagtgc atgagggttg cgctggccca gatcaacacc
     9781 accgtagggg atatctgggg gaacgtcgag aagatggccg acaccctgga gcgggcgacg
     9841 gattcgggcg ctgagctcgt tgcatacccc gagctgacca tccccggcta tccgcccgag
     9901 gaccttctca tgcgccagag ctttatcgag gagaacgtga gggcgctcca cgagttcgcc
     9961 ggacgggtac ccgagaacgt ggtcgccgcc gcgggcttcg tcgacctgga cgccgacctg
    10021 tacaacgcct gcgcccttct atccgggggc ggggtactgt accgctacca caagcgctac
    10081 ctgccgaact acagcgtctt cgacgagaat cgctacttca gggagggatc gggagctccg
    10141 atcctcggtc tgggcgaagc cctcgtcggg gtgagcgtgt gcgaggacat ctggtaccct
    10201 ggcggaccgg cccgcgagca ggccctcggc ggggctagcg tcctcctcaa catctcggcc
    10261 tccccctacc acaggcggaa aggcgccgcc agggagcgga tgttgagcgt gcgggcttcg
    10321 gattacggct gctacgtgct cttctgcaac ctcacgggcg gccaggacga gctcgtcttc
    10381 gacggccact cggtcgtctt cgaccccgag ggtaggctcg ttgcgcgggc cggccagttc
    10441 agggaagacc tgctgctcgt cgacctgtac ccggatcact cgctcatgca acgcctccac
    10501 gatcccaggc cgcgcaagga gaacgccgag cataaacccg aggtcgtacg agttccccga
    10561 cacgagacgc ccgccgtgca accggtcgaa cccaggatcg aaccttccct accggaggag
    10621 ggcgaggtac tcgaagccct cgtgctgggc ctcgcggact acttccgcaa gaacgggttc
    10681 tctcgggccg tgctcggtct ctcgggcggt atagactcgt ctctggccgc cgccctcgcc
    10741 gtcgaggccc tgggtccccg gaacataacg ggtgtcctca tgccgagccg ctacacctcg
    10801 gaagggagca ataccgacgc cgcggcgctc gccaagaacc tcggcataga cacccagacc
    10861 atacctatag gtccggcctt cgacgcctac agggagatga tggagggtgc cttcaagggg
    10921 ctccccgagg acgtgacgga ggagaacctg cagagccgca tcaggggcaa catcctgatg
    10981 gccctctcca acaagttcgg ttggatcgtc ctctctacgg gcaacaagag tgagatgagc
    11041 gtgggctact ccaccctcta cggcgactcc gctggtggtt tcgcggtcat aaaggatgtg
    11101 ccgaagaacc tcgtctacag ggtggcgcgg cacttcaacg agaggaagaa ccgcgagatc
    11161 atccccgatt cggtcctcac caaggagccc acagccgagc tgcgcgagaa ccagcgcgac
    11221 acggactccc tgccgcccta cgaggttctg gatcccatcc tcgaagccta catagaggat
    11281 gacaggggtg tggcagagat cgtggcctcg ggattcgacg aggacgatgt gcgtcgcatc
    11341 gtgcaactcg tggacagggc cgagtacaag cgtcgccagg ccccgacggg cataaaggtc
    11401 acagaaaggg ccttcggccg tgacaggcgt atgcccatca ccaaccgcta cttcgaacgc
    11461 aaccacaaag aatagccgta gaggtaaggc gagaacgccg gggattcggc cggcgcgccc
    11521 actccttgca gaccgcggga gaacccggct acgagtcggc ggtgtaccgg cgcttgagga
    11581 accttacctg caccgcagta ccgcgtcccg gggtggagat tacatcgaga tcggcgccga
    11641 cctctccggc acgccggcgc atgtactcca ggccgaggcc cggtgggggt tctctcctgt
    11701 cgaagccgcg gccgtcgtcc cgaacgcgga cgatcagcac tgggccaacc cgacgggact
    11761 ccaggtacat gttgcccgcg cccgagtgct tggcgacgtt ccagaatgcc tccacgaata
    11821 ccctgataag ggcggcggtc tcggccgggt tcaatacctc cagagaggcc tggatgtcgt
    11881 cgtgggtctt caccccgaag tactcctcga acttccgcag gcgttggcgg aggaagtctg
    11941 cggccccggg cggactctcg ccgcgcaggg cagatagctc gtcgtaggga cgggagatcc
    12001 tgaactcggc ctccctcgac gccgccaaga ccctcccgaa catctccagc gcctcctcac
    12061 ggtctccacg gcgctcggtg tcgagggcgg cctgcagcgt cagcgagatg ccgtgcactt
    12121 cctgcttcgc cgtgtcgtgt aactcgtaga gaaggcggcc ctgctccttt atacgggccg
    12181 attcctcccg ctcctggccg aggtgtttgc tgaccgacga gaccagtccg atcctcaaag
    12241 ccaagtacag caggagcacg gcacatcccg cgagcacgta agccggcagc ggcgggttca
    12301 tggcgcccca gacgagtagg ataatgaagt gcatgggcgg ggagagcgga ccgagccagt
    12361 acaaggctat ccgccacggc tgtatccgcc cgacgttcga gtcggcagga acctcagggg
    12421 aggtcgtcga cgtgagaccg agcaggatca tgcccagagc ccacaacatc tccggccagt
    12481 tgccgctctg atacggcccg acggagcgaa ggtccaggta agcggcatca gcgaggagca
    12541 gcaccacgaa acccccgttc agaaacgtag agaaacggag cctgatcgag gccgaggcga
    12601 ccacgagccc gaggaagagc agggccgcgt cgcagacagg ctgggagagg gcggccgcgg
    12661 cttcgcgcac actccctagc ccggcctcgg tcgctgcggg accgaggacg aagtaccaga
    12721 ccagcgtccc gaccgagagc atgacggctc ccgcgtccag ggcgctgacc agcgtgatcc
    12781 tgggcgtggt cagcgccacg agatggagca tcgctcctac cagcaacgcg tatgagatgg
    12841 cgtaggcaac gtcctgcggt gccaccggcg tgacgaaccc aagtcccaaa tggtgcgacg
    12901 cgctccagag ggagttcccg gcgagccggc agaccatgcc aagtcccatg agccgcagga
    12961 aggcctgctc gtggccatcc gcgcgcggtg cggcgcgcag caggatcccg gcggcgaagg
    13021 cggctgtaga aagggagaaa gccgtggcga ttacggattt tgcctccgag ccctggggca
    13081 gaaacgcgaa gacgagcgcg cccagagccc agcaggccag aacggccgtc ggaggaatga
    13141 gtggaacgcg cggatcgcgc aacttacccg catccgaacc ttgcaactct cagccctcca
    13201 gaactcgaac gcacccatcg taccaccctc gcgccttgcg tggccagtga ggtcggccga
    13261 ggctcgtatg ctagagtacg gcctttcggt ggcagagatc cgagaggagc agcgtatgga
    13321 gtacgcacgt cttgggaaca cggggctgat ggtctccgag ctttgcctga ggtgcatgac
    13381 gttcggtcag gaggccgacg agaagacctc taaagagatc gtcgccacgg acttcggact
    13441 gcgcgctgtt atgggctacc tgccggagag aagctagctc aggcccggtc cgagacgcct
    13501 tccgcgccgg ccatacgcgc gcagtgggcg cagcagtaga aagagccgcc ctcctcggtg
    13561 ccgtgcccga tcaccttgac gttgcagtac tcgcagaccg gggcgagggc gtggatggca
    13621 cactcgaagc tgtcgaaggt gtgcgagtct ccatcggcca cgctcacctg aaaggcctta
    13681 tcgtactcgt ttccgcagac ctcgcactgg gccatatcta tctccccttc tcggagcaga
    13741 acgattccct gtcctgagaa cagttacccg ggaccgaacc gggacaaacg cccctaacat
    13801 ccacgcactc gggtgaatag gcccctcatg cgttaggtgg tgtgctcgaa gcgactagag
    13861 taaccgcaaa atcactcact tgagggttgt gctgcacccg ttcagagatg tagggtggcc
    13921 tcatacgcta atggagcggg tcgcccgccc tgctccggga agaggaggtt gcgcatgaag
    13981 cgtattatca cgttgtcgac catagccgcc ctcatgttgg gtgtcctggc ggctggggtg
    14041 gtatatgcaa acaacgagga cgctggggcg aagaagtgct ccgtggccac gcttcacggg
    14101 acgtatctgt tcgcctttga tggggttgaa atcaagggga atgatcagag accgttcgcc
    14161 attgcagggt atgctgtgtt tgacggcaac ggtaaggaga aaggcgtcgc ttcctctaac
    14221 tctaacggag aggttactcg taacgagcgc gtctccgcca cgtacaccgt caaggcggat
    14281 tgcacaggca ccgtcacctt tggggatggt tcgcagatcg acatgttcat cgcccccgat
    14341 ggcagtcagt ttgcgttcgt ccagaccaag ccctccaagc ttgtggggtc ggggttcgaa
    14401 ccgcggggga cggccaagcg ggtcggagac tgactcacag cgggtagcag aaggttcaac
    14461 cttctgctac ccgcgccgtc agcactaagc tttcagcttt ttgttcttgg ctgaaagctt
    14521 atagctgata gctcgtcgtg cataagggtt caacccttat gcacggcgct gtcagacatc
    14581 tgatagtcca ctcgaggaat gccctcgtat atacgggagg cgcctattcg gggctcggga
    14641 aaggaagtat aagattgttg ggacaatcgc cgagccccga attcagaggc ggacctcccg
    14701 gatctctata cctcttcagt acgatccgga aacataagca aatcggttac agttgggacg
    14761 atcctgacga cggaacgggc gaccatactt cctggaaaac acgttccatt ttgtgagtga
    14821 gggcaaagag gaaagtccgg gactccgtag tggcccccag ccccgcacac tgcgctaccg
    14881 ctcggggcta aggatctaac cctagtgcgt cgaacgcctc ctctaccgag cgcacctcat
    14941 cctccgagag ccgcccgctt gtcctggagg gatgttcatc ccacacggcg taggagaaca
    15001 ccgtgttcca ggccgcttcg ctaagcgcga ttccgcgttc ggatagcacc ctctttatgt
    15061 gaccctcctg ttcttggagt atcgtgtctg tctctttgtc tgcctcctct tcgctgtggt
    15121 gaaactcctc gaccctgacg atgtacagct tagggacccc caggaggatc ccggtggaga
    15181 tcaggaaggc actgtccgga tcctcctcgg cctcgctcgg cgtggggaag acttcgaact
    15241 caccaacctc cttcgccatg gcgaacaact cgttccgaac ggagctcttg tccatctctc
    15301 tcctcatgaa tagagggccg ggataccctg aaggataccc cggccattat tcttgccccg
    15361 gcacttacag ttggcccaac atctcaaaga ggccgacttc gtaccgctct acatcctcta
    15421 tcgcgtcgcc ggtgacctcc gctgtctcgc tgcgcgtacg aactccgact tcctcgctgg
    15481 cctgcatggc ttgctcactc tcccaaaagg tgatgccgat cagcttgccg ctctggcggt
    15541 cctcgagggt gatgaaccct ttgaaaccgt cctgttgctg caacaggggc aggacgtgct
    15601 ccctgatctc ccgcagtact tcatctattt cgtgtgggga tccctccaat gtactcatcc
    15661 tcgcgtgcaa ggtcgcatac ctcctttctc ccgcctacag ggcctgtcgg acgatcccat
    15721 accactgtaa gcataatgcc accgcaagcc ccacgcgaac ctcaaatggg gaagggctta
    15781 gccccaccct aaaatgtaca ggcgaaggtg gtgaatgata ctatcccgcc agttttgggc
    15841 tctagccaag cattggagat ccggggaccc gtcctttgtc cctagggcct tgacgcttcc
    15901 gctatcatga ggcgtccgag aggtggaggt gaacggtagg aggatcatga acgtggaggc
    15961 ggcgatggtc gtgagggcgg tgggtggtgc gctgctcatc gccattgtcg cggccgttat
    16021 cgttaccctt gttagccttg ccatgttcct ccgagagccg atctatgagc cgaccgcccg
    16081 ggtgtgggtg gaaagagacc tgagggttgg gccacccgct ccagggccgg acacgaggct
    16141 aagcgagact accccggcga tggtccgcct ggcggccagt cgccccgtgg ccaatgagac
    16201 caggcaaagc ctcgggctgc aagcgagttc cgctgagcta ctggataacc tggaaatacg
    16261 tcgggtcccg gacacgacct tcatggacct aacataccgg ggtgccgacc gaaagatggc
    16321 cgcccagacg gtcaacacct tcgccagggt cgcgtccgaa cgcctctctg atgcggtcgg
    16381 caagaacctc tcggccgtcg tctgggagga ggcccgagtg cccccggccg tgcctgagcc
    16441 caaaccccta agaaacgggc tgctaacgct ggttggggtg tgggcgctgt acgcggggct
    16501 cacccttgcc acgctggtcg tcttgaggcg gtagcctgga agtggagcag atcctcgagc
    16561 cgcggcacct ctgctgtcag ttatgagccg tcctctacga cgactattcc ataccgccgc
    16621 tgagccaaac cctgtgggag ttgcctgaag agtacaccgc ccctaccgca gacgggccat
    16681 tatgtgggcg tccacgtatt cgccgttgcg ataggcgaac cgtctgtggg ttccctctat
    16741 ctcgaacccg aacttctcgt agagggctat ggcgggctca ttatccgtgt acacgcgaag
    16801 gtccaacctc gtaagcttca accagttgtc tgcgaggtcg aggcaagcct ccatcaatgc
    16861 cgtgccgatg cccctaccc
//
LOCUS       DATMVF010000089        31053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_606224, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000089 DATMVF010000000
VERSION     DATMVF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 31053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31053
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606224"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VN178_03985"
     CDS             <1..567
                     /locus_tag="VN178_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80161.1"
                     /translation="IEVNEETLAQSLAILDGEGRVIGAAFNETMAPLDVEPPLRKDDP
                     FLDAVISVWAPVYGALGAQDATALAALSERYPAFREAYEKGKVSHHILIARSDELPKE
                     DTFELVAAGGERCRTLGYEYVVTEATNQWTGAAFEALGGVRVHFAPFLVQQTVPKSDE
                     PLEDVLTSPNGFLAENDSGGMFYVIRLA"
     gene            710..1540
                     /locus_tag="VN178_03990"
     CDS             710..1540
                     /locus_tag="VN178_03990"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS80162.1"
                     /translation="MGKGNTMHETNDVEFATTSVATDVTLHYAERGDREGKAIIFLHG
                     YSDSWYSFSRVLPLLSPSYHAFALTQRGHGDSDKPECCYTPHDFATDVDAFMDALGID
                     IATIVGASTGAVFAQRVALSYPRRVCRLVLIAPQTPAHENEALSGLREEVRTLEDPVP
                     PEFVREFQEGTIHHPVPQEFLETAISESLKLPAHVWRDYLDQAVLSIDRDYVVELGEI
                     DVPTLILWGERDPLFPREEQERLAAAIPGATLKVSTLKVATRRTGNDRSGLYGILKRL
                     "
     gene            1631..2932
                     /locus_tag="VN178_03995"
     CDS             1631..2932
                     /locus_tag="VN178_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80163.1"
                     /translation="METRAHPPGLQDVLEFPLVEALFGRRARRFSLGASLPDGPLEFT
                     SRHDPLPLSELEQMLVLTAAAGNTGWHYMIMRHARYAPHLSNYSGAAGGRTFPSAAGF
                     HTSELFFTDDEGVYLFETRDAPALVERTTDGPPDLDAILDAHRGRIRKLSDGRLYIPA
                     EEPYMEGHNTWCANRPGSTLLIPVGDIAQHMIAVLCFMVQNGYGMYDDVNGERIPGLE
                     RFSHLVDLEEPLPLTFMETYSITECTAELSTSCYAGMLMLQAMGLGGWMFDGIDRYTV
                     LGASGDPDVPGLGFRYDTDERWPLPNPTGLPGVFAGYCPPHYPDMRAAVEAFAERKFG
                     PGGPFNPDTPGPWKESTRVRSSAQVHSEEFKECVTVMAQYIYDRFGKFPGTVPSIFIL
                     TYLQAHHLELEFYDHHFGPGAYLRTHADHMARWHGRGEEEV"
     gene            complement(3133..4050)
                     /locus_tag="VN178_04000"
     CDS             complement(3133..4050)
                     /locus_tag="VN178_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWS80164.1"
                     /translation="MRVLLTGGAGFIGSYIAEHLLQSGHEVAVVDDLSTGRRENVPRG
                     ARIYEADVRSGCTEIFRDFGPDALCHQAAQMDVRRSVREPDFDAEVNVLGTVRLLENC
                     TEYGVHKVIFASTGGAVYGEQHQFPAPEDHPQYPVAPYGVSKLAGERYLHYYQVQFAL
                     PYVALRYANVYGPRQDPHGEAGVVAIFCGNLARGTTSTINGTGEQTRDYVYVKDVARA
                     NVLALESDVPSGAYNIGTGAETSVNRLYDLLLEISAKDLPPRHGPSKPGEQLRSCVDS
                     TLAGRTFDWHAQADIRAGLEETLRSFDVP"
     gene            complement(4052..5059)
                     /locus_tag="VN178_04005"
     CDS             complement(4052..5059)
                     /locus_tag="VN178_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HWS80165.1"
                     /translation="MTTSRPAARRPRALVTGGAGFIGSHLCERLLAEGYKVVCVDNLS
                     TGSLANVAPFLANEADFEYVEHDVNTHMDVAGGLEEVYHFASAASPADFGRIPIEILK
                     TGAQGTHNCLGLARARGARFMLASTSEVYGDPLVHPQHEDYRGNVSTTGPRSVYDEVK
                     RYAEALTMAYHRHHRLDTRIVRIFNTHGPKMREDDGRMVPNFIRQALLGEPLTVYGDG
                     TQTRSVQYVDDLIEGVIRLMKSGERRPVNIGNPVEYSVREVAEMIVELSGSGSDISHQ
                     PLPEDDPKQRCPDITRAREVLGWEPRTGAREGLAKTLDWFASQAKTRSRASTTTRGEG
                     S"
     gene            complement(5170..6360)
                     /locus_tag="VN178_04010"
     CDS             complement(5170..6360)
                     /locus_tag="VN178_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80166.1"
                     /translation="MTKAVGQWTSQIVEHTRRLEEYKKTISRLFPALPKDLLDAASIK
                     DVDALVLALFLRCYPHELSVLEVGTSLGIATFHFAGQPSVSRVLGVDPSATEDTAETS
                     SIDIARAALAEFADESVKIKLRPGDLSGASAKLAHELPENEPLLAYVGGARTREAVDA
                     DLQAIFDANPRAFAILDHCRGEEGPFVQAGIADCMQRAPGKYHFRLFGDLSSGMATSK
                     LGIVYPDEESSEVQRSLTELAHLFSERLDPLWLAGREQELIGIVSAYRDEADTLSGQL
                     QDLLGEHQSLAGRNTELQKRISQLERRKSRLEERNAGLKIENKGLQSQTSRLEKRILQ
                     LEKRASQLEERNSRLKEERLLVISKLSDYRSAGRYKLADALAKNVLRIPGVKAVVRRT
                     RPKE"
     gene            complement(6385..7278)
                     /locus_tag="VN178_04015"
     CDS             complement(6385..7278)
                     /locus_tag="VN178_04015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWS80167.1"
                     /translation="MSEAKILQSTNRIESTVLADPGSESLYGQSKEENGVTIFIPNWN
                     HRPYLPRSIRSALRAIERLEEAGHSGELLVIDDASRDGSQKFLRTVQVLYGDAPLRTV
                     FLERNLGLPRLRNLAMRISRYRHVCMLDADNELVADNLPLFLQSSVRTGAALVHGLLI
                     DKREGKMTGIRSDNVASMRLTAGNYIDAFALLDTMKVLGAGGFVTDQRLYGYEDWEMI
                     LHLIAEEEELVFVPVALGYYHLTPGSMYAETAEDKGPPPRSAAPEVVTLLRRMFAQTG
                     TREWDAVRVGRIYHPAVGYID"
     gene            complement(7271..8182)
                     /locus_tag="VN178_04020"
     CDS             complement(7271..8182)
                     /locus_tag="VN178_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80168.1"
                     /translation="MQTETLAIVRPPTTGDQNLASRLSKGFRDASHRVAVYSSPEVSA
                     LAPDDLNNTTLVVVSPGQCIETSGNEGAFLSKVARARKRILASVGPVHSQGYLSRLRR
                     GIHFDALFDLGFVSQRDSHYKVTNLAYHFVFNGLTREEEPLAEEPANRAERTIPWVLV
                     GPRSDTNRDLLGALFEHQVDPGGFCLLHARQKNTTPPEPTLGGPQLSAILSKARYYLW
                     GAEREGMYYESFRFIVALLAGTVPCKIDPALAAVGFDVPGVYASVSAFDTEVRDMGYS
                     EMYRRASDFYVFRGRLGEHLSGALRLV"
     gene            8362..9603
                     /locus_tag="VN178_04025"
     CDS             8362..9603
                     /locus_tag="VN178_04025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWS80169.1"
                     /translation="MNENTGAARDMGLTRPYSGARSQGGKVSVVIPCYNQARFLGEAI
                     RSVLCQGYADLEIVVVDDGSSDGAAEVASGYARVDARIRLIRQENRGLAAARNRGLAG
                     VGGDYVVFLDSDDRLVSGALEVGVRELEEHPGCAFVSGLCRKIAADGSVVPGWEQFRV
                     RDDPYLELLRSCPIYVPAVMYRRCVFDAVGDFDTSYKAAEDYDLYYRILERFPVYCHD
                     TIVAEIRRHDANMTRDRTLMLKYNMAALRSQRKRVKGDARYKAAYKAGERLWRDWHGA
                     PVVNQARSHLREGRWREGLQGMLVLLRYYPRGILLVANWGVQRERLARRLRHQQRELE
                     THRYKLQQLKVPGETPAQNGDRRDKELRIAIKEERQKIRHHKQRIRRLTSRMQRRKQR
                     TDALRKGLQSVKARLRLTSRG"
     gene            complement(10424..10753)
                     /locus_tag="VN178_04030"
     CDS             complement(10424..10753)
                     /locus_tag="VN178_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80170.1"
                     /translation="MPLFRELVEPLGVPLRDDGQVRFVGGFYAQWQWMVTNLKYVSVP
                     FLQRMLREEVGEKWTEFSCHPGYPSPDFAAVYDAEREAEVRTLTDPRIRQTADGTGHP
                     LGELCRL"
     gene            complement(10753..11094)
                     /locus_tag="VN178_04035"
     CDS             complement(10753..11094)
                     /locus_tag="VN178_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWS80171.1"
                     /translation="MEKCLIVNADDFGASTGVNRGILECHTRGIVTSASLMVTGRAVG
                     EAVSMSLDHPELAVGLHWDVWGEDEREFDIDNLQAVHVNFHEQLDEFYRLLGRMPTHV
                     DSHRHAHRGGG"
     gene            complement(11191..11715)
                     /locus_tag="VN178_04040"
     CDS             complement(11191..11715)
                     /locus_tag="VN178_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80172.1"
                     /translation="MEYNGEVYRWSKHHEWEKFLGLPRCTKQPFELAMNVDDWASRER
                     LEAHGWALVSPLQMSLDVFGDYPAYFRRSRAEFTVAKDQNVRLRSGWFSERGACYLAC
                     GKPVVAQDTGFGNVLPTGEGLFAFTTMGEALGAIEAINGDYRRHCEATRTIAEEYFEA
                     RSVAARLLSNIGLA"
     gene            12346..13482
                     /locus_tag="VN178_04045"
     CDS             12346..13482
                     /locus_tag="VN178_04045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80173.1"
                     /translation="MRVEQLRGIEDVDAETWRTLEPPEFPFFDFEFLRALERSGSIGR
                     RSGWIPVYLVCKEGARVLGALCLYLKTDSYGEYIFDWEWARAYQQYGLSYYPKLVAAV
                     PFTPATGPKLLLRRDIDVATRSRVARALLEAAEDLGTEYGVSSSHALYLPEHELGEFK
                     RRGFAVRHSLQFHWRNRGYETFSDYLGALSGKRRRQILRERRQLEELEISHLTGDALL
                     PEHAASMYEFYLATLDGKWGVPYLNRAFFDEVFRTMRDRTLLILARGRADRPVAGALF
                     FLKDSALFGRYWGAAEHRRNLHFELCYYQAIDFAIERGFELLEAGAQGEHKLARGFLP
                     SLTYSAHKIRDPAFGRAIGEYIESEKQMLQNVMEEYASHDPYRR"
     gene            complement(13492..14454)
                     /locus_tag="VN178_04050"
     CDS             complement(13492..14454)
                     /locus_tag="VN178_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWS80174.1"
                     /translation="MTHKTNLPQPLDETTPSLHQLIPEADRLVALYLRTLTSPQTIKT
                     YNTELSLFISFVECEVGKGLGELTAEDISLYREHLLETYATATAAKKLAVLRRFLIFT
                     YMAGATTVNPEALRFFAKSPRVRQDPAYNVLTEDELSRMLNAARASNYRDYVMLAVMA
                     GCGLREAEIVGLRIGDFREAVADQVLLRVLGKGDKVRNVPISPDLWRLVQRFVLLTER
                     SFTSHPDSRKPLFTSRVGKDKPLTTRSVQNIVKKYVRAAGITKAISPHSIRHTVGTNM
                     AVNEAPLLVIQQFLGHSDPKTTMRYIRRAEELATRAYQYNTLPL"
     gene            complement(14438..15001)
                     /locus_tag="VN178_04055"
     CDS             complement(14438..15001)
                     /locus_tag="VN178_04055"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWS80175.1"
                     /translation="MSGPAQGTQDAARALRKHIHTVPDFPRQGVSFKDITPLIEDGHA
                     LHTAVDALAESTEGIAYDRILSAEARGFVFGTALAYRAKKGLILARKPKKLPREIISA
                     TYDLEYGTDALEAHADSIPEGTRVLVADDLLATGGTARAMCQLVENAGGVVAGVAFLI
                     ELAYLGGREKLEPYPVVSLITYHDPQD"
     gene            complement(14998..15735)
                     /locus_tag="VN178_04060"
     CDS             complement(14998..15735)
                     /locus_tag="VN178_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWS80176.1"
                     /translation="MKSVENDGMDREINGGYTTEGYAQERYSATKDSFTIGEVVERLR
                     PEFPALSVSKIRYLERRRLINLSRTRGGYRLFSSQDIELLRYILTLQDKEYLPLKVIK
                     KRIEGGGGFSAADSAGDLSRVLEERTYTREELADALETEMRFVEELISVGVIGRSGEL
                     TQRDAEIARMALEMAKYSIQPRHLRGIMAAVDREAALFKQVLNPDLRSGDDGRVGEAM
                     NKARHLAAVDSRIKELMMANVLDTFSP"
     gene            complement(15767..16216)
                     /locus_tag="VN178_04065"
     CDS             complement(15767..16216)
                     /locus_tag="VN178_04065"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWS80177.1"
                     /translation="MKECPGCGAEVSAGMKFCVECGTRLDLGQSTVSYAPSFGEDVEP
                     GTSGGAEGAALIEMDQVEGTAGRRMHDLGDDVVSVGRGQESSIFLDDVTVSRKHAEIV
                     HGDGGYRIRDVGSLNGTYVNRVRVDAVDLRNGDEIQVGKYRFRFVFS"
     gene            complement(16213..16470)
                     /locus_tag="VN178_04070"
     CDS             complement(16213..16470)
                     /locus_tag="VN178_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80178.1"
                     /translation="MDDSGLHGGFDWENAEDVTALSEEDLRDTLAALSEEERRLAYRR
                     AILRGRMDLIRAELVQRGAAALPPGRLARVLLGDSPVEETR"
     gene            complement(16509..19025)
                     /locus_tag="VN178_04075"
     CDS             complement(16509..19025)
                     /locus_tag="VN178_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWS80179.1"
                     /translation="MKAIIMAGGQGTRLRPLTSDQPKPMIPIVNAPCMEHIVKLLERH
                     GFTDIAVTLQFLPDEIRDYFGDGSDWNVNMRYSVEDAPAGTAGSVKMAEQQLGLEGER
                     LLIISGDALTDADLSTLVEFHEEKGSEATMVLKSVENPLDFGIVITEEDGRISRFLEK
                     PAWGQVFSDTVNTGIYLLESSAIAEIPDPEEGEYDFSKELFPKLLEAGRPLYGYITED
                     YWEDIGTLEQFASAQRDVLDGHVEGVRPPGTRLRENIYVGRRAQVDDDELEGPVVIGE
                     NTRIHEGARIGPYSVLADNVVVSAGATIERSVIAEGTYIGEGAELVDTLVGRNSYVQA
                     RARILERSALGDDVIVGEGATIAPEVKVYPHKTIESGANVTQSLIYETMGLRTVFRRG
                     VVSGKFNVDLTPEFVTRLASSFGTTLDPGAVVTLGRDSSLSAQVAKRAMTAALLGTGI
                     NIRDLRAAHAGVVRHDVLAGKSSAGAHVRAGSDPDDVEILFFSADATPITEGDERSVE
                     KAFVREEYRRAYGGDIGELIYPGRVLEQFVERLERVVDRNRTSGATIVVDFFGGVAGL
                     VASRVFSRLGVSAVVMEGFANANAVGEAGGLNLEESIERVARIVPTVDAAFGAVVGPE
                     AEYVQFVDDEGEWVPHDVMLACLIDQMRPKSAVLPINLSRAYERLVKEGGGAVEMSRT
                     GLSNVAIKAADIGADLASLADGRYIFPAFLPSPDCFMTLASALEVFRETPLSGVRRKF
                     GDSFDHVQREHIECPWSAKGRVMRGLAEKFGGDPDAILAEGVLLNVEDGWVLMLPDPD
                     SPMFYVYAESGEGDGSPARLMGEYVDLVKSLIDDQALLSS"
     gene            complement(19383..20687)
                     /gene="der"
                     /locus_tag="VN178_04080"
     CDS             complement(19383..20687)
                     /gene="der"
                     /locus_tag="VN178_04080"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWS80180.1"
                     /translation="MSRLPVVAVVGAPNVGKSSLVNRITRRRGAVVSERPGTTRDRAY
                     NRAEWAGREFTLVDTGGMEPRARVGLEALVTLQARVALEEADVVVHLTDARLGPTEAD
                     AVIARELRRARGKAVLAVNKLDNPADDTERHRFYSLGEGEPHAISAVHGLGVGDLLDV
                     VVSLLPESQPEGGVEPPALAIVGRPNVGKSTLLNRLLGTDRAVVSEEAGTTTDAVAAE
                     IALDVEGEPERYLLLDTAGVSKGARRARGVPYYSSLRTSEAIRRSDVSLLLVDATDGI
                     VAGDLRIAREIEEAGSSCGVVVNKRDLVPPQRLREIEGLISARLTDLEPPFVHISALT
                     GAGVDEVLPLAAGVARAYRLRVPTHEVAEFVGDVVARTAPPKGVKLRYATQTGAAPPR
                     FTLFATRPKDLPENYLRYVENALRERYGLWGVSVRIKVKSSR"
     gene            complement(20684..21337)
                     /locus_tag="VN178_04085"
     CDS             complement(20684..21337)
                     /locus_tag="VN178_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWS80181.1"
                     /translation="MRRAMLFEGRRKPGEMTRRYRLFRRAVTLLCRLLFACRVHGGER
                     VPSEGPLIVASNHSQYADPVLVCVAVPRRLQWMAKKQLFVFPFRRFFEFIGSFPVDRE
                     GGGRAAIRAALAFLAEGWALGIFPEGTHRGAGATREAKTGVVVLALRSGARVLPVHVG
                     RLPGPLTRLRGGRLEIFVGEPVHLDPAIRGGAAYRAAADEIMHTIYALPQRRAQGSL"
     gene            complement(21334..21999)
                     /gene="cmk"
                     /locus_tag="VN178_04090"
     CDS             complement(21334..21999)
                     /gene="cmk"
                     /locus_tag="VN178_04090"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWS80182.1"
                     /translation="MGGILVAIDGPAGSGKSSVARAVAGRLGVVNLNTGAAYRAAALL
                     ALREGVDLTDAEGLAALARRVALDPEGASIDGGRVRERDLRSPEVSAAASKVSAHAPL
                     REVLLPVQRAAAGKAKARGGAVVEGRDIGTVVLPNAELKVYLSAAPEERARRRASQTG
                     REGELDRIRAAIARRDRQDSEREISPLKPAADAVVLDTTSLSLEEVIAKVLDLATDLR
                     GRT"
     gene            complement(22034..23362)
                     /gene="aroA"
                     /locus_tag="VN178_04095"
     CDS             complement(22034..23362)
                     /gene="aroA"
                     /locus_tag="VN178_04095"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWS80183.1"
                     /translation="MKNLAGVPGVDFTVEDVRPTLPAQLEVTPLKDPPNATIRVPGSK
                     SVTNRALIIAALAAGSSRILNPLFSDDSFWLMNALVRLGIGVSAEREKGEVYVSGQGG
                     GFDASGVDLFVGNAGTVARFLPPVLALGRGPYTVDGVPRMRERPVAGLVDAMRQLGAA
                     VAYAGEDGKFPLTIKGGGLRGGEARFSASKSSQFISGLLMAAPYAEAPVKLYPEGRKE
                     WPYVGITAALMRAFGVEVVETNGRFAVDPATYRSREYEVEPDASGASYFMAAAAATGG
                     RVRIPGLGSSSPQGDLRFAEVLRDMGCRVEITASATQVTGPDRLRGVEVDMNAFSDTM
                     ITLSAIAPFAQDPTTIKNVAHTRLQETDRLRAVQTELNRLGIKTRTTPSSIRIIPGKI
                     RPGVVRTYGDHRMAMAFAVTGLVAPGIAIGDPGCVSKTFPGYFGALRSLG"
     gene            complement(23359..24054)
                     /locus_tag="VN178_04100"
     CDS             complement(23359..24054)
                     /locus_tag="VN178_04100"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWS80184.1"
                     /translation="MRLQAFLARAGAAPSRRKAEAPILAGRVTLNGETATLGARVTPG
                     DEIELDGHPVRLPEACVYLALNKPTGYLTTMNDDRGRPTVAHLMPEVPGLVPVGRLDA
                     TTTGLLILTNDGGLAHRITHPSSEIEKRYRLTLENPAPTEAIEQLAAGPTLDDGKMHP
                     PEITNLSRSRETTTLDLTIHEGRNRIIRRACMAVGLALISLHRTRVGPVALGDLPEGE
                     YRALSPSELEALR"
     gene            complement(24054..24662)
                     /locus_tag="VN178_04105"
     CDS             complement(24054..24662)
                     /locus_tag="VN178_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWS80185.1"
                     /translation="MIDPQRKLPSPAHLVEAVLLVSASPLSASDLRSATGLSDGELKS
                     ALARLRERYAPEASGIVLRDVAGGHLLSTNPACSAAVERFRREARPAPLSGAAHEVLA
                     CALYLGPLSRGAVSRVRGVNSDAVVRSLIERGLLAEVGTDREAPGWPALLGVTEEFLS
                     ATGASSPDDFPPAGSLVTQEELSRVRERLASSGTGQPDAGEP"
     gene            complement(24659..25384)
                     /locus_tag="VN178_04110"
     CDS             complement(24659..25384)
                     /locus_tag="VN178_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HWS80186.1"
                     /translation="MTPEPLGRSLSDFTVELDVYSGPYEWLLALILKDELEIFEVPLR
                     ELVASYLLSRRPGAANGLERDTDFAGSAAALVLLKSRTLTPAVDADPEGVEDETLSPE
                     ELAERLTEYLKIRRAAEHLKDRFASNAGHYASGHTMPPRPGSLHVDPNRLSLAARRAF
                     SRILEPPVSHLGPITVTLQQLAGIIRTALAKGTISYESLTSDMDRLNKAVTFAAALSL
                     AQEGSLRLVQTEPLGPLTLEPSR"
     gene            complement(25381..26514)
                     /gene="ald"
                     /locus_tag="VN178_04115"
     CDS             complement(25381..26514)
                     /gene="ald"
                     /locus_tag="VN178_04115"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWS80187.1"
                     /translation="MRIGVPTERTQGESRVALAPHAVYELTRAGHRVFVEAGAGEASS
                     IGDESYEEAGARLVKDAAKIYADSEIVVKVYGPVEGEYEYLREDLILISFLSLPVNLE
                     LMRALIDSRCIAFAMETIRDARGRLPVLTPMSEIAGRLTPQIGAHYLQRPFGGRGILL
                     GGSTGVRPGRVVILGAGIVGAGAASVASGLGAEVVIIDRDLDRLRQIEDTRLPNTTTL
                     AASSLGIRDAALRSDLLIGAVHVVGTRTPALVDRETVSEMKERSVIVDVDVDYGGCVA
                     TSRPTTLADPTFVEYGVTHYCVKNVPASVPVTATRALSNALLPYLRKLAGVGLSDALG
                     ADEGLARGVAVAEGRVVDPVLARDTGVEYNQLSSVLPLHSEAR"
     gene            complement(26622..27125)
                     /locus_tag="VN178_04120"
     CDS             complement(26622..27125)
                     /locus_tag="VN178_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80188.1"
                     /translation="MFPTGDPAGTVATRTAGLLEFSATLSWNGLEDLPKAPALAAPPG
                     TARCSVFESVDPETGKREILGEMLALSERDLGSALENVLRTLATSAALSDAVLEIRTT
                     RVTPPASAPATALQALALDLWAAGFGVVFAPSWTPVPEGAVGLGILGAEQELRSFIEA
                     HPAWKAP"
     gene            complement(27179..28834)
                     /locus_tag="VN178_04125"
     CDS             complement(27179..28834)
                     /locus_tag="VN178_04125"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWS80189.1"
                     /translation="MSETKYVFVTGGVVSSIGKGTSAAALGMLLKSRGYRVVLQKFDP
                     YINVDPGTMNPYQHGEVFVTEDGAETDLDLGHYERFLDENLGRLSNVTTGSVYWEVIT
                     RERRGDYLGATVQVIPHITNEIKNRIGRLGQDKDIVITEIGGTVGDIESLPFLEAIRQ
                     FRNDVGRKNVLYVHVSYVPYIEAAGELKTKPTQHSAQRLREIGISPDVLICRADRPIS
                     DDIRRKIALFADTELDSVIAAEDAPNLYAIPLNLHEEGLDDLVLDKLGLSAPHADLGE
                     WRHLVSRMLEASQTVRVAIIGKYIKLQDAYLSVVEALAHAGGAHGLKVELDWVDSEQL
                     LDGPSTEERLSGVDGILVLPGFGHRGAEGKIEAARYARESGTPYLGLCLGMQIEVIEF
                     ARNVAGLEMANSTEFEQDTPHPVIDIMPDQVGVDMGGTMRLGRWPCQIIPGTLAEEVY
                     GSGLVHERHRHRYEVNNAYRDTLADAGMVFSGTSPDGHLVEIAEIGDHPFYIGSQFHP
                     EFRSRPLRPHPLFYGFVGACGETLKETAGLKTEPLRPEQGATR"
     gene            29268..29771
                     /locus_tag="VN178_04130"
     CDS             29268..29771
                     /locus_tag="VN178_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80190.1"
                     /translation="MKSARALLAVTKDDMANLQCALPAQARAPGLRVVLRLFDPDLAA
                     RVERTTGIYLSRSVSALAAPAFVAAIFGRKPEAVLPIGTEVLQIVDLKVEKASDVGTL
                     EDRCQARVLAVGATAFPVPELTVSPGDEMRVVGTNRGLMELARQVAPSALTAWSDVNE
                     HRGETAP"
     gene            complement(29833..>31053)
                     /locus_tag="VN178_04135"
     CDS             complement(29833..>31053)
                     /locus_tag="VN178_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWS80191.1"
                     /translation="LGRLLRAQNLAGYEVVGLSGTSGGAVCALLAWHKLLRGDAAGAV
                     EDLDAFWRDNSATTPLEQVLNNWTVWAGKVQNFVGIPAISPYDNYFSDLGLEEFRRLL
                     ERRIDFRNVRVQPEGSYPVLLVGAVDVLSGEFRTFNSRRDAISAKTILASAAIPTLFR
                     SVCLDDGGTYWDGLFSQNPPVRELTDERPDEIWVIQINPKERESEPRTIAEIADRRNE
                     LSGNLSLHQELGFIERIDQMLEEGHLVSDGKYKQIVVRVIELSRSRFSRFLGTASKLN
                     RDPHFIRDLMSHGEARAEEFLAALAFEDAWKSRDTAAIVGFFADNAQIFSSAPFANDG
                     IHKGTREVRTFVADILARGVRVDPTRKQVARNGVAWTVRMPADAGSPGRLKGVVEAEF
                     QGGSIIALRLGGET"
BASE COUNT         5330 a         9860 c        10029 g         5834 t
ORIGIN      
        1 atcgaggtca acgaggagac actggcgcaa tccctggcca tcctggacgg ggaggggcgc
       61 gtcatcgggg ccgccttcaa cgagacgatg gcgcccttgg acgtagaacc cccgttgcgg
      121 aaggacgatc cgttcctcga cgccgtgata tcggtctggg cgccggttta cggtgcattg
      181 ggcgcccagg atgccacggc cctggcggcc ctctctgaac ggtacccggc attccgcgag
      241 gcgtacgaga agggcaaggt cagccaccac atcttgatcg cccgctccga cgaacttccg
      301 aaagaggata ccttcgagct cgtggcggcg ggtggggagc gctgccgcac gctcggctac
      361 gaatacgtgg tcaccgaggc gaccaaccag tggacggggg cggccttcga ggccctgggc
      421 ggggtgcggg tgcacttcgc cccgttcctg gtgcagcaga ccgttcccaa gagcgacgag
      481 ccgctcgagg acgtcctcac gagcccgaac gggttcctcg ccgagaatga cagcgggggc
      541 atgttctacg tgatccgtct cgcctagagg aactgtgaac caattgggct ggaagcaccc
      601 gccccacctg aaacaagaga agcgacgaac acaaatcggg cagtagcgcc tcagttgtgc
      661 agatggcatc atggcggcgc atgagaaacg acccaccgtg gaggtgggcg tgggaaaggg
      721 aaatacgatg cacgagacta acgacgtgga gttcgccacg acgagcgtcg cgacagacgt
      781 cacgctccac tacgccgaac ggggcgaccg ggaagggaaa gctattatct tcctgcacgg
      841 ttactccgat tcgtggtact ccttcagccg ggtactgccc ttgctatcgc cgtcttacca
      901 cgccttcgcg cttactcagc gcgggcacgg agactcggat aagcccgaat gctgttatac
      961 gccacatgac ttcgccaccg acgtggatgc gttcatggat gcgctcggca tcgatatcgc
     1021 gaccatcgtc ggtgcttcga cgggagccgt ttttgcccaa cgagtggcgt tgagctaccc
     1081 acgccgcgtt tgccgtctcg ttctgatcgc tccgcaaacg ccggcgcatg agaatgaggc
     1141 gctttcaggg ttacgggagg aggtgcgtac cctggaagat cccgtgccgc ccgagttcgt
     1201 tagggagttc caggagggca ccatccacca tccggtcccg caagagttcc tcgaaacggc
     1261 gatctccgag agcctcaaac tcccggccca cgtgtggcgc gactacctgg accaggcggt
     1321 actttcgatc gatcgggatt atgtagtcga gcttggtgag attgatgtgc cgactttgat
     1381 cttgtgggga gagcgggatc ccctcttccc gcgcgaagaa caagagcggc tcgcggcggc
     1441 gatccccggt gccaccttga aggtctctac cctgaaagtg gccacgcgac gcactgggaa
     1501 cgaccggagt gggttgtacg ggatcttgaa gcgtttatga aagagacacg tcccgcccgg
     1561 tagtcctccg ccacagatcg aacagtgggt aatggtatat atggaccgcc cgcaggaaag
     1621 gagaacgttc atggagacca gagcacaccc gcccggcttg caagacgtcc tggagtttcc
     1681 gttggtcgaa gcgctgttcg ggaggcgagc ccgccggttc tctttgggcg cctcgctccc
     1741 cgacgggccg cttgagttca cctcccggca cgatcctttg ccgctctccg agctggagca
     1801 gatgctggtc ttgacggccg cggctggcaa caccggatgg cactacatga tcatgcgcca
     1861 cgcccgctac gctccccatc tctcgaacta ctcaggagcc gccggcggcc gaacattccc
     1921 atcggcagcg ggatttcaca ccagcgagtt gttcttcacc gacgacgagg gcgtctacct
     1981 attcgagacc cgcgacgccc ccgctctggt cgagcgtacg accgacggac ctcccgacct
     2041 cgacgccatc ctggacgccc accggggccg gatacgcaag ctatccgacg gtcgactgta
     2101 catccccgcc gaagagccct acatggaggg gcataatacc tggtgcgcca accgtcctgg
     2161 gagcacgctg ctcatccccg tcggggacat agcccagcac atgatcgccg tcctctgctt
     2221 tatggtccag aatggctacg ggatgtacga tgacgtcaac ggggagcgga tcccaggtct
     2281 ggagcgcttc tcccacctcg tggacttgga ggagcccctg ccactgacct ttatggagac
     2341 ctactcgatc accgagtgca ccgccgagct ctcgacctcc tgttatgccg gcatgctgat
     2401 gctgcaggcc atgggtctgg gaggctggat gttcgacggc atcgaccgct acacggtcct
     2461 cggggcgagc ggcgatccgg atgtgcctgg attgggcttt cgctacgaca ccgatgagcg
     2521 ctggccacta ccgaacccca cgggtttacc aggagtcttc gcgggctatt gcccgcccca
     2581 ctaccccgac atgcgggccg ccgtcgaagc cttcgccgag cggaagttcg gtccgggagg
     2641 accgttcaac ccggacacac ctggtccctg gaaggagagc accagggtgc gcagcagcgc
     2701 acaggtccac agcgaggagt tcaaggagtg cgtcacggtc atggcgcagt acatctacga
     2761 ccgcttcggg aagttccccg gcacggtccc gagcatcttc atcctgactt acttgcaggc
     2821 ccaccacctc gagctggagt tctatgatca ccacttcggc ccgggggcgt acctgcgaac
     2881 ccacgccgac cacatggccc gctggcatgg tagaggtgag gaagaggttt gaattaccac
     2941 atccgataga tctaatcgct gtctggatcg gtccgggatc gccgagatct cgcccaaagg
     3001 cgtacctcgc gcacccactc cgggcgcccg gtcctcagac cctcctcgag gaagcgcgcg
     3061 aggtcttcgc tcgtgagggg cggcagggtc agcggttagg cgatcatcag cggttcggca
     3121 aggctgggac ggctagggga cgtcgaacga acgaagggtt tcttcgaggc cggcgcgaat
     3181 atcggcttgc gcgtgccagt cgaaggtacg tccggcgagg gtggagtcta cgcagctacg
     3241 gagttgctcg cctggcttgg acggtccatg ccggggtgga aggtctttgg cggagatctc
     3301 gagtagcagg tcgtagaggc ggttcacgct ggtctcggcg cccgtgccga tgttgtaggc
     3361 accggagggc acgtcgcttt cgagggccag tacgtttgcg cgggcaacgt ctttgacgta
     3421 gacgtagtcg cgggtctgct caccggtgcc gtttatcgtc gaggtggtgc ccctggccag
     3481 gttgccgcag aagatcgcca cgacgcccgc ttctccgtgt gggtcctgcc tgggtccgta
     3541 gacgttggcg tagcggagcg ctacgtaggg cagggcgaac tggacttggt agtagtgcag
     3601 gtagcgctcg cccgcgagtt tggagacccc gtagggggcg acggggtact gcgggtgatc
     3661 ctcgggggcg ggaaactggt gctgctcgcc gtatacggcc ccgcccgtgg aggcgaagat
     3721 gaccttgtgc acgccgtatt cggtgcagtt ctcgaggagg cggaccgttc cgaggacgtt
     3781 gacctcggcg tcgaagtccg gctcccttac ggaccgcctc acgtccatct gggcggcctg
     3841 gtggcagagg gcatcgggcc cgaagtctct gaagatctcg gtgcacccgg agcggacatc
     3901 ggcctcgtag atccgggccc ctcttggtac gttctcgcgc ctgcccgtcg agaggtcgtc
     3961 gacgacggcg acttcgtgcc cactttggag caggtgttct gctatgtaag atccgataaa
     4021 cccggctccc ccggtcagga ggacccgcac ctcaggagcc ttcgccgcgg gtcgtggtcg
     4081 acgcccggga tctggtcttc gcctgagagg cgaaccaatc cagggtctta gcgaggccct
     4141 cgcgggcccc ggtccgcggt tcccaaccca gcacttctct ggcgcgggtt atgtcgggac
     4201 aacgttgttt gggatcgtcc tcggggaggg gctgatggga gatatcgctc ccggatccgg
     4261 agagctctac gatcatctcg gccacttcgc gcacggagta ctccacaggg ttgccgatgt
     4321 tgacggggcg tcgctccccg ctcttcatca ggcggatcac accttcgatc aggtcgtcga
     4381 cgtactgcac gctcctggtc tgggtaccat ccccgtagac ggtcaggggt tcgccgagga
     4441 gggcctgtcg tatgaagttc ggcaccatcc tgccgtcgtc ttccctcatt ttggggccgt
     4501 gggtgttgaa aatacgcacg atcctggtgt ccaggcggtg gtggcggtgg taggccatcg
     4561 tcagggcctc ggcgtaacgc ttgacctcgt cgtagacgct cctcgggccg gtggtgctca
     4621 cgttgcccct gtagtcctcg tgctgtgggt gcacgagcgg gtcgccgtag acctctgagg
     4681 tggaggcgag cataaagcgg gcgcccctgg cgcgggccag ccccaggcag ttgtgggtgc
     4741 cctgggcgcc ggtcttgagt atctcgatgg ggatgcgccc gaagtcggcc gggctcgccg
     4801 cagaggcgaa atggtagacc tcctcgagcc ctccggcaac gtccatgtgg gtgtttacgt
     4861 cgtgctcgac gtactcgaag tccgcctcgt tggccaggaa aggggccacg tttgcaaggc
     4921 tgccggtact caggttgtcc acgcagacga ccttgtaacc ctctgcgagg agtctctcgc
     4981 agaggtgact gccgatgaat ccggcccctc ccgtaaccag cgccctcggc cttctggcgg
     5041 cgggcctaga cgttgtcatg gtgctccaag cgacccgttt ctccccacac tcccggggaa
     5101 gtaagcctac ggttcataaa ggggatgtta caggaagggt gagtacgcgg ccagcctcga
     5161 ggacgggccc tactctttcg gcctggtgcg tcttaccacg gccttcacac ccggtatgcg
     5221 aaggacgttt ttcgccagcg catcagcgag cttgtagcgg ccggcgctgc gatagtcgct
     5281 gagtttcgag atgaccagca acctttcctc tttgagcctc gagttgcgct cttccagctg
     5341 ggaggctcgc ttctcgagct gcaagatgcg cttctcgagt cgagaggtct gactctgaag
     5401 ccccttgttc tcgattttca ggccggcgtt tcgctcttcc aaccgggatt ttcgcctttc
     5461 gagctgggag atgcgtttct ggagttctgt gttgcggccg gccagcgatt ggtgttcgcc
     5521 tagcaggtcc tggagttgcc cgctcagggt gtccgcctcg tccctgtagg cgctcacgat
     5581 gccgatcaac tcttgctccc gacccgccag ccacagcggg tccaaccgtt cgctgaacag
     5641 gtgggcgagc tccgtgaggg acctctgtac ctccgaagat tcctcgtcgg gatagacgat
     5701 cccgagcttg ctcgtcgcca tacccgaact gaggtctccg aagagccgaa agtgatactt
     5761 tcccggcgct ctctgcatac agtctgcgat tcccgcctgt acgaacggtc cctcctcacc
     5821 cctgcagtgg tccaggatgg cgaaggcgcg cgggttggca tcgaagatgg cctgaagatc
     5881 cgcgtccacc gcctccctgg tgcgcgcccc gcccacgtac gccagcagcg gttcgttttc
     5941 ggggagttcg tgagccagct tagcggaggc gccgcttagg tcgcctgggc gaagctttat
     6001 cttgacgctt tcgtcggcga actcggcgag ggccgcccgt gcgatgtcaa tactcgaggt
     6061 ctcggcggtg tcctcggtcg ccgacgggtc tacgccaagc accctcgaca cactgggttg
     6121 gcccgcgaag tggaaggtgg ctatccccaa ggacgtgcct acctcgagca cactcagctc
     6181 gtgcggataa caccgcaaaa agagcgccag gaccagggca tcgacgtctt tgatggacgc
     6241 cgcgtcgagg aggtctttag gtagcgcggg gaagagcctg cttatggttt tcttgtattc
     6301 ctcgagccgc cgcgtgtgct cgacgatctg tgaggtccac tggccgacgg ccttagtcat
     6361 ggtgcctccc ggtagagccc accgtcagtc aatgtagcct accgccggat ggtagatgcg
     6421 tcccactctc acggcatccc actctcgcgt gccggtctgg gcgaacatac gccgcagcaa
     6481 ggtaaccacc tcgggcgcgg cagagcgagg gggaggcccc ttgtcctccg ccgtctccgc
     6541 gtacatagat ccgggagtca gatggtaata gccaagcgcg acgggtacga aaacgagctc
     6601 ctcttcctcg gcgatcaggt gtaggatcat ctcccaatct tcgtaaccgt agagcctctg
     6661 gtcggttacg aagccccctg ctcctagtac tttcatcgtg tctagcaagg cgaaggcgtc
     6721 gatgtagttg ccggccgtga ggcgcatgct tgcgacgttg tccgacctta tacccgtcat
     6781 cttgccctct cgcttgtcta tcaacagacc gtgcaccaac gcggcgcccg tcctcacgct
     6841 gctctgcaag aacagcggca agttgtccgc gactagctcg ttgtcggcgt ccagcatgca
     6901 gacgtgcctg tagcgcgaga tccgcatcgc caggttgcgc agccttggca gaccgaggtt
     6961 acgctccagg aagaccgttc taagcggcgc gtccccgtag aggacctgaa ccgtgcgcaa
     7021 gaacttctgg gaaccgtcgc gggaggcatc gtcgataacc aggagctctc ctgagtgacc
     7081 ggcctcctcg aggcgttcga tcgcccgcag ggcgctgcgg atggagcggg gaagatatgg
     7141 cctgtggttc cagttcggga tgaagatggt tacgccgttc tcttccttgc tctgcccgta
     7201 gagcgactcc gaaccagggt cggcgaggac ggtggactcg atacgattcg tgctctgcag
     7261 tatcttggct tcagacaaga cgtaacgccc cggacaaatg ctcccccaac cgtcccctaa
     7321 agacgtagaa atcgctggca cgacgataca tctcactata ccccatgtcc cgcacttccg
     7381 tatcgaatgc cgacaccgaa gcgtagacgc cgggaacgtc gaaccccacg gcggcgaggg
     7441 cgggatctat cttgcatggt accgtaccgg ccaacagcgc gacgatgaaa cggaaactct
     7501 cataatacat gccctcacgt tctgcacccc acagatagta tctggccttc gacaggatcg
     7561 ccgagagttg cgggccaccg agcgtcggct ctggcggggt cgtgttcttc tgcctggcat
     7621 gcaagaggca gaagcctcct gggtcgacct gatgctcgaa gagcgccccc aagagatccc
     7681 ggttcgtatc gcttctgggt ccgaccagga cccaagggat cgtacgctct gcccggtttg
     7741 cgggctcctc ggccaacggt tcttcttctc gggtaaggcc gttgaacaca aagtggtaag
     7801 ccaggttggt caccttatag tggctgtctc tctgggagac gaagccgagg tcgaagaggg
     7861 cgtcaaagtg gatacccctc ctcagccgtg agaggtatcc ctggctatgc accggaccta
     7921 ccgacgccag gatgcgcttt cgtgcgcggg ccaccttcga gaggaaggcc ccttcgttgc
     7981 cgctggtctc gatgcactgc cccggactca ctacgaccag ggtggtgtta ttcagatcat
     8041 ctggggccag ggcgctgacc tcgggcgagg agtacaccgc gacccggtga gaggcgtctc
     8101 taaagccctt ggacagtcgc gaagccaggt tctggtcgcc cgtcgtgggc gggcgtacga
     8161 ttgcgagtgt ttcggtctgc atgctgcgtc tacgatatca ggcctcatcg ctcaattcta
     8221 taagtgcccc gcagacatcc ctcgcacgga cggcgcccga ccgtgcgaga tcgttctcgg
     8281 ccgggtcgat ccgcctcgaa gcctacgacg gcgtgaatcc gtacaatagt aggctcccag
     8341 agcgagtcag ggagagttgt gatgaacgag aacacgggtg cggcgaggga catgggtctc
     8401 acgcgaccct actccggcgc tcgctcccag ggaggcaagg ttagcgtggt gatcccctgt
     8461 tacaaccagg cgcggttcct cggggaggcc attcggagcg tgctctgcca gggctacgcc
     8521 gacctggaga tcgtcgtcgt cgatgacggc tccagcgacg gcgcggcgga ggttgcatcg
     8581 ggctacgcca gggtggatgc aaggatcagg ctcataaggc aggagaaccg gggccttgct
     8641 gccgccagga accggggcct ggcgggggtg gggggggatt atgtggtgtt tttggactcc
     8701 gatgaccgtc tggtttctgg agccctcgag gtcggggtga gggagctaga ggagcatccg
     8761 ggatgcgcct tcgtctccgg actgtgccgg aagatcgccg ccgatggttc ggtggtgcca
     8821 gggtgggagc agttccgggt cagggatgac ccttacctcg agttgctgcg cagttgccca
     8881 atctacgtac cggcggtgat gtatcggcgt tgtgttttcg acgccgtggg cgacttcgat
     8941 acctcttaca aggcagccga agactacgac ctctactacc gtattctgga gcgtttcccg
     9001 gtctactgtc acgacacgat cgtcgccgag atacggcgcc acgacgcgaa catgacccgc
     9061 gatcgcacct tgatgttgaa gtacaacatg gccgcgctgc gctcacagcg caaacgcgtc
     9121 aagggggacg cccgctacaa agcggcctac aaagccgggg agaggctctg gcgagactgg
     9181 cacggcgccc ccgtcgtaaa ccaggcaaga tcccacctca gggagggacg ctggcgagag
     9241 ggtctccagg gaatgctcgt gttgttacgc tactacccgc gggggatcct cctcgtggcc
     9301 aactggggcg tgcaacgtga gagactcgcc cggcgcctgc gccatcagca gcgggagcta
     9361 gagacgcaca gatacaagct acagcaactc aaggtcccgg gcgaaacacc agcgcaaaac
     9421 ggcgaccgac gggacaaaga gctccggatc gccatcaaag aggagcgcca aaagatccgg
     9481 caccacaagc agcgcataag gcgcctgacg tcgcgcatgc agcggcgcaa acaacgcacg
     9541 gatgcgctcc gcaaaggctt gcaatccgtc aaagcccgcc tccgcttgac atcccgcgga
     9601 taggcgtatc taaacacata taataccctc tgtagaacga acacacccca cggtcccgcc
     9661 catccactcc accatggggc atggacagca cagacccggt tcgggtagtc ggggtcgaag
     9721 gtccatgcca aggcgggcac tggtcccggt gggttcggcg ataacgcaag cgacgtcctt
     9781 ggttcgctcg aactagacaa ccccgtgtgg gtcgacgacc tcccgcgggc acgggctctc
     9841 tctggggccg cgcaccaact tccgagaatt cccgttatgc gaagttcgcg cttttggatc
     9901 tgcggtgaga agcagcacat cgtgggaaca catgcctgac gcctgcttaa gagtgagtta
     9961 gggctaagct tggacggctt gcaactttgg agcggcgcga gtacagttcg gactctgtcc
    10021 cgcagcaagt gaatacaagg ccctctccgc attactctta tgaacccgcc aaaatactca
    10081 gatctaatct gcgcgctgcg cttgtacccc agcacacctt tagctgctgg gctctcgaaa
    10141 gaacgcactc tgccaacgcg gttaagaacg atgataacgt attgctctta tacatacggg
    10201 cacatgggtg taggatgccg gtgatgttgc ggtcccgatc atcccatctg gatcgttcgg
    10261 ctttgccgcc ggcagcttta tgacttcgcc gaacagcacc agagcgaaat tcttaactct
    10321 ctggtcgggt agtctatctc gggattagag agttcttcag atggcccgag accgaagctc
    10381 tggccaccta cggagttggc gtgggatata gtattatgtg actttatagt cggcatagct
    10441 cgccaagcgg atgcccagtt ccatcggcgg tttgccggat gcgaggatcc gtgagggtgc
    10501 gcacttccgc ctcccgctct gcgtcgtaaa cggccgcgaa gtccggcgag gggtaacccg
    10561 gatggcacga aaactcggtc catttctcac ccacctcctc gcgaagcatc cgttggagaa
    10621 aaggcacgct cacgtacttc aggttcgtca ccatccactg ccactgggcg tagaagccac
    10681 cgacgaaacg cacctggcca tcatcgcgca ggggcacacc gagtggctcc accagctccc
    10741 gaaacagtgg catcagcctc ccccccggtg cgcgtgacgg tgggagtcga cgtgggtggg
    10801 cattcttccg agcaagcgat agaactcgtc cagctgctca tgaaaattga cgtggaccgc
    10861 ttggaggttg tcaatatcga actcccgttc atcttctccc cagacatccc agtgcagtcc
    10921 gacggccagc tctggatggt cgaggctcat ggacacggcc tctcccacgg cgcggccagt
    10981 gaccatgagg ctggcactgg taacgatgcc gcgggtgtgg cattctagga tgcctcggtt
    11041 cacgccggtg gacgcgccaa agtcgtccgc gttgacaatg aggcatttct ccaccttcta
    11101 cctcctcgct tactatcttc tcgccgacgg acatctaaca cccttagagc ctcgcgaagc
    11161 gtgattagtc accctcgaca gaggctacgg ctatgcgaga ccaatgtttg agagcaggcg
    11221 cgcggcgacc gagcgggcct cgaagtattc ctccgctatc gtacgcgtcg cttcgcagtg
    11281 ccgtcggtag tcgccgttga ttgcttcgat agcacccagc gcctcaccca tcgtggtgaa
    11341 ggcgaaaagt ccctcacccg taggcaagac gttaccgaag cccgtgtctt gagcgaccac
    11401 gggcttgccg catgccagat agcaggcacc ccgttcgctg aaccacccgc tccggaggcg
    11461 aacgttctga tccttggcaa ccgtaaactc ggcgcgggag cggcggaagt aggcagggta
    11521 atcgccgaat acgtccagcg acatctgcaa gggagacact agcgcccagc cgtgagcctc
    11581 caggcgctca cgactagccc aatcgtccac gttcatcgcc agctcaaacg gttgtttagt
    11641 gcatcgtggg aggcccagga atttctccca ctcgtggtgt ttgctccacc tatacacctc
    11701 accgttgtac tccacgtcgc tgcccgcctg acagtagttg ccgatagtgg tgaaggaacg
    11761 aacagagcca gcgaagttcg agtgagtcga gcaagatcgc accgaaggct atgtctccga
    11821 tccagagcaa ccacgggagc caagctcccg gtcggacgct gatcctacgg gctcctccgc
    11881 tgtgatgtct tacccctttt gtcacgaccc cactctacag accgatcgta tcccacgctt
    11941 tgcgaagccc agctgtagac gcacttctaa gaaccctcga ggcttcgacg catctgccct
    12001 ccgcgcgctc gaatttgtcg gcgcccccta caggtacgcg gtctgaccgt cctcctgtgg
    12061 gggtcccgcc tcggtctaca gtgaattcgg tcggtcttaa cactttctgg ctattggtag
    12121 gttcgcatgt acggatatac atgaccccca tatctgtcag agcaaggaaa aggccatggc
    12181 ggtgtaccct ttactacgtt ctccagtcag acgttagcgg agggtcggct agcagcgtag
    12241 gctgcgccaa tcaatcgtgg ctttgtatta tgcgttcctc tgaggaaagg tggccgggta
    12301 caatgcttct tgaaggcgac tctagttgag gatggacgag gcgtgatgcg agtggagcag
    12361 cttcgtggca tcgaggacgt agacgcggag acgtggcgca cgctggagcc gcctgagttc
    12421 cctttcttcg acttcgagtt cttgcgggcg ctcgagcgct cgggtagcat cggccgccgc
    12481 agcggatgga ttccggtcta tttggtgtgc aaggaagggg cgcgtgtcct cggcgccctc
    12541 tgtctgtatc tgaagacgga ctcctacggg gagtacattt tcgactggga gtgggcgcgg
    12601 gcataccagc agtacgggct ctcttactac cccaagctgg tggccgccgt gcctttcacg
    12661 cccgcgacgg ggcccaagct tctattgcgc cgcgacatcg atgtcgcgac ccgtagcagg
    12721 gtggcgaggg ccttgctcga ggcggcggag gatctgggga cggagtacgg ggtaagttcc
    12781 tcgcacgcgc tctatctacc cgagcatgag ctcggggagt tcaagagaag aggcttcgcg
    12841 gtgagacact cgctgcagtt tcactggcgc aaccggggct acgagacgtt ttcggactac
    12901 ctgggggcgc tctcggggaa acggcggcgt cagatcttgc gcgagcgacg ccaactggag
    12961 gaactggaga tctctcacct tacgggagac gcgctcctcc ccgaacacgc cgcatcgatg
    13021 tacgagttct acctggctac gctggacggc aagtggggag tgccttacct caacagggcc
    13081 ttcttcgacg aggtgttcag gaccatgaga gaccggacac tgctgatcct ggcccgcggc
    13141 agggccgacc gtcccgtggc gggcgcgctg ttcttcctca aggacagcgc gctcttcggc
    13201 cgctattggg gagctgcgga gcataggcgc aacctgcatt tcgaactgtg ctactaccag
    13261 gctatcgact tcgccatcga gcggggcttc gaactactcg aggccggcgc ccagggagag
    13321 cacaagctgg cgcggggctt tctcccttct ctcacctaca gcgcccataa gatcagggac
    13381 ccggccttcg gacgcgctat cggagagtac atagaatcag agaagcagat gctccaaaac
    13441 gtcatggagg agtacgcctc ccacgatccc tacagacgct gatccgtcct tctacagcgg
    13501 caaggtatta tactgataag cgcgggtggc gagctcttct gcgcggcgga tgtatcgcat
    13561 ggtcgtcttc gggtccgagt ggccgaggaa ctgttggatg acgagcaggg gcgcctcgtt
    13621 tacggccatg ttggtgccca cggtgtggcg gatggagtgg ggtgagatgg ccttcgttat
    13681 gcctgccgcg cgcacgtact tcttcactat gttctggacc gagcgggtgg taaggggttt
    13741 gtctttgccg accctggagg tgaacagtgg tttgcgggaa tcggggtgag aggtgaagct
    13801 cctctcggtg agcaggacga agcgttgtac caggcgccag aggtccggtg agatagggac
    13861 gtttcggact ttgtcgccct tgccaaggac gcgcagcagg acctgatccg ccaccgcctc
    13921 gcggaagtcc ccgatcctga gtccgacgat ctcggcctcc ctcaggccgc aacccgccat
    13981 cacggcgagc atgacgtagt cgcggtagtt ggaggccctg gctgcgttca acatgcgcga
    14041 gagctcgtcc tcggtaagca cgttgtaggc cgggtcctgg cgcacgcgcg ggctcttggc
    14101 aaaaaaccgc agagcctcgg ggttcaccgt cgtggcgcca gccatgtacg tgaagatcag
    14161 aaaccgcctg agcaccgcaa gcttcttggc cgccgtcgcc gtcgcgtagg tttctagcag
    14221 atgctccctg tagagcgaga tgtcctccgc cgtcaactcc ccaagcccct tccccacctc
    14281 gcactccaca aaggagatga acagggaaag ttctgtatta taagttttta tggtctgggg
    14341 gctggtaagg gtacggaggt agagggcgac gaggcggtcg gcttcgggga tgagctgatg
    14401 gagggatggg gttgtttcgt cgagcggttg ggggaggtta gtcttgtggg tcatggtagg
    14461 tgatcaggga gacgacgggg tagggctcga gcttttcgcg gccgcccagg taggcgagtt
    14521 ctatgaggaa ggccacgccg gcgaccacgc cgccggcgtt ttcgacgagc tggcacatgg
    14581 cgcgggccgt acctcccgtt gcgaggaggt catccgcgac gagcacgcgg gtaccttcgg
    14641 ggatcgagtc ggcgtgggct tccagggcgt cggtaccgta ttcgaggtcg taggtggcgg
    14701 agatgatctc tctggggagc tttttcggtt tgcgcgccag aatgagaccc tttttggcgc
    14761 ggtaggccag ggcagtgccg aacacgaagc cgcgggcttc ggccgagagt atgcggtcgt
    14821 aagcaatgcc ttcggtggat tcggcgaggg cgtccacggc ggtgtggagg gcgtggccgt
    14881 cttctatgag gggggttatg tccttgaaag agacgccctg ccgcgggaaa tcggggacgg
    14941 tgtggatgtg tttgcgtaga gcccttgcgg cgtcttgagt cccctgcgcg gggccgctca
    15001 cggggagaac gtgtccagca cgttcgccat catcagctcc ttgatgcgcg agtcgacggc
    15061 ggcgaggtgg cgggccttgt tcatggcctc gcccacgcgg ccgtcatcac cgctgcgcag
    15121 gtcagggttg agcacctgct tgaagagggc ggcctcgcgg tcgacggcgg ccataatgcc
    15181 gcgcaggtgg cggggctgga tggagtactt cgccatctcc agggccatgc gcgcgatctc
    15241 ggcgtcgcgc tgcgtgagct cgccgctgcg cccgatcacg cccacgctga tgagctcttc
    15301 gacgaagcgc atctccgtct ctaaggcatc cgccagctcc tcgcgcgtgt acgtcctctc
    15361 ttcgaggacc ctcgagaggt cccccgccga gtcggcggcg gaaaaaccgc ccccgccttc
    15421 aatgcgcttc tttatgacct tgagcggcag atactccttg tcttgcaacg taaggatgta
    15481 gcggaggagc tcgatatcct gggacgagaa gagcctgtag ccgccgcgcg tgcggctgag
    15541 gttgatcaga cgcctgcgct ccaggtagcg gatcttgctg accgagaggg ccgggaactc
    15601 gggtctcaga cgctcgacaa cctcgccgat ggtgaagctg tctttggtgg cgctgtacct
    15661 ctcctgcgcg taaccttcgg tggtgtagcc cccgttgatc tccctatcca taccgtcgtt
    15721 ttccacgctt ttcactccca tcctctcgac tacagtgcac catgctctag gagaacacga
    15781 acctgaaacg gtacttgcct acctgtatct cgtctccgtt gcgcagatcg acggcgtcga
    15841 ccctgacgcg gttgacgtac gtcccgttca ggcttcccac atcgcgaatc ctgtacccgc
    15901 catctccgtg cacgatctcc gcgtgtttgc gcgagacggt gacgtcgtcg aggaagatgc
    15961 tgctctcctg tccgcggccg acgctgacga cgtcatcgcc gaggtcgtgc atcctgcgtc
    16021 cggccgtgcc ctctacctga tccatctcta tcaatgcggc cccttcggcg ccgcccgatg
    16081 tgccgggctc gacgtcctcg ccgaaactcg gcgcgtagga gacggtgctc tggccaaggt
    16141 cgagcctggt gccgcattcg acacagaact tcatccccgc cgagacctcg gccccacacc
    16201 caggacactc tttcacctgg tctcctcaac gggcgaatcc ccgagcaaca cccgcgccag
    16261 gcgcccggga gggagcgcag cggcgcccct ctgcacgagc tcggcacgga tcagatccat
    16321 cctcccccgc aggatcgcac gcctgtaagc gagacgtcgc tcctcctccg agagcgccgc
    16381 aagagtgtcc ctgagatctt cctcagagag ggccgtgacg tcctcggcgt tctcccagtc
    16441 gaatcccccg tgcaaccccg aatcgtccat agcgcaagta taacctaccc tgaacctgaa
    16501 gtgcaatctc agcttgagag caaggcctgg tcgtcgatca aggacttgac caggtcgacg
    16561 tactctccca taagccgtgc cgggctcccg tcgccctccc cggactcggc gtagacgtag
    16621 aacatggggc tgtcgggatc ggggagcata agcacccagc cgtcttcgac gttgagcagc
    16681 acgccctcgg cgaggatggc gtcggggtcg ccgccgaact tctcggcgag gccgcgcatc
    16741 acgcgtccct ttgcggacca cgggcactcg atgtgctctc tctggacgtg gtcgaaggag
    16801 tctccgaact tccggcgcac gccggagagc ggcgtctcgc ggaatacttc gagggcgctg
    16861 gccagcgtca taaagcagtc cggtgacggc aggaaggccg ggaagatgta gcggccgtcg
    16921 gcgagtgagg cgaggtcggc cccgatgtct gcggccttta tggcgacgtt ggagaggcca
    16981 gtgcggctca tctcgaccgc tccgccgccc tcctttacga gtctctcgta ggcgcggctg
    17041 aggttgatgg ggagcacggc gctcttcggg cgcatctggt ctatgaggca ggcgagcatg
    17101 acgtcgtgcg gcacccactc tccctcgtcg tcgacgaact gcacgtactc ggcttcgggg
    17161 ccgaccacgg cgccgaaggc ggcgtccacc gtgggcacga tgcgggcgac gcgctcgatg
    17221 ctctcctcca ggttcaaccc ccccgcctcg ccgaccgcgt tggcgttggc gaagccttcc
    17281 atgacgacgg cgctcacgcc gagcctggag aagacccggc tcgccacgag gccggcgacg
    17341 cccccgaaga agtccacgac gatggtggcg ccggaggtcc tgttgcggtc cacgacgcgc
    17401 tccaggcgct ccacgaactg ctcgagaacg cggcccgggt agatcaactc ccctatgtcc
    17461 ccgccgtagg ccctgcgata ctcctcgcgc acgaaggcct tctccacgga cctctcatcc
    17521 ccctccgtta tgggggtggc gtcggcggag aagaacagga tctcgacgtc atcggggtcg
    17581 ctgccggcgc ggacgtgcgc cccggccgag gacttgccgg cgagcacgtc gtggcggacg
    17641 accccggcgt gggcggccct gaggtccctt atgttgatcc cggtgccgag cagcgccgcc
    17701 gtcatcgcgc gctttgcgac ctgggccgag agcgaggagt cgcgccccag ggttacgacg
    17761 gcgcccgggt cgagcgtggt gccgaacgat gatgccaggc gggtgacgaa ctcgggggtg
    17821 agatccacgt tgaacttgcc gctcacgacg ccgcgacgga acacggtgcg aaggcccatg
    17881 gtctcgtaga tcaaggactg cgtgacgttg gcccctgact ctatggtctt gtgggggtag
    17941 accttgacct cgggggctat ggtcgccccc tcgccgacga tcacgtcgtc accgagcgcg
    18001 ctgcgctcca ggatgcgggc ccgcgcctgg acgtaagagt tgcggccgac gagggtgtct
    18061 accagctcgg cgccctcacc tatgtaggtc ccctcggcga tgacgctgcg ctctattgtg
    18121 gcgcccgccg agacgacgac gttgtcggcg aggaccgagt agggcccgat cctcgcgcct
    18181 tcgtggatgc gggtgttctc cccgatcacg acgggccctt cgagttcgtc gtcgtcgacc
    18241 tgcgccctgc gccccacgta gatgttctcc ctcaggcgcg tgcccggggg cctgacgccc
    18301 tctacgtgtc cgtcgagcac gtctcgctgg gcgctggcaa actgctccag ggtgcctata
    18361 tcctcccagt aatcctcggt gatatagccg tagagtgggc gtccggcctc gagtagtttg
    18421 gggaacagct ccttggagaa gtcgtactct ccttcttccg ggtcggggat ctcggcgatc
    18481 gccgaggact cgagcagata gatgccggtg tttacggtgt cggagaagac ctgcccccag
    18541 gccggcttct caaggaaacg ggagatgcgc ccgtcctcct cggttatgac gatgccgaaa
    18601 tccagggggt tctcgacgct cttgaggacc atcgtggcct cggagccttt ctcttcgtgg
    18661 aattcgacaa gagtgctcag gtcggcgtcc gtcagcgcgt cgccgcttat gatgagcagg
    18721 cgctcgccct cgaggccgag ttgctgctcg gccatcttca cggagcccgc ggtcccggcc
    18781 ggcgcgtcct cgacggagta gcgcatgttc acgttccaat cggaaccgtc gccgaagtag
    18841 tcccttatct cgtccggcaa gaactgcaag gtgacggcga tatccgtgaa gccgtggcgc
    18901 tcgaggagct tcacgatgtg ctccatgcag ggggcgttga cgatggggat catcggcttg
    18961 ggctggtcgc tggtcagggg cctgagacgc gttccctgcc ccccagccat gatgatcgcc
    19021 ttcatcgtag gtcactccat gccgggacat accgccaacg cctcaacccg gccgcaacgt
    19081 cgaacatgtt ctctcctctg tagctcccga tccttccctt ctcgcatgag tttatcagca
    19141 cgctgccttc ggcagctagt cagcacgctc cagcctacgg ctgtcgcttg tcagcacgcc
    19201 gcctcgctgc gctctgcggc tctcagcatt tcagctactc gtcccatagc caccctacaa
    19261 gcgcaaaacc tacgccgccc caagtagcgg ttctctcatg ctgccaagcg aggcaaaaga
    19321 ggaagaagct gatctgatga ctagctctct ggcgttcgaa aagaggtgca cctcttttcg
    19381 aactacctgc tgctcttgac cttgattctg acgctgacgc cccagagtcc gtacctctcc
    19441 cggagggcgt tttctacgta gcgcagatag ttctctggca ggtctttggg cctggtcgcg
    19501 aagagcgtga acctgggtgg cgccgcgccc gtctgggtgg cgtagcggag cttgacgccc
    19561 ttcggcggcg cggtgcgcgc tacgacgtcg cccacgaact ctgcaacctc gtgggtcggc
    19621 acccgcagcc tgtatgcccg cgctactccg gccgccagtg gcagaacctc gtccaccccc
    19681 gcgccggtga gggcagagat gtggacgaag ggcggctcga ggtcggtgag gcgggcggag
    19741 atgaggcctt ctatctccct gagcctctgg gggggtacga ggtcccgctt gttgaccacg
    19801 accccgcacg aagagccggc ttcttctatc tcgcgggcga tcctgaggtc acccgccacg
    19861 atgccgtccg tggcgtccac caagagcaga gagacgtcgg agcgcctaat ggcctctgag
    19921 gttcgcaagg acgagtagta aggcaccccg cgagcccgcc tggcgccctt gctcacgccc
    19981 gccgtatcga gcaagagata gcgttcgggc tctccctcta catcgagcgc gatctcggcc
    20041 gcgacggcgt cggtcgtcgt gccggcctcc tccgagacga cggcacggtc cgtgccgagc
    20101 agacgattga ggagcgtgct cttgcccacg ttcggcctcc cgacgatggc gagcgccggc
    20161 ggctccactc cgccctccgg ttgtgactcg ggaagcaggg agacgactac gtcgaggagg
    20221 tccccgacac cgagtccgtg caccgcggag atcgcatgcg gctctccctc accgagggag
    20281 tagaagcggt gacgctcggt gtcgtcggcg gggttatcca gcttgttgac ggcgaggacg
    20341 gccttgcccc gcgctcgcct gagctccctg gcgataacgg cgtcggcctc ggtcggcccc
    20401 agcctggcgt cggtgaggtg cacaaccaca tcggcctcct cgagcgccac ccttgcctgg
    20461 agcgtcacga gggcctcgag gcctaccctc gcgcgcggct ccatcccgcc ggtgtcaacc
    20521 agcgtgaact ccctgcccgc ccactcggcc cggttgtacg ccctgtcgcg cgtggtaccc
    20581 ggtctctccg agaccacggc accgcggcgg cgcgtgatcc tgtttaccag cgagctcttc
    20641 cccacgttgg gggccccgac gacggcgact accgggaggc ggctcacagc gacccctgcg
    20701 cgcgtctttg gggcagggcg tagatggtgt gcatgatctc atccgccgcc gcgcggtatg
    20761 ccgccccacc cctaatggcc gggtccaggt gcacgggttc gcccacgaag atctcgagcc
    20821 gccctccgcg cagccgggtg aggggtcctg gtagcctgcc cacgtgaacg ggcagtacac
    20881 gagcgccgct gcggagtgcg agtacgacta ccccggtttt ggcctccctt gtggctccgg
    20941 cgcccctgtg ggttccttcg gggaagatcc ccaacgccca accctcggcc aggaaggcca
    21001 gggcggcgcg tatcgcagcc cttcctccgc cctcgcggtc cacggggaac gacccgataa
    21061 actcgaagaa cctcctgaaa gggaagacga agagctgctt cttggccatc cactgcaacc
    21121 gacgggggac ggccacacac actaggaccg ggtcggcata ctggctgtgg ttggaggcca
    21181 ctatcagggg tccttcggag ggcaccctct cgcccccgtg caccctgcag gcgaacaaga
    21241 ggcggcacag cagggtcacc gcccgccgga acagacggta ccttcgcgtc atctcgcccg
    21301 gtttgcgcct cccctcgaag agcatcgctc tcctcacgtt ctcccgcgaa ggtccgtggc
    21361 gaggtcgagg accttcgcta tgacctcttc gaggctcaag gaggtggtgt ccaagaccac
    21421 ggcgtccgcg gcgggcttga ggggtgagat ctcccgctca gagtcctggc ggtcgcgcct
    21481 ggctatggcg gcccttatcc gatccagctc cccctccctg cccgtctgcg aagcccgccg
    21541 gcgcgcccgc tcttctggag cagcggatag gtaaacctta agctcagcgt tagggaggac
    21601 gacggtgccg atgtctcttc cttcgacgac ggccccgccc ctggccttag ccttcccggc
    21661 ggcggccctt tgcacgggga gcaagacctc tctcaggggg gcatgggcgg agaccttcga
    21721 agccgcggcg gagacttcgg gagagcggag atcgcgctcc ctgacccgcc ccccgtcgat
    21781 actcgcacct tccggatcca gggccaccct gcgcgcgagc gcagcgagcc cctccgcgtc
    21841 tgtgaggtcg acgccctctc gcagggccag aagcgccgcg gccctgtatg ccgcgcccgt
    21901 gttgaggttc accaccccga ggcgccccgc aactgcccgc gcgaccgagc tcttcccgct
    21961 ccccgcgggc ccgtcaatgg ctacgagaat gcctcccatg gacccaggaa gtatagccga
    22021 aagcccagcc ggttcacccc aacgatcgca aagccccaaa gtagcccgga aacgtcttgc
    22081 tgacgcaccc agggtccccg atagcgatgc ccggcgcaac cagccccgtt accgcaaaag
    22141 ccatcgccat cctgtggtcg ccgtaggtcc gtacgacccc gggccgtatc ttcccaggta
    22201 ttatgcggat agatgatggg gttgttcgag tttttatgcc gaggcggttc agttcggttt
    22261 ggacggcgcg gaggcggtcg gtctcctgca ggcgggtatg ggctacgttc tttatggtgg
    22321 tagggtcctg ggcgaagggt gcgatggcgg agagggtgat catggtgtcg gagaacgcgt
    22381 tcatgtcgac ttcgacgccc cgtagacggt ctggcccggt cacctgggtg gcgctggctg
    22441 tgatctcgac cctacagccc atgtcgcgca ggacttcggc gaagcgcagg tctccttgcg
    22501 gggaggagga tcccaggcct gggatcctca cgcgcccgcc cgttgcggcc gccgccgcca
    22561 tgaagtagga cgcccccgag gcgtcgggct ccacttcgta ctcccgcgag cggtacgtgg
    22621 cggggtcgac ggcgaagcgg ccgtttgttt ctacgacctc cacgccgaag gcccgcataa
    22681 gggccgccgt gataccgacg tagggccatt ctttcctgcc ctcggggtac agcttgacgg
    22741 gagcctccgc gtagggagcc gccatgagca gcccgctgat aaattggctg ctcttgctcg
    22801 ccgagaagcg cgcctcccca ccccggaggc cgcctccctt gatggtgagg gggaactttc
    22861 cgtcctcccc ggcgtaggcc accgcggcgc cgagctgcct catcgcatct acgagacccg
    22921 cgaccggacg ctcgcgcatc ctgggtaccc cgtccacggt gtagggtccc ctgccgaggg
    22981 cgagcacggg cggcaggaaa cgggccaccg tgccggcgtt gccgacgaag aggtctacgc
    23041 cggacgcgtc gaacccaccg ccctggccgc ttacgtagac ttcccccttc tctctttcgg
    23101 cgctgacgcc gatgccgagt cggacgaggg cgttcatgag ccagaaggaa tcgtccgaga
    23161 agaggggatt gaggatcctc gagcttcccg ctgcgagggc ggcgatgatg agggcgcggt
    23221 tggtgacgct cttcgaacca gggacccgta tcgtcgcgtt cggcgggtct tttagcggag
    23281 ttacttcgag ttgcgcgggg agggtcggac gtacgtcttc gacggtgaaa tccaccccgg
    23341 gcacccccgc gaggttcttc accgtaaggc ctcgagttca gaggggctga gggctcggta
    23401 ttcgccctcc gggaggtctc cgagggcgac gggacctacc cgcgtcctgt gcagggagat
    23461 caaggccaga cccacggcca tacaggctct acgtattatg cggttacgtc cctcgtggat
    23521 ggtaaggtcg agcgtggtgg tctccctgga gcggctgagg ttcgtgatct cgggcgggtg
    23581 catcttgccg tcatcgaggg tggggccggc ggccagttgt tctatggctt ctgtaggcgc
    23641 ggggttttcg agggtgaggc ggtagcgttt ttcgatctcc gaggaagggt gggtgatgcg
    23701 gtgggccagg ccgccgtcat ttgtgaggat gagcagtccc gtcgtggttg cgtcgaggcg
    23761 gccgacgggg accagtccgg gcacttcggg catgaggtgg gcgacggtag gtcggccgcg
    23821 atcgtcgttc atggtggtca gatatcccgt cggtttgttg agggcgaggt agacgcaggc
    23881 ttcagggagg cggacggggt ggccgtcgag ttcgatctcg tccccgggcg ttaccctcgc
    23941 gccgagggtc gccgtctcgc cgttcagcgt cacgcggccg gccagtatag gcgcctcggc
    24001 cttgcggcgg gagggtgccg cgccggcgcg ggcgaggaac gcctgcaggc gcatcagggt
    24061 tcccctgcat ccggctgacc cgtgccggac gaagccagcc gttcgcgcac gcgcgagagc
    24121 tcttcttgcg tcaccagcga acctgcggga gggaaatcat ccggggagga ggcgcccgtg
    24181 gcggagagaa actcctctgt cacgccgagg agtgcgggcc agcccggcgc ttcgcggtcc
    24241 gtgccgactt cggccagcag tccgcgttcg atcaggctgc gtacgacggc gtctgagttt
    24301 acgccgcgca cgcgggagac ggcgccgcgg gagagcggtc ccaggtagag cgcgcaggcc
    24361 aggacttcgt gggccgcgcc ggagagcggc gcgggacggg cctccctgcg aaagcgctcc
    24421 acggcggcgg agcaggccgg attcgtcgag agcaggtgtc ctccggcgac atctctaagc
    24481 acgatgcccg aagcttcggg ggcgtagcgc tcccgcaagc gggcgagggc gcttttcagc
    24541 tcgccatcag agagacccgt cgccgagcgg aggtccgagg cgctgagcgg gctcgcgctc
    24601 accaggagga cggcttcgac caggtgcgcc ggggagggga gcttgcgttg ggggtcgatc
    24661 aacgtgacgg ctccagggtc aagggtccca ggggttcggt ttggacgagc ctgaggcttc
    24721 cctcctgggc gagcgagagc gcggcggcga acgttacggc tttgttcaag cggtccatgt
    24781 cgctcgtgag gctctcgtag gagatggttc ctttggccag ggccgtgcgg atgatcccgg
    24841 ccagttgctg gagggtcacc gtgatggggc cgaggtgaga gaccggcggc tcgagaatgc
    24901 gggagaacgc ccgtcgcgcc gcgagcgaga ggcggttggg atctacgtgc aagcttcccg
    24961 gtctgggagg catcgtgtgt cccgaggcgt aatggcctgc gttggaggcg aagcggtctt
    25021 tcaggtgttc ggccgcgcgc cggatcttca ggtactcggt gagacgctcg gccagctctt
    25081 ccggagagag ggtctcgtcc tcgacgcctt ccgggtctgc gtcgaccgcc ggtgttagcg
    25141 ttcggctctt cagcaagacg agggccgcgg cagagccggc gaagtccgtg tcgcgctcca
    25201 ggccgttcgc cgcgcctggc cggcgggaga ggaggtagga ggcgaccagc tcgcgcagtg
    25261 ggacctcgaa gatctccaat tcgtccttca ggatgagggc cagcagccac tcgtacggac
    25321 ccgagtatac gtcgagctcg acggtaaagt ccgagagtga gcgcccgagg ggttcggggg
    25381 tcatcttgcc tccgaatgga gcggcaagac ggaggagagc tggttgtact ccacgcccgt
    25441 atcgcgggcg aggacggggt cgacgacccg cccttcggcg acggcgacgc cccttgccag
    25501 tccctcgtcg gcccccagcg catccgagag gccgaccccg gcgagcttgc ggaggtaggg
    25561 caagagtgcg ttgctgaggg ccctcgtggc cgtgacgggc accgaggccg gcacgttctt
    25621 cacgcagtaa tgcgtaaccc cgtactcgac gaaggtgggg tcggcgaggg tcgtaggacg
    25681 agaggtcgcg acacacccgc cgtagtccac gtccacgtcg acgatcacgc tccgctcctt
    25741 catctccgag acggtctcgc ggtccacgag ggccggagtg cgggtgccca cgacgtggac
    25801 cgcccctatg aggaggtccg agcgcagcgc ggcgtccctg atccccaggc tgctcgccgc
    25861 cagggtcgtg gtgttgggca ggcgggtgtc ttcgatctgc ctgagtcggt cgaggtctct
    25921 gtctatgatg accacctcgg ccccgagtcc gctggccaca ctcgccgcac cggccccgac
    25981 gatcccggca cccaggatga ccacgcgtcc tggcctcacg cccgtcgagc cgccgaggag
    26041 tattccgcgc cccccgaagg gtcgttgcag gtagtgggcg cctatctgcg gggtgaggcg
    26101 gccggcgatc tcgctcatag gcgtgagcac gggaaggcgg ccacgagcgt ccctgatggt
    26161 ctccatggcg aaggcgatgc aacgagagtc gatgagggcc cgcatcaact ccaggttgac
    26221 cggcagggag aggaaggaga tgaggatgag atcctccctc aggtactcgt actctccttc
    26281 tacgggcccg tagaccttga ccactatctc ggagtcggca tagatcttcg ccgcgtcctt
    26341 tacgaggcgt gcgccggcct cctcgtagct ctcgtcgccg atgctcgacg cctccccagc
    26401 cccggcctcg acgaagaccc tgtgtcccgc gcgggtcaac tcgtacacgg cgtgcggggc
    26461 cagcgcgacc ctgctctctc cctgggtcct ctctgtggga accccgatcc tcaagggtag
    26521 cccccaagga gatccgcggg ggagaccgtg tacgacgact gcgttctcat gctcccgaag
    26581 ggtaccctgg atcttgcctg cgatccagcc gcgcggggac ctcagggggc cttccaagcc
    26641 gggtgggcct cgatgaagct ccgcagttcc tgttcggctc cgagtattcc gaggcccacc
    26701 gccccttccg gcaccggggt ccaagatggg gcgaagacga ccccgaaacc ggcggcccaa
    26761 aggtcgaggg ccagggcctg caaggcggtc gccggtgccg aggcaggggg cgtgacgcgg
    26821 gttgtcctaa tctccaaaac ggcgtcggag agcgcagccg aggtcgccag cgtacgcaag
    26881 acgttctcca gggccgagcc gaggtcccgt tcggagaggg cgagcatctc gcccaggatt
    26941 tcacgcttgc ccgtctcggg gtcaaccgac tcgaagacgg aacatctcgc ggtcccgggc
    27001 ggggcggcga gcgccggcgc cttcggcagg tcttctagcc cgttccagga gagcgtcgcc
    27061 gagaactcca gcaggcccgc cgtgcgggtg gcgacggtgc cggcggggtc gccggtcggg
    27121 aacacggtgg tgggccgcct ttcggcgctt atgaggcccc gcgtccggcc gacctccctc
    27181 agcgggtcgc cccctgttcg ggcctcaggg gctccgtttt cagtccggcc gtttctttga
    27241 gggtctcccc gcaggcgccc acgaagccgt agaacagcgg gtggggcctc aagggacggc
    27301 tcctgaactc cggatggaac tggctcccga tgtagaaggg gtggtcacct atctctgcaa
    27361 tctcgaccaa gtggccgtcg ggcgaggtgc ccgagaagac catacccgca tcggcgagcg
    27421 tgtccctgta ggcgttgttc acctcgtagc ggtggcggtg gcgctcgtgg acgaggcccg
    27481 atccgtagac ctcctcggcc agcgttccag ggatgatctg gcagggccag cgtccgaggc
    27541 gcatggtacc gcccatatcg actccgacct ggtcgggcat gatgtctatg accgggtgcg
    27601 gcgtatcctg ttcgaactcg gtggagttcg ccatctccag gcctgcgacg tttcgggcga
    27661 actcgatgac ctctatctgc atcccgaggc agagcccgag gtagggagtc ccgctctctc
    27721 tggcgtagcg cgccgcctcg atcttgccct cggcgcccct gtggccgaag cccggcagca
    27781 cgaggatgcc gtcgaccccc gagagcctct cctccgtgga aggtccgtcc aggagctgct
    27841 ccgagtcgac ccagtcgagc tccaccttca ggccgtgggc gccgccggcg tgggccaggg
    27901 cctccaccac ggagaggtag gcatcctgga gctttatgta cttgccgatt atcgcgaccc
    27961 tcaccgtctg agaggcctcg agcatgcggg agacgaggtg ccgccactcc ccgaggtcgg
    28021 cgtggggcgc gctcagcccg agcttgtcca gcaccaggtc gtcgagccct tcctcgtgca
    28081 aattgagggg gatcgcgtac aggttcgggg cgtcctcggc cgcgataacg gagtcgagct
    28141 ccgtgtccgc aaacagcgcg atcttcctcc ttatgtcgtc gcttatgggc ctgtccgcgc
    28201 ggcagatgag cacgtcgggg cttatgccga tctcgcgcag gcgctgagcg gagtgctggg
    28261 tcggcttggt cttcaattcg ccggcggcct cgatgtatgg tacgtaggag acgtggacgt
    28321 agagcacgtt cttgcgtccc acgtcgtttc ggaattgcct tatggcttcc aggaagggca
    28381 ggctctcgat gtcgcctacg gtgcccccga tctcggttat gacgatgtcc ttgtcctgcc
    28441 cgaggcgccc gatcctgttc ttgatctcat tggttatgtg cgggatcacc tgtacggtag
    28501 cccccaggta gtcgcccctg cgctcgcggg taatgacctc ccagtagacg ctacccgtgg
    28561 tcacgttgga gaggcgaccc aggttctcgt cgagaaagcg ctcgtagtga ccgaggtcca
    28621 ggtccgtctc cgccccgtct tcggtgacga agacctcgcc gtgctggtaa gggttcatcg
    28681 tcccagggtc cacgttgatg taagggtcga acttctggag aacgaccctg tagccccggc
    28741 tcttgaggag catacccagc gccgccgcgc tcgtgccctt gcctatggac gaaaccacgc
    28801 cgcccgtcac gaaaacatac ttggtctcac tcatctacac ccccgaagaa agaaccaacc
    28861 caacacccct accttcactg ccagtcggaa aaggggaaga gaatccggtg gggcgcggga
    28921 tcttcgcttc ccgcaaacct ctactccggc acacctgcat ggatctctat catacgactt
    28981 ctggcgccag aatgatacag gagatcaccc aacggtattc ccacggtgag atctggccca
    29041 aagtagctac tccgtcttcg gtcacgacgc tagtctttgg cgctacagac ttcgaagcgt
    29101 tgtgaacgcg agatatcctt gctaaggcgg ttattgtatg atgtcgaggg gtccaggagg
    29161 agctggtggt ccggaacatg agatggagac cgcgctggac aaaaggaacg aaaatcttgc
    29221 cccgtgcggc cacagccgct tcgaattgga ctttcgacgg ccttcgcttg aagtcggcgc
    29281 gggcgctcct ggccgttacc aaagacgaca tggccaattt gcagtgcgcg ctgccggccc
    29341 aggcgcgggc cccaggtctg cgggtagtgc tgcgcctctt cgaccccgac ctcgccgcga
    29401 gagtggagcg caccacgggc atctatctct cccgcagcgt gtccgcgctc gcggcccccg
    29461 ccttcgtagc cgccatcttc ggccgaaagc ccgaggccgt gctccccatc ggcaccgagg
    29521 tcctgcagat cgtcgacctg aaggtagaga aagcgtccga cgtaggcacc ctggaggacc
    29581 gctgccaggc gcgtgtgctg gcggtcgggg cgacagcatt cccggtaccg gaactcacgg
    29641 tctctcctgg cgacgagatg cgggtggtcg gcaccaaccg ggggttgatg gagctcgccc
    29701 gccaggtcgc cccttcggcg ttgacggcct ggagcgacgt gaacgaacac cgcggagaga
    29761 ccgcaccgta aacaggggga gctgcgattc gctgatgaag aaccacgcct caggcatagt
    29821 cccccatctc ggtcacgttt cgccgccaag gcggagggcg attatactgc ctccctgaaa
    29881 ctctgcctcc actacgccct tgaggcggcc gggggaaccg gcgtccgccg gcatcctgac
    29941 cgtccacgcg accccgttgc gcgcgacctg cttcctggtc ggatcgacgc ggacgccccg
    30001 tgccagaatg tctgctacga aggtacgcac ctcgcgtgtc cctttgtgga taccatcgtt
    30061 tgcgaacggc gccgaggaga agatctgtgc gttatcggcg aagaagccca ctatggcggc
    30121 tgtgtcccgg ctcttccagg cgtcctcgaa ggccagcgcc gcgaggaact cctccgccct
    30181 cgcttcgccg tgggacatca aatcccggat aaagtgcggg tcgcggttca gcttggaggc
    30241 cgtaccgagg aagcgcgaga agcgggacct ggagagctcg atcacgcgca ccacgatctg
    30301 cttgtacttg ccgtcgctga ccagatgacc ctcctccagc atctggtcga tcctctcgat
    30361 gaatccgagc tcctgatgca gtgagaggtt gcccgagagc tcgttgcgcc ggtccgctat
    30421 ctcggcgatc gtcctaggct cggattcgcg ctccttgggg tttatctgga tcacccagat
    30481 ctcatccggc cgctcgtcgg tcagctccct taccggggga ttctgcgaga agagcccgtc
    30541 ccagtaggtt cccccgtcat cgaggcacac ggagcgaaag agtgtcggga tggccgccga
    30601 agcgaggatg gtctttgcgg agatcgcgtc ccgcctgctg ttgaaggtcc gaaactcacc
    30661 gctgagcacg tccacggccc cgaccagcaa cacggggtaa gacccttccg gctgcacacg
    30721 tacgtttcgg aagtcgatcc tccgctctaa cagcctcctg aattcctcga gtcccaagtc
    30781 ggagaaatag ttatcgtagg gactgatggc cggtatcccg acgaagttct gcactttgcc
    30841 tgcccagacg gtccagttgt tgaggacctg ctccaggggc gtcgttgcag agttgtcgcg
    30901 ccagaatgca tcgagatcct caaccgcgcc agcggcgtcg cccctcaaaa gtttgtgcca
    30961 ggccagcagg gcgcacaccg ccccgcccga ggtgccgctc aggccgacca cttcgtaccc
    31021 tgccaggttc tgcgcacgca gaagtctgcc cag
//
LOCUS       DATMVF010000090        10902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_198528, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000090 DATMVF010000000
VERSION     DATMVF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198528"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VN178_04140"
     CDS             <1..251
                     /locus_tag="VN178_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWS80192.1"
                     /translation="ADASVRAWEALAYSTLLVASFGFWAWQRGVSQVGANRVLIYQYL
                     ITLVGVAAGVLLLGESLTANKVLGGAIILLGVYLARRQ"
     gene            927..1688
                     /locus_tag="VN178_04145"
     CDS             927..1688
                     /locus_tag="VN178_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWS80193.1"
                     /translation="MRENHLREVWREGRTAVNGWLHVPSSFSAEVMAHAGWDSLTIDM
                     QHGPVDYGSLVPMLQAISTTDTVPVVRVLWNDPGLIMRVLDAGCYAVICPMINTREEA
                     EAFVGACRYPPEGYRSFGPYRATLYGGQDYTDHANEAVVTMAMIETQQALDNLDQILA
                     VEGLDAVFIGPSDLGQSLGHGPGMDRGEPEVVEAIERVLTAAREHGLVAGIFTGSPEY
                     ASRMAGKGFQFVTISSDVRLMASAAAGAMAAFDSE"
     gene            1750..2007
                     /locus_tag="VN178_04150"
     CDS             1750..2007
                     /locus_tag="VN178_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWS80194.1"
                     /translation="MAERETTVGPEEGIHGRPAARFVKAAKGFSSQVVVVKGDREVNA
                     KSVLKITGLARKGEKVTIRAEGEDAEQAVQTLAELIAADEH"
     gene            2023..3642
                     /gene="ptsP"
                     /locus_tag="VN178_04155"
     CDS             2023..3642
                     /gene="ptsP"
                     /locus_tag="VN178_04155"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWS80195.1"
                     /translation="MKLTGIGASEGVAVGPAFVPATGRPDPERYRITEDEVDAELQRF
                     EEAVEAVADDLSETGERLRAAGSEEEAGILDFHIEMAEDPELASEVEERIRNLESPEA
                     AVLAVGEEYAAQYAAMDDEYLAARGDDVLDVTGQISARLMGRAASSFEALETPSVVIS
                     HTLAPSDTARIQKGMALGFVTAEGSRTSHVSIMARSMGIPAVVGVGPALEKVLDAKTV
                     AVDGAEGYAISDPDAETVADFERMSGALAEERAALDEYRHVEAVTSEGLRIEVAANLG
                     SSSEAEGALEWGAEGVGLFRTEFLFMERDELPSEDEQYEAYSAVARAFGEKPVIVRTL
                     DVGGDKNLPGVDQPQEENPFLGWRGIRMSLDTPGLFQPQLRAILRAAANGNLKVMFPM
                     VADVGELEAAKETLLKCRRELESEGAKIGEVETGVMIETPAAAVCAAELAEEAAFFSI
                     GTNDLVQYTLAADRGNERLRRLQNADHPAVLALIRRACEAANAAGIPVGVCGEAAGEP
                     AMIPKLVELGVTELSMAAPQIPRAKKVVSEL"
     gene            3639..4379
                     /gene="nagB"
                     /locus_tag="VN178_04160"
     CDS             3639..4379
                     /gene="nagB"
                     /locus_tag="VN178_04160"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HWS80196.1"
                     /translation="MRARVFPDYEGISAAVAYVVAGRLEEEPSTVFMLPTGITPLGMY
                     RRLVEMHRSGRLSFANATFFNLDEYLGLAPDHRASYHVYMKEHFYGLIDADPARVFVP
                     DGTAPDPEAECRRYEAAIEEAGGIDVCVLGIGRNGHIGFNEPGAPFDSRTRVVELSES
                     TRRVNASDFEADRAPERAITAGMATIFDSKSVLLLASGTNKAGAVAAAVEDDVSESMP
                     ASMLRSHPSAVLFLDEAAASDLGHGVAG"
     gene            4516..5550
                     /locus_tag="VN178_04165"
     CDS             4516..5550
                     /locus_tag="VN178_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80197.1"
                     /translation="MSAFIERITGSQVGGATERSIRLSLSIIRLGPAIILLLLVVAMT
                     LLSPVFLTGANISNVVVQTSVLAVLAIGQLFVILVAGIDLSVGSVLGLSTVTGAIVYT
                     ATSGAGGLLALLVILLTGVVFGWINGFLYVKGKMPHPFIPTLASLYAARGLALVISDG
                     RPIPGMSPIVVFLGSGYIGPIPMPVVVVAVLAALAIIVTRRLKWGRWIYAVGGDKEAA
                     RRTGIPVDRVLISVFVISGLMAGFAGVLTAGRTDAGFPTAGELEELTAIAAVIIGGAS
                     FFGGRGTVINVIVGALIIGVIQNGLNLLNVNVYYQLVATGVIVVIAVELDVLRRRLEQ
                     RFRTLQAREA"
     gene            5554..6399
                     /locus_tag="VN178_04170"
     CDS             5554..6399
                     /locus_tag="VN178_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS80198.1"
                     /translation="METEQAQQPREENGGPVLEIRDLTKRFGATLALNSVSLTLGRNE
                     VLALLGDNGAGKSTLIKCLSGVHEPDEGQILIDGKEVQITSPMQARSLGVETVYQDLA
                     LFDNLDVVGNFYAGREPTTPGWLGNWGWVKDKDMAREADELMSRLEINLPGADVETGL
                     MSGGQRQAVASARAMAFGRKIVILDEPTSALGARESRNVLRLIQDAPSQGVSVIVISH
                     NLEHVMQVADRAVVLRQGNVVGEAEPHQENHERLVSMIVGGADEPADDEETEASAEED
                     IERDG"
     gene            6392..7372
                     /locus_tag="VN178_04175"
     CDS             6392..7372
                     /locus_tag="VN178_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWS80199.1"
                     /translation="MGRRLALPAPLFFLILLLVTGCGGQLAPSYLTGEMKVGFVPKTL
                     DQEFWVTTEKGARAGGREADATVLVQAPTGELAVDEQIAIVENLLTQNIDALVVAPSD
                     SILMKPVLERASEEVPVILLDTDIPKWKPKDSYVGTDNFAAGLAAGRYLERQMNGKGS
                     IGLITGIPGSVPGDQREAGMRKAIENTEIKVVGEVAADFDRLKAVGAMEDLLQTHPDL
                     DAVFATNDQMALGAIEALQTYRSDAILVGVDGAVEATQAIIGGDMDATVAQNPYAMGK
                     VGVQQATKAANGESVDPRINTGLTLVTKKNAPSYLKVREEQLGSLLGVEE"
     gene            7374..8369
                     /locus_tag="VN178_04180"
     CDS             7374..8369
                     /locus_tag="VN178_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS80200.1"
                     /translation="MKVGLLGAGRIGALHAGVLARDPTVDEILIGDADAQRAEEVAQE
                     IGGEAGRIEAVLASGPDAVVIAASTPAHAPLIRAAVEAGIPAFCEKPIALGYEETVEI
                     AHEVEDSGATLQIGFQRRFDAGYAEARRLIDSGALGTIYSLRLATHDPDPPPEEYIPD
                     SGGIFRDLHIHDFDILRWLTGGEVEEVYATGSVRNFDFFATHGDVDTSAALIRMQDGA
                     VAVLTGGRHDPLGYDVRAEIFGSRDSVAVGMDRRTPLRSVEPGIPASEKGAYPNFQER
                     FLDAYTAEIEHFLSLVRGEADNPCTATDALEALRIAMAADLSLTEHRPVSPSEVG"
     gene            8366..9238
                     /locus_tag="VN178_04185"
     CDS             8366..9238
                     /locus_tag="VN178_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWS80201.1"
                     /translation="MMSRVAGAPISWGVCEVPGWGYQMDAERVLDEAAGLGLTALEAG
                     PDGFLPGEPDVMASLLSQHGLELVGGFVPVVLHRQEARDSELASVERQAKLFAAAGAG
                     VLVIAAATGEEGYEESAGLKDGEWDELFATLAMVEEIGTKHGLSVVLHPHYGTEIESP
                     EHVQRFLEGCDTALCLDTGHSMVGGGDPVALAESAATRIRHVHLKDVDQDLAERVVSG
                     TLPYEQAVRDGLYMPLGDGDVDVRRVLEVLDGAGYDRWYVLEQDIMLSADPGYGPAAE
                     VGRSLAYLKGVLEE"
     gene            9429..10373
                     /gene="iolC"
                     /locus_tag="VN178_04190"
     CDS             9429..10373
                     /gene="iolC"
                     /locus_tag="VN178_04190"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HWS80202.1"
                     /translation="MNPEVITMGRVSVDLYPEQIGVTLAEVETFAKSLGGSATNVAVA
                     AARLGHPSALITKAGDDGFGQYVRTALEGFGVDTTWVSTDPELRTPLAFCEIHPPDDF
                     PILFYREPKAPDMNLTVEDLDFDAISAVPLFWITGTGLSDEPSRTATLAALEHRPTGI
                     TVFDLDYRPTLWASKEEAAHCYREALRHATVAVGNQSEVEVAVGTGDPFEASEALLDL
                     GIELAIVKRGPEGVLARTKDGVAEAPPIEIEIVNGLGAGDAFGGALCHALLSGWDAER
                     TIRYANAAGAIVASRLACADDMPTRDEVEQLLEKSSNA"
     gene            10583..>10902
                     /locus_tag="VN178_04195"
     CDS             10583..>10902
                     /locus_tag="VN178_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWS80203.1"
                     /translation="MTTVRLTMAQALVRFLTEQYVERDGIEHRFFAGCFGIFGHGNVA
                     GVGQALQQYAGGLPYYQARNEQAMVHTASAYARQKDRLQAFACTTSIGPGATNMVTGA
                     ALAT"
BASE COUNT         2018 a         3466 c         3579 g         1839 t
ORIGIN      
        1 gggccgatgc cagcgtaagg gcctgggagg ccctggcgta ctctacgctc ctcgtggcgt
       61 ccttcgggtt ctgggcctgg cagaggggcg tctcgcaggt cggggcaaac cgcgtgctca
      121 tctaccagta cctgatcacg ctcgtcggcg tggcagcggg cgtgctgcta ctcggggaga
      181 gcctcacagc gaacaaggtt ctcggcggcg ccatcatcct cctgggcgtc tacctcgcca
      241 ggcggcagta ggcatcctgg cgggccaccc cgcccggctc tgcgaagaac cgacgatccc
      301 cagcgactct tagcccgttc ttagaatcgc cgtccataat gcgcctgttc tatactgcgc
      361 ctattcgggg ctcggcgatt gtcccaacaa tcatagactt cctttcccga gccccgaata
      421 ggcgcgtacc cgtgtatacg ggcgtgttct gcaaatggac cacttcggcg cccgtgcgtc
      481 gctcttccat ccttaccgaa tcgcttacga gttgggtggc ttactctggt ctggatggca
      541 gccgctcctg cgccttcccg cgaagttctc ctttcccacg taacggttga atcatagtct
      601 cctcgagcat ccggtacatc cttcaaatga gtgatttttg gtgcatacta cagttgcgag
      661 cggggtgcta gtggcattat cggcttgttc aacgaagggc cggcgcgtag ggcaacctcg
      721 gtcttctcct tgccattacg catcgtatct ggcggcgacg cgagtgcgca cggcccgtgc
      781 gctggtatcc tttggagcgt ggcggggatc tcggagaact gaccggtatc gcccgctatt
      841 gagacgggcc ggcggaggtc gccgccgggt cagggacgga tcgatgcacg cgccgcgaca
      901 ctggggcgac gggagggaga ccagggatga gggagaacca ccttagagag gtctggcggg
      961 aaggccgtac cgcggtgaac gggtggcttc acgttccgag ttccttctcc gccgaggtaa
     1021 tggcgcacgc gggatgggac agtttgacca tagatatgca gcacggaccc gtggactacg
     1081 gaagcctcgt gccgatgctc caggccatct cgacgacgga taccgtgccc gtcgttaggg
     1141 tgctgtggaa cgatcccggc ctcatcatgc gcgtgctcga cgccgggtgt tacgcggtga
     1201 tctgcccgat gataaacacc cgcgaggaag ccgaagcctt cgtcggggcc tgtcgctacc
     1261 ctcccgaggg ctaccgcagc ttcggcccgt acagggcaac cctctacggt ggccaggact
     1321 acaccgacca cgcgaacgag gccgtcgtga cgatggccat gatcgagacg cagcaggcgc
     1381 tcgacaacct ggaccaaatc ctggccgtcg agggcctgga cgccgtcttt atcgggccct
     1441 ccgacctcgg ccagagcctc gggcatggac caggcatgga ccgcggcgaa ccggaggtgg
     1501 tcgaagcaat cgaaagggta cttaccgcag cgcgcgagca cggtctcgtc gcggggatct
     1561 tcactggctc tcccgagtac gcctcgcgga tggcagggaa gggcttccag ttcgtcacca
     1621 tctcttcgga cgtgcgtctc atggcctcgg cggcggccgg agccatggcc gcttttgata
     1681 gcgagtgagg gtatcatggg gcaggtatgc cgggtcttgt agagagcgct ggtgtgagag
     1741 gagtttttcg tggcggagag agagacgacg gtagggcctg aggaggggat ccacggccgc
     1801 ccggcggcgc ggttcgtaaa ggcggcgaaa ggattctcct cgcaggtcgt tgtcgtgaag
     1861 ggcgatcggg aagtaaacgc caagagcgtc ctgaagataa ccgggctcgc cagaaagggc
     1921 gagaaggtca ccatccgcgc cgagggcgag gacgccgaac aggccgtaca gaccctggct
     1981 gagctcatag cggcggacga acactagatc tggaccgcgc gagtgaagct gaccggcata
     2041 ggagcgtcgg aaggagtggc cgttggcccg gcgttcgtgc cggccacggg cagaccagac
     2101 cccgagcggt accggataac ggaggacgag gtagatgccg aactccaaag gttcgaagag
     2161 gccgtcgaag ccgtcgccga cgacctctcg gagaccggtg agagactgcg tgcagcgggg
     2221 agcgaagagg aagccggtat tctcgacttc cacatagaga tggccgaaga cccggagctt
     2281 gcttcggagg tcgaggagcg cataaggaac ctggagagtc ccgaggcggc ggtgcttgcc
     2341 gtcggtgagg agtacgccgc acagtacgcg gcgatggatg acgagtacct ggccgcaagg
     2401 ggcgacgacg tgctcgacgt tacgggccag atctccgccc ggctgatggg ccgcgccgcc
     2461 tcgtcattcg aagccctcga gacgccctcc gtcgtgatct cccatactct cgcaccatcg
     2521 gacacggcgc gcatccaaaa ggggatggcg ctcgggttcg tcaccgccga aggttcgagg
     2581 acctcgcacg tctccatcat ggcccggtcc atgggcatcc ccgccgtcgt cggcgtcgga
     2641 cccgccctgg agaaggtctt ggacgccaaa actgtcgcgg tcgacggggc agagggctac
     2701 gcgatctcgg atcccgacgc ggagactgtc gcagatttcg agaggatgtc tggggcactc
     2761 gccgaagagc gtgctgccct cgacgagtac aggcatgtcg aagcggtcac cagcgaaggg
     2821 ctgcgcatcg aggtcgccgc gaacctcggc tcgtcctctg aggccgaggg cgcgctggag
     2881 tggggcgccg aaggcgtcgg gctcttccgc accgagttcc tgttcatgga gcgtgatgag
     2941 ctcccttccg aagatgagca atacgaggcg tacagcgccg tcgcccgcgc gttcggcgag
     3001 aagccagtca tcgttcgtac gctggatgtg ggaggcgaca agaacctgcc cggcgtggac
     3061 cagccccaag aagagaaccc tttcctcggc tggcgcggaa taaggatgag cctggataca
     3121 cccgggctct tccaacccca gctgcgtgcc atcctgcgcg ccgctgccaa cggcaacctc
     3181 aaggtcatgt tcccgatggt cgctgacgtc ggagagttgg aagccgcaaa ggagacacta
     3241 ctgaagtgcc gaagggagct cgagtcggaa ggggcgaaga tcggcgaggt cgagacggga
     3301 gtgatgatcg agaccccggc ggcagcggtc tgcgccgcgg agctcgccga ggaggcggcc
     3361 ttcttctcca tagggacgaa cgatctcgtc cagtacaccc tggccgcgga caggggcaac
     3421 gaacggctgc gacggcttca gaacgcggac caccccgcgg ttcttgccct gatacgtcgg
     3481 gcctgcgaag cagcaaacgc ggccggcatt ccggtcggcg tatgcggcga ggcggccggt
     3541 gaacccgcga tgatcccgaa actcgtcgag ctcggcgtta cggagttgag catggccgcc
     3601 ccccagatac caagggccaa gaaggtcgtc tcagagctgt gagggcgcgc gtcttccccg
     3661 attacgaggg catcagcgcg gccgtggcgt acgtcgtcgc ggggagattg gaagaagagc
     3721 cttccacggt ttttatgctg ccaacaggca tcaccccact cgggatgtac agacggctcg
     3781 tcgagatgca ccgctcggga aggctctctt tcgcgaacgc gaccttcttc aacctcgacg
     3841 agtaccttgg gctggcgccg gaccacaggg cgagctacca cgtgtacatg aaggagcatt
     3901 tctacgggct catcgacgcc gaccctgcgc gggtcttcgt ccccgacggg acagcaccag
     3961 atcccgaggc ggagtgtcgg cgctacgagg cggccataga ggaggcaggt gggatcgacg
     4021 tgtgcgtgct cgggatcggg cgcaacggtc acatagggtt caacgagcca ggagcgcctt
     4081 tcgattcccg cacccgcgtc gtggagctct ccgagagcac ccgcagggtc aacgcctcag
     4141 acttcgaagc cgaccgcgct ccggagcgtg cgatcacggc cggcatggct accatcttcg
     4201 actcgaagtc ggtcctgctc ctcgcctcgg gcacgaacaa ggcaggggcc gtggccgccg
     4261 ccgtggaaga cgacgtctcc gagagcatgc cggcctcgat gctgaggagt caccccagcg
     4321 ccgtactgtt tctcgacgaa gcagcagcct cggacctcgg gcacggcgtc gccggctaga
     4381 ccgcgcgcct tctcctccag aagccgttga cgtggggtgg ggactccagt agcatcgtca
     4441 caagcggtgg attccagagc ggcgaggggg tcggtacggc gaagcgcact tggccttctc
     4501 tggaaaaggg gagagatgag cgcgtttatc gagaggatca cgggtagcca ggtaggcggc
     4561 gccaccgaaa gatccatacg cctgagctta tctattataa ggttgggtcc cgctatcatc
     4621 ctgctgctcc tcgtcgtggc catgacgctc ctcagtcccg tgttcctgac gggggcgaac
     4681 atctccaacg tcgtcgtcca gacttcggtc ctcgccgtgc tcgccatcgg ccagctcttc
     4741 gtgatcctgg tggccggcat cgacctctcg gtcggctccg ttctcggcct ttccaccgtt
     4801 acgggggcta tcgtgtacac ggcgacctcg ggcgcgggcg ggctgctagc gctcttggtc
     4861 atcctcctta ccggcgtggt tttcggttgg atcaacgggt ttctctacgt gaagggcaag
     4921 atgccccatc cctttatccc gaccctcgct tcgctgtacg cggccagggg tctcgcgctc
     4981 gtgatctccg acggccgtcc catccccggc atgtccccga tcgtcgtctt cctgggcagc
     5041 ggatacatcg ggcccatccc catgcccgtc gtcgtggtcg ccgtcctggc cgcgctcgcg
     5101 atcatcgtga cccggcgcct gaagtgggga cgttggatct acgccgtagg cggcgacaag
     5161 gaggctgccc gcaggaccgg tatacccgtg gacagggtcc tcatctcggt cttcgtcatc
     5221 agcggtctca tggcagggtt cgccggggtg ctcacggcgg gcaggaccga cgcgggcttc
     5281 ccgactgcgg gcgagctcga ggagcttacc gccatcgccg cggtcatcat cggcggcgcg
     5341 agcttcttcg gtggcagggg gacggtcatc aacgtaatcg tcggcgcgct catcatcggc
     5401 gtcatccaga acggcctgaa cctccttaac gtcaacgtct actaccagct cgtggcgacg
     5461 ggcgtgatcg tcgtaatagc ggtcgagctc gacgtgctaa ggcgcaggct cgagcaacgc
     5521 ttccgtacac tccaggccag ggaggcatga gagatggaga ccgagcaggc acagcagccg
     5581 cgtgaggaga acggaggccc cgtcctcgag ataagggacc ttaccaagcg gttcggggcc
     5641 acgttagcct tgaacagcgt gagcctcacc ctgggacgta acgaagtgct cgcgctcctc
     5701 ggcgacaacg gggcggggaa gtctaccctg atcaaatgcc tcagcggcgt ccacgagccc
     5761 gatgaaggac agatactaat cgatggcaaa gaggtccaaa tcacctcacc gatgcaggcc
     5821 cgctctttag gcgtagagac ggtctaccag gatctcgccc tcttcgacaa cctcgacgtc
     5881 gtcggcaact tctacgcggg aagggagccg acgacgccgg gctggctcgg gaactggggt
     5941 tgggtcaagg acaaggatat ggcccgcgag gcggacgagc ttatgagccg cctggagata
     6001 aacctcccag gggccgacgt ggagacgggc ctgatgagcg gcggacagcg gcaggccgtc
     6061 gccagcgccc gtgcgatggc cttcgggcga aagatcgtga tcctcgacga gccgacctcg
     6121 gccctgggcg cccgcgaatc gcgcaacgtc ttgcgcctga ttcaggacgc cccgagccaa
     6181 ggggtctccg tgatcgtgat ctcccacaac ctcgaacacg tcatgcaggt ggccgacagg
     6241 gccgtagtcc tacgccaggg caacgtcgta ggagaggccg agccccacca ggagaaccac
     6301 gaacgcctgg tctcgatgat cgtcggcggc gctgacgagc cggcggacga cgaagagacg
     6361 gaagcttctg ccgaggagga catcgagaga gatgggtaga cgactggcgc tgccggcgcc
     6421 gctcttcttt cttatactcc tgctcgtcac ggggtgcggc gggcagctgg cgccctccta
     6481 tctgacggga gagatgaagg tcgggttcgt gcccaagacc ctggaccagg aattctgggt
     6541 gaccaccgag aagggtgcga gggccggcgg cagggaagcg gacgccaccg tgctcgtgca
     6601 ggctccaacc ggggagctgg ccgtagacga gcagatagcc atcgtcgaga atctcctgac
     6661 ccagaacata gacgcgctcg tcgtcgcgcc gtcggactcc atcctcatga agcccgtcct
     6721 ggagcgggcg agcgaggagg tgcccgtgat cctgctcgac acggatatac cgaagtggaa
     6781 gccgaaggac tcctacgtcg gtacggacaa cttcgcggcc ggactcgcgg cgggacgcta
     6841 tctggagcgg cagatgaacg gcaaaggtag catcggcctc ataacgggca tccccggctc
     6901 ggttcccggc gaccagcggg aggccggaat gcggaaagct atagaaaata cagagatcaa
     6961 ggtcgtcgga gaggtcgccg cagacttcga caggctcaag gcggtgggcg caatggagga
     7021 cctcttgcag acccaccccg atctggatgc cgtcttcgcg accaacgacc agatggccct
     7081 cggcgctata gaggcgctgc agacttacag gagcgatgcg atcctggtcg gtgtagacgg
     7141 ggctgtcgag gccacccagg cgatcatcgg cggcgacatg gacgccaccg tggcgcagaa
     7201 cccctacgcg atggggaagg tcggcgtcca gcaggccaca aaggccgcga acggcgagtc
     7261 tgtcgacccc aggatcaaca ccggcctcac gctcgtcacc aagaagaacg cgcccagcta
     7321 tctcaaagta agggaggagc aactcggctc cctgctcggt gtcgaggagt aggatgaagg
     7381 tcggactgct aggagcaggt cgtatcggcg ccctccacgc cggcgtgctg gcccgcgacc
     7441 ccactgttga cgagatactc atcggcgacg ccgacgcgca gcgtgccgag gaagtcgcac
     7501 aggaaatcgg aggggaagcg ggccggatcg aagccgtgct cgcctccgga cccgacgcag
     7561 tcgtcatcgc agcgtccacc ccggcgcacg cgcctttgat ccgggccgcc gtcgaggcgg
     7621 gcatcccggc tttctgcgag aagccgatag ccctgggata cgaggagacc gtcgagatag
     7681 cacacgaggt cgaggactcc ggcgcgacgc tccagatcgg cttccagagg cgcttcgacg
     7741 cgggatatgc cgaagcgaga cgcctcatag actccggcgc tctggggacc atctactctt
     7801 tgaggctcgc cacccacgac cccgacccgc cacccgaaga gtacatcccc gactcaggag
     7861 gtatcttccg cgaccttcac atccacgact tcgatatcct gcgctggctg accggcggtg
     7921 aggtagagga ggtgtacgcc acgggaagcg tccgcaactt cgatttcttc gccacgcacg
     7981 gcgacgtgga tacctctgcg gccctgatcc ggatgcaaga cggcgcggtc gccgtcttga
     8041 cagggggacg ccatgacccc ctcggctacg atgtgcgggc cgagatcttc ggctccagag
     8101 acagcgtcgc cgtcggcatg gacaggcgta cccctttgcg ctccgtcgag ccgggcatcc
     8161 cggcctcgga gaagggagcc tacccgaact tccaggaacg cttcctggat gcgtatacgg
     8221 cggagatcga acacttcctc tccctggtta ggggcgaggc agataaccca tgcacggcca
     8281 ccgacgcgct ggaggcgctc aggatcgcga tggcggcgga tctctcactg accgagcatc
     8341 gccccgtctc tccttcggag gtcggatgat gtcacgcgtt gcaggggcgc cgatctcctg
     8401 gggtgtttgc gaggtgcccg gatggggcta ccagatggat gccgagaggg tcctcgacga
     8461 ggccgccggc ctcggcctta cggcactcga agccggaccc gacggcttcc taccagggga
     8521 gccagacgtg atggcctctc tactctccca acacggactc gagctggtcg ggggcttcgt
     8581 gcccgtcgtg ctccatcgcc aggaggcgcg cgactctgag cttgcctcgg tagagaggca
     8641 ggccaagctc ttcgcagcgg ccggcgcggg cgtgctggtg atcgccgctg cgacggggga
     8701 ggagggctac gaggagtccg ctggcctaaa ggacggtgag tgggacgagc tgttcgccac
     8761 gctcgcgatg gtcgaggaga tcgggaccaa acacggcctc agcgtcgtcc tccacccaca
     8821 ttacggaacc gagatcgaaa gccccgaaca cgtacagcgt ttcctcgaag gttgcgacac
     8881 ggcactctgc ctcgatacgg gacattctat ggtcgggggc ggggatcccg ttgcgctggc
     8941 cgagtcggca gcgaccagga ttcgtcacgt ccacctgaag gacgtggatc aggacctcgc
     9001 cgaacgggtg gtctcgggca cgctgcctta cgaacaggcg gtcagggacg ggctctacat
     9061 gccgctggga gacggtgacg tggacgtccg gcgcgtgctg gaagtactgg acggggcagg
     9121 ctacgataga tggtacgtgc ttgaacagga cataatgctc tccgccgacc ctggctacgg
     9181 tcccgccgcc gaggtgggca ggagccttgc ctacctgaag ggagttctcg aggagtgata
     9241 cggaatccac atgatcgcgt ctccgccgta tacaagcggt gcgagcacac ctcgtacacc
     9301 ctatctctca cggtgaccat cccaccatcc gtcattcccg cttcttctct cgtcattccc
     9361 gcgaaagcgg gaatccactg cggcgagatc ttcgctcctc tgatgaatgg cgatctactg
     9421 gattacgcat gaaccctgaa gtgattacga tgggaagggt gagcgtggac ctttatcccg
     9481 agcagatcgg ggtcacgctc gccgaggtcg agaccttcgc caagtccctc ggcggcagcg
     9541 cgacgaacgt ggccgtcgcc gccgcccgcc tcggccaccc ctccgccctc ataaccaagg
     9601 ccggcgacga cggcttcggc cagtacgtcc gaaccgcgct ggaagggttc ggtgtggaca
     9661 ccacctgggt cagcacggac cccgagctgc ggacgcccct ggcattctgt gagatccacc
     9721 ctcctgacga cttcccgatc ctgttctacc gcgagccgaa ggcccccgac atgaacctga
     9781 cggtcgaaga cctcgacttc gacgcgatct cggccgttcc cttgttctgg atcaccggta
     9841 ccggcctctc cgacgagccg agccgtacgg cgaccctcgc cgccctcgag cacaggccca
     9901 cgggcatcac ggtcttcgac ctcgactaca gacccacgct ctgggcctcg aaagaggagg
     9961 cggcccactg ctacagggag gcgctgcggc acgccacggt ggccgtcggc aaccagagcg
    10021 aggtcgaggt cgccgtgggc acgggagacc ctttcgaagc ctcggaggcg ctactggacc
    10081 tcggcataga gctcgcgatc gtcaaacggg gaccggaagg cgtgctcgcc cgcacgaaag
    10141 acggtgtggc cgaggccccg ccgatagaga tagagatcgt caacggcctg ggcgcgggcg
    10201 acgccttcgg aggagcgctc tgccacgcgc ttctctcggg gtgggacgcc gagaggacca
    10261 tccgctacgc gaacgccgca ggggccatcg tcgcctctcg cctggcgtgc gccgacgaca
    10321 tgccgacccg cgacgaagtg gaacaactcc tggagaagtc ctcaaatgcc tgagagccat
    10381 ttcccctttc tctctggtca ttcgcccctt cattctcgtc attccctccc ttcctctagc
    10441 tattcccgcc gctcaacttg ccatgcccct ccacttgtca ttcccgcgaa agcgggaatc
    10501 caccgcggca agagttccgt accttccaca aggatcggtc tgccggatta tgctctacgg
    10561 atcttcgaac ggaggtgacc acttgaccac ggtacgacta acgatggccc aggcgctggt
    10621 acgcttcctg accgagcagt acgtagagcg agacgggatc gaacaccgct tcttcgccgg
    10681 atgcttcggt atcttcggcc acggtaacgt cgccggtgtc ggccaggcgc tccagcagta
    10741 cgcgggtggc ctcccttact accaggcccg caacgagcag gcgatggtac acaccgcctc
    10801 ggcctacgcc cgccagaaag accgcctcca ggccttcgcc tgcacgacct ccatcgggcc
    10861 aggcgccacc aacatggtta cgggagcggc cctagccacg at
//
LOCUS       DATMVF010000091         8520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_287793, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000091 DATMVF010000000
VERSION     DATMVF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287793"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VN178_04200"
     CDS             <1..739
                     /locus_tag="VN178_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWS80204.1"
                     /translation="FVIPSYVLQNSIDAEQMLELAASHSTRELQELVAATEPPREKIT
                     AAYENLVGGRLTATYKLVGNKKVAVRSSAVAEDSEAASYAGQQETYLFVEGAEEVCRR
                     VVDCWASFFSERALFYRSHKGSLEDLRIAVVVQMMVDPEKSGVIFTVDPVRRRRDRMV
                     VEAVRGIGEQVVSGEVTPDHYTLDRRGNVKREKVVDDRVLTDGELQRLGDLGRQLEER
                     HGVAQDIEWAMVGEEIYLLQSRPVTTM"
     gene            783..1616
                     /locus_tag="VN178_04205"
     CDS             783..1616
                     /locus_tag="VN178_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWS80205.1"
                     /translation="MIAPEWHEPRSLDEALSLRAEHGEEATVVAGGSFLGIVMNQRLF
                     APTMLVALRSVPDLDYIGADGGPLRLGAMTTHRAVERSPVVKEGWPSLAFTFSVVASP
                     RVRNQATVGGVVADADYASDPPSMLAALEARVVARSVRGEREIPMNVFVLDHYETALE
                     EDELVTELRIPRSEGRSVYRKFSSRSSEDRPCVSVAAAQEDGRLRVVVGAVAGRPQYF
                     PEICDLAKGEHLSGELATEIGRRYAEEIEPLSDSRGSARYRSRVIAVEVRRALEDLNH
                     D"
     gene            1609..3969
                     /locus_tag="VN178_04210"
     CDS             1609..3969
                     /locus_tag="VN178_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWS80206.1"
                     /translation="MTDPRVTGAVRYSQDVAMEGMLHARLLRSPVAHARVVGVDASAV
                     PEGIIVLLPEDVQDLGGYGPQVKDQEILPQERVRYAGDVVAAVAAEIADEAEEAVGLI
                     NVDYEELPAVFDEVEAASEGAILVHETTNISRNDAAYFGIRPQQETNICHLFRLRHGD
                     VEAGFGEADAVVEETYHTAGATHVPMEPHAAVARWEWDRLEVVSGTQTPFNMRTDLAG
                     LFSIDEERVRIISPPMGGSFGAKTFVRTEAIAACLARKAKRPVKIVLERDEMWMTLNR
                     HPATVRVKLGARSDGTLVASETECWANTGAYADCGPGVAQKMGFAAPGPYRIANVRVD
                     SRAVYTNLPPNGAFRGYGQMQSTWARERTMDLLADKLGMDPLELRMKNLLKDGDKYCT
                     GETMHDVHFERLLQDAADAVAWTESRKNKGLCVMLKGMQTPSRAAIAVEIEDGGTYTV
                     RCATTEMGQGAKMAMRMMAAELLDVGVEKVSCPDPDTDVVPYDTRTTSSRSTHMMGRA
                     LKVAVADLKENGERGYGEVVDEGGLDPDTGQGVASSHWHQGAAAAEVEVDEETGRFGV
                     MKLHAPIYAGRVINRPAAELQNEGSMIMGLGTALFESNEFAEGQITNANLSDYNVPAM
                     DDMPATLSHELVEREGGEVQGLGETALPPVPPAIGNALYSRGIHVTELPISAESVLDA
                     VDTRDGRGDAWAKAGCEPVSTDGRNDSPGERVFASGTAPEPTPTPVNGSDGLSDQGRL
                     SSVRFLFASPSALLLLGTLGIGLLAIALYRLLHRRGAAEKKALLRS"
     gene            3986..4465
                     /locus_tag="VN178_04215"
     CDS             3986..4465
                     /locus_tag="VN178_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWS80207.1"
                     /translation="MKVRLNVNGQPVEVEARADEMLLDVLRGELDLTSVRYTCGIGVC
                     GACTALVDGEPISTCILLAPLAEGKEITTVEGLGGNHSVQRAFVEAEAFQCGYCTPGM
                     ILTAKSLLEENPEPTEERIRAYMGGNLCRCGCYPKIEDAVNLAASMSKAATPIKGGT"
     gene            4462..5298
                     /locus_tag="VN178_04220"
     CDS             4462..5298
                     /locus_tag="VN178_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWS80208.1"
                     /translation="MRLVTYDAGQGPRVGILQGDAVLDAGFDGDMIAFIEAGAPAGSQ
                     TPVDNARLLSPLRPRSMRDFLAFEGHLKNAFRRLGRPIPDEWYEVPAFYRAMPDTVIG
                     QGETVPYPPYTEKLDHELELAAIIGRRGMDVPREEADSYVFGYTIWNDLSARDTQARE
                     LPVGMGPSKAKDWDGSNVLGPCIVTADEVDAGDLTMSVRVNGETWGDDNSSNMHYKFA
                     DMIAYVSRAQTIYPGEVFGSGTAEGGAGIETDRYLSEGDVVELEIEGIGVLRNTIGKK
                     GA"
     gene            5303..5593
                     /locus_tag="VN178_04225"
     CDS             5303..5593
                     /locus_tag="VN178_04225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWS80209.1"
                     /translation="MAFVVVAKWTTREGEADKVEEAIRKLTPLSREEPGNLFYQAHRD
                     PENPNLFFLYEQYVDEDGYKAHGDSEHFQEYGFGTAIPLLESREREFYVTLD"
     gene            5611..6300
                     /locus_tag="VN178_04230"
     CDS             5611..6300
                     /locus_tag="VN178_04230"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWS80210.1"
                     /translation="MKILDLSPAVPHGFKGPPSTDLGVQMNVRTKNGTGYWQSTQLDL
                     MSLHTGTHVESALHTIKGGEAIDEVALDRMIGEAVVLDLTPTEPMQIIDVADLEKANE
                     SLEAAGESIRSGDILLLRTDWAQRHIGTQKYFRESPGLTAEAAGWIVERRPKSVGCDF
                     FEEPAARQPGWTADEFVVHGAILGAGIPLIEGLVNLRDLPLRVRFFAPFYKFSRVESA
                     PARAFALIETE"
     gene            6329..7048
                     /locus_tag="VN178_04235"
     CDS             6329..7048
                     /locus_tag="VN178_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWS80211.1"
                     /translation="MRIHWVRHGKIASHRGNVPLTDEGVAQARERGRLLAEDLSPGEI
                     VHFMHAPTLRTQQTVEEIRASMAESLGPDGDADLLEVSEQWAIRNPDLFLAGQRVEMV
                     STAETLADQLCAPSVDPQTLSEHPFFREFWNSPDRIGYWLRHLDPPGEDALAVARRQM
                     TFAMSLPDKQENRPVRYVLSTHSPVQRAILLCYTGEDAGEPGYLEPIDLTLSENGSQE
                     LRFRGLRTLLSGTKVVKGVPR"
     gene            complement(7029..7697)
                     /locus_tag="VN178_04240"
     CDS             complement(7029..7697)
                     /locus_tag="VN178_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80212.1"
                     /translation="MSRGRRRKAQPTDEWQELLPLFEWPEQQAYEELRPLVLFGSSVA
                     ERATETGVPERTMYRRIERFERDGMVSLFAADPAAARARRRGVEPAIRRMIVELKAGH
                     PRLNASEISNIVYVRTGRRLGKHTPARVLYEEVVPLKLSRLFEPDHETEDEREARKAV
                     VALHLDGWSVKSIASYLRVSRTTGATLRSYLFGYDAPFRTQADSMSIFVIVAPHVPPT
                     VVRL"
     gene            complement(7833..>8520)
                     /locus_tag="VN178_04245"
     CDS             complement(7833..>8520)
                     /locus_tag="VN178_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HWS80213.1"
                     /translation="TPGTRRPGNRAAGKEKPVIPDTQTLVLFLGASLAITLAPGADML
                     YVVSRGVAQGRTAALVSAAAVNSGSLIHLLFAVLGLSLLLQQSALAFYAVKYAGAAYL
                     VYLGVRILLDRNGLRPSEGAVTSRRLAGVFVQGVVTNLLNPKAYLFFLSFLPQFVDPS
                     AGGVAARMLLLGLVFVGIGVVCDGLIAWFSGGLGESLARRPRLSAAVRWLAGGILVAL
                     GLRLAVPERH"
BASE COUNT         1624 a         2621 c         2868 g         1407 t
ORIGIN      
        1 cttcgtcatc ccctcgtatg ttcttcagaa ttccatcgac gccgagcaga tgctggagct
       61 cgcggcctcc cactccacaa gagagctcca ggagctcgtc gcggctaccg aacctccgag
      121 ggagaagata acggccgcct acgagaacct cgtgggtggg cgactcacgg ctacgtacaa
      181 gcttgtgggc aacaagaagg tggccgttcg ttcctcagcg gtcgcggagg actctgaggc
      241 tgcgagctac gctggtcagc aggagaccta cctcttcgtc gaaggtgccg aggaagtctg
      301 caggcgggtc gtggactgct gggcgtcttt cttctccgag cgcgcgctct tctatcgttc
      361 gcataagggc tccctcgaag atctgcggat agccgtagtg gtccaaatga tggtcgaccc
      421 cgagaagtcc ggcgtgatct ttaccgtaga ccctgtcagg cgtcgccgcg accggatggt
      481 tgtagaggcc gtcaggggca tcggggagca ggtcgtctcc ggcgaggtga ccccggacca
      541 ctacaccctg gaccgtaggg gaaatgtcaa gcgcgagaag gttgtagatg accgcgtcct
      601 taccgacggg gagctccaga gactcggcga cctaggcaga caactagagg aacgtcacgg
      661 cgtcgcacag gacatcgagt gggcaatggt tggcgaggag atctacctcc tccagtctcg
      721 gcccgtcacg acgatgtaga cgcactgttt gacgcggttc tgaaagggtt tttgagatgg
      781 caatgatcgc accggaatgg cacgagccac gctcgctgga cgaggctctc tccctgcgtg
      841 ccgaacacgg ggaggaggcg accgtcgtag ccggcggctc ctttctcgga atcgtcatga
      901 accagaggct cttcgccccc acaatgctcg ttgcccttcg cagcgtcccg gacctcgact
      961 acataggggc cgacggcgga ccgttacggc ttggggcgat gaccacccac cgggctgtag
     1021 agcgctcgcc ggtagtgaag gagggctggc cgtcgcttgc cttcaccttc tccgtggtcg
     1081 ccagcccgcg tgtcaggaac caggcgaccg tcggcggggt cgtcgccgac gcggactacg
     1141 cctccgatcc tccctcgatg ctcgcggctc ttgaggcgcg ggtcgttgcg cggagcgtga
     1201 ggggagagcg cgagataccg atgaacgtgt tcgtcctaga ccactacgag acagcactcg
     1261 aagaggacga gctcgtcacg gagctccgga taccccgcag cgagggacgc tccgtctacc
     1321 ggaagttcag ctcgcgctca agcgaggatc ggccctgcgt ctccgttgcg gcggcccaag
     1381 aggacggcag gctacgggtc gtggtcggcg cggtcgctgg acggccccag tacttcccgg
     1441 agatttgcga cctggcgaag ggagagcacc tgagcggcga gctcgccacc gaaattggac
     1501 gccgctacgc cgaggagatt gagccgctct ccgattcacg cggctcggct cgttaccgta
     1561 gccgtgtcat agccgtggag gtcaggcgcg cccttgagga tctgaaccat gactgatcct
     1621 cgcgttaccg gagccgtccg ctactcgcaa gacgtggcaa tggaggggat gcttcacgcc
     1681 cgcctcctgc gatcgcccgt ggcccacgcc cgcgttgtgg gggtggacgc ctcggcggtc
     1741 ccagagggca tcatcgtcct cctcccggag gacgtccagg atctcggcgg ctacgggccg
     1801 caggtaaagg accaagaaat cctccctcaa gagcgagttc gctacgccgg ggacgtagtc
     1861 gcagccgtcg cggccgagat tgcggacgag gccgaggagg cggtggggct cataaacgtc
     1921 gactacgagg agcttcccgc cgtcttcgat gaggtggagg ccgcctcaga gggggcgata
     1981 ctagtccacg agacgacaaa catctctagg aacgacgccg cgtacttcgg cattcgaccg
     2041 cagcaggaga cgaatatttg ccacctcttc cggctgcgac acggcgacgt cgaggccggg
     2101 tttggggagg ccgacgccgt ggtcgaggag acctaccaca ccgccggggc tacgcacgtc
     2161 ccgatggagc cccatgctgc cgtcgctcgc tgggaatggg accggttgga ggtcgtgtcc
     2221 ggcacacaga ccccgttcaa catgcgcacg gaccttgccg ggctcttcag catagacgag
     2281 gagagggtca ggataatctc accccctatg gggggctcgt tcggggcgaa gacctttgtc
     2341 aggacggagg cgatagccgc gtgcctcgct cggaaggcga agaggccggt caagatcgta
     2401 ctcgagcgcg atgagatgtg gatgacgctt aaccgccacc cggccacggt ccgggtgaag
     2461 ctcggtgctc gaagcgacgg aacgctcgtc gctagcgaga ccgagtgctg ggccaacacc
     2521 ggggcctacg ccgactgcgg tcccggcgtc gcccagaaga tgggcttcgc cgctcctggg
     2581 ccgtaccgca tcgccaacgt tcgggtcgac tcgcgcgccg tctacaccaa ccttccgccc
     2641 aacggcgcct tccgcggcta cggccagatg cagtccacct gggcgaggga gcgcaccatg
     2701 gacctcctcg ccgacaagct cgggatggac ccactggagc tgaggatgaa gaaccttctc
     2761 aaagacgggg acaaatactg caccggcgag actatgcacg acgtccactt cgaaaggctc
     2821 ctgcaggacg ccgccgacgc tgtggcctgg accgagagcc ggaagaacaa gggtctgtgc
     2881 gtcatgctca agggcatgca gactcccagc cgagcggcga tcgcggtgga gattgaagac
     2941 ggcggaacct acaccgtccg ttgcgcgacg accgagatgg gccagggcgc aaagatggcg
     3001 atgcgcatga tggccgccga gctgctagat gtgggcgtgg agaaggtcag ttgccccgac
     3061 ccggacaccg acgtcgtccc gtacgacact cgtacgacct ccagccgctc gacgcatatg
     3121 atgggccggg cgctcaaggt ggccgtcgcc gacctgaagg agaacggcga gcgcggctac
     3181 ggcgaggtcg tagacgaagg cgggctcgat cccgataccg gtcagggcgt tgcttcgtcg
     3241 cactggcacc agggggcggc ggccgctgag gtcgaggttg acgaagagac aggcaggttt
     3301 ggggtgatga agcttcacgc gccgatctac gcgggccggg tgataaatcg gcccgcggcc
     3361 gaactccaga acgaggggtc catgatcatg ggcctaggaa ccgccctctt cgagagcaac
     3421 gagttcgctg aaggccagat cacaaacgcc aacctctccg actacaacgt cccggcgatg
     3481 gatgacatgc ctgcaaccct ctcgcacgag ctggtggagc gcgagggcgg agaggtgcaa
     3541 ggcctcggcg agaccgccct tccgccggtc ccgcccgcga tcggcaacgc tctctactcc
     3601 cgcggcatac acgtgacgga gctgccgatc tccgcggaga gcgtcctcga cgccgtggac
     3661 acccgagacg gcagaggcga tgcgtgggcc aaagccggct gcgagccagt ctccacagac
     3721 ggccggaatg acagtccggg agagcgcgtt ttcgcatccg ggactgcccc ggagccgacg
     3781 ccgacgccgg tgaacggttc agacggcctg tcggatcagg gacgtctgag tagcgtgaga
     3841 ttcttgttcg cttcgccgtc cgctctgctc ctcctcggga cgctcggtat agggctccta
     3901 gcgatcgcgc tctaccggct gctccaccgc agaggcgcgg cggagaagaa ggctttgctc
     3961 aggtcatagg tactgaggag gtaggatgaa ggttcgactc aacgtaaacg gacagcccgt
     4021 agaggtcgaa gctagggcgg acgagatgct tctcgacgtt ctcagaggcg agctcgacct
     4081 aacgagcgtc cgttacacct gcggcattgg ggtttgcgga gcgtgtacgg cgctggtaga
     4141 cggcgagccg atctcgacgt gcatcctgct cgccccgctc gcggagggga aggagatcac
     4201 gaccgtcgag ggactcggag ggaatcattc cgtccagagg gcgtttgtcg aggcggaggc
     4261 ctttcagtgt ggctactgta ctcccggcat gatcctcaca gccaagagcc tactagaaga
     4321 gaacccggaa ccgacggagg agagaattag ggcctacatg ggtggcaacc tgtgtcgttg
     4381 cggctgctac cccaagatcg aagacgctgt gaacctggcg gcaagcatga gcaaggctgc
     4441 cacgccgatc aagggaggga catgaggctg gttacctacg atgccgggca ggggcccagg
     4501 gtagggattc tccagggcga cgccgtcctg gatgccggtt tcgacggcga catgatcgcc
     4561 tttatcgagg ccggtgcccc ggccggttcg cagaccccgg tagataacgc ccgactgctc
     4621 tctcccttga ggccgcgctc tatgagggac ttcctcgcct ttgaggggca cctcaagaac
     4681 gccttccgcc gtctggggcg gccgataccg gacgagtggt acgaggttcc cgcgttctac
     4741 cgggcgatgc ctgacaccgt gatcgggcag ggggagaccg ttccttaccc accttacaca
     4801 gagaagttgg accacgagct ggagctcgcc gccataatcg ggcgtagggg catggacgtc
     4861 cctcgcgagg aggcggatag ctacgtcttc ggctacacga tctggaacga cctctcagcc
     4921 cgcgataccc aggctcgcga gttgccggtg gggatgggcc cgtcgaaggc gaaggattgg
     4981 gacggctcca acgtcttggg cccctgcata gtcacggccg acgaggtgga tgcgggtgac
     5041 ctgacgatga gcgtccgcgt caacggcgag acgtggggtg acgataactc atccaacatg
     5101 cactacaagt tcgcggatat gatcgcctac gtttcccgag cgcagacgat ctaccccggt
     5161 gaggttttcg gctcagggac tgccgaaggg ggtgcaggca tcgagacgga ccgctacctg
     5221 agcgagggag acgtcgtcga gctcgagata gagggcatcg gcgttctgag gaacacgatc
     5281 ggaaagaaag gagcatagag atatggcatt cgttgtcgtg gcgaagtgga cgactagaga
     5341 gggcgaggcg gataaagtcg aagaggcaat aaggaagctc actccgctct cacgggaaga
     5401 gccgggcaac ctgttttatc aggctcaccg cgatccggag aacccgaacc tgttcttcct
     5461 ctacgagcaa tacgtcgatg aggacggcta caaggcgcac ggcgactccg agcacttcca
     5521 ggagtatggc ttcggcacgg cgataccgct cctcgagagc cgggagcgcg agttctacgt
     5581 gacgctcgac tagcaggaag gagcgtctcc atgaagatct tagacctcag tccggctgtc
     5641 ccgcatggct tcaagggacc accctcgacg gatctcgggg tgcagatgaa cgtcaggacg
     5701 aagaacggaa caggctactg gcaatctacg cagctcgacc tgatgagcct gcacaccgga
     5761 acgcacgtgg agtccgccct ccacacgatc aagggcggtg aggccataga cgaggtcgcg
     5821 ctggatcgga tgatcggcga ggcggtggtc ctcgacctca cgcccacgga gcctatgcag
     5881 atcatagacg tggccgacct cgagaaggcg aatgagagcc tagaagccgc gggcgagtcg
     5941 atccggtccg gcgacatcct gctccttagg accgactggg cccagaggca catcggaact
     6001 cagaaatatt tcagggagtc gcccggcctg acagcggagg ctgcggggtg gatcgtcgag
     6061 aggaggccga aatccgtcgg ctgtgacttc ttcgaagagc ccgcggcccg gcagccaggc
     6121 tggacggcgg acgagttcgt cgtgcatggc gcgatcctgg gcgcgggcat cccgctgatt
     6181 gagggtctcg tgaacctgag agatctaccg ctgcgggttc ggttcttcgc tcccttctac
     6241 aagttctcga gggtagagag tgcccctgcc cgcgcgttcg ccctgatcga aaccgagtaa
     6301 ggtgagggga gtagctctgt tatccaacgt gaggatccat tgggtacggc acggtaagat
     6361 cgcttcgcac aggggaaacg tccccttgac ggatgaaggg gtagcccagg ctcgagagag
     6421 agggcggttg ctcgccgagg acctctcacc cggggagatt gtccacttca tgcacgctcc
     6481 taccctccga acccagcaga cggtcgagga gatccgggcg agcatggccg agtccttagg
     6541 gcccgatggc gatgcggatc tgctcgaagt cagcgagcag tgggccatcc gtaacccgga
     6601 tctcttcctc gcgggccagc gggtagagat ggtgagcaca gccgagaccc tcgcggacca
     6661 gctctgcgcg ccgtcggtgg atccccagac actctcggag catccctttt tccgcgagtt
     6721 ctggaattcg ccagaccgta taggctattg gctccgccat cttgacccac ccggcgagga
     6781 cgcgttggcg gtcgcccggc gccagatgac cttcgccatg agcttgccgg acaagcagga
     6841 gaacagaccg gttcgttacg tactctcaac ccattctccc gtgcagcggg cgatcttact
     6901 ttgttacacg ggagaggacg cgggcgagcc ggggtacctg gagcccatag acctaactct
     6961 gtcagagaac gggtcgcagg agcttcggtt tcgaggcctt cgaacgttac tttccggtac
     7021 taaggtggtc aaaggcgtac cacggtaggt ggtacgtggg gggcgactat cacgaagatg
     7081 gacatcgagt ccgcctgtgt cctgaaagga gcatcgtatc cgaaaaggta ggaccgaagc
     7141 gtggccccgg tggtcctgct gactcggagg taggaggcta tggacttcac gctccagccg
     7201 tccaggtgca aagccaccac cgccttgcgc gcctccctct cgtcctcggt ctcgtggtcg
     7261 ggctcgaaga ggcgcgagag cttcaggggg accacctcct catagaggac cctcgccggg
     7321 gtgtgcttgc cgagcctgcg gcccgtcctc acgtatacga tgttcgagat ctcgctggcg
     7381 ttgagccttg ggtgcccggc cttcagctcg acgatcatac gccggatggc gggctccacg
     7441 ccccgacgcc tggccctcgc ggcggcgggg tcggcggcga agaggctcac catcccgtcc
     7501 ctctcgaagc gctcgatcct gcggtacatc gtgcgctcgg ggacgcctgt ctccgttgcc
     7561 cgctcggcca ccgagctgcc gaacaggacc agcggcctga gctcctcgta ggcttgctgt
     7621 tccggccact cgaagagcgg cagcagttcc tgccattcgt cggtcggttg cgccttccgg
     7681 cgtctgccgc ggctcatgct ccccctccac cgcgcatcgt gggacgggaa gaatgccaca
     7741 acgaggcatc cgcagccagt tcggactgtg caccggccga acgcggaggc catggcgcct
     7801 tgcgcccacc taatcgcgcg agggaggagg agctagtgtc gctcggggac ggcgagcctg
     7861 aggccgaggg cgaccaggat gcctcccgcc agccaccgta ccgcggccga caggcgcggc
     7921 cgtctcgcca gcgactcacc cagcccgccc gagaaccagg cgatgaggcc gtcgcagacg
     7981 accccgatcc cgacgaacac tagccccaag agcagcatcc gggccgcgac gccccccgcc
     8041 gaggggtcaa cgaactgcgg caggaaggag aggaagaaga ggtacgcctt gggattgagg
     8101 aggttggtga ccacgccctg gacgaagacg cccgcgagcc ttctgctggt gacggcccct
     8161 tctgatgggc ggaggccgtt gcggtccagc aggatcctca cccccaggta gacgaggtag
     8221 gccgcacccg cgtacttgac ggcgtagaag gccagagcgg attgctgcag cagcaaggac
     8281 aggccgagca cggcgaagag gaggtggatg agcgaccccg agttgacggc cgccgccgag
     8341 accaacgcgg cggtgcggcc ctgcgccacg ccgcgggaga ccacgtagag catgtcggcg
     8401 ccgggggcga gggtgatggc cagcgacgcg ccgaggaaca ggaccagggt ctgggtgtcg
     8461 gggatcacgg gtttctcctt tcccgccgcg cggtttccgg ggcgtcgcgt cccgggagta
//
LOCUS       DATMVF010000092         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_474439, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000092 DATMVF010000000
VERSION     DATMVF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474439"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            563..1648
                     /locus_tag="VN178_04250"
     CDS             563..1648
                     /locus_tag="VN178_04250"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWS80214.1"
                     /translation="MCFGATNSYTVGVEEEFQLIDPSTRELTPAIEGVIGASLENTER
                     IAPELFQDCVEMRTPVFSSVAELARDLPTLRLDVAKAAGRAGVRMAASGLHPISDPFS
                     QRFTRGERYERMEEELGALARLQIIYALHVHVAVVDEESAIRATNALLRHVPLFVALS
                     ANSPFWNGMDTGFASARVKIRDASPRSGLPPTFHSWEEFENYTDALVAMGNIPDFSWF
                     WWDVRPHPRHGTVELRVPDAQAEIGNVVALAALTQCLVATAEDYAPEDARFVSENKWR
                     AIRRGLDARLYDFETKEEKGARETARALVSRLRPVSQDLGCETELEGVLEILHRGTGA
                     ELQRAAYERRGSLEDVVDYLLETTASA"
     gene            1775..2512
                     /locus_tag="VN178_04255"
     CDS             1775..2512
                     /locus_tag="VN178_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80215.1"
                     /translation="MLHRDVAEGVHRIGEHFLNWYLVEEGGRLTVVDAGLPASWRSLL
                     GALEGIGRAPSDIEALVLTHAHFDHIGFAERARAELSIPVWVHESDVPLTKRPWLYMS
                     ERSPFAYLGRKTLPIVASFVRAGAPRVGPIREVRRFGDEGTLDLPGSPRVVFTPGHTL
                     GHVALHLPERDAVITGDALVTHDIYTDIRGPRIVARGATADSERALASLERLADTGAG
                     TLLPGHGEPWTGGAEQAVKEARQAGVL"
BASE COUNT          512 a          890 c          865 g          510 t
ORIGIN      
        1 aggcttgctc tatcggtgat cccgagcggc gtagctctag gtactctcct acgaagtcgg
       61 cggaatcggg ggtgccagcg agcatctgta cggcttgctc gtccgttgag ggtgcttgta
      121 ccctctcgga ggtgcggcac gtggggttgt accaatacat cttcttgtgt cctccttccc
      181 tgaaaggacg atgcctctga tgtaccctct gctgcgagcg ataaaacgcc taccgaggca
      241 attttgtctt aacggggttc cccgaagctc ggcactaaaa gagggctagg gccggggccc
      301 tagccctcgc taccttcccc aacatgagag cctacatatt tcgtcctcct actgagttat
      361 ctaattcatc tattgatcgt ccctgttcga gcagtcgacg actgtatcgc taactgtatc
      421 gctatcgatg cgctgcccaa agcccacatc tgactccgga tctccagatc gagctgcacg
      481 agggtttcgt ctctcggatt ctagctaccg ccttccttca ggtgtgagct cgtgttgccc
      541 tgggtaaggg ggcggaatgg agatgtgctt tggggcaacc aactcgtaca ccgtgggtgt
      601 ggaggaagag ttccagctaa tcgacccctc cactcgggag ctgactccgg caatagaagg
      661 tgtgatcgga gcctctctag agaacaccga gcggatagcc ccagagctct ttcaggactg
      721 cgtggagatg cgcacccctg tgttctcgtc cgtcgcggag ctggcccgcg atcttcccac
      781 tctccggctc gacgtcgcga aggcggcggg cagagcaggg gtaaggatgg cagcatcggg
      841 gctgcacccg atctccgacc ccttctcaca gaggttcacc cgcggggagc gctacgagcg
      901 gatggaggag gaactgggcg ccctggcacg actccaaatc atctacgcgc tgcacgtgca
      961 cgtagccgtc gtggatgagg aaagtgcgat tcgcgccacg aatgctctcc ttcgacacgt
     1021 tccgctcttc gtggcgctct cagcgaactc tccgttctgg aacggcatgg acacgggctt
     1081 cgcctccgcc cgcgtcaaga tccgggatgc ctctccccgc tcggggctcc ctccgacgtt
     1141 ccatagctgg gaggagtttg agaactacac ggacgccctc gtcgccatgg gcaatatccc
     1201 ggacttctcc tggttctggt gggacgtgcg gcctcacccg cgacacggca cggtcgagct
     1261 tcgagtcccc gacgcccagg ccgagatcgg taacgtcgtc gcgctcgccg ccctcaccca
     1321 gtgcctggtg gcgaccgccg aagactacgc cccagaagac gcccgcttcg tctcggagaa
     1381 caagtggcgt gcgatccgcc gcggactcga cgctcgtctc tacgacttcg agacgaagga
     1441 ggagaaggga gcaagggaga ccgctcgcgc tctcgtcagc cggctgcgtc ccgtctcgca
     1501 ggacctcggg tgcgagaccg agctagaggg cgtcctggag atacttcaca ggggcactgg
     1561 cgccgagctg cagagagccg cctacgagag gcgcggctcc ctcgaagacg tggtcgatta
     1621 cctgctcgag accacggcgt cggcctgaga tgataccgaa tggctggccg gcgcaggatt
     1681 ctctatgggc atcccttcgt tggacacgtc cgataacctc aatctatagg cacgacgaat
     1741 accccagaag cgcccacggc gggaggagtt atcgatgctg caccgcgacg tagccgaggg
     1801 cgttcaccgg atcggcgaac acttcctcaa ctggtacctc gtggaggagg gaggacggct
     1861 caccgtggtc gacgccggcc taccggcctc ctggcgttcc ttgcttgggg cactggaagg
     1921 gattggccgc gcgcccagcg atatcgaggc gctcgtgctc acgcacgccc acttcgacca
     1981 tataggcttc gccgagcggg cgagggcaga gctgagcatc ccggtatggg tccacgagag
     2041 cgacgtaccg ctgacgaagc ggccgtggtt gtacatgagc gaacgcagcc cgttcgcgta
     2101 ccttggtcgc aagaccctgc cgatcgtggc ctccttcgtc cgggccgggg cgccgcgcgt
     2161 gggcccaatc cgcgaagtgc gccggttcgg agacgaaggc accctcgact tgcccggctc
     2221 ccctcgcgtc gtgttcacgc cgggtcacac cctgggccac gtcgcgctcc acctgccaga
     2281 gcgcgacgcg gtcatcacgg gagacgcgct ggtcacgcac gacatctaca ctgacattcg
     2341 gggtcccaga atcgtcgcgc gtggggcgac ggccgacagc gagcgggcgc tggcctccct
     2401 cgagcggctg gcggacacgg gagccgggac gctcctgccc ggccacggtg agccttggac
     2461 aggtggcgcc gagcaggctg ttaaagaagc ccgccaggcg ggcgtcctct agccttcgca
     2521 ggatcgtcgc aggtcttgcg gatgtgacca aaaccgccac tgtaggagcg ggagcataat
     2581 ctgttaacgc agagacgaaa agggtacaga agccgaaaaa gaaaggaatc atctccatgc
     2641 tcgccatgaa ctacaggggc ccccaaaggg tccgcgtcga ccacaaaccc atgcccgaga
     2701 tcttgcaccc tgaggacgcc atcgtccgcg tcacgcgctc ttgcatctgc gggtccgatc
     2761 tccatctgta caacggg
//
LOCUS       DATMVF010000093        16844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_422821, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000093 DATMVF010000000
VERSION     DATMVF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16844
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422821"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VN178_04260"
     CDS             complement(<1..388)
                     /locus_tag="VN178_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HWS80216.1"
                     /translation="MGKNVKALSLVPREQRFYDLFEQQAAILVSAGVLLKEALDDMGN
                     LPARRREIKDLEHQGDKITYETMRTLNETFVTPFDHEDISALAAGLDDILDYIEEVAD
                     TVNLYGITSIPRPAGELADLLAQAVVQ"
     gene            complement(671..2191)
                     /gene="guaA"
                     /locus_tag="VN178_04265"
     CDS             complement(671..2191)
                     /gene="guaA"
                     /locus_tag="VN178_04265"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWS80217.1"
                     /translation="MILVLDFGGQYAQLIARRVRECRVYSEMIPYDTPVDEIRAKDPE
                     GIILSGGPNSVYEEGAPRVGPDLFDLGVPMLGICYGMQLMALVEGGEVGAVQIREYGR
                     SDLVVKSHEGLFAGTPDEQKAWTSHGDAVLSLPEGFEITAETPSVPIIAFEDGAQGRF
                     GVQFHPEVRHTEYGMEILKNFLFEACGCRPEWTPVNIITDAVERIRGQVGEARAICGL
                     SGGVDSATAAMLVHRAIGDNLTCVFVDHGLLRHNEAEQVVEAFAQNSDVPLVHVDAAG
                     RFLDKLADVTDPEAKRKIIGEEFIRTFEEEAGKLEDATFLVQGTLYSDVIESGTRDAA
                     RIKSHHNVGGLPEVMDLDLVEPLRNLFKDEVRVVAAELGMPERLVWRQPFPGPGLAIR
                     VIGDVTAERLEILRKADAVLQDEIRNAGLYRELWQSFAVLPAIHSVGVMGDARTYAYP
                     IVIRAVTSDDAMTADWARLPYDLLERTSNRIINEVPGVNRVALDITSKPPGTIEWE"
     gene            complement(2217..3377)
                     /locus_tag="VN178_04270"
     CDS             complement(2217..3377)
                     /locus_tag="VN178_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HWS80218.1"
                     /translation="MDIEIGRGKTARRAYGLDEIAIVPSRRTRDPEDVDISWALGNLN
                     LDLPCLASALDAVVDPTTAGIIGSLGGLAVLNLEGLQTRYEDPGPVFEEIASLPEHKA
                     TRIMQDIYAEPIKEELIFRRVQEIKDKGVIAAASLTPQRVERYHRAAIEAGLDVLVVQ
                     GTVVSAEHVSRSVEPLNLMEFVPSLNVPVIVGGCASYSTALHLMRTGAVGVLVGVGPG
                     RICTTRGVIGVGVPQATAVADAAAARMRHYLETGEYVNVIADGGMRTGGEIAKAITCG
                     ADAVMLGSAFARAEEAPGRGYSWGMATFHPTLPRGTRISTRTTGSLEEILVGPAREND
                     GTLNLMGALRTSMATTGYQNIKEFQKAEVMYAPSLATEGKTEQRSQGVGMGR"
     gene            complement(4020..5642)
                     /gene="groL"
                     /locus_tag="VN178_04275"
     CDS             complement(4020..5642)
                     /gene="groL"
                     /locus_tag="VN178_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563800.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWS80219.1"
                     /translation="MAHKELRFNTEARRALEAGVNKLADAVKITLGPKGQYVVLDKKF
                     GSPTITNDGVTIAREIELEDIFENQGAQLVKEVATKTNDVAGDGTTTATVLAQIIVRE
                     GLKNVAAGANPVILRSGVDKAVEAAVEAIKAQSTEISGKEEISRVGAISARSEEVGNV
                     IADAIDKVGKDGVVNVEESQTFGMDLEFTEGMQFDKGYLSPYFVTDQDRMEAVLDDPY
                     ILIANQKIGNVQDVLPLLNQVMQSNKPLLIVSEDVEGEALATLIVNKLRGTFNAAAVK
                     APGFGDRRKRMLEDIAILTGGEVITEELGLKLENTQLNQLGRARKVVITKDDTTIVDG
                     AGETERITGRINQIRSEIETTDSDFDREKLQERLAKLAGGVAVIRVGAATETELKEKK
                     HRVEDALSATRAALEEGIVPGGGVVFLHAQQPVADLLDTLDGDERTGARIIHRALEEP
                     IRQIATNAGADGSIVVDKVRTEGGNVGFNALSGEYEDLVAAGVTDPTMVSRSALQNAA
                     SIGSLIVTTDVIVAEPEEEAPAMPAGGMGGMM"
     gene            5749..6012
                     /locus_tag="VN178_04280"
     CDS             5749..6012
                     /locus_tag="VN178_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80220.1"
                     /translation="MTILSIFRTDRIPSLSRLPKELGREERPCARCGERTEHILYRVP
                     KKVVLLYVKEHSENLHATCVVCARSTVLTGDERDRVLAAFRGE"
     gene            complement(6228..7211)
                     /gene="tsaD"
                     /locus_tag="VN178_04285"
     CDS             complement(6228..7211)
                     /gene="tsaD"
                     /locus_tag="VN178_04285"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWS80221.1"
                     /translation="MILAIETSCDDTCAAIVEPHGRRALSNVVHTQTEHARYGGVVPE
                     VASRAHLERLDGVVQKSLSDAGVSLDEMSRVAVTTRPGLIGALLVGVAAAKGLAYARR
                     LPLVPVNHLEGHVAAAYLTDPGLQPPFVALIASGGHTALYSVDRRMRLLGETLDDAAG
                     EALDKGARMLGLGFPGGPAISRAAHDGDRNRFDFPVGLKSKDNLDFSFSGLKTSLLYK
                     LKSMDGERLRDELPHLAAGYEAAVVEALSRKLLRAVDLHDAPAVVVAGGVAANTLLRR
                     TLEERCGRTGTRLVVPPPELCTDNAAMIGAAAPGTPAVPYPEYLSLNARSV"
     gene            7503..7766
                     /locus_tag="VN178_04290"
     CDS             7503..7766
                     /locus_tag="VN178_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80222.1"
                     /translation="MRTFSLLFAFIALVMLAVFLIYVFRGPAEPLIAAGIFVSFLACL
                     APALLLLKPFEPPEELRRRREEARREREERRRSSDESRRGSGS"
     gene            complement(7732..8184)
                     /gene="rimI"
                     /locus_tag="VN178_04295"
     CDS             complement(7732..8184)
                     /gene="rimI"
                     /locus_tag="VN178_04295"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWS80223.1"
                     /translation="MSENGPHLRRMEARDLPSAMEIDALSLPRPWSAAIWRGELGSPH
                     GLYLVIEDRGEVFGHIGVRHVLGELHITTIAVRPEYRRRGHARALIGAALAAYPNASH
                     VHLEVRPTNVEAIALYESLGFLETGRRPRYYGDEDALLMTLNLDETHH"
     gene            complement(8181..8846)
                     /gene="tsaB"
                     /locus_tag="VN178_04300"
     CDS             complement(8181..8846)
                     /gene="tsaB"
                     /locus_tag="VN178_04300"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWS80224.1"
                     /translation="MLILALDASTPVTTVALARDSGREVLAEISVTASGASETLLPAV
                     HAALHLAGEDLGSVERVLAGVGPGTFTGIRIVAATARALSSGTGIPLSKNSTLDALAA
                     PALAFSRDVLAVLDARRGQVFARRYSEAGPATDIYCLRPEELSVEGGPFVVGDGAVRY
                     RGELSGLGRIPPDGSPLHSVTAVGHVISADLTPVSPEELVPIYVREPDAEVRRVLNPW
                     SRP"
     gene            complement(8840..9262)
                     /gene="tsaE"
                     /locus_tag="VN178_04305"
     CDS             complement(8840..9262)
                     /gene="tsaE"
                     /locus_tag="VN178_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWS80225.1"
                     /translation="MLEELASRVAQVLGPGDVVVLSGEVGTGKSTFVRAAARSLGVTD
                     RVTSPTYQLARGYEGLKDGRPVTVNHMDLYRLEGIEARDVLELEDYLEPDAVTFIEWA
                     DPALGIIENPSVVHIYHETPTTRRISLRGPAAERLTRC"
     gene            complement(9306..9938)
                     /locus_tag="VN178_04310"
     CDS             complement(9306..9938)
                     /locus_tag="VN178_04310"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWS80226.1"
                     /translation="MDFAEAQKEALGCTKCGLCHSRTQVVFGEGPLNAGLFIVGEAPG
                     FNEDKEGKPFSGASGILLDRMLESLDLGREKVYLTTLVKCRPPGSPPRPPKPSEVSSC
                     RPYLVSQLSAVDPRVVVALGDLPSRVLTGRKESVSRIRGRAIPLDGRYVFPTLSLQRA
                     LYTPADRALLVSDFSRLPELLEAERPSTYETLNVTRSPDAEREPVQPGLW"
     gene            complement(9943..10143)
                     /locus_tag="VN178_04315"
     CDS             complement(9943..10143)
                     /locus_tag="VN178_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWS80227.1"
                     /translation="MNIKRARRDRWIRGVCGGVAHTYGWSSNVVRIVTVVIAVIFPGP
                     STLLAFVIYLLLGFILPESEEF"
     gene            complement(10342..10689)
                     /locus_tag="VN178_04320"
     CDS             complement(10342..10689)
                     /locus_tag="VN178_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80228.1"
                     /translation="MFEDLNWKSAARRSAVVIAIYLGLLYLLSVAFPDSSFNMDSRSQ
                     VISLFVNAIIFFFIFTFVYALVERSRNRRIAEARKQKKPGKQTDGDEEAEDSSLKGRP
                     NPNTSRKKTRRRR"
     gene            complement(10673..11788)
                     /gene="alr"
                     /locus_tag="VN178_04325"
     CDS             complement(10673..11788)
                     /gene="alr"
                     /locus_tag="VN178_04325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWS80229.1"
                     /translation="MSSTTAGVGATSRTWAEVDLGAVRHNVATLKRKAPNSRLMAVVK
                     ADAYGHGSVEVARASIEAGADSLAVVTAEEGMELRHAGLEAPILVFTDLLPDRLPLAE
                     AHRLAVTAHSIPSARRIAAHPGLEVHLKVNTGMNRWGVEPSEVGEARKILGPRLAGVY
                     THFASADSDEGATRRQIERFESVLASQPFGGILVHAANSAATLWYPRSHFDCVRPGVA
                     LYGLHPAGDEGDPAAEGLRSAMALKSYVAGVRHLARGDGVSYGLTYRAAAPMCAATVP
                     VGYAEGYRRALSGKAEALIRGQRRPLLGRVTMDACVYGVDEAVEVGDEVVLLGGQGEE
                     RVAAEELGARAGTINYEITTGIDPRRVERSYEDVRGS"
     gene            complement(11798..12277)
                     /locus_tag="VN178_04330"
     CDS             complement(11798..12277)
                     /locus_tag="VN178_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWS80230.1"
                     /translation="MEQQDIRELRVAEISHSEWPTLGPEETVEAAIKLFAESGISGAP
                     VVEDGRLVGIVTEGDLIFRDADIKAPGFLDILGGLIPLGSWDEYRKEALKSAGVTVDE
                     VMTRELITISPHASLAEASTIMADQRVKILPVAEGDTLHSVITRMDILTLHVLNPRR"
     gene            complement(12345..13253)
                     /locus_tag="VN178_04335"
     CDS             complement(12345..13253)
                     /locus_tag="VN178_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HWS80231.1"
                     /translation="MGARARGGALAGGDRLAGGELRCDRSARVPAAGRERVVLRFRLL
                     FVGDVVGPAGCSAVLDLVPQLRRELEPDAVVANAENSAPTGRGITRESGSALLTVTDF
                     LTLGNHAFDADGYKEFLGEEDRVVRPANFGEKNPGLGLGVFEAGGVTVGVTNVLGRVF
                     VERTRVSPFEAADLALEELKARGADLVLVDSHAEATSEKQALGYYLDGRAHAVLGTHT
                     HVPTADHSILPGGTAYATDVGMTGCKESVIGFDREDFLALFLGDWRGISVATRGPVAL
                     NGALVEFDLDARRATRIEAVSREWKP"
     gene            complement(13136..14641)
                     /locus_tag="VN178_04340"
     CDS             complement(13136..14641)
                     /locus_tag="VN178_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWS80232.1"
                     /translation="MKLYTAQEMSRADAGAQELGIPGGVLMERAGAAMAGIALERYSP
                     GRALVVCGGGNNGGDGFVIARELHRSGVDVAVLATKSEYGGDPATNLEILRNLGVPFI
                     ARDDLDAELEMADLLVDALLGTGFSGEVREKEAGIIERMNSTRAPVLAVDVPSGVDGT
                     TGEVQGIAVSADLTVCAHAMKVGCVISPGREHSGEVLAVDIGIPPEADVEPTLVWTDA
                     ASLRGKIPRTAEPAHKYSAGALLVVAGSRGTTGAPVMVVRGAQRTGCGIVFLATSEGA
                     APAVDLALTEALVYGVAEDAGGYMGSGALEEILEHAGRASALVMGPGTGTGDEGRRLL
                     EGILRGAEVPVLLDADAITNLSGTDVLAGRDAPTVITPHAGELGRLLGSGAKEVSARR
                     LDAVCSAADQHRCCVLLKGSDTLVVEGERVAINSTGNVALAAAGTGDVLSGVIGALMS
                     RGMDAYEAARAGAWAHGRAAELWLEEIGWPAESFVATDLLGYLPPAVRELF"
     gene            complement(14638..15036)
                     /gene="acpS"
                     /locus_tag="VN178_04345"
     CDS             complement(14638..15036)
                     /gene="acpS"
                     /locus_tag="VN178_04345"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWS80233.1"
                     /translation="MEGFVVPGIGVDVVDVERMQFALERTPKIRKRLFTEAEIAYCEK
                     YRFAERHYAGRWAAKEAVTKALGCGLIQWNGVEVVRRPRSAPTVRISGKIKKFADAAG
                     VREEDLYISITHSELSAVAVCVVRRERISG"
     gene            15089..15967
                     /locus_tag="VN178_04350"
     CDS             15089..15967
                     /locus_tag="VN178_04350"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWS80234.1"
                     /translation="MDAATDAVIIGNPNSGRAGDEEFLERFAEILRSGDLRVEVLNTE
                     HPNHATELASLAGDRLVVAAGGDGTVNEVLNGLSPGATLGVLPVRTANVLARELGLPL
                     DPYEACRRILEGERSRVDFGVATDSKGAERRFACMAGIGFDAKVISAVTPRLKRYLRT
                     VAFQLTAFKVFFGNRFPTLEILLGDEIHTARFAIIANGQYYGGDYRVSGPGLLTSGEF
                     ETILVERLSDILRPDVFAGIMTRRPLNRTMDSFTSKEVHVRAPGAEVPVQLDGELWGG
                     LPMSFRIEPGALNVVR"
     gene            complement(16017..>16844)
                     /locus_tag="VN178_04355"
     CDS             complement(16017..>16844)
                     /locus_tag="VN178_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWS80235.1"
                     /translation="TVEKIGERACGFTVGDRVGVPWLGCTDGECRYCLSGRENLCENA
                     RFTGYQLDGGYAGYTVAEARFCFPIPEGFPDLQAAPLLCAGLIGHRSLRFAGDAERLG
                     LYGFGASAHIVAQVAAHEGRRIFAFTSPNDDEAQQFARELGAEWAGSSEEAPPEELDA
                     AIIFAPVGALVPAALRTVARGGTVVCAGIHMSDIPSFPYEILWGERSVTSVANLTRQD
                     GLDFLALAPEIPVRTEVVPFPLEEANEALAALRGGRVRGAAVLVVDPAREAMQGSTS"
BASE COUNT         2864 a         5565 c         5361 g         3054 t
ORIGIN      
        1 gctgcaccac cgcctgggcc aagaggtcag ccaactcgcc cgccggtcgg ggtatgctcg
       61 tgatcccgta aaggttcacc gtgtcggcga cctcttcgat gtagtcgaga atgtcatcga
      121 gaccggccgc aagggcggag atatcctcgt ggtcgaatgg ggtgacaaag gtctcgttga
      181 gggtgcgcat ggtctcataa gtgatcttgt caccctggtg ctcgaggtcc ttgatctccc
      241 gccggcgcgc cgggaggttc cccatgtcgt cgagagcctc cttgaggagg acgcccgcgc
      301 tgacgagtat cgcagcctgc tgttcgaaga gatcgtagaa gcgctgctct cgagggacga
      361 gagagagtgc cttgacgttt ttgcccacta tctcgcgaaa ccgtgcgcga gatggaggtg
      421 aggttggcgt tcgtgacgga tcgggcatct ctattctcca atcctgctgg ttttaccccg
      481 ccaggaggga tcatagtgca tcaacttcgc ccttacgttt ctcctccgta aactccagtt
      541 taaatctgtg ttaaatggct gcccagcatg ccagcttgta acgttttgta tgcttcagct
      601 atctctgaca aggcgccgtg ctgaagtgct gataagcgga ggcgaagccg gagcgggctg
      661 aagtgctaac tcattcccac tctatagtac cgggcggctt gctggtgata tccaacgcta
      721 ccctgttgac gccggggacc tcgttgatga tgcggttgct cgtgcgttcg agcaggtcgt
      781 aaggcaggcg ggcccagtca gcggtcatcg cgtcgtctga ggtgacggcc cggatcacta
      841 tagggtaggc gtaggtacga gcgtcgccca ttacgccgac ggagtgtatg gccggcaaaa
      901 cggcgaagct ctgccacagt tctctgtaaa ggcccgcgtt tcttatctcg tcctgcaaca
      961 cggcgtcggc cttgcggagg atctccagcc gctccgccgt cacgtctcct atgaccctga
     1021 tcgcgagccc aggtcccggg aacggctgcc tccagacgag ccgctccggc atcccgagct
     1081 cggccgcgac gacgcgcacc tcgtccttga agaggttcct caatggctcg actagatcga
     1141 ggtccatgac ctcggggagg ccgcccacgt tgtggtggct ctttatcctg gcggcgtcgc
     1201 gggtcccgct ctctatcacg tcggagtaga gcgtgccctg caccaggaag gtggcgtcct
     1261 cgagcttgcc cgcctcctcc tcgaaggtgc ggatgaactc ttcgccgatg atcttgcgct
     1321 tcgcctcggg gtccgtgacg tcggcgagct tgtcgagaaa tcgtcccgcc gcgtccacgt
     1381 ggacgagcgg tacgtcggag ttctgggcga acgcctcgac gacctgctcg gcctcgttgt
     1441 gcctgagaag gccgtggtcg acgaagacgc acgtcagatt gtcgccgatg gccctgtgga
     1501 cgagcatcgc cgccgtcgct gaatcgaccc cgccggagag cccgcagatg gccctcgcct
     1561 ctcctacctg cccccttatc ctctccaccg cgtcggtgat gatgttgacc ggcgtccact
     1621 cgggcctgca gccgcaggcc tcgaagagga agttcttgag gatctccatc ccgtactctg
     1681 tatgcctgac ctcgggatgg aactgtaccc cgaaacggcc ctgcgccccg tcttcgaacg
     1741 cgatgatggg gaccgagggc gtctcggcgg taatctcgaa gccttcaggg agggagagga
     1801 cggcgtcccc gtggctcgtc caggccttct gctcgtcggg cgtgccggcg aagaggccct
     1861 cgtggctctt gacgaccagg tcggagcgac cgtactcccg gatctggacg gcccccacct
     1921 ccccaccctc gacgagcgcc atgagctgca tcccgtaaca gatgccgagc atcggaacgc
     1981 cgaggtcgaa gagatccggc cccacccgcg gagcgccctc ctcgtagacg ctgttgggtc
     2041 cgccggagag gatgatgccc tcgggatctt tcgcccttat ctcgtctacg ggcgtgtcat
     2101 aagggatcat ctccgagtag acgcggcatt ccctgacgcg gcgcgcgatc agctgcgcgt
     2161 actgcccgcc gaaatcgagt accaggatca agagattctc ctgtggagga cctcggtcat
     2221 cttcccattc cgacgccctg ggagcgctgc tcggtcttgc cctcggtggc cagcgacggc
     2281 gcgtacatga cctcggcctt ctggaattcc tttatgttct ggtagccggt cgtcgccatg
     2341 ctcgtcctca gggcgcccat gaggttgagc gtcccgtcgt tctcgcgggc gggcccgacg
     2401 agtatttctt cgagacttcc cgtggtcctc gtgctgatac gggtgccgcg cgggagggtg
     2461 gggtggaacg tggccatccc ccacgagtag ccgcgacctg gggcctcctc ggccctggcg
     2521 aaggcgctgc ccagcatcac ggcgtcggcc ccgcaggtta tggccttggc gatctcaccg
     2581 ccggtgcgca tcccgccgtc ggcgatgacg ttcacgtact cgccggtctc caggtagtgg
     2641 cgcatgcggg cggccgcggc gtcggccacg gccgtagcct gcggcacccc caccccgata
     2701 acgccccgcg tcgtgcagat gcgccctggc cccacaccga ccaggacgcc gacggcgcct
     2761 gtccgcatga ggtggagcgc cgtcgagtag ctcgcgcagc cgccgactat aacgggaacg
     2821 ttcagggacg gcacgaactc catgaggttc agcggttcga cggacctgga gacgtgctcg
     2881 gcggagacca cggtcccctg gaccacgagt acgtcgaggc cggcctctat ggccgctctg
     2941 tggtaacgct cgacccgctg gggcgtaagc gaggccgccg ctatgacgcc cttgtccttt
     3001 atctcctgca cccggcggaa gatgagctct tctttgatcg gctcggcgta gatatcctgc
     3061 attatcctcg tggccttgtg ctcgggcagg ctcgctatct cctcgaagac gggaccggga
     3121 tcctcgtacc tcgtctgcag tccctcgagg ttgagcacgg cgagcccgcc cagagacccg
     3181 atgatccccg cggtcgtggg atctacgacc gcgtccaggg cgctcgccag gcatggcagg
     3241 tcgagattca ggttgcctaa agcccaggag atatctacgt cctcggggtc gcgggtcctt
     3301 cgactcggta ctatggcgat ctcatcgagc ccgtaggccc tgcgtgcagt cttgcctctc
     3361 ccaatctcaa tatccatgcg ggtgccgagg ttcctttccg gtcgttgtca gtagatagaa
     3421 cgaagcccac ggagaaccgc ctccgtgggc ctcgatacac ttttcttccg gacgtagcaa
     3481 taggggctat gctacaccgc gaacgccgcc cgggtcaagc agctaacccg gcctagagtc
     3541 ccggtattat ccccagaaag gccagcgcga ccaccacgac aatgacaacc actatgatga
     3601 caaccaggtt gttgttatcc atctaccgac ctccccggag acctatcctg cgccagaaag
     3661 cactgtctcc tcggcggagc ctcactagag gagccgcgag agcagcagca agatcaagag
     3721 aatcaaaaga atgaccacaa gtataggtat gagcccaccc cgcactttct cacctccctc
     3781 gccagagatc tttccctaac taacgaacgg caacacgagc acgaccagaa gaatcaccac
     3841 cagtacgcca acgactatgg cgagtcccct atctatcctc cctcacctca ctccccgtct
     3901 ctaggtggcc ggctacgtcg caatatatac cttctcagac ctagacgaaa caaaatcggt
     3961 tacgaaaaag cggcgcccct ctcgggacgc cgcacataga agctcttctg gagagctcct
     4021 tacatcatgc cgcccatgcc gcctgctggc atggcgggcg cctcctcctc gggctcggcc
     4081 acgattacat ccgtggtcac gatgaggctg ccgatggagg cggcgttctg cagggcggag
     4141 cgcgagacca tcgtcgggtc ggttacaccg gccgctacta ggtcctcgta ctcgccactc
     4201 agggcgttga agccaacatt gccgccctcg gtgcggacct tgtccaccac gatggagccg
     4261 tcggccccag cgttcgtggc gatctggcgg ataggctcct ccagcgccct gtggatgatc
     4321 ctggcaccgg tccgctcgtc accgtccaat gtgtcgagca ggtcggccac aggctgctgg
     4381 gcgtgcaaga acaccacgcc accgccaggc acgatgccct cctccagggc cgctcgggtc
     4441 gcggagagcg cgtcctcgac gcggtgcttc ttctccttga gctccgtctc ggtcgcggcg
     4501 ccaaccctga tgacggccac gccgccggcc agcttggcga gccgctcctg gagcttctcg
     4561 cggtcgaagt ccgagtcggt agtctcgatc tcggaacgta tctggttgat acggccggtg
     4621 atcctctcgg tctcgccggc accgtcgacg atggtggtgt cgtccttggt gatgaccacc
     4681 ttgcgggcac gcccgagctg gttgagctgg gtgttctcca gcttgaggcc gagctcctcg
     4741 gttatgacct cgccgccggt caggatggcg atgtcctcca gcatcctctt gcgcctgtcc
     4801 ccaaagccag gagccttgac ggcggccgcg ttgaacgtgc ccctgagctt gttgacgatc
     4861 aaagtggcca gagcctcgcc ctccacgtcc tcggagacga tgaggagcgg cttgttggac
     4921 tgcatgacct ggttgagtag cggcaggacg tcctggacgt tgccgatctt ctggttggcg
     4981 atcaggatgt agggatcgtc gagcacagcc tccatgcgat cctggtcggt gacgaagtac
     5041 gggctcagat aacccttgtc gaactgcatc ccctcggtga actccaggtc catcccgaag
     5101 gtctgcgact cctcgacgtt cacgaccccg tccttgccga ccttgtcgat ggcgtcggcg
     5161 atgacgttgc cgacctcctc agagcgcgca gaaatagccc ccacgcgcga gatctcttcc
     5221 ttgccgctga tctcggtgga ctgggccttt atcgcctcga cagccgcttc cacagcctta
     5281 tcgacaccgc tgcgcaagat gacagggttg gccccggcgg caacgttctt gagaccctcg
     5341 cggacgatga tctgggccag aacggtcgcc gtcgtggtcc cgtcaccggc cacgtcgttg
     5401 gtcttggtcg cgacctcctt gacgagttgg gcaccctggt tctcgaagat gtcctccagc
     5461 tcgatctccc gcgcgatcgt cacaccgtcg ttcgtgatcg tcggcgaacc gaacttcttg
     5521 tcgagcacca cgtactggcc cttgggacct agcgtgatct tgaccgcgtc agcgagcttg
     5581 tttacgcccg cctccagcgc ccgacgcgcc tcggtattga accttagctc tttgtgcgcc
     5641 actatctgcc tcctctgcta aactaggacg cgatttcaac tgcctggcac tctcttggtt
     5701 agagtgccga tgcacagagt atatgcctgg tggggttaca attcaaacat gaccatccta
     5761 agcatcttcc gcaccgacag gataccgagc ctgagccgtc tgcccaagga gttgggccgc
     5821 gaagaacgcc cttgcgcgcg ctgcggcgag cggaccgagc acatcctcta cagggtcccc
     5881 aagaaggtgg ttctgctcta cgtcaaggag cattcggaga atctgcacgc gacctgcgtg
     5941 gtatgcgcga gaagcaccgt acttaccggc gacgaacgcg accgagtact cgccgcgttt
     6001 cgcggcgagt agcatttcag cacttcagca cgcttcgagc ctacggctct cgctcgtcag
     6061 catttcagct tattgtctgc ggattgccca ggctactgga gcgggcccat agatgtggac
     6121 ccaccgcgaa tcacgcttga ccacgggcgg tgggccacaa gcgaaagcaa aaagctgact
     6181 agctgaaatg cgcgaatggg gaaaggctcg cccttcccca tccgccctca gacgctgcgg
     6241 gcgttgaggg agagatactc ggggtaaggg acggcgggag tgcccggggc ggcggccccg
     6301 atcatggccg cattatcggt gcatagttcc ggtggaggga cgacgaggcg ggttcccgtc
     6361 ctcccgcagc gttcttcgag ggtacggcgc aggagggtgt tggcggcgac gccaccagcc
     6421 acgacgacgg ctggcgcgtc gtggagatct acggcccgaa ggagcttgcg ggagagggcc
     6481 tcgaccacgg cggcctcata accggcggcc aggtgcggca actcatcgcg gaggcgttcc
     6541 ccgtccatcg acttgagctt gtacagcagg ctcgtcttca ggccggagaa gctgaagtcg
     6601 aggttgtctt tgctcttcag gccgacgggg aagtcaaagc ggttccggtc tccgtcgtgg
     6661 gcggctctag agatagcggg gccgccgggg aaaccgaggc ccagcatcct ggcgcccttg
     6721 tccagtgcct cgcctgcggc gtcgtcgagc gtctctccga gcaggcgcat cctgcggtcc
     6781 acggagtaga gggccgtatg cccgcccgag gcgatcaggg cgacgaacgg cggctgcagg
     6841 ccggggtccg taaggtacgc ggcggcgacg tggccttcca ggtggtttac ggggaccaga
     6901 gggagtctgc gggcgtaggc cagtcccttc gcggccgcaa ccccgacgag gagcgccccg
     6961 atcaaacccg gccgcgtggt gaccgccaca cggctcattt catcgagaga aacaccagcg
     7021 tcagaaagcg acttctgcac taccccatcg aggcgttcga ggtgggcgcg ggaggcgact
     7081 tcggggacta cgccaccgta gcgcgcgtgc tccgtctgcg tgtggaccac gttcgagagg
     7141 gcacgccgcc cgtggggctc gacgatcgca gcacaggtgt cgtcacagga ggtttcgatg
     7201 gccaggatca tggtttgttt atagcattcg ggggcgtgtc gaatagtgaa ggtggaattg
     7261 tgaatcggga aagggatggt tgagggatca ataccgaatt cctatctcac atcgaatccg
     7321 ggtgaatggc gactgcaaag cgggccgtca cagcacgggc ccacctcttg tgccaagcgt
     7381 ggattcccgc ttgcgcggga acgacgtatg agaaggtcca gtgccccaca agccgcaaga
     7441 cgctaccgtt cagccggatt cggtatcaca actcacgatt ccatcgggat atacttggtg
     7501 ttatgcggac gttctcgctc cttttcgcct ttatagcgct ggtgatgttg gcggtgtttc
     7561 tcatctacgt gttcaggggg cctgccgagc ctttgatcgc ggccggcata ttcgtctcct
     7621 ttctagcctg cctcgcccct gcgctgctgc tgctgaagcc tttcgagccg ccggaggagc
     7681 tacggcgccg cagggaggaa gcccgcaggg aacgcgagga gcgaaggcgt tctagtgatg
     7741 agtctcgtcg aggttcaggg tcatgagcaa tgcgtcctcg tccccgtagt agcgaggcct
     7801 gcggcccgtc tccaaaaagc cgagcgactc gtagagcgcg atggcctcca cgttggtcgg
     7861 gcgtacctcg aggtggacgt ggcttgcgtt cggataggcg gcgagcgccg cgccgatcag
     7921 ggcgcgggcg tgtccgcgcc tgcggtactc cggtcttacg gcgatggtgg ttatgtgcag
     7981 ctcgccgagg acgtgacgga ccccgatgtg gccaaagacc tcgcccctgt cttcgatcac
     8041 caggtagagc ccgtggggac taccgagctc tccgcgccag atcgccgcgc tccagggacg
     8101 ggggagggac agagcgtcta tctccatagc ggaaggaaga tccctggcct ccatccggcg
     8161 caggtgcggc ccattctcgc tcaagggcgg ctccaggggt tgaggacgcg acggacctcg
     8221 gcgtcgggtt cgcgcacgta gatggggacg agctcttcag gactcacggg ggtgagatcg
     8281 gcagagataa cgtgcccgac cgcagtgaca ctgtggagcg gggatccgtc cggcggtatg
     8341 cgcccgagtc cggagagctc cccgcggtag cggacggctc cgtcgccgac cacgaacggt
     8401 cctccctcca ccgacagttc ctcgggcctg agacagtaga tgtccgtcgc ggggccggcc
     8461 tccgaatatc tgcgggcgaa gacctgtcct ctcctggcgt cgagcacggc cagcacgtcc
     8521 cgcgaaaacg cgagcgcagg agcggccagg gcgtccagcg tggagttctt cgacagtggg
     8581 atgccggtac ctgaggagag tgcgcgggcc gtcgccgcaa ctatgcggat gcccgtgaaa
     8641 gttcccggtc ctaccccggc gagcacgcgc tccacggagc cgagatcctc ccccgcgaga
     8701 tggagggcgg cgtgtacggc gggaagcagt gtttccgagg ccccgctggc ggttacggag
     8761 atttcggcga gcacctcgcg gccgctatcg cgggcgaggg cgacggtggt taccggggtc
     8821 gaggcgtcga gggctagaat cagcaccgtg taagccgctc cgcggctggt cctctgaggc
     8881 tgatgcgccg ggtggtcggg gtctcgtggt agatgtggac gaccgagggg ttctcgataa
     8941 tgccgagggc gggatccgcc cactcgataa acgtcacggc atctggttcc aggtagtcct
     9001 ccagctccag tacgtccctc gcttcgatcc cctccagtcg gtagaggtcc atgtgattga
     9061 cggtcaccgg tcgcccgtcc ttgagtccct cgtagccgcg ggcgagctgg taggtcgggc
     9121 tcgtcaccct gtccgtgacg ccgagggatc tggcagcggc ccgcacgaag gtgctcttgc
     9181 ccgtgccgac ctcgccggac agcaccacga cgtcaccagg acctagaacc tgcgccaccc
     9241 tggacgccag ctcctcgagc atagcctcct ttaggccttc gagctccacg ggaaaccctg
     9301 aaactctacc agagccccgg ctggactggt tcccgctccg cgtcggggga gcgggtgacg
     9361 ttgagggtct cgtaggtgga gggccgttcg gcttcgagga gctcgggcag gcgggagaag
     9421 tccgagacga ggagcgcccg gtccgccgga gtgtagaggg cgcgctggag ggagagcgtc
     9481 gggaagacgt agcggccgtc gagcgggatc gcacgccccc tgatgcgcga gacggattct
     9541 ttcctccccg tgagtacccg gctgggcaga tcccctagcg caacgacgac cctggggtcg
     9601 acggccgaga gctgggagac caggtaaggc cggcaggagc tcacctcaga aggcttcggt
     9661 ggccgggggg gagatccagg cggacggcac ttgaccagcg tggtcaggta gaccttctcg
     9721 cgcccgaggt cgagcgactc gagcatgcgg tcgaggagaa tccccgaggc tcccgaaaag
     9781 ggtttgccct ctttgtcctc gttgaagccg ggcgcctcgc cgacgatgaa cagcccggcg
     9841 ttcagcggcc cctcgccgaa gacgacctgc gtcctagagt ggcacagccc gcacttcgtg
     9901 cagcccaggg cctccttctg ggcctccgcg aagtccaagc gcctagaact cctcgctctc
     9961 ggggaggatg aagccgagca acaggtagat cacgaacgcc aaaagcgtgc tcggaccggg
    10021 gaagatcacg gcgatcacca ccgtaacgat ccgcactaca ttagagctcc agccgtaggt
    10081 gtgggccacg ccgccgcaga cgccccggat ccaacgatcc ctcctggccc tcttgatgtt
    10141 caagacccct acctcctccg gccccgcgag tcgctacgct ctcttaagcg attctacacg
    10201 accgcgacct ggatcttcca cagcaagccg agggccatga gcagcctgag ataaccggcc
    10261 gcgtacccga cgtcggcgcc cgacgccgat gagcccgacg gtatccagcg ggggctcgag
    10321 ggccgcgcgg aggtccaacg attagcgccg gcggcgggtc ttcttgcggc tcgtattggg
    10381 gttgggacgg cctttgagcg aggagtcctc tgcctcctcg tcgccatcgg tctgctttcc
    10441 cggcttcttc tgtttacggg cctcggctat gcgcctgttt ctgctgcgct ccacgagcgc
    10501 gtagacgaac gtgaagatga agaagaatat gatggcgtta acgaacaggc tgatgacctg
    10561 tgacctggag tccatgttga agctgctgtc ggggaacgcc acgctgagta ggtagagcag
    10621 gccgaggtag atcgctatga ctaccgccga cctcctggcg gctgacttcc agttaagatc
    10681 ctcgaacatc ctcgtagctc ctctcgacgc ggcggggatc tatccccgtg gtgatctcgt
    10741 agtttatcgt gcctgcgcgt gcgccgagtt cttcggccgc tacgcgctct tcgccctggc
    10801 cgccgagcag cacgacctcg tccccgacct cgacagcctc gtccaccccg tagacgcagg
    10861 cgtccatcgt gacccgcccc agcagcgggc gcctctggcc ccggatcagg gcttccgcct
    10921 tacccgagag cgcccgccga tacccctccg cgtacccgac aggcacggtc gccgcgcaca
    10981 tcggcgccgc ggccctgtag gtcagcccgt aggatactcc gtcacccctg gccagatgcc
    11041 gtacgccggc tacgtagctc ttgagggcca tcgcagaccg cagaccctcc gcggcaggat
    11101 ctccctcgtc gccagcaggg tgcagaccgt agagcgccac gccgggacgg acgcagtcga
    11161 agtgcgagcg aggataccag agggtcgccg ccgagttagc agcgtgcacc aggatacccc
    11221 caaagggttg tgaggcgagg acagattcga agcgttcgat ctggcgtctc gtcgccccct
    11281 catcggagtc ggccgaggcg aaatgcgtgt agacgccggc gagcctgggg ccgagtatct
    11341 tgcgcgcctc tcccacctcc gaaggctcca caccccagcg attcatcccc gtattcacct
    11401 tcaggtggac ttcgagaccg gggtgggccg cgatgcgcct cgcgctcggg atggagtggg
    11461 ccgtcacggc caggcgatgc gcttcggcca gcggtagcct gtcgggcagg agatcggtga
    11521 agaccaggat cggggcttcc aggcccgcgt gccgcaactc catcccctcc tcggcggtga
    11581 cgacggctag agagtctgcc ccggcctcga tgctggcgcg ggccacctcc acggagccgt
    11641 ggccgtaggc gtccgccttg acgacggcca tcaggcgcga gttcggggcc ttgcgtttga
    11701 gggtggccac attgtggcgc acggcgccca gatccacctc cgcccaggta cggcttgttg
    11761 ccccgactcc ggcggtcgtg ctactcaccg atcgttctta ccggcggggg ttcagtacat
    11821 gcagggtcag aatgtccatg cgcgtgatca cactgtggag ggtgtcgccc tctgcgacgg
    11881 gtaggatctt gaccctctgg tcggccatga tcgtggaggc ctcggcgaga gaggcgtggg
    11941 gagagatcgt gatcagctcc ctcgtcatca cctcgtcgac ggtgacgccg gcgctcttga
    12001 gcgcttcttt gcggtattcg tcccaactcc ccagcgggat aagaccaccc aagatgtcga
    12061 ggaagcccgg tgctttgatg tccgcgtcgc ggaagatcag atccccctcc gtgactatgc
    12121 ccacgagcct gccgtcctcg acgacggggg cgcccgagat gcctgactcg gcgaagagct
    12181 tgatcgcagc ctccaccgtc tcctcaggcc ctagcgtggg ccactccgag tgcgagatct
    12241 ctgccaccct caattccctt atatcctgct gctccatgct ccatcccctg atcgtcgttc
    12301 ccggtcgcac caccgctatt gtaaacggcc cggcgagcgt ccggtcaagg cttccactcc
    12361 ctgcttacgg cttctattct cgtagcccgc cgggcgtcga ggtcgaactc gacgagcgcg
    12421 ccgttgagcg cgacgggacc gcgggtggcg accgagatcc cacgccaatc gcccaggaac
    12481 aaagcgagaa aatcttcccg gtcgaacccg ataacgctct ccttgcaacc ggtcatgccc
    12541 acgtctgtcg cgtaggcggt tccgccgggc agtatggaat ggtccgcggt gggcacgtgc
    12601 gtatgggtgc ccaaaacggc gtgcgccctc ccgtcgagat aatagccgag cgcctgcttc
    12661 tcgctcgtgg cttcggcatg ggaatcgacg agcacgaggt cggcgccgcg ggccttcaat
    12721 tcctcgaggg ccagatcggc agcctcgaag ggcgagactc tcgtcctctc gacgaacacg
    12781 cgccccagca cgttcgtgac gcccacggtt accccgccag cctcgaaaac gcccaggccc
    12841 agcccagggt tcttttcgcc gaagttggcg ggacgcacca ccctgtcctc ctctccgagg
    12901 aactctttgt atccatcggc gtcgaaggcg tggttcccga gggtgaggaa gtcggttacg
    12961 gtgagcaagg cggagccgct ctcgcgggtg ataccacgcc ccgtcggggc cgagttctcg
    13021 gcgttggcga cgacggcgtc aggctcgagc tctcgccgca actgaggtac gagatccagg
    13081 accgccgagc atcccgcggg ccctacgaca tccccgacga agagaagcct gaacctcaga
    13141 acaactctct cacggccggc ggcaggtacc cgagcagatc ggtcgcaacg aagctctccg
    13201 ccggccagcc tatctcctcc agccagagct ccgccgcgcg cccgtgcgcc caggcgccgg
    13261 ccctggccgc ctcataagcg tccatcccgc gagacataag cgccccgatc acaccggaca
    13321 acacgtcccc tgtcccggcc gccgcgagcg cgacgttccc cgtggagttg atggcgactc
    13381 tctcgccctc tacgacgaga gtatcggaac ccttgagcaa cacgcagcaa cggtgctgat
    13441 cggccgcact gcaaaccgca tcgagcctgc gggccgacac ctccttggcc cccgatccaa
    13501 gcagacgccc gagttcaccc gcgtgcggtg ttatgacggt cggcgcgtct ctccccgcga
    13561 gaacatcagt gcccgaaagg ttcgtgatgg cgtcggcgtc gagcaacacg ggcacctcgg
    13621 ccccgcgcag gataccttcg aggaggcgcc tgccctcatc ccccgtcccg gttcccgggc
    13681 ccatcacgag cgcagaggcc ctccccgcgt gctccaggat ctcctccagc gcccccgacc
    13741 ccatgtagcc tcccgcgtcc tcggcgacgc cgtagaccag tgcttcggtt agcgcgaggt
    13801 cgacggctgg cgcggccccc tcggaggtgg cgaggaagac tatgccgcac cccgttctct
    13861 gcgcgcccct gacgaccata acgggggctc ctgtcgtgcc gcgggagccg gcgacgacga
    13921 gcagggctcc ggcggagtac ttgtgggccg gctcggcggt acgcggtatc ttcccccgca
    13981 gcgaggccgc atcggtccag accagggtcg gttcgacgtc cgcttccgga gggatgccta
    14041 tatccacggc gaggacctcg cccgagtgct cgcgcccggg ggagataacg caccccacct
    14101 tcatggcgtg cgcgcagacc gtaagatcgg cggacacggc gataccctga acctcacccg
    14161 tcgtcccgtc cacgccggag ggcacgtcga cggcgagtac cggagcccgc gtggagttca
    14221 tccgctcgat gatcccggct tccttctccc gtacctcccc cgaaaacccg gtcccgagca
    14281 gggcatcgac gaggaggtct gccatttcga gctccgcgtc gagatcatcc ctggctataa
    14341 acggcacccc caggttccga agtatctcca ggttggtcgc cgggtccccc ccgtactcgc
    14401 tcttcgtcgc cagaaccgcg acgtcgacgc cggagcggtg cagttcgcgg gcgatgacga
    14461 agccgtcacc gccgttgttg ccgccaccgc agacgacgag ggcccgccct ggggagtacc
    14521 gctccagggc gataccggcc atggcggcgc cggccctttc catcagcacg ccgccgggga
    14581 tgccgagctc ctgcgccccg gcgtcggcgc gggacatctc ctgtgcggtg tagagcttca
    14641 ccctgagatc cgctcccgtc gcacgacgca gacggcgacc gcggagagct cggagtgggt
    14701 tatcgagatg tagaggtctt cctcgcgcac gccggccgcg tcggcgaact tcttgatctt
    14761 gcctgagata cggaccgtcg gggcgctgcg aggcctccgc acgacttcga ctccgttcca
    14821 ctggatcagg ccgcacccca aagccttggt aacggcctct ttggccgccc agcgtccggc
    14881 gtagtggcgc tcggcgaagc ggtacttctc acaataggcg atctcggctt cggtaaacag
    14941 gcgcttacgt atcttcgggg tgcgctccag ggcgaactgc atcctctcga cgtcgacgac
    15001 gtcgaccccg atccctggta cgacgaaacc ttccacgggg cgtatgttac ggcagattca
    15061 ccgctggcaa aagggctata gtgtatggat ggacgcggcc acagacgctg tcatcatcgg
    15121 taatccgaat tcgggacggg cgggcgacga ggaatttctg gagcgtttcg cggagatcct
    15181 gcgctcggga gacctgaggg tggaggtcct caacacggag catcccaacc acgccaccga
    15241 gctcgcatcg ctcgccgggg accgtctggt cgtggccgcc gggggcgacg gcacggtcaa
    15301 cgaagtcttg aacgggctct ctcctggggc cacgctgggt gtactgcctg tccgcacggc
    15361 gaacgtgctg gctcgggagc tcgggctgcc actcgacccg tacgaagcct gccggcgcat
    15421 tctggaaggc gagcgctcca gggtggactt cggcgtcgcc accgacagta agggagcaga
    15481 gcgccgcttc gcctgtatgg ccgggatagg cttcgacgcg aaagtaatca gcgccgtgac
    15541 accgcggctg aagcgctacc tgcggacggt ggcattccag ctaacggcgt tcaaagtgtt
    15601 cttcgggaac aggttcccca cgctggagat actcctcggc gacgagatcc acaccgcgag
    15661 gttcgccata atagccaacg gccagtacta cggtggcgac tacagggtgt cagggccagg
    15721 tttgcttacg agcggcgagt tcgagaccat cctggtcgag cgcttgagcg acatccttag
    15781 gcccgacgtc ttcgccggga tcatgaccag aaggcccctc aaccgcacga tggactcctt
    15841 cacctcgaag gaggtccacg tcagggcccc gggcgcggag gtgcccgtac aactcgacgg
    15901 cgagctctgg ggcggactgc ccatgtcctt ccgcatcgaa ccaggcgccc tgaacgtagt
    15961 acgttagcat ttcagcacgc tcaggcttcg ccttcgctcg tcagcacttc agcttgtcag
    16021 cttgtactgc cttgcatcgc ctctcgagca gggtcgacga cgagcacggc ggcgccccgt
    16081 accctgccgc cgcgtagggc tgctagcgcc tcgttggcct cttcgagggg gaagggtacg
    16141 acttcggtgc ggacgggtat ttctggggcg agggcgagga agtccaggcc gtcctggcgc
    16201 gtgaggttgg ccaccgaggt cacggagcgc tctccccaga ggatctcgta tgggaaagag
    16261 ggaatgtcgc tcatgtgtat gcccgcgcag accacggtcc cgcccctggc gacggtcctg
    16321 agagcggcgg ggacgagggc gccgacgggc gcgaagatga tcgccgcgtc cagttcctcg
    16381 ggtggggcct cttcggagct gccggcccat tcggcgccca actccctggc gaactgctgt
    16441 gcctcatcgt cgttcgggct ggtgaaggcg aagatgcgcc ggccctcgtg cgccgcgacc
    16501 tgggccacga tgtgggccga ggccccgaag ccgtagagtc cgagcctttc tgcatcgccg
    16561 gcgaagcgca acgagcggtg cccgatcagg ccggcgcaca acagcggcgc ggcctgcagg
    16621 tcggggaaac cctcggggat cgggaagcag aaacgggcct cggcgacagt gtaacctgcg
    16681 tagccaccgt cgagctggta cccggtgaac ctggcgttct cgcagaggtt ctcgcggccg
    16741 gagaggcagt agcggcactc gccgtcggta cacccgagcc acggcacccc gaccctatcc
    16801 cctaccgtaa agccgcatgc gcgctcgccg atcttctcca ccgt
//
LOCUS       DATMVF010000094         6991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_54740, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000094 DATMVF010000000
VERSION     DATMVF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54740"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1969
                     /locus_tag="VN178_04360"
     CDS             <1..1969
                     /locus_tag="VN178_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWS80236.1"
                     /translation="SAIELLDDVAIGRARANPAYRDSTRFVKGDPKAVLVVEWSGTKE
                     ELDERFARLDDLAGEVGALAATPLRTKEEMGQTVLLRKSTLPLLLGTTDKEKPVAFVE
                     DAAVPPDRLEEFIGRFEEIVEKNGTWACFYGHASVGCLHVRPALDTSDPDGVSRMRRI
                     AEEVADLVVDCGGSISGEHGDGLSRSEFVQKMYGEEILRAFADVKRLFDPEGMMNPGV
                     IVDPPPMDRQLRIGPGRERLPIRTNLSFEDQGGFAKAVELCNGSGLCRKRTGGTMCPS
                     YMVTLDEQDTTRARANMLRSVIEGTLPPEELTGRRMKEVMDLCVGCKACKTECPSRVD
                     VASMKTEVLYQTGSVHGFSLRQKVAGHIRRQLALAALVPSLYNAAAGTRLARKAAALA
                     GIDPRRSLPRVAKKTFSKRFPELPNGAGPEEVALFNDTWNEYQRPEVGEGAVRLFAAA
                     GARIHLPEVVCCGRPMLSEGLVDEARENARRNLDLLMPLVERGVPLVGLEPSCILTLR
                     DDYRKLLPDDERVDRLARATRLFEEAMLDLDTELPLREGTPVLLHGHCHQKALVGTGP
                     TERLLGFAADVEVVDSGCCGMAGLFGYEKGHYEVSMKMGERRLFPAVREAGERVVVAP
                     GTSCREQILDGTRRRALHPAEYLASLLDGPS"
     gene            1976..2788
                     /gene="nudC"
                     /locus_tag="VN178_04365"
     CDS             1976..2788
                     /gene="nudC"
                     /locus_tag="VN178_04365"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HWS80237.1"
                     /translation="MAELEKALWFAFRGERMLVLEDAPVRVPLADSLDELGLDVLFCL
                     EIGELGGHACWAVEVRADARPPEGMVFEDLRALFYRVDEEFFRMAGRAKQIVGWYATH
                     RFCGRCGGETKPAPVEFAMRCSRCGMMHFPRLSPAAIVLVRRGDRILLAHSPGFPKGL
                     YSVLAGFVEPGESIEEAIVREVREEVGIEVTNVRYFGSQPWPFPNSLMIGFTADYMDG
                     ELNPQPGEIEDAGWYTAEDLPQLPPRISIARAMIDDFVRQQGLDPEGLKIPG"
     gene            complement(2960..5212)
                     /locus_tag="VN178_04370"
     CDS             complement(2960..5212)
                     /locus_tag="VN178_04370"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HWS80238.1"
                     /translation="MTRRKFIWLAGIGVAGMALLGGAYALWRRPARISGAIAGVERDV
                     SRGPHRIGAFTVVLETGPSLSDVVLSVTHSSRPDRILWQSTPGTSFLAAAEGEETVRE
                     SSGHFFIDDEIESLHSNQTIDYIEKHQSILVVGGRLTRGGDSEGVDYTLTFLPVSEER
                     LRFEIEVEEPYNRVYLTYASSPEERFFGFGTQYTYFDMKGHKVPIFIQEQGIGRGKQP
                     ITLAADWKADAGGSPYTSGASVPHYITSQMRSVFLENYEYSSFDLRERNRVQVEVFSS
                     QMTGQILSGETPVELIELYTEYSGRMRLLPEWILSGAVVGLQGGTDKVLDEYDKLKAL
                     GTPVAALWLQDWVGQRETDFGMQLWWNWELDEVHYPGWDRLREQLERDDVRLMTYIGP
                     WLADDVEQEKPNYRRNLFEEAARKGYLVKNREGEPYRVKTTDFRAAFVDLTNPAARDW
                     IKDVIKEELIGNGASGWMADFGEGLPYDAVLFSDADPKSYHNRYAEEWAEVNREAIRE
                     AGRGEDIVFFNRSGYTRSPGESTLFWLGDQLVDWDEHDGIKSAVTGLLSSGLSGYSLE
                     HSDIGGYTAIDNFLLKYHRSNELLMRWIELGAFTTVFRTHEGNRPKVNHQIYSDEETL
                     RHFSRFAKVYTAWKPYRIELVKEASETGLPVIRHPFVHYPDDPEVLGLDYQFMVGRNL
                     MVAPVLDPGEEIVEVYLPAGRWVHLWSGRKYGSLDKGAYETVHAPVGEPAVFYEDRSD
                     VGRWFRESEQ"
     gene            5685..6005
                     /locus_tag="VN178_04375"
     CDS             5685..6005
                     /locus_tag="VN178_04375"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80239.1"
                     /translation="MCSYPKIRHRPTQPDVLLPQFMVGPELMFAPVLDPGEDSVGVYL
                     PAGRWVHFWTGKRFGSPQRGVNETVPAPIGEPAVFYKEGSAEGTRFRKCSNAGDCYDA
                     AEVE"
     gene            6034..6426
                     /locus_tag="VN178_04380"
     CDS             6034..6426
                     /locus_tag="VN178_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWS80240.1"
                     /translation="MKRENVTSGTVWESRVGYSRAVRRGRFVYVSGTTATDGSGGYVG
                     VGDPYAQTVQAINNIRVALQAVGADLSDVVRTRIYVVNIEDWEKIGSAHGECFGNVRP
                     ATSMVEVSRLIAPEILVEIEADALIDES"
     gene            complement(6625..>6991)
                     /locus_tag="VN178_04385"
     CDS             complement(6625..>6991)
                     /locus_tag="VN178_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80241.1"
                     /translation="IRIELAVINTRVLARGVVNAIRRGDRIAPQLPEAVLDLSRSVRA
                     LAAFLEDGGGPEEARHWALEAARTATEILEDRHELGISVLVGQVRSAAVDLLRSTGMD
                     QASALQALEDAAGRASEIG"
BASE COUNT         1339 a         2155 c         2159 g         1338 t
ORIGIN      
        1 atcggcgata gagttgctcg acgacgtcgc cataggccgc gcgagggcca accccgccta
       61 cagggactcg acccgatttg taaaggggga tcccaaggcc gtcctcgtcg tggagtggag
      121 cggtacgaag gaagaactgg acgaacgctt cgcaagactc gacgatctag ccggcgaggt
      181 cggggcgctg gccgcgacgc ctctgcgcac caaagaagag atgggacaga cggtcctgct
      241 ccgcaagtcc acgctaccac ttctgctcgg tactacagac aaagagaaac cggtcgcgtt
      301 tgtggaggac gcggccgtgc cgccggaccg gctggaggag tttataggcc gcttcgagga
      361 gatcgtcgag aagaacggta cctgggcctg cttctacggc cacgccagcg tggggtgcct
      421 gcacgtcagg cctgctctgg acacgtccga tcccgatggc gtctcgcgga tgcggcgtat
      481 cgccgaggag gtcgcggacc tcgtcgtcga ctgcggcggg tccatctccg gtgagcacgg
      541 cgacggcctc tcgcgttcgg agttcgtgca gaagatgtac ggggaggaga tcctgcgcgc
      601 ttttgcggac gtcaagcgtc tcttcgaccc cgaggggatg atgaaccctg gcgttatcgt
      661 agatcctccg ccgatggacc ggcagctgcg catcggcccc ggccgcgagc gcctccccat
      721 aagaacgaac ctctccttcg aggatcaggg cggcttcgcg aaggccgtag agctctgcaa
      781 cgggagcggt ctctgccgca agaggacggg gggcacgatg tgtccttctt acatggtgac
      841 cctcgacgaa caggacacca cgagggcgcg ggccaacatg ctcaggtccg tgatcgaagg
      901 caccctcccg ccggaagagc tcaccgggcg gcgcatgaag gaggtcatgg acctctgcgt
      961 cggatgcaag gcgtgcaaga cggaatgccc ctcccgcgtc gacgtcgcga gcatgaagac
     1021 cgaggtcctc taccagacgg gcagcgtaca cggcttttcc ttgcgccaga aggtggcagg
     1081 acacatccgc cgccagctcg cgctcgcggc cctggtcccc tcgctctaca acgccgccgc
     1141 aggaacccgc ctcgcccgca aagccgccgc cctcgcgggc atagaccccc gtcgctcctt
     1201 gccccgcgtg gcgaagaaaa ccttctccaa gcgcttcccc gaactgccca acggcgccgg
     1261 gcctgaagag gtcgccctct tcaatgacac ctggaacgag taccagaggc ccgaggtcgg
     1321 agagggcgcc gtaaggctct tcgcggccgc gggagcgagg atacacctgc ccgaggtcgt
     1381 ctgttgcggc aggcccatgc tctccgaagg gctggtcgac gaggcgagag agaatgccag
     1441 gcgtaacctg gatctcctga tgcccctcgt ggagcgcggt gtcccgctgg taggcctcga
     1501 gccaagctgc atcctgaccc tgcgcgacga ttacaggaag cttctccccg acgacgagcg
     1561 cgtcgacaga ctggcccgtg caacccgtct cttcgaggag gcgatgctag atctcgatac
     1621 agagcttccg ctaagagaag gcaccccagt tctcctgcac gggcactgcc accagaaggc
     1681 gcttgtgggt acgggaccga cggagcggct gctcggcttc gccgccgatg tcgaggtcgt
     1741 ggactcaggt tgctgtggta tggccgggct cttcggttat gagaagggac actacgaggt
     1801 ctcgatgaag atgggagagc ggaggctatt ccctgcggtg cgcgaagcag gggagcgggt
     1861 cgtcgtcgca ccggggacga gctgccgcga gcagatcctg gacggaacac gacggcgggc
     1921 cctgcatcca gcagagtacc tggcttcact cctcgacggg ccatcctagc gaccgttggc
     1981 ggagctggag aaagcgctgt ggttcgcttt caggggcgag aggatgctcg tgctcgagga
     2041 cgcgcctgtt cgtgtgcctc tggccgatag tctggatgag ctcggtttgg atgttctttt
     2101 ctgtctggag attggagagc tcggagggca cgcttgctgg gccgtcgagg taagggctga
     2161 cgcccgaccg cccgagggga tggtcttcga ggatctcagg gccctgttct acagggtgga
     2221 cgaggaattc ttcaggatgg ctggcagggc caagcagatc gtcggctggt acgcgacgca
     2281 ccggttctgc gggcgttgcg gcggagaaac gaagcccgcg ccggtggaat tcgcgatgag
     2341 gtgttcgcgc tgcgggatga tgcactttcc gcgcctcagc cctgctgcca tcgtcctggt
     2401 caggagaggg gataggatac tgctggcaca ctcgcccgga tttccgaagg gactctacag
     2461 cgtgctcgcg ggtttcgtcg agccaggaga gtccatagag gaggccatcg tccgcgaggt
     2521 gcgtgaggag gtagggatcg aggtcactaa cgtcaggtac ttcgggagcc agccgtggcc
     2581 tttcccgaac tcgctcatga tcggctttac cgcagattac atggatggag agctgaaccc
     2641 acaaccaggt gagatcgaag acgccggctg gtacaccgca gaagacctcc cacaactgcc
     2701 gcccaggata agcatcgccc gcgccatgat agacgacttc gtaagacaac agggcctcga
     2761 cccggaaggg ctaaagatac ccggataggc tgagattgca cctcttgtat cataaggggg
     2821 cgaacagaac cggaagaaat ctcggtgaaa ggaacgttga cgcggaggca gttcatatgg
     2881 ctcgccggtg tggggggata gccctggtgg gcataaccta tgtcctctgg cgaaggcggt
     2941 ctccggttct attgcacgtt tactgttcgg attccctaaa ccagcgtcct acgtcagacc
     3001 tatcttcgta gaacacggcc ggttcgccga ccggagcgtg caccgtctcg taggctccct
     3061 tgtccagaga gccgtacttc cgacccgacc aaaggtgcac ccacctaccc gctggaaggt
     3121 agacctcgac tatctcctcg ccggggtcga ggaccggtgc caccatcagg ttacggccga
     3181 ccatgaactg atagtccagg ccaagcacct ctgggtcgtc gggatagtgc acgaaagggt
     3241 ggcggatgac ggggagccca gtctcggatg cttccttcac cagttcgatc ctgtaaggct
     3301 tccatgcggt gtagaccttg gcgaagcggg aaaagtgccg gagggtctcc tcatccgagt
     3361 agatctggtg gttcaccttg ggtctgttgc cttcgtgagt gcggaagacg gtcgtaaagg
     3421 cgccgagctc gatccagcgc atgagcaact cgttagagcg atgatatttc agcaggaagt
     3481 tgtctatggc cgtgtagcct ccgatgtccg agtgttcgag gctatagcct gagagcccac
     3541 tggagagcag gcccgtgacg gcgctcttga taccgtcatg ttcatcccaa tcaacgagtt
     3601 gatcacccag ccagaacagt gtgctctcgc cgggactacg tgtataaccg gagcggttga
     3661 agaagacgat atcctctcct cgccccgcct cccggatagc ctcgcggttg acctcggccc
     3721 actcctcggc gtagcggttg tggtagctct tcgggtccgc gtcggagaac agaacggcat
     3781 cgtaggggag tccctcaccg aagtccgcca tccatcccga ggcaccgttg cctatgagtt
     3841 cctccttgat aacgtccttg atccaatccc gcgccgcagg gttcgtcagg tcaacgaagg
     3901 ccgcccggaa gtcggtagtc ttgaccctgt aaggttcgcc ctcccgattc tttaccaagt
     3961 aaccttttct cgccgcctcc tcaaagaggt tgcggcggta gttcggcttc tcctgctcca
     4021 cgtcgtcggc gagccacggg ccgatgtagg tcatgagcct cacgtcgtcc ctctctagct
     4081 gctcccggag cctatcccag ccgggatagt gaacttcgtc gagctcccaa ttccaccaca
     4141 gctgcatccc gaagtcagtc tctcgctgcc ccacccaatc ctgcagccag agtgcagcga
     4201 cgggtgtgcc gagagctttg agtttgtcgt attcatcgag gaccttatcc gtgccgccct
     4261 gcaagccgac gaccgcgcca ctgaggatcc actctggcaa caggcgcatc ctgcccgagt
     4321 actctgtgta gagctctatt agttcgacag gggtctctcc gctcaggatc tggcctgtca
     4381 tttgggagga gaacacctcg acttggaccc tgttcctctc ccgcagatcg aaggaagagt
     4441 actcgtagtt ctcaagaaac acggagcgca tctggcttgt tatgtagtgc ggtacgctgg
     4501 caccgctggt ataggggctc ccgccggcgt cggccttcca atccgccgcc agcgtgattg
     4561 gctgcttgcc cctgcctata ccctgctcct ggatgaagat gggcaccttg tggcccttca
     4621 tgtcgaagta cgtgtactgc gtcccgaaac cgaagaagcg ctcctcaggc gaggaggcgt
     4681 aggtgagata gacgcggttg tagggctctt ccacctcgat ctcgaagcgc agccgttcct
     4741 cgctcacggg caagaaggtg agcgtatagt ccacgccttc tgaatcgcct cctcgtgtga
     4801 gccgtccacc gacgaccagg atagactggt gcttctcgat gtagtcgatg gtttgattcg
     4861 agtgtaggct ctctatctca tcatcgataa agaagtgccc gcttgactct ctgaccgtct
     4921 cctcgccctc ggcagcggcc aagaagctcg tcccgggggt gctctgccac agtatcctgt
     4981 caggcctgga gctatgagta acgctgagaa ctacgtctga aaggcttgga ccagtctcca
     5041 gcacgacggt aaacgcgccg atccggtgcg gcccccggga gacgtcgcgc tccactccgg
     5101 caatagcgcc ggagatacga gccggccttc tccagagcgc gtaggcacca cccagtagag
     5161 ccatcccagc cacaccaata ccggctagcc aaataaactt cctccgtgtc atcaatctcc
     5221 tctccgcggt ctctttcggc ccgtctcgcg ccccgcacag gatgtacttt cgactcaatc
     5281 ccacattctc ctgcatcata cagtcaatgt agcccaagcc agaagatcca agtctccacc
     5341 tcctacttga ggttagtcct tggagtcgct tagactagag ccgcctgctt gcccgaccta
     5401 agtgctagat gctgaatgtg ggcatgggga gctcaactct cgaaaatgcg tgcttccact
     5461 gtcgtgcctt ccggaagtcg acttatccac tgcacactag ctcatttggc cggccttggg
     5521 agtcaattgc accattaaag acccgatccc tcctcactac tgaaggcaag aacagcccgg
     5581 tgccacggca cactccgccg ttcaacgttg atcgcccacg tcgccatcaa actgttgtcg
     5641 aaagtttgct cttcacgtcg gagcgtcctc tgcttgtacc tgcgatgtgt tcatatccga
     5701 agattcggca ccggccgact caaccggatg ttctccttcc ccagttcatg gtcggtcccg
     5761 agctaatgtt cgcgcccgtg ctcgatcccg gcgaagacag cgtgggggtc tacctcccgg
     5821 cgggcagatg ggtccacttc tggacaggaa agagattcgg ttcgccacag cgcggcgtca
     5881 acgagacggt cccggctccg atcggagaac ccgccgtctt ctacaaagaa ggttctgcgg
     5941 aagggacacg tttcaggaaa tgctcgaacg ccggggactg ctatgacgct gcagaggtag
     6001 aatgaagtcg ttcttttcgt gcgagggaca atcatgaagc gggagaacgt aaccagcggc
     6061 acggtatggg agagcagggt cggctactca cgggcggtcc ggcgcggtcg tttcgtgtac
     6121 gtctcgggga ccacggccac ggacgggagc gggggatacg tcggtgtagg cgacccctac
     6181 gcgcagaccg tacaggccat aaacaacatc agggtggcgc tccaggccgt cggcgcggat
     6241 ctctctgacg tggtcaggac caggatctac gtggtcaaca tcgaggactg ggagaagatc
     6301 gggtcagccc acggcgagtg cttcggcaac gtccgtcccg ccacgagcat ggtcgaggtc
     6361 agccgcctca ttgcacccga gatcctggtc gagatcgaag ccgacgccct gatcgacgaa
     6421 tcgtaggagc ggtccatgta tggccgaacc aaatacggat gcgcgtgccc gaactatcta
     6481 tgtaggggcg gttcgcgaac cgcctgagga cgaaccgccc tcgtacaaac cgcccgacga
     6541 cgaaccgtac gggcgggttt gatgtacggg cgggtttcaa acccgcccct acgacgtttc
     6601 atattgccca accacgcatc gccattaccc gatctctgag gcgcggcctg cggcgtcctc
     6661 cagggcctgc agggcggagg cctgatccat ccccgtgctc ctgagcaggt cgacggcggc
     6721 ggagcggacc tggccgacga ggacgctgat cccgagctcg tggcgatcct ccaggatctc
     6781 ggtggcggtc ctggcggctt cgagcgccca atggcgcgcc tcctcgggtc ccccgccatc
     6841 ctccagaaac gcggccaggg cccttacgga ccgcgagagg tcgagtacgg cctcgggcaa
     6901 ctgcggggct attctgtctc ctcgtctgat ggcgttgacg acgccacggg ccaagacgcg
     6961 ggtgtttatg acggccagct ctatccttat t
//
LOCUS       DATMVF010000095         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_422987, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000095 DATMVF010000000
VERSION     DATMVF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422987"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..272)
                     /locus_tag="VN178_04390"
     CDS             complement(39..272)
                     /locus_tag="VN178_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80242.1"
                     /translation="MELDRTVPPVSHFYRGGTSEAKRILERFIEKGLDAYVEHRNQPQ
                     TDYVSHMSKYLHYGHVSPVYVAQNHSALIRQSA"
     gene            complement(319..819)
                     /locus_tag="VN178_04395"
     CDS             complement(319..819)
                     /locus_tag="VN178_04395"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HWS80243.1"
                     /translation="MYWKQEAQRASYNQTPEYAVQRANELEQPLLVVFGLTADYPEAN
                     LRQYAFMLGGLKDAGEALEERGIQLAIYSGSPDEVALRSGENASMLVCVMSQLRLQKE
                     WRERLAKEAPVSSCRWRPKSWCRSSWPQPSANTLPEPCGPGFESSSTTFWSSSSPPHS
                     ISRPWM"
     gene            1091..1516
                     /locus_tag="VN178_04400"
     CDS             1091..1516
                     /locus_tag="VN178_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 family protein"
                     /protein_id="HWS80244.1"
                     /translation="MSYPYSGIDLREHPHAYEIGRGEQGVFHAEPYKSELLPLWSFKD
                     AEAAKRSADDIYARFLEYRVEGDFVGMDMARKYLQMGYTRSRRYAKYGGGNKSKPLQR
                     PDLQKSRAAEIFYKKWRDVAEDEEYLRLKKEHRRRGSSG"
     gene            1793..2359
                     /locus_tag="VN178_04405"
     CDS             1793..2359
                     /locus_tag="VN178_04405"
                     /inference="COORDINATES: protein motif:HMM:NF039561.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5872 domain-containing protein"
                     /protein_id="HWS80245.1"
                     /translation="MTKKSREHKYTDPDLREEIKDEIKQSDKGGRPGQWSARKSQLLA
                     REYERRGGGYTGEKDEGQKNLEKWTEEDWQTQEGETRARSGEETARYLPKEAWEKMSD
                     EETRQTERRKREGSKRSEQYVANTKEARRARRGSQAPPINDYDNLSIDEVEKKVRGIS
                     RQEIQDVRDYEKRHKNRKTLIESLDRSL"
     gene            2425..3039
                     /locus_tag="VN178_04410"
     CDS             2425..3039
                     /locus_tag="VN178_04410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWS80246.1"
                     /translation="MRDGTEDRIFLTFDDGPDPIWTPRILEVLHHSRARATFFVISPL
                     ALRFSHLVRRMIHAGHGVELHCAEHIRHTELTHAEVEADTRDGLEDLHALGVHPRLWR
                     PPWGVITPQTQAVAKDLGLELVSWTADTHDWRGDTASQMFTNVRPDLQPGAVVLMHDG
                     LGPGVRRSGCEETVALIEKVVKQARSVGCEPCPLGDPYARLSAG"
     gene            3054..3605
                     /locus_tag="VN178_04415"
     CDS             3054..3605
                     /locus_tag="VN178_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS80247.1"
                     /translation="MRTDERIGNTIQARTATVIEFVLSQIASGAAERDAHPTFPEGPF
                     RALAAAGILAMPAPEPGTGSRRRASFAEEWSMLRAVARVDASVGRILDGHLNGVERVS
                     VLAPEPLRSRELAGVAAGRLRLGVWGADPIPGEGTPARLVATGGGYSLQGVKTFCSGA
                     TGLDRALVAVRAPAGGSRPGPRC"
     gene            3665..>4119
                     /locus_tag="VN178_04420"
     CDS             3665..>4119
                     /locus_tag="VN178_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80248.1"
                     /translation="MRSSESHRVVFEGAPVLAMLGEPGELVREPYFGRDAIRTAVTWA
                     GVADSAVEGALDILATKSQGRPPDDLVSLAAGRMLASRGTIDRWLEHAASFVDTNPEA
                     STAHFSTQLRESIAAAARDILDVAARAVGSRPFVTASDLDRARRDLELFL"
BASE COUNT          821 a         1257 c         1324 g          717 t
ORIGIN      
        1 tcgcctgttt caagacgaag ttctaaagaa tttaccttct acgcactctg gcggatcaag
       61 gccgagtgat tctgcgccac gtagaccggc gagacgtgac cgtagtgcag gtacttgctc
      121 atgtgcgaga cgtagtcggt ctgcggctgg ttgcggtgct ccacgtaggc gtcgagcccc
      181 ttctcgatga accgctccag gatacgcttc gcctctgagg tcccgcccct gtagaagtgg
      241 ctcaccggag gaacggtccg gtcgagctcc atcccgtcga ggatcgcgcc gagtcggaga
      301 ggtcgaggcc gctcgtttct acatccaggg acggcttatc gagtgcggtg ggctcgagct
      361 cgaccagaaa gtcgtcgagc tgctctcgaa tcctgggccg cagggttcgg gcagcgtgtt
      421 cgcgcttggc tgaggccagc tcgaccggca ccacgacttc ggtctccacc tgcacgacga
      481 gacaggagcc tccttcgcca gcctctcgcg ccattctttc tgcaagcgca gttggctcat
      541 aacgcagacg agcatcgagg cgttctcgcc ggacctgagg gccacctcgt ctggcgaacc
      601 cgaatagata gcgagctgga tccctcgttc ttccagggcc tccccggcat ccttcaggcc
      661 cccgagcatg aacgcgtatt gcctgaggtt cgcctcgggg tagtcagccg tcaacccgaa
      721 gaccacgagg agcggctgtt cgagttcgtt ggccctctgc accgcgtact ccggggtttg
      781 gttgtaactg gcgcgctggg cttcctgctt ccagtacaga acgtagttac cgtcttagac
      841 atccttgtcg atgagttgac tgatacgctc ttcctggata cttctcattc ccgatctcca
      901 ctccaggacg ccgcaagccc tcatctccgg acgtaccacc gctagacgaa ccatgctgag
      961 tgagataaca ctcatcatgg tcggttagca atccaagaca gtctgcccgg agacagtact
     1021 tcggagagaa aagagctctc cgttgatgct atcgaagtcc gggtcaggtt ttctcgtgta
     1081 aagttcgctc gtgagctatc cgtacagcgg catcgatttg cgcgaacatc cgcatgcata
     1141 cgagataggt cgtggggagc agggcgtctt ccacgccgaa ccctacaaga gcgagctgtt
     1201 gccgctgtgg agtttcaagg acgcagaggc agcaaagagg tcggccgacg acatctacgc
     1261 gcggtttctc gaatacaggg tagagggtga cttcgtcggg atggacatgg cgagaaagta
     1321 cctgcagatg ggttacaccc ggtcgcggcg gtacgcaaag tacgggggtg gcaacaagag
     1381 caagcccctg cagagaccgg accttcagaa gagccgggca gcggagatct tctacaagaa
     1441 gtggcgcgac gtcgctgagg acgaagagta cctaagactc aaaaaagagc accggcgacg
     1501 cggctcgtca gggtgagaga ccaagctcca cgacactccc gtacccgctg tgcatcaact
     1561 caagcaaggc tcgtgaaacg tcaagatgag cgtcgcctca gagcgaagtt ctagaaccgc
     1621 cgcgcagaga acaagatctg tatggcaaat taccgaggtc tcgcccggca gcgattaacg
     1681 tctacgggtt ggcaatccga tggtgagcga cacgccaggg aatgctgtga atgagcctga
     1741 ggctattcat actcgttcga aaaactagca ggtgccaggt caggagacgt ccatgacaaa
     1801 gaaatcgaga gagcataagt acacggaccc ggatcttagg gaagagatca aagatgagat
     1861 caagcaatcc gacaagggtg gcagacccgg acagtggagc gcccgcaagt cccagctgct
     1921 cgcccgggag tatgagcggc ggggtggcgg ttacaccggc gagaaggatg agggtcagaa
     1981 aaacctcgag aagtggaccg aggaggactg gcagacccag gagggggaga cgcgagcccg
     2041 gagcggcgag gagacggctc gctaccttcc gaaagaggct tgggagaaga tgagcgacga
     2101 agagacgcgc cagacagaga gaaggaagcg cgaaggctcc aagcggagcg aacagtacgt
     2161 cgccaatacg aaggaggcgc ggcgggcccg cagagggtcg caggccccgc cgatcaacga
     2221 ctacgacaat ctgagcatcg acgaagtgga gaagaaggtg agagggatct ccaggcagga
     2281 gatccaggac gtacgcgatt acgagaagcg gcacaagaac cgcaagaccc ttatcgagtc
     2341 gctggaccgc agcctctagt cggcagaggt ctgggtcgag aggttgtttg agctcacgga
     2401 tcaagacgag tcgggggtgg ccggttgagg gacgggaccg aagatcgtat cttcctgacc
     2461 ttcgacgacg ggcccgaccc tatctggacg cccaggatcc tcgaggtgtt gcaccactct
     2521 cgcgcaaggg cgacattctt cgtaatctcg cccttggcgt tgcgtttttc tcaccttgtt
     2581 cgccgcatga tccatgccgg tcacggcgtg gagttgcact gcgcggagca catccggcat
     2641 acggagctca cccacgccga ggtcgaggct gacacacgcg atggcctgga agacctccat
     2701 gctctaggtg tgcacccgag gctgtggcgt ccaccatggg gcgttatcac tccgcagact
     2761 caggccgtgg cgaaggacct cgggctggag ctcgtctctt ggaccgccga tacccacgac
     2821 tggcgcggag ataccgcatc gcagatgttt acaaatgtac gaccggatct tcaacccgga
     2881 gccgtcgttc tcatgcacga cgggctcggt cctggtgtca ggcgctcggg ctgcgaagag
     2941 accgtcgcct tgatcgaaaa agtcgtcaag caggcgcgct ctgttggctg cgaaccgtgc
     3001 ccacttggcg atccctatgc taggctttcg gccggatgaa ggcaaacgaa cctttgagaa
     3061 cagacgagag gatcggtaac acgatccagg cgcggacggc gacggtcatc gagttcgtcc
     3121 tctcccagat cgcctccggc gccgccgaac gcgacgcgca cccaaccttt cccgaaggtc
     3181 ctttcagagc actcgcggcg gcaggaatcc tcgctatgcc tgcgcccgag cccggcaccg
     3241 gaagcaggcg ccgcgcatcc ttcgccgagg agtggagcat gctgcgcgcc gtcgctcggg
     3301 tcgacgcttc ggtcggcagg atcctcgacg gccacctcaa tggcgtcgag cgcgtctcgg
     3361 tgttggctcc cgaaccgttg cgctccaggg aactcgcggg cgtcgccgcc ggacgcctcc
     3421 gactcggggt gtggggtgcc gaccccatac ccggagaggg gacaccggcc cggctcgtcg
     3481 cgaccggggg cggctactct ctccagggcg tcaagacgtt ctgcagcggc gcgacgggac
     3541 tcgaccgcgc gctcgtggcc gtccgcgcgc cggcgggggg gagccgcccg ggcccccgct
     3601 gctagcttac gttgacctct ccgagggtgt agagatcgat gacggttggt tcaaggcgtc
     3661 ggggatgcgc tcttcggaga gccaccgcgt cgtcttcgag ggagccccgg tgctcgcgat
     3721 gctcggcgag ccgggggagc tcgtgcgcga gccgtacttc ggccgcgacg cgatccggac
     3781 ggccgtcacc tgggccggcg tcgccgactc tgcggtggag ggagccctgg acatcctcgc
     3841 cacgaagagc cagggcaggc cgcccgacga ccttgtctcc ctggcggcgg gccggatgct
     3901 cgcctcgcgt ggcacgatag accgctggct cgagcacgcc gcctcgttcg tagacacgaa
     3961 ccccgaagcc tcgacggccc acttctcgac ccagcttcgc gagtccatcg ccgccgccgc
     4021 ccgcgacatc ctcgacgtgg cggcccgcgc cgttggctct cgccccttcg tgacagcctc
     4081 ggatctggat cgtgcccggc gggatctcga gctctttct
//
LOCUS       DATMVF010000096         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_288374, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000096 DATMVF010000000
VERSION     DATMVF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288374"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VN178_04425"
     CDS             <1..1334
                     /locus_tag="VN178_04425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWS80249.1"
                     /translation="MGCELGAEGVPENVEQIAGGQDESIFQPEWSPEGTLHFVSDRTG
                     WWNLYRRRDGRVEPLCEREAEFGLPQWAFGMSTYAFVSPGRIVCSYTEHGSSHLAILD
                     TESGELEPLETPYSSIAYLRTDSSGGVVFRGSSPTKAACIVRLDPSTVQHEVLRRSGD
                     LEIDNGYLSVPEPVEYPTEDGRTAHAFFYPPKNRDYAAPDGELPPLLVMSHGGPTAAS
                     TTSFDPGIQYWTSRGIAVLDVNYGGSTGYGREYRRHLEGAWGVVDVDDCANGALYLAE
                     RGLVDGERLIITGGSAGGYTTLCALAFRDTFAAGASHFGISDVEAMAMDTHKFESRYL
                     DSLIGPYPGRKDLYLERSPIHSAEQLSCPVIFFQGLEDEVVPPDQAEKMFAALREKGL
                     PVSYVPFEGEQHGFRRAENIKRALDGELYFYSRVLGFELADPVEPVPIENL"
     gene            1474..2289
                     /locus_tag="VN178_04430"
     CDS             1474..2289
                     /locus_tag="VN178_04430"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS80250.1"
                     /translation="MGEWRDEWREANRASWDERVPIHVSGEFYDVASFKGGQERLQPF
                     EIAEVGDVSGKELLHLQCHFGIDTLSWARRGASVTGLDFSAPAIEAARGLASEMGLEA
                     TFVHSDVYEAVGATGGRTFDIVYTGRGAIIWLPDIGRWAEVVADLLKPGGFLYLTEFH
                     PFTDVFGDDDLSVEHDYFQDENPRVYDEPGTYADFDAETSHNLTYEWNHTLGNVVSAV
                     SATGLNVELLHEYDYTMSPRWPILEKSGFDTYRLPEGTPKIPLMYSLRARKGS"
     gene            complement(2325..>2890)
                     /locus_tag="VN178_04435"
     CDS             complement(2325..>2890)
                     /locus_tag="VN178_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWS80251.1"
                     /translation="VLAGLALILFAAGLVFLDVRRAGEETAAPPPKRDVTMKLSIPSM
                     KHVDNVPVYTAAAHNQPALRNGTLHLAGTGFPWQREANVYIAGHRLGYPNTESFLVFW
                     DLNRLRMGRRVVLTDSEGERYIYRVYDRFVLGPNDASATKPVAGRNVVSLQTCTLPNY
                     TDRLIVRAELVKILQGPAPRGAPNKPA"
BASE COUNT          539 a          926 c          919 g          506 t
ORIGIN      
        1 taatgggctg cgaactcggc gccgaaggcg tccccgagaa cgtcgagcag atcgccggcg
       61 gacaggacga atctatcttt cagccggagt ggtcccccga aggcacgctg cacttcgtct
      121 cggaccggac gggctggtgg aacctctacc gccggcgaga tggacgcgtc gagccgctct
      181 gcgagaggga agcggagttc ggcctgccac agtgggcatt cggcatgtct acctacgcct
      241 tcgtctcgcc gggacgcatc gtctgctcgt acacggagca cgggagctcg cacctcgcca
      301 tcctcgacac ggagagcggc gagctcgagc cgttggagac gccgtactcg agcatcgcat
      361 acttgcgtac tgatagctcc ggcggtgtag tcttccgcgg cagctcgccc acgaaggcgg
      421 cgtgcatagt gaggttggac ccgtctaccg tgcagcacga ggtcctgcgg cgctcgggcg
      481 acctggagat agataacggc tacctctccg tccccgagcc cgtcgagtat cccacagagg
      541 acggccggac ggcgcacgcc ttcttctacc cgccgaaaaa cagggactac gccgcccctg
      601 atggtgaact ccccccgctc ctcgtaatga gtcacggggg tccgacggcg gcgtcgacca
      661 cgtcatttga cccagggatc caatactgga ccagccgagg tatcgccgtg ctcgacgtga
      721 actacggcgg aagcacgggc tacggtcggg aataccgtcg tcacctggaa ggggcgtggg
      781 gtgtcgtcga cgtcgacgac tgcgccaacg gagcactcta ccttgccgag cgtggactcg
      841 tcgacggcga acggctgatt atcaccggcg gtagcgctgg cggctacacg acgctctgcg
      901 cgctcgcgtt cagggacacg ttcgccgccg gagcgagcca cttcggcatc agcgatgtcg
      961 aggccatggc aatggatacc cacaagttcg aatcgcgcta cctggatagc ctgatcggac
     1021 cctacccggg gcgcaaggac ctctatctgg agcgctcacc gatccactct gccgaacagc
     1081 tctcctgccc cgtaatcttc ttccagggcc tcgaagacga ggtcgtgccc ccggaccagg
     1141 ccgagaagat gttcgcggcc ctcagggaga aggggctccc tgtctcctac gtacccttcg
     1201 agggcgagca acacggcttc aggcgggccg agaacatcaa gcgggccctg gacggcgagc
     1261 tgtatttcta ctcgcgcgtc ctgggcttcg agcttgccga cccggtcgaa ccggtaccga
     1321 tagagaacct gtgacagcgc cgtgcagacg gccctctgca cggcgcgcta tcagccatta
     1381 gcattcagcc atcagcaaga acaagtagct gatggctgac ggcgcggttt gtggaaggtc
     1441 gatccttcca caagccgcta tgagaggaga gcgatgggtg aatggcgcga tgagtggcgg
     1501 gaggcgaaca gggccagctg ggacgagcgt gtccctatcc acgtctcggg ggagttctac
     1561 gacgtggcct ctttcaaggg tggccaggag cgcctgcaac cgttcgagat agccgaggtg
     1621 ggcgacgtga gtggcaagga gttgttgcac ctccagtgcc acttcgggat cgacaccctg
     1681 agctgggccc gccgcggcgc cagcgttacg ggcctggact tctcggcccc ggccatagag
     1741 gcggcccgcg ggctggcctc ggaaatgggg ctcgaagcca cgttcgtgca ctcggacgtc
     1801 tacgaggccg tgggcgccac ggggggccgc accttcgaca tcgtgtacac gggccgaggc
     1861 gcgatcatct ggctccccga catcggaagg tgggcggagg tggtcgcgga tctcctgaaa
     1921 cccggcggct tcttgtacct caccgagttc caccctttta cggacgtctt cggggacgat
     1981 gacctgagcg tggagcacga ctatttccag gacgagaacc ccagggtata cgacgagcca
     2041 ggtacctacg ccgacttcga cgccgagaca tcgcacaatc ttacctacga atggaaccac
     2101 accctcggca acgtagtaag cgcggtgagc gccacaggcc ttaacgtcga actgctacat
     2161 gagtacgatt acacgatgtc tcccagatgg cccatcctgg agaagtcagg gttcgatacc
     2221 taccgtctgc cagagggcac gccaaagatc ccgctcatgt attcgctgag ggcgcgcaag
     2281 gggtcgtagg ggcgggtttc gaacccgccc acaacgcacc gcccctacgc cggtttgttc
     2341 ggcgcacccc gcggcgccgg gccttgcagg atttttacga gctcggcgcg cacgatcagg
     2401 cggtccgtgt agttcggtag cgtgcaggtc tggaggctta ctacgttcct ccccgctacg
     2461 ggcttggtag ccgaggcgtc gtttgggcca agcacgaagc ggtcgtagac cctgtagatg
     2521 tatctctcac cctccgagtc cgtgagcaca accctccgtc ccatcctcaa cctgttcaga
     2581 tcccagaaca ccagaaagct ctcggtgttc gggtagccaa gcctgtgacc cgcgatgtag
     2641 acgttcgcct cccgctgcca gggaaagccc gttccagcga ggtgcagcgt cccgttgcgc
     2701 aaagccggct ggttgtgcgc ggcggcggtg tagacaggca cgttatccac gtgcttcatg
     2761 ctcgggatgg acagtttcat cgtcacgtcc ctcttcggcg gcggggcggc ggtctcttct
     2821 ccagccctcc tcacatcgag aaaaacgagc cctgccgcga acaggatcag ggccagaccg
     2881 gccagcacga
//
LOCUS       DATMVF010000097         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_475076, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000097 DATMVF010000000
VERSION     DATMVF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475076"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VN178_04440"
     CDS             <1..1149
                     /locus_tag="VN178_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWS80252.1"
                     /translation="DLDDLRRRIAATQWPEKETVADESQGVQLATIQELARYWATDYD
                     WRKAEEKLKALPQFITEIDGLDIHFIHVRSNHESALPLIVNHGWPGSIIEQLKIIDPL
                     VDPAAHGGSAEDAFDVVIPSMPGYGFSGKPTSTGWGPERMARAWAELMKRLGYTRYVA
                     QGGDWGAFVVDQMGLQEPEGLLAIHSNMPATVPADVDEALQAGDPPPSGLSTEERRAY
                     EQLERTFKQVEYANYMAARPQTLYGIADSPVGLAAWLLDHNDADGQPAAAVTKALKRT
                     TSATGELTRDEILDNITLYWLTNTGISASRLYWEYKGGFFNAKGVTIPVAVSVFPGEQ
                     YEAPRSWAEKAYPDLIYFNEVDKGGHFAAWEEPQLFSEEVRAAFRSLR"
     gene            1246..3060
                     /locus_tag="VN178_04445"
     CDS             1246..3060
                     /locus_tag="VN178_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS80253.1"
                     /translation="MTAENTADPSAPDTVVLIHGLWVTPRSWEHWIEHYEGRGYRVLA
                     PAYPGLEVEVEALNEDPSPIEALTIPGVVEHLEGIVGELENPPIIMGHSAGGLFTQIL
                     LDHGYGAAGVVIDSAPAEGVRVTPIAQIRALFPVLKNPANRHRAVGFTKEQFHNAFAN
                     TLSREDSDEVYERYHIPAPGSFVWAGPLANFTPGHQDTYVNFRNEERAPLLFIAGSED
                     NIMPPAVNQSNVKHYRHAKTVTDYKEFAGRSHYTVGQDGWEEVADYALEWANEHATTR
                     PARKEILMSSSPTHLSTPEGPGSVSGAPNLPAGFTDTFTSRFIEANGLRQHAVIGGEG
                     PPLLMVHGWPENWYAWRLLMPQLARDFQVIAVDQRGIGLTDKPQDGYDTGTLAGDLVA
                     LMDELGHERFAVVGHDTGYFIGYALAADHPDRVDRMALAEVPGPLGVNPSPPLFGLPE
                     PINNKLWHLAFNRVNDEMTEQLVRGREDIFFGYEFAIQGGQKGLPDEVQRYYFDLYSD
                     PDVLRGSFGLYRALDTTLAQNAERKNRPLTMPVLAIGGAESWGEEAANGMKPAADDVQ
                     TVVIPDTGHWVAEQAPDQMLEALTEFLAPYREDGGGLR"
     gene            3057..>3307
                     /locus_tag="VN178_04450"
     CDS             3057..>3307
                     /locus_tag="VN178_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80254.1"
                     /translation="MSLFQSLFPAHREVGTDFLPSEGRLPPFEGATGWLGSEPLTPEG
                     LRGRVVAVQFWTYTCVNWLRTLPYVHEWAHKYHDHGLIV"
BASE COUNT          641 a         1105 c         1030 g          531 t
ORIGIN      
        1 gatctcgacg acctccgtcg ccgcatcgcg gccacgcagt ggcccgaaaa ggagaccgtc
       61 gcagatgagt cccagggcgt gcagctcgcc acgatccagg aactcgcgcg ctattgggcc
      121 acagattacg attggcgcaa ggccgaggag aaactgaaag ccctaccgca gttcatcacc
      181 gagatcgacg ggctcgacat ccacttcatc cacgttcgtt cgaaccacga aagcgcgttg
      241 ccgctcatag tcaaccacgg atggcccggc tcgatcatcg agcagctgaa gatcatcgat
      301 ccacttgtcg atcccgcggc acacggcggg agcgcggagg acgcgttcga cgtcgtcatc
      361 ccgtcgatgc cgggctacgg gttttcgggc aagccgacga gcaccggctg gggccccgag
      421 cgcatggcac gtgcctgggc agaactgatg aagcgcctcg gctacactcg ctacgtcgcc
      481 cagggcggcg attggggtgc gtttgtcgtc gaccagatgg gcttgcagga acctgaggga
      541 ttgctcgcca tccactccaa catgcctgct accgtaccgg ctgacgtcga cgaggctctc
      601 caggccggcg acccgccgcc atccggtctc tcgaccgagg aacggcgcgc ctacgagcag
      661 ctggagagaa ctttcaagca ggtcgagtac gccaactaca tggcggcgcg cccgcaaacc
      721 ttgtacggca ttgcggattc acccgtcggc ctggcagcct ggcttctcga ccacaatgat
      781 gccgacggcc agccggccgc ggcggtcaca aaggccctga agcggaccac gagcgccacg
      841 ggcgaactga cgcgtgacga gatcctcgac aacatcacgc tttattggct gacgaacacg
      901 gggatctccg cgtcacgtct ctactgggaa tacaagggtg gcttcttcaa cgccaagggc
      961 gtcaccattc cggtggcggt gagcgtcttt cccggcgagc aatacgaggc cccgcgcagc
     1021 tgggccgaga aggcctaccc cgacctcatc tatttcaacg aggtcgacaa gggtggccac
     1081 ttcgcggcct gggaagagcc tcagctgttc tccgaggagg tccgtgcggc gtttagatcc
     1141 ctacgctagt tgcgaacgca cacaaaagat gggtaatgga aggagagtaa tggaaggagg
     1201 aacttatatc tacttcgagg gaatagcgac gctaggagga tccccgtgac tgccgagaat
     1261 accgccgacc cgagcgcccc ggacaccgtc gtcctgattc acggcctctg ggtgaccccc
     1321 cgcagctggg agcactggat cgagcactac gagggccgcg gctatcgcgt gctcgctccg
     1381 gcctaccccg gcctagaggt agaggtagag gcgctcaacg aggacccctc cccgatcgaa
     1441 gctttgacta tccctggggt cgtcgagcac ctagagggca tcgtaggtga gctagagaac
     1501 cccccgatca tcatgggcca ctcggcagga ggcctcttta cccagatcct cctggaccac
     1561 ggttacggcg cggcgggggt agttatagac tccgcgccag ccgagggcgt cagggtaacc
     1621 ccgatcgcac agatccgcgc gctctttccg gttctgaaga accccgccaa ccgacacagg
     1681 gctgtcgggt tcaccaaaga gcagttccac aacgccttcg ccaacacttt gagccgggag
     1741 gactcagacg aggtctacga gcgctaccac atccccgctc cgggcagctt cgtgtgggcc
     1801 gggcccctcg ccaacttcac cccgggtcac caggacacct acgtgaactt caggaacgaa
     1861 gagcgggcgc cgttgctgtt catagcgggg agcgaggaca acatcatgcc tccagcggtc
     1921 aaccagtcca acgtcaagca ctaccgccac gccaagaccg ttacagacta caaggagttc
     1981 gctggacgct cgcactacac cgtcggacag gatggatggg aggaggtcgc tgactacgcg
     2041 ctcgagtggg ccaatgagca cgccaccacc cgcccggcca gaaaggagat cctcatgagc
     2101 tcatcgccca cccacctgtc taccccggaa ggccccggct ccgtttccgg tgcgccgaac
     2161 ctccccgcgg ggttcactga cacgttcacc agccggttca tcgaggccaa cggactgcgc
     2221 cagcacgcgg tcatcggagg cgaggggccg ccgctgctga tggtgcacgg ctggcccgag
     2281 aactggtacg cctggcgcct gctgatgcct cagctggccc gggacttcca ggtcatcgcg
     2341 gtcgaccagc gcggcatcgg gctgactgac aagccccagg atgggtacga caccggcacc
     2401 ctcgccggcg acctggtcgc gctgatggac gagctcggcc acgagcggtt cgccgtggtt
     2461 ggccacgaca ccggatactt catcggctac gcgctggccg cggatcaccc ggaccgggtc
     2521 gaccgcatgg ccctcgccga ggtccccggg ccccttgggg tgaacccctc gccgcccctg
     2581 ttcggactcc ccgagccgat caacaacaag ctctggcacc tcgccttcaa ccgcgtcaac
     2641 gacgagatga ccgaacagct cgtcaggggg cgagaggaca tcttcttcgg ctatgagttc
     2701 gccatccagg gcggccagaa ggggctgccc gacgaggtcc agcggtacta cttcgacctc
     2761 tactccgacc ccgacgtcct gcgcggcagt ttcgggttgt accgcgcgtt ggacaccacg
     2821 ctcgcacaga acgcagagcg caagaaccgg ccgctgacga tgcccgtcct ggcgatcggc
     2881 ggagcggaaa gctggggcga agaggccgca aacgggatga agcccgccgc ggacgacgtg
     2941 cagaccgtgg tcatccccga caccggccac tgggtcgccg agcaggcccc agatcagatg
     3001 ctggaggcgc tgacggagtt cttggccccg tatcgcgagg acggaggtgg cttgcgatga
     3061 gcctcttcca gagcctcttc cccgcgcatc gggaggtcgg aacggatttc ttgcccagtg
     3121 agggccgcct gcctcctttc gagggcgcca ccgggtggct gggctccgag cctttgacgc
     3181 ccgaggggct gcgcggacgc gtcgtcgcgg tgcagttctg gacctacacc tgcgtcaact
     3241 ggcttagaac gctgccttac gtgcatgagt gggcccacaa ataccacgat cacggcctga
     3301 tcgttat
//
LOCUS       DATMVF010000098        18053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_239215, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000098 DATMVF010000000
VERSION     DATMVF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18053
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239215"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VN178_04455"
     CDS             complement(<1..348)
                     /locus_tag="VN178_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80255.1"
                     /translation="MPNARWLTLFLLLYARDGDIRIDGLAERLGDLGFGAVTPEELHK
                     TMQQLKEEGLLEEDPLVPDGVPSGSGAEGYGLTLAGETYLHAWGDYLERYEKELGHFL
                     RLYRGGQDAHTSLA"
     gene            complement(617..949)
                     /locus_tag="VN178_04460"
     CDS             complement(617..949)
                     /locus_tag="VN178_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80256.1"
                     /translation="MDEESRKTHQELWEQYMEVERRELEQRRNGQLGRALGRALPGES
                     QEELQRIAEDDQRKAERGLVELRSGNEVWFKHIDEITRDDRQARIESENARAAWIQER
                     LRRQPRLQ"
     gene            1198..3783
                     /locus_tag="VN178_04465"
     CDS             1198..3783
                     /locus_tag="VN178_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HWS80257.1"
                     /translation="MADSSFTHLVVVGSSAGGIAALSGLVSSLPDEFDAPIVVAQHLD
                     PKRESHLQEILARKSPLPVKTVTEHEPLQAGVVYVVPANRHVNISDSEIELSSEQSSR
                     QPMPSINLLMETAAGVYGENLIAIVLSGTGTDGTEGARTVSQAGGTVIIQDPETAEFG
                     EMPRSLAPSTVDIVSSLEGIGPVLKSLISGEAAVEEISENKGRDLERFLEELHRNRGV
                     DFRSYKRPTIMRRLARRMAATNCQSLDEYYGYLEEHPEEYRQLIGAFLIKVTEFFRDP
                     ELFDYLREEVLPELIGEVREGEKQLRIWSAGSATGEEAYSLAILLSEVLGQEAGLFNV
                     RIFATDIDEGAVRFARRGIYPPSALGGLSEEQIRRYFVEEDGSYQVKKQIRGMIVFGE
                     HDLAQRSPFPEIDLVVSRNVLIYFSNELQRRALQLFAYSLRDGGYLVMGKAESASLLP
                     DLFASVDRHNKVYRRHGQRFLLPPTLPTSPIPRARLGRGSRDQGAPRRQAETPQLPSA
                     ALRDPGEDLLNQLPVGVAVVTRDYAIEAINVAAREMLAIPSVGVGQDFLHAMQEAPYA
                     EVRRTIDEAFREGRITQADEFAVEEAATGEPRYLRLTCHPRRAETEGQRVRRVLVAVE
                     DVTAEVRMRRLAEKNLRLEEANRELGQLNEELQAAHEESLVNTEEAQASAEEVETLNE
                     ELQATNEELETLNEELQATVEELNTTNDDLQARATELQDLAQSREEGRRRAEELVDQL
                     QDQRSRLEAILANISDAVLAVDTDRQTLFSNHVFEEVFGEHTGAEQGQLGFTEVLDER
                     GEKLPSEATPSSRAARGESFAMRFTVGGLDGVPRCFEARGRPIEGDGVAGGVLVIREA
                     TDGQD"
     gene            3794..5545
                     /locus_tag="VN178_04470"
     CDS             3794..5545
                     /locus_tag="VN178_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWS80258.1"
                     /translation="MRKPDWNTWAAGSRGVEVERLEQELAESRASERRARLEAAETRE
                     RFERLLDASRRFTRTLSDRRRQQQTSRQFLAAQHAIDGVLAESAGLEDAAAGVLRTLG
                     ENLGWQVAVFWVAGAETLRCIELWHRPDAAPDGFAMACRRTRFMRGEGLPGSAWAQNR
                     PVWTEDSPRDERFVGETVSSGYVLRGALAFPITGGGGPHGIIELLGSKGLHPDQELLY
                     TVGLIGGRLGHFADRRKAQKDLREAEERLRLATEAGRVGLLDWDVLTGESRCSGAMAE
                     IYGYPPGEFNLSYVEFLEKVHPDDRVRVRSTLDAAVAAGAPHELEFRIVRPTGDIRCV
                     HAKGRVHRDEKGVFARVLGVSLDVTERKQAEEERDRLHSLEVAARAEAAERNRISREL
                     HDRVAHSMGVAHQSLQLYEALAEKDPVRAHGKLRTAKEMMKTALEQTRNLSMELRRSE
                     TEHGLIPALQDLLEVAVPEGVRADLSTSGAESRLSDHQRGQLYLILREAVRNAVRHSG
                     CRTLRIGLDITSENVFGYVEDDGRGFEGNGGTRGGLGLRSIRERVALLQGTVEVYSSP
                     EGGARVQVQLPLRNGGG"
     gene            5547..6224
                     /locus_tag="VN178_04475"
     CDS             5547..6224
                     /locus_tag="VN178_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS80259.1"
                     /translation="MADSKIRVLLADDHAMFRQGIKEMLSTDEKIEVVGEAENGREAG
                     TLAEKLTPDVVLLDVEMPVMGAKGAMERMMKHSPPPRVVIVTMYDDPRLVRELIGLGA
                     VAYLVKSATMEELHAAVHTAANSPVGPDQNVVIVTPPKAFENPAEADGLSERELEVLL
                     MVARGMSNNQIAISLHLSEATVKRHLANIYPRIGVSSRGEAVRKALSNRWITARDITG
                     GGELREV"
     gene            complement(6231..6470)
                     /locus_tag="VN178_04480"
     CDS             complement(6231..6470)
                     /locus_tag="VN178_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80260.1"
                     /translation="MEPEKVNIGTTVRVRERHRVAERRGMVGKIADHYGGDGFIAVDV
                     RFSGGHRRLFWPEDLEEISSPPPWWRSLLGRAGAQ"
     gene            6659..7354
                     /locus_tag="VN178_04485"
     CDS             6659..7354
                     /locus_tag="VN178_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWS80261.1"
                     /translation="MDEDHTTLLSGVGLFASLSQEQLGRITHGIPAKSFGVGEHVFTP
                     TYRGGVFFLLLAGRVRIYRLEARQEVTICVLEAGEMFGEAAFTSRDGKGSYAQAIAVS
                     KVAFLNRSTFYRLIQRDPELGIRAIELLGERLSFYEQRIADLGLKKVPARLASLILQL
                     VEKEGIVTGKGRYRLSTHYTHEQLALMVGAKRVAVSRAMKGLREAGAVETGRRRIVVK
                     DAKALGRIADEVA"
     gene            7462..8334
                     /locus_tag="VN178_04490"
     CDS             7462..8334
                     /locus_tag="VN178_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HWS80262.1"
                     /translation="MVQRSEARSSGAPERPDRLYEGYVFDLDGTIYLGDGLLPGAKRL
                     ILKLRELGKRVVFVSNNPTKDPKMYAEKLGALGLKTPASEIVNTVVTMTEWLLRKRPE
                     ATVFPISEEPLKNSLSEAGIRMSEDPEEIDIVIASYDRSFDYRKLQIAFDAIWFYGRA
                     MLVTTNPDRYCPLPNGRGEPDAAAIVAAIEACTGSQCRVNVGKPDPIMLETVMDLIGL
                     DAKECVMSGDRLYTEIRMAKGAGIPSAIVLTGETTEEDLAGEPVENLPDYVLKRIDQL
                     VPRDLWDEFGWEDG"
     gene            8384..9955
                     /locus_tag="VN178_04495"
     CDS             8384..9955
                     /locus_tag="VN178_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWS80263.1"
                     /translation="MMADGPYLLGIDFGTGGARVGIFDPEGTPLVFSEREYALKHPRP
                     GWAEQDPDEWWSCLVAAVKGAMEESSISPEDIAGISIDAMSSTVLAIDERGRHLRPAI
                     MWMDVRSSEEADRIAGTNNNALKYNGYGAVSAEWGLPKALWIKENEPEVYRDARHICD
                     CGDWLIHRLTGEWTSSINIVSSKYYYDRDEGGYPESLYDAVDATDLLEKFPGDVLDLG
                     TLAGELRSEAAEELGLKTGTPVAEGGVDAYVGAIGLGVVEPGKMALITGSSHVMIGQT
                     ATPIHDPGFWGAYTDAMIPGQYTVEAGQASTGSIVAWFKNQFAGDAAAEARERGIDTY
                     DVLTELARDVPIGSDGLIVLDYFQGNRSPYTDPLARGMMWGLTLSHTPGHVFRAIIEG
                     ICYGTENILRTMRGQDFEPKLNVVSGGPAKSDLWMQMHTDVSNVPISFTRVSDGPVLG
                     SAMLAAIGAGLYPDIPAAAESMVHTADTIEPDPESHEEYRFYVDRYMEAYPQMKELMH
                     KTERHVAGGGSGAGE"
     gene            9961..11385
                     /locus_tag="VN178_04500"
     CDS             9961..11385
                     /locus_tag="VN178_04500"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HWS80264.1"
                     /translation="MGEQLIEAALREATDTRRVNIGVGALASVAGVFLEIFGDSPAVI
                     VADGTTFEVAGEEVRRRLERAGHTLLEPYVFPAEPSLYAEYSNVETLRDSLRERDAIP
                     VAVGSGTINDIVKRAAHECERPYMCVATASSMDGYTAFGASIEKDGLKQTLSCPAPRA
                     VVADLEVTKHAPEDMTASGYADLLGKVTSGADWLVADALEVEEIDPVGWSLVQDHLRG
                     WTDSPAGLRVGDQGSTGRLLEGLILSGLGMQSYQSSRTASGAEHQFSHLWEMEGLGHG
                     EGHSGTLLSHGFKVGVGTVAIAALYELTLERDLGALDIGSLCDSWPTREAMERSVRAQ
                     HTSQDLRRGAVEQTLAKYISAGELAQRLELLRDVWTGLREKVRVQLMPAEQIREMLRA
                     AGCPTGPVEIGLRWKDFEATYSRARTIRKRYTVLDLAFEAGILEECVDELFAPDGFWG
                     RTAASQTVAEGTRVAGGFRPSGEA"
     gene            11382..12461
                     /locus_tag="VN178_04505"
     CDS             11382..12461
                     /locus_tag="VN178_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HWS80265.1"
                     /translation="MTTVSVLGSGIMATALAFPLTDNGHEVRLIGTHLDREIIDSIKQ
                     RRVHPGLDLEVPQAAKAFQLEEAEEAFDGADVVMSGVNSFGVRWAGEHLANLLKPGMH
                     VLSIAKGMEAAETGDLRILPEVLAGEVPEGLREQVSWSAIAGPSIAGEVAVQRDTCVL
                     FTGRDASVLEEMAGLFRTSYYRVWTSTDLVGVEVCAAAKNCYALGAGFMEGILDREGE
                     SEAPYRNYNYGAALFGQATRELGRFMELLGGERETPYGLAGVGDMFVTSMGGRNVKVG
                     RFVGSGLRFSEARGRMPGVTLEGAAAIEVIGGALPKLTRRGVIEPQDFPLMRHLHAVV
                     ALDEPLNMPWSTFFRGESEYETGKG"
     gene            12464..14182
                     /locus_tag="VN178_04510"
     CDS             12464..14182
                     /locus_tag="VN178_04510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWS80266.1"
                     /translation="MSSRIPQDIDRQAFDLIVIGAGINGAGVARDAAMRGLRVLLLDK
                     GDISSGTTQWATRLIHGGLRYLEHYEFALVRESLRDREAILHIAQHLVKPLGFLVPIY
                     DRSSRGPLMIRLGMNVYDVLSYDRSMDRHKMLSRKEVQEREPGLNPDGLKAAAFYYDA
                     QVEYAERVAVENAISARDNGATIITYANVDRLILENGDVKGVEFIDVFGGGRYEVRAP
                     VTVNVAGPWVDEVLADIEGHGPGENDERFMGGTKGSHLVVDPFPGAPRGEALYVEARK
                     DGRPYFIVPWNGRYLIGTTDLRYEGDLDYVSASEEEIQYLLDETNYVIPGAQLSRDDV
                     LYTYAGVRPLPHQPEGETGGVTRSHVVFDHAKGGSAAGGKITRSDVELPQVGGLLSII
                     GGKLTTYLNLSRQTVDAVVKKLDRKAPKSTSRKVPLPGSETSDFEAFAAGFKSASTIP
                     EVLSSRLLKLYGVRAAEVLEIGEDDPELLTPLSPTVSVETALIGAEVIWAFREEMAQT
                     LSDVLLRRSMAGYGPRVALDVVDAAAEVAVKHLGWEQERAEREAREYREWIVRYTPKE
                     LRDREP"
     gene            complement(14416..14820)
                     /locus_tag="VN178_04515"
     CDS             complement(14416..14820)
                     /locus_tag="VN178_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS80267.1"
                     /translation="MTVVEFGRRGRVERKGMGMYGTVGGDPGFVEGSIDRARGTRSDK
                     PDSEMVARAEALGSWIREHREAFACSQQEFADESGISRATLSKIEQGHIYPSMRVRRK
                     LAKALGVGPQELWNLRSEVLAGRDGESTRQAS"
     gene            15043..15402
                     /locus_tag="VN178_04520"
     CDS             15043..15402
                     /locus_tag="VN178_04520"
                     /inference="COORDINATES: protein motif:HMM:NF038048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HWS80268.1"
                     /translation="MKLAEALANRADLQRRIEQMRGRLQKSALVQEGESPPEDPQELL
                     DETERLVSELEGYVRRINRTNLSATLADGETTLTDALARRDALMLRYGAIKTLVSTAS
                     DRLPRYGRRSASYPPSR"
     gene            complement(15384..15587)
                     /locus_tag="VN178_04525"
     CDS             complement(15384..15587)
                     /locus_tag="VN178_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80269.1"
                     /translation="MFREPTPLSAFATTREDVGYLLENRRPRLTLREIHGPVSLLDGG
                     VELAPLSRQFVHPAPQGSYLDGG"
     gene            complement(15837..16892)
                     /locus_tag="VN178_04530"
     CDS             complement(15837..16892)
                     /locus_tag="VN178_04530"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HWS80270.1"
                     /translation="MRTAVGWIVLGVGSLLAGLLAQSAGLPAGWLVGPMLVALALALV
                     WEEHPTVPRWGRIASLAVVGGMLAATFRPSVLPLVAGHWLPVSLVVGGTLLLSLGAGL
                     LLSGLVPIDRKTAALGALPGAASGMLAMSDPLGADARLVALMQYTRVVVVVVTATLIG
                     RLASGAAPQQISGQGLQAAPEGVDLLVQGTVPTYAVTVLVAVLGAWAGTRLRLPAGAL
                     LGPLILGVALAELGVVRLAWPPGVPQAAYLVLGLWVGLLFDGASVKRAGRLFPSVLAS
                     TVGLVLACAVLGWALAALTGIDGMTAYLATTPGGIESVAIVALGTESDAPLVLAVQML
                     RLLAVVFAGSLLGRWWS"
     gene            17062..>18053
                     /locus_tag="VN178_04535"
     CDS             17062..>18053
                     /locus_tag="VN178_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80271.1"
                     /translation="MDGARTARIPARALTVVFGLIFVATLGAGALTVGTGAQGEPPER
                     LTEKELLSRVSAAPESAPDFRATVTVEQTLVPEGLLGASEGGDTGDSGPRTARVWRGG
                     PDRLRAELQGENGDRVLVRNGDRVSIYDGASNTLEIAKKPDDAASGADGLPGASPEEI
                     DKLLAEIAPSSKLTTGAPVEVAGRWAYPLTLEPRDKDATLVERAEALVDAEAFVPLLF
                     ELYAEGRPEPVTRYETRDFQVGPVPDAPFEFETPPDATVERPEPREDRADEYRAGDEP
                     RKVASVEEAGELAGFAVQALPEVPGGRELEKIVVADDGAVLFYGSDWGSVVLAQ"
BASE COUNT         3505 a         5647 c         5905 g         2996 t
ORIGIN      
        1 agccagactc gtatgagcat cttggccacc cctgtacaag cgaagaaagt ggcccaactc
       61 tttttcatac cgttccaagt agtctcccca agcatgtaga tacgtctcac cggccagggt
      121 aagcccgtac ccttcggccc ctgagccgga agggacgccg tcgggcacca agggatcctc
      181 ttcgaggagg ccctcttcct tcaactgttg catggtcttg tgtaactcct cgggggtgac
      241 ggcgccgaag cccagatcac cgagcctctc ggcgagtccg tctatgcgga tgtcgccgtc
      301 tcgcgcgtac agaaggagaa agagcgtgag ccaccgcgcg ttgggcaagc ccgtgatctc
      361 tgttggatgg agctctgttc cgtcttgcga tctgtgaaaa ccgtccatac ttcttcccct
      421 ttgcgggtga tgccctctgc ggcgcaaggt tacctgttgc ggaccacatc actactaata
      481 gcacgccagc cttcgcacca taagtccgcg atgattccgg gcgagaaaaa tgacccaaag
      541 tatgcagaga atcgtcagta cggagactgg tttagagcct tcggacaggg agacggctcc
      601 ttatcccccc tatacgttac tgcaggcggg gctgacggcg caggcgttcc tggatccagg
      661 ccgccctggc gttttccgac tctatcctgg cctgcctgtc atccctggtg atctcgtcta
      721 tgtgcttgaa ccagacctcg ttaccgctac ggagttccac cagtcctctt tcggccttgc
      781 gctgatcatc ttcggctatg cgttgcagct cctcttgtga ctcgccgggg agcgcccgtc
      841 cgagtgcccg gcccagttgc ccgtttctac gttgctccag ctcccggcgt tcgacctcca
      901 tgtactgttc ccacagctcc tggtgcgtct tgcgtgattc ctcgtccata agcttcctct
      961 taccagtata cgaacggccc tcgacaggag gcgtcgtgcg taaccaggtg cagcccgcgc
     1021 cacgtctcga cgtcttcagc taccgcattg tacatccaac gacgccccgc atgcggtcgc
     1081 gcctgtgtcc gcagcattgt aaaattacaa ggttgctata ctctgcccgc agaggttgtt
     1141 tttcggggat cgtgggcacc gggagaacat cagcacgaca gccggcaagg aggagcattg
     1201 gcggacagct cgtttacgca cctcgtcgtg gtcgggtcct ccgcaggcgg gatagcggcg
     1261 ctctccgggc ttgtttcttc cctacccgat gaattcgatg ctcccatcgt cgtggcccag
     1321 cacctcgacc ccaagcgcga gagccatctg caggagatac tcgcgcgcaa gagcccgcta
     1381 ccagtaaaga ccgtcaccga gcacgaaccg cttcaggctg gcgtggtcta cgtggttcct
     1441 gccaacagac acgtgaacat atccgactcg gagatagagc tcagctccga gcagtcctcg
     1501 aggcagccca tgccctctat aaaccttcta atggagaccg cagcaggggt ctacggggag
     1561 aacctcatcg ccatagtcct gagcggcacg ggcacggacg gcacagaggg cgcacgcacc
     1621 gttagccagg ctggaggcac ggtcatcata caggatcccg agaccgccga attcggcgag
     1681 atgccacgct cgctagcccc gagcaccgtc gacatcgtct cttccctgga ggggatcggg
     1741 ccagtactga agagtttgat ctcgggcgaa gcggcggtcg aggagatctc ggagaacaaa
     1801 gggcgcgacc tggagcggtt cctcgaggag ctgcaccgca accgcggcgt ggacttcagg
     1861 agctacaagc ggcccacgat catgcggcgc ctggctcgca ggatggccgc caccaactgc
     1921 cagagccttg atgagtacta cgggtatctg gaggagcatc cggaggagta caggcagctc
     1981 attggcgcct tcctcataaa ggtgacagag ttcttccgtg acccggaact cttcgattac
     2041 ctcagagagg aggttctgcc cgagcttatc ggggaggtcc gcgaagggga gaaacagctc
     2101 agaatctggt ctgctgggag cgccaccggc gaggaagcct actcgctggc gatcctcctc
     2161 tccgaggtgc ttggccagga ggctggcctc ttcaacgtac gaatctttgc cacggacatc
     2221 gacgagggtg ccgtaagatt tgccaggcgt ggcatctacc ctccctcggc gcttgggggg
     2281 ctctctgaag agcagatcag gcgctacttc gtcgaggagg acggttccta ccaggtaaaa
     2341 aagcagatcc ggggcatgat cgtgttcggg gagcacgatc tggcccagcg ttcgccattc
     2401 cccgagatag acctggtggt aagccgcaac gttttgatct acttctcgaa cgaactgcaa
     2461 cgtagggcgc ttcaactatt cgcctactcc ctgcgcgacg gaggctacct ggtgatgggt
     2521 aaggcggaat ccgcctccct tttgcccgat ttgttcgcgt ccgtagatcg gcacaacaag
     2581 gtctatcgcc ggcacggaca acgcttcctc ctgcctccca ctctcccgac gagcccaata
     2641 ccaagggcgc gcctggggcg gggatctaga gatcagggcg cccccaggcg ccaggccgag
     2701 acgccacagc taccgtctgc cgccttacgg gacccgggcg aggacttgct caaccagctg
     2761 cctgttggcg tcgcagtcgt gacccgcgat tacgctatcg aggccatcaa cgtcgctgcc
     2821 agggagatgc tcgctatccc cagcgtgggt gtagggcagg acttcctgca cgcgatgcaa
     2881 gaggcacctt acgcggaggt ccgcaggacc atagacgagg ctttcaggga aggaaggatc
     2941 acacaggccg atgagttcgc cgtcgaggag gccgccaccg gcgagcccag gtatctacgg
     3001 ctcacctgcc atccccggcg cgccgagacc gaggggcaac gggtgaggcg cgtgctagtc
     3061 gcggtcgaag acgtgaccgc ggaggtacgg atgcggcggc tcgcggagaa gaacctcagg
     3121 ctcgaagagg ccaaccggga gctaggccag ctcaacgagg aacttcaggc cgcccacgag
     3181 gagtctctgg tgaatacaga ggaggcccag gcctcggccg aagaggtaga gacgctaaac
     3241 gaggagctcc aggcgaccaa cgaagagcta gagaccctga acgaggagct acaggccacc
     3301 gtcgaggaac tgaacaccac gaacgacgac ctgcaggcca gggccacgga gctgcaggac
     3361 ctggcgcaga gcagggaaga aggacgccgg agggcagaag agctcgtcga ccagctgcag
     3421 gaccagcgtt cccggctcga agccatcctg gcgaacatct ccgacgcggt gctcgccgtg
     3481 gataccgata ggcagacatt gttcagcaac cacgtcttcg aggaggtctt cggggagcac
     3541 accggcgcgg aacaaggcca gctcggcttc acagaggtgc tggacgagcg tggcgagaag
     3601 ctaccatcgg aagcgacgcc gtcgtcgcgg gccgccaggg gagagtcgtt cgccatgcgg
     3661 ttcaccgtcg ggggcctgga cggcgttccg cggtgcttcg aggccagggg ccgaccaatc
     3721 gaaggcgatg gcgttgcggg aggagtactc gttatccgcg aggcaaccga tggacaggat
     3781 tagagatcct gctatgcgga aaccggactg gaacacctgg gcggccggaa gccggggcgt
     3841 cgaagtagag cgcctggaac aggaactggc ggagtcgcgt gccagcgagc gccgagcccg
     3901 cctcgaggcc gcagagacaa gagaacgttt cgagcgcctt ctcgacgcga gcaggaggtt
     3961 cacccgcacg ctgtctgaca ggaggcggca gcaacagacg agccgccagt tccttgcagc
     4021 ccagcacgcc attgacggcg tactggccga gtccgccggc ctcgaagatg ctgccgcggg
     4081 cgtcttgagg acgctcggcg agaaccttgg ctggcaggtg gccgtattct gggtggctgg
     4141 agcggagacg ttgcgttgca tcgagctctg gcaccggcct gatgccgccc ccgacggttt
     4201 cgcgatggcg tgccgccgga cgaggttcat gcgtggtgag ggcctgcctg gtagcgcctg
     4261 ggcacagaac agacccgtgt ggaccgaaga ttcgccacga gatgagaggt tcgtcgggga
     4321 gaccgtttcg tcggggtacg tgctgcgggg cgccctcgcg tttcccatca ccgggggtgg
     4381 cggacctcac gggatcatcg agctcttggg gagcaaaggc ctacaccccg atcaagaact
     4441 gctctacacc gttgggctca tcggtggtag gctcggtcat ttcgccgacc gccgtaaggc
     4501 ccagaaggac ttgcgcgagg ccgaggagcg gttgaggctg gcgaccgagg ccggacgcgt
     4561 gggcctgctg gattgggacg tactgaccgg cgagagccgg tgctctggtg cgatggccga
     4621 gatctacggc taccctccgg gcgagttcaa cctctcttac gtggagttcc tcgagaaggt
     4681 ccaccctgac gaccgcgtgc gcgtgcgcag cacgctggac gccgccgtcg cagctggcgc
     4741 gccgcacgaa ctggagttcc gcatcgtcag acctaccggc gacatccggt gtgtccacgc
     4801 caagggccgg gttcacaggg acgagaaggg agtgttcgcg cgggtgctcg gggtctccct
     4861 agacgtcaca gaacgaaagc aggcagaaga ggagcgagac cggcttcact ccctggaggt
     4921 ggcagcccgt gccgaggccg ccgagcggaa caggatcagc cgcgagctcc acgatagggt
     4981 ggcccactcg atgggcgtcg cccaccagag cctccaactc tacgaggcgc tcgcggagaa
     5041 ggatcctgtg cgggcccacg gtaagctgcg cacggcgaag gagatgatga agaccgcgct
     5101 ggagcagacc aggaacctct ccatggaact caggcgctcg gagacggagc acggcctcat
     5161 accggccctg caggatctcc tggaggtcgc cgtgcctgaa ggcgtccgcg cggacctctc
     5221 cacctcggga gcggagtcgc ggctctcgga ccaccaacgc gggcaactct atctgattct
     5281 gcgggaggcc gtccgcaacg ccgtcaggca ctcgggctgt cgcactctga ggatcgggct
     5341 ggatataacc tcggagaacg tctttggcta cgtcgaggac gacggccgcg gtttcgaggg
     5401 caacggcggg accagaggcg gcctcgggct ccgctccata agggaacgcg tggcgctcct
     5461 gcaaggaacg gtggaggtat actcttcccc ggaaggcggc gcgagagtgc aggtgcaact
     5521 cccgcttcgg aacggaggag ggtgagttgg cggacagcaa gataagagtg ctgctggccg
     5581 acgatcacgc catgttccgc cagggcataa aggagatgct ctctaccgac gagaagatag
     5641 aggtcgtcgg tgaggcggag aacggacggg aggccggcac tctcgccgag aagctcacgc
     5701 cagacgtagt gcttctggac gtggagatgc cggtcatggg ggcgaaaggg gcgatggagc
     5761 gcatgatgaa gcactcgccg cctcccaggg tggttatcgt caccatgtac gatgacccca
     5821 ggttagtgcg cgagctgatc gggctgggtg ccgtagccta cctggtaaag agcgccacca
     5881 tggaggaact gcacgcggcg gttcacaccg ccgcaaacag ccccgtcggg cccgatcaga
     5941 atgtcgtcat agtcacccct ccgaaggcgt tcgagaaccc ggccgaggcc gacggtctct
     6001 ccgagcgaga gctggaggtg ctgctcatgg tcgcccgcgg gatgagcaac aaccagatcg
     6061 ccatctcgct acacctctcg gaagcgaccg taaagcgaca cctggccaac atatacccca
     6121 ggatcggggt ctcttcccgc ggtgaagcag tgagaaaggc cctctccaat cgttggatca
     6181 cagcccgcga tataaccggc ggcggagagt tgcgagaagt ttagagcggg ttactgtgcg
     6241 cctgctctac cgagcagcga acgccaccac ggtggggggg aagagatctc ttccaggtct
     6301 tctggccaga acaacctcct gtgtccgccc gaaaagcgca catctacggc tatgaatccg
     6361 tcccccccgt aatggtccgc gatcttaccc accatgccgc gtcgctcggc gactctgtgg
     6421 cgctcgcgca ccctcaccgt cgtaccgata ttcacctttt ccggctccat gacgcccccc
     6481 tccgccgcca ggcggaaaaa tcctcccttt cctctcccga gtattcaccc accctcctac
     6541 agggcggcga agtcccaaca tttcaggatc ttcaccgata accgtcgagg tgtcggtatc
     6601 atcagataca attggcgcat gtaggaagcc atgtagttcg aggagagccg gttttcggtt
     6661 ggatgaggac cacacgacgc ttctttccgg ggtaggccta ttcgcatcgc tctcacaaga
     6721 gcagttgggg aggatcaccc acggcatccc ggccaagtcc ttcggggtgg gcgagcacgt
     6781 cttcaccccc acgtacaggg gcggggtatt cttcttgctc ctcgcagggc gggtacgcat
     6841 ctaccgcctc gaagccagac aggaagtgac catatgcgtg ttggaggccg gggagatgtt
     6901 cggcgaggcg gcctttacgt cgagggatgg gaagggctct tatgctcagg ccatagcggt
     6961 ttcgaaggtc gccttcctga atcgctcgac tttctacagg ctcatccaga gagaccccga
     7021 attgggcata agggccatag agctcctggg cgagcgcctc tccttctacg agcagagaat
     7081 cgccgacctg ggcctcaaga aggtgcctgc ccggctggcg agcctcatcc ttcagctggt
     7141 ggagaaagag gggatcgtga ccggcaaagg acgctaccgc ctcagcaccc actacaccca
     7201 cgaacagctc gccctgatgg tcggggccaa acgcgtcgcc gttagcaggg cgatgaaggg
     7261 gctcagggag gccggggccg tagagaccgg gcgccggcgc atcgtggtca aggacgccaa
     7321 ggcgctcggg aggatcgccg acgaagtggc ttgagggaga tccctccaag gacatgcgta
     7381 ggagctgcca ctgtttcgga cggccatgcc cggtaagatg gtcgcgaggg atttcccggg
     7441 cgctgtagtg gaggaggttg cgtggttcag cgtagcgagg ccaggtcctc gggcgcgcct
     7501 gagaggccgg acaggctgta cgagggctac gtcttcgatc tcgacgggac catctacctg
     7561 ggagacgggc tgctccccgg cgcgaagcgt ttgatcctca agctgcgcga gctgggcaaa
     7621 cgcgtggtct tcgtctccaa caaccctacg aaagacccga agatgtatgc tgagaagctc
     7681 ggcgcgctcg gcctgaagac accggcttcc gagatagtga acaccgtcgt caccatgaca
     7741 gagtggttgc tcaggaagcg ccccgaagca acggtcttcc ccataagcga ggagccgctc
     7801 aagaactccc tctccgaggc cgggatcagg atgtccgagg atcccgagga gatcgacatc
     7861 gttatcgcga gctacgaccg ctccttcgac taccgcaagc tccagatcgc cttcgacgcc
     7921 atctggttct acgggcgggc gatgctcgtc accacgaacc ccgaccgcta ctgccctctc
     7981 cccaacggca ggggcgagcc cgacgcggcc gcgatcgtcg cggccataga ggcgtgtacg
     8041 gggtcgcagt gccgggtcaa cgtcggcaag cccgatccca taatgttgga gacggtgatg
     8101 gacctgatag ggctcgatgc gaaagaatgt gtgatgagcg gcgaccggct ctacacggag
     8161 atacggatgg cgaaaggcgc ggggatacct tctgccatcg tcctgaccgg cgagactacg
     8221 gaagaggacc tggccgggga gccggtggag aacctgcctg actacgtcct gaagaggata
     8281 gatcaactcg tcccgcgcga cctgtgggac gagttcggct gggaggatgg gtgagaggtt
     8341 cgccgcgata cactgggctg gcccctttcg agagaggagt gcaatgatgg ccgatggtcc
     8401 ttaccttttg ggcatcgact tcggaacggg gggagcgagg gtcggcatct tcgaccccga
     8461 agggacgccg ctggtgttca gcgaacggga gtacgcgctg aagcacccgc gtcccggctg
     8521 ggccgagcag gacccagacg agtggtggtc gtgcctggtt gcggcggtga aaggcgcgat
     8581 ggaagagagc agcatctccc ctgaggacat agccggcatc tcgatcgacg ccatgagcag
     8641 cacggtcctc gcgatcgacg aaaggggcag gcatctgcgc ccggctatca tgtggatgga
     8701 cgtgcgctct tcggaggagg ccgacaggat tgcaggcacg aacaacaacg ccctcaagta
     8761 caacgggtac ggggccgtct cggccgagtg ggggcttccg aaggcgctct ggatcaagga
     8821 gaacgagcct gaggtctaca gggacgccag gcacatctgc gactgcggcg actggctgat
     8881 ccaccgcctc accggcgaat ggacgtcctc tatcaacata gtcagttcga agtactacta
     8941 cgaccgcgac gaaggcggct acccagagag cctctacgat gcggtggacg ccacagactt
     9001 gctcgagaaa ttccccggcg atgttctcga tctcggcacc ctcgccggag agttacgcag
     9061 tgaggcggcc gaggaactcg gtctgaagac gggcacgccc gtcgccgagg ggggagtaga
     9121 cgcctacgtc ggggcgatcg gcctcggggt ggtcgagccg gggaagatgg ccctgatcac
     9181 aggctcttcg cacgtcatga tcgggcagac cgcgacccct atccacgatc ctggtttctg
     9241 gggcgcctac acggatgcca tgatccctgg ccagtacacc gtcgaggccg ggcaggcctc
     9301 cacgggttcg atagtcgcct ggttcaagaa ccagttcgcg ggcgacgcgg ccgccgaagc
     9361 cagagagcgt ggtatagaca cctacgacgt cctgaccgag ctggcgcgcg acgtgcccat
     9421 aggttcggac gggctcatag tgctcgacta ctttcagggc aaccggtctc cttataccga
     9481 ccccctggcg cgcggcatga tgtggggact gaccctgagc cacaccccgg gacacgtctt
     9541 tcgcgccatc atcgaaggca tctgctacgg cacggagaac atactccgca ccatgcgcgg
     9601 gcaagacttc gagccgaagc tcaacgtggt ctccggtggt cccgccaaga gcgacctgtg
     9661 gatgcagatg cacaccgacg tgtcgaacgt gccgatctcg tttaccagag tgagcgacgg
     9721 tcccgtcctc ggctcggcga tgctggccgc catcggggcc ggtctctacc ccgacatccc
     9781 tgccgcggcc gagagcatgg tccacaccgc cgacaccatc gagccagacc ccgaaagcca
     9841 cgaagagtac agattctacg tggaccgcta catggaagct tatccgcaga tgaaggagct
     9901 gatgcacaag acggagcgtc acgtagccgg cgggggatcc ggggcggggg agtagactct
     9961 atgggcgaac agctcatcga agcggccctg cgggaggcta cggacacacg gagagtgaac
    10021 atcggggtcg gcgcgctcgc ctccgtcgcc ggcgtctttc tggaaatctt cggtgatagc
    10081 cccgcggtca tcgtcgccga cgggacgact ttcgaggtcg cgggcgaaga ggtccggcga
    10141 cggctcgaaa gagccgggca caccttgctt gagccctacg tattccctgc ggagccgtct
    10201 ctttatgcgg agtactcgaa cgtcgagacg ttgagagact ccttgcggga gcgcgatgcg
    10261 atccccgtgg ccgtggggtc ggggacgatc aacgacatcg tcaagcgggc cgcccacgag
    10321 tgcgagaggc cttacatgtg cgtcgcaacg gcctcctcga tggacggcta cacggctttc
    10381 ggcgcttcga tcgagaagga tgggttgaag cagacgctgt cctgccccgc tcccagggcc
    10441 gtggtcgctg acctggaagt aacgaagcat gccccagagg acatgacggc ctccgggtac
    10501 gcggatctcc tcggcaaggt gacatcaggc gccgactggt tggtcgccga cgccctggag
    10561 gtcgaggaga tcgatcccgt aggctggtcg ctggtgcagg atcacctgcg cgggtggacg
    10621 gacagcccgg ccgggttgcg cgtcggagat caaggatcca ccgggcgcct tttggagggt
    10681 ctgatcctgt ccggcctcgg gatgcagtcc taccagtcct ccaggacggc ctcgggcgca
    10741 gagcaccagt tcagccacct gtgggagatg gaaggcctgg gacacggcga gggtcacagt
    10801 ggaactctcc tctcccacgg cttcaaggtc ggggtaggca ccgtcgccat agccgcgctc
    10861 tacgaactga cattggaacg tgacctcggc gccctcgaca tcgggtcgct ttgcgactcc
    10921 tggcccacca gggaagcgat ggaacgatcc gtacgggcgc agcacacgag ccaggacctg
    10981 aggaggggcg ccgtcgagca gaccctcgcc aaatacatca gcgccggcga actcgcgcag
    11041 aggctggaac tgcttcggga tgtatggacc ggactgcgcg agaaggttag agtgcagctg
    11101 atgccggccg aacagataag agagatgctt cgggccgccg gttgtcctac cggacccgtc
    11161 gagatcgggc tccgctggaa ggacttcgag gcgacgtact ccagggcgcg aacgatccgc
    11221 aagcgttata cggtcctgga cctggctttc gaggccggca tcctcgaaga gtgcgtggac
    11281 gaactgttcg caccggatgg cttctggggc cgtacggcag catcgcagac agtggctgag
    11341 ggaacgcggg tggccggcgg gttccgacct tcgggagaag catgacgacc gttagcgtac
    11401 ttggctccgg cataatggcg actgccctcg ccttcccgct cacggacaac ggccacgagg
    11461 tgcgcctgat cggcacgcat ctcgaccgtg agatcatcga cagcatcaag cagaggcgcg
    11521 tgcatcctgg cctcgacctc gaggtccccc aggcggccaa ggccttccag ctcgaggagg
    11581 ccgaagaggc tttcgatggc gccgatgtcg tcatgagcgg ggtcaactcg ttcggggtgc
    11641 gctgggccgg cgaacatctt gctaatctgc taaagcctgg catgcacgtt ctctccatcg
    11701 ccaaggggat ggaggccgcg gagaccggag atttacgcat cctccccgag gtgctcgccg
    11761 gtgaggtgcc cgagggtctt cgggagcagg tgtcctggtc ggcaatcgcc gggccctcca
    11821 tcgccggcga ggtcgccgtg cagagggaca cgtgcgtact cttcaccggc cgcgacgcca
    11881 gcgtgttgga ggagatggcg ggcctcttcc gtacttccta ctatcgtgtg tggacctcga
    11941 ccgacctcgt tggcgtcgag gtgtgcgcgg cggccaagaa ctgctacgcc ctcggggccg
    12001 gattcatgga gggcatactc gaccgtgagg gcgagtcgga ggcgccctac cgcaactata
    12061 attacggggc cgcgctcttc ggccaggcga cgcgggaatt gggacggttc atggaattgc
    12121 tgggcggcga gcgtgagacg ccatacgggt tggccggggt cggcgacatg ttcgtcacga
    12181 gcatgggagg acgtaacgtg aaagtggggc ggttcgtcgg ctcgggactt cgattctccg
    12241 aggcgcgcgg gcggatgccg ggggtgacgc tcgaaggggc ggctgccatc gaggtgatcg
    12301 ggggcgcgct tccgaagctt accaggcgtg gtgtcatcga accccaagac ttcccgctga
    12361 tgcgccacct gcacgccgtg gtcgcattgg acgagcctct caacatgccc tggagtacgt
    12421 tcttccgggg cgaatcagag tacgagacgg ggaaaggata gagatgtcat cacgcatacc
    12481 gcaggacata gacaggcagg ccttcgatct gatcgtgatc ggggccggca taaacggcgc
    12541 cggcgtggcc cgtgacgccg cgatgcgagg cttgcgggtt ttgctcctgg acaaaggcga
    12601 catctccagc gggacgacgc agtgggcgac gcgtctgatc cacggcggtt tgcgctacct
    12661 ggagcactac gagttcgcgc tcgtaagaga gtctttgagg gatagggaag ccatactgca
    12721 catcgcgcag catctcgtca aaccgctcgg cttcctggtt cccatctacg ataggagctc
    12781 ccgcggaccg ctcatgataa ggctcggcat gaacgtctac gacgtgctct cctacgacag
    12841 gagcatggac cggcacaaga tgctcagtcg caaggaggtc caggagcgcg aaccaggcct
    12901 caacccggac ggcctcaagg cggccgcttt ctactacgac gcgcaggttg agtacgcaga
    12961 aagggtcgcc gtagagaacg ccatctcagc ccgcgacaac ggcgcgacca tcatcaccta
    13021 cgcgaacgta gaccgcctga tcctggagaa cggcgacgta aagggcgttg agttcatcga
    13081 tgtcttcggg ggcgggagat acgaggttcg cgcccccgtt acggtgaacg tggcgggccc
    13141 ctgggtggat gaggtcctcg ccgatatcga aggacacggc cctggggaga acgacgagcg
    13201 ctttatgggc ggcaccaagg gcagccatct cgtcgtggat cccttccccg gcgcccccag
    13261 gggcgaggcc ctctacgtcg aggcgcgcaa ggacggcagg ccgtacttta tcgttccgtg
    13321 gaacgggcgg tacctgatcg gcacgaccga cctccgctac gagggtgacc tcgactacgt
    13381 ctcggcgagc gaggaggaga tccaatacct gctcgacgag accaactacg taatcccagg
    13441 ggcgcaactc tcccgcgatg acgtgctcta tacctacgcc ggagtgaggc cgctgcccca
    13501 tcagcccgaa ggtgagacgg ggggcgtcac ccgtagccac gtcgtcttcg accacgcgaa
    13561 gggaggttcc gcggcgggcg gtaagatcac gcggtcagac gtggagttgc cgcaggtggg
    13621 gggactcctc tccatcatcg gcggcaagtt gaccacctat ctgaacctga gccgccagac
    13681 ggtcgacgcc gtggtcaaga agctggatcg gaaggcgccc aagagcacct ctcgcaaggt
    13741 ccctctgccg gggagcgaga cctcggattt cgaggctttc gccgcgggct tcaaatccgc
    13801 tagcaccata cccgaggtcc tctcgagcag gcttctcaag ctctacggcg tcagggccgc
    13861 cgaggtcctc gagataggcg aagacgatcc cgaactcctt acgcccctct ctcctacagt
    13921 aagcgtcgag acggcgttga tcggggccga ggtgatctgg gctttccgcg aggagatggc
    13981 ccagacgctc tccgacgtac tcttgagacg ctcgatggcc ggttacggcc caagggtcgc
    14041 tctcgacgtc gtcgatgccg ccgccgaggt cgccgtcaaa cacctcggat gggaacagga
    14101 gcgcgccgaa cgcgaagcgc gagagtaccg cgagtggatc gtacgctaca cccccaaaga
    14161 gctcagagat cgcgaaccct gaccgactca ggccgggctc ggcgcgtatg ctccgataag
    14221 agatccaccc gggccaacgt cctgatacgg aatccggccg attggttcgt gtcgtttgta
    14281 ttgcgccgcc cacccgtcag ggctcgcacg aactctctcc ggcccctgca caatagggcg
    14341 agggtgccgt cctcgatcat ctggggaagc gggtcggaaa ggaagaagcc tcccgcctac
    14401 tctacgagag gttctttacg atgcctgcct tgtgctctcc ccgtcccggc ccgcaaggac
    14461 ctcagaccgc aagttccaga gctcctgcgg accgacgcct aacgccttcg ccagctttct
    14521 gcgaacccgc atagacggat agatgtggcc ctgctctatc ttggataacg tcgcccggct
    14581 tatgccgctc tcatcggcaa actcttgctg gctacacgcg aacgcttccc tgtgctccct
    14641 gatccaactc cccagcgcct ccgcccgcgc caccatttcc gagtcgggct tatccgatcg
    14701 ggtgcctcgc gcccggtcaa tgctcccttc cacaaaacca gggtcgcccc caacggtccc
    14761 gtacatgccc atacccttcc tttcgacgcg tccgcgtctc ccgaactcga caaccgtcat
    14821 cctgcaaatc cctctctgct cgtctctcac cagcgccttc atgcgcccac gcgacaatta
    14881 tacgcctaaa accggtcgac tgcgagaaca ataatggcta aaatattgtc taatttatgt
    14941 tgacataacg tccaatacat gtagaatggc tcctgttcgg cggagatgcc cttgcgggaa
    15001 gatcacgcgg tggacgataa ggacgtaagg aggtgaaagg taatgaagct ggcagaggca
    15061 ctggcgaaca gggctgatct gcagaggcgg atcgagcaga tgcgggggag gcttcagaag
    15121 agcgccctcg tccaggaggg tgagagcccg cccgaggatc cccaggagct cctcgatgag
    15181 acggagaggc tcgtctccga gctcgagggg tacgtacggc gcatcaacag gacgaacctc
    15241 tccgcgacgc tcgccgatgg tgagacgacg ctcacggacg ccctggcgcg ccgcgacgcc
    15301 ctgatgctgc gctacggggc cataaagacc ctcgtaagca cggcgtctga ccggctgccg
    15361 cgctacgggc ggagatccgc atcctacccg ccgtcgaggt aggacccctg aggcgcagga
    15421 tggacgaact ggcgcgagag aggcgcgagc tcgacacctc catccagcag gctaactggg
    15481 ccgtggatct cacggagggt taggcggggt cttctgttct ccagcaggta acccacgtct
    15541 tcgcgggtgg tggcgaacgc cgagagcggc gtcggctctc taaacaaaga cgcatcacgg
    15601 attggcttca gaagccaggt tccagggcgg tgccggggcg gtaccgcggg ggttcgagtc
    15661 cccgttttca ccgacgagcc cagcactcct aacacgggca ccgggcaacg cgcagcgtaa
    15721 ccaccgggcg taggaccatc cgaacgagga cagggcgagg gcaggcaagg ggctaatgga
    15781 gaacagccgt agggccctgg ggcgcgcccc agggccctct cgtctgcacc ggcatctcac
    15841 gaccaccatc tcccgaggag cgagccggcg aagaccacgg ccaggagccg cagcatctgg
    15901 accgcaagca ccagcggtgc gtcggattcg gtaccaaggg ccacgatcgc gaccgactct
    15961 atcccacctg gcgtggtcgc taggtaggcc gtcatcccgt cgatgccggt gagcgcggcg
    16021 agcgcccatc ccaggacggc gcacgcgagt acgagcccca cggtggaggc gagcacggac
    16081 ggaaacaacc gtccggctct ctttacggag gccccgtcga agagcagtcc tacccacagg
    16141 ccgagaacca ggtaggccgc ctgcggcacg ccaggtggcc aggcgaggcg caccacaccg
    16201 agctccgcca atgcgactcc gaggatcagc ggtccgagga gcgcccctgc cggtagcctg
    16261 aggcgcgtcc cagcccatgc gccgagtacc gcgaccagca cggttacggc gtaggtcggc
    16321 acggttccct ggaccaggag atccacccct tccggggcgg cttgcaagcc ctgaccggag
    16381 atctgctgtg gcgcagcccc ggatgcgagc cggccgatca gcgtggccgt cacgacgacc
    16441 acgaccacgc gcgtatactg catcaaggcg accaggcgcg catcggcgcc caaagggtcg
    16501 ctcatggcga gcatccccga ggcggcgccg ggcagagccc ccagagccgc agtcttcctg
    16561 tctattggga caagcccgga gagcaacagt cctgccccca ggctcagaag caaggtaccg
    16621 ccgaccacga ggctcacggg cagccagtgg ccggcgacca ggggaaggac ggacggcctg
    16681 aaggtggcgg cgagcatgcc gcctactacc gcgagcgacg ctatccgccc ccagcgcggc
    16741 accgtcgggt gctcttccca cacgagcgcc agggcgagcg cgacgagcat cggcccgaca
    16801 agccacccgg ctggcagtcc cgcactctgg gccaagagtc cggccagcaa agaacccacg
    16861 ccaagaacaa tccaccctac ggcggtacgc atcgcccttg atcccttgct agtcaccctc
    16921 ttgcttcgtc cttccttcta cggcactgaa actagcgagg ctcggccgga gtgtgcacaa
    16981 acccattcct gtcttccatt cgtaggatag actagaaaga tcaggggggc ttgagtaagg
    17041 tcgacgcgag gaggcgttgt catggatgga gcgaggaccg caaggatccc ggcgcgagcc
    17101 ttgaccgtgg tcttcggtct catcttcgtc gctacgctcg gcgcaggcgc cctcaccgtc
    17161 gggaccggcg cccagggcga gcctcccgag aggcttacgg agaaagaact cctctcccgc
    17221 gtaagcgccg ctcccgagag tgcgcccgac ttccgcgcga cggtgaccgt cgagcagacg
    17281 ctcgtccccg aaggtctcct cggcgcctcc gaagggggcg acaccggaga ctccggcccg
    17341 cgcaccgccc gcgtctggcg cggcggcccc gacagactac gcgccgaact gcagggcgag
    17401 aacggggacc gggtcctcgt ccggaacggc gaccgggtaa gcatctacga cggcgcctcg
    17461 aacacgctcg agatcgccaa gaagccggat gacgcggctt ctggcgcgga cgggttgccg
    17521 ggtgcgtcgc cggaggagat cgacaagctt cttgcggaga tagcgccgtc gagcaaactc
    17581 acgacagggg cgccggtcga agtcgcggga cgttgggcgt acccgctgac cctggagccc
    17641 agggacaagg atgccacact cgtcgagcgc gccgaggcgc tcgtagacgc tgaggccttc
    17701 gtgccgctcc tcttcgagct ctacgccgag gggaggcccg agccggtgac ccgctacgag
    17761 accagggact tccaggtcgg ccccgtccct gacgcgccct tcgagttcga gacgccgcct
    17821 gacgccacgg tcgagcggcc ggagcccagg gaagatcggg cggacgaata tcgtgctggc
    17881 gacgagcctc gaaaggtcgc atcggtagag gaggcgggcg agctcgcggg gttcgccgtc
    17941 caagcgctac ccgaagtacc cgggggccgg gagctggaga agatagtggt cgccgacgat
    18001 ggagcggtcc tgttttacgg gtcggactgg gggagcgtgg tcttggccca gaa
//
LOCUS       DATMVF010000099         4941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_607174, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000099 DATMVF010000000
VERSION     DATMVF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607174"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VN178_04540"
     CDS             <1..793
                     /locus_tag="VN178_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HWS80272.1"
                     /translation="ANGDRFWELSGGVLYCAECGCRMTVHATLDSRNRRRYCYYRCPK
                     RGRHGVQKVCINGKHHRAAEAEAAVWDLVSGLLKDPERLRAGLDEMIEQERAGTRGNP
                     DQEAASWLEKLAEVEQERRSYLRFAAKGHMTDDELDEALADLEETRTTAEEELAAIRG
                     RKEIIEELERDRDALLESYAQLTPEALDALTPEERRQVYGMLRLRVEVAADGTMEAQG
                     ILRDNVRVQDENGRSASEGMFCEHGLVSRSTICWHCTVLGRPRRA"
     gene            760..1221
                     /gene="radC"
                     /locus_tag="VN178_04545"
     CDS             760..1221
                     /gene="radC"
                     /locus_tag="VN178_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWS80273.1"
                     /translation="MHGIGEAKACITLAAVEFGKRLGRVRNPGRPVISSPEDVDGLLR
                     GRIANLDRENFVAVLLNTKNEVIEFPTISVGTLSASLVHPREVFKPAIRASAAGIVLA
                     HNHPSGKVGPSREDREVTRRLKEAAEIIGIEVVDHVILGDGYFSMKEHGIL"
     gene            1271..2299
                     /locus_tag="VN178_04550"
     CDS             1271..2299
                     /locus_tag="VN178_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWS80274.1"
                     /translation="MFGGLFGRDIAIDLGTANTLVFVKGQGIVLSEPSVVAIDTKTDK
                     VVAVGSAAKRMLGRTPGNIVAMRPLKDGVIADFEVTEKMLAYFIRKVQPQRWFARSLI
                     GPRVVVCVPSGVTGVELRAVKEATESAGARQAYTIEEPLAAAIGTGLPVNEAQGSMIV
                     DVGGGTSEVAVISLGSIVTKSSIRIAGDDIDDAISSYIQKEYKLAIGTQTAEQLKMEL
                     GSAFRLDEEESAEIRGRDLVTGLPKTIVITSEEIREAISVPVDAVLAAVRDTLDRTPP
                     ELASDIMDRGMVLAGGGALLRHLDERLRRETGIPVHVADDALMCVAIGSGRSLEEINS
                     YRSALSSA"
     gene            2308..3237
                     /gene="mreC"
                     /locus_tag="VN178_04555"
     CDS             2308..3237
                     /gene="mreC"
                     /locus_tag="VN178_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWS80275.1"
                     /translation="MGRKKSSAASGLAALFILSVVSVALFTIYVKEGDCAQEQGCGPL
                     HTVQLGAAEVLQPARSIFATVFEPLQRANESVRGAFDNSAEQRLERKLQESRALAAQA
                     SRLERENARLRELLEGERSAYEYGPLAQVVAPVGDQFTQRIVLNVGTEDGVEPNRPVV
                     IGENTLVGRTTEVSNHTAQVMLITDQMFAAGVRIIPPAEFDPASGELSPAVTAEDVSY
                     GQGLLETNLEGYFGVDLVSLKARAEEGDFVVTSGRAGDKELLFPPGLYVGKVEAVSSQ
                     DIEQFKKIVVDPAMNPQDLEEVRVIVDWAGREG"
     gene            3247..3762
                     /gene="mreD"
                     /locus_tag="VN178_04560"
     CDS             3247..3762
                     /gene="mreD"
                     /locus_tag="VN178_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWS80276.1"
                     /translation="MEQASVIRAAIVVAIAALLDAVVIPYLTFGFFSPRLTLIAVVFA
                     VAPLRDLQAVLLGFFGGILLDALGTDLFGVGALAGLLAAMLATRASAVRRRGSEKVLF
                     AQVAGLAVAAYDLLSYGARWLAGLGVPQLGEYVVWGVLPDAIINGLLAFLIGGWLLNV
                     VDIKTPHGWEK"
     gene            complement(3991..>4941)
                     /locus_tag="VN178_04565"
     CDS             complement(3991..>4941)
                     /locus_tag="VN178_04565"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWS80277.1"
                     /translation="VGAVVLALLDYRWLGRHLRFVYGAMIVMLVAVLVAGFSVNGAQS
                     WIGVGPVEVQPSEFAKPLSIVVLAGYVAESSFARNMTFLTALGIMSVPVLLVLAQPDL
                     GTALVFGAIFVAVVFVGGARWYQLGGLFAAGCVGVYLTIKFRILEDYQIARLTSFLDP
                     QSVPSVSYQVENSKMAIGSGGLMGKGLRSSGTLGDLGYLPEDRTDFIFANLAEKVGFV
                     GSVVLLIVFFLLVWRVLHAATISSDRFGVLVAVGVATMLTFHALINIGMAMGMMPVTG
                     LPLPFVSYGRSNLLVSMMSVGLVQSIVIQAKLKSEGNPRA"
BASE COUNT          963 a         1512 c         1641 g          825 t
ORIGIN      
        1 cgcgaacggg gatcgcttct gggaactctc cggcggcgtc ctctactgcg ccgagtgcgg
       61 ttgccgaatg accgtacatg cgaccttaga ctcgaggaac cgtaggaggt actgctacta
      121 ccgctgtccg aagcggggac gccacggggt gcagaaggtg tgcatcaacg gcaaacacca
      181 ccgggcggcg gaagcggagg ccgccgtgtg ggatctggtc tccggcttgc tgaaagatcc
      241 tgagcgtttg cgggcggggc tcgacgagat gatcgagcag gagagggccg gtacgcgcgg
      301 gaacccggac caggaagccg cctcgtggct ggagaagtta gccgaagtcg agcaagagcg
      361 gcgtagctac ctgcgctttg ccgcaaaggg tcacatgact gatgatgagc tggacgaggc
      421 actggcggac ctagaggaaa cccgcacgac ggcggaggag gagctggcgg ccatacgtgg
      481 ccggaaggag atcatagagg agcttgagcg agacagggac gccctgctcg aatcctacgc
      541 acagttgacg ccggaggcgc tcgacgccct tacgccggag gagcgtcggc aagtctacgg
      601 gatgctgcgc ttgagggtcg aggttgctgc cgacggtacg atggaagcgc agggcatcct
      661 tcgggataac gtccgcgtgc aggacgagaa cggacgttcg gcgagcgagg gaatgttttg
      721 tgaacacggt cttgtatctc ggtccacgat ctgctggcat tgcacggtat tggggaggcc
      781 aaggcgtgca taacgctggc cgccgtcgag ttcggcaaga ggttgggaag ggttcgcaac
      841 ccgggacgcc ccgtgatatc atcgccggag gacgttgacg gtctcttgag gggtaggatc
      901 gctaatctcg accgggagaa tttcgtcgcg gtgctcttga acaccaagaa cgaagtaata
      961 gagtttccga ctatctcggt tggcacgctt tcggcgtctc tggttcaccc cagggaggta
     1021 ttcaagccgg cgatccgggc aagtgctgca ggaatagtgc tcgcccacaa ccaccccagc
     1081 ggcaaggtag ggccgagtcg cgaagaccgc gaggtcacca ggcggctcaa agaggcagcc
     1141 gagataatcg gcatcgaggt cgtagatcac gtgatcttgg gcgatgggta ctttagtatg
     1201 aaggagcacg gaatactcta gccacctggg tggtggcgcg ggggcgttcg atacggagga
     1261 tgggtcgcgt atgttcggag gactgttcgg gcgagacatt gcgatagacc ttggtacggc
     1321 gaatacgcta gtgttcgtca agggacaggg gatcgtactc tctgagccct cggtggtcgc
     1381 aatagatacc aagacggaca aggtcgtggc cgtcgggtcg gcggcgaagc ggatgctcgg
     1441 tcgcacgccg ggcaatatcg tcgcgatgag gcccttgaag gacggtgtga tcgccgactt
     1501 cgaggtcacg gagaagatgc tcgcttactt tatccgcaag gtgcagccgc agcgctggtt
     1561 cgccaggtcg ttgatcggac cgagggtcgt ggtgtgcgtg ccctcgggcg tgacgggcgt
     1621 cgagctcagg gccgtcaagg aggcgaccga gtcggcgggg gcccgccagg cttacaccat
     1681 cgaggagccg ctggccgccg ccatagggac gggcctaccc gtcaacgagg cccaagggtc
     1741 gatgatcgtc gacgtcggcg gcgggacgag cgaggtcgcc gtcatctccc tcggcagtat
     1801 cgtcacgaaa tcctccatcc gcatcgccgg cgacgacatc gacgatgcca tctcctctta
     1861 cattcagaag gagtacaagc tcgccatcgg cacccagacg gccgaacagc tcaagatgga
     1921 gctcggcagc gccttcaggc tggatgagga ggagtcggcc gagataaggg gccgggacct
     1981 cgtcacggga ctgcccaaga ccatcgtcat aacgagcgag gagatcaggg aggccataag
     2041 cgtccccgtg gacgcggtcc tcgcggccgt gcgggatacg ctggaccgca ctccacccga
     2101 gctcgcatcg gacatcatgg accgcgggat ggtcctcgcc ggtggcgggg ctctcttgag
     2161 gcacctcgac gagcgcctca ggcgcgagac gggtatcccc gtgcacgtcg ccgacgacgc
     2221 gctgatgtgc gtggccatcg ggagcggccg ctccctcgaa gagatcaact cctaccgtag
     2281 cgccctctcc tcggcctaga gcacgacgtg ggtcgcaaaa agtccagcgc cgcaagtggt
     2341 ctcgcggccc tctttatcct cagcgtggtc agcgtcgcgc tcttcaccat ctacgtaaaa
     2401 gagggcgact gtgcgcagga gcaaggttgt ggacccttgc acacggtcca gctcggcgcg
     2461 gccgaggtct tgcaacctgc gcgcagcatc ttcgcgaccg tcttcgagcc cttgcagcgg
     2521 gcgaacgaga gcgtgcgggg cgccttcgat aacagcgcag aacagaggct cgagcgcaag
     2581 ttgcaggagt cccgggccct cgcggcccag gcgtcccgcc tcgagcgcga gaacgcccgc
     2641 cttcgcgaac tcttagaggg agagaggtcc gcctacgagt acggtccgct cgcgcaggtc
     2701 gtcgccccgg taggggatca attcacccag aggatagtcc tcaacgtggg taccgaggat
     2761 ggcgtcgaac ccaacaggcc cgtggtaatc ggcgagaaca cgctcgtcgg gcgaaccaca
     2821 gaggtctcca atcatacggc ccaggtcatg cttatcacgg accagatgtt cgctgcgggg
     2881 gtcaggatca tccctcccgc cgagttcgat ccggcttccg gggagcttag ccccgccgtc
     2941 acggccgagg acgtctctta cggccagggg ctactggaga ccaacctcga ggggtacttc
     3001 ggggtcgacc tggtcagcct caaggcgcgg gccgaggagg gtgacttcgt cgtcaccagc
     3061 gggagggccg gggacaagga gctgttgttc cctcccggcc tgtacgtggg taaggtcgag
     3121 gcggtcagct cgcaggacat cgagcagttc aagaagatag tggtggaccc ggccatgaac
     3181 ccccaggacc tcgaagaggt gcgggtaatc gtcgactggg ccggccggga agggtagaga
     3241 cagccggtgg agcaggcctc cgtcatccgc gccgcgatcg tcgtcgcgat cgccgcgctg
     3301 ctcgacgccg tcgtaatccc ctacctgacc ttcggcttct tctcgccgag gttgaccctg
     3361 atcgcggtcg tcttcgccgt cgccccactg agggatctcc aggccgtgtt gctcggcttc
     3421 ttcgggggca tactcctcga cgccctgggc acggacctct tcggggtcgg agcgctcgcg
     3481 ggcctgctcg cggccatgct cgccacccgc gcgagcgccg tgaggcgaag aggttcggag
     3541 aaggtcctgt tcgcccaggt cgcaggacta gccgtcgcag cctacgacct cctgagttac
     3601 ggcgcccgct ggctcgccgg gctgggagtg ccacagctcg gcgaatatgt tgtctggggc
     3661 gtgctccccg atgccatcat caacggcctg ctcgccttcc tcatcggcgg gtggctactc
     3721 aacgtcgtcg acatcaaaac cccccacgga tgggaaaagt agcatgtcag cacttcagca
     3781 cgccgcccgg cttcgcctgg cggcttgtca gcaattcagc tagtcgtccc gtaacctcgc
     3841 cctgagcgta gagcccgatt gccgcttgaa gcgcctcgtc gtacgcggca gagcgaggcc
     3901 ggaacgggag aagctgacct gctgacaagc gagggcctga aaggcccgaa gcgtgctgac
     3961 aaggcgcgaa gcgccgtgct gactagcttc tcaagcccga gggtttccct cggacttgag
     4021 cttcgcctgg atcacgatgc tttgcaccag cccgaccgac atcatgctta cgagcagatt
     4081 gcttcgtccg tagctcacga aggggagtgg aagcccggta acgggcatca tgcccatcgc
     4141 catcccgatg tttatgagtg cgtggaacgt aagcatcgtc gccaccccga ctgcgacgag
     4201 cacgccgaag cggtcgctcg agatcgtcgc ggcgtgcagc acgcgccaga ccagcaggaa
     4261 gaaaacgatc agtaggacca cagaccctac gaagccgacc ttctctgcga ggttggcgaa
     4321 tataaagtcc gtgcggtcct ccggcagata gccaaggtca ccgagggtcc ccgacgatcg
     4381 aagacccttg cccatgagcc cccctgaacc tatggccatc ttggagttct cgacctggta
     4441 gctcacgcta gggacgctct gggggtcgag gaacgaggtg agacgtgcta tctggtagtc
     4501 ttccaggatt ctgaacttta tggtgaggta gacgcccacg cacccggccg cgaacagccc
     4561 cccgagctgg taccacctgg ctccgcccac gaagaccacg gcaacgaata tggccccgaa
     4621 caccaacgcg gtaccaaggt ccggttgcgc gagcacgagc aagaccggca cggacatgat
     4681 gccgagggct gtcaggaagg tcatgttgcg agcaaagctg ctctcggcca cgtaaccggc
     4741 gagcaccacg atcgacaacg gcttcgcgaa ctccgaaggc tgcacctcga cggggccgac
     4801 cccgatccag ctctgcgccc cgttgaccga gaatcccgcg acgagcacgg ctaccaacat
     4861 cacgatcatc gccccgtaga cgaacctgag gtgtcgccca agccacctgt aatccagcag
     4921 cgcaagcacg acggcgccca c
//
LOCUS       DATMVF010000100         5713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_290077, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000100 DATMVF010000000
VERSION     DATMVF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290077"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..352
                     /locus_tag="VN178_04570"
                     /pseudo
     CDS             194..352
                     /locus_tag="VN178_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            536..844
                     /locus_tag="VN178_04575"
     CDS             536..844
                     /locus_tag="VN178_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80278.1"
                     /translation="MSEPIPGTPMTNVEREQHLQGARKKAADAAREDEINDSEFRAIL
                     RDGQKLLRMSEEEFADELRVSHPRLNRWLHGKDLPHPVMRPGIAAWVAHKLSASVGNK
                     "
     gene            916..1443
                     /locus_tag="VN178_04580"
     CDS             916..1443
                     /locus_tag="VN178_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80279.1"
                     /translation="MFGVRRKNTNEFVGLCYVVQDSAESNWQLGGLKVTIQKEGIGEL
                     LVRFALAHTIVYGVLLQEGKWVQKIMAYVRNDNHAPLRLLTSLGFEHSGIDVFSHEDA
                     PAWMKRNRERGDFVFDEFTFTLDGLRQLAAWFSERFDGALHPSGAEAQIDLAGHNRME
                     DLTRALRHLAGELDP"
     gene            1613..1993
                     /locus_tag="VN178_04585"
     CDS             1613..1993
                     /locus_tag="VN178_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80280.1"
                     /translation="MMDHGYLRYLPGDQVRLNMEIIKYARMHVREAGVVFRHVEHDQS
                     ELTASGPPETIGGQSNVARLTLPIPAVATPGLYRVNRMWVETYGGRRYDYEGEEVPEH
                     ASTLAFEVEEEPDAKPQLSLSYHL"
     gene            complement(2078..2182)
                     /locus_tag="VN178_04590"
                     /pseudo
     CDS             complement(2078..2182)
                     /locus_tag="VN178_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2370..2975)
                     /locus_tag="VN178_04595"
     CDS             complement(2370..2975)
                     /locus_tag="VN178_04595"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWS80281.1"
                     /translation="MSGGSVASRDRCAWVGADPLYVAYHDEEWGVPVHDDQRLFEMLV
                     LEGAQAGLSWLTILKKRGGYRRAFDGFDPEKVARYDERKVEELLADPGIVRNRRKVEA
                     TVRNARSFLAVREEFGTFDRYLWGFVGGEPAHNRWRTPEEVPAETEASRVLSKDLKRR
                     GFGFVGPTVVYAFMQAVGMVNDHTTDCFRHAQLVGSTDHRP"
     gene            complement(2972..3838)
                     /locus_tag="VN178_04600"
     CDS             complement(2972..3838)
                     /locus_tag="VN178_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWS80282.1"
                     /translation="MVAESVAEGASDLDRLARDAAGGDKRALGELCRELQHPIYRLAL
                     RMLSSPEDAEDATQEVLVRIVTNLGTFEGRSRFMTWVYTVATRQLLRTRKRLVESSVR
                     GADAFAAALDAGLGPVPDDVEGQAEYEELCAEVRISCTYGMLLCLSREVRLAYILGDL
                     MGMTDRDGAEVCEVTPAAFRKRLSRARKTMRRIIANRCGLVREENPCRCGGQIASGER
                     LGVLERGNLKLATHPRGDGRPILADTTIEAAARQLDLAEAIAEVYKTDPEFVAPRRVY
                     ERLRQAAPDLLV"
     gene            complement(3843..4148)
                     /locus_tag="VN178_04605"
     CDS             complement(3843..4148)
                     /locus_tag="VN178_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWS80283.1"
                     /translation="MAQETIEMVRFTVRPGAEEALVEERPAMVEAVRAEFPGMLSARL
                     AKLDETTWLELLVWTSREAAQRATEKAHDIPAVAAWLSHIAEVIGIERADVIVAEGR"
     gene            4379..5527
                     /locus_tag="VN178_04610"
     CDS             4379..5527
                     /locus_tag="VN178_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWS80284.1"
                     /translation="MKIVDVVPHVLSTPLEDPFSFSQGWVHRRSTMIVEVSTDEGVTG
                     WGESLCHGLQPPQVAASFVEFCFKPMLVGRDPFDVEVLWEEMYNRTRPFGGGAAVNAM
                     SGVDIALWDVIGRYLERPIHKLLGGAFRSKVTPYATGFYRREGQNHVEAGVEEARRHV
                     SEGFGAMKLKVGFGVEADVEYVHAIREAVGSGVRLMMDANCAYDVPAARRLLLGCRAA
                     EVHFLEEPLAPEDLEGYKALRNLSATYVAAGENLFGKIGYRRWISEGALDILQPDLCS
                     SGGFTECKKIAALAQAWNTAVVPHVWGSGIGLAASLQLIATLPPTPLSLQPEEPMLEY
                     DRSSHPFREDLIGGAISTVDGKVPVPHGPGIGVEVDRNVLERYGKPFR"
BASE COUNT         1052 a         1815 c         1810 g         1036 t
ORIGIN      
        1 cgagcacggc ttcttcatag ccgccggcga cttctacgcc tcgacgctcg ccgaaaagct
       61 cggcatcggg gacagtggag ggttcatccg cgccggcctc gccccttaca acaccgaaga
      121 cgaggtcgag aagttcgtcc aggcgttgga ggggatcata cgggaaacct gagttccaac
      181 gtcgcttggc gcggtaccgt cctacgagga ccacccggtg atgcgcttcg tggaagccgg
      241 cgtcccggtc gccctctgca ccgaagatcc cgtccaggtc tgcaccaagc tcggacatga
      301 gtacgctctg gccgccgcgc tcggcttctc ggtgaaggag cgttcgttgt aaaatgtgcg
      361 gtgggactcg tcagtcccac cctctatagc ggaggcgggc gccatgagca agcaaacgcg
      421 cgagcgtgcc taggacgaac tggctagagc actggccagc ggccgcatat cgcgaggtgt
      481 aggagcggct ccaacagaac gctgggaaaa gggagccgag taggggaggt aatcaatgtc
      541 tgaaccaata cctggtactc caatgacaaa cgtcgaaaga gaacaacatt tacaaggggc
      601 acggaagaaa gctgcagatg ctgcaagaga agacgagatc aatgactcgg aatttcgagc
      661 cattcttcgt gatggacaga agctgcttag aatgtctgag gaagagtttg ctgacgaact
      721 ccgcgtgagt catccaaggc tcaatcggtg gcttcatggc aaagatcttc ctcatcctgt
      781 gatgagaccg ggaattgccg cgtgggtagc ccacaaactc tcagcaagcg tagggaacaa
      841 ataggcaagc gctgctcgtg tcgcatccac cctcggatag agtccgagat agccgagttt
      901 gccaaaagcg gacacttgtt cggtgttcgt cggaagaata ccaacgaatt cgtcggccta
      961 tgctacgtag tgcaagattc agctgaaagt aattggcagc tgggtggcct gaaggtcacc
     1021 atccaaaaag agggaatcgg agagcttctg gtgcgctttg cgctggctca cacgatcgtg
     1081 tacggggtcc tactgcagga aggcaagtgg gttcagaaga tcatggcata cgtccgcaat
     1141 gacaatcacg ctcctctaag gctcttgacg agccttggtt tcgagcactc gggaatagat
     1201 gtgttctccc atgaggacgc tcctgcatgg atgaagcgaa accgtgaacg gggagacttc
     1261 gtcttcgatg aatttacgtt cacactcgat gggcttcgcc aattggcagc gtggttcagc
     1321 gaaaggtttg atggtgcgct tcaccccagc ggtgcggagg cacagatcga cctcgccggc
     1381 cacaaccgga tggaagacct cacgagggca ctccggcacc tcgcaggcga actcgatccg
     1441 tagctatgcc ttctgggatc gtatgctgga tgctgcatag atcgctgctg agggccgcga
     1501 ggatggcctc tgaggggctt ggcgggtccg catggacgca gaaaaactgg ggcaatagcg
     1561 cctttgagcg cgggaaggta ggatgagtct ctaagcgacg agggaggcgc cgatgatgga
     1621 tcacggctac ctgcggtact tgccggggga ccaggtcagg ctgaatatgg agatcatcaa
     1681 gtatgcgcga atgcacgtgc gcgaagcggg cgtggtgttc cgccacgtgg agcatgacca
     1741 gagcgaattg actgccagcg ggccgccgga gaccatcggg gggcagtcca acgtcgcgcg
     1801 gctaacactc cccatacccg ctgtagccac gcctggcctc tatagggtga accggatgtg
     1861 ggtggagacc tacgggggac gccgctacga ctacgagggg gaagaggtgc cagaacacgc
     1921 cagcacgctc gccttcgagg tggaggagga gccggacgcg aagccgcagc tctcgctcag
     1981 ctaccacctg tagcaagttc cccttattga ccaagcttcc tgtaagcggc attaccggaa
     2041 gttcgctcgc aatgctctcg aaactgtctg aaaaccctca tgagcgggcc aatctcctcg
     2101 ccatcagacg actcatagca gcgtagataa aggcttctgc gctctccggc agacgctcgt
     2161 agtctttgct catcctcctg tggctaccgc gctccttagg gcggccgatg gccgttgttg
     2221 cttcagaccc gagccaaccc cggtaggcgt ccccggttac atccttgacg gtgatgcgct
     2281 tctcggagcc tgtgccctct acctgcgaaa tctcgacgcc taactcctgg accctgtgcc
     2341 aggcggccta ctcagcgtcg ggcccggcgt tagggccggt ggtcggtgct ccctaccaac
     2401 tgcgcgtggc ggaagcagtc cgtcgtgtgg tcgttgacca tgcccaccgc ctgcatgaac
     2461 gcgtagacca cggtgggacc gacgaacccg aacccccgac gtttcaggtc cttgctcagg
     2521 accctggatg cctcggtctc ggcgggaacc tcctccggtg tccgccagcg gttgtgcgcc
     2581 ggctcgccgc cgacgaagcc ccagaggtat cggtcgaagg tcccgaattc ttcccgaacc
     2641 gcgaggaagg acctggcgtt gcggaccgtc gcctccacct tccggcggtt gcggactatg
     2701 ccgggatcgg ccagcagttc ctcgaccttc cgttcgtcgt accgggcgac cttctccgga
     2761 tcgaagccgt cgaaggcacg ccggtagccc ccgcgcttct tgagaatcgt gagccaggag
     2821 aggcccgcct gcgccccttc cagcacgagc atctcgaaga gcctctggtc gtcgtggacc
     2881 gggacgcccc actcctcgtc gtggtaggcg acgtagagcg ggtccgcgcc gacccaggcg
     2941 cagcgatccc tcgacgcaac gcttccgccg ctcataccag gagatcggga gctgcctgcc
     3001 gcagccgctc gtagaccctt cggggggcga cgaactcagg atccgtcttg tagacctcgg
     3061 cgatcgcctc ggcgaggtcg agctgcctgg ccgccgcctc tattgtggtg tcggccagga
     3121 tcgggcgccc gtcgccacgc gggtgggtcg cgagtttgag gttaccccgc tccaggacgc
     3181 ccagccgctc gccgctggct atctggccgc cgcaccggca cgggttctcc tcgcgcacca
     3241 ggccgcagcg gttggcgatt atcctcctca tggtcttgcg ggcgcgtgag aggcgcttgc
     3301 ggaaggcggc gggggtcacc tcgcagacct cggccccgtc gcggtcggtc atgcccataa
     3361 ggtcgccgag gatgtaggcg agccgcacct cccgcgagag gcacaagagc atcccgtagg
     3421 tgcaggagat tctcacctcg gcgcagagct cctcgtactc cgcctgcccc tcgacgtcgt
     3481 cgggcaccgg ccccaggccg gcgtccaggg cggcggcgaa ggcgtccgcg cccctcaccg
     3541 acgactccac gaggcgcttc ctggtccgca agagctggcg ggtcgccacc gtgtagaccc
     3601 aggtcatgaa cctcgagcgg ccctcgaagg tgcccaggtt ggtgactatg cggaccagga
     3661 cctcctgggt ggcgtcctcg gcatcctcgg ggctggagag catacgcagg gcgagccggt
     3721 agatggggtg ctgcagctcc cggcacagct cccccagggc gcgcttgtct ccccctgcgg
     3781 catcccgggc cagccggtcg aggtcggacg cgccttcggc cacgctttcg gccaccacgt
     3841 cgctagcgcc cttccgcgac gatcacgtcg gcgcgctcga tgccgatcac ctccgctatg
     3901 tgcgagagcc aggccgcgac ggcggggatg tcgtgcgctt tttcggtcgc cctctgggcc
     3961 gcctcccggc tcgtccacac gaggagctcg agccacgtcg tctcgtcgag cttcgctagc
     4021 cgggcgctca acatgcccgg gaactcggct ctgaccgctt ccaccatcgc cggacgctct
     4081 tctacgagcg cctcctccgc gccgggcctg accgtgaacc ggaccatctc gatggtctcc
     4141 tgcgccatgc tcgctccttc cgtaaatgtt ccgtcctata gttagagaca tcccgccccg
     4201 ggagcgtgac atcgcgctcg caatgcacca cgattgacgg gacgttttcc cgtagcctga
     4261 agcccactat ccaaagtgta cggtctttct gcaaaccact cctatcgccg aataccggtg
     4321 aagcaggtag ccttacgcgg gtgagccggg ctcgagatct gacgaggtgg caagaaacat
     4381 gaagatagtc gacgtagttc cccatgtact gtccacgccg ctggaggacc ccttttcctt
     4441 ctcgcaaggt tgggtccaca ggcggagcac gatgatagtg gaggtctcta ccgacgaggg
     4501 cgtcacaggc tggggcgagt cattgtgcca tgggctgcag ccaccgcagg tcgccgcctc
     4561 cttcgtcgag ttctgcttca agcccatgct cgtcggccga gatcccttcg acgtcgaggt
     4621 actctgggaa gagatgtaca accggacccg tcccttcgga gggggcgccg ccgtcaacgc
     4681 tatgagtggt gtagacatcg ccctctggga cgtcatcggc cgctacctgg aacgtccgat
     4741 ccacaagctg ctgggcggtg cattccgctc caaggtgacg ccctacgcga ccggctttta
     4801 ccgccgtgaa ggtcagaacc acgtggaggc aggcgtcgag gaagccaggc ggcacgtgtc
     4861 cgaaggcttt ggggccatga agctaaaggt cggcttcggg gtagaggccg acgtcgagta
     4921 cgtccatgcc atcagggagg ccgtgggttc cggggtgcgc ctcatgatgg acgccaactg
     4981 cgcctacgac gtccccgcag cgcgccgcct cctacttggg tgccgggccg ccgaggtcca
     5041 cttcctcgag gagcccctgg cgccggagga cctggaaggg tacaaggctt tgaggaacct
     5101 gagtgccacc tacgtcgccg ccggggagaa cctgttcggc aagatcggct accggcgctg
     5161 gatctccgag ggcgccctgg acattctgca accggatctg tgctcctcag ggggctttac
     5221 cgagtgcaag aagatagcag ccctggccca ggcgtggaac acggcggtgg tcccgcacgt
     5281 ctggggctcg gggatcgggc tggcagcctc cttgcagctc atcgccacgc tcccgccgac
     5341 gccactgtcc ttgcagcccg aagagccgat gctcgagtac gaccggtcgt cgcacccttt
     5401 ccgggaggac ctcatcggcg gcgcgatctc caccgtggac gggaaggtac cggtgccgca
     5461 cggaccaggt atcggtgtcg aggtagatcg caacgtgctc gagcgttacg gcaagccgtt
     5521 ccgctagccg ctcatgcctc cgagcggctt gaccaacgtc agctcgcctg gaaagagatc
     5581 tgcttgcgga tgctcgagca cggcttcttc atagccgccg gcgacttcta cgcctcgacg
     5641 ctcgccgaaa agctcggcat cggggacagt ggagggttca tccgcgccgg cctcgcccct
     5701 tacaacaccg aag
//
LOCUS       DATMVF010000101         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_290083, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000101 DATMVF010000000
VERSION     DATMVF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290083"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..915)
                     /locus_tag="VN178_04615"
     CDS             complement(328..915)
                     /locus_tag="VN178_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HWS80285.1"
                     /translation="MEELARRSGGAFRAGDAVEIGVHPRDLYALRDEGFLVELSRGVY
                     RPADAEINAYLDLVAVSRRSPRGSICLNSALSFWDLTDEVPAEVHLAVPRGSHRPRIS
                     YPPTRVHVFAAYTFDLGRERVCLESGEEINIYSQERSVVDAVRLRGRVGTGVAYEALR
                     RYLRRPRSSPGELLRLARRLRAGGPMADALDVLTG"
     gene            1838..>3999
                     /gene="mbhE"
                     /locus_tag="VN178_04620"
     CDS             1838..>3999
                     /gene="mbhE"
                     /locus_tag="VN178_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HWS80286.1"
                     /translation="MDLAWAPSWDLRIALSLDGLAALYALLATGIGFLVLAYSSRYLP
                     LHLEHEGRPQTAAVRFYFFVLLFMGSMVGLAMAQDLILLFLFWDLTAVASYYLIGYDA
                     HKEESRASALMALLVTGVTAVLLLIGALLLYAAHDTFSIPELARRVGPGPLLTGSGLL
                     IVVAGLAKSAQVPLHFWLPRAMAAPTPVSAYLHSAAMVAAGVLLIGRVYPLLQDSDLI
                     LDTLLVVGIASMAVGGLLALTRDVLKQLLAYSTIAQYGFVVTMYGLGGEYGAGGAAYY
                     VIAHAIAKSALFLTAGAVTEATGQDHLSKLGGLLRPMPLLAVSSGLAAATLTSLPLTL
                     GFFADEFFFAAALERGLLFATTAVVSAAATLAYTWRFWGGIFLGEPHASSVAPRPVPR
                     LLVVPVVALGAIGLLGGLLAGPFERLAEAAGEVSFAAPTPLDATYHAEVLPEYLMALA
                     AYALGVAATASRSVWSRAALGFSDLGKLAGPERLYRLTIRGLNRLSDLIHRLEIRNLR
                     GRVAFVLLPTAVLVGAAVLATPTAGAYRIGEFRAGEAPLMLVMLAVVAAALTTTFTRR
                     HVTLVLVLSSAGFALAVAYAFYGAPNVALVAVLVETMLTLLVVATLKLIPYRILHRQA
                     ELPLTRRGRKVFVSVVAGAFAFVVVWGALSQPPAGTTVAEEHVRLAPQAHAKNVVTAT
                     LADFRGLDTLGEISVVAIVLLGVATLLGGVRPETLPTD"
BASE COUNT          601 a         1371 c         1261 g          766 t
ORIGIN      
        1 ctgatggtca tggccctcgc ctgcgagacg tcgaaggtca tgccatcgtc cacagggatg
       61 ctcgccacct cccggatcac ggccaacagg ctctcctcgt caccggcgac gccccacgcc
      121 agcacgtctg cgtcgcgcgt cggacgccca ggaccgtaag caggaggcca cccttgagca
      181 cgaagcgatc gcggtacggg gagactgaga gcctgtagag aaagcgttcg agcaggtaga
      241 actcgaagag ctcctgggtc cctcgcccct cgcgcctggc ccgccgctgt agggctcggt
      301 agacctcctg cggtgtcgtt ggctcgctca acccgtcagc acgtctagag cgtcggccat
      361 gggtcccccg gccctcagcc gccgagcgag acgcagcagt tcgcctggag aggacctggg
      421 gcgccgcagg taacgtcgga gggcctcgta tgcgacgccg gtccccaccc ggcccctgag
      481 gcgcacagca tccaccacgc tacgctcctg agagtagatg ttgatctcct cgccggactc
      541 caggcagacc ctctcacgcc ctaggtcgaa ggtgtaggca gcgaagacgt gaacacgcgt
      601 cggcgggtag gagatcctgg gccggtgcga tcccctaggc accgccaggt ggacctccgc
      661 aggaacctcg tccgtcaagt cccagaaaga gagcgccgag ttcaggcaga tggacccccg
      721 tgggctgcgc cgcgagacgg ccaccaggtc caggtaagcg tttatctcgg cgtccgctgg
      781 gcggtagacc ccacgcgaaa gctcgaccag gaatccctcg tcccgcaggg catacaggtc
      841 acgcggatgg acaccgatct ctaccgcgtc tcccgccctg aaggctcccc ccgaacgccg
      901 ggcaagctcc tccaatgtac gtcgctgttc taacattcgg ataaaagtct acacaagttg
      961 tcgctatatg tgtagatttt tatacattac actgaggaca aggttattgt ggaacaccca
     1021 caccgggttc tgtccgcaag ccgttggcgt tgtgaatcga agatagcccg ttccagaccg
     1081 gcctacttcc gatacttggt aggatctggc acgccagcga gctggaaagc ctccttgcgc
     1141 tcgttgcagg ttccacacag accgcagtgg atctcgccac cctcataaca actccaggtg
     1201 tcttcgtagg ggacacctag agagatccat cgcgttgatg gcccaggcgg gataggtgta
     1261 gtcggcactc ggcgacgaca ctctggtgcg gcatgagaag tcacgcgatc tccctctctg
     1321 gcgatcgggt tcgctcttgc tttgcgtcat ctctagcttc gaatctgcct accgcgctcg
     1381 ctaaagtcta gcttctccgc tgaccttcgg ctgtcctgcg ccccgctgtg ctacaggttg
     1441 cggcgttgcc aacagggctg catccagggt cgcaggcgta ggtcgcttgc gacgggggga
     1501 tatatctccc gagatggcat gcaagaatca tttctggctc cccggcaggg gagagcagca
     1561 acctcctgcg cgtgccgacg gccgggttag gaacgggaag tccgcggaca ccttgatcca
     1621 gacacgaaga tatcgtttac agggtcaccg ccaggagggg agatagaacg gtgttgacga
     1681 tgctcgccgt tctcctgccg atggcgctcg ccctcctcgc ggcccagtcg cggccggggt
     1741 aggcgcgttc agggccgctt acgcggcccc ggtcggggcg gccttcgccg ccctcgccgc
     1801 cctctcggtg ctttgggggt ggttcgccgg cggcagcgtg gatctggcct gggcaccgag
     1861 ctgggatttg cgcatcgccc tctcgctcga cgggctcgcc gccctgtacg ccttgctcgc
     1921 gaccggcata ggcttcctcg tcctcgcata ctcctcgcgc tacctgccgt tgcacctgga
     1981 gcacgagggt cgcccccaga ccgccgccgt acgcttctac ttctttgttc ttctgttcat
     2041 gggctctatg gtaggcctgg cgatggcgca agacctgatc ctcctcttcc tcttctggga
     2101 ccttacggcc gtcgcgtcct actacctgat cggctacgac gcccacaagg aagagtcgcg
     2161 ggcctcggcg ctgatggcgc tgctagtcac cggggttacg gcggtgttgc tcctcatcgg
     2221 cgccctcctc ctctacgccg cccacgacac cttctccatc ccggagcttg cccgacgtgt
     2281 cgggccggga ccgcttctca caggctcggg cctgctcatt gtagtggccg ggctcgccaa
     2341 aagcgcgcag gtccccctgc acttctggct gccgcgagcc atggccgccc cgacacccgt
     2401 ctccgcctat ttgcactcgg cggcgatggt ggccgcgggg gtgttgctga tcggacgtgt
     2461 ttacccgttg ctccaggata gcgaccttat cctcgacacg ctcctggtcg tcggcatagc
     2521 ctctatggcc gtcggcggcc tcctggcgct tacgcgggac gtcctcaagc agctcctcgc
     2581 ctactccacc atcgcccagt acggtttcgt ggtcaccatg tacggcctcg gaggagagta
     2641 cggcgccggg ggcgcggcct actacgtgat cgcccacgcc atcgccaaga gcgccctgtt
     2701 cctcaccgcc ggggctgtga cagaggccac cggccaggac caccttagca agctcggagg
     2761 attgctgagg cccatgccgc tgcttgcggt gtcgtcagga ctcgcggcgg ccacgctcac
     2821 ctcgctcccc ctgactttgg gattcttcgc cgacgagttc ttcttcgccg ccgcgctcga
     2881 acgcggtctc ctcttcgcca ccacagcagt cgtctcggcg gctgcgaccc tggcctacac
     2941 ctggcgcttc tggggcggca tcttcctcgg agagccccat gcctcctccg tcgcgccccg
     3001 ccccgtaccc cggctgctcg tggtgccggt cgtggcgctg ggagcgatag ggctgctcgg
     3061 gggtctgtta gcggggccct tcgagaggct ggccgaggcg gcgggtgagg tttccttcgc
     3121 ggctcctacg ccgttggatg ccacgtacca tgcggaggtt ctccccgaat atttgatggc
     3181 cctggcggcc tacgcactgg gcgtggcagc gacagcctcg cgctctgtat ggtcgcgggc
     3241 cgcgctcggg ttcagcgacc tcgggaagct ggcggggcct gagcggttgt accgtctgac
     3301 cattcgcggg ctcaaccggc tctcggatct catccaccgg ctggagatcc gcaacctgag
     3361 gggccgggtc gctttcgtgc tgttgccgac ggccgtgctc gtgggggcgg ccgtgctggc
     3421 aacgcccaca gccggcgcat accggatcgg agagttccgg gctggggaag ctccgctaat
     3481 gctggtgatg ttggcggtgg tggcggccgc cctgacgacg accttcacgc ggcggcacgt
     3541 taccctggtt ctcgtgctct ccagcgcggg cttcgcgttg gcggtcgctt atgcattcta
     3601 cggggcgccg aacgtggcgc tcgtcgcggt cctcgtagag accatgctca cgctgctcgt
     3661 cgtcgccacc ctcaagctga tcccttaccg catcctgcac cgccaggcgg agttaccact
     3721 tacgagaagg ggccgcaagg tcttcgtctc ggtagtcgct ggcgccttcg ccttcgtggt
     3781 ggtctgggga gccctctcgc agccgccggc cggtacgacc gtcgccgaag aacacgtacg
     3841 gctcgccccg caggcacacg ccaagaacgt ggtcacggcc acgctggcgg acttccgcgg
     3901 gctggacacc ctgggcgaga tctccgtcgt ggccatcgtc ctcctggggg tggcgaccct
     3961 gctcgggggg gtgaggccgg aaaccctccc gaccgatag
//
LOCUS       DATMVF010000102         4463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_290148, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000102 DATMVF010000000
VERSION     DATMVF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290148"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VN178_04625"
     CDS             complement(<1..623)
                     /locus_tag="VN178_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWS80287.1"
                     /translation="MHHVRAMDRPVSRSEMTDALGVSRSKISMEVGRLIEAGLLAEDG
                     LAKSEGGRRSSLLRIPRSAGLIAAVDLGATSTDVALATLGGELLVHRGERGDIKDGPT
                     KVLDRVKELLSELLSEQGADAQDVLAIGVGVPGPVEHASGVLRSPPIMPGWDGFPIRA
                     AFADEYEAPVFVDNDVNVMALGEHRDGLGKGENDVLFVKIGTGIGGG"
     gene            904..1311
                     /locus_tag="VN178_04630"
     CDS             904..1311
                     /locus_tag="VN178_04630"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80288.1"
                     /translation="MRIAFAESADLDHLARADHHLRRGVIEQKIERREIVVLHHDGRR
                     AGLLRWGYFWDEIPFMNLLWVREELRGRGFGTRLVGFWEDEMRKAGYEEVMTSTMSNE
                     RAQHLYRRLGYKDCGSLLLSDEPLEIILSKKLA"
     gene            complement(1355..2689)
                     /locus_tag="VN178_04635"
     CDS             complement(1355..2689)
                     /locus_tag="VN178_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HWS80289.1"
                     /translation="MPLDYTYRIQLPHRTGQLAKVAGTIAEGEGLIGEVNTISVGRVA
                     SVREITLEVRDKDHAENIEAMLEALPGVEVLWARDRALLRHEGGKLTIDSIRPVRTVQ
                     DMRDVYTPGVARACMAISEEPSLVGELTMIGRSVAICTNGTRVLGLGDIGCVASMPVM
                     EGKAVFYHQLSNVSAMPILVDTKDVDEFVETVVRISPTFGGIHLEDISAPECFEIEAR
                     LIEALDKPVMHDDVHGTAVVTLAAVLVACRHAGLTLQDEVVGQIGLGAAGFGIAALLV
                     DAGARRVLGSDPNPLSHERAQSKGIEITDLETVMREADVVVATTGRSGLIDPAMVREG
                     QVILALTNPYPEIDADEAVEAGAAFAADGTSVNNVLGYPGIFRGALLSGAREINLPMK
                     LAAAEKIASLASGSELVPDALDSQVHEQVADAVRDAAVRSGAAHLERAPVGL"
     gene            complement(2774..3172)
                     /locus_tag="VN178_04640"
     CDS             complement(2774..3172)
                     /locus_tag="VN178_04640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWS80290.1"
                     /translation="MSRRVITSPSLPAPMRGGAFSSGVEAPAGRTIYVSGQVSMDAEG
                     NVVGEGDVRLQTQTVLEHVKTVVEEAGGGMNDIVKVTVFIRDMRHYDEIHEVRRRYFE
                     EPFPASSMVEVSALIDPRLLIEIEAVAVVG"
     gene            3284..>4463
                     /locus_tag="VN178_04645"
     CDS             3284..>4463
                     /locus_tag="VN178_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HWS80291.1"
                     /translation="MQRTFDVEVVREQFPALGRTHNGRGVVYFDGPGGSQVARQAIEA
                     ITGYMERGGANLHGVFPTSTETEEILSDTREAAADFFGTAPDEVAFGANMTTLTFAVS
                     RALARGWDRDSEIVVAELDHRANVDPWLIAAEEQGVRVRWVRVDPETLTLDGNDLEHK
                     INERTELVAIGLASNAVGTVNDVAAVAERVHSRGALVAVDAVHAAPHIPIDRDAMGAD
                     IITCSAYKFFGPHVGVTAIRRALFETLGVYRLDPAPDYIPDKLETGTQNHEGIAGVKG
                     ALDFISSLGEGASQRERLTSAMNAIEEHEAALAETFRTALRDIPRVQFYTAPDGVRKT
                     PTIAFRVEGHTPEEICLRMLEHGFFIAAGDFYASTLAEKLGIGDSGGFIRAGLAPYNT
                     E"
BASE COUNT          842 a         1462 c         1358 g          801 t
ORIGIN      
        1 atgccaccgc cgatgcctgt gccgatcttg acgaagagca cgtcgttttc gcctttgcca
       61 aggccatccc tgtgctcgcc cagcgccatc acgttcacgt cgttgtccac gaagaccggg
      121 gcctcgtact cgtcagcgaa cgccgcacgt atgggaaacc cgtcccaacc aggcatgatc
      181 ggcgggctcc tcaagacccc cgaggcgtgc tccacaggcc caggcacccc gacacctata
      241 gccaggacgt cctgcgcatc agccccctgc tccgagagta gctccgagag caattccttg
      301 accctgtcga gcaccttcgt tggcccgtcc ttgatgtccc cgcgctcccc gcggtgtacc
      361 aggagctcgc cgcccagggt agccagggcg acgtccgtgg aagtggcgcc gaggtcgacg
      421 gcggctatca ggccggcgga acgggggatg cgcaacagtg aggagcgccg accaccctcc
      481 gacttggcca ggccgtcctc ggcgagcagg ccggcctcga tcaaccgccc cacctccatg
      541 gagatcttgc tcctgctcac acccaacgca tccgtcatct cggatcgcga tacaggacga
      601 tccatcgccc tgacgtgatg catgatctcc gcctgaatgc ggtcctcgac aagcggatgc
      661 cagccaccgg ctgcctcgtc tacatggtcc ttactcctca caaacacagt atagagaaac
      721 ttcctccatg cgtccaacga cttttgacga tctttggcat aagtcacgga ataggcgcgg
      781 aaagtcgaag cgcgttggga cacgttttac atgttcgtga agtacgcgac gtacgccctc
      841 ccggcgccgg atgagacaat gctgcccgta ttgtccggtt cgttgtagaa gaggagcatc
      901 aacatgcgca tagccttcgc tgagtccgca gacctggatc atctggcgcg agcggatcat
      961 cacttgcggc gaggtgtcat cgaacagaag atagaacggc gcgagatcgt ggtgctacat
     1021 cacgacggtc ggcgggcagg actcttgcgc tggggatact tctgggatga gatccccttc
     1081 atgaacctgc tgtgggtccg ggaagagttg cgggggagag gattcggcac caggctcgta
     1141 gggttctggg aagacgagat gcgaaaggcg ggctacgaag aggtcatgac ttccactatg
     1201 tccaacgagc gggcgcagca tctgtatcgc agactcggtt acaaagactg tggttcattg
     1261 ctcctgagtg acgagccttt ggagatcata ctctcaaaga agctggcgta aagaagacgg
     1321 cggcgatctc acccatctgg tcaggctcgc tccactacaa tcccacaggc gccctttcga
     1381 ggtgggcggc gccgctccgg accgcggcgt ctctgacggc gtctgcgacc tgttcgtgca
     1441 cctgggagtc gagggcgtcg gggacgagtt ccgaccctga ggcgagggag gctatcttct
     1501 ctgcggcggc gagcttcatg gggaggttga tctccctggc tcccgagagg agggcgccgc
     1561 ggaagatgcc ggggtagccg agcacgttgt tcacgctcgt tccgtctgcg gcgaaggcgg
     1621 cgcccgcttc gacggcctcg tcggcatcga tctccgggta ggggttggtg agggccagta
     1681 tcacctgccc ttcgcgcacc atcgctgggt ctatgagacc ggatctaccc gtcgtggcca
     1741 ctacgacgtc ggcctcccgc atgacggtct cgaggtcggt aatctctatg cccttgctct
     1801 gggcgcgttc gtgggagaga gggttggggt ccgagccgag gacgcgcctc gcgccggcat
     1861 cgaccagaag ggcggcgata ccgaatccgg cggcgccgag cccgatctgg cccaccacct
     1921 catcctggag ggtcagccct gcgtggcgac aggcgacgag cacggccgcg agcgtcacga
     1981 cggccgtacc atgaacgtcg tcgtgcatca cgggtttgtc gagggcctct ataaggcggg
     2041 cttctatctc gaagcactca ggggccgaga tgtcctccag gtggatgccg ccgaacgtag
     2101 gcgatatgcg cacgacggtc tccacgaact cgtcgacatc tttcgtatcg accaggatcg
     2161 gcatagcaga gacgttggag agctggtggt agaagacggc cttcccctcc atcacgggca
     2221 tcgaggcgac gcaaccgata tcgccgagcc ctagaacccg cgtgccgttc gtgcagatgg
     2281 cgacgctgcg ccctatcatg gtcagctcgc cgacgagcga aggttcctcg gagatggcca
     2341 tacacgcccg ggctaccccg ggggtgtaga cgtcgcgcat gtcctgcacc gtcctgacgg
     2401 gcctgatcga atcgatggta agcttcccgc cttcgtgtcg tagcagcgcc cggtcacgcg
     2461 cccaaaggac ttcgacgccg ggaagcgcct cgagcatcgc ttctatgttc tcggcgtggt
     2521 ccttgtcacg gacttctagt gtgatctcgc gcaccgaggc gacacgcccg acgctaatgg
     2581 tgttgacctc gccgatcaga ccctctcctt ctgctatcgt cccggcgacc ttggccaact
     2641 gccccgtgcg gtgcggcaac tgaatgcgat acgtgtaatc gagcggcaaa gtagaccccc
     2701 ttattcccag accaaatccc ttacccagat tctatattgg gccacacccg gcggccatgt
     2761 tgcacaatac cctctacccg accaccgcta cggcttcgat ctcgatgagg agcctggggt
     2821 ctatgagggc tgagacctcg accatcgagg acgctggaaa aggctcttcg aagtacctcc
     2881 tcctgacctc gtggatctca tcgtagtgcc gcatgtccct tatgaagacg gttaccttga
     2941 ctatgtcgtt catcccgcca ccggcctcct cgaccacggt cttcacgtgc tcgagcaccg
     3001 tctgcgtctg gagccttacg tcgccctctc cgaccacgtt tccttcggcg tccatcgaga
     3061 cctgaccgga aacatagatc gtacgccccg ccggggcctc gaccccggag gagaacgcgc
     3121 ccccacgcat gggcgccggc aggctgggac tggtgatcac acgcctcgac aaaacgacct
     3181 ccttgttgct gttcctctgc agggagtaag gtagcatccc cccagggccc gcgcccgcct
     3241 gatgtaaact gcgccgggca agcggatcgg agcgggagga tttatgcagc gtacgttcga
     3301 cgtagaggtc gtccgcgagc agttccctgc gctcggccgg acgcataacg gtcgcggcgt
     3361 cgtctacttc gatgggcctg gcggctcgca ggtggcgcgg caggctatcg aggccatcac
     3421 gggctacatg gagcgcggcg gtgcgaacct gcacggcgtc ttcccgacga gcacggagac
     3481 cgaggagatc ctttccgata ctcgagaggc ggcggcggat tttttcggga ccgcgcccga
     3541 cgaggtcgcc ttcggggcca acatgaccac cctgaccttc gcggtctccc gcgccctcgc
     3601 ccgcgggtgg gacagagatt cggagatagt cgtcgcagag ctcgaccacc gcgccaacgt
     3661 ggacccctgg ctcatcgccg ccgaggagca aggggtaaga gtgcgctggg tcagggtaga
     3721 tcccgaaacc ctcaccctcg acgggaacga cctggagcac aaaatcaacg aaaggacgga
     3781 actcgtagcc atcggcctgg cctcgaacgc cgtcggcacc gtgaacgacg tggccgccgt
     3841 cgccgagaga gtccacagca gaggcgccct cgtagccgtc gacgccgtgc acgccgcccc
     3901 gcacatcccc atagaccgcg acgccatggg ggccgacatc atcacctgct cggcctacaa
     3961 gttcttcggc ccgcacgtcg gtgtgacggc cataaggcgc gcccttttcg agacgttggg
     4021 cgtctacagg ctcgatcccg cgccggacta tatcccggac aaactggaga cgggtaccca
     4081 gaatcacgag gggatcgccg gcgtaaaagg cgcccttgac ttcatctcgt ccctgggtga
     4141 gggcgcctcg caacgcgaga gactcaccag cgccatgaac gcaatcgaag aacacgaagc
     4201 cgcactcgcc gaaacattcc gtacagcact ccgagacatc cccagagtac agttctacac
     4261 cgccccagat ggcgtccgca agaccccgac catcgccttt agggtggagg gtcatacgcc
     4321 cgaagagatc tgcttgcgga tgctcgagca cggcttcttc atagccgccg gcgacttcta
     4381 cgcctcgacg ctcgccgaaa agctcggcat cggggacagt ggagggttca tccgcgccgg
     4441 cctcgcccct tacaacaccg aag
//
LOCUS       DATMVF010000103         9140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_338378, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000103 DATMVF010000000
VERSION     DATMVF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338378"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VN178_04650"
     CDS             complement(<1..765)
                     /locus_tag="VN178_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS80292.1"
                     /translation="MEYRRLGRSGLFVSALTLGTMTFGGQGGFSKVGATNVAGARRQV
                     DMCLDAGINLFDTANIYSGGESEEILGEAISGRRDDLLLATKVRMPVADGPNDTGLSR
                     HHIIRQCEASLRRLGTDYIDLYQVHEWDGLTPLEETLEALDTLVKSGKIRYVGSSNYS
                     GWQLMKALGTSERLGLQRYISQQIHYTLQAREAEYELVPLAIDQECPILVWSPLAGGL
                     LSGKYRRDSAASEGRHVEGWDEPPVYDTDKLYDIVDV"
     gene            complement(1085..1246)
                     /locus_tag="VN178_04655"
     CDS             complement(1085..1246)
                     /locus_tag="VN178_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80293.1"
                     /translation="MDGGKESFVLIVLSNYRVEERVPYLGETRARIGDATWKGLDRAT
                     VEALVGGKV"
     gene            1547..2161
                     /locus_tag="VN178_04660"
                     /pseudo
     CDS             1547..2161
                     /locus_tag="VN178_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2203..2955
                     /locus_tag="VN178_04665"
     CDS             2203..2955
                     /locus_tag="VN178_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWS80294.1"
                     /translation="MYGENAPPSWARLPGARCRAVEHLTKLDGLKEFELFEGLEEGFF
                     QDLAIAAEVLELGADTALYREGEPSGAVYFVGEGRVKLYRTSGGPKARDQILGVVGEG
                     SVLGLGAAIEGRTQTQGATALTDCVLYAVFRDDLFEIMGRFPDGAVRLSRALAARTRE
                     LEDLVGDLVFRSAPQRVARMLLNLATEEGRVTKRGVVFAPSLSRQEMAEATGISREAL
                     SRALSRLAKEGILDLGSKTITILMPAELRART"
     gene            3105..3338
                     /locus_tag="VN178_04670"
     CDS             3105..3338
                     /locus_tag="VN178_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWS80295.1"
                     /translation="MTYVITEPCIGTKDQSCVEVCPVDCIYDAGDHFMINPEECIDCG
                     ACEPECPVEAIFPEDEVPDDMESYTTKAADYFE"
     gene            3438..4028
                     /locus_tag="VN178_04675"
     CDS             3438..4028
                     /locus_tag="VN178_04675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWS80296.1"
                     /translation="MLPANRFILSRMDDLGWRTLGRDHLYRNPWCAFRVDEVELPGGA
                     RIEYGVLESGGYAAMLPVTDRGDVVLVRQWRQPLGAFTLELPSGGVDAGEKPEVAARR
                     ELFEETGYRAEGLEHLVSVHTSPGRSTEVCHLFKCAAVRDERGPRPEPTEFIRVVELP
                     LGEALRMVSEGGISDAATVLGLLWGTAGGASAGGLH"
     regulatory      4034..4135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4173..4730
                     /gene="thiT"
                     /locus_tag="VN178_04680"
     CDS             4173..4730
                     /gene="thiT"
                     /locus_tag="VN178_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HWS80297.1"
                     /translation="MNAFRDTRVLTEAALAVALSFVLGLIVLFKMPYGGSVSLEMIPL
                     ILLALRQGWKVGVVAGAIYGLLDLTIDLYVVHPVQFILDYPLAFGALGLAGLFKPTVR
                     GAILGTTVAVLARFACHFLSGVVFFASVAPEGWNPYIYSAAYNAAYLAPSLGIAIVVT
                     VVLLKALERAQPSPRQLYYQGSKTP"
     gene            4732..5412
                     /locus_tag="VN178_04685"
     CDS             4732..5412
                     /locus_tag="VN178_04685"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HWS80298.1"
                     /translation="MRAAIFLNGSPESSDLLGRVAGCADLIVAADGGARYALEAGVVP
                     HLVVGDMDSLGEDLALEVERLGAALERHPVRKDKMDGQLAVLAARERGATAADLLCAG
                     GGRLDALFAVPHILLAAERIGLRSTVVADRVRMFVVEAGYRTVEGVPREIISIFPLSG
                     PAAGVTLEGMEYPLQNASLEPGDTLGFHNELIGSEATVSVGKGAVLVVHETENPVTGH
                     TDSRALTS"
     gene            5427..6194
                     /locus_tag="VN178_04690"
     CDS             5427..6194
                     /locus_tag="VN178_04690"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HWS80299.1"
                     /translation="MPGAFRPDTVLDRVEHRPYPVPSGPWALGMSWRDLLFMHWPVGV
                     EDLRPLVPPALGIDTFEGSAWLGVVPFDMTGVRPHFLPAVPGLSHFPEINLRTYVTAE
                     GRPGIWFFSLDARSRVAVRLARATFHLPYFDAEMSCRASGDEVYYRSVRTHRGAPPAE
                     FAARYSPVGETFESERGSIEDFLTERYCLYSVDGRGNVRRGEVHHHLWPLQPAEAEVG
                     TLAMTQQIGLKLPQTAPILHFSKRLDVLAWPPRRVAS"
     gene            6311..7183
                     /locus_tag="VN178_04695"
     CDS             6311..7183
                     /locus_tag="VN178_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWS80300.1"
                     /translation="MKADLVSGLLPVREDGRMLLLQRPTGTWEPPAGRLQPGESFEEG
                     AVRELYEETGMLVSPQRIIATWVGEAPSGRRLASVTYAGKSDVAGVRLSEEHLDHRWV
                     TLGEWMSLPSWWSHENIRRIARPVGAVREPPVPRKPPPPPLRGDTGVVPANLGAGAVL
                     VDLGGIEPRALLLRRRKPPVGLWENPGGMLEEGENFEVCASRELYEETGVLARIEDPW
                     WARVEPWRNPEDPELYAGVGFLARHKGGEIDPESAAHDASQWVTETGWRSLRTWYTKR
                     ESDVLWSAIREMQR"
     gene            7207..7479
                     /locus_tag="VN178_04700"
     CDS             7207..7479
                     /locus_tag="VN178_04700"
                     /inference="COORDINATES: protein motif:HMM:NF033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HWS80301.1"
                     /translation="MGQEHGGSRGAGLMRLVGIVLLVAAVLFWVAAPTVLLTPLSTAQ
                     KAWTGSAFLILGEVAFWVAAVVLGREVFRRYRRYLDPRGWLGEKRH"
     gene            7476..8408
                     /gene="thiL"
                     /locus_tag="VN178_04705"
     CDS             7476..8408
                     /gene="thiL"
                     /locus_tag="VN178_04705"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWS80302.1"
                     /translation="MNEFEVLRRISALLPPAPPEVLVPLGDDCAVLEIGGSTWVAASD
                     MLVSGHHFKDWATPEDVGYKAVAVNASDVASMGGTPRFVLASGGAPGPETALRCSRGI
                     LEACEEFGIYPLGGDTTRADALTVDVAILGELATRPVLRSGASPGDLLAVTGELGASA
                     AGLLALERGMTGPERLIRRCLRPEPRLSAGRVAARLGATAMIDLSDGLASDVRRLCER
                     SGAGCMVDLDLLPIKDDTRELARSLERDPEILAATGGEDYELLVCVPEPVLDALASDL
                     EVPLTLIGQITRSDVVFTREGEPVEGLSGWNHFT"
     gene            complement(8415..8840)
                     /locus_tag="VN178_04710"
     CDS             complement(8415..8840)
                     /locus_tag="VN178_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWS80303.1"
                     /translation="MEPEDVDAELERSVPAELGTKHMEESVDREVLARLRRLQGEGDP
                     DIVAELTGMFLKDSRSRLEAVEEALQKGDAPAVERAAHALKGGSGSMGARGMSGLCAQ
                     LEELGASVDLSRAPELLGRIREELGRVERALEAEVSGDR"
BASE COUNT         1626 a         2895 c         2963 g         1656 t
ORIGIN      
        1 cacatctacg atgtcgtaga gcttgtcggt gtcgtagacg ggaggctcgt cccagccctc
       61 cacgtgtcgt ccctccgagg cggcgctgtc gcgccgatac tttccagata gcaggccacc
      121 ggccagcggg ctccacacga ggatggggca ctcctggtcg atggcgagcg gcaccagctc
      181 gtactcggcc tccctggcct gcagcgtgta gtggatctgc tggcttatgt agcgctgcag
      241 cccgagcctc tcggaggtgc caagcgcctt catcagctgc cagcccgagt agttggatga
      301 tcctacgtag cgaatcttgc cgctcttgac gagtgtgtcc agtgcctcca gtgtctcctc
      361 gagcggggtg aggccgtccc actcgtggac ctggtagagg tcgatgtagt cggtgccgag
      421 ccttctcagg cttgcctcgc actggcggat tatgtggtgt cgtgagagac cggtatcgtt
      481 gggaccgtct gccacgggca tgcgtacctt cgtcgcgagc agtaggtcgt cccggcggcc
      541 ggagatagcc tctccgagta tctcctcgga ctcgcctccc gagtatatgt tcgcggtgtc
      601 gaagaggttg atccccgcgt ccagacacat gtccacctgc ctccttgccc cggcgacatt
      661 cgtggcgccg accttgctga agcccccttg acctccgaag gtcatcgtgc caagggtcaa
      721 ggcggagacg aacaaacccg agcgtccgag gcggcgatac tccataaggt cctccctacc
      781 tggttgagta aataaggtgc atgcgccagc ctagcacgag gagcactcca ttgtggtgcc
      841 gtagcccgcc tttcacagat tcaccgcttt cttcgcagtg agccgaatct cggcatcatt
      901 tagagccacc gtaactgccc ttcaaggacg ggaagcatgc agtgagggag aaggtatgct
      961 gcatgctcaa ggaatccttc aaacaactct tcgcgcgtac actgatcggc ccaactattc
     1021 tgccgctcct aggagagggg ttccggcgga aagaaactct aaccgatcag cggtactgct
     1081 cgcgttacac cttgcctccc actaaggcct ctacagtggc gcgatccagc cccttccaag
     1141 ttgcatctcc tatccgtgcc cttgtctctc caaggtaggg caccctctcc tccaccctgt
     1201 agttagagag gacaatcaac acgaagctct ccttaccgcc gtccattgcc tccttgggcc
     1261 agtgcttgac ccagactagt ggagtaggca gttcgttggc ccggatcacc cccgcggcta
     1321 tcaccttcgc agagttagtg atcgagcctc ccggattgtt aggtagctcc gagcagacaa
     1381 ccacgggagc gtcgagatcg ggcagagcct caaggacaac gcgcgcaagc cctcgaaggc
     1441 ggccagacgt ttctgggagc tctccggcgg catcctgcgt tgcgacgagt gcgggcacac
     1501 cttgagaccg aacagtgccc acaaatagaa gagcggcggg cggtttcact actacagctg
     1561 ccgctcccgc tacaacacgg ggccgagccg ggactgctct aacaggaaat tcctgcgggc
     1621 ggagcagatc gaggagcagg tgtgggggtt cgtgcgcggg ctgttgcgtg accccaagcg
     1681 gataagggcg ggaatcgacc ggctcataga ggaagagcgg accgacgccg gacgggaccc
     1741 ggggagggac gcggagttct ggtcgaggaa gatgaccgag gtcgaggtcg agcggcgcgg
     1801 ctatcacaga ctcaccgcga agggccacat gacggacgag gaactggtcg cggcgctctc
     1861 ggagctggac gaggttcggg agaccgccga gcgcgaacta gaatcggcac gggccagggg
     1921 cgaggtactt cggcgcctgg agcacgacag agacgccctg atgaggtcct acgcgggcat
     1981 ggtcagtgag accttggagg accttgcgcc agaagagcgc caccggatct acaagctgct
     2041 ccggcttggt gtccgcttcc gaccggactg gcccctggag atcacgggcg tcttcgcgca
     2101 ggtggcggaa gaagcggaga tcggttcgtc ggatagaaaa cctacgccac ttagcgtata
     2161 aagggtctgt gagatcgcgt gcgacgagag gaacaccaag cgatgtacgg tgagaatgcg
     2221 ccgccttcct gggcgcggtt gcccggcgcg cgttgccggg cggtcgagca cctgacgaag
     2281 ctggacgggc tcaaggagtt cgagcttttc gaggggctgg aagagggatt cttccaggac
     2341 ctcgcgatag cggccgaggt actcgagctc ggggccgaca ccgcccttta cagggagggc
     2401 gagccttctg gggccgtgta cttcgtcggc gaggggcggg tgaagctcta ccgcacatcg
     2461 ggcggcccga aggcgcggga tcagatcctc ggcgtggtcg gcgaaggctc cgtgctcggc
     2521 ctgggagcgg ccatcgaagg caggacccag acccagggcg ccaccgccct caccgactgc
     2581 gtgctctacg cggtcttccg cgacgacctg ttcgagatca tgggccgctt ccccgacggc
     2641 gccgtcaggc tctccagggc gctggcggcg aggacccgcg aactggaaga cctcgtcggc
     2701 gacctggtct tccgcagcgc cccacagagg gtcgcaagga tgctactgaa cctcgccaca
     2761 gaggagggcc gcgtaaccaa gaggggcgtc gtcttcgcgc cttctctcag caggcaggag
     2821 atggccgagg cgaccggcat ctcgcgtgag gccctctccc gcgccctctc ccggctcgcc
     2881 aaagagggca ttctcgacct cggcagcaag accataacca tactcatgcc cgccgagctc
     2941 cgcgcccgta cctgagaacg gctcgcaaca agcgcggttt ttgatcgagg tcacattacc
     3001 gtatgaccct aatcatgggc tgccatgacg ccggtcaacg gtgggtgttg tgggtggagg
     3061 ccggctggta taaagccggt acgagattca gagaggaggt ccttttgacg tatgtgatca
     3121 cagagccgtg tatcggcacg aaggaccaga gttgcgtcga ggtctgcccg gtggactgca
     3181 tctacgacgc cggcgaccac ttcatgatca accccgaaga gtgcatcgac tgcggcgcct
     3241 gcgaaccgga gtgcccggtc gaggccatct tccccgagga cgaagtcccc gacgacatgg
     3301 agagctacac caccaaggcc gccgactact tcgagtaggt tgcgaagggg tcttcgaccc
     3361 ctccgcaagc ggtcagcctt cagctatcag cttcttgttg taaatgaatg ctgaatgcgc
     3421 ggttggcagg aggcgctgtg cttcctgcca accgctttat actctcccgc atggacgact
     3481 taggctggcg gacgctcggg cgagaccatc tctaccgcaa cccgtggtgc gccttccgcg
     3541 tggacgaggt ggagttgccg ggcggggcgc ggatcgaata cggcgttctg gagagtggcg
     3601 ggtacgctgc aatgctaccc gtcacggaca ggggagacgt tgtgctcgtg cgccagtgga
     3661 ggcagccgct cggggccttc acgctggaac ttccgagcgg cggggtcgat gccggagaga
     3721 agcccgaggt ggcggcccgt agggagctct tcgaggagac gggttatagg gccgaggggt
     3781 tggagcacct ggtctccgta cataccagcc cggggcggag cacggaggtc tgccatcttt
     3841 tcaagtgtgc tgcggtgagg gacgagcgtg gtccgcgtcc cgagcctacg gagttcatta
     3901 gggtggtgga gttgccactt ggggaagctt tgcggatggt ctctgaaggt gggatctccg
     3961 acgccgccac cgtcctgggg ttgctctggg gtacggctgg tggcgcttcc gcgggtgggt
     4021 tacactaggg gcaacagcaa gaccggggag ccgtaaggct gagagggcac cgaagtgccg
     4081 acccgctgaa cctgatccgg gtaatgccgg cgcagggagg ttcaggagtc tcttattggc
     4141 gcatccccgt agaccggaag gaggatgcgc agttgaatgc cttcagagac accagggtac
     4201 tgaccgaggc ggcgctggcc gtcgccctgt ctttcgttct cgggctcatc gtgctcttca
     4261 agatgccgta tggcggctcc gtctccctgg agatgatccc cctcatactc ctcgccctca
     4321 ggcagggctg gaaggtgggc gtcgtcgcag gagcgattta cggtctgctg gatctcacca
     4381 tcgatcttta cgtagtgcat cctgtgcagt tcatcctcga ctacccactg gccttcggcg
     4441 ccctcggcct cgcgggactc ttcaagccaa cggtgcgggg tgccatcctc ggcaccacgg
     4501 tggccgttct ggcgcgcttc gcatgccact tcctctccgg cgtggtcttc ttcgcctccg
     4561 tcgcgcccga gggctggaac ccttacatct actcagcagc gtacaacgcc gcgtacctgg
     4621 ccccgagcct gggcatagcc atcgtggtca cggtggtgtt attgaaggct ctggaacggg
     4681 cgcagccttc gccaagacaa ctttattacc agggttcgaa aaccccgtag cgtgcgggcc
     4741 gcaatcttcc tgaacggctc tcctgaatcg tccgatctgc tcgggcgcgt cgccggatgc
     4801 gcagacctca ttgtcgccgc tgacggtggg gcccgttacg cgctcgaggc cggggttgtg
     4861 cctcatctcg tcgtcgggga tatggactcg ttgggagaag acctggcgct agaagtagag
     4921 aggctcgggg ccgccctgga gcgccatccg gtgaggaaag acaagatgga cggccagctc
     4981 gccgtactgg ccgccaggga gcggggtgcg accgctgccg acctactctg cgccgggggc
     5041 gggaggttag acgccctctt cgcggtccca cacatcctgc tcgccgccga gcgcatcggc
     5101 ctgcgttcca cggtcgttgc cgaccgggta aggatgttcg tggtcgaagc cggttacagg
     5161 acggtagaag gagtgccgcg ggaaattatc tcgatcttcc ccctctcggg tcctgccgcc
     5221 ggcgtcaccc tggaagggat ggaatatccc ctgcagaacg ccagcctgga gcccggagac
     5281 accctcgggt tccataacga gttgatcggc agtgaagcca cggtgagcgt gggtaaaggc
     5341 gcagtcctgg tggtacacga gacagaaaat cccgtgacag ggcatacgga ctcccgcgcg
     5401 ttaacgtcct aagataacgg tcgtccatgc ccggcgcttt tcgacccgac acggtacttg
     5461 acagggtaga gcaccgcccc taccctgtcc cttccggtcc gtgggcgctc gggatgagct
     5521 ggcgagacct gctcttcatg cactggcccg tcggggtaga agatctcagg cccctcgtgc
     5581 cgccggccct tggcatcgac actttcgaag gcagcgcctg gctcggcgtc gtccccttcg
     5641 atatgaccgg cgtaagaccc cacttcctac ccgccgtccc cgggctctcg cacttccccg
     5701 agataaacct ccgaacctac gtaacggcgg aaggcaggcc aggcatctgg ttcttctccc
     5761 tggacgcccg cagccgcgta gccgtccgcc tcgcccgcgc cacatttcat cttccctact
     5821 tcgacgccga gatgtcctgc cgcgcctcag gcgacgaggt ctactacagg agtgtgagga
     5881 cgcacagggg ggcgccgccc gccgagttcg cggcccgcta cagtcctgta ggggaaacct
     5941 tcgagagcga gcgggggtcc atcgaggact ttctgaccga gcgctactgc ttgtacagcg
     6001 tggacgggag gggcaacgtc cgccgcgggg aagtccacca ccatctctgg cccctccaac
     6061 ccgccgaggc cgaggtcggc acgctcgcga tgacccagca gataggcctg aaactgcccc
     6121 agaccgcccc gatccttcac ttctccaaac gcctggacgt cctcgcgtgg ccaccgcgcc
     6181 gggtagcttc ctaagccagg tggttcgcat ttttggcggg ctcgaaaccc gcccctacag
     6241 catgcggtct tcgcggtccg tatacttggt gcatggtggg tggaaccgac atctttgctg
     6301 tgggtgtgct ttgaaagcgg atctcgtctc cggactcttg cccgtcaggg aagacggccg
     6361 catgctcctg ctccagcgcc caacgggcac ctgggagccg cccgccggac gtcttcagcc
     6421 gggcgagagc ttcgaagaag gggccgtccg cgagctctac gaagagaccg gtatgctggt
     6481 ctctccccaa cgcatcatcg ctacctgggt cggcgaggcg ccgagcggta gacgactggc
     6541 ttccgtcacc tacgcgggga agagtgatgt agcaggggta cgcctctctg aggagcatct
     6601 cgaccacagg tgggtaaccc tcggggagtg gatgtccttg ccgagctggt ggagccacga
     6661 gaacatccgg cgtattgcac ggcccgtagg ggcggttcgc gaaccgcccg tgccacgcaa
     6721 accgccccca ccgcccctac gcggggacac cggcgtcgtc cctgcaaacc tcggagccgg
     6781 ggctgtactc gtggatcttg gaggcatcga gccgcgggcg ctcctgctca ggcgccgcaa
     6841 accgcccgtt gggctctggg agaaccctgg ggggatgctc gaagagggcg aaaatttcga
     6901 agtctgcgcc agccgcgagc tctacgaaga gaccggcgtg ctggcccgga tcgaggaccc
     6961 gtggtgggcc agggtcgagc cctggcgcaa tcctgaagat ccggagctct acgccggcgt
     7021 gggctttctg gcgaggcata agggaggaga gatagaccct gaatcagcgg cccacgacgc
     7081 ctctcagtgg gtcacggaaa ccgggtggcg ttcgctgcgt acctggtaca cgaagagaga
     7141 atcagacgtg ctctggagtg caatcaggga gatgcaacga taaacgaacg ggagaacaga
     7201 accgaaatgg gtcaggagca tggcggttcg agaggggctg gcctgatgcg gctcgtggga
     7261 atagtcctgc tcgtggccgc tgtgctgttc tgggttgcgg ccccgaccgt actgctgacc
     7321 ccgctatcca cggcgcagaa ggcctggacg ggttcggcct tcctgatcct gggagaggta
     7381 gcgttctggg tcgccgccgt ggtgctcggc cgggaggtat tccggcgcta caggaggtat
     7441 ctcgatcccc gcggctggtt gggcgagaag cgtcattgaa cgagttcgag gttttacgca
     7501 ggatctccgc cctcctgcct cccgcgcccc ccgaggtgct cgtgcccctc ggggatgact
     7561 gcgccgttct ggagatcggg ggcagtacgt gggtagccgc ctccgatatg ctcgtctcgg
     7621 gccaccactt caaagactgg gccacgcctg aggacgtcgg ctacaaggcc gtcgcggtca
     7681 acgcctcgga cgtggcctcc atggggggaa ccccgaggtt cgtcctggcc tcgggaggag
     7741 cgcccggccc ggagaccgcg ctccgctgct ctcggggtat cctggaagcc tgcgaagagt
     7801 tcggcatcta tccgctcggg ggtgatacga cccgcgcgga tgccctcacg gtggacgttg
     7861 ccatcctcgg agagctcgcc acgcggccgg tactgcgttc gggagcgagc cccggcgacc
     7921 tccttgcggt gacgggcgag ctcggcgcct cggcggccgg actactcgcc ctcgaacgag
     7981 gcatgacagg cccggagcgt ctcattcgca ggtgcctgcg gcccgaaccg aggctaagcg
     8041 ccgggcgcgt cgccgccagg ctcggcgcca ccgccatgat agacctctcc gatgggctgg
     8101 cttcggacgt acgccgcctg tgcgagagga gcggggcggg gtgcatggtg gacctggatc
     8161 tcctgcccat caaagacgat accagagagc tcgcacgatc cctcgaacgc gaccccgaga
     8221 tcctcgctgc aacaggaggc gaagattacg agctactggt ctgtgttccc gagcctgtcc
     8281 tggacgcact ggcgagtgac ctcgaagtcc ccctcacact gatcgggcag atcacccggt
     8341 ccgatgtcgt tttcacgcgc gagggcgagc cggtcgaagg cttatccggc tggaaccact
     8401 ttacctgaga cctgttacct gtcgcctgac acctctgcct caagtgcccg ctcgacgcgc
     8461 cccaactcct ccctgatccg cccgagcagt tcgggggccc gcgagagatc taccgacgca
     8521 ccgagttcct ccaactgcgc acagagaccg gacattcccc ttgcgcccat gctgcccgat
     8581 ccgcctttga gcgcgtgcgc cgcccgttct acggcaggcg cgtcgccctt ctgcaaagcc
     8641 tcttctaccg cctcgagtcg cgagcgcgaa tccttcagaa acatgccggt gagctcggcc
     8701 acaatgtccg ggtcgccttc accctgcagc ctgcgcagcc gcgcgagtac ctccctgtcg
     8761 acagattcct ccatgtgctt cgtgccaagt tcagcgggca ccgatctctc gagttcggcg
     8821 tccacgtcct caggctccat cgatacctcc agcgctaaaa cccatcactt acctttagtg
     8881 tacgtcgtcg gcgaccgcac ggatgacccg caggccgaaa cgagagatag tgtgccccgg
     8941 cacttactct atggctctgc gaatcgcaca ttcgctagag ggcgaaacca aaaagctgaa
     9001 atgctgacag gcgacagccg caggctggag cgtgctgaaa tgctaactgg agaagcaagg
     9061 cgctctaagc acgcggcaga tacacggtga aggtcgaccc ttctccaggg gtactccgga
     9121 cggtaacccg gccgccgtgg
//
LOCUS       DATMVF010000104         7250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_653264, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000104 DATMVF010000000
VERSION     DATMVF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653264"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VN178_04715"
     CDS             complement(<1..443)
                     /locus_tag="VN178_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80304.1"
                     /translation="MSEARVAGAQGDARFRRYLGRPETPALAFLVLLVAAFSVLSDKF
                     LSVPNMESILTSVAVLGVIALAVNQVVLCGEIDISTGSMLGLCAVAGGAAAQSTGGFL
                     LPLLAAVAVGALCGAINGTLVTLGRIPSIIVTLGMLYALRGVILLV"
     gene            complement(436..1977)
                     /locus_tag="VN178_04720"
     CDS             complement(436..1977)
                     /locus_tag="VN178_04720"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWS80305.1"
                     /translation="MTEPEETRAEAPPVFRLDGVTKRFGGVVAVEDVAFDLRPGEVHA
                     LVGENGAGKSTLMKIVDGLYGPDEGMLEVGGEAASFSSPRDAEASGIAMIPQELDLFP
                     ELTVAENLFVGRKRPRTGWGGLDWETMRGEARKRLDELGVGLDATSAVKRLSAAEQQI
                     VAIARALVGEARAVIMDEPTSSLTERETRQLFGIIEDLTAEGVGVVYISHRLEEIFEI
                     SDRITVLRDGHHIKTAPASELDAEELVRLMVGRPLHELFTRSQSSPGETVLEVRGLTR
                     EGEFEDVDLALRRGEILGLAGLVGAGRTELAQSIFGIRHPDSGEIFVDGEEVRIHAPQ
                     TAMERGIFYVPEERRSQGLILPFSIKNNITLSILDRISRLGFVSRSERGIADRFMREL
                     SIRGAEVSAPVNRLSGGNQQKVVVAKSLAREPNVLLLDEPTRGVDVGAKSEIYRLMDD
                     LAKEGKAILLISSELEEVLSMADRVLVMREGRISGEFSRGEATQENVMTAATGTAGQE
                     VSEDE"
     gene            complement(1974..3470)
                     /locus_tag="VN178_04725"
     CDS             complement(1974..3470)
                     /locus_tag="VN178_04725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HWS80306.1"
                     /translation="MSAGYLAVDLGAESGRVVLGRFDGGRVSLEEFHRFPNTPVRLPD
                     GLHWDVLRILSEIKDGLAKAVREEEIEGIGIDSWGVDFGLLDGEGALVSNPYHHRDAR
                     TEGMMDEAFGLVGKEEIYRTTGIQFLPINTLYQLLAMRGSPLLEAAETMLLIPDLMNY
                     WLTGEKACEYTNATTTQLLDLQDGGWSTDLLKGLDLPSRILAPIVQPATELGPLLPGV
                     AEEVGAGPPVFAVASHDTASAVVAVPAEGEDFAYISSGTWSLVGVELPSPVTTEEGLR
                     ANFTNEGGFGGKTRLLKNVMGLWLLQECRRQWAREGYEYTYEELARLAEDAPPAGPLV
                     DPDHPAFLAPGDMASRIRSFCEETGQRPPQEPAAVTRCVFESLALKYRYALEQAENLA
                     GRAIGTINVVGGGSQNSLLCQLTADATRRPVLAGPVEATALGNLMVQAYARGHLASLE
                     EMREAVRRSVKVQEYEPQGAEDERQEAYEKLRDLVGAAPRLDQEREGT"
     gene            complement(3467..6187)
                     /locus_tag="VN178_04730"
     CDS             complement(3467..6187)
                     /locus_tag="VN178_04730"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HWS80307.1"
                     /translation="MAPSELRPVGLECEYRTDPLGIDERSPRLSWELQSESRGQVQSA
                     YRVLVAGNGEDLEAEKNLLWDSGRIESGRTIGVEYEGEALRSGSHYVWKVRVWDGIDD
                     PSPYSPPAVFETGLLERSDWEGTWISAGKGPAGDMEPPSGDEYDDLANGLAPSPYLRR
                     EFRLDKPVSRARMYATARGVYELYINGNRVGSDVLAPGWTDYDRRIQYQTYDVTPLLA
                     DGPNALGAVLGDGWFAGFFGFDPKHRGALYGTRPQLLAQLDVEYQDGTTESLATDGSW
                     SCSTGPILFSDLLMGESYDARREMPGWAEPGFDDSEWYGVEAEEIRNTNLVAQPDEGI
                     RVTEELEAKTVTEPESGRYVFDLGRNMVGWVRLKVEGQAGTRVTLRHAETLNPEGTIY
                     TENLRSARQTDTYILKGGGEEVYEPRFTFHGFRYVEVTGYPGEPPLDAVTGCVVHSAT
                     PPSGSFECSSSLVNELQENIVWGQRGNFLSVPTDCPQRDERLGWMGDAQVFVRTASFN
                     MDVAAFFEKWMADVEDAQSPEGAFPDVAPLLRGSGLIDLRWGAPAWGDAGVIVPWTIY
                     RTYGDTRIVERHYDAMARWMEYLHEANPGLIRKNRMGNNYGDWLSPRGDHTPKHLLAT
                     AYWAYDAKLMAEMAVATGRNEDAKKYGDLNERIKAAFQETYVSSDGRIEGDTQTCYLL
                     ALHMDLLSEELRSAAAEHLVRTIEREDWHLSTGFVGVGYLCPVLTEAGYTDVAYRLLE
                     KETYPSWGYTIRNGATTIWERWDGWTEENGFQSPNMNSFNHYSLGSVGEWLYRYVAGI
                     GLGTPGYGQIVIRPRPGGSLTHARAEYDSVRGRISSSWKIEDGRFVLEALIPPNTTAT
                     VHVPSTDDVTEGGRPVHDAYGVEFVRAGEGETVLAVGSGRYEFEGGMARG"
     gene            complement(6191..>7250)
                     /locus_tag="VN178_04735"
     CDS             complement(6191..>7250)
                     /locus_tag="VN178_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80308.1"
                     /translation="LIVFSLNLPMLLIGTFIVGVAVGADVPTSLALIGEFSPSKARGR
                     LLGLSQAAWSLGPVVVYVLAFALSSYGVLGNRIVFAHLFVVAIVTWALRRGMVESAIW
                     TAASGAGEAESTRAPEEQPEEAGVPADPLAASRLRNLFLGPTLAAMVFTATVYLFWNL
                     AAGTFGVFQPYILKTLGTQSQAVSVALSCAGFVLTLLASVLIFMPFSDRSDRARRTMW
                     GIGAVLNVASVGQLLILPFNTVTAVALVVLFGIGAGIAGEAFYKTWSQELFPTMLRTT
                     AQGLTFSVARLLLGIWSFFVPVLASLSIRPVALILMIFLTISGVVGYFFMPNTAGKSL
                     EEIEAERSGRPVRERATV"
BASE COUNT         1246 a         2414 c         2287 g         1303 t
ORIGIN      
        1 accagtaaga tcacgcccct gagcgcgtac agcatcccga gcgtcacgat gatggaaggg
       61 atacgcccca gcgtgacgag agtaccgttg atcgccccgc aaagggcccc gacggcgacg
      121 gcagccagca gcggcagaag gaacccgccg gtactctgtg ccgcggcgcc cccggccacc
      181 gcgcagagac ccagcatcga gcccgtcgag atgtcgatct ccccgcaaag gacgacctgg
      241 tttaccgcga gggctatgac cccgagcacg gccacggagg tcaggatgga ctccatgttg
      301 ggcacgctga ggaacttgtc gctgagcacc gagaaggccg ccaccagcaa gaccagaaaa
      361 gcgagcgccg gagtctccgg acgacccaga taccggcgga accgcgcatc gccctgcgcg
      421 cctgcgaccc gcgcctcact catcctcgga aacctcctgc ccggcagtgc cggtagccgc
      481 ggtcatcacg ttctcctggg tcgcctcacc gcgcgagaac tcacccgaga tacggccctc
      541 gcgcatcacc agcacgcggt cggccatcga gagtacctct tccagctcgg aggagatcag
      601 caagatcgcc ttgccctcct tcgccaggtc gtccatgagg cggtagatct ccgacttcgc
      661 gccgacgtcg accccccgcg tgggctcatc aagcagcaag acgttcggct ctcgggcgag
      721 ggacttggcg acgacgacct tctgctggtt gccccccgag agccggttca cgggcgcgga
      781 gacctcagcc ccccttatgg acaactctct catgaacctg tccgcgatgc cgcgttctga
      841 gcgggatacg aacccgagcc gggagatgcg gtcgaggatg ctcagggtga tgttgttctt
      901 gatggagaaa ggcaggatga ggccctggga gcggcgctcc tcgggtacgt agaatatccc
      961 cctctccatc gcggtctgcg gggcgtggat cctgacctcc tccccatcga cgaagatttc
     1021 accagaatca gggtggcgta tcccgaagat ggactgggcc agctcggtac gccccgcccc
     1081 gacgagcccg gcgagtccca gaatctcccc cctgcgcaat gcgaggtcca cgtcttcgaa
     1141 ctccccctcc cgggtgaggc ccctgacctc gagcaccgtc tcgccgggac tgctctggct
     1201 gcgggtgaag agctcgtgga ggggccggcc gaccatcagg cggacgagct cttcggcatc
     1261 gagttcggag gctggcgcgg tcttgatgtg gtggccatcc ctgagcacgg tgatacggtc
     1321 ggagatctcg aagatctcct ccagcctgtg ggagatgtac actacgccaa cgccctcggc
     1381 ggtgaggtct tcgatgatac cgaagagctg gcgggtctct cgctcggtca gggaagaggt
     1441 cggctcgtcc atgatcacgg cccgcgcctc gcccacgagc gcccgcgcga tggccacgat
     1501 ctgctgctcg gccgccgaga gccgctttac ggctgatgtg gcatcgaggc cgactccgag
     1561 ttcgtcgagc ctcttgcgcg cctcgccccg catggtctcc cagtccaggc cgccccagcc
     1621 ggtacggggc cgcttgcggc ccacgaacag gttctcggcc acggtgagct cggggaacag
     1681 gtcgagctcc tgcgggatca tggcgatgcc tgacgcctcc gcgtcgcgcg gcgaggagaa
     1741 agaggcggcc tcaccaccca cctcgagcat cccctcatcg ggaccgtaga gaccgtccac
     1801 gatcttcatc agggtgctct tgccggcgcc gttctccccg accagcgcgt gtacctcacc
     1861 cggccgcaaa tcgaaggcga catcttccac cgcgaccacg cccccgaacc gcttggtaac
     1921 gccgtcgagc ctgaagacgg gcggcgcctc ggctcgggtc tcctccggct ctgtcaagtg
     1981 ccctctcttt cttgatccag ccgcggcgcg gccccgacaa ggtctcgcag cttttcgtaa
     2041 gcctcttgcc gctcgtcctc tgcgccctgc ggctcatact cttgcacctt aaccgagcgg
     2101 cgtacggcct ctctcatctc ctccagcgaa gccaggtgtc ctctggcgta agcctggacc
     2161 atgaggttcc ccagcgccgt agcctcgacg ggcccggcca gcaccggtcg tcgggtcgcg
     2221 tctgcggtaa gctggcagag gagcgagttc tgggatcctc caccgaccac gttgatcgtc
     2281 ccgatagccc ggccggcaag gttctcggct tgctctaggg cgtacctgta cttgagcgcc
     2341 aggctctcga agacacaccg cgtaaccgca gcgggctctt gtggcggtct ctgtccagtc
     2401 tcctcgcaga agctccgtat gcgcgaggcc atgtctccgg gagccaggaa cgcgggatgg
     2461 tcgggatcga cgagcggacc ggctggcggc gcgtcttcgg cgagacgggc caactcctcg
     2521 tatgtgtact cgtagccttc cctcgcccat tgcctgcggc actcctgcag caaccagagg
     2581 cccatgacgt tcttcaagag gcgcgtcttg cccccgaacc cgccctcgtt ggtgaagttg
     2641 gccctaagtc cttcttccgt tgtgacggga ctggggagtt ccaccccgac gagagaccag
     2701 gtgccgctgg agatgtaggc gaagtcctcg ccttcggcgg gcaccgctac cacggccgag
     2761 gccgtgtcgt gtgaggcgac cgcgaatacg ggcggcccgg cgccgacttc ttccgccact
     2821 ccgggaagga ggggacctag ttcggtggcc ggttggacga tgggcgcgag gatgcgggag
     2881 gggagatcca gtcctttcaa gagatccgtg gaccatccgc cgtcttggag atccagtaac
     2941 tgcgtggtcg tggcgttggt gtattcgcag gccttctcgc cggtgagcca gtagttcatg
     3001 aggtcgggga tcaggagcat cgtctcggcg gcttcgagaa gaggagatcc ccgcatggcg
     3061 aggagctggt agagggtatt tatgggcagg aactgtatgc ccgtggtccg gtaaatctcc
     3121 tccttcccca cgagcccgaa ggcctcatcc atcatgccct ccgtgcgcgc gtcacggtgg
     3181 tggtaggggt tggagacgag cgccccttcc ccgtccagaa gcccgaaatc aacgccccag
     3241 gaatctatcc cgataccttc gatctcctct tcccgcacgg ctttcgccag tccgtctttg
     3301 atctcggata gtatccgcag aacgtcccag tgcaacccgt cgggcaggcg aacgggcgtg
     3361 ttcgggaagc ggtggaactc ctctagcgac acccgcccgc catcgaagcg tccgagcacg
     3421 acgcgcccgc tctcggcccc caggtctacc gcgagatacc cggcgctcat cctcgcgcca
     3481 tcccgccctc gaactcataa cgtccggatc caacggcgag aacggtctct ccctcgccgg
     3541 cgcgcacgaa ctcgacgccg taggcatcgt gcacgggcct gccgccctct gttacatcgt
     3601 ccgtggaagg cacgtgtacc gtggccgtgg tgttgggggg gatgagcgct tccaggacga
     3661 accggccgtc ttcgatcttc caggaactcg agatcctacc cctaaccgaa tcgtactcgg
     3721 ccctcgcgtg cgtcagagag cctccgggac gcgggcggat gacgatctgg ccgtagcccg
     3781 gtgtccctaa gcctatccca gccacgtagc gatacagcca ctcgccgacc gagcccagag
     3841 agtagtggtt gaaggagttc atgttcggcg actggaagcc gttctcttcg gtccagccgt
     3901 cccagcgctc ccagatcgtg gtggccccgt tccttatggt gtatccccac gaagggtagg
     3961 tctccttctc cagcagacgg taggcgacgt ccgtgtaccc ggcctcggtc aggaccgggc
     4021 agaggtagcc gacgcctacg aaccccgtcg agaggtgcca gtcttccctc tctatggtcc
     4081 tcaccaggtg ctctgcggcg gcggatctca gctcttctga tagaaggtcc atgtgcaggg
     4141 cgaggaggta gcacgtctgg gtgtcgccct cgatccggcc gtcggaggag acgtaggttt
     4201 cctggaaggc ggctttgatg cgctcgttga ggtccccgta cttctttgcg tcctcgttgc
     4261 ggcctgtggc cacggccatc tcggccatga gcttcgcgtc gtaagcccag taggccgtgg
     4321 cgaggagatg tttgggtgta tgatcccctc tcggtgagag ccagtcgccg tagttgtttc
     4381 ccatcctgtt cttgcgaatg aggccggggt tggcctcgtg cagatactcc atccagcggg
     4441 ccatggcatc gtagtgccgc tcgacgatgc gcgtgtcgcc atacgtgcgg tagatcgtcc
     4501 acggcacgat aacgccggca tcgccccagg ccggggcgcc ccacctcagg tcgatgagtc
     4561 ccgaacctct gagcaggggg gccacatcgg ggaaggcgcc ttcgggggac tgggcgtcct
     4621 cgacgtcggc catccacttc tcgaagaagg cggcgacgtc catgttgaaa gaagcggtgc
     4681 ggacgaagac ctgggcgtcc cccatccagc cgaggcgctc gtcgcgctgc gggcagtcgg
     4741 tcggcacgct caggaagttg ccgcgctggc cccagacgat gttctcctgg agctcgttga
     4801 ccagagaact ggagcactcg aaggaccctg aaggaggcgt agcggagtgg acgacgcatc
     4861 cggttacggc gtcgagcggc ggctcgccag gatagccagt tacctcgacg taccggaacc
     4921 cgtggaacgt gaaacgcggc tcgtagacct cctcgcctcc gcccttcaag atgtaggtgt
     4981 cggtctggcg ggccgacctc aggttttcgg tgtagatcgt accttctggg ttcaaggtct
     5041 cggcgtggcg cagggtcact ctggttcctg cttggccttc cactttgaga cgcacccacc
     5101 ccaccatgtt cctgccaagg tcgaagacgt aacggccgct ctcaggctcg gtgacggtct
     5161 tcgcttccaa ctcttcggtg accctgatgc cttcgtcggg ttgggctaca agattggtgt
     5221 tccttatctc ctctgcttcc accccgtacc attctgaatc gtcgaaaccg ggctcggccc
     5281 agccgggcat ctcccttcgg gcgtcgtagg actcgcccat gaggaggtct gagaagagaa
     5341 tagggcccgt cgagcaactc cacgagccgt cggtcgccag gctctctgtc gtgccgtcct
     5401 ggtactcgac atcgagctgc gctagaagct gtgggcgcgt tccgtagagg gcgcccctgt
     5461 gtttgggatc gaaaccgaag aatcctgcga accagccgtc gccgaggacg gcgccgaggg
     5521 cgtttggtcc atcagccaat agcggcgtga cgtcgtaagt ttggtactgg atgcgcctgt
     5581 cgtagtccgt ccagcctggg gcgagcacgt cgcttcctac cctgttcccg tttatgtaca
     5641 gttcgtagac gccgcgggcg gtcgcgtaca tccttgccct gctgaccggt ttgtccagtc
     5701 gaaactccct gcgcaagtac gggctcgggg cgagtccgtt ggccaggtca tcgtactcgt
     5761 ctcccgacgg cggctccata tctccggccg gacctttgcc ggcggagatc caggttccct
     5821 cccaatccga cctctccagc aagcccgtct cgaagacggc cggagggctg tagggcgagg
     5881 gatcatcaat cccgtcccag acacgcacct tccacacgta atgcgagcca gaccgcagtg
     5941 cctcgccctc gtactcgacg ccgatggtgc ggcccgactc gatcctgccg ctgtcccaca
     6001 gcaggttctt ttccgcttcg aggtcttccc cattgccggc gaccaggaca cggtatgccg
     6061 actgtacctg tcctcggctc tccgactgga gctcccagct gagcctgggc gatctctcgt
     6121 cgataccgag ggggtcggtg cggtactcgc actcgagtcc tacgggcctg agttctgagg
     6181 gagccatttg tcagaccgta gcccgctcgc gcaccggtcg gccgctacgc tccgcttcga
     6241 tctcctccag cgacttgcct gcggtgttcg gcatgaagaa gtagccgacc acgccgctga
     6301 tcgtgaggaa gatcatgagt atcagggcta caggtctgat gctcaacgac gcaagcacag
     6361 gcacgaagaa gctccagata ccgaggagca accgggcgac gctgaacgtg agcccctgcg
     6421 ccgtcgtcct gagcatggtc gggaacagct cctgcgacca cgtcttgtag aaggcctctc
     6481 ccgcgatccc ggccccgatg ccgaagagga cgaccagagc gacggccgtc accgtattga
     6541 aggggaggat gagcagctgg ccgaccgagg caacgttcaa tacggctccg atgccccaca
     6601 tcgtgcggcg ggctcggtcg ctgcggtcgc tgaaaggcat gaagatcagg acggaggcca
     6661 acagcgtgag cacgaagccc gcgcacgaga gggcgacgct caccgcctgg ctctgcgtcc
     6721 ccagggtctt gaggatgtag ggctggaaaa cgccgaaagt acccgcggcc aggttccaga
     6781 acaggtatac ggtcgccgtg aagaccatcg ccgccagcgt aggaccgaga aacaggttgc
     6841 gcaagcggct cgcggcgagc gggtcggcag gcacgcccgc ctcctcgggc tgctcttcag
     6901 gggctctcgt gctctccgcc tcgccggcgc ccgaggctgc ggtccagatg gcggactcca
     6961 ccatgccacg cctgagggcc cacgtgacta tggcgaccac gaacaggtgg gcgaagacta
     7021 tccggttgcc gagcacgccg tacgaggaga gcgcgaacgc cagcacgtag acgaccacag
     7081 ggcccagact ccaggccgcc tggctgagac ccaagagcct gccgcgggcc ttgcttggcg
     7141 agaactcgcc gatcagggcg agagaggtcg gcacgtcggc accgaccgcg acgcccacga
     7201 tgaaggttcc gatgaggagc atcggtaggt tgagcgagaa gacgataagc
//
LOCUS       DATMVF010000105        56222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_384357, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000105 DATMVF010000000
VERSION     DATMVF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 56222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56222
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384357"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VN178_04740"
     CDS             complement(<1..730)
                     /locus_tag="VN178_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWS80309.1"
                     /translation="MADRRPEAYITGIGAVTPIGVGRKKLWSSLLANESGAGPITLFD
                     PEGFEVRIAAECTDFDPESFMDRKVAARTDRFGQMGLASAKLALEDADAWDVLNNNPE
                     RVGLILGSGIGGVTSMETTQATIDSKGPSRVNPFAVTKIMPNSAAAHVGIQLGVQGPS
                     ITGALACTCGTDMMGLGYDLIRRGDADVVLCGASEAGLAPVIVAGFIAMRAMSRRNDD
                     PEGACRPYDVDHSGFLIGEASAVMV"
     gene            843..1151
                     /locus_tag="VN178_04745"
     CDS             843..1151
                     /locus_tag="VN178_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80310.1"
                     /translation="MIFYIAMVLFVANFALGILVQFRIVDTKPFRWLHHALFFAVFAS
                     AAVAVGFGFWQGAPYRWILLPVLVLFAFLPRVRAGTPGHAALASGAMILYVTGFVWML
                     "
     gene            1162..1965
                     /locus_tag="VN178_04750"
     CDS             1162..1965
                     /locus_tag="VN178_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWS80311.1"
                     /translation="MDFLEVLKGRKTTNGPFLPDPVKPEHQRLLVEVAAMAPSHFNSQ
                     PWRFVLVDDRETIDEVARISGESMTRLLEEGTFWKRYRPYFRFSEAEMEEKRDGIHID
                     QMPSVLKPFSRQIFSNTGQAVMNRFGVPRTLGEDNRKLVAGSPLLLAVLLDKTEYRPG
                     ELSGFYSIFGMGAAVENIWLTTTELGMGIQFVSTPMEIPENWERLKRLLKVPDDLELM
                     AVYRLGYVPDGQRRPTIDWSSRQRKRISQYVFRDTCEKPEPDFTPQEER"
     gene            1962..3008
                     /locus_tag="VN178_04755"
     CDS             1962..3008
                     /locus_tag="VN178_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HWS80312.1"
                     /translation="MTVPRILSVASALPPHRVGQEEAKTFARGMFSHGRGNIERLLPI
                     FDNVNIENRYFCVPAEWFERDHSFPEKNALYVEHALDLSEKAARRALDRVGARPGEVC
                     AIYFVSTTGVSTPSIDSKLLFRLGLSEHTRRVPIWGLGCSAGAAGLARAAEHARLYPD
                     QLVLLVAVELCGLTFQRGDRSKSNLISTSLFADGAAAVLIGGATGPELLGGYSTTWPG
                     TEDVMGWDLVETGLKVQLSKSIPNIVREKFRDNLEEACNSLGLDFDELEHFVLHPGGS
                     KVLDAFEEALGLQAGELATSRDVLRDCGNLSSVTVLFILERFLESGGLAKGDLGVLSA
                     MGPGFSAEHVFFRC"
     gene            3096..3602
                     /locus_tag="VN178_04760"
     CDS             3096..3602
                     /locus_tag="VN178_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWS80313.1"
                     /translation="MLLVMGVMLVGVQRILELALSRRNERRLRARGAVERGSGHYPVI
                     VAIHTLWLVSTLVEGLLRGPEPPAWWPVPLVAFLLVQPLRFWAILSLGENWNTRVLVV
                     PGGKLVRRGPYRYFPHPNYVVVAVEVLTFPLIFGAWITAIVFSLLNAALLYVRIKTEN
                     RALGELAG"
     gene            3839..5107
                     /gene="serS"
                     /locus_tag="VN178_04765"
     CDS             3839..5107
                     /gene="serS"
                     /locus_tag="VN178_04765"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWS80314.1"
                     /translation="MLDLKFIRENAKAVEENTKNRGVEADVGLVVELADRRSELIQES
                     NELKHRQNEMARSIGRERDEEIRTKLIEESRAMKDRLPSKERELHEVEERLRDEQSKI
                     PNMTHPDSPIGKDDTENVEIRRWGEIPDFPFEPKDHVELGESLGIIDFDAGAKTTGTK
                     FYFLRGDAVLLELGLIRYSMDIIMEHGYQPTITPDLARDRMLVGTGFVPRGPETQIYS
                     VEDTDLSMIATAEIPLAGQHADEIIDEGDLPLRYAGLSHCFRTEAGSHGRASRGLYRV
                     HQFTKVEMFAFTTPEQSEDVHEEMVGIEERIFQGLGLPYRVVDICTGDLGGAAYRKYD
                     LEAWMPGRNDFGEVTSTSNTTDYQARRLAIRYRKEGGRPQLLHTLNGTAVAMSRALVS
                     LLEIYQQQDGSVILPEVLVHYVGKARLTPA"
     gene            5328..6758
                     /gene="proS"
                     /locus_tag="VN178_04770"
     CDS             5328..6758
                     /gene="proS"
                     /locus_tag="VN178_04770"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWS80315.1"
                     /translation="MTEAVEKLVKKSEDPSRWYLQLVRMAKLADYGPVRGTFAIRPYG
                     FEIWERVQRDLDDRFKATGHRNAYFPLLIPESYLKKEAEHLEGFDPELAWVTIGGREE
                     LEERLAIRPTSESIICDFYRNWIQSYRDLPILINQWCNVLRWEKVTRPFLRSAEFLWQ
                     EGHTVHATAEGAREESLRMLGVYRDCFYEAMAIPVLTGAKSPSERFAGAVETYTCEGL
                     MGDGRALQAATSHDLGQNFARAFDITFLDENQDRVYPYQTSWGFSTRTIGGLILVHGD
                     DRGLRLPPRIAPTQAVIVPIWRGKNKGEVRREAEALHTQLKDFGIRMEVDLDEEHSPG
                     WKFNEHELRGVPVRVELGPRDIEKEQAVLVRRDTGEKEFVGRKVLPERLKALLGEIQA
                     NMLRQATEFRDANTRRAESYEEFEEIIARDRGFVVAPWDGMKETEQKIKEDTRATIRL
                     LPFEREEGKDLVSGRPGRTAIFARAY"
     gene            7188..8075
                     /locus_tag="VN178_04775"
     CDS             7188..8075
                     /locus_tag="VN178_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80316.1"
                     /translation="MPVLILLAGDHIAVEAEVYSAYSDPKKPVIAVILSERQNVDEFK
                     AHFGLTGGQVDAVLAATRLENRILAQEYGESERVLAANKGLPKRELAKKIVASDYDEK
                     VAAAVAGTKKKIEAIVPEDQSADLEGWVDEQWNQEVQAASTEDYAATSESYMVSSLSK
                     GVRCKVFATQYIGYTEREVALPHRNLKLGRQPRVRIWRVSGGPSVGPRVKEVGPWNTY
                     DNYWVRNEDRTMYERVPRCKPQAQVAYYQNFNKGKDEFGRKVLNPAGVDLTPDVARSM
                     GLKKYQNAWVWVRFPWVHR"
     regulatory      8159..8283
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            8369..9493
                     /locus_tag="VN178_04780"
     CDS             8369..9493
                     /locus_tag="VN178_04780"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWS80317.1"
                     /translation="MTLEPRAGASQAPALRSHRIGSFQPELGGSLRDVALAYETWGEL
                     NEAADNAVLLVHALTGDSHAAGGPSEGYRRGGWWDPMVGPGLPLDTEKYFVVCSNVIG
                     GCSGSTGPASIEPGTRRPYGMRFPSVTIRDMVRVQRRLLDSLGVRRLALVAGGSIGGQ
                     QALEWAVEYPDFVEKAIPVAATGALGPQGIGMSEIGRRAIMADPDWQDGDYYGTGRTP
                     DRGLAIARMAGMMTYQSAAGQWERFGRRPAGRPALYPEFGGRFEMESYLHYQGRELTG
                     RFDANSYLYLSRAMDLYDVASGYESEEEAYSRIRAEVLFVGITSDWLFPAAEVRGAKE
                     KAKMAGAEAHYAEIDTLSGHDAFLKDWKELEEAIQPFLAK"
     gene            9546..10844
                     /locus_tag="VN178_04785"
     CDS             9546..10844
                     /locus_tag="VN178_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HWS80318.1"
                     /translation="MTDQKIQERHYGFDTLALHAGQEVDSATTARAVPIYQSTSYVFH
                     DTDHAADLFSLAEEGNIYTRIMNPTTDVFEKRVAALEKGVGALATASGQAAQGLTILN
                     IMGAGDELVSAAELYGGTYNQFHYTLPKVGIDVKFADSTDLDAIRAAITDRTKAVYAE
                     TVGNPALNTLDIEGAAAVAHDAGVPLIIDNTLPSPYLVNPLEHGADIVVHSATKFIGG
                     HGTSIGGVIIDGGAFPWDNGKFPGFTEPDPSYHGLEIYPTLGELSFILKARVQMLRDW
                     GPALSPFNSFLFLQGLETLPLRMERHSTNAQAVAEFLQGHPKVNWVNYPGLEDHPSHE
                     LAAKYHREGLFGAILGFGIEGGLEAGKRFINGLELHSLLANVGDAKSLVIHPASTTHS
                     QLSVDEQKSTGVTDDYVRLSVGLESIDDILYDLDTALNGA"
     gene            10849..12075
                     /locus_tag="VN178_04790"
     CDS             10849..12075
                     /locus_tag="VN178_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWS80319.1"
                     /translation="MARPLVIKFGGTSVGGGAQFVRAASIATEAARNNSVAVVVSAMG
                     GTTDTLLGYARMSSPWVRQTRKDETHEAPVAELRRMLAERHLRAAREAVAAEHLPGVE
                     GRVVDLLGELEVVLGTHSGNVKARRDEIAVYGERLSAEILAGAISSQGIPSGVVGADP
                     IATDTRFGEAEVDPAATRERTVRYVSPLLEGGLVAVVPGYVGRSPGGLPTTLGRGGSD
                     LSATVLGRALDSGEVWIMSDVDGVLDADPRLVPDASLMPRLSYREAGIFAGLGAKVLH
                     PRTMEPASEAGIEVFVRNTFAPEGSGTHVTGFDPGAGVRCVALRQNVAMEIPCTDGRE
                     GRAAMVVCIGSPDEEDLARGRRCLREAGIPVQHFGIAAAGLVFLVSADVGLEALRALH
                     SCLIPPAGDFAGEEVA"
     gene            12072..13136
                     /locus_tag="VN178_04795"
     CDS             12072..13136
                     /locus_tag="VN178_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80320.1"
                     /translation="MRRLEIVQLGLGHVGRAVAQIVLEERKRWLERHGIEMRYRAVSD
                     TSGALVGEELLPQAIRLKEEGGTLAELGVEPLEGVLGSEPEPGFTRVVVDVAVHGGTF
                     DLDVLGIRGGAFLVLSNKGPLSGSTAQYRQLMRMIPDRLWHEATVGAGMPIISTIGGL
                     QETGDEILEIQASPSGTLGFVMSAVEGGRSFSQAVREAVDLHYAEPDPRDDLSGLDVA
                     RKAIILARMMGREIEPHEIPYESLVPQGLEDVPLAEFMERLPEHDESFAARLLAVEEH
                     HMLRYLVRITKHGPVTVGLQDTPVESPFGPISGPENVFDFRTRRYSDVTLTVRGPGAG
                     PQRTASGVVWDLLDIAHKAL"
     gene            13187..14149
                     /locus_tag="VN178_04800"
     CDS             13187..14149
                     /locus_tag="VN178_04800"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWS80321.1"
                     /translation="MGDGYTLAVVGAGLVGDRLVAELRRRDFPLREVRILARSARTAV
                     LAGETFQVGVAEPEAFEGVDIAFFAGTEGEKGAAVQLADEAISRGAVVIDNGSDFRLD
                     PSVPLVVPEVNVGALEGHQGLIANANCSTTQMVVTLAPLAREFGLVKVVVSTYQAVSG
                     AGRNGVEALENGREDAFPRPIVGNAIPQIGGFGDDGYTTEEAKMREETRKILSLPDLP
                     VFATAVRIPVHTGHAESVYVETEREANLPAVLEALGTAPGVEVTDDFPTPIEVAGEDG
                     THVGRVRVEGRVIQYWCVADNLLKGAATNAVQIAESLVGVPARA"
     gene            14245..14520
                     /locus_tag="VN178_04805"
     CDS             14245..14520
                     /locus_tag="VN178_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80322.1"
                     /translation="MAQGEKRSFWDRLFGRNQPSAREERVVEYIIHRLGEGAHLGDVV
                     KEEYVRRNASPLEIEEICADPRLVEAARQHLRQDFDSGELDPGKRPR"
     gene            14596..14901
                     /locus_tag="VN178_04810"
     CDS             14596..14901
                     /locus_tag="VN178_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWS80323.1"
                     /translation="MPIYEYKCENGHVFDVMQKLSEDPLSSCIECGAPVRKVLHPVGI
                     SFKGSGFYSTDYSKKGPKEAAQKDTSKSANGDSGSSSEEKKGKEKPAESSTSSGKKD"
     gene            14906..15157
                     /locus_tag="VN178_04815"
     CDS             14906..15157
                     /locus_tag="VN178_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80324.1"
                     /translation="MARGADLPQSRDRSESILRKALRAVVLGVTLLFVVSALAVSCAL
                     VVYTAWDHDLSHGIFALLFAVLSAQIAINWFLWKDDEEQ"
     gene            15494..18970
                     /locus_tag="VN178_04820"
     CDS             15494..18970
                     /locus_tag="VN178_04820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWS80325.1"
                     /translation="MAVTSGDAFAHLHCHSEYSMLDGASRIGDLISFAKDQGMPGLAL
                     TDHGVMYGAVKFYKEARAAGIKPLVGCEVYVTADRNDRSRAPYYHLTLIARTAEGYRN
                     LMKLSTCGFLEGFYYKPRVDMEMLRKYGRGIVCLSGCLSAEVPARILEGRPDEARSLL
                     LEYAEIFDDVYLELQDHGIEQQRRVNEGLFKLHKETGIPLVAANDSHYTTRNDARMHD
                     VLLCIGTGKFHNDPNRMKFSGQEFYVKTAEEMARIFPDHPDALENTIRVVESVEDVGI
                     ELGKTRLPNFPKPEGYTADQYLREQCQRGLVRRYGGRAKSREVLGRLEFELQTIEKMG
                     FADYFLIVWDFVKYAKDRKIAVGPGRGSAAGSIVAYALEITDLDPLEYSLLFERFLNP
                     DRINMPDVDIDFSVSGRSEVMRYVTDKYGGHEHVAQIITFGTIGAKAAIRDSGRIYQY
                     PYSDTDKLAKFIPDKPVGTTLRDVLRPENGSYVAGDKHPGPAREIMKFVGQDEAAKQV
                     LDTAFEIEGFARHAGTHAAGVVISEEPLTDIVPLQRVARDEGAVMVQHPMSDVEALGL
                     LKVDFLGLRNLDVIEETLETIRRGGGEEPDIRSIPLDDEKTLRLFARGDTFGVFQFES
                     SGMQRMLQEVRPDRFDDLVALNALYRPGPMDYIPNFKRGKHDPESVEYLDERLKPILE
                     PTYGVAAYQEQLMEISKSLGGFTPGEADTLRKAIGKKNAAMLATLRDKFMAGCAASGV
                     STDGAGELWNWMEKAGGYSFNKSHSACYSFLAFQTAYLKAHYPEAYMAALMSSVMNTK
                     DRVPQYVAEARAMKIQVLPPDVNESGRRFTVVGETIRFGLSAVKNVGDSCVDSIIAAR
                     EDGPFQDIFDFCERVDPKTYNKRTLESLIKCGAFDNVGPSRAAMVAVHAKAVERSTRG
                     LKGAHEDQFSMFDVSELAPPRPEFPDVEEDQRQSLEWEKEALGLYVSDHPLRPVLHKL
                     RKHTDTTVSDLELYKDGAIVWVGGLATSVRTNTTRKGDMMAMLQLDDTRGFAEVMVFP
                     RVYAKFSACVREDAVLKVKGKVEMKEGIPRIVALEMEELHLEPGADPVYLDAGAFVGH
                     SQNVARVAFELLLRHPGESPIFLVSGEEMVEEKIGNITDSSDLHAELKRLLGPRCLSY
                     TSPAELPMERVS"
     gene            19215..20303
                     /locus_tag="VN178_04825"
     CDS             19215..20303
                     /locus_tag="VN178_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWS80326.1"
                     /translation="MADKIVFTRGVPPPEAFPTEELGACLDAAVRENPDVVLQYGQQP
                     GYAPLREELAGEYGVSPQEILIGNGSLQLQDLVSSYLVEPGAAVYTEQPSYDRALKTF
                     RRRGARIVGIPLEEDGISVERLEAALGREVPAFLYLVPDFQNPAGATLSLEKRQRVVE
                     LADRYGFWVIEDIPYRKLRYEGEDVPLLREIDPSHVITMSSFSKLLSPGMRVGFMISP
                     EDLIREVTALGEDTYLSPVLPTQAAVAEYLRRGLLGPNVERLKDLYTPRWKAMAGAVR
                     RELPGAQAFIPSGGFFVSVMLPEDANTENLVGRAGDIGLVLTPGAAFFADAEEGDVAD
                     GGRFVRLPFCAVTPDQIEEGVRRLTSLL"
     gene            complement(20310..20696)
                     /locus_tag="VN178_04830"
     CDS             complement(20310..20696)
                     /locus_tag="VN178_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS80327.1"
                     /translation="MDLHLDLIGIVARDMRASMEFYRRLGLDIPEGAEDEPHAEVTTP
                     SGLRVAWDTAELIQKIDPEWTEPSGGHRVALAFLCPNPASVDAKYEELAALGFGHKVP
                     WDAFWGQRYASVKDPDGNQVDLFAPL"
     gene            20891..22255
                     /locus_tag="VN178_04835"
     CDS             20891..22255
                     /locus_tag="VN178_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWS80328.1"
                     /translation="MDEQVTHAREHADLKRGIGPWLLFFFVLGDIVGAGIYALVGEVG
                     AVIGGAIWVAFLCAFILAIFTAASYAELVTKYPRAGGSATYVNNAFRNSFVSFMVAFA
                     VAAASLTSASVLSLAFSGDYLLQFINVPSVLAALVFVVVIGFINFYGISESVRLNLVF
                     TCVEITGLVLIVVIGFVALGAGTGEPARALEFKEGTSILPAILAGTVLSFYALIGFDD
                     SVNVAEEVRHPSRIYPRAIFGGLLAAGVIYLLVTFTASMVVPTAELAASSGPLLEVVE
                     RSPLAIPTKLFALIALIAVSNGALINLIMASRVIYGMGDQDVLPEVFSRVHPSRRTPW
                     VAILFATLVVFTLLATVGRNPTALDTLSTTTVVLLLLAFIMVNVSVLALRSDEVDHEH
                     FRTPTVFPILGIVVAATLLIYTAIDDWTVFGLAGLLLGIGLILYGVNVLVKRSLGESL
                     QSGE"
     gene            22471..23841
                     /locus_tag="VN178_04840"
     CDS             22471..23841
                     /locus_tag="VN178_04840"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS80329.1"
                     /translation="MNGLLKGALAAGGGAVGVELLRRALFSGARRRYEPWERAPYKDF
                     PNRVLIVGGGFAGYKAAQSLCELARDRDDVGVMLISRENYFTFWPMLASVIGGDVATQ
                     NVAQPLRRTLIRLGASFRRAPLDSVDLKRRCVVAGGKEFPYEHLVLSLGAEPAYFGIP
                     GVEEHCISIRGIGAGETIVDRVIERYEEVTLAGGEIPDSKLTFMVIGGGATGVEVASE
                     LHSLVRDLLVPDYPNINPHRVKIILVDANKEILKELDPALRRVARKKLADLQIEVINN
                     VKAKKVTADRVILGDGREIMVENVIWTAGSRASVKLDEIDLPHDERRGLTVDRYMRVV
                     GHDNVWGIGDCAATVDEDGSPVPPNAQAAVQEGDAVARNILAVIDGGEPRPFRYKSLG
                     QLVELGSEFAVNDVLGVKFSGQLAALFWRLIYLFKLESPESRARIAADWLLDIFYDPP
                     VTQIRR"
     gene            23978..24196
                     /locus_tag="VN178_04845"
     CDS             23978..24196
                     /locus_tag="VN178_04845"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HWS80330.1"
                     /translation="MRAVSCALLCNCRYALRADDDERLVAVALEHLRRYHPVAPVGEE
                     WVREIVTARAYEIEYAAVYPGNLGTEEV"
     gene            24419..25828
                     /locus_tag="VN178_04850"
     CDS             24419..25828
                     /locus_tag="VN178_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWS80331.1"
                     /translation="MRRTVVLSAAVAFAVLLVSGVWGSAAREAVGATAKPNIVFILAD
                     DMRKDDLKYMPKTLSVLRDKGMSFSNAFVSNALCCPSRATIMRGQYAHNTGVWSNKGS
                     RGGWKAYSSNGNEKDNMATRLDAAGYRTGLFGKYLNEYPGAKDKPPGWDRWFVHTDGS
                     SYYDYKINDDGKIVRYGSAPADYETDVIARHAAAFIGASAMARKPFFAFVAPRAPHSP
                     ATPATRDEHVHDGLKAPRLPSFDEKNVSDKPPWIRKLPGLSDARKAQIDNRAEKRAET
                     LQALDDLVAGIVGRLNDRHVLSNTYIFFTSDNGWHGGEHRIPKEKWRPYEEDVHMPLL
                     VRGPGVEAGRQAHKLVLDTDYLPTFTDLADTKMPDYVDGRSLEPVLEGNATAWRSAIL
                     LEASGRYSPAYRGIRTSDGRKYVEYAGGGKELYHLGRDAYELRDTYDPKNPPVSLAKR
                     LKTLVGCDAAGCREAEDGQ"
     gene            25888..27135
                     /gene="hisZ"
                     /locus_tag="VN178_04855"
     CDS             25888..27135
                     /gene="hisZ"
                     /locus_tag="VN178_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWS80332.1"
                     /translation="MDRKTPRKFATTPGTRDVLPPESTRLLDVQGRIRDRFALFGFRE
                     VLTPALEYAEVIEEHGLRDVSYKLFDQDNQMLLLRPEMTTPIARVISQRLAGSPPPHK
                     LSYVLPAYRRASVGRGQSAELYQAGVEVVGSASPTEDAATIALLVDVLQSLGLKAPSD
                     FAVVLGQSTFYAGYLRHVVPDAATAVTGALAAKDLVAVDDLAEELPAEAAAGVRGIPR
                     IVGPAADKSLIEEAGRFALGSEAREALENLREILRHLEAHGALGAVILDLGLSGRHEY
                     YTGAVYEAYAAGLGFTVANGGRYDNLLRCFGKELPATGFAIYLERLLSVLPEEGPQPL
                     LVLVGGDVGGIEAAARLRRLGVPVLHLSEDLPPEAAVEYARSVEAAWISYPTTGGVKL
                     AAADPAGEFASTSFESVARTVLP"
     gene            27132..27779
                     /gene="hisG"
                     /locus_tag="VN178_04860"
     CDS             27132..27779
                     /gene="hisG"
                     /locus_tag="VN178_04860"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWS80333.1"
                     /translation="MSVLRVAVPKGAIFVDALAALESAGLPAGVLRENGRRLVYRAEG
                     TEFIVSRPSDVPVFVEYGAADIGIVGKDVLDEQRPNVVELRDLGTGRCRMILAAPEGG
                     AEDVRRAIRHAEVVRVATKFPDTARRYFERMGRQAEIIELHGSIELAPLVGLAECIID
                     LTATGTTLRENDLVVLDEISTSTARLISNRGSYRLRHSEVIALADAMQRRGERFG"
     gene            27772..29016
                     /gene="hisD"
                     /locus_tag="VN178_04865"
     CDS             27772..29016
                     /gene="hisD"
                     /locus_tag="VN178_04865"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWS80334.1"
                     /translation="MVRKLDARGRGPAGIVGELRRPGGFLSQAVRDSARRIVEDVRDR
                     GDAALFEYTERFDGVRPGEIRVSKSEIDNARESLSPEVEESFGVAIENVRAFHENEMD
                     RPWERSHAGATTGQRVRPIRRAGLYIPGGHAPYPSTLVMSAVPAQVAGVGEIRVCTPP
                     GPDGSPDSDVLAVGGLLGLEEVYAVGGAQAIAALAYGTESVAAVDKIVGPGNDYVTAA
                     KLEVFGIVGIDAPQGPSEVLVIADAGAVPERVATDLIAQAEHLSGASAVLLTPSEHLI
                     SRVEPLLWGEPAEHVCLVLVEDLARAVEISNRYAPEHAHVISADHEAVMRDLDAAGMV
                     ALGDFSPVALGDYAAGPSHVLPSGGTATWASALGTHDFLARTNYIHFSRDALRDLGPH
                     VERLARLEGFDNHAASVETRMG"
     gene            29062..29646
                     /gene="hisB"
                     /locus_tag="VN178_04870"
     CDS             29062..29646
                     /gene="hisB"
                     /locus_tag="VN178_04870"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWS80335.1"
                     /translation="MTRKGEAERETAETFVRVSLELDGGGEAEVATGVGFLDHLLHLL
                     AHHSGMDLTVEAKGDTWVDDHHTVEDTGLVLGRAFDEALGERSSITRFADASAPLIEA
                     LSTAVVDLGARSHLTCNLGPMPEKIGTFDVELFPEFLRAFTQYGRFTLHLNCHYGENA
                     HHKIESGVKALAVALKEGIAPRTAGSASTKGVVD"
     gene            29866..30477
                     /gene="hisH"
                     /locus_tag="VN178_04875"
     CDS             29866..30477
                     /gene="hisH"
                     /locus_tag="VN178_04875"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWS80336.1"
                     /translation="MNVAIVDYDAGNTLSVTRALEKVGAKVDLTPDPERVLAADAVVL
                     PGVGAFGDCMRKLRERGMDEACREAFRRGKPFLGVCVALQVVFESSGESPGVEGLGLM
                     PGKVVRFEGHGLKVPHIGWNELSLVRDHPVISGLDGEAFYFVHSYYPEPEERRDLIGE
                     TDYGTRFCSVAGRENLVAVQFHPEKSSRAGLKLYGNFLAWAGT"
     gene            30482..31087
                     /locus_tag="VN178_04880"
     CDS             30482..31087
                     /locus_tag="VN178_04880"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWS80337.1"
                     /translation="MTEGERASGEAFLRGRRVWLRPLERVDLPRYQELFSDPEINLLY
                     GGLGVPENLAKLQRHYDAGEYDTTDRFLLLVIEAEGTIIGAIVLRNTENLPSRSATFG
                     ILIGDPAYLDRGYGSEASALLLDYAFAVLGHHKINLDLFEYNARAQAMYEKLGFVVEG
                     RRRENHWSRGRFWDEILMGVTAEEWWSKHGSPRLPGTAPAR"
     gene            31087..31827
                     /gene="hisA"
                     /locus_tag="VN178_04885"
     CDS             31087..31827
                     /gene="hisA"
                     /locus_tag="VN178_04885"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWS80338.1"
                     /translation="MVSQKRSFTVFPAIDLRGGRCVRLKQGDFGRAKEYDADPVERAR
                     DWERQGAKAIHVVDLDGAEEGRPVQLGLIRSIARAVGVPLQVGGGVRSIEDLRAVRSA
                     GARRVVMGTAAVEDRDLRLRAVQEAGDSLLVAVDAREGVIATHGWLRSSGVSVLELSK
                     ELVTEGVASVLYTDVSRDGMDAGAALDGTAAVAEIIPTVASGGVRGPEDVATLSSTPG
                     VTGVVVGTALYEGKTTLPDLLESVQGSS"
     gene            complement(31883..32191)
                     /locus_tag="VN178_04890"
     CDS             complement(31883..32191)
                     /locus_tag="VN178_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80339.1"
                     /translation="MPQLRCTVRRVGDHESRTEEYEVAYALGRATPRVLLIKYQAARA
                     YEINAALDAAVKVAREAIAEADPDQRGPTDEALSKFSWRTIQSAKYERGEPRSSSLDY
                     "
     gene            32278..34245
                     /locus_tag="VN178_04895"
     CDS             32278..34245
                     /locus_tag="VN178_04895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWS80340.1"
                     /translation="MNEFFETSKKKSKRRPPGRFVRGLRALFNLVLVLGVSAAVGIAC
                     LYLFVLREYESDLDRTYPELAENSYVYDVDGDKIGEIPVTERRETVGFEGLGAHLPGA
                     VIAVEDRRFYDHFGVDFEGLARAAWTDLRSWEVQEGGSTITEQLMKNLYVPEGQRFET
                     SFRRRFVQSALAFAYERKHTKNEVLTAYLNTVYFGDGAYGAEEAAQRFFGKSARDLDL
                     SESAMLAGLLHAPSTYVTWEGEVIEGQVRERRDSVLGLMQEQGMISSRQRDEAEDQPL
                     EFAPDPPPEDPAYTPFLEKVAREVEEELGPRALQLGGLRIYTTMDPDLQQAAVETSDD
                     VLSEPDDPSAAVVAIEPQSGAIRSLAGQDGDFNLPLDARRQPGSSFKPVVLAAAIKED
                     ISPESTYISQDLAFDFRDEYYEVHNYDYVERGEISISDAMEESDNTVFVQLAADVGLE
                     DVVKTAEDLGITTPVEPYPSTAIGGLGSGVSPLDMASAYSTFAGGGIHREPYSIERID
                     RNSYGQSETVYDHQISGRRVLTGNEAAVATQVLRGVVEDGTATMFHDLDEEIGRPSAG
                     KTGTTDDFVDAWYVGYTPLLCTSVWVGYAEGGRSLVGVHDLKEPNGETLPMDIWSDYM
                     ASATAGDLALEFPEADTAELYAVEGGYAGGF"
     gene            34250..34702
                     /locus_tag="VN178_04900"
     CDS             34250..34702
                     /locus_tag="VN178_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80341.1"
                     /translation="MDLQRLYAGSFIDWEAVAASWNKRTVPSRLLLFAARRYLDEGGG
                     KVESGRYEALSSAPLPEEIKRAFASPPELDAPAPRRWGEFVDAAVAAELEMVSYGERP
                     PLLHELRAGLEQAAAEADPLTEKGRWFLARRDALPGTDLPDDPGYLPI"
     gene            34942..36387
                     /locus_tag="VN178_04905"
     CDS             34942..36387
                     /locus_tag="VN178_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HWS80342.1"
                     /translation="MEAIPSASYSLTLRVEFPHEAGALGKILTTIGDADGMIGAVDIV
                     RMSQDRTTRDITVNARDSEHGQQVVQVVEDLPQVRVINVSDRTFLMHLGGKIEVRSKL
                     QIRTRDDLSMVYTPGVARVCRAIADDPERAFNLTVKRNTVAVVSDGTAVLGLGDIGPW
                     AAMPVMEGKAALFKQFANVDAFPICLDTKDTDAIVETVKNLSPAFGGINLEDISAPRC
                     FEVEERLKKDLDIPVFHDDQHGTAVVVLAALINSLKIVGKKVEDLSIVVNGVGASGVA
                     CAKIIMAAGAKNIVGCDSKGIVHEGREDLNESKRWFAENTNPEGRTGDLSDAIAGTDL
                     FLGLSAPDVLDLQQVGSMNTDPILFAMANPDPEIRPEVAMGKARIIATGRSDYPNQIN
                     NVLCFPGIFRGALDVRAREIDEPMKLAAAEAIANVIPQEDLSEDYIIPSVFDERVAPA
                     VAEAVAERAKETGMARRVQERAETGLTVTSF"
     gene            36534..36809
                     /locus_tag="VN178_04910"
     CDS             36534..36809
                     /locus_tag="VN178_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWS80343.1"
                     /translation="MSKLCQIFVCATPGEGRCGAKGGGELFEMFREEVELRGIPPSAV
                     LRNACTRRHEEGPVVFVYPDDVWYTRVTPEDVPEIVERHLAPLRVES"
     gene            complement(36806..38083)
                     /locus_tag="VN178_04915"
     CDS             complement(36806..38083)
                     /locus_tag="VN178_04915"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS80344.1"
                     /translation="MPERSVSIRRSARVVVLGAGFAGASLLRHLPPALRKPGETLLID
                     RREEYAFVPLIHEVAVGRVHPRTVTSPIAPLCAGDCVFLHAEVSGIDLARKTLETSEG
                     PVGYEYLVLNPGSVAAEPGPRLAGYFHSFWSLDDALRLRTALGGAWRLATSPGPRPPQ
                     GTLTVAIVGGGATGVEVAAEVAALFDYLGKRSHRRPSEEPRVVLLEAKHRLMDWLDPY
                     FDSVARKTLTGLGVEVRLNTPVETASAEGVFAGEDWLKAGTRVWTAGVEASPLVRGLP
                     GEHDAAGRSHVGEHLTLPGHPEVYVLGDAGVYTDPRLGPLPPTASVAVQQGPWVARDL
                     KRRLRGAGSKRGRPPFRFFDRGYVVSLGPESAVADAVGLRLRGPAAQALYRSVLLYYM
                     KSRRDRILTGADWAMERTLGRVGFGTQSPERAD"
     gene            38295..38603
                     /locus_tag="VN178_04920"
     CDS             38295..38603
                     /locus_tag="VN178_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80345.1"
                     /translation="MGLGNIAPGLIVRHTNRRLGKLDSYEACERLISGRISFSEFVDQ
                     VGEEYRELLDFMMDNDLHPGRPEEVLFCIDCHAGLYDRTLLRSQLEGLYSHDRSESIG
                     "
     gene            38768..40120
                     /locus_tag="VN178_04925"
     CDS             38768..40120
                     /locus_tag="VN178_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS80346.1"
                     /translation="MGTTLIILFVVVASILAASIVLGRDTREVESARDTEYLELEGVW
                     IRYNVIGGGPPVVLVHGWLSSTRIWEQLAGRLAQRFTVYTLDLPGFGESDKPLSGYGI
                     RNGSRLLYAFCAHFGLTHANVIGHDLGGAMAVKLAADHPDVVGRLVVICAPADEDQLD
                     LPTLLWLATVPVVGPIVYSLGRAARPFRRLWMRPFVADSDDLTEEIVDDAGRSTPAAV
                     SKTLSISRREISRGRLARQARIIKIPFLVVAGEEDQIVDPQSVGVWAEGVERAEICLI
                     DECGHMPMVERVAEFNAQILAFLTGDARYLDFVHPVAETKEGVEPARVEGGEVDYAAP
                     GPSDDTVDLSPRPTELESERDNVPRVVRKHGGRYPARNRPHQPPETYPDAVEEQPAPG
                     GRSRREPLDEDFLPDLPDDLFDWPETRRDFGSRERRQDGPSDGQDDDYPPGQENPPRS
                     "
     gene            complement(40117..40740)
                     /locus_tag="VN178_04930"
     CDS             complement(40117..40740)
                     /locus_tag="VN178_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80347.1"
                     /translation="MEAEQFTIRLARPDDDPAIAALVVEGFLDQFRHVFGGRMDRSLK
                     VMERWIRLEHTSGGVTSLVVEGYADGEIAGSIGVRTEVSREDILARGLWRALTRNLGF
                     PRAVWATTLLSYPTYSSVTSEAYIERLVISPSFRRQGMARRLLSAAEDLARDAGKKSV
                     GLHVTGNNLGALRLYEAEGYAEVSRQRSLIAAWFMGIREWLYLKKQL"
     gene            complement(40768..41565)
                     /locus_tag="VN178_04935"
     CDS             complement(40768..41565)
                     /locus_tag="VN178_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80348.1"
                     /translation="MGTIRIPSARGSFRVWQRNITIFRKYWKTILFPNFVEPLLYLAA
                     LGLGLGAFIQQGGINGQPYVQFVAPGLLASNAMFAASFESTFNTFVKLKIDKIYDAII
                     TTPVNAEDVVGGEYLWAGTRAALYGTAFLAMIVVLGIVFGQPLVTSWWALLLPLMLLV
                     IGIMFSVMGTLFTSLIERIDLYAYYFTLVVTPLFLLSGVFFPVDDFPAPVPQIAWFTP
                     LYHAVNVCRELATGPSLGVLVDVAWMLVFTGALALIPVQIMRRRLIG"
     gene            complement(41565..42530)
                     /locus_tag="VN178_04940"
     CDS             complement(41565..42530)
                     /locus_tag="VN178_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS80349.1"
                     /translation="MNAETERTKGALTGEPTLEARGLKKSFSGFEAVKGVDFEVFGGE
                     CFGFLGPNGAGKTTTMKMIYGAVIPTGGELAVAGLDVYHHEREIKRRIGVVPQENNLD
                     DELKVEENLLIYGRYYDLPSKLVRRRAEELLDFMQLSDKAEATVEQLSGGMKRRLLIA
                     RALLNDPEIVVLDEPTTGLDPQARLLVWDRLRELTEEGKTLILTTHYMEEAARLCDRL
                     VIMEGGLIIAEGKPGALVEEHVSPQVLEFRTDHRSLEDLVPVLEAVSDAVERTGDESI
                     LAYTDDADALLERVRDSGTEIENTVYRQAGLEDVFLRLTGRRLVE"
     gene            43025..43654
                     /locus_tag="VN178_04945"
     CDS             43025..43654
                     /locus_tag="VN178_04945"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWS80350.1"
                     /translation="MERAASVQVAGAPEAASEQKREDAQVLASEEEDKKTTAEKNEPA
                     QKPEPKKRSTIPTPPTNDLWMSIPALGLYDNYVTNSAAESAMDYGAIKLPHSGFPWQD
                     NANTYIAAHRLGWPGTASHHQFYNLPLLAKGDVIYLGDVNGTTYTYEVTEFKEITPDE
                     TWVTTPKPGRDMVSLQTCIETFGDYWTMGPNWYVRYIVQADRVSVDVAE"
     gene            43933..44961
                     /gene="fba"
                     /locus_tag="VN178_04950"
     CDS             43933..44961
                     /gene="fba"
                     /locus_tag="VN178_04950"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01521.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWS80351.1"
                     /translation="MALVTMRQLLDEAANGGYGVGAFNVNNLEQVQAIMEAARETGSP
                     VIIQASRGARKYTGDRFLYHLMEAATEIYPEISVALHQDHGNSPETCKSAIDLGFTSV
                     MMDGSLMEDGKTPADFEYNVNVTREVVEMAHASGVTVEGELGTLGGIEDDVGSGEVNL
                     TDPDQAVEFVERTGVDALAVAIGTSHGAYKFTKEPDREVLAMHIIEEINERLPTTHLV
                     MHGSSSVPKELVDIINAYGGEIKPTWGVPVREIQEGIKHGVRKINVDTDLRLAATGAI
                     RKAFAEDPSEFDPRYYLKPARAAMQEVVKARMQAFGQAGHATDYDPISLPDMAVRYKE
                     QGHHLTVT"
     gene            complement(45015..45680)
                     /locus_tag="VN178_04955"
     CDS             complement(45015..45680)
                     /locus_tag="VN178_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWS80352.1"
                     /translation="MVTQPSASVGDYLKAVWEIAGSGAASTKGVADRLSVSSASVTNM
                     FARLQGMGLALYEPYRGASLTRRGRAEALRLVRRHRLIETFLLEHLGYSWEDVHEEAE
                     RLEHAVSDEFTERLAELLGHPERDPHGAPIPASDGALPPEDSRPLSGTEVGGRFVVSR
                     VENRDPAVLIYLRGLGLVPGRALRVREVRALDGVVTAEDEDGVTHALGAPLARSILVR
                     HRA"
     gene            45844..46887
                     /locus_tag="VN178_04960"
     CDS             45844..46887
                     /locus_tag="VN178_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWS80353.1"
                     /translation="MDRSELGVSRRDFLRAAGVAGALGVSGSLLGARAALGHDGEKHG
                     ADHKAGHGAADSGGMAAHGGNGTVGDVDLSRFDPSKFLREFYWGEERTEGGRTVREYE
                     LSAEAVEVEVAPGVMYPAWAYNGQVPGPTLRAREGDLLRVVFRNRDAHPHTIHFHGFH
                     PAGMDGVFELVAPGQEFVYEFEAEPFGCHLYHCHVMPLKKHIEKGLYGAFVIDPREGR
                     EEADELVMVMNGFDTNFDGANEVYAVNTVAFHHQRHPVELKLGQLVRIYVVNVLEFDF
                     VNSFHTHANFFDYYPTGTRLEPSEFTDTKMFAQGERGIMEFSYDFPGLFMFHAHVSEF
                     AELGWMGLFDVKE"
     gene            46891..48066
                     /locus_tag="VN178_04965"
     CDS             46891..48066
                     /locus_tag="VN178_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWS80354.1"
                     /translation="MGRERGPVWVLLGLVPLLGLGVLLAAIVLNGAGIDREDRPPVED
                     LTIDRVSLPTPDEIVVSVTNGGPEPVTVSQVTVNEALWDFTVEPDARISPLGSAEITL
                     PYSWIEGEAYGITLITDTGTTFSAEVPAAVITPTFTAETFWRYALIGTYVGVIPVALG
                     LLWYPFLRRLGSAGIGAILALTLGLLVWLVVDTLLEAVEVAGRLPGVFSGVPLVLVVA
                     LLALLGLLGAERLFRRRGQTSRLSTSYRIALGIGLHNLGEGLAIGAAFALGEAALGTF
                     LVVGFTLHNITEGVGIAAPILEERPSLAHFAGLALLGGGPAVLGTWIGGFAYSNLASA
                     VLLAVGAGAILQVIYEVGRLLLKDSARSKTPALSVPNLGGFTAGVAIMYVTALLVSV"
     gene            complement(48123..48797)
                     /locus_tag="VN178_04970"
     CDS             complement(48123..48797)
                     /locus_tag="VN178_04970"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWS80355.1"
                     /translation="MVTRRTYDDGCAAAHALDLVGERWALLVVRELLLGPKRFTDLRA
                     GLPHASPNVLAQRLRELEEAGIVRRRRLPPPAASKIYELTEWGEELEPVIIRLGRWGA
                     RSPSIPGDAPLGVDSLILSFRTMFDPGAAEGLEASYELRMGEDRFRAEVSRNGFEVER
                     GGVERPDAIIEADSATLAGLVYQDRDLEEALRSGDVRVEGDEAAVERFLTLFPLPEPA
                     ARYSLF"
     gene            48934..50433
                     /locus_tag="VN178_04975"
     CDS             48934..50433
                     /locus_tag="VN178_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80356.1"
                     /translation="MTQYEFGKHEDSHTETMGGRRRWVALYVLCVGMLMIVLDATVVN
                     VALPAIQNDLGFSQSSLAWVVNAYLISFGGLLLLAGRLGDLISRRGMFLAGIGVFTAA
                     SLLCGLAQSQELLVAARFVQGAGGAMTSAVILGMIVTMFPEPREQAKAIGVFAFVASA
                     GGAVGLLVGGILTQSISWHWIFFVNVPIGIVTAALAGRLIEGDKGIGFGDGADVAGAV
                     LVTGSLMLGVYTIIGPAAEYGWGSGRTLTIGAISLALLAAFVAREATARNPLVPLRIF
                     RSPNITGANLIQVLSVAGMFGMFFLGALYLQRILAYDALQTGLAFLPVTILMGTLSVR
                     YTDRLVMRFGARKLVLGGLALFMTGLALFARAPVDGDYVVDILPVMVLLGIGAGLCFP
                     ALMTLAMSGATPSDAGLASGLINTTAQVGGSLGLAVLATLSASRSDQLIGSGESTAAA
                     LTSGYHLAFGIGAGLVVVAIAVALTVLQPERQTEEGIQTEAEPFDCEAA"
     gene            50477..51589
                     /locus_tag="VN178_04980"
     CDS             50477..51589
                     /locus_tag="VN178_04980"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWS80357.1"
                     /translation="MDDIRTQIQEAIDELVESGAERGLQVTVYRNGEQVVDAVAGVAD
                     HETGRLVESGTPIYCYSVGKGATSTVAHVLVERGLFGYDTPVVDLWPEFGAHGKETAT
                     VRHVLTNTVGVPGLPDDTTPEDLCDWDKMCAAIADAEPWWEPGTKIAYHAYPFGYIVG
                     EIVRRATGKPISQVLREEVAEPLGIADEIYFGVPESELGRLARLEDMEGSAQMLASIP
                     DDSPFFKLGPRQVTPTAEFGNRPDILMADIPAGGKMSARAVARMYAALVDEVDGVRFV
                     SPERLREISAGTFSGVDEIMGFPTAWALGYSVGGPGADPQETGSVLGVGGVGGSFACV
                     DTATGVSWALTKNRLTASFESAQQISEIVTKAVEED"
     gene            51676..52089
                     /locus_tag="VN178_04985"
     CDS             51676..52089
                     /locus_tag="VN178_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS80358.1"
                     /translation="MDWKLEVVTIPVSDIDRARDFYSEKVGFVVDIDFKVSDDVHLVQ
                     LTPPGSACSIHLGTSTTAMEPGSVDGVFLVVSDVLAARAELVERGVDVGEVQVFEEGA
                     YRPAREGENLDLVGCVFFSDPDGNRWCVQQIPARN"
     gene            52109..52729
                     /locus_tag="VN178_04990"
     CDS             52109..52729
                     /locus_tag="VN178_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS80359.1"
                     /translation="MGKVTSGLTMSLDGFIAGPNDGPEHPLGEGGMRLFDWYSSGDTD
                     YVVPTGEMTFQVSSQAAEMLNEVFSSIGAIVTGRRTFDITNGWGGRHPIGEGIPIFVL
                     THSVPDGWDYEGSPFTFVTDGVRSAVEQARAAAGEKNVAVGAASLVQQCLEAGLLDEV
                     HVDVVPVLLGDGVRLFDNLGTEPIELERTALIADPDVTHMTFRVLK"
     gene            52739..52975
                     /locus_tag="VN178_04995"
     CDS             52739..52975
                     /locus_tag="VN178_04995"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWS80360.1"
                     /translation="MSPQQESNLPPGLSRPARRALTNAGYRTLEQLARVSEKEIEDLH
                     GVGPKALQQLRHTLVANGLSFADGNRTNQQGTHE"
     gene            52972..53532
                     /locus_tag="VN178_05000"
     CDS             52972..53532
                     /locus_tag="VN178_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWS80361.1"
                     /translation="MKRQVLFVHGGGEGAYEEDRKMAASLREALGGGYEVHCPKMPHE
                     DSPQYSAWRDRVASELTGFDGEVFLVGHSLGASVLLKYISEERTATPISGAFLVAPPY
                     WGVEGWEVDEYALQEDFASKLPSGLPMFFYHSRDDEWVPFAHLALYAEKVPHASFREF
                     DGRGHQLDDDLSEVAHDIASLERTGS"
     gene            53558..54289
                     /locus_tag="VN178_05005"
     CDS             53558..54289
                     /locus_tag="VN178_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HWS80362.1"
                     /translation="MNESYANLPRVVSRDEWKAARKDLLAREKRFTRERDALNADRRR
                     LPMVRIEKEYEFEGPDGKASLLDLFEGRRQLIVGHFMFDPGWEEGCPSCSAGADEISD
                     GLLRHLHTRDTTFVYVSRVPLEKIEAYKASKGWTFPWYSSHGSEFNYDFHATLDESVA
                     PVEYNFQTIEDHDEQSAEVPGRSSFLRQGENIFHTYSVYARGLETVGGSYYFLDETAL
                     GRQEDWEEPKGRAADARGAVPDFSS"
     gene            54670..55452
                     /locus_tag="VN178_05010"
     CDS             54670..55452
                     /locus_tag="VN178_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWS80363.1"
                     /translation="MPRMDVMDAVVKLMEDEGVEVAFGVPGAAILPLYQALGKSGKIR
                     HYSVRHEEGGTQAADGYTRVTGKVGINIGTSGPAGTNMITGLYTCMADSIPQICITGQ
                     APTSVLHKEAFQAVDIVEIAKPVTKWAVQVKEPGQLVWTFREAFRIAQEGRRGPVLID
                     LPLDVQKSGLEVDFDPERGGPLRHIAKPEPRPGAIRRAIEMKPQRAFLEMNEFFDGDE
                     IIVTAIGLYQIFSGQFQKTYKPRHYLCCGQAGRIVGGVPERD"
     gene            55578..>56222
                     /locus_tag="VN178_05015"
     CDS             55578..>56222
                     /locus_tag="VN178_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWS80364.1"
                     /translation="MKDAVTTVMEQCDRLADISEEPGLIHRPYGSKAMHEVNDIVAGW
                     MRTAGMTTRRDQIGNLIGRYEGRGEKTFILGSHLDTVRDAGRYDGILGVMVAIACVQR
                     LHDRGERLPYSIEVVGFADEEGLRFGTSYLGSSAYAGSFDRERLLLEDRNGVTLREAV
                     RSFGGNPDAVERDGRSDEGLLGYCEVHIEQGPVLEKEDLPVGVVTTINGQSRIQV"
BASE COUNT        10549 a        17370 c        18429 g         9874 t
ORIGIN      
        1 ggaccatcac ggccgacgcc tcgccgatca ggaaccccga gtggtcgaca tcgtagggcc
       61 tgcacgcccc ctcggggtcg tcgttgcggc ggctcatcgc ccgcatggct atgaatccgg
      121 cgacgatgac gggtgccagg ccggcctccg aggcgccgca gagcactacg tcggcgtcgc
      181 cgcgccggat caggtcgtag cccaacccca tcatgtccgt gccgcaggtg caggcgagcg
      241 cgccggtgat cgagggtccc tggacgccga gctgtatgcc tacgtgggcc gcggcggagt
      301 tgggcatgat cttggtcacg gcgaaggggt ttacgcggga gggtcctttg ctatctatgg
      361 tcgcctgggt cgtctccatg ctggtcacgc ccccgatacc gctgccgagg atgaggccga
      421 cccgttccgg gttgttattg agcacgtccc aggcgtcagc atcttcgagg gccagcttgg
      481 ccgaggcgag gcccatctgc ccgaaccggt cggtcctggc cgccactttg cggtccataa
      541 agctctcggg gtcgaagtcc gtacactcgg cggcgatgcg cacctcgaag ccctcgggat
      601 cgaagagcgt tatggggccg gccccgctct cgttggccag gagcgagctc cagagcttct
      661 tgcgccctac tcctatgggg gtgaccgcac cgatccccgt tatgtaagcc tccgggcgcc
      721 tgtctgccat ctcgccccct agctgaatcc aagatccatc gaactacgaa tcttagcatt
      781 acacgcctcc ggcgtaactc gccctgtgct ataaccctca cgatgagtag gcggagggag
      841 aattgatctt ctacatcgcg atggtcctct ttgttgcgaa cttcgcgctc ggcatactcg
      901 tgcagtttcg catcgtggat acgaaacctt tccgctggct ccaccacgcc ctgtttttcg
      961 cggttttcgc ctccgccgcc gtcgcggtcg gcttcggttt ttggcagggc gcgccctacc
     1021 gttggatctt gttgcccgtc ctcgttctct tcgctttcct gccgcgggta cgggccggaa
     1081 cgccggggca cgccgcgctg gccagcgggg caatgatcct ctacgttacg ggattcgtat
     1141 ggatgcttta ggaggctgag attggatttc ctggaggtcc tcaagggacg caagactacg
     1201 aacggcccct tcctgccgga tcctgttaag cccgagcacc agcgcctgct cgtcgaggtc
     1261 gccgccatgg cgccttcgca cttcaacagc cagccctggc gcttcgtgct ggtcgacgac
     1321 cgcgagacca tagacgaggt tgcgaggata agcggcgaga gcatgacgcg ccttctggag
     1381 gaggggacgt tctggaagcg ctacaggccc tacttccgtt tttctgaggc cgagatggag
     1441 gagaaacgcg acggcatcca cattgaccag atgccgagcg tcctcaagcc tttctcgcgc
     1501 cagatcttct ccaacacggg ccaggccgtg atgaatcgct tcggcgtccc aaggaccctg
     1561 ggcgaggaca acaggaagct cgtcgcaggc tcgccactcc tcctggcggt cctactggac
     1621 aagacggagt accgtcccgg cgagctctcg ggtttctact ccatcttcgg gatgggggcc
     1681 gcggtcgaga acatctggct aaccacgacg gagcttggca tgggcatcca gttcgtctcg
     1741 acgcccatgg agatacccga gaactgggag cggctgaagc ggctgctgaa ggttcccgac
     1801 gacctggaac tgatggccgt ctaccggctc gggtacgtcc cggatgggca gcgaaggccg
     1861 acgatcgact ggtcgagccg ccagcgcaag cgtatctcgc agtatgtctt ccgcgacacc
     1921 tgcgagaagc cagagcccga cttcacaccg caggaagaac gttgacggta cccaggatcc
     1981 tctccgtcgc cagcgccctg ccgccccacc gggtcgggca agaggaagcg aagacgttcg
     2041 ccaggggcat gttctcgcac gggcgcggta acatcgagcg gctgctcccg atcttcgaca
     2101 acgtcaacat agagaaccgc tacttctgcg tacccgcaga gtggttcgag cgagaccact
     2161 ccttccccga gaagaacgcc ctctacgtcg agcacgccct ggatctctcc gagaaggccg
     2221 cgagacgggc cctggacagg gttggggcgc ggcctggtga ggtctgtgcg atctacttcg
     2281 tctcgacgac cggggtctcg acgccttcca tcgactcgaa gctgctcttc cgcctcggcc
     2341 tctcggagca cacgcggcgc gtcccgatct ggggtctcgg ctgctcggcg ggggcggccg
     2401 ggctcgcgcg cgccgccgaa cacgcccgcc tctatcccga ccaactggtc ctcctcgtcg
     2461 ccgtcgagct ctgtggcctg accttccagc ggggcgaccg ctcaaagagc aaccttatct
     2521 ccacgagcct cttcgccgac ggcgccgccg ccgtgctgat cggaggcgcc acgggccccg
     2581 agttactcgg tggttacagc accacctggc caggtaccga ggacgttatg ggctgggatc
     2641 tcgtcgagac gggcctgaaa gtgcagctct ccaagagcat cccgaacata gtgcgcgaga
     2701 agttccgcga caacctggag gaggcctgta attctctggg cctcgacttc gacgagctcg
     2761 aacatttcgt gctccatccc ggcggctcca aagtcctcga cgccttcgaa gaggcgctcg
     2821 gactgcaagc aggcgagctc gccacgagcc gggacgtgct acgcgactgc ggcaatttgt
     2881 cgagcgtcac agtactcttt atcctggaac gttttctcga gagcggcggg ctagcgaaag
     2941 gtgaccttgg tgtgctctcg gccatgggtc ccggattctc cgcggaacat gtctttttca
     3001 gatgctgacg gccgagggcc gtcagcatct ggggcgtcag gtttcaggct acgggcttca
     3061 ggtctttgtg gtgggcggag gatttgttga acgtcgtgtt gttggtcatg ggggtgatgc
     3121 tcgttggggt tcagcgcatc ctggagctcg ctctctcgcg gcggaacgag cggaggcttc
     3181 gggctcgtgg cgctgtcgag cgggggagcg gtcactatcc cgtgatagtc gcaatacata
     3241 ctctgtggct ggtctcgacg ctcgtcgagg ggcttctgcg cgggccggag ccacctgcgt
     3301 ggtggccagt gccgctggtg gcgttcctgc tcgtccagcc gttgcggttc tgggcgatcc
     3361 tgtctctggg ggagaactgg aacacgaggg tactcgtcgt gccgggtgga aaactcgtga
     3421 ggagggggcc ataccgctac ttcccccatc ccaactacgt cgtcgtagcc gtagaggtcc
     3481 tgaccttccc gctaatcttc ggcgcctgga taacggcgat cgtcttctct ctactgaacg
     3541 ccgccctgct ctacgtacgc ataaagacgg agaaccgggc gctgggggag ctggcaggat
     3601 aatataaagc tggtcagcaa gtcagcacgc cgcccggctt cgccggacgg cttgtcagct
     3661 agtcagcctc gctgtggata gcgacgggaa agtgctctgg agaagcgttg tctagggagg
     3721 gttggtccgc agccgcgcac gggtgacgaa ccgagatgct gacaagctga aatgctgacg
     3781 agcgaaggcc gcaaggcctg agcgtgctga aatgctaagc cgaccgtagg gaggcacaat
     3841 gctagatctc aaatttatcc gcgagaacgc gaaagcagta gaggagaaca cgaaaaaccg
     3901 aggagtcgag gccgacgtgg gcctggtcgt ggagctggcc gaccggcgct cggagctcat
     3961 tcaggagtcg aacgagctca agcaccgcca gaacgagatg gccaggagca tcggcaggga
     4021 gcgcgacgag gagatccgga cgaaactcat cgaggagtcg cgggccatga aagataggtt
     4081 gccgtccaaa gaaagggagc tgcacgaggt cgaggagcgg cttagggacg agcagtcgaa
     4141 gatcccgaac atgacgcatc ccgactcgcc catcggcaag gacgacacag agaacgtcga
     4201 gatccggcgt tggggtgaga tccccgactt ccccttcgag ccgaaggacc acgtcgagct
     4261 aggcgaaagt ctcggcatca tcgacttcga cgctggggcg aagaccacgg ggaccaagtt
     4321 ctactttctg cgcggcgacg ccgtgctatt ggagctgggt ctgatccgct actcgatgga
     4381 catcattatg gagcacgggt accagccgac cattacgcct gacctggcaa gggatcggat
     4441 gctggtgggg acaggatttg tgccgcgggg acctgagacc cagatctact ccgttgaaga
     4501 cacggacctt tccatgatcg ccaccgcgga gatcccgctc gcaggccagc atgccgacga
     4561 gataatcgat gagggagacc tccccctgcg ctacgcgggc ctgtcccact gcttccgtac
     4621 cgaggcgggt tcccacggcc gcgccagccg aggcctctac agggtccacc agttcaccaa
     4681 ggtcgagatg ttcgcgttca ccacgccgga gcagtcggag gacgtgcacg aggagatggt
     4741 cgggatagag gaacgaatct tccagggtct cggcctgccc taccgcgtgg tcgacatctg
     4801 caccggcgac ctcggcggcg ccgcctaccg caaatacgac ctggaggcct ggatgcccgg
     4861 ccgcaacgat ttcggcgagg tgacgagcac ctcgaacaca acggactacc aggcccgcag
     4921 actggccatc cgctaccgca aggaaggcgg aaggccccag cttcttcaca ccctcaacgg
     4981 cacggccgtc gccatgagcc gggccctggt ctccctactc gagatctacc agcaacagga
     5041 cggctccgtg atcctacccg aggtcctcgt tcactacgta ggcaaggcac gcctgactcc
     5101 cgcatgaccc tcacagcatc ctgttgtcgg cgacgctctt gggctacgtg ttcttccaaa
     5161 acttcaccca ccgtcaggcg gcgatccgca caggtatcgt cccaccccga atcgccattt
     5221 cagctatagg cgaaaacaac aagctgttaa gctgacgtgc tgacgagcga agcccgaagg
     5281 gctcagcgtg ctgactagct gaaatgctta cagagaggag tgtcgggatg acagaagcgg
     5341 tcgagaagct ggtcaagaag agcgaggacc cgagcaggtg gtatctgcaa ctcgtgcgca
     5401 tggccaagct cgccgactac gggcccgtgc gggggacgtt cgcgatcaga ccctacgggt
     5461 tcgagatctg ggagcgcgtc cagcgcgacc tcgacgaccg cttcaaggcc acgggtcaca
     5521 ggaacgccta cttcccgctg ctcatccccg agagctacct caagaaggag gccgagcacc
     5581 tcgagggctt cgaccccgag ctcgcgtggg taaccatagg aggcagagaa gagctggagg
     5641 agcggcttgc gatccggccc acctcagaga gcatcatctg cgatttctac aggaactgga
     5701 tccagagcta ccgcgacctg cccatcctta taaaccagtg gtgcaacgtc ctgcgctggg
     5761 agaaggtaac ccgaccgttc ctcaggtccg cggagttcct gtggcaggag ggccataccg
     5821 tccacgcgac ggcggagggg gcgcgcgagg agagcttgcg gatgctcggc gtctaccgcg
     5881 attgcttcta cgaggccatg gccattcccg tactcacggg cgccaagagc ccatccgagc
     5941 gcttcgccgg agccgtggag acctacacct gcgagggttt gatgggtgac ggacgcgcgc
     6001 tgcaggcagc gaccagccac gacctcgggc agaacttcgc ccgcgccttc gacatcacct
     6061 tcctcgacga gaatcaggac agggtctatc cgtaccagac ttcgtgggga ttttcgactc
     6121 ggacgatagg cggtttgatc ctggtgcatg gtgacgacag gggcctcagg ttgccgccga
     6181 ggatcgcgcc gacacaggcc gtcatcgtcc cgatctggcg cggaaagaac aagggagagg
     6241 tgcgccgcga ggccgaggct ctgcatacac aactgaaaga cttcggcatc aggatggaag
     6301 tcgacctcga cgaagagcac tcgccgggtt ggaagttcaa cgagcacgag ctcaggggcg
     6361 tgcccgtgcg ggtggaactc ggacccaggg acatcgagaa ggagcaggcc gtcctcgtcc
     6421 ggcgcgatac aggggagaag gagttcgtgg gcagaaaggt attgcccgag cggctgaagg
     6481 cgctattggg cgagatccag gcgaatatgc tgcggcaggc gacggagttt cgcgacgcga
     6541 acacccgcag ggccgaaagc tacgaggagt tcgaggagat catcgccaga gatcgtggtt
     6601 tcgtcgtcgc cccctgggac ggcatgaagg agaccgagca gaagatcaag gaagatacga
     6661 gggccacgat ccggctcctg cccttcgagc gggaggaggg gaaggacctc gtctctggaa
     6721 ggccgggccg caccgccatc ttcgcccgtg cgtactgaca gcatttcagc acgccgcccg
     6781 gctgcgccag gcggcttgtc agcatttcag ccagtcagct aggtggagtg tactgggacg
     6841 ctcgtgcgcc cgagataatg cccgaggagc atttactcca agagagtctg ctttcgacta
     6901 caagacctga agtgctgaca agcggaggcg aagccggtgc gggctgaaat gctgaattgc
     6961 ttgccgtgcg taaggctcaa gagccttacg cacggcgaca cgttgactcc aggatctaga
     7021 gcggttagca tttgcgcatt aagtgacata acgtaggtgc cagactctag ggcgcctgcc
     7081 tggagggggt ctccgtgcgg tcttccgtgc tatgcgcagc gaagtggtgt ctgtcgtctg
     7141 tcagggatag ggatggctgg cgagcgcctc ttgggcttct cgcgctgttg cctgttctaa
     7201 tcctgctggc cggagatcac atcgccgtcg aggccgaggt gtactccgcg tactcggatc
     7261 ccaagaagcc cgtcatcgcc gtcatcctct ccgagcggca gaacgtcgat gagttcaaag
     7321 cccatttcgg tctcacaggc gggcaggtcg acgccgtcct cgccgcgacc cgcctcgaga
     7381 atcggatcct ggcacaggag tatggggaga gcgagcgggt actcgccgcg aacaaagggc
     7441 ttcccaaaag agagcttgcg aaaaagatcg tcgcctccga ctacgacgag aaggtagcag
     7501 cggccgtggc agggacgaag aagaagatcg aggccatcgt gcccgaggat cagagcgccg
     7561 acctcgaggg ttgggtggac gagcagtgga accaggaagt tcaggccgct tccacggaag
     7621 actacgctgc cacctccgag tcctacatgg tctccagtct cagtaaaggc gtacggtgca
     7681 aagtcttcgc cacgcagtac atcgggtaca ccgagcgcga ggttgccctg ccgcatcgca
     7741 acctcaagct cggccgccag ccgagggttc gcatctggcg cgtcagcggc gggcccagcg
     7801 tggggccgag ggtcaaggag gtaggaccct ggaacaccta cgataattac tgggtgcgga
     7861 acgaggaccg aacgatgtac gaacgcgtac cgcgctgcaa gccacaggcg caggtcgcct
     7921 actaccagaa cttcaacaag ggtaaggacg agtttggcag gaaagtcctc aaccccgccg
     7981 gagtagacct gacaccggat gtcgcacgta gcatgggcct gaagaaatac cagaatgcct
     8041 gggtgtgggt acgctttccc tgggtccaca ggtaggaaag acccttacct ttctcctgaa
     8101 ggcgcgactt gcttgacgag gcgcacccct gtgttttagc atcaacacct agcgcgaacc
     8161 cgcatcgaga gcggcggagg gaactggccc cgagaaaccg cggcaaccca cggggagctg
     8221 tgagctactc ccgcaaggtg ccaagtccag caggacggtc attaccatcc tggaagatgt
     8281 ggggagaggt gggcagcctc ttctacgagc gggttgcgcg agaggcgtag aacccgatct
     8341 atagaaggag gccgcattac taccgagtat gacactagag ccgagggccg gagctagcca
     8401 ggccccggcc ctacgatccc accggatagg ctccttccag cccgaactcg gcggaagcct
     8461 gcgggacgtc gcccttgcct atgagacctg gggcgaactc aacgaagccg ccgacaacgc
     8521 cgtactgctc gtccacgccc tgacaggaga ctcgcacgcc gcgggagggc cctccgaagg
     8581 gtacaggcgt ggcggttggt gggacccgat ggtcgggcca ggtctgcccc tagacaccga
     8641 aaaatacttc gtcgtctgct cgaacgtcat aggaggatgc tcgggcagca ccggtccggc
     8701 ctccatagaa ccagggacgc ggcgacccta cgggatgcgc tttcccagtg tcacgatcag
     8761 ggacatggtc agggtacaga ggcgcttgct cgactccctc ggcgtacggc ggctggccct
     8821 cgtcgccggc ggttccatcg gaggtcagca ggcgctggag tgggccgtgg agtaccccga
     8881 tttcgtggag aaagccatac ccgtggcggc cacgggcgct ctcgggccgc aggggatagg
     8941 gatgagcgag atcggacgcc gcgctatcat ggccgaccct gactggcaag atggcgacta
     9001 ctacggcacg ggaaggacgc ccgatagggg gctcgccatc gccaggatgg ctgggatgat
     9061 gacctaccag agcgccgccg ggcagtggga gaggttcggc cggaggcccg ccgggcgtcc
     9121 cgcgctctac cccgagttcg gtgggaggtt cgagatggag agctacctac actaccaggg
     9181 cagggagctc accggccgct tcgacgccaa ctcatacctc tacctctcca gggcgatgga
     9241 cctctacgac gtggcaagcg gctacgagtc cgaggaagaa gcctactcgc gcatccgggc
     9301 cgaggtgctc ttcgtcggga tcacgagcga ctggctcttc cccgccgccg aagtacgagg
     9361 cgccaaagag aaagcgaaaa tggccggcgc cgaggcacac tacgccgaga tagataccct
     9421 gagcggacac gacgccttcc tgaaggactg gaaagaactc gaagaagcga tacaaccatt
     9481 cctagccaaa taagctgaag gctgagagct gaaagctaat ggctgggcga ccgaagggag
     9541 cgacaatgac agaccagaaa atccaggagc gccactacgg gttcgacacc ctggcattgc
     9601 acgccgggca ggaggtagac tcggccacga cggcgcgggc cgtacccatc taccagtcga
     9661 cctcctatgt cttccacgat accgaccacg cggctgacct gttctcgctc gccgaagagg
     9721 gcaacatcta caccaggatc atgaacccaa cgaccgacgt cttcgagaag cgggttgcgg
     9781 ctctggagaa gggtgtgggc gccctcgcga cggcctcggg ccaggccgcc caggggctca
     9841 ctatcctgaa catcatggga gccggcgatg agctggtctc cgcggccgag ctctacggcg
     9901 gcacctacaa ccagttccat tacacgctgc ccaaggtcgg aatcgacgtg aagttcgccg
     9961 actcgacgga tctcgacgcc atacgcgcgg cgatcacgga taggacgaag gccgtttatg
    10021 ccgagacggt agggaacccc gcgctcaaca ccctggatat cgagggggcc gccgcggtcg
    10081 cccacgacgc cggcgtcccg ctcatcatcg acaacaccct gccctccccc tacctggtca
    10141 accccctgga gcacggtgcc gacatcgtcg tccactctgc gaccaagttc atcggcgggc
    10201 acggcacgtc gatcggaggc gtcataatcg acggcggcgc cttcccctgg gacaacggga
    10261 agttccctgg cttcaccgaa ccggacccct cctaccacgg gctggagatc tacccgacgc
    10321 tcggcgagct ctcgtttatc ttgaaggccc gcgtccagat gctcagggac tggggccccg
    10381 ccctcagccc gttcaacagc tttctcttcc tgcagggcct cgagacgctg cccttgagga
    10441 tggagcgcca ctcgacgaac gcgcaggccg tcgccgagtt cctgcagggc caccccaagg
    10501 tcaactgggt caactaccct ggcctcgaag accatccttc gcacgagttg gcggcgaagt
    10561 accacaggga aggcctgttc ggtgcgatcc tcggattcgg catcgaaggc ggcctcgagg
    10621 cgggcaagcg ctttataaac gggctcgagc tgcacagcct cctcgcgaac gtgggcgacg
    10681 cgaagagcct cgtcatacac ccggcctcca cgacccactc gcagctctcg gtggacgagc
    10741 aaaaatctac cggcgttacg gatgactacg tcaggctctc ggtcggcctc gagtccatcg
    10801 acgatattct gtacgatctc gatacagcgc tgaacggagc gtgacctttt ggcgagacct
    10861 ctggtcataa agttcggggg cacttcggtg ggaggcggag cgcagttcgt ccgcgccgcc
    10921 agtatagcca ccgaggctgc caggaataac tccgtggccg tcgtcgtctc cgcgatgggc
    10981 ggcaccacag acaccctgct cggctacgcg aggatgagct cgccttgggt aaggcagacc
    11041 agaaaagacg agacacacga ggcccccgta gcggagcttc gtcggatgct ggcagagcgg
    11101 cacctgcgcg ccgcccgcga ggccgtggcc gccgagcatc ttcccggggt agaagggcga
    11161 gtggtcgacc ttctcggcga gctcgaagtg gtcctgggga ctcattccgg caacgtgaag
    11221 gcgcgccggg atgagatcgc cgtctacgga gagcgccttt cagcggagat cctggcgggc
    11281 gcgatcagta gccagggtat tccttccggg gtcgtcggtg ccgaccccat agcgacagac
    11341 acacgcttcg gggaggccga ggtggacccg gctgccacgc gcgagcgtac ggtccgctac
    11401 gtctcaccac tactggaggg aggcctcgtc gctgtcgtgc caggctacgt gggacgttcg
    11461 ccaggcgggt tgcccacgac gcttggcagg ggaggttccg acctctcggc gacggtccta
    11521 gggcgcgcgc tcgactccgg ggaggtctgg atcatgtccg acgtggacgg cgtgctggat
    11581 gcggacccgc gtctcgtccc cgacgcctcc ctgatgccgc gcctctctta cagggaggca
    11641 ggcatcttcg ccgggctcgg cgcgaaggtg ctgcatccca ggaccatgga gccagcctcc
    11701 gaggccggca tcgaggtctt cgtccgcaac actttcgcgc cggagggctc aggcacgcac
    11761 gttacgggct tcgatcccgg ggccggcgtc cgctgcgtgg cgctcaggca gaacgtggcc
    11821 atggagatcc cttgtacgga cggccgcgag ggaagggccg cgatggtcgt ctgcatcggc
    11881 tcgccggatg aagaggatct tgcccgcggc cggcgttgcc tgcgcgaggc gggcatcccc
    11941 gtgcagcact tcggcatcgc ggccgcgggg ctcgtgttcc tcgtgtccgc cgacgtgggc
    12001 ctcgaggcgc tgcgcgcgct gcactcctgc ctgatcccac ccgcaggaga cttcgccggc
    12061 gaagaggtag cgtgagacgg ctggagatag tacagctcgg cctggggcac gtcggtcggg
    12121 cggtcgccca gatcgtcctg gaagagcgca aacgctggct ggagcgccac ggtatcgaga
    12181 tgaggtaccg cgccgtctcg gatacctcgg gcgcgctggt cggcgaggag ttgctcccgc
    12241 aggcgatacg cctcaaggag gaaggcggca ccctcgccga acttggcgtc gagccgctgg
    12301 aaggcgtgct cggctccgaa cccgaaccag gcttcacccg ggtggtcgta gacgtggccg
    12361 tgcacggggg tactttcgac ctcgatgtgc ttggcataag gggcggagcc tttctcgtgc
    12421 tctccaacaa aggaccactt tcgggcagca cggcccagta ccgccagctc atgcgcatga
    12481 tcccggaccg tctctggcac gaggccacgg tcggtgccgg catgcccatc atctccacca
    12541 taggcggcct gcaggagacg ggggacgaga tactggagat acaggcgagc ccgagcggca
    12601 ccctcggctt cgtcatgagc gcggtcgagg gtggccgatc cttctcccag gcggtgaggg
    12661 aggcagtcga tctccactac gccgaaccgg acccccgcga cgacctctcg gggctcgacg
    12721 tggcccgcaa agccatcatc ctggcacgca tgatggggag ggagatcgaa ccacacgaga
    12781 tcccctacga gtcactcgtc cctcaaggcc tggaagacgt cccgctggcg gaattcatgg
    12841 agcgtctccc tgaacacgac gagtctttcg ccgcgcgcct cctcgccgtc gaagaacacc
    12901 acatgctccg ctacctggtc cgtatcacga agcatgggcc cgtaacggtc gggctgcagg
    12961 atacgcccgt ggagagcccc ttcggaccca taagcggccc cgagaacgtc ttcgatttcc
    13021 gcacgcgccg ctactcggat gttaccctca ctgtgcgcgg accgggcgcg ggaccgcaac
    13081 gcacggcgag cggcgtggtg tgggatttgc tggacatcgc gcacaaggct ttgtaggtgt
    13141 cagacttcag gtataaggtt tcaggtagtc tctggtggag gatcatatgg gtgatgggta
    13201 tacgttagcg gtagtcgggg ccgggctcgt tggggacagg ctggtcgccg agctcaggcg
    13261 cagagacttc ccactcagag aggtgcgcat cctggccagg agtgcccgta ccgcggtcct
    13321 cgcgggggag acgttccagg tcggcgtggc cgagccggag gcattcgaag gcgtggacat
    13381 tgctttcttc gccggtactg aaggcgagaa aggggctgct gtgcagctcg ccgatgaggc
    13441 catctcgcgc ggcgccgtcg tcatcgacaa tggcagcgac ttcaggctcg acccgagcgt
    13501 gcccctcgtc gtccccgagg tgaatgtggg cgcgctcgaa ggccaccaag gcctcatcgc
    13561 caatgccaac tgctccacca cccagatggt cgtcacgctc gcgccgctcg cgcgcgagtt
    13621 cggcctggtg aaggtcgtcg tctccaccta ccaggccgtc tccggcgcgg gacgcaacgg
    13681 cgtcgaggcg ttggagaacg gtagggaaga tgcgttccca aggcccatag tggggaacgc
    13741 cataccccag atcgggggct tcggcgacga tggatacacg accgaggagg cgaagatgag
    13801 ggaggagaca cgcaagatcc tctctctacc cgaccttccg gtcttcgcta ccgccgtcag
    13861 gattccggtc cacacgggcc acgccgagag cgtctacgtc gagaccgagc gggaggcgaa
    13921 tctccccgcc gtgctcgaag cgctggggac ggcgccgggc gtcgaggtca cggacgactt
    13981 cccgacgccc atcgaggtcg ccggagaaga tgggactcac gtcgggcggg tgcgcgtgga
    14041 ggggcgggtg attcaatact ggtgcgtcgc agacaacctc ctcaagggtg cggcgacgaa
    14101 cgcggtccag atcgccgagt cgctcgtcgg cgtccccgcg cgggcgtaga gtcgaggcgc
    14161 gggtcggtcc ttgccgccct gtacggagga cctgcgactc ggtaaactgc ggtgtgggaa
    14221 gttacccgcg gtgaggagag aggcatggca caaggcgaga agagatcctt ctgggacagg
    14281 ctgtttggcc gcaatcagcc ttccgcgcgt gaggagaggg tggtcgagta catcatccac
    14341 cgcctgggcg aaggggcgca cctgggcgac gtggtaaagg aggaatacgt gcggcgcaac
    14401 gcttcacccc tggagataga ggagatctgc gccgatccca ggctcgtcga agccgcgcgc
    14461 cagcacctga gacaggactt cgactcgggg gaactcgacc ccggcaagcg cccccgatag
    14521 ccccctggcg cacgaaacct gaaatctatc cagcctgtat aatctggatc tgcgagaacc
    14581 tgaattggag gctggatgcc gatctacgaa tacaagtgtg agaacgggca cgtcttcgac
    14641 gtgatgcaga agctgtccga agaccccctc tcgtcctgca tcgagtgcgg cgccccggtc
    14701 aggaaagtcc tccatcccgt cggcatctcg ttcaagggct cgggtttcta ctccacagac
    14761 tacagcaaga agggccctaa agaggccgcg cagaaagaca cctccaagtc cgccaacggg
    14821 gacagcgggt ctagctcaga agagaagaag ggcaaagaga agccggccga gtcctccacc
    14881 agctcaggga aaaaggactg aaaccatggc gcgcggcgcc gacctgcccc agagccgaga
    14941 ccgcagtgaa agtatcctca gaaaggcact ccgcgcggtg gtgctcggtg tcaccctcct
    15001 cttcgtcgtc tcagccctcg ccgtctcctg cgccctcgtc gtctacacag cctgggacca
    15061 cgatctcagc cacggcatct tcgccctcct cttcgccgtc ctcagcgccc agatagccat
    15121 taactggttt ctgtggaagg acgacgagga gcagtaggct tcaggctaca ggcatcaggc
    15181 tacaggcctc ggtggtatca gccgctctct gaagaccatc gaagacctca gtaaattctg
    15241 gcgtcgtctc cgccgatgcg cctgcaaacg gcggatacgt cacgatcccc agtcctgaaa
    15301 cctgtagcct gatgcctgaa acctttccct gaaaagtcct gcaaaacatg cctcaacttg
    15361 tggtcgaggt accctggaca acttgtaggt ccttgcctta tagtagccgg catcgggtaa
    15421 cgacggggag aacaggcgac cggcggcgct ctttttgggg cgtcgccgaa gcccgggagg
    15481 agagggagga aggttggcgg ttacgtcggg cgacgcattc gcgcatttgc actgtcactc
    15541 tgagtattcg atgctggatg gggcgagccg tatcggggac ctcatctcgt ttgcgaagga
    15601 tcaggggatg ccggggctcg cgctcaccga ccacggtgtg atgtacggcg cggtcaagtt
    15661 ctacaaggag gccagggcgg cggggataaa gcccctggtg ggttgcgagg tctacgtcac
    15721 ggccgaccgg aacgaccgga gtcgcgcccc ttactaccac cttacgctga tcgccaggac
    15781 cgccgagggc tacaggaacc taatgaagct ctcgacgtgt gggtttctgg agggcttcta
    15841 ctacaagccg cgggtcgata tggagatgct gaggaagtat gggcgtggga tcgtctgcct
    15901 ctcgggctgt ctttcggcgg aggtgcctgc cagaatactc gaggggcgtc ccgacgaggc
    15961 gcggagtctt ctgctggagt atgcggagat cttcgacgat gtgtatctgg agctgcagga
    16021 ccatggcatc gagcagcagc ggcgcgtaaa tgagggtctg ttcaagctcc acaaagagac
    16081 cggcattccg ctcgttgcgg ccaacgattc gcactacacc acgcgcaacg acgccaggat
    16141 gcacgacgtg ctgctgtgca tcgggaccgg caagttccac aacgacccca acaggatgaa
    16201 gttctcgggc caggagttct acgtcaagac cgcagaagag atggccagga tcttccccga
    16261 ccatcccgat gcgctggaga acacgatcag agtcgtcgag agcgtcgagg atgtggggat
    16321 cgagctcggc aagacgcgcc tgccgaactt ccccaagccc gagggctata ccgcggacca
    16381 gtacctgagg gaacagtgtc agagggggct ggtgaggcgt tacggtgggc gtgcgaagag
    16441 ccgtgaggtg cttggcaggc tcgagttcga gctgcagacc atcgagaaga tgggctttgc
    16501 ggactacttc ctgatcgtct gggacttcgt caagtacgcg aaggacagga agatcgcggt
    16561 cgggccgggg cgcgggtctg cggccggctc catcgtcgct tatgcgctgg agattacgga
    16621 ccttgatccc ctggagtact ctctgctctt cgagcgcttc ttgaaccccg accgcatcaa
    16681 catgcccgat gtggacatag atttttcggt ctcggggcgc tcggaggtga tgcgctacgt
    16741 cacggacaag tacggcggcc acgagcacgt agcccagatc atcacgttcg ggactatagg
    16801 cgcgaaggcc gccatcaggg actccggacg gatctaccag tatccctact cggacacgga
    16861 caagctcgcc aagttcatcc ccgacaagcc ggtcgggacc acactccgcg acgtgttgcg
    16921 ccccgagaat ggctcctacg tcgccgggga caaacacccc ggtcctgcgc gggagatcat
    16981 gaagttcgtg gggcaggatg aggccgccaa gcaggtcctc gacaccgctt tcgagataga
    17041 gggtttcgcc cgccacgcgg ggacccacgc cgcaggcgtc gtcatctcgg aggagccgct
    17101 caccgacata gtaccgctgc agcgcgtcgc cagggacgag ggggccgtca tggtccagca
    17161 tcccatgtcc gacgtggagg cgttgggatt gctcaaggta gatttcctcg gcttgaggaa
    17221 cctcgatgtg atcgaagaga ccctcgagac gattcggcgg ggcggcggcg aggagcccga
    17281 catacgttcg atccccctgg acgacgagaa gactctgagg ctcttcgcca ggggagacac
    17341 tttcggggtc ttccagttcg agtcgagcgg gatgcagcgg atgctccagg aagtcaggcc
    17401 cgaccgcttc gacgacctcg tcgcgttgaa cgcgctctac cggcctggcc cgatggacta
    17461 catccccaac ttcaagcgcg gcaagcacga ccctgagagc gtcgaatacc tcgacgagcg
    17521 actaaagccg atcctggagc ctacctacgg cgtcgccgcc taccaggagc agttgatgga
    17581 gatctccaag tctctggggg gatttactcc gggcgaggcg gacacgctgc gaaaggccat
    17641 aggcaagaag aacgccgcga tgctcgccac gctcagggac aagttcatgg ccggttgcgc
    17701 cgcgagcggc gtctctacgg acggcgccgg ggagctgtgg aactggatgg agaaggccgg
    17761 aggatactct ttcaacaaga gtcacagcgc gtgctactcg ttcctggcct tccagactgc
    17821 ctacctcaag gcccactatc cagaggctta catggcggcc ctcatgagca gcgtgatgaa
    17881 caccaaagac agggtgcccc agtacgtcgc cgaggccagg gcgatgaaga tacaggtcct
    17941 cccgcccgac gtaaatgaga gcgggcgtcg ctttacggtg gtcggggaga ccatccgttt
    18001 cgggctctcc gccgtcaaga acgtaggtga ctcttgcgtc gactcgatca tcgccgccag
    18061 ggaggatggt ccttttcagg acatcttcga cttctgcgag cgcgtcgacc cgaagaccta
    18121 caacaagcgg actctggaat ccctcataaa gtgcggcgcc ttcgacaacg tcgggccctc
    18181 gcgggcagcc atggtcgcgg tccacgccaa ggccgtcgaa cggtccacca ggggcctgaa
    18241 aggtgcgcac gaggaccagt tctcgatgtt cgacgtgtcc gagctcgcgc cgccgaggcc
    18301 agagtttccg gacgtcgagg aagaccagcg ccagagcctg gagtgggaga aagaggccct
    18361 cggcctctac gtctcggatc acccattgcg tcccgttctg cacaagctca gaaagcatac
    18421 tgatacgacg gtgtcggatc tcgaacttta caaggatggg gccatcgtgt gggtcggagg
    18481 gctggcgacc tcggtgcgga ccaacacgac gcgcaagggt gacatgatgg ccatgctgca
    18541 gctcgacgac acgcggggct tcgccgaggt catggtcttc ccacgggtct acgccaaatt
    18601 ctcggcgtgc gtgcgcgagg acgcggtcct caaggtcaag ggtaaggtcg agatgaagga
    18661 aggcatccct cgcatcgtgg cgctggagat ggaggagctt cacctcgagc ccggtgcgga
    18721 ccccgtgtac ctcgacgccg gcgcgttcgt cggccactca cagaatgtgg ccagagtggc
    18781 gttcgaactc ttgctgcgtc atcccgggga gagtccgatc tttctcgtct ccggcgaaga
    18841 gatggtcgaa gagaagatcg ggaacatcac ggactcctcc gacctgcacg ccgaactcaa
    18901 acgactcctc ggacccagat gcctctccta cacatctccc gccgagctgc cgatggagcg
    18961 ggtctcttga ccacgaccgt ggaagctcat actggttaga gaacggaggc ctcgtagact
    19021 tccggggtgg cagaagcgac cgatccaagg ccgaccgtcg aggacgcgag aggtcgggac
    19081 gtaaacctta cagaatcctg aacctgctcg ttcgtcctat tggtaggcgt gtccgggaga
    19141 acagggggtt gcgctgagac gcggggcggg cgatgatgca ggcttcccgg atctagaagc
    19201 ggaggctcgt gaacgtggca gataagatag tctttacgcg tggcgtccca ccgccggagg
    19261 cgttccccac cgaagaactg ggggcgtgct tggacgcggc ggtaagggag aatcccgacg
    19321 tcgtgttgca gtacggccaa cagcccggct acgcgccgct gcgcgaggag cttgctggcg
    19381 aatatggggt ctctccacag gagatcctga tcggcaacgg ttcgttgcag ttgcaggatc
    19441 tcgtctcctc ctatctcgtc gagcccggtg cggcagtcta tacggagcag cccagctacg
    19501 accgggcgct caagaccttc agacgtcgcg gggcgcgcat cgtggggatc ccgctggaag
    19561 aggacggcat aagcgtcgaa cggctcgagg cggccctagg acgcgaggtt cctgccttcc
    19621 tgtacctcgt gcccgacttc cagaaccccg cgggggcgac actctccctg gagaagcggc
    19681 agagagtcgt ggagctagca gaccgctatg gtttctgggt gatcgaagac atcccttatc
    19741 gcaagctgcg ctacgagggc gaggacgttc cgcttctgcg cgagatcgac ccttcgcacg
    19801 tcatcacgat gagctcgttc agcaagctcc tcagccccgg catgagggtc gggttcatga
    19861 tctcgccgga ggacctcata agggaggtga cggcgctcgg ggaggataca tatctctcgc
    19921 cggtccttcc cacgcaggcc gccgtcgccg agtacctgcg ccgtgggctc ctcggtccaa
    19981 acgtagagcg gttgaaagat ctgtacacac cccggtggaa ggcgatggca ggggcggtac
    20041 gccgcgagct gcccggcgcc caggcgttca tcccctctgg tgggttcttc gtgagcgtga
    20101 tgctacctga ggacgcgaac accgagaacc ttgtagggcg ggcaggggac atcgggctcg
    20161 tgctcacgcc gggggcggca ttcttcgcag atgcggagga aggagacgtg gccgacggcg
    20221 gcaggttcgt gcgcctgccc ttctgtgccg ttacgccaga tcagatagaa gagggagtaa
    20281 ggcgtctaac gagtttactc tgacgatgac taaagcggcg cgaaaaggtc gacctggttg
    20341 ccgtccgggt ccttgacgga agcgtaccgc tgtccccaga acgcgtccca ggggactttg
    20401 tgcccgaagc cgagggcggc gagttcctcg tacttcgcgt cgacgctcgc ggggttgggg
    20461 cagaggaagg cgagcgctac gcggtggcct ccggagggct ctgtccactc gggatcgatc
    20521 ttttgtatga gctcggcggt atcccacgcg acgcgtaggc cggacggtgt cgtcacctcg
    20581 gcgtgcggtt cgtcctcagc gccctccggg atgtccaggc cgagccgacg gtagaactcc
    20641 atcgaagctc tcatatcgcg cgcgacgatg ccaatcaggt ccaggtgtag atccactact
    20701 tcctccttgt cggtctttct gagcgtacag gactgcgggc gtcttaccaa ccggtgatcg
    20761 ggcgtcctac gctgtgcgca gaggtctaca acgtccccat gaagagcaca cgtcgttcat
    20821 gtaacatgga agctgtagtg gtaaagggag gcctcaaggt ttcggccggc gggcagggga
    20881 ggtattgctg atggacgagc aggtaacgca cgctagagag catgcggatc tgaaacgcgg
    20941 catcggcccc tggttgctgt tcttcttcgt cttgggcgac atcgtggggg ccgggatcta
    21001 tgcgctggtg ggcgaggtcg gggccgttat cggcggcgcg atctgggttg cgtttctgtg
    21061 tgccttcata ctcgcgatct tcaccgccgc ctcctacgcc gagctggtca ccaaatatcc
    21121 tagggctggt gggtctgcga cctacgtcaa caacgccttt cggaactctt tcgttagctt
    21181 tatggtggcc ttcgccgtgg cggcggccag cctcacttcg gcgagtgttc tgagcctggc
    21241 cttctcgggc gactacctcc tgcagttcat aaacgtccct agcgtactcg ccgcgctcgt
    21301 tttcgtggtg gtgatcgggt tcatcaactt ctacggcatc tccgagtcgg tcaggctcaa
    21361 cctggtcttc acgtgcgtcg agatcaccgg cctggtgctt atagtcgtga tagggttcgt
    21421 ggcactcggc gccggcacgg gcgagccggc cagggccttg gagttcaagg agggcacgag
    21481 catacttcct gccatcctgg cgggcacggt cctttcgttc tacgccctga tcggcttcga
    21541 cgactcggtc aacgtggccg aggaggtgcg ccatcccagc aggatctacc cccgcgcgat
    21601 cttcggcggc ctgctcgccg ccggtgtcat ctacctgctc gtcactttca ccgcctcgat
    21661 ggtggtgccg acggccgagc tcgccgcatc gagcggaccc ctgctggagg tagtggagag
    21721 atcgcctctt gcgatcccga ccaaactgtt cgcgctcatc gcgctcatcg cggtctccaa
    21781 cggggcgctc atcaacctga taatggcctc cagggtcatc tatggcatgg gggaccagga
    21841 tgtcttgccc gaggttttct ccagggtcca ccctagtcgc cgtacgccgt gggtcgcaat
    21901 cctcttcgcc acgctcgtgg tcttcacgct actcgccacg gtcggccgca accccaccgc
    21961 gctcgacacc ctctcgacca ccaccgtggt gctcctcctc ctggcgttca ttatggtgaa
    22021 cgtctccgtc ctcgccttgc ggagcgacga ggtcgatcac gagcacttcc gcaccccaac
    22081 cgtattcccc atcctcggca tcgtggtcgc ggcgactctc ctcatatata ccgccataga
    22141 cgactggacc gtattcgggc ttgccggcct gttgcttggg attggcctca tcctctatgg
    22201 cgtgaacgtc cttgtgaaga gaagcctggg ggaatctctg cagagcgggg agtagcccga
    22261 ccccatctgc ttccctgatg gccaaagagg gctgacgccg gggagaagat ctcgcgccgc
    22321 cgggccgatt acgccccgtg cggcgtactc tattgtggga gcgcgaagga acgtggcatc
    22381 accagtcgaa accgtcgcgg catatggcaa tggagctttg ggtacatcag aatggatgga
    22441 cagagtacct tggataatgg ggagggcgcg atgaacggtt tgctcaaggg ggcgcttgct
    22501 gcgggtggcg gggcggtcgg tgtcgaattg ttacggcggg ctttgttctc gggggcgcgg
    22561 cggaggtacg aaccctggga gagggcgccc tacaaagact ttccgaacag ggtgctcatc
    22621 gtcggtggcg ggttcgccgg ctacaaggcg gcccagtccc tctgcgagct cgccagggat
    22681 cgcgacgacg tcggggtcat gctcatctcg cgggagaact acttcacatt ctggccgatg
    22741 ctggccagcg ttatcggtgg cgacgtggcg acccagaacg tcgcccagcc cttgcgccgg
    22801 accctgatcc ggctcggcgc cagcttcagg cgggctcccc tggacagcgt cgatctgaag
    22861 aggcgatgcg tagtagcagg cggcaaggag ttcccctacg agcatctcgt gctctcgctg
    22921 ggggccgagc ccgcctactt cggcataccc ggcgtggagg agcactgcat aagcataagg
    22981 gggatcggcg ccggcgagac gatcgtggac agggtcatcg agcgctacga ggaggtgacg
    23041 ctggcggggg gcgagatccc cgactccaag ctgaccttca tggttatcgg gggcggggcg
    23101 accggggtcg aggtggcctc ggaactgcac agcctcgtcc gggatttgct cgtgcccgac
    23161 taccccaaca tcaatccgca ccgcgtcaag ataatcctcg tcgacgccaa caaggagatc
    23221 ctcaaagagc tcgaccccgc cctgaggagg gtcgcgcgca aaaagctggc cgacctccag
    23281 atagaggtga tcaacaacgt caaggcgaag aaggtcacgg cagaccgggt gatcctcggc
    23341 gacgggcgcg agatcatggt cgagaacgtc atctggacgg cgggctccag ggcgagcgta
    23401 aagctcgacg agatcgacct tccccacgac gagcggaggg gccttacggt ggaccgctat
    23461 atgcgggtcg ttggccacga caacgtgtgg gggatcggcg actgcgccgc gaccgttgac
    23521 gaggacggct cgcccgtgcc cccgaacgcc caggccgcag tgcaggaggg cgatgccgta
    23581 gcaaggaaca tcctcgccgt tatagacgga ggggagccga gacccttccg ctacaagtcg
    23641 ctcgggcaac tagtcgagct agggagcgag ttcgccgtca acgacgtcct cggcgtaaag
    23701 ttcagcggcc agttggcggc cctcttctgg cgcctgatat acctattcaa gctggagagc
    23761 ccggagagca gggccaggat cgccgcggac tggctcctgg atatcttcta cgacccgccc
    23821 gtcacccaga ttcggcgcta accgcgtgcg ccgatagaaa aaccatacct tcccgatatc
    23881 ctggccccag gtggccactc gattgtatca ttgtatacat aaccttccac ggctctctgg
    23941 taaaaaggct tctctggttg ggaaaggagg agctgggatg cgggccgtgt catgcgccct
    24001 tctgtgcaac tgccgttacg ccttgagggc cgacgacgac gaacgcctgg tagctgtggc
    24061 cctcgagcat ctgagacgat accatcccgt cgcgcctgtc ggggaggaat gggtcaggga
    24121 gatcgtcact gcccgcgctt acgagatcga gtacgccgcg gtgtacccgg gcaatctcgg
    24181 gaccgaggag gtctagtccg ggcgctatcg gcccggtagg atggccgcgt cttcgagcgc
    24241 aagccctggg atctcggcct ggcgcggcgc ctaacgcacc gagaacggcc cagccattgc
    24301 gttcttgggg atattctttc acccgcgaag atgctatgct gttattctgg gagcgtatcc
    24361 ggggcggggg taagatcacc ttttctcact tccgtctcgc caggaggggg tgttcggcat
    24421 gagaagaact gtcgttctgt cggctgcggt ggcctttgct gtgctgctcg tgtcgggcgt
    24481 ctggggcagt gccgccaggg aggccgtggg ggctaccgcc aaacccaata tcgtcttcat
    24541 cctggccgat gacatgcgca aagacgacct caagtacatg ccgaagaccc tctctgtatt
    24601 gagggacaag ggcatgagct tctcgaacgc cttcgtctcc aacgcgcttt gttgtcccag
    24661 ccgggccacg atcatgaggg gccagtacgc ccacaatacc ggggtctggt ccaacaaggg
    24721 ttcgaggggg ggctggaagg cctacagtag caacggcaac gagaaggaca acatggcgac
    24781 gcgtctggat gcggcgggct acaggacggg tctcttcggc aagtacttga atgaataccc
    24841 cggcgccaag gacaaaccgc caggctggga taggtggttt gtgcacacgg acggctccag
    24901 ttactacgac tacaagatca acgacgatgg caagatcgtc cgctacggct cggcccctgc
    24961 ggactacgaa acggacgtga tagcgcgtca cgcagcggcc ttcatcggtg cctccgcgat
    25021 ggcgaggaag cccttcttcg ccttcgtggc gccaagggcc cctcacagtc cggctacgcc
    25081 ggcaacgcgc gacgagcacg tccacgacgg cctcaaggcc ccgcgcctgc cgtcttttga
    25141 cgagaagaac gtctcggaca aacccccctg gataaggaag ctgccggggc tcagcgatgc
    25201 caggaaggcg cagatagaca accgcgccga aaagcgggcc gaaacgctgc aggcgctcga
    25261 cgacctcgtg gccgggatcg tgggcaggct gaatgacaga catgtgctct ccaatactta
    25321 catcttcttc acctcagaca acggatggca tggaggcgag caccgcatac cgaaggaaaa
    25381 atggcgccct tacgaggagg acgtccacat gccgctcctg gtacgcggcc ccggcgtgga
    25441 ggcgggccgc caggcccaca agctggtact cgacaccgac tacttgccaa ccttcaccga
    25501 tctggcggac acgaagatgc ccgactacgt cgatgggcgc tccctcgagc ctgtgctcga
    25561 ggggaacgcc accgcctgga ggagcgccat cctgctcgaa gcttcaggcc gctactcgcc
    25621 ggcctacaga ggaatccgca cgagcgacgg gcgcaagtac gtggagtacg cgggcggtgg
    25681 gaaggagttg taccatctcg gccgcgacgc ctacgagctg cgcgacacat acgatcccaa
    25741 gaacccaccc gtatccctgg caaagcgcct gaagacgttg gtgggttgcg atgctgctgg
    25801 ttgccgggaa gccgaggacg ggcagtagta gcgatctggt cgcagggacc gccaaggtag
    25861 tgtccatcgg ttaagatagc gtctcttatg gatcgcaaga cgcccaggaa gttcgccacc
    25921 acgcccggca cgcgcgatgt gctgcccccc gaatccacgc gactgctcga tgtgcaggga
    25981 aggatcaggg accggttcgc cctgttcggg ttcagggagg tcctcactcc tgcgctggag
    26041 tacgccgagg tgatcgagga acacggcctc agggacgtct cctacaagct cttcgaccag
    26101 gacaaccaga tgctcctcct caggccggag atgaccacac ccatagcccg cgtcatctcc
    26161 cagcgcctgg cgggcagccc gccgccgcac aagctctcct acgttctccc cgcctaccgc
    26221 cgcgctagcg tgggccgcgg gcagagcgcg gagctctacc aggccggcgt cgaggtcgtc
    26281 ggctcggcga gtccaacaga ggacgccgcc accatcgcgc tcctcgtcga cgtgctgcag
    26341 agcctcggtc tcaaagcccc ctcggacttc gccgtcgtgc tcggccagtc gaccttctac
    26401 gcgggctacc tgcggcacgt cgtccccgac gccgcaacgg ccgtaaccgg cgccctggcc
    26461 gcgaaggacc tcgtggccgt cgacgacctt gcggaggaac tgccggcgga ggccgccgcg
    26521 ggggtccgtg gcatcccgcg tatcgttggt ccggccgcag ataaatccct catcgaggag
    26581 gccggacgtt tcgcgctggg aagcgaggcg agggaggccc tcgagaacct gcgggagatc
    26641 ctgcgtcacc tcgaggcgca cggggcgctc ggggcggtta tcctggatct cggtctctca
    26701 gggcgccacg agtactacac gggcgcggtc tacgaggcct acgccgccgg tctcgggttc
    26761 accgtagcca acggcgggcg ctacgacaac ctactcaggt gcttcgggaa ggagctgccg
    26821 gcgacaggct tcgccatcta cctggagagg ctgctctctg ttttgcccga ggagggtccg
    26881 cagcccctgc tcgtcctcgt cggcggggac gtcggcggca tcgaggcagc ggcccgcctg
    26941 cggaggctag gcgtgcccgt gttgcatctt tcagaagacc ttccgcccga ggccgcggtg
    27001 gagtacgcca ggagcgtgga ggcggcgtgg atctcctacc cgacaacggg cggcgtcaag
    27061 ctcgccgccg ccgaccccgc cggagagttc gcttccacga gcttcgaatc cgtggcgagg
    27121 acggtgctcc catgagtgtg ttgcgcgtcg ccgtgcccaa gggcgccatc ttcgttgatg
    27181 cgctagcggc gctggagagt gccggtctcc cggctggcgt tctccgcgag aacgggcgga
    27241 ggctcgtcta cagggccgaa ggtaccgagt ttatcgtcag ccggccctcc gacgtgcccg
    27301 tcttcgtcga gtacggtgcc gcggacatcg ggatcgtcgg taaggatgtg ctcgatgagc
    27361 agaggcccaa cgtggtcgag ctgagggatc ttgggacggg gaggtgccgt atgatcctgg
    27421 ccgcaccaga gggcggggca gaagacgtgc gacgggcgat ccgccacgcc gaggtcgtca
    27481 gggtcgcgac gaagtttccc gacaccgcga ggcgctactt cgagcgcatg ggccgccagg
    27541 ccgagatcat agagctgcac ggctccatag agctcgcgcc cctggtcggc ctcgcggagt
    27601 gcatcatcga cctcacggcc acgggcacga ccctgcgcga gaacgacctc gtggttctcg
    27661 atgagatctc cacctcgacg gcgcgcctga tctccaaccg cggttcctac cgcttgcgcc
    27721 acagcgaggt catagccctg gccgacgcga tgcaacgacg cggcgaacgc tttggttaga
    27781 aagttagacg cccgtgggag aggaccggcc gggatcgtcg gggagttgcg ccggcccggt
    27841 ggctttctct cccaggcggt ccgcgactca gcacggcgga tcgtcgagga cgtgcgcgat
    27901 aggggagacg cggctctctt cgaatacacc gaacgcttcg acggcgtccg gccgggggag
    27961 atccgggttt caaagagcga gatagacaat gcccgtgaat ctttatcacc ggaggtcgaa
    28021 gagagcttcg gagttgcgat agagaacgtg cgcgccttcc acgagaacga gatggaccgc
    28081 ccttgggaac ggagccacgc cggggcaacg acgggacagc gcgtgaggcc catccgtcgg
    28141 gcagggctct acatcccggg aggacatgcc ccgtatccga gcacgctcgt tatgtcggcg
    28201 gtgccggccc aggtagcggg agtgggggag atcagggtat gcacgccgcc ggggcccgac
    28261 ggatcgcccg actccgacgt tctcgccgtc ggtggcctgc tcggtctcga agaagtctac
    28321 gccgtcgggg gtgcccaggc catagccgcg ctcgcttacg gtacggagag tgtggctgcc
    28381 gtggacaaga tcgtgggtcc gggcaacgac tacgtcacgg ccgcgaagct cgaggtcttc
    28441 gggatcgtcg gtatcgacgc gccgcaggga ccgagcgagg tgctggtcat cgccgacgcc
    28501 ggtgccgtac ccgagcgcgt cgccaccgac ctgatagccc aggccgagca cctctccggg
    28561 gcgtcggccg tgctgctcac gccgagcgag catttgatct cccgcgtcga gccactcctt
    28621 tggggggagc cggcagaaca cgtctgcctg gtgctagtcg aggatctggc gcgggccgta
    28681 gagatctcca accgctacgc ccccgagcac gcgcacgtga tctcggccga ccacgaggcc
    28741 gtcatgcgag acctcgacgc cgcaggaatg gtcgccttgg gggacttcag ccccgtcgcg
    28801 ctcggcgact acgcggccgg tccttcccac gtcctgccct ccggcgggac cgccacctgg
    28861 gcctccgccc tcggcaccca cgacttcctc gccaggacga actacatcca cttcagccgg
    28921 gacgccctgc gagacctcgg gccccacgtg gaacgcctcg cccgactgga aggcttcgac
    28981 aaccacgccg cgagcgtcga gaccaggatg gggtagacgt tgtccgttag aatgcttggg
    29041 ctctagcgat ccgggaggaa gatgacgcgt aaaggcgagg ctgaacgaga gacggcggag
    29101 acgttcgtca gggtgagctt ggagctcgac ggcggggggg aggccgaggt cgcgacgggg
    29161 gtgggatttc tggaccatct cttgcacctg ctcgcccacc actccggcat ggacctcacg
    29221 gtggaggcca agggcgatac ctgggtggat gaccaccaca cggtcgagga cacgggactc
    29281 gtgctcggac gcgccttcga cgaggcgctc ggcgagagaa gctccatcac gcgcttcgcc
    29341 gacgcgagcg cgccccttat cgaggcgctc tcgacggccg tcgtcgacct cggagccagg
    29401 agccacctca cgtgcaatct cggcccgatg ccggaaaaga tagggacctt cgacgtagag
    29461 ctcttccccg aattcctgcg cgccttcacc cagtacgggc gctttacgct ccacctcaac
    29521 tgccactacg gtgagaacgc ccaccacaag atagagtcgg gtgtcaaggc gctggccgtc
    29581 gccctgaagg aaggcatcgc cccgcgcacg gcgggcagcg catcaacgaa aggagttgtc
    29641 gattaagcat gtcagcacgg cgcctttggc gcctcgtcag cacgcttcgt gcctttcggg
    29701 ccctcgcttg tcagctagtc agcttcttcc gttcgggtct cgcccggcag cgcacgacga
    29761 accgccacca gcggcagtcg gttacgacgc tcgtggagag cccgcgaaac gactggctga
    29821 attgctgaga gccgccgacc ggagggaggc ggcgcgctga ctagcttgaa cgtcgcgatc
    29881 gtcgactacg acgcggggaa tacgctatcc gtcacgcggg cgctggagaa ggtcggtgcg
    29941 aaggtggatc tcacgcccga cccagagcgg gttctcgcgg ctgatgccgt ggttctgcct
    30001 ggtgtagggg ccttcggaga ctgcatgagg aaactgcggg agcgcgggat ggacgaagcg
    30061 tgccgcgaag cgttccggcg agggaaacct ttcctcgggg tttgcgtcgc gctgcaggtg
    30121 gtcttcgagt cctccgggga gtcaccgggg gtagagggac ttggtcttat gccggggaag
    30181 gtggtgcgct tcgagggcca tgggctcaag gttccgcaca tcggttggaa cgaactgtcc
    30241 ctggtgcggg accaccccgt catcagcggt ctcgatggcg aggcgttcta ctttgtccat
    30301 tcttactacc ccgaaccgga ggagaggcga gacctcatcg gcgagacgga ctacggcacg
    30361 cgtttttgtt cggtggcggg cagggagaac ctcgtggccg tacagttcca tcccgagaaa
    30421 agcagcaggg cgggactgaa gctgtacggg aacttcctgg cctgggcagg gacataaggc
    30481 gatgacggaa ggagaacggg catcgggtga ggctttcctc cgcggtcgcc gcgtctggct
    30541 gcgtccattg gagagggtgg atctcccacg ataccaggag cttttctccg accctgagat
    30601 caatctgttg tacggtggac tgggtgtgcc ggaaaacctg gccaagctgc aacgccacta
    30661 cgatgcaggc gaatacgaca cgacggaccg gttcctcttg cttgtgatcg aggccgaagg
    30721 caccatcatc ggggcgatag tgctgaggaa cacggaaaac ctcccgagcc gctcggcgac
    30781 gttcggaatc ttgatcgggg acccagcgta cctggaccgg ggttacggca gcgaggctag
    30841 cgccctcctg ttggactacg cgttcgccgt attggggcat cacaagatca acctcgacct
    30901 tttcgagtac aacgcccgcg cgcaggccat gtatgagaag ctgggcttcg tcgtggaagg
    30961 acgtcgccgc gaaaaccact ggtcgcgcgg acgattctgg gacgaaatcc tgatgggcgt
    31021 caccgccgaa gagtggtggt cgaaacatgg gtctccgcgg ctacccggta cggcacccgc
    31081 acgctgatgg tatcgcagaa gcgttctttt acggtctttc ctgccatcga cctgcgcggc
    31141 gggcgatgcg tgcgtctcaa acagggggat ttcggacggg ccaaggagta tgatgccgat
    31201 cccgtcgagc gggcgagaga ctgggagagg cagggggcta aggcgatcca cgtcgtcgac
    31261 ctcgacggcg ccgaggaggg caggcccgtc cagctcgggc tcatccgcag catcgcgagg
    31321 gcggttggcg tcccactcca ggtgggaggg ggcgtacgct ccatcgaaga cctgcgcgcc
    31381 gtgcggagtg ccggcgcccg gcgcgtcgtg atggggacgg cggcggtcga ggacagggat
    31441 ctgcggctga gggcggtcca ggaggccggc gattctctac tcgtggccgt cgacgcccgc
    31501 gagggagtca tagcgacgca cggttggctg aggtcgagcg gggtctcggt cctggagctc
    31561 tcgaaagagc tggtcaccga aggcgtcgcc tcggtcctct acaccgatgt ctcgcgcgac
    31621 ggcatggacg cgggagcagc cctcgacgga accgccgccg ttgcagagat aatccccacc
    31681 gtcgccagcg ggggcgtgcg cggtcccgag gatgtggcca cgctctccag tacgcccggt
    31741 gtaaccggcg tggtagtcgg caccgcgctc tacgagggca agacaacgct tccagacctg
    31801 ctagagagcg tccagggctc ctcatgacaa tcatcgtgaa acccgatgcc cttaggggcg
    31861 ggtttcaaac ccgcccctac attcaataat ctaggctgga cgagcggggt tcgccccgtt
    31921 cgtacttggc ggattggatc gtacgccagg agaatttgga gagggcttcg tccgtgggcc
    31981 ccctctggtc ggggtctgcc tcggcgatgg cttccctggc caccttcacc gcggcgtcga
    32041 gggcggcgtt tatctcgtag gccctggcgg cctggtactt gatgagcaac acgcgcgggg
    32101 tcgcccgccc cagcgcgtag gcgacttcgt actcctccgt gcggctctcg tggtcgccga
    32161 cgcgcctgac cgtgcaacgc aactgtggca tgtaggcatc ctaccatctc caaagccgca
    32221 cctggccccg cgtcgatctc cccagacacg tttggggtag actacggccg gagggtcgtg
    32281 aacgagttct tcgagacaag caaaaagaag tcgaagaggc gcccgcccgg acgcttcgtc
    32341 aggggattac gcgcactctt caacctcgtt ctggttctcg gcgtctccgc tgcggtaggt
    32401 attgcgtgcc tctacctgtt cgtactgagg gagtacgaga gtgacctcga ccggacctac
    32461 cccgagctcg ccgagaactc ctacgtctac gacgtggacg gggacaagat aggcgagatc
    32521 cccgtcaccg agaggcgcga gaccgtcggt ttcgaaggtc tcggcgcgca cctgccaggg
    32581 gccgtcatcg ccgtcgagga ccggcgcttc tacgatcatt tcggtgtcga cttcgaaggg
    32641 ctggccaggg cggcctggac ggatctgaga tcctgggagg tgcaggaggg cggctccacg
    32701 atcaccgagc agctcatgaa gaacctttat gttccagagg gccaacgctt cgaaacctct
    32761 tttcgcaggc gctttgtgca gtctgcgctc gccttcgcct acgagcgcaa acacaccaag
    32821 aacgaggtcc tgaccgccta cctgaatacc gtgtacttcg gggacggcgc atatggcgcc
    32881 gaggaggcgg cgcaacgctt cttcggtaag agcgcgcggg acctcgacct ctcggagtcg
    32941 gcgatgctcg ccggtctgct acacgcccct tcgacctacg ttacctggga gggtgaggtt
    33001 atcgaggggc aggtccgaga gcgccgcgac agcgttctgg ggcttatgca ggaacaaggt
    33061 atgatctcct cccggcagcg cgacgaggcc gaggaccaac cgctggagtt cgcccctgac
    33121 ccgccgccgg aagatccggc gtacacgcct ttcctggaga aggtcgcacg cgaggtggag
    33181 gaggagcttg gtccgcgggc cctgcagctc gggggactga ggatttacac tactatggac
    33241 cccgacctcc aacaggcggc agtggagacc tcggatgatg tgctctcgga accagatgac
    33301 ccttcggcgg ccgtcgtggc aatagagcct cagagcggcg ccatacggtc tctggccgga
    33361 caggatgggg acttcaacct cccgctcgac gccaggcgcc aacccggcag ctcgttcaag
    33421 cccgtagtac tcgcagctgc catcaaggaa gacatctccc cggagagcac ctacatatcc
    33481 caggatctcg ccttcgactt ccgggacgag tactacgagg tacacaacta cgactacgtc
    33541 gagcgcggag agatctccat ctccgacgct atggaggaat cggataatac ggtgttcgtg
    33601 cagcttgctg ccgacgtggg attggaagac gtcgtgaaga ccgccgaaga cctgggcatt
    33661 accaccccgg tcgagcccta cccctcgacg gccataggcg gcctggggtc gggagtcagc
    33721 ccgctcgaca tggcctctgc ctactcgacc ttcgccggtg gcggcatcca ccgcgagcct
    33781 tactccatcg agaggatcga cagaaatagc tacggtcaga gcgagacggt ctacgaccac
    33841 cagatcagcg gccggcgcgt gctaacgggc aacgaagcgg cggtcgcaac ccaggtcctc
    33901 aggggcgttg tggaggacgg gacggcgacg atgttccacg acctggacga ggagatagga
    33961 cgtccatcgg cgggcaagac ggggaccacg gacgacttcg tcgacgcctg gtacgtcggc
    34021 tatactccac ttctttgcac ctcagtgtgg gtcggttatg cggaaggcgg gcgctccctc
    34081 gtcggcgtcc acgacttaaa ggagccgaac ggagagaccc tgcccatgga catctggtcc
    34141 gattacatgg ccagtgctac ggccggggac ctggcgctcg agttccccga agccgacacg
    34201 gccgagctct acgccgtcga gggcggctat gcgggcggct tctgagtcta tggacttgca
    34261 gaggctgtat gccggatctt tcatagattg ggaggcggtc gccgcctcct ggaacaaacg
    34321 aaccgtcccc tcgcggctac tgctcttcgc cgcccgtcgc tacctcgacg aagggggcgg
    34381 taaagtggaa tccgggcgtt atgaagctct gtcatcggct ccgctccccg aggagatcaa
    34441 acgtgccttc gcctcgccgc cggaactcga cgcgccagcc ccgcgccgct ggggcgagtt
    34501 cgtcgacgcc gccgtcgccg ccgagctgga gatggtctcc tacggtgaga gaccgccgct
    34561 cttgcacgag ctgcgggccg ggctcgaaca ggccgcagcg gaggcagacc cactcaccga
    34621 gaagggcaga tggttcctcg ccaggcgcga cgccctcccc gggaccgacc tgcccgacga
    34681 tcccggatat ttgcccatat aagctagtca gcacgctcag ggttcgcctt cgcttgtcag
    34741 ctagtcagct cgccgcttcg cggctctcag ctatgcggcc aggacttgcg caagagaaag
    34801 ctgacgtgct gaagtgctga caagcgcccg acagggcgcg tgctgaaatg ctgacaaggc
    34861 gcgaagcgcc gggctgacat acaatcattc tcgtgccacg cattgggcgg ggcgggtcaa
    34921 ctagggagag gtgttacaca gatggaagct atacccagtg cgagctatag cctcacctta
    34981 cgggtcgaat tcccgcacga ggcgggggcc ctcgggaaga tcctgacgac tattggggat
    35041 gccgacggaa tgatcggcgc cgtcgacata gtgcggatga gccaggacag gaccacccgc
    35101 gacattacag taaacgcccg tgactccgag catggccagc aggtggttca ggtcgtggag
    35161 gacctgcccc aggtcagggt catcaacgtc tcggacagga cgttcttgat gcaccttggg
    35221 ggtaagatcg aggtccgctc caagctccag atccgcaccc gtgatgacct ctctatggtc
    35281 tacacgccgg gtgtggcgcg cgtctgcagg gcgatagccg acgacccgga gagggctttc
    35341 aacctcaccg tcaaacgcaa tacggtagcc gtcgtctctg acggtactgc cgtactcggg
    35401 ctcggggaca taggaccgtg ggccgccatg cctgtgatgg agggcaaggc ggcgctcttc
    35461 aagcagttcg cgaacgtgga cgcgttcccg atctgcctcg acacgaagga tacggacgcg
    35521 atagtcgaga ccgtgaaaaa cctgtcgccc gcgttcggcg gcataaacct ggaggatatc
    35581 tcggccccgc gctgcttcga ggtcgaagag cggctgaaga aggacctgga catcccggtc
    35641 ttccacgacg accagcacgg cacggccgtc gtggttttgg cagccctgat caactccctc
    35701 aagatcgtcg gcaagaaggt ggaggacctt agcatagtgg tgaacggcgt gggcgcctct
    35761 ggggtggcct gcgccaagat catcatggcg gctggcgcga agaacatcgt gggctgcgac
    35821 tcgaagggca tcgtccacga agggcgcgag gacctcaacg agtccaagcg ctggttcgcc
    35881 gagaacacga atccagaggg taggaccggc gacctctccg acgccatagc aggcaccgac
    35941 ctcttcctcg gcctctccgc tccggacgtt ctcgacctcc agcaagtcgg gagcatgaac
    36001 accgacccga tcctgttcgc catggccaac cccgaccccg agataaggcc ggaggtcgcg
    36061 atggggaagg cgaggatcat cgcgacgggc cgcagcgact acccgaacca gatcaacaac
    36121 gtgctctgct tcccgggtat cttcaggggc gccctcgatg tcagggcccg tgagatagac
    36181 gagccgatga agctcgcagc cgccgaggcg atcgcgaacg tcatcccaca agaggacctc
    36241 tccgaggact acatcatccc ttcggtcttc gacgagcggg tcgcccccgc cgtcgcggag
    36301 gcggtcgcgg aaagggccaa ggagacgggc atggccaggc gcgtccagga gcgggccgag
    36361 accggcctga cggtgaccag cttctaaagc gaatcagggt gggtcatcga cccaccctga
    36421 ttcgcgcggt cagcgatcag ccatcagcgg tcagcttccg gcaaaagcag taggctgaaa
    36481 gctgacggct tacaacgtct ggacttctcc ttactgcggc atagaatctg ggcatgagca
    36541 agctgtgcca gatcttcgtt tgcgccacgc cgggcgaggg acgctgcggc gcgaaaggcg
    36601 gcggcgagct gttcgagatg tttagagaag aggtggagct gcgcggcata ccgccatcag
    36661 ccgtgctccg taacgcctgc acccgccgcc acgaggaagg tcccgtcgtc ttcgtctacc
    36721 ccgacgacgt ctggtatacc cgcgtaacac ccgaggacgt gcccgaaata gtggaacgtc
    36781 acctggcgcc tttacgagtc gagagctagt cagcgcgctc cggactttgg gtgccgaagc
    36841 cgacccttcc cagggttcgc tccatcgccc agtctgcgcc ggtgagtatc ctgtcgcgcc
    36901 ggctcttcat gtagtagaga agaacgctcc tgtagagtgc ctgtgccgcg gggcctcgca
    36961 gcctgagacc gacggcgtct gccacggcgc tctccgggcc taggctcacc acgtagcccc
    37021 ggtcgaagaa gcggaaaggc ggcctgcccc gcttgctccc tgcgccgcgg aggcggcgct
    37081 tgaggtcccg cgccacccac ggtccctgtt gcacggcgac ggaggcggtg gggggcaggg
    37141 gaccgaggcg cggatcggtg tagaccccgg cgtcaccgag cacgtagacc tcggggtgac
    37201 caggcaaagt gaggtgctcg cccacgtgtg agcggcccgc cgcatcgtgc tcccccggca
    37261 atcctctcac cagtggcgat gcctccacgc cggccgtcca aaccctggtg cccgccttca
    37321 gccagtcttc gccggcgaag acaccctcgg cgctcgccgt ctctacgggg gtgttcaggc
    37381 gcacttcgac cccgagtccc gtgagcgtct tcctcgcgac gctgtcgaag taggggtcta
    37441 gccaatccat cagccggtgc ttagcctcca gaagcacgac ccgcggttct tcggacggcc
    37501 tgcgatggga gcgtttcccg agatagtcga agagggcggc cacctcggcg gcgacctcga
    37561 cgcccgtcgc cccaccaccg acgatggcaa cggtcagggt gccctgtggc ggccggggcc
    37621 cagggctcgt ggccaggcgc caggcaccgc ccagggcggt gcgcaggcgc agggcatcgt
    37681 ccaggctcca gaaggagtgg aagtagcccg ccagccgcgg tccgggctcg gcggcgacgc
    37741 tgccagggtt gaggaccaga tactcgtagc cgacggggcc ttcagaagtc tcgagggtct
    37801 tcctggcgag gtctataccc gagacttccg cgtgcaggaa cacgcagtcc ccggcgcaca
    37861 agggcgcgat aggtgaggtt acggtcctcg gatgcacccg gccgaccgcg acctcgtgga
    37921 tcaggggcac gaaagcgtac tcttccctgc ggtcgatcag cagggtctcg cctggttttc
    37981 gcagggccgg agggaggtgc ctgagcaggg aggctcccgc gaatccggcg ccgaggacga
    38041 ccacgcgcgc gctgcgccgg atgctcacgc ttcgttccgg cacgtccctg attccttcgc
    38101 cgcatccgtc atccttcggg agtatatcgt tcggggcgcc ccctctcggc gagacgcttt
    38161 acaaaacgcg tcaccgaacg tagactgcac ggatcgcctg ctgatggggt gccggcggtt
    38221 taaggggtgt caggaaaacg gggaacgacc cctccggggg ctagaaaggg tccgtcttgg
    38281 tgatgaagga gggaatggga ctggggaaca tcgcccccgg gctcatcgta aggcacacca
    38341 accgtcgtct gggcaagctg gattcctacg aagcgtgtga gcgtttgatc tctggccgca
    38401 tctcgttctc ggagttcgtg gatcaggtcg gcgaagagta cagggagctt ctcgatttca
    38461 tgatggacaa cgatctgcat cctggtcgtc ccgaggaggt actcttctgc atcgactgtc
    38521 acgcgggtct ctacgaccgt actctccttc gctcgcaact ggaaggcctc tacagtcacg
    38581 acaggagcga aagcatcggc tgacctggga taggcagcgt gccaggccac ctgaggcctg
    38641 gctctgcggc acccattcgt ggttcgtgag gtcgatgtag gaagagcgcc gaccacgccg
    38701 gggcaggacg agtctggcgc gtcccgctgc tatcctgttg gcgtgttttt gcgttatcat
    38761 ggcctccatg ggaaccacct tgataatctt gttcgtcgtg gttgcttcga tcctcgccgc
    38821 ctcaatcgtg ctcgggcgtg acaccaggga ggtagagagc gcccgtgaca cggagtacct
    38881 ggagctcgag ggggtctgga ttcgctacaa cgtcatcggc gggggccccc cggtcgtact
    38941 cgtacacggc tggttatcct cgacccgcat ctgggagcaa ctcgcagggc ggctggcgca
    39001 gcgcttcacc gtgtataccc tcgacctccc cggtttcggg gagtcggaca aaccactctc
    39061 cggatacggg atacgaaacg ggagcaggct tctttacgct ttctgcgccc acttcggcct
    39121 gacccacgcc aacgtcatcg gccacgacct cggcggggcc atggccgtaa agctcgcggc
    39181 agatcatccc gacgtcgtcg gacgcctcgt cgtcatctgt gctcctgccg acgaggacca
    39241 gctcgacctt cccaccctgc tgtggctcgc caccgtgccc gtcgtcggtc ccatcgtcta
    39301 ttctctgggg cgcgcggcgc gccccttccg ccgcctctgg atgcgaccct tcgtagccga
    39361 ctccgacgac ctcacggaag agatagtgga cgacgccggc cggtccacgc ccgccgccgt
    39421 ctccaagacc ttgagcatct cgaggcgtga gatctcccgc ggtaggctcg ccaggcaggc
    39481 caggatcata aagattccct ttctcgtcgt cgccggggaa gaggatcaga tagtagaccc
    39541 tcaatcggtc ggggtatggg ccgagggcgt ggagagggcc gagatctgcc ttatagacga
    39601 gtgcggccac atgcctatgg tggagcgtgt ggccgagttc aacgcccaga tcctcgcctt
    39661 cctgacgggc gacgcccgct acctggattt cgttcacccg gtcgcggaga cgaaagaagg
    39721 ggtcgaaccc gcccgcgtcg aaggcggcga ggtggactac gctgcaccag gcccatcgga
    39781 cgatacggtg gatctcagcc cgcgccccac ggaactcgag tccgaacgcg acaacgtgcc
    39841 gagggtggtg cgtaaacacg gaggaaggta tccggccagg aaccggcctc atcagccacc
    39901 ggagacatac cctgacgcag tcgaagagca accggcccct ggcggtcggt ccagacgtga
    39961 gcctcttgac gaggattttc tacccgacct cccggatgac ctattcgact ggcccgagac
    40021 gcggcgcgac ttcgggtccc gcgagcggcg ccaggatggc ccttcggacg gacaggacga
    40081 cgactacccc cctggtcagg aaaaccctcc ccgctcctaa agctgcttct tcagatacag
    40141 ccactcccgg atgcccataa accaggcggc gatcagggat ctctgccgcg agacctccgc
    40201 gtaaccctcg gcctcgtaga gtcgcagggc gccgaggttg ttgcccgtga cgtgcaggcc
    40261 cacggacttc ttgcccgcgt ccctggcgag gtcttccgcg gccgagagca ggcgccgcgc
    40321 catgccctgg cgccggaagg agggggagat cacgagccgt tcgatgtaag cttcggaagt
    40381 gacggaggag tacgtcgggt aggagagcaa tgtcgtcgcc cagaccgccc gcggaaagcc
    40441 gaggttgcgg gtgagggccc tccacagacc gcgggcgagg atgtcttccc tggagacttc
    40501 cgtcctgacg ccgatgctgc cggcgatctc tccatctgcg tacccttcga ccaccaggga
    40561 agtcaccccg ccagaggtgt gctccaggcg gatccagcgc tccatgacct tgagcgagcg
    40621 gtccatccta ccgccgaaga cgtgcctgaa ctggtccagg aatccttcca cgacgagggc
    40681 ggcgatagcc ggatcatcat caggccgggc gaggcgtatg gtgaactgct cggcctccat
    40741 caggatatcc taccagagtc tcgaccctca gccgataagc cgcctgcgca tgatctggac
    40801 cgggatcaag gcgagcgctc cggtaaacac gagcatccag gccacgtcga cgaggacccc
    40861 taaggaaggt cccgtggcga gctccctgca cacgttgaca gcgtggtaga gcggggtaaa
    40921 ccaggcgatc tgcggcaccg gcgcggggaa gtcgtctacg gggaagaaga cgccggagag
    40981 caaaaatagc ggggtcacga ccagggtaaa gtagtaggca tagaggtcta tgcgttctat
    41041 gaggctggtg aacagcgtgc ccatcacgct gaacatgatg ccgattacga gcagcatcag
    41101 gggcaacagc agggcccacc acgacgtgac gagaggctgc ccgaagacga tgccaagcac
    41161 cactatcatc gcgagaaacg cggtcccgta gagcgccgcg cgcgtccccg cccacagata
    41221 ctcaccaccg accacgtcct ccgcgttgac gggcgtggtt attatggcgt cgtagatctt
    41281 gtctatcttg agcttgacga aggtgttgaa agtgctctcg aaggaggcgg cgaacatggc
    41341 gttgctcgcc aggagcccag gggctacgaa ctggacgtag ggctggccgt tgatgcctcc
    41401 ctgctggatg aacgccccga gccctagccc gagcgccgcc agatagagga gcggctcgac
    41461 gaagttgggg aacaaaatag tcttccaata cttccggaag atggtgatgt tgcgctgcca
    41521 caccctgaac gacccgcggg ccgagggtat ccttatcgta cccactattc caccagcctc
    41581 ctccccgtga gccgcaggaa gacgtcctcc agtccggcct ggcggtacac ggtgttctcg
    41641 atctccgtcc ccgagtcccg cacccgctcg aggagggcgt cggcgtcgtc ggtgtaggcg
    41701 aggatcgact catcgccggt ccgctcgacg gcgtccgaga cggcctcgag aacgggaacg
    41761 agatcctcta gcgacctgtg gtccgtcctg aactcgagga cctgcgggct gacgtgctct
    41821 tcgaccagcg cccccggctt tccttccgcg atgatcaaac ccccctccat gatgaccagc
    41881 ctgtcacaca gccgcgcggc ctcttccatg taatgggtgg tcaggatgag ggtcttgccc
    41941 tcctcggtga gctcgcgcaa ccggtcccag acgagcaggc gggcctgcgg gtcgagcccc
    42001 gtagtcggct cgtcgaggac gacgatctcg ggatcgttca gcagggcgcg ggcgatgagc
    42061 aagcgccgct tcatcccgcc agagagctgc tcgaccgtcg cttcggcctt gtcggagagc
    42121 tgcatgaagt cgagaagctc ctcagccctc ctgcgtacca gcttgctggg aaggtcgtag
    42181 tacctgccgt agatgagcag gttctcctct actttgagct cgtcgtcgag gttgttctcc
    42241 tgggggacga ccccgatgcg gcgtttgatc tcccgctcgt ggtggtagac gtcgagaccg
    42301 gcgacggcga gctctccgcc agtcggtatc acggcaccgt agatcatctt catcgtcgtg
    42361 gtcttcccag ccccgttcgg gcccaaaaaa ccgaaacact ctccaccgaa gacctcgaag
    42421 tcgacgccct tgaccgcctc gaagcccgag aagctcttct tcagaccccg cgcttcgagt
    42481 gtcggctccc cagtcagggc acccttggtt ctctccgtct ccgcgttcaa ccgccaacct
    42541 ctctatgtac ttgggaacgt tcaccagtcc cgaccggcgt ttcggaccac gcaagtgtcg
    42601 gggaagatct cgggaacggc acatatacta ccgtatctcg gctctcggac cagggccaga
    42661 gcagcagcgt cctacggaat ttaaggcccg ccaggctcac cgccatcggc ggagatactt
    42721 tgcctaccat taaggaaagt ttactaacta ctctaccgct ttgtgaaaag tgctgttaaa
    42781 atctcgcgca tgtttcgttc ggcgaagcgg cagcgacgcg gaccgtgtac agcttttgcg
    42841 caggtgggaa gaacggagag ggcacatggg caaaagatct gatcgacgta agttcaggag
    42901 gcgacgcagg tgggccgcgt tcttagtgct tttgtcgctt gccctcatgg tgtttggtgt
    42961 aacgttactg acgggctccc ctgataagca accggttgcc actgatggga tagagagagc
    43021 cgccgtggag agggcggcat ctgtgcaggt ggccggggcc cccgaggctg caagcgaaca
    43081 gaagcgggaa gacgcacagg tcctggcctc agaagaggag gacaagaaga ccacggccga
    43141 gaagaacgag cccgctcaga agccagagcc gaagaagcgg tctacgatac cgacgccgcc
    43201 cacgaatgac ctgtggatgt ccatcccggc gctcgggctc tacgacaact acgtgaccaa
    43261 ctccgcggcc gagtccgcga tggattatgg ggccataaag ttgccccatt cgggcttccc
    43321 ctggcaggac aacgcgaaca cctacattgc ggctcacagg ctagggtggc ccggtacggc
    43381 tagccaccat cagttctaca atctgccgct tctggccaag ggggatgtga tctacctcgg
    43441 cgacgtcaac gggactacct acacttacga ggttacggag ttcaaagaga taacgccgga
    43501 tgaaacatgg gtaacgactc ccaagccggg aagggatatg gtctcgttgc agacgtgcat
    43561 agagaccttc ggtgactact ggacgatggg cccgaactgg tacgtgcgct acatcgtgca
    43621 ggccgacagg gtctccgtgg acgtcgcgga gtaagcgttt cagcccggcg cctctggtgc
    43681 cttgtcagca cgcttcgcct cgctttcagc atttcagctt ttcgctcata gcctctcacg
    43741 cgtagccaaa ggcgcagttc ggagtaggtc gatgcctctg tttatcgttg cgggagcgtc
    43801 gccaggcgcg tttctgtaga gcgtctttga gcgatggtgc actttctttg gtacgacatg
    43861 caaaagcgtc tatacttgcc gctcccgtca cggagacggg tcctggcatt tcgagatagg
    43921 aggataactc acatggcact ggttacgatg cgccagcttc tggacgaggc ggcgaacggg
    43981 ggttacggtg taggggcgtt caacgtcaac aacctggaac aggtacaggc catcatggag
    44041 gccgcgcggg agacagggtc tcccgtcatc atccaggctt cgcgaggcgc acgcaagtac
    44101 acgggcgacc gcttcctgta ccatctcatg gaggccgcga cggagatata ccccgagatc
    44161 tccgtcgccc tgcaccagga ccacggcaac tcccccgaga cctgcaagag cgccatagat
    44221 ctaggcttta ccagcgtcat gatggacggc tcgctcatgg aagacggtaa gacccctgcg
    44281 gacttcgagt acaacgtgaa cgtcacgcgc gaagtggtcg agatggcgca cgcctcaggc
    44341 gtcaccgtag agggcgagct ggggactctc ggcggcatag aggacgacgt tggctccggt
    44401 gaggtgaact tgacggaccc agatcaggcc gtggaattcg tcgagcggac gggggtagac
    44461 gctctggcgg tcgccatcgg gacgagtcac ggggcttaca agttcacgaa agagccggac
    44521 cgcgaggtcc tcgccatgca catcatcgaa gagatcaacg agcggctccc gaccacgcac
    44581 ctcgtcatgc acggctcctc cagcgtaccc aaggaactcg tcgacattat caacgcgtat
    44641 ggcggcgaaa taaagcccac ttggggcgtg cccgtgcggg agatccaaga gggcatcaag
    44701 cacggcgtga ggaagatcaa cgtggacacc gacctgcgtc tggccgcgac gggcgccatc
    44761 cgcaaggcct tcgccgagga cccatcggag ttcgatcccc gctactacct gaagccggcc
    44821 agggcagcga tgcaggaggt cgtaaaggcg cgcatgcagg ccttcggaca ggcgggccac
    44881 gcgacggact acgatcccat ctccctcccg gacatggcag tccgctacaa ggaacaaggt
    44941 caccacctga cggttaccta gagcaaaggt tcttgccagc ccaagagcag aggacggacc
    45001 ctcgtcgagg tcccttaggc ccggtgtctg accaggatgg aacgcgctag tggcgcgccg
    45061 agggcgtggg tgaccccgtc ctcgtcctct gcggtgacga cgccgtccag cgctcggact
    45121 tccctcaccc ggagcgcgcg ccccggcacg agcccaagac cccgcaggta gatgagcacg
    45181 gcagggtccc tgttctctac ccgcgagacg acgaaacgcc cgccgacctc cgtcccgctg
    45241 aggggccgcg aatcctcggg cggcagcgcc ccgtcggacg cggggatggg cgcgccatgg
    45301 ggatcccgct ccggatggcc cagaagctcg gccagacgct ccgtgaactc gtcggagacg
    45361 gcgtgctcca gccgctcggc ctcctcgtgt acgtcctccc acgagtaccc caaatgctcg
    45421 agcaggaacg tctcgatcag acggtgccgc cgcaccagcc gcaaagcctc cgcgcgcccc
    45481 cgcctcgtga gggaagcccc gcggtaaggc tcgtaaaggg ctagtcccat cccctgtaac
    45541 ctcgcgaaca tgttggttac cgaagcggat gagacggaga gcctgtcagc aacgcccttg
    45601 gtcgacgccg ctcccgaacc cgcaatctcc catacggcct tgaggtagtc cccgaccgag
    45661 gcggatggct gagtgaccac tgtgtttccc atctccgatc acaatatagg tgattcgaac
    45721 aattttttca agcaaaagta ctttgacatg taacctcgat ttgtcgtaat attaggcagg
    45781 gctaaaacta actcggggta cgcagtcccg taagggcgca gcacacggaa caggatggcg
    45841 cgcatggata gatcggaatt gggggtttca cgcagggatt tcctgagggc cgcgggcgta
    45901 gccggggctc tgggggtttc gggtagcctg ttgggggccc gcgcggccct cgggcacgac
    45961 ggagaaaagc atggggcgga ccataaggct ggccacgggg ctgctgactc cggcggcatg
    46021 gctgcgcacg gggggaatgg gacggttggg gatgtggacc tctccaggtt cgacccttcg
    46081 aagttcttga gggagttcta ctggggtgag gagcggaccg agggcggcag gacggttcgg
    46141 gagtacgagc tgagtgcgga ggcggtggaa gtagaggtgg cgccgggggt gatgtacccg
    46201 gcgtgggcct acaacgggca ggtacccggg ccaacgttgc gcgccaggga gggtgacttg
    46261 ttgcgggtgg tgttcagaaa cagggacgcg cacccgcaca cgatacactt ccacggcttc
    46321 cacccggcgg gcatggacgg ggtgttcgag cttgtcgcgc cgggccagga gttcgtctac
    46381 gagttcgaag ccgagccgtt cgggtgtcac ctctaccatt gccacgtgat gcccctcaag
    46441 aagcacatcg agaaggggct ctacggagct ttcgtcatag acccgaggga ggggcgtgag
    46501 gaggccgacg agttggtgat ggtcatgaac ggcttcgaca ccaacttcga cggcgcgaac
    46561 gaggtctacg ccgtgaacac cgttgccttc caccaccagc gccatcccgt ggagctgaaa
    46621 ctaggccagc tggtgaggat ctacgtggtc aacgtgctgg agttcgactt cgtcaacagc
    46681 ttccacacgc acgccaactt ctttgactac tatccgacgg ggaccaggct cgagccctcc
    46741 gagttcacgg acaccaagat gttcgcccag ggggagcgcg ggatcatgga gttcagctac
    46801 gacttccccg ggctcttcat gttccacgcc cacgtcagcg agttcgccga gctcggttgg
    46861 atgggcctgt tcgacgtgaa ggagtagggg atgggacgag agcgtggacc ggtctgggtg
    46921 ctcctggggc tcgtgccgct gctggggttg ggcgtgctgc tggccgccat cgtgttgaac
    46981 ggggcgggga tagaccgcga agaccgtcct cccgtggagg acctcaccat cgatcgggtg
    47041 tctttgccca caccggacga gatcgtggtg agcgtgacca atggcggtcc cgagcccgtt
    47101 accgtgagcc aggtgacggt caatgaggcg ctctgggatt tcacggttga gccggacgcc
    47161 aggatctcac cgcttggttc ggcggagatc accctgcctt actcctggat cgagggcgag
    47221 gcatacggga taaccctcat cacggacaca gggaccacct tctccgccga ggtgcctgcg
    47281 gcggtgatca caccgacgtt cacggcggag acgttctggc gctacgcgct gatcgggacc
    47341 tacgtcggcg tgataccggt ggcgctcggg ctgctctggt accccttcct gaggcgcctc
    47401 ggctccgcgg ggataggggc tatacttgcc ctcacgctgg ggctgctggt ctggctggtc
    47461 gtggacacct tgctggaagc ggtggaggta gccgggcggt tgcccggggt cttctccggc
    47521 gtcccgctgg tgctggtggt ggcactgctc gccctgctcg ggctgttggg tgccgagcgt
    47581 ttgttcagga ggcgtgggca gacgagccgg ctctcgacct cttaccggat cgctttgggg
    47641 ataggactgc acaacctggg ggaggggttg gccatcggcg cggcgttcgc cctcggggag
    47701 gcggcactcg ggacgttcct cgtggtcggg ttcacgttgc acaacatcac agaaggcgta
    47761 ggcatcgcgg cgcccatcct cgaagagagg ccaagcctcg cgcacttcgc gggcctggcg
    47821 cttctcggcg gtgggcctgc cgtcctgggg acctggatcg gtggcttcgc ctactccaac
    47881 ctcgcctcgg ccgtgttgct cgccgtcggg gccggcgcca tactacaggt gatctacgag
    47941 gttggccggc ttctcctgaa ggattcggcc cggtcgaaaa cccctgccct ctccgtcccc
    48001 aacctgggcg ggttcaccgc cggtgtggcc ataatgtacg tcacggcatt gctggtgagc
    48061 gtttaggctg actgccgggg cactacaagg gtcgatgtat aggccgccag aaggggccat
    48121 ggtcagaaca ggctgtaccg tgcggcgggc tcaggcaaag ggaagagcgt gaggaagcgt
    48181 tccacagctg cctcgtcgcc ttcgaccctc acgtccccgg atcgcaacgc ttcttccagg
    48241 tcccgatcct ggtagacgag cccggccagc gtagccgaat cggcctcgat gatcgcgtcg
    48301 ggacgctcga cgccgccgcg ttcgacctcg aagccgttcc tggacacctc ggccctgaag
    48361 cggtcctccc ccatcctgag ttcgtagctc gcctccaagc cctccgcagc cccagggtcg
    48421 aacatcgtcc tgaaggagag gatcagggag tcaacaccca gtggagcgtc gccgggtatc
    48481 gacggggagc gggcacccca cctgccgaga cgaataatga ccggctccaa ttcctcgccc
    48541 cattcggtca actcgtagat cttcgacgcc gccggcggag gtagcctcct gcgacgcacg
    48601 atcccggcct cctccaactc gcgcaggcgc tgggcgagta cgttgggact ggcgtgcggc
    48661 aatccagccc gcaagtccgt gaaccgcttc ggccccagca acaactcgcg tactacaagg
    48721 agcgcccagc gctcgcccac aaggtcgagc gcatgcgccg cagcacaccc gtcatcgtat
    48781 gtgcgtcttg tcaccatatg gtgagtttat caccgtaggt cttgaagtct acatatctgg
    48841 tagggtttcg atgatcaatg gttgtttttt aggactactg ccgtattctc agagcagaca
    48901 atgttgcgat ctcgggaaac ggagacggtc cagatgacgc agtatgagtt cggaaaacac
    48961 gaagattcgc atacggagac gatgggtggt aggcggcgtt gggtcgctct gtacgtcctt
    49021 tgtgtgggga tgttgatgat cgtgctcgac gccacggtgg tcaacgtggc gttgccggct
    49081 attcagaacg acctcggctt ctcgcaatcg agccttgcct gggtcgtcaa cgcctacttg
    49141 atctcctttg gcggcctgct gctgctcgcc gggcggcttg gggatttgat ctcccgcagg
    49201 gggatgttct tggccggcat cggtgttttc acggcggcct ccctgttgtg tggtctcgcg
    49261 cagagccagg agttattggt cgcggcccgc tttgttcagg gagccggcgg cgccatgaca
    49321 tcggcggtga ttctgggcat gatcgtcacc atgttccccg agccgcggga gcaggcgaag
    49381 gccatcggcg tcttcgcttt cgtcgcctcc gccggtggtg cggtgggcct gctcgtcggc
    49441 ggcatcctga cccagtccat cagttggcat tggatcttct tcgtgaacgt accgatcggt
    49501 atagtgacgg cggcgctcgc cgggcggctg atcgagggcg acaagggcat cgggttcggt
    49561 gacggcgcag acgtcgccgg tgctgtcctg gtaaccggct cgctgatgct cggcgtctac
    49621 actatcatag gtccggccgc ggagtacggt tggggctccg gacgcaccct gacgatagga
    49681 gccatctcgc tggcgttgtt ggcggccttc gtcgcccgcg aagcgacagc gcgcaacccg
    49741 ctggtgccgc tccggatctt ccgctcgcct aacattacgg gggcgaacct gatccaggtt
    49801 ctgagcgtcg ccgggatgtt cggcatgttc ttcctcggcg cgctctacct gcagcgaatc
    49861 ctcgcctacg acgccctgca gaccggtctt gccttcctgc ccgtaacgat tctcatggga
    49921 acactgtcgg tccgctacac cgaccgcctg gtaatgcgct ttggggccag gaagctcgtt
    49981 ctcggcggtc tcgcgctgtt catgacgggg ctcgcactgt tcgcgcgggc gccggtggac
    50041 ggagattacg tagttgatat attgcccgtg atggtccttc ttggcatcgg agccggtctc
    50101 tgcttcccgg cgctcatgac actggcgatg tctggcgcga cgcccagcga cgcagggctg
    50161 gcctcgggcc tgatcaacac cacagcccag gtcggcggtt ccctcggcct ggccgtgctc
    50221 gcaaccctgt cggcgtcgcg gagtgaccag ctgataggga gcggcgagtc taccgctgcg
    50281 gcgctgacga gtggctacca tctggctttc gggatcggcg ccggactggt cgtcgtggcg
    50341 atagctgtcg cccttactgt gttgcagccc gaacgccaga cagaggaagg gatccagacc
    50401 gaggcggaac ctttcgactg tgaggcggcg taaggtctcg acttgcagct gacctgcaac
    50461 gagaagaggg aggatcatgg acgatatccg gacccagata caggaggcta tcgacgaact
    50521 ggtcgagtcg ggtgcggagc gtggcctgca ggtcaccgtg taccgaaatg gcgagcaggt
    50581 ggtcgacgcg gtcgctggcg tcgctgacca cgagaccgga cgcctggttg aatccggcac
    50641 gcctatctat tgctactcgg tcggcaaggg tgcgacctcg accgtggcgc acgtgctcgt
    50701 cgagcgtggg ttattcggct acgacacccc ggtcgtggac ctttggccgg agttcggcgc
    50761 ccacggtaaa gagactgcga ccgtgcggca cgtgctgacg aatacggtcg gcgtgccagg
    50821 gttaccggac gacaccacgc cggaagacct gtgtgactgg gacaagatgt gcgccgccat
    50881 cgccgatgcc gagccctggt gggagcctgg cacgaagatc gcctatcatg catacccgtt
    50941 cggctatatc gttggggaga tcgtgcgccg ggctacgggc aagccgatat cgcaggtgct
    51001 tcgcgaggag gtggccgaac cgctaggcat cgccgacgag atctacttcg gcgtcccgga
    51061 gtctgagctc ggcaggctgg ccaggctgga ggacatggaa ggcagcgccc agatgcttgc
    51121 ctcgatcccc gacgattctc ctttcttcaa gctcggccca cggcaggtga ccccgaccgc
    51181 agagttcggc aaccgtcccg acatcctgat ggccgacatt ccggccgggg gtaagatgtc
    51241 ggcgcgggcc gtggcgagaa tgtacgccgc gttggtggac gaggtggatg gcgttcggtt
    51301 cgtctcgccc gagcggctgc gcgagatatc cgccgggacg ttcagtggtg tagatgagat
    51361 catgggcttc ccgactgcct gggctctcgg ttactccgtc ggtggccccg gagcagaccc
    51421 gcaagagacg gggagcgtgc tcggggtcgg cggcgttggt gggagcttcg cgtgcgtcga
    51481 caccgcaacg ggcgtcagct gggccctgac gaagaacagg ctcaccgcca gtttcgagtc
    51541 cgcccagcag atctccgaga tcgtcacgaa ggcagtcgaa gaggactagg gctaactcga
    51601 gccgtttgcc ggttagaacg agaggacgtc gacgcagggt tcagcgccag ctctggcgaa
    51661 aggaaggaga gcgacatgga ttggaagctc gaggtcgtga ctatcccggt ctcggacatc
    51721 gatcgggcca gggacttcta ctcggagaag gtcgggttcg tggttgacat cgacttcaag
    51781 gtcagcgacg acgtccacct cgtccagttg actccgcctg gatcggcgtg ttcgatccat
    51841 ctgggaacga gcaccacagc tatggagccg ggctccgtag acggcgtgtt cctggtcgtc
    51901 agtgacgtgc tcgccgcacg cgccgagttg gtggagcggg gcgtggacgt cggtgaagtt
    51961 caggtcttcg aggagggtgc ttaccgcccg gcaagggaag gcgagaatct cgacctcgtg
    52021 gggtgcgtct tcttcagcga tcccgacggt aaccgctggt gcgtgcaaca gatacccgcc
    52081 cgtaactagc ggtggaagga gtgtagacat gggcaaagta acttcggggc tcacgatgtc
    52141 gctcgacggg ttcatcgccg ggccgaacga tgggcctgag catcctctgg gcgagggtgg
    52201 aatgcggctc ttcgactggt actccagcgg cgacaccgat tacgtcgtgc cgaccggcga
    52261 gatgaccttc caggtctcgt cccaggccgc agagatgctg aacgaggtgt tctcctcgat
    52321 aggggccatc gtgacgggac ggaggacgtt cgacatcacg aacgggtggg gcggcagaca
    52381 cccgataggc gaaggtatcc cgatcttcgt cttgacccac tccgtccccg acggatggga
    52441 ctacgagggc tcgccgttca ccttcgtcac cgacggcgta cggagcgcgg tcgaacaggc
    52501 cagggcggcg gccggcgaga agaacgtcgc ggtcggcgct gcgagcctcg tccagcagtg
    52561 cctcgaggcg gggttactgg acgaagtaca cgtagacgtg gtccccgtgc tgttggggga
    52621 cggtgtgagg ttgttcgata acctggggac cgaacccatc gagctggaga ggaccgcgct
    52681 gatagcagac ccggacgtga cccacatgac cttccgggtc ctaaagtaga ggatcgccat
    52741 gagccctcag caagagagca accttccacc cgggttgtcc aggcctgcgc ggcgggcgct
    52801 caccaacgcc ggctaccgga cgctcgagca gctcgccagg gtcagcgaga aggagatcga
    52861 ggatctacat ggcgtggggc caaaggcgct ccagcaactc cggcatacgc tcgttgcgaa
    52921 cggtctgtcg ttcgccgatg ggaacagaac caatcagcaa ggaactcatg aatgaagaga
    52981 caagtgctgt ttgttcacgg aggcggcgaa ggagcatacg aggaagacag gaagatggcg
    53041 gcgagcctgc gagaggcgct gggtggcggc tacgaggtgc attgcccgaa gatgccgcat
    53101 gaggatagcc cgcagtactc ggcgtggaga gaccgggtcg caagtgaact caccgggttc
    53161 gacggcgagg tcttcctcgt cggccattct ctgggggcct cagtcctgct gaagtacatc
    53221 tccgaggaga ggaccgcgac tcccatatcc ggagcgttcc tggttgcgcc accatactgg
    53281 ggcgtcgagg gctgggaggt cgacgagtat gcgctacagg aggacttcgc gtcgaagctc
    53341 cctagtggac taccgatgtt cttctatcac agtcgcgacg acgagtgggt gccgttcgcg
    53401 cacctcgcgc tctatgcgga gaaggttccc catgcaagct tccgcgagtt cgacggacgc
    53461 ggacatcagt tggacgacga tctgtcagag gtcgcccatg atatcgcgag tctggagagg
    53521 accggatcat gactgacgta ccgaaagtgg gatacgcatg aatgaaagct acgcaaatct
    53581 accgcgggta gtgtcgcggg acgagtggaa ggccgcccgc aaggatctgc tcgccagaga
    53641 gaagcgattt actagggaac gcgacgccct gaacgcggat cgccgcaggc tgccgatggt
    53701 caggatcgaa aaggagtacg agttcgaagg acctgacggc aaagcgagcc tgctcgacct
    53761 gttcgagggg cgccgccagc tgattgtcgg ccactttatg ttcgacccgg gctgggagga
    53821 aggatgcccg agttgttcgg cgggggccga tgagatctcg gacgggcttc tgagacacct
    53881 gcacacccgc gacaccacgt tcgtctacgt ctctcgcgtg ccgctggaga agatcgaggc
    53941 ctacaaggcg tccaagggct ggaccttccc ctggtactcc tcccacggca gcgaattcaa
    54001 ctatgacttc cacgcaacac tggacgagtc cgttgcaccc gtcgagtaca acttccagac
    54061 tatagaagac cacgacgagc agtcagccga ggtgccgggc cgaagttctt tcctgcgtca
    54121 gggagagaat atcttccaca cctactccgt ctacgcgcgc ggcctggaga cggttggtgg
    54181 ttcttactac ttcctcgacg agacggccct cgggcggcag gaggactggg aggagcccaa
    54241 aggtcgtgcg gcggacgcgc gtggcgccgt ccccgacttc tcatcgtaga gtttcgaact
    54301 cttgcctgac ctgagctcgg gtcaccctgc cgagaggtgc aggacgtgac ttacagtagt
    54361 ctacgacttc gtgggagaag ctaccggtgt gttgcctccg cgctcagcac aagcatcgaa
    54421 agacctgagc cccggggcct cggctccggg cgtgagatcc agtccaaggt cgatctcgcc
    54481 gtagattgag attcggcgcg aactcgcccg gtctttccac tgtgcgaaat agtgtcttct
    54541 ttgtgtcatg cgcatgcact atgatgtgtg cggtggggta acactggtgg agactcgagt
    54601 tagggacgcg actccaacga ccgctcgccg aagagccacg cgcggcgagg atagaaaggg
    54661 agatcaggga tgcccaggat ggacgttatg gacgcggtgg tgaagttgat ggaggacgag
    54721 ggcgtcgagg tcgccttcgg ggtgcctggc gctgcgatcc tgccactcta ccaggccctg
    54781 ggtaagtccg ggaagatacg tcactattcg gtgcgccacg aagagggcgg gacgcaagcc
    54841 gccgacggct acacccgcgt cacgggcaag gtagggatca acatagggac cagtggtccc
    54901 gccgggacca acatgatcac cggcctctac acctgcatgg ccgactcgat accacagatc
    54961 tgcatcaccg gccaggcccc cacgagcgtg ctacacaagg aagcgttcca ggccgtggac
    55021 atcgtagaga tcgccaagcc tgtgaccaag tgggcggtgc aggtcaaaga gcccgggcag
    55081 ctcgtctgga ccttccggga ggctttccgc atcgcccagg agggccgccg cggcccggtc
    55141 ctgatagacc tcccgctcga cgtacagaaa tcgggcctcg aggtggactt cgacccggag
    55201 cgcggcggtc ccctgaggca catcgccaag ccagagccca gaccaggggc gatccggcgt
    55261 gccatcgaga tgaagcctca gcgggccttc ttggagatga acgagttctt cgacggtgac
    55321 gagatcatcg tcacggcgat aggtctgtac cagatcttct cgggccagtt tcagaaaacc
    55381 tacaagccgc gccattacct ctgctgcggc caggccggcc ggatcgtcgg tggtgtgccc
    55441 gagagagact agggtgcgtc gaagcatgaa agcagaccac acagagatcc tacacgccgg
    55501 cctcgaagcc ggggatccgg cacccatctc gagaccaccc cattatcgga gcccgagacg
    55561 atgaggaaag aagggtcatg aaggacgcgg tgacgaccgt catggagcag tgtgaccgcc
    55621 tcgccgacat cagcgaggag cccggcctta tccaccggcc gtacggttcg aaggccatgc
    55681 acgaggtaaa cgacatcgtt gccggctgga tgcgcacggc cgggatgacc acgagacgtg
    55741 accagatcgg caacctcata ggccgctacg aaggacgcgg cgaaaaaacc ttcatcctcg
    55801 gttcccacct cgacaccgtg cgggatgcag gcaggtacga cggcattctc ggtgtgatgg
    55861 tggcgatcgc gtgcgtccag cggctacacg accggggcga gaggctcccg tattctatag
    55921 aagtagttgg tttcgctgac gaggagggcc tgcggtttgg cacgagctac ctgggaagct
    55981 ccgcctacgc cggctctttc gatcgcgaac gcctgcttct cgaagaccgc aatggtgtaa
    56041 ccctgcggga ggcggtgcgt tccttcggcg gaaacccgga cgccgtcgaa cgtgatggcc
    56101 gtagcgacga gggcctgctc ggctattgcg aggtgcacat agagcagggt ccggtgctgg
    56161 agaaagagga tctgcccgtt ggcgtggtca cgaccatcaa cggccagagt cgtatccagg
    56221 ta
//
LOCUS       DATMVF010000106        29377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_290712, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000106 DATMVF010000000
VERSION     DATMVF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 29377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29377
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290712"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VN178_05020"
     CDS             complement(<1..1055)
                     /locus_tag="VN178_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWS80365.1"
                     /translation="MAKDADLPRSLAGLSGELSARRLSPVEVVSLLLERIEADETNSF
                     ITVTAERALEKASWAEREILAGRYRGPLHGVPVALKDIIYTRGVRTTMGSGPYSGHVP
                     DHSATVARKLEEAGSVLIGKTNTHEFAYGPTGDRSHFGATKNPHDTRRITGGSSGGSG
                     AAVAANLCYGALGSDTGGSIRIPAALCGIVGMKPTFGRVSKSGVFPLSWTLDHVGPIT
                     RCVEDNALMLNVLAGHDPEDPYSEDRPAEDFTRDLQRGLRGASIGLPTSFFFDHVDGE
                     VNARVREAVEVFRSLGARVREVEIPNLCTTLHAHRLILGAEAYAVHQERLESEPETFG
                     EEVGERLMDGERPKAYR"
     gene            complement(1234..2964)
                     /locus_tag="VN178_05025"
     CDS             complement(1234..2964)
                     /locus_tag="VN178_05025"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWS80366.1"
                     /translation="MRRLLDRALAASSNGIVITDPKAPDNPIVYVNPAFEEISGFTAE
                     EVRGRNCRFLQGEHRDQPALDELRKAIAEERECRVVLRNYRKDGTPFWNELYVSPVHD
                     EEGILTNFVSVQNDVTQRRMIEEERDTLLVKEQLARAEAVEARRRLALLAAAGPSLSA
                     SLDYGETLERITRLLVPELADWCLLDIVEDNGSVNQLAAAHAEEEKEDLLRRLREHRE
                     FGEGDPGSTAEVLRTGQSVLLPDLPDSTFYERALGRGEYLDIVLRLEPRSLMCVPLIA
                     RGRTIGAITLVSSRPERIYDGEDLLLAEDLAYRCALAADNARLYRGRSEIARVLQRSL
                     LPPHLPEIPGLEVGAEYLSVGDGSEVGGDFYDLINTVEDGWICAIGDVRGKGVEAASV
                     TALARYTIRAVTLKNDRPSEILAALNEAMLRQLSEDRFCTVACVRLEPQDGPVGVGVD
                     VSRAGHPPPLLVRPEGAVEEVGCSGKVLGVFPEAELNDTSLRLMPGEALVLYTDGVTE
                     ARSPDGDFFGEGRLRRLLSSCAGFDAGTFAGRIKDAVLDFQEGTPSDDLAILVLRARD
                     GAAPAGGEHT"
     gene            3171..3800
                     /locus_tag="VN178_05030"
     CDS             3171..3800
                     /locus_tag="VN178_05030"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWS80367.1"
                     /translation="MAYELPQLPYGYDALEPTIDEQTMHLHHEKHHNTYVTNLNGALE
                     RHPEADPGSLEELLANLDSVPEDIRRAVRNNGGGHSNHSMFWQIMGPSGQGGGEPTGE
                     LASAIDSTFGSFDAFKEEWASMGAPGSVFGSGWVWLIAAPDGSLTIEKTANQDSPLME
                     GKTPVIGLDVWEHAYYLKYQNRRPEYIGAWWEVVNWDEAERRYQAAKNA"
     gene            3970..5346
                     /locus_tag="VN178_05035"
     CDS             3970..5346
                     /locus_tag="VN178_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS80368.1"
                     /translation="MRLVIVGGVAAGTKAASRARRLDPEMEITLYQEEPEPSISECGL
                     PYLLSGVVEERDELVARTPEKFAEKGIEVLVRHRVERIDATGKKLSVLDLNTGETFED
                     TYDRLVIATGARAVLPPLPGAGLEGVFKLRFLTDSDEIARYIQEHSPNRATIVGGGYI
                     GLEVAENLCRLGMEVSLIEGEDRVALAYGPEVSENVEAELEANGVGVYTGEKVDEFTG
                     EGRVTGVKFGDRELQTDLVIVGVGIRPNVELATEAGVEIGTTGAILADRHMQTSVPDV
                     WAAGDCVETTNLVSGRPTWVPLGDTANQMGRVAGTNAATGEETLEFPGILGTGIFKVF
                     DLGVGKTGLSEKEAEDAGFEVVSAGLKANDRAAYYPGATKVFLKLIADRATGRLLGAE
                     SAGSGADKLTDICAAALWGRLSYPDLVNIDLAYAPPYGPALSPVIQAATILSGKFDRA
                     REGVRASE"
     gene            complement(5350..6900)
                     /gene="pruA"
                     /locus_tag="VN178_05040"
     CDS             complement(5350..6900)
                     /gene="pruA"
                     /locus_tag="VN178_05040"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HWS80369.1"
                     /translation="MGLIPYKNEVYLDWSDQKNVEAMRAALREVGSRLDRSYPAIIGG
                     RRVETEGEIVSVNPADPSQVVGRTASATEREADMALDTATRAFETWSRTAPEARARIL
                     LRASAIMKRRKFELLAWEAYEGGKPWFEADAQVAEAIDFLEYYAREMLRLKDGVEIYS
                     VPGEESRYTYQPMGVGVIIAPWNFPTAILTGMSSAAIVAGNTVIMKPSEFTSVIGAVV
                     AEIFEEAGLPEGVLNFLPGYGSEVGDYLVEDARTRFISFTGSMKTGLRINELAARQIE
                     NQRWVKRVIAEMGGKDAMVIDDSADLDSAASDIVKSAYGYSGQKCSAASRAILHADVY
                     DEVLDKVVEKAKALKIGGPESADVNLGPVISEPQFEKVSGYVDVGKQEGDRVLGEDPG
                     DPENGYFISPTIFEVERDARVAREEIFGPVLSVIKAGDFDEALRVANDSPYGLTGGVY
                     SKNRDHLERARREFKAGNVYFNRSITGALVGVQPFGGFGMSGTDSKAGGPDYLPLHML
                     ARTVVERF"
     gene            complement(6910..7818)
                     /locus_tag="VN178_05045"
     CDS             complement(6910..7818)
                     /locus_tag="VN178_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWS80370.1"
                     /translation="MDRAIANSVPVIPRPIVKRISSRYIAGDTLDEAISTIGELNKQG
                     CVATIDLLGESTESKADAAAKLKDYEKVIDALDAHGLDSGISVKLTGLGLTLNEQLCR
                     ANLEEVVEYARERGRFVRVDMEDSPYTEATLRIVEDTHARHGNTGAVLQAYMRRSIED
                     VQLAIRAGYSVRLCKGIYDEPRSIAYKDFDTVRQNYIFLLDELLKGGVYVGIATHDEF
                     LIWHALRLIHQLEVPEDRYEFQMLLGVDEELRSILVGAGHKLRVYVPFGEDWYEYSSR
                     RLKENPKIAGYVARDVIGGITARMKR"
     gene            8004..9563
                     /locus_tag="VN178_05050"
     CDS             8004..9563
                     /locus_tag="VN178_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWS80371.1"
                     /translation="MASIIWGRAPEGAPGGRMTSTNPARTSETVAEVSLGDAGTFVEA
                     CRAARGAQREWASVPAPVRSQVIKKIGRVVENNKEALARLVTREIGKPYAESLGEVQE
                     IIDTCDFFTGEGRRLYGQTVPSEMPDKQLFTFRVPIGVAAIITAGNFPVAVPSWYLIP
                     ALTCGNAVVWKPAEYSPAIGDALARLFMAGGLPDGVLNVVQSEGPAAFEGLEQALDER
                     LVDKVGFTGSSEVGKRIGELCGRHMQSPCLELGGKNPLVVMPDADLDLAVEGALFSGF
                     GTAGQRCTSLGTVIVHESVHDEFLSRFTRVVEESPIGDPTQDVLYGPMLSERFHQRFL
                     GWLELIEPHHSIHGSGGMGRISQDNPREGFVGDAGAGIYDHPTIVDGVTMEDEIYRTE
                     TFGPIVGVASFSDFDEAVALSNGHGYGLSAAIYTTDATNALRFRERNGAGMLSVNNST
                     SGAEAHLPFGGNGKSGNGSRLSGIWVLDQFTRWQSMNWDYAGKLQKAQMDIADLPADL
                     DYTLPEEDKGV"
     gene            9563..10711
                     /gene="solA"
                     /locus_tag="VN178_05055"
     CDS             9563..10711
                     /gene="solA"
                     /locus_tag="VN178_05055"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWS80372.1"
                     /translation="MYDVIVAGLGGMGSSAAYHLSGRGKRVLGLERYSPAHDKGSSHG
                     QSRIIRLAYSEDPAYVPLVLRSYELWERLERETGEDLMTITGGLMIGAPGSKYFEGSK
                     QSAEMYDLPHEVLDAAELKRRYPVFDPTPDTVAFFEKGAGFLRPEASVMAHLDRAASL
                     GADLRFGEGLLSWAPTESGVLVETASGTYEAERLVVSAGAWAPKLLADLGLPLEVTRQ
                     LLFWFDPKGGLEPFLPDRFPIFIWVPEDGNSFYAIPAHDGPGGGVKVAFFRADGKPVD
                     PETIDREVHDEEIEYIRSYLARYVPDLDGDFLHAKTCMYTNTPDEHFVISTHPHYPQV
                     AIAAGFSGHGYKFCSVVGEILADLVTDGETPHPIDLFSPARLVGTARG"
     gene            10797..11177
                     /locus_tag="VN178_05060"
     CDS             10797..11177
                     /locus_tag="VN178_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWS80373.1"
                     /translation="MQNDLIKSQSISGIEVGRDSEEMCLLAKLFNGFANSTRLSILLL
                     LSRRGEMKVGELVDELGAPQPRVSDHLRCLAWCGYVQVRREGRNAFYAVADERVMQIL
                     ELGEELLQDNLEHVEACDDMEKGC"
     gene            11277..12383
                     /locus_tag="VN178_05065"
     CDS             11277..12383
                     /locus_tag="VN178_05065"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80374.1"
                     /translation="MAQDLRQTITSVSGRPGPILSAYLSVNADIPENQRRAYLVRLRD
                     AMNDEGVPEALQQRVREYVEEETHPGARTLAIFAGEDGLFEIYRLQVDVPESFRWGDP
                     YVAPLTLVMDEYEPYGAAVLDAERFRYFVVSPLSTAHGEEEIKANGFREIDVHPSEPY
                     PRGGTDFEPASRRTEAHVHSFYKELGKLTRDVTFREEVQRLILAGPRERTAEFRRTLP
                     NELHDRVVAEVHLEIGAPEGEILDHLEAVRERAEYEREKELLDEIRESGVRGLDETVA
                     ALQEENRVYHLAALWEVEAEIRWCGNDQLAIRDATREDCPFCGQKTRLRPLTDVLVDL
                     AAARGARLDFMRGENENTDTLRDEFGGIAGLTRF"
     gene            12590..13249
                     /locus_tag="VN178_05070"
     CDS             12590..13249
                     /locus_tag="VN178_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HWS80375.1"
                     /translation="MEEYGLVWERFVDGRRLEFGGYMDPAWQTGHRLSASLIVPGEAS
                     RFHERLEPLREALRPFPFVSLHPDHFMHVTLVFLGFLVDEPEEEDEVSRERLQEIEER
                     ARIALSEVPAFTVRFANLNAFPGAAFIEAHDGGALDGLRDALCASCGLKKPQGPPHLT
                     IAYFQAPDGTYAPEELISTIARYRDWPIGEILVDNVEMTLLELRLDYPEPETLARIPL
                     E"
     gene            complement(13373..13879)
                     /locus_tag="VN178_05075"
     CDS             complement(13373..13879)
                     /locus_tag="VN178_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWS80376.1"
                     /translation="MYVYLIRHALAHNRDPERWPDDSKRPLMPKGEETFAEAARGLAL
                     IVPEVEVLLSSPYERAWRTAEILADQAGWPAPETLPALEPDIPPEKVLIALENYAGKK
                     SIALAGHRPGLHELAVYLLTGDAGGADMKIKKGGVACIEFDDAPKAGAGKLRWLFTPK
                     VLRNIAFS"
     gene            complement(13886..15598)
                     /locus_tag="VN178_05080"
     CDS             complement(13886..15598)
                     /locus_tag="VN178_05080"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWS80377.1"
                     /translation="MTTEQVSKSYRTKEPSATDHQELEWQFDAGDLESVESWLGQHDS
                     GSSGLLIAPESTVEITDTYYDTDDWRFYRAGYALRVRKTDGEVEATMKSLTPAEGSLR
                     RRREISEPLSDDKPSTLREAGGAVGGRSRTLVGGRELRPLFTIKTRRQGFALLLEGSA
                     DGDQEDVHIGEISLDTSKIPLGEASARLTRVELEAGIGTAPTPDLLGFVDEMQSVLDL
                     TPASISKYETGLYACGLNPEGDFEFGPTRIDPSMSLGEVAFAVLRTQFAEMRDHEPGT
                     RLGEDPEELHDMRVPTRRMRAAMKVFEGALPERASWLREELRWVAHALGEVRDLDVQI
                     ERFKAWKEAADEESSEYLQKILTITHKRRAEARKNMLETLDSVRYERLLSSFAEMLRL
                     GPAAELELEQANGKDYAGEAVTATAPALVSDRYRKWRKAAKRLDENSSPEAFHDVRKK
                     GKRLRYTLEFFSEVYGKPVQKLVKPLKALQDDLGDHQDAVVTAAYLRELGTKTGEARV
                     PRGVAFTMGVYAERCTREAKDLRSAVPDSKPYRTLTKGKKWKKFGKILESRKAIVIPA
                     KGVR"
     gene            complement(15713..17788)
                     /gene="ppk1"
                     /locus_tag="VN178_05085"
     CDS             complement(15713..17788)
                     /gene="ppk1"
                     /locus_tag="VN178_05085"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HWS80378.1"
                     /translation="MTDVARRPSLSDPSLYINRELSWLGFNNRVLEQARDQRHPLLER
                     VRFVAISETNLDEFFMIRVAGLQQQVASDLPNPVPDGMTPEEQLSRIHDHTEEFFEER
                     RKILNRELIPALEEEGIRTVPHVKVRAAEQRELRERFVRDILPILTPLAIDPAHPFPH
                     ISNLSLNLLVVIEDAGRRVMARVKVPTTIDRFMRLADEDRGPDERPEMRFVRVEEVIS
                     ANLDELFPGKEVVASYVFQVTRNADFVIEEDEAADLLQAIEDELESRWFGQSVRLVVS
                     DEMPDDLRDWLAGNLKVDENSVFASPHPIGLADFEELTHLDRSDLLYPPITPRVPSEI
                     RSARSITQAIRNGDILLYHPYDSFAPVVELVRAAANDPEVLAIKQTLYRVGSNSPIVE
                     TLSEARDEQTQVAVLVELKARFDEEPNITWARQLEARGVHVAYGIVGLKTHAKISLVV
                     RREGSGLRRYVHLGTGNYNPGTARIYTDFSYFTDDPKLVEDGSDLFNYLTGYSEQEEY
                     QELLVAPLTMRESIVGLIEEQIEKARGGEPASITCKTNSLTDPQIIELLYEASQAGVR
                     IDLIVRGICCLRPGVEGVSENIRVVSLVGRFLEHARIFAFSDGDDEKIYLGSADLMQR
                     NLDRRVETSFPLREKRHRERVRRLLDLQLADNANGWDLSPDGTFERLHPKDGEEYVDS
                     QAILLEEGF"
     gene            17950..18396
                     /locus_tag="VN178_05090"
     CDS             17950..18396
                     /locus_tag="VN178_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWS80379.1"
                     /translation="MSIVSARREPASEVEEAISKRGKKKAKKKGLKGGALATLAKPAA
                     KVRDYDAHVLICKDGDCKKRGSKAVRKSLKNELRAEGIHRDVRVDSVGCLGLCKHGPN
                     LVVYPGGTWYLGVVEQEVPEIVQRHLKDGEPVEHLAAELRPRKRAK"
     gene            complement(18469..19050)
                     /locus_tag="VN178_05095"
     CDS             complement(18469..19050)
                     /locus_tag="VN178_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWS80380.1"
                     /translation="MRTYRTRRSGLRIFGYLVATLLVVSGLVLMGYSFLIRDPFASAA
                     VFAQKPPSDSTMTLTIPEMRRVDRVPVYTGAANDKAALHDGTLHVKGTGFPWQTGANV
                     YIAGHRLGFPGTRSYLVFWDLNKLGDGDKVILTDANGTRYTYNVFKRTIINPDEYYVT
                     RPVPGKSVITLQTCTLPDYAQRLIVQAELTAVG"
     gene            complement(19178..19861)
                     /locus_tag="VN178_05100"
     CDS             complement(19178..19861)
                     /locus_tag="VN178_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWS80381.1"
                     /translation="MKKLSTTYEKWRLATLAVAVFAFAGIGLLGYSLLSGGAPASADS
                     PDRVHGRISEELERKFNRSIPERIVETAQDPLVGSRARTPESKQLELTVPDLDRVDGV
                     PVYDAPASGYEEALHDGVVHVRGTGFPWQDVANVYIAGHRVGYPGTRSDLVFWDLDKL
                     EKGDEILLTDADGTRYTYQVFEKRVISPDTVDIMRPTKGKNIVSLQTCTLPDYSHRLI
                     VQGELKGKG"
     gene            20011..21300
                     /locus_tag="VN178_05105"
     CDS             20011..21300
                     /locus_tag="VN178_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HWS80382.1"
                     /translation="MKVGIPKEVAEGERRVGLVPETVGRLVKDGFEVLVESGAGRDYN
                     LDANYEEAGAKIVGGADGVYGEADIVVKVQSPREDELGLLREGQVLICFLNGPQEPEL
                     VEKLAEAGVTVFSNEAVPRTSAAQAMDALSSMGSIAGYKCALIAADTIGKYVPMLSTA
                     AGTTKAANVMVFGVAVAGLQAIAIMHRLGGEVFAYDIRPETKEQAQSLGATFMDSEDE
                     KEEEKEDPFEEYQPTGWRKLMTSLGFYSYAEPPRDQYIVEGEEEEEANSEEEDEGWSQ
                     EKLDEDQKLIRERMEEMDIVITTALVPGRKAPQLVDKDMVESMKPGSVIVDLAAESGG
                     NCELTEVGETVEHEGVRIVGPVNLPSTMPIHASQLYSRNMYNLIGHITEDRSEDKDEK
                     DLHLDLDFEDEITGETCIAHGGEIRHGPTREALQEAG"
     gene            21297..21605
                     /locus_tag="VN178_05110"
     CDS             21297..21605
                     /locus_tag="VN178_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HWS80383.1"
                     /translation="MTSQLLAQLAIFVISAFLGFELISRVPNLLHTPLMSATNAIHGI
                     VLVGAIIALASASDGFTTTVGMIAVIFGAMNVVGGFWVTNRMLGMFGPPRKRVKKREE
                     "
     gene            21609..23021
                     /locus_tag="VN178_05115"
     CDS             21609..23021
                     /locus_tag="VN178_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWS80384.1"
                     /translation="MQVAPFIAYLAAAALFIYGIRRLRTPETARSGNTLAAVGMAIAL
                     IATIFVADIDNVLSVGQISVGVLIGSVLGAVAAQRVQMTAMPQMVAAFNGVGGGAAAL
                     VAVSEFYRVEALGQGHETLVATIAVLLSVIIGTVSFAGSTIAFIKLQELALTGSVTFP
                     LQQVVNAAIFVGILLLSANILAGGLVLSPLVSLYLILGASLLLGVLFVIPIGGADMPI
                     VISLLNAFTGLAAAASGFVLSNYALIISGTLVGASGTILTRLMSGALGRPLGKIIFAG
                     IAASGGGGSEQQEDREVNDKSAGEVGEYLADDAEKVIIVPGYGLAVAQAQQAMRDLVE
                     TLESEGKEVLFGIHPVAGRMPGHMNVLLTEVGIPYEKLYDLEDINPEFDDADAAIIVG
                     ANDVVNPSANDPEVESPISGMPVLTVENAEHVIFIKRSLSPGFAGVDNPLFYDTEKTM
                     MLFSDAKQGLQDLNEAVKNS"
     gene            complement(23502..23645)
                     /locus_tag="VN178_05120"
     CDS             complement(23502..23645)
                     /locus_tag="VN178_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS80385.1"
                     /translation="MSRGLTNRRIASELFLSEHTVHHHVTNTLKKLNLTSRQQVASHL
                     PDR"
     gene            complement(23776..24780)
                     /locus_tag="VN178_05125"
     CDS             complement(23776..24780)
                     /locus_tag="VN178_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS80386.1"
                     /translation="MSGKAGNPHTGIEIGRTLRLAREKRGLSLQQVEEATKIRTRYLR
                     DLENENFDVLPAVYMLGSLKTYAEHLGLDGAAIARELKRRQASLQPEQDQAPEDSPSD
                     EPRGFLASLGRLAGIGGTVEDEAGAMAPVRRPGLYVSLVVVLLFVLATYLASSIRSED
                     LPPVSPVREPTVSQLPSGIVLVGNVEDAERNPEDLSEEKQAEPPSKDGETRAEQSGQE
                     EHAPRSAHAPSSSAIASASASAASASSASPAYTGSASAPATVAPGPTKVRPEPAAGEQ
                     RGGESMVAPADPPARVSRSQSLHETQAGPVGATRPGDGIPNKAKEAVDSVCGPCFAGE
                     "
     gene            complement(24952..26460)
                     /locus_tag="VN178_05130"
     CDS             complement(24952..26460)
                     /locus_tag="VN178_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80387.1"
                     /translation="MMRRVAVAALAFSLILSGARPALAHGFGPTYDIPIPLWLYLYGA
                     AAAVVLSFLPLILFSRKERYSAYDYPRFDLFRVRLLRTLLTSNLLIGGLRLFSVALFL
                     VVLITGLVGLQSGFNFAPTFVWIIWWVGLSLFTAFVGNLWPLVNPWRVVFDWAERLVR
                     RLGYRDGLELGEPYPEALGIWPAVGLYLVFVWIENVFSGSYVPRYIALFALAYSLITL
                     YGMAFFGKEIWLRRGDAFSVFFGLLGRFAPTEVRVKDAEVCLGCDGCGDDARGGCVNC
                     YECFARAAPEERELNLRPPAVGLGLPEKVTPGGAAFVIVVLAGVTFDGLLETPLWLEI
                     VRLTPVTQTLGTILLPLLFLAIYLGFVELSRVFGGGGGIGIRRFAAAYAFSLVPIAIA
                     YQAAHYYTYLLVQGQMIVSLVSDPLGWDWNLFGTADFEPRYGIVGAGFVWYSQVALIV
                     AGHVIAVYLAHSISLRLLRDPGRALKSQLPMLVLMVLYTMTSLWILAQPIVE"
     gene            complement(26468..26824)
                     /locus_tag="VN178_05135"
     CDS             complement(26468..26824)
                     /locus_tag="VN178_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWS80388.1"
                     /translation="MARSMILIAVIIVILGGLFFLLRPDTPAGVPRERTIAVSIEGGE
                     MSPAEISVNEDDSVTLRVSSDRPMELHLHGYDVEGEVGPGQKASLRFEADLTGRFEIE
                     DHGSERELGVLQVRPR"
     gene            complement(26893..27807)
                     /locus_tag="VN178_05140"
     CDS             complement(26893..27807)
                     /locus_tag="VN178_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80389.1"
                     /translation="MKIETIDLEFMDTGQVIASFLLLGEDGASAALVETGPTNCLDRL
                     TAGLKDRGVSHEDVRQVFLTHIHLDHAGASGHLSGMLPNATFYVHEVGYPHHVDPSKL
                     VKSATRIYGERMDELWGEARPVPEDRIRVLKDGVETEAAGGVLVAHDTPGHAYHHLAY
                     LEPDSGALFTGDVAGIRLPGQSYIRPPTPPPEIDVEAWIQSINYMRQIDPACLWLTHF
                     GSYEDVGRHLGELEQRLQDWLLFVEGQMDEGSGREEISDQLRAKGDAEMLAEGSDITE
                     SERYGLAGSYSMLTDGLMRYATRQREKR"
     gene            27901..29109
                     /locus_tag="VN178_05145"
     CDS             27901..29109
                     /locus_tag="VN178_05145"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWS80390.1"
                     /translation="MDLNLMRELSVKTETKIVLLILDGLGGLPMDPSGHTELEAAETP
                     NLDALAARSDLGLSQPVAAGVSPGSGPGHLGLFGYDPVRFQVGRGVLSALGVGFDLGE
                     SDLATRVNFATKDESGRISDRRAGRIPSEKGEELVALLNEKLNLDGVEIYVVPEKEYR
                     AVAVFRGEGLSDALADSDPQRTGLEPLPVDPTDGSPEAKKSAQIANSFVEQANEILAD
                     QHPANTVLVRGFGMHPALPSFEETYRLDAAAIASYPMYKGLARLAGMHLLKEGEGISG
                     EFETLKTNWGDHDFFFVHIKPTDAAGEDGDFDRKASVIEEVDARIPALLDLGPDALAI
                     TGDHATPAKLKSHSWHGVPFLLASPYTLPTADSFGERTCAGGSLGVFQAEEIMGYLMG
                     HALKLNRYGA"
BASE COUNT         5437 a         9226 c         9310 g         5404 t
ORIGIN      
        1 tagcggtacg ctttcggacg ctcgccgtcc atcaggcgct cgccgacctc ttcgccgaac
       61 gtctccggtt cgctttcgag acgttcctgg tggacggcgt aggcctctgc gccaaggatc
      121 aaccggtgcg cgtggagcgt agtgcacagg ttgggaatct cgacctccct tactcgcgcg
      181 ccaagtgacc ggaagacctc cacggcctcg cgcacccgcg cattgacctc cccatcgacg
      241 tgatcgaaga agaagctcgt cggaagaccg atactggctc cccggagtcc gcgttggaga
      301 tcccgcgtaa agtcctcggc gggccggtct tcggagtagg ggtcttccgg gtcgtggccg
      361 gccaggacgt tcagcatcag ggcgttgtcc tccacgcagc gggtgatggg accgacatga
      421 tccaacgtcc acgagagtgg gaagacaccg ctcttgctca cccgcccgaa ggtcggcttc
      481 atgccgacga taccgcagag ggccgcaggg atacggatgg agcctcccgt gtccgaacct
      541 aaagcgccgt agcagaggtt tgccgcgacc gcggcccccg agccgccact ggagccgccc
      601 gttatcctgc gcgtatcgtg gggatttttc gttgccccga agtgcgaacg gtcgcctgtg
      661 ggaccgtagg cgaactcgtg agtgttggtc ttgccgatga ggacggatcc cgcctcttcc
      721 agcttccgcg cgaccgtcgc gctgtggtcc gggacgtgtc cggaataagg gccggagccc
      781 atcgtcgtac ggacacccct ggtgtagatg atgtccttca gggccacagg cacgccgtgc
      841 agcgggcccc tgtagcgtcc ggccaggatc tctcgttcgg cccacgacgc cttctccaga
      901 gcccgctccg ccgtaacggt gatgaaggag ttcgtctcgt ccgcctcgat acgctccagc
      961 aacaagctca cgacctcgac gggagagagg cgtctcgccg aaagctcccc ggagaggccg
     1021 gcgagcgagc gtggcaggtc tgcgtctttg gccaagcctt cgctccttcc agggtagacg
     1081 tgtgcagtat ctcatagcgt tccggtgtgt ttccgggatt atcggccggt gaatacacac
     1141 gcgaggaaat ctctccgtag gttcgatcgc agggtgtggc tcttgctctt cgcgatctgg
     1201 accatctagc tcgcggcatc cgcggcggtg ggattaggtg tgctctcccc ccgcgggcgc
     1261 cgctccgtca cgggcgcgta ggaccaggat tgcgaggtcg tcgctcgggg tgccctcctg
     1321 gaagtcaagg acagcgtcct tgatcctccc ggcgaacgtc ccagcgtcga agccggcgca
     1381 ggagctgagg agacggcgca acctgccttc gccgaagaag tcaccgtcgg gggagcgcgc
     1441 ctcggtgacg ccgtcggtgt agaggacgag cgcctcccct ggcatgagac gcagcgacgt
     1501 atcattgagc tccgcctcgg ggaacacgcc aagcactttg cccgaacagc ctacctcctc
     1561 cacagctcct tcggggcgaa ccaggagcgg tggcggatgg cctgcgcgtg agacgtcgac
     1621 gcctacgccc accgggccgt cttggggctc gagccggacg caggccaccg tgcagaagcg
     1681 atcctcggat agctggcgga gcatagcctc gttgagggcg gcgaggatct cggagggcct
     1741 gtcgttcttg agggttacgg cgcggatggt gtagcgagcc agggccgtta ccgatgccgc
     1801 ctccactccc ttgccgcgca cgtccccgat ggcgcagatc caaccgtcct ccacggtgtt
     1861 tatgaggtcg tagaagtctc ctcccacctc gctgccatcc ccgacggaca ggtactcggc
     1921 accgacctcg aggccaggga tctcgggtag gtgtggcgga agcaggctcc tctgcaagac
     1981 gcgcgcgatc tcgcttcgac ccctgtagag gcgggcgttg tctgcggcca gcgcgcagcg
     2041 gtaagccaga tcctcagcga gcaggaggtc ctcaccgtcg tagatgcgct ccggcctcga
     2101 tgacacgagg gtgatagcgc ctatggtcct ccctcgcgca atgagcggca cgcacatcaa
     2161 cgagcgaggc tccaggcgga gtacgatgtc gaggtactcg ccacggccca gcgcccgctc
     2221 gtagaaggtg gagtcgggca gatcggggag cagcacagac tgcccggttc tgagcacctc
     2281 ggccgtgctc cccgggtccc cttccccgaa ctccctgtgc tccctgagcc tcctcaggag
     2341 gtcctccttc tcttcttcgg cgtgcgcggc ggcgagctgg ttcacgcttc cgttatcttc
     2401 cactatgtcg agcagacacc aatccgcgag ctcgggcacg aggaggcggg tgatccgctc
     2461 caaagtctct ccgtagtcga gcgaggcgga gagcgacgga cccgccgccg ccagcaacgc
     2521 cagtcgccgc ctcgcctcca ccgcctcggc ccgcgcgagc tgctccttca cgagcagcgt
     2581 gtcacgctcc tcctcgatca tgcgccgttg tgtaacgtcg ttctggacgc tgacgaagtt
     2641 ggtcagaatc ccttcttcgt cgtggacggg cgagacgtac agctcgttcc agaacggcgt
     2701 gccatccttg cggtagttcc tcaggactac cctgcactcc cgctcttccg cgatcgcctt
     2761 gcgcaactcg tcgagtgcgg gctggtcgcg atgctcgccc tgcaaaaagc ggcagttccg
     2821 tcccctgacc tcctccgcgg tgaagcccga gatctcctcg aaggcggggt ttacgtagac
     2881 gatgggattg tcgggcgcct tcgggtccgt aatgacgatg ccgttcgagc tcgcggcgag
     2941 cgcccgatcg agcagacgcc gcatctcctc cgaagcgggc cgctccgggt catcaccgcg
     3001 ccgggtacgt gaagaggaag tcgccaatcc tcgtatttta cagctttcca cgacctcaca
     3061 tccaccgtgt gaggatgacc gcgagaactt ctcccgacgt taagagcttg tgacttcggg
     3121 gtataactcg ctgggtacag aaaagccgga gtacgaaagg agaaacgaaa gtggcttacg
     3181 agctaccaca actgccgtac ggttacgatg ccttggagcc cacgatagac gaacagacca
     3241 tgcacctcca ccacgaaaaa caccacaata cctatgtgac caacctcaat ggtgccctgg
     3301 agaggcaccc cgaggctgac ccgggctccc tggaagaact cctcgccaac ctggattccg
     3361 tcccggagga catcaggaga gccgtaagga acaacggtgg ggggcactcc aaccactcca
     3421 tgttctggca gataatggga ccctccggcc agggaggagg agagcccacg ggagagctcg
     3481 catctgccat agactccacg ttcggatcgt tcgacgcctt caaggaggag tgggccagca
     3541 tgggggctcc aggcagcgtg ttcggatcgg ggtgggtgtg gctcatcgcc gcccccgacg
     3601 ggtcgctcac catcgagaag acggccaacc aggactcgcc cttgatggaa ggcaagacgc
     3661 ctgtgatagg tctagacgtg tgggagcacg cctactacct gaagtaccag aaccgccgac
     3721 ccgagtacat cggagcctgg tgggaggtcg taaactggga cgaggccgag agacgctacc
     3781 aggccgcaaa gaacgcctaa cccaaagttc tctatagtac gcacgtgaga gcggaagacc
     3841 cctcgagtgc actccagggg tcttcttctt gcgccggatt gcgcgtccgg acgttgtaat
     3901 agagactcct tcgcagtcgg ggcaaagcct gtagactagg gtggtaggca cagtgggagg
     3961 atctcatcca tgcggcttgt gattgtgggc ggcgttgcgg ctggaaccaa ggccgcctcg
     4021 agggcgcgca ggctcgaccc cgagatggag attacgctct accaggaaga accggagccc
     4081 tccataagcg agtgcggcct gccctacctc ctctccggcg tcgtcgaaga acgagacgag
     4141 ctcgtggcgc gcacgcccga gaagttcgcg gagaaaggca tcgaggttct ggtccgtcac
     4201 agggtggaga ggatagacgc taccggcaag aagctttcgg tactggacct taatacgggc
     4261 gagaccttcg aagacaccta cgaccggctc gtcatcgcga ccggcgcgcg ggccgtcctc
     4321 ccccccttgc ccggcgcggg gctggaaggc gtcttcaagc ttcgtttctt gacggactcc
     4381 gatgagatag ccagatacat tcaggagcac tctcccaaca gagcaacgat agtcggaggg
     4441 ggctacatcg ggctagaggt cgccgagaac ctgtgccggc tcgggatgga ggtctccctg
     4501 atcgagggcg aagacagggt cgccctcgcc tacggacccg aggtctcgga gaacgtggag
     4561 gccgagctcg aagccaacgg cgtaggcgtc tacacgggcg agaaggtcga tgagttcacc
     4621 ggcgaaggac gtgtcacggg cgtgaagttc ggggacaggg agctccagac ggacctggtc
     4681 atcgtcggcg tcgggataag gccgaacgtc gagcttgcca cggaagcagg ggtcgagata
     4741 ggaaccacgg gagccatcct ggcggaccgt cacatgcaga ccagcgtccc cgacgtctgg
     4801 gccgccggag attgtgtgga gacgaccaac ctggtgagcg gcaggcctac ctgggtccct
     4861 ctcggcgata ccgcaaacca gatgggccgc gttgcgggca cgaacgcggc cacgggtgaa
     4921 gagaccctgg agttccccgg catactaggc acgggcatct tcaaggtctt cgacctcggc
     4981 gtgggcaaga cgggactctc cgagaaagag gccgaggacg ccggcttcga agtagtgagc
     5041 gccggactga aggccaacga tcgcgcggcc tactaccccg gcgccactaa agtcttcctc
     5101 aagcttatag cagaccgggc gacgggcagg ctgctcggcg cggagtctgc gggctctggc
     5161 gccgacaaac taaccgacat ctgcgcagcg gcgctctggg gcagactctc ctaccctgac
     5221 ctcgtcaaca tagacctggc ctacgccccg ccgtacggtc ccgccctgag ccccgtcata
     5281 caggcggcca ccatcctctc gggcaagttc gaccgtgcca gggaaggcgt cagggcctcg
     5341 gagtaggcgt cagaacctct cgaccactgt tctggcgagc atgtggagcg gcaggtagtc
     5401 ggggccgccg gccttggagt ctgtgccgct cataccgaag cccccgaagg gctggacgcc
     5461 gacgagggcg ccggtgatgc tgcggttgaa gtagacgttg ccggccttga actcccggcg
     5521 cgcccgctcg aggtggtcgc ggtttttgga gtagacgccg cccgtgaggc cgtaagggct
     5581 gtcgttggcg acgcggaggg cttcgtcgaa gtcgccggcc tttatgaccg agaggacggg
     5641 accgaagatc tcctcgcgcg caaccctggc atcccgctct acctcgaaga tggtcgggct
     5701 gatgaagtag ccgttttcgg gatctcccgg gtcttcgccg agcacgcgat cgccctcctg
     5761 cttgcccacg tccacgtaac ccgacacctt ctcgaactgc ggctcgctta tgacgggtcc
     5821 caggttcacg tcggcgctct cggggccgcc gatcttgagc gccttcgcct tctcgacgac
     5881 cttatccagg acctcgtcgt agacgtcggc gtgcaatatg gcgcggctcg ccgcgctgca
     5941 cttctggccc gagtagccgt aggcgctctt gacgatgtcc gaggcggcgg agtcgaggtc
     6001 cgccgagtcg tctatcacca tcgcgtcctt gccgcccatc tcggctatga ccctcttgac
     6061 ccagcgctgg ttctctatct gcctggccgc caactcgttg atgcgcagac cagtcttcat
     6121 cgagccggta aaggagatga aacgggtcct cgcgtcctct accaggtaat ccccgacctc
     6181 cgagccgtag cccggtagga agttaaggac gccctccggc aggccggcct cctcgaagat
     6241 ctcggccacc acggcgccga tgacgctcgt gaactccgag ggcttcatga taacggtgtt
     6301 ccccgcgacg atggcggcgc tagacatccc cgtgaggatg gccgtaggga agttccaggg
     6361 ggcgatgatg accccgaccc ccatcggctg gtaggtgtag cggctctcct cgccggggac
     6421 ggagtagatc tcgaccccgt ccttgaggcg cagcatctct cgggcgtaat actccaggaa
     6481 atctatcgcc tcggcgacct gggcgtcggc ctcgaaccag ggcttgccgc cctcgtaggc
     6541 ctcccaggcg agcagctcga acttgcgccg cttcatgatc gccgacgccc tgagcaggat
     6601 gcgcgccctt gcctccggcg ccgtacggct ccaggtctcg aaggctctgg tggccgtgtc
     6661 gagcgccatg tccgcctcgc gctcggttgc gctggcggtg cggcccacca cttgcgaagg
     6721 atccgccgga ttcaccgaca ctatctcgcc ttccgtctcg acgcgcctgc cgccgataat
     6781 cgcggggtag cttcggtcca gcctggagcc cacctctctg agggcggccc gcatggcttc
     6841 gacgttcttc tgatcggacc agtcgagata cacttcgttc ttgtacggga tcagtcccat
     6901 gtcaggctcc tatctcttca tcctggctgt tatgcccccg atgacgtccc tggcgacgta
     6961 gccggcgatc ttggggttct ccttgagacg cctactcgag tactcgtacc agtcctctcc
     7021 gaagggcacg tagaccctga gcttgtgccc ggccccgacg aggatgctcc tcagctcctc
     7081 gtctacccca aggagcatct ggaactcgta gcggtcctcg gggacttcga gctgatggat
     7141 gagccttagg gcgtgccaga tcaggaactc gtcgtgcgtc gctatgccga cgtaaacgcc
     7201 acccttcaag agttcgtcga gcaggaatat gtagttctgt cggacggtat cgaagtcctt
     7261 gtaggctatg gacctcggct cgtcgtagat gcccttgcac aaacgcacgg agtaaccggc
     7321 tcgtatggcg agctgcacgt cctcgatgct gcggcgcatg taggcctgca ggaccgctcc
     7381 cgtgttcccg tgcctggcgt gtgtatcctc gacgatccgc agcgtcgcct ccgtgtaagg
     7441 cgagtcttcc atgtcgaccc gcacgaaacg accccgttcc cttgcgtact cgacgacttc
     7501 ctccaggttc gcccggcaga gctgctcgtt cagggtcagt ccgagcccgg tgagcttgac
     7561 cgagatgccg ctgtcgaggc cgtgcgcgtc gagcgcgtcg atgaccttct cgtagtcttt
     7621 cagcttcgcg gcggcgtcgg ccttgctctc ggtgctctcg ccgagtaggt cgatggtggc
     7681 gacgcaaccc tgcttgttga gctctccgat cgtggagatc gcctcatcca gcgtatcgcc
     7741 ggctatgtag cggctggaga tccttttcac gatgggccgt gggatgaccg gcaccgagtt
     7801 cgctatggct ctgtccacta ttcccaagtc tcgaaacccc gcaagacgga ctgtgtgacg
     7861 ctctatgcgc cctgatttta ctgtaaggag cgtcccgagt ctttggtgta ggatagtgac
     7921 aagataggcg agcgagagag gcctggaggt agagaatgcg taccaccgac gcgcgtgaag
     7981 gaacagcttc agagacccgt agggtggcct cgataatatg ggggcgcgct cccgagggcg
     8041 cgcccggcgg gcgcatgact tcgacgaacc cggcgcgcac gtcggagacg gtggccgaag
     8101 tgtcgctcgg ggacgcaggg acgttcgtag aggcgtgccg ggccgcgcgc ggggcgcagc
     8161 gggagtgggc atctgtgccc gcccctgtcc gctctcaggt gataaagaag atcggtcgcg
     8221 tagtcgagaa caacaaggag gcgctagcca ggctggtgac ccgggagatc ggcaagccct
     8281 acgctgagtc tctcggagag gtgcaggaga tcatagacac ctgcgacttc ttcaccggtg
     8341 agggtcgcag gctctacggt cagaccgtac cctcggagat gccggacaaa caactcttca
     8401 ccttccgcgt tcctatcggc gtcgcggcca tcatcacggc cggcaacttc cccgtggcgg
     8461 tcccttcgtg gtacctgatc cccgccctca cctgcggcaa cgccgtggtc tggaagccgg
     8521 ccgaatactc ccccgctatc ggcgacgccc tggccaggct attcatggcc ggaggccttc
     8581 ccgacggcgt cctgaacgtg gtgcagtccg agggcccggc ggccttcgaa gggttggagc
     8641 aggccctcga cgaaaggctc gtcgacaagg tcggctttac gggctcctcc gaggtcggga
     8701 agaggatagg cgagctctgc ggcaggcaca tgcagtctcc ctgcctggag ctcgggggca
     8761 agaatcccct cgtggtcatg cccgacgcgg acctcgatct cgccgtcgaa ggcgccctgt
     8821 tctcgggctt cggcacggcg ggccagcgct gcacctcttt gggcaccgtg atcgtccacg
     8881 agtccgtcca cgacgagttc ctctccaggt tcacccgcgt cgtcgaggag tcgcccatag
     8941 gagacccgac gcaggacgtt ctctacgggc ccatgctaag cgagcgcttc caccagcggt
     9001 ttttgggctg gctcgagctg atcgagcccc atcacagcat ccacggttcg ggcgggatgg
     9061 gcaggatctc ccaggataac cctcgcgagg gcttcgtcgg cgacgccggg gccggcatct
     9121 acgaccaccc gaccatcgtc gacggcgtta cgatggagga tgagatctac cgcacagaga
     9181 ccttcggccc catcgtcggc gtcgcctctt tctcggactt cgacgaggcc gtagccctct
     9241 ctaacgggca cggttacggg ctctcggccg ccatctacac caccgacgcg acgaacgctc
     9301 tgcgcttcag ggaacgcaac ggcgcgggga tgctctccgt caacaactcc accagcggcg
     9361 cggaggccca cctgcccttc gggggcaacg gcaagagcgg gaacgggagc cgcctctcgg
     9421 gcatctgggt actcgaccag ttcacccgct ggcagtccat gaactgggac tacgccggca
     9481 agctccagaa ggcccagatg gacatagccg acctccccgc cgacctcgac tacaccctgc
     9541 ccgaggaaga caaaggcgtc tgatgtacga cgtgatcgtc gccggcctcg gtgggatggg
     9601 gagctcggcg gcctaccacc tgtccgggcg tggcaagcgc gtgctcgggc tggagcggta
     9661 ctcaccggcg cacgacaaag gctccagcca cgggcaatcc aggatcatcc gcctggcgta
     9721 ctccgaagat cccgcctacg tacctctggt cctgaggtcc tacgagctgt gggagcggct
     9781 ggagagggag acgggcgagg atctgatgac gatcacgggc gggctcatga tcggggctcc
     9841 gggcagcaag tacttcgagg gcagcaagca gagcgccgag atgtacgatc tgccccacga
     9901 ggttctcgac gccgccgaac tgaagaggcg ctacccggtc ttcgacccaa cccccgatac
     9961 cgtcgccttc ttcgagaagg gggcaggctt cctcaggccc gaggcttccg tgatggccca
    10021 cctggacagg gccgcctccc tgggcgctga cctgcgcttc ggggagggac tgctgtcctg
    10081 ggcacctacc gaatcagggg tactcgtgga gacggcatcg gggacttacg aggcggagcg
    10141 gttggtagtc tcggccgggg cgtgggctcc gaagctgctc gctgacctgg gcctcccgct
    10201 agaggttacg cgccagctcc tgttctggtt cgatcccaaa ggcggcctcg aacccttcct
    10261 cccggaccgc ttccccatct tcatctgggt acccgaggac ggcaactcgt tctacgccat
    10321 acccgcccac gacggaccag gtggcggggt caaggtcgcg ttcttcaggg cggacggcaa
    10381 gccggtagac cccgaaacga tagaccgcga ggtccacgac gaggagatcg aatacatcag
    10441 gagctacctc gccaggtacg tccccgacct cgacggtgac ttcctccacg ccaagacctg
    10501 catgtacacg aacaccccgg acgagcactt cgtgatctcc acgcaccccc actaccccca
    10561 ggtagccatc gccgcgggct tctccgggca cggctacaag ttctgcagtg ttgtgggtga
    10621 gatcctggcc gacctcgtca ccgatggcga gaccccacat ccgatagatc tcttttcccc
    10681 cgctcgcctg gtcggcaccg cacgcggtta accgtagaga tcttgactgg ctgtgatcgg
    10741 gttcacacgg tcgaacgggc ggtatatact aagaaactat gcggaaggag tagatcgtgc
    10801 agaacgattt gatcaagtca caatccatat ccggtatcga agttgggcgc gatagcgagg
    10861 agatgtgtct cctcgcaaag ttgttcaatg gtttcgcaaa ctcgaccagg ctttcgatcc
    10921 tgctgcttct gtcgcggcga ggcgagatga aggtcgggga gctggtagac gaactcgggg
    10981 cgccgcagcc cagggtctcg gaccatctgc gctgtctggc ctggtgcggt tacgtacagg
    11041 tgcgccgcga gggccgaaac gctttctacg ccgtggccga tgagagggtt atgcagatac
    11101 tcgagctcgg cgaggaactc ctccaggaca acctggagca cgtcgaggcc tgcgacgata
    11161 tggagaaggg ctgctagaca ccgctccgga gacggagagc gtgtttacgc gccccgaagg
    11221 tcggggattc tagtagggcc agtccttgta agatatccgc aagaggtgtt tcaggcatgg
    11281 cccaggatct caggcagacg ataacctcag tatcaggcag gccgggaccg attctctcgg
    11341 cttacctgag cgtaaacgcc gacattcctg agaaccagcg gagggcttac ctggtacgcc
    11401 tgcgcgacgc catgaacgac gagggcgtcc ctgaagcctt gcagcagcgg gtcagggagt
    11461 acgtcgaaga agagacgcac ccaggggcca ggacgctcgc catcttcgcg ggcgaggatg
    11521 gcctcttcga gatctaccgt ttgcaggtcg acgttcccga gtcgttccgc tggggagacc
    11581 cgtacgtggc cccgctgacg ctagtaatgg acgagtacga gccatacggg gcggcggttc
    11641 tggacgccga aaggttcagg tactttgtgg tctcgccctt gagtaccgcc cacggagaag
    11701 aggagatcaa ggccaacggt ttcagggaga tcgacgtcca tcccagcgag ccttacccca
    11761 gaggagggac ggacttcgag cccgccagcc gccggacgga ggcccacgtc cacagcttct
    11821 acaaggaatt agggaaactc accagggatg tcaccttccg cgaggaggtg cagcgcttga
    11881 tcctcgccgg cccccgtgag aggaccgccg agtttcgccg caccctgccc aacgagctac
    11941 acgaccgggt agtcgccgag gtacacctgg aaatcggcgc gcccgagggc gaaattctgg
    12001 accacctgga ggccgtaagg gagagggcgg agtacgagcg cgagaaggag ctactggacg
    12061 agatcaggga gagcggcgtt cgcggcctcg acgagaccgt ggccgccctt caagaggaga
    12121 accgcgtcta ccacctcgcc gccctctggg aagtggaggc cgagatccgc tggtgcggca
    12181 acgatcagct cgccatacgc gacgccacac gcgaagattg cccgttctgt ggccagaaga
    12241 cccgcctgcg gccactgaca gacgtcctcg tcgatctcgc cgccgcccgc ggcgcgcgcc
    12301 tcgacttcat gcgcggcgaa aacgagaaca cggataccct gcgcgatgag ttcgggggta
    12361 tagcaggcct gacacgattc taaagcgctc ttggaaggtc taccttctaa gagcgcggcc
    12421 tcaggtcaca ggtatcaggc ttcaggaagt atccgggaaa agggctgaag gttaggaggc
    12481 gatgtgtacc gccttgatgt tccacaggtc cggagagtga tgtagctgca cgggcgcgat
    12541 tccgagcctg catcagggga gcggatgtaa tctctgctag agttcgcgga tggaggagta
    12601 tggtctggtg tgggagcgct tcgtcgatgg gcgccgtctg gagttcggtg ggtacatgga
    12661 tcccgcctgg cagacgggac acaggctctc ggcctccctc atagttcccg gagaggcgtc
    12721 acggttccac gagcgtctcg aaccgctcag ggaagcgctg cgtcctttcc cgttcgtctc
    12781 gctacacccc gaccacttca tgcacgttac gctggtattt ctcggctttc tcgtggacga
    12841 gccagaggaa gaggatgagg tctctcgcga gaggctgcaa gagatcgagg agcgtgccag
    12901 gatcgccctg tctgaggtac cagcctttac cgtcaggttc gcgaacctca acgctttccc
    12961 cggcgcggcg tttatcgagg cgcacgacgg aggtgcgctc gatggcctgc gcgacgcact
    13021 gtgcgcaagc tgcggcttga agaaacccca aggcccgccg caccttacca tcgcctactt
    13081 ccaggcgccg gacggcacgt atgcgcctga agaactcatc tccaccatag cacgctacag
    13141 ggactggccc ataggcgaga tcctggtaga taacgtggag atgaccctgc tcgaactccg
    13201 actggactac cccgagcccg agacgctggc ccggataccg ctggagtgag ctagtcagca
    13261 cacctcccgg cttcgccgga cggtttgtca gcatttcagc tagtcagctt tcgagtgcca
    13321 agtccttgct gacttgctga caagcggagg ccgttaggcc gcagcgtgct gactagctga
    13381 aagctatatt gcgaaggacc ttaggggtga acagccagcg cagcttaccg gctccggcct
    13441 tcggggcgtc gtcgaactcg atgcacgcga caccgccctt ctttatcttc atatctgcgc
    13501 ccccggcatc tccggttagc agatacacgg caagctcgtg caggccgggt ctgtgcccgg
    13561 ccagggctat ggacttcttc cctgcatagt tctccagcgc gatgaggact ttttccggcg
    13621 ggatgtcggg ttccagggcg gggagcgtct ccggtgctgg ccagcctgcc tggtcggcaa
    13681 ggatctccgc ggtccgccac gcccgttcgt aggggctgct caggaggacc tctacttcgg
    13741 ggactataag ggccagcccg cgggccgcct ccgcgaacgt ctcttcgccc ttcggcatga
    13801 gcggacgctt gctgtcatcg ggccagcgtt ccggatcacg attgtgggcg agggcgtggc
    13861 ggatgaggta gacgtacaag ctggcctacc gaacgccctt ggctggtatg acgatcgctt
    13921 tccggctctc caggatcttc ccgaacttct tccacttctt acccttggtc agggtgcggt
    13981 agggtttcga gtcgggcacg gcagaacgta ggtctttcgc ctcgcgggtg cagcgttctg
    14041 cgtagacgcc catcgtgaag gcgaccccgc gcgggacccg cgcttcaccg gtcttggtgc
    14101 cgagttccct caagtatgcg gcggtgacca cggcgtcctg gtggtcgccg agatcgtcct
    14161 gcagcgcctt tagaggcttg acgagcttct gcaccggttt gccgtagact tcagagaaaa
    14221 actcgagggt gtagcgcaat cgcttgccct tcttacgcac gtcgtggaag gcctcaggag
    14281 atgagttttc gtcgagacgc ttcgccgcct ttctccactt gcggtagcgg tccgagacca
    14341 gagccggcgc cgttgccgtg accgcctcgc ccgcgtagtc tttgccgttc gcctgttcga
    14401 gctccagctc ggccgctggt ccgaggcgca gcatctccgc gaaggaagac aagagacgct
    14461 cgtaccggac ggaatcgagg gtctctagca tgttcttgcg ggcctcggcc cgtcgcttgt
    14521 gggtgatggt caggatcttt tgcaggtact cagagctctc ctcgtccgcc gcttccttcc
    14581 acgccttgaa gcgctcgatc tggacgtcaa gatccctaac ttccccgagg gcgtgggcta
    14641 cccagcgcaa ctcttctctg agccagctgg cccgctcggg cagggctcct tcgaagacct
    14701 tcatcgctgc ccgcatcctg cgggtcggca cgcgcatgtc gtgcagctcc tcagggtctt
    14761 cgccgagccg cgtgccaggc tcgtggtccc gcatctcggc gaactgagtg cgcagcacgg
    14821 cgaacgctac ctcgcccagg ctcatggaag ggtctatgcg cgtagggccg aactcgaagt
    14881 ctccttccgg gttgagtccg caggcgtaca gacccgtttc gtacttggag atagacgctg
    14941 gggtcaggtc gaggacggac tgcatctcat ctacgaagcc aagcaggtca ggggtcggag
    15001 ccgtgccgat tccggcctcc agctccacac gagtcaggcg ggccgacgcc tctcccaggg
    15061 gtatctttga cgtgtcgagc gagatctcgc cgatgtgcac gtcctcctgg tctccgtctg
    15121 cagaaccctc gagcaggagc gcgaaaccct ggcggcgggt cttgatcgta aacagtgggc
    15181 gcaactcgcg tccgccgacg agcgtccgag atcgcccgcc gacggccccc ccggcttctc
    15241 tgagcgtgga gggtttatcg tcgctgaggg gctcggagat ctcacgccgc ctcctgaggc
    15301 tgccctcggc cggcgtcagg gatttcatcg tcgcctcgac ttcgccatct gtcttgcgta
    15361 cgcgcaaggc gtagccggcc cggtagaaac gccagtcatc ggtgtcgtag taggtgtccg
    15421 tgatctccac cgtggattca ggagcgatga ggagacccga agaaccggaa tcgtgctggc
    15481 cgagccagct ctcgaccgac tcgagatcgc cggcgtcgaa ctgccactct aactcctggt
    15541 gatccgtggc agagggttct ttcgtgcggt aagacttgct gacctgttcc gttgtcattc
    15601 gggcctcgtt tcgttagcct gtccccagct tgctccctga ggaacagagg cgcaatttag
    15661 cacggacgta gatcactagc aatggacgaa tccattacgg caactccgat gatcagaaac
    15721 cctcttcgag cagtatcgcc tggctgtcca cgtattcctc accgtccttg gggtgcaggc
    15781 gttcgaaggt gccgtcgggg ctgagatccc agccgttcgc gttgtcggcg agctggagat
    15841 cgagcaggcg gcggaccctc tcacggtgac gcttctcgcg cagagggaag gatgtctcga
    15901 cgcgccggtc gaggttgcgc tgcatcagat ctgcgcttcc caggtatatc ttctcgtcat
    15961 ccccgtcgga gaaggcgaag atacgggcgt gctcgaggaa gcgtcctacc agcgagacca
    16021 cgcggatgtt ctcgctcacg ccttccacgc cgggtctgag gcagcagatg ccccgtacga
    16081 tcaggtctat cctcaccccg gcctgggagg cttcgtagag gagctcgatg atctgggggt
    16141 ccgtgagcga gttggtctta cacgtgatgc tggctggctc gccccctcta gccttctcga
    16201 tctgctcctc gataagaccc acgatgctct cacgcatggt cagtggggcg acgagtagct
    16261 cctggtactc ctcctgctcg gaatagccgg tcaggtagtt gaagaggtcc gagccgtctt
    16321 cgacgagctt cgggtcatcg gtgaagtacg agaaatccgt gtagatgcgg gctgtcccag
    16381 gattgtagtt tcctgtgcca aggtggacgt agcgtctcag gcccgagccc tcgcgcctga
    16441 cgaccaggct gatcttggcg tgcgtcttga gaccgacgat accgtaggcg acatggacgc
    16501 ccctggcctc gagctggcgc gcccaggtta tgttcggttc ttcgtcgaag cgcgccttca
    16561 gctcgaccag gacggcgacc tgcgtctgct cgtcgcgcgc ctcagagagc gtctcgacta
    16621 tcggggagtt ggagccgacc cgatagaggg tctgctttat agcgagcacc tcggggtcgt
    16681 tggccgctgc cctgacgagt tcgacgacgg gtgcgaacga gtcgtaggga tggtagagca
    16741 ggatatcgcc gttcctgatg gcctgggtga tagaccgcgc gctccggatc tccgaaggaa
    16801 ctcttggcgt tataggggga tagagcagat ccgagcggtc gagatgcgtg agctcctcga
    16861 agtcagcgag gcctatcggg tggggagagg cgaagaccga attctcgtcg accttgaggt
    16921 taccggccag ccaatccctc aggtcgtccg gcatctcgtc tgagaccacg agcctgacgc
    16981 tctggccgaa ccagcggctc tccagctcgt cttctatcgc ctgcaggagg tcggccgcct
    17041 cgtcctcctc gatcacgaag tccgcattgc gggttacctg gaagacgtag ctagcgacga
    17101 cctccttgcc ggggaagagc tcgtccaggt tggccgagat gacttcttcg accctcacaa
    17161 agcgcatctc gggcctttcg tccggaccgc ggtcctcgtc ggcgagccgc ataaagcggt
    17221 ctatggtggt cggcaccttg acgcgggcca tcacccgcct tcccgcgtcc tcgatcacga
    17281 cgagcaagtt cagcgatagg ttggagatgt gggggaaggg gtgcgccggg tctatggcga
    17341 ggggcgtgag tatgggcagt atgtccctga caaacctctc cctgagctcc cgctgctcgg
    17401 cagcacggac cttgacgtgg ggcaccgtgc gtatgccctc ttcttccagg gcgggtatta
    17461 gctccctgtt cagtatcttg cgccgctcct cgaagaattc ctccgtgtga tcgtgtatcc
    17521 gggagagctg ctcctctggg gtcatcccgt ccggaacggg gttcgggaga tccgaagcga
    17581 cctgctgttg caaccctgcg acccgaatca tgaagaactc gtccaggttg gtctcggaga
    17641 tcgcgacgaa ccgtaccctc tccagcagcg ggtgtcgctg gtcgcgggcc tgttcgagga
    17701 ccctgttgtt gaaccccagc cacgagagct ccctgtttat gtacagggaa gggtcggaaa
    17761 ggctcggcct acgtgctacg tcagtcatat ctctcccctc cgcgagcagc gtcccctcct
    17821 tacctcctgc gcgcgctacg gtcagatcct tgccaaagcc atcgcgtcgt gtaacgcgta
    17881 gtgaaacctc ataccgccca tgataccgtc ccacggcacc gggtgatata ttcgaacctc
    17941 ctaggagggt tgtcgatcgt atccgctcgg cgcgagccgg cgtcagaagt ggaggaagcc
    18001 atctcgaaga ggggcaaaaa gaaggcgaag aagaaagggc tgaagggtgg cgcgctggcg
    18061 acgctcgcaa agccggccgc gaaggtcaga gactacgacg cccacgtgct catctgcaag
    18121 gacggcgatt gcaagaagcg cggatccaaa gccgtccgca agtcgctcaa gaacgagttg
    18181 cgcgcggagg gcatacacag ggacgttcgc gtggactccg taggctgcct cggcctctgt
    18241 aaacatggcc cgaacctcgt cgtctacccg ggtggcacct ggtacctcgg cgtcgtagag
    18301 caagaagtgc cagagatagt acagagacac ctcaaggatg gcgaacccgt cgaacatctc
    18361 gccgccgaac tgagaccccg caagagagcc aaatagaaat cagcgggtct aagcccttgc
    18421 aagagctagg ccaccagcgc gccgagcggc attacattcc gcctagcgct aacccacggc
    18481 tgtcaactcg gcctggacga tcaggcgctg cgcgtagtcg ggcagggtac acgtctgcag
    18541 ggtgatgacg ctcttgcccg ggacggggcg tgtgacgtag tactcgtcgg ggttgatgat
    18601 ggttctcttg aatacgttgt aagtgtaccg ggtaccgttc gcgtcggtca ggatgacctt
    18661 gtcgccatcc cccagcttgt tcaagtccca gaacacgaga taactcctcg tgcccgggaa
    18721 gcccaggcgg tggcccgcga tatagacgtt ggcccccgtc tgccagggga aaccggtgcc
    18781 tttcacgtgc agcgtcccgt cgtggagtgc ggccttgtca ttcgcagcac ccgtgtagac
    18841 gggcacgcgg tcgacgcgcc gcatctcggg gatggtcagg gtcatggtcg agtcgctcgg
    18901 gggcttctgc gcaaagacgg ccgcgctggc gaacgggtcg cgtatgagga aggagtaccc
    18961 catcagaacc aacccggaca cgacgagcaa ggtggccacg aggtagccaa atatcctgag
    19021 cccgcttctt ctcgtcctgt aggtcctcat ctccgtctac cccctctccg tagatacggc
    19081 tccttctccg ccgtgcaggt atccgcggcg aaagtaactt ccaggttaca aaatgggccc
    19141 tgccggtgtc aatcgagcac agggccctgg ttggggctta ccctttgccc ttcagctcgc
    19201 cctgcactat gaggcggtgc gagtagtcgg ggagcgtaca ggtctggagg ctgacgatgt
    19261 tcttgccctt cgtcggccgc atgatgtcaa cggtgtcggg agagatcacg cgcttctcga
    19321 agacctggta ggtgtaacgg gtcccgtctg cgtcggtcag gaggatctcg tcgcccttct
    19381 ccagcttgtc gaggtcccag aacacgaggt cgctcctggt accgggataa cccaccctgt
    19441 ggccagcgat gtagacgttg gccacgtcct gccacgggaa tcccgtgccc ctcacgtgga
    19501 ccacgccgtc gtggagggcc tcctcgtacc cgctcgcggg ggcgtcgtag acgggcacgc
    19561 cgtccacccg gtccagatca gggacggtga gttcgagctg cttgctctcc ggcgtcctgg
    19621 cccgggaacc cactagggga tcctgtgccg tctcgacgat cctctccgga atactcctgt
    19681 tgaacttcct ttcgagctcc tcggagatcc ggccgtgtac acggtcgggg ctatcggccg
    19741 aggccggagc acccccggac agcagagaat acccaaggag accgattccg gcaaaggcga
    19801 atacggccac cgcgagcgtg gccagcctcc acttctcgta ggtggtactc agcttcttca
    19861 tcttcctcga cccccttcct cgatcagata ctatggtcga tagtgaaact ttaagcccca
    19921 tatgtgacat ttcgattaca aaacggggtc ttgttggtaa cgactggtcc ttggaagcgc
    19981 agatccggca ccgacgctat aatctggcgc gtgaaagtag ggatacccaa ggaggtggcc
    20041 gagggcgagc gcagggtcgg gctagtgccc gagaccgtgg gcaggctcgt caaggacggt
    20101 ttcgaggtcc tcgtggagtc gggggcgggc cgcgactaca acctcgacgc gaactacgag
    20161 gaggcggggg cgaagatcgt agggggcgcc gatggcgtct acggcgaagc cgacatagtg
    20221 gtcaaggtgc aaagcccgag ggaggacgag ctcgggctcc tgcgcgaggg tcaggtcctg
    20281 atctgtttcc tcaacggacc gcaggagccc gagctcgtag agaagctcgc cgaggcgggc
    20341 gtcaccgtct tctcgaacga ggctgtcccg cgcaccagcg cggcgcaagc catggatgct
    20401 ctctcctcga tgggctccat cgccggctac aagtgcgcgc tcatcgcggc cgacaccatc
    20461 ggcaagtatg tccccatgct ctccaccgcc gcaggtacga cgaaggctgc gaacgtcatg
    20521 gtgttcgggg tggcggtggc ggggttgcag gccattgcca tcatgcaccg cctcgggggc
    20581 gaagtgttcg cctacgacat caggccagag accaaggagc aggcgcagtc actgggcgcc
    20641 acgtttatgg actccgagga cgagaaggag gaggaaaagg aggacccgtt cgaggagtac
    20701 cagcccacag gatggcgcaa gctgatgacc tcactcggct tctactccta cgccgaacca
    20761 ccccgggacc agtacatcgt cgagggcgaa gaggaggaag aggctaactc cgaggaggaa
    20821 gacgaagggt ggtcccagga gaagctcgat gaggaccaga agctcatcag ggagcgcatg
    20881 gaggagatgg acatagtgat cacgaccgcg ctcgtccctg gcaggaaggc tccacagctc
    20941 gtggacaagg acatggtcga gagcatgaaa ccggggtctg tgatcgtgga cctcgccgcc
    21001 gagagcgggg gtaactgcga gcttaccgag gtcggggaga ccgtcgagca cgaaggggtc
    21061 aggatcgtcg gtcccgtaaa cctgccgagc acgatgccca tacacgccag ccagctctac
    21121 tcgcgcaaca tgtacaacct catcgggcac attaccgaag accgctcgga ggacaaagac
    21181 gagaaggacc tgcacctgga tctggacttc gaggatgaga tcaccggcga gacgtgcatc
    21241 gcgcacgggg gcgagatccg ccacgggccg accagggaag cgctccagga ggccggatga
    21301 cctcgcaact gctcgcccag ttggcgatct tcgttatctc ggcgttcctc ggattcgagc
    21361 tgatctcccg cgtgccgaac ctcctgcata cgccgcttat gagcgcgacg aatgccatac
    21421 acgggatcgt tttggtaggg gcgatcatcg cgctcgcctc cgcctctgac ggcttcacca
    21481 cgaccgtcgg catgatagcc gtgatcttcg gggcgatgaa cgtggtcggc ggcttctggg
    21541 ttacgaacag gatgctcggc atgttcggcc cgccgaggaa gcgggtcaag aagagagagg
    21601 aatagccctt gcaggtcgca ccgttcatcg cctacctcgc ggcggccgca ctgttcatct
    21661 acggcatccg gcgcttgagg acacccgaaa cggctcgttc ggggaacacc ctcgcggcgg
    21721 tcgggatggc catcgccctc atcgcgacga tcttcgtcgc cgacatagac aacgtgttga
    21781 gcgtcggcca gatctcggtc ggggtgctga tcgggtcggt actaggggcg gtcgcggccc
    21841 agcgggtaca gatgacggcg atgccgcaga tggtcgcggc tttcaacggc gtgggtggcg
    21901 gagcggccgc tctcgtcgcc gtctcggagt tctaccgggt ggaggcgctc ggccagggcc
    21961 acgagacgct cgtcgccacc atagctgtgc tcctcagcgt cattatcggg acggtgagct
    22021 tcgcgggaag caccatcgcg ttcatcaagc tccaggagct ggcgttgacg ggctcggtga
    22081 cgttcccttt acagcaggtg gtgaacgccg ccatctttgt tggcattttg cttctctccg
    22141 caaacatcct ggcgggcggg ctcgtgctct cgccgctcgt ctctctgtac ttgatcctgg
    22201 gcgcttcgct gctgctcggc gtcctgttcg taattcccat cggcggggcg gacatgccca
    22261 tcgtgatctc gctgctcaac gcgttcaccg ggctcgcggc ggcggcctcg ggcttcgtgc
    22321 tttccaacta cgccctgatc atcagcggta cgctggtcgg cgcttcgggt accatcctga
    22381 ccaggctcat gtcgggcgcg ctggggcgtc ctctgggcaa gatcatcttc gccggcatcg
    22441 ccgcttccgg gggtggcgga agcgagcagc aagaagaccg ggaagtcaac gacaagagcg
    22501 ccggggaggt gggcgagtac ctcgcggacg atgcggagaa agtcatcatc gtacccggtt
    22561 acggcctcgc cgtagcccag gcccagcagg ccatgcgaga cctggtagag acgctggagt
    22621 ccgaaggcaa agaggtcctc ttcggcatcc atcccgtggc ggggcgtatg ccaggtcaca
    22681 tgaacgtgct cttgaccgag gtcggcatac cctacgagaa gctctacgac ctcgaagaca
    22741 taaaccccga gttcgacgac gccgacgccg cgatcatcgt cggcgccaac gacgtggtca
    22801 acccttcggc caacgacccc gaagtggaga gtccgataag cggaatgccc gtgctcaccg
    22861 tcgagaacgc ggagcacgtc atcttcatca agcgctccct gagcccaggc ttcgcgggcg
    22921 tggacaaccc cctcttctac gacaccgaaa aaaccatgat gctcttctcc gacgccaagc
    22981 aaggcttgca ggacctcaac gaagccgtaa agaacagcta gcgcacgctc ggaataccac
    23041 accgcaccca ctaaacagaa ccatcatcag ggcgactaaa gtgggcgtga cctaacctat
    23101 taccttcaac tgcctgactt tgcatcgtgg caccgaacta ctcgcagttc cttttccgac
    23161 cggtgggtgg agtacgagat gccccactat ttccgtaacc gtcgaacgtg gagacctact
    23221 cgtaaagcgc acgaaggggc tgcaaagctc tacgagtatg cgcacagcgt agagggcggc
    23281 cgttaccaga gatggccata gagtggctat ctttgctgaa gagggtgctt gtggttggag
    23341 gtgagaaacg cttgacgcag tggattcccg cgtaagcggg aatgacggac gaggaactga
    23401 gaattaggag tttggaatgg tccagaacgt ggccctttcc gaactcacaa ttccaccctc
    23461 acaattcaag agcgatcaag agcgatcctg cgggttgcgt actatcggtc gggaaggtgg
    23521 gaggctacct gctggcggga ggttaggttc agcttcttta gtgtgttggt gacgtggtgg
    23581 tgcaccgtgt gctcggagag gaagagctcg gaggcgatcc ggcggttggt cagccctctg
    23641 ctcacaagga tcgctatttc ctactcccga cgggtgaggc tggactgtcc atcagacgag
    23701 gattgctccg agtcgggaga tggaggcatc gtgggctcct ccgttagcaa tgataacaat
    23761 ggaggtagat cgggactact ctccggcgaa gcaaggtcca cagacggagt cgacagcttc
    23821 cttcgctttg ttgggaatcc cgtcgccggg ccgggtagcg ccgacgggac ccgcttgcgt
    23881 ctcgtgaaga ctctggctgc gagacacgcg ggcaggtgga tccgccgggg cgaccatgct
    23941 ttccccgccg cgctgttccc cggccgccgg ttctggccga accttggttg gcccgggtgc
    24001 gaccgtcgca ggcgctgagg cagacccggt gtaagcgggt gacgcgctac tcgctgaagc
    24061 cgccgatgcg ctcgctgacg cgattgccga ggatgatggc gcgtgtgccg aacggggtgc
    24121 gtgctcctct tgcccgcttt gctccgctcg ggtttcgccg tccttggagg gaggttcggc
    24181 ctgtttctcc tcgctcaaat cttccggatt acgttcagcg tcttccacgt tgccgaccaa
    24241 gacgatcccg gagggaagct gggagaccgt cggctctcgt actggtgaga ccggcggtag
    24301 atcttcactc ctgatgctgg atgcgaggta ggtggcgagg acgaagagca gaactaccac
    24361 aaggctcacg tacaagccgg gtctgcgaac gggcgccatc gcgcctgctt cgtcttccac
    24421 cgtcccgccg atgccagcca gacggcccag ggatgccagg aacccacgcg gctcatccga
    24481 cggagaatct tccggcgcct ggtcttgttc tggttgcagg gatgcctggc ggcgtttgag
    24541 ctcccttgct atcgccgctc cgtctagtcc aaggtgctcg gcgtaggtct tgagggaccc
    24601 gagcatgtac acggcaggga gcacgtcgaa gttctcattc tccaggtcac gcaggtacct
    24661 ggtccggatc ttcgtcgcct cctcgacctg ctggagcgat agacctcgct tctcgcgggc
    24721 cagcctcagg gtcctgccta tctctatccc cgtatgcggg ttgccagcct tgccgctcat
    24781 gctcacccag gtccgtctaa gtctgctccc cccgggtaga ccatcgtcgc attctcctat
    24841 cttggaatgt atcacacgcc acaggatttg ggcgcacgtt ttgggcggtt cgcacgttcg
    24901 aaaatccagg taggggaggg tttgaaacag gggagggttt gaaaccctcc cctactcgac
    24961 gatgggctgg gccaggatcc agaggctagt catcgtgtac aggaccataa ggacgagcat
    25021 cggcagttgg ctcttcaaag cgcgtccagg gtcacgaagt aggcgcagtg agatcgagtg
    25081 tgcaagatac acggcgatca cgtgtcctgc cacgatgagc gcgacctgcg aataccagac
    25141 gaacccggcc ccgacgatgc cgtaacgggg ctcgaaatct gcggtgccga agaggttcca
    25201 gtcccatccc agaggatcgg agacgaggga gactatcatc tgtccctgga cgagcaggta
    25261 cgtgtagtag tgggctgcct ggtaggcgat cgcgatgggt accagcgaga aagcatacgc
    25321 ggccgcgaac cgcctgatac ctatcccccc tccaccgcca aagactcggc tcagttccac
    25381 aaacccgaga tagatcgcga ggaatagcaa gggcagcagt atcgtcccca gcgtctgcgt
    25441 cacgggggta agacgtacga tctcgagcca cagtggggtt tcgagcaggc cgtcgaaagt
    25501 cacgccggca agcaccacga tcacgaacgc ggccccgccc ggcgtaacct tctctggcag
    25561 tccgagaccg accgcgggag ggcgcaggtt caattcgcgc tcttcgggcg cggcgcgggc
    25621 gaagcactcg taacagttca cgcaccctcc ccttgcgtcg tcgccacagc cgtcgcagcc
    25681 caggcaaact tcggcgtcct tcaccctgac ttctgtgggc gcgaaccggc cgagcaggcc
    25741 gaagaacacc gagaacgcat ctccccgacg caaccagatc tccttgccga agaaggccat
    25801 gccgtagagt gtgatcaggg agtaagccag ggcgaagagg gcgatgtagc gcggcacgta
    25861 cgaacccgag aagacgttct cgatccagac gaatacgagg tagagcccta cagctggcca
    25921 gatgccgaga gcttcggggt agggctcgcc cagctcgagc ccgtccctgt agccgagcct
    25981 gcggaccagc ctttcggccc agtcgaagac taccctccag gggtttacga gcggccacag
    26041 gttaccgacg aaagcggtaa agaggctgag gccgacccac caaataatcc acacgaaagt
    26101 cggggcgaag ttgaagccgc tctgcagacc aaccaggccg gtgatcagga cgaccaggaa
    26161 gagggcgaca gagaacaagc gcaggccgcc tatgagcaag ttcgaagtca ggagcgtcct
    26221 cagtagccga acgcgaaaca agtcaaaacg gggataatcg tatgcggagt accgctcctt
    26281 tcgggagaac aagatcagcg gtaagaagga gagcaccacg gcggcggcgg cgccgtagag
    26341 gtagagccag agcgggatcg ggatgtcata ggtcggcccg aagccgtggg ccagcgcggg
    26401 acgggcgccg gagagtataa gtgagaacgc gagcgcggca actgcgacgc gacgcatcat
    26461 cccttcgcta ccgcgggcgc acctgtagga cgccgagttc cctctcggat ccgtggtctt
    26521 cgatctcgaa gcgtcccgtg agatccgcct cgaagcgaag gcttgccttc tgtcctggcc
    26581 cgacctcccc ttctacgtcg tagccatgca ggtgtaactc catcggccta tcggagctaa
    26641 cccggagcgt cacgctatca tcctcgttga ctgagatctc ggccgggctc atctctccgc
    26701 cctcgatgga gacggcgata gtcctctcgc gcggcacacc ggcaggggtg tcgggacgca
    26761 gcaggaagaa gagcccgccc aggatcacta tgatcaccgc gatcaggatc atgcttctgg
    26821 ccaacgtccc tctatcctcc gtgagatcta gttctcgtca ctatcccggc tcgactgtag
    26881 tagagggcac cgtcaacgtt tctcgcgctg tctggtcgcg tacctcataa ggccgtcggt
    26941 cagcatactg taggagccgg ccaggccata gcgctccgac tcggtaatgt cggagccttc
    27001 ggcgagcatc tcggcatccc ctttggccct tagctggtcg gagatctcct cgcggcctga
    27061 gccttcatcc atctgccctt ctacgaacag caaccagtcc tggaggcgct gctcgagctc
    27121 gcccagatgc cgccccacat cctcgtagct accgaagtgt gtgagccaga ggcatgcagg
    27181 atctatctgc ctcatgtagt tgatgctctg tatccacgcc tctacatcga tctcgggcgg
    27241 aggcgtcggc ggacggatgt aggactggcc aggcagccgg ataccggcca catcaccggt
    27301 aaagagcgcc cctgagtcag gctccaggta ggcgaggtga tggtaggcgt gacccggcgt
    27361 gtcgtgggct acgagaacgc ctcccgcggc ctccgtctcc accccgtctt tcaggactct
    27421 gatcctatct tcgggcacgg ggcgtgcctc gccccagagc tcgtccatcc tctcgccgta
    27481 gatgcgggtg gcgctcttca ctagcttcga agggtcgaca tggtgcgggt agccgacctc
    27541 gtgcacgtag aaagtcgcgt tgggtagcat cccggagaga tgccccgagg cgcctgcgtg
    27601 gtcgaggtgt atgtgcgtaa ggaagacctg ccgcacgtct tcgtgggaga cgccgcggtc
    27661 cttcagaccc gcggtgagcc ggtcgaggca attcgtcgga ccggtctcca cgagggcggc
    27721 ggaagccccg tcctcgccga gcaataagaa cgaggctatt acctgcccgg tatccatgaa
    27781 ttcaaggtct atcgtctcta tcttcatcta cgaccctctt tgctgtggct ccaagcggag
    27841 attatacaga ccctctttgt tagacttccg gcgcgcctcg aaaaaggaaa ggactctgac
    27901 ttggatctca acctcatgcg agagctatcc gtcaagaccg aaacgaagat cgtcctgctc
    27961 atcctggacg gccttggcgg gttgcccatg gacccatcag gccacacgga gctcgaggcc
    28021 gcagagaccc cgaacctcga cgccctcgca gcccggtccg acctcgggct ctcgcaaccc
    28081 gtcgcagcgg gggtgagccc agggagtggc ccgggccatc tcggcctctt cggatacgat
    28141 cccgtccgct tccaggtcgg acgcggcgtc ctcagtgcgc tcggcgtcgg cttcgacctc
    28201 ggcgagagcg atctggcgac ccgcgtgaac ttcgccacca aagacgaatc gggcaggatc
    28261 tctgaccgga gggcgggccg aatcccctcc gagaagggcg aggagctagt cgcgctcctg
    28321 aacgagaagc tgaacctcga cggcgtcgag atctacgtcg tgcccgagaa ggaatacagg
    28381 gccgtcgccg tcttcagggg cgaggggctc tccgacgccc tggccgactc cgacccccag
    28441 cgcacgggtc tcgaaccgct ccccgtcgat ccgacggacg ggtcaccaga ggccaagaag
    28501 agcgcgcaga tcgcaaactc cttcgtcgag caggcgaacg agatcctggc cgaccagcac
    28561 ccggcgaaca ccgtcctcgt gcgcggattc gggatgcacc cagccctccc ctctttcgag
    28621 gagacctaca ggctcgacgc ggccgccatc gcgagctacc cgatgtacaa gggactggcc
    28681 cgcctcgccg gcatgcatct cctgaaggag ggcgaaggca tctccggaga attcgagacc
    28741 ctgaagacga actggggaga ccacgacttc ttcttcgtcc acatcaaacc gacagacgcc
    28801 gcgggcgaag acggggactt cgacaggaag gcctccgtga tcgaggaggt agacgcccgg
    28861 atacccgcgc tgctagacct cggccccgac gccctcgcca tcacaggcga ccacgcgacg
    28921 ccggcgaagt tgaagagcca ctcctggcac ggcgtgccgt tcttgctcgc ctcaccatac
    28981 accctcccga ccgcggatag cttcggggag cggacgtgtg ctggaggctc gctcggcgtc
    29041 ttccaggccg aagagattat gggctacctc atgggccacg ccttaaagct caaccgctat
    29101 ggcgcttgag cgcacgctgc cttcggcagc ttgtcagcac gccgcctccc accggtcggc
    29161 ggctctcagc acttcagcaa gtcctctgag atcctcatgc gcggcagagt gaggccggaa
    29221 cgggagatgc tgacttgctg acgagcgagg gccttatagg cccgaagcgt gctgaaatgc
    29281 tgacaagcga aggcgaagcc ggagcgtgct gaacagcgag gaactcgctg ttctagagcg
    29341 ggagatcatc tcctgtcgtc gctgcccgcg cctcgtt
//
LOCUS       DATMVF010000107        16737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_608036, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000107 DATMVF010000000
VERSION     DATMVF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16737
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608036"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..847)
                     /locus_tag="VN178_05150"
     CDS             complement(35..847)
                     /locus_tag="VN178_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWS80391.1"
                     /translation="MDDSAQQRLFAETPGSIEPPEEGLYLGTSGWSYADWEGTLYPEA
                     LPSASRLAEYAKHYATVEIDSTFYGTPRRSTVQNWREVAPDGFLFAAKFPKEITHDRN
                     LVGATAETDGFLRTMAELGDRLGPLLLQLPPSFDVEGMGVLEDYLRRLPEGFRFAVEV
                     RHRSWIGSDLPEMLSERGVALTLIDYPGMPRMERATAVFSYIRWLGNRREFPEGHTHL
                     KKNRDDDLSWWSELVNGFLGEGRTVFAYANNHYQNHSPSTLERFLEIRRGEC"
     gene            1049..1975
                     /locus_tag="VN178_05155"
     CDS             1049..1975
                     /locus_tag="VN178_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWS80392.1"
                     /translation="MPDTMKVFYSDHFVLLLPEGHRFPMVKYSKLRERVERDGICGPG
                     ALSTPRAVTDEEILRAHAPDYLKRVVSGTLTQKEMRRIGFPWSERMVERSRRASGGTL
                     SACLAALDEGFAANLAGGTHHAFSNRGEGYCVFNDSAIAARAVQAAGSVRRVVIIDTD
                     VHQGNGTAAILAGDPTVFTFSIHGAKNFPFNKEVSDLDAPLPDGADDSEFLGTLERGL
                     RVALDAADADLAIYLAGADPFEGDRLGRLSVTKSGLAERDRIVLETCREREIPVAVTM
                     AGGYANEVEDTVDIHFQSIRRAADLLESKGDR"
     gene            1989..2984
                     /locus_tag="VN178_05160"
     CDS             1989..2984
                     /locus_tag="VN178_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS80393.1"
                     /translation="MSEDQTSCVIVGAGISGLLAAKTLNEEGWTVTVLEKSRGVGGRM
                     ATRRIGSGKFDHGAQFFTVRGERFAGLVEDWLSAGVATEWTRGFADPEGRPNEDGYPR
                     YRGTEGMTSIPKYISRGLDVRTGERGVRVDAADDGWEVVCESGSRVAGNALVLTAPVP
                     QALDLASSGSYIPPDGARRQLEAVSYDPCLALMVLLDGSTGIPEPGGMQIKGEPIDWI
                     GDNRRKGISEAPAVTVHAGPEWSRSHFENDETEITVSMISLAGKRLDTDLVPSVVVTS
                     LARWRYSWVTNPHPDPCLLASDDPPLLFCGDAFGQPKVEGAALSGLAAADHLLGR"
     gene            3110..3457
                     /locus_tag="VN178_05165"
     CDS             3110..3457
                     /locus_tag="VN178_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80394.1"
                     /translation="MGNRRVALKPHASKIRQWVEEGRGDTWIAQELNTTPSSVQSFRS
                     RNSIYRRDPVRRGQLSEHAAVLEETEDGIVLKTDARDSEVFSREWRHYVHGSPEDLQV
                     VITQDRIYLEKVR"
     gene            3459..3749
                     /locus_tag="VN178_05170"
     CDS             3459..3749
                     /locus_tag="VN178_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80395.1"
                     /translation="MPGEPAVGLRLPLEDAEALHALLERLLESGQQDPRLEHSYRLLG
                     WRILAAKGGRGLTGRMANLARQADSLEEYEAALERELGPVLDGLERGENRDP"
     gene            3918..4928
                     /locus_tag="VN178_05175"
     CDS             3918..4928
                     /locus_tag="VN178_05175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWS80396.1"
                     /translation="MQYLLSSLATMVFIGILALLSQWSRKSRRAEISLLIVLVFLSLL
                     LFATGALLGIIWVSGQMPPDAYPQPLLAVTAVPVTLSGLIGLALCVPTLLKIVRGRPN
                     GEFWTDPPIFLALWLFATVLLANNVVGVLGFEQLDQVDAFSLGTGGRIPPVAILASQL
                     PFVVIALLGVGAGIRRGPRETLARLGYGPISLKQIGIVVLFIAGAFALSVASDALFSQ
                     LQPDLYRRVGEVSQRLFDPKGLSLAAAVLFALLIGVGAGLGEETLFRGAVQPVLGIPV
                     TSVLFASMHVQYGPSLLLGYIFVLSIGLGLLRRHVNTTASFLAHAGYNTLGILAVYFF
                     GW"
     gene            complement(5031..5432)
                     /locus_tag="VN178_05180"
     CDS             complement(5031..5432)
                     /locus_tag="VN178_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80397.1"
                     /translation="MRFALKQITPSDARTISCWRYEEPYSIYDGDPTSVDAILQPRSS
                     YHSVHDESGDLAGYFCFGEDAQVASGKRLGLVETEPALDVGLGMRPDLTGRASEKGSY
                     TPASASPGRPTHPRRSASPWSPSTTAPSASM"
     gene            complement(5451..5750)
                     /locus_tag="VN178_05185"
     CDS             complement(5451..5750)
                     /locus_tag="VN178_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS80398.1"
                     /translation="MKTLEMSPERHAGDLLSTIGETLRAERNERGLTLKQVAEGAHVS
                     VSYLAEIERGEKDPSSRVLESIASGLDVEVSELLVRIASALEPAPVVPVSDSLAA"
     gene            complement(5761..6639)
                     /locus_tag="VN178_05190"
     CDS             complement(5761..6639)
                     /locus_tag="VN178_05190"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HWS80399.1"
                     /translation="MPERTNRIGRHLPTSGGLAKTLWAARKLGCETIQIFVSNPQAWA
                     DPRRRQDAETFVEGSLEMGISPVVAHAKYLINLASTSEEQREYSVRALASELSAAGAL
                     GVDLVVVHAGSHGGAGEEKGMERLVDGLGRARERAASLDGGAGVVAEPVVENSVGAGT
                     QLCSTFEELREVARRGGVRVCVDTAHAFVAGYDLSAESGGRDVAEELVDALGDSVALV
                     HLNDAKNELGSHRDGHRKVGEGRIPLDAWPGFFDGLPGVPVVMETPYGTPEADSEEVL
                     LVKKLAGGLRKAPVGR"
     gene            6728..7174
                     /locus_tag="VN178_05195"
     CDS             6728..7174
                     /locus_tag="VN178_05195"
                     /inference="COORDINATES: protein motif:HMM:NF027852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HWS80400.1"
                     /translation="MAVEGKVAKILGNNEIVINRGRGEGVRQGMLFEIFAPEGEEVWD
                     PDTGETLGTVEDVKAKAEVTEVKDRLAVARLQNAQDTPFGAVNIGEMQENLQRMFGQM
                     FGEGVQIRGFGATSGDDPDLESMLGGPLQDLSQVQVGDAVREIKRR"
     gene            7197..7892
                     /locus_tag="VN178_05200"
     CDS             7197..7892
                     /locus_tag="VN178_05200"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWS80401.1"
                     /translation="MNTLSRLPVTFGLIAACVTVYAAVAIAGQGYGYPLNVGLVTQPP
                     EVLARGALVPAAVAGGQVWLLLSSMFLHSGFIHLALNMLSLYFLGSFVEQAFGKSRFL
                     ALYLCSGLAGGIAYLYFGAFEVPAVGASGAIFGLLGGVFGYSVRRGTFSWQNPLIRQL
                     LILTALNLYIGFSVPNISNTAHIGGLLGGAAFGWLTAPTVYSGKPLRAATPTVILFGA
                     EIVLLAVWLLVFA"
     gene            complement(7939..8664)
                     /locus_tag="VN178_05205"
     CDS             complement(7939..8664)
                     /locus_tag="VN178_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWS80402.1"
                     /translation="MNAILMASLAAVGFSAASLLALLGGELGVRGRSYAAAFAAGILL
                     ALAFVDLFPESLELGGEAAVAGFVGGFALLFVTEAFTRAHTHHLPDEHVHKHALGPFV
                     LGLAVHNVADGFVLGVGAKAGAVTSWLVGLGVLVHQIPVGISLAAVLIAARVGRAGVM
                     RVAVLLGLAIPAAAALTVAFPAPGEGALGLLSGVAGGVLAYIGAAHLLPEAQAEDTNR
                     LAGIVFVVTLIVVSVGLIAIQHG"
     gene            8737..10716
                     /locus_tag="VN178_05210"
     CDS             8737..10716
                     /locus_tag="VN178_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWS80403.1"
                     /translation="MAVTFCEIFVNSLELVASGHHKQLTLFARFRSILSGRDWVYLSG
                     LLVPFVVYNLVLKGLRIDSQEELPGGFAAFALIRSDLLFNLGYVFLWVGLFALTRRSR
                     LRWPVVVLFHTVTIAVIATTTSAHGYFQETGSTLSLSVITYSLGSLGEITDVIGSVTS
                     LAIWAWVLVILGYIVLGPWIIATLVCGKPGEPDSFGSPSTSRLFALGAFTMSIGFIFL
                     SLPVGAGVAGKAFSRDAVVNIVMSEVEHARIRALVSNAASMEPPVDTRLQKTSETEKK
                     NVVLIHLESARARSMTPYNEDLDTTPYLDTLAKKSLLAERAYTIVPHTSKAVTSVNCG
                     IDAHLVREITEVEPGGVPARCLPELLKEQGYNSVWFSSATEHFEDRRDLVENFGYEDF
                     LPVETMETAGFQKSNYFGYEDNIMLRPSREWLEQHNDEPFVATYLGVTGHHDYRPIDR
                     YGLKDYSNLPGLDHYQNEMRYLDFFVRNVMNQYKELGLYDDTIFVIYGDHGEGFGEHD
                     LYQHDNTIYQEGLKVPFIIHAPGRFAGGERVETLSNQLDVLPTLFDMLGYKVTGGQYP
                     GRSLLGAPEGNRTLYFSCFDEYRCLASIKGTKKYIYFFGNQPEEVYDLASDPLERKNI
                     VGELSRKELKDKRLQVLAWYSKVNAMYEKREAKQE"
     gene            complement(10727..11551)
                     /gene="lgt"
                     /locus_tag="VN178_05215"
     CDS             complement(10727..11551)
                     /gene="lgt"
                     /locus_tag="VN178_05215"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWS80404.1"
                     /translation="MNLLSLPSPPSPLIFEVGPFALRYYGLCIALGIIVATWLTGREL
                     ERKGYDSALALDSLFFIVPLGFIGARAYHVLTDYGLYAGDPFPGVFEVWNGGLGIYGG
                     VVGGFVGLLIFSRIRGISPWAFADAVAPGLVLAQAIGRWGNYFNQELFGRPSDLPWAI
                     RIAPENRPVGYSDAAAFHPTFLYESIWDVVVCLILLWVARRFADRLKNGDVFLLYVSL
                     YSFGRFFVEALRVDPAFLIGGTIRGNLFVSSLLALGFAMILLLRHSGSRRKRPESS"
     gene            complement(11553..13682)
                     /gene="mrdA"
                     /locus_tag="VN178_05220"
     CDS             complement(11553..13682)
                     /gene="mrdA"
                     /locus_tag="VN178_05220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWS80405.1"
                     /translation="MREKKQANSAKKRVESPPMTKGNMQVRVVTLAALIAAVFLILGA
                     RLWYLQVLTGQDYTLAARATQHTNIKIPAQRGVVYDRDGKILANNVPGLNVTVIPDEI
                     SRDKVRELATVVGADTEAVLGRYDAAMETGSQYSPILVKENAGRDAVTYVSERAGEFT
                     GVAVNDDWVRSYPEGRLAAHILGYTGAVNHDELKQEPFKGLPADSIVGKSGVELSYEE
                     ALRGKAGQRTYNVDALGRIVPEGSRVDSMGRFVDENGNPINVNPSLELPDHVTEPEPG
                     NNLTLTIDIDLQRVVEKELDAALERTWDAGYDGRGGAVVAMNPENGEILAMASRPDFD
                     PQLFVGGISGDRELAAYNYLASDRSNDPFLNRAIVGGQPGASTFKIFTGMAGLAAGVI
                     DAYTIVNDTAAMSGTGCWMPSSVSASPCYRSWRQNSPNYHFLGDHGPQNISEAIADSN
                     DIFFYQVADWIWNQTRDKDWLPHYYERWGFGRLTGIDISGETEGRIPTEQGERELHKA
                     LHGSTEGAYWSVGDWVNLAIGQGDLLVSPIQMARAYSALYNGGTLVTPHVGKEITDQN
                     GNVVETFSPKPAGRVDTNQYQIDLVIRGLRGVTEEDGTAGPVFVDSTLPFAGKSGTGE
                     LPAGKDYVNWFAGWVEGREEQPLVVVTMIEGGGTFEEGSEMTAGPVVRHILEAYYGVE
                     QSPKDPYRTDSPPRDHASTESESAPVE"
     gene            complement(13724..14476)
                     /locus_tag="VN178_05225"
     CDS             complement(13724..14476)
                     /locus_tag="VN178_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWS80406.1"
                     /translation="MRPRRRSARPRREGTIARITETAINVLSLLVVGVGVVVTASFFV
                     GSPQHDAEAEVAVAEKVSTASRQPRLPVAAGKIDKAIERQKRETQRKDPDGTAARKRA
                     VDQGPESRMLRMTIPELAQIRNDTVPYTVSDDKKAFRKHAAVHLRGTGNPWDRQANVY
                     IAGHRLGFPGTDSWLSFWDLNTLENGDKVFIIDAAGKRYVYKVFRTFVVDPDDVSVTR
                     PLKGRNIVSLQTCTLPDYSRRLIVQAEKVAER"
     gene            complement(14505..14975)
                     /locus_tag="VN178_05230"
     CDS             complement(14505..14975)
                     /locus_tag="VN178_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80407.1"
                     /translation="MLAAVVSLWPVNFSPGEELISPGARDIAVSQEDRVYPTSDRLRF
                     GGGAEEVYVYLRVEDFAAYEDLEARVGRTSRVSALGRLFGGGGELVVSKEGEERLSVT
                     YGRASGVVKFAVRSGSGRRLPSGDYTVAVYTVSGENGGNSVVARKYFVVGARRG"
     gene            complement(15056..15598)
                     /locus_tag="VN178_05235"
     CDS             complement(15056..15598)
                     /locus_tag="VN178_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWS80408.1"
                     /translation="MGRFAALFYGSLALVAALWCGLRGFDVRLVGESLGASVLLGVVT
                     AACTVSLGLLAYRFLPALREISEELAPRLVDGAASTSLVLVAIFSGVGEEAFFRGALQ
                     QEFGLVIASVLFGLAHIGPDRRYLVWTAWAVLAGFVFGLLYEFSGGLLAPILAHSGHN
                     AATLLLWKRSRRGQKKAGGP"
     gene            15730..>16737
                     /locus_tag="VN178_05240"
     CDS             15730..>16737
                     /locus_tag="VN178_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWS80409.1"
                     /translation="MLDESIFKAYDIRGVYPESLDENVAEDIGRAFVKHLDLAGSRVI
                     VARDMRISGEALENAFIQGVTQAGADVLDLGLVSTDALYFAVGHLEEPGGAMVTASHN
                     PKDYNGFKLCREEAIALSGDAGLGQILDLIVSGDLPGPARYPGSVEETDITEDYAKHC
                     LGFIQTEGLRPLKIVIDAGNGMAGKMVPPIFEHLPFEYVPMYFELDGSFPNHPPNPIE
                     PENVRELQERVVAEGADFGVSFDGDADRCFIIDEKGTTISGDLAAALIAKNVLEKEPG
                     ATILYSAVCSKAFPELVEREGGKAIRTKAGHSIIKPQMRKHDAAFGGEHSAHFYFRDN
                     YF"
BASE COUNT         3064 a         5400 c         5179 g         3094 t
ORIGIN      
        1 agcgaacccg tactgaagat gacggggccc gccatcagca ctccccgcgc cggatctcca
       61 ggaacctctc cagcgtcgag ggagagtggt tctggtagtg gttgttggcg taggcgaaga
      121 ccgtcctgcc ctctccgaga aagccattca cgagctccga ccaccacgat aggtcatcgt
      181 ctcggttctt cttgagatga gtgtgtccct cagggaactc gcggcggttg ccgagccagc
      241 ggatgtagga gaaaaccgcc gtcgccctct ccatccgggg catccccggg tagtcgatga
      301 gcgtcaaggc cacgccgcgc tcgctgagca tctcgggtag atccgagccg atccagctcc
      361 tgtggcgcac ctccacggcg aatcgaaatc cctcgggcaa gcgtctcaag taatcttcga
      421 ggacgcccat cccttcgacg tcgaaggaag gcggaagctg caggagaagc ggtccgagcc
      481 tgtctccgag ctcggccatc gtgcgtagaa agccgtctgt ctctgctgtg gcgccgacca
      541 gattccggtc gtgcgtgatc tccttcggaa acttggcggc gaacagaaac ccatcgggcg
      601 ctacctcgcg ccagttctgt acggtcgagc ggcgcggggt gccgtagaag gtcgagtcta
      661 tctccacggt cgcgtagtgc ttcgcgtact cggctagcct cgaagcggaa gggagggcct
      721 cgggatagag cgttccttcc cagtccgcat atgaccagcc ggaggttccc aggtagaggc
      781 cctcctccgg cggctcgatg ctgccgggag tctcagcgaa gagccgctgt tgcgcagaat
      841 cgtccatcag ccctcgaagc tgcgatgcta ccataccgcg gtctccagga agtccacttc
      901 ctggagaccg cgccttcagc gattagcttt cagctatcag ccaaaatcaa gaagctgatg
      961 gctgggggct gaaagctgta gcgtgctgta tgaacgctct tcctgtacgc tcccgtcgca
     1021 tgtactacat tggtgttatg gacgagagat gccagacaca atgaaggtct tctacagcga
     1081 ccacttcgta ctgctcctgc ccgagggcca ccggttcccg atggtcaagt actcgaagct
     1141 gcgcgagagg gtcgagcgag atgggatctg cggcccgggc gcgctctcga cgccacgcgc
     1201 ggtaacggac gaggagatcc tgcgcgccca cgcccccgac tacctgaagc gggtcgtctc
     1261 gggaacgctc acacagaagg agatgcggcg catcggtttc ccgtggtcgg agaggatggt
     1321 agaacgctcc cgccgcgcct ctggcggtac gctctcagcc tgcctcgccg cactcgatga
     1381 aggcttcgcc gccaatctgg ccggggggac gcatcatgcc ttctccaaca ggggcgaggg
     1441 atactgtgtc ttcaacgact cggccatcgc cgcccgcgcc gtacaggccg caggtagcgt
     1501 gcggcgcgtg gttatcatcg atacggatgt gcaccagggg aacgggacgg ccgcgatcct
     1561 ggccggagac cccacagtgt ttacgttctc gatccatggt gcgaagaact tccccttcaa
     1621 caaagaggtg agtgatctcg acgcgccgct acccgacgga gccgacgact cggagttcct
     1681 ggggacgctc gaaagaggcc tgcgagtggc gctcgacgcc gcagacgcag acctcgccat
     1741 ctacctcgca ggtgccgatc ctttcgaggg agatcgactc ggcaggctct ccgtaacgaa
     1801 gtcagggctc gccgagcgag accgtatcgt tctcgaaacc tgcagggaac gcgaaatccc
     1861 cgttgcggtt acgatggcgg gaggctatgc caacgaggta gaggacacgg tggacataca
     1921 cttccaaagc atccgtaggg cggccgacct gctggagagt aaaggggata gatagtgaca
     1981 cgccaaaagt gagcgaagac cagacgtcct gcgtcatcgt aggggccggc atctccggtc
     2041 tgctcgcagc gaaaacactg aacgaagaag gttggaccgt tacggtcctg gaaaagagcc
     2101 gtggcgtcgg cggtaggatg gctaccaggc gtatcggcag tggcaaattc gatcacggcg
     2161 cccagttctt caccgtcagg ggcgaacgct tcgccgggct cgtcgaagac tggctcagcg
     2221 caggcgtcgc cacagaatgg acgcgcggct tcgccgaccc ggagggtagg ccgaacgaag
     2281 acggttatcc ccgctacagg ggaaccgagg gtatgacctc catcccgaaa tacatctccc
     2341 gcggcctcga cgtacgcacc ggagagaggg gcgtaagggt tgatgctgcc gacgatggtt
     2401 gggaggtcgt gtgcgaatcc ggatcgaggg ttgcagggaa tgccctcgta ctcacggctc
     2461 cggttcctca ggcattggat ctcgcctcat cgggaagcta cattccgcct gatggcgcca
     2521 ggcgccaact cgaagcagtc tcctacgacc catgcctcgc cctcatggtc ttgctcgacg
     2581 gttcgaccgg tatcccggag cccggaggaa tgcagatcaa gggcgaacct atagactgga
     2641 tcggcgacaa ccggcgcaag ggtatctccg aagcgccggc cgtcactgtc cacgctggcc
     2701 ctgaatggag ccgctcgcac ttcgagaacg acgagacgga gattacagtg tccatgatct
     2761 cactcgctgg caaacgactc gacaccgacc tcgtaccctc cgtcgtcgtc acttccctcg
     2821 ccaggtggcg ctacagctgg gtcacgaacc cccaccccga tccgtgcctc ctcgcctccg
     2881 atgacccgcc actgttgttc tgcggcgacg ccttcgggca acccaaggtc gaaggcgcag
     2941 ccctctcagg tctcgccgcc gcagaccatc tcctcggccg ctaaagcgat ttgcagaggg
     3001 tcatcgaccc ttcccgaaaa ccccgtgcgc acggatgcat ctggcgtcgc gtaacaaaaa
     3061 gtggtacata tgaactagaa aacaacacag tgtgaaggag aaaggggcct tgggaaatag
     3121 aagggtggcg ctaaagccgc acgcgtccaa gatccgtcag tgggtcgaag agggccgggg
     3181 cgacacttgg atcgcgcagg aactgaacac cacgccctcc agcgtacaga gcttccggtc
     3241 gcgaaactcg atctaccgca gggaccccgt caggcgcggc cagctttcgg agcatgctgc
     3301 ggtgctcgag gagacggaag atggcatagt cctgaagacc gacgccagag actcggaggt
     3361 cttctcccgt gagtggcgcc actacgtgca tgggtcgcca gaagacctgc aagtggtgat
     3421 cacgcaggac aggatctacc tggagaaagt ccgctagctt gccaggagag cccgccgtcg
     3481 ggttgcggct ccctctggag gatgcagaag cgctgcacgc gctcctcgag cgtcttctcg
     3541 aaagcgggca acaggatcca cgcctcgagc actcctacag gctcctgggc tggcgcatac
     3601 tcgccgcaaa gggcggtagg ggcctcacag gacgcatggc gaacctggca cggcaagcgg
     3661 acagcctcga agaatacgaa gcagccctcg aaagggagct cggccccgtg cttgacggcc
     3721 tggagcgcgg cgagaaccgg gacccatgaa tagagttcgt tatcaaactg ttgccttagg
     3781 gtagcgttac tgtaacctcc gtacactatt ttgttggcac tgggctcttt cccccctttc
     3841 cggcccagaa gagcaggccg ggccgcggat cacgcgcggt ccggcatttt ttgcgtgtgt
     3901 taacctctgc tggcgttatg cagtaccttc tcagttcgct tgcgacgatg gtcttcatcg
     3961 gcatactcgc gctcctctct caatggtcga ggaagagcag gagggccgag atatcgctgt
     4021 tgatcgtgct ggtgtttctc tcgctcctgc tcttcgccac cggtgccttg ctcggcataa
     4081 tatgggtctc gggccagatg cccccggatg cctatccgca accgttgttg gcggtcacgg
     4141 cggtacccgt tacgctgtcg ggtttgatcg ggcttgcact ctgcgtccca acgctgctga
     4201 agatcgttcg cggcaggccg aacggggagt tctggacgga cccgccgatc tttctcgccc
     4261 tctggctgtt cgccactgtg ttgcttgcca acaatgtggt aggcgtgctc gggttcgagc
     4321 agttggatca ggtcgacgcc ttctctttgg ggaccggagg caggatccca ccggtcgcca
     4381 tactggcgag ccagttaccc ttcgtcgtca tcgcgcttct gggggtcggc gccggcatcc
     4441 gccgcggacc gcgcgagacg ctcgcccgac tcggctacgg ccccatctcc ctcaaacaaa
     4501 taggtatcgt cgtgctgttt atcgcgggcg cgttcgcgct ctcggtggct tccgacgccc
     4561 tgttctcgca gttgcagccc gatctctatc gcagggtcgg tgaggtctct cagaggctct
     4621 tcgacccgaa aggtttgagc ctggccgcgg cggtgctctt cgccttgctg atcggggttg
     4681 gcgcaggact cggagaggag acgctctttc gcggcgccgt gcagccggtc ttggggatac
     4741 cggtgacttc cgtccttttc gcctccatgc acgtccagta cggtccctcg ctgctgctcg
     4801 gctacatctt cgtgctctct atcgggctcg gtctcctccg ccgccatgtc aacaccacgg
     4861 cgagtttcct ggcgcatgct ggctacaaca ccctcggcat cctggccgta tacttcttcg
     4921 gctggtagag aacagcggtt gggcctaagc ccagcgtctc atcagcagcc attccctctc
     4981 ggcgccccgc gtatcagagc cgaatctctc tacggtctcg aatccggccc ttacatagac
     5041 gctgatggcg cggtggttga aggcgaccac ggtgaggcgg aacgccgggg gtgagtaggt
     5101 ctcccgggcg aagcggaggc cggcgtgtac gaacccttct ccgaggccct acccgtgaga
     5161 tccggtctca tacctagccc cacatcgagc gcgggctcgg tctctacgag gccgagccgt
     5221 tttccggagg cgacctgcgc gtcctcgccg aaacagaagt atcccgccag atctccgctt
     5281 tcgtcgtgaa ccgagtggta ggacgagcgt ggttgcagga tcgcatccac ggaggtggga
     5341 tccccgtcgt agatcgagta gggttcttcg tagcgccagc acgagatggt tcgtgcgtcg
     5401 ctgggcgtga tctgcttcaa ggcgaagcgc aacgtcggtt tcttgggagt ctaggccgcc
     5461 agagagtcgc tgacgggaac taccggcgcg ggttcgagcg ccgaagcgat acggacgagc
     5521 agctcgctga cctcgacgtc caatccactg gcgatgctct ccagtaccct cgaagagggg
     5581 tccttctcgc cgcgttctat ctcggcgagg tacgagaccg agacatgcgc tccttcggcg
     5641 acctgcttta gagtcagccc ccgctcgttg cgttccgccc gcagggtctc gccgatcgtc
     5701 gagagaaggt ctcccgcgtg tcgctccgga ctcatctcca aggtcttcac gcccttgatt
     5761 ctatcttcca accggcgctt tgcgcaaccc gcccgccaac ttcttgacca ggagcacctc
     5821 ttcggagtca gcctcggggg tcccgtaggg cgtctccatg accacgggca cgcccggcag
     5881 accgtcgaaa aaccccggcc aggcgtcgag tggtatgcgt ccctcaccca ccttgcgatg
     5941 cccgtcgcgg tggctaccga gttcgttctt ggcgtcgttt aggtgaacca gcgcgaccga
     6001 gtctcccagg gcgtccacga gctcctccgc gacgtctcgc cctccactct ccgccgacag
     6061 gtcgtagccg gcaacgaaag cgtgcgccgt gtccacacag accctcaccc cgccgcgccg
     6121 cgcgacctcc cggagttctt cgaacgtaga gcagagctgg gtaccggccc cgacggaatt
     6181 ttcgaccacg ggttcggcca cgactcctgc gcctccgtcg agacttgccg cccgttccct
     6241 cgcgcgcccc aggccatcca cgagccgctc catgcccttc tcctcgccgg ctcccccgtg
     6301 gctgccagca tgcacgacga ccaggtcgac acccagcgcg cccgcggcgg acagctcgct
     6361 ggccagggcc cgcaccgagt attcccgctg ctcctcactc gtggaggcaa ggtttatcag
     6421 gtacttcgcg tgtgcgacga ccggcgagat acccatctcc aggcttcctt cgacgaaggt
     6481 ttccgcgtcc tggcgtcgcc tggggtcggc ccaggcctgc gggttggaga cgaagatctg
     6541 gatggtctcg cacccgagct tgcgcgcggc ccaaagggtc ttggcgagtc cgccggaggt
     6601 cggaaggtgt cgcccgattc tgttggtcct ctctggcatg gcggggattc taccgccctg
     6661 cggcggtact caccaggaga gatataatct cccgagacca tcgagatgat cggaaggagc
     6721 caaccgcatg gcggtcgaag gcaaggtagc gaagattctg ggaaacaacg agatcgtcat
     6781 aaaccgcgga aggggcgagg gcgtccggca gggcatgctc ttcgagatat tcgcacccga
     6841 gggcgaggag gtctgggacc ctgatacggg cgagacgctc ggcaccgtcg aggacgtaaa
     6901 ggcgaaggcc gaggttacgg aggtcaagga caggctcgcc gtcgcccgtc tgcagaacgc
     6961 gcaggatacg ccgttcgggg ccgtcaacat cggggagatg caggagaacc tgcagcgtat
     7021 gttcgggcag atgttcggcg aaggcgtcca gatccgcggc ttcggcgcta cctctggcga
     7081 cgaccccgac ctcgagtcga tgctcggcgg ccccctgcag gacctctcac aggtacaggt
     7141 cggggacgct gtgcgcgaga tcaaacgccg ctagagcgga cgcaagtacc cccacattga
     7201 acaccctctc ccgcctaccg gtgactttcg gcctcatagc cgcctgtgtg accgtgtacg
     7261 ccgccgtcgc gatcgcgggc cagggctacg gttacccgct taacgtcggg ctggttacac
     7321 agccccccga ggtgctcgcg cggggcgccc tcgtgcccgc cgccgtcgcc ggtggccagg
     7381 tgtggctctt gctgtcgagc atgttcctgc actcgggatt cattcacctg gcgctgaaca
     7441 tgctctctct atactttctc ggctcgttcg tcgaacaagc attcggaaag agccgtttcc
     7501 tcgcgctcta cctgtgcagt gggctcgccg ggggcatcgc ctacctctac ttcggcgcct
     7561 tcgaggtgcc cgccgtaggg gcttccggcg cgatcttcgg tctcctcggg ggcgtgttcg
     7621 gttactccgt gcgccgcggc accttttcct ggcagaatcc cctgatccgc cagctcctga
     7681 tcctgaccgc cctgaacctc tacatcggat tctccgtacc caatatcagc aacacggcgc
     7741 atatcggcgg cctcctcggt ggggccgcat tcggatggct cacagctccc accgtctact
     7801 ccggcaaacc actccgcgcc gcgacaccga ccgtcatcct gttcggcgcg gagatagtct
     7861 tgttggccgt ttggttgctc gtcttcgcgt aaaatgcggc aatccgcagg atcgaggacg
     7921 gttcacgaac cgcccactct acccgtgctg aattgctatc aatcctacgg agacgacgat
     7981 gagcgtcacc acgaacacga taccggccaa cctgttcgta tcctcggcct gggcctcggg
     8041 gaggaggtgt gcggcgccga tgtatgcgag caccccgccc gcaaccccgc tcagaagacc
     8101 gagagcgcct tcccctggcg cggggaaggc caccgtcaac gccgcggcgg ccgggatcgc
     8161 cagcccgagt aagaccgcca ctctcatgac ccccgccctt ccgacgcgag ccgcgatcag
     8221 gacggcggcc agggagatcc cgacgggtat ctggtgaacc agaaccccga gccctacgag
     8281 ccaggaggtg acggccccgg ccttggcgcc tacgcccaga acgaatccgt cggcgacgtt
     8341 gtggacggcc aaccccagga cgaaaggccc gagcgcgtgt ttgtggacgt gctcgtcggg
     8401 caggtggtgc gtgtgggccc tggtgaacgc ctcggtcacg aacaggaggg cgaaccctcc
     8461 gacgaacccg gcgaccgcgg cctctccacc gagttccagg ctctcgggga agaggtcgac
     8521 gaacgcgagc gcgagtagta tccccgcggc gaaagcggcg gcgtaagacc ggcccctgac
     8581 ccccaactct cccccgagta gggcgagaag actcgccgcc gaaaagccca ccgcggccag
     8641 cgaagccatc aagatcgcat tcacagccgc caccctatca caagatggca gaggggatct
     8701 cgcgtgtcaa aacggtccta tgggagaaat tgcgggatgg ccgtcacgtt ctgcgaaatc
     8761 tttgtaaact ctctcgaact cgtggcttca ggacatcaca aacagctgac gctatttgcc
     8821 cgcttccgct cgattctgag cggtcgcgac tgggtctacc tttccgggct tctggttccc
     8881 ttcgtggtct acaatctggt gttgaagggg ctccggatag attctcaaga ggaacttccc
     8941 gggggattcg ccgccttcgc tttgatccgt tcggatttgc tctttaacct ggggtatgtg
     9001 tttctctggg tagggctatt cgccctgacg cggcggagtc gtctccgctg gcctgtggtg
     9061 gtcctcttcc atacggtcac gatagccgtc attgcgacga cgacctcggc ccacgggtac
     9121 tttcaggaga ccggctcgac gctcagcctc agcgtcatca cgtactcgct cggctcgctg
     9181 ggcgagatca cagacgtcat cggcagcgtg acttcgctgg ctatctgggc gtgggttctg
     9241 gtcatcctcg gctatatagt gcttggtccc tggataatag cgacgctcgt ctgtggaaag
     9301 cctggggagc ctgattcctt cggttctccg tccacatcca ggctgttcgc cctcggggca
     9361 ttcactatgt cgatagggtt catctttctc tcgctgccgg tcggtgccgg ggtggccgga
     9421 aaagcgttct ccagggacgc cgtggtcaat atcgtcatgt cggaggtgga gcacgcgcgg
     9481 atcagagccc tcgtctcgaa cgctgcttcg atggagcctc ccgtggatac ccgcctgcaa
     9541 aagacctccg agaccgagaa gaagaacgtc gtcctgatcc acctcgagtc tgccagggca
     9601 cgctccatga cgccctacaa tgaggacctg gacacgacgc cctacctcga cacgctggcc
     9661 aaaaaaagcc ttctggccga gcgtgcctac acgatcgtcc cccacacctc caaggccgtt
     9721 acctctgtaa actgtggcat agatgcgcac ctggtgcgcg agatcaccga ggtcgagccc
     9781 ggcggcgtgc cggcccggtg cctgcccgaa ctcttgaagg agcagggata taattccgtg
     9841 tggttctcct cggcgaccga gcacttcgaa gaccgcaggg acctcgtcga gaacttcggc
     9901 tacgaggact tcctacccgt cgagacgatg gagacggcgg gcttccagaa gtccaactac
     9961 ttcggctatg aggacaacat catgctccgg ccgagcagag agtggttgga gcagcacaat
    10021 gacgaaccct tcgttgccac ctacctcggg gtcacgggac accacgacta ccggcccata
    10081 gaccgctacg ggttgaagga ttactcgaac ctccccgggc tcgaccacta ccagaacgag
    10141 atgcgttacc tcgacttctt cgtgaggaac gtgatgaatc agtacaagga gctggggctg
    10201 tacgacgata cgatcttcgt gatctacggc gaccacgggg agggattcgg ggagcacgac
    10261 ctctaccagc acgacaacac catctaccag gaggggctca aggtaccctt catcatccac
    10321 gctcctggac gctttgcggg aggcgaacgc gtcgagaccc tctcgaacca gctagacgtc
    10381 ctccccactc tgttcgacat gctcggctac aaggtcacag gcggccaata cccgggtaga
    10441 tccctccttg gtgcgccaga ggggaaccgc acgctgtact tttcttgctt cgacgagtac
    10501 aggtgcctcg ccagcatcaa gggcacgaag aagtacatct acttcttcgg caaccagcct
    10561 gaggaggtct acgatctggc gtcagatccc ctggagagga agaacatcgt gggtgagctg
    10621 agccgtaagg agttgaaaga caaacgtctg caggtgctcg cgtggtactc gaaggtcaac
    10681 gccatgtacg agaagcgaga ggccaagcaa gagtagtagt ccctacctag cttgactcgg
    10741 gacgtttgcg cctggaaccc gagtgcctca ggagcaggat catggcgaag cccaaagcca
    10801 ggaggctgct aacgaagagg ttgccgcgga tggtcccgcc gatgaggaaa gccgggtcga
    10861 cgcggagggc ctccacaaag aagcggccga atgagtagag gctcacgtag agcaagaaga
    10921 cgtcgccgtt tttcagtcgg tcagcgaaac ggcgggccac ccacaagagt atcaggcaga
    10981 cgacgacgtc ccagatcgac tcgtagagga aggtcgggtg gaaggccgcg gcgtctgagt
    11041 atccgacggg tctgttctcc ggcgctatcc tgatggccca gggtagatcc gaaggccgtc
    11101 cgaagagttc ctggttgaag tagttgcccc agcgcccgat cgcctgggcg aggaccaatc
    11161 cgggtgcgac ggcgtcggcg aacgcccacg ggcttatgcc cctgatccgg gagaagatca
    11221 agagccccac gaaccctccg acgacgccgc cgtagatccc caggcccccg ttccagacct
    11281 cgaagacgcc ggggaaaggg tcgcccgcgt agagcccgta gtcggtaagg acgtgatagg
    11341 ccctggcccc gataaagccg aggggcacga taaagaacag cgagtcgagg gcaagggcgc
    11401 tgtcgtagcc cttgcgttcc agctccctcc ccgtgagcca ggtcgcgacg atgataccga
    11461 gggctataca cagcccgtag tagcggaggg cgaagggccc tacctcgaag attaaggggg
    11521 aaggcgggct cggcagcgag agaaggttca cgctactcta ccggggccga ttcgctctcc
    11581 gtggaagcgt ggtcccgcgg ggggctgtcc gtccggtagg ggtccttggg cgactgctcg
    11641 acgccgtagt aggcttcgag tatgtgtcgc acgaccggcc cggccgtcat ctccgagccc
    11701 tcctcgaaag taccgccgcc ctcgatcatc gtgacgacga ccagcggctg ctcctctcga
    11761 ccctcgaccc agccggcgaa ccagttcacg tagtccttcc cggccggcag ctcgcccgtg
    11821 ccgctcttcc cggcgaacgg gagggtcgag tccacgaaga ccggtcccgc ggtgccgtcc
    11881 tcctccgtga cgcccctaag gccccgtatc acgaggtcga tctggtactg gttcgtgtct
    11941 acccgtccgg cgggcttggg ggagaacgtc tccaccacgt tcccgttctg gtcggtgatc
    12001 tccttgccga cgtgcggggt gacgagcgtg ccgccgttgt agagggcgga gtatgcccgg
    12061 gccatctgga tcggggagac gagcaagtcg ccctggccga tggcgaggtt cacccagtcc
    12121 cccacgctcc agtaggcccc ttctgtcgaa ccgtgcaacg ccttgtgcag ttccctctct
    12181 ccctgctcgg tggggatgcg accttcggtc tcgccgctga tgtcgatgcc ggtgagccta
    12241 ccgaaccccc accgctcgta gtagtgtggt agccagtcct tgtccctagt ctggttccag
    12301 atccagtcgg cgacctggta gaagaagata tcgttggagt cggcgatggc ctcggagatg
    12361 ttctgggggc catggtcgcc gaggaagtgg tagttggggc tgttctgccg ccagctcctg
    12421 tagcacgggc tcgccgagac gctcgacggc atccagcagc ccgtcccgct catggcggcc
    12481 gtatcgttga cgatcgtgta ggcatctatg acgccggcgg ccaggccggc catccccgtg
    12541 aagatcttga acgtcgaggc gcccggctgc ccgccgacga tcgcgcggtt gaggaacgga
    12601 tcgttcgacc ggtcggacgc gaggtagttg taggcggcga gttccctgtc gccggagatg
    12661 cccccgacga acagctgcgg gtcgaagtcg ggacgactgg ccatcgccag gatctccccg
    12721 ttctcggggt tcatggctac gacagccccg cccctgccgt cgtagcccgc gtcccaggtg
    12781 cgctccaggg ccgcgtcgag ctctttctcg acgaccctct gcaagtcgat gtcgatagtc
    12841 agggtgaggt tgttgccagg ttcgggctcc gtgacgtggt cgggtaactc caggcttggg
    12901 ttcacgttta tggggttacc gttttcgtcc acaaacctgc ccatggaatc gaccctgctc
    12961 ccttcgggca ctatgcgccc cagggcgtct acgttgtagg ttctctgccc tgctttgccg
    13021 cgcagcgcct cctcgtagga gagctcgaca ccactcttgc cgacgatgga atcggcgggc
    13081 aggcctttga aaggttcctg cttgagctca tcgtggttga cggcgccggt gtacccgagg
    13141 atgtgcgcgg ccagcctgcc ctcggggtaa ctgcggaccc agtcgtcgtt cacggcgacc
    13201 cccgtgaact ccccggcgcg ctcgctcacg tacgtcaccg cgtcgcggcc cgcgttctcc
    13261 tttacgagga tgggactgta ctggcttccg gtctccatcg ccgcgtcgta gcggcccaag
    13321 accgcctccg tgtcggcgcc gacgaccgtc gccagttccc gcactttgtc cctggagatt
    13381 tcatccggta tgacggtaac gttcagcccc gggacgttgt tggcgaggat cttgccgtcg
    13441 cggtcgtaga ccacaccccg ctgggcgggg atcttgatgt tggtgtgttg cgtcgccctc
    13501 gcggcgaggg tgtagtcctg gcccgtgagg acctgcaggt accacaacct ggcgccaagg
    13561 atgaggaaga ccgcggcgat cagagccgcc agagtcacta cccgcacctg catgttgccc
    13621 ttggtcatcg gcggagactc cacacgcttt ttcgcggagt tcgcctgctt cttctctctc
    13681 accggcaacc ggagcaacct ctacccccct tacgcgacga gcactaccgc tcggcgacct
    13741 tttcggcctg gacgatcaaa cgcctggagt aatccggaag cgtacacgtc tgtagcgaga
    13801 ctatgttcct gccctttagc ggcctcgtca ccgacacgtc atcggggtcg acgacaaagg
    13861 tcctgaacac cttgtagacg taacgcttgc ccgccgcgtc gatgataaag accttgtccc
    13921 cattctcgag cgtgttcaaa tcccagaacg acagccagct gtcggttccg gggaaaccga
    13981 gccggtggcc ggcgatgtag acgttcgcct gcctgtccca agggttgccc gtcccgcgga
    14041 ggtgcacggc ggcgtgtttg cggaacgcct tcttgtcgtc gcttacggtg tagggtacgg
    14101 tgtcgttgcg tatctgggcc aactcgggga tcgtcatccg cagcatcctg ctctccggcc
    14161 cctggtcgac ggctctcttc ctggccgccg tcccgtctgg gtccttcctc tgcgtctccc
    14221 ttttctgcct ctcgatggcc ttgtcgatct tgcctgcggc cacggggagt ctgggctgtc
    14281 gggaagccgt gctgaccttc tcggcgaccg cgacttcggc ctcggcgtcg tgttgcgggg
    14341 atccgacgaa gaagctcgcg gtcaccacta ctcccacgcc gacgaccagt aggctcaaca
    14401 cgttgatggc cgtttcggtg atgcgcgcta tggttccctc cctacgcggt cgcgcggacc
    14461 gacgtctcgg tcgcaatcaa ttgtatccca aaagcggacg actattagcc gcgccgggcg
    14521 ccgaccacga agtattttct ggccaccacg ctattcccgc cgttctcccc tgatacggtg
    14581 tagaccgcca ccgtgtaatc gccggaaggc agccgccgtc ccgatccaga gcggacggcg
    14641 aactttacca cgccggaggc cctgccgtat gtcacgctga gtcgctcctc gccttctttg
    14701 gagacaacga gctcgcctcc gccgccgaat agacggccaa gggcggagac cctcgacgtc
    14761 cgcccgactc tcgcctcgag gtcctcgtag gcggcgaagt cctccaccct aagatagacg
    14821 taaacttcct cggccccccc gccgaaacgc agcctgtccg aggtcgggta cactctgtcc
    14881 tcctggctca ccgcgatgtc cctggccccg ggtgagatca actcttctcc cggcgagaag
    14941 ttcaccggcc agagggatac gactgcagcg agcaacagga tcccgaggca gactgcggcc
    15001 agcgcaggac cgacacggtc tcctttcttg gccgtggttg tgcttgggag cttgttcagg
    15061 gaccaccggc cttcttctgg cccctacggg agcgtttcca gagcaggagc gtcgctgcat
    15121 tgtgcccgct gtgggcgagg atgggggcga gcagaccgcc actgaactcg tagagaagac
    15181 cgaaaacgaa gccggccagc acggcccacg ccgtccatac gagataacgc cggtcggggc
    15241 cgatgtgggc caggccgaac aacacggagg cgatcaccag gccgaactcc tgctgcagcg
    15301 cgccccggaa aaacgcttcc tcacccaccc cggagaagat cgcgaccagc acgaggctcg
    15361 tcgaggcggc gccgtcgacc aggcgcgggg ctagctcttc ggagatctcc cgcaaggcgg
    15421 gtagaaagcg gtaggcaagg aggcccaggg agaccgtgca ggcggccgta acgacgccga
    15481 gtagcacgct tgcccccaaa ctctcgccga caagccgcac gtcgaagccc ctgaggccgc
    15541 accacaaggc ggcgacgagg gcgagagaac cgtaaaacag ggctgcgaac ctgcccaacg
    15601 cgcgcctaat ccgggtaggc tgtgaaccgg tctgcaggat ggaccataac ccgttattgt
    15661 accggacgga gaaattcaca ggtcaaacca caggtctcac acagaccggg aggagtccgg
    15721 aggtaggaga tgctcgacga gtcgatattc aaggcctacg acatccgtgg cgtctatccc
    15781 gagagcctgg atgagaacgt ggcggaagac atcggccgtg ccttcgtaaa gcacctggac
    15841 ctcgctggct ccagggtcat cgtggcccgc gacatgcgca tctcgggcga ggcgctggag
    15901 aacgccttta tacagggggt tacccaggct ggcgcggacg tcctcgacct cgggctggtc
    15961 tcgaccgatg cgctctactt cgccgtaggg cacctcgaag agcccggcgg ggccatggta
    16021 acggcctcgc acaaccccaa agactacaac ggcttcaagc tctgcaggga ggaagcgatc
    16081 gccctctcgg gggacgcggg gctaggccag atcctcgacc tcatcgtctc cggggatctg
    16141 ccaggccccg cccgataccc cggttccgtg gaggagaccg atatcacgga agactacgcg
    16201 aaacattgcc tgggctttat acagacggag gggctgaggc cgctgaagat cgtgatcgac
    16261 gcgggaaacg gcatggcagg caagatggtc ccacccatct tcgagcacct ccctttcgag
    16321 tacgtgccga tgtacttcga gctcgacggc tccttcccca accatccacc gaacccgatc
    16381 gaacccgaga acgtcaggga actccaggag cgcgtggtcg cggagggcgc cgacttcggg
    16441 gtatccttcg acggcgacgc cgaccggtgc ttcattatcg atgagaaggg cacgaccatc
    16501 tcgggcgacc tggccgccgc cctcatagcg aagaacgtgc tggagaagga gccgggggcg
    16561 accatactct acagcgcggt ctgctcgaaa gccttccccg aactagtcga gcgcgaagga
    16621 gggaaggcca taaggaccaa ggcgggccat tccatcatca agccgcagat gcgaaagcac
    16681 gacgccgctt tcggcgggga gcactcggcc cacttctact tcagggacaa ctacttc
//
LOCUS       DATMVF010000108         8160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_384856, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000108 DATMVF010000000
VERSION     DATMVF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384856"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VN178_05245"
     CDS             complement(<1..946)
                     /locus_tag="VN178_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS80410.1"
                     /translation="MAEQFDLVVIGGGNAGYIPAIRASQLGMSVALIEKREGGHLGGT
                     CLNVGCIPTKALLQTAHLLHDARNGEDFGVKVGEVEFDYPQAAKRREQVVNQLRRGVQ
                     GLMKKNKVTVYNGVGSFVEPKKIKVETEDGDEELEAGNVLISTGSAVNTLPGLEFDGE
                     KIISSDDIVTNDEDYPESVVILGSGAVGVEFASMYSDFGAEVTIVEILDRLVPLEDPE
                     ISEALRKEFEGRGITVLTSTKADPESLEKTDSGIKITVADAESGEQEETEGEGGGYGG
                     EDPPTGEASDADGDTLEAEALLVAVGRKTVTEDLNLDAT"
     gene            complement(1075..1932)
                     /gene="mutM"
                     /locus_tag="VN178_05250"
     CDS             complement(1075..1932)
                     /gene="mutM"
                     /locus_tag="VN178_05250"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWS80411.1"
                     /translation="MPELPEVETIKEDLRELVVGSEIERAEVLAPSLVEQPSAEAFAR
                     RLEGVSITGVRRRAKHLIVELGSGDSLVFQLKIGGQLLLVPPVEEPETALMLVLDLDG
                     DRRLFLRDETGFTRARLLDSDELEERLASLGPEPLEEEFDTGYLQRTVGSRRAQIKPL
                     LLDQKVVSGIGNIYADEILYDARLHPRRKANTLSADEWEALYTAIRSNLAAGIEHRGT
                     TVRLYKDVLDRPGEHQNYLRVFEKHGQPCPGCEGEVVREKVSGRPSHFCPACQQEDGS
                     GEDGQLELQ"
     gene            complement(1976..2626)
                     /locus_tag="VN178_05255"
     CDS             complement(1976..2626)
                     /locus_tag="VN178_05255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS80412.1"
                     /translation="MEILDRNGIFGGLVVDLGCGSGQWARKLVDTGYRVLGIDISEAM
                     IELSRTKVPEAEFRLGSLFEVEIPRCQAVTATSEVLNYLFDAENEERGLGRLFRRVHD
                     ALVPGGVFVFDVLGPGQVPPGATTKGFSVGEDWAVLSELEEDAERGTMERRIVSFRKV
                     GGYYRRDDEVHRVRLYDPAELTAELERAGFRVRKIRSYGDFPLSEGHSAFVARKPV"
     gene            complement(2733..3467)
                     /locus_tag="VN178_05260"
     CDS             complement(2733..3467)
                     /locus_tag="VN178_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWS80413.1"
                     /translation="MNPEEFLQRLKREVIGHPALTHPFLERFGEGDADAEGIRTFAIQ
                     YYRHVRVSRLYLAALISGCRDDEKLQLALAEVLFDEYGHLDPAETHPALYRRFLGALG
                     IGEEEWEEPPTLPEIEMYITTHYALSGHPDFRLGLGALGPASEWPVPPIYARLSDGLR
                     KAAGLPDEALEIFTSHVTMDVTHARIMMDAIAPYAGDEEGQRRVREGAMRSLDARSVM
                     LGGLYRAVYREPVPVFDAPPVRLTGG"
     gene            3723..4253
                     /locus_tag="VN178_05265"
     CDS             3723..4253
                     /locus_tag="VN178_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80414.1"
                     /translation="MTDTRNNFRQDWLSVPRRREGERLDPHVYKRGINGLTVEEALRR
                     AFPRVIDILGRSGEEYSEFELQLVRQTVRACLISQVSRANLYRIAGILVADRRDEGAH
                     IDTTLALAYVAALREAFLTELSVGERVQTPLGSGEVRYVSGDGANAAIELDNDYEIWL
                     PVEKVTRPNPLVRRAS"
     gene            4321..5241
                     /locus_tag="VN178_05270"
     CDS             4321..5241
                     /locus_tag="VN178_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWS80415.1"
                     /translation="MQKAKARVICNPTSGGGAYDPDEIREKLDGLDLDWIETKGPQDA
                     IEAAQDWQEGLLMVAGGDGTINDVVNGLGRAGFPEGVTLGILPAGTGNDLAATLAVPD
                     DPELAEDVIRQNRERRLDVARVRSEGIGERFFINVATGGLGAEISNANNEELKKRWGK
                     LSYLRASLEVARNFEVHELTLYLDGEGHQVEAVNIAVGNCRYTGGGWPATPKANPEDG
                     LLDLVVIETLGAAELFDLAPAVLAESDYLEKEGVQFVRAKEIRVETQPPGLEFTVDGE
                     VIGDEPAQFSVLPQTLKVIVGPDYIPEPAG"
     gene            5251..5493
                     /gene="moaD"
                     /locus_tag="VN178_05275"
     CDS             5251..5493
                     /gene="moaD"
                     /locus_tag="VN178_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HWS80416.1"
                     /translation="MIRVLLFGAAADRAGTRETELPVEEGVTLAELWPLLAGRHPGLV
                     PMRDTLAFAVNGEYARGDAGVSPGDEVAVLPPVSGG"
     gene            5494..5937
                     /locus_tag="VN178_05280"
     CDS             5494..5937
                     /locus_tag="VN178_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWS80417.1"
                     /translation="MFTILEEPLDVGALIQDAQRPDCGAVATFVGTTRIDESEGAAVE
                     YLEYEAYRPMADRKLEEIGAEIEGRWDVGHVSIVHRLGRVDPGEASVAIVVAAPRRGP
                     AFEASRYAIERIKEVVPIWKREVWSDGYVWVGSQVGTRDQVSGFR"
     gene            complement(5994..6368)
                     /locus_tag="VN178_05285"
     CDS             complement(5994..6368)
                     /locus_tag="VN178_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HWS80418.1"
                     /translation="MQPQDERRDAQEQTEIREHLANERTLLSWVRTGVSLISIGLVVE
                     RAGALVDATMKVGSTSASELFGLALALLGALTLVLGTTQFLGNRRRISTGEFVPSAAA
                     YLIIVAGSLAFAGAFVIYVLLT"
     gene            6531..7847
                     /locus_tag="VN178_05290"
     CDS             6531..7847
                     /locus_tag="VN178_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWS80419.1"
                     /translation="MIKGDRLLDRRAGLEQEFFLVENSGHPSERADEFLELCRKESGG
                     RVCFAPEFVLGLVEVNTPPVYTLHDLEREYAQNLRIALRTSRSLGLRLYPLGTYPLPL
                     EPSVRDGLDYRVQVNTVGPERFEDAGRCAGTHLHLELQAGTIDEEAGISAAASSAARQ
                     EVSNIYNLATALDPALVALTRSCPFFEGRRTGLAVRTVHYRGSAIFGWEGVYKDLPQV
                     GALLPYAEDAEHLIRQQFDRYEAWLSAMDRAGVHRRRFAEAGGDLLRPAWNPVRLNRQ
                     GTLELRGMDSNYPEVTLATAAIILRAADRVRQDNLEVLPDDGVLAFEVTGDVLRVPGF
                     GHLGGELLHAAVTTGAGDETVAAYLDSILEFAGVEDERLAILFHHRCATGSYPTTEAG
                     IVEAYDGHLSEDEGLRLVLEACDELETQVSHLSRSQRRYEEPADVS"
     gene            7882..>8160
                     /locus_tag="VN178_05295"
     CDS             7882..>8160
                     /locus_tag="VN178_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWS80420.1"
                     /translation="MGKERAVIGMDFGVRVAAVVEREGELLLVRHQKPDREPYWVLPG
                     GRLEPGETIPECAGRELAEETGLTARFSGVLYVSEFLREGRHTIDVIAR"
BASE COUNT         1447 a         2583 c         2603 g         1527 t
ORIGIN      
        1 tggtcgcgtc gaggttgagg tcctcggtta cggtcttccg cccgacggcg acgagcaacg
       61 cctcggcctc cagggtgtcg ccgtcagcat cgctggcctc gccggtgggc gggtcttctc
      121 cgccgtatcc accgccctcg ccttccgtct cctcctgctc accagattct gcgtccgcca
      181 cggtgatctt gatgccgctg tcggtcttct ccaggctctc gggatctgcc ttggtgctcg
      241 tgaggaccgt gataccgcgg ccctcgaact ccttcctgag cgcttcggag atctccgggt
      301 cctccagcgg cacgaggcgg tccaggatct ccacgatggt cacctcggcg ccgaagtcgc
      361 tgtacatgct cgcaaactcg acgccgacgg cgccagagcc gaggatgaca acagactccg
      421 ggtagtcctc gtcgttggtg acgatgtcgt ctgaggagat gatcttctcg ccgtcgaact
      481 ccaggccggg cagggtgttg acggccgagc cggtggagat caggacgttc cccgcttcga
      541 gttcctcatc gccgtcttcg gtctctacct ttatcttctt gggttcgacg aagctgccta
      601 cgccgttgta gacggtgacc ttgttcttct tcatcaggcc ctgtacgccg cgcctcagct
      661 ggttgactac ctgttcgcgg cgcttggcag cctgcgggta gtcgaactct acttcgccga
      721 ccttgacccc gaagtcttct ccgtttctgg cgtcgtgcaa gagatgggcc gtctggagca
      781 atgctttggt cgggatgcac cccacgttca ggcaagtgcc tccgagatgc ccgccctccc
      841 gcttctcgat gagggccacg ctcatcccca gctggctggc ccggatcgcc ggtatgtatc
      901 ccgcgtttcc gccaccgatt acaactaggt cgaactgctc agccaccctt ggctcctttc
      961 tgttgtaggt ttcaggcatc aggcatcagg tttcaggaca ttctggcgcc gcgccaccac
     1021 gcctgccaga cgataactcc ttcctgacac ctgtagcctg aagcctgatg cctttcattg
     1081 caattctaac tgcccatcct cgcccgaacc atcctcctgc tggcaggcgg gacagaagtg
     1141 cgacggccgc ccgcttacct tttccctaac tacctctcct tcacaccctg ggcagggctg
     1201 tccatgcttc tcgaagaccc gcaagtagtt ctgatgctca cctggccggt ccaagacgtc
     1261 cttgtagagc cgcaccgtgg tccctcgatg ctcgatgccg gccgccaggt tggatcttat
     1321 cgccgtatac agcgcctccc attcgtccgc cgagagcgtg ttcgccttgc gcctcggatg
     1381 cagccttgca tcgtacagta tctcatcggc gtagatgttc cctatgccgg agacgacttt
     1441 ctgatccagg agcaggggtt ttatctgggc cctccgcgaa ccgacggtcc gttgcagata
     1501 tcccgtgtcg aactcttcct ccaagggttc tggtccgagc gaggcgagcc gctcctctag
     1561 ttcgtctgag tcgagcaagc gcgcccgcgt aaagcccgtc tcgtcgcgca ggaagagcct
     1621 cctgtcgccg tcgagatcga ggacgagcat gagcgctgtc tccggctcct cgaccggcgg
     1681 cacgagcagg agctggcccc cgatcttgag ctgaaacacc aacgagtctc cggagccaag
     1741 ctccacgatg aggtgcttgg cccggcgtcg cacccctgtg atgctgacgc cttcgagccg
     1801 ccttgcgaac gcttccgccg aaggctgctc cacgagcgag ggagcgagaa cttcggcccg
     1861 ctctatctct gagccgacga ccagctcccg caggtcctcc tttatggtct cgacctctgg
     1921 cagctcaggc atgcgcgtat ttatacccgc tcggcgccgc cctattcctg ctgcctcata
     1981 ccggtttgcg cgcgacgaag gcggagtggc cctctgacag cgggaagtcg ccataagatc
     2041 ttatcttccg gacccggaag ccagcccgtt cgagctctgc ggtcagctcc gccgggtcgt
     2101 agaggcgcac gcgatgcact tcgtcatccc gcctgtagta ccctcccacc ttccggaagc
     2161 tcacgatgcg gcgctccatc gtaccccgct cagcgtcctc ctcgagctcg gagagtactg
     2221 cccaatcctc tccaacgcta aaacctttcg ttgtggcgcc gggcggcacc tggcccggac
     2281 ccagcacgtc gaagacgaag acgccgccgg ggacgagcgc gtcgtgaacg cgccggaaaa
     2341 ggcggccaag cccccgttcc tcgttctccg cgtcgaaaag gtagttgagg acctcgcttg
     2401 tggccgtgac ggcctgacag cgtgggatct cgacctcgaa cagagatcca agcctgaact
     2461 cggcctccgg aaccttggtg cgcgaaagct ctatcatggc ctcggagatg tcgatcccga
     2521 ggaccctgta tcctgtgtcc acgagcttcc gcgcccactg accgctgccg caaccgaggt
     2581 cgacaacgag gccgccgaat atcccgttgc gatccaggat ctccatgatg cccggcgccg
     2641 ctccgaggac gaactcggca tggccgacgt cgtgtatgta cgcgatgtcc tccccgtacc
     2701 agcgcgtcgt gtcgggacct cccgaggcta tctcagcccc ccgtaagccg gacgggcgga
     2761 gcgtcgaaga cggggacggg ttccctgtag acggccctgt agagaccgcc gagcatgacg
     2821 gagcgggcat cgagcgagcg catggcgccc tcacggactc ttcgttggcc ttcctcgtcc
     2881 cctgcgtagg gggcgatggc atccatcatg atgcgggcat gcgtcacgtc catcgtgacg
     2941 tggctggtga agatctccag cgcctcatcg ggcaagccgg cggccttcct gaggccgtcg
     3001 gagaggcggg cgtagatcgg gggcacgggc cactcgctgg ccggcccgag ggcccctagc
     3061 ccgagcctga aatccggatg gcccgagagc gcgtagtggg tagtaatgta catctctatc
     3121 tcgggcagcg tcggaggctc ctcccactcc tcctcgccga tgccaagcgc ccccaggaaa
     3181 cgcctgtaca gggccggatg cgtctccgcg gggtcgaggt gtccgtactc gtcgaagagg
     3241 acctcggcga gcgcgagctg gagcttctcg tcgtcgcgac acccagagat aagagcggcc
     3301 aggtacaacc ggctcaccct cacgtgcctg tagtactgga tcgcaaaagt ccttatcccc
     3361 tccgcatccg catccccctc cccgaaccgc tccaggaaag ggtgcgttag cgcaggatgc
     3421 ccgatgacct cacgttttag ccgctgaaga aactcctcgg ggttcacctg tcactccttc
     3481 cagagaactc tctacagttt agcgcaacgt ttgagctagt cagcatttca gcacgcttcg
     3541 gcttcgcctc cgcttgtcag cccggcggct gcgtctcgtc agccaatcag caagggctcc
     3601 acgcgaaagc tgaaatgctg aagtgctgac gagcgaagcc cgaagggccg agcgtgctga
     3661 aatgctgatt ttccaggtaa ttaaccattg cctcaccagc ttcagtgaga tataattgcg
     3721 acatgacaga tacgaggaac aacttcaggc aggattggct cagtgtcccg cggcggcggg
     3781 agggagagcg tttggacccg cacgtataca agcgcggcat caacgggttg acggtcgagg
     3841 aggcgttacg tcgggcgttc ccgagggtga tagacatttt ggggaggagt ggcgaggagt
     3901 attcggagtt cgagctgcag ttggtaaggc agactgttcg ggcttgtctg atctcccagg
     3961 tctcccgcgc gaacctgtac cgcatcgccg gcatcctggt cgcggacagg cgcgacgagg
     4021 gggcgcacat cgatactacg ctcgcgctcg cctacgttgc ggcgctcagg gaggcgttcc
     4081 ttacggagct ctccgtcggc gagcgcgtac agacgccgct cggttccggc gaggttcgct
     4141 acgtttcggg tgatggcgca aacgccgcca tcgagctgga caatgactac gagatctggc
     4201 tgccggtcga gaaggtaacg cggcccaatc cgctggttcg gcgcgcttcc taggggcctg
     4261 caattattag gcgctttccg ggtagactag gagggtcgta gagtctacag agggcgcgcc
     4321 atgcagaagg caaaagcacg cgtaatctgc aacccgacct cagggggcgg cgcttacgac
     4381 cccgatgaga tccgtgagaa gctcgacggg ttggatctcg actggatcga gaccaaaggt
     4441 ccccaggacg caatagaagc ggcgcaggat tggcaagaag gcctgctcat ggtcgccggg
     4501 ggggacggca cgatcaacga cgtggtgaac ggccttggca gggccggttt ccccgaaggt
     4561 gtcaccctgg gcatcctgcc cgccgggacc ggaaatgacc tcgccgcgac gcttgccgtc
     4621 ccggatgatc cggagctcgc ggaggacgtc atccggcaga atcgggagcg gcggcttgat
     4681 gtggcaaggg ttcgttcgga aggcattggg gagcgtttct tcatcaatgt ggcgacgggg
     4741 ggccttgggg ctgagatctc caatgctaac aacgaagagt tgaagaagcg gtggggcaaa
     4801 ctctcctacc tgcgggcttc gctggaggtc gcaagaaact tcgaggtgca cgagctcacg
     4861 ctctacctcg atggtgaggg acatcaggtg gaagcggtca acatcgccgt gggtaactgc
     4921 cgctacaccg ggggtggctg gcctgccacc ccgaaggcca acccggaaga cggtctcctc
     4981 gatttggtcg tcatagagac actcggggcg gcggagctgt tcgacctcgc tccggccgtc
     5041 ctggcggagt ccgattacct ggagaaggag ggcgtgcaat tcgtgcgggc gaaggagatc
     5101 cgggtcgaga cccagccccc cggattggag tttaccgtcg acggcgaagt tatcggcgac
     5161 gagccggccc agttctcagt acttccccaa accctcaagg tcatagtcgg gcctgattac
     5221 atccccgagc cggcgggata gaatagggcc atgatcagag tgctactctt cggcgctgcg
     5281 gccgacaggg cagggacccg cgagacggag ttaccggtgg aagaaggcgt aaccctcgcg
     5341 gagctctggc ctctactcgc cgggcggcat cccggcctcg tcccgatgcg cgatacgctg
     5401 gccttcgccg taaacggcga gtatgcgcgt ggggacgcgg gcgtctcgcc cggcgacgag
     5461 gtggcagtac tgccgcccgt ctcgggcggt tgaatgttca ccatcctcga ggagccgctg
     5521 gacgtcgggg ctttgatcca ggacgcccag aggcccgact gcggcgccgt agccacgttc
     5581 gtaggcacga cgaggatcga cgagtctgaa ggcgcggcgg tcgagtacct ggagtacgag
     5641 gcgtaccgtc cgatggccga taggaagctg gaggagatcg gggcggagat agaagggcgc
     5701 tgggatgtcg ggcacgtctc gatagtgcat cgtctgggca gggtagaccc gggcgaggcc
     5761 agcgtcgcca tagtcgtcgc cgcaccgcgc cgcggtccgg cgttcgaggc gagccgctac
     5821 gccatcgagc gcataaaaga ggtcgtgccc atctggaagc gcgaggtctg gtcggacggg
     5881 tacgtgtggg tggggagcca ggtcggaacc agggatcagg tatcgggttt caggtaagag
     5941 atttcgggac ttttcggggg atgggtccgg accagcgggt tccctggcgg gtactaggtt
     6001 agcagcacgt agattacgaa cgctccggcg aaagccagac taccggcgac tattatgagg
     6061 taggccgccg ccgaagggac gaactcgccg gtcgagatcc tgcgccggtt acccaggaac
     6121 tgggtggtac cgaggacgag cgttagcgcc ccgagcaagg cgagcgccag tccgaagagc
     6181 tccgaggcgc ttgtggagcc aaccttcatg gtggcatcta cgagcgcgcc cgcccgctcg
     6241 acgacgagcc ctatcgagat caggctgacc ccggtcctca cccatgagag cagcgtccgc
     6301 tcgttcgcga ggtgctccct gatctcggtc tgctcctgtg cgtcgcgacg ctcgtcctgc
     6361 ggttgcaaga tctactggcc tccttctcgt ccacaccgaa gattacatgc tggagcgccc
     6421 gaaagtttcc tcgccgcccg gatttcggtc ctgggagagc cggtttcccc atccggggta
     6481 gcgtttgttc catagaagat ggagaatacg tcataggact ttgagatagg gtgatcaaag
     6541 gggataggtt gttggatcga cgggccgggc tcgagcagga attcttcctg gtcgagaatt
     6601 ccggtcatcc atcggagcgg gccgacgagt tcctggaact ctgccgcaag gagtccggcg
     6661 gtcgcgtgtg cttcgccccc gagttcgtgt tgggcctggt ggaggtaaac acgcccccgg
     6721 tctacaccct ccatgacctc gagcgcgagt acgcgcagaa cctgcggatc gcgctgcgca
     6781 cctcgagatc tctgggactc aggctctacc cgctcggcac ctatcctttg cctctcgagc
     6841 cctccgtccg cgatgggctg gactacaggg tccaggtcaa tacggtcggc ccggagaggt
     6901 tcgaagatgc cggcagatgc gcgggtacgc acctacatct ggagttgcag gcagggacga
     6961 tcgacgagga agcgggcatc tccgccgccg cgtcatccgc cgccaggcag gaggtttcga
     7021 acatctacaa ccttgcgacg gccttggacc ccgccctggt ggccctcacc cgctcgtgcc
     7081 cgtttttcga agggcggaga acgggactgg ccgtccgcac cgtccattac aggggcagcg
     7141 ccatcttcgg gtgggaagga gtgtataagg atctgcccca ggttggcgcc ttgctaccct
     7201 acgccgaaga cgccgaacat ctgatccgcc agcagttcga ccgctacgag gcctggctca
     7261 gcgctatgga ccgggccggc gtacacagac gtcgcttcgc cgaggcgggc ggggacctgc
     7321 tcagaccggc ctggaatccc gtgcgcctca accgccaggg aacgctggag ttgcgcggca
     7381 tggacagcaa ctaccccgag gtgacgctgg cgacggcggc tattatcctc cgcgccgcgg
     7441 accgcgtcag gcaggataac ctcgaagtac tgccagacga cggggtgctg gctttcgagg
     7501 tcacgggaga cgtgctaagg gtgccgggct tcggtcatct cggcggggag ctgctccacg
     7561 cggcggtaac cacaggagcg ggcgacgaga ccgtggccgc ctacctggac tcgatcctgg
     7621 agttcgccgg agtagaagac gaaaggctcg ccatcctctt tcaccaccgc tgtgctacgg
     7681 gcagctaccc aaccaccgag gccggtatcg tcgaagccta cgacggacat ctctccgaag
     7741 acgaaggttt gcgcctggtg ctcgaagcct gcgacgaatt ggagacccag gtctcgcacc
     7801 tcagccgatc tcaacgccgg tacgaagagc cggcggacgt ctcataactc ttcccggtct
     7861 cctcgcgggt atactcgccg catgggcaag gaacgcgcgg ttatcggtat ggacttcggg
     7921 gtccgggttg cggcggtagt ggagcgggag ggagagctgc tcctggttcg ccaccagaaa
     7981 ccggacaggg aaccgtactg ggtgctccct ggaggcaggc tcgagccggg cgagactata
     8041 ccggaatgcg ccggccgaga actcgccgag gagacaggcc tcaccgcccg cttctccggc
     8101 gtcctctacg tgagcgagtt cctgagggag ggacgccaca cgatcgacgt catcgccagg
//
LOCUS       DATMVF010000109         5349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_424956, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000109 DATMVF010000000
VERSION     DATMVF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424956"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1078)
                     /locus_tag="VN178_05300"
     CDS             complement(314..1078)
                     /locus_tag="VN178_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWS80421.1"
                     /translation="MDTDARPVVDLREIHKSFGDHEVLKGIDLGVAPGECVVIFGRSG
                     SGKSTLLRCINLLELPTAGSVEVDGIHYDAGPNNRQRRKAVHEIRLLTGMVFQEFNLF
                     PHLSVIENVIEAPTTVKKMPRREAEELGMGFLEKVGVAEKRDQHPIQLSGGQKQRVAI
                     ARALTMQPKVMLFDEPTSALDPELIGEVLAVMKDLALEGGTTMVVVTHEMGFAREVSD
                     RMAFMHEGSLIEEGPPAELVGGAKDPRTKRFLEAVL"
     gene            complement(1068..1904)
                     /locus_tag="VN178_05305"
     CDS             complement(1068..1904)
                     /locus_tag="VN178_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWS80422.1"
                     /translation="MSTLSNIRDVLEARSARVSFRVKLALVWVLIFIVLAVFFAAFQF
                     NVAFMLEWLPYLLAGVPLTILISILSILLSVPLALLGALGRLSNNPVLYGVSGFYVSF
                     IRGTPLPVQIFFIYLGLPQLAQYAPGPLQNVFILGTVTSGVLALGINYGAYMAEIFRA
                     GIQSVGHGQIEAAQALGMTRAQTMRRIILPQAVRVVIPPTGNEFIAMIKDSSLVGLVG
                     TQELFFRALKVGRQYFQNLEMLVVAMIIYWILTSIFSYFQGRLEKRLERGYVRDGGPH
                     GH"
     gene            complement(1923..2813)
                     /locus_tag="VN178_05310"
     CDS             complement(1923..2813)
                     /locus_tag="VN178_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWS80423.1"
                     /translation="MNRKKSLGRLASMAVALAAILVVTACGGGGSAGGGNGGGGGDLL
                     QEVKDRGTLRASTDPAYPPQSFVTNNGEFKGFDIDVTEEIAKRMGVDVEWETPSWDVI
                     TAGSWSGRWDLSVGSMTVTPERSKVLYFTPAYYYTPAAAAVHEDNTDITNLDTDLDGK
                     RIGVCNACTYEFYLDRSLNIPGNYEFVVDDPQIQAYDTDSSAIQDLALGDGVRLDAAM
                     SSLTTLQEAVDSGTPIKIVGEPLYYEPLAAAIDKKAPSDPQPFVDEVSKIIKEMHKDG
                     TLTELSNKWYGYDLSKKQGA"
     gene            complement(2868..4517)
                     /locus_tag="VN178_05315"
     CDS             complement(2868..4517)
                     /locus_tag="VN178_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS80424.1"
                     /translation="MVSDGLQYDAVVIGGGHNGLVCAAYLARAGMKTVVLERRHLLGG
                     AAVTEEVWPGFRVSTASYVVSLMSARVVRELDLPRYGYHVYPLDPAYFAPFPDGSGFL
                     LWDDPARAAGEIGKINARDGQAYLEYNRELGELADLVRPLLYRTPPDPGVRSLTDLTE
                     ALYLGRYAFGNRKSITRLVDLMTMSCADFLDRFFVDERIKGALAPGGVIGMWGGPMSP
                     GSAYVLLHHRMGEIDGVTGGWGFVRGGMGGVSEAIAASARAAGAEIRTNAEVAAIDIK
                     GGRATGVTLTGGTRIEAGTVVSGAHPQTTLLSLLGAEYLPDEVVRELETFRNRSPSAK
                     VNLALSELPDFTAMPGKEPGPQHSEIMISPSLEYLERAWDDAKYGRFSEGPMIDAVIP
                     TTKDPTIAPEGRHIMTCFVQYAPYEPREGSWDGGAREALGDRVVDTIGEYAPNFRDSV
                     IERQVLTPYDLEERFGLIGGNIFQGEMSLDQLFSFRPTTELAGYKTPVEGLYLCGSGS
                     HPGGGVMGIPGLNASKVVVGDYKSAERKRRLKERLASYSRR"
     gene            4621..>5349
                     /locus_tag="VN178_05320"
     CDS             4621..>5349
                     /locus_tag="VN178_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWS80425.1"
                     /translation="MTPIARELLAPSLENFGRSSTLPAEAYTAGSVLRWEQEHFFDRG
                     WVCAGRVGGPEVAGDQRVVEWQGWLFANASGDAPTFEEHVGNLEELVRNYGCEELVIA
                     ARHDYQIAANWKILVENYHECYHCSSIHPELCEVSPPTSGGDNLEPEGAWIGGPMDLF
                     EGVETMSLDGKSHAPKLPRLTGVQDRTVYYFQLFPNLLISLHPDYVMTHRLRPLAPDR
                     TAVECEWLFSDEAVEQVGFDPSYAT"
BASE COUNT          959 a         1769 c         1650 g          971 t
ORIGIN      
        1 cggtccgctt cgtcccgaag ccagcccgat cataccctcg gctatcgacg tcttgccgct
       61 accgcggtgc ccgatcaggc acacgttcct gatagtctcc gtctctacag ccacggattc
      121 ctcctctcat gagcctaccc cttcattccc tcggggcacg tagcttatcg cagagaggcc
      181 atccgcgcag gcctgacccc acctataaag ggcacgtaac agcaagcgag aaagacttgc
      241 agattcagga cattgtaggg gcgggtttgt cgttggggct ggtttggtgt aggggcgggt
      301 ttgaaacccg cccctacaac acggcctcca ggaatcgttt tgtcctcgga tccttcgcgc
      361 ctccgaccag ctcggcggga ggaccctcct ctatgaggct gccctcgtgc atgaacgcca
      421 tacggtcgga gacctcgcgg gcgaagccca tctcgtgggt gaccaccacc atcgtcgtgc
      481 caccctccag ggcgagatcc ttcatcaccg ccaggacctc cccgatcagc tcggggtcga
      541 gcgcggaggt cggctcgtcg aagagcatga ccttcggttg catcgtgagg gcgcgcgcga
      601 tggcgacccg ttgcttctga ccaccggaga gctggatcgg gtgctggtcc cgcttttccg
      661 cgacgccaac cttctccaga aaacccatcc cgagctcttc ggcctcgcgg cgaggcatct
      721 tcttgaccgt cgtcggggcc tcgatgacgt tctcgatcac gctcaggtgc gggaacaggt
      781 tgaattcctg aaagaccatt ccggtcagca accggatctc atgcaccgcc tttcgcctct
      841 ggcggttgtt cggcccggca tcgtagtgga taccgtccac ctcgacgcta cccgccgtcg
      901 gtaactccag gaggttaatg caccgcagta gtgtgctctt ccccgagccc gagcgtccga
      961 agatgaccac gcactccccg ggcgcgacgc ccaggtcgat gcccttgagc acctcgtggt
     1021 ccccgaacga cttgtggatc tcgcgcagat cgacgaccgg gcgggcgtca gtgtccatgc
     1081 ggaccaccgt cccttacgta gcccctctca agtctcttct cgagtcgtcc ctggaagtag
     1141 gagaagatgc tggtgagtat ccaatatatg atcatcgcca ccaccagcat ctccaggttc
     1201 tggaagtact ggcgtccgac cttcaaggcg cggaagaaga gctcctgggt gccgacgagg
     1261 ccgacgagcg aggagtcctt gatcatcgcg atgaactcgt tgcccgtcgg cgggatgacg
     1321 acccttacgg cctggggaag gatgatgcgg cgcatcgtct gcgcccgcgt catccctagg
     1381 gcctgcgccg cctcgatctg cccgtgaccc accgactgga tgccggcacg gaagatctcc
     1441 gccatgtagg cgccatagtt gatccccaaa gccagcaccc cggacgtcac cgttcccagg
     1501 ataaagacgt tctgtaacgg tcctggcgcg tactgggcca gctgcgggag gccgaggtag
     1561 atgaagaaga tctgcacggg caggggcgtc ccgcggataa aggagacgta gaagccgctc
     1621 accccgtaga gcacagggtt gttggagagt cgccccaggg ccccgagcag cgcgagcggg
     1681 acggagagga ggatgctgag gatggagatc aggatggtga gcggcacgcc ggcgaggagg
     1741 tacggtagcc actccaacat gaacgccacg ttgaactgga aggccgcgaa gaataccgcg
     1801 agcacgatga agatcaggac ccacaccagg gccaacttga cgcggaagga gacgcgcgcg
     1861 gaccgcgcct ccaggacgtc ccttatattg gagagcgtcg acatggtagc ctcagacgaa
     1921 gatcaggcac cctgcttctt ggacaggtcg tagccgtacc acttattcga gagctcggtg
     1981 agcgtcccat ctttgtgcat ctccttgatg atcttgctca cctcatcgac gaacggctgc
     2041 gggtcggaag gggctttctt gtctatggcc gcagcgagcg gctcgtagta gaggggctct
     2101 ccgacgatct taatgggggt gcccgagtct actgcctcct ggagggtggt cagggaggac
     2161 atcgccgcgt ccagcctgac gccgtcgccg agcgcgaggt cctggatggc cgacgagtcg
     2221 gtgtcgtagg cctgtatctg cgggtcgtcc acgacgaact catagtttcc agggatgttg
     2281 agcgagcggt ccaggtagaa ctcgtaggtg cacgcgttgc acaccccgat cctcttgccg
     2341 tcgaggtcgg tgtccaggtt ggtgatatcg gtgttgtcct cgtgcacggc cgcggcggcc
     2401 ggcgtgtagt agtaggccgg ggtgaagtag agtaccttcg accgctcggg ggtgacggtc
     2461 atcgagccca cgctgaggtc ccaccgcccg ctccagctgc ccgcggtgat cacatcccac
     2521 gagggggttt cccactcgac gtcgacgccc atccgcttgg cgatctcctc tgtcacgtct
     2581 atgtcgaagc ccttgaattc gccgttgttg gtcacgaagg actgtggcgg gtaggccggg
     2641 tcggtcgagg cccggagcgt accgcggtcc ttgacctctt gaagtaggtc tccacccccg
     2701 ccaccgttgc caccccccgc ggagccgcct cccccacagg cggtgaccac cagtatggcc
     2761 gcaagcgcga cggccatcga cgcaagcctt cccaagctct tcttccggtt catgctcgtt
     2821 ttctccattc cctcgatacc ccttcgagac gtatgaacta tagcggttca gcggcgcgaa
     2881 tacgaggcca ggcgctcctt gagccgcctc tttcgctccg cgctcttgta gtccccgact
     2941 accaccttcg aggcgttgag gccggggatg cccatgaccc ctccgccggg gtggctgccg
     3001 gagccacaga ggtagaggcc ttccacgggc gtcttgtatc ccgcgagctc cgtggtgggg
     3061 cggaaggaga atagctgatc cagcgacatc tccccctgga agatgttgcc cccgataagt
     3121 ccgaagcgct cttcgaggtc gtagggcgtc aaaacctggc gttcgatcac cgagtccctg
     3181 aagttcgggg cgtactcgcc tatagtatcc acgaccctat cacccagagc ctctctggcg
     3241 ccgccgtccc aacttccctc tctaggctcg taaggggcgt actgcacaaa gcaggtcatt
     3301 atgtgacggc cctcgggggc gatggtcggg tctttggtcg tggggatcac cgcatctatc
     3361 atgggtccct ccgagaagcg cccgtacttg gcgtcgtccc acgccctttc cagatactcg
     3421 agggaggggc tgatcatgat ctcggagtgc tgcggtcctg gctctttgcc aggcatcgcc
     3481 gtaaagtcgg gaagctcgga gagcgcgagg ttgaccttgg ccgaaggact acggttcctg
     3541 aaggtctcga gctcccggac gacctcatct gggaggtact ccgcgccgag aagcgaaagc
     3601 agcgtggtct gggggtgggc tcccgagacg accgtaccgg cctctatgcg ggtacccccc
     3661 gtaagggtga cgccggtagc ccggcctccc ttgatgtcta tggcggcgac ctcggcgttg
     3721 gtgcggatct ctgcccctgc ggcccgtgcc gaggcggcta tcgcctcgga gacgcccccc
     3781 atcccgccgc gcacgaaacc ccagccgccg gtcacgccgt cgatctcacc catcctgtgg
     3841 tgcaagagca cgtaggccga gcctgggctc atcgggcctc cccacatgcc gatgacgccg
     3901 ccgggggcga gcgcgccctt tatccgctcg tccacgaaga agcggtcgag aaagtccgcg
     3961 caactcatcg tcatcaggtc aacgagacgc gtgatgctct tgcggttgcc gaaggcgtaa
     4021 cgcccgagat acaacgcttc ggtcaggtca gtcagggagc ggacgccagg gtccggcggc
     4081 gttctgtaga gaaggggtcg tacgagatcg gcgagctcgc cgagttcgcg gttgtactcc
     4141 aggtaagcct ggccatccct ggcgttgatc ttcccgatct ccccggccgc ccgcgccggg
     4201 tcgtcccaga gcagaaagcc cgagccgtcc gggaaggggg cgaaataggc cgggtcgagc
     4261 gggtagacgt ggtagccgta cctggggaga tccagctcgc gtacgaccct cgccgacatg
     4321 aggctgacca cgtaggaggc cgtcgagacc ctgaatcccg gccacacctc ctccgtcacc
     4381 gccgcccccc caagcaggtg gcgccgctcc agcacgaccg tcttcatgcc ggcacgcgcg
     4441 aggtacgccg cgcacacgag cccgttgtgc cccccgccga tcaccacagc gtcgtattgc
     4501 aaaccgtctg agaccattac gctccttccc cgactcccca gcatttcctg tacaattgag
     4561 gcattctagc agcaagcgcc atccacccga acctcgggac cgacacggag ggaggtccac
     4621 gtgacgccta tagcccgcga gcttctcgca ccatcgctag aaaacttcgg ccgcagcagc
     4681 acgctccctg ccgaggcgta caccgccggg tccgtgctcc ggtgggaaca agagcacttc
     4741 ttcgatcggg ggtgggtctg cgccggccgc gttggcgggc ccgaagtggc cggcgaccag
     4801 cgcgtcgtgg agtggcaggg atggctcttc gccaacgcca gtggtgacgc ccccacgttc
     4861 gaggaacacg tgggcaacct ggaggaactg gtccgaaact acggttgcga ggagctagtc
     4921 atcgccgccc gccacgacta ccagatagcg gctaactgga agatcttggt ggagaactac
     4981 cacgagtgct accactgctc ctccatacat cccgagctct gcgaggtcag cccgccgacc
     5041 agcggcggcg acaacctgga gcctgagggg gcctggatcg ggggccccat ggacctcttc
     5101 gagggcgtag agaccatgtc gctagacggc aagagccacg cgcccaaact gccccgcctc
     5161 accggggtac aggaccgcac ggtctactac ttccagctct tcccgaacct ccttataagc
     5221 ctccaccccg actacgtcat gacccaccgc ctgcggccac tagcccccga ccgcacagcc
     5281 gtggagtgcg agtggctgtt ctccgacgag gcggtcgagc aggtaggctt cgacccctcc
     5341 tacgcaacg
//
LOCUS       DATMVF010000110        17093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_425042, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000110 DATMVF010000000
VERSION     DATMVF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425042"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /gene="aspS"
                     /locus_tag="VN178_05325"
     CDS             <1..1293
                     /gene="aspS"
                     /locus_tag="VN178_05325"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWS80426.1"
                     /translation="GARDYLVPARLYPGQFYALPQSPQQFKQLLMVAGFERYFQIARA
                     LRDEDQRGDRQPEHTQLDLEMSYTTQDEILALIEGLYSGIVEQLTQKKLLAKPFPRLT
                     YAEAMDRFGTDKPDIRFGLELHDVSRLVADSEFKVFKGAVESGGSVRGIAVGGLGDLT
                     RRELDELTAVATSGGARGLAHFQVQPEGLKSPIAKFFSHEQQATLRETLGAGEGDWLF
                     FVADKNPVVFESLGRLRLHFRERLGLADPEVLGMCWVTDFPLFEWNENEGRIEPMHHM
                     FTMPREEDLPLLDSDPLQVIGQLYDLVANGTELASGSIRIHNADLQQKVFDVIGIGPE
                     EAHRRFGTILTAFRYGAPPHGGIAPGVDRLVMLLTDEPNIREVMAFPKTQAARDEMMD
                     APGPVSEEQLKELNISLRRRPGGERGSDPAARPGEGNN"
     gene            1298..1939
                     /locus_tag="VN178_05330"
     CDS             1298..1939
                     /locus_tag="VN178_05330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWS80427.1"
                     /translation="MRTDYPSPAPVALTFDDGPDPVWTPLVLEALASVGAPSTFFVIA
                     PRAARYSSLLSSMRDGGHDVALHCVEHTRHDAMTRREIEADVGAGLLALGRSVRYWRT
                     PWGFITPATEEVANRYRLRLVGWTADTEDWRGGTPQEMLARIRGGLSPGAIVLMHDGV
                     GPGSERAGCQETVDLVGPLVSVVRSHGLEPASTRPSQRKTAGLGRYARSSLYS"
     gene            complement(1912..2292)
                     /locus_tag="VN178_05335"
     CDS             complement(1912..2292)
                     /locus_tag="VN178_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80428.1"
                     /translation="MAIEDLRQSPMMAHMLDALDNGEDIGHYGRLVFAMIGRHFVSNA
                     ELVELLTKDQDTDEQEMRALVQQVEEKGYSPPRREKILEYQRQQDFPICPDPDDPDAC
                     NPYAELQFPDEVYESIQEYREERA"
     gene            complement(2441..3718)
                     /gene="hisS"
                     /locus_tag="VN178_05340"
     CDS             complement(2441..3718)
                     /gene="hisS"
                     /locus_tag="VN178_05340"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWS80429.1"
                     /translation="MTDFRGPRGTYDVFPGGRETYERAELWTFVEDRAREILARYNYS
                     EIRTPIFEEARLFARTAGEGSDIVVQKEMFTFTDKGGREMALRPEGTPGAVRAYIEHN
                     LYKLAQPIKLFYVGPMFRQERQQRGRYRQHTQIGVEALGASDPLVDVEVISLLYAIHQ
                     AVGVKDEVVYVNNLGDMETRQAYVPELRAYLQRHRDELDPDSVSRMDTNPLRTFDSKH
                     EGTQAVLDEAPKISGFLSEEASAHLGVVRQGLRALGIPHEIDDRLVRGLDYYTMTVFE
                     AKSGVLGAQDTVGAGGRYNALISDLGGPDLGGIGFGSGVERMLLAAASQEAQTSLDVF
                     FVTLAPAARIAAMGLAGALRDEGISADLDYGGRGMRAQFRQADRTGASYAAILGEDEL
                     ARGVCTVRDMASGEERVVPVAEGAKDLIRAVSG"
     gene            complement(3715..4290)
                     /locus_tag="VN178_05345"
     CDS             complement(3715..4290)
                     /locus_tag="VN178_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80430.1"
                     /translation="MSDIEQVTLSMDGFEVNSYVVHAPDGDVIVDAGAEPEKILGATR
                     ARVAAILVTHGHADHIDALERVRRETGAPVYMHPADAEGKVRDYVPLEDGQALDVAGL
                     GVRVLHTPGHSPGSVTFVVGHDQIVGDLILPGSVGRTDIPGASWEEIEDSIRRVIPLW
                     EEGTRLYAGHGPVLAAVEELRTNPYLPPGLS"
     gene            complement(4302..6527)
                     /locus_tag="VN178_05350"
     CDS             complement(4302..6527)
                     /locus_tag="VN178_05350"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWS80431.1"
                     /translation="MEPVRTEERPKAIPAPGVTISSLIEKVSSYAPDGTEELIADAYN
                     AAHAAHRGQTRKSGEPFVYHPLATADVLAELRLDPTTISAALLHDVLEDTGVTKEELS
                     ERFGDEVAEIVDGVTKLKRLPSGNLEEAQAESLRKMIVAMSRDVRVIIIKLADRLHNM
                     RTLAYLKRETQLRKATETLEIYAPLAHRLGIYSIKWELEDLSFATLHPRRYEEIKSLV
                     AARRGDREAFINGTAAELLRHLRETGIEAEVHGRVKHFYSIYNKMVRRNKEFNEIYDL
                     AGLRVVVDDVRDCYGTLGVIHSIWKPIPGRFKDYIAMPKFNMYQSLHTTVMSNEGKLL
                     EIQIRTAEMDTTAEYGIAAHWMYKHGLTDRQVDRLTWLKSMMEWQQETTDASDFMESL
                     KGELGAEEVFVFTPKGDVVSLPSGATPIDFAYHVHTEVGHRTIGARVNDRIVPLDSEL
                     VSGDRVEVITGKNSGPSRDWLAVVQSGRARNKIRQFFNKADREDNLSLGREKILTLLK
                     KRRIGKIPSGILEDVARATNQSSPDDMFAAVGAGVLSAENVAGRLVEQLRPKEEEERP
                     RERSPALAPLPLPESGTDEQTGVRVVGSSGILTRLARCCTPMPGDAIVGYVSLGRGVV
                     VHSAGCVNAGALQARDPERFVEVEWAQGSDKLFTVELLVEALDRMHLLKDITTTISDA
                     GVNIVSARVDTIEDRTALSRFAFKAASVQHVEEVIRKIRSIPDVYDVYRVSRDGTPLE
                     R"
     gene            complement(6705..8375)
                     /locus_tag="VN178_05355"
     CDS             complement(6705..8375)
                     /locus_tag="VN178_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWS80432.1"
                     /translation="MANGRISRAARSGNLRRFSQISRVLVRHGFGFVFDVRRDRREKK
                     GLQQVLAPNFGVRLRLTLDDLGPTFVKFGQVLSTRSDILPEGVLSELQKLQDTATPMP
                     AGVAQEIIEDELGAPVDELFASFDPVPLGSASIGQVHRAVLRSGESVAIKVQRPEAPR
                     RVAGDLELMREFAALLDRRFGRRLFVDVRGLVAEFEVVIRRELDYTAEAENARRFAAN
                     FSGTQVVIPGVYLGLSTPKVLTEEFIEGTRFRDVRPLMLSPSERRRVASMGAEAIFKM
                     AFEDGFFHGDPHPSNLLLTPGGDLALLDFGMVGYMSQGDIEALSRLFIAVVQRDAAAA
                     LRALERLGVSYATEVRGDLIRDLREFLNKYSGLSVGEVTLGQALSELVALARRYKLRM
                     PPVFPLLTKALVTAEGLARSIDPTINVYEVARPYAQRLLSGRYRPQVVAEAAEERALE
                     YARYVEDYPEQIRQLLTEIADGELEVQLKHHGLNELFGSVDVLANRLVFAVVTAALFV
                     GSSMLGAFYVAGPQVPFVGAPLVSFLGFTLSIIQTIILLTIIFRSGRL"
     gene            complement(8389..8790)
                     /locus_tag="VN178_05360"
     CDS             complement(8389..8790)
                     /locus_tag="VN178_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80433.1"
                     /translation="MRGTLGETLDRLILLQIGAAAATRDRVQGIVDSLVEQGRIEREE
                     GRTVVNDVMNRARERSTGARSLVDASLQQGLRRGGVPTREDYEDLLFRIEQLEHRVRM
                     LEDRPAAPPPPAGEPEAPPERGDDPEPPPGQ"
     gene            complement(8870..9760)
                     /gene="secF"
                     /locus_tag="VN178_05365"
     CDS             complement(8870..9760)
                     /gene="secF"
                     /locus_tag="VN178_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWS80434.1"
                     /translation="MFEKVDFVGGWKLYFSISAVLLLAGTIAIALGGLNFGIDFQGGA
                     QFTVANAERQLSDDEVRAALPDDLAGEAVVTTLGQNGYEVRTPVISSQQEQSRVRDSL
                     SQAFGGEVSVTTVSPAFGEQLRNQALQAVVAALIIIVAFISFRFEFAFALAAMAALVH
                     DVLMTVGFYAIVGREVSLVTVVAVLTVLGYSIYDTIIIFDRIRENAPTVGYNRRRFDE
                     MINRSIRQVVRRSIYTSIFTLIPITALLIFGEATLSDFAFALLVGIVAGTYSSIFIAS
                     PVLSLYKAWRAGKLQAARST"
     gene            complement(9762..11255)
                     /gene="secD"
                     /locus_tag="VN178_05370"
     CDS             complement(9762..11255)
                     /gene="secD"
                     /locus_tag="VN178_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWS80435.1"
                     /translation="MGSRRNNLIVLAVVVVLLGVAAYFIFIDQPVTESTQLGLDLQGG
                     VSAQLEGSQTGGGKVTRQEMEQSAELIRQRIDRLGVTEPDVRVQAENQIVVQIPGVKN
                     PEEVIQIIGSTAQLGFYEVLASEPTLTEPPQVVAADEVSAIRNDIRETLEGDDAYEKG
                     KTKILFSETPSLQGDGIEVIGYIVNQEPDMTGDALKQNGASVEFDQTTGKRIVSLELT
                     AEGGRQMGELTQSMVNDSLASGEPARLAIALDEDIQSAPTVQEPLGQNISISNDGLPG
                     GLPEDEARQLETVLNTGALPVNMEVISQTTVGPTLGLSSLKSGLLASLVGFGLVLLLL
                     LVIYRALGIVADVALLIYAFLLWGLIVIIPITVTLPGIAGIVLSIGVAADANVVIFER
                     IKEEVRRGKTPRSAIQAGYDKGFRAILDGNVTTLITAFILFALSSGSVRGFAVLLSIG
                     VVLSMFTAIVITRALLGLLSDRGIHLSPALMGVSKASISAAQQARVK"
     gene            complement(11268..12389)
                     /gene="tgt"
                     /locus_tag="VN178_05375"
     CDS             complement(11268..12389)
                     /gene="tgt"
                     /locus_tag="VN178_05375"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWS80436.1"
                     /translation="MEVHARDGEARAGVLRTPRGRVETPAFMPVGTKGTVKGLTPGDL
                     REVGAGIVLANTYHLYLRPGSDLIKKAGGLHAFMGWEGPILTDSGGYQVFSLANTRRI
                     SEEGVEFASVYDGSPHVFTPELTTKVQEDLGSDVAMVLDECPPANASREYHEQSLRRT
                     ARWAYRCKEAHERRDQALFGIVQGGLDPGLREESLGLTVEIGFDGYAVGGLSVGESRE
                     EMLKTLALTAPRLPAGKPRYFMGIGDPVGILKVIALGVDMFDCVLPTRLARHGAALTQ
                     DGRINLKNARYRTDFGPLDPGCSCEACAGYSRAYLAHLVRENELLGHRLITLHNVRFS
                     VDLCRLARREIGAGRFEEWSRAWISRYESSDRAGADRLE"
     gene            complement(12404..13414)
                     /gene="queA"
                     /locus_tag="VN178_05380"
     CDS             complement(12404..13414)
                     /gene="queA"
                     /locus_tag="VN178_05380"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWS80437.1"
                     /translation="MLVSALDYELPEDLIAQSPAEPRDASRLMVVDVGRNTIAHHTFS
                     DLPDFLRPGDALILNETKVIPARVEARKSTGGAIELLFLKDLGPDLDGAWEVLARPSK
                     RLRPGLTLSAGGVRLRLVKSLGNGHWVVSASNVRGLLERGGRMPLPPYIRPTPETESR
                     YQTIYARNEGSAAAPTAGFHFTKRVLQGAEQAGARIGRITLHVGVGTFMPVRTERLEE
                     HRMHAEHYSVPPEAAGVVKNASRVVAAGTTVARTLETWAATGEREGESELFVYPGYRW
                     LAVDALLTNFHLPRSTLLAMVMSFGGEDLVREAYRTAVRERYRFYSFGDAMLLLDGGR
                     QR"
     gene            complement(13416..13634)
                     /locus_tag="VN178_05385"
     CDS             complement(13416..13634)
                     /locus_tag="VN178_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWS80438.1"
                     /translation="MGLENVGKLLLGGAVVLLILGGLFLLLGRLGIDRLPGDLVFRRG
                     NATFYFPIGLMILLSIVGTLVLNILFRR"
     gene            13773..15395
                     /locus_tag="VN178_05390"
     CDS             13773..15395
                     /locus_tag="VN178_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWS80439.1"
                     /translation="MGRTKYAIAFALALVMALGAVGVASAAVPVDSSQLRDAVGVGGI
                     LEHERAFQAIADSNGGTRASGTPGYDESADYVAEKLESAGYSVTRQVFDYDLFIENSE
                     PVLDPTDPDLPAYTPGDMPDDDFSTMSYSGSGDVTAPLQAVGGIIIPSPGGSASGCTA
                     EDFAGFEAGNIALIQRGTCNFREKVENAQAAGAVGVVIFNEGNVVPDDDRVGNLFGTL
                     DPPQMKVPVVGTSFAVGEELYNLLQQGPVTLRLVVDATVEQRTTENVIADTTTGRADR
                     TVVVGAHLDSVPEGPGINDNGSGSATILEVAEAMSELDIQPRNRVRFAFWGAEESGLI
                     GSEYYVSQLPKRELKDIAVNLNFDMVGSPNYVRFVYDGDASDTNSLGSTGSGVVEDVF
                     TDYFASQGLASEPTAFDGRSDYDAFISVGIPAGGLFTGAEDIKTAEEAETYGGAADVA
                     YDPCYHQACDSFTPLADGADPAAYAAIEAGYPPGTLVGNLNTQALDEMSDATAHGTLT
                     FAQTTSAVTGTNKGSGAPMSSAAKYHGPLLQR"
     gene            complement(15460..15972)
                     /locus_tag="VN178_05395"
     CDS             complement(15460..15972)
                     /locus_tag="VN178_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS80440.1"
                     /translation="METSERTRRVVNPGTSIGHVHLKVANIERALGFYRDILGFEVTQ
                     WYGDDAVFLSAGGYHHHIGLNTWMSRNAGPAPTHSAGLFHLAILYPERRDLAQALKWL
                     LESNYPIDGASDHGVSEALYLRDPDGNGVELYRDRPQDEWPRTADGELAMVTRPLDVD
                     GVLRELDEAR"
     gene            16300..>17093
                     /locus_tag="VN178_05400"
     CDS             16300..>17093
                     /locus_tag="VN178_05400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWS80441.1"
                     /translation="MEITDTRHRSRTILEGRDRAPARSFLKGMGFSDEDLSKPIVGVA
                     HSWIETMPCNYNHRHLAEKVKEGIREAGGTPMELNTISISDGITMGTQGMKSSLVSRE
                     VIADSIELVGRGHMFDAMVTIVGCDKTIPAAAMAHARLGIPGAIVYSGSIAPGRFKGH
                     DVTIQDVFEGVGANAAGRMSDEDLLDLEEHACPGAGACGGQFTANTMAMILEFLGLSP
                     MGSAGPPAVDGRKDEVCARAGRLVMELLEHGLTPERILTREAFENG"
BASE COUNT         3067 a         5592 c         5371 g         3063 t
ORIGIN      
        1 ggcgcgaggg actacctggt ccctgcccgc ctctatccgg ggcagttcta cgcgcttccg
       61 cagtctccgc agcagttcaa gcagctcctg atggtggccg gcttcgaacg ctacttccag
      121 atagcccgcg cgcttcgcga cgaggaccag cgcggggacc gccagcccga acacacgcag
      181 ctcgacctgg agatgagcta cacgacgcag gacgagatcc tggccctcat cgaaggcctc
      241 tactccggga tcgtcgagca gcttacgcag aagaagctac tcgccaaacc gttcccccgc
      301 ctaacctacg ccgaggcgat ggaccgcttc gggaccgaca aaccggacat ccggttcggc
      361 ctggagctcc acgacgtctc tcggctcgtc gccgactcgg agttcaaggt tttcaagggg
      421 gccgtcgaat cgggcggctc ggtgcgcggc atcgccgtcg gggggctggg agacctgacc
      481 cgcagggagc tcgacgagct caccgcggtc gcaacctcgg gaggggcaag gggcctggcc
      541 cactttcagg tgcaaccgga gggcctgaaa agccccatcg ccaagttctt ctcccacgag
      601 caacaggcca ctctgcgcga gacactcgga gccggagaag gcgactggtt gttcttcgtc
      661 gcggacaaga accccgtggt cttcgagagc ctgggccgcc tgcgactcca cttcagggag
      721 cgcctcgggc tcgccgaccc cgaggttctc ggtatgtgct gggtgacgga cttccctctc
      781 ttcgagtgga acgagaacga agggcgcatc gagccgatgc accacatgtt caccatgccc
      841 cgcgaagagg acctgccgtt gctcgactcc gaccccctgc aggtcatcgg ccagctttac
      901 gacctcgtgg ccaacggcac cgagctcgca tcggggagca taaggatcca caacgccgac
      961 ctgcagcaga aggtcttcga cgtcatcggt ataggacccg aggaggcgca caggcggttc
     1021 gggaccatcc tcaccgcctt ccgctacggc gcgccgcccc acggtgggat cgcaccgggg
     1081 gtggaccggc tggtgatgct cctcacggac gagccgaaca ttcgagaggt gatggcgttc
     1141 cccaagaccc aggccgcccg cgacgagatg atggacgccc ccggtcccgt ttcggaggag
     1201 caactcaagg agctgaacat ctcgctccgc cgccgccccg ggggcgaacg aggctccgac
     1261 cccgcggcca ggcccggtga gggcaacaac tagagcgatg cgcaccgact acccgtcccc
     1321 ggcgcccgta gccctcacct tcgacgatgg gccggatccc gtctggactc ccctcgtact
     1381 ggaggccctg gccagtgtcg gggcaccctc cacattcttc gtcatcgcgc cacgggccgc
     1441 acgttactct tcactcctct ccagcatgcg cgatggaggc cacgacgttg cccttcactg
     1501 cgtagagcac acccgccacg acgcgatgac acggagagag atcgaagcag acgtaggagc
     1561 aggactcctg gcgctcggac gctccgtgcg atattggagg acgccatggg ggttcatcac
     1621 accggccacc gaagaggtgg caaacagata ccggctccgt ctcgtcggtt ggaccgctga
     1681 cacggaggac tggcggggag gtacgccgca ggagatgcta gcccgcataa ggggtgggct
     1741 ctcgcccggg gccatcgtac tgatgcacga cggagtcggc cctggaagtg agcgtgcggg
     1801 ttgccaagag accgtggatc tcgtgggacc gctcgtctct gtggtgagat cccacggcct
     1861 ggagcccgcg agcacgcggc cttcgcaaag aaagaccgcg gggctagggc gctacgcccg
     1921 ctcctccctg tactcttgaa tgctctcgta gacttcgtcg gggaattgca gctccgcgta
     1981 cgggttgcag gcgtcagggt cgtcgggatc ggggcagatc gggaagtcct gctgcctctg
     2041 atactccagg atcttctcgc gcctgggcgg gctgtagccc ttctcttcca cctgctgcac
     2101 gagagccctc atttcctgct cgtcggtatc ctggtccttg gtcagaagct ctacaagctc
     2161 ggcgttggag acgaagtgac ggccgatcat cgcgaagacc aggcgcccgt agtggccaat
     2221 gtcctcaccg ttgtcgagcg cgtcgagcat gtgggccatc atcgggctct ggcgcaaatc
     2281 ttctatcgcc attaactgtg ctcctctcac tagcgtacac cccgaacaat acggggacct
     2341 gctgtactga atgtgaaacc ggctactccc acagcagtca gaccgaccat cgaagacgta
     2401 cgccgccgtg cggcgcttcg tctggtcggg ccgccaacgc tcagccggag acggcccgga
     2461 tcaggtcctt cgccccttcg gcgacaggca cgacgcgctc ctctcccgag gccatgtccc
     2521 tcaccgtgca caccccacgt gcgagttcgt cctcaccgag tatggcggcg tacgaggcgc
     2581 ccgtacggtc ggcctgcctg aactgcgcgc gcatgccgcg ccctccgtaa tccaggtcgg
     2641 cggagattcc ctcgtccctg agggcgccgg ccaaccccat ggcggcgata cgggccgcgg
     2701 gggccagggt cacgaagaac acatcaaggc tggtctgcgc ctcctgcgaa gcggcggcga
     2761 ggagcatgcg ctcgacgccc gacccgaagc cgatcccccc aaggtcgggc cctccgagat
     2821 cggagatcag ggcgttgtac cggccgccgg ccccgacggt gtcctgcgcc cctaggacgc
     2881 cgctcttggc ctcgaagacc gtcatcgtgt aatagtcgag ccctcgcacg agcctgtcgt
     2941 cgatctcgtg gggtatgccc agggcccgga gtccctggcg cacgacaccg aggtgcgcag
     3001 aggcctcctc gctcaggaaa ccggagatct tcggcgcctc gtcgagaacg gcctgggtgc
     3061 cctcgtgctt ggagtcgaag gtgcgcagcg ggttcgtgtc catgcgggac acggagtcgg
     3121 ggtcgagctc gtcgcggtgc ctctggaggt aggcgcgaag ctcggggacg taagcctgcc
     3181 tggtctccat atccccgagg ttgttgacgt agaccacctc atctttcacg ccgacggcct
     3241 ggtggatggc gtagagaagt gagatcacct cgacgtcgac gagcgggtcc gaggcgccga
     3301 gggcctcaac cccgatctgg gtgtgctgcc tgtagcggcc cctctgctgg cgttcctggc
     3361 ggaacatcgg accgacgtag aacagcttaa tcggctgcgc aagcttgtac aggttgtgct
     3421 cgatgtatgc ccgcacggca cccggagtcc cctcgggtct gagcgccatc tccctgccgc
     3481 ctttatccgt gaaggtgaac atctccttct ggacgacgat gtccgagccc tccccagccg
     3541 tcctggcgaa cagtctcgcc tcctcgaaga tgggcgtgcg gatctcggag tagttgtagc
     3601 gggcgagaat ctcgcgggcc ctgtcctcga cgaaggtcca caactctgcc ctctcgtaag
     3661 tctcccgtcc gccagggaag acgtcgtacg tgcccctcgg tcctctgaag tccgtcacga
     3721 gagtccggga ggcagataag gattcgttct caactcctcg accgccgcaa ggacagggcc
     3781 gtggcccgcg tagaggcgcg ttccctcttc ccagagcggg atcaccctgc ggatggagtc
     3841 ctcgatctcc tcccacgaag cgccgggtat gtctgtccgg cccacgctcc ccggcaagat
     3901 cagatcaccc acgatctggt cgtgtcctac gacgaacgtc acggagcccg gggaatgtcc
     3961 cggcgtgtgg agaaccctga cgccgagacc ggcgacatcg agcgcctgcc cgtcttcgag
     4021 tggcacgtag tctctgacct tcccctcggc gtccgcgggg tgcatgtaga cgggggcgcc
     4081 agtctctctg cgtacccgct ccagggcatc gatgtggtcg gcgtggccgt gggtcaccag
     4141 gatcgcagcc acacgggcgc gggtggcgcc gaggatcttc tccggctcgg caccggcgtc
     4201 gacgattacg tcgccatcgg gagcgtggac gacgtaagag ttgacctcga agccgtccat
     4261 agagagtgtg acctgttcga tgtcgctcac aggcgagcct cctagcgctc caggggtgtg
     4321 ccgtcgcggg agaccctgta gacgtcgtag acgtcgggga tgctccggat cttgcgtatt
     4381 acctcctcga cgtgctgcac gctcgcggct ttgaatgcga acctgctcag tgccgtcctg
     4441 tcctcgatgg tatcgaccct cgcagagacg atgttgacgc cggcgtccga gatcgtggtc
     4501 gttatgtcct tcaacagatg catccggtcg agggcctcga ccagcagctc gacggtgaag
     4561 agcttgtcag aaccctgggc ccattcgacc tcgacaaagc gctcggggtc cctggcctgg
     4621 agcgctccgg cgttcacgca gccggcggaa tggactacga cgccccgtcc cagggagacg
     4681 tatccgacga tcgcgtcgcc gggcatcggg gtgcaacagc gtgccagccg cgtcaggatc
     4741 ccgctcgacc cgacgacgcg cacgcctgtc tgctcgtcgg ttccactctc ggggaggggg
     4801 agcggggcga gcgcggggct ccgctctctc ggcctttcct cttcctcctt tggccgtaac
     4861 tgctcgacca ggcgtcccgc cacgttctcg gccgagagca caccggcccc gacggcggcg
     4921 aacatatcat ccggcgagga ttggttggtc gcgcgggcca cgtcctccag aatgcccgag
     4981 gggatctttc cgatccgccg ctttttgagg agggtcagga tcttctcgcg tcccaggctc
     5041 aggttgtctt cccggtctgc cttattgaag aactgcctga tcttgttcct cgcgcgtccg
     5101 ctctggacca ccgccagcca gtcgcggctc ggccccgagt tcttgcccgt aatgacctcg
     5161 accctgtcgc cggagacgag ctcggagtcg agggggacga tacgatcgtt caccctggcc
     5221 cctatcgtgc ggtggccgac ttcggtgtgg acgtggtagg cgaagtcgat cggtgtggcc
     5281 ccgctcggaa ggctgaccac gtcgcccttc ggcgtaaaga cgaagacttc ctcggcgccg
     5341 agttcaccct tcaacgactc catgaagtcg ctggcatccg tcgtctcttg ctgccactcc
     5401 atcatgctct tgagccaggt cagccggtcc acctgacggt cggtaagacc atgcttgtac
     5461 atccagtggg cggcgatgcc gtactccgcc gtcgtatcca tctcggcggt ccggatctgt
     5521 atctccagta gcttcccctc gttggacatc acggtcgtgt gaagcgactg gtacatgttg
     5581 aacttgggca tcgctatgta gtctttgaaa cgcccgggga taggcttcca gatcgagtgt
     5641 atgacgccaa gggtgccgta gcagtctcgc acgtcgtcga cgacgaccct gaggccggcc
     5701 aggtcgtaga tctcgttgaa ctccttgttg cgacgcacca tcttgttgta gatggagtag
     5761 aagtgcttga ccctgccgtg gacctcggcc tctatccccg tctcccgcaa gtgccgcaag
     5821 agttcggccg ccgtcccgtt gataaacgcc tccctgtccc ctcgcctggc ggcgacgagg
     5881 ctctttatct cctcgtagcg tcgcgggtgt agggtggcga acgagaggtc ctcgagctcc
     5941 cacttgatgg agtagatccc aagcctgtgg gcgagggggg cgtagatctc cagtgtctcc
     6001 gtcgccttcc tcagttgggt ctcacgcttg aggtaggcga gggtcctcat gttgtgcagc
     6061 ctgtcggcga gcttgatgat gatcacccgc acgtcgcggc tcatggccac gatcatcttc
     6121 ctcaaagact cggcctgcgc ctcttcgagg ttgccggaag gaagccgctt gagcttggtt
     6181 accccgtcga cgatctcggc cacctcatca ccgaagcgtt ccgacaactc ctctttggtt
     6241 acgccggtat cctcgagcac atcgtgcaag agcgcggcgg agatggtcgt ggggtcgagg
     6301 cgcaactcgg cgaggacgtc ggccgtcgcg agggggtggt agacgaaagg ctcgccgctc
     6361 ttgcgtgtct ggcccctgtg cgccgcgtgc gcagcgttgt aggcgtcggc gatcaactcc
     6421 tcagtcccat cgggcgcgta ggaagatacc ttctcgataa gagaagatat ggttacgcca
     6481 ggcgccggta tggccttcgg gcgttcctct gtacgaacgg gttccacaca gaaattatat
     6541 cagccgtcag ctatcagccg tcagccatca gtagttgacc aaaaccgcat agattggcgt
     6601 cgcagaggaa tgtgtctggt gcgcagtcga gcggtatagg cttcagccta cactcgctgc
     6661 gcttctagac gtagctgaga gctgagtgct gaaagctgac tgctctatag ccttccagac
     6721 ctgaaaataa tcgtgaggag gatgatggtc tgaatgatgg agagcgtaaa cccgaggaag
     6781 ctgacgaggg gcgcgccgac gaagggcacc tgcgggcctg cgacgtagaa agcgcccagc
     6841 atcgaggaac cgacgaacag ggcggccgta acgacggcga agaccagccg gtttgcgaga
     6901 acgtccacgc tgccgaacag ttcgttgagg ccgtggtgct tgagttgcac ttcgagctca
     6961 ccgtcggcga tctcggtgag tagctgccgg atctgctcgg gatagtcctc gacgtagcgg
     7021 gcgtactcca gagcacgctc ctcggcggcc tctgccacga cctgtggcct gtagcgtccg
     7081 gagaggagcc tctgggcgta gggcctggcg acctcgtaga cgttgatggt cgggtctatg
     7141 gagcgggcga gtccttctgc ggtcactagc gccttggtga gcagagggaa gacgggcggc
     7201 atcctgagct tgtagcggcg cgcgagcgcg acgagttccg agagcgcctg gcccagggtg
     7261 acctcgccca ctgagagccc ggagtatttg ttgaggaact cccgcaggtc ccgaatgagg
     7321 tcgccgcgta cctcggtcgc gtagctcacg ccgagccgct cgagggcccg cagggcggcg
     7381 gcggcatcac gctgtaccac ggctataaag agcctcgaga gcgcttcgat gtcgccctgg
     7441 ctcatgtagc cgaccatgcc gaaatccagc agggcgaggt ccccgcccgg cgtcaggagc
     7501 agattgctcg ggtgggggtc cccgtggaag aatccgtcct cgaaggccat cttgaagatc
     7561 gcctctgccc ccatagaggc gactcgccgc cgctcggagg gcgagagcat gagcggccgg
     7621 acgtccctga acctggtccc ctcgatgaac tcctccgtga gcaccttcgg ggtggagagc
     7681 ccgaggtaga ccccggggat aaccacctgc gtccccgaga agttggcggc gaagcggcgc
     7741 gcattctctg cctctgccgt gtaatccagc tcgcgcctga taacgacctc gaactcggcc
     7801 accaggccgc gcacgtccac aaagagcctg cgcccgaacc tcctgtcgag caaagcggcg
     7861 aactcacgca tgagctcgag gtccccggcc accctacggg gagcctcggg tcgctgcacc
     7921 ttgatggcca cgctctcccc gctacgcagc acggccctgt ggacctgtcc gatgctcgcc
     7981 gagccgagcg gcacggggtc gaaagaggcg aacaactcat cgacgggggc ccctagctca
     8041 tcctcgataa tctcctgggc gacccctgcc ggcatgggcg ttgccgtgtc ctggagcttc
     8101 tggagctcgg agagcacgcc ttcgggcagg atgtccgagc gtgtggagag gacctgcccg
     8161 aacttgacga aggtcggtcc cagatcgtcg agggtgagcc gcaacctgac gccgaagttc
     8221 ggcgcgagga cctgctgcag gcccttcttc tccctcctgt ccctcctgac gtcgaagacg
     8281 aagccgaagc cgtggcgcac cagaacgcgg ctgatctggg agaagcgcct gaggttcccc
     8341 gaccgggcgg cccgggagat cctaccattc gccacgcgcg cccttttgct actgtcccgg
     8401 cggcggctcg gggtcgtctc cgcgctcggg cggagcctcg ggttcgcccg ccggcggagg
     8461 aggcgccgcg gggcggtcct cgagcatcct caccctgtgc tccagctgct cgatgcgaaa
     8521 gagcaggtcc tcgtagtcct cccttgtggg aacaccgccc ctccttagac cctgctgcaa
     8581 agaggcgtcg accaaagaac gcgcccccgt cgaacgctcg cgggcgcggt tcatcacgtc
     8641 gttgaccacc gtgcggccct cttcacgctc gatgcggccc tgctctacga gagagtccac
     8701 gatcccctgc accctgtccc gcgtcgcggc ggcggcgcct atctgcagca ggatcaaacg
     8761 gtccagcgtc tcgccaagcg ttccacgcat acctacctcc atgtatcggg tccacctcct
     8821 acatcataac caaggcgcaa gccctctggc cacgtagcaa caagcgccgc tacgtggagc
     8881 gtgcggcctg gagctttccg gcgcgccagg ctttgtagag cgagaggacc ggtgatgcga
     8941 tgaagatgga gctgtaggtg cccgcgacga tgccgacaag aagggcgaag gcgaagtcgc
     9001 tcagggtcgc ttcaccgaag atcaggagcg cagttatggg gatcagggtg aagatggagg
     9061 tgtagatcga acgcctcact acctggcgga tggagcggtt tatcatctcg tcgaagcggc
     9121 gcctgttgta gcccacagta ggggcgttct ccctgatcct atcgaagatg attatggtgt
     9181 cgtagatcga gtacccgagc accgtgagga cggcgacgac ggtgaccagg ctgacctccc
     9241 tgccgacgat ggcgtagaaa cctaccgtca tcagtacgtc gtgaacgagc gcggccatcg
     9301 cggccagggc gaaggcgaac tcgaaccgaa agctgataaa agccacgatt atgatcagcg
     9361 cggccaccac tgcctgcagc gcctggtttc tgagctgctc gccgaatgct gggctcacgg
     9421 tcgtcacgct gacctccccc ccgaaggcct ggctcaggga gtcgcgtacc cggctctgct
     9481 cctgctggga gcttatgacc ggagtccgca cctcgtagcc gttctggccc agggtcgtta
     9541 cgacggcttc ccccgcaagg tcgtccggca gcgcggcgcg cacctcgtcg tcgctgagct
     9601 ggcgctcggc gttggcgacg gtgaactgcg cgccgccctg gaagtctatc ccgaagttga
     9661 ggccgcccag cgctatggcg atggtccccg caagcagcaa gacggccgag atcgagaaat
     9721 acagcttcca gccgccgacg aagtctacct tctcgaacac gctacttcac cctggcctgt
     9781 tgcgccgcgg agatactggc cttggagacc cccatcaggg cgggtgagag gtggatgccg
     9841 cggtcagaga gcaaccccag gagtgcgcgg gtgatcacga tggccgtgaa catggagagc
     9901 acgaccccga tggagagcag cacggcgaag cccctgaccg atccgctgga gagcgcgaac
     9961 aggataaacg ccgtaatgag ggtcgttacg ttgccgtcca ggatagccct gaagcccttg
    10021 tcgtagccgg cctgtatcgc gctccgcggc gtcttgcccc gtctaacctc ctccttgatg
    10081 cgctcgaaga tgacgacgtt cgcatcggca gcgaccccga tggagagcac gatgccggcg
    10141 atccccggca gtgtcacggt gatggggatg atcacgatca aaccccagag cagaaacgcg
    10201 tagatgagca gggcgacatc tgcgactatg ccgagcgccc tgtagatcac gagcagcaag
    10261 agaagcacga gcccgaaccc gacgagcgag gccagaagcc cgctcttcag ggaactaagg
    10321 cccagcgtcg gccctaccgt ggtctgcgag atcacctcca tgttgacggg aagcgcgccc
    10381 gtgttgagca cggtctcaag ctgcctggcc tcgtcctcgg gaagtccacc aggtagcccg
    10441 tcattggaga tagagatgtt ctggcccagt ggttcctgca cggtaggggc gctctggatg
    10501 tcctcgtcga gcgcgatggc gagccgcgca ggctccccgc tggcgagcga atcgttcacc
    10561 atgctctggg tgagttcccc catctgcctg ccgccctccg ccgtgagctc caggctaacg
    10621 atgcgcttcc ccgtggtctg gtcgaactcc acgctggccc cgttctgctt gagcgcatcg
    10681 cccgtcatgt caggctcttg gttgactatg tagccgatga cttcgatgcc gtcgccctgc
    10741 agggaaggcg tctcgctgaa cagtatcttc gtcttaccct tctcgtatgc atcgtccccc
    10801 tcgagggtct ccctgatgtc gtttcgtatt gcggacacct catcagcggc gacgacctgg
    10861 ggaggctccg taagcgtagg ttcggaggcg aggacctcat agaagccgag ctgtgccgta
    10921 ctaccgatga tctggatcac ctcttcgggg ttcttcacgc cggggatctg gaccacgatc
    10981 tggttctccg cctgcaccct gacgtcgggc tcggtcacgc cgaggcggtc tatgcgctgc
    11041 cggatgagtt ctgccgactg ctccatctcc tggcgggtca ccttgccccc gcctgtctgg
    11101 gatccctcta gctgcgcgct gacaccaccc tggaggtcga ggccgagctg ggtcgactcg
    11161 gtcacgggct gatctataaa gatgaaatag gcggccaccc cgagcaggac gaccacaacg
    11221 gccagcacga tcaggttgtt tcgacgacta cccataagat cggaagatca ttctagccta
    11281 tctgcccccg cacggtcaga actttcgtat cgggagatcc aggcccgcga ccactcctca
    11341 aagcggcccg ctcctatctc acgccgcgcg agccggcaaa ggtctacgct aaaacggacg
    11401 ttgtgcagtg taatgagcct gtggccgagt agctcgttct cccttacaag atgcgccagg
    11461 taagctctgc tgtagccggc acacgcttcg caggaacaac caggatcgag cggcccgaag
    11521 tctgtgcggt acctcgcgtt cttgaggttg atgcggccat cctgggtcag ggccgcgccg
    11581 tggcgggcca ggcgcgtcgg gagcacgcag tcgaacatat ctaccccgag cgcaataacc
    11641 ttgaggatgc ctacaggatc ccctatcccc ataaagtacc tcggcttccc ggcgggcagc
    11701 cttggcgctg taagcgccag agtctttagc atctcctctc gcgactcgcc gaccgagagc
    11761 cctcccacgg cgtagccgtc gaacccgatc tcgaccgtca gcccgaggct ctcctccctc
    11821 aggccgggat ccaacccacc ctgcacgatc ccgaagaggg cctggtctct cctttcgtgc
    11881 gcctccttgc accgatacgc ccatcgcgcg gtccgccgca acgactgctc atgatactca
    11941 cggctcgcat ttgccggcgg acactcgtcg aggaccatcg ccacgtccga gcctaggtcc
    12001 tcctgaacct tcgtcgtcaa ctcgggggtg aacacgtgcg gcgagccgtc gtagaccgag
    12061 gcgaactcga caccctcctc tgagatacgc cgcgtgttgg ccagcgagaa gacctggtag
    12121 ccgcccgagt cggtgaggat ggggccctcc cagcccatga aagcgtgaag cccaccggcc
    12181 tttttgatga ggtccgatcc cggcctcagg taaaggtgat acgtgttcgc gagcacgatg
    12241 ccggccccga cctcccgcaa atctccagga gtgagaccct tgacggtccc cttcgtccct
    12301 acaggcatga aggccggcgt ctccacccgc ccacgcggcg tacgaaggac gcccgcgcgc
    12361 gcctcaccgt cacgtgcgtg tacctctatg gagactgggg cgcctaccgc tgccgtccgc
    12421 catcgagaag gagcatcgcg tccccgaaag agtagaagcg gtagcgctcc cttactgcgg
    12481 tcctgtacgc ctccctgacc aggtcctcgc ccccgaagct catcaccatc gccaggagcg
    12541 tggagcgcgg caggtggaag ttcgtcagca gcgcgtccac ggctagccaa cggtagccag
    12601 gatacacgaa gagctcggac tccccttccc tctctccggt cgcagcccaa gtctcgagcg
    12661 tgcgggcgac ggtagtgccc gctgcgacga cgcggctggc gttctttacg acccccgccg
    12721 cttccggcgg cacgctgtag tgttcggcgt gcatcctgtg ctcctcgagc ctctcggttc
    12781 tcacaggcat gaacgtccct accccgacgt gcaacgtgat cctgccgatc ctggctccgg
    12841 cctgctctgc gccttgcaga accctctttg taaagtggaa gccggccgtt ggcgcggcgg
    12901 cggaaccttc gttgcgcgcg tagatggtct ggtagcggct ctccgtttct ggcgttgggc
    12961 ggatgtaagg tgggagcggc atcctgccgc cgcgctccaa cagtccccgc acgttagagg
    13021 cagagacgac ccagtgcccg ttacccaggc tcttcaccaa cctcagccgc accccgcccg
    13081 ctgagagcgt gagaccgggc cgtagacgct tgctcggtct cgccagtact tcccaagcgc
    13141 cgtccagatc cggccccagg tccttgagga agagcagctc gatcgcccct ccggtagact
    13201 tcctggcctc gactcgggcg gggatcactt tcgtctcgtt caggatcagc gcatcgccgg
    13261 gccgcaggaa gtccggcagg tcgctgaagg tatggtgggc tatcgtattg cgacctacgt
    13321 ccaccaccat caggcgggag gcgtcgcgcg gctcggccgg gctctgggcg atcaggtctt
    13381 cgggaagctc gtagtcgagc gcggagacga gcaccttagc ggcggaagag gatattaagg
    13441 acgagggtgc ccacgatgga gagcaggatc atgagcccta tcgggaagta gaaggtggcg
    13501 ttgccgcgcc tgaataccag gtctcccggc agcctgtcta tcccgaggcg cccgagcaat
    13561 aagaacagcc caccgagtat gagcaacact accgcgccgc cgagcaacag ttttcctacg
    13621 ttttctagtc ccacaccact attctattcc ccagacaggg cgtgtccaac ggtggccgcg
    13681 aagttggctt tcggttgata tccatgctac tttacgctcg tagactttcc tgtggggaaa
    13741 tggataacgt agaggagggg taaggagaat gtatgggtag aacgaagtat gcgatcgcct
    13801 tcgctctggc gctggtgatg gctttggggg ctgtgggggt cgcttcggcg gcggtcccgg
    13861 tggattcctc acagctacgc gatgcggtag gggtaggcgg catcctagag cacgagcggg
    13921 cgttccaggc cattgcggac agcaacggcg gtacgcgggc ttcgggaact ccaggttacg
    13981 atgagtcagc ggactacgtc gccgaaaaac ttgagagcgc tggatacagc gtcacacgtc
    14041 aggtattcga ttacgacctg tttatcgaga atagcgaacc ggttctcgac ccgacggacc
    14101 cggatcttcc tgcctacacg ccaggcgaca tgccggacga cgacttctcc acaatgagtt
    14161 actcaggtag cggtgacgtc accgcgccgc ttcaggctgt gggcggcatc atcatcccgt
    14221 cgcccggcgg ctccgcgagc ggctgcaccg cggaggactt tgctggcttc gaggctggta
    14281 acatcgccct gatccagcgc ggaacctgca acttcagaga gaaggtcgag aacgcgcagg
    14341 cggcaggcgc cgtgggtgtc gtaatcttca acgagggcaa cgtagtccct gacgacgacc
    14401 gcgtcggtaa cctcttcggt acgctcgacc cgccgcagat gaaggtaccc gtggtaggaa
    14461 cctcgttcgc tgtcggcgag gaactgtaca accttctgca acaaggccct gttaccttga
    14521 ggctcgtggt cgacgccacg gtcgagcagc ggacgacaga gaacgtgata gccgatacga
    14581 cgacgggacg ggcggatcgc acggtggtcg tcggcgccca cctggactcg gtgcccgaag
    14641 gtccgggcat caacgacaac ggctccggct cagcgacgat cctcgaggtc gcggaggcga
    14701 tgagcgagct cgacatccag ccgcgcaaca gggttcgctt cgcgttctgg ggcgccgagg
    14761 agtccggcct gatcggctcc gagtactacg tcagccagct ccccaagagg gagctcaagg
    14821 acatcgccgt caacctgaac ttcgacatgg ttggatcccc gaactacgtc cgtttcgtct
    14881 acgacggtga cgcctccgac accaactcgc tcggctcgac gggctccggc gtcgtcgagg
    14941 acgtgttcac cgactacttc gcctcacagg gtctcgccag cgagcccacc gcgttcgacg
    15001 ggcgctccga ctacgacgcc tttatcagcg tcggtatccc ggctggcggc ctgttcaccg
    15061 gcgcggagga tatcaagacc gccgaggagg cggaaaccta cggcggcgcc gccgatgtcg
    15121 cctacgaccc ctgctaccac caggcgtgcg acagctttac cccgctcgct gacggcgctg
    15181 accccgccgc ctacgcggct atcgaggcgg gatacccccc cggcacgctc gtcggcaacc
    15241 tcaacaccca ggctctggac gagatgtcgg acgcgaccgc ccacggcacg ctgactttcg
    15301 cccagaccac ctcggcggtc accggcacga ataagggctc gggggccccg atgtcctcag
    15361 ccgccaagta ccacggtccg ctcctgcaaa ggtgatccca caccgggcat agccccgcgg
    15421 acggagaggg agtggcggat aagccactcc ctctctcgtt taacgtgctt cgtccaactc
    15481 gcgaagtaca ccgtccacat ccaaggggcg ggtgaccatc gcgagctccc catcggcggt
    15541 acggggccac tcgtcttgcg ggcgatcgcg atacagctcg acgccgtttc cgtcggggtc
    15601 gcgcaggtac agcgcctcgg agacgccgtg gtccgaggca ccgtcgatag ggtagttcga
    15661 ttccaggagc catttcaagg cctgcgcgag gtctcgacgc tcggggtaga ggatggcgag
    15721 gtggaagagg cctgccgaat gggtcggggc aggacccgcg ttgcggctca tccaggtgtt
    15781 caggccgatg tggtggtgat agccgcccgc agagaggaag accgcgtcgt caccgtacca
    15841 ctgggttacc tcgaagccaa ggatgtccct ataaaagcca agggcccgct cgatgttcgc
    15901 caccttcaaa tgaacgtggc cgatgcttgt cccagggttc actacccgcc tggttcgctc
    15961 gctcgtctcc attcctactc ctagtcctcg atctcactcc ccctgattct accgccgcca
    16021 tccgaagtca tttgcgacca ggaaaaatga tacccccaag ccagcgatga caccgccagc
    16081 gaatctaccg atatccacct accgtggcca cgctttcgac tggaagctca aactacaagc
    16141 tgaaatgccg acaagcgagg gcccgaagcg tgccgacaag gcgcgaagcg ccgggccggc
    16201 gagctgaggc gaagccgaag cgtgctgaca cgctggcacg acgcttctgc gctccctata
    16261 ctcgaggagt cgtcatgtga cccaggagga gaggcaacta tggagatcac agataccagg
    16321 cacaggagcc gcactatact ggaaggacgc gaccgagcgc cggccaggtc cttcctgaag
    16381 ggcatgggct tctctgacga ggacctcagc aagccgatag taggcgtggc ccactcctgg
    16441 atcgagacga tgccgtgcaa ctacaaccac cggcacctgg cggagaaggt aaaagagggc
    16501 atcagggagg ccggcggtac gcccatggag ctcaacacca tctccatctc ggacggcata
    16561 accatgggga cgcaagggat gaagagctcc ctcgtgagcc gggaggtgat cgccgactcc
    16621 atcgagcttg tggggcgcgg ccacatgttc gacgcgatgg taacgatcgt gggctgcgac
    16681 aagaccatcc cggccgccgc catggcccac gcgaggctgg gcataccagg cgccatcgtc
    16741 tattccggct ctatagcacc aggacgcttc aagggtcacg acgtgacgat ccaggacgtc
    16801 ttcgagggtg tcggggccaa cgcggccggc aggatgtctg atgaggacct gctcgacctg
    16861 gaggaacacg cctgtcctgg agcgggagca tgcggcggcc agttcacggc gaacaccatg
    16921 gcgatgatcc tggagtttct cggcctctcg ccgatgggct cggcaggccc tccggccgtc
    16981 gacggccgca aggacgaggt atgcgcccgt gccggcaggc tcgtcatgga gttgctcgag
    17041 cacggtctca caccggagcg catcctcacc cgtgaggcgt tcgagaacgg tat
//
LOCUS       DATMVF010000111         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_340198, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000111 DATMVF010000000
VERSION     DATMVF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340198"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..698)
                     /locus_tag="VN178_05405"
     CDS             complement(321..698)
                     /locus_tag="VN178_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80442.1"
                     /translation="MSMRPLVKNPSVYLCLLVLFIVLFVSTSGCAVSETSSAPKEETS
                     DAPKEAAQQKVDAAQLLADVQKLSDDSMEGRKAGTARQGTTMVLAVSVYKFTRVLEAI
                     SEEKRESVLESMSILEEAMSESE"
     gene            complement(772..>2500)
                     /gene="ggt"
                     /locus_tag="VN178_05410"
     CDS             complement(772..>2500)
                     /gene="ggt"
                     /locus_tag="VN178_05410"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWS80443.1"
                     /translation="TSSEDNVGEETGEETTGPATSTLIAASLDPEPSRSMPSSPAVGA
                     SGMVSSAHPLATQAGLDILAQGGNAFDAAVAVGAAVGVVEPYMSGIGGYGTMVIYDAE
                     KGEMRVLDSRSRAPATLDPDVFRSPTPNYLENRRSAKAASTLGAANVWGALSNDYGEL
                     EWRRLFDPAIELADGGFIIGERTAYYVGKEFSAFPEYAKSFYGNNGAPLGAGDRLVQK
                     DLARSLGLIAEQGAGVVNGGELGQAIDATMRENGGFLTIDDLRNSRAEWRDPISIDYQ
                     GYEVVTASPPIGSWGALLRLGIMSQFDVTAPGHNSADYLHRYAEVTKRAYEARLRYDG
                     DPEVDPAPLDSLLSESYWADKAANINPSRATPFEPPANFSDEGLPKGQEHTTHFVVAD
                     REGNVVSATQTLGNLFGSRVMPRGTGIWINDSLAFAQFEPKGNPLDAFPGRNRLVGVC
                     PTLVMSDGRPWVALGTLGGRTILQTMPQMLMNLIDFRMDIQQAISAPRISFVEPDILA
                     VEGSVPEPVRSELATRGHNISVDELRHGNERGLGNAHGLAIEFDSEGNPVRFTGGADP
                     RGEGVAAGF"
BASE COUNT          414 a          820 c          760 g          506 t
ORIGIN      
        1 tggatctccg acgggtcgga gaggtcgccc cgtcggaacg cggcggcctc ctccttgagg
       61 tgctcgcgga tcagcgcgac ctgctcggcg tcggtcggat cgtcagccac caccttttgc
      121 accccgctag tctgcatctt gtcgaagacg tggtggtttc ctctaggtcg aagggcatca
      181 cctcggcgcc cttctgcgca acctcttcct gccggggctc ctgggaagcg aacgccccca
      241 tcgcccagag catgcccagg acggccaccg tcgctaacgc cgcacccgcc gcgagcagca
      301 gcgccgcccg taccggaagg ttactcgctc tcactcatcg cctcctcaag gatgctcatg
      361 gactccaata ccgactcacg cttctcctcg gagatcgcct ctaggacacg ggtgaacttg
      421 tagaccgaca cagcaaggac catggtggtc ccctgcctcg ccgtgcccgc ctttcggccc
      481 tccatcgaat cgtccgacaa cttctggacg tcggcgagga gctgcgccgc atcgactttc
      541 tgttgcgccg cttccttcgg ggcatcgctc gtctcttcct tcggggcact gctcgtctcc
      601 gaaactgcac accctgaggt ggaaacaaag agcacaataa acagaaccag taggcaaaga
      661 tataccgagg ggtttttaac aagaggtctc atgctcaagg tcgctgcacc taacgaactc
      721 gggcgcatcg gccctgaaaa acctgatgat ggattgcgtt cctggtgcag ctcaaaatcc
      781 ggccgccact ccttcgcctc gcggatccgc tcctcctgta aatcggaccg ggttgccttc
      841 ggaatcgaat tcgatggcca ggccgtgcgc gttgccaagt ccgcgttcgt tgccgtgtct
      901 gagctcgtcc acgctgatgt tgtgacccct ggtcgccagc tcgctgcgaa cgggctccgg
      961 tacgctgccc tcgaccgcga gtatgtctgg ttccacgaaa ctgatgcgcg gggcggagat
     1021 cgcctgctgg atgtccatcc ggaagtcgat caggttcatc agcatctgcg gcatcgtttg
     1081 caggatggtg cggccgccga gggttcctag cgcgacccag ggtctcccgt cgctcatgac
     1141 gagggtcggg cagaccccga cgagcctatt gcggccggga aaggcgtcca gcgggttccc
     1201 tttcggttca aactgggcaa acgcgaggga gtcgttgatc cagatccccg tccctctcgg
     1261 cattaccctg cttccgaaaa ggttgcccag ggtttgcgtc gcggagacta cgttcccctc
     1321 tcgatccgcc acgacgaaat gcgtggtgtg ttcctgcccc ttaggcaggc cctcgtcaga
     1381 gaagtttgcg ggtggttcga acggtgttgc tcgcgaagga tttatgttcg cggctttatc
     1441 ggcccagtaa ctttccgaaa gcagcgagtc cagcggggcc ggatctacct ccggatcgcc
     1501 gtcgtacctg aggcgcgcct cgtatgcccg cttcgtgacc tccgcgtacc tgtgcaggta
     1561 gtcggcgctg ttgtggccgg gcgccgtcac atcgaactgg ctcattatcc ccagccggag
     1621 gagcgcgccc cacgaaccga tcgggggaga cgccgtgacc acctcgtagc cctggtagtc
     1681 gatgcttatg gggtcccgcc attcggcgcg gctgttgcgc agatcgtcga tggtcaagaa
     1741 gccgccgttc tcgcgcatgg tagcgtcgat ggcctggcct aactcgccgc catttaccac
     1801 cccggcgcct tgttcggcga tcaggcctag agacctcgcc aggtcctttt gcacgagacg
     1861 gtcccccgcc cctagcggtg ctccgttgtt cccgtaaaag ctcttcgcgt attcggggaa
     1921 cgcggagaac tctttaccga catagtaggc ggtgcgttcg ccgatgataa atccgccatc
     1981 agcgagttcg atggcggggt cgaagaggcg acgccactca agctcgccgt agtcgtttga
     2041 cagggctccc cacacgtttg ctgcgccaag cgtggaggcg gctttggcgc tccgccggtt
     2101 ttctaagtag tttggcgttg gagaacggaa cacgtccgga tcgagggtcg cgggcgccct
     2161 gctgcgagaa tcaagaaccc gcatctcgcc cttttcggcg tcgtagatga ccatcgtccc
     2221 gtagccgcca atgccggaca tatacggctc cacgacgccc acagcggcgc cgaccgccac
     2281 cgccgcgtcg aacgcgttgc ccccctgggc gaggatgtca agccctgctt gcgtggcaag
     2341 cgggtgggcg gagctgacca taccggaggc cccgacagcc gggctgctcg gcatgcttcg
     2401 gctgggttcc gggtcgagac tagcagcgat gagcgtcgag gtggctggcc cggtggtttc
     2461 ctcgccggtc tcctcgccga cgttgtcctc gctggaagtg
//
LOCUS       DATMVF010000112         9184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_478247, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000112 DATMVF010000000
VERSION     DATMVF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478247"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VN178_05415"
     CDS             complement(<1..170)
                     /locus_tag="VN178_05415"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWS80444.1"
                     /translation="MVLDVPGEPLRAAEVPVPEPGEGQVLLRVHCCAVCRTDLHVVDG
                     ELQDPSLPLVPG"
     gene            complement(234..2069)
                     /gene="glmS"
                     /locus_tag="VN178_05420"
     CDS             complement(234..2069)
                     /gene="glmS"
                     /locus_tag="VN178_05420"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWS80445.1"
                     /translation="MCGIVGYAGGERCVEVLMEGLGHLEYRGYDSAGLALQRAGRIEQ
                     IREVGRLENLTRALAGRNGDLSLVRTGVGHTRWATHGRPSEENAHPHLGGDGGVAVVH
                     NGIIENFSELREELEGRGIIFRSETDTEVMAHMIADRVEAGETLRTALAGVLHRLEGS
                     FAVAAISVDEPDKIVAARHQSPLVVGLGEGENFLASAVHALLGETREFIVIENGEIVE
                     IAGSSVGVFTTSGEPVERESFAVDWDAEAVELGGYEDYMLKEIHEQPAALGATLLGRL
                     DADGAVDLSEVGVDLSEIDRVVIVACGTAYHAGLLGKQAIEKLARIPVEVAIASEYRY
                     ANPIGDDRTLVVAISQSGETTDTLAAVQAARGFGARVLAVTNTRGSLITREADAVLLT
                     KAGPEICVASTKAFVAQVAVLDLLALALAGIREAVPERELREIGRDLRLAPEKVEETL
                     GLLGNRVEEAVDIFEDARCSLFLGRGISYPVALEGALKLKEISYLPSEGYPAGEMKHG
                     PIALVDEHCPVVAVLGEGLIREKTLSNVEETVARGAGVIVVAREGDKGAQRMARLLLP
                     VPEAPEILAPFICSVPLQLLAYHVAKNRGLDVDKPRNLAKSVTVE"
     gene            complement(2450..3187)
                     /locus_tag="VN178_05425"
     CDS             complement(2450..3187)
                     /locus_tag="VN178_05425"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HWS80446.1"
                     /translation="MNRFWEVDAARGVAIIMMIVYHTTYDLDTLGGYDVQSTTGYWAL
                     FADLTAGLFLLLVGVSLAISRARTSLMGWRLFGKYLARGLRILAYGVVLTLVFLALGM
                     GVVAFGILHLIGISIILAYPFLRLRLTNLVLGTLIFVVGQYILAQGLDSQSFWLLPFG
                     IVPEGVIMPDYRPLLPWFGVVLIGLFFGNVVYGDGRRPAALGDKAPMLARPLPPPGRN
                     SLFIYLIHQPIIIALLTVTGVVDLNFS"
     gene            3272..3751
                     /locus_tag="VN178_05430"
     CDS             3272..3751
                     /locus_tag="VN178_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80447.1"
                     /translation="MNEDFTIRPGRKEDAAETARLWMRSAEEHTAHDRVYQTAPGAEK
                     TMRRFLADVANSGYSFLFVVAAGDRTVGFISGELRQGSPTFLPKTWASVDDVFVEPEY
                     RNRGMGRALLQSVQSWAKERGADGISLQVAAANSRGRKFYEDLGFREVSVYQVFEFD"
     gene            complement(3908..4372)
                     /locus_tag="VN178_05435"
     CDS             complement(3908..4372)
                     /locus_tag="VN178_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80448.1"
                     /translation="MRPVEVARVDATDLAESLVLLEEAVRDGDPISKDFADRLLKAIE
                     AGDVEVLAAWAENQIMGVVLLAYRLSVSAGGLFASVEDLYVRPVARRQGIGRALLEAV
                     DELCAERDISYVEVQVEEEVAEQFYAALGYEPELSVRVLSRSLPINERKTKS"
     gene            complement(4369..5388)
                     /gene="ltaE"
                     /locus_tag="VN178_05440"
     CDS             complement(4369..5388)
                     /gene="ltaE"
                     /locus_tag="VN178_05440"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HWS80449.1"
                     /translation="MIDLRSDTVTRPTEGMRRAMLEAPVGDDVFAEDPTVNRLEEYVA
                     DLLGKEAALYAPSGTMTNQIGVQVNTNRGEEVLLHEGSHIFVYEAGAPAMLSSVQLRI
                     LPGENGVIDPETVRAAVRSEDVHFPRSRLLCLENTHNTSGGRVFPLEDFATAAETAKE
                     LGLKVHLDGARLFNAQAATGVPAREWCAHADTVSVCSSKGLGAPVGSLLAGEAETIRE
                     ARRARKAFGGGMRQAGIIAAAALYAFENHVDRLGEDHEHASHLASGLREAGYEAKEPE
                     TNLVLVAVDDPPEFLRALAREGVLATPGKPGYVRLCTHLDVGEREIEAAIQAAARIAP
                     SAIER"
     gene            complement(5385..6314)
                     /locus_tag="VN178_05445"
     CDS             complement(5385..6314)
                     /locus_tag="VN178_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80450.1"
                     /translation="MNPPTEVPPELRALLDNEGVEIAGSRTIDHGTQYDLARDGETAK
                     LNVYRTGKISAGGRASRLRELLEAWRTSQASVGGTRTPSARRPLLDGTPRLGIDEAGK
                     GDYFGPLVVAGVRITNAETASKLQGLGIRDSKEIRASGIRRISGRIVDAVGRNNVHVI
                     SLPPQEYESRRDAAGNVNKLLGELDATIVGKLVDEVEIVVVDEFARSARSYLEPFVPG
                     GVRLEVRPRAEDDAAVAAASIVARARQLEEIERLSERVGFGLPLGATHVLEAGRRVVR
                     ELGEHGLAEVAKVHFSTTRRVLQTAGQSDGGNP"
     gene            complement(6311..6595)
                     /locus_tag="VN178_05450"
     CDS             complement(6311..6595)
                     /locus_tag="VN178_05450"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWS80451.1"
                     /translation="MQNSDLQRAHVLVSGQVQGVFFRDSTRQKAEELGLAGWVKNTPD
                     GQVEALFEGPSERVKEMVRWCEEGPQRASVENVDTNFESSDGDLEGFEVR"
     gene            6672..8228
                     /locus_tag="VN178_05455"
     CDS             6672..8228
                     /locus_tag="VN178_05455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWS80452.1"
                     /translation="MKAEKQAVTGEAEPGRDQEKVLLTLVVPTRNEAENVPKLVHELR
                     ESLSDLDYRVVFVDDSTDETPEVIQGLSEHDDRIRLVRREGAERYGGLSTAVTTGIEL
                     FSSASEYTCVMDADLQHPPEKVREMLEIARRSDADVVVASRYGPGGGYAGLTGKMRRA
                     VSVGSKYLARLLFKEARKTNDPMAGFFLVKNQAISGIQFRPTGFKVLLEILVCAPELK
                     VLETPFEFQVRHAGVSKATMRQGFEYLTHILSLFWYVPSAGRFWKFALVGASGVLVNM
                     VTLIVLAEYFHAHKVIAWMFAVGLSILSNFLLNNAFTWRDIRHSSRIHFFLRGVLAYP
                     VAVMGIGANFAVWYPLVKYVSDDFPYYALFNLLGIVAGTSVNFILSSQLVFRPSTPKN
                     LDPDAPKELIAEMLRRELKADRVALIRVPDLTPLGEHSPRDLTASDRSVIDLVVKTTH
                     PTLTVTGPRRLPQARTNARWTNSLAVPIINDDHALGVVYATRKSTEPFTEEDLNWLTA
                     YTSTAARIFE"
     gene            8955..>9184
                     /locus_tag="VN178_05460"
     CDS             8955..>9184
                     /locus_tag="VN178_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWS80453.1"
                     /translation="MRPICFLSDFGLSDDFVGTCKGVMLRIAPGVTIVDLTHEVPGFE
                     VEAGAEILQHATRYMPADTVYLAVVDPGVGTKR"
BASE COUNT         1669 a         3041 c         2759 g         1715 t
ORIGIN      
        1 tgtcctggga ccaggggcag actgggatcc tgcagctccc cgtcgacgac gtgaagatca
       61 gtcctgcaca cggcgcagca atgtacgcgc aataggacct gtccctctcc cggctcgggc
      121 acaggcacct ccgcggcccg gagcggctcg ccgggtacgt ccaggaccat cgctcgcaca
      181 gaccgatcct cctatcctcc tacattggga gcacaaactg ctttggggac acgctactct
      241 accgtgacgc tcttggcgag gttgcgtggc ttgtcgacgt cgaggcccct gttcttggcg
      301 acgtggtatg cgaggagttg cagcggcacg ctacagatga agggagccag gatctccggc
      361 gcctcgggga cggggagcag caggcgcgcc atcctctgtg cgcctttgtc accctcacgg
      421 gcgacgacga tcacgccggc gcccctggcg acggtctcct cgacgttgga gagggtcttc
      481 tcgcggatga ggccctctcc gagaacggct acgacgggac agtgttcgtc caccagggct
      541 atgggaccgt gcttcatctc gcccgccgga tagccctcgg agggcaggta ggagatctcc
      601 ttgagcttca aagccccttc gagcgccacg ggatacgaga taccgcgccc caggaagagc
      661 gagcaccgcg catcctcgaa gatatcgacg gcctcctcca cccggttccc gagcaacccg
      721 agcgtctcct cgaccttctc cggcgccagc ctgaggtcgc gaccgatctc ccgcaattcc
      781 ctctcgggta cggcctcgcg gatgccggcc agcgccagcg ccagcaggtc gagtacggcg
      841 acctgggcga cgaaggcttt cgtcgaggcg acgcagatct cgggccccgc cttcgtgagc
      901 aagaccgcgt cggcctcgcg ggtaatgagc gaccccctgg tattggtaac cgcaagcacc
      961 ctggcgccga agccgcgcgc cgcctgaacg gccgccaggg tatccgtcgt ctccccactc
     1021 tggcttatgg cgacgaccag cgtgcggtcg tctccgatag ggttcgcata acgatactcg
     1081 ctggcgattg cgacctcgac gggtatcctg gcgagcttct ctatggcttg tttgcccaga
     1141 aggcctgcgt gataggccgt gccacaggcg acgatcacga cccggtctat ctcggagagg
     1201 tcgaccccca cctcggagag gtcgacggcg ccgtctgcat cgaggcggcc taggagcgtc
     1261 gcaccgaggg cggccggctg ctcgtggatc tccttgagca tgtagtcctc gtagccgccg
     1321 agttcgacgg cctcggcgtc ccagtccacc gcgaaggact cgcgctcgac gggctcgccc
     1381 gaagtcgtga agacccccac cgaagagccg gctatctcga cgatctcgcc gttctcgatg
     1441 actatgaact cgcgtgtctc tccgagcagc gcatgcaccg cgctggccag gaaattctca
     1501 ccttccccca ggccgacgac gagcgggctc tggtggcggg ccgcgacgat cttatcgggc
     1561 tcatccacgc tgattgcggc gacggcgaag gaaccttcta gccggtgcag caccccggcg
     1621 agcgccgtcc gtaacgtctc cccggcctcg accctatcgg cgatcatgtg cgccatcacc
     1681 tctgtgtccg tctcggaacg gaagatgatg ccccgcccct ccaactcctc cctgagttcc
     1741 gagaagttct cgatgatgcc gttgtgtacg acggccacgc ccccatctcc gccgaggtgg
     1801 ggatgcgcgt tctcctcgct ggggcgtccg tgcgtcgccc agcgggtgtg tcccaccccg
     1861 gtccgcacca gggagaggtc accgttgcgt ccggcaagag ctcgggtgag gttctcgagt
     1921 cgccccacct ctcttatctg ctctatccgg ccagcccttt gaagggccag acccgccgaa
     1981 tcgtaacccc gatactcgag gtgcccgaga ccttccatga gtacctcgac acaccgctcc
     2041 ccaccggcat aacccactat gccacacatg gacttacctc ccttgtcttc cgaagatggg
     2101 atcagggagg gcagaggaaa gagacagagc atctagccct gtctccgggc ccggacggtg
     2161 ctttgtcccc ggcgtcacca ccgggatttg tcaccgccct gacgaccgcc cgaagccgag
     2221 acgtacccgc cgaatgcttc gatgaacgtc ttcctcgtcg actcccgcac agtgggagtc
     2281 ctggcgcttc tatttaccct ctgtcccgca agctccgacg atcccatcgc gctcgctggt
     2341 catctactgc gaatacgatc ttctccagtc cacctccata tcctctatgg aacggcacag
     2401 ctaactgtaa cggttacgcc gcgaaaaaga aaccggccgc cctgaagctc tacgaaaagt
     2461 tcaggtccac gacgcccgtt acggtgagca aggcgataat gataggctgg tggatcaggt
     2521 agatgaacaa ggagtttctg cctggcgggg gaagcggtct cgccaacatc ggcgccttgt
     2581 cccccagggc cgcgggcctt ctgccgtcac cgtacaccac gttcccgaag aacagcccga
     2641 taaggacgac accgaaccag ggcagcaggg gcctgtaatc cggcattatc acgccctctg
     2701 gtacgatgcc gaaaggcaag agccagaagc tctgcgagtc gaggccctgg gccaggatgt
     2761 actggccgac gacgaatatg agagtgccca gcacgagatt ggtcaaccgg agcctcagaa
     2821 aggggtaggc gaggattatg gagatcccga tcaggtgcag tatgccgaac gccacaacac
     2881 ccatcccaag ggccaggaag acgagggtca acaccactcc gtacgccagg atcctcaagc
     2941 ccctcgccag gtactttccg aagagacgcc agcccatcag gctcgtcctc gcccggctaa
     3001 tggcgagcga gacgccgaca agtaacagaa agaggcctgc cgtcaagtcg gcgaagagcg
     3061 cccagtaccc cgtagtggac tggacgtcgt atccgcctag ggtgtccagg tcgtaagtgg
     3121 tgtggtagac gatcatcatg atgatggcga cgccgcgggc cgcatccacc tcccagaacc
     3181 tgttcacttt cctctccgta ccccgctcct ctactatttt tccggatcat accccacccc
     3241 cggcccccga ggcatgggtt aggattgctt tgtgaacgag gacttcacca tcaggccggg
     3301 gcgcaaagag gacgctgccg agacggcgcg gctgtggatg cggagcgccg aggagcacac
     3361 cgcccacgac agggtctacc agacagcccc aggcgccgag aagacgatgc ggcgctttct
     3421 ggccgacgtg gcgaacagtg gctactcctt tctcttcgta gtcgcagcgg gagatcggac
     3481 ggtcggcttt atctcgggcg agctacgtca gggctcgccg accttcctcc cgaagacgtg
     3541 ggcctcagta gatgacgtct tcgtcgaacc cgagtacagg aaccgcggta tgggccgcgc
     3601 cctcctccag agcgtccagt cctgggcgaa ggagaggggc gcggacggta tctcactcca
     3661 ggtcgccgcc gcgaactcgc ggggccgcaa gttctacgaa gacctgggat ttcgggaagt
     3721 ctcggtgtac caggtgttcg agtttgatta gaggtttcag gcttcaggta tgggcttttc
     3781 tagctgagag ttacagcctt ctctctggcc agcaccgccg cttcttccgt cattcccgcg
     3841 aaagcgggaa tccacttcac cacgtgttgt gctcctcaag ccggaagtga acttccggca
     3901 tcagccgtta gctcttggtc tttctctcgt tgatgggtag ggagcgggag aggactcgta
     3961 cactcaactc cggctcgtaa cccaaggcgg cgtagaactg ttcggccact tcttcttcga
     4021 cctgtacttc aacgtaagag atgtcgcgct ccgcgcaaag ttcgtctacg gcctctaaca
     4081 gcgcccggcc gatgccctgg cgtctggcta cggggcgcac gtagagatct tcgacgctgg
     4141 cgaagagtcc gccggcggag acgctgagac ggtaggcgag caagaccacg cccatgatct
     4201 ggttctccgc ccaggcggcc agtacctcca cgtctccggc ctcgatggcc tttaggaggc
     4261 ggtcggcgaa gtctttggat atgggatctc catccctgac ggcttcttcg aggagtacca
     4321 gggattcggc aaggtcggtt gcgtcgaccc gtgcgacctc gaccggcctc aacgttcgat
     4381 cgcgctgggg gcgatcctgg ccgccgcctg gatggcggcc tctatctccc tttccccgac
     4441 gtcgaggtgc gtgcacagac ggacgtagcc gggtttgccg ggggtggcga gcacgccttc
     4501 acgggcgagg gcccgcagga attcgggagg atcgtcgacg gcgacgagga cgaggttggt
     4561 ctcgggctct ttggcctcgt agccggcctc tctcagtcct gaggccaggt ggctggcgtg
     4621 ttcgtgatcc tcgccgagcc tgtctacgtg gttctcgaag gcgtagaggg ccgccgcggc
     4681 gatgatgccg gcctgcctca taccgccgcc gaaggccttg cgcgcccgac gtgcctccct
     4741 gatggtctcc gcctcaccgg cgagcaggga cccaacaggc gccccgagcc ccttggagga
     4801 gcaaacggag accgtgtcgg cgtgcgcgca ccactccctg gcggggacgc cggtcgcggc
     4861 ctgcgcgttg aagagcctgg ccccgtcgag gtgcaccttc aggccgagtt ccttcgccgt
     4921 ctcggcggcc gtggcgaagt cctcgagtgg gaagacccta ccgcccgaag tgttgtgggt
     4981 gttctccagg cagagcagcc tggagcgcgg gaagtggacg tcctcggacc tgacggcggc
     5041 gcgcacggtc tcagggtcga tgacgccgtt ctctccgggg aggatgcgga gttggacgct
     5101 ggagagcatc gccggcgccc cagcctcgta gacgaagatg tgggaacctt cgtgcaggag
     5161 cacctcttct ccgcggttcg tgttgacctg gaccccgatc tggttggtca tcgttcctga
     5221 aggcgcgtat agcgccgcct ccttgcccaa caggtcagcc acgtactcct cgagccggtt
     5281 caccgtcggg tcctccgcga aaacgtcgtc cccgaccggg gcctcgagca tcgcccgccg
     5341 catgccctcc gtgggacggg tcaccgtgtc actgcgaaga tctatcaagg gttgcctcca
     5401 tcgctctgtc ccgccgtctg cagcacccgg cgcgtggtgg agaagtgaac cttcgccacc
     5461 tccgccagac cgtgttcccc gagttcccta accacccgcc gacctgcctc caaaacgtgc
     5521 gtcgccccga gcggcaatcc gaaccctacc cgctccgaga gacgctcgat ctcctcgagt
     5581 tgccgcgccc gcgccactat cgatgcggca gccaccgccg cgtcgtcctc ggcccgaggg
     5641 cgcacctcca gccgcacgcc tccgggcacg aacggctcga gataggagcg ggcggaacgg
     5701 gcaaactcgt ccacgactac tatttctacc tcatccacga gtttacccac gatcgttgcg
     5761 tcgagctcgc cgagcagttt gttgacgttt ccggcggcgt ccctgcggga ctcgtactcc
     5821 tgaggcggga gcgaaattac gtggacgttg ttccggccta cggcatccac gatgcgtccg
     5881 gagatccggc gtatgccgga agcccggatc tccttcgaat ccctaatccc tagcccctgc
     5941 agcttactcg cagtctctgc gtttgtaatc cggactccgg cgacgaccag agggccgaag
     6001 taatcgccct tcccggcctc gtcgataccg agcctcggcg tgccgtcgag aagaggtctc
     6061 cgggctgacg gagtccgagt accacctacg ctggcctgcg acgtgcgcca ggcttcgagc
     6121 agctcccgca gcctcgaggc cctacccccg gcagagatct tgcccgtacg atagacgttc
     6181 agcttcgcgg tctcaccgtc gcgggccagg tcgtactggg tcccgtgatc tatggtcctg
     6241 ctgcccgcta tctcgacacc ttcattgtcg agaagcgccc gcagctctgg ggggacttcg
     6301 gtgggtgggt tcaacggacc tcgaagcctt cgaggtctcc gtcggagctc tcgaagtttg
     6361 tatccacgtt ctccaccgag gcgcgctgag ggccttcttc gcaccagcgg accatctctt
     6421 tgaccctttc ggaggggcct tcgaacaggg cttcgacctg cccgtcgggg gtgttcttta
     6481 cccatccggc caggccgagt tcttcggcct tctgcctggt cgagtccctg aagaagacac
     6541 cctgcacctg gcccgagacc aggacgtgcg cgcgctgtag atcgctgttc tgcacttttc
     6601 gtacctccgt ctaccgtatt atcgtccgac gtgttgggca ggttaaccca atatgctagc
     6661 ctagagcggc catgaaagca gagaagcaag cggttaccgg ggaggcggaa cctgggagag
     6721 accaggagaa ggtactcctt accctggtcg tgccgacccg caacgaggcc gagaacgtcc
     6781 cgaagctcgt gcacgagttg cgcgagagcc tgtcggacct ggattacagg gtggtcttcg
     6841 tggacgactc gacggacgaa acgccggagg tgatccaggg cctctcagaa cacgacgacc
     6901 gcatccggct ggtgcgccgg gaaggggcgg agcggtacgg tggcctctcg acggccgtga
     6961 cgacgggaat cgagctgttc tctagcgcga gcgagtacac ctgcgtgatg gacgcggact
     7021 tgcagcaccc tcccgagaag gtgcgcgaga tgctggagat cgcacgccgc tccgacgccg
     7081 acgtcgtcgt ggcgagccgc tacggccctg gcggcggcta cgcaggcctc accggcaaga
     7141 tgcgtcgggc cgtgtccgtg ggaagcaaat acctggcccg gctcctcttc aaggaggccc
     7201 gcaagacgaa cgacccgatg gcgggcttct tcctcgttaa gaaccaggcg atctcgggca
     7261 tccagttcag gccgacgggg ttcaaggtgc tgctcgagat actcgtgtgc gcgcccgagc
     7321 tcaaggtgct cgaaacgcct ttcgagttcc aggttcgcca cgccggggta tccaaggcga
     7381 caatgcgcca gggcttcgag tacctgaccc acatactgag cctgttctgg tacgtgcctt
     7441 cggcagggcg cttctggaag ttcgccctcg tcggggcctc aggcgtgctg gtcaacatgg
     7501 tgacgctcat cgtgctcgcc gagtacttcc acgcccacaa ggtcatagcc tggatgttcg
     7561 cggtcgggct ctccatcctc agcaacttcc tcctcaacaa cgcgttcacc tggcgcgata
     7621 tcaggcactc cagcaggatc cacttcttcc tgcgcggcgt cctggcctat cccgtcgccg
     7681 tgatgggcat cggggcgaac ttcgccgtct ggtatccgct cgtgaagtac gtctcagatg
     7741 actttccgta ctacgcgctg ttcaacctgc tcggcatcgt ggcggggacg agcgtgaact
     7801 tcatcctctc ctcgcagctc gtgttccgtc cctcgacgcc aaagaacctc gaccccgatg
     7861 ccccaaagga gctgatagcc gagatgctaa gacgcgaact caaagccgac agggtagcct
     7921 tgatcagggt tcccgatctc acgcctctcg gagaacattc gcccagggat ctaaccgcct
     7981 cggacaggag cgtcatagac ctggtcgtca agaccaccca ccccaccctg accgtgaccg
     8041 gcccccgacg cctgccccag gcccgcacga acgcccgctg gaccaactcc ctggcagtcc
     8101 cgatcatcaa cgacgatcac gcccttggcg tagtctacgc cacccggaaa tcgaccgaac
     8161 cctttaccga agaagatctc aactggctga ccgcctacac cagcaccgca gccaggatat
     8221 tcgagtaacg tcagcacggc gcttcgcgcc ttgtcagcac gctcaggctt acgccttcgc
     8281 tcgtcagcat gtcagctaaa ggcttctcag gatgatcccg taccccaaca accgaacggg
     8341 catatttagc cctcgtttac gcgaaagcgc aaggttccgt attcagctga ctagctgaat
     8401 tgctgacatg ctgccgcagg cagcgtgctg aagtgctgac gaggcggcaa agccgccggg
     8461 ctgaattgcg cgggcgggga agtcaaacct cccccgcccg caacatcggc cccccccaac
     8521 aggagcctct agccggactc gatcgcccct cccacagatc aagtccgccg cgatcccacg
     8581 cctagaccca agcttcaaac cgagccccgg gtaaacggga atcaaccaga tagaaactag
     8641 catcctccta cacatcccgc aacctcttgc aatccctata cctaagccag gacatgtata
     8701 ccatgtgtcc tcaggtacga catatccgta accctccacc aatcaacaca atatgttgag
     8761 tttttaccaa aatcataatc tttcataaca tgtctattaa cgtgttagta gagggtcagg
     8821 taagtttaat gttgagatgc atccattggt gctggcgggg cccggtatag tcttggggcg
     8881 ggttgtggat gtcggcctgg gcggctctga gggtcgctac gacgggctgg ggtggtacgc
     8941 tttatactag gggcatgagg ccgatttgct ttctgtccga cttcggtcta tccgacgact
     9001 tcgtcggcac ctgcaagggt gtgatgttga ggatcgcgcc tggagtgacg atcgtcgatc
     9061 tgacgcacga agtacccggt ttcgaggtgg aggcgggggc tgagatcctg cagcacgcca
     9121 cccgctacat gcccgctgat acggtatacc tggcggtggt tgatccggga gtcggcacaa
     9181 agcg
//
LOCUS       DATMVF010000113        23331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_340488, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000113 DATMVF010000000
VERSION     DATMVF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 23331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23331
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340488"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VN178_05465"
     CDS             <1..315
                     /locus_tag="VN178_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS80454.1"
                     /translation="YDEYAEARRDDLRKEAQEILDEQVRKIEEAGGTVAVAHVKMDEQ
                     RDEAIVHLAEDIDAGMIVIGSRGFGGMRRALMGNVADSVVRHAHCPVLVVRPTGGISR
                     PF"
     gene            complement(673..1668)
                     /locus_tag="VN178_05470"
     CDS             complement(673..1668)
                     /locus_tag="VN178_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HWS80455.1"
                     /translation="MNQERFDDLTRALATGRISRRQVLKAAAVAAVAAGTLLKQRQAE
                     AARQAADLERFYAGIKSDTQVEHEFGGMKAGILTPEGFRAQQHAERTVDEATQRDLLD
                     VVYGIDVSHWQGELDWHRIWQEEFRFCGIKATEGPYPNRKRYTDPYYAINARRSKEAG
                     FVRFPYHFLVNAPIREQVAHFLHVAGAGGWGQGQCPLIDFEAYPDFPSLSPKPEHLYG
                     FIQQLRERIGREPLIFVYAGKSYTDSLGGIDLSTFDNVCLWDAAYYLGSKRGLASVLW
                     HEIVNAGYHPFAKARWGNRSKKVSQFTSTAKIAGKLMDADAWHGDLYDLRHATGW"
     gene            complement(2188..2961)
                     /gene="fetB"
                     /locus_tag="VN178_05475"
     CDS             complement(2188..2961)
                     /gene="fetB"
                     /locus_tag="VN178_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HWS80456.1"
                     /translation="MADLQTLGSALLTLGLVAIAIVLSLYERLDLEKDIGVAVVRSFL
                     QLAAIGYVINFIFGLKSIGAVVLLLAGMVLFAAWTSASRAEDVPRAFLIAAVAVGAAA
                     VATLGLLLTLRIVPPTARYLIPLGGMVIGNSMNAASLMMTRIRDDVAEQMPKVEAALA
                     LGATSRQAIAPILRTALQRALIPLIDATKTTGIIFLPGAMVGMIIAGADPLEAVRLQI
                     VVLYMLLGSVSIAAILVGTLSYRSFFTPRHQLRTTPPER"
     gene            complement(2963..3673)
                     /locus_tag="VN178_05480"
     CDS             complement(2963..3673)
                     /locus_tag="VN178_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS80457.1"
                     /translation="MDRPKIEVRGLRYVVGGVEILKGVDAGMPDGEITAVVGPSGAGK
                     STLLRAINRLIEPSSGEIYLSGEPTSTLDPSELRRRVGMVFQVPALFGDSVEEAVLYG
                     ARLLGRDADPRRLLGMVGLDASLAGRDPQSLSVGQQQRATIARALALAPEALLLDEPT
                     SALDEAARTRIEDLVRDLNARLGLTMVFVSHDLAQVERVADRVLVLAEGKSVGVWDRG
                     DFFSGAGERARQFIAGRL"
     gene            complement(3675..4091)
                     /locus_tag="VN178_05485"
     CDS             complement(3675..4091)
                     /locus_tag="VN178_05485"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HWS80458.1"
                     /translation="MLWATRRGCHVDRTACIWLIRRFLDPEADFSFFGDPAEAPEGAE
                     LFDVVGARLSHRGEDCSFETFLKEYGLEDPVLHEIAEIVHDADLVDEKYGRPESEGLD
                     AIVRGMQLALPDDHTLAEHTDTLYDGLYAYLRRETL"
     gene            complement(4091..4606)
                     /locus_tag="VN178_05490"
     CDS             complement(4091..4606)
                     /locus_tag="VN178_05490"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HWS80459.1"
                     /translation="MERTRWVVLVYRLPREPSRHRVAVWRKLRDLGALYLQDGVAVLP
                     EDAVTREQLEWLQLRVREAGGDATLWEARTGTVAEEAGLVEDFRHSREEAYRAIIAEA
                     GRLRRKARMGGGGVLEQLGKLERRFRAERRRDFFRSPLRGEAAEALKATREAARAGQE
                     SAGTLGGEGKR"
     gene            complement(4640..4984)
                     /locus_tag="VN178_05495"
     CDS             complement(4640..4984)
                     /locus_tag="VN178_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWS80460.1"
                     /translation="MTQRNVNARTLRGMLQRGERIALVDVRKREEYAQGSIPGSVNFD
                     AYDALKAGDGQAMEEFELPEGTRVVTFCNRGRAGAVAAEQLRQRGYEAFSLEGGLEAW
                     EGALRRARAWPA"
     gene            complement(5014..6348)
                     /gene="chrA"
                     /locus_tag="VN178_05500"
     CDS             complement(5014..6348)
                     /gene="chrA"
                     /locus_tag="VN178_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HWS80461.1"
                     /translation="MENEKAARGGTVTKPVGFWEAFWFWVKLGFINFGGPAGQIAIMH
                     RELVERKRWVSEKQYLRTLNFCMLLPGPEAQQVATYIGWRLHGTLGGVVAGSFFVIPS
                     IFVLLLLSYLAVAHSDVPAITGLLYGIQPVVIAIVVEAVLRIGKRTINHRMPVGFAIV
                     AFVALYFLSVPFPLVVLAAAVAGLLLSRTGFAGDAFAGGGQDSSEEEESAIDRTDPNG
                     GPSLLRNARLLGTFVLLWALPLGALYLWRGGDDVLIKEALFFTGAAFLTFGGAYSVLS
                     YVSDVAVNHYGWLTADQMVQGLGLAESTPGPLIMVTQYVGFIGAWNDPGPFSPLLYGT
                     LGALITTYATFLPCFLFIFFLAPYIELLANDRRLKAILIGVTAAVVGVIANLAVYFAT
                     QVLFPDGFSLDGLDVFALVVAVVSFVVLQRYKVPIYLMVPAGAVLGMVWTLL"
     gene            complement(6429..7286)
                     /locus_tag="VN178_05505"
     CDS             complement(6429..7286)
                     /locus_tag="VN178_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80462.1"
                     /translation="MLEFLSSATSVAPLGAYWHPGDVIWGMYQNTVFDARQEVRFWEE
                     GGELVGFALLEEPDGVMMQVHPRLRGSGSLEMEMLEWAAGQTRAVYGERAGDELWTRV
                     AEDDPRLDALLAGLGFGRDPDHALKMVRTLDEPLPGHALPDGWTVREVGGEGEWRARV
                     EVHREVWHPSRVTLEAYRRLRAAPGYNPYLDLVVVAPDGEFGSYGICWFDPKSLTGLF
                     EPLGTRQTHRGKGLGKALMQEGLRRLRAIGAQTAFVSAIHDNEAARSLYESMDFYTIN
                     RERLYGKKR"
     gene            complement(7642..8106)
                     /locus_tag="VN178_05510"
     CDS             complement(7642..8106)
                     /locus_tag="VN178_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWS80463.1"
                     /translation="MQVSAKTDYALRAASELALAATEDAGPIKGDWISETQGIPKKFM
                     ENILHDLKRSGIVRTRRGASGGYWLALPAEEISLAAIIRAVEGPLANVRGEWPEAVEY
                     QGAAEHLQEVWIAVRANLRAVLESVTLADIVDGKLPSSITKLTSDPEAWVHH"
     gene            complement(8145..8267)
                     /locus_tag="VN178_05515"
     CDS             complement(8145..8267)
                     /locus_tag="VN178_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80464.1"
                     /translation="MATEYLQFAGALVFFLGILIGSYWFVYVPEQGERKGDDER"
     gene            complement(8386..8598)
                     /locus_tag="VN178_05520"
     CDS             complement(8386..8598)
                     /locus_tag="VN178_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80465.1"
                     /translation="MKGGQDWSRVEEERFEVVAVTLFGKIVVARYATLEQAEWRASEL
                     GEEAERNPRGYVQYLARPAQRPARER"
     gene            8699..10321
                     /locus_tag="VN178_05525"
     CDS             8699..10321
                     /locus_tag="VN178_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWS80466.1"
                     /translation="MLEGLVQDFQLTLPYFLRRAEQLYGHREIVTRLPDESFHRHTYA
                     DFVSRSKKLAVALGNLGLEPGDRVGTLGWNTYQHLEAYLGVPSSGLVLHTLNPRLHED
                     DLAYIAGHADDRVMLVDETLVPVFEKFRARTNVEHAVVMTEGGDVPDGMVSYEDLIGD
                     SDEADFEYPDFDENQAAAMCYTSGTTGRPKGALYSHRSTVLHSMMAAMGNTFALSEAD
                     CVLPVVPMFHVNAWGLPFASVLVGAKLMMPGPHLDAASLLEDLEQEEVTLTAGVPTVF
                     LAVLQALDAEPERYDLSKLRAMVIGGSAAPKGVIKDYKRHGIEVVHAWGMTEMSPIGT
                     VANLTTEMKKLPEEEQLEYEAKQGYPVPFVEIRARGDEGLIPWDGESMGELEVRGPWV
                     ASSYYEAPEGDDKFTDDGWFRTGDVVTIDRFGFIKITDRSKDLIKSGGEWISSVELEN
                     ALMAHPAVSQACVIAIPHEKWDERPLAAIVLREGESATEDELHTHLEVDFASFWLPDA
                     YEFVEAIPMTATGKFQKLKLREQFEGYTSPKS"
     gene            10323..11102
                     /locus_tag="VN178_05530"
     CDS             10323..11102
                     /locus_tag="VN178_05530"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS80467.1"
                     /translation="MKVIGAGFGRTGTMSLKVALETLGFDPCYHMTEVFAHPEHAGFW
                     ISAWRGEPADWDRVLGEYEAAVDWPACTFYEELMERDPGAKVILSVRDPERWYESVRN
                     TIYELSVVIPRHPIYRIGHKLVSFFVFRGPGIVDLASEIIWRGTFDGRFEDKTYATGV
                     FERHSAEVQRRVPENRLLVYDVKAGWEPLCEFLGVEVPDEPFPRTNDTAQMRRRQRGV
                     KALSFVAPVALLVLLFALLVLIVRGTGRSPLNSLPARRLRS"
     gene            complement(11068..12327)
                     /locus_tag="VN178_05535"
     CDS             complement(11068..12327)
                     /locus_tag="VN178_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80468.1"
                     /translation="MVAGKSRWWKSTAFVVGCGCLIALITYGLRTSFGLFATPISEGR
                     GWSPEVFALAIAIQNIVWGLGQPFAGAVADRYGSARILAAGGVVYAAGVALMALSPTP
                     LAFNLTAGVLVGLGLSGASFTIVIAALGRLVPPDRSSQAMGLATAAGSLGQFVFAPLG
                     QAFLADYGWQAALLLLAGCMVVVPALAAALKGGGVGRQGMKEPALPARDAVRQAFGHG
                     SYLLLVAGFFVCGFHVAFITTHLPGHITSVAGHSHGGMHTAGPAVAAWALALIGLFNI
                     VGSYGSGVLGARHSKRRLLAGIYLARAVVIALFTSLPPTPAVVLAFAAAIGVLWLSTV
                     PLTSGLVAVMFGTRYLGTLFGIVFLSHQVGAFLGVWLGGLAFEQTGSFMPIWWAGIVL
                     AIVAAGLHWPIVERPAPSFAASAQAGS"
     gene            12542..12742
                     /locus_tag="VN178_05540"
     CDS             12542..12742
                     /locus_tag="VN178_05540"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HWS80469.1"
                     /translation="MWAKGITFLPGHRMRVEITSSSHPRWERNLNTGESTLESSQTEV
                     AHQIIFHDAGRWSRATLTVVER"
     gene            13025..13609
                     /locus_tag="VN178_05545"
     CDS             13025..13609
                     /locus_tag="VN178_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS80470.1"
                     /translation="MPMEKVRRQFETNVFGLARMCQLVLPGMRRQGYGRIVNVSSMGG
                     RLTFPGAGYYHATKHAVEALSDALRFEVQGFGVDVSVIEPGLIMTGFAETAVGSMDGS
                     DGEDPYTGFDEAVARVTAENYERGLISRLGGGPEAVAEAIEHALSARSPRSRYAVTPS
                     AHLFMRLRRLLPDRVWDALLRAGPYYQPGRKPRR"
     gene            13836..14285
                     /locus_tag="VN178_05550"
     CDS             13836..14285
                     /locus_tag="VN178_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS80471.1"
                     /translation="MEQSMDALSQVIKSERAIRGWSQGELAERSGVSRKTISDIENGS
                     LKRAPQLRTVHKLLWVLPTEAPPSGAKPPQEAWEEIKGFVDLLNKTFEGGTTDLVATC
                     SVLGLRIVRVLEENSPTALFLHQEQTEYAIRRAISRDYLEPNDELIE"
     gene            14328..15068
                     /locus_tag="VN178_05555"
     CDS             14328..15068
                     /locus_tag="VN178_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HWS80472.1"
                     /translation="MRIEENTFLVAGGGSGLGEATARMLVASGARVVVADLKGEAPEG
                     GRFVETDVTDESSVRAAVNAALEVGGLRGAVNCAGVASAEKVLGREGPHSLDSFTNVV
                     QINLIGTFNVIRFAAEAMVSSGPTESGERGVIVNTASVAAFDGQIGQVAYAASKGGVV
                     ALTLPVARELAGSGIRVMTIAPGIFDTPMMAGLPEAARDSLGEQVPFPSRLGKPKEYA
                     DLVKHIIENEMLNGEVIRLDGSIRMAPK"
     gene            complement(15113..15874)
                     /locus_tag="VN178_05560"
     CDS             complement(15113..15874)
                     /locus_tag="VN178_05560"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HWS80473.1"
                     /translation="MNPLLEQHAYRLSEAGVAGNYTSHGAENNLYKIELLVEHDESNT
                     FGMEDLLWDVGFEEAYDAVAFQIGNPPDREENPGRGCIDPARAAAGLVETGERIEDVA
                     GSGGRLIFATGHPGAMILYYLGLSGWAKELGGDVLTVETRGRYERGVSLDWAESVAAL
                     GDGASLFHTHDPQPMRDVLRQAGPVDLVVADHGFAGAAIAAGVPTIVVMDTNDPAFAV
                     VAGRGADVTVVPMDDNRPLNAYSTALDVVRKVREE"
     gene            complement(16077..16673)
                     /locus_tag="VN178_05565"
     CDS             complement(16077..16673)
                     /locus_tag="VN178_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS80474.1"
                     /translation="MEQSTSEAKRATRRRPGAERLLEAASDLFYREGIRAVGVDTISA
                     KAGVSKRTLYNRFGGKDELVGEYLRRRDEGWRVHLQEQTEGVADPREKLLAVFGAYEE
                     WLVGEDFRGCAFANAVAEIPDPDHPARIVARLHKEGVREHLAALAKEAGFDEPESLAE
                     RLLLLLEGAAATAAIRRSDEPLEVARSVALELLEARSH"
     gene            16935..18299
                     /locus_tag="VN178_05570"
     CDS             16935..18299
                     /locus_tag="VN178_05570"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80475.1"
                     /translation="MLPAFVGVLVADSGRWVAGSGTTVLEDGVLVGDPERPADVGGRG
                     KETIAWLVAPGLALIAVTYGLARFAYGLFLPDMREAFDLSPSLLGLIGAGSYLGYCVA
                     VIISLVYTSRTGPRLMAVGAGAVAVAGMAVVAGSPVAWVLALGILVAGSSTGLASPPM
                     GEAVARSIRPSLQDRANAFINSGTSVGVALSGPAALLLTGQWRVAWTMFALVGLAVLA
                     WNAAVMPRKAVQEEGESGEPDGDHAEAGEVLGRTRLSLSYLIGSRLRSRTLPLFGAAF
                     GVGFASAVYWTFSRDIVVQAGGLGQTGSTLLWTVIGISGLAGGAAGDLVRRFGLTSVL
                     RGGLVSMAASMVLLALASSVLPLAYTSAALFGSTYIMLTGVILIWSVGVFRERPAAGL
                     GAGFLLIAVGQMIGSPVAGALAGATSPAIAFFSFSVAAVLTACVNPHPEDTGASASVS
                     DTQT"
     gene            18491..18913
                     /locus_tag="VN178_05575"
     CDS             18491..18913
                     /locus_tag="VN178_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS80476.1"
                     /translation="MANGLDGKVAIVTGASSSIGAMRRRGGGHVVNISSVAGRQTRPG
                     SGVYSVTKFAVNAISEALRQELIEDKIRVTMVEPRAEETELATHITDEEAQQGVRALH
                     QLDILQAEDIASAVAYCVTRPERVSINEILIRPTEQVY"
     gene            18929..19147
                     /locus_tag="VN178_05580"
     CDS             18929..19147
                     /locus_tag="VN178_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80477.1"
                     /translation="MMLATGALLALLGLVGAAYGIYALVRGGRDQEGGLGPIPERAIH
                     AVAGIRMLIGGAVAIFLGVLAILAALSG"
     gene            complement(19265..20515)
                     /locus_tag="VN178_05585"
     CDS             complement(19265..20515)
                     /locus_tag="VN178_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80478.1"
                     /translation="MDPDASGVEEGKGYLRRGTPVFRRANLALFVAGLATFAMLYCTQ
                     PLLPVFSAEFGVSPAVASLPLSLATGTLAVALILAGSFSEAWGRVPMMTVSLVLSAFL
                     MVLVALSPGFPSLLVLRAVQGVALAGLPAVAMAYLAEEVHPEHAGFAVGLYIAGNSIG
                     GLAGRILSGVLTDAFSWRVALGSLGLACVLASFAFWRILPPSTRFRPRPLIARHLLAS
                     LGGHLTDRDLLRLYAIGALLMGGFVALYNYIGYHLLGAPYELSQAVVGWIFVLYLVGT
                     FSSAWMGRLADSLGRARVLWIGISIMLFGVGMTLASSLGALIAGMAVLTFGFFGAHSI
                     ASGWVGQRAFAARAQASALYLLCYYLGSSIGGSAGGLAYSYAGWPGLVAMVAALLILA
                     LISAARLTLAPRPPEKTPAESLRQ"
     gene            complement(20546..21013)
                     /locus_tag="VN178_05590"
     CDS             complement(20546..21013)
                     /locus_tag="VN178_05590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWS80479.1"
                     /translation="MRHRRRPRRHERLRREAQTGFRGKIVHETKIRVRYAEIDAQGHV
                     NNATYLSYFEVGRVEWLRATGHSYREMERQGRGLVVVKALLHYRRPAFFDDELALITN
                     LAELGKVSLLFDYKVLRDAEVLVTGHTRHACIDLATGKPIRMPEELLELGKGS"
     gene            complement(20938..21711)
                     /locus_tag="VN178_05595"
     CDS             complement(20938..21711)
                     /locus_tag="VN178_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWS80480.1"
                     /translation="MDYVSVKREDDIAVLTVDRQEKLNALDIQVVEEIGQALLDLESE
                     GPRAIIVTGAGERAFIAGADIRAMSIMEPLEAKRFSEIGHAAMALLDRSPVPTIAAVN
                     GYALGGGCEVALACDIRIAAENSTFGFPEVGLGILPGMGGTQRLPRLIGPALAKELIF
                     TGRRISAGEAREMGLVNRVVAEGEALNAARDLAAEISANGPLAVRHAKAAANRTLDVD
                     LVSGLEFEADQFALLFATEDAREGMNAFGEKRKPDFEGR"
     gene            complement(21921..22316)
                     /locus_tag="VN178_05600"
     CDS             complement(21921..22316)
                     /locus_tag="VN178_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWS80481.1"
                     /translation="MARTIRVVVAKVGLDGHDRGAKIIARALRDAGMEVIYTGLHQTP
                     EQVVEAAIQEDVDAIGISILSGAHMTLVPRIIELLAENEAEDILVFVGGTIPKEDITK
                     LKELGVGEVFTPGTPTKKAVEYVKQALPA"
     gene            complement(22361..>23331)
                     /locus_tag="VN178_05605"
     CDS             complement(22361..>23331)
                     /locus_tag="VN178_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80482.1"
                     /translation="LGQYVGDGEDDGGSGYFSIPFVDYARGDGLSVGEGGDRSWDEPR
                     LIADPTPDWVSDYRGLWGLYARDPFEGEDAPAGPMYNRDKSVSRAWYDPVGWAGLDKV
                     APPTETLEAILEQRADIISRCSTLRTEIEDKSRQLKKLGAELAAMRDRSHLDAPYRAG
                     TRRVAELSRELDRLRGKLATDEAISESLAEYADRLRAGERDQARAHISRAHKPASEDE
                     LRVSRVAEAWAAISVSLMLISFVGIAMFEREHIISMLVFSIAFFAFVEAGFRGRLVNL
                     VSSANIGLAVVATLIIFYEFFWQLVVAAVLVIGLYVLWDNLRELRR"
BASE COUNT         4244 a         7557 c         7385 g         4145 t
ORIGIN      
        1 tacgacgagt atgccgaggc ccgccgcgac gatctcagga aagaggccca ggagatactc
       61 gacgaacagg tccggaagat agaagaggcc ggtggcaccg tggcggtcgc ccacgtgaag
      121 atggatgaac agagggatga ggcgatcgtg catcttgccg aggatatcga cgccggcatg
      181 atagtgatcg gcagccgcgg gttcggtggg atgagacgcg ccctcatggg taacgtcgcc
      241 gactcggtgg tccgccacgc ccactgcccc gtcctggtgg tgcgcccgac agggggtatc
      301 agccggccgt tctagcacgc tgcccggctt tgccaggcgg cttgtcagca cggcggctgc
      361 gccgcctctt cagtatttca tccgatgtgc aagtttggtc ggtgagcagc tcccagaacc
      421 tcgtcggggg tatcccttcc ctccggcaaa acagaaccgc catcgtgtgg ctcagggtgg
      481 cctgggccga gccaccccta gtatcgagcc ggccctcttg ctctctcctt gaggctaccc
      541 tgtggcggtg gtgggcttcg gaccccggtc gggtgaaggg ttgtacttga cccactcgca
      601 gtcgggggcg accacatttt tgcggtcagc gcccacccta tgcggtgtcc atagctcagc
      661 cacttatgcc gcctaccagc ctgtagcgtg gcgcagatcg tacagatccc cgtgccacgc
      721 atccgcgtcc attagcttgc ctgctatctt ggccgtgctt gtaaactggc tgaccttctt
      781 gctgcggttg ccccagcggg ctttggcaaa cgggtgatag ccggcgttga ctatctcgtg
      841 ccagagtacg gaggcgaggc cgcgcttgga gccaaggtag taggcggcat cccagaggca
      901 cacgttgtca aacgtgctta ggtctatgcc gcctaaggag tcggtgtagc tcttgccagc
      961 gtacacaaat atgagcggct cgcggcctat gcgctcccgc agctgctgta tgaacccgta
     1021 cagatgctcg ggcttgggac taagactcgg gaagtcaggg tacgcctcga aatctatcaa
     1081 tgggcactgg ccttgacccc aaccgccagc accggccaca tgcaggaagt gcgcaacctg
     1141 ctcgcggatg ggcgcgttta cgaggaagtg gtaggggaag cgcacaaagc ctgcctcctt
     1201 ggagcgccga gcgtttatgg cgtagtaggg gtcggtgtag cgcttgcggt tagggtatgg
     1261 tccctccgtt gcctttatgc cgcagaagcg aaattcctct tgccatatcc tgtgccagtc
     1321 aagctcgccc tgccagtggg atacgtcgat gccatacacc acatcgagca ggtcgcgctg
     1381 tgtggcctca tctaccgtgc gctcggcatg ttgctgggcc cggaacccct cgggcgtaag
     1441 gatgcccgct ttcatcccgc caaactcgtg ctctacctgc gtatcactct tgatacccgc
     1501 gtaaaagcgc tccaggtcag ccgcctgtcg tgcagcttcg gcttggcgct gtttgagtag
     1561 cgtccccgcc gccacggcgg ccacggcagc cgccttgagt acctgccgtc gagagatacg
     1621 gccggtggcg agggcacgcg tcagatcgtc gaatcgctcc tgattcatct cccactcctc
     1681 ccgcacaccg tgccctaaag aaggctagat gtgtgaagcg ctactcgcgt gtggaagatg
     1741 tcgtatctgt tgggtcgcat ccgctcctta tcccccctat aagggcggag cattgttacc
     1801 ccgccgctat tgctgttcta cattccggct accgcccata gaatgaaggt tcatgggctt
     1861 tacaccccgc ctcacagcgg gtttgccttt ttcgcggcgc cggagcgcca gcgcttgtgg
     1921 actatggtac taacttggag gcctccagtc tataggtcag atggccattt atctgaagag
     1981 aggctggcta tatcttattt gctaggaggc tatttctgag gatgaacttc gtagaacccg
     2041 taagggcgaa gttcgcagca tacatcggcc aggaacaggc actttcttcg ctctggctct
     2101 ggtttgggcg ccagccttgg cgtagtggag gtctcgagct tcgttcgagc cctcagagat
     2161 acttgcacat cgggtatttc agcttcttca tcgttctggt ggcgtggttc ggagctggtg
     2221 tcttggggtg aagaaggaac ggtacgagag cgtcccgacc aggatcgcgg cgatggagac
     2281 gctccccaag agcatgtaga gtacgactat ctgcagccgc accgcttcga gcgggtcggc
     2341 gccagcgatg atcatgccta ccatagcacc cggcaggaag attatccccg ttgtctttgt
     2401 cgcgtctatg agggggatga gggcacgttg gagcgcggtc ctcaggatgg gcgctatggc
     2461 ctggcggctc gtagctccga gggcgagggc cgcctcgacc ttgggcatct gttcggccac
     2521 gtcgtcgcga atgcgcgtca tcatcaggct cgcggcgttc atggagttcc cgatcaccat
     2581 gccgccgagg ggaatgaggt accgggccgt ggggggtacg atccggagcg tgagcagcaa
     2641 ccccaacgtc gccacggccg ctgccccaac ggcgacagca gcgatgagaa aggcccgcgg
     2701 cacatcctca gccctgctcg ccgaggtcca ggcggcgaag aggaccatac cggccaacag
     2761 cagcaccacc gccccgatac tcttcagccc aaagatgaag tttatgacgt accctatcgc
     2821 ggcgagctgc aagaaggagc ggaccacggc gacgccgata tctttctcca gatccaacct
     2881 ctcgtagagg ctgaggacta tcgcaatggc cacgagcccg agtgtcagaa gcgccgagcc
     2941 cagcgtctgc agatccgcca tcctagagcc tccccgcaat gaactgccgc gcccgctccc
     3001 ccgcgccaga gaagaaatcg cccctatccc acactcctac gctctttcct tccgccagta
     3061 cgaggacccg gtcggcgacg cgctcgacct gggcgaggtc gtggctgacg aagaccatcg
     3121 tgagtccgag cctggcgttc agatctctga cgaggtcctc gatcctggtc cgcgcggcct
     3181 cgtcgagggc gctcgtgggt tcgtctagca ggagcgcctc tggcgccagg gcgagggcgc
     3241 gagctatggt ggcacgctgc tgctgtccga cggagaggga ctgcggatca cgccccgcca
     3301 acgaggcgtc cagccctacc atcccgagca accgtcgcgg gtcggcgtcc ctgcctaaga
     3361 ggcgcgcccc gtagagaacc gcctcctcga ccgagtcccc gaatagcgct ggaacctgga
     3421 agaccattcc gaccctccgc cgcagctccg acgggtcaag ggtgctcgtc ggttccccgc
     3481 tcaggtagat ctcaccagaa gacggctcta tgaggcggtt gatggcgcgt aacaaggtgc
     3541 tcttcccagc ccccgatggc cccaccacgg cggtgatctc accgtcaggc atccccgcgt
     3601 cgacgccttt cagaatctcg acgccgccga ccacgtaccg gagcccgcgg acctctatct
     3661 tgggccggtc caagctagag ggtctcacgc ctcaggtagg cgtagaggcc atcgtagagt
     3721 gtgtcggtgt gttccgcgag cgtatggtcg tcgggcagag cgagttgcat gccgcgcacg
     3781 atggcgtcca acccctcgga ctcgggcctc ccgtacttct cgtccacgag gtcggcgtcg
     3841 tggacgatct cggctatctc gtggagcacc gggtcctcga gaccgtactc cttcaggaaa
     3901 gtctcgaagg agcagtcctc ccctcggtgg gatagcctgg ccccgaccac gtcgaagagc
     3961 tccgccccct ccggcgcctc cgcgggatcg ccgaagaagg agaagtcggc ctccgggtcc
     4021 aagaagcgcc ttatcagcca gatgcaggcg gtgcggtcca cgtggcagcc ccggcgcgtg
     4081 gcccaaagca tcagcgctta ccttcgccgc cgagggtccc ggccgactct tggccggccc
     4141 tcgctgcttc gcgggtggcc ttgagtgcct ctgcagcctc gcctctgagc ggggaacgga
     4201 agaagtcgcg gcgcctctcg gcccggaaac ggcgctccag cttgccgagc tgttccaaca
     4261 cgccaccgcc gcccatccgg gccttgcgcc gcaggcgacc tgcttcggcg atgatggccc
     4321 ggtaggcctc ctcccgtgag tgacggaagt cctccaccag tccggcctcc tcggccaccg
     4381 tgcccgtccg ggcttcccac agggtagcat cgccccccgc ctccctcacc ctgagctgca
     4441 gccactccag ctgctctcgg gtgacggcat cctcgggcag gaccgccacc ccgtcctgga
     4501 ggtagagggc acccaggtcc ctgagcttgc gccacacggc cacgcggtgg cgcgagggct
     4561 ccctcgggag gcggtagacg agcactaccc agcgcgttct ctccaaggac gtcagcctac
     4621 cccgtcgacc gatacggacc tacgcgggcc acgcacgggc acgccttaga gccccctccc
     4681 aggcttccag cccgccctcc aaggagaacg cttcgtaacc ccgttgccgc agctgctccg
     4741 cggcgaccgc gccggcgcgc ccgcggttgc agaaggtgac gacccgagtg ccctcaggca
     4801 gctcgaactc ctccatggcc tgcccgtctc cggccttcag ggcgtcgtag gcgtcgaagt
     4861 tcacgctccc gggtatcgac ccctgcgcat actcttcccg cttgcggaca tccaccaacg
     4921 cgatccgctc cccgcgctgc agcatacccc tcaaggtcct cgcgttcacg ttccgttgcg
     4981 tcactatcgc gctcccccgt tctctggtca ctcctacagc agggtccata ccatccccag
     5041 caccgccccc gcgggcacca tcaggtatat tggcaccttg tagcgctgga gcaccacgaa
     5101 cgagacgacc gccaccacca acgcgaagac gtcaagacca tccagggaga aaccgtcggg
     5161 gaacaggacc tgcgtggcga agtagacggc caggttggcg atcacgccga ccacggccgc
     5221 cgtgaccccg atgaggatgg ccttcaggcg gcgatcgttg gccaagagct ctatgtaggg
     5281 agccaggaag aagatgaaca aaaagcacgg caggaacgtc gcgtaggtgg tgatcaaagc
     5341 cccgagcgta ccgtatagca ggggcgagaa ggggccggga tcgttccagg cgccgataaa
     5401 cccgacgtac tgggtgacca tgatcaaagg ccccggcgtg gactcggcca gtcccagccc
     5461 ttgcaccatc tggtcggccg tcagccaccc gtagtggttg accgccacgt ccgagacgta
     5521 gctgaggacc gagtaggccc cgccgaacgt caggaaggcc gcccccgtga agaacagcgc
     5581 ctccttgatg agcacgtcat ccccgcccct ccacaggtag agcgccccca acggaagagc
     5641 ccacagcagg acgaaggtcc ccagaagcct ggcgttgcga agcaaagatg gcccgccgtt
     5701 cgggtcggtc ctgtcgatgg cggactcttc ttcctccgac gagtcctgcc ccccacccgc
     5761 aaaggcgtcc ccggcgaagc ccgtgcggct caagaggagc ccggccacgg cggcggccag
     5821 gacgaccagg gggaagggca cggaaaggaa gtagagggcg acgaaggcca ctatggcgaa
     5881 gcccactggc atcctgtggt tgatggtgcg cttgccgatc ctgaggacgg cctcgaccac
     5941 gatggctatc accacaggct gtatgccgta cagcagcccc gtgatcgccg gcacgtccga
     6001 gtgggcgacg gccaggtacg agagcaagag cagaacgaag atggatggga tgacaaagaa
     6061 ggaaccggcc accacccctc ccaacgtccc gtggagtctc cacccgatgt aggtcgctac
     6121 ctgctgcgcc tcggggccgg gcaagagcat acagaagttg agcgtcctca ggtactgctt
     6181 ctcagagacc cagcgcttcc tctcgaccag ctcgcggtgc atgatcgcga tctggcccgc
     6241 tggccccccg aagttgatga acccgagctt cacccaaaac cagaaagcct cccagaaccc
     6301 gaccggcttg gttactgttc cccctcttgc agccttctcg ttctccaagt tatctctact
     6361 ttcttagtga acaatctaga gtacagtaac catggttaca tcgaaagtca agacggagac
     6421 cgacgattct agcgtttttt gccgtagagg cgctctctgt tgatggtgta gaagtccatg
     6481 gactcgtaga ggcttctggc ggcctcgttg tcgtggatcg cgctgacaaa agcagtttgg
     6541 gcgccgatcg ctcgcagcct gcgtaggcct tcttgcatta gggccttacc cagtcctttc
     6601 cctctgtgcg tttgcctggt accgaggggc tcgaagaggc cggtgaggct cttcgggtcg
     6661 aaccagcaga tgccgtagga gccgaactca ccgtcggggg cgacgacgac gaggtcgagg
     6721 tatgggttgt agcccggcgc ggccctcagc cggcggtatg cttcgagggt gacccgggag
     6781 ggatgccaga cctcgcggtg gacctccacg cgcgctctcc actccccctc cccaccgacc
     6841 tcgcgcactg tccacccatc gggcagggcg tgcccgggaa gcggctcgtc gagcgtcctg
     6901 accatcttca gcgcatggtc gggatcccgc ccgaagccca ggccggcaag gagcgcatcc
     6961 agccgcgggt cgtcttcggc aacccgcgtc cacaactcgt ccccagctcg ctccccgtaa
     7021 accgcgcgag tctggcccgc cgcccactcc agcatctcca tctccagcga gcccgagcca
     7081 cgaagacgag gatgcacctg catcatcacc ccatccggct cctccaaaag ggcaaagccg
     7141 accaactcgc cgccctcttc ccagaatcgc acctcctgac gggcatcgaa caccgtattc
     7201 tggtacatcc cccagatcac gtcgccgggg tgccagtatg cccctagcgg ggcgacggac
     7261 gttgccgaag acaagaactc cagcatcgcc tggaggtccg cctcgccacc ataagcccgg
     7321 gacctcacag cgggtcccgg tagataccgg aggcaccaca gagttcgcca ctgcccacga
     7381 gccccgtcgc agcagtggat tcccgctttc gcgggaatga cgcaagtaga aggtgtgaaa
     7441 cgcccgcgac tcgaacacca tcccgcccga atgagtccgg catcagcccg tcccccctcc
     7501 cccgcaaagc gctaacgaag aagcgaatcc tccaccggct cacgcccacc cagcacaaaa
     7561 ctgacaagcc tgcgaagcag gcgtgctgac aagcgaccga agggagcgtg ctgacaaggc
     7621 gcgaagcgcc gtgctgagat gctaatgatg cacccaggct tcgggatcgc tggtgagctt
     7681 cgtgatcgag gaggggagct ttccgtcgac gatatcggcg agggttacgg actcgaggac
     7741 ggcgcgcagg ttggcgcgga cggcgatcca gacctcctgc aggtgctcgg ccgcgccctg
     7801 gtactcgacg gcctcgggcc actcgcccct gacgttcgcg aggggtcctt cgacggcgcg
     7861 gatgatggcg gcgagcgaga tctcctcggc cggcagcgcg agccagtagc cgcctgaggc
     7921 gccgcgcctg gtacggacta tccccgagcg tttgaggtcg tggagtatgt tctccatgaa
     7981 cttcttgggg atgccctggg tctccgagat ccaatccccc tttatggggc cggcgtcctc
     8041 ggtcgcagca agggccagct cggaggccgc ccttagagcg tagtcggtct tggccgagac
     8101 ctgcaagcta cctcacgctc cccggaaggc cgtatccacc ggtctcaacg ttcatcgtcg
     8161 ccctttctct cgccctgctc aggcacgtag acgaaccagt aagaccctat gagtatcccc
     8221 aggaagaaca ccagcgcccc ggcgaactgc aggtactccg tcgccatcca acctcctctt
     8281 cgttccggaa acgtcagacg ccccgccaca caattctacg atagcagcgc cggcttcgcc
     8341 gccaggcact ggttcgaggt catggtaact tccgaggagg gctcgtcatc gttccctggc
     8401 cggcctttgc gctggccgcg ccaggtactg gacgtagccg cgcgggttgc gttccgcctc
     8461 ttcgcccagc tcgctggccc gccactcggc ctgctccagc gtcgcgtagc gggccacaac
     8521 tatcttcccg aagagcgtca ccgccaccac ctcgaagcgt tcctcttcca cccggctcca
     8581 gtcttgaccc cctttcattc tgcctgctaa gatcgccttt agcaacgctt tccgggagag
     8641 ggagtacagg cggccgtatg cgcgacgggc catgcacccg acttatagag gagacgagat
     8701 gctagaagga cttgtccagg acttccaact tacccttccc tattttctgc gccgggccga
     8761 acagctctac gggcaccggg agatcgtcac ccgccttccc gacgagtctt tccacagaca
     8821 cacctacgcc gacttcgtct ctcgctccaa gaagctcgcc gtggccctgg ggaacctcgg
     8881 cctggagccg ggggataggg taggcacgct cgggtggaac acctaccagc acctggaggc
     8941 gtacctcggc gtcccttcgt cgggactcgt gttgcacacc ctcaatccca ggctgcacga
     9001 ggacgacctg gcctacatcg cgggccacgc cgacgacagg gtcatgctcg tcgatgagac
     9061 ccttgtgccc gtcttcgaga agttcagggc gcgcacgaac gtcgagcatg cagtggtcat
     9121 gacggagggc ggggatgtgc cggacggtat ggtctcctac gaggacctga tcggggactc
     9181 cgacgaggcc gacttcgagt accccgactt cgacgagaac caggcggcgg cgatgtgcta
     9241 cacctcgggg acgacgggac gtcccaaggg ggccctctac tcccaccgct cgacggtgct
     9301 ccactccatg atggcggcga tgggcaatac gttcgcgctc tccgaggccg actgtgtgtt
     9361 gcccgtggta cccatgttcc acgtcaacgc ctggggcctg cccttcgcct ccgtcctcgt
     9421 cggggccaag ctcatgatgc ctggcccgca tctggacgcc gcaagtctcc tcgaagacct
     9481 ggagcaagaa gaggtgacgc tcacggccgg tgtcccaacc gtcttcctcg ccgttctgca
     9541 ggctctcgat gcggagcccg agaggtacga cctgtcgaag ctgcgggcga tggtcatcgg
     9601 cgggtctgcg gcgcccaagg gcgtcataaa agactacaag cggcacggga tagaggtcgt
     9661 ccacgcctgg ggcatgacgg agatgagccc catcgggacc gtggcaaacc tgacgaccga
     9721 gatgaagaag ctccccgaag aggagcagct ggagtacgag gccaagcagg gctaccccgt
     9781 gcctttcgtg gagatacgcg cccgcggcga cgagggcctg atcccgtggg acggggagtc
     9841 aatgggcgag ctggaagtcc gcggtccctg ggtcgcgagt tcttactacg aggcgccaga
     9901 gggcgacgac aagtttacgg acgacggttg gttcaggacg ggggacgtcg ttaccataga
     9961 ccgtttcggg tttatcaaga tcaccgaccg cagcaaagac ctcataaaga gcggggggga
    10021 gtggatctcc tcggtcgagc tggagaacgc gcttatggcc caccctgccg tctcccaggc
    10081 ctgcgtaatc gccataccgc acgagaagtg ggatgagagg cccctggccg ccatcgtcct
    10141 cagggagggc gagagcgcga cggaggacga gttgcacacc cacctggaag tggacttcgc
    10201 cagcttctgg ttgcccgacg cctacgagtt cgtggaggcc atcccgatga ccgcgacggg
    10261 caagttccag aagctgaagc tgcgcgagca attcgaaggc tacacgtccc ctaagagcta
    10321 gcgtgaaggt cataggggcc ggcttcggcc ggaccggcac gatgtcgctg aaggtcgcgc
    10381 tcgagaccct gggcttcgac ccttgctacc acatgaccga ggtcttcgcc cacccggagc
    10441 atgcggggtt ctggatctcc gcctggcggg gagagccggc agattgggat agggtcctcg
    10501 gagaatatga ggcggccgtg gactggccgg cctgcacttt ctacgaggaa ctcatggagc
    10561 gcgatccagg cgcgaaggtg atcctgagcg tccgtgaccc cgagcgctgg tacgagagcg
    10621 tgaggaacac catctacgag ttgagcgtgg tcatcccccg ccaccccatc taccgcatcg
    10681 gccacaagct cgtcagcttc ttcgtgtttc gtgggccagg aatcgtcgac ctggcgagcg
    10741 agatcatctg gcggggaacc ttcgacggcc gcttcgagga taagacctat gccacagggg
    10801 tcttcgagcg ccacagcgcg gaggtccagc ggcgtgttcc cgaaaacagg ctgctcgtct
    10861 acgatgtaaa ggcaggttgg gaaccccttt gcgagttcct cggggtcgaa gtaccagacg
    10921 aaccattccc ccgcacgaac gacacggcac agatgcggcg caggcagcgg ggagtgaagg
    10981 cgctctcctt tgtcgcgccc gtagcgctcc tcgtcttgct gtttgcattg ctcgtcctga
    11041 ttgttagggg caccgggcgc tccccgctca actccctgcc tgcgcggagg ctgcgaagct
    11101 aggcgcggga cgttcgacga tgggccagtg cagaccggcg gccacgatgg cgaggacgat
    11161 gccggcccac cagatcggca tgaaagagcc ggtctgctcg aaggcgagtc cgccgagcca
    11221 caccccgagg aacgcaccga cctggtggct gaggaagacg atcccgaaga gcgtgccaag
    11281 gtacctcgtc ccgaacatca cggcgacgag ccccgaagtc agcggcaccg tcgagagcca
    11341 cagaaccccg atcgcggccg caaaggcgag caccaccgcc ggggtcggag gcagtgaggt
    11401 aaaaagcgct atgacgacgg ccctggcaag gtagatcccg gcgagcaggc gccgcttgct
    11461 gtggcgtgcg ccgaggactc ccgagccgta tgaccccacg atgttgaaca gccctataag
    11521 cgccagggcc cacgccgcca cagcaggccc cgcggtgtgc atgcccccgt gcgagtgccc
    11581 agcgacggag gtgatgtgcc ccggcaggtg cgtggtaatg aacgccacgt ggaagccgca
    11641 cacgaagaat ccagcaacga gcagcaggta gctgccgtgc ccaaaagcct gccgaaccgc
    11701 gtcacgggcg ggcaaagcag gctccttcat accctgccgg cctaccccgc cgcccttgag
    11761 ggcggcggcg agcgcgggaa cgaccaccat gcaaccggcg agcagcagga gcgcggcctg
    11821 ccaaccgtag tccgcgagga acgcctgacc gagcggggcg aacacgaact gcccgagcga
    11881 acctgccgcg gtcgcgagac ccatcgcctg cgagctcctg tcagggggaa caagcctccc
    11941 caacgcggcg atgacgatgg tgaacgaagc accggagagc ccgaggccga cgagtacgcc
    12001 ggccgtgagg ttgaaggcga gcggcgtcgg gcttagtgcc atgagtgcga cgcccgcggc
    12061 gtagacaaca ccaccggcgg cgaggatgcg ggccgaaccg taccggtcgg cgacggcccc
    12121 cgcgaacggt tgtcccaggc cccagacgat attctgtatc gctatggcga gggcgaatac
    12181 ctccggcgac caaccgcgcc cctcgctaat cggcgtggca aacaggccga agctcgtcct
    12241 caaaccgtag gtgatgagcg cgatcaaaca cccgcacccc acgacgaacg ctgtagactt
    12301 ccaccatcgc gacttcccgg ctaccaaact catgcctcct caatccgatc caacgctcct
    12361 cactatggac tcggcggaac gatcggtcaa tcacagggga gttgcgccat atctctgaga
    12421 ccttgatccg gggctcgggt agactccagg ggagtctatg tccgactatg gaagggaagg
    12481 ccgttctgga cgaaacacca gggacatcga gccgggtggg atctacgagt aagtcataga
    12541 cctgtgggct aagggcatca ccttcctccc ggggcatagg atgcgggtgg agataacatc
    12601 cagctcccat ccccgctggg agcgcaacct caacacgggc gagagcacac tggagtcctc
    12661 ccaaaccgaa gtggcccacc agatcatctt ccacgacgct gggcggtgga gcagggcaac
    12721 cctaaccgta gtagaaaggt agagggatga acgtctcgaa agcggttctg atcaccggct
    12781 gttcctcggg catcggatgg gccactgccg agcgcctggt ggacgtgggc tggagggtct
    12841 acgccacggc ccgcaatgtc gagaagatgg ctccgctcga acggcgtggt tgttaccttc
    12901 tacccctcga cgtgaccgat gaggactcca tgcgctcggc ggtcgacgag atcgaaagtc
    12961 gcgaggggcc gtcggggtgc tcgtcaacaa cgccgggtac agctagtcag gggccgtgga
    13021 ggctgtgccc atggagaagg tcaggcggca gttcgagacg aacgtcttcg ggctggcgcg
    13081 gatgtgccag ctcgtgttgc cggggatgcg gcggcagggg tacggcagga tcgtcaacgt
    13141 ctcctccatg ggcggaaggc tgacgttccc cggcgccggc tactaccacg cgacgaagca
    13201 cgccgtcgag gcgctgagcg acgccctgcg cttcgaagtc cagggcttcg gcgttgacgt
    13261 ctccgtcata gagccgggac tcatcatgac cggcttcgca gagaccgccg tcggctcgat
    13321 ggacggctca gacggcgagg acccttacac tggcttcgat gaagccgtcg ccagagtaac
    13381 ggcggagaac tacgagcggg gtctcatctc caggctgggc ggcggaccag aggctgtcgc
    13441 cgaggctata gagcatgcgc tctccgcccg cagccccagg agccgctacg ccgtcacgcc
    13501 ttcggcgcac ctgtttatgc ggctcaggcg tctcctgccc gaccgtgttt gggatgccct
    13561 cttgagagca ggcccttatt accaaccagg ccgaaagccc cggagatgat cgggcttccg
    13621 cgcctgtcgc cgctaacgcg aacctgggcc atagcccagt tcttgggttg gtgtacccgc
    13681 cggggctttc tcttcatgca cgtaccctac cgtatatgag ctgagtacgt atagaactag
    13741 ataggtatat ggttagtttc tactcaatac ttatcagaat cagatctgaa gcctacaatg
    13801 aagggtgacc gagacaccgc accagggaga tgcgcatgga acaatcgatg gacgcgcttt
    13861 cgcaggtcat aaagagcgag cgcgccatac gtggctggtc gcaaggcgag cttgccgaga
    13921 gatccggcgt gagccgcaag acgatctccg acatcgagaa cggttctctg aagcgcgcac
    13981 cgcagctacg caccgttcac aagctgctct gggtattgcc aacggaagcg ccgccgtccg
    14041 gcgcgaagcc gccccaagaa gcgtgggaag agatcaaggg attcgtggac ctgctgaaca
    14101 agacgttcga aggcggcacg actgatctgg tagcgacttg cagcgtcctg ggcttgcgca
    14161 tcgttcgcgt gctggaagag aactcaccca ccgcactgtt tctccatcag gagcagacag
    14221 agtacgcaat ccgcagggca atctctcgcg actatctcga gccgaatgac gaactcattg
    14281 agtaggagca ctatatctgc aagcgatctc aaccggggag gtagaaagtg cgcatagagg
    14341 agaacacgtt tctcgtagcc ggcggtggct ccggtctggg agaagccaca gcccgaatgc
    14401 tcgtcgcctc gggagcccgc gtcgtggtcg ccgatctcaa gggcgaggct cctgaaggcg
    14461 gacgctttgt cgagaccgac gtcactgacg agagcagcgt gcgggcggcc gtgaacgctg
    14521 ctctggaggt gggagggcta cgcggcgccg taaactgcgc cggggtcgcc agcgcggaga
    14581 aggtcctcgg aagagaaggg ccgcactctc tggactcgtt cacgaatgtc gtgcagatca
    14641 acctcatcgg caccttcaac gtcatcaggt tcgcggccga agccatggtc agcagtggac
    14701 cgaccgagag cggagagcgc ggcgtcatcg tcaacacggc cagcgtggcc gctttcgacg
    14761 gccagattgg gcaggtggcg tacgccgcat ccaaaggcgg cgtcgtcgct ctgaccctcc
    14821 ccgtcgcgcg cgagctcgcc ggcagtggca tccgcgtcat gaccatcgcc cccggcatct
    14881 tcgacacccc gatgatggcc ggtctgccgg aagcagcccg cgactcgctg ggcgaacagg
    14941 tccctttccc ttctcgcctc gggaaaccca aggagtacgc cgatctcgtc aagcacatca
    15001 ttgagaacga gatgctcaac ggcgaggtca tcagactcga cgggtcgatc aggatggcgc
    15061 ctaagtagtc gctcgggaga gtgggggcag gagattggtt ctcgctcgtt ccctactctt
    15121 cgcggacctt tcgaaccaca tcgagggccg tcgagtaggc gttcagcgga cggttgtcgt
    15181 ccatgggtac gaccgttacg tcggcgcccc gcccggcgac cacggcgaag gccggatcgt
    15241 tggtgtccat caccacgatg gtggggacgc cggcggcgat cgccgccccc gcgaaaccgt
    15301 ggtcggcgac caccaggtcg accggtcctg cctgccgcag aacatccctc ataggttgcg
    15361 ggtcatgggt gtggaacagg ctggccccgt cgccgagagc ggcgaccgac tccgcccagt
    15421 cgagggatac tccccgctcg tagcgtcctc gcgtctcgac ggttagaacg tctccgccta
    15481 gctccttcgc ccacccggaa agcccgaggt agtagaggat cattgctcca ggatgcccgg
    15541 tggcgaagat caagcgtccc cccgagccgg cgacgtcctc tatccgctct cccgtttcga
    15601 cgagcccagc ggccgcgcgt gccggatcta tacacccccg gcccggattc tcctccctgt
    15661 ctggcgggtt accgatctgg aaggcaacgg catcataggc ctcctcgaac cccacgtccc
    15721 acagcaagtc ctccatcccg aaagtgtttg actcgtcgtg ctcgacgagc agctctatct
    15781 tgtagaggtt gttctcggca ccgtgcgagg tatagttgcc ggctacaccg gcttcggaga
    15841 gccggtaagc gtgctgttcg agaagcgggt tcacggagcc tgagtttagc agagcaagcc
    15901 caccgcaagt gagcgccgtg cttgctctgc tgaccctagc agcaactgct tccgggacga
    15961 gatgccgacc tgcctcgccc tctattttcg tttcgaccaa ggttggtcga gatgtatgcc
    16021 gtttgtgggc gcagattgag aaccgttcgg tcgtaaggga tcgggaagaa ggaactctag
    16081 tgcgaccggg cctccaggag ctccagcgcg acggaacgcg ccacttcgag gggttcgtcg
    16141 ctgcggcgta tcgccgccgt cgcggctgcg ccttccagca acaatagtaa ccgctccgcc
    16201 agggactcgg gctcatcgaa tccagcctct ttcgccagag cggccagatg ttctctaact
    16261 ccctccttat gcagccgcgc tacgatgcga gccgggtggt ccgggtcggg tatctccgcc
    16321 acggcgttgg cgaacgcaca gccccgaaaa tcttctccca ccagccattc ctcgtaagct
    16381 ccgaagaccg ccaggagctt ctccctggga tccgccaccc cttcggtctg ctcttgcaga
    16441 tgcactctcc acccctcatc ccggcgacgg aggtactcgc ccacgagttc gtctttgccc
    16501 ccgaagcggt tgtagagggt cctcttggag actcccgcct tcgccgagat ggtatccacg
    16561 ccaacagccc ttatgccttc ccgataaaag agatccgaag ccgcctccag aagccgctcc
    16621 gccccaggcc tcctcctggt agcacgcttc gcttcgctcg tactctgttc catcgtccaa
    16681 tcctcgacct ttctctcgtc tgaccccttg attatacagg tctgtgtact atactccatt
    16741 cataaactac acaggtctgt gtagttttgt atagtggacg cggtagcgtc ggcagtatga
    16801 ggaggtgtcc gagttgtctg ggagattggt cgggattacg aagtggttcc ttgggtttgt
    16861 gcgtgaggtg gatcgtggca tcgccgtagg acgcgggtac gcgccggtcg gaaagaagac
    16921 taaggggaaa cggcgtgctt cccgcgttcg tcggggtgct ggttgcggac agcggacgtt
    16981 gggtggccgg atccggaacc accgtgttgg aagacggggt gctggtgggt gatcctgagc
    17041 gccctgcgga cgtgggggga cggggcaagg agacgatcgc ctggctggtg gcgccggggc
    17101 tggccttgat cgcggtcacc tacgggctcg cccgtttcgc ttacggtctc tttctgccgg
    17161 acatgcgcga ggcgttcgac ctttccccct ctttgctggg gctgataggc gctggttcct
    17221 acctcggtta ctgcgtcgcg gtgatcatct cgctggtcta cacgtcgagg acggggcctc
    17281 gcctaatggc ggttggtgcg ggcgcggtgg cggtcgcggg catggctgtg gtggctggct
    17341 cgccggtggc gtgggtattg gctctgggga tactggttgc cggcagcagt actgggctcg
    17401 cctccccgcc gatgggggag gccgtcgcgc gttcgatccg gcccagtctt caggacaggg
    17461 ccaacgcctt catcaacagc ggcaccagcg tgggggtcgc actctctgga ccggcggcgc
    17521 tgctcttgac cgggcagtgg agggtggcgt ggacgatgtt cgcgctcgtg ggactcgccg
    17581 tcctcgcgtg gaacgccgca gtgatgccgc gcaaggccgt gcaggaggaa ggcgaaagcg
    17641 gcgaaccgga cggagatcat gccgaggcag gcgaagtttt ggggagaacg cgcctgtccc
    17701 tctcttacct gatcggttct cgcttgcgct cgcgtacttt gccgctcttc ggcgccgcct
    17761 tcggcgtggg cttcgccagc gccgtttatt ggactttttc gcgcgatatc gtggtgcaag
    17821 cgggtggtct gggccagacg ggatccaccc ttctctggac ggttatcggg atctccgggc
    17881 tagccggtgg cgcagcgggc gatctggtgc ggcgcttcgg gctaacgtcc gtcctgcgcg
    17941 gcgggctcgt ttcgatggcc gcctcgatgg tgttgctcgc gctcgcctcg agcgtgctcc
    18001 cattggctta caccagcgca gcactcttcg gttcgaccta catcatgctc accggcgtga
    18061 tcctgatatg gtcggtcggt gtctttcgag agagaccggc cgccgggctc ggagccgggt
    18121 ttctcctgat cgccgtagga cagatgatcg gctctcccgt agccggagcc ttggcaggag
    18181 cgactagccc ggcgatcgcc ttcttctcgt tttctgttgc ggcagtgctc accgcttgcg
    18241 tgaacccgca tcccgaagac accggagcat ctgcttccgt ctccgacacg cagacatagt
    18301 ctatcccgac aaggattttg cccactccag acagcgcacc cgtctcggtg agcttcgcct
    18361 acatatttcg ggccattctc gtcctgcacg gcggggcggt ccgttttttc ggcctttatt
    18421 cgggtaaacg tatgactcac gtactggtaa ggatgagagt atcacccgac atcaggaagg
    18481 atcagataac gtggcgaatg ggctagacgg caaggtggca atagtaacgg gcgcttctag
    18541 cagcataggc gcgatgcgcc gccgcggtgg cgggcatgtc gtcaacatct ccagcgtcgc
    18601 cggccgccag acacgcccgg ggagcggcgt ctactcggtg acgaagttcg cagtcaacgc
    18661 tatctccgag gccctgcggc aggagttgat cgaggacaag atccgggtga cgatggtgga
    18721 gcctagggcc gaagagacgg agcttgccac ccatatcacc gacgaggaag cccagcaggg
    18781 ggttcgggcc cttcatcaac tcgacatcct ccaggccgag gacatagcct ccgccgtggc
    18841 ctactgcgtc acccggcccg aacgggtgag catcaacgag atcctgatcc ggccgaccga
    18901 gcaggtctac tagcggcggg agaccgccat gatgctggcg acgggagccc tcctcgccct
    18961 cttggggttg gtcggcgcgg cctacggcat ctacgcgctc gtgcgaggcg gacgggacca
    19021 ggaaggagga ctggggccta tccccgagcg cgccatccac gcggtggcgg gcatcagaat
    19081 gctgatcggc ggggcggtgg ccatcttcct gggcgtcctg gcgatactgg ccgccctctc
    19141 cggctgaacc cgagcagcag agggctcgtg ggatcgtcgg ctcagctctt cttacggttc
    19201 cttgcaaccc cacctaacca gacgaccgtc atgtcaccct cgggcagact cagaagctac
    19261 tgacttattg ccgcaagctt tcagccggcg ttttttccgg aggccttggg gcgagcgtta
    19321 gcctagcggc ggagatcagg gcgaggatca ggagggcggc gaccatggcg acgagacctg
    19381 gccagcccgc atatgagtag gcgaggccgc cggcggagcc gccgatgctg gagccgaggt
    19441 agtagcagag caggtagagc gcggaggcct gggccctggc ggcgaacgcc ctctgcccca
    19501 cccagccgct ggcgatggaa tgggcgccga agaagccgaa cgtcaggacc gccattccgg
    19561 cgatgagcgc cccaaggcta ctggccagcg tcatgccgac gccgaacagc atgatcgaga
    19621 tcccgatcca cagcaccctg gcgcgaccca ggctgtccgc gagacggccc atccaggcgg
    19681 aactgaaggt gccgacgaga taaaggacga agatccaacc taccaccgcc tggctcaact
    19741 cgtatggcgc cccgagaagg tggtagccga tgtagttgta cagcgctacg aaaccgccca
    19801 taagcaaggc gcctatggcg tagagtctta ggaggtcccg gtccgtgagg tggccgccga
    19861 gcgaggcgag gaggtgcctg gcgatgagcg gacgcggcct gaagcgcgtg gaagggggga
    19921 gtatgcgcca gaaggcgaag ctcgcgagca cgcacgccag acccagcgag ccgagcgcca
    19981 cgcgccagga gaaagcgtcg gtgaggactc cggacaggat gcgtcccgcc aggccgccga
    20041 tgctgttgcc ggcgatgtac aggcctacgg caaagccggc gtgctccggg tgcacctcct
    20101 cggccagata ggccatcgct accgccggga ggccggccag cgccacgccc tgcaccgccc
    20161 gcagcaccag tagggacggg aagccaggac tcagggccac gaggaccata aggaacgcgg
    20221 acaacaccag ggagacggtc atcatgggga cgcgtcccca tgcctccgag aacgagccgg
    20281 ccagaatgag ggccaccgcg agcgtgccgg tcgccaacga gagcgggagg ctcgccaccg
    20341 ccggcgacac gccgaactcg gccgagaaaa ccggcaacag cggctgcgtg cagtacagca
    20401 tggcgaacgt ggccaggccg gccacgaaga gcgcgaggtt tgcccgccgg aataccggcg
    20461 tcccccggcg cagataaccc ttaccctctt ccaccccgct cgcgtcggga tccacgttcc
    20521 aaagcctcct ccaccgtccg agaattcaac tgccctttcc gagctcgagc aattcttctg
    20581 gcatcctgat gggtttgccg gtggccaggt cgatgcaggc gtggcgtgtg tggccagtga
    20641 cgaggacttc tgcgtcacgg agtactttgt aatcaaagag gagggagacc ttgccgagtt
    20701 cggcgaggtt ggtgatcaaa gcgagctcgt cgtcgaaaaa agccggtctg cggtagtgga
    20761 ggagcgcctt gacgacgacg aggccgcggc cctggcgctc catctccctg taagagtggc
    20821 ctgtggcgcg caaccactcc acgcgcccga cctcgaagta ggagaggtag gtggcgttgt
    20881 tgacgtgccc ctgcgcgtcg atctctgcgt agcggacccg gattttggtc tcgtggacta
    20941 tcttccctcg aaatccggtt tgcgcttctc gccgaaggcg ttcatgcctt cgcgggcgtc
    21001 ttcggtggcg aatagcaagg cgaactggtc ggcctcgaac tcgaggccgc ttaccaggtc
    21061 gacgtcgagg gtgcggttgg cggccgcctt ggcgtgcctc acggcaaggg gtccgttggc
    21121 ggagatctcc gcggccaggt ctctggccgc gttcaacgct tcgccctcgg cgaccacgcg
    21181 gttgacgagg cccatctccc tggcctcccc ggcgcttatg cggcgtccgg tgaagatcaa
    21241 ctctttggcc agggcgggcc cgatcagacg cggcaggcgc tgggtaccgc ccatgccggg
    21301 gagtatcccg aggccgacct cggggaagcc gaaggttgag ttctcggcgg cgatcctgat
    21361 gtcgcaggcg agcgcgacct cgcagcctcc gccgagcgcg tagccgttca cggcggctat
    21421 ggtggggacg gggctcctgt cgagcagggc catcgctgcg tgcccgatct cggagaagcg
    21481 cttggcctcc aggggctcca tgatgctcat ggcccttatg tcggccccgg cgataaaagc
    21541 ccgttccccc gcccctgtga ctataatcgc gcgcggccct tctgactcca gatccagcaa
    21601 cgcctgcccg atctcctcga ccacctgtat gtcaagggcg ttcagcttct cctggcggtc
    21661 gacggtaaga accgcgatat cgtcttcacg ttttacactg acgtaatcca tgtacctccc
    21721 tccggtcggt agcatttcag cacgctccgg ctgacgcctc cgcttgtcag cacttcagca
    21781 tgtcaggttt agcggatgga gacgatcctc aaagagtacg tgctcctctg ggattcactc
    21841 aagcgcatag gagtctctcc acccaccaaa tagctgacat gctgacaagc cgccgcaggc
    21901 ggcgtgctga cttgcttatg tcaagcaggc agtgcctgct tgacatactc tacagctttc
    21961 ttcgtcggtg ttcccggggt gaatacttcg ccgacgccga gctccttaag tttcgtgatg
    22021 tcttctttgg ggatggtgcc gccgacgaag acgaggatgt cttcggcctc gttctctgcg
    22081 agaagctcga ttatccgggg cacgagcgtc atgtgggcgc cggagaggat ggagatgcct
    22141 atggcgtcta cgtcttcctg tatggccgcc tcgacgacct gctccggggt ctggtggagg
    22201 cccgtgtaga taacctccat cccggcatcg cgcagggcgc gggcgatgat cttggccccc
    22261 ctgtcgtgcc cgtcgaggcc aaccttcgct acaaccaccc ttatcgtgcg cgccacagta
    22321 agaccattct aaccggagcc cgaacctcgt gcggcggtgc tcatcttctt agctcgcgca
    22381 ggttgtccca taggacgtag aggccgatca cgagcacggc ggcgaccacg agctgccaga
    22441 agaactcgta gaagatgatg agcgtggcga cgacggcgag tcctatgttg gcgctgctga
    22501 ccaggttgac gaggcggccc ctgaagccgg cttccacgaa cgcgaagaag gcgatgctga
    22561 agacgagcat ggagatgatg tgttcccgct cgaacatggc tatccccacg aagcttatga
    22621 gcatcaggct cacgcttatg gccgcccagg cctcggccac gcggctcacc ctgagctcat
    22681 cctcagaggc cggcttgtga gcgcgggaga tgtgggcgcg ggcctggtcg cgctcaccgg
    22741 ccctcagtcg gtcggcgtac tcggcgaggg actcggatat ggcctcgtct gtggcgagtt
    22801 ttccacgcag tctgtccagc tccctggaga gttcggcgac gcgcctggtc cctgcccggt
    22861 agggcgcgtc gaggtgggac ctgtcgcgca tcgctgcgag ctcggcgccg agcttcttca
    22921 gctgccggct cttgtcttcg atctctgtcc ttaaggtgct gcaccgggag atgatgtcgg
    22981 cccgttgttc gaggatggcc tccagcgtct cggtcggggg agccactttg tccagcccgg
    23041 cccatcccac ggggtcgtac caggcgcggc tgacgctctt gtcgcggttg tacatgggtc
    23101 ctgcgggggc atcctcgcct tcgaaggggt ccctcgcgta gagtccccac agcccgcggt
    23161 agtcagagac ccagtctggg gttgggtccg cgatgagcct gggctcgtcc cacgacctgt
    23221 ctcccccctc tcccacgctc aggccgtctc cgcgcgcgta gtccacgaaa gggatgctga
    23281 agtagcccga cccgccatcg tcctcgccat cccccacgta ctggccgagc c
//
LOCUS       DATMVF010000114         8392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_57267, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000114 DATMVF010000000
VERSION     DATMVF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57267"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VN178_05610"
     CDS             <1..907
                     /locus_tag="VN178_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWS80483.1"
                     /translation="RTTGYAAEEVLGRNCRFLQREDRDQSALGELRTAVREGRHCTVV
                     LRNYRKDGTLFWNELSIYPVRDEDGRLTNFVGVQNDVTERIRAEEILSEIRRAERRRI
                     ARDLHDIVLQDLSGALQSLRLTHLQAKKSGISLDFEEELGALGRASSGLRSAIYDLRH
                     EKERPFLESVESLVELNQQASHEREIRSVVEEGFPVSLPRKQSVELLRILQEALANVR
                     RHSGAKNVEVRLRTDEEAILIEVTDDGRGFDPGSARTGIGLVGMQERVEGLGGNIELR
                     SHPGEGTRVRVRVPVGGGTPDPRRR"
     gene            complement(855..1562)
                     /locus_tag="VN178_05615"
     CDS             complement(855..1562)
                     /locus_tag="VN178_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS80484.1"
                     /translation="MSNESEETIRVAVVDDQRLFTKGISGIVDMLPGVEVVGVAYNGE
                     EAVALCRKEEPNVVLMDISMPKMDGISATREIKDLLPQTAVVILTGHEEDEHVFEGIK
                     AGAQGYLLKDSEPDDLSRAIHTVYAGNTIIAPDLAQKMLTTFEGGRPGGSKHLAAPPL
                     TEREFEVIKALARGMSDRQIAKSLGISEKTVRNHTSNIYRKLHIFDRTQAVIYAVREG
                     VIDVADLEYRPPPEPSP"
     gene            1842..2666
                     /locus_tag="VN178_05620"
     CDS             1842..2666
                     /locus_tag="VN178_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HWS80485.1"
                     /translation="MTLTIGTGPFGDQGDKTFNFEVHPPGDHVLFFEDSPRRVRVMFG
                     GETVADSRRVKLMHEQGHLPVYYFPRGDVRMDLLQATDLTTHCPAKGDASYWSVRIGD
                     DVVENAVWGYPEPIDSAGPLADYLAFYWNKMDHWYEEDEEVFVHPRDPYHRVDVLKSS
                     RHVKVLVDGEIVAETNRPRILFETGLPPRYYFPPEDVLEDVLLPSDKRTECPYKGVAY
                     YYSVRVGPETREAIVWHYPDPIPAAADIEGHLCFFNEKVDLEVDGEAQQRPQTQWS"
     gene            2879..3673
                     /locus_tag="VN178_05625"
     CDS             2879..3673
                     /locus_tag="VN178_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS80486.1"
                     /translation="MAEVNGARIYYEVAGEGEPLVLVHAGIADSRMWEGQLPAFAGRY
                     KVIRHDMRGFGMTAMVDGPFSHHDDLRGLLDSLDVERAHLVGCSMGGGAVLDFALEYP
                     QRVGNLVLVGSVVGGFAPDIDPPRQWDEILAAEEAGDLERVSELEVQVWVDGPGRSPE
                     EVDASVRDLVREMNLIALRNEASGLGDEWAPEPPAADRLPDVQAPTLLIVGDEDQPRT
                     FAAADLLGRELPNVRKVVMHGTAHLPNMERPEEFDRVVLDFLRDHR"
     gene            3790..4899
                     /locus_tag="VN178_05630"
     CDS             3790..4899
                     /locus_tag="VN178_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80487.1"
                     /translation="MAGEDELFQMELNLHPNEASGLMAIGDEELLGEHGWDLGFWACL
                     DEGEIEGCIAAIGHRRGSPEEEGWEIERLRAEASGAAGKTEDAEAITRDEGTGYVYIL
                     GSHFGGKDGPLQPKRGFVARFREADVRHITDHPAMELDVSRRSFALHRLINDALEESG
                     PELIPLGKQSHKALIAATIERGEKEHKKWAGLVREDDYPINIEGAAFRENGTLLLGLR
                     FPTTAEGRPILVELEDIDRLFDPDGALPEVRGFWTADAIGKGGEMAGVRDLALLEAGE
                     GEELHLVTGNVDSRDKQSVLIQDYPGGRETVATHFRCALPKDAHSGSLKAEFVREFPS
                     LPRVEGMAVTPEDRTYYVTDEDEGVHLRLTRLLVG"
     gene            complement(4921..5370)
                     /locus_tag="VN178_05635"
     CDS             complement(4921..5370)
                     /locus_tag="VN178_05635"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWS80488.1"
                     /translation="MADLPQQGESLPDLELTTESGEHIGTGGLTGQKTVLYFYPKNDT
                     PGCTKEACAFRDRMNDYAKADIQVYGVSLDSPESHREFREKYGLNFPLLTDVEGRASQ
                     ALGVLGDRGMARRVTFLLDSEGRISKVYPEVSPETHADEILDDAASL"
     gene            5538..6716
                     /locus_tag="VN178_05640"
     CDS             5538..6716
                     /locus_tag="VN178_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWS80489.1"
                     /translation="MRKKDSAERLRGRRLRRAFYLVLLAVLIGLLVSYWSWVDAQARA
                     VMVISSVLDAPVLTPAAEAVSGEPGFEDFPVAGNPTLVARPAGEGPWPAIFLVNGTVR
                     EGRKLPEVRNLAEGFARAGYLVVVPDLPGLTEGEITPETARETVEVVREVSARPDVED
                     GGVALVGVSTGATLALLAAEKSSLEGRISVVAGIAPYSDIRTVVNIATTGHYRGSDGE
                     LIRYEADSFLPYVMAHSLVAALPPGEDRRTLSGELAGIDREDPDPLDGLRSRGTEDLE
                     PAAASVIRLLANEDPERFEDLYGELPEGVRADLRELSPLAGTGRINVPVELATGPHDK
                     YFPPSQSYALERIAPERRVTVTGALDHAKLEASLGDIPAFATFDAFVVRSLRTARAQE
                     "
     gene            6846..7448
                     /locus_tag="VN178_05645"
     CDS             6846..7448
                     /locus_tag="VN178_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80490.1"
                     /translation="MVPSPSLALRSTATTLLPMVALILLLSPGAWAQDERAKGGISAE
                     AATCPIGVYVTSLRDLDPVGDSFGIDFWVWSVHPQGDGPLDSLEFVNAKQIETRLERT
                     TERGDREWSRLKARATVLHDWDVRDFPFDRQNLTLDLGIAGSDAPACGVDRAGSGYDK
                     GIAPEGWRIAAFDVERHTRENAWLWGPRDGARSSRCHLRA"
     gene            7687..7815
                     /locus_tag="VN178_05650"
     CDS             7687..7815
                     /locus_tag="VN178_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80491.1"
                     /translation="MGVDLFEGLGVAIDELAHRLLIALDEFVYIFDRGHLKITSIV"
     gene            complement(7946..>8392)
                     /locus_tag="VN178_05655"
     CDS             complement(7946..>8392)
                     /locus_tag="VN178_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWS80492.1"
                     /translation="PAAEFNIYVDPEAAREVFESGLPITMSGLDITHQAGAGPDERER
                     LRSTGRVGSIVAGFLDFFAATYERVFGFDDPPLHDPVAVAAVLEPGLLKTRPMRVDIE
                     CESDLTRGETVCDFYGVTGKKPNAEVGVDLDRDGFFDLLHGALRRL"
BASE COUNT         1545 a         2587 c         2733 g         1527 t
ORIGIN      
        1 gaggacgact gggtacgccg ccgaggaggt tttgggccgc aattgccgat tcttgcagcg
       61 cgaagaccgg gaccagtcag cgctcgggga gctcaggacg gctgtccgcg aggggcggca
      121 ctgcaccgtt gtgctcagga actaccgtaa ggacgggaca ctgttctgga acgaactcag
      181 catctacccc gttcgggacg aagatggacg tttgaccaac tttgtcgggg tccagaatga
      241 cgttaccgag cgcatcaggg ccgaggagat tctttcggag atccgtcggg ccgaacggcg
      301 caggatagcc cgggatttgc acgacatagt actgcaggac ctttcgggcg cacttcagtc
      361 gttgaggctt acccacctgc aggccaagaa atccggtata agtctcgact tcgaagagga
      421 actcggggcc ctggggcgtg cgagttcagg cctgcgcagt gccatttacg acctcaggca
      481 cgagaaggag cggccgttct tggagtcggt cgaatcgctg gtggagctca atcagcaagc
      541 ctcacacgag cgcgagatac gctcagtcgt cgaggagggg ttccctgtaa gtctccctag
      601 aaagcagagc gtggagttgt tgcgcattct gcaggaggct ctcgcgaacg taaggcgtca
      661 ttctggggcg aagaacgtcg aggtgaggct gcgcacagat gaggaagcga tcctgataga
      721 ggtcaccgac gatggacggg gattcgatcc tgggtcggcc aggacgggca tcgggctcgt
      781 ggggatgcaa gagcgggtgg aggggttggg tgggaacatc gagctcagga gccaccccgg
      841 tgaaggtacc agggttaggg tgagggttcc ggtggggggc ggtactccag atccgcgacg
      901 tcgatgacgc cttccctcac ggcgtagatc accgcctggg ttctgtcgaa gatgtgcaac
      961 ttcctgtaga tgttggaggt gtggttcctg accgtcttct ccgagatacc caatgacttg
     1021 gctatctgcc tgtcgctcat gccccgggca agggctttga tcacctcgaa ctccctctcg
     1081 gtcagcggcg gagcagcgag atgcttactt ccccctggcc tgccgccctc gaaggtcgtc
     1141 agcatcttct gggcgaggtc gggggcgatt atggtgttac cggcgtagac cgtgtggatc
     1201 gcacgcgaga gatcgtcggg ttctgagtct tttagcagat agccttgcgc gcctgccttt
     1261 atgccctcga agacatgctc gtcttcctcg tggcccgtaa gaatgactac ggcggtctga
     1321 ggcagaaggt ctttgatctc gcgcgtagca ctgatgccat ccattttcgg catggagata
     1381 tccatcagga cgacgtttgg ctcttccttg cggcaaagcg cgacggcctc ctcgccgttg
     1441 taggccacgc ccaccacctc aacgccgggc aacatgtcca cgatgccgga gattcccttg
     1501 gtgaaaagcc gctggtcgtc cacaaccgcc acgcgaatag tctcttcgct ctcgttgctc
     1561 aaaacctcac acccctttgc cgcttcgaag tccagtacga ctctaactgt atccgctgtt
     1621 tgccaagcgc gtcactgatg aacctgtgga gataccgagt gccctgtact tcgaatgatc
     1681 gtctgcgatt gcagcgttat cgtcggatat ttggtccaca ccaggctggg taattctgag
     1741 taggtcaccc gacccatttt gcgcatgtac tataagggcc agaagacgcg ggcctgccgt
     1801 ctgctcttgt cgagctgcgg ccgaaagaga ggaaaggtgc gatgaccctt acgatcggga
     1861 cgggaccgtt cggcgaccag ggcgacaaga cgttcaattt cgaggtgcac ccccccggag
     1921 accacgtact gttcttcgaa gactctccgc gccgggtgcg ggtaatgttc ggcggcgaga
     1981 ccgtggccga cagcaggcgg gtaaagctca tgcacgagca gggacacctg ccggtctact
     2041 acttccccag aggggatgta cggatggacc ttctccaggc cacggacctc acgacgcact
     2101 gcccggcaaa gggcgacgct tcctactggt ctgtgcgtat cggcgacgac gtcgtggaga
     2161 acgccgtgtg gggctaccct gagccgatag actctgccgg gccgctcgcc gattacctgg
     2221 cgttctattg gaacaagatg gatcactggt acgaagaaga cgaggaggtc tttgtccacc
     2281 cccgcgatcc ctatcaccgc gtcgacgtcc tgaagagctc caggcacgtc aaggtcctcg
     2341 tcgatgggga gatcgtggcg gagacgaacc gtcccaggat cctcttcgag accggtctgc
     2401 caccccgcta ctacttcccc cccgaagacg tcttagaaga cgttctactc cctagcgaca
     2461 agagaacgga atgcccttac aagggagttg cgtattacta ctcggtgcgg gtgggccctg
     2521 agaccaggga agcgatcgtc tggcactacc ctgaccccat acccgcagcc gccgacatag
     2581 aaggccacct gtgcttcttt aatgagaagg tggatctgga agtggacggc gaggcgcagc
     2641 agcggccgca gacgcagtgg tcgtagcgaa tcaagcccgc gcctaacctt cctgcgccgc
     2701 aggcgcgggg agcttatact ggccttgcca catcctggca accgatgcgt ctttagagaa
     2761 tatatgccgc cgaaaacagg atagaaccct ggcgccggcg ccaccctgcg cacgggaaca
     2821 gggtgggagg ggccgtagac gtataaggga aagggaagat atgcttcggg agacaggcat
     2881 ggccgaggtc aacggcgcgc ggatctacta cgaggtcgcg ggagaagggg aaccgctggt
     2941 cctggtacac gcggggatcg cggacagcag aatgtgggaa ggccaactcc cggccttcgc
     3001 cggccgctac aaggtcatcc gccacgacat gcggggcttc gggatgaccg cgatggtgga
     3061 tggaccgttc tcccaccacg atgacctgcg cgggctgctc gactccctgg acgtcgagcg
     3121 ggcgcacctc gtgggctgct cgatgggtgg cggggccgtg ctcgacttcg cgctcgaata
     3181 cccgcagagg gtaggtaacc tcgtgctcgt ggggtctgtc gttggcggtt tcgcccctga
     3241 catcgacccc ccaagacaat gggacgagat cctcgctgct gaagaggctg gggacctgga
     3301 gcgggtctcc gagctggagg tccaggtctg ggtcgacggc cccgggagat cccccgaaga
     3361 agtagacgcc tctgtcaggg acctagtcag ggagatgaac ctgatcgccc tcagaaacga
     3421 agcctccggg ctgggcgacg agtgggcacc ggaaccgccc gcggcggaca ggctccccga
     3481 cgtccaggcc ccgaccttgc tgatagtcgg agacgaggac caaccgcgca ccttcgccgc
     3541 ggccgacctg ctcgggaggg agctaccgaa cgtgcgcaag gtcgtcatgc acggcacggc
     3601 acacctgccc aacatggaaa ggcccgaaga gttcgaccga gtcgtgctcg atttcctgag
     3661 agatcaccgc taaggccggc aaccggacgt ccctacaagc ccactatgcc agggctcact
     3721 caccaaggga ggaactttct ggcggctggc gcgtagtagg tgggcgtgtc agggacgagg
     3781 aggtggcgta tggccggtga agacgagctt ttccagatgg aactcaacct gcacccgaac
     3841 gaggcgtcag ggctcatggc gatcggggac gaagagttgc tcggggagca cggttgggac
     3901 ctcgggttct gggcgtgcct ggatgagggt gagatcgaag gctgtatcgc cgccatcggt
     3961 caccgtcgcg gctcccccga ggaggagggc tgggagatag agcggctgcg cgccgaggct
     4021 agcggagccg cgggcaagac ggaggacgcc gaggcgatca cccgcgacga gggcacgggc
     4081 tacgtctaca tcctcggctc ccacttcggc ggcaaggacg ggcccctcca gcccaaacgc
     4141 ggcttcgtcg cccgctttcg ggaggccgac gtccggcaca ttacagatca ccccgccatg
     4201 gagctcgacg tctcccgccg gagcttcgcc ctccatcgcc tcatcaacga cgccctcgag
     4261 gagagtggcc ccgagctgat cccgctcggc aagcagtcgc acaaagcgct gatcgcggcg
     4321 acgatcgaac gcggtgagaa ggaacacaag aaatgggccg gcctcgtgcg cgaggacgac
     4381 tacccgatca acatagaagg cgcggccttc agggagaacg gtactctcct cctcggcctc
     4441 cgcttcccca caacggcaga aggacgtccg atcctggtag agctggaaga catcgaccgt
     4501 ctcttcgacc cggacggcgc cctcccggaa gtccggggtt tctggacggc cgatgcgatc
     4561 ggcaagggcg gcgagatggc cggcgttcgc gacctcgcct tgctcgaggc gggagaaggc
     4621 gaagagctac acctcgtaac aggcaacgtc gacagccgtg acaaacagag cgtcctgatc
     4681 caggactacc cgggcggcag ggagaccgtg gccacccact tccgctgcgc acttcccaag
     4741 gacgcccatt ccggttccct aaaggccgag ttcgtccgcg agtttccgag cctaccgcgc
     4801 gtcgagggca tggccgtcac accggaagac cggacctact acgtcacgga cgaggacgaa
     4861 ggcgtgcatc tgcgccttac tcgactcctg gtgggctgaa tcgcgtcccc accccggatg
     4921 ctacagtgag gcggcgtcat ccaggatctc gtcggcgtgg gtctcggggg agacttcggg
     4981 gtagaccttg gagatcctac cttcagagtc gagcaggaag gtgacgcgtc tggccatgcc
     5041 ccggtcgccc aggaccccga gagcctggga agcgcggcct tctacgtcgg tgagtagtgg
     5101 gaagttcagg ccgtacttct cgcggaactc gcggtgagat tcgggcgagt cgagggagac
     5161 gccgtagacc tgtatatccg ccttcgcgta gtcgttcatg cggtccctga aggcgcaggc
     5221 ttctttcgtg cagcccgggg tatcgttctt ggggtagaag tagaggacgg tcttctggcc
     5281 cgttagccca cccgtaccga tgtgctcccc gctctcagtc gtgagctcca ggtcgggcag
     5341 tgactcgccc tgctgtggta ggtctgccat gaaacgcctc cttctcgttg atatgtgccc
     5401 gtaagtctac gaagtcttac ccgggctggt gcaaaagaaa tccatccgga tagcagtact
     5461 tgcgacgtgt tgtggacagg cggtggtcca cgttgcatag ttatgggtgg atgaggtcgt
     5521 ctctggcggg agaggcgttg cggaaaaaag attcggcaga aagactccgc ggtagaaggc
     5581 tccggcgcgc cttctatctc gtgttgctcg cggtcctgat cgggttgctc gtctcgtact
     5641 ggtcgtgggt ggacgcgcag gcgcgcgccg tcatggtgat ctcctccgtt ctcgacgcgc
     5701 cggtattgac gcccgcggcc gaagcggtga gcggtgagcc cggtttcgag gacttccccg
     5761 tggccggcaa ccccactctc gtcgcgaggc ctgcgggaga gggaccgtgg ccggccatat
     5821 tcctggtcaa cgggaccgtg cgcgagggaa ggaaactgcc cgaggtgcgg aacctggccg
     5881 agggcttcgc gcgggccggc tacctcgtgg tcgtgcccga cctgccaggg ctgacggagg
     5941 gtgagatcac ccccgagacc gcccgcgaga ccgtcgaggt cgtgcgcgag gtctccgcgc
     6001 gaccggatgt cgaagacgga ggggtggccc tggtcggggt ctcgaccggc gccacgttgg
     6061 ccctcctcgc ggcggagaag tcgagcctcg aagggcggat ctcggtcgtc gccggcatcg
     6121 cgccttactc ggacatccgc accgtcgtga acattgcgac gaccggccac taccgcgggt
     6181 ccgacggaga gcttatccgc tacgaggcag actccttcct cccttacgtc atggcccact
     6241 ccctcgtcgc cgcgctgccg cccggcgaag accggcgtac gctgtcgggc gagctcgcgg
     6301 gcatcgaccg cgaggaccct gacccgctcg atggtctacg ctcgcgcggg acggaagacc
     6361 tggagcctgc ggccgcgagc gtaatccggc tgctcgccaa cgaagacccg gagcgcttcg
     6421 aggacctcta cggggagctg cctgaggggg tgcgcgccga tctcagggag ctctcgccgc
     6481 tcgccggcac gggacggata aatgtcccgg tcgagctcgc caccggtccc cacgacaagt
     6541 acttccctcc ctcacaatcg tacgcgctag agcgcatcgc gcccgagcgc agggtgacgg
     6601 taacaggggc gctcgaccac gccaaactcg aagcctcact aggagatatt ccggccttcg
     6661 caacgttcga cgccttcgtg gtccgctccc tgcgcacagc ccgcgcccaa gagtaacaat
     6721 tcagcacgct caggctttta gccttcgcct gtcagcacgc ttggggcctt tcggcccccg
     6781 ctctcggctt gtcagtctct ccgctggagc aaaccgtcca atcccgccgg ttgtagaatg
     6841 cgcagatggt cccgtctcct agcctcgcgc tccggagcac tgccactacc ctcttgccga
     6901 tggtcgcctt gatcttgctc ctctcgccgg gggcgtgggc ccaagacgag cgggcgaaag
     6961 gcgggatctc cgccgaagcg gcaacttgcc cgatcggggt ttacgtcacg tctttgcgag
     7021 acttggaccc cgtgggcgat tcgttcggta tcgacttctg ggtctggagc gtccaccctc
     7081 agggggacgg ccctctggac agcctggagt tcgtaaacgc caagcagatc gaaactcgcc
     7141 tggagcggac gaccgagcgg ggcgaccggg aatggtccag gttgaaggcc agggcgaccg
     7201 tgctccacga ctgggacgtg agggacttcc ccttcgaccg gcagaacctc acgttggacc
     7261 tcggcatcgc cggctccgac gccccggcgt gcggggtaga ccgcgccggc tccggctacg
     7321 ataagggcat cgcgccggaa gggtggcgga tcgccgcctt cgacgtcgag cggcataccc
     7381 gcgagaacgc gtggttatgg ggtccgcgtg acggtgcacg atcttccagg tgccatcttc
     7441 gggcctgaat atgcttgtga cgcgcaaagt cacggggctg atatcctcac cgccgccaac
     7501 cctggccctg tagcgctcca tctccacgat acaggcaagc tcgggggtga cgtacttcgc
     7561 cacgttctcg aagcctaaga tctcgccgtc cctgtagttg gatgcggaac cctctatggc
     7621 ccgcgcggac ccatcccatc cgcgcacggg agggccgaag gggttggcga ggcttgcgtc
     7681 gtctcgatgg gagtagatct ctttgagggg ctcggggttg ccattgacga gctcgcgcat
     7741 cgcttgttga tagcgctcga tgagttcgtc tacatcttcg accgcggcca tctgaagatc
     7801 acctccatcg tgtagaaacc ccgtccttcc ccaaggacgc tctctgagta cgtcctacgc
     7861 ctatttaccg cagcgcgtgg agaggctgta tgagaagtag tcctcggatg gttgggtact
     7921 ctagcatact gaggggcaaa ccatcctaca gccggcgcag cgccccgtgc agcaagtcga
     7981 agaaaccatc gcggtcgaga tcgacgccga cctcggcgtt gggttttttg cccgtcacac
     8041 cgtagaagtc gcagacggtc tcgccgcggg tgaggtcgct ctcgcactcg atatcgaccc
     8101 gcatcggacg tgtcttcagc aggcctggtt cgagtaccgc cgctacggcg acgggatcgt
     8161 gcagcggcgg gtcgtcgaag ccgaataccc tctcgtaggt ggcggcgaaa aagtccagga
     8221 atccggctac tatgcttccc accctacctg ttgaccgcaa ccgctccctt tcgtcaggtc
     8281 cggcgccggc ctggtgtgtt atgtcgaggc ccgacatcgt tatgggcagc cctgactcga
     8341 agacctcacg ggccgcctcg gggtcgacgt agatgttgaa ctcggcggcc gg
//
LOCUS       DATMVF010000115        12796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_157484, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000115 DATMVF010000000
VERSION     DATMVF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157484"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(566..2767)
                     /locus_tag="VN178_05660"
     CDS             complement(566..2767)
                     /locus_tag="VN178_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS80493.1"
                     /translation="MTRLLTLTGAGETGKTRLAQEVASDLVGAYPDGVWLVQLAGLGE
                     PELVAQVVAGTLGVREQPGRPLGATLVQELRAKHLLLVVDNCEHLIEAVARFTNELLD
                     ACPKLRILATSREPLRVPGEVVRRVPSLPVPEPEDPTLLAEGGLARLAVTRLFLDRAH
                     WRKEEIVSSGRSVQTVVGVCRRLEGIPLAIELAAARTATLSVEQIEERLGDSLGLLTT
                     GFRSADLRHRTLRATLDWSHALLSETERVLFGRLSVFAGGWTLEAAEALGSGVGEGEV
                     LDPLSLLVDKSLVALEEGEHGAMRYTMLEPVRQYARQMLRESGEEETVQGRHADYFLA
                     LAEEAEPQMSGPNQEAWLRRLEREHAHLRGALAWALDPSDRRESREHSTGLGLRLAGA
                     LGYFWAVYGPGEGLGSLEKGLKRGRAGSKPALAKALYEAGWIALWQGESDRTIALLEE
                     ALTLFRELEDRRGVATSITKLGAALLHRGDMEGATPLREEAEALRGEPLDRWVLAWLT
                     TFLAAAALFEGDYERSGALGTEGLAVYRELGDKRGIVMCHVGLGLPELAKDDHEQAAL
                     LFERTLRLLRGPGEKSAVAYALLGLAGADAARGEPAFAGRLWGAAKALREEIGATLSH
                     WDLYGYDYEGRVSAARNMLGDEGTWERAWTEGRAMTPDEAIEYALGTREPAPPTALTS
                     RMSSLVEAPDDPAPSASGRWRSWWGVGSPTARSPHSSTSQSERSTTTSATS"
     gene            complement(3134..3712)
                     /locus_tag="VN178_05665"
     CDS             complement(3134..3712)
                     /locus_tag="VN178_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80494.1"
                     /translation="MASFSSRAPTRREERSGRGPEAVRVWLLGGFRLSVGSRTIGEER
                     WRRRKAPALVKLLALAPGHKLHRKWVTDLLWPELDVKAATNNLHQILHHARRTLEPAG
                     EPAGATRYLLLRAEQLALCPEAPLWVDVEAFGEASAGARRAREPAAYRAAIELYAGEL
                     LPEDRFEGWAEQWREELRGTYLDLLVELAALH"
     gene            4239..7532
                     /gene="smc"
                     /locus_tag="VN178_05670"
     CDS             4239..7532
                     /gene="smc"
                     /locus_tag="VN178_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWS80495.1"
                     /translation="MLSAIYIKGFKTFARPVRMPLEGGITSIVGPNGSGKSNITDAVL
                     FAIGEQSPGVLRAGAMSELIFSGSETLSGASAAEVTLVLDNEDGRISLPYGEVSISRR
                     ISRSGETEYRINGSRARLADVRAVAGEAGLGRHSILRQGAVDAIVAGGAAACRLALEE
                     AAGLGVYRRRRFSASRRLEKADIQLERSRQLEAELEVQLRRIEREAAAARAYREIESR
                     YRELSLAHLYRIATRGLDGLRGRFEEARTQVEDLAARERDVREEEAGIESRLQRLEEG
                     LGKTEQRLEDLEDSAEELRVEALRSDRTLLRLEAARGREGETRLAVSRLEDELRRVSQ
                     TLAVLEDRSRDLEAELSERREESDRLQKVVARSSAKSASAERERARLARELEGLRTRL
                     AGLGSHGVQPVMSEEDLSHLGAVISELEEAEDQATMGHLRGLRDEVTGMVARVDVNAE
                     EVSRRRGALAAAVGRAESRVRALKETAQDGASTRLYEVIRARPGYEVAVEAALGEYGA
                     GVLAENLDEGMKLLSDAEPVAVRLDARAMESNGGLPGKPLIECVEVRDARYDEAVRRL
                     LTGIFVVERPGDGANANGYVAVTKDGFRLTRTGVSLRPGPGNFAREARLAAWLKHLEA
                     LENGPGETLYDLRETVSDVSNRLENLAGTVGKVTGLADRTARARALLSRDAKRRKTAG
                     ESSRRSFLERQESLSTLSDDAARTENALAEAEKDAERSREELAAATAAAESAAAATAD
                     SDRRLSELRATLASGQSRREAVSRRLDRSANTKDEGAGNAEALAAKAAKAATQLVTTV
                     RRRRHALRRLRGESAEAHRRVSAERTTLSRRTVDLAGKLATARAHAERLAEEVSRAES
                     AAAEAGEEIRAEWGATLDAARQEAEKHEESTDAERQTLARKLKRFGDVNLLALSQEEG
                     LRERHRFVSAQRADAEDAANELNRIIRSVDGEIESRFSETFGRVRDAFGEMVPRMMEG
                     SSGVLDLSDEGVEIGLRLGRKGWRPLRVLSGGERALLALSFLFSVFLSRPGGDSGAFC
                     VLDEAEAALDDLNLARFLTVVDSHRANGQYLLVTHQKRTMAAADVLYGVIQDASGATT
                     VVSKRLQGE"
     gene            7543..8748
                     /gene="ftsY"
                     /locus_tag="VN178_05675"
     CDS             7543..8748
                     /gene="ftsY"
                     /locus_tag="VN178_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWS80496.1"
                     /translation="MARSWRSFFRRNTDEEQENGPAVAGEPEDSTEDVEEGNGRLDAD
                     LESLVEVPPAEEVQAERKEPTPEDVWEIEETEPEESLPEPGAVDEAPEAIAERQESGG
                     WFGRLRQGLSRSRASFVGQLNAAVAEFRDVEDEEFWERVEEILISSDVGVPTTAKLVA
                     ELEQEALERNIKSGAELRELMIEHATRMLEGPVELDVSHEPTVILMVGVNGTGKTTTI
                     GKLSRFLPGTKMFAAGDTFRAAAIEQLQQWGERTDTPVIARERGADAAAVAHEAVHAA
                     RRDGTDVLLIDTAGRLHTKVNLMAELDKVQRVVQRQLPGAPHEVLMTVDATTGQNGLR
                     QAQMFKEVAGLTGIILTKLDGTAKGGIALAIANEIGVPIKLISVGERVEDLHPFDARE
                     FAEALFGDL"
     gene            8749..10080
                     /gene="ffh"
                     /locus_tag="VN178_05680"
     CDS             8749..10080
                     /gene="ffh"
                     /locus_tag="VN178_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWS80497.1"
                     /translation="MFDTLGERLNKALDGVRGSGNLTEDQVKKVTREIRLALLEADVN
                     YGVVKEFVSNVRERATGAEVSKALSPGQQVVKIVDEELANLMGGTAHKLSFASRPPTV
                     VMLAGLNGHGKTTAAGKLALFVRKLGKNPYLVACDTYRPAAIEQLQQIGRELSVPVYT
                     EGTGPKPEEIAERGIREARDGGYDFVIVDTAGRQVVDMEMMEEIRRVRKASRPHSVLL
                     VLDVVTGQNAVEVAQAFQEYADFDGMVLTKLDGDARGGVALSVVSVTGRPVKFASEGE
                     KMAEFDYFYPDRMAGRILGMGDVLTLIEKAEQQMDRTEAEAQGKKLMSGKFTFEDFLQ
                     QMQMIKKMGPLSGLLGMIPGLGKQLRDVEIDDRQLGRVEAMITSMTVQERRNPKLLQN
                     PSRARRIARGSGATQQDVQKLVKQFKEMQKMMAQMGKGGGLPRIPGLSGRR"
     gene            10099..10341
                     /gene="rpsP"
                     /locus_tag="VN178_05685"
     CDS             10099..10341
                     /gene="rpsP"
                     /locus_tag="VN178_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWS80498.1"
                     /translation="MAVKIRLARHGSKGNPFYRIVVADSRSPRDGRFIERIGTYDPQQ
                     EPSDIKVDEEKAREWLGKGAQPTDQVRNLLKISGVL"
     gene            10342..10581
                     /locus_tag="VN178_05690"
     CDS             10342..10581
                     /locus_tag="VN178_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWS80499.1"
                     /translation="MAQLENLLLVLARALVDEPDKVEVSGTESDSRVDLELRVAPEDM
                     GRVIGRQGRTIRAIRTVVKAASVKAGKRVTVEVPD"
     gene            10608..11084
                     /gene="rimM"
                     /locus_tag="VN178_05695"
     CDS             10608..11084
                     /gene="rimM"
                     /locus_tag="VN178_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWS80500.1"
                     /translation="MAIGVVVTPHGVRGTLRVRALGPGKHLREGKEPLVAGVRRRISS
                     ARRTPKGFLLNLEGVESRTDAQRLRGEQLLLDRGELDATEEGEFYVADLLGLTAVSDA
                     GEVLGTVTDTFETAAHEVLVVRYDRAGQELYLPFTLEHVPRVDLQTGRIVARPPEG"
     gene            11088..11780
                     /gene="trmD"
                     /locus_tag="VN178_05700"
     CDS             11088..11780
                     /gene="trmD"
                     /locus_tag="VN178_05700"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWS80501.1"
                     /translation="MRSIDVFCVFPDAVDAALGVGVVGRAIENGIVGYRSFDLRAYSP
                     DGRIDDMPYGGGAGMVVRVDVVARALEEVYGVPAREVRTGRRVVLTEPAGRRLTQGYV
                     DEVASGPDLTVICGRYGGIDERVWSTLATEAVSLGDFVLSGGEIVAAALADAAIRRLH
                     GVLGNAESLVGESFSAEDVVGPPTYTRPASWDGEQVPGVLLSGDHAKIRAWREHEARE
                     RTRARTTGKWSP"
     gene            11814..12140
                     /gene="rplS"
                     /locus_tag="VN178_05705"
     CDS             11814..12140
                     /gene="rplS"
                     /locus_tag="VN178_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWS80502.1"
                     /translation="MITSDNLQQQDVTFKPGDTVRVHYRVIEGNRERVQVFEGLCISR
                     KGSGIHETFTVRKNSFGVNVERIFPLHSPKIAKIEVASRGAVRRAKLYYIRDKVGKKA
                     RVKKAK"
     gene            12154..12780
                     /locus_tag="VN178_05710"
     CDS             12154..12780
                     /locus_tag="VN178_05710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWS80503.1"
                     /translation="MSGTHSTGPPPGESSLYAFDAAWVLGRRGPLAGADEAGRGALAG
                     PLVAAAVVLGDAEVAGINDSKLLRPSVREEIFGALLRRAESVSVVAFPAWWIDRHGIG
                     LANREALRRALDSLKSCAGSSLADGNLALGPDVECLPRADSKSAAVAAASVVAKVLRD
                     DAMRALSETHTGYGFGRNSGYGTPEHRLALSELGHCRVHRLSYVGVGA"
BASE COUNT         2424 a         3963 c         4298 g         2111 t
ORIGIN      
        1 cgtagagtgc ggtgaggaag gtgtccacat cgagcatgag gggtcggttc ctttcccttg
       61 gggatcgggc agcaagccga ctttagttcg tgggcacctt ccgctcaaat tgagcgcaga
      121 taattcacac caagcgttta agagacccta cactccttgc tcgcacctca accgcagcga
      181 gatagtagga gggcgggtag ctggccccgg tctagaagga gggcaggtag acgtcctgcg
      241 ccctcacgtg aggtctccct aacacgtgat ggacgcagca tactccccta agaagaaccg
      301 gcacaatctc ttgtcctggt gcgtccaaga aatgggtata aatgcctacg tttcgagccc
      361 tccagctaag agccagctaa gagccgtgta tcgaatgcga tcgttactct attggacgcg
      421 tccacgtgga cgccttgcgg cccgtgggca aggaggaatc tagaaaccca atagcccctc
      481 tacggaaatt gcgaggattg cggcgatgag tcccaccgcg ccgcccagac ggcgatctgg
      541 gccctcgaac gcaaccccaa cttgctcagg atgttgcgga cgtggttgtg gaccgttcgc
      601 tctgagaggt ggagctgtga ggagatctgg cggttggtga gcccacgccc caccaagagc
      661 gccacctccc gctcgcgctc ggtgcgggat cgtcgggcgc ttcgacaaga gacgacatgc
      721 gtgaagtgag agccgtgggc ggggccggct cccttgtgcc caaagcgtac tctatggctt
      781 catcgggcgt catcgccctc ccctccgtcc aagccctttc ccaggtgccc tcatcgccaa
      841 gcatgttgcg tgcggcgctc acccgacctt cgtagtcgta gccgtacagg tcccagtgcg
      901 aaagggtggc gccgatttct tcgcgtaggg ctttcgcggc cccccacagc cttcccgcga
      961 aagctggttc tcctcgcgcg gcgtctgccc ctgccaagcc caagagggcg taagcgacgg
     1021 ccgacttctc tcctgggcca cgcagcaggc gcagggtcct ttcgaacagc agcgccgctt
     1081 gttcgtgatc gtcctttgcc agctcgggga gccccaagcc tacgtggcac atgacgatgc
     1141 cccgcttgtc ccccagctct cggtagacgg cgaggccctc tgtgccaagc gcaccggatc
     1201 gctcgtaatc gccctcgaac aacgcggccg ccgccaggaa ggtggtcaac caagcaagca
     1261 cccaccggtc gagaggctct ccccgcaacg cttcggcttc ctcgcgtagt ggggtcgcac
     1321 cctccatatc gccccggtgt agcagcgctg cccccaattt ggtaatcgag gtggcgacgc
     1381 cccgtctatc ctccagctct ctaaacagcg tgagggcttc ctcgagcagg gcgatcgttc
     1441 tgtcggactc gccctgccag agcgctatcc agcctgcttc gtagagggcc tttgccagag
     1501 cgggtttgga tcccgcccta ccccttttga gacccttttc caacgacccg agcccttcac
     1561 ccggaccgta cacggcccag aaataaccca gcgcgcccgc cagccttaaa cccagcccgg
     1621 tgctgtgctc cctcgattct ctcctatccg acgggtcaag cgcccaagcg agggcacctc
     1681 gcagatgggc gtgctccctc tccagcctcc tcagccacgc ctcctggttc ggccccgaca
     1741 tctggggctc ggcctcttcg gccagggcga gaaagtagtc ggcgtggcgc ccctggacgg
     1801 tctcttcttc gccgctctcc ctcaacatct ggcgggcgta ctggcgcacg ggctcaagca
     1861 tcgtgtagcg catagcgcca tgctcgccct cctcgagggc taccaacgac ttgtccacca
     1921 gcagcgagag aggatccaag acctcaccct ccccaacgcc cgatcctagt gcttcggcag
     1981 cctccagtgt ccagccgccc gcgaacaccg aaaggcgacc gaacaagacc cgctcggtct
     2041 cggagagcaa ggcgtggctc cagtccaagg tcgccctgag cgtccggtgg cgaagatccg
     2101 cgctccgaaa gcccgttgtc aagagcccca gggagtcccc aagcctctcc tcgatctgct
     2161 ccacggagag cgtcgccgtc cttgcggccg caagctctat ggccagcggg atgccctcca
     2221 gcctccggca gacgcccacc accgtctgga cgctcctccc ggaggacaca atctcctcct
     2281 tccgccaatg cgccctgtcg aggaacagcc gcgtcaccgc caagcgcgcc aaccctccct
     2341 cggcaagcag tgtgggatcc tcgggctcgg gcacgggaag cgagggcacc cgccgcacca
     2401 cctctccggg cacgcgcagc ggctcgcggc tggtggccag gatgcgcagc ttcgggcagg
     2461 catcaagcaa ctcattcgta aagcgcgcca cggcctcgat caggtgctca cagttgtcca
     2521 caacgagcag aagatgcttc gcccgcagct cctgaactag cgtggcgcca agcgggcgac
     2581 ccggctgctc tcgcaccccg agcgtccctg ccaccacctg cgccactaac tcgggctctc
     2641 cgagcccagc cagttgcacg agccaaactc catccgggta ggctcccacg aggtcacttg
     2701 caacctcctg agctagccgc gtcttgcccg tctctcctgc ccccgtgagc gtcaagagcc
     2761 gcgtcatcgc tagctcacgc ttgagctcta ccctctcacg ctcgcgatct acgaagctgg
     2821 tcctagtggc tggcaggttg tgccgaccaa cgctcggatg tgcctccgac gtcgaacctc
     2881 ccaatgccac agacgagcgc ggcaacctgc cgaccgctat ctcgtcgcgg aggcggcggc
     2941 tagcctggtg gggtttcctg ccgaagccac cagatagggc cttctcgagc cgctcgtact
     3001 gcagtaaagc ctcttgaggg cgcccagcca aggcgtacag gcacattagt cccgcgtgtg
     3061 cctcttcctt aggtggctct tgggcaacta cggtccgcaa cgcctcaata gcgggctcgt
     3121 actcagcacg ctcctagtgc agcgcagcca gttcaacaag caaatcgagg taggtcccgc
     3181 gtagctcctc tcgccactgc tcggcccacc cctcaaagcg atcttcgggc agcaactcac
     3241 cggcgtacag ctcgatcgcc gcccggtagg cagcaggttc gcgggcacgt cgagcgcccg
     3301 ccgaggcctc cccgaaggcc tccacgtcca cccacagcgg tgcctcggga cacagcgcaa
     3361 gctgttctgc ccgcaggagg aggtatcgag tagcgccagc tggttcgccg gcaggctcta
     3421 gggttcgacg agcatggtgg agtatctggt ggaggttgtt ggtggccgct ttgacgtcca
     3481 gctccggcca taagaggtcc gtcacccact tccgatgcag tttgtgcccc ggcgcgaggg
     3541 ccagcagttt gacgagtgcc ggcgccttcc tccgccgcca cctctcctcg ccgatggtcc
     3601 gcgagcccac cgatagccgg aagcctccga gcaaccatac gcgtacggct tcgggtcccc
     3661 tccccgagcg ctcctcgcgt cgcgttggtg ctctagagct gaaagatgcc acctcccgct
     3721 ctcacctagt gtggatactt tcccaagctt gtcgctttac tcacctactc ggccagtatc
     3781 caagcacaat gcgatgaatc ctcaatgaat ccggaatgga ggccagaaaa gcgggcacca
     3841 gatgacccat agtctccggt cctcgatggc tgtgcatacc ggtggctcct caagcgatac
     3901 gaccatgacg gaggcccgac gacatactct ggctcgccct tgatcgccat gactctgtca
     3961 ggctcagacg aggggtacgt gcgaaggcaa gagttcgcat tctgcgatct cgacggaggg
     4021 agcctctaca cgatctacga agacgcctgc gagcccggcg agcctgagga atgtttgcgg
     4081 ttctgatgag cccggacttt gtggtggggt ttgtggcgtg ggtaggttct ggctgactga
     4141 caagccgccc gcaggacggc gagctgactt gctgatagct attttgctgc acttttctac
     4201 gtgcttttcg tgttgaagtt gcgtaaaata cactttcagt gctctcggcg atctatatca
     4261 aggggttcaa gaccttcgcc aggccggtca ggatgccgct ggaagggggc atcacttcga
     4321 tcgtcggtcc gaacggttcg gggaagagca acatcaccga cgccgtgctc ttcgcgatcg
     4381 gggagcagag tcccggggtg ttgcgggccg gggcgatgag cgagttgatc ttctccggct
     4441 cggagacgct ttcgggtgcg agcgccgccg aagtaaccct cgtgctggac aacgaagacg
     4501 gccggatctc gctcccttac ggggaggtct cgatctcgcg ccggatctcc cgcagtgggg
     4561 agacggagta caggatcaac ggctcccgcg cccgtcttgc ggacgtacgg gccgtcgccg
     4621 gcgaggcggg actaggtcgc cacagcatac tgcgccaggg cgccgtggac gccatagtcg
     4681 ccggtggggc ggccgcgtgc cggctcgcac tggaagaggc cgctgggctc ggggtctaca
     4741 ggaggcgacg cttctcggcc agccgccgtc tggagaaggc agacatccag ctggaacgaa
     4801 gccgccagtt ggaagccgag ctcgaagtcc agttgcgcag aatagaaagg gaggccgccg
     4861 ccgcccgagc gtaccgggag atcgaatccc gctaccgcga actctcgctg gcgcacctct
     4921 accggatcgc aaccaggggg ttggacgggc tcagaggaag gttcgaggaa gccaggacgc
     4981 aggtcgagga tctggctgcg cgcgaacgtg acgttcgcga agaagaggca gggatcgaaa
     5041 gccgcctgca gcggctcgag gaggggctcg gaaagaccga acagcgactc gaagacttgg
     5101 aggatagcgc agaggaattg cgggtggagg ccctgcgctc ggacaggact ctgctgcgac
     5161 tggaggcggc ccggggacgc gagggggaga cgcggctcgc cgtctcccgc ctggaagacg
     5221 agttacgccg ggtgtcccaa actctggccg ttctcgaaga taggtccaga gacctggagg
     5281 ccgagttatc ggagaggcga gaagagtcag accgcttaca gaaggtcgtt gcgagatcca
     5341 gcgcgaagag cgcgtctgcc gagcgtgaga gggccaggct ggccagggaa ctggagggat
     5401 tgcgcacgcg cctcgccggg cttgggtccc acggtgtaca gcccgtgatg tccgaagagg
     5461 acctctcgca ccttggggcc gtgatctcgg aactcgaaga ggcggaagac caagcgacga
     5521 tgggtcatct tcgcgggctg cgcgacgagg ttaccggcat ggtcgcgcgg gtcgatgtga
     5581 acgccgagga ggtcagccgc aggcgcgggg ccctcgccgc cgctgtgggg agggccgaat
     5641 cccgggtacg cgccttgaag gagacggccc aggacggagc gagcacgcgg ctctacgagg
     5701 tgatccgggc gcgtcctggc tacgaggtcg ccgtggaagc ggccctcgga gagtacgggg
     5761 ccggggtgct cgcggagaac ctcgacgagg ggatgaagct tctgtcggac gccgagccgg
     5821 tggccgtacg cctcgacgcg agggccatgg aatccaacgg gggcttaccc ggaaaacccc
     5881 tgatcgaatg cgtagaggta agagacgcgc gctacgacga agcggtacgg cgcctcctga
     5941 ccggcatctt cgtcgtggag cgtcccggtg acggggcgaa cgccaacggg tacgtggccg
     6001 ttacgaagga cggttttcgc ctcacgagaa cgggcgtgag cctgcggccc ggccccggta
     6061 acttcgcccg cgaggcgcgt ctcgccgcct ggttgaaaca cctcgaagct ctggagaacg
     6121 ggcccggcga gaccctctac gacctgaggg agaccgtctc ggatgtttcg aatcgcctgg
     6181 agaacctcgc cggaaccgta ggcaaggtga cgggtctcgc cgaccggacg gcgcgcgccc
     6241 gggccctgct ctcacgggac gcaaaacgcc ggaagaccgc aggggagagt tccaggagat
     6301 cattcctgga gcggcaagag tcgctctcta ccctctccga cgatgctgct cgcacggaga
     6361 atgcgcttgc ggaagccgag aaggatgcgg agcgttcgag ggaggaactg gccgccgcca
     6421 ccgccgccgc cgagtcggcc gccgcggcga ccgccgactc ggaccggagg ctgtcggagt
     6481 tgcgcgctac cctggcctcc ggccaaagtc gcagggaggc ggtctcgcgc aggctggatc
     6541 gctcagcgaa cactaaagac gaaggagcag gcaacgctga ggcgttagcc gcaaaggcgg
     6601 cgaaggcggc tacacagctc gttacgaccg tccgccggcg caggcatgca ttgcggcgcc
     6661 tccggggaga gtccgccgag gcgcaccggc gggtctctgc agagcgcacg acgctctcgc
     6721 gcaggaccgt ggatctcgcg ggcaagctgg ccaccgcccg ggcccacgcc gaacgcctcg
     6781 ccgaagaggt gtccagggcc gaatccgcgg cggccgaagc cggcgaggag atacgggcgg
     6841 agtggggcgc caccttagac gcggcccgcc aggaggccga gaagcacgag gagagcaccg
     6901 acgcagaacg ccaaacgctc gcccgaaaac tgaagcgctt tggggacgta aaccttctcg
     6961 ccctttccca ggaggaggga ctgcgcgaac gccaccgttt cgtctcggcc cagagggccg
     7021 acgcggagga tgcggccaac gaactcaacc gcataatacg atcggttgac ggggagatag
     7081 agtcgcgttt ctcggagacc ttcgggaggg taagggatgc gttcggggag atggtgccgc
     7141 ggatgatgga aggatcttct ggggtcctgg atctctccga tgagggggtg gagatcgggt
     7201 tgcggctggg aaggaagggc tggaggccgt tgcgcgtcct gtccgggggc gagcgggctt
     7261 tgctcgcgct gtctttcctg ttcagcgtgt ttctaagccg gccaggcggg gattcggggg
     7321 ctttctgcgt gctcgatgag gctgaggcgg cgctcgacga tctcaacctg gcccgctttc
     7381 tcactgtggt agactctcac cgggctaacg gccagtatct gctggtcacc catcagaaac
     7441 gaacgatggc cgcggcggat gtgctctacg gggtgattca ggatgcctcc ggggccacca
     7501 ctgttgtgtc gaagcgtttg cagggagagt aagaagtcgc ctatggcgcg ctcgtggcga
     7561 agttttttca gacgaaacac agatgaagaa caagagaacg gcccggcggt agccggcgaa
     7621 ccggaagatt ccaccgaaga tgtcgaagaa ggtaacggcc gcctcgatgc ggatctcgaa
     7681 tcgctcgtag aggtacctcc ggccgaggag gtgcaggccg agaggaagga gcctactccc
     7741 gaagacgttt gggagatcga ggagaccgaa cccgaggaat cgttgcccga gcccggcgcg
     7801 gtcgatgagg caccagaggc gatcgccgag cggcaggagt ctggcgggtg gtttggcagg
     7861 ctgaggcagg gtctcagccg cagccgcgcg tcgttcgtcg gacagctcaa cgcggctgtt
     7921 gcggagttca gggacgtcga agacgaggag ttctgggaga gagtcgagga gattttgatc
     7981 tcctccgacg taggggtacc gactacggcg aagctcgtcg ctgagctcga gcaggaggcg
     8041 ctggagagga acatcaagag cggtgccgag ttgcgggagc tcatgatcga gcacgccaca
     8101 cgtatgctcg aagggcccgt cgaactcgac gtctcgcacg agcccacggt gatcctgatg
     8161 gtcggcgtga acgggacggg caagacgacg accataggca agctctcgcg tttcttgccg
     8221 gggaccaaga tgttcgccgc aggggacacg ttcagggccg ctgctatcga gcagctccag
     8281 cagtggggcg agaggacgga cactcccgtg atcgcgcgcg agcgcggcgc cgacgccgcc
     8341 gccgtcgccc acgaagcggt gcatgccgcc aggcgcgatg gtaccgacgt gctgctcatc
     8401 gacacggcgg gcaggcttca taccaaggtc aaccttatgg ccgagctgga taaagtacag
     8461 agggtcgtac agcgtcagct tccgggggcg ccgcacgagg tgttgatgac cgtcgacgcg
     8521 accacgggtc agaacgggtt gcggcaggcc cagatgttca aagaggtcgc cggactcacc
     8581 gggataatcc tcaccaagct cgacgggacg gccaagggcg gcatcgccct cgccatagcc
     8641 aacgagatcg gggtgccgat caagctgatc tcggtcggtg agagggtcga ggacctccac
     8701 ccgttcgacg cgcgcgagtt cgcagaagca ttgtttggag atctttagat gttcgatacg
     8761 ttaggcgaaa ggctcaacaa agccctcgac ggggtccgcg gtagcggcaa cctcaccgag
     8821 gaccaggtaa agaaggttac gcgtgagatc cggctggccc tcctcgaggc cgacgtgaac
     8881 tacggcgtcg tcaaggagtt cgtctccaac gtccgcgagc gggccacggg ggccgaggtc
     8941 tcgaaggccc tcagccccgg ccagcaggtc gtcaagatag tcgacgagga gcttgcgaac
     9001 ctgatgggag gcacagctca caagctctcc ttcgcctcca ggccgcccac ggtcgtgatg
     9061 ctcgctggtc tgaatggaca cggcaagacg acggcggccg gcaaactcgc gctgttcgta
     9121 cgcaagctcg ggaagaaccc ttatctggtc gcctgcgaca cttacaggcc ggccgcgatc
     9181 gagcagctgc agcagatcgg gcgcgagctc tctgttcccg tgtacacaga gggcacgggt
     9241 ccgaagccgg aggagatcgc cgagcgcggc atcagggagg ccagagacgg cgggtacgac
     9301 ttcgtgatcg tcgacaccgc gggccggcag gtagtcgaca tggagatgat ggaggagatc
     9361 cggcgcgtca ggaaggcctc gaggccgcac tccgtcctcc tggtgctcga cgtcgtgacc
     9421 ggccagaacg ccgtcgaagt ggcgcaggcc ttccaggagt acgccgactt cgacgggatg
     9481 gtcctgacca agctcgacgg cgacgcccgc ggtggggtcg cgctctctgt ggtctccgtg
     9541 acgggtcggc ccgtcaagtt cgcctcagag ggcgagaaga tggccgagtt cgattacttc
     9601 tacccggaca ggatggccgg ccgcatcctc gggatgggtg acgtccttac gctcatcgag
     9661 aaggccgaac agcagatgga ccgtaccgag gccgaggcgc agggcaagaa gctcatgagc
     9721 ggcaagttca ctttcgaaga cttcttgcag cagatgcaga tgataaagaa gatgggtccg
     9781 ctatctgggc tgctcggtat gatccctggc ctgggaaagc aactcaggga cgtggagatc
     9841 gacgacaggc agctcggcag ggtcgaagcc atgataacct cgatgaccgt ccaggagcgc
     9901 aggaacccca agttgttgca gaacccgagc cgggcgcgta ggattgcgcg gggctctggc
     9961 gcgacccagc aggacgtcca gaagctcgtc aagcagttca aggagatgca gaagatgatg
    10021 gcccagatgg gcaagggcgg cggattgccg cgcataccgg gtctgtcggg gcgtagatag
    10081 agttcgagag gagatggcat ggcggtaaag attagactcg cgagacacgg ctccaaaggc
    10141 aaccccttct acaggatagt ggtggccgac tccaggagcc ctcgcgacgg ccggtttata
    10201 gagcggatcg ggacctacga cccgcagcag gaaccttccg atatcaaggt cgacgaagag
    10261 aaggccaggg agtggctcgg caagggggcg cagcctaccg accaggtgcg caacctgctg
    10321 aagatctccg gggtcctcta ggtggcgcag ctagagaacc tgttgctcgt cctggcccgc
    10381 gcactcgtgg acgaaccgga taaggtcgag gtctcgggga ccgagtcgga ctcgagggtg
    10441 gatctcgaac tgcgggtggc gcccgaggac atggggcgtg tgatcggccg ccagggacgg
    10501 acgatacggg cgatccgcac ggtagtaaaa gccgcatccg tgaaggccgg aaagcgggtc
    10561 accgtggagg tcccggacta gagaccgtgg aggatctctc cgatcccgtg gcgatcggag
    10621 tcgtggtcac accgcacggg gtccgcggca cgctgagggt tcgtgccctc ggtccgggca
    10681 agcacctcag ggaaggcaag gagccgttag tcgcaggcgt aagacgacgc atctcttccg
    10741 ccaggcgaac cccaaaaggt ttcttgctca acctggaagg cgtagagagc aggaccgacg
    10801 cgcaacgcct gagaggcgaa cagctcttgc tcgaccgcgg agaactcgac gcgaccgagg
    10861 agggggaatt ctacgtggcc gacctcctcg gccttacggc cgtgagcgac gcaggggaag
    10921 tcctcggaac ggtgaccgat accttcgaga ccgccgccca cgaagtgctc gtggttcgtt
    10981 acgacagagc cgggcaggaa ctctatctgc cttttaccct ggagcacgtg ccccgggtgg
    11041 atctgcagac ggggcggatc gtggcccgtc cgccggaagg ctaagagttg agaagcatag
    11101 atgtcttctg tgtcttcccc gatgcggtcg acgcagcctt gggagtaggg gtcgtcggca
    11161 gggccataga gaacggaatc gtcggctacc gcagcttcga cctgcgcgcc tactctccgg
    11221 atggccgcat cgatgacatg ccgtacggtg gcggcgccgg gatggtcgtc agggtcgatg
    11281 tcgtggccag ggccctcgag gaggtctacg gggttcctgc gagggaggtg agaacggggc
    11341 ggcgggtcgt ccttacggag ccggcaggac ggaggttgac acagggctac gtcgatgagg
    11401 tggcttccgg accggatctt acggtcatct gcggacgcta cggcggtata gacgagcggg
    11461 tttggagcac cctcgccacg gaggccgtat cgctgggtga tttcgtgttg tccgggggtg
    11521 agatcgtggc cgccgcgctg gccgatgcgg cgatccggcg tctacacggc gtcctcggca
    11581 acgccgaaag cctcgtggga gagtcgttct cggccgagga tgtggtcggc cctcctacct
    11641 atacgcggcc tgccagctgg gatggagagc aggtgcctgg cgtgctatta tcgggcgatc
    11701 acgcaaagat ccgcgcctgg cgcgagcacg aggcgcgaga gcgtacacgc gcaaggacga
    11761 ccgggaagtg gtcgccctga ccctccggag aaagtccatg agaggagaag aagatgataa
    11821 cgagcgataa tttgcagcag caggacgtca cgttcaagcc gggagatact gtacgggtcc
    11881 actaccgtgt tatcgagggc aacagggagc gtgtgcaggt cttcgagggc ctgtgtatct
    11941 cgcgcaaggg cagcgggatt cacgagacat ttacggtcag gaagaactcc ttcggggtca
    12001 acgtggagag gatcttcccg ctccactccc cgaagatcgc caagatcgaa gtcgcctctc
    12061 gtggcgccgt aagacgggcg aagctttact acatccgcga caaggtgggc aagaaagcca
    12121 gggtcaagaa ggccaagtaa gcaggccaga gcgttgtcgg ggactcattc caccggccct
    12181 ccgccagggg agagttctct ctacgctttc gacgcggcat gggtgctggg tcgccgcggg
    12241 ccactggccg gtgccgatga ggcaggacgt ggggcactgg cgggaccact ggtggccgcg
    12301 gccgtcgttc tgggtgacgc cgaggtcgcc gggatcaacg actccaaact tctgaggcca
    12361 tctgtgcggg aggagatctt cggcgcgctg ctccgcaggg ccgagtccgt ctcggtagta
    12421 gccttcccgg cctggtggat cgatcgccac ggcatcggcc tggcgaaccg cgaagccctg
    12481 agaagggctc tcgactcgct gaaatcctgc gccgggagca gcctcgcgga cggcaacctg
    12541 gcgctcggcc ctgacgtgga gtgcctgccg agggccgaca gcaagagcgc ggcggtcgcg
    12601 gctgcgagcg tggtggccaa ggtcctccgg gacgacgcaa tgcgtgccct ctctgagacc
    12661 cacaccggct acgggttcgg acgcaacagc gggtacggga cgcccgagca ccggctcgcc
    12721 ctctccgaac tcggacactg ccgggttcat cgtctcagtt acgtcggagt cggagcctga
    12781 aagacaaatc ccccgg
//
LOCUS       DATMVF010000116        14989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_111158, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000116 DATMVF010000000
VERSION     DATMVF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14989
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111158"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="tyrS"
                     /locus_tag="VN178_05715"
     CDS             complement(<1..965)
                     /gene="tyrS"
                     /locus_tag="VN178_05715"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWS80504.1"
                     /translation="MKATPEHQTIYRNAVDVIPREELDRRLSGGESLRVKLGIDPTAP
                     DIHLGFAVVLTELRAFQDLGHTAVLVIGDYTARVGDPSGRSKTRPILSPGQIEENTRT
                     YLEQAYIILDRDRTEVRHNSEWLAPLTMADIIRLTRATTLARILERDDFSKRYAANDQ
                     ITLTELLYPLMQAYDSVAIEADVELGGTDQLYNLLMGRQVMEYYGKRPQCVVTTPLLV
                     GTDGKIKMSKSVGNYIGVTDPPGEMFGKVMSIPDSLMHDYYSLLLDRPILEGEPVGQK
                     RELARSLVSKFHGEGDAAAAEKHFDAVVRREVPDDAPVVRLPEDE"
     gene            1129..3321
                     /locus_tag="VN178_05720"
     CDS             1129..3321
                     /locus_tag="VN178_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWS80505.1"
                     /translation="MVSKILRGTGIFLLCVLASVMAFAGGGYLGLIEGVRELDEPRTY
                     ETHPTYIYSAPLSGNEDSRRVIGTIFEGQNRKTASLSEMPPYLLDAVVAKEDERFREH
                     GGVDLWGIMRALWVDIRAGEAVEGASTITQQYVKNAYLSHDRSISRKVKEALIAIEVE
                     RKQDSKDQIIADYLNTVYFGNNAYGVEAAAETYFDKSVEDLSIAESATLVGLLWSPST
                     LGEDRDGAQHQRDLVLMKMFDTGYVPSREYNRALEEPLPGAWPANAPTKSGFTGPELT
                     RDFAQYTRDELIRRYGVNTVLEGDLSVYTTIDLEEQLAAREVAYGPSGYLPDADDPDL
                     ALVSIEPETGRIRTMVGDRNENSQFNLVTQGRRQPGSSFKPFALIAALEQGIDPDTKY
                     DSEEKEYEVDVGTGKPETWRVQNYDGIERGEISLQEALWQSDNTVFADLAMNAGGRGL
                     KDGPEEIADVAKRCGITTRLPVHPRPSIVLGAYEVSPLDMASAYATIANHGRRVEPIA
                     ITEVVSHEDDNDEKVLYVAPEHPEGEQVIAEDVANEAIGIMVGDVTEGIAKDASLGDR
                     PVAGKTGTSENFFDAWFIGFTPEVVTGTWMGYAEGGQTLEYILDYARKLHGLSGGINP
                     AEIWKTYTQTVLSGEPIEKLDGVVIPERADSSADPKPHEKNDSGATRRRAAASPEARR
                     VVPEPRRVTAASASASSVSPSRAGGPRPKRAGATRYSSASSSASAAPR"
     gene            complement(3302..3976)
                     /locus_tag="VN178_05725"
     CDS             complement(3302..3976)
                     /locus_tag="VN178_05725"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWS80506.1"
                     /translation="MFHSLRGRARPRRTLPPYNRPYGRAGISQDVLKGAFYDRDPRQV
                     AVDLLGCVLSHDSPEGRAAGVIVETEAYRPEDPACHAYKGPTMRNRNIFGPPGIAYVY
                     LSYGTHNLLNVVCEPEGVGSAVLIRALRPSDGEDLMERRRGRGRDLCNGPGRLTQALG
                     VDLSYDGQDLTSGSLTISEGDAPPEGIIETTRIGITRGTDLPWRYLVEGDKFVSVPPK
                     AISGRR"
     gene            complement(3880..4239)
                     /locus_tag="VN178_05730"
     CDS             complement(3880..4239)
                     /locus_tag="VN178_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80507.1"
                     /translation="MKVIADRIVTLGFGKYVRADNIVALVPIEEDRGSGRRTLVYVDG
                     LPEPLVAGRTEGTILRDMVGAESGAAELLRELQLQIGQVRPLLKSSIRSEAGLDLDEL
                     SRRITDLTGEQASPKMF"
     gene            4368..4985
                     /locus_tag="VN178_05735"
     CDS             4368..4985
                     /locus_tag="VN178_05735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWS80508.1"
                     /translation="MWAVANRAWRIPAKPGIFMAVRLDDRARTLEGRRIELRRHAREN
                     YHLYAEWYGDPEIWHLTSWAPSPLSRSAVERLFEDRELSRTDDSFAIHIKYEDEPIGV
                     ISLANKSEANESADLSVIVGHRDDRHQGYGTEAIDRLLRYAFEELGLNRIGLSVFDFN
                     EEAISAYEKLGFVEEGRIRQAIKRHSGFHDAILMSILKSEWNPNS"
     gene            complement(4975..7407)
                     /gene="pheT"
                     /locus_tag="VN178_05740"
     CDS             complement(4975..7407)
                     /gene="pheT"
                     /locus_tag="VN178_05740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWS80509.1"
                     /translation="MRVPLSWLHEYVDFDLSAEDLVDLFSLRSQEIDGVYRLGVMDGE
                     VVVGEVIEFGPHPNADRLFVAKVDLGGREVQIVAGAPNPYPGARVSVVLPGSVMSDGT
                     KLREAKLRGLGSYGMMMSERELGISADHGGILLLDDSYEVGRPVADYFPVGETVLDID
                     VTPNRPDLWGMIGVARELAAILGTDYRVPEPTFEAGGKPTHDFGLRVEAEDLCPRYDL
                     RRISGLRPGQEAPLWMRRRIHAAGMRPINAIVDSTNYVMLETGQPIHAFDAARVREGI
                     VVRRGRPQEKITLLDGSTRSLDGETLVIADEERGLVIAGIMGAEDAEVGEETTDALIE
                     VANFEGRNILQTSQKLGLRTDASGRFERGLDPNMVAYAMDRVSGLLTQAPGGTPADDT
                     LSHYPEPVEPWRVPMRLGRAELLLGMPVRADEAAERLEALGCEVEREDGRLWATVPTF
                     RRDLRREADLVEEVGRLIGLDRVPERLPAVPQAGALTAAQLKTRLIRRLLADLGLSEA
                     ITYPFGPERWRDDLGLEDGAVRLENPLSAEAGFLRTSMLPGLLDATARNRAFGARGGA
                     LFELGSVFFADVPQGDLREAALRFRMTGEAGAHDASGLMGVREEGRIGIVLAGTVRPA
                     GWNVPEVEAGFFEAKGLVVRLVPGARFAPAAREYLHPGRSAEVLVGEKVAGWVGELHP
                     DVAGRFGLRGWSVSALELDSAFCNPEPEPRFEPFVNVPAVARDLAVLVDERVPVGDMV
                     RLIQDSPRPLLTEVRVFDVYTGSPVPVGRKSVAFGLTFQGEKTLTDEEVDAEVGRILG
                     NLEEAFDARVRS"
     gene            complement(7420..8448)
                     /gene="pheS"
                     /locus_tag="VN178_05745"
     CDS             complement(7420..8448)
                     /gene="pheS"
                     /locus_tag="VN178_05745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWS80510.1"
                     /translation="MTVSERIEQLKTEALSAVSSSASTADLEDVRVRFLGRSAELTEI
                     KKSIGSLLPEERKEVGRSSNLASLEIEAALGSRTQEVAAREREERLRAEAVDVTLPGV
                     PFQRGHLHPSQIVIDDVVDFFVGLGYRVAEGPEVETDYYNFTALGIPPGHPARTMQDT
                     FFVDDGLVLRTQTSPMQIRTMLAQEPPVYVVCPGRTYRRDSDPTHTPMFHQVEGLAVD
                     RGLTLGHLKGTLAAMARHVFGDDVEIRLRPSYFQFTEPSVEMDMSCFACGGRDSNCKV
                     CKGAGWLEMLGAGMVDPGVLEEVGYDPEEYTGYAFGMGPDRMAMVKYGVPDLRLFFEG
                     DLRFLRQF"
     gene            complement(8458..9144)
                     /locus_tag="VN178_05750"
     CDS             complement(8458..9144)
                     /locus_tag="VN178_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWS80511.1"
                     /translation="MPDTPAISLKRAARLKTKKHREAERLFLAEGGAILKEAPTAPIQ
                     VFDSAEQLRRVSALTTPSGPVGVFPYLDATATELLASVRRVVLLHRIQDPGNVGTAIR
                     SAHAFGAGVALSRGCADLYNPKTVRATMGSIFHTPVARELESLGFLNEAGSAGFGLVA
                     AVPEAKDTPESLPRGRLVICIGAEGSGLPEEIVGACEMEVGIPSLAPSLNASVAASIL
                     LYEAYSRVLP"
     gene            complement(9152..9775)
                     /gene="rplT"
                     /locus_tag="VN178_05755"
     CDS             complement(9152..9775)
                     /gene="rplT"
                     /locus_tag="VN178_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWS80512.1"
                     /translation="MARTARSVHARKKRRKVLEQAKGYRGTKHTSYKRAKEQVWKSGV
                     YAYEGRKQRKRDFRALWIQRINAGAREHDLSYSKLIHGLRLAEVDLDRKVLADLAATE
                     PEIFGAIVAQAKNALDGKPVARRVEVERSTEPEQPVSSTVRAPEAPAEQVEETEEVKT
                     SEDEIEATDAASRKAEELNVDLSSVEGTGQGGRITVGDVAKAAKGDA"
     gene            complement(9777..9974)
                     /gene="rpmI"
                     /locus_tag="VN178_05760"
     CDS             complement(9777..9974)
                     /gene="rpmI"
                     /locus_tag="VN178_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWS80513.1"
                     /translation="MPKMKTHKGAAGRFEVRKKGRLKRWRAGHNHMLEKKSRKQKRRL
                     NTETDLTRSDEKKLKRLLGER"
     gene            complement(10084..10752)
                     /gene="infC"
                     /locus_tag="VN178_05765"
     CDS             complement(10084..10752)
                     /gene="infC"
                     /locus_tag="VN178_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWS80514.1"
                     /translation="MGSASVRRTSKSIVRGGLTAPKARIREEWCAVATEEEPRINGQI
                     RAREVRLISADGEQLGIKHIREAMDYGERADLDLVEVAPNANPPVVRMMDYGKFKYQK
                     EQARKAARKKQVNVNVREIKLRPKIGDHDFNTKRGHVERFLRGGDKVKVTIMFRGREV
                     QHPELGERLLRRLASDLDGLGRIESQPNLDGRNMVMVMAPKKESSQAEASQTSGRRER
                     AARR"
     gene            complement(10901..12814)
                     /gene="thrS"
                     /locus_tag="VN178_05770"
     CDS             complement(10901..12814)
                     /gene="thrS"
                     /locus_tag="VN178_05770"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWS80515.1"
                     /translation="MASVKLPDGRQLEIEPGEKAREVVEKIGKRLARDAVVAKLDGQL
                     IDLDAPLNGGGDFEVVTIDSYEGLEVLRHSTAHAMAQAIVELYPGSKLTIGPPIENGF
                     FYDIEVAGRLTEEDLPRIEERMREIVARDLPIQREEVSKAEAEDLYVDNPYKTEIIEG
                     LEDGEISVYKQGDFFDLCSGPHVPSTGRLGAFKLQNIAGAYWRGDEKNPMLTRVYGTA
                     WPTEKELKAYLRRLEEARARDHRKIGKDLELFAFSPDTGAGIPLFLPKGEMMRHLMED
                     YVREVQTRHGYEHVWTGHLVNEGLFAKSGHLEHYRESMFPPMIDGETRYRLKPMNCPS
                     HMTLYNSRAHSYRDLPLRYAEFATLYRYEKSGELTGLTRVRSLTQDDAHVFCTEDQIQ
                     EEFARALEIVREVLDTYGFDEYRVQLSLRDQDVAKYVADDAKWSRAEKELREALDTAA
                     IAYDEEAGEAAFYGPKADFVAKDVLGREWQLSTIQVDFIQPARLGCEYIGEDGKAHTP
                     VLLHRAVTGSTERFMAVLIEHYAGAFPVWLSPVQAVVIPVADRHLGYAREVKKQLAER
                     DLRVEVDDSQNSMQKKIRDNSRQKIPYLLIVGDREAEQGSVNVRKRGEKQQEEMALDG
                     FAEAVLGEVRSRT"
     gene            complement(13313..13660)
                     /locus_tag="VN178_05775"
     CDS             complement(13313..13660)
                     /locus_tag="VN178_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80516.1"
                     /translation="MRRTALLLAAMTVALVVVSGVALAKTYVGTENGEKIVGTKFNDH
                     INGLSGDDVLLGYAGPDKIRGGDDNDQQYGGRGNDIIYSEGGFRDLVNCGRGTDTAYV
                     DSKDRVVDCERKR"
     gene            complement(14018..14158)
                     /locus_tag="VN178_05780"
                     /pseudo
     CDS             complement(14018..14158)
                     /locus_tag="VN178_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(14538..>14989)
                     /locus_tag="VN178_05785"
     CDS             complement(14538..>14989)
                     /locus_tag="VN178_05785"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80517.1"
                     /translation="ACSDAGALLNLTQWDYTGPVQGFKDGESVDLGEHRLRFIETPHV
                     HHWDSMMVFEETSGSLFPADLYMQWGDQPPIVREDLGGEMCQGYRESGIFAAEEPVLR
                     VVDRLEKLDPQWIHPMHGGSLPKRWCPTLLVRSEKRSSPSKGRYSGG"
BASE COUNT         2722 a         4894 c         4457 g         2916 t
ORIGIN      
        1 tcctcgtcct caggcaaccg gaccacgggc gcatcgtccg gcacctcgcg gcgcaccacg
       61 gcatcgaagt gcttctcggc ggcggccgcg tcaccctccc cgtggaactt gctcaccagt
      121 gagcgcgcca gctcgcgttt ctgcccaacg ggctcgccct cgagtatagg cctgtcgaga
      181 agcaaagagt aatagtcgtg catcaaagag tcggggatgc tcatgacctt cccgaacatc
      241 tcgcccggcg ggtcagttac gcctatatag ttgccgacgg acttgctcat cttgatcttg
      301 ccgtcggtgc cgaccaggag cggggtcgta acgacacact ggggtcgctt cccgtagtac
      361 tccatcacct gccgacccat gagcaaattg tagagctggt ccgtgccgcc gagctcgacg
      421 tcggcttcga tcgccacgga atcataggcc tgcatcagcg gatagagtag ctcggtcaac
      481 gtgatctggt cgttcgcggc gtagcgcttg ctgaagtcgt cgcgctccaa aatgcgggcc
      541 agggtcgtcg ccctggtcag ccttataata tccgccatcg tcagcggcgc gagccactcc
      601 gagttgtggc ggacctcggt ccgatcgcgg tcgaggatga tatacgcctg ctcgaggtag
      661 gtccgcgtgt tctcctcgat ctgccccggc gagaggatag gacgcgtctt cgaacgcccg
      721 gaaggatcgc cgacgcgcgc cgtatagtcc ccgataacca gtaccgcagt gtgccccaaa
      781 tcctggaagg cacggagctc cgtcagtacc acagcgaagc cgagatgtat gtcaggggcc
      841 gtggggtcga tgccaagttt tacccgcaaa ctctcgccac cagagaggcg gcggtcgagc
      901 tcctcccgtg ggatcacatc tacggcgttc ctgtagatcg tttggtgctc tggtgtagcc
      961 ttcattaaca gatctcaacc ttctcgtaat aagaatgtaa cttgcgtcac cctagtgtag
     1021 tataccggcc ccatgtccgg cacccaccag aagagaacga ggcggaccaa ggacgtgcgc
     1081 cggccggtac gtctacccga gggccgcagg gaaactcccc gcaggagcat ggtctcgaag
     1141 attctgcggg gcacgggcat ctttcttctg tgcgtgctgg cctcggtgat ggccttcgcg
     1201 ggtggcgggt atttggggct gatagagggc gtgcgggagc tcgacgagcc gcggacctac
     1261 gagacccatc ccacctacat ctactcggca cccttaagcg gcaacgagga ctcgcggcgg
     1321 gtgatcggga ccatattcga gggccagaac cgcaagacgg cctcgttatc ggagatgcca
     1381 ccgtacctgc tcgacgccgt ggtcgccaaa gaagacgagc gtttcaggga gcacggcggc
     1441 gtcgacctct gggggatcat gcgcgcgctc tgggtcgaca tcagggccgg ggaggcggtc
     1501 gagggtgcca gcaccatcac acagcagtac gtaaaaaacg cctacctctc gcacgaccgt
     1561 tcgatctcca ggaaggtaaa agaggccctt atcgccatag aggtggagcg caaacaggac
     1621 tcgaaggacc agatcatcgc cgactacctg aataccgtct acttcggtaa caatgcctac
     1681 ggggtcgagg cggccgccga aacctacttc gacaagtcgg tcgaggatct gagcatcgcg
     1741 gagtcagcga ccctggtcgg gctcctgtgg tctccgtcga ccctgggaga ggaccgcgat
     1801 ggggcgcagc atcagcggga cctcgtgctg atgaagatgt tcgatacggg ctatgttccg
     1861 agccgggaat acaaccgggc cctcgaggag ccactgcccg gggcttggcc agccaacgcg
     1921 ccaaccaagt cggggttcac agggcctgag ctcacccgcg acttcgcgca gtacacgcgt
     1981 gacgagctca tacggcgtta cggtgtgaat accgttctcg aaggcgacct cagcgtctac
     2041 acgaccatag acctggagga acaactagcc gctcgcgagg tcgcctacgg gccttccggg
     2101 tacctccccg atgctgacga ccccgacctc gccctcgtct ccatagagcc agagacgggc
     2161 cggataagga ccatggtcgg cgacaggaac gagaactccc aattcaatct cgttacccag
     2221 ggcaggcgcc agcctggcag ttccttcaag ccgttcgccc tcatcgcggc cctggagcaa
     2281 ggcatcgatc ccgataccaa atacgactcg gaggagaaag agtacgaggt cgacgtcggc
     2341 acgggcaaac cggagacgtg gagggtacag aactacgacg gaatcgagcg gggggagatc
     2401 agcctgcaag aggccctctg gcagtcggac aacacggtgt tcgcggacct ggcgatgaac
     2461 gccggtggcc ggggtctgaa agacggcccg gaggagatcg cggacgtcgc aaagagatgc
     2521 gggatcacca cgcgtctccc cgtacatccc aggccttcca tcgtgctcgg cgcgtacgag
     2581 gtctcccccc tcgacatggc atcggcctac gcgactattg cgaatcacgg acgcagggtg
     2641 gaaccgatag cgatcaccga ggtagtcagc cacgaggacg acaacgatga gaaggtactc
     2701 tatgtggcgc ccgagcatcc cgagggggag caggttatag ccgaggacgt cgccaacgag
     2761 gcgatcggga tcatggtggg agacgtcaca gaaggtattg cgaaggacgc ttcactagga
     2821 gaccgtccgg tcgccggcaa gacgggtacg agcgagaact tcttcgacgc ctggtttata
     2881 ggtttcactc ccgaggtggt taccggaacg tggatgggct acgcagaagg cggacagacc
     2941 ctcgaataca tcctcgacta cgcccgcaaa ctacatggac tctcaggcgg aatcaacccg
     3001 gccgagatct ggaagacgta tacgcaaacg gtcttgagcg gtgaacccat cgaaaagctc
     3061 gacggcgtcg tgataccgga aagagccgac tccagcgcgg accccaaacc ccacgaaaag
     3121 aatgattccg gtgccacccg tagacgcgcg gccgcgagtc cggaagcccg aagggtcgtc
     3181 ccagaacccc gtcgcgtaac ggcggcgagc gcctccgcat ccagtgtttc cccgtcgaga
     3241 gccggagggc cgcggcccaa gcgcgccgga gccacccgct acagctccgc atcttccagc
     3301 gctagcgccg ccccgagata gctttcggtg gtacggagac gaatttgtcg ccctcgacca
     3361 gataacgcca cggaaggtct gtcccgcgtg taatgccgat cctggtggtc tcgatgatgc
     3421 cctcgggcgg cgcgtctccc tccgagatag tcaggctacc cgaggtgaga tcttgcccgt
     3481 cgtaagacag atctactccc agtgcctggg tcaggcggcc aggaccgttg cacaggtcac
     3541 gcccgcgacc ccgacgccgc tccatcagat cctcgccgtc ggagggacgt aacgccctga
     3601 tcaagacggc gctccccacg ccctcgggct cgcagacgac gttgagcagg ttgtgcgtgc
     3661 cgtaggagag gtatacgtag gcgatgccgg gcggaccgaa gatattacgg ttgcgcatcg
     3721 tcggcccttt gtaagcgtgg catgcagggt cctcgggacg gtacgcttcc gtctccacga
     3781 tcacgcccgc cgcacgtcct tcgggggagt cgtgcgagag gacgcagccg aggaggtcga
     3841 ccgcgacctg cctgggatcc ctgtcgtaga aagcgccctt cagaacatct tgggagatgc
     3901 ctgctctccc gtaaggtctg ttatacggcg ggagagttcg tcgaggtcga gcccggcctc
     3961 ggagcgaatg gaagatttca gcaaaggacg gacctgcccg atctggagtt gcaactcgcg
     4021 gaggagctcc gccgccccgc tctcggcgcc gaccatgtcc ctgaggatcg tgccctccgt
     4081 ccgtccggcg acgagtggct cgggcagacc gtcgacgtag acgagcgtgc ggcgtccgct
     4141 cccgcggtcc tcttcgatcg ggacgagggc cacgatgttg tcggccctca cgtattttcc
     4201 gaacccgagg gtgacgatac ggtccgctat aaccttcatc tcgatcctat ctccatcgta
     4261 cagaccctta taacatcccc gccggctccg ggcgacccgt cgcgccgaca aagaccacca
     4321 aagtctaccg gacggcgcag accgaaagct ctcccgagac ggagaacgtg tgggcagtag
     4381 caaaccgggc gtggcgcatc cccgccaaac ccggtatatt catggccgtg cggcttgacg
     4441 acagagccag aacgctggaa ggtaggagga tcgagcttcg ccgccacgcc cgcgagaact
     4501 atcacctcta cgcggagtgg tacggggacc cggagatctg gcacctgacg agttgggcgc
     4561 cttcgcccct gagccggtcg gcggtggaga ggttgttcga ggacagggag ctctccagga
     4621 cagacgactc tttcgccatc cacatcaagt atgaggatga gcccataggt gtgatcagcc
     4681 tcgcgaataa gagcgaggcc aatgagtcgg cggacctctc ggtgatcgtt ggtcaccggg
     4741 acgaccgcca ccaggggtat gggacggagg ccatcgatcg gctgctgcgc tacgccttcg
     4801 aggagctcgg gctaaaccgc atcggcctta gcgtcttcga cttcaacgag gaggccatct
     4861 cggcctacga gaaactagga ttcgtggaag agggacgcat ccgccaggcc ataaagcgcc
     4921 actccggttt tcacgacgcc atcctcatga gcatcctgaa gtcggagtgg aaccctaact
     4981 cctgacgcgt gcatcgaacg cttcttcgag gttgccgagg atacgcccga cctcggcgtc
     5041 cacctcttcg tccgtgaggg tcttctcacc ctgaaacgtg aggccgaagg ccacactctt
     5101 tcgtcctact ggaacgggac ttcccgtgta aacgtcgaag acgcgtacct cggtcagcaa
     5161 gggacgtggc gagtcctgaa tcaggcgcac catgtcgccc accggtaccc gctcgtcgac
     5221 gaggacggcc aggtccctgg cgacggcagg gacgtttacg aaaggctcga atcggggttc
     5281 tggttccgga ttgcaaaacg ccgagtccaa ctccagggcg gagacggacc aacctcgcaa
     5341 tccgaacctt cctgcaacgt cgggatgtag ctccccgacc catccagcca ctttctcacc
     5401 gaccaacacc tctgccgagc gtccaggatg cagatactct ctcgccgccg gcgcgaaacg
     5461 tgcgccaggc accagacgca cgaccagacc cttcgcctcg aagaagccgg cttcgacctc
     5521 tggcacgttc caaccggcag ggcgcacggt gccggcgaga acgatcccga ttctgccttc
     5581 ctccctcaca cccatgaggc cgctggcgtc gtgcgcgccc gcctcgcccg tcatgcggaa
     5641 ccgcagcgcg gcctcccgca agtcgccttg cggaacgtcg gcgaaaaaca cgctccctag
     5701 ctcgaacagc gccccgcctc gcgccccgaa cgccctgtta cgggccgttg cgtcgagtag
     5761 tccaggcaac atactggtcc gcagaaagcc ggcctcggcg ctaaggggat tctctaacct
     5821 gacggcccca tcttccaggc cgaggtcatc cctccagcgc tccgggccga acgggtaggt
     5881 aatagcctcc gagagtccga ggtccgcgag cagacgcctg atcagacgcg tcttcagctg
     5941 cgcggccgtc agcgccccgg cttgcggcac ggcgggcagc ctctccggca cccggtcgag
     6001 cccgatgagc cggccgacct cctcgacgag atctgcctca cgacgtagat ccctcctgaa
     6061 ggtgggaacg gtggcccaca accttccgtc ttcccgctcc acctcgcacc ccagggcctc
     6121 cagacgttcg gccgcctcat ccgcccgcac gggcatgccg agaaggagct cggcccgccc
     6181 tagccgcata ggtacacgcc agggctccac tggctcgggg tagtggctga gggtgtcatc
     6241 ggcgggcgtt ccacctggag cttgcgtgag caggccactc acccggtcca tcgcgtacgc
     6301 caccatgttc ggatcgaggc cgcgttcgaa gcggcctgag gcatcggtgc gcaaaccaag
     6361 tttttgggaa gtctggagaa tattgcgccc ttcgaagttg gcgacctcta tcagggcgtc
     6421 cgtggtctcc tcccctactt ctgcgtcctc ggcgcccata atccctgcga tcacgagccc
     6481 gcgctcttcg tcggcgatca cgagcgtctc cccatcgagg gagcgcgtgg agccatcgag
     6541 aagtgtgatc ttctcctggg gcctgccgcg cctgacgacg atgccttcac gcaccctggc
     6601 ggcgtcgaag gcgtggatgg gctggcctgt ctccagcatg acgtagttgg tcgagtccac
     6661 gatggcgttt atgggccgca tgccggcggc gtggatcctg cgcctcatcc acagcggcgc
     6721 ctcctgcccg ggtcggagcc cggagatgcg cctgagatcg tatcgcggac acagatcctc
     6781 ggcctcgacc ctgagcccga aatcgtgggt cggcttcccg cctgcttcga aagttggttc
     6841 aggcactctg tagtccgtcc caaggatggc ggccagctcc ctggccaccc cgatcatgcc
     6901 ccaaagatcc ggcctgttgg gggtaacgtc gatgtccaaa acggtctcgc cgacggggaa
     6961 gtaatcggcg acaggacggc caacctcgta ggaatcgtcg agcagcagga tcccaccatg
     7021 atcggccgag attccgagct cgcgctcgct catcatcatc ccgtaagacc cgaggccgcg
     7081 cagcttcgcc tccctgagct tcgtcccgtc ggacataacg ctgcctggaa gcacgacgga
     7141 cacccgggcg ccggggtaag ggttcggtgc gccggccacg atctgcacct ccctgccccc
     7201 caggtcgacc ttggcgacaa agagcctgtc ggcgttggga tgcggcccga actcgatcac
     7261 ctcgcccacc actacctcgc cgtccatcac accgaggcgg tagacgccgt cgatctcctg
     7321 gcttcggagg gagaagagat ctacgagatc ttcggcggag aggtcgaaat cgacgtactc
     7381 gtgtagccag cttagcggaa cgcgcaagtt gaaccccttc tagaactgac gcagaaagcg
     7441 taggtcgccc tcgaagaaca ggcgaagatc cgggaccccg tacttgacca tcgccatcct
     7501 gtcggggccc atcccgaaag cgtagccggt gtactcttcg gggtcgtagc cgacctcttc
     7561 gagcacgcct gggtcaacca tcccggcccc tagcatctcc agccaaccag cgcctttgca
     7621 gaccttgcag ttcgagtccc tgccgccaca ggcaaagcag ctcatgtcca tttccacgct
     7681 cggctcggtg aactggaagt agctcggacg aagcctgatc tcgacgtcgt ccccgaagac
     7741 gtgacgggcc atcgcggcga gcgtcccctt caggtggcca agcgtgaggc ccctgtccac
     7801 ggctagacct tcaacctggt gaaacatagg cgtatgcgtc ggatcagagt cgcgcctgta
     7861 agtgcgcccc ggacacacta catagacagg cggctcctgc gccagcatcg tcctgatctg
     7921 catcggcgac gtctgcgtac gcaaaaccag cccgtcatcc acgaagaacg tgtcctgcat
     7981 ggtacgtgca ggatgccccg gcggtatccc cagggcggtg aagttgtagt aatcggtctc
     8041 cacctcgggt ccttcggcca cacgatagcc caggccgacg aagaagtcga cgacgtcgtc
     8101 gatcactatc tgggatggat gcaggtgacc cctctggaac gggacaccgg gcagcgtgac
     8161 gtcgacggct tcggcccgca gacgctcttc cctttcgcga gccgcgacct cttgggtcct
     8221 ggagccgaga gccgcctcga tctcgagcga ggcgagattg gaggacctgc caacctcctt
     8281 acgctcctcg ggcaggaggc tacctatgct cttcttgatc tccgtaagct ccgccgagcg
     8341 tccgagaaat cgtacgcgga cgtcttcgag atcggccgtg gaagcactgg aggagacggc
     8401 cgagagcgcc tcggtcttca attgctcgat acgctcgctc accgtcatag tgggcgatta
     8461 tggtagcaca cgcgaatacg cctcgtagag caggatcgac gccgccacag acgcgttcaa
     8521 cgagggcgcc agcgacggta taccaacctc catctcgcag gcccctacga tttcttcggg
     8581 cagcccggaa ccctcggcgc cgatacagat cacgagcctg ccacggggca gagactctgg
     8641 cgtgtccttt gcctccggaa ccgccgcgac gagaccgaaa ccggctgagc ccgcttcgtt
     8701 cagaaagccg agggactcta gctctcgcgc cacaggggtg tggaagatgg accccatcgt
     8761 cgccctgacc gtcttgggat tgtagaggtc tgcgcagccc ctggagaggg cgacgccggc
     8821 accgaaggcg tgggccgagc ggatggccgt gccgacgttc cccgggtcct gaattctatg
     8881 caacagcacg acccgccgca cagaggccag cagttcggtt gcggttgcgt ccagatatgg
     8941 aaagaccccg accggtcctg acggggtcgt gagcgccgaa acacgacgca actgctctgc
     9001 gctatcaaag acctgtattg gtgctgtggg agcctcttta agaatggcac ccccctcagc
     9061 aaggaacagg cgttcggcct cgcggtgttt cttggtcttg agccttgcag cccgtttcag
     9121 ggagatcgcc ggggtatccg gcatcccttg cttacgcgtc tcccttggcg gccttcgcga
     9181 cgtcgccgac ggtgatgcgc cccccctgtc ccgtaccctc cacgctggag aggtccacgt
     9241 tcagctcctc ggccttgcga ctggcggcat cggtagcctc gatctcatcc tccgaggttt
     9301 ttacctcttc cgtctcttcg acttgctctg caggtgcctc tggcgcgcgg acggtggatg
     9361 agacgggctg ttccggctcg gtgctccgct caacctccac ccgccgggcg accggttttc
     9421 cgtccagggc gttcttcgcc tgggccacga tggcgccgaa gatctcgggc tccgtggctg
     9481 ccaggtcggc caggaccttg cggtcgagat ccacctcggc cagccttaga ccgtggatga
     9541 gctttgagta agacaggtcg tgctcgcggg cgccggcgtt gatgcgctgg atccagagcg
     9601 ccctgaagtc cctcttgcgt tgcttgcggc cctcgtaggc gtagacgccg ctcttccaga
     9661 cctgctcctt ggcccgcttg tacgaggtgt gcttggtccc tcggtagccc ttcgcctgtt
     9721 cgagaacctt gcgacgcttc ttgcgggcgt ggacgctgcg tgcggtgcgt gccatgctac
     9781 ctctccccca gaagccgctt gagcttcttc tcgtccgagc gggtaaggtc ggtctccgtg
     9841 ttgagccgcc gcttctgctt cctgctcttc ttctccagca tgtggttgtg gccggcacgc
     9901 cagcgcttga gcctgccttt cttgcgcacc tcgaagcgcc cggcagcacc cttgtgggtc
     9961 ttcatcttcg gcatgtgtcc tccgcgtttc gtctgctatc tctacctgcg aagaggctgg
    10021 cggcacgcga gaaagagccg tttcgcctcc gtcattcgcc aggctagtgt atcaaatcgt
    10081 tgcttatctg cgagccgccc tctccctgcg tccgctggtc tgggaggcct ccgcctggga
    10141 cgattccttc ttcggggcca ttaccatcac catgttgcgc ccgtcgaggt tgggctggct
    10201 ctctatgcgt cccaggccgt cgaggtcgct ggcgagcctc ctcaggagcc tctcaccgag
    10261 ctcgggatgc tgcacctcgc gtccgcggaa catgatcgta accttcacct tgtcgccgcc
    10321 gcgaagaaag cgctcgacgt gaccgcgctt ggtattgaaa tcgtggtcgc ctatcttggg
    10381 cctgagcttt atctcccgca cgttgacgtt gacctgcttt ttgcgggccg ctttgcgggc
    10441 ctgctctttc tggtatttga acttgccgta gtccatcatg cggaccacgg ggggattggc
    10501 gttcggggct acctccacga ggtcgaggtc ggccctctca ccgtagtcca tggcctcgcg
    10561 gatgtgcttg atgccaagct gttcgccgtc tgccgagatc aaacgcacct cgcgcgcccg
    10621 gatctgaccg tttattcggg gctcttcttc agtcgctact gcgcaccact cctctcgaat
    10681 acgagctttc ggggccgtta gtccaccacg gacgatagat ttgctcgtgc gccggacgga
    10741 cgcggacccc aactacatga ttgattatac cgcggtgcct gacctcgtgt ctatatgagc
    10801 tgtcagcggt cagccttcag ctgtcagctc tttgtgcttg ctgtcggctg gtagctgtcg
    10861 gcgcagttcg cagagggtca ttgaccctct gcgaatcgcc ctaggtgcgc gagcggacct
    10921 ccccgaggac cgcctctgca aagccatcca gcgccatctc ctcctgttgc ttctccccgc
    10981 gcttgcgcac gttgacgcta ccctgttcgg cctccctgtc tcctactatg aggaggtagg
    11041 ggatcttctg cctggagttg tccctgattt tcttttgcat gctgttctgc gagtcgtcca
    11101 cctcgacccg gagatccctc tctgcgagct gcttcttgac ctctcgggcg tagcccaggt
    11161 gcctgtcggc gactgggatg actacggcct gcaccgggga gagccagacg gggaaggcgc
    11221 ccgcgtagtg ctcgatgagg acggccatga acctctctgt ggaaccggtg acggccctgt
    11281 gcaagagcac gggcgtgtgc gccttaccgt cttcgccgat gtactcgcaa cccagcctgg
    11341 ccggctggat gaagtcgacc tggatcgtgg agagctgcca ctcgcgaccc agcacgtctt
    11401 tggctacaaa gtcggccttc ggcccgtaga atgccgcctc tccggcctcc tcgtcgtagg
    11461 cgatggcggc ggtatcgagc gcctcgcgca actccttctc ggcccgcgac cacttggcgt
    11521 cgtcagccac gtacttggca acgtcttggt cgcgcaaaga gagctgcaca cggtattcgt
    11581 caaaaccgta agtgtcgagg acctcgcgta cgatctccag cgccctggcg aactcctcct
    11641 ggatctgatc ctccgtgcag aatacgtgcg catcgtcctg ggtcaggcta cgcacccggg
    11701 tgagtcccgt gagctccccg ctcttctcgt agcggtacag cgtcgcgaac tccgcatacc
    11761 tgagcgggag atctcggtag gagtgagccc gggagttgta gagcgtcatg tggctcgggc
    11821 aattcatggg cttgagcctg taacgcgtct caccgtcgat catcgggggg aacatcgact
    11881 ccctgtagtg ctccagatgt cccgacttcg cgaacagtcc ctcgttgacc aggtgccccg
    11941 tccacacgtg ctcgtagccg tggcgcgtct ggacctcgcg cacgtagtct tccatgaggt
    12001 gccgcatcat ctcccctttc ggcaagaaga gcgggatgcc ggctccggta tcgggcgaga
    12061 aagcgaacag ttcgaggtcc ttgccgatct tgcggtggtc cctggctctt gcctcctcca
    12121 gccgcctgag gtaagccttg agctccttct ccgtcggcca cgccgtaccg tagacgcggg
    12181 tgagcatggg attcttctcg tcgccgcgcc agtaggcccc ggctatgttc tgcagcttga
    12241 aggcgccgag cctgcccgtg ctagggacgt gaggaccgct acaaaggtcg aagaaatccc
    12301 cctgcttgta gaccgagatc tcaccgtctt ccaggccctc gatgatctcc gtcttgtagg
    12361 ggttgtccac gtacaggtcc tcggcctcgg ccttggagac ctcctcgcgc tggatcggaa
    12421 ggtcccgcgc gacgatctca cgcatccgct cctcgatgcg cggcagatcc tcctccgtga
    12481 gacgcccggc cacctcgata tcgtagaaga agccattctc tatcggcgga cctatggtca
    12541 gcttgctccc cgggtagagc tctacgatag cctgcgccat cgcgtgcgcg gtcgaatgcc
    12601 tgagcacttc gaggccctcg tatgaatcga tcgtaacgac ctcgaaatct cccccaccgt
    12661 tcagcggtgc atcgaggtcg atgagctgcc catccagctt cgcgaccacc gcatcgcggg
    12721 cgaggcgttt acctatcttc tcgacgactt cccgggcctt ctcacccggc tctatctcca
    12781 gttgtctgcc gtcgggtagc ttgacggagg ccatttttgt gcacctttct cagcaagggt
    12841 gggtaaggga aaccgtaaga ataactagat agttcgacgc acggcaccgg cgaggatcgc
    12901 gcagcgtggt gggtgatagt gatttcgaac caccgacctc ttccgcgcca gagatgtgta
    12961 gtgcgtttcc atacttgttc taagtttaca caatcctggc tgattgcgag aggcctatct
    13021 ctggaactac tttccatccc accactaacg accggttgcc cacgtaaccg cctagaatag
    13081 gatgcatcgg cactacctgg tggagtagag ctctcttgtg actttaccgg ggctcgtaga
    13141 atcggcccga tgctggggcc aaatagcagc gtttcggacc tatgtcagtc ttctcccgga
    13201 ctctttgaac ggccggggta ttgcgccccg gccccaggat aatctcggct gaaatgcgaa
    13261 cctgagaggt cctcgaggac cttacgggac ctgcggagcc ctcctgccta cttcacctct
    13321 tcctttcaca gtccacaacc cggtctttgg agtcgacgta ggccgtgtct gtacccctgc
    13381 cgcaattgac caggtccctg aagccaccct cactatagat aatgtcgttg ccccgtccgc
    13441 cgtattgctg atcgttgtcg tcaccaccgc gaatcttgtc ggggccggca tagccaagca
    13501 gcacgtcgtc gccactcaga ccgttgatgt ggtcattgaa cttggtgcct acgatcttct
    13561 cgccattctc ggtgccgaca taggttttgg ccagggctac cccgctgacc acgaccagcg
    13621 ccacggtcat cgccgcaagc aataatgccg ttctcctcac cgcatttcct ccttcctgtg
    13681 gccagggctc atctattccg tagccaccac tcgtttgtag atccagaatg caccttcgtc
    13741 ctcaacaatc gtcaagagag gataatggta cgccctgaat cccggctttc tcagactcgg
    13801 cgcgcatctt accaaatgcg gggcgcacag tgcataggcc gatcggcaca tcttgcacac
    13861 cgacatatca gcgagcaacc cctcttcccg aatgcgcttc cctacgaggc gtgggaacgg
    13921 gaagaccgga ggctttcgtt ttcgatcgat ctgcaggatc aaccttacac cccgatccgg
    13981 taatgagagg ccatcgagtg agctgggcgc cttcgcttca tcgttttagc tccctatagg
    14041 ccgcattgat ccatttcatc ctctcctcat cgcctgccct atctggatga tgaacccttg
    14101 cgagccttcg gtaagccgcg ctgatctcct tctgtgaggc acccagtcct actcctagag
    14161 cactgtgcgc ccatagggga ccggcggact ccgcttcgtg tttgggaggt tcgtcttctg
    14221 gctcgggtgg tgcctcttcc ggctccgcat tgggcagccg cacaccgtag cgctcggaga
    14281 acgcggcgaa ggggtctgcc gattcgtgtt tcttgaagaa cctcatctca ccctatgata
    14341 tccctacgtc catgatgatc catgatctag gcctctgact ccaggcgttc ccgggcggaa
    14401 agctccggga cacgggccat tccgcggcca tctccgccgc gttgttcgga cggatgcacc
    14461 gcgtgcgggc gacatagaac ccagggtggc tggcagcgca tcggttattg cggaggcgcc
    14521 cccttctcag ccgggtatca tccgcccgaa tatcttccct tcgaaggcga actcctcttc
    14581 tctgagcgca cgagtaaagt tgggcaccac ctctttggca ggcttccccc gtgcatcgga
    14641 tggatccatt gagggtcgag cttctcgagg cggtccacga cccttagcac tggttcctca
    14701 gcagcgaaga tgccggactc gcgatagccc tggcacatct ccccgcccag atcctcccgc
    14761 acgatcggcg gttgatcgcc ccattgcata tagaggtctg cgggaaagag gctgcccgac
    14821 gtctcctcga agaccatcat cgagtcccag tggtgaacgt ggggggtttc gataaagcgc
    14881 agcctatgct ccccgaggtc tacgctctcg ccatccttga agccctgcac ggggccggtg
    14941 tagtcccact gcgtcaggtt gagcaacgct cctgcatcgc tgcaggcca
//
LOCUS       DATMVF010000117         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_11970, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000117 DATMVF010000000
VERSION     DATMVF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11970"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VN178_05790"
     CDS             <1..311
                     /locus_tag="VN178_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80518.1"
                     /translation="TLGEGDLVSGFRVISTPGHSPGHVSLLRDADGLLFTADAFGCLP
                     RKLRVGVITALCTDPPMAKRSAQKLLREDFATVILAHGPVMRTDGREKLQRAIDRCRY
                     "
     gene            683..2041
                     /locus_tag="VN178_05795"
     CDS             683..2041
                     /locus_tag="VN178_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWS80519.1"
                     /translation="MLQRIVARLGHDTVEPGADTERTMGFGNTIMVWLAANMVVTTLL
                     TGTLFVPGILYLQAILIILLGTLLGVGVLSLVGCIGTRTGLPTMVLTRASFGIRGGTF
                     PALLNLAVLMGWSWVQALLAGIAVNSAVDSLTGFSNVVLFTVLCQSLVVLLALFGHQG
                     IERVEPFIAVVMIGLALFVFYTAFSAFGVGEFASIPADPSTGMTAAIAFDVVVATAIS
                     WTVLSADFNRNATSQLGGIGGTVIGYTASTSLAMTLGVTAAAYAQLKGGAAQGFDAAV
                     LVENFGIPAAIVVFLSVMATNTLVVYGMIMSYLNLRPQSGFLGPALVIGLISILGATW
                     QGVLNYFLDFLLLIGTFFVPVFAIMLADYYILRRGEYSVRDVLERRGGRYWFTGGFNL
                     PAWAAYVVGAGLALYWTRISPLSFGATVPVFALTFVLYLILRFAADRTKGRAAETDRV
                     PG"
     gene            2046..>2361
                     /locus_tag="VN178_05800"
     CDS             2046..>2361
                     /locus_tag="VN178_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWS80520.1"
                     /translation="MLQRIGRIVDLSRRMDAQTQVYPGDPHVRLEPATTLAADGVNVL
                     AVHLGSHSGTHADAPYHFVEGGARIDQLDPRLIVGPAVVMDVRGKGARERITVEDLRP
                     YEG"
BASE COUNT          374 a          741 c          783 g          463 t
ORIGIN      
        1 ggacgttggg tgagggcgac ctggtatccg ggttccgtgt gatctccacg ccaggccaca
       61 gtccaggcca tgtctcgctg ctacgcgatg cggacggcct gctgttcacg gccgatgcct
      121 tcgggtgtct gccgcgcaag ctaagggtcg gtgtgatcac agcgctctgc accgacccgc
      181 cgatggcgaa gcgctcggca cagaagctcc tgcgagagga cttcgcgacg gtgatattgg
      241 cgcacggccc ggtcatgagg acggatggga gagagaagct acagagagcg atagacagat
      301 gccgctatta gcgcgaacgc ggacatcata atgccatcgt cggctcggtc cacaacggcc
      361 tggaacctct atctctgtgg cacggtagcg tggcccgacg aggtcgtctt cggcgcgtcc
      421 ggtcataact gcgcattcta ggtgctcggg gaccatgcca gagccttaac gacttcggga
      481 cgataatctc cggcatacaa aggatgctac gcaggatctc ccaggaagat tgggtggacc
      541 gggtcgagtg gatgcgaatg gcgtagagca gttggcaagc tctccggcgc cgatcgcgtt
      601 gcccgcagct tacaacattg actcggggtg gcgggaaagg gtataagttc cgttaccggc
      661 aggggaacgg aagggagggc gcatgctgca gcggatcgtt gcgcgcctgg gtcacgatac
      721 tgtggagccc ggtgcggaca ccgagcggac gatgggtttc gggaacacca tcatggtctg
      781 gctcgcggcg aacatggtgg tcacgaccct gctcacgggg accctgttcg tgcccggcat
      841 cctgtatttg caggcgatcc tgatcatact tctcgggacc ctgctcgggg ttggcgtgct
      901 gtcgctcgtg gggtgcatag gcacgcgcac ggggttgccg acgatggtcc tgacacgggc
      961 ctctttcggc atcaggggag gaaccttccc ggcgctcttg aaccttgccg tcctcatggg
     1021 gtggagctgg gtgcaggcgc tcctggccgg catagccgtg aactccgcgg tcgactcgct
     1081 cacgggcttc tccaacgtcg tcctgttcac cgtcttgtgc cagagtctcg tggtcttgct
     1141 ggcgctcttc gggcatcagg gcatagagcg ggtcgagccg tttatcgcgg tcgtaatgat
     1201 cgggctcgcc ctcttcgtgt tctacacggc cttcagcgcc ttcggggtcg gcgagttcgc
     1261 ttccatcccc gccgacccct cgaccggtat gacggcggcc atagcgttcg acgtggtggt
     1321 ggcgacggcc atctcctgga cggtcctctc cgcggacttc aaccgcaacg ccacgtcgca
     1381 actcgggggc atcggcggga cggtgatcgg ctacaccgcc tcgacttcac tggcgatgac
     1441 tttgggcgtg accgccgccg cctacgccca gctgaagggc ggggccgcgc aggggttcga
     1501 cgcggcggtc ctggtggaga acttcgggat accggccgcc atcgtggtct tcctctccgt
     1561 catggctacg aacacgctgg tggtgtacgg catgatcatg tcctacctca acctcaggcc
     1621 ccagagcggc tttctggggc ccgcgctcgt tatcggcctg atctccatac tcggcgccac
     1681 ctggcagggc gtcctgaatt actttctgga cttcttgctg ctgatcggta cgttcttcgt
     1741 gccggtcttc gccataatgc tcgcggatta ctacatcctg cgccgcggcg agtactcggt
     1801 tcgggacgtc ctagagaggc gcggcggccg ttactggttc acgggcgggt tcaatctgcc
     1861 tgcgtgggcg gcgtacgttg taggagcggg gctcgcgttg tactggacca ggatcagccc
     1921 cctctccttc ggtgcgaccg tccccgtctt tgctctgact ttcgtcctct acctgatcct
     1981 ccgcttcgcc gcagaccgca cgaaggggag ggccgccgaa accgaccggg tgccgggtta
     2041 ggcccgtgct ccagaggatc ggccgcatcg tcgacctctc ccgccggatg gacgcacaga
     2101 cccaggtcta tcccggcgac cctcacgttc gcctggagcc cgcgacgacc ctggcggcgg
     2161 atggagtcaa cgtccttgcc gtccacctgg ggagccactc cggcacccac gccgacgccc
     2221 cctaccactt cgtcgagggc ggcgcgcgca tagaccagct agacccgcga ctgatcgtcg
     2281 gccccgctgt agtaatggac gtgcggggca agggggcgag ggagaggatc acggtggagg
     2341 acctaaggcc ctacgagggc c
//
LOCUS       DATMVF010000118        22628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_386756, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000118 DATMVF010000000
VERSION     DATMVF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22628
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386756"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1956
                     /locus_tag="VN178_05805"
     CDS             169..1956
                     /locus_tag="VN178_05805"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HWS80521.1"
                     /translation="MTSNNGRPERRFRSRDWFDDPHRRDQSALYLERFMNYGITPDEL
                     RSGRPVVGIAQSGSDLSPCNRHHLELAGRVRDGIRDGGGIPMEFPVHPINENCRRPTA
                     ALDRNLSYLGLVEILYGYFIDAVVLTTGCDKTTPAAIMAASTVDIPAIVLSGGPMLDG
                     YFEGELVGSGTAIWKSRRRLAAGQIDEDQFFETALASAPSAGHCNTMGTASTMNALAE
                     VLGLSLPGCAAIPAPYRERGQMAYDTGRRIAEMAYEDLRPSKILTRESFLNAIVATTA
                     IGGSTNAQPHIMAMARHAGVEIKPSDWEHGYDVPLLVNMQPAGEYLGERFHRAGGVPA
                     VLGELLQAGKIDGGTRSVTGRSLAESVAGCESKDREMIAAHDEPLQEKAGFRVLSGNL
                     FDFAIIKTCVISDEFRERYLGDPANKDVFQGRAVVFDGSDDYHERINDPDLDIDETSI
                     LVIRGAGPVGWPGSAEVVNMQPPDSLLKRGINTLPTIGDGRQSGTSDSPSILNAAPES
                     AAGGGLAWVRTGDTIRIDLGTGRCDMLVSDGEIERRKREEGIPDYPESQTPWQEIYRA
                     SVDQLDGGGVLESALKYRGIATKTPRHNH"
     gene            1979..2128
                     /locus_tag="VN178_05810"
     CDS             1979..2128
                     /locus_tag="VN178_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80522.1"
                     /translation="MQRAGFIGLGAMGAAHRLYEEGSSLGGEDDSGVVRVFETTNEAG
                     DSLRI"
     gene            2218..2574
                     /locus_tag="VN178_05815"
     CDS             2218..2574
                     /locus_tag="VN178_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWS80523.1"
                     /translation="MERVMLVEDHSAFAQAFELVLGQVEGTEVILARTLEEGRALLRG
                     GGSFDVVLLDLTLPDGEGTELMAEIRRRHPEAPIAVLSARDDVDEAASNAGADVAIHK
                     NTPLPDIISSLKQLAG"
     gene            2827..3939
                     /locus_tag="VN178_05820"
     CDS             2827..3939
                     /locus_tag="VN178_05820"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWS80524.1"
                     /translation="MLLCALLAFVAAVLAFSANSARGAATLPPNFARSQVVGGLASPT
                     AMEFAPDGRLFVAEQRGTLRVVKAGGNLATFLDISGGVDSAGERGLLGIAFDPAFSSN
                     HYLYLYYTQKATGTTPAHNRVIRLRARGDRAVAGSMKLILRLDNLSSATNHNGGAIHF
                     GEDGKLYVAVGDNAKSDNAQTLSNLKGKMLRINKDGTIPPYNPFYKRATGRNRAIWAL
                     GLRNPFSFAIQPRTGKMLINDVGEQRWEEIDRGAAGANYGWPRYEGPESDPKYRNPVF
                     AYRQGSTKTTGCAITGGAFYNPATKRFPRGYVGDYFFADYCSGWIRKLDATTGDVSGF
                     ATGLGNPVALEVSKGGGLYYLDRGAGSVGKIRYTGT"
     gene            4097..4852
                     /locus_tag="VN178_05825"
     CDS             4097..4852
                     /locus_tag="VN178_05825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HWS80525.1"
                     /translation="MAFSEEVAWVTGSSTGIGRAVAVVLAGQGRRVVVHYNTSEDEAR
                     EVVEKIEASGGEASIVGGDVSDAAEVGRMVGEIEDRFGRLDVLVNNAGSLIERRSLSE
                     MTDDLWDRVMDVNLKSVYLVSRAVLPIMRRQGRGRIINMTSIAARNGGGPGSIAYATA
                     KGGVSTLTRAMAKELVSENILVNGVAPGVITTPFHDRFTPPDVRENLTNAIPMGREGT
                     PEETAGVVAFLASPAADYLVGEIIEVNGGQLMS"
     gene            complement(4900..5850)
                     /locus_tag="VN178_05830"
     CDS             complement(4900..5850)
                     /locus_tag="VN178_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS80526.1"
                     /translation="MENSAVDPSSRRALGGTGLSVSPLCVGCAPLGDMPETFDYSVDE
                     ERASETLRAVLESQINFLDTAASYGDGESERRIGEVFEETGGPPKDVVLATKADRDLR
                     TGDFSGDQMRRSVERSLRLLGMESLQLVYLHDPEHESFDHMMSDGGPVEVLTGLKEEG
                     VIQHLGVAGGPIDLMIRFVETDLFEAVITHNRYTLVNRTADPLFDVAAERGVAALNAA
                     PYGSGILAKGPDAYARYEYSEAPKEMVEQVRAMQDACREFDVPLAAAALQFSLRDPRI
                     VSTIVGMSRPERVRQTLDLATHPIPAELWEHIDALEAPNG"
     gene            complement(5870..7027)
                     /locus_tag="VN178_05835"
     CDS             complement(5870..7027)
                     /locus_tag="VN178_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS80527.1"
                     /translation="MSKSLKALLVGGPMYDPLYTRLAEFEEREGLRVETVVAPTHPDL
                     NEQIEAEFASGGASYDLISTHTKYAPSQRQWLTSLDDDLDDGELEPFTPRTLELARMD
                     GSLYGVPRNLDVKLLLYRTDYMEDKPSSWEDLLEIATRLRSDNLYGFAFPGKESGLFG
                     HFFELHAMAGGRMFEDEGPPVPRLDDEAGRWALGLLKDLYERAAPPETPDWHYDEVAA
                     CFRGGRAVMSTDWPGGFYTYLDPDQSAVVDRFDVALYPEGPAGRHIYSSSHTFAIPAT
                     VSDRPAAVELLRFLTSRESQAYEAGLGTLPARSDSLQDVRSGAGPFAERRWNLLERAQ
                     EAALIPPKHANYPAVEDAIWQGIREALLGNKSVEQALEDTEAAARRAAEGL"
     gene            complement(7024..7653)
                     /locus_tag="VN178_05840"
     CDS             complement(7024..7653)
                     /locus_tag="VN178_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWS80528.1"
                     /translation="MRTILCYGDSNTWGSDPETRERFPEDVRWPGVLRKRLGEEYRVI
                     EEGLPGRTTVRDDPIEGDHKNGRTYLMACLESHRPLDLVTLMLGTNDLKARFGSSASD
                     IAQGAASLADMMLRSGCGPDGGAPIVLLISPPVVATLTDMAQMFEGAEEKSRQFSGHY
                     ERFAEQHGCEFFDASQVIVSSDVDGIHLDAPEHRKLGEAVAARLGVIFR"
     gene            7902..9269
                     /locus_tag="VN178_05845"
     CDS             7902..9269
                     /locus_tag="VN178_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWS80529.1"
                     /translation="MVTGHRVAGEREEPLSAVLVERAMNRRDFLRMSGACLAGVGLLG
                     VGACGGGGTSGTLRWSMWSDTPEETRVWKDLARDVHEKYPDITVKLETVTFADYWDKL
                     TTQIASENEADIVAMQSLRMPGYAARGALQPLMPFIEDDSDFDVDDFFGPIRQGLSVN
                     DELYALGYDIGPIILYYNKDLFGKAKVEPPPAETPMSWEEFREKAEQLTNPGAREYGF
                     VEQPVFDFMVPWLWSGGGGYMNAAQTECTLDTPESIGAMEFVIGMITDKIAAPVTDLA
                     NPSFAVEEFFGGKIGMHVDGPWQFINIRENSDFEWDVAPMPAGPAGSVTWAAGSGFGI
                     SNTTENADAAWTALKTITSTESLEKLVKAGRGYPARESAVSTFEKPPPQNVDVVQKIL
                     VSDIGETRPFVTTTTWQETTVMLTRDFSPVFLGKQSVDAVVSKVKPQFDKLLKEHQEI
                     LEQQS"
     gene            9284..10207
                     /locus_tag="VN178_05850"
     CDS             9284..10207
                     /locus_tag="VN178_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS80530.1"
                     /translation="MAGTAQTGRGPEKVPWKNREHEGLAAGLFLLPNLIGFLIFTAIP
                     VGAAFVLAFYDYDLLLGASWAGLENFRELFTVDRVFRAALFNTVYFTAVSVPLSVVLG
                     LATAILVNQALRGVVIFRSIFLLPYVTVTVALSLVWKWLYLPDIGLINSVLGAVGIDG
                     PAWLTSQTWAMPALILMSVWKGFGYNMVIFLAGLQGIPDHLYDAAKVDGATAWRRFLN
                     VTLPLLSPTTFFVVVISVISSFQVFDQALIMTNGGPGTATTTLVLYIYQKGFQSFDMG
                     YAAAVALVLFAVIFVFTVVQFLFQRRWVTYE"
     gene            10269..11102
                     /locus_tag="VN178_05855"
     CDS             10269..11102
                     /locus_tag="VN178_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS80531.1"
                     /translation="MSPGGKPYWPILIYATLMAMAFLTLVPIIWMVLTAFKSAPEVAA
                     NPPTWWPREWHPENFAAAWNFAPFGRYLINSLVMVGGITILQTVTSALAAYAFARLRF
                     RGRDLLFLLYLGTLMIPPQVTVIPQFILIKELDWVDTYQGLIVPQAFTAFGVFLLRQF
                     FLTIPYELEDAARIDGASRFGCFWRIILPLSGPALATLAVFAFLFHWNNLLWPLIVSN
                     SDATIPVVVGLRNFQGQYGTEWNLLMAAAAIATIPTVIVYLFAQRWFVRGITMSGFGG
                     R"
     gene            11131..12420
                     /locus_tag="VN178_05860"
     CDS             11131..12420
                     /locus_tag="VN178_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HWS80532.1"
                     /translation="MKQELGRTTAADTEWFTRDRFGLFIHWGIYALPARHEWVKQRER
                     IRDEGYQKYFDHFYPDLYDPEKWAREARNAGMRYFVVTTKHHDGFCLWDTDLTDYKAT
                     NTLWGEDLIAPMVEAFRAEGMKVGFYHSLIDWHHPEFPVDGLHSRRDDTEYIEANRDR
                     DISRYREYLHGQTRELLTRFGRVDVMWFDFSYSDRVWGGKGKEDWGSVELMEMVRGLQ
                     PGIIVNDRLEIGGDLKTPEQYQPRSWPEVEGERTLWEACQTLNGSWGYDRDNLDWKPT
                     DMLVRMLVDTVSKGGNLLLNVGPNARGELESRAVERLRGLGEWMRLHERSVRGCTASD
                     LTPPPDCRYTENGNRLYLHLFGWPFKHVHLEGLAGRVEYAQLLNDASEIKMKVIDPAQ
                     RAQNTTVGGIPEGTLTLELPVQRPPVEVPVVELFLKG"
     gene            12426..14462
                     /locus_tag="VN178_05865"
     CDS             12426..14462
                     /locus_tag="VN178_05865"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HWS80533.1"
                     /translation="MGRLRLRQVHLDFHTSAECEGVGADFDPEVFAQTVRMGRVDSMT
                     VFAKCHHGFSYYATEVGTRHPNLALPDLMGAQIEALHGIGVLAPIYVSIMWDDLAGEK
                     EPGWVIARRDGSLMIRPPLSNESPLAGGWGWTTLDVSTGYGDYLVAQTEEICDRYDVD
                     GFFFDICFPVPNYSPWGQAKMREAGVSLDDEQAVLRFAEWKLHGFLERLSEAVRAKVP
                     GATIFYNGTVTPRMAETLPYQTHFEIESLPTSSGLWGYLHYPVMARQARTYGVDFLGM
                     TGRFHKAWADFGGLKTSDQLDYECGTILAAGGEISVGDQLHPRGVLDPAVYRLLEHSF
                     GKVERLEPFLHGAKPTAEVAILSRYEPSALGSVPTNAHVAEVEGAAQLFLEAAIQFDI
                     IDPSHGSFLNYAAIVLPDGISLYEDLRAKLESYTSAGGKLILGGTAALDEETGQFGLE
                     SIPVAYEGRAPTVPSYLRLDEALAGESELATDYDYVFYDRAHLVRPLEGAKVRGELRS
                     ALFDRTWEHFTSHAQAPVGESLDSALVVESEGVLYFATPLFAAYRDHDYWVYREVALH
                     AIRDFLPRPKLKLSGPGWVEATLHEQPADGDHPERRIVHLVAYHPRRTTQPVQHVDQS
                     WPTSGLGIEILTDGSTVGRAYLAPENEPLEVEVVDGYARIALPPVGTHAVVVLE"
     gene            14467..15192
                     /locus_tag="VN178_05870"
     CDS             14467..15192
                     /locus_tag="VN178_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWS80534.1"
                     /translation="MALTDDAITSIKQMILSGQVGPGEKLPREKELATRLGLSRSSLR
                     EAIRALILAGILKTRQGDGTYVTSLEPHQLLETMSLAVDFMQNHTLVELFEVRRMIEP
                     AATALATSRITPEALERLEETLRRMDSAVSVEELVEADDDFHRTIASATGNAALAALL
                     QNLYGRTLRARIWRGLAEEGALDRTRISHRSIYGAISNRDAELARSAATMHIAEVEAW
                     FRSLPKFTPSNDERTGTRETPSR"
     gene            15331..16989
                     /locus_tag="VN178_05875"
     CDS             15331..16989
                     /locus_tag="VN178_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HWS80535.1"
                     /translation="MAQRQERTGIDSTVFWIAAIISAVFVLWGILFTDNLAAVFDAVL
                     WSFLVPNFGWVFILSSFGFLAFSLYLAFSRYGKIRLGGQDEEPEFSTVSWVAMMFSAG
                     MGIGLMFFGVAEPLSHMSSPPGGTAEAGTRGAAQVAMEYSYFHWAFHPWAIYAVMGLA
                     LAYFVFRKGMPNLISTAFYPLLGDRVYGPLGKTIDILAIFATLFGSATSLGLGALQIN
                     QGLNAMFGFGGREAVGLAIVVIAILTAGFILSAISGVHKGIQWIANTNMVLAVFLLVF
                     VFLLGPTVFILNTFVESLGGYLANLIPMSFRTAAFSDGAFVSGWTVFYWAWWISWAPF
                     VGTFIARISRGRTIREFVFGVILAPSVVSFIWFAILGGTAINLQLTGAADIAETAANN
                     QPAALFLLLEQFPLFWLMAGITIILVALFFVSGADAASMVMGMLSSKGNLHPQSWNMV
                     VWGVFTGAAAAICLLSGAIQGSVDAALLSLQAVAIASAAPFVLILIGLCFSILKALRE
                     ERVPGVTPGLEAPEPGRAMSRQPGTTAPQQMATERPEEQRGPRD"
     gene            17092..17541
                     /locus_tag="VN178_05880"
     CDS             17092..17541
                     /locus_tag="VN178_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWS80536.1"
                     /translation="MGRTRNPFRGLMDHMSEMARMREYAEGGGQEDQRRTHATAWIPT
                     ADIFAKGEDLVIRCELAGVEQGDVEVSLSGGVLTIDGERKGEPEDVSYYARERYYGHF
                     RRTMNLPEGINSNMISADFQNGLLEVTVQGGADHPEPERIQIESRTS"
     gene            17889..20330
                     /locus_tag="VN178_05885"
     CDS             17889..20330
                     /locus_tag="VN178_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS80537.1"
                     /translation="MESSAGCVIIGAGIVGCSVAEHLSRLGWRDVVVLDQGPLFEAGG
                     STSHAPGLMFQTNPSKTMTDLARYSVGRYMELELDGEPCGYQVGGIEVAETQERWRDL
                     QRKHGLATSWGLESGLISPQECAELVPVLDSNTIRGGFYVPTDGIAKAVRACEAMAGL
                     AGERGLKFYGETEVTGIEVKDGRVRSVETDRGRVETELVVSCAGMWGPRIGRMVGMRV
                     PLVPMQHQYAWTTPLEELAGEAREVVHPILRHQDFSMYFRHRGNHYGIGTYKHRPMPV
                     SADEIRRDYRGEGEFPSVWEFTPEDFESSWQEARKMLPALERVEIDRPMNGLFSFTPD
                     GGPLLGESKEAKGFWIAEAVWITHAIGVGKVMAEWITEGVPSMDISGADVHRFEPYAL
                     SPSYILARSAQSFREVYDIIHPQQPMEEPRPLRASPFYPRQQALGAYFLEASGWERPQ
                     WFEANESLLDGHEVSDRGEWAGRYWSKVVAAEALATRERVALYDMTSLKKAEVAGPGA
                     LDFLQGLTTGQLDKPAGKVTYTLMLDSDAGIRSDVTVARLGEDHFQLGLNGPRDIEWM
                     ERHLPQDGSVHVRDITPGTCCIGVWGPAARDVVQSLSEDDFSNEAFGFFNARRAYLRE
                     VPVTALRLSYVGELGWEIYTSADLGLRLWDLLWEAGRPYGILASGRGAFNALRVEKGY
                     RAWGTDMTTEHDPYEAGLGFAVKEDKGDFVGREALLERKEAGPRRRLVCLVLDDPSVV
                     VMGSEPVWADTAVGYVTSADFGYSLGQSIAYAWVPPEHAGVGTRLEIEYFGVRHRATV
                     TEEPLFDPAMKRMRA"
     gene            20371..21576
                     /locus_tag="VN178_05890"
     CDS             20371..21576
                     /locus_tag="VN178_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWS80538.1"
                     /translation="MNDNVNPDPGILQYTRIFKSPYFYGSRRHGVQKYSVYNHHYHPR
                     YYRDPVEEYWQLLEGVTLWDVGGAERQVEITGPDAFEFTNTLTPRNLNKCAVGQCKYA
                     FITNAEGGIINDPVLLRLDENHFWLSLADSDVLLWAQGLAYNMGLNVEIKEPDVGPMQ
                     VQGPKSREVMTDLFGEEILEVPYYYMVHRELDGMELVISRTGYSGELGYELYLKNASR
                     DGLKLWDRVLQAGEPYGISPIGPCHIRRIEGGILALGCDMWYDTNPYEVGYGYKWMVE
                     LEQEQHFVGREALKKVSEEGVSRKLVGVELGGSNVGSYNDGSMIDYFPVHTHGERIGK
                     VTSACYSPRLEKNIGFAMVPVEYSEIGIELEVERPEETVSATVADRVFFKPEHAEQTL
                     DATGSSKGA"
     gene            21654..22481
                     /locus_tag="VN178_05895"
     CDS             21654..22481
                     /locus_tag="VN178_05895"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWS80539.1"
                     /translation="MAEFLRHPGYEVIPLRGTEEAVVEHVPKDIALTVTASPGKGTWA
                     TIELAERLAGHGFGVAPHLSARLIRDRSELGEILHRLRDAKIRNAFVVAGDIDEPAGE
                     FEGAAGLLRAMNEIGHDLDEVGITGYPESHPKISDEMTIQAMYDKAPYATYIISQICF
                     DPEVTSDWARRVWSRGVKLPIRIGMPGSVNRQKLVRISTSIGLGESARFLNRQRNWLL
                     KMFLPGGYSPDRLIEGLKPGFADPESNIQGLHIYTFNEVETTEAWRREMLERAETSA"
BASE COUNT         4314 a         7026 c         7259 g         4029 t
ORIGIN      
        1 gatgtggccc aggcatgcct cttcctcgcg acgctcccgc cccgcgccta cgtccccgag
       61 ctgacgatgg tcccgaccgc gatgcaggcc ctcggcaaga cctcgacggc gacgccaccg
      121 gtctcatggg atgatgagta ggacgaagac ccgacaagga gatccgccat gacttcaaat
      181 aacggccggc cggagcgcag gtttcgctcg cgcgactggt tcgatgatcc ccatcgcagg
      241 gaccagagcg cgctgtatct ggagcgcttt atgaactacg ggatcacacc ggacgagttg
      301 cgctccggca ggcccgtggt cggcatcgcc cagagcggaa gcgacctctc gccctgcaac
      361 aggcaccacc tggagctcgc cggtcgtgtg cgcgacggga tacgcgacgg tgggggcatc
      421 ccgatggagt ttcccgtgca ccccatcaac gagaactgcc gcaggccgac cgccgcgctg
      481 gaccgcaacc tctcctatct gggtctcgtc gagatcctct acggttactt tatcgacgcc
      541 gtggtgctca cgacgggctg cgacaagacg acgccggctg cgataatggc cgcctcgacc
      601 gtggacatcc ccgccatcgt gctctccggc ggtcctatgc tcgacggtta cttcgagggg
      661 gaactcgtag gttcgggcac ggccatctgg aagagccggc gccgtctggc cgcgggccag
      721 atcgacgagg atcagttctt cgagaccgcc ctggcctcag ccccgtctgc gggtcactgc
      781 aacaccatgg gcacggcttc gacgatgaac gccctggccg aggtgctagg tctctcgcta
      841 cccgggtgcg ctgccatccc cgcgccgtat cgcgagcgcg gccagatggc ctacgacaca
      901 gggaggcgca tcgccgagat ggcctacgag gacttgcgcc cttcgaagat cctgacgcgc
      961 gagtctttct tgaacgctat cgtcgcgacc accgcaatag gtggatctac caatgcccag
     1021 ccgcatatca tggccatggc gcgccacgcc ggggtagaga tcaaaccctc cgattgggaa
     1081 cacggctacg acgtgccctt gctcgtcaac atgcagccgg cgggcgagta cctgggagag
     1141 aggttccacc gcgctggcgg cgtgcctgcc gtgttgggcg aacttctgca ggccggcaag
     1201 atcgatggcg ggacgaggtc cgtcaccggc aggtcgctgg ccgagagcgt cgcaggatgc
     1261 gagagcaagg accgggagat gatcgcggcc cacgacgaac cgcttcagga gaaggcgggt
     1321 ttcagggtcc tctccggcaa cctcttcgac ttcgccatca tcaagacctg tgtgatctcg
     1381 gacgagttcc gcgagcgcta cctgggggac ccagccaaca aagacgtgtt ccagggccgc
     1441 gcggtcgtct tcgacggctc cgacgactac cacgagcgca tcaacgatcc cgacctcgac
     1501 atcgacgaga cctctatcct ggtgatccgg ggcgcaggac ccgtcgggtg gcccggctcg
     1561 gccgaggtag taaacatgca accccccgac tcgctcctca agcgtgggat caacaccctg
     1621 cccaccatcg gagacggcag gcagtccggc acctcggaca gcccctccat cctgaacgcc
     1681 gcgcctgaga gcgccgcagg cggcggcctg gcctgggtgc ggacggggga taccatccgc
     1741 atcgatctgg gtaccggacg ctgcgatatg ctggtctccg acggggagat agaacgccgc
     1801 aaacgggagg aaggcatacc cgactatccc gagagccaga cgccctggca ggagatctac
     1861 cgcgcctccg tggatcaact cgacggcggt ggggtgctcg agagcgccct gaagtacagg
     1921 ggcatcgcca caaagacacc acgccacaac cactgatggg gttctaaggg aggggtggat
     1981 gcaacgcgcc ggtttcatag gactcggggc gatgggcgcc gcccacaggc tatatgagga
     2041 aggatcctcg ctcggcgggg aggacgattc cggcgtcgtg cgtgtcttcg agacaacgaa
     2101 cgaagcagga gattctcttc ggatctagtt catttgtacg atttacgtcc tgcgctttct
     2161 ccgttagggt ggtgctcgga cggtttgtag ggcagaagac aacacggagg tgtagcgatg
     2221 gagagggtga tgctcgtaga agaccactcg gccttcgcgc aggccttcga actcgttctc
     2281 ggacaggtgg agggcacgga ggttatcctg gcccgcacac tggaggaagg cagggctctg
     2341 ctaaggggcg gcgggtcatt cgacgtggtg ctactcgacc tgacactccc cgacggggaa
     2401 gggacggagc tcatggccga gatcaggcgc cgccatccgg aggcgcccat cgccgtcctc
     2461 agcgccaggg acgacgtcga tgaggccgcc tcgaacgccg gggccgatgt cgcaatacac
     2521 aagaacacac ccctacccga cataatctct tccctcaaac agcttgcagg ataagggagc
     2581 cttttcgcgc tgtggctacc tgcaagcggg atgattgcta cgagagtatc gtcgccggta
     2641 tactgataaa cgtcccagat acaggcaacc agccgggtgg ctgagacgca aagctacagg
     2701 ccgctcccaa caggaggcgg ccgtgccgcc gaaaggataa tgctagtgag atcatcctgt
     2761 tctccctgta ctagatcgga gacggttcgc tctgataggg ggattccagg cgggctgctc
     2821 ttgacaatgt tgctctgtgc cctgttggcc ttcgtcgcgg ctgttctggc tttctccgcc
     2881 aactctgcgc ggggcgccgc aacgctcccg cccaacttcg cgcgctcgca ggtcgtcggt
     2941 ggcctggcga gtcccacggc catggagttc gccccagacg gtcggctgtt cgtggccgaa
     3001 caacgcggca cgctgcgggt ggtaaaggca ggcggaaatc tggcgacatt cctggatatc
     3061 tctggcgggg ttgactcggc gggggaacgc ggcctgctag gaatcgcttt cgaccctgct
     3121 ttctccagca accactacct ctatctctac tacacgcaga aggcgacagg caccaccccg
     3181 gcccacaacc gggtgatccg cctcagagcc cgcggtgata gggccgtcgc aggcagtatg
     3241 aagctcatcc tccggctgga caacctgagc agtgccacaa accacaacgg aggggccata
     3301 cacttcggcg aggacggcaa gctctacgta gccgtgggtg acaacgccaa gagcgacaac
     3361 gcgcagaccc ttagcaacct gaaagggaag atgctgcgca tcaacaagga cggcacgatt
     3421 cccccctaca accccttcta caagcgggct acagggagaa accgcgccat ctgggcgctg
     3481 gggcttcgca accccttcag ctttgccatc cagccccgca cgggaaagat gttgatcaac
     3541 gatgtcggtg aacagaggtg ggaggagatc gatcgggggg ctgcgggagc gaactacggg
     3601 tggcctcgat acgaaggccc cgagtcggac ccaaagtacc gcaatcctgt gtttgcctac
     3661 agacaaggaa gcacgaagac cacaggttgc gccataaccg gcggcgcctt ctacaatccg
     3721 gccaccaagc gattccctag aggttacgtc ggcgactact tttttgcgga ctactgcagc
     3781 gggtggataa ggaagctcga tgctactaca ggagacgtca gcgggttcgc caccgggcta
     3841 ggcaaccccg tcgccctcga ggtgagcaag ggcggtggcc tctactacct ggacaggggc
     3901 gcgggttcgg tcggaaagat ccgctacaca ggcacctagg cgctaaaccg ttcgtcaaag
     3961 ggaccgaacc ggcagcgtac cggctttcta atccacagcg aacctcccac gtcccgtgtt
     4021 gttttccccg cccggaacca gtatgttgtc tggggcatcg cttgcagaag aacccttaca
     4081 gggaggtaac ggtcggatgg ccttctcgga agaggtcgcg tgggtgacgg ggagcagcac
     4141 ggggatcgga cgggccgtag ccgtggtgct cgccgggcag ggacgccggg tggtcgtcca
     4201 ctacaacacg agcgaggatg aggcgcggga ggtggtcgag aagatcgaag cttcgggcgg
     4261 ggaggcttcg atcgtcgggg gcgacgtctc cgacgctgcc gaggtcgggc ggatggtcgg
     4321 ggagatagag gaccgtttcg ggcggctcga cgtactggtg aacaacgcgg ggtcgctgat
     4381 cgaacgcaga agcctatccg agatgaccga tgacctctgg gaccgtgtaa tggacgtaaa
     4441 tctgaagagc gtctatttgg tctcgcgggc ggtgttgccg atcatgaggc ggcaggggag
     4501 aggcaggatc atcaacatga cctcgatcgc tgcccgcaac ggcggcggtc ctggctccat
     4561 cgcctacgcc acggccaaag gcggcgtaag caccctgacc cgcgcgatgg ccaaggaact
     4621 cgtctccgag aacatcctcg taaacggggt cgcacctggc gtaattacca cccctttcca
     4681 cgaccgcttc acgccccccg acgtccgcga gaacttgacg aacgccattc cgatgggacg
     4741 agagggcacc cccgaggaga cggccggggt cgttgctttc ctcgcttctc cggccgcgga
     4801 ctacctggta ggcgagatca tcgaggtcaa cggcggccag ttgatgagct agccacagca
     4861 aggaacccga gcgcgacatg cgaagctgcg tagcgacctc taaccgttcg gagcctctag
     4921 cgcatctatg tgctcccaca gttcggccgg tatcggatgg gtggcgaggt ccagcgtttg
     4981 tctcaccctc tcggggcgac tcatgccgac gatggtcgag actatgcggg ggtctcttag
     5041 tgagaactgc aaggctgcgg ctgccagggg aacatcgaac tcgcggcagg catcctgcat
     5101 ggcgcgcacc tgttcgacca tctctttcgg cgcctcactg tactcgtagc gggcgtaggc
     5161 gtcggggccc ttggcgagga tgccgctgcc gtacggggcg gcgttgagcg ctgctactcc
     5221 gcgttctgcg gcgacgtcga agagagggtc tgcggtacgg tttacgagcg tgtagcggtt
     5281 gtgggtgatg acggcctcga agagatccgt ctcgacgaac cggatcatga ggtctatcgg
     5341 cccccccgcg acgccgaggt gttggatcac tccttcttcc ttgaggcccg tcagtacctc
     5401 gaccggacct ccgtcggaca tcatatgatc gaaactctcg tgctccgggt cgtgcaggta
     5461 gaccagctgg aggctctcca tgccaagcag ccgcagggac cgctcgacgg agcggcgcat
     5521 ctgatctccg gagaagtccc cggtcctgag atcccgatcc gccttggtgg caagcacgac
     5581 gtctttggga gggccgcccg tctcttcgaa cacctcgcct atccggcgtt cgctctcccc
     5641 gtcgccgtac gaagcggccg tgtcgaggaa gttgatctga ctctccaaaa cagccctcag
     5701 cgtctcgctg gcccgctctt cgtctaccga ataatcgaag gtctctggca tgtctccgag
     5761 cggcgcgcat ccgacgcaca acggcgagac cgagagtcct gtaccaccca gagccctcct
     5821 cgacgaaggg tcaaccgcac tgttctccac tattcctccc gtccgggcgc tacaagccct
     5881 ctgccgcccg acgcgccgct gcctctgtgt cctccagcgc ctgctccaca ctcttgttac
     5941 ccagaagcgc ttccctgata ccctgccaga tggcatcctc gaccgcagga tagttcgcgt
     6001 gcttgggtgg gatgagcgcg gcctcctgcg cccgctccag caggttccaa cgccgttcgg
     6061 cgaacgggcc ggctccggac cggacgtcct gcaaagagtc actccttgcg ggaagggtac
     6121 ccaggccggc ctcgtaagcc tgcgactcgc gcgaggtcag gaagcgaagg agctcgacgg
     6181 cggccggacg gtcgctgacg gtggcaggga tggcgaaggt gtgcgagctc gagtatatgt
     6241 gacgtccggc tggaccctct gggtagagcg ccacgtcgaa gcggtctact acggcgctct
     6301 ggtcgggatc caggtacgtg tagaaaccgc cgggccagtc ggtgctcatt acggctctgc
     6361 ctccacgaaa gcaagcggct acctcatcgt aatgccagtc cggggtctca gggggcgctg
     6421 ccctctcgta gaggtctttg agtagaccga gcgcccaacg gccagcctcg tcgtccaggc
     6481 gcggaactgg cggaccttca tcctcgaaca tcctgcctcc ggccatcgcg tgtagctcga
     6541 agaagtggcc gaagagtcct gactccttgc cggggaaggc gaagccgtag aggttgtcgg
     6601 acctcagccg cgtcgctatc tccagcaggt cctcccacga ggagggtttg tcctccatgt
     6661 agtcggtgcg gtacaggagg agcttcacgt ccaggttgcg cgggacgccg tagaggctcc
     6721 cgtccatccg agccagctct aacgtgcggg gagtgaaggg ttccagttcg ccatcgtcca
     6781 ggtcgtcgtc gagcgaggtt agccactgtc tctggctcgg ggcgtatttg gtgtgcgtcg
     6841 agatcaggtc gtaggaggcg ccgcctgatg caaactcggc ttcgatctgc tcgttgaggt
     6901 cggggtgtgt gggggcgact accgtctcga ccctcaggcc ctcgcgctcc tcgaactccg
     6961 caagacgcgt gtacagcgga tcgtacatcg gtcccccaac cagcagagcc ttcaaactct
     7021 tactcatctg aagatcactc ctaggcgggc tgcgacggcc tcgccgagct ttctgtgctc
     7081 gggggcgtcg aggtggatgc cgtccacgtc gctggagacg atgacctggg aggcgtcgaa
     7141 gaactcgcac ccgtgctgct cggcgaagcg ctcgtagtgc ccgctgaatt gcctggattt
     7201 ctcctccgct ccctcgaaca tctgcgccat gtcggtgagc gtcgcgacta cgggagggga
     7261 gatcaagagt acgatgggag cgccgccatc aggcccgcac ccgctcctca acatcatgtc
     7321 ggccaggctt gctgccccct gcgcgatgtc cgaggccgag ctgccgaagc gcgccttgag
     7381 gtcgttggtg ccgagcatga gcgtaacgag atcaagggga cggtgcgact ccagacacgc
     7441 catcaggtac gttctgccgt tcttgtgatc cccctcgatg ggatcgtccc tcaccgtcgt
     7501 cctgcccggc aagccctcct cgatcacacg gtattcctca ccaagcctct tcctgagcac
     7561 cccaggccag cgcacgtcct ccggaaaccg ctctctcgtc tcagggtcag agccccacgt
     7621 gttcgagtcc ccgtaacaca gtatcgttct cacagattcc cctctcgctc ggaagacacg
     7681 aactcattgt tggcgtgatc cacaggccgg gtcaacagca gaccatccgg agatacggac
     7741 aaaggacgga tgtttgatgc ccaaagtcgt catgatatcc ctggaaccgt ccactctagt
     7801 cgaataggtc ctacgtatct gttgacttgc gcaacccgtt cgggtaatgt cggatgcaga
     7861 gggcgaggaa agctgagtgg ggcggggaag ggagcatagg gatggttacc ggtcatcggg
     7921 tagctggtga acgggaggag cctctctccg cggttctcgt ggagagggcg atgaatcgca
     7981 gggacttctt gcggatgagc ggcgcctgtc tcgccggggt cggcttgctc ggcgtggggg
     8041 cttgcggagg gggcggcacc tcagggacgt tgcgctggtc gatgtggtct gacacacctg
     8101 aggagaccag ggtttggaaa gacctcgcca gggacgtaca cgagaagtac ccggatataa
     8161 cggtcaagct agagaccgtc accttcgcgg actactggga caagctcacc acgcagatag
     8221 ccagcgagaa tgaggcggac atcgtcgcca tgcagagtct caggatgccg ggctacgccg
     8281 cgcgcggcgc tttgcaaccg ctgatgcctt ttatcgagga cgactcggac ttcgatgtag
     8341 atgacttctt cggacccatc aggcagggtc tatccgttaa cgacgaattg tacgccctcg
     8401 ggtacgacat aggcccgatc atcctctact acaacaagga tctcttcggc aaggccaaag
     8461 tagagccgcc accggccgag acacccatga gctgggagga gttcagggag aaggccgagc
     8521 agctgactaa ccctggcgcc agggagtacg gattcgtcga gcagcccgtc ttcgacttca
     8581 tggtcccctg gctatggtcg ggcgggggag gctacatgaa cgcagcacag acagagtgca
     8641 cgctcgatac ccctgagtcc ataggggcca tggagttcgt tatcggtatg atcacggaca
     8701 aaatagccgc gcctgtaacc gacctcgcga acccgagctt cgcggtcgag gaattcttcg
     8761 gcgggaagat cgggatgcac gtcgacggtc cctggcagtt cataaacata agggagaact
     8821 ccgatttcga gtgggacgtt gcgccgatgc cggcgggacc ggcggggagc gtgacgtggg
     8881 ccgcaggttc tggattcggc atctccaaca ccacagagaa cgccgacgcg gcgtggacgg
     8941 cattaaagac cataacaagc acggagtcgc tggagaaact cgtcaaggcc ggacgcggct
     9001 acccggcccg cgagtcggcg gtatcgacct tcgagaagcc ccccccgcag aacgtcgacg
     9061 tcgtccagaa gatactcgtc agcgacatcg gcgagaccag gcctttcgtg actaccacga
     9121 cctggcagga gacgacggtc atgctcacgc gcgacttcag ccccgtgttc ctgggcaagc
     9181 agagcgtcga cgcggtcgta agcaaggtca aaccgcaatt cgacaagctc ctaaaggagc
     9241 accaggagat cctcgagcag cagtcgtagc gggagggggt tgtgtggctg gcacggcgca
     9301 aaccgggcgg gggccggaga aagttccctg gaagaaccgc gaacatgaag gactggcggc
     9361 cggcctcttc ctgctgccga acctgatcgg gttcctcatc ttcacggcca tacccgtcgg
     9421 agccgctttc gtcctcgcgt tctacgatta cgatctgctg ctcggggcca gttgggcggg
     9481 cctggagaac ttccgcgagc tgttcaccgt cgacagggtc ttccgggcgg cgctcttcaa
     9541 cacggtctac ttcacggctg tcagcgtacc gctctcagtc gtgctggggc tcgcgaccgc
     9601 gatcctcgtc aaccaggcgc tgcgcggagt tgtgatcttc aggtccatct tcctgctgcc
     9661 ctatgtcacg gtgaccgtgg cgctctcact ggtttggaag tggctctacc tgccggacat
     9721 agggttgatc aactccgtgc tcggcgcggt cggcatcgac ggtccggcgt ggctcacgag
     9781 ccagacctgg gccatgcctg cgctcatcct gatgagcgtc tggaaggggt tcgggtacaa
     9841 catggtgatc ttcctggccg gcctgcaggg cataccggac cacctctacg acgcggccaa
     9901 ggtcgacggg gcgacggcgt ggcgcaggtt tctaaacgtg accctgccgc tgctctcgcc
     9961 gaccaccttc ttcgtcgtgg tcatctccgt gatctcctct ttccaggtct tcgaccaggc
    10021 cctgataatg acgaacgggg gtccaggaac cgcgactacc accctcgtcc tctacatcta
    10081 ccagaagggt ttccaatcct tcgacatggg ctatgccgcg gccgtcgccc tggtcctgtt
    10141 tgcggtgatc ttcgtcttca cggtcgtcca gttcctgttc cagcgacgct gggtcaccta
    10201 cgaatagggg taacgagcgc atgagtgcct tcgactctat cggcgcggct ctcaaaggta
    10261 gggcaggcgt gtcccctggc gggaagcctt actggcccat actcatctat gcgaccctga
    10321 tggcgatggc ttttctgacg ctggttccga tcatctggat ggtactgacc gcgttcaagt
    10381 cggcacccga ggtcgcggcc aacccgccga cctggtggcc cagggagtgg catcccgaga
    10441 acttcgcggc ggcctggaac ttcgccccct tcggcaggta ccttataaat agcctcgtaa
    10501 tggtcggggg catcaccatc ctccagacgg tgacctcggc ccttgccgcc tacgccttcg
    10561 cccgcctgcg attcaggggg cgagatctgc tcttcctcct gtacctgggt acgctcatga
    10621 tcccacccca ggttacggtc ataccgcagt tcatcctcat aaaggaactc gactgggtgg
    10681 atacttacca gggcctcata gtcccccagg cgttcaccgc gttcggggtc ttccttttgc
    10741 ggcagttctt cctcaccatc ccgtacgagc tagaagacgc cgcccgcatc gacggcgcga
    10801 gccgcttcgg ttgtttctgg cgcatcatcc ttccactctc gggaccggcg ctcgccacgc
    10861 tcgccgtctt cgcctttctg ttccactgga acaacctgct gtggccgctg atagtctcca
    10921 actccgacgc gacgatccct gtcgtcgtcg gcctgaggaa cttccagggc cagtacggga
    10981 ccgagtggaa cctgctcatg gccgccgccg ccatagccac catcccgacc gttatcgtgt
    11041 acctgttcgc ccagcgctgg ttcgtccggg gaataaccat gtccggtttc ggcggcaggt
    11101 agcacatata gtcacagata aggaggagca gtgaagcagg agctaggtcg aactaccgcc
    11161 gccgacacag agtggtttac gcgcgaccgc ttcggcctgt ttatccactg ggggatctac
    11221 gccctccccg cccgccacga atgggtcaaa cagcgcgaga ggattcgcga cgagggctac
    11281 cagaagtact tcgaccactt ctaccccgac ctctacgacc ccgaaaagtg ggccagagag
    11341 gcgaggaacg ctggcatgag gtacttcgtc gtcacgacca agcaccacga cggcttctgc
    11401 ctctgggata cggatctcac cgattacaag gcgaccaaca cgctctgggg cgaggacctc
    11461 atcgctccga tggtcgaggc gttccgcgcc gagggcatga aggtaggctt ctaccactcc
    11521 ctcatagact ggcaccatcc cgagttcccg gtcgacgggc tgcactccag gcgagatgac
    11581 acagagtaca tcgaggccaa tagggaccgc gacatctcgc gctaccgcga gtacctgcac
    11641 ggtcagaccc gtgagctcct gacacgcttc ggccgggtcg acgtcatgtg gttcgacttc
    11701 tcctactcgg accgggtctg gggcggcaag ggcaaggagg actggggctc agtagaactt
    11761 atggagatgg tacgcgggct ccagccaggc ataatcgtca acgaccggct ggagatcggg
    11821 ggcgacctga aaacccccga gcaataccag ccgaggagct ggcccgaggt cgaaggagag
    11881 cggaccctct gggaggcgtg ccagaccctg aacggttcgt ggggctatga ccgcgacaac
    11941 ctggactgga agcctactga tatgctcgtc aggatgctcg ttgataccgt ctccaagggc
    12001 ggcaacctgc tcctcaacgt cgggccgaac gcccgcggcg agctcgagtc tcgggccgtg
    12061 gaaagactac gcggcctcgg ggagtggatg cggttgcacg agcgctccgt aaggggttgc
    12121 acggcgagcg acctgacccc gccccccgac tgccggtaca cggagaatgg taacagactc
    12181 tacctgcacc tcttcggctg gcccttcaaa cacgtccatc tcgaagggct ggctgggagg
    12241 gtggagtacg cgcaactcct caacgacgcc tcggagataa agatgaaggt catagaccct
    12301 gcccagcggg cccagaacac gaccgtgggc ggtatcccgg agggtacgct gaccctggag
    12361 ctaccggtgc agaggccgcc cgtcgaggtg cccgtcgtcg aactattctt gaagggatga
    12421 tcggtgtggg cagactccgg ctcaggcagg tccacctcga tttccacact tcggcagagt
    12481 gcgagggtgt cggtgctgat ttcgaccccg aggtcttcgc acagacggta aggatggggc
    12541 gagtagactc catgacggtc ttcgccaagt gccaccacgg cttctcttac tacgcgacgg
    12601 aggtaggcac gagacacccc aacctagccc tcccggatct catgggcgcc cagatagagg
    12661 cgctgcacgg gatcggggtg ctggccccga tctacgtcag catcatgtgg gatgacctgg
    12721 ccggcgagaa ggagcccggc tgggtgatag cccgcaggga cgggagcctc atgatccgtc
    12781 cccccctctc caacgagtcg cccctggcgg ggggatgggg atggaccacg ctggacgtct
    12841 ccactggata cggtgactac ctcgtggcgc agaccgaaga gatctgtgac cgctacgacg
    12901 tagacggctt cttcttcgat atttgcttcc cggttccgaa ctactctccg tggggacagg
    12961 caaagatgcg cgaggccgga gtcagcctcg acgacgagca ggcggtgctg cgttttgccg
    13021 agtggaagtt gcacggtttc ctcgaaaggc tgagcgaggc ggtccgtgcc aaggtcccgg
    13081 gggccacgat cttctacaac ggcaccgtca ccccgcggat ggcagagacg ctcccctacc
    13141 agacccactt cgagatagag agccttccca cctcctcggg gctctggggg tacctgcact
    13201 accccgtcat ggcgcggcag gcccgcacct acggggtcga cttcctcggg atgacggggc
    13261 gcttccacaa ggcctgggcc gacttcggcg gcctgaagac cagcgaccag ctcgactacg
    13321 agtgcggcac catcctcgcc gctgggggtg agatctccgt cggagaccag ctccacccaa
    13381 gaggcgtcct cgaccccgcc gtataccgcc tgctcgagca ttccttcggc aaagtggaga
    13441 gactcgagcc tttcctgcat ggggcgaagc ccacagccga agtggcgatc ctgtcaaggt
    13501 acgagccctc cgccctcggg agtgtcccca cgaacgcgca cgtcgccgaa gtagaaggag
    13561 ccgcgcaact cttcctagaa gcggcgatcc agttcgacat aatagacccc tcgcacggct
    13621 ccttcctgaa ctacgcggcc atcgtgcttc ccgacgggat aagcctgtac gaggaccttc
    13681 gcgcgaagct ggaaagttat acgtccgccg gcggcaaact tatcctgggc ggcacggccg
    13741 ccctcgacga ggagacgggc cagttcggcc tcgagagcat ccctgtcgcc tacgaggggc
    13801 gggccccgac ggtaccaagc taccttcggc tcgacgaggc gcttgctggc gagagcgagc
    13861 tggccacaga ctacgactac gtgttctatg acagggcgca cctggttcgt ccgctcgaag
    13921 gcgccaaggt gcgcggggag ctacgcagcg cgctcttcga caggacgtgg gaacacttca
    13981 cgagccacgc ccaggcgccg gtcggcgagt cgttggactc tgcgctcgtg gtagagagcg
    14041 agggcgttct atacttcgcg acgccgctct ttgcggccta ccgcgaccac gactactggg
    14101 tctacaggga ggtcgcttta cacgcgatcc gcgatttcct gccccggcca aagctgaagc
    14161 tctccggacc cggatgggtg gaggctacgc tgcatgagca gcctgcggac ggcgaccatc
    14221 cggagcggcg gatcgtgcac ctcgtcgcct accatccccg ccgcaccacg cagcccgtac
    14281 aacacgtgga ccagtcgtgg cctacctcgg ggctgggtat cgaaatcttg acggatggga
    14341 gcacggtcgg gcgggcatat ctcgcgccgg agaacgaacc tctcgaagtc gaagtggtgg
    14401 acggctacgc gcggatagcg ctgccgccgg tcgggacgca cgccgtggtc gtgctggagt
    14461 aaagcattgg ccctgaccga cgacgccatc acgagcataa agcagatgat cctctctggc
    14521 caggtcgggc cgggcgagaa gctcccgagg gagaaggagc tcgcgaccag gctaggactc
    14581 tcgcgcagct ccttgcggga ggccatacgc gccctcatcc tggctggcat cctcaagact
    14641 cgccagggcg acggaaccta cgtcacgagc ctggaacccc accagctcct tgagactatg
    14701 agcctggccg tagatttcat gcagaaccac accctcgtgg agctattcga ggtgcggcgt
    14761 atgatcgaac cggccgccac cgcactggcc acaagccgca tcaccccaga agccctagag
    14821 cgtctggaag agaccctccg gcgcatggac tcggcagtga gcgtcgagga actggtcgag
    14881 gccgacgatg acttccaccg gacgatcgct agcgctacgg gcaacgcagc cctggccgcc
    14941 ctactccaga acctctacgg caggaccctg cgggcacgta tctggcgcgg cctcgccgag
    15001 gaaggtgcct tggaccgtac caggatctcc caccgctcta tctacggtgc gatctctaat
    15061 agggatgcag agctcgcgcg ttccgccgcc accatgcaca tagccgaagt cgaagcctgg
    15121 ttccgcagcc tgccgaagtt cactccttcg aacgacgagc ggaccgggac acgcgaaaca
    15181 ccgtctcgat agaaggcgca gaacaacatc cctagagttc ggtcgagatt gaaacgtgtt
    15241 gcactattcg gtacaggggt tgtatcctaa taccatggtg ttaggttagt gtaagctggc
    15301 aactcgaagg cgagcaaggg aaggagactt gtggcgcaga gacaggaaag gacggggatc
    15361 gattccaccg tattctggat agcagccatc atttcggctg tgttcgtcct gtggggtatt
    15421 ctctttacgg acaatctggc tgcggtcttc gatgcggtgc tgtggagctt cctggttccc
    15481 aacttcggtt gggtgttcat actgtcatcg ttcggcttcc tggccttttc actgtatctg
    15541 gctttcagcc ggtacggcaa gatccggctt ggcgggcagg acgaggagcc ggagttcagc
    15601 accgtgtcgt gggtggcgat gatgttcagc gcggggatgg gcataggcct gatgttcttc
    15661 ggcgtggccg agcctctctc ccacatgagc tcgcctcccg gtggtacggc ggaggccggg
    15721 accagggggg ccgcccaggt ggcgatggag tactcctact tccactgggc gttccacccg
    15781 tgggcgatct acgcggtgat ggggctggcc ctcgcctact tcgtctttcg caagggtatg
    15841 cccaacctta tcagtacggc gttctatccg cttctgggcg acagggtcta cggcccgcta
    15901 ggtaagacca tagacatcct ggccatcttc gccacgctgt tcggatcggc gacctcgctc
    15961 gggttgggcg ccctgcagat caaccagggc ctgaacgcca tgttcggttt cgggggaagg
    16021 gaagcagtgg ggctggcgat agtggtcata gccatcctga cggcgggctt catactctcg
    16081 gccatctcgg gggtgcataa aggcatccag tggatcgcca acaccaacat ggtcctggcg
    16141 gtgttcctgc tggtgttcgt gttcctcctg gggccgacgg tgttcatcct caacaccttc
    16201 gtcgagtcgc tcggcggcta cctggccaac ctcatcccga tgagcttccg cacggcggcc
    16261 ttcagcgacg gagcgttcgt ctccggctgg accgtgttct actgggcgtg gtggatctct
    16321 tgggccccct tcgtgggcac cttcatcgcc cgcatctccc gcggtcgtac catccgcgag
    16381 ttcgtcttcg gcgtgatcct ggcccccagc gtggtcagct tcatctggtt cgccattctc
    16441 ggcggaacgg ccatcaacct gcagcttacc ggcgcggcgg acatcgccga gaccgccgcg
    16501 aacaaccagc cggcagcgct gttcctcctc ctggagcagt tcccgctgtt ctggctgatg
    16561 gccggcataa cgataatcct ggtggcgctg ttcttcgtca gcggggccga cgcggcctcc
    16621 atggtgatgg ggatgctctc ctcgaagggc aacctgcatc cgcagtcgtg gaacatggtc
    16681 gtctggggcg tcttcaccgg ggcggcggcg gcgatctgcc tgctgtcagg cgcgattcag
    16741 ggctcggtgg acgccgcgct cttatccctg caggccgtgg cgatcgcgtc agcggcaccg
    16801 ttcgtgctca tcctgatcgg gctgtgcttc tccatcctca aagcgctgcg cgaggagcgg
    16861 gtgcccggcg tgacaccagg actcgaggcg cccgagcctg gcagggccat gtcccgtcaa
    16921 cctggcacca cggcgccgca acagatggcg acagagagac ccgaagagca gcgcgggccc
    16981 cgcgattaga aagcctctgg ggatctagac aactctcgag acacagcctg ggcgggtggc
    17041 caaagccacc cgcccatcat atcggagtgg ccaaacacta aaggagggaa gatgggcagg
    17101 acacgcaacc cgtttcgggg gctcatggac cacatgagcg agatggccag gatgcgggag
    17161 tacgccgagg gcggtggaca ggaagaccag agaaggaccc acgcgaccgc ctggatcccc
    17221 acggccgaca tcttcgccaa gggcgaagat ctcgtgatac gttgcgagct cgccggtgtg
    17281 gaacaggggg atgtggaggt ctctctctcc ggcggcgttc tgaccatcga cggggaacgc
    17341 aagggcgaac ctgaagacgt gagttactac gccagagagc gctactacgg gcacttccgt
    17401 cggacgatga acctgcccga aggcataaac agcaacatga tcagcgccga tttccagaac
    17461 ggcttgctgg aggttaccgt gcagggcgga gcagatcatc cggaacccga gcgcatacaa
    17521 atagagagcc gcaccagcta gcgcgggagg aggaagggtc gacccttcct cctcccgcgc
    17581 tagtcagcat ttcagcacgc tccggctgac gcctccgctt atcagcactt cagctcggcg
    17641 cctgcgccgc cggttggcaa gtcagccaag gctcgcatgc aacaagctga aatgctgact
    17701 aggctgacaa gcgaaggcct taaaggcctg agcgtgctga cttgcttctt gactcccgca
    17761 ccggcctgtg ttagttgtgc cgtatacagg aaaacgattc gtcatgcgca acaagactcg
    17821 gatacgtcct tttgggttct tgggtcttct gggactttgg gcggcggtag cgtgacttgg
    17881 agtgatgtat ggagagtagt gcggggtgtg tgatcatcgg ggccggcatc gttggctgca
    17941 gcgtggccga gcatctgagc cggctcggct ggcgggatgt ggtggtcttg gatcaggggc
    18001 cgctcttcga ggctggcggg tccacctccc acgctcccgg gctcatgttc cagaccaacc
    18061 cgtccaagac gatgaccgac ctggccagat acagcgtcgg gcgctacatg gagctcgagc
    18121 tggatggcga gccgtgtggc taccaggtag gcggcataga ggtcgccgag acccaggaga
    18181 ggtggcggga tctccagcgc aagcacgggc tggcgacctc gtggggcctc gaatccgggc
    18241 tgatttcgcc ccaggagtgc gcggagctgg taccggttct ggattcgaac acgatcaggg
    18301 gcggcttcta cgtccccacg gatggcatag ccaaggccgt cagggcttgc gaagcgatgg
    18361 ccgggctggc cggagagcgc ggcctaaagt tctacggcga gaccgaggtc accggcatcg
    18421 aagtcaagga tggtcgtgtg agatccgtcg agacggacag gggtcgtgtg gagaccgagc
    18481 tcgtcgtgag ttgcgcgggg atgtgggggc ccaggatcgg gcggatggtc ggcatgaggg
    18541 taccgctcgt gccgatgcag caccagtacg cgtggaccac gccgctggag gagcttgctg
    18601 gggaggcgcg cgaggtcgtc catcctatcc tgcgccacca ggacttctcg atgtacttcc
    18661 gccacagagg gaaccattac ggtatcggga cctacaagca caggcccatg cccgtctccg
    18721 ccgacgaaat aaggagggat tacaggggcg aaggtgagtt tccctcggtc tgggagttta
    18781 cgccagagga cttcgagtcc tcctggcaag aggcccgcaa gatgcttccc gccctggaga
    18841 gggtggagat cgaccggccg atgaacgggc tgttctcctt cacacccgac ggcgggcctt
    18901 tgctcggcga gtcgaaggag gccaaagggt tctggatcgc cgaggcggtc tggatcaccc
    18961 acgccatcgg cgttggcaag gtgatggccg agtggatcac cgaaggcgta ccgagtatgg
    19021 acatcagcgg cgccgacgtg caccgcttcg agccctacgc cctcagccct tcctatatcc
    19081 tggcccgcag cgcgcagtcg ttccgggagg tctacgacat catccacccc cagcaaccga
    19141 tggaggagcc gcgccccctg cgggccagtc ctttctaccc gcgccagcag gcgctgggcg
    19201 cctacttcct cgaagcttcg ggctgggagc ggccgcaatg gtttgaggcc aacgagtcgt
    19261 tactcgacgg gcatgaggtt tcggaccgcg gagagtgggc cggaaggtac tggtcgaagg
    19321 tcgtcgccgc cgaagccctg gccacccgcg agcgcgtggc gctctacgac atgacctccc
    19381 tgaagaaggc cgaggtggca ggacccggcg ccctggattt cctgcaaggt ctcaccacgg
    19441 gacagctcga caagcctgcc ggcaaggtga cgtacacact catgctcgac agcgatgcgg
    19501 ggatccggag cgacgtgacc gtagcccggc tcggcgagga ccacttccag ctaggcctca
    19561 acggcccgcg tgacatagag tggatggagc gccacctgcc gcaagacggc tccgtacacg
    19621 tacgggatat cacgccgggg acctgttgta tcggggtgtg gggtcccgcc gcccgagatg
    19681 tggtacagag tctctccgag gacgacttct cgaacgaggc tttcgggttc ttcaacgcaa
    19741 ggcgggctta tctgcgtgag gtgcccgtta cggcgctaag gctctcctat gtaggagagt
    19801 tgggatggga gatctacacc tccgcggacc tggggttgag gttgtgggac ctgttgtggg
    19861 aggccggacg gccctacggt atcctcgcca gcggacgcgg cgccttcaac gcgctgcgcg
    19921 tggagaaggg ttaccgcgcc tggggcaccg acatgaccac cgagcacgac ccctacgagg
    19981 cgggcctcgg attcgccgtc aaggaggata aaggcgactt cgtcggccgt gaggcgctac
    20041 tagagcgcaa ggaagccgga ccgcgccgga ggctggtgtg cctggtgctc gacgatccga
    20101 gcgtggtggt catgggcagt gagccggtct gggccgacac ggcggtaggc tacgtgacca
    20161 gcgccgactt cgggtactcg ctcggacaga gcatcgccta cgcctgggtc ccgcccgagc
    20221 acgccggggt ggggacgagg ctggagatcg agtacttcgg tgtgcgccac cgggcgacgg
    20281 tcaccgagga gccgctcttc gatccggcga tgaagaggat gagggcatga tagaaggtta
    20341 cggaccgaat accgaaagga gaaatgttcg atgaacgaca atgtcaatcc cgaccctggc
    20401 attctgcagt acacgaggat attcaagtcc ccgtacttct acggttcgcg caggcatgga
    20461 gtccagaagt acagcgtcta caaccatcac taccacccgc gctactacag ggacccggtc
    20521 gaggaatact ggcagctcct cgaaggcgtt accctttggg acgtcggggg ggccgaaagg
    20581 caggtcgaga tcacgggccc cgacgccttc gagttcacca acacgctaac cccccgcaat
    20641 ctcaataagt gcgccgtagg gcagtgcaag tacgccttca tcaccaacgc cgagggcggc
    20701 ataatcaacg accccgtgct gctgcgcctc gacgagaacc acttctggct ctccctggcc
    20761 gacagcgacg tgctcttgtg ggcgcagggg ctcgcctaca acatggggct gaacgtcgag
    20821 ataaaggagc ccgacgtcgg cccgatgcag gtgcagggac cgaagtccag ggaggtgatg
    20881 accgacctct tcggcgagga gatactcgag gtgccatact actatatggt gcacagggag
    20941 ctcgacggaa tggagctcgt gatctcccgc accggatact ccggggagct gggatacgaa
    21001 ctctacctga agaacgcgag ccgcgacggg ctcaagctct gggaccgggt gctgcaggcc
    21061 ggcgagccgt acgggatctc cccgatcggc ccgtgccaca tccgcaggat cgagggcggc
    21121 atcctggccc ttggctgcga tatgtggtac gacaccaacc cctacgaagt tggatacggc
    21181 tacaaatgga tggtcgagtt ggagcaggag cagcacttcg tcggaaggga agcgctgaag
    21241 aaggtaagcg aagaaggcgt tagccgaaaa ctggtcggtg tcgagctggg gggtagcaac
    21301 gtcggctcgt acaacgacgg ctccatgatc gactacttcc cggtccacac acacggcgag
    21361 aggatcggca aggtcacctc ggcctgctac tcgccgcgcc tggagaagaa catcggtttc
    21421 gccatggtgc ccgtcgagta cagcgagata gggatcgagc tcgaggtcga gcggccagag
    21481 gagacggtct ccgccaccgt ggccgaccgc gtcttcttca aaccggagca tgccgagcag
    21541 actctggacg ccaccggctc ctccaagggg gcctgagaag ggcgaagtgg gccgtgggga
    21601 agaacgtgtc gacggagcag aaaaccgaac tgcgacagcg ggcgaaccgg agattggcag
    21661 agtttctgcg gcatcccggc tacgaggtga tacccctccg ggggactgaa gaagcggtag
    21721 tcgaacacgt cccaaaggac atagcactca cggttacggc atcgcctggc aagggaacct
    21781 gggcaacgat agagctcgcc gagcggttgg cgggacacgg cttcggtgtc gcgccccacc
    21841 tctcggcgcg gctcatcagg gacaggtcgg agctgggcga gatcctgcac aggctgcgcg
    21901 atgccaaaat aaggaatgcc ttcgtcgtcg ctggtgacat cgatgagccc gcaggggagt
    21961 tcgaaggcgc ggccgggctt ctgagggcga tgaatgagat cgggcacgac ctcgacgagg
    22021 tcggcatcac gggctacccg gagagccacc ccaagatcag cgacgagatg acgatccagg
    22081 ccatgtacga taaggcgccc tacgcaacct acatcatcag ccagatctgc ttcgaccccg
    22141 aggtaacctc ggactgggcc aggcgggtgt ggtcgcgggg tgtaaagctg ccgatccgta
    22201 tcggtatgcc aggctccgtg aaccgccaga agctagtgcg gatctccacc agcatcggcc
    22261 tcggcgagtc ggcgcgtttc ttgaacagac agcgtaactg gctgctcaag atgttcctac
    22321 ccggtggcta cagccccgac cgtctgatcg aaggcctgaa gcccggattc gcagaccccg
    22381 aaagcaacat ccagggcctc cacatctata cattcaacga ggtggagacg accgaggcgt
    22441 ggcggcgcga gatgctggag cgcgccgaaa catccgcctg accgaagagg atagccttgt
    22501 caccagaaaa ccccacgtcg gagtatctgg atctcccact gaacgaattc ctcgaactcg
    22561 tggcctcggg cagcccggct cccggcggag gctcagtggc cgccgtggcc gtggccctcg
    22621 ccgcaggg
//
LOCUS       DATMVF010000119         6574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_293111, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000119 DATMVF010000000
VERSION     DATMVF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293111"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VN178_05900"
     CDS             <1..404
                     /locus_tag="VN178_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80540.1"
                     /translation="VSSPAVAARLLLALDALTMAGVLAFALAGNFAFALGTFWFATLV
                     RSVAEPVYLTWINEGLDPSVRATVISMSSQSGALGEASAGPVIGAIGNIFGVRPALTV
                     AALILSPTLLLYVRAMRSGVVDPRAKDAGEG"
     gene            578..1369
                     /locus_tag="VN178_05905"
     CDS             578..1369
                     /locus_tag="VN178_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80541.1"
                     /translation="MYWIYDISHWAGALLFAVVFVGFTCLGTVLIRPWVRQTAADQAG
                     WNTLIGAVYAAYVVFYGITLALIAVSAYQGYVAANQTVGREAAALGTLYRDVSSYPEP
                     IRGELQAMLRDFTRYVIEEAWPAQRRGIIPEGGTARTTAFQNRLLSFQPQTRSEAILH
                     AETIEQFGVFVDYRRQRLHSVLAGLPDALWFVVGVGAMLNAVLTWLFDVNRLKVHLIL
                     AGILSLFVALVVYLVVAMDNPFRGELSVYPEAFQVVQRSLMEPGR"
     gene            2106..2864
                     /locus_tag="VN178_05910"
     CDS             2106..2864
                     /locus_tag="VN178_05910"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWS80542.1"
                     /translation="MHESLRSKHNDRILPGPGALERESDLLEGADPREVIAWAVETYG
                     EGLALSASFGGGEGMALLDMISKVTDKVTVLTIDTGFIFKETAEFREEVMRRYKLPVE
                     VLKPELTIEEQVERFGEQMRTCTPNLCCQIRKIEPLQKALNRYDAWMTGIRREQTLQR
                     AGTKVVAWEERYQAAKIAPMPDWTEEQVWDYVRAHDVPVNPLLHQGYKSIGCEPQTRP
                     VHHDEDPRAGRWAGTDKTECGIHFEGGKISRAGA"
     gene            2904..4604
                     /locus_tag="VN178_05915"
     CDS             2904..4604
                     /locus_tag="VN178_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HWS80543.1"
                     /translation="MKFLREDQTLNKVEKIKQERHPLEVYGAIINTYSKDLSAMDAVP
                     GEVERLKWVGIYPQKQGGDAFMMRIKIPGGVLTPEQTRVIGKVAADFARGPVPNPHFG
                     NNFLDLTTRQDVQMHWIKMENVPEIWRRLEEAGITTVQACGDSARNVLCCPVSGLGHE
                     EAIDAYPVAQAISAFFTGNREYANLPRKFKMSVTGCLEDCAQAEINDVGMLPARLEDG
                     TLGFNLRVGGGLSDGPRMASDIDVFVRPEDAVEITRGIAQVFGELGDRENRWTARMRY
                     LVQELGPEAFREELAKRVSVALTPAGEDLTKRYRGDHIGVHPQKRGGDGARNVDGRRG
                     GFQTRPYTETRPHVYVGLNVPVGRMSGEQFEEAGRLAGEYGDGVRLATDQNLIITGVP
                     EDRLDDLLAEPLLARYSPKPGAFERGVVACTGSEFCRFGIVETKVRALEWAREMDERV
                     GDPGQDAVRMHFSGCSASCAQPQIGDIGFRGETAKTRDAIVEGVDIGLGGSLGKDAAF
                     IDWVEGAKPAGEVPDALVAVFEQFEEERREGERFHEWARRKRNAELREALRKPVGAGR
                     "
     gene            4604..5803
                     /locus_tag="VN178_05920"
     CDS             4604..5803
                     /locus_tag="VN178_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HWS80544.1"
                     /translation="MAGFDIREMMNDIDEAPGKVWFWDLEKAVIDADRCVQCGVCVAA
                     CPTDSIGIGEDDLPELVKMCTGCSLCWDFCPRGGLQYESTWKITGGSNGKSIEGMGRV
                     EESYTARVRKRIDGVQDGGFVSALLISLLESGEIDGALLARESATERWKGEAFLATTP
                     EEVRACAGSFYNQTLALGHVDFDGHDLPPNPRIAVVGTPCEIEGIKAMQARPWTWGGS
                     KVEAITLTIALLCTKSFNYEKLMLEEIRDKRNVDLDSVGRVDVIRGKMIVQDLEGATI
                     FEEPIRDFHGAALKGCDECADFMGHAADISVGSVGSADGYSSVILRSEEGLAAFDSVR
                     DRLELRPLDKPQALDKLDGLDKRVALNSLKRAFDPDAPLFIDYEEHLNDYAGTDRAPV
                     EHEAVRY"
     gene            5831..6145
                     /locus_tag="VN178_05925"
     CDS             5831..6145
                     /locus_tag="VN178_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80545.1"
                     /translation="MPDEPTELKAETRALLEDSPEEGGRVIADAAFVSDLLWEEWGAD
                     LEGAGMDYGRFLEISRSYAGEFRFWVVGERPWEHCAAGLAGRLLRRLPAGQVTNLVEV
                     NG"
     gene            6142..>6574
                     /gene="sat"
                     /locus_tag="VN178_05930"
     CDS             6142..>6574
                     /gene="sat"
                     /locus_tag="VN178_05930"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF025664.4"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HWS80546.1"
                     /translation="MNVSTEYTTIAPHGGELVDRRAPEEQRGELLNRAEGFARVTLAP
                     RALSDLEMLSTGVFSPLPGFMVSEDYESVVETMHLKDGLAWSLPITLPASEEEANGIR
                     VGEEISLTDEAGRVLATMIVEDRYSYDKEHEAREVYGTTNPE"
BASE COUNT         1264 a         1977 c         2191 g         1142 t
ORIGIN      
        1 atgtaagcag cccggccgtc gccgcgcggc tgcttctcgc gctcgatgcg ctcaccatgg
       61 caggggtgct cgccttcgcg ctggccggca acttcgcgtt cgcgcttggg acgttctggt
      121 tcgccaccct ggtccgaagc gtcgcagagc cagtctacct gacctggatc aacgagggcc
      181 tggaccccag cgtccgggcc accgtgatct cgatgagcag ccagtcaggc gcccttggcg
      241 aagcctcggc cggacccgtg atcggggcca taggcaacat cttcggcgtc aggccggccc
      301 tgaccgtcgc ggccctcatc ctctccccga ccctcctgct ctacgtacga gccatgagaa
      361 gcggggtagt cgatcccaga gccaaagacg caggagaagg atagtagggg tgccgatagg
      421 aacggaaccc ggactaacga ccttaccgcg gcttgtttaa gtagcgaacg ctgcatgaag
      481 tctcgacctg cactaggatt tgtcagttag cacctatctt agtgcaagac cgcaacaccg
      541 ggccacagca gcacgagacc cgatagggaa ggccaaaatg tactggatct acgacatatc
      601 tcattgggcg ggagccctcc tgtttgccgt tgtctttgtg ggcttcacct gtctgggcac
      661 cgtcctcatc cgtccctggg tccgccagac cgccgccgac caagccggtt ggaacacgtt
      721 gataggcgcc gtctacgctg cctacgtagt gttctacggc atcacgctcg ccctgatcgc
      781 cgtctccgcc tatcagggct acgtagccgc caatcagacc gtcgggcggg aggcagcagc
      841 cctcggtacg ctctaccggg acgtctccag ctacccggag ccgatccgcg gagaactcca
      901 ggccatgctc cgcgatttca cccggtatgt gatcgaagaa gcctggccgg cccagcggcg
      961 ggggatcatc cccgagggag gaaccgcgcg gaccaccgcg tttcagaata ggctcctgtc
     1021 cttccagcca cagacgcgat ccgaagcgat cctgcacgcc gagaccatcg agcagttcgg
     1081 cgtcttcgtc gattatcgtc gccagcggct gcacagcgtg ttggcggggc tgcccgatgc
     1141 gttgtggttc gtcgtcggcg tcggtgcgat gctaaatgcg gtgctgactt ggctgttcga
     1201 cgtcaaccgg ctgaaggtgc acctgatcct agccggcatt ctgtccctgt ttgtcgctct
     1261 ggtggtctac ctggtcgtgg cgatggataa cccgttccgg ggagaactca gcgtatatcc
     1321 cgaggcattc caggtcgtgc aaaggagcct gatggagcca ggtcggtagc cggtccccga
     1381 gcacctgcgg acggtccgct cccgggccga cgagtagttc gcgcagttga tgaggtcact
     1441 tgctgggttg cggccggatg gtttggtcac tgtggatagg attttgaggt gcagcacgcg
     1501 gagcccgtgt cggaggcgag ctcctagttc tggccttcgg gactccttgc cgtgccgccc
     1561 cgcgagtacc gcacgatgcg ctagctgcct gtcccgctgg cgccatggtg ggccaggccg
     1621 atagcggcga aaggccgacg aggcggcggt gctcgatgag ctgcgcgcgc caccctgaac
     1681 ttctcagccg aggaacggtg ggtgcatctg ccagatctgc cggcaggcag cgtgattcat
     1741 tcgaccttga tcgcgaggga gtcacggatc cggcgaagag ctcctccttc gtggccacga
     1801 aggcgtagtc gtcgaactca gcggacgagc aagttgccac cgccttaccg agtagccggc
     1861 cagggagatc ccttactccc cgtaatggag gagaattctt tcaattccga tgaaaattat
     1921 cgggtttttg ttgacgtacg gttgttcggc gcttagtatc cggcgcatgg agttacacgg
     1981 tacgcagggt catgcgaagg gccgaatgtc tttggtccct cgcgcgggcg cgcggcgatg
     2041 tagcatgtgc gcttgatctc agcccttgac tttccattac acgacttaca ctggaggatc
     2101 gcgcgatgca cgaatccctg cgttcgaagc ataacgacag aatattacct ggtccggggg
     2161 cgctcgagcg ggagtccgat ctgctggagg gcgcagaccc gcgggaggta atcgcgtggg
     2221 ccgtggagac ttacggtgag ggcctggccc tctcggcctc gttcggcggg ggcgagggga
     2281 tggcgctgct ggacatgatc tccaaggtca ccgacaaggt caccgtactc accatcgaca
     2341 cgggctttat cttcaaggag actgccgagt tccgcgagga agtcatgcga cgctacaaac
     2401 tgcccgtcga ggtcctgaag cccgaactca cgatcgagga gcaggtcgag cgcttcggcg
     2461 agcagatgcg cacctgcacc ccgaacctgt gctgccagat ccgcaagata gaacccctcc
     2521 agaaggcgct gaaccgctac gacgcctgga tgacgggcat tcgcagggag cagaccctgc
     2581 agagggcggg cacgaaggtc gtggcctggg aggagcgcta ccaggccgcc aagatagcgc
     2641 ccatgcccga ctggacggaa gagcaggtat gggactacgt gagggctcac gacgtgcctg
     2701 tcaacccgct cctgcaccag ggttacaaga gcatcggttg cgagccgcag acgaggcccg
     2761 tgcaccacga cgaggatccc agggccggcc ggtgggccgg aaccgacaaa acggagtgcg
     2821 gcatccactt cgaaggcggc aagatcagcc gcgcgggtgc ctgaatctca gagatccctc
     2881 tccaaaagcg caggttctat agcatgaaat tcctcaggga agaccagacc ttgaacaagg
     2941 tcgagaagat caagcaggag cgccatcccc tagaggtgta tggggccatc ataaacactt
     3001 actccaagga cctctctgcc atggacgccg tccctggcga ggtcgagcgg ttgaagtggg
     3061 tcggtatcta cccgcagaag cagggcggcg acgcgttcat gatgcgaatc aagatccctg
     3121 gcggcgtcct gacgccggag cagaccaggg tcataggcaa ggtagcggcc gacttcgcgc
     3181 ggggccccgt gcccaacccg catttcggga acaacttcct cgatctgacc acgcgccagg
     3241 acgtgcagat gcactggatc aagatggaga acgtgcccga gatctggcgc cgcctggagg
     3301 aggccggcat cacgaccgtg caggcctgcg gcgactcggc ccgcaacgta ctctgttgcc
     3361 ccgtctcggg actcggtcat gaagaggcca tagacgccta ccctgtggcc caggccatct
     3421 ccgccttctt taccggcaac cgcgagtacg caaacctgcc gaggaagttc aagatgagcg
     3481 tcacgggatg cctggaggac tgcgcgcagg ccgagatcaa cgacgtgggc atgctccccg
     3541 cccgcctgga agacggcacg ctgggcttca accttcgcgt cggcggcggc ctctcggacg
     3601 gcccgaggat ggcctcggat atcgatgtct tcgtcaggcc cgaagatgcg gtggagatca
     3661 cccgcggcat agcccaggtc ttcggcgagc tcggggacag ggagaaccgc tggaccgcga
     3721 gaatgcgcta cctggtccag gagctcggtc cggaggcctt ccgcgaagag cttgcaaagc
     3781 gcgtttccgt cgcactcaca cccgccggag aggacctgac gaagagatac cgtggcgacc
     3841 acatcggggt ccacccccaa aaacgcgggg gcgatggtgc gcgtaacgtg gatggtcgta
     3901 ggggcgggtt tcaaacccgc ccctacaccg aaacccgccc gcacgtttac gtcggtctaa
     3961 acgtacccgt cggaaggatg agcggcgagc agttcgagga ggcggggagg cttgccgggg
     4021 agtacgggga tggggtccgc ctcgctacgg accagaactt gatcattact ggtgtccccg
     4081 aggatcgtct cgacgatttg ctggccgagc cccttctggc acggtactcc ccgaagcccg
     4141 gggcgttcga gcgtggcgtt gtggcctgta cggggagtga gttctgccgc ttcggcatcg
     4201 tcgagaccaa ggtcagggcc ttagagtggg cccgcgagat ggacgagagg gtcggggatc
     4261 ctggccagga tgcggtgagg atgcacttct cgggctgctc ggcctcctgc gcccagcccc
     4321 agatcgggga cataggcttc cggggcgaga ccgccaagac gcgggacgcg atcgtggagg
     4381 gcgtggacat agggctcggc ggctcccttg gcaaggatgc cgcgtttatc gactgggtgg
     4441 agggcgcgaa gccggccggg gaagtccccg atgccctggt tgccgtcttt gaacagttcg
     4501 aggaggagcg gcgagagggc gagcgcttcc acgagtgggc caggcgcaag cgtaacgccg
     4561 agttgcgaga ggcgctgcgc aagccggtcg gagctggtcg atagtggccg gcttcgacat
     4621 cagggagatg atgaacgaca tcgacgaggc ccctggcaag gtctggttct gggacctgga
     4681 gaaggcggtg atcgacgccg accgttgtgt gcagtgcggc gtgtgcgtcg cggcctgccc
     4741 gaccgactcc atcggcatcg gcgaggacga ccttccggag ctcgtcaaga tgtgcaccgg
     4801 ctgctctttg tgctgggact tctgcccccg cggcggcctg cagtacgagt cgacctggaa
     4861 gatcaccggc ggctctaatg gtaagagcat cgagggcatg ggacgcgtcg aggagagcta
     4921 cacggcccgc gtcaggaaga ggatagacgg cgtgcaggac ggtggcttcg tcagcgcgct
     4981 tctgatctcg cttctcgagt ccggcgagat agacggcgct ctcttggcgc gcgagagcgc
     5041 gaccgaacgc tggaagggcg aggcttttct cgcgactacg cccgaagagg tgcgcgcttg
     5101 cgcggggagc ttctacaacc agacgctcgc gctggggcac gtcgacttcg atggccacga
     5161 cctgccaccg aaccccagga tagcggtcgt ggggacccca tgtgagatcg agggcatcaa
     5221 ggcgatgcag gcgaggccat ggacgtgggg cggctcgaag gtcgaggcca taacgctaac
     5281 catagccctg ctctgcacca agagcttcaa ctacgagaag ttgatgctag aggagatcag
     5341 ggacaagagg aacgtcgacc tggatagcgt aggacgggtt gacgtcataa gggggaagat
     5401 gatcgtccag gatctcgaag gcgcgacgat cttcgaggag cccataaggg acttccacgg
     5461 tgcggccctc aagggttgcg acgagtgcgc ggacttcatg ggtcatgccg ccgacatctc
     5521 agtggggagc gtcgggagcg cggacggtta ttccagcgtg atactccgca gcgaagaagg
     5581 cctggccgcc ttcgacagcg tcagggatag attagagctt cggccccttg acaagccgca
     5641 ggcattggat aagctagacg gcttggacaa gagggtggcc ttgaatagtc tcaagcgggc
     5701 cttcgacccg gacgccccgc tgtttataga ctacgaggaa catctgaacg actacgccgg
     5761 gacagaccgc gccccggtgg agcacgaagc ggtgaggtac tagttggaag gcgatgtaaa
     5821 gaggctctct gtgccggatg agccaacaga gctaaaggca gaaacccgtg ccctactcga
     5881 agattcgccc gaggagggtg gccgtgttat cgccgacgcc gcctttgtct cggacctcct
     5941 ctgggaagaa tggggggccg acctcgaagg agccggcatg gactacgggc ggtttctgga
     6001 gatctcgcgc tcttacgcgg gcgaattcag gttctgggtc gtcggcgaac gtccctggga
     6061 acactgcgcg gccggcctcg ccggtcgcct gttgcgtaga ctccctgccg ggcaggtcac
     6121 taatctcgtg gaggtaaacg gatgaacgtc agcaccgaat acactaccat cgccccgcac
     6181 ggcggcgagc tcgtcgaccg cagggcgccc gaggagcaac gcggagagct actcaacagg
     6241 gccgagggct tcgccagggt aaccctggct cccagggctc tctctgatct ggagatgctc
     6301 tccaccggcg tcttctctcc cctccctggc tttatggtca gcgaggacta cgagagcgtc
     6361 gtcgagacga tgcacctgaa ggacggcctg gcctggagcc tgccgatcac actccccgcg
     6421 agcgaggaag aggcgaacgg cataagggtt ggcgaggaga tctccctcac cgacgaggca
     6481 ggacgcgttc ttgccaccat gatcgtcgag gaccgttact cctacgacaa ggagcacgag
     6541 gccagggagg tctacggtac gacgaacccc gaac
//
LOCUS       DATMVF010000120         5024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_608959, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000120 DATMVF010000000
VERSION     DATMVF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608959"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /gene="tuf"
                     /locus_tag="VN178_05935"
     CDS             <1..142
                     /gene="tuf"
                     /locus_tag="VN178_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWS80547.1"
                     /translation="EMVMPGDNTVMNVELISPIAMDEGLNFAIREGGRTVGAGVVTEI
                     VE"
     gene            201..350
                     /gene="rpmG"
                     /locus_tag="VN178_05940"
     CDS             201..350
                     /gene="rpmG"
                     /locus_tag="VN178_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWS80548.1"
                     /translation="MRQLVTLACEECKRRNYHSRKNRRNTPDRIQLQKYCPWCRHHTA
                     HRETR"
     gene            365..437
                     /locus_tag="VN178_05945"
     tRNA            365..437
                     /locus_tag="VN178_05945"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:398..400,aa:Trp,seq:cca)
     gene            468..716
                     /gene="secE"
                     /locus_tag="VN178_05950"
     CDS             468..716
                     /gene="secE"
                     /locus_tag="VN178_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWS80549.1"
                     /translation="MGKKRGSAGSGKKQPQSKGGGFLAGPRRFTRDVRSELRRVSWPD
                     RDQLRQSTAVVLIIVIVLALYIAAVDVVFQSLVRLVFL"
     gene            748..1278
                     /gene="nusG"
                     /locus_tag="VN178_05955"
     CDS             748..1278
                     /gene="nusG"
                     /locus_tag="VN178_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWS80550.1"
                     /translation="MKKWYVVNTYSGHENKVRTTLERRIDSMGLERNFGEIRIPTESV
                     IEIKDGKKVPTIQRQFPGYVLVNMDMNDTTWQLVRQTPGVTQIVGAGEKPLALSRAEV
                     ERLLHPGEVETREKVKTTVDYQIGETVKVIGGPLSDFTGSISDINVDQSRLKVLVSIF
                     GRETPVELSFNQVAKL"
     gene            1292..1729
                     /gene="rplK"
                     /locus_tag="VN178_05960"
     CDS             1292..1729
                     /gene="rplK"
                     /locus_tag="VN178_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWS80551.1"
                     /translation="MGRGRGRRVARKLKLQITGGQANPAPPVGPALGQAQVNIGEFVR
                     QFNDATRDQMGQIVPVEITVYEDRSFTFITKTSPAAFLLKQAAGLDKGSGEPNRMKVG
                     TVTRAQVEEIARTKMPDLNAADIKGASKIVEGTARSMGITVDG"
     gene            1722..2411
                     /gene="rplA"
                     /locus_tag="VN178_05965"
     CDS             1722..2411
                     /gene="rplA"
                     /locus_tag="VN178_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWS80552.1"
                     /translation="MGRKLLEQKSRIDPEQRYTPREALELLKDLEGAKFDESVEAHVH
                     LNVDPRRADQMVRGTLMLPNGTGKSRRVAVFAEGEKAREAEESGADVVGSDDLAARIK
                     NEGFMDFDVAVATPDQMRAVGQLGPVLGPRGLMPNPKSGTVTQDVGRAVEEIKRGKIE
                     YRVDRYGIIHGVIGKKSFDLDSLVENYFALREELQRARPAPVKGRYFKSVALSTTMGP
                     SVKVDPAVERD"
     misc_feature    2426..2558
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2568..3095
                     /gene="rplJ"
                     /locus_tag="VN178_05970"
     CDS             2568..3095
                     /gene="rplJ"
                     /locus_tag="VN178_05970"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWS80553.1"
                     /translation="MNREEKVRVIESLTAKLRAGSAVLVDYQGMNVARSTDLRRRSRE
                     SGVEFVVAKNTLTKRAADEAGVEGLSEFLVGPTALAFSEDPVASAKLMAEFADQVESF
                     ALKGGLLEGGRVLDEAEVVALSRLPGREQLLAQIVGGISSPLTGLVTVLNNTIQGLVV
                     ALGQIAEQKQSAEES"
     gene            3123..3506
                     /gene="rplL"
                     /locus_tag="VN178_05975"
     CDS             3123..3506
                     /gene="rplL"
                     /locus_tag="VN178_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWS80554.1"
                     /translation="MAVNKEELMESIENMTVLELSELVKALEERFGVSATAVAAAPAA
                     GAAGDGAAAAEEEQTEFDVVLQGPGEKKIQVIKVVRAATGLGLKEAKALVDEAPNPVK
                     EALSREDADALKAQLEEAGAAVELK"
     gene            3903..>5024
                     /locus_tag="VN178_05980"
     CDS             3903..>5024
                     /locus_tag="VN178_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWS80555.1"
                     /translation="MPDLVEIQLNSFQKFLDVGIQKTFTEISPIEDYTGSYAVEFGEH
                     RFEEPPVSVEECMSKDKTYAAPLFVKVRFIVKETAEVREQDVFMGDFPLMTDSGTFVI
                     NGTERVVVTQLVRSPGAYIMEPKDPTKQVLTASLMPARGSWLEFEVEAKGYVSVRIDR
                     KRKLPVSVLLKALGMGGPEEILSRFEDSWLIRNTLQRDDTPSREEALLEVFRRQRPGE
                     PVNIENAQNLVDGLFFDAKRYDLSEVGRYKVDKKLKLDVPLDTHTLTIEDIEALLKRL
                     IETSEEVAEEEEFGRVNYERIRHKLDEYEHFGNRRLRTVGELVQEAFRIGMYRMERVV
                     RERMTTQDEDSITPQSVVNVKPVSSAIKEFFGSSQLSQFM"
BASE COUNT         1062 a         1424 c         1694 g          844 t
ORIGIN      
        1 agagatggtg atgcctggag acaacacggt aatgaacgtg gagttgatca gtcccatcgc
       61 gatggacgag gggttgaact ttgccatcag ggagggtggc aggacggttg gggcaggcgt
      121 cgtaacggag atcgtggagt aacgaaccgt tggtgcacag ggacggtgag gtcgccgtcc
      181 ctgtgatttg gaggtaagtt gtgcgacaat tggtaacgct ggcttgcgag gagtgcaagc
      241 gccggaacta ccacagcagg aagaaccggc ggaacacccc ggatcgcatc cagctacaga
      301 agtattgccc gtggtgccgc catcatacgg cgcaccggga gaccaggtaa ggatcgtcag
      361 acagagggca gtagctcagt tggtagagca gcggtctcca aaaccgcagg tcgggggttc
      421 gagtccctcc tgccctgctt cgagaggcaa agccatgcga ggacacaatg ggtaagaagc
      481 gcggatccgc ggggtcgggc aagaagcagc cgcagagcaa gggtggcggc tttcttgctg
      541 gaccgaggag gtttaccagg gacgtgcgga gcgagctcag gcgggtcagc tggcccgacc
      601 gtgaccagct caggcagagc acggccgtgg tgctcatcat cgtcatcgtg ctggcgctct
      661 atatagctgc ggtggacgtc gtgttccaga gtctggtgcg cctggtcttc ttatagatct
      721 ccctttaggc attaggagcg tttcgttgtg aagaagtggt acgtcgtaaa tacatactcg
      781 gggcacgaga acaaggtgcg cacgacgctg gagcgcagga tcgactcgat gggcctggag
      841 cggaacttcg gtgagatccg catcccgacc gagagcgtca tcgagatcaa ggacggcaag
      901 aaggtcccca cgatccagcg ccagttcccc ggctacgtcc tcgtcaacat ggacatgaac
      961 gacacgacct ggcagctcgt tcgccagacg ccgggggtga cccagatcgt gggcgccggg
     1021 gagaagccgc tcgcgctcag ccgggccgag gtggagaggc tcttgcatcc tggcgaggtc
     1081 gagacgcgcg agaaggtcaa gacgacggtc gactaccaga tcggggagac ggtcaaggtc
     1141 atagggggac cgctctcgga cttcacgggt tcgatctcgg acatcaacgt ggaccagtcg
     1201 aggttgaagg tgctcgtctc catcttcggg cgtgagacgc ccgtagaact ctccttcaac
     1261 caggtagcga agctctaagg agaggattta tatgggtaga ggacgcgggc gccgcgtagc
     1321 gcgcaaattg aagttgcaga tcacgggcgg acaggcgaac ccggctccgc cggtaggccc
     1381 tgccctgggc caggcgcagg tgaacatcgg cgagttcgtg cggcagttca acgacgccac
     1441 gcgcgaccag atgggccaga tcgtccccgt ggagattacg gtctacgagg accgctcttt
     1501 caccttcata accaagacct ctccggcggc gttcttgctg aagcaggccg caggactcga
     1561 caagggcagc ggcgagccaa acaggatgaa ggtcggaacg gtgacgagag cccaggtcga
     1621 ggagatagca cgcacaaaga tgcccgacct caacgccgcg gatatcaagg gcgcgtcgaa
     1681 gatcgtcgag ggcacagcga ggagcatggg gattactgtc gatgggtaga aagctgctgg
     1741 aacagaagtc gaggatagat ccggaacagc gctacacgcc gcgcgaggca ctggagctac
     1801 tgaaagacct cgaaggcgcg aaattcgacg agagcgtcga ggcccacgtc cacctgaacg
     1861 tggaccccag gcgcgccgac cagatggtac ggggcacgct gatgctgccg aacggcacgg
     1921 gcaagtcacg cagggtcgcg gtcttcgccg agggcgagaa ggccagggag gccgaggagt
     1981 cgggggccga cgtggtcggc tctgacgacc tcgcggcccg catcaagaac gagggcttta
     2041 tggacttcga cgtggccgtc gcaaccccgg accagatgag ggccgtgggc cagctcggtc
     2101 ccgttctcgg tccgcgcggg ctcatgccga acccgaagag cggcacggtg acgcaggacg
     2161 tcggcagggc cgtcgaggag atcaaacgcg gcaagatcga gtaccgcgtg gaccgctatg
     2221 gcatcatcca cggcgtgata ggcaagaagt ccttcgatct ggatagcctg gtcgagaact
     2281 acttcgcgct gcgcgaggag ctgcagcggg cccgtcccgc ccccgtaaag ggtcgctact
     2341 tcaagtccgt cgccctctcc acgacgatgg gaccctccgt gaaggttgac cccgcggtgg
     2401 agagagatta gtatcacgca cggactttag tcactagagc aaaccagaga cagccggggt
     2461 ccgagaggac ttaattatcc ggccgaggtt gcggaaagct ttcgagggaa ccaagagccc
     2521 cgagcgcctc tgcggcctcg gggttcttta atcgtagagg agtcggattg aacagggaag
     2581 agaaagtgcg ggtgatcgag agcctcaccg cgaagctgcg ggccggctcc gcggtgctcg
     2641 tcgactacca gggcatgaac gttgccagga gcaccgacct ccgtcgccgc agccgcgagt
     2701 ccggcgtgga gttcgtggtc gcgaagaaca ccctcaccaa gagggccgcg gacgaggccg
     2761 gggtcgaggg gctctcggag ttcctcgtcg ggccgacggc ccttgccttc tcggaagatc
     2821 cggtcgccag cgcgaagctg atggccgagt tcgccgatca ggtggagtct ttcgcgctca
     2881 agggcgggct cctggaaggc ggacgcgtgc tcgacgaggc cgaggtcgtg gcgttgagca
     2941 gactgccggg ccgcgagcaa ctgctcgcgc agatagtcgg cggcatctcc tcgccgctca
     3001 cggggctggt cacggtgctc aacaacacca tccagggtct ggtcgtggcg ctcggccaga
     3061 tcgccgagca gaagcagagc gccgaagagt cgtaagtaaa cagagttagg aggactagag
     3121 agatggcggt caacaaagaa gaactgatgg agtcgatcga gaacatgacc gttctcgagc
     3181 tctcggagct ggtgaaggcc ctcgaagagc gcttcggcgt ctccgccacg gccgtggctg
     3241 cggcccccgc cgccggagcg gccggcgacg gcgccgcagc cgccgaagag gagcagaccg
     3301 agttcgacgt cgtgctccag ggcccgggcg agaaaaagat ccaggtcatt aaggtcgtga
     3361 gggccgccac aggcctcggc ctgaaggagg ccaaagccct cgtcgacgag gcccccaacc
     3421 ccgtcaagga ggccctcagc cgcgaggacg ccgacgccct caaggcccag ctcgaagagg
     3481 ccggcgccgc tgtggaactg aagtagggct cgccctactt cagttccacg ctggtcagca
     3541 cggcgcctga aagaagccgt gtcagcacgc gccctgtcgg gcgcttgtca gcatttcagc
     3601 ttgctaatgt gaaaggccta gctgactagc tgacaaggcg gcgcagccgc cgtgctgaag
     3661 tgctgactag ctccgttcgt ggcatgccac gaacggttgt gctacactcc acgcgccggt
     3721 cacaggtcgg ccacaaatta tctgcgattc tgccttgact cgcacgggta ttcggggtaa
     3781 gatactcgtt tgcgtgttcg gccattcttc aacgacccat gcggaggaga gctaacttgg
     3841 tgagccctgt cgtgcgccgg aaacggcacc cgtatgctcg gttacagtcc acagaagttc
     3901 agttgccgga tctcgtggag atccaactga attcgttcca aaagtttcta gatgtaggta
     3961 tccagaagac ctttacggag atctccccta tagaggacta cacgggctct tacgcagtgg
     4021 agttcgggga gcatcgtttc gaggagccgc ccgtctccgt cgaagagtgt atgtccaagg
     4081 acaagaccta cgctgctccg cttttcgtga aggtccgctt catcgtcaag gagaccgcag
     4141 aggtccgcga gcaggacgtc ttcatgggcg acttcccgct catgaccgac tcgggtacct
     4201 tcgtcataaa cggcaccgag cgggtggtgg tcactcagct ggtcaggtcg cctggagcct
     4261 acataatgga gcccaaggac cccaccaagc aggttttgac ggcgagcctg atgccggctc
     4321 gggggtcgtg gcttgagttt gaggtggagg ccaagggcta cgtctcggtt cggatagacc
     4381 gcaagaggaa gctgcccgta agcgtgctgt tgaaggcgct tgggatgggc ggaccagagg
     4441 agatcctctc ccgctttgag gactcctggc tcatacgcaa caccctgcag agagacgaca
     4501 ccccttccag ggaagaggct cttttggagg tcttcaggcg ccagaggccg ggcgagccgg
     4561 tcaacatcga gaatgcccag aacctggtgg acggtctgtt cttcgacgcc aagcgttacg
     4621 atctatcgga ggtggggcgc tacaaggtcg acaagaagct caagctcgat gtgccgctcg
     4681 atacgcacac gctgacgatc gaggacatcg aggccctcct caagcgcctc atcgagacct
     4741 ccgaggaggt tgccgaggag gaggagtttg gcagggtcaa ctacgagagg atccgccaca
     4801 agctcgacga gtacgagcac ttcggcaacc ggcgcctaag gaccgtgggg gagctggttc
     4861 aggaggcctt taggatcggg atgtaccgca tggagcgggt ggtgcgcgag cggatgacca
     4921 cccaggacga ggattccata acgccccaga gcgtggttaa cgtaaagcct gtgagcagcg
     4981 ccataaagga gtttttcggc tcctcccagc tctcccagtt catg
//
LOCUS       DATMVF010000121         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_158145, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000121 DATMVF010000000
VERSION     DATMVF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158145"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VN178_05985"
     CDS             complement(<1..424)
                     /locus_tag="VN178_05985"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWS80556.1"
                     /translation="MFSRVGEGLSAGYDRLKTNVWSVLQAAVAASLAYFVAEVLVGHE
                     QPFFAPIAAVICLSVTLGQRNRRAVELVFGVAVGLMVADLLVLVIGNGTLQIAVVVLL
                     AMAAAVFFGGGTMLVNQAAVSAVLVVVLQPPEDVFSPER"
     gene            573..1067
                     /locus_tag="VN178_05990"
     CDS             573..1067
                     /locus_tag="VN178_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HWS80557.1"
                     /translation="MGRRRIEPGQGQESVWDYPRPPRLEDVDKKVKVVFGGVTLAYTT
                     RAKRVLETSHPPVYYIPPEDVRLEHLEPSVESSHCEWKGRASYYDVTTEERTERRAAW
                     FYPDPTPPFVDLEDHVAFYPSRMDGCWVGGQKVEAQEGDFYGGWITPDIVGPFKGAPG
                     TWGW"
     gene            1075..1710
                     /gene="pdxH"
                     /locus_tag="VN178_05995"
     CDS             1075..1710
                     /gene="pdxH"
                     /locus_tag="VN178_05995"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HWS80558.1"
                     /translation="MDASSLRKEYTRAGLDKADVDPDPIVQFHEWFEKVTDADLHEPN
                     AMILATASPDGKPSARTVLLKGYDERGFVFYTNYEGRKANEIEANPMCALLFYWGELE
                     RQVRIEGHASRLSGEESDAYFAGRPRGSRLGAWASEQSRPVEDRSVLEERVRALEAEY
                     EGREIPRPPFWGGYRVVPEEIEFWQGRENRLHDRLVYRREHGAWRIERLQP"
     gene            1824..3014
                     /locus_tag="VN178_06000"
     CDS             1824..3014
                     /locus_tag="VN178_06000"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWS80559.1"
                     /translation="MKEGVKESADRVLRGAVEQENGVPGVVAMATDREGNFYEGAAGK
                     RELGGEQEMTTDTVFAIFSCTKAITGVAVKQLVEEGVISLDHPAKEYAPEISDVQVLE
                     GFDADGEPRLRPPASDVTVEQLMLHTAGFGYDFFNEDLVKYDEKKDVPSVVTSSMASL
                     NSCLLFDPGEQWEYGSNIDWAGKVVEGARGKRLGEVMKERIFEPLGMNDTAFTLRDDM
                     RSRRASIHQRGEDGTLTPMPGFELPSAPEQHMGGHGLYSTVGDYMKFIRMILNGGEGE
                     YGRVLTPEMVEMMGQNGLGDMKIKALPGVIGELSNDAEFFPGMPKSWGYTFMINDHDA
                     PTGRPAGELGWAGLANLYYWIDRKNGLGGFWATQIFPFADPVSFGGCMDFETAVYESA
                     LSAV"
     gene            3369..4193
                     /locus_tag="VN178_06005"
     CDS             3369..4193
                     /locus_tag="VN178_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWS80560.1"
                     /translation="MPLKLVTYSLSGGESRVGSLEGEEIRPLTHQDMIEFIEYGGSPE
                     PGEDTVSLGEASIHAPIARPQKLICIGLNYEDHAAETGADIPEKPIVFAKYPNTIIGP
                     GEAIRIPPITEQADYEAELAVVIGRTARNVSQAEALDYVFGYTNANDISSRDLQFSEG
                     GQWTRSKSIDTFCPLGPYIATTDEIGDPQALFIRCILNGEVMQDGTTSKMIFPVAELV
                     SFLSQGMTLVPGDVIITGTPPGVGAARDPQVWLKAGDEVSIEIEGLGTLTNPVETD"
     gene            4197..>4827
                     /locus_tag="VN178_06010"
     CDS             4197..>4827
                     /locus_tag="VN178_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWS80561.1"
                     /translation="MDLLAQLGTILPEDRVVSSGDELEHHGGGVFTYHTPVRPDAVVY
                     PQSRDEVVEILRFSNEHRVPIVPFGQGSSLEAHTLPVHGGISLDLGRMDRILEIRPDD
                     FIARVQPGLTHGALNAALAGHGLLFPVDPGWDASLGGMAGTNASGTNAVKYGVMRDQV
                     LGLEVVLGDATVIRTGGMAMKSSAGYDLTSLFVGSEGTLGVLTELILRLY"
BASE COUNT          984 a         1454 c         1555 g          834 t
ORIGIN      
        1 agcgctcggg cgagaagacg tcctcgggag gttgcagcac tactacgagg accgccgaga
       61 cggcggcctg gttgaccagc atcgttcccc caccgaagaa gaccgcggcc gccatcgcga
      121 ggagcacgac gaccgcaatc tggagcgtgc cgttccctat tacgagcacg aggaggtcgg
      181 ccaccatgag cccgacggcg accccgaaga cgagctcgac ggcgcgcctg ttcctctggc
      241 cgagcgttac gctgaggcag atgacggcgg ctatgggggc gaagaaaggc tgttcgtgcc
      301 cgacgagcac ctcggccacg aagtaggcca ggctcgcggc gacggccgcc tgcaagacgg
      361 accacacgtt ggtcttgaga cggtcgtacc ccgcggagag accctcaccg accctggaaa
      421 acaaacgtcc ttccctcacg ccgcccgcag gaactccgtg aactatacac cctcccgagg
      481 aggccctacg cgccggcaga ttgtgcgcca gcacataaag ggaggatcat cgagtcatat
      541 aatctcagga gttcgaagtc gaggaggttg tcatgggccg caggcgaata gaaccgggtc
      601 agggacagga gtccgtctgg gattacccgc gtccgccgag gctcgaggac gtggacaaga
      661 aggtcaaggt cgtattcggc ggcgtcacgc tcgcctacac tacgcgggca aagcgcgtcc
      721 tcgagaccag ccaccccccc gtgtactaca tcccgcccga ggacgtgagg ctggaacatc
      781 tggagccctc ggtcgaaagc tcgcattgcg agtggaaggg acgcgccagt tactacgacg
      841 tgaccacaga agaacggacg gagaggcggg cggcgtggtt ctatcccgac ccgacgcctc
      901 ctttcgtcga tctcgaagac cacgtcgcct tctacccttc caggatggac gggtgctggg
      961 tcggggggca gaaggtcgag gcgcaagaag gggatttcta cgggggttgg atcaccccgg
     1021 acatagtcgg ccctttcaag ggcgctcccg gtacctgggg ctggtagaga catcgtggat
     1081 gcctcgagcc tccgcaagga atacacgcgc gcaggcctgg acaaagccga tgtagacccc
     1141 gatcctatcg tccagttcca cgagtggttc gaaaaggtca ccgacgccga tctccacgag
     1201 cctaacgcca tgatcctcgc cacggcctcc ccggacggga agccctcggc ccgcaccgtc
     1261 ctcctcaagg gctacgacga acggggcttc gtgttctata ccaactacga aggacgcaag
     1321 gccaatgaga tagaggccaa ccccatgtgc gccctacttt tctactgggg cgagctggag
     1381 cggcaggtca ggatcgaagg ccacgccagc cgtctctccg gcgaagagtc cgacgcctac
     1441 ttcgcgggcc gtccccgagg gagtcgtctc ggggcgtggg cttccgagca gagccgtccc
     1501 gtcgaggaca ggagcgttct ggaagagcgg gtgagggcac tcgaggcgga gtacgagggg
     1561 cgcgagatcc cacgcccccc tttctggggc ggctaccggg tcgtacccga agagatcgag
     1621 ttctggcagg ggcgcgagaa taggcttcac gataggctcg tgtatcgtcg cgaacatgga
     1681 gcgtggagga tcgagcgcct tcagccgtag ttggcttacc ctgcttgaca gcgttgagag
     1741 cgcccggcta gcatatgtcc ttctatccgg ataggggttc cggagcaaac aaagcagcat
     1801 tggctgtggg aatgaggagg gccatgaaag aaggcgtcaa ggaatcggcg gaccgcgtcc
     1861 tgcgcggggc cgtcgagcag gagaacggcg tgcccggggt ggtcgcgatg gcgaccgacc
     1921 gcgaggggaa tttctacgaa ggcgcggcgg gaaagcgcga gctcggcggg gaacaggaga
     1981 tgaccacgga caccgtgttc gccatcttct cgtgtacgaa ggccattacg ggtgttgcgg
     2041 tcaagcaact cgtcgaggag ggggtgatct ccctcgacca ccctgcgaag gaatacgcgc
     2101 cggagatctc cgacgtccag gttctggaag gcttcgacgc cgatggtgag ccgcggcttc
     2161 gcccgccggc ctccgacgtt acggtggagc aactaatgct gcacaccgcg ggtttcggct
     2221 acgacttctt caacgaggac ctcgtgaagt acgacgagaa gaaggacgtc cccagcgtcg
     2281 tcacgtcttc gatggcatcg ctcaattcgt gtctactgtt cgatccgggc gagcagtggg
     2341 agtacggaag caacatagac tgggccggca aggtcgtcga gggggcgagg ggcaagcgac
     2401 tcggtgaggt gatgaaggag cgcatcttcg agccgctcgg catgaacgac accgcgttca
     2461 cgttgaggga cgacatgcgt tcgcgacgtg cgtccataca tcagcgcggc gaggacggga
     2521 cgctcacccc gatgcccggc ttcgagctac cttcggcccc tgagcagcac atgggagggc
     2581 acggcctgta cagcaccgtc ggcgactata tgaagttcat acgcatgatc ctgaacggcg
     2641 gagagggcga gtacggccgg gtgctgacgc cggagatggt cgagatgatg ggccagaacg
     2701 gcctcggaga catgaagata aaggcgttgc caggcgttat cggagagctc tccaacgatg
     2761 cggagttctt ccccggaatg ccgaagtcgt ggggatatac cttcatgatc aacgatcacg
     2821 atgctcccac cggacgtcca gccggcgagc tcggctgggc tggcctggcc aacctctact
     2881 actggatcga tcgtaagaac ggcctcggag gtttctgggc gacccagata ttcccgttcg
     2941 ccgacccggt ctcgttcggt ggctgtatgg atttcgagac ggcggtctac gaaagcgcac
     3001 tctcggcagt ctgaaagcgg tcttcgggaa ggttcaacct tcccgaatac cgcgctggtc
     3061 agctagtcag cacgccgcct cgcttcgctc ggcggcttgt cagcatgtca gcaagtgcat
     3121 gccagtgatg ctcccaggcg tggccaggct ctgtgtcaaa cggtgcggag tcttatctac
     3181 cggcgagcaa tacgaacgca agccgctccc cagcaaagct gaaatgctga ctagctgaag
     3241 tgctgactgg ctcgtcgtgc ataaggccaa aaggccttat gcacgacgct ttgagcttcc
     3301 ccatctgcgc cgtgcggcag ggtccctgct aagatgcgtg tcgttgcgtc tcgaatccta
     3361 ggagaggagt gcctttgaaa ctggtcacgt attcgctgag tggtggggag tcgcgcgtgg
     3421 ggtctctaga gggtgaagag attaggcctc ttacccacca ggacatgatc gagttcatcg
     3481 agtacggtgg tagcccggag cctggcgaag atactgtgtc gcttggggag gcaagcatcc
     3541 acgccccgat agcgaggccc cagaaactca tctgcatagg cctcaactac gaagaccacg
     3601 ccgctgaaac gggggcagat atcccggaga agcccatcgt cttcgccaag tacccgaaca
     3661 cgattatagg gccgggcgag gctatcagga taccccccat aaccgagcag gccgactacg
     3721 aggctgagct cgcagtcgtt atcgggagga cagcccgcaa cgtctcccag gccgaggccc
     3781 tggactacgt cttcggctat accaacgcca acgatatctc ctcacgcgac ctccagttct
     3841 cggagggcgg acagtggacg cgcagcaaat ccatagacac cttctgcccg cttgggcctt
     3901 acatcgcaac cacagatgag ataggagatc cccaagctct cttcatccgc tgcatcttga
     3961 atggcgaggt tatgcaagat ggtacgacat cgaagatgat cttcccggtc gccgagctcg
     4021 tctcctttct ctcccagggc atgaccctcg ttcctggcga cgttataatc acaggcacgc
     4081 caccaggcgt cggcgccgcc cgcgacccgc aagtctggct gaaggctggg gatgaagtca
     4141 gtatcgagat cgagggcctc ggaaccctga ccaaccccgt cgagacggac tagagaatgg
     4201 acctgctcgc ccagctcggc accatccttc cagaggatcg cgtcgtctcg agtggagacg
     4261 agctggagca tcacgggggc ggcgtcttca cctaccacac cccggtccgg cctgacgccg
     4321 tagtctaccc gcagagccgc gatgaggtgg tagagatcct gcgcttctcc aacgagcatc
     4381 gcgtacccat cgtgcctttt gggcagggaa gcagcctgga ggcgcacacc cttcccgtcc
     4441 acggcggcat cagcctggac ctgggaagga tggatcgcat tctggagatc aggcccgacg
     4501 attttatcgc gcgcgttcag cccggcctta cccacggggc gctgaacgcg gcgttagccg
     4561 gacacgggct tctattcccg gtggatcccg gctgggacgc ttcgctgggc gggatggcgg
     4621 gcacgaacgc gagcgggacg aacgcggtca aatacggggt tatgcgggac caggtcctcg
     4681 gcctcgaagt ggtgctggga gatgcgacgg tgatacgtac cggcggcatg gccatgaagt
     4741 cctcggccgg atacgacctg acctcactct tcgtaggttc cgaaggaacc ctgggtgttc
     4801 tgacggaatt gatcctgcgc ctctacc
//
LOCUS       DATMVF010000122        15233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_480047, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000122 DATMVF010000000
VERSION     DATMVF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15233
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480047"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..895
                     /locus_tag="VN178_06015"
     CDS             419..895
                     /locus_tag="VN178_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWS80562.1"
                     /translation="MGRPRNPFRGVMDTMSEMARMREYAEGGGGQEEGRRTHATAWVP
                     TTDIFAVGDDLVIRCELAGVGPDDVEISLSGGALTIDGERKGEPEASEHYTRERYYGH
                     FRRRLKLPENIDANSVSASFENGLLEVTVRGGADHHEPEQIQIKHTPAEETSIRTG"
     gene            928..2112
                     /gene="solA"
                     /locus_tag="VN178_06020"
     CDS             928..2112
                     /gene="solA"
                     /locus_tag="VN178_06020"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWS80563.1"
                     /translation="MNNHEVIVVGCGGLGSAALYWISREIGASVLGIEQFRLGHERGA
                     SQDHSRIIRLAQHQSQYAALAPHAYEAFYEVERESGLQVVLKTGGLVIEDPEGRDPEK
                     VGTRNVDGYVAAFEEHGFDYDLLDSGEVMDRWPQFRLSGSERAIYQPESGLVDARKSN
                     TVHVALARARGANVLENTPVQAVRPNGAGVEVVTDAETYYADRVVVASDMWSNEVLRE
                     TGVQIPLTITQEQLTYYATPNLREFSPERFPVFMWHGTHNFYGFPVYGEVATKLGQHM
                     GGHEVTPETRTFEPDPIRLERYRDFLERHIPGFLGPELYTRTCPYTIPPDQNFVLDTL
                     PEHPQISVAIGAGHAFKFASLIGRVLSELALEGSSTYPIEPFEITRPAITDRTFEKTF
                     HA"
     gene            2112..3407
                     /locus_tag="VN178_06025"
     CDS             2112..3407
                     /locus_tag="VN178_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWS80564.1"
                     /translation="MTGSAADSGRRAVPARSENRGGRATVPKDRGTLGQTNTLLERGV
                     DLERLRRDRLRKVQAEMRTRDVGALLLTDPINIRYVTGVSVMPLWSATNLAHYVLVPA
                     EGSPIVFEFARAKFRAEEFFSDVRNAHYWQARFAEGMAAERSGEWAAEIKDALGGWGV
                     GDDRLGVDCLDYHGFSALQGQGIKLTDADDPIQAARVIKTADEIELLKQSAAVCEAAL
                     YDLEEAICPGVSEHELLGVFYHKMLSLGGEHCFSRLLSTGHKTNPWFHEAGSKLVRPG
                     DLVAFDTDMTGPEGYVCDVSRTFLCGERPTPAQKEAYGVAYEFIQELARRLRPGLGYA
                     ELAEDLPEYPHAYREQRYSFVLHGVGTDDEPPFFPFSDEPGAEKLVGEFRENMVVSVE
                     FYAGKVGEQDGVKLEDEVWVGPDGPVILSLYPYEEELLA"
     gene            3455..4780
                     /locus_tag="VN178_06030"
     CDS             3455..4780
                     /locus_tag="VN178_06030"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWS80565.1"
                     /translation="MTIDRTDHQAAGNPRPRTPEDFTREETYKRTRLPVDLASTLLPE
                     AYTSEEFFAIERERIFASGWIAVGCTSQLDDPGKTLVARVAGRSIIVTRDKAGVLRAF
                     HNVCRHRAAKLLDDGRQMIRNNRIRCPYHSWTYDLEGKCLGTPLFEGSDIPKEQQAIF
                     DMSDVKGFDKADYGLMPVRVDSWGFLVFINLDPDAAPLEEFLGDLPERCSGYRLDEWQ
                     VVREKRYEIASNYKLIGENFMEFYHLPWVHPELVEVSRMEDHYRWQGPGMYMGFCTTP
                     ISQNTDAGGWQGLPPVGSLSGTDAESGRFIWLFPNTAVNILPNHSFIMMTRPEGPSRT
                     IEDTYILAHPESADTPDSEEALDQLAKFWNLVNVQDVEIVERVQEGISNPAYRGGRMC
                     YRFEEPLHRYQNMIIDKMVGVDRVPPGDEEQMSQMFVNPSETPDWTSPE"
     gene            4887..6221
                     /locus_tag="VN178_06035"
     CDS             4887..6221
                     /locus_tag="VN178_06035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS80566.1"
                     /translation="MQLPERSKYVIIGAGVHGLSTAWHLAKELKARGRGSGEDVIVLD
                     KTGIAAGASGIACGTVRNNYFQPAMRELMAYCVSVWESDPEAFAYHPVGYMQISPEVM
                     HEDIVQIYKEQQAIGYPSTLVEGEDECRRYMKNLFHDWRAENITSVLHEHRGGYAHNK
                     ASVRGLAEKAQAEGVRIHTGVRVTGLRLEGGAVEAVETEEGAIHCEQLVVGVGPWIRD
                     VWAMLDLPEKITIMDSEGRLHRDRDMWTYWSLQEGTLGVDPNFLTDNDGAMPPVVHVD
                     TDAPLYDDEGELVTNGLWGIYYKPDFNFGGVQGGAAPHVVDQPAQEVAVDPYGPESPD
                     FVVGEDFARMWTAALSHCQARFEGKRGLFSEEPSGGIGCFTPDSFPVFDTFRQNAYVI
                     ADSNHGYKMIGVGALVAKELMDEPQSLLEPFRFSRYETGKLHPTSHSPFPWS"
     gene            6234..7670
                     /locus_tag="VN178_06040"
     CDS             6234..7670
                     /locus_tag="VN178_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWS80567.1"
                     /translation="MPESRDQLFIANGWTPARDGRTFETIDPSTEEVLATVARGGTED
                     VDDAVAAARAAFDGPWRRVTPQERGRLLYRVARELEARTDEFAELETLDTGKPLGHAR
                     GEISGCVGYLDYYAGMADKIHGETIPLGPDYLNYTVREPLGVTAHIVPWNMPLSMVCR
                     SLAPALAAGNTAVIKPAEQTPLTALKFAEIFLELGFPPGVYNVVTGFGGEAGKALSEH
                     PGIGSITFTGSVETGRSILHAAAEHVKPVVLELGGKSPQVIFADTDLDLAAAEVAKGI
                     YSNTGQYCDAGSRLLVEEGVREPLLERMLEHSRQIELGMGMDDPDMGPLVSAEHLERV
                     LRYIASGQQEGANMLIGGRAEEFERGYFVSPTVFDGVGSKMRIAQEEIFGPVLSVFPF
                     SDEDEAAEMANDSSYGLAAGIFTRDMDRALRFARDIEAGYVMINEYFSGGVGSPFGGY
                     KQSGIGREKGLVALDNYTQIKNVVARVR"
     gene            7725..8945
                     /locus_tag="VN178_06045"
     CDS             7725..8945
                     /locus_tag="VN178_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWS80568.1"
                     /translation="MSDGSETATVVSNLPGIDEKSVRAYRLERVMARLVRHDLAGIVL
                     FDPINVRYATGSRNMQVWAMHNAMRYAFIAPDGPVILFDYPGSSHLSDHLETIDEQRP
                     AVGWDFYGSGARSEEHAERWAAEIADLVVRHGGENKRLAMDRADLLPLRALMSRGVDV
                     VDGKGVMEIARSVKSDEEIRAMRHSIDICARSMTRMREVLNPGVRESEMLATLMYENA
                     RLGGEYPETRLLTSGPRTNPWLQETSDRTLEPGDLVAFDTDLIGSFGFFTDVSRTWLV
                     GEGKPSDEQRRLYTLAREQLEHNAELLKPGLGFLEMTEKSWKMPDVYVPNRYAEIVHG
                     AGMAVEYPLVFYPEDVEASGYDGVFEENMVVCVESYIGAVGGREGVKLEQPVVITRDG
                     SQPLCTYPFEDHFH"
     gene            9092..10138
                     /locus_tag="VN178_06050"
     CDS             9092..10138
                     /locus_tag="VN178_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase family protein"
                     /protein_id="HWS80569.1"
                     /translation="MRAVLLTGHGGPEKLEYREDVPIPEPDRGEVLIEVGAAGINNTD
                     IWTREGAYGAEDDPDKVQGWRREPLKFPRIQGADIAGRIVAVGNGVSEGRIDERVVVD
                     NALYFGDEKELLDCGIVGSERDGGYAEYVTVPAENAHAVFSELSDAELATFPTSYITS
                     LRMLNRVRIAEGETILVTGASGGVGTGLVQLAKLRGAYVIAQVGSGKEEQARRIGADA
                     VVRRDAMDLAAEVREAADGRPVDVVADVVGGPAFAKLLNVLRPMGRYVAAGAIAGPVV
                     SLDLRTLYLKQLELIGSTMGTHEEFAELVNHISSGQLEPLLAKTYPLSQVRQAQEDFK
                     AKSFFGKLALIPSE"
     gene            10307..11053
                     /locus_tag="VN178_06055"
     CDS             10307..11053
                     /locus_tag="VN178_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80570.1"
                     /translation="MIDGGGMLGAPLLFAVELSILYAAGTYAMGHLMAFSGFGGGGNA
                     LGRLAFYLLVFPGVVLHEGAHYLACVLTGTKVTRFAPFSPGRSNDGRLLLGYVRHERR
                     VFPVGAIIGLAPILLNPLGLLLVTALLTPLTLQEVAKPSAEGVVEGLFVSGFLTDTPL
                     LATIWAYLTLSFALGSVPSREDLSSLPLLLVVFGVGIFSISLLRTGSERVLSPAIHDL
                     AALAAGLYALPAIVAIVAALTMGLAGRTLR"
     gene            complement(11236..11661)
                     /locus_tag="VN178_06060"
     CDS             complement(11236..11661)
                     /locus_tag="VN178_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80571.1"
                     /translation="MIVGPSMCRADAPPTSSAGEERRTEQPADHPPRLRVLVAYEDEY
                     RSYRETIDAAIRTLRPHTEVVNAALAELEMMAPRADLIICSRSDVQHTEGTLAWIELP
                     NEPERPAKIFLQGRCSEVTNLTLDGLLAVVDTVADLLLA"
     gene            11930..12619
                     /locus_tag="VN178_06065"
     CDS             11930..12619
                     /locus_tag="VN178_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS80572.1"
                     /translation="MIRILIVEDEVYLARMIARVLGEEGYAAETAETGSSGLTRGLSE
                     RFDLLIVDWMLPDLDGIQVVRGLRSAEVNVPILMLTARTQIEDRVSGLDAGADDYLSK
                     PFAFPELLARVRALTRRPHTPPGMAVVKSGDIVLDPVRHMVWRGQEHIDLTAKEFALL
                     ATLIQRPGQVFTRSLLLATIWAGTRDVYTNVVDLYVSHLRKKLDRAGEPSHIRTVRGI
                     GYAYESRGGAP"
     gene            12619..13869
                     /locus_tag="VN178_06070"
     CDS             12619..13869
                     /locus_tag="VN178_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS80573.1"
                     /translation="MFGRIRLRLTLWYVSILILIVVLFGTIVVLGFRYQVTREQDRLL
                     AREAESKQGIVYGNEPGGANTYSTNSNQIGWTVLEPDGRLLAQTNMGLSLGLPDPALL
                     KETTRERKTSKATVTESGGGVRVLSVPVTRSGEVIGVVQVAQSHEFVEKTVDNFIVRL
                     VPIGLLAVGLAAVGGLFVSGRAMRPVRDAFERQQAFVADASHELKAPLTLIRVDAEVM
                     GRRPRDREDQEILQHLLGETTRMDAILSDLLLLARLDAGETPTVRETFDLTTILTEAA
                     DRFETEAAVEGIGLVVDVEGKLNATGDREYTRQILGALLDNALRYTPTGGSVTVEGRS
                     EDGRIEATVRDSGPGIPPEHLPHVFDRFYRVDAARTRKGGGTGLGLAIARGLARAHGG
                     DLTAANAHGGGAIFNLSLPAAPRH"
     gene            13993..15132
                     /locus_tag="VN178_06075"
     CDS             13993..15132
                     /locus_tag="VN178_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HWS80574.1"
                     /translation="MKANASAVFTWRADRKSTIGRAIQWRAVSRFAHPAIRDDGHRLI
                     KGAATLLVALLVSTSLWSILVVLPAGAELSETGPVDPETGFPVWLEDGKGLRLEPCFT
                     YPNCPQDGRGSSSVASYQGGDVVYWSATSKISNDGAEALLAMSTRGDFREGPVLTEQR
                     VYNRIRIRADGLVPGATYRVTYPYGTETFTNVAGGQRGIDFMEDIGCLQAPCGGFTSA
                     LNGRVGPWLTWTTLASSDDDLPAGYIGDPHKLHEVIGSPVLDSGSEPQNYFKIEGPDV
                     GGPGDDVVRNDLFWLGGKVHGLAVSANPKGGVYDSGSSVTLTSSDPAAKIFYTTDGTE
                     PSADSTPYEGPIQVEAPTTLKYMALGPADEAGNRPRSPVSTETYE"
BASE COUNT         2955 a         4620 c         4997 g         2661 t
ORIGIN      
        1 gggctcaatg gtctgtagac ggtcgccatc ttggccgggg caccattcgc gctgatcatg
       61 atcggcatgg tctactcgct cttcaaagca ttgcgagaag agaagttgcc gtcagccgtg
      121 gtgcagcagc caggcgctgc ccccgagcca gggagggcga tgtcgtcctc tcccagcggg
      181 gccccggccc cacagaggat gtcagcgaag gatcccagag tgccgggtag cggaccttac
      241 actggctgac caaagctccg cgcaagaccg acgcagcggg tcaagagaat tcaaggaggc
      301 tctggtcgcc atccagactc ccagtgcgac aacttaccgg atgccgttcg accagttggg
      361 ttggaggtgt ccgagcccga cgagggttaa cggaataacg agatacgaag gagggagaat
      421 gggcaggcca cgaaacccgt ttcggggcgt catggacacc atgagcgaga tggcccggat
      481 gcgcgagtac gccgaagggg gaggcggcca agaagaagga cgcaggaccc acgccaccgc
      541 ctgggtcccg acgaccgaca tcttcgccgt cggggatgac ctggtgattc gctgtgaact
      601 tgccggcgtg ggacccgacg acgtagagat ctcgctctca ggcggagcgc tgaccatcga
      661 cggggagcgc aagggtgagc cggaggcatc cgagcactac acgcgcgagc gctattacgg
      721 ccacttcagg cggaggttga agctgcctga aaacatagac gcgaacagtg tcagcgcgag
      781 tttcgagaac ggcctgctgg aggtcaccgt gcggggcggg gcggaccacc atgagccaga
      841 gcagatccag atcaagcaca cgccggcgga agagacgagc atccggacgg gctgacgagt
      901 aagaccaggt cgactggggg tatcttattg aacaaccatg aggtaatagt cgtcggctgc
      961 ggcgggctcg ggtcggccgc gctgtactgg atctcgcggg agatcggggc ttccgtgctc
     1021 ggcatcgagc agtttcgatt ggggcacgag cgcggcgcgt cgcaggacca ctcccggatc
     1081 atccgtctgg cccagcacca gtctcagtat gcggccctgg ccccgcacgc ctacgaagct
     1141 ttctacgagg tcgaacgcga gtcgggctta caggtggttc tcaagaccgg cggcctggtc
     1201 atcgaggacc cggaagggcg cgatccggag aaggttggca cccggaacgt cgacggctac
     1261 gtcgcggcct tcgaagagca cggcttcgac tacgatctac tggattccgg ggaggtaatg
     1321 gatcgctggc cgcagttccg cctctccggc tccgagcgcg cgatctatca accggagtcc
     1381 ggtcttgtgg acgcccgaaa gtccaacacc gtgcacgtcg ccctggcccg cgcccgaggc
     1441 gcgaatgttc tggagaatac ccccgtccag gccgtgcgcc cgaacggtgc gggtgtcgag
     1501 gtcgttacgg acgccgagac ctactacgcg gaccgggtgg tcgtggcgag cgacatgtgg
     1561 tcgaacgagg tcttgcgtga gacgggagta cagataccgc tgaccatcac ccaggaacag
     1621 ctgacttact acgcgacgcc gaatctgcgc gagttctccc cggagcgctt tcccgtcttc
     1681 atgtggcacg ggacccacaa cttctacggg ttccccgtct acggcgaagt ggcgaccaag
     1741 ctcggccagc acatgggcgg gcacgaggtc acgccagaga ccaggacctt cgagccagac
     1801 ccgattcgcc tggaacgtta ccgggacttt ctcgaaaggc atatcccggg tttcctcggg
     1861 ccggagctgt acaccaggac gtgcccgtac accataccgc cggaccagaa cttcgttctg
     1921 gacacgctgc cggagcatcc gcagatctcg gtggcgatag gcgcgggcca cgccttcaag
     1981 ttcgcaagtc tcatcggccg ggtactgtcg gagctcgcgc tggaaggctc ctccacctac
     2041 cccatcgagc ctttcgagat cacgcgcccg gccatcaccg accgtacctt cgagaagacg
     2101 tttcacgcgt gatgactggc tctgcggccg attccggacg gagggccgtt cccgcgcgat
     2161 ccgagaaccg tgggggacgg gcgacggtac ccaaggaccg tggcaccctt gggcagacca
     2221 acaccctgct ggagcgtggg gtagacctcg agcgtctccg cagggaccgg cttcgcaagg
     2281 tccaggcgga gatgcggacc cgcgacgtgg gcgcgctgct gctcaccgac cccataaaca
     2341 tccgctacgt tacgggcgtc agcgtgatgc cgctgtggtc ggccacgaac ctcgcccact
     2401 acgtgctggt gcccgcggag ggaagcccga tagtcttcga atttgccagg gccaagtttc
     2461 gcgccgagga gtttttctcg gacgtgagga acgcccacta ctggcaggcc cgcttcgccg
     2521 aggggatggc cgcggagcgc tcgggagaat gggccgcgga gatcaaagac gcgctcgggg
     2581 gttggggagt gggcgacgac cggctcggcg tggattgcct ggactaccac ggcttctcgg
     2641 cgctccaggg acaggggatc aagctcacgg acgccgacga cccgatccag gcggcgcgcg
     2701 tcatcaagac ggcggacgag atcgagctcc tgaaacagtc cgctgccgtc tgcgaggccg
     2761 cgctgtacga tctcgaggag gccatctgcc ccggggtgag cgaacacgag ctcttgggcg
     2821 tcttctacca caagatgctc tccctcggag gggagcactg cttctcgcgg ctcctcagca
     2881 cgggacacaa gacgaacccc tggttccacg aggcgggcag caagctcgtg cgcccaggcg
     2941 acctcgtggc cttcgacact gacatgacgg gcccggaggg atacgtatgc gacgtctcgc
     3001 gcacgttctt gtgcggggag agaccgaccc ccgcgcagaa ggaggcctac ggggtcgcat
     3061 acgagttcat ccaggaactg gctagacggc tgcgacccgg cctgggctac gccgagctcg
     3121 ccgaggacct ccccgagtat ccgcacgcct acagggagca gcgctactcg ttcgtcttgc
     3181 acggcgtggg cacggacgac gagccgccgt tcttcccgtt ttccgacgag cccggggccg
     3241 agaagttggt tggggagttc agggagaaca tggtagtgag cgtggagttc tatgcgggca
     3301 aggtcggcga acaggacggg gtgaagctgg aggatgaggt ctgggtaggc ccggacggac
     3361 cagtgatact ctcgctctac ccctacgagg aggagctgct cgcatgatct cggatacacc
     3421 ttcaggaaga tcctttggag agaggaggtt caggatgacc atcgaccgaa cggaccacca
     3481 ggcggcgggc aacccacggc cacgcacgcc ggaggacttc accagggagg agacttacaa
     3541 gcgtacgcgc ctgccggttg acctggcctc gaccctgttg ccggaggctt acacttcgga
     3601 ggagttcttc gcgatcgagc gggagaggat cttcgcctcg ggctggatcg ccgtgggctg
     3661 cacctcccag ttggacgatc cgggaaagac gcttgtggcc agggtggcgg ggcgctcgat
     3721 catcgtcacc cgcgataagg ccggcgtgct aagagccttc cacaacgtct gccgccacag
     3781 ggcggcaaag ctcctggacg atggtcgcca gatgatccgg aacaacagga tccgctgccc
     3841 ctaccactcc tggacctacg acctcgaagg caagtgcctc ggaactccgc tcttcgaggg
     3901 ctccgatata cccaaggaac agcaggccat cttcgacatg tcggatgtca aaggcttcga
     3961 caaggcggac tatggcctca tgccggtaag ggtagatagc tggggcttcc tggtcttcat
     4021 caacctcgat cccgacgccg cgccactcga ggagttcctc ggcgacctgc cggaacgctg
     4081 ttcgggttac cggctcgacg agtggcaggt ggtccgggag aagcgctacg agatagcgtc
     4141 aaactacaag ctcatcggcg agaacttcat ggagttctac cacctgccgt gggtgcatcc
     4201 tgagctggtc gaggtctcca ggatggagga ccactaccgt tggcaggggc cggggatgta
     4261 catgggcttc tgcacgactc ccatctccca gaacaccgac gccggcggct ggcagggtct
     4321 gcctccggtc ggctccttga gcgggacgga cgccgagagc gggcggttca tctggctctt
     4381 ccccaacacc gccgtcaaca tcctgcccaa ccactccttc atcatgatga cccgccctga
     4441 gggcccgtcc cgcaccatcg aagataccta catcctcgcc caccctgagt ccgccgatac
     4501 tccggactcc gaagaggcgc tcgaccagtt ggcgaagttc tggaacctgg tgaacgtcca
     4561 ggacgtggag attgtggagc gggtgcagga ggggatctcg aacccggcct acaggggtgg
     4621 gaggatgtgc tatcgcttcg aagagccgtt gcaccgctac cagaacatga tcatagacaa
     4681 gatggtgggg gtggatcggg tgccaccggg cgacgaggag cagatgagcc agatgttcgt
     4741 gaatccttcg gagacccccg actggacgag cccggagtaa aagcaagccc gagaccccga
     4801 ttggacgagc ccacagtaga ggcaagttcg attagaggaa gccacaacaa gttattgaac
     4861 acgagattct tacggaggta gacaggatgc agctaccgga gcggagcaag tacgtgatca
     4921 tcggcgccgg ggtacacggc ctgagcacgg cctggcacct ggcgaaagag ctgaaggctc
     4981 ggggccgtgg ctcgggcgaa gacgtgatcg ttctggacaa gaccggcatc gccgcaggag
     5041 cctcggggat cgcatgtggg acggtccgca acaactactt ccagcccgcg atgcgcgagc
     5101 taatggctta ttgcgtctcc gtctgggaat cggaccctga agcgtttgcc taccatccgg
     5161 tgggatacat gcagatttca ccggaggtca tgcacgagga catcgtccag atctacaagg
     5221 agcagcaggc aattggctat ccatccaccc tcgtggaggg ggaagatgaa tgccgccgct
     5281 acatgaagaa tctcttccac gactggcggg cggagaacat caccagcgtc ctgcacgagc
     5341 atcgcggcgg ctacgcccac aacaaagctt cggtaagagg tctcgccgaa aaagcccagg
     5401 ccgagggagt acggatccat accggtgtcc gggtcacagg cctgcgcctc gagggcggcg
     5461 ccgttgaagc cgtggagacc gaggagggag cgatccattg tgaacagcta gtcgtaggcg
     5521 tgggtccctg gatccgggac gtctgggcga tgctcgactt acccgagaag atcacgatca
     5581 tggattccga aggcaggctt caccgcgacc gcgatatgtg gacgtactgg tcgctgcagg
     5641 agggtacgct gggcgtggac ccgaacttcc tcacggacaa tgacggcgcc atgccgccgg
     5701 tggtccacgt ggataccgac gccccactct acgacgatga gggagagctc gtcacgaacg
     5761 ggctgtgggg catctattac aagccggact tcaacttcgg gggcgtgcag ggcggggccg
     5821 cgccacacgt ggttgaccag ccggcacagg aggtggccgt agacccttac ggaccagaga
     5881 gtccggactt cgtggtcgga gaagacttcg ccaggatgtg gaccgcggcg ctctcgcact
     5941 gccaggcgcg cttcgagggc aagcgagggc tcttctccga agaaccgtcc gggggcatcg
     6001 gctgcttcac gccagacagc ttcccggtct tcgacacctt ccgccaaaac gcctacgtca
     6061 tcgccgactc caaccacggc tacaagatga tcggcgtcgg tgcgctcgta gcgaaggagc
     6121 tgatggatga gccccaaagc ctgttggagc cgttccgatt ttcccgctac gagaccggca
     6181 agctgcatcc gacgagccac tctccattcc cctggagcta gaaagagccc actatgccag
     6241 agagccgcga ccagttattc atcgccaacg ggtggactcc cgcccgcgac ggtcgtactt
     6301 tcgagaccat agacccctct acggaagagg tcctcgccac cgtcgcacgc ggcggcacgg
     6361 aagacgtaga cgacgcggtc gccgccgccc gcgcggcttt cgacgggccg tggcgtcggg
     6421 tgacaccgca ggagcgtgga cgactgttgt accgggtggc gcgggagctg gaggccagga
     6481 ccgacgagtt cgccgagctg gagaccctgg atacgggcaa gccgctcggg cacgcgcgcg
     6541 gtgagatctc aggctgcgtc ggctacctcg actactatgc gggtatggcc gacaagatcc
     6601 acggcgagac aataccgctt ggcccggact acctcaacta cacggtgcgc gagccgctag
     6661 gcgtgacggc ccacatagtg ccgtggaaca tgcccctcag catggtctgc cgcagcctcg
     6721 ccccggctct cgccgccgga aacaccgccg tcataaagcc tgccgaacaa actcccctga
     6781 ccgccctgaa gttcgccgag atattcttgg agcttggttt cccgccgggg gtctacaatg
     6841 tcgttactgg cttcgggggc gaagccggca aggcgctcag cgaacatccc ggtataggtt
     6901 cgatcacctt caccggctcg gtggagaccg gacgtagcat tctgcatgcg gccgccgagc
     6961 acgtgaagcc ggtcgtgctg gagctcggcg gcaagtctcc acaggtcatc ttcgccgaca
     7021 ccgacctgga cctcgcggct gcagaggttg ccaaaggcat ctactccaat accggccagt
     7081 actgcgacgc gggctcccgt ctcctcgtcg aagagggagt cagagagcca ctcttggaga
     7141 ggatgctgga gcactcccgc cagatagagc tcgggatggg catggacgac cccgacatgg
     7201 ggccgctggt ctccgccgag catctggagc gcgttctgag atacatcgcg tcgggccagc
     7261 aagagggcgc caacatgctc atcggtggga gggccgagga gttcgagcgg ggatacttcg
     7321 tctccccgac cgtcttcgat ggggtaggga gcaagatgcg gatagcccag gaggagatct
     7381 tcggccccgt tctctcggtc ttcccctttt cggacgagga cgaggccgca gagatggcca
     7441 acgactcgag ctacggtctc gccgccggga tcttcacgcg ggacatggac cgcgccctgc
     7501 gcttcgcaag ggacatcgag gccggatacg tcatgatcaa cgagtacttc tctggtggcg
     7561 tgggctctcc ctttggaggc tacaaacaga gcggcatcgg gcgggagaag gggctggtcg
     7621 cactagataa ttacacccag atcaagaacg tggtagcgcg agtgagataa gccggacccc
     7681 gcactatggc tgacagtcgg catgccgatg atgggaaggt tcgaatgagt gatggcagcg
     7741 aaactgcgac cgtggtgagc aacctccctg gcatcgatga gaaatccgtt cgagcgtacc
     7801 gactcgaacg agtcatggcg cggttggttc gccacgatct cgcgggcatc gtgctgttcg
     7861 accctatcaa cgttcgttac gctaccggca gccggaacat gcaggtatgg gccatgcaca
     7921 acgcgatgcg gtacgcgttc atcgccccgg atggaccggt catcctcttc gattatccag
     7981 ggagctcgca cctttcggac cacctcgaaa ccatagatga gcaacgtccg gcggtagggt
     8041 gggactttta cggcagcggc gcgagaagcg aggaacacgc cgaaagatgg gccgccgaga
     8101 tcgccgacct ggtggtccgg cacggcggcg aaaacaaacg cctcgccatg gatcgcgcgg
     8161 acctgctccc cttgcgggcg ctcatgtccc gcggtgtgga cgtcgtcgac ggcaaggggg
     8221 tgatggagat cgcccgttcc gtcaagtccg atgaggagat ccgggccatg aggcattcca
     8281 tcgacatctg cgccaggagt atgacccgta tgcgcgaggt gctgaatccc ggcgtgcggg
     8341 agtcggagat gctggcgacc ttgatgtacg agaacgctcg tctgggggga gagtacccgg
     8401 agacgcgcct gctgaccagc ggcccgcgca ccaacccctg gcttcaagag acttcggatc
     8461 ggaccctcga gccgggcgat ctggtggcct tcgacaccga tctcatcgga tcgttcggct
     8521 tcttcaccga cgtgtcgcgc acgtggctgg tgggcgaggg caaacccagc gatgaacaac
     8581 gtcgcttgta tacgctggcg cgagagcagc tcgaacacaa cgcagagctg ctgaaaccgg
     8641 ggctgggttt tctggagatg accgaaaagt cgtggaagat gccggatgtc tacgtcccca
     8701 atcgttacgc cgagatcgtc cacggtgccg gtatggcggt cgaatacccg ctggtcttct
     8761 acccggaaga cgtggaggcg tccggctacg atggggtgtt cgaggagaat atggtggtct
     8821 gtgtagagag ctacatcggc gcggttggcg gacgcgaggg cgtcaaactc gagcagccgg
     8881 tcgtgatcac ccgtgacgga tcgcagccac tttgcactta tccttttgag gatcacttcc
     8941 attaatgagg tgaggtacag accccctggt gacagcggca gagggtatca acgctccgag
     9001 accatggcgg ccgactagcc ggaaggcgat gtgctcgggt ccgtgggtcg ggcgttaggc
     9061 gcagaagcaa actgtaggag gtcaggttat cgtgagagcc gtgctgctca ccggccacgg
     9121 aggaccggag aagctcgaat atcgggagga cgtacccatc cccgaacccg atcggggcga
     9181 ggtcctgatc gaggtcggcg ccgccgggat aaacaacacg gacatctgga cccgggaggg
     9241 agcctacggg gcggaggacg atccagataa ggtacagggc tggcgcaggg agcccctgaa
     9301 gttcccgagg atacagggag cggacatcgc cggccggatc gtcgccgtgg gtaatggggt
     9361 gtccgagggt aggatcgacg agagggtcgt cgtggacaac gccctctact tcggagatga
     9421 gaaagaactt ctcgactgcg ggatcgtggg tagcgagcgc gacggaggct acgcggagta
     9481 cgtcacggtc ccggccgaga acgcccacgc ggtattcagt gagctctcgg acgccgaact
     9541 ggctaccttc ccgacctcct acatcacctc actcaggatg ctgaaccgcg tcaggatcgc
     9601 cgaaggagag accatcctcg tgaccggcgc ttccggcggg gtagggaccg ggctggtgca
     9661 gctcgccaag ctgcgagggg cgtacgtgat cgcccaggtg ggatcgggca aggaagagca
     9721 ggcgcgcagg ataggggcgg atgcggtcgt acgtagggac gcgatggacc tcgccgccga
     9781 ggtgagggag gccgcggatg gaagacccgt cgatgtcgtc gcggacgtcg tgggcggccc
     9841 cgctttcgca aagctgttga acgtgctcag gccgatggga cggtacgtgg ccgccggcgc
     9901 gatagccgga ccggtggtaa gcctcgacct gaggacgctc tacctcaaac aattggagct
     9961 gatcggctcg accatgggga cccacgaaga gttcgccgag ctcgtcaacc atatctcctc
    10021 cgggcaactc gaaccgctgt tggcgaagac ctacccccta tcgcaggtcc gtcaggccca
    10081 ggaggatttc aaggccaagt cgttctttgg caagctcgcg ttgatccctt cagaatgatt
    10141 ccgagggcgc tggagcaaac ggcaggtaac tcgcgatcgg caacgatgtc gatgatgcgc
    10201 ccatgtccgt gccggcgatc tcgacgcacc ggtaccctca gacgccggtg atcctggggt
    10261 ccaggttaag gtgggccgat caagatttta ggaaggtgac cggattgtga tagacggcgg
    10321 aggaatgctg ggagcaccgc tcctcttcgc tgtagaactg agcatcctat acgctgccgg
    10381 tacctacgcg atgggccacc tgatggcatt ttcgggtttc ggtggtggcg gtaatgccct
    10441 gggacgtttg gccttctacc tcctggtgtt tccgggtgtg gtcctgcacg aaggtgccca
    10501 ctacctggca tgcgtcctca ccggaaccaa ggtcacccgc ttcgcaccat tctcgccggg
    10561 tagatcgaac gatggtcgtc tcctgttggg ctacgtccgt cacgaacgtc gcgtcttccc
    10621 tgtgggagcg attatcgggt tggcgccgat actcctgaac cctctgggac tcctgctcgt
    10681 caccgcgttg ctcacgcctc tcacacttca agaggtagcc aagccctctg ccgagggcgt
    10741 ggtagagggg ctcttcgtga gtggctttct aaccgacacc ccactgttgg ccactatctg
    10801 ggcgtacctg actttgagct tcgcgttggg aagcgtaccc agccgagaag acctgtcgag
    10861 cctgccgctg ctgctagtgg tcttcggagt cgggatcttc tcgatcagtc ttctaaggac
    10921 aggctccgag agagttctct ccccagcgat ccacgacctg gccgcgctgg cggccggact
    10981 ctacgccctg ccagccatag tagccattgt cgctgccctc acgatgggac ttgcaggtcg
    11041 gacgctacgg taatgaagcc gggtcgaggt cgctcatcgg tcctgctttg cctaggtgtg
    11101 caggtaagac gcgggagtat tgcctggtac gtaatgtacc tatcggagat gacggaagat
    11161 agcgccgcaa tccgggcact tacgctcccg aacactggga tgccgtgcac gcaagttcgt
    11221 tggtgccgaa cttcctcagg ccaggagcag gtctgccacc gtgtccacga cggcgagcag
    11281 cccgtccagc gtgaggttag tgacctcaga gcaccttccc tggaggaaga tctttgccgg
    11341 tcgttcgggt tcgttcggca actcgatcca ggccagggta ccttccgtgt gctggacgtc
    11401 ggatctgctg cagattatca ggtctgcgcg cggcgccatc atctctagct ccgcgagcgc
    11461 cgcgttcacg acttcggtgt gggggcggag cgtccgaatg gccgcatcta tcgtctccct
    11521 gtagctacgg tattcgtcct cataggccac cagcacccgg agacgcggtg gatggtcggc
    11581 gggctgttcc gttcgtctct cctcgccggc ggaggaggta ggcggtgcat cggcgcggca
    11641 catgctagga ccgacgatca tcgatttgct ccttccgtgg ccctcaggta ccccggagcc
    11701 ggcaggtcga tcccaggccg tagagggcgg acctccacgg gccaacgtgg gggagtcggg
    11761 tctataggtt cgctacccca ggtgatcata gtccatcttt cgtcggtgtc gcttggggcc
    11821 ttgcgccagt tagtctggac gaatagtctg ttattggcct gtcgaaatga tgaaaaaacc
    11881 gacaggaaag ccttgccggt ccgcacctta ggctaggata tagcttgaga tgatacgtat
    11941 tctgatcgtc gaggacgaag tatatttggc acggatgatc gccagagtgc tcggcgagga
    12001 gggatacgcg gccgaaacgg ccgagaccgg tagttcgggc ctcacgcgcg ggctctccga
    12061 acggttcgat ctcctgatag tcgactggat gctgccagat ctggacggga tccaggtggt
    12121 gcgagggctg cgctccgccg aggtgaacgt gccgatcttg atgcttactg ccagaaccca
    12181 gatagaggac cgtgtgagcg gcctcgacgc cggggccgac gattaccttt ccaagccgtt
    12241 cgccttcccc gagttactgg cacgcgtgcg ggcgcttacg aggcgcccgc acacgccccc
    12301 aggaatggca gtggtcaagt ccggtgacat agtgctcgac cccgtcaggc acatggtgtg
    12361 gcgcgggcag gagcacatag atctcaccgc caaggagttc gcgctcctcg ccaccctcat
    12421 ccagcggcct ggccaggtct tcacgcgctc gttgctgctc gccactatct gggccggcac
    12481 ccgagatgtg tataccaacg tcgtcgacct ctacgtctcg cacctacgga agaagctcga
    12541 ccgcgccggc gagccctccc acatacgaac cgtgcgcggg atcggctacg catacgagtc
    12601 gaggggtgga gccccctgat gttcggccgc atccggctca ggctgacgct ctggtacgtt
    12661 tctattctga tccttatagt agtgctcttc gggaccatcg tagtgctcgg tttccgctat
    12721 caggtgacgc gcgaacagga cagactgctc gcccgcgagg cggagagcaa gcaggggatc
    12781 gtctacggca acgagcccgg cggcgcaaat acgtactcca ccaactccaa ccagatcggg
    12841 tggacggtgc tagagccgga cggccgactc cttgcacaga ccaatatggg attatctctc
    12901 ggccttcccg accccgctct gcttaaggaa acgaccaggg agcggaagac cagtaaggcg
    12961 accgtgaccg agtctggggg aggcgtaagg gtcctgagcg tgccggttac ccgatcaggg
    13021 gaagtgatcg gcgtagtcca ggtggcgcag tcgcacgagt tcgtcgagaa gaccgtggac
    13081 aacttcattg ttcggctggt gcccatcggt cttctggcgg tcggcctcgc cgccgtcgga
    13141 gggcttttcg tttctggaag ggcgatgagg cccgtgcgcg acgccttcga aaggcagcaa
    13201 gcgttcgtgg cagacgcctc ccacgagctg aaggcgccgc tcaccctgat cagggtagac
    13261 gccgaagtga tgggccgccg tcctagggat cgcgaagacc aggaaatctt gcagcacttg
    13321 ctcggggaga cgacgaggat ggacgcgatt ctctccgacc ttctattgct cgcgcggctc
    13381 gacgccggcg aaacgccgac ggtacgcgag acgttcgatc tgaccactat tctcaccgag
    13441 gcggccgacc gcttcgagac cgaggcggcc gtcgagggca tcggcctggt cgtcgacgtc
    13501 gagggaaagc tcaacgccac aggcgacaga gaatatacgc gacagatact cggcgcgctg
    13561 ctcgacaacg ccctgagata cacacccacg gggggcagcg tcaccgtaga aggtcgctcg
    13621 gaagacggta ggatcgaggc gaccgtgcgg gactccgggc ccgggatccc gccggagcac
    13681 cttccgcacg tcttcgaccg tttctaccgc gtcgatgccg cccgcacgag gaaagggggc
    13741 gggacgggtc tcggtctcgc catcgcccgc ggtctcgccc gtgcccatgg cggtgatctc
    13801 acggccgcca acgcacacgg cgggggcgcg atcttcaacc tgagcctgcc ggctgcgcca
    13861 cgccactgat agccggaaat ggctagactg agagggctag aaaaggacca tcgattatgt
    13921 cgacggcagg ttcgcccatc cgggctatac tctaggtggg acagaatgcc gccacgcaag
    13981 aaggtgatcc cagtgaaggc gaatgcgtcg gcggtcttca cctggcgtgc agaccgcaag
    14041 agtacgatcg gacgtgcaat tcagtggcgc gccgtttcta gattcgccca ccccgcgatc
    14101 agggacgatg ggcaccgcct tatcaagggc gcagccactt tgttggttgc cctgctcgta
    14161 tctacatcgc tatggtcgat cctggtcgtc ttgccggccg gggccgagct ctccgagacg
    14221 gggcccgtag atcctgagac ggggttccca gtctggctcg aggacgggaa agggttgcgc
    14281 ctggaacctt gctttaccta cccgaactgt cctcaggatg gtagaggatc atcctctgtg
    14341 gcttcttacc aggggggcga tgtcgtttac tggtcagcaa cgtcgaagat atcgaacgac
    14401 ggcgccgaag cgctgctggc catgagcacc cggggtgact tccgggaagg cccggtgctc
    14461 accgagcagc gggtctacaa ccggatcagg atccgggccg acggcctcgt acccggcgcg
    14521 acgtacaggg ttacttatcc gtacgggacg gagacgttta ccaacgtggc cggagggcag
    14581 cgaggtatcg actttatgga ggacataggt tgcctgcagg ccccgtgcgg cggcttcaca
    14641 tcggccctga acggccgcgt gggcccctgg ctgacgtgga ccacgctcgc ctcatccgac
    14701 gatgacctgc ccgccgggta tatcggtgat ccccacaagc ttcatgaggt gatcggcagc
    14761 ccggttctgg actcgggcag cgaaccacag aactacttca agatcgaagg ccccgatgtc
    14821 ggggggcctg gggacgacgt ggttcgcaac gatctgttct ggctcggcgg caaggtccac
    14881 ggtctcgccg tgtcggcgaa tccgaagggc ggggtttacg actcgggctc gtccgtgacg
    14941 ttgacgtcct cagatcctgc ggcgaaaatc ttctacacca cagacggtac cgagccctcc
    15001 gcggacagca cgccgtatga gggcccgatc caagtagagg cgccgacgac actcaagtat
    15061 atggcactcg gaccggccga cgaagcgggt aaccggcccc gctcaccggt ctcgaccgaa
    15121 acctatgagt aaggcgcccg actctcggac gtagccgcgg gtcaaccctc cggccccgcg
    15181 gctgttcgct cccgtccagc atgccgaaac aataacgccg atccgacgaa cag
//
LOCUS       DATMVF010000123         9040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_609494, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000123 DATMVF010000000
VERSION     DATMVF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609494"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VN178_06080"
     CDS             <1..904
                     /locus_tag="VN178_06080"
                     /inference="COORDINATES: protein motif:HMM:NF044298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HWS80575.1"
                     /translation="WNWRMLGIEGDARFADLIEHVLYNAVMPGISLDGQHYFYQNPLA
                     DDGTHRRQPWFGCACCPPNVARLLASLPGYFYSTSEGAVWVHLYAAGAATVELDENRT
                     ISLRQRTRYPWDGRVEIDVEAEGELTLMLRIPSWCEEGPQIEVNGDPVDVEIFPGSYV
                     NLRRMWRSGDTINMDLLMPVRRVECHPYVTENTGRIALMRGPLLYCAEQTDNPGVDLR
                     DLLLNGEEPTVSLEPDLLGGVVVLQAGARSAAPGPEWEDRLYRTVRSHEGEAQTNSTR
                     VTAVPYYVWANREPGTMRVWLKNR"
     gene            1087..2070
                     /gene="galT"
                     /locus_tag="VN178_06085"
     CDS             1087..2070
                     /gene="galT"
                     /locus_tag="VN178_06085"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HWS80576.1"
                     /translation="MNQLRWDPTLREWVAYATHRQDRTFLPPAEYCPLCPTKPGGFPT
                     EVPREHYDIVVFENRFPSFAPDASEPDEGGSDLTPTAPGRGICEVVLYSDDHDATLAT
                     MSERRIRNLIDVWADRYKELGASEEVDYVFIFENKGEAIGVTLHHPHGQIYGYPFIPP
                     RPAKELEAAIEYRAENEGRCLHCDLLSQEHEDARRVLIKGEHFTAFIPFYAHFPFEAH
                     VYSHRCAPSIADLESAERRDLASVLKRLLVGYDSLFGFSLPYMMVMHQAPTEGGAYGG
                     IAHFHIEFYPPNRTADKLKYLAGSETGAGAFIMDVLPEVSAETLRGKIEGS"
     gene            2067..3038
                     /gene="galE"
                     /locus_tag="VN178_06090"
     CDS             2067..3038
                     /gene="galE"
                     /locus_tag="VN178_06090"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWS80577.1"
                     /translation="MKVLVTGGAGYIGSVVASQLVDAGHETVVLDDFSKGHREAVPEG
                     TRLLQGNLMDAGFVRETLSEGFDGVLHFAALSLVGESVEQPEIYYRNNVCGTLNLLEA
                     MREAGTGRLVFSSTAAVYGEPEEVPIQETAPALPTNPYGSSKLAVDRLIGAVCGARGL
                     AATSLRYFNVAGASSRFGEDHHPETHLIPLVLRAAAEGSSVKIFGTDYPTRDGTAVRD
                     YIHVEDLGRAHLLALEAAEPGEHGVYNLGNGTGFSVKEVVDAARRVTGKIIDAVEDPR
                     RAGDPPTLVASSEKIQAELGWRPEKPGLQTMISDAWTWMQNHPNGYE"
     gene            3048..4175
                     /gene="galK"
                     /locus_tag="VN178_06095"
     CDS             3048..4175
                     /gene="galK"
                     /locus_tag="VN178_06095"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HWS80578.1"
                     /translation="MRETEQRAARAYKEQFGGDPELVASAPGRVNLIGEHTDYNGGFV
                     LPCAIDRRVAVALGTDHRDGFETRLYSADYDETRQMDDKDSSWADYPRGVVWAMGEAG
                     HEIGAFRAAFAGNVPLGSGLSSSAAIEAATALALNAFFGLGVGRTDLAVICQRAENDY
                     VGVGTGIMDQYASLLCEAGAALLVDCRSLDAERVPLDLEAAGLALVVCDTRVERGLAD
                     TGYNDRRATCERAAKMLGVEELRDATEEDLNLLSGAELMRARHVISENARVLEAVDAL
                     TDKDFEEFGRLMYASHASLRDDYEVSTPELDTFVEAAEQHGARGARLTGAGFGGCAIA
                     LVPEDETAALTKACERAFASSRFEEPAFYEFVPAAGAEIAD"
     gene            4327..5340
                     /locus_tag="VN178_06100"
     CDS             4327..5340
                     /locus_tag="VN178_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HWS80579.1"
                     /translation="MSRETRDRSLRELMDRHGVGALLLSSPANFAWYTGGADNRVDHG
                     DPDGVASVLLTPDSACILTNNIEAPRMREEQTPEMELVEHSWHEEPSTLLRDLTGGAG
                     VGTDFPSTSGPDLSAEISPLRYVLDEDAIKDYRRLGEDTVQAMSETADSLSSETDEFE
                     AAAELSSACLRRGMYAPVLLAATGERLARHRHPVPHGGPLGKQAMLVVCAERGGLFAS
                     LTRMVYFQDPDPQTAGRQEACEDILRRMRGEATREGSTLAQAFEDCRRFYAEAGFPEG
                     WRDHHQGGMTGYASREIIATPETRQEIRQGQAFAWNPSLVGAKAEETFVLGQDGPEIL
                     TRS"
     gene            5440..6015
                     /locus_tag="VN178_06105"
     CDS             5440..6015
                     /locus_tag="VN178_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWS80580.1"
                     /translation="MANELQGKKIAILLAPVGTEQVEFEEPKKAVESEGATVDVVGLQ
                     TGDAQTMNSDVNPGDTFTVEKTFSEVSADEYDGLVIPGGTVGADNLRGSDEAVSFVHS
                     FFEQAKPVGAICHGPWKLVEADVVKGRTLTSYPTLQTDIRNAGGNWVDEEVVVDQGLV
                     TSRNPDDLEAFCAKIVEEFAEGKHEDQRRSA"
     gene            6167..6775
                     /locus_tag="VN178_06110"
     CDS             6167..6775
                     /locus_tag="VN178_06110"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWS80581.1"
                     /translation="MPQRVESSVEIEAPVEKVYDYWKTLENLPQFMTNVEEIRSTGPD
                     TTHWVVKGPLGAKLEFDAKTTQNKPNEAIGWNTENGQVQTSGQVRFQKVNEDRTRVEV
                     QMNYWDAPGGKAGEVASRIVANPELMVQQDLQNLKDILEGTATPEDIQQRPAVATAQS
                     SAVAFLTSGTGLLLVLGGGLVLWLLLRRRSSSRRKSRIIFEF"
     gene            complement(6879..7139)
                     /locus_tag="VN178_06115"
     CDS             complement(6879..7139)
                     /locus_tag="VN178_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80582.1"
                     /translation="MAEQHTEQSIMVRQVTDVHANWSEQERGAPGKFSFQLILDSGAE
                     EYAIRPPAEDADVLVELFTLTDKIYFDMSRQVLIFGDVPIGS"
     gene            complement(7170..7427)
                     /locus_tag="VN178_06120"
     CDS             complement(7170..7427)
                     /locus_tag="VN178_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80583.1"
                     /translation="MAEQHTEQTIRVRQVTDVQVSWTERGRGEQGAFTVQLILDSGAE
                     EYVLQPTAEDTKVMVELFKRANAVYFDLDRKVLVPNNIPLG"
     gene            complement(7510..7974)
                     /locus_tag="VN178_06125"
     CDS             complement(7510..7974)
                     /locus_tag="VN178_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWS80584.1"
                     /translation="MDKETGLLKVDRILYSAVIYPANYGFISRTLGDDGDPLDVCVLM
                     QEPVQPLSLLSARPIGMMNMVDEGESDEKIICVHPDDPEYRSYEHHGELPEHSLAELQ
                     RFYQDYKVLEGKEVDVGEFYDPQAAKDAVRHAIRLYDEHFVQTSQASGAKAE"
     gene            8368..8754
                     /locus_tag="VN178_06130"
     CDS             8368..8754
                     /locus_tag="VN178_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS80585.1"
                     /translation="MEINKDLIAASSTPIVLAILAEEDSYGYAILKRVRELSGGRMEW
                     TDGMLYPVLHRLERLGHVEARWEVAESGRRRKYYRITSQGRAQLGEERRQWQAVDDTL
                     RGVWRALSLSVPASHPAAAAPLPQGA"
     gene            8758..>9040
                     /locus_tag="VN178_06135"
     CDS             8758..>9040
                     /locus_tag="VN178_06135"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HWS80586.1"
                     /translation="MPAPGDAVSLEEQIDRWRSYLRHRQAIHSVDVAELEDHLREQVA
                     VLVDAGLATDEAFLVAVKRMGKLDDLSREFAREHSDRLWKQLVVVPSDFG"
BASE COUNT         1786 a         2807 c         2823 g         1624 t
ORIGIN      
        1 gtggaactgg cgcatgctcg ggatcgaagg cgacgccagg tttgcggacc tgatcgaaca
       61 cgtcctctac aacgctgtca tgccgggcat atccctcgac ggccagcact acttctacca
      121 gaacccccta gccgatgacg gcacccaccg ccgccagccc tggttcggct gcgcctgctg
      181 cccacccaac gtggcccgcc ttctggcctc actccccggc tacttctaca gtacttccga
      241 aggcgccgtc tgggtgcacc tctacgcggc gggcgcagcg acagtcgaac ttgacgagaa
      301 ccggaccatc agcctccgcc agcgcacgcg ctacccgtgg gatggaaggg tggagatcga
      361 cgtcgaagcc gaaggcgagt taaccctgat gctccgcatt ccatcctggt gcgaggaagg
      421 ccctcaaata gaggtgaacg gtgacccggt agacgtggag atcttccccg gttcatacgt
      481 gaaccttcgc cgcatgtggc gttctggcga cacgataaac atggacctac ttatgcccgt
      541 tcgtcgtgtc gaatgccacc cttacgtcac cgagaacact ggtcgtattg cgctcatgcg
      601 cggtcccctg ctctattgtg ccgagcagac agataacccc ggtgtcgacc tacgcgacct
      661 cctcctgaac ggcgaggagc cgacagtcag tttggagcca gacctactcg gaggcgtggt
      721 cgtactgcag gctggggctc ggtccgcagc gcccggccca gagtgggaag accgtctcta
      781 tcgcaccgta cgttctcacg aaggggaggc gcagacgaat tccaccaggg taacggccgt
      841 tccctactat gtatgggcca accgcgagcc gggtacgatg cgcgtctggc tcaagaacag
      901 atgacctgag ccgcgcaatg tgtgccttaa ctggagagcc cttgcgatgg ccgctggcag
      961 taaggtgatg gtatcgtatc cggtggaccg ggaggactaa gaaatcgggt tggaaaaaga
     1021 gatcgagcag aaggacgacg gccgctacat catcttctac accttcgatg acgaagagga
     1081 agacgattga accagctccg ctgggacccg acgctgcgcg aatgggtggc ctacgcgacc
     1141 caccgacaag accgtacttt cctgcctccg gcagagtact gtccgctgtg cccgacgaag
     1201 cccggcggct tcccgactga ggtgcccagg gagcactatg acatagtcgt cttcgagaac
     1261 agattcccct ccttcgcccc agatgcctct gaacccgacg agggcggcag cgatctgacg
     1321 ccgacggccc cagggcgcgg aatctgcgag gtagtcctct attcggatga ccacgatgct
     1381 acgcttgcga ccatgagcga gcgccgcata aggaacctca tcgatgtctg ggccgaccgc
     1441 tacaaagagc tcggcgccag cgaagaggtc gattacgtct tcatcttcga aaacaagggc
     1501 gaggctatag gagtgaccct gcaccacccg cacggccaga tctacggcta cccattcatc
     1561 ccaccgcgtc ccgcgaaaga gctggaggct gccatagagt acagagcgga aaacgaaggc
     1621 cgctgcctgc actgcgatct gctctcccag gagcacgagg acgcccgcag ggtactgatc
     1681 aaaggcgagc acttcacggc cttcatccca ttctacgccc atttcccttt cgaggctcac
     1741 gtctattcgc atcgttgcgc gccctccata gcggatctcg aaagcgcgga acgccgcgac
     1801 ctggcgtcag tactcaagcg gctgctcgtc ggctacgata gcctcttcgg tttctcgctc
     1861 ccctacatga tggtcatgca ccaggcgccc acggaaggcg gagcctacgg tggcatcgcc
     1921 cacttccaca tcgagttcta cccgccgaac cgtaccgcgg acaagctgaa gtacctcgcc
     1981 ggcagcgaga ccggggccgg cgcgttcatc atggacgttc tgcccgaagt gagcgccgag
     2041 accttgcgcg ggaagatcga aggctcgtga aggtacttgt gacaggcgga gccggttaca
     2101 tcggtagcgt cgtcgcttcc cagctcgtcg atgcgggcca cgagacggtc gtgctcgacg
     2161 acttctccaa aggacaccgt gaggccgtac ccgaagggac gcgacttctg caagggaacc
     2221 tcatggacgc cggtttcgtc cgcgagacgc tctccgaagg cttcgacggc gtgctccact
     2281 tcgcggcgct ctccctcgtc ggcgagtcgg tagagcagcc ggagatctac taccgcaaca
     2341 acgtctgcgg aacgctcaac cttctggagg cgatgcgcga ggcgggtaca gggcggctcg
     2401 tcttttcgtc gacggccgcc gtctacgggg agccggagga ggtgcccata caagagactg
     2461 ccccggccct gcccaccaac ccttacggca gctccaagct cgccgtagac cggctcatcg
     2521 gcgccgtgtg cggggctcgt gggctggcgg cgacgagcct tcgctacttc aacgtggctg
     2581 gggcgagcag ccggttcggg gaggatcacc atcccgagac gcacctgatc cctctcgttc
     2641 tcagggccgc ggcggagggc tcgtccgtga agatcttcgg caccgactac ccgacgcgtg
     2701 acggcacagc cgtacgcgac tacatccacg tcgaagacct cggccgcgcc catctgctgg
     2761 cactcgaggc ggccgaaccg ggagagcacg gcgtctacaa cctcggcaac ggaaccggct
     2821 tctcggtcaa ggaggtagtc gatgccgccc gccgcgtcac gggcaagatc atagacgccg
     2881 tcgaggaccc gcggcgggcg ggagacccgc ctacgctcgt ggcctcgagc gagaagatcc
     2941 aggccgaact cggttggaga ccagagaagc cagggcttca gaccatgatc tccgacgcct
     3001 ggacctggat gcagaaccat cccaacggtt acgagtagga gcctgccttg cgagagacag
     3061 aacagagagc ggcccgggca tacaaagaac aattcggcgg ggatcccgaa cttgtagcca
     3121 gcgcccccgg ccgcgtgaac ctcatagggg agcacaccga ctacaatggc ggattcgttc
     3181 tcccctgcgc catcgaccgc cgcgtcgccg tcgcattagg aaccgaccat agggacgggt
     3241 ttgaaacccg cctctactct gccgactacg acgagacacg ccagatggac gacaaagact
     3301 cttcctgggc cgattatccg cgcggcgtgg tgtgggcgat gggggaggct ggacacgaga
     3361 tcggggcgtt cagggcagcc ttcgccggga acgtgccgct cggctcggga ctctcctcat
     3421 ccgccgccat cgaggccgcc acggcgctcg ccctgaatgc tttcttcgga ctcggggtcg
     3481 gccgcaccga cctcgccgtc atctgccagc gggccgagaa cgactacgta ggggtgggta
     3541 ccggcatcat ggaccagtat gcctccctgc tctgcgaggc cggtgctgcc ctgctagtcg
     3601 actgccgttc gttggatgca gagcgtgtgc cgctcgacct cgaagcggcg ggtctggccc
     3661 ttgtcgtctg cgacacccgc gtggagcgcg gcctggccga cactggctac aacgacagga
     3721 gggcgacgtg cgagcgggcg gcgaagatgc ttggtgtcga agaattgcgc gacgccactg
     3781 aagaggacct gaatctcctc tccggagcgg agctgatgcg cgccaggcac gtcatctccg
     3841 agaacgcccg cgtcctagag gcggtcgatg cgctcacaga caaagacttc gaagagttcg
     3901 ggcgcctgat gtacgcctcg cacgcctctc tgcgcgatga ctatgaggtc tcgacccccg
     3961 agctcgacac cttcgtagaa gcggcggaac agcacggcgc caggggggcg cgtctcacgg
     4021 gcgctggttt cggcggatgc gcgatcgccc tcgtacccga agacgagacc gccgcgctaa
     4081 cgaaagcttg cgagcgggct ttcgccagca gccggttcga ggagcccgcg ttctacgagt
     4141 tcgtcccggc ggcaggggcg gagattgcag actagaccga tttgcaacag tatcgaccca
     4201 cggcgaatca cgcacctgcc tgagagcgat cggctactcc gaaaagagct gaaatgctga
     4261 agtgctgtca agctgccgca ggcagcgcgc tgacatgcta tggctatacc gttgggaggc
     4321 acgtagatga gcagagagac cagagatcgc agtttgcggg agctgatgga cagacacggc
     4381 gtaggggcgc ttctcctgag cagtccggcg aacttcgcct ggtacacggg cggcgcggat
     4441 aacagggtag accacggcga ccccgacggc gtggccagtg tcctgctcac cccggatagc
     4501 gcctgcatcc tgacgaacaa catcgaggca ccgaggatgc gcgaggagca gacgccggag
     4561 atggagttgg tagagcatag ctggcacgaa gagccctcca cgctcctgcg agatctgacc
     4621 ggcggcgccg gcgtggggac ggactttcct tctacgtctg gcccggatct gtcagccgag
     4681 atctcaccgc tgcgctacgt cctcgacgaa gacgcgatca aagactaccg gcgcctgggc
     4741 gaggacacag tccaggcaat gtccgagacc gcagactcct tatcttcaga aacggacgag
     4801 tttgaggccg cagcggagct atcgtccgct tgcctcagac gcggcatgta cgcacccgtt
     4861 ctccttgccg ccactgggga gcgcctcgca cgtcaccggc atcctgtccc acatggcggg
     4921 cccctgggaa agcaggctat gctcgtcgtc tgcgccgagc gcggcgggct cttcgcgagc
     4981 cttacgcgta tggtctactt ccaggaccca gacccgcaga cggccgggcg ccaggaggcc
     5041 tgcgaggata tcctgcgtcg catgaggggg gaggctaccc gcgagggaag taccttggcc
     5101 caggccttcg aggattgccg ccgcttctac gccgaggccg gcttccccga agggtggagg
     5161 gatcaccacc agggagggat gactggatat gcgtcgcggg agatcatcgc gacgccagaa
     5221 acacggcagg agataagaca gggccaggct ttcgcttgga acccctcgct cgtaggcgcc
     5281 aaggccgagg agactttcgt tcttggtcag gacggtcccg agattttgac ccgctcgtaa
     5341 caagtttgta aagctttctt aagaaacgat ctcgaccgtt tggtcgctgc tttcgtcggg
     5401 tatcccaaag ggtgtaagtc catcgaaagg agcatcccag tggccaacga gttacagggc
     5461 aagaagatag ccatcctcct ggctccggta ggcaccgagc aggtcgaatt cgaggagccg
     5521 aagaaggcgg tagagagcga aggcgccacg gtcgacgtcg tgggcctcca gacgggagac
     5581 gcccagacca tgaacagcga cgtcaaccct ggtgacacgt tcacggtaga gaagacgttt
     5641 tcggaggtct ccgccgacga gtatgacggt ctggtcattc ccggtggcac tgtgggggct
     5701 gacaatctgc gtggcagtga cgaagccgtg agcttcgtgc attccttctt cgagcaggcc
     5761 aagccggtcg gcgcgatctg ccacggacca tggaagctcg tcgaagccga cgtggtgaag
     5821 ggacgtaccc tgacctcata ccccaccctc cagaccgaca tccgcaacgc tggcggcaat
     5881 tgggttgacg aagaggtcgt cgtcgaccag ggactcgtca cgagccgcaa ccccgacgac
     5941 ctggaggcgt tctgcgccaa gatcgtcgag gagttcgctg agggcaagca cgaagaccag
     6001 cgaagaagcg cttagaagaa cataggagct cgataacaca ccacttcgcc gggttccgtc
     6061 tcgaacggga cccggttttt gcgtaggtca gaggctggct ggtttactca tcggcgagag
     6121 gggtaagaag gccgcttgga tgactgtagt cggcacagga ggacagatgc cgcagcgtgt
     6181 ggaatcaagt gtggagatag aggctccggt cgagaaggtc tacgattact ggaagactct
     6241 ggagaatttg ccacaattta tgaccaacgt cgaagagatc cgatcaacag gtcccgacac
     6301 gacccactgg gtggtcaagg gtccgttggg ggccaagctc gagttcgatg caaagacgac
     6361 ccagaacaag cccaacgagg cgatagggtg gaacaccgag aacggccagg ttcagacttc
     6421 tgggcaggtg cgctttcaga aggtgaatga ggatcgtacc cgcgtcgagg tccagatgaa
     6481 ctactgggac gcgccaggtg gcaaggccgg tgaggttgcc tcaaggatcg tcgccaaccc
     6541 cgagctgatg gtgcagcagg acctgcaaaa tctcaaggac atcctggagg gcacggcgac
     6601 cccagaggac atccagcagc gtcccgcagt cgcgaccgcc cagagcagcg cagtagcgtt
     6661 cctgacctcg ggtacaggcc ttcttctggt gctcggcggc ggacttgttt tgtggctact
     6721 tctccgcagg cgcagctctt ctcgccgcaa gtcccgcatc atcttcgagt tctgaagacc
     6781 cgtaaacgga tcactggggc ctccatagag aggttctagc ctgttcggca cacaagacaa
     6841 agggcccggt cggggccctt tgtctttccg cggttccgct aacttcctat cggtacatcc
     6901 ccgaagatca ggacttgcct gctcatgtcg aagtagatct tgtcggtgag tgtgaagagt
     6961 tccaccagta catcggcgtc ctcggcaggg gggcggatcg catactcctc ggcgccgctg
     7021 tccaggatga gttggaacga gaacttgccg ggcgcgccgc gctcctgctc gctccagttc
     7081 gcgtgcacgt cagtgacctg acgaaccatg atgctttgct cggtgtgttg ttcagccact
     7141 ccagtcctcc tgtagttcct tcgggttgat taacccaggg ggatattatt ggggacaagt
     7201 accttccggt cgaggtcgaa atagacggcg ttggcccgct tgaagagctc gaccatgacc
     7261 ttggtgtcct cagcagtggg ctgcaaaaca tactcctcgg caccactgtc caggatcaac
     7321 tgtaccgtga aggcaccttg ctccccccga ccccgttcgg tccacgagac ctgcacgtcc
     7381 gtgacctggc ggaccctgat cgtctgctcg gtgtgctgct ccgccactta gtctcccttc
     7441 ttccgcgcga ttggcatctc tgtccgacgt tcttgcccgc accagaagga tctaaactta
     7501 ccagcccgct cactctgctt tcgcgcccga agcttgactc gtttgaacga agtgctcgtc
     7561 gtagaggcgt atcgcgtgcc tgacggcatc tttggccgcc tgtgggtcgt agaattcgcc
     7621 cacatccacc tccttgccct ccaacacctt gtagtcctgg tagaagcgtt ggagctccgc
     7681 cagactgtgc tctgggagct ctccgtggtg ttcgtaggag cggtactcgg ggtcgtcggg
     7741 gtggacgcag atgatcttct cgtcgctctc gccttcgtcg accatgttca tcatcccgat
     7801 cgggcgtgca ctcagcaagc taaggggctg gaccggttcc tgcatcaaga cgcacacgtc
     7861 gagtggatcg ccgtcgtccc ccagggttct tgagataaag ccgtagttgg ccgggtagat
     7921 cacggccgag tacaggattc ggtccacctt gagcaacccc gtctctttgt ccaaaactcg
     7981 tacttgaccc tggagccttt ggggatctcg atcagggtcg tgaacctctg cggggcttcg
     8041 tcaccgatcg gtacgtcgtg ccagggatgg gatatcttgg aggctcctct cgggaaaaca
     8101 gggccgaggc tcgtgccggc cacagaccta ctcggaatcc tctttaccgc cggacgtaag
     8161 caattatata ggaggcactg agtactgcct ttgctaggtt cagacagcgt ctctccattg
     8221 tggatgccaa gaaggagaac accacgcaca tcgatctcac ccccggcagt agatctaccg
     8281 gactgaagat tggtatacat agcactgcca tgtataatgc cgctatgtac accggagtta
     8341 gagttcgtta gcgcgacgcg tgaaatcatg gagatcaaca aggacctcat agccgcttcc
     8401 tcaacgccga tcgtcctagc gatcctggcc gaagaggaca gctacggcta cgccatcctc
     8461 aagcgagttc gggagttgtc cggggggcgc atggagtgga cggacgggat gctctacccc
     8521 gttctgcatc ggctcgagcg gctcggccac gtcgaagcgc ggtgggaagt tgcggagagc
     8581 ggccgccgtc gcaagtacta ccgaatcacg tcccagggcc gggctcagct cggcgaggaa
     8641 cgcaggcagt ggcaggcggt ggacgatacg ctgcggggcg tctggcgggc gctctccctt
     8701 tccgtcccgg ccagccaccc ggcggcagcc gcgccactgc cacagggagc ctgagcgatg
     8761 ccggctccag gtgacgcggt atcgctcgag gagcagatcg accgatggcg gagctacctc
     8821 cgccaccggc aggcgatcca ttccgtcgac gtagcggagc tggaggacca cctgcgcgag
     8881 caggtagcgg tcctggtcga cgcggggctt gccaccgatg aagcgttcct ggtggcggtg
     8941 aagcgcatgg gcaagctcga cgacctctca cgggagttcg cgcgcgagca ctcggaccgc
     9001 ctctggaagc agctcgtcgt tgtcccctcc gatttcgggg
//
LOCUS       DATMVF010000124         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_159009, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000124 DATMVF010000000
VERSION     DATMVF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159009"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VN178_06140"
     CDS             <1..592
                     /locus_tag="VN178_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWS80587.1"
                     /translation="RSVSTIRREAGKMRGLIESLLTLTRGDEGPPMEVGRYDLGAVAK
                     EATETASNGKVSVEFVPTEHEIAATFDRGRVLQVASILIDNAVKYTPSGGSVAVRVEE
                     YDGGVALAVSDTGVGISEDQLPLVFERFYRADAARTEEGVGLGLSIARQIAEAHGGTV
                     AARSKPGVGSTFVLLLPRQKPDSSQGPPTKEAEDPR"
     gene            670..1362
                     /locus_tag="VN178_06145"
     CDS             670..1362
                     /locus_tag="VN178_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HWS80588.1"
                     /translation="MGIGTAINVLAVLAGGGIGTLAGARLPEGMRSTAMQAIGIVTLL
                     VGVSHFLEFDNVLVPLVSVILGLAVGELLDLDAQLRRLGDRLEERFSKGESPVSRAFV
                     TTSLLFCVGPLTVIGSLQDGLNGDYSLLALKSALDFIAALAFASVLGWGVLLSAGTVL
                     VVQGALTLGAAFLDAVVTGPMISSTTATGGILIFGLGLGLLDLKEIRVANMLPALLVA
                     PLLVAASPLWPL"
     gene            1454..1675
                     /locus_tag="VN178_06150"
     CDS             1454..1675
                     /locus_tag="VN178_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80589.1"
                     /translation="MEETDRHWRGSPLDVAGVGMDAYGIKEIVIEFDNGDEDIFTPKQ
                     REEFESYELHQMAVYLDSIGHSIRKGKKN"
     gene            1726..1911
                     /locus_tag="VN178_06155"
     CDS             1726..1911
                     /locus_tag="VN178_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80590.1"
                     /translation="MEQGVGVTRVVVRYSDGRVMNFVPDAGREAFSEDDMLELKKVLD
                     RISSTAEWAEINTRLGF"
     gene            complement(1991..2233)
                     /locus_tag="VN178_06160"
     CDS             complement(1991..2233)
                     /locus_tag="VN178_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80591.1"
                     /translation="MAQGVMRKIGELSAERERLLVREGTHHADSDDRERLQQIDHDLQ
                     VLWDLRRRELAGERVDLGEDFLDRYTVDPGRDAPGR"
     gene            2330..2737
                     /gene="mce"
                     /locus_tag="VN178_06165"
     CDS             2330..2737
                     /gene="mce"
                     /locus_tag="VN178_06165"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWS80592.1"
                     /translation="MLNRIYHLGYAVEDIEAASRFYEENFGAVPGEPEVVEEQGIVAT
                     TFQVGESSIELVQPTRPDSPVGKFLEKRGEGFHHVAFEVEDLSVTLAELKRNGVEVID
                     EEPRRGAGGTRMAFVHPRGAFGVLTELVELPHA"
BASE COUNT          493 a          852 c          917 g          497 t
ORIGIN      
        1 tcggagcgtc agcaccatcc gcagggaggc agggaagatg cgcggcctga tcgagtccct
       61 actaacgctt actcgcgggg atgagggccc tcccatggag gtagggcgtt acgacctcgg
      121 tgcggtcgct aaggaggcga ccgagaccgc atccaacggc aaggtgtccg tcgagtttgt
      181 ccccaccgaa cacgagatcg cggccacctt cgaccggggg cgcgtactgc aggtggcttc
      241 cattctcatc gacaacgcgg tcaagtacac gcccagcggc gggagcgtcg cggtcagggt
      301 ggaagagtac gacggcggcg tggcgctggc ggtctctgat acgggggtcg ggatctccga
      361 ggaccagttg cccctcgtct tcgagcgctt ctacagggcg gacgctgcgc ggaccgagga
      421 gggagtgggc ctcggactct ccatcgcccg ccagatcgcc gaggcccacg ggggcaccgt
      481 agcggccagg agcaagccgg gcgtgggctc cacgttcgtg ctcttgctcc cccgccagaa
      541 gccggattcg tcccaaggtc cccctacgaa agaggccgaa gaccccaggt aacgggccgc
      601 aggtacccgg cggccggtcc gacttactac ggatagggga gatccttttg tcatatactg
      661 cttgctcccg tgggaatcgg aaccgctatc aacgttctcg ccgtgctcgc cggcggtggt
      721 atcggcactc tcgcgggcgc caggctaccc gagggcatgc gctcgacggc gatgcaggct
      781 atcggtatcg taacgctgct cgtcggcgtc tcgcacttcc tggagttcga caatgtgctc
      841 gtgccgcttg tcagcgtgat cctgggtctc gcagtcgggg agctcctgga tctggacgcg
      901 cagctacggc ggttgggaga ccgtctggag gagagattct cgaagggcga gagtccggtc
      961 agccgggcat tcgtgacgac gagcctcctg ttctgcgtcg gcccgctgac ggtgatcggc
     1021 tcgctccagg acggcttgaa cggagactac agcctcctcg ctctcaagag cgccctcgac
     1081 ttcatcgcgg cgctggcctt cgcctccgtg ctggggtggg gcgttctgct ctcggccggg
     1141 acggtcctcg tggttcaggg cgcgctcacg ctcggggccg cctttctgga cgcggtcgta
     1201 accggaccca tgatctcgtc cacgaccgcg accggaggca tcctgatctt cggccttggc
     1261 ctgggtctgc tcgatctgaa agagatcagg gtggccaaca tgctccccgc tctcctcgtc
     1321 gcccccctgc tcgtcgcggc gagtcccttg tggccgttat gacttacgcg cgcgccgccg
     1381 atactctagt attcgtacgt gaaacggaag gcaaagaccg ggcggatctt cgcgcggtgg
     1441 agaggagcgg ctgatggaag agacggacag acactggagg ggaagtccgc tcgacgtggc
     1501 gggagtcggc atggacgcct acggcatcaa ggagatcgtc atcgagttcg acaacggcga
     1561 cgaggacatc ttcaccccga agcagcgcga ggagttcgag tcttacgagt tgcaccagat
     1621 ggcggtttac ctcgactcca taggccacag catccgcaaa ggcaagaaga actaaagggg
     1681 caagatttgc acggcataga gcgaaccagg aaggcaatcg ggccgatgga gcaaggcgtc
     1741 ggggtcacgc gtgtcgtcgt ccgttactcc gacggacgcg tgatgaactt tgtccccgac
     1801 gctggccgcg aggcgttctc cgaggacgac atgctggagc tgaagaaggt cttagaccgg
     1861 atctcctcga cggccgaatg ggccgagatc aacacgcgcc tgggattcta ggtaggtggg
     1921 cgggttcgac acccgccctg acacgcgaag cgcccctatg cctcgtcacg acggcgcctt
     1981 acgccccgcc ctacctgccg ggtgcgtcgc ggcccggatc gacggtgtaa cggtcgagga
     2041 agtcctcgcc taggtcgacc ctctcgcctg ccagctccct gcgtctcagg tcccatagga
     2101 cctgcaggtc gtgatcaatc tgttgcaggc gctcgcggtc atcggagtcc gcgtggtgcg
     2161 taccctccct taccaggagt cgctcgcgct ccgccgaaag ctctccaatc ttgcgcatca
     2221 caccctgtgc catcctcgcc tcctacccgt atgttcctcc ttgattctat cggatctgga
     2281 tagactacaa tcgttattcc tgtcggcgga gtgcgagcag gagagaagag tgctcaacag
     2341 gatctaccat ctcggttatg cggttgagga catcgaggcc gcctcgaggt tctacgagga
     2401 gaacttcggg gccgtgcccg gagaaccaga ggtcgtagag gagcagggga tagtcgccac
     2461 cacgttccag gtcggggaat cgagcatcga actcgtccag ccgacccgcc cggactcacc
     2521 tgtcgggaag ttcctggaga agcgcggcga gggcttccac cacgtcgctt tcgaggtcga
     2581 ggatctcagc gtgactctgg ctgagctcaa gcgtaatggc gtcgaggtca tagacgagga
     2641 gccgaggagg ggggcagggg ggaccagaat ggccttcgtc caccccaggg gtgccttcgg
     2701 ggtgctcacc gagctagtag aattgccaca cgcctgagac gagcggggag gacgagccg
//
LOCUS       DATMVF010000125        20700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_565291, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000125 DATMVF010000000
VERSION     DATMVF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 20700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20700
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565291"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VN178_06170"
     CDS             <1..563
                     /locus_tag="VN178_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWS80593.1"
                     /translation="FERLFGERYSILDFEEAVMVAVDSSEPDLDDGRVGREHYEFIYE
                     TFAMAEDKLKIFVIHHHLIPIPGTGRERNIIYDAGDVLELLADTEVDLVLSGHKHVPY
                     SWKLENMFIVNAGTASTTRLRGNTRPCYNVIEIEDGRVRVFRKYPFKDREMIVDFDSA
                     THQYLHFEEGQADGRQEDLRKRRTLS"
     gene            570..1886
                     /locus_tag="VN178_06175"
     CDS             570..1886
                     /locus_tag="VN178_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HWS80594.1"
                     /translation="MRALFLIDGEHYPPVVLDAMQSVEQSLHAVGVAAAFLGGTEKIR
                     EGTDYGVPLVEGKDPVSAVQKALSEYEVDIVVDLSDEPVIGYRERMKIASLALHAGAR
                     YLGSDFELKPPDFRPVSTKPSLAVIGTGKRVGKTAISGYLARLLAREGFDPGVVSMGR
                     GGPRHPEVIEGHEMEVGSEYLLEALGRGAHAASDYYETAALSRVTTVGCRRCGGGLAG
                     EPFISNVLEGAGIANGLETSVTLFDGSGAAMPPVEVERRVLVAGANQDPEYIVGFLGT
                     YRLLLSDLVLLTMSEEPMADGEKVRGLIAAIGKLRPDLVVIPTVFRPRPVGEVEGLRV
                     GYVSTAPPAVLDTLSRHLEEYYGCEVVAASGNLSDRKRLGADLEEMSGVEAYLTEIKA
                     AAVDVVTRRGTAEGKPVFYCDNDPVGEGLDRELLRLAREAVANRVP"
     gene            2090..3073
                     /locus_tag="VN178_06180"
     CDS             2090..3073
                     /locus_tag="VN178_06180"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HWS80595.1"
                     /translation="MEYAAVTEQVALKVARLEGSGDSERAYTLATEAFREELERVPVS
                     GRVAVLREQRLDEMGRLRAPKSHPGIVGVDDSLGNRGPEADLAVEPIEGLQLLVKGQE
                     GSISAVAAAPRGSMLRTPDMYMSKLIVGPGAKGNIDIDAPVSENIEAIAKALGRRPQE
                     INVAVLDRDRHETLIEEIRTTGARIQIRPEGDLTPAIAVGMRGADLHAIIGIGGAPEG
                     ILAAAVMKCLGGEMQARMWPTQRSQVEKLEEYGFADPERKLTLDDLIRGTDVLFVATG
                     ITPGETLNGVRYFRGGARTHTVVMSTEPRVVRFMDTIHALEPEAGRRGFQL"
     gene            3140..4660
                     /gene="rho"
                     /locus_tag="VN178_06185"
     CDS             3140..4660
                     /gene="rho"
                     /locus_tag="VN178_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWS80596.1"
                     /translation="MTERATLERKRLSDLHQIASQLGIKQYRKYRKPELAEMIYQVAT
                     EQAASQQNETATNGGSSATATQEAPVTTDAPNGAPAETPVAPVDGFGEADTNLATTEQ
                     VERQRQERGRRDRKNGRQDRRQEQRGDRDGKGGEENVQSGILDVLPDGFGFLRTGGYS
                     QSKGDVYVSTSQIKRFNLRRGDYIVGQIRPAREGEKYPAMVRIETVNGQEPQKGRWRP
                     NFDDLTPLYPMERLRLEWKPNDIAPRVIDLVAPIGKGQRGMVVSPPKAGKTTILKQIA
                     QSIAANYPETKLFVVLADERPEEVTDWQRSVEEAEVVASTFDQPADNHIAVAELVLER
                     VKRLVEEGEDVVVLLDGITRLSRAYNLAAPASGRILSGGVDSAALYPPKKFFGAARNI
                     ENGGSLTILATALVETGSRMDEVIFEEFKGTGNMELRLDRKLANRRIYPAINIEDSST
                     RKEELLMEPAEAQRVWQVRSILNALDTSQKIELLIQKLKETRTNPEFLRELQRVRG"
     gene            4766..4981
                     /gene="rpmE"
                     /locus_tag="VN178_06190"
     CDS             4766..4981
                     /gene="rpmE"
                     /locus_tag="VN178_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWS80597.1"
                     /translation="MKTGIHPEYTKTLVRCMCGNEFETRSTAGDTITVDLCSECHPFY
                     TGKQRIVDTGGRVQRFQNRRQARQNRQ"
     gene            4993..5907
                     /locus_tag="VN178_06195"
     CDS             4993..5907
                     /locus_tag="VN178_06195"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWS80598.1"
                     /translation="MRVGGQAIMEGVLMRSPNFWGMAVRTPSGDMDLRAERFRSVTRR
                     SKLFRLPIIRGALSLGETLWLGMKALTISTNIALGEEQELSKKEIAGTLIFALVLGFG
                     LFLVAPVLGTKGLGSFLGNSIENPVIFNLVEGFIRIAIFIAYLLAITLLSKDIKRFFA
                     YHGAEHKAIKVYERDEELVPANARKLDTSHVRCGTSFVLYVLVLSILVFSLLGVEGWL
                     YMLASRVIVIPLVAGIAFEFIMWSARHQDSALVQALVWPGLQMQKLTTREPSDDMVEI
                     AMASLKKVLSMEKEAEIEPNEATNKLVI"
     gene            5924..7003
                     /gene="prfA"
                     /locus_tag="VN178_06200"
     CDS             5924..7003
                     /gene="prfA"
                     /locus_tag="VN178_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWS80599.1"
                     /translation="MKLAEETLGRYDALTQELSDPNIFDDQRRYAAVAREHARMRRGA
                     ELSRELLQTIEAGRDARELIPQAENAEEREFFAAEARDAEERIEDLSERIRTELIDRD
                     PNDDKDVIIEIRAGAGGDEAALFAGELHDMYTRYADRLGYKHRTLSSSPAEVGGYREV
                     AVEISGDRAYSVFKHEGGTHRVQRVPKTESQGRIHTSTATVAVLPEAEDVEVQISAGD
                     LEVDVYRSSGPGGQSVNTTDSAVRITHKPTGLVVTCQNEKSQLQNKEQAMRILKSRLL
                     EREMRERAEQEGALRQAQVGTGDRSAKVRTYNFPQGRITDHRVGVTSHNLEATLGGDL
                     DEFTQALAAKERADKLAAETAGTPG"
     gene            7008..7877
                     /gene="prmC"
                     /locus_tag="VN178_06205"
     CDS             7008..7877
                     /gene="prmC"
                     /locus_tag="VN178_06205"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWS80600.1"
                     /translation="MRAGTGRASARDAARSAAARLERAGVPEPVASAEVLLSELLGVG
                     RAELAASAVQLTAEQASLYEAWISRRMTREPVQRILGYAYFRNLVLDLNEDTLIPRPD
                     TESVVDAALEFIDSRGYPCVVLDVGTGSGAIAISIAQERPNCEVHASDISETALRIAR
                     QNAVRNNVAVRFHEADVASGLTFLNGSIDLLVSNPPYVDEISAPRRLAPEVREWDPPV
                     ALYSGTDELGFFRRIFSETPPLLKRGADVVLEIGDGQAEKVQELGSRSGFVPLGVRND
                     LAGTPRAVLLKWG"
     gene            7874..8440
                     /locus_tag="VN178_06210"
     CDS             7874..8440
                     /locus_tag="VN178_06210"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWS80601.1"
                     /translation="MRTVSPQEAVAALRAGNVALVPTETVVGLVAAESGLSRIHEIKG
                     RDANKPIALLCVSAEAAFGLAPNVPPIARELAELYWPGPLTLVLDLPSGGTIGVRVPA
                     GEAVRDLLEAYGAPLFATSANLSGEPATAATDDVDPRVLEAVDIIVRGERGTGEASAV
                     VDLSGGNVRLVRAASELSAQRLAQLAHR"
     gene            8457..8672
                     /locus_tag="VN178_06215"
     CDS             8457..8672
                     /locus_tag="VN178_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80602.1"
                     /translation="MGEEQFSYRITKNGKLIVHWHGKQGKREIILKGARAENLIRNLP
                     AMTPDQQQLALARATGNFKRGNERQGR"
     gene            complement(8864..9079)
                     /locus_tag="VN178_06220"
     CDS             complement(8864..9079)
                     /locus_tag="VN178_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80603.1"
                     /translation="MLTKFHDVLAGLELVRQDYLATLSELRANGSLTELDRNLLRREL
                     AVEHRVRCRQEWKAIIIQLKADQDVTT"
     gene            9316..9843
                     /locus_tag="VN178_06225"
     CDS             9316..9843
                     /locus_tag="VN178_06225"
                     /inference="COORDINATES: protein motif:HMM:NF037679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS80604.1"
                     /translation="MEAAERVVARDGVARLTLDAVAGKADVSKGGLLYHFPNKDALIS
                     AMVLRWVDGFERDIGQRLEVEEAGAAGSWARAYAGATFEPGETQETQPRELVALLAAM
                     ATDPALLEPLRRRFEAWQARAEDDGLDLALATLLRLAADGLWFADLFGLAPPAGELRG
                     RVLREINGLARGEEG"
     gene            9843..10166
                     /locus_tag="VN178_06230"
     CDS             9843..10166
                     /locus_tag="VN178_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HWS80605.1"
                     /translation="MSWLLLVVSIVLEVLGTVSMKLSEGFSRPLYSALVALFYALSIA
                     GLALVIKHIDVSVAYAVWSGLGTAFIAMAGVSVFGEAFTVVKVVALVLIVVGVVMLNM
                     VSPMH"
     gene            10477..11700
                     /gene="glyA"
                     /locus_tag="VN178_06235"
     CDS             10477..11700
                     /gene="glyA"
                     /locus_tag="VN178_06235"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWS80606.1"
                     /translation="MIGPYTKAVDSEVQEALEGELDRQRTTIELIASENFTSPAVLAA
                     QGSVLTNKYAEGYPGKRYYGGCEQVDKVENLAIERAKKLFGAEHVNVQPHSGSQANEA
                     AYAALVDPGDKVLSMDLAHGGHLTHGMKINFSGRTYDFVHYGVGEDGYIDYDQVRDIA
                     LSERPKMILAGASAYPRIFDFEKFREISDEVGAFFLTDMAHIAGLIAGGVHPSPIPYS
                     EIVTTTTHKTLRGARGGMILCREEFAKKVNSRVFPGMQGGPLMHAIAGKAVALGEALQ
                     PEFEDYARQIVKNCRALAEGLMEGGLDLVSGGTDNHLILVDLKDTGVTGKELEDSLEA
                     VGVTCNKNMVPNDPEPPTVTSGIRLGTAAMTTRGMGEDEMQEIADIIVATARGETDGL
                     RDRAKALTEAYPLYE"
     gene            11693..12340
                     /gene="upp"
                     /locus_tag="VN178_06240"
     CDS             11693..12340
                     /gene="upp"
                     /locus_tag="VN178_06240"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HWS80607.1"
                     /translation="MSETRPDVKNLHVVDGPLSRHKLAVLRDERTSTADFRQAMKELA
                     LIMVAEATRYMPTRRVRIRTPLTETEVEEISGPVCLVPVLRAGLGMLDGALDLLPTAT
                     VGFMGLQRDEETARPIEYYVNLPRNLDEYLVLVLDPMLATGGSLSATIGKLKEEGATW
                     ISCLHAVAAVPGVERVSNEHPDVTFYAAAVDPELDDCSFIVPGLGDAGDRLYGTL"
     gene            12327..12815
                     /locus_tag="VN178_06245"
     CDS             12327..12815
                     /locus_tag="VN178_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HWS80608.1"
                     /translation="MEPFEDPAADTSNSHEALKRVRPSWDEYFMRIAHEVATRSTCPR
                     LAVGAVIVRDKRILTTGYNGSPSGMPHCDEVGCLIRIVDGRESCQRTLHAEQNAIIQA
                     AYHGVSVTGASIYCTHQPCLMCVKMTMNAGIEEVHYAGGYPDPLAMELAAEGGLAMVR
                     FA"
     gene            complement(12973..13602)
                     /locus_tag="VN178_06250"
     CDS             complement(12973..13602)
                     /locus_tag="VN178_06250"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS80609.1"
                     /translation="MSRDRVPRRRRKPEEAERAILAAARAFLEEHPFREMTIEGVMIR
                     TGLSRPAFYAYFRDRYEVVTRLLEGIGGLLFALDWRWLSGGEVGEEAKEVLVEALRAG
                     SQTFVEYGPVLRAIADAAGYDERVEQVYRYGLIERLVGAVAVRISRDVEAGLSSADLD
                     PEETARALALMTERYLLDAFGHPERRPSGEESVLVFRTLEAIWVRTLYG"
     gene            13876..14100
                     /locus_tag="VN178_06255"
     CDS             13876..14100
                     /locus_tag="VN178_06255"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWS80610.1"
                     /translation="MPDTRSQRNQASGEYARFVGLGFTFMVIIAVSTAGGYLLDWLFG
                     TLPLLLLVGLLVGFGAALTYLFVTLSRVGR"
     gene            14100..14516
                     /locus_tag="VN178_06260"
     CDS             14100..14516
                     /locus_tag="VN178_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80611.1"
                     /translation="MSAHWRLALGGAAATAVMSVGAAAIVGYYFGAVYAGAFLYGAGV
                     GLVSFTSIAITAWLLGGKMSGERVLLGVAVYFGRLVFAVVAVGAPIFLASWPALALIG
                     GFAGVYVVENVLLLIGAVKVRGAYGRGAGVGDRNGG"
     gene            14525..15313
                     /gene="atpB"
                     /locus_tag="VN178_06265"
     CDS             14525..15313
                     /gene="atpB"
                     /locus_tag="VN178_06265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWS80612.1"
                     /translation="MTLALLQVQGAELRKEIIHAWESGQHAIVHLSLFGIDISITKAV
                     VFLWIGAALTFLIMFIGARTLKERPGAYQVIVEEIYAFGRNSMGGQLGEEGRKWFPYT
                     LSLFIFLLVLNLLGLVPNSFPVTSSISFTLVLALLTFIITQVVGIRRNGFGGYVKAFV
                     PPGMPAKPIMVPFMFFIEWVSELSKPLTLAMRLYANILAGHLLIFIFLGFILYTGTPL
                     MAAVSVPVAVVLFGFEIFVAVLQAYIFAILTQVYIEIALYRQEH"
     gene            15351..15626
                     /gene="atpE"
                     /locus_tag="VN178_06270"
     CDS             15351..15626
                     /gene="atpE"
                     /locus_tag="VN178_06270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HWS80613.1"
                     /translation="MEILAFIPPLLALLQGSSQLYLVGFGFAAGTAVIGSGIGMGYLF
                     GQTIASIHRQPEAFELSRTWLFVGIGLVEAQALFGLAFALLIGLQIF"
     gene            15662..16186
                     /gene="atpF"
                     /locus_tag="VN178_06275"
     CDS             15662..16186
                     /gene="atpF"
                     /locus_tag="VN178_06275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HWS80614.1"
                     /translation="MDETGIFDILNTSLIFWEVIAFFILLFLLVRYVYPPIREQIQER
                     QSEIERAIDEAEKTRAEARELLAEYRRQIEEARGEGRQILEEARKQAEAQRERTKREA
                     REEGDRIIQRAREEIDRERESALREVRREVADMVVQASEQVIGRSIDRDEHERLISQA
                     LDDLEAEMAESGTA"
     gene            16183..16722
                     /gene="atpH"
                     /locus_tag="VN178_06280"
     CDS             16183..16722
                     /gene="atpH"
                     /locus_tag="VN178_06280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HWS80615.1"
                     /translation="MSAVSTYAGALFEAARERSELEEVLSDLGEFVSALHESEELRLF
                     FYGGQVPERQKRRAIDGLTEGMKTSSTNFLKVLVDNGREEILEEVLGRYEQLVKEHLG
                     RIEVGVTTAVELSEEQAGRLKERLGRVLEGREVILETNVDPELIGGAVFRFGGRMMDG
                     SIRGRLESLREGMLERSVV"
     gene            16722..18326
                     /gene="atpA"
                     /locus_tag="VN178_06285"
     CDS             16722..18326
                     /gene="atpA"
                     /locus_tag="VN178_06285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWS80616.1"
                     /translation="MGRLRPEEISSILKGAITDYENRVRTEEVGQVLEVGDGIARVHG
                     LTNVMYQEMLEFEDTRGEKITGLALNLEEDNVGVIVAGDDTHIQEGNTVRRTERLASV
                     PVGEGVLGRVIDALGRPLDGKGEIEAEETLPVEIVAPGIIRRQPVKQPLQTGIKAIDS
                     MIPIGRGQRELVIGDRKTGKTSILLDTIINQADQDVKCVYVAIGQKDSTVAGVVAALE
                     ESGALEYTTVVNASAAQSATLQYLAPYAGCAIGEFFMNRGEDALLIYDDLSKHAVAYR
                     QMMLLLRRPPGREAYPGDVFYLHSRLLERAAKLSEDSGGGSLTALPVIETKAGDISAY
                     IPTNVISITDGQIFLDSDLFNSGQRPAIDTGNSVSRVGSSAQIKAMKSVAGTLRLDLN
                     QYRELASFAQFGSDLDRATQQTLNRGERLTAILRQEELDPMPVEEQVVVLFAATNGYL
                     DEVDADDVMDWERQFRDFMRDAHGDLLDSIRTEQELSDENEEKLREAVEHFDSNFEPE
                     QTSLVDISVEDENGEEGDQTREEEGS"
     gene            18330..19190
                     /gene="atpG"
                     /locus_tag="VN178_06290"
     CDS             18330..19190
                     /gene="atpG"
                     /locus_tag="VN178_06290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HWS80617.1"
                     /translation="MPSARDLQRQINTVKNQARVFSALQTVSSVKFRKAEARVKRARP
                     YAENVEQMMRDVAANATGDLPLLTGREVNRVAVCTLTADRGLAGGFNAQVLRRTVRLR
                     EEKDSDALQVASGRKAVAFFRFRRVELAEVFTGFTDNPTYENAREIGRTLTRIFDDED
                     ADEVYLVYNRFESALNQRPVVNRLLPVAREEDEEEDSNNSYMDYVPDAETVLKRLVPR
                     YVETMVWQALLEGAAGEHGARMTAMKNATDNANDLVRDLTLQMNKARQAQITQEILEI
                     VGGAEALSAG"
     gene            19206..>20700
                     /gene="atpD"
                     /locus_tag="VN178_06295"
     CDS             19206..>20700
                     /gene="atpD"
                     /locus_tag="VN178_06295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWS80618.1"
                     /translation="MSESESGQTFNRTTFGGERGEVDNGHAATEQESGRDGHEERGEG
                     VGTVVEVKGPVVDVRFPPEDIPEIFNAVTVPIEKDGETIQLTLEVQQLLGDDMVRTVA
                     MASTDGLRRGVDARDTGRPIAVPVGKNVLGRVLDVLGQPVDEQGPVEDPDDYWPIHRP
                     SPKFEDLTTQAEQFVTGIKVIDLLCPVRRGGKVGLFGGAGVGKTVVIQELINNIALQY
                     EGTSVFAGVGERTREGNDLYGEFEEAGILSNVGLVFGQMNEPPGARFRVGLTGVTLAE
                     YFREELGQDVLFFVDNIFRFVQSGNEVSALMGRMPSEVGYQPTLGTEMGALQERITST
                     KKGSITSFQAVYVPADDFTDPAPFTVFAHLDSTVELSRSLSAQGIYPAVDPLTSSSTI
                     LDPSVVGDEHYQVAQEVKNILQRYKELQDIIAILGIDELSEEDKVIVGRARRLQRFLS
                     QPFFVAEQFTGLEGKFVELEDTIRSFKEVVEGNHDELPEQAFYLVGNI"
BASE COUNT         4105 a         6463 c         6643 g         3489 t
ORIGIN      
        1 acttcgagcg gctcttcggg gagcgctact cgatactgga cttcgaagag gccgtcatgg
       61 tcgccgtgga ctcctccgag cccgacctcg acgacgggcg tgtcggccgc gagcactacg
      121 agttcatcta cgagaccttc gccatggccg aagacaagct caagatcttc gtcatccacc
      181 accacctgat ccccatcccc ggaacgggga gggagcgcaa catcatctac gatgctgggg
      241 atgtgctcga gctactcgcc gacacggagg tagacctcgt cctctcgggg cacaagcacg
      301 tgccttactc ctggaagctc gagaacatgt tcatcgtcaa cgcggggacg gcctcgacca
      361 cccgcctcag gggcaacact cgtccctgct acaacgtaat agagatcgag gacgggcggg
      421 tgcgggtctt ccgcaagtac cctttcaagg accgcgagat gatagtggac ttcgactccg
      481 caacccacca gtacctgcat ttcgaagagg gccaggccga tggcagacaa gaggaccttc
      541 gcaagcggcg aacgctgagc tagaacttcg tgcgggcgct gttccttatc gacggcgagc
      601 actatcctcc agtcgtcctc gacgcgatgc agagcgtaga gcagtctttg cacgccgtgg
      661 gagtcgccgc agcctttttg ggcggcaccg agaagatcag ggaaggcacc gattacggcg
      721 tcccgctggt cgagggcaaa gaccccgtct ccgcagtaca gaaggcgctc tccgaatacg
      781 aggtcgacat cgtcgtcgac ctctccgacg aacccgtgat cggctacaga gaacggatga
      841 agatagcctc tctcgccctc cacgccgggg cccgctacct gggaagcgac ttcgagctga
      901 aaccgccgga ctttcgcccc gtctcgacca agccttctct ggcagtaatc gggacgggca
      961 agagggtggg gaagacggcg atctcgggct acctggcgcg tctcttggcc cgtgagggat
     1021 tcgaccccgg cgtagtctcg atgggccgcg gtgggccgcg gcaccccgag gtcatcgagg
     1081 gccacgagat ggaggttggc agcgagtacc tgctcgaggc gctcggacgt ggggcgcacg
     1141 ctgcgagcga ctactacgag acggcggccc tcagcagggt caccaccgtc ggatgccgca
     1201 ggtgcggcgg cggtctggca ggcgaaccct tcatctcaaa cgttctggaa ggggccggga
     1261 tcgcgaacgg cctcgagacc agcgttaccc tcttcgacgg ctcgggggcg gccatgcccc
     1321 cggtcgaggt ggagcggcgc gtgctcgtcg caggggccaa ccaggacccc gaatacatag
     1381 taggctttct cggcacctac cgcctgttac tctcagacct ggtcctcctc accatgagcg
     1441 aggagccgat ggcagacggc gagaaggtcc gcggtctcat tgctgcgata ggcaaactgc
     1501 gtcctgatct ggtcgtgatc cccacggtct tcaggccccg tccggtcgga gaggtcgagg
     1561 ggttgcgggt cggatacgtc tcgacggcgc cgccagccgt gctcgacacc ctctccaggc
     1621 acctcgagga atactacggt tgcgaggtgg tcgccgcctc ggggaacctc tccgacagaa
     1681 agcgtctcgg cgccgacctt gaagagatgt cgggagtaga ggcgtatctt accgagataa
     1741 aggcggcggc ggtggacgtc gttacccgcc ggggtactgc ggaggggaag cccgtcttct
     1801 actgcgataa cgaccccgtg ggcgagggcc tggaccggga attgctcagg ctcgctcggg
     1861 aggcggtggc aaacagggtg ccttgagccg gtggtcgact taccggcgct ggtgtataat
     1921 ctcggtgtca ggtacgctgg ttcttcggga ccactccgca ccgattcgac gcaggcccag
     1981 ccacgagagc gttccgtggc gtgcggtcta tcttgatggg tcatgggagg ttcggtgaga
     2041 gcccattaac gttactgatt ccccttcggg aaccatcgcc tcggctgccg tggaatacgc
     2101 tgccgtaacc gagcaggtcg cgctcaaggt ggcgcgcctc gaaggttcgg gagattcgga
     2161 gcgggcctac acgcttgcta cggaggcttt ccgcgaggag ctggagcgcg tgccggtctc
     2221 cggaagagta gcggtgctgc gcgagcagcg tctcgacgag atggggcgcc tcagggcgcc
     2281 caagagccat cccgggatag tcggtgtgga cgactcgctc ggaaacagag ggcccgaagc
     2341 cgacctggcg gtcgagccca tcgagggttt gcagttgctc gtaaaggggc aggaaggttc
     2401 gatctcggca gtcgctgccg ccccaagggg gagcatgctc aggacccctg acatgtacat
     2461 gagcaagctg atcgtcggcc ccggtgcgaa gggcaacatc gacatagacg cccccgtctc
     2521 ggaaaacata gaggctatag cgaaggccct tggtcgaagg ccccaagaga tcaacgtcgc
     2581 cgtgctcgac cgcgacaggc acgagaccct gatagaggag atccgaacga cgggagcccg
     2641 catccagata aggccggagg gcgaccttac tcctgccata gccgtgggta tgcgtggggc
     2701 cgacctgcac gccatcatcg gtattggagg cgcccccgag ggtatcctcg cagccgccgt
     2761 catgaagtgc ctcggcgggg agatgcaggc caggatgtgg ccgacgcaac gctcccaggt
     2821 cgagaagctc gaggagtacg gctttgcaga ccccgagcgt aagcttacgc tggacgacct
     2881 gatccggggc accgacgtgc tattcgtagc gacagggatc acccccggcg agaccctcaa
     2941 cggggtaaga tacttccggg gtggtgccag gacgcatacg gtcgtcatgt cgaccgagcc
     3001 tcgtgtggta agattcatgg acaccatcca tgccctagag cccgaagcag ggcggcgggg
     3061 tttccagctt tgacaaaccc gtgaagaagt tcgtcgaaag acattctcgc atcgtaaaca
     3121 ggagaggtgg agtacaaaca tgaccgaaag agctaccctg gagcgcaagc gcctgagtga
     3181 tttgcaccag atcgcctcgc agctagggat aaagcagtac cgcaaatacc gcaagccgga
     3241 gctggcagag atgatctacc aggtcgcgac cgagcaggcg gcgagccagc aaaacgaaac
     3301 cgcgaccaac ggaggctcct cagcaaccgc aacgcaggaa gcccccgtaa cgacagacgc
     3361 ccccaacggc gcaccggcgg agacgcctgt ggcgcccgta gacggcttcg gcgaggccga
     3421 cacgaacctc gctaccaccg aacaggtcga gcgccagcgc caggaacgcg gccgccgtga
     3481 ccgtaagaac ggccggcagg accgccgtca ggagcagcgg ggcgataggg acggcaaggg
     3541 cggcgaggag aacgtgcaga gcggtatcct cgatgtgctg cccgacggct tcggcttcct
     3601 aaggacgggg ggctacagcc agagcaaggg cgacgtctac gtctcgacca gccagatcaa
     3661 gcgcttcaac ctgaggcgtg gcgactacat agtcggccag atccgtcccg cccgcgaagg
     3721 cgagaagtac cccgcaatgg tacgcataga gaccgttaac ggccaggagc cccagaaggg
     3781 ccgttggcgc ccgaacttcg acgacctgac gccgctgtat ccgatggagc ggctgcgcct
     3841 ggagtggaag ccgaacgaca tcgccccccg cgtcatagac ctggtcgccc ccataggcaa
     3901 gggccagcgt ggcatggtcg tctcgccccc caaggccggc aagaccacga tcctcaagca
     3961 gatagcccag tccatcgcgg ccaactaccc ggagacgaaa ctcttcgtcg tgctcgccga
     4021 cgagcgcccc gaggaggtca ccgactggca acgcagcgta gaggaggccg aggtcgtcgc
     4081 ctcgaccttc gaccagcccg ccgacaacca catcgccgtc gcagaactcg tcctggagcg
     4141 cgtcaagcgc ctggtcgagg agggagagga cgtcgtcgtc ctgctcgacg gcattaccag
     4201 gctctctcgt gcatacaacc tcgccgctcc cgcttccgga aggatcctct ccggcggcgt
     4261 cgattcggcg gcgctctacc cgcccaagaa gttcttcggg gccgcgcgca acatcgagaa
     4321 cggcggatcc ctgaccatcc tggcgaccgc gctcgtggag acgggaagca ggatggacga
     4381 ggtcatattc gaggagttca agggcacggg caacatggag ctcaggctcg acaggaagct
     4441 cgcgaaccgc aggatctacc ctgccatcaa tatcgaggac tcctcgacgc gcaaggagga
     4501 actgctcatg gagccggccg aggcccagag ggtctggcag gtccgcagca tcctcaacgc
     4561 cctcgacacc agccagaaga tagagctcct gatccagaag ctcaaagaaa cccgcacaaa
     4621 ccccgagttc ctgcgtgaac tccagcgcgt ccgcgggtag gaccgcttcg cagtccgggg
     4681 agtagggttt gcttcctttc ctactccctg ttccctagag ttgctgcggt aaaatgagtg
     4741 ggtatcgtcg agaatggagg ttcgaatgaa gactggtata catccggagt atacgaagac
     4801 gctggtgcgt tgcatgtgcg gcaatgagtt cgagactcga tccacggcgg gggacacgat
     4861 cacggtggac ctctgctcgg agtgccatcc gttctacacc ggcaagcagc gcatagtaga
     4921 caccggcggt agggtgcagc gcttccagaa tcgccgccag gctcgccaga acaggcagta
     4981 aggccgaacg cggtgagggt cggcggacag gccatcatgg agggcgtcct gatgcgctcc
     5041 cccaacttct gggggatggc cgtccgcacc ccttcggggg acatggacct gcgggccgag
     5101 cggttccgtt ccgttacgcg gcgtagcaag ctgttccgtc ttcccattat caggggcgcc
     5161 ctctccctcg gggagaccct gtggttgggg atgaaggctc tcaccatatc cacgaacatc
     5221 gcgctcggcg aggaacagga gctctccaag aaagagatag cgggtacgct catcttcgcg
     5281 ttggtgctcg gtttcgggct cttcctcgtc gcccccgtgc tggggaccaa ggggctcggc
     5341 tctttccttg gtaacagcat cgagaacccg gtcatcttca acctcgtcga gggattcata
     5401 aggatcgcga tcttcatcgc ctacctgctg gcgatcacgc tcctcagcaa ggacatcaaa
     5461 cgcttcttcg cctaccacgg cgcggagcac aaggcgataa aggtctacga gcgggacgag
     5521 gaactcgtcc ccgcaaacgc gcgcaagctc gacaccagcc acgtccgctg cgggacgagc
     5581 ttcgtgctct acgtgctcgt gctctccatc ctggtcttct ccttgttggg ggtcgaggga
     5641 tggttgtaca tgctggccag tcgggtgatc gtcatcccgc tggtggcggg catagcgttc
     5701 gagttcatca tgtggagcgc gcgccaccag gacagcgcgc tggtgcaggc gctcgtctgg
     5761 cccggcctgc agatgcagaa gttgacaacc cgggagccat cggacgacat ggtcgagatc
     5821 gcgatggcct ccctgaagaa ggtcctctcc atggagaaag aagccgagat cgagccgaac
     5881 gaggctacga acaaactggt gatttgaatt ggatagtcag gtagtgaagc tggccgagga
     5941 gacgctcggc cgctacgacg cgttaacaca agaactctcg gacccgaaca tcttcgacga
     6001 ccagcggcgc tacgccgctg tcgccagaga gcacgcgcgc atgcggcgcg gggccgagct
     6061 ctccagggag ctgcttcaga cgatagaggc cgggcgcgac gcacgggagc tcatccccca
     6121 ggccgagaac gcggaggagc gggagttctt cgcggccgaa gcgagggatg ccgaggagcg
     6181 gatcgaagac ctctccgagc gtatcaggac cgagctcatc gaccgcgacc ccaacgacga
     6241 caaagacgtc atcatcgaga tccgggccgg cgccggtggc gacgaggcgg ccctcttcgc
     6301 cggcgagctc cacgatatgt acacccgcta cgccgaccgc ctcggctaca agcaccgtac
     6361 cctgtcgtcg agcccggccg aagtcggcgg ctacagggag gtcgccgttg agattagcgg
     6421 cgaccgcgcc tactcggtct tcaagcacga aggcgggacg cacagggtcc agcgggtgcc
     6481 caagaccgag agccagggcc gcatacacac ctcgacggct accgtggccg tcttgccaga
     6541 ggccgaggac gtggaggtcc agatcagcgc gggagacctg gaggtcgacg tgtaccgttc
     6601 cagcggtccc ggcggccaga gcgtgaacac cacggactcc gcggtcagaa tcacgcacaa
     6661 gccgacgggc ctggtcgtca cctgccagaa cgaaaagagc cagctccaga acaaggagca
     6721 ggccatgcgg atcctgaagt ccaggctctt agaacgcgag atgcgcgaga gggccgagca
     6781 ggaaggcgcc ctccggcagg cgcaggtcgg taccggcgac cgctcggcga aggtccgtac
     6841 gtacaacttc ccgcagggac gcatcaccga ccaccgggtc ggcgtcacct cccacaacct
     6901 cgaagctacc ctcggcggcg acctcgatga gttcacgcag gccctcgccg ccaaggagcg
     6961 ggcggacaaa ctcgccgccg aaaccgcagg aaccccgggc tagaacggtg cgtgccggca
     7021 caggccgagc cagcgcccga gacgctgccc gtagcgccgc cgccagactc gagcgggcag
     7081 gagtccccga gcccgtcgca tccgccgaag tcctgctctc cgagctgctc ggcgtagggc
     7141 gcgcggagct cgccgcctcg gcggtgcagt tgacggccga gcaggcgtcg ctttacgagg
     7201 cctggatctc ccgccgcatg acgcgtgaac ccgtgcagcg tatcctgggc tacgcatatt
     7261 tcaggaacct ggtcctcgac ttgaacgaag acaccctcat cccgcgcccc gacaccgaga
     7321 gcgtcgtcga cgcggcactg gaattcattg actcccgagg ctatccgtgc gtcgtactcg
     7381 acgtcggaac cggctcggga gccatcgcca tctccatcgc ccaggaacgc cccaactgcg
     7441 aggtacacgc ctcggacatc tctgagaccg cgcttcgcat cgcacgccag aacgccgtca
     7501 ggaacaacgt ggccgtcagg tttcacgagg ccgacgtggc ctccggcttg accttcttga
     7561 acggttcgat cgacctcctc gtctccaatc cgccctacgt ggacgagatc agtgcccccc
     7621 gccgtctcgc tccggaagtc agagaatggg acccacccgt ggccctttac agcggtaccg
     7681 acgagctagg atttttccgc cgcatcttct cggagacgcc cccgctgctg aaacgtggcg
     7741 ctgacgtggt tctggaaatc ggcgacggac aggccgagaa ggtgcaagaa ttgggctcta
     7801 gatccggctt cgtaccgctc ggcgtacgca acgatctggc aggcacaccg cgggccgtgc
     7861 tgctgaagtg gggatgagaa ccgtctcgcc ccaagaggcc gtcgccgccc tgagagcggg
     7921 caacgtcgcc ctcgttccca cggagacagt agtcggcctc gtcgccgccg agtcgggcct
     7981 atcgaggatt catgagatca agggcaggga cgcgaacaaa cccatagccc tcctctgcgt
     8041 ctcggcggaa gcagcctttg ggttggcgcc gaacgtgcct ccgatagcta gagaactcgc
     8101 ggaactctac tggcctggcc ccctaacgct agtcctggat ctcccctccg gaggcaccat
     8161 cggcgtaagg gtgccagccg gcgaggccgt gcgggacctg ctcgaggcct acggcgcccc
     8221 actcttcgcc accagcgcca acctctccgg ggaaccggcc acggcggcga ccgacgatgt
     8281 ggacccgcgc gtgctcgagg ccgtcgacat catcgtcagg ggggaacgag gtaccggtga
     8341 agcctcggcg gtcgtcgacc tttcgggagg caacgtaagg cttgtgcgtg ctgccagcga
     8401 gctgtccgca cagaggttgg cccagctcgc acaccggtga gggtggctgg agaatcatgg
     8461 gcgaggaaca gttttcgtac cggatcacca agaacggcaa gctaatcgtc cactggcatg
     8521 gcaagcaagg caaacgcgag atcatactca agggagcacg ggctgagaac ctcatcagaa
     8581 acctgccggc aatgacccct gaccaacagc agctcgcgct cgcccgcgcc acgggcaact
     8641 tcaagcgcgg caacgagcgt cagggtcgct agcgctccgg gcatcgaggt acacgtatca
     8701 ggattcgcgc tggacagtga agatctcgcc ctggacccgc ttgggtagag ataacgagtc
     8761 tgccagaaac aaacaccccc gttcgcgccc catgggggcg ggcgctgttc gcttaccagg
     8821 agacccggct gcagttctcg gtctagatct tctcagaatt tcgctacgtc gttacgtcct
     8881 ggtcggcttt cagttggatg atgatagcct tccactcttg tctgcaacga accctgtgtt
     8941 caacggctag ctccctgcgt aataggttcc gatctagctc cgttaagcta ccgttagccc
     9001 tcagctcgga gagggtggca aggtaatcct ggcgcaccaa ctccagccct gccaggacgt
     9061 cgtggaattt cgtgagcatt tccataagct cctccaagca ctctggtcgg ggttacggca
     9121 cccgccgttc cgcgcagcgc tccccttgga gagaaccgaa cgaagatagc taagctttga
     9181 ccatcataca ccgattggca acggacgaat ctggcgagag caccctaggc agacggcgaa
     9241 accttgacaa atataccgtc cagacggtat agtaatgaca tgactactga agggcgtaat
     9301 acacggacga ggttattgga ggctgcggag agggtggtgg cgcgtgatgg cgtggcgagg
     9361 ctgaccctcg acgctgtggc cgggaaggcg gacgtgagca agggtgggtt gctctaccac
     9421 ttcccaaaca aggatgcgct catctctgca atggttttgc gatgggtgga tggtttcgag
     9481 cgtgacatcg gccagcggct ggaggtggag gaagctggcg ctgcggggag ctgggcgcgg
     9541 gcgtatgccg gggcaacgtt cgagccggga gagacgcagg aaacgcagcc tcgggagctg
     9601 gtcgctctgc tggctgccat ggcgactgat ccggcgctgc tggagccact gaggagacgc
     9661 tttgaggcgt ggcaggcgcg agcggaggat gatgggttgg acctagcgct ggcgacgctg
     9721 ttgcgtctgg ccgccgacgg gctatggttt gcggacctgt tcgggctggc tccgcctgca
     9781 ggggaattgc gcgggcgggt gctcagggag atcaacgggc tggcgcgagg agaggagggc
     9841 tgatgagctg gttgctgctg gtagtgtcca tcgtgctgga agtgttgggt acggtgagca
     9901 tgaagctctc ggagggcttt agcaggcccc tgtactcggc gctcgtggcg ctcttctacg
     9961 ctctgagcat tgcggggctg gcgctcgtca tcaagcacat agacgtgagc gtggcctacg
    10021 cggtgtggtc ggggttgggc acggcgttca tcgcgatggc gggggtgagc gtcttcggcg
    10081 aggcatttac cgtggtcaag gtcgtcgcac tggtcctcat cgtggtgggg gtggtgatgc
    10141 tcaatatggt cagcccgatg cactagacga tccctatttt ctcaacctcg tagagataca
    10201 ccgatcggat cggcgtcctt aaccgtcgct tccgcgatag cgggaaccca ccgcggagaa
    10261 gcctggagct cccgtacaac ggagcagtag atgcccgcca attgtcttaa acctttagcc
    10321 tctatttgag cccttcacgt cgaaggtcta tactccgttc agttgcagga ctggtagaga
    10381 ggactctggg agattagagc cccacaggat cgctcaacca cgatttctcc cggcgtggtg
    10441 ccgggcactc ttctaaccct aaagcggagg tagatcttga tcggaccgta caccaaggcc
    10501 gtggattccg aggtccagga ggcgctcgaa ggcgaactgg atcgccagcg caccaccatc
    10561 gagctaatcg cgagcgagaa cttcacctcg cccgccgtcc tggcggcaca gggttcggtc
    10621 cttaccaaca agtacgccga gggctatccg ggcaagcgct actacggcgg ctgcgagcag
    10681 gtagacaagg tcgagaacct cgccatagag cgggcgaaaa agctgttcgg cgccgagcat
    10741 gtcaacgtgc agccccactc gggaagccag gccaatgagg ccgcctacgc ggcgctcgtc
    10801 gaccccggcg acaaggttct ttccatggac ctggcccacg gcgggcacct cacgcacggg
    10861 atgaagatca acttctccgg caggacctac gacttcgtcc actacggcgt cggggaggat
    10921 ggctacatcg actacgatca ggtccgagat atcgcgctga gcgagaggcc gaaaatgatc
    10981 ctggccgggg ccagcgccta cccgcgcatc ttcgacttcg agaagttccg tgagatctcc
    11041 gacgaggtcg gggccttctt cctgacggac atggcccaca tcgcaggcct catcgccgga
    11101 ggcgtccacc cttcgcccat accctactcc gagatcgtaa ccacaaccac ccacaagacg
    11161 ctgcggggtg cccgtggggg gatgatcctc tgccgcgaag agttcgccaa gaaagtcaat
    11221 agccgcgtct tcccgggcat gcaaggtggc ccactgatgc acgcgatcgc cggcaaggcc
    11281 gtcgccctgg gagaagcgct gcagcccgag ttcgaagact acgcccgcca gatcgtcaag
    11341 aattgcaggg cactggccga ggggcttatg gaagggggcc tggatctcgt ctccgggggg
    11401 acggacaacc acctgatcct ggtcgacttg aaagataccg gcgtcacagg taaggaactg
    11461 gaagattccc tggaagccgt cggcgtcacc tgcaacaaga acatggtccc gaacgacccc
    11521 gagccaccga ccgtgaccag cggcatcagg ctgggcaccg cggcaatgac gacccgcggc
    11581 atgggtgaag acgagatgca ggagatcgcc gacattatcg tcgccaccgc gcgcggagag
    11641 accgacggcc tgcgcgacag agcaaaagcc cttacggaag cctacccact atatgagtga
    11701 gaccaggccc gacgtaaaga atctccacgt cgtagacggt cccttgagcc gccacaagct
    11761 cgcggtcctc cgcgacgagc gaacctcgac tgccgatttc aggcaggcta tgaaggagct
    11821 cgccctgatc atggtcgccg aggccacacg gtatatgccc acccgccggg tccggatccg
    11881 tactcccctg accgagacgg aggtagagga gatctccggt cccgtctgcc tcgtgccggt
    11941 cctgcgcgcc ggcctcggta tgctcgacgg ggcgctagac ctcctgccca cagcgacggt
    12001 cggctttatg ggcctccagc gcgatgaaga gacggcccgg cctatcgagt actacgtcaa
    12061 cctcccccgc aacctggacg agtacctggt cctcgtcctc gacccgatgc tcgcgaccgg
    12121 aggaagcctc tcggccacca taggcaagct caaggaagag ggggcgacct ggatctcctg
    12181 cctccacgcg gtcgccgccg tgccaggcgt cgagcgtgtt tccaacgagc atcccgacgt
    12241 gaccttctac gcagcagccg tcgaccccga gctggacgac tgctcgttca tcgtcccggg
    12301 cctgggcgac gccggagaca gactctatgg aaccctttga agacccggcg gcagacacct
    12361 cgaactccca cgaagccctg aaaagagtcc ggccttcctg ggacgagtac ttcatgcgca
    12421 tagcccacga ggtcgccacc cgttcgacat gcccgcggct cgccgtagga gccgttatcg
    12481 tgcgcgacaa acgcatcctg accaccggct acaacggcag cccttcagga atgccccact
    12541 gcgacgaagt ggggtgcctc atccggattg tggacggccg ggagagttgc cagcgcacgc
    12601 tccacgccga gcagaacgcc atcatccagg ccgcctacca cggtgtctcc gtcacaggcg
    12661 cctccatcta ctgcacccac caaccatgcc tgatgtgcgt gaagatgacc atgaacgccg
    12721 gtatcgagga ggtccactac gcaggcggct acccagaccc tctcgccatg gagctcgcgg
    12781 ccgaaggcgg cctggccatg gtcaggttcg cctgacgcct taaccgcaat tgggcgggtc
    12841 cgcacaagga cctatccttg ccatggcgtg ggcgggtttc aaacccgccc gaacaacgca
    12901 ccgcccaata caacgaaccg tccgaataac gaacaaccca acacaacgaa ccgtccgata
    12961 caatgcacca ccttacccat acaacgtcct gacccagatc gcctccaacg tacggaatac
    13021 caacacgctc tcttcgcccg aaggccggcg ctcgggatgc ccgaaggcat ctaacaggta
    13081 acgctcggtc atgagcgcca gcgcccgagc cgtctcttcg gggtcgaggt cagcagatga
    13141 cagaccggcc tccacgtccc gcgaaatacg gaccgccacc gcaccgacga ggcgttcgat
    13201 cagaccgtac ctgtaaacct gctcaaccct ctcatcatag cccgccgcat cagcgatcgc
    13261 tcgcaataca ggcccgtact cgacgaaggt ctgtgacccc gccctcaacg cctcgacgag
    13321 aacttccttc gcttcttccc cgacctcccc accagagagc cagcgccagt ccagcgcgaa
    13381 cagcagaccc ccgatgcctt ccaacagccg cgtcaccacc tcatagcggt cgcggaaata
    13441 cgcgtagaag gcaggtcgag acagccccgt tcgaatcatt accccctcga tggtcatctc
    13501 cctgaaaggg tgctcctcca gaaacgcccg cgccgcagcc agtatcgccc gctccgcctc
    13561 ctcaggcttg cgtcgcctgc gcggaaccct atccctgctc acctcatctc cactctctaa
    13621 cctactctcc tctccaatga accacaatgt accgctttat tgacaccgtg tctattgacg
    13681 ttatgtcaat agcaagatat catctgggta gagagtcgcg agaggagtga tccgtggacc
    13741 gtagcgaagc ttggaaggtt cggctggaac ggcggcaacc cggctcgggc ggtctgcggt
    13801 ggtttgcagg agggggcgtt ttccggagat tgacactgat tgcaacagtt tggcccgtaa
    13861 ggtatagttc cggtgatgcc cgacaccaga tctcaaagga accaggcgag cggcgagtac
    13921 gctcgtttcg taggtctcgg gtttactttc atggtcatca tcgcggtgtc tacggcgggt
    13981 gggtacctac tcgactggct attcgggacg ctgcctttat tgttgttggt gggtctcctg
    14041 gtcgggtttg gggcggccct cacatacctg ttcgtaacct tgagcagggt agggcgctga
    14101 tgagcgcgca ctggcggctg gctctgggcg gggcggcggc tacggcggtg atgtctgtag
    14161 gggcagcggc gatcgtcgga tactacttcg gcgccgtcta tgccggggcg tttctgtacg
    14221 gggccggggt cgggctggtg agttttacgt ccatcgcgat cacggcctgg ttgctcggcg
    14281 gcaagatgtc gggggagagg gttctgctgg gagtcgcggt ctacttcgga cggctcgtgt
    14341 tcgcggtggt ggcggtcggg gcgcccatct ttctggcgtc atggccggcg ctcgccctga
    14401 tcggcgggtt cgccggggtc tacgtcgtgg agaacgtgtt gctcttgatc ggggctgtaa
    14461 aggttcgggg cgcgtacggc cgcggggcgg gcgtagggga taggaacggg gggtaggagg
    14521 tttagtgacg ctcgcgttgt tgcaggtgca gggtgcggag ttgcggaagg agatcatcca
    14581 cgcctgggag tcggggcagc acgccatagt gcacctctcg ctgttcggca tagacatctc
    14641 cataaccaaa gccgtcgtgt tcctgtggat cggcgctgct ctgacgttcc tgatcatgtt
    14701 catcggggcc cgcacactga aggaacgtcc cggtgcttac caggtgatcg tggaggagat
    14761 ctacgccttc gggcgcaaca gcatgggtgg ccagctgggc gaagagggga gaaagtggtt
    14821 cccgtacacc ctgagcctgt tcatcttctt gctggtcttg aatctgctcg ggctcgtgcc
    14881 caacagcttc cccgtcacga gtagcatctc cttcaccctg gttctggccc tgcttacgtt
    14941 cataatcacc caggtggtgg gaatcaggcg taatgggttc ggaggatacg tgaaagcgtt
    15001 cgtgccgccg gggatgccgg ccaagccgat catggtccct ttcatgttct ttatcgagtg
    15061 ggtgtcggag ctctccaagc cgctcacgct cgctatgcgg ctctacgcga acatcctggc
    15121 cgggcacttg ctcatcttta tcttcctcgg ctttatcctg tacacgggga cgccgctaat
    15181 ggccgcagta tcggtgcctg tggcggtagt gttgttcggg ttcgagatct tcgtagccgt
    15241 gttgcaagca tacatattcg ctattcttac gcaggtttat atcgagatag ccctgtaccg
    15301 gcaggagcac tagggctccg gcggggcgat aacagagagg agcaatgtaa atggagatac
    15361 tggcattcat cccgccgctg ctggcgctac ttcaggggag cagccagctc tacctcgtcg
    15421 ggttcgggtt cgcggcgggg acggcggtaa tcgggtcggg tatcgggatg gggtacctgt
    15481 tcgggcagac catcgcctcg atccaccgcc agccagaagc gttcgaacta tccaggacgt
    15541 ggctcttcgt cggcatcggc ctcgtcgaag cgcaggcgct gttcgggctg gcattcgcgt
    15601 tgctcatagg gcttcagatc ttctaggtcg aggatcttag gatgagtatc tagggagaag
    15661 catggacgag acaggaatct tcgacatcct gaatacgagc ctgatcttct gggaggttat
    15721 agccttcttt atcttgctgt tcttgctcgt caggtatgtc tacccgccga tccgcgagca
    15781 gatccaggag cgccagtccg agatcgaacg cgccatcgac gaggcggaga agacgcgcgc
    15841 cgaggcccgc gagctcctgg ccgagtaccg gcgccagatc gaggaggccc gcggcgaggg
    15901 ccggcagatc ctcgaagagg cgcgcaagca ggcggaagcc cagcgcgagc ggaccaagag
    15961 ggaggcccgc gaggagggcg accgcataat ccagcgcgcc cgcgaggaga tagaccgcga
    16021 gcgggagtcg gctttgcgcg aggtccgtcg cgaggtcgca gatatggtgg tccaggcctc
    16081 agagcaggtc ataggccgct ccatagaccg tgacgagcac gaacgcctga tctcccaggc
    16141 cctcgacgat ctcgaagccg agatggcaga gagcgggacc gcttgagcgc cgtctccacg
    16201 tacgccggag ccctcttcga ggcggcgcgt gagagaagcg agctcgagga agtactctcg
    16261 gacctcggcg agttcgtctc ggccctgcac gagagcgagg agctaaggct tttcttctac
    16321 ggcgggcagg tacccgagcg ccagaagcgc agggccatcg acggcctgac cgaaggcatg
    16381 aagacctcga gcaccaattt cctgaaggtc ctcgtggaca acgggcgcga ggagatcctg
    16441 gaggaggtgc tcggccgcta tgagcaactc gtcaaagaac acctcggcag gatcgaggtc
    16501 ggggtgacga ccgccgtcga gctctcggaa gaacaggcag gccgactcaa agagcggctc
    16561 ggaagggtgc tcgaaggcag agaggttatt ctggagacga acgttgatcc cgagcttatc
    16621 gggggcgcgg tcttccggtt cgggggacgc atgatggacg gcagtatccg ggggcgcctg
    16681 gagagcctcc gggaggggat gctcgagagg agtgttgttt agtgggcagg ctcaggccgg
    16741 aggagatctc ctccatactc aaaggcgcta tcacagatta cgagaaccgg gtaaggaccg
    16801 aagaggtcgg acaggtcctc gaggtcgggg acggcatcgc gcgcgtccac ggccttacca
    16861 acgtcatgta ccaggagatg ctggagttcg aggacacgcg cggagagaag atcaccggcc
    16921 tcgcgctcaa cctggaggag gacaacgtag gcgttatcgt cgccggcgac gatacgcaca
    16981 tccaggaggg caacacggtg aggcgcacgg agcggctggc cagcgtgccc gtcggcgagg
    17041 gcgtgctcgg ccgggtcatc gacgccctcg ggcgtccgct cgacgggaag ggtgagatcg
    17101 aggccgaaga gacccttcct gtggagatag tggcgccggg catcatcagg cgccagccgg
    17161 tcaagcagcc actccagacc gggatcaaag ccatagactc catgatcccg atcggacgcg
    17221 gtcagcggga gctggtcatc ggcgacagga agacgggcaa gacctcgatc ctgctcgaca
    17281 ctatcatcaa ccaggccgac caggacgtga agtgcgtcta cgtcgccatc ggccagaagg
    17341 actccaccgt agcgggcgtc gtcgccgccc tggaggagtc gggcgcgctg gagtacacca
    17401 ccgtggtgaa cgcctctgcc gcccagtccg cgacgctgca gtatctggcc ccttacgcgg
    17461 ggtgcgctat cggcgagttc ttcatgaacc gtggcgagga cgcgctcctg atctacgacg
    17521 acctctccaa acacgccgtc gcctacaggc agatgatgct gctcctcagg cgcccgccgg
    17581 gccgcgaggc gtaccctggg gacgtcttct acctccactc tcgcttgctc gagcgcgcgg
    17641 cgaagctctc ggaggattcg ggaggcgggt ctctcacggc tctgccggtt atcgagacca
    17701 aggcggggga catctcggct tacatcccga cgaacgtcat ctccatcacg gacggtcaga
    17761 tcttcctgga ctccgacctc ttcaactccg gccagcgtcc cgccatagat acgggcaact
    17821 cggtgagccg cgtggggtcc tcggcccaga taaaagctat gaagagcgtc gcagggaccc
    17881 tcaggctcga cctcaaccag taccgcgagc tcgccagctt cgcccagttc ggcagcgacc
    17941 tggacagggc cacgcagcag accctgaacc gtggggaacg cctcacggcg atcctcaggc
    18001 aggaagagct cgacccgatg ccggtcgagg agcaggtagt cgtcctcttt gccgcgacga
    18061 atggctacct ggacgaggtc gatgccgacg atgtcatgga ctgggagcgg cagttccgcg
    18121 actttatgcg cgacgcccac ggcgacctgc tcgactccat ccgcaccgag caagagctct
    18181 ccgatgagaa cgaggagaag ctgcgcgagg ccgtagagca cttcgactcc aacttcgagc
    18241 ccgagcagac tagcctcgtc gacatctccg tagaggatga gaacggggag gaaggagacc
    18301 agacccgaga ggaagagggg agctagagct tgccttcagc cagggactta cagaggcaga
    18361 tcaacaccgt aaagaaccag gcgagggtct tctccgccct gcagacggtc tcctcggtga
    18421 agttccgcaa ggctgaggcc agggtcaagc gcgcacgtcc ctacgccgag aacgtggagc
    18481 agatgatgcg ggacgtggcg gccaacgcca cgggcgacct ccccctccta acagggcggg
    18541 aagtaaatcg cgtcgccgtc tgtactctca ccgcggacag gggcctggca ggcggtttca
    18601 acgcgcaggt gctccgtagg accgtccgcc tcagggagga gaaagactcg gatgcgctgc
    18661 aggtcgcgag cggacgcaag gccgtcgcgt tcttccggtt caggcgcgtc gagctcgccg
    18721 aagtcttcac cggtttcacg gacaacccga cctacgagaa cgcccgcgag atcggacgca
    18781 cccttactcg catcttcgat gacgaagacg cggacgaggt ctatctcgtc tacaaccgtt
    18841 tcgagagcgc cctcaaccag agacccgtag ttaaccgcct cctgcccgta gccagggagg
    18901 aggacgaaga agaggatagc aataactcct acatggacta cgtccccgac gcggagacgg
    18961 tattgaagcg gctggtaccc cgttacgtgg agaccatggt gtggcaggcg ctcctggaag
    19021 gagcggcagg cgaacacggc gcccgcatga cggcgatgaa gaacgccacg gacaacgcaa
    19081 acgacctggt gcgcgacctc accctccaga tgaacaaggc gcgccaggcc cagatcacgc
    19141 aggagatact cgagatcgtc ggcggcgcgg aggccctctc cgcgggctag agaacaggag
    19201 tttttatgag cgagagcgag agtgggcaga ccttcaaccg cacgaccttc ggcggtgaga
    19261 ggggcgaggt agataacggc cacgcggcca ccgagcagga gagcggcagg gacggccacg
    19321 aagagcgggg cgagggagtc ggcaccgtcg tcgaggtgaa gggacccgtc gttgacgtca
    19381 ggttcccgcc cgaagacata ccggagatct tcaacgctgt tacggtgccc atcgagaagg
    19441 acggggagac catacagttg accctggagg tgcagcagct actcggggat gacatggtcc
    19501 gcacggtggc catggcctcg accgacggcc tgaggcgcgg ggtcgacgcc agggatacgg
    19561 gccgccccat agcggtgccc gtgggcaaga acgtgctggg ccgcgtgctc gacgtgctgg
    19621 gtcaacccgt cgacgagcag ggaccggtgg aggaccccga tgactactgg cccatacacc
    19681 gtccttcccc caagttcgag gacctcacca cccaggccga gcagttcgtc accggcatca
    19741 aggtcataga cctcctttgc cccgtgcgca ggggcggcaa ggtaggcctc ttcggcggcg
    19801 cgggcgtggg caagacggtc gttatacagg agctgatcaa caacatagcc ctgcagtacg
    19861 agggcacttc ggtcttcgcc ggcgtcggcg agcgcacgcg cgaggggaac gacctctacg
    19921 gagagttcga ggaggccggg atcctctcca acgtgggact ggtcttcgga cagatgaacg
    19981 agccgcccgg cgcccgattc cgcgtcgggc tcaccggcgt caccctcgcc gagtacttcc
    20041 gcgaggagct ggggcaggac gtgctcttct tcgtggacaa catcttccgc tttgtgcagt
    20101 cgggtaacga ggtctcggcc ctcatgggcc gcatgccctc cgaggtcggc taccaaccca
    20161 ccctcggcac ggagatgggc gcgctccagg agaggatcac atcgacgaag aagggttcga
    20221 tcaccagttt ccaggccgtc tacgtgccgg cggacgactt caccgaccct gcgccattca
    20281 cggtgttcgc ccacctcgac tcgacggtgg agctctcgcg gtccctgtcg gcccagggta
    20341 tctaccccgc cgtcgacccc ctgacctctt cttccaccat cctcgaccct tctgtggtcg
    20401 gcgacgagca ctatcaggtg gcccaggagg tcaagaacat ccttcagcgt tacaaggagc
    20461 tgcaggacat catcgccatc ctcggcatag acgagctctc cgaggaggac aaggtcatcg
    20521 tcggccgagc caggcgcctc cagcgcttcc tctcccagcc cttcttcgtc gccgagcagt
    20581 tcaccggcct cgaaggcaag ttcgtcgagc tggaggacac gattcgctcc ttcaaagagg
    20641 tcgtcgaggg caaccacgac gagctgcccg agcaggcctt ctacctcgtc ggcaacatcg
//
LOCUS       DATMVF010000126        43269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_342247, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000126 DATMVF010000000
VERSION     DATMVF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 43269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43269
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342247"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="VN178_06300"
     CDS             complement(<1..1617)
                     /locus_tag="VN178_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HWS80619.1"
                     /translation="MFTTRPELVGTHGMVASTHWLASAAGMAVLESGGNAFDAAVAAG
                     LTLQVVEPHLNGPGGEVPIIFHSAERAQTLVIGGQGPAPAAASIAHFEELGLELVPGT
                     GLLAACVPGAFDAWMRLLGEFGTLRVSEVMRYAIDYAGHGYPLVPKISDTIGRVEKLF
                     RDEWTSSARVYLRGGEIPKPGTLFSNPDLASTLRRIVEEAEARSADRDEQIEAARDAF
                     YRGFVAEAIVRYVTSTRVMDTSGRRHGGLLRGDDFAAYRAELEGPATLDYHGYTVCKT
                     GLWGQGPVFLQQLALLDGFDLVGMGHNTVEYIHTVVECAKLAFADREAHYGDPRFVDV
                     PPEELLDPSYADSRRDLIGEDASFELRPGAASGRSPRLPDPPSAATDQSTGAGEPTVP
                     RDDQGSGDTCHVDVVDRHGNMVSATPSGGWLQSSPVIPGLGFPLGTRAQMFWLTEGLP
                     NSMAGGKRPRTTLTPSLALRGGEPYLAFGTPGGDQQDQWSLNFFLAHVHFGLNLQEAI
                     DAPNFHTEHFPSSFYPRTAAPGKIVVEDRLE"
     gene            complement(1627..2616)
                     /locus_tag="VN178_06305"
     CDS             complement(1627..2616)
                     /locus_tag="VN178_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS80620.1"
                     /translation="MTEQTQNGEANGRPLLEIRDLKKYFRVGRENLKAVDGISLEINK
                     GETFGLVGESGCGKSTAGRVLVRLYEPSGGEVLFEGKSIHEATGSEGRALNRKMQMVF
                     QDPQASLNPRMIVSDIIAEGIDIHNLAANGKERLDRVHELLETVGLNPEHANRYPHEF
                     SGGQRQRIGVARALAVDPDFIVADEPVSALDVSIQAQVINLLKKLQRERGLTYLFISH
                     DLSVVKYISDRVGVMYLGVMVEMAPSDELYSNPLHPYTQALLSAVPVPDPDVELERER
                     IVLQGDVPSPVNPPSGCRFRTRCPYATEACGHDEPVWREVSPEHWVAACHCIP"
     gene            complement(2613..3656)
                     /locus_tag="VN178_06310"
     CDS             complement(2613..3656)
                     /locus_tag="VN178_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS80621.1"
                     /translation="MVNESTNGTSDAVLRVDDLRISFSTYAGEVQAVRGASFDLRRGE
                     TLAIVGESGSGKSVAARSIMRLNPAANTIVRGGEIVFEGEDLLRLSERRMLEIRGPKI
                     AMVFQDPMTSLDPTMRIGRQISESLRKHLGMSRAQAAERAVELLELVGIPNAEERLRQ
                     YPHQFSGGMRQRVVIAIALSCDPQILIADEPTTALDVTIQAQILELMRDLQERLGMSI
                     ILITHDLGVVASTAHRIGVMYGGKIVETGTVREIFYNPQMPYTWGLLASIPLPTADRS
                     QELIPIPGSPPNALDPPAGCPFSARCPYAMRICEEEMPGYTTFSDEHRAACWLHHRMA
                     PKVEPPASIGGAK"
     gene            complement(3658..4590)
                     /locus_tag="VN178_06315"
     CDS             complement(3658..4590)
                     /locus_tag="VN178_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80622.1"
                     /translation="METRDRELTPEQFRALEEGSRDSERIAGPSIGFWRDSWERLKKN
                     RAALVSLALIVILFAFAFVLGPLLTPFSPSEQDIAQRYERPGAEHWFGTDEFGRDMFA
                     RVWQGMRVSLYIAFIAAGLDLVVGVTFGAVSGLLGGKVDGVMQRFIEILNGIPSLVVA
                     ILAMVVLDPGILSISIAIGAAGWTPMARLIRGRVLQLKDQEFTLASRSLGAGNRRLLT
                     KHLLPNALGLIIITLMLTIPSAIFTEAVLSFIGLGIQVPNASLGSLIDAGAEDMRFYA
                     YLLFFPAAVFCLLMLSFNLLADGLRDALDPKMRK"
     gene            complement(4594..5529)
                     /locus_tag="VN178_06320"
     CDS             complement(4594..5529)
                     /locus_tag="VN178_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80623.1"
                     /translation="MLRFVGRRLIIMFITLYLIISLTWVLSKALPGTPFADEKLTPSS
                     RAQLFEKYGLDDPLPVQYVRYMLNVAQGDLGNSFYFESRPVTQIILEQAPVSAFIGVQ
                     AIIFGLIPGLALGVLAALRHNGVLDYLGTTVAVAGIAVPSFVLGPILQYWLGFRWELL
                     PIAWDPFAPAWTMSIMPSISLAVFVTAIVARYVRAEMLEVLGQDYITLAKSKGLSWQA
                     VVWRHVLRNSLIPLVTVLFPLMVGIITGSIVIEQIFAVPGIGDLFVRSIFVNDYATIL
                     GTTIFFSVLFILAYLIQDILYAVIDPRIRVAGGEE"
     gene            complement(5651..7342)
                     /locus_tag="VN178_06325"
     CDS             complement(5651..7342)
                     /locus_tag="VN178_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWS80624.1"
                     /translation="MAIRRVRPGKALVGTQFSRRDFLKISGSGLAGAALLGTAGCGIF
                     SGGGGGGGGTGGGGGGGSSITLNLQATIRDLDSTTTTDSVSSGILINVMEGLYRLDEN
                     LDPQPAMAEGVQISDDKLTYTFTLRDGVKWSNGDPVTSKDFEYAWLRGLDPDTAGQYA
                     YILYTFIEGAEEFNSGDGSADGVAIEAPDDKTLEVTLVSPSPFFLGLTAFTTYLPQKQ
                     SFVEEQGDQYGQSADALLYNGPYSLTGFNPTQGVTYVKRDDYWDAGNVDIQRIEGRIV
                     TEIGTAVNLHEAGNLDVTEISQEYVNQYRDSPDFYSQSNFATFYLVFNNDLPLFQNEN
                     IRRAFQMGFDQQTLTQEILQDGSEPGVGLVPNGIDGPGSQTFREAEGPTMPEFDLQEA
                     KRLFQQGVEEIGENPTIELLSYDTTGGRDVATFLQEQFEKMGAKINVTVQPFDRKLEL
                     EADGEFQLSFQGWIADYNDPMTFLDLFLSDSSFNTGKYKSERYDELINQALTETDFAR
                     RMNQMLEAERVLIEEDAGLAPVYFEGQVWLIEPSIKNFVYYRSGGDAIKLWRLQS"
     gene            complement(7638..8576)
                     /locus_tag="VN178_06330"
     CDS             complement(7638..8576)
                     /locus_tag="VN178_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80625.1"
                     /translation="MERGDRQITLTQDLFEPVKRSVRDAEELSAPPAGFWSDAWRRLK
                     RDRIALLAGGIIFLVFILAVIGPWLTPYTPFAQDLERQYREPSLSGFWFGTDEFGRSM
                     FDRVWVGTRVSLYIALLATALDISIGMSYGAISGYYGGGVDNVMQRIIEVLVGIPNLV
                     VIILMMLILEPGIIAITLALAITSWPSSARLIRGQVLRLKEQEFFLASRSLGAGTFRL
                     IVKHLIPNVFYIVIITLMFTVPSAIFFEAFLSFIGLGIQPPNASLGALISDGADQMRF
                     YPYLLIFPAIVLALITVSFRLLGDGLRDALDPRMRM"
     gene            complement(8585..9511)
                     /locus_tag="VN178_06335"
     CDS             complement(8585..9511)
                     /locus_tag="VN178_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80626.1"
                     /translation="MIGYTLKRLVNACVILFVIATATWFLMQLLPGSPFNDQKLSPEA
                     RAQIEAKYGLDDPLLVQYVRYIGNLAQGDLGNSYQYSGKPVLEIILERLPVSAFLGAQ
                     AVVLGVIGGLLLGVIGALKHNSLADSGTALLAVLGIAVPEFVLAPLAQYWLAFKLGIF
                     PPAFFESWWHSVLPSMVLAITVAAVVATFTRTEMLEVLGQDYVTLAKSKGLNKVAVVA
                     RHVLRNSMIPLITIIVPLTAALVTGTLIVETVFAIPGIGEQFVRSIQVNDYSMIIGTT
                     MLFSVFFVASYLIQDILYGIIDPRIRVVGHGD"
     gene            complement(9538..11232)
                     /locus_tag="VN178_06340"
     CDS             complement(9538..11232)
                     /locus_tag="VN178_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWS80627.1"
                     /translation="MAIRRVSPGKALVSTQLSRRDFLKISGSGLAGAALLGTAGCGVF
                     ENSGGTGGGGGGGNVLNVNLATEIRYLDSALATDEISFGILVNTQEGLYRLDPDQKPV
                     PAAADGAEVSDDGLTYTFTLRDGIEWSNGDPVTSRDYKYAWLKVLNPDTASEYSYIIY
                     TFVQGAAEYNAGDGSAGDVAIETPDDRTLRVTLVNKAPYFLQLTSFPLYFPQQQEYVE
                     QQGDDYAQDADTLIYNGPYVMTQGGVGAGGTTVLEKNDRYWDKANVAVQRINGRIVKD
                     SDTALNLYEAGELDITGLVGDKVARYEDSPEFYRRVEPTTFYGQVNHDVPALANLNIR
                     KALMTGFDRKTLTERVLADGSEPAYALVPPAITPGPGDQTFRQALGDLLPTDTSGARQ
                     LWEQGVEELGETPRLKMLFDDSSIGRDIATFIQGQYKENLGADLEIEITTFEAGLTRV
                     QERDYQVSFVSGWGADYNDPMTFLDLFLSDSPQNTTGWSSERYDELINGAKREGDQER
                     RMQMMAEAEPILFDEVVLIPEYYSAVAGLKKPYFKGFAPHDFGGSPDFKYASIEGK"
     gene            complement(11481..12527)
                     /locus_tag="VN178_06345"
     CDS             complement(11481..12527)
                     /locus_tag="VN178_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWS80628.1"
                     /translation="MNPSLPSSIRVDALPLLTFSVVILGLVVLLPFPAHAAEERLVGD
                     VVVGHGGVEHDVSTGFGDLEVRGLARDDVHSGFGDILVTGRVEGDIDAGFGNVKLEGP
                     VEGDVRAEFGDVYVNDSVEGDLNVGWGDVELGPDAEISGDLECEGCEITGNRSAVDGH
                     MTARGMALDFDESHGPGVVGFFGWLFMALAFAACAVLAAVVAPRPMASAAGRAEESPG
                     RSFVYGLLSLPVVFVLCVVLAVSIVGIPLLLLLVPAYLALLFFGALVAAFFIGTRVLM
                     ITGRYRVGNALAAVVGAMILAATTFIPVLGNLLLYALSLLGTGAIILALFSRRRPRAS
                     HPSYEAYVRDRAGG"
     gene            complement(12524..13024)
                     /locus_tag="VN178_06350"
     CDS             complement(12524..13024)
                     /locus_tag="VN178_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWS80629.1"
                     /translation="MRRPLPCDPESVFELADGSLGPEREREIRAHLRDCGGCLSLYER
                     ELLLNASLGSLEFEETNSVCRSVAMALPTRSSKARFLWSVLAVVLLLTTSVAMILFGT
                     NLAVFVVDALNVFWGFVAGLADVIQVVFAAIGKALLIALAVGAFVDLVIAAAYLAVYR
                     RRAREA"
     gene            complement(13021..13614)
                     /locus_tag="VN178_06355"
     CDS             complement(13021..13614)
                     /locus_tag="VN178_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS80630.1"
                     /translation="MTLGGWREKLGSLSVRREELDDKELVARTLSGDLRSYEELVGRY
                     ERLVGRVVYPYARREISVEDLVQETFLRAYDRLETFNPDYRFKTWLLAIANNLGVDTL
                     RRRREIVEFNPEAHAPVSESPESDAVEADRARSVKEAIATLPEAYGVPLILRYTEDLS
                     YAEIAEVLSISVPAVKSRLFRARNMIAGKLEEAGERE"
     gene            complement(13791..14519)
                     /locus_tag="VN178_06360"
     CDS             complement(13791..14519)
                     /locus_tag="VN178_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWS80631.1"
                     /translation="MLTSHSTGGFATTSAPEGFDLQDFEEASLRTVERRFGPRDTIFT
                     PGDPDDQLYFLLSGTVRLYKIYGDYKEATTALLKEGGVFGKLNLVEGRWQDVFAEAVT
                     EARVASIQKASIEQVIKSDPGFALKLFSSFSERLRQSDEVIESLLHREVSTRLATLLI
                     NLGERFGERDGAGTQIDVRLTHQDLANMIASTREAVSKVMSELQRDGIIESRNRRIII
                     VNGEALAEHASRLSGLSIDGQAAI"
     gene            complement(14650..15270)
                     /locus_tag="VN178_06365"
     CDS             complement(14650..15270)
                     /locus_tag="VN178_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80632.1"
                     /translation="MEEISRITKRRRRLRTVGEPHPWEPRHMRLTAMRFAESVGEPAE
                     RRNVTLGVIQHLWRSPVPFIGFYPPEGVRVTQNRLYTPAPPRSVDDTDYLAGVEAHKR
                     AFAKDLQNAMIPAGSLPGYFEVPTDEGWRITLELAEGRLRLADAWGMNSERRVAGDLA
                     PFAWAYRNCDFCIVTIYDKDIGALRDDLLFVAKRAGVELRWRRSAA"
     gene            complement(15376..16077)
                     /locus_tag="VN178_06370"
     CDS             complement(15376..16077)
                     /locus_tag="VN178_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HWS80633.1"
                     /translation="MFIPGGFGGYLLIMIVGMLISGAAALWVRSSYSKYSKQRSASGL
                     TGAQTARMILDRNGLTNVRVEPVAGHLTDHYDPRSKVIRLSEDNFRGNSIAGVSVAAH
                     EVGHAIQDASGYVPMKLRAGIFPIVNFSSQIWPLAFIMAFFVGGAASPFGSLLINVAI
                     VLFLGVLLFHVVTLPVEINASTRAYGLLTRYGILSGTEAGGTRRVLTAAAFTYIAAAL
                     TAVLTFLYLLAVSRQ"
     gene            complement(16360..18288)
                     /gene="acs"
                     /locus_tag="VN178_06375"
     CDS             complement(16360..18288)
                     /gene="acs"
                     /locus_tag="VN178_06375"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWS80634.1"
                     /translation="MAEETKVRTYEPPEEFAREANVDDASVYEEAERDYEGFWAERAR
                     ELHWFKEWDRVLDWDPPEAQWFVGGKLNVAYNCLDYQIEQGRGDKTAILWQGDEPEDS
                     KAFTYSELKAEVERFANVLKDLGVQKGDPVAIYLPMIPELPIAMLACARIGAPHSVVF
                     GAFSADSLRDRINDCEAKVLVTADGGPRGGKVTPLKANADEALEDTPSIESVVVAQRS
                     GEDVNMVEGRDRYWNDLMGEADAECPAEEMDSEDILYILYSSGSTGKPKGIVHTTGGY
                     LTQVKATTRWVLDIKEDDVYWCQADIGWVTGHSYLVYGPLSNGATTVQFEGTPSYPGP
                     DRHWDVIERNGVTIYYSAPTVIRAFMKQGTEPIEKHDLSSLRLLGTVGEPINPRAWEW
                     YHENIGGGRCPVVDTWWQTETGGIMIAPLPGLTPTKPGSATRPFPGIFVDIYDEDDNP
                     IEGAGKGNLVVTRPWPGMLRTIYKDPERFRETYWERLGDKYFVEDGAERDEDGYYTIT
                     GRIDDVMNVSGHRIGTMEVESALVSHTHVAESAVIGRFDEDKGQAIVAYVILEGDREG
                     TEELIKELHDQVRKAIGPHARPEEVIFTPDLPKTRSGKIMRRVLRGVAEGEDDLGDTS
                     TLADPSVVDDLKDARSQS"
     gene            18561..19340
                     /gene="fabI"
                     /locus_tag="VN178_06380"
     CDS             18561..19340
                     /gene="fabI"
                     /locus_tag="VN178_06380"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HWS80635.1"
                     /translation="MGGLLEGKKVLVTGVLDRRSIAYSAARLAQEQGAEIVLSGFGRA
                     RSLTERTARRLDPEPPILELDVNKPEDIEAVADDLGARWGELDGIVHAIAFAPEDALG
                     GNFLTAQWPSVATALQTSAYSLKALSVGLVDLLGPGASVVGLDFDARIAWPAYDWMGV
                     AKAALESTSRYLARDLGGRNVRVNLVSAGPVKTLAAKGISGFEEIEGEWTRQAPLGWD
                     TTDPEPVGRAVVSLLSDWWPATTGELIHVDGGYHAMGAPIK"
     gene            complement(19470..20066)
                     /locus_tag="VN178_06385"
     CDS             complement(19470..20066)
                     /locus_tag="VN178_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80636.1"
                     /translation="MRRAVILATLTCILFAVAGVTVAGENRFTSGSQDGDQTESTVAG
                     RTVPEATAREATVPEATVPETTVSEDVEKPVEEAEETTVVVRKEPKGPGKGDPGAGSY
                     GGIREDDSFEKAGGPTGKPGKPEHVRGGKKYGKSRVAGEPPGKSKPAKDGPDHAGKKG
                     IGGNPGKVTLCHKNRVTISVGAPAEPAHLRHGDELGAC"
     gene            complement(20146..24045)
                     /gene="dnaB"
                     /locus_tag="VN178_06390"
     CDS             complement(20146..24045)
                     /gene="dnaB"
                     /locus_tag="VN178_06390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWS80637.1"
                     /translation="MRSVETGAEAARVPPHDLDAERAVIGAMLVSETAVAVVAERLAA
                     EDFYSEVHRIIYGATMRLYSRGEPIDQLTLTNELRSVNEFERIGGRPYVFQIVESVPT
                     AANAGRYADIVRGKALLRAIIDVGSRITEDAFREPEDVSEALDSAEQLVYGVSNRTLR
                     ERLAPVSELAPGALEMIQRLYEQEGEVTGVETGFEDLDRLTTGFHKSDLIILAARPAM
                     GKCLKWDSLVVDPDSGERLTIQECVERRLPRVLNVSSAGSVRTAAVSDWIDSGVKPCF
                     RVTTRTGRSVEVTGHHPFLTVDGWVPLHDLEVGNYVAVPRRVPIFGSDESWQLDRVRL
                     VAYFIAEGGLTGGCPKWSNADPALVEDFRGAISRQFPKVQVVHDGSRHYDYKLVCSDR
                     CGRFNSNPVTAWLRELGLMGKYAEEKSFPDCVWRWSRRYLAEFLRVLFSCDATIYRLG
                     RYPRIEFTVCSERLARDVHHALTRFGVVAKLWQKKERCWRVEITAPDSVSRYQAEVGW
                     IGEKSLRFVDETFTRRELPRRGSNYGHPPSQVWDVVRRVAAQRSLSLAELARRSGETV
                     KIGKYAGHNPHTGRSLPAYRLRGYAEVLADQRLLQVASQDLLWDEIVTIETIGDHQVY
                     DLTVPDGSNFVAQDICVHNTAMALNAIWHASGEKKMPVAIFSLEMSKEQLVQRLISQI
                     TRIPAQALRSGNVKAEDWPKLVRGVAEVSRAPIWIDDTAGVTLMEIRAKVRRLASQLN
                     VAGEMPLSLVVVDYLQLMVGQGNRAENRQQEIAEISRGLKVLARDLDVPVLAIAQLSR
                     AVEARHDKRPLLSDLRDCVTGDTLVLLADGHRTPVRELVGTRQRVLAMSPVGKIVEAE
                     SDRVWSVGRRPVFEVRLASGRSVRATAKHRLFGADGWIRVSELRPGDRLAIARHVPEP
                     ENAPGWPEARLALLGHLIGDGSYLSGQPLRYCTASEENSRLVAQAAAEEFGVTVNRHA
                     GRGNWHQLVLSGNGNRWRPAGVNLWLRELGIFNQRSHEKRVPVSVFGLDNESIAVLLK
                     HLWATDGTIFARKSGTRGSSTICFSTNSRGLAHDVAALLLRLGIVGRIREVAQGAHRP
                     MFTVSISGAREQMLFLKRVEAFGPRCDGAEKLARQLLIRSAGTNVDTLPIEFFEKVKA
                     SMAARGMSHRAMSLARDTSYGGSSRFRFAPSRTLMAEYAGLLDDKTLLADAESDLFWD
                     RVIEVVPTGEDEVYDLTVPGPASWLADGIVSHNSGAIEQDADMVMFLYRDEYYNSESD
                     DKGIAEVIVGKHRNGPTGKVQLAWMEQYTKFASLARR"
     gene            complement(24210..24659)
                     /gene="rplI"
                     /locus_tag="VN178_06395"
     CDS             complement(24210..24659)
                     /gene="rplI"
                     /locus_tag="VN178_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWS80638.1"
                     /translation="MQVILNQDVEKIGQRGDIVDVSRGYVRNFLVPRGLAEVATPGKL
                     EEARRTMEESEERNRRLAERAEEISNILNKSVITIEARTGEDERLFGSVTAANIADAV
                     ERARGIRLDRRRIRLEEPIKALGTHQVPIQVHGEVEASVKVIVVPKL"
     gene            complement(24665..24916)
                     /gene="rpsR"
                     /locus_tag="VN178_06400"
     CDS             complement(24665..24916)
                     /gene="rpsR"
                     /locus_tag="VN178_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWS80639.1"
                     /translation="MGRRRNGPPRGGRPTKSKPCVFCKENVAYVDYKDYNLLRRFISD
                     RGKVRARRVTGLCPQHQREAASAIKRAREMALLPYVVGR"
     gene            complement(24935..25249)
                     /gene="rpsF"
                     /locus_tag="VN178_06405"
     CDS             complement(24935..25249)
                     /gene="rpsF"
                     /locus_tag="VN178_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWS80640.1"
                     /translation="MLIVIPELDEEQVENTVGRFRTIIERTGGEAGAPNDWGRRKLAY
                     EIDHRTDGFYVVMEFTAGERTLVELKRILRVSDDVLRHMVVKLPPGYAHKPLPEPAEV
                     TA"
     gene            25445..26194
                     /locus_tag="VN178_06410"
     CDS             25445..26194
                     /locus_tag="VN178_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS80641.1"
                     /translation="MENGAGIAVETVGLEKVYGEGPTAVRALQDVSLAFPNGEFAAIM
                     GPSGSGKSTLLHILGALDKPTSGRVVVGGTELSGLSDKKLTLLRRERMGFVFQFFNLI
                     PTLSAEENVLLPVLIAGKRPGDYGERLDELLDLVKLTARRTHRPDELSGGEQQRVAIA
                     RALIRFPDIILADEPTGNLDSKTGAEVLELLRESAARFEQTILMVTHDPRAAATAGRA
                     VFLSDGRVVDEAIDPDQDEILERIKTLEPDG"
     gene            26199..26663
                     /locus_tag="VN178_06415"
     CDS             26199..26663
                     /locus_tag="VN178_06415"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWS80642.1"
                     /translation="MSDYRFRMMEDEEATQISGWHYDPPYDFYDATSDPDDLEELLDP
                     KRRKDAYFSVFDDEGVLVGFFQFERDNKTVDVGLGMRPDLTGRGLGIGYLLAGLKFAR
                     RRYSPERFTLSVATFNERAILVYERAGFRRDTIYKHNTDGGEYLFLSMVKEA"
     gene            26660..29221
                     /locus_tag="VN178_06420"
     CDS             26660..29221
                     /locus_tag="VN178_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80643.1"
                     /translation="MKLRRFSGLSFRNLRARVQRTLLTAVGIVLGVGIVFGVLTLSNT
                     MSGTFAELFTKAYGSADLTVTAAGGGGGFDQKAVEEVRGYEGVESAAPRYALSSSLIL
                     QRNQENDLPEVRSMRLFGVEPQSAKLATGFELTDGHFPRSGKELTLDGGSAKRAGLGI
                     GDRVTVGTPEGPKELRLVGLLRIPGGSFGGLAFGMAPLPFAQNVFDKRGQISGIAVEA
                     EDGVSVSDLGERLDRGLNEGLQAERSETRTQEISGQLQGFKIALLFFAGTSLFVGAFL
                     VFNALSMTILERTRELGMLRALGSTRAMIARSVIVEAMLLGVTGSLLGVLFGYGMARG
                     LVYLFGKAFMFEITSLVLSSFALVSAIVLGIAVTVVAALYPAVRAGRVSPVEAMRSAG
                     AADEAGHSRVVSLLAPVLGLALAGAGIPWIYYLAKNLSASLGGLVYASGIAAIIAAFL
                     GISLVVPVLVRPLAALFSPALRLLFGVEGRMAAANATRNRVRTALTASALMVGISLIV
                     AFSALGGSLLGSIRDYLDGSLGSDYVVQPTQQNSDAGFSAQLPEKIGRVQGVEKTTSI
                     VSTFRREGKKVDAVFGVDQNYPDIFRVDYAAGGPDAFLRLKNGDALVGKQLAKDRKLE
                     VGSRIGLLGPKGEKEYRVEGILENDIVGGGMGIYLSQEILASDFNETQSEFLAIKARS
                     GSDREALARRIEAVLADYPQFTLYSNAEWKEQIESDFNRQYVFFYAIMGVSVAVSAFG
                     VVNTLSMSVFERTREIGILRAVGTTRLQIGRLIIDEGIVISLIGCLIGVALGSLLGYL
                     FVQGSGAGGFEIDFYYPKLPALAALLSGLFIGIFSGLLPARSAARKDIVEAVQYE"
     gene            29482..30999
                     /gene="crtI"
                     /locus_tag="VN178_06425"
     CDS             29482..30999
                     /gene="crtI"
                     /locus_tag="VN178_06425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HWS80644.1"
                     /translation="MDALAASSARPNKRVAVVGAGMGGLAAAIRLAAMGFEVEVFEKN
                     AQPGGRVGRLEADGFSFDTGPSLLLMTDTYRELFRFAGRDLDDYVRLMPLDANYRVTF
                     GDGDALTIRRTLPELIGELERIEPGVTPRFYRFLEDACNKYRLGRSEFVERDFDSARD
                     FFGLRNLRLLLKTKALGNYYRQVSRYFRTEKLRQAFSLQTMYLGLSPFEAPAVYSLLP
                     YTELAEDGLWFPEGGMYSLVGAMERLAAELGARFRLNSQVERVAVTKGRAHGVVVDGE
                     EIEADAVLANADLPYAYRELLAGSADKDFLLRRREKLRYTASAFMLYLGVEEKLGTMV
                     HHNFYLSGRYRENFEAIFRDRTLPEDPSFYAVVPSKTEPGMAPDGMESLFVLVPVPHL
                     GPNVDWDRDEDAFKEKVYGLLERRCGLDRSRVVYEKARTPLDWRDEYNLEEGAAFGIG
                     HGIFQVGYFRPPMVSKAVQGMYFVGASTRPGTGVPLVTIGAKLVADRIGREVGGS"
     gene            30996..31940
                     /locus_tag="VN178_06430"
     CDS             30996..31940
                     /locus_tag="VN178_06430"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HWS80645.1"
                     /translation="MKLDPAGGKKLDESLSLAESYELCRKVQKAHSRTYYFSTRLFPA
                     EVRPRVHALYAFMRYADEIVDHPHDLPPGAQLSVLEEFEAETMAAVSGESVPNPVLRA
                     YADTVFTCGIDPETITAFMESMKMDTRVFRYRTFSDLEEYTYGSAAVVGLMMCRVVGV
                     AEGKADRHAEALGVAMQLSNFLRDVGEDWRRGRVYLPLEDLERFGYTERDLALGVVDE
                     RFVALMRFEIERARRLYEDADEGMGYIPRGRRFPVIVARELYAAILDRIEAHRYDVFC
                     RRAQVSRPAKLLVAAKCAAGDPGEIADRIRYRRTAPAR"
     regulatory      32018..32128
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            32193..34046
                     /locus_tag="VN178_06435"
     CDS             32193..34046
                     /locus_tag="VN178_06435"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HWS80646.1"
                     /translation="MIPNLRELLGTRMLVGDGAVGTLLGERGVGFGHPYARANLSHPE
                     MVTDIHTEYLRAGADVIETNTFAANRFKLENHDLEESVREINAEGARLARMAARNVAG
                     TGDRALVLGAVGPLGRPLAPVGSVGPDGAKSVFVEQAEALLEGGADAILLETFTDLYE
                     LRLAYEAVEALGAPVLAYKTFVEDGETLAEGLPGRAAREISSWGADLAGANCTVGPQR
                     MVEIISQMAGGAGPVAAFPNPGLPQLVGGHIRFQRDVDHFAEYGVKLAEAGARLVGGC
                     CGTTPAHVRALSRALRDFEVAGNGARRPVAVVEQAREKEISAEPVSEFAERLRTGFAV
                     TVEVDLPRGNDIGRVVEAARRLKERGAHAIDISDGARARLRMHPVAAARIVQEEAGIE
                     VVAHISCRDRNIIGLQADLLGAAALDVKNILAVTGDPAQIGDYPEATSVFDTDSVGLV
                     HVLSKMNRGEDLAGNPIGEAPGFLVGASFNPTAEDLDQEVEKLRRKVEAGAHAFWTQP
                     VFEMGALENALERIGDDGVCILLGLMPLRSARQAEFLHHEVPGIYIPEHVRRNLASLP
                     PEDAPRYGVEVAQELLAEAQPLVCGAYIMPPASSPDLAGDVIEAMSLPAGR"
     gene            34053..37838
                     /gene="metH"
                     /locus_tag="VN178_06440"
     CDS             34053..37838
                     /gene="metH"
                     /locus_tag="VN178_06440"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWS80647.1"
                     /translation="MVLQHTKNEERQARLQEFRELLEQRIVLLDCAMGTMIQSYGLSE
                     SDYRGERFADHPGELGGNNDLLSITQPEMIRAIHEAAMDAGSDIIETNTFTSTSIAQA
                     DYQLEDLAYELNYEAARIARETADAYTEKMPERPRFVAGALGPTNRTASLSPDVNNPG
                     FRNITFDELAESYAEAARGLVEGGADLLLVETVFDTLNARAAIFGITSYLEEEGFEVP
                     IMISGTITDASGRTLSGQVTEAFWNSVRHANPISVGLNCALGSKELRQYVEELSRISD
                     VPVSAYPNAGLPNEFGEYEETPELMAAEIHDWARAGWLNIVGGCCGTTPDHLRAIAEA
                     VSGHPPREIPEIPKKLRLSGLEPCNIGPDSLFVNVGERTNVTGSARFKDLISEGDYET
                     ALEVARQQVENGAQIIDVNMDEGMLEGEEAMVRFLDLVASEPDISRVPVMIDSSRWSI
                     IEAGLKRVQGKAVVNSISLKEGEEEFVRQALLLKRYGAAVIVMAFDERGQAGTKERKV
                     EVCKRAYRILTEELRFPPEDIIFDPNVFAIATGIEEHDDYGVDFIEAVREISSTLPHS
                     KTSGGISNVSFSFRGNNPVREAIHAVFLYHAIRAGLTMGIVNAGQLAVYDEIDGDLRE
                     AVEDVVLNRRPDSTERLLALAERYRDRDEAGSDEKDLQWRGWPVEKRLEHALVKGIAG
                     FVEVDTEEARLQASSPLDVIEGPLMEGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAY
                     LIPFIEEEKGGEPRKSNGTLVMATVKGDVHDIGKNIVRVVLQCNNFEVIDLGVMVPSA
                     KILQTAREKGADAIGLSGLITPSLDEMVHNASEMQREGFDIPLLIGGATTSQTHTAVK
                     IAPGYEQPVIHVKDASRAVGVVQNLVSENRRAAYAQQTAAEYEQVRKKHQGRRSKTTL
                     ATLKTARTNRTRIDWEGYAPPKPNFLGVRTFDDYALQEIRPYIDWTPFFHSWQLKASH
                     PRIFDDPEKGPEARKLFADAQDLLDRIIAGKWLSASAVFGLFPANALEDDSIELYTDE
                     SREETLTSLHFLRQQRQKPPGRPNRSLADFVAPRSTGLEDHVGLFAVTAGLGADEVAL
                     RFERDNDDFNAIMVKALADRLAEAFAELMHRRVRTEFWGYASDEDLDNEALIREEYNG
                     IRPAPGYPACPEHTEKRTLWSLLDVENNAGVTLTENCAMTPGATVSGYYFSHPDCTYF
                     GVAEIGRDQVEDYAERKGWTLEEAEKWLSPNLGYDLEAGQKSNGASSVQQSVIRSAR"
     gene            complement(37841..38470)
                     /locus_tag="VN178_06445"
     CDS             complement(37841..38470)
                     /locus_tag="VN178_06445"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HWS80648.1"
                     /translation="MGRKGGGLGTTLVLALVIVVLGVALGVGLARFGSSLPIVGPLLG
                     EKPPRTTTGPVVVEGIEELDHLATVRWTESVPVTRETGGDILERLFSGEKVIVIATGK
                     VEAGVDLGDIEMDDVAVDGDSVTIDLPEPEILSVSLDEGRTRVYDRDFSPLNVRPDDN
                     LVEMARLRAVQKIEDAARENKILETAERSAEDSIRAFAATLGFDEVRFR"
     gene            complement(38560..38772)
                     /locus_tag="VN178_06450"
     CDS             complement(38560..38772)
                     /locus_tag="VN178_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HWS80649.1"
                     /translation="MAGLEDILARYRPYGPTYTPGEKAPFSGTYICDRGTMLPPVRVQ
                     LSRGEEFPEASGLGEHTRWRQTNIQA"
     gene            complement(38772..40340)
                     /gene="serA"
                     /locus_tag="VN178_06455"
     CDS             complement(38772..40340)
                     /gene="serA"
                     /locus_tag="VN178_06455"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWS80650.1"
                     /translation="MRVLVSEKLADPGIRLLEQDFEVDVLLDLGPEELLQKIGDYDGL
                     IVRSATQVTADVIERAGNLKAIGRAGIGVDNIDIESATKHGIIVANAPESNTVAAAEH
                     TLGLMLAAARRIPAADSSLRGGEWKRSALKGVEVSGKTIGLVGLGHVGAIVARGALGM
                     GMQVLAYDPYVSEDRMRSMNVERAQTADEVFERADFVSLHVPRTPQTMDLVNEDALAK
                     MKSSAYLINVARGGIVDETALYNALKEGEIAGAALDVFAEEPTTDSPIFTLPNVVVTP
                     HLGASTAEAQDRAGITAAEQVATALRGAVPVHAINAPVPMGEGAEFVAQFSGLCESLG
                     SLLYQLTDHAGDILRIEYHGEVAGFDTRLLDVSAQKGLLAPMVFEPLNFVNTPALAKE
                     RGLKVETSRSSESTDYTSLVELRMGEMAVSGTLFGPRMQPRLVEAMGFDFDMVPEKYM
                     LFIQNEDRPGMIGKLGSILGDHNINIGNMAVGRGEPGSRAAMAVTVDEPIPEEVLNAL
                     VETPGFDDARSVTL"
     gene            complement(40337..41494)
                     /locus_tag="VN178_06460"
     CDS             complement(40337..41494)
                     /locus_tag="VN178_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWS80651.1"
                     /translation="MMNKYRLMSPGPTPIPPEVSAAGALPIIHHRTPEFGEVFTRVNE
                     NLKRVFLTENDIFTYAASGTGAFEGAIQNLFSPGDKVLVVNNGNFGGRWVKMGRAFGL
                     EVTELQYEWGQKADNDEVAEALAGDPGIKAAICVLSETSTGTVNDIEGFARAAQNVVT
                     IVDAVSGLGACTLRTDEWGIDVVVAGSQKALMTPPGLGFVSVSPRAMRVHEESRMPRY
                     YFDWTAARKAYQKDPAQTAWTPAVSLIIQLDVALRQLLEEGVENVFERHVLLGRASRA
                     GIKGMGLELFGPDEDPNSAVTAAWVPEGTDGKQLVRLVFREHGIQVAGGQGEMAGRIF
                     RIGHCGFFDAFDIIATIAATELALESLDYPVELGKGVGAAQRVFSKSGVKA"
     gene            complement(41773..42954)
                     /locus_tag="VN178_06465"
     CDS             complement(41773..42954)
                     /locus_tag="VN178_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS80652.1"
                     /translation="MPDPAGLTRLAVIGLGHRATAMIASMQAVDPGVSVRAVADPDTE
                     GAGRRLAMVGNAGVRLYPSADAMLEDPADYDGVVIGTRCHLHTRMAIKVASVGLPLFL
                     EKPVAISPEELGALREAFRGREDEVAVSFPLRHTPLFSKVLEVVRSGALGTINQIQAV
                     NNVPYGGVYFGQWYRNHDQTGGLWLQKATHDFDCINLLAGSRPALIAAMESRRVYGGE
                     KPHDLRCSACDETETCPESPQNLALRGDDGGMYQDSDDRTDHWCAFSREIQNHDAGSA
                     LIMYENGIHASYSQNFLSRRSAYKRGATVIGYEATLEFDLALMELRIIHHRSEKVEQM
                     RVDVEDDGHAGGDLILAEGFLDLVKRRASPNASLRDGLLSAAMCLAARESARTKTFQW
                     V"
     gene            complement(43005..>43269)
                     /locus_tag="VN178_06470"
     CDS             complement(43005..>43269)
                     /locus_tag="VN178_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80653.1"
                     /translation="LEELIIQTASGLDVEDQKATVTKLSKIFNELLPVIPLWERYGNN
                     PALDGERVTGWLPDSNPIYVNSPYEDSFTVMMIMDGTLKPAGQ"
BASE COUNT         7883 a        13939 c        13533 g         7914 t
ORIGIN      
        1 ttctagcctg tcctcgacga cgatcttgcc cggcgccgcc gtgcgggggt agaacgagct
       61 cgggaagtgc tccgtgtgga agttgggcgc gtcgatcgcc tcttgcaggt tgaggccgaa
      121 gtgcacgtgc gccaggaaga agttcaggga ccactgatcc tgctggtccc cgcccggggt
      181 gccgaaggcc aggtacggct caccaccacg tagcgccagc gagggggtga gtgtggtgcg
      241 cggccgcttc ccgccggcca tggagttggg cagaccttcc gtgagccaga acatctgggc
      301 gcgggtaccg agagggaaac cgaggccggg gatcacgggc gaactctgca gccatccgcc
      361 gctcggggtg gccgagacca tgttcccgtg acggtccacc acgtctacgt ggcaggtgtc
      421 cccgctgccc tggtcgtcgc gcggcaccgt aggctcgcct gctccggtgg actggtccgt
      481 agccgcggac ggcgggtcgg gaagtcgcgg agaacgcccg ctggccgcgc cgggacgtag
      541 ttcgaaagag gcatcttctc cgatgaggtc cctccgcgaa tccgcgtatg agggatcgag
      601 caactcctca ggaggtacgt ctacgaacct tgggtcaccg tagtgggcct ccctgtcggc
      661 gaacgcgagc ttggcgcact ctacgacggt gtggatgtat tcgacggtgt tgtggcccat
      721 gccgacgagg tcgaacccgt cgagcaaagc gagctgctgg agaaatacgg gcccctggcc
      781 ccacagcccc gtcttgcaca ccgtgtagcc gtggtagtcc agcgtcgccg gcccttctag
      841 ttcggccctg taagcggcga agtcgtcgcc gcggagcagg cctccgtggc gccgtcctga
      901 ggtgtccatg acccgcgtcg aggtcacgta gcgcacgatg gcctcggcga cgaacccacg
      961 gtagaacgca tcccgggcgg cctctatctg ctcgtcgcga tccgcgcttc tcgcctcggc
     1021 ctcctcgacg atgcgcctga gggtacttgc caggtcgggg ttggagaaca gggtgcccgg
     1081 tttcgggatt tcccctccgc gcagatagac ccgtgccgaa gaggtccatt cgtcacggaa
     1141 cagcttctcg accctaccta tcgtgtcgct gatcttgggc acgagcgggt agccgtgccc
     1201 ggcatagtcg atcgcgtagc gcatcacctc agagacgcga agcgtaccga actcgccgag
     1261 caggcgcatc cacgcgtcga acgcgcctgg cacgcaggcg gcgagcaacc ccgtaccggg
     1321 caccaactcg aggccgagct cctcgaagtg cgcgatgctc gccgctgccg gcgccggacc
     1381 ctggccgcct atgaccagcg tctgggccct ttcggcgctg tggaagatga tcgggacctc
     1441 cccgccagga ccgtttaggt ggggctcgac gacctggagg gtcagcccgg cggccacggc
     1501 cgcgtcgaag gcgttacccc cggactccaa tacggccatg ccggccgccg aggccagcca
     1561 gtgcgtggag gcgaccatgc cgtgggtacc gacgagttcc ggcctggtgg tgaacatgca
     1621 gcgaccctaa gggatgcagt ggcacgcggc gacccagtgc tccgggctga cctcgcgcca
     1681 tacgggctca tcgtggccgc acgcttcggt ggcgtagggg caacgggtcc tgaaacggca
     1741 acccgaaggg gggttcactg gagaaggcac gtcgccttgc aggactatgc gttcgcgctc
     1801 gagctcgacg tcggggtcgg ggacggggac cgcggagagc agggcttggg tatacgggtg
     1861 caggggattg ctgtagagct catcgctggg cgccatctcc accatcacgc cgaggtacat
     1921 aacccccacg cggtcggaga tgtatttgac caccgagaga tcgtgggaga tgaacaggta
     1981 ggtcaggccc ctctccctct gtaacttctt cagcaggttg atgacctgcg cctggatcga
     2041 aacgtccaaa gccgagacgg gctcgtcggc cacgatgaag tcgggatcga ccgcgagcgc
     2101 ccttgccacc ccgatcctct gccgctggcc gccggagaac tcgtgtgggt agcggttggc
     2161 gtgctcgggg ttcaggccaa ccgtctcaag gagctcgtgc acccggtcca ggcgttcttt
     2221 gccgttcgcg gccaggttgt ggatgtctat gccctcggcg atgatgtcgc tgactatcat
     2281 gcgcgggttc agtgaggcct gggggtcctg aaagaccatc tgcatcttgc ggttgagggc
     2341 cctgccctcg ctgcccgtcg cctcgtggat gctcttgccc tcgaagagca cctcgccgcc
     2401 agaaggttcg tagagcctga ccagaaccct tcccgccgtg gacttgccgc agcccgactc
     2461 ccccacaagg ccgaacgtct cgcccttgtt gatctccagc gagattccgt cgaccgcctt
     2521 caagttctcc cggcctaccc ggaagtactt cttgaggtct cttatctcga gtagcggccg
     2581 tccgttcgct tcgccgttct gggtctgttc cgtcatttcg cacccccgat gctcgcggga
     2641 ggctcgactt tgggggccat ccgatggtgc agccagcaag ccgccctgtg ctcgtcggag
     2701 aacgtagtgt agcctggcat ctcctcctcg cagatccgca tggcgtaggg gcagcgggca
     2761 ctaaagggac agcccgcggg cgggtcgagc gcgttcggcg gcgagcccgg gatcgggatc
     2821 agctcctggc tccggtccgc cgtcggaagc gggatggagg ccagaaggcc ccaggtgtaa
     2881 ggcatctggg ggttgtagaa gatctcgcgc accgtgcccg tctccacgat cttgccgccg
     2941 tacatcacac cgatgcggtg ggcggtgctg gcgaccacgc cgaggtcgtg cgtgatcagg
     3001 atgatggaca tgccgagcct ctcctgaaga tcgcgcatca gctccaggat ctgggcctgg
     3061 atcgtcacgt cgagggccgt ggtcggctcg tcggctatga ggatctgcgg atcgcaggag
     3121 agggctatgg ctatcaccac cctctgacgc ataccgccag agaactggtg cgggtactgc
     3181 ctgaggcgct cctcggcgtt ggggatgccg accagctcga gcagctccac ggccctctcc
     3241 gccgcctggg cgcgcgacat cccgaggtgt ttgcggaggc tctcggagat ctggcgccct
     3301 atcctcatgg tggggtcgag cgaggtcatc gggtcctgga agaccatcgc gatcttagga
     3361 cccctgatct cgagcatgcg cctctcggag agcctgagga gatcctcgcc ctcgaagacg
     3421 atctcgcctc ccctcacgat ggtgttcgcg gcggggttga ggcgcatgat gctcctcgcc
     3481 gccaccgact tgcccgagcc cgactccccg actatcgcca gggtctcgcc gcgcctgaga
     3541 tcgaaggacg ccccgcgtac tgcttgcacc tcgccggcgt aggtactgaa ggagatccgg
     3601 aggtcgtcga cccgtagtac cgcgtcgctc gttccgttcg tgctttcgtt caccatccta
     3661 cttccgcatc ttggggtcga gggcatcgcg tagtccgtcg gcgagcaggt tgaagctgag
     3721 catgagcaga cagaacaccg ccgccggaaa gaacaggagg taggcataga atcgcatgtc
     3781 ttcggcgcca gcgtcgatga gggagccgag cgaggcgttc ggcacctgga tacccaaccc
     3841 gataaagctg aggaccgctt cggtgaagat ggcgctcggg atggtgagca tcagcgtgat
     3901 tattatcagc cccagcgcgt tcggtagcag atgctttgtc aggagccgcc tgtttccggc
     3961 ccccagcgag cgcgaggcga gggtgaattc ctgatctttg agttgcagga cccggcctcg
     4021 gatcaggcgc gccatcggcg tccagcccgc cgcgcctatg gctatgctga tcgagagaat
     4081 accggggtcc aggaccacca tcgccaggat ggctaccacc aggctcggta tgccgttgag
     4141 gatctcgatg aaacgctgca tgacgccgtc caccttgccg ccgagcaacc cggagactgc
     4201 gccgaaggtg acgcccacga ccaggtcgag accggcggct atgaacgcga tgtacaaaga
     4261 gaccctcatc ccctgccaca cccgggcgaa catgtcccgt ccgaactcgt ccgtgccgaa
     4321 ccagtgctcc gcgccgggtc gctcgtaacg ctgggcaatg tcctgctcgg agggcgagaa
     4381 aggcgttagc aaaggtccga ggacgaaggc gaaagcgaac aggattacga tcaatgccag
     4441 gctcaccagc gccgcccggt tcttcttcag gcgctcccac gagtccctcc agaaccctat
     4501 cgaggggccg gcgatcctct cgctgtcccg cgagccttcc tcgagcgccc ggaattgctc
     4561 cggcgtaagc tcgcgatcgc gagtctccat actctactcc tctcccccgg cgacgcgtat
     4621 ccgagggtca atcaccgcgt acaagatgtc ctggatcagg tatgccagaa tgaaaaggac
     4681 cgagaagaag atcgtcgtgc cgagtatcgt cgcgtagtcg ttgacgaata tggagcgcac
     4741 gaacaggtcc ccgatgccgg gaaccgcgaa gatctgctcg atgactatgg agcccgtgat
     4801 gatccccacc atgagcggga acagcaccgt gacgagcggt atgagcgagt tacgcaacac
     4861 gtgcctccag accaccgcct gccagctgag gcccttggac ttggcgagcg tgatgtagtc
     4921 ctgccccaga acctcgagca tctctgcccg cacgtaacgg gccactatcg cggtcacgaa
     4981 caccgcgagc gagatcgaag gcatgatcga catggtccat gccggggcga aggggtccca
     5041 cgctatgggc aatagctccc accggaagcc gagccagtac tgcagtatcg gcccgagcac
     5101 gaagctcggc acggcgatgc ccgccacggc caccgtcgtg cccaggtaat ccagcacgcc
     5161 gttgtgcctg agggccgcca agacacccag cgcgagaccc ggtatcaggc cgaatatgat
     5221 cgcctgcacg ccgataaaag ccgagaccgg agcttgctcg aggatgatct gggtgacggg
     5281 acggctctcg aagtagaagg agttgcccag gtcgccctgc gccacgttga gcatatacct
     5341 cacgtactgg acgggcagag ggtcgtccag accgtacttc tcgaagagct gcgcccgaga
     5401 gctgggcgtg agcttctcgt cggcgaacgg cgtgcccggc aacgccttgc tcaacaccca
     5461 cgtcaagctg atgatgaggt agagcgttat gaacattatg atcagacgcc gccccacgaa
     5521 cctcagcatc ccgataccgc ctttctagct ccaaccttca cccggcccgg agaccggacg
     5581 cgggaggccg ccctcggtag ggatacggcc tccacatggt atttcgcaga agtccacgct
     5641 acgggctacg ttagctctgc aatctccaca gctttatcgc gtcgccgccg gagcggtagt
     5701 agacgaagtt cttgatggag ggttctatga gccaaacctg gccctcgaag taaaccggcg
     5761 cgaggcctgc gtcttcctcg atcaggaccc tctcggcctc cagcatctgg ttcatacgcc
     5821 tggcgaagtc cgtctccgtc agtgcctggt tgatgagctc gtcgtagcgc tcgctcttgt
     5881 attttccggt gttgaaggag gagtcggaga ggaagaggtc caggaaggtc atcgggtcgt
     5941 tgtaatccgc gatccagccc tggaacgaga gctggaactc accgtccgcc tccagctcca
     6001 gcttccggtc gaagggctgg accgtgacgt tgatcttggc ccccatcttc tcgaactgct
     6061 cttgcaggaa cgtggcgacg tccctccccc cggtggtgtc ataggagaga agctcgatgg
     6121 tggggttctc cccgatctct tcgacaccct gttgaaagag tctcttggcc tcctggagat
     6181 cgaactccgg catggtcggc ccctcggcct ctcggaaggt ctggctaccc ggtccgtcta
     6241 tcccgttcgg tacgagcccg acccccggct cggaaccatc ctggaggatc tcctgcgtca
     6301 gggtctgctg gtcgaagccc atctggaagg cacggcggat gttctcgttc tggaagagcg
     6361 gcagatcgtt gttgaagacc aggtagaacg tggcgaagtt cgactgcgag tagaagtccg
     6421 gggagtcccg gtactggttc acgtactcct ggctgatctc cgtgacgtct agattcccgg
     6481 cctcgtgaag gttgacggcg gtcccgatct ccgtgacgat ccttccttcg atcctctgaa
     6541 tgtcgacgtt gcccgcgtcc cagtagtcgt ctctcttgac gtaggtgacg ccctgggtcg
     6601 ggttgaagcc ggtgagacta tagggaccgt tgtagaggag ggcatccgcg ctctgaccat
     6661 actggtcgcc ctgttcttcc acgaagctct gcttctgcgg caagtatgtt gtgaacgccg
     6721 tcaggcccag gaagaacggc gacggactca ccagcgtcac ctcaagcgtc ttgtcgtcgg
     6781 gcgcctcgat ggcgacacca tcggcgctgc catcgcccga gttgaactcc tcggcaccct
     6841 ctatgaacgt gtagagaatg tatgcgtact ggcccgccgt gtcggggtcg agccccctga
     6901 gccaggcgta ctcgaagtcc ttcgaggtca cggggtcccc gttggaccac tttacgccgt
     6961 ccctgagcgt gaacgtgtaa gtgagcttgt cgtcgctgat ctggacaccc tccgccatcg
     7021 ccggctgggg atccagattc tcgtcgagcc ggtagaggcc ttccataacg ttgatgagta
     7081 tgcccgagga gaccgagtcg gtggtggtcg tggagtccag gtccctgatg gtggcctgca
     7141 ggttcaaggt gatggagctg ccgccgcccc caccaccacc ggttccgcct ccgccgcctc
     7201 cacccccgct gaagatgcca cacccggcgg tccctaggag ggcagccccg gccaggccgc
     7261 tcccgctgat cttcaggaag tccctgcggc tgaactgtgt gccgaccagt gccttacccg
     7321 gcctgactct ccttatggcc atctgatcct cctttcacct ccagcgcccg atgcgccaga
     7381 gtccaaacct tctactacct ctatgtttta cggacagacg ccgaatgcag acgccccacc
     7441 ggcgcctacg ggtcacacaa cccccagacg cccgtcccca taaggatact cctgtacggg
     7501 gcccggtgcc tacacaaacg gtttacaact ttccatacta gtctacttta agccaactgt
     7561 aaccgggata agtaaaggat gtcaagcctc gccccggcgc tcacaggaaa ccttaacgaa
     7621 acgtagggag cgagaatcta catcctcatc ctcgggtcga gcgcgtctcg gagcccgtcg
     7681 ccgaggagcc tgaagctaac ggtgatcaag gcaagcacga tcgccgggaa gatcagcagg
     7741 tacggataga acctcatctg gtcggccccg tcgctgatca gagccccgag cgaggcgtta
     7801 ggcggctgaa tgcccaaccc gatgaagctc agaaacgcct cgaagaagat ggccgagggc
     7861 accgtgaaca taagcgtgat gatcacgatg tagaagacgt tggggatgag gtgcttcact
     7921 atgaggcgga aggtacctgc ccctagagac cgcgaggcca ggaagaactc ctgctccttg
     7981 agcctcaaga cctggccccg gatcaggcgg gccgaactcg gccagctggt tatggccagg
     8041 gcgagggtga tcgcgattat gccaggctcg aggatcagca tcatcaggat gatgaccacg
     8101 aggttgggga tgccgaccag gacctctatg atacgttgca tgacgttatc caccccgcca
     8161 ccgtagtacc cagagatggc cccataagac atcccgatgc ttatgtccag ggcggttgcg
     8221 aggagtgcaa tgtaaagcga gaccctggtg cccacccaca cgcggtcgaa catgctgcgc
     8281 ccgaactcgt cggtgccgaa ccagaaaccg gagagcgacg gctctcggta ctgtcgttcg
     8341 agatcctggg cgaagggggt gtaaggtgtg agccagggac cgatgacggc caggatgaag
     8401 accagaaaga ttatgccgcc cgcgaggagc gcgatgcggt cccgcttcag tcgccgccag
     8461 gcgtcgctcc agaagccagc cgggggggcc gagagctcct cggcgtcccg gacgctcctc
     8521 ttcacgggct cgaagagatc ctgcgtcagc gtgatctgtc tgtcgcctct ctccatatct
     8581 ccccctaatc cccgtgcccg accacgcgga tacggggatc tataatcccg tagagaatgt
     8641 cttgtatcag gtacgaggcc acgaagaaga ccgagaaaag catggtggta cctatgatca
     8701 tggagtagtc gtttacctgg atcgagcgca caaactgctc gccgatgccg gggatggcga
     8761 agaccgtctc gacgatcagg gtccccgtga ccagggcagc ggtgagggga acgatgatgg
     8821 ttatgagcgg aatcatggag ttcctgagca cgtgcctggc gacgaccgcg accttgttca
     8881 ggcccttgga cttggccagc gtgacgtagt cctgccccag aacctcgagc atctcggtcc
     8941 tggtgaaggt cgccacgacg gcggcgacgg tgatggcgag caccatcgag ggcaaaacgg
     9001 agtgccacca gctctcgaag aaggctgggg ggaagatccc cagcttgaaa gcaagccagt
     9061 actgcgccag tggcgcgagc acgaactcgg ggacggcgat acctagcacg gcaagtaacg
     9121 cagtcccgct gtccgcaagg ctgttgtgct tgagggcgcc gatgacgccg agcaacaggc
     9181 ctcctataac tcccagcacc actgcctgag cacccagaaa agcggagacc ggcagccgct
     9241 ccaggatgat ctccaggacg ggcttcccgc tgtactggta ggagttcccg aggtctccct
     9301 gcgccagatt acctatgtac ctgacgtact gcaccagcaa gggatcgtcc agaccgtact
     9361 tggcctcgat ctgcgcccgc gcctcgggcg agagcttctg gtcgttgaac ggcgagccgg
     9421 gcaggagctg cataaggaac caggtcgccg tggcgatgac gaatagaatg acgcaggcgt
     9481 tgaccaaacg cttgagcgtg tatccaatca ttacctatct ccccgccgag atctcaccta
     9541 cttcccttct atgctcgcat acttgaagtc ggggctgccg ccgaaatcgt gcggcgcgaa
     9601 gcccttgaag tacggcttct tgagaccggc gaccgcggaa tagtactcgg ggatgagcac
     9661 gacctcatcg aacaggatcg gctcggcctc cgccatcatc tgcatgcgcc gctcctggtc
     9721 gccctctcgc ttcgcgccat tgatgagctc gtcgtacctc tcgctggacc acccggtagt
     9781 gttctgtggc gaatcggaga ggaagaggtc caggaaggtc atggggtcgt tgtagtcggc
     9841 cccccagccg gagacgaacg agacctggta gtctctctcc tgcaccctgg tcagcccggc
     9901 ctcgaaggtc gtgatctcta tctcgagatc ggcacccagg ttctccttgt actggccttg
     9961 tataaatgta gcgatgtcgc ggccgatgct gctgtcgtcg aagagcatct tcaaccgcgg
    10021 cgtctcgccg agctcctcga cgccctgctc ccagagctgc ctggccccgc tcgtgtccgt
    10081 cggcaggagg tcgcccaggg cctgcctgaa ggtctggtcc ccgggcccgg gcgtgatggc
    10141 cggcgggacc agagcgtagg ccggctcgga gccgtcggcc agtacccgct cggtgagggt
    10201 cttcctgtcg aagccggtca tgagcgcctt gcggatgttg aggttcgcca gcgccggcac
    10261 gtcgtggttc acctggccgt agaaggtcgt gggttccacc ctccggtaga actcggggct
    10321 gtcctcgtac cttgcgacct tgtctcccac caggccggtt atgtccagct ccccagcctc
    10381 atagaggttg agcgcggtgt cggagtcctt gacgatccgc ccgttgatcc gctggacggc
    10441 gacgttggcc ttgtcccagt agcggtcgtt cttctccagg acggtcgtcc ccccggcgcc
    10501 gacgccaccc tgagtcatga cgtagggacc gttgtagatc agggtgtctg cgtcctgggc
    10561 gtagtcgtcg ccctgttgct cgacgtactc ctgctgctgg gggaagtaga gcgggaagga
    10621 tgtgagctgc aggaagtacg gagccttgtt cacgagtgtg acgcgcagcg tcctgtcgtc
    10681 cggcgtctct atggcgacgt ccccggcgct gccatcgccc gcgttgtact ccgcggcgcc
    10741 ctgtacgaac gtatagataa tgtaagagta ctcgctcgcc gtgtcggggt tgagcacctt
    10801 gagccaggcg tacttgtagt ctcgagaagt cacaggatca ccgttggacc actctatgcc
    10861 gtcgcgcagc gtaaaggtgt aggtgagccc gtcgtcgctg acctccgcgc catcggcggc
    10921 ggccggtacg ggcttctggt ccgggtcgag cctgtagagg ccttcctgcg tgttgaccag
    10981 gatcccgaac gagatctcat ccgtggcaag cgcggagtcc aggtaacgaa tctcggtcgc
    11041 gaggttgacg ttgaggacgt tgccgccgcc cccgccacca ccggttccgc cgctattctc
    11101 gaagacgccg catccggcgg tccctaggag ggcagccccg gccaggccgc tcccgctgat
    11161 cttcaggaag tccctgcggc tcagctgtgt gctgaccagt gccttacccg gactgaccct
    11221 ccttatggcc atctgatcct cctttcacct ccagcgcccg atgcgccgga gtccaaaccc
    11281 tctacacaac ccccaggtgc tcgtccccgt aaggatactt ctgtacgagg cccggtgcct
    11341 acacaaaccg tttacagctt tccatactag tctactttaa gccagctgtg accgggataa
    11401 gtaaaggatg tcaagcctcg ccctggcgct agcctgagcg tgctgacaag cgcccgacag
    11461 ggcgcgtgct gaaatgcttg ctatcctccg gcgcggtcgc ggacgtaggc ctcgtaggac
    11521 gggtgtgagg cgcggggacg gcggcgagag aagagggcga ggatgatggc accggtaccg
    11581 agtagagaga gggcgtagag gagcaggttg ccgagcacag gaatgaaggt ggtcgcggcc
    11641 aggatcatag cccccaccac tgctgccagg gcgttcccga ccctgtagcg tccggtgatc
    11701 ataaggaccc tggtgccgat gaagaaagcg gccaccagtg ccccgaagaa caggagcgcc
    11761 agataagccg gtacgagaag cagcaggagc ggtatcccta caatgctgac cgcgagcacc
    11821 acgcagagaa cgaacactac cggcaaagag agaagcccgt acacgaagga cctccccgga
    11881 gactcctcgg ccctgccggc cgccgaggcc ataggccgtg gtgccaccac ggcggcgagc
    11941 acggcacagg ccgcgaacgc gagggccatg aagagccagc caaagaagcc tactaccccc
    12001 ggcccgtggg actcgtcgaa gtcgagcgcc atccccctcg cggtcatgtg gccgtccacg
    12061 gcgctcctgt tgccggtgat ctcacatccc tcgcactcca ggtcacccga gatctcggcg
    12121 tcagggccaa gctcgacgtc gccccatcct acgttcaagt caccttcgac cgaatcattg
    12181 acgtaaacgt ccccgaactc ggcgcgaacg tcgccttcca cggggccttc taacttcacg
    12241 ttcccgaaac ccgcgtcgat gtcaccctct actctgccgg ttacgaggat gtcgccgaac
    12301 ccggagtgca cgtcgtcccg tgccagtccg cgtacctcga ggtcgccgaa cccggtggaa
    12361 acgtcgtgct cgacgcctcc atggccaacc accacatcgc caacgagccg ctcttcagcc
    12421 gcgtgcgcgg ggaaaggcag gagcacgacg agaccgagga tcacgaccga aaaggtaagc
    12481 aggggtaggg cgtcgaccct gatggaagag ggtagagagg ggttcatgcc tctctggcgc
    12541 gccggcggta gaccgccagg tatgccgcgg ctataacgag gtccacgaaa gcgccgacgg
    12601 cgagcgcgat caggagcgcc ttaccgatgg cggcgaagac gacctggatc acgtcggcga
    12661 ggccggccac gaatccccag aagacgttca gagcgtcgac gacgaagacc gcgaggttcg
    12721 ttccgaaaag gatcatggca accgaggtcg taagcagcag aacgacggcc agcacggacc
    12781 agaggaacct tgcctttgaa gaccgggtcg gcagcgccat cgcaacgctc cggcacaccg
    12841 agttggtctc ctcgaactcc agggagccga gggaagcgtt gagcagcagc tccctctcgt
    12901 aaagtgacag gcagccgcca caatctctca aatgcgcccg gatctcacgc tcccgctccg
    12961 gtcccaacga gccatcggcc agctcgaaga cggactcggg atcgcaggga agcggccttc
    13021 tcactcccgc tcacccgcct cttccagctt tccggcgatc atgttgcggg cgcggaacag
    13081 cctgctcttc accgcgggga ccgagatgga gagcacctcg gctatctccg catagctcag
    13141 atcctcggtg taacgcagga tcagcggcac gccgtacgcc tcgggcaggg tcgcgatcgc
    13201 ctccttaacg ctcctggcac ggtcggcctc gacggcgtcg gattcaggac tctccgagac
    13261 aggggcgtgg gcttcggggt tgaactcgac gatctccctg cggcgcctaa gggtgtccac
    13321 accaaggttg ttggcgatcg cgagcagcca cgtcttgaag cggtagtcgg ggttgaaagt
    13381 ctccaggcgg tcgtaggcgc gcaggaacgt ctcctgaacg aggtcttcga cggagatctc
    13441 acgccgcgcg tagggatata cgacgcgtcc gaccagccgc tcgtaacggc ctaccagctc
    13501 ctcgtaggaa cgcaggtccc ccgaaagcgt gcgtgcgacg agctccttgt cgtccagctc
    13561 ctcgcgccgg accgacaggc tccccagctt ctcccgccag ccacctagtg tcaccgtgga
    13621 tgtatactcc atactaagac gagtatacgc tttcggccca ggaaagtttc cggaagtcga
    13681 gaggatcccg gcgagaagtg gaactgccta aagcgatctg cagaggcacc ctctgcagat
    13741 cgcgcctcga gcatttcagc gagtcagcaa gaacaagctg aaacgctcga tcagatcgcg
    13801 gcctgcccgt cgatgctcag gccggataac ctggaggcgt gctcggcgag ggcctccccg
    13861 ttgactatga tgatcctgcg gttgcggctc tctattatcc cgtcgcgctg gagctcgctc
    13921 atcaccttgg agacggcctc cctggtggag gcgatcatgt tggcgaggtc ctggtgagtg
    13981 aggcgcacgt ctatctgcgt gccagcaccg tccctctccc caaatcgttc gccgaggttt
    14041 atgagcagag tcgccagcct ggtggagacc tccctgtgca gcaggctctc tataacctca
    14101 tcggactgcc ttagccgctc ggagaacgaa gaaaagagct tgagggcgaa gccgggatcg
    14161 ctctttatca cctgctcgat ggatgccttc tgtatgctcg ccacccgtgc ctcggtaacg
    14221 gcctcggcga agacgtcctg ccagcgtccc tcgaccaggt tgagcttgcc gaagacgccg
    14281 ccttccttga ggagcgcggt cgtggcctcc ttgtagtcgc cgtagatctt gtacagccgc
    14341 accgtgccag agagcaggaa atagagttga tcgtcgggat cccccggcgt aaagatcgtg
    14401 tccctcggac cgaaacgccg ctctacagtc ctgagactgg cctcttcgaa gtcctggaga
    14461 tcgaaccctt ccggtgcgct tgtggtagcg aaacctccgg tgctgtggct cgtgagcatc
    14521 ttaagcgacc tctctaatga aacgctctgt aaacccggcg aaagtcccgg actcgcatat
    14581 tatacccgat gttctaattt ctattctcga tcggcgaggg gtcttctagc gccgtatggc
    14641 caggatccct caggccgcac tccggcgcca cctgagctcc acacccgcac gcttggcgac
    14701 gaagagaagg tcgtcgcgca gggccccgat gtccttgtcg tagatggtga ctatacagaa
    14761 atcgcagtta cggtacgccc aggcgaaggg cgcgagatcc ccggccaccc tcctctcgga
    14821 gttcatgccc caggcgtcgg ccagcctcag gcgtccctcc gcaagctcca gggtgatccg
    14881 ccatccctcg tccgtcggga cctcgaagta gcccggcaga ctaccggcgg ggatcatggc
    14941 gttctgcagg tccttcgcaa acgccctctt gtgggcctcg accccggcga ggtagtccgt
    15001 gtcgtccacc gacctcgggg gggccggtgt gtagagtcga ttctgcgtaa cccttactcc
    15061 ctcaggcggg tagaagccga taaaagggac cggcgagcgc cagagatgct gtatcacccc
    15121 gagcgtcacg ttgcggcgct ccgcaggctc tccgacggac tcggcgaaac gcatcgccgt
    15181 caggcgcatg tgtcttggct cccacggatg gggctctccc acggtccgta gccgcctccg
    15241 gcgcttggtt atacgactga tctcttccat gacgcgcgta ttctacaact ctcgtgcggc
    15301 ccatgcccga gcaaggaaaa gttcgagggc ggggccgaag ccccgccctc atggtcgaga
    15361 ggaaaggtcc ctgcgctact gcctgctcac ggccagcagg tagaggaagg tcagcaccgc
    15421 cgtaagagca gcagcgatgt acgtgaatgc cgccgcggtc agaactcgcc tggtacctcc
    15481 tgcctctgta cccgacagta tcccgtagcg tgtcaggagc ccgtaggcgc gggtggaagc
    15541 gttgatctcg accggcagcg tcaccacgtg gaacagtaga accccgagga acaacacgat
    15601 ggcgacgttg atcaggagcg acccgaacgg gctcgccgca cctccgacga agaaagccat
    15661 gatgaaagcc agaggccaga tctggctgct gaagttcacg atcgggaaga tcccggccct
    15721 cagcttcatc ggcacgtagc cgctagcgtc ctggatcgcg tgccccacct cgtgggcggc
    15781 cacgcttacc ccggctatgg aattgcccct gaagttgtcc tcggagagcc tgatgacctt
    15841 gctcctcggg tcgtaatggt cggtgagatg cccggccaca ggctctaccc tgacgttcgt
    15901 aaggccgttg cggtcgagta tcatgcgcgc cgtctgggcg ccggtgaggc cgctcgcgct
    15961 gcgctgcttc gagtacttcg agtaagagct cctaacccac agggccgcgg ccccagagat
    16021 gagcatgccg acgatcatta ttagcaggta accgccgaat cctcctggta tgaacaacgt
    16081 caatcctcct agcagattta ctgcctgtat tttactacat acatatacgc agggtcgctc
    16141 gcaaagttcc gcgaaacgaa ggcctcaggc atcgggctac aggtttcagg attaccatag
    16201 aatagtctct ttggcgaaaa gagttacgct gccgcgtcat aggctcggat cttgtaaaga
    16261 gcatcgcgag gtaccggagc ggtcacccgg gtggacaagg gtcggggcgg ggtcttcgag
    16321 agtcccgccc cgtattcgag gccccttgtc gtctggctgc tagctctggc tgcgggcgtc
    16381 cttgagatcg tcgacgacgg aggggtcggc gagggtcgat gtgtcgccga ggtcgtcctc
    16441 gccctcggcc acgccgcgaa ggacgcggcg cattatcttg ccgctcctcg tcttcggcag
    16501 gtcgggggtg aagattactt cctccggcct ggcgtgcggg ccaatggcct tcctgacctg
    16561 gtcgtggagc tccttgatga gctcttcggt cccctctctg tcgccttcca ggatcacgta
    16621 ggccacgata gcctggccct tgtcttcgtc gaaccgcccg ataacggcgg actcggctac
    16681 gtgcgtgtga gagacgaggg cgctctcgac ctccatcgtg ccgatgcggt ggccggagac
    16741 gttcatcacg tcgtctatcc tgcccgtgat ggtgtagtaa ccgtcttcat cccgctcggc
    16801 gccatcctcg acgaagtact tgtcgccgag tctctcccag tatgtctcgc ggaaacgctc
    16861 ggggtccttg tagatcgtgc ggagcatgcc gggccacggc ctggtgacga ccaggttccc
    16921 ctttccggcg ccttcgattg ggttgtcgtc ctcgtcgtag atgtcgacga agatgccggg
    16981 gaatggcctg gtggcgctgc ccggcttggt cggggtgagg ccggggagag gcgcgatcat
    17041 gatgccgccg gtctccgtct gccaccacgt atccacgacg gggcaccttc cgccgccgat
    17101 gttctcgtgg taccactccc acgcccgcgg gttgatgggc tcgcctacgg taccgagcag
    17161 cctcaacgat gagagatcgt gtttctcgat cggctcggta ccctgcttca tgaacgcccg
    17221 gatgacggta ggcgctgagt agtagatcgt aacgccgttg cgctcgatca cgtcccagtg
    17281 gcggtccggg cccgggtagc tcggcgtacc ctcgaactgt acggtagtcg ccccgttgct
    17341 caagggtccg tagacgaggt aggagtgccc agtgacccag ccgatgtcgg cctggcacca
    17401 gtagacgtcg tcctctttga tgtcgaggac ccacctggtc gttgccttga cctgcgtgag
    17461 gtagccgccc gtggtgtgaa cgatgccctt gggcttgccc gtcgagcccg aggagtagag
    17521 gatgtagagt atgtcctccg aatccatctc ctcggcaggg cactcggcgt ccgcctcgcc
    17581 cataagatca ttccagtagc ggtcccggcc ctctaccata ttcacgtcct cgccgctgcg
    17641 ctgcgcgacg acgacgctct cgatcgaagg cgtgtcttcg agggcctcgt cggcattggc
    17701 cttcaagggt gtcaccttgc cgcccctcgg cccgccgtcg gcggtgacga ggaccttcgc
    17761 ctcgcagtcg ttgatgcggt cgcgaagaga atcggccgag aaagcgccga agactaccga
    17821 gtgcggggcc ccgatgcggg cgcaggccag catcgcgatc ggaagctcgg ggatcatggg
    17881 caagtagatc gcgacggggt cgcccttctg gaccccgaga tccttgagca cgttggcgaa
    17941 cctctcgacc tcggccttca gctcggagta ggtgaaggcc ttgctatcct cgggctcgtc
    18001 accctgccac aggatcgcgg tcttgtcccc cctgccttgt tcgatctggt agtccaggca
    18061 gttgtacgcg acgttgagct ttccgccgac gaaccattgg gcctcgggcg ggtcccagtc
    18121 gagaacccga tcccactctt tgaaccagtg tagttccctg gcccgctcgg cccagaaacc
    18181 ttcgtagtct cgctcggcct cctcgtagac cgaagcgtcg tcgacgttcg cttcccgggc
    18241 gaattcttcg ggaggctcgt aagtgcgtac ctttgtctcc tccgccatct ctactccctt
    18301 cttcgcgcgt cctctgtctc tgcgccgtcc cgactgcggg tgtcgcgcga aacgtttacg
    18361 tctggcctac tctggaaacg tgtttccctc tccacgttta cccgtatact atccattatt
    18421 cctcttaccc gctcgcgccc cgattaatca ccgggtcact acctcagcgc gtcacgacta
    18481 gccttcgcgc ctctggcgac ggccccacgc attccctaga ctgtgccgga ctttgtggag
    18541 gtaagagatc aggaggcgcg atgggcggat tgctcgaagg caaaaaggta ctggttacgg
    18601 gtgtccttga ccggcgctcg atagcatact cggcggcccg gctggcccag gagcaggggg
    18661 cggagatcgt ccttagcggt ttcggccgcg ccagaagcct cacggagcga acggcaagac
    18721 gcctcgaccc agaacccccc atcctcgaac tcgacgtaaa caagccggag gacatcgaag
    18781 cggtcgccga cgacctggga gcacgctggg gggaactcga cgggatcgtc cacgccatag
    18841 ccttcgcccc cgaggacgcc ctgggaggca acttcctcac cgcacagtgg ccctccgtcg
    18901 ccaccgccct ccagacctcg gcctactcct tgaaggcgct ctccgtcggc ctcgtcgatc
    18961 tcctgggacc gggagcttcg gtcgtcggcc tggacttcga cgcccgtatc gcctggcccg
    19021 cctacgactg gatgggcgtg gccaaggccg ccctggagag cacctcgcgt tatctggctc
    19081 gcgacctcgg agggcgcaac gtcagggtca acctggtctc ggcaggcccg gtcaagaccc
    19141 tggccgcgaa aggcatcagc ggcttcgagg agatcgaggg cgagtggacg cgtcaggcgc
    19201 ccctaggctg ggacaccacc gaccccgagc ccgtcggacg ggctgtggtg agcctgctct
    19261 cggattggtg gccagcgacc acgggcgaac tcatacacgt cgacggcggc taccacgcaa
    19321 tgggggcccc gataaaatag ccgcccgcca ccagcggccg gcactcagcg acagaatccc
    19381 tcggcgaacc ccaggcccga gtcgcgacgc cacagccggg gcggtgaaag cgaacaaggc
    19441 gccagtctcc tcccgcgcat ccagcggcgt cagcaggccc cgagctcgtc cccgtggcgc
    19501 aggtgcgcgg gctccgcggg cgcgccgacc gagatcgtta ccctgttctt gtgacaaagg
    19561 gtgaccttgc caggattgcc tccaatcccc ttctttccgg catggtcggg tccgtctttg
    19621 gcgggcttgc tcttcccagg cggctcgccc gcgactcgcg atttgccgta tttcttgcca
    19681 ccccgcacgt gctccggctt gccgggctta ccggtaggcc cgcccgcctt ttcgaaactg
    19741 tcatcctccc ggatcccccc gtagctcccc gcgccggggt ctcccttgcc tggccccttc
    19801 ggttctttgc gcaccacgac agtagtctct tccgcctcct cgaccggctt ttcgacgtct
    19861 tcggacacgg ttgtctcagg aacagtagcc tccggtacgg tagcctcgcg agccgtagcc
    19921 tcgggaacgg tgcgccctgc taccgtagat tccgtctggt ctccgtcttg cgagccggac
    19981 gtgaacctgt tctccccggc aacggtgacg cccgcgaccg cgaacaggat acaggtgagg
    20041 gttgcgagta tgaccgcgcg acgcatgcgg gctcctgtct gcgagtaacg gcacggagcc
    20101 gctatcggtt tgtaccataa ggaactttat cagatccggt ccggactagc gacgggccag
    20161 ggaggcgaac ttggtgtact gctccatcca ggcgagctgg accttgcccg taggaccatt
    20221 cctgtgcttg cccacgatga cctcggcgat gcccttgtca tcggactcag agttatagta
    20281 ttcatctcga tacaaaaaca tcaccatgtc tgcgtcttgc tcgatcgctc ccgaattgtg
    20341 actgactatg ccgtctgcga gccacgaggc aggacctggc accgtaaggt cgtaaacttc
    20401 gtcttcgccg gtggggacta cctcgatgac gcgatcccag aagagatcgc tctcggcatc
    20461 ggcgagcaaa gttttgtcgt cgagcaggcc ggcgtattcg gccatcaggg tccgtgaggg
    20521 cgcaaaccgg aagcgtgacg agcctccgta ggacgtatcc ctcgccagcg acatcgcacg
    20581 gtgagacata ccacgggcgg ccatgctggc cttgaccttc tcgaagaact cgatagggag
    20641 tgtgtctacg ttggtccctg cgctccggat cagcagttga cgcgcgagct tttccgctcc
    20701 atcgcagcga ggaccgaagg cttccacacg ctttaggaag agcatctgct cgcgggcacc
    20761 ggagatggaa acggtgaaca tcgggcgatg cgcaccttgg gcaacctccc ggatccgccc
    20821 gactattccc agacggagca acaacgcggc gacatcatga gcgagtcccc tactgttggt
    20881 ggaaaagcag atcgttgagg agccacgcgt ccctgacttg cgggcgaaga tcgtgccgtc
    20941 ggttgcccac aggtgtttta gcagcacagc gatggactcg ttatcgagac caaacaccga
    21001 cacgggaact cgtttttcgt gggacctctg gttgaagatg ccaagttcgc gcagccagag
    21061 gttcacgccc gcggggcgcc agcggttgcc gttgccactc aggacgagtt ggtgccagtt
    21121 tccccgaccc gcatgacggt tgaccgtcac cccgaactcc tcggccgcag cctgggccac
    21181 gagtcggctg ttctcctctg aggcggtgca atagcgcagc ggctgaccgc tcaggtaact
    21241 accgtcacct atcaggtgac caagcaacgc gagtcgcgct tcaggccaac ccggtgcgtt
    21301 ctccggctcg ggtacatgcc gtgctatcgc gagcctgtca ccgggacgca gctcgctcac
    21361 tcggatccaa ccatcagctc caaaaagccg gtgcttggca gtcgcccgaa cactgcgccc
    21421 acttgcgagc cgaacttcga agaccggacg cctacccacg gaccaaaccc ggtccgactc
    21481 ggcctcgacg atcttcccca caggcgacat cgcaagtact cgctgccgcg ttccgaccag
    21541 ctcccgcacg ggcgtgcggt gtccgtccgc gagcaacacc agcgtgtccc cggtaacaca
    21601 gtctctcagg tccgagagga gcgggcgttt gtcgtggcgg gcttcgacgg cgcgggaaag
    21661 ctgggcaatg gcgaggacag ggacgtcgag atcgcgggcg aggactttga gtccgcggct
    21721 gatctcggcg atctcctgct ggcggttctc ggcgcggtta ccctgcccaa ccatgagttg
    21781 caagtagtcg acgacgacga gcgagagcgg catctccccg gcgacgttca gctggctggc
    21841 gaggcgcctg accttggccc gaatctccat caacgtgacg cccgccgtgt cgtcgatcca
    21901 gataggggcc ctgcttacct ctgccacccc ccttaccagc ttcggccaat cctcggcctt
    21961 gacgttgccg ctcctcaagg cctgcgcagg aatccgcgtg atctgcgaga tgagcctctg
    22021 cacgagctgc tccttgctca tctccagaga gaagatcgca acgggcatct ttttttcgcc
    22081 agacgcgtgc cagatagcgt tcaatgccat ggcagtattg tggacgcaga tgtcctgcgc
    22141 gacgaagttg gacccgtcgg gcacggtcaa gtcgtagacc tgatggtcac cgatggtctc
    22201 gatagtgacg atctcgtccc ataagagatc ctggctggcg acttgcaaca gccgctggtc
    22261 cgcgaggact tcagcgtagc ctctgaggcg ataagctggc agactgcggc ccgtgtgcgg
    22321 attatgtccg gcgtacttgc ctatcttcac ggtctcgccg gaccggcgtg ccagttccgc
    22381 gaggctgagg ctgcgttgcg cagcgacccg gcgcaccacg tcccagacct ggctcggggg
    22441 atgaccgtag ttggaacccc gcctcggtag ctcgcgacgc gtaaacgttt cgtccacgaa
    22501 gcgcagagac ttctcgccga tccagccgac ctcggcctgg tagcgtgaca cagagtccgg
    22561 agccgttatc tcgacccgcc agcaacgctc cttcttttgc cagagtttgg cgactactcc
    22621 gaagcgcgtg agcgcgtggt ggacgtcgcg cgcgagcctc tcggaacaga cggtgaactc
    22681 gatgcgcggg tacctgccca atctgtagat cgttgcatca caactgaaca acacgcgcag
    22741 aaactcagcc agatagcgtc gactccacct ccagacacag tcagggaaag atttctcctc
    22801 tgcgtacttt cccatgagcc cgagctcccg cagccacgcg gtcacagggt tgctgttgaa
    22861 cctcccacac cggtccgaac acacgagctt gtaatcgtag tgcctagaac catcgtgtac
    22921 cacctgtacc ttggggaact gacggcttat ggccccgcga aaatcttcga caagtgcggg
    22981 gtctgcgttg ctccacttcg gacacccgcc cgtcaggcct ccctcggcga taaagtaggc
    23041 caccaggcgc acacggtcga gctgccagga ttcatcgcta ccgaagatgg gcacacggcg
    23101 tggaacggcg acgtaattac cgacctcgag gtcgtgaagt ggaacccatc catcaaccgt
    23161 caagaacgga tggtgtccgg tgacctccac gctgcgcccc gtgcgggtcg tgactcggaa
    23221 gcacggcttg acgccgctat cgatccaatc ggagacggcg gccgttctta cacttcccgc
    23281 ggaagataca ttgagcaccc gcggcagccg tcgctcgacg cactcctgga tggtaagcct
    23341 ctcgccactg tcgggatcta cgaccaagct gtcccacttc aggcacttgc ccatcgccgg
    23401 tctcgcggcg agtatgatca ggtcgctctt gtggaagcct gtggtgaggc ggtcgaggtc
    23461 ttcgaaacct gtctcgacac ccgttacctc gccctcctgc tcgtagaggc gttgaatcat
    23521 ctccagggcg ccgggcgcga gctcggagac aggagcgaga cgttccctca gagtccggtt
    23581 ggagacgccg tagacgagtt gctcggccga gtcgagcgcc tcgctcacgt cctcgggctc
    23641 cctgaaagca tcctcggtga tgcggctccc gacatctatg atcgcccgca agagcgcctt
    23701 gccgcgcacg atgtcggcgt acctgcccgc gtttgcagcc gtaggcacac tctcgacgat
    23761 ctggaagacg tagggcctgc cgcctatcct ctcgaactcg ttgacgctcc tcagctcgtt
    23821 cgtgagcgtg agctggtcta tgggctcgcc gcgcgagtaa agacgcatcg tggccccgta
    23881 gatgatccta tgcacctcgg aatagaagtc ctcggcggcg agccgctccg caacgaccgc
    23941 gacagccgtc tccgaaacca gcatcgcacc aatgacggcc cgctccgcat caaggtcgtg
    24001 cggcggtacg cgggccgcct cggccccggt ctctacgctg cgcatcctcc cccggcgttc
    24061 catgacctac cagcatgcat cataaaggcc cccgttacaa gacgagagcc ttgaccttat
    24121 ggtttagatt gtgcctgtac ctgcggttga ggtgaaggcg tgtcattgta ggggcgggtt
    24181 cgatgtacgg gcgggtttca aacccgcccc tacagtttgg ggaccacgat cacctttacg
    24241 ctcgcttcga cttcgccgtg gacctggatg ggcacctggt gcgtacccag ggcctttatg
    24301 ggctcttcga ggcggatgcg acgtctgtcg aggcggatgc cgcgggccct ctcgaccgcg
    24361 tcggcgatgt tggccgccgt gacagaaccg aacaggcgct cgtcctcgcc cgttcgggct
    24421 tctatggtga tcacgctctt gttgaggata ttagagatct cctctgccct ctcggccaga
    24481 cgcctgttcc tctcctcgga ctcctccata gtgcggcggg cttcctccag cttgcccggt
    24541 gtcgccacct cggcgaggcc gcgcgggacg aggaagttgc gcacgtagcc ccggctcacg
    24601 tcgacgatgt cgccgcgctg cccgatcttc tcgacgtcct ggttcagtat tacctgcacg
    24661 gctcttacct ccccaccacg tagggaagta gcgccatctc acgggcgcgt tttatcgccg
    24721 aggcggcctc ccgctggtgc tgcggacaca ggccggtaac cctgcgggcc ctgaccttgc
    24781 cgcggtcaga gatgaaccgc cgcagcaaat tgtagtcctt gtagtcgacg taggcgacgt
    24841 tctccttgca gaatacgcaa ggcttgctct tcgtcgggcg ccccccgcgg ggcggcccgt
    24901 tccttctcct acccattcca tacctccggg tctatcaagc agtaacttcg gcgggctcgg
    24961 ggagcggctt gtgcgcgtag cccggcggga gcttcacgac catgtgcctg agcacgtcgt
    25021 cggagacgcg caggatacgc ttcaactcga cgagggtgcg ctccccggcg gtgaactcca
    25081 tgaccacgta gaaaccgtcc gtacggtggt ctatctcgta ggccagcttg cggcggcccc
    25141 agtcgttcgg cgccccggcc tcgccgcccg tgcgctcgat gatcgtacga aaacgcccga
    25201 ctgtgttctc gacctgctcc tcgtcgagct cggggatcac tatgagcatg gcttcgtaag
    25261 tgtcgatgtt tgcctcctcc ggtctagtgt cggcttcccc ggtcgatgcc agtggggaag
    25321 caggagccta ttgggttgcc aggatagtgt aacagcggga tctagctgtt acaaacagcc
    25381 ctctgagagg ggttggagtg gcgacgctga ctcgcccctg actgggtggg gatatactct
    25441 gtgcgtggag aacggagcgg gtatcgccgt cgagaccgtg ggcctggaga aggtctatgg
    25501 agagggtccg accgcggtgc gcgcccttca ggacgtctcc ctcgcgtttc cgaacggtga
    25561 gtttgcggcc atcatgggac cttcgggatc ggggaaaagc acactcttgc acatcctcgg
    25621 cgccctggac aagcctacgt cgggacgcgt ggtcgtcggg gggaccgagc tctcgggact
    25681 ctctgacaag aagctcacgc tcctcaggcg ggagcggatg ggcttcgtgt tccagttctt
    25741 caacctcatc cccacgctct ccgccgagga gaacgtgctc ttgcccgtgc tcatcgcggg
    25801 caagagaccg ggcgactacg gcgagagact ggacgagctc ctggacctcg tgaagctcac
    25861 ggcgcggcgt acgcacaggc ccgacgaact ctccggtggc gagcaacagc gggtcgccat
    25921 agcgcgggcc ctgatcaggt ttcctgacat catcctggcc gacgaaccga cggggaacct
    25981 ggattcgaag accggcgccg aggtgctcga gctgctaaga gaatcggcgg cccgcttcga
    26041 gcagaccata ctcatggtca cccacgaccc gcgcgccgca gccacggcgg gccgcgccgt
    26101 cttcctctcc gacggtcgcg tggtcgatga ggccatagac ccagaccagg acgagatcct
    26161 ggaacgcatc aagacactgg agccggacgg ataacagaat gtcagactac cgcttcagga
    26221 tgatggaaga cgaagaggcc acgcagatct ccggctggca ctacgatcct ccctacgact
    26281 tctacgacgc cacaagcgac ccggacgatc ttgaagagct gctcgacccg aagcgtagaa
    26341 aggatgccta cttctccgtc ttcgacgacg aaggagtcct agtcggcttc ttccagttcg
    26401 agagggataa caagaccgtg gacgtcgggc tcggtatgag accggacctc accggacgag
    26461 gactagggat cggatatctc cttgcaggac tcaagttcgc gagacggcgt tactctccgg
    26521 agcgcttcac gctctccgtg gcgacgttca acgagcgtgc gatcctggtc tacgaacgag
    26581 ccggcttcag gagagacacc atctacaagc acaacaccga tggcggcgag tacctcttcc
    26641 tgtcgatggt caaggaagcg tgaagctcag gcgcttctcc gggctgagct tccggaacct
    26701 gcgcgccagg gtgcaacgta ccctgctcac ggccgtcggc atcgtgctcg gggtgggtat
    26761 cgtcttcggg gtactcaccc tctcgaacac catgtcgggc actttcgcgg aactctttac
    26821 caaagcatac gggtcggccg acctcacggt tacggcggcg ggaggcggcg gcggattcga
    26881 ccagaaggcg gtcgaagagg tgcgcggcta cgagggtgtg gagtcggccg ccccgcgcta
    26941 tgcgctctct tcctcactca tcctccaaag aaatcaggaa aacgacttgc cagaggtcag
    27001 gagcatgcgt ctcttcggcg tcgagccgca gtctgcaaag ctcgcgacgg gcttcgaact
    27061 caccgacgga cacttccccc ggagtggcaa ggaactcacc ctcgacggcg gttccgccaa
    27121 gcgagcgggg ctcgggatag gcgacagggt cacggtaggt actccggaag ggccgaagga
    27181 gttgagactc gtggggctct tgcggatacc gggtggatct ttcggcggac tcgccttcgg
    27241 catggcgcct ttgcccttcg cccagaacgt cttcgacaaa cggggccaga tctccggtat
    27301 cgccgtcgag gctgaggacg gtgtctccgt ctcggacctc ggtgagagac tggacagagg
    27361 gctcaacgag ggtttgcagg ccgagcgttc cgagacgcgc acccaggaga tcagcggcca
    27421 gctccagggg ttcaagatcg cgctgctctt cttcgcgggg acctcgctct tcgtcggggc
    27481 cttcctcgtt ttcaacgccc tctccatgac catcctggag cggacgcgcg agctcgggat
    27541 gctccgcgcc ctcggctcga cacgggccat gatcgcacgc tccgtcatcg tggaggcgat
    27601 gctcctcggc gtgacaggct cgctcctcgg ggtgctcttc gggtacggga tggccagggg
    27661 gctcgtctac ctcttcggca aggctttcat gttcgagatc acatccctgg tactctcttc
    27721 tttcgcgctc gtctccgcca tagtcctcgg catcgccgtc acggtcgtcg cggccctcta
    27781 tccggccgtg agggcgggga gggtgagccc cgtagaagcg atgcggtccg ccggggccgc
    27841 cgacgaggcc gggcactcgc gcgtcgtctc gctcctggcg ccggtcctgg ggctcgcgct
    27901 cgccggcgcc gggataccct ggatctacta cctcgccaag aacctctcgg ccagcctcgg
    27961 aggcctcgtc tatgcctcgg ggatcgcggc gatcatcgcg gccttcctcg gtatctccct
    28021 ggtagtcccc gtcctcgtca ggccgctcgc ggcactgttt tctcccgcgc tcaggctcct
    28081 tttcggggtc gaggggagga tggctgcggc gaacgcgacg cgcaacaggg taaggacggc
    28141 gttgaccgct tcggccctca tggtcggcat ctcgctcatc gtggcattct cggccctcgg
    28201 agggagcctc ctcggttcga tccgggacta cctcgacggc tccctcggca gtgactacgt
    28261 agtgcagccc acccagcaga actcggacgc cggtttctcc gcacagttgc ccgagaagat
    28321 cggtcgagtt cagggcgtcg agaagacgac gagcatcgtc tcgaccttcc gccgggaagg
    28381 gaagaaggtg gacgccgtct tcggggtgga ccagaattat ccggatatct tccgcgtaga
    28441 ctacgccgcc ggagggccgg atgccttcct taggctaaag aacggcgatg ccctcgttgg
    28501 caagcagctc gccaaagacc gcaagctcga agtaggatcc aggatagggt tactcggtcc
    28561 caaaggagag aaggaatacc gggtcgaagg catcctcgag aacgacatcg tgggcggcgg
    28621 gatgggcatc tacctctccc aagagatcct tgccagcgac ttcaacgaga cccagagcga
    28681 gttcctcgcc ataaaggcca gatctggctc cgaccgggaa gccctggcgc gcaggatcga
    28741 agccgttctt gcggactacc cgcaattcac actctactcg aacgcggagt ggaaggagca
    28801 gatagagagc gacttcaacc gccagtacgt cttcttctac gccattatgg gcgtctccgt
    28861 cgccgtctcc gccttcgggg tggtcaacac cctctccatg agcgtcttcg agcggacccg
    28921 cgagatcggg atcctcaggg ccgtggggac gacgcggctc cagataggcc gtctaattat
    28981 cgatgaaggt atcgtcataa gcctcatcgg ctgcctcatc ggcgtcgcgc tcggttcgct
    29041 gctcggctac ctcttcgtcc agggctctgg cgcgggaggc ttcgagatcg acttctacta
    29101 ccccaagcta ccggcactcg cggccctgct ctcgggcctc ttcataggta tcttctccgg
    29161 cctcctccct gcccgctccg ccgcccgcaa ggacatcgtc gaggccgtcc agtacgagta
    29221 gagacaggac gactccgcgt ccgagcagat agagaggtgt cccgcatcgg cgcaaagagt
    29281 gtcgcgggct agaagtcagc cagggccttt gcgcacgcgt aaagctgaaa tgctgacgag
    29341 cgacagccaa aggctggagc gtgctgacaa ggcgccaaag gtgccgagct gacatgcttt
    29401 gtaagctcct ttacccgcat gagcggtgaa cgctgttagc atctgctacg tatactctcg
    29461 acgtactctc ggataaggaa ggtggacgct ctggcggcgt catcggccag accgaacaag
    29521 cgggtggcgg tcgtcggggc cgggatgggc ggcctggcgg cggcgatccg gctggccgcc
    29581 atgggcttcg aggtcgaggt cttcgagaag aacgcccaac caggcggacg cgtggggagg
    29641 ctggaggcgg atggcttctc cttcgatacc ggcccatcgc tcctgctgat gacggatacc
    29701 taccgcgagc tcttccgatt cgccgggcgc gatcttgacg actacgtcag gctcatgccg
    29761 ctcgacgcga actaccgcgt aacgttcggt gacggcgacg ccctgaccat ccgccgcacg
    29821 cttcccgagc tgatcggcga gctggaacgc atagagcccg gcgtcacccc gcgcttctac
    29881 cgcttcctcg aagacgcctg caacaagtac cgtctcggac gctcggagtt cgtggagcgc
    29941 gacttcgata gcgccaggga cttcttcggg ctacgcaacc tcaggctgct tctgaagacg
    30001 aaggcgctgg gcaactacta ccgccaggtc tcgaggtact tccgaaccga gaaactccgc
    30061 caggccttct ctttgcagac catgtacctc ggcctctccc ctttcgaggc gccggcggtc
    30121 tactcgctcc tcccctacac ggaactcgcc gaggatggcc tgtggttccc cgaaggcggg
    30181 atgtactccc tcgtcggggc gatggagagg cttgcggccg agctcggcgc acgcttccgc
    30241 ctgaacagcc aggtcgagcg cgtagccgtt accaagggcc gggcgcacgg cgttgtggtg
    30301 gatggagagg agatagaggc cgacgccgtg ctggccaacg cggatctccc ctacgcctac
    30361 cgcgagctcc tcgccggatc ggcggacaaa gactttctgc tgaggaggcg cgaaaagttg
    30421 cgctatacgg cctcggcgtt catgctctat ctgggcgtcg aagagaagct cgggaccatg
    30481 gtgcaccaca acttttacct ctccgggcgc tacagggaga acttcgaggc catattcagg
    30541 gaccgcacgc ttccagaaga tccttccttc tacgccgtcg tcccttcgaa gacggagccg
    30601 ggcatggccc ctgacggaat ggagagtctg ttcgtgctcg tccccgtacc gcacctcggg
    30661 ccgaacgtgg actgggaccg cgacgaagac gcgttcaagg agaaagtcta cggcctgctg
    30721 gagcggaggt gcgggctcga ccgtagccgc gtcgtctacg agaaggcgag gacgccgctc
    30781 gactggcgcg acgagtacaa cctggaggag ggggccgcgt tcggaatcgg gcacggcatc
    30841 tttcaggtag gatactttcg acctcccatg gtgtccaaag ccgtacaggg aatgtacttc
    30901 gtcggcgcga gcaccaggcc cggtaccggg gtgcctctcg ttaccatagg ggccaaactg
    30961 gtggccgacc gcataggacg cgaggtcggc ggctcttgaa gctcgacccc gcgggcggaa
    31021 agaagctcga cgagagcctc tcgctcgccg agagctacga gctgtgccgc aaggttcaga
    31081 aggcgcacag caggacctac tatttctcga ccaggttgtt tccggccgag gtcaggcccc
    31141 gcgtacacgc cctctacgcc tttatgcgct acgcggacga gatcgtcgat cacccacacg
    31201 acctgccgcc gggcgcccag ctctccgtcc tggaggagtt cgaggccgag actatggcgg
    31261 ccgtctcggg tgagagcgtc cccaaccccg ttctgagggc gtacgccgac acggtgttta
    31321 cctgtggcat agaccccgag accataaccg cgttcatgga gagcatgaag atggataccc
    31381 gcgtcttccg ctaccggacg ttctccgatc tggaggaata cacctacggg agcgccgccg
    31441 tcgtcggcct gatgatgtgc agggtcgtgg gggtggcgga agggaaagcc gaccgccacg
    31501 ccgaggcgct cggcgtcgcc atgcaactct ccaatttcct gcgcgacgtc ggggaggact
    31561 ggcgacgcgg gcgggtgtat ctgccgctgg aggatctgga gcgcttcggg tatacggagc
    31621 gggacctcgc tcttggtgtc gtagacgaac gcttcgtcgc gctgatgcgc ttcgagatag
    31681 agcgggcgcg tcgtctctac gaggacgccg acgaggggat gggttacatc ccgaggggga
    31741 gacgtttccc cgtgatcgtg gcgcgcgagc tgtacgcggc tatcctcgat cgcatcgagg
    31801 cccacaggta cgacgtcttc tgccgcaggg cgcaggtctc gcgccccgca aaactcctgg
    31861 tcgccgccaa atgcgcggcc ggcgaccccg gcgagatagc ggaccgcatt cgctacaggc
    31921 ggactgctcc ggccagataa acgtctcccg gattgcaatc cgggggctat cgtgctgtaa
    31981 ttgcccggtc accggcccca agaagagtgg gccatgacgc tcatcgagag cggcggaggg
    32041 acgggccctg agaagccgcg gcaaccggcg aggcgcgagc cccgtggcag gtgccaactc
    32101 ccgcggagga cactccggca gatgaggtga aagagcagac gcagcctctc tcctccgcga
    32161 atgtgtgacc aagaaggaga gaggttctta ccttgattcc caacttgaga gagctgctcg
    32221 ggacgcgcat gctggtcggg gacggcgccg tgggcacgtt gctcggggag cgcggcgtcg
    32281 gcttcggtca cccctacgcc cgcgcgaacc tctcccaccc ggagatggtc acggatatcc
    32341 acacggagta tctgagggcg ggcgccgacg tcatagagac gaacactttc gccgccaacc
    32401 gtttcaagct ggagaaccat gacctcgaag agagcgtgcg cgagataaac gccgagggcg
    32461 ccagattggc ccgcatggcg gcccgaaacg tcgcaggaac aggggacagg gcgctggtcc
    32521 tcggggccgt aggtccgctc ggacgtccgc tcgcccccgt gggttccgtc ggtccggacg
    32581 gggcgaagtc cgtctttgtg gagcaggccg aagcgcttct cgaaggcggg gcggacgcga
    32641 tcctcctgga gacgttcacc gacctctacg agctgcggct cgcctacgag gcggtcgaag
    32701 cccttggcgc gccggtcctg gcgtacaaga ccttcgtcga ggacggcgag acgctggccg
    32761 aagggctgcc gggtcgggcg gcgcgtgaga tctcttcctg gggcgcagac ctggcggggg
    32821 ctaactgtac cgtcggcccg caaaggatgg tcgagatcat atctcagatg gccggcgggg
    32881 ccggtcccgt cgcggccttc cccaaccccg gcctccccca gctcgtgggc ggccacatcc
    32941 gctttcaaag ggacgtagat cacttcgccg agtacggcgt aaagctcgcc gaggccgggg
    33001 cgcggctcgt gggcggctgc tgcgggacca ccccggccca cgtgagggcg ctctccaggg
    33061 cgctgcgaga cttcgaggta gcaggcaatg gagcccgccg cccggtggcc gtggtggagc
    33121 aggccagaga gaaggagata tcggcagaac ccgtctccga gttcgcggag cggttgcgca
    33181 ccggcttcgc ggtgacggtc gaggtcgacc tgccgcgcgg aaacgacatc gggcgggtcg
    33241 tagaggcggc gcgccggttg aaggagcgcg gagcccacgc gatagacatc tccgacgggg
    33301 cgcgggccag gctcaggatg caccccgttg cggcggccag gatcgtgcag gaagaggcag
    33361 ggatcgaggt ggtcgcccac atctcatgcc gcgacaggaa cattatcggg ttgcaggccg
    33421 acctgctcgg ggcagcggcg ctggatgtaa agaacatcct ggcggtgacg ggtgacccgg
    33481 cccagatcgg tgactacccg gaagcgacct cggtcttcga caccgactcg gtcggcctcg
    33541 tccatgtcct ctccaagatg aatcgcggag aagacctcgc cggcaaccct attggcgagg
    33601 cgccgggctt cctcgtcggg gcttcattca accccactgc agaagacctg gaccaggagg
    33661 tagagaagct gcggcgcaag gtcgaggccg gggcccacgc cttctggacg cagccggtct
    33721 tcgagatggg ggccctggag aacgccctgg agcgtatcgg cgacgacggg gtgtgcatcc
    33781 tgctcggcct catgcccctc cgcagcgcca ggcaggcgga gttcctccac cacgaggttc
    33841 cgggtatata cattcccgaa cacgttcgcc gaaacctcgc ctccctcccc cccgaagacg
    33901 ccccgagata cggcgtcgag gtcgcccagg agctactcgc agaggcgcag cctctggtct
    33961 gtggcgcgta cataatgccg ccggccagct ctcctgacct cgcgggggat gtgatagagg
    34021 ccatgagcct gcccgcaggg agataggagt cgatggttct ccagcacacg aagaacgaag
    34081 agaggcaggc caggttgcag gagttccggg agctgctaga gcagcgcatc gtgctcctgg
    34141 actgtgcgat ggggaccatg atccaatctt atgggctctc agaaagtgac taccgcgggg
    34201 agcgattcgc cgatcacccg ggcgagcttg ggggcaacaa cgacctgctc tccataaccc
    34261 agccggagat gatcagggcc atccacgagg cggcgatgga cgcaggatcg gacatcatcg
    34321 agacgaacac cttcacctcg acctccatcg cccaggcgga ttaccagctg gaggatttgg
    34381 cctacgaact gaactacgag gcggcccgca tcgcccgcga gaccgcggac gcctacaccg
    34441 agaagatgcc cgagaggccg cgctttgtgg ccggcgcact cgggccgacg aaccgcacgg
    34501 cctcgctctc gccggacgtc aacaacccgg gcttcaggaa catcaccttc gacgaactcg
    34561 ccgaatccta cgcagaggcc gcacgggggc tcgtggaggg tggggccgac ctgctgctcg
    34621 tagagaccgt cttcgacacg ttgaacgcca gggccgccat cttcggcatc acgtcctacc
    34681 tcgaagagga gggcttcgag gtgcccatca tgatctcggg cacgatcacg gacgcgagcg
    34741 gacgcacgct ctcggggcag gtaacggagg ctttctggaa ctccgtgcgc cacgcgaacc
    34801 ccataagcgt ggggctgaac tgcgcgctcg gatcgaagga gctgaggcag tacgtcgagg
    34861 agctctccag gatctccgac gttcccgtaa gcgcttaccc gaacgcgggg ctgcccaacg
    34921 agttcggcga gtacgaagag acaccggagt tgatggcggc cgagatccac gattgggcga
    34981 gggcaggctg gctcaacatc gtcggcggtt gctgcgggac gacgccggat cacttgcggg
    35041 ccatagccga agcggtctcc ggccacccgc cgcgcgagat accggagatc cccaaaaagc
    35101 tcaggctctc ggggctcgaa ccgtgcaaca tcggacccga ctcgctgttc gtgaacgtcg
    35161 gcgagcggac caacgtcacg ggttcggcgc ggttcaagga tctcatatcc gaaggagact
    35221 acgagacggc cctcgaagtg gcgcgacagc aggtcgagaa cggcgcccag atcatcgacg
    35281 tcaacatgga cgaggggatg ctcgaaggcg aggaggcgat ggtacgtttc ctcgacctcg
    35341 tggcctcgga gcccgacatc tcgcgggtcc ccgtaatgat cgactcctcc aggtggtcga
    35401 tcatcgaggc cggcctgaag cgggtgcagg gcaaggcggt ggtcaactcc ataagcctca
    35461 aggaagggga ggaagagttc gtcaggcagg ccttattgct gaagcgctac ggtgccgcgg
    35521 tgatcgtcat ggccttcgac gagagaggcc aggccggcac gaaggagcgg aaggtcgagg
    35581 tttgcaaacg cgcctaccgg atcctcacag aagaactccg tttcccgccc gaagacatca
    35641 tcttcgatcc caacgtcttc gccatcgcca caggcatcga ggagcacgac gactacggtg
    35701 tcgactttat cgaggcggtg cgtgagatct cctctaccct cccccactcc aagacctcgg
    35761 gcggcatctc gaacgtctcg ttctcgttca ggggcaacaa cccggtgcgg gaagccatcc
    35821 acgcggtctt cctctaccac gccataaggg cgggcctgac gatgggcata gtgaacgcgg
    35881 gccagctcgc cgtctacgac gagatagacg gagacctacg cgaggccgtc gaagacgttg
    35941 tcctgaaccg ccgacccgac tctaccgagc gcctgctcgc cctggcggag aggtaccgcg
    36001 acagggacga ggcgggatcg gacgagaagg acctacaatg gagaggctgg cccgtcgaga
    36061 agcggctcga gcacgcgctg gtcaagggca tagccgggtt cgtcgaggtg gacacggagg
    36121 aggcgcgcct ccaggccagc agcccgctcg acgtgatcga aggcccgctt atggaaggga
    36181 tgaacgtcgt aggggacctg ttcggctcgg gcaagatgtt cctaccgcag gtcgtaaaga
    36241 gtgcccgcgt gatgaagaag gccgtcgcct acctcatacc tttcatcgag gaggagaagg
    36301 ggggcgagcc tcgcaagtcc aacggcaccc tcgtaatggc taccgtcaag ggcgacgtac
    36361 acgacatcgg gaagaacatc gtcagggtcg tgcttcagtg caacaacttc gaggtcatag
    36421 acctcggcgt gatggtacct tccgcgaaga tccttcagac cgccagggag aaaggcgccg
    36481 acgccatcgg gctctccggc ctcataacgc cctctctgga cgagatggtc cacaacgcca
    36541 gtgagatgca gcgcgaaggc ttcgacattc cgttgctcat cggcggggct accacctccc
    36601 agacgcacac ggccgtcaag atcgcacccg gttacgagca gccggttatc cacgtgaagg
    36661 acgcctcgcg cgccgtcggc gtggtccaga acctggtgag cgagaaccgt agggccgctt
    36721 acgcacagca gacagccgcg gagtacgaac aggttcgcaa gaagcaccag ggacgccgct
    36781 cgaagaccac cctcgcgact ctaaagacgg cgcgcaccaa ccgcacacgg atcgactggg
    36841 aaggctacgc cccgccgaag cccaactttc tcggcgtgcg gacgttcgac gactacgcgc
    36901 tccaggagat acgtccctac atagactgga cgcccttctt ccactcctgg caactgaagg
    36961 ccagccatcc cagaatcttc gacgaccccg agaaaggccc ggaggccaga aagctcttcg
    37021 ccgacgcgca agacctgctc gaccgtatca tagccgggaa gtggctgtcg gccagcgccg
    37081 tcttcggtct cttccccgcg aacgccctgg aagatgactc catagagctc tacaccgacg
    37141 aatcgcgcga agagacgctt accagcctcc acttcctgcg ccagcaaaga cagaaaccac
    37201 caggccgtcc gaaccgctcg ctcgccgact tcgtcgcccc gaggagcacg ggtctcgaag
    37261 accacgtggg gctcttcgcc gtcacagccg gactcggggc tgacgaggtg gcgcttcgct
    37321 tcgagagaga caacgacgac ttcaacgcca tcatggtcaa ggcgctcgcc gacaggctcg
    37381 ccgaggcctt cgccgagctc atgcaccggc gggtccgtac tgagttctgg ggctacgcct
    37441 cggatgagga tctggacaac gaggccctca taagggagga gtacaacgga atccgcccgg
    37501 cccctggata tcccgcctgc ccggagcaca cggagaagcg caccctctgg agcttgctcg
    37561 acgtggagaa caatgccggc gtcacgctga ccgagaactg cgcgatgaca ccgggcgcta
    37621 cggtcagtgg ttactacttc tcacaccccg actgcacgta cttcggcgta gccgagatcg
    37681 gacgcgacca ggtcgaagac tacgccgaac gcaagggctg gaccctagag gaggccgaga
    37741 agtggctctc ccccaacctc ggctacgacc tcgaggcggg acagaagagc aacggagctt
    37801 cgagtgtcca gcaatccgtg atcaggtctg cacgctgaga ctaacggaac cgaacctcgt
    37861 cgaatcctag cgtcgcggcg aaagcgcgga tgctatcttc tgcgctccgt tctgcggtct
    37921 ctagaatctt gttctccctg gcggcgtctt cgatcttctg caccgcccgc agacgggcca
    37981 tctcgacgag attatcatcg ggacggacgt ttagggggct gaagtcgcgg tcgtagacgc
    38041 gggtcctccc ctcgtcgaga ctgacggaga ggatctccgg ttccggcaga tcgatggtga
    38101 cgctgtcgcc gtcgacggcc acgtcgtcca tttcgatgtc tccaaggtcg accccggctt
    38161 cgaccttacc ggtggctatt acgataacct tctcgccgga gaacagtcgc tccagaatat
    38221 cgccgccggt ctccctcgtg acaggcacgg actcggtcca acgcaccgtg gcgagatggt
    38281 cgagctcctc tatcccttct accaccacag gccccgtcgt ggtacgcggc ggcttctctc
    38341 cgagtagcgg cccaactatc ggcagcgagg agccaaagcg cgcgagccca acgcccagcg
    38401 cgacgcccaa tacgacgatg acgagcgcca ggacgagcgt agtaccgagc ccgccacctt
    38461 ttctacccac cacgcgcctc ccacgagagt tcccgaatgc aagattctag caccaggcgg
    38521 ccccacaggg cacaagctcc ctatcacctg aaatgttctc tacgcctgaa tattcgtctg
    38581 gcgccagcgg gtgtgctcgc cgagacctga ggcctcgggg aattcctcac ccctggagag
    38641 ctggacccgg acgggcggga gcatggttcc cctgtcgcag atgtaggtgc ccgagaaggg
    38701 ggccttctcg cctggcgtgt aggtgggccc gtagggcctg tagcgggcga gtatgtcttc
    38761 taggccggcc atcacagggt aaccgagcgg gcatcgtcga aacccggagt ctctacgagg
    38821 gcgttcagga cttcttcggg gatgggctcg tccacggtga cggccatcgc cgcgcgggac
    38881 ccgggctccc cgcgtcccac ggccatgttg ccgatgttga tgttgtgatc cccaaggata
    38941 ctgccgagct tcccgatcat gccaggccta tcctcgtttt ggatgaagag catgtacttc
    39001 tcggggacca tatcgaagtc gaagcccatc gcttccacga gacgcggctg catccttggt
    39061 ccgaagagcg ttccgctaac ggccatctcg cccatacgca actccacgag gctcgtgtag
    39121 tctgtgctct cagaagaccg cgaggtctcg accttgagcc cccgctcctt cgctagcgct
    39181 ggcgtgttga cgaagttcag tggctcgaag accatcggcg cgaggagtcc tttctgggcc
    39241 gaaacgtcga ggagacgcgt gtcgaagccg gcgacctcgc catggtactc gatcctcagg
    39301 atatcaccag cgtggtccgt gagttggtag agcagacttc ccagagactc gcacaacccc
    39361 gagaactggg ccacgaactc cgcgccctcc cccattggca cgggggcgtt tatggcgtgt
    39421 accggcacgg cgcccctgag ggccgtcgcc acctgctcgg cggccgtgat gccagccctg
    39481 tcctgggcct cagcggtgct cgccccgagg tggggcgtca ccaccacgtt ggggagcgtg
    39541 aagatagggg aatccgtcgt cggctcctcg gcgaacacgt cgagtgcggc accggcgatc
    39601 tcaccctcct tgagcgcgtt gtagagggcc gtctcgtcga cgatgccgcc cctggccacg
    39661 tttatgaggt aagcgctgct cttcatcttc gccagggcgt cctcgttcac gagatccatc
    39721 gtctgggggg tacgcgggac gtgtagcgag acgaaatccg cccgctcgaa gacctcgtcg
    39781 gcggtctgtg cccgctcgac gttcatggag cgcatcctat cctccgagac gtaggggtcg
    39841 taggcgagga cctgcatccc catacccaac gcgcccctcg ccacgatggc gccgacgtga
    39901 ccgagcccga ccaacccgat cgtcttcccc gagacctcga cacccttgag cgcgctccgc
    39961 ttccactccc caccccgcaa ggaagagtcc gcggcaggga tccgcctcgc cgccgcgagc
    40021 atgagcccca gggtgtgctc ggccgcggcg accgtgttgc tctcgggggc gttggcgacg
    40081 atgatgccgt gcttggtagc cgactctatg tctatgttgt cgacgccgat gccggcgcgt
    40141 ccgatggcct tgaggttccc ggcccgctct atcacatcgg cggtgacttg cgtggcgctg
    40201 cggacgatga ggccgtcgta atccccgatc ttctggagca gttcttcagg gccgaggtcg
    40261 aggagtacgt ccacctcgaa gtcttgctct agtaacctta tgccgggatc ggcgagcttt
    40321 tcggagacga ggaccctcaa gcctttaccc cgctcttcga gaagaccctc tgggcggcgc
    40381 cgacaccctt gccaagctcc acggggtagt ccagggactc cagcgcgagc tcggtggccg
    40441 ctatcgtggc gatgatgtcg aaagcatcga agaagccgca atgcccgatc cggaagatcc
    40501 tgcccgccat ctcaccctgc ccgccggcga cctgtatccc gtgttcgcgg aacaccaggc
    40561 gcacgagctg cttgccgtct gtaccctcgg gcacccaggc cgctgtaacg gccgagttgg
    40621 gatcctcgtc gggcccgaag agctccagcc ccatgccctt gatccccgcc ctcgaggccc
    40681 gacctagcaa tacgtggcgc tcgaagacgt tctcgacacc ctcctccaag agctgccgca
    40741 acgcgacgtc gagctggata atgagactta ccgccggggt ccatgccgtc tgggccggat
    40801 ccttctggta ggctttcctc gccgccgtcc agtcgaagta gtagcgcggc atccgggact
    40861 cttcgtgcac ccgcatggcc ctcggactaa cgctcacgaa ccccaggcca ggcggcgtca
    40921 tgagcgcctt ctgagagccg gcgaccacga cgtcgattcc ccactcgtcg gtcctcaacg
    40981 tacaggcccc cagccccgag accgcatcca cgatcgtgac gacgttctgc gcggccctgg
    41041 cgaagccttc gatgtcgttt acggtgcccg tcgaggtctc cgagagcacg cagattgcgg
    41101 ctttgatccc ggggtcgccc gccagcgctt ctgcgacctc gtcgttgtcc gccttctgac
    41161 cccactcgta ctgaagctcg gtgacctcca ggccgaaggc ccggcccatc ttgacccacc
    41221 ggccgccgaa gttaccgttg ttgacgacaa gcaccttgtc ccccggcgaa aacaggttct
    41281 ggatagcacc ctcgaaagcc ccggtcccgc tcgccgcgta ggtaaagata tcgttctccg
    41341 tcaagaagac ccgcttcaag ttctcgttca cccgggtgaa cacctcgccg aactccggcg
    41401 tgcggtggtg aatgatgggt agagcccccg ccgctgagac ctcgggcggt atgggagtgg
    41461 ggcccggaga cataagacgg tacttgttca tcatggatgc tgagcttcct ctcttaccgc
    41521 gtataacagg acttccattt tagacattat cgttgccgat ggctacatga tgggacatct
    41581 cttttacaaa gtgttgctat cagcatttca gctagtcagc acgtcgcccg gcttcgccgg
    41641 gcgatttgtc agcacttcag cacgtcagcc aggggttgcg caggcgaaag ctgaaatgct
    41701 gacaagcgac agccgcaggc tggagcgtgc tgactagctg aaatgcgcgg gtttaggaag
    41761 ggtcgaccct tcctaaaccc actggaaggt ctttgttctg gctgactcgc gggcggcgag
    41821 gcacatggcg gcgctgagaa gcccatctct cagcgaagcg ttgggactgg cgcgtctctt
    41881 taccaggtcc aggaagccct cggcgaggat gaggtctccg ccggcgtgcc cgtcatcctc
    41941 tacatccacc cgcatctgct cgaccttctc ggagcggtgg tggatgatcc gcagttccat
    42001 cagggccagg tcgaattcca gtgttgcttc gtatcctatg accgtggcgc cacgtttgta
    42061 tgccgagcgt cgtgagagga agttctggct gtagctggcg tggatgccgt tctcgtacat
    42121 gatgagcgcg gagccggcat catggttctg tatctcgcgg ctgaaagcgc accagtggtc
    42181 ggtcctgtcg tcgctgtcct ggtacatccc gccgtcgtct cccctgaggg cgaggttctg
    42241 gggactctcg gggcacgttt cggtctcgtc gcaggccgaa catctgaggt cgtgaggttt
    42301 ctctccgccg tagaccctgc gcgactccat cgcggcgatt aaggcgggac ggcttccggc
    42361 gagcaggttg atgcagtcga aatcgtgggt ggctttctgc agccagaggc cgcccgtctg
    42421 gtcgtggttt ctgtaccact ggccgaagta caccccgccg taaggcacgt tgtttacggc
    42481 ctggatctgg tttatcgtcc ccagtgcgcc ggatctcacg acctccagca ccttcgagaa
    42541 caggggcgtg tggcgcaagg ggaaggagac tgcgacctca tcctccctgc cccggaatgc
    42601 ctcccgcagc gcccccaact cctccgggct aatggcgacc ggtttctcca ggaagagcgg
    42661 cagcccaaca gaggcgacct tgatggccat ccgggtgtgc aggtggcacc tggtcccgat
    42721 cacgacgccg tcgtagtcgg cagggtcttc gagcatggcg tcagccgaag gatataaccg
    42781 caccccggca ttaccgacca ttgcgagccg ccggccggcg ccttcggtgt cggggtcggc
    42841 gacggcccgg acactcacgc cggggtcgac ggcctgcatc gaggcgatca tcgcggtggc
    42901 ccggtgaccg agcccgatca cagcgagcct cgtcaagcct gctggatcag gcaccacccg
    42961 ctccgcatga tgaggagagg catactgggg gttttctcac cgaactactg tccggcgggt
    43021 ttgagggtgc cgtccatgat catcatgacc gtgaagctgt cttcgtaggg actgttcacg
    43081 tagatggggt tgctatctgg caaccagccc gtaacccgtt cgccatcgag cgccgggttg
    43141 ttgccgtagc gctcccagag cgggatgaca ggtaggagct cgttgaagat cttggagagt
    43201 ttggtcacgg tcgccttctg gtcctctacg tcgagccccg aggcggtctg gatgatcagc
    43261 tcctccagg
//
LOCUS       DATMVF010000127        20575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_12703, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000127 DATMVF010000000
VERSION     DATMVF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 20575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20575
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12703"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1749
                     /locus_tag="VN178_06475"
     CDS             97..1749
                     /locus_tag="VN178_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWS80654.1"
                     /translation="MSGAILVAGTHSDAGKSVVVAGICRWLAREGASVAPFKAQNMAL
                     NSFVTREGAEIGRAQASQASAAMVEPEVSMNPILLKPSAERTTQVVVRGKPWANASAK
                     SYGAMKKALVPVVLESLEELRGRFDVVVCEGAGSPAEINLRENDLANMGLARAADLPV
                     LLVGDIDRGGLFASLYGTLALLSAEDQSLVSGFLVNKFRGDPAVLAPGLERMTSLTGR
                     PFLGTLPWIPGLGLDGEDSLTLDTPLMAKPPLGRDVLKVAVARFGRISNFTDFDALSH
                     EPGVSVRFTESAEEILDADLAVLPGTKATVEDLRLLRSRGIDHVFAERAKRALPTLGI
                     CGGYQMLGGRIEDGVESSEAEVLGLGLLPVETCFEAEKVLGRPEGRAVGFGDAEVSGY
                     EIHHGRVRRDGGDPLFETREDTEGCRIGATLGTSWHGVLESDGFRRALLQWVAGERGL
                     DWKPGEDSFAAARESQLEKLGDLVADHVDREAFMRLIDEGAPRDFPVFRLQATGFRLQ
                     EGEGEASDTQAVSGSNGDGPLTGPAEPANLPATTPKDLKPDA"
     gene            1742..5491
                     /gene="cobN"
                     /locus_tag="VN178_06480"
     CDS             1742..5491
                     /gene="cobN"
                     /locus_tag="VN178_06480"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HWS80655.1"
                     /translation="MPETLNSKLLFVTTADTEILAAARASERMPERFPRLLCANPTVL
                     EDPTAFFERELPGTAAVLVRLLGGRRAWPEGLEELRRQGARLKVPLLAFGGEAEPDAE
                     LAALSTVPSGTVLEAFEYLRHGGVRNTENLLRFVADTVLMEGYGFEPASPLPEVGVYH
                     PGLREGSTVEELLARHDPARPTVGVIFYRAHWMSGNTAFVDALVEALEAAGADALPVY
                     CYSLRAGPEGGVPALELLKDRVDCLITTVLAGGGSNAADARRSGSPEEWLEWDVPALE
                     ELGVPVIQGICTTSTREGWVVSDAGLSPLDTAWQVAIPEFDGRIIGVPFSFKERLGEA
                     SPVGAPLTLYKADLERTARLAELAARFARLGRIPNSEKRVAVLLSNYPTKHSRVGNAV
                     GLDTPASAIGLLDAMREAGYVVEDVPVRGDDLIHSLIAAGGHDLEFLTGDQLGNAAGR
                     LDAERYAEWFGRLPEELRSDVEEHWGRPPGDLYVDDGDLVVAGLSFGGVFVGIQPPRG
                     FGENPIAIYHDPDLPPTHHYLAAYWWMIEEFGADAIVHLGKHGTLEWLPGKSLGLSSA
                     CAPDAALRDVPLFYPFVVNDPGEGTQAKRRAHATVVDHLIPPMTRAETYDDLAKLEQL
                     LDEYYQVETLDPSKLPAIRVRIWETLRDAELHRDLGVEEQPEEFSDFLTHVDGYLCEI
                     KDLPIRGGLHILGETPEGEPLRHLLASILRLGAGEVPGLRRAVGAAFGLDERVLAENG
                     GTKVEAPPGFVERFPGTVVTASDLLDRLEEAQQALLRGMEERGWDAAGAVCQETLGFA
                     DTGVERALSFASEEVVPRLMRTPEEMGNLLSGLGGGYVPAGPSGSPTRGLMNVLPTGR
                     NFYSVDPKALPSALSWEVGRGLADDLLRRYLEEEGRYPETVGIVVWGTAAMRTQGDDI
                     AEILALLGVRPVWNEESRRVVGLEVIPLTELGRPRIDVTVRISGFFRDAFPNLISLLD
                     EAFTTVANLDEPEDMNFVKKHAGEERSRGADERRSTTRIFGSKPGAYGAGLLPLMDAR
                     NWRTDEDLAEVYAVWGGYAYGRGLDGVEAREAMESNLRRTEVAVKNVDNREHDLFDSD
                     DYFQYHGGMIAAVRALTGRDPKAYIGDSADPSRVKTRDLSEEARRVFRSRVANPKWIT
                     TMQRHGYKGAFELSATVDYLFGYDATAGVVEDWMYSDVTRKYVLDHEVRDFMQQSNPW
                     ALRAISERLLEAAQRSLWSEPDPEDLEALKQVYLENEGMLEEGSP"
     gene            5488..7437
                     /locus_tag="VN178_06485"
     CDS             5488..7437
                     /locus_tag="VN178_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative cobaltochelatase"
                     /protein_id="HWS80656.1"
                     /translation="MNAYRHPYPFSAIVGQEDLKLALLVNAVSPEVGGVLVRGEKGTA
                     KSTAVRALAGLLPGIRVVSGCPYSCDPDSPDPGCPAGPHPSDASAETRPVRLVELPVG
                     ASTDRLAGTLDIEKALSEGRKAFEPGLLAAAHRGILYVDEVNLLSDHLVDLLLDVAAM
                     GVNYVEREGVSVRHPSRFILVGTMNPEEGELRPQLLDRFGVTVEVSGSPDPADRVEVV
                     RRRLRYEAAPEEFSGEWSNADGKLAASVESARMRLPQVELDEDILFRISALCAELDVD
                     GLRGDLVTAKTALTLAAWDDRNEVVLDDVKRAALLALSHRRRRGPFEQPGIDPEELEN
                     ALAEDPDPPDGPDGGMAPPSMSEDDGEASSTVRDPEPSEARTGAGERSHAASEPFDPV
                     RLEVTEKGRGGPLGRRSRISGEAGHPVADREPKENPRDVALAATVRAAAPHQRARGRE
                     GPGIEVRTCDLRQNVREGREGNLILFLVDASGSMAARKRMSAVKGAVLSLLNDAYQRR
                     DKVALISFRGEGANILLPPTSSVALAAPRLQDLPTGGRTPLAAGIEKAAEALEREELR
                     DGERRPLLVLLTDGKATAGSDPRVAAARLRSLTATSFVVDTEEGYVRLGMAGELAEAM
                     GARCLRLEELRAEALVELVERRRVA"
     gene            7434..8006
                     /gene="cobO"
                     /locus_tag="VN178_06490"
     CDS             7434..8006
                     /gene="cobO"
                     /locus_tag="VN178_06490"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWS80657.1"
                     /translation="MSETREPREQRLRRRSSRERPLLIVNTGHGKGKSTASFGVMLRG
                     WARGYRIGVYQFVKSGKWNVGEQKAAEALGNIDWFKQGDGWTWTVKDLEHSADLAREG
                     WEEVKRRLADETYDMLLLDEFTYPMKFGWVDTSEVVEVLKNRPGFQHVIITGRDAPPE
                     LVEISDLVSEVNKIKHPFDEGYRGQKGIEW"
     gene            8000..9361
                     /locus_tag="VN178_06495"
     CDS             8000..9361
                     /locus_tag="VN178_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HWS80658.1"
                     /translation="MVDTLRIVVAGTHSGVGKTTITSGLMAALSSSGFRVAPFKVGPD
                     FIDPSYHTLAADRPGRNLDAFLSGPELIGPLFAHGAQGADIAVIEGVMGLFDGKGGGD
                     LASTAHVARLLEAPIVLVVDARAMSRSVAAVVHGYATFDPKVRVAGVVLNRVGSTSHE
                     AMLREAIKPLGIPVLGVLRRDDQIQTPDRHLGLVPVAERREEAARSLDVLGMMISRSL
                     NLDGIVRLARSAGSLEVEAWSPETRDLDRYEVARVAVATGPSFSFLYRENMELLQGAG
                     AEVVTFDPTTDEALPAGTDALYLGGGFPETYAEALSMNGRMRENVRSFAESGRPVVAE
                     CGGLLYLVRELDGYPMCGVLDAEATMMDRLTLGYREARALMDSPLAEHGAEVRGHEFH
                     YSAVEPGAGEKPAWELVGRGQEGFVNGGVHASYLHTHWAATPELPRRLVQEATRSATG
                     VGV"
     gene            9358..10071
                     /gene="cobI"
                     /locus_tag="VN178_06500"
     CDS             9358..10071
                     /gene="cobI"
                     /locus_tag="VN178_06500"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HWS80659.1"
                     /translation="MSGRLVGVGVGPGDPELLTVKGLRVLREADEVFVPVADTGEVGR
                     AEATVREYVDEGRIKRLLFALSDDLEARARNWTNAAQDVSEHLRGGKTCAFATIGDPN
                     VYSTFTYLARAVREIEPDVQVETVPGITALQDLASRSGTVLLEGDERLALLPFTAGEE
                     PLREALAGFETVVCYKGGSRLGGVLRVAEEGGRLEGAVYGARLGIEGEEVVPALEMVG
                     REGTYLSTVIFTGRSSELG"
     gene            10073..10852
                     /gene="cobM"
                     /locus_tag="VN178_06505"
     CDS             10073..10852
                     /gene="cobM"
                     /locus_tag="VN178_06505"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HWS80660.1"
                     /translation="MWFIGAGPGAPDLLTVRGARLIGEADVVIWARSLVQEGVLQYAR
                     PGAVIVESTTIPLEGVRELYQRAVDEDSRVARVHSGDPSLYGAVMEQIELCEEIGLDW
                     EMVPGVSSLGAAAAAIGRELTVPEISQSVILTRRASRTPMPEGEEIRSFAEHGTTMAI
                     FLSAAKPRVLQQELLEGGYAPETPCAVVYRASWPDEEVIECRLEDLADRVREAGFTRQ
                     ALILVGPGLGAGGTRSHLYSPVFSHMFRRANPAQKGADAAT"
     gene            10839..12005
                     /locus_tag="VN178_06510"
     CDS             10839..12005
                     /locus_tag="VN178_06510"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HWS80661.1"
                     /translation="MPPRKLREPSMPPSMEKVKGQRLRTGWTTGTCAAAAAKAAASAL
                     VSGRRQARVDVKLPGKGRERRVEFGVERCDLGASWAEAVVIKDAGDDPDVTDGAHLTA
                     HISWREEQGFELDRGDGVGLVTKPGLGLPVGGPAINAVPRRMISYSVEEVLDPNERGV
                     RVVISVPGGEKMAEKTTNARLGIVGGISILGTTGIVRPFSTAAWAASVVQAIDVMGAQ
                     GHNTFVLSTGGLTEKAAMRLLPDLEEVNFVEVGDFTGQALKKAVENSLERCFFVGMVG
                     KISKLAAGVMMTHWTRSKVDNELLARITLEAGGPTNLVEEVKEANTARHAYELWRSAN
                     LERASDLLCKQAAENLAEYTQGKLEVHVILVDFDTLEPAGASPGALVLTAWGPV"
     gene            12002..13210
                     /gene="cbiE"
                     /locus_tag="VN178_06515"
     CDS             12002..13210
                     /gene="cbiE"
                     /locus_tag="VN178_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HWS80662.1"
                     /translation="MNRGRITVVGLDGRPLGEEIERLLADAALVAGGGRHLEMFEVDR
                     DRAVVLKGDLSEAFVRIEDTEGPVVVLASGDPGFFGIVRLLGERFGRETLRVLPGLSS
                     VALVFARAGLSWNDAVTVSAHGRDPHRAVNVCRTHPKVAVLTSPDFGPAELAEALGGL
                     DRTFVVAEKLGEADERVFQGDAGTVAGKEWKEPNVVLVFDEVRMPGARGWISSSPQSP
                     SRWALSEEEFEHRSGMITKPATRAFVLSRLGPGPGDLIWDIGAGSGSVAIECARLGAA
                     VIAIERDPESCALISRNAEHHGVRLQIMEAPAPGVMLDLPEPDAVFIGGTGGAFEEIV
                     KLSAVRARRAVVLALVTLERVVPAAQLLEDRGLEVESTFLQASRMKGVGNLHRLAAES
                     AVFVISGSRQ"
     gene            13207..14892
                     /gene="cobJ"
                     /locus_tag="VN178_06520"
     CDS             13207..14892
                     /gene="cobJ"
                     /locus_tag="VN178_06520"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HWS80663.1"
                     /translation="MIGLLAVTANGRRNAAHLAGAWPDARLYEGKAKEVLHRAWNECD
                     GIVLFLATGAAVRLVAPLLESKHRDPGVVTVDDAGNFAVALCGGHEGGANVLAVRVAE
                     TLGGTPVITTASDSVGVSALDSLVEKIGLRLEGGSDLASVGAALVSGERVSLVTDRRF
                     PLGPLPENVVRTEQWEAPMILISDRLVETLRPAVVYRPPSLVAGVGCSRGAGAEEILD
                     LLRRSLAQAGLAEKSVAALASIDVKRDEAGLLEAAEKLDTPIHFHPAEALSIVKAPNP
                     SEVVREAVGTPSVAEAAVVSCGAELVLQKRKSANATVAIGRLPVRGRLALVSLGPGED
                     TLIPPLAREALADSEIVIGLDQYVDRIRHLLRPGTRVLTPPLGNEVERAELALGEARA
                     GGNVALVSSGDVGIYAMASPALELAGEDVDVVVVPGVTAAQAAASLLGSPLGHDHCSV
                     SLSDLLTPWGVIQDRVRAAAEGDFVVSLYNPRSKGRDWQLGKAKEILLEHRPPDTPVG
                     IVKNAYRPTQQVIITDLASLLPEDVDMLTIVLVGSSQTRVVAGRMVTPRGYLW"
     gene            14889..15092
                     /locus_tag="VN178_06525"
     CDS             14889..15092
                     /locus_tag="VN178_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWS80664.1"
                     /translation="MKAVFAVTDVCAGCGACLKTCPEKAIRPAPRDHGAPLLVLEDRC
                     SGCAECAEVCPVSACVEILLEAE"
     gene            15089..15673
                     /locus_tag="VN178_06530"
     CDS             15089..15673
                     /locus_tag="VN178_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HWS80665.1"
                     /translation="MRRIHPIETESYRILREIVDLSHLGPLSRAVAERVIHASADLDY
                     AENLVLDEAALEGGISALRRGEPVVTDVGMVAAGITGRQTLCFVSDPRAQALSEQPGI
                     TRSAAGFRVAAEEVGNGAVWVVGNAPTALFELLGLDVAPSLVIGLPVGFVGAAESKSA
                     LLHSGLPAVANRGPKGGSAVAAAALNALIYCEEG"
     gene            15675..16598
                     /locus_tag="VN178_06535"
     CDS             15675..16598
                     /locus_tag="VN178_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HWS80666.1"
                     /translation="MRRMVSTENGTYENGDAPALLIIGHGSRDPRGAKEFHELVDLVR
                     RRNPSLTVEGGFIELSRPPISECVDQLAERGARNVAAVPLMLLAAGHAKDDIPATLVR
                     EKMGHPEMSFSYGRALGIRPELLELMEERISAVVHEEEKEETAVLVVGRGSSDPDANS
                     DLSKIARLFYEGRPYPVVESAYVSMTPPDVANGLDRCLKLGAKRVVVFSYFLFTGVLE
                     ERIRGQSRAFAEGNPGVEALYAGYFGPDPSLADLVVERYTEALRGDIRMNCDVCVHRV
                     ALPGFEEKVGAPATPHYHPDEPGHQQHGHHH"
     gene            16619..17659
                     /gene="cobT"
                     /locus_tag="VN178_06540"
     CDS             16619..17659
                     /gene="cobT"
                     /locus_tag="VN178_06540"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWS80667.1"
                     /translation="MLELGEGIPPPDEAAAEEARRRHIGLTKPPGSLGRLEELGARLA
                     GMAGERPPAPESPAVVVCAGDHGVLERGISPWSQEVTAAMVGNFCTGGAAVNGVAKTV
                     GARVSVLDVGVAGELERHPLLRAAKVRGGTNDLSRGPAMTREEAARAVMAGAGVVEEL
                     VESGGVDLLVTGDMGIGNTTPAACLISAFTGSLPQETTGRGTGIDDQTLKLKVNVVEE
                     ALELHEPDPEDPLGVLAQVGGLEHAAVVGVILAGAVYRLPVILDGVVSNSAALVARAL
                     APHSAGYIIAGHRSAEPGASISLRSLKLPPLLDLDMRLGEGTGGLLAVPLVQAAARTL
                     AEMATLEELGIG"
     gene            17652..18461
                     /locus_tag="VN178_06545"
     CDS             17652..18461
                     /locus_tag="VN178_06545"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWS80668.1"
                     /translation="MVEPLNQGAIESLNSPLAQVVEVHGALDSTQERARELARAGSPN
                     GTLVVAEVQTGGRGRLGRHWGSPIGGLWMSLMLRPRFAASLASRITQTAAVGVAKAVW
                     EVGVEVRIKWPNDLLAGGKKVCGILAESSAGNAGETVEERRLDYVILGVGMNANLDPA
                     ELGVQNREVTTIRAELGRDVDLLELLRALLSSLDAELGRIEDFAAVLEDWRNLNCTLG
                     EKVRVRRFGETVEGRAVDLSREGALLLSTREGTVELFEGEIEHLRQGVDIL"
     gene            complement(18513..18767)
                     /locus_tag="VN178_06550"
     CDS             complement(18513..18767)
                     /locus_tag="VN178_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80669.1"
                     /translation="MAYFEGQLKRASTEAGEEHTGYVLETEIDMSAVQDADELVGDQI
                     AVTGDVEIVDFPERGKILVFRATSVAVLEEDEEDEEMPTG"
     gene            19061..19849
                     /locus_tag="VN178_06555"
     CDS             19061..19849
                     /locus_tag="VN178_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80670.1"
                     /translation="MSATLMYVGIGAVLATCVAILTIAVLFLRTARRYVDLTERRLEL
                     LHEREALLLALVQRQGHAPEESQEREVERREKVPEMVGYTRGRRQDEASPGSRRPEGL
                     DRHVRGTPYRGSSSEVEDEEERTPKEDPEPARPKEGSPRLGVQVPHPDDDVPGRGWNK
                     APARFFQKCYDRYLEHYEGYVRLAERLHQMRDGVDAAPGSLVKREWENKLRRAYDAID
                     RTTQRLDMLEEHYPELATDNDRISSRIGTARLHAGLTERFGRPQ"
     gene            complement(20127..>20575)
                     /locus_tag="VN178_06560"
     CDS             complement(20127..>20575)
                     /locus_tag="VN178_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWS80671.1"
                     /translation="GEGWRRRARTWSARLISRLGEELQAARRQLAARPSEVPKLAALA
                     LGYWAFDALCLILMFDALGVAADPFVLLVAYGVATTIAAIPLTPGGIGIFEVTMLATL
                     ALLGVGPEAAIPILGYRLFNFWLPIPLALVFYPTLRCNGRGRDPHG"
BASE COUNT         3615 a         6491 c         7054 g         3415 t
ORIGIN      
        1 cacgcaggtg gccttctggg ccggtctcgg actcctcttc ggggtgttgt gcgagcgtgc
       61 caggaagaag ggcgtgtcgg agaagggcgt gtcggcttga gcggcgccat actcgttgcg
      121 gggacccact ccgacgcggg gaagagcgtc gtcgtggccg ggatctgtcg ctggctcgcg
      181 cgcgagggtg cgagcgtcgc gccgttcaag gcccagaaca tggccctcaa ctccttcgtg
      241 acgcgggagg gcgctgagat cggacgcgcc caggcctccc aggcctccgc agcgatggtc
      301 gaacccgagg tgagcatgaa cccgatcctt ctcaagccct ccgccgagcg gaccacgcag
      361 gtcgtcgtgc gcggcaagcc ctgggcaaac gcgagcgcca agagctacgg ggcgatgaag
      421 aaggcgcttg tgcccgtggt gctcgagtcg ctcgaagaac tgcgagggcg tttcgacgtt
      481 gtcgtgtgcg aaggagccgg aagtccggcg gagatcaacc tcagggagaa cgacctggcc
      541 aacatgggcc tcgcgcgcgc cgccgacctg cccgtcctcc tcgtcgggga catcgaccgc
      601 ggcggcctct tcgcgagcct ctacgggacg ctcgcgcttc tatcggccga agaccagtcg
      661 ctcgtctccg gcttcctcgt caacaagttc aggggggatc cggcggtcct ggcacccggt
      721 ctggagcgga tgacctcttt gacaggccgc ccctttctcg ggacgctgcc ctggatcccc
      781 ggtctcggcc tcgacggtga agattccctg actctcgaca ccccgctcat ggcaaaacct
      841 ccacttggcc gagacgttct aaaggtcgcc gtcgcccgct tcgggcgcat cagcaacttc
      901 acggacttcg acgccctgtc ccacgagccg ggcgtctccg tacgcttcac cgagtcggcc
      961 gaggagatac tcgatgctga cctcgccgtc ctgccaggaa ccaaagcgac ggtcgaggac
     1021 ctgagactgc tccgctcgcg cggaatcgac cacgtcttcg ccgaaagagc gaagcgtgcc
     1081 ctgcccaccc tgggcatctg cggcggctac cagatgctcg gagggcgcat cgaggacggt
     1141 gtggagagta gcgaggcaga agtcctgggc ctcgggctcc tgcccgtcga gacctgtttc
     1201 gaagccgaga aagtcctggg gcgtcccgag ggacgggcag tcggattcgg ggacgcggaa
     1261 gtctcggggt atgagataca ccacggaagg gtcaggcgag acggtggcga cccgctcttc
     1321 gagacccgtg aagataccga gggctgccgg atcggcgcga cgctcggcac gagttggcac
     1381 ggcgtcctcg agtcagacgg cttccgccgc gccctgctgc aatgggtggc cggagaacgc
     1441 ggcctcgact ggaagcctgg cgaggattcg ttcgccgcag ctcgcgaatc gcaactcgag
     1501 aaactcggcg atctggtagc ggatcacgta gaccgtgaag cgtttatgag gttgatagac
     1561 gagggtgctc cccgcgactt ccccgttttc aggcttcagg ccacaggctt caggcttcag
     1621 gagggggaag gcgaagcgtc cgatacgcag gcagtgtcag ggtcgaacgg cgacggtcca
     1681 ttgaccgggc ctgcagagcc tgcgaatctg cccgcgacca caccaaaaga cctgaaacct
     1741 gatgcctgaa accttaaatt ccaagctgct tttcgtcacc accgccgaca cggagatcct
     1801 ggccgccgcc agagcttctg agcgtatgcc cgaacgtttc cccaggctcc tttgcgccaa
     1861 ccctaccgtt ctggaagacc ccacggcgtt cttcgagagg gagctgcctg ggacagcggc
     1921 cgtcttggtg cggctgctcg gcggacggag ggcctggcca gagggtctcg aggagttgcg
     1981 gcggcagggc gcgcggctga aggttccgtt gctcgccttc ggcggggagg ccgagccaga
     2041 cgcggagctg gccgcgctct cgaccgttcc ttcgggtacc gtgctggagg ctttcgagta
     2101 cctgcgccat ggcggcgtcc gcaataccga gaacctgctc cgcttcgtcg ccgacaccgt
     2161 cctcatggag ggctacggct tcgagcccgc gtcgccgctg cccgaggtgg gcgtctacca
     2221 tccggggtta agggagggct ccacggtcga agaactgctc gcccggcacg acccggcgcg
     2281 gcctacggtg ggcgttatct tctaccgcgc gcactggatg agcgggaaca cggccttcgt
     2341 ggacgcgctg gtcgaggcgc tggaggcggc cggggccgac gccctgcccg tctactgcta
     2401 ctcgttgcgc gctggtcccg agggtggggt accggctctg gagcttctga aagacagagt
     2461 agactgcctc atcaccaccg tcctcgccgg cggagggtcc aacgcggcgg acgccaggag
     2521 atccggctct cccgaggagt ggctggagtg ggatgtcccg gctctcgaag agctcggtgt
     2581 gccagttata cagggcatct gcacgacctc gacgcgggag ggctgggtcg tctccgatgc
     2641 cggcctctcc ccgctcgaca cggcctggca ggtggccata ccggagttcg acgggcgcat
     2701 catcggggtc cccttctcgt tcaaggaacg cctcggtgaa gcctcgcccg tcggcgcccc
     2761 gctcacgctc tacaaagccg acctcgaacg caccgcccgt ctcgccgaac tcgcggcgcg
     2821 cttcgccaga ctcgggcgta taccgaactc ggagaagagg gtcgccgtgc tgctctccaa
     2881 ctacccgacc aagcactcgc gggtgggtaa tgctgtggga ctggataccc cggcgagcgc
     2941 catcgggttg ctggacgcga tgcgggaggc gggctatgtc gtggaggatg tgccggtgag
     3001 gggagacgat ctcatccact cgctcatcgc cgccgggggc cacgatctcg agttcctgac
     3061 cggggatcag ctcgggaacg ccgccggccg cctggatgcg gaacgctacg cggagtggtt
     3121 cggccggctg ccggaggaac tgcggagcga cgtcgaggag cactggggga ggccgccggg
     3181 ggatctgtac gtggacgatg gagaccttgt tgtggctggg ctctctttcg ggggcgtctt
     3241 cgtggggatt cagcccccga ggggcttcgg ggagaacccg atcgcgatct accacgaccc
     3301 cgatctgccg ccgacgcacc actacctcgc cgcctactgg tggatgatcg aggagttcgg
     3361 cgccgacgct atagtccacc tcggcaagca cggcaccctg gagtggctgc ccgggaaatc
     3421 tctcggtctc tcgtccgcgt gcgcccccga tgccgccctg cgtgacgtgc cactcttcta
     3481 tccctttgtc gtaaacgacc ccggtgaagg gacgcaggcc aaaaggaggg cacacgcgac
     3541 cgtcgtggac cacctgatcc cacccatgac ccgcgccgag acgtacgacg atctggcgaa
     3601 gctcgagcaa ctcctggacg agtattacca ggtggagacg ctggacccct cgaagctgcc
     3661 ggcgataagg gtgcgtatat gggagaccct gcgcgatgcg gagctccacc gcgacctcgg
     3721 cgtggaggag cagcccgagg agttctcgga cttcctcacc cacgtcgacg gatatctctg
     3781 tgagataaag gatctgccca tacggggagg cctccacatt ctcggcgaga cgcctgaagg
     3841 cgagcccctg cgtcacctcc tcgcctcgat cctgcgcctc ggcgccggtg aagtacccgg
     3901 attgcgccgc gctgtaggcg ccgccttcgg cctcgacgag agagttcttg cggagaacgg
     3961 cggcacgaag gtcgaagctc cgcccggatt cgtcgagcgc ttccccggta ccgtggtaac
     4021 ggcctctgat ctcctcgaca ggctggaaga ggcgcagcag gccctgctgc gcggaatgga
     4081 agagcgtggc tgggacgccg caggcgcggt gtgccaggag acgctaggct tcgccgacac
     4141 cggcgtggag cgagctttga gctttgcatc cgaggaggta gttccgcgac tgatgcgcac
     4201 gcccgaagag atggggaacc tgctctccgg actcggggga ggatacgtgc ctgcggggcc
     4261 atccggatcc ccgacgcgcg gcctgatgaa cgtgctcccg acggggcgca acttctactc
     4321 cgtggacccg aaggcgttgc cgagcgcgct ctcgtgggag gtcggacggg ggctcgcgga
     4381 cgacctgctg cggcgctatc tcgaagaaga gggccgctat ccggagacgg tcggcatcgt
     4441 ggtgtggggg acggcggcca tgagaaccca gggcgacgat attgcggaga tcctggctct
     4501 actcggcgtg cgaccagtct ggaacgagga gtcgcggcgg gttgtcggac tcgaggtgat
     4561 cccattaaca gagctgggac gaccgaggat cgacgttacg gtcaggatca gcggcttctt
     4621 cagggacgct tttccgaacc tgatctccct cctcgacgag gccttcacca cggtcgcgaa
     4681 tctcgacgag cccgaagaca tgaacttcgt caaaaagcac gccggcgagg aaaggagccg
     4741 gggcgccgac gaacgccgct cgaccacgcg catcttcggg tccaaaccag gcgcctacgg
     4801 cgcaggcctg ctccccctga tggacgcccg caactggcgt accgatgagg acctggccga
     4861 ggtctacgcc gtctggggag ggtacgccta tggtagaggg ttagacggtg tcgaggcgcg
     4921 ggaggccatg gagtccaacc tgcgccggac ggaggtcgcc gtaaagaacg tagacaaccg
     4981 cgagcacgat ctcttcgact ccgacgacta cttccagtac cacgggggta tgatcgccgc
     5041 cgtccgcgcc cttacgggcc gcgacccgaa ggcgtacatc ggagactccg cagacccctc
     5101 gagggtgaag acgagggacc tctccgaaga ggcccgccgc gtcttccgga gccgcgtagc
     5161 caacccgaag tggataacga ccatgcagcg ccacggctac aaaggcgcct tcgagctctc
     5221 ggccaccgtg gactacctct tcggctacga tgccacggca ggcgtggtcg aggattggat
     5281 gtatagcgac gtaaccagga agtacgtcct cgaccacgag gtaagagact tcatgcagca
     5341 gtccaacccc tgggcgctgc gcgccatctc ggaacgcctg ctcgaggcgg cgcaacgcag
     5401 cctgtggtcc gaaccagatc ccgaagatct cgaagccctg aaacaggttt atctggagaa
     5461 cgaggggatg ttggaagagg gcagcccatg aacgcgtacc ggcatcctta tccattctcg
     5521 gccatcgtcg gccaggagga tctgaaactc gccctgctcg ttaacgccgt ctctccagag
     5581 gtggggggcg tgctcgtacg cggcgagaag gggacggcca agtctacggc ggtacgggcg
     5641 ctggcggggc tgctgcccgg catacgggtc gtctctggct gtccttattc ctgcgatccg
     5701 gattcccccg acccgggatg cccggcggga ccgcacccgt ccgatgcctc cgccgagacg
     5761 aggccggtgc ggctcgtgga gctgcctgtg ggggcgagca cggaccgtct ggccgggact
     5821 ctggacatcg agaaggcgct ctcggagggg aggaaggcgt tcgagccggg gttgctcgcc
     5881 gcggcgcaca ggggcatcct gtacgtggac gaggtgaacc tgctctccga ccacctcgtg
     5941 gatctgctcc tcgacgtggc cgctatgggc gtgaattatg tcgagcgcga gggcgtgagc
     6001 gtgcggcatc cgtcgcgctt tatcctggtc ggcacgatga accccgagga gggcgagctc
     6061 cgcccgcaac tactggatcg cttcggcgtc accgtcgagg tgtcggggag cccggacccc
     6121 gcagaccggg tcgaggtcgt caggcgcagg ctacgctacg aggccgcccc tgaggagttc
     6181 tccggagaat ggtcgaacgc ggacgggaaa ctcgcggctt ccgtggaatc ggcccgtatg
     6241 cggcttccac aagtggagct ggacgaagac atcctgttca gaatctctgc cctctgcgcg
     6301 gaactcgacg tcgacggcct gcgcggcgat ctcgtaactg cgaagaccgc gcttacgctt
     6361 gcggcctggg acgacaggaa cgaagtggta ctcgacgacg tgaaacgggc ggccctgctg
     6421 gccctctcgc accgccgcag acgcggcccc ttcgagcagc cgggcataga tcccgaggag
     6481 ctggagaatg ctctagccga ggacccggac ccgccagatg gcccggacgg tggtatggcc
     6541 ccgccttcta tgagcgagga tgacggcgag gcttcaagta ccgtgagaga tcccgaaccg
     6601 tcggaggccc ggacgggagc cggagagcgg agccatgcgg catcagagcc attcgacccc
     6661 gtgcgactcg aggtcaccga gaagggcagg ggagggcctc tcggccgcag gagccggatc
     6721 tcgggcgagg ccgggcatcc cgtcgcggat cgggagccta aagagaaccc gagggacgtg
     6781 gcgctggcgg cgacggtgag ggctgcggcg ccgcaccaga gggcgcgggg ccgggaggga
     6841 cccggaatcg aggttcgtac ctgcgatctg cgtcagaacg tcagggaggg gagggagggg
     6901 aatctgatcc tcttcctcgt cgatgcgagc ggctcgatgg cggcccgcaa acgcatgtcc
     6961 gccgtcaagg gcgccgtact ctccctgttg aacgacgcct accagcgccg cgataaggtc
     7021 gccctgatct ccttccgtgg cgagggtgcc aacatcctcc tgcccccaac gtcgagcgta
     7081 gcccttgccg ccccgcgcct gcaagacctc ccgaccggtg gacgcacgcc cctcgcggcc
     7141 ggtatcgaga aggccgccga ggcgctcgag agggaggaac tgcgcgacgg ggagcgccgg
     7201 cccctgctgg tcctgcttac cgacgggaag gcgacggcgg ggtccgatcc acgggtggcc
     7261 gccgcgcgac tgcgctccct cacggcgact tcgttcgttg tggacaccga ggagggctac
     7321 gtcaggctcg ggatggccgg cgagctggca gaggccatgg gggcgcggtg cctgcgactg
     7381 gaagaactgc gggccgaagc gcttgtggag ctcgtcgaga ggagaagggt ggcgtgagcg
     7441 agacgcggga accaagagag cagcggctga gaagaaggtc tagcagggaa cggcctctcc
     7501 ttatcgtcaa cacgggccac ggcaagggca agtccacggc ctctttcggg gtcatgctga
     7561 ggggatgggc gcgcggttac cgtatcgggg tctaccagtt cgtcaagagc ggcaaatgga
     7621 acgtcggcga acagaaggcc gccgaggccc tgggcaacat cgactggttc aagcagggcg
     7681 acggatggac gtggaccgta aaggacctgg agcactccgc cgacctcgcc cgcgaggggt
     7741 gggaggaggt caagcgccgt ctcgcagacg agacctacga catgctgctc ctcgacgagt
     7801 tcacgtatcc tatgaagttc ggctgggttg ataccagcga ggtcgttgag gttctgaaga
     7861 accgtcccgg gttccagcac gtcatcatca cgggccgcga cgcaccgcct gaactggtgg
     7921 agatctccga cctcgtgagc gaggtaaaca agatcaaaca ccccttcgac gagggctacc
     7981 ggggccagaa aggcatcgag tggtagatac cctccgcata gtggtcgcag gtacacactc
     8041 gggggtcggc aagacgacta ttacctcggg acttatggcc gcgctctcct caagtggctt
     8101 tcgggtcgcc cccttcaagg tcggcccgga cttcatagac ccctcgtacc acaccctcgc
     8161 tgcggaccga cccgggcgca acctcgacgc cttcctctcc ggcccggagc tcatcggccc
     8221 gctcttcgcc cacggcgcgc agggtgccga catagccgtg atcgaaggcg tcatgggcct
     8281 cttcgatggc aaaggaggcg gggatctcgc ctccactgcc cacgtcgcca gactgctcga
     8341 agcccccata gtgctcgtcg tcgacgccag agcgatgtcc cgctccgtgg ccgccgtggt
     8401 ccatggctac gcgacattcg acccgaaggt gagagtcgca ggcgtcgtcc tgaaccgagt
     8461 cggctccacc tcccacgagg cgatgctccg cgaagccatc aaacccctag gtatccccgt
     8521 cctcggcgtc ctgcgccgcg acgaccagat acagacgccg gataggcacc tcggcctcgt
     8581 ccccgtcgcc gaacgccgcg aggaggccgc ccgatctctc gatgtccttg gaatgatgat
     8641 ctctcgttct ctaaacctcg atggcatagt tagactcgcc cgttccgcag gatccctaga
     8701 agtcgaggcc tggagtcctg agactcggga cctggatcgg tatgaggtgg cccgtgtggc
     8761 agtcgcgact ggaccttcgt tcagcttcct gtaccgcgag aacatggagc tccttcaggg
     8821 cgcgggcgcg gaggtcgtca cgttcgaccc gactaccgac gaggccctgc ccgctggaac
     8881 agacgctctc tacctcggtg gtgggttccc cgaaacctac gcggaggcgc tgtctatgaa
     8941 tggccggatg cgcgagaacg tccgaagctt cgctgagagc gggcgtccgg tggttgccga
     9001 gtgcggtggt ctactgtacc tggtgcgcga gctggacggg tatcccatgt gcggcgtgct
     9061 cgatgctgag gccacgatga tggacaggct taccctcggt tacagggaag cccgagctct
     9121 gatggactcg ccgctggccg agcatggcgc cgaggttcgc ggacacgagt tccactactc
     9181 ggccgtcgag cctggagccg gtgagaagcc ggcctgggag ctggtcggcc gcggtcagga
     9241 gggctttgtg aacggtggcg tgcacgcgag ctacctgcac acccactggg ccgcgacgcc
     9301 ggaattgccg cgcaggctgg tgcaggaggc gacgcggagc gcgacggggg tcggagtatg
     9361 agcgggcggc tagtcggggt gggggtaggc ccgggcgacc ctgagctgtt gacggtcaag
     9421 ggactgcgcg tactgcggga ggccgatgag gtcttcgtgc ccgtggccga caccggcgag
     9481 gtcgggcggg ccgaggccac ggtgcgcgag tacgtggacg aagggcgaat caaacgcctc
     9541 ctcttcgccc tctcggacga tttggaggcc agggcgcgta actggacgaa cgcggctcag
     9601 gatgtctcgg agcacctgcg gggcggcaag acgtgcgcgt tcgccaccat cggtgatcct
     9661 aacgtctatt cgaccttcac ctacctggcg cgtgccgttc gggagatcga acctgacgtt
     9721 caggtagaga ctgtgccagg gatcacggcc cttcaggacc tggcttcgcg gagtgggacg
     9781 gtacttctcg aaggcgacga gcggctggcg ctgttgcctt tcacggcggg ggaggagccc
     9841 ctcagggagg cgctcgccgg gttcgagacg gtggtctgct acaagggcgg aagtcggctc
     9901 gggggggtgc tccgggtcgc cgaagaggga ggtcggctgg agggcgccgt ctacggggcg
     9961 aggctcggga tcgagggcga agaagtcgtg ccagccctgg agatggtggg acgagaaggg
    10021 acgtatctct ccacggtgat ctttaccggg aggagttccg agcttgggta aggtgtggtt
    10081 tatcggggcg gggccggggg cgccagactt gctgacggtc aggggggcca gactcatcgg
    10141 cgaggccgac gtggtgatct gggccagaag cctcgtccag gagggcgtcc tccaatacgc
    10201 ccgacccggt gccgtcatcg tcgaatcgac gaccatacct ctagagggcg tacgtgagct
    10261 ataccagaga gcagtggatg aggactcgag agtggcgagg gtgcattcgg gggatcccag
    10321 tctgtacggt gccgtgatgg agcagatcga gctttgcgag gagatcggcc tcgattggga
    10381 gatggtccct ggagtctcgt ctctaggcgc agcggctgcg gccatcggac gcgagttgac
    10441 cgtgcccgag atctcccagt ctgtgattct gactcgtcgc gcgagccgca ccccgatgcc
    10501 cgagggcgaa gaaataagga gtttcgcgga gcacgggacg accatggcga tctttctctc
    10561 cgcagcgaaa ccgcgcgtcc tgcagcagga actactggaa ggtggttacg ctcccgagac
    10621 cccttgtgcc gtggtctaca gggcgagctg gcccgacgaa gaggttatag agtgccgcct
    10681 cgaagacctc gccgacaggg tgagggaggc aggatttacc aggcaggcgc tcatactggt
    10741 cggtccggga ctcggggccg gagggacacg ctctcatctc tacagccccg tgttctcgca
    10801 catgttccgc cgggcgaatc ctgcacagaa gggagcagat gccgccacgt aaactccgcg
    10861 agccttccat gcccccaagc atggagaagg tcaagggcca gagactcagg acgggctgga
    10921 ccacagggac ctgtgccgcc gccgccgcca aagccgcggc cagtgcactg gtgagtggcc
    10981 ggcggcaggc tcgcgtcgat gtaaagttgc cggggaaagg ccgggagcga cgcgtcgagt
    11041 tcggggtgga gcgctgtgat ctgggcgctt cgtgggccga ggccgtcgtg atcaaagacg
    11101 ccggcgacga ccccgacgta accgacggcg cccatctcac cgcccacatc tcgtggcggg
    11161 aagagcaggg gttcgaactg gatcggggcg atggggtcgg cctcgtcacc aaaccggggc
    11221 tcgggctccc cgtcggcgga cctgccataa acgccgtgcc gcggcgaatg atctcctatt
    11281 ccgtagagga agtcctcgac ccaaacgagc gcggcgtccg cgtagtcata agtgtccctg
    11341 gcggcgagaa gatggccgag aagacgacca acgcccgcct cgggatcgtc ggcggcatct
    11401 caatcctcgg caccaccggc atcgtgcgtc ctttctcgac ggcggcatgg gccgcgagcg
    11461 tggttcaggc catagacgtc atgggcgcgc agggccacaa caccttcgtc ctctcgaccg
    11521 ggggcctcac cgagaaggcg gcgatgcgcc tcctgcccga cctcgaagag gtgaacttcg
    11581 tggaggtcgg ggacttcacc gggcaggccc tgaagaaggc cgtggagaac agcctcgagc
    11641 gctgcttctt cgtcggtatg gtgggcaaga tctccaagct cgccgccggt gtcatgatga
    11701 cccactggac ccgctcgaaa gtcgacaacg aactgctggc acggataacc ctggaagcgg
    11761 gtggcccgac taacctggtc gaagaggtaa aggaagccaa tacggcccgc cacgcctacg
    11821 aactctggcg atcggcgaac ctcgaaaggg cgtctgacct cctctgtaaa caggctgccg
    11881 agaaccttgc cgagtacact caaggtaaac tagaggttca tgttattctc gtggatttcg
    11941 acactctcga accggcgggg gccagtcccg gcgccctggt cttgacggcg tggggccctg
    12001 tgtgaaccgg ggtcgcataa ccgtcgtcgg tctcgatgga aggccgctcg gcgaggagat
    12061 cgaacgtctg ctcgcggatg cggcgctcgt cgccggtggt gggcggcacc tggaaatgtt
    12121 cgaagttgac cgcgaccgtg ccgtggtcct gaagggtgac ctctctgagg cgttcgtgcg
    12181 gatcgaagac acagaaggtc ctgtggtagt gctcgcgtcc ggggaccctg gtttcttcgg
    12241 catcgtgcgt ctccttggcg aacgattcgg gagggagacc ttgcgtgtct tgcctggcct
    12301 ctcctcagta gcactggtct tcgcccgtgc cggtctctcg tggaacgatg ccgtcacggt
    12361 cagcgcccac ggccgtgatc cccatcgcgc cgtcaacgtc tgccgcactc accccaaggt
    12421 cgccgtcctc acctcccccg acttcggacc ggccgaactt gcggaagcgc tcggcgggct
    12481 ggaccgaacc tttgttgttg ccgagaagct gggtgaggcg gacgaacggg tatttcaggg
    12541 agacgccggc acggtcgccg gcaaggagtg gaaagagcct aacgtggtac tcgtgttcga
    12601 cgaagtgagg atgccgggag ccagaggttg gatctcctct agtccccaga gtccgtcgcg
    12661 gtgggcgctc tccgaagagg agttcgagca ccggtctggc atgatcacga agccagcgac
    12721 ccgcgctttc gtactctcgc gtctcggtcc gggaccgggg gacctgatct gggacatcgg
    12781 ggccggcagc ggatcggtag ccatcgagtg cgcacgtctt ggcgctgccg ttatcgccat
    12841 cgaacgcgat cccgagagct gcgcccttat aagccgcaac gccgaacatc acggtgtccg
    12901 gttgcagatc atggaagccc cggccccggg ggtcatgctt gacctgcctg aacccgacgc
    12961 ggtcttcata ggcgggaccg gcggcgcctt cgaggagatc gtcaagctct ccgccgtccg
    13021 ggcgcgccgg gcggtcgtat tggccctcgt caccctcgaa cgggtcgttc ctgccgcaca
    13081 gctcctcgaa gatcgcggcc tcgaggtaga gtccaccttc ctgcaggcgt cccggatgaa
    13141 gggcgtcggt aatctgcacc gtctcgctgc cgaaagcgcc gtcttcgtca tctcggggag
    13201 caggcagtga tcgggctcct ggcagtcacc gcgaacggcc gccgcaacgc cgcacacctc
    13261 gccggcgcat ggcctgatgc gcgcctctac gagggcaagg cgaaagaagt tctccaccgg
    13321 gcctggaacg agtgcgacgg tatcgtgctc ttcctcgcca cgggcgccgc cgtccggctc
    13381 gtcgcgccgc tgctcgagag caagcatcga gatcccggcg tggtcaccgt cgacgatgcc
    13441 ggcaacttcg ccgtagccct ctgcggtggg cacgaaggcg gggctaacgt cctcgcagta
    13501 agggtcgcgg agaccctggg agggacgccg gtgatcacca ccgcaagcga ctccgtcggc
    13561 gtatccgccc tcgactctct ggtagagaaa attggtctcc ggttggaggg aggctcggat
    13621 ctggcttccg tcggagccgc gctcgtctcc ggcgagaggg tcagcctcgt cacggacagg
    13681 cgctttccgc tcggaccgct acccgagaac gtcgtcagga cggagcaatg ggaggcgccg
    13741 atgatcctca tctccgaccg gctcgtggag acgctccgtc ccgctgtcgt ctaccgtcca
    13801 ccctcgctcg tcgccggggt cggttgcagc aggggagcgg gtgcggaaga gatactcgac
    13861 cttctccgcc gctccctggc gcaagccgga ttggctgaga agagcgtcgc ggccctcgcc
    13921 agtatcgacg tgaagcgcga cgaggcggga ttgctcgaag ccgcggagaa gctggacacc
    13981 ccgatccact tccatccggc cgaagcactt tctatagtca aggcgccgaa cccgtctgag
    14041 gtagttcgag aggcggtagg gacgccgagc gtcgccgagg ctgcggtagt gtcctgcggc
    14101 gcggagttgg tactccagaa gcggaaatcc gcgaacgcca cggtcgctat cgggcggcta
    14161 ccggtacggg ggaggcttgc gctggttagc ctgggacctg gcgaagacac cctcattcca
    14221 ccactggccc gagaggcgct cgcagactcg gagatcgtaa tcgggttgga tcagtacgtc
    14281 gaccgcatta ggcatctact gcggccaggg acacgggtgc tcacgccacc gctcggcaat
    14341 gaggtggagc gggcggagct cgcgctcggc gaagcccgcg cgggcggcaa cgtggctctg
    14401 gtatcgagcg gtgacgtggg tatctatgcg atggcctctc cggcgctcga gctggccggc
    14461 gaggacgtcg acgtggtggt agtgcctggg gtaacggcgg cgcaggcggc ggcttcgttg
    14521 ctcgggtcgc cgctcgggca cgaccattgt tcggtcagcc tctccgactt gttgacgccg
    14581 tggggtgtga tccaggaccg ggtcagagcc gctgccgagg gagacttcgt ggtctcgctc
    14641 tacaacccgc gctccaaggg ccgcgactgg cagctcggaa aggcgaagga gatcctgctc
    14701 gagcaccgcc ctcccgatac gcctgtcggc atagtgaaga acgcctaccg cccgacgcag
    14761 caggtcatca tcacagatct cgcatccctg ctcccggaag acgtggacat gctgaccatc
    14821 gtcctcgtcg gaagctccca gaccagggtc gtggcagggc gcatggtaac cccgagggga
    14881 tacctgtggt gaaggcggtc ttcgccgtga cggacgtttg cgccggctgt ggcgcctgcc
    14941 tgaagacctg cccggagaag gcgatccgcc ccgctcccag ggaccacgga gcgcccctcc
    15001 tcgtcctcga agaccgctgt tcgggttgcg ccgagtgcgc cgaggtctgc ccggtctcgg
    15061 catgtgtcga gatactcctg gaggccgaat gaggcgtatc catcccatag agacagagag
    15121 ctaccgtatc ctgcgcgaga tcgtcgacct ctcccacctt ggacccctct ctcgcgccgt
    15181 cgcggagcga gtcatccacg cctcggcgga cctggactac gcggagaacc tcgtcctcga
    15241 cgaggcggcc ctggaaggcg ggatctcggc gctgcggcgt ggcgaaccgg tcgttaccga
    15301 cgtggggatg gtggcggccg ggatcactgg ccgccagaca ctgtgcttcg tctccgaccc
    15361 ccgcgcccag gcgttgtccg agcagcccgg cataacgcgt tctgcggccg gcttcagggt
    15421 cgctgcggag gaggtcggta atggagccgt ctgggtcgtc gggaacgcgc ctacggcgct
    15481 cttcgaactg ctcggactcg acgtagctcc ctcgctcgtg atcggtcttc ccgtcggctt
    15541 cgtcggggcg gccgagtcga agagtgcgct cctgcacagc ggcctgcccg ccgtcgcgaa
    15601 ccggggaccg aaaggcggtt ctgcggtcgc ggcagcggcc ctgaacgctc tcatctactg
    15661 tgaggaggga tgacatgagg cgaatggtga gcaccgagaa cggaacctac gaaaacggag
    15721 atgccccggc gctgctgatc ataggccacg gcagccgtga tcctcggggg gcaaaagagt
    15781 ttcacgagct ggtcgacctc gtgcgccgaa gaaacccgtc cctgaccgtc gagggaggct
    15841 ttatcgagct ctcccggcca cccatctcgg agtgcgtgga ccagctcgcc gagagagggg
    15901 cgaggaacgt cgccgccgtt cctttgatgc tcctggccgc gggccacgcc aaggacgata
    15961 tccccgcaac cctggtgcgc gagaagatgg gccaccccga gatgagcttc agctacggtc
    16021 gcgccctcgg cataaggccc gagctcctcg aactgatgga agagaggatc tccgccgtcg
    16081 tccacgaaga ggagaaggag gagacggcag tcctggtcgt aggtcgcggt tcctccgacc
    16141 ccgatgccaa ctccgatctc tccaagattg cgcgcctgtt ctacgaggga cgcccttacc
    16201 ccgtagtcga gagcgcttac gtcagcatga ccccacccga tgtcgcgaac ggcctggatc
    16261 gctgcctcaa actcggcgcg aagcgcgtcg tggtcttctc ctacttcctc ttcaccggcg
    16321 tcctcgagga gcgtatccgt ggtcagagcc gagcatttgc tgaggggaat ccaggcgtcg
    16381 aggcgctcta tgcagggtac ttcgggcctg atccgagtct ggctgacctt gtggtcgagc
    16441 gttatacaga ggccctgaga ggagacatcc gcatgaactg cgacgtgtgc gtccatcgcg
    16501 tcgccttgcc cggcttcgag gagaaggtcg gcgccccggc aacgccccac taccaccccg
    16561 atgagccagg gcaccagcag cacggccacc atcactagcc gtggccctgg aagaccgctt
    16621 gctggagctc ggcgagggaa tacctccgcc cgacgaggcc gccgccgaag aagcgcgtag
    16681 gcggcacatc gggctcacca aaccgccagg cagcctggga cggctggagg agctcggtgc
    16741 gcggctcgct gggatggcag gtgagcgccc gcccgccccc gagagccctg cagtggtcgt
    16801 ctgcgccggc gaccacggcg tcctcgaacg cggcatctcg ccgtggtccc aggaggttac
    16861 cgccgcaatg gtggggaact tttgcacagg aggggcggcg gtgaacggcg tcgcgaagac
    16921 ggtgggggcg agggtgagcg ttctcgacgt cggcgtcgcc ggggagttgg agaggcatcc
    16981 tctgctacgc gccgcgaagg tgcgaggtgg gacaaatgac ctgagtcgcg ggcctgcgat
    17041 gactcgcgag gaggccgcgc gggccgtgat ggcgggggcg ggagtcgtgg aggaactcgt
    17101 cgagagcggg ggtgtagatc tcctggtaac gggcgacatg ggcatcggga acaccacacc
    17161 agcagcctgt ctcatctccg cctttacggg aagcctgccc caggagacca cgggtcgcgg
    17221 cacgggcatc gacgaccaga ccctgaagct caaggtcaac gtagtggaag aggctttgga
    17281 gctgcacgag ccagaccccg aagacccact cggtgtcctt gcgcaggtcg gtggtctgga
    17341 gcacgctgct gtcgtcggtg tgatcctggc cggcgccgtg taccgactcc cggtcatcct
    17401 cgacggtgta gtctcgaact ccgccgcact cgtagcccgc gccctcgctc cgcactcggc
    17461 gggatacatt attgcgggcc accgctccgc cgagccggga gcaagcatct ccctccgatc
    17521 ccttaagctg cctccgctcc tcgacctgga catgcgactc ggcgaaggga caggaggcct
    17581 gctcgccgtg ccgctcgtgc aggcggcggc ccggacgctc gccgagatgg caacgctcga
    17641 ggagctgggc attggttgag ccgctgaacc agggagccat cgagtccttg aacagcccgc
    17701 tcgcccaggt agtcgaggta cacggggctc tggactcgac ccaggagcgg gcccgcgagc
    17761 tggccagagc cggatcaccg aacgggacgc tcgtcgtcgc ggaggtccag accgggggac
    17821 gtgggaggct cggacgccat tggggatcgc cgataggtgg actgtggatg tcactcatgc
    17881 tgcgtccccg gttcgctgcg agtctcgcat ctcgcataac ccagacggct gcggtcggcg
    17941 tagcgaaagc ggtctgggag gtcggggtcg aggtccgtat caagtggccc aacgacctgc
    18001 tcgccggcgg gaagaaggtc tgcggcatcc ttgccgaatc gagcgcgggg aacgctggcg
    18061 aaacggtaga ggagaggcgc ctcgactacg tcatcctcgg ggtggggatg aacgccaacc
    18121 tcgacccggc cgagctcggg gttcagaatc gagaggttac cacgatccgt gccgagctcg
    18181 gccgtgacgt agacctgctg gagctgctgc gcgccctgct ctccagcctc gacgctgagc
    18241 tgggtaggat cgaggacttc gcggccgtgc tcgaggactg gcgcaacttg aactgtacct
    18301 tgggagagaa ggtacgcgtc cggcgcttcg gcgaaaccgt cgaaggcagg gccgtggacc
    18361 tgagtcggga gggtgcccta ttgctctcta cgagagaggg gactgtggag ttattcgagg
    18421 gcgagatcga acacctcagg caaggagtcg atatcctgta gcctgagacc tgttgccttt
    18481 cgaagcggaa gatcagtcct ctcgcacacg cttcagccgg taggcatctc ctcgtcttct
    18541 tcatcctctt ccagaaccgc aaccgaggtg gccctgaaga ccaggatctt acccctctcg
    18601 gggaagtcta cgatctccac gtccccggtt acggctatct ggtccccgac taactcgtcg
    18661 gcatcctgta cggccgacat atctatctcc gtctccagga catagcccgt atgctcctct
    18721 ccggcttccg tactcgcgcg cttgagctgt ccttcgaaat aggccatcgt agatcccctt
    18781 tcagagtgtc aggtgaggac atagtaccct tattcgcgaa cgaggtccgg ggcatcgtct
    18841 tctataccag gcacggcgag gccctgcgac caccgagagc gcttcgaaag ggttccggcc
    18901 ttccgcgcgt cacgctcaga cgcgattaga gttgccgcga gatttctcga agtgggcgct
    18961 tcgggcagga ccggttgcta ttctatcgct tgtaggattt aatgcgctgt gcaggagcct
    19021 cgatcctgga tcgggcagcg ttgagaggaa gacaagaggg atgagcgcaa cgttgatgta
    19081 cgtcggcatc ggggccgtac tcgctacatg cgtcgcgatc ctcactatcg ctgtcctatt
    19141 cttgcggacc gcgcgccgct acgtcgacct gaccgagagg cgactggagc tcctgcacga
    19201 aagggaagcg ttactgctcg cgctcgtgca gcgacaaggc cacgctccag aggagagcca
    19261 ggagcgggaa gtggagcggc gcgagaaggt ccccgagatg gtcggatata ctcgtggacg
    19321 gcggcaggac gaagcatccc ctggcagcag gcggcctgaa gggctggacc ggcacgtccg
    19381 gggaacgccg tataggggca gctcctctga agtagaagat gaagaggaga ggacaccgaa
    19441 ggaggacccg gagccggcgc ggcccaaaga aggttcgccg cggcttggag tccaggtgcc
    19501 gcaccctgac gacgatgtgc cgggacgcgg ctggaacaag gctcctgcca ggttcttcca
    19561 gaagtgctac gacagatacc tggagcacta cgagggctac gtacggctcg ccgagcggct
    19621 ccaccagatg cgcgacgggg tcgatgcggc accaggctcc ctcgtgaagc gggagtggga
    19681 gaacaagctc cgccgcgctt acgatgccat agatcggact acccagaggc tggacatgct
    19741 cgaggagcac taccccgagt tggcgaccga caacgatcgc atctcgtccc gcatcggtac
    19801 cgcacgcctc catgccggcc tcacagaacg cttcggccgc ccacaataag cacggagttc
    19861 gaaacccccg agtctcgccg ggcccggagg ggcctacgcc cctgactcca gagacgtcga
    19921 tgccgtcagt gccggtgcca ccgaataccg tgtcgtcgcc gtttccggcg aacatttcat
    19981 cggtgccgct gccaccacgg agctcgtccc tccacagctg cagatgaagg gcacgttgcc
    20041 gcccgcagaa cgaaacctca acctaatcgt tcccgatctt cgcgtacccg gttctggtgc
    20101 gcagtcgcag gcggtcgccc gaagcattaa ccgtggggat cgcggcctcg tccattgcat
    20161 cggagtgtcg gatagaagac gagggcgagc ggaatgggta gccagaagtt gaagaggcgg
    20221 taaccgagga tcggtatcgc ggcttcgggg ccaaccccga gcagggcgag ggtggcgagc
    20281 atggtaacct cgaagatacc tatgcccccc ggtgtgagcg gtatggccgc gatggtggtg
    20341 gccaccccgt aggccacgag gagaacgaag ggatcggccg ccacgccaag ggcgtcgaac
    20401 atgaggatca ggcaaagggc gtcgaaggcc cagtagccca gcgccagcgc cgcgagcttg
    20461 ggcacctctg agggccgtgc ggccagctgc cggcgcgccg cctgaagctc ttctccgagt
    20521 cgtgagatga gccgggcgga ccacgtcctt gctcttcgcc gccacccttc gccgc
//
LOCUS       DATMVF010000128        12949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_655443, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000128 DATMVF010000000
VERSION     DATMVF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12949
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655443"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..491
                     /locus_tag="VN178_06565"
     CDS             180..491
                     /locus_tag="VN178_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80672.1"
                     /translation="MPGYNRAELSVEITILPSVRGAKAPQKQKEAGMRAANASGLSIH
                     EAGPDTTVLAGRRGEALDAAREVVEAALDAGVGEVKIKVEAQGESAKFGSAGGRRAPA
                     R"
     gene            530..1051
                     /gene="uraD"
                     /locus_tag="VN178_06570"
     CDS             530..1051
                     /gene="uraD"
                     /locus_tag="VN178_06570"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HWS80673.1"
                     /translation="MPEKITVEEVNRLSRKGFVERFEGLYEHSPWVAEEAYRARPFSG
                     LDGLHSAMARAVEAAPEGRKMALILAHPDLAGRAAMAGELTLASKREQASAGLDALSP
                     GEYREFHRVNDAYKEKFGFPLIFAVREHTKETILAGAERRLEHSRPEEIETALAEISK
                     IARLRLEDLVEQD"
     gene            complement(1114..1371)
                     /locus_tag="VN178_06575"
     CDS             complement(1114..1371)
                     /locus_tag="VN178_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80674.1"
                     /translation="MEREPLSSDADALWRRLWEIWQDNGEEDVVLDSTELAALEEEIP
                     GLENRMKTALAYLQRARYIQYRSGVGEDGLEPILYDVYEPR"
     gene            complement(1376..1900)
                     /locus_tag="VN178_06580"
     CDS             complement(1376..1900)
                     /locus_tag="VN178_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWS80675.1"
                     /translation="MSESVHKHRESAPETVRVAILTISDTRTRETDTGGDTAEQILRE
                     ADQEIVARQIVRDEVNGIRTSLIDLLANPEVDAVVTTGGTGISGRDTTYEVVDRMIEK
                     KLDGFGEIFRMLSYEEIGAAAIMSRAIAGAVGTKFVASLPGSRNAVRLAVEKLLVPEL
                     AHIVFELRKHEESG"
     gene            complement(1897..2535)
                     /locus_tag="VN178_06585"
     CDS             complement(1897..2535)
                     /locus_tag="VN178_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80676.1"
                     /translation="MILEMLTVSPFQENCYIIGDEESGVGAVVDPGDEAARIALAVEQ
                     TGLDVASIIVTHAHIDHVGAVAALVDEYACPVLMHAEAEPMLRQLPTQAIMMGLKFGK
                     VPAVDRYIGDEEVLEVGDLRLRSLYTPGHAPGHLAFYVEDEGLVLSGDALFAGSVGRV
                     DLPGGSMEVLMRSIEERLLTLPDETRVFPGHGPQTEIGNERASNPFLQGGLF"
     gene            complement(2532..3980)
                     /locus_tag="VN178_06590"
     CDS             complement(2532..3980)
                     /locus_tag="VN178_06590"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWS80677.1"
                     /translation="MVTIGATSRASEEFAPELLTVALYKGESPQDLGEDAARVVSDGD
                     FGGKAGETALLYGQDGPAPRLLLVGLGERDLFTLERLRRAAATAARRARALKLQSAAF
                     SLPALPDTREIARTVAEGARLGLYRFDRHKSAAEDHDLETFWLVADEGDLDGAAEGAE
                     LGSRVAEGTVLARDLANEPSNVATPEYLAQRTRAIAEKYGMELDVLDRAGIEEEGLTG
                     LATVGRSASNEPYFIVLEHRGGGDSSPIVIIGKAVTFDSGGISIKPSAGMEDMKFDMS
                     GGAAVLGAMEAAGALDLPLNVVAIVPATENLPGGDAFKPGDVLRMHSGKTVEIVTTDA
                     EGRLILADALSYARRYEPSAVVDCATLTGACVVALGDHASGLMGNDEDLIAELQAAGE
                     STGERAWTLPLLEEYTEQIRGDTADIKNSGGRKGGALTAGAFLKEFADYPWAHLDVAG
                     TAYGKKGNAYTTKGATGVPARLLVEFLLGRTR"
     gene            4125..4898
                     /locus_tag="VN178_06595"
     CDS             4125..4898
                     /locus_tag="VN178_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWS80678.1"
                     /translation="MNLRPYLEFGVQTAYEAGKLTLGYFGTEAARPEFKADDTPVTVA
                     DREAETLIRERIGARYPRHAVLGEEYGEDEGSDADYRWVVDPIDGTKSFVRGVPLYGV
                     LIGLEIEGVCQVGAAYFPAIDEMVCAATGEGCYRNGRRTSVSTTRSLAQGMVSFTDAD
                     SFEEHGRSAAWQRIRSSAGGTRGWSDAYGHALVATGGVDLMLDPIINPWDCGPFPPIL
                     REAGGYFGDWSGNESIYAGEAMSTTRSLLPEILSLLGSR"
     gene            complement(5085..6416)
                     /locus_tag="VN178_06600"
     CDS             complement(5085..6416)
                     /locus_tag="VN178_06600"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWS80679.1"
                     /translation="METADRQSVLRQAEENNVQFMRLWFTDVLGTLKSFAIPVGELED
                     ALDGGMGFDGSSITGYQDIEESDMIAMPDPSTFQVIPWSPPDAPTARMICDVRTPDGD
                     PYVGDPRHVLRRALERANEMGFDKFYCGPELEYFYFRDSKGTEPLDYGGYFDLTTLDA
                     ATALRRETVQSLQQLGIKVEYSHHEVGISQHEIDLRYDDAMAMADKVMTYKTVVKEVA
                     TRHGVYATFMPKPLRDQNGSGMHTHQSLFSGGKNAFFDADNEYYLSSEAQSFIAGQLR
                     HAREISVIFAQHVNSYKRLVPGYEAPVYIAWSRRNRSALVRVPLYHPGQEKATRMELR
                     CPDPAANIYLCFAALLHAGLEGIEKGYELPEPMERNLYHLTAEEREKMGIESLPADLG
                     EAIKEAEQSELLHKALGEHTYTRLLQLKREEFEDYRVQVTPYELERYLPVL"
     gene            6700..7512
                     /locus_tag="VN178_06605"
     CDS             6700..7512
                     /locus_tag="VN178_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80680.1"
                     /translation="MSDRPQEGREERRRTGPLGDEDDETRRVPTENDSEATRQVRSDG
                     DASYETRRVAREPSQEENETRVIRVPDHPDTTRDATPYPRGYFEAAEEREDRLRDMYG
                     GVDWLASFLGFVFAIVLGALFSAVAGLVLIPFGITPDLSGGQIGASVITGLALLGVLV
                     FLTFFFGGYVAGRLARFDGGRNGAMVVVWMFIVFVILALAAAIFSGFLPAGIAEVLAN
                     LLDRILSTADNLAGAGVVGLVTLAAALLLAFLGGSLGGRMGSRYHAEIDRAT"
     gene            7526..7657
                     /locus_tag="VN178_06610"
     CDS             7526..7657
                     /locus_tag="VN178_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80681.1"
                     /translation="MKKAIAIKLIKSKTARRVAVKAMMNPKVRRIVVDQARRRVLGR"
     gene            complement(7654..8781)
                     /locus_tag="VN178_06615"
     CDS             complement(7654..8781)
                     /locus_tag="VN178_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HWS80682.1"
                     /translation="MNTRDHTSLVPENAFEQGTKEGTLGAEEELWLADPGTFKLADGA
                     QKILAHESATLFSGELIDCEIESNTGVHEQPAGVLADLVSRRRILLGHAGRLGRVLGT
                     SGTHPIGDWREQEIIDKPHYQRLKRKLGWLIRRNNTFSVHVHYAVQGRGKAVYLFNRL
                     REYVPHMLALSVNSPFWQGEYTDMHSARVLMFSRAYHRAGLPEPFGAWEEYTDYVDYL
                     TRAGLIDALGEIWWDVRLHPGLGTVEIRAFDAQTDVARNEALVALAAALCDLLALEYD
                     ANELMPIIPNREIEENKWSAQRYGLEGIFIDHETHEAVDTRVAIETLVTRLSGATYRD
                     LSGLERILDGPTESVRQLEVWRETGSTLEVAKDLARRTSAP"
     gene            8956..9837
                     /locus_tag="VN178_06620"
     CDS             8956..9837
                     /locus_tag="VN178_06620"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWS80683.1"
                     /translation="MEHRSTEYVPESLVAACAATAMMLVYSAPTPGTGSRYLDGRVAH
                     ESKALSVLSVRDALAFSGRRGVGETVLDATVSSLSLAGYADPRIAGYLAPLARGALRE
                     EPSARVLAGLGHDEIAPFARALEVAGGEEALAGALSYALGAGKDATLRDAMRFAAGRH
                     ALAREYARDFEVTRELARPALLTSLSRADSARGALVQAYLEVLSEVPDLDVAARAGRQ
                     EAEEVSRMAHGVLKAGGVHSRRGLEGIANLGGLLRADPRLSPTTTEYPVIAAAFLVAL
                     EYGPDALSHRLRPATGW"
     gene            10012..10767
                     /locus_tag="VN178_06625"
     CDS             10012..10767
                     /locus_tag="VN178_06625"
                     /inference="COORDINATES: protein motif:HMM:NF002671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HWS80684.1"
                     /translation="MRALVIVGHGSHLNEDSSLPVYEHAGRIRETGEFDEVVECFWKE
                     EPSMRHVLDTIESDDVYVVPAFISEGYFTQQVIPRELGLEGPLSRKGEKTVRYAGPLG
                     TFEEMPDVILERTEDLMRGKDVSGRTALVLLGHGTDLNKNSGGVIYLNAGRIRERGIY
                     DLVEVGFLDQDPEIGDVVDRIEAENVILIPLFIAEGWHTRETIPEDLGLTGDVTLRPG
                     KTIFYGAPVGTHPSMANLIVARARGTMTGQPVA"
     gene            10760..11575
                     /locus_tag="VN178_06630"
     CDS             10760..11575
                     /locus_tag="VN178_06630"
                     /inference="COORDINATES: protein motif:HMM:NF037718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HWS80685.1"
                     /translation="MPDAHTGRPENAAASSREAFAAWVDDAPQGRVFGQVIVRPAPPL
                     GYSLRHVDDEREDGLRVTEDPHAAREIARLTERGEYRPLKSSPNLRRGWELRVADDRA
                     LATAMNYLYPAGVVHWHLHREGRLDLTSFRENAARQSGIYKRVQRLSDQGVIDAATAC
                     CEDSVCLKKTLWDVDEGTPLEVDRGEGEIPCPEPCSIFVSFARRVRLFEREKDLDPTD
                     LSPSETEDLAALVEAAAAGGVDLAREAEFERPLNERRMRYRRLTLVPKLRRNG"
     gene            11694..12359
                     /locus_tag="VN178_06635"
     CDS             11694..12359
                     /locus_tag="VN178_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWS80686.1"
                     /translation="MARSKRKAAKPGAKAGARVAPVAGRTAGKAAWKLGKAEAKLIRR
                     AMSAQESKKSRYFKYAIFTLIGLAIGAAIARSKKGETSEGSSSYTGTTGPHAPDPGSP
                     AGERGQTWGSGTPVGTAGGANRTGAERDYSDPSSGPLIGEQHSDVEGVGEQQEELEQR
                     IRTQIGEDPRTAEMPRVNVEVNDGIAELRGTAPSEDAKQAAGEIAQGVEGVREVRNLI
                     TVS"
     gene            complement(12500..>12949)
                     /gene="manB"
                     /locus_tag="VN178_06640"
     CDS             complement(12500..>12949)
                     /gene="manB"
                     /locus_tag="VN178_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564118.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWS80687.1"
                     /translation="TKAGHSIIKPQMRKNNAAFGGEHSGHFYFRDNYFADSGIIAMLT
                     IAELVARQEEPVSRLLSPIDPYVRSGEINSEVEDQEETLQKVEDHFSAHDDAKVDYLD
                     GLTVDLGEWWFNLRPSNTEPLLRLNVEAEDKETMERKRDELLSLIRE"
BASE COUNT         2254 a         4149 c         4234 g         2312 t
ORIGIN      
        1 gcccaacatc tccacgcccc ccgcgatcgt cgccgccctg cgcgacgcca cgggaagtga
       61 gctaccccgg atgcccgtcc gtccggaaca gatcgtaggc atggccgaag ggccgccccg
      121 tcgcacgcag ccgtggggcg cgcggccctc ggcaccatga gaggaaggag ggagtactga
      181 tgccgggata caacagggcg gagctcagcg tggagataac gatcctaccc tcagtgcgtg
      241 gcgcgaaagc cccgcagaag cagaaggagg cagggatgcg ggccgcgaac gcctcgggcc
      301 tctcgatcca cgaggccggt cccgacacca ccgtgctcgc cggaaggcgg ggcgaggcgc
      361 tcgacgccgc gagggaggtg gtggaagccg ccctcgacgc cggagtcggc gaggtaaaga
      421 tcaaggtaga agcgcagggc gagtccgcga agttcggcag cgccggcggc agacgcgctc
      481 ctgcccggta gagaaggtaa cgggaggcgt tccagcgaac aggaggagga tgccggagaa
      541 gatcacggtc gaagaagtca atcgcctgag tcgaaaaggg ttcgtagagc gcttcgaagg
      601 actctacgaa cactcaccct gggtcgccga agaggcgtac cgcgcgagac cgttcagtgg
      661 gctcgacggg ctgcacagcg cgatggcgag ggcggtagag gcggcccccg aagggcgcaa
      721 gatggccctc atcctggcgc accccgacct ggcgggaagg gcggccatgg ccggtgagct
      781 cactctcgcg tcgaaaagag agcaggcatc ggcgggcctg gacgcgctga gtccggggga
      841 gtaccgggag tttcatcggg tgaacgacgc ctacaaggag aagttcggct tccccctgat
      901 cttcgccgtc agggaacaca ccaaggagac gatcctcgcc ggcgccgaga ggcgcctgga
      961 gcactcacgt cccgaagaga tcgaaaccgc cctggccgag atatccaaga tagcccgtct
     1021 gcgtctggag gacctggtcg aacaggactg atcacagcga gccccaggtc atactcctca
     1081 cgctcctcga ggctccacca ggttctcacg ggctcagcgc ggctcgtaga cgtcgtagag
     1141 gatgggctcc aggccgtcct cgccgacccc ggagcggtac tggatgtagc gggcgcgctg
     1201 gaggtaggcg agggccgtct tcatccggtt ttcgagacct gggatctcct cttcgagggc
     1261 ggccagctcg gtggaatcga ggaccacatc ctcctcgccg ttgtcctgcc agatctccca
     1321 cagcctgcgc cagagcgcat ccgcatcgga agataggggt tctctctcca tcgctctacc
     1381 cgctttcctc gtgcttgcga agctcgaaca cgatgtgggc cagctcgggg accaggagct
     1441 tctccaccgc gagcctgacc gcgttgcgcg atcccggcag gctcgctacg aacttcgttc
     1501 ctacggctcc agcgatcgcg cggctcatga tcgccgcggc cccgatctcc tcgtaggaca
     1561 gcatcctgaa gatctcaccg aaaccgtcga gcttcttctc gatcatgcgg tcgacgacct
     1621 cgtaggtcgt gtcgcggccc gagatacccg tcccgcccgt cgtaacaaca gcgtccactt
     1681 ctggattcgc aagcaggtct atgagactcg tcctgatgcc gttcacctcg tccctgacga
     1741 tctggcgcgc cacgatctcc tgatccgctt cgcgtaggat ctgttccgcg gtatcgcccc
     1801 ccgtgtcggt ctcgcgggtc ctcgtgtcgc tgatggtcaa aatcgcgacc ctgaccgtct
     1861 ccggggccga ttcccggtgc ttgtggacgc tctcgctcaa aacagccctc cctgcaagaa
     1921 tgggttggat gcccgctcgt tcccgatctc ggtctgtggt ccgtgcccgg gaaagaccct
     1981 cgtctcgtca gggagcgtca gcaaccgctc ttcgatgctg cgcatgagga cctccatgct
     2041 cccgccaggc aggtctaccc ttcccacgct cccggcgaag agggcatcgc cagacaggac
     2101 gagaccttcg tcctcgacgt agaaagccag gtgccctggc gcgtggccgg gtgtgtagag
     2161 cgaccgcagc ctgagatccc cgacctcgag cacttcctca tcgccgatgt aacggtccac
     2221 agctgggacc ttgccgaact tcaggcccat cataatcgcc tgggtcggaa gctgcctcaa
     2281 cataggttct gcctcggcgt gcatcaggac cgggcaggcg tactcgtcga cgagggcggc
     2341 gacggccccg acgtggtcga tgtgggcgtg cgtaacgatt atggacgcga catccagacc
     2401 cgtctgctcg acggcgagcg cgatgcgggc cgcctcgtcg ccgggatcca cgaccgcgcc
     2461 taccccggat tcctcatccc cgatgatgta gcagttctcc tggaaagggc tgacggtgag
     2521 catctccagt atcatcgcgt cctcccgagt aggaactcca cgagcaagcg ggcggggacg
     2581 cccgtcgcgc ccttcgtggt gtaggcgtta ccctttttgc cgtaggccgt tcccgcaaca
     2641 tccaggtgcg cccacgggta atcggcgaac tctttcagga atgcaccggc ggtcaaggct
     2701 cctcctttgc ggccgccgga gttctttatg tccgcggtgt cgccccttat ctgctcggtg
     2761 tactcctcca ggagcggcag cgtccaggcc ctctccccgg tcgactcacc cgcggcctga
     2821 agctctgcga taaggtcctc atcgttcccc atgaggccgg aggcgtggtc cccgagcgcc
     2881 acgacgcagg cgccggtcag ggtggcgcag tccacgacgg cggaaggctc gtagcgcctg
     2941 gcgtaagaga gggcgtcagc gaggatcaaa cgcccctccg cgtcggtcgt aactatctcc
     3001 acggtcttcc ccgagtgcat ccgcagtacg tcgccgggct tgaaagcgtc cccaccgggc
     3061 aggttctcgg tcgctggcac gatcgcgacg acgttgagcg gtaggtcgag ggctcctgct
     3121 gcctccatcg ccccgaggac ggccgcgcca ccgctcatgt cgaacttcat gtcctccatt
     3181 cctgccgacg gcttgatgga gataccaccc gagtcgaagg ttacggcctt acctatgatc
     3241 acgatgggtg acgagtctcc acccccgcgg tgctcgagca cgatgaagta aggctcgttg
     3301 gaggccgagc ggcccacggt agccaggccg gtgagcccct cttcttcgat gccggcccgg
     3361 tcgagaacgt cgagctccat accgtacttt tcggcgatag cgcgggtcct ctgtgcgaga
     3421 tactccggag tagccacgtt ggacggctcg ttggccaggt cgcgggccag cacggtcccc
     3481 tccgcgaccc tggaacccag ttcggcaccc tctgcggccc cgtccaaatc cccttcgtcg
     3541 gcgacgagcc agaacgtttc gagatcatgg tcctcagccg cgctcttgtg cctgtcgaag
     3601 cggtacagtc ccagcctggc cccttcggcg acggtgcgcg cgatctccct tgtatcaggg
     3661 agggccggca gggagaaagc cgcgctctgc aacttgagcg ccctggcgcg tctggcagcg
     3721 gtggccgccg cacggcggag tctctccagt gtgaagagat ccctctcccc gaggcccacg
     3781 agcaagagcc ggggagcagg cccgtcctgg ccgtagagca gggccgtctc gcccgctttg
     3841 ccgccgaaat ctccgtccga cacgactctg gcggcgtctt cgccgaggtc ctgcgggctc
     3901 tcgcctttgt acaatgctac cgtgagcagc tctggcgcga attcctcact tgctcggctc
     3961 gtagcgccga tcgtgaccaa gtccggacca cccttctacg cgagacgcag gaccgcatag
     4021 attatagggg aaaggtagag gccgtctaat ctgcggccgt gtggacacac gtgaactggt
     4081 ctggttcggc atctcggacc ggagaaggat gctatcttgg gggcatgaat ctgcgcccct
     4141 acctcgaatt cggcgtccag actgcttacg aggctggaaa gttaaccctc ggctacttcg
     4201 ggacggaggc ggcgcgtccg gagttcaagg ccgacgatac gcccgtaacg gtggcggacc
     4261 gcgaggccga gaccctcatc cgtgagagga taggggcgcg ctacccgagg cacgcggtcc
     4321 tgggagagga gtacggggag gacgaagggt ccgacgccga ctatcgttgg gtcgtcgatc
     4381 ctatagacgg tacgaagtct ttcgtccgcg gcgttccttt gtacggcgtc ctgatcgggc
     4441 tagagataga aggcgtctgc caggtcgggg ccgcgtattt tccggccatc gacgagatgg
     4501 tctgcgccgc cacaggcgaa ggatgctaca gaaacggccg caggaccagc gtgtcgacca
     4561 caaggagcct ggcgcaaggc atggtttctt tcaccgacgc agacagcttc gaggagcacg
     4621 gtcgctcggc agcctggcag cggattcgct cgtccgcagg aggcacccgc ggctggtccg
     4681 acgcctacgg tcacgccctc gtcgccacgg gcggggtgga cctgatgctc gaccccatta
     4741 tcaacccctg ggactgcgga cctttcccac ccatccttcg cgaggccggc ggatacttcg
     4801 gcgactggtc gggcaacgag agcatctacg cgggagaagc gatgagcacc acgagatcgc
     4861 tcctccccga gattctgagc ctgcttggaa gtcgctaacg cggctacagg cttcaggcat
     4921 caggttacag gcttcaggtc ttctctgagg acaggagcgt ataccaggaa ccgatgtgaa
     4981 tcttgttggt tcggcgatcc gaaaaccgcc gtgaacactg tcccgctcga tcacgagggg
     5041 cccggcatga gccggacccc aacctgtagc ctgaagcctg aaacctacaa aaccggcaag
     5101 tacctttcga gttcgtacgg cgtaacctgc accctgtagt cttcgaactc ctcgcgcttt
     5161 agttgcagga ggcgggtgta ggtgtgctcg cccaaagcct tgtgcaggag ctcggattgt
     5221 tcggcctctt tgatcgcctc cccgaggtct gcgggaaggc tctcgattcc cattttctcg
     5281 cgctcttcgg ccgtcagatg gtagaggttg cgctccatcg gttcgggcag ctcgtagccc
     5341 ttctctatgc cctccaggcc ggcgtgcaag agcgccgcaa agcagaggta gatgttcgcc
     5401 gcggggtcgg ggcagcggag ctccatccgc gtggccttct cctggcccgg gtggtagagc
     5461 gggaccctga ccagcgcgga gcggttgcgg cggctccagg cgatgtagac cggtgcctcg
     5521 tagccgggga ccagccgctt gtaggagttc acgtgctggg cgaagataac gctgatctcc
     5581 cgtgcgtgtc tcagttggcc ggcgatgaac gactgcgctt cgctagagag gtagtactcg
     5641 ttgtcagcgt cgaagaaggc gttcttgcca ccggagaaca ggctctggtg ggtgtgcatc
     5701 cccgagccgt tctggtccct caggggcttg ggcatgaacg tggcgtatac gccgtgacgg
     5761 gtggcgacct ctttgaccac ggttttgtag gtcatcacct tgtcggccat cgccatagcg
     5821 tcgtcgtagc ggaggtcgat ctcgtgctgg gagatgccga cctcgtggtg ggagtactct
     5881 acctttatgc cgagctgctg cagcgactgc accgtctcgc gcctcaaagc cgtcgccgcg
     5941 tcgagcgtcg tcaggtcgaa atagccgccg tagtcgaggg gctccgtccc tttggagtcc
     6001 ctgaagtaga agtattccag ctccggtccg cagtagaact tgtcgaagcc catctcgttc
     6061 gcccgctcca gggcgcgtct caggacgtgc ctggggtcgc cgacgtaggg gtcgccatcc
     6121 ggcgtcctga cgtcgcagat catacgcgcc gtcggcgcat ccggcgggct ccaggggatg
     6181 acctggaacg tcgaggggtc gggcatggct atcatgtccg actcctcgat gtcctggtag
     6241 cccgtgatcg aagacccgtc gaagcccatg cccccgtcga gggcgtcctc aagttccccg
     6301 accgggatcg cgaagctctt cagcgtccct agaacgtcgg tgaaccacag ccgcatgaac
     6361 tgtacgttgt tctcctctgc ctgccgcaat acgctctgcc tgtcagccgt ctccaagctt
     6421 acctccttag gatattcttt ctttaccggg tcccggggcc gggacccgcc agcaacagca
     6481 gccatgatat gaagggcccc cacccgttgc atatggccca gcgtacaagc caggcgccga
     6541 gatcaggccc gattctcggc ctgatgtata aagctgggcc ctggcgccat cctgctgacc
     6601 tgtgcagggt cggcctcgcg ggtacccctg acgcccggca gggtagaatc tacactgtac
     6661 tacagatgcc cgaccgacta tatgacgtaa gggggagaga tgagcgacag gccgcaagag
     6721 ggccgcgagg agcgccgccg tacgggacct cttggagatg aggacgatga gacgcgccgg
     6781 gtacccaccg agaacgactc ggaggccacg cgccaggtcc ggtcggatgg agatgcgtcc
     6841 tacgagaccc ggagggtggc cagggagcct tcccaggagg agaacgagac ccgtgtgatc
     6901 agggtacccg accaccctga cacgaccagg gatgcgacac cgtacccgag gggttacttc
     6961 gaggccgcgg aggagcgtga ggaccggctc agggacatgt acgggggtgt ggattggctg
     7021 gcgagctttc tcgggttcgt cttcgccatc gtcctggggg cgctgttctc ggccgtcgcc
     7081 gggctcgtgc tcatcccctt cggcatcacg cccgaccttt cgggcgggca gataggggcc
     7141 tcggtcataa cgggcctcgc cctgcttggg gtcttggtct tcctgacctt cttcttcggc
     7201 gggtacgtag ccgggcgtct cgcgcgtttc gacggggggc gcaacggggc catggtggtg
     7261 gtctggatgt ttatcgtctt cgtgatcctc gccctggcgg ccgcgatctt cagcggcttc
     7321 ctgcccgccg ggatagccga ggtactcgcc aacctactcg acaggatctt gtccacggcc
     7381 gacaacctgg ccggtgccgg cgtcgtcggc ctcgtaaccc tcgcagccgc cttgctcctt
     7441 gccttccttg gaggatctct gggtgggcgc atgggcagca ggtaccacgc cgagatagac
     7501 agggcgacct gacaggaggt gagtcgtgaa aaaggctatc gcaatcaaac tgatcaagag
     7561 caagacggcg cgtcgggtgg ccgtcaaggc tatgatgaac ccgaaggttc gcaggatcgt
     7621 cgtagaccag gccaggcgca gggtgctcgg gcgctagggc gccgaggtcc tacgcgccag
     7681 gtccttcgcg acctcgagcg tggatcccgt ctctcgccac acctctagct ggcgcacgga
     7741 ttctgtcgga ccatccagga tgcgctcgag acccgagagg tccctgtaag tggccccgga
     7801 gagtcgcgtc accagcgtct cgatggccac gcgggtatcg acggcctcgt gggtctcgtg
     7861 gtctatgaag atgccttcca ggccgtaacg ctgggcgctc cacttgttct cttctatctc
     7921 ccggttcggg atgatgggca tcagttcgtt tgcgtcgtac tccagggcca gcaagtcgca
     7981 cagggcggcg gcgagggcga cgagggcttc gttgcgggcc acgtccgtct gggcgtcgaa
     8041 ggccctgatc tccacggtgc cgagacccgg gtggagcctc acgtcccacc agatctcccc
     8101 gagggcgtct atgaggccgg cgcgcgtgag gtagtcgacg taatccgtgt actcctccca
     8161 ggcgccgaag ggttcgggca gcccggcgcg atggtaggcg cgcgagaaca taaggacgcg
     8221 cgccgagtgc atgtccgtgt actcgccctg ccagaatgga gagttgaccg agagcgccag
     8281 catgtgcggc acgtactcgc gcaggcggtt gaagaggtaa acggccttcc cgcggccctg
     8341 cacggcgtag tggacgtgca cggagaacgt gttgttgcgc cggatcaacc accccagttt
     8401 ccttttcagg cgctggtaat ggggcttgtc gatgatctcc tgctcccgcc agtcgccgat
     8461 gggatgcgtc ccgctggtgc cgaggaccct gccaagccgt cctgcatgcc cgagcaggat
     8521 gcgcctgcgg gagacgaggt cggccagaac tcctgctggt tgctcatgaa cgccggtatt
     8581 cgactcgatc tcacagtcta tgagctcgcc tgagaacagg gtcgcgctct cgtgggccag
     8641 tatcttctgt gccccatcag cgagcttgaa ggttcccggg tcggcgagcc acagctcctc
     8701 ttccgcgccg agggtgccct ccttcgtgcc ctgctcgaag gcgttctcgg gtacgaggga
     8761 ggtgtggtcg cgggtattca aggccttgct cggcgagagt cggacgctct tatgtggtgt
     8821 ggtgtgtgaa ccatggcgac gagtatatta ctctgtccgc acggagtgtc gagcgcctcg
     8881 ggttagaatg gtgccatcta cagccggacg ggaggtgttt gtgcctgcgg accttgacgg
     8941 ggcgcagtag cctggatgga gcatcggagc acagagtacg tacccgaaag tctagttgcg
     9001 gcctgcgccg cgacggccat gatgctcgtc tacagcgccc ctaccccggg gacggggtcc
     9061 cgctacctcg acgggcgcgt cgcgcacgag tcgaaggccc tctcagtact atcggtcagg
     9121 gatgccctcg ccttctcggg ccgcaggggc gtgggggaga cggtcctaga tgccaccgtc
     9181 tcttcactct ctctggccgg ctacgccgat cctcgaatcg ccgggtacct ggccccgctg
     9241 gcccgcggcg ccctgcgcga agagccatcg gcccgggttc tcgcaggact cggacacgac
     9301 gagatagcgc cgtttgcgcg ggcgcttgag gtggccggcg gcgaagaggc cctcgccggt
     9361 gcgctatctt acgcgctcgg ggcgggtaaa gacgccacgc tccgcgacgc gatgcgcttc
     9421 gcggcggggc gccacgccct ggcccgagag tacgccaggg acttcgaggt cacccgcgag
     9481 ctcgccaggc cggcgctcct cacctctctc tccagggcgg actccgcccg cggtgccctg
     9541 gtccaggcct acctcgaggt cctctcggag gtccccgacc tcgatgtggc ggcccgcgcg
     9601 ggccgccagg aggccgagga agtctcccgc atggcacacg gggtcttgaa ggccggcggt
     9661 gtccactcca ggcggggcct cgaagggata gccaacctcg gcggcctgct ccgggcggac
     9721 cctcgcctct ccccgactac gaccgagtat cccgtcatcg ccgcagcctt cctcgtcgcc
     9781 ctcgagtacg gtcctgacgc cctgagccac aggctcagac ccgccacggg atggtgaatc
     9841 gcaatggggc cttaggcccc tttgcgagcc ttcagccatc agccgtcagc cctcagctct
     9901 cttgttcttg ctgagagctg aaagctgaca gcgcgattcg gggagggccg atggccctcc
     9961 ccgaatcgct tgaggtagaa tctggcagaa ctgtcggcga agaggaggag catgagggcc
    10021 ctggtcatcg tgggacacgg ttcgcacctc aacgaagact ccagcctgcc cgtctatgag
    10081 cacgccggcc gcatcaggga gaccggagag ttcgacgagg tcgtcgaatg cttctggaaa
    10141 gaggaaccct ccatgcgcca cgtgctcgac accatagagt cagacgacgt ctacgtcgtc
    10201 cccgctttta tctccgaagg atatttcacc caacaggtca tccccaggga gctcggtctg
    10261 gaagggcccc tctccaggaa gggcgagaag accgtccgct acgccggacc ccttggcacc
    10321 ttcgaggaga tgcccgatgt catcctggaa cgtaccgaag acctcatgcg cggaaaagat
    10381 gtctcgggac gcacggccct cgtcttgctc ggccacggaa ccgacctcaa caagaactcc
    10441 ggtggtgtga tctacctcaa cgcaggacgc atcagggagc gtgggatcta cgacctcgtc
    10501 gaagtggggt ttctggatca agaccccgag ataggcgacg tcgtcgatcg tatcgaggct
    10561 gagaacgtga tcctgatccc gttgttcatc gcggagggct ggcacacccg ggagacgatc
    10621 cccgaggacc ttggccttac gggcgatgtt accctacgcc ccggcaagac gatcttctac
    10681 ggtgcccccg tcggaacgca cccttccatg gcgaacctca tagtcgcccg cgcccggggg
    10741 acgatgaccg ggcagcctgt tgcctgacgc tcacaccggg cgacccgaga acgccgccgc
    10801 gagttcgcgc gaggcctttg cggcatgggt ggacgatgcg ccgcaaggtc gcgtcttcgg
    10861 gcaggtgatc gtacgccctg ctccgcccct cggatactcg ctgcgtcacg tggacgacga
    10921 gagagaggat ggcctcagag tcactgagga cccgcacgct gcgcgggaga tcgccaggct
    10981 taccgagcgc ggggagtaca ggccgctgaa gagctccccg aacctgcggc gcggctggga
    11041 gctcagggtt gcagacgacc gcgctctggc gaccgcgatg aattacctct accccgccgg
    11101 cgtcgtccac tggcacctcc acagggaggg gaggctggat ctcacgagct tcagggagaa
    11161 cgcggcgagg cagagtggga tctacaaacg ggttcagcgg ctctcggacc agggggtgat
    11221 cgacgcggcc acagcctgtt gcgaggactc ggtatgtctc aagaagaccc tctgggacgt
    11281 ggatgagggc acgccgctcg aagtagaccg gggcgaaggt gagatcccct gccccgaacc
    11341 gtgcagcatc ttcgtctcct ttgcgaggcg cgtaagactc ttcgagcggg agaaagacct
    11401 cgaccccaca gatctctcac cctcggagac agaagatctc gccgctctcg tcgaggcagc
    11461 ggccgcgggt ggggtagatc tcgcccgcga agccgagttc gaaagaccgc tgaacgagag
    11521 gaggatgcgc tacaggaggc tcaccctcgt ccccaaacta cgccgcaacg ggtagagagc
    11581 cccatggagt ctccagcggc gtgctggccg ccgtccctgg gctgtttccg aaccggcttt
    11641 tcggggtatc tcgttgtttc gagataccga ctccgcgaag atggaggtag tgaatggcac
    11701 gcagcaagag aaaggccgcc aagcccggtg cgaaggcagg agcaagggtg gcgcccgtgg
    11761 ccggcaggac cgcggggaag gccgcgtgga agctcggcaa ggccgaggcc aagctaatac
    11821 gccgggcgat gagcgcgcag gaatcgaaga agtcccggta cttcaagtac gcgatcttca
    11881 ccctgatcgg gctcgccatc ggggccgcca tcgcccgttc caagaaaggc gagacctcgg
    11941 aaggatcctc gtcctacacg ggcacgaccg gtccccacgc ccccgatccc ggcagcccgg
    12001 caggagagcg gggccagacc tggggtagtg gcaccccggt agggacggcc ggcggcgcca
    12061 accgcaccgg cgccgagcga gactactccg atccctcctc cggacccctg atcggcgaac
    12121 agcatagcga cgtcgagggc gtaggcgagc agcaagagga gttggaacaa cgcatccgca
    12181 cccagatcgg tgaggacccg cgcacggccg agatgccgcg ggtgaacgtc gaggtaaacg
    12241 acggtatcgc cgaactgcgc ggcaccgcgc catccgagga cgccaagcag gccgccggtg
    12301 agatcgccca gggggtcgaa ggcgtccgcg aagtacgaaa cctgataacg gtgagctagc
    12361 gctcaccggg cttcaggctt cagcctgccg ggccacgtct tacggaatag cgcctccgct
    12421 gtggatagcg ggggcgtttt cgtcgcaccc gctagcgtcc gcccgcgcaa cctgacacct
    12481 gatgcctgta acctgatgcc tattctcgaa tgaggctgag caactcgtcg cgtttgcgct
    12541 ccatagtttc tttgtcttcg gcctccacgt tgaggcggag gaggggttcg gtgttcgagg
    12601 ggcggaggtt gaaccaccac tctcccaggt cgaccgtcag gccgtcgagg tagtctacct
    12661 tggcgtcgtc gtgcgccgag aaatggtctt cgaccttctg cagggtctct tcctggtctt
    12721 ctacttcgga gttgatctca cccgagcgga cgtaggggtc gatgggggag aggagtctgg
    12781 agacgggctc ttcctggcgg gcgacgagct cggctatggt cagcatggcg atgatgcccg
    12841 agtcggcgaa gtagttgtcc ctgaagtaga agtgcccgga gtgctcccct ccgaaggctg
    12901 cgttgttctt gcgcatctgg ggtttgatga ttgagtgtcc cgctttggt
//
LOCUS       DATMVF010000129         6574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_518126, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000129 DATMVF010000000
VERSION     DATMVF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518126"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..286)
                     /locus_tag="VN178_06645"
     CDS             complement(44..286)
                     /locus_tag="VN178_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80688.1"
                     /translation="MTVYEIRVKGHLDPRWSEWFDGLRITNEPHGEALLSGNVADQAA
                     LHGVLAKVRDLNLQLISVTSVDSDQQELSGGRGAQE"
     gene            complement(690..872)
                     /locus_tag="VN178_06650"
     CDS             complement(690..872)
                     /locus_tag="VN178_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80689.1"
                     /translation="MYPMDPRLLKQMHEQMVRDAQRKVLPRELRAARKRRTGRASALA
                     WELKRAVSRLLKLLRS"
     gene            complement(988..1575)
                     /locus_tag="VN178_06655"
     CDS             complement(988..1575)
                     /locus_tag="VN178_06655"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HWS80690.1"
                     /translation="MEARLDKDHPVSPGSARPTTVGSAVRSLTLALATLTTGLIAGTF
                     YAYSVSVNLGLAAQPDATYVATMNAINDRIENPLFFLSFFGAAVLPLAMLGYYYRRPR
                     SGRCWLVALASVLYIGGSFMLTSFVNVPMNNQLATVDSDAPARVLATARDAYERPWVF
                     WNGVRTVFSVLAFLALIGACLLREERAPRRGTRTR"
     gene            complement(1753..2151)
                     /locus_tag="VN178_06660"
     CDS             complement(1753..2151)
                     /locus_tag="VN178_06660"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HWS80691.1"
                     /translation="MLRTARLAYRTLAGLFVAGTVTQFFLAGLGVFGAASFGAHATLG
                     TILGIVSLVLLLLAGVLVFAGGLEKRTAGVAALLVVLMVVQYSLVELFSETAPALAAL
                     HPVNGLLVLGVACSLAFGLRPSPSAARSRA"
     gene            complement(2323..2514)
                     /locus_tag="VN178_06665"
     CDS             complement(2323..2514)
                     /locus_tag="VN178_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80692.1"
                     /translation="MMDLQFPKLRHEELLREAENNRQVKVSRATGKRRAGWSSALVWE
                     LRRHTGVLLKILRTLKNVG"
     gene            complement(2559..3044)
                     /locus_tag="VN178_06670"
     CDS             complement(2559..3044)
                     /locus_tag="VN178_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80693.1"
                     /translation="MVGYMSLVEQVDADFSRARRRALLGRMRTRLRNDIASDGLLCFD
                     DLRRTPGAVGRIHRGIRTVPVGQVGGSVGRCSDFDRDFMPAKANVGERWERIDRAFWR
                     GKELPAVILYKVGSFYFVLDGHHRVSVAQYHGVEWMDADVTEFGGVGEVWSEQRDRKS
                     A"
     gene            3393..6116
                     /gene="malT"
                     /locus_tag="VN178_06675"
     CDS             3393..6116
                     /gene="malT"
                     /locus_tag="VN178_06675"
                     /inference="COORDINATES: protein motif:HMM:NF003420.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HWS80694.1"
                     /translation="MPDALVTTKVRVPRTRPELVPRPRLRETLVRYEGRRLILVSAPA
                     GFGKTTLLSEWLEDRSGDGRPVAWLSLEEADNDPARFLAYLVSALQSALGEEIGEGVL
                     ASLRLPEFPPVESVVGVLINELADVQREEVTIVLDDYHVIHSGPIHEATTFLLEHLPE
                     DVHLVISGRADPPLPLAKLRARDQMTEIRAAELRFTTEEATAFLKGVMGLVLSAADVA
                     ALEEVTEGWIAALQLAALSMRDREDLSGFVESFSGSNRHVLDFLAEEVLERQPEGVRG
                     FLLDTSVLERMSAPLCDALTGRDDGQEMLERLERENLFVVALDEERRWYRYHRLFADF
                     LKARLERESLERIKELHRQAAGWYEWNGWTSEAVEHALAAGDVEWASQLVEYNALAFV
                     LRGEGATMDRWLSALPTGVVRARPRLSLARAIWALISGRVDEVEPLLTDAERAADDQP
                     HEPPVEEADSRLANFPGTIAQLRAELARQRGDAESAMQFAQRALAHAGEGDRYLRFLS
                     RWNIAVATLMQGRLGVAEQALAELVRDPWATGPNHFFAVRACYTLGQAQCGQGRLRAA
                     LETYRQGLELAVEAGRLPMPTAGMPHVGLAEILCEQNNLDGALEHATEGVVLCRQLGY
                     AQQSVTSLSVLAWIRQARGDQAGALEAIGEAERLVPNPKLLVDINFPAAVQRARLLLA
                     QGKVDDAARWCAERGLGVEDELSYLREREHLVVARVLLAQRNPDQALRLLERLREEAE
                     AADRTGSVIEILALQALALWAEGKKERAVSSLTRALTLAESDGYIRTFVDEGPAMGEL
                     LSATLQARQRRRLDPAGRVSVSYLAQLIAALAQEDASAPVANERLSEPLSERELEVLA
                     LVAAGKSNRQIASELFVALSTVKTHIRNIYDKLDARSRTQALARARELELL"
     gene            6320..>6574
                     /locus_tag="VN178_06680"
     CDS             6320..>6574
                     /locus_tag="VN178_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80695.1"
                     /translation="MQTENTQDMKTTLVLGGTGKTGRRVAARLEARGLPVRVGSRSGQ
                     PPFDWENLDTWAPALEGVESVYVSYYPDIAIPGAPEAVRSF"
BASE COUNT         1181 a         2151 c         2045 g         1197 t
ORIGIN      
        1 ctgtcggtgt cggagaggga gtgtccgagg cgggcgtagg tactcattct tgggctccgc
       61 ggccaccaga gagctcctgc tggtctgaat cgacgctggt taccgagatg agttgcaggt
      121 tcaggtcacg caccttggcc agcacgccgt gcaaggcggc ctggtccgca acattccctg
      181 agagcagcgc ctctccgtgc ggctcgttgg tgatccgaag tccatcgaac cactccgacc
      241 agcgtggatc gaggtgccct ttcacccgaa tctcgtaaac ggtcattcgt ctctcctttg
      301 tcgcgcttgc tatcggcgat cccgaggttt gctgcgactg agcctgctgg atcagcggcc
      361 accggcatcc ggtctaatga cctgcttaag gtacggacaa acataggtcc cttcgtgccg
      421 ccgtacaggg accatgaaga gagcagaggc agcgtcccga tgcgctctac gtagtgcccg
      481 agcgcgacgt caacgagtgc gggcaccttc gacgcatctg ccccggttgc gggtcggaac
      541 gaatactcaa ccacttctca accaccggct ccctctataa ttcatcgctt ctctccttgt
      601 gcactgtcgt attggccccc atcacaggcg ggatatcctg atcgatattc gtcattaccc
      661 gacgcctccg cgttcctaga gggctcatct taggacctca ggagcttgag gagacggctg
      721 acagccctct tcagctccca cgccagggcc gatgcccgac cggtgcgccg cttgcgagct
      781 gcccgcaact ctctgggcag gacttttctc tgcgcgtcgc gcaccatctg ctcgtgcatc
      841 tgcttcaaca gtcgggggtc catcgggtac atcttcggtc ctccgtgctt gtgctcgggt
      901 ggtgacggat cggacggtct tcgacctttc ggtccctcgg cagcaaggca cggaactccg
      961 tcaccacagc gtctatccac ccgacgccta tcgcgtgcga gtcccacgcc gcggagcgcg
     1021 ctcctcccgc agcaggcagg caccgataag agccaggaat gccaggactg agaacacagt
     1081 tcgcacgccg ttccagaaaa cccacggcct ttcgtaggca tcccgcgccg tcgccaggac
     1141 ccgtgccggg gcgtccgaat ccacggtagc gagctggttg ttcatgggca cgttcacgaa
     1201 ggatgtcagc ataaagctgc ctccgatgta gagtacactg gccaacgcga ctagccagca
     1261 tcgcccggag cgcggccggc ggtagtagta cccgagcatc gccagcggta acacggcggc
     1321 accgaagaag ctcaggaaga acagcggatt ctctatcctg tcgtttatcg cgttcatggt
     1381 ggcgacgtag gtagcgtccg gttgcgccgc gaggccgagg ttgaccgaga ccgagtaggc
     1441 gtagaaggta ccggcaatga ggccggtggt gagggtagcc aaagccagcg tgagggaccg
     1501 gacggccgag ccgacggttg taggccgggc acttccgggg cttacagggt ggtctttgtc
     1561 gagtctcgct tccattccga gttctcctcg tctttgcgtc gtgggtaccg actttgatct
     1621 ttgtgcgcaa tcgccggatg tttcgaggac cggctggcca tgctccgtca ggttaagtaa
     1681 ccgtccggga ggtctcgctc cgcctggcgt ggggataggg gtattcggcc ccgtagccga
     1741 gcagacgcgc gatcaagctc ggctccgcgc ggcggacggg gacggacgaa gcccgaaggc
     1801 gagggagcag gccacgccca gcaccaagag gccgttcacc ggatgcagcg ccgccagggc
     1861 cggggccgtc tccgagaaca gctccaccag agagtactgc acgaccatga gcacgaccag
     1921 gagtgccgcg acccccgccg tgcgcttttc cagccctccg gcgaagacca gaaccccggc
     1981 cagcaacagt aggaccagcg agacgatccc caggatggtg ccgagcgtgg cgtgcgcccc
     2041 gaagctcgct gccccgaaga ccccgaggcc ggcgaggaag aactgcgtca ccgtcccggc
     2101 gacgaacaac ccggcgaggg ttcggtaggc caaacgtgcg gttcgcagca tcccttcgat
     2161 cctttctctc tggcgcgtcg gacgcccgcg atcatttcct tactgcaata gcgcgactcc
     2221 atccctcccc ggcagtcctc ccgaatacag cccgcaatcc cgtaaccgga cttagtggtt
     2281 cgtcgcctgc tcttgccacg ccttgtcctc cctgccccat ttctagccga cattcttcaa
     2341 ggttctcagg atcttgagga ggactccggt gtgtctcctt agctcccaca ccagagccga
     2401 gctccagcca gcacgccgtt tgccagtcgc ccgcgacact ttgacctggc gattgttctc
     2461 cgcttcgcgc aatagctcct cgtgacgtag ctttggaaac tggagatcca tcatctgtta
     2521 cctcctctgt tcgttcctcc agctcgtgga tggctagatc aagcactctt cctgtccctt
     2581 tgctcactcc acacctcccc aacgccaccg aactccgtca cgtcggcgtc catccactcg
     2641 acgccgtgat attgggctac cgagacgcgg tggtggccat ctaagacgaa atagaagctt
     2701 cctaccttgt agagaattac ggcaggtagc tccttgcccc tccagaacgc ccggtctatc
     2761 cgctcccacc tctcccccac attcgccttg gcgggcatga agtctctatc gaaatcggaa
     2821 cagcggccta cgctgccccc tacctgacca accggcactg tccttatgcc tcgatggatt
     2881 cgtcctactg ccccaggggt ccttctaaga tcatcgaagc agagcagccc atccgaggca
     2941 atgtcattcc gcaagcgggt cctcatcctc ccaagcagcg cccttcgccg ggcgcgggag
     3001 aagtccgcgt ctacctgttc cacaaggctc atgtaaccaa ccatcgacga aatcctttcc
     3061 tcggtacttt cagatgccac cgtagccggt caatagctca gcctacgttc cataccgtgc
     3121 gccgtgtcct cgatcatctt cataccgccc ggccgggtgg agcggcatca cccgaaagga
     3181 ggattagggg gaggacatcc gactttgccg cggttcactc ctgcgccggc ggatatgatc
     3241 ggcgcccaac gtctgttgac gactccgtgc tgccgtgcac gctccaatga agttgggcct
     3301 cgatgggatc agggacggta aaagagcaca acttgacctg gcttcttttc ggagtaagaa
     3361 taggctcagg ctgacccgtt ggagcatctg ctgtgcccga cgcgctcgta acgacgaagg
     3421 tgcgcgtccc caggacgcgc ccggaactgg tgccgcgccc tcgcctgcgc gagaccctcg
     3481 tccgatatga gggacgcagg ctgatcctcg tctctgcccc ggcaggattc ggcaagacca
     3541 ccttgctgag cgaatggtta gaagaccgtt cgggggatgg cagacccgtt gcctggctct
     3601 ctttggagga ggcggataac gacccggcgc gtttcttggc ttacctggtg agtgcgcttc
     3661 agtccgcact aggagaggag atcggcgagg gggttctggc ctcgctgcgt ttgccggagt
     3721 tccctcccgt cgagtcggtg gtgggagtct tgatcaacga gctggccgac gtacaacgcg
     3781 aggaggtcac catagtccta gacgactacc acgtgatcca ctccgggccc atccatgagg
     3841 ctaccacttt cctgctggag cacctgcctg aggacgtcca cctcgtcatc tccggccgcg
     3901 cggatccgcc cctccccctc gccaagctgc gcgccaggga ccagatgacc gagatccggg
     3961 ccgccgagct gcgcttcacc acagaagagg ctactgcttt cctgaaaggc gtgatgggac
     4021 tcgtcctttc ggcagcggac gtggcggcgc tagaagaggt cacggagggt tggatcgccg
     4081 ccctccagct ggcagccctc tcgatgcgcg accgcgagga cctctccggc ttcgtcgagt
     4141 ccttctccgg cagcaaccgc cacgtcctgg acttcctggc cgaggaggtc ttagagcggc
     4201 aacccgaagg ggtgaggggg ttcctgctcg acacctccgt gctggagcgc atgagcgccc
     4261 ctctgtgcga cgccctcacc ggccgcgatg atgggcagga gatgctcgag aggctagaga
     4321 gggagaacct cttcgtggtg gccttggacg aggagcgtag atggtaccgc taccaccgcc
     4381 tcttcgccga cttcctgaag gctcgcctgg agcgtgaaag cctcgagcgg atcaaagaac
     4441 tgcaccgcca ggcggcgggg tggtacgagt ggaacggttg gacctccgaa gcagtcgagc
     4501 atgcgctggc cgccggagat gtcgagtggg cgagccagct ggtcgagtac aacgccttgg
     4561 cgttcgtcct gcgcggcgag ggtgcgacaa tggaccggtg gctgtcggca ctgcccaccg
     4621 gggtggtccg tgcccggcca cgactgtctc tggctcgggc gatctgggcg ctcatcagcg
     4681 gccgagtgga cgaggtcgaa ccgctgctca ccgacgccga gcgcgcggcc gatgaccaac
     4741 cgcacgagcc gccggtcgag gaggcggaca gcaggctggc gaattttcct gggacaatag
     4801 cccagctgcg tgccgagctc gcccgtcagc gtggcgacgc cgaaagcgcg atgcagttcg
     4861 cacagcgggc ccttgcccac gcgggggagg gtgaccggta cctgcgcttc ctctcccgct
     4921 ggaatatcgc ggtcgcgacg ctgatgcaag gccgtctcgg cgtagcagag caggcgctcg
     4981 ccgaactggt ccgcgaccca tgggcgaccg ggccgaacca tttcttcgcc gtgcgtgctt
     5041 gctacaccct tggccaggca cagtgcggtc aaggtcgtct ccgcgctgcg cttgagacct
     5101 accggcaggg gctcgagctt gcagtcgagg ccggccgact gccgatgccg acagcgggca
     5161 tgccgcacgt gggactggcc gaaatcctgt gtgagcaaaa caacctggac ggtgcactag
     5221 agcacgccac cgagggcgtt gtgctttgcc gacaactcgg atacgcgcag cagtcggtca
     5281 cgagcctgag cgtgctggcc tggatccgac aggcgcgggg cgaccaagcc ggcgctctgg
     5341 aggcaatcgg cgaagccgag cggctcgtgc cgaacccgaa gctgctcgtc gatatcaact
     5401 tcccggcggc agtgcagcgg gcccggctgc tgctcgccca gggcaaagtg gatgatgccg
     5461 ctcgctggtg tgccgagcgt gggctgggtg tggaggatga gcttagctac ctccgagagc
     5521 gcgagcacct tgtcgtggcg cgggtgctgc tcgcccaacg caatccagac caggcgctga
     5581 ggctgctcga gcggctgcgt gaggaagcgg aggcggcgga caggacgggc agcgtgattg
     5641 agatcctggc gctgcaagct ctggccctgt gggcagaggg caagaaggag cgggccgtca
     5701 gctccttaac gcgagccctc accctcgcag aatccgacgg ctacatccgg actttcgtgg
     5761 acgagggacc cgcgatgggt gaactcctct cggcgacgct ccaagcccgc cagaggcgtc
     5821 gcctcgaccc cgcgggccgt gtctcggtct catacctcgc acagctcata gcggccctcg
     5881 cacaagaaga cgcgtcggca ccggttgcca acgagcggct ctccgagccc ctcagcgagc
     5941 gggaactgga ggtgctcgct cttgtcgccg ctggcaagtc caaccggcag atcgcctccg
     6001 agctcttcgt cgccttgagc accgtcaaga cccacatcag gaacatctac gacaagctgg
     6061 acgcccgtag ccgcacgcag gccctagcgc gtgccaggga gctggaactg ctttgaccgg
     6121 accgcagggt cttcgaagaa tctcccggca cttccgtgat tcgttgtcct aggcggtaac
     6181 cacggcctgc gtaagcccgt gacgatccat gcacaaccat ctcctccccc ctgatcctcc
     6241 tttcgggtga tgccgctcca cctgaccggg cggtatgtct ctaacgcgag taaagcgtac
     6301 cccgcaaaag gaggagaaga tgcagacgga aaacacccag gacatgaaga cgacgctggt
     6361 tcttgggggt acgggcaaga ccggtcgaag ggtggcggcg aggctagagg cgcgtggact
     6421 gcccgtgcgg gtgggctccc gctccggcca accgcccttc gactgggaga acttggacac
     6481 atgggcaccc gcgctcgaag gagtggagtc tgtgtacgtc tcctactacc cggacatagc
     6541 gataccgggc gctccggaag cggtgcgctc gttc
//
LOCUS       DATMVF010000130         6721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_242938, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000130 DATMVF010000000
VERSION     DATMVF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242938"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VN178_06685"
     CDS             <1..1185
                     /locus_tag="VN178_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWS80696.1"
                     /translation="VWRSKPYPLPPLRPFRTSEIFSKATKEMGLHPYPTPVAVNSEPY
                     NGYPATTYCAWSGGFGPFNDERWHPGLTSVPEALATGNLDLRTQCRVVRVLTDSTGHA
                     SGVEYVDANGNPRVQEARTVILCGYTFENVRLLLLSGDQRHPGGLGNNANQVGKHFMT
                     KMWADVYGFFPDVVFNKHTGPAAQMWSLDDFIAADFDSAAHGFIGGATPNIENQQLPI
                     QISREGLPPDVPSWGKGYKDHLRRWQHVCAIRLQPESLSYQTDFLDLDPRHRDRSGLG
                     LPLVRITCDLRENERRLADWMEIEAENILHRMGAAKTWRGSRFRGVCSSHDLGGCRMG
                     EDPASSVVDPDLRVHDTPGLYVFGGAVFPTCPGVNPTLTMWALCYRATEHLVSRLRRG
                     DE"
     gene            complement(1588..1782)
                     /locus_tag="VN178_06690"
     CDS             complement(1588..1782)
                     /locus_tag="VN178_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80697.1"
                     /translation="MTHHTNDLSLPEVERLVDAANALRRQRGASGSAMESGDHRAERR
                     LDSLFRTSHTLAVYGTLAPG"
     gene            complement(1779..2027)
                     /locus_tag="VN178_06695"
     CDS             complement(1779..2027)
                     /locus_tag="VN178_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80698.1"
                     /translation="MRNPNEAIYLRVGELSPSVTPETDARTGAIRLAREGVTCFGTGQ
                     LLFVLPELAVEAVGQAATEFGTHRCGPAFDRGWKEDTP"
     gene            complement(2024..2665)
                     /locus_tag="VN178_06700"
     CDS             complement(2024..2665)
                     /locus_tag="VN178_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HWS80699.1"
                     /translation="MRSRERAKASRLLADFGTATVCEAAGGEGIVDVDLKQVVPGSRV
                     AGPARTVLCGQGDNLAVHEALAAVRPGEVLVITMPEPAPFGAVGELIVIQARACGAAG
                     MLVDVAVRDVAELKEIGLPIWARFVRVRGTSKTDPGRLDVPVVVGGATIRPGDVVVMD
                     DDGAVVVPAERVEEVSEGARAREDKESRLRPKLRAGELTYDLLGLRAKRGDGG"
     gene            complement(2662..3393)
                     /locus_tag="VN178_06705"
     CDS             complement(2662..3393)
                     /locus_tag="VN178_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWS80700.1"
                     /translation="MGEAGSGGRKLLIVGAHSGDFVWRAAGAIAVATAGGGEAKVVAL
                     SYGERGESGDLWDEPDQTVENVKRVRHEQSEEAARAIGARFECWDLGDYPLRIAEGTV
                     GRLADAIRGFAPDVILTHTERDPFNPDHPVAHAAALRARLLASGAGVASAFKTVKPPE
                     LLYFEPHQPELCGFQPDTFVDITGVFERKLEAMEAMAAQKYMHKHYAERAEHRAFQAR
                     YATGDGEVRHAEAFQRAIPQVVKSL"
     gene            3716..4600
                     /locus_tag="VN178_06710"
     CDS             3716..4600
                     /locus_tag="VN178_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWS80701.1"
                     /translation="MKPSGKLMAAILTLVLGALTLTGCGGLGQNDGLLLANIGWDENV
                     AVSNLSKVILEDELGYESVEIRTNDNLDVTYRDVASGEVDAFQDVWLPNQEDLLDQVA
                     EEVEHLDPWFLGETRQGMAVPAYMDVKSIPQLNGTDAELILGIEPSSVMLQEVGEEVI
                     PSYGLEQKLVAGPTAGMLAEVESLYTFKEEFVFLAWSPHWMNQRFDIRYLEDPKDAMG
                     PTNDPAECSTIVRRDLREEDPVAYAFMDALELTEEQINDLEYTINKEDDPLAGARRWA
                     SENREVVRPWIAAARNAM"
     gene            complement(4919..5677)
                     /locus_tag="VN178_06715"
                     /pseudo
     CDS             complement(4919..5677)
                     /locus_tag="VN178_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(5981..6106)
                     /locus_tag="VN178_06720"
     CDS             complement(5981..6106)
                     /locus_tag="VN178_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80702.1"
                     /translation="MALPELTPQLALLEEAVTILFCQVDEAYYRLDPKGRHYETL"
     gene            6208..>6721
                     /locus_tag="VN178_06725"
     CDS             6208..>6721
                     /locus_tag="VN178_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS80703.1"
                     /translation="MQSCTLIGLKSTGVLAMHKKYVVRLTEEERSRLETLVRRGRAHT
                     RKLLYARILLKADANGPDHWTDERIAKAFEISTATVARERRRYCEDGLEVALMPKKPG
                     RPRRRVLDGRAEAHLLALACSEAPEGRERWSMRLLADRMVELGHVEALSYETVRRALK
                     KTASNLTSSAS"
BASE COUNT         1281 a         2198 c         2046 g         1196 t
ORIGIN      
        1 gtctggcgca gcaaacccta cccgctgcca ccactgagac ccttccgtac gagcgagatc
       61 ttctccaagg ccacgaagga gatgggtttg catccctacc cgacgccggt cgccgtaaac
      121 agcgagcctt acaacggtta cccggcgacc acttactgcg cctggagcgg gggttttggt
      181 cctttcaacg acgagcggtg gcaccctggc ctgacttcgg tccccgaggc gcttgcgacc
      241 ggcaacctcg acctgcgaac ccaatgcaga gtcgtccgcg tcctcaccga ctcgaccgga
      301 catgccagcg gcgtcgagta cgtggacgcc aacgggaacc ctcgagtgca ggaggcgcgt
      361 acggtgatcc tatgtggtta cacctttgag aacgtaaggc tcttattgct ctcgggggat
      421 cagcggcatc cagggggtct gggcaacaat gcgaaccagg tcggcaaaca cttcatgacc
      481 aagatgtggg ccgatgtcta tggattcttc ccggacgtgg tcttcaacaa gcacaccggc
      541 ccggcggcgc agatgtggag ccttgacgat tttatagccg ccgacttcga ctcggcagct
      601 catgggttca ttggcggtgc aacccccaat atcgaaaacc agcagttgcc gatacagata
      661 agccgcgagg gcttaccgcc tgacgttccg tcctggggca aggggtacaa ggatcacctt
      721 agacgctggc agcacgtctg tgcgattcgg ctccagcccg agtcgttgtc ctatcagacc
      781 gacttcctcg acctggaccc tcgacaccgt gaccgcagcg ggctagggct accccttgtg
      841 cgcatcactt gcgatctgcg agagaacgag aggcgccttg ccgactggat ggaaattgag
      901 gcggaaaaca tccttcacag gatgggtgcg gcgaaaacat ggcgtggttc gcgctttcgt
      961 ggcgtctgca gcagccacga cctcggcgga tgccgcatgg gtgaagaccc cgcctcctct
     1021 gtggtggacc ccgatctgcg cgttcacgac acgccaggac tgtacgtctt cggcggagct
     1081 gtctttccaa cctgccccgg ggtcaatcca acgctgacga tgtgggctct ctgctaccgg
     1141 gctaccgaac acctcgtgag tcgccttcga cgtggtgacg aatagtgatg aggttgagag
     1201 cgagaactac cggacttgcg tcgctcggcc gatcgcggac gagagacatg cgaggtctgg
     1261 ggctgaccac ccggaccggc ttcggccgcg gcgtagaggt tggccacctt gtacagtcgc
     1321 cgctcgcccg cctgcccgcg gccaggcccc gtgccgaaca ccggcaccag gatgcgctcg
     1381 tatcccgggc cctcaaagcg gtcgaggtcg ggccaagcga ccgccaacga gggggcggtc
     1441 aacacccgca ccgccacggc gtctcccccg acccggggac ggaacccggg aaagccgagg
     1501 gccgcgcccc accccagcgg gatcagatgc ccctcgatca gaccgtctgt ccactcgccc
     1561 ccaagggcgc gaggacgtgg tggttggtca gcccggggcg agcgtaccgt agaccgccaa
     1621 cgtgtggctg gttctgaaga gggaatcgag ccgccgctcg gcacgatggt cgcccgactc
     1681 catcgcgcta cccgatgcac cgcgctgccg acgcagcgcg tttgccgcgt ccaccaaacg
     1741 ctccacctct ggcagggaca gatcgttcgt atggtgtgtc acggcgtgtc ctccttccac
     1801 ccacggtcga acgccgggcc gcagcgatgg gtgccgaact ccgtcgccgc ctgtccgacc
     1861 gcctcgaccg cgagctccgg gagcacgaaa agcagttgcc cggtgccgaa gcaggtcacg
     1921 ccctctctcg cgaggcgtat ggccccggtc ctggcatccg tctccggcgt gaccgatggg
     1981 gagagctcgc ccacccgcag gtagatagcc tcgtttgggt tcctcatcct ccgtctcctc
     2041 tcttggcacg tagtccgagc agatcgtagg tgagctctcc cgcccgcagc ttgggcctga
     2101 ggcgagactc tttgtcttct cgcgcccgcg ccccctccga gacctcttcg accctctcgg
     2161 ccgggacgac cacggcgcca tcgtcgtcca tgactacgac gtccccgggg cggatcgtcg
     2221 cgcctccgac cacgaccggg acgtctagtc tgccggggtc ggtcttactc gttccccgca
     2281 cgcgcacgaa cctagcccag atggggagtc cgatctcctt aagctcagca acatcgcgca
     2341 ccgccacgtc cacgagcatt cccgccgcgc cgcaggcccg tgcctgaatg actattagct
     2401 caccgacggc accgaacggc gccggctcgg gcatggtgat caccagcacc tcccccggcc
     2461 ggaccgcggc cagggcctcg tgcaccgcca ggttgtctcc ctggccgcac aggacggtgc
     2521 gcgctggccc cgctacgcgg ctgccaggga ccacctgctt gaggtccaca tccacgatcc
     2581 cctcaccgcc cgcggcctca cagaccgtcg ccgtaccaaa atcggcgagg agacgcgaag
     2641 ctttcgcccg ctcgcgcgat ctcataacga cttcaccacc tgcggaatgg cccgttgaaa
     2701 cgcctcggcg tggcggacct cgccgtcccc cgtggcgtac ctggcttgga aggcgcggtg
     2761 ctcggccctc tcggcgtagt gcttgtgcat gtacttctgt gccgccatcg cctccatcgc
     2821 ctcgagcttg cgctcaaaga cgccggtgat gtccacgaac gtgtccggct ggaagccgca
     2881 aagctccggc tggtgcggct cgaagtagag cagctccggc ggcttgacgg tcttgaaggc
     2941 gctcgccacc cccgccccgc tggccaagag cctcgcgcgc agcgcggcgg cgtgggccac
     3001 cgggtggtcc gggttgaacg ggtcacgctc ggtgtgggtc agtatcacgt caggggcgaa
     3061 ccccctgatc gcgtcggcca accgcccgac cgtcccttcg gcgatcctca aggggtagtc
     3121 gccgaggtcc cagcactcga aacgggcccc tatcgccctg gcggcctcct ccgactgctc
     3181 gtgcctgacc cgcttcacgt tctcgaccgt ctggtccggc tcgtcccaaa ggtcccccga
     3241 ctcgccgcgt tcgccgtagg agagcgccac caccttggcc tcgccgcccc cggcggtcgc
     3301 caccgcgatc gctcccgccg cccgccacac gaagtcgccc gaatgcgccc ccacgatcag
     3361 tagcttcctg ccgccgctac cggcctcgcc catctgctca gcctttcttt caccagcgtc
     3421 ctcgaacgct ccaaaagatc attggcgcac gatgataacg tacttaaggt tcgatcgcgt
     3481 cgaaggacag ctcctcgctc cacaccagcc cgcttccggg accaggtcct caagcgagag
     3541 ctcgtcgggt agcggggaga aagagcggat cttggtgccc attatgacgg cgcgtgacct
     3601 cctgtacgga cgggaagccc gcaggccata agactccgga gcaagagtaa ctgtggtagt
     3661 gtgaggcggc aggcggttct acgatgaaca ggcaaaactg cgggagggaa caacggtgaa
     3721 gccgagcggc aaacttatgg cggcgatcct cacacttgtg ctcggcgccc tcaccttgac
     3781 tggctgcggc ggactcgggc agaacgatgg gctcctgctc gccaacatag gttgggacga
     3841 gaacgtggcg gtctccaacc tgtcgaaggt tatcctcgag gatgagctcg gttacgagag
     3901 cgtcgagatc cgcacgaatg ataatctcga cgtgacctac cgagatgtgg ccagcggcga
     3961 ggtggacgcc ttccaagatg tgtggctgcc caaccaggag gacttgctcg accaggttgc
     4021 cgaagaggta gagcacctcg atccgtggtt cctgggggag accaggcaag gtatggccgt
     4081 ccctgcctac atggatgtga agagcatccc ccagcttaac ggcaccgatg cggagttgat
     4141 cctcggcatc gagccgagct cggtgatgtt gcaggaggtc ggcgaagagg tcatcccgtc
     4201 ttacggcctc gagcagaagc tcgtggcagg tcccaccgcg ggcatgctcg ccgaggtcga
     4261 gagcctttac accttcaagg aggagttcgt cttcctcgcc tggtcaccac actggatgaa
     4321 ccagaggttt gacatcaggt acctggagga ccccaaggac gccatgggcc cgacgaacga
     4381 tccggctgaa tgctcgacga tcgtgcgcag agacctgcgg gaggaggacc ccgtagccta
     4441 cgcgttcatg gacgcgctgg agctgactga ggagcagatc aacgacttgg agtacacgat
     4501 caacaaggaa gacgaccctc tcgcgggggc aaggcggtgg gcatcggaga accgcgaggt
     4561 cgtgcggccc tggatagcgg cggcgaggaa cgccatgtag cccgagggca gccgcatcag
     4621 caccgaggag ttagagcaac aaggctattc cccacaggtg cattcatgcc ctacggaacg
     4681 aagcatcact agcgcagggg cttcggagaa cgccgttcgt gcacaaagaa gccggggccc
     4741 gaccattagg gccccggctt cttgccgatc aacttcagtc tattggcttc ttcggaccat
     4801 gtcggtgcaa atggtcctta gagaatcaac tccgctgcgt caaagggggg acattgcgcc
     4861 ggagaccttc ttgggcgtat acctatgcct cctggtagcc acctgcgagt gctgtccctt
     4921 accgcgctgt gtagcctgcg tctactagat ggtagctgcc cgtgatgaac gaagcatcgt
     4981 ccgaggcgag aaaggccacg aggttagcta cctcttctga tgtgcccagg cgtccgaggg
     5041 cgtggagcat ggctatctgc gcctgggcct ctgcaggaag gtttgcttcc agcaggggtg
     5101 tggagatgaa accggggcct acggcgttga tacgaacccc cttcgcagcg tgctcgaggg
     5161 cagcggcctt cgtcaagccc acaaccccat gcttggacgc cacgtaggca ggcgaattct
     5221 ctattcagac gctacccagc acagatgaca cgttgatgat agagccgccg cccgcccggg
     5281 atattgggcc gatttcggcg cgcatgcagt agaagacgcc atcgagattg accgacatca
     5341 ctcgacgcca ggcctccacg tcgtactctc cggtcggctg agatgttcca ccgatgccgg
     5401 cgttgttgac ggcgatgcgc agtggaccga gttcttcctc agcccgccct accatagcct
     5461 caacctcgtc cagtttgctc acatctgcgg cgtgagggag agcagtgcct ccgtcttgtg
     5521 agatagcgct ggccacactc tgtgcgcgtt ctttatctag atccacaacc agcacggcag
     5581 caccgctgga agagagcaca tgagcacagg cttcgccgat acctgctcct gctccagtaa
     5641 ctatcgctgt acgaccgtcg agttcatatt tcgccatctg acctccttgt acacgacgtt
     5701 gttgatcact tcgccgaaag cgactacgcc ctgggaactg gtaagaacca cagcagtttg
     5761 catcctgtct gacttcttgt gtccctctta ccgcgaagga ccagtccaca ctcctgagct
     5821 tgcgtacgcg acgatggaac gaggaggggt gaagtccgac ggcttcgggg aacaggtgcc
     5881 agaagaagcg cccaacctcg cgcaagaagg aacgctcgac ctcaacaccc cgaagctgct
     5941 gtaagagggc aagcgttatg acctccgagt ccgagagctc ctagagggtc tcgtagtggc
     6001 gtcctttggg atcgagccgg taataagcct cgtccacttg gcagaacaaa atagttacgg
     6061 cctcttccag gagtgccaat tgtggggtaa gctcggggag ggccatctga agctcctttg
     6121 gacggggaac tctgatggcc ctcgctttac cttacaaacc acgcaacaca catctagtcc
     6181 tctgtcacag ataggttgca tagtaggatg cagtcttgta ccctcatcgg cctcaagagt
     6241 acgggggttc tcgccatgca caagaagtac gtcgttcgcc tcaccgaaga ggagcgcagc
     6301 cgactcgaaa cgctcgtccg tcgcggcaga gcgcacaccc gcaaactgct ctacgctcgc
     6361 atactgctca aggccgacgc taacggacca gatcactgga ccgacgagag gatcgccaaa
     6421 gctttcgaga tcagcactgc cacggtggcg cgggagaggc ggcgctactg tgaggacgga
     6481 ctcgaggtag cgctcatgcc aaagaaaccc gggaggccca ggaggagggt cctggacggc
     6541 agggctgagg ctcacctgct cgctttggcc tgctccgagg cgcccgaagg tagagagcgt
     6601 tggagcatga ggctcttggc cgaccggatg gtcgagctgg gccatgtaga ggcgctctcc
     6661 tacgagaccg ttcgtcgggc actcaaaaaa actgcctcaa acctcacctc aagcgccagt
     6721 g
//
LOCUS       DATMVF010000131        19392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_342857, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000131 DATMVF010000000
VERSION     DATMVF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19392
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342857"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VN178_06730"
     CDS             complement(<1..297)
                     /locus_tag="VN178_06730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80704.1"
                     /translation="MRDLVIRRCEPEDYKAVHLVYSSPRAMAETLGVPYSSEQAWREE
                     LVRERDDSFALVACVNGEVVGHLALSAYTNPRTRHSGHFGIAVRDDWQGRGIGTA"
     gene            469..1458
                     /locus_tag="VN178_06735"
     CDS             469..1458
                     /locus_tag="VN178_06735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS80705.1"
                     /translation="MDQRNLGGEGLVVSELGLGCMGMSEFYGAGDEEESTATIHRAIE
                     LGITFLDTADMYGPFTNERLVGKAIAGRRDEVVLATKFGNVRGEDGSFHGVSGKPDYV
                     REACDASLSRLGVDHIDLYYQHRVDPETPIDETVGAMKELVEAGKIRYLGLSEAGAET
                     IRKAHAVHPISALQSEYSLFTRDVEDEILPTVRELGIGFVPYSPLGRGFLTGRWRSIE
                     DMPEDDTRSARFPRFAEENFKKNLELADRVREVAEGKGITPGQLALAWLLAQGDDIVP
                     IPGTKRREYLEENAAGAGVTLTEDDLAGIEEAMPRGSAAGERYAEEQMRAVGR"
     gene            1468..2067
                     /locus_tag="VN178_06740"
     CDS             1468..2067
                     /locus_tag="VN178_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565683.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HWS80706.1"
                     /translation="MTDVEHISRKGTGVPVSWFPIPEESELPDELQGLFRKAREMVGF
                     VPNVFRAYSYRPERLSAWFAHYRQLHEPTPNLDAAEREMIAVAVSMANGCLYCLVAHG
                     AALRQALGDPILADRITLDHRRAGLDERRTAILDFAVKITERPLDCDPKDLERLRGYG
                     LTEEEVWDVIEIASMYNFTNRMSLACGMIPNEEYHALAR"
     gene            2090..2341
                     /locus_tag="VN178_06745"
     CDS             2090..2341
                     /locus_tag="VN178_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80707.1"
                     /translation="MLQVISVFGSLSILAAFVANQRGWISPSQLSYAAANFVGAVILT
                     VVAIVDQQVGFILLQGTWTLVSLIGIVRILRGDESRRVR"
     gene            2353..2604
                     /locus_tag="VN178_06750"
     CDS             2353..2604
                     /locus_tag="VN178_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80708.1"
                     /translation="MLQAVSVVGALLILLAYAANQFGWTDTSSLRYQIANFAGSTILT
                     VVAVIEVQIGFILLEGVWALVSAWGIVSVLRGGRSGDTN"
     gene            2666..2866
                     /locus_tag="VN178_06755"
     CDS             2666..2866
                     /locus_tag="VN178_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80709.1"
                     /translation="MSGVKYVSEVKVEPVEGKIRRAYLPVQDDPVVFGVHSEVAEHYG
                     VPPEEVEPYDTTLDYVVAAAGG"
     gene            2867..3130
                     /locus_tag="VN178_06760"
     CDS             2867..3130
                     /locus_tag="VN178_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWS80710.1"
                     /translation="MLGTFSGALAARQVSFDNLHAQTVGEIEVERKVLVIKRIKQTFH
                     LTAAESDRETIERVLEVYADSCPVAASVKGSIEISSELDFTAA"
     gene            complement(3139..4614)
                     /locus_tag="VN178_06765"
     CDS             complement(3139..4614)
                     /locus_tag="VN178_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80711.1"
                     /translation="MTELKQTPGEPIACLTISADAGFLPPVIEFVKQMTRRLGLGDVE
                     PVDRAVELVCLNVIENAFGSEEKGFLDLEILRRPGHVVVAVEDQGLPFDYKRLQDGED
                     RTVLETLHRSFDETRFVNLGRSGNRVELLKHLPHTDVRDHLPEEEHHRTVEATAAHED
                     IPLEIRMMHPEESFELSRCVYRSYGYSYDWDYIYFPDRIRELQEQGLMVSCVAVTPED
                     EFVGHLAITVERPGSPVGESGQAVVDPRFRGHHLFPKMKTFMARWAADAGMYGLYSEA
                     TTVHPYSQRGNLHLGARETGFLLGYIPASVSYKQIGDERADRRGSVALIYMRVSDEPE
                     REIYPPVPYLEGVRRVVEHNGLRRVIGEVPGPTLRPSRLSVEVRQDHNLAFLRVEEPG
                     ADLEELVYKHLRDLSLHRVDCVYVDLPLSDPVTRGSAAGLEKLGVFFGGVIPEAHPVG
                     GDVLRLQYLNNVEIEAGDVSTASDFGEELLGLIFRQHASVS"
     gene            5043..5267
                     /locus_tag="VN178_06770"
     CDS             5043..5267
                     /locus_tag="VN178_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80712.1"
                     /translation="MMYSDWQYVLVQDRAAREQDLAEKMAERGRYAWSPGKPSLRARV
                     ARRLFALAVAAESEETWRIVWERLEARGRL"
     gene            5312..6103
                     /locus_tag="VN178_06775"
     CDS             5312..6103
                     /locus_tag="VN178_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS80713.1"
                     /translation="MKALELRGVEKSFKKRRGEVVRALDSVSLSVGKGEVVGILGPNG
                     SGKSTLVRVISTLVLPDRGEVEVFGVDATRHPKQVQRSMNRVSVEASFFKKLSAMENL
                     LYGAKLYGVTSAEAHPRIKEILDNIGFASKRATEPMEHLSRGMQQKIALARALLTSPV
                     VMLLDEPTTGLDPRSKKDVQEFIRKIRDAHDSSILLCTHDMGEAEELCDRVGIMMDGK
                     ILALDESEELKRRYAGEANGHLPTMEETFMAATGYSVEEASAEGD"
     gene            6105..6947
                     /locus_tag="VN178_06780"
     CDS             6105..6947
                     /locus_tag="VN178_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80714.1"
                     /translation="MHNTLKPRWLVEVIAVWGYVQRNYFLTKRYFMWELVWLTFTTVN
                     AMSITFIGAGVEMVGGGQGTDTERLMTFLLVGALIWSYLSMIFDVLSETVQWERWEGT
                     IEYTFMSPTSRVTHLLGMGVYAVAYGIARTAVVLGVAMLAFDLDLSQANYWAALVVLA
                     VCSISLVGFGVVAAVMPLLSPEKGQQVTYIVSALLLLVSGVYYPVKVLPDWMQAIAQY
                     SPVTYALEGSRAALQDGVGLSQLWGSIWPLLVMGVVFVPMGLFVFHLGESYAKRTGKL
                     KRSG"
     gene            complement(7009..7206)
                     /locus_tag="VN178_06785"
     CDS             complement(7009..7206)
                     /locus_tag="VN178_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HWS80715.1"
                     /translation="MPDSPLVRPFRYAWEYLREISGENAYDRYLSVHAATHPGKPAMS
                     RGEFYRWRQEEKYANPGSRCP"
     gene            complement(7213..9345)
                     /locus_tag="VN178_06790"
     CDS             complement(7213..9345)
                     /locus_tag="VN178_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HWS80716.1"
                     /translation="MNNSLRRYGIWVVVALVGAVCWGMLALSRGETINAGWLLFAALA
                     SYAIAYRFYARFIRDRVLGADDSRATPAERLNNGRDFEPMDRRVLWGHHFAAIAGAGP
                     LVGPVLAAQMGYLPGTIWIIVGVILAGAVQDMTTLFFSMRRDGKSLGQMAREEIGPVG
                     GAAALIAVLSIMVILLAVLALVVVNALAASPWGAFSLAMTIPIALLMGVYLRFLRPGR
                     VLEVSIIGVGLLILAIIGGGWISESSPYAAAWTFTPNQLTFALIVYGFVASVLPVWVL
                     LAPRDYLSTFMKIGTIALLAVGIAITLPPMQMPRFTEFAFNGQGPVFAGSLFPFVFII
                     IACGALSGFHSLVASGTTPKLIQKESQVRPIGYGAMLTESFVAVMAMIAAVTLDPGVY
                     FAMNANPALLGDTVQSASQAVNGFGFSVSPETLQGTADAVGEDSIVGRTGGAPTLAVG
                     ISAIFAGVTGIFGAALQAFWYHFAIMFEALFILTTVDAGTRVGRFMIQDLIGNVWRPF
                     ARPSWLPGNIIASALIVAGWGYFLWAGVNDPLGGINQLFPLFGIANQLLAAVALTVGT
                     TILIKMGKLRYAWVTALPLAWDAAVTLTASWQKIFSTDPAIGFFAQRAAAAQSIQAGE
                     LNDTMAALGAASVEDARQILVNTTVDGVLSIVFALAIIIVIADASRVWFGIIRGSKEP
                     DLSEAPYEESRLDSEGNEIEREPVGAGR"
     gene            complement(9461..10183)
                     /locus_tag="VN178_06795"
     CDS             complement(9461..10183)
                     /locus_tag="VN178_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80717.1"
                     /translation="MRFDLPDAYWKKDVALFFGRRFALVLKFLYPLALIVPVAASGIP
                     AQYAAAVIGIVAIMAGTFGAGETLTSDLNDGILERVALTPLSSRKLVFQILAVNAVLD
                     FIQLSPALLIVYVLNPAPPAWVLAATFSVFATLVAANSIGVFIANFTSSPADVLLYAT
                     AILFPLIYVSGFFRNQDPDGVPAFLRDFVPFAYMNDALKTVFGLATETTILAVVVFPT
                     IICALLVLAVCLTAPRLLSARP"
     gene            complement(10173..11135)
                     /locus_tag="VN178_06800"
     CDS             complement(10173..11135)
                     /locus_tag="VN178_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS80718.1"
                     /translation="MSSESENSPPALEAKSLSRLYKSSGRGVSDVSLRVERGEVFGLM
                     GPNGSGKSTLLRVLSTAIAPDSGSFLVGGVDGLKEKREVRTRMGLMVDRPTHYDDLTG
                     RANAYFFARAYGMEEAKAEGALAELFEYFDLAEYADDRVKTYSYGMGKKLALIEALAH
                     GPELLLLDEPSLGLDYTSQLAYQRRIRDLAGSGVAVLLASNQVDEVEALCDRAAFLHR
                     GEVVAQGSPEDLISRVEGVSRIVATLRNPVEYGSIAEVVGVQRVVPEGRDLIFHCEER
                     PGMVAEVVVGIVRSGGDIVNLSVQRPNLEDVFVELTGEALRDEV"
     gene            11211..12122
                     /locus_tag="VN178_06805"
     CDS             11211..12122
                     /locus_tag="VN178_06805"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS80719.1"
                     /translation="MVSTDDSQTGGRMADDTTVAVLGTGIMGAAMARNLLAAGIKVRA
                     WNRSREKAEPLERDGAEVVDDPRDAARGAHFVLTMLADADAAEEVVGGDVLFALAEDG
                     VWLQMSTVGEEGSGRLAEIASEHGIHYVDAPVLGTKQPAEQGQLIVLASGPEEVRERS
                     EEIFGAVGSKTVWLGEAGEGSRLKLVVNNWIVGLLGVLAETVAFAQAIGVDPVKFLET
                     IEGGPLGLPYARLKGSMMVEDDFPTSFSANLARKDAALVLDAAEAHELRMQITESVMA
                     RFDEAIQAGYDEEDMAAVYRAARSDRG"
     gene            12177..12365
                     /locus_tag="VN178_06810"
     CDS             12177..12365
                     /locus_tag="VN178_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HWS80720.1"
                     /translation="MPETKEITWDPEADRRIKRSPFLMRRFIRKRIEDRVASRGRTHV
                     TEADVDESANMYRQYRFK"
     gene            complement(12882..13634)
                     /locus_tag="VN178_06815"
     CDS             complement(12882..13634)
                     /locus_tag="VN178_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWS80721.1"
                     /translation="MNERPTLLLEDVYKSFGEDEVLKGIDLTVEPHEVVCLIGASGSG
                     KSTLLRCVNLVEPIDSGRVVVDGEEITAPGVDTNRIRRGIGIVYQSLNLFPHMTVLRN
                     ITLAPREALKTGRAEAEKNARTLLERFGLLDKQDEYPDRLSGGQQQRVAIVRALAMHP
                     HLMLLDEVTSALDPELVAEVLAVIKELAREGMTMLIATHEMSFARDIADRVCFLEAGR
                     ILEQGKPERIFTAPEHPRTRQFLGRIIEAGRL"
     gene            complement(13631..14515)
                     /locus_tag="VN178_06820"
     CDS             complement(13631..14515)
                     /locus_tag="VN178_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWS80722.1"
                     /translation="MTQGGPSAPGPSAPRNPGRGSRALGNRGVLVSSASTVLFFAIIA
                     VVVVLAPGSGVVAERFFSPQHLWQSLVGSEGLPSVLRAFWLNVQIFMVSEVFILILAL
                     VIAVVRGIPGPVFFPFRLVAVAYTDLFRGVPLILVLYMIGFGVPGLDLGFISYLPDVA
                     YGIISLVLVYSAYVAEVYRAGIESVHESQNAAARSLGLTRWQSLRFVVLPQAIRRVIP
                     PLLNDFIGLQKDTALVSVLGSIEAARAAQIYGASEFNYSSYVVAAFLFVLITIPLARF
                     TDRLIARDKRRREAGALA"
     gene            complement(14517..15341)
                     /locus_tag="VN178_06825"
     CDS             complement(14517..15341)
                     /locus_tag="VN178_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWS80723.1"
                     /translation="MRERVTLIGLLFVVLLLVGCGRPSAESGPTDKCELANPPLFEKG
                     QLTVATDKPAYPPWFTGNPKDYSGYEGDVASEIANRMDLPIKWTVEPFNKSYAPGSKD
                     FDFDINQITITKEREQAVDFSEGYFDNAQGILVMKDSPAASAQSVSALKDLTIGAQVG
                     TTALDFVNETVKPTEEPKVYDSTNDAKSALESGQIDVFVTDLVTTVYLRDFEIDGSTV
                     IGKYPRNEQFGMLFEQGNPLVGCVNEVLGEMKSDGTLEKLQKKHLQQYLDVPTLKK"
     gene            15563..16342
                     /locus_tag="VN178_06830"
     CDS             15563..16342
                     /locus_tag="VN178_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HWS80724.1"
                     /translation="MSESGQQRYESPLLSDRGATTISGTVVSTIAGMAAQEVDGVHMG
                     GGASRTASGVLGSLTGSESSTSGISVEVGRTEAAIDLKMGIEYNKNILQTVEEVRRRI
                     TDRVQSMTGLRIKELNATITDITFPEKEERRRIGSRSETTEPDASADESAIAPTSPAT
                     PDSEPEPAASEHDTTRVETRRQPESVTTPETDAGAEDRPEVTSELRSGDAGTEEPSTV
                     ETPEEAQTRAETETPGPQETRAEARRRRRAEERARRRTEDS"
     gene            16393..16764
                     /locus_tag="VN178_06835"
     CDS             16393..16764
                     /locus_tag="VN178_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HWS80725.1"
                     /translation="MDQTTYSPRITQLSWGRLETESGVFKDVKLYPGGVEEWDWNETG
                     TSHDPGIQPADVERLLQRGSTVVILSRGFHERLGVAPETLEMLEEREIPAHVLQTEEA
                     ARLYEELRETEQVGALIHSTC"
     gene            complement(16854..17549)
                     /locus_tag="VN178_06840"
     CDS             complement(16854..17549)
                     /locus_tag="VN178_06840"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HWS80726.1"
                     /translation="MRTPPVVSPEEWKTAREELLVKEKEHTRARDALAAERRRMPWMA
                     VEKEYSFEGPNGPATLADLFDGRRQLIVYRAFYGPEVTTYANPERGDAYPERACAGCS
                     FLADQIAHPAHLNARDVTLAFASRAPQADLRKLKERHGWEHIPWYTITDDWDKDFGVD
                     EWHGHNAFIREGNQIYRTYFIDSRGDEAMGTTWSYLDMAPLGRQENWEDSPEDYPQTA
                     PYGWWDYNDAYGT"
     gene            complement(17896..18306)
                     /locus_tag="VN178_06845"
     CDS             complement(17896..18306)
                     /locus_tag="VN178_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWS80727.1"
                     /translation="MEGSRALEVRLYEGLRAAGYGDVRPAHYAVFRYLEKGGSRVTEL
                     AEAAGMTKQSMGELVSYLEGRGYVTRRPDPRDGRARIVSLTEAGRRGIEAAAGCLAEI
                     EADLAERMGEEELERLSGLLGDLAAFLDEPGTTR"
     gene            18434..19054
                     /locus_tag="VN178_06850"
     CDS             18434..19054
                     /locus_tag="VN178_06850"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS80728.1"
                     /translation="MMEEAGYSHSGAQSPGMVSGGDGELVHRDGHRVVFLEEGMDGRG
                     AYLMIEHVWTRPGMMAGPHWHPVLQETFSVREGRMRFRVDEREFELGPGERVTVRPGE
                     VHRFWNEGEGRLTAIHEVRPPGHHREMFELWDRLDLAGKTNRQGVPKDPLALGLLWEL
                     QDGYLAGVPALLQRLVLGSLARLARVVGYQERLIGSSSRKDERVRA"
     gene            19051..>19392
                     /locus_tag="VN178_06855"
     CDS             19051..>19392
                     /locus_tag="VN178_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80729.1"
                     /translation="MSAGQTRTHAKLAGRRGPAALVDWQSALYAFVLLLGCVFLVPLT
                     KFWWVVLLLGVAAPIALAVLSGGTTRVEKPGFGRESEEELLALAERGSLTPTTAAMRT
                     SLTVEQASKMLD"
BASE COUNT         3772 a         5918 c         6295 g         3407 t
ORIGIN      
        1 tgctgtgccg atgcccctac cctgccagtc atcccgcact gctataccga agtgtcccga
       61 gtgtcgggtc cttgggttcg tgtaggcgct gagtgccaga tggccgacga cctcaccgtt
      121 tacgcaggct accagggcga agctatcgtc tcgttccctg accagctctt cgcgccatgc
      181 ttgctccgaa gagtacggaa ctccgagcgt ttcggccatc gcccgcgggg aggagtagac
      241 gaggtgcacg gctttgtaat cttccggttc gcagcgccgg atgaccaggt cacgcatgcc
      301 gactctcctc ctttcctgac cgtgtatttt cgcatggact tccgaaactc ggattctcac
      361 acaaaacctg actagctgaa gtgctgacag actgaaatgc tggcaagggc gattgtgtaa
      421 taaactcggg cggtggtatc agagaactcc acgatagagg agggatccgt ggatcagcgc
      481 aatctgggtg gcgaagggct cgtcgtatcg gagctcggac ttgggtgcat ggggatgagc
      541 gagttctacg gcgcggggga cgaagaggag tccacggcga ccatccaccg cgcgatagaa
      601 ctgggcatca ccttcctgga cacggcggac atgtacggcc cgttcaccaa cgagaggctc
      661 gtcggcaaag ccatagccgg ccgcagggac gaggtcgtgc tcgccaccaa gttcggcaac
      721 gtgcgcggcg aagacgggag cttccacggc gtaagcggga aacccgacta cgtgagagag
      781 gcctgcgacg cttcgctttc gcgcctcggg gtcgaccaca tagacctcta ctaccagcat
      841 cgcgtggacc cggagacacc catcgacgag acggtcggcg cgatgaagga gctcgtggag
      901 gccggcaaga tcaggtacct cgggctctcc gaggcggggg cggagaccat caggaaggcc
      961 cacgccgtgc accccatcag cgccctccag tcggagtatt cgctctttac ccgcgacgtg
     1021 gaggacgaga tcctacccac cgtccgagaa ctgggcatcg ggttcgtccc ctacagccct
     1081 ctcgggcgag gcttcctcac cggacgctgg aggagcatcg aggacatgcc cgaagacgac
     1141 acccgcagcg cccgcttccc acgattcgcc gaggagaact tcaagaagaa tctggagctg
     1201 gcggataggg tgcgcgaggt cgccgaaggc aaggggatca ccccggggca actggccctc
     1261 gcgtggctgc tcgcccaggg agatgacatc gtccctatcc cggggaccaa gcgccgcgag
     1321 tacctcgaag agaacgctgc cggagccggt gtaacgctca ccgaggacga cctcgccggc
     1381 atcgaggagg ccatgcctcg cggatcagcc gccggcgagc gctacgccga agagcagatg
     1441 agggccgtag gccgctagga ggagccgatg accgatgtcg agcatatctc gcgcaagggg
     1501 acgggtgtgc ccgtaagctg gttccccatc cccgaagagt ccgagcttcc cgatgagctg
     1561 caggggcttt tcaggaaggc cagggagatg gtcgggttcg tccccaacgt cttcagggct
     1621 tacagctacc gtccagagag gctctccgcc tggttcgccc actaccgcca gctacacgag
     1681 ccgaccccga acctggacgc ggcggagagg gagatgatag ccgtggccgt ctccatggcg
     1741 aacggctgcc tctactgcct ggtcgcccac ggcgccgccc ttcgccaggc cctgggcgac
     1801 cccatactcg ccgaccggat caccctggat cacaggaggg ccggcctcga cgagcgccgc
     1861 acggccattc tggatttcgc cgtcaagatt accgagcgtc cgctggattg tgatccgaaa
     1921 gacctcgaac gcctcagggg ctacgggctg accgaggagg aagtctggga cgtgatcgag
     1981 atcgcaagca tgtataactt caccaaccgc atgtcgctgg cgtgcggcat gatccccaac
     2041 gaggagtacc acgccctggc ccgctagcgg gccgtccgtg gaggtgcccg tgctgcaggt
     2101 gatctcggtg ttcggttcgc tgtctatact ggctgccttc gttgccaacc agcgagggtg
     2161 gatctcccca tcccagcttt cttatgcggc cgccaacttc gtcggggcgg tgatcctgac
     2221 ggtggtcgcc atagtggacc agcaggtcgg gttcatactg ctccagggca cgtggaccct
     2281 ggtgagcctg atagggatag tcaggatatt gcgcggagac gagtcccgcc gcgtccggta
     2341 gggaggacga cgatgttgca ggcggtatcg gtagtcgggg cgctgctcat actgttggct
     2401 tatgcggcca accagttcgg ctggaccgac acctcgagct tacgctacca gatcgccaac
     2461 ttcgccggct ccacgattct gaccgttgta gccgtgatcg aggtgcagat cggctttatc
     2521 ctcctggaag gggtgtgggc cttggtcagc gcgtggggga tcgtctcggt actacgcggc
     2581 ggcagatccg gcgacacaaa ctagctcccg accgggaagt catggtctgt atactggtgc
     2641 ctggagcgct agagaggaga agggtgtgtc aggagtaaag tacgtctcgg aggtcaaggt
     2701 ggagccggtg gagggtaaga tccgccgcgc gtatctcccc gtccaggacg atcctgtggt
     2761 gttcggcgtc cacagcgagg tcgccgaaca ctacggcgtc ccccctgagg aggtcgaacc
     2821 gtacgacacc acgctcgact acgtggtcgc cgccgcgggc ggctgactgc tcggcacctt
     2881 ttcgggcgcg ctggcagcgc gtcaggtgtc gttcgataac ctccacgccc agacggtcgg
     2941 agagatcgag gtcgaacgga aagtgctcgt catcaagcgc atcaaacaga ccttccacct
     3001 taccgccgcc gaaagcgacc gcgagaccat agaaagggtg ctcgaagtct acgcggatag
     3061 ctgtccggtg gcggcttccg taaaggggag catcgagatc tccagcgagt tggacttcac
     3121 ggccgcctga ggtgcctgct agctcacgga agcgtgctgg cggaagatca aaccgaggag
     3181 ctcttctccg aagtccgagg ccgtgctgac gtcgccggct tcgatctcga cgttgttcag
     3241 gtactggaga cggagcacgt ctcctcctac ggggtgggct tcggggatga ccccgccgaa
     3301 gaatacccct agcttctcca gcccggccgc ggagccccgc gtcacgggat cggagagcgg
     3361 aaggtccaca tagacgcaat ccacgcggtg caggctgaga tctcgcaggt gtttgtagac
     3421 gagctcctcg aggtcggccc ctggctcctc gacccgcagg aaggcgaggt tgtggtcctg
     3481 acgtacctcg acgctcagac gggagggccg tagcgtcggg ccgggtacct cgccgatcac
     3541 ccgtcgcagc ccgttgtgtt cgacgacgcg cctgacccct tccagataag gcacgggagg
     3601 gtagatctcc cgctccggtt cgtcactgac gcgcatgtag atcagggcga cggacccgcg
     3661 acgatccgcc ctctcatcac cgatctgctt gtacgagacg gaggctggga tgtagccgag
     3721 caagaagcct gtctccctcg ctccgagatg caggttgccc ctctggctgt acggatgcac
     3781 ggtggtcgcc tcgctgtaga gcccgtacat gccggcgtca gcggcccacc gtgccataaa
     3841 ggtcttcatc ttcgggaaca ggtggtgacc gcgaaaccgc gggtccacga ccgcctgccc
     3901 ggattcacca actggcgagc cgggacgttc cacggtaatg gccagatgcc ccacgaactc
     3961 gtcttcgggc gttaccgcga cgcaggagac catgaggccc tgctcctgaa gctccctgat
     4021 cctgtcggga aagtagatgt agtcccagtc gtagctgtac ccgtaggagc ggtagacgca
     4081 gcgcgagagc tcgaaggatt cctcaggatg catcatgcga atctccagag ggatatcctc
     4141 gtgcgcggcg gtcgcttcca cggttctgtg gtgctcttct tctggtaggt gatccctcac
     4201 gtctgtgtga gggaggtgct tgaggagctc gaccctgttg ccgcttcggc cgaggtttac
     4261 gaagcgggtc tcgtcgaagg atctgtggag tgtctcgagc acggtccgat cctccccgtc
     4321 ctgcaggcgt ttgtagtcga aaggcagccc ctggtcctcg acggccacga ccacgtgccc
     4381 cggtcggcgc aggatctcca agtccaagaa ccctttttct tcggacccga aggcgttctc
     4441 gatgacgttc aggcagacca gttcgacggc ccggtctaca ggctctacgt cccccaggcc
     4501 gagccgccgg gtcatctgct tcacgaactc gatcacgggc ggcaagaacc cggcgtctgc
     4561 ggagatcgtg aggcacgcga tcggttcccc tggcgtttgt ttgagctcgg tcacaagaga
     4621 tccttctcga tattcaccat tcgcatcagt accctattct ctcaggtccc gttggcgtcg
     4681 atcaaacaat cggcacccac aaaaagctga ctagctgtaa aaacgttgac aagttctggg
     4741 tggcagagta gagttctaac gatgttgttg tcgcgaaaag gactgaagga ttgatgcaca
     4801 tccgtcgcac gaacataggt aatccggtgt tcgggggaga ggtgcgtccc gaggtccgcg
     4861 aggtcgcgat gagaacgtga gcgtctagct caaccacact tctcaggaga ggcccccgga
     4921 ccgagtggac cgggggccta aagctttgct ccgagagagc tggcggcggt cgcaggctcc
     4981 tatcccaaca cggacggtat cagcccttcc aaggcgcgac gaggagacga gagaaggaga
     5041 aagtgatgta cagcgactgg cagtacgtgt tggtacagga ccgggcagcc cgtgagcaag
     5101 acctcgccga aaagatggcc gagcgcggga gatacgcgtg gtctccggga aagccctccc
     5161 tgagggccag ggtggcgcgc aggctcttcg cgttggcggt agccgccgag agcgaggaga
     5221 cctggcgcat cgtctgggag aggttggagg ccagggggcg tctgtagagt tgggcgcccc
     5281 tcccggcaag gttcctatag gaggtgcggt tatgaaggca ttggagttaa gaggagtaga
     5341 gaagagtttc aagaagcggc gtggggaggt ggtacgcgcc ctcgactcgg tctcgctctc
     5401 ggttggcaaa ggggaggtcg tcggaattct cgggcccaac gggagcggga agagtacgct
     5461 ggtgcgggtt atctccacgc tcgtcctccc cgacaggggt gaggtcgagg tcttcggggt
     5521 cgacgccacg cgccatccca agcaggtgca gcgcagcatg aaccgggtca gcgtcgaggc
     5581 aagcttcttc aagaagctct cggccatgga gaatctgctc tacggggcga agctctacgg
     5641 cgtcaccagc gccgaggcgc atcccaggat aaaggagatc ctcgacaaca tcggcttcgc
     5701 cagcaagcgg gccaccgagc cgatggagca cctgagccga gggatgcagc agaagatcgc
     5761 tctagccagg gcgttgctca ccagccccgt cgtgatgctc ctcgacgagc cgactacggg
     5821 tctcgacccc aggagcaaga aggacgtgca ggagttcatc cgcaagatac gcgacgccca
     5881 cgactcctcg atcttgctct gcacccacga catgggcgag gccgaggagc tctgcgaccg
     5941 cgtcggcatc atgatggacg ggaagatact ggccctcgat gagtcagaag agctcaaacg
     6001 ccgctacgcc ggcgaagcca acgggcactt gcccaccatg gaggagacgt ttatggctgc
     6061 gacaggttac tccgtcgaag aggccagtgc agaaggagac tagtatgcac aacacgctca
     6121 agccccgatg gctggtcgag gtaatcgccg tctggggcta cgtacaacgc aactacttcc
     6181 tcaccaagcg ctactttatg tgggagctgg tgtggctgac gttcaccacc gtcaacgcca
     6241 tgtccatcac ctttataggg gccggtgtcg agatggtcgg cggcggtcaa gggacggata
     6301 ccgagcgtct gatgaccttc ctgctcgtcg gcgcgctgat ctggagttac ctctccatga
     6361 tcttcgacgt gctctccgag acggtgcagt gggagcggtg ggagggaacg atagagtaca
     6421 cgttcatgag cccgacgagc cgggtcaccc accttctcgg catgggcgtc tacgctgtcg
     6481 cctacggcat cgcccgcacc gcagtggtcc tcggcgtcgc catgctcgcc ttcgacctcg
     6541 acctctcgca ggccaactac tgggccgccc tggtcgtcct cgccgtgtgc agcatctcgc
     6601 tcgtcggctt cggggtggtg gccgcagtga tgccgctgct ctcgccggag aaaggccagc
     6661 aggtgacgta tatagtctcg gccctcctgc tcctcgtctc gggcgtctac taccccgtca
     6721 aggtgctccc cgattggatg caggcgatag cgcagtactc accggtcacg tacgcgctcg
     6781 aaggatcccg cgcggcgctc caggacggag tcggcctctc acaactgtgg ggtagcatct
     6841 ggccgctcct cgtcatgggc gtagtcttcg taccgatggg gctcttcgtc ttccacctcg
     6901 gcgagagcta cgccaagcgg accggaaagc tcaagaggag cgggtaaaga ggtttcatgt
     6961 aaggccctat tcaatcgcct tgccggccga ccggccggca gggcttcatc agggacatcg
     7021 gctgcctgga ttggcgtact tctcctcctg gcgccagcgg tagaactcgc cgcggctcat
     7081 ggccggcttg ccgggatggg tcgcggcgtg gaccgacaga taacggtcgt aggcgttctc
     7141 gccggagatc tccctcaggt actcccaggc gtagcgaaag ggtctcacga gtgggctgtc
     7201 tggcatattc tcctagcggc cggctccaac tggttcgcgt tcgatctcgt tgccttccga
     7261 gtcgaggcgt gactcctcgt agggcgcttc gctcaaatct ggctccttgc tcccgcggat
     7321 gataccgaac catacgcggg aagcatccgc tatcacgatg atgatggcga gcgcgaagac
     7381 gatcgagagc actccgtcca ccgttgtgtt cacgaggatc tgacgcgcgt cttctacgct
     7441 cgcggcgccc agcgcggcca ttgtgtcatt cagttcgccg gcctgtatgg actgggcggc
     7501 tgcggcccgc tgggcgaaga agccgatggc tgggtcggtg gagaagatct tctgccagct
     7561 cgccgtgagc gtcaccgccg cgtcccacgc cagaggcaat gccgttaccc aggcgtacct
     7621 cagcttgccc atctttatga ggatggtcgt cccgaccgtc agggcgacgg ccgccaggag
     7681 ctggtttgcg atgccgaaga gggggaagag ctggttgatg ccgcccagag gatcgtttac
     7741 gccggcccag aggaagtagc cccagccggc gactatgagg gcgctggcta tgatgttacc
     7801 gggcagccag gaggggcggg cgaagggcct ccagacgttg ccgatgaggt cctggatcat
     7861 gaaccgtccg acgcgggttc ccgcatcaac cgtagtcaag atgaacaggg cttcgaacat
     7921 gatggcgaag tggtaccaga acgcctgcag ggccgcgccg aagattccgg tgaccccggc
     7981 gaagatcgcg gatatgccga ccgcgagcgt cggcgcgccg ccggtccttc cgacgatgga
     8041 gtcctcgccg acggcgtctg ccgtaccctg gagcgtctcg ggacttacgg agaacccgaa
     8101 gccgttgacg gcctgggagg ccgactggac ggtatccccg aggagcgccg ggttggcgtt
     8161 catggcgaag tacacgcctg ggtctagcgt gaccgcagct atcatggcca ttacggcgac
     8221 gaacgactcc gtgagcatcg cgccgtagcc gatcggtcgc acctgcgact ctttctggat
     8281 cagcttgggc gtggtgcccg acgcgacgag cgagtggaac cccgatagcg ccccgcaggc
     8341 gataatgatg aacacgaatg ggaagagcga accggcgaag accggcccct gtccgttgaa
     8401 ggcgaactcc gtgaagcggg gcatctgcat cgggggcaag gttatggcta tgcccacggc
     8461 gagcaaggct atcgtcccga tcttcatgaa cgtcgagagg taatcacgtg gtgccagaag
     8521 gacccacaca ggcaggaccg aggccacgaa accgtagact atgagggcga aagtgagctg
     8581 gttcggggtg aacgtccacg ccgcagcata gggagaactc tcggagatcc aacctccccc
     8641 gattatggcg agtatcagga gaccgacgcc gatgatcgag acttcgagca cccgtccggg
     8701 gcgcaggaaa cgcaggtaaa cacccatcag cagggctatg gggattgtca tagcgagcga
     8761 gaaagcgccc cacggggatg ccgcgagcgc gttgaccacg accagggcga gaacggcgag
     8821 tagtatcacc atgatcgaga gcacagctat gagcgccgct gctcctccta cgggtccgat
     8881 ctcctccctt gccatctggc cgaggctctt gccatccctg cgcatggaga agaagagcgt
     8941 cgtcatgtcc tgcacggcgc cggcgaggat gacgccgacg atgatccaga tcgttccggg
     9001 caagtagccc atctgggccg ccagaaccgg accgacgagc ggtcccgccc cggctatcgc
     9061 cgcgaagtgg tgaccccaga gcacccgccg gtccatcggc tcgaagtccc ggccgttgtt
     9121 gagcctctcc gcaggggtgg ccctagagtc gtccgctccc agtaccctgt ccctgataaa
     9181 tcgcgcgtag aagcggtagg caatggcata agatgccagt gctgcgaaca gcagccaccc
     9241 ggcgtttatg gtctcaccgc gggagagggc cagcattccc cagcagaccg ccccgacgag
     9301 agcgacgacg acccagatcc cgtaccgacg aagactatta ttcatgattc cctccgactt
     9361 tcccgacgca tattcatttc atcatagcgg aaaaccgtat cgaatgcggg ggcctatggt
     9421 ccgagtagag aacatcgctt catattacat cgtgaccgcg tcacggacgc gcggagagga
     9481 gccgcggtgc cgtcaggcac accgctaaca ccaggagggc gcagatgatc gtgggaaata
     9541 cgacaactgc gagtatagtg gtctccgtag ccagaccgaa taccgtcttc agggcgtcgt
     9601 tcatgtaggc gaaaggaacg aagtcccgca gaaaggcggg gacaccgtca gggtcctggt
     9661 tgcggaagaa tcccgagacg tagatcaggg ggaagaggat cgccgtcgcg tagagcagca
     9721 cgtccgcagg cgaagatgtg aagttcgcta taaacacccc gatgctgttc gccgcgacga
     9781 gcgtcgcgaa cacggagaag gtggccgcaa gcacccacgc gggcggcgcg gggttgagaa
     9841 cgtagactat caagagcgcg ggcgagagct ggatgaagtc caggaccgcg ttcacggcca
     9901 gtatctggaa gaccagtttt cgtgacgaca ggggggtgag ggccacgcgc tccaggatgc
     9961 cgtcgttcag gtcgcttgtg agggtctctc cggcgccgaa cgtgccggcc attatcgcca
    10021 cgatacctat gacggccgcg gcgtactgcg ccggtattcc gctcgccgca acggggacga
    10081 tcagggcgag cgggtagagg aacttcagga ccagggcgaa cctccgcccg aagaacagcg
    10141 cgacgtcctt cttccagtat gcgtcgggga gatcaaacct catccctgag cgcctccccg
    10201 gtgagctcta cgaacacgtc ctccaggttt ggcctctgca cagagaggtt cacgatgtcc
    10261 ccgccagagc ggacgatgcc gaccacgact tcggcgacca tgcccggacg ttcctcacag
    10321 tggaagatga gatcccttcc ctccggaacg acgcgctgta caccgacgac ctcggctatg
    10381 gagccgtact ccacgggatt acgcaacgta gccacgatac gactgacacc ttcgacgcgg
    10441 gagatgagat cttccggcga tccctgggcg acgacctctc cgcggtgcag aaaggccgcc
    10501 ctgtcgcaca gagcctcgac ctcgtcgacc tggttgctcg cgagcaggac cgccacaccc
    10561 gagccggcga ggtccctgat cctgcgctgg taggcgagct gcgaggtgta gtcgagcccg
    10621 agcgaaggct catcgagcag tagcaactca ggcccgtgcg ccaacgcctc gatcagggcg
    10681 agcttcttgc ccatcccgta cgaatacgtc tttaccctgt catccgcata ctccgcaaga
    10741 tcaaagtact cgaagagctc agcgagcgcg ccctcggcct tggcctcctc catcccgtag
    10801 gcgcgggcaa aaaagtacgc gttcgcccgg cccgtgaggt cgtcgtagtg cgtcggacgg
    10861 tccaccataa gacccatgcg ggtccggacc tcccgcttct ctttcagacc gtctaccccg
    10921 cctaccaaga agctgcccga gtcaggcgcg atggcggtag acagaacccg caaaagggtg
    10981 ctcttacccg agccattcgg ccccatgaga ccgaagacct caccgcgctc cacgcgcaaa
    11041 cttacgtcag agactccgcg cccgctgctc ttgtagaggc ggcttaagct cttcgcctcc
    11101 agggccggtg gggagttctc tgattccgag ctcatggcac gagaattgta tcgccctccc
    11161 agcaggacgg ttgtcagacg catcaccccg tgtttgcgcc gtcgtagggt atggtaagta
    11221 cggacgactc gcagacagga ggacgaatgg ccgacgatac gacggtggcg gttctgggga
    11281 cgggaatcat gggcgccgcg atggctcgta atctcctcgc tgccggcata aaggttcggg
    11341 cgtggaaccg ctcgcgcgag aaggctgagc cgttggagag ggatggcgcg gaagtagtcg
    11401 acgatcccag ggatgccgca agaggcgcgc acttcgtcct gaccatgctc gctgacgcag
    11461 acgccgcaga ggaagtggtc ggaggagatg tgctcttcgc gctggccgaa gacggggtat
    11521 ggttgcagat gagcacggtg ggggaagagg gaagcgggcg gctcgcggag atagccagcg
    11581 agcacggtat ccattacgta gacgccccgg ttcttgggac aaaacagccc gcggagcagg
    11641 gccagctcat agtgctcgcc tcggggcccg aggaggtcag ggagcggagc gaggagatct
    11701 tcggtgccgt gggaagcaag accgtgtggc tcggcgaggc tggtgaagga agtcgcctca
    11761 agctcgtcgt caacaactgg atcgtgggtc tactcggtgt gctcgccgag accgtcgctt
    11821 tcgcacaagc aatcggagtg gaccccgtga agtttctcga gaccatcgag ggcggaccgc
    11881 tgggcctacc atacgcccgg ctgaaaggca gcatgatggt cgaagacgac ttcccgacca
    11941 gcttctcggc caacctcgcc cgcaaggacg cggccctcgt cctcgacgcc gcagaagccc
    12001 acgagctgcg aatgcagatc accgagtccg ttatggcccg cttcgacgag gcaatacagg
    12061 caggatacga cgaagaagac atggcggccg tctacagggc cgcaagatca gaccgcggct
    12121 gacagagcca ggagcgtggg aatataatcc ctccgcaacc tcagaggaga atgtgcatgc
    12181 ctgagactaa agagatcacc tgggaccccg aggcagatcg caggataaag cgcagcccgt
    12241 tcctgatgcg ccgttttatc cgcaagcgca tcgaggatag ggtggcttcg aggggccgta
    12301 cccacgtcac cgaagccgat gtggacgaga gcgcaaatat gtaccgccag tatcgattta
    12361 aatagctgtc agctatcagc ggtcagccgt cagccctttg atttgtggcc tggcgtactc
    12421 tcaaaaacga tcccggaaga ctcgcgcagg taacaagtgt ttcgcagaag cctttgcttc
    12481 atgcaagaaa gataagctga aagctgatgg ctgaaagctg accgctccca aaaatatccg
    12541 ggccggagag ccgctctttt ggcgaacgac caccgacccg gtccgcggac ctgtaggggc
    12601 ccgcagatgg ttgttgacta gctagtccac tggacccttt cctcctagtc gaggtgcaac
    12661 taccagggcc atggtaccat ctctcgcgag ccccgcgcta ctactttcgg accatttcgt
    12721 gcaccccata cgaaagcact accacatgcc tttgggcccg cgacaccttt cgcatcggtt
    12781 ggcccaggaa ggtaatacac ttcgcgcccc ggactccatc gggatccaca gaagaaacct
    12841 gatgcctgaa acctgatgcc tgaaacctga tgcctgaaac cttaaagccg gccggcctcg
    12901 attatccgtc cgaggaactg gcgggtgcgg gggtgttcgg gagctgtgaa gatcctctcg
    12961 ggcttaccct gttcgaggat tcggccggct tcgagaaagc agacgcggtc tgctatgtcg
    13021 cgggcgaaac tcatctcgtg ggtcgcgatt agcatggtca tcccttcacg ggccagctcc
    13081 ttgatgacgg cgaggacctc ggcgaccagt tcggggtcga gggcgctcgt gacctcgtct
    13141 aggagcatga ggtgagggtg catcgcgagc gccctcacta tggcgacccg ctgctgctgg
    13201 ccgccggaga gcctgtcggg atactcgtct tgcttgtcta ggagcccgaa acgttccagg
    13261 agcgtgcggg cgttcttctc ggcctcggcg cggcccgtct ttagggcctc gcgaggggcg
    13321 agggtgatgt tgcgcaggac cgtcatgtgt ggaaaaaggt tgagggactg gtagacgatg
    13381 cctatgccgc gccggatacg gttggtgtcg acgccggggg cggtgatctc ctccccgtct
    13441 acgacgaccc tgccggaatc tatcggctcg accaggttga cgcacctgag tagcgtggac
    13501 ttgcccgagc ccgaagcccc gatcaggcat accacctcgt gcggctctac cgtcaggtct
    13561 atacccttca gaacctcgtc ctccccgaag gacttgtaga catcttccag gaggagggtg
    13621 gggcgttcgt tcatgccagg gctccagcct cgcggcggcg tttgtcgcgg gcgatcaggc
    13681 ggtccgtaaa gcgggcgagg gggatggtga tcaggacgaa aaggaaggcg gcgaccacgt
    13741 agctcgagta gttgaactcc gaggcgccgt agatctgggc cgcacgcgcc gcctcgatag
    13801 agccgagcac cgagacgagc gccgtgtctt tctgcaaccc tataaagtca ttgaggagcg
    13861 gtgggatcac ccgccttatg gcctgcggca acaccacgaa cctcagggac tgccagcgcg
    13921 tgagacccag agagcgggcc gccgcgttct ggctctcgtg cacggactcg atgccggccc
    13981 ggtagacctc ggcgacgtag gccgagtaga cgaggaccaa tgagataata ccgtaggcca
    14041 cgtcggggag gtaggagatg aagccgaggt cgaggcctgg cacgccgaac ccgatcatgt
    14101 acaggaccag gatcagaggc acccccctga agaggtccgt gtacgcgacg gcgaccagcc
    14161 ggaaagggaa gaacacgggc cccggtattc ccctaaccac ggcaataacg agggccagga
    14221 tcaggataaa gacttcggag accatgaaga tctgcacgtt gagccagaaa gccctgagga
    14281 ccgaaggcag gccctcagag ccgactagcg attgccagag gtgttgcggg ctgaaaaacc
    14341 gctcggccac gaccccgctc cccggagcga gcaccacgac taccgcgatg atcgcgaaga
    14401 acagcacggt gctcgcgctc gataccagga ccccccggtt ccccagggcc cgcgagcccc
    14461 tacccgggtt ccgtggcgcg gacggtcctg gggcggaggg acctccctgc gtcacgctac
    14521 ttcttcaggg tgggaacgtc caggtactgc tgcaggtgct tcttctggag cttctcgagg
    14581 gtgccgtcgc tcttcatctc gccgaggacc tcgtttacgc agccgacgag cgggttgccc
    14641 tgctcgaaga gcatcccgaa ctgctcgttg cgcgggtact tcccgattac ggttgagccg
    14701 tctatctcga aatcgcgcag gtagaccgtc gtcaccagat cggtgacgaa gacgtcgatc
    14761 tggccgctct ccagggccga cttcgcgtcg ttggtggagt cgtagacctt gggttcttcg
    14821 gtcggcttta ccgtctcgtt gacgaagtcg agagccgtcg tgccgacctg ggcgcctatg
    14881 gtcaggtcct ttagtgcgga gacgctctgc gcgctggcgg cgggggaatc cttcatcacg
    14941 agtatgccct gggcgttgtc gaagtagcct tccgagaagt cgacggcctg ctcgcgctct
    15001 ttggttatgg tgatctggtt gatgtcgaag tcgaagtctt tggaaccagg ggcgtaggac
    15061 ttgttgaaag gctcgacggt ccacttgatg gggaggtcca tgcgattcgc gatctcactc
    15121 gctacgtcgc cctcgtaccc cgagtaatct ttggggtttc ccgtgaacca cggcgggtag
    15181 gccggcttgt ccgtcgcgac cgtcagctgg cctttctcga agagcggcgg gttggccagt
    15241 tcgcacttat ccgtgggacc agactccgcc gaaggtctgc cgcagcctac gagcagcaga
    15301 accacaaaga ggagcccgat cagggtaacc cgctctctca cacgcatctc cgatccactc
    15361 tccacacgaa gacagatggc cggctcgtag ggaccctgac catcatatac cccgcaggaa
    15421 gtatagaagc gccgggggag cgttgcaatc gatctcttgc catccgtcgg agaccttcgc
    15481 cgcacgaatg ctggtattct ggttgcgccg gggccgttcg gggaacactc tagagaacct
    15541 ggtcgaatga ggagagttag agatgagcga gtcagggcag cagcgctacg aaagcccgct
    15601 actgagcgat cgcggggcca cgaccataag cggcacagta gtctccacca tagccggcat
    15661 ggcggcgcag gaggtagacg gcgtccacat gggcggggga gcctcgagaa ccgccagcgg
    15721 agtactgggg agccttacgg gttcggagag cagcaccagc gggatctccg tcgaggtcgg
    15781 aaggacagag gcagcaatag acctcaagat ggggatcgaa tacaacaaga acatcctcca
    15841 gaccgtcgag gaggtgcggc gcaggatcac agaccgcgtc caaagcatga cggggctcag
    15901 gatcaaggag ctcaacgcca caataacgga catcaccttc cccgagaagg aagagcgtcg
    15961 ccgcatcggg tcgcggagcg aaacgacgga gcccgacgcc tccgcggacg agagcgcaat
    16021 cgcccccaca tcgccggcca cacctgacag cgagccggag cccgctgcca gcgagcacga
    16081 tacgacgcgg gtagagaccc gcaggcagcc cgagagcgtt accaccccag agacagacgc
    16141 aggcgcggag gatcgtccag aagtgacctc agagctgcgt tctggcgacg cggggacaga
    16201 ggaaccgtcg acggtcgaga cgcctgaaga ggcccagacg cgtgcggaga ccgaaactcc
    16261 cgggccgcag gaaacgaggg ccgaggctag gagaaggcgc cgcgctgagg agcgagcgag
    16321 gcggcgcacc gaagacagct agcaacttcg cagttagtgg gatcgaagcg aatctattgg
    16381 agggcgagca gaatggatca gacaacctac tcaccgcgga taacccagct ctcctggggc
    16441 cgcctggaga ccgagagcgg tgtcttcaag gacgtaaagc tctaccccgg aggggtggaa
    16501 gagtgggact ggaacgagac gggtacaagc cacgatcccg gcatacagcc ggccgacgtg
    16561 gagagacttc tgcaacgggg ctcgaccgtc gttatcctct ccagaggctt ccacgaacgt
    16621 ctcggcgtcg cgcccgagac actcgagatg ctggaagaaa gggagatacc cgcgcacgtc
    16681 ctacagaccg aagaggccgc ccgcctctac gaagagttgc gggagacaga gcaggtggga
    16741 gcgctgatcc actcgacctg ctgatctagc ttgacaggac acgaagcgcg atcacgcgga
    16801 atcacgtatc gcgcttcgtg tcgaacaaca tctccctgtt gagcgctccg cgctcatgtg
    16861 ccgtaggcat cgttgtagtc ccaccacccg tagggcgctg tctgcgggta gtcctcgggc
    16921 gagtcttccc agttctcctg gcggccgagc ggggccatgt ccaggtagct ccaagtggtg
    16981 cccatggctt cgtcgccgcg gctgtcaatg aaataggtgc ggtagatctg gttaccctcg
    17041 cggatgaacg cgttgtgccc gtgccactcg tcgacgccga agtctttgtc ccagtcgtcg
    17101 gtgatcgtgt accaggggat gtgctcccac ccgtggcgct ccttcaactt ccggagatcg
    17161 gcttgcggtg cgcgcgaggc gaacgcgagc gtcacatccc gcgcgttcag gtgggcgggg
    17221 tgggcgatct ggtccgctag gaaggagcag cccgcgcacg cccgctcggg gtatgcgtcg
    17281 ccacgctccg ggttcgcgta ggtggtgacc tccgggccgt agaaggcacg gtagacgatc
    17341 agctgacggc ggccatcgaa caggtcggca agtgtggccg gtccgttcgg gccctcgaag
    17401 ctgtactcct tctcgaccgc catccacggc atccggcggc gctcggccgc gagcgcgtca
    17461 cgggctcggg tatgctcctt ctccttgacg agcagttcct cgcgggcggt cttccactcc
    17521 tcgggggaga cgacgggggg tgttctcact ttgctccttc ctccttcggg tcctcgtcga
    17581 cgaggttcac gaagcggccc cagaggcccg ctggcgatcc atcgactctg cccatctcgt
    17641 ggtgcgcgtc gcgttcgaaa atgtttggtc acggccaaac ctggacgctc ctatccctgt
    17701 cgaatgtctg ttgtgaggtt catgcccaca acaactcctt gaccaggatc tctcatatca
    17761 aacttacccc acatatgacc gaaacactac tgccaatttg aaggccagct acttcttcgc
    17821 cccaaacggc ccgcggggtc gtacaccttt ggcctgggtt cttgcgctgc caaaggtggc
    17881 gtgtggtcgg gcgtgtcagc gcgtcgtacc gggttcgtcc aggaaagcgg cgaggtcccc
    17941 gagcaagccg gagagccgct ccagctcctc ctcgcccata cgctccgcca ggtcggcctc
    18001 tatctcggcc aggcacccgg cggcggcctc gatgccccga cggccagctt cggtcaggga
    18061 cacgatcctg gcccgcccgt ctctggggtc ggggcgcctc gtgacatacc cgcgcccctc
    18121 gaggtaggag actagctccc ccatggattg cttcgtcatc cccgccgcct cggcgagttc
    18181 ggtcacccgc gaccccccct tttccaagta acggaacacg gcgtagtggg ccggcctcac
    18241 gtcgccgtag cccgccgctc gcaacccctc gtagagtcgt acctccagcg cgcgggagcc
    18301 ctccaccagt aatctcaaga ggctgcgccg cccctggtcc gccgtcgaag caccgttctt
    18361 tccaccccgc cttgacacga aagacaggat accttaccgt atataggaag gcaacctgac
    18421 tagtaggaga ttgatgatgg aagaggcggg gtactcgcac tcgggcgcgc agtcgccggg
    18481 gatggtatcc ggcggggatg gcgagctggt gcaccgggac gggcaccggg tggtgttcct
    18541 ggaagagggc atggatggac gcggcgcgta cctgatgatc gagcacgtgt ggacgcggcc
    18601 cgggatgatg gccggtccgc actggcaccc ggtgctccag gagacgttta gcgtgcgaga
    18661 gggcaggatg cgcttccggg tggacgagag ggagttcgag ctggggccgg gggagcgcgt
    18721 caccgtgcgc cccggggagg tgcaccggtt ctggaacgag ggtgagggac gtctgacggc
    18781 catccacgag gtgcgaccac cggggcatca tcgggagatg ttcgaactgt gggacaggtt
    18841 ggatctcgcg ggcaagacca accggcaagg cgtaccgaag gaccctctgg cgctgggctt
    18901 gttgtgggag ctgcaggacg gctacctggc cggcgtaccg gcgctcctcc agaggctcgt
    18961 tttaggtagt ctcgcccgtt tggcccgggt cgtcggttac caggagcgtc tgattggtag
    19021 ttcgtcgcgc aaagacgaga gggttcgggc atgagcgccg gtcaaacgcg gacgcacgcg
    19081 aagctcgccg gtcggcgggg cccggccgct ttggtggact ggcaatcggc cctgtacgcg
    19141 ttcgtgcttc tcctgggctg cgtattcttg gttcccctga cgaagttctg gtgggtcgtg
    19201 ctcctcttgg gggtcgcggc gccgattgcg ctcgcggtgc taagcggtgg caccacgagg
    19261 gttgaaaagc caggattcgg gagagagtcg gaggaggagc tcctggcgct ggccgaacgg
    19321 ggctccctga cacccacgac ggccgccatg cgcacctccc tcacggtcga gcaggcctcg
    19381 aagatgctag ac
//
LOCUS       DATMVF010000132         9118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_294974, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000132 DATMVF010000000
VERSION     DATMVF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294974"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VN178_06860"
     CDS             <1..946
                     /locus_tag="VN178_06860"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWS80730.1"
                     /translation="FGRLASDADVVIESFRPGTLERWNLGYERLSEINPGLVLARVTG
                     FGQFGPYKDRPGFGTLAESMSGFAHVMGQPDGPPTLPPFGLADGIAALASAVAILTAL
                     RARDAGGRGQVVDLAIIEPIFTVLGPQPTVYDQLGEIQQRSGNRSVNNAPRNTYQTKD
                     GHWVAVSTSAQNIAERVMRLVGHPEYIDEPWFAKGSERARHADELDAAVGAWISDRTQ
                     EEVVAAFEEAGAAVAPIYDIEGIMQDPQYEALNSIISVDDPELGPTKMQNVLFRLSET
                     PGEVRWSGPTLGEHNEEVYGELGIGEHELDELSEKGIL"
     gene            943..1767
                     /locus_tag="VN178_06865"
     CDS             943..1767
                     /locus_tag="VN178_06865"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWS80731.1"
                     /translation="MKPPVRSYLYVPGDDGRRIEKAFASEADAVVLDLEDAVAPNRKE
                     EARETVARFLESEPEKPVFVRVNAVGTEISATDIYVVAVRNLAGLRLPKTESVESVRL
                     VAETLEELGSEARIHCLIESALGLERAFEIATSHERVAGISLGEADLSADLGVGDEAG
                     LLYARSRIVSASRAAALPHPVQSVYTLVQDLDELRRSTEAGKRLGFVGRSTIHPSQVS
                     VVNDVFTPTEVEVSEAEDLLDRLGNEGGAFVLQDGRFVDRAVVESARLTLALARGR"
     gene            1772..2584
                     /locus_tag="VN178_06870"
     CDS             1772..2584
                     /locus_tag="VN178_06870"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWS80732.1"
                     /translation="MRNGLLEAIEGGIEVYDLAQPLAAETPHSPNHPPFRMALMRRHG
                     DTVRADGSSAASELISTGGHTGTHVDALAHVSDMGKLHGGAPADEAQQGGRFSMLGVD
                     TIEPLVCRGVLLDVAGLHGVDVLPGGYGITVEDLASAEREAGVEIRAGDVALIRTGWA
                     RHFGDAGVFLGHESGVPGPTEEAARWLADRGVRATGAETIAYEQIKPEVGHALLPVHR
                     LLLAERGIHIIEVMNLSGLAAAGVHEFAFVLAPLKIVGGTGSPVRPLALVQR"
     gene            2581..4026
                     /locus_tag="VN178_06875"
     CDS             2581..4026
                     /locus_tag="VN178_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWS80733.1"
                     /translation="MTQETLLQEISSFAAGVRDGDLGPEILQDARRRTADIIGIALAA
                     SWMEPARIVGGMIEGWGGAEQAGVIGGGERYPAASAALVNGTLAHCLDYDDTHLPSVL
                     HPSAAVVPAALAAAEASGATGRDLLAAVAAGDELVVRVGMAGYDPALGNSIFFEKGLH
                     ATSIAGTLGAALAAAMVYGLEERELGHAVAISASMGAGIIEANRTGGTVKRIHCGWAA
                     HAGVTAAELAARGLTGPPTAFEGRFGFLQAYLDDKADAEALTRGLGERWEVTRIFFKP
                     YPANHFTHAGIDAAISLREQGLDIEELEEIELGVASPVLRTIARPEDEKARPKTGYAA
                     QFSGPFTVATALVGGGGLGVSLDDFTDGDVEDPIKLDLASRVRCFADEECDSIFPNQF
                     PAVLRVQLKNGEVRESRVSHNRGGPENPLSDEELEVKFRTNAGRALPARQVEELRCAL
                     EALGEADTVGDVMRLAGTRDSVPSDQGHAGG"
     gene            complement(4077..5411)
                     /locus_tag="VN178_06880"
     CDS             complement(4077..5411)
                     /locus_tag="VN178_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80734.1"
                     /translation="MERLGGEQEGQTSSIRQVALASFIGTAIEWYDFFLYGTAAALVF
                     PALFFPEFSETAGVLLAFSTYAVGFAARPLGGIVFGHYGDRIGRKAMLILSLLIMGIA
                     TFLIGLLPTYQAIGVLAPLLLVVLRFCQGIGVGGEWGGAVLMAVEHSPEGRRGFFGSW
                     PQMGVPAGLLLANLVYFGSSTLLPGAWGWRVPFLLSIVLVGVGLFIRLRIMETPAFTQ
                     VKESGTEAPMPILDVLRTHPKNVLLAMGMRVAENGTFYVLTVFVLAYIEGELGMDQST
                     GLVGVIIAAAIGLATIPLFGALSDRVGRRPVYLFGAAFSLLFAFPFFWLLNTGVAPLI
                     WIAIVLGVNLGHDSMYGPQAAYFSELFGTRVRYSGASLGYQLASVFAGGLAPLIATAL
                     LASFGWGAVAAYTAAMALITVISVILASETYQGDITETRVEEHRLIAEGQGE"
     gene            complement(5593..6198)
                     /locus_tag="VN178_06885"
     CDS             complement(5593..6198)
                     /locus_tag="VN178_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HWS80735.1"
                     /translation="MKSVEFYYDLVSPYSYLAYARVGRICEENGTELVLRPMLLGAVH
                     KAVGLQAPIETRPKAGYQAQDIRRWARYYGLPLQFPDPFPFRTLKTMRAAMFLQEREE
                     FEAFTREAFTLYWEEGGAPGGLQETDEDGPISSVARRIGVDPEEVLREAASPKAKQAL
                     KVATSEAVERGVFGAPAFFVGDEMFWGNDRLHFVEAALQQE"
     gene            complement(6568..7470)
                     /locus_tag="VN178_06890"
     CDS             complement(6568..7470)
                     /locus_tag="VN178_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWS80736.1"
                     /translation="MRSFILLALAGLGAQLVDGSLGMAYGVSSTTLLLFVGVAPALAS
                     AAVHIAEVGTTAVSGISHASFGNVDWSKIAWMAVPGGIGAFGGAYLLVYASTAEATAG
                     LVEPVVAIFLFCLGVYVLSRFAFRRRERPVDVRPISRKFLTPLGFVAGFLDAMGGGGW
                     GPISTPTLLSSGRMEPRKIIGTVDTSEFVVAFCASLGFLFSLTFSEIPWQVVGALLVG
                     GIFAAPLAAFIVRLLPARILGTAVGGVILIVNMKTFFEAIGVTGSAATAGYLLIVTVW
                     AAAIIFAITAIRRDRTVGTEGQPA"
     gene            complement(7619..8521)
                     /locus_tag="VN178_06895"
     CDS             complement(7619..8521)
                     /locus_tag="VN178_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWS80737.1"
                     /translation="MTNFLLLAIVGLIAQLVDGSLGMAYGVTSQSLLLTIGIAPALAS
                     ASVHIAEVGTTAASGVSHWRFKNVDWAKVWPLAIPGAIGAFFGAVVISSFITAEAAEP
                     IVAIFLFGLGVFVLARFSFRRHERPVRVRPVSRGFLAPLGLFAGFLDAAGGGGWGPIS
                     TPTLLSSGRMEPRKIIGTVDTSEFLVALSASIGFLFALTLAAIPWTIVGALLLGGVIA
                     APIAAYIVRIMPARILGTAVGGIILITNMKTFLEAVGVGGPLAVLIYIAIVAVWIAAL
                     VFSIQVNRREKSGAAGDPSTSRVV"
     gene            8784..>9118
                     /locus_tag="VN178_06900"
     CDS             8784..>9118
                     /locus_tag="VN178_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWS80738.1"
                     /translation="MDRPNVVMIVADTFRRDHLGAYGNPFISTPHIDEFARSSVVFDQ
                     HVVSSFPTMPARADMLTGTFSYTHMGWEPLPRHLTTLPDVLSKAGYHTMGVVDTPFFI
                     RNGFGYDRG"
BASE COUNT         1763 a         2946 c         2946 g         1463 t
ORIGIN      
        1 cttcgggcgt ctggctagcg acgcggacgt cgtgatcgag agtttcaggc ccggcaccct
       61 ggagcggtgg aacctgggct acgagcgcct cagcgagata aaccccggcc tcgtcctcgc
      121 ccgcgtcacc ggcttcggcc agttcggccc gtacaaggac agacccggct tcggcaccct
      181 ggccgagtcc atgagcggct tcgcccacgt gatgggccag cccgacgggc cgccgacgct
      241 tccgcctttc gggctggcgg acggcatcgc agcgctggcg agtgcggtcg ccatcctgac
      301 cgccctcagg gcccgcgacg ccggcgggcg cggtcaggtc gtcgaccttg cgataatcga
      361 gccgatcttc accgtcctcg gcccgcagcc gacggtctac gaccagctcg gtgagattca
      421 acagaggagc ggcaaccgct ccgtaaacaa tgcaccgcga aacacctacc agacgaagga
      481 cggccactgg gtcgccgtct ccaccagtgc ccagaacatc gccgagcgcg tgatgcgcct
      541 ggtcgggcac cccgagtaca tcgatgagcc gtggttcgcg aagggctcag agcgggccag
      601 gcacgcagac gagctcgatg cggcggtggg agcctggatc tccgatcgca cccaggagga
      661 ggtcgtggcg gccttcgagg aggcgggggc ggccgtcgcc cccatctacg acatagaggg
      721 catcatgcaa gacccccagt acgaggcgct gaactccatc atcagcgtgg acgaccccga
      781 gctcggcccc acaaagatgc aaaacgtcct cttccgcctc tccgagactc ccggcgaggt
      841 cagatggtca ggacccaccc tgggagagca caacgaagag gtctacggag aactaggcat
      901 cggcgagcac gaactggatg aactctccga gaagggcatc ctgtgaaacc gccggttcgg
      961 agctatctct acgtgcccgg tgacgacggg cgaaggatcg agaaggcctt cgcgagcgag
     1021 gccgacgccg tcgtcctcga cctggaagat gccgtcgccc cgaaccgcaa ggaggaggcc
     1081 cgcgagacgg tggcacgctt tctcgagtcg gagccagaga agccggtctt cgtgagagtg
     1141 aacgcggtgg gaacggagat ctcggcgacg gacatctacg tggtggcggt acgcaacctc
     1201 gccgggttgc ggctgccgaa gacagaatcg gtcgaaagcg tcaggctggt ggcagagacg
     1261 ctcgaagagc tgggcagcga ggcccggata cactgcctga tcgaatccgc ccttggcctc
     1321 gaacgcgcgt tcgagatcgc cacctcccac gagagggtcg caggcataag cctcggcgag
     1381 gccgacctct cggcggacct tggcgtgggc gacgaggccg gacttctcta cgcacgctcg
     1441 cgcatcgtaa gcgcctcacg tgcggccgcg ctgccgcatc cggtccagag cgtgtatacg
     1501 ctggtccagg atctcgacga attgcgccgt tctactgaag caggaaagag actcggcttc
     1561 gtgggacgct cgacgataca cccttcccag gtctccgtcg tcaacgacgt atttacgccg
     1621 acggaagtgg aggtatccga agccgaggat ctgctcgacc ggctcggcaa cgaggggggc
     1681 gcgttcgtgc ttcaggacgg ccggttcgtg gacagggcgg tagtcgagtc cgcgcgcctc
     1741 acgctcgccc tggcgagggg gaggtaacgg cttgaggaat ggcttgctcg aagcgatcga
     1801 gggcggcatc gaagtctatg acctggctca gccgctcgcc gccgagacac cacactcgcc
     1861 caaccatccg ccgttcagga tggcactcat gcgccgccac ggcgacacgg tccgcgccga
     1921 cggcagctcc gccgcgagcg agttgatctc gaccggcggc cacacgggga cccacgtcga
     1981 cgccctggcc cacgtctccg acatgggtaa gctgcacggc ggcgccccgg cggacgaagc
     2041 ccagcagggt ggacgcttct cgatgcttgg cgtggacacc atcgagccgc tggtctgccg
     2101 cggcgtgctg ctcgacgtgg ccgggctgca cggcgtggac gtgctgcccg gtgggtacgg
     2161 cattacggtg gaggatctcg catcggccga acgagaggcc ggcgtggaga tcagggccgg
     2221 tgacgtcgcc ctcatcagaa cgggctgggc gcggcacttc ggcgacgccg gggtctttct
     2281 gggacacgag agtggggtgc ctggcccaac cgaggaggcc gcccgctggc tcgccgatcg
     2341 gggcgtgcgg gcgacgggcg ccgagaccat agcctacgag cagataaagc ccgaggtggg
     2401 gcacgctcta ctgcccgttc accgcctgct ccttgcagag cgcggtatcc acattatcga
     2461 ggtcatgaac ctctcgggac tcgcggcggc cggtgtgcac gagttcgcat tcgtcctcgc
     2521 gccgctcaag atagtgggtg ggacaggctc cccggtacgc ccactggcgc tggtgcagcg
     2581 gtgacacaag aaaccctgtt gcaggagata tccagtttcg ccgctggcgt acgggacggc
     2641 gacttgggac ctgagatcct ccaggatgcc aggcggcgga cggccgacat catcgggata
     2701 gccctcgcgg cgagctggat ggagccggcc aggatagtgg gagggatgat cgaaggttgg
     2761 ggcggtgcgg agcaggcggg cgtcatagga gggggcgaga ggtatccggc cgcgagcgcc
     2821 gccctcgtga acggtaccct ggcacactgc ctcgactacg acgacacgca cctcccttcc
     2881 gtgttgcacc cgagcgctgc ggtcgtgccc gcggcactcg cggcggccga ggcctcaggg
     2941 gctacgggcc gggatctcct ggctgccgtc gccgctggag atgagttggt cgtgcgcgtc
     3001 ggcatggccg gctacgaccc tgctcttggc aactcgatct tcttcgagaa gggtttgcac
     3061 gccacctcca tcgccgggac tctcggcgct gcgctcgctg cggcgatggt ctacgggctc
     3121 gaggaaaggg agctcggtca cgccgtagcg atctcggcga gcatgggcgc gggtatcatc
     3181 gaggcgaacc gtaccggagg gacggtcaag cgcatacact gcggctgggc cgcacacgcc
     3241 ggcgtcacgg ccgccgagct cgccgcccgc ggcctgaccg ggcctcctac ggccttcgaa
     3301 ggccggttcg gctttctgca agcatatctc gacgataagg ccgacgccga agcgttgacg
     3361 cgaggactcg gagaaaggtg ggaggtgaca aggatcttct tcaagcctta ccccgcgaac
     3421 cacttcaccc acgcagggat agacgcggcg atatcgttgc gcgagcaggg tctggatatc
     3481 gaggaactcg aagagatcga actgggcgtc gccagccctg tcttgcgcac catcgcccgg
     3541 cccgaagacg agaaggcacg tccgaagacg ggttacgccg cccagttcag cggccctttc
     3601 accgtggcga ccgcgctcgt cggaggcggt ggcctcggtg tctccctgga cgacttcacg
     3661 gacggtgacg tcgaggaccc cataaagctc gacctcgcct cgcgtgtgcg ctgcttcgcg
     3721 gacgaggagt gcgacagcat cttccccaac cagtttccgg ccgtgctgcg tgtccagctg
     3781 aagaacggcg aagtgcggga gtcgcgcgta tcgcacaacc gcggagggcc ggagaatccc
     3841 ttgagcgacg aggagctcga ggtcaagttc cgaacgaatg ccgggcgcgc cttgccggcc
     3901 aggcaagtcg aggagctgcg ttgcgcgctg gaggcgctcg gcgaggcgga caccgtggga
     3961 gacgtgatgc ggctagcagg aacgagagac tcggtcccat ccgaccaggg acacgccgga
     4021 ggctaatcta cgcgacacgg agaatcgagg cgaagcgcga cctcttacta tgtcccttat
     4081 tctccctggc cctccgcgat gagccggtgc tcttccaccc gtgtctcggt tatgtccccc
     4141 tggtatgtct ccgaggcgag gatgaccgag atcacggtga tgagcgccat cgccgccgtg
     4201 taagccgcga ccgcccccca gccgaagctg gcgagcaggg ctgtagcgat gagcggcgcc
     4261 aggccgccgg cgaagaccga ggccagctgg tagcccagag aagctccgga gtagcgcacc
     4321 cgggtgccga agagctcgga gaagtatgcc gcctgaggcc cgtacataga gtcgtgaccg
     4381 aggttcacgc cgaggacgat ggcgatccag atcaggggcg caacgcccgt gttcaagagc
     4441 cagaagaatg ggaaagcgaa gagcagcgag aaggcggccc cgaacaggta cacaggacga
     4501 cgtcctacgc ggtcggagag cgccccgaaa agggggatgg tagcgagccc gatggcggcg
     4561 gcgattatga cgccgaccag gcccgtactc tgatccattc ccagctcgcc ttctatgtag
     4621 gcgagcacga agacggtcag gacgtagaaa gtcccgttct cggcgacgcg catccccatc
     4681 gcgagcaaga cgttcttcgg gtgggtgcgc aacacgtcca gtatcggcat gggagcctct
     4741 gttccggact ctttgacctg cgtgaaagcc ggggtctcca tgatcctcag ccggataaag
     4801 aggccgacac cgacgagcac gatgctgagc aggaacggca cgcgccagcc ccacgccccc
     4861 ggcagcaggg tggaggagcc gaaatagacc aggttggcga gcagcagacc cgccgggacc
     4921 cccatctgcg gccagcttcc gaagaagcct cgccggccct ccggggagtg ctcgaccgcc
     4981 atcaggacgg ccccgcccca ctccccgccg accccgatgc cctgacagaa gcgcaacacg
     5041 acgagcaaga gcggagccag gacgccgatg gcctggtagg tcggcaagag ccctattagg
     5101 aacgtcgcga tacccatgat gaggagcgac aggatcagca tcgccttgcg tcctatcctg
     5161 tccccgtagt gcccgaagac tatgccgccc agaggcctcg ccgcgaagcc aaccgcgtag
     5221 gtcgagaacg ccaggagcac gcccgcggtc tctgagaact cggggaagaa cagcgcggga
     5281 aagactaacg ccgccgctgt cccgtatagg aagaagtcgt accactcgat ggcggtgcca
     5341 ataaagctcg ccagcgccac ctgacggatc gagctggtct gcccctcttg ttctccgcct
     5401 aatctctcca cacgagcctc ctttgcatgc ctgagcccga tgcggactcc ggcagtcccc
     5461 actacctacc aaatactact cacgccctcc cacacgatgc aacccgctga cgtgttcctg
     5521 tcaaaatgga cctcttcgcc agtccgacgc gggggtcctg gctacccaaa gtagggtgac
     5581 ccgggacggc tcttactcct gctgcagggc agcttctacg aagtggagcc ggtcgttgcc
     5641 ccagaacatc tcgtcgccga cgaagaaggc cggggccccg aagacgcccc gctccaccgc
     5701 ttctgaggtc gcgaccttga gcgcctgttt cgcctttggg ctcgcggcct ccctcagcac
     5761 ctcctccggg tcgaccccta tccgcctggc gacggatgag atcggtccgt cctcgtcggt
     5821 ttcctggagt cctcccgggg cgcctccctc ctcccagtag agcgtgaacg cctctctggt
     5881 gaaagcttcg aactcttccc tctcttgcag gaacatcgct gcgcgcatcg tcttcagggt
     5941 gcggaagggg aaggggtcag ggaactgcaa cggaaggccg tagtatcgtg cccagcgacg
     6001 gatatcctgc gcctgatagc cggctttggg ccttgtctcg atcggggcct gcagccccac
     6061 ggctttgtgg accgcgccca gcaacatcgg ccgcagtacc agttccgtgc cgttctcctc
     6121 acagatacgc cccacccgtg cgtacgccag gtaggagtag gggcttacga ggtcgtagta
     6181 aaactctacg ctcttcatgc cggctcctct cgctcaagcc ataaggtaca acacggccgg
     6241 taccagacgc gaggtcagcc cagattcttt tttgccgatg ggcaacacgg agatcacgac
     6301 gcccgacgct acgatcccgg cggacgacgg ttgccgctgg ctctccctag gcaccagcag
     6361 gaaccacggc cggcgttggt gcgccataaa ggtctgcggt acgcatgaga aggcgcgccg
     6421 gtacgtcgag aaacgcgcgg ccaggcgtga ctcccaaccg cgcggcgacg ctcgggtacc
     6481 tcgccgtgct tgcgcaacgg aaagcggcgg ggtccggatc gtggtccgga ccccgccgct
     6541 cgggtcgagg gcgttgtgtc agagggatta ggccggctgg ccctccgtcc caacggtgcg
     6601 gtccctgcgg atcgccgtga tcgcgaagat gattgccgcc gcccacaccg tgacgatcag
     6661 gaggtagcct gctgtggccg ccgatcccgt caccccgatg gcctcaaaga acgtcttcat
     6721 gttgacaatg aggatgacgc cgccgaccgc cgttccaagg atgcgagccg ggagcagcct
     6781 gacgataaag gccgcgagcg gggccgcgaa gataccgccc acgagcagag cgccgaccac
     6841 ttgccatggg atctcggaga aggtcaggct gaagaggaag ccgaggctcg cgcaaaacgc
     6901 caccacgaac tcgctggtgt cgacggtgcc gatgatcttg cgcggctcca tccgtcccga
     6961 agagagcaac gtcggcgtgg agatggggcc ccatccccca ccgcccatcg cgtcgaggaa
     7021 cccggcaacg aagccgaggg gagtgaggaa cttgcggctg atcggtcgga catcaacggg
     7081 acgctccctc ctcctgaagg cgaagcggct gaggacgtaa acgccaaggc agaacaggaa
     7141 gatggccacg accggctcga ccaggcctgc cgtagcctcg gcggtggaag cgtacaccag
     7201 cagatacgcc ccaccgaaag cacctatccc accggggacc gccatccagg cgatcttgga
     7261 ccagtccacg ttgccgaagc tcgcgtgcga gatgccagat accgccgtcg tcccgacctc
     7321 cgctatgtgg accgccgcgg acgccagcgc gggcgccacc ccgacgaaca ggagcaacgt
     7381 ggtcgacgag accccgtaag ccatcccaag agatccatcc accaactgcg cccctagacc
     7441 cgccagcgca agaagtatga aacttctcat ctaccacaac ctccttaaca tcattttgtc
     7501 tatagtcttt gtttataatg tcgcgctgga ctttatatcg ctatgtgtct ggcgtcaagt
     7561 gcgcggcgga agtttttatt ggctcccgcc gcgcgttgcc gaagggcctg tgaaagatct
     7621 agacgacccg gcttgtggag gggtctccgg cagcgccgct cttctccctg cgattgacct
     7681 ggatcgagaa cacgagggcg gcgatccaga cggccacgat cgcgatgtag atcagtacgg
     7741 cgagcgggcc gccgacgcct acggcctcca agaacgtctt catgttcgtg atgaggatga
     7801 tgccgccgac ggcggtgccg aggatgcggg ctggcatgat gcgcacgatg tatgcggcga
     7861 tcggggcggc tatgacgccg ccgaggagga gcgccccgac gatcgtccaa gggatcgcgg
     7921 cgagcgtgag ggcgaacagg aagccgatgc tcgcgctgag ggcgacgagg aactcgctgg
     7981 tgtcgacggt gccgatgatc ttgcgcggct ccatccgtcc ggaagagagc aacgtcggcg
     8041 tggagatggg gccccatccc ccaccgccag cggcatcgag gaatccggcg aacaggccga
     8101 gaggagccaa aaaaccccgg gagaccggcc tgaccctgac gggacgctcg tgccgcctga
     8161 aggagaagcg ggcgagcacg aagacgccaa gcccgaacag gaagatggcc acgatcggct
     8221 ccgcagcctc tgcagtgatg aacgaactta tgaccacggc gccaaagaaa gctcctatcg
     8281 caccgggtat cgcgagcggc cagactttcg cccaatccac gttcttgaac cgccagtggg
     8341 agacaccgga tgccgccgtc gtgcctacct cggcgatgtg tacgctcgcc gacgcaagtg
     8401 ccggcgcaat gccgatcgtc aatagcaggc tctgcgaagt caccccgtaa gccatgccaa
     8461 gcgacccgtc gaccaactgc gcaatgagcc ccacaatcgc caacaacagg aaattagtca
     8521 tctatcccat ccctccactc ttcactttgt ctactatttc taccttgtct accatttcga
     8581 tgtaaaatac gctgcgcacg atatagcccc aaaggggcta tgtcaaggct ctggatggct
     8641 cagaggccca aacttcgtaa aaaaacttcg gatctcttaa aatttctacg taaatgctct
     8701 tgactcaccg cccgacctgt tctagagtcc cctcgctatt gttaataaac tttgtagaca
     8761 aagtaagcaa gggaggagaa gagatggatc gtcccaacgt ggtgatgatc gtggcggaca
     8821 cgtttcgcag ggatcatctg ggggcatacg gcaacccgtt catcagcacg ccgcacatag
     8881 acgagttcgc gcgttcttct gtggttttcg accagcacgt ggtctcttcc tttcccacga
     8941 tgccggcgcg ggcggacatg ttgacgggga cgttttcgta cacgcacatg ggttgggagc
     9001 ccttgccgcg gcatctgacg acgttgcccg acgtactctc caaagccggt taccacacga
     9061 tgggtgttgt ggacacgccg ttcttcattc gcaacggttt cgggtacgac cgcggctt
//
LOCUS       DATMVF010000133         5162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_13030, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000133 DATMVF010000000
VERSION     DATMVF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13030"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VN178_06905"
     CDS             <1..1203
                     /locus_tag="VN178_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HWS80739.1"
                     /translation="AIEERAEELVRAESENTGKPFGLTMEEEIPAGVDQIRFFAGAAR
                     VLEGKSAGEYLEGYTSFIRREPVGVIGQVTPWNYPMMMAIWKFAPAIAAGNTIVIKPS
                     DTTPVSTVMMAEIAAEFLPDGVFNVVCGDRDTGRMLVQHPTPQMVAITGSVRAGMQVA
                     EAAASDLKRVHLELGGKAPVIIFDDADIEAAAEEIAGAGYFNAGQDCTAATRVLAHPG
                     IHDDFVAAITDQARNTTTGHPDDEVDYGPLNNANQLEHVSGMVDRLPDHAEVTVGGER
                     FGEQGFFYSPTVVAGLRQDDEASQNEIFGPVVTVQSFSDEDEAVRWANGVKYGLASSV
                     FTTDHGRAMRVSRRLDFGCVWINTHIPLVAEMPHGGFKHSGYGKDLSMYGLEDYTRIK
                     HVMTNLEA"
     gene            1381..2847
                     /locus_tag="VN178_06910"
     CDS             1381..2847
                     /locus_tag="VN178_06910"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWS80740.1"
                     /translation="MSMATSEQSVVDSVNKQLYIGGEWRDASGGATLTVEDPSTAEPL
                     CEIADGSSEDALDALASADQMQAEWGKTAPRERGEILRRAFEKLVERTDELALLMTLE
                     MGKALAESKAEITYAAEFLRWFSEEAVRIEGRYAIPPAGAGRMLTMKQPVGPCLFITP
                     WNFPMAMGTRKIGPAIAAGCTMVMKPAQQTPLSMLALAGILEESGLPGGVLNVVTSSS
                     SSGTMGPLIADPRARKLSFTGSTEVGRKLVAQSADQLLRVSMELGGNAPFLVFDDADV
                     DAAVEGAMVAKMRNIGEACTAANRFHVADGVAGEFAEKLAKRMEAMKVGRGTEEGVEV
                     GPLIDGDQREKVADLVDDALGKGARVLVGAEKMEGSGYFYRPTVLDDIPEDATLLKEE
                     IFGPVAPVASFDSEEAAVAAANDTEYGLVAYVYTSDLKRALRVCEGIETGMVGLNQGM
                     VSNASAPFGGIKHSGFGREGGIEGIEEYLETKYVAINL"
     gene            2858..3295
                     /locus_tag="VN178_06915"
     CDS             2858..3295
                     /locus_tag="VN178_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80741.1"
                     /translation="MTTVRPIRGEDRDAWLHLWQGYNDFYRNSPTEEVTRETFRRVRD
                     GSDGLFGLVAEDDEGRLVGLAHLVFHPSTWTTATYCYMEDLFVSREGRGSGAGRALIE
                     GVYAEADRRGADRVYWHTQQYNAPARSLYDVVGTATSFVVYER"
     gene            3307..4749
                     /locus_tag="VN178_06920"
     CDS             3307..4749
                     /locus_tag="VN178_06920"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWS80742.1"
                     /translation="MTENGRLSVVNPATGENIEEIPISSREEVDAAVERAREGFREWQ
                     GFAGLDRAEVLHEISARMREHADQLAEVMTQEGGKPFIENRDEVTWTAACFDYYAEIA
                     RDSVGYLPAPIEPQQLALVVKEPVGTVACIVPWNYPLLLAAWKVAPALAAGNAVLLKP
                     SEETPLATRRLAELMHELPDGLLQVVYGAGEVGSMLVRHPGVDMVAFTGSQETGRRVG
                     LAAAERLIPANLELGGKDPFIICDDVDIEIAAQGAVWAACLNAGQVCTSSERFYVMSE
                     VADDFVEASKEFVESLNIGDPMDRSTDIGPMINAKSREKVERHVGEAVDDGATLVAGG
                     GRYGERGYFYKPTVLTGVTPSMTVMRDETFGPVVPVVEVGSLDEAIEQANSVPYGLGA
                     NVYTQDFEKMLKCMRELKAGTVWINDPLTDNDAAPFGGQKGSGIGRELGREGLEAFQE
                     SKHVHIDPKVEKKEWWYPYGKDDDPGQRTL"
     gene            4751..>5162
                     /locus_tag="VN178_06925"
     CDS             4751..>5162
                     /locus_tag="VN178_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80743.1"
                     /translation="MTAPQELASGVYRVDAFRFSNFINVLLLENDDGWTLVDTGVAGS
                     VGRIGEALASLGSGPSDLKRIFITHQHDDHTGGLKGLLEWAPHAEVGAPEHEAKVISG
                     ELPYDPQNNRFLRYMTRNAEPPGVPVARTLGEGDL"
BASE COUNT          979 a         1625 c         1727 g          831 t
ORIGIN      
        1 gcgatagagg agagggccga ggaattggtc cgggccgaat cagagaacac cggaaaaccg
       61 ttcggactca ctatggagga ggagatcccg gccggggtgg accagatccg ctttttcgcc
      121 ggcgccgccc gagttctgga aggcaagtcc gccggagagt atctcgaagg gtacacgtcg
      181 ttcatccgcc gcgagccagt aggcgttatc ggccaggtaa cgccgtggaa ctacccgatg
      241 atgatggcga tctggaagtt cgctccggcc atagccgctg gcaacaccat cgtcataaag
      301 ccctccgaca ccaccccggt ctccacggtc atgatggccg agatcgcggc tgagttcctg
      361 cccgatggtg tgttcaacgt ggtctgcggc gatcgggaca caggaaggat gctggtccag
      421 caccccacgc cgcagatggt cgcgatcacc ggttcggtca gggccggcat gcaggtcgcg
      481 gaggcggctg cctccgacct caaacgggtc cacctcgagc tcggcgggaa ggcgccggtg
      541 atcattttcg acgatgccga catagaagcg gcggcagagg agatcgctgg tgccggctac
      601 ttcaatgccg gtcaggactg caccgcggcc acccgcgtgc tcgcccatcc tggcatccac
      661 gacgacttcg tggcggccat caccgaccag gccaggaaca cgacgacggg gcaccccgac
      721 gacgaggtgg attatggccc gctaaacaac gcaaaccagc tcgagcacgt ctccggcatg
      781 gtcgacaggc tccccgacca cgccgaggta accgtcggcg gcgagcgctt cggcgagcag
      841 ggcttcttct actcccccac cgtggtcgcg ggactcaggc aggacgacga agcttctcag
      901 aacgagatct tcggtcccgt cgtcaccgtc caatcgttct cggatgagga cgaggcggtc
      961 agatgggcca acggcgtcaa gtacggcctc gcctccagcg tcttcaccac ggaccacggg
     1021 cgggccatgc gggtaagccg caggctggac ttcgggtgcg tgtggatcaa cactcacatc
     1081 cccctcgtgg cagagatgcc ccacggcggc ttcaagcact ccggctacgg caaggacctc
     1141 tccatgtacg gcctcgaaga ctacacgcgc atcaagcacg ttatgacgaa cctcgaagcg
     1201 tagaggaggc tacagccatc agcggtcagc tttcagcctt cagccagaaa ccaatggctg
     1261 acagctgagg gctgatcgct gacagcgcgg ttcgcaaagg cttggacttt gcgaaccgct
     1321 ttagcggagg accgaacagg gtaacctgaa gcctgaagcc tttgacgaaa ggagatcccg
     1381 atgagcatgg caacgtccga gcaaagcgta gtagatagcg taaacaagca gctctatatc
     1441 gggggcgagt ggcgcgacgc ctcaggtggc gccacgctga ccgtcgagga cccttccacc
     1501 gccgagccgc tctgcgagat cgcggacggg tcgtccgagg acgcgttgga cgcgctcgcc
     1561 tcagccgacc agatgcaggc cgagtggggc aagacggcgc cgcgcgagag gggtgagatc
     1621 ctccgccgcg ccttcgagaa actggtggag cgcacggatg agctggccct cctgatgacg
     1681 ctggagatgg gcaaggcgct cgccgaatcg aaggcggaga ttacctacgc cgccgagttc
     1741 ctccggtggt tctcggagga ggccgttcgc atagagggcc gctacgccat cccccccgct
     1801 ggcgccgggc gcatgctgac gatgaagcag cccgtcggcc cgtgcctgtt cataacgccg
     1861 tggaacttcc ccatggcgat ggggacgcgt aagatcgggc cggccatcgc cgcaggctgc
     1921 acgatggtga tgaagcctgc ccagcagacc cccctctcca tgctcgccct cgccggcatc
     1981 ctggaggagt caggccttcc cggcggcgtc ctcaacgtgg tgacgagctc ctcctccagc
     2041 ggtacgatgg gaccgctcat cgccgacccg cgcgcccgca agctctcctt cacgggctcg
     2101 acagaggtag ggcgcaagct cgtcgcccag tcggccgacc agctcctgcg cgtctccatg
     2161 gagctcggcg gtaacgcccc gttcctggtc ttcgacgacg ccgacgtcga cgccgcggtc
     2221 gagggcgcca tggtcgccaa gatgcgcaac atcggcgagg cgtgcaccgc ggccaaccgc
     2281 ttccacgtcg cagacggcgt ggctggtgag ttcgccgaga agctcgcgaa gcgtatggag
     2341 gccatgaagg tcgggcgcgg caccgaggag ggtgtggagg tgggccccct catcgacggc
     2401 gaccagcgcg agaaggtcgc cgacctcgtc gatgacgcgc tcggcaaggg agccagggtc
     2461 ctggtcggcg ccgagaagat ggaggggtca ggatacttct atcgccccac cgtactcgat
     2521 gacatccccg aggacgcgac gctgttgaag gaggagatct tcggccccgt agcccctgtg
     2581 gcctccttcg actccgaaga agctgccgtg gccgccgcta acgacaccga gtacggcctc
     2641 gttgcctacg tctacaccag cgacctgaag cgcgccctgc gcgtctgcga gggcatcgag
     2701 accggcatgg tcggcctcaa ccagggcatg gtctccaacg cctcggcccc cttcggcggc
     2761 atcaaacact caggcttcgg ccgcgaagga gggatagagg gtatcgagga gtatctggag
     2821 acgaagtacg tggccatcaa cctgtagagg agggcatttg acgaccgtcc gtcccatccg
     2881 gggagaggat cgcgacgcct ggctacacct ctggcagggc tacaacgact tctaccgtaa
     2941 cagccctacg gaggaggtca cacgtgagac tttccggcgc gtgcgagatg ggtcggacgg
     3001 gctcttcggc ctggtcgcgg aggacgacga aggcaggctc gtcggcctgg cgcacctcgt
     3061 tttccatccc tccacgtgga cgacggcgac ctattgctac atggaggatc tgttcgtgtc
     3121 gcgagaaggg cgcggctccg gggccgggcg cgccctgatc gagggtgtct acgccgaggc
     3181 cgaccggcgc ggcgccgatc gcgtctactg gcacacccag cagtacaacg ccccggcccg
     3241 gtcgctatac gatgtggttg gcacggccac gtcgtttgtg gtctacgaga ggtgaacgga
     3301 ggagagatga ccgaaaacgg acgcctgagc gtcgtcaacc cggctacggg agagaacatc
     3361 gaggagatac cgatctcttc ccgtgaggag gtcgacgctg ccgtcgagcg ggcgcgggaa
     3421 ggttttcgcg agtggcaggg gttcgccggg cttgaccggg ccgaggtgtt gcatgagatc
     3481 tctgcgcgca tgcgggagca tgcggaccag ctcgctgagg ttatgacgca ggagggcgga
     3541 aagcccttta tcgagaaccg cgatgaggtt acgtggacgg ccgcctgctt tgactactac
     3601 gctgagatcg cccgcgactc cgtcgggtac ctgccggcgc ccatcgagcc gcagcagctc
     3661 gccctcgtcg tgaaggagcc cgtcgggacg gtggcctgca tcgtgccctg gaactatccg
     3721 ctgctgctcg cggcgtggaa ggtcgcaccg gcgctcgccg cggggaacgc ggtgctcctg
     3781 aagccgtcgg aggagacacc gctcgctacg cggcgcctcg ccgagctcat gcatgaactg
     3841 cccgatgggc tgctgcaggt cgtgtacggg gccggcgagg tcggttccat gctcgttcgg
     3901 caccccgggg ttgatatggt cgcgtttacc ggaagccagg agacagggcg cagggtcggc
     3961 cttgcagccg ccgaacgcct catccccgcg aatctggaac tcggcgggaa agaccccttc
     4021 atcatctgcg acgacgtgga catcgagatc gccgcacagg gcgccgtgtg ggccgcctgc
     4081 cttaacgccg gtcaggtctg cacctcctcc gagcgtttct acgtgatgag cgaggtggcc
     4141 gacgattttg tcgaggcgtc aaaggaattc gtcgagtcgt tgaatatcgg ggacccgatg
     4201 gaccgctcga cggacatagg gcccatgatc aacgcgaaga gccgcgagaa ggtcgagcgc
     4261 cacgtcgggg aggctgtaga cgatggtgct accctcgtcg ccggtggcgg gcgctacggt
     4321 gagcggggct acttctacaa gcccacggtt ctcaccggcg taacgccgtc catgacggtg
     4381 atgcgggatg agaccttcgg gcctgtggtt ccggtcgtgg aggtcgggag cctggacgaa
     4441 gcgatagagc aggccaactc cgtgccttac ggactcgggg cgaacgtgta cacgcaagac
     4501 ttcgagaaga tgctcaagtg catgcgggag ctcaaggccg ggacagtctg gatcaacgac
     4561 cccctcaccg acaacgacgc agcgccattc ggcggccaga aaggcagcgg catcggccgc
     4621 gagctcggcc gcgagggtct cgaagccttc caggagtcca aacacgtcca tatcgacccg
     4681 aaggtggaga agaaggagtg gtggtacccc tacggcaagg acgacgatcc tggccagagg
     4741 acactgtagc gtgaccgctc cccaagagct ggcgtccggt gtctacaggg ttgacgcctt
     4801 tcgcttctcg aacttcataa acgtgctcct cctggagaac gacgacggct ggacgctcgt
     4861 agacaccggt gtggccggca gcgtggggcg tatcggggag gctctagcca gcctcggttc
     4921 tggcccaagc gatctcaagc gcatcttcat cacccaccag cacgacgacc acacaggcgg
     4981 tctgaaaggt ctcctggagt gggcgccgca tgctgaggta ggcgccccgg agcacgaggc
     5041 aaaggtgata tccggcgagc ttccctacga tcctcagaac aacagattcc tcaggtatat
     5101 gacccgcaac gcagagccgc ctggcgtccc tgtcgcacgg acgttgggtg agggcgacct
     5161 gg
//
LOCUS       DATMVF010000134         6921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_160120, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000134 DATMVF010000000
VERSION     DATMVF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160120"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VN178_06930"
     CDS             <1..754
                     /locus_tag="VN178_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWS80744.1"
                     /translation="VDPDEVGATVPAEVGLISDAGTALAAVAEQAPASPPARDWTEAH
                     RAYLDSSTIPPDRAREGIDPARVVGAMREALPEDALLTNDAGNFSVFIHRYWRYNHAG
                     TQLASTNGAMGYGVPAAVAAKLAAPDRTVVACCGDGGFLMTGQEIETAVRYGAAITVV
                     VFRNRLHGTIAMHQKQALGRTAGVEIGEVDLAGYARSFGAEGHTVREPEGLAPAMEAA
                     LASDTVTLLDVVTDPDIISPSTTLSEIAGGRT"
     gene            1020..1226
                     /locus_tag="VN178_06935"
     CDS             1020..1226
                     /locus_tag="VN178_06935"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HWS80745.1"
                     /translation="MDFLRKRLPAQVGRYLEGVKFPARKEELLGRLERNGVPGPVIGQ
                     LRKRLPEGEYRGPQDVLSALRQRR"
     gene            1365..1649
                     /locus_tag="VN178_06940"
     CDS             1365..1649
                     /locus_tag="VN178_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80746.1"
                     /translation="MASESPERLEELRERFLRTLSDLGGGAEEGRPAMISMVAEGAGL
                     DPEGNPEERALSERLAAELVDVGYASAEAGSSGFLAITPEGEQALRGGTS"
     gene            complement(1801..2265)
                     /locus_tag="VN178_06945"
     CDS             complement(1801..2265)
                     /locus_tag="VN178_06945"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWS80747.1"
                     /translation="MYVKKVLLAAAVVLVVLVGAGVAYAANNNSSEEAADESYKGSVS
                     VAGKNESSAQELAKIDEAAAERAALDAAPGTVHETELETSDNGYLVYDIEIAGKDGKE
                     HEVTVDAGDGKVLHQDLEDEADEPDGRDEAADDGQDAKEDADDGPGDKEDTD"
     gene            complement(2469..2948)
                     /locus_tag="VN178_06950"
     CDS             complement(2469..2948)
                     /locus_tag="VN178_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWS80748.1"
                     /translation="MKRIMLLIASMMLALLLAAGVALAQNGVSKTCNTNCSGTDRDDQ
                     LTGTSNRNAIEGRDGADQIEGNGAKDTLNGNRGEDAIYGGNGEDKLYGGGNDDYMQGG
                     IKTDYIGTGSGDDVVAAKDGFKDQIFCGSDFDRVYVDRIDVLHRCERKLSEMPQPQF"
     gene            3295..4158
                     /locus_tag="VN178_06955"
     CDS             3295..4158
                     /locus_tag="VN178_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWS80749.1"
                     /translation="MATVAAFLIAGGCTTEPVPSAQEVVAAGDIASCRTQADEATAEL
                     VEGIDGTVLALGDVAYPRGSAANFEECYGPSWGRFKERTRPVPGNHEYFTEGATGYFE
                     YFGEAAGDPEEGYYSYELGSWHVVALNSNCEEVRCGPGSPQTRWLEEDLAANEEARCT
                     LAYMHHPRFSSGEKHGSSDGGVENLWEALYEADADVVFSGHEHNYERFSPQDPQGRVD
                     PERGIRQFVVGTGGGGGEGPISEPIVNSEVRIDGIDGVLKLTLRPKSYEWEFVPVEGE
                     SFTDSGSAQCH"
     gene            complement(4248..5711)
                     /locus_tag="VN178_06960"
     CDS             complement(4248..5711)
                     /locus_tag="VN178_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80750.1"
                     /translation="MRRIVLLLTSTAVALLLASGGILALPSERPDKTPMVDGRVRAIE
                     QVGNNTWLGGTFTRIKKHKGTVVANVSNLAVFDSKTQQYKNIAPKLGGTDSEVWDMAL
                     YGEDVLIAGSFSGPSGKGNNLVRVDGKTGRVIKWFEAPPLKSVLGAPDLGRVYGGGDS
                     LSAFEFDGSKKRPLWSRAKITIDESLYDRVHEAAYRDLERDGKTIWAACLCDAVNGKL
                     AKALVKLSTEGVHDASWVTKAARDSYGHEVVNHDGKLYLAAGGSDFLAELDKGAGGNR
                     SWVRDTSGSVQAVTVMGGQLVVGGHFQEVADHPNDDCGRRLEVQDPNDECQTRKGIAA
                     YSFNGRLENNWSPTYSGSYSLVWALYVEEARLHTGGEFKTVSGVTQSYYARFSRASIK
                     GDERPNTLAGTSNGDAIYGYGGDDRIDARGGYDTLRLGGGKDRGRGGRGNDQIFAVDG
                     SKDEIYCGPGSDRVKANPGDNVAGGCEKVGRAGTKVG"
BASE COUNT         1254 a         2194 c         2130 g         1343 t
ORIGIN      
        1 cgtagaccct gacgaagtcg gcgcgacggt ccccgccgaa gtcggcctga tctcggacgc
       61 gggtacggcc ctggccgcgg tggccgaaca ggcgcccgcc tcacccccgg cgcgggattg
      121 gaccgaggcc cacagggcct acctcgactc cagcacgata cccccggatc gcgccaggga
      181 aggcatcgat cccgcgcggg tagtcggcgc catgcgcgaa gccctgcccg aggacgctct
      241 attgaccaac gacgcgggca acttctcggt cttcatccac cgttactggc gctacaacca
      301 cgccggtacg cagctggcct ccactaacgg ggcgatgggc tacggtgtcc ccgctgcggt
      361 ggcggcgaag ctcgccgcgc ccgaccggac cgtcgtggcc tgctgcggcg acggcgggtt
      421 cctgatgacg ggccaggaga tcgagactgc ggtccgctac ggcgccgcta tcacggtggt
      481 cgtcttccgc aatcgactac acggcaccat agcgatgcac cagaagcagg cactcggccg
      541 tacggccggc gtcgagatag gagaggtgga tctcgccggt tacgccagga gcttcggcgc
      601 cgagggccac accgtgcgag aaccggaagg actggcgccc gccatggagg ccgctctagc
      661 ctccgacacc gttaccctgc tcgacgttgt caccgacccc gacattatca gcccttcgac
      721 aacgctctcc gagatcgccg gcgggagaac ctgacaacgc gagttgcgga atgcctttcc
      781 gcaactcgct gtgagaacgc gcggtgtgcg cggctctgtt ctctttgcgc caaagagttc
      841 tttggttact ctcgaggcat cgattcagtc tggtagacct ctcgcaggta tcgctgtaga
      901 cgtcgggctc gccgtcgatg aggtctacta cgcacacgtc gcatgatgtc cacctcctct
      961 ggctcagggg cagcgcggta caatcaggcc agctatcggc caggaggagg cgaaggcccg
     1021 tggatttctt gaggaagcga ctgcccgccc aggtgggtag gtacctggag ggtgtgaagt
     1081 tccccgcccg caaggaggag ctactcggaa ggctcgagcg caacggtgtg cccggcccgg
     1141 tcatcggcca gctgcgcaag aggctcccgg aaggcgagta ccgcggcccc caagacgtct
     1201 tgtcggccct gcgacagaga cgttagccgc gtttgctgcg cctggctcga cctttccgcg
     1261 ggcggaacct ccgagggccg gaagttcgta cgaggggtag cgagcacgat gaggcgttta
     1321 cgcgtaacct gtcgggcagc gagcgatact aggaggtacg agtcttggcg agcgagtcac
     1381 cagagaggct ggaggaattg cgcgagagat tcctacggac gctcagcgac ctgggcggcg
     1441 gggccgagga aggaagaccg gcaatgatct ctatggtcgc cgaaggcgcg ggcctggacc
     1501 cggaagggaa tccggaggag cgggcactga gcgagaggct ggccgcggag ctggtagacg
     1561 tagggtacgc ctcggcggag gcgggttcga gcggcttctt ggccataaca cccgaaggag
     1621 agcaggcgct caggggcggc accagctaag tcggcacgcc gcgggtaaga tgtttccgcg
     1681 ctgcggtgtt tttcgggcaa taggaagggc cagggaaagg aatccctggc cgcgtccatg
     1741 cacgaggggc catcaaatac gagccgtggc ggccctatct ttggccgaca gaggacctcc
     1801 tcaatcggtg tcttccttat cgcccggccc atcatcggcg tcttctttag cgtcttggcc
     1861 atcatcggcg gcttcgtcgc gcccgtctgg ctcgtcggct tcatcttcga gatcctggtg
     1921 gagtaccttg ccgtcgccgg cgtccacggt cacttcgtgc tccttgccgt ccttgcccgc
     1981 gatctcgatg tcatagacca ggtagccgtt atcgctcgtt tctagctccg tctcgtgcac
     2041 cgtgcccggc gcagcgtcga gtgcggcccg ctcggcggcg gcttcgtcta tcttggccag
     2101 ctcttgcgca gaagactcgt ttttgcctgc aacggagacg ctgcccttgt aggactcatc
     2161 tgcggcctcc tccgaggagt tgttgttggc ggcataggct acgccggcgc cgacgagcac
     2221 cacaaggacg accgccgctg ccaaaagcac cttcttcacg tacactttcc gtaactcctt
     2281 ttctttggtt ggctctcggt cggagtgtag tgggcgaaga tgtgaattgg atgtgaacgg
     2341 gatgagatat tcgacatctt ccacgccccc gggcgaatac gaaaggagga ccggacataa
     2401 ctctccagtc ctccctgtta tgttgttacg cggccgaacg ggccttctgt tggggctaga
     2461 gccagggcct agaactgcgg ttgcggcatc tcactcagct tcctctcgca cctgtgcaac
     2521 acgtcgatgc ggtcgacgta gacccgatcg aagtcgctgc cgcagaatat ctggtctttg
     2581 aagccgtcct tcgccgcaac cacgtcatcg ccagaacccg tgcctatgta gtcggtcttg
     2641 atgccgcctt gcatgtagtc gtcgttgcct ccgccgtaga gtttatcctc accgttgccg
     2701 ccgtagatgg cgtcctcccc acgattgccg ttgagggtgt ctttggcgcc gttcccttct
     2761 atttggtcgg caccgtctcg gccctctatc gcattgcggt tggacgtacc ggtcagttga
     2821 tcgtcgcggt cggtgccgct gcagttggta ttacaggtct tactcacccc gttctgggcc
     2881 agggccaccc ctgcggctag tagcagagct agcatcatcg acgctatcaa cagcataatt
     2941 ctcttcatac ctgcctcctg tccggacgcg agagcaagag cctctcccgc cttcgatgca
     3001 ccttccaagg tagacagctt accttctttg gagtgattga ggcaactcct tacggagtgg
     3061 tttggggacc tcttgacgag ctcttgcgcg agacaccttt cgaagccctc cgaaaagggt
     3121 tcgagcaacg cacctagcat ggatttgatt agtggttggt agaaatcccc cgtttcgggc
     3181 gcgtgatcgg gatcatagcc atccataaat ccctcgggta ccttattcct cgtgggtgac
     3241 aggcagctga tcagagctaa cggagtcagc aggaggatcg cgacggtagt catattggcc
     3301 accgtcgcag cctttctcat cgcggggggc tgcactacgg aaccggtccc ctccgcgcag
     3361 gaggtcgtcg cggcgggcga catagccagc tgccgcaccc aggccgacga ggctaccgcc
     3421 gagctggtcg agggtatcga cgggacggtc ctggctctgg gcgatgtggc gtatccccga
     3481 ggctcggcgg cgaacttcga ggaatgctac gggccgtcgt ggggacggtt caaggagcgc
     3541 accaggcccg tccccggcaa ccacgagtac ttcaccgaag gggccacggg ctacttcgag
     3601 tacttcggcg aggccgcggg cgaccccgag gagggctact actcctacga gctcgggtcc
     3661 tggcacgtgg tggccctcaa cagcaactgc gaggaggttc gctgcggccc gggttctccc
     3721 cagacgaggt ggctcgagga ggacctcgcc gccaacgagg aggcgcggtg caccctcgct
     3781 tatatgcacc atccacgctt cagctccggg gagaaacatg gctcctctga cgggggggtg
     3841 gagaacctgt gggaggcgct atacgaggcg gatgccgacg tggtcttctc cggccacgag
     3901 cacaactacg agcgtttttc cccgcaagac ccccagggga gggtagaccc cgagaggggc
     3961 atccgccagt ttgtagtggg cacaggcggt ggcggaggcg aggggccgat ctcggagccc
     4021 atcgtcaaca gcgaggtgcg catcgacggc atcgacggcg tcctcaagct caccctgcgt
     4081 ccgaagagct acgagtggga gtttgtcccc gtggagggtg agagctttac cgactccgga
     4141 tctgcccagt gccactgagc atcgattaga ggagggtcag ggctactaca cggctcgact
     4201 ccacttgcgg aatgtccccg ccgctcggcc agtcctcgtc ccccagacta gccgaccttc
     4261 gtgccagccc taccgacttt ctcacaaccc cctgcgacgt tgtcccccgg gttcgctttg
     4321 acgcgatcgg aaccagggcc acagtagatc tcgtccttgc tcccatccac cgcaaagatc
     4381 tggtcgttgc cgcgcccccc gcgtcccctg tccttgccgc cgccgaggcg caaggtatcg
     4441 tagccgcccc ttgcgtcgat gcggtcgtcg ccaccgtagc cgtaaatagc gtcaccattg
     4501 gaggttcctg ccagggtgtt cggtctctcg tcgcccttga tggaagcccg cgagaagcgt
     4561 gcgtagtagc tctgggtgac cccgctgacc gtcttgaact ctcctccggt gtgcagcctg
     4621 gcttcctcca cgtacagcgc ccacaccagg ctgtagctgc ccgagtacgt cgggctccag
     4681 ttgttttcta gccgtccgtt aaaggagtag gcggctatgc cctttctggt ctggcattca
     4741 tcgttggggt cctggacctc caagcggcgt ccgcaatcgt cgttcgggtg gtcggcgacc
     4801 tcctggaagt gtccgccgac aaccagctgc ccgcccatca ccgtcaccgc ctgcaccgaa
     4861 cctgaggtgt cgcgcaccca actgcgattg ccgccggctc ccttatccaa ctcggccaga
     4921 aagtcgctgc ctcccgccgc caggtagagc ttcccatcgt ggttgaccac ctcgtggccg
     4981 tacgaatccc tggccgcttt ggttacccaa gaggcgtcgt ggacgccctc ggtgctcagc
     5041 ttgaccaggg ccttggccag cttgccattt accgcgtcgc acaggcacgc tgcccagatc
     5101 gtcttgccgt cacgctccag gtcgcggtag gctgcctcgt ggacgcggtc gtacaggctc
     5161 tcgtcaatgg tgatcttcgc ccggctccaa agtgggcgct tcttgctgcc gtcgaactca
     5221 aaagccgaga ggctatcccc tccaccgtat accctgccta gatcaggcgc ccccagaacc
     5281 gacttcagcg gaggcgcctc gaaccacttg atcacccgtc cggtcttgcc gtcgacccgc
     5341 acgaggttgt ttcccttgcc gctcggccca gagaagctgc ccgctatcag cacgtcctcg
     5401 ccgtagaggg ccatgtccca cacttcggag tccgtgccgc caagcttggg tgcgatgttc
     5461 ttgtactgct gtgtcttgga gtcgaagacg gccaggttcg agacgttggc caccacggtt
     5521 cccttgtgct tcttgatgcg ggtgaacgtt ccacccagcc aggtgttgtt gcctacctgc
     5581 tcgatagccc tgacgcgccc gtcgaccatc ggggtcttgt cgggtctctc cgagggtagg
     5641 gccagtatgc ctccgctggc aagaagaagg gcgaccgccg tcgacgttag caacaagacg
     5701 atccttctca tccccgaggc cttctctagc tgggaggaag caaggaccag gagcccaacc
     5761 cagttacgca accagcggaa aataacggac catctctgcg acaggcacat cggctagagc
     5821 cccccaccct aactccgtga cttactaata ctgcgccgta gcaggagatg cctcaggcgg
     5881 tggggacgct tcaggaggcg tcgcctcggg aggaggagac gcttcaggag gtggtggtgg
     5941 tgggggcgat tgctgagacg aagacgtctc ggatgacggg gacgactgct gttgagacgg
     6001 cggagacgca gcgggtgagc tggaagatga gtcttgagcc ggggcagatg atgaggcaga
     6061 tgatgcagcc gaagatgagt cttgagccgg ggcagatgat gagtcttgag ccgaagccga
     6121 agatgaggcg gaagacgagg ctggagataa gtcggaaacc gactcgagag atccctcagg
     6181 agctattcct ccaccgctct cggaacccga cgtatcaccg gcctcggaac ccgactcact
     6241 accggcctcg gaacccgact tactacccgt ctctgaatcc gacttactac cagtctcggg
     6301 aatcgcctcg ctaatggcct cgggaaggga ggtgccgata gggttcatgt gcgcgatgag
     6361 cggcttcatc ctgggcacga agtaaccgaa gtacaacaaa atgttcaggg ccaccgcgac
     6421 tacccctacg accagcacca cgatcggctt atttagtttc tgctctaacc tactcatttg
     6481 agcgggcatc ccccttcact agtctccgaa catgaaggat aacgcaacta acgaaacttc
     6541 gctagagaaa cgccatcgtt gcgacacttt ccccggagaa tgcatccacg cacccaggcg
     6601 agtgggtgca atagcagcga tcaaggccgc aagaacctgg ccggttgcgc tctgctagcg
     6661 cgaactccgt cctgggcgct ctctcgtacg cgcttccccg ggatccgtct ttcgcgtctc
     6721 gacagcaatc agtaccagag agtagtcgct ttgccccatt cgcatctggg cgaattagac
     6781 cagcacaaaa tggtcgcttt gtccgattcc ttcttcccta cccggcccta agatcacgac
     6841 aagctattgc ggcaaccccg aaggaggtcg aaggaggttt acttcgagcg atacccgcag
     6901 gaatgagcag caagcgtggc c
//
LOCUS       DATMVF010000135         8538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_295234, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000135 DATMVF010000000
VERSION     DATMVF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295234"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..519)
                     /locus_tag="VN178_06965"
     CDS             complement(28..519)
                     /locus_tag="VN178_06965"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWS80751.1"
                     /translation="MAIVGYMGSGKTTLGRHLARALRLEFVDLDRAIAKSAGKGIPEI
                     FAQHGEKHFRDLEHEALVRALDGETPSVVACGGGTVVRPDNRTLLRDVTTIFLREDTD
                     VLYQRTRGPGRPLRGASREEFGLRYAKRLPLYREVADIEVEADRRPPEEVAEEVLACL
                     SRR"
     gene            complement(535..1698)
                     /gene="aroC"
                     /locus_tag="VN178_06970"
     CDS             complement(535..1698)
                     /gene="aroC"
                     /locus_tag="VN178_06970"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWS80752.1"
                     /translation="MRFGFSTAGESHGPAEVALVHGVPAGLGLIAGDVNRDLSRRQKG
                     YGRGGRQKIERDEVEFLGGVRHGYTLGSPVAMLIRNRDYANWEHRMSAEPVEDPPQKI
                     TLPRPGHADLAGMQKYDFDDLRNVLERSSARETTARVAAGGVAKRLLAEFGVEIRSAV
                     YRIGSVAYPKEQAVLDADASDESEVRCPDPDVSERMKAEIDAARYARDALGGEFVVVA
                     RGCPAGLGSYVDWRDKLDARLAAAMMSINAIKGVEFGLGFELAARRSSEVQDEILQEN
                     GRLARNSNRLGGLEGGMTNGEPVVIAAAMKPISTIAKALRTVDLATGEPTRAFKERAD
                     SCAVPAAAVIGESMAAIVLAEAFLEKFGMDNMTDIRAAYDAYKERVESTGKRR"
     gene            complement(1948..2571)
                     /locus_tag="VN178_06975"
     CDS             complement(1948..2571)
                     /locus_tag="VN178_06975"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80753.1"
                     /translation="MDTFLRDNRVLPPILALLALFVFAWVLAGVFLGGTDDQKPVAHR
                     SEIAQTDGAGGSDPAAPEVANPNVDSYAAYRSKDPFRQIMAEGTTIPEGTASPPPEQT
                     TTGPGRQNGDRPANGGNRQPRDTDGDGLSDRKEATMSLDPNNPDTDGDGIPDGLDDSD
                     GDGIPDGVEGNAAGGGDNRAGGGGSGAGERPGRGGGLLDSGGTLTPP"
     gene            complement(2618..3328)
                     /gene="pilO"
                     /locus_tag="VN178_06980"
     CDS             complement(2618..3328)
                     /gene="pilO"
                     /locus_tag="VN178_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HWS80754.1"
                     /translation="MNRNDRNILILGILGIVIVVIGFYFLLLSPLLQSLNEQAEARED
                     KQAQLEQVQQEVNELEEVRRNSPEIQRQLLELSKRVPTQPQIPTLVVQVEEIADASGV
                     TQLSVDPEPPTAPAGGGDYQVVPVTMQFNGTYDQMQDFLLRTRNLTRLVTVTNLSYCR
                     IPTIGEEASCEVEGEASGTTDETTTLNPAVEPRLSVEVQADVYFQPEGVPSGAETTAP
                     TPQETTTPSPPAEVTGGG"
     gene            complement(3325..4086)
                     /locus_tag="VN178_06985"
     CDS             complement(3325..4086)
                     /locus_tag="VN178_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80755.1"
                     /translation="MRRINLLPPDERGRPADRLRSGVVGILLVVGAFVLILMVGVYLV
                     LLLRLGDLEDQVAQLDAEIARQNAALAELSPYRDLKARLEAKKPVADGIFRSRFAWDQ
                     FLQGLAFVVPESTALDTFTAEAAPVDLDAPVEQPLDPPGTITFTGIALPRYENVADFV
                     VRMDNLQYLANADLELATGPSNSRVSPVSFEVVSELVTLVGENGTEIRLEGGSQDELA
                     GRQGPLRTREIANMKRPSYLVAGEQDAGHTERGRR"
     gene            complement(4083..5192)
                     /gene="pilM"
                     /locus_tag="VN178_06990"
     CDS             complement(4083..5192)
                     /gene="pilM"
                     /locus_tag="VN178_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HWS80756.1"
                     /translation="MARLPGFGGSRTVGLDIDRGSIKAVQISGAGGGYSLQHVGYHRL
                     PPGTILDGEVADHDLLAAEIREFWDSHSFTGKAVTLGVSNQKVVVRLLDFPHMAQADL
                     QGAISFEAQDHIPMPLDEAVLDYVVLGPTEERSDLDRVLVVAAQREMISRYTSAVRTG
                     GLRPAGVDVKALSLTRSTLPDPFFGDEGAVLLLDVGAEITNLVVADRGNPVLTRFVPV
                     GFVDFVAAVVELADLLEDEAERLALDPRVRLGSEQDEIQETEEGSLDPALVFDVRRGL
                     EGAAQTLADEVQRSIEHHRSQESARDVSRVLLSGEAALIPNLDGYLGELLDVPTSPGK
                     PVEKLSANRSNVTDDQLRAMEPVLAVAFGLAMEEE"
     gene            complement(5233..5868)
                     /locus_tag="VN178_06995"
     CDS             complement(5233..5868)
                     /locus_tag="VN178_06995"
                     /inference="COORDINATES: protein motif:HMM:NF036822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80757.1"
                     /translation="MPEPEVEVQRLAFDEELRVAIVGAGGYGRVALDVLLAGGLGESV
                     LGFYDDAHAAISEKVRGYPVLGDIEMLKSMLSVEPVHVVVAITDNTSRLRVANSLRAL
                     GGQFFTAIHPLAYVSGVAAIGGGCVVTAGVAVHPDTAVGSHCFLGSGSVVDRDAEVGA
                     GSWISAGAVVGSGARVGARAVLGPNSSVGRKASVGADAEVGALSHVAREDV"
     gene            complement(6026..6943)
                     /gene="fmt"
                     /locus_tag="VN178_07000"
     CDS             complement(6026..6943)
                     /gene="fmt"
                     /locus_tag="VN178_07000"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWS80758.1"
                     /translation="MKIAFAGTPHFAATILRGLISSPHEVGLVISQPDTRKGRGRKTG
                     RTPVAELASTHDLPLRQPASISEVSAELSNYDALIVAAYGQILRPETLYAASKGAYNV
                     HASLLPKYRGAAPVERAIMAGETRTGVTIIRMDEGLDTGPIALQRPAAIPPDMNAGEL
                     TDLLAQTGARAIVESLNLLQANTLTLVDQESRHATYASKLSNRDKTIGWSKEAGEVHN
                     QIRALAPHIGARTFHPDIEGPVKIWRSAVLDRAAASSGAGSIAVANGRFVVCCGKGRL
                     DVLELQVPGGRRLPARDFLLGNSLEGAFR"
     gene            complement(6953..7450)
                     /gene="def"
                     /locus_tag="VN178_07005"
     CDS             complement(6953..7450)
                     /gene="def"
                     /locus_tag="VN178_07005"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWS80759.1"
                     /translation="MALDIRKFGDPVLKTRAAPVESFDESLVRLTQEMLTTMRDNEGV
                     GLAATQVGRLKRVFVAAIEDDEYVIVNPVLTHRSQTTETATEGCLSIPGIHVDVERPT
                     SVTISGQDVSGEPLLIEASDLLARVLQHEVDHLDGVLILDRTDRQSRKAAMREWREQL
                     LAQQG"
     gene            complement(7451..>8538)
                     /locus_tag="VN178_07010"
     CDS             complement(7451..>8538)
                     /locus_tag="VN178_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80760.1"
                     /translation="EGLPLHVRDVALERARLDRASVIFLSPFPSLRLYAAEVRRRERI
                     RELPTAKNGPWPAVRIVDMRGSGATFSSTLMDACRRCMEGGGRTGVVLKRLGYATSVS
                     CSRCGTVKICPNCDLPLVSQKREASLDCTRCGYSEEPGPCVECGTRRMRQTGLGVERV
                     REELSEVLGARVGLITAEDQDNADALIVVGTAPRILEGEWDAVILPDADAFLAANGIG
                     AVERAFRLFYGSAEAARKLLLIQTRVPEHYALRAAVRGDYEAFASAELPRLRALGYPP
                     FAYAASLTFEGSEAALFGAVESRLRQALEAGVEMSGPVLLGHTAWRVLLRAKRRSVVA
                     RAATLAARLAARTRGLEVRVDVDPEEV"
BASE COUNT         1482 a         2796 c         2651 g         1609 t
ORIGIN      
        1 cgctgtacgg atgggtgacg gggaccgcta ccgtctcgac aggcaagcca gaacctcctc
       61 ggccacctct tccggcgggc gtctatcggc ctcgacctcg atgtctgcga cctccctgta
      121 gagggggagg cgtttcgcgt agcgcagccc gaactcttcg cggcttgccc cccgcaaggg
      181 gcggccagga ccacgagtcc tctggtagag aacgtccgtg tcttccctca agaagatcgt
      241 agtcacgtcg cgcaggagcg ttctgttgtc gggacggact acggtaccac cgccgcaggc
      301 gaccacgctc ggagtctcgc cgtcgagggc tcgaaccagg gcttcgtgct ccagatccct
      361 gaaatgcttc tcgccgtgct gggcgaagat ctccggtatc cctttgcccg cgctcttcgc
      421 gatggcacgg tcgaggtcca cgaactcgag tctcagggca cgggccaggt gccggcccag
      481 ggttgtcttg ccgctaccca tgtacccgac gatggccaca gatccttcca agcttcagcg
      541 tctcttcccc gtgctctcga cacgttcctt gtaggcgtcg taggcggcgc ggatgtcggt
      601 catgttgtcc ataccgaact tctcgaggaa cgcttccgcg agcacgatcg cggccatgga
      661 ttcccctatc acggctgcgg ccggtaccgc gcaggagtcg gcgcgctcct tgaaagcccg
      721 agtgggttct cccgtagcca ggtcgaccgt acggagggcc ttggcgatag tggagatggg
      781 cttcatcgcc gcggcgatca cgaccggctc cccgttcgtc atcccgcctt cgagtcctcc
      841 tagtcggttg ctgtttcggg cgagacgtcc gttctcctgg agtatctcgt cctgcacctc
      901 actggacctt ctggccgcga gctcgaagcc cagcccgaac tccacgcctt tgatcgcatt
      961 gatcgacatc attgccgcgg cgagcctggc atccagcttg tcgcgccagt cgacgtagga
     1021 gccgaggccc gccggacaac ccctggcgac gacgacgaac tcccccccca gggcgtcgcg
     1081 ggcgtagcgg gcggcgtcga tctccgcctt catcctctcc gagacatcag ggtcgggaca
     1141 gcgcacctcg gactcgtcag aggcgtcggc gtcgaggacg gcctgttcct tgggataagc
     1201 gacagatcca attcggtaga ccgcgctccg gatctcgacc ccgaactcgg ccagaagcct
     1261 cttcgccacg ccgcctgctg ccacgcgggc cgtagtctcg cgggcgctgg agcgttcgag
     1321 gacgtttcgc aggtcgtcga agtcgtactt ctgcatcccg gcaaggtcgg cgtgacccgg
     1381 cctgggcagc gtgatcttct gcggtgggtc ctcgaccggt tctgcgctca tgcgatgttc
     1441 ccagttggcg taatccctgt tgcggatgag catggcgacg ggcgagccca gggtgtagcc
     1501 gtggcgcaca ccgcccagaa actcgacctc gtctctctct atcttctggc gcccaccccg
     1561 cccgtaaccc ttctgcctgc gggaaaggtc cctgttcacg tcccctgcga tgagcccaag
     1621 acccgcaggg accccatgca ccagggctac ctcggcgggg ccgtgggatt ctcccgccgt
     1681 agaaaaaccg aacctcacaa acgcctccga gctctcttga ccgccaagct tttgacgcca
     1741 atatgctagc atttcagcac gctcagcatt tcagcacgct caggctttca gccttcgctc
     1801 gtcagcactt cagcccggcg gctacgccgc cttgtcagcg agtcagcaag gacttgcgcc
     1861 cgcgacaagc ttacaagtcg cctgttccag gcgacgtgct gacgagcgaa gcccgaaggg
     1921 cgtagcgtgc ttacatgcta gagtgctcta cggtggcgtg agagtcccac cgctgtcgag
     1981 cagaccaccg ccccgtcccg gccgctctcc tgcgccactc ccgcccccgc ctgccctgtt
     2041 gtctccgccc cccgcggcat taccctccac cccgtcaggg ataccgtcgc cgtcagagtc
     2101 gtcgaggccg tcagggatac cgtcgccgtc ggtgtcgggg ttgttcggat cgagactcat
     2161 agtcgcctcc ttgcggtcgg acaggccatc accgtccgta tccctgggct gtctattgcc
     2221 gccgttggca gggcgatcgc cgttttgcct ccctgggcct gtggtggtct gctcaggggg
     2281 aggggaggca gtaccttccg ggatcgtcgt accttcggcc atgatctgcc tgaacggatc
     2341 cttactgcgg taggctgcgt aagaatccac gttggggttg gctacctcag gagcagcagg
     2401 atcagaaccc cctgcgccgt ccgtctgggc gatctcggac ctgtgcgcga ccggcttctg
     2461 gtcgtcggtc ccgccgagga atacgccagc aaggacccag gcgaagacga acaacgcgag
     2521 tagggccaga atcggcggca gcaccctgtt gtcacgcaag aacgtatcca caagacctct
     2581 cgcgaggtcg gccacgcggt gtgggaccca ccaccgccta gccaccgcca gtaacctcgg
     2641 cgggaggtga cggggtggta gtctcctgag gggtcggcgc cgtggtctca gcacccgaag
     2701 gcacgccctc cggctggaag tagacgtctg cctgtacctc caccgacaga cgcggctcca
     2761 cggcaggatt caacgtcgtg gtctcatccg tagtccctga cgcctcgccc tcgacttcgc
     2821 acgaagcctc ttcgccaatg gtagggatgc gacagtaact caaatttgtc accgtcacta
     2881 acctggtcag gttacgtgtt ctcaacagaa aatcctgcat ctgatcgtag gtcccattga
     2941 attgcattgt aacggggacg acctggtaat ctccaccacc agcgggagcg gttggaggtt
     3001 ctgggtcaac cgagagctgg gtcacaccgg aggcgtccgc tatctcctcg acctggacga
     3061 cgagcgtagg aatctggggc tgcgtgggta ctctcttgct gagttcgagg agctggcgct
     3121 ggatctcagg ggagttgcgc ctgacttcct cgagctcgtt cacctcttgc tgtacctgct
     3181 cgagctgggc ctgtttgtct tccctcgcct cggcctgctc gttcagactt tgcagaagcg
     3241 gactcaaaag gaggaagtag aagccgatga cgacgatcac aattccaagt atcccgagga
     3301 tgagtatgtt acgatcatta cgattcatcg gcgcccacgt tccgtgtgcc cagcgtcctg
     3361 ttcacctgcc accaggtagg agggacgctt catattggcg atttcgcggg tacgcagcgg
     3421 accctgtctg cccgcgagct cgtcctgcga tccgccttcc agccttatct ccgtgccgtt
     3481 ctccccgacc agcgtgacca gttccgagac gacttcgaag ctgacagggg agactcgtga
     3541 gttcgatggc ccggtggcta actcgaggtc cgcattggcg agatactgga ggttgtccat
     3601 gcgcacgacg aagtctgcca cgttctcgta acggggtagt gcgatgcccg taaacgtgat
     3661 cgtgcccggg ggatccaggg gctgctcgac cggcgcgtcg aggtcgacgg gtgcggcctc
     3721 ggcggtaaag gtgtcgaggg ctgtgctctc tggcacgacg aaggccaggc cctgcaagaa
     3781 ctgatcccag gcgaagcggc tgcgaaagat gccgtcggcg accggcttct tggcttcgag
     3841 gcgcgccttg agatccctgt aggggctcaa ctcggccagg gctgcgttct ggcgcgcgat
     3901 ctcggcatcg agctgcgcga cctggtcttc gagatcaccc agcctcagaa gcagtaccag
     3961 gtacacgccg accatcagga tcaacacgaa ggcgccgacg acgaggagga tacccacaac
     4021 cccgctgcgt aacctgtctg ccgggcggcc acgctcgtcg ggaggaagga ggtttattcg
     4081 cctcattctt cctccatcgc gaggccgaac gcgaccgcga gcacaggctc catcgccctc
     4141 agctggtcgt cggtgacgtt ggagcggttg gccgagagct tctctacggg tttccctggg
     4201 ctggtcggga cgtccaacag ttccccgaga taaccatcca ggttggggat caaagccgct
     4261 tctccggaga gaaggaccct ggagacgtca cgggcggatt cctgggagcg gtggtgctcg
     4321 atggagcgct gcacctcgtc ggcgagggtc tgcgcggcgc cttcgagccc gcgcctcacg
     4381 tcgaagacta gcgccggatc gagactacct tcctctgttt cctggatctc gtcctgctcg
     4441 gatcccagcc tgacccgcgg gtcgagcgcc agcctctcgg cttcatcctc gagcaggtcg
     4501 gcgagctcga ctacggcggc cacgaagtcg acgaagccga cgggcacgaa gcgggtaagg
     4561 accggattac cccgatccgc caccacgagg ttcgtgatct ccgccccgac gtcgaggagg
     4621 agtacggctc cttcatcgcc gaaaaagggg tcggggaggg tagagcgcgt caacgagagt
     4681 gctttgacat ccacgccggc gggccgcagt ccaccggtcc ggaccgccga ggtgtaacgc
     4741 gagatcatct ccctctgggc ggcgaccaca agaacgcggt cgagatccga gcgctcctcc
     4801 gtggggccga gcacgacgta gtccagaacg gcctcgtcga gcggcatcgg tatgtggtcc
     4861 tgggcctcga aacttatggc gccctgcaag tcggcttggg ccatgtgcgg aaagtcgagc
     4921 agcctgacca cgaccttctg gttggagacc ccgagggtga cggccttgcc ggtgaacgaa
     4981 tgagaatccc agaactccct gatctcggcg gccaggaggt cgtggtcggc gacctctccg
     5041 tctagaatcg tccccggcgg aagcctgtga tagccgacgt gctgcaggga gtatccgccg
     5101 ccagcgccgg agatctgaac ggccttgatg gacccgcggt ctatgtcgag cccgacggtt
     5161 cttgaccccc cgaatcccgg taacctcgcc actacgccaa actccggaag atcgcgaaga
     5221 tggaccgtgc gatcaaacgt cctccctggc cacgtggctg agcgccccca cctcagcgtc
     5281 ggcccccacg gaggctttgc gtccgacgct ggagttggga ccgaggaccg ccctggcccc
     5341 gactcgggcc cctgacccta cgaccgctcc cgcggagatc caactcccag cccccacctc
     5401 cgcgtcccta tccactacgg aaccggatcc caggaaacag tggctcccca ctgccgtatc
     5461 tggatgcacg gccacgcccg ccgtgacaac gcaaccgccc ccgatcgcgg cgacgcccga
     5521 aacgtatgcc agcggatgga tggccgtgaa gaactgccct ccaagggccc tcaatgagtt
     5581 ggctacccgc aacctggagg tgttgtccgt gatagccact acaacgtgca ccggctcgac
     5641 tgagagcatg ctcttgagca tctcgatgtc cccaaggacc ggataccctc tcaccttctc
     5701 cgagatcgcc gcgtgggcgt cgtcgtaaaa cccgagcacg ctttctccca acccacccgc
     5761 caagagaacg tctaacgcta cccttccgta gccgccagcc cctactatgg cgactctcaa
     5821 ctcttcatcg aaggccagtc gctgcacctc gacttcaggt tcaggcacgc cggtagcggt
     5881 ttcgggctct cttatctccc tctccctcca acagagatca ctccaagcgc cctaggaagt
     5941 ttagcatcgt tgttggctag ttgctcctaa acaccacatc atcatgcgct cgcccatcgc
     6001 gactatgcta ggtgtagggg acttgctatc gaaacgcacc ttcgagagag ttaccgagca
     6061 ggaaatcacg cgccgggagt cgtcggccac ctggtacctg gagttccagc acgtcgaggc
     6121 gaccttttcc acagcacact acgaaacgcc cgtttgcgac ggcgatggat cccgccccgg
     6181 aagacgcggc cgcacggtcg agcaccgcgg aacgccagat cttgaccggt ccctcgatat
     6241 ccggatggaa cgtcctcgcc cctatgtgcg gggccagggc tctaatctgg ttgtgcacct
     6301 cgccagcctc cttgctccac cctatggtct tgtcccgatt cgaaagtttt gaagcataag
     6361 tagcatgacg actttcttgg tcgacaaggg tcagggtatt tgcctggaga agattcaaac
     6421 tctctactat agctctagcc ccggtttggg cgagaaggtc ggtcagttct cccgcgttca
     6481 tatcgggagg tatagctgcg ggacgctgga gtgctatggg gcccgtatcg agaccttcgt
     6541 ccatgcggat gatggtcaca ccggtcctgg tctccccggc cataatggcg cgttcgacgg
     6601 gggcagcgcc gcggtatttc ggcaggagcg aggcgtgtac gttgtaggcg cctttggagg
     6661 cggcgtagag ggtttcggga cgcaggatct gtccgtaggc tgcgacgatc agggcgtcgt
     6721 aattcgagag ttcggcgcta acctcggaga tgctggccgg ttggcgaagg gggaggtcgt
     6781 gggtgctggc aagttcggcg acgggggttc tcccggtctt gcgtcccctg ccctttcggg
     6841 tgtctggctg ggagatcacg agccccacct cgtgggggga ggagatcaga ccccgaagga
     6901 tggtcgccgc gaagtgtggg gtaccggcga aggcgatctt caaggtgtct tttcagccct
     6961 gttgggcgag cagttgctcc cgccactcgc gcatggctgc cttgcgagat tgcctgtcgg
     7021 tgcggtccag aataaggacg ccgtcgaggt ggtcgacctc gtgctgcaag accctggcta
     7081 gcaggtcgga ggcctcaatt agcagcggct cgcccgagac gtcctggccc gagatggtta
     7141 cgctagtagg acgttcgacg tccacatgga tccccggtat ggagaggcat ccttcggtgg
     7201 cggtctccgt cgtttgagac ctatgcgtaa gaaccgggtt gacgatcacg tactcgtcgt
     7261 cttcgatggc ggcgacgaac acccgcttga ggcgtccgac ctgggtggct gccaggccca
     7321 ccccctcgtt gtcgcgcatg gtggtaagca tctcctgggt aaggcggacc agcgactcgt
     7381 cgaagctctc gaccggcgcg gcgcgggtct tgagcacggg gtctccgaat ttccttatat
     7441 ctagtgccac ctatacctcc tcgggatcca cgtcaacgcg aacctcgagc ccgcgcgtgc
     7501 gggctgctag cctggcggcc agggtcgccg cgcgggccac gacagaccgc ctcttggccc
     7561 tgagcagcac acgccacgcg gtgtgcccga gtagaacagg gcccgacatt tcgaccccgg
     7621 cctcgagtgc ctgccgcaaa cgggattcta ccgcaccgaa gagagctgcc tcggaaccct
     7681 cgaaggtcag agaggcggcg taggcgaacg gagggtagcc gagcgcgcgg agccttggga
     7741 gctcggcgga ggcgaacgcc tcgtagtcac cccttactgc ggcgcgcagc gcgtaatgct
     7801 cggggacgcg cgtctggatg aggagcagct ttctggccgc ctccgccgag ccgtagaaca
     7861 aacggaaagc tctctccacg gcgccgatgc cgtttgctgc aagaaacgcg tcggcgtcgg
     7921 gcaggatcac cgcgtcccac tcgccttcca ggatgcgggg cgcggtccct accacgatga
     7981 gtgcgtcggc gttatcctgg tcttcggcgg tgatgaggcc gacccgcgct ccgaggacct
     8041 cggagagctc ttcacgcacc cgctcgacac cgagacccgt ctgccgcatg cgcctggtac
     8101 cgcactcgac acacgggccc ggctcctctg aatatccgca gcgggtgcag tctagagaag
     8161 cttcccgctt ttgcgagacg agtggcaggt cgcagttcgg gcagatcttt accgtcccgc
     8221 agcggctgca tgagaccgaa gtcgcataac ccagccgttt gaggacgacg cccgttctac
     8281 cccctccttc catgcagcgg cggcaggcgt ccatgagggt ggagctgaac gtcgcgccag
     8341 agccgcgcat gtctacgatc cggacggcag gccacggacc gttcttggcc gtgggaagtt
     8401 cgcgtatgcg ctctcgcctg cggacctcgg cggcgtagag cctgagcgat gggaaaggcg
     8461 agaggaagat taccgatgcc cggtcgaggc gagcacgctc cagcgccacg tcccgcacgt
     8521 gcaggggaag accttcgt
//
LOCUS       DATMVF010000136         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_427452, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000136 DATMVF010000000
VERSION     DATMVF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427452"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VN178_07015"
     CDS             <1..412
                     /locus_tag="VN178_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80761.1"
                     /translation="EGREGTGTVFVLTSSGLRHMGLRPGPGRPNATGRGFLGYLQEIF
                     WKDALELVHEDDLPLLWFLLSLVKEEPGASESVEVRFRDAWEGWILMDVCVLNVLEAP
                     TGHDTGLLVVNVRQTRESTSFSAGTRNGSLSTPR"
     gene            660..2039
                     /locus_tag="VN178_07020"
     CDS             660..2039
                     /locus_tag="VN178_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWS80762.1"
                     /translation="METVRGWTDGDLRRLDEIIVEGERRFKERQPRSGALIEEARGVL
                     AGGATSSWQIARPQMIWVSYGEGSKIYDADGGEYVDLHGGYGVGAVGHGHPKIVEALT
                     RRVPRGTHFAQPTEDAIVVADELARRFGLPLWRFSNSGTETTMDAVHLMRAITGRDLI
                     VKVEGCYHGHHDSVMVSVSNEKDEIGPAERPYSAPSGTGIPRAITDLTLIVPFNDLDA
                     LERLFEEHRGQIAGMILEPIMMNAGVIPPEQGYLEGVREIVHEYGALLAFDEVKCGVT
                     TSYRGASGYLGVSPDMVCLAKAIGGGVPCGAIGGTEEVMGRIADGTYDQVGTFNGNPL
                     TMAASRATLTEILTPEAYEHFDRLAKTMSEGVQDVVDEYDLAAYPRAFGAKGSVTFSG
                     RNVRNYRDFLELDDRFSQAHWLFQHNGGVFLPPWGKAEQWTLSVQHSEDDARRFVDNF
                     ESFARALRR"
     gene            2036..3202
                     /locus_tag="VN178_07025"
     CDS             2036..3202
                     /locus_tag="VN178_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS80763.1"
                     /translation="MNAEEGPAGGHVQLVDLAKRFGAFEAVRGIDLEIPPGEFFSLLG
                     PSGCGKTTTLRMIAGFERPSGGHILLDGKDVSDTPPNRRNVNTVFQSYALFSHLTVAE
                     NVAFGLRFAKASREETRSRVGEALALVRMQEFRDRKPHQLSGGQQQRVALARALILNP
                     SVLLLDEPLGALDAKLRKALQIELKALQEQVGITFVYVTHDQEEALTMADRLAVMNEG
                     RIEQAGSPREVYEEPATAYVADFLGVSNLLDAYATGTDSEGRCRVRVGDSELLASCGH
                     TSARGSVKVVVRPERVRVEAPQKTGENRLPGRIERVVYAGAISQLVVTLDRGTPIRCM
                     LANDGAASSFDRGAPVSVCLPSEALRVLRTEHTGANEEESGEPSITSARTNSVV"
     gene            complement(3383..>4057)
                     /locus_tag="VN178_07030"
     CDS             complement(3383..>4057)
                     /locus_tag="VN178_07030"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80764.1"
                     /translation="PSLRAALVQSLELAALTMLIATPLGVALAIGLARWHGWGSRPAN
                     SLMLFPLVTPEIVMGVALFLVFVHLYGFVRLGTMAQLLGHVTFTMSYIVIVVRGRLFS
                     IGKEHEEAAMDLGASRWQAIRMVLLPMLAPAIWASLMVAFAISIDDFVISAFLMGDAS
                     STTIPVKLYSAARLAPSPALNALASILLFASMLAIAIAILVMRRSRKKEGAEGSAVED
                     FARLDL"
BASE COUNT          779 a         1229 c         1342 g          707 t
ORIGIN      
        1 ggaaggacgg gaaggaaccg gcacagtgtt cgtcctgact tcctcaggcc tgcgccacat
       61 gggcctccgg cccgggccgg gccgcccgaa cgccacaggt agagggtttc tcggctatct
      121 tcaggagatc ttctggaaag acgccctcga gctggtccac gaagacgacc tgcccctcct
      181 ctggtttctc ctctccctgg tcaaggaaga gccgggtgcc tcggagagcg tcgaagtccg
      241 tttccgcgac gcgtgggaag gttggatcct catggacgtc tgtgtcctga acgtgctcga
      301 ggcccccaca ggtcacgaca caggactact cgtcgtaaac gtgcgccaga cgagagagag
      361 tacatctttt agcgccggaa cgcgcaacgg gagcctgtcg actccccgct aagcttcagc
      421 atttcagcac gctcggacct tgcggccctc gcttgtcagc acggcggctg cgccgccttg
      481 tcagcatgtc agcaagatcc tgcccatacg aaactaacaa gctgaaatgc tgacaagcgc
      541 ccgacagggc gcgtgctgaa gtgctgacaa gcctgcgaag caggcgtgct gaaatactga
      601 agcgttgaca tagacgagcc atacctctat tattgaatca ctgtttaaag gaggagtgta
      661 tggagactgt gcggggttgg acggatgggg acctccgcag gctagatgag atcatcgtcg
      721 agggtgaacg ccgattcaag gaacgccagc ccaggtcggg ggctttgatc gaggaggcgc
      781 ggggggttct ggctgggggg gcgacctcga gctggcagat cgcccgtccg cagatgatct
      841 gggtcagcta cggggaagga tcgaagatct acgacgccga cggtggggag tatgtggatc
      901 tccacggcgg gtacggcgtc ggggccgtcg gacacggcca tccgaagatc gtcgaggcct
      961 tgacgcgcag ggttccgcgg gggacgcact tcgcccaacc gacggaggac gctatcgtcg
     1021 tggctgacga gctcgcgcgg cgtttcggtc ttccgctgtg gcggttctcc aactcgggca
     1081 ccgagacgac gatggatgct gtgcatctca tgcgtgcgat cacgggccgg gacctgatcg
     1141 tcaaggttga gggttgttat cacggacacc acgattcggt catggtctcg gtgtcgaacg
     1201 agaaggatga gatcggaccc gccgaacgtc cctacagcgc gccttccggg acgggcatcc
     1261 cgcgggctat caccgacctg accctaatcg tccctttcaa cgacctcgat gccctggagc
     1321 gactcttcga ggagcatcgc ggacagatag cgggcatgat cctcgaaccc ataatgatga
     1381 acgccggcgt tattcccccc gagcagggct atctggaggg cgtgcgcgag atagtgcacg
     1441 agtacggagc cctgctcgct ttcgacgagg tcaagtgtgg cgtcaccact tcctacaggg
     1501 gagccagcgg gtacctcggc gtaagccctg atatggtgtg cctcgccaag gcgataggtg
     1561 gtggcgtccc ctgcggggcc atcggcggga ccgaggaggt catgggccgc atcgccgacg
     1621 gcacctacga tcaggtcggc accttcaacg gcaacccgct tacgatggcc gcctcccgcg
     1681 ccacgctgac cgagatcctc acccctgagg cttacgagca cttcgaccgt ctcgccaaga
     1741 cgatgtccga aggggttcag gatgtcgtgg atgagtacga cctcgccgcc tacccgcggg
     1801 cgttcggggc caaagggtcc gtgacgttct caggcaggaa tgtccgaaac tacagggatt
     1861 tcctggagct cgacgaccgt ttcagccagg cgcactggct cttccagcac aacggcggtg
     1921 tcttcttacc gccgtggggg aaagcggagc agtggacgct ttcggtgcag cactccgagg
     1981 acgatgccag gcgcttcgtg gacaacttcg agtccttcgc gcgggcgtta cgcaggtgaa
     2041 cgcggaagaa ggccccgcgg gggggcatgt acagctcgta gatctcgcca agcgctttgg
     2101 ggccttcgaa gccgtgcgcg gcatagacct ggagatcccg ccgggagaat tcttctcgct
     2161 gctaggcccg agcggttgcg gcaagacgac gacgctgcgt atgatcgccg gtttcgagcg
     2221 tcccagcggt ggacacatct tgctcgacgg caaggacgtg tcggacaccc ctccgaaccg
     2281 gcgcaacgtc aacaccgtgt tccagagtta cgccctcttc tcccatctga cggtcgccga
     2341 gaacgtcgcc ttcgggttgc gtttcgcgaa ggcgtccagg gaggagactc gtagcagggt
     2401 cggagaggca ctcgccctcg tgcggatgca agagttccgc gaccgcaagc cgcatcagct
     2461 ctccggcggc cagcagcagc gggtggcact cgcgcgggcc ctcatcctca acccttcggt
     2521 gctattgctg gacgagccgc tcggcgcgct cgatgccaag ctcaggaagg cgctccagat
     2581 cgagctgaag gccctgcagg agcaggtcgg cataaccttc gtctacgtga cgcacgacca
     2641 ggaagaggcc ctcaccatgg ccgaccgcct ggcggtgatg aacgagggca ggatcgaaca
     2701 ggccggctct ccccgcgagg tctacgaaga accggcgacc gcctacgtcg ctgacttcct
     2761 cggcgtctcc aacctgctcg acgcgtacgc aacaggcacg gattccgaag gcaggtgccg
     2821 ggtcagggtc ggggactccg aactgcttgc ctcatgcgga catacctcgg ccaggggttc
     2881 cgtcaaagtt gtggtgcgtc cggaacgggt gcgtgtagag gctcctcaaa agaccggcga
     2941 gaaccggttg ccggggagga tcgaacgcgt cgtctacgct ggagccatct cacaactcgt
     3001 agtcaccctc gaccgcggga ccccgatccg gtgcatgctc gccaacgacg gcgcggcgtc
     3061 atccttcgat cggggagcgc ctgtgtcagt gtgcctcccg agcgaggccc tgcgtgtgct
     3121 gcgcaccgaa cacaccgggg cgaacgagga ggaaagcgga gagccctcta taacgtccgc
     3181 ccggacaaat tctgttgtct gatgcctgat atgtaatccg acaaagcgat tccggttgga
     3241 ggagtagagc tcctgcgcag tggattcccg ctttcgcggg aatgacggtc atgatgtgag
     3301 cgatggggca ggatttgcgc tcgtgcagct tgtacacggc ggggaaataa tcgtgtggac
     3361 tccgtatgat gcctgttgcc tttcacagat ccaggcgggc gaagtcctcg accgcggagc
     3421 cttctgcgcc ttccttcttg cgggaacggc gcatcaccag gatggcgatt gcaatagcaa
     3481 gcatggaggc aaagaggagt atgctggcca gggcgttcag ggccgggctt ggggccagcc
     3541 gcgccgcgct gtagagcttg accggtatgg tggtggacga ggcgtctccc ataaggaagg
     3601 cgctgatcac gaagtcgtct atggagatgg cgaaggcgac catgaggctc gcccagatgg
     3661 caggggccag catcggcagg agcaccatcc ttatggcctg ccaccttgaa gcgccgaggt
     3721 ccatggccgc ctcctcgtgc tctttaccga tcgagaacag ccgcccgcgc acgactatga
     3781 cgatgtaaga catggtgaag gtgacgtgcc cgaggagctg cgccatcgtc cccagccgca
     3841 cgaagccgta gaggtgcacg aacaccagaa agagcgcgac gcccatcact atctccggcg
     3901 tgacgagcgg gaagagcatc agggagttgg ccgggcggga gccccacccg tgccagcgcg
     3961 cgaggccgat ggccagcgcg acacctaagg gtgtggcgat cagcatcgtc agcgccgcca
     4021 gctcgaggct ctgcacgagc gcggcgcgca gcgaggg
//
LOCUS       DATMVF010000137         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_566367, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000137 DATMVF010000000
VERSION     DATMVF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566367"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..746)
                     /locus_tag="VN178_07035"
     CDS             complement(87..746)
                     /locus_tag="VN178_07035"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HWS80765.1"
                     /translation="MSIRDLIAILPEPLRQRSLTHPSVADPYESNGRLEFLGDSVLNS
                     RVAELVFHGYPELLEGDLTRIRAHLVRKSFLAMVGRSEGIEEIIESSVINDSVIADTV
                     EAIIGGAYLIDRDLVLRTLDEIFNPAEVDTDELRDWKTLLQETLQADGLRPTYRVISK
                     QGPAHRPSFTSRVSVDGKEVATGEGSSIKDSEQTAARAALEILGIRPVPRSTVEVHVS
                     A"
     gene            complement(755..2011)
                     /gene="fabF"
                     /locus_tag="VN178_07040"
     CDS             complement(755..2011)
                     /gene="fabF"
                     /locus_tag="VN178_07040"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWS80766.1"
                     /translation="MTDPDGGGRGRALVTGTGAVTPLGTGVENFWDAALHGKSGIGEI
                     TLFDPAPYPSRIAGECWDFDPTDFIKKKQARRMDRFAQLGIAAGIQAAESAGIVDLIR
                     ERPERVAIVMGSGIGGLSTWEREYRVMNERGPGRVSPFLITMMVPNMAAGQLAIALGA
                     TGPSQCPVLACATGGEAIAEGLELIRRGDADVVIAGSSEAPITPLSMAGFCAIRAVSR
                     RNEEPARASRPFDEGRDGFVMSEGAGALILESEEHAERRGAEPVAAVAGYGRTTDAYH
                     VTAPDEDGRGVERAMVHAFEDSGLDAEEVGHVNAHATATPTGDGPETKAIARQVPHAL
                     VSGTKSMTGHSFGAVGATEGIMCALAIKHKVVPPTINLENLAEDCEELNYVGSEPIEA
                     PDLKAAISNSMGFGGHNVVIAFTSVE"
     gene            complement(2008..2253)
                     /gene="acpP"
                     /locus_tag="VN178_07045"
     CDS             complement(2008..2253)
                     /gene="acpP"
                     /locus_tag="VN178_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWS80767.1"
                     /translation="MDREEILGKIQEITADRLGVDEGDVTSDASFREDLEADSLDLVE
                     LIMELEEQFGMEIPDEEAEKITTVEEAVDYVMEHQAA"
     gene            complement(2334..3080)
                     /gene="fabG"
                     /locus_tag="VN178_07050"
     CDS             complement(2334..3080)
                     /gene="fabG"
                     /locus_tag="VN178_07050"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWS80768.1"
                     /translation="MIEESRVAVVTGGGRGIGRAVAVRLAREGANVAISYRSNDVAAE
                     EVAEEVRAAGAKCEIFKGDVASPEDVDALFKGVGEAFGPVEILVNNAGLTRDNLMMRM
                     KESEFDEVLTTNLKGTYLCTRAALRPMIRARWGRIVNVSSVVGLVGNAGQANYAASKA
                     GIIGFTKSVAREVAQRGITANVVAPGYVETELTRSLSEEIKDQIRDQVPAGRFAEAEE
                     VAEVVAFLAGEGAGYITGQTVAVDGGMTMQ"
     gene            complement(3077..3526)
                     /gene="fabZ"
                     /locus_tag="VN178_07055"
     CDS             complement(3077..3526)
                     /gene="fabZ"
                     /locus_tag="VN178_07055"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWS80769.1"
                     /translation="MKAPLGPAQIRDLIPHRYPFLLVDRIEELEAGVRAVGVKNVTQN
                     EPFFQGHFPDYPVMPGVLIIEAMAQVGAVGVMAGGEHADKLALFAGIDGVRFRRQVVP
                     GDVLRMEVEIVRLKGRVGRGKGRATVGGERVCEADLMFAFAERGEVP"
     gene            complement(3523..>4374)
                     /locus_tag="VN178_07060"
     CDS             complement(3523..>4374)
                     /locus_tag="VN178_07060"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWS80770.1"
                     /translation="LRTCTGSSLRGEGIERAVVFPGQGTVGGETTGPARDAALAYAGD
                     EALPYQLSVFAASVDLHYRLREAGIVAEVVAGHSLGEYAAAHAAGSLGLVDGVRLVAE
                     RDRLMGEAAKKNPGGMVALIGADPDSVARAAEVADGTVVAANFNTPRQTVVSGHVDAL
                     GEVAERVRGRKVKLEVSGAFHSPLMKEAAREMDALLREVELEEPETPMVGATDGAVLT
                     TAEEVRGALRNQMLSPVRWVAVVERLLDLGVREIFEAGEDGTLTRMLRDFKRKDVVGR
                     AAGEVLS"
BASE COUNT          695 a         1586 c         1241 g          852 t
ORIGIN      
        1 caccgaccct gccacgttgc ccaacctcac cacctggtat ctgctaacca acttgccggt
       61 cccgggctct tcaaaggccg ctgaggctaa gcgcttacgt gtacctcgac ggtggagcgt
      121 gggacggggc ggatgcccag aatctccagt gcggcccggg ccgcggtctg ctcgctgtct
      181 tttatggagc tgccttcgcc ggtggcgact tcctttccgt ccacggagac cctggaggtg
      241 aagctgggcc ggtgcgccgg tccttgcttg gagatgaccc ggtacgtcgg ccttagtccg
      301 tctgcctgca aagtctcctg caggagcgtc ttccagtcac gcagctcgtc tgtatcgacc
      361 tcggcggggt tgaagatctc atcgagcgtc ctgagcacca ggtccctgtc gatgaggtag
      421 gcccccccga taatggcctc gacggtgtcg gcgatcaccg agtcgtttat cacggagctc
      481 tcgatgatct cctctatgcc ctcgctccgg cctaccatcg ccagaaacga cttcctcacc
      541 agatgggccc tgatgcgcgt gagatctccc tccagcagct cggggtagcc gtggaagaca
      601 agctccgcga cgcggctgtt caagacggag tcccccagaa actccagcct cccgttcgac
      661 tcgtaagggt ccgcgacgct cggatgggta agcgagcgct gcctcaaggg ttctggaagg
      721 atcgctatga ggtctcttat agacatctcg ttctctattc tacactcgta aacgcgatca
      781 cgacattatg cccgccgaat cccatcgaat tggagatggc ggctttgagg tcgggggcct
      841 cgataggctc gcttcccacg taattgagct cttcgcagtc ctctgccagg ttctcgaggt
      901 tgatcgtcgg tgggacgact ttgtgtttta tggccagggc gcacatgatg ccttcggtgg
      961 cgccgacggc cccgaaggag tgccccgtca tcgacttcgt gccggagacc agggcgtgcg
     1021 gcacttgcct tgcgatcgcc ttcgtctcgg gcccgtctcc ggtcggggtg gctgtggcgt
     1081 gggcgttgac gtgtcccact tcctccgcgt cgagtccgga atcttcgaaa gcgtggacca
     1141 tcgcccgctc gacgccgcgg ccgtcttcgt ctggcgccgt tacgtggtag gcgtcggtcg
     1201 tgcgtccgta tccggcgacg gcggcaaccg gttctgcgcc ccggcgctcc gcgtgctcct
     1261 cgctctccag gatcagtgca cccgcgccct cgctcatcac gaagccgtcg cggccctcgt
     1321 cgaaaggacg actggcccgg gcgggttcct cgttgcggcg gctcacggcc cgtatcgcgc
     1381 agaagccggc catggagagg ggcgttatgg gggcttccga ggaaccggct ataaccacgt
     1441 cggcgtcccc gcgccggatc agctccagac cctccgcgat cgcctcccct cccgtggcgc
     1501 aggcgaggac ggggcactgg ctaggccccg tggcgccgag cgcgatggcg agttggcccg
     1561 cggccatatt cggaaccatc atcgtgatca ggaacgggct caccctgccg ggaccacgct
     1621 cgttcatgac cctgtattcg cgctcccacg tggagagtcc cccgatgccg ctgcccatca
     1681 cgatcgccac gcgctctggc cgctcgcgga tcaggtcgac tatccccgcg ctctcggcgg
     1741 cctgaatgcc cgcggctatg ccaagctggg cgaagcggtc catcctgcgg gcctgcttct
     1801 tctttatgaa atccgtcgga tcgaaatccc aacactcacc ggcgatccgc gaaggataag
     1861 gagctgggtc gaagagcgtg atctccccta tcccgctctt gccgtgcagg gccgcgtccc
     1921 agaagttctc gacacccgtt cccaggggtg tcacggctcc tgttcccgtt acgagggcac
     1981 gacccctccc gcctccgtca gggtcggtca cgcggcctga tgctccatta cgtagtcgac
     2041 ggcctcttcg accgtagtga tcttctcggc ctcctcatcg gggatctcca tcccgaactg
     2101 ctcttcgagc tccataatga gctccaccag atccagggag tcggcctcca ggtcctccct
     2161 gaaggaagcg tcggacgtga cgtcgccttc gtcgactccc agcctgtcgg cggtgatctc
     2221 ctgtatcttg ccgagtatct cttcgcgatc caaggtttct ctcctctcca aaattcttac
     2281 aagttaccaa ctgtgggtcc aaacaatagc gcgtagtgta aacccaaaac gccctactgc
     2341 atagtcatgc cgccgtcgac ggccacggtc tgacccgtaa tgtatccggc tccttcaccg
     2401 gccaggaaag cgacgacctc ggccacctcc tccgcctccg cgaacctgcc cgccggtacc
     2461 tggtccctga tctggtcctt gatctcctcg gagaggctac gcgtaagttc ggtctccacg
     2521 tacccgggcg ccacgacgtt cgccgtgatg ccgcgctggg ccacttcccg cgccacgctc
     2581 ttcgtgaagc cgatgatccc ggctttggag gcagcatagt tggcctgccc ggcgttcccg
     2641 acgagcccaa cgactgagga cacgttgact attcgtcccc acctcgccct tatcatcggc
     2701 ctcagagccg cccgcgtgca caggtaggtg cccttcaggt tagtagtcag cacctcgtcg
     2761 aactcgctct ccttcatccg catcatcagg ttgtccctgg tgaggcccgc gttgttgacg
     2821 agtatctcga ccggcccaaa tgcttcgccc acgcctttga acagcgcatc gacatcctcg
     2881 ggcgaggcta cgtcaccctt gaagatctcg cactttgccc ccgccgccct tacttcctct
     2941 gccacctcct ccgcggccac gtcattcgaa cgatatgaga tcgccacgtt cgcgccttct
     3001 ctcgcgagcc gcaccgccac ggcacggcct atcccccttc caccgcccgt gacgacggcg
     3061 acgcggctct cctcgatcac gggacctccc ctctctctgc gaaggcgaac atgaggtcgg
     3121 cttcgcagac ccgctcgccc ccgaccgtgg cccggccctt gcccctcccg accctgcctt
     3181 tcagacggac gatctcgacc tccatcctga gcacgtcgcc gggcacgacc tgtctgcgga
     3241 acctgacgcc gtctatcccc gcaaacagcg cgagcttgtc ggcgtgctcc ccgccggcca
     3301 tgacgccgac ggcgcctacc tgggccatcg cctcgataat aaggacaccg ggcatgactg
     3361 ggtaatcggg gaagtggccc tggaagaagg gttcgttctg ggtgacgttc ttcacgccga
     3421 cggcccgcac gccggcttcg agctcctcga tcctgtctac gagcaagaag ggatagcgat
     3481 gcggtatgag gtcccggatc tgcgccggcc cgagcggcgc cttcacgaga gcacctctcc
     3541 ggcggcccgt cccactacgt cctttctctt gaagtcccgc agcatcctcg tcaaggtccc
     3601 gtcctcccct gcctcgaaga tctcacgcac cccgagatcc agcaaccgct ccaccacggc
     3661 cacccaacgc accggggaga gcatctggtt ccgcaacgcc ccgcgcacct cctccgccgt
     3721 cgtcaacacc gccccatccg tcgccccgac catcggtgtc tccggttcct ccaactctac
     3781 ttctcggagg agcgcgtcca tctctcgcgc agcctccttc atgagcggcg aatggaaagc
     3841 ccccgacacc tccagcttta ccttccgccc acgtacacgc tcggcgactt ccccgagcgc
     3901 atccacatgc ccggagacga ccgtctggcg cggcgtgttg aagttcgccg caacgaccgt
     3961 accgtcggcc acttcggcgg ccctcgcgac actatcgggg tcggcgccga tcagggcgac
     4021 cattccccca gggtttttct ttgcggcctc gcccatcagg cggtccctct cggccaccag
     4081 ccgcacacca tcgacgagac ccagactccc cgcagcgtgg gccgccgcgt actcgccgag
     4141 cgagtggccg gccaccacct ctgccacgat cccggcctcc ctcaaacggt agtggaggtc
     4201 cacggatgcg gcgaatacgg agagctggta gggcagggct tcgtctccgg cgtaggccag
     4261 ggcggcgtcg cgggcgggac ctgtggtctc tcctccaacg gtaccctgcc cggggaagac
     4321 aacggcccgc tctatccctt cgccacgcaa actagatcct gtacaggttc gcag
//
LOCUS       DATMVF010000138        10785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_204627, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000138 DATMVF010000000
VERSION     DATMVF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204627"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1602
                     /gene="hydA"
                     /locus_tag="VN178_07065"
     CDS             187..1602
                     /gene="hydA"
                     /locus_tag="VN178_07065"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWS80771.1"
                     /translation="MSVLIKGGRIVTVADDYVGDIFIEDERVSFIGESLDMQAEKVID
                     ASGKYVIPGAIDPHTHMEMPFGGTVSCDDFTSGTISAAFGGTTSHVDFCLQEPGQSLP
                     GALATWHEKIERAKPVIDVGFHLAVTDLEDGGTLEDLRKAPDEGVTSYKLFMAYKGAI
                     MVDDETLFKTMQVASETGALVMVHAENGDAIDVLVKQALAEGKTEPKWHAATRPPITE
                     GEATNRAIQLARIAGSPLYVVHVSCKEAIDPIAQARTNGWHAWGETCTQYLFIDETAL
                     DQPDFEGAKYVYTPPPRPKENQEHLWHALATDVLSVVSTDHCPFNWDGQKTLGRNDFS
                     KIPNGGPGIENRLHMLHHFGVRQGRLSMNRFVELVSTNPAKFFGMYPRKGTIAVGSDA
                     DIVVFDPEKRVKISAETHHSNIDYNLFEGTEVSGAPEVVLVRGQVIVEGDRLVGKPGA
                     GKFVRRARFGEELPGGPGRTG"
     gene            1607..2260
                     /locus_tag="VN178_07070"
     CDS             1607..2260
                     /locus_tag="VN178_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWS80772.1"
                     /translation="MSSEQTREVYERARLGQSVTLGARPAVLVVDFSRGFTDPECAMG
                     ADLTKEVEAARGLLDVAREAELPVIFTTIGFEENLKDGSLWLEKAPGLAELQVGGKWV
                     EIDPRLGRREEETVILKKGASAFFGTNLPSILVSQHVDTIIMCGATTSGCIRATAIDL
                     LQYGYPTLVPRECVGDRAQEPHEANLFDIQAKYADVVSAEEASAYIESVARKSGASV"
     gene            2295..2747
                     /locus_tag="VN178_07075"
     CDS             2295..2747
                     /locus_tag="VN178_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWS80773.1"
                     /translation="MDKRTPFQEMMEDLLGSTDASRTTLRLDLPDQDDGFDVVAAEAL
                     APGVRSISDDTSIRNLRSTSTVRFLEEQRRPLVQNDCLADDLAPPPELIELYGVRAQM
                     LAPIVREGRLVGVISVHYVPGPRQWSAEDIAALREAAERVQRELDAGV"
     gene            2764..3780
                     /locus_tag="VN178_07080"
     CDS             2764..3780
                     /locus_tag="VN178_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80774.1"
                     /translation="MMESWKNRKTLILSRTDMMGLLTPDEYNTCVEHAYRMHGEGRYY
                     MEPKGHIVLDKYPGEWEVMPSYIEEPEAAACKWVSIREENRARFDLPTVFSILIYTHP
                     ETGFPLAICDGSYHTVMRTGSAAAVSAKHLAREDSKVLAIVGAGNMAEGTLATCNEIF
                     RWEDVRVWSRSRESLDEFVRNQQPKYERFEIRASTDLEEVVRGADVVVTVTPARGPIV
                     ENGWISEGTHIAAVGADKEGDQELDPEILERARIFVDDIRQCRTDGEINVPLREGVIS
                     EDDIVGEIGQVVAGKLEGRTSETDITVFDSTGFALQDSATVPLEYERALAAGVGVEKK
                     MIST"
     gene            complement(3781..4551)
                     /locus_tag="VN178_07085"
     CDS             complement(3781..4551)
                     /locus_tag="VN178_07085"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS80775.1"
                     /translation="MSAPENPEKPTALITGAGIGIGAATARAFAAAGYRVIVTDILEE
                     EGRSVADDIGSGEVEYQRLDVMDTEETNSVVSAVQDRYGPIDTVVANAGIAHRVPLGE
                     LSDEKWDHTHEIDLKGVMRVCRAAAPAMQETGRGNMIALSSIMGVAYGWDQHVQYSAA
                     KAGVVGLVRGLAVELARDGIRVNGIAPGYIRTAQALSEEHSLGPEGLERAAEFIPMGR
                     VGEPEDIADVSVFLASEGARYITGQVLVVDGGLLVGRY"
     gene            complement(4548..5294)
                     /locus_tag="VN178_07090"
     CDS             complement(4548..5294)
                     /locus_tag="VN178_07090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HWS80776.1"
                     /translation="MTPERSRVALVTGAGSGIGRAIAERLAKNGEQVVVNDLKGEAAE
                     EVVARIKGSGGEAATAPGDVSDPEAVEQIMAAVREAYGPPEILVNNAGFLQQKRFVDL
                     TIEDFDRMIAVHLRGTFLCTRAALPEMLSQGRGIVVNVASQLGQIGGIELCHYSAAKA
                     GIIGLTKSLAREVSAQGVRVNAVAPGPINTELVLGLSEEWRDVKASELPLGRFGEPAE
                     VAETVAFLASEGAKLYVGQTLGPNSGDVML"
     gene            complement(5401..6774)
                     /locus_tag="VN178_07095"
     CDS             complement(5401..6774)
                     /locus_tag="VN178_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80777.1"
                     /translation="MAERPDQGGEGSAGQSIEGIRTTFTTRELIYPSIVAFLAWVVCV
                     YDYIMFGTLLPKMADDFGWATATSTAIATIVSVGTFFVAILVGPLIDYVGRKRSLIIT
                     TAGTGLSSLLTGLTPAALAAPFLSIVRAISGLGYSEQAVNTTYLNELYGNRKSRGFVY
                     AFVQGGWPIGVLLASAFTALLLGTVGWRGVFMLATIPALIVAALGFGLRESPRFLAMQ
                     RVRQLEKESQHEEAVEFGRHFDIDVHHVEETTLQQIFEPDIRWHTIFLALAFLTNWIG
                     IQVFAVLGTTVLTEGKGVSFGSALTVLIVSNAAAYIGYLCHGFVGDRIGRRLTIAGGW
                     VISGLAYTLMLFGPSSQGFVLTMYTIGLFFIIGPYAALLFFMGESFPTRVRGTGASFV
                     NAMGPIGAISGGALLTAFLTLTGDNMVLAAFLSGALATILSGLLMLGTRDVKDPSEAE
                     ATEGSTA"
     gene            7273..8055
                     /locus_tag="VN178_07100"
     CDS             7273..8055
                     /locus_tag="VN178_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWS80778.1"
                     /translation="MGEPRKTGKTYERIIKHIRGEISSRKLRPGDRLPPEADLARYLG
                     VSRPTVREALKVLESQNVLRSSTGPTGGTFVEAIDGTGVAVYLTDSISLLLDVDELTL
                     EELWEVREVTDVPAAELAAARRTEQDMFVIERTVEMDELKEGVAIVSDISFHRAVAEA
                     SKNRMLSLFAGSIHMTLRTLAERYVIPEDVLTEVKRISRQQHRLICEAISEQDAALAA
                     SRMREHLQLSFAVYEQAMPKKGSSDGIGEQLPAGDARRHRPT"
     gene            8209..8592
                     /locus_tag="VN178_07105"
     CDS             8209..8592
                     /locus_tag="VN178_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80779.1"
                     /translation="MEELATPVLAYVLWPLLTLAIVALWAAAALDILRHKRRGTFTNR
                     GLLGMATVVSIVAVIAVCGVCAVFAGQWSEAVSRASTDGTRASKPAGEGPRRAYIFSF
                     GLENAPYGPPLDPAGENRSEGRLSR"
     gene            complement(8707..8967)
                     /locus_tag="VN178_07110"
     CDS             complement(8707..8967)
                     /locus_tag="VN178_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWS80780.1"
                     /translation="MEWESVGTGAWAAVAFSAVFATAFSFSAWQTGVSRIGANRVLVY
                     QYLITVTGVASGIVFFGESLGIEKIIGGAVILLGVYLARRSG"
     gene            complement(9060..9590)
                     /locus_tag="VN178_07115"
                     /pseudo
     CDS             complement(9060..9590)
                     /locus_tag="VN178_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(10575..>10785)
                     /locus_tag="VN178_07120"
     CDS             complement(10575..>10785)
                     /locus_tag="VN178_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80781.1"
                     /translation="RVPLEIGGVGVMPGDIVFGDLDGVCLIPRAAEEEVFRRALEKVQ
                     KEDLVERAIEDGMSAQEAFKKFGVM"
BASE COUNT         2209 a         3344 c         3348 g         1884 t
ORIGIN      
        1 gtcttcgtcc cgtgcaagga tggcattagc cactcgccag aggaggacgc ggagccggcg
       61 gacgcggctc tcggggcaga gatcatgctc aacgccataa ggacgtttac cgcggacggt
      121 ccctgaggca tatcttcgtc agggagagaa ctcggggttg tttgtagaaa ggagtgcaag
      181 gagcatatgt ccgtactgat caagggcggc cgtatcgtta ccgtcgccga cgactacgtg
      241 ggggacatat tcattgagga cgagagggtc tccttcatcg gggagtcgct cgacatgcag
      301 gccgagaagg tgatcgacgc gagcggcaag tacgtcatcc cgggcgcgat cgacccgcac
      361 acccacatgg agatgccgtt cggcgggacg gtctcctgcg acgacttcac ctcggggacg
      421 atctctgcgg ccttcggcgg cacaacgagc cacgtcgact tctgtttgca ggagccgggg
      481 cagagcctcc cgggggcgct cgcgacgtgg cacgagaaga tcgaacgcgc aaagccggtg
      541 atcgacgtcg gcttccacct cgcggtcacc gacctcgaag atggcgggac gctcgaagac
      601 ctcaggaagg cgccggacga gggggtgacg agctacaagc tcttcatggc ctacaagggt
      661 gcgatcatgg tcgacgacga gacgcttttc aagacgatgc aggtcgcctc cgagacggga
      721 gccctcgtga tggtgcacgc cgagaacggc gacgccatag acgtcctcgt caagcaggca
      781 ctcgcggagg ggaagaccga gccgaagtgg cacgccgcga cgcgtcctcc cattacggag
      841 ggcgaagcca cgaaccgggc tatacaactc gcgcggatcg ccggctcccc actctacgtc
      901 gtccacgtct cctgcaagga ggcgatcgac ccgatcgcgc aggcgcgcac gaacggctgg
      961 cacgcctggg gcgagacctg cactcagtac ctcttcatcg acgagacggc actagatcag
     1021 ccggactttg aaggagccaa gtacgtctac acgccgccgc cgcggccgaa ggagaaccag
     1081 gagcacctct ggcacgcgct tgccaccgac gtcctctcgg tagtctccac cgaccattgc
     1141 ccgttcaact gggacggcca gaagacgctc ggcagaaacg acttctccaa gataccgaac
     1201 ggcggaccgg ggatcgagaa ccgcctgcac atgctccacc actttggcgt gcggcaaggc
     1261 cgcctctcga tgaaccgctt cgtcgagctc gtctctacca acccggcgaa gttcttcggt
     1321 atgtaccccc gcaaaggcac catcgcggtc gggagcgacg ccgacatcgt cgtcttcgac
     1381 cccgagaagc gcgtgaagat ctccgccgag acgcaccact cgaacataga ctacaacctc
     1441 ttcgagggga cggaggtgag cggcgccccg gaggtggtgc tcgttcgggg acaggtcata
     1501 gtcgaaggcg acaggctagt cgggaagccc ggcgccggca agttcgtgag gcgcgcccgt
     1561 ttcggtgaag agcttcccgg cggtcccgga aggacggggt gatcacatga gcagcgaaca
     1621 gacgcgcgag gtctacgagc gcgcccggct cgggcagagc gtgacgctcg gcgcgcgccc
     1681 ggctgtgctc gtggtcgact tcagccgtgg cttcaccgat cccgagtgcg ccatgggagc
     1741 ggacctcaca aaagaggtcg aagcggcgag gggcctcctc gacgtggcgc gcgaggctga
     1801 acttccggtc atcttcacca ccatcggctt cgaggagaac ctcaaagacg gctccctctg
     1861 gctcgagaag gctcccggcc ttgccgaact ccaggtcggg ggcaaatggg tcgagataga
     1921 cccgcgtctc ggacgcaggg aagaagagac agtaatcctc aagaagggcg cctcggcctt
     1981 cttcggcacg aacctaccct cgatcctcgt ctctcagcac gttgacacaa tcatcatgtg
     2041 cggcgcgacg acgagcggct gcatccgggc gacggcgata gacctcctcc agtacggcta
     2101 tccgacgctc gtcccgcgcg agtgcgtcgg cgaccgggcg caggagccgc acgaggccaa
     2161 cctgttcgac atccaggcca agtacgccga tgtcgtctcc gctgaagaag cctcggccta
     2221 catcgagagc gtggcccgga agtccggcgc gtcggtgtag tgaccccggc ccttacatag
     2281 agacggaacg cgttgtggac aagcggacgc cgtttcagga gatgatggag gatctgctcg
     2341 ggtcgacgga cgcgagccgg acgacgctgc gcctggacct gccggatcag gacgacgggt
     2401 tcgacgtggt cgcagcggaa gcccttgcgc ctggagttcg gtcgattagt gacgatacct
     2461 cgatacgcaa cctgcgcagt acctcgacgg tcaggttctt ggaggagcag agaaggccgc
     2521 tcgtgcagaa cgactgcctg gcggacgatc tagccccgcc gccagagctg atagagctct
     2581 acggcgtgag ggcgcaaatg ctcgcgccaa tcgtgcgcga gggtcgtctg gtaggtgtaa
     2641 tctcggtcca ctacgtcccg ggaccccgcc agtggagcgc cgaagacatc gccgccttgc
     2701 gggaggcggc cgagcgcgtg caacgggaac tcgatgcagg cgtataagtc gatagagagg
     2761 tagatgatgg agagctggaa gaaccgaaag accttgatcc tgagccgcac tgacatgatg
     2821 ggactactga cgcccgacga gtacaacacc tgcgtcgagc acgcctacag gatgcacggc
     2881 gagggccgct actacatgga gccgaagggc cacatcgtgc tcgacaagta cccgggtgaa
     2941 tgggaagtga tgccctccta tatcgaggag ccggaggcgg cggcctgcaa gtgggtctct
     3001 atccgcgagg agaaccgcgc caggttcgac ctcccgactg tcttctccat cctgatctac
     3061 acccaccccg agaccggttt cccgctggcg atctgcgatg gcagctacca caccgtgatg
     3121 cgcaccggtt ccgcggcggc ggtttccgcg aagcacctgg cacgcgaaga cagcaaggtg
     3181 ctcgccatcg tcggcgccgg gaacatggcg gaagggactc tagcaacctg caatgaaatc
     3241 ttccggtggg aggatgtgcg cgtctggagc cgctcgcgag aaagcctcga cgagttcgtt
     3301 cgcaaccagc agccgaaata cgagcggttc gaaatccggg cttcgacaga ccttgaagag
     3361 gtcgtgcgcg gggcggacgt cgtcgtcacc gttaccccgg caaggggtcc gatcgtcgag
     3421 aacggttgga tctcggaggg cacccacatc gccgccgtgg gggcggacaa ggagggtgat
     3481 caggagttgg accccgagat actcgagcgg gcacgcatct tcgtcgacga catccgccag
     3541 tgccggacgg acggggagat caatgtccca cttcgggaag gcgtgatctc cgaagacgac
     3601 atcgtgggcg agatcggtca ggtcgtcgct gggaagctcg aaggacgcac ctcggagacc
     3661 gacatcaccg tcttcgactc gacgggattc gcgctgcagg actcggcgac cgtaccgctg
     3721 gagtacgagc gggcactcgc cgcgggagtc ggcgtcgaga agaagatgat ctcgacttga
     3781 ttagtagcgg ccaacgagca acccgccgtc caccacgagt acctggccgg taatgtagcg
     3841 ggccccctca gaagccagaa atacgctcac atcggcgata tcctcgggct cccccacccg
     3901 gcccatcgga atgaactctg cggctctctc gagtccctcg ggtcctagcg agtgctcctc
     3961 ggagagcgct tgagctgtcc ttatgtagcc tggtgcgatg ccgttgacac gtatcccatc
     4021 ccgggccaac tccacggcca ggccgcgcac gaggcccacc acgccggctt tggccgccga
     4081 gtactggacg tgctggtccc atccgtaggc gacgcccatg atggaggaca gggctatcat
     4141 gtttccgcgt cccgtctctt gcatggcggg ggccgcggcc cggcacaccc tcatcacccc
     4201 tttgaggtct atctcgtgcg tatggtccca cttctcgtcg cttagctccc cgagcggcac
     4261 ccgatgagcg atgcctgcgt tggccaccac agtgtcgatc ggcccgtacc gatcctggac
     4321 cgcagacact acagaattgg tctcctcagt gtccatgacg tccagccgct ggtactcgac
     4381 ctcgcccgat ccgatgtcgt cggcgaccga cctaccttcc tcctccagta tgtccgtcac
     4441 gatgacgcgg tagcctgccg ctgcgaacgc gcgggcagtg gccgctccga tgcctatacc
     4501 ggcgccggtg atcagggccg tgggcttctc cgggttctct ggcgcactca cagcatcacg
     4561 tcacccgagt tggggccgag cgtctgcccg acgtagagct tggccccttc ggaggcgagg
     4621 aaggcgaccg tctcggccac ctccgcaggc tccccgaagc gtccgagcgg tagctcggag
     4681 gccttgacgt cgcgccactc ttcggagagg ccgaggacga gctcggtgtt gatggggcct
     4741 ggcgcgaccg cgttcaccct cacgccctgc gccgacacct cccgggccaa cgacttcgta
     4801 aggccgatga tccccgcctt ggcggcgctg tagtggcaca actcgatgcc gccgatctga
     4861 cccagctgcg acgcaacgtt gactacgatg ccgcggccct gcgagagcat ttcgggcaac
     4921 gcggcgcggg tgcacaggaa ggttccgcgc aggtgtacgg cgatcatgcg gtcgaagtcc
     4981 tcaatcgtca ggtcgacgaa ccgcttctgc tgcagaaatc cggcgttgtt caccaggatc
     5041 tccggaggac cgtaagcctc tcgaacggct gccataatct gttctactgc ttctggatcg
     5101 gaaacgtcgc cgggagcggt ggccgcttcc ccgcccgacc cctttatccg ggcgacgacc
     5161 tcctcggcgg cttcgccttt caggtcgttg acgacgacct gctcgccgtt tttggcgagc
     5221 ctctcggcga tggcgcgtcc gatcccgctt cccgccccgg tgaccagagc tacccgggag
     5281 cgttcggggg tcatcggcgt cctcctttcc tgtcccacca ctacggcggg gcaagccgta
     5341 tccggctcgc cccggccccg atacttctgt ccgacgttcg tggcatcgct caacgcgtgg
     5401 ctaggcggtg gacccttccg ttgcctcggc ctcgctcgga tcttttacgt cgcgggtgcc
     5461 cagcatgagt aatccggaga gaatcgtcgc gagcgcgccg ctgagaaacg cggcgagcac
     5521 catattgtcg ccggtcaaag taaggaacgc ggtgagcaaa gcccctccgg aaatcgcccc
     5581 gatcggaccc atagcgttga cgaaagacgc gcccgtgccg cgcactcgcg tcgggaacga
     5641 ctcacccatg aagaacagta gggccgcgta gggtccgatt atgaagaaga gcccgatcgt
     5701 gtacatggtc agcacgaagc cttgcgaact cggcccgaag agcatgagcg tgtaggcgag
     5761 accagatatc acccagcctc cggcgatggt cagacgccgg ccgatgcggt ctcctacgaa
     5821 tccgtggcag aggtagccga tgtaagcggc cgcgttggag actatgagaa cggtcagcgc
     5881 gctaccgaac gagaccccct tgccctcggt gagcacggtg gtcccgagga cggcgaagac
     5941 ctggatgccg atccagttcg tcaggaaggc cagggcgaga aagatcgtgt gccacctgat
     6001 atcgggctcg aagatctgct gcagggtggt ctcttccacg tgatgcacat ctatgtcgaa
     6061 atgccgaccg aactcaacgg cctcttcgtg ctggctctcc ttttcgagct gtctgacccg
     6121 ctgcatggcc aggaacctcg gcgactctct gagcccgaag ccgagagcgg cgactatgag
     6181 cgccggtatg gtggcgagca tgaacacgcc tcgccatccg acggtcccca gcaacagcgc
     6241 cgtaaaggcc gaagcgagca gcacgcctat cggccaccca ccttgcacga aggcatacac
     6301 aaacccccgg cttttccggt taccgtaaag ctcgttgagg taggtggtat ttacggcctg
     6361 ctcagagtag ccgagaccgg agatggcccg aacgatcgac aggaaaggcg cagctagcgc
     6421 cgcgggggtc aaccccgtca ggagggagct taggcccgta ccggcggtgg tgatgatcag
     6481 cgacctcttg cggcccacgt agtcgatcaa tggccccacc aagatggcca cgaagaaggt
     6541 tccgaccgac actatggtcg cgattgcggt actcgtcgcg gtggcccacc cgaagtcatc
     6601 cgccatcttg gggagtagcg tcccgaacat tatgtagtcg tagacgcaga caacccaggc
     6661 gaggaaagcc acgattgaag ggtagatcag ctcacgcgta gtgaaggtag tccgtatacc
     6721 ctctatcgac tgcccggcag agccctcgcc cccttgatct ggtcgttcag ccatctgagc
     6781 caacctcctt gtccgctcaa gggtccttac gacccgatga tccaacgctc cgcgccctcg
     6841 actgcgctaa acgaagcaca cccctggagg caagatagcc aaaccaggat aatttgtcaa
     6901 acaaatgttg tcggatgctt gtcccccgta tcggaatcgg cgaccctcac gtcacgacag
     6961 gaaagatcgg gcagaagcca agaacgacgc tgctccagtg gcgggctgtg cgatctagcc
     7021 gaggtgttcg cccacgatct cgcaggccca tgcccgtcct cggcggcagg tatcgggcca
     7081 ctctatgcga gaggttcctg gcgggttcat ctgcgtttca tcgccacttg gtactctgta
     7141 ggtgcttgta gatcgggagc gatgaaaagc tccgcagacc gaaatgctgt cgcactcaca
     7201 gtgagggggt gcttatctcg gatatagtat tgcagctact ccaaagggag ttgtgatatg
     7261 acaggagatg gtttgggtga accgcggaaa accgggaaga cctacgaacg gataatcaag
     7321 cacattcggg gtgagatctc ttctaggaag ctgcgcccgg gcgatagact gccgcctgag
     7381 gcggatctgg cccgatacct cggggtcagc cggccgacgg tgcgggaggc gctcaaggta
     7441 ctggagtcgc agaacgttct gaggagctcg acgggcccta cgggcgggac gttcgtcgaa
     7501 gctatagatg ggacgggcgt agcagtgtac ctaacagact ccatatctct actgctggac
     7561 gtggacgagc ttacgctcga agagttgtgg gaggtccgtg aggttacgga cgtgccggcg
     7621 gcggagctgg ccgccgcccg acgtacggag caagatatgt tcgtaatcga aaggaccgta
     7681 gagatggacg agctcaagga gggcgtcgcc atagtctcgg atatctcctt ccacagggcc
     7741 gtcgcggaag cctccaagaa caggatgctg agcctgttcg cgggttctat ccacatgacc
     7801 ttgaggacgc tggccgaacg ctacgtcata ccagaagacg tcttgaccga ggtcaagcga
     7861 atctcccggc aacagcatcg cctcatctgc gaggccatct ccgagcagga cgcggccctg
     7921 gccgcatctc gcatgcggga gcacttacag ctgtctttcg ccgtctacga gcaggccatg
     7981 cccaagaaag gatcgtcgga cggaatcggc gagcaactac cggcaggaga tgcgcgccga
     8041 cataggccga cttgatcgtc ggctgcggta gcggcacgca agggagagga cgagtttgcg
     8101 caaagcacgg acgccatacc caacttatca gcctccgacc ccggggcccg aacgagggat
     8161 tcgtaaggct ctcggcactt actttgcgtt gacccaccca ggatgaccgt ggaagagctg
     8221 gcaaccccgg ttctcgcgta cgtcctgtgg cccttgctga ccctcgcaat agtggccctc
     8281 tgggctgcgg cggcgctgga catcctgcgc cacaagcgcc gggggacgtt caccaacagg
     8341 gggttactgg gcatggccac cgtcgtttcg atcgtcgcgg tcatcgcggt ctgtggcgtc
     8401 tgtgcggtct tcgccgggca gtggtccgaa gccgtgtcga gagcgagtac cgacgggacc
     8461 cgggccagca agccggcggg cgaaggtccg aggcgtgcct acatcttcag cttcggtttg
     8521 gaaaatgccc cttacggacc accgctcgat ccggcgggcg agaacaggag cgagggccgc
     8581 ttatctcgct aagtttatta gttccagagc cgagacggtc ggccaacggc atgcgcgttg
     8641 tgagacagcg gaagccatga aaccactgtg cgtcacgatg tctgcacccc gaccctgctc
     8701 ggagaatcaa ccgcttctcc tggcaagata cacgccaagg aggataaccg caccgccgat
     8761 gatcttctca atcccaaggc tctctccaaa gaaaactatg cccgaggcaa cccccgtgac
     8821 ggtgatcagg tactggtaga ccagaacccg attggccccg atgcgggaga cgcctgtttg
     8881 ccatgccgag aaagagaaag cagtggcgaa gaccgcggag aaggccacgg ctgcccatgc
     8941 tccggtcccg acgctttccc actccaaact ggtaaggtaa ggggaagaga gcagtaggag
     9001 gaccagcccc cgaagagcag ggagtaagtc gccaccgtca gtggcgagtg acgctccagc
     9061 gcgagcatcg acagcacggt gtagctgcca aacgccatcg ccgccaggaa cacgaagagg
     9121 tccccggcca ggcttgtccc ccgggacccg aggccctcga aaaagacgat aaaaacgccg
     9181 aggatcgaga gccccacacc tacaacgccc ctccatgcgg gcctctccag cccgagcatc
     9241 aggccgagga gcagtcccca gataggggct gtagcgaaga tgagaccggt gttggcggcg
     9301 ctggtcatgc tcacaccaaa ggtgaaggcc gtctggccga tcgctacccc gaggcacccc
     9361 aagcccgcca cagggagaag gtctctcggt ttgagcctgt cggccggctc cacgatacgg
     9421 agaaggcaat acatcaggat accgcccgcc gtgaagcgaa gtgccacgat gagcagagga
     9481 ggcataaact cggccgcgta tttcgtcgct gcgtagttgg cgccccagaa gacggcggcc
     9541 agccccaaag agatctccgt aaagatggcc aacttcttct ggttcatcat gggacggaat
     9601 tgtacgcccg gatcagaaca cacgcaccga gactggaggc acggatgacg ataaacagga
     9661 acgttccgac gcacggtatc ttagagagcc tggttggaaa aaagggttgg cgaggagacc
     9721 ggccttgttg tcacttcttg acctttgaga gacgctgatc aaaacggtaa cgctcaaatc
     9781 tgtgaaacat tcgtcaatgt gagtgctttg gaggggattc cttatcaaga caagcgttct
     9841 gcgccactgt tcgtaccttg cgaatcaccc tcatgttgaa tcaggctcgt tgcagttctt
     9901 tactgctccc gagtgtaatt agcgctatgt tgggtgctct ggtttcattt gcgggcaaag
     9961 gattggaaca aagaactaga aactgacgat ctaactgagg ttagcgaggg cccccgtgca
    10021 tgctttgtct gtctacctac gagatgccca atatccagcc ctcgaagcga acctggtctc
    10081 cgtaactaat attctcgaat cgcgagcctg gcccacctca tgagcgctgg gttccagtgg
    10141 aaggacattc cttcgaccct tggcctggtc cggcaaacag cgaaagtggg ggcacgaact
    10201 gattcgagcg gagatcccgc agagtcggtc gagcctctac tcttgtaggt tcagcgagga
    10261 caccccgcag ctcaacgaga taaggctcga agatagagca gctcctctcc ccactgatat
    10321 ttgaccactt atccgtgggt gtccatgggg taccatgcgg attcaccgag ctacgaaaga
    10381 ggggaaatcg gctttctacg gaggtgtatg ggggtacctg aggatttaga gtcacctacg
    10441 taagttgact acgaatcaga agggtcgcag gttcgagtcc tcccgagcgt gctcccgaaa
    10501 gtcctacaaa tgcaggtatt tctacgcggt cccgctgcga tcttacctat ttgcggcacc
    10561 gcttcagttc cggactacat cacgccgaac ttcttgaacg cctcttgcgc actcatgccg
    10621 tcctcgatgg cccgctccac caagtcctcc ttttgcacct tctccagagc cctgcggaat
    10681 acctcctctt cagcagcgcg cgggatcagg cataccccat caaggtcgcc aaagacaatg
    10741 tctcccggca tcacccctac cccaccgatc tccagcggga ctcgg
//
LOCUS       DATMVF010000139         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_390188, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000139 DATMVF010000000
VERSION     DATMVF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390188"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VN178_07125"
     CDS             complement(<1..695)
                     /locus_tag="VN178_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS80782.1"
                     /translation="MLEDKVALITGASQGLGRALALAFAREGARVAINARSERSIGPV
                     AEEAEDTGAEVLALAADVSKGADVDRMVGETVERFGKIDVIVNNAGLLGPRVGIEEYP
                     EDEWREVIDANLTGPFLVSKAAIPHLPEGGSVINVVSGVSVEGRAEWGAYSVSKFGIE
                     GLTQILAAELAGRGIRVNAVDPGGMRTQMRAAAYPEEDPTTRITPEENTAVFLYLASD
                     ESRGVTGERFKAQ"
     gene            complement(880..1014)
                     /locus_tag="VN178_07130"
     CDS             complement(880..1014)
                     /locus_tag="VN178_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80783.1"
                     /translation="MTEERRSADGMLGKGLRHLVALGDHPESANQIQREVLAKQLLGR
                     "
     gene            complement(1028..1477)
                     /locus_tag="VN178_07135"
     CDS             complement(1028..1477)
                     /locus_tag="VN178_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWS80784.1"
                     /translation="MHRSTDAEEAVDRAIPLEVGESVSFSKTVGESDVYLFAGITGDF
                     SPNHVNKEVMAGTPYKERIAHGVLSIGFASTTSTLMIEKSGAKAVSLGYDRIRFVGPI
                     FVGDTVTVTYTISEIDEENLRTRADIEVANQRGEVCTVAQHILKFLA"
     gene            complement(1461..>2205)
                     /locus_tag="VN178_07140"
     CDS             complement(1461..>2205)
                     /locus_tag="VN178_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWS80785.1"
                     /translation="LATINGRKSDPPTLVGSAVVDQHAAVLGALGVLAALYERRDTGK
                     GKRVDSNLLNSALDLQIEPFIYHLNGQLYDRSETGVSSRFHQAPYGIFETADGYICIS
                     LTPPEKMAEAFGDDTFREWSEEDQFRRREEVNAHIAGHVRTRPAEHWFGVFDEVGIWY
                     APVNDYEDVEEDPQVVHNQAIMTFEHPRAGKVRVLSHPVRYDGEAPPLRMLPPELGEH
                     TEEVLAEVGYGQEEIEDLKSEGVVRAQEH"
BASE COUNT          342 a          740 c          655 g          468 t
ORIGIN      
        1 tcctgcgcct tgaaacgctc acccgtaacg cctctcgatt cgtctgaggc caggtacagg
       61 aagacggcgg tgttctcttc cggggttatg cgggtagtgg ggtcctcttc cgggtaggcg
      121 gcggcgcgca tctgggtgcg catcccaccc gggtcgacgg cgttcacgcg gatcccgcgc
      181 cccgctagct ctgcggccag gatctgggtg agtccctcta tcccgaactt gctcaccgag
      241 taggcgcccc actcggccct tccctcgacg ctgacaccgc tgaccacgtt gatcacggag
      301 ccgccctcgg ggaggtgtgg gatcgccgcc ttcgagacca ggaaaggacc ggtgaggttg
      361 gcgtcaatga cctctctcca ctcgtcctcg ggatactctt cgatcccgac cctgggacca
      421 agcagaccgg cgttgttgac gatcacgtct atcttcccga aacgctcgac cgtctcgccg
      481 accatcctat cgacgtcggc acccttcgac acgtccgccg cgagggccag gacttcggcg
      541 cccgtatctt cggcctcttc ggcgaccggg ccgatagacc tttcactgcg ggcgttgatc
      601 gccaccctgg cgccctctct cgcgaaggct agtgctagcg ccctgccgag accctggctc
      661 gcgccggtga tgagtgcgac tttgtcttcc aacatgttac ttcccttcgg agcatttcag
      721 cacgcgccct gactggcgct tgtcagcacg ctccagcctg tggctgtcgc ttgtcagcgc
      781 ttcaacattt cagcttagta cgaaaatcgg actcgcgctt gagatttgcc tgccccgcca
      841 ggatggttga gcgatccctg gaagcttctg gctgacgact catcgtccga gtagctgttt
      901 ggcgaggact tcgcgctgta tctggttggc gctctcggga tgatccccca gtgccacgag
      961 gtggcgcagt cccttgccga gcatgccgtc ggcggatctc ctctcctcag tcaagcggtg
     1021 agcctcccta cgccaggaac ttgaggatgt gctgggccac ggtgcacacc tcgccgcgtt
     1081 ggtttgcgac ctcgatatct gcgcgcgtcc tcaagttctc ctcgtcgatc tcggagattg
     1141 tgtaggttac cgtgaccgtg tccccgacga agatcggacc tacaaacctg atgcggtcgt
     1201 agcccaaaga gacggccttg gctcctgact tctcgatcat gagcgtcgag gtcgtcgagg
     1261 cgaatcctat cgagagcacg ccgtgggcga tgcgctcctt gtacggcgtc cctgccatga
     1321 cctctttgtt gacgtggttc gggctgaagt cgcccgtgat gccggcgaac aggtacacgt
     1381 cggactcgcc gacagtcttg gagaacgaaa ccgactcgcc gacctccagg ggtatggccc
     1441 gatccaccgc ctcttctgcg tcagtgctcc tgtgcacgca ctactccttc cgatttcagg
     1501 tcttcgatct cctcctgccc gtagcccacc tcggcgagta cctcctccgt gtgctccccg
     1561 agttcggggg gcagcatgcg cagtggtggc gcctcgccgt cgtagcggac ggggtgggag
     1621 agcacccgca ccttgccagc cctcggatgc tcgaaggtca ttatcgcctg gttgtggacg
     1681 acctgcgggt cctcctcgac gtcctcgtag tcgttgaccg gcgcgtacca gatacctacc
     1741 tcgtcgaaca ccccaaacca gtgctccgcc ggtctcgtcc tgacgtgccc ggcaatgtgg
     1801 gcattgacct cttccctcct gcggaactgg tcctcctcac tccactcgcg gaaggtgtcg
     1861 tccccgaacg cctcggccat cttctccggc ggcgtcagcg agatgcagat gtagccgtcg
     1921 gccgtctcga agataccgta gggcgcctgg tgaaaccgcg aggagacgcc cgtctcgctg
     1981 cggtcgtaca gttgtccgtt gaggtggtag atgaacggct ctatctgaag gtccagcgcc
     2041 gagttcagca aattgctgtc aacccgtttc cccttacccg tgtcccgacg ctcgtagagg
     2101 gcggcaagga cgccaagagc ccctagtacc gccgcgtgct ggtcgacgac ggcggagccc
     2161 accagggtgg gagggtcgct tttgcgcccg ttgatcgtcg ccagg
//
LOCUS       DATMVF010000140         4848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_657161, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000140 DATMVF010000000
VERSION     DATMVF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657161"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VN178_07145"
     CDS             complement(<1..383)
                     /locus_tag="VN178_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80786.1"
                     /translation="MSGLIGVEEQVDKDFHRALLKASLHRLRDKLRRDLAHERLLSFD
                     ETKGALVHWSQVYRGMRIVEVEKITGSVGRCRDFDGSFLPLRVSMAHRWGRVDRAYHK
                     GIELPAVSLYKIGEAYFVRDGNHRVS"
     gene            complement(739..1668)
                     /gene="rapZ"
                     /locus_tag="VN178_07150"
     CDS             complement(739..1668)
                     /gene="rapZ"
                     /locus_tag="VN178_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWS80787.1"
                     /translation="MYPRRGGRLDVGEQATDSLASGKRRIVIITGLSGAGKSNALQAF
                     EDAGYFCIDNLPPRMIPAVMETTAHGAGGPDGVVVVVDMRGRRYFGEELERSLEAVEQ
                     EKGWEHSILFIDSDDPTLVRRYKESRRPHPAARNGDVLEAIRRERQDLANLREMADLV
                     VDTSGYSALDTRLRFKKLAESFSSRMTVSLISFGFKHGTPLDVDTLLDVRFLPNPHYD
                     PELRPLIGHDAPVREAVLGSEDCQEFLEKTIDFFSFLIPRYAAEGKTYFTVGIGCTGG
                     RHRSVAITEELAKRLEDEDTGIDLFIRHRDVEK"
     gene            complement(1883..3721)
                     /gene="uvrC"
                     /locus_tag="VN178_07155"
     CDS             complement(1883..3721)
                     /gene="uvrC"
                     /locus_tag="VN178_07155"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWS80788.1"
                     /translation="MPENGSPDRSHLPTSPGVYIFEDADEKVIYVGKAKNIRSRVANY
                     FTRSGDGRPKIAELRERVRQIDFISTTSETEALVLEANLIKRHRPRFNASLKDDKSYP
                     YIVVTTGDEYPRVFATRSSHDPRHRYFGPFPSAGSVHATLDVLNKTFPFRKCRGPEPG
                     RRSGVPCLNYHIGRSAAPCIGAVSKEDYDGIIADVISFLEGRVDGLIREREAAMREAA
                     RDTNFERAAKLRDEISALSHVRDRQRATIASEDSFDAFGAYTEGETACVQVFAVREGQ
                     IVNRDSFLLDNYGESRAEDVSLQFVPQYYGTAAVPREVLVQSDDREEELATLAEHLSG
                     LRGTGVEVRRPRRGDKRRILEMAVRNAELGLEHERMLDEARRNRVASTLDTLREELAL
                     PKLPVRIECYDISNTMGTNSVASMVVFQGGRPAKDQYRRFKIRTVEGADDFASMAEVI
                     RRRLQRLDAGDEKFSPAPDLIVLDGGKGQLSAVIPVLEEMKVGDELPDIPLRSLAKRE
                     EEVFEPGRPEPVVLERNSPELHLLQRVRDEAHRFANAYHTKLRGRAMTASVLDDLPGV
                     GPERKKKILAHFGTPEAFLNASLEELESVPGLPGRVAREVHARVHR"
     gene            complement(3760..4494)
                     /locus_tag="VN178_07160"
     CDS             complement(3760..4494)
                     /locus_tag="VN178_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80789.1"
                     /translation="MSSRSNGSEERTTGLRPRYRRAFGCVTWVYMGMLVFLAVNFLLV
                     LPSRYVGSGELYGYLPPALAYLGTLLILPGMVLAALLGARTFRTPRKRGTRIGAGVGA
                     LIGYSGFFGLAWLAIALGLDRRDQAFRTVAFPDLGGSLAFYAFVPLTLLATGLVISAL
                     FTRGADFERRRRLVYAGAALAVLAGLGVVVTDFDPLGVLGALISTASAAIGGYVSGAG
                     YARAGGDDMIPPGAEIRPREPWRKRR"
     gene            complement(4478..>4848)
                     /locus_tag="VN178_07165"
     CDS             complement(4478..>4848)
                     /locus_tag="VN178_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80790.1"
                     /translation="GVSDPFGRSWELEGDPQALDIAVSDGFLHYGDYPDALERLWGCL
                     HSPRCGDVVLSATPGYTFGEISGGYHTASDHGSLHASDSNVFVLASGVPAPRRITDVA
                     PTLLSHFGADLVAGDRVLQE"
BASE COUNT          875 a         1466 c         1591 g          916 t
ORIGIN      
        1 gagacgcggt ggttgccatc gcgcacgaag taggcctccc caatcttgta gaggctcacc
       61 gcaggcaact caattccctt gtggtaggcg cgatctacac gtccccacct gtgggccatg
      121 ctaaccctga ggggcaggaa agacccgtcg aaatcccggc atcttccgac actccctgtt
      181 atcttctcga cctcgacgat cctcatccct cggtagacct ggctccagtg caccagggct
      241 cctttcgtct cgtcgaacga gagcaagcgc tcgtgggcta aatccctgcg cagcttgtct
      301 ctcaagcggt gcagagaagc cttcaggagg gcacgatgga agtccttgtc gacctgctct
      361 tctacgccta tcaacccgct catcggtttt cccttcgctc tttcctggta tggtcactct
      421 agcggcgcca ctatcccggg aggtagggaa tggtttcctg acttgttcgg gcaatatttc
      481 ccgggaagtt gtcccgagtt tgagctatta cgcaaaacga tcgagccttc tctgaggact
      541 atgagtgacc gttagtgtct aatgcctaat ctggtagatc gtttctggtc ggagacgcga
      601 aacacctagc gcgatggatt cccgctttcg cgggaatgac aggaataccg ggaagaacag
      661 ggtaggagtc gcaatcacca agatcataca tcgcagaagt cggattccgt agaaagcctg
      721 acacctgtaa cctgaagcct atttttctac gtctctgtgc ctgataaaga ggtcgattcc
      781 ggtgtcttcg tcctcgagac gtttggccag ttcctcggtg atggcgacgg agcggtgtct
      841 gccgccggtg cagcctatgc cgacggtgaa gtaggtcttg ccttctgcag cgtagcgggg
      901 aatgaggaag gagaagaaat cgatggtctt ctcgaggaat tcctggcagt cttctgagcc
      961 gaggaccgct tcccggacgg gcgcgtcgtg gcctattagc ggcctgagct cggggtcgta
     1021 gtgggggttg gggaggaaac ggacgtcgag gagcgtgtct acgtcgaggg gggtgccgtg
     1081 cttgaaaccg aaggagatga ggctgacggt catacggctg gagaaggatt ccgcgagttt
     1141 cttgaaccga aggcgagtgt cgagcgcgga gtagcccgag gtgtcgacga cgagatcggc
     1201 catctcccgc aggttcgcga gatcctgcct ctcgcggcgt atggcctcga ggacatcgcc
     1261 gttgcgggcc gcggggtggg gcctgcggct ctccttgtag cgcctgacca gcgtggggtc
     1321 gtcggagtcg atgaacagga tgctgtgctc ccagcccttc tcctgctcca cggcctccag
     1381 actgcgctcg agttcctcac cgaagtaccg gcgtccgcgc atgtccacca cgacgaccac
     1441 cccgtcaggt ccgccggcgc cgtgcgccgt agtctccatg acggccggga tcatacgcgg
     1501 agggaggtta tctatgcaga agtaaccggc gtcctcgaag gcctggaggg cgttgctctt
     1561 gcccgcgccc gagagccccg tgatgatgac gatcctgcgc ttgcccgagg ccagggagtc
     1621 agtagcctgc tcacccacgt cgagtcgtcc gcccctcctg ggatacacaa accatctcgg
     1681 aagtcgagat tagcattgtg ggtcttcgtc tgcaataggc ttcaggtgta aggccacggg
     1741 cttcagcagt ggattcccgc tttcgcggga atgacgcatg agatggcctg agcgtgccac
     1801 atagcccaaa cgcggttccg ttcgacagaa cgacacacag caacagacac aagaacacaa
     1861 gatcaaacct gatgcctgta acttaccgat gcactcttgc gtggacttcg cgggctactc
     1921 ttccagggag tcctgggacg gactccagtt cttcgagaga ggcgttcagg aatgcttctg
     1981 gggtgccgaa gtgggccagt atcttctttt tgcgttcggg gccgacgcca gggaggtcgt
     2041 cgaggaccga ggcggtcatg gccctgccgc ggagcttggt gtggtaggcg ttggcgaaac
     2101 ggtgggcctc gtcgcggacg cgctggagga ggtgcagttc gggggagttg cgctccagga
     2161 ccaccggctc cggacggccc ggctcgaaga cctcctcctc gcgtttggcg agcgaacgca
     2221 gggggatgtc gggtagctcg tcgccgacct tcatctcctc gagaacgggg atgacggcgg
     2281 agagctgtcc cttaccgccg tcgagtacga tcaggtcggg ggcaggcgag aatttttcat
     2341 cgccggcatc gaggcgctgc agccggcggc ggattacctc ggccatgctt gcgaagtcgt
     2401 cggcgccttc gaccgtcctt atcttgaagc gccggtactg gtccttcgcc gggcgtcctc
     2461 cctggaacac gaccatagag gccacggagt tggtccccat ggtgttcgag atgtcgtagc
     2521 actcgatccg gaccggaagc ttggggagcg cgagctcttc gcgcagagtg tcgagcgtgg
     2581 aggcgaccct gttgcgcctg gcttcatcca gcatccgctc gtgctccagc ccgagttcgg
     2641 cgttacgcac ggccatctcc agaatccggc gtttgtcccc ccgcctggga cggcgtacct
     2701 ccacgccggt ccccctcaag cccgagaggt gctcggcgag cgtagcgagt tcctcctcgc
     2761 ggtcgtctga ctggacgagg acctcgcgtg gaacggcggc cgtaccgtag tactggggga
     2821 cgaactggag cgagacgtcc tcggcccgcg actcgccgta gttgtcgagg aggaaagagt
     2881 cgcggttcac gatctggcct tcacgcacgg cgaagacctg tacgcaggcc gtctcgccct
     2941 cggtgtaggc gccgaaggcg tcgaaggagt cctcagaagc gatggtcgcc ctctggcggt
     3001 cgcgaacgtg ggagagggcg gagatctcgt cgcgcagctt cgccgcccgc tcgaagttcg
     3061 tgtccctggc ggcctcgcgc atggcggcct cgcgctcgcg gatcaaaccg tccacccgcc
     3121 cctccagaaa ggagatgacg tcagcgatta tcccatcgta gtcctccttg gagacggccc
     3181 cgatgcacgg agccgccgaa cgcccgatgt ggtagttgag acaaggcaca ccgctgcgcc
     3241 gcccgggctc gggaccgcgg cacttgcgga aggggaaagt cttgttcagt acgtcgagcg
     3301 tcgcgtggac gctgccggcg ctcgggaaag gtccgaagta gcggtggcgc ggatcgtggg
     3361 aggagcgggt ggcgaagacc ctggggtact cgtctccggt cgtcaccacg atgtaggggt
     3421 agctcttgtc gtccttgagc gaggcgttga agcgcggcct gtggcgcttt atcaggttcg
     3481 cttcgaggac gagcgcctcg gtctcgctcg tcgtggagat gaagtctatc tgcctgacgc
     3541 gctccctgag ctccgctatc ttcgggcgtc cgtcgccgga acgggtgaag tagttggcga
     3601 cccggctgcg gatgttcttc gccttgccga cgtagataac cttctcatcc gcatcctcga
     3661 agatgtatac gccgggtgag gtgggaagat gtgatctatc gggggatccg ttttcgggca
     3721 tcgagtgaga ttataccgcg aagagttaca tatcctcgct tagcgacgct tacgccaggg
     3781 ctctcggggg cgtatctcgg cccctggtgg gatcatgtcg tcaccgccgg ctctggcgta
     3841 gccggctccg ctcacatagc cgccgatggc tgctgatgcg gtggagatca gggctcccag
     3901 gacaccgagc gggtcaaaat ccgtaacgac gacgccgagc cccgcaagca ccgccagcgc
     3961 agcccctgca tagacgagcc tgcgccgccg ctcgaagtcg gcccctctgg taaagagggc
     4021 ggatatgacg aggcctgtcg caaggagcgt cagtggaacg aaggcgtaga aggcgaggct
     4081 ccctccgagg tcggggaagg ccaccgtgcg gaacgcctgg tctcgccggt ccagcccgag
     4141 ggcgatagcg agccaggcca ggccgaagaa gccgctgtag ccgatcaggg ctcccacgcc
     4201 ggctccgatg cgggtcccac gcttgcgcgg tgtccggaat gtacgggctc cgagcagcgc
     4261 tgcgagtacc attccgggta ggatcaggag ggtcccgagg tacgcgagcg cggggggaag
     4321 atatccgtag agctccccgc tacctacgta acgggacggc aacacgagga gaaagttgac
     4381 cgcgaggaag accagcatcc ccatgtacac ccaggtaaca catccgaacg cccgacggta
     4441 acgcggcctg agcccagtag tgcgctcctc ggagccgtta ctcctggagg acacggtctc
     4501 ctgcgaccag atcggcgccg aagtgagaga ggagcgtcgg ggccacgtcc gtgatcctgc
     4561 ggggcgccgg cacgccgctc gccagcacga acacgttgga gtcgctggcg tgtaaggagc
     4621 cgtggtcgct cgccgtgtga tacccgccgg agatctcacc gaacgtatac cccggtgtcg
     4681 ccgagagcac gacgtccccg cacctcggcg agtgcaggca accccacagg cgctccaggg
     4741 cgtcaggata atcaccgtag tgcaagaatc catcggagac ggcgatgtcg agggcttgcg
     4801 ggtcgccttc gagctcccag gaccgcccga acgggtcgct cactcccg
//
LOCUS       DATMVF010000141         5426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_13798, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000141 DATMVF010000000
VERSION     DATMVF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13798"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1812
                     /gene="iolD"
                     /locus_tag="VN178_07170"
     CDS             <1..1812
                     /gene="iolD"
                     /locus_tag="VN178_07170"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HWS80791.1"
                     /translation="DGIEHRFFAGCFGIFGHGNVAGVGQALQQYSDGLPYYQARNEQA
                     MVHTASAYARQNNRLKTFACTTSIGPGATNMVTGAALATINRLPVLLLPGDVFASRGP
                     DPVLQQLETPHDGTLSVNDCFRPVSRYFDRISRPEQVIGSALAAMRVLTDQAETGAVT
                     LALPQDVQTEAFDYPEAFFEKRVWHVGRPLPDAGAMDRATVMIRASRRPMIVAGGGVI
                     YSEATEALRRLCEHTGIPVGETQAGKGSMPYDHPCSLGAVGATGTFAANRAAREADLV
                     IGIGTRWSDFTTASKTAFLNPNVRFVNINMAGFDASKHAGLALVGDARSTLQALDEAL
                     TGYEVEHGYREQNAAESREWDREVGRLYDLGHGPLPAQSEAIGVVNTFSDPEDVVVCA
                     AGSMPGDLHKLWRTRDPKGYHVEYGYSCMGYEISGGLGVKMAAPEREVYVMCGDGSYL
                     MMNSEIVTSIQEGRKLTIVLVDNSGFSSIGALSRSVGSDGFGTHYRYRKGGSIGLDSE
                     EAPGDVLPVDLAKGAESLGAYVIRTENIEDLKSALAAAKEVDGTVVIHIPVDRYEGVP
                     DYDSFWDVPVSEVSEMESVTSAREEYAENKKAERRYL"
     gene            1828..2628
                     /gene="iolB"
                     /locus_tag="VN178_07175"
     CDS             1828..2628
                     /gene="iolB"
                     /locus_tag="VN178_07175"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HWS80792.1"
                     /translation="MPDLLVTSEDKPDRDGVLIKVTPESAGWEYVGFEVLRIEAGRSA
                     SRHTGGDEVCLVPLSGTCTVSAGDEQWKDIGGRENVFDGPPSAVYLPPGTEYTVRAST
                     RLEIALASAPADQGVDPMVVRPQDIEVEVRGSGNMEREIRPILMEDRGAERLLVVEVL
                     TPNGHWSSYPPHKHDRDDPPRERYLEETYYHKIRPVRGFALQRVYTEDGSLDETLTFH
                     DGDVVLVPEGYHVVSAPPGYDLYYLNVMAGPVREWKIKNHPDHEWLLG"
     gene            2644..3978
                     /locus_tag="VN178_07180"
     CDS             2644..3978
                     /locus_tag="VN178_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS80793.1"
                     /translation="MEHTTTVPRELSRRDFLKVGGAGLAGAVLLGTAGCGGGGEQSGG
                     PTELYFTAPPDTSGTTSKLINQFNEKNKGTYRVIFREGSSDTGQRLDKLRTQFQAGGE
                     EIDVILGDVIWTAELAASGWISDLSDRFPESRQQAYLPGSVEAIIFEGKPYGMPWFTD
                     TGLLYYRKDLLNQSGYDGPPKTWDELKQMTKKVSREAGIKFGFLFQGARYEGGVCDGL
                     EFIWGHDGNVLDPNDPTRVIVDSPQAIAGLATERSMITDGISPEAVTVYKEDESAGAF
                     LNGDGIFLRNWPYVYALVGDPAESKLKPEQVGVSELPSADGKPGNGTVGDQPLYISAS
                     SKNPDAAWKFIEFLTAPEQQRLRAVEGAYLPTLSDLYDDPEIQESVPVVPLAKVALQH
                     TLPRPVSPYYAEMSLEMAEQFNASLTGDVTPEEAARTMKKGLESIIQQGEEA"
     gene            4007..4984
                     /locus_tag="VN178_07185"
     CDS             4007..4984
                     /locus_tag="VN178_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS80794.1"
                     /translation="MRVGLVGAGFMGGVHLNAYAGIPEVEVVGVADARVEAAVAGAEM
                     VGARPYASYEELVAAEDVEVVDVCLPTAFHRDLALRAAGEGRHVILEKPIARTMEDAQ
                     QILGAFSGDGPRLFVGHVVRFFPEYVGIKEKIDAGDLGTVGVVRTSRRSPFLLGWNDW
                     YADWRVSGGVLLDLVIHDFDYLRWTLGEVERVYARGLLGREYNRLDYVLVTLRFESGA
                     IAHVEGHWGYPGPFNYSIEVAGSDALLTVDSTEPASLQLIGGAPDEVPDLASGKSPYE
                     KELEHFIHCIATGEEPIVQAKDAREALRIGLAATQSVLTGEPITLGGRT"
     gene            4981..>5426
                     /locus_tag="VN178_07190"
     CDS             4981..>5426
                     /locus_tag="VN178_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS80795.1"
                     /translation="MSVRIGIVSFAHVHSPAYAAVLRDLAIADFVGISDEDRDRGAEA
                     AELFGVRFFDNVASLMDEVDAVVVCSENKNHARDVIPALQGGVHVLCEKPIATTVEDA
                     RAMIRASEISGSQLRTAFPVRYLPSVVRARELVRGGALGRVLAVNGT"
BASE COUNT         1028 a         1767 c         1741 g          890 t
ORIGIN      
        1 gacgggatcg aacaccgctt cttcgccgga tgcttcggta tcttcggcca cggtaacgtc
       61 gccggtgtcg gccaggcact ccaacagtac tctgacggcc tcccttacta ccaggcccgc
      121 aacgagcagg cgatggtgca cacggcctcg gcctacgccc gccagaataa ccggctcaag
      181 accttcgctt gcaccacctc catcggaccg ggcgccacca acatggttac gggagcggcc
      241 ctcgccacga tcaaccgact gcccgtactg ctcctccccg gtgacgtctt cgcctcgcgc
      301 ggaccagacc ccgtcctgca acagctcgag accccccacg acgggaccct ctcggtcaac
      361 gactgtttcc ggccggtctc tcgatacttc gacaggatct cccgtcccga gcaggtgata
      421 gggtccgccc tcgcagcgat gcgcgtgctc acagaccagg ccgaaacggg ggccgtcacc
      481 ctcgcgctcc cacaggacgt gcagacggag gctttcgact atcccgaagc gttcttcgag
      541 aaacgagtct ggcacgtagg acgccctctg ccagatgccg gcgctatgga ccgtgctacc
      601 gtcatgatcc gcgcttcccg ccggcccatg atcgtggccg gcgggggcgt catctactcg
      661 gaggccacgg aagcgctgcg gcgcctgtgc gaacacaccg gcatccctgt cggcgagacg
      721 caggcgggga aaggctccat gccctacgat cacccttgct ctttgggcgc cgtcggcgcg
      781 actggcacct tcgccgcgaa ccgcgcggcc cgcgaggccg acctcgtcat cgggattggc
      841 acccgctggt cggacttcac cacggcctcc aagaccgcct tcttgaaccc gaacgtgcgc
      901 ttcgtcaaca taaacatggc cggcttcgac gccagcaagc acgccggact tgccctcgtc
      961 ggcgatgccc gctccaccct ccaggcgctc gacgaggctc tcaccggcta cgaagtggag
     1021 cacggctaca gggagcagaa cgccgccgaa tctcgagagt gggataggga ggttgggcgg
     1081 ctatatgacc ttggacacgg accgctaccg gcccagagcg aggccatagg ggtcgtcaac
     1141 acgttctcag atcccgagga cgtcgtcgtc tgcgcggccg gttcgatgcc gggtgacctg
     1201 cacaagctct ggaggacgcg cgacccgaaa ggctaccacg tcgaatacgg ttactcgtgc
     1261 atggggtatg agatctccgg cggcctcggc gtgaagatgg cggcccccga gcgcgaggtc
     1321 tacgtgatgt gcggcgacgg ttcctacctg atgatgaact ccgagatcgt cacctccatc
     1381 caggagggcc gcaagctcac catagtcctg gtcgacaatt ccgggttctc ctctatcggg
     1441 gcgctctccc gctccgtcgg ctctgacggc ttcggcaccc actatcgcta ccgcaaggga
     1501 gggtccatcg gcctcgactc ggaggaggcg ccaggagacg tcctgcccgt cgacctggcc
     1561 aaaggcgcag agagtctcgg tgcgtacgtg atccggacgg agaacatcga ggacctgaaa
     1621 agtgctctcg cagccgccaa agaagtcgac gggaccgtgg tcatccacat ccccgtcgac
     1681 cgctacgaag gcgtcccgga ttacgattcc ttctgggatg tgcccgtctc cgaggtctcc
     1741 gagatggagt ccgtgacctc ggcccgcgag gagtacgcgg agaacaagaa agcagaacgc
     1801 agatacctct agaaaggagc tttcggtgtg cctgacctgc tcgttaccag cgaggacaaa
     1861 cctgaccgcg acggcgtcct gatcaaggtc accccagagt ctgcgggctg ggagtacgtc
     1921 ggattcgagg tcctcaggat cgaggcgggc cgcagcgcga gccggcacac agggggcgat
     1981 gaggtgtgcc tggtaccgct ctcggggact tgcacggtgt cggcaggaga tgagcagtgg
     2041 aaagacatcg gtggcaggga gaacgtgttc gatggtcctc cttctgcggt ctatctgccg
     2101 cccggtacgg agtacacggt acgagcatcg acgaggttgg agatcgccct cgcctcggcg
     2161 cctgccgatc agggcgtcga cccgatggtg gtaagacccc aggacatcga ggtggaggtt
     2221 cgcggctcgg gcaacatgga gcgggagata cgtccgatcc tgatggagga ccgcggggcg
     2281 gaacggctac tggtggtcga ggtccttact ccgaacgggc actggtcgag ctatccgccg
     2341 cacaaacacg acagggacga ccccccgcgc gagcggtacc tggaggagac ctattaccac
     2401 aagatccgtc ccgtaagagg cttcgccttg cagagggtgt ataccgaaga cggctcgctc
     2461 gacgagaccc tgacgttcca cgacggtgat gttgtgctcg tgccagaggg ttaccacgtg
     2521 gtctcggcgc ctccgggcta cgacctctac tacctcaacg tcatggcagg gccggtacgc
     2581 gagtggaaga taaaaaacca tcccgaccac gagtggttgc tcggctagcg aagggagaag
     2641 cgtatggagc acacgacaac cgtacccagg gaactctcgc gcagggactt cctcaaggta
     2701 ggcggggcag gactcgcagg cgcggtgctc ctcgggaccg cgggttgcgg gggaggaggc
     2761 gagcagagcg ggggcccgac cgagctgtac tttactgccc cgcccgacac gtcggggacc
     2821 acctccaagc tcatcaacca gttcaacgag aagaacaagg gcacctacag agtcatattc
     2881 cgcgagggca gctcggatac gggccagcgc ctggacaagc tgaggaccca gtttcaggcc
     2941 ggcggagagg agatcgacgt gatcctcggc gacgtcatct ggaccgccga gctggccgcg
     3001 agcggttgga tctccgacct ctccgaccgc ttcccggaga gccggcagca ggcgtacctg
     3061 ccgggctccg tcgaagccat aatcttcgaa ggaaagcctt acggcatgcc gtggttcacg
     3121 gacacgggcc tcctctacta ccgcaaagat ctgctgaatc agagcggcta cgacggtcca
     3181 ccgaagacct gggacgagct caagcagatg accaagaagg tgagtagaga agcgggcatc
     3241 aagttcgggt tcctcttcca gggcgcgagg tacgagggtg gggtgtgcga cggcctcgaa
     3301 ttcatctggg gccacgatgg taacgtcctc gaccccaacg accctacaag agtcatcgtc
     3361 gatagtcccc aggccatagc gggcctggcg accgagcgga gcatgatcac cgacggcatc
     3421 tcgccggagg ccgtcacggt ctacaaggag gacgagtcgg caggggcctt cctgaacggc
     3481 gacgggatct tcctgcgcaa ctggccctac gtctacgccc tcgtcggcga cccggcggag
     3541 tcgaagctaa aacccgaaca ggtgggtgtt tcggagctgc cgtccgccga cggcaaaccc
     3601 ggcaacggca cggttggtga tcaaccgctg tacatcagcg ccagctcgaa gaacccggac
     3661 gccgcctgga agttcataga gttcctcacc gcgcccgagc agcagcggtt gagggccgtc
     3721 gaaggcgctt acctgccgac cctctcggat ctctacgacg accctgagat ccaggagagc
     3781 gtgcccgtcg ttcccctcgc aaaagtggcg ctccagcaca ccctcccgcg cccggtctcg
     3841 ccctactacg cggaaatgtc cctcgagatg gccgagcagt tcaacgcctc gctaaccggg
     3901 gacgtgacac ccgaggaggc ggcgcgtacc atgaagaagg ggctcgagag catcatccag
     3961 cagggcgagg aagcttagcc ttgaacgacc tgagaggggg gatcggatgc gggtcggtct
     4021 tgtaggcgcc ggctttatgg gcggcgttca tctgaacgcc tacgccggca tccccgaagt
     4081 ggaggtggtc ggcgtcgccg acgcccgcgt cgaggccgcc gttgcgggtg cggagatggt
     4141 cggggcgcgg ccctacgcct cctacgagga gctcgtcgcg gcggaagacg tcgaggtagt
     4201 cgacgtctgc ctgccgactg ccttccacag ggacctggca ctcagggcgg cgggcgaagg
     4261 caggcacgtc atcctcgaga agccgatagc ccgcaccatg gaggacgcgc agcagatctt
     4321 aggcgcgttc tcgggtgacg gtccccgcct cttcgtcggc cacgtcgtcc gtttcttccc
     4381 cgagtacgtg gggatcaagg agaagatcga cgccggagac ctcgggacgg tcggggtggt
     4441 ccgtaccagc cgccgctcac ccttcctcct cggctggaac gactggtacg ctgactggcg
     4501 cgtgagcggc ggtgtccttc tggacctggt gatccacgac ttcgactacc tgcgctggac
     4561 cctcggcgag gtagagagag tctacgcccg cggcctgctg gggcgcgagt ataaccgcct
     4621 ggactacgtc ctcgttacgc tgcgcttcga gagcggcgcc atagcccacg tcgaggggca
     4681 ctgggggtac cccggtccct tcaactactc catcgaggtc gcggggagcg acgccctact
     4741 tactgtagat agcacagaac cagcctcgct ccagctgatc ggcggagccc ctgacgaagt
     4801 gcctgatctc gcctcgggca agagccctta cgagaaggaa ctcgaacact tcatccactg
     4861 tatcgcaaca ggagaagagc cgatcgtgca ggcgaaggac gcccgcgagg cactcaggat
     4921 cggcctggcc gcgacacaat ccgtcctaac tggcgaaccc atcactctgg gaggccggac
     4981 ttgagcgtca ggatcggtat cgtcagcttc gcccacgtcc actctccggc gtacgccgcc
     5041 gtgctacgcg atcttgccat tgcggatttc gtcggcatat ccgacgaaga tagagatcgc
     5101 ggcgccgagg ccgccgagct attcggcgtg cgcttcttcg ataacgtggc ctccctgatg
     5161 gacgaggtcg acgccgtcgt cgtctgttcc gagaacaaga accacgctcg ggatgtgatc
     5221 cccgcgctcc agggcggcgt acacgtgctc tgcgagaagc ccatcgcgac gaccgtcgaa
     5281 gacgcgaggg ccatgatccg ggcctccgag atctcgggca gccagctcag gacggccttc
     5341 cccgtgcgct acctcccctc ggtagtccgt gcgcgagaac tcgttcgcgg gggcgccctg
     5401 ggtcgcgttc tggccgtaaa cggcac
//
LOCUS       DATMVF010000142         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_566668, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000142 DATMVF010000000
VERSION     DATMVF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566668"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VN178_07195"
     CDS             <1..339
                     /locus_tag="VN178_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80796.1"
                     /translation="PHLSYVGDAEIGEDANLGAGTITANFDGQNKNRTTIGDGAFTGI
                     NTNLIAPVTIGEDAYTGAGSVVSKDVPPGKLAVGWPARAIKESPQSRRKRMREGDEAK
                     EQPRTTERED"
     gene            347..1339
                     /locus_tag="VN178_07200"
     CDS             347..1339
                     /locus_tag="VN178_07200"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWS80797.1"
                     /translation="MQNGYLTQTTEKRLMIVSGQGYPELSERIAERLDIELSGVELRE
                     FANGELYARYLESVRGADMFIVQSLCDPVNENLMQLLIMIDAAKRASAKRIVAVIPWY
                     AYSRQDRKTKPREPITARLIANMIQAAGAERVMTMDLHSGQIEGYFSFPVDHLTAMHT
                     FVDYFVDQGFLDAEDAVVVAPDTGEVKVAKQLAGHLRLPWAIVNKMRDKPGHSEVTHV
                     IGNVEGKRVVMIDDVVATGGTLVNAAKRLVDEGAVEVSAAATHGEFSGDAYRKIEDSP
                     LKEVVVTNTLPLKEDEPRSKIQTLNIAPILASTIKNVFTDESVSEVFMGENQLF"
     gene            1357..2085
                     /locus_tag="VN178_07205"
     CDS             1357..2085
                     /locus_tag="VN178_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWS80798.1"
                     /translation="MADNVELQAQERETRGKNAARRLRASGMTPAVLYGEGNEAGGST
                     ALAVPTKVVDYTLHHFGDNALYNIDFDGATSTARVVDAQRDPVSGVLVHVDFAPVDMS
                     ERIEVTVPIAVAGEAPGVEEGGVLQQVAYEIQVESLPGDIPQEITINVSGLGMHENLT
                     LGDLTLPEGVTLLSEPEDVAVTVTAPTEITEEEMEAAGIVEEPTEEELAAEEAAELEE
                     DAEGEEASEEDAAEEGPAEQNVQA"
     gene            2042..2668
                     /gene="pth"
                     /locus_tag="VN178_07210"
     CDS             2042..2668
                     /gene="pth"
                     /locus_tag="VN178_07210"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWS80799.1"
                     /translation="MLPKRVPRNRTSRLRLVRSFFEPDDEGGPIRSPFVVGLGNPGRS
                     YERTRHNAGYLVADELAKRHDGSWRKRKKAEAAPVALGLNNATLLKPTTFMNNVGSAV
                     SDHKPEDLIVVHDDLDLEAGTVRVKVGGGAGGHNGLRSIIGRVGNEFVRVRIGIGRPP
                     AGVTVTDYVLSRMDPAVKDAIPNAADAVEFLVEHGPEAAMNRFNVRTL"
     gene            2830..3615
                     /locus_tag="VN178_07215"
     CDS             2830..3615
                     /locus_tag="VN178_07215"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWS80800.1"
                     /translation="MGERVKAGYTVGRVADLSGVTIRTLHHYDEVGLLSPGGRSAAGY
                     RIYEDRDLERLQRILFYRELGFTLGEISTIVDDPRTDTLGHLRRQRGLLNERIERLSA
                     MVDAIDYEMEARTMDIPLTPEERLEVFGEFRPEDYAEEAEQRWGGTEAYRESQRRVSS
                     YTKEDWEKLKAEEEEIRARLTAALDAGLAPDSEEAMAAAEAHREHISRWFYECGYEIH
                     RGLTEMYVSDERFRSNYDTQAPGLAAFIRDAARANAGRAETGP"
     gene            3842..>4513
                     /locus_tag="VN178_07220"
     CDS             3842..>4513
                     /locus_tag="VN178_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS80801.1"
                     /translation="MGRRTAGLESAWTEVGGLRMHARLSTQDLPEGAPPIILVHGLGV
                     ASRSMVPLARALAPCYRVHAPDLPGFGDSERPPQVLNLAELTDYLVAWTRACRLERAV
                     FLGNSVGSQLVVRLAVRHPDLVERVVLQGPTMDPGARTMRQQTWRWIWNCRGERGSLT
                     PIALQEYWRCGLRPLLKTFRYALEDPIEEKLPSVRVPALVVRGSWDPVVTQRWAQEVA
                     DLLPVG"
BASE COUNT          902 a         1378 c         1527 g          706 t
ORIGIN      
        1 ccgcacctct cctacgtcgg cgacgcggag atcggggagg acgcgaacct gggcgccggc
       61 accatcacgg ccaacttcga cggccagaac aagaaccgca ccaccatagg cgacggggcg
      121 ttcaccggca taaacacgaa ccttatcgcg ccggttacaa taggggagga cgcctacacc
      181 ggggcgggaa gcgtggtcag caaggacgtg ccgccaggca agctcgccgt cgggtggccg
      241 gcgcgtgcga tcaaggaatc accgcagagc cgcaggaaga ggatgcgaga gggcgacgaa
      301 gcaaaggagc agccacggac taccgagagg gaggactagc gcggtcatgc agaacggcta
      361 tctgacgcag accacggaga agcggctgat gatagtcagc ggccagggct atccggaact
      421 atccgagcgt atcgccgaac ggctggacat agagttgagc ggggtcgagt tgagggagtt
      481 tgccaacggc gagctgtacg ccagatatct ggagagcgtc aggggagccg acatgttcat
      541 cgtgcagtcg ttgtgcgacc ctgtgaacga gaacctgatg caactcctta tcatgataga
      601 cgccgccaaa agggcctccg cgaaaaggat agtagccgtt atcccttggt acgcctactc
      661 gcgccaggac cgcaagacca aaccgcgcga gcccataaca gcgcggctta tagcgaacat
      721 gatccaggcc gcaggtgccg aacgggtgat gacgatggac ctgcactcgg gccagatcga
      781 gggctacttc tctttccccg tggatcacct gacggcgatg cacacgttcg tcgactattt
      841 cgtggaccag ggcttcctgg acgcggagga tgcggtcgtg gtcgcccccg acaccggcga
      901 ggtcaaggtc gccaagcagc tcgccggaca cctcaggctc ccctgggcca tagtgaacaa
      961 gatgcgcgat aagccgggac actccgaggt gacgcacgtc atagggaacg tagagggcaa
     1021 gcgggtcgtc atgatcgacg acgtagtagc cacgggtggg accctggtca acgccgcgaa
     1081 gcgcctggtc gacgaaggcg cggtggaggt cagcgccgcc gccacccacg gcgagttctc
     1141 gggggacgcc taccggaaga tcgaagactc acccctcaag gaggtcgtgg tgacgaacac
     1201 gctgcccttg aaggaggatg agccgcggtc taagatacaa acgctcaata tcgcaccgat
     1261 cctcgcgagc accatcaaga acgtcttcac ggacgagtcg gtgagcgagg tctttatggg
     1321 cgagaaccaa ctcttctaga ccgttaggag cgcgacgtgg cagacaacgt ggaactacag
     1381 gcgcaggagc gcgagacgcg gggcaagaac gcggcccgac gcctcagggc ttcgggaatg
     1441 acccccgccg tcctgtacgg cgagggcaac gaggccggtg gcagcacggc gctcgcggtc
     1501 ccgacgaagg tcgtggacta caccctccat cacttcgggg acaacgccct ctacaacata
     1561 gacttcgacg gggctacctc cacggcccgc gtcgtggacg cgcagcgcga ccctgtctcg
     1621 ggggtcctgg tccacgtcga cttcgcgccg gtagacatga gcgagcgcat cgaggtcacc
     1681 gtcccgatcg ccgtcgccgg cgaggcgccg ggcgtggagg aaggcggcgt gctgcagcag
     1741 gtcgcctacg agatccaggt cgagtccctc cccggcgaca taccgcagga gatcacgatc
     1801 aacgtctcgg gactcgggat gcacgagaac ctcacgctcg gcgaccttac gctaccggag
     1861 ggcgtgacgc ttctctccga gcccgaggat gtcgcggtta cggtcacggc gccgacggag
     1921 atcaccgagg aggagatgga ggccgcgggc atcgtcgagg agcccacgga agaggaactg
     1981 gccgccgagg aggccgccga gctcgaggag gatgccgagg gtgaagaggc gtccgaggag
     2041 gatgctgccg aagagggtcc cgcggaacag aacgtccagg cttaggctgg tccgctcttt
     2101 cttcgagcca gacgacgaag gggggccgat ccggtccccc ttcgtcgtcg gcctcggtaa
     2161 cccgggacgc tcctacgaac gcacccgcca caacgccggc tacctggtcg ccgacgagct
     2221 cgcgaagcgt cacgacggct catggcgcaa gaggaagaag gccgaagcag cccctgtcgc
     2281 cctcggcctc aacaacgcca ccctcctcaa gcctacgacg ttcatgaaca acgtgggctc
     2341 cgccgtctcg gaccacaagc cagaggacct catcgtcgtc cacgacgacc tcgacctgga
     2401 agcggggacg gtgcgcgtca aggtcggagg gggcgcgggc gggcacaacg gcctgcggtc
     2461 catcatagga cgagtgggca atgaattcgt ccgggtgcgc atcggcatcg gccgcccgcc
     2521 cgcaggagtc acggtcacgg actacgtgct ctcgcgcatg gacccggccg tgaaagacgc
     2581 catccccaat gccgccgacg cggtcgagtt cctggtcgag cacggcccag aagccgcgat
     2641 gaaccgcttc aacgtccgca ctttgtagcg gtcagcattc agccgtcagc tttcagctct
     2701 ttgtacttgc tgaccgctga tcgctgaaag cgcgagatgg gaagaaattc cttcccatct
     2761 cgctacagag atcccttgac cctcacgttg cgtgagggct tagactctcc tgtgcacagg
     2821 aggtgaacct tgggcgaacg agtgaaggca ggatacacgg tcggcagggt ggctgatctt
     2881 tcgggtgtga cgatccggac gttgcaccac tacgatgagg ttgggctgct atcgccgggc
     2941 ggacgtagtg cggccggcta ccgcatctac gaggaccggg atctggagcg gctgcagcgg
     3001 attctgttct acagggagct cgggttcacc ctgggtgaga tctcgaccat cgtcgacgat
     3061 ccgcgcaccg atacgctcgg gcacctacgc cgccagcgcg ggctgctgaa cgagaggata
     3121 gagcgcctga gcgcgatggt cgacgccatc gactacgaaa tggaggcaag gaccatggat
     3181 ataccactga cgccggagga acgcctcgag gtcttcgggg agttccggcc cgaggattac
     3241 gccgaagagg ccgagcagcg ctggggcggg accgaggcgt acagggagtc tcagcggcga
     3301 gtatctagct acacaaagga agactgggaa aagctcaagg ccgaggagga agagatacgc
     3361 gcccgcctga ccgcggcgtt ggatgccggc ctggcacccg atagcgagga ggccatggcg
     3421 gcggccgagg cccaccgcga gcacatcagc cgctggttct acgagtgcgg ctacgagatc
     3481 caccgcggcc tgacggagat gtatgtcagc gacgaaagat tccgctccaa ctatgatacg
     3541 caggcccctg gcctggccgc ctttatcagg gatgcggccc gtgccaacgc cggccgggcc
     3601 gagaccggtc cctagtgcaa gatccacgtc cctttggagt acggcatcta gatcccgtag
     3661 cgggagagga ctcttagccc gcgctcgcct ttggcgagcg tcgatggagg caccttcttg
     3721 gtggggcagg cgactttgcc tgccccaccg gcactccgtt gagagtactt gtggccggaa
     3781 ggctgatagt attgccgagg tcggtcgtaa atgaggtgcg cgtcagcgag aacggagatg
     3841 tgtggggcgg cgaacggcag gactagagag cgcgtggacc gaggtcggag gcttgaggat
     3901 gcatgctcgc ctctccacac aagacttgcc cgagggcgct ccgccgataa tcctggtgca
     3961 cgggcttggg gtcgcgagca ggtccatggt ccccctcgcc agggctttgg ccccatgcta
     4021 ccgcgtccac gctcctgacc tgcccggctt cggagatagc gaaagaccac cgcaggtact
     4081 gaatctggcc gagctcaccg attatctggt tgcctggacg cgcgcatgcc ggctggagcg
     4141 tgccgttttc ctgggtaact ctgtcggtag ccagctggtg gtgcgcctgg cggttcggca
     4201 tccggatctc gtcgagcgcg tagtgttgca ggggccgacc atggacccgg gggcacgcac
     4261 gatgcggcag cagacctggc gttggatatg gaattgccgc ggcgagcgcg gctcgctgac
     4321 gccgatcgcg ctgcaagaat attggcgttg cggtcttcgg cccctcctga aaaccttccg
     4381 gtatgccctc gaggatccca tcgaggagaa gctccccagc gttcgggtac ccgctttggt
     4441 ggtgcgggga tcctgggatc cggtcgttac gcagcgctgg gcgcaggaag tggcggacct
     4501 cctgccggtg ggc
//
LOCUS       DATMVF010000143        20002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_611002, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000143 DATMVF010000000
VERSION     DATMVF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 20002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20002
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611002"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VN178_07225"
     CDS             complement(<1..481)
                     /locus_tag="VN178_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWS80802.1"
                     /translation="MLDAATNYLAVIKVVGIGGGGTNAVNRMINSGLQGVEFIAINTD
                     AQALQMCDADQKIHIGEKITRGLGAGADPKIGMEAAEENKAEIEEAMRGADMVFVTAG
                     KGGGTGTGAAPVVAKIARDSGALTVGVVTRPFAFEGRRRSTYAEEGIKRLKENVDSLI
                     "
     gene            complement(525..1727)
                     /gene="ftsA"
                     /locus_tag="VN178_07230"
     CDS             complement(525..1727)
                     /gene="ftsA"
                     /locus_tag="VN178_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWS80803.1"
                     /translation="MAQTLAYGIDVGTTKVVAIAGRVDPRRGWVEVLSMGEAPSYGLK
                     RGVVVDRKATADSIAEAMEACGVRGGRVTVGIAGSHISSFNTEVTLLNRSRDLTVTDR
                     FVRKLEQEARQLELHEDERVIHVVPRGYVLDGSEGVKNPEGLAARKVTMRAHVVSGAL
                     SSIQNLLRAVEDCGVEVSRIVLEPLASAEACLLDSDRESGIVLLDMGGGTTDIATFYR
                     GALTHTDVIPIGGESFTSDVAYGLKIPQHHAEELKLRYGTVLSDTVDPVAAVKLDDRH
                     YNASFVSQILEYRGREVLDYVRDSLDRTMSRELVPAGVALTGGGSLLDGMTGLTEEVL
                     GLRAKTVSPRRVRGEVEPVQKPQYATAVGLLYFSARNDEPRPKGKGAGAHSFGSILEA
                     VKGWFRGR"
     gene            complement(1734..2108)
                     /locus_tag="VN178_07235"
     CDS             complement(1734..2108)
                     /locus_tag="VN178_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80804.1"
                     /translation="MLDADVDGRRFVLSADGEELPGPGGAGLRRVELDRDQVGEILVF
                     ARTLYGSGLTLESVDGAGAAGIEATVEGRAVIFSGNVGERRVTALKHIMPEHPDARVF
                     DLRSPERVVVGVPHETRREPRG"
     gene            complement(2402..3766)
                     /gene="murC"
                     /locus_tag="VN178_07240"
     CDS             complement(2402..3766)
                     /gene="murC"
                     /locus_tag="VN178_07240"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWS80805.1"
                     /translation="MNIHMIGIGGAGMSGIAEVLARRGHSVTGSDLKESPYTRRLREA
                     GVTVYIGHAPDQVGDADRVVISTAIPTTNPELLQARRRSIPVIPRAAALAWILQGGRS
                     VAVAGTHGKTTTTSMTAHVLEALGESPTALIGGELNDIGSNVAFGRSDLVVAEADESD
                     RSFLYLRPQAAVVTNIEFDHPDFYSSLDDVVETFEQFVHSLPEDGHLVVCADDPCCSL
                     VAAGAPCSVTTYGIGSGDLRADLASPSSYVLFEDGHRRGEVELGVYGRHNVLNSLAAA
                     AVARWLGHGAFEAASTLRNFGGVRRRFQLKGEQADIRVVDDYAHHPTELSATLAAARS
                     TMPGDGRVIAVFQPHRYSRTRALYREFGASFSSADAVLVTEVYGAGEMPQPGVNGKLI
                     VDAICETRGHPEVYYIPQQDAIPAVLQSVSRRGDLVLTLGAGDISKAGEDLLGMLEER
                     GSKP"
     gene            complement(3763..4851)
                     /gene="murG"
                     /locus_tag="VN178_07245"
     CDS             complement(3763..4851)
                     /gene="murG"
                     /locus_tag="VN178_07245"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWS80806.1"
                     /translation="MNEQERSNTPRILIAGGGTGGHAIPALCVADELRSRGAIVEFVG
                     SSTGIEISLVPRQGYTLHALPLTGLLGGPLAQARAAFLSLNAVTRCRRIIRRLRPLSV
                     LGVGGYASAPAVIAARTLGVPTFLHEQNSVPGRVNRLASRFTTQTLATFPEATGRLGN
                     AVWVGMPTRTEIFEASREEALRDLGLEPPVVLVFGGSGGALRINLAAAEAFGGATSYT
                     VVQISGRRDFSRLRTNNPRHTILEFVPDIWRYLAAADLVVSRAGAGSLFDIAAAGKAA
                     ILIPFPYATGDHQLENAKYFTGGGAAELMLDSAVGARELRDRVEELLDDDVRREELGR
                     RMGALATPGAAGEVARRLLRAGQKEFGA"
     gene            complement(4757..5896)
                     /locus_tag="VN178_07250"
     CDS             complement(4757..5896)
                     /locus_tag="VN178_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HWS80807.1"
                     /translation="MSSLRANLFLVALGLSMLGLVMVYSATYSEVGTDFLFVRIGHTA
                     LGVATFFVASRVRYTSWRRYAPALYLIVLLGLILVLIPGIGTQINGARRWLDLGPMSI
                     QPAEFAKLAAILVLSCAVARLPHGSGLPFKPLVAVGVLFILVFVEPDFGTSVVLLAGV
                     AGTLWASELRTRDLVLSGLVGLVAVAGMMVLEPYRRERFLTFLDPWAAADGSGYQVVQ
                     AMVAIKAGGFFGSGAGSGGQGTYVPELGTDMIFALIGEELGLIGMVVVIAAFGFIALA
                     GLGIAMNAPSVMARCVAAGLTTMLIAQATFNIGAAMVVLPLAGMTLPFVSYGGSSLLV
                     CFAAVGILYRISEDSERAREIKHTANPDRRRRHGRARYPRAVRGG"
     gene            complement(5893..7224)
                     /gene="murD"
                     /locus_tag="VN178_07255"
     CDS             complement(5893..7224)
                     /gene="murD"
                     /locus_tag="VN178_07255"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWS80808.1"
                     /translation="MRTLVYGLGESGVAATRALTERGEVVLVADGSDDERLRDTLAAL
                     DVPGILGAGSGVLEGIDRIVASPGISPRNPVLRAAGARGIPIVSEVGLGLELLGERAR
                     VAAVTGTNGKTTVVDMLHRMLEASGVPHAVAGNSWRSLTGCVDEARTAGLLVLEVSSF
                     QLHYLQSPGFEVAALLNVRQDHLNWHSSFEEYSADKLRIFEGQRAEDLALVSATDPIG
                     RAAIGTIKAETLVVGEGETALRDGRLLLCGSHLADVSELRFAGTHNHENALFAAAAAQ
                     RLGAELGEIRATLLEYESKPHRMRVAAQISGVTYVDDSKATNPAAVAAALESFDVPVV
                     LILGGSEKNTDFSEVLPDLGVCRAVVCQGEAGPRIAAYLEDEGFQDQVHRSPDLEKAV
                     ERATELARPGDVVLLSPGCASFDQFPGYAERGDAFVGFARDLAGHHGTVGR"
     gene            complement(7229..8224)
                     /gene="mraY"
                     /locus_tag="VN178_07260"
     CDS             complement(7229..8224)
                     /gene="mraY"
                     /locus_tag="VN178_07260"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWS80809.1"
                     /translation="MFSVVLAAAVAFLVTVLLGSKYIEFLQSRKFGQFVREEGPQTHL
                     IKAGTPTMGGIVMLTGLLGALLVVARPNVATLTTLLLVAVVAGIGLYDDWQKVSKKRP
                     EGLSARYKFLLLTLAVVLADVLAVRYVGVTQTVLVPYFDRSLVLGPGVLGIALFSVFL
                     LLVIAGTTNAVNLTDGLDGLAAGAAAIALLAYTAIAFLERQYDVAVICGAMVGAIVGF
                     LWYNSHPADIFMGDTGSLAIGGILAAAAVLTKTELLLPIVGGLFVVEALSVIVQVLVF
                     KLTGRRVFKMAPIHHHFEFLGWDENKVVVRFWIAQAAFSSVGFFLYYIFLYNTAG"
     gene            complement(8217..9587)
                     /gene="murF"
                     /locus_tag="VN178_07265"
     CDS             complement(8217..9587)
                     /gene="murF"
                     /locus_tag="VN178_07265"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWS80810.1"
                     /translation="MRPATLSEAARAMDAELRTTHVNTLVGGAATDSRKVREGDLFFA
                     LRGRTDGADFAPEAHLRGAVATVATRRLDVPTLVVDDPLEALQRLARWTLVRGDAPSP
                     TVVGITGTVGKTTTKDALATILRHAGRRVSATEGNLNNEIGLPLTILASDPRTEVLVL
                     EMGATHAGDIQYLCAIAPPDIGILTAISPVHLDSFGTLEDLAAAKGELALSLPESGTL
                     IAPAGAPEAATGSGRSLERITFARAGTADLVASEIEGTEEGLRFVASIGGESAEVRSP
                     VFGTHLVEPLLAALGGALALGLDLEQAARGLSRLRRTGLRGDLYSLKEDIIVYDDSYN
                     ASPAAVAAVLAYGADQARRQRRRLVVVLGGMFELGACARAYHREAGELAAQVGVDLLV
                     CVGDEARWYAETFPGRTIFYPDAESAADALRTELRARDYLVVKGSRGVGLDTLTRKLR
                     ESLALV"
     gene            complement(9584..11185)
                     /locus_tag="VN178_07270"
     CDS             complement(9584..11185)
                     /locus_tag="VN178_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWS80811.1"
                     /translation="MVAAVFVVTGLLLGGRAVHISLTEDEDYRAFADEQAGGTTPVVA
                     PTRGSIVSADGRELATSLEVARVIATPYQIEDPGATARELHAELGEETDTTEKQIRTS
                     LTRRGVDGQLSGYSVVATGVEPKTAARVQAMGIEGITTTPDTMRVYPDGSLASQLLGH
                     QGDYGEPYGGVEASYDDTLKRGGDVDLTVDSAVQQVLQKALAKAVAESKARSAVGVVM
                     RVDDGSILALANTPAYDNNEFGDVPPEYQRDRVLTDPYEPGSTFKAFTVASALEEGAV
                     TPSSEFVVPDHMTVADHVIHDSQPHETEVMTPKEVLEHSSNVGVTKVAKELGGKQLYD
                     YMRAFGFGKPTGVDLWGEDLGIVPAYKDWSGVSIGNIPFGQGFTVTPLQLVSGYATLV
                     NGGRRVTPHVTQQQSPEGQGPRVISKKTSAIVRGMLQGVVDDGSGHFAQISGYTVAGK
                     TGTSQIVDPKTGTYGDQYVASFVGFAPAADPEYVMLVAVDDPQTSYWGELVAVPAFRQ
                     VMSFTLGYFNVPPDRRGFVAEEPLW"
     gene            complement(11403..11828)
                     /locus_tag="VN178_07275"
     CDS             complement(11403..11828)
                     /locus_tag="VN178_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80812.1"
                     /translation="MAASQRRYKATLPTEPAWPVQKRAKDGPRDRSVRRKAAGRRRRF
                     AMLVVVPVLLMLGSVYLNTVAAGLDVRAAELDVRLDRAREVGERLEVRVAELSGADRI
                     RALAAEKLGMHEPKSTTLRVYGAEDGENDGVPQEGGRPR"
     gene            complement(11828..12763)
                     /gene="rsmH"
                     /locus_tag="VN178_07280"
     CDS             complement(11828..12763)
                     /gene="rsmH"
                     /locus_tag="VN178_07280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWS80813.1"
                     /translation="MATGHRPVMLERAVAALGLAPEDTVVDATFGGGGHSRLILARLG
                     PDGRLLGIDRDPEAAGRASDLLEDPRFEFVSGAYDVALWAMVEEGRGADAILFDLGLS
                     SFQVDEPARGFSYVGEGPLDMRMDPRSGMTAADFLNTVDVGELSRVLSEYGDVPRAEA
                     GRVAREVVRRRPLAVTTDLYEAVRAAVGWKQRGGNPAKRIFQAVRVRVNDELGSLGRA
                     LEASERLLVPGGRLVVITFHSGEDRLVKRFISGREGRCVCPPELPVCVCGAKPIFRRG
                     EVLTPSRREIEENPRSASARLRAAIRTAEPPVDDS"
     gene            complement(12763..13203)
                     /gene="mraZ"
                     /locus_tag="VN178_07285"
     CDS             complement(12763..13203)
                     /gene="mraZ"
                     /locus_tag="VN178_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWS80814.1"
                     /translation="MALLGEYEHTLDEKGRMTLPSRLRSYFEGGIVVTKGVDRCLFAF
                     PPEEWATFKANIKANADLSARGRQLSRMFFSMAFEATLDRQGRVLIPTKLASYAGLRR
                     EVTITGVDDRLEIWDTEEWDRYVAGADDTFSEIVEEFAGRDLGG"
     gene            complement(13521..13594)
                     /locus_tag="VN178_07290"
     tRNA            complement(13521..13594)
                     /locus_tag="VN178_07290"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13558..13560),aa:Ile,seq:cat)
     gene            complement(13620..13692)
                     /locus_tag="VN178_07295"
     tRNA            complement(13620..13692)
                     /locus_tag="VN178_07295"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13657..13659),aa:Asn,seq:gtt)
     gene            complement(13733..15496)
                     /gene="dnaG"
                     /locus_tag="VN178_07300"
     CDS             complement(13733..15496)
                     /gene="dnaG"
                     /locus_tag="VN178_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWS80815.1"
                     /translation="MRISQRSIEQVKETADIVEVASEFTALKRQGTRFTGLCPYPDHQ
                     EKTPSFSLSPDQGLYYCHGCQRGGDAIKLAMDLKSLDFAEAVSYLADRSGVDLQFEGG
                     GDPDAARERNLRRRAIHKALAAATVYYHKYLLKSKTPEAQRARRYLKDRRIDSSTIEE
                     FRLGYAPPRGVSGFVAAAGRLDLDRSVLDAAGLLSTRGGERFAGRVTFPISDRRGRIV
                     GFGARTLGDAQPKYLNSPETEIFNKRDLLYGFPQVSEAIRRERSALVVEGYTDVLMLY
                     QSGIENAVATLGTATTPGHLRMLSRYADRIYVLFDPDAAGERAIERAAATAADLKLDL
                     RVLRLPEDPADWLLEHSPEEFARKLNDSVPVLEYGIRRIVARTRGADATERSRAVPEV
                     RNMIQEIKDPVLYREAVRLATESLGVGPDVLQAAQPPTAPAGSSSRDRGPTDPQMEAG
                     AAVLTLILARPDLTATFLQEGIEIPGLPEPLTLQGEDFRDETHAKIFTVLRDHAGRDL
                     DAVLSDPRVRPMMDQIGALASQGERLYPSETSVREAWLRLAILSREHSKRETPDYDRK
                     DVLHSEIQHLKQALRSVSAGA"
     gene            15900..16784
                     /locus_tag="VN178_07305"
     CDS             15900..16784
                     /locus_tag="VN178_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80816.1"
                     /translation="MNRLYILLTVMVVMVGALFTGHQALAEVLAGTEGNDTIVGTNRD
                     DRLEGLGGDDTIKGRRGDDEIFPGEGDDDVFAGAGDDRIFARDTAGRDYIDCGSGFDV
                     VETIHRDDRTLRNCERAPGPSRDVGGTTGTTDPRTTGTTTGTTTGTTTGTTTGTTTGT
                     TGTTGTTTGRTRSTTTGTTGTTTGTTGITSRRTTSTTGTTTGTRDTTGTTGASTGTTG
                     GDTTGATTRDTTGANTTGAATTGDRNSPRDDVIRDTIPQGGELPNTGGPSGLVPAVAL
                     LALLINGAVIGLLFVRRR"
     gene            complement(16879..17865)
                     /locus_tag="VN178_07310"
     CDS             complement(16879..17865)
                     /locus_tag="VN178_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWS80817.1"
                     /translation="MRSQGPGVGLRHDPSTWAVEHSAGRPRPESPDGVRNDFQRDRDR
                     IIHAKAFRRLMHKTQVFIAPDGDHFRTRLTHTLEMMQISRTIARALGLNEDLTEAVSL
                     GHDLGHTPFGHTGEEALSRALTAHGRTFRHNEHSLRVIDHLENQGDGLNLTFEVRDGI
                     LNHTGTGYPRTREGEIVRVADRIAYVNHDVDDALRAGIISSDDLPPNPISVLGEQMSA
                     RIDTLVRDMISNSKMKGEVSLSDPVYEALMELRTWLFRHVYDNELGSRENQKAARVVT
                     DLFWYYLERPEERVASDPDPLTETIDFVAGMTDRYALATYRRLFLPRGEIFA"
     gene            complement(17862..18614)
                     /gene="recO"
                     /locus_tag="VN178_07315"
     CDS             complement(17862..18614)
                     /gene="recO"
                     /locus_tag="VN178_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWS80818.1"
                     /translation="MAVYKSKGIVLRSIRYSEADRILDLYTTEAGLVSAIAKGIRRTK
                     SRFGARLEPLSCVEFVAYHGRTLDTVTQAETLRSFHGIREDLARFETAAGMVGSVRAL
                     SGGDEADRRVFNLLFNALHTLETSDSGFENIEAALGIKLSILSGYAPQLDVCLACATN
                     LDAASEPLHFAPDHGGVLCQECRPATGDAFPLAPGTLESLRILVESPIRQAPAQTGPE
                     GSVLRVVRSHVLAHAPGNTLAGPRLAGAGRRA"
     gene            complement(18595..19296)
                     /gene="deoC"
                     /locus_tag="VN178_07320"
     CDS             complement(18595..19296)
                     /gene="deoC"
                     /locus_tag="VN178_07320"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWS80819.1"
                     /translation="MRVREFAKTVDHTLLKPGATEQEIVSLCKEAAHYHFAAVCIPPS
                     YVPLAARELRGADVKVATVISFPFGYESTAVKIAAVREAVTRGASELDVVMNISKFLS
                     GDFGYVADELASLTQEVSAVAMNNGLNDVVVKVIVETAYLSDKMKRLATQIVAASGAD
                     FVKTSTGFAPAGATPEDVALLREEAPEGLAVKASGGISSLGDALDILNAGASRLGMSR
                     SVAVIKEAEGGGLQE"
     gene            complement(19337..>20002)
                     /gene="era"
                     /locus_tag="VN178_07325"
     CDS             complement(19337..>20002)
                     /gene="era"
                     /locus_tag="VN178_07325"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWS80820.1"
                     /translation="EQVVDSLSESDVILLLFDAPQASEGLGSGDRYIARLVAGSGTPA
                     IVTINKTDLLRTRESALPIVQAVSRLGDWNEVFQISAANGTNVDPLMDSVVGLLPHGP
                     RYFPEGTVTDYPESLVLAEYVREKALNVLREEVPHAVAVEIEDVERKENVTVVYAVIH
                     VERATQRMIVLGKGGKTIKRIGTEARRDVERLLGTRIYLDLKVKVSQGWRSDRQFLER
                     LGL"
BASE COUNT         3512 a         6513 c         6364 g         3613 t
ORIGIN      
        1 tgatgaggga gtcgacgttc tccttgaggc gcttgatgcc ctcctcggcg tacgtggaac
       61 gcctgcggcc ctcgaaggcg aaaggccgcg tgacgacccc gacggtcagc gcccccgagt
      121 ccctggctat cttggcgacc acaggggcgg cgcccgtgcc tgtcccgccg cccttgcccg
      181 cggtgacgaa gaccatgtcg gcgccacgca tcgcctcctc gatctccgcc ttgttctcct
      241 ccgcggcctc catcccgatc ttcgggtccg cgcccgcgcc gagtccgcgt gtgatcttct
      301 cccctatgtg gatcttctgg tcggcatcac acatctggag agcctgggca tccgtgttta
      361 tcgctatgaa ctcaaccccc tgcagtccgg agttgatcat gcggttcacg gcgttggtcc
      421 cccctccgcc aatgccgaca actttaatga ccgccagata attagtcgcc gcgtccaaca
      481 tgcctactta accctccatc acacgttcgt cgaggcccac gggtctacct cccccggaac
      541 cagcccttga ccgcctccaa tatgctacca aaactatgcg cccctgcacc tttacccttt
      601 ggtctaggtt catcgtttct ggccgagaag tataacagac ccaccgccgt tgcatactga
      661 ggtttctgta caggttcaac ctcgccccta acgcggcgag ggcttacggt cttagcgcgc
      721 aaaccgagga cttcttctgt aagccccgtc atcccgtcga gcagggagcc gcctccggta
      781 agggcgacgc cggctggaac gagttccctc gacatggtcc gatccaggga gtcgcgcacg
      841 tagtcgagca cttcgcggcc gcggtactcc aggatctggc taacgaagct cgcgttgtag
      901 tggcggtcgt cgagcttgac ggcggccacg gggtcgacgg tgtcggagag gaccgtaccg
      961 tagcgaagtt tgagctcctc ggcatggtgt tgcgggatct tgaggccgta ggcgacatct
     1021 gaggtgaagc tctctccgcc gatggggatg acgtctgtat gggtgagagc acccctgtag
     1081 aaggttgcga tgtcggtcgt gcctccgccc atatccagca ggacgatgcc ggattcgcgg
     1141 tcggagtcga gcaggcaggc ttcggccgat gccagtggct ctagcacgat cctggagacc
     1201 tctacgccgc agtcttcgac ggcgcgcagg aggttctgga tgctggagag cgccccgctc
     1261 acaacgtgcg cgcgcatggt caccttgcgg gcggcgaggc cttccgggtt cttgacgccc
     1321 tcggacccat cgagcacgta gccccgaggg acgacgtgta tcacccgctc gtcctcgtgc
     1381 agttcgagct ggcgggcctc ctgttcgagc tttcggacga atctgtccgt gaccgtcagg
     1441 tccctgctgc ggttcaggag cgtgacctct gtgttgaacg aagagatgtg gctaccggcg
     1501 ataccgacgg tcaccctgcc gccgcgaaca ccacaagctt ccatggcttc ggcgatcgaa
     1561 tcggcggtgg ctttgcggtc taccacaacc ccccgcttga gtccgtagct cggcgcctcc
     1621 cccatggata ggacttcgac ccaacctctt cgcgggtcca cccggccggc gatcgccact
     1681 actttggtgg tacccacgtc tatcccgtag gctagcgtct gcgccacggt cgactagccc
     1741 cttggctctc tgcgcgtttc gtgcgggacg ccgacgacca ccctctccgg cgaacgcagg
     1801 tcgaagaccc tggcgtcggg atgctccggc atgatgtgtt tcaaggccgt gacccgtcgt
     1861 tcaccaacat tcccagagaa gatgaccgcg cggccttcca ccgtggcctc gatgccagcg
     1921 gcaccagccc cgtcgacaga ctccagcgtc aacccgctcc cgtacaaggt cctcgcgaac
     1981 accaagatct cccctacctg gtcgcgatcc aactccaccc tccgaagacc agctccccct
     2041 ggccccggaa gctcctcgcc gtcggccgag agaacgaacc tccgcccgtc cacgtcggca
     2101 tccaacacag gcctacgctc ctcaacctga acggtaacta taccggagtt ccaatccacg
     2161 ttcacctcca caccctgaac ccaaggatga gacttcaggt tgcgcttcaa catttcttcg
     2221 ttgagtgtga ggaggctggc gtggtctgga actgcccctg tgacttcgga ttcggggtac
     2281 atcctggcgc cttccaccct gacgccggtg acggggaaag cgaggtacgt agcgaggatg
     2341 aggatgacgg tagtagcggc ggccgcggcg accgagagca ggatggcgcg cagggatgtt
     2401 ctcagggttt ggatcctcgc tcctccagca tgccgagaag gtcttcgccg gctttcgaga
     2461 tgtcacccgc gccgagggtt agcacgaggt ctccccttct ggagacgctt tgcagcacgg
     2521 ctggtatggc atcttgctgg ggtatgtagt agacctcggg gtggccccgc gtctcgcaaa
     2581 tggcgtcgac tatcagtttg ccgttgacgc ccggttgggg catctcaccg gcgccgtaga
     2641 cctcggtgac gagcacggcg tcggccgagg agaaagaggc gccgaactcc ctgtagaggg
     2701 cgcgggtacg cgagtaacgg tggggctgga agacggcgat cacccttcca tcccctggca
     2761 tggtggaccg ggcggcggcg agcgtggccg agagctcggt aggatggtgg gcgtagtcgt
     2821 cgacgacgcg tatgtctgcc tgctctcctt tgagctggaa gcggcggcgc acgcccccga
     2881 agtttcggag ggtgctggcc gcctcgaaag ccccgtggcc gagccagcgg gcgaccgcgg
     2941 ccgccgccag ggagttgagc acgttgtggc gcccgtagac gcccagttcg acctcgccgc
     3001 gccggtggcc gtcctcgaag agcacgtagc tgctcgggct cgccaggtcg gccctgagat
     3061 ctccagagcc gatgccgtaa gtggttacgg agcatggcgc cccggcggcc accagggagc
     3121 aacaagggtc atccgcacag acgaccaggt ggccgtcctc gggcagagag tggacgaatt
     3181 gctcgaaggt ctccacgacg tcgtcgaggg aggagtagaa gtccgggtgg tcgaactcga
     3241 tgttcgtaac cacggcggcc tgtggcctga ggtagaggaa agaacggtcg ctctcgtccg
     3301 cctcggccac gacgaggtcg gacctcccga aggcgacatt gctgccgata tcgttcagtt
     3361 cgccgcctat cagggccgtc gggctctcac ccagagcttc gaggacgtgc gccgtcatgc
     3421 tcgtcgtcgt cgtcttgccg tgggttcctg ccacggcgac gctgcggccg ccctggagga
     3481 tccaggcgag cgctgcggcg cgtgggataa ccgggatcga cctgcgcctg gcttgcagga
     3541 gctccgggtt cgtcgttggt atggccgtcg agatcacgac acggtctgcg tcacctacct
     3601 ggtcaggggc gtgcccgatg tagacggtga cgcccgcctc acgcagcctg cgggtgtaag
     3661 gagactcctt gagatccgag cccgtgacgc tgtgacctct tctggccagg acctcggcga
     3721 tcccactcat acccgccccg ccgatgccga tcatgtgtat gttcatgcgc caaactcctt
     3781 ctgtccggca cgcagcaatc gcctggctac ttcacccgcc gcacctggcg tagcgagcgc
     3841 gcccatgcgc cggccaagct cctcccgcct tacatcatcg tccaacaact cttcgacgcg
     3901 gtcgcgaagt tcacgcgcgc cgaccgcgga gtctagcatc aactccgcgg ccccaccgcc
     3961 ggtaaagtat ttggcgttct cgagctggtg atctcccgtc gcgtagggga aggggatgag
     4021 gatcgccgcc ttccctgcgg ccgcaatgtc gaacagagag cccgcgccgg ccctgctaac
     4081 gaccaggtcc gcggcggcca ggtaccgcca gatgtccggg acgaactcga ggatcgtgtg
     4141 cctgggattg ttcgtccgta gccgcgagaa atctctgcgg ccggagatct ggacgaccgt
     4201 atacgacgtc gcgccgccga acgcctccgc cgccgccagg ttgatcctga gcgcaccccc
     4261 gctcccgccg aagacgagta cgaccggcgg ctccaggccg agatcccgca gcgcttcctc
     4321 ccgtgaagcc tcgaagatct cggttctggt aggcataccg acccacacgg cgttcccgag
     4381 ccgccccgtg gcttcgggga acgtggccaa cgtctgcgtc gtaaagcggc tggccaggcg
     4441 gttgacccgc ccggggacag agttctgctc gtgcaagaac gtgggcacgc ccagcgtcct
     4501 ggccgcgatc accgccggcg cgctcgcgta gcccccgacg ccgagaacgg acaacggcct
     4561 cagccgtcgt attatgcgtc tacaacgggt gacggcgttc agggagagga aggcggcgcg
     4621 ggcctgggcc agcgggccgc ccaggaggcc ggtgaggggc agggcgtgca gggtgtagcc
     4681 ttgcctgggt acgagggata tttcgatgcc tgtgctggat cccacgaact cgactattgc
     4741 tcctctcgat ctcaattcat ccgccacgca cagcgcgggg atagcgtgcc ctcccgtgcc
     4801 gcctcctgcg atcaggattc gcggtgtgtt tgatctctct tgctcgttca ctgtcctccg
     4861 aaatcctgta gaggataccc acggcggcga agcataccag cagactcgaa ccaccgtagc
     4921 tgacgaatgg gagcgtcatc cctgcgaggg ggagaacgac catggccgcc ccgatgttga
     4981 aggtggcctg ggcgatgagc atcgtagtca ggcccgcggc cacgcaccgg gccatcaccg
     5041 acggggcgtt catggctatc cccagcccgg cgagggcgat aaagccgaag gcggcgatca
     5101 cgacgaccat cccgatcagg ccgagttcct ctcctatcag ggcgaagatc atatccgtgc
     5161 ccagctcggg gacgtatgtt ccctgtccgc ccgatcccgc ccccgaaccg aagaagcccc
     5221 cggcctttat ggccaccatg gcctggacta cctggtagcc ggacccgtcc gcggcggccc
     5281 agggatcgag gaacgtgagg aaccgctcgc ggcggtaggg ctcgaggacc atcatccctg
     5341 ctaccgccac caacccgacg agcccggaca gcaccaggtc cctggtcctc agctcggagg
     5401 cccacagcgt tcccgccacg cccgcgagca ggaccacgga ggtgccgaag tccggttcga
     5461 cgaagacgag gatgaagagg accccgacgg caacgagggg cttgaaggga agccccgaac
     5521 catgaggcag acgcgcgacc gcgcagctca agaccagaat ggcggcgagc ttcgcgaact
     5581 ccgcgggctg gatgctcatg gggccgaggt cgagccagcg gcgggcgccg ttgatctggg
     5641 ttcctattcc gggtatgagg acgaggatta ggccgagaag cacgatcagg tacagggcag
     5701 gggcatacct gcgccacgat gtatacctga cccggctggc cacgaagaag gtggcgacgc
     5761 cgagtgccgt atggccgatg cggacgaaca gaaaatcggt cccgacctca ctgtacgtag
     5821 ccgaatagac catgacgagc cccaacatgg agaggccgag cgccaccagg aacaagttgg
     5881 ccctcaggct actcatcgtc cgacggtccc atgatgtccg gcgaggtccc tggcgaaccc
     5941 gacgaacgcg tctcccctct cggcgtatcc ggggaactgg tcgaagctcg cgcacccggg
     6001 cgagagcagg acgacgtcgc ccggcctggc gagctctgtg gctctttcga cggctttctc
     6061 caggtcgggg ctcctgtgga cctggtcctg gaagccctcg tcctccaggt aggcggcgat
     6121 gcgtgggcca gcctcgccct ggcacaccac cgcccggcac actcccaggt cgggcagcac
     6181 ctccgagaag tccgtgttct tctccgagcc accgaggatc agcactaccg gaacgtcgaa
     6241 gctctccaag gcagccgcca ccgcggccgg gttggtggcc ttcgaatcgt ccacgtaggt
     6301 cacgcccgag atctgtgccg ctacccgcat gcggtggggc ttggactcgt attcgagcag
     6361 cgttgccctg atctccccga gttcggcacc cagcctctgt gcggcggccg ccgcgaagag
     6421 cgcgttctcg tggttgtgcg tccccgcgaa acgcagctcg gacacgtccg ccaggtgaga
     6481 gccgcagagg agtagacgcc cgtcacgcag ggccgtttcg ccttcgccga cgacgagggt
     6541 ttcggccttg atggtgccga tagcggcccg gcctatgggg tctgtcgcgc tcacgagtgc
     6601 gaggtcttct gcccgctgac cctcgaagat acggagcttg tcggctgagt actcctcgaa
     6661 cgaggagtgc cagttcaggt gatcctgcct cacgttgagg agcgccgcga cttcgaaccc
     6721 agggctctgg agatagtgga gctggaagga agagacctcg aggacgagca gccccgcagt
     6781 acgtgcctca tcgacacagc ccgtcaggct tctccaggag ttgccggcga cggcgtgcgg
     6841 cacgccggag gcctcgagca tgcggtggag catgtcgact acggtggtct ttccgttggt
     6901 gccggtgacg gccgctaccc gcgctcgttc gccgagcagt tccagcccga gcccgacttc
     6961 cgagacgatc gggatgcccc gcgccccggc tgctcgtaag accggattgc gaggactgat
     7021 gccggggctc gctacgattc tatctatacc ttccagtacc ccagatccgg ccccgaggat
     7081 gcctggcacg tcgagggctg ccagggtgtc tcgcagcctc tcgtcgtcac ttccatcggc
     7141 caccaggact acttcgcctc tttcggtgag ggcccgcgtg gccgcgacgc ccgattctcc
     7201 gagcccgtat accagggtcc tcacgggctc atcccgccgt gttgtagagg aagatgtagt
     7261 agaggaagaa ccccacggag gagaaagcgg cttgggcgat ccagaatcgg actaccacct
     7321 tgttctcgtc ccagcccaga aactcgaagt ggtggtggat cggggccatc ttgaagaccc
     7381 ttcgtcctgt aagcttgaag accaagacct ggacgatgac ggagagggct tcgacgacga
     7441 acagaccgcc cacgatgggc aggaggagct ccgtcttggt gagcacggcc gccgccgcga
     7501 gaatcccgcc gatcgcgagc gagccggtat ctcccataaa gatgtcggcc gggtgagagt
     7561 tgtaccagag gaacccgacg atcgccccga ccatcgcgcc gcagatcacg gcgacgtcgt
     7621 actggcgctc caggaaagcg atggccgtgt aggcgagcag ggctatggcc gccgccccag
     7681 cggcgagccc gtcgaggccg tccgtcaggt ttacggcgtt ggtcgtgccg gcgatcacca
     7741 gcagcaagaa cacgctgaag agcgcgatcc ccaggactcc tggccccaac accaggctac
     7801 ggtcgaagta aggcaccagc acggtctggg tgacgcccac gtagcgcacc gcgagcacgt
     7861 ccgccaggac caccgcgagg gtcagcaata ggaacttgta gcgggcgctc aagccctcgg
     7921 ggcgcttttt ggagaccttc tgccagtcgt cgtagagccc gatgccggcc accacggcga
     7981 cgagcaagag cgtcgtcaac gtggcaacgt tgggccgcgc caccaccagg agcgccccaa
     8041 ggaggcccgt aagcatcacg atcccaccca tcgtcggggt gccggccttg atcagatgcg
     8101 tctgggggcc ctcctcgcgg acaaactgcc caaacttccg ggactgcaag aattcgatgt
     8161 acttcgagcc gagcaacacc gttacgagaa aagctaccgc agccgcgagt acgacgctaa
     8221 acaagagcta gactctccct caacttacgg gtcagggtat cgagcccaac cccgcgggaa
     8281 cccttgacga cgaggtagtc tctggcccgc agctcggtgc gcagggcatc ggcggcgctc
     8341 tctgcgtctg ggtagaagat tgtcctgccg gggaaggtct ctgcatacca gcgggcctcg
     8401 tcaccgacgc agacgagcag atctacgccg acttgggctg ccagttcacc ggcctcccta
     8461 tggtaagcgc gggcgcaggc ccccagctcg aacataccgc ctagcacgac gacgagacgt
     8521 ctacgttgtc tgcgtgcctg atccgcaccg tatgcgagta cggccgcgac ggcggcgggc
     8581 gatgcgttgt aggagtcatc gtagacgatg atgtcttcct tgagggagta taggtcgccc
     8641 cgcagtcccg ttcgccgcag gcgggagaga ccacgggcgg cctgctccag gtcgaggccg
     8701 agggcgagcg cgccgccgag ggcggccaaa agcggctcca ccaggtgcgt cccgaagacc
     8761 ggcgaacgaa cctcggccga ctcgccgcct atgctggcca caaagcggag gccttcttcc
     8821 gtaccttcga tctctgaggc gacgagatcc gctgtgccgg cccgggcgaa ggtgatacgc
     8881 tccagacttc ggcccgaccc tgtcgccgcc tcgggcgcgc ccgccggggc gatgagcgtg
     8941 ccggattcgg gtaaggagag ggccagttcg cctttcgcgg ctgcgaggtc ttcgagcgta
     9001 ccgaaggagt ccagatgaac cggcgagatg gccgtcagga taccgatgtc gggtggggcg
     9061 atggcgcaca ggtactgtat gtcccctgca tgggttgctc ccatctcgag gacgaggacc
     9121 tctgtacgag ggtcggaggc caggatggtg aggggaaggc cgatctcgtt gttcaggttt
     9181 ccctccgtcg ccgagacacg cctcccggca tgcctgagaa tggtcgcgag ggcgtccttg
     9241 gtcgtcgtct tgcccacggt gcccgtaatc ccgacaacgg tcggcgaagg cgcgtccccg
     9301 cgcacaagcg tccatcgcgc gagcctctgc agcgcctcca gaggatcgtc cacgacgagg
     9361 gtcggcacgt cgaggcggcg ggtcgccacc gtagccaccg cgccgcgcag gtgcgcctcg
     9421 ggggcgaaat ccgcgccgtc ggtcctgccg cgcagggcga agaacagatc cccttcgcgc
     9481 accttgcggg aatcggtcgc agccccacct acgagggtgt ttacatgcgt ggtccggagc
     9541 tccgcgtcca tagcccgcgc ggcctctgaa agagtagccg gtctcaccag aggggctcct
     9601 ctgccacaaa gcccctacgg tctgggggca cgttgaagta gcccagcgta aagctcatca
     9661 cctggcggaa ggccggtacc gcgaccagct ctccccagta ggaagtctgc ggatcgtcga
     9721 cggcaaccag catcacgtac tcggggtcgg ctgccggggc gaaccccaca aaagaggcca
     9781 cgtactggtc accgtaggtt cctgttttcg ggtccactat ctgagaggtt cctgtcttgc
     9841 cggccacggt gtaaccggag atctgggcga agtggcccga cccgtcgtcg acgacgccct
     9901 gcaacattcc gcgtactata gccgaggtct tcttgcttat cacccttggg ccctgtccct
     9961 cgggggactg ctgctgggtg acgtgcggcg ttacccttcg cccaccgttg accagcgtgg
    10021 cgtagcccga gaccagttgc agaggcgtga cggtaaagcc ctgtccgaat gggatgttgc
    10081 ctatcgagac cccgctccag tccttgtagg cagggacgat ccccaggtcc tcaccccaca
    10141 aatccacgcc ggttggtttg ccgaagccga aggcgcgcat gtaatcgtag agctgtttac
    10201 cgccgagttc tttggcgacc ttcgtcaccc caacgttgct ggagtgctcg agcacttctt
    10261 ttggcgtcat gacctcggtc tcatgcggct gggaatcgtg gatgacgtgg tcggccaccg
    10321 tcatgtgatc cgggaccacg aactcgctgg acggcgtgac cgcgccttct tcgagcgccg
    10381 acgcgacggt gaaagccttg aaggtcgagc ctggctcgta aggatcggta aggacccgat
    10441 cccgctggta ctctggggga acgtcgccga actcgttgtt gtcgtaggcc ggggtgttgg
    10501 ccagcgccag gatcgacccg tcatcgaccc gcattaccac gccgacggcg ctcctggcct
    10561 tgctctcggc gacggccttg gccagggctt tttgcaacac ctgctgcacc gcggagtcca
    10621 ccgtcaggtc tacgtccccg ccgcgcttca aggtgtcgtc gtaactggcc tcgacaccgc
    10681 cgtagggctc gccgtaatct ccctggtgcc ccaggagctg gctggcgagg gatccgtccg
    10741 gatagaccct catggtatcg ggggtcgtcg tgatgccctc tatacccatg gcctgcaccc
    10801 tggccgctgt cttcggctcg acgcccgtgg cgacgacgct gtatccactt agctggccgt
    10861 ctacaccacg cctcgtgaga gaggtcctta tctgcttctc ggtcgtgtcc gtctcttcgc
    10921 cgagctcggc gtgcagctcc ctggccgtcg cccccgggtc ttcgatctgg taaggggtcg
    10981 ctatcacacg ggcgacctcg aggctggtcg cgagctccct cccgtcggca ctaactatgc
    11041 tgccgcgggt cggcgcgacg acgggggtcg ttccaccggc ctgctcgtcg gcgaaggccc
    11101 tgtaatcctc gtcctctgtc aggcttatgt gcacggccct cccaccgagg agaagccccg
    11161 tcaccacgaa caccgccgcc accatccaca gcctcctgcg ccccacagta ggcctggctt
    11221 cgggaccggt cgaagaggcg cgacgcgccc cggtggcacc gcgcttcgcg ccgccggacc
    11281 gcctctggcc gcggagcggt atgactttac caccggagga cttgcgccct ttgtttgtgg
    11341 tgcgaggggg ggctttctta ccacggcgaa tccttgcttt gcgaggccct ggccggtcaa
    11401 cgctaccgcg gtcttccccc ttcctgtggt accccatcgt tctccccatc ctccgctcca
    11461 tagactctca aagtagtgct cttcggctcg tgcataccga gcttctccgc cgcgagggcc
    11521 cggatcctgt ccgcccccga gagttcggcg actctgacct cgagtctctc tcctacttct
    11581 ctggccctat cgagcctgac gtcgagctcg gctgccctga cgtcgagccc cgcggcgacc
    11641 gtgttcaggt agacgctccc cagcatcaac aacacgggca caacgaccag catcgcaaac
    11701 cggcgacgcc ggcccgcggc tttccgcctc acgctgcgat cccgcgggcc gtccttggcg
    11761 cgtttctgga cgggccacgc cggttcggtg gggagcgtag ccttgtacct gcgctgggag
    11821 gcggccatca gctgtcatcc accgggggct cggccgtcct gatcgccgcc cgcaacctgg
    11881 ccgaggcgct gcgcgggttc tcctcgatct ctcgccggga aggggtcagg acctcgcccc
    11941 tgcggaaaat gggctttgcg ccacaaacgc acacaggcaa ctcgggcggg cagacgcacc
    12001 gtccctcccg tcccgagatg aagcgcttta cgaggcgatc ctcccccgag tggaacgtga
    12061 tgaccacgag cctcccgccc ggtacgagaa gccgttctga ggcctccagg gcccgaccga
    12121 ggctgcccaa ctcgtcgttc accctgaccc gcacggcctg gaagatcctc ttcgccgggt
    12181 tgccgcccct ctgcttccag cccacggccg cccgcactgc ctcgtagagg tccgtcgtaa
    12241 ccgcgagcgg tcgcctccga acgacctccc gcgccacacg tcccgcctcg gcgcgcggca
    12301 cgtcgccgta ctccgagagc accctcgaca gctccccgac atcgacggtg ttcaggaagt
    12361 ccgcggccgt catacccgac cgcgggtcca tccgcatgtc gagcggtccc tcgcccacgt
    12421 acgagaaccc gcgtgccggc tcgtcgacct ggaagctgga gaggccgagg tcgaacagga
    12481 tcgcgtcggc gcctctaccc tcctcgacca tcgcccacaa cgccacgtcg taagcgccag
    12541 agacgaactc gaagcgcggg tcctcaagca gatccgatgc cctccccgcc gcctcggggt
    12601 cgcggtcgat gccgaggagg cgaccatccg gaccgaggcg ggcgagtatg agcctggagt
    12661 ggccgccacc gccgaacgtg gcgtctacga cagtgtcttc tggcgccagc ccaagggcgg
    12721 ccaccgctct ctcgagcata acgggacggt gtccggtagc catcacccgc cgagatccct
    12781 tccggcgaac tcctcgacga tctcggagaa cgtgtcgtcg gcacccgcga cgtagcggtc
    12841 ccactcctcc gtgtcccaga tctccagtcg gtcgtccacg cccgtgatgg tcacctcacg
    12901 ccgtagtccc gcgtagctgg cgagcttggt cgggatcaaa acccgcccct gcctgtccag
    12961 cgtggcctcg aaagccatgg agaagaacat ccgggagagc tggcgccccc tggccgagag
    13021 gtcagcgttg gcctttatgt tggccttgaa cgtcgcccac tcctcaggcg ggaaggcgaa
    13081 cagacacctg tccaccccct tggtcaccac gatcccaccc tcgaaatagg agcggagtcg
    13141 cgaagggagg gtcatgcgcc ctttctcatc tagcgtgtgc tcgtactcgc ctaaaagggc
    13201 cagggccgtg tcctcccact acgctccact ttgacccaca acagtgaaat tctggcatat
    13261 gcaccgaaaa tgcgcaatct ggaaggccgg aaatggccct ctacggccgg gtttcgacgg
    13321 taaacacggt tttacccctc taccccaggg tataggaccc ctatttcgag gtgatgattt
    13381 tccgggttga cgaaggattc tctacgggtg aaggctgtaa cgccggtgta acatatcggg
    13441 cgcaagagag attgaccact acatattggg tgtatggaaa taagttgggg atgtgggagt
    13501 cggtatttgg gctatgcggg tgggcccacc aggactcgaa cctgggaccg tccgattatg
    13561 agtcggatgc tctaaccaac tgagctatgg gcccttggcg ggagggcggt gccgcttagc
    13621 tcccccggct ggactcgaac cagcaaccct tcggttaaca gccgaatgct ctgccaattg
    13681 agctacaggg gaatgttaca agagtggatt atacggttcg gggcctgctg cttcaagctc
    13741 cggcggagac ggatcgcagc gcctgtttga gatgttgtat ctccgagtgc agaacgtcct
    13801 ttctatcgta atcaggagtc tcccgtttgc tgtgttcgcg gctcaggatg gcgagccgca
    13861 gccaggcttc gcgcacggag gtttcggaag ggtagagtct ctccccctgg gaggcgaggg
    13921 ccccgatctg gtccatcatc ggccgtactc tcggatccga gagcacggcg tcgaggtctc
    13981 tgccggcatg atccctgagc acggtgaaga tctttgcgtg ggtctcatcg cgaaagtcct
    14041 cgccttgcag cgtcaagggc tcggggagac ccggaatctc tatgccctct tgcaggaagg
    14101 tggctgtgag gtcgggcctg gcgaggatga gggtgagtac ggcagcgccg gcttccattt
    14161 gcgggtcagt cgggccgcga tcccgtgagg aagagccggc cggtgcggta ggtggctgcg
    14221 ccgcctgcag gacgtccggg cccacaccga gggattctgt ggccaaccgg acggcctcac
    14281 ggtagaggac gggatccttt atctcctgga tcatgttcct cacctcggga acggccctgg
    14341 agcgttccgt ggcatcagct cccctggtac gggcgactat gcgcctgatg ccgtactcga
    14401 ggacgggcac ggaatcgttc aacttccgtg caaattcttc gggcgaatgc tccaggagcc
    14461 aatccgccgg gtcttcaggg agacgcagga cccggagatc cagcttcaag tctgccgccg
    14521 tggccgccgc ccgttctatc gccctctccc ccgccgcgtc ggggtcgaag aggacgtaga
    14581 tcctatctgc ataacggctg agcatacgca gatgaccggg agtggtcgca gtacccaggg
    14641 ttgccacggc attctcgatg cccgactggt agagcatcag cacgtccgtg tagccctcga
    14701 cgacgagcgc tgatctttct ctacgaatag cctcagagac ctgggggaac ccgtagagca
    14761 gatcccgctt gttgaagatc tccgtttcgg gagagttgag gtacttgggc tgggcatcac
    14821 cgagcgtcct cgccccgaag cccacgatcc tcccccgccg gtccgaaata gggaaggtga
    14881 ccctaccggc gaaacgctcc ccaccacgcg tcgacaacag cccggccgcg tcgagaacgc
    14941 tgcggtcgag atccaacctc ccagccgctg ccacaaaccc cgaaaccccc cgcggcggtg
    15001 cataccccaa acgaaattct tctatagtag aactatctat acggcggtct ttgaggtagc
    15061 ggcgggcccg ttgtgcttcc ggggttttcg acttaaggag gtacttgtga taatagacgg
    15121 tggcggcggc gagggccttg tggatagcac gcctgcggag attgcgttcc cttgccgcgt
    15181 ccgggtctcc cccgccctcg aactgcaggt cgacgccgga gcgatccgcg aggtaggaga
    15241 ccgcttcggc gaagtctaga gatttgaggt ccatggcgag tttgatggcg tccccgccgc
    15301 gttggcagcc atgacagtag tagaggccct gatccgggga gaggctgaag gaaggggtct
    15361 tctcctggtg gtcggggtag ggacagaggc ctgtgaagcg ggttccctga cgcttgaggg
    15421 ccgtgaactc ggaggcgact tcgactatat cggccgtctc cttgacctgc tctatgctgc
    15481 gttgtgagat cctcaaaccc ggtctccctc ttaccggcgt acagatttcc gcgttggcca
    15541 gcagtaatgg tactctaccc ggcatgaata ttcgcagcga atctacacgg tgccaagtgc
    15601 caggttggct agactccgtg attgcgcgat gtaccgtctc ggcgatggct gtggtggcct
    15661 cgtcgtggcg tccgctggag aagagcgtgg gcagccggat ctccgatgtt cgccatgaag
    15721 acgggattct tggtgggcct ctggggttgc gctacgtccc gaggctgtta tagttccggc
    15781 tgtgacgaag ggaaccgcca tcagtggtgg gatagagacc aggaaccagc gtttgttgat
    15841 aaggtccggt gagaagaaga gcggcacaag ctccgcggta gactaaggca gggaatcgaa
    15901 tgaatcggtt gtacatcttg ctaacggtga tggtagtgat ggtcggagcg ctcttcaccg
    15961 ggcaccaagc cctggcggag gtcctcgccg gcaccgaggg caatgatacg atcgtgggga
    16021 ctaacaggga tgatcgcctc gagggtctcg gcggcgacga caccatcaaa ggccgccgtg
    16081 gcgacgacga gatcttcccc ggtgaaggcg acgacgacgt attcgccggc gccggcgacg
    16141 accgtatctt cgcccgcgac accgccggca gggattacat agattgcgga tccgggttcg
    16201 acgtagtcga gaccatacac cgcgacgacc ggaccttgag aaactgcgag agagcccccg
    16261 gccccagccg ggatgtcggc ggaactacgg gcacgacaga cccgagaacc accggcacca
    16321 ccacaggtac aaccaccggc accaccacgg gtaccactac gggaactaca acgggtacta
    16381 caggtaccac gggcaccacc actggtagga caaggagtac gaccacggga acaaccggca
    16441 caaccacggg aacgacgggc atcacgagta ggagaaccac gagtaccacc ggtaccacca
    16501 cgggtaccag ggataccacg ggcaccacag gcgcttcgac agggacgacc ggcggcgata
    16561 ctaccggagc taccactcga gataccaccg gggcgaacac tacaggagca gcgaccacgg
    16621 gcgataggaa ctcgccaagg gacgacgtga ttcgcgacac gatcccccag ggcggggaac
    16681 tacccaacac cggcggccct tccggtctcg tcccggcggt cgcgctgctg gcgttgctca
    16741 tcaacggggc ggtcataggg ctgttgttcg tgcgccggcg ttagccctct ccgtcggagg
    16801 caaagaaggc ggcgaatggc cgggcctcag cgtgcccgca gagagcctcg agacccttca
    16861 ctcccatcgg actcgctatt acgcaaagat ctcaccccgg ggcagaaaga gacgcctgta
    16921 cgtcgccaag gcgtatctgt cggtcatccc cgccacgaag tctattgtct cggtaagggg
    16981 gtcgggatcg ctggctaccc gctcctctgg acgctccagg tagtaccaga agaggtccgt
    17041 gacgacgcgg gccgctttct ggttctcccg cgaacccagc tcgttgtcgt acacgtgccg
    17101 gaaaagccac gtcctgagct ccatgagggc ctcgtataca ggatccgaaa gggagacctc
    17161 acctttcatc ttagagttag agatcatgtc cctcaccagg gtgtcgatcc tggcgctcat
    17221 ctgctctccg agaacggaga tagggttcgg tgggaggtca tcgctagaga tgatccccgc
    17281 ccgcagcgcg tcgtcgacgt cgtggttgac gtacgcgatc ctatcggcga cgcgtacgat
    17341 ctcaccttct ctggtgcgtg gatagcccgt tcctgtatgg ttgagtatcc cgtctctgac
    17401 ctcgaaggtg aggttcagac cgtcgccttg attctcgaga tggtcgatga cgcgcaggga
    17461 gtgttcgttg tggcggaagg tgcgtccgtg ggctgtgagg gcgcgggaga gggcttcttc
    17521 tccggtgtgt ccgaagggag tgtggccgag atcgtggccg agggagaccg cctccgtgag
    17581 gtcttcgttg aggcctaggg cccgggctat ggtacgggat atctgcatca tctccagggt
    17641 gtgagtgagg cgtgtcctga agtggtcgcc atcgggggcg atgaagacct gggtcttgtg
    17701 catgagtctg cggaacgcct tggcgtggat aatacggtcc ctgtcccgct ggaagtcatt
    17761 gcggacaccg tcgggagact cgggcctggg gcggcctgcg ctatgttcga cggcccaggt
    17821 ggatggatcg tggcgaaggc cgacaccggg tccttgggat ctcacgcgcg gcgtccggcg
    17881 ccagcgaggc gcgggccggc cagggtattc cctggcgcgt gggcgaggac gtgggagcgg
    17941 accaccctga ggacgctccc ttcgggtcct gtttgcgcgg gcgcttgacg gatgggactt
    18001 tcgaccagga ttctcaggct ctccagtgtg cctggggcga gcgggaaagc gtcgcctgtc
    18061 gcggggcggc actcctggca cagaacgccc ccgtggtcgg gggcgaagtg gagcggttcc
    18121 gaagccgcgt cgaggttcgt tgcgcaggcc agacacacgt cgagttgcgg cgcgtagccg
    18181 gagaggatgg agagttttat gccgagggcc gcctcgatgt tctcgaaacc agaatcgctc
    18241 gtctccagcg tgtgcagggc gttgaagagc aggttgaaga ctctacggtc cgcctcgtcg
    18301 cctcccgaga gggcgcgcac gctcccgacc attcccgcgg cggtctcgaa gcgggcgaga
    18361 tcctccctga taccgtggaa gctgcgcagc gtctccgcct gggtcacggt atcgagcgtc
    18421 ctgccgtggt aggcgacgaa ttcgacgcag gagagcggtt cgaggcgggc tccgaaccga
    18481 gacttcgtac gcctgatgcc cttcgcgatt gccgagacca gccctgcttc cgtggtgtag
    18541 aggtccagga tccggtccgc ctcagagtag cggatcgaac gtagcacgat ccccttactc
    18601 ttgtagaccg ccaccttcgg cctccttgat cacggcgacg ctccggctca tgccgaggcg
    18661 cgaagcccct gcgttgagta tgtcgagggc gtccccgagg gagctgatgc cgcccgacgc
    18721 ctttaccgca aggccctcag gggcttcttc cctgagcagg gccacgtctt cgggcgttgc
    18781 gcctgcgggg gcgaagcccg tcgaagtctt cacgaaatcc gcgccgctcg cggccacgat
    18841 ctgggtcgcc agccgcttca tcttgtccga taggtaagcg gtctcgacga tgaccttgac
    18901 taccacgtcg ttcaagccat tgttcatggc taccgcgctc acctcctggg ttaggcttgc
    18961 gagctcgtcg gcgacgtagc cgaaatctcc ggagaggaac ttcgagatgt tcatcaccac
    19021 gtcgagctcc gaagcgcccc tggtgaccgc ctcgcggacg gccgcaatct tcaccgcggt
    19081 ggactcgtac ccgaaaggga aggagatcac cgtcgccacc tttacgtcgg cgccacgcaa
    19141 ctccctggcg gcgagaggga cgtaggaggg cgggatgcac accgcggcga agtgatagtg
    19201 ggcggcctcc ttgcagaggc ttacgatctc ctgctcggtg gcccctggct tcaagagagt
    19261 atggtccacc gttttcgcga actcacgcac cctcatcgcc aaagcgttac gctcctctct
    19321 cgacagatca gggtgattat aaacccaacc gctccagaaa ctgcctgtcg cttctccatc
    19381 cctggctaac cttaactttc aggtcgaggt agatacgggt gccgaggagg cgctcgacgt
    19441 cgcggcgggc ttcggtaccg atcctcttta tcgtcttgcc gcctttgccg agcacgatca
    19501 tacgttgcgt ggcgcgttcg acgtggatga cggcgtagac gacggtcacg ttctctttgc
    19561 gctctacgtc ttcgatctcg actgcgacgg catgcggcac ctcttccctc agcacgttca
    19621 gcgccttctc ccgaacgtac tcggcgagga ccagagactc ggggtagtcc gtcaccgtgc
    19681 cctcggggaa atagcgggga ccatgcggca ggagaccgac aacggaatcc atcagaggat
    19741 cgacgttggt gccgttggcg gcgctgatct ggaacacctc gttccaatcc ccgagccgcg
    19801 agaccgcttg tactatcggc agggcgctct cacgggtgcg aagcaggtcg gtcttgttga
    19861 tggtgacgat ggccggggtg cccgagccgg cgacgaggcg ggcgatgtag cggtcgccgc
    19921 ttcctagtcc ttccgaggcc tgcggcgcgt cgaagagcaa gaggatgaca tccgactcag
    19981 agaggctgtc cacgacctgt tc
//
LOCUS       DATMVF010000144        10760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_296388, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000144 DATMVF010000000
VERSION     DATMVF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296388"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4359)
                     /gene="gltB"
                     /locus_tag="VN178_07330"
     CDS             complement(<1..4359)
                     /gene="gltB"
                     /locus_tag="VN178_07330"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWS80821.1"
                     /translation="MLTPGAGLYDPMYEHDACGLGFVARLDGRRTRETVEEGLEVLHN
                     LEHRGTTGSDPGTGDGAGILTQIPDGFFRRECEELGIELPAAGHYGLGMLFESGQVGA
                     LDCGGKLAEICAEEGQDLLGFREVPIVPEAVGKVARSVMPRIRQFFIGRRGGDEAAFE
                     RKLYVIRRLLHEAVEDSHGCYVVSLSAKTIIYKGLLKGQQLPRFYPDLGDAAFASAIA
                     LVHERFSTNTLGSWELAHPYRYIAHNGEVNTIRGNINWMRARESRLSSELFGDDLEKL
                     SPVILPGQSDSASFDNALELLHLAGRSLPHAVAMMIPEAWENDGLMDPDRRAFYQYHS
                     ALMEPWDGPAAVAFTDGRIIGATLDRNGLRPARYSVTKDGRVIMASEDGALRVPAEEV
                     VERWRLQPGKMLVVDTERHELLHDEDVKRPLLGRQPYRAWLEEGEIHLNELPEPAADP
                     RPEPTTLLERQRAFGYTIEDQRILLAPMAQDGKEPDGSMGTDTPLAVLSERPQLLFSY
                     FKQHFAQVTNPPIDPLREELVMSLKMSLGPEQNLFDETKKHCRRILIDQPILTAAELE
                     KIRALGDERASSATLSTLFPVSAGEEGMEEALTRLCEKAESAVKAGSAVLVLSDRGVN
                     ETQAPIPSLLATASVHHHLVRAGIRTATTLVVETAEAREVHHFALLVGYGATAVNPYL
                     AFETIEDLAGSHLMEGVGPEEAAKNYVKAVGKGLLKVISKMGISTLFSYCGAQIFEAV
                     GLNQRLIDRCFTGTASRVGGVGMDTLARETLERHRSAFSAVWDNAGELEVGGEYQLRA
                     QGQYHQWNQTSIPPLQRAVKTGSFETFKEFTHHFDEHSARFATLRGLFGFEESPVPIE
                     EVEPASEIVKRFTTGAMSLGSLSKEAHETLAIAMNRIGGKSNTGEGGEDPRRFTPDEN
                     GDSRRSAIKQVASGRFGVTTEYLVNADQLQIKMAQGSKPGEGGQLPGGKISEYIAKIR
                     YSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPRAAVSVKLVAEVGVGTVAAGVAK
                     AKADHITISGHDGGTGASPLSSIKHAGLPWELGIAEAQQVLVENGLRGRVILQTDGQL
                     KTGRDVTVAALLGAEDFAFSTAPLVATGCIMMRVCHLNTCPVGIATQDPELRKRFTGT
                     PEHVINYFFFIAEEVREYMAKMGFRTFEEMIGRADRLKMRQAIEHWKARGVDLRALLH
                     RVEAPEGGAIRHSESQDHGLERSLDNELIEKCLPALENGKRISFEQPITNTNRTVGGM
                     LSGEVARRYGNEGLPDGTVRIDFHGVAGQSFGAWLASGLTFTLEGTTNDYVGKGLSGG
                     RLAVFPSRLAAYKPTRNVVVGNVALYGATAGEAYFRGFAGERFAVRNSGAHAVVEGVG
                     DHACEYMTGGVVVVLGETGRNFAAGMSGGVAFVLDEAERFETLCNLDMVGLEAVESEE
                     DIELLHGMVERHLEWTG"
     gene            complement(4601..4927)
                     /locus_tag="VN178_07335"
     CDS             complement(4601..4927)
                     /locus_tag="VN178_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80822.1"
                     /translation="MANRFFKQTSAEIISDLLSDARVKRVREYVQGEEFKADRERVRR
                     DAAERLKNVRDRYRAKRRDPETAARERELTLRLAEVEAEAAELRIQLSVLLEEEEELR
                     RSLDEL"
     gene            complement(4979..5533)
                     /locus_tag="VN178_07340"
     CDS             complement(4979..5533)
                     /locus_tag="VN178_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HWS80823.1"
                     /translation="MEDDGRMSEDEGYAYQAGRDRGESAGDPDLQLDVPALSVEEIDL
                     EVENLQLRLSIQAELADLVKIKVGLDAELGELKLGIKGVQAQAQLKARLDNVRAIFSE
                     VLASLEHNPRFFREPVSMEALDEIGDVSGQSADEEAEHREEPEATEAATRRAKELGVD
                     LSGLEGTGAGGRVVVKDVTRAAEG"
     gene            5697..5918
                     /locus_tag="VN178_07345"
     CDS             5697..5918
                     /locus_tag="VN178_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80824.1"
                     /translation="MTQRDQQQGQQQEGQQPSPLQLLGRALTDIRKVQNLLELQYPDQ
                     GDAIKMQREAGDLVWNEIQRMQQQQQSQQ"
     gene            complement(5942..6235)
                     /locus_tag="VN178_07350"
     CDS             complement(5942..6235)
                     /locus_tag="VN178_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80825.1"
                     /translation="MYRARNAARLASFEDRRMWRLLLARLHPDAGGDHDLFAFACAVR
                     DEMSEGEHPVEDAAGEDEAGNTESVVPFLRTWHEAMGQWSSSNRDALNNLRTR"
     gene            complement(6427..6864)
                     /locus_tag="VN178_07355"
     CDS             complement(6427..6864)
                     /locus_tag="VN178_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80826.1"
                     /translation="MSEVQYQQRGDQQAQDAGQVTDQAGQVTDQAQDAAGQVAGQAQD
                     VAGQATDQAGQLVGQTQNAVGGLIGGGQGDGRSGEQDMDDYSYSDSDATTVENDKTPK
                     GILGQLLNLDILPPPQSDTTSLESGDSAEYDGPEEAQQEEAQG"
     gene            complement(6861..7085)
                     /locus_tag="VN178_07360"
     CDS             complement(6861..7085)
                     /locus_tag="VN178_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80827.1"
                     /translation="MADKRVRGSMLARQHAADLRANDVIRRIERGGMDPRPLPSLRED
                     SMASMESMPSMESAETVSGVTGKSNERRRQ"
     gene            complement(7210..7803)
                     /locus_tag="VN178_07365"
     CDS             complement(7210..7803)
                     /locus_tag="VN178_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80828.1"
                     /translation="MSTEHRRRVPEDLMGTILGESRGFPDEEQIISESGEATVSGEDD
                     SELVGVAFNLSKQLGAELDRIRHELEEETHPSKSEIAEVALRIAVEDYRSRGRDSDLV
                     KRIEERHEARTGDAPRKVVRSVDQTGLIIETTYDESGGVLDEDVVGRVADLPVVDEYL
                     DEQGRLVSQVQDDLGNTFEQVQDNNLNTLEVRLLNGL"
     gene            complement(7805..8533)
                     /locus_tag="VN178_07370"
     CDS             complement(7805..8533)
                     /locus_tag="VN178_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWS80829.1"
                     /translation="MTNAKGGVAKTTSCVNLGAGLALLGYRTLLVDCDTQGQVGQSLG
                     VQAGEGLGELLQGEAGLADVQIEARENLYVIAGGGSLSGAKREISRRDMRSELVLSEA
                     LYDLDGYDFVLLDTAPSWDVLNVNCLFYVEELLIPVSMEALALQGIGDLVSRVEQVQH
                     YREERVLQKGIIPTFYDGRVNKSAEILEQLERYFPGAVWPIVRYNVKLSEAPGFGQHI
                     FEYAKRSNGAADYARLAKRLAKGA"
     gene            complement(8672..8974)
                     /locus_tag="VN178_07375"
     CDS             complement(8672..8974)
                     /locus_tag="VN178_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80830.1"
                     /translation="MASSAGGDDRERELKERLIKAARMYAMCEKAGVPEPMDVSGLAV
                     AAFEDLPLREALVFVRTNEQNVRDLAWAFANSGSAEEFEQRLGEIKTLPEPGGPRR"
     gene            complement(9005..9418)
                     /locus_tag="VN178_07380"
     CDS             complement(9005..9418)
                     /locus_tag="VN178_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80831.1"
                     /translation="MPRRRRRNRRRGPRGTSVRVTTTRNGKTTSYRTGGISITGVGPT
                     GRVARSRNLTIRATLWDYGGGLSKNGIRLYLDGSEKSRFRYDEASGSLTYYVGRALSP
                     GTHEVRIEAESESSDKHGRSSGSRTKRWTFTVARR"
     gene            complement(9467..9850)
                     /locus_tag="VN178_07385"
     CDS             complement(9467..9850)
                     /locus_tag="VN178_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80832.1"
                     /translation="MGRSRRRRTSHRSNPRTRSGARSRPGRGGPRGGGVTITSVSPVG
                     NISGYRPTVQATVKNGQSTLTKDDINVYLDGQEVRDFQYRRTTGSLSFTPRKLASGAH
                     TVEITAGTSGSRNARKRKNWSFVVP"
     gene            complement(9994..10251)
                     /locus_tag="VN178_07390"
     CDS             complement(9994..10251)
                     /locus_tag="VN178_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWS80833.1"
                     /translation="MDDKAAGKAKEAWGALTGDEAKKEEGRAQQRKAEAQEEATQAAR
                     TNQAKKDARQAEREREREKIKEKGLLGGVTDSLTGRDRDRS"
     gene            complement(10295..>10760)
                     /locus_tag="VN178_07395"
     CDS             complement(10295..>10760)
                     /locus_tag="VN178_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HWS80834.1"
                     /translation="GLTGGQQQGGQQQGGPLGGVTDQVGQVAQGVQDTAGQAAGQAQQ
                     AAGQATDQAGQVAGQAQDAAGGAAQQAQDTAGQAAGQAQQVAGQAQDTAGQAAQQGQQ
                     AAQQAAGGQQQPEATHAAQQKAQELGVDLSQVQGSGAQGRITVKDVISASNQ"
BASE COUNT         1588 a         3769 c         3216 g         2187 t
ORIGIN      
        1 ccccgtccac tccaggtgac gctcgaccat cccgtgcagg agttcgatat cttcctcgct
       61 ctccacggct tcgagaccga ccatgtccag attgcacagg gtctcgaagc gctccgcctc
      121 gtcgagcacg aatgctacgc cgccgctcat gccggctgcg aaattgcgcc ccgtctcgcc
      181 tagcaccacg acgaccccgc cggtcatgta ctcgcaggca tggtcgccca cgccctcgac
      241 cacggcgtgg gcgcccgaat tcctgacggc gaagcgctcg cccgcgaagc ctctgaagta
      301 cgcctctcct gcggtcgccc cgtagagcgc cacgttgccg acgacgacgt tcctggtcgg
      361 cttgtaggcg gcgagcctgg aggggaagac ggcgagccta cctccggaga gtcccttacc
      421 gacgtagtcg ttggtcgtgc cttccagggt gaaggtaagg ccgctggcga gccacgcacc
      481 gaaggactgg cccgcgacgc cgtggaagtc tatgcggacc gtgccgtcgg gcaggccctc
      541 gttgccgtaa cgcctggcta cctcccccga gagcatcccg ccgaccgtgc ggttggtgtt
      601 cgttatgggt tgctcgaagc tgatcctctt gccgttctcg agggccggca ggcacttctc
      661 gatcaactcg ttgtccagcg acctctcgag cccgtgatcc tggctctccg agtggcggat
      721 cgcccctcct tccggtgcct ctacccggtg gagcaacgcc ctgagatcca cgccgcgggc
      781 cttccagtgc tcgatggcct ggcgcatctt gagtctgtcg gcgcgcccga tcatctcctc
      841 gaaggtcctg aagcccatct tcgccatata ctcccgcacc tcttcggcga tgaagaagaa
      901 gtagttgata acgtgctcgg gcgtaccggt aaagcgcttt ctgagttcgg gatcctgggt
      961 cgcgataccc accgggcacg tgttcaggtg gcatacgcgc atcatgatgc aacccgtggc
     1021 cacgagcggc gccgtcgaga aggcgaaatc ttcggccccg aggagcgccg ccacggtgac
     1081 gtcgcggccc gtcttgagct ggccgtcggt ctgcaggatc acacgccccc gtagcccgtt
     1141 ctcgactagc acctgctggg cctcggcgat ccccagctcc cacggcagcc ccgcgtgctt
     1201 tatggaagag agcggggagg cccccgtccc gccatcgtgg cccgagatgg ttatgtggtc
     1261 ggccttcgcc ttggccacgc ccgccgcgac agtccctacc ccgacctcgg cgacgagctt
     1321 cacgcttact gcggcccgtg ggttggcatt cttcaggtcg tggattagct gcgctaggtc
     1381 ctctatagag tagatgtcgt ggtggggggg cggggagatc agacccaccc caggcgtgga
     1441 gtaccgtatc ttcgctatgt actcgctgat cttgccccca ggcaactggc cgccctcgcc
     1501 cggcttggac ccttgcgcca tctttatctg caactggtcg gcgttgacca ggtactccgt
     1561 cgtgacgccg aagcggccgc tcgcaacctg ctttatggcg ctgcggcggc tatctccgtt
     1621 ctcgtccggc gtaaagcgcc tggggtcctc gccgccctca ccggtgttgg atttgccgcc
     1681 tatgcggttc atcgcgatgg ccagggtctc gtgcgcctcc ttggagaggg agcccaaaga
     1741 catcgcgccc gtggtgaagc gcttgacgat ctcgctcgca ggctcgactt cctctatcgg
     1801 gacggggctc tcctcgaacc cgaagaggcc gcgcagtgta gcgaatcgcg cgctgtgctc
     1861 gtcgaagtga tgggtgaact ccttgaaggt ctcgaagctc ccggtcttta cggcgcgctg
     1921 gaggggaggg atgctcgtct gattccactg gtggtactgg ccctgcgccc tgagctggta
     1981 ctcgcccccg acctcgagct cccccgcgtt gtcccaaaca gccgagaagg cactcctgtg
     2041 acgttccagg gtctcgcgcg ccaaagtgtc catccctacg ccgccaacgc gagaggcggt
     2101 gcccgtaaag cacctgtcta tgagcctctg gttcaggcca acggcctcga agatctgggc
     2161 cccgcaatag gagaagagcg tggagatgcc catcttggag atgaccttga gcagtccctt
     2221 tcccacggcc ttgacgtagt tcttcgccgc ctcctcgggg ccgacgcctt ccatgaggtg
     2281 cgagcctgcg agatcttcta tcgtctcgaa ggcgaggtat gggttcacgg cggtcgcgcc
     2341 gtagcctacc agcagggcga aatggtggac ctcacgcgcc tcggcggtct ccacgacgag
     2401 ggtcgtggcc gtccttatgc ctgcccgcac caggtggtgg tgaacgctcg cggtggcgag
     2461 gaggctgggt atcggggcct gcgtctcgtt cacgcccctg tcgctcagga ccaggaccgc
     2521 agatcctgcc ttgaccgcgc tctcggcctt ctcgcaaagc ctggtcaggg cctcctccat
     2581 cccctcctcg ccggccgaga cggggaacag ggtggagagc gtcgcggagc tcgcccgctc
     2641 atcgcccagg gcccggatct tctcgagttc cgccgccgtg aggatcggct ggtcgatgag
     2701 tatccgcctg cagtgcttct tcgtctcgtc gaagaggttc tgctcgggcc cgaggctcat
     2761 cttgagcgac atgaccagct cctcgcgcaa gggatctatc ggagggttgg tgacctgcgc
     2821 gaagtgctgc ttgaaatacg aaaacaggag ctgcggccgc tcggagagca cggccagggg
     2881 cgtgtccgtg cccatggaac cgtcgggctc cttgccatcc tgggccatcg gggcgaggag
     2941 tatgcgctgg tcctcgatgg tgtacccgaa ggcccgctga cgttcgagaa gcgtggtcgg
     3001 ttcgggacgc gggtcagcgg cgggctcggg gagctcgttg aggtggatct caccctcttc
     3061 gagccatgct ctgtacggct gccgtcctag gagcggcctc tttacgtcct cgtcgtggag
     3121 cagctcgtgt ctctcggtgt cgacgacgag catcttgccc ggctggagcc tccagcgttc
     3181 gaccacttcc tcggcaggca ccctcaaggc cccgtcctcc gatgccatga tgaccctgcc
     3241 gtccttggtg accgagtacc gcgcaggacg caagccgttc ctgtccagcg tggcgccgat
     3301 gattcggccg tcggtgaagg cgaccgcagc cggtccgtcc cacggctcca tgagggcgct
     3361 gtggtactgg tagaaggcgc gccggtcagg gtccatcaac ccgtcgttct cccaggcctc
     3421 agggatcatc atggccaccg cgtgcggcag gctccgcccg gcaaggtgca agagctcgag
     3481 ggcgttgtcg aaggaggccg agtcgctctg gccgggcagg atgaccgggg agagcttctc
     3541 cagatcatcc ccgaagagct ccgaactcag gcggctctcg cgggcccgca tccagtttat
     3601 gttgcccctt atggtgttga cctcgccgtt gtgcgcgatg tagcggtaag ggtgcgcgag
     3661 ctcccaactg cccaaagtgt tcgtcgagaa ccgttcgtgc acgagcgcta tcgcgcttgc
     3721 gaacgccgca tccccgaggt cgggatagaa ccgcggcaac tgctgaccct tcaagagacc
     3781 tttgtagatg atcgtcttcg cggagaggct gaccacgtag cacccgtgcg aatcctcgac
     3841 ggcctcgtgc agcaggcgcc ggatcacgta gagcttccgc tcgaaagccg cctcgtctcc
     3901 gccgcggcgc cctataaaga actgcctgat gcgcggcatg acgcttcggg cgaccttacc
     3961 gaccgcctcc ggcacgatgg gcacttccct gaaaccgagg agatcctggc cctcctcagc
     4021 gcagatctcc gccagcttac ccccgcaatc gagcgccccg acctgccccg actcgaagag
     4081 catccccagc ccgtaatgtc ccgccgcggg aagctcgatg ccaagctctt cgcattccct
     4141 gcggaagaat ccgtcgggga tctgggtgag tatcccggcc ccgtcgcccg tcccgggatc
     4201 gctccccgtc gtccccctgt gctccaggtt gtgcaggacc tcgaggccct cctcgaccgt
     4261 ctcccgcgtc ctgcgcccgt cgagcctggc tacgaacccg agcccgcaag catcgtgctc
     4321 gtacataggg tcgtaaagtc cggctccagg ggtcaacaag tcctctccta tggtgtccag
     4381 ggtgcggaac gctcattttt acacgaactg ccaacagagc acaagtgggt aacaatcctg
     4441 ttgcatacta gtatctggta aggcttcagg aatgtgcaaa gaggtccaag acctctttgc
     4501 acgctatcag ctttcagcca agaacaataa gctgagcgct gaaggctgat agctgaaggc
     4561 gcgatccgca gagggtcttt gggaccctct gcggatcgct tcagagctcg tcgagggatc
     4621 tccggagctc ttcctcttct tcgagcagga cggagagttg gatgcgcagc tcggcggcct
     4681 cagcctctac ctcggcgagg cgcagggtga gctctctctc gcgggcggcg gtttcggggt
     4741 cgcgccgttt ggcgcggtag cggtcgcgga cattcttgag gcgttctgcg gcgtcgcggc
     4801 gcacgcgttc gcggtcggct ttgaactcct cgccctgcac gtactccctg acgcgtttca
     4861 cccgcgcgtc ggagaggaga tccgagatga tctccgccga ggtctgcttg aaaaacctgt
     4921 tggccacggg cttcctcctt ctactccacc agattgtata cgctaagggt ggtagaggtt
     4981 atccctccgc ggcccgcgta acgtccttga cgacgacgcg cccgccggca cctgtgcctt
     5041 cgagaccgga gaggtccacg cccagctcct tcgcccttct cgtggccgct tccgtcgctt
     5101 ccggttcttc gcgatgttcc gcttcttcgt ccgccgactg gcccgagacg tcgccgatct
     5161 catccaaggc ctccatgctc accggctcgc ggaagaagcg cgggttgtgt tctaacgatg
     5221 ccagcacctc gctgaagatg gcgcgcacgt tgtcgaggcg ggccttcagt tgggcctggg
     5281 cttgtacgcc tttgatcccg agctttagtt cgcccagctc ggcgtctagt cccactttga
     5341 tcttcaccag gtccgccagc tcagcctgaa tggacaggcg gagttggagg ttctcgacct
     5401 cgagatcgat ctcctcgacg ctgagggcgg gcacgtcgag ctgcaggtcg ggatcccccg
     5461 cgctctcccc acggtcccgc ccggcttgat aggcgtagcc ctcgtcctcg ctcatgcgcc
     5521 cgtcgtcttc caatgtcggc ccctccgatg ccatgcgccc ctctccaatg tcccgttacc
     5581 catctgtcat gcccctaccg tccggcaaat aaactcgccg gggcacggcg cctgtcttac
     5641 gccggcccat acagggtata cacgacccac agacagcatc ggttcaggag gtctcgatga
     5701 cacaaagaga tcagcagcag ggtcaacagc aagaaggtca gcagcctagc ccgttgcagt
     5761 tactcggacg cgcgttgacc gatatcagga aggtacagaa ccttttggag ttgcagtatc
     5821 cggaccaggg ggacgcgatc aagatgcagc gcgaagccgg agacctggtc tggaacgaga
     5881 tccaacgcat gcaacaacag caacagagtc agcaatagca acgtctatct ggcgcaccac
     5941 tctagcgggt ccggaggttg ttgagggcgt cgcggttgct ggaggaccac tggcccatcg
     6001 cctcgtgcca cgtgcgcagg aaaggcacca cgctttccgt gttcccggcc tcgtcttcgc
     6061 cggccgcgtc ctccacgggg tgctctccct cggacatctc gtctctcacg gcgcaggcga
     6121 acgcgaacag gtcgtgatcc ccgccagcgt ccgggtgcag gcgcgccagt agcaaccgcc
     6181 acatcctccg gtcctcgaag gatgcgagcc tcgccgcgtt tctagctctg tacaccagat
     6241 aggcctccgt ccggcgcatt ctatgcgggg gcacgaaacg ttgtaaaccg cgttatcccg
     6301 caaccgctac acctggagga acggtcgcgc gccggtccca cataaagcgg tcagctaaca
     6361 gccttcagct tttcggctga taccggctcc gagctaacgg cgccgcgcaa gggcctacgt
     6421 cttgcgctac ccttgtgcct cctcttgctg ggcttcttcc ggtccgtcgt actccgccga
     6481 gtcgccggac tccaggctgg tcgtgtcgct ctgtggggga gggaggatat ccaggttgag
     6541 gagttgcccg agaatgccct tgggggtctt gtcgttttcg accgtcgtcg cgtcgctgtc
     6601 gctgtaggag tagtcgtcca tgtcctgctc gcccgacctt ccatctccct gcccgccgcc
     6661 gatgagaccg cctacggcgt tctgggtctg gcccaccaac tgaccggcct gatccgtggc
     6721 ctgtcccgcg acgtcctgcg cctgaccggc gacttgtccg gcggcgtcct gagcctgatc
     6781 ggtcacctgg cctgcctggt cggtgacctg cccagcgtct tgcgcctgct ggtcgcctcg
     6841 ttgctggtat tgtacttcgc tcactgcctt ctcctttcgt tacttttgcc tgtcactccg
     6901 ctcaccgtct ccgcggactc catcgacggc atggattcca tagaagccat cgaatcttcc
     6961 ctgaggctcg gcaggggacg cgggtccatc ccgcccctct ctatcctcct tatcacgtcg
     7021 ttagccctca ggtcggcagc atgctgtcgc gccagcatgc tcccacgcac gcgcttgtcg
     7081 gccatgcgca tccgcccgtc gatcctgcgc tccgcctcct tctggcgccg ttcctgatcc
     7141 cgtacgctta cgacagaccc ccgtacccga tgccatgatg tctcgtcgag ggtggttcgc
     7201 gaaccgcccc tacaaaccgt tcaacaacct cacttcgagc gtgttgaggt tgttgtcctg
     7261 gacttgctcg aaggtgttgc cgaggtcgtc ctgcacctgg ctaaccaagc gtccctgctc
     7321 atccagatat tcgtctacca ccggcaggtc ggcgacgcgg cctaccacgt cctcgtctag
     7381 gaccccgccg ctttcgtcgt aagtggtctc gatgatcagg ccggtctggt ccacgctgcg
     7441 cacgaccttg cgaggggcat cgccggtcct ggcctcgtgg cgctcttcga tcctcttgac
     7501 cagatcgctg tcgcggcccc tgcttcggta gtcctcgacg gcgatgcgga gcgccacctc
     7561 ggcgatctcg gacttcgacg ggtgcgtctc ctcctcgagt tcgtgccgga tgcgatccag
     7621 ctccgcgcct agctgtttgg agaggttgaa ggccacgccc accagctcgg aatcgtcctc
     7681 ccccgagacc gtagcttcgc cggactccga gatgatctgt tcttcgtcgg ggaacccgcg
     7741 gctctcgccg aggatggtcc ccatcaggtc ctccggcacc cttcgcctgt gctccgtgct
     7801 catttcacgc ccctttcgcg agtcgcttgg ccaggcgggc gtagtcggcg gccccgttgc
     7861 tccgcttggc gtactcgaag atgtgctggc cgaaaccggg cgcctcggag agcttcacgt
     7921 tgtagcgcac gatgggccac accgcaccgg ggaaataccg ctccagctgc tccaggatct
     7981 cggcgctctt gttcacccgg ccgtcgtaga acgtcgggat tatgcccttt tgcagaaccc
     8041 gctcctccct gtagtgttgc acctgctcga cgcggctcac gaggtccccg atcccctgca
     8101 acgccagcgc ctccatggag accggtatca agagctcctc gacgtagaac aggcagttga
     8161 cgttcagcac gtcccacgaa ggcgccgtgt cgagcaacac gaagtcgtat ccatccaggt
     8221 cataaagcgc ctcgctcaag accagctccg agcgcatgtc ccggcgggag atctcgcgct
     8281 tcgccccgga gagcgacccg ccgccggcga tcacatacag gttctcgcgc gcctcgatct
     8341 gcacgtcggc gagcccggcc tcgccctgca agagctcgcc caacccctcg ccagcctgca
     8401 ccccgaggct ctggcccacc tgcccctggg tatcacagtc tacgagaagg gtcctgtacc
     8461 ccaggagggc aagccccgcg ccaaggttga cgcaagaggt ggtcttggcc accccgccct
     8521 tcgcattggt caaggctatc tttctcaacc ccaccccccg catatttgct cagcccgcaa
     8581 cggccccgat cccgcccccg tgatcgcttg tgtcccgtcc tggcggggcc ttcgcatggt
     8641 tgccgccatc cggagagccc aacccaccct tctaccggcg cggtccgccg ggttcgggga
     8701 gcgttttgat ctcgccgagc ctctgctcga actcctcggc cgacccggag ttcgcgaagg
     8761 cccaagcgag gtccctgacg ttctgctcgt tggtccgcac gaacacaagg gcctccctga
     8821 gcggcaaatc ctcgaacgcg gccaccgcga gcccgctcac gtccatgggc tcgggcaccc
     8881 cggccttctc gcacatggcg tacatccggg ccgccttgat gagccgttct ttgagctcgc
     8941 gttccctgtc gtcgccgccc gcgctagagg ccatcttgct cccggtccgg agctttccgg
     9001 cttctcaacg gcgcgccacc gtgaaggtcc acctcttcgt gcgcgaaccg ctcgaccgtc
     9061 catgtttgtc ggaggactct gattctgcct ctatcctcac ctcgtgggtg cccggggaga
     9121 gggcgcggcc gacgtaatag gtcaggctgc cgctggcctc gtcgtagcga aacctgctct
     9181 tctcggaccc gtcgaggtac agccttatgc cgttcttgga gagtccgccc ccgtagtccc
     9241 acaaggtcgc ccggatggtg aggttgcgac tccttgcgac cctgcccgtg ggaccaacac
     9301 cggttatcga gatgccgccc gtgcggtagc tcgtagtctt gccgttcctc gtcgtcgtga
     9361 ccctgaccga ggtcccacgc ggacctctcc ggcggttcct cctccgtctc ctaggcacga
     9421 gttggccccc ttagacggtc ccggacgtcc ctacttccgg cacccattac ggtacgacga
     9481 agctccagtt tttccgcttc ctcgcgttcc tgcttccgga ggtgccggcc gttatctcca
     9541 ccgtgtgggc gcccgaggcg agcttgcggg gggtgaaact caggctgcct gtggtccggc
     9601 gatactgaaa atcccttact tcctgcccgt cgaggtacac gtttatgtcg tctttggtca
     9661 gggtgctctg gccgttcttc acggtggcct gcaccgtggg cctgtagccg ctgatgtttc
     9721 ccacggggct caccgaggtt atggtgactc cgccccccct tgggccgccg cgacctggcc
     9781 tcgacctggc cccggatctc gtcctggggt tggatctgtg cgatgtcctc cgccttctag
     9841 atctgcccac gctcgctccc ttctcaacct gtcacgttcg atggcctcgt acacgtctcc
     9901 agttccggaa aggtctcgcg tcagacgcga agccgcatgc cagtgagaac tcgcacgcgg
     9961 ctccgctcgg tcaattgcga agcatcctcg cgtttaggac ctgtctcgat cacgcccggt
    10021 caaggagtcc gtcacgccac cgaggagtcc tttttccttg atcttctccc gctctcgctc
    10081 cctctctgcc tgcctggcgt ctttctttgc ctggttggtc ctcgccgcct gggtcgcctc
    10141 ttcctgggct tccgccttcc gctgctgcgc ccggccttcc tccttcttgg cctcgtcgcc
    10201 ggtcaaggcg ccccaggcct ctttggcctt gcctgcggct ttgtcgtcca cgactctact
    10261 cctttcctcc gttgggccgc cgtagccctc tcgactactg gttcgaagcg ctgatcacgt
    10321 ccttgacggt aatgcggccc tgcgccccgg agccctgtac ctgggagagg tccacgccca
    10381 gctcctgggc cttctgctgc gcggcgtgcg tggcctccgg ctgctgctga ccgccggccg
    10441 cctgctgcgc cgcctgttgg ccctgctggg cagcctgtcc ggcggtgtcc tgggcctggc
    10501 ccgccacctg ctgggcctga ccggccgcct ggcccgcggt atcctgcgcc tgctgcgccg
    10561 cgccgccagc ggcatcctgc gcctggcccg ccacctggcc ggcctggtcg gtggcctgac
    10621 cggcggcctg ctgagcctgg cccgctgcct ggcctgccgt gtcctgcacg ccctgggcga
    10681 cctgccccac ctggtcggtg accccgccga gcggtccgcc ctgctgctga ccgccctgct
    10741 gctggccgcc ggtcaggcca
//
LOCUS       DATMVF010000145         7609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_244743, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000145 DATMVF010000000
VERSION     DATMVF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244743"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VN178_07400"
     CDS             <1..1142
                     /locus_tag="VN178_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS80835.1"
                     /translation="RREAPGGNASLQRLILERLESGLTWLEHRGGALLARETGNPLTF
                     GARFDPERTVSALVDRIEAAGGEIIADSALETLVEDPAGRVTGVLDRCEREYEGDAVI
                     LASGGFAGDPELVGRHIIGGPGRMRVRAHPRSTGDGFRAALRKGALASAGLDEFYGRN
                     LPAPPADFPPERFVEVSQLYGRYAVAVNAGGERYADEGADWSETALTRATAHQTGLYA
                     WYVLDARGLEGRVRERTAREMVETARSTGGTVIEAASLTELADRLAERGVPRDTFLRT
                     LKGYNAAVAAGEDPSPPRSGQAWPLRVPPFVAVKVAPSITHTVGGLAVDKGCRVLRGT
                     DERPIPGLYAAGVEVGGVSAGGYTSGLASALVFGNTAAESAVADG"
     gene            1275..2864
                     /locus_tag="VN178_07405"
     CDS             1275..2864
                     /locus_tag="VN178_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWS80836.1"
                     /translation="MANIGDYERTYADFDWETPERFNFGRDVVDRWAAQDRAAMVWLG
                     TSGEERRLDFAQFSVLSNRFANAARALGVGRGDRVMVLLGKVPEWHVILTGLLKLGAI
                     TIPCAPQLRTGDLKFRAEHSGSVAMISGPEGIEAVEKMRADAPSLEHFISLGEERDGW
                     ESYEALMEEASEDFTAEDTAANEGAFILYTSGTTKNPKGALHTHGYTHAAWMQARYWL
                     DLRDGDRLWCTSGTGWAKSIWNIFLGPWSQGTEIFFHEGGFDPAERLELMELYDISVL
                     CQAPTEYRLLAKTPELERANLASIRHAVSAGEPLNPAVIERWEELHGLTIYDGYGQTE
                     NTLLVGNYPGLDLRPGSMGKPSPGCDVRIIDPDGNECQPDEPGDVALFGRIPALLEEY
                     WEQPDATESVFRNGAYLTGDRAYRDADGYLWFVGRSDDVILSAGYRIGPFEVESIIIE
                     HPAVVESAVVPLPDEDRGSIVKAFVVVGEDYEPSEELAQEIQVYCKENTAPYKYPRSI
                     EFIDDLPKTTSGKIRRVELRQ"
     gene            2985..3344
                     /locus_tag="VN178_07410"
     CDS             2985..3344
                     /locus_tag="VN178_07410"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWS80837.1"
                     /translation="MLDLRALAEESFCYLTTTGRVTGRPHEIEIWFALLPETRTLYML
                     SGGGDRSDWVKNLRRNPKVTLRIADGRFAGLARGARDTEEDELARRLLVEKYESRPGS
                     LESWRRRALPVVVDLTQ"
     gene            3299..3877
                     /locus_tag="VN178_07415"
     CDS             3299..3877
                     /locus_tag="VN178_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HWS80838.1"
                     /translation="MAPQGAARGGRSHAVGAIRENRGEDTVLQSRAASPEDAEAIVRI
                     YNEGIEERIATFETSPRTAREIQAWFDGTHPVVVVEEEGRVISFANTTAYSPRACYSG
                     VAEFSVYTAGEARGRGAGTLAMKALMRAAEEAGFWKLLARVFVENEPSRKLLLSLGFR
                     EVGVHEKHARLDGVWRDVVVIERLLPANAQGR"
     gene            complement(3874..4818)
                     /gene="meaB"
                     /locus_tag="VN178_07420"
     CDS             complement(3874..4818)
                     /gene="meaB"
                     /locus_tag="VN178_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWS80839.1"
                     /translation="MDDKDGLVERLLAGDRRALARVISKIEREDEETAGIIAEIYPET
                     GDALTVGFTGPPGVGKSSVIAKLIELYRQEDRRVGVVSVDPSSPFSKGAILGDRIRLS
                     DHFLDSGVFIRSMGSRGHLGGLAGGSRLAAIAMEACGYDVILYETVGVGQGEVEVASA
                     ADTVVLCLQPGTGDSVQALKAGVMEIADIFCINKSDNPQAKNAASEVRSILEIGHELD
                     PDPWFPPIVMTRGDTGEGVEELKETVAKHRRFLEQDGRLGKRRRAALRDFVISWATNR
                     LEKEMHERLAREDTGLVEKVYNRELDPISASDRIFEDV"
     gene            complement(4833..5330)
                     /locus_tag="VN178_07425"
     CDS             complement(4833..5330)
                     /locus_tag="VN178_07425"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWS80840.1"
                     /translation="MDLRSVQRPLKKKYRNEPGSSRITLEAKASQQDTPISCSVDVGQ
                     AIYQAEAHAGVGGTGEAACSGDLLLGALAACAQVTCQMVAEAMGIEAESIEVTVTGEM
                     DLAGTLGVSEDVPVGFETIRTTFDIVAPEATEQQLEDLRQKTEQYCVVFQTLADSPDL
                     QTEWA"
     gene            5439..5678
                     /locus_tag="VN178_07430"
     CDS             5439..5678
                     /locus_tag="VN178_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80841.1"
                     /translation="MIQIRNVPDELHRTLKVRAAKAGMTLSDYLLSEIEQVAEKPTLA
                     EMMERLRSREPVELDEPPDVTIRRFRDAGDRREVD"
     gene            5685..6083
                     /locus_tag="VN178_07435"
     CDS             5685..6083
                     /locus_tag="VN178_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWS80842.1"
                     /translation="MIVLDASAMVAILVGSGPGAERIREKVEAPGESLHVPHVMELEV
                     LHALRRLALLDALSRERSSEALTDLGNISFARYPHAPLIGRIWELRENLTAYDAAYLA
                     LAEALDAPLITMDAGLARAPGIRAAVEVYG"
     gene            complement(6122..7405)
                     /gene="odhB"
                     /locus_tag="VN178_07440"
     CDS             complement(6122..7405)
                     /gene="odhB"
                     /locus_tag="VN178_07440"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HWS80843.1"
                     /translation="MAAEIHVPELGESVADATVGRWLKQEGEAVKSGDPLVELETDKI
                     NFEVEAEQDGVLQSISKDEGETVNVGEVIGTIGEGGEAGDGAAPEPQAEEEREERPQA
                     EQPEAEETGGEEQDGHREVEDGAGRASPSVRKLAQEYEVDLAEVTGSGSGGRITRDDV
                     ERLIREQSRPAAPERTEEPEHDGEAQPAPSADGRAELEERVRVSRRRQTIAQRLVESQ
                     QTTAMLTTFNEVDMSAVMELRKRRKESFQERTGARLGFMSFFTKAAIGALKAFPKVNA
                     ELQGSELLLKKYYDIGVAVSTDEGLVVPVIRDADHKNFAEIEQGIADLATKAREGKLT
                     LEDLRGGTFTITNGGIFGSLVSTPILTLPQVAILGMHKIQERPVAIEGEVVVRPMMYL
                     ALSYDHRVIDGAEAVSFLVRIKELVEDPESLLLEG"
     gene            complement(7429..>7609)
                     /locus_tag="VN178_07445"
     CDS             complement(7429..>7609)
                     /locus_tag="VN178_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80844.1"
                     /translation="LPVLYVGKPPRPSPAQGSANFHKQEHAEIVRSAFENVGQDFDEV
                     QAVEETQVRASAPSE"
BASE COUNT         1315 a         2425 c         2477 g         1392 t
ORIGIN      
        1 accgcaggga ggcgcccggc gggaacgcct ccctgcagag gctcattctc gaacgcctcg
       61 agtccgggct gacctggctg gagcatagag gaggcgcgct cctcgcgcgc gagacgggca
      121 atcccctaac cttcggcgcc cgcttcgacc ccgaacgaac ggtctcggcg ctcgtcgatc
      181 gcatagaggc cgcaggaggc gagattatcg ctgacagcgc gttggagacg ctggtggagg
      241 acccggctgg gcgcgtaaca ggtgtgcttg accggtgcga gagggagtat gaaggcgacg
      301 ccgtgatcct cgcctctggc ggcttcgcgg gcgaccccga gctggtcggg cgccacatta
      361 tcggggggcc cggaaggatg cgggtccggg cgcatccgag gagtacgggt gacggttttc
      421 gggcggcgtt gcggaagggc gctctcgcga gcgccggtct cgacgagttc tacggccgca
      481 acctgcccgc tcccccggcc gattttcctc ctgaacggtt cgtggaggtc tcccagctct
      541 acggccgcta cgccgtggcc gtcaacgccg gcggcgaacg ctacgccgac gagggcgcgg
      601 actggtccga gacggccctg acacgggcca cggcgcacca gactggactc tacgcctggt
      661 atgtcctcga cgccagagga ctcgaaggcc gcgtgcggga gcggaccgcg agagagatgg
      721 tcgagacggc aaggagcacg ggcggaacag tcatcgaagc tgcgagcctc acggagcttg
      781 cggacaggct cgccgaacgc ggcgtgcccc gcgatacctt cctccgcacc ctgaaagggt
      841 ataacgcagc cgtcgccgcc ggtgaagatc cctccccccc gaggagtggc caggcctggc
      901 cattgcgcgt tcctcccttt gtggccgtaa aggttgcgcc atccataacg catacggttg
      961 gcgggctcgc cgtcgacaag ggatgcaggg tactgcgcgg gactgacgag aggcccatcc
     1021 cgggcctcta cgccgctggg gtcgaggtcg gcggggtctc cgccggtggg tacacgagcg
     1081 gcctcgcctc tgctctcgtc ttcggcaata ccgccgcgga gtcagcggtc gcagacggat
     1141 aggagtattt cagattgtcg gcacttcgtt atccaaggag gtggatggac gattcgcagt
     1201 aggctattac cccgctaaat cgctttagta caatgcggtt ccggaaagcg acctttgggg
     1261 aaaggtggtc gatcgtggcc aacatcggcg attacgagag gacctacgca gatttcgact
     1321 gggagacgcc tgagaggttc aacttcgggc gtgatgtggt ggaccggtgg gccgcacagg
     1381 accgggcggc catggtctgg ctgggaacga gcggtgagga acgccgcctc gacttcgccc
     1441 agttctccgt actctcgaac aggttcgcca acgcagctag agcgttgggc gtggggcgcg
     1501 gcgacagggt gatggtccta ctcggcaagg tgcctgagtg gcatgtaatc ctgacgggcc
     1561 tcttgaagct gggggccatt accatcccgt gcgccccgca gctcaggacc ggcgacctga
     1621 agttcagggc cgagcactcc ggctctgtgg ccatgatctc gggccccgaa ggcatcgaag
     1681 cggtcgagaa gatgcgcgcg gacgccccaa gtctggaaca cttcatctct ctgggtgaag
     1741 agcgcgacgg ctgggaatcc tacgaggcct tgatggaaga ggcctctgag gacttcacgg
     1801 ccgaggacac agcggcgaat gagggcgctt tcatcctcta cacttcgggg acgacgaaga
     1861 atcccaaggg cgccctgcac acccacggtt atacgcacgc cgcgtggatg caggccaggt
     1921 actggctcga cctcagggat ggcgacaggc tgtggtgcac ctcggggacg ggctgggcca
     1981 agagcatctg gaacatattt ctggggccgt ggagtcaggg gacggagatc ttcttccacg
     2041 agggcggttt cgaccctgcc gaacgcctgg agctgatgga actctacgac atctctgtcc
     2101 tctgccaggc cccgaccgag taccggctcc tcgccaagac cccggaactg gaacgcgcga
     2161 acctcgcctc catccgccac gccgtctccg ccggagaacc gctcaacccg gcggtcatcg
     2221 agcggtggga agagcttcac ggcctgacga tctacgacgg ttacgggcag accgagaaca
     2281 ccctgctcgt cggcaactat cccgggctcg acttgcgacc gggctcgatg ggcaaaccgt
     2341 ctcctggctg cgacgtgagg atcatcgacc cagatggcaa cgagtgtcag cctgatgagc
     2401 ctggcgatgt agccctcttc gggcgcatac ccgcgttgtt ggaagagtac tgggagcagc
     2461 cagatgcgac cgagtcggtc tttcgtaacg gcgcctactt gaccggtgac agggcttaca
     2521 gggacgcgga tggctacctc tggttcgtgg gccgttcgga cgacgtgatc ctctctgccg
     2581 gttaccgcat cggacctttc gaggtggaga gcatcattat cgagcatccc gcggtagtcg
     2641 agagcgccgt cgtgcctctg cccgacgagg acagggggag catcgtcaag gcgttcgtag
     2701 tcgttggcga agactacgag ccctcggagg agctggcgca ggagatccag gtctactgca
     2761 aggagaacac ggccccgtac aagtacccgc gcagcataga gttcatcgac gacctaccca
     2821 agacgacgag cggcaagatc cgtcgcgtcg agctgcgcca atgaaggcat caggtctcag
     2881 gcatcaggct tcaggtgagg gtctctcacg gcacgtctgc tttccgaacg gatctacagc
     2941 ccgtggcccc agatctgaaa cctgtagcct gagacctgat gcctatgctt gatcttcgcg
     3001 ccctcgccga agagagcttt tgctacctga cgacgacggg gagggtaacg gggaggccgc
     3061 acgagatcga gatctggttc gctctactgc cggaaacgcg taccctctac atgctctccg
     3121 gaggcggtga ccgctcggac tgggtgaaga acctccgccg aaacccgaag gtcacgctcc
     3181 gtatcgcaga cggacgtttc gccgggctgg cccgcggggc aagagacacg gaagaggatg
     3241 agctggcccg gcggctgctg gtcgagaagt acgagagccg tcctggaagt ctggagagtt
     3301 ggcgccgcag ggcgctgccc gtggtggtag atctcacgca gtaggggcca tccgggagaa
     3361 tcgaggtgag gatacggtcc tacagtctag agcagcgagc ccggaagacg cggaggccat
     3421 agtccgcatc tacaacgaag gcatcgagga gcgcatcgcg acgttcgaga ccagcccgcg
     3481 tacggcacgg gagatacagg cctggttcga tggaacccac cctgtcgtgg tggtggagga
     3541 agaaggacgg gtgatctcct tcgccaacac cacggcctac agcccccgcg catgctactc
     3601 cggcgtggcc gagttctccg tatacaccgc cggcgaggca aggggccgtg gggcgggcac
     3661 gcttgcgatg aaggccctca tgcgggcggc ggaggaggct ggcttctgga aactcctcgc
     3721 gcgggtcttc gtggagaacg agccgagccg gaagctgctg ctctccttgg gcttccggga
     3781 ggtgggagtc cacgagaaac acgccaggct cgacggcgtg tggcgtgacg tcgtagtcat
     3841 cgagcgactg cttccagcaa acgcgcaggg gcgctagaca tcctcgaaga tgcgatccga
     3901 ggccgagatg gggtcgagtt ccctgttgta gaccttctcc acgagccccg tatcctcgcg
     3961 ggcaaggcgc tcgtgcatct ctttctccag gcggttcgtg gcccacgaga tgacgaagtc
     4021 cctcagggcg gcccgccgcc tctttccgag cctgccgtcc tgttcgagga accgacggtg
     4081 cttcgctacg gtttctttga gttcctctac gccttcgccc gtatcgccgc gggtcatcac
     4141 gatgggcggg aaccacgggt cggggtcgag ctcgtgtccg atctccagta tggaacgcac
     4201 ctctgaggcg gcgttcttgg cctgcggatt gtccgacttg ttgatacaga agatgtcggc
     4261 tatctccatg accccggcct tgagcgcctg caccgagtca cccgttcccg gctgcaggca
     4321 gagcacaacc gtgtccgcgg ccgaggcgac ctcgacctca ccctgcccta ccccgacggt
     4381 ctcgtagagg atcacgtcgt agccgcaagc ctccatcgcg atggcggcga gtctcgagcc
     4441 gccggcgagc ccaccaaggt gcccgcggct acccatcgag cggatgaaca caccggagtc
     4501 gaggaagtgg tcggagagcc ttatgcggtc accaagaata gcacccttcg agaagggact
     4561 cgaagggtcg acagagacca ccccgacccg cctgtcttcc tgcctgtaga gctctatgag
     4621 cttcgcgatg acgctgctct tgccgacgcc gggcggcccg gtgaagccta cggtcagagc
     4681 atcgccggtc tcggggtaga tctctgcgat tatcccggcc gtctcttcgt cttcgcgctc
     4741 tatcttcgag atcacccgcg ccaacgcacg cctgtcgccc gcgagcaacc gttctacaag
     4801 cccatccttg tcgtccaagc cggtccgatc ttctatgccc actccgtctg gaggtccggc
     4861 gagtcggcca gcgtctggaa gacgacgcag tactgctcgg tcttctggcg caggtcctcc
     4921 aactgttgtt cggtagcctc gggcgccacg atgtcgaacg tggtgcggat ggtctcgaag
     4981 ccgacgggga cgtcctcgga gacgccgagc gtgccggcga gatccatctc ccccgtaacc
     5041 gttacctcga tgctttccgc ctctatcccc atggcctccg cgaccatctg gcaggttacc
     5101 tgggcacagg ccgccaacgc tcccaacaga agatccccgg agcaggccgc ctcgcccgtt
     5161 cccccgactc cggcgtgggc ttcggcttgg tagatcgcct gaccgacatc cacggagcag
     5221 gagatcgggg tgtcctgctg gctcgctttg gcttcgagcg ttatccggga tgaccccggc
     5281 tcattgcgat actttttctt gagcggcctc tgcaccgacc gaagatccat gttcatcctc
     5341 ctgtggtagt cgcccttcca cagcgtattg tagccgcctg acagcgaaag ataataaaca
     5401 tgcgcaacac atgtgttact cttttccgca tgtcgaagat gatacagata aggaacgtac
     5461 ccgacgagtt gcaccgcacg ctgaaggtca gggcggcgaa ggccggcatg acgctctctg
     5521 actacctcct ttcggagata gagcaggtgg ccgagaagcc gaccctcgcc gagatgatgg
     5581 agcggctacg cagcagggaa ccggtcgaac ttgacgagcc gccggacgtc acgatacgcc
     5641 ggttcaggga tgccggcgat cgtcgcgagg tcgactagcg gactttgata gtcctcgacg
     5701 cctcggcgat ggtagccata ctcgtgggtt cggggccggg ggccgagcgc atccgggaga
     5761 aggtagaggc tcctggcgag agcctccacg tcccccatgt catggaactg gaggtgctgc
     5821 atgcgcttcg caggctggcc ttgctcgacg ctctctctcg ggaaaggagc tccgaggccc
     5881 ttaccgactt ggggaacata tcgttcgcgc gctacccgca cgcgcccttg atagggagga
     5941 tctgggaact cagggaaaac ctcaccgcat acgacgcggc ttacttagcc ctcgccgagg
     6001 cgctagacgc gccgctgatc acgatggacg ccggacttgc gcgggcccct ggcatccgcg
     6061 ccgcggtcga ggtctacggg tagacgaagg ggctcccgaa cggaagcccc tgaccgtgtg
     6121 cttagccttc cagcagcagc gactcggggt cttcgaccag ttctttgatg cggaccagga
     6181 agcttaccgc ctcggcgccg tctatgacgc ggtggtcgta ggagagcgcg aggtacatca
     6241 tgggacggac cacgacctcg ccttcgatgg cgacggggcg ctcctggatc ttgtgcatcc
     6301 ccagtatcgc gacctggggc aaggtgagga tgggcgtgga gactagcgag ccgaagatgc
     6361 caccgttggt tatggtgaac gttccgccgc gcaggtcttc cagggtcagc ttgccctcac
     6421 gggcctttgt ggccaggtcg gctatgccct gttcgatctc cgcgaagttc ttatggtccg
     6481 cgtcgcggat gacggggacg acgagcccct cgtccgtgga gacggctacg ccgatgtcgt
     6541 agtatttctt gaggaggagc tcgctcccct gcaactcggc gttgaccttg gggaaagcct
     6601 ttagcgcccc gatggcggcc ttggtgaaga agctcatgaa gccgagccgc gcccccgtcc
     6661 gttcctggaa agactccttg cggcgcttcc tcagctccat gaccgcgctc atgtcgacct
     6721 cgttgaaggt ggtgagcata gccgtcgtct gctgcgactc gaccaacctc tgggcgatgg
     6781 tctggcgcct gcgggagacg cgcacccgct cctccaactc cgccctgcca tccgcagaag
     6841 gggccggctg cgcttcgccg tcgtgctcgg gttcctcggt ccgctcgggg gccgccggtc
     6901 gggattgttc ccggatcaga cgttccacat cgtcgcgggt gatgcgtccg cctgaaccgc
     6961 tgccggtgac ctcggcgagg tcgacctcgt actcctgggc caactttcta acggaaggcg
     7021 aggcgcggcc ggccccgtcc tcgacctccc tgtgcccatc ctgctcttcg ccaccggtct
     7081 cttcggcctc gggctgctcg gcctgcggac gctcctcccg ttcttcttcg gcctgtggct
     7141 cgggggcggc gccgtcgccg gcttcgcccc cctcgcctat ggtgcctatg acctcgccga
     7201 cgttgacggt ctcgccctcg tccttggaga tggactggag gacgccgtct tgctcggcct
     7261 cgacctcgaa gttgatctta tccgtttcga gctcgacgag cggatcgccc gactttacgg
     7321 cctcgccctc ttgcttgagc cagcgcccga ccgtcgcgtc ggcgaccgac tccccaagct
     7381 cgggtacgtg tatctcagca gccactgctc actcttcctc tctgactctc actcgctcgg
     7441 ggcggaggcg cggacctgcg tctcctccac ggcctgtact tcatcgaagt cctgcccgac
     7501 gttctcgaag gccgacctca cgatttcggc gtgctcctgc ttgtggaagt tggccgagcc
     7561 ctgggcggga cttgggcgcg ggggctttcc gacgtagagt acgggaagc
//
LOCUS       DATMVF010000146         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_521540, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000146 DATMVF010000000
VERSION     DATMVF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521540"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1207)
                     /gene="nagA"
                     /locus_tag="VN178_07450"
     CDS             complement(53..1207)
                     /gene="nagA"
                     /locus_tag="VN178_07450"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HWS80845.1"
                     /translation="MAEAYALVGEIFTASGPTRAAIVVRNGKIDALLRDPRSGDLPRE
                     HREFAGLLCPGFIDLQINGAFGADIGPDPNAIRTLVTKLPTTGVTSFLPTLISSPPER
                     YEEFFDTLEEGAIARGARVLGAHLEGPFLAPERKGAHDLANLRPIDLGFLRELLRSGK
                     VKVMTLAPELLNSLQAIELLLEEGAVASAGHTAATYEEIIRAAEVGLSQGTHLYNAMR
                     PLAHREPGTVGALLTDDRLRVGIIPDGVHVHEAALRLAYRAKGSERLILVTDAMEAAG
                     MAPGDYELGGRRVHLEDGAVRLSDGTLAGSALTMDEAVRNAAQFLGVPLTEAVRMASE
                     VPALALNLKGKGRIAAGCDADFVLLDARGTVLETIVGGKTVYDREENEHP"
     gene            complement(1222..1434)
                     /locus_tag="VN178_07455"
     CDS             complement(1222..1434)
                     /locus_tag="VN178_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80846.1"
                     /translation="MSDVYELADLLKANGSAAVSKGFEGQPAVIKGADHLLSEIFVCS
                     GGHARTVIGDPLPDGPPVELVVSLGA"
     gene            complement(1431..2666)
                     /locus_tag="VN178_07460"
     CDS             complement(1431..2666)
                     /locus_tag="VN178_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWS80847.1"
                     /translation="MSELRDALRENLAQARDDLEPELIEVRRDFHREPELRFEEHRTS
                     ETVGELLGSLGLSVRSGVAGTGLLADLHGKRPGPTVALRADMDALPIRDAKGAPYASR
                     REGVMHACGHDAHMTIAYGVLRVLEPLRAILPGTLRVIFQPGEEVPAGEKSGALEVIR
                     AGALEDPEVEAIFGLHVWPELPADTVGLQPGVTMAAADSFIVEVRGESSHAGEPHKGK
                     DAIFAASSLVVQLKALLGREIPPAEPATINVGTIRGGASQSVVADLVELSGTLRSLGG
                     DRRERLLQSMQRVAGGTSSTTGCSVRLNVSDSFPAVVNDPEHYERALEILLETLGSDR
                     VRVLTDVPMTADDFAHYLEAVPALYIKLGCAPAEGNVYPLHHHCFDIDERVIWTGVEA
                     VSSILLDTMEARVAEEICA"
     gene            complement(2663..>3069)
                     /locus_tag="VN178_07465"
     CDS             complement(2663..>3069)
                     /locus_tag="VN178_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80848.1"
                     /translation="ARRNGFCPDDYRLDPCKVDVPLGGNEKLRCGDLDLAVTPTPGHS
                     AGSVCFLVRIEEGLALFAGDTVFAGGQISLIVAPGSDLLALQESVTSLGGLGVASLLP
                     GHGIFPLEGGQEHIDQAIEAFGTMLPPRSILQ"
BASE COUNT          550 a         1034 c          922 g          563 t
ORIGIN      
        1 gcttgcgccg gtcgtctcct ccctaacaca ggcactgatg ccacgtaaag attcatggat
       61 gctcgttctc ctcacggtcg tagacggtct ttccgcccac gatggtctcg agcacagtac
      121 cccgcgcgtc caatagcaca aagtcggcat cacaaccggc ggcgatccta cctttgccct
      181 tgaggttcaa ggccaaagcg ggaacctcgg aggccatgcg cacagcttca gtaaggggga
      241 caccgagaaa ttgagcggcg tttcttacgg cttcgtccat ggtgagcgcg ctcccggcga
      301 gtgtcccatc ggaaagccgc accgcgccat cttccagatg aaccctccgc ccccccaact
      361 cgtagtcgcc cggggccata cccgctgctt ccatggcatc cgttaccagg atcagacgtt
      421 cagagccctt tgcccgataa gcgagacgaa gtgccgcctc gtggacgtgc acaccgtccg
      481 ggatgatgcc taccctcagg cggtcgtcgg taagcaaggc gcctacggtg ccgggttccc
      541 tgtgagccag cggtctcatc gcattgtata ggtgggtacc ctggctcagg ccgacttccg
      601 cagcccggat aatctcttca tatgtggccg cggtgtgacc ggcactggcg acggcgcctt
      661 cctccaggag aagttcgatc gcctgcaatg agttcagcag ctcgggggcc agagtcataa
      721 cctttacttt ccccgaacgg agaagttccc tcaggaaacc cagatctata ggtcgcagat
      781 tggcaaggtc gtgcgccccc ttacgttccg gggcaaggaa tggtccctcg aggtgtgctc
      841 ctagtacacg ggcaccgcgc gctatcgcgc cctcttcgag ggtgtcgaag aactcctcgt
      901 agcgctccgg tggggaggag atcagcgtgg gaaggaaaga agttacccca gtagtcggta
      961 gtttggtcac cagggtcctg atcgcgttcg ggtccggtcc tatgtctgcg ccgaacgcgc
     1021 cgttgatctg caagtctatg aagcccgggc agagcaaccc ggcgaattcc cggtgctctc
     1081 tgggaaggtc tccggatcgc gggtctcgaa gcagagcatc gatcttgcca tttcggacca
     1141 cgatcgctgc ccgagttggc cccgatgcag tgaagatttc cccgaccagc gcgtaggcct
     1201 cagccattac atcccctaca gctaagcacc caaagaaacg acgagttcca ccggtgggcc
     1261 gtccggcaag gggtccccga tcaccgttcg ggcgtggccg ccggagcata cgaagatttc
     1321 gctcaacaaa tggtccgccc cctttatgac ggccggttgc ccctcgaatc ctttgctcac
     1381 cgcagcggag ccgttcgcct tcaggaggtc agccagttcg tagacgtcgc tcacgcgcat
     1441 atctcctcgg ccactcgggc ttccatcgtg tcgagcagaa tcgaggagac ggcctcgacg
     1501 cccgtccaga taacccgctc atctatgtcg aagcagtggt ggtgcaaggg gtagacgttc
     1561 ccctccgccg gtgcgcaacc cagctttatg tacagggccg gcaccgcttc gaggtagtgg
     1621 gcgaagtcgt ccgcggtcat cggcacgtca gtcagaacac gcaccctgtc gctaccgagc
     1681 gtctcgagga ggatctccag cgcccgctcg tagtgctccg ggtcgttgac aactgccggg
     1741 aaggagtccg agacgttcag gcgcacggag caccccgtag tgctggaggt tcctccggcc
     1801 acccgctgca tactctgcag gagacgctcc cggcggtccc cgccgaggct gcgcagggtt
     1861 ccggagagct cgacgagatc cgctaccacg ctttgtgacg caccgccgcg gatcgtcccg
     1921 acgttgatgg tggcgggttc tgcgggcggg atctcacgcc cgaggagcgc cttgagctgg
     1981 acgaccagcg agctggccgc gaagatggcg tccttgcctt tgtggggctc cccggcatgg
     2041 gacgactccc cgcgcacctc cacgatgaag gagtcggcag cggccatcgt cacccctggc
     2101 tgcaggccaa ccgtgtcagc cggcaactca ggccagacgt ggagaccgaa gatcgcctcg
     2161 acctccgggt cctccagtgc cccggctctt atgacctcca gggctccgct cttctccccg
     2221 gccggcacct cctcgcctgg ctggaagatc accctgagcg tccccggcag gatggcccgg
     2281 agtggctcca ggacgcgaag cacgccgtag gcgatcgtca tgtgcgcgtc gtgcccgcaa
     2341 gcgtgcatca ctccttctcg ccgggaagcg tacggagcgc ctttcgcgtc acggatcggc
     2401 agcgcgtcca tgtccgcccg gagggctacg gtcggaccgg gtcgcttgcc gtgcaggtcc
     2461 gccagcaggc ccgtccccgc aaccccggac cgcacggaga gccccaggga gcctagaagt
     2521 tccccgacgg tctctgaggt acggtgctcc tcgaagcgca gctcgggttc cctgtggaaa
     2581 tcgcgccgga cctcgatcag ctcgggttcc agatcatcgc gtgcctgcgc cagattctcc
     2641 cgcaatgcat cccgtagttc gctcattgca gtatcgacct cggcggcagc attgtcccga
     2701 aggcttctat ggcctgatct atgtgctcct gccctccctc caaggggaag atgccgtgac
     2761 caggaagcag gctcgccaca cccaatcctc cgagactggt taccgactcc tgcagggcga
     2821 ggaggtccga accgggggcc acgattaggc tgatctgccc tccggcgaaa accgtgtctc
     2881 cggcaaacaa ggctaatccc tcctcgatcc tcacgagaaa gcaaacggag ccggcgctgt
     2941 gacccggcgt cggcgtcacc gccagatcca gatccccgca ccttagcttc tcgtttcccc
     3001 cgagcggcac gtccaccttg cagggatcga gccggtagtc atcgggacag aaaccgttcc
     3061 gtcgggcga
//
LOCUS       DATMVF010000147         4825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_14907, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000147 DATMVF010000000
VERSION     DATMVF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14907"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..984
                     /locus_tag="VN178_07470"
     CDS             550..984
                     /locus_tag="VN178_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWS80849.1"
                     /translation="MRRLTLLVALVLAIVLIAAVPVVAAQRATLEASLTGDKEVPGPG
                     DPNGRGEADIKVYKAKVCYELEVERIKPANAAHIHRGGPSVAGPVVVELKAPMDGSSE
                     GCKAISNKLSKKLREHPSRYYVNVHNEPYPDGAVRGQLHSND"
     gene            1351..3621
                     /locus_tag="VN178_07475"
     CDS             1351..3621
                     /locus_tag="VN178_07475"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWS80850.1"
                     /translation="METINEKGQLVRVEIPVTGMTCAGCVGRVERAIEKVPGVLEANV
                     NLASEKATVRYVAGEVERRELQRAVEGAGYGLARGEEHPGESEYEKLKDDFLLAAALT
                     ALIFAGSLPMMLGLEPLVPMMWLSFVLLGLATPVQFWAGRRFYRGAWGALEHGQANMN
                     TLVVMGTSAAYLYSAVATLAPGLFAAGRADVYFDTSALIISLILLGRLLEARAKGRTD
                     EAIKKLAGLRAKTARVVRGGEEFDVSVEDVRAGEIVVVRPGEKVPVDGRVVAGGSAVD
                     ESMITGESLPVAKREGDEVIGATMNTSGSLRFEATKVGEDTALHQIMRMVEEARGSKA
                     PIQRLADRISAVFVPAVIGVAAVTFSIWLLFGPEPTLTFALLNTVAVLIIACPCAMGL
                     ATPTSIMVGTGKGAESGILVRGGESLEGAHKLDTVVLDKTGTLTRGEPELTDIVLTNG
                     IPEEELMRLLASAELGSEHPLGEAIVRGARDRGLPPTEADAFEAISGGGIRARVEGRE
                     VLVGSRRFLSASGLSEDGLAARSEELAREGKTPIFIAVDGEPAGLVAVADVIRDEARE
                     AVEQLYALGLEVTMLTGDDRRTAGAIARQLGIERVVAEVRPEDKADEVKRLQSEGKRV
                     GMVGDGINDAPALAQADVGIAIGTGTDVAMEAADLTLISGDVRGVARAIGLSRATVRN
                     IKQNLFWAFAYNVALIPVAAGILYPLFSDGSVPWVLSPVLGEYGFLNPVLAAAAMALS
                     SVSVLSNALRLRRAKV"
     gene            3637..3966
                     /locus_tag="VN178_07480"
     CDS             3637..3966
                     /locus_tag="VN178_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWS80851.1"
                     /translation="MDATRKSDANPAEGTAHGYIKAKDKERLENRLRRIEGQVRGVQR
                     MVDEEAYCVDVLTQIASVVSALEKVGTILLKDHVEHCVRESIEKGEDADEKIEELTTA
                     VERFLRV"
     gene            3992..4201
                     /locus_tag="VN178_07485"
     CDS             3992..4201
                     /locus_tag="VN178_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWS80852.1"
                     /translation="MTGKTLSVEGMGCGHCKAAVKGELNKLFGVESSNANVEEGTVEI
                     NYDEARVTAKDLEAAIEEAGYTVAA"
     gene            4371..>4825
                     /locus_tag="VN178_07490"
     CDS             4371..>4825
                     /locus_tag="VN178_07490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS80853.1"
                     /translation="MSDKFHEGHREGRGDVEAERFWEDHYRRRGRVWSGRPNPVLVDV
                     VGSLPPGRALDLGCGEGGDAIWLARQGWRVTAVDVSATALDRAAEDAAKAGVADRIDF
                     QRHDLAVNFPSGVFDLVSAQYLHSPVEFPRVHVLQEAASAVTRGGLLLIV"
BASE COUNT          946 a         1390 c         1697 g          792 t
ORIGIN      
        1 tcacgccctc gtagcgccgc actgcaacgt acatagcttt agccttcctc cccttaagag
       61 aggacgggac ccgtcgaccc cgccttgagt tgatcatcgt gcccagtgtg aaaggttaga
      121 agggctggcg caacttccga gaccgcgtga aagcggaagt tcaccttcgg tgaaccgtgt
      181 tctagacaca cttcggtcaa tagcttaaag tgttcgaggg atctcgagcc ggcgcggttc
      241 tgcaggaaag tagcgcgctc gacgagttcg cctgaggctt cgtcgtctaa cctcttctga
      301 gccactgtgc acgccggcgt actctacggc gggaggcaag ccgagaacaa gccgacctat
      361 cttacgggcg acaggaagtt cgcgagcgat ccccatatca gaagcttagc ggctgcagac
      421 caccgagctt ctgcccggag ctaaacaagt cggatgttcg cagggaaggt tgatccatat
      481 gatccaccgt tgcgtacaaa tcggtgtagg tgtggagagg ctccccaacc aaaggaggta
      541 gagagcccga tgaggagact gaccctgctg gtagcgctcg tcctggcgat tgtactgata
      601 gcggccgtcc ccgtggtggc cgcccagcga gccacgctcg aagcatcctt gacgggcgat
      661 aaagaggtgc cgggcccggg cgaccccaat ggccggggtg aggccgatat caaggtctac
      721 aaagcgaagg tgtgctacga gctcgaagtg gagcgcataa agccggccaa cgcggcccat
      781 atccatcggg gaggacctag tgtggccggc ccagttgtcg tggagctcaa ggcgcccatg
      841 gacggctcct cagaaggctg caaggcgata agcaacaagc tgagcaagaa gctaagggag
      901 cacccctcac gctattacgt caacgtccac aacgaaccgt accccgatgg tgccgtaagg
      961 ggccagctgc acagtaacga ctagggtcgc acgaggttag acccacgagg taagaagggc
     1021 cgggattgta ggtcccggcc cttctcttct tgcccttatt cacccaacat tgggaaaggg
     1081 taattctacg gcgtccatgt gcaggatgct gcatagctct gttcgcaggc catcctctgg
     1141 cactctgaaa actcttctct cctcagggcg gaccgctaca tgctgccatc cgcatgacta
     1201 tgcactcaga tgcgaatgcg acgcaccgtc gagactcgaa tacccacact ggggtaggca
     1261 ggagtccaca ggcaaagcca gattgacgtg tagggtaggg gggtatagca taaggccggc
     1321 tcgaacgagc gcgaagtgag ggagttcggc atggagacga tcaacgagaa ggggcagctg
     1381 gtgcgggtgg agatccctgt gactggcatg acgtgcgcgg gttgcgtggg gcgggtcgag
     1441 cgggctatcg agaaggtgcc tggcgtgctg gaggcgaacg tgaacctcgc cagcgagaag
     1501 gctacggtcc ggtacgtcgc gggcgaggtg gagcgccggg agctgcagcg ggccgtcgag
     1561 ggggctggct acggtctggc ccggggggag gagcaccctg gcgagagcga gtacgagaag
     1621 ctcaaggatg atttcctgct ggcggcggcg cttaccgcac tgatcttcgc tggcagcctg
     1681 ccgatgatgc tcggcttgga gccgctggta cccatgatgt ggctgagctt cgtgctcctc
     1741 gggcttgcga cgccggtgca attttgggcc gggcggcggt tctatcgggg ggcttggggc
     1801 gcgctcgagc atgggcaggc gaacatgaat acgctggtgg tcatgggtac tagcgcggcg
     1861 tacctctaca gcgctgtggc gaccctggcg ccggggctct tcgcggctgg cagggccgac
     1921 gtgtacttcg acacctcggc gctcattatc tccctgatcc tgctggggcg attgctcgag
     1981 gcgcgggcca agggacgcac cgacgaggcg ataaagaagc ttgccgggct gcgggcgaag
     2041 accgcccgcg tcgtgcgcgg tggggaggag tttgacgtga gcgtcgaaga cgtgcgggcc
     2101 ggtgagatcg tcgtggtcag gccgggggag aaggtgccgg tggacggacg ggtcgtcgcc
     2161 ggcgggtctg cggtggacga gtcgatgatc acgggcgagt ccttgcccgt tgcgaagagg
     2221 gagggcgacg aggtgatcgg ggctaccatg aacaccagtg ggtctctccg ctttgaggcg
     2281 acgaaggtcg gtgaggatac cgccctgcat cagatcatgc ggatggtcga agaggctcga
     2341 gggtcgaagg cccctatcca gcgtctcgcc gacaggatca gcgccgtgtt cgtgcccgcg
     2401 gtgatcggcg tagcggccgt gacgttctcg atctggctcc tgttcgggcc cgagccgacc
     2461 ctcacgttcg cgctcctgaa cacggtggcg gtcttgatca tcgcctgccc gtgcgcgatg
     2521 ggcctggcga cgcctacctc gatcatggtc ggtaccggca agggagcaga atcgggtatc
     2581 ctggtcaggg gcggagagtc tctcgaaggg gcccacaagc tcgacacggt cgtgctggac
     2641 aagaccggca ccctcaccag gggcgagccc gagctgaccg acatagtgct cacgaacggt
     2701 atcccggaag aggaactcat gagactcctg gcttcggcgg agttgggtag cgagcatccc
     2761 ttgggcgaag ccatagtccg cggcgccaga gaccgcggtc tcccgccgac cgaagccgat
     2821 gctttcgagg cgatctcggg cggcgggatt cgcgcccgcg tcgagggtag ggaggtcctc
     2881 gtcggtagcc gccgcttcct ctccgcgtcg gggctctcgg aggacggcct cgccgcgagg
     2941 agcgaggagc tcgcgcgcga ggggaagacc ccgatcttca tcgcggtgga cggcgagccg
     3001 gcaggcctcg tagcggtcgc ggatgtgatc cgcgacgagg ccagggaagc ggtcgagcag
     3061 ttgtacgcgt tggggctcga ggtcactatg ttgaccggcg acgaccgtcg caccgcaggg
     3121 gcgatcgcgc gccagctcgg catagagcgc gtcgttgcgg aagtgcggcc cgaggacaaa
     3181 gccgacgagg tgaagcgcct ccagtccgag ggcaagcggg taggcatggt cggcgacggc
     3241 ataaacgacg ccccggccct ggcgcaggcc gacgtcggta tagccatagg cacaggcacc
     3301 gacgtcgcga tggaggccgc cgacctgacg ctcatctcgg gcgacgtgcg cggggtggcg
     3361 cgggcgatag ggctgtcgag agccacggtc aggaacatca agcagaacct gttctgggcc
     3421 ttcgcctaca acgtcgcgct catccccgta gcagcaggga ttctctatcc tctcttctcc
     3481 gacgggagcg tgccatgggt cttgagtccg gttctcgggg agtatggctt cctcaacccc
     3541 gttctggccg cagcggcgat ggcgctctct tcggtgagtg tactctcgaa cgccctgcgg
     3601 ctgcgccggg cgaaagtata gagaaaggag acagtggtgg acgcgacacg aaagagcgac
     3661 gcgaacccgg cggaggggac cgcgcacggt tacataaagg ccaaagacaa ggaacggctc
     3721 gaaaatcggc tcaggcgaat cgagggccag gtgcggggcg tgcagcgcat ggtggatgag
     3781 gaagcctact gtgtggacgt cctcacccag atagccagcg tcgtctcggc gctggagaag
     3841 gtcggcacca tactcctcaa ggaccacgtc gagcactgcg tcagggagtc catagaaaag
     3901 ggcgaagacg cggacgaaaa gatcgaggag ctcaccacgg ccgtcgagcg cttcctgaga
     3961 gtctagaaga gaagcagaga aagggaaaaa tatgaccggc aagacgctga gcgtggaagg
     4021 catgggctgt ggccactgca aagccgcggt caaaggcgaa ctgaacaagc tcttcggggt
     4081 ggagagctcc aacgcgaacg tcgaggaggg caccgtcgaa atcaactacg atgaggccag
     4141 ggtgaccgcc aaggacctcg aggccgccat cgaggaggcc ggctacaccg tagcggctta
     4201 ggcgggtagc cgggcggatt cccggcaagg cagggatccc tgcggatcga agggagccga
     4261 ctacgtgaac gacgagcctc ggaagattgt ttcgtgagcg ccctgcaaca gtacgcgctc
     4321 tctcggcact aaactgcaaa gtagcagagc aacgcagggg agacgcaacg ttgagcgaca
     4381 aatttcacga agggcaccga gaagggcgcg gcgacgtgga ggccgagcgg ttctgggaag
     4441 accattaccg ccggcgcgga cgcgtgtgga gcgggaggcc gaatcccgtt ttggtcgacg
     4501 tcgtcggaag cctacctccg ggcagagcgc tggacctcgg ctgcggcgaa gggggagacg
     4561 cgatctggct cgccaggcag ggctggcgcg tgacggccgt cgacgtttcc gcaacggcgc
     4621 tggatcgggc ggccgaggac gcggccaagg ccggcgtcgc cgaccgcatc gacttccaga
     4681 ggcacgatct cgccgtcaac ttcccctcgg gcgtcttcga cctggtctcc gcccagtacc
     4741 tgcactcgcc ggtagagttt ccccgcgtcc acgtcctgca agaagcagct agcgccgtga
     4801 cgcgcggtgg cctcctgctt atcgt
//
LOCUS       DATMVF010000148         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_299209, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000148 DATMVF010000000
VERSION     DATMVF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299209"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="selD"
                     /locus_tag="VN178_07495"
     CDS             <1..965
                     /gene="selD"
                     /locus_tag="VN178_07495"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWS80854.1"
                     /translation="GDLEQVLSGLTPGSVDDYVSGMETRDDAGFIPFGGGLLLQTADF
                     FTPIVDDPYRFGQIAAANAFSDVYAMGGEPLTAMNLVAFPCSLDLGVLREILAGGLAK
                     IEESGARLCGGHSVQDEEPKYGLSVTGFVEEDRVVRNAGASAGDALVLTKPLGLGILT
                     TALKRDLVSDEEIEDAVRSATLLNKAAGDAMREVGVSAATDVTGYGFLGHLTEMLHAS
                     EVGADVRRDEVPVWERAVPLANEGVYPGGLKSNREYLKNRVTADGASQDDLLPLYDPQ
                     TSGGLLVAVPGNRVSALVGELDRRGVSGAVVGEVVEQPGVRVIR"
     gene            1084..2016
                     /locus_tag="VN178_07500"
     CDS             1084..2016
                     /locus_tag="VN178_07500"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWS80855.1"
                     /translation="MQSILNSALPFFALILCGYVAGRLRLLDETAAAGVNTFVFYFAL
                     PSFLFGLMSSSPIGEVVNVPFIGAYVTASVVLFALTALLGLYLFEIGRAEAAVQGLAA
                     VLANTGYMGIPLVSAVFGREAAVPVVVGLTLDGVLMIPLGIMIIESSKGRAEGPVRTA
                     LSTFSSLARNPLILSIFAGLAVSTAGLDPPTPLANFLDLLGGTAGPCALFALGATLAG
                     RTVAGGTAEISYMTFMKLALHPAALFFTTTVIFTVDPLWATAALLGAALPIAANVFIV
                     ARQYDVYVARTSSAILISTAVSMISVSALLLVLQ"
     gene            2267..3688
                     /locus_tag="VN178_07505"
     CDS             2267..3688
                     /locus_tag="VN178_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80856.1"
                     /translation="MSADEVVPGKPEVSKRLLIGVLVSAVFVSVLNSSMVNVVIPVIG
                     EDYGVTEAQVGWVITGYLLTYAVGIPLYGRAADVFGIRRTFIVGLLAFAAGSLLCAWA
                     PSLVALVFGRIVQGVGGAAIPALSSVSVTKILPAGERGAALGLIVSSVGIGAAIGPIL
                     GGAVGALVGWHYLFVGTLILTAILIPAALYVLPGDSPEEARGFDLPGGILLGLAAGLF
                     LFGITRGQVSGFGSAGSWGSFVGSVLAAFGFGWRIRNAAEPFVSPTLFRNRAYVAAVV
                     VGYFSMLANVSALVFVPLLVSEANNLSPGQAGLVLTPGAVALAIISPMAGRWSDRIGV
                     RPLIFAGLATMLLSVLSISTFGAGAAPLVVSLGMLGIGAGFALANSPNVNAAASSLGP
                     DEVGAGLGIFNGAFFLGGGTGPAVIGAFLAARKEAEAGALNPLYALDTAAFSDAFFAL
                     TLALLISLVAALGLRSTTAKETP"
     gene            3718..4575
                     /locus_tag="VN178_07510"
     CDS             3718..4575
                     /locus_tag="VN178_07510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWS80857.1"
                     /translation="MSVAPLADPPDLAARVAKSADSAGLDLMGIQDHPYQRRFLDTWT
                     LISTLVPVTEHIRFFPDVVNLPLRPPVMLAKAAASLDVLSGGRVEMGLGAGAFWDAVA
                     GMGGPRRSPGEAVRSVEEAIKVMRLVWSDERSLRFDGEFYSLKGMRPGPHPVHDIGIW
                     VGAYGPKMLDLIGRLADGWVPSYGYSPPGRIPEMNRRIDEGAERAGREPREIRRVYNV
                     SGTISAADEGPLEGPPSRWVETLTEFALEQGMDTFVFWPSGNHEQQVEVFATEVVPAV
                     KEAVESGRS"
     gene            4704..4928
                     /locus_tag="VN178_07515"
     CDS             4704..4928
                     /locus_tag="VN178_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80858.1"
                     /translation="MGQKVDLNAARRHGARKAVVGLLSMRSPYSILSFLSAGSILSAG
                     SILSIGSAGSILGLGSAGYSPFREREDQEE"
BASE COUNT          807 a         1605 c         1604 g          944 t
ORIGIN      
        1 caggggacct ggagcaggta ctcagtgggc tcacgcccgg atcggtcgac gactacgtga
       61 gcggtatgga gacccgcgat gatgcggggt tcataccgtt cggcggaggc ctgctcctgc
      121 agacggccga tttcttcaca cccatagttg acgaccctta caggttcggc cagatcgctg
      181 ccgccaacgc tttctcggac gtctacgcga tgggaggaga gcccttgacg gcgatgaatc
      241 tggtcgcctt tccctgctcg ctcgacctcg gggttctgcg tgagatcctg gccgggggcc
      301 tcgccaagat cgaagagtca ggcgccaggc tctgcggtgg tcactcggtc caagacgagg
      361 agccgaagta cggcctctca gtaacgggct tcgtcgagga agaccgggtc gtgcgcaacg
      421 cgggagccag tgccggagac gccctggttc tgaccaaacc gctcggtctc gggatcctga
      481 cgacggcgct caagagagac ctcgtctcgg acgaagagat cgaagacgcc gtacggtcgg
      541 ccaccctcct caacaaggcc gccggcgacg cgatgcgcga ggtcggcgtc tctgcggcca
      601 cagacgtaac gggctacggc ttcctcggcc acctgacgga gatgctgcat gcgagcgaag
      661 tcggagccga cgtgcgcaga gacgaggttc ctgtctggga gcgggccgtg cctctcgcga
      721 acgagggcgt ctaccctggc ggactcaaga gcaacaggga gtacctaaag aaccgtgtta
      781 cggcggacgg tgcaagccag gatgacctgt taccgctcta cgatccgcag acgagcggag
      841 ggttgctcgt cgccgtgcca gggaatcgcg tctccgcgct ggtcggcgag ctcgacaggc
      901 gcggcgtatc gggagccgtc gtcggcgagg tggtcgagca gccgggggtc agggttatca
      961 ggtagccccc agacctcacg gctgcggtct ggtaacgtaa ccgctggccc ggtgaggaag
     1021 cgcctgatcg ttcacagtac ccaaaggtgt actaaagctg accgctgata tctgatagcc
     1081 cgaatgcaat ctatactcaa ctccgcgctg cctttcttcg ccctgatcct ctgcggctac
     1141 gtcgcgggac gcctgaggct cctggacgag acggcagcgg caggggtgaa tacgttcgtc
     1201 ttctacttcg ccctgccgtc cttcctgttc ggcctgatgt cctcctcgcc aataggagag
     1261 gtcgtcaacg taccgtttat cggggcttac gtgacggcga gcgtggtcct cttcgcgctg
     1321 accgcgcttt tgggactcta tctcttcgag ataggacgcg ccgaggcggc ggtgcagggg
     1381 ctcgcggcgg tcctggcgaa cacgggatac atgggtatac ccctggtctc cgccgtcttc
     1441 ggcagggagg ccgccgtccc cgtagtcgtt ggccttaccc tcgacggggt gctgatgatc
     1501 ccgctcggta tcatgatcat cgagtcctcc aaaggccgcg cagaaggccc cgtccgcacg
     1561 gcgctctcca ccttctcctc cctcgcccga aaccccctga tcctctccat cttcgccggc
     1621 ctggccgtct cgaccgcagg actcgacccc ccaacgcccc tggccaactt cctcgatctg
     1681 ctcggcggca cggcaggccc ctgtgccctc ttcgcgctcg gcgcgaccct tgcaggacgc
     1741 accgttgccg gcggaaccgc agagatctcc tatatgacct tcatgaagct cgcgctacac
     1801 cccgcggccc tctttttcac gaccaccgtg atcttcaccg tcgaccccct ctgggctacc
     1861 gcagccctgc tcggcgcggc actcccgatc gccgcgaacg tctttatcgt cgcccgccag
     1921 tacgacgtct acgtcgcccg tacctccagc gcgatcctga tctccacggc ggtctctatg
     1981 atctccgtct ctgcgttatt gttggtcctc caataggcgg gaaatcgtac caaagtccag
     2041 taggaccccc acccttcgtc cggtgtaatg atgacccctc cctcatacga tgtgggggca
     2101 catcaagact tgatatccac ctcgtcggtg gtattgctag tctagccggc caagtagggt
     2161 cgccaagata ggagagcagc atggccagta tcagggagac catcgagcgt gaggtaatgg
     2221 ggtggagacg ggcgcttgca gcgtcctggg ttgccggggc cccggtttga gcgccgatga
     2281 agtagtgcca gggaagcccg aggtctccaa acggctcctg atcggggtcc tggtctcggc
     2341 cgtgttcgtc tccgtgctca acagctcgat ggtcaacgtc gtgatccccg taatagggga
     2401 ggactacggt gtcacggagg cgcaggtcgg gtgggtgatc accggctacc tgctgaccta
     2461 cgccgtgggc atcccgctct acgggcgggc tgcggacgtg ttcggcatcc ggcgcacctt
     2521 catagtaggg ctcctcgctt tcgccgctgg gagcctcctg tgcgcgtggg ctccgagtct
     2581 cgtcgcgctc gtctttgggc ggatcgtaca gggcgttggc ggggcggcta taccggcgct
     2641 ctcgagcgtc tccgtcacga agatactccc tgcgggagag cggggggcgg cgctgggcct
     2701 catcgtctcc agcgtgggca tcggggccgc cataggaccc attctcggcg gcgcggtggg
     2761 agcgctggtg ggctggcact atctgttcgt cgggaccctc attctgactg ccatcctgat
     2821 acccgccgcg ctctacgtcc tacccggcga cagcccggag gaggcgcgcg gcttcgacct
     2881 gcccggcggt atattgctcg gcctcgcggc cggactgttc ctgttcggga tcacccgcgg
     2941 gcaggtctcc ggattcggtt cggcgggctc ctggggcagc tttgtcggct ctgttctggc
     3001 cgccttcggt ttcggctggc gaataaggaa cgccgccgag cccttcgtct cccccacgct
     3061 cttcaggaac agggcgtacg tcgccgccgt ggtcgtcggg tacttttcga tgctcgccaa
     3121 cgtctcggcg ctcgtcttcg taccgctgct cgtctccgag gccaataatc tctcgccggg
     3181 acaggccggg ctcgtgctca cgcctggcgc ggtggcgctc gccataatct cgccgatggc
     3241 cggacggtgg tcggaccgga tcggggttcg tccgctcata ttcgccgggc tggcgaccat
     3301 gctgctctcc gtgctctcca tctccacgtt cggggccggg gccgcgcctc tggtcgtctc
     3361 gctcggcatg ctcggcattg gcgccgggtt cgccctcgcc aactccccca acgtcaacgc
     3421 ggccgcctct tccctaggac ccgacgaggt gggagcgggt ctggggatct tcaacggtgc
     3481 gttcttcctc ggcggcggga cggggccggc cgtgatcggg gcgttcctcg cggcccgcaa
     3541 ggaggctgaa gccggcgcgc taaacccact ctacgcactc gacaccgcag cattctccga
     3601 cgctttcttc gcccttaccc tggccctcct catttcgctg gttgcggcgc tggggttgag
     3661 gagcacgacc gcaaaggaga ctccctgagc gactacggca gagaactcga gttcggcgtg
     3721 agcgtagcgc cgctcgcgga cccacctgat ttggcggcgc gcgtcgcaaa gtccgccgat
     3781 agtgccggac ttgacctgat gggtattcag gaccaccctt accaacgacg ctttctggac
     3841 acctggacgc tcatctcgac gctcgtgccc gtgaccgaac acattcgctt cttccccgac
     3901 gtcgtcaacc tcccgctccg tccgccggtc atgctggcga aagcggccgc cagcctcgac
     3961 gtactctcgg gagggagggt ggagatggga ctcggcgcag gggccttctg ggacgccgtt
     4021 gccggaatgg gcggaccgcg cagaagcccg ggcgaagccg tccgctccgt cgaagaagcc
     4081 ataaaggtca tgcgactggt gtggagcgac gagcgttcgc tgcgcttcga cggcgaattc
     4141 tattccttga agggcatgcg gcctggtccg caccccgtac acgacatagg gatctgggtc
     4201 ggggcctacg ggccgaagat gctcgacctt atagggaggc tggcggacgg ctgggtaccc
     4261 tcctacggct actcgccacc cgggcgaatc cccgagatga accgaagaat cgacgaaggc
     4321 gccgaaagag caggaaggga gccgcgggag atccggcggg tgtacaacgt ctcaggcacc
     4381 atcagcgccg cggatgaagg tcccctcgaa ggtcccccat cgaggtgggt cgagacgctc
     4441 acagagttcg ccctggaaca gggtatggat actttcgtct tctggccatc cgggaaccac
     4501 gagcagcagg tcgaggtctt cgcaaccgag gtagtgcccg ccgtaaagga ggccgttgag
     4561 tcggggcgct cctgatagcg ggattggaaa gggtccttcc caatcccgca ctggttatac
     4621 ccgtgcgtac ggctgaagct ctgcgcacag tgctctcaga cgcggtgata aactgccgcc
     4681 aggcggcgtt ggagggaggt atattggggc agaaagtgga tctgaatgca gcacggcggc
     4741 atggggcgcg caaggccgtg gtcggcctgc tgagcatgag gagcccttac agcatactga
     4801 gctttctgag cgcggggagc atcctcagcg cgggctccat cctgagcatc gggagcgcgg
     4861 gctctattct cggcctgggc agcgccggat acagcccttt ccgagaacga gaagaccagg
     4921 aagaatagca tttcagcatg tcagcgcgct caggctttta
//
LOCUS       DATMVF010000149         6812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_429626, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000149 DATMVF010000000
VERSION     DATMVF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429626"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VN178_07520"
     CDS             complement(<1..901)
                     /locus_tag="VN178_07520"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWS80859.1"
                     /translation="MKKAVVPLLICVLWLGACGQGTGSGQADQSVIRVALGDIESVET
                     LNLFIALEHVRERGVEVEINELADEDLANQAVVSGQADVGLGAPYGLIEGSSAPLRIF
                     CQLQTLRYFPVVDKAAYPDWQALDGEPFTVHSRGSSTEALARLVEQEEGIEFGEISYV
                     SSSEIRANALLRGNVKATMLDIPNKNYVMSEAPGQFHILPLPETGASDEVLFGNTDWL
                     KQNEKTVQILLEETLTVWRAIAEDPSYVMKERERLGLLADLPPEQEEEILPYYRQAAE
                     EGLFTQDCGGREAARDDLEFYKTA"
     gene            complement(1274..1825)
                     /locus_tag="VN178_07525"
     CDS             complement(1274..1825)
                     /locus_tag="VN178_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80860.1"
                     /translation="MKIAVVGRGNVGGGLGDLWEQASHEVKRIGREGGDVSDAEVVVV
                     AVPGGMIAEALKSVQGIDGKTVVDATNLVGADPPEGFSSNAEYVKSRTNGPTAKSFNT
                     NFAALYDRIGEARSTPCNLWCGDEEARQVVEQLNRDAGYEPVYAGPLENAALQENFLK
                     LEFAINQGGLGRFFYRMAPPDRL"
     gene            complement(1944..2420)
                     /locus_tag="VN178_07530"
     CDS             complement(1944..2420)
                     /locus_tag="VN178_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS80861.1"
                     /translation="MEERTAKMSYFPTKILLATDASKDAVKAARIASDMASVSGSELH
                     ILHVGNTKEFHVSPGAEQSFSPRSPSLGEVREKAEKTLEEAVKQVEEAGGAVAGTHLR
                     MGDPDDEILRACEEHGDFGLLVMGSRGLGPIKRRLMGSVSESVVRHARCPVLVARS"
     gene            complement(2613..4916)
                     /locus_tag="VN178_07535"
     CDS             complement(2613..4916)
                     /locus_tag="VN178_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWS80862.1"
                     /translation="MSRSKKMLPVLGQVSQEPEVGGQESARDGADGCTDDCCSSPGMV
                     ETPAATSKTGEENSRAGEGGDAPVVRERTVLRVEGMDCASCAATVEKRVSAMPGVMRA
                     TVNFAAGRLDAEHDPGLSREELEEAVRAAGYGVVETEEAERTPFWRTPRMLLTAASAL
                     LFCVGLVLSLAGGPEIARVVIYLAAIAVGGLPIFRAAVAGLRARHLDMNVLMSAATVG
                     AVGIGEWAEAASVVVLFAAGNALQVYAVDRTRGAVRALVRLTPNEVLVKRGGSEVVVP
                     ADEVGVGDTVVVRPGERLAVDGTVVEGTSAVDEAPVTGESVPVEKGVGDEVYSGSLNG
                     QGGMLVEATRRAGDSTLQRIVRLVEEAQASKAPAEQFVDRFSRVYTPVVVAAAIVLAV
                     VPPLLGGGFGEWFYRALALLIIACPCALVISTPVTVVSGIGAASRRGILVKGGAALEA
                     AGRLEALAFDKTGTLTEGRPVVSRVVPLNGKGEAEVLAVAAALERRSEHPLAHAILAA
                     ADDYGVGGLPTVEGFRSVAGRGAEGEVEGRRYVIGSPRLFAEAGISLGGAETALGEVE
                     REGETPVVIGDKEGPMAVFGLADTLRPDARTTIEALRESGVGELVMLTGDAEAPARRT
                     AEALGVGYRAGLLPENKVEAVRRLVARHGNAGMVGDGVNDAPAMAASTVGFAMGAAGT
                     DVALETADVALMQDDLPKLAEAVRLSRAAERIIRQNVFVSIAIKGLFVLLAPFGLVAL
                     WLAVLADMGTSIAVTLNGLRLFRNGSA"
     gene            complement(4913..5281)
                     /locus_tag="VN178_07540"
     CDS             complement(4913..5281)
                     /locus_tag="VN178_07540"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWS80863.1"
                     /translation="MTNDRCDLLCLDAPRAEDIRKGLLGGEAAYDAAERAKALSDPTR
                     LRLAAALREGGELCVCDLAWISERSQNLVSHHLRTLRSHGLVRSRRDGKLVMYSLTDE
                     GLSLLSAVLGERVGVAGAKA"
     gene            complement(5647..6651)
                     /locus_tag="VN178_07545"
     CDS             complement(5647..6651)
                     /locus_tag="VN178_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWS80864.1"
                     /translation="MSKTVLVVGSMASAMLLASMVAVLTAVPSIGQSQTVTLVGAGDI
                     AECSPDNNAKATAALLNNIPGTVLALGDNAYPHGTPKHYANCYDNYRISDGSVFDAKR
                     THWWGQYKARTMPILGNHDYHSSKIAQPYFDYFSANRAVGFKQPPAPVPNTTANPGLT
                     PGKGYYSYDRGSWHIVALNSNCNKVGGCEKTSTQGKWLRNNLANTNKLCTLAYFHHPL
                     YSTGNNVTNPNVKPLWQILYNHHADVILNGHAHRYERFAPLTPGGKKDPQNGIRQFVA
                     GTGGSLGGSEVQQAPGVEVVETGTSGVIKLDLSARSYSWRFVHIEGQTFTDSGEGSCH
                     "
BASE COUNT         1129 a         2374 c         2000 g         1309 t
ORIGIN      
        1 cggcggtctt atagaattcg aggtcgtccc gggccgcctc acgacccccg cagtcctgcg
       61 tgaacaggcc ctcctcggcg gcctgcctgt agtagggcag tatctcctct tcctgctcgg
      121 gtgggaggtc ggcgagaagc ccgaggcgct cgcgctcctt catcacgtac gaggggtcct
      181 ctgctatcgc acgccatacg gtcagggttt cctcgagcag gatctgcacc gtcttctcgt
      241 tctgcttgag ccaatcggtg ttgccgaaga gcacttcatc gctcgctccg gtctccggca
      301 agggcaggat gtggaactgt cccggcgcct cgctcatcac gtagttcttg ttcgggatgt
      361 cgagcatggt ggccttaacg tttccccgca gcagcgcatt cgcgcgtatc tccgagcttg
      421 acacgtagct gatctcgccg aactcgatgc cctcctcttg ctcgacgagc cgggccagcg
      481 cctcggtgct cgagccccgc gagtgcaccg taaacggctc gccgtcgagg gcctgccagt
      541 cggggtaggc ggccttgtcg accacgggga agtaccgcag ggtctgtagc tggcagaaga
      601 tccggagggg agccgaagag ccttcgatca ggccgtacgg cgcaccgagc cccacgtcag
      661 cctgcccgct gacgacggcc tggtttgcaa ggtcctcgtc ggccagctcg ttgatctcga
      721 cctccacgcc gcgctcacgc acgtgctcca gggcgatgaa caggttgagg gtctccaccg
      781 actcgatgtc gcccaacgcg acccggatga cgctctggtc agcctggcct gagcccgtcc
      841 cttgaccaca cgccccgagc cagagtacgc agatcagtag cggaaccaca gccttcttca
      901 ttgcttgctc cttatcggat gagatccaat cggatcaggt tcgccgccac aggtgctcaa
      961 cgcggagcac gccgcccttg ggtaacgcct tcaaagcgtc caccagcacc atgcgtcgcc
     1021 atcagcttcc cccgaatttc ccctgcggcc tcgaacgtgt gcgagtgctg ccactcaact
     1081 accgaaaccg tcctaattgt cgttcgccag gcaattcttg taagagatgc gatataccca
     1141 attagctgtg tcactaaact gctggaaatc gcctccacga taagtggtcc cggccggccg
     1201 aggtcaacca cgcgacgaaa tatctgtgaa gttcgtaggg atgacctttc gtcttacctc
     1261 ctcgatcgta gcctcagagt cgatcgggag gtgccatccg atagaagaat cggcccagcc
     1321 ctccctggtt gatcgcgaac tcgagcttga ggaagttctc ctgcaacgcg gcgttctcca
     1381 gaggccctgc gtagacaggc tcgtagcctg catcccggtt tagctgctcg acaacctgcc
     1441 gcgcctcctc gtcaccgcac cacaggttgc acggtgtcga gcgcgcttcg ccgatccgat
     1501 cgtagagcgc tgcgaagttg gtgttgaaag acttcgccgt cgggccgttc gtcctggact
     1561 tcacgtattc ggcgttcgag gagaagccct ccgggggatc cgcacccacg aggttcgtgg
     1621 cgtcgacaac cgtcttgcca tcgatgccct gcacgctctt tagtgcctcg gcgatcatgc
     1681 cgccagggac ggctacgacc acgacttcgg cgtccgagac gtcgccgccc tcacgcccga
     1741 tcctcttcac ctcgtggctg gcttgctccc aaaggtcgcc gagcccgcca ccgacgttgc
     1801 ccctgccgac gacagcgatc ttcattttgc tccctcctca cccttccccg cccctgttgt
     1861 ggaactcact tccccaagga gttagcgagc atctgcgatg accagccgag taatacgcga
     1921 cggagtgttc cggcgctgca actctagctg cgcgccacaa ggaccgggca gcgtgcgtgg
     1981 cggacgacgc tctcggagac gctccccatg agcctgcgct tgatggggcc caggccccgg
     2041 ctccccatga caagcaggcc gaagtcaccg tgctcctcgc aggcccggag tatctcgtcg
     2101 tccggatcgc ccatcctcag gtgcgtcccc gcgacggcac ccccggcttc ctctacctgc
     2161 ttcacagcct cctctagcgt cttctcggcc ttctcacgga cctcacccaa ggaagggctg
     2221 cggggggaga aactctgctc cgcacccggg gagacatgga attccttcgt gtttccgacg
     2281 tggaggatat gtagctccga gccgctgacg ctcgccatgt cgctggctat ccgggctgcc
     2341 ttgaccgcgt ccttcgaggc gtcggtcgct agcaggatct tcgtcgggaa gtagctcatc
     2401 tttgccgtcc tttcttccac ctccctcgga atcattctac cagtggtgct accagtggcg
     2461 ccatctctcg aaagactggt cagcatttct ggttgctggc cttcggatca tgccagccct
     2521 tgctggcgaa actagtgcgc acgcggcgtt ttcttgggca cggccgaccc cgcgtatcgt
     2581 gccggtccca ggacactatt tagcccgcgg ctctaggcgc tcccatttcg gaacaaccgc
     2641 aggccattga gggtgacggc tatggacgtt cccatgtcgg cgagcacagc gagccacagc
     2701 gccaccagcc cgaatggggc cagcagcacg aacagtccct tgatcgcgat ggagacgaag
     2761 acgttctgcc ttatgatccg ctcggccgcc cgcgagagcc gcaccgcttc ggccagcttc
     2821 ggaaggtcgt cctgcatgag cgccacgtcg gccgtctcca gcgcgacgtc cgttcctgcc
     2881 gctcccatgg cgaacccgac ggtggaggcg gccatagcgg gggcgtcgtt gacgccgtcg
     2941 ccgaccatgc ccgcattgcc atgcctcgct acgagccgcc gcacggcctc gaccttgttc
     3001 tctggcagga gccccgccct gtacccgacg cccagggctt ccgccgtcct cctcgcaggc
     3061 gcctctgcgt cgccagtgag cattaccaac tcacccaccc ccgactcgcg cagcgcctcg
     3121 atggtggtcc tggcgtcggg acgcagggtg tcggccaacc cgaagacggc catgggacct
     3181 tccttgtcgc caatcacgac cggcgtctcg ccttcccgct cgacctctcc gagggccgtc
     3241 tcggcgccgc ccagagatat accggcctcg gcgaagagcc tgggactccc gataacgtac
     3301 cgtcttcctt cgacctcgcc ctcggcgccc cgtcctgcga cagagcggaa accctcgacc
     3361 gtcgggagtc cgcccacacc gtagtcgtcg gccgcagcga ggatcgcgtg ggccagggga
     3421 tgctccgagc gacgctccag cgcggccgcc accgccagaa cctccgcctc gcccttaccg
     3481 ttcagcggca caacgcgtga gaccacggga cgaccctcgg tgagcgtccc cgtcttgtcg
     3541 aaggcgaggg cctcgagcct ccccgccgct tcgagggcgg cgccgccctt caccagtatg
     3601 ccccgcctgg aggccgcacc gatcccggag actacggtga cgggcgtgga gatcacgagc
     3661 gcgcaaggac acgcgataat gaggagcgcc agcgcccggt agaaccattc gccgaagcca
     3721 ccgccgagca ggggcggcac gaccgcgagg acgatcgccg ctgccaccac cacgggcgtg
     3781 tagacccgcg agaaacggtc cacgaactgc tcggcgggag ccttactggc ctgggcctcc
     3841 tccaccagac gcactatcct ctgcagcgtc gagtccccgg cccgcctggt cgcctcgacg
     3901 agcatcccgc cctggccgtt cagggagccg gagtacactt cgtctcccac ccccttctcc
     3961 acgggcacgc tctcacccgt caccggggcc tcgtccaccg ccgaagtgcc ctcgacgacg
     4021 gtgccgtcca ccgccagcct ctcgccgggc ctgacgacca ccgtatcgcc gactccgacc
     4081 tcgtcagcgg gaaccacgac ctcggagccg cccctcttga cgagcacctc gttcggggtc
     4141 aggcgcacga gcgcccgcac ggccccccgc gtgcggtcta cggcgtacac ctgcagggcg
     4201 ttgccggcgg cgaagaggac cacgaccgag gcggcctccg cccactcccc gatccccacc
     4261 gcgcccaccg tggccgcgct catcaagacg ttcatgtcca ggtgcctggc ccttagcccc
     4321 gctacggccg cccggaagat cggcagcccg ccgacggcga tggcggcgag gtagattaca
     4381 acccgcgcga tctctggtcc tccggccagg gataatacga gtcccacgca aaaaagcagc
     4441 gcagaggccg ccgtcagcag catccgcggt gtgcgccaga agggcgtcct ctcggcctcc
     4501 tcggtctcga ccaccccgta gccggcggcc ctcaccgcct cctctagttc ttctcgcgag
     4561 agccccgggt cgtgctcggc gtccaggcgt ccggcggcga agttcacggt cgccctcatc
     4621 acgccgggca tcgccgagac gcgcttctcc acggtggccg cgcaactcgc acagtccatc
     4681 ccctctacgc gcaagacggt cctctcccgt acgacgggcg catcgcctcc ctcgcccgcc
     4741 ctggagttct cctctcctgt cttcgaggta gccgcaggcg tctctaccat cccggggctc
     4801 gagcagcagt cgtcggtgca gccgtcggcc ccgtcgcgtg ccgattcctg tcccccgacc
     4861 tcaggctcct gcgacacctg ccccaagacc ggaagcatct tcttagagcg gctcacgcct
     4921 tcgctcctgc cacacccacc ctttcgccga ggacggcgga gagtagcgag agaccctcat
     4981 cggtgaggga gtacatcacg agcttgccgt cgcgccgcga gcggacgagc ccgtgcgaac
     5041 gcagcgtcct caagtggtgc gaaacgaggt tctgggagcg ctcggagatc caggccaggt
     5101 cgcacacgca tagctcccca ccctccctca aggccgcagc cagcctcaga cgggtcgggt
     5161 ccgagagcgc cttggccctc tcggcagcgt cgtacgccgc ttccccgcca agtagcccct
     5221 tccgaatgtc ctccgcgcgc ggagcgtcca aacagagcag gtcacagcgg tcgttagtca
     5281 tcctcacact ccaatagccg ttgatacgtt gggttgagca tagcaggtct gaccgcgatt
     5341 ggctatgctc cattaaggta gcccacgctc accgcgcata tcacgcccgc ggtcggttgg
     5401 attggcgcgg cttcattcgg cattggatag atcggtctga tatgctcttc tcatgttcgg
     5461 tatcctaccg agcgagcgct aaagagcccc ttttcggaca ttttcttcca cgcactccgg
     5521 tgcataagcc cgtcctctgg agttgggcta tgaaacggac tggggcctcc gccccggtcc
     5581 gcgctgttac tgagtctgcc ccacgtcctc tcccgacggc ctctctcgtc taagttgctg
     5641 cacaccctag tggcaagagc cttcgcccga gtcggtgaag gtctggcctt ctatgtggac
     5701 aaacctccag ctgtaagagc gggcgctgag gtcaagcttg ataactcccg acgtgccagt
     5761 ctccacaacc tccactccag gggcctgctg tacttcactg cctcccagcg agccgccggt
     5821 cccggccacg aactgacgga tgccgttctg cggatctttc ttgccccctg gagttagtgg
     5881 ggcgaagcgc tcgtagcggt gggcgtgccc attgaggatt acgtcggcgt gatggttgta
     5941 gaggatctgc cagaggggct tcacattagg gttggtgaca ttgttgccgg tggaatagag
     6001 tgggtgatgg aagtaggcca gggtgcacag tttgttggtg ttagcaaggt tgttgcgaag
     6061 ccatttgccc tgggtagatg tcttttcgca tccgcccacc ttgttgcagt tgctgtttag
     6121 ggccacgatg tgccaggatc ccctatcgta agagtagtag cctttgccgg gtgtcaagcc
     6181 agggtttgcg gtcgtgttag gcaccggcgc cggaggctgc ttgaagccaa cggcccggtt
     6241 cgcgctgaag tagtcgaagt agggctgcgc tattttggaa gagtggtaat catggttgcc
     6301 caaaattggc atcgtgcgag ctttgtactg accccaccag tgggtgcgct tggcatcgaa
     6361 gacactacca tcggagattc tgtaattatc gtaacagttg gcatagtgct ttggggtgcc
     6421 gtgggggtag gcgttgtctc ctagagcgag gacggtcccc ggtatgttgt tcagcagagc
     6481 cgctgttgcc ttggcgttgt tgtctgggga gcactcggcg atgtctcccg ctccgaccag
     6541 cgtcaccgtc tgcgactgcc ctatgctcgg cactgccgtc agcacggcca ccatggatgc
     6601 cagcagcatc gccgaggcca tcgatccgac aacaagaacg gtcttgctca tccccttcat
     6661 cctttgtaga aagcaacttt ccccgacgtt cgatagataa ggggagagta ggagccccgg
     6721 ctctctcaca tatctagctt tcgaattctg cgtctcgcta cttcgccttg tatgcgacga
     6781 cctcaccggc cgtgacttgg ggtgggttcg gg
//
LOCUS       DATMVF010000150         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_568184, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000150 DATMVF010000000
VERSION     DATMVF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568184"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /gene="trpD"
                     /locus_tag="VN178_07550"
     CDS             <1..446
                     /gene="trpD"
                     /locus_tag="VN178_07550"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWS80865.1"
                     /translation="GAPYVRPVAEALRDLGAEKALVVHGTDGMDEITVTDKTLVADVS
                     EGEIVEYEISPEDFGLPRHSPDGLLGGDAHLNARVLRDILTGEERGASRDIVLLNAGA
                     AIHVSGRARSIEEGVRLARDAISDGAAATALQDFIKTTRRLSGTV"
     gene            443..1210
                     /locus_tag="VN178_07555"
     CDS             443..1210
                     /locus_tag="VN178_07555"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWS80866.1"
                     /translation="MKRATVLDELATAAIERAQDLRSRSDLGTLYQEALLFEKRDFTG
                     ALSGPGLAVISEIKRASPSAGFIRAADPAEWGARYELEGAGCLSVLTEPERFQGSLED
                     LDAARKVTTLPVLRKDFTVDEAQILETGTRADAVLLIAALFEAAALARYVSLAVEVGL
                     TPLVEIHDEEEANLALESGARVIGVNNRDLRDFTVDLGTFERLAPKLAGATLIAESGI
                     KTPEDARRLRDAGADAVLVGEAAMRDPTLVARLASLP"
     gene            complement(1499..2245)
                     /locus_tag="VN178_07560"
     CDS             complement(1499..2245)
                     /locus_tag="VN178_07560"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWS80867.1"
                     /translation="MRRISPRPILLALTLMVVIGSIVAIELRLDARGADRGGTQASAG
                     KQPKSGPQVARNAEDRPGLSGNAGRSDSSERDKDENPESSVSDEERIAIKEDEFQRAR
                     EITGSTGFINTDGVSIKDAVGEKVVLVEFWTYSCFNCQNAQPHINGLYEEYEDDGLLV
                     VGVHTPEFGFEKDISNVREAVRQANIQYPVVLDNHYAIWNAYGQRFWPAWYLVDADGF
                     IRFRHFGEGAYGETEQKVQELLAERDRLYD"
     gene            complement(2242..2943)
                     /locus_tag="VN178_07565"
     CDS             complement(2242..2943)
                     /locus_tag="VN178_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HWS80868.1"
                     /translation="MFEPTLVLIAFAGLASFLSPCMLPVIPAFLAQLAGTSLDLADLE
                     RRDVFLGTIMFVAGFSVIFATLGVVLGAVLADVATEALAWLSRLAGTIVIVLGLHLTG
                     LLRLPILDRQYSALPGGLKPGNLASFLFGASFAVAWTPCVGPILGSTLALATTRPATA
                     FPLLLAYAVGLGAPFLLVGLFPSRILSFLKLHRRAAARIHIAFGVVLIGMGVLVFTNQ
                     LNVLANFPLLNEVLI"
     gene            complement(3182..>3710)
                     /locus_tag="VN178_07570"
     CDS             complement(3182..>3710)
                     /locus_tag="VN178_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWS80869.1"
                     /translation="LVEDLFELARIDAGMLQLTLEEASLHDLISDTISSFQPQADQRG
                     VHLVGEVVGDVDPVLANPSRLQRVLHNLVSNALRHTPADGTVALRATPEGEVVRVEVA
                     DTGEGIASKDLPHVFERSFHGEQSLTSTEKDNAPGAGLGLAIARGLVEAHGGTIDVES
                     DPGRGSCFYFTLKRA"
BASE COUNT          740 a         1179 c         1139 g          652 t
ORIGIN      
        1 tcggcgcgcc ctacgtgcgt cccgtcgccg aggcgctgcg ggacctcggg gccgagaaag
       61 cgctcgtcgt ccacgggacg gacgggatgg atgagatcac ggtgaccgat aagaccctcg
      121 tcgcagacgt ctccgaagga gagatcgtag agtacgagat ctcaccggaa gacttcggcc
      181 tcccgcgcca ctccccggac ggcctgctcg gcggcgacgc ccacctcaac gcccgcgtcc
      241 tccgcgacat actcaccggc gaggaacgtg gggcttcgcg cgacatagtc ctgctcaacg
      301 cgggagcggc gatccacgtc tcgggtagag cgaggtcgat agaagaaggc gtgcgtctgg
      361 cgagggatgc cataagtgac ggcgccgccg cgacggccct gcaagacttc ataaagacaa
      421 cccgccgcct ctcggggacc gtgtgaagcg cgcaaccgtc ctcgacgagc tcgccacggc
      481 cgccatcgaa agggcgcagg atctacgcag cagatccgac ctcggcaccc tctaccagga
      541 ggcgctgctc ttcgagaaga gagacttcac cggcgccctc tcagggccag gcctcgccgt
      601 gatctccgag ataaaacgtg cttcaccctc cgcaggcttt atccgcgccg ccgaccctgc
      661 cgagtggggc gcccgctacg agctggaggg ggcgggttgc ctctccgtcc tcaccgagcc
      721 cgaacgcttc cagggaagtc tcgaagacct cgacgccgcg cggaaagtta ctactctgcc
      781 ggtcttacgc aaggacttca ccgtcgacga ggcgcagatc ctcgaaacgg gcaccagggc
      841 cgacgccgtc ctcttgatag cggcgctctt cgaagcggcg gcgctcgccc gctacgtctc
      901 ccttgccgta gaggtcggcc tgacgccgct cgtagagatc cacgacgaag aagaagcgaa
      961 cctggccctc gaatcaggcg ccagggtcat cggcgtcaac aaccgggatc taagggactt
     1021 caccgtagac ctcggcacct tcgaacgcct cgcgccgaaa ctcgccggtg cgaccctcat
     1081 cgccgagagc ggcatcaaga cccccgaaga tgccagaagg ctacgagacg caggcgccga
     1141 cgcggtcctg gtaggcgaag cggcgatgcg agatcccacc ctcgtcgcac gcctggcgtc
     1201 cctgccgtaa gggcggttcg tgtaaatgcg gttcgcgaac cgcctctact gcgggttcct
     1261 tgggacaaac gaccaatgtc atgcacgtca ccctggcgca acgcagaccg ttgggacgtt
     1321 tgatcggcgc gttcaaaacc gtgtcaacgc aacgcgtcaa cgacatcaga gatacccgcg
     1381 acgcctcctt gtggcaacgt gactattacg aacacgtgat tcgcaacggt gcgtaattgc
     1441 gtgcactacg tcattacatc atcaccaatc cctcgctgca tgggccggcg ggccccattc
     1501 aatcgtagag gcgatctctc tccgcaagca gctcctgcac cttctgctcg gtctcgccgt
     1561 aggcgccttc gccgaagtgc ctgaagcgga tgaagccgtc ggcgtcgacc agataccatg
     1621 caggccagaa tcgttgaccg taagcgttcc agatagcgta gtggttgtcc agaacaacag
     1681 ggtattgaat gttcgcctgc cttacggctt cccgcacgtt ggagatgtcc ttctcgaacc
     1741 cgaactccgg cgtatggacc ccgaccacga gcaggccgtc atcctcgtat tcctcgtaca
     1801 agccgttgat gtgcggctgg gcgttctggc aattgaaaca gctatacgtc cagaactcga
     1861 ccagcactac cttctcgcca acagcatcct ttattgagac gccgtccgtg ttgataaagc
     1921 cggtcgagcc tgtgatctcc ctggccctct gaaactcatc ctccttgatg gcgatccgct
     1981 cctcatcgga gacggaactc tccggattct catctttgtc acgttcggag gagtcggatc
     2041 tcccggcgtt gcctgagagg cccggcctgt cctcagcatt cctggcgact tgtggaccgc
     2101 ttttcggctg cttgcccgcg ctcgcctgtg tgccgcccct gtctgcacct cgtgcgtcga
     2161 gccggagctc gatagcgacg atcgagccga tgactaccat cagcgtcagc gccagcagga
     2221 tcggccgggg agagatccgt ctcatatcag tacctcgttc agtagcggaa agttggcgag
     2281 cacgttgagt tggttggtaa acaccaggac acccatcccg atcaggacga ccccgaaggc
     2341 gatgtggatg cgtgccgcgg ccctccgatg cagcttgaga aacgagagga tccgcgaagg
     2401 gaaaagcccg acgagcaaga agggcgctcc gagccccact gcgtaagcga gcagcaatgg
     2461 aaatgccgta gccgggcggg tcgtggcgag ggctagtgtg ctgcccagga tcggtccgac
     2521 gcaaggcgtc caggcgacag cgaaagacgc accgaacagg aaggaggcga gatttcccgg
     2581 cttgagtcct cctgggagtg ccgagtactg gcggtccagt atcggcagac gcagcagacc
     2641 agtcaggtgt agaccgagca cgataacgat cgtgcctgca agcctcgaga gccacgctag
     2701 cgcctccgtc gcaacatccg ccaggaccgc gccgagcacc accccgagtg tcgcgaagat
     2761 gactgagaaa cctgctacga acatgatcgt gcccagaaag acatcccttc tctcgagatc
     2821 agcgagatcg agcgaggtgc ccgcgagctg ggcaaggaag gcgggtatca cgggcaacat
     2881 gcacggcgag agaaaggaag caaggcccgc gaaggcaatc agaacgagcg tcggttcgaa
     2941 catctctacg cttccaagtc caatgttccc ttatacgtgt ttatcggaag aaacgctcct
     3001 gctggcccga cctcgattct cacacgggca cacagggtgg acaatcgatc ccagccgtat
     3061 cgtaagaaac tcttaagggt tcctcacgca atccagtggg cctcctatgg ttggaggctc
     3121 ggaacgtgag acgcgccgta ttgggcggtt cacgaaccgc ccaatacggc ctaccgaggt
     3181 atcaggcccg cttcagggtg aaatagaaac atgatccgcg accggggtcg ctctcgacgt
     3241 ctatggtgcc gccgtgcgct tcgacgaggc cgcgcgcgat cgcgagtccc agcccggcgc
     3301 cgggcgcgtt atccttctcg gtactggtca gtgactgttc gccgtggaac gagcgttcga
     3361 agacgtgagg caggtctttg gaggctatgc cctcgccggt gtcggctacc tcgacgcgca
     3421 ccacttcccc ttcgggcgtt gcgcgcagtg ccacggtgcc gtctgcaggg gtgtggcgga
     3481 gggcgttgga gacaaggttg tggagcacgc gttggagcct ggacgggttc gcgagcacag
     3541 gatctacgtc gcctacgacc tctccaacca ggtggacgcc gcgttgatcg gcctgcggct
     3601 ggaagctgga gatggtgtcg gagatcaggt cgtgcagcga ggcttcttcc agggttagtt
     3661 gtagcatccc tgcgtctatg cgggcgagct cgaagaggtc ctccacgagg
//
LOCUS       DATMVF010000151        10175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_612720, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000151 DATMVF010000000
VERSION     DATMVF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612720"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..786
                     /locus_tag="VN178_07575"
     CDS             364..786
                     /locus_tag="VN178_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HWS80870.1"
                     /translation="MIRMLDHTADVGFELEAPALEALFEEARRALLMTLFERPPEGGE
                     DEGSVRLSAPDLETLLVRWINELAFLVQDDGFVPVHADVRIEETGGEDFLLDANLGGI
                     PLDLEGHGWQGEIKSATFHGLEVTNRSGDWRARVILDV"
     gene            817..2274
                     /locus_tag="VN178_07580"
     CDS             817..2274
                     /locus_tag="VN178_07580"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWS80871.1"
                     /translation="MNIEHVSGFKYRIPKEGKMRVDAVFYASEGILEDLQDEDYASLR
                     QLCNVATLPGVVEPALTMPDIHWGYGFPIGGVAAFDPNDGGVVSPGGVGFDINCGVRL
                     LVSRLGRKDVDSSEGRRVDDPRKNRLADALYESVPSGMGRGRKDTGFSREDLEEVLAR
                     GAGALVGRGYGEPEDLENIESKGRLPASDPEGTSDRAYQRGVSQLGTLGSGNHFLEVQ
                     YVDEIYDEEAARAYGLELGMVTVLIHSGSRGLGHQVCQDYVERCMDAAKMYGIELEDR
                     QLAAAPIRSPEGEAYLGAMSAAANFAFANRQLITHSTRQAFASAGFDSEHQHLRVLYD
                     VAHNNAKFEKHDDREVLVHRKGATRAFGPGNSELPERYRSVGQPVLVPGDMGRYSFVL
                     AGTEGAMSETFGSSAHGAGRKMGRKKAKRAAKGRNIRRELEDKGILIRAANRATVDEE
                     MSEAYKDATEVVDTTDGAGIGKKVARLRPLIVVKG"
     gene            complement(2301..3242)
                     /locus_tag="VN178_07585"
     CDS             complement(2301..3242)
                     /locus_tag="VN178_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWS80872.1"
                     /translation="MDLRINSKSHVPVHVQLEGQIKHLILTGGFEVGSRLPSIRVMAG
                     FLRVNRNTVARVFSDLEREGYVESRRGSGVYVIEPPVEAEDLRRQEVLERVMDLAAAQ
                     GVPVDDLAYALLARAGAKPQEKTPILFVECTRAELDQFSSELEEQLPVEVERVLLEDL
                     ADRVSDAEDLPWRLAVTTFFHIHEVQDIVEPHGIEAVALLAEATLESLQRLMELPSGT
                     PVGVAGLGRTCMENLSRSIEGAGLDHLDFVQVYIDEEPEEVLATLGGVESVVCASITA
                     RRLRELGIPEDLEIIEEDRILDQGGIEMLGRMLRQQP"
     gene            3337..3915
                     /locus_tag="VN178_07590"
     CDS             3337..3915
                     /locus_tag="VN178_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80873.1"
                     /translation="MNYPFDLELARGRQAELLREAEERRIAGALRKARRSVEEGGMTR
                     EGLKVGWGRAEEESAVADLLELNGIPRWVAFEERFIVAQKDGKVLGAVRYRTESKRLI
                     LGLLVVDPWAGEERLALALYRGSRELAREIGAGEVIASESRAGYPGRAGYRRWGRGWR
                     LDLRRHSGAGVRQRLLGVFGALSIPHYRAFRS"
     gene            3921..4637
                     /locus_tag="VN178_07595"
     CDS             3921..4637
                     /locus_tag="VN178_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWS80874.1"
                     /translation="MPEENTSGKEGRITFTLAGALAGARRCVPIAISGCGIGLVFGTL
                     AGQAGLGAAEAALMSAFVFSGAAQFVVLGLWAAPLPVATIVLATLVVGLRHVLMGAAL
                     APVFSKLPRLRAYGSVFFMADENWALTMGEFAKGRRDAAFLVGGGLLMFLAWTGSTFA
                     GGALGGVVEDPSRWGLDFAFTAVFLALIAGMWNGKSDILPWTVAAAVAVAAHHLLPGQ
                     WYILLGALAGSFAGVVRRGH"
     gene            4627..4941
                     /locus_tag="VN178_07600"
     CDS             4627..4941
                     /locus_tag="VN178_07600"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HWS80875.1"
                     /translation="MAIDPGTLLAIILMALATYATRSGGLWLASRFDLSERAGAWLDQ
                     IPGAILVSLVAPAIFMGGPIEVLAAVVVVIVSLRTGSLPAAMVIGVGTVLALRALQTN
                     LL"
     gene            5037..6524
                     /locus_tag="VN178_07605"
     CDS             5037..6524
                     /locus_tag="VN178_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HWS80876.1"
                     /translation="MLKEFEHRYRAVRGRDVRFDGWFYVAVTSTGIYCRPSCPAATPK
                     RSNVRFYPTAAAAQYAGFRACKRCRPDAVPGSPEWDNRADIVARAMRLIADGVVDRDG
                     VAGLARRLGYTERHLHRLLCADVGAGPLALARARRAHTARLLLETTDLPITEVAFAAG
                     FTSVRQFNDTIREVFAVTPRELRRVRGQHDETVRGEIPLRLPFRKPFDAGGMLQFLGT
                     RAVAGVETFDGETYRRTLRLPHGAGVVALSDGGDHVRCVLTLENVRDLGSAVQRCRSL
                     LDLDADPVAVADVLGADPFIGALVRRTPGRRVPGSVDGAELAFRAVLGQQVSVAGART
                     LAGRLVARCGEPLPETLAVGDGEPTHLFPGPHAVAAATLDGLGIPGARRETLRTLARA
                     IAGGGILLDAGSEREDVERRLLEVRGIGPWTASYVAMRALRDPDAFLPSDLGVRKAIS
                     RLGHAGDRQSVVAERWRPWRAYATQHLWASLGDEKDRTAKGEVVA"
     gene            6521..7039
                     /locus_tag="VN178_07610"
     CDS             6521..7039
                     /locus_tag="VN178_07610"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWS80877.1"
                     /translation="MKVYIDEVESPAGPLAFAVNEDGTLVRLHFVESDYGPVMEEDLQ
                     DEGYVVEVDETRTDGVRKELAEYVSGRRKTFDVPLALAGSEWQKAVWTELTRIPFGET
                     RSYGEIADSLGRPGAARAVGRANASNVLPVVVPCHRVIAADGTLGGFNGGLHLKERLL
                     EHERSVLAAAGS"
     gene            7097..7984
                     /locus_tag="VN178_07615"
     CDS             7097..7984
                     /locus_tag="VN178_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS80878.1"
                     /translation="MRPRDFAGLMLLGALWGGSFLFIRVAVPALGPFVLVELRAGLAV
                     VALFLYALAVRSMPKIRGRWRSFLVLGFFNAALPFSLISAAEIHLTASLAAILNATTV
                     MFSAIVAAVWMGDVLTTRKAVGIALGIVGVSVLVGWDPLPLNGAVLLAVAAMLLASLS
                     YALGATYAKRSFAAIPPLGMAIGQLGGATALLLPLAVVSLPERAPSTGVTLSMLGLAF
                     LSTAVAYLIYFRLIENVGPTSTLTVTLLVPVFGLLFGVLLLEEPFGLGTLAGLGIILT
                     SVVLITGIAPRKVGAVQRG"
     gene            complement(7972..8382)
                     /locus_tag="VN178_07620"
     CDS             complement(7972..8382)
                     /locus_tag="VN178_07620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWS80879.1"
                     /translation="MPTLDELNERGMGTLPGLIGLTILEAEEGKISSRLDLRNELMAP
                     NGYLHAATVVALADTSCGYGTFVNLPDGAQSFTTIELKSNFVGTKRGGAIGCEAKLVH
                     GGRTTQVWDATVFDEEGGKPIALFRCTQMILYPR"
     gene            8478..>10175
                     /locus_tag="VN178_07625"
     CDS             8478..>10175
                     /locus_tag="VN178_07625"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWS80880.1"
                     /translation="MSGGTKTRSLRAPRRVRAYLAANAGRPALLLAASEEAAERYARE
                     AEVFTDGPVVHLPSRGAPYGDVFDPAVSRVGRRQRALRSLRSARVVVAGPLAFMERTP
                     LYEPMELDAGAEIELDATLERLVVLGYERVDRISRPGEFAVRGGIVDVFPSTRRSPVR
                     VEWWGDEIESVRAVSLATQRVVRELEAVTVYAAREGDLAGLAATDGNLPEEARRGVRV
                     PGLDRLLLGLNPVSPRTMLPEGVEIWREEPQEDVSEVVEGLVHELYDRGMPDPDVEFS
                     LGEEGETISAPPVAAFADTLREAARRLDALVEDGLAVFVACSSRGEARRTVYAFREIH
                     RTMRETARVDAQLPPGLYALPGEVEEGFTYPEGGVAVFRRDSLLGRRREARRRTGGRS
                     LASFADLKVGDLVVHAMQGIGRFEGLVAKEVLGSTRDYMQVSYRGGDTLFVPYEQMEL
                     LHKYVGGDGARLDKLGGATWAQVTDRVRKRVKALAGELLRLHAARASAPGFAFPEDGE
                     WERELEESFPYQETPDQESAITAVKSDMQRPHPMDRLVCGDVGFGKTEVSVRAAFKAA
                     "
BASE COUNT         1702 a         3220 c         3410 g         1843 t
ORIGIN      
        1 gtgggccggc tggtcgtgat accggacggg ccccacactc tcgtccataa ccggcccctg
       61 gatctggccc gcgtggtccg cgagttcctg agcgccgctc cgctacagag cttcgacgtt
      121 tgaagccatc cggctacgtg gttggcttcg attcctccgc ggcaatgctg cgggttcggt
      181 cgggagctaa cgtggcgacg ttagcgatcg tctagcctcg ggtaccaaca gatagggcga
      241 gatgagtgaa gacgcgcaga tgagtaggcg cttagcagga tgtttgggcg agcaacacgg
      301 ctcatggctg catgcgagga tgctacggag gcaggctctc aaaccactcc aacccgagac
      361 gcgatgatcc ggatgctgga tcacacggct gatgtcggct tcgaactgga ggccccggcg
      421 ctcgaggccc tgttcgagga ggcccgtcgg gccctgctca tgacgctctt cgagcggccg
      481 ccggagggcg gcgaggatga gggctcggtg cgactctcgg cccccgatct ggagaccctc
      541 ctggttcggt ggataaacga actcgccttc cttgtacagg acgacggttt cgttccggtc
      601 cacgcggacg ttcggatcga agagaccggt ggagaggact tcttactcga tgcgaacctg
      661 ggcggaatcc cgctcgacct cgaaggccac ggctggcagg gggagataaa gagcgcaaca
      721 ttccacggac tcgaagtgac taacaggagt ggtgactggc gggcccgggt gatcctggac
      781 gtatgacgat cgtcccacca tagaaggagc agggcagtga acatcgagca cgtctccggg
      841 ttcaagtacc gcatccccaa ggagggaaag atgcgggtgg acgctgtgtt ctacgcctcc
      901 gagggcatcc tcgaggacct ccaggacgaa gactacgcct cgctgcgaca gctctgcaac
      961 gtggcgacgc tgccgggggt ggtcgaaccg gccctgacca tgcccgacat ccattggggc
     1021 tacggtttcc ccataggcgg ggtcgcagcc ttcgacccta acgatggtgg cgtggtcagc
     1081 cccggcggcg tcggcttcga tatcaactgc ggggtgcggc tcctggtctc caggctgggc
     1141 cgcaaggacg tagattctag cgagggccgc agggtcgacg atcctcggaa gaatcgcctg
     1201 gcagacgcgc tctacgagag cgtcccgtca gggatgggaa ggggccgcaa agataccggc
     1261 tttagcaggg aagacctcga ggaagtcctg gcccgggggg ccggcgccct cgtcgggcgc
     1321 ggctacggag agccggagga tctcgagaac atagagtcga aaggacggtt gcccgcctca
     1381 gatccggaag ggacttccga ccgggcttac cagcgcggcg tctcccaact cggaactcta
     1441 ggctcgggca accacttcct cgaggtgcag tacgtggacg agatctacga cgaggaggcg
     1501 gcccgcgcct acgggctcga actggggatg gttaccgtcc tgatccactc cggatcgagg
     1561 ggcctcggac accaggtctg ccaggattac gtcgagcgct gcatggacgc agccaagatg
     1621 tacggtatag agttggagga caggcagctc gccgcggctc ccataagaag tcccgaagga
     1681 gaagcttatc ttggggccat gtcagcggcg gccaacttcg ccttcgccaa ccgccagctc
     1741 atcacccact ccacccggca ggccttcgcg tcggcgggat tcgactcgga gcatcagcac
     1801 ttgagggtgc tctacgacgt cgcgcacaat aacgccaagt tcgaaaagca tgacgatcgc
     1861 gaggtcctgg tacaccgcaa gggcgccacc cgcgcattcg gccccggcaa ctcggaactg
     1921 cccgaacgct accgctcggt aggacagccg gttctggtac cgggagacat ggggcgctac
     1981 tccttcgtgc tggccggcac cgaaggggcc atgagcgaga ccttcggctc cagcgcccac
     2041 ggggcgggac gcaagatggg ccgcaaaaag gcgaagaggg cggcgaaggg ccgcaacatc
     2101 aggcgagagc tagaggacaa agggatcctt atccgcgccg ccaaccgggc caccgtagac
     2161 gaggagatgt ccgaagcata taaggacgca accgaggtcg tggacaccac tgacggggca
     2221 ggcataggga agaaggtggc acgcctcagg cccctgatcg tggtcaaggg ttgaagatgg
     2281 tctcatggca gggatagcca tcacggctgc tggcggagca tgcggccgag catctcgatg
     2341 ccgccctgat ccaggattcg gtcttcttcg atgatctcca gatcttccgg gataccgagt
     2401 tcgcgaagcc gcctggccgt gatgctggcg cacaccacac tctcgactcc gccgagggtg
     2461 gcaagcactt cttcgggctc ctcgtctatg tagacctgga cgaagtccag atggtcgagg
     2521 cccgcgcctt cgatggaacg cgagagattc tccatgcacg tgcggcccaa gcccgcgacc
     2581 ccgaccggag tccccgacgg aagctccatc aacctctgca ggctctccag cgtcgcctcc
     2641 gccagtagcg cgaccgcctc tatcccgtgc ggctccacta tgtcctgcac ctcgtggatg
     2701 tgaaagaacg tcgttaccgc gagtcgccac ggcaggtctt cggcgtccga gaccctgtct
     2761 gcgaggtcct cgagtaagac cctctctacc tcgacaggga gctgttcctc tagctcggag
     2821 gagaactgat ccagctccgc ccgcgtgcac tccacgaaga ggatcggcgt cttctcctgc
     2881 ggcttcgcgc cggcccgcgc gagtaacgcg tacgccagat catcgaccgg cacgccctgc
     2941 gccgccgcga gatccataac cctctccaaa acctcctgac gcctcaagtc ctccgcctcc
     3001 acaggaggct cgatcacgta cacgccactg ccgcggcggc tctccacgta tccctcccgc
     3061 tccaggtcgg agaacacccg cgccaccgtg ttcctgttca cccgtagaaa tccagccatc
     3121 accctgatgc tcggcaaccg gctcccaacc tcgaagccgc ccgtcaggat gaggtgcttg
     3181 atctgcccct ccaattgtac gtgtacgggt acgtgactct tcgaatttat ccgcagatcc
     3241 ataaattaac ctattgtcct atgacactta gccagttata attgtcccac atcagcgtga
     3301 caattatagc gcaatcggcg agcatggagg tgagttgtga actatccgtt cgatctggag
     3361 ttggcaagag gacggcaggc agagctatta cgcgaagcgg aggagcgtag gatcgcgggt
     3421 gctttgcgta aggctcgtcg aagcgtcgag gagggcggta tgaccaggga gggtttgaag
     3481 gtgggctggg ggcgcgccga ggaggagtcc gcggtcgcgg atcttctgga actgaatggc
     3541 ataccgcgat gggtggcctt cgaggagagg ttcatcgtcg cgcagaagga cggaaaggtg
     3601 cttggcgcgg tgcgttaccg gacggagtcc aagcggctaa tacttgggtt actcgttgtg
     3661 gacccgtggg cgggcgagga acggctggcc ctggcgttgt accggggatc aagagagctg
     3721 gcgcgtgaga taggtgctgg agaggtcatc gcatcggagt cgcgggcggg ttatcctggc
     3781 cgggccggat accggcggtg gggtcgcggc tggcggcttg atctgagacg ccactctggg
     3841 gcgggtgtca ggcagcgatt gctaggggtg ttcggcgcac tctccattcc acactatcgg
     3901 gcgttccgca gttaggcgtc gtgccggagg aaaacacctc agggaaagag ggccggatca
     3961 cgttcaccct tgcgggcgcg ctcgcggggg cgagacgatg cgtgcccatc gctatcagcg
     4021 gttgcggcat cggcctcgtg ttcgggactc tggcgggtca ggccggtctc ggcgccgctg
     4081 aggccgcgct gatgagcgcg ttcgtattct cgggggcggc acagttcgtt gtgcttgggc
     4141 tatgggcggc cccgctgccg gtagctacca tcgtcctcgc tacgctcgtc gttggtctca
     4201 ggcacgtgct gatgggcgcg gccctcgcgc ccgtgttctc gaagttgccg cgtctcaggg
     4261 cttatgggtc cgtgttcttc atggctgatg agaactgggc gctaaccatg ggcgagttcg
     4321 ccaagggacg ccgcgacgcc gcgttcctgg tcggcggcgg gttgttgatg ttcctcgcct
     4381 ggacagggtc cacattcgcc ggcggcgctc ttggcggtgt ggtggaagat ccctcacgct
     4441 gggggctcga cttcgctttc accgccgtgt tcctcgcgct catcgcgggg atgtggaacg
     4501 ggaagtccga catcctcccc tggacggtcg ctgctgcggt cgctgtggcg gcgcaccact
     4561 tgctgccggg ccagtggtac atcctgctcg gcgctctcgc agggagcttc gcgggggtgg
     4621 tgcggcgtgg ccattgatcc cggcaccctg ctcgcgatca tcttgatggc gctcgcgacg
     4681 tacgccaccc gctcgggtgg gctctggctc gcgagcaggt tcgacctctc cgagcgagca
     4741 ggggcgtggt tagatcagat accgggtgcg atcctcgtct cgctcgtcgc ccctgccatc
     4801 tttatgggcg gaccaatcga agtgttggcc gcggtcgtcg tcgtgatcgt ctctctcaga
     4861 acagggagcc tgcccgctgc gatggtgata ggggtaggaa ccgttctcgc gctgcgagcc
     4921 ctgcagacga acctgctatg accccccacg ctcctcatct ccacagtacc gactccgatg
     4981 ataccgatgt ccgatttccg ccagtaatct cgcctgttgg atgcgacaat gtcgtcatgt
     5041 tgaaggagtt cgaacatcgc tatcgcgccg tacgcggccg cgacgtccgc ttcgacggat
     5101 ggttctacgt cgctgtcacc tccacgggga tctactgccg gcccagttgt cccgccgcga
     5161 ctcccaagcg gtccaacgtc cgcttttatc cgaccgcggc cgccgctcag tacgccggtt
     5221 ttcgcgcctg caagcggtgc aggccggacg ccgtgcctgg ctcaccggag tgggacaaca
     5281 gggccgacat cgtcgcacgc gcgatgcggc tcatcgctga cggcgtcgtc gacagggacg
     5341 gtgtcgcagg gctcgcgcgg cggctcggct acacggagcg tcacctgcac cgtctgctct
     5401 gtgccgacgt tggcgccggt ccgctcgcgc tcgcccgcgc gcgacgcgcc cacacagcca
     5461 ggcttctctt ggagaccaca gacctaccca tcaccgaggt cgcgttcgcc gccggattca
     5521 cgagcgtacg ccagttcaac gacacgatcc gcgaagtctt cgccgtaacc ccgagagagc
     5581 tgcgacgcgt tcgcggacag cacgacgaga cggtgcgcgg ggagatccca ctccgactgc
     5641 cgttcaggaa accgttcgac gcgggcggta tgctgcagtt cctcggtacg cgcgcggtag
     5701 ccggcgtaga gacgttcgac ggggagacgt accgccggac gctgcgtctg ccgcacggcg
     5761 ccggagtcgt ggccctctcc gacggcggcg accacgtcag gtgcgtgctc acgctcgaga
     5821 acgtgaggga ccttggtagc gcggtgcaac gatgtcgttc gctcctcgac ctcgacgccg
     5881 accccgtcgc cgtcgccgac gtgctcgggg cggacccttt tatcggcgca ctcgttcgtc
     5941 gtacaccggg acggcgcgta ccagggagcg tggacggagc cgaactcgcc ttccgggcgg
     6001 ttcttggcca gcaggtctcc gtggccggcg cccggaccct cgccgggcgg ctcgtcgccc
     6061 gctgcggaga gccgctcccc gagacgctcg cagtcgggga cggcgaacct acccatctct
     6121 tcccagggcc gcacgccgtt gccgcggcga cactcgacgg actcggcatc cccggtgcgc
     6181 gtcgcgaaac gttgcgcact ctcgcgcgcg ccattgcagg aggtgggatc ctgctcgatg
     6241 cgggctccga acgcgaggat gtcgaacgac ggttgctgga ggtgcgagga ataggtccgt
     6301 ggaccgcttc atacgttgcg atgcgcgcgc tccgcgaccc cgacgcgttt cttccctcgg
     6361 acctcggtgt cagaaaggca atctccaggc tcggccacgc gggcgaccgg cagagtgtcg
     6421 tcgccgaacg gtggagaccc tggcgcgcct acgccacgca gcacctgtgg gcgagccttg
     6481 gtgacgagaa ggatcggaca gcgaaaggag aggtggtagc atgaaagttt acatcgatga
     6541 ggtagagtcc cctgcaggcc cgctcgcgtt cgcggtaaac gaggatggaa ctctcgtgcg
     6601 gctgcacttc gtagagagcg actacgggcc tgtaatggag gaggatctgc aggacgaagg
     6661 ttacgtcgtg gaagtggacg agaccagaac cgatggcgtg cgaaaagagt tggctgagta
     6721 tgtaagcggg cgtcgcaaga ccttcgacgt gccgctcgcg ctcgccggct cggagtggca
     6781 gaaggccgtc tggacggagt tgacgcgcat cccgtttggg gagaccaggt cgtatgggga
     6841 gatcgcagac tccctcggac ggcctggcgc ggccagggcc gtcggcaggg cgaacgcctc
     6901 caacgtcttg cccgtggtcg taccctgcca ccgtgttatc gccgctgacg gcacgctcgg
     6961 cgggttcaac ggtggcctac acctcaagga gcgcttactt gagcacgagc gtagcgtact
     7021 cgccgcggcg ggcagctagt ttgtggagcg gtcgagaggg caacacaacg tacttctcgt
     7081 ctagaaggag cgtactgtga gaccgagaga tttcgctggt ctcatgctgc taggcgcgct
     7141 atggggcggc tcgttcctgt ttatccgcgt cgccgtgccc gcgttgggtc ccttcgtgct
     7201 cgtagagttg cgcgccggtc tcgctgtcgt ggccctgttt ctctacgccc tcgccgtccg
     7261 cagcatgccg aagatccggg gccggtggag gtcgttcctc gtcctcgggt tcttcaacgc
     7321 ggccttgcct ttctccctta tctccgccgc cgagatacac cttacggcgt ctctcgccgc
     7381 tatcctgaac gcgaccaccg taatgtttag cgcgatcgtt gccgcggtgt ggatgggcga
     7441 cgtcctgacg accagaaaag ccgtcgggat agcccttgga atcgtcgggg tcagtgtgct
     7501 tgttggctgg gacccgctcc ctttgaacgg ggccgtcctg ttggccgtgg cggcgatgtt
     7561 gcttgcttcg ctctcttacg cgctgggggc cacctacgcc aagcgatcgt ttgcggctat
     7621 cccaccgcta gggatggcca taggacagct tggcggggcc acggcgctct tattgcccct
     7681 ggccgtcgtt agtctgcccg aaagggcgcc ttcgaccggc gtaactctct ccatgctagg
     7741 tctggcgttc ctttcgaccg cggtggctta cctgatctac ttccgactca tcgagaacgt
     7801 cgggcccacg agcacactca ccgtcacact actcgtgccc gtcttcgggc tgttgttcgg
     7861 cgtcctgtta ctcgaggagc cgttcgggct tggcacgctg gccgggctcg ggatcatcct
     7921 cacgagcgtc gtccttatca cgggcatcgc tccccggaaa gtgggggccg ttcagcgcgg
     7981 atagaggatc atctgcgtgc atcggaagag cgcaatcggc ttgcctccct cctcgtcgaa
     8041 aacggtcgcg tcccacacct gcgtcgtgcg cccaccgtgg acgagcttcg cttcgcaccc
     8101 gatcgcaccc ccacgtttgg tcccgacgaa gttgctcttc agctcgatcg tcgtgaagct
     8161 ctgtgcgcca tctggcaggt tcacgaatgt tccgtaaccg cacgaggtat ccgcaagggc
     8221 gaccaccgtc gccgcgtgca ggtagccgtt cggggccatg agctcgttac ggagatcgag
     8281 gcggctcgag atcttacctt cctccgcctc cagtatcgtc aacccgatga gaccgggcag
     8341 ggttcccata ccgcgttcgt tcagctcgtc gagagtaggc atctacgggc ctccagggta
     8401 actgtcacgg ctcgagggat tatcccagag gtgaatctcg ttgcttgaga tccacactca
     8461 ggcggtagaa tctactggtg agcgggggca cgaaaacgag atccctcagg gccccacgca
     8521 gggtccgcgc atacctggcc gcgaacgccg ggcgccccgc gctcctgctc gcggcgagcg
     8581 aggaggcggc cgagcggtac gcccgagagg ccgaggtctt caccgacggc cccgtcgtcc
     8641 atctgccctc ccgcggcgcc ccttacgggg acgtcttcga ccccgccgtg agcagggtcg
     8701 gacggcgcca gagggctttg cgttcactgc gttcggcccg cgtcgtggtg gcaggaccgc
     8761 tggcctttat ggaacggacg ccgctctacg agccgatgga actggacgcg ggggcggaga
     8821 tcgagctcga tgcgacgctg gaacgcctcg tcgtcctcgg ctacgagcgg gtcgacagga
     8881 tctcccgccc tggggagttc gccgttcgcg gcggcatcgt agacgtgttc ccgagcacca
     8941 gacgttcgcc cgtgcgtgtg gagtggtggg gcgatgagat agagagcgtc cgcgccgtct
     9001 cgctcgccac ccagcgtgtc gtacgcgagc tggaagccgt aacggtctac gccgcccgcg
     9061 aaggcgacct ggccggcctc gcggccacgg acgggaactt gcccgaggag gcccgccggg
     9121 gcgtgcgggt acccggactc gaccggctcc tgctcgggct gaaccccgtc tcgccgcgta
     9181 cgatgcttcc cgagggcgtc gagatctggc gtgaagagcc acaggaagat gtctccgaag
     9241 tagtagaggg gctcgtccac gagctctacg acagggggat gcccgatccc gacgtcgagt
     9301 tctccctcgg tgaagaagga gagacgattt cggctccacc agtcgcggcg ttcgccgaca
     9361 cgttgaggga ggcggcccgc aggctcgacg cgctcgtcga ggacgggctc gctgtcttcg
     9421 tcgcttgctc ctcacgcggt gaggcgaggc ggacggtgta cgctttcagg gagatccacc
     9481 gcaccatgcg cgaaaccgcg cgggtcgacg cacagttgcc gcccggtctc tacgctcttc
     9541 ccggagaggt cgaggagggg ttcacctacc ccgaaggcgg ggtcgcggtc ttcaggcggg
     9601 actccctgct cggccgcagg cgcgaggcga ggcgccgtac gggcggcagg tcgctcgcgt
     9661 ccttcgcgga tctgaaggtc ggggacctgg tcgtgcacgc catgcagggc atcgggcgtt
     9721 tcgagggcct cgtggccaag gaggtcctcg gatccacccg cgactacatg caggtgagct
     9781 accgcggggg cgacacgctc ttcgtgccat acgagcagat ggagctgttg cacaagtacg
     9841 tgggcggcga cggtgcgcgc ctcgacaagc tcggtggcgc gacgtgggcg caggttacgg
     9901 acagggtccg taagcgggtc aaggccctcg ctggcgagct cctgcggctg cacgcggcga
     9961 gggcctcggc ccccggcttc gctttccccg aggacgggga gtgggagagg gagctcgagg
    10021 agagcttccc ttaccaggag accccggatc aggaatccgc catcaccgcc gtcaagtcgg
    10081 acatgcagag gcctcacccc atggaccgtc tggtctgtgg agacgtcggc ttcggcaaga
    10141 cggaggtctc cgttcgggcc gccttcaagg cggcg
//
LOCUS       DATMVF010000152         8202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_162745, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000152 DATMVF010000000
VERSION     DATMVF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162745"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VN178_07630"
     CDS             <1..1194
                     /locus_tag="VN178_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS80881.1"
                     /translation="TGHGVASPCDGGVLINTSRMKGVRVDPEAQTARVEPGALWADVI
                     SEAQLFGLAGLVGSSSGVGVVGYTMGGGFGWLGRKFGFNADSVREADVVAADGKLLRV
                     SAYEHPDLFWGLKGGGGNFGIVTSMEFALYPLRAVFGGNLFYALERTPEVLDLYSRWV
                     ETLPDEMTTAIAFINFPPLPELPEPLRGGSFVSVRGCYCGEPLEAGEELLRPWREFGE
                     PQVDTFGVMPYQQMDMISMDPPDPLAFYTHVELLGELSPEAIDKLVALAGVESESPLV
                     MLELRQLGGALARPPADLSPIGRRDSQFIMFGLGMSPTPEATPELAQRLQGHLARVAE
                     EMRPYETGATYVNFLDLGDWTPERTRASYSPEDWERLVELKDRYDPTNLFCFNRNIPP
                     SSARQ"
     gene            1406..2290
                     /locus_tag="VN178_07635"
     CDS             1406..2290
                     /locus_tag="VN178_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS80882.1"
                     /translation="MVSPEALKLNEILRNAPKAVDMDLPHQREAGEHAEDMTSEPEGV
                     VFEEAHAVHGLWATPRSWDGESAIMYLYGGGYVISSPHSRRKLAGHLANASGARSLVP
                     RYHLAPERPFPSAVEDATADYRWLLGEGGRPERTVVAGDSSAGGLTVAAMLKLREDRT
                     PLPSGGVPISPWVDLACTGETLETNAAVDLTATKASLQRMAGQYLHGADPRTPLASPL
                     YADLSGLPPLLIVVGGDEALLDDSVRLARAAGMAGVDVTLYIGAGMQHIFPIYCGMIP
                     EADAAVAMIGAWIRSRAS"
     gene            2472..2900
                     /locus_tag="VN178_07640"
     CDS             2472..2900
                     /locus_tag="VN178_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS80883.1"
                     /translation="MKIALMLPVVAGAAIAVQTVLNSLGMRSLGLGGLIGFSGLVTSL
                     VGFVSAAAFLSRPEPSGRALLCALGSGLIGAFVLGAIVLSARAAGLGQTLSVLIASQL
                     LVGLLVDRAGLFGTSVQDLSAAKVLGAALIVVGGVLVVRF"
     gene            complement(3005..3439)
                     /locus_tag="VN178_07645"
     CDS             complement(3005..3439)
                     /locus_tag="VN178_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS80884.1"
                     /translation="MATVDDLDGVIERCQQALREFVKGNPKPMQTMFSHREDVTLANP
                     IAPPARGWDEVAQTMERAASNLREGEIDAFESVARRDTPELAYVVWIERTRGKIGQRD
                     EIVSFPLRVTMIFRPEDDTWKIVHRHADTVTAARPPESMIQE"
     gene            3624..4073
                     /locus_tag="VN178_07650"
     CDS             3624..4073
                     /locus_tag="VN178_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWS80885.1"
                     /translation="MSIEDNTRIVQNVHPLFNERAFDRILSEMIAEDVEWTNVPTAQI
                     FHGLEGMREFMQGWVDAFPDARTEDTTAYAGEEFGVTEFIGRGTHEGPLVSPAGEIPP
                     TGRSVEFRLCEVYQFRDGKVVSGHTHFDSLGLMTQLGVVPPPGQGGS"
     gene            complement(4125..5192)
                     /locus_tag="VN178_07655"
     CDS             complement(4125..5192)
                     /locus_tag="VN178_07655"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWS80886.1"
                     /translation="MPMHRGAVGGIMLPMERDASTAPGRAGRRAMLRTPVCDLLGMEV
                     PIMQAAIWPATDPKLVAAVSEAGGLGSIGAVFESAESVQRQIARVREITPRPFAVNHV
                     VPLLDEAAFQATLEAEPEVVSFALGDPGELVERAHGAGARVIHQIHTVGQARRAAELG
                     VDVIIAQGSEAGGQGMALGVGVMALIPQVADAVDPIPVLAAGGVADGRGLAAALVLGA
                     QGANVGTRFLASDEASAEEVWKRTILQTESEDVVRFDAWEGIFPPAGGGAYATVPRVL
                     RTPFVKEWSGRTREASREAERLRDEVLSAIRGRTTHELLPFSGQTAGLVHDILPAREI
                     VERMVAEAELALTRAGDLKLG"
     gene            5237..6136
                     /locus_tag="VN178_07660"
     CDS             5237..6136
                     /locus_tag="VN178_07660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS80887.1"
                     /translation="MTVGVLRGTRVELPCLFSHLPVTERRQPVELGGENRREPGSYNA
                     PIDYDRVADIYDLYVASDLDIGFYVEEAAKVQGKVLELMCGTGRVSIPLLEAGVDLTC
                     VDLSAGMLARLEERLRARKLEARVVRADVRHLDLGEEEQFDMVIVPFHSFSELVSPRD
                     RELALRAVFGYLREGGRLVCPLHNPPIRARSADGALRFNGSFPTADGGLLLVSGFETL
                     DESSGVVDRLQCYEFYDASNELVAKRVLPMRFALIDRSEFVELADSAGFVPVALYGDY
                     GRKQYSEESSPFMVWIMEKAKNP"
     gene            6355..6708
                     /locus_tag="VN178_07665"
     CDS             6355..6708
                     /locus_tag="VN178_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80888.1"
                     /translation="MHKHGGEQRDRGIPRLGWLIFTALALTISVLVGQGVSAQSTNTC
                     HGSAATIVGTDGPDDLTGTSGQDVVALLDGADSFDGLGGDDVICGVIGKDLLVGAPIT
                     TRSTVAGAMTNCVAT"
     gene            6714..7187
                     /locus_tag="VN178_07670"
     CDS             6714..7187
                     /locus_tag="VN178_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80889.1"
                     /translation="MGTDAIGAGGEDTIIGGPGDDDRTSGDNISLGGEASGDGGDDVI
                     SEVERVTGDNFALQGEASGDGGDDDITGADNAVGDNNGTPGTGTGGNDTIVASAQPNG
                     VLIGDNSDGDSSGGDDHIFGLSGPDFLDGGPGTNECDGGPGKDLAVNCETLVAIP"
     gene            complement(7303..7791)
                     /locus_tag="VN178_07675"
     CDS             complement(7303..7791)
                     /locus_tag="VN178_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80890.1"
                     /translation="MVFLVAAIVNLISPYQRGVFDSFSDYLYQLLVTVAFALIAVAIA
                     GLHALQSRRYGRWGTVGALMAFVGYVIIVVVATAILLAGADVLLSVRLVGAAAILLGS
                     IVLGVMTIRARVLPWWCGVLIIVGFPLGDFSNALIRGARASYSPSCGGWWAWRCCREV
                     AR"
BASE COUNT         1503 a         2576 c         2576 g         1547 t
ORIGIN      
        1 accgggcacg gcgtcgcctc tccctgtgac ggaggcgtcc tgatcaacac ctcgcgcatg
       61 aagggcgtta gggtcgaccc cgaggcgcag accgcccggg tggagccggg tgcgctgtgg
      121 gctgacgtga tctccgaagc acaactcttc gggcttgcgg ggctggtggg ctcttcctcc
      181 ggggtgggcg tcgtcggcta caccatgggc gggggcttcg ggtggctcgg acgcaagttc
      241 ggcttcaacg ccgacagcgt gcgcgaggcc gacgtggtgg cagcagacgg caagctgctc
      301 agggtgagcg cctacgagca ccctgacctc ttctggggcc tcaagggcgg cggaggcaac
      361 ttcgggatcg tcacttcaat ggagttcgcc ctctaccccc ttagggcggt cttcggcggg
      421 aacctcttct acgcgttgga gaggactcca gaggtacttg acctctatag ccggtgggta
      481 gaaacactcc ccgacgagat gaccacggcg atcgcgttca taaacttccc tcccctgccc
      541 gagctccctg agccccttcg cggcggatct ttcgtctcgg tgcggggttg ctactgcgga
      601 gagccgctcg aagcgggcga ggagttgctc aggccctggc gcgagtttgg tgaacctcag
      661 gtagacacat tcggggtgat gccctaccag cagatggaca tgatcagcat ggacccgccc
      721 gatcccctag cgttctacac tcacgtcgag ttgcttggcg agctctcccc ggaggccatc
      781 gacaagcttg tggcgttagc tggcgtagag tcggagtcgc ccctggtcat gctcgaactt
      841 cgccagctcg gcggagcgtt agcacgaccg cccgcagacc tgagccccat cggccggcgc
      901 gactctcagt tcatcatgtt cgggctcggg atgagcccga caccagaggc gacacccgag
      961 ctggcgcagc gtcttcaagg gcaccttgcc cgcgtagccg aagagatgcg cccgtacgaa
     1021 accggcgcca cctacgttaa cttcctcgat ctgggcgact ggactccgga gcgcacacgc
     1081 gcctcctact cgccggagga ctgggagcgc ctggtggaac tcaaggaccg ctacgacccg
     1141 accaacctct tctgcttcaa ccgcaacatc ccgccctctt cggcgcggca gtaaggagct
     1201 tgaaggaaag gaccctactc tcgctacggc gggagcttcc cagatgggcc ttattcaccg
     1261 gagtgcgtag aagatgagtt ctgcgaactt cgcctctacg gagatctgag aagttcgcgc
     1321 taggcaagag tgggtggcat gatgcttgca gcgcgaatgg ttagactgcg cgctctcttg
     1381 gccgatcgga aaggagacac tgacaatggt cagccccgaa gccctcaagc tcaacgagat
     1441 cctgcgcaac gccccgaagg ccgtggacat ggacctaccc caccaacgag aggccggcga
     1501 gcacgccgaa gatatgacct cagagcccga gggtgtggtc tttgaagagg cccacgccgt
     1561 acatggcctg tgggccaccc cgcgatcttg ggacggcgag tcggcgatca tgtacctcta
     1621 cggcggcgga tacgtgatct cctctcccca ctcccgccga aagctcgccg ggcaccttgc
     1681 caacgcctcc ggggcccgct cgctcgtccc ccgctaccac ctagcgcccg agcgcccctt
     1741 cccttcggcg gtcgaggacg ccacggccga ctacaggtgg ctgctcggag agggaggacg
     1801 acccgagcgg acggtagtcg cgggcgactc aagcgccggg ggcttgacgg tcgctgccat
     1861 gctcaagctg cgcgaggatc gcactccgct gccctccggt ggcgttccca tctcgccatg
     1921 ggtggacctc gcctgcaccg gggagacgct cgagacgaac gccgcggtgg acctcacggc
     1981 caccaaggcc agcctgcagc ggatggctgg ccagtacctc cacggggcgg acccgcgcac
     2041 cccgctcgcg tccccgctgt acgccgacct ctcgggcttg ccgcccttgt tgatcgtcgt
     2101 ggggggcgac gaggcgctac tggacgactc ggtacgtctc gcacgcgccg ctggcatggc
     2161 gggtgttgac gtgacgctct acataggggc gggcatgcaa cacatcttcc cgatctactg
     2221 tggcatgatc cccgaagcag acgcggcggt cgctatgatc ggggcgtgga tacgctcccg
     2281 tgcgtcttag agatcgcgaa gcaggttctc tggctctctt cttcgtgtcc ttatccactc
     2341 aagtgcccag aaactcggaa ttctaggaac tgggtacgtg ggggttccaa ggagctcggt
     2401 tcaccccagc ccgttgccgt cgcctggtag gatgctaccg tagtcgtcgt aacgccgagg
     2461 aggaggatct attgaagatc gcgctcatgc tgccggtcgt cgccggggcg gccatcgccg
     2521 tgcagacggt cctgaactcg ctggggatga ggtccctggg cctcgggggg ctcattggct
     2581 tctcggggct cgtcacgagc ctagtgggct tcgtctctgc ggcggcgttc ctctcgaggc
     2641 cggagccgag cggccgggcg ctcctgtgcg ccctgggctc cgggttgatc ggcgccttcg
     2701 tgctgggggc gatcgtgctc tcggcaaggg cggcggggct cggccagacg ctctctgtgc
     2761 tgatcgcctc ccagctgttg gtggggctgt tggtggaccg ggcggggctg ttcggaacct
     2821 ccgtgcagga cctctcggcg gcgaaggtgc tgggcgcggc gctgatcgtg gtgggtggcg
     2881 tgttggtggt gcgcttctaa gcccgtcgtt cggcgactcg atcgttactc aacgaccttc
     2941 gggtaagcct aagaaagcga acttcgcgtg tacgcagttc taagaggttc gctctaggat
     3001 gtatctactc ttggatcatc gattccggcg gacgggccgc ggtcaccgtg tccgcgtgac
     3061 ggtgcacaat cttccaggtg tcgtcctcgg gacggaagat catcgtgacc ctaaggggga
     3121 acgatacgat ctcgtccctc tggccgatct tgcccctggt gcgttctatc cacacgacgt
     3181 aggcgagctc cggggtgtcg cgcctggcta cggactcgaa ggcatcgatt tcgccctccc
     3241 tgaggttcga ggcggcgcgc tccatcgtct gagcgacctc atcccacccg cgggcgggag
     3301 gagcgatggg gttggcgagg gtcacgtcct cccggtggga gaacatggtt tgcatgggtt
     3361 tgggatttcc cttgacgaac tcgcgcagcg cttgctggca tcgttcgatc accccgtcga
     3421 ggtcgtctac ggtagccatc tcgggatcac ctccatcgct ccggcgccct ttccctgagg
     3481 acgctcccag cgatcatcct acgctattca cccgagtgcg tggaagtggg attctccgaa
     3541 gttcacacat agcttgggaa gtggcatcat gatccttggt ggaaagggct gacccgctga
     3601 gcaagcggcc aaaggaggta tcgatgtcta tcgaggacaa cacccgtatt gtccagaatg
     3661 tccatccttt atttaatgag cgtgccttcg atcgcatcct ctcggagatg atcgccgagg
     3721 acgtcgaatg gaccaacgta ccaaccgctc agatcttcca cggccttgag gggatgaggg
     3781 agttcatgca aggttgggtt gatgccttcc cagatgctag aaccgaggac accacagcct
     3841 acgctgggga agagtttggg gtaacggagt ttataggcag gggtactcat gagggtcctc
     3901 tggttagtcc cgctggagag atcccgccga cgggacgttc ggtggagttt aggttgtgcg
     3961 aggtctacca gtttagggat ggcaaggtag tcagcggcca cacccacttt gactcactag
     4021 gactgatgac acagttaggg gtagttcctc caccagggca gggtgggagc tagctgattc
     4081 tcgggccagg gtctaacagc ctcagagctt cttagtgtcc ttaatcaccc gagtttgagg
     4141 tcccctgctc gcgtcaacgc tagttccgct tcggctacca tcctctccac tatctctctg
     4201 gccggcagga tgtcgtgaac caaacctgct gtctgcccgc tgaagggcaa caactcgtgg
     4261 gtggtgcgcc cccggatcgc cgaaagcacc tcatcccgca gccgctccgc ttccctgctc
     4321 gcctctcggg tgcgtcccga ccactccttg acgaaaggtg tacgtagcac gcgcggcacc
     4381 gtcgcgtagg cgccacctcc cgcgggcggg aagatcccct cccaggcatc gaatctcact
     4441 acgtcctcgg actcggtctg gagtatcgtc cgcttccaca cctcctcggc tgaggcttca
     4501 tctgaggcaa ggaagcgcgt gcccacattg gctccttggg cgcctagcac caacgccgcg
     4561 gcgagcccac gtccatcggc caccccacct gctgccaaca ccgggatggg atccaccgcg
     4621 tcggcaacct ggggtatgag cgccataact cccacgccga gcgccattcc ttgcccgccg
     4681 gcttctgagc cctgggcgat gatcacgtcc acgcctagct cggctgcccg gcgggcctgg
     4741 cctacggtgt ggatctggtg gatcaccctc gctcccgcac cgtgtgctcg ctcgacgagt
     4801 tctcctgggt caccgagggc gaaagagacc acctcgggtt ccgcctcgag ggtggcttgg
     4861 aaagctgctt cgtcgagcag cggtaccaca tggttgaccg cgaagggccg aggggtgatc
     4921 tctcgcacgc gggcaatctg tctctggacg ctctcggccg attcgaagac cgcgccgatg
     4981 cttcctaggc cgccagcctc actgaccgcc gcgaccagct ttggatctgt cgcgggccag
     5041 atcgccgcct gcatgatggg aacctccata cccaacaaat cgcatactgg cgttcgcaac
     5101 atggctctcc ttcccgcacg tccgggcgcc gtcgaggcgt ccctttccat cggtagcata
     5161 atgccaccga ctgctccccg gtgcatcggc atacggccaa cgcatccgag tatatagaag
     5221 gggagttccg cgaatattga cggtaggggt cctccgcggt acgcgcgttg agctaccctg
     5281 tctctttagc cacttgccag tgaccgagag gagacaaccc gtggagctag gaggcgagaa
     5341 ccggcgagaa cccggatcgt acaatgcccc catcgactac gatcgcgtgg ccgatatcta
     5401 cgacctctat gtcgcctccg atctcgacat cggcttctat gtagaggaag cagccaaggt
     5461 gcaaggcaag gtcctggagc tgatgtgcgg gaccggacgg gtctccatcc cgttgcttga
     5521 ggccggggtg gacctcacct gcgtggacct ctccgcgggg atgcttgctc gcttggagga
     5581 gaggctgcgc gcgcggaagc tcgaggccag ggtggtgcgg gcggacgtgc gccacctcga
     5641 ccttggcgaa gaggagcagt tcgatatggt gattgtccca ttccattcgt tctccgaact
     5701 tgtctcccca cgcgaccggg agcttgcgtt gagggctgtg ttcggttacc tgagagaagg
     5761 cggccggctc gtctgcccgc ttcacaaccc gcctatccga gccagatctg cagacggcgc
     5821 cctcaggttc aacggtagct tccctaccgc cgacggcgga ctcctcctcg tctccggctt
     5881 cgaaactctg gacgaaagct cgggcgtggt cgacaggcta cagtgctacg agttctatga
     5941 cgcttcgaac gaactcgttg ccaagcgtgt actgcccatg cgcttcgccc tgatcgacag
     6001 aagtgagttc gtcgaattag ccgattccgc gggcttcgtt ccagtggcgc tgtacggaga
     6061 ctacgggcgg aagcaatact cagaggagag tagccccttc atggtatgga ttatggaaaa
     6121 ggcgaagaat ccttaactgg gcactggccc tttatgcacc cgagtgcgta gcaggacaat
     6181 tctgcgaagt tcggaacggc tagcaacact ctagcaacag caaagatgcg ccatttgggg
     6241 gatgcacggc tctctgggtt tgtgtgatga tggtcactag tgacgagtca gcggagcgac
     6301 tttttaccca gtgaggaacg gtactcgtca gatcaactta ccaaaggaag ggatatgcac
     6361 aaacacggcg gagagcagcg cgaccggggt attccacgtc tcgggtggct catcttcacg
     6421 gccctcgcac tcacgataag tgtcctcgtg gggcaggggg tctctgccca atctacgaat
     6481 acctgccacg gcagtgcggc gacgatcgtc ggcactgatg gtccggacga cctaaccggc
     6541 acgtccgggc aagacgtcgt ggcgcttctc gacggcgccg attcattcga cggcctcggc
     6601 ggcgacgatg tgatctgcgg cgtaattggg aaggatctgc tcgtcggcgc cccgataacg
     6661 acacgctcga cggtggccgg ggcaatgacg aactgcgtgg cgacctgagg accgtgggca
     6721 cagacgcgat cggcgcagga ggtgaggaca cgattatcgg cggtcccggt gacgatgacc
     6781 ggactagcgg cgacaacatc tccctcggcg gcgaggcctc gggcgatggc ggcgatgacg
     6841 tgatcagcga ggtggagcgc gtcacaggcg acaactttgc tctccaaggc gaggcctctg
     6901 gcgacggtgg ggacgacgat atcacgggcg ccgataacgc agttggggat aacaacggca
     6961 cgccaggaac cggcaccgga ggaaacgata cgatcgtggc ctctgcccag ccgaatggag
     7021 tactgatagg cgacaactct gacggggaca gcagcggtgg agacgaccac atcttcggcc
     7081 ttagtggacc cgacttcctg gacggcggtc ccggaacgaa cgagtgcgac ggcggcccag
     7141 gaaaggacct ggcggtcaac tgcgagacgc tcgtggcgat cccctgagct caagctgccc
     7201 ctagcttgag gttccgggac gttgccgtag cagcagacta gtcacccaac ctcgtagaaa
     7261 atggattcta cgaggttcag ctcactcgtg aggcttgctc ctctagcgtg ccacttcgcg
     7321 acaacagcgc caagcccacc agcccccaca cgatggcgaa tacgatgccc tcgcccccct
     7381 tatgagggcg ttcgagaaat cgcctagggg gaagccaacg attatcagca cgccgcacca
     7441 ccacggcaat acccgagcgc gtatggtcat gactccaagt actatcgagc cgaggagtat
     7501 cgccgcagcc cctacgagac ggacactcag caggacgtcg gcgcccgcca gaaggatcgc
     7561 tgtcgcgacc acgactatga tcacgtagcc gacgaaggcc atcaaggcgc ccacggtccc
     7621 ccaccgcccg tagcgcctgc tctggagagc gtgcaggccg gctatggcga ccgctatcaa
     7681 cgcgaatgcc acggtgacca acagttggta gaggtagtcg ctaaaggagt cgaatactcc
     7741 tctctggtag ggcgagatca ggttgacgat ggccgctacc aagaacacca ccgcggctgc
     7801 tattccggct aagccgcccc acctaaccaa ctgcgacgac atacccgtgc acttccctgt
     7861 ccgacgccct tgagtggcag ggtcatgatg ccagccgacc aagcccggcg caccaaaccc
     7921 ttatgcacac gagtgcgtgg aaggggttct gcgaacttcg gctttacgca gttctaagaa
     7981 gtttacgccg ctttagcggc gctagcgccg gcttgcgcct ggagttacgg tttcctcgtg
     8041 ggcgaccaga gccatgggtg tatcaccctc gccaaagcgc ctcactctcc ccacgaacca
     8101 gcgcctctgg gtaggcgagt ggcaaggata ttccatggcg aagcactctt ctcggctaga
     8161 gagtaccgaa cgaagcccct caccaaagga tcgggcatac tg
//
LOCUS       DATMVF010000153         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_612987, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000153 DATMVF010000000
VERSION     DATMVF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612987"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1175)
                     /gene="corA"
                     /locus_tag="VN178_07680"
     CDS             complement(207..1175)
                     /gene="corA"
                     /locus_tag="VN178_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWS80891.1"
                     /translation="MIVDNAIYVDGHRTAELITLAETYEAVRNRRGVAWIGLYKPTEE
                     EFSSVAGEFGLHGLAVEDAMDAHQRPKLERYGETLFLVLKPARYLDEEERVEFGEVHL
                     FVGEDFVVTVRHGEAPDLGEVRERLEAAPELLRRGPEAILHAIMDRVVDDYGPVVDGL
                     ENDIEEIEAEVFGGNPSVSRRIYELSREVIEFQRATEPLGGVLGDMMRDEAINVDPEV
                     RRHLRDVRDHVLQVTERLAGFRELLQSILSVNLTLASLAQNEQVKKISAWAAILFAPT
                     LIGTIYGMNFDYMPELGWPLGYPFALLLMAFISLMLYLIFKRRGWM"
     gene            complement(1253..2011)
                     /locus_tag="VN178_07685"
     CDS             complement(1253..2011)
                     /locus_tag="VN178_07685"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS80892.1"
                     /translation="MGKREAYEPGTFCWVDLATSDADGAKTFYGDLFGWEFRDDEIPG
                     GIYSAHGDAIGAIGQQDEHPPHWNNYVSVTSAEETAARAERLGARILEEPFDVGGFGR
                     LAVLVDPAGAMLCVWEPRSHIGAGRVNDVGCMGWNELQTRDSEAAGDFYGELFGWEAE
                     PIEDDGRVVYTTIKNAGNQNGGFMPMTEQHGDAPSFWLPYFTVSSRDATMEKARELGG
                     TVFAGPLDLPSGKIAVLGDPQGAAFAVFEGETDE"
     gene            complement(2740..3498)
                     /locus_tag="VN178_07690"
     CDS             complement(2740..3498)
                     /locus_tag="VN178_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWS80893.1"
                     /translation="MLGATTLWAMVAAFSAGILSGFTGFGLAAVVVPLLLVVYEPKAV
                     VALVAVLSLSVAAAVVRDSWQEADRRVALVLLVPALPGLAVGAEVLRVVDPAYVELAM
                     GTLVVVAAVFLLREDVRLPGAGTRLGAALTGLVSGALASSTGLSGPPAAVLLASRGLP
                     KAAFRASISLYFFGLDLALLVVLGLWGFLDPSLAPLALLLVGVVLVGVALGGKAMGTA
                     LFRRAPRGVFRAVVFGTVVLTGALGVVTAFGPLL"
     gene            complement(3503..>3701)
                     /locus_tag="VN178_07695"
     CDS             complement(3503..>3701)
                     /locus_tag="VN178_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS80894.1"
                     /translation="EIPVLVVWGEKDGWLDPSQAPRLQEQIPGSELELIVGAGHFVME
                     DAPVEVSRILCAFFAEDAERR"
BASE COUNT          682 a         1267 c         1117 g          635 t
ORIGIN      
        1 agccttcctc tccaggcgcg tgggccgtgc ggcgcaggcc ggcccgcaaa gtctgtagcg
       61 tcgaaccctg gatcatcgtc tccatagtcc tctcccttcc caaggttgcg ggccggtagt
      121 ttctcccggc cgggccacac agcggaagca gcctaacctg caatagtaga tgatgcatcc
      181 ccagataagc tattctggaa ggtggcctac atccagccgc ggcgtttgaa gatgaggtag
      241 agcatgaggg agatgaaggc catgagcaat agggcgaagg ggtagccgag aggccagccc
      301 aactcgggca tgtagtcgaa gttcataccg tagatcgtcc ctattagcgt gggcgcgaac
      361 agtatggcgg cccaggcgga gatcttcttt acctgttcgt tctgggccag gctcgccagg
      421 gtcaggttca cgctgaggat gctctgcagc agctcccgga agccggcgag ccgttcggta
      481 acctgcagga catggtcccg gacgtcgcgt agatgcctcc gtacctccgg gtcgacgttt
      541 atggcctcgt cccgcatcat atcccccagc acaccaccga gcggttctgt ggccctctga
      601 aactcgatca cctcgcgcga gagctcgtag atcctgcgcg acacgctggg attaccgccg
      661 aagacctcgg cctctatctc ctcgatgtcg ttctcgaggc cgtcgactac gggtccgtag
      721 tcgtctacga cacggtccat gatggcgtgc aggatcgcct ctggtccacg gcgcagtagc
      781 tcgggggcgg cctccagccg ctcgcgaacc tcgcccagat ccggcgcctc gccgtggcgc
      841 acggtcacca caaagtcttc tccgacaaag aggtggactt ctccgaactc gaccctctcc
      901 tcctcatcca gataacgggc cggcttgagt actaagaaga gcgtctcccc gtagcgctcc
      961 agcttgggcc gctggtgggc gtccatcgcg tcctcgaccg ccagcccgtg caggccgaac
     1021 tccccggcga cggacgagaa ctcctcttcc gtcggcttgt agagtccgat ccatgccacc
     1081 ccgcgccgat ttcggacggc ctcataggtc tccgcgaggg tgatcagctc ggcggtacgg
     1141 tggccgtcca cgtagatggc gttgtccacg atcacggccg cattatacgc ctgcgcttgt
     1201 tgctgctggt acgcttcagc gcgcggcgac gcacccgtac aactgcgcgc ttctactcgt
     1261 cggtctcgcc ctcgaagacg gcgaacgcgg cgccctgggg atcgccgagg acggcgatct
     1321 tgccggaggg gagatccaac ggccccgcaa atacggtacc gccaagctcg cgcgccttct
     1381 ccattgtggc gtcgcgcgag gagaccgtaa agtaagggag ccagaaggag ggcgcatcgc
     1441 cgtgctgctc cgtcatcggc atgaaaccgc cgttctggtt gccagcgttc ttgatggtcg
     1501 tgtagacgac ccttccatcg tcctcgatcg gttcggcctc ccaaccgaag agctcgccgt
     1561 agaaatcgcc tgccgcctcg gaatcacggg tctggagctc gttccagccc atacagccaa
     1621 cgtcgttgac ccttcccgct ccgatgtgcg accgcggctc ccagacgcag agcatcgcgc
     1681 cggcggggtc gactagcacg gccaggcggc cgaacccacc cacgtcgaaa ggctcctcga
     1741 ggatcctggc gcccagccgc tccgcccttg cggcggtctc ttcggcgctc gtgacggaga
     1801 cgtagttgtt ccagtgcggt gggtgctcgt cctgctggcc gatcgcacca atcgcgtcgc
     1861 cgtgggccga gtagatgccg ccggggatct cgtcgtccct gaactcccag ccaaacagat
     1921 caccgtagaa ggtcttcgcg ccatcggcgt cagaggtcgc aagatccacc cagcagaacg
     1981 tccccggctc gtaagcctct ctcttgccca tcggtctatc tccctttttc gccacttcgg
     2041 accggccgaa aggccgtatc gggagtctac gagaacaccc cccattccca accggcaaac
     2101 gtctgagatg gagcctggct ctccgagatt cgatacgacg ccaggggtct tgatgggatg
     2161 tgccttctag tgaagcgtgt tcctgggcta tctttcggtc gacgccaccg tgcgatcttc
     2221 tcgaaggtca tcggggtatg ctgctctgtt cgtgacgggg aaggtcgtcg cgaacgtgaa
     2281 accagggaat ccgctgggaa acccaatcgt gtgcctcggg ttcgaacgct gccggatctt
     2341 cgaagattcc aacgggaacg tacaccttcc ccggtagcct ctcgtcttcg taggagaggg
     2401 gcgtcccgca agcgggacag aatgacctta ccaccgtcgc ggaagaccgg tagcctttag
     2461 gctcgccccg tgaccactcc acttgctcgg cgcggtagcc ggcaaacaag gatatgggtg
     2521 cgccattaga ggctcggcag tcgcggcagt gacagtacac taccacctgg ggtacgccgg
     2581 ccgttcgcag ccctactgcg ccacacatgc accctcccgt acgaccaggc gccatgctcc
     2641 tctcgttcat gcggttgtac ctcctcatca atccgtaggt gcttctgctc gaccttctcg
     2701 cttgtctccg ctttcgttcg gattccacgg gagcgctcgt tatagtagcg gcccgaaggc
     2761 ggtgacgacg cccaaggctc ccgtaaggac gacggtaccg aagacgacgg cgcggaacac
     2821 cccgcgggga gcccgcctga acaacgcggt ccccatcgcc ttgcctccga gggcgacgcc
     2881 cacgagcacg acgcccacga gcaataacgc caggggcgcg agagacggat ccaagaatcc
     2941 ccagagcccc agtacgacca atagcgcgag gtccaacccg aagaagtaca gagagatcga
     3001 ggcgcggaac gccgccttgg ggaggccgcg cgaggcgagt agcaccgccg ccggggggcc
     3061 ggagaggccc gtggagctgg cgagggcgcc gctcactagc cctgtgagcg cggcacccag
     3121 ccgcgtcccg gcgcccggca gacgtacgtc ttcgcgcagc agaaagaccg ccgcgaccac
     3181 cacgagcgta cccatcgcca gctctacgta ggcggggtcc acgacccgca agacctccgc
     3241 gcccaccgcg agtccaggca aggccggaac gagaaggact agtgccacac gtcggtcggc
     3301 ctcctgccag gagtcgcgca ccaccgccgc ggccacggac agggagagca ccgccaccag
     3361 cgcaacgacc gccttgggtt cgtagaccac caggagcaac ggcacgacga ccgccgccag
     3421 accgaagccg gtgaacccgc tcaagatccc ggccgaaaac gccgctacca tcgcccagag
     3481 ggtggtggcg cctagcatgg tctcaccttc gctctgcgtc ctcggcgaag aaggcgcaga
     3541 gaatcctcga gacctccaca ggggcgtcct ccatcacgaa gtggccggcc cccactatca
     3601 gctcgagctc tgaacccggg atctgctctt gcagcctcgg agcttgcgag ggatctagcc
     3661 acccgtcctt ctcgccccac accacgagaa cggggatctc g
//
LOCUS       DATMVF010000154         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_485466, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000154 DATMVF010000000
VERSION     DATMVF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485466"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VN178_07700"
     CDS             <1..1159
                     /locus_tag="VN178_07700"
                     /inference="COORDINATES: protein motif:HMM:NF036810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80895.1"
                     /translation="APLAVAEGFVRHVVACGGELKNTFCVAKECHVFLSHHIGDLENY
                     ETLTSFREGVEHFCRLFDVQPELVVYDLHPEYLSTKYARELEEAGLPAVGVQHHHAHV
                     ASCLADNERPCEERVIGVALDGTGYGTDGAVWGGEFFEGSIEEGYVRQGHLEYAPMPG
                     GSAAIRQPWRMALAQLIALYGEDETLKLPLATVHRAGERNARLVARLVEHSLNTPPTS
                     SAGRLFDAVAAMIGVPGTERTTYEGQAAVELELAAHGTASRGYPFRLRPEGQGWIVET
                     QRILCGVVEDLLAGRATGEISSRFHRTMAEVVVAGCERIREAGGTTAVALSGGTFQNM
                     LLLNQVVRFLEEKGFEIYRHRRVPPNDGGLALGQAILANKASGHRHQEEKT"
     gene            1188..1466
                     /locus_tag="VN178_07705"
     CDS             1188..1466
                     /locus_tag="VN178_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HWS80896.1"
                     /translation="MCLGIPGRIVEIIDEDLTLAKAEVSGVRRNINIGLVHYDEERVE
                     IGDWVLIHVGFAMSKIDEKEAYATLRALEDIGEDYEQELEELKTSKIE"
     gene            1468..1722
                     /locus_tag="VN178_07710"
     CDS             1468..1722
                     /locus_tag="VN178_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HWS80897.1"
                     /translation="MKDDLTLLGNCTLDHDGCVVCSDAGIPVRVISIEGDDALCEDSA
                     GNQTEIAIELVAPVQIGEVLLTHGGVAIGKVETKELTAES"
     gene            1758..2882
                     /gene="hypD"
                     /locus_tag="VN178_07715"
     CDS             1758..2882
                     /gene="hypD"
                     /locus_tag="VN178_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HWS80898.1"
                     /translation="MKYVDEFRNADLARAVSAEIASLVDPKRHYKIMEVCGGHTHTIY
                     RHGIKDLLPDQVELVHGPGCPVCVLPMGRVDEGIAIAERPEVIFTCFGDMMRVPGSKG
                     NLLEVKAEGADIRMVYSPLDALRIARENPDREVVFYAIGFETTAPSTALTLQRAKKEG
                     IENFSVFCNHVTIVPPVRALLDSPDLRLDAFIGPGHVSTVIGCRPYEFMPADYGKPCV
                     VAGFEPLDLMQSVLMLIRQLDEGRCEVENQYGRAVLWEGNLKALEAMSEAFELRPYFE
                     WRGLGFIAQSALKLSPAYADLDAELRYSTPGVRVADPKACQCGEVLKGVLKPPECKVF
                     GTACTPERPLGALMVSSEGACAAYYNFGRKSFEKVEIGAR"
     gene            2879..>3800
                     /gene="hypE"
                     /locus_tag="VN178_07720"
     CDS             2879..>3800
                     /gene="hypE"
                     /locus_tag="VN178_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HWS80899.1"
                     /translation="MSEGREKVGAGFGPVPTDALQQNRRRSPRFTAPRIDMSHGAGGK
                     ATHKLIEGLLLPAFDNPALAPLADAAMLPFGDTSLAISTDSYVVRPLVFPGGSIGELA
                     VNGTVNDLAVSGATPLALSAALIVEEGLETDALAHEIEAMAAAARNAGVSIATGDTKV
                     VERGKADGLYITTTGVGMVDEGLDLSASSVRPGDKVLCSGTLGDHGAAILIARGDLEL
                     EAEVLSDTRPLTPLVEALKSVAGQGLRFMRDPTRGGVGTVLNELARDSGYGVALWEER
                     IPVREVVNGACEILGIDPLYVANEGKLLAVM"
BASE COUNT          752 a         1209 c         1237 g          602 t
ORIGIN      
        1 ggcgccgctg gccgtggcgg agggcttcgt gcggcacgtc gtggcttgtg gtggcgagct
       61 caagaacacc ttctgcgtag cgaaggagtg ccacgtcttc ctcagccacc acatcggtga
      121 tttggagaac tacgagaccc tgacatcctt ccgggagggg gtcgagcatt tctgccggct
      181 cttcgatgta cagccggaac tggtggtcta cgatctccac cccgagtatc tctccaccaa
      241 atacgcccgc gagctggagg aagcgggatt accagccgtg ggcgtacagc accaccacgc
      301 gcacgtcgct agttgtctgg ccgacaacga gcggccctgc gaggagcgcg tcatcggggt
      361 agccctggac ggaaccggct acgggacaga cggagctgtc tggggagggg agttcttcga
      421 ggggagcata gaagaggggt acgtacggca agggcacctg gaatacgccc ctatgcccgg
      481 tggatcggcg gccatccggc agccgtggcg gatggcgcta gcgcaactca tcgcgctcta
      541 cggcgaagat gagaccctga agcttccgct cgcaaccgtc caccgggctg gcgagcggaa
      601 cgcgcggctc gtcgcccggc tcgtggagca cagtctcaac accccgccga cctccagcgc
      661 aggacggctc ttcgacgccg tcgcggcgat gatcggagtg cccggaacgg agcggaccac
      721 ctacgaggga caggctgcgg tggagctgga actcgccgcg cacggtacgg cgagtcgggg
      781 ttatcccttc cgactgcggc ccgagggcca aggatggatc gtggagaccc agaggatcct
      841 ctgcggggtc gtggaggatc tcctggcggg tagagcgacg ggagagatat cttccaggtt
      901 ccaccgtacg atggccgaag tcgtcgtcgc cggctgcgag cgcatccggg aagccggagg
      961 aacgaccgcc gtcgcgctct ccggcggtac attccagaat atgctccttc tgaatcaggt
     1021 ggtacgattc ctggaggaga aagggttcga aatctaccgg caccgccgcg tgccgccaaa
     1081 cgacggaggt ctggcactag gtcaggcaat cctggcaaat aaggcttcag gccacaggca
     1141 tcaggaagag aagacctgat acctgagcga ccgaagggag cgaacacatg tgtctgggga
     1201 taccgggacg gatcgtggag atcatagacg aggacctgac gctcgccaaa gcagaagtca
     1261 gcggcgtccg gcgcaacata aacatcggcc tggtccacta cgatgaggag cgagtagaga
     1321 tcggggactg ggtcttgatc cacgtgggct tcgccatgtc caagatcgac gagaaagaag
     1381 cctacgccac cctgcgcgcc ctcgaagaca taggcgaaga ctacgagcaa gagcttgagg
     1441 agctcaagac ctcaaagatc gagtgacatg aaagacgacc tgacgctgct cggaaactgc
     1501 accctggacc acgacggatg cgtcgtctgc tccgacgccg gcatccccgt ccgcgtcatc
     1561 tccatagaag gcgacgacgc cctctgcgaa gactccgcag gcaaccagac cgaaatagcc
     1621 atagaactcg tcgcccccgt gcaaatcgga gaggtcctgc taacgcacgg aggcgtcgcc
     1681 atcggcaaag tagagaccaa agagctgacg gctgaaagct gacggctgat agctgtgacg
     1741 accgtaggga ggaaccaatg aaatacgtcg acgagtttcg gaacgcggat ctcgcgcggg
     1801 cggtgtcggc cgagatcgcc tcgctcgtag acccgaagcg tcactacaag atcatggagg
     1861 tctgcggcgg gcacacccac accatctacc gccacgggat caaagaccta ctgccggatc
     1921 aggtagagct ggtgcacggc cccggctgcc cggtctgcgt gctcccgatg ggccgggtgg
     1981 acgaggggat agccattgcc gagaggccgg aggtcatctt cacctgcttc ggggatatga
     2041 tgcgcgtccc cggctcgaaa ggaaacctgc tcgaagtgaa agccgaaggc gccgacatcc
     2101 gcatggtcta ctcgccgctg gacgccctgc gaatagcccg tgagaaccct gaccgggaag
     2161 tcgtcttcta cgccatcggg ttcgagacca cggccccctc cacggcactc acgttgcagc
     2221 gggcgaagaa ggagggcatc gagaacttct ccgtgttctg caatcacgtc accatcgtgc
     2281 cgccggtgcg cgcgctcctg gactcgccgg acctgcgcct cgacgccttt atcgggcctg
     2341 ggcacgtctc gacggtgatc ggctgcaggc cctacgaatt catgcccgcc gactacggca
     2401 agccgtgcgt cgtagcgggg ttcgagccgc tggatctcat gcagtccgta ctgatgctca
     2461 tacgccagct cgacgaaggt cgctgcgagg tcgagaacca gtacggccgg gccgtcctct
     2521 gggaagggaa cctcaaggcg ctcgaggcca tgtccgaggc cttcgaactg cgcccgtact
     2581 tcgagtggag ggggctcggc ttcatcgccc agtccgcctt gaagctctcc cctgcatacg
     2641 cggacctgga cgcggagttg cgctactcca cgccaggcgt gcgagtcgcg gacccgaaag
     2701 cctgccagtg cggcgaggtc ctcaagggcg tgctcaagcc gcctgagtgc aaagtcttcg
     2761 gcacggcctg tacaccggag cgtccactcg gcgcactaat ggtctcctcc gaaggcgcct
     2821 gcgccgccta ctacaacttc gggcgcaagt cgttcgagaa ggtcgagatc ggcgcccgat
     2881 gagcgagggc agggaaaagg taggggcggg tttcggaccc gtccctacgg acgcgctgca
     2941 gcagaaccgc cgcaggtcgc cgaggttcac agccccacgc atagacatga gccacggcgc
     3001 cgggggtaag gctacccaca agcttatcga gggtctcctg ctcccggcct tcgacaaccc
     3061 ggcgctcgcc ccgctcgccg atgccgccat gctccccttc ggcgacacca gtctcgccat
     3121 ctccaccgac tcctacgtcg tgcgccccct cgtgttcccc ggcgggtcca taggggagct
     3181 cgcggtcaac gggacggtca acgacctggc cgtctccggc gccaccccgc tcgcgctctc
     3241 cgccgccctc atcgtcgagg aggggttgga gaccgatgcg ctggcacatg aaatagaggc
     3301 gatggccgcc gccgccagga acgccggggt ctccatagcc accggggaca ccaaggtggt
     3361 cgagcgcggc aaggcagacg ggctctacat caccaccacc ggcgtcggaa tggtggacga
     3421 agggctcgac ctctccgcct catccgtgcg tccgggagac aaggttctgt gctccggtac
     3481 actgggggat cacggcgcgg ccatactcat cgcgcgcggt gacttggagt tggaagccga
     3541 agtcctctcc gacaccaggc ccctcacccc actggtcgag gccctcaaga gcgttgcagg
     3601 acagggactg cgcttcatgc gcgaccccac acgaggcgga gtcggaaccg tcctcaacga
     3661 gctcgcccgc gattccggct acggggtggc tctgtgggag gagagaatcc cggttcggga
     3721 ggtggtgaac ggagcttgtg agatcctggg catagacccg ctctacgtcg caaacgaggg
     3781 caaactcctc gccgtaatgg
//
LOCUS       DATMVF010000155         7109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_347455, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000155 DATMVF010000000
VERSION     DATMVF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347455"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /gene="dut"
                     /locus_tag="VN178_07725"
     CDS             <1..408
                     /gene="dut"
                     /locus_tag="VN178_07725"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWS80900.1"
                     /translation="EAGMPERAHPGDAGYDLRSVERVDLAPGARALVRTGISVAIPEG
                     YAGLVLPRSGLAIRHGISLVNTPGLIDSGYRGEIQVPLINHDREETFLIEEGTRIAQL
                     VLVRAAAAFFAEVQFLEPATDGRGESGFGSSGS"
     gene            410..874
                     /locus_tag="VN178_07730"
     CDS             410..874
                     /locus_tag="VN178_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HWS80901.1"
                     /translation="MRFLCDAMLGGLAKWLRAAGYDAYYAREGTDVSDGTLTAKALAE
                     RRVLLTSDGGFLERKPVRDGSVGFLRVPHLPLEDQLRLVVEHFGLVRRQSRCMVCSGS
                     LDIVPREVVAGRVPSGVQRDHERFFLCQDCGRIFWHGSHWQRISDRLDRVFA"
     gene            979..1548
                     /locus_tag="VN178_07735"
     CDS             979..1548
                     /locus_tag="VN178_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWS80902.1"
                     /translation="MNATTHAVFGIAALAGAALVTGADPPVYVYPAAVAAAWLPDIDN
                     PRSTLGNGLNRLKNPTLNVLSRPLSWALRTMSFVLVRTVGHRTMTHSVFGVLLFSLLV
                     LLFLGRFPNLALALVAGYASHVFADALNTRGVPLLWPIGKPFRLLPGGVRSGGAVEVA
                     VALVALAFTLYALLLLYPGLRGAIGLQPI"
     gene            complement(1498..2109)
                     /gene="yqeK"
                     /locus_tag="VN178_07740"
     CDS             complement(1498..2109)
                     /gene="yqeK"
                     /locus_tag="VN178_07740"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HWS80903.1"
                     /translation="MGDHAKIHTLLERAEAFARSRLSKERYEHTLRVADTAEDLALAH
                     DLEADRLRLAALLHDAAREIGREEFLELAQEWHLQVGDAERQSPKLLHGPVAAELARR
                     ELGVDDEEVLEAVRAHTTGRPGMEPLALVLYVADKIEPARDYPSVGRLRTLAREDLNR
                     AAEESLRRAIAHNEERGKATHPASLAMLDWLEADRTAQSRIEK"
     gene            2133..3401
                     /locus_tag="VN178_07745"
     CDS             2133..3401
                     /locus_tag="VN178_07745"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HWS80904.1"
                     /translation="MRDGQTIQSAEIVTVGTEMLLGDLVDTNSAWISQRLAGLGVGIY
                     RHTTVGDNPERLVAALREAASRSDLVVTTGGLGPTSDDLTNACISTLTGREMVEYPEA
                     REHVDRMFQKFGRKPTANNYKQALFPQGTELIPNPVGTAMGALVEWEGTLFATLPGVP
                     PEMKSMFEATLEPMIRARSEGSIVSKTLHFAGIGESALAEKVQEFLDATDPTVAPLAG
                     QGRVRLRITTRAATEKEAQEKIAPIEREMIARLSEYYFGEDDETLEGAVGRLLQERGA
                     KLALAESCTGGLLAKRLTDMPGSSAYFQEGLVTYSNESKERLLGVPHDLIVEHGAVSE
                     PVACEMAEGARRVSGADYGLSVTGVAGPDGGTEEKPVGLVFVGISDSNNTFAEKLDLT
                     AWARSRESIRERSANRAFDLLRLYLEDEQS"
     gene            complement(3685..4815)
                     /locus_tag="VN178_07750"
     CDS             complement(3685..4815)
                     /locus_tag="VN178_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HWS80905.1"
                     /translation="MGESSGTGFVAGLEDVVAAETRLSGVDGEAGELIIGGFPVEELA
                     GRASFEETVYLLRHDVLPDSGRLADFREELAGWRELGPATLDLLRTVAVEKIPAMDAL
                     RMAAGTVNPGTDEEEALALVARLPTIVAAYSRMLEGEEPVVPDPKLGHAANYLYMLTG
                     EVPGAEFVRALDTYLTTVSDHGMNASTFAARVIVATRSDFVSAVVGAIGALKGPLHGG
                     APGPALDVVFEIGDASRAEAYLREKLGRGERLMGFGHRIYRVRDPRADVLAAAAERLY
                     TTDGDQELYDLALDVEQTALTLLAEHKPGRNLQTNVEFYTALLLHGLGLPAQLFTPTF
                     AAGRVAGWTAHCFEQRAIDRLIRPQSVYVGGRDGSWVPLRER"
     gene            4969..6201
                     /locus_tag="VN178_07755"
     CDS             4969..6201
                     /locus_tag="VN178_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HWS80906.1"
                     /translation="MREERYLGSGQAAEELGISLATLYAYVSRGMVRSEAVEGKGRAR
                     RYRAEDVRRLKERKERRRDPDGVVEGALHWGTPVLESGITMIDGGVLYYRGRDVLDLA
                     EENTIEEVAALIWTGDEAMAPALFPPEMSGPSQRIQSVVGSVAGLTPGEVFQVLLPVA
                     AAEDPAAYDLRPGAVARTGARILRLMTDVASGESAPGLAGTLQRGWRTDDPGAESLLG
                     SALVFCVDHELPVSTFAARCVASSEATPYAVVLAGLAALGGVKHGGEIELALAFLREV
                     EGAGDARAVISGRLRRGERIPGFGHSLYPEGDPRGAGLLRLTAGAYPESIAVALSESV
                     AGEMLRLMDERPTVDFALATVARTLGLPAGGAVALFALGRTVGWIGHAIEQYRSGSLI
                     RPRARYVGEQPSRQPDKR"
     gene            complement(6237..6959)
                     /locus_tag="VN178_07760"
     CDS             complement(6237..6959)
                     /locus_tag="VN178_07760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWS80907.1"
                     /translation="MVDSQDALLSFVVMGAGVAGAVVGGFWLAVFWMRSLGKKVGYSL
                     APLGFSRPRGGLLAGICAGMAVGVGAVLLGMIVNPISIFVLERLGYSTESTVQQPFMQ
                     GLVGWVQESPGVAIPAIILIVVLFGPAVEELVFRGVVFNGLYRLGALIYARSVGAEHS
                     RRRPGRMIFISSALASSVLFALLHLEPVLLPVLLILAITLCALFQRTGSLLPSFVAHA
                     TFNSVAAVLIILSGLDVLNIQI"
BASE COUNT         1262 a         2319 c         2272 g         1256 t
ORIGIN      
        1 gaggctggga tgcccgagcg cgcccatccg ggggatgcgg gctacgatct gcgttcggtc
       61 gagagggtgg atctcgcgcc gggagcccgg gcgctggtgc ggacggggat ctctgtagct
      121 atacctgagg gttacgctgg actcgtgctc ccgcgcagcg gcctggccat caggcatggg
      181 atctccctcg taaacacgcc tggtctaatc gactctggct accgcggtga gatccaggtc
      241 cccctgataa accacgaccg tgaggaaacg ttcctgatcg aggagggaac caggatcgcg
      301 caactggtcc tcgttcgggc cgccgcagcg ttcttcgccg aggtacagtt cctggagccg
      361 gcaaccgacg ggcgggggga gagcggcttc ggctcctccg gatcctaggg tgaggttcct
      421 ctgcgacgcg atgcttggcg ggctggcgaa gtggctgcgc gccgccgggt acgacgccta
      481 ctacgccagg gaggggacgg acgtctcgga cgggaccctc accgcgaaag ctctcgcgga
      541 gaggcgcgtg ctcctgacca gcgacggggg cttcctggaa cgcaagcccg tacgtgacgg
      601 gagcgtgggc ttcctcaggg tgccgcacct cccgctcgaa gaccagctca ggctcgtggt
      661 cgaacacttc gggctcgtca ggcgccagag ccgctgcatg gtatgtagtg ggagcttgga
      721 catcgtaccc cgcgaagtcg ttgcgggccg ggttccgtcc ggcgtacaga gggatcacga
      781 acgcttcttt ctctgccagg actgcggacg catcttctgg cacggctcgc actggcagcg
      841 tataagcgac cgcctcgaca gagttttcgc ttgagtgaga agcccggcaa gtacgaggag
      901 caccggcatg ttctcgattg acgagccgtc gccgcagcgg atcgcccgtt tccgtcgagt
      961 tgctaaactg cttccggatt gaacgcgacc acccacgccg tcttcgggat tgcggccctg
     1021 gccggcgccg cgctcgtcac gggggccgat ccgcccgtct acgtctaccc tgccgccgtc
     1081 gccgccgcct ggctgcccga catcgacaac ccgcgcagca ccctgggcaa cggcctgaac
     1141 cggctgaaaa atcccacact caacgtactg agccggccct tgagctgggc cctgaggacg
     1201 atgtccttcg tgctggtcag gaccgttggc catcgcacca tgacccattc ggtgttcggt
     1261 gttctactct tttcgctttt ggtcctgctg ttcttgggac gtttcccgaa cctcgccctg
     1321 gccctcgtcg ccggctacgc ctcgcacgtt ttcgccgacg cgctcaatac gcgcggcgtg
     1381 ccgcttctct ggcccatagg taagccgttc aggctcttgc ccgggggtgt gagatccggc
     1441 ggggccgtgg aggttgccgt ggccctcgtg gccctcgcct tcacgctcta cgccctgcta
     1501 cttctctatc ctggattgcg cggtgcgatc ggcctccagc caatctagca tcgccaggct
     1561 ggcaggatgc gtggccttcc cccgctcttc gttgtgggct atggcgcgcc gcaacgactc
     1621 ctcggctgcc cggtttaggt cctccctggc cagagtacgc aggcgtccca cgctcgggta
     1681 gtcgcgggcc ggttcgatct tgtccgcaac gtaaagaacg agcgcgagcg gctccatccc
     1741 cggcctccct gtcgtgtggg ccctgacggc ctccagaacc tcctcgtcat cgacgccaag
     1801 ctcgcgtctc gccagctcgg cggccaccgg cccgtgcagc agcttggggc tctgccgttc
     1861 cgcatctccc acctgaaggt gccattcttg ggccaactcg aggaactcct cgcgccctat
     1921 ctcgcgtgct gcgtcgtgaa gcagggcggc caggcgcaat ctatcggcct cgagatcgtg
     1981 ggccagtgcc agatcctcgg ccgtgtcggc caccctcagg gtgtgttcgt aacgctcctt
     2041 cgagagcctg gaacgggcga aggcttcggc tctttcgagg agagtgtgaa ttttggcgtg
     2101 gtcccccatc cgacggattg tagaatacct gcgtgcgcga tgggcaaact atacagagcg
     2161 ccgagatcgt aaccgtgggt accgagatgc tcctcgggga cctcgtggat acgaactcgg
     2221 cctggatcag ccagcgcctc gccggactcg gggtgggtat ctaccgccac accaccgtcg
     2281 gggacaatcc ggagaggctc gtcgcagccc tgcgcgaggc cgcctccaga tccgacctgg
     2341 tggtcaccac aggcggcctc ggccctacat cggatgacct gaccaacgcc tgcatatcga
     2401 ccctgacagg ccgcgagatg gtcgagtacc ccgaggccag ggagcacgtg gacaggatgt
     2461 tccagaagtt cggccgcaag ccgaccgcca acaactacaa gcaggccctc tttccccagg
     2521 gaaccgagct catccctaac cctgtgggaa cggcgatggg ggctctggtg gagtgggaag
     2581 ggacgctgtt cgccacgcta cctggggtgc ccccagagat gaagagcatg ttcgaagcga
     2641 cgctcgagcc catgatccgg gcccgcagtg aaggctccat cgtctccaaa accctccact
     2701 tcgccggcat cggggagtcc gcgctcgccg agaaggtcca ggagttcctc gacgccaccg
     2761 accccacggt cgccccactg gctggccagg gcagggtgcg tctgcgaatt accaccaggg
     2821 ccgccaccga gaaggaggcg caggagaaga tagcgccgat agaaagagag atgatcgcac
     2881 gcctcagcga atactacttc ggcgaggacg acgagactct cgaaggcgcg gtcggccgcc
     2941 tgctccagga gcggggcgcc aaactggccc tcgcggagag ctgcacaggc ggcctcctcg
     3001 ccaagcgcct gacggacatg ccgggctctt cggcgtattt tcaggaggga ctcgtcacct
     3061 actcaaacga gtccaaagag cgtctgctcg gggtgccgca cgacctgatc gtggaacatg
     3121 gcgccgtctc ggagccagtg gcgtgcgaga tggccgaggg ggcgcgcagg gtctcgggcg
     3181 ccgactacgg gctctcggtc accggagtag ccggccccga cggcggaact gaggagaagc
     3241 ccgtcgggct cgtcttcgtc ggaatctccg actcaaataa cacgtttgcg gaaaagctcg
     3301 acctcaccgc ctgggcccgc tccagggaat cgatccgcga gcggagcgcg aaccgggcct
     3361 tcgacctcct caggctctac ctggaagatg aacaatcttg acgatcaccc ttgctggata
     3421 caatgcgcgg tagtaaactt cgcggagttg aaggcacgcg aaaggaggtg atccagatag
     3481 acgcatcgag tagtagtcgc gggtatcttc tgggagaggt ggctctgacc taggcccacc
     3541 aaaacacccg gcgggatcct gggttacttc atccgcctcc catgtagtgt aggcggacgt
     3601 gctgaaagac gggatacctg cgtaacgctc agaagggtac ggcccccgga cttctccggg
     3661 ggtcgttttt cgtatctccc cacactaccg ctctcggagc ggcacccaac ttccatccct
     3721 gcccccaacg tacacggact gcggcctgat caggcggtct atcgcccgct gctcgaagca
     3781 atgcgccgtc cacccggcca ccctccccgc cgcgaaggtc ggggtgaaga gctgcgcggg
     3841 caacccgagc ccgtgaagaa gtagcgccgt gtagaactcg acgttggtct gcaggttcct
     3901 gccgggcttg tgctcggcga gcagcgtgag cgctgtctgc tccacatcga gcgccaggtc
     3961 gtagagttct tggtctccgt cggtggtgta aaggcgctcg gcggcggcag cgagtacgtc
     4021 tgccctcggg tcgcgtacgc ggtagatcct gtgcccgaaa cccatgagcc gctcgccgcg
     4081 cccgagcttc tcccgcaggt acgcctcggc cctgctcgcg tccccgatct cgaacaccac
     4141 atcgagcgca ggccctggtg cgccaccgtg caaaggcccc tttagcgctc cgatcgcccc
     4201 gaccacagcg gagacgaagt ccgaccgcgt ggcaacgatg accctcgccg cgaaggtgga
     4261 ggcgttcatc ccgtgatccg acacagtagt gaggtacgta tccagggccc gcacaaactc
     4321 tgcgccaggc acttcacccg tgagcatgta cagatagttg gccgcatggc ccagcttggg
     4381 atcaggcacc acaggctcct cgccctcgag catgcgcgag tacgccgcca cgatagtagg
     4441 cagacgcgcc accagcgcca gcgcttcctc ttcgtccgtg ccagggttca cggtccctgc
     4501 cgccatgcgc agcgcatcca tcgccggtat cttctctacg gctacagttc gcagcagatc
     4561 cagcgtagcc ggccccagct cccgccaccc cgcaagctcc tcccgaaaat ccgcgagtcg
     4621 cccggaatca ggtaagacgt cgtgccggag caggtacacg gtctcctcga aggaagcccg
     4681 ccccgccagc tcctcgaccg gaaagccacc aatgatcaac tccccagcct caccgtcgac
     4741 accgctcagg cgggtctcgg ccgccaccac atcctccagc ccagcaacga aacctgtccc
     4801 agaactctcg cccatgccaa tcctttcatg cctgacccgg attcccgagt cgccgtcatg
     4861 gtcatcaatc gtaactctcc atgaaatatg tatcaacgtt gattatcgaa tcaatacgta
     4921 gtagggttgc ggtcgtgtgg cggtacaccc gggtgaggag gctttcggat gagagaggag
     4981 cggtatctcg gctctgggca agcggcggag gagttgggga tcagtcttgc gactttgtat
     5041 gcgtacgtga gcagggggat ggtacgttcc gaggcggtag aagggaaggg gcgcgccagg
     5101 cgttacaggg ctgaggacgt aaggaggctc aaggagcgca aggagcgcag gcgggaccct
     5161 gatggtgtgg tagaaggtgc cctgcactgg ggcacgccag tgctcgagtc ggggatcacc
     5221 atgatcgacg gtggcgtact gtactacagg ggacgcgacg tcctggatct cgccgaggag
     5281 aatacgatcg aggaggttgc ggccttgatc tggacgggcg atgaggcgat ggccccggcg
     5341 ctcttcccac ccgagatgtc cggaccctcc cagcgtatcc agagcgttgt gggcagcgtg
     5401 gccggactaa cgcccggcga ggtcttccag gtcctgctcc ctgtggcggc cgccgaggac
     5461 ccggccgcct acgatttgcg acctggcgcc gtcgcgcgca ccggcgccag gatactccgc
     5521 ctgatgacgg acgtggcctc gggagagtct gctcctggcc tggcggggac gcttcagcgt
     5581 ggctggagaa cggacgatcc aggggccgag tcgcttctcg gctccgcgct cgtgttttgc
     5641 gtggaccatg agttacccgt ctcgaccttc gctgccaggt gcgtcgcctc gtccgaggcc
     5701 acgccgtatg cggtcgttct ggccgggctt gcggcgctcg ggggcgtcaa gcacgggggg
     5761 gagatcgaac tcgccctggc ctttttgcgg gaggtggaag gtgctgggga tgcacgggcg
     5821 gttatctccg gccgtctcag gcggggggag agaatccctg ggttcgggca ctcactctat
     5881 cctgaaggag atccccgcgg tgccggtctg ctacgcttga cggccggggc ctatcccgag
     5941 agcatcgccg tcgcgctctc cgaaagtgtt gccggtgaga tgctgcgttt gatggacgag
     6001 cgtcccaccg tagatttcgc gctggcgacg gtggcgcgga cgctagggtt gccggctggc
     6061 ggggctgtcg cgctcttcgc cctcggcagg acagtaggct ggatcggcca cgcgatagag
     6121 cagtatcgga gcggctccct catacgtccc agggcccgct acgtcggaga acagccgagc
     6181 cgacagcccg ataagcgcta aaggggtttg cttgtaggga cggttcgcga accgccctag
     6241 atctgaatat tcagaacgtc aagcccgctc aggatgatca gcacggcggc gacggagttg
     6301 aacgtcgcgt gggcgacgaa cgagggaagg aggcttcccg tccgctggaa gagtgcgcac
     6361 agggttatcg caaggatcaa gaggacgggc agaaggaccg gttccaggtg cagcagggcg
     6421 aacaggacgg aagacgcgag cgcggaagag ataaagatca tcctgccagg acgcctcctg
     6481 gaatgctccg ccccaacgga cctggcgtag ataagggccc ccagccggta gagaccgttg
     6541 aagacgacac ccctgaatac cagttcctcg acggccggac cgaagagtac gacgatcagg
     6601 attatcgccg gtatggccac gccagggctt tcttgcaccc atccgacgag tccttgcata
     6661 aatggctgtt ggacggtcga ttccgtagag tagccgagcc gttccaggac gaagatgctg
     6721 atcgggttca cgatcatgcc gaggagtacg gcacctacgc cgacggccat ccccgcgcag
     6781 atccccgcca gaagtcctcc cctgggccgt gagaatccaa ggggcgccag cgagtatcct
     6841 accttcttgc ccaggctccg catccaaaat acggccagcc agaatccccc caccacggcc
     6901 cctgctaccc ccgcgcccat cacaacgaac gagagtaacg cgtcctgaga gtcaaccatc
     6961 tgtgcttcct acgtagactc tatccgggtc ccatccgtcg ccggcgtccc tcagtaacct
     7021 ttcctttatg gagcctggca gaagctcgat ttcctgcagc tcctcgacgc tcacccagcg
     7081 tacctctctg agggtcggct cggtaccag
//
LOCUS       DATMVF010000156        10912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_613213, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000156 DATMVF010000000
VERSION     DATMVF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613213"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1121)
                     /gene="murI"
                     /locus_tag="VN178_07765"
     CDS             complement(309..1121)
                     /gene="murI"
                     /locus_tag="VN178_07765"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWS80908.1"
                     /translation="MSDQRPIGVFDSGVGGLTVLAEIRDRLPYEHTIYFGDTARVPYG
                     VRDLAEVRWFTFEIVNYLIGMDVKLIVIACNTATASALRATQRSFEVPIIGVIEPGAR
                     AAVEETRNRRVGVLATPLTAASGAYRRAVHSLDAGVEVHQQACPEFVPMIECGVVDGP
                     ELETVARDYLQPIMERAVDAVVLGCTHYPLISAPINRILGPEVRLISSAGQTALEVGR
                     VLSRRDRLRRPRHIDDEGKATFVCSADPEEFAALGSRFLDREIEAVVPATVI"
     gene            complement(1338..1692)
                     /gene="ssrA"
                     /locus_tag="VN178_07770"
     tmRNA           complement(1338..1692)
                     /gene="ssrA"
                     /locus_tag="VN178_07770"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1982..2431)
                     /gene="smpB"
                     /locus_tag="VN178_07775"
     CDS             complement(1982..2431)
                     /gene="smpB"
                     /locus_tag="VN178_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWS80909.1"
                     /translation="MTDFARNKKALHDFAIEETYEAGIQLTGPEVKSVREGRANLKES
                     YARVRDGEVFLIGAHISPYANARQERQLPTRDRKLLLHRKEIDRLIGKSQEEGKTLIP
                     LKLYSKNGIVKLQIAVASRKRQYDKRREIAKKTADREIERAMKERLR"
     gene            complement(2428..4041)
                     /locus_tag="VN178_07780"
     CDS             complement(2428..4041)
                     /locus_tag="VN178_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HWS80910.1"
                     /translation="MILPAMLVRRGKYSVADPLFVEERRPVLVARKLRRGAEAGDIVL
                     VNAREKNRSLRGEVTRVIGPSEDPRNVYEALFASIGTSREFPRKVDEGAEAVAHREMG
                     ERRDLRHLPTVTIDGADAKDFDDAISVERSADGYRVWVHIADVTHYVEPGSVLDRQAA
                     YRGNSVYLPGTVAPMLPGRLSNDVCSLRPHSERAAVTVEMEVSGEGESKSSKAFRSMI
                     VSDARLTYEAVDAFLRGEETIGETDLVRTAYELSRRLRMRAARRGKLELGGREPEYTI
                     GEDGVPTGAGVRRSTPARELIEELMVLANESVARMLRAKKGGIFRVHERPDQDSLELL
                     GHRLAAVGMQAEPTPENLGTISQMAKSDAVNYLILRSIPRALYSPEDLGHYGLALEHY
                     THFTSPIRRYADVLVHRALLGDEPPEDLTGIANHISEREYRSMICERTADEYTLMWLM
                     RDRVGEVLEGTIVSTAAFGLFVELETGATGLVHVSKLPGWWELEPSGVVLSNDAIGAS
                     YRVGDRVLVELLDVLPLMQRAELRVVKRL"
     gene            complement(4200..5084)
                     /locus_tag="VN178_07785"
     CDS             complement(4200..5084)
                     /locus_tag="VN178_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWS80911.1"
                     /translation="MRVRPGFFLAEALKNLRLNPLMSITAVTTTFICILVFGLGLLVS
                     AHIQGIIDSVREDVSVEAFMPNSTRKDVVALVKQVEEWPEVANATRVSEEQAFAKFKD
                     KFEDQPDLYQDLQTGVFPPSVQIQLEDPDSAGAVAARLKNQGFTERNLSYPQQTIDRL
                     NSITSYVIWGLYGATLLFLVASVLLISNAIRLSIFARRKEIEVMKLVGASDGFVRTPF
                     VFEGLLQAFAGATLAAFAVIWINLLFVDWSHNALPYVPISSDALNAFSTLLVLIAVGV
                     AIGVIGSFLSVTRFLRKI"
     gene            complement(5074..5760)
                     /gene="ftsE"
                     /locus_tag="VN178_07790"
     CDS             complement(5074..5760)
                     /gene="ftsE"
                     /locus_tag="VN178_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWS80912.1"
                     /translation="MIRLDNVTKVYGKDTVALERVNLGIDSGEFVFLVGASGSGKSTI
                     VRLIIKEMEPSAGAIYVNGTRLSSVPRRRIPRLRRGIGCVFQDFKLLPTKTAAENVAY
                     AMEVTGQRRRTIRTKVPQILDLVGLSAKMDKFPEMLSGGEQQRVSIARAFVSRPPILV
                     ADEPTGNLDPTTSVSIMQLLHRINRLGTTVLVATHDREMVDVMRKRVVALEAGRVVRD
                     KVKGAYADEG"
     gene            complement(5837..6559)
                     /locus_tag="VN178_07795"
     CDS             complement(5837..6559)
                     /locus_tag="VN178_07795"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HWS80913.1"
                     /translation="MRSVVAAVLAPVLLVFLIGPYTFLPTLLEYAVARDVKARLGIEK
                     QPDVELKSEPPLRMLAGEFSGGRVVMKNTDLGNVTATRARIDLDPFDIDVWATMREGR
                     VVAREPLSGGIRVAVPESEVSRLAKAGSEVPIEGLDVQEGGVIARSEVSVLGRRFPAS
                     IEGKPALRDGELIFEPQGLTAAGVRVPDELADRLLAGTSFEYPLDRLPYQTTITSVEA
                     QEGRIILSGRVPSVPLGAYPGG"
     gene            complement(6564..7655)
                     /gene="prfB"
                     /locus_tag="VN178_07800"
     CDS             complement(6564..7655)
                     /gene="prfB"
                     /locus_tag="VN178_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWS80914.1"
                     /translation="MAELKYKTSELEGRLAELEAFFDVEGLRAEAEELGEEMSRPGFW
                     DDPEEARAVSARFSRVQGRVELLDGLKASLSDSGELLELAEEDGELLPEVREELRRVE
                     RVLEEQEVARLFAGEYDEGDAILTINPGAGGVDSQDWAEMLARMYQRWAERRGFGLEV
                     IEYTVGEEAGIKSATFTVSGEYAFGLLSAERGVHRLVRISPFDAGARRHTSFASVAVA
                     PVVGEAVEVEIEEKDLKVDTYRASGAGGQHVNKTDSAVRITHLPTGIVAQCQNERSQH
                     QNREVAMRVLRARLFELEREKREQEIAAQSGEKADIEWGSQIRSYVLHPYKLVKDHRT
                     GEETANVDRVLDGDLDAFVYAYLKNRTVA"
     gene            complement(7655..10240)
                     /gene="secA"
                     /locus_tag="VN178_07805"
     CDS             complement(7655..10240)
                     /gene="secA"
                     /locus_tag="VN178_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWS80915.1"
                     /translation="MANLLTKILRMGEGRKVKVLQRRVEAVSALEPEMEKLSDSQLRE
                     KTDEFRERLAGGETIDELLPEAFAVVREVSRRTLGMRPFDEQVMGGIVLHEGKIAEMK
                     TGEGKTLAATMPVYLNALEDKGVHVVTVNDYLARRDAAWMGEIYTFLGLEVGLIQESM
                     SFDERKVAYDSHVTYGTNAQFGFDYLRDNIATSTDQLVQRELNFGIVDEVDSILVDEA
                     RTPLIISGMPETAADVYYRFAAIVPRLKSGEDYEVDEKKQQVAPTESGVEKVEKALGI
                     ENLYDDVNTNLVNHLNQALRAQTLFHKDDEYIVTDGQVYIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVEIREENQTVATITIQNFFRMYDKLSGMTGTAATEADEFMHTYKMEVVS
                     IPTHEEMIRDDKDDLVYKTEKSKHKAVVEDISERNEMGQPILVGTVSVDVSERLSSLL
                     NRRGIAHNVLNAKQHEREAEIIAGAGERGAVTIATNMAGRGTDIKLGEGVSELGGLYV
                     LGTERHEARRIDNQLRGRSGRQGDPGESRFYLSFEDELLRLFGGQRMQGIIERIGLEE
                     DVPIEAGMISNSVRRAQEQVESRNFQMRKRILEYDDVLNKQREVIYAIRRDILMGEDV
                     DTQSYVEDVLTEVVSEYVSENVYPEDWDLEALTTEVNRFYPSRIDFKAIDVETLTAAE
                     VLAMVLQDADERLEERKAEWDERTAELEKRGLARGDGVATFEEAERRTLLSVVDSRWR
                     EHLYEMDYLREGIGWRGLSQRDPLVEYKREGFDIFQEMERGLKADYVTYIYRVENIKV
                     REEEMQQLSYSGGEEPNQRQKGPRKAAAKIGRNEPCPCGSGKKYKKCHGQPGAPPLAS
                     EAQAG"
     gene            complement(10274..10846)
                     /gene="raiA"
                     /locus_tag="VN178_07810"
     CDS             complement(10274..10846)
                     /gene="raiA"
                     /locus_tag="VN178_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWS80916.1"
                     /translation="MDILIKGRNIPVTEALERYAWEKVERVTRFFDDERTASRAEVEL
                     IHERNRAVSEPEVAEATLFINGSVLKASESSEDMYASIDGMSDKLERQVKRFRGRQID
                     RWQGQLKNTPDVAPTGAQPFIVEEEEEIEPRIVRTKQFQMKPMGAEEAVLQLELLDHD
                     FYVFTSADTGDINVVYRRRDGDYGLIEPAR"
BASE COUNT         1781 a         3576 c         3350 g         2205 t
ORIGIN      
        1 ctgctcccaa agcaggtgcg ctaccagact gcgccacgcc ccgccgagac cgggcatgag
       61 tatagcaaac agccggtcag cacggcgctt cgcgccttgt cagcacgctc aggcttttag
      121 ccttcgcttg tcagcatttc agcacgtcgt ctcggggcgc agccatcggc gtagaaccag
      181 accgccactc aaagcggtgc ctcgtgcgct accgggcgag cccagaggac agaagctgac
      241 cgctgagagc gaggcccgaa gggccgaagc gtgctgacaa gcgaccgaag ggagcgtgct
      301 gacttgctct agattacggt cgccgggact accgcctcta tctcccggtc caggaagcgg
      361 cttccgaggg cggcaaactc ttcagggtct gcgctgcaca cgaaggtggc tttaccttcg
      421 tcgtctatgt ggcgcgggcg gcgcaagcgg tcgcggcggg agaggacgcg accgacctcc
      481 agggcggtct ggcccgcgga ggagatcaga cgcacctcag gacccaggat gcggttgata
      541 ggcgccgaga tgagcgggta gtgggtgcac cccagcacga cggcgtcgac ggcacgctcc
      601 atgatgggct ggagatagtc ccgcgcgacc gtttcgagct ctgggccatc gacgacgccg
      661 cattcgatca tgggcacgaa ctcggggcag gcctgctggt ggacctcgac ccccgcgtcc
      721 agggagtgga cggcacgcct gtaggcgccg ctcgccgcgg tcagcggcgt cgcaagcacg
      781 ccgacgcgcc tgttgcgcgt ctcctccacc gcggcccgcg cccccggctc tatgacgcct
      841 atgatcggta cttcgaagga ccgctgcgtc gccctcaggg ccgaagcggt cgcggtattg
      901 cacgcgatca cgatcagctt cacgtccatg cctatgaggt agttgacgat ctcgaaagtg
      961 aaccagcgga cttcggcgag gtcccttacc ccgtatggaa ccctcgccgt atccccgaaa
     1021 tagatggtgt gttcgtaagg gagacggtcc ctgatctccg cgagcaccgt cagcccgccg
     1081 acacccgaat caaagactcc aatgggccgt tgatcgctca cgggagtgca ttatatccgg
     1141 ttagcacgct gccttcggca gcttgtcagc acgctcaggc ttttagcctt cgcttgtcag
     1201 catttcagcg agtcgttccg tggcccccac gagcgtgaaa cccggacgct tcgggtagcg
     1261 gtccgttgtg cacggcaagg tgaagcccga agcgccgtgc tggctagcgc gcttcgcggg
     1321 ttccccgcga agcgctagag tggaggcggc gggattcgaa cccgcgtccg cgagcgcgcc
     1381 gtccgtgaga ttctacgagc gtagctccca gtctagcttc gcctgccgcg acggaccggg
     1441 gcgccgccgc gaggctagcc ctttgagtgt cccctcagcg ctccgggcca gaaccccggg
     1501 ggtaagccca ctaaacgaca ccgggaaccg ggccgtgggc cgcccggacc gatgggcatc
     1561 gctatttaag cggctgccag ctcaacatta tcgttggcgt gtatctttcg ctgccggcgt
     1621 tttacgagtc tccagcaaac tcggctcgtc tcacaggacg ctgaccactc ccgtcgaagc
     1681 ctatgcgccc ccttatacgg cagatattct accattctgg ggagaatgtt aaagctggtc
     1741 agcacgcatc gggcctttcg gccctcgctt gtcagcatgt cagattttcc ctttctggat
     1801 cttgccctgg cgcgcacgac gaaccgcttc cagtggcggt cggggcttac ccacttggaa
     1861 agatcatggg acgacctggc tgaaatgctg acgagccgcc gagcggagcg aggcggcggg
     1921 ctgacaagcg aaggcgtaag cctgagcgtg ctgactagcg cgatttgctt cgcaaatcgc
     1981 tttagcggag gcgttctttc atggcgcgtt cgatctcgcg gtccgccgtc ttcttggcga
     2041 tctcacgccg cttgtcgtac tggcgcttgc ggctcgcgac ggcgatctgg agcttgacta
     2101 tgccgttttt ggagtagagc tttaggggga tgagggtctt cccttcctcc tggctcttgc
     2161 cgatgaggcg gtcgatctcc tttctgtgca gcagcagctt gcggtcgcgg gtggggagtt
     2221 gccgttcctg cctggcgttc gcgtaaggcg agatgtgcgc cccgatcagg aatacttcgc
     2281 cgtccctcac cctggcatag ctctccttga ggttggccct gccctccctg acggacttta
     2341 cttcgggccc cgtcagctgg atgccggcct cgtaggtctc ctcgatggca aagtcgtgga
     2401 gggccttctt gtttcgcgcg aagtctgtca caaccttttc accactctca gttcggcccg
     2461 ctgcatcagc gggagaacgt caagcaactc tactaagacc ctgtccccca ccctgtagga
     2521 agcgcctatg gcgtcgttgg agaggacgac gccgctcggc tcgagctccc accatccggg
     2581 gagtttgctc acgtggacga ggcctgtggc acccgtctcg agctcgacga agaggccgaa
     2641 ggctgcggtg cttacgatgg tcccttctag cacctcccca acacggtctc gcatcagcca
     2701 catgagcgtg tactcgtccg cggtgcgttc gcagatcatg gatctgtact cccgctcgga
     2761 gatgtggttc gcgataccgg tgaggtcttc gggcggctcg tctcccagca gggcgcggtg
     2821 gacgaggacg tcggcgtagc gccggatcgg ggaggtgaag tgcgtgtagt gctccagcgc
     2881 aagtccgtag tgtccgaggt cctcgggaga atacagggcg cgaggaatgg agcgtaggat
     2941 caggtagttt accgcgtccg acttggccat ctgggagatc gtcccgaggt tctcgggcgt
     3001 cggctcggcc tgcataccga cggcggcgag cctatggcca aggagctcca gagaatcctg
     3061 gtcgggcctc tcgtgcaccc tgaagatgcc gcccttcttt gcgcgcaaca ttctcgcgac
     3121 cgactcgttg gcgaggacca tgagctcctc gatcaactcc ctggccggcg tggaacggcg
     3181 aactccggcg cccgtgggta ccccatcctc gccgatcgtg tactctggct cccgccctcc
     3241 cagctccagc ttcccacgcc tcgctgcccg catcctgagc ctgcgcgaga gttcgtacgc
     3301 ggtccttacc agatccgtct cacctattgt ctcttcaccg cgcagaaacg cgtcgacggc
     3361 ctcataggtg agcctggcat cgctgacgat catgctccgg aaagccttgg agctcttcga
     3421 ctcgccttcc ccggagacct ccatctctac cgttacggcg gcccgctccg agtgcgggcg
     3481 cagggagcag acgtcgttgg agagacgccc gggaagcatc ggcgcgacgg tccctgggag
     3541 gtacacggag ttgcccctgt aggcggcctg ccggtcgagg accgagccag gctcgacgta
     3601 atgggttacg tcggctatgt ggacccatac cctgtagccg tctgcggaac gctcgaccga
     3661 gatcgcatcg tcgaagtcct tcgcgtcggc gccgtctatg gtcaccgtgg ggaggtgacg
     3721 caggtccctg cgctccccca tctccctgtg cgcgacggcc tcggccccct catcgacctt
     3781 tctcggaaac tcgcgtgagg ttccgatcga ggcgaagagc gcctcataga cgttgcgcgg
     3841 gtcctcagaa ggcccgataa cgcgggttac ttctcccctg agcgagcggt tcttctcgcg
     3901 ggcgttcacg agaactatgt cgccggcctc agcacccctg cgaagcttgc gtgcgaccag
     3961 cacaggacgc cgctcttcga cgaagagcgg gtctgctaca gagtacttcc cccgacggac
     4021 cagcatagcc ggaagtatca agagggcgct ccgatcagag gtgtctggca aagcacaccg
     4081 tgagtctacc atgcggctcc gccgcgcaat cagctgtcag ctttcagcgg tcagcttttt
     4141 gttcttgctg atggctgatg gctaatggct gaaagctgaa gatatagcgc cctagggcgc
     4201 tatatcttcc tcaagaatct tgttacggag aggaagctgc cgattacgcc gatggccact
     4261 cctacggcta tgaggacgag cagggtcgag aaggcgttta gtgcgtcgct ggagatcggt
     4321 acgtagggga gggcattgtg cgaccagtcg acgaagagaa ggttgatcca gatcacggcg
     4381 aacgccgcga gtgtcgctcc ggcgaaggcc tggaggagtc cttcgaagac gaatggggtg
     4441 cgcacgaagc cgtcggaggc gccgactagt ttcatcacct caatctcctt gcgcctggcg
     4501 aagatggaga gtctgatggc gttggagata aggagtacgc tggcgacaag gaacaggagc
     4561 gtggccccgt agaggcccca gatcacgtac gaagttatcg agttgagacg gtcgatggtc
     4621 tgctgggggt agctcaggtt tctctctgtg aacccttggt tcttcagcct cgcggcaacg
     4681 gcgccggcgc tatccgggtc ctcaagctga atctggacgg aaggagggaa gacgcccgtt
     4741 tgcaggtcct ggtacagatc gggctggtcc tcgaacttat ccttgaactt ggcgaacgcc
     4801 tgctcctcgg agacgcgggt cgcattggcg acctcgggcc actcttcgac ttgtttcacg
     4861 agggcgacta cgtccttgcg cgtggagttc ggcatgaatg cctcgacgct gacgtcctcg
     4921 cgcacagagt cgattatgcc ctggatgtgg gcgctgacga ggaggccgag cccgaagacg
     4981 aggatgcaga tgaacgtggt ggtgacggcg gtgatgctca tgagcggatt caggcggagg
     5041 ttcttgaggg cctcggccag aaagaacccc ggtctaaccc tcatcggcgt aagccccctt
     5101 gaccttgtcg cgtacgaccc gccccgcctc cagggcgacc acccgcttgc gcattacgtc
     5161 caccatctcc ctatcgtggg tcgcgaccag gacggtggtg cccaggcggt tgatcctgtg
     5221 caggagctgc atgatactga cgctggttgt ggggtccagg ttgcccgtcg gctcgtcggc
     5281 gacgaggatg ggcggccgcg acacgaaggc cctggctatg gagacgcgct gttgctcgcc
     5341 acccgagagc atttcgggaa acttgtccat cttcgccgag aggcccacga ggtcgagtat
     5401 ctggggcacc ttcgtgcgga tggtacgccg cctctggccc gtcacttcca tcgcgtaggc
     5461 gacgttctcg gccgcggtct tggtcgggag cagtttgaaa tcctgaaaga cgcatcctat
     5521 tccccggcgc agacgaggga tcctgcgccg cggcaccgag gagagcctgg tgccgttcac
     5581 gtagatggcc cccgccgagg gctccatctc cttgatgatc aggcgcacta tggtcgactt
     5641 gccagacccg ctggctccca ccaggaacac gaactcgccc gaatctatcc cgaggttcac
     5701 ccgctccagg gcgacggtat ctttgccata cactttggtg acattatcta gtcggatcat
     5761 tacttctcca cgcgcgattc ggagtccccc tccagggccc ggccatcgga gacgataaca
     5821 acaacccagg gcgttatcaa ccgccggggt aagcgccgag cggcacgctc ggcactctgc
     5881 cgctgagaat gatccgccct tcctgggcct ccacgctggt gatggtggtc tggtaaggca
     5941 gacggtccag ggggtactcg aaggacgtgc cggcgagcaa ccggtctgcg agctcatcgg
     6001 gcacccgaac tcccgccgcc gtgaggccct gcggctcgaa gatcagctct ccgtcgcgca
     6061 gggcgggctt cccttctatg gaggccggga accgcctgcc gagcaccgag acctccgacc
     6121 tggcgatcac cccgccctcc tgcacgtcca gtccctctat gggtacctcg gaacccgcct
     6181 tcgccaggcg cgagacttcg ctctcgggca cggcgacccg tatacctccc gacaggggct
     6241 ccctggccac cacgcgaccc tctctcatgg tggcccagac gtcgatgtcg aaggggtcga
     6301 ggtcgatacg cgccctcgtt gccgtgacgt tgcccagatc ggtattcttc atgacgacgc
     6361 gtcctcccga gaactcgcca gccagcatgc gcagcggggg ctcgctcttc agctctacat
     6421 cgggctgctt ttcgatcccg agccgggcct tgacgtccct ggcgaccgcg tactccagca
     6481 gcgtgggcaa gaacgtgtac ggtccgatca ggaagacgag gagtacgggg gcgagcacgg
     6541 ccgcaacgac gctccgcatg gcgctaggcc acggtcctgt tcttcaagta agcatagacg
     6601 aaggcatcga ggtcaccgtc gagcacgcgg tccacgttcg ccgtctcctc gccggtgcga
     6661 tggtccttca cgagcttgta agggtggagt acgtaggacc ggatctgcga accccactcg
     6721 atatccgcct tctccccgct ttgtgcggcg atctcctgct cccgcttctc ccgctcgagt
     6781 tcgaagagcc gcgccctgag gacccgcatg gccacctcgc ggttctggtg ctggctgcgc
     6841 tcgttctggc actgcgccac gatgcccgtc gggaggtgcg tgatcctgac ggccgaatcg
     6901 gtcttgttga cgtgctggcc gccggccccc gaggccctgt aggtatccac cttgagatct
     6961 ttctcctcga tctcgacctc gacggcctca cctacgacgg gtgccacggc gacggaggcg
     7021 aagctggtgt gcctcctcgc gccggcgtcg aaagggctga tcctgacgag acgatgcacc
     7081 ccgcgctcgg cggataagag cccgaaggcg tactccccgg agacggtgaa ggtggcgctt
     7141 tttatgcccg cctcctcgcc tacggtgtac tcgatcacct cgagcccgaa gccccgccgc
     7201 tcagcccagc gctggtacat tcgcgcgagc atctcggccc agtcctgcga atccaccccg
     7261 cccgcgcccg ggtttatggt gagtatggcg tcgccctcgt cgtactcgcc ggcgaagagc
     7321 ctggcaacct cttgctcctc gagcacccgc tctacccgcc tgagctcctc cctgacctcg
     7381 gggagcaact ctccgtcctc ttcggcgagc tcgagtagtt cccccgagtc cgagaggctc
     7441 gctttgagcc cgtcgagcag ctcgaccctg ccctgcaccc gcgagaagcg tgccgacacc
     7501 gccctggcct cttcgggatc atcccagaaa ccgggtcggc tcatttcctc gccgagctct
     7561 tcggcctccg cacgcaaccc ttccacgtcg aagaacgcct cgagctcggc gaggcgtcct
     7621 tccagctcgg acgttttgta cttgagctcg gccactaacc tgcctgggct tcggacgcga
     7681 gcggcggcgc gcccggctgt ccgtgacact tcttgtactt cttcccgctg ccgcacggac
     7741 agggctcgtt gcgcccgatc ttggccgctg ccttgcgcgg gcccttctgg cgctggttcg
     7801 gctcttcgcc gccgctgtag gagagctgct gcatctcctc ctcgcgcacc ttgatgttct
     7861 ccacacggta gatgtaggtg acgtagtccg ccttcaggcc ccgctccatc tcctggaaga
     7921 tgtcgaagcc ctctcgcttg tactcgacca gcggatcgcg ctgggagagc ccgcgccagc
     7981 cgatcccctc gcgcaggtag tccatctcgt agaggtgctc cctccagcgg gaatcgacca
     8041 cagagagcag cgtcctacgc tcggcctcct cgaaagtggc caccccgtcg ccgcgggcta
     8101 gcccgcgctt ctcgagctcg gcggtccgct cgtcccactc ggccttgcgc tcctcgaggc
     8161 gctcgtcggc gtcctgcagg accatcgcga ggacctcggc ggccgtaagg gtctcgacgt
     8221 cgatggcttt gaaatcgatc ctgctgggat agaagcggtt tacctcggtc gtgagggctt
     8281 cgaggtccca gtcttcgggg tagacgttct cggagacgta ttcggagacg acttccgtaa
     8341 gcacgtcctc cacgtaggac tgcgtgtcga cgtcctcccc catcaggatg tcacgtctta
     8401 tcgcgtagat cacctcgcgc tgcttgttca agacgtcgtc gtactcgagt atgcgcttgc
     8461 gcatctggaa gttgcggctc tcgacctgct cctgggcgcg cctcacggag ttggagatca
     8521 taccggcctc gatcggcacg tcttcttcga gccctatgcg ctcgatgatc ccctgcatcc
     8581 gctgtccgcc gaagagcctg aggagctcgt cctcgaagga caggtagaac ctggactcgc
     8641 cgggatcccc ctgcctcccc gagcggcccc tgagctggtt gtctatgcgg cgcgcctcgt
     8701 gacgctcggt gccgaggacg tagaggcctc caagctcgga cactccctcg ccgagcttga
     8761 tgtccgtacc acggcccgcc atgttcgtcg ctatggtcac ggcgccccgc tcgcccgccc
     8821 cggcgatgat ctccgcctcc cgctcgtgct gcttggcgtt gagtacgttg tgcgcgatcc
     8881 cacgccggtt caacagcgac gagaggcgct cggagacgtc cacggagacc gtgccgacga
     8941 ggatgggttg gcccatctcg ttccgctccg agatgtcctc gaccacggct ttgtgctttg
     9001 acttctcggt cttgtagacg aggtcgtctt tgtcgtccct gatcatctcc tcgtgcgtcg
     9061 ggatggagac gacttccatc ttgtaggtgt gcatgaactc gtcggcctcg gtcgccgccg
     9121 tgcccgtcat gccggagagc ttgtcgtaca tcctgaagaa gttctggatg gtgatggtgg
     9181 ctacggtctg gttctcttcc ctgatctcga cgccctcttt ggcctcgatg gcctggtgga
     9241 gcccctcgga gtaacgcctg ccctcgagga ctcgccccgt gaactcgtcg actatgtaga
     9301 cctgcccgtc ggtgacgatg tactcgtcgt ccttgtggaa gagagtctgc gccctgagcg
     9361 cctggttcag gtggttgacg aggttggtgt tgacgtcgtc gtagagattc tctatgccca
     9421 gggccttctc gaccttctct acgccgctct cggtcggggc tacctgctgc tttttctcat
     9481 ccacctcgta gtcttcgccg ctcttgaggc gcgggactat ggcggcgaag cggtagtaga
     9541 cgtctgcggc ggtctcaggc atacccgaga tgatgagcgg agtgcgggcc tcgtcgacca
     9601 ggatggagtc gacctcgtcc actatcccga agttgagctc ccgctgcacg agctggtccg
     9661 tggaggtcgc tatgttgtcc ctgaggtaat cgaagccgaa ctgcgcgttc gtgccgtatg
     9721 tgacatgcga gtcgtaggcg accttccgct cgtcgaagct catgctctcc tggataaggc
     9781 cgacctctag ccccaggaaa gtgtagatct cacccatcca ggccgcgtcg cggcgggcca
     9841 gatagtcgtt gacggtgacg acgtggacgc ctttgtcttc cagcgcgttg aggtagacgg
     9901 gcatggtcgc cgcgagcgtc ttgccctcgc ccgtcttcat ctcggcgatc ttcccttcgt
     9961 gcaagactat gccgcccatt acctgctcat cgaagggacg catccccagg gtccgcctgg
    10021 agacctcccg cacgactgcg aaggcctcgg gcagcaactc gtcgatggtc tcccccccgg
    10081 cgagcctctc gcgaaactcg tccgtcttct cccgcagttg cgaatcggag agcttctcca
    10141 tctcgggctc caacgccgag accgcctcca cgcgccgctg gaggaccttg accttacggc
    10201 cctcgcccat cctcaaaatc ttcgtcaaca agttcgccaa aacaagctac ctcgtagtag
    10261 tagtcgtacc gggctaacgg gcaggctcta tgagcccgta gtctccgtca cgacgccggt
    10321 ataccacatt tatatcaccg gtatcggcgc tcgtgaacac gtagaagtcg tggtcgagga
    10381 gctccagttg gaggacggcc tcctcggcgc ccatcggctt catctggaac tgcttggtcc
    10441 tcactatacg gggttcgatc tcctcttctt cctcgacgat gaagggttgg gcgcctgtgg
    10501 gggccacgtc cggcgtgttc ttcagctggc cctgccagcg gtcgatctgg cgccccctga
    10561 agcgcttgac ctgccgctcg agtttgtccg acatcccatc tatggaggcg tacatgtctt
    10621 ctgaggactc actggccttc agcacggacc cgttgataaa caacgtggcc tcggccacct
    10681 ctggctcgga gacggcgcgg ttgcgctcgt ggatgagctc cacctcggcc cgcgaggctg
    10741 tgcgctcatc atcgaagaac ctggtgaccc gctccacctt ctcccaggcg tagcgctcca
    10801 gagcctccgt cacagggatg ttgcggcctt tgatgaggat gtccatgtcg ctcctccctt
    10861 cggtcggcat tagccgtcag ctatcagcgg tcagctttca gctttgccgc tc
//
LOCUS       DATMVF010000157        28846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_299631, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000157 DATMVF010000000
VERSION     DATMVF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 28846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28846
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299631"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VN178_07815"
     CDS             complement(<1..171)
                     /locus_tag="VN178_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80917.1"
                     /translation="MRARQEEGRDGLNGALLVAGTHSDAGKSVVVAGICRWLAREGAS
                     VAPFKAQNMALNS"
     gene            complement(135..866)
                     /locus_tag="VN178_07820"
     CDS             complement(135..866)
                     /locus_tag="VN178_07820"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HWS80918.1"
                     /translation="MVSAYLRRGMAAGLLAGLFAGFFAFFVGEPVLDRAIALEESAHA
                     HEGGGHVHGGGGEEIFGRTTQKVGLFFATGLFGVTVGGVFGIGYAFFRGRLAAGSDLK
                     RSISLAGAIFAGAFLIPFLKYPANPPSVGDPSTIRERTAAYFSLVALSLLAIFLAWLA
                     ARTLEARGVDALRRRLVVGAGLVVVVGVLFLFLPAAPSTGGFPSGTLWAFRLSSFGTQ
                     LAFWAGLGLLFGVLCERARRKGVTA"
     gene            complement(875..1123)
                     /locus_tag="VN178_07825"
     CDS             complement(875..1123)
                     /locus_tag="VN178_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80919.1"
                     /translation="MIPNRGEDPEKEASVEKTFGRRHPTLPLWSLLALALLLLALFVL
                     LSASGALLAPLLGQAAGTFDYLHEFAHDGRHLLGVPCH"
     regulatory      complement(1113..1280)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1452..2117
                     /locus_tag="VN178_07830"
     CDS             1452..2117
                     /locus_tag="VN178_07830"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWS80920.1"
                     /translation="MESLLDFIRSIPDSPSAVLGFVTNLLVTHGYLVILFGAALDNFG
                     LPASGDIVLFAGGLFANGGQLALPLVMLSGFAGAFISDNSVYWIGRIGGRPMIYRILK
                     MRFIHFLVSERSLARVERYFEAHGGKTVFVGRFGPGLRSMTPLFAGVTRMRYYRFLPY
                     NLAAAVVWAVAYSLVGYVFGQYWEELLATAKSLGYGLIGIVALLVLAYVLRRRRFRNN
                     LDN"
     gene            complement(2174..2455)
                     /locus_tag="VN178_07835"
     CDS             complement(2174..2455)
                     /locus_tag="VN178_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80921.1"
                     /translation="MMETDLAGQLEGLFHEVGAAHHQAYIETDGADPEWPLWYADYLR
                     ERLGELLDASFTKSELVHMLVLVANEQPLVAPGANWARYYAKKFIALYT"
     gene            complement(2731..3423)
                     /locus_tag="VN178_07840"
     CDS             complement(2731..3423)
                     /locus_tag="VN178_07840"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HWS80922.1"
                     /translation="MRKAILLLATMALAILLAAGAAWAITYGQPDGDRHPYVGAMVVA
                     DEQGEFLFCSGTLIAPDVFLTAAHCMAAIDQFGLEFRGVTFDSVFDPDTSVVYPGTPH
                     AHPDFPGPSSDAKDIAAVVLDEEVSGIQPALLPGAGLLDRLAEDGALKGQSFTAVGYG
                     ATERTHEPGSGAPVFGEDGTRMYAVSTFSALNKNFLRLSQNPSTGDAGTCYGDSGGRT
                     SLATHASSRPSR"
     gene            complement(4009..5193)
                     /locus_tag="VN178_07845"
     CDS             complement(4009..5193)
                     /locus_tag="VN178_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80923.1"
                     /translation="MRKSETMRAASRLLLSHPIFCLLAVLAAVVVLGVFGPLKVVHAA
                     DVAVEGENFDVKPTGTSVVTGTMYSNGKALRFSNNTAIAKEPLTFNSSGDVVLMARAT
                     QSGGSPKLRVSVNGTFTAPAQAITNSGAPRAYTFDVNAPSGNDVQISVKAANTGSGRK
                     PFLDYVTFPPSGGGGSLTYIGSATGDTDTTPQGKVTVPVPPGTEQGDFMLALLQADYG
                     NVGGTPPSGWKLIDEHLEGRDLSLHAYYRIAAATEPRSYTWNVVNTTLNHDPLAGGTI
                     LTFRGVDQASPVFAKAVNPETADPAKINCPSVNAPNGGMLVCGFAHDDPQPDIKAPAG
                     MTRVSNFVIGNDDAHAVAYERIAEAGATGLRSATINPNLKGGKNDISMAISLRPSAVN
                     PP"
     gene            5422..6609
                     /locus_tag="VN178_07850"
     CDS             5422..6609
                     /locus_tag="VN178_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HWS80924.1"
                     /translation="MSGLNTREREDLERRNHELSVLNEIARELNRSVELGEALGFTLS
                     QVAELLGLRTAWIWLIDDSSPEPYLAAARNLPPALADDPRRMDGSGYCYCLDTYKKGD
                     LEGAANVNVLTCSRLDGLVDGTGGLRYHASIPLYAGEKKLGVMNVASPQWRGLSPEDL
                     QLLSTIGDLLAIAVERASLFDRSARLGAVEERNRLAREIHDTLAQGLTATGLQLESAE
                     ALLDDEFGAQRAREPLRRALSLTRSNLDEARRSVLDLRAAPLEGRSLPEALKTLVDRW
                     EAETDVATRFRAVNGSRPLPPRVEVALYRVCQEALTNIASHARASRVTVRLVATPQAV
                     RLTVKDDGHGFDPSSVADARHGIVGMRERVEILGGSLVIESVPGEGTRLDVTVPLEGI
                     RGG"
     gene            6602..7246
                     /locus_tag="VN178_07855"
     CDS             6602..7246
                     /locus_tag="VN178_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS80925.1"
                     /translation="MDKVRLLVADDHPMLREGLVAVLATQPDFEVVGEAADGDEVVRL
                     AESLDPDVILLDLEMPGTDGVEALEKLRDAGSSARTLVFTAYDTDARILGSLRAGARG
                     YLLKGASRQEIFGAIRTVQAGGSLLEPAVTDRLLERIEGEENVPQNRRLTSRELEVLA
                     LLARGLKNAEIAERLFISERTVKFHVGSILAKLEAQNRTEAARIAVRRGLVEAP"
     gene            7392..8120
                     /locus_tag="VN178_07860"
     CDS             7392..8120
                     /locus_tag="VN178_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS80926.1"
                     /translation="MTGESSDRTAIITGASRGLGLALARTLAGEGWTLIIDARGKEAL
                     ESARAELSRDARVIAIPGDVADPEHREDLAEAAREAGGVDALVNNASVLGPSPQPELL
                     DYPLDVFEQVYRNNVVAPLALIQTLHGELKPQARIINVTSDAAVEPYEGWGGYGSSKA
                     ALEQLSNILAAENPGLRVYRVDPGDMRTQMHQEAFPDEDISDRPLPEESVPGLLELLT
                     GDLPSGRYEASVLAARVGTTTAAS"
     gene            8136..9290
                     /locus_tag="VN178_07865"
     CDS             8136..9290
                     /locus_tag="VN178_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HWS80927.1"
                     /translation="MSHTLTEALESLDTFPDYPEARSPLRFELPRELEAGEPPEARGL
                     RRDEVRLMVSYLDDDRIVHSRFGDLPEFLRAGDTLVVNTSGTMNAALPAERSDGTPLT
                     VHLSTHLPADLWVVELRSSSGHEPVLDGKSGEVLSLPAGASMVLHTPYLSERRQRDDE
                     SNRLWISTLKLPVNLNDYLDLNGSPIRYGYVRESWPASYYQTVYATEVGSAEMPSAGR
                     AFTPELITRLVANGVSVVPLILHTGVASLEDDEPPYEEFYRVPPETAAAINSARAAGR
                     RVVAVGTTVVRALETVTDRDGSIHPGEGWTDVFITPERGIRSVDAMLTGLHEPRSTHL
                     SMLEALVGSVSEHPISTSGAEHLEVAYSEALKRGYLWHEFGDLHLILPGH"
     gene            complement(9423..9665)
                     /locus_tag="VN178_07870"
     CDS             complement(9423..9665)
                     /locus_tag="VN178_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80928.1"
                     /translation="MIVTSDDDSLPDGCRPRQVAGLVISFVNAFNSRDQASLSRIFAW
                     RMDMKADDERTAREAAGWLCTDPPGWKPGKAVVACT"
     gene            complement(10083..10709)
                     /locus_tag="VN178_07875"
     CDS             complement(10083..10709)
                     /locus_tag="VN178_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS80929.1"
                     /translation="MTPTRVFVVAPTPTMRAGLRSMLASVEAADLLVVGETGLSVGPV
                     PPLSGADVVLVADEELLEETALSVSEDGTLSLLLLSEDEDTASRLRTLSLRGWGVVPP
                     DAPSEELGAAVAAVAQGLTVLPRNLAERLLDEPAAAVEKLSEPLTARESEVLDLLGHG
                     LSNKMIARELHISEHTVKFHISSVYSKLGVSNRAEAVSQGARHGLISL"
     gene            complement(10706..11599)
                     /locus_tag="VN178_07880"
     CDS             complement(10706..11599)
                     /locus_tag="VN178_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWS80930.1"
                     /translation="MAELSGDSAALAGVRMIEAAQRSVVQVRSRGRGIGAGVIWPGDG
                     LVLTNHHVVSGRRSRRTVRVALHDGRTIDAEVLKSDRSLDLALLGLEGGQADLPAATV
                     GDSDALRVGELVYAIGHPWGSVGAVSAGIVGGVGELRGQGRSSSARYVRSDVALAPGN
                     SGGPLLNARGEVVAINAMIFGRMALSIPSNAAEAWIAEERRPRLGVGVLPVEVPVSQQ
                     REAGTAGLVIAGVETGGAAYRAGLLVGDVLLAAAGKPLGETQTLLDAVAHAGDVVSLR
                     VMRSGKIMVMEVRLEESGRAA"
     gene            complement(11611..12513)
                     /locus_tag="VN178_07885"
     CDS             complement(11611..12513)
                     /locus_tag="VN178_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWS80931.1"
                     /translation="MEHTDTLAALSEGMADAVENIAASVVKVNGRRRRSGSGVVFAQN
                     KVLTASHVLEREEDLSVETADGRTLTARFAGRDHSTDLAVLDVEGLDVEPATPAESEA
                     RVGQISLAVGSRSRGEGPRATLGVVSAVGGPVRTRRGPRLERYIQTDATPYPGFSGGP
                     LIDVRGNVLGILVSGWGRGAAFAIPADLAWRTAGSLSERGSVKRGYLGILSQPVRLPD
                     GQGLGLTQRGGLLVVGVEDGSPAGRGGLIVGDILATLDGQPVEDTDDLLVLLAGDRVG
                     SSVPVKLVRGGELAEVEITVGERG"
     gene            12766..14487
                     /gene="araD"
                     /locus_tag="VN178_07890"
     CDS             12766..14487
                     /gene="araD"
                     /locus_tag="VN178_07890"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HWS80932.1"
                     /translation="MNRKRRAEELRSHRWYGVRDLRSFGHRSRTKQMGYAAEDYSGKP
                     VVGIINTWSDINPCHTHFKERVEEVKRGVWQAGGFPVELPAMSLSEPFQKPSTMLYRN
                     FLAMETEELLRSHPIDGAVLMGGCDKTTPALLMGTITMDLPAIFVPAGPMLRGNWRGN
                     TLGSGSDVWKYWDEKRAGTIGDREWEEIEDGIARSPGHCMTMGTASTMTAVAEVLGMT
                     LPGASSIPAADSGHPRMASISGRRIVEMVWEDLKPSDILDDRSYEDAVTAVMALGGST
                     NAVIHLIALARRSGVPLDLARFDELSRRVPVLANIRPSGEYLMEDFYYAGGLRALLSR
                     LEEVLHLDRATAGGRTIGETLSSVYKDDVIRPFDDPLQEEGGLAVLRGNLAPDGCVIK
                     HSAADPKLLKHTGPAVAFEGYDDLARRIDDPDLKVDEDSVLVLRGAGPIGAPGMPEWG
                     LLPIPKKLLERGVRDMVRISDARMSGTAYGTCVLHVAPESYVGGPLALIEDGDEVELD
                     VPNRRLELRVDDRELSRRRAAWRQPQPHYGRGYGAIFARHATQANEGCDFDFLARGPL
                     VPEPKIH"
     gene            complement(14527..15222)
                     /locus_tag="VN178_07895"
     CDS             complement(14527..15222)
                     /locus_tag="VN178_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS80933.1"
                     /translation="MENSRIALVSGGNRGIGLEVCRQLPERGITVIMGSRDGEQGRDA
                     AAGLSGGVVVRQLDVADPESVERLSRSIEEEFGRLDILVNNAGISNDEGQSGADADLD
                     RVKESMEANLFGAWRLCRMAIPLMRRNGYGRIVNVSTGLAALEDMGGGSPGYRVSKTA
                     LNALTRILASELRGSGILVNAVNPGWVQTDMGGSGATRSVEEGAEALVWAATLPNSGP
                     TGGFFQDRRPVPW"
     gene            complement(15235..15696)
                     /locus_tag="VN178_07900"
     CDS             complement(15235..15696)
                     /locus_tag="VN178_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HWS80934.1"
                     /translation="MTGRVYVAVVGSGLATDEHYEKAREVGRLIVRRGGTVVCGGLSG
                     VMEAAARGATEAGGTAVGILPDEDRRRENEYLTHSVATGTGQARNLAVVCSGDVVIAV
                     GGEYGTLSEIGLARKVGRPVVVLEGWDLGEHVTLAPSPLAAVEAAFELIGR"
     gene            complement(15848..18130)
                     /locus_tag="VN178_07905"
     CDS             complement(15848..18130)
                     /locus_tag="VN178_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWS80935.1"
                     /translation="MTNMQVQSPLAGMSRRTVESLGDTVASLSPRDATLVKFPDLRPE
                     LSEALRKMGASRLYSHQLEAYERVLAGENVVVATATASGKSLCYKIPAFQNALGRAKS
                     RAVFLYPTKALAQDQLGKIKAFDLRGVHPATYDGDTPHALRADIRRRANILLTNPDML
                     NVGILPNHEAWGDFLRNLEIVAVDEAHVLRGVFGSHVAAVLRRLRRVAMLHGGDPRFV
                     LTSATIANPQELAESLTGMPFALVDNDGASTGERRVIFRNPPLLDKEKGERRSLLTEG
                     ALVFTNLVSQGVRTIAFARTRKAAELIYRYAADRLGVDGARRISPYRAGYTPRERREI
                     EGRLFRGDLLGVVSTNALELGVDVGALDAVICCGYPGSVASIWQQWGRAGRGKDPALA
                     VYIPGRDALDQFLFENPPRVLGRRVEAARVTMENPYILGPHLLAAAHEAPLDADDEEY
                     FGPAYRSVAKELMEARALASSGSRLVYVGGDNPARNISLRSASSETVLIADGEGELIG
                     TADATRAPSELHPGATYLHRGTAYEVESLDLQSHRAVARRVPNRYYTRPRVETDVEIL
                     EEVEERDLANGAVLHWGRVRTTDSVTFFKKVRVADDKEVGVYPLDLPDVVLETQALWV
                     TLPPLPREARPSFESFGGALHAGEHGLIGLLPLFAMCDRADIGGLSTPVHRQSRLPTV
                     FVYDGYPGGVGISRRGFDAFESLARDTLGVITRCPCERGCPACIQSPKCGNWNEPLSK
                     DGAVSLIRYILGQTSRYPTL"
     gene            complement(18203..18802)
                     /gene="recR"
                     /locus_tag="VN178_07910"
     CDS             complement(18203..18802)
                     /gene="recR"
                     /locus_tag="VN178_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWS80936.1"
                     /translation="MATYARPVERLITELSKLPSIGPRSAQRIAFHVIRGRKEDAIGL
                     AEALQEVKEKIKPCRRCFNLTEAEECDICRDARRDASVICVVEDPYDIGPIERTNEYR
                     GLYHVLGGALSPLDGVEPEDLRIAELLERVREEGTTELVIATNPNTTGEATALFIAQE
                     VKDLPVRVTALASGLPVGGDLEYADEVTLGRAFTGRREL"
     gene            complement(18810..19142)
                     /locus_tag="VN178_07915"
     CDS             complement(18810..19142)
                     /locus_tag="VN178_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWS80937.1"
                     /translation="MGNRRNVNKMMQQVQQMQQQMQQAQEELANETVTASAGGGAVRA
                     TMTGSMELVSIEIDPEVVDPEDIEMLQDMVLAAVNEAMTSSQELANKKLGGITGGLGD
                     LGLNIPGL"
     gene            complement(19410..21215)
                     /gene="dnaX"
                     /locus_tag="VN178_07920"
     CDS             complement(19410..21215)
                     /gene="dnaX"
                     /locus_tag="VN178_07920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWS80938.1"
                     /translation="MNHTTLYRRWRPRRFGDVVGQEPVVRTLRRAIETDRVSHAYLFS
                     GPRGTGKTSTAKVLAMGLNCEKGPTPEPDGTCESCRAIVGNSSMDVLEMDAASNRGID
                     EIRDLRDKVNLAPVAGRMKVYIIDEVHMLTAEAFNALLKMLEEPPEHVVFVLATTEKH
                     KVLPTIISRCQSFDFRRPSIETLVGKLDEIADAENIEVEPEALTVIAREGRGSFRDAE
                     GLLDQLSSFAEGRITASMVRELLGGVGPESLIETAYALHERRAADALRIVDRLSGGGK
                     DLGQFVGELLAHLRNLMLLPYAPEVALAGVGAEERPQLEEQANAIPTAGIMRLIEALG
                     DALSLMRRGGDPKLELELVFLKLTRDYTEPDLTTILDRLETLERAVENGSVATPPPRA
                     AADIVREESPAEVESAQETAEPDAPADDASILEDHDDLRESGIDLASDWHGIIGELKR
                     RRQALTAAVYGEARVESFDGSVLRLVYPEEQSFHVGMARDSGHLKKLGAVLGERLGSE
                     PHLEIRAAGGEASATIAAEEQPPTAAPIEQPAPTSEESPPKTVSQNGTDGTVAENSGP
                     GQGTVGRDDVIQDQREVFEIAREVGLFDENRSAKS"
     gene            complement(21483..21749)
                     /gene="ffs"
                     /locus_tag="VN178_07925"
     ncRNA           complement(21483..21749)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN178_07925"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(21976..23292)
                     /locus_tag="VN178_07930"
     CDS             complement(21976..23292)
                     /locus_tag="VN178_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HWS80939.1"
                     /translation="MARRTEEDMLVHQAKAVLDYNWTGEYTMPGPRLYPHQWSWDSAF
                     AAIGYSRYDQDRATRELRHLFEAQWKNGLLPQLVFNSRFTNYFPGPNFWHAKESPDAP
                     EHHETSGVVLPPIHAMAVLYIYRHAQDEAKAKDFLEYIFPRLGAWHDYLYRERDPDGE
                     GLVYIRHPWESGMDNSPMWDPILQRMHLRSDQIPRYHRADIHTVSASDRPTSDSYDRF
                     AYLVKYFADRDYDEAKIRQDCPFLVQDVLFNSLLSKAGRDLAEIARIVGEDPTPHEEL
                     AKKTKSAVEERLWDEDRGTYLDYDLVCGMPVPAYFGPNLAGPLYAGIAEPARAKRVID
                     TLENEGFGLADEAITPIPSYDVHGFGFSEERYWRGPVWINIDWFLMHGLEAYGYQDHA
                     QRLRKTIIELCKNEGFHEYFDPLTGRGLGSILFSWSAALLLDVLME"
     gene            23366..24499
                     /locus_tag="VN178_07935"
     CDS             23366..24499
                     /locus_tag="VN178_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWS80940.1"
                     /translation="MASNENSADILILSSAIGSGHMRASAAVVRGVDLLDPEKRCSIV
                     DFPHKVSPGVERLLRDAYLQALKLWPTAYGRLYESSGTGQQGTLVQQVQTAGLRTLER
                     LVSSTGAQALVAPHFYGAGVLGVYKKQNPQMFSAVVLTDYVPHPIGVPLNLDLYVVAD
                     DAAAEDVAKLGVPEERIHATGIPIDPIFEEPADPSPARKEVLNLDDDDLPVVLVMGGG
                     LGGGDLEESVMLLLQASEAMHLVVLCGSNERIRARLERLADRRGYEATFLAFTDRVRE
                     LMAGGTVLVTKPGGMSCTEALASRLPQVLYHPIPGQEEDNAAAMVRYGAGVMVRETSQ
                     ILGQTLKILTSPDHRHKMVEAAKTAHKPHSARSAASLILAGLP"
     gene            24496..25332
                     /locus_tag="VN178_07940"
     CDS             24496..25332
                     /locus_tag="VN178_07940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWS80941.1"
                     /translation="MRHPRNHVWKAAAATAAGGTALAALRTARGHPVRSASTLVSAGA
                     GMAAGGLMAYEWLTPRSWLYGPVFWHARTGERVVALTFDDGPCHPYTGQLLEILDREG
                     IRATFFQVGNNVIREPNLAAEVASRHEVGNHTFTHPHLTWSRPGTVREEMLRGQEAIH
                     EATGTLPRLFRVPHGWYGPQVISVAGELGMRCVGWSVMAWDWDRPPKDVIQRRILHGV
                     GPGGVTLLHDGQDTDAFPKADRSHTVAAVSDIISSLKANGYGFLTLPELMDLDAREHP
                     PG"
     gene            complement(25295..27418)
                     /locus_tag="VN178_07945"
     CDS             complement(25295..27418)
                     /locus_tag="VN178_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWS80942.1"
                     /translation="MRPKGSQTLDDWEAVPLAAPLGSVGEAARIKRAWTPVRVPWHWQ
                     LEEAFAAYEGLVLYRCRFAQGGLSEGAMLSLRFGGVYYAARVWLNGVYLGDHEGYFSA
                     FEFDVTDMVITNGENELLVEVFSPEESDENDRKTIGGVWARWDGMAPHINPGGIFKEV
                     SLVADGGVRIRSLAATADHTGRGVAHVDLYSRYKARVELAGRVRPLGFEAQGVEFERE
                     LQIEQGENCLEIGFRVPDVRVWSAWDRGEQALYELVLSCDGAEEQTRFGASTVELEDW
                     KVYLNGERIFLRGINYLPSDAYPARATKELLRKDASLVREAGMNAVRVHAHVSDGEFY
                     EACDELGLLVLQDFPLQWTHRRSILEPAVTQAGEMARILRGYPSVGIYLAHDEPFFVA
                     PPEKWSPLGLVRTAGEVLSPRWILWQRRVLDPAVVRAISEEDDSRPVIDAAGHPLTTN
                     HLYFGWYYGKFRNLERFVKVFPVFSRLPTEYGAQALPDPESLEEIWPSGTPPDWSVLS
                     LNYRLQIQRMARYVPWRGDRTLFLRESQRYQAEVLKHATELFRRRKYGPTGGTFAFML
                     NDPAPAVSWSVVDWRRKPKLAYETLFTAMSPVLFCAEYPKERYEVGTRISLPLFIIND
                     LLRELDEVGWDWELLLGGSSVARGTGETRVPKDSVMRIGEAVATLPAPGPAVLRLRLL
                     GDEGPLSNEYEFVVSQADARARRGP"
     gene            complement(27580..28365)
                     /gene="budA"
                     /locus_tag="VN178_07950"
     CDS             complement(27580..28365)
                     /gene="budA"
                     /locus_tag="VN178_07950"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HWS80943.1"
                     /translation="MPIDTSLVGALHVEYLRERDLRGNEHLHHTLFQTSTVDALLEGK
                     YDGDVSFAELGDRGDFGLGTFDALDGEMICLDGDFFQVKADGRAYAVDEDARTPFAVV
                     TLFEPDTYRTLPMTMDFEDLCDYVDGVIGGGTVCAAIRVDGRFEYVKTRSVPRQRKPY
                     PPLVEVVKDQPTFELHDVSGSLVGFRFPDYAQGLNVSGYHFHFITADRSAGGHVLGCK
                     LVGGELSIDHEGDLRLELPPEVGLPTPDQSAAKGETLDRIERE"
     gene            complement(28439..>28846)
                     /locus_tag="VN178_07955"
     CDS             complement(28439..>28846)
                     /locus_tag="VN178_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWS80944.1"
                     /translation="DELAALEAQAPAAAKGGTLLHLDLRADNLLLTPDDRVLVVDWPH
                     ARVGASWVDAVFFAPSVAMQGGPPPEELLAHHPHSRRADPDALTAVVAAVAGFFTSEG
                     LQPAPPGLPTLRPFQASQGEVAGGWLARRTGWR"
BASE COUNT         5207 a         9559 c         8829 g         5251 t
ORIGIN      
        1 ggagttgagg gccatgttct gggccttgaa cggcgcgacg ctcgcaccct cgcgagcgag
       61 ccagcggcag atgccggcca cgacgacgct cttccctgcg tcggagtggg ttcctgcgac
      121 gaggagggct ccgttcaagc cgtcacgccc ttcctcctgg cgcgctcgca cagcaccccg
      181 aagaggagtc cgagtccggc ccagaaggcc agctgggtgc cgaacgatga gagcctgaaa
      241 gcccagagcg tccccgacgg gaacccaccg gtggagggag cagcgggcaa gaacaagaac
      301 agcacgccaa cgacgacgac cagacccgcg ccgaccacaa gacgacgtcg caaggcgtcg
      361 acaccgcgtg cttcgagcgt ccttgcggcg agccaggcaa ggaagatggc gaggagcgag
      421 agggcgacga gcgagaagta ggccgcggtg cgctccctta tggtggaagg gtcgccgacg
      481 gatggcgggt tggccgggta cttgaggaag gggatcaaga acgcccccgc gaagatggcg
      541 ccggccaggg agatgctgcg cttgagatcg ctgcctgcgg ccagacgtcc gcggaagaat
      601 gcgtaaccga tgccgaacac cccacctacg gtcaccccga agagtcccgt ggcgaaaaac
      661 aagccgacct tctgggtcgt gcggccgaag atctcctcac ctcctccgcc gtgcacgtga
      721 cctccgccct cgtgggcatg ggccgattcc tccagcgcta tcgcgcggtc gaggacgggc
      781 tctccgacga agaaggcgaa gaagccggcg aagaggccgg cgagcagccc ggcggccatg
      841 ccgcggcgca ggtatgccga gaccacgggt tgccctagtg gcagggaacg ccgagcagat
      901 gtcgcccgtc gtgggcgaac tcgtgcaggt agtcgaaggt gcccgcggcc tgccccagga
      961 gcggtgctag caaagccccg ctggccgaaa ggagcacgaa cagcgccagg agtagcagag
     1021 ccagggcgag caagctccac agcggcaggg tcgggtgtct gcgcccgaag gtcttctcca
     1081 cagatgcctc cttctcgggg tcctcgcccc ggtttgggat cacgggcgaa ggccagtctc
     1141 ctgactcccg gctcttcgct aacccggcct tcccgctgct gcggtggccg cttcgggttc
     1201 gctccccggt tacagtggcg ggaccgtgcc ggactcgcac cggcttcctg ggccccttcg
     1261 ccccctgtta tgtcctcgag cagcatagcc gggcttccac cacgcgtcaa ccggcgccgt
     1321 gcatgaggcc tcatgcacga cgagccctca gctttcagct tcttgttctt gctgaccgct
     1381 gatagctgcc agcgcgggat ggggaagggt cctgcccttc cccatcccgc gtgatataac
     1441 ccatcgggcg catggaaagc cttctggact tcatcagatc catcccagat tcacccagtg
     1501 ctgtgctggg tttcgtgacg aacctgctcg tgacccacgg ctacctggta atactcttcg
     1561 gggccgcact ggacaatttc ggccttcccg cttcgggaga catcgttctt ttcgcgggag
     1621 gattgttcgc caacggtgga caactcgcgc tcccgctggt gatgctcagc ggcttcgccg
     1681 gtgccttcat ctcggacaac tccgtgtact ggatcgggcg catcggcggt aggcccatga
     1741 tctaccgtat cctgaagatg cgtttcatcc atttcctggt tagcgagagg agcctcgcca
     1801 gggtggaacg ctacttcgag gcgcacgggg ggaagacggt cttcgtcgga cgcttcggac
     1861 caggtctgcg cagcatgacc ccactcttcg ccggggtaac caggatgagg tactaccggt
     1921 ttctccccta caacctggcc gccgcggtcg tgtgggccgt ggcctactcg ctggtcggct
     1981 acgtcttcgg ccagtattgg gaagagctac tcgcgaccgc gaaatccctc ggttacggcc
     2041 tcataggaat cgtcgccttg ttggtcctcg cctacgttct caggcgacgc cggttcagga
     2101 acaacctgga caactaacct cccgtcggct ccgcgccgag cgcatctatc gcccgccaca
     2161 gcgtcccggg gcgtcatgta tacagagcta tgaacttctt cgcgtagtac ctggcccagt
     2221 tggcaccagg ggcgacgagt ggttgctcgt tggcgaccag tacgagcatg tggacgagct
     2281 cgctcttggt gaagctggcg tcgaggagtt ctcccagcct ctcccgtaga tagtctgcgt
     2341 accacaacgg ccattctggg tcggcgccgt ccgtctcgat gtaggcctga tggtgggctg
     2401 cgccgacctc gtgaaagaga ccttcgagct ggccggcaag atccgtctcc atcatctcaa
     2461 cctccccagg actacgtgat ctgtgtaaca cacgtcatta tgttcctctt tcgagaacct
     2521 cacaactgca ctgttgattt ccgcaagaga ccccataacg aaccccacgg cccacagcgg
     2581 tagctggccc ggcagaaatc tgctcgaatt tgctatctct tggaggacac cccggttatg
     2641 gcagcgtcac gaattggccg aggaactccc gagccgaggg cgtgtcgagg cggtaggtga
     2701 cgttcctgga tcggcacggg acgtccccgg ttatcgtgat ggacgcgatg acgcgtgagt
     2761 cgccaaggaa gttcggcccc ccgagtcgcc gtagcaggtg ccggcgtcgc cggtggacgg
     2821 gttctgcgag aggcgcagga agttcttgtt gagcgcgcta aaggtcgaga ctgcgtacat
     2881 cctcgtaccg tcctcgccga agaccggggc gccgctgccg ggctcgtggg tgcgttctgt
     2941 ggccccgtag cctaccgcgg tgaagctttg gcctttgaga gcgccgtcct ccgccagccg
     3001 atcgagcaag cccgcgccgg gcagcaaggc gggctggatg ccgctgacct cctcgtctag
     3061 gacgaccgcg gcgatgtcct tggcatcgga actcggcccg gggaagtcgg ggtgggcgtg
     3121 cggcgttccc ggatacacca cggaagtgtc ggggtcgaag acgctgtcga acgtaacacc
     3181 tcggaactct aagccgaact ggtcgatagc ggccatgcag tgcgcggcgg tcagaaagac
     3241 gtccggggct atcaaggtgc ccgagcagaa caagaactcg ccttgctcat cagctaccac
     3301 catggcgcca acgtaagggt gcctgtcgcc atcgggctgc ccgtaggtga tggcccaagc
     3361 cgccccggcc gccagcaaga tggctaaggc catcgtcgcc agcaagagta tcgctttcct
     3421 catcagttcc ctcatttgag gggcggagct cgtcagcccc gccttgtcgt acaacgctac
     3481 cacagtagca tgtattgggt accaggcgcc cctgccacat ggcgtatttt gtaggttggg
     3541 tagctacttc tggggacgaa ctgcgtagaa aggccctttc tacgcagtta gggtcgtaga
     3601 cccatcgcgg ttccctggcg ccgccgaaca cgacgcaacg gagcattcgg cggtcgaggt
     3661 cgcgtatctc ttcccgccag cggtcgggcc tctcgtgcca taggtggtag gtcccctcgg
     3721 agtcctccta cggcaggtag aagtctcgcg ccacgtccgg cccgtgggcg aggcgctctc
     3781 cctccgtggg caggatcccc aggcctgacc cgtccggcgg aagagcccag actctatggg
     3841 aggtctgggg cgtagaaggt aagggtgaca gaaaaccgct cggggtctgt ggctggctaa
     3901 ctaccggagt tgtgggagct gaactactgg ggacagcagt tgtagctctt ggaatacagt
     3961 ttggagccgc accgccacat acaagagtcg gatccactcc tgtatgagct atggaggatt
     4021 gaccgccgaa ggacgaagag aaatcgccat acttatgtca tttttcccgc ctttgagatt
     4081 gggatttatc gttgctgatc gtaatccggt agctccggct tctgcaattc gctcatatgc
     4141 taccgcgtga gcatcatcat tcccgatgac aaaattactg acgcgggtca tgcctgcagg
     4201 agccttgatg tcgggctgtg gatcatcatg cgcaaaaccg cagaccagca ttcccccgtt
     4261 tggcgcattc acacttgggc agttaatttt agccggatcc gctgtttcag gattgacagc
     4321 ctttgcaaat acggggcttg cttgatctac tcccctgaaa gtcaggatgg tgcccccggc
     4381 aagcggatca tgatttagtg tagtattcac tacgttccac gtatatgacc gtggctctgt
     4441 agctgcggca atcctataat atgcgtgaag tgatagatcc ctaccctcca aatgctcatc
     4501 aatcaatttc cagcctgaag gaggagttcc acccacatta ccataatccg cctgaagtag
     4561 agcaagcata aaatcaccct gctctgtgcc tggaggtacc ggaacggtaa ccttaccttg
     4621 tggagttgtg tctgtatcac ctgttgctga gccgatatag gtcaagctac cgccaccacc
     4681 gctggggggg aaggtcacgt aatccaagaa cggcttgcgc cccgagcccg tgttggcagc
     4741 ctttacgctt atctgcacgt cgttccccga aggagcgttc acgtcgaagg tgtaggctcg
     4801 gggagcgccg ctgttggtta tggcctgtgc cggtgcggta aaggtgccgt tgacgctcac
     4861 cctgagcttg ggcgagccac cactctgtgt ggcacgggcc atcagcacca cgtcacccga
     4921 agagttgaag gtcagcggct ccttagcgat tgcggtgtta ttggagaacc tgagcgcctt
     4981 gccgttagag tacatagtgc cggttacgac gctggtgccg gtgggcttga cgtcgaagtt
     5041 ctcgccctcg acggctacat cagcagcatg aactaccttg agtggaccga acacacctag
     5101 caccaccacc gccgccaaca ctgcgagcaa gcagaagatc ggatggctca gtagcagccg
     5161 tgaggccgcc cgcattgtct ctgactttcg catcccccac acctttccca gccgtgggtg
     5221 gactcgccct ttacccactg ctcttgcatt aaccgcgggt agtatagcag tggtacaagg
     5281 ggctatttat atgccaggta gcaatttctc tggtagggac ctcctttgaa atgccgcggc
     5341 tgggaaagcc ggaacggcca gcatgatccg agtcccacgc tgataaactc ctctattggt
     5401 gaagaaactg gagagacgag cttgtctggg ctgaacaccc gggaaaggga ggatctggag
     5461 cgccgcaacc acgagctctc ggtgctcaac gagatcgcgc gcgaactcaa ccgctccgtc
     5521 gagcttggcg aggccctcgg gttcactttg tcgcaggtgg ccgagctgct gggattgcgc
     5581 acggcctgga tctggctcat agacgattcc tctcccgagc cctacctggc cgcggcccgc
     5641 aacctccctc ctgccctcgc ggacgacccg cgccgcatgg acggatcggg ctactgttat
     5701 tgcctggaca cctacaagaa gggtgacctg gaaggagccg ccaacgtcaa cgtcctgact
     5761 tgcagccgcc tggacggcct cgtagacggc actggtggcc tgcgctacca cgccagcatc
     5821 cccctctacg ccggggagaa gaagctcggc gtaatgaacg tcgcgagccc ccagtggcgg
     5881 ggcctctcgc cggaagacct gcagctcctc tccacaatag gagacttatt ggctatcgcc
     5941 gtcgagcggg ccagcctctt cgaccgcagc gccaggctcg gcgccgtcga ggagcgcaat
     6001 cgcctggcgc gagagatcca cgacacactc gcccagggac ttacagccac gggactccag
     6061 ctcgagtcgg ccgaagcctt actggacgat gagttcggag cgcagcgcgc ccgcgaaccg
     6121 ctgcgccgcg ccctctcact cacccgttcc aacctcgatg aggcgcgccg ttcggtcctc
     6181 gacctccgcg ccgccccact cgaaggccgc tccctccccg aggcgctaaa gaccctcgtc
     6241 gaccgctggg aagcagagac tgatgtggcc acgagattca gggcggtgaa cggcagtcgc
     6301 cctctcccgc cgcgggtcga ggtagccctc taccgagtct gccaggaagc cctcaccaac
     6361 atcgccagtc acgcgcgcgc aagccgcgta acggtcaggc tcgtggccac accgcaggcg
     6421 gtgcgactca ccgtcaagga cgacggacac ggtttcgacc cttccagcgt cgccgatgcc
     6481 cgccatggca tcgtcggtat gagggagagg gtcgagatac tcggcggctc tctggtgata
     6541 gagagtgtcc ctggcgaagg tacgcgcctc gacgttaccg ttccgctcga aggaatacgg
     6601 ggtggataag gtaaggctcc tcgtcgccga cgatcacccg atgctgcgcg agggtctcgt
     6661 agcggtgctg gccacccagc ccgacttcga ggtggtgggc gaagccgcag acggggatga
     6721 agtcgtgagg ctcgcagaaa gcctggaccc cgatgtgata ctcctggatc tggagatgcc
     6781 tgggaccgat ggcgtcgagg ccctcgaaaa gctccgcgac gccggctcta gcgcacgcac
     6841 tctcgtcttc acggcctacg acacggacgc ccgaatactc ggctctctac gcgcgggcgc
     6901 ccgtggatac ctgctcaagg gcgcttcccg acaggagatc ttcggcgcca tccgtaccgt
     6961 tcaggccgga ggctccctcc tcgaaccggc cgtcacagat aggctcctgg agcgcataga
     7021 gggcgaggag aacgtcccgc agaaccgccg cctgacatcc agggaactcg aggtcctcgc
     7081 gctcctcgcc cgcggcctga agaacgccga gatcgctgaa cgactcttca tctccgaacg
     7141 gacggtcaag ttccacgtgg gctccatcct agccaagctc gaagcccaaa accgcaccga
     7201 ggcggccaga atcgccgtcc gccgcggcct cgtcgaggcc ccctaagccg ctaaaccatt
     7261 tcgcctcaac gcccctgatg cttgaagcct tgctgtacca aagtacaggc cgaaaaccgt
     7321 ccctcctgac gtttcccggc gccggtcaca gcgatacatt gtattcaaaa caagaactgg
     7381 aggcatacga aatgaccgga gaatcttcgg atcgcacggc gatcatcaca ggtgcttccc
     7441 gcggcctcgg tctggcgctc gcccgcaccc tggccgggga gggctggacc ttgatcatcg
     7501 acgcccgagg caaagaagcg ctggagagcg cccgcgccga gctatccaga gacgcgcgcg
     7561 taatcgccat accgggggac gttgccgacc cggaacatcg agaggatctc gccgaggcgg
     7621 cacgggaggc cggcggcgtt gacgcgctgg tcaacaatgc gagcgtcctc ggcccgagcc
     7681 cccagccgga gttgctcgac tacccgctcg acgtgttcga gcaggtctac cgaaacaatg
     7741 tggtcgcacc gctcgcgctt attcagacgc tccacggcga gctgaaacca caggcgcgca
     7801 ttatcaacgt cacgagcgac gccgccgtcg aaccgtacga aggctggggc ggatacgggt
     7861 cgagcaaggc cgcactggag caactctcca acatcctggc ggccgagaat cccggactgc
     7921 gcgtctaccg cgtggatccc ggcgacatgc gaacccagat gcaccaggaa gccttccccg
     7981 acgaggacat cagcgacagg ccgctcccgg aagagagcgt tcctggactc ctcgaactct
     8041 tgacgggcga cctgccgagc gggcgctacg aggccagcgt actggccgcg agggtgggaa
     8101 cgaccaccgc ggcctcatag aaggagagga ccagattgtc acacacgctc acagaagctt
     8161 tggaaagcct agacacattt cccgactatc ccgaggcgag atccccgctc cgcttcgagt
     8221 tgcctcggga gttggaggcc ggcgagccgc ccgaggcgcg aggactgcgc cgcgacgaag
     8281 tccgcctgat ggtctcctac ctcgatgacg accgcatcgt ccactccagg ttcggcgatc
     8341 tcccggagtt cctgcgtgcc ggcgataccc tcgtcgtcaa caccagcggc accatgaacg
     8401 ccgcccttcc cgccgagcgc agtgacggca cacccctcac cgtgcacctc tcgacccacc
     8461 tgccggccga cctgtgggtc gtcgagctga gatcctcctc cggccatgaa cctgtgctcg
     8521 acggtaagtc tggtgaggtc ctttcactcc cggcgggtgc cagcatggtc ctgcacaccc
     8581 cttatttgtc ggagaggcgg cagcgcgacg atgagtccaa ccggctctgg atctccaccc
     8641 tgaagctgcc cgtgaatctc aatgattatc tggacctcaa cggatcaccc atcaggtacg
     8701 gctacgtcag ggagagctgg cctgcgagct attaccagac ggtctacgcc accgaggtag
     8761 gtagcgccga gatgccctcg gcgggaaggg cttttacgcc ggagctgatc acccgcctgg
     8821 tcgcaaacgg ggtgagcgtc gtgccgctga tattgcacac gggcgtagca agcctggaag
     8881 acgacgagcc gccatacgag gagttctacc gtgtccctcc cgagaccgct gccgcgatca
     8941 actcggcccg cgcggccgga cgcagggtgg tcgcggtagg gaccacggtc gtgcgcgccc
     9001 tcgagaccgt caccgacagg gacggctcga tccacccggg cgagggctgg accgacgtct
     9061 tcataacccc ggagcgcggt atacgttccg tcgacgcgat gctgacaggt ctacacgaac
     9121 cgcgctccac ccacctgtct atgctggagg cgctcgtcgg aagcgtgtcg gaacacccaa
     9181 tctccacctc cggcgcggag catctcgaag tcgcctactc cgaggccttg aagagaggct
     9241 acctctggca cgagttcggg gacttgcatt tgatcctgcc tggtcactag aaggtttcag
     9301 gtatcaggct acaggcttca ggtggtgttg gacgggctcc aaggcaactg cggagccccg
     9361 caccacttcc aaggccaagc accccctcat gaagtctcct cactgaaacc ctacctgagg
     9421 cctcatgtac atgcgacgac ggctttgccc ggcttccaac ctggcgggtc ggtacacagc
     9481 cagccggcgg cctcgcgggc cgtgcgctca tcatcggcct tcatatccat gcgccaggcg
     9541 aagatgcggg acaggctcgc ctgatccctg ctgttgaaag cattgacgaa ggagatcaca
     9601 agccctgcca cctgccgtgg gcggcaaccg tcagggagcg agtcgtcgtc actggtgacg
     9661 atcacccgcg taggttcctg gtgaattaca gaaacaggct gtctattcgc gtccccagac
     9721 cgcgacttcc cggtctcccc agcttcctcg cacccaacaa agaggatgaa gggtatcaga
     9781 atggatagaa tcgccgcttt caagggagaa tccgagatcg ccccgccttc caccaccccg
     9841 catggccgac tccattctaa aacgggggta gcagagaggc gttgccgctg gatcgttcac
     9901 ggcgcgccac acaggcaacg acctaccgcg aatcagaaag aatccggtgg ttgcttccgg
     9961 tcggaagggc ggagcttctt gcgcgtcgtg gattcccgct ttcgcgggaa tgacgggtgg
    10021 gaccgtgaga atcctgatac ctgatgcctg taacctgaag cctaaaacgt tgaaatgcta
    10081 cctcaaagag atataagccc gtgccgcgcg ccctggctga ccgcctcggc gcggttgctg
    10141 acgccgagct tggagtagac cgaggagatg tggaacttga cggtatgttc gctgatgtgg
    10201 agctcgcgcg cgatcatctt gtttgacagc ccatgcccga gcaggtcgag cacctcgctc
    10261 tcacgcgcgg tcagcggctc ggagagcttt tccaccgccg ccgcaggctc gtcgagcaac
    10321 cgctcggcca ggttcctcgg gagcacggtc aggccctggg ccacggctgc gacggcggca
    10381 cccagctcct cggatggggc atcgggcggc acgacgcccc atcccctcag agacagggtg
    10441 cgcagcctgg acgccgtgtc ctcatcctcc gagagcagca gtagcgacag cgtcccgtct
    10501 tccgagacgg agagtgcggt ctcctcgagc aactcctcgt cggcgacgag cactacatcg
    10561 gctccagaga gcggggggac gggtcccacg gacagaccgg tctcgccgac taccagcaag
    10621 tcggcggcct cgacggacgc gagcatcgaa cgcagccccg cccgcatggt cggcgtcggg
    10681 gccacgacga atacccgcgt gggcgtcatg cggcgcgtcc cgactcttcc aggcgcactt
    10741 ccatgaccat gattttaccg ctgcgcatga cccgcaagga gacgacatca ccggcgtgcg
    10801 ccacggcgtc caacagcgtc tgtgtctccc cgagcggttt cccggccgca gcgagcaaga
    10861 cgtcgccgac cagcaggccg gccctgtagg cggcgcctcc ggtctcgacg ccggcgatca
    10921 cgagccccgc agtgccggct tcccgctgct gggatactgg cacctcgacg ggcaagaccc
    10981 cgacacccag cctcggacgg cgttcttcgg cgatccaggc ctccgccgcg ttgcttggaa
    11041 tggagagcgc catacggccg aatatcatgg cgttgatggc gacgacctca cccctggcgt
    11101 tcaggagcgg tccgcccgag tttccgggcg cgagggcgac gtcggatctt acgtagcgcg
    11161 cggaggaact ccgtccctgt ccgcgaagct cgcccacacc gccgacgatg ccggcgctca
    11221 ccgcccccac actgccccaa ggatgcccga ttgcgtagac cagttcgccg acgcgcaggg
    11281 cgtccgagtc cccgacggtt gccgccggca ggtctgcctg gccgccctcc agcccgagca
    11341 gcgcgaggtc caggctccgg tcgctcttta gtacttcagc gtcgatcgtc cggccgtcgt
    11401 ggagtgccac cctgaccgta cgtctactgc gtcttcccga gaccacgtga tggttcgtca
    11461 ggacgaggcc gtcgccgggc cagatcacac ccgccccgat gccccgcccc ctactgcgca
    11521 cctgtacgac gctcctctgc gcggcctcga tcatccgcac gcccgcaagg gcggcggagt
    11581 ctccggataa ttcagccatc tcgcgatctc ctaaccgcgc tcgcctacgg tgatctcgac
    11641 ctcagcgagc tcgccgccac gcacgagctt gactggtacg gaactaccta ccctgtctcc
    11701 ggccagcagt acgagaaggt cgtcggtgtc ctcgacgggc tggccatcaa gcgtcgcaag
    11761 gatgtcgccg actatgaggc cgccacgtcc cgcgggactg ccgtcctcca cgccaaccac
    11821 caggagccca cccctctgtg taaggccaag accctgtccg tcggggaggc gaaccggctg
    11881 gctcaggata ccgaggtatc cccgcttgac ggagccccgc tccgagagcg atcctgccgt
    11941 acgccaggcc aagtctgcag gtatggcgaa agccgcgccg cgtccccagc ctgagaccag
    12001 gatgccgagt acgttccccc gcacgtcgat gagcggtcct cccgagaaac cggggtacgg
    12061 cgtcgcgtcg gtctggatgt aacgttccag cctcggtccc cgccgggtgc ggaccgggcc
    12121 tcccacggcg ctaaccaccc ccagagtcgc cctcggccct tcgccgcgag agcgactgcc
    12181 aacggccagt gagatctggc cgacccgggc ttcgctctct gcgggcgtgg ctggttctac
    12241 atcgaggccc tccacgtcca gcaccgccag gtccgtggag tgatccctcc ccgcgaaccg
    12301 cgccgttagg gtccttccgt cggcggtctc taccgagagg tcttcctcgc gctcgagtac
    12361 gtggctcgcc gtcagcacct tgttctgcgc aaagaccacg ccgctcccag aacgacgcct
    12421 ccgtccgttg accttcacca ccgatgcggc gatgttttcg acagcgtcag ccataccttc
    12481 cgacagcgcg gccagggtat ccgtgtgctc catgatcgtc tcctgatctt gttggtttac
    12541 acggacactc tatccccgga cccgagaccg ggcatcaccc gtacggctag ttttggcccg
    12601 gaagaacggc tagtatcagc atttcagcac gctcaggcct tgcggccctc gctcgtcagc
    12661 atttcagctg gtcagcttct tgtttccact cgccgcaggt agaatgaaga gtctcggtag
    12721 atcgtgtagc gccttcgcgc gcagtcgagc tgtggaaggg agggcatgaa ccggaagagg
    12781 agggccgaag agttgcgaag ccaccgctgg tacggggtgc gcgacctgcg ctcgttcggc
    12841 caccgttcgc ggaccaaaca gatgggctac gctgcggagg actacagcgg gaagccggtc
    12901 gtcgggatca tcaacacctg gagcgacata aacccctgcc acacccactt caaggagagg
    12961 gtcgaggagg tcaaacgtgg cgtctggcag gcgggtgggt ttccggtcga gctgcccgcg
    13021 atgtctctct ccgagccgtt ccagaaaccc tcgacgatgc tctacaggaa cttcctcgcc
    13081 atggagaccg aggagctctt gcgctcccat cccatagacg gcgctgtgtt gatgggcggt
    13141 tgcgacaaga caacaccggc cctgcttatg ggtacgatca ccatggacct gccggccatc
    13201 ttcgtccccg caggaccgat gctgcgcggc aactggcgcg gaaatactct ggggagtggt
    13261 agtgatgtat ggaagtactg ggacgagaaa cgcgcgggca ccatcggaga tcgtgagtgg
    13321 gaagagatcg aagacggcat cgcccgctcg ccgggccact gcatgaccat gggaacggct
    13381 tcgaccatga ccgccgtcgc cgaagtcctc ggcatgaccc tcccgggagc ctcctccatc
    13441 cccgccgccg actcgggaca ccccaggatg gcctccataa gcgggaggag gatcgtcgag
    13501 atggtctggg aagatctcaa gccctcggac atcctggacg accgttccta cgaagacgcc
    13561 gtaacggccg tgatggccct cggcggctct accaacgccg tcatccacct catcgccctg
    13621 gcccgccgca gcggcgtacc cctggacctc gcccgcttcg atgagctctc acgccgcgta
    13681 cccgtgctcg ccaacatacg tcccagcggc gagtacctga tggaagactt ctactacgcg
    13741 ggtggtctac gcgccttgct ctcccgcctc gaagaggtgc tccacctcga ccgcgccacc
    13801 gctggcggtc ggactatcgg tgagaccctc tccagtgtct acaaggatga tgtcatcaga
    13861 ccgttcgacg atcccctgca ggaagagggt gggctcgccg tgttgcgcgg caatctggct
    13921 cccgacggct gcgtcataaa gcactccgcc gcagacccga agcttctcaa gcacacgggt
    13981 ccggccgtgg ccttcgaagg ctacgacgac ctcgccagac gcatagacga cccggacttg
    14041 aaggtggacg aagactccgt tctcgttctc aggggtgccg gccccatcgg tgctcccggc
    14101 atgcctgagt ggggtttgct ccccatcccc aagaagctgc tcgaacgggg agtaagagac
    14161 atggtgcgca tctccgacgc ccgcatgagc ggcaccgcct acggtacctg cgtattgcac
    14221 gtcgcccccg agtcatacgt cggaggcccg ctcgccctga tagaggacgg agacgaagtc
    14281 gagctcgatg tcccgaatcg gcggttggaa ctcagagtgg acgatagaga actatcccgt
    14341 cgccgcgccg cttggagaca gccccaacca cactacggcc gcggctacgg ggccatcttc
    14401 gccaggcacg cgacccaggc caacgaaggc tgcgacttcg acttccttgc ccggggacca
    14461 ctcgtccccg aaccgaagat ccactagccc ccgacgcgtc gcgagggtga tctctctatc
    14521 cagcgattac cagggaacag ggcggcggtc ctggaagaag ccgccggtgg ggccgctgtt
    14581 tggtagcgtc gcggcccaga cgagggcttc cgcgccttcc tcgacggagc gagtggcgcc
    14641 ggagccgccc atatcggtct gtacccaccc cggattgaca gcgttgacga ggatgccgct
    14701 cccacgcagc tcggaggcca ggatgcgcgt cagggcgttg agggcggtct tcgagacccg
    14761 gtagcccggg gagcctccac ccatgtcctc cagggcggcg agccccgtcg agacgttgac
    14821 gatgcgcccg tagccgttgc gtcgcatgag aggaatggcc atcctgcaga ggcgccaggc
    14881 cccgaacagg ttcgcctcca tgctctcctt cacccggtcg aggtcggcgt ccgcgccgct
    14941 ctggccctcg tcgttggaga tacctgcgtt gttaaccagg atgtcgagcc gcccgaactc
    15001 ctcctcgatg gagcgcgaga gccgttctac gctctcgggg tctgcgacgt cgagttgccg
    15061 gacgaccacg ccaccagaga gtcccgccgc ggcatccctg ccttgctcac cgtcgcgcga
    15121 acccatgatc accgttatcc cccgctccgg aagctgcctg cagacctcta gcccgatccc
    15181 ccggtttcct ccgcttacga gcgcgatccg actgttctcc attgtttgtc ctccctatct
    15241 tccgataagc tcgaacgccg cctctacagc cgcgagcggc gacggcgcca aggtcacatg
    15301 ctctccgaga tcccagcctt ccaggaccac cacgggccgc ccgacctttc tggcgagccc
    15361 tatctccgag agcgtaccgt actcgccgcc gacggcgatg acaacgtctc ccgagcacac
    15421 cacagcaagg ttcctcgcct gtcccgtgcc cgtggccaca gagtgcgtaa ggtattcgtt
    15481 ctccctcctc ctgtcctcgt cgggcaggat gcctactgcc gtaccaccgg cctctgtggc
    15541 cccgcgggcc gcagcctcca tcacgcccga gagtccgcca cagacgacgg tacctcccct
    15601 ccggacgatg agcctgccga cctcccgcgc cttctcgtag tgttcgtccg tggctaagcc
    15661 tgatccaaca acggcgacgt acactctgcc ggtcatacgt aatccggtag aaggctcgtg
    15721 ggttgatttg aggagcaccg gccgcagtgg attcccgctt gcgcgggaat tacggtcatg
    15781 ccgggagcaa gggcgtatgg gttgcactca tgttctatag tacggaactg atcgtgccgg
    15841 ttactcgtca cagggtcggg tagcggctcg tctgtccgag tatgtacctg atgagactaa
    15901 cggccccatc cttgctcagg ggctcgttcc agttgccgca cttgggggac tggatgcacg
    15961 cggggcatcc cctctcgcag ggacagcggg tgatgacgcc tagagtgtcg cgggctagcg
    16021 actcgaaggc gtcgaagcct cgcctggaga tgccgactcc tccgggatag ccgtcgtaga
    16081 cgaagaccgt ggggagacgg ctctggcggt ggacgggcgt ggagagtccg ccgatgtcgg
    16141 cgcgatcgca catagcgaag agcgggagga gcccgatcag gccgtgctcc ccggcgtgca
    16201 gtgcgccacc gaagctctcg aagctaggcc tcgcttctct ggggagcgga ggcagcgtga
    16261 cccagagcgc ttgcgtctcc agaactacgt cggggagatc cagggggtag accccgacct
    16321 ccttgtcgtc agcaacccta accttcttga agaaggtaac agaatccgtc gtcctcaccc
    16381 tgccccagtg gagcaccgct ccattcgcga ggtcccgctc ttcgacctcc tccaggatct
    16441 ccacgtcggt ctcgacgcgc ggcctggtgt agtatcgatt ggggacgcgg cgggcgacgg
    16501 cgcggtggga ttggagatcc aggctctcca cctcatacgc ggtcccgcgg tggaggtagg
    16561 tcgcgccagg atgcagctcg ctcggggcgc gggtagcatc ggcggtcccg atcaactctc
    16621 cttcaccgtc ggctattagc accgtctcgg acgatgccga gcgcagtgag atgttacgcg
    16681 cggggttgtc cccgccgacg tagaccagcc ggcttccgct cgaagccagc gccctggcct
    16741 ccatcaactc tttggccacg ctcctgtagg caggaccgaa gtattcctcg tcgtccgcgt
    16801 cgagcggggc ctcgtgggct gcggcgagca ggtgcgggcc gaggatgtag gggttctcca
    16861 tcgtgaccct ggcggcctcg actctcctgc caagaaccct aggcgggttc tcgaagagga
    16921 actggtccag agcgtcgcgg ccggggatgt acacggcgag ggcggggtcc ttgccgcgtc
    16981 cggcgcggcc ccactgctgc cagatggagg cgacgcttcc cgggtatccg cagcagatca
    17041 cggcgtccaa ggcgcctacg tccaccccga gctccagcgc gttcgtggag acgacgccga
    17101 gaaggtcgcc ccgaaagaga cggccttcga tctctctcct ctccctcggg gtgtagcccg
    17161 cacgataagg ggagatcctg cgcgccccgt cgacacccaa cctgtctgcg gcgtaacggt
    17221 agatgagctc ggcggccttc ctcgtcctgg cgaaagctat ggtcctgacc ccttgcgata
    17281 cgagattcgt gaagaccaga gcgccctctg tcagaagact ccgcctctcc cccttctcct
    17341 tgtcgagcaa gggagggttg cggaagatga cccgccgctc gcccgtggaa gcgccgtcgt
    17401 tgtccacgag ggcgaaaggc atgcccgtga ggctctcggc tagttcctgc gggttggcta
    17461 tcgtggcgct cgtgaggacg aagcggggat ctccaccgtg caacatcgcc acccgcctca
    17521 gccgccttag tacggccgcc acgtgcgagc cgaagacgcc gcgcaacacg tgcgcctcgt
    17581 cgacggccac gatctccagg ttgcgcaaaa agtcgcccca ggcctcatga ttgggcagaa
    17641 taccgacgtt cagcatatcg gggttcgtaa gcaggatgtt cgccctgcgc ctgatgtccg
    17701 cgcggagggc gtgcggcgtg tcgccgtcat aggtcgcagg atgcaccccg cgcaaatcga
    17761 aagccttgat cttccccagc tggtcctggg ccagtgcctt cgtcggatag agaaagaccg
    17821 cccggctctt cgcccggcca agcgcattct gaaacgccgg gatcttgtag cacaacgact
    17881 tcccactggc cgtcgccgtc gccaccacca cgttctcacc ggccagcacc cgctcgtaag
    17941 cctccagctg gtgcgagtac aacctcgaag cgcccatctt acgcagcgcc tcggaaagct
    18001 ccggccggag gtcgggaaac tttacaagcg tagcgtcccg cggcgacaaa ctcgccacgg
    18061 tgtcacccag cgactccacc gtccgacggc tcatcccagc cagaggactc tgaacctgca
    18121 tgttagtcat ggagcttctg caaagctatc ggcacaaacc caaaaacctg aagcctgaaa
    18181 ccttggtttg cttcgcaaac cgctacagct cacgtcttcc ggtgaaggcc cgtcccaggg
    18241 tcacctcgtc ggcgtactcc aggtccccgc cgacggggag gccgctcgcg agggccgtca
    18301 ccctgacagg caggtccttg acctcctgcg cgataaacag cgcggtcgcc tcgcccgtag
    18361 tgttcgggtt ggtcgcgatg acgagctcgg tggtgccttc ttcacggacg cgctccagga
    18421 gctcggctat ccggaggtct tcgggctcga cgccgtcgag tggagagagg gcaccgccga
    18481 ggacgtggta gagtcctcgg tactcgttgg tccgttctat aggcccgata tcgtaggggt
    18541 cttccacgac gcagatcacg gacgcgtcgc gacgggcgtc gcggcagata tcacactctt
    18601 cggcctcggt caggttgaag caccgcctgc acggtttgat cttctccttg acctcctgca
    18661 gcgcctcggc gagcccgata gcgtcttctt tcctacccct gatgacgtgg aaggcgatgc
    18721 gctgggcgct cctcggtccg atggagggca gcttggagag ctctgttata agtctctcta
    18781 ccggccttgc gtaggtcgcc aaaaaaccct tacagacccg gtatgttcag gccgaggtcc
    18841 ccgaggccgc cggtgatccc gccaagcttc ttgttggcca gctcctggga gctcgtcatc
    18901 gcctcattaa cggccgcaag taccatgtcc tgcagcatct cgatgtcctc gggatccacg
    18961 acctcgggat ctatctcgat ggagacgagc tccatactgc cggtcatcgt cgctctgacg
    19021 gccccaccgc cagcgctcgc ggtaaccgtc tcgttcgcga gctcctcctg ggcctgctgc
    19081 atctgctgct gcatctgctg aacctgctgc atcatcttgt ttacgtttct gcgattcccc
    19141 aacgaattct ccctggctat gggtctatgg actatatgag tataagccat ctgagcaggc
    19201 gggccaccac ctttggcccc cgcaccttcc gcgcaaagat gcccctgtac gacgtaaacc
    19261 tgcctcgatc tcgctgatgt cgagctttag ctgtggtatg ccacttcttc ggtcgacatt
    19321 cagccccgtg cagactccag tgatacgagt caggaatcga gaagtgctag cttgatctcc
    19381 actgtttcca gaacctcact tcacagatcc taactcttag cgcttctgtt ctcgtcgaag
    19441 agaccgacct cgcgggcgat ctcgaagacc tcgcgctggt cctggatcac atcgtcgcgc
    19501 cccaccgttc cctgcccggg accgctattc tcagcgaccg taccgtccgt tccgttctga
    19561 cttacggtct tcggagggct ctcttcggaa gtcggtgcgg gttgctctat aggggcggcc
    19621 gtcgggggtt gttcttctgc ggcgatcgtg gccgatgctt ctcctcctgc ggccctgatc
    19681 tcgaggtgcg gctcggaacc caggcgctcc ccgagcaccg cgcccagctt cttcaggtgt
    19741 cccgaatccc tggccatccc gacgtggaag ctctgctcct cgggatagac gagccgcagc
    19801 acactaccgt cgaagctctc tacccgcgcc tcgccgtaga cggcagccgt gagcgcctgc
    19861 cggcggcgct tgagctcgcc tatgatcccg tgccaatcgg aggcgaggtc gataccggac
    19921 tcccgcaggt catcgtggtc ttcgagaatc gaagcatcgt cggccggcgc gtccggctct
    19981 gcggtttcct gcgcggactc tacctccgca ggtgactcct cacgtacgat atcagcggcg
    20041 gcccgcgggg ggggcgtggc gacgcttccg ttctctaccg ctctttcgag ggtctccagt
    20101 cggtccagga tggttgtcaa atcgggttcc gtgtaatcgc gggtgagctt gaggaagacg
    20161 agctccagct ccagcttggg atctccgccg cgtcgcataa ggctcaatgc gtcgcccagt
    20221 gcttcgatga gtcgcatgat cccggccgtg gggatggcat tcgcctgctc ttcgagctgg
    20281 gggcgttcct cggccccgac cccggcgagc gcgacctcgg gggcgtaagg gaggagcatc
    20341 aggtttctca ggtgcgcgag gagttcgcca acgaattggc ctagatcctt ccccccgccc
    20401 gagagtcggt ccactatgcg cagggcgtcc gcggccctgc gttcgtgcag ggcgtaggcc
    20461 gtctcgatca acgactcggg gccgacgccg cccagcaact cgcgcaccat ggaggccgtg
    20521 atcctaccct ccgcgaacga cgagagctgg tccagaagcc cctccgcatc cctgaatgac
    20581 cccctcccct cgcgcgcgat gacggtcagg gcctccggct cgacctcgat gttctctgcg
    20641 tctgctatct catcgagctt ccctacgagc gtctcgatgc tgggacgacg gaagtcgaag
    20701 ctctggcagc ggctgatgat cgtcggcaag accttgtgct tctcggtcgt ggcgagcacg
    20761 aagaccacgt gctcgggtgg ctcttcgagc atcttgagga gggcgttgaa agcctcggcc
    20821 gtgagcatgt ggacctcgtc tatgatgtag accttcatcc tgccggccac cggtgcgagg
    20881 ttcaccttgt ccctgagatc ccgtatctcg tctatccccc ggttggaggc cgcgtccatc
    20941 tccagaacgt ccatcgagga gttgcccacg atggcccggc agctctcgca agtcccgtcg
    21001 ggctccggcg tgggcccctt ctcgcagttg agacccatcg cgagcacctt agccgtcgag
    21061 gtcttgcccg tgccgcgcgg cccgctaaac agataagcgt gcgagacgcg gtccgtctct
    21121 atggcacgcc gcaacgtcct tacgacaggc tcctgcccaa cgacgtcccc aaagcgccgc
    21181 ggccgccacc tgcggtagag cgtggtgtgg ttcatgagga gagacgtctg ttggattggc
    21241 tcacgcttct agtataccgg accgttcgac tcgaaccatc ggggttccaa gcacttcagc
    21301 aagtcagcac gctccagcct ccggctgtcg cttgtcagca tttcagcatt cgcctgtgca
    21361 cgcccatgct gacatgctga aagcgaagcc cggcgaagcc gggcgtagcg tgctgacaag
    21421 gcgcgaagcg ccgtgctgac aagcgaagcc cgtagggcgt agcgtgctga aatgctgaaa
    21481 tgccgcgcac cccgccttcg acgtaaccgc ataaggcgaa cttccggccg ccgactccgg
    21541 tctggctacc ccggagcaca tgaggttacc cgcttagtgc tgctaccttc cggtcctgac
    21601 acggttcacg gggccccatt gcccgggacc aggccatcaa cgcctgccgc accggtttca
    21661 accgaacccg gttcgcgcct cgggcgggga tctaggccct gctaggcgat cgcaggtaca
    21721 gggcatcgcc agctccccgc ttagcgcggc ctgccgattc taccagaacg ccacacggtg
    21781 tcatgccagg tccaaaggga tgccaacggc acgacgacac cagccatcgc ccacggttcc
    21841 actgccgcaa gcggtggatt cccgcttgcg cgggaatgat gtgtgggagg gtctggacac
    21901 acccacagcc cgaacgcgga gtcccctcca cacgtacttg gcacaacaag ctgaaatgct
    21961 gactaggcct gcggcctatt ccatgagtac atccagtagc agcgctgcgg accaggagaa
    22021 taggatcgag ccgagccccc tccccgtaag ggggtcgaag tattcgtgga agccctcgtt
    22081 cttgcacagc tcgatgatgg tctttcgcag acgctgggcg tgatcctggt aaccataggc
    22141 ctccaacccg tgcatcaaga accagtctat gttgatccag acaggaccgc gccagtatct
    22201 ctcttcggag aacccgaagc cgtgcacatc gtagctggga attggtgtga tggcctcgtc
    22261 cgccaggccg aatccttcgt tctcgagtgt gtcgattacg cgctttgccc gagctggctc
    22321 cgcgataccg gcgtacagcg ggccagcgag gttcggaccg aagtatgcgg gtacaggcat
    22381 gccacagacg aggtcgtagt ccaggtatgt cccgcgatct tcatcccaca gtctctcctc
    22441 gacggcgctc ttggtcttct tcgccagttc ctcgtggggc gtagggtctt cacccacgat
    22501 acgcgcgatc tccgcgagat ccctaccggc tttggacaag agcgagttga agagcacgtc
    22561 ctgcaccagg aagggacaat cctgccttat cttcgcctcg tcgtaatcgc ggtcggcgaa
    22621 gtacttcacc aggtaggcga aacggtcgta cgaatcgctg gtaggacggt ccgaggcgga
    22681 gacggtgtgt atatcggcgc ggtggtagcg gggtatctga tccgacctga ggtgcatccg
    22741 ctgcaggatc gggtcccaca tcggggagtt gtccatgcca ctctcccagg ggtggcggat
    22801 gtacacgagc ccctcgccat cggggtccct ctcccggtag agataatcgt gccaggcccc
    22861 caatctgggg aagatgtact cgaggaagtc cttagccttc gcctcatcct gggcgtgccg
    22921 gtagatgtat agaacggcca tggcgtgaat gggaggcagg accacgcctg aggtctcgtg
    22981 gtgctcgggt gcgtcagggc tctctttggc gtgccagaag ttcggtccgg gaaaatagtt
    23041 cgtgaagcgg gagttgaaaa cgagttgggg caagagaccg ttcttccact gggcttcgaa
    23101 gaggtgcctg agctctctcg tggcgcggtc ctgatcgtag cggctgtagc cgatggcggc
    23161 gaacgccgag tcccagctcc actggtgcgg gtagagcctg ggacccggca tcgtgtactc
    23221 accggtccag ttgtagtcga gcaccgcctt tgcttgatga acaagcatgt cctcttccgt
    23281 ccgtcgggcc atgctctcgt cctccctcga tccggcgttg gctgtcccct cgcggctggc
    23341 ggtcgcctac cagtataatg ccaccatggc ttccaacgag aatagcgcgg atatcctgat
    23401 cctttcgagc gccataggaa gcggccacat gagggccagc gcggccgtgg tgcgcggcgt
    23461 ggatctcctc gatcccgaga agcgttgctc gattgtggat ttcccccaca aagtctcgcc
    23521 cggcgtcgag cgcctgctca gggacgcgta tctgcaggcc ctcaagctgt ggcccacggc
    23581 gtacgggcgg ctatatgagt cttcgggcac gggccaacag ggaaccctcg tacaacaggt
    23641 gcagacggcc ggactcagga ccctggagcg cctggtgtcg agcaccggcg cgcaggctct
    23701 cgtagccccg catttctacg gcgccggggt tctcggggta tacaagaagc agaaccccca
    23761 gatgttcagc gccgtcgtgc tgacggacta cgtgccgcac cccatcgggg tgcctctgaa
    23821 cctggatctc tacgtcgtcg ccgacgatgc ggcggccgaa gacgtggcaa agctcggtgt
    23881 gcccgaggag cggatccatg cgacgggtat ccccatagat cccatcttcg aggaaccggc
    23941 cgacccgagc ccggcacgca aagaggtcct gaacctggac gatgatgacc tccctgtagt
    24001 actcgttatg ggcggaggcc tcggcggggg cgatctggaa gagtctgtaa tgttgctact
    24061 tcaggccagc gaggccatgc acctcgtcgt gctctgcggc tcgaatgaac gcatccgcgc
    24121 acgactcgag cgtctcgcag atagaagagg atacgaggcc acgttcctcg ctttcacgga
    24181 ccgggtaagg gagctcatgg ccggagggac cgtgctggtc accaaaccgg gcggcatgag
    24241 ctgcaccgag gccctggctt cgaggctgcc ccaggttctc tatcatccca tccccggtca
    24301 ggaggaggac aacgcggcgg cgatggtccg ctacggcgcc ggggtcatgg tccgtgagac
    24361 ctcgcagatc ctgggccaga cgctcaagat cctgaccagc ccggatcaca ggcacaagat
    24421 ggtcgaggcg gcgaagaccg cccacaagcc ccactcggca cgttcggccg cctccctcat
    24481 actggctggc ctaccatgag acacccacgc aaccacgtct ggaaagccgc ggccgctacc
    24541 gccgccggcg gcaccgccct ggccgctctc cgaaccgcga gaggccaccc cgttcgttcg
    24601 gcatcgaccc tggtctcggc cggggcaggg atggcggccg gtggccttat ggcttacgag
    24661 tggctcacgc cccgttcctg gctctacggg ccggtcttct ggcacgctcg tacgggtgag
    24721 cgggtggtcg ccctgacctt cgacgacggt ccctgccatc cctatacggg acaattgctc
    24781 gagatcctcg accgcgaagg catcagggcc accttcttcc aggtaggcaa caacgtcata
    24841 cgcgagccaa acctcgccgc cgaggtcgct tcccggcacg aggtaggcaa ccacacgttc
    24901 acccacccgc acctcacgtg gagccggcca ggcacggtca gggaagagat gctccgtggc
    24961 caggaggcca tccacgaggc cacgggcacc ctgccgcggc tcttccgcgt gccccacggt
    25021 tggtatggcc cgcaggtgat ctcggttgcc ggggaactcg ggatgaggtg cgtgggctgg
    25081 tcggtaatgg cctgggactg ggatcgccct cccaaggatg tgatccaacg ccgcatcttg
    25141 cacggcgttg gcccaggcgg tgtcaccttg ctccacgacg gccaggacac ggacgctttc
    25201 ccaaaggccg accgttccca caccgtggcg gccgtctccg acatcatcag cagcctgaaa
    25261 gcgaacgggt acggctttct caccctcccc gaacttatgg acctcgacgc gcgcgagcat
    25321 ccgcctggct gacgacgaac tcgtactcgt tggagagtgg tccttcgtcc ccgagtaacc
    25381 tgagcctgag cacggctggc cccggagcgg ggagcgtagc caccgcctcc ccgattctca
    25441 tcacggagtc tttcggcacc ctagtctcgc ctgtgccgcg ggccacgctc gatcccccga
    25501 ggagcaactc ccagtcccag ccaacttcat ccagctcccg cagaagatcg ttgatgataa
    25561 agagtggcag ggagattctt gtgcccacct cgtagcgctc cttcgggtac tcggcacaga
    25621 acaagaccgg gctcatcgcc gtaaagaggg tctcgtaagc caatttcggc tttcgccgcc
    25681 agtccacgac ggaccaggac accgcgggtg caggatcgtt tagcataaag gcgaaggtcc
    25741 cgcccgtcgg cccgtacttg cgccgccgga agagctccgt ggcgtgtttc agaacctcgg
    25801 cctgatagcg ctggctctcg cgcaagaaaa gcgtccgatc tcccctccac ggaacgtagc
    25861 gggccatacg ctggatctgg aggcgatagt tcagggaaag gacgctccag tcgggcggcg
    25921 tccctgaagg ccagatctcc tcgagcgact cggggtcagg cagcgcctgt gccccatact
    25981 ctgtcggcaa ccgcgagaat accgggaaga ccttcacgaa gcgttccagg ttcctgaact
    26041 tcccgtagta ccagccgaag tagaggtggt tcgtcgtcag gggatggccc gcggcatcta
    26101 tcacgggcct ggagtcgtcc tcctccgaga tcgcacgcac cacggcaggg tcgaggaccc
    26161 gccgctgcca gagtatccat cgtggggaga gaacctcacc ggcggtccgc acgagcccga
    26221 gggggctcca tttctcgggg ggcgccacga agaagggttc gtcgtgcgcc agatagatgc
    26281 ccacggaagg atagccgcgc aggattcgcg ccatctcccc ggcctgggtg acggctggct
    26341 cgaggatgct ccgcctgtgg gtccactgca gaggaaagtc ctggagcacg agaagcccga
    26401 gctcgtcgca ggcctcgtag aattctccgt ccgagacgtg ggcgtggacc ctgacggcgt
    26461 tcataccggc ctcgcgcacc agggaagcgt ccttgcgcag aagctccttg gttgccctgg
    26521 ccgggtaagc gtccgagggc aggtagttga tcccacgcag gaagatacgc tctccgttga
    26581 ggtagacctt ccagtcctcg agctccacgg tactggcgcc gaaacgcgtt tgctcttctg
    26641 ccccgtcaca cgacaacacc agctcgtaca gcgcctgctc gcccctgtcc cacgccgacc
    26701 acacccttac gtcgggcacg cggaagccga tctccaagca gttctccccc tgctcgatct
    26761 ggagctctct ctcgaactcg acgccctgag cctcgaagcc cagcggacga acccgtccag
    26821 ccagctcgac gcgcgcctta tacctggagt agaggtctac atgcgccacg ccacgcccgg
    26881 tgtggtctgc cgtggccgcg agggagcgga tgcgcacccc tccgtcggcc acgaggctga
    26941 cctctttgaa gataccgccc ggatttatgt ggggggccat accgtcccac cttgcccaca
    27001 caccgccgat ggtcttgcgg tcgttctcat ccgattcctc ggggctgaat acctctacga
    27061 gcagctcgtt ctcgccgttg gttatgacca tatcggtgac gtcgaactcg aacgcggaga
    27121 aatatccttc gtggtcgcca aggtagacgc cgttgagcca gacccgcgcg gcgtagtaaa
    27181 cgcccccgaa cctgagggaa agcatcgcgc cttcggaaag tcccccctgc gcaaaccggc
    27241 accggtacag cacgagaccc tcataagccg cgaaggcctc ttcgagctgc cagtgccagg
    27301 gaacgcggac cggggtccag gcacgtttta ttcttgccgc ttcgccgacg ctaccgagcg
    27361 gcgccgcgag cggtacggct tcccaatcat ccagggtttg cgaacccttc ggtcgcatta
    27421 gaatagcgta ccatcaagca tttcagcacg ctgcctctgg cagcttgtcg gcacgcccag
    27481 gcttcgcttt cagcacggcg gctgcgccgc cttgtcaaga atttattgtt gaggactgat
    27541 gggtaactct ggacgtgagc ggtagaagcg ggccgtgcgc tactccctct cgatgcggtc
    27601 taaggtctcc ccttttgcag ccgattggtc tggtgttggc aggccaactt ccggtggaag
    27661 ttcgagcctg agatcccctt cgtggtctat ggaaagctcg ccaccaacca gcttgcatcc
    27721 gagcacgtga ccaccggcgc tcctgtccgc ggtgatgaag tggaagtggt agccagagac
    27781 attcaggcct tgtgcgtaat cgggaaagcg gaagccgacg aggctaccgg agacatcgtg
    27841 aagctcgaag gtgggctggt ccttgacgac ctctacgagg ggtggatagg gcttgcgttg
    27901 gcggggcacg ctacgcgtct tgacgtactc gaaacgtccg tctacccgga tagcggcgca
    27961 cacggttccg ccaccgatga ccccgtctac gtagtcacag agatcctcga agtccatcgt
    28021 catgggaaga gttcggtacg tatccggctc gaatagggtc accacggcga agggggtcct
    28081 ggcatcttcg tcgacagcgt aggcccgtcc gtcggccttt acctggaaga agtcgccgtc
    28141 gaggcagatc atctctccgt cgagggcgtc gaaagtgcct agtccgaagt ctccacgatc
    28201 ccccagttcg gcgaagctga cgtccccatc gtactttccc tctagtaggg catcgacggt
    28261 ggaagtctgg aacagtgtgt ggtggagatg ctcgttcccc ctgagatccc gctctctcag
    28321 atactcgacg tgcagcgcac ccaccaacga ggtatcgata ggcatcaggc cgatctcgct
    28381 ttcgtgaccg ttgattttgc agatcgaatc attagcctcc ttccagtagg caaagcctct
    28441 accgccagcc ggtcctgcgt gccagccagc ccccggccac ctcgccctgc gacgcctgga
    28501 atggacgcag tgtgggcaac cccggcggcg ccggctgtaa cccctcactg gtaaagaacc
    28561 cggcaacggc ggccaccacg gccgtcaggg catcggggtc ggcccggcgg ctgtgcggat
    28621 gatgggcgag gagctcttcg ggtggcgggc cgccctgcat ggccacgctc ggggcgaaga
    28681 agaccgcgtc gacccacgag gccccgacac gggcgtgcgg ccagtccact accagcaccc
    28741 ggtcatccgg cgtcagcaac aagttgtccg cccgcaggtc cagatggagc agggtgcccc
    28801 ctttcgccgc cgcaggtgct tgggcctcca gagcggcgag ctcgtc
//
LOCUS       DATMVF010000158         8127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_613307, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000158 DATMVF010000000
VERSION     DATMVF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613307"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VN178_07960"
     CDS             complement(<1..246)
                     /locus_tag="VN178_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80945.1"
                     /translation="MMGLRNMYKVFRETRSAAREQATLAVTGDSPRAAEVAALLGAQR
                     GVSGAEVILSVSETGVAISGKAVEDPGEITLSSRGGKA"
     gene            380..1474
                     /locus_tag="VN178_07965"
     CDS             380..1474
                     /locus_tag="VN178_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS80946.1"
                     /translation="MQPQRDLRDIEEIQELFEAGQETGTLESSEVLDLLQEVDLSTDE
                     IQQVYGLLREHGVEVVDAGFLAETMGEDEDTQLVKEVLEGDLKSRYTGDAVQMYLDEI
                     GKTPLLTKAQETYLARRIERGDRRAKAHLTKANLRLVVSIAKKYAGRGVSLLDLIQEG
                     NIGLMRAVEKFDYRKGFKFSTYATWWIRQAVTRAIADKGRTIRVPVHMVEKINKYYRV
                     QRGLGAQLNRDPTDEEVASIMEVDAEEIVRIRRVSRRSISLETPVGEDHSSELGDFIA
                     DEESESPHDLAKSSLLKARMREALGSLPERERMVLEYRFGLTGGQPKTLEEVGERFDV
                     TRERIRQIQLTALAKIKSSPHAAGLRDLLE"
     gene            complement(1643..1798)
                     /locus_tag="VN178_07970"
     CDS             complement(1643..1798)
                     /locus_tag="VN178_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80947.1"
                     /translation="MSIAIASLLVLVIVVIIAVPILIRVFGEAARRNPNRPEGEEDET
                     DETEDRE"
     gene            complement(1944..3188)
                     /locus_tag="VN178_07975"
     CDS             complement(1944..3188)
                     /locus_tag="VN178_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWS80948.1"
                     /translation="MWKETKTRSETRISVARVLFLLVVVFATAFGAYMVGRSQSPATL
                     NEEDRESVALYAEALDTVRKNYVDQKSIDSKKETYGAIEGMLDTLGDDGHTRFLTPEE
                     REQNDQSLSGTYVGIGVQLEEKNGEVVVAAPIDGSPAEEAGISSDDVLLAVDGKSVRG
                     DEVSEVVEKVKGPEGTGVELTVRHEGERRTYDLQRAEIDSPVASWTLIPDTDVALVLL
                     SSFSDDSARELQNAFEEAQAAGARRFILDLRNNPGGRLDQAVEMAGFFLEPESVVYIR
                     KDASGERKEITVEGDPESTQAPLAVLVDGGSASSAEILAGALRDNDRAPVVGETTFGT
                     GTVLSEFVLRDGSSILLGVAEWLTPDGDFIRNTGIAPDVKVPLSEGTDPLTPDDARDL
                     SREEILEKDAQLRTAYEKLQSK"
     gene            complement(3408..4484)
                     /locus_tag="VN178_07980"
     CDS             complement(3408..4484)
                     /locus_tag="VN178_07980"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWS80949.1"
                     /translation="MTEGTNGSPKTNGHSGEYLLETRNLKMHFPIKAGVLQRTVGHVK
                     AVDGVDLSVRRGETLGLVGESGCGKSTLARLVLRLLEPTEGEVIFDGEDILGYDRKRM
                     LGVRRNMQIIFQDPYASLNPRMTVGNIVSEPLRTHDVGGSSGERKRRVQELLEVVGLN
                     PEHYNRYPHEFSGGQRQRIGVARAIALNPRLIICDEPVSALDVSIQAQVINLLQDLQK
                     EFDLTYVFIAHDLSVVKHISDRVAVMYLGKIVELADRAALYGEPRHPYTASLLSAIPV
                     PDPQKERERQRVVLSGDVPSPANPPEGCSFHTRCPRAQDYCTNHEPGLEEQEKEGHEA
                     ACFFPVLEGQRIEEPATNTPYSAE"
     gene            complement(4481..5494)
                     /locus_tag="VN178_07985"
     CDS             complement(4481..5494)
                     /locus_tag="VN178_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS80950.1"
                     /translation="MLEVNNLKTHFRTQDGVVKAVDGVSFSLEAGETMGIVGESGSGK
                     SVTALSIMQLNPKPPAEYPEGEILFEDQNLLQASDKRMQEIRGNDIAMIFQDPMTSLN
                     PVFTVGNQIREAIRIHQNLSKSEAQEKTVQVLRDVGIANPERRAGEYPHQFSGGMRQR
                     AMIAMGLSCNPKVLIADEPTTALDVTIQAQILELMVELQEKYGTAIIMITHDLGVVSQ
                     LADKVMVMYAGRAVEQATTDEVFYDPLMPYSWSLLRSLPRLDTAGEVRLLPIEGTPPS
                     LIFLPKGCNFSPRCPFVKDECHEIDPGLEEKKPAHTAACILSVEEIEENKSRVDEELG
                     VET"
     gene            complement(5591..6451)
                     /locus_tag="VN178_07990"
     CDS             complement(5591..6451)
                     /locus_tag="VN178_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HWS80951.1"
                     /translation="MTKIVATFSIVAFDSETNSLGVAVQSKFLAVGSVVPWARAGVGA
                     VATQAMANYNYGPHGLELMAREKTAYETVEELISADEDREHRQVGVVDARGRAATFTG
                     SECFEWAGGITGEHYAAQGNILVGGETVEAMARAYEESGGDLAARLLSALEAGQAAGG
                     DSRGKQSAALLIVREDGGYGGDNDRVVDLRVDDHLDPIQELIRIRDLHTLYFGETMPE
                     DVVAVDGEVRGEVEDALRRSGYIRVQDLGDETLFDALSAYIRTENFEEREQRRGYLDR
                     AVLEYMKGQS"
     gene            complement(6470..7366)
                     /locus_tag="VN178_07995"
     CDS             complement(6470..7366)
                     /locus_tag="VN178_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80952.1"
                     /translation="MSEAVTAEGAQTQAVEPRERRLAAFWRRFRRNRLAVFGLLIVAL
                     LLFTAIFAPLIAPSDPSAQDYALTLRPPGEGGLMGTDSLGSDVFSRVVYGARISLESS
                     LISVGIALLVGVPVGLTSGYYRGFWDEWIVMRVVDSLQAFPFLILALVIAAILGGGLG
                     YAMIAIGIGFIPAFVRITRAQVLTVRGLDYVDAARATGTGNTMIMLRHVLPNSLPPLL
                     VQTTLAMGYAIIAEATLSYLGLGAEPGEPSWGSMLFQAQSYLSTAWWMAFFPGMAIFF
                     AVLGFNLLGDGVREALDPRTYD"
     gene            complement(7369..>8127)
                     /locus_tag="VN178_08000"
     CDS             complement(7369..>8127)
                     /locus_tag="VN178_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS80953.1"
                     /translation="FDWLRGVLKGDLGEFLIDDTPVSQLILERLPVTIELAVGTFAVS
                     LTIAVVAGILSASRRGTWVDYLSTGVALGGISIPHFWLGMMFIIIFAVSLGWLPASGY
                     EPFFQNPVANITAMILPILATGLRESAELTRMLRSSLLEELGSDYVRTAFSKGLSKRI
                     VVIRHAVRNALIPFVTASGLQIAGLLGGLVVTETVFQLPGLGRLVVDSIEQRDFTTVQ
                     GAVLTITIIVVLVNVLVDVLYTIIDPRISVSSGE"
BASE COUNT         1468 a         2630 c         2497 g         1532 t
ORIGIN      
        1 ggccttcccg cctctcgaag agagtgtaat ctcgccaggg tcctcgacgg ccttccccga
       61 aatagcaacc cccgtctccg agaccgagag gatcacctct gccccgctca cgcccctctg
      121 cgccccgagc agggcggcga cctcggcggc cctgggcgaa tcaccggtga cggcgagcgt
      181 agcctgctcc ctcgccgcgc tgcgagtctc gcggaatacc ttgtacatgt ttctcaggcc
      241 catcacggct cgtcctcctt gtagtttggc ccgtacgact cagcccgcgc gggcggaacc
      301 aaattgatta ttgtctcttt taagatatat tacgtggagg ctacaggagg gtttcaacct
      361 gaagttcccg gagtagactt tgcagcctca acgtgacctg cgggacatag aggagataca
      421 ggagctcttc gaggccggtc aggagaccgg cacgctggag tcgagtgaag tgctcgacct
      481 cctacaagag gtcgacctct ccaccgacga gatacaacag gtctacgggc ttctccgcga
      541 gcacggcgtc gaggtggtcg acgccggatt cctcgccgag acgatgggcg aagatgagga
      601 cacccaactc gtcaaggagg tgctcgaggg cgacttaaag agccgctaca cgggggatgc
      661 ggtccagatg tacctggacg agatcggcaa gacccccctc ctcaccaaag cccaggagac
      721 ctacctcgcg cggcgcatag agcgcggcga caggcgcgcc aaggcccacc tcaccaaagc
      781 gaacctcagg ctcgtcgtct ccatagccaa gaagtacgcc gggaggggcg tctcgctgct
      841 cgatctgatc caggagggca acatcgggct gatgcgggcg gtcgagaagt tcgactaccg
      901 caagggcttc aagttctcga cgtatgcgac ttggtggatc cgccaggccg tcaccagggc
      961 catagccgac aaaggacgta cgatccgggt cccggtccac atggtcgaga agatcaacaa
     1021 gtactacagg gtccagcgcg gcctcggcgc ccaactaaac cgcgacccca ccgacgaaga
     1081 ggtcgcgagc atcatggagg tggacgccga ggagatcgtg cgcatcaggc gcgtctcgag
     1141 gcgttcgatc tccctggaga ctcccgtcgg ggaggaccac tcctcggagc ttggcgactt
     1201 tatagcggac gaggaatccg aatctcccca cgacctcgcc aagtcctccc tcctcaaggc
     1261 ccgcatgcgc gaggcgctcg gcagcctccc cgaacgcgag cggatggtcc tcgagtaccg
     1321 attcggcctt accggcggcc agcccaagac cctcgaagaa gtcggggagc gtttcgacgt
     1381 cacccgtgag cgtatccgtc agatacagct cacggccctg gccaagataa agagcagccc
     1441 ccacgccgcc ggactgcgcg atcttcttga ataggtttca ggcttcaggc atcaggcttc
     1501 aggtaaaagg gtggtgggcg tgcctgatga gtgccttccg aactctatga gcgcccttac
     1561 ttggagacta ccggtatcgg agatatagcc tccggccgta ctgcggagag ctgaaaccta
     1621 ttacctgtag cctgatgcct tcttactcgc gatcttccgt ttcgtcggtc tcgtcttcct
     1681 cgccttcggg gcggttcggg ttgcggcggg cggcctcacc gaagacacgg atcagtatgg
     1741 gcacggcgat gatcacgacg atcacgagaa ccagcagaga ggctatggcg atggacacga
     1801 gattctcctc ccctcaagga tatggaagcg cccggggcta ctactactgg cggctcctat
     1861 cctacaagag ggctcactgt agctacaaat cttatcttgc ccgtgaggat gccccttcca
     1921 aacccgtctt tgagtaaacc tcgctactta ctctgtagct tttcgtaggc cgtgcgcagt
     1981 tgggcgtcct tctcgaggat ctcctccctg gagaggtcgc gggcgtcgtc gggagtaagc
     2041 ggatcggtcc cctcggagag gggcaccttt acgtctggtg cgatccctgt gttgcggata
     2101 aagtcaccgt ccggggtaag ccactcggcg acaccgagca ggattgacga gccgtccctc
     2161 aggacgaact ccgagagcac cgtacccgtg ccgaacgtcg tctccccgac gacgggcgca
     2221 cggtcgttgt cccgtaaggc cccggccagt atctcggcgc tcgaggcaga accaccgtca
     2281 acgaggacag cgagcggcgc ttgtgtggat tccgggtcgc cttcgaccgt gatctccttg
     2341 cgctcgccgg aagcgtcctt gcggatgtag acgacgctct ccggctcgag gaaaaaacct
     2401 gccatctcca cagcctggtc gagcctgccg ccgggattgt tcctgagatc gaggataaag
     2461 cgcctggccc ccgccgcctg cgcctcctcg aaagcgttct gcagttccct ggcgctgtcg
     2521 tcagagaacg acgagaggag cacgagggcg acgtctgtgt cggggatcag ggtccacgaa
     2581 gctacgggcg agtctatctc tgctcgctgg aggtcgtacg tgcgcctctc gccttcgtgg
     2641 cggacggtca actcgacgcc ggttccctcc ggccccttga ccttctccac gacctccgag
     2701 acttcgtcac ccctgacgct cttcccgtct acggccagga gcacatcgtc ggaactgata
     2761 ccggcctcct cggccggaga gccgtctatc ggggcggcga ccacgacctc accgttcttc
     2821 tcctcgagtt ggaccccgat ccccacgtac gttcccgaaa ggctctggtc gttctgctcg
     2881 cgctcctcgg gtgtcaggaa gcgcgtgtgt ccatcgtccc caagcgtatc gagcatccct
     2941 tcgatggccc cgtaggtctc cttcttggaa tctatactct tctggtctac gtagttcttc
     3001 ctgacggtgt cgagcgcctc ggcgtagagt gccacgctct ccctgtcctc ttcgttgagc
     3061 gtggcaggac tctgcgagcg tccgaccatg taagccccga acgctgtcgc gaacaccacg
     3121 acgagcagga agagcacccg cgccaccgag atccgcgtct cagacctggt cttggtctcc
     3181 ttccacacgc ggcaattgta tcttagctag tcagcatgtc agcacgctcc agcctccggc
     3241 ttccgcttgt cagccagtca gcttctcgct tcgggtcttc accctggctg cgcctcgtga
     3301 gacaacctca cagccggggc ctcggtgggt gccaaggagc cacgaaccac gctaccatgc
     3361 tgactagctg aagtgctgac cggctgaaat gctaaagcgc tgaatgccta ttcagcgctg
     3421 taaggcgtgt ttgttgcggg ttcttcgatg cgttggccct cgagcacggg gaagaagcaa
     3481 gcggcctcgt ggccctcctt ctcctgttct tcgaggccgg gctcgtggtt ggtgcagtag
     3541 tcctgggcgc gcgggcagcg ggtgtggaaa gagcatcctt cgggagggtt tgcaggagaa
     3601 ggcacgtctc cggagagcac cacgcgctgg cgctcgcgct ccttttgtgg atcggggacg
     3661 gggatggcag agagcagcga ggcggtgtac ggatggcgcg gctcgccgta gagagcggct
     3721 ctgtcggcta gctctacgat cttgccgagg tacatcacgg cgaccctgtc cgagatgtgt
     3781 ttgaccacgg agaggtcgtg ggctatgaag acgtaggtga ggtcgaactc cttctggagg
     3841 tcctgcagaa ggttgatgac ctgcgcctgg atcgagacgt ccagggccga gacgggctcg
     3901 tcgcagatga tcaggcgcgg gttgagcgcg atggccctcg ccaccccgat cctctgccgc
     3961 tggccgccgg agaactcgtg cgggtagcgg ttgtagtgct cggggttcag gccgaccacc
     4021 tcgagaagct cctgcacccg ccgcttgcgc tcgccggagc tacctcctac gtcgtgggtc
     4081 ctgagcggct cggagacgat attgcccacc gtcatgcggg ggttcaggct cgcgtagggg
     4141 tcctggaaga tgatctgcat gttcctccgc acgccgagca ttcgtttgcg gtcgtagccc
     4201 agtatgtcct cgccgtcgaa gatcacctca ccctccgtgg gctccagaag gcgcaagacg
     4261 aggcgggcga gcgtgctctt gccgcacccc gactctccca cgagcccgag cgtctccccc
     4321 cgacgcacgg agaggtcgac gccgtccacg gccttgacgt gcccgaccgt gcgctgcaac
     4381 acgccggcct ttatggggaa gtgcatcttc aggttcctgg tctccagcag atactcgccg
     4441 gaatggccgt tggtctttgg gctgccgttc gtcccctcgg tcacgtctcg acccccagct
     4501 cctcgtccac gcggctcttg ttctcttcga tctcctcgac ggagaggatg cacgcggcgg
     4561 tgtgggcggg cttcttctcc tcgaggcccg ggtcgatctc gtggcactcg tcctttacga
     4621 aaggacagcg ggggctgaag ttgcacccct tgggaaggaa gatcaagctc ggaggcgtgc
     4681 cttctatcgg aaggagcctc acctcgcccg ccgtatccag gcgcggcagc gagcgcaaca
     4741 gcgaccacga gtaaggcatc aggggatcgt agaagacctc gtctgtggtg gcctgctcga
     4801 cggcgcgacc ggcgtacatc accatcacct tgtccgcgag ttgggagacc acgccgaggt
     4861 cgtgcgtgat cattatgatg gccgtgccgt acttctcctg caactcgacc atgagctcca
     4921 ggatctgggc ctggatcgtc acgtcgaggg ccgtggtcgg ctcgtccgct atgaggactt
     4981 tggggttgca agagaggccc atggcgatca tggcccgctg gcgcatgccg cccgagaact
     5041 ggtgcgggta ctccccggct ctcctctcgg ggttggcgat gccgacgtcg cggagcacct
     5101 gtaccgtctt ctcctgggcc tcgctcttgg agaggttctg gtggatgcgt atggcttcgc
     5161 ggatctggtt tccgacggta aagacgggat tcaggctcgt catcgggtcc tggaagatca
     5221 tggctatgtc gtttccgcgg atctcctgca tccgcttgtc tgaggcctgg agcaggttct
     5281 gatcctcgaa gaggatctca ccttcaggat actcagccgg gggttttgga ttgagctgca
     5341 tgatggagag ggccgtaacg ctcttgccgc tccccgactc ccccactatc cccatcgtct
     5401 ctcccgcctc caacgagaac gagacaccat cgaccgcctt gacgacaccg tcctgggtcc
     5461 tgaaatgggt tttgaggttg ttcacttcca gcatcgccac gggctactcc tcactctccg
     5521 agtggcctct cgccaccaga acggaagggt acagtagtgg cggcgttcgg gccagaggaa
     5581 ccgggttcgt tcaactttgc cccttcatgt actcgaggac agcccggtcg agatagccgc
     5641 gccgctgctc acgctcctcg aagttctcgg tgcggatgta agcgctcagg gcgtcgaaga
     5701 gcgtctcgtc tccgagatct tgcacccgga tgtacccgct gcgacgcagc gcgtcttcta
     5761 cttccccgcg aacctcaccg tctactgcga cgacgtcctc tggcatggtc tcaccgaagt
     5821 agagggtgtg caggtcccgt atacggatca gctcctgtat cgggtcgaga tggtcatcca
     5881 cccgcaggtc cacgaccctg tcgttgtccc cgccgtaccc gccgtcctcc cttacgatga
     5941 gcagcgccgc cgactgcttc ccccgggagt cccccccggc cgcctgcccc gcctccagcg
     6001 ccgagagcag acgcgcggcg aggtcaccgc ccgactcttc gtaagccctc gccatcgcct
     6061 cgaccgtctc cccgccgacg aggatgttgc cctgggccgc gtagtgttcc cctgttatgc
     6121 ctccagccca ctcgaagcac tcggagcccg tgaaggtagc ggccctaccc cgggcgtcga
     6181 cgacaccgac ctgcctatgc tcgcggtcct catcggcgga gatcaactct tcgacggtct
     6241 cataggcggt tttctccctg gccataagct cgagcccgtg tggtccgtag ttgtagttcg
     6301 ccatcgcctg cgtggccacg gcaccaacgc cggcgcgggc ccagggcacg accgagccga
     6361 cggcgaggaa cttggactgg acggcgacgc caagagaatt cgtctcggag tcgaaggcga
     6421 ctatggagaa ggtcgcgacg atctttgtca ctccctgcac acctgctttc tagtcgtagg
     6481 tcctggggtc cagggcttcg cggacaccgt cgccgaggag gttgaagccg aggacggcga
     6541 agaagatggc catgccaggg aagaacgcca tccaccaggc cgtggagagg taggactggg
     6601 cctggaagag catcgagccc cagctcggct ccccgggttc ggcccccagc ccgagatagg
     6661 agagcgtagc ctcggcgatg atcgcgtagc ccattgctag ggtcgtctgg accaggagcg
     6721 gtggaaggga gttcgggagg acgtggcgca gcatgatcat cgtgtttccg gtgccggtcg
     6781 ccctggcggc gtcgacgtag tcgaggcccc tgaccgtgag gacctgggcg cgcgttatcc
     6841 tcacgaaagc cgggatgaag cctatgccta tggcgatcat ggcgtacccc aggcccccgc
     6901 cgaggatcgc cgctataacg agggccagga taaggaaggg gaaagcctgc aggctgtcca
     6961 cgacgcgcat cacgatccac tcgtcccaaa atcccctgta gtatccgctt gtcaggccga
     7021 cgggcacacc gacgaggagt gctatcccca cggagatcag ggaagattcg agcgagatcc
     7081 tggctccgta gaccaccctg gagaacacgt cgctgcccag agaatcggtc cccatcaggc
     7141 cgccttcccc cggcggcctc agggtgaggg cgtagtcctg agcgctggga tccgatggcg
     7201 cgatcaaagg cgcgaagatg gccgtaaaga gtaacaaggc cacgatgagg agcccgaaaa
     7261 cggcgagcct gttccgcctg aagcgtcgcc agaacgcggc caaacgccgc tcccgcggct
     7321 ctaccgcctg cgtctgcgct ccctcggcgg ttaccgcctc gctcatccct actccccgct
     7381 actcaccgag atgcgcgggt ctatgatcgt gtagagaacg tcgaccagga cattcacgag
     7441 cacgacgatt atcgtgatgg taagcacggc cccctggacg gtggtgaagt ccctctgctc
     7501 gatagaatcg acgaccaggc gtccgagacc agggagctgg aagaccgtct cggtcaccac
     7561 aagccctccc agaagacccg ctatctgcag gccactcgcc gttacgaacg gtatcagagc
     7621 gttcctgacg gcgtgccgga tcaccacgat ccgcttggac agtcccttgg agaaggccgt
     7681 gcggacgtag tcggagccca gctcctccag caggctgctc ctgagcatcc gcgtaagctc
     7741 cgccgactcg cgcagtcccg tggccaggat cggcaggatc atggcggtga tgttggcgac
     7801 ggggttctgg aagaacggtt cgtagcccga ggcgggcaac cagcccaaac tgaccgcgaa
     7861 gatgataata aacatcatcc ccagccagaa atgcggtatg gagatgccgc ccagggccac
     7921 gccagtgctc aggtagtcca cccacgtgcc cctgcggctc gccgagagga tgccggcgac
     7981 caccgcgata gtcaggctca cggcgaacgt accgacggcc agctcgatcg tcacgggcag
     8041 gcgctccagg ataagctgcg acactggtgt atcgtcgatg aggaactcgc ctagatctcc
     8101 cttcagcacg cctctcagcc agtcgaa
//
LOCUS       DATMVF010000159        18985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_485625, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000159 DATMVF010000000
VERSION     DATMVF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18985
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485625"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VN178_08005"
     CDS             complement(<1..996)
                     /locus_tag="VN178_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWS80954.1"
                     /translation="MKRSKSADPAHDVLGYERQQLDAIFRPETVAVIGATERPGSVGR
                     TIMWNLVSSPFGGTVFPVNSRRPNVLGIKAYPSVSEVPARVDLAVIVAPAPAVPEIIR
                     ECAEAEVGGAVIISAGFRETGSEGVELERQVLEEARRGRMRIVGPNCLGVMKPPTGLN
                     ATFAGAIARPGNVALLSQSGALLTAILDQSFMENIGFSALVSTGSMMDVGWGDLIYYL
                     GDDPQTKSIVIYMESVGDARSFLSAAREVALTKPIIVIKAGRTEAAGKAAASHTGSLT
                     GSDEVLDAAFSRSGVLRVNEISELFGMAEVLGKQPHPKGPRLTILTNAGGPAVLAT"
     gene            1461..2321
                     /locus_tag="VN178_08010"
     CDS             1461..2321
                     /locus_tag="VN178_08010"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWS80955.1"
                     /translation="MRREGLTRTFAGDPLLKVGAGAMILAVGFAIVVAFAAYLDRPIE
                     PAAAKPEVKSATEPLARSDPGVDPWVEKDIPPSRFRATPVPANPSESDRPPDPRPGPP
                     AGSGSAYGSSPDQSRQTISPNGYDYPLPTDGQLRAANRPRHYNLPPGAVMSLTISALE
                     LRNVPVISSDATQALDQGVIHLPGTSYPWSGTPERNVYLAGHRLGWPGTGSHLVFYRL
                     NELGGGERVTLRDARGGRYHYRVIESFVVRAGESWVTGRVRGRDLLTLQTCTPIPTFE
                     KRLIVRAERI"
     gene            complement(2516..3541)
                     /locus_tag="VN178_08015"
     CDS             complement(2516..3541)
                     /locus_tag="VN178_08015"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWS80956.1"
                     /translation="MDVQKLVETARELVAPGKGILAADESFGTIGKRFEAVGIESSEE
                     SRRAYREMLFTTEGIGEFLSGVILFDETIRQETSDGTPMPQALEDAGVIPGIKVDKST
                     VDLPLSEGEKFTQGLDGLGERLPEYTELGARFTKWRAVITIGDGIPTESCIGANAEAL
                     ALYAAFAQEHGLVPIVEPEVLIDGDHSIEQCYDATEWMLHRTFGELYEHGVELSGILL
                     KPNMVISGNDAPEQSSVEEVATATVECLLRTVPAAVPGIVFLSGGQSDLQATAHLNAM
                     NSLYGDLPWELSFSYARALQGQPMEIWGGDVDKVQAAQKAFYHRAKMNSAARSGDYSE
                     EMEEQAA"
     gene            complement(3629..3787)
                     /locus_tag="VN178_08020"
     CDS             complement(3629..3787)
                     /locus_tag="VN178_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80957.1"
                     /translation="MSKEAVAMCYSYRDRRMEEEAHRKQWEKEGRRRREQQEKERQAK
                     KDRELVKA"
     gene            4006..6057
                     /locus_tag="VN178_08025"
     CDS             4006..6057
                     /locus_tag="VN178_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWS80958.1"
                     /translation="MANRLASETSPYLLQHKDNPVDWYPWGEEALKRAREEDKPVLLS
                     VGYSACHWCHVMERESFEDDETARMMNEHFVNVKVDREERPDIDSIYMAAVQALTRHG
                     GWPMTVFLTPDGAPFYGGTYFPPIPSRGMPSFQQVLLSVVDAYENRREEVLQNARAVR
                     DYLQASTAAVMPEAESAGTELLDRAASALLSQLDRRFGGFGGAPKFPQAMNLEVLLRH
                     YHRTGERSALDGVELTCRQMANGGIYDQLGGGFSRYSVDAYWLVPHFEKMLYDNALLS
                     RLYLETYQATGDAFYRRIAEETLDYVLRDMTSEEGGFYSAEDADSEGEEGKFYVWTPD
                     ELAAALDPDEASLAARYWDVTERGNFEGKNILHVARPPEAIADEFGLSTEELWARIAA
                     IRTKLSAVREERIRPGRDEKILAAWNGLMLRSFALAARVTGREDYREAALKNATFIVE
                     KLKEDGRIHRSYKGGRARFNGYLEDYAMVADGLVALYEATFETRWLIEADALLDTVSE
                     LFWDEGKRVFYDTPADHEELVTRPRDVYDNASPSGTSVATEVLLKLALFLDRGDYRQR
                     AEDVLEELAGGMEKVPGGFGRLLCALDLSTSETREVAIIGDPEATDTRDLLEAVYSGY
                     LPNKVVAGRGPDNDEAGGLIPLLAQRPAREDKATAYVCVHYACQTPTTDPEELSRQLG
                     A"
     gene            complement(6496..7149)
                     /locus_tag="VN178_08030"
     CDS             complement(6496..7149)
                     /locus_tag="VN178_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS80959.1"
                     /translation="METIIPGLHASVPEPLGFGPSLEIRAFLLQRERGNLLIYRAATL
                     EQDVEAVAGLGGVSRQYLNHYHEASPACDWVASTFHAPLYVHEADARAASEVCNVDET
                     FSERHKLDEDLEIIPIPGHTSGATAFLWDTGQHRALFTGDTVFFIRGRWRAAVLDGVS
                     DRGRYIESLELIRTLDFDIVVPGIAPAGEPYYELVEKPEAEHRIDEILERLRRGESG"
     gene            complement(7216..7566)
                     /locus_tag="VN178_08035"
     CDS             complement(7216..7566)
                     /locus_tag="VN178_08035"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HWS80960.1"
                     /translation="MDHAPPTRVEREVASWPGVSSGDTGRGGLQLSYGKVELGHLHGS
                     SFADLPFPKKIRDELIAEGRASVHPPLPDSGWVRRRMDGPGDAEAVIELFRMNYERAR
                     TRAERRAPSESKER"
     gene            complement(7715..8602)
                     /locus_tag="VN178_08040"
     CDS             complement(7715..8602)
                     /locus_tag="VN178_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80961.1"
                     /translation="MLFPDGKLPSKRWRPLAWFSVVVIVLLSVAGGFAPGPLENQGGV
                     RNPFGLEALPWLQDAFYLILPLLLLCIFASAVSMVLRYRRSRGEVRQQIKWVAFVASF
                     SGLLYLIPMILASIVQLRSDDRLPQLPWWAEVFFAVAVLGFAGVPVAIGFAVLKYRLY
                     DIDVVINRTLVYGSLTAMLVALYFGSVATTQTILRALTGQTEQPQLAVVISTLVIAAL
                     FNPLRRRIQSFIDRRFYRRKYDARKTLEAFSARLRDETNLEMLNNELTGVIRETMQPG
                     HVSLWLHPDRPPKQGQAER"
     gene            8697..9574
                     /locus_tag="VN178_08045"
                     /pseudo
     CDS             8697..9574
                     /locus_tag="VN178_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(9606..10433)
                     /locus_tag="VN178_08050"
     CDS             complement(9606..10433)
                     /locus_tag="VN178_08050"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWS80962.1"
                     /translation="MDEALDPFRITDKLEDVLLDVMVSRLEVRGNHPFFQKVLREYLE
                     AMGIDAATTVLDMGCGTGVAARTIARRKNFSGMVTGIDLSPYLVNAAERLADEEGLGD
                     RVEFRSGDTRDLDIPDGNFDAVVAHTLVSHVGEVLGVLEEAGRVLKPGGLIAIFDGDY
                     ASMTFALEDPVESKRYDEALIDALVTNPRVMRQMPRLIRGAGLEMLSTFPYVMAEIGE
                     GDFWLSAVDAYEKLAPKSGAISEAEAADWARALRSASDENVFFGASGYYTYIARRPG"
     gene            complement(10605..10997)
                     /locus_tag="VN178_08055"
     CDS             complement(10605..10997)
                     /locus_tag="VN178_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80963.1"
                     /translation="MSARAAARLAWSLAALSVAMFVASGVLYVLAEAAQATGDWVTVG
                     PVSETVTFLAFLAFPIVGALVASRRPENPIGWICLANGLLWMLLSVSDGYSVYGVARP
                     GSVPFPVAVGTLSNQWLWVPTLGLGPVW"
     gene            complement(11190..12122)
                     /locus_tag="VN178_08060"
     CDS             complement(11190..12122)
                     /locus_tag="VN178_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWS80964.1"
                     /translation="MPKTHYFPEDWVHYVWDNEQEPVLTVEPGDTVVYHTREVSDGQI
                     GPDSTVDALSSLDWDSLYPLAGPVAMADAEPGDMLAVEVLDMHTAGWGWTAVLPGFGL
                     LPEDFPDAYLKIFDLTNGDYTLFKDDIAIPIEPFFGTMGVSPSGPEKPVIMPPGPFGG
                     NMDTRHITKGTVLYLPVQHSGALFSCGDAHAAQGDGEVCVTGIECPMYAALRFDLIKG
                     RSIPAPQFQTPGPLTPSVDEAGWYATMGVNEDLMQAAKDSLRAMVEHISATYGLEPVE
                     AYVLASLVVDLKISEIVDQPNWIVSSYLPLAIFG"
     gene            12480..12950
                     /locus_tag="VN178_08065"
     CDS             12480..12950
                     /locus_tag="VN178_08065"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HWS80965.1"
                     /translation="MRRLYVLTASAVIATLLIALTMSTVSAQGVSKRHGAIAAVPSLV
                     RGIDGYGATLKQAKSAAIEECRKVASHRRKYAEDCQGAVWVRNGWIAIAFERTVEKPY
                     RDLAWGSGWGHTKDSAKYQARTVCRQYAEEKCEVKSTYETPVRDPSLGSKGGSW"
     gene            13082..14047
                     /locus_tag="VN178_08070"
     CDS             13082..14047
                     /locus_tag="VN178_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80966.1"
                     /translation="MTDDRTGYKRYLSQRYLASLPERTARAGAALTGGLVYETGEVLL
                     PLAVRRSRLYQAVVGRLLRIMIELVGGVEGVYPTEEMPVRELLVRKSAGNVIELSSFL
                     AVGWSPVWLLAGASDLVGGTKVYLRTLVTELRDAGVLAPDADVASFEELLTTLEGTSG
                     VLADTVDVPPLNISSVRTSWQQLRLQVADLPDAEGLEKIFSDLQLAARQEDRSVLEIS
                     SMVALGAVHAGVRLGDAHIFDYYRNALPTIVEEGLVSFLRRVSTPYLTRARSHYDPGS
                     STYSERLLRRWADRRSSVARQMTTQDAASSKENAPEDYSTGEGQD"
     gene            14246..14608
                     /locus_tag="VN178_08075"
     CDS             14246..14608
                     /locus_tag="VN178_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80967.1"
                     /translation="MATVEDLDGVIERCQQAMREFGKGNPEPMKGMYSRREDVTINTA
                     IDPPARGWDEVALRMERSASGFREGEIDAFETVTRVVTPELAYVVWVERHRSKIGERD
                     EMVDFPLRVTIIFRLEDG"
     gene            complement(14810..16033)
                     /locus_tag="VN178_08080"
     CDS             complement(14810..16033)
                     /locus_tag="VN178_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HWS80968.1"
                     /translation="MKHPRRFETRAVHSGEHRAGAKEGRDGIFYPISTPIYASTTFSH
                     PNIETTDRVLGGDERGYSYARYDNPTVRAFEEALVSLEAPEAEGDVRAFAFASGMAAT
                     HAALAAAELTGGGTVLAADQLYGSTVTLLVQIFGASGVETRFVDAYDLGAVEKKVGEL
                     KPRAVVVESISNPLLRVADVPEIAKITRAAGAALIVDNTFGTPYLQRPLELGADIVVH
                     SATKYLSGHGDLTAGAVVAGPPYAAAVEQLRKLVGGVLGPFEAWLAHRGLKTLPLRMG
                     RQCENARQIASSLAAHPKVAKVNHPSLGAHPDHETAGRVLSDTGGLVSFELAAEGREA
                     AFAYLNALELCVKAPSLGDIYTLAIHPATSSHRELSPSRRQRLGVRENLVRLSVGIEH
                     AGDVIFDLEQAFERV"
     gene            complement(16045..16230)
                     /locus_tag="VN178_08085"
     CDS             complement(16045..16230)
                     /locus_tag="VN178_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80969.1"
                     /translation="MRPVPEMGDYRSPVENVYLCGSGSHRGPGVSMAPGRNAAETILS
                     DLGLDFGAVVATGATRS"
     gene            complement(16636..16953)
                     /locus_tag="VN178_08090"
     CDS             complement(16636..16953)
                     /locus_tag="VN178_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS80970.1"
                     /translation="MEDRIDYFERMLADNPDNPTGLLALANEYEKAGRDEDEAAVLER
                     YLAVHVDDEGNAYARLGSVLSKLGRKDEARAAYEKGIAQSEKHGHSGMADDLRLALIQ
                     LGE"
     gene            17286..>18985
                     /locus_tag="VN178_08095"
     CDS             17286..>18985
                     /locus_tag="VN178_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWS80971.1"
                     /translation="MSREGVIRLERETENQFFGPNLGYVLELYENYREDPDSVDGQMR
                     EFFESWSPPRVGTPGANGQQVAPEVDVEKAVGASKHVRHIRDFGHRAARLDPLGSEPP
                     GDPTLDPTFYRITNEDLETLPSLIIGGPIAERTSNAGEAAEELRRIYCDTTGYDFGHV
                     HDPEERFWLRESVESGRFEKRMDGENAMRLLERLTRVDAFEKFLHKTFLGQKRFSVEG
                     NDMVVPMLNRIIRSAADVGTPVVTLGMAHRGRLNVLAHVMDKPYSKIFGEFQQPEKGE
                     QSSAASSPGAAWVGDVKYHLGIRGFQLADEEADEEVFIHLAPNPSHLEHVNPVVEGMA
                     RASQEELDEAGPPQQDVDASLPILLHGDAAFPGEGVSAETLNLSRLPGYRTGGTIHII
                     TNNQLGFTTERQDARSTTYASDLAKGFEIPVVHVNADDPEACMAVARMAHAYREKFNK
                     DFMIDLVGYRRFGHNEGDEPAYTQPLMYETIRDHPTVREIWAQELERRGILDEGEAEA
                     MFEEIMSAMAEVQKKATDELKEEDFATDELYTPLVGIPETAVAAERLSELNEALLEVP
                     "
BASE COUNT         3589 a         5990 c         5953 g         3453 t
ORIGIN      
        1 cgtcgcgagc accgcgggac cgccggcgtt cgtaaggatc gtgaggcgcg gtcctttagg
       61 gtgcggttgt ttgccaagca cctcggccat cccgaaaagc tcggagatct cgttcacgcg
      121 cagcacgccg ctgcgcgaga aggccgcgtc gagcacctca tccgagccgg tcagggagcc
      181 tgtatgggag gccgccgcct tacccgcggc ctcggttcgt ccggctttga tcactattat
      241 cggtttggtc agggctactt cgcgggccgc cgagaggaat gagcgggcgt cgccgaccga
      301 ctccatgtag atgacgatgc tcttcgtttg ggggtcgtcg ccgaggtagt agatcaggtc
      361 tccccaacct acgtccatca tggagcccgt gctgacgagc gccgagaagc cgatgttctc
      421 cataaagctc tggtccagga tggccgtgag caaagcccca ctctggctta ggagcgccac
      481 gtttccggga cgggcgatgg cgccggcgaa ggtggcgttg aggcccgtag gcggcttcat
      541 gacgccgagg cagttgggac cgacgatcct catcctgccc ctcctggcct cctcgaggac
      601 ctggcgctcg agctccaccc cctcggaccc agtctccctg aaaccagccg agatgatcac
      661 ggcgccaccg acctcagcct cggcgcactc acggatgatc tccggcaccg cgggcgcagg
      721 ggcgacgatg accgcgagat ccaccctggc cggcacctcg gagaccgaag ggtatgcctt
      781 tatacctagt acgttgggcc tgcgcgagtt cacgggaaag accgtcccgc cgaacgggct
      841 gctcacgagg ttccacatga tcgtgcgccc cacgctacca ggacgctcgg tcgccccgat
      901 cacagccacc gtctcggggc ggaagatggc atccaactgc tgccgttcgt aacccagaac
      961 gtcgtgggcc gggtccgcgc tcttggaccg cttcattagt cgctcccttc ggtggctcca
     1021 gctagtcagc aagtcagcac gctcaggctg acgccttcgc tcgtcagcat ttcatctttt
     1081 gtaacaccgg gcgtaagatt gagtcgtgaa ctcgcctacg gaaggccctg agcgggacgt
     1141 ttttggctga cgcctgagtc cgcttgagaa cggcccgtgc ggtcgtatcc ttcgctctcg
     1201 cgctcgagga tgtagaacgg gaaacccagg cggtatatga tgcggtgtgc atcgcaacgt
     1261 tctgagggta cccgagcaca gcctcacggg ttgatctcgg agcacgatgg atggggagag
     1321 taccatgggt aacagggggc tcggtaacgc cggcggcaga caactgtggg gagcctggcg
     1381 cggtccttct acgccatgtt ggcgacagga ctactgccgg tggggcgacc ctcaggcgcg
     1441 gcaggtaggg cgactctgct ttgcgacgtg agggactaac ccgcactttc gcgggggacc
     1501 cgctcctcaa ggtcggggcg ggcgcgatga tcctcgccgt gggtttcgct atcgtggtgg
     1561 ccttcgccgc ctacctcgac cggccgatag agcctgcggc cgcgaaaccg gaggtgaagt
     1621 ccgccaccga gccactcgcc cgctcagacc ccggcgtcga cccgtgggta gagaaggaca
     1681 tacctccgtc caggttccgg gcaacacccg tgccagccaa cccctcggag tcggatagac
     1741 cgccggatcc cagaccaggc ccaccagcgg ggagcgggtc tgcatacggg tcatccccgg
     1801 atcaaagccg ccagaccatc tccccgaatg ggtacgatta tcctctgccg acggacgggc
     1861 aactcagggc tgccaaccga ccaaggcact acaatctgcc acctggggcc gtcatgagcc
     1921 taaccataag cgccctagag ctaaggaacg tccccgtcat ctcctcagat gctacccaag
     1981 ccctggatca aggggtgatc cacctgccgg gcacttccta tccctggtca ggtaccccgg
     2041 agaggaacgt gtacctggcg ggacacaggc tgggatggcc tgggacggga agccacctcg
     2101 tcttctaccg cctcaacgag ctgggaggag gggagagggt aacattgagg gacgctcgag
     2161 gcggcagata ccactacagg gtgatagaga gctttgtggt aagggccggg gagagttggg
     2221 taacggggag agttaggggc agggacctct tgacgttaca gacgtgcacg ccgatcccga
     2281 ccttcgagaa gcgcctgatc gtacgcgccg aaaggattta gagctatcag gcatcagcct
     2341 tcagcgatca gcttctctgc ctgattggcc cgacgttacg gccgctcgca tagcagcgag
     2401 ctccaaccac cgtgcgcttg ggccacccgc tgctagcgcg atggcaagct ttcgcccaga
     2461 ttagaaggct gatagctgac cgctgaaagc taacagcgcc tcccgaaggg aggcctcacg
     2521 ctgcttgttc ttccatctcc tccgagtagt cgcctgagcg tgcggcgcta ttcatcttcg
     2581 ccctgtggta gaaagccttc tgggccgcct ggaccttatc tacgtctccg ccccagatct
     2641 ccattggctg tccctgcagc gcccgtgcgt aggagaacga gagctcccac ggcagatccc
     2701 cgtataggga gttcatggcg ttgaggtggg ccgtagcttg caggtcgctc tggccgccgg
     2761 agaggaagac tatgcccggc accgccgccg gcaccgtcct gagcaggcac tccaccgtgg
     2821 ccgttgctac ctcttccacg ctggactgtt ctggcgcgtc attgccggag atgaccatat
     2881 tgggcttcag cagtatcccc gatagttcta cgccgtgctc gtagagctcc ccgaaggtcc
     2941 tatggagcat ccactctgtg gcgtcgtagc actgctcgat ggagtgatct ccgtcgataa
     3001 ggacctcagg ctcgactatg gggacgaggc cgtgctcctg ggcgaacgcc gcatacaggg
     3061 cgagcgcctc agcgttggcc cctatgcagc tctcggtggg gatgccatcc cctatggtta
     3121 tgactgcccg ccacttggtg aagcgcgccc cgagctcggt gtattcgggg agcctctcgc
     3181 caagtccgtc gagaccctgg gtgaacttct cgccttccga gaggggcaaa tcgacggtgc
     3241 tcttatccac cttgatacca gggatgacgc cggcgtcctc gagcgcttgg ggcatggggg
     3301 tgccgtccga ggtctcctgg cggatggtct cgtcgaagag gatcacaccg gagagaaact
     3361 ccccgatgcc ctccgtggtg aagagcatct cccggtaggc ccgacggctc tcctcgctcg
     3421 actctatgcc gactgcctcg aaccgcttgc ctatggtgcc gaagctctcg tcggcggcga
     3481 gtatgccttt ccccggagct actagttcgc gggccgtctc tacgagcttc tgaacgtcca
     3541 tcactgatct ccttccctgg gaccttacga tagaggcccc ggaccgcggt ccggggcccg
     3601 tgatcatgga gtttttggag gtcctttcct aggcctttac caactcgcga tctttcttcg
     3661 cctggcgctc cttctcctgc tgttcccggc gcctgcgtcc ttctttctcc cactgctttc
     3721 tgtgggcctc ttcttccatc cggcgatccc ggtaggagta gcacatggcc actgcctcct
     3781 tgctcacgtt tccctatccg tattctgtct acccgcatct tgacgggcta aacattgccc
     3841 acacgggaaa aaacaacgcc gtgtccgagc cctcgtttca tacccggcgc gcggacgcca
     3901 ccaacggccg gggctacgga cccgagggag gcgcaggata ccgaacctcc gatccgtgat
     3961 acgttagagt aagcggctag accccttttc gggaggtgag aggctgtggc caacaggctg
     4021 gcgagtgaga cgagcccgta tctgctccag cacaaggaca accccgtgga ctggtaccca
     4081 tggggcgagg aggcattgaa gagggcgcgg gaggaagaca aacctgtcct gctctcggtc
     4141 ggctacagcg cgtgccactg gtgccacgtc atggagcgcg agtccttcga agacgatgag
     4201 accgcgcgca tgatgaacga gcacttcgtg aacgtaaaag tagaccgcga ggaaagaccc
     4261 gatatagact ccatctacat ggccgccgtc caggccctca cccggcacgg cggttggccg
     4321 atgaccgtct tcctcacccc cgacggcgcc ccattctacg gcggcacgta cttcccgccc
     4381 atcccctcgc gcggcatgcc ctccttccag caggtcctcc tcagcgtcgt cgacgcctac
     4441 gaaaaccggc gtgaagaggt cctgcaaaac gcccgggccg tacgcgacta cctccaggcc
     4501 tcgacggccg ccgtcatgcc cgaggcagag tctgcgggaa ccgagctgct cgaccgcgcg
     4561 gcaagcgccc tcctctccca gctcgacagg cgctttggcg gcttcggcgg cgcccccaag
     4621 ttcccccagg cgatgaacct ggaggtcctg ctccgccact accaccgcac gggagagagg
     4681 tccgcgctcg acggcgtgga gctcacgtgc cggcagatgg cgaacggtgg catctacgac
     4741 cagctcggcg ggggcttctc ccgctactcc gtggacgctt actggctcgt cccgcacttc
     4801 gaaaaaatgc tctacgacaa cgccctcctc tccaggctct acctcgagac gtaccaggcc
     4861 acaggagacg ctttctaccg ccgcatcgcc gaggagacgc tcgactacgt cctgcgcgac
     4921 atgacgagcg aggagggggg tttctactcc gccgaggacg ccgactccga gggcgaggag
     4981 ggcaagttct acgtttggac gcccgatgaa ctcgcggccg cactcgaccc cgacgaagcg
     5041 agcctcgcgg cacgctactg ggacgtcacc gagcggggca acttcgaggg caagaatatc
     5101 cttcacgtcg cccgtccccc cgaggctatc gccgatgagt tcgggctctc cacggaggag
     5161 ctgtgggccc gcatagctgc gatccggaca aagctatccg ccgtgcgtga ggagcgtatc
     5221 aggccggggc gcgacgagaa gatcctggcc gcctggaacg gcctcatgct ccgctccttc
     5281 gccctcgccg cccgcgtcac gggtcgcgag gactaccgcg aagccgctct gaagaacgca
     5341 acctttatcg tggagaagct caaggaagac ggccgcatcc accgctctta caagggtggt
     5401 cgggcgaggt tcaacggcta cctggaggac tacgcgatgg tcgctgacgg gctcgtcgcc
     5461 ctctacgagg ccaccttcga gacgcgctgg ctcatcgagg ccgacgccct cctcgacacc
     5521 gtctcagaac tattctggga cgaagggaag cgtgtcttct acgacacccc ggccgaccac
     5581 gaggagctcg tcacccgccc gcgcgacgtc tacgacaacg cctcaccctc aggaacctcc
     5641 gtcgccaccg aggtcctact caagctggcc ctcttcctcg acaggggcga ctaccgccag
     5701 cgcgccgagg acgtcctaga agagctcgcc ggcggtatgg agaaggtccc tggcggattc
     5761 ggtcgtctcc tctgtgccct cgacctgtcc acctcggaga cccgcgaggt agccataatc
     5821 ggagacccgg aagcaacgga tacgagagac ctgttggagg cggtctactc tggctatctg
     5881 cccaacaagg tcgtcgcggg ccgcggcccc gataacgacg aggcaggagg cctgatcccc
     5941 ctcctcgccc aacgccccgc ccgcgaagac aaagccacgg cttacgtctg cgtccactac
     6001 gcctgccaga ccccgaccac cgaccccgaa gagctctcgc gccagctcgg cgcctgacaa
     6061 gtgctgcctg aacaaggtat ggttacataa ggtatgcgaa agacgactgt gtacttgaat
     6121 gaagaagtcg aaggactccg tctactggcg agcgtgacgg gtgagtctcg agctcagttg
     6181 atccgtaaag gtgttcgccg gttgctgcgg agggtgtaag ccgcaagttc cactgcatgg
     6241 agaagggtga gggaccgggt gagccccgtc cccggtggag tcctgacgtc gtgcgcgaga
     6301 agacgctcgg acgcagctag ggttcattgg cgttgatcgt cggcgcccga ggattatacg
     6361 cccaggcgga ccgtgcgggc ccggcgcaca aagccgtggt cgagagcttc accgtcctcc
     6421 cggtagacgc cggatcggaa gattcagcgt aggcggagaa gactatcggt cggaggagct
     6481 tcatgatccg gccgctcagc cgctctcgcc gcgtcgtagc cgctcgagga tctcgtctat
     6541 gcgatgttcg gcttccggct tctctacgag ctcgtagtaa ggttcgcccg ctggtgcgat
     6601 ccccggcacg acgatgtcga aatcgagggt gcggatcagc tccaggctct ctatgtagcg
     6661 cccccggtcg ctgaccccat cgagtaccgc cgccctccac ctgccccgga taaagaagac
     6721 cgtgtcgccg gtgaacaggg cgcgatgctg ccccgtgtcc cacaggaaag ccgttgcccc
     6781 gctcgtatgt cccggaatgg gaatgatctc caagtcctca tcgagcttgt ggcgctcgga
     6841 gaaggtctca tccacgttgc agacttcgga ggccgcccgc gcatccgcct cgtggacgta
     6901 aagcggcgcg tggaaggtgc tcgcaaccca atcgcaggcg ggcgaggcct cgtgatagtg
     6961 gttcaggtac tggcgtgaga caccaccgag gccggctacg gcctctacgt cctgctcgag
     7021 ggtggcggcc ctgtagatga gcaggttgcc cctctcgcgc tgtagcagga aagcgcggat
     7081 ctccagcgaa gggccgaacc ccaaaggctc gggcacagaa gcgtgaagcc cgggtatgat
     7141 ggtttccatt gctcgttcct ttcagggtga tcggcgatgg gcgttgtgtt cttcttgggt
     7201 actaccccta gaggatcatc tctctttaga ctctgagggc gcgcgacgtt cggcgcgggt
     7261 ccgtgcgcgt tcgtagttca tgcggaagag ctcgatcacg gcttccgcgt cgccgggacc
     7321 gtccatcctc cggcgtaccc aacccgagtc gggcagcggc gggtgcaccg aggctcgtcc
     7381 ttccgcgatc agctcgtcac gtatcttctt gggaaagggc aggtcggcga agctcgatcc
     7441 gtgcagatgc cccagctcca ccttgccgta actgagctga agcccacccc tgccggtgtc
     7501 gccggaactg acccccggcc agctcgcaac ctcacgctct acccgcgttg gcggtgcgtg
     7561 gtccatcagt tccctccttc tatgagcctg cctcacacga ctttatagtg cgggaaggga
     7621 cctgatgcat cgtgcgcagg aacggttttc ggcctggacc ttcgtacagg ctatgcgcga
     7681 cggtctgcgg gcgggcaccc cgccggatag agcgctaccg ttcggcttgc ccttgcttgg
     7741 gaggcctgtc ggggtgcaac catagcgaaa cgtggccagg ctgcatcgtc tcccttatta
     7801 ctcctgtaag ttcgttgttc agcatctcga ggttcgtctc gtcgcgaagc ctggcggaga
     7861 acgcttcaag ggtctttctc gcgtcgtact tgcgcctgta gaaacgacgg tcgataaagg
     7921 attggatgcg gcgcctaagc gggttgaaca gcgcggcgat cactagggtc gagatgacca
     7981 cggccagttg cggttgctcg gtctggcccg tgagagcacg gaggatcgtc tgggtcgtcg
     8041 ccacgcttcc gaagtagagt gcgaccagca tggccgtgag tgaaccgtag actaacgtgc
     8101 ggtttatgac gacgtctatg tcgtagagtc ggtacttgag gacggcgaag cctatggcca
     8161 cgggcactcc cgcgaagcca agcacagcca cagcgaagaa gacctcggcc caccaaggaa
     8221 gctgcggtag gcgatcatcg ctcctcaatt ggacgatcga tgcgaggatc atggggatca
     8281 ggtacaacag gcccgagaat gaggcgacga aggcaaccca tttgatctgc tgacgcactt
     8341 cgcccctcga acgccggtaa cgcaacacca tgctcaccgc cgaagcgaag atgcacagga
     8401 gcagcagcgg gagaatgaga tagaacgcat cctgcagcca ggggagcgcc tcgagcccga
     8461 acgggttgcg tacccctccc tggttctcta aaggcccggg ggcgaacccc ccggcgacgc
     8521 tgagcaacac gatcaccact accgagaacc aggccagcgg acgccatctc ttggagggga
     8581 gcttcccgtc cgggaacagc aagatgaggt agatcccgag tagagcgtgt ctgacgaatg
     8641 gcgagccccg tggtatccct gctcggatag gacaagcgac cacgggaggg actaccatgg
     8701 tcaggcgcgg ggagatcacc gaccgggctt gggagcagat agagctgctc ctgccccgag
     8761 agggcggcaa gagaggcggt cagtggcgcg accatcgcac cgtcatcaac ggcatcctct
     8821 ggaagctcag gaccggatcg ccctggcgcg acctgccaga aaagtacggt ccctggcaga
     8881 cctgcttcga ccgcttcaac cgctggaggc gcgacggtac ctggaatcgc ctcctgacgc
     8941 acgctcagac caagagcgac gcggtcggcg acgtgcggtg ggaggtgagc gtagacgaca
     9001 cggtcgtccg ggcccatcag catgccgctg gcgccagacg cgagcccgcc aagaacgagg
     9061 acgtaaaagg gggcccagga gccccgaagg tgaggctttg ggacgctcga gaggcgggtt
     9121 ctccaccaag gtgcaactcg cttgcgacgg gaagggtcgt ccgctatccg tggtcgtcac
     9181 cactggggag cgtcatggaa gtacgcagct cgagccactc ctcgacgagg tacgcgtgcc
     9241 gcgttctgag ggcttaccgg gccgaccgcg caagcgtccc gctcacctga tcgccgaccg
     9301 gggctacagc ttcgagagtt gtcgtaggtt gctacgcagg cggggcattt cccacactat
     9361 ccccgagcgc aaagaccaac gcgagcgtcg caccggacgt cccggtcggc gtccgggctt
     9421 cgaccgagag gcttaccgca ggcgcaacgt ggtcgagagg tgtgtgggcc gtctcaagca
     9481 gtggcgcagc gtagcgacgc gctacgagaa gctggcggtc aactaccgaa cgatggtggt
     9541 aatcgcctcg ctgatggtgt ggctgccttc gtgaatcatc gggtgcgatc tagcactcgg
     9601 agatgctacc cggggcgcct cgcaatatag gtatagtaac cgctcgcacc gaagaatacg
     9661 ttctcgtcgg acgcgctgcg cagcgcacgg gcccaatcgg cagcctctgc ctcgctaatc
     9721 gctcccgact tgggtgcgag tttctcgtac gcatcaacag cggaaagcca gaagtcgccc
     9781 tctccaatct ccgccattac gtaggggaac gtgctgagca tctccagtcc ggctccccga
     9841 ataaggcgtg gcatttgccg catgacccta gggttcgtca cgagcgcatc gatcagggcc
     9901 tcatcatacc tcttgctctc taccggatct tccagggcaa acgtcatcga tgcgtaatcc
     9961 ccatcaaaga tggcgatcag tccgccgggc ttgaggactc tgcctgcttc ctcgagcact
    10021 cctagcacct ctcccacgtg gctgacgagc gtgtgcgcta ccacggcatc gaaattgcca
    10081 tcggggatat cgagatctcg tgtatccccg gagcggaact cgactcggtc gcccaaccct
    10141 tcttcgtccg ccagtcgctc tgcagcgttc accagatagg ggctgagatc tatccccgta
    10201 accatgcccg agaaattctt tcgacgagca atcgtacgcg ccgccactcc cgttccgcag
    10261 cccatatcga gcacagtggt tgccgcatcg atgcccatcg cttccaggta ctctcgcaac
    10321 accttctgga agaaagggtg gtttcctcga acctcgagcc gtgagaccat gacatcgagg
    10381 agtacatcct ctagtttgtc ggttatccga aagggatcta acgcctcatc cacgtgccct
    10441 cctcgagtca accgcccggt agttgcgccg ctccatgcac ataacgtccc acgcgaagca
    10501 attgtaacgc tatgcgcaac aagggagctc gggtacggct ataggccgat cgccttcctt
    10561 ggtccagcct ctccagccaa ggagtccacg ggagctaaaa cggttcacca gacaggccct
    10621 agtcccaagg tgggcaccca cagccactgg ttgcttagcg tgccgaccgc cacaggaaag
    10681 gggaccgagc ctggtctggc aacgccgtat acgctgtagc cgtcgctgac cgagagcagc
    10741 atccacaaaa ggccgttcgc caggcagatc caacctatgg ggttctcggg acgcctggag
    10801 gcgacaaggg cgcctacgat tgggaaggcc aggaaggcca ggaaggtcac cgtctcgcta
    10861 acggggccga cagtgaccca gtccccagtg gcctgcgcgg cctcggcgag gacgtagagc
    10921 acgccgctgg cgacgaacat ggccaccgag agcgcggcca acgaccacgc cagccgtgcg
    10981 gcggcgcggg cgctcatccc gccgactcct gctcctttgt tccttccgtc acgattccac
    11041 tatacaggac gaccgtctct caaacgttac ggctggcgtt acgagaccga gatgcacacc
    11101 tggttttgga gagtcgctac ttcgaaaagc atcctcctgg taagtcccgc ggcccgtaag
    11161 ccaccgctaa gatcttcctg taacgaacgc tacccgaaga tagccagggg caaatacgag
    11221 gaaacgatcc agttcggctg gtctacgatc tcggagatct tcaggtcgac caccaggctc
    11281 gccagcacgt aggcctcgac gggctcgagc ccgtaggtcg cgcttatgtg ctcgaccatc
    11341 gcccttagag agtccttcgc ggcctgcatc agatcctcgt tcacgcccat cgtcgcgtac
    11401 cagcctgcct cgtcgaccga cggcgttagc ggtcccggag tctggaattg gggtgccggt
    11461 atgctcctgc ccttgatcag gtcgaagcgc aacgcggcgt acattggaca ctcgatacct
    11521 gtgacgcaga cctcgccgtc gccctgggcc gcgtgggcat cgccacagga gaagagcgcg
    11581 cctgaatgct gcacgggcag gtacagtacg gtgcccttcg tgatgtggcg cgtatccata
    11641 ttgccgccga acggtccagg cggcatgatg actggcttct cggggcctga tgggctaaca
    11701 cccatcgtcc cgaagaaagg ttctatgggg atggcgatgt cgtccttgaa cagcgtgtaa
    11761 tcgccgttgg tgagatcgaa gatcttcagg taggcgtcgg ggaagtcctc gggcaacagc
    11821 ccgaaacccg gtaagacggc ggtccagccc cagcctgcgg tatgcatatc cagtacctcg
    11881 accgccagca tgtcgcccgg ttcggcatcc gccatcgcga cggggccggc gagcgggtag
    11941 aggctgtccc agtcgaggct cgaaagggcg tccacggtgg agtccggccc tatctgcccg
    12001 tccgagacct cgcgggtgtg gtagacgacc gtgtctccag gctcgaccgt gagcaccggc
    12061 tcctgctcgt tgtcccagac gtagtggacc caatcttctg gaaagtaatg cgtcttcggc
    12121 atgccccgaa cctcctcgtc gtcccttgcc ctcgacatac tctctcagtt tgcggcatct
    12181 ctttccagtg gaatggctcg cgcgtcgccg atccggggcc tgtttctgcg cacttctgat
    12241 gtaggctgtt ctaagaaatt cggaagccaa cttgcaacat cgcgcagcac gcgccctggt
    12301 gggttacgta cccagccttc cactcactca agtgaataag cccggcacga catcccacta
    12361 gcgacagtaa ccttttacag gtcgcttgcc aacaccgccg gtccctattc accgtagttc
    12421 ggagttggtg ctacgatggt gtctctacat aagggacacc cttgaagcga gggagaacca
    12481 tgagacgcct gtacgtactg acagcctccg ccgtgatagc gacgcttctc atcgcgctta
    12541 cgatgagcac cgtaagcgca cagggggtga gtaagcgtca cggagcgata gccgccgtcc
    12601 cgtcgttggt aaggggcatc gacggttacg gcgccacgct gaagcaagcc aagagcgccg
    12661 cgatcgagga gtgtcgtaag gtcgcatcgc acaggcgcaa atacgctgag gactgccagg
    12721 gcgccgtctg ggtgcgtaac ggttggatag cgatcgcctt cgagcgtacc gttgaaaagc
    12781 cctatcgtga cctggcctgg ggctcgggct ggggtcacac gaaggacagc gccaaatacc
    12841 aggcccgaac agtttgccgg caatacgccg aggagaagtg cgaggtaaag agcacgtacg
    12901 agacgcccgt gagggacccg tccctggggt caaagggtgg tagctggtaa ctgttcccaa
    12961 ggtctctggg gccggggctc tgtcaccctc ggcccttcct ctttccccta ttcgccggag
    13021 tacgtggaac cctggatgat tgagcacccg acgatgcgcc aaaaagccgg cggtggtgag
    13081 catgactgac gatagaacgg gatacaaacg ctacctttca caaaggtatc tggcctccct
    13141 gcccgaacga accgcgcggg cgggagccgc actgacaggc gggttggtat acgagacggg
    13201 tgaggtgtta ctaccgctcg ctgttcgccg atccaggctc taccaggcgg tagtcggacg
    13261 gttgctgcgc atcatgatcg agctggttgg cggcgtggaa ggcgtctacc ctaccgaaga
    13321 gatgcctgtg cgagagctgc tggtacgcaa gtcggcgggc aatgtgatcg aactcagcag
    13381 ctttctggcg gtcggttggt caccggtctg gctcctggcg ggggcttccg acctggtcgg
    13441 gggtacgaag gtatacctgc gaacgctggt cacggagtta cgggatgcgg gagtactcgc
    13501 gccggacgcg gatgtggcat cattcgagga gctgttgact acactagagg gcacctccgg
    13561 tgtgctggcc gataccgttg acgtgccccc gttgaatatc tccagcgtgc gcacctcgtg
    13621 gcaacaacta cggctgcagg tagcggattt gcccgacgcc gagggtctag agaagatctt
    13681 ctccgaccta cagttggccg cacggcaaga ggatcggtcg gtactggaga tttcttccat
    13741 ggtcgcgctc ggcgcggtgc acgcgggcgt aaggctggga gatgctcaca tcttcgacta
    13801 ctatcgcaac gcgctgccga cgatagtcga agagggtctg gtctccttcc tgcgtcgagt
    13861 tagcacccct tacctgacgc gggcgcgttc gcactatgat cctgggtcga gcacttactc
    13921 agaacgcctc ctcaggcgtt gggcagaccg ccgttcatcc gtggcacgcc aaatgacgac
    13981 acaggatgca gcgtcgagca aggagaacgc tcctgaggat tattctactg gcgaggggca
    14041 agactagatg ccctcttcct cctccgcctc tagcaacgaa agcgtcatag gacaccaggg
    14101 gtggtgaata cgcaacgttg agtatccttg gggactcttg gcgaacctcg gaggaacagc
    14161 gttgcacacg ctacggtgaa tagagtacga tgcctgcaag atgcgcttag ggagagggcg
    14221 ccagaacgat gggggtgatt ccgagatggc taccgtagag gacctcgacg gagtgatcga
    14281 acgttgccag caagccatgc gcgagttcgg caagggaaac cccgaaccca tgaaggggat
    14341 gtattcccgt cgggaggacg tgaccatcaa caccgctatc gacccgcccg ctcgcgggtg
    14401 ggacgaggtc gccctgagga tggagcgctc cgcgtctgga ttcagggaag gcgagatcga
    14461 cgccttcgag accgtgacca gggtcgtcac cccggagctc gcctacgtcg tgtgggtgga
    14521 gcgtcacaga agcaagatcg gcgagaggga cgagatggtg gatttcccct tgagggtcac
    14581 gataatcttc cgtctcgagg atggatgaca tctggaaggt cgtgcaccgg cacacggata
    14641 cggtggtcag tccccggccg catgaatcga tgatcaaaga gtagagtatc ctgcctttga
    14701 gcaccaggcc ctgagtgtcc gccagcaggt gtcgctgtag tggcgtgggc gggcacaggc
    14761 gaaccactta cggtatctct tcgaggcggc tcgcgatgcg tgctgctctc tagactctct
    14821 cgaaggcctg ttcgaggtcg aagatcacat ccccggcgtg ctcgatacct accgagagcc
    14881 gcacgaggtt ctccctgacc ccgagcctct gacgccgcga gggcgagagc tcgcggtggg
    14941 aggaggtggc gggatggatg gcgagggtgt agatgtctcc tagagaagga gccttgacgc
    15001 agagctcgag cgcgttgagg taggcgaaag ccgcctcacg tccctcggcc gcaagctcga
    15061 aggagacgag gccgcccgtg tccgagagta cgcgccctgc ggtctcatgg tccggatgag
    15121 ccccgagcga aggatggttg accttcgcca ccttcggatg tgcggcgagg cttgacgcga
    15181 tctggcgcgc gttctcgcac tggcggccca tgcgcagggg cagcgtcttg aggccgcggt
    15241 gcgctagcca ggcctcgaaa ggaccgagca cgccaccgac aagcttgcgt agttgctcta
    15301 ccgccgccgc gtaaggcgga ccggcgacca ccgcgccggc cgtgaggtcc ccgtggcccg
    15361 agaggtactt ggtcgcgctg tggacgacta tgtccgcgcc gagctccagg ggccgctgga
    15421 gatacggtgt gccgaacgtg ttgtccacga tcagggctgc cccagcggcg cgggttatct
    15481 tcgcgatctc cggtacgtcg gcgacgcgta acagcgggtt ggagatggac tcgacgacaa
    15541 cagcgcgagg cttcagctct cccactttct tctccaccgc tcccaggtca tacgcgtcga
    15601 cgaagcgggt ctcgacgccg ctcgcgccga agatctggac gagcagcgtc accgtcgagc
    15661 cgtagagctg gtcggccgca aggaccgtgc ccccgccggt cagctccgcg gccgcgagcg
    15721 cggcgtgggt cgccgccatc cccgaggcga aggcgaaagc ccttacatcc ccttctgcct
    15781 cgggcgcctc caacgatacg agcgcctcct cgaaggcacg gacggtgggg ttgtcgtagc
    15841 gggcgtagct gtagccccgt tcatctccgc ctagcacgcg gtccgtggtc tcgatgttcg
    15901 ggtgcgagaa cgtggtcgaa gcgtagatag gcgtcgagat cgggtagaag atcccatccc
    15961 tgccttcctt tgcaccggcc ctgtgctctc cggagtgcac ggcgcgggtc tcgaaacgtc
    16021 tcgggtgctt catagtggct tatactacga cctggttgcc ccggtggcga ccacagcccc
    16081 gaagtcgagg ccgagatcgg agaggatcgt ctctgctgcg ttcctccccg gggccatcga
    16141 gacgcccggt ccacggtggc tccccgagcc gcacaggtag acgttctcta cgggggaacg
    16201 gtagtctccc atctcgggta cggggcgcat cgagcttcgc cgctctgtag cactcgacca
    16261 gcgcgcgcca ggtctcggcg tccttcttcg agtaccggcc gatattctcg acggttttct
    16321 ccaggtccct gctcacggcc aggaccctgc cgtcggaaat gtccctgagt ccatcgtctc
    16381 ctccccggag attcaactgt tgggctattg cacgccggga gaatatccgt gcatcttccg
    16441 gaagtctacc tacccatgac cagacgaaag atcgctcccc gacgtgttgt gcaagcggag
    16501 ggggatcctc tacttcgccg gggagcctgc ccgcattatc ggaccagtta cgttaagaga
    16561 gggtaacgtc cgtgtaccga ttctgttacg gcgcagaaaa tcgacctcgc gggcgacact
    16621 ctacagaggt aggttctatt cgcccaattg aataagtgcg agcctgagat cgtctgccat
    16681 tccgctgtgg ccgtgttttt cggactgggc gatccccttc tcgtaggcgg cgcgggcttc
    16741 atccttgcgc cccaatttgg agagcacgct gccgagacgc gcgtaagcgt tcccctcatc
    16801 gtcgacgtgg acggcaaggt aacgttccag caccgccgcc tcgtcttcgt cgcgcccggc
    16861 cttctcgtac tcgttggcca gggcaagcaa ccctgtcggg ttgtcagggt tgtcggcgag
    16921 catccgctcg aaatagtcta tcctgtcttc cacgaggcct cccttcgcgt ttaggctatg
    16981 ccagtataga agcacttcag cacgccgtct tcgacggctt gtcagccagt cagcatttca
    17041 gctaaaactt tagaagctga catgctgaga gccgcctggc gcagcctggc ggcgtgctga
    17101 caagcgaagc ctggcgaagc cgggcgtagc gtgctgaagt gctgacgggg tgggtacggc
    17161 accggggatg tggggtatca taagaaagat cgagatgttt gttgatcgct ttgcaggagc
    17221 gtttttgcgg ggtgcccgcg ataggtatcg ggcctcctcg cgcggcgctc gggccaaaga
    17281 gggtattgtc acgtgagggt gtgatccggt tggagagaga gacagagaac cagtttttcg
    17341 ggccgaacct cggctacgtg ttggaactct acgagaacta cagggaggac cccgattccg
    17401 tcgacggaca gatgcgggag ttcttcgaat cctggagccc gccgcgggta gggacgcccg
    17461 gcgccaacgg gcagcaggtc gcccctgagg tcgacgtgga gaaagccgta ggggcttcga
    17521 agcacgtaag gcacataagg gacttcgggc atcgggctgc gaggctcgac cctctcggga
    17581 gcgagccgcc tggcgacccg acactggatc cgaccttcta caggataacg aacgaggacc
    17641 tggagaccct gccctccctc atcatcggcg gacccatcgc cgagcgtaca tccaacgcgg
    17701 gggaggccgc cgaggagttg cgcaggatct actgcgacac cacggggtac gacttcgggc
    17761 acgtccacga ccccgaggag aggttctggc tgcgcgagtc cgtcgagtcc ggccgcttcg
    17821 aaaagcggat ggatggagag aatgcgatgc gtctcctcga gaggcttacg agggtagacg
    17881 ccttcgagaa attcctccac aagacgttcc tcggccagaa gcggttctcc gtcgaaggga
    17941 acgacatggt cgttccgatg ctcaacagga taatccgttc cgccgccgac gtcggcacgc
    18001 ccgtggtcac cctcggcatg gcgcacaggg ggaggctcaa cgtcctggcg cacgtgatgg
    18061 acaagcctta ctccaagata ttcggcgagt tccagcagcc cgagaagggc gagcagtcct
    18121 cggcggcgag cagccccggc gccgcctggg taggggacgt caagtaccat ctcggcatca
    18181 ggggtttcca gctcgctgac gaggaggccg acgaggaggt ctttatccac ctcgccccga
    18241 accctagcca cctcgagcac gtaaaccctg tggtggaggg tatggcccgc gcctcccagg
    18301 aagaactgga cgaggcaggc ccgccgcagc aggatgtaga cgcgtcactg cccatcctgc
    18361 tgcacgggga tgcggcgttt ccaggcgagg gggtctccgc cgagaccctg aacctgagcc
    18421 ggctgccggg ctacaggacc ggcggcacga tccacatcat caccaacaac cagctcgggt
    18481 tcaccacgga gcgtcaggat gcccgctcga ctacctacgc gagcgacctg gccaaagggt
    18541 tcgagatccc cgtggtccac gtcaacgccg acgatcccga ggcgtgcatg gcggtggcga
    18601 ggatggccca cgcctaccgc gagaagttca acaaggactt tatgatcgac ctcgtcggct
    18661 accgccgttt cgggcacaac gagggcgacg agcctgccta tacgcagccg cttatgtacg
    18721 agaccatccg cgatcacccc acggtgcgtg agatctgggc ccaagagctc gaacgccgcg
    18781 gtatcctgga cgagggcgag gcagaggcca tgttcgagga gatcatgtcc gccatggccg
    18841 aggtccaaaa gaaggccaca gacgaactca aggaagaaga cttcgccacg gacgagctgt
    18901 acacgccgct cgtcgggatc cccgagaccg ccgtcgcggc cgagaggctc tctgaactga
    18961 acgaggccct actcgaggtc cccga
//
LOCUS       DATMVF010000160         3880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_568416, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000160 DATMVF010000000
VERSION     DATMVF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568416"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1888)
                     /locus_tag="VN178_08100"
     CDS             complement(215..1888)
                     /locus_tag="VN178_08100"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HWS80972.1"
                     /translation="MIVICAIVAVLVAADFWLNSGKIHRGVEVGSVSLGGKTPAEARQ
                     IVRDQAMGPLKEFDFDGPAHFTRRAGEMGVSFNIDETVDKAYAVGREGNILERLSERL
                     RASFGGATIAPDIDYQAAKARGEVRELASQVNHPPTEANAKIYGSEVEVTESRDGYEL
                     DLAATMASVDNAIDDMSGETRLRGDVLDPGVTTAEAEVAAKKARGALSEELTLKAEGK
                     SWTLSPVDLGSVLEVTKQDGKIDVSLNRDLLDGRLTNVYNDLTVKPVEASYDFDADGD
                     IFVKPSHEGRSVEGEKLIDSIQGGLFEGKREYEVPVTVAKPQFTTTELEAKKPTELQG
                     TYRTNYTATTDQGQTRVENLKIASEAVSGTFVAPGQTFSMLDHVANLDYYNTHVIVDG
                     AETVDEGGGLCQVTSTLYNAALYAGMEITERTAHYSQLPYIRPGMDATVWYGGPGTAD
                     DLDMKFKNTTDGYVLLQEYVSNDGYIYANVYGVPDKVEVEMSSEPIFKVEDASKWVTY
                     YERTRNGKVVYSDQWETAYGALIDDEGKKIPTPQVPVAEIDGTYLGPAF"
     gene            complement(2213..2581)
                     /locus_tag="VN178_08105"
     CDS             complement(2213..2581)
                     /locus_tag="VN178_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80973.1"
                     /translation="MELRNKEELRSEILQSWLQSAWVYPFQGPDERTYLRLTPGGRLK
                     MRRRIGELERVLGVEGTELARQEQAGTLPAERDRLELAMMVQAYDTERRLIQSQGATL
                     GTPAVTLEEQGEEAPPDATP"
     gene            2663..3187
                     /locus_tag="VN178_08110"
     CDS             2663..3187
                     /locus_tag="VN178_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80974.1"
                     /translation="MGPMHEAPTPWRRLGETLEGSFTAHARGLLAPGFAILGKEGEEI
                     GRLQIHGAEGAELRAGDLEARIERSATSGYRMLTDDAVILTAKSLGAWNAPEIECLES
                     LYRGWLSLLRNTAEAGPAGERATIRISGGLTNRKYEAAFDAGDEGSLPVTLFLLYLTV
                     ALRRKAYLTGTRTS"
     gene            complement(3344..>3880)
                     /locus_tag="VN178_08115"
     CDS             complement(3344..>3880)
                     /locus_tag="VN178_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWS80975.1"
                     /translation="PARHKARGGALVVASVVILLLLLGGGGALASGLVGLPQGASVAD
                     TLSRMNPVETEPPAPPQGAQGDGTAGDTMDEPSGRSGSQNVASVTEAASSQARTPPRP
                     KKVPVPNVTAYYDYYAEDVLLNRGFDVRFVYDYQDGFAPRGVTWATDPAAGTLAPRGS
                     TVTVYATPKDLPLPRRLR"
BASE COUNT          661 a         1261 c         1182 g          776 t
ORIGIN      
        1 cagcatcgag gctcgtacgc aggaggctct ggtggaagcc ataggccaag cgctcgatgc
       61 tgtaagccgt agagacgctt tgggctggtt ttcacactgt ggttaccggc tgaccgatca
      121 atcattgtga aaagtgctct aactgacaag gcgcgaagcg ccgtgctgac aagcgacagc
      181 cgcaggctgg agcgtgctga cttgctgaag tgccctaaaa ggcgggcccg aggtaggttc
      241 cgtctatctc tgccacgggc acttggggag tcgggatctt tttgccttcg tcgtctatca
      301 gggcgccgta ggcggtctcc cattgatcgc tgtagacgac cttgccgttc ctcgttctct
      361 catagtacgt tacccacttg gaggcatcct cgactttgaa gatgggctcg gaggacatct
      421 ccacctcgac tttgtcgggt acaccgtata cgttggcgta gatgtagccg tcgttcgaga
      481 cgtactcctg caagagcacg tagccgtcag tggtgttctt gaacttcatg tccaggtcgt
      541 ccgccgttcc aggaccaccg taccagaccg tcgcgtccat gcccggcctg atgtaaggca
      601 actgcgagta gtgggcggtg cgctccgtta tctccatacc tgcgtagagg gcagcattgt
      661 agagcgtcga ggttacctgg cagagaccgc ctccctcgtc cacggtctcc gcgccgtcca
      721 cgattacgtg cgtgttgtag tagtcgaggt tggcgacatg gtcgagcatc gagaacgtct
      781 gacccggcgc cacgaacgta ccgcttacgg cctcagaggc gatcttgagg ttctccaccc
      841 gtgtctggcc ctggtccgtg gtcgccgtgt agttggtcct gtaggtgccc tgcagttcgg
      901 tcggcttctt ggcctcgagc tcggtcgtgg tgaattgcgg tttggccacc gtaacgggca
      961 cctcgtactc gcgcttgcct tcaaagaggc caccctgtat ggagtcgatc agcttctcgc
     1021 cttcgacgct ccgcccttcg tggctgggct tgacgaagat gtcgccgtca gcgtcgaagt
     1081 cgtagctggc ctcgacgggc ttgaccgtaa ggtcgttgta gacgttggtg agccggccgt
     1141 ccagaaggtc gcggttgagg cttacatcga tcttaccgtc ctgcttggtg acctcgagaa
     1201 ccgaccccag atccaccggt gagagcgtcc aactcttccc ctcggccttg agcgttaact
     1261 cctcggagag ggctcccctg gccttcttcg ccgccacctc tgcctcggcc gtcgtgaccc
     1321 cggggtcgag cacgtcgccc ctgaggcggg tctccccgct catgtcgtcg atggcgttgt
     1381 ccacgctcgc catggtggcc gcgaggtcca actcgtagcc gtcgcgggac tctgtgacct
     1441 ccacctccga gccgtagatc tttgcgttgg cctccgttgg gggatggttt acctgggagg
     1501 cgagctcacg cacctcgccc cgcgctttcg cagcctggta gtcgatatcg ggggctatag
     1561 tcgccccgcc gaaggaggcg cgcagacgct cggagagccg ctcgaggatg ttcccttctc
     1621 taccgacggc gtaggctttg tccacggtct catctatgtt gaagctaaca cccatctcgc
     1681 cggccctgcg ggtgaagtgc gctgggccgt cgaagtcgaa ctccttgaga ggacccatgg
     1741 cctggtccct gacgatctgg cgcgcctcgg cgggcgtctt gccgcccaaa gagacgctgc
     1801 cgacctccac gccgcggtgt atcttgcccg agttcagcca gaagtccgcg gccactaata
     1861 cggcgactat ggcgcagata acgatcacgg gtcccgcaag cccccttcgc cgtctaccgt
     1921 gcttgcccga gtcgaaagga ttcaagctgg acgagagacg accgtgagac gcgctctcat
     1981 attcgtttct cccgcgcctg tcccgctttc ttcggttctt gagactcaac tatccaccct
     2041 ccacgagcga cctttcatgc gcccgaatga cagattactg gagaggttcc ccctcaccct
     2101 cttggctggc gacacccagt cccggcccga acgagcgccg cccggaaagc ctaacacggc
     2161 aaggatgagt acgccaccta tttaccgatg ttcgtgagat cggactacca ggtcaaggag
     2221 tagcgtccgg tggggcctct tctccctgtt cctccagtgt caccgctggg gttccgaggg
     2281 tggcgccctg gctctggatc aagcgccgct cggtgtcgta agcctggacc atcatcgcga
     2341 gctcgagcct atcgcgctcg gcgggaaggg tacccgcctg ctcctgcctg gcaagctccg
     2401 taccctcgac gccaaggact ctctccagct ccccgatcct gcgacgcatc ttgagccgtc
     2461 ctcccggcgt caggcgcagg taggtgcgct cgtccggccc ctggaaggga tagacccacg
     2521 cgctctgcaa ccaggattga aggatctcgc tcctaagctc ctctttgttt ctaagctcca
     2581 tttagtcctc ttccgtctcc cgcgtaattt accgtcttcc atccggaaac tccaccgctt
     2641 tcccggcgta tggaagggta tcatgggccc catgcacgaa gcccccacgc cctggaggag
     2701 gctcggggag accctggaag gctccttcac agcccacgcg cgcggactcc tcgcccctgg
     2761 gttcgcaatc ctcggcaagg agggcgagga gatcggacgc ctgcagattc acggggccga
     2821 gggagccgaa ctcagagccg gggacctgga ggcgaggatc gaacgctccg ccacctcagg
     2881 ctacaggatg cttactgacg acgctgtgat tctcacggca aaatcgctcg gagcctggaa
     2941 tgcgcccgag atagaatgcc tggagagcct ctacagagga tggctgtccc tcctgcgcaa
     3001 cacggccgag gccggacccg ctggagaaag ggcgacgata cgcatctccg gaggcctcac
     3061 gaaccgaaaa tacgaggccg ccttcgacgc cggcgacgaa ggctcgctac ccgtcaccct
     3121 cttcctcctc taccttaccg tcgcactcag gcgcaaggct taccttacgg gtacgagaac
     3181 gagctgaaag cgcgggctcg tccagcgggg ttggttctac agcgcccctc gaaacgatag
     3241 tggacaaaga gctgaaatgc tgaactgctg acaagcgaag gctaaaagcc tgagcgtgct
     3301 gacaagcgcg cgtctcggaa ggcttggcct tccgagacgc gctttagcgg agccgcctgg
     3361 gcagcgggag gtccttgggc gttgcgtaga cggttaccgt ggagccccgc ggggccagcg
     3421 tccccgcggc cgggtccgtg gcccacgtca cgccgcgcgg tgcgaaaccg tcctggtaat
     3481 cgtagacgaa cctcacatcg aagcctctgt tgaggaggac atcctcagcg tagtagtcgt
     3541 agtaggccgt tacgttcggg acggggacct tcttcggcct gggcggcgtc ctcgcctggg
     3601 aggaagctgc ctccgtcaca gaggcgacgt tctggctacc ggaccggccc gagggctcgt
     3661 ccatcgtgtc gcctgcggtt ccgtccccct gcgcgccttg tggtggcgcc gggggttccg
     3721 tctccacagg gttcatccgg ctcaaggtgt cagccacgct ggcaccctgc ggtaggccga
     3781 cgagccccga ggccagcgcc cctccgccgc cgaggaggag cagcagaatc accacagagg
     3841 ctacgaccag cgcgccacca cgagccttgt ggcgcgccgg
//
LOCUS       DATMVF010000161         7835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_485789, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000161 DATMVF010000000
VERSION     DATMVF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485789"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VN178_08120"
     CDS             <1..1308
                     /locus_tag="VN178_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HWS80976.1"
                     /translation="FGALLEATAGFGTPVAITASLMAGLGFEALYAAALALLANTAPV
                     AFGGFGIPILTGASVAGLDTLELSQMVGRQTPLLALIIPAMLVTVMSGFRRMLEVWPV
                     IAVSGIAFAGTQFVVSNLLGPELADLLAALITVVAVVGLLSFWRPSSEWHFENEPSAE
                     ERESYETPPLGRTLYAWSPFIIIVALFLLVQVPPIKDAVSGVQTAVNFPSAELDPTTG
                     LPVVPWPGLDGQVEQRPPVVPEAAPYSATYATNWLSAAGTIILIADVIALAVLRVGPG
                     RALRIYGRTLKQLWSAVLTIASILGLAYLLNYSGVTYTLGLAFAATGVLFPFFASFIG
                     WLGVALTGSDTSSNALFANLQKVTAQQIGVSPNLTVGANSSGGVLGKMISPQNLAVGT
                     SATGLQGKEGDLLRLVLKWSVALTLVMAIWVTIQAYVLPFLVP"
     gene            complement(1281..1613)
                     /locus_tag="VN178_08125"
     CDS             complement(1281..1613)
                     /locus_tag="VN178_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HWS80977.1"
                     /translation="MHLYYGAVDLLRLFALSVGGSVIFAGIVLGARVARGAGGRARAA
                     RQIGEHAALGLDFFVGATLLNLAINPTGTVLATTAIIIVVRKLLTFSLGLPQREAGAA
                     YGTRNGNT"
     gene            complement(1623..1997)
                     /locus_tag="VN178_08130"
     CDS             complement(1623..1997)
                     /locus_tag="VN178_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HWS80978.1"
                     /translation="MEEYLVTAVGYLRLIVEAIGAAVIGFGVLATTYRYVLTLLGLSD
                     YTNNQVRLYLGRYLALGLEFQLGADILGTAVAPTLDDVILLGAIAAIRTVLNFFLSQE
                     LERERQQEAAARETSEQRPLPG"
     gene            complement(2199..2657)
                     /locus_tag="VN178_08135"
     CDS             complement(2199..2657)
                     /locus_tag="VN178_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80979.1"
                     /translation="MHLANYLGYLHKAELHLAEAYRKVGEGHAEEADVYHACNTLAEQ
                     CEVHAGKLGPFVERYGEAAPAEPDRLYHELFDETRSGGLGLLRDMQDLYLMANACDIS
                     WTIIGQAAQGLSDRDLLEVVDSCEGQTATQIKWLKTRMKQAAPQALLVAS"
     gene            complement(2662..5109)
                     /locus_tag="VN178_08140"
     CDS             complement(2662..5109)
                     /locus_tag="VN178_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HWS80980.1"
                     /translation="MTDRIANPWGKRTPFGRDGEWPVRVDQFLEAGVSEEDVDRWVQT
                     ASILHSNGDALDIALREGRIVGVRGRSVDRVNKGRLDPKDLFGWQANNAEDRLTRPLV
                     RTDGELVEAGWDEAFERIVGRSKALLEEKGPLAFGFYTTGQLFLEEYYTLGVIGKAGL
                     GTPHMDGNTRLCTATAGQALKETFGTDGQPGSYSDIDHADAILLFGHNLAETQAVTWM
                     RVLDRLAGPHPPNLVVVDPRPTPVAREATVHLPIKSGTNVALLNAFLHQILENGLYDE
                     EYVEAHTIGFDELRRTVAGCTPGWAAEICGVEEGRIREAARILGESERLLSTALQGVY
                     QSHQATAAACQINNIHLLRGMIGRPGCGVLQFNGQPTAQNTRETGADGDLPAFRNWDN
                     KEHVQELADLWNVERMKIPHWAPPTHAMQIFRYAEQGSINLLWISCTNPAVSLPELQR
                     IRDILKREDLFLVVQDIFLTETARLADVVLPAATWGEKTGTYTNTDRTVHLSEAAVRP
                     PGEAKHDLDIFLEYARRMDFRNRDGGPLIEWEHPEEAFEAWKECSRGRPCDYTGITYE
                     KLRGGSGIQWPCNEEHPDGTERLYTDGDFNTRTEECETYGQDLLTGASNTESEHRAMK
                     PDGRAFLKAANYTPPHEEPGEEYPFRLNTGRTIYHFHTRTKTARAPQLQNAAPEMWVE
                     IHPSDAEGLGVGEGDVVRVESPRGSLEAKARISGIREGVVFAPFHYGYFDDSDGASPA
                     GRPRAANELTITEWDPVSKQPLFKMAAVKVTKVADSGGEPAPAPTTTASAPVSGGSIP
                     ETVGGEEAEVVETVGGE"
     gene            5367..6098
                     /locus_tag="VN178_08145"
     CDS             5367..6098
                     /locus_tag="VN178_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWS80981.1"
                     /translation="MALFITCFNDTLFPETGRATVEVLERLGVEVDFPYAQTCCGQMH
                     FNTGYQREAIPLVRRFVEIFGGYEAVVAPSGSCVSMVRELYPMAADLAGNGRLAERVE
                     ELGPKVFELSEFLVRELGVTDVGAYYPHRVTYHPTCHSLRMLEVGDAPLELLSNVRGI
                     DLVELSEAEECCGFGGTFAVKNADTSAAMLSAKIRRVLDTEAEVCTAGDNSCLMHIGG
                     GLGRLRTGVKTVHLAQILASKAEEG"
     gene            6098..7561
                     /locus_tag="VN178_08150"
     CDS             6098..7561
                     /locus_tag="VN178_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HWS80982.1"
                     /translation="METKKTDAFSPGPTPTFEESAKESLSDSQLRRNLGKATRTIRHK
                     RAVAVEEMPDWEGLREAGRALKERVVRHLDEYLLQLEESVTRAGGEVHWARDAEEANR
                     IISGIVKDKGAEEVVKVKSIATDETKLNEHLKKEGIEAFETDLAELIIQFAGETSSHI
                     LVPAIHKNRAEIRELFIQELGARNLSDRPSDLTEAARLYLREKFLAAGVGISGANFAV
                     AETGTVCVVESEGNGRMCTTLPPVLVTLMGIEKVIPAWQDFEVFMQLLPRSSTAERMN
                     PYTSFWTGVSGEAGDGPREFHLVLMDNGRTDVLADETGRQALNCIRCSACLNVCPVYE
                     RTGGHAYNSVYPGPIGAILTPQLVGIGDPGPDSLPYASSLCGACYQVCPVKINIPEVL
                     VHLRGKVVRHKQYMDGPRSKLDPENVAMQTVARVFSDPELYEKAQRLARIGQWPFARG
                     GSIGRLPGRLAGWTAVRDLKPVPRQTFREWWRNRGSS"
     gene            7561..>7835
                     /locus_tag="VN178_08155"
     CDS             7561..>7835
                     /locus_tag="VN178_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80983.1"
                     /translation="MATAKETILWRIRHATRDVPEAERPEDVSVRREYRKEDGAGREE
                     IVARFAERTAEYEADVRRVGHDKLPAAIEEALKRRGVRHLVIPPYLP"
BASE COUNT         1299 a         2610 c         2522 g         1404 t
ORIGIN      
        1 ttcggggcgc tcctggaggc gacggccggc ttcgggacgc cggtcgccat cacggcctcc
       61 cttatggccg ggctcggctt cgaagccctc tacgcggcgg cgttggcgct gcttgcgaac
      121 accgctccgg tcgcgttcgg cgggttcggc atacccatcc tgaccggggc cagcgtggcc
      181 ggactagaca ctctggagct ctctcagatg gtgggacgcc agacgccgct gttggcgctc
      241 atcatccccg ccatgctcgt cacggtgatg tcgggctttc ggcggatgct ggaggtgtgg
      301 ccggtgatcg cggtcagcgg catcgccttc gcggggacac agttcgtggt ctccaacctc
      361 ctgggtcccg agctcgccga cctgttggcg gccctgatca cggtcgtcgc agtggtcggg
      421 ttgctctcgt tctggcgccc ttcgagcgag tggcacttcg agaacgagcc ctcggccgaa
      481 gagcgcgaga gctacgagac cccacccctc gggcgcacgc tctacgcgtg gtcgccgttc
      541 atcatcatcg tcgcgttgtt tttgttggtg caggtgcccc cgatcaagga cgccgtaagc
      601 ggcgtgcaga ccgccgtaaa cttcccctcg gcggagttgg acccgacgac agggttgccc
      661 gtcgtgccgt ggcccgggct cgacgggcag gtcgaacaac gacccccggt cgtacctgag
      721 gcggcccctt actccgccac ctacgccacc aactggctct cggcggcggg cacgatcatc
      781 ctgatcgccg acgtcatcgc cctagctgtc ttgagggtcg ggcccggacg ggcattgagg
      841 atctacggcc ggacgctcaa acaactctgg tccgccgtcc tcacgatagc ctcgatcctc
      901 gggctcgcct acctcctgaa ctactcgggc gtgacctaca ccttaggcct cgcgttcgcc
      961 gcgaccggcg tgctgttccc gttcttcgcc tcgtttatcg ggtggctggg ggtggcgctt
     1021 acgggatccg atacctcttc gaacgccctg ttcgccaatt tgcagaaggt cacggcccag
     1081 cagatcgggg tcagcccgaa ccttacggtc ggagccaact cctcgggcgg cgtgctgggg
     1141 aagatgatca gcccacagaa cctcgccgtc ggcacgagcg ccacaggatt gcagggcaag
     1201 gagggcgacc tcctgcgcct ggtcctgaaa tggtccgtcg ccctcaccct cgtgatggcg
     1261 atctgggtta cgatccaggc ctacgtgtta ccgttcctgg tcccgtaggc ggctcctgcc
     1321 tccctctgcg gcaggcccag cgagaaggtg agcagcttgc gcacgacgat gattatggcc
     1381 gtcgtcgcca ggaccgtccc cgtggggttg atggccaggt tcagcagcgt ggccccgacg
     1441 aagaaatcca gtccgagggc ggcgtgctcc ccgatctggc gcgcggccct ggctcttccc
     1501 ccagccccgc gggcgaccct ggccccgagc acgatcccgg cgaagatcac cgagcccccg
     1561 accgagagcg cgaacaacct gaggaggtcg accgcgccgt aatagaggtg caccccgacg
     1621 ccctagccgg gcaacggacg ctgctcggag gtctcccgcg cggcggcctc ctgctggcgc
     1681 tcgcgctcca gctcctgcga gaggaagaag ttgaggaccg tacggatcgc tgctatcgcg
     1741 ccgagcagga tgacgtcgtc gagggtcggg gcgaccgccg tccccaggat gtcggcgccg
     1801 agctgaaact cgagcccgag ggcgaggtag cgcccgaggt acaatcgcac ctggttgttg
     1861 gtgtaatcac tgaggccgag cagcgtcagg acgtagcggt aggtcgtagc cagaacgccg
     1921 aaccctatca ccgcagcgcc tatcgcctcg acgatcagcc gcaggtaccc gaccgccgtt
     1981 accaagtact cttccatcgc gggtccgtct cctcgatctc gaactaccgt aaccgccatg
     2041 cgccgcgtac gcaggccggc gccccgcaaa ccctaacact acgtcgctcg cgcgtttgcc
     2101 cgcgtcgggc tcatcccttg gccgaccaca ggagcacgag accccggcgg cccggttgac
     2161 gagccgctcg tgaaggccgt ggcgctcgcc ctccgccgtc aagacgccac cagcagggcc
     2221 tgcggagcag cctgcttcat gcgggtcttg agccacttta tctgcgtggc cgtctgcccc
     2281 tcgcaggagt cgacgacctc cagaagatcg cggtcggaca agccctgggc cgcctgtccg
     2341 atgatggtcc agcttatgtc gcaggcgttc gccatcaggt agagatcctg catgtcacgc
     2401 agaagtccga gaccgccgga tctcgtctcg tcgaagagct cgtggtagag gcggtcgggt
     2461 tccgccggcg ccgcttcacc gtagcgctcc acaaacggtc cgagcttacc ggcatgcacc
     2521 tcgcactgct cggcaagagt attgcaagcg tgatagacgt cggcctcctc cgcgtggccc
     2581 tcacccacct tgcgataggc ctcggcgagg tggagctccg ccttgtgcag gtaccccagg
     2641 tagttggcca ggtgcatgat cctactcccc tcccacggtc tcgaccacct ctgcctcctc
     2701 cccgcccacg gtctcgggga tcgacccgcc cgatacgggt gctgaggccg tcgtcgtcgg
     2761 ggccggggcg ggctcaccgc ccgagtccgc gaccttcgta acctttacgg cggccatctt
     2821 gaagagcggc tgcttggaga cagggtccca ctcggtaatg gtcagctcgt tggccgcccg
     2881 gggacgacct gccggcgagg ctccgtccga atcgtcgaag tagccgtagt ggaacggcgc
     2941 gaagacaacg ccctcgcgta tcccgctgat gcgggccttc gcctcgaggc ttccgcgcgg
     3001 ggactcgacg cggactacgt cgccctcccc taccccgagc ccttcggcat cggagggatg
     3061 gatctccacc cacatctcag gggctgcgtt ctggagttga ggtgctctgg cggtcttggt
     3121 tcgggtgtgg aaatgataga tcgtccgccc cgtattcagg cggaaagggt actcttcgcc
     3181 cggctcttca tgtggcggcg tgtagttcgc ggccttgagg aaggcccgac cgtctggctt
     3241 catcgcccgg tgttcggact cggtgttgga cgcgcccgtg agcaggtctt gcccgtaagt
     3301 ctcacactct tcggttctgg tgttgaagtc cccgtcggtg tagagacgct cggtaccatc
     3361 tggatgctcc tcgttacaag gccactggat gccagaacca ccgcgtaact tctcataggt
     3421 gatgcccgtg tagtcgcagg gacgccctct gctgcactcc ttccaagcct cgaaggcctc
     3481 ctcgggatgc tcccactcga taagcggccc tccatctctg ttgcggaaat ccatgcgcct
     3541 cgcgtactcc aggaagatgt cgaggtcgtg cttcgcctcc ccagggggcc gtacggcggc
     3601 ctccgagagg tggaccgtgc ggtcggtgtt cgtgtacgtc ccggtcttct cgccccaggt
     3661 cgcggccggc aagacgacgt cggcgagccg ggccgtctcc gtcaagaaga tgtcctgcac
     3721 gacgaggaag aggtcttccc tcttcaggat gtcccttatc ctttgaagct cggggaggga
     3781 gacggcgggg ttcgtgcaac ttatccagag gaggtttata gagccttgct cggcgtagcg
     3841 gaagatctgc atggcgtgcg tgggcggcgc ccagtgcggg atcttcatcc gctccacgtt
     3901 ccacagatcc gcaagttctt gaacgtgctc tttgttatcc cagttcctga aggccgggag
     3961 gtcgccgtcg gcgcccgtct cgcgggtgtt ctgcgccgtg ggctggccgt tgaactgcag
     4021 gacgccgcag ccgggacggc cgatcatgcc ccgcagcaaa tggatgttgt tgatctggca
     4081 ggccgccgcc gtcgcctggt gggactggta gacgccctgc agcgcggtgc tgaggagccg
     4141 ctccgactct ccgaggatgc gggcggcctc cctgatcctg ccttcctcta cgccacagat
     4201 ctccgcagcc cacccagggg tgcaccctgc aacggtgcgc cttagttcgt cgaaaccaat
     4261 cgtgtgggcc tcgacgtact cctcatcgta gagtccgttc tccaggatct ggtgcagaaa
     4321 cgcattgagg agcgcgacgt tggtgccgct ctttatgggg aggtggacgg tcgcctctct
     4381 ggcaacgggc gtggggcgcg ggtctacgac gactagattt ggcgggtgcg gcccggcgag
     4441 cctgtcgagg acgcgcatcc aggttaccgc ctgcgtctcg gcgaggttgt gcccgaatag
     4501 aagtatggca tcggcgtggt ctatgtcgga gtaggaaccc ggctgtccgt cggtgccgaa
     4561 cgtttccttg agcgcctggc ccgcggtcgc cgtgcaaagg cgggtgttgc cgtccatgtg
     4621 cggcgtcccg agaccggcct taccgatcac accaagggta tagtactcct cgaggaagag
     4681 ctggcccgta gtgtagaacc cgaatgccag cggtcccttc tcctcgagca aagccttcga
     4741 ccggccgacg atacgctcga aggcctcatc ccagcccgcc tcgacgagct cgccatctgt
     4801 acggacgagc ggccgcgtca ggcggtcctc tgcgttgttg gcctgccagc cgaagaggtc
     4861 tttggggtcg aggcgtcctt tgttgacgcg atccacgctc ctgccccgca ccccgactat
     4921 gcggccttct ctgagggcga tgtcgagggc gtcgccgttg gagtgcagga tggaggccgt
     4981 ctggacccag cggtcaacgt cctcttcgga gacgccggct tccaggaact ggtccaccct
     5041 cactggccac tccccgtccc tgccaaacgg cgtgcgtttg ccccaggggt tggctatacg
     5101 gtcggtcatg gtctctcctt acctgtgcgg gctcgtgaaa gcgaccgtgc ttctatggac
     5161 tctatctcgg cgcgccgtga acgcgcgtac tcgaccgccg aagacgtatc catgacacca
     5221 gtatctccaa atgagtcccg atgtaaccct gctcccggtc cgacgcgagc gtggcacccg
     5281 ccagggcaac gagggtcgct gggccctgga atgggctatc atggtgctct tggaggcgaa
     5341 ggagacgggg gaccgtagca gtgcgcgtgg cgctctttat aacctgcttc aacgacacgc
     5401 tctttcccga gacgggacgg gctaccgtcg aggttctcga acgtctgggg gtggaggtag
     5461 actttccgta cgcgcagacg tgctgcggcc agatgcactt caacaccggc taccagcgcg
     5521 aggccatacc gctggtgaga cgctttgtgg agatcttcgg cggttatgag gccgtcgtcg
     5581 cgccctccgg ctcctgcgtg agcatggtgc gtgagctcta cccgatggcc gcggacctcg
     5641 cagggaacgg gcgcctcgcc gaacgggtcg aggaactcgg cccgaaagtc ttcgagctct
     5701 ccgagttcct ggtcagagag ctgggcgtca ccgacgtcgg cgcctactac ccgcaccggg
     5761 tgacctacca cccgacctgc cactccttga ggatgctcga ggtcggagat gctccgctgg
     5821 aactgttgag caatgtgcga ggcatcgacc tggtggagct ctccgaggcc gaggagtgct
     5881 gcgggttcgg cgggaccttc gcggtcaaga acgcggacac ctccgccgcc atgctctcgg
     5941 ccaagatccg gcgcgtcctc gacaccgagg ccgaggtctg caccgcgggg gacaactcgt
     6001 gcctgatgca catcggcggc ggactcggcc gccttcgtac gggggtaaag accgtgcatc
     6061 tcgcccagat actcgccagc aaagccgagg agggctagtg gagacgaaga agacagacgc
     6121 attctcccca ggcccgaccc cgacgttcga ggagtcggcg aaagagagcc tctccgactc
     6181 gcagctcagg cgcaacctcg gcaaggccac ccggaccatc cgccacaagc gcgccgtcgc
     6241 cgtcgaagag atgcccgact gggaagggct cagggaggcc ggccgcgccc tcaaggaacg
     6301 cgtcgtgcgc cacctcgacg agtacctgct gcagctcgaa gagtccgtga cgcgcgcagg
     6361 gggcgaagtc cactgggcgc gcgacgccga ggaagcgaac cgcataatct ccgggatcgt
     6421 caaggacaag ggcgcagaag aggtcgtcaa agtcaagtcc atagcgacag acgagaccaa
     6481 gctcaacgag cacctaaaga aggagggtat agaggcgttc gagacggatc tcgcggagct
     6541 catcatccag ttcgcgggag agacctcttc gcacatcctg gtgccggcga tccacaagaa
     6601 ccgcgctgag atcagggagc tctttatcca agagctcggc gcacgaaatc tgtccgacag
     6661 accttccgac ctgacggagg cggcccgcct gtacctgcgc gagaagttcc tggctgccgg
     6721 ggtcggtatc tcgggggcga acttcgccgt ggccgagacg gggaccgtct gcgtcgtcga
     6781 atccgagggc aacggcagga tgtgcacgac gttgccgccg gtccttgtta cgctcatggg
     6841 tatagagaag gtcatcccgg cctggcaaga cttcgaggtc tttatgcaac tcctcccccg
     6901 ctcctctacc gcagagcgga tgaaccctta cacctcgttc tggaccgggg tgtcgggcga
     6961 agccggcgac ggaccccggg agttccatct cgtcctcatg gacaacgggc gtaccgacgt
     7021 gctggccgac gagacagggc gccaggccct gaactgcatc cgttgctcgg cttgcctcaa
     7081 cgtctgcccc gtctacgagc gaaccggcgg ccacgcctac aactccgtct acccgggccc
     7141 gataggcgcg atcctgacgc cccagctcgt cgggatcggc gaccctggtc ccgactcgct
     7201 gccctacgcc tcgtcgctct gcggggcctg ctaccaggtc tgtccggtca agatcaacat
     7261 cccggaggtc ctcgtccacc tccgcggcaa agtcgtccgt cacaagcagt acatggacgg
     7321 cccgcgctcg aagctcgacc ccgagaacgt cgcgatgcag acggtcgcaa gagtgttctc
     7381 cgaccccgag ctctacgaga aggcccagag attggcgagg atcggccagt ggccgttcgc
     7441 ccgcggaggc tctatcggtc gcctaccagg caggctcgcc ggctggaccg ccgtacgcga
     7501 cctcaagccc gtcccacggc agaccttccg agagtggtgg cggaacagag ggtcctccta
     7561 gtggcgactg cgaaagagac gatcctgtgg cgtatacgac acgccacccg tgacgtgcca
     7621 gaggcggagc gtcccgaaga cgtatccgtc agacgcgaat accgcaagga agacggcgcg
     7681 gggcgcgaag agatcgtcgc gcgcttcgcc gagcgcacgg cggagtacga ggcggatgtc
     7741 cgtcgcgtgg gccacgataa gctccccgca gccatagaag aggccctgaa gcggcggggc
     7801 gtgagacacc tcgtcatccc cccttacctc ccgag
//
LOCUS       DATMVF010000162         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_348194, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000162 DATMVF010000000
VERSION     DATMVF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348194"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VN178_08160"
     CDS             <1..508
                     /locus_tag="VN178_08160"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWS80984.1"
                     /translation="FDFSTGAGVESGFVALVRPDGSAQKVAGNIAFGNGMAITEDGST
                     LIVAESWARRLSAFDIAADGGLSNRRIWADTGDGPPDGICMDAEEAVWYADVPNKRCV
                     RVREGGEVLQIVELDRGGFACMLGGPDGRTLFMLTAAWTGMENVDDSARTGQLLVAEA
                     PAPGVGRP"
     gene            833..2068
                     /locus_tag="VN178_08165"
     CDS             833..2068
                     /locus_tag="VN178_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS80985.1"
                     /translation="MRKRVSATAESGRTRLRGFLLVLGIVLLAANLRPALTGVAPLIG
                     QIRADTGMSNGEAGLLTTLPLLAFGLLSPVAPRVARRFGMERVLLAGLLVLVAGILLR
                     WAGAVAALFLGTVILGAAIAVANVLLPSLVKREFPEQAGLMTSVYSTSLGISAAFAAG
                     VSVPLAQLAGIGWRGALAVWAIPALLAGVAWLPQLGRSDRPADPSARGSIGVRDLWRS
                     PLAWQVTLFMGLQSLAYYVMLTWLPEILQAEGMGAARAGWMLGLSQAVAILTMFLAPM
                     IAGRTPSQRGVVVVAVGTSGVGALGLLVAGSTASTLWVVLLGLGQGASFSLALTFFAL
                     RSPDPGHAAALSGMAQSVGYLLAAGGPFLFGVLRDVTGAWKVPLALLLTITVCLLIAG
                     IGAARDAHVGTSIPEEAGG"
BASE COUNT          320 a          688 c          785 g          437 t
ORIGIN      
        1 cttcgacttc tcgaccggcg cgggagtgga atccgggttc gtcgcgctgg tgcgtcctga
       61 cggctcggcg cagaaggtgg ccggcaacat cgcgttcggc aacggcatgg ccatcaccga
      121 agacggctcg acgttgatcg tcgccgagtc ctgggcccgg cggcttagcg ccttcgacat
      181 cgccgcggac ggtggcctgt cgaatcgccg gatctgggcc gatacgggtg atggtccgcc
      241 cgatgggatc tgcatggacg ccgaggaggc ggtctggtac gccgacgttc cgaacaagcg
      301 ttgcgtgcgg gtacgcgaag gaggcgaggt gctgcagata gtcgagctcg atcgtggcgg
      361 cttcgcctgt atgctcggcg gaccggacgg gcggacgctg ttcatgctca cggccgcgtg
      421 gaccggcatg gagaacgtag atgactcggc gcgaaccggt cagctcctcg ttgcggaggc
      481 gcccgcaccg ggcgtcggtc ggccgtagga gggcagcagg ttcggttgcg gcgtcccgcc
      541 acacggggga gcgcggcgtg tgcgggaagt tgccgagaac gtcgccaggt gctgctgtag
      601 caggtccgct tcgcctacgt ggatgctatt taggcatcaa cctgcaggat atccttcccg
      661 ggtgcactta tgacctacga gaggctcccg gacttcgttt agagtcgcat gagggtatcc
      721 tacgcctacc tgcccctcat gcacctggca cagttccggg gccgtggcgg gtgctcgatc
      781 ggggagatcg acggtccatt ctcctccacg acgagagaca gggaggcgca gtatgaggaa
      841 gagggtttcc gccacggcag aatccggccg cacacgcctc cgtggctttc tcctcgtatt
      901 ggggatagtg ctcttggcgg ccaacctgcg cccggcactc accggcgtgg cgcccttgat
      961 cggccagatc cgggctgaca ccggcatgtc taacggggaa gcggggcttc tcacgacgtt
     1021 gccgctcctg gctttcggcc tgctctcgcc ggtcgcgccg cgcgtagccc ggcgattcgg
     1081 catggaacgc gtgctgcttg cgggtctgct cgtcctcgtg gccggcatcc tgttgcgctg
     1141 ggcgggggct gtggcggctc tattcttggg tacggtcatc ctgggagcgg cgatcgctgt
     1201 agctaacgtg ctcttgccga gcctcgtcaa gcgagagttc cccgaacaag ccggcctcat
     1261 gacgagcgtg tactccactt cgctaggcat cagtgcagcg ttcgccgccg gtgtaagcgt
     1321 acccctggca cagctggccg gtatcgggtg gcgaggagcc ctggcggtgt gggccatacc
     1381 cgcccttctc gccggtgtcg cctggctccc ccaactcggc cgtagcgatc gtcctgcgga
     1441 tccgtccgcc cgaggctcca ttggggttag agacctgtgg cgttcgcccc tggcctggca
     1501 ggtgacgctg ttcatgggct tgcagtcgct ggcatactac gtgatgctga cgtggctacc
     1561 ggagatcctg caggcggagg ggatgggggc ggccagggcg ggatggatgc tcgggctctc
     1621 tcaggccgtg gcgatcttga cgatgttcct ggcgcccatg atcgccggga ggacgccctc
     1681 tcagcgaggc gtggtcgtgg tggccgtggg cacgtcaggt gtcggcgctc taggccttct
     1741 tgttgccggt tccaccgcca gcaccctgtg ggtcgtgctg ctaggtctcg gtcagggggc
     1801 gtccttcagc ctggcgctca ccttcttcgc gcttcgatct ccagacccag gacacgccgc
     1861 tgcgctctcg ggtatggcgc agtcggtcgg ttacctgctc gccgcgggtg gacctttcct
     1921 cttcggcgtc ctgcgcgatg tgacgggtgc atggaaggtg ccgctggcac tgctgctgac
     1981 gattacggta tgcctgctga tcgcgggaat tggcgccgcc cgcgacgccc acgtcggtac
     2041 atccatcccg gaagaagcgg ggggataagt gctcgtccga gaagactcca cattcaagag
     2101 cgtagattgg tctggaccgt tcgatgccgg ttcgcaacga ggaagtgggc gcaggaaagc
     2161 tatacactcc ggcctatgag cgagagcacc gagcgtgagg tccccctggc cttgatggtc
     2221 caggcgccag
//
LOCUS       DATMVF010000163         5393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_660765, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000163 DATMVF010000000
VERSION     DATMVF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660765"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VN178_08170"
     CDS             complement(<1..926)
                     /locus_tag="VN178_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80986.1"
                     /translation="MNYGDLIRDAFTITLHNRYLWFFGFFAGGTGSNFVGNVPPGSGN
                     FNFDNFRRPGSDVSGLTAQVGQGLSETALILGIVVVVLIIALFFIVLAIISQGALADS
                     VAAIDRGQGRRFGSTFTSGMGNFWRVLGYYIVFFLVALGLLVVIAIPTALLIGGTFAA
                     TQVTGVRVSVAVVVGILAVLLLIVVFIPLSIIGQYALRDIVVRRERVLGSVGSGYGHF
                     RHNIGRSLLIWLIHLGLSIGIGIAFILLLLIVGLVLFIPTIALAVAGSSTGAIIAGVI
                     AGLILLPLLLVAAGAIGTFSHAYWTLAYLRLTA"
     gene            complement(923..2323)
                     /locus_tag="VN178_08175"
     CDS             complement(923..2323)
                     /locus_tag="VN178_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWS80987.1"
                     /translation="MRFERALPGTNEVFFPQGTLRPLYEPVFDELERTGPRKWEEKLR
                     EAHGLLLDEQHAFGIEEGDKTHPTDWIPRIVSAPDWERLEAGLTQRLRAINVFLSRLE
                     SGKEEVVPREVVESSILYDTGWKDRFGPVPVRQIAFDVVAVEVGRGWEYLVIEENAKM
                     PVGLAAMTRRRRMSGEVFFPGSMASLPVLPLDGWLERLGGALRAASPRGPDATLAVVS
                     SGPDDQFYLDHHIYAKEMDAVLAETKDLEVDGNGYLIYRPTGRRIDVIYERVDEDALY
                     AEVPGLLEAHIEGKVHVLFAPNSEIIDDKGVGAFVPEMVRRYLGEEPLIENARTWSLA
                     VEEDRRYVMDHFAELVVKSRGGYGGKEVMIGPEESRESLQKFRRRVEENPVEYVAQET
                     IDFSTHVLCETEAADFLLRDSYADYRVLVLSPDPEDPYVVEAVPGSLSRVAAPGKHVV
                     NISSGGKMKDTWVLGS"
     gene            2526..2750
                     /locus_tag="VN178_08180"
     CDS             2526..2750
                     /locus_tag="VN178_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80988.1"
                     /translation="MVYSALYGGGANITAGALGVGFGVLGYFLGARKLATVTVFLCAT
                     AILFGLAASQGFVPGIAPSDRTFPNLSSLF"
     gene            2810..3550
                     /locus_tag="VN178_08185"
     CDS             2810..3550
                     /locus_tag="VN178_08185"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HWS80989.1"
                     /translation="MERIRLTPPGEKRARGGRARLYVRWMPHLGAEAVALYELLRILP
                     DLGNESPEIEELAELLRSTPEGVEASLEALVEARFVVRRDGWLEVAEHPPSAETGERP
                     REDVLPDRDVEVIRAEPEGVSADDYFRFMGTLPAPHILEFLNGYCGRDGLTPDAVREA
                     LKIASERDARRVGYVRSILERWVERGVKTPEDVERFEQDRKLRLVEQSGAVRGGALKI
                     REGVSGGPDRQSAARRREGYEWLFGETE"
     gene            3480..4346
                     /gene="zapE"
                     /locus_tag="VN178_08190"
     CDS             3480..4346
                     /gene="zapE"
                     /locus_tag="VN178_08190"
                     /inference="COORDINATES: protein motif:HMM:NF005992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HWS80990.1"
                     /translation="MDRIDSLLPDAGKAMSGSSGRRSKRQAEVLRLDDKICPYCGREL
                     QAEWDEFPPALQKKYGKPGEWYYHPCTPECEKKNERREWELMRREARVATLRDRSGLS
                     KRMKGYSLDNFDPSFSRNTQKAFAKVMDYIMGWKANQEGGRGLYFCGPVGVGKTHLAV
                     AVMNEIITKKRVPSLFVTVPELLDNLRGAYNDPGRDIDEWMDSVKNADLLLLDDLGAE
                     RANEWVRERIFVIVNHRYREELPTIFTSNTGPKDLAAQLGERTASRIIAMCDWISLEG
                     EDYREAEVRRGL"
     gene            4343..5014
                     /locus_tag="VN178_08195"
     CDS             4343..5014
                     /locus_tag="VN178_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80991.1"
                     /translation="MREEFMIVRQGKQYVLYSGLLDEAHSLGLTGIDTDLIQVPDESN
                     RNVAIVKATAEMEDGRRFSGIGDASPENVGRNIAPHLIRMAETRAKARALRDAVNVGA
                     TALEELSDGDDVPPAAQSSSSRGRPTPIRGGRQNSSQGSSQSATAEQDTPNGQPEKPA
                     SSKRGGAGKARKSQVDLLKTLAVEWRGENGVERLENRLGKPLQDMTRAEADEWIDRLT
                     PEGRE"
     gene            5107..>5393
                     /locus_tag="VN178_08200"
     CDS             5107..>5393
                     /locus_tag="VN178_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80992.1"
                     /translation="MATRKYLLAPLLLVLILLWPSCAELELLTGGSRGGPGPPPSGSL
                     SVSFIDVGQGDAVLVQAGGESYLIDAGRPEEGPNVVDFLRSRSVDALDGIVV"
BASE COUNT         1038 a         1671 c         1783 g          901 t
ORIGIN      
        1 gctgtcaggc gcaggtaggc gagcgtccag taggcgtggg agaaggtgcc tatggcgcca
       61 gcggccacga gcaggagggg gagcaggatc agacccgcta tcacgccggc gatgatggcc
      121 cccgtggatg acccggcgac ggccagcgcg atggtcggga tgaacaggac gaggccgacg
      181 atcaggagca gcaggataaa ggcgatcccg atcccgatag agagcccgag atggatgagc
      241 cagatgagca ggctccttcc gatgttgtgg cggaagtgac cgtagccgct ccccacggag
      301 ccgaggacgc gctccctacg caccacgatg tcccgcaggg cgtactggcc gattatggag
      361 agggggataa agacgacgat gagcaagagg acggccagga tccccaccac caccgcgacg
      421 gagacccgca cccccgtgac ctgggtggcc gcgaacgtgc ccccgatcag gagcgccgtg
      481 gggatggcta tcaccacgag gagcccgagt gcgaccagga agaacacgat gtagtagccg
      541 aggactcgcc agaagttacc catcccggac gtgaaggtgg acccgaaccg gcgtccctgg
      601 cccctgtcta tggcggctac gctatcggcc agcgctccct gggagattat cgccaggacg
      661 atgaagaaca gcgcgatgat tagcaccacc acgactatac cgaggatcag ggccgtctcg
      721 ctcagtcctt gcccgacctg ggccgtgagc ccggagacat ccgagcccgg gcgccggaag
      781 ttatcgaagt tgaaattgcc actaccggga gggacgttgc ccacgaagtt ggagcccgtg
      841 ccgcccgcga agaagccgaa gaaccacagg tagcgattgt ggagcgtgat cgtgaatgcg
      901 tcccgaatga ggtctccgta gttcatgatc cgagcaccca ggtatctttc atcttgcctc
      961 cactcgagat gttgaccacg tgcttgccag gagcggcgac ccggctgagc gatccaggga
     1021 cggcctcgac gacgtacggg tcttcggggt cgggagagag gacgagcacg cggtagtccg
     1081 cataggagtc gcggaggagg aaatccgccg cctctgtctc gcagaggacg tgggtggaga
     1141 agtctatcgt ctcctgggcc acatactcca cagggttctc ttcgacccgc ctgcggaact
     1201 tctgaagcga ctctctcgac tcttctggtc cgatcatgac ttccttgccg ccgtagccac
     1261 ccctgctctt cacgaccagt tcagcgaagt ggtccatgac gtagcgcctg tcctcctcta
     1321 ccgccagcga ccaggtcctg gcgttctcta tgaggggctc ctctcccagg taacggcgca
     1381 ccatctcggg gacgaaggcg ccgacgccct tatcgtctat tatctctgaa ttgggggcga
     1441 agagcacgtg tactttgccc tcgatgtggg cctcgagcag gccgggcacc tcggcataga
     1501 gcgcatcctc gtcgacgcgc tcgtagatca catctatcct gcgtcctgtg ggcctgtaga
     1561 tgaggtatcc attaccgtcc acttccagat ccttcgtttc ggcgaggacg gcgtccatct
     1621 ccttggcgta gatgtggtgg tcgaggtaga actggtcgtc aggaccactg ctcacgacgg
     1681 ccagcgtcgc atccggtcct ctgggtgagg cggcccgcaa agcgccgccg aggcgctcga
     1741 gccagccgtc gagaggcagg acggggagac tggccatgga accggggaag aagacctccc
     1801 cgctcatcct gcgcctgcgg gtcatcgcgg cgaggccgac cggcatcttg gcgttctctt
     1861 cgatgaccag gtactcccag cctcgtccga cctctacggc gacgacgtcg aatgctatct
     1921 ggcgcacggg cacgggaccg aaacgatcct tccagcccgt gtcgtagagg atgctactct
     1981 ccacgacctc cctcggtacg acctcctcct tgcccgattc gaggcggctc aggaacacgt
     2041 tgatggcgcg caagcgctgc gtcaggccgg cctcgagacg ttcccagtcc ggggccgaaa
     2101 cgatgcgggg gatccaatcc gtaggatgtg tcttgtcgcc ctcctctatg ccgaacgcgt
     2161 gctgctcgtc gagcaggagc ccgtgcgcct cgcgcagctt ttcttcccac ttcctggggc
     2221 ccgtgcgttc caactcgtcg aagaccggct cgtagagggg gcgtagcgtc ccttggggaa
     2281 agaagacctc gttcgtgcct gggagtgcgc gctcgaatct cacaggagca ggattatact
     2341 agcccccggg cagggtacgc cctgtcgtgc cggtaggcaa cggcccatgg gagtgaattc
     2401 tgggcgaaaa ctcggacaca cggatacccg aagagcaagc gggcaacgat ccaataccag
     2461 agggacgcgc gaagctgctc ggtggctgga tctcctgcgc cgtcggagca ctcctcgcag
     2521 caggcatggt ctactccgcg ctctatggcg ggggcgcgaa catcaccgcc ggagcgctcg
     2581 gcgtgggctt cggggtcctc ggctacttcc tcggcgccag aaaactcgcg accgtgaccg
     2641 tcttcctctg cgccactgcc atcctcttcg ggctggccgc cagccagggc ttcgtccccg
     2701 gcatagcgcc ctcggatcgc accttcccca acctctcgtc cctgttctga gtcttcctta
     2761 cgaatcagac gtagagagca tggccctcgg ccgctatact ggccgcagga tggagagaat
     2821 tcgtctcaca cctccgggag agaagagggc gcggggaggc cgcgcccgtc tctacgtccg
     2881 ctggatgcca cacctggggg ccgaggccgt cgcgctctac gagttgctcc gtattctgcc
     2941 cgaccttggg aacgaatctc ccgagatcga agagcttgca gagctgttgc gctccacacc
     3001 ggagggcgtc gaggcgtctc tggaggccct ggtcgaagcc cgcttcgtcg tccgcaggga
     3061 cggctggctc gaggtcgccg agcacccgcc ttcggcagaa acgggcgagc ggccgcgcga
     3121 agacgtgctt cccgaccgcg acgtcgaggt gataagggcc gaacccgaag gggtctcggc
     3181 cgacgattac ttccgtttta tggggacgct gccggcgccg cacatcctgg agttcttgaa
     3241 cggttactgc gggcgggacg ggcttacgcc agatgcggta agggaggctc tgaagatagc
     3301 cagcgagcgc gacgcgcgca gggtcggcta cgtgcggagc atcctggagc gatgggtcga
     3361 gcggggcgtg aagacgcctg aggacgtgga gcgcttcgag caggaccgca agctcaggct
     3421 cgtcgagcag agcggggccg tgaggggtgg tgccttgaag atcagagagg gggtgagtgg
     3481 tggaccggat agacagtctg ctgccagacg ccgggaaggc tatgagtggc tcttcgggga
     3541 gacggagtaa gcgccaggcc gaggtcctga ggctcgacga taagatctgc ccctactgcg
     3601 ggcgcgagct tcaggccgag tgggacgagt tcccgcccgc cctccagaag aagtacggca
     3661 agccgggcga gtggtactac catccctgca ccccggagtg cgagaagaag aacgagcggc
     3721 gcgagtggga gttgatgcgc cgcgaggcgc gggtcgcgac actaagggac cgtagcggcc
     3781 tttccaagcg catgaagggc tactcgctcg acaacttcga tccctcattt agcaggaaca
     3841 cccagaaggc tttcgccaag gtcatggact acatcatggg ctggaaagcc aatcaggagg
     3901 gcgggcgggg tctttacttc tgcggcccgg tgggcgttgg aaagacgcat cttgctgtgg
     3961 ccgtcatgaa cgagatcatc accaaaaagc gggtgccttc gctcttcgtc acggtgcccg
     4021 aacttctgga caatctgcgc ggagcttata acgaccctgg cagggatatc gacgagtgga
     4081 tggactccgt aaagaacgcg gatctcttgc tgctcgacga cctcggcgcc gagagggcta
     4141 acgagtgggt gcgcgagcgc atcttcgtta tcgtcaacca ccgctacaga gaggagctgc
     4201 cgaccatctt cacctccaac acgggaccca aggacctggc ggcccagctc ggggagagga
     4261 cggcgagcag gatcatcgcg atgtgcgatt ggatctccct ggaaggcgaa gactaccgcg
     4321 aagccgaagt aaggcggggc ctgtgagaga agagttcatg atcgtccgcc agggaaagca
     4381 atacgtcctg tattcgggcc ttctcgacga agcccatagc ctgggactga cggggataga
     4441 cacggacctg atccaggtcc ccgacgagtc aaacaggaac gtcgccatag tgaaggctac
     4501 ggcagagatg gaggacggcc gtcgcttctc gggtatcggg gacgccagcc ccgagaacgt
     4561 ggggcgcaac atcgccccgc acctgatccg gatggccgag accagggcca aagcccgcgc
     4621 cctgcgcgac gccgtgaacg tcggcgcaac cgccctcgaa gagctctccg acggcgacga
     4681 cgtccctccg gccgcccaaa gctcgtcatc gcgcggcagg cccactccca tccgtggggg
     4741 gagacagaac tcctctcaag gctcttccca gagtgccacc gccgaacaag acaccccgaa
     4801 tggccagccg gagaagccgg cctcctcgaa gcggggtggg gccggcaagg cgcgcaagag
     4861 ccaggtcgac ctcctgaaga cgctggccgt cgagtggcgg ggcgagaacg gcgtcgagcg
     4921 cctggagaac cgcctgggca agccgctcca ggacatgacc cgcgccgagg ccgacgagtg
     4981 gatcgaccgc cttaccccgg aagggcgcga gtaacataag gaccctcgaa gtaggcagcc
     5041 caaagaggtg ttagggacca ggcgattagg cctaaggttt ctcggaacca tccgataaca
     5101 tacctgatgg ccacacgtaa gtatctcctc gctccactgt tgctggtcct gatcctgctt
     5161 tggccgtctt gcgcggagct ggagttactc accggcggtt cccggggcgg tcccggccca
     5221 ccgccttctg gctctctatc cgtgagcttt atcgacgtgg gccaggggga cgcggtgctc
     5281 gtgcaggccg gtggcgagag ctatctcatc gatgcgggac ggcctgagga ggggccgaac
     5341 gtggtggatt tcttgcgcag caggagcgtc gacgccctcg acggcatcgt ggt
//
LOCUS       DATMVF010000164        10946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_568668, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000164 DATMVF010000000
VERSION     DATMVF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568668"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VN178_08205"
     CDS             <1..562
                     /locus_tag="VN178_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWS80993.1"
                     /translation="DVRAVERLLEQGAGVDARDESGRTALIAAAYGNRLDVAGLLVDA
                     GADVNAKDETQQSAYLISTSEVGDDPRLLELTLRNGANVNSLDSYNGTGLIRASERGY
                     GRVVERLLRTDIEVNHVNNLGWTALLEAIILGDGGRSHTEVVRLLVGAGANVNLADGE
                     GVTPLEHARRRGYVKIVRILEAAGAR"
     gene            complement(584..1489)
                     /locus_tag="VN178_08210"
     CDS             complement(584..1489)
                     /locus_tag="VN178_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWS80994.1"
                     /translation="MAGPVRDQWAQWLLERRFGGDPGQREKHLGYLIPWRDRILENAA
                     VQRGETFLDVGAGDGLIAFGALDLVGEDGRIILSDISQDLLDHARSLAEKMDVADRCE
                     FLLAPADDLSALGCASVDVVTTRSVLIYVEGKRRAFEEFFRVLKPGGRISLFEPINRF
                     NTVAWSAESGLYMGYDVTPVKDLAVRVQAIYERIQPRDTDPMLNFDERDLLTHAQEVG
                     FEEIHLEYRADITQGNHLFSGEGPGWELLLTSSGNPKIPTLEEAMNEALKPEEIERFT
                     AHLRPLVERNESKGMSAVAYLWAIK"
     gene            complement(1501..2457)
                     /locus_tag="VN178_08215"
     CDS             complement(1501..2457)
                     /locus_tag="VN178_08215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWS80995.1"
                     /translation="MPEKVLVTREIPEAGLRLLRDFDVAVLAEGPPAREELLEAARGA
                     AGILSTVTESVDAELMDAAGEDLKVVANMAVGYDNIDLDAAGERDVVVTNTPEVLNET
                     TADTAFMLLLAAARRLGEAERLLRSGGWDAWGPMQLTGPDVWGKKLGVLGFGRIGQSV
                     ARRARGFDMDVLYHDKYRNERAENETGVRYVGLEELLRTCDFISVHTPLTPETRHLIG
                     RGELDLMRPTAVLVNSSRGPVVDEAALADALEEGIIFAAGLDVYEEEPEVHPKLLGLE
                     NVVLAPHIGSASIETRDRMAELAAENLVAVLSGEEPKTPVSG"
     gene            complement(2479..5430)
                     /locus_tag="VN178_08220"
     CDS             complement(2479..5430)
                     /locus_tag="VN178_08220"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWS80996.1"
                     /translation="MANDPFGARTTLESGSADVEFYSLRTLDENVNGDVFSLPFSIRV
                     MLESLLRNAGGKFVSKEDVEALAGWPGSVGGELAYLPARVVMQDFTGVPAVVDLAAMR
                     SAMSNVGGDPGRINPLVPTDLVIDHSVQVDAFGSPAALQFNAEREFERNRERYEFLKW
                     GQQAFDNFSVVPPATGIIHQVNLEYLAKGVIVKDGVAMPDTLVGTDSHTTMINGLGVL
                     GWGVGGIEAEAVTLGQPYYMLVPQVIGFKLTGALREGVTATDLVLTVTQMLRSHGVVG
                     KFVEFYGEGLSKLSLPDRATIANMSPEFGATCTFFPVDTETLRYLRGTGRDEELVELV
                     ESYSKEQGLWRTDETPEPRFSETLDLDLGSVEASLAGPRRPQDRVALDDMQPSFRQAL
                     AEMVDPDHPLLGNGREDAYDRADAESFPASDIPNPDIPVAQGGPDEAEGKEGGAVSTG
                     ESHTADDTESFPASDTPAGAAEATDEGGGDPTSGGGPENGQAEAGPTGTITVTVDGEE
                     LSLGHGSAVIAAITSCTNTSNPSVMMGAGLLARKAVEKGLSVAPHVKTSLAPGSKVVT
                     EYLQTSGLLDPLEKLRFDVVGYGCTTCIGNSGPLAEEISAAVEENDLVVAAVLSGNRN
                     FEGRINPDVRANYLASPPLVVAYALAGTVDIDLSKDPIGEDEDGNPVYLQDIWPSQEE
                     VAREIENALDPEIYKEQYADVYTGNEQWNEVEVPSGDLYEWDPDSTYIQEPSFFKDMG
                     PEPEDLKDIEGARVLVKVGDSVTTDHISPAGAIPSKMPAGQYLIQKGVDPRDFNSYGS
                     RRGNHEVMVRGTFGNIRLRNQLVDKEGGYTIHLPDGEETTIYEASLKYEEENVPLVVL
                     AGKEYGSGSSRDWAAKGSFLLGVRAVIAESFERIHRSNLIGMGVLPLQFSEGESAESL
                     GLTGHESFDIAGLAGLEPGKELTAKATSDEGEEKEFQVKARVDSPVEVEYLRNGGILQ
                     TVLRQMLEEED"
     gene            complement(5562..7178)
                     /locus_tag="VN178_08225"
     CDS             complement(5562..7178)
                     /locus_tag="VN178_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HWS80997.1"
                     /translation="MNPPSGDRIGSPRRWSVALLGAAVAVFVGVLYLGTLAPTVLPYG
                     VPDTLDSPMLQTEVSVLGIGHPTGYPTYMMLTHLFTYLPLGDSAYRVNLASAVYGVAA
                     VLLVYLAGLRLARRAVAAAAGALAFGLSGAFWSQAVIAEVYSLEALLVGLVIFVLLVW
                     RDRRVGRYLLLSAFLVGLSLTHHLTSVLLVPGALAFIFLTDRRVFSRTGLMLKAVGLF
                     LLGLLPLLYLPIRALMHAPLNEADPSTPWRFLLLVTGGSFLAESSEKGRQCNPSSLAL
                     ADASTKLGLFGDHLLGQFSFALMVVGALAAVYLLFTDRAAAILLGVLFFGCLGQAGVY
                     LQLGIEDFYVFLIPVFLVFGLCISLGLGALLRIVEGLAISSSARGALVVALSVLMLFV
                     PLLGVREAYVEHDRSEDYAARRTIEAVASKVEKGATVLHHRSPLWYMALVEGSRHDLT
                     LIDPFCTSWDRHADVVWPDRINAAEAAERYGTDDTTGVETARRAARSGPVYLLANGRA
                     PLDTFREAGFDVVPVDEDDLLYKLVPEERR"
     gene            7242..7781
                     /locus_tag="VN178_08230"
     CDS             7242..7781
                     /locus_tag="VN178_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS80998.1"
                     /translation="MAKRGYTRDTIRGGASDWDVSRPLSSYARTLATLLVHPVRFFEL
                     LPRIPDMRAPTLFLIFSGLPAAISWLLFWGFYPALVALVLPLPLSFFLAGLYHLGALG
                     GRHGYVVTWRVLAYPLGFYLPLAAVPVLGLLGAAYSGLVLMGIGLAEVQEIGRLWAGL
                     FCTAVVVVFGAAYYFSSLA"
     gene            complement(7801..8283)
                     /locus_tag="VN178_08235"
     CDS             complement(7801..8283)
                     /locus_tag="VN178_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS80999.1"
                     /translation="MDAKSKIKTREATAEDVARIHILVRELAEKVGDEPPTEEAVRAR
                     LEELLDEPRARVLVAENEAGIVGGVSFWIKPDLAHGDTVVEVPMLVVGEDHRRTGVGK
                     LLMEEVRNVASDNGASQIELVATRANVTAREFYRSLGFVEADVVSLEYVGSQEDPPEE
                     "
     gene            8384..9553
                     /locus_tag="VN178_08240"
     CDS             8384..9553
                     /locus_tag="VN178_08240"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HWS81000.1"
                     /translation="MGCRTVVIVLDGLGAGNAPDAADFGDEGANTLSNTAKATGGIEA
                     PNLQTLGLGNIGEIEGISPTSSPAASHGLMVEMSAAKATLAGHWEMMGLVLEDPLPTY
                     PEGFPEEILRRFENETGRGVIGNRPASGTRIIEELGPEQEKTGNWILYTSADSVFQIA
                     AHTRVIPLDELYEASKKAHKMLIEEGEIMVERVIARPFHGESGAYVRENENRHDYGIT
                     PPSETYLDRIKVAGHEVVAVGKIRDIFDGRGITEHLPAPPDDAAKVGAILQALGRVES
                     GLIFANLVDFDAKYGHRRDPEGMAENISRFDARLPELLDAIGPNDLLIITADHGNDPT
                     FNGTDHTRERVPLLVVGGGEPLDLGVREGFSDVGASLAAWLGVEKGRLPGVDLLK"
     gene            complement(9698..10033)
                     /locus_tag="VN178_08245"
     CDS             complement(9698..10033)
                     /locus_tag="VN178_08245"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HWS81001.1"
                     /translation="MQKGRKEIKSWDEVPSFASEKEEQAWWDEHDISEELLETFEPRS
                     ERSNLPPPRERTITRPRKNPVSVRMENDLVNRLKVLAAVKGVGYQTLMRQFVADRVYE
                     EEKREGIIG"
     gene            10705..>10946
                     /locus_tag="VN178_08250"
     CDS             10705..>10946
                     /locus_tag="VN178_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81002.1"
                     /translation="MSNTTQKIECFDERLPELLDALTAADLLIISADHGNDPTFKGTE
                     PSPLPFVDPGLRPPAPFPTSCSTPGEEVAAIPSIPKS"
BASE COUNT         1954 a         3571 c         3386 g         2035 t
ORIGIN      
        1 tgatgtgcgg gctgtggagc gtctcctcga acagggcgcc ggcgtggatg ctcgcgacga
       61 gagcggtagg acggccctga tcgccgccgc gtacgggaac cgtctggatg tggcggggct
      121 gctcgtcgat gccggcgcgg acgtcaacgc caaagacgaa actcagcaga gcgcgtacct
      181 gatctccacc agcgaagtag gcgacgatcc tcgtctactg gagctgacct tgcgcaacgg
      241 cgcgaacgtc aacagcctcg acagctacaa cggtacgggc ttgatccgcg catccgagcg
      301 aggctacggc cgggtcgtcg agcggttgct ccggaccgac atagaggtga accacgtcaa
      361 taaccttgga tggaccgccc tgctggaggc catcatcctg ggagacggcg gccggagcca
      421 cactgaggtc gtccggctac tcgttggggc aggagccaat gtgaacctgg ccgacggcga
      481 gggcgtaacg ccgctggaac acgcccgccg gcgaggttac gtaaagatcg tcaggatcct
      541 ggaggccgcc ggagcgcgtt aacgatttta tcgaaagctt ctcctacttt atggcccaga
      601 ggtaggcgac ggcagacatc cctttgctct cgttgcgctc caccagcggg cggaggtggg
      661 cggtgaagcg ttcgatctcc tccggcttca gcgcctcgtt catcgcctct tccagcgtgg
      721 ggatcttggg gttccctgag ctcgtgagaa ggagctccca gcccggacct tcgccggaga
      781 acaggtgatt accctgcgtg atgtctgccc ggtattcgag gtggatctcc tcgaacccca
      841 cctcctgtgc gtgggtgagc aagtcacgct catcgaagtt caacatcgga tccgtgtctc
      901 tcggctggat acgctcgtag atggcctgaa cccgcacggc gagatccttt accggggtca
      961 cgtcataacc catgtaaagg cccgactctg ccgaccaggc aaccgtgttg aagcgattga
     1021 tcggctcgaa aagggagatt cggcccccgg gcttcaggac gcgaaagaac tcttcgaaag
     1081 cgcggcgttt gccttcaacg tagatcagaa cggagcgcgt cgtcacgacg tccactgaag
     1141 cgcaacccag cgcggagagg tcgtccgccg gggcgagcag gaactcgcaa cggtccgcga
     1201 cgtccatctt ctccgccagg ctccgggcat ggtccagcag gtcttgcgag atgtctgaga
     1261 ggatgatccg gccgtcttcc ccgacgaggt cgagcgcgcc gaaggcgatg agaccatccc
     1321 cagcccccac gtcgagaaag gtctcgccac gttgcactgc ggcgttctct agtatccggt
     1381 cccgccaggg gatcaggtac cccagatgtt tttcccgctg cccggggtcg ccaccgaagc
     1441 gccgctccaa cagccactgg gcccattggt cacgtaccgg ccccgccatc gccggactct
     1501 ctacccactg accggtgtct tcggctcctc gccggagagg acagcgacga ggttctcggc
     1561 ggccagctct gccattctgt cgcgggtctc gatggaggcg ctgccgatgt ggggggcgag
     1621 gacgacgttc tcgagcccca gaagcttggg atgcacctcg ggttcctcct cgtacacgtc
     1681 gaggccggcg gcgaagatga tcccctcctc cagtgcatcg gcgagggccg cttcgtccac
     1741 caccggacca cgcgaagagt tgactaggac ggccgtgggt cgcatgaggt ccagctctcc
     1801 ccggcctatg aggtgtctgg tctcgggcgt gagaggagtg tggacggaga tgaagtcgca
     1861 cgtgcggagc agttcttcga gcccgacgta ccgcacgccg gtctcgttct ccgcacgttc
     1921 gttgcgatac ttgtcgtgat acaggacgtc catgtcgaac ccccgggcgc ggcgggctac
     1981 ggattgtccg atcctaccga accccaagac cccgagcttc ttgccccaga cgtcgggacc
     2041 cgtgagctgc atcggtcccc aggcgtccca cccgccggag cgcaggagac gctccgcctc
     2101 gccgagtctc ctggccgccg ccagcaagag catgaaggcg gtatcggctg tcgtctcgtt
     2161 caggacctcg ggggtgttgg tcacgaccac gtcccgctcc ccggcggcgt cgaggtctat
     2221 gttgtcgtaa ccgacggcca tgtttgcgac gaccttgagg tcctcgccgg ccgcgtccat
     2281 caactccgcg tccacactct ctgtgacggt ggagaggatg cctgcggccc cgcgtgcggc
     2341 ttcgaggagt tcttcgcgcg cgggcggtcc ctctgcgagc accgcgacgt cgaagtcccg
     2401 aaggagacga agaccggctt cgggtatctc acgggttacg agtaccttct ccggcatgcg
     2461 accctcccta tcctggctct agtcttcctc ttcgagcatc tggcgcaaca cggtctgcag
     2521 gatgccgccg ttacggaggt actcgacctc caccggggag tccacgcgcg ccttgacctg
     2581 gaactccttc tcttcgcctt cgtcgctagt ggccttcgcg gtcaactcct tacccggctc
     2641 gaggccggcg agaccggcga tgtcgaaaga ctcgtgaccc gtaaggccga gagattctgc
     2701 gctctcgccc tcggagaact gcaagggcag cacgcccatg ccgatcaggt tcgagcggtg
     2761 gatgcgctcg aagctctccg cgataacggc cctgacgccg agcaggaacg agcccttggc
     2821 ggcccagtcg cgcgaggaac ccgacccgta ctccttgccg gccaggacga cgagcggcac
     2881 gttctcctct tcgtacttca ggctcgcttc gtagatcgtg gtctcttcac catcgggaag
     2941 atggatggtg tagccaccct ccttgtccac gagctggttg cggagcctga tgttgccgaa
     3001 cgtgccacgc accatgacct cgtggttacc gcgcctggag ccgtaggagt tgaagtcgcg
     3061 cgggtcaacg cctttctgga tcaggtactg accagccggc atcttggaag ggatggcgcc
     3121 tgcgggcgag atgtggtcgg tggtcacgga gtccccgacc ttcaccagga cgcgcgcgcc
     3181 ctcgatgtcc ttcaggtctt cgggctcggg ccccatgtcc ttgaagaacg agggctcctg
     3241 gatgtaggtc gagtcggggt cccactcgta aaggtccccg ctgggcacct ccacttcgtt
     3301 ccactgctcg ttgccggtgt aaacgtcggc gtactgctct ttgtagatct cggggtcgag
     3361 ggcgttctct atctcgcggg ctacctcctc ctgggagggc cagatgtcct gcagatacac
     3421 ggggttgccg tcctcgtcct cgcctatagg gtctttggac aggtctatgt ccaccgtgcc
     3481 cgccagggcg taggcgacga cgagcggtgg ggaggccagg tagttggcgc ggacgtcggg
     3541 gttgatgcgg ccttcgaagt tgcggttgcc cgagagtacg gccgcgacta cgaggtcgtt
     3601 ctcttctacg gcggcggaga tctcctcggc cagcggaccg gagttcccga tgcaggtcgt
     3661 gcacccgtag ccgaccacgt cgaacctcag cttttctaga gggtcgagga gcccggaggt
     3721 ctgcaggtac tccgtgacga ccttggatcc cggcgcgagg ctggtcttga cgtgcggggc
     3781 gacggagagc cccttctcca cagccttcct ggccaggaga ccggccccca tcatcacgga
     3841 agggttggag gtattggtgc agctcgttat ggcggcgatc acggcggagc cgtgcccgag
     3901 agagagctcc tccccgtcca ccgtgacggt gatggtgccc gttggcccgg cctcagcctg
     3961 gccgttctcg ggcccgccgc cggatgtcgg gtctccccca ccctcgtcgg tggcctcggc
     4021 tgcgcccgcg ggggtatcgc tcgcggggaa agattcggtg tcgtccgcgg tgtggctctc
     4081 acccgtagag acggcgcccc cctccttgcc ctcggcctcg tcgggcccac cttgggcgac
     4141 cggtatgtca gggttcggga tatcgctcgc ggggaaggat tccgcgtcgg cacggtcgta
     4201 ggcgtcctcg cggccgttcc ccaggagcgg gtggtcggga tccaccatct cggccagcgc
     4261 ctgcctgaag gagggctgca tatcgtcgag ggcgaccctg tcctgcgggc ggcgcggccc
     4321 ggccagactc gcctcgaccg accccagatc cagatccagc gtctccgaga agcgcggctc
     4381 tggtgtctca tccgtgcgcc agaggccctg ctccttgctg tacgattcga ccagctccac
     4441 gagttcttcg tcacggcccg tgcccctgag gtagcggagc gtctccgtgt ctacggggaa
     4501 gaacgtgcac gtcgccccga actcggggga catgttcgcg atggtggccc tgtcaggtag
     4561 cgagagcttg ctcaagccct cgccgtagaa ttccacgaac ttgcctacta cgccgtggga
     4621 tcgcagcatc tgggtgaccg tgaggacgag gtcggtggcc gtgacgccct ctcgcagggc
     4681 gccggtgagc ttgaagccta taacctgggg gacgagcatg tagtagggct ggccgagggt
     4741 gacggcctcg gcctcgatgc cccctacgcc ccagccgagg acgccgaggc cgttgatcat
     4801 ggtggtgtgg ctgtcggtcc cgacgagcgt gtctggcatc gccactccat ccttgacgat
     4861 cacacccttc gccaaatact ccaggttgac ctggtggatg atccccgtag ccggaggcac
     4921 gaccgagaag ttgtcgaagg cctgctggcc ccacttcaag aactcgtagc gctcccggtt
     4981 gcgctcgaac tcgcgctcgg cgttgaactg gagggcggcc ggagatccga aagcgtcgac
     5041 ctgcaccgag tggtcgataa ccaggtcggt cggcacgagc gggttgatcc tgcccggatc
     5101 cccccccacg ttcgacatcg cgctccgcat cgccgcgagg tcgaccaccg caggcacccc
     5161 ggtaaagtcc tgcatgacga ccctggcagg caggtacgct agctccccgc cgaccgaccc
     5221 cggccagccg gccagcgcct cgacgtcctc tttggagacg aacttcccac cggcgttgcg
     5281 caagagggac tccagcatca ccctgatgga gaacggtagt gagaacacgt ctccgttcac
     5341 gttctcatcc agggttctca ggctatagaa ttctacgtca gcagaacctg actccagcgt
     5401 cgtgcgcgca ccgaagggat cgttcgccat gtccgcaacc tctctctgaa tctcgtatgc
     5461 gtgacctcct tcgattatag atgatcggac cgcggttacc ggtacacata cgactcatag
     5521 tcaggaagag ataggttact cggctccacg aatccctcag gctacctgcg ttcctctggc
     5581 acgagcttgt agaggagatc gtcttcatcg accggcacca cgtcgaaacc cgcctcgcgg
     5641 aaagtgtcca atggggcacg gccgttggca agcaggtaga cgggtcccga ccttgcggcc
     5701 ctgcgcgcgg tctccacacc ggtggtgtcg tcggtgccgt agcgctcggc agcctcggcc
     5761 gcgttgatcc ggtcgggcca gacgacgtcg gcgtggcggt cccaggaggt gcagaagggg
     5821 tcgatcaggg taaggtcgtg cctgctaccc tcgacgagcg ccatatacca gagcgggctc
     5881 ctgtgatgca gcaccgttgc gcccttctcc accttgctgg cgacggcttc gatagtccta
     5941 cgcgccgcgt agtcctcgct gcggtcgtgc tcgacgtagg cttcacgcac acccaagagc
     6001 ggcacgaaaa gcatcaggac cgatagggca acgacaagag cccctctcgc actagagctg
     6061 atcgccaggc cctccactat ccgcagcaag gcaccgagtc ctaacgagat gcacagcccg
     6121 aagaccaaga agaccgggat gaggaacaca tagaagtctt cgatccctag ctgaaggtaa
     6181 acgccggcct ggccgagaca cccgaagaag agcacgccga ggaggatggc cgccgcgcgg
     6241 tccgtgaaga gcaggtagac ggcggccagt gctccgacca ccatcagggc gaaagagaac
     6301 tgcccgagga gatgatctcc gaatagcccc agtttggtcg aggcgtccgc gagcgcgagg
     6361 ctggaagggt tgcattgcct ccccttctca gaagattcgg ccagaaagct accacccgtg
     6421 accagtagca ggaagcgcca gggggtcgaa gggtcagcct cgttcagggg agcgtgcatc
     6481 aaggcccgta tcggaaggta gagcagcgga agcaggccga gcaggaagag tccgacggcc
     6541 ttcagcatca ggcccgtcct cgaaaacacg cgcctgtcgg taaggaaaat gaacgccaga
     6601 gcgccgggga ctagcagaac gctcgtcagg tggtgggtaa gggagaggcc gaccaggaag
     6661 gccgagagca gcaggtatcg gccgacgcga cggtctctcc agacgagcag tacgaagatg
     6721 acgagcccga cgagtaatgc ctccagggag tagacctcgg cgatcacggc ctggctccag
     6781 aaagccccgg agagcccgaa agccagagcg ccggccgccg ctgcaacggc ccgtcttgcc
     6841 agccgcaacc ctgcgaggta cacgaggagc accgccgcga cgccgtagac ggcggaggcg
     6901 aggtttacgc ggtaggctga gtcccccaac ggcaggtaag taaagaggtg cgtcagcatc
     6961 atgtaagtcg ggtatccggt cggatgtccg atcccgagca ccgagacctc ggtctggagc
     7021 atcggggagt cgagcgtgtc gggaacgccg taaggcagga ccgtaggcgc cagggtaccg
     7081 aggtagagga caccgacgaa gaccgcgacc gccgccccga gcaacgccac actccaccgc
     7141 cggggagatc cgatcctgtc gccagagggg ggattcatcg caggggcaga atactacgtc
     7201 ggtgtgccgc tctctccagg caccttctgt atactcggcc cgtggctaag cgcggctaca
     7261 cgcgggacac gatcaggggc ggcgccagcg actgggacgt ctcgcggccc ctttcgagct
     7321 acgctcgtac gttggcgacg ctactcgtgc atccggtgag gttcttcgag ctcttgccaa
     7381 ggattccgga catgagggcg cctaccctct tcctcatctt ctccggcctc cccgccgcga
     7441 tctcgtggtt gctcttttgg gggttctatc ctgcgctggt agcgctcgta ctgccgctac
     7501 cgctctcctt cttcctcgcc ggcctctacc atctcggcgc cctcggtgga cggcacgggt
     7561 acgtggtgac ctggagggtc ctcgcttacc cgcttggttt ctacctaccc ctcgccgctg
     7621 tacctgttct cggacttctg ggagccgctt actcggggct cgtgctcatg gggataggcc
     7681 tcgccgaggt gcaagagatc ggaaggctct gggccgggct cttctgcacg gctgttgtag
     7741 tcgtgttcgg ggcggcttac tacttttcga gccttgcctg atccggactc gagcgaggat
     7801 tcattcttcg ggcgggtctt cctgggatcc tacgtactcc agcgagacca cgtcggcctc
     7861 gacgaagccg agtgagcggt agaattcgcg ggccgttacg ttcgcgcgtg tggccaccag
     7921 ttcgatctgt gatgctccgt tgtcggaagc tacgttgcgc acctcctcca tgaggagctt
     7981 gcctaccccg gttcgcctgt ggtcctcccc cacgacgagc atgggcacct cgacgacggt
     8041 gtcgccgtgg gcgaggtcgg gtttgatcca aaagctcacc ccacccacga tcccggcctc
     8101 gttctcggcg acgaggaccc gagccctggg ttcgtccagg agctcctcga gccgcgccct
     8161 gacggcctct tccgtgggcg gctcatcgcc taccttttcg gccagctcgc ggacgagaat
     8221 atgtatcctc gcgacgtcct cggcggtcgc ttctcgcgtc ttgatcttgg atttggcgtc
     8281 catgctactc ctcggttcac catctggcgt attacttacc ctacgtagcc aggcgacgaa
     8341 cggatgaagt cgctaagatg atccagacgc ggaaaggagt agcatgggat gcaggacggt
     8401 cgtgatcgtg ctcgacgggc tcggcgctgg caatgccccg gacgcggcgg acttcggcga
     8461 cgagggcgcg aataccttat cgaacacggc gaaggctaca ggtggtatcg aagcgccgaa
     8521 cctccagacc ctcgggctcg gtaacatcgg ggagatagaa ggcatatctc ccacctcctc
     8581 tcccgcggct tcccacggcc tgatggtcga aatgtcggcg gcaaaggcca ccctggcagg
     8641 acactgggag atgatgggcc tcgtgctcga agacccgctc cccacctatc cagaaggctt
     8701 cccggaagag atactgcgcc gcttcgagaa cgagacgggg cgcggggtca tagggaacag
     8761 gccggcctcg ggaacccgga tcatcgagga gctcggtccc gagcaggaga agacggggaa
     8821 ctggatcctc tatacctccg ccgactccgt cttccagatc gccgcgcata cccgcgtgat
     8881 cccactcgat gaactgtacg aagcctccaa aaaggcgcac aagatgctga tcgaggaggg
     8941 cgagatcatg gtcgaacggg tcatcgcccg tcccttccac ggcgagtccg gcgcctacgt
     9001 ccgcgaaaac gagaaccgcc acgactacgg cataaccccg ccctctgaga cctatctgga
     9061 ccgcataaag gttgcgggcc acgaggtagt cgcggtcggc aagatcaggg acatcttcga
     9121 cggtcggggc ataacagagc atctacccgc tccgcccgac gatgcagcca aagtgggcgc
     9181 catcctgcag gcactaggcc gtgtcgagtc tggcctgatc ttcgcaaacc tcgttgattt
     9241 cgacgcgaag tacggacacc gccgcgatcc cgagggaatg gcggagaaca tctctcgctt
     9301 cgacgctcgc ctgcctgagt tgctcgacgc catcggtccc aacgacctcc tcatcataac
     9361 cgccgaccac ggcaacgacc caaccttcaa cggaacggac cacacccgcg agagggtccc
     9421 gctcctcgtc gtcggcggtg gggagccgct tgacctcggc gttcgcgagg gcttctccga
     9481 tgtcggagcc tccctcgccg cgtggctggg tgtcgaaaag ggcaggcttc caggagtgga
     9541 tcttctgaag tgaacgcccg accccaaagc cactctgctc ctcctgcgca tctatcggat
     9601 ggaacatgag gagatcggcg cattcgacat cttcctcgtc cccatcggcc cggacgagga
     9661 agggctctgc tacgaggcta ttttcaactg agcgtctcta cccgatgatg ccctcgcgct
     9721 tctcctcctc gtagaccctg tctgccacga actgccgcat cagggtctgg taaccgacgc
     9781 ctttgacggc ggccagtacc ttgaggcgat tcaccaggtc gttctccatc ctcacggaga
     9841 ccgggttctt gcgagggcgc gttatggtcc gctctcgtgg tggcggcaga ttgctcctct
     9901 ccgagcgtgg ctcaaacgtc tccagcagtt cctccgagat gtcatgctca tcccaccacg
     9961 cctgctcttc tttctcggac gcgaaggacg gcacttcatc ccaactcttt atctccttcc
    10021 tccctttctg catgccttca cctcctcttt cgcctgtatc ttcgcttctg tgcatcgctc
    10081 gcgtctctcg ccgttatcgg gcgggtcctc ttgccacatc tcgtgtagac aacgaggagg
    10141 atacgcccct cctcggtggc gcctaccaat gcctctcgtg tctctcctgg agcatcataa
    10201 gcgtcaacgc cgaaaccctg ggggtccagc aatgcttcct ctacttccgt cgggtcaaca
    10261 ccatgaccca gcacatgccc ctcgttgccc tcatcccaat cgaaccagcc accatctccc
    10321 acgcttagag tctaacaaat gtacatacga atgtgtgagc tgaacaggcg ttacccgatg
    10381 cagcggcgag tggctagctt cttcctcgcc aggctcgaca ggccgaagtg ttacgcccgg
    10441 ctcacggcgt cgaacgcatt tccagatgcg caaggtccac atccgctccg gcacgaacca
    10501 gaaagtccat ttgcaggatg ccctggatct cgaagccaca gtccatcgcg ccgacttcga
    10561 tctcaattgc agtccacccg cttggtaaaa acaaactcta tgccaccgcc cacgcggcga
    10621 ataggatgag aaccgtcctg ggcttcgtag cgcaggccaa gtctcaagac tttgtctgcg
    10681 gcgaatacgg tctctgcgca gccggtgtcc aatactacgc aaaaaatcga gtgcttcgat
    10741 gagaggcttc cggagctgct cgatgcactg actgcagcag acctcctgat catcagtgca
    10801 gaccacggca acgaccctac cttcaaagga acggagccgt cgccgcttcc gttcgtcgat
    10861 cctgggttga ggccgccggc gccgttccca acgagctgct caacacccgg ggaggaggtc
    10921 gcggccattc cctcgatccc gaagtc
//
LOCUS       DATMVF010000165         8861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_486020, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000165 DATMVF010000000
VERSION     DATMVF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486020"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            972..2768
                     /locus_tag="VN178_08255"
     CDS             972..2768
                     /locus_tag="VN178_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWS81003.1"
                     /translation="MKTLRCEVLSFPKQVREILKKSERAVQVALDVQSEGPGIVGAIL
                     AGVSICFEPGTAYLAFLGSEDTNSEGMLQQLRPLLGADGPPKVMHDAKPGLMVLAQRG
                     VETGTPAFDTLLAAFLLDGNAYDLEDLMADRLGLPAEESETIESEERIRHICARADAV
                     LRLGGLLEAELEERGQLGYLRELELQLVPVLADMELAGVAVDATTLEELSHRLAVRLH
                     ALEKEMAEAVGHEVNPRSPQRIGELLYDELGLEGSRKTSSGRYSTDALALAELVGQHP
                     VVEKIVEHRRLSRLKDAYADSLPGLVNPQTGRVHTSFSQVSASSGRLVSREPNLQNIP
                     VRTQLGREIRSAFVADASGLGIRGPTVLLSADYSQIELRVLAHMTGEPALREAFRKDE
                     DVHALAAAGLYGVPPEEVTAEMRRVGKMLNYGVVYGMTGYRLALEAGIDRKEAARFVR
                     RHREQYPAVRAYVERTLREAEEKGYVGTPLGRRRYLPDIGAKNRQRREAARRAAENMP
                     NQGMVAEIIKLAMIGVHARLREEGMATRMLVQVHDELVLEVPEVELAEAAGLVVREMK
                     HAVELSVPLEVEVKIGRSWGELRNLGDPRVNQ"
     gene            complement(2785..4533)
                     /locus_tag="VN178_08260"
     CDS             complement(2785..4533)
                     /locus_tag="VN178_08260"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWS81004.1"
                     /translation="MRFSVLVQYFRRLEGTSGRNALVEVLAELFARTPTEDVPPTAYL
                     LQGRLAPYFEPVEIGLGNNYVADAIAWAFGGDRATVLERFDRLGDIGEAAAEIAAESG
                     SSPAPNRAAPVRGIFDRLLKIARTTGAGSVRAKVEAFASLLGELDPLSCNYLCRIPLG
                     RMRLGVGDPTVLDAFSFARVGDKSFRKRLERAYNETSDLGLVGKTLWEGGIEAVEHLG
                     VRVGNPVRPALAERLPSAEAIIAKLGRAAVERKYDGFRCQVHKAGGDIKIFSRNLEDM
                     TASFPEIAEGARRQVVASTAIFEGEALAYNPLSDEYLPFQQTVRRRRKHGVEETAAEL
                     PLRLFAFDVLYADGERVMDRSYTERHAILEDAVEAGDTILISEAHTTSEPEELLEVFK
                     DSIQHGLEGIVAKRPDSPYQAGARNYNWVKLKRAQAGNLQDTVDGVIVGYIYGRGRRT
                     VFGVGALLVATYDPERDVFPTVTKIGTGLSDAQWREVKERCEPYVSERKPARVESNIV
                     PSVWVEPQVVIEMLADEITRSPVHPTGTDEGGQGYALRFPRLVSFRQEDKKPEDVTTV
                     AEIVELYNQQGARTTR"
     gene            complement(4772..5956)
                     /locus_tag="VN178_08265"
     CDS             complement(4772..5956)
                     /locus_tag="VN178_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWS81005.1"
                     /translation="MGIEHTEYKARQAAARKLVQERGFSAIVAWSRGGSTQHHYADVY
                     YLAGFYTHQPFIPDEPGRWRAHGHTALVLPVDGPGTLLVDMAEVQNPKPAADRVRVTA
                     DLVESLATTVMDTIPPGTDVALLGGETLVWRWGRELNTRLPGYRFLETDDLAAEMRLI
                     KSEAELDLLRAAGEIGARAVDAAMQAAIPGVSEAEVAAAAISEIVRAGGALYGLGLSS
                     GPWAHTFSPSTPAPYSRRHLEAGDMIRLDLYGSFDGYLFDFGRSRVVGRSPTQEQQEI
                     LDAVRDSVQAGTELLRPGETLGAVARRCEEVLMASAYARRHGLPCAMMGGTWGHSLGL
                     DWGTPWLESASSTVIQPGMCFAIERRIEAPDIGGANYENDVIVTDDGPEIVTPAHDCY
                     GE"
     gene            complement(6095..7228)
                     /locus_tag="VN178_08270"
     CDS             complement(6095..7228)
                     /locus_tag="VN178_08270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWS81006.1"
                     /translation="MISNSFQRGRFALALRTARSLLGGATGLEQPREPYVQGVTESSA
                     VICWVSEQPGSGVVEYGKTLELRRKEAEARIGGRHAVALTGLDPGSTYHYRVKGIGGS
                     SATGCFRTAPAEEDPRFSFAVVGDSGSGGKGQLAVAALLERLRPDLILHTGDVVYPTG
                     QERYYDRRFFTPYRNLIKTVPLYPVLGNHDVRKGNGAAFLENFYPPLSSPGSTKRHYS
                     FDWGNIHIVALDSELYHGDTGSDPEDQRDFLERDLATTRKRWRIVFLHRSPYGSSRHG
                     GDEKVREGLEPLFVKYGVDLVFSGHDHVYERTVPIKGVTYVVSGGGGRRLYRTGKGEL
                     TASSVSAHHAVLVRVDGRCLLLEAVEAGGKVVDRFDLYKPHAK"
     gene            complement(7313..7996)
                     /locus_tag="VN178_08275"
     CDS             complement(7313..7996)
                     /locus_tag="VN178_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81007.1"
                     /translation="MPSKEAQTKGHLRACPVQVTGSAPVGLQPLMLGAARDSYLYVPA
                     SYRAARPAPLVLLLHGAGGHARQGLDLLRSLADAAGLLLLAPASRDHTWDLLVDRCYG
                     PDAGMIDRALEQTFSRYAIDPERLAIGGFSDGASYALSLAISNGALFTHVIAFSPGFV
                     APAGHTGSPRIFVSHGTRDGVLPIDRCSRRIVPELERGGYEVLYREFEGGHTILPEIA
                     SEAVGWFTK"
     gene            complement(8168..8713)
                     /locus_tag="VN178_08280"
     CDS             complement(8168..8713)
                     /locus_tag="VN178_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS81008.1"
                     /translation="MPEIGERVQNVRRLRMWTQARLAKEAGVSPTTVSGIETGKIGRP
                     HFGTIGKLALALGVAPEELLDSREASEQQGPAPLSLGWARESGEGEFESALEGASLKS
                     LDSLSRGLDVEQERLQRLYGEFPEGSEPGRFIKRQIREVAGRSGSVKASAMVRRNQDD
                     AKRTDEHGRVAESTMDEPQAL"
BASE COUNT         1635 a         2786 c         2811 g         1629 t
ORIGIN      
        1 acaagtgcaa tagtgcaggg gggtgcacgg aaggcagctg ctgtggcggt acgtgctgtg
       61 gtcaaggaga ggaaggtact cctcctgcgc aatttgtgtc gtgtgttcct gcagcgtcag
      121 tgtgcggagg aagggttgga acagggtgct accatacgat cgaagcgccc agacagccta
      181 cctgccttgc aaattgcggg ggcgggatcg cctgcacagc aagcgcggag tgccccagcg
      241 gcatggtatg tgctcaacag gaaggctcct cctcgggcta cacctgcatt tcactctgta
      301 gtaagggcgg gggaagaagg taggtagcac tatatcatcc cttgggtacc tcgctcagga
      361 cagagagcta tccaagaggg tagctctctg tgtggcacac ctagcttacc gcttagatct
      421 ccggtgcgcg gtcttgtcgt agggccaggg atagtccttg gccctgcctc ttgcccttat
      481 tcaccgaagt gcgtagaata cgttttctcg gagattcata tccaagatcc tgcatagccg
      541 cgccccgata ccccgcaatg tccccctcca ggcaccctgc atagccgcgc cccgctaccg
      601 cttgtaacac tgatggatag atatgcgcga tcatggatgt cctttctaca acttaagctt
      661 gcacttcgga gagaacaccc catgccccat cgctaatgct ccaggtagcg ggagcccggc
      721 tccataatta ccatcaacgt ttagtaacta cacacactct gagccaaggt tccgtgccct
      781 gctgaccgaa ggagcctcag ggtggacgct cgtctagcgc ttacgcgctc gtggcctgct
      841 caccgaactg cgtacaaaaa ccacaacggc ggcacgccac ggcaacgggc agcgattaca
      901 atggtcgcat ggggacgact actgccgacg gagagtgaga cgatgagtct ggtcagacaa
      961 actcgccgga catgaaaacg ttgcgctgcg aggtgctctc gttcccaaaa caggtgcgcg
     1021 aaatcctgaa gaagtccgag cgcgcagtac aagtagccct cgacgtccag agcgaggggc
     1081 cggggatagt tggagccata ctcgctggcg tttcgatctg cttcgaaccc ggcaccgcct
     1141 acctcgcgtt tctaggctcc gaagatacca acagcgaggg gatgctccag caacttcgcc
     1201 cgctactagg cgcagacggt ccgccaaagg tgatgcacga cgccaagccc ggtctcatgg
     1261 tgctcgccca gcgtggcgtg gagactggaa cacccgcctt cgatactctg cttgcagcgt
     1321 tcctgctcga cgggaacgcg tacgatctcg aggacctgat ggccgatagg cttggtctgc
     1381 cggcggagga aagtgagaca atagaaagcg aggaaaggat acgacacatc tgcgcccggg
     1441 ccgatgcggt gctgcgcctc ggcggactcc tcgaggcgga gctagaggag cgggggcagc
     1501 tcgggtatct gcgggagctc gagctgcaac tcgtccccgt actcgccgac atggaactgg
     1561 caggcgtcgc cgttgatgcc actacgctgg aggagctgtc gcaccgcctg gcggtgaggc
     1621 tgcacgcact tgagaaggag atggcggagg cggttgggca tgaggtaaat ccgagatcgc
     1681 cacagcggat cggagagctt ctgtacgacg aactgggcct ggaggggagc aggaagacct
     1741 cctctgggcg ctactctacg gacgcgctcg cgctcgcgga gctagtcggc cagcacccgg
     1801 ttgtagagaa gatagtggag caccgtcgcc tcagtagact caaggacgcc tacgccgact
     1861 cgctccccgg tctcgtaaac ccgcagacgg gacgcgtgca cacgagcttc agccaggtct
     1921 cggcgagcag tggccgtctc gtctccaggg aacctaacct ccagaacatc cccgtgcgca
     1981 cgcagctggg gcgcgagatc cgcagcgcgt ttgtagccga cgcctcgggc ctcggaataa
     2041 gagggccgac cgtgttgctt agtgcggatt actcccagat agagcttcgg gtgctggcac
     2101 acatgaccgg ggagccggcg ctgcgtgagg ccttccggaa ggacgaggac gtccatgccc
     2161 tcgccgccgc cggcctctac ggtgtgccgc cagaagaagt caccgccgag atgcgtcggg
     2221 tcggcaagat gctcaactat ggcgtcgtct acgggatgac cggctacagg ctcgcgctcg
     2281 aggcgggcat cgaccggaag gaggccgcac gtttcgtcag gcgccaccgg gagcagtacc
     2341 ccgccgtgcg ggcctacgtc gagaggacac tgcgcgaagc agaggagaag ggctacgtgg
     2401 ggactccctt ggggaggcgt cggtatctgc cggacatcgg agccaaaaac aggcagcgcc
     2461 gggaggcagc acggcgggct gcagagaaca tgccgaacca gggcatggtg gcggagatca
     2521 tcaagctggc gatgatcggg gtgcacgcgc ggctgcgaga ggagggtatg gcgacgcgca
     2581 tgctggtgca ggttcatgac gagctcgtcc tagaggtccc cgaagtggaa ctggccgagg
     2641 cggccgggct ggtggtacgc gagatgaaac acgctgtgga gctctccgtg ccgctggaag
     2701 tcgaggtcaa gataggacga tcgtggggag aactacgaaa tctaggcgat ccccgggtga
     2761 accagtagcg ggacgacgcc acacttaccg cgtcgtccgc gccccctgct ggttgtacag
     2821 ctccacgatc tcggcgaccg tggtaacgtc ttcgggtttc ttgtcctcct ggcggaaact
     2881 aacgaggcga gggaaccgta aagcgtagcc ctgaccccct tcgtccgttc ccgtagggtg
     2941 cacaggcgag cgggtgatct cgtcagcgag catctcgata accacctgcg gttcaaccca
     3001 cacgctgggc actatgttgc tctcgacgcg cgcaggcttt ctctcgctaa cgtacggctc
     3061 gcagcgctct ttcacctcgc gccactgggc gtcgctcaag ccggtcccga tcttggttac
     3121 ggtcgggaag acgtcgcgct cagggtcgta ggtggcaacg aggagtgcgc ccacgccgaa
     3181 cacggtccgc cgtccccggc cgtagatgta gccgacgatc acgccgtcaa cggtatcctg
     3241 gaggttgccc gcctgtgccc gcttgagctt gacccagttg tagttgcgcg ccccagcctg
     3301 gtagggtgag tcgggcctct tggcgacgat gccctccaag ccgtgctgga tggaatcttt
     3361 gaacacctcg agcagctcct ccggttcgct ggtcgtgtgg gcttcggaga tgaggatggt
     3421 atcccccgct tcaaccgcat cttctaggat ggcgtgcctc tcggtgtagg atcgatccat
     3481 gactctctcg ccatcggcgt agaggacgtc gaaggcgaag agcctcaggg gcagctcggc
     3541 cgcggtctcc tcgacgccgt gcttgcgtct cctcctgacg gtctgctgga acggaagata
     3601 ttcgtcggag agcgggttgt acgcgagcgc ctcgccctcg aatattgccg tgctggcgac
     3661 gacctgccgt ctcgcgccct cggcgatctc cgggaatgag gccgtcatgt cctcgaggtt
     3721 gcgtgagaag atcttgatgt cgccaccggc cttgtgcacc tggcatcgga aaccgtcgta
     3781 cttcctctcg accgctgccc gcccgagctt cgcaatgatc gcctccgcgc tcggcagccg
     3841 ctcggcgagc gccggacgca cagggttgcc gacgcggacg ccgaggtgct ctaccgcctc
     3901 tatgccgccc tcccacagcg tcttccccac gagcccaagg tcggaggttt cgttatacgc
     3961 gcgctcgagc cgctttctga agctcttgtc gccgactctg gcgaaggaga acgcatcgag
     4021 cacagtgggg tctcccacgc ccaagcgcat tcggcccagg ggtatgcggc acaggtagtt
     4081 gcacgacagc ggatcgagct ccccgagcag cgatgcgaac gcctcgacct tggccctgac
     4141 gctgccggca cccgtcgtgc gcgctatctt gaggagtctg tcgaagatcc cacggaccgg
     4201 agcggcccta ttaggagccg gggaggaacc agactccgct gctatctcgg ccgctgcctc
     4261 cccaatatcg ccgagacggt cgaaacgctc gagcaccgtg gcccggtcgc caccgaaggc
     4321 ccaggcgatg gcgtcggcca cgtaattatt gccgagtccg atctcgacag gctcgaagta
     4381 gggcgcgagt ctgccctgca agagatacgc tgtggggggc acatcctccg tcggggtcct
     4441 cgcgaagagc tcggctagga cctcgaccag ggcgttgcgc ccagacgtcc cttccagcct
     4501 ccggaagtac tgcacgagca cagagaaacg cacgacgtat cctttcgttc gggatcgcgt
     4561 cattttaccg gcagtcgcgg cgagaggagt acaaggcagg cggccgctac acgaggcctc
     4621 aaacggtcgt caacgttccg aaaccacaca ctctgagcca aggttccgtg ccttgctgac
     4681 caatacagct tccgtgaaga agtcggtccg gtggttagcc ctctcgagaa gctaagagat
     4741 gccagccgtg cggtcgagcg cctggtactt gttactcgcc gtagcagtcg tgggcggggg
     4801 tgacgatctc gggaccatcg tcggtgacga ttacgtcgtt ctcgtagttg gcgccgccga
     4861 tgtccggcgc ctcgatgcgc cgctcgatgg cgaagcacat gcccggttgg atgacggtag
     4921 aggacgccga ctcgagccac ggcgtgcccc agtcgagccc gaggctgtgt ccccacgttc
     4981 cgcccatcat tgcgcacggt aggccatgac ggcgggcgta ggccgaggcc atgagcacct
     5041 cctcgcagcg ccgggcgacg gcgccgagcg tctcgccggg tcgcagcaac tccgtgcccg
     5101 cctggacgct gtctcgaacg gcgtcgagga tctcctgctg ctcttgcgtg ggcgatcgac
     5161 ctacgacacg cgagcggccg aagtcgaaga ggtagccgtc gaacgatccg tacagatcga
     5221 ggcgaatcat gtcgccggct tccaagtggc gacggctgta gggcgcgggt gtagaagggc
     5281 taaacgtgtg cgcccacggg ccagaggaga gccctagtcc gtatagtgct ccgcccgcgc
     5341 gaacgatctc cgagattgcc gccgcggcga cctcggcttc actaacaccc gggatcgcgg
     5401 cttgcatggc ggcatcgacc gccctagcac cgatttcgcc agcggcgcgc agcaggtcga
     5461 gctccgcctc gctcttgatt agtcgcatct ccgcagcgag atcgtcggtt tcgaggaacc
     5521 ggtacccggg gaggcgggta ttgagctcgc ggccccaccg ccatacaagc gtctcgccgc
     5581 cgagaagcgc aacgtccgtg ccagggggaa tcgtatccat tacagttgtt gcgagcgatt
     5641 cgaccagatc cgctgtgacg cgaacgcggt cagcagccgg cttcgggttc tggacttcgg
     5701 ccatgtccac cagcagcgtt cccggcccgt ccacggggag caccaaggcg gtgtggccat
     5761 gcgcgcgcca gcgcccgggc tcgtcaggga tgaagggctg gtgggtatag aagccggcga
     5821 ggtagtagac gtcggcgtag tgatgctggg tggaaccccc gcgggaccag gcgacgatgg
     5881 cggagaagcc gcgctcttgg acgagcttac gggcggccgc ctggcgagct ttgtattcgg
     5941 tgtgctcgat ccccacggct gtctctactt tcgcttttct tgcggcgttg tgctagcgga
     6001 tgacgccgcg ctcccgccta gtggtggctc tgtgcttggt ggagtatagc tgcctcggag
     6061 agggctatga acggcttgac tcttcgcgag agggtcactt cgcgtggggc ttgtagaggt
     6121 caaagcggtc aacgaccttg ccgcccgctt ctacagcctc cagcaacagg catctgccgt
     6181 cgacgcgcac gagcaccgcg tggtgggcgg acacggagga ggccgttagc tcacccttgc
     6241 ccgtccggta cagcctcctg ccacctcccc cgctcaccac gtatgtaaca ccttttatgg
     6301 gcacggtgcg ctcgtagacg tggtcgtggc cagagaaaac taagtccacc ccatatttca
     6361 cgaagagcgg ttccaagcct tccctcacct tctcgtcacc gccgtgtcta gaggagccat
     6421 agggagacct gtgtaagaac acgatcctcc agcgcttgcg cgtggtggct aggtcgcgct
     6481 ctaggaaatc cctctggtcc tccgggtcag aacccgtatc accgtgatac agctcgctgt
     6541 cgagcgcgac tatgtggatg ttgccccaat cgaaggagta gtggcgcttg gtgctgcccg
     6601 ggctgctgag gggcggatag aagttttcga ggaaggcggc gccgttcccc ttcctcacgt
     6661 cgtggttgcc gaggaccggg tataggggca cagtcttgat gaggtttcgg tacggagtga
     6721 agaagcggcg gtcgtagtag cgctcctggc ctgtcgggta gaccacgtcg cccgtatgca
     6781 ggatcaggtc cggcctcagg cgttccagca gcgcggccac tgccagctgt cccttccccc
     6841 cgctgccgct gtcgccgacc accgcgaagc tgaagcgtgg gtcctcttcc gcgggggcgg
     6901 tgcggaagca ccccgtcgcc gacgagccgc caatgccttt cacgcggtag tggtaagtcg
     6961 agccgggatc gagacccgtg agcgcgaccg cgtggcgccc accgattcgg gcctcggctt
     7021 ccttgcgtct cagttcgagg gttttgccgt actcgaccac gcccgatcca ggctgctcgc
     7081 tcacccaaca gatgactgcc gaggactccg tgacgccctg cacgtaaggc tctcgcggct
     7141 gttctaaccc tgtggcacca ccgaggagag accgcgcggt ccgcagcgca agtgcgaacc
     7201 ggcctcgttg gaaggagttt gagatcattt cacctatgct acccggtgct gctggcggag
     7261 cgcgaggagg tccggccccg gaagggcgct gaccggtctc ttaggggaac ttctatttcg
     7321 taaaccagcc aaccgcctct gaggctatct ctggcaagat cgtgtgccca ccttcgaatt
     7381 ccctgtagag cacttcgtag ccgccgcgct ccaactcagg aactatcctg cgactgcagc
     7441 ggtctatggg cagcaccccg tcgcgcgtgc cgtgggagac gaagatccta ggcgagccgg
     7501 tgtgcccggc aggagccacg aaccccggcg agaaggcgat cacgtgcgtg aagagggctc
     7561 cgtttgagat cgccaaagag agcgcgtagg aggccccatc cgagaaaccc ccgatggcca
     7621 gacgctccgg atcgatggcg tagcgagaga acgtctgctc aagcgcccgg tcaatcatgc
     7681 ccgcgtcggg gccgtaacag cggtcgacga gcaggtccca cgtgtgatcg cgcgaagcag
     7741 gggccagtaa cagcagaccg gctgcgtccg ccagactccg cagcaagtcc aggccctgtc
     7801 gcgcgtgtcc tcccgcgccg tgcagcaaga gcaccaacgg ggcgggacga gctgcacggt
     7861 agctcgccgg cacgtacagg tagctgtcgc gcgcggcacc gagcatcagc ggttgcaggc
     7921 cgaccggcgc ggacccggtt acctgcactg gacacgcccg cagatgtccc tttgtctgcg
     7981 cctccttact cggcatggac cttctattcc cgcgccattc ttcggctaca ccgcggcgac
     8041 gctgcatctc tttggcacag ccgcggtcgg cggtgctcca agggatacat gagtcccatt
     8101 acacccagtc gcccagtcta cgattccgcg ccttgctgac cgagggtggc gaccggggag
     8161 tgcaacttca cagagcctga ggttcgtcca tggtggattc tgcgaccctg ccgtgctcgt
     8221 ccgtacgctt tgcgtcatcc tggttgcggc gaaccatagc agaagctttc accgaccccg
     8281 accgccccgc tacctccctg atctgacgct ttatgaaccg tcccggctca ctgccctcgg
     8341 gaaattcccc gtaaagcctc tgaaggcgct cttgctcgac atctagtccg cgagagaggg
     8401 agtccaaact cttgagggaa gcaccctcaa gagcgctctc gaactcgccc tccccgctct
     8461 ctctagccca ccccagcgac aggggcgcgg gaccttgctg ctcgctggcc tcccgcgaat
     8521 cgaggagttc ttcgggggcc acgcccaatg ctagagcaag cttaccaatg gtgccaaagt
     8581 gcggacgccc gatcttgccg gtctcgatgc cggagaccgt ggtgggactt actccagcct
     8641 ccttagctaa cctcgcctgg gtccacatcc gcaaccgacg cacattttgt actcgctcgc
     8701 ctatctctgg cacgttcaca cctcgtctct atcaatcagg gaaagcatac cataaactgt
     8761 atcgaaactc tgtagttact gtgtttggct agaagaagtc tgtaaatttc tcggtaaaac
     8821 tgtttgaaaa ctgtgtcaga gggtatataa tggtcgcgca g
//
LOCUS       DATMVF010000166         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_661220, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000166 DATMVF010000000
VERSION     DATMVF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661220"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..165
                     /locus_tag="VN178_08285"
                     /pseudo
     CDS             1..165
                     /locus_tag="VN178_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            236..1129
                     /locus_tag="VN178_08290"
     CDS             236..1129
                     /locus_tag="VN178_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWS81009.1"
                     /translation="MTDEPRAGDQDTLTWAQETIARQAEEIERLRRQLADERFAENLR
                     ETLTLAVAVGTIASPVTHSRLLEMIVETAAHVISARAAALFLIDEEAQELVFEVALGS
                     KAEEVKKFRVPLGHGIAGLVAMTGQPMAISGAEGDPRQAADIARSVGYTPQSILCVPL
                     FYHDQIIGVLELLDKERAPSFSTTDIEDLGLFANQAAVAIEQSRTNRNLAALFGEVLK
                     SIGGVTDGQRQELQERARAFAEHLEEDTAFRQALDLARLVQEIAHQGENELRGCQAIL
                     RGFAEYLRSRPEPVSGLRDML"
     gene            1126..2013
                     /locus_tag="VN178_08295"
     CDS             1126..2013
                     /locus_tag="VN178_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWS81010.1"
                     /translation="MRERLPAWSSAFASDALQMLRPIQPIDDIDREWAWGGSSGQGVK
                     VAVIDSGVDATHPAVGRVDGYVAISEGPDGLAYDTEPHEDVFGHGNACAGIIRALAPD
                     CEIYSVRVLGPRLSGRGSVFAAGLRWAIENGMQVCNLSLSTSKKDFFGVLHELADQAY
                     FAHVMLVAAANNMPGASYPATYASVFSVASHDIEDPYLFYYNPKPPVEFGAYGIDVRV
                     AWANGGWQTATGNSFATPHIAGLVAKILSKHPDLTIFQMKAVLRALAANVSRDQAGRE
                     RPETEPIEAGNKEPPLRRR"
BASE COUNT          454 a          694 c          749 g          403 t
ORIGIN      
        1 tacacacgac cctatgaccc gcgctaccct caagtctgta tggatgaggt ctccaagcaa
       61 ctgctgcggg atacccacca acccctgccg atggagccgg gaagacccca acggcgagac
      121 tatgagtatg agcggggtgg gagggtgaat ttgttcctgt tctgcaaccg attcctggtt
      181 gacagcctgt aattcttgat tatatgtttt gtgtacagga aaaggagatt gtttcatgac
      241 agacgaacct cgagcaggag accaagacac actgacatgg gcccaggaga ccatcgcacg
      301 gcaggcggag gagatcgagc gcctgaggcg ccagctcgcg gacgagcggt tcgccgagaa
      361 tctgcgggag acgctaaccc tagccgttgc ggtgggtacc atcgcctcgc cggtcacgca
      421 ttcgcggctc ttggagatga tcgtcgagac ggcggcgcac gtgatctccg cgcgagctgc
      481 cgccctcttc ctcatagacg aggaggcgca ggagctggtc ttcgaagtgg ccctgggatc
      541 gaaggccgaa gaggtgaaga agtttcgtgt gcctctcgga cacggcattg ccgggctcgt
      601 cgctatgacc ggtcagccga tggcaatctc gggtgctgag ggcgacccgc gccaggctgc
      661 cgacatcgcc cgtagcgtcg gctacacgcc gcagagtatc ctctgcgttc ccctcttcta
      721 ccacgatcag atcattggcg tcctcgagct cctggacaag gagagggccc cctctttcag
      781 cactacggac atagaggacc taggactctt cgcgaaccag gctgccgtcg ccatcgagca
      841 gtcccggacc aaccggaacc tcgcggcgct cttcggggag gtgctgaaat ctatcggcgg
      901 agtaaccgac ggccagaggc aggagctgca agaacgcgca cgggccttcg cggagcacct
      961 tgaagaagat accgcctttc gtcaggcgct cgatctggcc cggctcgtgc aggagattgc
     1021 ccaccaaggg gagaacgagc tgaggggctg ccaggcgatc ctacggggct tcgcagagta
     1081 tctgcgctcg aggccggagc cggttagcgg actacgggac atgctatgag ggagcggcta
     1141 cccgcctggt cctcggcgtt cgcctccgat gccctgcaga tgctcaggcc gatccagcct
     1201 atagacgaca tagaccgaga gtgggcctgg ggaggtagca gcggccaggg cgtcaaggta
     1261 gccgtgatcg actccggcgt cgacgctacc cacccggccg ttggccgggt tgacgggtac
     1321 gtggccatca gcgaaggacc cgacgggctc gcctatgaca cagagccgca cgaggacgtc
     1381 ttcggtcacg gcaacgcttg tgccggcatc attcgagccc tcgcgccgga ttgcgaaatc
     1441 tacagcgtca gggtcttagg accgaggctc tctggccggg gtagcgtctt tgccgctggg
     1501 ctgcgctggg ctatcgagaa cgggatgcag gtgtgcaacc tatccttgag cacctcgaag
     1561 aaggattttt tcggcgtgct acacgagctg gccgaccagg cctacttcgc ccacgtgatg
     1621 ctggtagcgg cagcgaacaa catgcccggt gccagctatc ccgccacgta cgcctccgtc
     1681 ttctcggtcg cctcgcacga catcgaggat ccgtacctct tctactacaa cccgaagccc
     1741 ccggtagagt tcggagcgta cgggatcgac gtgcgggtgg cctgggcgaa cgggggatgg
     1801 caaacggcga ccgggaacag cttcgctaca cctcacatag cgggcctagt agcgaagatc
     1861 ctgagcaaac acccggatct gacgatcttt cagatgaagg ccgtcctccg ggccctggcc
     1921 gccaacgtat cgcgcgacca agccgggcgg gagcgcccgg aaacggagcc gatcgaggcc
     1981 ggaaataagg agcctccgct gcggcgccgg taggtagaag aggagtaaga ggatgagcgc
     2041 tctacattac catcgggcgg cgggggccac gccgcgttgc gggccggcag gtgaccaccc
     2101 ggctccgtcg ccagaccgcg gtaaggtgcg gggaggttta ttatgagcac tacttccggc
     2161 agcgatccac acgggtcttc gcagtggcag aagcaccagt cggcgcactt cgtcttctgg
     2221 tttatgcctg gttcccaagc tgagaagaac gtgacggctt tagcttccag gctggaagcc
     2281 gtccgggacg tgacggtgga
//
LOCUS       DATMVF010000167        12340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_163899, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000167 DATMVF010000000
VERSION     DATMVF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163899"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VN178_08300"
     CDS             <1..1101
                     /locus_tag="VN178_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS81011.1"
                     /translation="DALGRARGAFPGAGGHDEREQTLNQLLVEMDGFDSKSGIIMLAA
                     TNRADILDPALLRPGRFDRQIVVDRPDLPGREKILRVHTRGKPLGPDVEVGTLARGTP
                     GFTGADLANLVNEAALLAARHDKNQIDMSEMEEAIDRVIAGPERKTRLISDKEKEITA
                     YHEGGHAIVGALLPDADPVHKITIIPRGQALGVTMSLPTEDRFMMSRGQLMAQLAMML
                     GGRAAERVVFDEITTGASNDLERVTQTARQMVTRFGMSEKLGPMALGHQQGQVFMGRD
                     FNNQPDYSDEIAFQIDKEIRRIVDESYDTAEDVLVRNRELLDKLSAELIEYETVDANH
                     LKRLIEEYAVDSISPNGAVPDSDGRYRGRYQD"
     gene            1102..1962
                     /gene="folP"
                     /locus_tag="VN178_08305"
     CDS             1102..1962
                     /gene="folP"
                     /locus_tag="VN178_08305"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWS81012.1"
                     /translation="MGRTARNTDGAGTLTTGPGPVVVGILNVTPDSFSDGGDFLDPEA
                     AARRAASMLDEGAGMLDVGGESTRPGSDPVSQEEEMRRVIPVIGGILAARPEAVISVD
                     TYRSGTAAAALEAGARLVNDVTALRGDPSMASVVGEAGCPVILMHMQGEPKTMQKEPH
                     YEDVVREVRDFLAERAGYAVDAGVRPENIIVDPGIGFGKNLQHNLDLLNNLDAIVDLG
                     FPVLIGASRKRFIEGITGVQEARNRVPGTVATTVLAYERGATFFRVHDVRANREALAV
                     AEAVLDTSRR"
     gene            1959..2462
                     /gene="folK"
                     /locus_tag="VN178_08310"
     CDS             1959..2462
                     /gene="folK"
                     /locus_tag="VN178_08310"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWS81013.1"
                     /translation="MSKVFLSLGSNLGDRLAHLRAVVAALGRGPRMRVAGISKVYETV
                     PVEVEEEQPDYLNCVVELECGVPAIELLRYCQGIEAALGRERAEAGEKAPRTADIDVL
                     LFGEETIVEPDLRVPHTGVTRAFNLRGLADLDANLYIPQRGTVGELLESSEEGGILKF
                     GEAEELC"
     gene            2462..3226
                     /locus_tag="VN178_08315"
     CDS             2462..3226
                     /locus_tag="VN178_08315"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWS81014.1"
                     /translation="MAVDVGNTQTVLGLIDDGETRGQWRIATEAHRTADELAVFFAGL
                     LALEGLGPNEVSAMIVSSVVPGLTRSYRNLASGVFGVPYYGVTSEMETGLDNRYDDPE
                     QVGADRIVNAVGAGRHYGFPAIIVDIGTATTVEAVDGEACYLGGAILTGLYVSLDALV
                     SKTAKLPSVDLEEEPPRVIATNTPDSIRSGFIYGYAGAVDALIRRSRDELGEDGVRVI
                     ATGGPAGVIVPYCREIQEFDPDLTLKGLYALYEMNA"
     gene            3441..3929
                     /gene="greA"
                     /locus_tag="VN178_08320"
     CDS             3441..3929
                     /gene="greA"
                     /locus_tag="VN178_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWS81015.1"
                     /translation="MTDRNQELVTPEGLEKLQQELAYLTETRRKEVADRIRQAREFGD
                     ISENSEYDEAKNEQGLLERRIGELQRRVRNVKVVDPSEVGEDEVDLGTTVTLRVVGRE
                     GQERTFQIVGANESDPTSGKLSHASPVGRAVLKRKVGEKVMVSTPRGATEYEIVNVEA
                     AS"
     gene            3997..5472
                     /gene="lysS"
                     /locus_tag="VN178_08325"
     CDS             3997..5472
                     /gene="lysS"
                     /locus_tag="VN178_08325"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWS81016.1"
                     /translation="MSETGRSTGIHIPGWAERVADSLGEGPHVVVSGISVSGNIHAGN
                     LREVLVAEAVANALRQRGEEVRFIFHADTIDPLRKIAPGIPRSYEEYIGHSLSHIPDP
                     EGCHVSYAEHFLVPFEEALSEMEMDIEVLRSHELYERGLYTGVTGEALENTDELRWIL
                     QDVTNRKMPENWSPYLPRAESGKLTGNLVLSHVPEQTSVVFADEDGREDSADYSRGEG
                     KLGWRVELAARWKALGVTFEPFGKDHTSRGGSTDTADRMAREVFDYPVPGRYEYEWIG
                     LKGRGDMSSSRGIVLLPNDLLRIMPPGAVRRIILGRDPARRFDIDLEGGFPRFMDEYR
                     AEVGEPYVPFTHLVTVAQTVGEDTEAAAQMLRRGGYEEAVTDGLADDLYYARNWAAEW
                     APESMRLGLLDPSEAEEAAAALDDEQRAYLREVSEKLGPEMDGEAIQDVLYSTAIERG
                     LKPKRAFAAVYSVLLGKTSGPKAGPFIAGLTLDQVSQRFSV"
     gene            5794..8304
                     /locus_tag="VN178_08330"
     CDS             5794..8304
                     /locus_tag="VN178_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HWS81017.1"
                     /translation="MFERFTERARKVVVLAQDEARHFNHNYIGTEHLLLGLLREDEGV
                     AAQALYSLNVALDEVREQVESIVGYGEEGTGAQAPFTPRSKKVLELALREALQLGHNY
                     IGTEHILLGLVRESEGVAARVLSNLDVDPDKVRREVVRRLGGGRSRSRGGGEAGGGRG
                     VEAKRPKTRQLDQYGRNLTAYAEEDKLDPVIGRSQEIARIMQILVRRTKNNPVIIGEP
                     GVGKTAIVEGLAEEIAEGKVPEILGDKEVYTLDLGALVAGSKYRGEFEERLKKIMKEI
                     TDHGDIILFIDEIHNLVGAGAAEGAIDAASILKPALARGEIQVIGATTIDEYRKYVEK
                     DKALERRFQTIQVGEPSVEETELILKGLREKYEAHHNIDITDEALKAASQLGDRYIAD
                     RFLPDKAIDLVDEAASKMRIKTMSSPPYYKEIDEELAAVRSEKEAAIDGQEFEKAARF
                     RDSERKLVAQRRELEKAWREGKIEEKRVSIGENEIAEIVSMWTGIPVKKMTEEESARL
                     LQMEDALHGRIVGQNEAIKAVSRSIRRTFAGIKDPNRPSGSFVFLGPTGVGKTELART
                     LAEYLFGNQDAMIRLDMSEYMERHTVSRLVGSPPGYVGYDEGGQLTEQVRRRPYSVVL
                     FDEIEKAHPDVFNIMLQILEDGQLTDAQGRTVDFKNVVLIMTSNVGAQTINKTKSLGF
                     GSDDAGLSYKEMKGRVTSELRKIFRPELLNRIDEIIVFHKLEREDMRHIIEIQIKRLR
                     QQLIERDVTIEFTDEALNKLADEGYDPAFGARPLKRVLQRMIEDPMSEMILRGEVPNG
                     CKVIIEPNDKTAEEINEEESIVDIKVTQPKEIVKVESE"
     gene            complement(8386..8715)
                     /locus_tag="VN178_08335"
     CDS             complement(8386..8715)
                     /locus_tag="VN178_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81018.1"
                     /translation="MMTVLFYFERALRDLSSTEFTVVMLRENPGSPTFLLTLLGLLAF
                     WCGPVLTSRFVAEPVYGIFMSWEADAPPRDWREARARYFRANVVRGLGSAVAFVCFVV
                     AAITVPL"
     gene            complement(8712..9947)
                     /locus_tag="VN178_08340"
     CDS             complement(8712..9947)
                     /locus_tag="VN178_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS81019.1"
                     /translation="MPGATHSPREVRGNSSGRLVAVPGGHRDERPPNNLPLQLSSFVG
                     REKELAEVGRLLKETRLLTLTGSGGCGKTRLALAAAGELEGMFEDGVWLVELAPLADP
                     SLVPQAVASALGVRQQSGRPLSGSLSGYLRHKRALVVLDNCEHLIGACADLVDALLRS
                     CPGLRILGTSREALGITGEVAWPVPSLSLPDLRRLPEVASLPRYESARLFIERAAAVK
                     PSFALTERNAVAVAQVCYRLDGIPLAVELAAARTKMLSVEQIAGRLDDCFGLLTDGGR
                     TAMPRHQALHATMEWSHELLGREERVLFRRLSVFAGEFTLEAAESVCTGENLERDDVL
                     ELLSHLVDKSLVVVREEDGEARYRLLEKVRQYAREKLDGTGDEAEVGRRHLGFYVVLA
                     EEAEKELSGPDAPGSLRGS"
     gene            10263..11447
                     /locus_tag="VN178_08345"
     CDS             10263..11447
                     /locus_tag="VN178_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWS81020.1"
                     /translation="MRGVNLPLAGITVVSLEQAVAAPFATRQLADLGARVIKVERPGV
                     GDFARGYDETVMGMSSHFVWLNRSKESLALDLRQEEAKEILHRLVAGSDVFVQNLAPG
                     SAGRLGFGAVELREEHPRLVYCSISGYGEDGPYTKKKAYDLLVQCEAGLVSITGTPET
                     PSKVGISAADIAAGMYAYSGILSALFRRERTGEGATIEVSMLEALGEWMGFPAYFAGY
                     GGEEPRRSGAAHAAIAPYGPFECGDGEVVFLGIQNEREWKKFCEEVLGREGMAEDQRF
                     DKNSRRVENGDALHAEIEGVFSGLSSEEVIERLEGAGVANARMRTVRQFLDHPQLEAR
                     NRWREFGSPVGTLRGLLPPATLDGAEPVMAPIPEVGEHNEKLLAELGFGERDSATSGR
                     ET"
     gene            11518..>12340
                     /locus_tag="VN178_08350"
     CDS             11518..>12340
                     /locus_tag="VN178_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81021.1"
                     /translation="MSDTLIVLHTAISIIAIVLLILVVRVDPVISLVIGTIYLGLAAG
                     LGFEDTIGTIAQGFGDIMIEVGLLIGFGVLLGSLLLAMGALQKLVGLLLRLLGPQKLP
                     YAFSAALSTIFPAIYVDVQLVLAAPLARSAAPRMRGDGLAMMSGTLTAGILVGYVFVV
                     PGLGTVAIAGLLNVPLGTMLIFGTLIGLPTAVLTTFIYGRLLRYGLWNKAKDEVGFQE
                     AEEVSPAETEQQKQQQTENTPPLYVSLLPILVALLLIAFGAIAEAAGLESPVVTFF"
BASE COUNT         2457 a         3955 c         3953 g         1975 t
ORIGIN      
        1 gacgcgctcg gccgcgcccg cggcgccttc ccgggtgctg gcggccacga cgagcgcgag
       61 cagaccctga accagctcct cgtcgagatg gacggcttcg actcgaagag cgggatcatc
      121 atgctcgccg ccaccaaccg cgcggacatc ctcgaccccg cgctcctcag gccgggccgt
      181 ttcgaccgcc agatcgtggt cgacaggccg gacctccccg ggagggagaa gatcctcagg
      241 gtgcacaccc gcggcaagcc ccttggcccg gacgtagagg tcggcacgct cgccaggggg
      301 acccctggct tcaccggcgc cgatttggcc aacctcgtca acgaggccgc gctccttgcg
      361 gcgcgtcacg acaagaacca gatcgacatg tccgagatgg aagaggccat agaccgcgtg
      421 atcgccggtc ccgagcggaa gacgcgcctt atctccgaca aggagaagga gatcaccgcc
      481 taccacgagg gcgggcacgc catcgtcggc gcgctccttc ccgatgccga cccggtgcac
      541 aagatcacca tcatccctag aggccaggcg ctgggcgtaa ccatgagcct gcctacggaa
      601 gaccgcttta tgatgagccg cggccagctc atggcgcagc tcgccatgat gctcggcggg
      661 cgggccgccg aaagggtggt cttcgatgag atcaccactg gcgcctccaa cgacctcgaa
      721 cgggtaaccc agaccgccag gcagatggtc acccgcttcg ggatgagcga gaagctcggg
      781 cccatggcgc taggccacca gcaggggcag gtctttatgg gacgcgactt caacaaccag
      841 ccggattact cggacgagat cgccttccag atagacaagg agatccgcag gatcgtcgac
      901 gagtcctacg acacggccga ggacgtactg gttcgtaaca gggagctgct ggacaagctc
      961 tcggccgagc tgatcgagta cgagactgta gacgccaatc acctgaagcg cctgatcgag
     1021 gagtacgccg tggatagcat ctcaccgaac ggtgccgtcc ccgactccga cggccgctac
     1081 aggggtcgct accaggacta gatgggccgc acggcccgga acacagacgg ggccgggacc
     1141 ctaacgacgg gtcccggccc cgtcgtcgtc ggcatcctga acgtcacccc cgactccttc
     1201 tccgacggtg gggacttcct cgacccggaa gccgctgccc ggcgcgccgc ttccatgctc
     1261 gacgaagggg ccggcatgct ggacgtcggt ggagagtcca cgcgccccgg ctcggaccct
     1321 gtctcgcagg aggaagagat gaggcgcgta atccccgtta tcggggggat cctcgccgcg
     1381 cgtcccgaag cggtgatctc ggtcgacacc tatcggtcag gcaccgccgc ggcagccctc
     1441 gaagccggcg cacgcctcgt caacgacgtc accgcgctcc gcggcgaccc cagcatggcc
     1501 tccgtggtag gggaggccgg atgcccggtt atcctgatgc acatgcaagg cgagccgaag
     1561 accatgcaga aggagcccca ctacgaggat gtggtgcgcg aggtcaggga tttcctcgct
     1621 gagagggccg ggtacgccgt cgatgcaggc gtccgacccg agaacataat cgtcgatcca
     1681 ggcatcggct tcgggaagaa cctccagcat aatctggatc tcctcaacaa cctggacgcg
     1741 atcgtagatc tgggtttccc agtcctgatc ggggcctcgc gcaagcgctt tatagagggg
     1801 attacgggcg tgcaagaggc gagaaaccgc gtccccggca cggtcgccac aacggtgctc
     1861 gcctacgaac ggggcgcgac cttcttccgg gtgcacgacg tgcgcgccaa ccgcgaagcg
     1921 ctcgccgtcg ccgaagccgt gctcgacacc tcccgccggt gagcaaggtc ttcctgagcc
     1981 tcggcagcaa cctgggcgac cgtctggccc acctcagggc cgtggtcgcc gccctcggtc
     2041 ggggtcctcg catgagggtc gcgggtatat cgaaggtcta cgagacggta ccggtcgagg
     2101 tcgaagaaga gcagccagac tacctgaatt gcgtggtcga actggagtgt ggtgtccccg
     2161 ccatcgagtt gcttcgctat tgccagggga tcgaggccgc gctcggcagg gagcgcgccg
     2221 aagccggcga gaaggcccca cgcaccgcgg atatagacgt actgctgttc ggagaggaga
     2281 ccattgtgga acccgatctt cgggtgccgc acacgggggt gacgcgtgcc ttcaatctca
     2341 ggggcctcgc ggacctggat gccaatctgt atattccaca gcgcggaacg gtcggtgaac
     2401 tgctagaaag ttcggaagaa ggaggcatcc tgaagttcgg ggaggctgaa gagctttgtt
     2461 gatggccgtg gacgtgggca atacgcagac cgtcctcggg ctcatcgatg acggggagac
     2521 gcggggacag tggcgtatcg ccacggaggc tcataggacg gccgacgagc tcgccgtctt
     2581 cttcgcaggc cttctcgcgc tcgaaggtct tggccccaac gaggtgagcg ccatgatcgt
     2641 ctccagcgtc gtgcccggtc tcacccgttc ctaccgcaac ctcgcctccg gagtcttcgg
     2701 ggtaccctac tacggggtga cctcggagat ggagacaggc ctcgacaacc gctacgacga
     2761 ccctgagcag gttggggcag acaggatcgt caacgctgtg ggggcaggta ggcactacgg
     2821 gttccccgcg atcatcgtcg atatagggac ggcgaccacc gtcgaggctg tggacggcga
     2881 agcatgctac ctgggaggcg ctatactgac gggcctctac gtctccctgg atgcgctcgt
     2941 ctccaaaacg gccaagctcc ccagcgttga cctcgaagaa gaaccaccca gggtcatagc
     3001 caccaatacc ccagactcca tccgcagcgg tttcatctac ggctacgccg gcgccgtcga
     3061 cgccctgatc cgaaggtccc gtgacgagct gggcgaggac ggcgtcaggg taatcgccac
     3121 aggaggtccc gccggggtca tcgtgcccta ctgccgtgag atacaggagt tcgaccccga
     3181 cctcaccctg aagggcctct acgccctcta cgagatgaac gcgtgaatag cacttcagca
     3241 cgccgccagg cttcgccagg cggcttgtca gcatttcagc tttcgcctgc gcaacccctg
     3301 gctgacatgc tgaaaggcgg cggagccgcc gtgctgacaa gtggaggccg aaggccggag
     3361 cgtgctgaca ggctgaaacg ctgcccgcgg gcagcgtctc attgacaccc ccggcacccg
     3421 cccttataat ctcgcggctt atgaccgata ggaaccagga gctcgtcacc cccgaaggtc
     3481 tcgagaagct ccagcaggaa ctcgcatacc ttaccgagac gcgccgcaaa gaggtcgccg
     3541 accgcatccg ccaggcccgt gaattcgggg acatctccga gaattccgag tacgacgagg
     3601 cgaagaacga acagggcttg ttggagcgcc ggatcggtga gctccagcgc agggtacgca
     3661 acgtgaaggt ggtcgaccct tcggaagtcg gtgaagacga ggtagatctg gggaccacgg
     3721 tgaccctcag ggtcgtcggc agggagggac aggagcggac gttccagatc gtcggtgcca
     3781 acgagtccga cccgacgagc gggaagctca gccacgcctc cccggtgggc cgggccgtgc
     3841 tcaagaggaa ggtgggggag aaggttatgg tctctacccc ccgcggcgcg acggagtacg
     3901 agatcgtcaa cgtcgaggcg gccagctagg gcgccgggag cgcacgacgc cccgcttctc
     3961 caaagttccg accaccgaac cctattggag taccgcatga gcgagaccgg taggtcgacc
     4021 ggcattcaca tacccgggtg ggccgagagg gtcgccgatt ctcttggcga ggggccgcac
     4081 gtggtcgtga gcggcatcag cgtctcgggc aacatccacg ccggcaatct gcgcgaggtc
     4141 ctcgtcgcag aggcggtcgc gaacgctctg cggcaacgcg gtgaggaggt ccgtttcatc
     4201 tttcacgcgg acacgatcga cccgctgcgc aagatagccc ctggcatccc caggagctac
     4261 gaggaataca tcggccacag cctctcgcac atacctgacc ccgaaggctg ccacgtctcc
     4321 tacgccgagc attttctcgt gcccttcgaa gaggcgctct ccgagatgga gatggatata
     4381 gaggtgctcc gctcgcacga gctgtacgag cggggcctct acaccggggt taccggcgag
     4441 gccctcgaga acacggacga gttgcgctgg atccttcagg acgtcacgaa tcgaaagatg
     4501 cccgagaact ggtcgcctta tttgccccgt gcggaatcgg ggaagcttac cgggaacctg
     4561 gtgctctccc atgtgccgga gcagaccagc gtcgtcttcg cggatgagga cggccgggag
     4621 gacagcgcgg attactccag aggcgagggc aagctgggtt ggcgggtcga gctcgcggcc
     4681 aggtggaagg ccctcggggt taccttcgag ccttttggta aagaccacac cagtcgcggc
     4741 ggctcgacag acaccgcaga ccgcatggca agggaggtct tcgactaccc cgtccctggc
     4801 cgctacgagt acgagtggat agggctcaag ggtcgtgggg acatgagcag ctcgcggggt
     4861 atcgtgctac tgccgaacga tctcctgagg ataatgccac ccggcgccgt gcggcgcatc
     4921 atcctcggcc gcgaccccgc ccgacgcttc gatatcgacc tggagggcgg cttcccgcgc
     4981 tttatggacg agtaccgcgc agaagtagga gaaccctacg tccccttcac ccatctggtc
     5041 accgtcgccc agaccgtcgg cgaagacacg gaggccgccg cgcagatgct gcggcgtggc
     5101 gggtacgagg aggcggtcac cgacgggttg gcggatgacc tctactacgc ccgcaactgg
     5161 gccgcggagt gggcccccga gtcgatgcgg ctgggattgc tcgacccgtc cgaggcggag
     5221 gaggctgccg cggcgctcga cgacgagcag cgcgcgtacc tgcgtgaagt ctcggagaag
     5281 ctcgggccag agatggatgg agaggccatc caggacgtgc tgtactccac cgccatcgag
     5341 cgcgggctca agcccaagag ggcgttcgcc gccgtctaca gtgtgttact aggcaagacc
     5401 agcgggccca aagccggccc tttcatcgcc gggttgaccc tggatcaggt cagccagagg
     5461 ttctcggtgt aagacgagac accacatcgg ctggaaggcg acgatagact gttgtatggc
     5521 gcaacaggag gtcgatccgg tccaccgcga cacgatgtat agcgcttaga gcgtaaagct
     5581 ccaggctggt accgaaatac aggcccaacg gttgtatact tcaggttagc cggcacgagc
     5641 tagagaggag ctcaagagag aggcgcacgt tatttcgaaa ggcagatttt tagagatgtg
     5701 taatccgtcc atgttcgata ctcgtataca gatacagacc gttatgatcc cggatgccgt
     5761 gaaggaccct cgaagcagag gagattcccg ctaatgttcg aacgtttcac ggagcgggcc
     5821 cgcaaagtcg tggtgctcgc acaggatgag gcccgtcatt tcaaccacaa ctacatcggc
     5881 accgagcacc tgctcctcgg tctccttcgt gaggacgagg gcgtcgctgc ccaggcgctc
     5941 tactccctga acgtggccct cgacgaggtg cgcgaacagg tcgagagcat cgtcggctac
     6001 ggcgaggaag gcacgggcgc gcaggcgccg tttacgccac gctccaagaa ggttctggag
     6061 ctcgcgctca gggaggcgct gcagctaggc cacaactaca taggcaccga gcacatcctt
     6121 ctcggcctgg tcagggagag cgagggcgtc gcggccaggg ttctctccaa cctcgacgtc
     6181 gaccccgaca aggtgaggcg ggaggtcgta aggaggctcg gcggagggcg cagccgcagc
     6241 cggggcggtg gcgaggccgg cggaggacgc ggggtggagg ccaagcggcc caagacccgc
     6301 cagctcgacc agtacggcag gaacctcacg gcctacgccg aggaggacaa gctcgacccc
     6361 gtcatcggca ggagccagga gatagcgagg atcatgcaga tcctcgtcag gcgtaccaag
     6421 aacaaccccg tcatcatcgg tgagcccggc gtcggcaaga ccgccatcgt cgaggggctg
     6481 gccgaggaga tcgcagaggg taaggtcccg gagatccttg gggacaaaga ggtctacacg
     6541 ctcgacctcg gggcgctggt cgccggctcc aagtaccgcg gtgagttcga ggaacgcctc
     6601 aagaagataa tgaaggagat aacggaccac ggcgacatca tcctgtttat cgatgagatc
     6661 cacaatctcg tcggagccgg ggccgccgag ggcgccatcg acgccgcctc catcctcaag
     6721 cccgcgctgg cgcgcggtga gatccaggta atcggcgcga cgacgataga cgagtaccgc
     6781 aagtacgtgg agaaggacaa ggccctggag cgccgcttcc agaccatcca ggtcggcgag
     6841 ccttcggtcg aggagaccga gctcatcctc aagggcctgc gcgagaagta cgaggcgcac
     6901 cacaacatag acatcaccga cgaggcgctt aaggccgcct cgcaactcgg cgaccgctac
     6961 atcgcggacc gcttcctgcc cgacaaggcc atcgacctcg tcgacgaggc cgcgtccaag
     7021 atgaggataa agaccatgtc ctcgccgccc tactacaagg agatagacga agaactcgcc
     7081 gccgtgcgct ccgagaagga ggccgccatc gacggccagg agttcgagaa ggccgcgcgc
     7141 ttcagggact ccgagcgcaa gctcgtcgcc cagcgccgcg aactcgagaa ggcctggcgg
     7201 gagggcaaga tcgaggagaa gcgcgtctcc atcggcgaaa acgagatcgc cgagatcgtc
     7261 tccatgtgga ccggcatccc ggtcaagaag atgaccgaag aagagtcagc tcgcctcctc
     7321 cagatggagg acgccctgca cgggcgcatc gtcgggcaga acgaggccat aaaagctgtt
     7381 tctcgttcta tccgccgtac cttcgcgggt ataaaggacc cgaaccgtcc ttcagggtcg
     7441 ttcgtcttcc tcggtcctac cggagtgggc aagaccgagc tcgcgcgtac tctcgccgag
     7501 tacctcttcg gcaaccagga cgccatgatc cggctcgaca tgtccgagta catggagcgc
     7561 cacacggtga gccgcctcgt cggctcgccc cccggctacg tcggctacga cgagggcggg
     7621 cagctcaccg agcaggtgcg gcgtcgtccc tactcggtcg tcctcttcga cgagatcgag
     7681 aaggcccacc ccgacgtctt caacatcatg ttgcagatac tggaggacgg gcagctcacg
     7741 gacgcgcagg gccgcaccgt ggacttcaag aacgtcgtcc tcatcatgac ctccaacgtc
     7801 ggggcgcaga ccataaacaa gaccaagtcg ctcggcttcg gctccgatga cgccggtctc
     7861 tcctacaagg agatgaaggg ccgcgtcacg agcgagctgc gcaagatctt ccgtcccgag
     7921 cttctcaaca ggatcgatga gatcatcgtc ttccacaagc tcgagcgcga ggacatgcgc
     7981 cacatcatcg agatccagat aaagcgcctc cgccagcagc tcatcgagcg tgacgtgacg
     8041 atcgagttca ccgacgaggc cctgaacaag ctcgccgatg agggctacga cccggccttc
     8101 ggcgcgcgcc ccctgaagcg tgtgctgcag cgcatgatcg aagaccccat gagcgagatg
     8161 atcctgcgcg gcgaggtgcc caacggctgc aaggtcataa tagagcccaa cgacaagacc
     8221 gcagaagaga tcaacgagga agagtccata gtcgacatca aggtcaccca gccaaaggag
     8281 atcgtcaagg tcgaatctga gtagggaaaa ccaaggatct acatcgaggc cggggcattt
     8341 tcacccccgg cctcttcatt gttccatctg tggacgttcg acgagttata gcggcaccgt
     8401 gatggccgcg acaacgaagc agacgaaggc caccgcagat cccagcccgc ggaccacgtt
     8461 ggcgcggaag tacctcgcgc gtgcctcccg ccagtctcgc ggtggcgcat cggcctccca
     8521 gctcatgaag atcccgtaga ccggctcggc gacgaagcgc gaggtgagga cggggccaca
     8581 ccagaacgcg agcaggccca agagcgtcag caaaaaggtg ggactacccg gattctcgcg
     8641 cagcatcacc acggtgaatt cggtgctcga caggtccctt agcgcccttt cgaagtagaa
     8701 caacacggtc atcatgagcc ccgtaaagag cccggtgcat ccggcccgct aagctccttc
     8761 tcggcctcct ccgcaagcac cacgtagaag cccaagtgcc gtcgtccgac ctcggcctcg
     8821 tccccggttc cgtctagctt ctccctggcg tactgccgaa ccttctccag cagtcggtag
     8881 cgggcctccc catcctcttc ccgcaccacc accagcgatt tgtccaccag gtgcgagagc
     8941 aactccagca cgtcatcgcg ttcgagattc tctcccgtgc ataccgactc cgcagcctcc
     9001 aacgtgaact cgccagcgaa caccgaaagg cggcggaaca agacccgctc ctcccgacca
     9061 agcagttcgt ggctccattc catcgtcgcg tgcagcgcct ggtggcgggg catcgctgtc
     9121 ctgccgccgt ccgtaagcag cccgaagcag tcgtccaggc gcccggcgat ctgctccacc
     9181 gacaacatct tcgtcctcgc cgccgccagc tccacggcga gcgggatgcc gtccagtcgg
     9241 tagcaaacct gggccacggc taccgcgttc cgctccgtca gagcgaacga cggcttgacc
     9301 gccgccgccc tctcgatgaa gaggcgggcc gattcgtacc ggggcaagct cgcgacctcc
     9361 ggcaggcgcc gcaggtcggg cagggagagc gaggggaccg gccaggcgac ctcgccggtt
     9421 atcccaaggg cctcccggct cgtcccaagg atgcgcagcc ccgggcacga acgcagcagc
     9481 gcgtcgacga ggtcggcgca ggcgccgatg agatgctcgc agttatccaa gaccaccagc
     9541 gccctcttgt ggcgcaggta ccccgagagg gacccgctca gcgggcgtcc cgactgctgg
     9601 cgcacgccca gagcggaggc cacagcctgg ggcacgagag aagggtccgc caacggcgcc
     9661 agctccacca gccacacacc atcctcgaac atcccttcca gctctcccgc ggccgccaaa
     9721 gccagcctcg tcttgccgca cccgcccgag ccggtgagcg tcagcaggcg ggtctccttc
     9781 agcagccgcc ctacctcggc cagctccttc tcacgtccga cgaagctaga gagctgaaga
     9841 ggcaggttgt tgggcgggcg ctcatcccga tgcccgccgg gtacggcgac cagccggccg
     9901 ctactgttcc ctcgtacttc acggggggaa tgcgtcgctc cgggcattgg atttgctcct
     9961 aaagttactt cgtgctggtt ggggaagtgt agccgctagg cggcccaact tgaagagtat
    10021 tcccttcgat cgctgcaaag ctgtgcgtct ccggcaagtt cggcgcactc acccctcgat
    10081 actcgacacg tcttgatctg attcagggaa taaaaaacag aaaggaggga ataggagggt
    10141 tagtaggccg caaccgaaac cccgcgtccg accgcagcgg atcgcgaagc gcggctggga
    10201 gagactatga acgtcgaagc agtcgaggtg gaatacgtgc ccggtcctga cgagtacata
    10261 gggtgagggg tgtgaatctc cctctagcag ggataaccgt cgtctccctg gagcaggcgg
    10321 tcgcggcccc cttcgccacc cgccagctag cggatctcgg cgcgagggtc ataaaagtcg
    10381 agcgccccgg cgtcggggac ttcgcccggg gctacgatga aacggtcatg ggcatgtcca
    10441 gccacttcgt ctggctcaac cgctccaaag agtcattagc gctagatctt cggcaagagg
    10501 aggccaaaga gatcctgcac cggctcgtgg ccgggtccga cgtcttcgtc cagaacctgg
    10561 cccccggctc ggccggacgc ctcggcttcg gcgccgtaga gttgagggaa gagcacccgc
    10621 ggctggtgta ctgctccatc tcgggctacg gcgaagatgg gccttacacg aagaagaagg
    10681 cctacgacct gctcgtgcag tgcgaggctg gactggtctc cataacgggt actcctgaaa
    10741 cgccatccaa ggtcggcatc tccgccgccg acatcgccgc agggatgtac gcctactccg
    10801 gcatcctctc cgccctcttc agacgcgaga ggacgggcga gggagcgacc atcgaggtct
    10861 cgatgctcga agccctagga gagtggatgg gcttcccggc gtacttcgcc gggtacggtg
    10921 gtgaggagcc ccggcgcagc ggcgccgcgc acgcggcgat agcgccctac gggccgttcg
    10981 agtgcgggga tggcgaagtc gtcttcctcg ggatacagaa cgagcgcgag tggaagaagt
    11041 tctgtgagga ggttctgggg cgcgagggga tggcggaaga ccaacgcttc gacaagaact
    11101 ccaggcgcgt cgagaacggc gacgcgctgc acgccgagat tgaaggtgtg ttctccggcc
    11161 tctcatctga agaagtcatc gagaggctcg aaggggcggg ggtggcgaac gccaggatgc
    11221 gcaccgtgcg ccaattcctg gatcaccccc agcttgaggc ccgaaaccgc tggcgcgagt
    11281 tcggctcgcc ggtgggtacg ttgcgtgggc tcctccctcc ggccaccctg gatggcgcag
    11341 agccggtcat ggcccccatt cccgaggtcg gggagcacaa cgaaaagctc ctcgcagagc
    11401 ttggctttgg ggaaagggac agcgcgacgt ctggcagaga gacgtaacga tccgccgact
    11461 accacccaga aagaagttcg gctcgtggcg ccgaccagag cctggaagga gcgtagcatg
    11521 agcgacacct tgatcgtgct gcacacggcg atctctataa tcgcgatagt gttgctgatc
    11581 ctggtcgttc gcgtcgaccc ggtgatctcc ctggtgatag gcaccatcta cctgggtctg
    11641 gcagcgggcc ttggcttcga ggatacgata ggaaccatag cgcaaggctt cggcgacata
    11701 atgatcgaag taggcctgct catcggcttt ggcgtcctct tgggctcgct gctgctcgcc
    11761 atgggagcgc tccagaaact ggtcgggctg ctgctgagac ttctcggtcc ccagaagctc
    11821 ccttatgctt tcagtgccgc cttgagtacc attttcccgg ccatctacgt ggacgtccaa
    11881 ctcgtgctcg cggcccccct ggcacggtcg gccgccccca ggatgagagg ggatgggctt
    11941 gcgatgatga gcgggacgct gaccgccggc atactggtgg gatacgtctt cgtcgtcccg
    12001 ggtctgggaa ccgtggcgat agccgggttg ctgaacgtgc cgctgggcac gatgttgatc
    12061 ttcggaacct tgatcggcct ccctaccgcc gtactcacca cgttcatcta cggccggctc
    12121 ctgagatacg ggctatggaa caaggccaag gatgaagtgg gtttccagga ggcggaggaa
    12181 gtaagccccg ccgaaacgga acaacaaaaa caacagcaga cggagaatac tcctcctctg
    12241 tacgtctcgc tgttgcctat cctggtcgcg ttattgctga tcgccttcgg ggcgatcgcc
    12301 gaggccgccg gtctagagtc cccggtagta acgttcttcg
//
LOCUS       DATMVF010000168         6299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_661250, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000168 DATMVF010000000
VERSION     DATMVF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661250"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VN178_08355"
     CDS             complement(<1..897)
                     /locus_tag="VN178_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS81022.1"
                     /translation="MLDRPEPDVRQLHAALDGKLEGYLRTDEASRAMWSTDASIYLRK
                     PVGVVVARTEVDVELTLSAARDLGLSITPRGTGTSLAGQATAPGLALDVSEMRRALEV
                     DLEGRRCVVEPGLVQGELNAMVEPHGLVFGADTSTSDVATLGGMVGNNSAGMRSLVYG
                     MTSDQVLGLRCVLASGETVEIGPLGRDEARGRARHADALARLLRGAMEIGDRYGDEIK
                     RRFPKMIRRVSGYGLDALVVPETLDLTRLICGSEGTLAIVTRAEFRLHPLPEACALAS
                     FEFDSLSAAARATNRFLRMNPSA"
     gene            complement(938..1780)
                     /locus_tag="VN178_08360"
     CDS             complement(938..1780)
                     /locus_tag="VN178_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWS81023.1"
                     /translation="MRVALAQIDCVLGDTEPNLRKAKEVVAEARDRGADLTVFPELSL
                     SGYALGELGDDVAIEAQSEPIISLAEVAGEMGVVVGFCEEGRGFQTYNSAAYLEGGVL
                     GHLHRKLFLPNYRIYEERKLYNPGQNMRAFDTRFGRMAILICNDAWQTFLPFIAVQDG
                     AQVLIIPAASGPYPYPEMLDTRGYWRGITRFYARMLESYVVFVNRVGEENSLTFWGYS
                     HVVDPWGTVVAEAPVHEEALITVDIDLGNVRRRRREVPLVKEARLALLARELNRLAEE
                     GGDL"
     gene            complement(1905..2861)
                     /locus_tag="VN178_08365"
     CDS             complement(1905..2861)
                     /locus_tag="VN178_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWS81024.1"
                     /translation="MRTTRAKLTQLLGLFAVLFLIVAGCGGAGGGGASGDVDLSGAEF
                     TVGSKEFTEQLILGQITLQVLENAGATVNDQIGLAGTVAARKALESGEIDMYWEYTGT
                     GWITHLGHTDPIPNRQKQFEAVAKEDLKKNDIKWLSPPSPANNTYTMAVRSEAYDKLG
                     VKKLSDFKQLVEENPEEATVCVGTEFSTRDDGLPGMEKAYDFEFPKQNIVKIDEGLIY
                     QQTDKGKQCNFGEVFQTDGRIAALDLELIEDDKSFFPIYNPALNVRKDVIEEYPQIAE
                     LFAPISEKLTTQELQALNAKVDVEGQLEDQVAEEWLSENGFI"
     gene            complement(2914..3678)
                     /locus_tag="VN178_08370"
     CDS             complement(2914..3678)
                     /locus_tag="VN178_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS81025.1"
                     /translation="MTTLQKAAAKGPGGAARLLRYFTMPIFLGLAGLALYLYVGTQDL
                     DSIEQRSLNAGTLIQQTARLLELAAVSTAIVVTLAVTTGVLLTRPFARRVSPFVIGLA
                     NIGQAVPSIGVLALLAIIWSIGFWPCIVALVAYSFLSILRNTMVGLRQVDRSIIEAAR
                     GMGMTKLAVLFRIELPLAVPIILAGIRTALIINVGTATLGTFIGAGGLGESINNAIRL
                     DRTVVLVAASGVTAIVALFVDWLAGVAEDVLRPRGL"
     gene            complement(3675..4919)
                     /locus_tag="VN178_08375"
     CDS             complement(3675..4919)
                     /locus_tag="VN178_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HWS81026.1"
                     /translation="MTDRAAEENNTQTAETMIRLEDLTKVFPAQEEAAVDDLSLEIYE
                     GEIVVLVGPSGCGKTTTMKMINRIIEPTSGRIFLQGEDVTKVNPDKLRRRIGYVIQQI
                     GLFPHMTIADNIATVPRMLGWEKKRTSDRIDELLETVGIDLSYRDRYPKELSGGQRQR
                     IGVARAMAADPPVLLMDEPFGAIDPITRDRLQDEFLRLQQEIRKTIVFVTHDIDEAIK
                     MGDRIAILKHQSKIAQYDTPERILTDPADEFVQDFIGSGPSIKRLSLSRVSDIETSDW
                     PVARLTDSHDEVREKMRSTSRDHVLLLDEQEKPGRWVGLEEIQHDDTPLEESGRPAVA
                     IVEANTNLYDTLDTMITSYKGSAIIVDEGGRYAGVVDFDTVLEAINTMRPHERDRHMS
                     PAEGEQPRERTGQDLQAGGEER"
     gene            complement(4912..5562)
                     /locus_tag="VN178_08380"
     CDS             complement(4912..5562)
                     /locus_tag="VN178_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS81027.1"
                     /translation="MSSFLDYISLSWDHVLELAIGHAIVVAIALVIDIVIGVALGVLV
                     YRRESAANVALAITSTFLTIPSFALFGLFIPIFGLGYLPAVVALVLYGLLPILRNTVA
                     GLRSVDPAIAESAQGMGMNARERLLRIELPLAWPVIITGVRVSTMLLMGIAAIAAVVN
                     GPGLGDDIFAGLARIGSPTALNLVIGGILGIIVLALLFDTFFNLLGKLTTSRGIRD"
     gene            complement(5672..>6299)
                     /locus_tag="VN178_08385"
     CDS             complement(5672..>6299)
                     /locus_tag="VN178_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWS81028.1"
                     /translation="DTETLQYRLGWRFFALAARASEQRLLSVTPTLLERLVENVGETA
                     HLSVLQGVEVLTVLSKSPTHAVKADGWVGRMVHIYCTSSGRALLFDHDSEALSSLLSG
                     VEFRRLRPGTVRNIEELEERIAVARERGYALVDEEFEFGLVGAAAPVRDFKGKLVAAL
                     NISAPKFRLGGRLEAAGREVKRAADDLSTLLGWSADPRPATDDASGVS"
BASE COUNT         1147 a         1980 c         1945 g         1227 t
ORIGIN      
        1 cgccgatggg ttcatccgca ggaaccggtt tgtagcgcgg gccgctgcgg agagggaatc
       61 gaactcgaaa gaggccaggg cgcaggcctc ggggagcggg tgtagtcgaa actcggcgcg
      121 ggtgacgatg gccagcgtgc cctcggagcc gcagatcagg cgcgtcaggt ccagggtctc
      181 gggaacgacg agggcgtcga ggccgtagcc ggagacgcga cggatcatct tcggaaaccg
      241 ccgctttatc tcgtccccgt aacggtcccc gatctccatc gccccgcgca gtagcctcgc
      301 gagcgcgtcg gcgtgacggg cgcgccccct ggcttcatcc ctgccgagcg ggccgatttc
      361 cacggtctcc ccggaggcga gcacgcagcg cagtccgagg acctgatccg aggtcatccc
      421 gtagacgagc gagcgcatcc ccgcggagtt gttcccgacc atgcccccta gcgtcgccac
      481 gtcagaagtg gatgtatcgg ccccgaacac gagcccgtgc ggctcgacca tcgcgttgag
      541 ctcgccctgc accaggccag gctccacgac gcaacgccgc ccttcgaggt ctacctccag
      601 cgcccggcgc atctcggaga cgtcgagggc cagccctggc gccgtcgcct gcccggccag
      661 ggaggtgcca gtgccgcggg gcgtaatcga gagcccgagg tcgcgggctg ccgagagagt
      721 tagctccacg tccacttccg tgcgggcaac gacgacgcca acgggcttgc ggaggtagat
      781 ggaggcgtcc gtggaccaca tagcgcgtga ggcttcatcg gtgcgcaggt acccttcgag
      841 cttgccgtcg agggcggcgt ggagttgcct cacgtcgggt tcgggtctat ccagcatgct
      901 ctggtcaact ttacatccag agcgggcgtg ttgccaatca gaggtccccg ccttcttcgg
      961 cgagacggtt caattccctg gccagaaggg cgaggcgggc ctcctttacc aggggcactt
     1021 ccctgcggcg ccggcgaacg ttaccgaggt ctatgtctac ggttatcagg gcctcctcgt
     1081 ggaccggcgc ttcggcaact accgtccccc agggatcgac gacgtgcgag tagccccaga
     1141 aggtcaggct gttctcctct ccgacccggt tgacgaagac gacgtagctc tcgagcatgc
     1201 gggcgtaaaa gcgggtgata ccacgccagt agcccctcgt gtccaacatc tcaggatagg
     1261 ggtaggggcc gctggcagca gggataatga gcacctgggc gccgtcctgg accgcgatga
     1321 aaggtagaaa cgtttgccag gcgtcgttgc agattaggat ggccatgcgg ccgaacctgg
     1381 tgtcgaaggc acgcatgttc tgtcccgggt tgtagagctt tctctcctcg tagatcctgt
     1441 agttcggcaa gaagagcttg cggtgcaggt gcccgagaac tcctccttcg agataggccg
     1501 cactgttgta ggtctgaaag ccccgcccct cctcgcaaaa ccccacgaca acacccatct
     1561 ctcctgccac ctcggccaga gagatgatag gctcgctttg cgcctcgatg gcgacatcgt
     1621 cgccgagctc tccgagggcg tagccggata gactcagctc agggaagacg gtgaggtcag
     1681 cgcccctgtc tctcgcctca gccacgacct ctttcgcctt gcggaggttc ggttcggtgt
     1741 cgccgaggac gcagtcgatc tgggccaatg ctacccgcac ggttgcttgc tacccctttc
     1801 tttggtgcgt tcctcccgca cgtaatctcg gagtagggcc gccgggaaca gggatagaag
     1861 cgattgccgg atgttgcacc cggcaatcgc catcggatag ctccttagat gaacccgttc
     1921 tcgctgagcc actcttcggc cacctggtct tcgagttgcc cttccacgtc gaccttcgcg
     1981 ttcagagcct gcagctcctg ggtggtgagc ttctcggaga tgggcgcgaa cagctctgca
     2041 atctgcgggt attcctcgat cacatccttg cgcacgttga gcgccgggtt gtagatcggg
     2101 aaaaagctct tgtcgtcctc gatgagctcg aggtcgagcg ccgcgatgcg accgtcggtc
     2161 tggaacacct caccgaagtt gcactgcttg cccttgtcgg tttgttggta gatcagacct
     2221 tcgtctatct tgacgatgtt ctgcttcggg aactcgaaat cgtatgcttt ctccatcccc
     2281 ggcaggccgt cgtcgcgggt cgagaattct gttcccacac acacggtggc ctcttcagga
     2341 ttctcctcga ccagctgctt gaagtccgaa agcttcttga cgccgagctt gtcgtaggct
     2401 tcgctgcgca cggccatagt gtaggtgttg ttggccggag agggcggaga gagccacttg
     2461 atatcgttct tcttgaggtc ctccttggct acggcttcga attgcttctg acgattcggg
     2521 atgggatccg tgtggccaag gtgggtgatc caccctgtcc ccgtgtactc ccagtacatg
     2581 tcgatctcgc cggattccag cgccttgcgg gctgcgaccg ttccggccaa gccgatctgg
     2641 tcgtttaccg tcgcccctgc attttcgaga acttgcagcg tgatctggcc taggatcaac
     2701 tgctcggtga actccttgga gccgacggtg aactcggcgc cggagaggtc tacgtccccc
     2761 gatgctcctc cgccaccggc gccgccgcaa cccgcgacaa tcaggaacag aactgcgaag
     2821 agcccaagca gttgtgtcag ctttgcacgt gtcgttcgca caacgatcct cctttatggt
     2881 cttgtgtggt cgagagcttt acgatggttc cggctacagg cccctggggc ggagaacatc
     2941 ttcggccacg ccagcgagcc agtcgacgaa cagggccacg atggcggtca ccccgctggc
     3001 ggcgaccagc acgaccgtcc ggtccagcct tatggcgttg ttgatcgact cccccaatcc
     3061 gccggcacct atgaacgtcc caagcgtcgc ggtaccgacg ttgatgatca gggcggtcct
     3121 gatgcctgcc aggattatgg ggacggcgag tggcaactcg atgcggaaga gcacagccaa
     3181 cttcgtcatt cccatgccgc gggcggcctc gataatggag cgatccacct gccgcaagcc
     3241 caccatggtg ttccgcagga tagacagaaa cgagtacgcc acaagcgcga cgatgcaagg
     3301 ccagaacccg atactccaga tgatggccag taacgccagc acaccgatgg acgggaccgc
     3361 ctggcctatg ttggccagcc cgatcacaaa aggggacacc cggcgggcga agggtcgggt
     3421 gagcagaacg ccggtggtca cggccaacgt aacgacgatc gcggtagaga cggcggcgag
     3481 ctcgaggagt ctggccgtct gctggatgag ggtgcccgca ttcagggagc gctgctcgat
     3541 gctgtcgaga tcctgcgtac ctacgtacag gtagagtgcc aggccagcga gcccgaggaa
     3601 gatgggcata gtgaagtagc gtaataggcg cgccgcgccg cctggacctt tcgcggcggc
     3661 cttctgtaag gtcgtcatcg ctcttcacct ccggcttgca gatcctgccc agtcctctca
     3721 cgcggctgtt ccccttccgc ggggctcatg tggcggtccc gctcatgcgg gcgcatggtg
     3781 ttgatcgcct caagcaccgt atcgaagtcg accacgcccg cgtagcgccc cccctcgtcg
     3841 acgatgatgg ccgagccttt gtaggacgtg atcatcgtgt ccagggtatc gtagaggttc
     3901 gtgttggcct cgacgatggc caccgccggc cggcctgact cttcgagcgg ggtgtcgtcg
     3961 tgctggattt cctccaggcc aacccaccgc cctggctttt cctgctcatc cagcagcagt
     4021 acgtgatctc tgctcgtgct gcgcatcttc tcccgtacct catcgtggct gtcggttagc
     4081 ctggcgaccg gccagtcgga tgtctcgatg tccgagaccc ggctgaggct aagacgcttg
     4141 atagagggtc ctgagccgat gaagtcctgg acaaactcat cggcggggtc ggtaagaatc
     4201 ctctctggcg tatcatactg ggcgatcttg gactgatgct tgaggatcgc tatgcggtcg
     4261 cccatcttta tggcctcgtc tatgtcgtgg gtgacgaaga cgatggtctt gcggatctcc
     4321 tgctggagcc tcagaaactc gtcctggagc ctgtcgcggg tgatcgggtc tatcgccccg
     4381 aaaggttcgt ccatcaagag gaccggcgga tcggcggcca tcgcccgcgc cactccgata
     4441 cgctggcgct ggccgcctga gagctccttc gggtaacggt cgcggtagga gaggtctata
     4501 cccacggtct caagtagctc gtctatacgg tcggaggtgc gtttcttctc ccagccgagc
     4561 atccttggca ccgtcgcgat attgtcggcg atggtcatgt gcggaaagag cccgatctgc
     4621 tgtatgacgt atcctatcct acggcggagc ttgtccggat tgaccttcgt cacgtcctcg
     4681 ccctggagaa agattctgcc tgatgtcggt tcgatgatgc ggttgatcat cttcatggtc
     4741 gtggtcttgc cgcaccctga aggcccgacc aagactacga tctccccctc gtagatctcc
     4801 agcgaaagat catccaccgc ggcttcttcc tgggctggga agaccttggt gaggtcctcc
     4861 aggcggatca tggtttcagc ggtttgcgta ttattctctt ctgcggccct gtcagtcacg
     4921 tatacccctc gaagtcgtca gtttacccag caagttgaag aacgtgtcga acagtaacgc
     4981 cagcacgatg atgccgagaa tgccgccgat caccaggttg agggctgtgg ggctgccgat
     5041 gcgggcgagc ccggcgaaga tgtcgtcgcc caaacccgga ccgttcacca cggccgcgat
     5101 cgccgcgatg cccatgagta acatcgtgga gacgcgcacc ccggtgatga tgaccggcca
     5161 cgccaggggc agctcgatgc gcaggagccg ctccctggcg ttcatgccca ttccttgtgc
     5221 ggactctgcg atcgcagggt ccacggagcg cagaccggca acggtgttgc gcaagatcgg
     5281 cagcagcccg tacaggacca gcgcgacgac cgccggcagg tagccgaggc cgaagatcgg
     5341 gataaagagg ccgaacaacg cgaaagaggg gatggtaagg aaggtggagg tgatcgcgag
     5401 cgccacgttt gcggcgctct ctctacggta caccagcacg cccagcgcca ccccgatcac
     5461 gatgtcgata accagcgcta tggcgacgac tatagcgtga ccgatggcca gctctagcac
     5521 gtgatcccag ctaagggata tgtagtcgag aaagctgctc atcctgccca ccacctctcc
     5581 gcctggcgtc taaccacggc gatacactat aacttaacac cgacttccgt tgatcgggcg
     5641 atgctcgaag cgtacgaagc agcaccgcat atcaggaaac cccggaggca tcgtcggtag
     5701 cgggtcgagg atcagcggac cagccgagca gggtagagag atcgtccgct gccctcttga
     5761 cctcccttcc cgcggcttcc aaccgtccgc cgagacggaa cttgggagcc gagatgttga
     5821 gcgcggcgac gagcttcccc ttgaagtccc taacaggcgc cgccgcaccg accaggccga
     5881 attcgaactc ctcatcgacc agggcataac ctctctcgcg agcgacggcg atcctctcct
     5941 ccaactcttc gatgttgcga acggtgccag gcctcaacct acggaactcc acgcccgaga
     6001 gaaggctaga gagggcttcg ctgtcgtggt cgaacagaag cgcgcgcccc gacgaagtgc
     6061 aatagatgtg gaccatgcgg ccgacccagc catccgcttt gacggcgtgg gtcggggact
     6121 tggagagcac cgtcagaacc tccactcctt gcagaacgga gaggtgggcc gtttcgccga
     6181 cgttctctac gagccgctcg agtaacgtgg gcgtaacact tagcaacctc tgctcgctgg
     6241 cgcgcgccgc gagcgcgaag aatctccacc ccagcctgta ctggagggtc tcggtatca
//
LOCUS       DATMVF010000169        12754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_300853, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000169 DATMVF010000000
VERSION     DATMVF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300853"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1121
                     /locus_tag="VN178_08390"
     CDS             192..1121
                     /locus_tag="VN178_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HWS81029.1"
                     /translation="MTEQRGAVSGPDPAELWRQWMESGSKVWSEMAGAQGRQVDPYGA
                     YRRWFEGVQEIQERMVGTVPERVAGDPGVNDLWRRWFELSLDSWRKTAEMGQNAMEVL
                     PRWMQMLDEARTNLLAGGAPPSDPLQFAAQWYNATSGPFSDFVGDVIEREEFLEPSSR
                     FLQSYASFYKVFKRNSEDHLKNLQLPVRSDISRIAGLVVNLEEKVDRIEEVLEDFEYG
                     YAEPATTESIERLETRLDRVEGKLDRLLVALEGGIEDGGPRAETNGAGGEIKATDAAR
                     REAREMGVDLAEVAGTGADGQVTVEDVRKKGES"
     gene            1122..2222
                     /gene="phaC"
                     /locus_tag="VN178_08395"
     CDS             1122..2222
                     /gene="phaC"
                     /locus_tag="VN178_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HWS81030.1"
                     /translation="MTETANYGAVPTSGDAAIPADVENFFDKYSRGMKIVVEGAQADT
                     GQTPKETVWTKNKAKLYHYAPNAQKKHPVPILMVYALINRPYILDLMPGNSFIEYLVG
                     EGFDVYMLDWGVPGDEDKDLTFENYVLDYLPRAVKKVLRHSHSDELTILGYCMGGTMS
                     AMYASLFPEHLKNLILMTAPVEFPVGEMGLYSLFTSEKYMNPGLLADAFGNIPGDLID
                     TGNRMLKPVTNYVGTYVNMWERIFEEKPMETWLAMNKWVNDGPPFPGAAFRQWITEFY
                     QQNKLAKGEMILRGRRIDLSNIRVPLLNIAGKKDHICTLPQAEATMNLVGSEDKEFFV
                     LDAGHVGLMTGRGAKKGLWPKVSDWLSTRSGG"
     gene            complement(2358..4277)
                     /gene="phaC"
                     /locus_tag="VN178_08400"
     CDS             complement(2358..4277)
                     /gene="phaC"
                     /locus_tag="VN178_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HWS81031.1"
                     /translation="MSEGRTGNHGTEVPPEMAPFDAWSEWIRANMGPMTATPGASVPW
                     LMMPGVRTGEEAAELPEGAVRHDPLLSTVEKLWDANPIQNVLPVDWAEITRALQTLWA
                     REMSDPARAIERATDFNARLFGATMRSWQEATNRFWGLGTSQEEDEGRPDKRFSAPDW
                     EQNPFYETLKESYLLASEYLLDEAERTDGEDTPEQRRLRFHLKQFVDAMAPVNYLLTN
                     PAALKRAFETGGVSLAEGARNLLADLQEGRMSMTDANAFQPGENLAITPGKVVYRNEL
                     IELIQYEPTTEQVHETPVMFIPPWINKYYILDLQPKNSLVKYLVDEGFTVFMISWKNP
                     DASMEETKFDDYMTKGPLAAAEVVRDITGSEKVNPVGYCVGGTLLAIALSWLAAGDDE
                     NPFGPPTFMVALHDFTEVGDTGVFIDEPQIEFMEQQMLERGYLDNRKMANMFNLLRSN
                     DLIWSNVVNNYLLGQRPPAFDLLYWNSDGTRMARDAHSFYLRNTYLENNLIKPGKVEI
                     NGRPIDLSRIEGDIYAVGAEKDHIVPWASAWKISRLANGTTRFTLANSGHIAGMINPP
                     SKGKGRYWATENGGAFHTPAQWREQAKEHEGSWWEDWTKWLDAHSGEMVDPPEMGSET
                     YPPIEDAPGTYVKEK"
     gene            complement(4389..5285)
                     /locus_tag="VN178_08405"
     CDS             complement(4389..5285)
                     /locus_tag="VN178_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWS81032.1"
                     /translation="MPVTITESTSIDYDISGQGPPLLLINGLGFGRWGWFKQVPAFSR
                     HFRTITFDIRGERALENGVHDLVRDVVSLLDHLGVKKAHVLGASLGGFVGQELTLLRP
                     DLVDRLVLVCTSYGGRGPESMSVQALSDMLGWGSMSPEGVVRRGLETATSGTYRAEHE
                     DEFEQLVDWRLADSPSEASYYEQARAGAGFDLSRDVGRITSPTLVIHGAEDRYVPVAN
                     AAALAGAIPGARLRVLDHAGHLVFIERFADVNREVVTFLKPGRRGAQGRRTEPPTRLR
                     SLISGLPRSLGRGVRKLRDGIF"
     gene            5603..6826
                     /locus_tag="VN178_08410"
     CDS             5603..6826
                     /locus_tag="VN178_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS81033.1"
                     /translation="MTITPEGTLQGTRPVDLINAAGLLSDEEKRVRDLVREFVDREVI
                     PVAADYWDRAEFPFALLPGLGELGLMGGLLPEEYGCAGWNNVAYGLAIAELARGSGSL
                     ATFLHVQSGLAMTAIHELGSEEQKRRWLAPMAGCEKIGCFGLTEPEAGSDPGSLRTTA
                     VEKDGGYLLSGEKKWIGNASFSDVAVIWARTDDGKISGFLVETENPGFRADVLPRKAS
                     QRAAWQTHITLEDCHVPGDARLPKADGLGSTLSILTHSRYGVGWDGLGQATDCYEVAL
                     AYAKEREQFGQPIASFQLVQQKLVEMVNEISLSQLLSIHVGRLKDAGGLDPATVSMFK
                     MNNVAKARRIAALARDVLGGNGILLDYRVMEHMADIEGVYTYEGTNDVNTLIVGQAIT
                     GHRAFSPRPPRRAEE"
     gene            complement(7036..8250)
                     /locus_tag="VN178_08415"
     CDS             complement(7036..8250)
                     /locus_tag="VN178_08415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWS81034.1"
                     /translation="MSFGNGNGVGEIVISTPLRTGIGTFGGSLKDTPATELGATVGKE
                     VLSRAGIEGDQVDQIIVGNILSAGQGMNPGRQVGIKSGLPVEVPGMTLNRMCGSGLQA
                     IVSAAQEIALGDADVVMAGGIENMDRAPFLLDKGRYGYRMGMPKADIFDHMVYDGLWD
                     IFNDYHMGVTAENVAEEYGITREDSDAYAVRSHQRAAKAHEEELFDGQIVPVEVKQKK
                     ETVQFTTDEHIRPNASVEGLQKLKPVFKKEDGTVTAANASGINDGAAMMLVSSERKAE
                     ELGMPVAGRLVSAAVAGVDPSVMGTGMIPASRLALKKAGLSIDDIDVVEANEAFASIA
                     IAVERTLEVPEEKMNPVGGAVALGHPIGATGAVLTVKILHHLARTGGQYGLVTLCIGG
                     GMGIAAIFERVS"
     gene            complement(8357..8698)
                     /locus_tag="VN178_08420"
     CDS             complement(8357..8698)
                     /locus_tag="VN178_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81035.1"
                     /translation="MDQRQKQQVNEAAEKFADALKESYKSIADRTVTAQELNAQLTQD
                     FFNGVINNLRTQAEGNRALSEDLIEQQRKQREASQALAQESVNAYTDFLNSMFAYYKG
                     NLDEVQRRANQ"
     gene            complement(8839..9171)
                     /locus_tag="VN178_08425"
     CDS             complement(8839..9171)
                     /locus_tag="VN178_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81036.1"
                     /translation="MATTQKTNRAVERLAESTRDTYQTVVDHAVGLQERNVRFAQGMV
                     DGTIKELRHQAESNRSVVGELFERAEDQRDAYQTLVEQSIDAYMDLAYAPFTYYKEGL
                     EAARKATR"
     gene            complement(9202..9606)
                     /locus_tag="VN178_08430"
     CDS             complement(9202..9606)
                     /locus_tag="VN178_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS81037.1"
                     /translation="MNNEERETGGREREFRGVEARPRNWLVPVILLSLREWNSYGYEL
                     MERASAFGFEAMNPGTLYRTLRQMEKEGIVESSWETSRGGPARRMYTITDAGRTYLDF
                     WAKSLEQYQHTMDNFFRLYTGRPLKDKEESGE"
     gene            10137..10886
                     /locus_tag="VN178_08435"
     CDS             10137..10886
                     /locus_tag="VN178_08435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HWS81038.1"
                     /translation="MGTLKEAVVVVTGASRGLGRAIAEELAGGGAKVVVNYSRSKEPA
                     EELVDQISESGGEAIAVQADVSEAEQAQKLIDQALERFNRVDVLINNAGINIDRTLRK
                     LSVEDWDKVIQVDLNSAFYTVHAVLEHMTDQGGGKIINMSSFVGEAGNIGQANYSAAK
                     AGLLGFTKTAAQELARFGITVNAICPGFIETDMVANIPEEAQQKLLKQVPLGRFGKPE
                     EVARTVRYLIEDGDYITGQSIDINGGIYIRT"
     gene            11135..11902
                     /gene="fabG"
                     /locus_tag="VN178_08440"
     CDS             11135..11902
                     /gene="fabG"
                     /locus_tag="VN178_08440"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWS81039.1"
                     /translation="MRLDGRTAVVTGSARGIGAAEAIRLAQEGANVAVLDLSAEACRE
                     TVEAIEAAGSEAIAIACDVSSAQQVEKAFEEIAHRFGRIDILVNNAGLLRDNLSFKMS
                     EDDWDKVLDVHLKGSFLCSKAAQKYMVDQEYGKIVMTSSIVALGNKGQANYSAAKAGL
                     QALARTLALELGRFNINVNAVAPGWIETEMTKEAAERVGMTMEEMKDRFAKNIPLKRF
                     GRVDDIANVVAFLVSDDASYISGETIYVAGGPSSSVI"
     gene            complement(12160..>12754)
                     /locus_tag="VN178_08445"
     CDS             complement(12160..>12754)
                     /locus_tag="VN178_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWS81040.1"
                     /translation="YHCIKTSGALKDGESVLVHAAAGGVGTLSVQMAKLLGAGTVIAT
                     ASSEEKLDLARSLGADVLIDYTEEDWPEKVREATEGRGADVILEMVGSDFPEKNLTCL
                     NVFGRMVVFGAASRERGTIVPATLMKRCHAIVGFYLPQIMRRPDLFVPSLREVLGWIS
                     SDKLKLTIGSTYPLAEAAEAHSALEGRQTTGKLLLNP"
BASE COUNT         2382 a         3951 c         3948 g         2473 t
ORIGIN      
        1 gaacgggaga agctgaaagc tgagagcgaa gcgtgctgac aagacgccaa agacgccgtg
       61 ctgaagtgct ggtatgtgcc gttaacacat acgtttcgat tgattgtata attggcatgc
      121 tcggcgctcg ctgcggtgag agcgaggagg gtgccagcga cggatattgt ttggtgacga
      181 ggaggtttcg gttgacggag cagagaggag cggtgtccgg ccctgaccct gccgagctct
      241 ggcgccagtg gatggagtcg gggagcaagg tgtggtcgga gatggccggt gctcaggggc
      301 gccaggtcga cccttacggg gcttatcgcc ggtggttcga aggcgtgcag gagattcagg
      361 agcggatggt cgggacggtc cccgagcgtg tcgccggcga tcctggggtc aacgacctgt
      421 ggcggcggtg gttcgagctg agcctggact cctggagaaa gaccgccgag atgggtcaga
      481 acgcgatgga ggtactgccg cgctggatgc agatgctaga cgaagcgcgt accaacttgc
      541 tggccggagg cgcaccgccg agcgacccct tgcagttcgc agcccagtgg tacaacgcca
      601 cgagcggtcc cttctcggac tttgtgggcg acgtcatcga gcgtgaggag ttcctcgagc
      661 cttcgagccg ctttctacag agctacgcca gcttctacaa agttttcaag cgcaactccg
      721 aggaccacct caagaacctc cagctccccg tccgctcgga catctccagg atcgccggcc
      781 tcgtcgtaaa cctggaggag aaggtggatc gcatcgagga ggttctcgag gacttcgagt
      841 acggctacgc cgagccagcc acgaccgagt cgatcgagag gctcgagacg cgactcgaca
      901 gggtcgaggg caaactcgat aggctgctcg tagcgctcga aggcggtatt gaggacggcg
      961 gcccacgggc ggagaccaac ggtgcgggcg gcgagatcaa ggccactgat gccgcgaggc
     1021 gcgaggcgcg tgagatgggc gtcgatttgg ccgaggtagc agggaccggg gccgacggtc
     1081 aggtcaccgt cgaggacgtg cgcaagaagg gagagagcta gatgaccgag acggccaact
     1141 acggtgcggt tcccacctcg ggcgacgccg ctataccggc ggacgtagag aacttcttcg
     1201 acaagtacag ccgcgggatg aagatcgtcg tcgagggcgc ccaggccgac acgggccaga
     1261 cacccaagga gacggtctgg accaagaaca aggcgaaact ctaccactac gcccccaacg
     1321 cccagaagaa gcatcccgtc ccaatcctca tggtctacgc cctcatcaac aggccttaca
     1381 tactcgacct catgccgggc aacagcttta tcgagtacct tgtaggcgag gggttcgacg
     1441 tctacatgct ggactggggc gtgccgggcg acgaagacaa ggacctcacc ttcgagaact
     1501 acgtactcga ctatctgccg agggccgtaa agaaggtcct caggcactcg cactccgacg
     1561 agctcaccat actcggctac tgcatgggcg gcacgatgag cgccatgtac gcctcgctgt
     1621 tccccgagca cctgaagaac ctcatcctca tgaccgcccc cgtggagttc cccgtagggg
     1681 agatgggtct ctacagcctg tttacgagcg agaagtacat gaaccccggg ctgctcgccg
     1741 acgccttcgg caacatcccc ggcgacctca tcgacacggg caacaggatg ctaaagcccg
     1801 tgaccaacta tgtcggcacc tacgtgaaca tgtgggagcg catcttcgag gagaagccga
     1861 tggagacctg gctcgcgatg aacaagtggg tcaacgatgg gccccccttc ccgggcgcgg
     1921 cgttcaggca gtggatcacg gagttctacc agcagaacaa gctggccaag ggcgagatga
     1981 tcctccgggg ccgccgcatc gacctctcga atatcagggt ccccctacta aacatcgccg
     2041 gcaagaagga ccacatctgc accctcccgc aggccgaggc cacgatgaac ctcgtcggca
     2101 gcgaggacaa ggagttcttc gtcctcgacg caggccacgt cggcctcatg acgggccgcg
     2161 gcgcaaagaa aggactctgg cccaaggtca gcgactggct gagcacccgc tctggcgggt
     2221 agcggaaggg caagccctcc cgctacccgc gctggtcagc atttcagcac gccgcccggc
     2281 ttcgccgggc ggcttttcag cattccagcg tgcctgccgt cgggtcttcc ttatgcctga
     2341 aacctgtagc ctgataccta tttctccttc acgtaggttc ccggggcgtc ctctataggc
     2401 gggtacgttt cgctgcccat ctcggggggg tctaccatct ctccggagtg ggcatcgagc
     2461 cacttggtcc agtcttccca ccaggagccc tcgtgttctt tggcctgttc gcgccactgt
     2521 gcgggggtat ggaaggcgcc gccgttctcg gtcgcccagt agcggccctt gcccttggat
     2581 ggggggttga tcatccccgc tatgtggccc gagttggcga gggtgaagcg ggtggtgccg
     2641 ttcgcaagcc tggagatctt ccacgccgag gcccagggga ctatgtggtc cttctcggcc
     2701 cccacggcgt agatgtcgcc ctcgatcctg gagagatcga tgggacggcc gtttatctct
     2761 accttgcctg gctttatgag gttgttctcc aggtaggtgt tgcgcaggta gaagctgtgg
     2821 gcgtcgcggg ccatgcgcgt gccgtccgag ttccagtaaa ggaggtcgaa ggcggggggc
     2881 ctctggccga gtaggtaatt gttgacgacg ttggaccaga tcaggtcgtt cgagcgtagc
     2941 aaattgaaca tgttggccat cttgcggttg tcgagataac cgcgctcgag catctgctgc
     3001 tccatgaact ctatctgcgg ctcgtctatg aagacgcccg tgtcccctac ctccgtgaag
     3061 tcgtgcagag cgaccataaa ggtcggcggg ccgaaggggt tctcatcgtc gcccgcggcg
     3121 agccaggaga gcgcgatcgc gagcagcgtc cctcccacgc agtagccgac ggggttgact
     3181 ttctctgatc ccgtgatgtc cctcacgacc tcggccgcgg cgagcggtcc tttggtcatg
     3241 taatcgtcga acttggtctc ctccatcgag gcgtccgggt tcttccagga gatcatgaac
     3301 accgtgaagc cctcgtccac caggtacttg accaggctgt tcttcggctg cagatcgagg
     3361 atgtagtact tgttgatcca gggcggtatg aacatcacag gcgtctcgtg cacctgctcg
     3421 gtcgtcggct cgtactggat caactctatc agctcgttgc gatagacgac cttgcccggt
     3481 gtgatggcca ggttctctcc cggctggaag gcgttcgcgt cggtcatgct catgcgacct
     3541 tcttgcaggt ccgcgagcag gttgcgggcg ccctcggcga ggctgactcc cccggtctcg
     3601 aaggcccgct tgagggctgc ggggttggtc agcagatagt tgacgggagc catggcgtct
     3661 acgaactgct tgaggtggaa cctcagacgt cgctgctcgg gcgtatcctc tccgtcggtc
     3721 ctctcggcct cgtcgagcag atactccgag gcgagcaggt atgactcctt gagtgtctcg
     3781 tagaacgggt tctgctccca atcgggcgcc gagaaccgct tgtcgggacg cccctcgtcc
     3841 tcctcctgcg atgttccgag accccagaaa cggtttgtgg cctcctgcca gctcctcatc
     3901 gtggcgccga acagcctggc attgaagtcc gtggccctct ctatggcgcg cgcggggtcg
     3961 gacatctcgc gcgcccagag tgtctgcaac gcgcgggtga tctcggccca atccacgggc
     4021 agaacgttct gtatggggtt ggcgtcccat agcttttcga cggtggagag caaggggtcg
     4081 tgcctaacgg ccccctcggg taactccgca gcctcttctc ccgtccttac gcccggcatc
     4141 atgagccagg gtacgctcgc gcccggcgtc gcagtcatag gacccatgtt cgcccggatc
     4201 cactccgacc atgcgtcgaa cggcgccatc tcgggcggaa cttcggttcc gtggttaccg
     4261 gtcctgcctt cgctcatcta gtcttcctcc tcagagattc acccggcgga tctccctcca
     4321 ccggttcgtg ttccgattta ctatggtacc cattctgcca gcatcttata ggcggccggc
     4381 gggcggcatc agaagatgcc gtcccgcaat tttcggaccc cacggccgag agaccgtggc
     4441 aaccccgaga tcaaggaccg taacctggta ggcggctccg tccgccgtcc ttgggcgcct
     4501 cgccttccgg gcttcaggaa ggtgaccacc tccctgttta catcggcgaa ccgctcgata
     4561 aagacgaggt ggcccgcgtg gtcgaggacg cgcaacctgg cgccggggat ggccccggcg
     4621 agggccgccg cgttggcgac cggcacgtaa cggtcctcgg cgccgtggat cacgagcgtg
     4681 ggcgaggtta tgcgccccac atcccgggag aggtcgaagc cagcccctgc cctggcctgc
     4741 tcgtagtaag aggcttcgga gggcgaatca gcgagccgcc agtctacgag ctgctcgaac
     4801 tcatcttcgt gctcggcgcg atacgtcccc gaggtcgccg tctccaagcc tcgcctgacg
     4861 actccctccg gagacatgga cccccatcca agcatgtcag acagggcctg cacagacatc
     4921 gactcgggcc ccctgccacc gtagctggtg cagaccagaa ccaaccgatc gacgaggtcg
     4981 ggcctgagca gagtcaactc ctgccccaca aagccgccga gggaagctcc gagcacatgg
     5041 gccttcttta cacccaggtg gtccagcagc gacaccacgt cacggaccag atcatggacc
     5101 ccgttctcga gcgcgcgctc gcccctgata tcgaaggtga tcgtccggaa gtggcgcgag
     5161 aaggcgggaa cctgcttgaa ccaaccccag cgcccaaagc caaggccgtt tatgaggagc
     5221 aggggcggcc cttgcccgct gatgtcatag tcgatgctcg tgctctcggt gatcgtcacc
     5281 ggcattcgct actcacctcg attctcgcac cacaaagagc caggcggcca gaaacagaac
     5341 cggttatccg gatccagcgc cttcctctgt agaatagatg cactctgcac ttagatgcaa
     5401 catgagaata ttctagtggg tctgatatga ggagcaagtt ggtagcgtga aaggcatcac
     5461 tctggggagg cttcaggtgt tgggggcgcg ggccggagcc cgtccacttc gagaacgttc
     5521 cagattgacc gagtcgactc tggaacctat gctcttgttg gattggcgcc tggagtctga
     5581 gtgtggagga gagcctgaga acatgaccat tacgccggag ggaacgctgc aagggacgag
     5641 accggtcgac ctcataaacg ccgccggcct tctttcggat gaggagaagc gggtcaggga
     5701 cctggtccgc gagtttgtcg accgggaggt catcccggtt gcggcggact actgggacag
     5761 ggcggagttc cctttcgcgc tcctccctgg gcttggggag ctcggcctca tgggtggcct
     5821 cctccccgag gagtatggct gcgccggctg gaacaacgtg gcttacgggc tcgcgatagc
     5881 cgagctggcg cgggggtctg ggagcctggc gacgtttttg cacgtccaga gcgggctggc
     5941 gatgacggcc atacacgagc tgggatcgga ggagcagaag cggcgctggc tggcccccat
     6001 ggccgggtgc gagaagatcg gctgtttcgg cctgacggag ccggaggccg ggagcgaccc
     6061 tggttcgctg agaacgacgg ccgtagagaa ggacggcggc tacctgctaa gcggtgagaa
     6121 gaaatggatc ggcaacgcct ccttctcgga cgtggcggtg atctgggcca ggaccgatga
     6181 cggcaagatc tccggtttcc tcgtggagac cgagaatccg ggcttccggg ccgacgtcct
     6241 cccccgtaag gcctcccagc gcgccgcctg gcagacgcac ataacgctgg aagactgcca
     6301 cgtgcccggg gatgccaggc tgcccaaagc agatggcctt ggctccacac tctctatcct
     6361 cacacattct cgctacggcg ttgggtggga cgggctcgga caggcgacgg actgctacga
     6421 ggttgcgttg gcgtatgcga aagagcggga gcagttcggg caacctatcg cttccttcca
     6481 gctcgtccag cagaaactcg tggagatggt caatgagatc tcactcagcc aactcctctc
     6541 catccacgtc ggtcgcctca aggacgcggg agggctagat cccgccacgg tctcgatgtt
     6601 caagatgaac aacgtcgcca aggcgcgccg gatcgccgcg ctggcgcgag acgtactggg
     6661 gggcaacggc atcctgttgg actaccgcgt gatggagcac atggcagata tcgagggcgt
     6721 ctacacctac gagggcacca acgacgtcaa caccctcatc gtgggccagg ccataacagg
     6781 ccaccgcgcc ttctcccccc gccccccgcg tcgggccgaa gagtagctag tcagcacgct
     6841 ccagccttcg gctgtcgctt gtcagcactt cagctagtca gctttttgct ttctatcgcg
     6901 ttctcagcgc atttacctgc cggcgtcgtt cccgagcggg ccgtctcgac aagccaaagg
     6961 gccgaggctc aacgcctcgg ccctagctga ccaactgaca agtcgcctgg aacaggcgac
     7021 gtgctgaaat gctgactagc tgactcgctc gaagatcgcg gctatgccca tgccgccacc
     7081 gatgcagagc gtcaccaggc cgtactggcc gcccgtgcgg gcgaggtggt gaagtatctt
     7141 gacggtcagg acggcgcccg tggccccgat ggggtgtccg agggccaccg ccccgcctac
     7201 cgggttcatc ttctcctcgg gcacctcgag cgtacgctcc acggcgatag cgatcgacgc
     7261 gaaggcctcg ttcgcctcga cgacgtcgat gtcgtcgatg gagagcccgg ccttcttgag
     7321 ggccagcctg gaggccggga tcattccggt tcccataacg gaagggtcga caccggccac
     7381 ggccgcggag accagcctgc ccgccacggg catcccgagt tcctctgcct tacgctcgct
     7441 gctcacgagc atcatggcag caccgtcgtt gatgcccgag gcgttcgcgg cggttaccgt
     7501 gccgtcttcc ttcttgaaga ccggcttcag cttctggagg ccctccacgc tcgcgttcgg
     7561 gcgtatgtgc tcgtccgtgg tgaactggac ggtctccttc ttctgcttga cctcgacggg
     7621 tacgatctgg ccgtcgaaga gctcctcttc gtgagccttg gcggcccgct gatggctgcg
     7681 cacggcgtag gcgtcggagt cttcgcgggt tatgccgtac tcctcggcga cgttctcggc
     7741 cgtcacgccc atgtggtagt cgttgaagat gtcccacagg ccgtcgtaga ccatgtggtc
     7801 gaagatgtcc gccttgggca tccccatcct gtagccgtaa cggcccttat ccagcaagaa
     7861 aggcgccctg tccatgttct cgataccgcc ggccatgacc acgtcggcgt cgcccagggc
     7921 tatctcctgc gccgcagaga ctatggcctg aagccccgag ccgcacatcc tgttcagggt
     7981 catgcctggg acctccacgg ggaggccgct ctttatgccg acctggcgtc cagggttcat
     8041 gccctggccc gcggagagga tgttaccgac gataatctgg tcgacctggt cgccctcgat
     8101 ccccgcgcgc gagaggacct ctttgccgac cgtggccccg agttccgtcg ccggcgtgtc
     8161 cttgagcgaa ccgccgaaag tccctatacc cgtcctgagt ggcgtcgaga tgactatctc
     8221 gccgacaccg tttccgttgc caaagctcac cttatctcct tccgtctctt ccaggtaaga
     8281 ggatacatca aaagacaagg cccagacttt gaggcccggg cctttcaata cgctcttcca
     8341 gtgtctcgcc caccgcctac tggttggccc tgcgctgcac ctcgtccagg ttgcccttgt
     8401 agtaggcgaa catggagttc aggaagtccg tgtaggcgtt caccgactcc tgggccagcg
     8461 cctgggaggc ctccctctgc ttgcgctgtt gctcgatcag gtcctcagac agggccctgt
     8521 tgccctcggc ctgggtcctc aggttgttga taacaccgtt gaagaagtcc tgcgtcaact
     8581 gggcgttgag ctcctgggcc gtgacggtgc ggtccgcgat ggacttgtag gattctttca
     8641 gcgcatcggc gaacttctcc gccgcttcgt tcacctgttg cttttgtctc tggtccacga
     8701 tctcacttcc tctatctaat cctacatatg tactagaacc tacccgatat cagggcagcg
     8761 aaacatcaaa gagaccgaaa agtgaggagc ggacccgaaa gcccgccccc aaacgttcga
     8821 ccctgataga gctacgcttt acctcgttgc cttcctcgcg gcctccaggc cctccttgta
     8881 gtaggtgaag ggcgcgtaag cgaggtccat gtaggcgtcg atggactgct cgacaagggt
     8941 ctggtaggcg tcacgctggt cctcggcgcg ctcgaagagc tcgcccacga cgctgcggtt
     9001 gctctcggcc tggtggcgca gctccttgat ggtgccgtcc accatgccct gcgcgaacct
     9061 gacgttccgc tcctgaaggc ccacagcatg gtccactacc gtctggtagg tgtcccgcgt
     9121 gctctcagcg agcctctcta cagccctgtt ggtcttctgc gtagtggcca tctctatctc
     9181 ctttctcgcg tatgacgcga actattcgcc actctcttcc ttgtccttca acggcctgcc
     9241 agtgtacagc cggaagaagt tgtccatggt gtgctggtac tgctccagcg acttggccca
     9301 gaagtcgagg tacgtcctgc ccgcgtccgt gatggtgtac atcctgcgcg caggcccacc
     9361 cctcgaagtc tcccaactgg actcgacgat gccctctttc tccatctgcc ggagcgtcct
     9421 gtacaacgtt ccagggttca tcgcctcgaa cccgaaagcc gaagcccgct ccatgagctc
     9481 gtacccatac gagttccact cccgtagtga gagcaggatc accggcacca gccagtttct
     9541 cggccgcgcc tcgaccccac ggaactctcg ctccctgcct cctgtctccc gctcttcgtt
     9601 gttcattgcg ctctcttcca tatacctatg tgcattttac atatatgtat caatctgtca
     9661 aggtttagct gaggctctca ttccgagctt tcctttcaac aacgagaact tcgctccgaa
     9721 gccggacctc tggtttacga accctttatg agggcgttcc gcgcatatct gtgtgtcgaa
     9781 cataagtacg cagtacatct tagcccaaat ccgatgggct gtttatcgaa attacataga
     9841 aaggtgcatg acccgccctg gatagcgatg taagctgggc gtactccgta cgcgcggagg
     9901 agggaaccgg cgtccgtgac tactcctttc ccattctcct actatcaccc caccagcacg
     9961 gacgccgatt ctaccgcctc cggcatcgtc ccacgcttcg tctgcacggt gccgggatct
    10021 ttctggacct ggagcaggag tcgacatgca ccgtctcggt gctttgaccg atcttgcacc
    10081 cgaatgccgt agtcaataaa ctccgtctca catgcgaaca gcaaaaggag gatagcgtgg
    10141 gcaccttgaa agaagccgtt gtcgtcgtga ccggcgcgag caggggtctg ggcagagcca
    10201 tagccgaaga gttggctggt ggaggagcca aagtcgtcgt caactactcc aggagcaagg
    10261 agcccgccga ggagttggtc gaccagatct ccgagagcgg tggagaagct atcgcggttc
    10321 aggccgacgt ctcggaagcg gagcaggccc agaagctcat agatcaggca ctggagaggt
    10381 tcaaccgggt agacgtcctg atcaacaacg ccgggatcaa catagaccgc acgctcagga
    10441 agctctccgt ggaggactgg gacaaggtca tacaggtaga cctcaacagc gccttctaca
    10501 cggtccacgc ggtgttggag cacatgacgg accagggtgg gggcaagatc atcaacatga
    10561 gctccttcgt cggagaggcc ggcaatatcg gacaggctaa ctactcggcg gccaaggccg
    10621 ggctgctcgg cttcaccaag actgcagccc aggaactggc ccgcttcggg atcaccgtca
    10681 acgccatctg tcccggattc atagagaccg atatggtcgc caacatcccc gaggaagccc
    10741 agcagaaact cctcaaacaa gtgcccctgg gccgcttcgg gaaaccggaa gaggtcgcca
    10801 ggaccgtccg ctacctgata gaagacggtg actacatcac aggccaatcc atagatataa
    10861 acggcggtat ctacatcagg acgtagcgag agagcgcccc gcctacccac gggcggggct
    10921 tcctcccaaa gcaggctgcg caatcctcca gataggcagc ccaccccgta acgcgtacgc
    10981 ctccccggag tgagccagca gagaatgcat ccaaaggtgt acatatctgt gctggacaca
    11041 tacgttcaat tcgtatacaa ttaactgtca tgtaacaaag acgacaccct ccttgggtgc
    11101 agacggtagt gttacctaac cgagaggtgg acctttgagg cttgacggac gaacggcggt
    11161 tgtaacgggg tctgcgcgcg ggatcggggc ggctgaggcc atccggctgg cgcaggaggg
    11221 ggcgaacgtg gcggtcctgg atctctcggc ggaggcttgc cgggagacgg tggaggctat
    11281 cgaggctgcg gggtcggagg cgatcgcgat cgcctgtgac gtatcgagtg cgcagcaggt
    11341 ggagaaggcg ttcgaggaga tcgcacaccg ttttgggcgg atcgacatcc tcgtcaacaa
    11401 cgccgggctg ttgcgcgaca acctctcctt caagatgagc gaggacgact gggacaaggt
    11461 gctcgacgtg cacctcaagg ggagcttcct ctgttcgaag gccgcgcaga agtacatggt
    11521 agatcaggaa tacggcaaga tcgtgatgac cagctccatc gtggccctgg ggaacaaggg
    11581 ccaggccaac tattcggcgg ccaaggccgg cctccaggcg ctcgcgcgga cgctggcgct
    11641 cgagctcggg cgtttcaaca tcaacgtcaa cgctgtggcc ccgggttgga tcgagaccga
    11701 gatgaccaag gaagccgcag agcgggtcgg gatgaccatg gaggagatga aggatcgctt
    11761 cgccaagaac atccccctca agcgcttcgg tagggtggac gacatagcca acgtcgtagc
    11821 attcctcgtc tccgacgacg cctcgtacat cagtggggag acgatttacg tcgccggtgg
    11881 accttcgagt tcggtgattt agagcaggca gccttcagca gtaagcaatc agctttttgg
    11941 gcggggtact tctccccgcc ctttcgctta cttgcaagat ggtccttacg gaatttgtcg
    12001 ctgggggcgc tgagcgcgtc caaacagagt cggcctacca ggttacgcgt gttatggggg
    12061 attgcgtgac ttcgagccat aacccgaagt cggcggacta ctcggaaatc cggaagctga
    12121 aagctgatcg ctgatggcgc ggttcgcatt gcgaatcgcc tacgggttga gtagcaactt
    12181 acctgtcgtc tggcggcctt ccagggcgga gtgggcttcg gcggcttcgg cgagtgggta
    12241 ggtgctgccg atggtgagtt tcagcttgtc cgaggagatc caaccgagca cttcccggag
    12301 gctcgggacg aagaggtcgg gacggcgcat gatctgcggc aggtagaaac cgacgatggc
    12361 gtggcaccgc ttcatcaggg tggcgggtac gatggtcccc ctctccctgc tcgccgcgcc
    12421 gaagacgacc atcctgccga agacgttcag gcacgtgagg ttcttctccg ggaaatcgct
    12481 gcccaccatc tcgaggatca cgtccgcgcc tctgccttcg gtggcttcgc ggaccttctc
    12541 gggccagtct tcctccgtgt agtctatgag gacgtcggcg ccgagggagc gggccaagtc
    12601 caacttctcc tcgctgctcg cggtggcgat tacagtcccg gccccgagga gcttcgccat
    12661 ctggacggag agggtgccca cgccgccggc cgcggcgtgt acgaggacgc tctcgccgtc
    12721 cttcagggcg cccgaggtct tgatgcagtg atag
//
LOCUS       DATMVF010000170        14011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_64997, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000170 DATMVF010000000
VERSION     DATMVF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14011
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64997"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VN178_08450"
     CDS             complement(<1..639)
                     /locus_tag="VN178_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81041.1"
                     /translation="MQLENKVAAVTGSSLGIGRAIALAFAREGASVAINYRSHPEEGQ
                     EAVEEIEKNGGKAISVQADVSEPDGVKRLIQQTVNEFGRLDVMVNNAGIEQKIPFLET
                     PLEVWEKVISVNLMGVWLGCQEAARQMVSQGDGGRIINVSSVHEDLPMPTNSPYCAAK
                     GGVRMLMRTIAVELAPHGITVNNIAPGAIETPINKNLDEHPEQRQELLKEIPL"
     gene            complement(623..2407)
                     /locus_tag="VN178_08455"
     CDS             complement(623..2407)
                     /locus_tag="VN178_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWS81042.1"
                     /translation="MPGRIEDYAMVGDLQTAALVGSDGSVDWLCFPRFDSPACFAALL
                     GNDSHGRWRLAPTSGGRCTRRRYRGHTLVLETEWETPTGAMRVIDCMPPRGVAPDLVR
                     VVEGVRGRVSVTSRLRIRFDYGHVVPWVTTEGREVRAVAGPDALWLRASAPHEEVGGE
                     ILSRFTLSEGERVPFVLTWAPSFGERPVAIDGLQAVDDTERFWLDWAGGISYEGRNQD
                     AVYRSLLTLKGLTYRPSGGIVAAATTSLPETLGGSRNWDYRYCWLRDSSFTLGALIGA
                     GLLEEARAWQQWVLRAVAGDPADAQIMYGIDGARRLPEYELPWLPGHEGATPVRVGND
                     AAGQVQGDVPGEVIGAAHVGRTAGIVPVERGWELQRWLAGRLEEAWTRPDNGIWESRG
                     EMQHFVYSKVMCWVAFDSLVKGVERFGLDGPVERWRAVRDEIHADVLEKGYDASRGTF
                     TQAYGSTALDACALLIPRMGFLPYDDERVISTVETIQSELTEDGLVLRYRTDQSDDGL
                     TGREGSFLICSFWMVYALWGIGRKREAEELFDRLLALRNDVGLLSEEYDTRAQRMVGN
                     FPQAFTHLALVNCALRLSEREVSSATRE"
     gene            2607..3635
                     /locus_tag="VN178_08460"
     CDS             2607..3635
                     /locus_tag="VN178_08460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWS81043.1"
                     /translation="MVGNGTAPTFGLWYDFRQRLPLGDYARFYAECLEEIEEGERLGF
                     AGIWLSEHHFVDDGYLPSPLVAAAAIAARTRTMTIGTNVLLLPMHHPLRVAEDAAVVD
                     LISGGRFVLGVGQGYVQHEFETLGFERKYRPSLFEEGIEVIRHALGEGRTGYEGRRWR
                     FDDLPFGPRPDGRLPLYIGAFADPAIDRAARLADGFLASAGGGAFGETHRKVLAALER
                     HGRAGEAFPYVASGVLFVDEDAGRAREIVGPAIAYQRTRYAEWGTDRDKPRPGPIREE
                     DLPWERYLVGTPEEVAQALIELYQEAPYDHLCFWGRLPGVTHEQALANMRLFASTVVP
                     RVRDAVEV"
     gene            3632..4696
                     /locus_tag="VN178_08465"
     CDS             3632..4696
                     /locus_tag="VN178_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HWS81044.1"
                     /translation="MRAIPGVLDEDRTAFLVRQRVARLATSDGMGKPHAVPVCFAMAR
                     ASIFIALDEKPKDVPVTRLKRVRNILENPEVALIADRYAEDWELLAFVMVRGRAELLD
                     PGNEEHTAAVRLLRGKYHQYEAMRIEENPIIAIRPERAASWGALDAPESSEPQILDTI
                     HGRRSVRKYLEKEVSEDLVERVLEAARWAPSPHGRQPWRFAVFTKEATKERLARAMGE
                     EWRANLEMDGQSVEIVEKRLEGSRRRLLEAPVLVLICLYLEDLDAYPDAERQCGERTM
                     AVQSLGAAAQNALLAAYDSGLDAGWMCAPLFCPERVVEALGLDPSLVPHALLTLGYAE
                     GDPPKRRGRKSLDELVVYRD"
     gene            4894..5775
                     /locus_tag="VN178_08470"
     CDS             4894..5775
                     /locus_tag="VN178_08470"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWS81045.1"
                     /translation="MTEQEIQEKGYANPDALVSTQWVAENLDDTENVRIVESDEDVLL
                     YDIGHVPNAVKIDWVEDLNDPLMRDYLDPEHFASLMAEKGISPDTTVVFYGDRNNWWA
                     TYALWVFRLFGHENVKVMDGGRVKWEAEGREMSQETPSFPNAQYPTPQRDDSKIRAFR
                     EDVMAHLEKVRSSEGGLIDVRSPGEYRGELLHMPDYPQEGALRGGHIPGAANVPWARA
                     ANEDGTFKNAQELREIYEGEAGLSPDEDTIAYCRIGERSSHTWFVLSYLLGYDNVKNY
                     DGSWTEWGNTVRAPIER"
     gene            5780..6232
                     /locus_tag="VN178_08475"
     CDS             5780..6232
                     /locus_tag="VN178_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWS81046.1"
                     /translation="MAAKPGASDRKRNGAALLVEHAKNPRHKNVEGVQEEADVVVPGG
                     SPECGGSVVVYLKGGDDGKIERLSWTGQGDTISMGATSIVLERILDEGLSMEEILEMD
                     YEEFVDSLGREVIGSRRRHATLGLSTIKGAIRIYQRKSRSAVKERAGG"
     gene            6471..7916
                     /gene="argH"
                     /locus_tag="VN178_08480"
     CDS             6471..7916
                     /gene="argH"
                     /locus_tag="VN178_08480"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWS81047.1"
                     /translation="MSDAGENTSGRLWGGRFGASPAEAFERLNASIPFDIRLAPHDVR
                     GSIAHARMLGVQGIISDGESAMLVSGLEAVLREVEAGEFTWDLSDEDVHTAVERRLKE
                     LVGDVALKLHTGRSRNDQVALDLHLFARDAAERISSDILAAMRALLGVAETNKDLILP
                     GYTHLQRAQPILLSHHLLAQFEALRRDLRRFEAAREAANVSPLGAAALGGTPHPVDPR
                     LTAEELGMEPFTNSLDAVSERDFALDLLYACAVLGVHLSRLGEEWVLWTSAEFGFATL
                     DDAYSSGSSIMPQKKNPDAYELMRGKTGRLIGDLNAVLITLKGLPLGYSKDLQEDKEP
                     LFDAVDSMLAMLAILPEMLRTARFHGIRMAEAAGGFALATELADFLAAGDVPFREAHH
                     AVGRLVRRCEELGVSLEDVPQVELAAAHPALSEVPEGLLTPRGSVRNKRSAGSTAPAS
                     VDEQLRQAKAFLETVESHSKGHLSEGSDRCG"
     gene            7907..8728
                     /locus_tag="VN178_08485"
     CDS             7907..8728
                     /locus_tag="VN178_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWS81048.1"
                     /translation="MRLVQMSDVHVGSGLFRPDLLEATIEESNALEPDLVAIVGDLTM
                     EGYRWEFEEAREFFDRLECPNVLYAMGNHDAKNVGYRHFEEFFGIRERSARIPVPEGE
                     AKVVVLDSTKPDLDEGEVGREHYSWLDSEFRGWDRGPRILMIHHHILAVPGTGRDVNN
                     LRDAGDVMAILRELKVDMVLSGHRHVPYVWSISGVRIVHSGTVSSMRVRGTMPPSYNV
                     IEIDEQNVSITLHQPGMGEAGREPLASFSRTPVTTSEFYPDFERFVLYDRLPFLK"
     gene            8925..9239
                     /locus_tag="VN178_08490"
     CDS             8925..9239
                     /locus_tag="VN178_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWS81049.1"
                     /translation="MVWRLKSFVAEFRSAFPDFHDSIDIQVAEGELVVTRFTSMGTHR
                     GTFMGVEPTNRELVWTGITIDRVSGGKIVERWANWDMMGMMQQLGAISSSGEISEEAS
                     PT"
     gene            complement(9282..9638)
                     /locus_tag="VN178_08495"
     CDS             complement(9282..9638)
                     /locus_tag="VN178_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81050.1"
                     /translation="MPTTVRIKPELITAHRLRIEMFGLEDEDIENTIRMKGWAWVLAR
                     RGWVYAGEPDFIYRQIREVVIALPDITFEPEAIEESVKTVLEKARTEEEREEGRLLLR
                     QAFEKTGQLSEAEEFL"
     gene            complement(9673..10908)
                     /gene="hutI"
                     /locus_tag="VN178_08500"
     CDS             complement(9673..10908)
                     /gene="hutI"
                     /locus_tag="VN178_08500"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HWS81051.1"
                     /translation="MAEATLLIHDLAAAVTPAGSGPLRGRGFGALRIHSPASIAIAGE
                     RIAAVGPPAEICHDLPSGVDYESVDGRGKVALPGLVDCHTHPAFLGDRAAEFELRSSG
                     ATYEEIHASGGGIRSTVAATRAGSERDLRSSVERHLGWMLEHGTTTAEAKSGYGLDRE
                     TELKSLRAIQDAGRRSPVDVYPTFLGAHTIPPEFDSAAEYVEFVINEVLPEAAPLAGS
                     ADIFLERGSFEVPEARRYLEACQEYGLALRLHADQFSERGAIPLALELGARSVDHLEA
                     TKHEAASLLARSDVAAVLLPACGLFLDLPLPPARLLADEGAIVALATDFNPGSSFCES
                     LPLVMNLACTRLGFSPAEALCACTANAAHVLGAADVGRLAPGYKADVLLLDAPAWHHI
                     AYHLGGDRFAAKIKSGRVL"
     gene            complement(10909..11739)
                     /locus_tag="VN178_08505"
     CDS             complement(10909..11739)
                     /locus_tag="VN178_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HWS81052.1"
                     /translation="MCRWLAYSGSPILMEELLYAPEHSLIDQSLHARLGVETTNGDGF
                     GLGWYAPHTETPAVFHSIEPAWNNRNLREVASHVQSPLFLAHIRASTGTAVQQTNCHP
                     FRYGRWLWVHNGLVRDFHRMKRDLALAVDDSLYASIEGSTDSELMFYLALTFGLEDDP
                     PRAVERMVGHVEEVGRRHGTEHPIQMTIGTTDGNSVWGFRYSSEGKSRTLYYSTDVRT
                     LRELYPERPRLREVSDEARIIVSEPLLDLPGAWNEVPESSYGVVREGDDELRPFRPVA
                     "
     gene            complement(11923..12198)
                     /locus_tag="VN178_08510"
     CDS             complement(11923..12198)
                     /locus_tag="VN178_08510"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWS81053.1"
                     /translation="MGLVAARAAGRMQARQTYRTMARMERRRSFVQSRMGMRQDFGRQ
                     EQPVEAAAPAPSEPEYVGELERLSQLRDQGIISNEDFEAKKKQILGI"
     gene            complement(12243..12776)
                     /locus_tag="VN178_08515"
     CDS             complement(12243..12776)
                     /locus_tag="VN178_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81054.1"
                     /translation="MTEKIGPVQLVAIGFPPDAEFEGHIMDELERLERHETIRILDLL
                     FVSKDAETGDLLALDYQGEELGAIVGALLGFEFDDNGGRPDESLAGMQGHAFGLSQSE
                     IHDIAASLEPGTSAGFLLIEHVWARDLKGAIRDAGGFPLGEGFLTPEALAEIAIELVA
                     MSEAIEELEAEEAGAGT"
     gene            complement(12861..13148)
                     /locus_tag="VN178_08520"
     CDS             complement(12861..13148)
                     /locus_tag="VN178_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81055.1"
                     /translation="MGCLFALFGGLFPRLALLILWIARPALVGAAFNTFLLPLLGIIF
                     LPFATLIYVIVYTPGVGLTGFDWFWVILAVLLDIGHWSASYTQRDQIPGVR"
     gene            complement(13309..13869)
                     /locus_tag="VN178_08525"
     CDS             complement(13309..13869)
                     /locus_tag="VN178_08525"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HWS81056.1"
                     /translation="MSNEGRYRQRLSTIAANWRAVALRGLVALLFGLVVLFWPGLVFA
                     VLALLFGLYAVVDGAITFWPALRSSEQSTRRWLPLGEGAVGIIAGLLALLWPGLTTNG
                     LVYVIVGWAVVTGTLKILTAILLRAEVENGWLLAGSGALSVLLGILLAALAGADVPFL
                     APLVGAFAVVVGLALIVFAFRLRERE"
     gene            13917..>14011
                     /locus_tag="VN178_08530"
     CDS             13917..>14011
                     /locus_tag="VN178_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HWS81057.1"
                     /translation="MAKELVSDELWNTIELLLPEDPPKPKGGRPRV"
BASE COUNT         2632 a         4380 c         4487 g         2512 t
ORIGIN      
        1 gaggggaatc tccttgagca actcctgtct ctgctcggga tgttcgtcga ggttcttgtt
       61 gatcggggtc tcgatggcgc cgggggcgat gttgtttacg gtgatgccgt gcggggccag
      121 ctcgactgcg atggtccgca tcagcatcct gacgccccct ttggccgcac agtagggaga
      181 gttcgtcggc atgggcaggt cctcgtgcac cgaggagacg ttgatgatgc gccccccatc
      241 tccctgggag accatctggc gggcggcctc ctggcagccg agccacaccc ccatgaggtt
      301 gaccgagatc accttctccc acacttctag gggcgtctcc agaaagggga tcttctgttc
      361 tatgcccgcg ttattgacca tgacgtcgag gcggccgaac tcgttgacgg tctgctggat
      421 aaggcgcttc acaccatcgg gctcggagac gtcggcctgt acggagatgg ccttgccccc
      481 gtttttctct atctcctcga ccgcttcctg tccttcttct gggtggcttc tgtagttgat
      541 ggccaccgag gccccttccc gcgcgaaggc gagggcgata gccctgccga tcccgaggga
      601 agagcccgtt accgcggcga ccttattctc tagttgcact gctaacctcc ctctcggata
      661 gtcggagcgc gcagttgacc agcgccaggt gtgtaaacgc ctgtgggaag ttgcccacca
      721 tcctctgtgc gcgcgtgtcg tactcttcgc tgagtagccc cacatcatta cgcagcgcga
      781 ggaggcgatc gaagagttcc tcggcctcgc gctttcggcc tatgccccaa agcgcgtaga
      841 ccatccagaa gctgcagatc aaaaacgagc cctcgcgccc tgtgaggccg tcgtcggatt
      901 ggtctgttcg gtagcgaagt acgaggccgt cttcggtcag ctcgctctgg atcgtctcga
      961 cggtactgat tacccgctcg tcgtcgtagg gcagaaaccc catgcgtggg atgagcagcg
     1021 cgcacgcgtc gagcgccgtg gaaccgtaag cctgggtgaa tgtcccccga ctggcgtcgt
     1081 agcctttctc caggacgtcg gcgtggatct cgtcccgcac ggcccgccag cgctccacgg
     1141 gaccatcgag cccgaaacgc tccacgccct tgaccagaga gtcgaacgcg acccagcaca
     1201 tgaccttgga gtagacgaag tgctgcatct ccccgcgcga ctcccagatc ccgttgtcgg
     1261 gcctcgtcca ggcctcttcc aggcgccctg cgagccagcg ctgcagttcc caacccctct
     1321 ccacagggac gatccccgcc gtccgaccca cgtgcgcggc cccgatcacc tcacctggca
     1381 catctccctg gacctggccc gcggcgtcgt tgccgacacg gaccggcgtg gcgccttcgt
     1441 ggccggggag ccagggcagt tcgtactcgg gcaggcggcg ggcgccgtcg atgccgtaca
     1501 tgatctgcgc gtcggcgggg tcgccggcga cggcccttag cacccattgc tgccaggcgc
     1561 gggcctcctc cagcaggcct gctcctatca gcgcgccgag ggtgaatgag ctatcgcgca
     1621 gccaacagta acggtagtcc cagttcctgg agccgccaag ggtctcgggg agcgaggtcg
     1681 tggcagcggc gacgatgccg ccgctcggcc tgtaggtcag ccccttgagg gtaaggaggg
     1741 accggtacac ggcgtcctgg ttgcgacctt cgtagctgat gccgccggcc caatcgagcc
     1801 agaagcgttc cgtgtcgtcc actgcctgca gaccgtcgat ggccacagga cgctcaccga
     1861 aagaaggcgc ccaggtgagt acgaaaggga cgcgctctcc ctcagagagc gtgaaccggg
     1921 agaggatctc accgcccacc tcctcgtggg gggccgatgc gcgcagccac agtgcgtccg
     1981 ggccggctac cgcacgcacc tcacgacctt ctgtggtgac ccatgggacg acgtgcccgt
     2041 agtcgaagcg tatcctcagc ctactcgtga ccgaaactct gccccttacg ccctcgacga
     2101 ccctgaccag gtcgggcgcc acaccgcggg gaggcataca atcgatcacc ctcatggcgc
     2161 cggtcggagt ctcccactcg gtctccagga cgagcgtgtg tcccctgtag cggcgcctcg
     2221 tgcagcggcc gcccgaggtg ggggccagac gccagcggcc gtgagagtcg ttgccgagca
     2281 gcgcggcgaa gcaggccggc gaatcgaagc gggggaagca cagccagtcc acagagccgt
     2341 cggagccgac cagggccgcg gtctgcaggt ctcccaccat cgcgtagtct tcgatcctcc
     2401 ctggcatcag accctactcc gtatcccagt actcttattc gtcaggatca tggccaatga
     2461 actatgccag agcctgagat atgcggccac cgtttcaggt tcacgaagtg caagggattg
     2521 ccgcattacg attgctgatc ttggccccgc ggcgcgcgtc tcttgtagac taggtgcgtt
     2581 agcgagcaca cgctgaggag agactcttgg tagggaacgg caccgccccg acgttcgggt
     2641 tgtggtacga cttcaggcaa aggttaccgc tcggcgacta cgcgcggttc tatgccgagt
     2701 gtctggagga gatcgaagag ggagagaggc ttggttttgc gggaatctgg ctctccgagc
     2761 accatttcgt ggacgacggg tacctgccct cgccgctcgt ggccgccgcc gcgatagccg
     2821 ccaggaccag gaccatgacc atcgggacga acgtgctcct gttgccgatg caccatcctc
     2881 tgagggtggc tgaggatgcg gcggtagtcg acctcatctc aggcgggcgg ttcgtgctag
     2941 gggtcggcca gggctacgtg cagcacgagt tcgagacgct cggcttcgag cggaagtaca
     3001 ggccctcgct cttcgaggag gggatcgagg tgatccgtca cgccctcgga gaggggagga
     3061 cgggctacga aggtagacgc tggagattcg acgacctgcc tttcggcccc cgccccgatg
     3121 gcaggctccc cttgtatatc ggagcttttg cggacccggc catagaccgc gccgcacgcc
     3181 tcgccgacgg gttcctggct tccgcgggcg ggggcgcctt cggcgagacg cacaggaagg
     3241 tcctggcggc gctggagcgg cacgggcgcg ccggagaggc gttcccttac gtggcctcgg
     3301 gagtgctctt cgtggatgag gatgctgggc gggccaggga gatcgtcggt cccgccatcg
     3361 cctaccagcg cacgcgctac gccgaatggg gtaccgacag ggacaagccg cgtccgggac
     3421 cgatcaggga ggaggatcta ccctgggaga ggtatctggt cggcacgcca gaggaggttg
     3481 cgcaagcgtt gatcgagctg taccaggagg cgccttacga ccacctctgc ttctggggac
     3541 gtctccctgg tgtcacccac gaacaggccc tggccaacat gcgcctgttc gcctccacag
     3601 tcgtcccgcg cgtccgcgac gcggtcgagg tttgagggcg atcccgggcg tactggacga
     3661 ggatcggacc gccttcctcg tgcgccagag ggtggcccgg ctcgctacct cagatgggat
     3721 gggcaaaccg cacgccgtgc ccgtgtgctt cgccatggcg cgggcttcga tcttcatagc
     3781 cctcgacgag aagccgaaag acgtacccgt aacccgactc aaacgcgtca ggaacatcct
     3841 cgagaacccc gaagtagcgc tcatcgcgga caggtacgcc gaggactggg aactgcttgc
     3901 tttcgtaatg gtgcgcggca gggccgagct tctggacccc ggcaacgagg agcacaccgc
     3961 cgccgtgagg ctcctgcggg gcaagtacca ccagtacgag gcgatgcgca tagaggagaa
     4021 ccccatcatc gccatacgtc ccgagagggc tgcctcctgg ggcgccctcg acgcgccgga
     4081 gtcctctgag ccccagatcc tcgacacgat ccacggccgc cgctccgtca ggaagtatct
     4141 cgagaaggaa gtgtccgagg accttgtcga gcgggtactc gaagccgcca ggtgggcacc
     4201 atctccccac ggacgacaac cctggcgatt cgccgtgttc accaaagagg cgaccaaaga
     4261 gcgtctggcc cgcgcgatgg gcgaggagtg gagggcgaat ctggagatgg acggccagag
     4321 cgtagagatc gtagagaagc gtctcgaagg ctcccgcagg cgcctgctcg aggcgcctgt
     4381 attggtgttg atctgcctct acctcgaaga cctcgacgcc tacccagatg cggagcgcca
     4441 gtgcggcgag aggacgatgg ccgtacagtc tttaggagct gccgcccaga acgcgctgct
     4501 cgctgcctat gactcgggcc tcgacgcggg ctggatgtgc gcgccactct tctgccccga
     4561 gagggtggtc gaggcgcttg gtctcgatcc gagcctggtt ccgcacgccc tgctcacgct
     4621 ggggtacgcc gagggggacc ccccgaagcg tcggggacgg aagtccctcg acgaactcgt
     4681 cgtttacagg gactgacggc gccgggcatg aggcttcttt accgtaagcg cgacaaacat
     4741 ttcggtactt cggctttgtt ggagaggcac ctgatttggt cgtgctctat ctgtggcata
     4801 tagagttccg catacgaacg taaactcgat aaactttgtc ggatatacgc tagaatgtgc
     4861 gcttgtgtgg tgtagacgga gttaggagaa cccatgacgg agcaggagat acaggagaaa
     4921 ggttatgcga accccgacgc ccttgttagt acccagtggg tcgcagagaa cctggacgac
     4981 acggagaacg tacgcatagt cgaatcggac gaggacgtgc tcctctacga cataggccat
     5041 gtccctaacg ccgtgaagat agactgggtc gaggacttga acgatccctt gatgcgcgac
     5101 tacctcgacc cggagcactt cgcctccctc atggccgaga agggcatctc tcccgatacg
     5161 acggtcgtct tttacgggga taggaacaac tggtgggcca cgtacgccct gtgggtcttc
     5221 cgcctgttcg gccatgagaa cgtcaaggtg atggacggag gaagggtcaa gtgggaggcc
     5281 gaaggcaggg agatgtccca ggagacgcct tcctttccta acgcccagta ccccaccccg
     5341 cagcgggatg actcgaagat cagggccttc cgcgaagacg taatggcgca tctcgagaag
     5401 gtccgctcct ccgagggagg acttatcgac gtgagaagtc ccggggagta cagaggagag
     5461 ttgctgcaca tgcccgacta cccccaggaa ggagccctga ggggggggca tatcccggga
     5521 gcggcgaacg tgccgtgggc gagagcggcc aacgaagacg gcaccttcaa gaatgcgcag
     5581 gaactgcgcg agatctacga aggagaggca ggtctcagcc ccgatgagga cacgatcgcc
     5641 tactgccgca taggggagcg ctcctcccac acctggttcg tgctctcgta tctgctcggg
     5701 tacgacaacg taaagaacta tgatggctcg tggaccgagt ggggcaacac cgttcgggct
     5761 cccatagaaa gatagcaatt tggcagcgaa gccaggggcg tcggatcgca aaaggaatgg
     5821 tgccgcgctc ctggtcgagc acgccaaaaa tcctcgccac aagaacgtgg agggcgtaca
     5881 agaagaagcc gacgtcgtcg taccaggcgg cagccccgag tgtggggggt cggtggtcgt
     5941 ctacctcaag gggggcgacg acggcaagat agagcggctc tcctggacgg gtcagggcga
     6001 caccatcagc atgggagcca cctccatagt gctcgagcgc atcctggacg agggcctcag
     6061 catggaagag atcctcgaga tggactacga ggagttcgtc gattccctgg gtagagaggt
     6121 tataggtagc cgcaggaggc atgctacgct ggggctctcc accataaagg gcgctataag
     6181 gatctatcag aggaagagcc ggtcggcagt caaggagcga gccggaggct aggttccgtg
     6241 ttggcagccg tcgtcggagc ctttcagcat agacgtcacg cccggtccag ctattagagc
     6301 cgggagggga aagccgagat ccaggcgctc tacggcgaaa cgctgcacgg tctgcgctac
     6361 gagagcctga cttttcggga tagatcgacc cgaagtccgt ccggcggata ggctgccacc
     6421 gaaaggcacg tgcgggtgct gcaggccacg ctccgctaaa ctctacgttc gtgagcgatg
     6481 caggcgagaa cacatctggg cggctgtggg gaggtaggtt cggagcctca cccgcagagg
     6541 ccttcgagcg cctcaacgcc tccatcccct tcgacatccg tctagctcct cacgatgtac
     6601 gtggctccat tgcccacgcg aggatgctgg gggtgcaagg gatcatctcc gatggcgagt
     6661 cagcgatgct ggtaagtggg ctcgaagcgg tgctgcggga ggttgaggcg ggggagttca
     6721 cgtgggatct ctcggacgag gatgtgcaca cggcggtaga gcgcaggctg aaggagctcg
     6781 tgggcgacgt ggcgctcaag ctgcataccg gccgcagccg caacgatcag gtagccctcg
     6841 atctgcacct cttcgccagg gacgctgcag aacggatcag ctcggacata ctcgccgcga
     6901 tgcgggcact cctcggggtg gccgagacga acaaggatct cattctaccc ggatacaccc
     6961 atcttcagcg cgcgcagccg atcctgctct cccaccacct gctcgcccaa ttcgaggcgt
     7021 tgcgccggga cctccgaagg ttcgaggccg ccagggaggc ggcgaacgtc tcgccgctgg
     7081 gggccgcggc gctcggcggc acgccgcatc ccgtagatcc caggctcacg gcggaggagc
     7141 tcgggatgga gccgttcacc aactcgctgg atgcggtctc ggagcgggat ttcgcccttg
     7201 acctcctcta cgcctgcgcc gtactcgggg tgcacctgag caggctcggg gaggaatggg
     7261 tgctttggac cagcgccgag ttcgggttcg ccaccttgga cgacgcttac tcttcgggtt
     7321 cgtccattat gccccagaag aagaaccccg acgcctacga actcatgcgt ggtaagacag
     7381 gccgtctgat cggggatttg aacgcggtcc tgatcaccct caagggactc ccactcggct
     7441 actcgaaaga cctccaagag gataaagagc ctctcttcga cgcggtcgac tctatgctcg
     7501 ccatgctcgc cattctgccc gagatgctgc gaacggcgcg cttccacgga atacggatgg
     7561 ccgaggctgc ggggggtttc gccctcgcca cggagctggc tgacttcctc gcggcagggg
     7621 acgttccttt cagagaggcg catcacgccg tggggaggct cgtgcggagg tgcgaggagc
     7681 tgggagtgtc gctggaggac gtgccgcaag tcgagctcgc ggcggcgcat cccgcgctct
     7741 cggaggtgcc agaaggactg cttacgccgc gcgggagcgt gcgcaacaag cggtcggccg
     7801 gctccacggc cccggcttcc gtcgatgagc agctcaggca ggcgaaagcg tttctggaga
     7861 cggtggaaag ccactcgaaa ggccaccttt cggaagggag tgacagatgc ggttagtaca
     7921 gatgtccgac gtccacgtgg gttctggact gtttcggccg gacctcctgg aggccactat
     7981 cgaggagagc aacgccctgg agccggacct cgtggccatc gtgggggacc ttacgatgga
     8041 gggataccgc tgggagttcg aggaggcgag agagtttttc gaccgacttg agtgtccgaa
     8101 cgtcctgtac gcgatgggca accacgacgc caagaacgtt ggctacaggc atttcgagga
     8161 gttcttcggc ataagggaga ggtcggcccg gatccccgtc cccgaaggag aggccaaggt
     8221 cgtcgtgctt gactctacca agccggatct cgacgagggg gaggtcgggc gcgagcacta
     8281 cagctggctc gactccgagt tccggggctg ggatcgtgga ccgaggatac tcatgatcca
     8341 ccaccacatc ctcgccgttc ccggcacggg acgcgacgtc aacaacctgc gcgacgcggg
     8401 cgacgtgatg gccatcctgc gcgaactgaa agtcgatatg gtcctctcgg gccacaggca
     8461 cgtcccttac gtctggagca tctcgggggt gaggatcgtc cactcgggca ccgtatcgtc
     8521 catgcgcgta cgcggcacga tgccgccttc ttacaacgtc atcgagatcg acgaacagaa
     8581 cgtcagcatc accctgcacc agcctggcat gggcgaggcg ggccgcgagc ccctcgcctc
     8641 cttctcccgt actcccgtga ccacgagcga gttctacccg gacttcgagc gcttcgtcct
     8701 ctacgaccgg ctgccgttcc taaagtgaag cacacgtacc gtgagctggt accctgaagg
     8761 accgagatca ggaggagaca ctgtgtcgac cgaagagaac aatcgtctgg ccagggaagc
     8821 cattcgcatc tggaccacag gagatctggg tgccgccgat gacgtctacg ctcccgacta
     8881 cataaaccat caacaccacg accctgagga cccccgtgat ctccatggtg tggaggctga
     8941 agtccttcgt ggcggagttt cgcagtgcct tcccggactt ccacgactcg atcgacatcc
     9001 aggtcgccga aggtgaactg gtagtcaccc gcttcacgtc catgggtacg caccggggca
     9061 cgtttatggg tgtcgagccc acgaacaggg aactcgtctg gaccgggata accatcgacc
     9121 gcgtctccgg gggcaagata gtggagagat gggccaactg ggatatgatg ggcatgatgc
     9181 agcagctcgg cgccatctca tcatccggag agatctccga agaagcaagt cctacttaag
     9241 cgtacaggcg attcctcgac aatagatctc ttgccgggcg actacagaaa ttcttcagcc
     9301 tcgctgagct ggcccgtctt ctcgaaggcc tgacgcaaga gcaagcgtcc ctcttcgcgt
     9361 tcctcctctg tacgggcttt ctccagcacg gtctttacgg attcctcgat ggcttcgggc
     9421 tcgaaagtga tatccggcag ggcgatgacg acttccctga tctggcggta gataaagtcg
     9481 ggttccccag cgtataccca cccgcggcgg gcgaggaccc aggcccagcc tttcatacgg
     9541 atggtgttct ctatgtcctc gtcttcgaga ccgaacatct cgatcctgag cctgtgtgcc
     9601 gttattagct ccggctttat gcgtactgtc gttggcatgg aaacagttta acagcgacac
     9661 tcgggccaac gatcaaagga cccgccccga ctttatcttc gccgcgaacc tgtccccacc
     9721 gagatgatag gcgatatgat gccaggctgg agcatcgagg aggagcacat ctgctttgta
     9781 gcctggcgcg agcctgccga cgtcggcggc gccgaggaca tgagccgcgt tggccgtgca
     9841 ggcgcagagg gcttctgccg gagagaaccc gaggcgggtg cacgccaggt tcatcaccag
     9901 cggcagcgac tcgcagaatg agcttccagg gttgaagtct gtggctagcg ctacgatcgc
     9961 tccctcgtcg gccaggagcc gtgccggagg gaggggcagg tcgaggaaga gcccgcaagc
    10021 ggggaggagt acggccgcga cgtcgctcct cgccagcagg ctggccgctt catgcttcgt
    10081 ggcttcgagg tggtctacgc tacgggcgcc gagctcgagg gccagcggga tcgcaccccg
    10141 ctcggagaac tggtcagcat gcaagcggag cgcaagcccg tactcttggc acgcttccag
    10201 gtagcgccgt gcttcaggca cctcgaaaga gccacgctcc aggaagatgt cggcggatcc
    10261 ggccaggggc gctgcttctg gcagcacttc attgatcacg aactctacgt actcggccgc
    10321 gctatcgaac tcgggcggga tcgtatgggc cccgaggaag gtaggatata cgtctacggg
    10381 actgcgcctt cctgcgtcct ggatggcacg cagggacttc agctccgtct cgcggtctag
    10441 accgtagccc gacttcgcct ctgcggtcgt cgtcccgtgc tccagcatcc agccgaggtg
    10501 acgctcgacg gaggaccgaa gatcgcgctc actaccggca cgtgtcgccg cgaccgtcga
    10561 gcggataccg ccaccggaag catggatctc ctcgtaggta gcccccgagg aacgcagctc
    10621 gaactccgcc gccctgtctc ccaggaaggc cgggtgcgtg tggcagtcta cgaggccagg
    10681 aagcgccacc ttcccacgcc catccacact ctcgtagtca acgccggatg gaaggtcatg
    10741 gcagatctcc gctggcgggc cgactgcggc gatgcgctca cccgcgatag ctatcgaggc
    10801 gggggagtga atgcgcagtg ctccgaaacc cctgccccgg agaggtcccg agcccgccgg
    10861 ggtgacggcg gcggcgaggt cgtggatcag taacgtggcc tcagccattc atgcgacagg
    10921 ccggaacggg cgcaactcgt catctccctc tcttacgacg ccgtagctcg attcaggcac
    10981 ctcgttccac gcccctggta ggtccagaag cggttctgag acgatgatcc gcgcctcgtc
    11041 tgagacctct cgaaggcgcg gccgctcagg gtagagctcc cgaagcgtcc tgacgtcggt
    11101 ggagtagtaa agcgtgcgag acttgccttc gctagagtac ctgaaccccc agacgctgtt
    11161 cccgtccgtg gtcccgatcg tcatctggat ggggtgctca gtgccgtggc ggcgtcccac
    11221 ctcctcgacg tgcccgacca tcctctccac cgcccgcggg ggatcgtcct ccaagccgaa
    11281 cgtgagggcc aggtagaaca tcaactccga atcggtcgag ccctcgatgg aggcgtaaag
    11341 cgagtcgtcc acggccagcg ccaggtcgcg tttcatgcga tggaagtcgc gcacgaggcc
    11401 gttgtggacc caaagccacc tgccgtaacg gaaagggtgg cagttggtct gttgcacggc
    11461 cgtgcccgta gaggctcgga tgtgggccaa gaacaaaggc gactgtacgt gggaggcgac
    11521 ctctcggagg ttgcggttgt tccaggccgg ctcgatggag tggaagacgg ccggggtctc
    11581 ggtatggggg gcgtaccagc cgaggccgaa gccgtcgccg tttgtggtct cgacaccgag
    11641 gcgggcgtgg aggctctggt cgataaggga gtgttctggt gcgtagagca gctcctccat
    11701 gaggatgggg gagcccgagt aggctagcca gcggcacatt ggttgctcct tcctttcgag
    11761 gtcggcttcc gccagcctct tgccttacgg ctaatggacc aggggtgctg gcatcgatga
    11821 agaactcgcc tagttggcgc caggaagcga cccacctcgg cccgggacga tgcccgtcct
    11881 tccacggtgg gtctctcctg gcatcactcg acgcgcgttc tttcagattc ccaggatctg
    11941 cttcttcttg gcctcgaagt cctcgttgct gatgatgccc tgatcccgaa gctgggagag
    12001 ccgttcgagc tcacccacgt attcgggctc ggacggggca ggggccgcgg cttcgaccgg
    12061 ctgttcctgg cggccaaagt cctgcctcat tcccatcctg ctctggacga acgagcggcg
    12121 ccgctccatg cgcgccatcg tccggtaggt ctgccttgcc tgcatacggc ctgcggctcg
    12181 tgccgcgacg agtcccatct atcttctcct ttcctgcgtc atttggttcg gcttgctctt
    12241 ccctacgtcc cggcgcccgc ctcctcggcc tccagttcct cgatggcctc ggacatcgcg
    12301 acgagctcga tagcgatctc ggcgagggcc tctggagtga ggaacccctc cccgagtggg
    12361 aaaccgcccg catcgcggat ggcgcccttc agatcgcgcg cccatacgtg ctcgatgaga
    12421 aggaagcctg cggaggtgcc cggctccagc gaggcggcaa tgtcgtgtat ctcgctctgc
    12481 gagagcccga aggcgtggcc ctgcattcct gccaacgatt cgtctggtcg tcctccgtta
    12541 tcgtcgaact cgaatccgag cagcgcaccg acgatcgcgc ccaactcctc gccctggtag
    12601 tccagcgcca gtagatcccc cgtctcggca tccttgctta cgaacaaaag gtccaggatg
    12661 cgaatggtct cgtggcgctc tagtctttcg agttcgtcca tgatgtggcc ctcgaactcc
    12721 gcatccgggg gaaagccgat cgccacgagc tgaaccggcc ctattttctc cgtcatcgtt
    12781 cacctccatc tgttgtccgc cgagtcacgc ttttatcccg cctccatgcc cgggtagatc
    12841 tctgccatga ttccccgcgt tcaccgaacc ccggggatct ggtcgcgttg ggtgtagctg
    12901 gccgaccagt ggccgatgtc gagcaggacg gcgaggatca cccagaacca atcgaagccc
    12961 gtgaggccca ctccgggggt gtagacgata acgtagatca gggtcgcaaa cgggaggaag
    13021 atgatgccaa gcagcggcag gagaaacgtg ttgaacgctg caccgacgag ggccggtctg
    13081 gcgatccaga gtatgagcag cgcgaggcgc gggaagagac ctccgaatag cgcgaaaaga
    13141 caacccatcc tgtttacctc ccgtcaggtg tttgctcctg ctctgcacga cgtctctcga
    13201 gaataagccc gagtgaagtg gtgggtcatc acccaggcac gcatatcccc taggggaatt
    13261 cggcacccgc tggtagaacc acctgtagca gcggctccca ttagttcatc attcccgctc
    13321 ccgcaaacgg aatgcgaaca ctatcaaagc taggccaacc accaccgcga aggcaccgac
    13381 gagcggcgcc aggaacggca catctgcgcc ggccagggct gcaaggagta tgccgaggag
    13441 caccgacagg gcgccgcttc cggccagcag ccacccattc tccacctctg cacgcaaaag
    13501 aattgcggtg agtattttga gcgtaccggt caccaccgcc cacccgacga tgacgtagac
    13561 caggccgtta gtggtcagtc cgggccagag gagggctagc aatccggcga taatacccac
    13621 cgccccttca cccagcggga gccaccttcg cgtgctttgt tccgaagatc tgagagcggg
    13681 ccagaaggtg atcgccccgt cgaccaccgc gtagaggccg aacaggaggg cgagcacggc
    13741 gaagacgagg ccaggccaga agaggaccac gagaccgaat agcagggcga cgagaccacg
    13801 caatgccacg gctcgccagt tggcggcgat cgtgctcaac ctctgcctgt agcgtccttc
    13861 gttgctcatg actaagtgat cctttcaaaa cttgctgatg cgggtaagga aaagatatgg
    13921 ctaaggaact cgtatccgac gaactgtgga acactataga attgttgttg cccgaagatc
    13981 ctcccaaacc caaaggcggt agacctcgag t
//
LOCUS       DATMVF010000171        12053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_348843, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000171 DATMVF010000000
VERSION     DATMVF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12053
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348843"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VN178_08535"
     CDS             <1..645
                     /locus_tag="VN178_08535"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HWS81058.1"
                     /translation="TEHDRYLEEIDLAAFQEARELSQDVDLRLGIEIDYVPGAEERME
                     NDATALPYDYVIGSVHRVDDEEVDRATDQRIYDRYETYDLYAAYYANVSKAALSGRFE
                     ILGHPDLIKIFRHFPDRDITPILEETADAVAESGIVIDVNSAGLRKPVGEVYPAREFL
                     RMFHKRGVPIVLSSDAHAPDQVAMGYDTSLALVHDVGYREVVTFRNRERGTLPL"
     gene            873..1583
                     /locus_tag="VN178_08540"
     CDS             873..1583
                     /locus_tag="VN178_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HWS81059.1"
                     /translation="MTEELFLTDSYTKEFDARVVKLDGREVILDRTAFYPGGGGQPPD
                     KGSLGIGPIEAMVVDVRREGGGIVHVMDRPIPDTVQELKAELDWGRRFAHMQYHTALH
                     VLSGVIWRNFDARVTGGQMRADRARMDFSFPGEWTVDIVGEIERLTNGALAEERPVRV
                     YELEREKALENPDLIRTQVNLVPERVRRIRIVEIQGLDTQADGGTHVANTREVGRMEI
                     TGHKSKGRQNKRVEFVLR"
     gene            1580..2104
                     /locus_tag="VN178_08545"
     CDS             1580..2104
                     /locus_tag="VN178_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWS81060.1"
                     /translation="MRFRRIVVVFALLLVGCGDPQADPDTQSQRTSQASDTTAVGREE
                     TTSGPSGLRTLVIDASGGKEVEVRVEVADDVAEQAKGLMDRTTLGENRGMLFVFPEER
                     ELSFWMKNTLIPLSIAFIDSESRIIDIQDMKPLDDEPPHYVSAEPAQYTLEVNRGFFE
                     ERGVRVGDRVEMPE"
     gene            complement(2111..3247)
                     /locus_tag="VN178_08550"
     CDS             complement(2111..3247)
                     /locus_tag="VN178_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81061.1"
                     /translation="MVSERKITDLPDLISDLSPEDRILANRIFDVSTTTGRLAPPEAM
                     HAWIEKSFGSVDHVTAQRIVKVTNRITLEGTLFNELRASRPLDTGVSLDLDREIAATE
                     GDPFCHPKEGTPADVFGRVEGDHAISASNVAKYDGFHGVIVFDYHNPLHLTPEKVSGY
                     VSVGLEWGLKALQADPEACYPFLMWNCLWRAGGSIIHGHAQVTATRGSHYPKVERLRR
                     NASAYKLEHGSDYFEDLHGIHASLGLDVPLEGTRAFASLSPIKEKELVVIGSEPGDES
                     LGHAVGALLQGYVEALGVRAFNVAFYMPPLAPTQEDWTDFPTVVYIVDRGDPSNRTSD
                     IGAMELYAASVVASDPFRVAEALRERRSDYTVGAKRPAVDKGAR"
     gene            complement(3251..5758)
                     /locus_tag="VN178_08555"
     CDS             complement(3251..5758)
                     /locus_tag="VN178_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWS81062.1"
                     /translation="MHVAFINPQGNFDPDDSYWTAHPDFGGQLVYVKELALAMGNLGH
                     KADIVTRRIVDPEWPEFASDTDAYPDAPNVRILRIPCGPDTFLAKEELWPYLGTEFVP
                     RLLELYENQGSMPDVVTAHYGDGGLCAALIEERTGIPFSFTAHSLGAQKLDKMGIPRS
                     DIAEVDARYHFSRRIVAERLAMNRSNVNVTSTSQERFNQYTHNAYRGAVDPTDDERFA
                     AVPPGVALHIFDKSARYQEEEAVREYVRASLERDLSPDRAGLPCVVASSRLDPKKNHL
                     ALVEAFAGSPTLRESANLVILTGSLDDPLEDYGAAKDEERAVLDSLMEVIDRARMRGE
                     VSMFAVRGQRALAATYRYLAERRSVFALTALYEPFGLAPLEAMAAGLPAVVTKFGGPS
                     ESMREGDEEYGILVDPTDPEEVGAGLHTLTGSQELWELYARAGYGRVLARYTWEKTAE
                     GYLEAISGRSDAVRGREEDATTLHAGDDQGVISEARSPARLEIPPYFTDPASGDGVSL
                     EGLDELYYRLDLLAVGETMVDFISHEMANSLRTAGQFSRYLGGQPANVAVYVAKLGGR
                     SAIITKLGSDYFGEYVEDQLQHHGVNTEAVSRTNEASTTNAFVTRTVTVPDFQVNRGA
                     DALLAVRQVDEEMISRARIVHTSAFALSKDPQRLAVRRAMRLGSRLGKIVTLDPNYDP
                     RVWPDKVEAWEVLAEVMPYVTIVKPSLEDARHLFDVNMDEETLEETAISEFHDLGAKV
                     VILTRSGGVVTVSDNGNLERVGPLPEVEVSHVTGASDAFWSALLMARLDGKDWQEAVR
                     FAHEVASLKLRAEGHVERMIDREGLYAGLKSAGERTP"
     gene            complement(5763..6743)
                     /locus_tag="VN178_08560"
     CDS             complement(5763..6743)
                     /locus_tag="VN178_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HWS81063.1"
                     /translation="MAPETEPLDVLTIGEMVVDFISVEKTDTLSNASTFERHLGGSPA
                     NIAVYVSKLGGTSAVIAKTGIGAFGQFLKSELQRHGVTTDYLLMDHRTHTTVIFISST
                     AATPDFEEFRSGDSLLRPQEISEEAISRARIVHASTFALSLEPCRSAVLEAFRKAREM
                     GKIVSLDPNYSRRVWPDYKEARKVIRDAYRHVNVTKPSAVDARRVFGPDYQPEQYIEM
                     FHDLGAETVIFTMGEEGNLISENGRITTHVPARPVEVVDVTGAGDAFWAGFLTATLDG
                     NSAERSVLFAREVVERKLTTKGPLPSEIEREELYAGLRRSQGAKSDGQKG"
     gene            6892..7761
                     /locus_tag="VN178_08565"
     CDS             6892..7761
                     /locus_tag="VN178_08565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS81064.1"
                     /translation="MDYSNGGMGGKTVLITGGTSGIGKAAAVALAAMGANVVVVGRNR
                     ERGEAAIEEISARSHNESVELLLADLSVQSEVRGFAQEFQERHDRIDVLVNNAGLVQS
                     KRTETPDGIETTLAINHLAPFLLTNLLLDRLKGSAPSRVLTVSSEAQRWGTMDFDDLQ
                     SRRKYRGFPVYGMTKLANIMFTYELAERLNGTGVSANCLHPGSVGTNFGQNNRGPMAL
                     FFRTFKPFMRSPERGADTLVWLASSPEVEGVSGKYYFDRKEIEAKKVAYDPAARRRLW
                     EISEELTGLKVAA"
     gene            7761..8750
                     /locus_tag="VN178_08570"
     CDS             7761..8750
                     /locus_tag="VN178_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS81065.1"
                     /translation="MGRERGIEVQNLVRKFRKGPRAVDGINLRVEPGEVYGFLGPNGA
                     GKSTTVLMLTTLLPPTSGTATVAGYDIVHEGPQVRAFIGAALQEAALDPFLTGREHLR
                     LQAALHGLSRKERKRRGQELLERVGLEEAADRKVRGYSGGMKRRLDLALALVHGPQIL
                     FLDEPTTGLDPQSRSALWAEVSRLARDEGVTVFLTTQYLEEADVLADRVGIIDRGKIV
                     AEDTPEALKAEIGRPSVEATPAERSERDAVARVLREFGEEIPAQPGAVAVRLSSGAAG
                     LADVVRALDRGELRVANLRLDEPSLDDVFLAKTGRSLEGAGEDEEGEMDEVHA"
     gene            8747..9523
                     /locus_tag="VN178_08575"
     CDS             8747..9523
                     /locus_tag="VN178_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS81066.1"
                     /translation="MRFSTFLTEVMAIARRSTVRTLRQPAVIVPSLLFPMLFFSINAN
                     GLDAASKIPGFPADSYLDFAFAFPLIQASLFGAITAGADLARDIESGFFDRLSLTPMR
                     PVALLGGMLTGVVALGFLQGIVFLTIGSLMGVDVSSGFLGMVVIVLLTVLVALGFGGI
                     GAILAIKTGSVEAVESAFPLFFVAIFLSSINLPRDLIEQDWFRFIATINPISYLVEGI
                     RSLVITGWDPQALLLGFGFALAIVVVAIGGAAASLKTRVA"
     gene            9526..10290
                     /locus_tag="VN178_08580"
     CDS             9526..10290
                     /locus_tag="VN178_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS81067.1"
                     /translation="MGGFFDVAFAVAGRQIYKYFTNPAFLAPALFPLFFFVAFAGGLT
                     RVADVPGFDYTAGYIAFQYVWALLQAVSMGGAFTGFSIAGDFENGFARRLMLAASNRY
                     GIILGYTIASIVRAAMTGILVTVIALVTGMPISGGYDFFGLVALALLANIAATLFGAG
                     VAMLLRTQQAGPVIRTPIFLVLFLAPVFVPLKLLSGWLEAVARLNPFTAILEAARGFL
                     AGDPTGVASAFIISAVLILVLALWAVYGLRRAERAG"
     gene            10305..10787
                     /locus_tag="VN178_08585"
     CDS             10305..10787
                     /locus_tag="VN178_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWS81068.1"
                     /translation="MTASEGKRTTEKDLSGPARLALKLGSVAHAGVYRATGGKVFGRM
                     GKGPILLLNTVGRKSGKKRTSPLLYVMDGEDFVVIASKGGAATHPAWYLNLRANPEAT
                     VEIGDREVQVEAEVADPEDKARLWQKMVEMYPAYDDYQKKTEREIPLLILRPVEGRPS
                     "
     gene            10784..10984
                     /locus_tag="VN178_08590"
     CDS             10784..10984
                     /locus_tag="VN178_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWS81069.1"
                     /translation="MKTISREDLREKIDRGDDFVLLEALSEASYERVHLPGATRFQDM
                     DSIPDLLPDKTTEVVAYCSSFD"
     gene            11153..11243
                     /locus_tag="VN178_08595"
     tRNA            11153..11243
                     /locus_tag="VN178_08595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11188..11190,aa:Ser,seq:gga)
     gene            complement(11812..>12053)
                     /locus_tag="VN178_08600"
     CDS             complement(11812..>12053)
                     /locus_tag="VN178_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81070.1"
                     /translation="GWLALTTGVTLAYQRLQITGNVADVPDPDDRGGPGAERAAALLL
                     LSYYLPAALWALKQERRKYLLYSVPFATMLCAALK"
BASE COUNT         2154 a         3622 c         3996 g         2281 t
ORIGIN      
        1 accgagcacg accgctacct cgaagagata gacctcgccg cttttcagga ggcccgtgag
       61 ctctcgcaag acgtagatct gcgactcggg atagagatag actacgtacc aggagcagag
      121 gaacggatgg aaaacgacgc cacggccctc ccctacgatt acgtgatcgg cagcgtacac
      181 agggtggacg acgaggaagt ggacagagcc acggaccaga ggatctacga caggtacgag
      241 acctacgacc tctacgcggc gtactacgcg aacgtcagca aggccgcgct ctcgggaagg
      301 ttcgagatcc tcggtcaccc tgacctcata aagatcttcc gccacttccc cgacagggac
      361 atcacaccga tcctcgagga aaccgcggac gccgtcgccg agtccggcat cgtcatcgac
      421 gtgaactcag ctggactcag gaagcccgtt ggcgaggtct atcctgcgag agaatttctc
      481 cgaatgttcc acaagcgagg cgttcctatc gtcctctcct cggatgccca cgccccggat
      541 caagtcgcca tgggctacga caccagcctc gccctcgtcc acgacgtcgg ctaccgcgag
      601 gtcgtaacct tcagaaaccg cgagcggggg acactcccac tttgatagca attcagcacg
      661 cctgcttcgc aggcttgtca gcatttcagc ttgtcagcac atcgggctgt agtaccctat
      721 cctagtgcgc gggagcgact ctaggagact cctccacgct tcaggccacg tccaccaacc
      781 ggctagagtg ttgagagtcg cgaagcggcg tgctgacgtg ctgacaagcg aagcccgaag
      841 ggctgagcgt gctgaaatgc tgaagtgctg aaatgaccga agagctcttt ctgacagact
      901 cctacacaaa ggagttcgac gcgcgcgtcg taaagctgga cggtcgtgag gtgatactcg
      961 accggacagc attctatcct ggcggcggag gccagccgcc ggacaagggg tctctcggca
     1021 tcggtcctat cgaggctatg gtcgtagatg tgaggcgaga aggcggcggg atcgtccacg
     1081 tcatggacag acctatccct gataccgtac aggaattgaa ggccgagctg gactgggggc
     1141 gtcgcttcgc ccacatgcaa taccacacgg cgctgcacgt cctctcgggt gtgatctggc
     1201 gcaacttcga cgccagggtc acgggcggcc agatgcgcgc ggaccgtgcc aggatggact
     1261 tctctttccc cggcgagtgg accgtcgaca tcgtcggtga gatcgaacgc ctgacgaacg
     1321 gggctctcgc cgaagaacgt cctgtaaggg tctacgagct ggagcgtgag aaggcgctcg
     1381 agaatcccga cctcatccgt acccaggtaa acctggtgcc cgagagggtg cggcgtattc
     1441 ggatcgtgga gatacagggc ctcgatacgc aggccgatgg cgggacccac gtcgccaaca
     1501 cgcgcgaagt cgggaggatg gagatcacgg gccacaagag caagggccgc cagaacaaac
     1561 gagtagagtt cgtgctccgg tgagattcag gaggatcgtt gtcgtctttg ccctgctcct
     1621 ggtcggttgc ggtgatcccc aggccgaccc cgacacacaa agccagagaa cctctcaggc
     1681 ttccgacacc accgctgtgg ggagagaaga gactacgtca ggaccatcgg ggctccgcac
     1741 gctggttatc gacgcttcgg gagggaagga ggtcgaggtg cgggtcgagg ttgccgatga
     1801 cgtggccgaa caggcgaagg gtctcatgga caggacgacg ctaggggaga acaggggcat
     1861 gctcttcgtc ttccccgaag agcgggaact ctctttctgg atgaagaaca ccctgatacc
     1921 gctctctatt gccttcatcg attcggagag tcgcatcata gacatccagg atatgaagcc
     1981 cctcgacgat gaacctcccc actacgtctc cgccgagccc gcccagtaca ccctcgaagt
     2041 gaaccggggc ttcttcgaag agcggggtgt aagggttgga gatcgcgtcg agatgccgga
     2101 ataggccttc tcaccgggcg cccttatcga ccgctggccg tttggcccct actgtgtagt
     2161 ctgaccttcg ctcgcgcaga gcttcggcga ctcggaaggg gtcggaggcg acgacggagg
     2221 ccgcgtagag ctccatcgct cctatatccg aggtacggtt ggacgggtcg ccccggtcca
     2281 cgatgtagac gaccgtcggg aagtccgtcc agtcctcctg cgtgggagcg agtgggggca
     2341 tgtagaaggc gacgttgaag gcgcggactc cgagggcttc gacgtaacct tgcagcaagg
     2401 cgccgaccgc gtggcccaga gattcgtctc cgggctcgct cccgatcacg acgagctctt
     2461 tctccttgat gggggagagg ctcgcgaacg cgcgtgtgcc ttccagcggg acgtccaggc
     2521 cgagagatgc gtggatgccg tggaggtcct cgaagtagtc ggagccgtgc tccagtttgt
     2581 aggccgaagc gttgcggcgg aggcgttcga ctttcgggta gtgggagcct cgggttgccg
     2641 tgacctgcgc gtgcccgtgg atgatggagc cgcctgcccg ccagaggcag ttccacatca
     2701 ggaaggggta gcacgcctcg gggtcggcct ggagggcctt aaggccccat tctaggccta
     2761 cagagacgta gcctgagacc ttctcgggcg ttaggtggag ggggttgtga tagtcgaaga
     2821 cgatgacgcc gtggaagccg tcgtacttgg cgacgttcga ggccgagatc gcgtggtcgc
     2881 cttccacgcg gccgaagacg tcggccggcg tgccttcctt tgggtggcaa aacggatctc
     2941 cctcggtcgc cgctatctcg cggtcgaggt ccaggctgac gcccgtgtcg agtggacggg
     3001 aggcccgcaa ctcgttgaag agcgtgcctt ccagggtgat gcggttcgtg accttcacga
     3061 tgcgctgggc ggtgacgtgg tccaccgagc cgaaggactt ctcgatccag gcgtgcatag
     3121 cctcaggcgg ggcgagacgt cccgtcgtcg tcgagacgtc gaagatgcgg tttgcgagga
     3181 tgcggtcttc cggggagagg tcggagatca ggtcgggaag gtccgttatc ttgcgttcgg
     3241 ataccatcgg ctatggagtc cgctctccgg cggacttcag gcctgcgtag aggccctcgc
     3301 ggtcgatcat acgctccacg tgtccctcgg ccctcaattt caggctcgct acctcgtggg
     3361 cgaagcggac ggcctcctgc cagtccttac cgtcgaggcg ggccatgagg agcgcagacc
     3421 agaaggcgtc gctggcgccc gtgacgtgcg agacctctac ctccggcaaa gggccgacgc
     3481 gctccagatt accgttgtcc gagacggtca ccacgccgcc ggagcgggtg aggatgacga
     3541 ccttcgcgcc gagatcgtgg aactcgctga tggcggtctc ctccagcgtc tcctcgtcca
     3601 tgttcacgtc gaagaggtgg cgggcgtcct cgagtgaggg cttgactatc gttacgtagg
     3661 gcatcacctc ggcgagaacc tcccaggcct cgaccttatc tggccagacg cgcggatcgt
     3721 agttcgggtc gagggtgacg atcttgccga ggcggctacc cagcctcatg gcccgcctga
     3781 cggcgaggcg ctgggggtcc ttcgagaggg cgaaagccga ggtgtggacg atgcgggccc
     3841 tggagatcat ctcctcgtct acctggcgaa cggcgaggag ggcgtcggcg cccctgttga
     3901 cctggaagtc agggacggtg acggtgcggg tgacgaaggc gttggtcgtc gaggcttcgt
     3961 ttgtgcggct tacggcttcg gtgttgacgc cgtgatgctg gagctggtcc tcgacgtact
     4021 cgccgaagta gtcggacccg agcttcgtga ttatggccga gcgtcccccg agtttggcca
     4081 cgtagacggc gacgttcgcc ggctggccgc cgaggtagcg ggagaactgg cccgccgtcc
     4141 tcaaagaatt ggccatctcg tgcgagatga agtcgaccat cgtctccccg acggcgagta
     4201 ggtcgagcct gtagtagagc tcgtccagcc cctccagcga gacaccgtct cccgaggccg
     4261 ggtctgtgaa gtaaggtggg atctcaagcc gcgccgggga acgggcctcg gagataaccc
     4321 cctggtcgtc gccagcgtgc agggtcgttg cgtcctcctc acgccctctc acagcgtcgc
     4381 tcctgcccga gatcgcttcg aggtatccct cagcggtctt ctcccaggtg tagcgggcga
     4441 gcacgcgacc gtagccggct ctggcgtaga gttcccagag ctcctggctg cctgtcaggg
     4501 tgtggagccc ggcgcccacc tcctccgggt cggtcgggtc gacgaggatg ccgtactcct
     4561 catcgccctc ccgcattgac tcgcttgggc cgccgaactt ggtgacgacc gcgggaaggc
     4621 ccgcggccat cgcctccagg ggagcgagcc cgaacggctc gtaaagcgcc gtgagcgcga
     4681 aaactgagcg ccgctctgct aggtagcggt atgtggcggc cagcgcccgc tggccgcgaa
     4741 cggcgaacat cgagacctcg ccccgcatcc gcgccctgtc tatgacctcc atcagggagt
     4801 cgaggacggc gcgttcttcg tccttggcag cgccgtagtc ttcgagcgga tcgtccaggc
     4861 ttccggtcag gatcaccaaa ttcgccgact cgcgcagggt agggctcccc gcgaacgcct
     4921 cgacgagcgc cagatggttc tttttgggat ccaacctgct cgaggcgacc acacaaggga
     4981 ggccggcgcg gtctggactc aggtcgcgct ccagggatgc cctgacgtac tctcggaccg
     5041 cttcttcctc ttggtagcgg gccgacttgt cgaagatgtg cagggcgacg ccaggcggaa
     5101 cggccgcgaa cctctcgtcg tccgtagggt cgacggcgcc cctgtaggcg ttatgggtgt
     5161 actggttgaa gcgctcctgg gaggtgctcg tgacgttgac gttggagcgg ttcatcgcca
     5221 gacgctctgc cacgatgcgc ctcgagaagt ggtagcgggc gtcgacctcg gcgatatctg
     5281 agcgtgggat ccccatcttg tccaacttct gcgcgcccaa agaatgggcc gtgaagctga
     5341 aagggatccc cgtgcgctcc tcgatcaggg ccgcgcagag ccccccgtcg ccgtagtggg
     5401 ccgtgacgac gtcgggcatc gatccctggt tctcgtagag ctcgagcaga cgcggcacga
     5461 actcggtgcc gagatagggc cagagctcct ctttggccag gaacgtgtca gggccgcaag
     5521 ggatgcggag gatgcgtacg ttcggggcgt ccggatacgc gtctgtgtcc gaggcgaact
     5581 cgggccactc ggggtctacg atccggcgcg tcacgatgtc cgccttgtga ccaaggttgc
     5641 ccatggccag cgccagttcc ttgacgtaga cgagctgccc gccgaagtcg gggtgggccg
     5701 tccagtagga gtcgtcggga tcgaaattcc cctgcgggtt tatgaaggcg acgtgcatcc
     5761 gctcatcctt tctgtccgtc ggactttgcg ccttgactcc ttcgtaggcc ggcgtaaagc
     5821 tcctcgcgct cgatctcgga gggcagaggg cctttggtcg tcagcttccg ctccacgacc
     5881 tcacgggcga agagcacgga tcgttcggcc gagttgccgt cgagtgtggc cgtgaggaag
     5941 cccgcccaga aggcgtcccc ggcacccgtg acgtcgacga cctcgacggg cctggcgggg
     6001 acgtgggtcg tgatcctgcc gttctcggag atgaggttgc cctcctcgcc catggtaaag
     6061 atgacggtct cggcgccgag gtcgtggaac atctcgatgt actgctcggg ttggtagtcg
     6121 gggccgaaga cccgcctggc gtcgacggcg gagggcttgg tcacgttgac gtgacgatag
     6181 gcatcgcgga tgaccttcct ggcctccttg tagtcgggcc agacccgcct gctgtagttc
     6241 gggtcgagcg agacgatctt gcccatctca cgcgctttgc ggaaggcttc tagcaccgcc
     6301 gaacggcaag gttcgagaga gagtgcgaag gtcgacgcgt ggactatcct ggcgcgcgag
     6361 atggcctctt cggagatctc ctgtggcctg agcagagagt cgccgcttcg gaactcttcg
     6421 aaatcagggg tcgctgcggt ggaggagatg aagatgacgg tggtgtgcgt gcggtggtcc
     6481 ataagcaggt agtccgtggt gacgccgtgg cgctggagct cgctcttgag gaattgcccg
     6541 aaagccccga tcccggtctt ggcgatcacc gccgaggtgc ccccgagttt ggagacgtag
     6601 accgcgatgt tggccggcga ccctcctaga tgccgttcga aggtcgacgc attactcaac
     6661 gtatcggtct tctccacgga gatgaaatcg acgaccatct ccccgatggt gaggacgtcg
     6721 aggggttctg tctctggtgc cataggcagc cagtctaatt aaccttcagg ccccacgtcc
     6781 actcctcact cgagaggaac ggacgggtag cgcgtggctc cgctcacggt gcgtggacgc
     6841 gaaagcggta tagactcaag gtaaagaacg atctctagtg caggagaggt tttggattat
     6901 tcgaacggcg gtatgggagg taagactgtc ctcatcacgg gcggcacctc cggtatcggg
     6961 aaggcggcgg cggtggcact ggcggcgatg ggcgcgaacg tcgtcgttgt ggggcgcaat
     7021 cgggagcgcg gtgaagcggc gatcgaggag attagcgccc ggagccacaa cgaatctgtg
     7081 gaactgctgc tggctgacct ctccgtgcag agcgaggtca ggggattcgc ccaagagttc
     7141 caggagcgcc acgacagaat cgatgtgctc gtcaacaacg ccgggctggt gcagagcaag
     7201 cggacggaga ccccggacgg catcgagacg acgctggcga taaaccatct ggcgccgttc
     7261 ttgctcacca acctgttgct agaccggctc aaaggtagcg cgccgagccg tgtgctcacc
     7321 gtctcctcag aggcgcagcg ttgggggact atggacttcg acgacctgca gtccaggcgc
     7381 aagtacaggg gcttccccgt ctacgggatg accaagctcg ccaacatcat gttcacctac
     7441 gagctcgcgg agcgcctgaa cggtacgggc gttagtgcga actgtctcca tcctggctcg
     7501 gtgggcacga acttcggcca gaacaacagg ggcccgatgg cgctcttttt caggaccttc
     7561 aagcctttca tgcgctcgcc agaacggggc gctgataccc tggtctggct cgcttcctcg
     7621 cccgaggtcg agggggttag cgggaagtac tactttgacc gcaaggagat cgaagccaag
     7681 aaggtggcct acgatcccgc tgcccgccgc aggctctggg agatcagcga ggaactgacg
     7741 ggcctgaagg tggccgccta gtgggccggg agaggggcat agaggtccag aacctcgtca
     7801 ggaaattcag gaagggaccg cgggccgtgg acgggataaa cctgcgtgtg gagccgggcg
     7861 aggtctacgg cttcctcggt ccgaacgggg ctgggaagtc gacgacggtt cttatgctga
     7921 ctacgctcct tccgcccacg agcggcacgg ccaccgtggc cggctacgac atcgtgcatg
     7981 agggaccgca ggtgcgggcc tttatcgggg cggcgctgca ggaggcggct ttggatcctt
     8041 tcctgacggg ccgcgagcac cttcgcctgc aggccgcgtt gcacggactc tcgcgcaagg
     8101 agcgcaaacg tcgcgggcag gagctgctgg agcgcgtcgg tctcgaggag gctgcggaca
     8161 ggaaagtacg cggctactcg ggcgggatga agcgccgcct cgacctcgcc ctcgcgctgg
     8221 tgcacggtcc gcagatcctc ttcctggatg agccgacgac gggcctggac ccgcagagcc
     8281 gcagcgcgct gtgggccgag gtcagcaggc tggcgcgcga tgagggcgtc accgtgttcc
     8341 tgacgaccca gtacctcgag gaggcggacg tgcttgccga ccgagtcggg atcatcgacc
     8401 gcggcaagat cgtcgccgag gacaccccgg aggccctgaa ggccgagatc ggacgcccca
     8461 gtgtcgaggc gacgcccgcc gagcgctcgg agcgcgacgc cgtcgcacgc gtgcttcggg
     8521 agtttggaga ggagatcccg gcccaaccag gcgccgtggc cgtgaggctc tccagcgggg
     8581 ccgccggcct cgccgacgtg gtgcgcgccc tcgatagggg ggaattacgc gtcgccaacc
     8641 tccgtctgga cgagcccagc ctcgacgacg tcttcctcgc caagacggga cgctcgctgg
     8701 aaggagccgg ggaggacgaa gagggtgaga tggacgaggt gcacgcgtga ggttctcgac
     8761 attcctcacc gaagttatgg cgatcgcacg ccgctccacg gttcgtaccc tgaggcaacc
     8821 cgcggtcatc gtaccttctc tactcttccc gatgttgttc ttctccatca acgcgaacgg
     8881 actcgacgcc gcctcgaaga tccccggttt ccctgccgac tcatatctcg atttcgcgtt
     8941 cgctttcccg ctcatacagg cctctctatt cggcgcgatc acggccggcg cggacctggc
     9001 ccgcgacatc gagagcggct tcttcgacag gctctcgctc accccgatgc gtcccgtggc
     9061 gttgctggga gggatgctga ccggggtggt cgcgctcggc tttctgcaag gcatcgtctt
     9121 cctcacgatc gggtcgctga tgggcgtcga cgtgagtagc gggtttttgg gcatggtcgt
     9181 gatcgtgctc cttaccgtgc tcgtcgccct cgggttcggt ggcatagggg cgatactcgc
     9241 gataaagacc ggttctgtgg aggcggtaga gagtgcgttc ccgctgttct tcgtggccat
     9301 ctttctgtcg tcgatcaacc tgccgcggga cctgatcgag caggactggt tccgatttat
     9361 cgcgaccatc aaccctatct cttacctggt cgaaggtatc cgcagcctgg tgatcaccgg
     9421 ctgggacccg caggcccttc tcctcggatt cggattcgcc ctggcgatcg tcgtggtcgc
     9481 catcggcggg gctgcggcgt cgctcaagac gagggtggcg tagggatggg cggcttcttc
     9541 gatgtggcgt ttgccgtggc ggggcgccag atatacaagt acttcacgaa ccccgctttc
     9601 ctcgcccccg cgctcttccc gcttttcttc ttcgtcgcct tcgccggcgg cctgacgagg
     9661 gtggccgacg tgcctggttt cgactacacg gccggctaca tcgcgttcca gtacgtgtgg
     9721 gccctgttgc aggccgtctc gatgggcggg gctttcacgg gattttcgat cgctggcgac
     9781 ttcgagaacg gtttcgccag gaggctcatg ctggccgcct cgaacaggta tgggatcatc
     9841 ctcggctaca ccatcgcctc gatcgtgcgt gccgccatga cggggattct cgtcaccgtg
     9901 atcgcgctgg tcacggggat gcccataagc ggcggctatg acttcttcgg cctcgtcgcc
     9961 ctcgcgctgc tcgccaatat agcggcgacc ctcttcggtg cgggggtggc tatgctgctc
    10021 cgcacccagc aggccggccc cgtgatacgc acgccgatct tcctcgtcct gtttctggcg
    10081 ccggtcttcg tgccgctgaa gttgctctcg ggttggctcg aggccgtcgc ccgtctcaac
    10141 ccgttcaccg ccatcctaga agccgcccgt ggctttctgg ccggcgaccc gacgggtgtg
    10201 gcttcggcct tcatcataag cgcggtactc atactggtac tcgccctctg ggccgtctac
    10261 ggtctccgtc gcgcggagag ggccggatag caaaggagcg ccgaatgact gcgagcgaag
    10321 gcaagaggac caccgagaag gatctctcag gtccggccag gttggcattg aagctgggga
    10381 gcgtcgcgca cgcgggggtg taccgcgcca cgggcggcaa ggtgttcggc cgtatgggca
    10441 agggtcccat cctgctcctc aacaccgtgg gtcggaagtc cggcaagaag cgtacgagcc
    10501 cgctcctcta cgtcatggac ggcgaggatt tcgtcgttat cgcctccaaa ggcggtgcgg
    10561 cgacgcaccc tgcctggtat ctgaacctga gggccaaccc ggaagcgacg gtcgagatcg
    10621 gggaccgtga ggtgcaagtc gaagccgaag tggcagaccc cgaggataag gcccgcctct
    10681 ggcagaagat ggtcgagatg tatccggcct acgacgacta ccagaagaag acggagcgag
    10741 agatcccgct cctcatactg cgtcctgtgg aagggagacc atcgtgaaga ccataagccg
    10801 cgaagacttg agagagaaga tcgaccgcgg cgacgatttc gtcctgctcg aagcactctc
    10861 cgaagcctcc tacgagcggg tccatctacc gggtgcgacc cggttccagg atatggactc
    10921 catcccagac cttctgccag acaagactac cgaggtggtg gcttactgct cgagcttcga
    10981 ctgacactcc tcgacgcggg tcgtccgtga actgacggct atgggttacg agaatgcctt
    11041 cgagtacgag ggcggcaaac aggactggat ggacgccggc tatccgaccg agagcggata
    11101 aatagatgta gcagtaggtt cgctttttgg tagactcctt tgcgtcgctc ccggagaggt
    11161 gtccgagttg gccgaaggag cgcgattgga aatcgcgtag gcggtgttaa gtcgtctcgt
    11221 gggttcgaat cccaccctct ccgcttttcc ttctgtttgc aggtaaaacg tggaagtcct
    11281 gcaaggtgaa aatgaggtcc agaattggct cgggacctta tctactgcca agcgtactgc
    11341 aacccgatag cttagagcgt cttcgagggc tctggtgagg tggttctgca tgtcgaagag
    11401 gacgtgagag taggtgtcga gggcgatgcc tatggtggtg tggccaagga gttcctctaa
    11461 cgatcctggg tgagctcctc tcctaaggtg cagtgtggcg gaggtcgtgg aagcggatgg
    11521 ggcgtagccc agcatatagg agcaacgcct tgaagctccg ttggtttatg ttgcgtcaag
    11581 gagcttgcca atctctgtag caaagatgaa ttctaatcgc agtgcagggt atgcgcgtta
    11641 gggatcaaat ccgctccgta ccggcttcca gcaagcccat gctcgttcta gatctcattc
    11701 atgatgagac tctaggtcag cggttcgagt ccactcgttg gctctccgta cggcatcact
    11761 atcgtgagcg cataatgctc actcagagag ctgctaaaaa gcagccgaac gtcacttcag
    11821 cgctgcacac aacattgtgg caaagggcac cgaatagagc aggtacttgc gcctctcctg
    11881 cttcagcgcc catagcgctg ctggcagata gtaggagagc aacagaagtg ctgccgcgcg
    11941 ttcggcccct gggccacccc tgtcatctgg gtcgggtacg tcggcgacgt tgcctgtgat
    12001 ctggaggcgc tggtacgcta gcgtaacgcc ggtcgtcaag gccagccagc cag
//
LOCUS       DATMVF010000172         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_661508, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000172 DATMVF010000000
VERSION     DATMVF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661508"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VN178_08605"
     CDS             complement(<1..576)
                     /locus_tag="VN178_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWS81071.1"
                     /translation="MNPATVLHATRIFDGERLLDSPHEVHVEEGRITAVLPSRGTGEN
                     VSTAFEEATLLPGLVDAHVHLSFDASPDVGALARADTSSTAVLRSAMNARRHLAAGVS
                     AVRDLGSPQGIVIDVARAVGTPLLPTAPTIVAAGQVLTITGGHGYFMGREVDGPEAVR
                     RGARAEIKRGARALKVIATGGVITEQGTPGAT"
     gene            complement(791..1822)
                     /locus_tag="VN178_08610"
     CDS             complement(791..1822)
                     /locus_tag="VN178_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWS81072.1"
                     /translation="MTSDMYREIHEQPEVLTTILEEEWETVAHAARTLRSRGFRFAML
                     AARGTSDNAALYAKYLFEIRLGVPVSLAAPSTFTLYESEMNLEDVLVIGISQSGESKD
                     VLEVLRRSRQLGAITLSVTNDESSSLASVADVHLWLHAGREKSVAATKTYTAELLLLY
                     LLIAALEERSKSEPEVEQLPDMAKQVLDVAWEGLDRYRYAHYMTVASRGYNFATAKEA
                     ALKLMETTYTVTEAFSEADLRHGPMAMIGRDFPVVLIVPPGRTYQSMDSLAKGLASRG
                     AELAVIGEDPDLVGEAAAGFLVPVSCPEELSPILYALPAQLLAHSVAELRGLDPDSPR
                     GLSKVTETW"
     gene            complement(1914..>2429)
                     /locus_tag="VN178_08615"
     CDS             complement(1914..>2429)
                     /locus_tag="VN178_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWS81073.1"
                     /translation="YNAMRPLAHREPGTVGALLTDDRLRVGIIPDGVHVHEAALRLAY
                     RAKGSERLILVTDAMEAAGMAPGDYELGGRRVHLEAGAVRLPDGTLAGSALTMDEAVR
                     NATEFLGVSLTEAVRMASEVPASALNLEGKGRIAAGCDADFVLLDAQGIVLETIVGGK
                     TIYDREENEHQ"
BASE COUNT          447 a          779 c          737 g          466 t
ORIGIN      
        1 agtcgctccc ggtgtacctt gctcggttat gacaccgccg gtagcgataa ccttgagtgc
       61 ccgggcgccg cgtttgatct ccgcgcgcgc cccgcgacgg acggcttcgg ggccatcgac
      121 ctccctgccc atgaagtaac cgtgcccgcc cgtgattgtc agtacctgtc cggccgccac
      181 tatagtaggg gccgtcggaa gaagtggagt gcccacggcg cgggcaacgt cgatgacgat
      241 cccttgtggc gaaccaaggt cgcgaactgc gctaaccccc gccgcaaggt gtctgcgggc
      301 gttcatcgcc gagcgcaaca cagcggtcga agacgtgtcg gctcgcgcca gagctccaac
      361 gtcgggcgac gcgtcgaagc tgaggtgcac gtgcgcgtcg acgagtcccg gcaggagcgt
      421 cgcctcttcg aaagccgtcg agacgttttc gccagtgccc cgggacggta gcaccgcagt
      481 tatgcggcct tcctcgacat gcacttcgtg ggggctatcg aggagacgct cgccatcgaa
      541 gatgcgggta gcgtgcaata ccgtcgcggg gttcatgcgc agggttccgg ggtatgagac
      601 ccttccgccc gggcgagtaa atcgtgaaac acgagcgggt cggcgtccgc cagctgagcg
      661 aggcgcccca cgcactcggc gccagcctcg gcggcgtcgc cccagacgcc ctggatgtcc
      721 cccggggttt cgcggaggac ggttcgcccg tcgaacaagg ccaagacgat atgccctcgg
      781 ctctccaatg tcaccaggtc tcggtcacct tgctcaagcc gcgtggagaa tccgggtcga
      841 ggcccctcag ttcggctacg ctatgcgcta gaagctgggc gggcaaagcg tagagtatgg
      901 gtgagagctc ctccggacac gagactggca ctagaaagcc cgccgctgct tcaccgacca
      961 ggtcgggatc ctcaccgatc accgcgagtt ccgcgccccg ggaagccagg cctttggcca
     1021 atgaatccat gctttggtag gtccttcccg gtggcacgat cagcacgacc gggaaatcgc
     1081 ggcctatcat cgccataggt ccgtggcgaa ggtcggcctc cgagaaagct tcggtaacgg
     1141 tgtaagtcgt ctccatcaac ttgagagcag cttccttggc cgtcgcgaag ttgtaccccc
     1201 tggaagccac ggtcatgtag tgggcgtatc ggtagcgatc tagaccctcc caagcgacgt
     1261 ccagcacctg tttcgccata tcgggaagct gctcaacctc cggctctgac ttcgaccttt
     1321 cttccagcgc tgcaatcaag aggtaaagca agagcaactc ggcggtgtag gtcttggtgg
     1381 ccgcgacgct cttctcccta cccgcatgga gccagaggtg aacgtccgcc accgaggcga
     1441 gagacgaact ctcgtcgttg gttacggaga gggtgatcgc ccccagctga cgtgaccttc
     1501 gaagaacctc caggacgtcc ttactctccc cggactggct gatgcctatc acgagcacgt
     1561 cctcaaggtt catctcgctc tcgtacagtg tgaaggtcga cggagccgcg agggagacgg
     1621 gcacacccaa gcgtatctcg aagaggtact tagcgtagag ggcggcgttg tccgaggtac
     1681 ctcgagcggc gagcatcgcg aagcggaagc ctctgcttcg cagggtacgg gccgcgtggg
     1741 caaccgtctc ccattcctcc tcgaggatag ttgtcagaac ctccggttgc tcgtggatct
     1801 ctctatacat gtcgctcgtc acaacggcat ctcctcgttt cgaccaccag aattgttgcg
     1861 ggctggcgcc ggtcgtattc tccttagcac agactctgat gccacgttag gattcattga
     1921 tgctcgttct cctcacggtc gtagattgtc tttccgccca cgatggtctc gagcacaata
     1981 ccctgcgcgt ccaatagcac aaagtcggca tcgcaaccgg cagcgatcct acccttgccc
     2041 tcgaggttca aggccgaagc gggaacctcg gaggccatgc gcacggcttc agtgagggag
     2101 acaccgagaa attcagtggc gtttcttacg gcttcgtcca tggtgagcgc gctcccagcg
     2161 agtgtcccgt cgggaagccg caccgctcca gcttctagat gaaccctccg tccgcccaat
     2221 tcgtagtcgc ccggggccat acccgctgct tccatggcat ccgttactag gatcagacgt
     2281 tctgagccct tagcccgata agcgagacga agtgccgcct cgtggacgtg cacaccgtcc
     2341 gggatgatgc ctaccctcag gcggtcgtcg gtaagcaagg cgcctacggt gccgggttcc
     2401 ctgtgagcca gcggtctcat cgcattgta
//
LOCUS       DATMVF010000173         9009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_65278, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000173 DATMVF010000000
VERSION     DATMVF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65278"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..296)
                     /locus_tag="VN178_08620"
     CDS             complement(60..296)
                     /locus_tag="VN178_08620"
                     /inference="COORDINATES: protein motif:HMM:NF042609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HWS81074.1"
                     /translation="MGSRRYTIKQLPPELRPRERLLSDGPEALSSAELLAILLGTGSK
                     ESTAVELAGQVISESGDLFGLHGVSIQVPNYKTR"
     gene            complement(826..1389)
                     /locus_tag="VN178_08625"
     CDS             complement(826..1389)
                     /locus_tag="VN178_08625"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWS81075.1"
                     /translation="MLASESARRVDLLRTAGYEFEAHKSGFPEVVFEDPIKTVRANAE
                     GKALAVATDPDTVVLAADTIVYLPDLPTHDRILGQARNEQEVQRFIDLLQGRAHEVFT
                     GIAVTRGEDLVSRHAMTRVRMRSLGTAEVESYARYGEGIGKAGGYAVQGRAASFVEWI
                     GGDYTNVVGLPLALTRRMLTNAGIPHP"
     gene            complement(1404..1805)
                     /gene="ndk"
                     /locus_tag="VN178_08630"
     CDS             complement(1404..1805)
                     /gene="ndk"
                     /locus_tag="VN178_08630"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWS81076.1"
                     /translation="MEQTLVLIKGDGVRRRLVGEIVGRIEARGLDIADMKLMDVGIDL
                     AEEHYAEHREKPFFDELVEFITATPVVAMRISGEGAIKAMRALMGATNPADAAPGTIR
                     GDLALSMPDNLVHGSDSPQSAERELGLFFGP"
     gene            complement(1817..2311)
                     /locus_tag="VN178_08635"
     CDS             complement(1817..2311)
                     /locus_tag="VN178_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWS81077.1"
                     /translation="MRFSLAFQQDPDPFQIISDFLESPILRISGQLVLLLFIVLWVSL
                     VYWTYADASRRGAMSVLWGFIAFIFPYVGTLVYLIIRPPEYLEESRERELELAVLERE
                     LTNSVLLCPNCRNLVEKDYLICPSCTWELKKSCVNCEKPLNMEWGMCPYCGTDQRSGK
                     TILR"
     gene            complement(2399..3610)
                     /locus_tag="VN178_08640"
     CDS             complement(2399..3610)
                     /locus_tag="VN178_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HWS81078.1"
                     /translation="MTTFAQVCDELDRRRHVTLGLERIEALLKAMGNPESHVPGLVQV
                     VGTNGKGTTAVALAAALEEMGYPSGAYLSPHVLSYTERVVLRGAQVSEGVFSTAMGET
                     IELADRSKIPASQFELLTAGALGMFAAAGLSWAVLEAGLGARHDATSAARLDSVVLTN
                     VSIDHTEYLGETVEEIAEEKLASLKPGATLFLGTDESRIVDLAHSACRRVGGKLVLPG
                     QPGEDAPFPGLAPYEADDVRLGISVAESLLERSLPAGVEQKVARAVREALPGRFEIHQ
                     VEGVPVVVDGGHNTAGLSAALAGMRAVYGKRPLGVVFGVLREKDAGSMLAGLEGEADY
                     IVLTRPQSERAADPADLEYPHLESRARVVEHPVEALGVAVEAVRDAAGVVLVTGSLST
                     AAPVLRWLREA"
     gene            complement(3629..6175)
                     /locus_tag="VN178_08645"
     CDS             complement(3629..6175)
                     /locus_tag="VN178_08645"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWS81079.1"
                     /translation="MSQQIPKTYDHRAVEGRWYEEWERRGAFEADPNPDKEPYCIVLP
                     PPNVTGALHMGHALNGSIQDTLIRRARMKGYEALWLPGVDHASIGLHNVVERKIMREE
                     GKTRWDIGREEFVERCRVFADEARSQILGQLKRLGASVDWRKLAYTMDEQYVDAVLTA
                     FVDLFDSGLIYRENRITNWCPHDRSAISDLEVNYEEVEGKLYGLRYPFVDDKGPGPDG
                     RGYAQVFSTRPETMLGDVALVVNPDDERYTELVGRTVKVPFVEREIEVFADDYVDASF
                     GTGVLKVTPAHDPNDFEIGKRLRLEPVNVLEPDGTINAYGGPFEGMSREDARKAVVAR
                     LSEEGLLGEVREYPHRVGHCDRCGAVIEPWLSEQWWISMKELAEPAIEALKNKEITVY
                     PDSWRRETIRWLENIHDWNVSRQLWWGHRIPVWYGPEGEVVASKESPGEGFEQDPDIL
                     DTWFSSGLWPLATLGWPEETEELAYFYPTSLLSTAREIMYLWVARMIMMGLRFRNGVP
                     FGKVNVHSIVLAEDGTKMSKSKGNTINPLDLFDRYGTDAVRFGLLYQSSTQDFAYSYE
                     RAEMGRAFVTKLWNATRFVLAYPEGEDAGELSVSDRWILSEFDRTIGEYDAFLEECEF
                     SEAMRRIYSFAWNQFADWYIEIAKAAPSSATPRILREVFHGILKLLHPVMPFATEEMA
                     SKMGEHALLATQSFPEPDPVRRDEEADRLLDRTKRAVSAVRSFRAESKVDGELEGLVP
                     QGIEEGVFAALAGVRPVEAGDGASRASLPAGDVVVEILLSDEMRRGEIERLRREISRA
                     EQEVARSQSKLANEKFVDNAPEKIVAGEREKLDVNSRMLETLSRRLDEYL"
     gene            complement(6194..7453)
                     /gene="clpX"
                     /locus_tag="VN178_08650"
     CDS             complement(6194..7453)
                     /gene="clpX"
                     /locus_tag="VN178_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWS81080.1"
                     /translation="MRDPSDQLQCSFCGKSQRQVRKLIAGPGVYICDECIELCNEIID
                     EEFSGPEVLKDDDLPKPREINRILNEYVIGQEDAKKVLAVAVYNHYRRIQLGAEATDG
                     TELQKSNILLVGPTGSGKTLLAQTLARILNVPFAIADATALTEAGYVGEDVENILLKL
                     IQAADFDVKKAETGIIYIDEIDKVARKADNPSITRDVSGEGVQQALLKILEGTVASVP
                     PQGGRKHPHQDFLQIDTKNVLFICGGAFGGLEEIIRQRIGKKSIGFGSDIDSRLKQEE
                     DSILQFALPEDLLKYGLIPEFVGRLPVISTLRSLEEADLVRILTEPKNALVRQYRQFF
                     RYDKVDLTFTDDALGAIAELALERGTGARGLRSILESALLPTMYELPSRSDVNKCVVD
                     VDTVKDGVQPSLVTGSQKYSYDEEESA"
     gene            complement(7501..8094)
                     /locus_tag="VN178_08655"
     CDS             complement(7501..8094)
                     /locus_tag="VN178_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWS81081.1"
                     /translation="MSIDNVIPYVIEQSPRGERAMDIYSRLLKDRIIFLGTPVDDQVA
                     NAIMAQLLHLESEDPEQDINLYINSPGGSVTAGLAIYDTMQFVKPDISTTALGMAASM
                     GAFLLAAGAKGKRNALPNTRILLHQPSVGGLAGQASDVEIHAKELIMTKRRLNEILAE
                     NTGQDYERIERDTDRDYIMGPEEGIEYGVIDNIVRNH"
     gene            complement(8091..>9009)
                     /gene="tig"
                     /locus_tag="VN178_08660"
     CDS             complement(8091..>9009)
                     /gene="tig"
                     /locus_tag="VN178_08660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWS81082.1"
                     /translation="VQEGDFVILDFKGEKLAGGPLEGAEAEDYLLEIGGGELLPDFEE
                     NIVGMNANERKRFGVTFPMDYDEETLRGQSVLFNVHAKEIKERDLPPLDDEFVKEASE
                     FENLEELRVAVREELEATAEQRAEGEFRARVLEVVAEGGEVEVPDVMAHEKAHEMVES
                     FERSLRSQGMDPEQYYELAGTDHAAFEERVMPDAEDTVRKELVLDAVATAEGIVADED
                     EIMHEIGHLAEGSETSTEEIAKTLRDNGTYALLEEEISRQKALDFLVENAVPVPISEE
                     ETEEETAGEEESVEARAGATESEEREEQQ"
BASE COUNT         1488 a         2883 c         2798 g         1840 t
ORIGIN      
        1 tcccccactc gaagcgcacc tgttgggcag ccttgcccag cactgcccga aagcgtagct
       61 cagcgagttt tgtagtttgg gacttgtatg gaaacaccgt gcaatccgaa gaggtcgcca
      121 ctctctgaga tgacctgccc ggcaagctcg accgccgtac tctctttgct gccggtgccg
      181 aggagaatgg cgagcaactc ggcgctggaa agcgcctcgg ggccgtccga taacagccgc
      241 tcccgcggcc tgagctcggg agggagctgc ttgatcgtgt atctgcgaga tcccacggcg
      301 gtattttatc ttatacaggt gccggcaggt tgccacctgc gcactcaggc gtgacttctc
      361 cggccccttg ccggtcgagt gaaccttggt ggcaaagcgt ggagacccgc cctgctcgac
      421 ttcggacgac ttcgcgctcc ttgatctgct tggaaggatc cgaacgaagc cggccgggct
      481 cgagttcgtt ctcgaaaatg ggccgaattg tgtattgtgc cttgctcgat ctgtgcgatc
      541 atagaacttt gtaagtgcat gacccgacgt gacttttagg agggggacgt tgggctgtgc
      601 actatgtcgt tactctcgtg cgagcaggcc cgctatgtat cctgctccgt aagtgaaggc
      661 ggcacagcga gaggaagggc gggggcgcaa agtttctcag cacttcgcgc catgcttcca
      721 tccgctggat cgctttcgat gggaggagcg gagcatctgg ggtggtggat tcccgcttgc
      781 gcgggaatga cagcgatcct gtataaagcc ttgcgcgctg acccttcagg gatgtgggat
      841 tcctgcgttg gtcagcatcc tgcgtgtcaa ggcgagcggc aggccgacga cgttggtgta
      901 gtcgccgccg atccactcaa cgaaagatgc tgcccgcccc tgcacagcgt atcctcctgc
      961 cttacctata ccctctccgt agcgggcgta ggactcgact tcggcggtgc cgagggatcg
     1021 catgcgaacc ctggtcatgg cgtgccgtga gacgagatcc tccccccttg ttacggcgat
     1081 tcccgtgaag acttcgtggg cgcgtccttg cagcaggtcg atgaatcgct ggacttcttg
     1141 ttcgtttctg gcctgtccga ggatgcggtc gtgcgttgga aggtcgggca ggtagacgat
     1201 cgtatcggcc gcgagcacga cggtgtcggg gtctgtcgcg acggcgagcg ctttgccctc
     1261 cgcgttggcc ctgacggtct ttatggggtc ttcgaagacg acctcgggga agccgctctt
     1321 atgagcctcg aactcgtagc ctgccgtgcg gagcagatca acccgcctgg ccgactccga
     1381 ggcgagcacg aggcgcgatg ctttcaaggg ccgaagaaga ggcctagctc ccgctctgcg
     1441 ctctgtggtg agtccgagcc gtgcacgagg ttatccggca tgcttagggc caggtcgccc
     1501 cggatcgtac cgggcgccgc gtcggcgggg ttcgtggccc ccataagggc cctcatggcc
     1561 tttatcgcgc cttcgccact tatcctcatg gcgacgaccg gtgtcgcggt gatgaactcg
     1621 accagttcat cgaagaaagg cttctcgcgg tgctcggcgt agtgttcttc ggcaagatcg
     1681 atgcccacgt ccatcagctt catgtcggcg atgtcgaggc ctctggcttc tatgcggccg
     1741 acgatctcac cgacgagcct gcggcgcacg ccgtcgccct tgatcagtac cagcgtctgt
     1801 tccaattctc tactccttat ctgaggattg tcttgccgct gcgctggtcg gtgccgcagt
     1861 aagggcacat cccccactcc atgttcagtg gtttttcgca gtttacgcaa gacttcttga
     1921 gttcccaggt gcacgaaggg cagatcaggt agtccttttc gaccaggttg cggcagttgg
     1981 ggcacagaag gacgctgttc gtcagttcgc gctccagcac cgccagctcc agctcgcgtt
     2041 cgcgggactc ttcgaggtat tcgggcggac gtatgatgag gtagaccagg gtgccgacgt
     2101 agggaaagat gaaggcgata aagccccata gcacgctcat ggccccgcgc ctggaggcgt
     2161 cggcgtaggt ccagtacacg agagagaccc acagcacgat gaaaagaagg agcacgagct
     2221 ggcccgagat cctcaggatc gggctctcca gaaagtcgga gatgatctgg aatgggtcgg
     2281 ggtcctgctg gaatgcgagg gagaatctca tccgataaac cttaccatca ctaggtagcc
     2341 ggccacaaaa gataccacgg cgaccaccaa tagcaggccg ataagctgcc aggcgccctt
     2401 atgcttcacg caaccaccgc agtaccggag ccgccgtgga gagggaccct gtgacgagga
     2461 ccaccccggc ggcgtctctt acagcctcca cggccacgcc gagggcctct acaggatgct
     2521 ctactaccct ggccctgctc tcgaggtgag ggtactcgag gtccgcgggg tcggcggccc
     2581 tctcgctctg aggacgcgtc agcacgatat agtcggcttc accttccaac ccggcaagca
     2641 tactcccggc gtccttctct cgcaagacac cgaagacgac gcccaaggga cgcttgccgt
     2701 acacggcgcg catcccggcg agcgccgccg agagcccggc cgtgttgtga ccgccgtcga
     2761 ccaccacggg aacaccctcg acttggtgta tctcgaaccg tccgggaagc gcctctctga
     2821 cggcccgcgc gaccttctgc tcgacccctg cggggaggct cctctcgagc aacgactcgg
     2881 ccaccgagat ccccagtcgc acgtcgtccg cctcgtacgg cgcgaggccc ggaaacgggg
     2941 catcttcgcc aggctggccg ggcagaacca acttcccccc gacccggcgg caggcgctgt
     3001 gggcgaggtc gacgatccgg ctttcgtccg taccgagaaa cagggtcgca ccgggcttca
     3061 ggctggcaag cttctcttcg gctatctcct cgaccgtctc acccaggtac tcggtgtggt
     3121 cgatggagac attggtgagc accacggagt cgagcctggc ggcagaggtg gcgtcgtgac
     3181 gggcgccgag gccggcctcg aggacggccc aagacagccc cgccgccgcg aacatcccca
     3241 gggcgccggc ggtaaggagc tcgaactgcg aagcggggat tttgcttctg tccgcgagct
     3301 cgatggtctc tcccatggcg gttgagaaga ccccttccga gacctgggcg ccgcggagta
     3361 cgactctttc ggtgtaggag aggacgtgcg gagagaggta ggcgcccgac ggatacccca
     3421 tctcctccaa cgccgcagcg agggcgacgg ccgtcgtgcc cttgccgttc gtgccgacga
     3481 cctgcaccag gccgggcaca tggctttcag gattacccat agctttgagg agggcttcta
     3541 tccgctccaa accgagggtg acgtgacggc gccggtccaa ctcgtcacag acttgggcga
     3601 aagtagtcat ggtagggatg gccggctcct acaggtactc gtcgagacgg cgagagagcg
     3661 tctcgagcat cctggagttg acgtccagct tctcgcgctc gccggcgacg atcttctccg
     3721 gcgcgttgtc tacgaacttc tcgttggcga gcttgctctg tgagcgcgcc acttcctgct
     3781 cggcacggga gatctcccgc ctcaggcgct cgatctcgcc gcggcgcatc tcgtcggaga
     3841 ggaggatctc cacgaccaca tcccccgccg gcaacgacgc ccggctcgca ccgtctcctg
     3901 cctccacagg cctcaccccg gccagcgcgg cgaagacgcc ctcctcgatg ccctgcggca
     3961 cgagtccttc gagttcgccg tcgaccttcg actcggccct gaacgagcgc accgccgaga
     4021 ctgccctctt ggttcggtcg agcaggcggt ccgcctcctc gtctcggcgc acggggtcgg
     4081 gttcggggaa gctctgagtc gcgagaagtg cgtgttctcc cattttgctc gccatctctt
     4141 ccgtcgcgaa gggcatgacg gggtgcagga gcttgaggat accgtggaag acctcgcgca
     4201 ggatgcgcgg tgtcgcgctc gagggggctg ccttcgctat ctcgatgtac cagtcggcga
     4261 actggttcca ggcgaaagag tagatccggc gcatcgcctc cgagaactcg cactcctcca
     4321 gaaaggcgtc gtactccccg atagtgcggt cgaactcgga gaggatccaa cggtcagaaa
     4381 ccgagagctc tccggcatct tcgccctccg ggtaggcaag tacgaaacgc gtggcgttcc
     4441 acagcttggt gacgaaggcc cgacccatct cggcacgctc gtaggaatag gcgaagtcct
     4501 gagtcgagga ctggtagagc agtccgaagc gcacggcgtc cgtgccgtag cggtcgaaga
     4561 ggtcgagcgg gtttatggtg ttgcccttcg acttactcat cttcgtgcca tcctcggcca
     4621 ggacgatgga gtggacgttg accttcccga aaggcacccc gttcctgaag cgcaggccca
     4681 tcatgatcat acgcgccacc cagaggtaca tgatctcgcg cgccgtcgag aggagactcg
     4741 tcgggtagaa gtacgccaac tcttctgtct cctcgggcca accgagcgtc gctaaaggcc
     4801 aaagaccgct gctgaaccac gtatccagga tgtccgggtc ctgctcgaag ccctcgcccg
     4861 gcgactcttt ggaggccacg acttcccctt cagggccata ccagacgggg atgcggtgcc
     4921 cccaccagag ctgtcgtgag acgttccagt cgtggatgtt ttcgagccac cggatcgtct
     4981 cgcggcgcca ggaatcgggg tagacggtga tctccttgtt cttcaaagcc tctatggcgg
     5041 gctctgcgag ctccttcatc gagatccacc actgctcgga gagccacggc tctatcaccg
     5101 ccccgcacct gtcgcagtgc ccgaccctgt gcggatactc ccgtacctcg ccaagcagac
     5161 cctcctcgct caggcgagcg acgacggctt tcctcgcatc ctcgcggctc atcccctcga
     5221 acgggccgcc gtacgcgttg atcgtgccgt cgggttcgag tacgttgacg ggctcgaggc
     5281 gtaaccgctt gccgatctcg aagtcgttcg ggtcgtgggc cggcgttacc ttgaggactc
     5341 cggtaccgaa ggaggcgtct acgtagtcgt cggcgaagac ctctatctcg cgctcgacga
     5401 acggtacctt caccgtgcgt ccgacgagct ccgtgtagcg ttcgtcgtcc gggttgacga
     5461 cgagggcgac gtcgccgagc atcgtctccg gccgcgtgga gaagacctgg gcgtagcctc
     5521 tcccgtcggg accggggccc ttatcgtcaa cgaaggggta tcgcaggccg tagagcttgc
     5581 cctccacctc ctcgtagttg acctccaggt cgcttatcgc ggagcgatcg tgcggacacc
     5641 agttcgtaat acggttctcg cggtatatga gcccggaatc gaacaggtcg acgaacgccg
     5701 tgagaacggc gtctacgtat tgctcgtcca tggtgtaggc aagttttcgc cagtcgaccg
     5761 acgcccccag gcgctttagt tgcccgagta tctgtgagcg cgcctcatcg gcgaaaaccc
     5821 ggcaacgctc gacgaactcc tcacgtccga tgtcccagcg ggtcttgccc tcctcgcgca
     5881 tgatcttgcg ctctacgacg ttgtggaggc cgatggaggc gtggtccacg ccgggtagcc
     5941 acagggcttc gtagcccttc atccgcgcgc gccggatcag ggtgtcctgg atggagccgt
     6001 tgagggcatg acccatgtgc agggcgcccg tcacgttggg cggcggcaag acgatgcaat
     6061 agggctcctt gtcggggttc gggtctgcct cgaacgcccc gcggcgctcc cattcttcgt
     6121 accagcggcc ctcgacggcg cggtggtcgt aggttttcgg tatctgctgg ctcaaaggga
     6181 aactgggagc gacctaagcg gactcttctt cgtcgtagga gtacttctgg gaaccggtta
     6241 ccagtgacgg ctgaacaccg tcctttacag tatctacgtc gacgacgcac ttgttcacat
     6301 ccgagcggct cggaagctcg tacatcgtgg gcagaagggc cgactcgagg atgctcctga
     6361 gaccgcgggc ccccgtcccc cgctcgagcg cgagctcggc tatggcgccg agggcatcgt
     6421 ccgtgaaggt caggtccacc ttgtcgtagc ggaagaactg cctgtactgc ctcaccaggg
     6481 cgttcttcgg ctcggtgagg atgcgcacga ggtcggcctc ctccaggctg cgcagggtgg
     6541 agatcaccgg cagacgtccc acgaactcgg gtattagccc gtacttcagg aggtcctcgg
     6601 ggagcgcgaa ctgcaagatg gagtcttctt cctgcttgag gcgggagtct atgtcgctgc
     6661 cgaagcctat gctcttcttg ccgatgcgct ggcggatgat ctcctcgagt cccccgaacg
     6721 cgccaccgca gatgaacagg acgttcttgg tgtctatctg gaggaagtcc tggtgcgggt
     6781 gcttgcgccc tccctgcggt ggaacgctcg cgaccgtgcc ttcgaggatc ttgaggaggg
     6841 cttgctgcac gccttcgccg gagacgtcgc gcgtgatcga agggttgtcc gccttgcggg
     6901 ctaccttatc gatctcatcg atgtagatga tgcccgtctc ggccttcttc acgtcgaagt
     6961 cggcggcctg tatgagcttg aggaggatgt tctcaacgtc ctcgcctacg tagcctgcct
     7021 cggtcagggc cgttgcgtcg gctatggcga aggggacgtt gagaatcctg gccagcgtct
     7081 gggcgaggag cgtcttgccc gagccggtcg gtcccacgag caggatgttg gacttctgca
     7141 gctcggtgcc gtccgtggcc tcggcgccca actggatgcg cctgtagtgg ttgtagacgg
     7201 cgacggccag gaccttcttg gcgtcctcct ggccgatcac gtactcgttg aggatgcggt
     7261 ttatctccct gggcttgggg aggtcgtcgt ccttgaggac ctcgggcccc gagaactctt
     7321 cgtcgatgat ctcgttgcac agctcgatgc actcgtcgca gatgtagacg ccggggcccg
     7381 cgatgagctt tctgacctgg cgctggctct ttccgcagaa ggagcactgc aactgatccg
     7441 acggatccct catgcctcta cgtcctcccc gacatctcta cccgatggcc ccaggaacct
     7501 ctagtggttc ctgacgatgt tgtcgataac gccgtactcg atcccctctt cgggacccat
     7561 tatgtagtcg cggtcggtgt cgcgctctat cctctcgtag tcctggccgg tgttctcggc
     7621 gaggatctcg ttcaggcggc gcttggtcat gatgagctct ttggcgtgaa tctccacgtc
     7681 ggaggcctgt ccggccagcc cgccgacgct cggctggtgc aggagtatgc gcgtgttcgg
     7741 cagggcgttc ctcttgccct tggccccggc ggcgagcagg aaggccccca tcgaagcggc
     7801 catgccgagg gcggtcgtgg agatgtccgg cttgacgaac tgcatggtgt cgtagatcgc
     7861 aagccccgcc gttacgctgc cgcccgggga gttgatgtag aggttgatgt cctgctcggg
     7921 gtcctcgctc tccaggtgca agagctgagc cattatggcg ttggccacct ggtcatccac
     7981 gggggtcccc aggaagatga tgcggtcctt caggagccgc gagtagatgt ccatggcccg
     8041 ctccccgcgc gggctctgct ctataacgta ggggatgacg ttgtctatgc tcattgctgt
     8101 tcctctcgtt cttcgctctc cgtcgcaccg gccctggcct ctaccgattc ttcttcgcca
     8161 gccgtttctt cctcagtctc ctcctcagag atagggacgg gcaccgcgtt ctcgaccagg
     8221 aaatccagcg ccttctggcg tgagatctcc tcttcgagca acgcgtaagt cccgttgtcg
     8281 cggagggttt tggcgatctc ctcggtgctt gtttcggagc cttcggcgag gtggccgatc
     8341 tcgtgcatta tctcgtcctc gtccgcgact attccttccg ccgtggcgac cgcgtcgagc
     8401 acgagctctt tccttacggt gtcctcggcg tccggcatga cacgctcctc gaaggcggcg
     8461 tggtccgtgc ccgcgagctc gtagtactgt tcggggtcca taccctgtga cctcagactc
     8521 cgctcgaagc tctcgaccat ctcgtgagcc ttctcgtgcg ccatgacatc ggggacctct
     8581 acctcaccgc cttcggcgac gacctcgagc acgcgcgcgc gaaactcgcc ttccgcccgc
     8641 tgctccgcgg tggcctccag ctcctccctt acggcgacgc gcagctcttc caggttctcg
     8701 aactcgctcg cctccttgac gaactcgtcg tcgagaggcg gcaggtcgcg ctctttgatc
     8761 tccttggcgt gcacgttgaa gagcacagac tggcccctga gcgtctcctc gtcgtaatcc
     8821 atggggaagg tgacgccgaa ccgcttgcgc tcgttcgcgt tcataccgac gatgttctcc
     8881 tcgaagtccg gcagaagctc gccgcccccg atctcgagca aataatcctc ggcctcagca
     8941 ccctccagcg gccctccggc cagcttctcg cctttgaagt ccaggatcac gaagtctcct
     9001 tcctggacc
//
LOCUS       DATMVF010000174         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_246782, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000174 DATMVF010000000
VERSION     DATMVF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246782"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VN178_08665"
     CDS             complement(<1..620)
                     /locus_tag="VN178_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWS81083.1"
                     /translation="MSEDLFEPLFVPDEIREALGGRAWLQAMLDAEAALALAEARAGL
                     IPDEAAVTIARYCDADRFDPEEIGRKGRAAGNPVPPLVRALTAAVSGEASGDAGSYVH
                     KGATSQDIVDTAAMLVARRAVDLILVEVDGIAAACARLADEHRATIMAGRTLLQQALP
                     TTFGLKAAGWLVVVLEARRQLFSVRESGLAAQLGGAAGTLASLGDSG"
     gene            complement(613..1194)
                     /gene="pcaG"
                     /locus_tag="VN178_08670"
     CDS             complement(613..1194)
                     /gene="pcaG"
                     /locus_tag="VN178_08670"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HWS81084.1"
                     /translation="MTPEPRLEPTPSQTVGPFFHFALTEEDRSRLVALDHPRAVRIEG
                     TVHDGAGEPVTDAMVEIWQANSNGRYAHPEDHRDELPLEEGFTGFGRCQTDTEGRFEF
                     VTVKPGAVPGPDGLPQAPHIDVAIFARGLLRQVVTRIYFPDEEEANAADPVLTSIEDH
                     DLRSTLTARGSQEGHLHFDINLQGEHQTVFFDV"
     gene            complement(1191..1919)
                     /gene="pcaH"
                     /locus_tag="VN178_08675"
     CDS             complement(1191..1919)
                     /gene="pcaH"
                     /locus_tag="VN178_08675"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HWS81085.1"
                     /translation="MAQQSRSGVAGYRRDDAEVHPPYLYPDYKGTRLRAPEKPLIILP
                     HTLTEMTGPVYGHEPVGSLDHDLTRQHKGEPLGERIIVHGRVLDGDGRPVRNSLVEIW
                     QANATGRYIHQGDRHPAPLDPNFSGAGRCLTDDEGRYRFVTVKPGAYPWGNHYNAWRP
                     AHIHFSLFGPAFATRLVTQMYFPGDPLFDQDPIFRSVRDPKARERLISSFDLEATEPE
                     WALAYRFDIVLRGREATPTERNHK"
     gene            complement(1938..>2206)
                     /locus_tag="VN178_08680"
     CDS             complement(1938..>2206)
                     /locus_tag="VN178_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564561.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HWS81086.1"
                     /translation="DDIGLIELNEAFAAQSLAVLYEWGMDPDNERLNLNGGAISLGHP
                     IGCSGARILTTLLHEMRRREDVELGLATMCIGVGQGIAMVVEKV"
BASE COUNT          358 a          706 c          725 g          417 t
ORIGIN      
        1 ccagagtcgc cgagcgaggc gagcgtgccg gccgctcccc cgagctgtgc ggccagcccc
       61 gactcgcgta cggagaagag ttgcctgcgg gcttcgagaa cgacgaccag ccagccggcg
      121 gccttgaggc cgaaggttgt cggaagagcc tgctgcagca gcgttcgccc ggccatgatg
      181 gttgcacggt gctcgtctgc gagccgcgcg cacgccgccg cgatcccatc gacctcgacg
      241 aggatgaggt ccacagccct acgcgcgacg agcatcgcgg cggtgtccac gatgtcctgg
      301 ctagtggctc ctttgtgtac gtagctaccc gcgtctccgg aggcttcgcc agaaacggcg
      361 gccgtgagcg cccgcaccag cggtgggacg gggttgcccg cggctcgccc cttccggccg
      421 atctcttccg gatcgaagcg gtctgcatcg cagtaccggg ctatcgttac ggcggcttcg
      481 tccgggatca agccggcccg cgcctcggca agcgccagcg ccgcctccgc atcgagcatc
      541 gcctgcagcc atgcgcggcc cccgagcgcc tcccggattt catccggcac gaagagcggt
      601 tcgaacaggt cctcagacat cgaagaatac ggtctgatgc tcgccctgca gattgatgtc
      661 aaagtgcagg tggccttcct gagaaccgcg ggctgtgaga gtcgagcgaa ggtcgtggtc
      721 ctcgatcgac gtgagaaccg gatcggcggc gttggcctcc tcctcatcgg ggaagtagat
      781 gcgggtcacg acttgcctga gcagaccccg cgcgaagatc gcaacgtcga tgtgcggtgc
      841 ctggggcagt ccatccgggc cgggtacggc gcccggtttg accgttacga actcgaaccg
      901 tccctcggta tcggtttggc agcgaccgaa gccggtgaag ccttcctcaa ggggaagctc
      961 gtcccggtga tcttccgggt gtgcgtagcg gccgttgctg ttcgcttgcc agatctccac
     1021 catagcgtcg gtgacgggct cgcctgcgcc gtcgtgcacc gtgccttcga tccggacggc
     1081 ccgagggtga tcgagtgcga cgagtctcga ccgatcctcc tcggtgaggg cgaagtgaaa
     1141 gaagggaccg acagtctgcg agggtgtggg ctctagccgg ggctcgggcg tcacttgtga
     1201 ttcctttccg tcggcgtcgc ctcgcgcccg cgaagaacga tgtcgaagcg gtatgccagc
     1261 gcccattccg gctcggtcgc ctccaggtcg aaggacgaga tcaatcgttc ccgcgctttg
     1321 gggtcacgca ccgagcggaa gatggggtcc tggtcgaaga gtggatctcc tgggaagtac
     1381 atctgggtga cgagacgggt cgcgaaggca ggcccgaaga gggagaagtg gatgtgggct
     1441 ggccgccagg cgttgtagtg gttgccccaa gggtaggcgc cgggttttac tgtcacgaac
     1501 cggtaacggc cttcgtcgtc ggtcaggcaa cgtcccgccc cgctgaagtt cgggtcgagc
     1561 ggcgccgggt gtctatcgcc ctggtggatg tagcgcccgg tggcgttggc ttgccagatc
     1621 tccacgaggc tgttcctgac cggccggccg tcgccgtcga gcaccctgcc gtgcacgatg
     1681 atgcgctctc ctaaaggctc ccctttgtgc tggcgcgtca ggtcgtgatc caacgagcct
     1741 accggctcgt gaccgtagac ggggccagtc atctccgtga gtgtgtgggg caagatgatg
     1801 agcggcttct ccggcgcccg caggcgtgtg cccttgtagt cgggatatag gtaaggtgga
     1861 tgaacctcag catcatcgcg ccgatacccg gcgactccgc tcctcgattg ctgtgccata
     1921 cgaggtgctc ttctcattca gaccttctcg acgaccatgg cgattccctg tcccacccct
     1981 atacacatgg tggcaagtcc cagctccacg tcttctcggc gacgcatctc gtgcagcaat
     2041 gtcgtaagaa tcctggctcc cgagcaccct atcgggtgcc cgagcgagat tgctccacca
     2101 ttcaggttca atctctcgtt gtccgggtcc atcccccact cgtagagcac tgccagcgat
     2161 tgggccgcga aggcctcatt gagctcgatc aggccaatat cgtcca
//
LOCUS       DATMVF010000175         5729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_614709, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000175 DATMVF010000000
VERSION     DATMVF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614709"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VN178_08685"
     CDS             complement(<1..622)
                     /locus_tag="VN178_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS81087.1"
                     /translation="MIEFRQVGKTYPGSQSPVVTDLSFEVLEGEICVLVGPSGCGKTT
                     TMRMVNRLIEITEGEILIDGEPNTAMSATRLRRKIGYAIQQIGLFPHRTIADNIGTVP
                     HLLGWNKDRIRDRVDELLELVGLHPGEYRDRYPAELSGGQQQRVGVARALAADPPIML
                     MDEPFGAVDPITRERLQDEFLRIQEDIKKTIVFVTHDIDEAIKMGNK"
     gene            complement(898..1173)
                     /locus_tag="VN178_08690"
     CDS             complement(898..1173)
                     /locus_tag="VN178_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81088.1"
                     /translation="MGLVRTILVLVILLILTHVALMYAGVGQGTNVLTNAVYSLGTLL
                     ESPAAFLLQSITVLQNYLDPSSFYVVAISAAALYFIIYLLLGVGRRA"
     gene            complement(1416..1802)
                     /locus_tag="VN178_08695"
     CDS             complement(1416..1802)
                     /locus_tag="VN178_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWS81089.1"
                     /translation="MALAASYIRTESEGGLPTEDEQMAAIMAYAAENGHDLVAHYKDL
                     DATGHLLYHRPGLKEAINNIKELEDWEVLVVAQPRCVSDTDSALHEFVHKLSLYGNRL
                     ESPARPWDDFLADMRSYRRAMSRGRL"
     gene            1863..1936
                     /locus_tag="VN178_08700"
     tRNA            1863..1936
                     /locus_tag="VN178_08700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1897..1899,aa:Pro,seq:ggg)
     gene            2144..2905
                     /locus_tag="VN178_08705"
     CDS             2144..2905
                     /locus_tag="VN178_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HWS81090.1"
                     /translation="MISSLEDLLLVDLGCLILHEAHDEDRLVSLRERIEAEREQRNPV
                     IVSPHEDRYLVLDGAHRVRALGELGSRFALAQVVEPPEKAEGWGHLLNGVGRSELDYI
                     EGIEVSDRFSDVSLAEVETSGGKIVILSAREDGLRGRVRALWDLQTIYPRGAVVRRVE
                     PDGSARLAGGEALIRYRSFTPGELAEIVDSGTVLPAGITRFRIQERVLGVRYPLDRMM
                     GGDPRARNAELKEFVERRWEENRVRYYGEPVVLFE"
     gene            3048..3596
                     /locus_tag="VN178_08710"
     CDS             3048..3596
                     /locus_tag="VN178_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81091.1"
                     /translation="MYRAAALMLALVVVVFFAAQLAEEPVRALQKPDVRPAPEAFEAA
                     GASGEGDVAGPTPDRGGLKIGSFEAPQRVPDYEVLEESFDTRDGARAARLLVDTRSRG
                     EEEYVPIARDVKHRYSDYDAISVEFTDTEDVLFYNDDPLTKDLLVHYGGALIFNTYEG
                     AYYLGYIYGPPNMEGYYVKAVD"
     gene            3851..5095
                     /locus_tag="VN178_08715"
     CDS             3851..5095
                     /locus_tag="VN178_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564534.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HWS81092.1"
                     /translation="MVFGRRARQALEMRRSGKSKAEVEKETGSTIGVYDAHFEVQPGE
                     IFVVIGLSGSGKSTLLRVINRLIEPTEGEVYLDKDHISQMPSKQLRQVRRRKIGMVFQ
                     HFGLLPNRTVLDNISFGLEIQGVSAEERRKSAEETLDMVGLQGQGNKLITELSGGMKQ
                     RVGLARALATEQEILLMDEPFSALDPLIRRDMQNLFLDIQGEVKRTVVFVTHDLDEAL
                     TLGHRVAIMKDGEIVQLGTPEHILREPANEYVERFVEDVDYAKVRQAESVMVEAKEVA
                     YINEGPTVVLRRMRQAGLSHMFVLDNERRLVGLCEAEEVARLAKRDERELRAAVDDDT
                     PKVQRRTPLREVLPLFVEDGDAPVAVVGESGRLQGVIVRGALISGLTTSVEPHDEGGG
                     DFSGVGTSGAGEDQVHARRAEE"
     gene            5099..5359
                     /locus_tag="VN178_08720"
     CDS             5099..5359
                     /locus_tag="VN178_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81093.1"
                     /translation="MLAFLQSELIPSIPLAEWTESVVDWITDTFDGAFDVLDTAIDLV
                     VGSLEYVLLAPPALLMVAILGVIAFSWRAGGWRCSPYWAWCS"
     gene            5323..>5729
                     /locus_tag="VN178_08725"
     CDS             5323..>5729
                     /locus_tag="VN178_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWS81094.1"
                     /translation="MALFTVLGLVFIISLDLWEAAMQTLALVLAATVVALIIGIPIGI
                     IAAKSGVVETAIRPILDIAQTMPAFVYLVPIVVILGIGPGPALIATVIFAMPPAVRLT
                     MLGIQQVPKDTVEAAHAFGATPWQTLFKVELPLS"
BASE COUNT         1056 a         1727 c         1837 g         1109 t
ORIGIN      
        1 tcttgttccc catctttatg gcctcgtcta tgtcgtgggt gacgaacacg atggtcttct
       61 ttatatcttc ctgaatccgc aagaactcgt cctggagccg ctctcgcgtg atcggatcga
      121 cagccccaaa gggctcgtcc atcagcatga tcggggggtc agccgccagc gcccgcgcga
      181 cccctacgcg ctgctgctgg ccgcccgaga gctcggccgg gtaacggtcg cggtactcgc
      241 caggatggag gccgacgagc tccaggagct cgtccacgcg gtccctgatg cggtccttgt
      301 tccagccgag caggtggggg acggtaccga tgttgtcggc gatggtgcgg tgggggaaga
      361 ggcctatctg ctggatggcg tagccgatct tgcgcctcag ccgggtggcg ctcatggccg
      421 tgttcggctc gccgtcgatg aggatctcac cttcggtgat ctcgatcagg cggtttacca
      481 ttcgcatggt cgtggtcttg ccgcagccgc tgggtcccac caaaacacag atctctcctt
      541 cgagcacttc gaaggagaga tccgtgacca ccgggctttg ggagccgggg taggtcttgc
      601 ctacctgcct aaactcgatc attgcacctc ctgcgtgccc agatgtggta ctcgctcttt
      661 ctgagctcac ttatctttac cgcttcgatc atagctcttc tcttaaggat ccggaatgca
      721 ctcgaagaag tatacgttat tgtcgttacc ctggttcaca gcgccgggca gaacactccc
      781 tagccttctg cccggcgcgc tttcagtcaa cagctgtcag tcgtcagcca aaacaagaag
      841 ctgatagctg aaagctgacc gcgcgattgg cgggggccat cggccccgct aatcgctcta
      901 cgcccggcgg ccgacgccga gcaagaggta gatgatgaaa tagagcgcgg cggccgagat
      961 ggcgacgacg tagaagctac tgggatcgag gtagttttgc aggaccgtta tggattggag
     1021 caggaaggcg gcaggggact cgagcaacgt ccccaggctg tagacggcgt tcgtcaatac
     1081 attggtgccc tgaccgactc ccgcgtacat cagcgctacg tgggtgagta tgagcaggat
     1141 gacgaggacc agtatggtac ggacgagacc catgcctcta actccttccc ttcgcgacca
     1201 acgctgttac gccctggatg gctgcaaggg ttcgtccggc catatcgcac cggactctct
     1261 cctccccatg accctcctca gggccgttct cagcgggcag tatagcaata acgctgcttg
     1321 ggagaggccc cgcgggcagt ctaggatgcg gcgctctctg aatccctggc ctgcgcgttt
     1381 cacattcaag ggtcgttcgc aaacgacccc tacactcaca aacgacccct actcatcgca
     1441 cggcggtagg acctcatatc tgcgaggaaa tcgtcccagg ggcgtgccgg agactccaga
     1501 cggttcccgt acagggagag cttgtgtacg aactcgtgca gggccgaatc tgtgtcggag
     1561 acgcaccgcg gctgtgcgac cacgagcacc tcccagtcct cgagttcctt tatattgttt
     1621 atcgcctcct tgagaccggg gcggtggtag agcaggtgtc cagtggcatc gaggtcttta
     1681 tagtgggcga cgaggtcgtg accgttctcg gcggcgtagg ccataatagc ggccatctgt
     1741 tcgtcctctg tggggagccc gccctcggat tctgtgcgta tgtaggatgc agccagcgcc
     1801 acggagatcg catgatagca tgggtctcgt ctggtatgat cggggtctga tcacagacgc
     1861 gtcgggacgt ggcgcagtct ggtagcgcac tcggctgggg gccgagtggt cgccggttca
     1921 aatccggccg tcccgactgt ttgcggtgaa accttaaggc aagcgggcat ccgttgaaag
     1981 gcgagctttg gatacgatct atctgcttct tggcatcttg gggttctttc tcatcatcgc
     2041 gctcttcttc ttcggcggtt tcgtcttgct cgactttttg tggggccgcc gcgggggaga
     2101 cgcctagagt cctggcggtc tcgtcccgcc gcatgcctgg tacgtgatct cctccctcga
     2161 agaccttcta ctcgtagacc tcgggtgtct gatcctccat gaagcccacg atgaagaccg
     2221 cctggtcagc ttgagggagc gcatcgaagc cgagcgtgag cagcgcaatc ccgtaatagt
     2281 ctcgccgcac gaagaccgct acctcgtcct cgacggggcc cacagggtac gcgccctcgg
     2341 agagcttggt tcccgcttcg ccctcgccca ggtggtcgag ccgccggaga aggcggaggg
     2401 ctggggccat ctcctgaacg gggtcggaag gtccgagttg gactacatcg aaggaatcga
     2461 agtctccgat agattcagcg atgtttccct ggccgaggtg gagacctccg gcggcaagat
     2521 cgttatcctt tcggccagag aggatgggtt gcgaggacgg gtacgggcgc tctgggatct
     2581 gcaaacgatc taccccaggg gcgccgtagt gcggcgcgtg gaaccggacg gttcggcccg
     2641 tctcgcgggt ggcgaggccc tgatccgtta caggtcgttt accccgggag agctggccga
     2701 gatcgttgac tcgggtaccg tgcttcccgc cggtataacc cgattccgca tccaggaacg
     2761 ggtcctaggc gtacgttacc cgctcgatag gatgatgggg ggagaccccc gcgcccgcaa
     2821 cgccgagctc aaggagttcg tcgagaggcg ctgggaagag aaccgcgtac gctactacgg
     2881 agagcccgtc gtactcttcg agtgaatgaa agcatttcag ccagtcagca cttcagcatt
     2941 tcagctactt gttctcgcta atggtcgaat tcacaattcg tggtaggacg atacttcgtg
     3001 tacatcgggt accatagttt cgtcctcagc gtgaaggaga gtcccgcgtg taccgtgcag
     3061 cggccctgat gctggccctc gtcgtcgtgg tcttctttgc ggcacaactc gccgaggagc
     3121 cggtgcgggc cctccagaag cccgacgtgc gcccggcacc cgaagctttc gaggcggcag
     3181 gggcctcggg tgagggagat gtggccgggc caactcccga cagggggggc ctgaagatcg
     3241 gaagcttcga ggcgccgcag cgcgttccgg actacgaggt cctcgaggag agcttcgaca
     3301 cccgcgacgg cgctcgcgcg gcgcggctcc tagtcgacac ccgctcgcgc ggggaggagg
     3361 aatacgtccc catcgcccgc gacgtaaaac accgctactc cgactacgac gccatctcgg
     3421 tagagttcac cgacaccgaa gacgtgctct tctacaacga cgacccgctc accaaagacc
     3481 ttctcgtcca ctacggtggc gcgctcatct tcaataccta cgagggcgcc tactacctgg
     3541 gctacatcta cggcccgccc aacatggaag gttactacgt gaaggccgtc gactagcgcc
     3601 ccgcgcgccg gaacgtgtag acaaccgccg cttgccttgc cttacagttg aagggttgcc
     3661 ggaaagcgca tatcatacag aatgcagccg gaaggaaagg gtggttgcat gcattccatg
     3721 tcgtgtccgg cggcggtggt gtaagcgctg gaccctgcaa ccttttgtag aaggctagtg
     3781 aaggagagag gccacggaag ctcacgagat tgagggccag gacccgatca tccgggtaga
     3841 gaaatgttcg gtggtcttcg ggcgtcgagc ccggcaggcc ctggagatgc ggcgttcggg
     3901 aaagagcaag gccgaggtcg agaaagagac cggatctacc atcggcgtct acgacgcgca
     3961 cttcgaggtg cagccgggcg agatcttcgt ggtcatcggg ctctccggga gcggcaaatc
     4021 gacgctcttg cgggtgatca accgcctcat cgaaccgacc gagggcgagg tctacctgga
     4081 caaagaccac atctcccaga tgccgtccaa acaactcagg caggtccggc gcaggaagat
     4141 aggtatggtc ttccagcact ttgggctact gcccaaccgg accgtgctag acaacatctc
     4201 cttcggcctg gagattcagg gcgtctccgc cgaggagcgc aggaagagcg cggaggagac
     4261 cctggacatg gtcggcctcc agggccaggg caacaagcta ataaccgagc tctccggcgg
     4321 catgaagcag cgggtgggtc tcgcgcgcgc cctggcgacc gagcaagaga tactactcat
     4381 ggacgagccc ttctcggcgc tcgaccctct gatccggcgc gacatgcaga acctctttct
     4441 ggacatccag ggcgaggtca agcggacggt ggtcttcgtc acgcacgacc tcgacgaggc
     4501 gctcacgctc gggcaccggg tggcgatcat gaaggacggc gagatcgtgc aactgggcac
     4561 cccggaacat atcttgaggg agccggcaaa cgagtacgtg gagcgctttg tcgaggacgt
     4621 ggactacgcc aaggtgcggc aggccgagag cgtgatggtc gaggcgaagg aggtcgcgta
     4681 catcaacgag ggtcccacgg tcgtcctgcg gcggatgagg caggccggcc tgtcccacat
     4741 gttcgtactg gacaatgagc ggcgcctggt gggtctgtgc gaagccgaag aggtcgccag
     4801 gctggccaag cgtgacgagc gtgaactccg ggccgcggtg gatgacgaca ccccgaaagt
     4861 tcagcggaga accccgctgc gcgaagttct gccgctcttc gtggaggacg gggacgcgcc
     4921 cgtggccgtc gtgggcgaaa gcggacgcct ccaaggcgtt atcgtccgag gcgcgctgat
     4981 ctctggactg accacgagcg tcgagccgca cgacgaaggt ggcggtgact tcagcggcgt
     5041 tggcaccagc ggtgccggcg aagatcaagt ccacgcgcgc cgggcagagg agtagaagat
     5101 gctggctttc ttgcagtcgg aactgatccc gagcatacca ctggccgagt ggaccgagag
     5161 cgtggtcgac tggatcaccg acaccttcga cggggctttc gatgtgctgg acaccgcgat
     5221 agatctcgtc gttggctcgc ttgagtacgt cctcctcgcg cccccggcgc tgctgatggt
     5281 cgccatcctg ggcgtgatcg ccttctcctg gcgggctggt gggtggcgct gttcaccgta
     5341 ttgggcctgg tgttcataat cagcctggac ctgtgggagg ctgcgatgca gaccctggcc
     5401 ctggttctgg cggcgacggt cgtcgcgctg ataataggca tcccgatcgg tatcatcgcg
     5461 gccaagtcgg gcgttgtcga gacggccatc cgtcccatcc tcgacatcgc ccagacgatg
     5521 ccggccttcg tgtacctggt ccccatcgtc gtgatcctcg gtatcgggcc aggacccgcc
     5581 ctgatcgcga cggtcatctt cgccatgcct cccgccgtga ggctgacgat gctaggcatc
     5641 cagcaggtgc ccaaggacac cgtggaagcc gcgcacgctt tcggtgcgac cccctggcag
     5701 acgctcttca aggtggagct tcccctgtc
//
LOCUS       DATMVF010000176        11966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_119483, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000176 DATMVF010000000
VERSION     DATMVF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119483"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..985
                     /locus_tag="VN178_08730"
     CDS             77..985
                     /locus_tag="VN178_08730"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWS81095.1"
                     /translation="MAGGASPTKGQIRWDAHRRAFEEYLHGLNFTVEPRLSGLEEAMR
                     YSLLAGGKRVRPALCMETAWIFGAEPSHVLPSAAAIELIHTYSLIHDDLPAMDDDDYR
                     RGRPTAHKKFGEAMAILAGDAFFGEALTLITVHQKGSCEEILEVVRELADSTGVNGMV
                     GGQVLDMDHTGLAGATEPETLYMIHEYKTGALIKSSARIGSILAGATGEEQAAVSEYA
                     SQLGLCFQIVDDVLNATSTREELGKSAGSDAEQGKATFAGVFGLEGARQEADEAARRA
                     ITALSRVDGDTSGLQELAYFVRHRER"
     gene            995..1798
                     /locus_tag="VN178_08735"
     CDS             995..1798
                     /locus_tag="VN178_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWS81096.1"
                     /translation="MESSPKGSPKRLDALLVEKGLAASRSSARGMILAGSVRVGDAVV
                     TKAGSRIGADERLFVERPERFVSRAGEKLDAAIGEFGVRVEGRSCLDAGASTGGFTDA
                     LLQRGADRVLAVDVGYGQFDWKLRNDPRVTVMERTNVRRLAGEDLPFQPDLLVADLSF
                     ISLTVALQSIFSTTPALREAILLVKPQFEAGPESVGRGGLVRDREVHAAVIRSVARDF
                     GTLGFGAVGATRGAVAGRKSGNLEYPVHLIRGAEQDLDEARISEVVGGG"
     gene            1791..2600
                     /locus_tag="VN178_08740"
     CDS             1791..2600
                     /locus_tag="VN178_08740"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWS81097.1"
                     /translation="MGNIMNAARIRRVGIVANPRVSDGYVERLVAVLEKNRVEVSEVK
                     GDDQPDGARDIDLVFVLGGDGTMLRASRMYPGRVLLGVNLGKVGFMSGMLPEELETGV
                     EKVLGDGLEVQEYRMLDVRVGKEEPGLAANDAVLIKKRPHQLISVDVSIGGEELFAFR
                     CDGFIAATPLGSTAYALSAGGPIISGDAGCYVLVPIAPHALVSRPLVLGEDQVAELAL
                     VERDALLSLDGAEPREIPAGGSVEIRLSTESVRIGRTEDWSWWRAVRRTFL"
     gene            2570..4267
                     /gene="recN"
                     /locus_tag="VN178_08745"
     CDS             2570..4267
                     /gene="recN"
                     /locus_tag="VN178_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWS81098.1"
                     /translation="MASSSEDVPVVLTELSVENVALIEEATLELSPGLNVITGETGAG
                     KTLLATALQMLLGGRARSETVRTGARKATVEGTFVLPEESGSRFLAEALGDLAEEVEE
                     DDGLVLRRTLTGEGRSRCYVGGVSVPVRSLASLGEKLVSYHSQREQIRLTEPAEQLAI
                     LDDFLDERASGARHEHGQIWRAVESDRAALREIKTTSEARLREVEFLRYQVSELESAG
                     YDVQELTDLSRERDRLRNVTDLLQATAAATTALSSEDGTGAVDAVGRASSELETASHY
                     DESLVGLLKRLGGLSAELDDVLYELRSYVDELEADPNRLEAVEDRLAAMRALERKYGD
                     VEGYLQESRERLSRLENLDEETAELEERLAEGEQRLEVLATSLRSGREAAAERLSGKV
                     QENLEDLNLGKTTFRAELVPTEPGPSGTEGVEFVIQPNPGEPELPVRRYASGGELSRI
                     MLAIRLAQERLEPGATYIFDEVDAGIGGETATAVGAKLRELGDINQVLTITHLPQIAS
                     EASTHVVVEKEESKGRTITRVERIEGEERRRELARMLSGRIDEASLAHASELLVGGR"
     gene            4334..6391
                     /locus_tag="VN178_08750"
     CDS             4334..6391
                     /locus_tag="VN178_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWS81099.1"
                     /translation="MGISGDIALILVAALLGGVVARWLRLPLILGYIAAGVLVGPNTF
                     GPSVGDVHEIELLAEIGVALLLFTIGLHFSLAELAPVRRIALLGTPVQMALTIAFGYG
                     VGRLLLDLGWVESVWLGALFSLSSTAVVLKTLGEQGVLGTLSARVMIGMLIVQDLAIV
                     VLLTVLPVLENVKEGLPELGLAVLKAAAFIAAMLLLGSRVLPMFLARLAAWGSRELFL
                     VSVVAIGVGVGYATYLVGLSFAFGAFVAGMVLSQSDYSHQALAEVEPLRDVFAMIFFV
                     SVGLLIDPSYLWANFGTIALVVALILVVKGLIFGSLTRVFGYGNIVPFAVGLGLFQVG
                     EFSFLLAREGISADSISQGTYSLVLASAAVTMALTPLVARLAPILYGRYRRRFPREPL
                     QTFNLPAGGLTDHAVVAGYGRVGSFVARLLDRLGKPFVVVEANPNRADQAKAAGFPTV
                     YGDAVTIPVLEAAGTGGARLVVLTIPDALATRLAVERLRNLAPEADLLVRAESAGQLE
                     DLGRLGVYEAVQPELEAGLELARQALARFGVAAEEAQTFADGVRRELYAPISGEEAAR
                     EGGDSLFARLRQASQAIEVEWVRLAEGRTPEKDGEESPTGHGLIGHTIGDLGVRSETG
                     ASILAIVRGDEVIPNPGADLALQAGDAVGVLGTREQRAAFRSIAGVNADESPEVTQGL
                     SDR"
     gene            6576..8051
                     /locus_tag="VN178_08755"
     CDS             6576..8051
                     /locus_tag="VN178_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWS81100.1"
                     /translation="MRTKSVEQSIRDTEDPEFQLRKALGPLDLTLFGIGVIIGTGLFV
                     LTGEAAAGYAGPAVALSYVVGGIVCALAALCYAEFASTVPVAGSAYTFSYATLGEFVA
                     WLIGWDLILEFTLGAATVAKGWSGYLVSVFEILGISLPTSLYASPESGVSHDWLALIV
                     VALVTTILVIGIRLSSQFNAVITSIKILVTLFIIGFGIFFISTANLTPFIPPPQSGGE
                     ELQVYLLPSILGIDTIYGITGIFTAAALVFFAYIGFDIVATTSEEARNPQRDLPIGIL
                     GSLGICTVLYILASIVFTGLVPYDTLATAAPAATALEATPFPAAQLIVSLAILIGLTV
                     VVMILVLGQSRVAFAMSRDHLLPPWFSQVHRSFRTPHRITIITGIVAGLLAFFLPLVT
                     LGELVNIGTLAAFVLVSIGVMILRRTRPDLPRAFKVPGYPVVPILAAISCLFLMGFLT
                     TGTWLRFLVWMAIGLVIYFTYSKNHSRLANEDSSAESSQEA"
     gene            complement(8106..8438)
                     /locus_tag="VN178_08760"
     CDS             complement(8106..8438)
                     /locus_tag="VN178_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81101.1"
                     /translation="METHPIQVTPKYKAYRIVVRAEIGERFAAAFEGMEVRIAEGRTI
                     ITGEVIDQSHLHGILDRINALGLVLVSVQPESEEPPGGSPASHWRRTGPAEERTILED
                     RYKRKGDR"
     gene            complement(8587..10839)
                     /locus_tag="VN178_08765"
     CDS             complement(8587..10839)
                     /locus_tag="VN178_08765"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS81102.1"
                     /translation="MSDSVSPDSSAVIGTKLRVPSPRPEQVIRPRLFEMLEAGLERKV
                     TLVSAPAGYGKTILLSQWLMSDETNGAFAWVSLDEQDNDPIRLWRHIVESLGRGVPVK
                     AFGASALAALNVAGAGVVDTVLPRLVNELGEVPQKVTLVLDDYQFATDDGCRESMEFF
                     VEHLPENFHLVLATRSDPPLRLGRLRARGELAEIRTEELAFTEREAAFLLNGRLHLEV
                     GTDDLRVLLVRTEGWPAGIYLAALSMEGKENVHDFIQAFGGSNRYILDLLGEEVLAGL
                     PAAEREFMLKTSVLAEMAGPLCDEVVGGESSGALLDELARSNLFVVALDENGGWYRYH
                     HLFSDLLLYELKSSRPDLLPELHGRAGAWFEEKGMFEDAIRHAMAASEHQRAGMLIAR
                     HWFRYAVTGQLSSLERWLEALPEYLRGRDAPLVLVRAWICAIYGRREESEHFLRLAES
                     LPYEGRLPDGSTAVESETAIIRALFGYGGVKSMVAAAARSVELESEQDPPWRLALVKM
                     ALGQSAYLSGDISTARKSLDDAIAMISADQPLWRIGALYLLSVVAVDEGRLDEAESHA
                     REARSLVERFIPQGSRETSAVSIALGRVFGELGRLDEAQTELESGLAARRSPDVNPWP
                     TLLGLLALARVRAARGDRPGALVLLEEARDIVEAYPDAGIFPDLLERQEHELSKSRRV
                     ESALTEVLTERELDVLRLFDGGDSYRQIGQALYVSVNTVKTHARSIFRKLEVSSRDEA
                     LERARQRALI"
     gene            11169..11738
                     /locus_tag="VN178_08770"
     CDS             11169..11738
                     /locus_tag="VN178_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HWS81103.1"
                     /translation="MADVAQPQPMLSDAAGRVWWTLVLRGVVAVLFGLAALFWPGKTL
                     FVLILFFGAYTLVDGIFAIVGGIMDSSRRWLLLIEGVLGVVAGLILLAWPGLGALVLL
                     YVIAIWAVVTGVMEIIAAISLRREIDNEWLMILGGAISVLFGVILAVLPGAGLLSLTW
                     LIGAFALVFGIAFIVLGFKVRRLDSSRVS"
BASE COUNT         2088 a         3846 c         3871 g         2161 t
ORIGIN      
        1 tccggtgacg aagcgccgtc tttcgtgggg ggagtcctac gaggcgccga gctgagagcg
       61 cagggcaagg ttgggcatgg cgggcggggc tagcccgacg aaaggccaga tcaggtggga
      121 cgcccacagg cgcgccttcg aggagtacct gcacgggctc aacttcacgg tagagccgcg
      181 gctcagcgga ctcgaagagg ccatgaggta ctctctgctc gccggcggaa agcgtgtgag
      241 accggccctt tgcatggaga cggcctggat cttcggggca gaaccatccc acgtgctccc
      301 ttccgcggcg gccatcgagc tcatccacac ttactcgctc atccacgacg accttcccgc
      361 gatggacgat gacgactaca ggaggggaag gccgacggcc cacaagaagt tcggcgaggc
      421 gatggccata ctcgctggcg acgccttctt cggcgaggca ctgaccctga tcacggtcca
      481 ccagaaaggg agctgcgagg agatcctgga ggtggtgcgg gagctcgccg actcgacggg
      541 ggtcaacggc atggtcggcg gacaggtctt ggacatggac cacaccggcc ttgccggggc
      601 gaccgagccc gagaccctgt acatgatcca cgagtacaaa acgggtgccc tgatcaagtc
      661 ctcggcccgt atcgggtcga tactggcggg ggccaccgga gaggaacagg ccgccgtctc
      721 ggagtacgct tcgcagctgg gcctctgctt ccagatagtc gacgacgttc tcaacgctac
      781 gtcgacgcgg gaggaactcg gcaagagcgc cggttcggac gccgagcagg gcaaggccac
      841 gttcgcgggc gtcttcggtc tcgagggagc gagacaagag gcggacgagg ccgcacggag
      901 ggccatcacc gcgctctcca gggtcgacgg agatacctcg ggtctgcaag agctggcgta
      961 tttcgtgcgc cacagggagc gttagtcctc aggcatggag agtagcccga aagggagccc
     1021 caagcgattg gacgccttgc tcgtcgagaa gggcctcgcc gcgagcagga gcagcgctcg
     1081 gggtatgatc ctcgcgggca gcgtacgcgt tggcgacgcg gtagtgacca aagcgggctc
     1141 gcgcatcggc gccgatgagc gcctctttgt cgagcggccc gaacgcttcg tatcgcgtgc
     1201 cggcgagaaa ctggatgcag ccatcggaga gttcggcgtc cgggtagagg gacgttcctg
     1261 cctcgatgcg ggagcatcga cggggggatt taccgacgct ctcctgcaac gaggagcaga
     1321 tagagtgctc gccgtcgatg tgggctacgg ccagttcgac tggaagctac gcaacgatcc
     1381 acgcgtcacc gttatggagc gaaccaacgt gcgccgcctg gccggagagg atctcccgtt
     1441 ccagcccgat ctcctggtgg cagacctctc tttcatctcg ctcacggtcg cgctacagag
     1501 catcttctcg accactcccg cgctgcggga ggcgatcctg ctagtcaagc cccaattcga
     1561 ggcgggaccc gaatcggtag gacgcggcgg cctggtgagg gacagggagg tacacgccgc
     1621 cgtgatacgc agcgtcgccc gagacttcgg taccctcggt ttcggcgccg tcggggcgac
     1681 acggggggcc gtcgcaggaa ggaagtcggg gaatctcgag tacccggtgc acctgatcag
     1741 gggagcggag caagacctgg acgaggcgcg catcagtgag gtggtcggcg gtgggtaaca
     1801 tcatgaacgc cgcccgaatc cgcagggtag gcatcgtcgc caaccccagg gtgagcgacg
     1861 gctacgtcga gcggctcgtg gccgtcctcg agaagaacag ggtagaggtc tccgaggtca
     1921 agggcgacga ccagccggat ggtgccaggg acatagacct cgtcttcgtg ctcggcggcg
     1981 acgggaccat gttgcgggct tcgaggatgt accccgggcg ggtgctgctc ggggttaacc
     2041 tcgggaaggt tggattcatg tcggggatgc tccccgaaga gctggagaca ggcgtcgaga
     2101 aggtcctggg agacgggctc gaagtccagg aatacaggat gctcgatgtg cgggtcggca
     2161 aagaggagcc aggcctcgcg gccaacgacg ccgtgctcat caagaaacgt ccccaccagc
     2221 tcatctccgt cgacgtctcc atcggcgggg aggagttgtt cgcctttcgg tgcgacggct
     2281 ttatcgcggc gacccccctc ggctcgaccg cctacgcgct ctctgcgggc ggcccaatca
     2341 tctcgggcga cgccggttgc tacgtgctcg tccccatagc gccgcacgcg ctggtgagca
     2401 ggcccctggt gctgggcgag gaccaggtcg ccgaactggc gctcgtcgag cgggacgcgc
     2461 tgctctcgct ggacggggcc gagccgcgtg agatccccgc cggcggaagt gtggagatca
     2521 ggctctctac cgaaagcgtt agaataggcc gtaccgaaga ctggagttgg tggcgagcag
     2581 ttcggaggac gttcctgtag tgctcacgga gctctccgtg gagaacgtgg ccctcatcga
     2641 agaggccacc ctcgaactct cccccggcct caacgtgatc accggtgaga cgggggcggg
     2701 caagacgctc ctcgccaccg ccctgcaaat gctcctcggc ggccgcgccc gctccgaaac
     2761 cgtccgtacg ggcgcccgca aggccaccgt cgaaggcacg ttcgtcctcc ccgaggagtc
     2821 gggatcccgg ttcctcgccg aagccctcgg cgacctcgcc gaagaggtgg aagaggatga
     2881 cggcctcgtc ctcaggcgga cgctgaccgg ggagggccgc tccaggtgct acgtcggcgg
     2941 tgtctcggtc cccgtaaggt ccctggcgtc tctgggcgag aagctggtct cgtaccacag
     3001 ccagcgcgag cagatacgcc tcaccgagcc ggcagagcaa ctcgccatcc tcgacgactt
     3061 cctggacgag agagcctctg gggcgagaca tgaacatggc cagatctggc gcgccgtgga
     3121 gagcgaccgc gccgcgcttc gggagataaa gacgacctct gaggcgcggc tgcgggaggt
     3181 agagttcctc cgctaccagg tctctgagct cgagtcagcg ggctacgatg tgcaggagct
     3241 cacggacctc tcccgcgaac gcgaccgtct aaggaacgtg acggacctcc tccaggccac
     3301 agccgccgca acgactgccc tctcctccga agatggcacg ggagccgtag acgccgtcgg
     3361 gcgggcctca tcggaactgg agacggcctc gcactacgac gagagcctgg tcgggctctt
     3421 gaagcgcctg ggcggtctct ctgcggagct ggacgacgtg ctctacgagc tcaggtcata
     3481 cgtggacgaa ctcgaggctg acccgaaccg gttggaggcc gtagaagacc gcctcgccgc
     3541 catgcgggca ctggagcgta agtatgggga tgtcgaagga tacctccagg aatcgcgcga
     3601 acggctctca cgcctcgaga acctggatga ggagaccgca gaactcgaag agaggctcgc
     3661 ggaaggcgag cagcgcctcg aagtcctcgc gacctcgctg cgctcgggca gggaggccgc
     3721 cgcagagcgg ttgtcgggga aggtgcagga gaacctcgag gacctgaacc tcgggaagac
     3781 gaccttccgc gccgagctcg tgcccacaga acccggccct tctggcacag aaggtgtgga
     3841 gttcgtgatc caacccaacc cgggagagcc cgaactgccc gtcaggcgct acgcatcggg
     3901 cggggaactc tccaggataa tgctggccat aaggctcgcc caggagcgcc tggagccggg
     3961 tgcgacatac atcttcgatg aggtcgacgc cggtataggc ggggagacgg caacggcggt
     4021 gggggcgaag ctgagggaac tcggcgacat caaccaggtc ctgaccatca cccacctccc
     4081 tcagatagcc tcggaggcaa gcacgcacgt cgtcgtcgag aaagaggaat ctaaagggcg
     4141 gacgatcacg cgcgtagagc gcatcgaggg cgaggagcgc cgcagggagc tcgccaggat
     4201 gctctcggga cggatcgacg aagcctccct cgcccacgcc tccgaactcc tggtcggcgg
     4261 aagatagagg ccctccgtag gacccatcgt cgccggcggt gctcttccac ggtggtaaag
     4321 tgagagccag cctttgggga tctccggcga catagcgctc atcctggtgg ccgccctcct
     4381 ggggggcgtg gtggcccggt ggctcaggct gcccctgatc ctcggctaca tcgccgccgg
     4441 cgtcctggtc ggtccgaaca ccttcggtcc ttcggtgggg gacgtacacg agatagagct
     4501 gctggcggag atcggggttg ccctcctcct gttcacgata ggacttcact tctccctggc
     4561 ggagctcgcg cccgtgcggc gcatcgcctt gctcggcacc ccggtccaga tggcgctcac
     4621 catcgccttc ggctacgggg tcggccgcct tttgttggat ctgggttggg tcgagtcggt
     4681 gtggctgggg gccctgttct cgctctcgag caccgccgta gtcctcaaga ccctgggcga
     4741 gcagggggtc ctcggcaccc tctcggcaag ggtgatgatc gggatgctca tcgtccagga
     4801 cctggccatc gtagtgctcc tcaccgtgct accggttctg gagaacgtaa aggaaggcct
     4861 tcccgaactc ggcctggcgg tcctcaaagc ggcggcgttc atcgccgcca tgctcctgtt
     4921 gggctccagg gtgctaccca tgttcctggc gcggctggcg gcctggggtt cacgcgagct
     4981 cttcctggtc tccgtggtcg ccataggcgt cggtgtcgga tacgcaacct acctcgtcgg
     5041 actctctttc gccttcgggg ctttcgtggc ggggatggtc ctctcccagt cggactacag
     5101 ccatcaggct ctggccgagg tcgagccact gcgcgacgtt ttcgccatga tcttcttcgt
     5161 ctccgtgggg ctcctgatcg acccttccta tctctgggcg aacttcggga ccatcgcgct
     5221 cgtcgtcgcg ctgatcctcg tcgtgaaggg cctgatcttc ggctctctga ccagggtctt
     5281 cggatacggc aatatagtcc cgttcgccgt gggactgggg ctcttccagg tcggggagtt
     5341 ctcgttcttg ctcgccaggg aaggcatcag cgcggactcc atttcccagg gaacctactc
     5401 cctcgtgctg gccagcgcgg ccgtcacgat ggccctcacc cccctggtcg cccgtctcgc
     5461 gcccatcctc tacggccgtt accggcggcg gtttcccaga gagccccttc agaccttcaa
     5521 cctgccggca ggagggctca cggaccacgc cgtcgtcgcc gggtacggtc gggtcggatc
     5581 cttcgtcgcc aggctactag acaggctagg gaagccgttc gtcgtcgtgg aggcgaatcc
     5641 gaaccgcgcc gaccaggcga aagcggcggg tttccccacc gtgtacgggg acgccgtgac
     5701 catacccgtc ctggaggccg ccggaaccgg tggggcgcgc ctcgtcgtgc tgaccatccc
     5761 cgacgccctc gcaacgagac tcgccgtaga acggctacgg aacctggccc ccgaagcgga
     5821 tctcctggtc cgggccgaga gcgccgggca gctggaagac ctcggccgtc tcggcgtcta
     5881 cgaggcggtg cagccggaac tggaggccgg cctggaactg gcgcgtcagg cgctcgcccg
     5941 cttcggtgtc gcggccgagg aagcccagac cttcgccgac ggcgtgaggc gcgaactcta
     6001 cgccccgatc tcaggggagg aggccgccag ggagggcgga gacagcctct tcgcgaggct
     6061 gcgccaggcc tcacaggcca tcgaagtaga gtgggtgcgg ctcgctgagg gacgcacccc
     6121 cgaaaaggac ggggaagaaa gcccaaccgg acatggcctc atcggtcata ccatagggga
     6181 cctcggcgta aggagcgaga caggggcatc tatcctggcc atcgtgaggg gtgacgaggt
     6241 gatccccaac ccaggggcag acctcgcact ccaggccggc gatgcagtag gcgtcctggg
     6301 cacccgggag cagcgggcag cattccgctc catcgccggg gtcaacgccg acgaaagccc
     6361 cgaagtcacc caaggtctgt ccgaccgctg aggtaagtca caggcctcgc cctctcgcac
     6421 cctggaacta tttcttccca taatactatg agtgcgggca tggaccctgt gcgagttgat
     6481 ctgtacggcg caagaaagga gaaacttgta gaatgtccgg tagcgtgggt gcaaggatcg
     6541 tgtaagggac ctgaaagggg agctacatgg ggataatgcg aacgaaatcc gtcgagcaat
     6601 ccatcaggga cactgaggat cctgagtttc agctacgcaa ggcgctggga cctttggatc
     6661 tcactttatt cggtatcggg gtcattatcg gtacggggct cttcgtcctg accggagagg
     6721 cggccgccgg ctacgccggg ccagcggttg cgctctccta cgtcgtcggc ggcatagtgt
     6781 gcgccctggc ggcgctctgc tacgcggagt tcgcttcgac cgtaccggtg gccggcagtg
     6841 cctacacctt ctcctatgcg accctcggag agtttgtcgc ctggttgatc gggtgggacc
     6901 tgatcctgga gttcaccctc ggcgccgcca cggtggcgaa gggatggtcg ggctacctgg
     6961 tgagcgtctt cgagatcctc gggatttcgc tgccgacgag cctctatgcg tctcccgagt
     7021 ctggcgtctc ccacgactgg ctggcgctga tcgtggtagc gctcgtgacg acgattcttg
     7081 ttatagggat caggttgagc tcgcagttca acgcggtgat aacctctatc aagatcctcg
     7141 ttacgctgtt tatcataggc ttcgggatat tcttcatatc gactgccaac ctcacgccct
     7201 tcatcccgcc gccgcagtcg gggggagagg agctccaggt gtacctcctg ccgagcattt
     7261 tgggtataga caccatctac ggcataacgg gcatcttcac ggcagcggcg ctggtcttct
     7321 tcgcctacat aggtttcgac atcgtggcca ccacctccga ggaggcgcgc aatccccaga
     7381 gagatttgcc catcggcatc ctgggctccc tggggatctg cacggtcctc tacatcctgg
     7441 cctccatcgt ctttacgggg ctcgtgccct acgacacgct ggccaccgcg gccccggcgg
     7501 ccacggcgct ggaggcgacg cccttcccgg cggcgcagct tatagtctcg ctggccatcc
     7561 tgattgggct cacggtcgtg gtgatgatcc tggttctagg gcagagccgg gtggccttcg
     7621 cgatgagccg ggaccatctg ctgccgccct ggttctcgca ggtccaccgc agctttcgca
     7681 cgccgcacag gatcacgatc attaccggca tcgtcgcggg cctgctcgcg ttcttcctcc
     7741 cgctggtgac gctcggggag ctcgtgaaca tagggaccct cgccgccttc gtgttggtct
     7801 ccatcggggt gatgattctg cggcggacgc ggcccgatct cccgcgggcg ttcaaggtac
     7861 caggctaccc cgtggtgccc atcctcgccg ccatctcatg cctcttcctc atgggtttcc
     7921 tgaccaccgg cacctggctg aggttcctgg tctggatggc gataggactg gtgatctact
     7981 tcacctacag caagaatcac agcagattgg ccaacgaaga ctcgtccgcc gaatcgagcc
     8041 aggaggcctg aacgtagccg gaccggggcg cttggcgccc cggtccgcat cccctgacaa
     8101 acctattatc gatccccttt ccttttgtat cgatcctcga ggatggtgcg ctcttctgcc
     8161 ggtccggtcc tcctccaatg acttgccggg ctgccccctg gcggttcttc ggactcgggc
     8221 tggacgctaa cgagcaccag tcccagggcg tttatgcgat ctaggatccc gtgcaggtgg
     8281 gactggtcga taacttcacc cgtaatgatc gtccgtcctt ccgcgattct cacctccatc
     8341 ccttcgaagg ccgctgcgaa tcgctcgccg atctccgcgc gcacgacgat ccggtaggcc
     8401 ttgtacttgg gggtcacttg tatgggatgg gtctccatga ggtgctcccg gataataggt
     8461 tctgtaagga tccgcgggca tcacctccac cgcatctcac ccgggtgaat tacacaaccc
     8521 gcaccgagag cggataaagt cctctacagg aggacgacct tctggggccg gcttcggccg
     8581 atggctctag atcagtgccc tctggcgggc ccgttcgagc gcctcatcgc gcgaggagac
     8641 ctcgagcttg cggaagatcg atctggcatg ggtcttgacc gtattgacgg agacgtagag
     8701 ggcctgacct atctgccggt aagaatcacc gccgtcgaag agacgcagca cgtcgagctc
     8761 gcgttcggta agtacttcgg tgagcgccga ctctactcgc ctgctcttgc tcagctcatg
     8821 ctcctggcgc tcgagcagat ccgggaatat tccggcgtcg gggtaagcct ccacgatgtc
     8881 ccgcgcctcc tcgagaagca ccagggcccc tggccgatcg ccgcgcgctg ccctgacccg
     8941 cgcgagcgcc agcaacccga ggagcgtggg ccacgggttc acgtcgggcg accggcgcgc
     9001 cgcaaggccg ctttccagct cggtctgggc ctcgtcgagt ctcccgagct cgccgaacac
     9061 ccggccgagc gctatgctca ccgcgctggt ctcacggctc ccttggggaa tgaatctctc
     9121 caccagcgag cgagcttcgc gcgcgtgaga ctcggcctca tccaggcgtc cctcgtccac
     9181 ggcgaccacc gaaagtaagt acaacgcacc gatccgccac aggggttgat cggccgatat
     9241 catcgctatg gcgtcatcca gcgacttcct cgccgtcgag atgtctcccg acaggtacgc
     9301 actttgcccc agcgccatct ttaccagggc gagcctccac ggggggtctt gttcggactc
     9361 tagctccacc gagcgagcag cggccgcgac catgctcttg acgccaccat atccaaagag
     9421 ggccctgatg atggccgtct cggactcgac cgcggtgcta ccgtcgggaa gtcgtccctc
     9481 gtagggtagg ctctcggcca accgcagaaa gtgctcgctc tcctctcgcc ggccgtagat
     9541 ggcacatatc cacgctctca cgaggacgag cggcgcgtca cggcccctca gatactcggg
     9601 taacgcctcc agccagcgtt ccaggctcga aagctgcccg gtgacggcgt accgaaacca
     9661 gtgacgcgcg atcagcatac ccgccctctg gtgctctgaa gccgccatag cgtggcgtat
     9721 ggcgtcctcg aacatgccct tctcctcgaa ccaggcgccg gcccgtccgt gtaactccgg
     9781 caggaggtcc ggacggctgc tcttcagctc gtagagcagc aagtcggaga acaggtgatg
     9841 gtaacgatac cacccaccgt tctcgtcgag agcaacgacg aacaggttgg agcgggccag
     9901 ctcatccagg agcgctccgg aactctcccc gccgaccacc tcatcacaaa gcggacccgc
     9961 catctcggcc agcaccgagg tcttgagcat aaactcccgc tccgccgccg ggaggccggc
    10021 cagaacctct tctcccaaga gatcgaggat gtaacgattg ctgcctccga acgcttggat
    10081 gaaatcgtgt acgttctctt tgccctccat ggagagcgcg gccagataga tccctgcggg
    10141 ccacccctct gtgcgaacga gcagcacacg gaggtcgtcg gtgccgacct ctagatgcag
    10201 cctgccgttc agcagaaaag cagcctctct ctccgtgaaa gcgagttcct cggtgcgtat
    10261 ctcggccaat tctcccctgg ccctcagccg tcccagacgc aatggcgggt cggagcgggt
    10321 tgcgaggacg aggtggaagt tttcagggag gtgctccacg aaaaactcca tcgactcgcg
    10381 gcacccatcg tcggttgcga actggtagtc gtcgagcacc aacgttacct tctgcggcac
    10441 ctcgcctagc tcgttcacga gcctgggaag tacggtgtcg acgacccccg cgccggcgac
    10501 gttcaacgcc gcgagcgcgg aggcgccgaa cgcttttacc ggcacacccc ggcccaacga
    10561 ttcgacgata tgcctccaca gccgaatcgg atcgttgtcc tgctcgtcca gggagaccca
    10621 ggcgaaagcg ccgttagtct cgtccgacat cagccactgg gacaggagga tcgtcttgcc
    10681 atagccggcc ggagcgctga cgagagtaac cttacgctcg agccccgcct cgagcatctc
    10741 gaatagcctg gggcggatca cctgctcggg gcgcggcgaa ggtacgcgca gcttggtccc
    10801 gatgacggca gaggagtcag gcgacacgga atcgctcatc tcgggcataa tgttacaatc
    10861 tagcccaggt tttttctacc cgcaacgcga tccgcaaggt cctgtcccgc tttctgcccg
    10921 tcattcccac gcaagcaaga attcacgccc ttcgatgagg ctcggggagc cccagtaggc
    10981 gcaacggcac accgctctct cctttcgtcg cacgggaggc cattacatca tggttcttgg
    11041 ctacccacgt gcaatccggt tctggaagat cacctgggtg aacctggcgc cgggtgatgc
    11101 gagttcgccc tacccgggcg tacgatgttt gcgcaacacg agacgtctcg ggagaaagga
    11161 agaagctcat ggcagatgtc gcacaaccgc aaccgatgct ctccgacgca gccggccgcg
    11221 tctggtggac gctggtcttg aggggcgtgg tcgccgtcct cttcgggctg gcggcgctgt
    11281 tctggccggg caagaccctc ttcgtgctga ttctcttctt cggggcttac accctggtcg
    11341 atggaatctt cgcgatcgtc ggcgggataa tggactcatc ccgcaggtgg ttgctgctca
    11401 tcgagggcgt cctcggcgtg gtggcgggtc tgatcctgct cgcctggccc ggcctcggcg
    11461 cgctcgtgtt gctctacgtt atcgcgatct gggccgtcgt aaccggggtg atggagataa
    11521 tagctgccat ctcgctcagg cgggagatcg acaacgagtg gctcatgata ctcgggggcg
    11581 cgatctcggt gctcttcggg gtgatcctgg cggtcttgcc cggggcgggg ctcctctccc
    11641 tgacctggct gatcggcgcc ttcgcgctcg ttttcggcat cgccttcatc gttctcggct
    11701 tcaaggtcag gaggctagac tcgagcaggg tcagctaggg aagaactgcc gaaaggcgta
    11761 tcgaagcctc gctgtgccct tcgtcctcat ctcggttccc gcgcaggtag caaactctgc
    11821 cctggcgtag aattcccggc aagaacacct ggcgtagatt gggcgagatg accgtgaaca
    11881 tacctacccc acttgccctt atggaggcgc ttgtgggaga gaaggtaacg acatgaggcg
    11941 cgtggcgata gcgtgccagg ggggcg
//
LOCUS       DATMVF010000177         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_350107, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000177 DATMVF010000000
VERSION     DATMVF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350107"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VN178_08775"
     CDS             complement(<1..512)
                     /locus_tag="VN178_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWS81104.1"
                     /translation="MATKTLYVCSNCGHAEPKWLGRCPDCGEWSTFVEEVRESKKVVG
                     FAERATRQKARAAGRTLALSEVPVVEGGGRMDAGVGELNRVLGGGIVPGSMVLVGGEP
                     GVGKSTLLLQVMGHLGEGCLMVSGEESPRQVALSARRLGVEGRGFRVLSETDVDVIEA
                     TILQERPRAVV"
     gene            627..860
                     /locus_tag="VN178_08780"
     CDS             627..860
                     /locus_tag="VN178_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWS81105.1"
                     /translation="MQTRRTLRKVGGSVMLPIPPEMLEEMRLGAGQDVMLSSEGDVIR
                     VEPSAPRPSPDAVEFMARFTRKYDEAMRNLARR"
     gene            879..1274
                     /locus_tag="VN178_08785"
     CDS             879..1274
                     /locus_tag="VN178_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HWS81106.1"
                     /translation="MPPSGKKPGLPEILFFHTVSIEKYGGSGGVRDEEGLLAAIERPW
                     QASFGREHFSSPFEKAAALMESIIRRHPLVDGNKRTATASASYLLSTFGLEVEAEQQE
                     LEDFAVSVAVGEMKTKNTAFWFENNSLET"
     gene            1519..2727
                     /locus_tag="VN178_08790"
     CDS             1519..2727
                     /locus_tag="VN178_08790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWS81107.1"
                     /translation="MKIRSWRWVALVAAAISVLGASVGLAQKAPSPQPQAERDPAPGQ
                     VTPLAVDTFDGRVEPVDGTDDQRHLAYELRLTNITAADLTIERVRVLDPSRGGRMVDE
                     LSGDKLASRIVVFGGAPDKRFGPGVSGFLFLDVTYATGARLPERLEHRFDVSASKPTG
                     LTNSFRAGPTRVSGEEPAQIGSPLFGKRWLTGEGCCDALTSHRAAIFPIDGTFHAPER
                     FAIDLVQVGKNGRLYDGPKDKLSSYPYYGAKIRSVADGRVVGTRNDQPEQTPGLFPSG
                     LALNDFGGNYVVVKIADGRYAYYAHLQKGSKGVLVRPGDRVRKGQVIGKLGNTGNTDG
                     PHLHFMLIDGKEPLTSGALPFEIDSFRTQGVLTNYDAFLGGANAKISPEQKGEQRDRL
                     PLHRTVSDFR"
     gene            complement(2991..>3535)
                     /locus_tag="VN178_08795"
     CDS             complement(2991..>3535)
                     /locus_tag="VN178_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81108.1"
                     /translation="DPVFALFVGLLGAYLLAWRTLPNERVGEAMNEGFDATGQILLIT
                     GLGGSLGAVITQTGLQDILGGFFSAEAGTPVLLTILLAWLIAAVLHLAIGSISVAAIT
                     ASGILAPILDGLEVPAVIMALAIGSGALFALQVNSNFFWMFQSLLEVTTQGALKALTL
                     VTALASVVSLILILLLSLVV"
BASE COUNT          683 a         1138 c         1112 g          602 t
ORIGIN      
        1 accacggccc tcggccgctc ctggagaatg gtcgcctcta taacatccac gtccgtctcg
       61 gacaagaccc tgaagccccg gccttcgacc ccgagccggc gggcgctcag tgccacctgc
      121 cggggcgatt cctcaccgga gaccataagg cagccttccc ccaagtgtcc catgacctgg
      181 agcaaaagcg tgctcttgcc gacgccgggt tctcctccta caaggaccat cgagccaggg
      241 acgatcccac cccctaggac cctgttcagc tcgcctaccc cggcgtccat cctgccccca
      301 ccctccacga cgggcacctc gctgagggcc agcgtcctgc ctgcggccct tgccttctgc
      361 ctcgtcgccc gctccgcgaa accgacgacc ttcttgctct cgcgtacctc ctccacgaag
      421 gtgctccact ccccgcaatc ggggcaacgc ccgagccact tcggttcggc gtgcccgcag
      481 ttggaacaaa cataaagagt cttcgtagcc acgttccgat tgtactggat cggcgaaaaa
      541 gtacagcaaa caagaggtca ctaccgtcta aacggacaga gagttatact tcgttatatc
      601 aggttagaga cgggaggttt ggagcaatgc agacaaggcg gacattgaga aaagttggcg
      661 gttctgtaat gcttccgatc ccccctgaga tgttggaaga gatgagactc ggggccgggc
      721 aggatgtaat gttatcttcc gagggagacg ttatcagggt agagccgtcg gcacccaggc
      781 cctcaccgga cgccgtggag ttcatggccc ggtttacgag gaagtacgac gaggccatga
      841 ggaacctggc caggcggtag gctccgcgtc cgatctagtt gcctccttcc ggcaagaagc
      901 ccggtcttcc cgagattctc ttcttccaca cggtctctat agagaagtac ggaggctccg
      961 gaggcgtacg tgacgaagag ggcctcctcg ccgctatcga acgcccctgg caagcatcgt
     1021 tcggccggga acacttctct tctcccttcg agaaagccgc tgcgctgatg gagtccatca
     1081 tccgtcgcca tccgcttgtg gatggcaaca agcgcactgc gacggcctca gcttcctacc
     1141 tgttatctac gtttggtctc gaagtagaag cagagcagca agaactggag gacttcgcag
     1201 tgagcgtagc cgtgggagag atgaagacga agaacaccgc cttctggttc gagaataatt
     1261 cgcttgaaac ctgagctgca gagacgaaaa gggcggtgac caagaaagga cttacctgct
     1321 cctacttgga cctgtggcct caagcgccgc gctcgcctcc tcccggcgtc ttcgattctt
     1381 tgcgtcgggc ggagggcaag gccgtgtgcg cagccctggc tgggtgatgc gccgccggca
     1441 ccacgtagtt attgttgcat cgtcagacgt aaggacgcac catgatggcg tccggtcgag
     1501 taagcgagga gagtaccgat gaagatcagg tcgtggcgat gggtcgcgtt ggtggcggcg
     1561 gcgatatcgg tgctcggggc aagcgtgggg ttagcccaaa aggcgccgtc gccgcaacct
     1621 caggcggagc gcgatccggc gccgggtcag gttacgccgc tcgcggtcga caccttcgac
     1681 gggagggtcg agccggtgga cggtaccgac gaccagagac acctggccta cgagctcagg
     1741 cttaccaaca tcacggccgc cgaccttacg atcgagcgcg tgcgggtgct cgacccgagc
     1801 cgcggcggcc gcatggtcga cgaactctcg ggggacaagc tcgcctcgag gatcgtcgtc
     1861 ttcggcggcg cccccgacaa gcggttcggg cccggcgtgt ccgggttcct gttcttggac
     1921 gtcacctacg cgaccggcgc gaggctgccg gagcgtctgg agcaccgctt cgacgtgtcg
     1981 gccagcaagc ccacgggctt gaccaacagc ttccgagccg gcccgacgcg cgtgtctggg
     2041 gaagagccgg ctcagatcgg ctcaccgctc ttcgggaaga ggtggctgac gggcgaggga
     2101 tgctgcgacg cgctcacctc ccaccgcgcg gcaatcttcc ccatcgacgg gaccttccac
     2161 gcgccagaac ggttcgccat cgacttggtg caggtcggta agaacggcag gctctacgac
     2221 gggccgaaag acaagctctc cagctacccc tactacgggg cgaagatacg ctcggtggcc
     2281 gacggacggg tagtcgggac gcgcaacgat cagcccgagc agacgcccgg cctgttcccg
     2341 agcggcctcg ccctgaacga cttcggcggc aactacgtgg tcgtcaagat cgccgatggg
     2401 cgctacgcct actacgccca cctgcagaag ggctcgaagg gtgtcttggt ccggcccggc
     2461 gatcgggttc ggaaggggca ggtcatcggc aagcttggca acaccggcaa caccgacggc
     2521 ccccacctgc acttcatgct catcgacggg aaggagccgc ttacctcggg cgctttgccc
     2581 ttcgagatcg actcgttccg tacccagggc gtcctcacca actacgacgc cttcctgggc
     2641 ggcgcgaacg cgaagatctc ccccgagcag aagggcgagc agcgcgaccg gttgccgctg
     2701 caccgtaccg tcagtgactt ccgctgaggc ggacgtggcc agaaccccgg gataggcggc
     2761 tggcgagcac cgcaacgtgc tcgccagcca cccgctggtc tccgacttcc ccatcgcctt
     2821 cgcctgggcg ctgccgttgt cggtgaaacg gacctcggcc atcggtctgt gcggcccgcg
     2881 ggccacacac gcgacggcga gatttgacgg ctagaggtgc agagtacttg ccgccactga
     2941 ttcccgcttg accgcaaatc gcgagagtat ggcgctccag tgctgggcgg ttagaccacg
     3001 agactcagca gcaggatcaa gattagggag actaccgatg ccagcgcggt taccagggta
     3061 agggctttga gggctccctg ggtggtgacc tccagcagcg actggaacat ccagaagaag
     3121 ttgctgttta cctgcaaggc gaacagcgcc ccggagccga tggccagggc catgatcacc
     3181 gcggggactt cgaggccatc caatatgggg gccagtatgc ccgatgcggt gatggcggcg
     3241 accgagatcg aacctatggc gaggtgtaag acggcggcga tcaaccaggc gagcaggata
     3301 gtcagcagga ccggcgtgcc cgcctctgcc gagaagaatc ccccgaggat atcctggagg
     3361 ccggtctggg tgatcaccgc tcccaaagag cctccgaggc ccgtaatcag caggatctgg
     3421 cccgtcgcgt cgaagccttc gttcatcgct tccccgactc gctcgttggg cagcgtacgc
     3481 cacgccagca ggtaggcacc gagcaagcct acgaagaggg cgaagacggg atccc
//
LOCUS       DATMVF010000178         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_615396, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000178 DATMVF010000000
VERSION     DATMVF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615396"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VN178_08800"
     CDS             <1..639
                     /locus_tag="VN178_08800"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HWS81109.1"
                     /translation="IFSRALSGTANAEDGVRFTAHVLMEMARMSAEDGLVMQLHAGSL
                     RNHNETVHRRFGPDAGADIPVAADWTRGLRALLEEWGNDPRFRLVVFGLDEAAYSREL
                     APLAGHYPAMLLGAPWWFYDSPLGMRRYLDAVVETAGIYNTAGFNDDTRAFASIPSRH
                     DVWRRVTCDWVAGLLTTGLVDEEDALEMVPELAYGLAKKPYRVEEEPVRSAR"
     gene            636..1460
                     /locus_tag="VN178_08805"
     CDS             636..1460
                     /locus_tag="VN178_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS81110.1"
                     /translation="MTQIGDLFDLSGRVAIVTGATGVLGGAMARGLAVAGARVGVLGR
                     REERTKNVAEEIVDYGAEALALPADVLEREQLEGVREVTLERWGRVDILVNAAGGNVA
                     EATVADDAEVFGVPEDALRRVIDLNLMGTLLPCQVLGEAMARSGEGSIVNVSSMAAQW
                     PLTRVVGYAAARAAVENLTRWLAVELSVKHGAGLRANAISPGFFLGEQNRAMLLNEDG
                     SFTERGRKIVDHTPAGRFGEPEELVGTLIWLCSPAARFVNGIVVPVDGGFGAFSGV"
     gene            1588..2565
                     /locus_tag="VN178_08810"
     CDS             1588..2565
                     /locus_tag="VN178_08810"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWS81111.1"
                     /translation="MRPDLVLRGVIPANLMAFDADLEIDERNYRRHLRYLIDTPGVTG
                     ITTNAHASEVATLTFDEQQRSLDITLDKAAGAVHVVCGVYQDGTHKATRIAKMAERQG
                     ADCLLVFPSAVFDFGHQLRPEMAYGHIAQIAEATSLPLIVFVYPVSSGLHIATDSLLK
                     ICDEIDNVIAVKEWSNDIGVYERNWRELKALDKEISLLSSFSKSLLASLCVGADGILS
                     GHGSVIADLHVQMFDAVNRGNLEEARRVADRIYPVVQVTYAEPFLDGHNRMKEALAIL
                     GRIDEAHVRPPLRRISEAERERIRRVVTEAGLPGTRKEQPGASAVGSRG"
     gene            2597..4282
                     /locus_tag="VN178_08815"
     CDS             2597..4282
                     /locus_tag="VN178_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HWS81112.1"
                     /translation="MTIGGMEVPNRVVMPPMTTRLADPEGHVTDALVAYYKARALGGV
                     GLITVEMGSPERVGRHRFRELGVYDDRFLPGLQRLVNALHDAGAVVSVQLGHGGSRAR
                     RAVSGETPIAPSRIPTSVYETEPETVVPSEMSADRIDQTTLAFVEAARRAGRAGFDLV
                     ELHGAHGYLISQFMSPAENTRTDEYGGPLENRARFGLDILRRIKREVPSLPVVFRLGV
                     EDFFPGGLTFEEGLRVAWWTQEAGVDALSITAGHYRSLPGAERMIPPMAYPEGPFLDF
                     GARVKVAVDVPVIGVGRLGNPAIARTAIESGNVDMVALGRTLIADPNWVRKVRSGASL
                     RRCLSCNHCVNSMRSGARISCVVNPIAGHELDFADPTPPEGEEICVVGAGPAGLSYAS
                     LVADRNNVTVIERALVPGGAFRYTGKAPLFGEVEAVEGSFEAYVDGLEGACREKSVEF
                     RYGLDVTREPQVLASYDRVVFATGARYRYGLGRVVPRLLDIGLGKSRLGLRLLSSPRV
                     RDWLYYGTRRGTGPDVRQLARPDQKVTVIGDAAQAGKARDAVDGAFRAALLSG"
     gene            4292..>5146
                     /locus_tag="VN178_08820"
     CDS             4292..>5146
                     /locus_tag="VN178_08820"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HWS81113.1"
                     /translation="MNLAFREELAAVYDSACGRISRIAERLEGGREFPHVTDDGRWRT
                     WPADVYAGWEGDNWSHGNWTCGFWVGLLWLAFLRTREPRFEEWARYFGDLVAPRQHDV
                     NTHDIGFVFYPSFALGYEITGEEELKQPAVQAARTLIRRFNRRGEYLQAWGPLDHPLA
                     KGSTAIDTMMNLPLLWWAARTTLEREFADVARAHAATSARYYLRPDGSTYHIYTFDPD
                     TGAGIEGGTYQGANPDSTWSRGVTWGIYGWTLAYREHRDPAFLEAAEKAAAYFVGALP
                     ADLVPPWDF"
BASE COUNT          925 a         1593 c         1709 g          919 t
ORIGIN      
        1 atcttcagcc gtgccctctc cggaactgcg aacgctgagg acggcgtccg gttcactgcc
       61 cacgtgctta tggagatggc gcgtatgagc gccgaggacg ggctggtgat gcaactgcac
      121 gccggcagcc tccgcaacca caatgaaact gtgcaccggc ggttcgggcc cgacgccggg
      181 gctgacatcc ccgtagcagc cgactggacg cggggtctgc gggccctcct cgaggagtgg
      241 ggcaacgacc cgcgcttccg tcttgtcgtg ttcggcctcg acgaggccgc gtattccagg
      301 gagctcgcgc ccctggccgg acactacccg gcgatgctgc tcggagcgcc gtggtggttc
      361 tacgacagcc ccttagggat gcggcgctac ctggacgccg tggtagagac cgccggcatc
      421 tataacaccg ccggcttcaa cgacgatacc cgcgcattcg cctcgattcc ttctcgccac
      481 gatgtctggc gacgcgttac ctgcgactgg gtggccgggc tcctcaccac tggcctcgtg
      541 gacgaggagg acgccctcga gatggtcccc gagctcgcct acggtctggc caaaaagcct
      601 taccgggtcg aagaggagcc tgtgaggtct gcccgatgac gcagatcgga gacttgttcg
      661 acctctccgg gcgggttgct atcgtaaccg gggcgacggg cgtgcttggc ggtgcgatgg
      721 ccagagggct ggccgtagcc ggggctaggg tcggcgtcct gggacgtcgc gaggagcgca
      781 ccaagaatgt cgccgaagag atagtggatt acggcgccga ggcgctggct ctgcctgcgg
      841 acgtgctcga acgcgagcaa ctcgaaggcg ttcgggaggt tactctggag cggtggggcc
      901 gggtggatat cctggtaaac gccgccgggg gtaacgtggc ggaagccacg gttgcagacg
      961 acgccgaagt gttcggtgtt cctgaggacg ccctgcgacg ggtcatagac ctgaacctga
     1021 tgggtaccct gctgccctgc caggtcctgg gcgaggcgat ggcccgatca ggtgagggct
     1081 caatcgtcaa cgtctcctcg atggcggcgc agtggccgct cacgcgcgtc gttggctacg
     1141 cggcagccag ggctgcggtc gagaacctaa cacgctggct cgcggtcgag ctttcggtca
     1201 aacacggagc gggcttgagg gcaaatgcca tctcgcctgg gttctttctt ggtgagcaga
     1261 accgggcgat gctgctcaac gaagacggaa gcttcacgga gaggggacgt aagatcgtgg
     1321 accatacccc cgctgggcgc ttcggcgaac cggaggagct ggtggggacc ctgatctggc
     1381 tttgctctcc cgcggcgagg ttcgtgaacg gcatcgtcgt tcccgttgac ggcggcttcg
     1441 gcgccttcag cggggtatag aaagatactt gatgcgtctc gaagctaccg aggacgccat
     1501 acagaaagta ttgagcacta tgaaaggcca ccatcgaggt ggtaaggtaa ctggtatggc
     1561 aagcgaggag tgagggaggt ctgctccatg aggccagatc tggttctcag gggtgtcata
     1621 cccgccaacc taatggcgtt cgatgccgac ctggagatcg acgagagaaa ctaccgcagg
     1681 cacctacgct acctgatcga cacgccgggg gtaaccggca tcactaccaa cgcccacgcc
     1741 tccgaggtag cgacgctgac cttcgacgaa cagcagcgca gcctcgacat aactctggat
     1801 aaagcggcag gggcggtaca cgttgtgtgc ggcgtctatc aggatggcac ccacaaggca
     1861 acccggatag caaagatggc cgagcggcag ggagcggact gtcttctggt attcccctcc
     1921 gcggtcttcg atttcggtca ccagcttcgt cccgagatgg cgtacgggca catcgcccag
     1981 atcgccgaag ccaccagcct cccgctcatc gtgtttgttt atcctgttag ctcaggtctg
     2041 cacatcgcta cggacagcct actaaagatc tgcgacgaga tagataacgt catcgccgtc
     2101 aaagagtggt ccaacgatat cggcgtctac gagcgcaact ggcgcgagct taaggcgctg
     2161 gacaaggaga tatcgctgct ctccagcttc agcaagtcgc ttctagcgtc cttgtgcgtc
     2221 ggcgctgacg gcatcctctc ggggcacggc agcgtcattg ccgatctgca cgtgcagatg
     2281 tttgacgctg tgaaccgcgg caacctggag gaggcgcgca gggtggcgga ccggatctac
     2341 cccgtggtgc aggtcaccta cgccgagccg ttcctcgacg ggcacaacag gatgaaggag
     2401 gccctggcca tcttaggcag gatcgacgag gcccacgtcc gtcccccgct gcgccgcatc
     2461 tcggaggcgg agagggagag gatccggcgc gttgttaccg aggccggatt gccgggcaca
     2521 cgtaaggagc agccgggcgc tagcgccgtt gggagccgcg gctagggcaa gtgccgtgaa
     2581 cgttctcttt tcccccgtga ctatcggagg gatggaagtt cccaatcggg tggtcatgcc
     2641 gcccatgacc acccggctgg cggacccaga gggccacgtt acggacgccc tcgtcgccta
     2701 ctacaaggcc cgggcactcg gcggcgtggg cctgatcacg gtcgagatgg gctctccaga
     2761 gagggtcgga cgccaccgct tccgcgagtt gggcgtctat gacgaccggt tcctacccgg
     2821 gctccaacgc ctcgtcaacg ccctgcatga cgcaggagcc gtggtctctg tccagctcgg
     2881 gcatggcggg agccgggccc gcagggccgt ctccggcgag acacccatcg ccccttctcg
     2941 gatccccacc tccgtctacg agacagagcc cgagacggtt gtgccctcgg agatgagcgc
     3001 ggatcgtatc gatcaaacga cgctggcctt tgtcgaggct gcccgacgcg ctgggcgcgc
     3061 cgggttcgat ctcgtcgagt tgcatggagc acatgggtac ctgatctccc aatttatgtc
     3121 gcctgcggag aacacccgga ccgacgagta cggcggcccg ttggagaacc gcgcgcgctt
     3181 cggcctagac atcctgcgac gcatcaagcg cgaggtgcct tctcttcccg tagtcttcag
     3241 gttaggtgtt gaggacttct tccccggggg gctgaccttc gaggaaggac tccgggtcgc
     3301 gtggtggacc caggaagcgg gcgtggacgc cttgagcatc accgccggac actatcgctc
     3361 gcttcctggg gctgagcgca tgatcccgcc gatggcctac cccgagggcc cgttcctcga
     3421 ctttggcgct cgggtcaaag tggcggtcga tgtcccggtc ataggggtgg gccgcctggg
     3481 aaaccccgct attgccagga ccgccatcga gagtggcaac gtggacatgg tcgcgcttgg
     3541 gcgcacattg atcgccgatc cgaactgggt taggaaagta cgctccggag cgtcgttgcg
     3601 ccggtgccta tcctgcaatc actgcgtcaa cagcatgaga tccggcgccc gcatctcctg
     3661 cgtggtaaac cctatcgccg gtcacgaact cgacttcgcc gacccgacgc ctcccgaagg
     3721 agaggagatc tgcgtcgtgg gtgccggccc cgcagggctc tcctacgcat ccctggtagc
     3781 cgaccgcaac aacgtcacgg tgatcgaacg agcactagtg ccaggggggg ccttccgcta
     3841 tacgggcaag gccccactat tcggcgaggt cgaagcggta gagggatcgt tcgaggctta
     3901 cgtggacggg ctcgaagggg cgtgccggga aaagagcgta gagttccgct acgggctcga
     3961 tgtcaccaga gaaccacagg tcctagcatc ttacgatcgc gtagtgttcg ctaccggggc
     4021 gcgctaccgg tacgggctcg gccgcgtggt accccgcctc cttgacatag gtctgggcaa
     4081 gtcgaggttg ggcctgcgtc tgctctcttc gccgcgggtg cgggactggc tctactatgg
     4141 aactcggcgc ggcacgggac cagacgtacg gcaactcgcg cgtcccgacc agaaagtaac
     4201 ggtcatcggc gatgctgctc aggccggcaa ggcgagggac gccgtcgatg gagcgttccg
     4261 cgcggcgcta ctgagcgggt aggaggaagc attgaaccta gcgtttcggg aggagctcgc
     4321 agcagtctac gattcggcgt gcggccgcat ctcacgcatc gccgagcgcc tggaaggggg
     4381 ccgggaattt ccccacgtga cagatgacgg ccgctggcgc acgtggcctg cagacgtgta
     4441 cgctggatgg gaaggcgaca actggagcca cggaaactgg acatgcgggt tctgggttgg
     4501 cctcctgtgg ttagccttct tgcgcacccg ggagccccgt ttcgaggagt gggcacggta
     4561 tttcggcgac ctcgtagccc cccgacagca cgacgtcaac acccacgaca tcggctttgt
     4621 cttctatccg agcttcgccc tcggttacga aatcaccggt gaggaggagt tgaaacagcc
     4681 cgccgtgcag gcagcgcgaa ccctgatcag gcgcttcaac cggcgaggag agtacctcca
     4741 ggcctggggg ccccttgatc accccttggc caagggatca acggccatcg ataccatgat
     4801 gaacctgccg ttgctctggt gggccgcgcg gacgaccttg gaacgagagt tcgccgacgt
     4861 agcccgcgca cacgcagcga cttcggcccg ctactatcta cggcccgacg ggtctacgta
     4921 tcatatatat accttcgatc ccgacacggg cgccggtatc gaaggcggca cctatcaggg
     4981 tgccaaccca gactctacgt ggtcgcgcgg ggtaacgtgg ggaatctacg gctggacttt
     5041 ggcttaccgg gagcatcgcg accccgcttt cctggaagcg gccgagaagg ccgcggctta
     5101 cttcgtggga gccctgcccg cggacttggt cccaccctgg gacttc
//
LOCUS       DATMVF010000179        26119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_165766, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000179 DATMVF010000000
VERSION     DATMVF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 26119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26119
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165766"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VN178_08825"
     CDS             complement(<1..433)
                     /locus_tag="VN178_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HWS81114.1"
                     /translation="MRPRNVKEMLIEAKNTSELMVDLSYAAIFYNSEDLSEEVSHLEE
                     RLNDLVFDMRTLTILAARSPADAEQMAGILQVVQDIEKIGNAAVDIAKIVVKRLGIPP
                     ELLHDIPEAEEIPSRVRVHPDSALDGRSLGDVDLPVEMGMSP"
     gene            complement(446..1696)
                     /locus_tag="VN178_08830"
     CDS             complement(446..1696)
                     /locus_tag="VN178_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWS81115.1"
                     /translation="MRDRLRNRPRLRPKVSGYLTEEKESLRQGFAALFLSSAGELIAG
                     IVLAGISGTLDELVGLAVLIPAAIGMRGAIFGAMGSRLSTSIQTGLFELNLRRGVLAE
                     NVQAAAVLSLVSGVFLAFLARVLCGILGVRTELSVIDYVIISTVGGIIAGVLLLGITV
                     FVARLSVARGWDMDNIAAPMLTAAGDILTLPSLVVATYLIGIPIFSSALGIALVMSAV
                     AAAFFGLRIGVENLRRILAESFPILAMTGTVTILVGVALQDREEQFTTLLALSILLPA
                     FLQEGGALGGILSSRLSSKIHLGLIETRGVPQFAALRDFTLTYIFAAGVYVFIGGASH
                     LLAVALFQEGASPGFLMMIGISALAGLIATTAAVFAAYYGSTLSYRLGLDPDTYGIPI
                     ITAAVDLLGFMSLIIALIVFGLAG"
     gene            1923..2873
                     /locus_tag="VN178_08835"
     CDS             1923..2873
                     /locus_tag="VN178_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWS81116.1"
                     /translation="MSGIRAVVVDPDAPNRLALADVEEPSPTPSEVLVRVSAVSLNRG
                     EVRRAESSEPGFRPGWDLAGTVERAAADGSGPREGSRVVGFLPSGAWAELAAVPTDSV
                     AVVADGVSFEEASTLPVAGLTALYALEEGGNLLGRTVLITGASGGAGQFALQLARAAG
                     ARVVGLVRREEHVSLVEEAGAHEVVVDESGEAAGEKGPYHLILESVGGEVLGNALSML
                     APGGTCVSFGTSATADVEFDARTFYLTGGARLYGFILFHEVLARPASQGLHRLARMVA
                     DGMLKPRIEVEAPWTELGEIAARLLERGYTGKAVLRVSGA"
     gene            complement(2950..3378)
                     /locus_tag="VN178_08840"
     CDS             complement(2950..3378)
                     /locus_tag="VN178_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81117.1"
                     /translation="MGTFNSTKTVPYVVEDLGPVAQDLVRYFEGQDFETTETNLPAGG
                     VQVSIRKGGTFKAIIGMKTALNIKVEPVEGGTQVDAGVGIFGQQAIPTIITALVFWPV
                     IISQVWNMAQEAKLDEEAIGVAEESLKAHASGAPSPLAQT"
     gene            complement(3571..3984)
                     /locus_tag="VN178_08845"
     CDS             complement(3571..3984)
                     /locus_tag="VN178_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWS81118.1"
                     /translation="MDVRAAVFQDGRILLVKESGEPGWSLPGGWADVGESPSEAAARE
                     TLEESGYRVRPVRLLAAYDRDRHGHPPIPYHVYKLVYLCEILDEMPLSDVDTDGVRFF
                     GEDELPELSVTRVTPAQVSRFFEHHLDPNLLPDFD"
     gene            complement(4252..5232)
                     /locus_tag="VN178_08850"
     CDS             complement(4252..5232)
                     /locus_tag="VN178_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HWS81119.1"
                     /translation="MELGGLHHMTAVTTNASENVAFYTDVLGLRLVKKTVNQDDVSAY
                     HLFYGDEVGNPGTEITFFDWPHLRVAPNHPGAGEVSTTELRVPDRAALDWWAARFRDL
                     GVDNNGIEETEEHSYLDFTDPEGQRLRLSADEGEGVQGGDPWEESPVPREMGIRGLGA
                     VDLTVRELEPTAWVLTEVLGFRRVSAVVQNGGQTVTFEVGGGGPGATVRVIERPDAPW
                     THLGRGGVHHIAFRTPDDEEHAAWREKIRRSGLGVTPQIDRFYFRSIYFREPGGVLFE
                     IATDGPGFATDEDAAHLGERLSLPPFLEERREEIEAGLDPIIVEGGTELR"
     gene            5794..6822
                     /locus_tag="VN178_08855"
     CDS             5794..6822
                     /locus_tag="VN178_08855"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWS81120.1"
                     /translation="MRSGTHTFLQTTRVHFGADALEKLEEEAWSYDRAFVITGRSLNE
                     KTNLVRRVEALLGGRHAGTFAGIGQHTPGGAVEQAAVGAEMTAADLLVSVGGGSVIDG
                     TKAVARELGYPQQVAIPTTLSGAEWANRAGVTDERSGRKRGFADPKTVPRVVVLDPEA
                     TVFTPEKLWLSTGIRALDHAVEGYLYGGDHPITDVTGLEGARRLMEYLPLSRHEPDDL
                     DVRLELQLASWLAYFGPANTPMGLSHGLGRRIGASYEVPHGITSCITLAPSLRLAKKR
                     VQDVRWSKLSEALGGEPSERVSSLVQELGLPGRLREVGVPEEDLESIAGEYGDKAGEA
                     LAILTAAY"
     gene            6835..7812
                     /locus_tag="VN178_08860"
     CDS             6835..7812
                     /locus_tag="VN178_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003772115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HWS81121.1"
                     /translation="MIPARLERGDEVRVVSPAVSLGFIPEEQKLIARERLEDFGLRLS
                     FSTNVGVMDRFDSSPVQARVSDLHEAFSDPEVKGILTTLGGYNSNGLLGYLDYGLFRA
                     NLKIFCGFSDITALATAIHARTGLVTYSGPHFTTFGMKRGIEYTEEYFERCLMRGQPF
                     EVLPADHWSDDLWYQDQEDREFVPNPGYEVMYEGEAEGKLLGGNLGTLCLLFGTPYMP
                     DLEGSILLLEDDEEVNAVHFDRALQSLIHQPGFEGVQGMVFGKFQRASNMDQDTLTAI
                     VESKRELDRMPIVAGASFGHTTPQFTFPIGGYGRLHAGDGAARLSINVH"
     gene            complement(7864..9495)
                     /locus_tag="VN178_08865"
     CDS             complement(7864..9495)
                     /locus_tag="VN178_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HWS81122.1"
                     /translation="MHQIEIILGLLLAVAALATLATRLKIPYPILLVIGGSALGFVPG
                     LPQVALDPELVFLLFLPPLLYVSAIFTSWRDFRGNIRAISLLAVGLVLMTTFVVGVVV
                     HTVSGIPWAAAFVLGAIVSPTDAIAATAVAQRLGVPRRIVTILEGESLVNDATGIVAY
                     RVAVAAVVTGAFSIWEAGLQFVVGAAGGIAAGFAVGWLVIWARRHLSEDPSVQNIVSL
                     LTPFVAYLAAEELPHSVWEMLHELFGVPGELYFSGVLAVVTTGLYLGRKGPYIISSGT
                     RLQGYATWELITFLLNGLIFILIGLQLRSVVGGLGEFTAGEIISYAVLVSVTVILVRI
                     IWVFPATYVPRWAFRRIRERDPSPPWRSVSVVAWTGMRGVISLAAALALPLQTAEGVA
                     FPARDLIIFLTFCVILATLVVQGLSLPVLIRALGLEDDHIGDKEETHGRIQVAEAALQ
                     RLEELVEEDWVREDTTERVRGLYTYRRNRFATRFDGDPEGVEERSAAYQRLMVELLAA
                     QRLRLVTLRDEGSIGDEIMHRIERDLDLEESRLEL"
     gene            complement(9515..9814)
                     /locus_tag="VN178_08870"
     CDS             complement(9515..9814)
                     /locus_tag="VN178_08870"
                     /inference="COORDINATES: protein motif:HMM:NF023070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81123.1"
                     /translation="MIVLVDLKEDVVPEEYERWLQERYVPAILDLASVDEWRGYRVGG
                     LPESGGDPPYQYVVSVGINDLEQLGREMESERVQRLLSELGRYAYVTQLMTERFV"
     gene            complement(9852..10130)
                     /locus_tag="VN178_08875"
     CDS             complement(9852..10130)
                     /locus_tag="VN178_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HWS81124.1"
                     /translation="MRRLMQMFQQQCDHIDQIRDVEPSAQGCEECLKTGDAWVHLRKC
                     LVCGHVGCCDQSPNRHATAHFHATGHPIITSFEPGEDWRWCYVDEVLV"
     gene            complement(10142..11764)
                     /locus_tag="VN178_08880"
     CDS             complement(10142..11764)
                     /locus_tag="VN178_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81125.1"
                     /translation="MSNIGNPPCDVGVIRSHRADAPCEQTSRPWVLAATIIGSAMAFI
                     DSTVTNVALPQIQERLGATATDAQWIVESYALFLSALILVGGSLGDHFGRRRIFSLGV
                     IVFTLASVWCGLSTSPEMLIVARAAQGVGGAMLVPGSLAIISASFDEEDRGKAIGTWS
                     GLSGVTTALGPVLGGFLVENVSWRAAFLINVPLAVAVLLITARYVPESRDPDARELDI
                     PGAAFATIGLGGVVYGLISSAEAGFTDLRVILPLVLGVGALVAFVVRERRTEEPMMPL
                     SLFRSRNFSGANILTPLLYAGLGGALYFLPFLLIQVHGYSATAAGSSFLPFTIITFVM
                     SRWAGGLVIRFGARLPLMIGPVIAAAGFVLFALPGIGGSYWTTFFPAIVVLGLGMALV
                     IAPLTTTAMNSVSGSHSGLASGVNNAVSRTAGLLAIPVLGIFVFLVFSGGLDARVQNL
                     DLSPQAQEQLEAEKVNLGGAQVPAEVSEGTAVEIERAIQESFLAGFRLAMFVAAGLAV
                     ASAVAAAVIIEGKGSTAPREKAGRPEGETAAA"
     gene            complement(11796..12374)
                     /locus_tag="VN178_08885"
     CDS             complement(11796..12374)
                     /locus_tag="VN178_08885"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HWS81126.1"
                     /translation="MPSRADEVLDFWFGREGEPGYGEFRDEWFRKDPEFDATVTDRFA
                     GLYDGAVAGDLDGWRDDARSCLALVIVLDQFPRNMFRGDGRTHAADGAALESSRYAVE
                     HALDRELPAFQRMFLYMPFMHSENREDQRRSVELFGLLADEPGAPDVTSYAAAHRDIV
                     ERFGRFPHRNEILGRETTPEEAEFLKTEGSSF"
     gene            complement(12364..12951)
                     /locus_tag="VN178_08890"
     CDS             complement(12364..12951)
                     /locus_tag="VN178_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HWS81127.1"
                     /translation="MGKTQVFHEICTEKREVDRRVLEETVSLAVEIAREGREGRKIGT
                     LFVVGDSGEVTKRSKPLILDPLHGHPDESKQIEDPDMRETIKELAQLDGAFLVSNRGV
                     VLSAARYIDAASDNLDLPLGLGSRHVAGASISRQTRAVAVVVSESSMVRMFDDGELVS
                     EIVPELWMIDGYKSRMEGRIFTRRDEDVTVIGRAE"
     gene            complement(12957..14330)
                     /locus_tag="VN178_08895"
     CDS             complement(12957..14330)
                     /locus_tag="VN178_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS81128.1"
                     /translation="MMRDARDEQALTPEQIEALMSYGREFALQTGDLLFDELSVVDSF
                     YIVLEGEVEISRLDGAEEIPIGNHGAGEFTGGLVMLTGRTSIHRARAAAPGRVLEIDS
                     ETFRRLPVEVPDVADMLISGLARRMRYTQRAYRQQEKFAALGKLSAGLTHELNNPAAA
                     ARRASEELGGAILEAQLSAIGHDERFSAPEREALVALQRETAAGTADLLDPLSLGDAE
                     DELADWLEDHGCEDSWDLAPALAEAGVKKDRLEAMAEAFGNDSLACGLEWLAGTLDLV
                     GLAGEIETSAGRISELVGAMKEYTYMDRAAVGEVDVISGLKNTLTILGPRLKNVSLSQ
                     EYEKSLPRIPGRGGELNQVWTNLIDNAIDAVDGRGSITVRAYAEGARVVVEVADDGPG
                     IPRAAQVHVFEPFYTTKDIGDGTGLGLAIVRRIVTDHGGEIFVQSEPGETCFTVRLPI
                     DTRQNRG"
     gene            complement(14327..15739)
                     /locus_tag="VN178_08900"
     CDS             complement(14327..15739)
                     /locus_tag="VN178_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS81129.1"
                     /translation="MPGERVLDCLRSVPLFRRLSDEKLAWIHDHAEEVRLDAGTLIAR
                     QGDPPDGFYVVMEGETEWTRRVGQEDVFVVTLGEGTIFAELIMVLDAPYPTTGHAITD
                     VGLLKLDVPSFWEMLRICPEVLRDILATSVERAELHESVSQQHGKLISLGTMAAGLAH
                     ELNNPAAAIGRSAAEAREAFQEASEKAVELGNLPLTEEQRSFVAGLPQEASQKSGTTL
                     DSLEVSDLEDELAVWMEDHGVEEGWEISPTLAGAGLDAVWLEALAERLPDEALGGVLG
                     WVRATVAGDELLREIEGGSARISELVGAIKTYTHMDRAASKRVDVHAGLDSTLIMLGH
                     KLKKGDVEVVRDYEKDLPHVCGHAGELNQVWTNLIDNAIDAVDGRGRITVRTASENGG
                     VLVEVSDDGPGIPEEVRGRIFEPFYTTKDVGEGTGLGLDISYRVVVEDHKGDIRVLSE
                     PGDTRFQVRLPVDKSTGAHS"
     gene            complement(15741..17402)
                     /locus_tag="VN178_08905"
     CDS             complement(15741..17402)
                     /locus_tag="VN178_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS81130.1"
                     /translation="MAKPVILAVDDDPQVLRAVERDLRRRYAREYRILRADSGESALD
                     TLGKLKLRGDPVALFLVDQRMPKMTGVEFLEHAIERYPEAKRALLTAYSDTEAAIRAI
                     NEVGLDYYLQKPWDPPDENLYPNLDDMLADWRADYRPPFTGLRVVGDRWSPESHRTRE
                     FLALNRVPYRWLDIEGSEEAREIVANADSGAPKLPLVVYEDGSYAEAPQNREIADKIG
                     LQTRAGQQFYDLVIVGGGPSGLAAAVYGASEGLKTVLIEREAPGGQAGTSSRIENYLG
                     FPAGLSGGDLARRAVDQAKRFEVEILTPQEVSAVRVKDPYRIVTLGDGTEISCYALII
                     TTGVSYRKLDVPGVDRLTGRGIYYGAAQTEALSCVGEDVFIVGGANSAGQAAMYFSNR
                     ASRVFILYRGGDLRKSMSDYLVRQIEARDNIEVLLDTNVVSVEGKDHLERITAKNTVT
                     GETETTAAHSLFVFIGAAPKTGWLDGVVERDERGFILSGPDVMHEGKRPKGWRPDRDP
                     FLLETSVPGIFVAGDVRHGSVKRCASAVGEGSIAIQFVHQYLREV"
     gene            complement(17526..18182)
                     /locus_tag="VN178_08910"
     CDS             complement(17526..18182)
                     /locus_tag="VN178_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS81131.1"
                     /translation="MSADLGFVHRYVPGEDESGPTLLLLHGTGGNEEDLAPLGETLAP
                     GGALLSPRGKVSEYGAPRFFRRLAEGVFDHEDLLFRTHELAEFVQAASEEYGFDASKV
                     VAVGYSNGANVAASTILLHPGLLRAAVLFRAMVPFEPEVTPDLSGMPVFIAAGRMDRM
                     IPPDNTQRLADILLEAGADVDLRWRDAGHPLTYEDVAEAKAWLSDVLPKLSGPQNGRE
                     "
     gene            complement(18179..19126)
                     /locus_tag="VN178_08915"
     CDS             complement(18179..19126)
                     /locus_tag="VN178_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HWS81132.1"
                     /translation="MAQTTGIHHVTAISGEPQRNVDFYAGTLGLRLVKKTVNFDDPET
                     YHLYYGDGAGSPGTIMTFFPWAHAPGGRIGAGQLVVTSFSIPTTSLGYWTERLVEKGV
                     RFERPRDRFGDTVLTFEDPDRLRIELVAAEDGRPGWSGGPVQADHSVRGFHHVALAVE
                     APERTAGLMTDFLGFRRVDEAEGRIRLASGKGGPGDLVDVVNAAGFPHGSMGVGTVHH
                     VAFRVPDEDTQLAVRTEISALGYSVTPVLDRNYFRSIYFREPGGVLFEIATDPPGFTV
                     DEDPEHLGEDLKLPPWLETRRDRLEEVLPSLRVPVGENA"
     gene            complement(19301..20173)
                     /locus_tag="VN178_08920"
     CDS             complement(19301..20173)
                     /locus_tag="VN178_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS81133.1"
                     /translation="MSVLPERILLPTDGSEDAIRATEAASDLARRSGAELHVVHVWHD
                     VRGFAHDFVKRELRRQGQEILDEQVERIKASGGEVAKAYLRSGRTSNEVIALCKEIDA
                     GLLVVGSRGLGTVRRILMGSQSEEIVHHAQVPVLVLTGAQDFWPPARIVIGEDFSDDA
                     RKAGELATGIGKLYDAEGLLVYSHPDLPEVPPGETRSRVQNLVDMRERDREMLEERAG
                     ALEETLGSKPEILISGGYPANVLLEASQEVQPSLVAVGSRGLAGLIRTRLGSVSTKVV
                     TAARGPVLVYPHVD"
     gene            complement(20248..22032)
                     /locus_tag="VN178_08925"
     CDS             complement(20248..22032)
                     /locus_tag="VN178_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS81134.1"
                     /translation="MPQERAGDRGATTRRLLGELRPYSGKLMIVFGLVVLGAAAQAGG
                     PWLIGRAIDRYILRGDLAGLSRIMLVLLGVYVVGAIASRGQVYQVGSIGQSLLASMRA
                     RIFDRLQHLPLRYFDRRPVGDLMSRVTNDVDTLNQLFSQGLTQLLGSLFSLIGIVVAM
                     LFLDWNLALACFTIIPIMLVLTSFFARRARRAFRLTRETVGDVTAGVQEDIVGVREAQ
                     AFNRTEENIARFRRRNAANRDANVQAVAITSAFAPTIDMLSTLATALVIGYGGYLVFA
                     GSLSIGILTAFLIYVQQFFRPIQLASQVYTQAQAAVAGAERIYNILDEEPEPEDPHDT
                     PKLGQATGRISFEHVTFAYDPGNPVLHDVDFRVEPGMTVALVGPTGAGKTTIASLIPR
                     FYDATEGRVLIDGRDVRELARKDLRANIGIVLQEPFLFSGTIAENIGYGRSGDPSEAT
                     REDVEAAARVVNANDFIMALPEGYDTVLGEGGGALSQGQRQLLSFARAVLTDPRILIL
                     DEATSNIDTRTEAIIQKALATLLEGRTSIVIAHRLSTIRNADLILVIQDGSIAERGDH
                     ATLLGKNGLYADLYRRQFREPVPSGGRS"
     gene            complement(22102..22482)
                     /locus_tag="VN178_08930"
     CDS             complement(22102..22482)
                     /locus_tag="VN178_08930"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HWS81135.1"
                     /translation="MNDSFGEVTLRLLDETKEVRIETRRDDDAPEHRTIIWVVVVEGE
                     VFVRSVRGTKGRWYREISANPEGALHVEDDRIPVKAAPATEEATVNAVSAAFRSKYQQ
                     SSPSSTEAMVRPETLPTTLKLSPA"
     gene            complement(22491..23039)
                     /locus_tag="VN178_08935"
     CDS             complement(22491..23039)
                     /locus_tag="VN178_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWS81136.1"
                     /translation="MSATANERTAKDRISFLRTLRSVRSFRSDPIPQEVVDDVLEVAR
                     WSGSATNKQPWEILIIRERETLRSLASAGGYAGHLAEAPLGIVLVMAGERAEQETYDE
                     GKLAERIMLAAHAHGVGSSIGWFAGPGRDAAREILGVPREKVVRTAISLGYPAEEARR
                     SRGGRRQARKPLGEIVHHERYA"
     gene            complement(23107..24873)
                     /locus_tag="VN178_08940"
     CDS             complement(23107..24873)
                     /locus_tag="VN178_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS81137.1"
                     /translation="MNTPSGAGELRRALAYLRSYTWQSVVAVLSLLLVSGANLATPLL
                     IGRAIDEGIGPRRLGVIFVVVGWLVGLALARALFTFLQGYLAERASQGVAYDLRDALF
                     ERIERLSFSYYDRVQTGQLVTRLTSDVEQIRTFAGSGVVQLANAVVMLIGTTILLLYL
                     DWQLALVALAVVPIIAVLLVRFVGRIRPLFREVQQTLGRLNTVLQEDLLGVRVIRAFA
                     REDYETARYTAVNEELLEKNLTTVRVVSNNFPFVFLFANLGTLAIIWFGGWQVIGGRL
                     SIGDLVAFNTLLGFMLFPILTIGFLSASISRAGASSQRVFDVLDAPLDVEDAPDASTL
                     LPLSCRVDFDNVSFRYPGSARDILAGVSFTARPGQTVAILGTTGSGKSTLVNLIPRFY
                     DVTGGAVRLDGNDVRDVTLSSLRSQIGIVLQETRLFSGTVRDNVAFGKPDATDEEVVA
                     AAEAAQAGKFVRGLSEGYDTVIGERGIGLSGGQRQRIAIARALLIDPRLLILDDSTSA
                     VDAETEAAIQETLDRLMREKHRTVFVIAQRVSTVRDADLILVLDEGRIAARGTHEELL
                     RESELYNEILGSQLLSPSETAA"
     gene            complement(25017..>26119)
                     /locus_tag="VN178_08945"
     CDS             complement(25017..>26119)
                     /locus_tag="VN178_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HWS81138.1"
                     /translation="GDLAPLAHVGLCLVGEGRLLEGEEPVKTSEALERAGIRPLGLAT
                     KEGLALINGTDGMLAMLLLSLEDLKVLLKTADVTAAMSVEALLGTDRVFREDLHALRA
                     HPGQLASAGNISKLLSGSPIVASHRDSPHLVQDAYSLRCAPQVFGAARDTLDHASLVA
                     GRELDSVTDNPVVLPDGRVESVGNFHGEPLALVLDFLAVAASEVGAITERRIDRMLDP
                     RRSEGLPAFLSPRAGVNSGFMIPHYTAASLAEENRRLANPASTGSLPTSAMQEDHNSM
                     GWSAGRKLRRLLANVASILAVEAACAAQALDLRSPLQPGHATGAVLERIRRDIPFIES
                     DTFMAPHLEAAERLVNSGALVEAAEQEIGPLN"
BASE COUNT         4648 a         8413 c         8339 g         4719 t
ORIGIN      
        1 tggggctcat tcccatctcc acgggcaggt cgacgtcgcc caaagagcgc ccgtccaggg
       61 cgctatccgg gtgcacgcgg acgcgggagg gtatctcttc ggcctccggt atatcgtgca
      121 ggagttcggg cgggatccca agtcgcttta ccacgatctt ggctatgtcg acggcggcgt
      181 tgccgatctt ctctatgtcc tggacgacct ggaggatccc cgccatctgt tccgcgtcgg
      241 cggggctcct cgcggccagg atggtcaggg tgcgcatgtc gaagaccagg tcgttgagac
      301 gttcctcgag gtgggagacc tcctccgaga ggtcctcgga gttgtagaag atcgccgcgt
      361 aggagaggtc caccataagc tccgaggtgt tcttcgcctc gatgagcatc tccttgacgt
      421 tccgtggtcg catctcgcgc atctcttatc ccgccaatcc gaaaacgata agggctatta
      481 taaggctcat aaagccgagc aggtctacgg ccgccgtgat tatcgggatg ccgtaggtgt
      541 ccgggtccag cccgagcctg taactgaggg tcgagccgta gtaggccgcg aagaccgccg
      601 ccgtggtggc gatcaggccg gcaagggcgc tgataccgat catcatcagg aagccagggc
      661 tcgccccctc ctggaagagc gccacggcta gaagatgaga agccccaccg atgaatacgt
      721 agacgcctgc ggcgaagatg taggtgagcg tgaagtctct caaggcagcg aactgcggta
      781 ccccgcgcgt ctctataagg cccaggtgga tcttggagga gaggcggctg gagagtatgc
      841 cgccgagcgc tccaccctcc tgcaaaaagg ccggcagaag gatgctcaag gccaggagcg
      901 tagtgaactg ctcctcccta tcctgcaacg ccacgccgac caggatggtg accgtccccg
      961 tcatggccag gatggggaag gattcggcca ggatgcgcct caggttttcg acgcctatcc
     1021 tgaggccgaa gaaggcggcg gccaccgcgg acatgaccag tgcaataccg agtgccgaag
     1081 agaagatggg gatgcctata aggtaggtgg ccacgacgag agagggaagg gtgaggatat
     1141 cccccgctgc ggtgagcatg ggggccgcga tgttatccat atcccagccg cgggcgaccg
     1201 agaggcgggc gacgaagacc gtgatcccta gcagcagcac gccggcgatg atcccgccca
     1261 ccgtcgaaat gatgacgtaa tcgattaccg agagctccgt cctgacgccg aggatgccgc
     1321 acaggactcg cgccaggaaa gcgaggaaca cgcctgagac gagcgagagg acggccgcgg
     1381 cctgcacgtt ctcggcgagc acgccgcgcc tcagattcaa ctcgaagaga cccgtctgga
     1441 tggaggtcga gagcctgctt cccattgccc cgaagatggc gccgcgcata cctatggccg
     1501 cgggaatcaa gacagcgagc ccgacgagct catcgagcgt gcccgagatg cctgcgagca
     1561 cgatgccggc gatgagctcc cccgccgagg agagaaacag cgccgcgaac ccctgcctga
     1621 gactctcttt ctcctcagtg aggtaaccgg agaccttcgg gcgcaggcga ggcctgttgc
     1681 gcagcctgtc gcgcaccccg ccctcccggc cgcgctctcg ccaccggacc cgccttttct
     1741 cgctctcgga cacggggaat gtatagtcgt tgcggaccgc gaagtaaagc cacccctcgg
     1801 gggcagatgg cagcgcgcag tctggctcac cgatctataa catacctgca gaggcctgat
     1861 cggcgcattg ggaagtcgct cttgcttcgc aggcgccgcc aacgaacgga aagggagaac
     1921 ctatgagtgg gatcagagcc gtagtcgtcg accccgacgc cccgaaccgc ctggcactgg
     1981 cggacgtgga agagccgtcc ccaacgcctt cggaggtcct ggtgcgcgtc tcggccgtct
     2041 cgctcaaccg gggcgaggtg cgccgggccg agtccagcga accgggattc cgacccggct
     2101 gggacctggc aggcaccgtc gaacgcgcgg ccgctgacgg ctcgggcccg cgggaaggct
     2161 cgcgggtcgt tggcttcctc ccctcaggcg catgggcaga gcttgctgcc gtgccgacgg
     2221 actccgtggc cgttgtggcc gatggtgtgt cgttcgagga ggcttcgacg ctgcccgtcg
     2281 ccggcctcac ggccctctac gcgctggagg aggggggcaa cttactcgga aggaccgtgc
     2341 taattacggg cgcctcgggc ggggccggac agttcgcgtt gcagctcgcc cgtgcggccg
     2401 gggcgcgggt ggtcgggctc gtgcgccgcg aggagcatgt gagtctcgtg gaggaggccg
     2461 gcgcccacga ggtcgtcgtg gacgagagcg gcgaggcggc cggggagaag ggcccttacc
     2521 acctgatcct cgaatcggta ggaggggagg tgctcgggaa cgcgctctcg atgctggcgc
     2581 cgggcgggac gtgcgtaagc ttcggaacgt cggctacggc cgacgtggag ttcgacgcca
     2641 ggacgttcta cctgacgggt ggggcgaggc tctacggttt tatcctgttc cacgaagtgc
     2701 tggcgagacc ggcctcgcaa ggtctccata ggctggcgcg gatggtcgcg gatggaatgc
     2761 tgaagccgcg catcgaggtg gaggcgccgt ggacggagct gggagaaatc gccgctcggc
     2821 tcctagagag ggggtatacg gggaaggcgg tgctgcgcgt cagcggcgct taaaacggca
     2881 tgcgaaaggt caatcctttc gcatgccgcg acttcaggtt tatctggagt atttgctctg
     2941 cgtaggcagt tacgtttgtg ctaacgggct tggcgccccc gaagcatgag ctttcaggct
     3001 ctcttcggct acgcctatag cctcctcgtc cagcttggcc tcttgggcca tgttccatac
     3061 ctgggagatg attaccggcc agaaaaccag cgcggtgata atcgtaggga tggcctgctg
     3121 accgaagatg cctacgcccg cgtccacctg ggtcccgcct tcgacgggct ctacctttat
     3181 gtttagggcg gtcttcatgc ctatgatggc cttgaaggtg ccacccttgc gaatgctgac
     3241 ctgcacccct cccgcgggga ggttcgtttc ggtagtctcg aagtcctggc cttcgaagta
     3301 gcgcaccaaa tcctgggcaa cagggccgag gtcttcaaca acgtagggta cggtcttagt
     3361 ggaattgaag gttcccacct tgaatctctc ctttcagaaa aggggtaagt gccatgccgg
     3421 tatcttctcc ttagtccctc tttaggacct ccttcaccac gttagcattc tcctgatcca
     3481 acattagcat gtgcccgggc tagagacaaa cccatcaaag atctggaacg catgggaccg
     3541 ttagcggccc acgcgtttac ggtacgagcg ctagtcgaag tcaggtagta agttcgggtc
     3601 aaggtggtgc tcgaagaagc gtgagacctg ggccggggta acgcgggtga ccgagagctc
     3661 aggaagctca tcctcgccga agaagcgcac cccgtcggta tccacgtcgg acaaaggcat
     3721 ctcgtccagg atctcgcaca agtacaccag cttgtagacg tggtagggga tgggcgggtg
     3781 gccgtgcctg tcgcggtcgt acgccgcgag aagcctgacg ggccgtaccc tgtagcccga
     3841 ctcctccagg gtctccctgg ccgcggcttc ggacggggat tcgcccacgt ccgcccaacc
     3901 tcccggcaag gaccaacccg gttcgcccga ttctttcacc agcaggatcc ggccatcctg
     3961 aaacaccgcg gcccggacgt ccaccttcgg ggtcgcagga ccggtttcga gcgcgagtag
     4021 atctctggcc tcttccaggg tccccgtgga gtggcccgcg aatatctcgg ccgccagctc
     4081 ccttagctgc tcgtatcgcc cgagatcata atcgtctctc gaataggtca ggccttcttg
     4141 ggcaatggct tgcaggcgcc tggcccaccg gagccactgc gggtcggggt acttgtcgct
     4201 atctccagag gctatgcttg gtggctttct tttgcggaat ctagccttgg ttcaacgtag
     4261 ttctgtcccg ccttccacga ttatgggatc cagccctgcc tctatttcct ccctgcgctc
     4321 ttccaggaag ggagggagtg agagtctctc gccgaggtgc gccgcgtctt cgtcagtcgc
     4381 gaaaccggga ccgtcggtgg cgatctcgaa gagcaccccg ccgggctccc gaaagtagat
     4441 cgaacggaaa tagaagcgat ctatctgcgg cgttaccccg agtccggaac gccgtatctt
     4501 ctccctccac gccgcgtgct cctcgtcgtc gggcgtcctg aaagcgatgt gatgcacgcc
     4561 cccacgcccc aggtgggtcc acggtgcgtc ggggcgttcg atcacccgca ccgtcgcccc
     4621 cggacctccg ccgccgacct cgaaggtcac cgtctggcct ccgttctgta cgaccgcgct
     4681 cacccttcgg aagccgagca cttcggtcag gacccacgcc gtgggctcga gttcccgaac
     4741 cgtcagatcg acggctccga gaccccggat ccccatctcg cgcggaacgg ggctctcctc
     4801 ccacggatca cctccctgta ctccttcgcc ctcatcggcg gagagccgaa gtcgctgccc
     4861 ttcgggatcg gtgaagtcga ggtaggagtg ctcttcagtt tcttcgatgc cattgttatc
     4921 cacgcccaga tccctgaacc gcgcggccca ccagtcgagc gcggcgcggt cggggacgcg
     4981 caactcggtg gtcgagacct cgcccgcgcc gggatggttg ggggccacgc gcaagtgagg
     5041 ccaatcgaag aaggtgatct cggtcccagg attacccacc tcgtctccgt agaagagatg
     5101 gtaagcggag acatcatcct gattcaccgt cttctttacg agcctcagcc cgagcacgtc
     5161 cgtgtagaat gccacgttct ccgaggcgtt cgtcgtgacc gccgtcatgt ggtgcaggcc
     5221 gcccagttcc atctctatcc tcctatcggt cgtctcatca agctcggggt acaagggaac
     5281 gcgcaccgcc gcttatacgt tcggcaggaa gcccctgtac cgtgtgctgg tagtaaaaca
     5341 ggtccacatc cagcggggcg agcagcgccg tagcaaggta ctcgaggtct agaccaccgt
     5401 tcacgtctcc ctcactgacg ccccacgact accgctctcc accaagacct cgacaccgcc
     5461 agtctaccgc gctaccctcg cactttaaag cggacgagcg tccgctttta atgagatagg
     5521 cggtgaaacg acgagagtga aggaggaaga gaggagaacc cggttctcgt ctccttcgaa
     5581 cagtacctcc gcgccaaagg ctaggagaac gccgggtctc aagagggctg ataggtgatt
     5641 ggataaatcg tttcccctgc atagtgcgca acgtctgctg cagtggattc ccgcttgcgc
     5701 gggaatgacg ctcatggtaa aggcagagag ttgcgctagc cagcgtgaag tagcgtgctc
     5761 gaccggagcc ggttgtagct gtacgatggg agtgtgagaa gcggtaccca caccttcctt
     5821 cagaccacgc gcgtccattt cggcgcggac gctctggaga agctcgaaga ggaggcctgg
     5881 tcgtacgacc gggccttcgt cattacaggg cgtagcctga acgagaagac aaacctcgta
     5941 cgccgcgtcg aggcattgct cggtggcagg cacgcgggga ccttcgccgg tatcgggcag
     6001 cacacccccg gtggggcggt cgagcaggcc gccgtcgggg cggagatgac ggcggccgat
     6061 ctcctcgtga gcgtcggagg gggcagcgtc atagacggca ccaaggcggt ggcgcgggag
     6121 ctgggatatc ctcagcaggt ggcgatcccg accaccctct ccggggcaga gtgggccaac
     6181 agggcgggcg ttacggacga gagatccggc aggaagagag gtttcgccga cccgaagact
     6241 gttcccaggg tcgttgtgct ggaccctgag gcgacggtct tcacgccaga gaagctctgg
     6301 ctctcgaccg ggataagggc cctggatcac gcggtcgagg gctatctcta cgggggcgat
     6361 catcccataa ccgacgtaac cggcctcgaa ggggcgcggc gcctgatgga gtatctgccg
     6421 ctttcgaggc atgagccaga cgacttagat gtgcgcctgg aactgcaact ggcctcctgg
     6481 ctggcgtact tcgggccggc gaacacgccg atggggctat cgcacgggct ggggcgcagg
     6541 atcggggcca gctacgaagt cccgcacggg ataacctcct gtatcaccct tgcaccgagc
     6601 ctgcggctcg ccaagaagcg cgtacaggac gtgcgatgga gcaagctttc ggaagcactc
     6661 ggcggggagc cgtctgagcg cgtttcatcg ctggtccagg agctcggatt gccgggacgc
     6721 ctgcgcgagg tcggagtacc cgaggaggac ctcgaatcta ttgcgggtga gtacggcgat
     6781 aaggccgggg aagcgctggc gatcctgacg gccgcgtact aggatgcgcg agccatgatt
     6841 cctgcgagac tcgagcgggg cgatgaggtt cgggtcgtct caccggcggt cagcctcggt
     6901 ttcataccag aagagcagaa gctgatagcc agagaaaggc tcgaagactt cggcctccga
     6961 ctctcgtttt caacgaatgt aggggtgatg gatcgcttcg attcctcacc cgttcaggcg
     7021 cgagtctccg atctgcacga ggcgttctcc gatcctgagg tcaaaggcat actcacgacc
     7081 ctcggcggct acaacagcaa cgggctactc gggtatctgg actacggtct tttccgtgca
     7141 aacctcaaga tcttctgcgg tttctcggac attacggcgc ttgcaacggc gatacacgcc
     7201 aggaccggcc tcgtcaccta ctcaggaccg catttcacga ccttcggcat gaaacgcggc
     7261 atcgaataca cagaggaata tttcgagcgg tgcctgatgc gtgggcagcc cttcgaagtc
     7321 cttcccgctg accactggag cgatgacctc tggtaccagg accaagagga tcgtgaattc
     7381 gtacccaacc ctggctacga ggtcatgtac gaaggcgagg cggagggcaa actgctcggg
     7441 ggaaaccttg gtaccctgtg cctgctcttc ggcaccccgt atatgccaga cctggagggt
     7501 tccatcctcc tcctcgagga cgacgaggag gtcaatgcag ttcacttcga cagggcgttg
     7561 caatccctca tccaccaacc tggcttcgag ggcgtgcagg gcatggtatt cgggaagttc
     7621 cagagggctt ccaacatgga ccaggacacc ctgacggcca tagtcgagag caaacgggag
     7681 ctcgaccgaa tgcccatcgt cgccggtgcc agcttcggcc acaccacacc gcagttcacg
     7741 ttcccgatcg gtgggtacgg aagattgcat gctggcgatg gcgctgccag gctatctatc
     7801 aacgtccatt gagttcgtat gggggcggtt cgcgaaccgc cccattaggt tcgcgaaccg
     7861 cccctacagc tccaggcgcg actcttccag gtcgaggtcg cgctctatgc ggtgcatgat
     7921 ctcgtccccg atactgcctt cgtcgcgtag agtgacgagt ctcaggcgct gcgccgcgag
     7981 cagctccacc atgaggcgct ggtaggcggc agagcgttct tctacgcctt cgggatctcc
     8041 atcgaagcgg gtggcgaagc ggttgcggcg gtaggtgtag aggccgcgaa cgcgctccgt
     8101 cgtgtcttcg cgcacccagt cctcctcgac gagctcctcc aggcgttgta gggcggcctc
     8161 cgcgacctgg atgcggccgt gggtctcttc cttatccccg atgtggtcgt cctcgaggcc
     8221 gagggcccgg atcagtaccg gcagggagag gccctggacc acgagcgtcg ccaggatcac
     8281 gcagaacgtc aggaagatga tgaggtcgcg ggcggggaac gctaccccct cggccgtctg
     8341 aagcgggagc gcgagggccg ctgcgagcga gataacgccc cgcatcccgg tccacgctac
     8401 gaccgagacg ctgcgccagg gcggtgaggg gtctcgctcc ctgatacggc ggaaggccca
     8461 tcttgggaca tacgtagccg ggaagaccca gataatgcgc acgaggatga cggtcacgct
     8521 caccagaacc gcataggaga tgatctcgcc cgccgtgaac tcgccgagac ccccgaccac
     8581 gctcctgagc tggagcccga tcaggatgaa gatcagaccg ttcaaaagaa acgttatcag
     8641 ctcccacgtc gcatacccct gaaggcgcgt ccccgatgag attatgtagg gtcctttgcg
     8701 cccgagatac aaccctgtgg ttaccacggc gaggactccg gagaagtaga gctctcccgg
     8761 cacaccgaac agctcgtgca gcatctccca gacgctgtgt ggcagctcct cggccgccag
     8821 gtaggcgacg aagggcgtga gcagcgagac gatattctgc acggaggggt cttctgagag
     8881 gtggcgtcgc gcccaaataa cgagccagcc taccgcaaag ccggcggcga tcccgcccgc
     8941 cgcgccgacg acgaactgca gacctgcttc ccagatcgag aaggcgccgg tgaccacagc
     9001 ggctaccgcg acacggtaag cgacgatgcc cgtggcgtcg ttgaccaggc tctcgccttc
     9061 caggatcgtt acgatgcggc ggggcacccc gaggcgctgg gcgaccgcgg tggcggcgat
     9121 ggcgtcggtg ggggagacga tcgcccccag gacgaaggcc gccgcccacg gtatcccgga
     9181 gacggtgtgg acgacgacgc cgaccacgaa ggtcgtcatc agtacgagtc ctacggcgag
     9241 cagcgagatc gcccttatgt tgccccgaaa gtcgcgccac gaagtaaaaa tcgccgagac
     9301 gtacaagagc ggtggcaaga acaacaggaa gacgagctcg ggatccagcg caacctgcgg
     9361 cagcccagga acgaacccga gcgccgatcc gccgataacc agcaggatcg ggtaaggtat
     9421 cttcagccgc gtggccagcg tggccaacgc cgccaccgca aggagtagac cgaggattat
     9481 ctcaatctga tgcaacgctc acgtccattt cagctcagac aaagcgctcg gtcataagct
     9541 gcgtaacata ggcataccgg ccgagctcgc ttaggagtcg ttgtacccgt tcgctctcca
     9601 tttccctgcc caactgttcc aggtcgttga ttccgaccga aacgacatac tggtagggcg
     9661 ggtcaccgcc cgattccgga agaccgccca ccctataacc gcgccactcg tcaaccgagg
     9721 cgaggtccag gatcgcgggg acgtaccgtt cctggagcca gcgctcgtat tcttcgggga
     9781 ccacatcttc cttcagatcg accagcacta tcatcgtcgg caccggtgct cctcccttcg
     9841 aactccttgg gtcagaccaa cacctcgtcg acgtagcacc accgccagtc ttcacccggc
     9901 tcgaaggacg tgattatggg gtgcccggtc gcgtggaagt gggccgtggc gtggcggttg
     9961 ggactctgat cgcaacagcc aacgtgacca cagaccaggc actttctgag gtgtacccac
    10021 gcatcgcccg tcttcagaca ctcctcacaa ccctgcgccg atggctccac gtcccttatc
    10081 tggtctatgt ggtcgcattg ctgctggaac atttgcatta acctccgcat gggtgtcctc
    10141 ctcaggctgc tgccgtctct ccctcgggtc ggcctgcctt ctctctcggg gcggtgctcc
    10201 ccttgccctc gattatcacc gcggctgcga ccgcgctcgc gacggccagg cccgcggcta
    10261 cgaacatcgc gagcctgaag ccggccagaa acgactcctg gatggcgcgt tcgatctcta
    10321 ccgccgtccc ttcgcttact tctgcaggta cctgcgcccc accgaggttt actttctcgg
    10381 cctcgagctg ctcttgggct tggggtgaga gatccaggtt ctgcaccctg gcgtcgaggc
    10441 cgccggagaa gaccagaaag acgaagatgc cgagcacggg gatcgcgagc agccccgcgg
    10501 ttctggagac ggcattgttg acgccggagg ccaggccgga atggctgccc gagaccgagt
    10561 tcatcgcggt cgtcgtcagc ggcgctatca cgagcgccat cccgaggccg agcaccacga
    10621 tcgcggggaa gaacgtcgtc cagtaggaac ctccaatccc gggcagggcg aagagcacga
    10681 agcctgcggc ggctatgacg gggccgatca tcaatggcag ccgcgccccg aaacggatga
    10741 ccagaccacc cgcccaccgg gacatcacga acgttatgat cgtgaagggc aagaacgaac
    10801 tccccgccgc cgtggccgag tagccgtgga cctggatcag gagaaagggc aggaagtaca
    10861 gtgccccgcc gagccccgcg tacaacagtg gggtaaggat gtttgccccc gagaagttcc
    10921 tggaccggaa caggctcaaa ggcatcatcg gctcctccgt ccgccgctcc cttacgacga
    10981 aagcaacgag cgcgccgacc ccgagcacga gcgggagtat caccctgagg tcggtaaatc
    11041 ctgcctcggc agaggagatc aggccgtaca ccacgccgcc gagaccgatc gtcgcaaacg
    11101 ccgcgccagg gatgtcgagc tctctcgcgt cggggtcgcg gctttcgggg acgtacctgg
    11161 cggtgatgag cagcaccgct acagcgagcg ggacgtttat caggaaggcg gcccgccacg
    11221 agacgttctc gaccaggaac ccgccgagga cgggacccag ggcggtcgtc acccccgaga
    11281 gccccgacca cgtgcctatg gcctttccgc ggtcttcttc atcgaaagag gcgcttatga
    11341 ttgccaggga accgggaacg agcatcgcgc ctcccacgcc ctgcgcggcg cgggccacga
    11401 tcagcatctc ggggctggta gagagcccgc accacaccga ggcgagcgtg aagacgatca
    11461 cgcccaaaga gaagatgcga cggcgtccga aatggtcccc gagggaaccg ccgaccagga
    11521 tcagggccga caagaacagg gcgtaggact cgacgatcca ctgcgcgtcg gtcgccgtcg
    11581 ccccaaggcg ttcctgtatc tgcgggagcg cgacgttcgt gaccgtgctg tcgataaagg
    11641 ccatcgccga cccgataatc gtcgccgcga gcacccaggg acgcgacgtc tgctcgcacg
    11701 gcgcatcggc cctatgagag cgtatgaccc ctacgtcgca cggaggatta cctatgttcg
    11761 acaagctgat cctctctggc cggcggccat tcagctcaga acgaagagcc ctcggtcttc
    11821 aggaactcgg cctcctcggg cgtagtctcc ctgcccagga tctcgttcct gtgcgggaac
    11881 cgcccgaacc gctcgacgat atctctgtgc gcggcggcgt aggaggttac gtcgggcgcc
    11941 cctggctcgt ccgcgagcag cccgaacagc tcgacggagc ggcgttgatc ctccctgttc
    12001 tcgctgtgca tgaacggcat atacaagaac atcctctgga aggcggggag ctccctgtct
    12061 agcgcgtgct cgacggcgta cctggaggac tccagcgccg ccccgtcggc ggcgtgggtg
    12121 cgtccgtcgc cacggaacat gttgcggggg aactggtcga gcacgatgac gagcgccagg
    12181 cagctccttg cgtcgtcgcg ccagccgtcg aggtcgccgg ccacggcccc atcgtagaga
    12241 ccggcgaacc ggtccgttac cgtggcatcg aactcggggt ctttgcggaa ccactcgtcg
    12301 cggaactcgc cgtaaccggg ctcgccttcg cgcccgaacc agaagtcgag tacttcgtcg
    12361 gccctactcg gcacggccta tcaccgttac gtcttcgtcg cggcgggtga agatgcggcc
    12421 ctccatccgg ctcttgtagc cgtctatcat ccagagctcg ggcacgatct cggagaccag
    12481 ctcgccgtcg tcgaacatcc tgaccatcga gctctcggag acgacaacgg caactgccct
    12541 cgtctggcgg gagatggatg cgcccgccac gtgacggctg cccaatccga gcggcaggtc
    12601 caggttgtcc gaggcggcgt ctatgtaacg ggccgccgag agtactacgc ccctgttcga
    12661 gaccaggaag gccccgtcta gctgggccag ttcctttatg gtctcccgca tgtcggggtc
    12721 ctctatctgc ttgctctcgt ctgggtgccc gtgcaaaggg tcgaggatca agggttttga
    12781 tctctttgtc acctcgcccg agtcgccgac gacgaagagc gtcccgatct tgcgcccctc
    12841 acggccttcg cgcgctatct cgaccgcgag cgagacggtc tcctcgagca cccgcctgtc
    12901 tacttcgcgc ttctccgtgc agatctcatg aaagacctgt gtttttccca ctgagatcaa
    12961 cccctgttct gtctggtatc gataggtaac cttaccgtga agcatgtctc ccctggctct
    13021 gactgcacga agatctcgcc tccatgatcg gtgacgatgc gccgcacgat ggccagcccg
    13081 agcccggttc cgtccccgat atctttggtg gtgtagaagg gctcgaagac atggacctgc
    13141 gccgcgcgcg gtatccctgg cccgtcgtcg gcgacctcga cgaccacccg cgcgccttcc
    13201 gcgtacgccc tcacggtgat acttccgcgt ccgtcaacgg cgtctatggc gttgtcgatc
    13261 aggttcgtcc acacctggtt cagttcgcct ccgcgccccg gtatccgtgg cagggatttc
    13321 tcatactcct gcgacaacga gacgttcttc agcctggggc cgaggatggt gagggtgttc
    13381 ttcaggccgg agatcacatc tacctctccc acagccgccc tgtccatgta ggtgtactcc
    13441 ttcatggccc cgaccagctc ggagatacgc cctgcgctcg tctcgatctc acccgccagg
    13501 cccacgagat ccagcgtccc ggcgagccac tccagaccac aggcgagcga gtcgttaccg
    13561 aatgcttcgg ccatcgcctc cagacggtcc ttcttcacgc ccgcctccgc caacgccggc
    13621 gccagatccc acgagtcctc acagccatgg tcttccagcc aatcggcgag ctcatcctca
    13681 gcgtcgccaa gagagagcgg gtcgagaaga tctgcggtgc ccgcggccgt ttcacgttgc
    13741 agggcaacga gtgcctctcg ctcgggggcg gagaagcgct cgtcgtgccc gatggcggag
    13801 agttgggctt ccaggatcgc cccgcccaac tcttctgagg cccgcctcgc cgcggctgcg
    13861 ggattgttca gttcgtgggt gaggccggcc gagagtttgc ccagggccgc gaacttctcc
    13921 tgctggcggt aggcccgctg ggtgtagcgc atgcgcctgg ccaggccaga gattaacatg
    13981 tctgccacgt ctgggacctc gacgggcaag cgcctgaagg tctccgagtc gatctccaga
    14041 acgcggcccg gcgcggccgc cctcgccctg tgtatcgaag tcctccccgt cagcatcacg
    14101 agcccgccgg tgaactcgcc tgccccgtgg ttcccaatcg ggatctcctc agcgccgtcg
    14161 aggcgagaga tctcgacctc cccttcgagc acgatgtaga agctgtccac gacggacagc
    14221 tcgtcgaaga ggaggtctcc ggtctgcagg gcgaactccc tgccgtaaga catcagcgcc
    14281 tcgatctgct cgggtgtgag agcctgctcg tctcgagcat cacgcatcat gagtgcgccc
    14341 ccgtcgactt gtctaccggc agccgcactt ggaagcgggt gtcgccgggt tcggagagga
    14401 cgcggatatc gcccttgtgg tcctcgacga ccacgcggta ggagatatcc aggccgagcc
    14461 ccgtgccctc gccgacgtcc ttcgtggtgt agaagggctc gaagatcctc ccgcgcacct
    14521 cctctgggat gcctggaccg tcgtcggaga cctcgacaag aacccccccg ttctcgctgg
    14581 cggtcctgac cgtgatgcgc ccccggccgt caacggcgtc aatggcgttg tcgatcaggt
    14641 tcgtccacac ctggttcagt tcgccggcgt ggccgcagac gtgggggagg tccttctcgt
    14701 agtcgcggac gacttcgacg tcgcccttct tcagcttgtg accgagcatg atcagggtgc
    14761 tgtccaaccc agcgtgcacg tcgactctct tcgaggccgc tctgtccatg tgcgtgtacg
    14821 tcttgatcgc cccgaccagc tcggagatgc gcgcggatcc accctcgatc tcccgcagca
    14881 actcgtcacc ggccaccgtc gcccgcaccc agccgagcac gccgccgagg gcctcgtccg
    14941 gcaacctctc tgcgagtgct tcgagccaga ctgcgtccag cccggcgccg gctagggtgg
    15001 gggagatctc ccacccctcc tcgacgccgt ggtcctccat ccacacggca agctcgtcct
    15061 ccaggtcgct aacttccagc gagtcgagcg tggtccctga cttctggctc gcctcttgtg
    15121 gcagcccggc gacgaaagag cgctgttctt cggtgagggg gagattcccc agctccacag
    15181 ccttctccga ggcttcctgg aacgcctctc tggcttcggc ggcgctacgg cctatggcgg
    15241 cggcggggtt gttgagttcg tgggcgagtc ctgcggccat ggtgccgagc gagatcagct
    15301 tgccgtgttg ctgggagacg gactcgtgga gttcggcgcg ttcgacggag gttgcgagga
    15361 tgtcacggag gacctcgggg cagatcctca gcatctccca gaaggatggt acgtcgagct
    15421 tgaggaggcc gacatcggtt atggcgtgtc cggtcgtggg ataaggggcg tcgagcacca
    15481 ttatgagctc ggcgaagata gtaccctccc ctagcgtgac gacgaagacg tcctcctggc
    15541 cgaccctgcg ggtccactcg gtctcgcctt ccatgacaac atagaaacca tcaggaggat
    15601 ctccctggcg tgcgatcagg gtccccgcat ccagcctgac ctcctctgcg tggtcgtgga
    15661 tccaggccag cttctcgtcg gacagtcgcc tgaagagcgg caccgagcgc aggcaatcga
    15721 ggacccgctc gcccggcacc tcagacctcc ctcaggtact ggtggacgaa ctggatggcg
    15781 atggagccct cgccgacggc cgaggcgcag cgcttgaccg agccgtggcg cacgtctcct
    15841 gcgacgaaga tgccaggcac gctcgtctcc agtaggaagg ggtccctatc aggccgccag
    15901 cccttgggac gcttcccctc gtgcatcacg tccggccccg agaggataaa gcctcgttcg
    15961 tcgcgctcga cgacgccgtc gagccacccc gtcttcggcg ccgccccgat aaagacaaag
    16021 agcgaatggg ccgccgtggt ctcggtctcg cccgttaccg tattcttcgc ggttatccgc
    16081 tccaggtggt ccttaccctc gacagagacc acgttggtgt cgaggagcac ctcgatattg
    16141 tctctggctt cgatctggcg caccaggtaa tccgacatgc tcttcctgag gtcgccaccc
    16201 ctgtagagga taaataccct gctcgcccgg ttggagaagt acatcgcggc ctggccggcc
    16261 gagttcgcac ctccgacgat gaacacgtcc tcgcctacgc aggagagagc ttccgtctgg
    16321 gcggcgccgt agtagatgcc gcgtcccgtg agacgatcaa cgccgggcac atccaatttc
    16381 cggtaggaga cgccggtcgt gatgatcagg gcgtagcacg agatctccgt cccatcaccg
    16441 agggtaacga tgcggtaagg gtctttcacg cgcaccgcgc tgacttcctg gggtgtcagg
    16501 atctccacct cgaagcgctt cgcctggtcc acagccctcc gcgccagatc gccgcccgaa
    16561 agtccggcag ggaaacccag atagttctcg atgcggctgc tcgttccggc ctgtccgccg
    16621 ggcgcctcgc gctctatgag gacggtcttc aggccctccg aagccccgta gaccgcggcc
    16681 gcgaggccgg atggtcctcc gccgacgatc accaggtcgt agaattgctg cccggcgcgg
    16741 gtctgaagcc cgatcttgtc ggctatctcc ctgttctgcg gcgcctctgc gtaagagccg
    16801 tcctcgtaga cgacgagcgg cagcttcggc gcccccgaat cggcgttcgc aacgatctct
    16861 cgtgcctctt cggacccctc tatgtccagc cagcggtaag gcacgcggtt gagggccaga
    16921 aactcccgcg tgcggtgcga ctcgggcgac cagcggtcac cgacgacccg caatcccgtg
    16981 aaaggcggcc tgtagtcggc ccgccagtcg gcgagcatgt cgtcgaggtt ggggtagagg
    17041 ttctcatccg gcgggtccca cggtttctgc aggtagtaat cgaggcccac ctcgttgatc
    17101 gcacgaatag cagcctcggt atccgagtag gccgtgagca gggcacgctt ggcctcggga
    17161 taacgctcga tggcgtgctc caggaactcc acgcccgtca tcttcggcat ccgctggtcg
    17221 accagaaaca gcgccacggg gtcgccccgc agcttgagct tgcccagggt gtcgagcgcg
    17281 gactccccag agtcagcccg aagtatcctg tactcgcgcg cgtaccggcg cctgaggtcc
    17341 cgctccacgg cccgcagcac ctgagggtcg tcgtccaccg ccaggatcac cggcttcgcc
    17401 ataacgcccg cccttcaaat acgatcgtca aatagtcttt ccatcccgca aatcgcgccc
    17461 ggtgtcactc cagacgcccc aaaaaattct aacatcccag gcaacctggc cgttcgggta
    17521 ggttattact ccctgccgtt ttggggtccg gagagctttg gtaggacgtc cgagagccac
    17581 gccttcgcct cggcgacatc ttcgtaggtc aggggatgtc ctgcgtcgcg ccagcggagg
    17641 tctacgtctg cgcctgcttc gagcagtatg tcagcgaggc gctgcgtgtt gtctggtggg
    17701 atcatgcggt ccatccgacc ggctgctatg aagacgggca tccctgagag atcgggcgtc
    17761 acctcgggct cgaacggtac catggccctg aacaggaccg cggcccggag tagaccggga
    17821 tgcaacaaga tcgtgctggc ggccacgttg gccccgttgg agtagcctac ggccacgacc
    17881 ttcgatgcat cgaagccgta ctcttctgag gcggcttgca cgaactcggc cagctcgtgc
    17941 gtgcggaaca acaggtcctc gtggtcgaag actccttctg ccaggcgccg gaagaaacgc
    18001 ggggcgccgt actccgagac cttgccgcgg ggtgaaagca aggcgccgcc gggggcgagc
    18061 gtctccccga gtggtgcgag atcttcttcg ttaccgcccg tgccgtgcag caagaggagg
    18121 gtcgggccgg attcatcttc gccgggcaca tagcggtgga cgaagccgag gtcggcgctc
    18181 atgcgttctc tcctaccggg acgcgcagcg acgggaggac ctcttccagc ctgtcgcgac
    18241 gggtctcgag ccagggcggt agcttgaggt cttcgccgag gtgttcgggg tcctcgtcta
    18301 ccgtgaaacc tgggggatcg gtggcgatct cgaagagcac cccgccaggc tcgcggaagt
    18361 agatcgaacg gaagtaattg cggtccagga cgggcgtaac actgtagccg agcgccgata
    18421 tctccgtgcg taccgcaagc tgggtatcct cgtcggggac ccggaacgcg acgtggtgca
    18481 cggtgcctac gcccatgctt ccatggggga agccggccgc gttcaccacg tcaacgagat
    18541 ctcccgggcc acctttaccc gaggcgagcc tgatcctacc ctccgcctcg tcgacccgcc
    18601 ggaagcctag aaaatccgtc atcagcccgg cggtacgttc tggcgcttcg acggcgaggg
    18661 cgacgtggtg gaagcccctg acggagtgat cggcctggac gggaccgccg gaccatcccg
    18721 ggcgtccgtc ttccgcggcc acgagctcta tgcgcaatcg gtccgggtcc tcgaaagtga
    18781 ggaccgtatc gccgaagcgg tcgcggggcc tctcgaagcg gacacccttc tccaccaggc
    18841 gctccgtcca gtagcccaga gacgtggtcg ggatggagaa ggaggtaacg acgagctggc
    18901 cggcacctat gcgtccccct ggcgcgtgcg cccacgggaa gaacgtcatg atggtgccag
    18961 gactccccgc gccgtcccca taatagaggt ggtaggtctc gggatcgtcg aagttgacgg
    19021 tcttcttgac cagccttagg cccagagtgc cggcgtagaa gtccacgttg cgctgcggtt
    19081 cgcccgagat ggctgttacg tggtggatcc ctgtagtctg cgccataatg ctcctcttcg
    19141 cgctttcatc ggacgcacga tcatcttaca gcgtcgcgca gaaaggctga acccctctgc
    19201 acgacgatct atcagcactc agctcttcgg ttgctagaag ctgaaggctg atagctgacg
    19261 gcgccattct cggaaaggtt caacctttcc gagaatggca ctaatcgacg tgcggataga
    19321 ccaggacggg ccctctcgcg gcggtcacga ccttggtcga gacgctgccc agcctggtcc
    19381 ggatcagtcc ggcaagtccc ctgctgccga cggccacgag cgagggttgc acctcctgag
    19441 aggcttcgag aaggacattg gccgggtagc cgcctgagat caggatctca ggcttgctac
    19501 cgagcgtctc ctccagcgcg ccggcgcgct cctcgagcat ctctcggtcc cgctctcgca
    19561 tatctacgag gttctggacc ctggacctcg tctccccggg aggaacctcg ggcaggtcgg
    19621 gatgggagta gacgagcaga ccctcggcat cgtagagctt gccgatgccg gtggcgagtt
    19681 ccccggcctt gcgcgcgtcg tcggagaagt cctcgccgat gacgatccgg gccggaggcc
    19741 agaaatcctg ggcgcccgtc agtacgagca cggggacctg ggcgtggtgc acgatctcct
    19801 cggactggct acccatcagg atgcgccgca ccgtgcccag cccgcggctg ccgactacca
    19861 ggagcccggc gtcgatctcc ttgcagagcg cgatgacctc attggaggtg cgcccactcc
    19921 tgagatacgc tttcgcgact tccccgccgg aggccttgat cctctctacc tgctcgtcca
    19981 ggatctcctg cccctggcgc ctcaactccc tcttgacgaa gtcgtgggcg aacccccgca
    20041 catcgtgcca gacgtgcacg acgtgaagct cggccccgct ccttctggca aggtccgagg
    20101 ccgcctccgt cgccctgata gcgtcctccg agccgtcggt aggcaacagt atcctctccg
    20161 gaagcacgct cattcgaagc tcctctcatc ctccatgata ttgaacacca gcccgaataa
    20221 taccgccgtc cgcgctttcg ggctgggtca ggatcttccg cccgagggga cgggctcgcg
    20281 gaactggcgg cggtagaggt cggcgtacag tccgttcttc cccaggagcg tcgcatggtc
    20341 gccgcgctcc gcgatgcttc cgtcctgtat gacgaggatg aggtcggcgt tcctgatagt
    20401 gctgagacgg tgggcgatga cgatgctcgt cctgccctcc agcagcgtgg ccagggcctt
    20461 ctggatgatc gcctccgtgc gcgtatctat gttgctcgtc gcctcgtcca ggatgaggat
    20521 gcgtgggtct gtcaggaccg ccctggcgaa ggagagtagc tggcgttggc cctggctcaa
    20581 tgccccgcct ccctcgccaa ggaccgtgtc gtagccctcg ggaagggcca tgatgaagtc
    20641 gttcgcgttg accactcggg cagcggcctc cacgtcttcg cgcgtcgcct ccgaagggtc
    20701 gccggaacgg ccgtagccga tgttctccgc gatggtgccc gagaacagga aaggctcctg
    20761 gagcactatc ccgatgttgg ccctgaggtc cttgcgggcg agctccctga cgtcccttcc
    20821 gtcgataagg accctaccct ccgtggcgtc gtagaagcgc ggtatcaggc tcgctatggt
    20881 cgtcttgccg gcccccgtcg ggccgacgag ggccaccgtc atcccaggct cgacccggaa
    20941 atccacgtcg tgcaggacgg ggttgccggg gtcgtaggcg aaggtcacgt gctcgaagga
    21001 gatgcggccc gtggcttgac cgagtttcgg ggtgtcgtgc ggatcctcgg gttcgggttc
    21061 ttcgtcgagg atgttgtaga tgcgctcggc ccctgcgacc gcggcctggg cctgggtgta
    21121 gacctgggag gcgagctgta tcggccggaa gaactgctgg acgtagatca aaaacgcggt
    21181 gaggatcccg atggagagcg agccggcgaa gacgaggtag ccgccgtagc ctatgacgag
    21241 agcggtagcc agggtcgaga gcatgtctat ggtgggggcg aaggccgagg tgatcgccac
    21301 ggcctgcacg ttggcgtcgc ggtttgcggc gttgcgccgc ctgaaacggg ctatattctc
    21361 ctcggtcctg ttgaaggcct gcgcctcgcg caccccgacg atgtcttctt gcactcccgc
    21421 cgtcacgtcc ccgaccgtct cgcgcgtaag gcggaaggcg cgccgcgcgc ggcgggcgaa
    21481 aaaggaggtc aaaacgagca tgataggtat gatcgtgaag caagcgaggg cgaggttcca
    21541 gtcgaggaag agcatcgcca cgacgatccc gatcaggctg aacagggatc ccaacagctg
    21601 ggtgagcccc tgtgagaaga gctggttgag ggtgtcgacg tcgttggtca cccggctcat
    21661 caaatctccg acgggcctcc tgtcgaagta cctgagtggc agatgctgga gccggtcgaa
    21721 gatcctggcc cgcatggagg ccagaagact ctgcccgata gagccgacct ggtagacctg
    21781 tccccgcgag gcgatggccc ccacgacgta gacccctagg agcaccagca tgatcctgga
    21841 gagcccggcc aggtcgccgc gcaggatgta gcggtctatg gcacgaccga tcaaccaggg
    21901 ccctccagcc tgagcggccg ccccgagcac gactagcccg aagacgatca tcagcttgcc
    21961 cgaatagggg cgcagttctc ccagcagacg ccttgtggta gcgccacggt cgccggcccg
    22021 ctcctgcggc acgcgtgcga tggattcgat gcccctgatc tctcctcctc ggacggaact
    22081 atatgtcgta tgaaccagcc tctaggctgg cgaaagcttg agggtcgtgg gcagagtctc
    22141 gggacgaacc atcgcctcag tggaggatgg cgaagattgc tgatacttgc tcctgaaggc
    22201 ggcgcttacc gcgttcacag tcgcctcttc ggtggcgggg gctgccttca cggggatgcg
    22261 gtcgtcctcc acgtgcagcg cgccctccgg gttcgccgag atctcgcggt accagcggcc
    22321 tttggtgcca cgcaccgagc gcacgaagac ctcgccctcg acgaccacga cccagatgat
    22381 cgtgcggtgc tctggggcgt catcgtcccg gcgggtctcg atgcgcactt ccttggtctc
    22441 gtcgaggagc ctgagggtta cctcaccgaa agaatcgttc atggtttctc ctaagcgtag
    22501 cgttcatggt ggacgatctc accgaggggt ttacgcgcct gccggcggcc gccgcgggat
    22561 ctgcgggcct cctcggcggg gtagccgagg gagatcgcgg tcctcaccac tttctctctt
    22621 ggtacgccga gtatctctct ggccgcgtct ctgcccgggc cggcgaacca acctatgctc
    22681 gagccaacgc cgtgcgcgtg ggcggccagc atgatccgct ctgcgagctt cccctcatcg
    22741 taagtctcct gctcggcccg ctcgccggcc atgacgagca ctatcccgag cggcgcctcc
    22801 gccaggtgac ctgcgtagcc cccggccgag gcgagggagc gcaacgtctc acgctcgcgg
    22861 attatcagga tctcccaggg ctgtttgttg gtcgcgctac ccgaccagcg ggccacctcg
    22921 agaacgtcgt cgactacctc ctgtggtatt gggtcggatc tgaacgagcg gacggaccgc
    22981 aaggtgcgca ggaaggagat gcggtctttc gctgtccttt cgtttgctgt cgcgctcatg
    23041 gttccctcct ctcggcaaca ctcacatcga ttatccctag cttcacgtcc gctcctttca
    23101 tgtggttcat gctgctgtct ccgaggggga gagcaactgg gaaccgagga tctcgttgta
    23161 gagttcgctc tctcgcagga gctcctcgtg ggtgcctctg gccgcgatac ggccctcgtc
    23221 gaggaccagg atcaggtccg cgtcccgcac ggtggagacc ctctgggcga tgacgaagac
    23281 ggtgcggtgc ttctcgcgca tgagcctgtc caacgtctcc tggatcgcgg cctccgtctc
    23341 ggcgtccacg gccgaggtgc tatcgtccag gataaggagc ctagggtcga ttaggagcgc
    23401 ccgcgctatg gcgatccgtt gtcgctggcc acccgagagc ccgatgcccc gctcgccgat
    23461 aacggtgtcg tagccctccg agagccccct tacgaactta ccggcctggg cggcctcggc
    23521 cgccgccacg acctcctcgt cagtcgcgtc cggtttaccg aaggccacgt tatcgcggac
    23581 ggtgcccgag aagaggcgcg tctcctgcaa gactatcccg atctgggacc tgagcgagga
    23641 tagggtcaca tctcgcacgt cgttgccgtc gagcctgacg gcccctcccg tcacgtcgta
    23701 gaagcgcggt atgaggttga ctagagtgga cttccccgag cccgtagtcc ccaggatggc
    23761 gacggtctgt ccgggtcttg cggtaaagga cacgccggcc aggatgtcgc gcgcgctgcc
    23821 agggtaccga aacgagacgt tgtcgaaatc gaccctgcac gagagcggga ggagggtgga
    23881 ggcgtcgggg gcgtcttcta cgtcgagggg ggcgtcgagg acatcgaaga ccctctggga
    23941 cgaggctccc gcgcgggaga tgctcgccga gaggaagcct atcgtgagga tcgggaagag
    24001 catgaagccg agcagggtgt tgaaggcgac gagatccccg atggagagcc tgcccccgat
    24061 aacctgccag cccccaaacc agatgatcgc gagcgtcccc aggttggcga atagaaagac
    24121 gaaagggaag ttgttggaga ccacccgcac ggtggttagg ttcttctcca ggagctcttc
    24181 gttgaccgcc gtgtagcggg ctgtctcgta gtcctcgcgg gcgaaggccc ggatcacacg
    24241 cacccccaga aggtcttcct gcagcacggt gttgagcctt cccagcgtct gctggacttc
    24301 cctgaaaagg ggcctgatcc taccgacaaa gcggaccaag agtacagcga tgatgggtac
    24361 tacagccagc gcgacgaggg cgagttgcca gtcgagatag aggagcaaga tagtcgtgcc
    24421 tatgagcatg acgacggcgt tcgcgagttg gacgacaccg gagccggcga aggtgcggat
    24481 ctgctccacg tcgctggtca ggcgggtgac gagctggccc gtctggaccc tgtcgtagta
    24541 cgaaaaggag agccgctcta tcctctcgaa caaagcatcc cgcaagtcgt aagcgacgcc
    24601 ctgcgaggcc ctctcggcca gatatccctg caagaacgtg aacagggccc gtgccaacgc
    24661 gagcccgacc agccacccga ccacgacgaa gatgaccccg agccgccgcg gaccgatgcc
    24721 ttcgtcgatg gcgcgcccga tgagcagcgg cgtcgccagg ttagcccccg agaccaaaag
    24781 cagcgaaagg acagcgacca cgctctgcca cgtgtacgag cggaggtacg cgagcgccct
    24841 gcggagctcc cctgctcctg aaggcgtatt cactcaccct ccttcacgtt gcttccgact
    24901 tctgcgtcgt cgcggacatt atggtatccc atcgggacga acagatggtt ttctcggaag
    24961 gaacccggtc gctacggctt gggcggttcg cgaaccgctc ctacatgcga atcgctctaa
    25021 ttgaggggac cgatctcttg ttcggccgct tctacgagcg ctcctgaatt caccaggcgt
    25081 tctgccgctt cgaggtgcgg ggccatgaag gtatcgctct cgatgaacgg gatgtctcgc
    25141 ctgatccgct cgaggacggc gccggtcgca tggcccggtt gcaggggaga gcggaggtcg
    25201 agggcctggg ccgcgcaggc cgcttcgacg gcgaggatgc tggcgacgtt ggccaggagc
    25261 cgtcgcagtt tgcggcccgc gctccagccc atcgagttgt ggtcttcttg catggccgag
    25321 gtcgggaggg aacccgtgct cgccgggttc gcgaggcggc ggttctcttc cgcgagggat
    25381 gcggccgtgt agtgggggat catgaacccc gagttgacgc cggctcgcgg gcttaggaag
    25441 gctggcaggc cttccgaacg tctcgggtcg agcatgcggt cgatcctgcg ctccgtgatg
    25501 gcgcccacct ccgaagcggc gacggccagg aagtcgagga cgagggcgag cggctccccg
    25561 tggaagttgc cgacgctctc gacccggccg tcgggcagca cgaccgggtt gtccgttacg
    25621 gagtccaact cgcgcccggc tacgagggag gcgtgatcca gcgtgtcccg cgccgcgccg
    25681 aagacctggg gagcacagcg caatgaataa gcgtcctgga ccaggtgggg ggagtcacgg
    25741 tgggaggcca cgattgggct gcccgagagc agcttcgaga tgttgcccgc gctcgcgagt
    25801 tgtcccggat gggccctcag ggcgtggaga tcctcgcgga agaccctgtc ggtgccgagc
    25861 agggcctcga cggacatcgc cgcggtcaca tcggcggtct tcaggaggac cttgaggtct
    25921 tcgagggaga gcagcagcat agcgagcata ccgtccgtgc cgttgatcag ggcgagacct
    25981 tcctttgtgg cgagacctaa gggtcggatg ccggcgcgct ctagagcttc gctggtcttg
    26041 actggttctt ctccttccag cagccgacct tctccaacga ggcagagccc gacgtgggcg
    26101 aggggcgcga gatctccgc
//
LOCUS       DATMVF010000180         5079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_207219, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000180 DATMVF010000000
VERSION     DATMVF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207219"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..241
                     /locus_tag="VN178_08950"
     CDS             101..241
                     /locus_tag="VN178_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81139.1"
                     /translation="MGSVALGFDGFPSARAALSVGESPFKSAILVSIPHKLLRISEVL
                     VL"
     gene            310..1902
                     /locus_tag="VN178_08955"
     CDS             310..1902
                     /locus_tag="VN178_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWS81140.1"
                     /translation="MAESIDRGDASKTARLASPHEDLKDKGLEHDAVGLLASVVLGVS
                     CVAPAYALTATLGPTVSEVGLQMPAIFLAGFIPMLLVAYAYRELNHEAPDCGTSFTWT
                     VKAFGPHVGWMCGWGFVLATVIVLSNLVGVAVTFFYLLLGNVLGNPAIEALGGNAVVN
                     VLTTLVFVAAATAISYRGMAATKGVQYALVGLQMVVLALFVVLAFGKAVSGASDLAIS
                     FSWSWLNPFAIESFAAFAAGLSLSIFIYWGWDTTLTINEETTGSSSTPGRAALLTILT
                     ILSTYMLVAISAQMFAGIGEQGIGLGNPDTADNVFAALAEPVLGVLAIFLFLAVVASA
                     ASSLQTTFLPAARTMLAMSFYKAAPERFSHVHPRFRSPSYATLFSGVATAIFYTVMTF
                     LSEDVLIDTIYALGLMIAFYYSLTAFACVWYFRNELFTSPKNFIFKGLFPGLGGIMLT
                     LLFFKTAVDTFDPSYGSGGAVLGMGTVFVIGIGLLLLGVILMFAWQLRAPEFFRGETL
                     THDTAVLATEEDLLQDRDPEEN"
     gene            1904..3844
                     /locus_tag="VN178_08960"
     CDS             1904..3844
                     /locus_tag="VN178_08960"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HWS81141.1"
                     /translation="MGQPTVETESPAAARHPLDPLNADEFRRAANTLREAKGFDERWR
                     FAAIELKEPSKEVLASGERVPREALLVCWNREDGQAYKAVVSLDEDRLVSWEHRPGEQ
                     PNVTVDEYHECDEALRRDPQVVEALAARGVTDMEKVLFDVWAYGGSLIADTYRGRRIG
                     WTDIWYRDHEDSNPYANPVNGLKLIVDLNRAELLEIEDTFAVEKPKIMGEYVPKHVPG
                     LQERGDLKPLEITQPEGASFVLAGNELRWQKWFMRVGFNYREGLVLHTVGYDDGGKIR
                     PVAHRMSFSEMVVPYRDPSPEHFRRTAFDVGEWGLGFMTTSLELGCDCLGEISYLDAV
                     LHDSSGEPYAIKNAICIHEEDDAVLWKHVDEIAGAEVRRSRRLVLSFHATVANYEYLV
                     YWRFYQDGNIECEVRATGIMVTSHFPEGEQPPYGSLVDDRTYAPFHQHFIVARLDLDV
                     DGEANTVVMSETEASPVGPENPYGLALVQRNTPLRTEEEGKQDYDWNTQRSWKVVNDN
                     AENGLGTSTAYKLVPTGCLPAMVDPSSPVLERAGVIGHTLWVTPYHQDERWPCGEFVN
                     QSEADTGLPVWTRKNRPIENTDVVLWYVFGIHHVTRPEEWPVMAADTVSFWLKPAGFF
                     DRNPALDVPPTANGHCHNPEGG"
     gene            3849..4844
                     /gene="speB"
                     /locus_tag="VN178_08965"
     CDS             3849..4844
                     /gene="speB"
                     /locus_tag="VN178_08965"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWS81142.1"
                     /translation="MIDPQLRWASIGEKPDYAGPLSYAGMPLTQDPVDLDGVDVAIVG
                     APTDDLVSDRPGTRFAPRAIRAASCPPGPQLETGVDAMSELRVVDFGDAPVLPADPVR
                     SHAAIESTVGQVLAAGPIPVVLGGDHSISEPNVRACATAHGPLGLVHFDTHTDTGAEV
                     FGVELSHGTIMRRLVEDGHVDPLRYAQIGLRGYWPGDEEFSWQAERGITSLFMHDVRD
                     LGIKEVIRRALEAIGPGPAFLSVDIDVLDPAFAPGTGTPEPGGMSTAELLWAAREVAS
                     GVELVGADLVEVIPTGVGGADITALAADRIVREILGGIALRRRRQPDGQTTKEER"
     gene            4841..>5079
                     /locus_tag="VN178_08970"
     CDS             4841..>5079
                     /locus_tag="VN178_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWS81143.1"
                     /translation="MTETRTLKNFVGGEYVDASNGRTYDLVNPATGQTFAQAPISEQE
                     DIDRAFQAAQRAFETWRDTTPSERQLALLRLADAI"
BASE COUNT          912 a         1653 c         1653 g          861 t
ORIGIN      
        1 cacatgggat tcttcggacg cacccccgag accgtcgacc ccgaggtcgc cgaaggtcgg
       61 gtgaaggtgc aggagaccgc cggcgccgga ggagggttaa atgggaagcg tggcgctcgg
      121 cttcgacggc ttcccttcgg ccagggcggc tctgagcgtg ggcgagagtc cgttcaagag
      181 cgccatcctg gtctcgatcc cgcacaagct cctgcgcatc tcggaggtac tcgtgctgtg
      241 agtagcgaac gttcaccata ggcgagtaaa atggagcgta cgggcgggct ccttgggaaa
      301 ggagacaaca tggcagagtc gatcgatcga ggcgacgcct cgaagacggc cagattggct
      361 agtccacacg aagacctcaa ggacaagggt cttgagcatg acgccgttgg acttctggcc
      421 agcgtcgtac tgggtgtctc ctgcgtggct ccggcctatg ccctcaccgc caccctcggc
      481 cccacggtca gcgaggtcgg tctgcagatg ccggcgatct tcctggcagg gtttataccg
      541 atgttgctcg tggcctatgc ttaccgggag ctcaatcatg aggcgccgga ctgcggcacc
      601 tcgttcacgt ggacggtgaa ggccttcggc ccgcacgtcg gctggatgtg cgggtggggc
      661 ttcgtgctgg caacggttat cgtgctctcc aacctcgtgg gcgtggcggt cacgttcttc
      721 tacctgctgc tcgggaacgt cttggggaac ccggccatcg aggcgctggg cggcaacgcg
      781 gtcgtcaacg tcctgactac cctggtcttc gtggcggccg ccacggcgat ctcctaccgt
      841 ggcatggcgg caaccaaagg ggtccagtac gcgctcgtgg ggcttcagat ggtcgtcctc
      901 gccctcttcg tcgtgctggc cttcggcaaa gcggttagtg gggcatccga tctcgccatc
      961 agcttttcgt ggagctggct caaccccttc gcgatagagt ccttcgcggc tttcgcggcc
     1021 gggctgtcac tctctatctt catctactgg ggctgggaca ccacgctcac catcaacgag
     1081 gagactaccg gcagctccag tacgccggga cgagccgcgc tcttgaccat tctcacgatc
     1141 ctcagcacgt acatgctggt tgcgatctcg gcccagatgt ttgccggcat cggcgagcag
     1201 ggcatcggcc tgggcaatcc cgacaccgcg gacaacgttt tcgccgcgct cgctgagccc
     1261 gtgctcggag tgctcgccat ctttcttttc ctagcggtag tggccagcgc ggcgtcgagc
     1321 ctgcagacca cgttcttgcc cgcggcgcgg acgatgctcg cgatgtcctt ctacaaggcg
     1381 gcgccggagc gtttctcgca cgtgcacccg cgatttcggt cgccgtccta cgcaacgctt
     1441 ttctccggcg tggccacggc gatcttctac accgtaatga cattcctaag cgaggacgtc
     1501 ctgatcgaca ccatctacgc gctcgggttg atgatcgcct tctattattc gctcacggcc
     1561 tttgcgtgcg tgtggtactt tcgcaatgag ctgttcacga gcccgaagaa cttcatcttc
     1621 aagggcctct tcccgggtct cggcgggata atgctcacgc tcctcttctt caaaacggcc
     1681 gtcgatacct tcgacccgtc ctacggcagt ggcggagcgg tattggggat gggcaccgta
     1741 tttgtaatcg ggataggcct gctgctgctg ggcgtcatct tgatgttcgc gtggcagctc
     1801 cgggcaccgg agttcttccg cggagagacc ctcacacacg acacggccgt gctcgccacc
     1861 gaagaggatc tgctgcagga cagggacccg gaggagaact gagatggggc aacccacggt
     1921 cgaaacggag agtcccgcgg cggctcgaca tcctctcgac ccgctgaacg cggacgagtt
     1981 tcgccgggcg gcgaacaccc tgcgcgaggc caaaggcttc gacgagcggt ggcgcttcgc
     2041 cgccatcgag ctcaaagagc cgtccaaaga ggtgctcgcc tcaggggaga gggtaccgcg
     2101 ggaggccctg ctcgtatgct ggaaccgcga ggacggccag gcctacaagg cggtcgtctc
     2161 gctggacgag gaccgactcg tctcgtggga gcaccgcccc ggcgagcagc cgaatgtaac
     2221 cgtcgacgaa taccacgagt gcgacgaggc gctgaggcgg gatccacagg tcgtcgaggc
     2281 gctcgccgcg cgcggcgtca ccgacatgga gaaggtgctc ttcgacgtct gggcctacgg
     2341 ggggtctttg atcgccgaca cgtaccgggg ccgtcgcatc ggctggacgg acatctggta
     2401 cagggaccac gaggactcca acccctacgc caaccccgtc aacggcctca agctaatcgt
     2461 cgacctcaac cgcgcggagc tgctggagat cgaggacacc ttcgcggtcg agaagcccaa
     2521 gatcatgggc gagtacgttc ccaaacacgt ccctggccta caggagcgcg gcgatctgaa
     2581 acccctcgag atcacgcagc ccgagggggc ctccttcgta ctggccggca acgagctcag
     2641 gtggcagaag tggttcatgc gcgtcggttt caactaccgc gaggggctcg tgctccacac
     2701 cgtaggttac gacgacggcg gaaagatcag gcccgtggcg caccggatgt cgttctcgga
     2761 gatggtcgtg ccctacaggg atcccagccc cgaacacttc cgacgcacgg cttttgatgt
     2821 cggcgagtgg ggcctcgggt tcatgactac ctcgctggag ctaggctgcg actgcctggg
     2881 cgagatctcc tacctcgacg cggtactcca cgactctagc ggggagccct acgccatcaa
     2941 gaacgccatc tgcatccacg aggaggacga cgcggtcctc tggaagcacg tggacgagat
     3001 cgccggtgcg gaggtgcgca ggagccgccg gctggtcctc tccttccacg cgaccgtagc
     3061 gaactacgag tacctcgtgt actggcgctt ctaccaggac ggcaacatcg agtgcgaggt
     3121 gcgggccacc ggcatcatgg tcacctccca cttccccgaa ggcgaacagc ccccgtacgg
     3181 gtctctggtc gacgatcgca cctacgcccc gttccaccag cacttcatcg tcgccaggct
     3241 cgatctcgac gtggacggcg aggcgaacac ggtcgtcatg tccgagaccg aagcgtcgcc
     3301 ggtcggaccc gaaaacccct acggtctcgc cctcgtgcag aggaacacgc ccctgcgcac
     3361 cgaagaggag ggcaagcagg actacgactg gaacacccag cgctcctgga aggtcgtaaa
     3421 cgacaacgcc gaaaacgggc tgggcacgtc caccgcctac aagctcgtac ccaccggctg
     3481 cctgccggcg atggtcgatc cctcctcgcc ggtcctcgag cgggccggag tgatcgggca
     3541 cacgctatgg gtcaccccct accaccagga cgagcgctgg ccctgcggcg agttcgtcaa
     3601 ccagagcgaa gccgacacgg gactaccggt atggacccga aagaaccgtc ccatagagaa
     3661 caccgacgtg gtgctctggt acgtgttcgg catccaccac gtaacgcggc ccgaggagtg
     3721 gcccgtgatg gccgcggaca ctgtctcgtt ctggctcaag cctgccgggt tcttcgaccg
     3781 caacccggcg ctggacgtgc cgcccacggc taacgggcac tgccacaatc cggaaggcgg
     3841 ataggagcgt gatagatcca caactacgct gggcatccat cggcgagaag ccagactacg
     3901 caggaccgct gtcctacgcc ggcatgcccc tcacccagga tccggtagat ctcgacggtg
     3961 tcgacgtggc tatcgtgggc gcgcccacag acgacctagt ctcggaccgg cccggaacgc
     4021 gcttcgcacc gcgcgccatc cgggccgcga gctgcccgcc cggaccgcag ctcgaaaccg
     4081 gggtagacgc gatgtccgag ctgcgggtcg tggacttcgg ggacgcaccc gttctgccgg
     4141 cggaccccgt ccgctcccac gccgcgatag agtcgaccgt ggggcaagtg ctcgccgcgg
     4201 gcccgatacc ggtggtcctc ggcggggacc actcgatctc cgagccgaac gtgcgcgcct
     4261 gcgccaccgc gcacgggcca ctgggcctcg tccacttcga cacccacacg gacaccggcg
     4321 cggaggtctt cggcgtcgag ctctcccacg gcaccataat gcgccggctc gtggaggacg
     4381 ggcacgtcga cccgctgcgt tacgcccaga tcggtctgcg gggctactgg ccaggggatg
     4441 aagagttctc ctggcaggcg gagcgcggca taaccagcct cttcatgcac gacgtccgag
     4501 atctggggat aaaggaggtg atccggcgtg cgcttgaggc catagggccg ggtcccgcgt
     4561 ttctgagtgt ggacatagac gtgctcgatc cggccttcgc gccaggcaca gggacgcccg
     4621 agccgggcgg gatgagcacc gcggagctgt tgtgggcggc gcgggaggtg gcgagtggcg
     4681 tcgagctggt aggggctgac ctggtagagg tgatcccgac cggtgtcgga ggagcggaca
     4741 taacggcgct cgcggcggac cggatcgtac gcgagatcct cggcggcatc gccctccgcc
     4801 ggagaagaca acctgacgga caaacgacca aggaggagag atgacggaga cacgcacgct
     4861 gaagaacttc gtgggaggcg agtacgtgga tgcgtccaac ggccgcacct acgatctggt
     4921 caacccggcc acgggccaga ccttcgccca ggcaccgatc tcggagcagg aggatatcga
     4981 ccgggcgttc caggcggcac agagagcctt cgagacgtgg cgcgatacga ccccttcaga
     5041 gaggcagctc gcgctcttga ggctggccga cgcgataga
//
LOCUS       DATMVF010000181         4864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_302206, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000181 DATMVF010000000
VERSION     DATMVF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302206"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1504
                     /locus_tag="VN178_08975"
     CDS             254..1504
                     /locus_tag="VN178_08975"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS81144.1"
                     /translation="MARVSEGDHVLRSIADWDLARAHWMRGELAEAERAMRGELAKAV
                     GAVRAGRRDPYLVLVAWWDLGRVRSAQGTLRSALRTYRDALELVAEAGDSSAPAGIAH
                     VGLAEILREQNELDVALEHTDEGGGHCRSLPNRMLLAAGLTILARIRQALGDTSGTLE
                     AIGEAEQVGLSPEITDLFNPVPRQRVRLMLIQGEIVEAARWTAERVLGVEDEVSYLRE
                     PEHLVLARVLLAGEASKPEQARLLLERLIVAAEAAKRTGSVIEILSLLASAMWAKNDK
                     EQAVNILARAVALAEPEGYVRTFVDEGAAMGDLLSEVLNARQRGSLDPPVPAHYLRKL
                     LAATEKDATGAVPPAVGLSEPLTERELEVFVLIAAGKSNRRIATELYVGMGTVKTHIR
                     NIYRKLEAHSRTQALARARELNLI"
     gene            1707..2171
                     /locus_tag="VN178_08980"
     CDS             1707..2171
                     /locus_tag="VN178_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWS81145.1"
                     /translation="MTPAYPDLFKEDDVMEEGKISRRRFLGLGAGLGLAAGAAPLLGA
                     CGAEDCPEVGEGQVIADKADLAPGSAFPFTDAETGDPGVLVNLKSGEFVAYSAVCKHR
                     GCTVAYEDSKLACPCHGSVYDPANGAEVVAGPASQPLDEIPVEVRGGEVLCA"
     gene            2340..2462
                     /locus_tag="VN178_08985"
     CDS             2340..2462
                     /locus_tag="VN178_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81146.1"
                     /translation="MRKIVASEFVSLDGVLEEGGVIDGRFGDSAVPKEAVWGSY"
     gene            2447..3130
                     /locus_tag="VN178_08990"
     CDS             2447..3130
                     /locus_tag="VN178_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS81147.1"
                     /translation="MGFVLKNERPADSPFVETVWRSRSGPAGVFTSVAASNWEMVLVT
                     YGGETTVTARGPETRPSEAEYPADAEYLGITFELGTFMPHLPIKALLDRQDMILPEAS
                     SSSFWLHGSAWELPTFENAEAFVERLVRREILVRDPVVEAAIRGHAPEVSVRSLQYRF
                     LRATGLTQKTIRQIERARFAVSLLERGAPIPDAAFGLGYFDQAHLTNSLKRFVGRTPA
                     QIARKSAVG"
     gene            3230..3568
                     /locus_tag="VN178_08995"
     CDS             3230..3568
                     /locus_tag="VN178_08995"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWS81148.1"
                     /translation="MQLALCYYYDPATDGPTEGEIPEWMDFAAKVKDAGVLVYEAGFH
                     SAQTAKTLSARDGRVTTEDGGVVKSGDVLAGLVVVDVPDVDVGLDWARQIPTARYGKV
                     EVRPVVEWEG"
     gene            3587..4120
                     /locus_tag="VN178_09000"
     CDS             3587..4120
                     /locus_tag="VN178_09000"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HWS81149.1"
                     /translation="MSTSKFAAPKAGIRSGSNTRTLLGALALSSPLWTVVSLAQAATR
                     EGFDLARHPLSMLSVGSLGWLQITNFIVAGVLAILGAVGLKQAIPSRWAPRLVIIYGI
                     GYILSGVFVMQPGDGFPAGTPLGQSGPLAWHTVVHLFVGMIAFIALTAVLFVLGRYFA
                     LHGERGWAWGPAPPPSE"
BASE COUNT          886 a         1532 c         1637 g          809 t
ORIGIN      
        1 ccgggatcag cgggaagtct gctaccagcg cgcataggaa gtagaatggg tggtggaggt
       61 gagcagaaga tgccccttac ggccgattcg ctcgtgtcta ccaagctgcg ccccctggag
      121 gcgcgtccca agctggtcgc gcggccgcgt ctgaccgaga agctggagcg ggagtcggga
      181 cgcaggctca cgctcatctc tgcccccgcg ggtttcggca agagcaccct gctcggagag
      241 tggctctcgc gctatagccc gcgtgtcaga gggggatcac gtcctgcgca gcattgccga
      301 ctgggacctg gccagggcgc actggatgcg cggcgaactg gccgaggccg agcgagcgat
      361 gcgcggtgag cttgccaaag ccgtgggagc ggtacgcgca ggacgacgtg atccctacct
      421 cgtcctggtc gcgtggtggg acctcggcag ggtgcgcagc gcccagggca cgttacgctc
      481 ggcgttgcgc acctaccggg atgccctgga actcgtcgcc gaggcgggag actcgtcagc
      541 gccggcgggc atcgcgcacg ttggccttgc cgaaatcctg cgcgagcaga acgagttgga
      601 tgtcgccctc gaacataccg acgagggcgg cgggcactgc agatcgctac ccaatcgcat
      661 gctactcgcc gcgggcctca ccattctcgc ccgcatccgg caggcgctcg gcgatacgtc
      721 tggcaccctg gaggcgatcg gtgaggccga gcaggtcggg ctgagcccgg agataaccga
      781 tctcttcaac cccgtgccga ggcagcgagt gaggctgatg ctcatccaag gagagatcgt
      841 cgaagccgcc cggtggaccg ccgagcgcgt gctcggcgtg gaggacgagg tcagctacct
      901 gcgggagccc gagcacctcg tcctggcgcg cgtgctgctc gccggcgaag ccagcaaacc
      961 cgagcaggcg cgcctactgc tcgagcgcct gatcgtggcc gcggaggcag cgaagcgtac
     1021 gggcagcgtg atagagatcc tgtcgctcct ggcttcggcg atgtgggcca agaacgacaa
     1081 agagcaggcc gtgaacatcc tggcgcgggc agtggccctg gcagagccgg agggctacgt
     1141 caggaccttc gtcgacgagg gagccgcgat gggagatctc ctatccgagg tactcaacgc
     1201 ccggcagaga ggaagcctcg acccgcccgt cccggctcac tatctcagga agctactggc
     1261 ggcgaccgaa aaggacgcga cgggcgcggt gccgccggcc gtggggctgt ccgaaccctt
     1321 gaccgagcga gagctcgagg tgttcgtcct catagcagcc ggcaagagca atcgccggat
     1381 cgccacagaa ctctacgtcg gcatgggcac ggtcaagacc cacatccgca acatctaccg
     1441 caagctagag gcgcacagcc gcacgcaggc cctggcgcga gccagggaac taaacctgat
     1501 ctagcaaccc gtacgcagag ttccgaaacg ccctactgcg acgctcgtcc acgccgggga
     1561 ggtcggaggg tttcgaatcg gcgcgcgtgt ggccgtggtg attgagggca caataatctc
     1621 tacctccctt ctagtccttt aggtggatgc ctctccaccc ggactcgcaa taccatcgaa
     1681 cgcaaaccca aagaaggaga tgaaccgtga ccccagctta ccccgacctc tttaaggagg
     1741 atgatgtgat ggaagaaggg aagatctcga ggagacggtt cctggggctt ggcgccggtc
     1801 tcgggctggc cgcgggagcg gcgcctctcc tgggcgcctg tggtgccgag gattgtccgg
     1861 aggtcggcga gggacaggtc atagccgaca aagccgacct tgcgccgggc tcggccttcc
     1921 ccttcaccga cgccgaaacg ggggatcccg gtgtgcttgt aaacctgaag agcggagagt
     1981 tcgtggccta ctcggcggtc tgcaagcacc ggggttgcac cgtcgcctac gaggacagca
     2041 agctcgcctg cccgtgccac ggctccgtct acgatcccgc caacggagcc gaggtagtgg
     2101 ccggtcccgc gtcgcaaccg ctggacgaga tcccggtcga ggtccgcggc ggagaggtgc
     2161 tttgtgctta ggaagacttc gggcatcagc aagaacccga agcctgagtc ctgaaggtac
     2221 ggatcgaggt caatgacggg actcagccag ggcggacaag cgcggaggct tgaattcgag
     2281 caaatgcgat tgatcgtagt aagtcaacga ccactatcta cgagatcgga ggaggtcgga
     2341 tgcgaaaaat agtagcgtcg gagttcgtgt cgctggacgg tgtcctcgag gagggcggcg
     2401 tcatcgacgg tcgattcggc gattctgctg ttcccaagga ggcggtatgg ggttcgtact
     2461 gaaaaacgag aggccggcgg actcgccttt cgtcgagacg gtctggcgct cccgcagcgg
     2521 gcccgccggt gtcttcacct cagtggccgc cagcaactgg gagatggtgc tcgtgaccta
     2581 cggcggcgag acgacggtca ccgcgcgggg ccctgagacc aggccgtcgg aggccgaata
     2641 ccccgccgac gccgagtacc tcggcatcac cttcgagctc ggcacgttca tgccccatct
     2701 ccccatcaag gccctactgg acaggcagga catgatcctt cccgaagcgt cgagcagctc
     2761 cttctggctg cacggctccg cgtgggagct tccgaccttc gagaacgccg aggcgttcgt
     2821 cgagaggctc gtccgtcggg agatactggt gcgcgatccg gtggtcgagg cggcgatccg
     2881 gggacatgcg cccgaggtgt cggtgcgttc cctgcagtac cgtttcctgc gggcgaccgg
     2941 gttgacccaa aagacgatcc ggcagatcga acgcgcccgc ttcgccgtct cgctgctcga
     3001 gcggggcgcg cccatcccgg atgccgcctt cggactcggc tacttcgacc aggcacactt
     3061 gaccaactcg ctcaagcgct tcgtcggaag gacgccagcg cagatcgccc gcaaaagcgc
     3121 cgtcggatag tttgcgcttt tttccaaggc tttctccttt tgccggtcta ggattacggt
     3181 gtagacgaga aacgggccgg atgcccccac ttcaggaagg agcaacgcca tgcaactagc
     3241 gctgtgttac tactacgatc ccgccacgga cggccccacc gaaggggaaa tccccgagtg
     3301 gatggacttc gccgcgaagg tcaaggacgc gggcgtgctc gtgtacgagg cggggttcca
     3361 ctccgcgcag acggcgaaga cgctcagcgc gcgtgacggt cgggtcacca ccgaagacgg
     3421 cggcgtggtg aagtccggcg acgtcctggc cggcctggtc gtcgtcgacg tcccggacgt
     3481 ggacgtcggc ctcgattggg cgcggcagat tccaaccgcc aggtacggca aggtcgaggt
     3541 acgcccggtc gtcgagtggg aaggttgaag gcgaaaggta aggacgatgt ccacttcgaa
     3601 gtttgcagca cccaaggccg gcatccgctc cggcagcaac acccgcacgc tactcggcgc
     3661 gctcgccctg tcgagcccgc tctggacggt cgtgtctctt gcgcaggccg ccacccgtga
     3721 gggcttcgac ctcgcccgtc acccgctcag catgctctcg gtcggatcgc tcggctggct
     3781 tcagatcacg aacttcatcg tcgccggagt gctcgcgatt ctcggtgccg tcggcctgaa
     3841 gcaggcgatc ccgagcaggt gggcgccgcg actggtgatc atctacggca tcggctacat
     3901 cctctctggc gtcttcgtca tgcagccggg cgacgggttc cccgccggca caccgctggg
     3961 acagtccggt cccctcgcct ggcacactgt ggtacatctc ttcgtgggga tgatcgcgtt
     4021 catcgcgctc acggccgtcc tgtttgtgct cggccgctac ttcgcactcc acggcgagcg
     4081 aggatgggcc tgggggcccg cgccgccgcc atcggagtga tagtcgcgaa cgtgttgtcc
     4141 gcggcacagg ttctcgcacc gtccgtcgtg ctggcggttg gagtcatcgt gggcatgctg
     4201 tttctgtcgc tcatcgccgt gaagctgcga cgagacgtct gaccggcggc caagggccgg
     4261 gatcgtcccc gtgctcttgg ccgtcctggc catcctcgta ccccgcctcg gcggcgacga
     4321 actagcccat cccctcgaca atccagcttg gcagaagccc agcggcgtgc atgacaggcg
     4381 aacggaccgg agtccagcaa ccccggccct ccttcctggt tcgagaccct tcgtgccgag
     4441 ggatctctac cccttcgatc tacctttcgg gtgatgccgc tctacccggt ttcgcaatat
     4501 catcgaagcc aattcaatgg aaggagacga gatgcagagt ttggcgacaa ggtgaacacg
     4561 atgaggctgg cattcaccac gttgcccgaa tggagacaga atggctggtg tgaggagaac
     4621 cacctaccgc gccggagcag acaaggaatg ggcagcctga cagatacgcg atttcgggtc
     4681 gtgaagtagc caccgagggg cggtgttcga ggtgcgtagc cgtccgcccg gcttccggta
     4741 ggcggcggat cggagaggag cacgatgacc caccaacgag agcagatgga gacaagaaac
     4801 gggcaggaga agaaggcgac gctggttttg ggcgtcagag gcaagaccgg ccgccgggta
     4861 gtgc
//
LOCUS       DATMVF010000182        11815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_525911, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000182 DATMVF010000000
VERSION     DATMVF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525911"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VN178_09005"
     CDS             <1..902
                     /locus_tag="VN178_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWS81150.1"
                     /translation="QLVEAVDGFGEHDIDAWLWGEWHGDRAVGDKTTERPQTLGVVGK
                     LQRRRHLSVEEFVYARGRTERTVKVTLPSPSMYANLWSPELSAGVYPTLDLFLEDVAR
                     ILREEVEELVRLGAEYIQLDAPHYPLLLDPKTRAFYEGLGWDLDRWLSRGIELDNYVI
                     GDHDDVTFSFHLCRGNQGSRWLVSGSYEPLARRIFGGINAQRLMLEYDDERSGDFAPL
                     SHVPEDKVAVLGLVTTKTPRRETKEELEARLHEAARFIPLERLALSPQCGFATSIIGN
                     AISVEDEEYKLRTIVETAEQVWG"
     gene            complement(916..1824)
                     /locus_tag="VN178_09010"
     CDS             complement(916..1824)
                     /locus_tag="VN178_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81151.1"
                     /translation="MRVLVTGARGKVGRATVAALQVAGHDVRATDLTPPVFERPEEGE
                     ADYVQADLSDAGDAFAVVRGAEAVVHTAAIPEPTSNPPHVVFQNNLMATFNTLEAAIR
                     FGVTRFVNTSSETVPGFFFPERPFLPDYVPVDEEHPIRPQDPYATAKYFGELLMDGAI
                     RRSDIRCISIRPSWVQHEGNYERNLGPMLRDRSELSAGFWSYIDVYDLADAMVLAAES
                     DLPDHEVFYIASPDNVGGRPFEAAVREHYGEQVEVKTPLSREDSSGISIEKARRMLGY
                     DPKRSWRDYLDEDGRLRSNVKGLFTG"
     gene            1911..2966
                     /locus_tag="VN178_09015"
     CDS             1911..2966
                     /locus_tag="VN178_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWS81152.1"
                     /translation="MRVVVVGATGNIGTSVLRSLENEESVESVLGLARRMPDLTVPKV
                     EWASADVVSEDLVPHFRGADAVVLLAWLIQPSRNLTKQWMVNVEGSTRVARAAKEAGV
                     PSLLYASSVGAYSAGPKDRRVDESWPTGGVPSSYYGRHKAEVERRLDRFEAEAPKVRV
                     VRMRPGLVFKKEAAEGIRRLFAGPFFPSVLANPALINVIPDIENLRSQVIHSYDVGEA
                     FRLGIVNGDVRGPFNLAAEPVLDAGEIGRILNARPVPTSAGVVRAGVGLSWALRLQPV
                     PPGWLDLSRNVPVMDTTKARTELGWIPRYSADEALLDLLEGLRTGSGLDTPPLSPKTG
                     GRFRIREMLTGIGGREP"
     gene            2963..4135
                     /locus_tag="VN178_09020"
     CDS             2963..4135
                     /locus_tag="VN178_09020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HWS81153.1"
                     /translation="MKAVVWHGKEDVRVDNVPDPIIQEPTDAIIRITSTAICGSDLHL
                     YAKLSPLMREGDIIGHEPMGIVEEVGSDVEHISPGDRVVVPFNISCGHCYFCDQQLFS
                     QCENTRDEGKLARSVNLLGRGRGASLFGYTHVYGAVPGGQAEYLRVPQAHFGPIKVPE
                     GPPDERFLYLSDVLPTSWQAVAYADIPEGGTVGIWGLGPIGQMCARIAMHQGAGRIIG
                     IDNVPERLAMASRHGVQTVDYSAVDSVRDVILNLTDGRGVDSAIDAVGMEAGGSLVGS
                     ILQSTKLQLDRATALRDSMSAVRRGGTLSLTGVYAGPIQAFPLGDLFDMQIQLRMGQA
                     NVWQWVEDILPLLTGDDDPLGVEDFKTHEMPLEDAPLGYEMFQKKQDGAIKIVLKP"
     gene            4382..4486
                     /locus_tag="VN178_09025"
     CDS             4382..4486
                     /locus_tag="VN178_09025"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HWS81154.1"
                     /translation="MITLNHSQALFSLMDAAYGGDGRTTFALPILPGA"
     gene            complement(4512..4940)
                     /locus_tag="VN178_09030"
     CDS             complement(4512..4940)
                     /locus_tag="VN178_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWS81155.1"
                     /translation="MILYLDTSALVKVFVSEGGRELVVRAVKESSKIATSMVSYVEAR
                     ATFARLLREEALTEEEHTGVVAALDHRWPSYERPPITENLIRLAGDLAQRYALRGYDS
                     IQLASALVCHGRQRDLRFLSFDDDLNEAAKQIVLVYEVSS"
     gene            complement(4937..5185)
                     /locus_tag="VN178_09035"
     CDS             complement(4937..5185)
                     /locus_tag="VN178_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81156.1"
                     /translation="MRRATVTIPDGLEKALDSYARDLEFPPSLAAVMQAALREYLSRR
                     GYPASGEPVAGERPRVYEDAPTLRGNKTAAEMVIEDRR"
     gene            complement(5307..6326)
                     /locus_tag="VN178_09040"
     CDS             complement(5307..6326)
                     /locus_tag="VN178_09040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWS81157.1"
                     /translation="MKLDAGLGTEGDYLKGIDRTARAAEDFGFSGLWTSETKHDAFLP
                     LAIAANETHEIELGTSVAIAFSRSPMETAQTAWDLQNLSDGRFVLGLGTQVKAHVTRR
                     LSMPWGKPAARLREYIMALREIWDSFQNEGALKFEGEFYRHTLMTPFFNPGPIDHPQI
                     PVYIAGVNTRLARLAGEICDGFHVHPFHSPEYVRRTVIPAITAGAEGAGRDPGQVTLA
                     TSAFVISAESEVEAAEQRESVRAQISFYASTPTYRTVLEAHGWEEVGEKLGTMAREKK
                     WREMPALVTDEMLAAFAVEAAPDDIGSALEERYTGLIDRVTLYIPFVPGERDAFWQTV
                     VESVR"
     gene            complement(6336..7307)
                     /locus_tag="VN178_09045"
     CDS             complement(6336..7307)
                     /locus_tag="VN178_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWS81158.1"
                     /translation="MKAIRITEFGGPEVLGYEDVETPEPGAGQARVKLAASGVNFIDV
                     YQRTGLYPGDLPRTLGLEGAGEVDAVGEDAGDVSPGDFVAFASTPGCYAEYVVAPAEN
                     LVPFNPTLVEARVAAAVMLQGMTAHYLTHSTFPLQEGQTALVHAAAGGVGLLLVQIAK
                     LRGATVIGTTGTEEKAKLAREAGADEVILYTEQDFVEETNRITGGEGVHVVYDSVGKA
                     TFDGSLDVLRPRGYMVLFGASSGPVPPFDPQTLNQKGGLYLTRPALAQYTATREELLW
                     RAESLFSWIGQNNLNVRIGGTYSLEDVEQAHRDLEGRKTTGKLLLIP"
     gene            complement(7502..9109)
                     /gene="pknB"
                     /locus_tag="VN178_09050"
     CDS             complement(7502..9109)
                     /gene="pknB"
                     /locus_tag="VN178_09050"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HWS81159.1"
                     /translation="MSETRRTVLDDRYDVVRPLGSGGMGEVYLARDRVLGRDVALKVL
                     RRQYAGDDEFAERFKREAMSAASLSHPNIVQVYDRGETEEGASYIAMEYVPGGTLKER
                     ISREGPLEAADAAGVVAQVAGALGAAHDRGMVHRDVKPQNVLLTARGGAKVADFGIAR
                     AGSSATISRTGSVLGTAGYMSPEQALGKPATPKSDLYSLGVVLYEALTGELPYTADNP
                     IAVSMKHVNEPLRPPIELNPRIPRGMNAIVTKLLAKDPEDRYADADVLAEDLWRVNRG
                     LKPVSAGVLRKREQITQRNAPASQNVTNKMHRPRPGPVAYRRRRRLPWLLIALAAILF
                     TLGTLGGVQAVLGPDATAGWFDVFQGEQRPPGQPAGQVEVPEVDGLPRSLAQKRLTDA
                     GLAVGQVSSFPSDSVAAGRVIEPGIAAGTPVDPGTDVNLTISSGAQPSSSASSSASAQ
                     SSPEPGASASATSSASARPTPRENRQAAGTNGPEANGPNGAKPKVKKSPSAQKQNSAA
                     PTKEPADNSGGQGRGRGRGRSGESGED"
     gene            9314..9832
                     /locus_tag="VN178_09055"
     CDS             9314..9832
                     /locus_tag="VN178_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81160.1"
                     /translation="MATSGVEVDAMRLLGAFYDLSEGRLTEPVPIGDPEPGAAPRADL
                     DPDTPECDIAIRYLVDQRFIAPAHAASEYTITVAGFDRVREMRGIGGPEAPAGRNRLS
                     DKTQKQLMTLLSIGISIGLSQPLARFIDKNIPERRGIKDDLTEAVLQGLVRVVALVVA
                     SVIVRRLAASRR"
     gene            9934..10839
                     /gene="rbsK"
                     /locus_tag="VN178_09060"
     CDS             9934..10839
                     /gene="rbsK"
                     /locus_tag="VN178_09060"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HWS81161.1"
                     /translation="MADVFVAGSINQDFVLSVQRRPAPGETVTDARLATHNGGKGANQ
                     AAAAALLGASVAFLGRVGDDGFGEPLVRALADKGVDTGLMQEVPGSSTGAAFITVTPD
                     GENAITVAPGANRRLTAEDVDEASESIGDAKVLVAQMEIPPEVVLRAAEVAAASGTRV
                     LVNLAPPFEVPRTLLEKLDPLVINEHEAAFLLGERVEGVDEALDAAPGLLALGSGSVV
                     VTLGEDGAVFSDGESAQHLTAPKVEVVDTTGAGDAFVGALAARLAADAPLKDAVAYAV
                     RAGAAAVTEAGAQGALPSPDVVEAL"
     gene            complement(10844..11671)
                     /locus_tag="VN178_09065"
     CDS             complement(10844..11671)
                     /locus_tag="VN178_09065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS81162.1"
                     /translation="MISKELLSIVRPCPSDTEGLLYAGGIESDGMILGGKARSPATGE
                     TYEVKDGYLDLLKSRTGADSFANLTNFLPGAGRGYEPLWRVRSLSLLTGESFPNEREL
                     EIIHRLVRPVRSGRYLDLGCSAGLYSRSLSANLDSGIAVGIDISPSMLREAVRRAHAV
                     EPKPSFIRATAKQLPFADTSFTGAVCGGTLNEFGDPARVLRETSRVLEPGGRLAIMGI
                     LRVATPRGRRLQRLLSTGGIRFFDPDELHSLLDHAGFEPDPPQTYGPVFFAGSTRRG"
BASE COUNT         2023 a         3689 c         3924 g         2179 t
ORIGIN      
        1 gccagctggt agaggcggta gatggctttg gcgaacacga catagacgcc tggttgtggg
       61 gcgagtggca cggagatagg gcggtagggg acaagacgac cgaacgtcca cagaccctcg
      121 gcgttgtcgg aaagctgcag cggaggcgtc acctctcggt cgaggagttc gtctatgcgc
      181 ggggccgtac ggagaggacc gtgaaggtta cgctgccgag cccgagcatg tacgcgaacc
      241 tctggtcgcc agagctgtcc gcaggagtct acccgacgct cgatctcttt ctggaggacg
      301 tggcgcggat cctgcgcgag gaggtcgagg agttggtgcg gctcggtgcg gagtacatcc
      361 agctcgacgc gccgcattac ccgctactcc tggatcccaa gacccgcgcg ttctacgagg
      421 ggctggggtg ggacctggat cgctggctga gtcgggggat cgagctcgac aactacgtga
      481 tcggggacca cgatgacgtc acattctcct tccacctctg ccggggcaac caggggagcc
      541 gctggctggt ctcaggctcc tacgagccgc tcgcgcgccg catcttcggc ggcataaacg
      601 cgcagaggtt gatgttagag tacgacgacg aacgctcggg ggatttcgcc cccctctcgc
      661 acgtccccga ggacaaggtc gccgtccttg ggctcgtcac caccaagaca ccccggcgcg
      721 agacgaaaga agagctggag gcccgactcc acgaggcagc cagattcatc cctctggagc
      781 ggctggccct gagcccgcag tgtgggtttg ccacatccat tataggcaac gccataagcg
      841 tcgaagacga ggaatacaag ctacgtacca tagtcgagac agcggagcag gtctgggggt
      901 agacggggga gagggtcagc cggtgaacag gccctttaca ttcgatctca ggcgtccatc
      961 ttcgtcgagg tagtcgcgcc aggaacgttt gggatcgtag ccgagcatgc gtcttgcttt
     1021 ctcgatagag atgcctgagg agtcctcgcg ggagaggggt gtctttactt cgacctgctc
     1081 cccgtagtgc tcgcgcacgg cggcctcgaa cggcctgccg cccacgttat ccggcgaggc
     1141 gatgtagaag acctcgtggt ctggaaggtc gctctcggcg gcgagcacca tggcgtcagc
     1201 caggtcgtag acgtcgatgt agctccagaa gcccgcgctc agctccgagc ggtcgcgtaa
     1261 catgggccca aggtttcgct cgtagttgcc ttcgtgctgc acccaggaag ggcggatcga
     1321 gatgcaacgg atgtccgagc gccggatggc cccgtccatc agaagctcgc cgaaatactt
     1381 ggccgtcgcg taaggatcct gcggacggat cggatgttcc tcgtccacgg gcacgtaatc
     1441 gggaaggaaa ggccgctcgg ggaagaagaa ccctggcacc gtctcactgg aggtgtttac
     1501 gaaacgggtc accccgaaac gaatcgccgc ctccagggta ttgaacgtcg ccatgaggtt
     1561 gttctggaac acgacgtgtg gcgggttgct ggttggctcg gggatcgcgg ccgtgtgcac
     1621 aacggcctcg gcgccccgca cgacggcgaa ggcgtcgccc gcgtcagaga ggtcggcctg
     1681 gacgtagtcc gcctcgcctt cctcgggccg ctcgaagacc ggaggcgtca ggtctgtggc
     1741 ccgtacgtcg tgccccgcca cctgtagcgc cgccaccgtg gcccttccaa ccttaccccg
     1801 cgcacccgtt accagtactc gcatagatcc tcctgatctc gctcccggcc gatggtacaa
     1861 catcccgctt tcggcgtttc acccttcggg gagcagggta aaggcacttc atgagagtcg
     1921 tggtggttgg tgcgaccggc aacattggga cgagcgtgct gcgctccctc gagaacgagg
     1981 aaagcgtcga atccgtcctc gggttggcgc gccggatgcc ggatcttacg gtgccgaagg
     2041 tagagtgggc ctcggctgac gtggtaagtg aagaccttgt ccctcacttt agaggcgcgg
     2101 acgccgtggt gctccttgcc tggctcatcc agccgtcccg gaatctcaca aagcagtgga
     2161 tggtgaacgt cgaggggagc acgcgggtcg ccagggccgc aaaggaggct ggtgttccct
     2221 cgctccttta tgcctcgtcg gtcggcgcct actcggccgg gcccaaggac cgccgcgtgg
     2281 atgagagctg gccgaccggc ggcgttccga gctcttacta cggccgccac aaggccgagg
     2341 tcgagcgtag gctggaccgc ttcgaggcgg aggcgcccaa agtgcgggtc gtgaggatgc
     2401 gccccgggct cgtcttcaag aaagaggcgg cggaggggat aaggcgcctc ttcgccggac
     2461 ccttcttccc cagcgtgctc gccaacccgg ctctgataaa tgtcatcccc gatatagaga
     2521 acctgcgctc ccaggtgatc cactcctacg acgtgggcga ggctttcagg ctcggcatcg
     2581 taaacgggga cgttcgcggt ccgttcaacc tcgcggccga gcccgtcctc gacgcggggg
     2641 agatcggacg gatcctgaac gcccgtcccg tgccgacgtc cgccggggtc gttcgcgccg
     2701 gcgttggtct atcctgggcg ctgcgcttgc aacccgtgcc gccggggtgg ttggacctct
     2761 cgcgaaacgt ccccgtaatg gatactacga aggcccgtac ggagctcggc tggatcccgc
     2821 ggtacagtgc tgacgaggcc ctgctcgacc tcctcgaagg attgcggacc ggctcgggcc
     2881 tcgacacgcc accgctctcg ccgaagacgg gcggacgatt ccgaatacgc gagatgctga
     2941 ccggaatagg agggagggag ccttgaaggc cgtagtctgg cacggcaaag aagacgtcag
     3001 ggtggataac gtccccgacc cgataatcca ggagcccacc gacgccataa tccggatcac
     3061 ctcgaccgcg atctgcggct cggatctgca cctctacgcc aagctctcgc cgctgatgcg
     3121 cgagggcgac atcatcgggc acgagccgat gggtatagtc gaggaggtcg gctccgatgt
     3181 cgagcacatc tcgcccggcg atcgcgtcgt ggtccccttc aacatctctt gcgggcactg
     3241 ctacttctgc gaccagcaac tattttccca gtgcgagaac acgcgcgatg aaggcaagct
     3301 cgccaggtcc gtgaacctgc tgggacgcgg aaggggggcg agcctcttcg gctacaccca
     3361 cgtctacggc gcggtccccg gcggacaggc cgaatacctg cgcgtgcccc aggcccactt
     3421 cggtcccatt aaggtgccag aaggaccccc ggatgagcga ttcctctacc tctccgacgt
     3481 gctgcctacc tcctggcagg ccgtagccta cgcggatatt cccgagggcg gaaccgtagg
     3541 gatatggggc ctcggtccga tcggccagat gtgcgcccgc attgcgatgc accagggcgc
     3601 cggacgtatc ataggcatag acaacgtgcc cgagcgtctc gccatggcct ccaggcacgg
     3661 cgtccagacc gtggattact ccgcggtgga cagcgtgagg gatgtgatcc tgaacctcac
     3721 cgacggccga ggcgtggact cggccataga cgccgtaggt atggaagccg ggggctcgct
     3781 tgttggctcc atcttacaga gcaccaagct gcagctcgac cgggcgaccg ccctacgtga
     3841 cagtatgtcc gcagtgagac gcggcgggac gctctcgctc accggcgtct acgcgggccc
     3901 gatccaggct ttcccactgg gggacctctt cgacatgcag attcaactcc ggatgggaca
     3961 ggccaacgtg tggcagtggg tcgaggacat cttgccgctc ctgacaggcg acgatgatcc
     4021 actcggcgtc gaggatttca agacccacga gatgccactc gaagacgccc ccctgggata
     4081 cgagatgttc cagaagaagc aggacggcgc catcaagatc gtgctgaagc cgtaatcgac
     4141 gggccaacta ctagttcggc atcgtcagct tcacgccgtc gccatcgaac agggcgcaga
     4201 cagagtcctt cgtcacgggc ctcaacccga gcctgaacct cggcggcggg ttctacgcgg
     4261 gcaagcgggt ctctgtggac gccggcgaca acttcgtcag ctgaacgcac ccggcggggt
     4321 tctagaccac cggacgaaag gaggacttct cacggcagag ccgttcatag gtgagctccg
     4381 catgataacg ctcaaccaca gccaggcgct gttctccctg atggacgccg cctatggggg
     4441 cgatggcagg accacgttcg cgctccccat ccttcctgga gcgtgacgtt cctctcggtg
     4501 gaaagcttcg tttagctcga gacttcgtag accaatacga tttgtttggc tgcctcgttg
     4561 aggtcgtcgt cgaacgagag gaaccggaga tcccgttgcc ggccgtggca cacaagtgcc
     4621 gaggccaact gtatcgagtc gtagcctcgt agcgcgtagc gttgggctaa gtcgccagcg
     4681 agccggatca ggttctccgt tatcggtggc cgctcgtagg acggccaccg atggtcgagg
     4741 gcggcaacga cgccggtatg ttcctcctcg gtcagtgcct cttcccttag caaccgggcg
     4801 aacgtggccc tggcttcgac gtaggagacc atcgaggtcg cgatcttcga gctctccttg
     4861 acggcccgga ctaccagctc gcggcctcct tcgctcacga agaccttgac tagggccgac
     4921 gtgtccaggt agaggatcag cgacgatcct ctatgaccat ctccgcagcc gtcttgtttc
     4981 cgcgcaaggt cggcgcgtcc tcgtacaccc tcggcctctc accggcgacc ggctctccag
     5041 aagccgggta accgcgccgg ctcaggtact ctctgagagc ggcctgcatc accgcggcga
     5101 gcgagggtgg aaactccaga tctctcgcgt aggaatccaa ggccttctcc agcccatccg
     5161 ggatcgttac cgttgctcgt ctcatgccag gaatcctaac acagcgtgca ccggtcggtg
     5221 taatgtgcac caatggagga gcccgatcca ggaagcaagc ggttaagctt tgggttagaa
     5281 cgacagagaa caggtgcgcg ccggctctag cgcaccgact ccaccacggt ctgccagaag
     5341 gcgtcacgct cgcctggtac gaaggggata tatagcgtga cgcggtcaat gagcccggtg
     5401 tagcgctcct cgagtgcgct cccgatgtca tcgggtgcag cttcgacggc gaacgcggcg
     5461 agcatctcgt cggttacgag ggcgggcatc tcgcgccact tcttttcacg ggccatcgta
     5521 ccaagtttct cgccgacctc ttcccagccg tgggcttcga ggaccgtgcg gtaggtgggg
     5581 gtcgaagcgt agaaggagat ctgcgcccgc accgactccc gctgctctgc ggcctccact
     5641 tcgctctctg cggagatgac gaaagcgctc gtcgctagcg tgacttgccc cggatctcgg
     5701 ccagctcctt cggctcctgc ggttatggcc ggtattacgg ttctccgcac gtactcggga
     5761 gagtggaagg ggtggacgtg gaagccgtcg cagatctcgc ccgcaagcct ggccagacgt
     5821 gtattgacgc ccgcgatgta gaccgggatc tgagggtgat caattggtcc cgggttgaag
     5881 aacggcgtca tcagggtgtg cctgtagaat tcgccctcga acttgagggc accctcgttc
     5941 tggaaagaat cccagatctc acgcagggcc ataatgtact cgcgcagccg tgccgccggc
     6001 tttccccagg gcatcgagag gcggcgcgta acatgggctt ttacctgcgt gccgaggccg
     6061 agaacgaagc gtccatcgga gaggttttgg agatcccagg ccgtctgcgc cgtctccatc
     6121 ggcgagcggg agaaggcgat ggctacggag gtacccaact ctatctcatg ggtttcgttc
     6181 gcggcaatgg ccaggggcag gaacgcgtcg tgtttggtct cgctggtcca caggccggag
     6241 aagccaaagt cttcggccgc gcgggcggtc ctgtctatgc ctttgaggta atcgccctct
     6301 gtgccgagtc cggcgtcgag tttcacggtc tcttgctacg ggatcaggag gagcttgccc
     6361 gtggtcttgc ggccttcgag gtcgcggtgg gcctgctcta cgtcttcgag ggagtacgtg
     6421 ccgccgatgc ggacgttgag gttgttctgt ccgatccagg agaagaggct ctcggcgcgc
     6481 cacaggagct cctcgcgggt cgcggtgtac tgggcgaggg cgggccgggt gaggtagagg
     6541 ccccctttct ggttgagggt ctgggggtcg aacgggggca cgggaccgct ggatgccccg
     6601 aatagcacca tgtagccgcg tgggcgcaag acgtcgaggc tgccatcgaa cgttgctttg
     6661 cccaccgagt cgtagacgac gtggactcct tccccgcccg tgatacggtt cgtctcctcg
     6721 acgaagtcct gctcggtgta gaggatgacc tcgtcggcgc ccgcctccct ggcgagcttc
     6781 gccttctcct cggtgcccgt ggtcccgatc acggtcgccc cccgcagctt cgcgatctgg
     6841 acgagcagga gcccgacgcc tcctgcggct gcgtgcacga gcgccgtctg accctcctgt
     6901 agcgggaagg tgctgtgcgt caggtagtgt gccgtcatgc cctggagcat gacggccgcg
     6961 gctacccgcg cctcgaccag cgtggggttg aaaggcacca ggttctcggc gggggcgacc
     7021 acgtactctg cgtagcatcc cggggtgctg gcgaacgcaa cgaagtctcc cggcgacacg
     7081 tcacctgcgt cctcacccac ggcgtccacc tcgcctgcgc cctcgagccc gagcgtacgt
     7141 ggtaggtcgc cggggtagag gcccgtgcgc tggtagacgt ctataaagtt gacgccggac
     7201 gccgcgagct ttacgcgcgc ctggccggcg cccggttctg gggtctccac atcctcgtag
     7261 ccgaggacct cgggtccgcc gaactcggtt atccggatcg ccttcatgct tctctacctc
     7321 ccgcggtgaa ctcgacttgc acgcccgtca ttgtggcagc atttcagcac ttcagcacgc
     7381 tcaggccttg cagccttcgc tcgtcagcat ttcagcttgt cagctctttg ttcttgttgg
     7441 aagcttatcg ctcgtaggcg gatgtatggg tcgtggcggg tcgattgctt cgcggaatcc
     7501 tctagtcctc cccggattct ccacttcgac ctcgtcctcg accgcgacct tgtcctccgc
     7561 tgttatccgc tggttctttt gtcggcgcag ccgagttctg cttttgcgca gaaggactct
     7621 ttttcacctt gggtttggct ccgttgggtc cgttggcctc agggccattc gtgccggccg
     7681 cctggcgatt ctcgcgcggg gtaggccggg ccgaggcgga ggaggttgcc gaggcggaag
     7741 cgccgggttc gggactcgac tgggcggacg cggacgagct cgcggatgag gaaggttgtg
     7801 ctcccgagct gatggtcagg ttcacgtctg tacctggatc gactggtgtg cccgcggcga
     7861 tgcctggctc gatgaccctg cctgcggcga cgctatcgct cgggaacgag ctcacctgcc
     7921 cgaccgcgag ccccgcgtcg gtgagcctct tctgggccag gctcctgggg agtccgtcca
     7981 cttcgggcac ctccacctgt ccagcgggct ggccaggagg tcgctgttct ccctggaaga
     8041 cgtcgaacca gccggccgtg gcatcgggac ccagaacggc ctgcacgccg ccgagcgtgc
     8101 cgagggtaaa caggatcgct gccagcgcga tcagcaacca gggcagacga cgcctgcgtc
     8161 gatacgccac cgggcctggc cggggtctgt gcatcttgtt cgtgacgttc tgcgatgcgg
     8221 gggcattgcg ctgcgtgatc tgttcccgct tccttaagac accggccgaa accggcttga
     8281 gtccgcggtt cacccgccag aggtcttccg caagcacgtc ggcgtctgca tagcggtctt
     8341 ccgggtcttt cgccaggagc ttcgtcacga tggcgttcat gcccctgggt atgcggggat
     8401 tcagttctat ggggggccgt aacggctcgt tgacgtgctt catggagacg gcgatcgggt
     8461 tgtcggctgt gtaaggcagt tcgcccgtga gcgcttcgta gagcaccact ccgagggagt
     8521 agaggtcgct cttgggggtc gcgggcttgc cgagggcctg ttcgggggac atgtagcctg
     8581 cggtgcccaa tacggacccc gtcctggaga tggtcgcgga ggagccggcc ctggcgatgc
     8641 cgaagtccgc caccttcgcc ccgcccctgg ccgtgagtag tacgttctgg ggctttacgt
     8701 cgcggtggac catgcccctg tcgtgggccg ccccgagcgc cccggcgacc tgcgccacga
     8761 cgccggcggc gtccgccgcc tcgagcgggc cctccctgga gatcctctct ttgagcgtgc
     8821 cgccgggcac gtattccatg gctatgtagg aggccccttc ttccgtctcg cccctgtcgt
     8881 agacctggac gatgttaggg tgggagagcg acgccgcgct catggcctcg cgcttgaagc
     8941 gctcggcgaa ctcatcgtcg ccggcatact gtctcctgag aaccttcagc gccacgtcgc
     9001 gcccgagcac cctgtcacgg gccaggtaga cctcgcccat gcctccgctg ccgagtggtc
     9061 gaactacgtc gtagcggtca tctaagactg ttcttctcgt ctctgacatc ctgcccttgg
     9121 tcctgctcta aaacgtcaac cgcgttattc tacacatacg catgggacct tacgaaggat
     9181 gtttcacaag agagcgaggg ttaggcggcg ccagggtcgg gtactgtaca agtgccaggt
     9241 cgaattccga tcggaggact cggcggggcg aagaggcaga cgacgagacg gtggggacaa
     9301 taggggtggg atcttggcta caagcggagt agaagtagac gcgatgcggc ttctcggcgc
     9361 cttctacgac ctctcggagg gaaggttaac cgagccggtg ccgatcgggg atccagaacc
     9421 cggcgcggca ccgagggccg atctcgatcc cgacactcca gagtgcgaca tcgcgatcag
     9481 gtacctggtc gatcagagat tcatcgcacc ggcccacgcc gcgtccgagt acacgatcac
     9541 cgtcgccggc tttgacaggg taagagagat gcgcgggata ggcggtcctg aagcaccggc
     9601 agggaggaac aggttgagcg ataaaacaca gaaacagcta atgacgttgc tttccatagg
     9661 gatctccatc ggcctcagcc agccgctggc caggtttatc gacaagaata tccccgaacg
     9721 caggggcata aaggacgacc tgaccgaggc tgtcctgcaa gggcttgtaa gggtcgtggc
     9781 gctcgtggtc gcctccgtga tcgtgcgccg cctggccgcg agccgtaggt aataccgagt
     9841 ccgggagttc gcttcgtccg ttcttagggc gtaagcatgg ggagtagggg cgggtttgaa
     9901 acccgcccct acgagggaga ggagatcctc cgagtggctg acgtcttcgt cgccggttct
     9961 ataaaccagg acttcgtcct gagcgtacaa cgccgtcccg cgcccggcga gacggtcact
    10021 gacgcccgtc tcgcgaccca caacggtggc aagggggcca atcaggccgc cgccgcggcc
    10081 cttctagggg cgagtgtggc attcctcgga cgcgtcggcg acgacgggtt cggcgaacct
    10141 ctggtacggg cgctcgcaga taagggtgtc gatacgggac ttatgcagga ggttcccggt
    10201 tcatccacag gcgcggcctt cataaccgtc acccctgatg gggagaacgc gatcacggtc
    10261 gcaccgggcg cgaaccgccg cctcaccgcc gaagacgtag acgaagcctc cgagtccata
    10321 ggagatgcca aggtactcgt cgcccagatg gagatccctc ccgaggtcgt cctgcgggcc
    10381 gccgaggtcg cggcagcgag cgggacccgc gtgctggtca acctcgcgcc cccgttcgag
    10441 gttccgcgga cgctgctcga gaagctcgac cccctggtta tcaacgagca cgaggccgcc
    10501 ttccttctcg gagagagggt cgaaggcgta gatgaggcgc tcgatgccgc cccggggcta
    10561 ctggcgctgg ggtcgggatc cgtagtggtc acgctcggag aagacggcgc cgtcttctcg
    10621 gatggcgaat ccgcgcagca cctcactgcc ccgaaagtcg aagtcgtgga cactacgggt
    10681 gccggagacg cgtttgtcgg ggccctggcc gcaagactcg ccgccgacgc acccctgaaa
    10741 gacgcggttg cctacgccgt acgggcgggt gcggcggcgg taacggaggc gggcgcccag
    10801 ggagcgctcc cgagccccga tgtggtcgag gccttgtaga gcgtcaaccc cgtcgcgtag
    10861 accccgcgaa gaagacgggg ccgtacgtct ggggcgggtc tggctcgaag cccgcgtggt
    10921 cgagcaggct gtgtagctcg tcggggtcga agaacctgat gccgcctgtg gagagcaagc
    10981 gctggagccg gcgtccgcgc ggcgtggcga cgcgcaggat gcccatgatg gcaaggcgcc
    11041 cgccgggttc gaggacccgg gaggtctccc gcaggactcg tgcgggatct ccaaactcgt
    11101 tgagggtgcc gccgcagacg gcgcccgtga aagaggtgtc tgcgaaggga agttgcttcg
    11161 cggtcgcccg gatgaaggac ggcttcggct cgaccgcatg tgcccgtcgg accgcttctc
    11221 tcagcataga gggggatatg tctattccta cggcgatgcc gctgtcgagg ttggcgctga
    11281 ggctacggga atacagtcct gcggagcagc ccaggtcgag gtagcggccg cttctgacgg
    11341 gacggacgag acgatggatg atctccagct cccgctcgtt cgggaaggat tcgccggtga
    11401 gcagactgag ggagcgcacg cgccagagcg gttcgtagcc gcgtccggcg ccgggcagga
    11461 agttcgtgag attggcgaag ctgtccgcgc cggtgcggga cttcaggaga tcaaggtatc
    11521 cgtctttgac ctcgtaggtt tcacccgtcg ccggagagcg ggctttgccg cccaggatca
    11581 taccgtcact ctcgatacca ccggcgtaga ggagaccctc ggtatccgaa ggacagggac
    11641 ggacgatgga gagcaactcc ttgcttatca tctagcgtcg tcttctgtcg cgcaggcggt
    11701 tcaggcgcgc gtcgaagccc ctgacggagt cgacgaaggc gcccgtttcc tcgggacgga
    11761 cggcggattt cagggtcggg taggcgccct cgtggaagat cagaggtccc ttgcg
//
LOCUS       DATMVF010000183         5577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_397690, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000183 DATMVF010000000
VERSION     DATMVF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397690"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VN178_09070"
     CDS             complement(<1..348)
                     /locus_tag="VN178_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWS81163.1"
                     /translation="MFTRAKTEPVPEVGAEAPEFNLPSAQGGQLRLSMRTPRGPVVVA
                     FYRPADEEDVEYFKALAAKEEEINLAAGSVVAIGVAEPTQALNFARASGLKSYVLYDY
                     ARVTSRQWGLLEGD"
     gene            645..2192
                     /locus_tag="VN178_09075"
     CDS             645..2192
                     /locus_tag="VN178_09075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWS81164.1"
                     /translation="MRMIMTHGYMLSGTGSNIYVQSLCRALVGAGHDVHLICQEPDPL
                     AYDFVGMSAKVGAQGVEDLGEQETPYSGHCTLYRPEIGDLLPVYVYDDYPGWRVKTFL
                     DLTQEELDYYLGHNVEALKTVLGVSGAEAVITNHSVPGPLIARRALEGSELPYVSIVH
                     GSCLQYVSRKSEKYMALTREGLEDAREILALSSHSAGTISEDFPDLADRTHSLPGGVD
                     TDLFRPDAFDRGVFDHLEGGPGRGPAQRASLREALESSGHAGELAGALRAVAASYDAR
                     SHDTDAGERLRDFLDREGPLLVYVGKLIHSKGVHSLISAFSRVRKETGARLLVIGFGT
                     FREGLEALTCSLSDGDEATVERLAEMGKLLEGGPAGPLEHFDLADEMLVDAEGMQDDV
                     LFVGPLYHGDLTKVLPAADAAAVPSIFPETFGLVAAEFAASGVVPFVSDHSGLREAGE
                     IVGRGLSFDLRVGMDGFEENFAEALNDYLKLPEKERRRCEEVVRRNCVEHLSWGTLAE
                     KLGALVG"
     gene            2298..3418
                     /locus_tag="VN178_09080"
                     /pseudo
     CDS             2298..3418
                     /locus_tag="VN178_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            complement(3479..4801)
                     /locus_tag="VN178_09085"
     CDS             complement(3479..4801)
                     /locus_tag="VN178_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HWS81165.1"
                     /translation="MPRRLSTPRLIVVGFLALMALGTVLLKLPLSTERGISWIDAMFV
                     SVSAGSVTGLSTVNFPATFTTFGAVVVMVLVQLGGLGIMTVTTMAALFVGQRVGFRNL
                     IAVRESIESVDSPRNVLRLLLQIARITFAVELLAAVALSVAFIRRGMAPLEAVFQGFF
                     HAIMGFCNAGLVNLESGSFIPYAGDWLVVGTLSLAIILGGLGFPVLVDLYRYRKGRRL
                     TVHSRLVLITSAVLLVVGIVSVAAMEWSNSGTLGGQSLSTRLAMSVFQGVTARTAGFF
                     TVSYPEMRDPTLVVQTLLMFVGTAPTSTGGGIKVTTLALVFLIIVSQVRGQDRITLFW
                     RTLPRSLVAKALSVLALASLLVFLSTLALMVSDGLEFLSALFEVTSAFGTVGLSLDVT
                     PTLSTFGKVLISAVMFLGRVGPITFIVALAVRQHTSHYRYPEEEIAIG"
     gene            4956..>5577
                     /locus_tag="VN178_09090"
     CDS             4956..>5577
                     /locus_tag="VN178_09090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWS81166.1"
                     /translation="MTQLTDSYNRKMDYLRISVTDRCNFRCQYCMPEDIQFQDKSHIL
                     TLEEMLTFAEACLELGVTKVRVTGGEPLVRRGVVMFVGWLKDLGFEDVTMTTNGFLLE
                     ENLEGLVEAGLDRVNISLDTLQPEKFAFITRRNHFQKVWRAVMAALETPLSPVKINAV
                     AMRGVNDDEIADMARLTLEYPMHVRFIELMPLNGDTDGSRFRSLFIS"
BASE COUNT         1025 a         1815 c         1759 g          978 t
ORIGIN      
        1 atccccctcc aggaggcccc actggcggct cgtgaccctg gcgtagtcgt agagaacgta
       61 ggacttcagt cccgaggccc tggcgaagtt gagggcctgg gtaggctcgg cgaccccgat
      121 ggcgaccacc gagcccgcgg cgaggttgat ctcctcctcc ttcgcggcca gcgccttgaa
      181 gtactcgacg tcctcttcgt ctgccggcct gtagaaggcg accacgaccg gtccccgcgg
      241 ggtcctcatg ctcagcctga gctgtcctcc ctgggcgctg ggcaggttga actcgggcgc
      301 ctcggcccca acttcgggca ccggctcggt cttcgcgcgt gtaaacatgc cgtcctcctt
      361 gcgagtatca agaatcgtac acgcgaatac tacagcctat ctcagcacgc tgcgcccttg
      421 cgggctccgc tttcagcatg tcagcacgcc gcctacggcg gcttgtcagc agttcagcaa
      481 aaagctgact agctgaagcg ccgagagccg ccgaccgcag ggaggcggcg tgctcacaag
      541 cggagacggc agccggagcg tgctgagtct ggggcgcgac tctgtgatac cttccgctat
      601 acgagcatgg agatgcgaat tccacacggg ccagaaggag cgccatgagg atgatcatga
      661 cccacgggta catgctctcc ggcacgggga gtaacatcta cgtccagagc ctgtgtcgcg
      721 ctctggtcgg cgcggggcac gacgtgcatt tgatctgcca ggagccggac cccctggcct
      781 acgatttcgt cggtatgagc gcgaaggtag gcgcacaggg ggtcgaggat ctgggcgagc
      841 aggagacacc ctactcgggc cactgcacgc tctaccggcc cgagatcgga gacctactac
      901 ctgtctacgt ctacgacgac tacccgggat ggcgcgtgaa gaccttcctg gacctgacgc
      961 aggaggaact ggactactac ctcgggcaca acgtcgaagc tctgaagacc gtgctcgggg
     1021 tgtctggcgc ggaggcagtg atcaccaacc actccgtacc tgggccgttg atcgcacgcc
     1081 gtgccctgga gggcagcgaa ctcccctacg tgagcatcgt ccacgggagt tgcctccagt
     1141 acgtctcgcg caagagcgag aagtacatgg ctctcacccg cgaaggcctg gaggacgcgc
     1201 gggagatcct ggctctctct tcgcacagcg ccggcaccat atccgaggac ttccccgacc
     1261 tggcagacag aacccactcc ctccctggcg gggtcgacac cgacctcttc cgccccgatg
     1321 ccttcgaccg cggggtcttc gaccatctcg aaggcgggcc gggccgtggt ccggcgcaac
     1381 gggcgtcttt gcgcgaagcc ctggagagtt cgggccacgc gggagagctg gccggtgcgc
     1441 tgcgcgctgt ggcggcctcc tacgatgctc gctcgcacga cacggacgcg ggcgagaggc
     1501 tgcgggattt cctcgaccgc gaggggccac tcctggtcta cgtcggaaag ctgatccact
     1561 ccaagggcgt gcacagcctg atctctgcct tctccagggt gcgcaaggag acgggcgccc
     1621 gcctgctggt catcggcttc ggcaccttcc gcgaggggct cgaggcgctg acctgctctc
     1681 tcagcgacgg cgacgaggca actgtagaac ggctggccga gatgggaaag ctactagaag
     1741 gcgggccggc tggtccgttg gaacatttcg atctcgccga cgagatgctc gtcgacgccg
     1801 aaggcatgca ggatgacgta ctattcgtcg ggcctctcta ccacggcgac ctgacgaagg
     1861 tacttcctgc cgcggatgcc gcggcggtac cctccatctt ccccgagacc ttcgggctcg
     1921 tggccgcgga gttcgcggcg agcggcgtag ttcctttcgt gtcggaccac tctggcctca
     1981 gggaggcggg tgagatcgtg ggccgcggtc tgtccttcga cctccgcgtc ggcatggatg
     2041 gcttcgagga gaacttcgca gaggcgctaa acgactacct gaagctgccc gagaaggaga
     2101 ggcggcggtg cgaggaggtc gtgcgccgga attgcgtgga gcacttgagc tgggggacgc
     2161 tggcggagaa gctcggagcc ctggtcggct agaaggtgga ggagatagag cacatcctcc
     2221 gcgacttcgg cctcatactg ggcgccggac tggtctcgca gctcatagcg accgtcacaa
     2281 ggctccccca gacggtcgtg cttgtcgcgg cgggcgccct tataggacct tccgtgctcg
     2341 ggctcgtctc gaacccgctg aacggggtgg gtgcccaact cctattcaac ataggggtgc
     2401 cctcatcctg tttcacggcg gcacaggcat ctcgctgcgg gtgatctcgg gtaccgccgt
     2461 gggcctcggc ctgctcgtct tgcccggcgt acaactgacg gccatcatcg tcgcgctggt
     2521 ggtctccccc gtgttcggcg tcgccttccc tgttgccctg ctcgtggggg cggtcctgtc
     2581 cgcgacggac ccggccatcc tcatccccct gttcgaccgc ctgaaactcc gccccaaagt
     2641 gtcccagaca gtaatcgccg agtcggcctt caacgacgtt acaggcacgg tgcttaccct
     2701 gacgctcgtc ggcgtcgtgg aggccggcgg attcacgttc tctggtcctg ctctggagtt
     2761 caccaaagaa ctcgtcctcg gagccctgat cggcgtcgtc gccggggctg tcctctgcta
     2821 cgctgtcgct tcgacggcgc gggccgggat atgggacgag tcaccggggg tggccatcct
     2881 cgccgtcgtc gcgctcgcgt acttctcgac cgagtctctc ggtggatctg cctacctcgc
     2941 gacgttcgtc atggacctga tggtcggtaa catggagtat ctcaggctcg gccaccacga
     3001 cgagcacgcc acgcttctcg aaggcttcct cggacaggtc gctgagatag ccaccctgct
     3061 cgccttcgtc acgctcgggc tcaacctacc cttcgaggcg ctcggaaatt acctggtcgg
     3121 cggactgctg gtcatgttcg tattcatctt cgtggcccgc cccataacag ttctcgcgtg
     3181 cctcttgccc gaccggcgtg gcggctggac gggaagggag atcgccttta tcagctggtg
     3241 cagggagacg ggtgtgatcc ccgccgccgt cgcgagcctg ctcctcgcgc aaggggtcga
     3301 aggcgcggag atcgccgtct ccatggtcgc cctcgccgtc tgcgttactc tcttcctgca
     3361 agcgacgacg gccggctacg cagcccgccg tctcgggttg atcgaaagct caggctgacg
     3421 ccttcgcttt cggcacgccg cctgcggcgg cctgtcagca tttcagctgg tcagcttgtc
     3481 aacctatcgc tatttcctcc tcggggtatc gataatgtga agtgtgttgc ctgactgcca
     3541 gggccacgat gaaggtgatc gggcctactc tacccaggaa catgacggcc gagatcagga
     3601 ctttgccgaa ggtactcagg gtcggcgtga cgtcgagcga gagccctacg gtaccgaacg
     3661 ctgaagtcac ttcaaagagg gcggacaaga attcgagccc atccgagacc atgagcgcga
     3721 gggtactcag gaagacgagc aaggaggcca gggccaacac ggagagcgct tttgcgacca
     3781 gggaccgggg cagcgtgcgc cagaagagcg tgatcctgtc ctgccccctg acctgcgaga
     3841 caatgatgag gaacacgagc gccagggtgg ttacctttat gccgccaccc gtcgaggtcg
     3901 gggccgtgcc gacgaacatg agcaacgtct gcacgaccag ggtcgggtcg cgcatctcag
     3961 ggtagctcac ggtaaagaaa cccgccgtcc gcgccgttac gccctggaac acggacatcg
     4021 ccaggcgggt gctcaaggac tgccccccga gcgtgccgga gttgctccac tccatcgcgg
     4081 ccacgctgac gatcccgacg accaggagca cggccgaggt gatcaagacc agccgcgaat
     4141 gtaccgtcaa ccggcggccc ttcctgtaac ggtagaggtc gacgagcacc gggaaaccca
     4201 gtccacccag gataatggcc agggacagcg tcccgacgac gagccagtcc cccgcgtagg
     4261 gtatgaagct gccgctctcc aggttcacca ggccggcgtt gcagaagccc atgatcgcat
     4321 ggaagaagcc ctgaaagacg gcctccaaag gagccatgcc ccgccggatg aaggccacgc
     4381 tcaacgcgac ggccgccaag agctcgacgg cgaaggtgat tctggctatc tgcaacagga
     4441 gccgcagaac gttgcggggc gagtccacgc tctctatcga ctcgcgcacc gcgatgaggt
     4501 ttcggaagcc cactctctgc ccgacgaaca gcgccgccat ggtcgtcacg gtcatgatcc
     4561 caagcccccc aagctggacc agcaccatga ccacgacggc cccaaacgtc gtgaaggtgg
     4621 caggaaaatt caccgtcgaa agaccggtta cgctccccgc gctaaccgag acgaacatgg
     4681 cgtcaatcca tgatatgccg cgctcggtgg agagaggcag cttgaggagc acggtcccaa
     4741 gcgccatcag cgcgagaaaa ccgacgacga tcagccgggg cgtcgaaagc ctcctgggca
     4801 acaccttctc ccggatagtg gccacgcaaa gactgcgacc ggaggtcgca ggcccagggc
     4861 agtatacccg cgggcgattc ggccgctact ctcgtctccg aaagaagata caatccgacg
     4921 agagccagtg atgggcgcaa gcccgaaagg atgctttgac gcagctcacc gacagctaca
     4981 accgcaagat ggattacctc aggatctcgg tcacggatcg ctgcaacttc cgctgccagt
     5041 actgtatgcc ggaggatatc cagttccagg acaagagtca catcctgacc cttgaggaga
     5101 tgctcacctt cgccgaagcg tgcctcgagt taggcgtcac aaaggtccgg gtaacgggcg
     5161 gcgagccgct cgtcaggcgc ggggtcgtca tgttcgtcgg gtggctcaag gatctcggct
     5221 tcgaggacgt aaccatgacg accaacggct tcctgctgga ggagaacctg gagggcctcg
     5281 tcgaagccgg cctcgaccgc gtcaacatct ccctcgacac ccttcagccc gagaagttcg
     5341 ccttcattac gcggcgcaac cacttccaga aggtctggag agccgtgatg gcggcgctcg
     5401 agacgccact ctcgccggtc aagatcaacg ccgtcgcgat gcgcggggtg aacgacgatg
     5461 agatcgcaga catggcgagg ctgacgctcg aatatcctat gcacgtcagg ttcatcgagc
     5521 ttatgcccct aaacggcgac accgacggct cgcgtttccg ctcgctcttc atctccg
//
LOCUS       DATMVF010000184         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_166919, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000184 DATMVF010000000
VERSION     DATMVF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166919"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VN178_09095"
     CDS             <1..289
                     /locus_tag="VN178_09095"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWS81167.1"
                     /translation="DADAGARYLGELGIGTNYGIRRPSANVLFDEKLGGTVHLAIGRS
                     YAQTGGKNDSSVHTDLVCDLRQGGELYADGELIQQNGRFLEFDLAGVNDAW"
     gene            279..515
                     /locus_tag="VN178_09100"
     CDS             279..515
                     /locus_tag="VN178_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81168.1"
                     /translation="MPGEAMLRVENVRDEAALESVRDALDRLGVDYEHVRSEPDDDRF
                     PQTAYFYVPDDSADVVESVLADLSREHGFDAEVL"
     gene            complement(654..1418)
                     /locus_tag="VN178_09105"
     CDS             complement(654..1418)
                     /locus_tag="VN178_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWS81169.1"
                     /translation="MNVFGIDIGGSGIKGAPVDTQTGELIEERVRIKTPQPATPEAIV
                     GTAVEVVREAGWEGPVGCGFPAVVKDGVVQTAANIDKAAIGFDLGEALRREIDAPVSI
                     VNDADAAGLAEVRWGAGREESGVVLMLTLGTGIGTSLFVGGRLVPNTEFGHIEVRGKD
                     AEHRAADSARKREDLSWKEYAGRLDEYLHRIEFLLWPDLIVIGGGISKKSEKFFPHLT
                     ARTKVVPAEMHNNAGIAGAALAGTRDRAAVQTAEKR"
     gene            1689..2462
                     /locus_tag="VN178_09110"
     CDS             1689..2462
                     /locus_tag="VN178_09110"
                     /inference="COORDINATES: protein motif:HMM:NF028265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81170.1"
                     /translation="MSWAIFLGAFVGSLIELVEVLAVVLVVGRVAGWRNALVGTGSAI
                     GLVAVAALAVGSGLALIPARPLELLAGVILLGFGQAWARSVTRYYGGVLETPDDDDER
                     IREQLARDGGRGGWDAVALATAFKSSLVESFEIAIVVIGLGTAGGKWLEAIGGAVVAS
                     VVLICSAFWLRASLERVPVKPAKFIAATLLMGFGTYWVGEGIGFGWPGGMASLIWLPL
                     LWGLLMAAGAVFLRSRRGEVAEPLEPGTHRGQEPPGGGP"
     gene            2480..3268
                     /locus_tag="VN178_09115"
     CDS             2480..3268
                     /locus_tag="VN178_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81171.1"
                     /translation="MVGLRGKVVAITGAGGIGSETARAFAEEGCRVAVCDRLVDRLES
                     ARERLEGGGGEVHMMLADVSDELQVRDFIEATVERWGRVDVLINNAGYGLHKPFFKTS
                     VEEVQAQLQTNYMGVVYATKAALEHMVPARRGHIINVVSVVAKLPVPNSGNYAATKAA
                     LDSLSSTLRAELSEYGIVVTTVYPTAVRNTHFFETSGSMVEETWARHFMKEPTEIAAA
                     IVGAAKSLRAEVHVGAQTRILPVARALLPPVVRLALRWIARVYR"
BASE COUNT          538 a         1030 c         1099 g          638 t
ORIGIN      
        1 cgacgccgac gccggggccc gctacctggg ggagcttggg atcgggacca actacggcat
       61 ccgccggccg agcgcgaacg tcctcttcga cgagaagctc ggcgggacgg tgcaccttgc
      121 tataggccgc tcctacgccc agaccggcgg caaaaacgac tcgagcgtgc acacggacct
      181 cgtctgcgac ctgcgccagg gtggggaact gtacgctgac ggggagttga ttcagcagaa
      241 cggccgcttt ctggaattcg acctcgcggg ggtgaacgat gcctggtgag gcgatgctgc
      301 gcgtagagaa cgtcagggac gaggcagcgt tggagagcgt cagggacgcg ctcgataggc
      361 tcggcgtcga ctacgagcac gtacgctccg agcccgacga cgaccgcttt ccccagaccg
      421 cctacttcta cgtacccgac gattccgccg atgtcgtaga gtcggtgctc gcggatctct
      481 cccgggagca cggcttcgac gccgaggtgc tctgaaagcg ttgcgcgtaa gcgacgacgc
      541 ttgggccgtc cgcgctggtt acggcacttc ggccgtcggg gctctccgtg caccagactc
      601 gagatacttg cacagccctc tcaaggcgcc cagcgagtag ggcgtaggct ggatcaccgc
      661 ttctctgcag tctgtacggc tgccctatcg cgggttccgg ccagagcggc tccggcgata
      721 ccggcgttgt tgtgcatctc ggccggcacc accttcgtgc gggccgtgag gtgggggaag
      781 aacttctcgg acttcttgct tatgccgccg ccgatgacga tgaggtccgg ccagaggagg
      841 aattcgatcc tgtggaggta ctcgtcgagg cgccctgcgt actctttcca gctcaggtct
      901 tcgcgcttgc gggcgctgtc cgcggcgcgg tgctcggcgt ccttgcccct gacctcgatg
      961 tgtccgaact cggtgttcgg caccagccgg ccgccgacga agagggaggt gccgattcca
     1021 gtacccagcg tgagcatcaa gacgacgccg ctctcctccc tgccagcgcc ccagcggacc
     1081 tcggcgagac cggccgcgtc cgcgtcgttt acgatactca cgggggcatc tatctctctc
     1141 cgaagcgcct cgcccaggtc gaagccgatc gcagccttgt ctatgttcgc cgccgtctgc
     1201 accacgccgt cctttacgac ggcagggaac ccgcacccga cggggccctc ccacccggcc
     1261 tcgcggacca cctccaccgc agtaccgacg atcgcctcgg gcgtggccgg ctggggagtc
     1321 ttgatcctta ccctctcctc gatcagctca cccgtctgcg tgtccacggg cgcgcccttg
     1381 atccccgacc ctccaatgtc gatcccgaac acgttcatgc gtcctccacg atcagatacc
     1441 gccgcctgcc acagccgtca ttctaaacga tcccaccccc acccgaacct ccctccaggc
     1501 gtctcgctga ctcttgtgac gtagatcagt tgttttcttc tcgccggacg tgtatcgtgt
     1561 cgatcatgcg gttattgcga atggctctca atagcggaca ctgaagtgac tcgtgtgaat
     1621 ctagcaatcg ggatgtgatc cgggtccgtg atctggaggg ctgagtctct ctccatggag
     1681 gattactggt gagctgggcg atcttcctcg gtgcctttgt gggatccctg atcgagctgg
     1741 tcgaggtcct tgcggtggtg ctcgtggtcg gtcgggtcgc cgggtggcgc aacgccctcg
     1801 ttggcaccgg gagtgccatt gggctggtgg cggtcgccgc cctggcggtg ggtagtggtc
     1861 tcgcgctgat tccggccagg cccctcgaac tccttgccgg cgtgatactg ctcggtttcg
     1921 ggcaggcgtg ggcgcgttcc gtgacgagat attacggcgg ggtgctagag acgcctgatg
     1981 acgatgacga acgtattcga gagcaactgg cgcgagacgg cggtcgcggt ggctgggacg
     2041 ccgtagccct ggcgacggct ttcaagagca gcctcgtgga gagcttcgag atcgccatcg
     2101 tggtgatcgg gctgggcacg gcgggtggga agtggttgga ggcgatcggc ggtgcggtgg
     2161 tcgccagcgt ggtcctcatc tgctctgcgt tctggctcag ggcgtccttg gagagggtgc
     2221 cagtcaaacc agcaaagttc atcgcggcga cgctgcttat gggcttcggc acctactggg
     2281 tgggtgaggg gataggcttc gggtggcctg gcggtatggc ttctttgatc tggctgcctc
     2341 tcctctgggg actcctgatg gccgctgggg ccgtgttcct gcgttcgaga cgtggcgaag
     2401 tcgcggagcc gctagaaccc gggacgcaca gagggcaaga gccccccggt ggcgggccat
     2461 aatacatgag aacggatcca tggtagggct gcggggcaaa gtagtcgcta tcacgggtgc
     2521 cggcggtatc ggttcggaga ccgctagagc cttcgcggaa gaaggttgcc gtgtggccgt
     2581 ctgtgaccga ctggtggacc gtctcgaaag tgcccgcgag cggctcgaag ggggcggcgg
     2641 tgaggtacac atgatgcttg ccgatgtctc ggacgagcta caggtcaggg acttcatcga
     2701 ggcgaccgtc gagcgttggg ggcgggtgga cgtgctaatc aacaacgcag gctacgggct
     2761 gcacaagccg ttcttcaaga cgagtgtgga agaggttcag gcccagctac agaccaacta
     2821 tatgggggtc gtgtacgcta ccaaagccgc gctggagcac atggtgcccg cccggcgcgg
     2881 acacattatc aacgtagtct cggtggtggc caagcttccc gtgccgaact ccggcaacta
     2941 tgccgctacc aaagcggcac tcgattcgct ctcctcgacc cttcgtgcag agctctccga
     3001 atacggcatc gtagtaacga cggtataccc gaccgccgtg cgtaataccc atttcttcga
     3061 gacatcaggc tccatggtcg aagagacctg ggccagacac ttcatgaagg agccgaccga
     3121 gatcgcggcc gccatcgtcg gcgccgcaaa gagtctccgg gcggaggtgc atgtcggagc
     3181 ccagacgcgg atcctccccg tggcccgggc gttactgccg ccggttgttc gtctggcgct
     3241 gcggtggata gcccgcgtgt atcgttagcc ccgaggggcc atcaatcggc ccacgttcca
     3301 cgggc
//
LOCUS       DATMVF010000185         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_122310, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000185 DATMVF010000000
VERSION     DATMVF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122310"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..148)
                     /locus_tag="VN178_09120"
     tRNA            complement(76..148)
                     /locus_tag="VN178_09120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:Arg,seq:cct)
     gene            548..1270
                     /locus_tag="VN178_09125"
     CDS             548..1270
                     /locus_tag="VN178_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWS81172.1"
                     /translation="MLLLIAGALGVGCGFGGGGDLTLGYLGWDENVANSNLTKVLLEE
                     ELGYENVELKLADGVGAVYRDLIEGETDAFLDSWMPNHEQFVEGGRGRIEVSKEPWYV
                     DPTRYGIAVPDYMQDVRSISDLDSSGTDMITGIEPGAVLMKKIETDVVPRYHLRSSLV
                     EATTPAMLAGLEQAYNMEEPFGFLALINAMRLDEDQVGSLELVINETKDPEKGVRRWL
                     EVKEYRELVRPWIEAAKNAQEA"
     gene            1273..2052
                     /locus_tag="VN178_09130"
     CDS             1273..2052
                     /locus_tag="VN178_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81173.1"
                     /translation="MTSKEQNTNDIRADMVEEAVRVAEEAESRDVALRLLGGVAIKVR
                     AHDGLEPAFEREYADLDWITQKGKSTQAQRFFESLGYVPQTRFNTMYGRERLLYFDEK
                     HDRQVDVLVGNFRMCHEIPFGDRMTMEPLTVPLAELLLTKLQIIELNEKDVGDALALL
                     HDHAVDEQDGDTINAAHIAKLCASDWGLWRTFTANLEVLDGYLGRFDLKDESRERITE
                     RVEALLARIEEESKTFGWKMRAKIGDRKRWYDLPEEVEGGP"
     gene            complement(2187..3635)
                     /locus_tag="VN178_09135"
     CDS             complement(2187..3635)
                     /locus_tag="VN178_09135"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWS81174.1"
                     /translation="MYTRTRDYSMYLAGEWVTGEARMEATSPATGEQIGTVPQGTRAD
                     VGRAIAAANEAWSGWANLSAFERGAAMIRIAEIIEERRDELAQTLTLDQGKPLVAEAY
                     DEVDELVAYFEMAGADVTRLEGAMPPSVDADKRVMLYRVPRGVVGVISPWNWPYTMPG
                     ELIAPALACGNAVVWAPASSTSICAVKLAECIVDAGLPSGLFNMITGPGPVVGDEVAA
                     NPGTQAVGFIGSTETGQKVAARAAGKPLILEMGGNGPVVILDDADLEAAAEASLTSAF
                     LGAGQSCTAGERFLVHERVYDAYLDRLGGAIERTIRLGDPFDPITTLGPLNNEPTAEK
                     MDLHISDALEGGAELVSGGARAEGFPTDLYYRPTVLSKVTAGMRVSREETFGPIVPAT
                     VIREEEEAIRVVNSSPYGLLCAVFTRDLARGLRFAEAVRTGWVNINASSNHWESHLPF
                     GGRAGSRSGVGRVGGRYAMESFTELKTVIVSL"
BASE COUNT          728 a         1158 c         1254 g          668 t
ORIGIN      
        1 taaatagagc ctcggcacca cgccggggct tctctaagtg tgacagattc cgcataccta
       61 taaagaattc ggaagcgcgc ctggaaggat tcgaacctcc gactcgcggt ttaggaaacc
      121 gctgctctat cccctgagct acaggcgcaa agcgccaaca gtttaacgcg cgatggccat
      181 gcttaccacc gggtcggggc cggggatctc ccggtccaca cactgtgcta gagtgggcca
      241 acacttcggg cgcatctggt gtcaggggcg cgtgtttcgg ttgaacgggg gtctggaacg
      301 acgtatgatt cagcggtact cgcgcccagg ttgggtgatt ggggtgcgag ggttgcatgc
      361 gtttttgttt ttggccgcgg gaggggtgtg agcggcgggg cgtgcgatcc tgggttgcgg
      421 gctgacgaag gagaggccgc ggaggctgaa ccacagttta gggagaggca gtcgtccgaa
      481 atgggcgaac ggtccgccga agagaacggg tccggtcggg ccgctcctga ctttgctgaa
      541 ggcctcagtg ctgttgctga tagcaggggc actcggtgtg ggctgcgggt ttggtggcgg
      601 cggggatctc acgctcggct acctgggctg ggacgagaac gtggccaact ccaacctgac
      661 gaaggtacta ctcgaagagg agctcggcta cgagaacgtc gagctcaagc tcgccgacgg
      721 cgtcggggcc gtctaccggg atctgatcga aggcgaaacc gatgctttcc tggattcctg
      781 gatgccgaac cacgagcagt tcgtggaggg agggcggggc cggatcgagg tctccaaaga
      841 gccgtggtac gtcgacccga ccaggtacgg gatcgccgtc cccgattaca tgcaagacgt
      901 gcgcagtatc tccgacctgg attcttcggg gaccgacatg atcacgggca tagagcccgg
      961 cgctgtgctg atgaagaaga tcgagaccga cgttgttccg cgttaccacc tgagatccag
     1021 cctcgtcgag gccacaacgc cggcgatgct tgccgggctc gagcaggcgt acaacatgga
     1081 agagccgttc gggttcctcg ccctcatcaa cgccatgagg ctcgacgagg atcaggtagg
     1141 cagcctcgag ttggtcataa acgagactaa agatccggag aagggcgtca gacggtggct
     1201 cgaagtgaaa gagtacaggg aactggtgcg gccatggatc gaagccgcaa agaacgcgca
     1261 ggaggcgtag taatgacgag caaagagcag aacacgaacg atatcagggc cgatatggtc
     1321 gaggaggccg tgcgggtcgc cgaggaggcc gagagtaggg acgtggcgct tcgcctgctc
     1381 ggaggggtgg ccataaaggt acgcgcgcac gacggtttgg agcccgcctt cgagcgggag
     1441 tacgccgacc tggattggat cacacagaag ggcaagtcta cccaggcgca gcgattcttc
     1501 gaatccctgg gatacgttcc gcagacgcgt ttcaacacta tgtacggccg ggagcgactg
     1561 ctctacttcg acgagaagca cgacaggcaa gtcgacgtcc tcgtcggcaa cttcaggatg
     1621 tgccacgaga tccccttcgg tgaccgtatg actatggagc cgcttaccgt cccactcgcc
     1681 gagctcctgc taacgaaact ccagatcatc gagctcaacg agaaggacgt cggcgacgcc
     1741 ctcgccctct tgcacgacca tgccgtggac gaacaggacg gagacacgat taacgctgcc
     1801 catatcgcca aactatgcgc ctccgattgg ggcctgtggc gcaccttcac ggccaacctc
     1861 gaggtccttg acggttacct ggggcgcttc gatctgaaag acgagagcag ggagcgtatc
     1921 acggagcgcg tcgaggcact gctcgcgcgc atcgaggaag agtcgaagac cttcgggtgg
     1981 aagatgcgcg cgaagatagg agatcgcaaa cgctggtacg acctgcccga ggaggtggaa
     2041 ggagggcctt gacagtgcgc cgtgcagaag gctcaagtgc cttctgcacg gcgcgctatc
     2101 agcaatcagc gatcagcaag aacaagaagc tgacggctga ccgctgaccg cgcgggttcc
     2161 ggaagttccc ttccggaacc cgctgactac agactcacga tgacggtctt gagttcggta
     2221 aaggactcca tcgcgtagcg tcctccgacg cgtccgacgc ccgagcggga acctgctcgt
     2281 ccgccgaagg gcaggtggct ctcccagtgg ttggatgagg cgtttatgtt gacccaaccg
     2341 gtcctcaccg cctcggcgaa gcgcaaaccc ctcgccagat cccgggtaaa gaccgcacag
     2401 agcaggccgt agggggagga gttgacgact cttatggctt cctcctcctc gcggatgacg
     2461 gtcgccggga ctatcggccc gaaggtctcc tccctagaga cgcgcatccc ggcggtgact
     2521 ttgctcaata cggtcgggcg ataatagaga tccgtaggga aaccctcggc gcgggccccg
     2581 ccggagacca attctgcgcc gccctcgagc gcatcggaga tgtgaagatc catcttctcg
     2641 gctgtcggtt cgttgtttag ggggcctagc gtggtgatcg ggtcgaaggg gtcgccgagt
     2701 ctgatcgtgc gctcgatcgc accgccgagc ctatcgaggt aggcgtcgta gaccctctcg
     2761 tgcaccagga accgttcccc ggcggtgcag ctctgaccgg cgcccaggaa ggcggaggtg
     2821 aggctcgcct ccgcggcggc ctcgaggtcg gcatcgtcca ggatcaccac cggcccgttg
     2881 ccccccatct cgagaattag cggcttgccg gcggcacgcg cggccacctt ctgaccggtc
     2941 tctgtagagc cgataaaacc gaccgcctgt gtacccgggt tggccgccac ctcgtccccg
     3001 acgacgggac cggggccggt gatcatgttg aagagcccgg agggcaggcc ggcgtcgacg
     3061 atgcactcgg cgagcttcac ggcacagatg gacgtcgagg aggcgggggc ccaaacgacc
     3121 gcgttgccgc aggcgagcgc aggcgcgatc agctcccccg gcatggtgta gggccagttc
     3181 cacggggaga tcacgccgac tacgccccgc ggaaccctgt agagcatgac gcgcttgtcg
     3241 gcgtctaccg agggcggcat ggccccctcc agccgcgtca cgtcggcgcc cgccatctcg
     3301 aagtaggcga cgagctcatc gacctcgtcg taagcctcgg ccacaagcgg cttgccctga
     3361 tccagggtca acgtctgtgc caactcgtcg cggcgttcct cgatgatctc cgcgatccgg
     3421 atcatcgctg ccccacgctc gaacgcggag agattcgccc accccgacca ggcctcgttc
     3481 gcggcggcta tcgcccgccc tacgtctgcg cgggtccctt ggggcacggt cccgatctgc
     3541 tctcccgtcg ccgggctcgt ggcctccatg cgggcctcgc cggtgaccca ctcaccggcg
     3601 aggtacatcg agtagtctct tgtcctggtg tacatcagat cccccttgcg aaggcgtcgt
     3661 ggtagatctc ctcgtagtct gcgacggtga cctcgcgggg gttcccgacc gtttgcgggt
     3721 cttcgtaggc gatccgggcc agcatcggga tctcgtcctc cgagaagccc aactcctgca
     3781 tcgtcggaat acctacgtcc tcggtcag
//
LOCUS       DATMVF010000186        11988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_122366, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000186 DATMVF010000000
VERSION     DATMVF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11988
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122366"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1575
                     /locus_tag="VN178_09140"
     CDS             109..1575
                     /locus_tag="VN178_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HWS81175.1"
                     /translation="MTTATERIEVAGYEVSPDHFIGGERVASERRFEDISPIDGEVMG
                     EVSRASEAEVDMAVRAAHDAFPDWADLGPAGRAPYLRRLADLIDENTGRLAAVENADM
                     AMLLRSLRARVISRGARNYRSYADLAVAHEERDWRSQGTWNRVQRMPAGPAAVITPWN
                     APFMLSTWKTAPALAAGCTVVLKPAEWSPLSCSLLADLFAEADFPPGVFNVVQGMGEE
                     AGAALVAHPLLRRVSFTGSPETGRLIGVSAAKNLVPFTAELGGKGPLVVFEDADLEAA
                     AAKAAGQYDDGGQVCLAGTRILVQESVREPFLERFHAAVDEHVLGDPREDETTVSPLI
                     HPDHLARVEGFVDRAREGGQNIVRGGRRSDAGPLFYEPTLIEPTSNDAEVVQNEVFGP
                     VLTFQTFADEDEAVALANSTPYGLSAIVYTGSMGRADRVGRRIRAGVVWVNTFLIRDL
                     TAPFGGMGISGIGREGGDYALDFYSDLKTLQILDGSVR"
     gene            1572..2879
                     /locus_tag="VN178_09145"
     CDS             1572..2879
                     /locus_tag="VN178_09145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HWS81176.1"
                     /translation="MRTGATPGETASDEAILDAVAAEAGWMEDLLVRLVEATTTLGDE
                     EAGQRIMEQAFADCGLELRNVYLDAEALRNAEGASPFSWDVRGKRNVVADWAARGSGG
                     RSLILNGHVDVVPPAAEALWTNPPFRAVRDGDWLYGRGAGDMKAGLTAMTGAVRALKR
                     AGYAPAADIQLQSVVEEECTGHGALQCLLDGTRADACVISEPHPDHITVAQVGVLWFH
                     VELAGVPAHAARASHLGFNAIDAAVEVLAALRRHEARLNENPPPPFDAFEHPINLNPG
                     VISGGDWPSTVAATCTLSCRIGLYPGQEPEEARAGVEAAVAEAVAASPRLAENPPRVR
                     YDGFSCEGSVIGVNEPLVQTLSGAYARLHGGCPPALATTATTDARHFVRMGMPAVCFG
                     PRGEGIHGIDERVSLRSVVESAQVLGLFVRDWCGLVPGRKEES"
     gene            2879..4399
                     /locus_tag="VN178_09150"
     CDS             2879..4399
                     /locus_tag="VN178_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWS81177.1"
                     /translation="MSTAEPSITPRPGTTESSKLHRRALGVPDLMFFIIAASAPLTAV
                     AGGQAATYLVTGNRAVAFMFIPLGIVLALFTVGYAAMSRYIENAGAFYAYVSKGLGRV
                     SGIGAAFVALIAYNSMQIGIYGLFGVAMGGFMAGYLGLDLPWWFWCLVAGGVIALLGL
                     LQIDLNAKVLAVMLGLEVLIVALFDFAILADPGPQGLTMIGFSPTIAFSTAVGATLTF
                     CVASFVGFESAAIYSEECRDPRRTVARATYLAVGLIAVFYALSSWLLGVAAGPNTIVN
                     PEALVRAGFETDGAPDPTTVLFITGAERLGEIWGTAAALLFATSLFAALLSFHNAVAR
                     YAFALGREGVLPSVFGRVNSRTGAPWVASLTQSILALSVVLVFAIAAANPVLTLFTWL
                     TNLGALGVLLLMASTSFAVVGYFRRRPQDAVGSWAATWAPAIAGVLLLILLVLGVANF
                     NVLITGLTDAPINTMTIVLPLILFGGGIVGLIVGAFLKRSRPDVYERIGGGAGAEE"
     gene            4414..5868
                     /locus_tag="VN178_09155"
     CDS             4414..5868
                     /locus_tag="VN178_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWS81178.1"
                     /translation="MGSQRDRTRMNAAPQHEQADLPALADREGILGDLERFICEAWES
                     FDTPRTAEPQLDETLIGRLETPLPEEAGDAEATLKDAARILDASISPSRPLYVGYIGS
                     TGLEIGVLASALAATYDANLAVAAGGADLLEEQTLRWLAAFVGFPYAEGVFTSGGMTS
                     NLTALLAARERALPGCRTDGLGDHRTAVYCSDEAHHSVVRAVEVCGLGSSAIRQIPID
                     GRRRMRPEALDEALSKDVALGVVPVAVVATAGTTLTGAVDPLDAVADVCARHDVWLHI
                     DGAYGLPGAAAPTAAPLFAGLERADSATIDAHKWLGVQKSCSVVLLSERDRLRAAFGH
                     EERYMLHEGDVANPVDRTLEYSRPFRSLRLWMAFRVYGAARYRAWIEATLANARHLTE
                     SLRDSPEFELLHDPMLSTVCFQHVPPGADDVDAHNIKLAREMQRDGRVFLAPATVDGH
                     ACLRVCFVNFRTTSDEVEFVLTVAQELGRRLVLG"
     gene            5999..6238
                     /locus_tag="VN178_09160"
     CDS             5999..6238
                     /locus_tag="VN178_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81179.1"
                     /translation="MPRIQPLEPENAPEQSRQLMEQGEQAMGQMLNFFKQMAVSPAAF
                     QAYMDFEEALQGGALDQLTREAVYIGTSDYNGCTY"
     gene            6275..6544
                     /locus_tag="VN178_09165"
     CDS             6275..6544
                     /locus_tag="VN178_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81180.1"
                     /translation="MSKDEIERAQRFESEDEQRAAALRFAREVVESRGRPSDEAMEEV
                     RNAGYTDEQIIEMVATVNLATFTNYMNDFMGTEVDLPVQEPTDDR"
     gene            6551..6952
                     /locus_tag="VN178_09170"
     CDS             6551..6952
                     /locus_tag="VN178_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS81181.1"
                     /translation="MRGGCAASLASNGEHAIELARELQPDVTLVDIEMPGINGIEATR
                     EILHLSPRVGVLVVTMFEDDSVFAAMRAGTRGYLLKDSRGKEVRHAIRAVASGEAIFG
                     PGSSVPGRPGWGTKEPSRDTLYRSGSCAPDV"
     gene            6997..8091
                     /gene="tal"
                     /locus_tag="VN178_09175"
     CDS             6997..8091
                     /gene="tal"
                     /locus_tag="VN178_09175"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HWS81182.1"
                     /translation="MNERLEKMRELGQAPWVDELAREDIKNGGLERMIEDGIVGVTSN
                     PAIFQKAIAGSDLYDEQLHELAQTEDDPKRIFWEVARKDISDACDIFMPVYEETGGRD
                     GFISLEVQPDIAYDTEATVEEASRLHEMVDRPNLFVKIPATLPGLVAIEEMISRGKSI
                     NVTLIFSLERYREVARAYTRGLQRLVENGGDPSGVHSVASFFVSRIDTEADRQLEEVG
                     AEDLRGRLAIANAKLAYQIYKQVFGGARWRKLAENGANTQRLLWASTSTKNPDYPDTI
                     YIDNLVGPNTVNTMPKKTIEAVIDHGDISPTLEEGVEEAKRLLKRLREAGLDYEDVTD
                     ILEKEGIQKFADPFNEMLQEIENKSRQLVS"
     gene            8318..9235
                     /gene="gnd"
                     /locus_tag="VN178_09180"
     CDS             8318..9235
                     /gene="gnd"
                     /locus_tag="VN178_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWS81183.1"
                     /translation="MDIGIWGMGRMGFNMARRLVDKGDERVIAGNRSPGKVDEAVEHG
                     AEGAYSVEEFVGMQESPRVLWTMLPAGDVTDDMIHQFMELADEGDIIVDGANSYFRDS
                     VARAREVRNAGLRWLDAGVSGGVWGYDVGYCTMIGGDADAFEYAEPAFQTLAPPNGYA
                     YLGDAGAGHFAKMVHNGVEYGMLQAYAEGFEILQKSRYDYDLRALSSLWNQGSVVRSW
                     LLELAESAFEKDANLDSISGYVEDSGEGRWTVLEAIEEDVPANAIAGSLFARFASRQD
                     DSFAMKVIAALRGEFGGHAIKEAATEQEK"
     gene            9243..10778
                     /gene="zwf"
                     /locus_tag="VN178_09185"
     CDS             9243..10778
                     /gene="zwf"
                     /locus_tag="VN178_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWS81184.1"
                     /translation="MADTQVMQNILRDEQRLPRVPEPGVMVIFGASGDLTSRKLVPAL
                     YELAAQRRLPLEFAVIGISRTEMSHEEFRGRLRKTLEEQRSGEVSDDVWESFSNGIFY
                     MPGDSKKTETYGELKELLKRLDSERGTQGNRAFYLSSSPSLFPAIVENLGEAGLNRGE
                     DGGWSRLVVEKPFGRDLGSARELNVELQRYFDETQIYRIDHYLGKETVQNILALRFAN
                     GIFEPVWNQHYVDHVQITVAEDIGVGSRGAFYEEAGAMRDIVQNHVMQVLCLTAMEPP
                     VTFDAESVREEKVKVLRAVRPIPEHEVEDYAVRGQYERGWVWGEEVPGYREEKNVDPE
                     SVTETFVALKVFVDNWRWARVPFYIRAGKRLPKKATEVAIQFHSSPHTPFARDDTEGL
                     EPNVLVIRVQPEEGLSLKIGAKVPGTGFEVSSVNMDLLYGTAFLEEAPDAYQRLLLDL
                     MLGDPTLFIRADEAEGAWRILDPVMRHWSQKREVSFYPAGTWGPEEADALLERDGREW
                     RRP"
     gene            10775..11842
                     /locus_tag="VN178_09190"
     CDS             10775..11842
                     /locus_tag="VN178_09190"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HWS81185.1"
                     /translation="MIGETRQAPGGARMNVGQIERELGKLRTNDDGTLALRSSVLNLI
                     VVTDEESAQKITGSVSKLAGRHPARAVVLIADPEGEPNVDVGLSAFCNVRGGSSAQVC
                     AEQVTIHAEGPAARHLESFAGPLLIPDLPVFLWYPGRFSPRSQEFAAMADLADRVIVD
                     SAASQNHETCLREIAALIGDPEMPAIGDLQWVGLSPWRSLLADTFGTPERAGDLEKIE
                     SAEILYAPGGENRALLLLGWLASTLGWRPECRNGGGQNIEFSGPSGRIVVVLSPDSPD
                     ARLRRIRLYSEEYSFQVSRHRELSHARTTVMRGDELLAERTVHLGSFDLGVLVGEELR
                     YRGRDEAYERSLLEAVEILDL"
BASE COUNT         2167 a         3846 c         3976 g         1999 t
ORIGIN      
        1 agccccaggc gaggagcacg acaccctggc cccggtcgaa atagaccgtg gctaccgctt
       61 taccttcggc tatacggtag acgacctcga gactgtgaag gagctcgtat gaccaccgcc
      121 acagagcgca tcgaggtcgc aggctacgag gtctctcccg accattttat cggcggcgaa
      181 cgcgtcgcga gcgaacggcg cttcgaggac atctcaccca tcgacggcga ggtcatgggc
      241 gaggtctcgc gcgcctccga ggctgaggtc gacatggcgg tgcgcgccgc gcacgatgct
      301 ttccccgatt gggcggacct cggtccagcc ggacgcgcac cgtatctgcg ccgcctcgca
      361 gacctcatag acgagaacac gggcaggctc gcggccgtcg agaacgccga catggccatg
      421 ctgttgcggt cgttgcgcgc ccgtgtgatc tcgcgcgggg cgcgcaacta ccggtcgtac
      481 gccgacctcg ccgtcgccca cgaggagcgc gactggcgtt cgcagggtac gtggaaccgc
      541 gtgcagcgca tgcccgcggg gcctgcggcc gtgatcacac cgtggaacgc cccgtttatg
      601 ctctccacct ggaagaccgc gccggccctc gccgccggct gcaccgtagt cctcaagcca
      661 gcagagtggt ccccgctctc ttgctcgctc ctggccgacc ttttcgcaga agcagacttc
      721 ccccctggcg tcttcaacgt ggtccagggg atgggggagg aggccggcgc ggcgctcgtc
      781 gcgcatcctt tgttgcggcg ggtctctttc accggctcgc ccgagacggg gcgtctgatc
      841 ggggtctccg ctgccaagaa cctggttccg ttcaccgccg aactaggcgg caagggcccc
      901 ctcgtagtct tcgaggacgc cgacctcgaa gccgcggccg caaaagcggc tggccagtac
      961 gatgacggcg gccaggtgtg cctggccggg accaggattc tcgtccagga gtcggttcgc
     1021 gagcccttcc tcgaacgttt ccacgccgcg gtcgacgagc acgtactcgg agatcctcgc
     1081 gaggacgaga cgacggtctc gcccctgatc caccccgacc acctcgcgcg cgtggagggc
     1141 ttcgtcgatc gggcccgcga gggcgggcag aacatagtac gcggtggacg ccgctctgac
     1201 gcggggccgc tgttctacga gccgaccctc atagagccga cctccaacga cgccgaggtg
     1261 gtgcagaacg aggtcttcgg tcccgtcctc accttccaga cctttgccga cgaggacgag
     1321 gcggtcgccc tcgccaactc cacgccctac ggcctctccg ccatcgtgta cacgggatcg
     1381 atgggccgcg ccgaccgcgt cggacgccgg atacgcgccg gcgtggtctg ggtcaacacg
     1441 ttcctcatcc gcgacctgac ggcgcccttc gggggcatgg gtatctccgg catagggcgc
     1501 gagggcggag actacgccct ggacttctac tcagacctga agacgctcca gatcctcgac
     1561 ggtagcgtga gatgagaacc ggcgccacgc caggcgagac cgcctccgat gaggctatcc
     1621 tcgacgcggt cgccgccgag gccggctgga tggaggacct cctcgtccgc ctcgtcgaag
     1681 cgaccacgac cctcggggac gaagaggccg gacagcggat catggaacag gccttcgccg
     1741 actgcggtct cgaactacgc aacgtctacc tggacgcgga agcactgcgc aacgcggagg
     1801 gcgcctcacc gttttcgtgg gatgtgaggg gcaagcgcaa cgtcgtcgcc gactgggccg
     1861 cgcgtggcag cgggggacgt tcgctcatcc tgaacggcca cgtggacgtc gtgccgccgg
     1921 ccgcggaggc gttgtggacc aacccgccgt tccgggcggt gcgtgacggg gattggctct
     1981 acggccgtgg cgcgggggac atgaaggccg gcctcaccgc catgaccggc gcggtgcggg
     2041 cgctaaagag ggcaggttac gcccctgcgg cggacataca gctgcagtcc gtcgtcgagg
     2101 aggagtgcac ggggcacggc gcgttgcagt gtttgctgga tggtacgcgg gccgacgctt
     2161 gcgtcattag cgagccgcat ccggaccata ttaccgtcgc ccaggtcggc gtgttgtggt
     2221 tccacgttga gctcgccggc gtcccggcgc acgcggcgcg cgccagccat ctcggcttca
     2281 acgccatcga cgccgcggtg gaggtgctgg cggcgctccg gcgccacgag gcgcggctca
     2341 acgagaaccc gccgccgccg ttcgacgcgt tcgagcaccc catcaacttg aaccctggtg
     2401 tgatctcagg cggtgactgg ccctctaccg tagcggcgac gtgtacgctc tcttgccgca
     2461 tcgggctgta cccggggcag gagcccgagg aggcgcgcgc cggggtcgag gccgccgtgg
     2521 ccgaagccgt cgccgcatcc ccacggctgg ccgagaaccc gccgcgcgtg cgctacgacg
     2581 ggttctcgtg cgagggctcg gtcataggtg tgaatgagcc gctggtccag accctgtcgg
     2641 gggcgtacgc ccggttgcac ggagggtgtc ctccggcgct ggcgacgacg gccaccacgg
     2701 atgcgcgcca tttcgtacgc atggggatgc ccgctgtttg cttcgggccg cggggggagg
     2761 ggatccacgg catcgacgag cgcgtctcgc tgcgctccgt cgtcgagtcc gcgcaggtgc
     2821 tcgggctctt tgtaagggat tggtgcgggc tcgtcccggg ccggaaggag gagagctgat
     2881 gtccacagcc gaaccgagca tcaccccgcg accagggacc accgagagct ccaaactgca
     2941 ccggcgcgcc ctgggggtgc cggatctgat gttcttcatc atcgcggctt cggcgccgct
     3001 tacggcggtg gccggcggcc aggccgcgac ctacctcgtg accggcaaca gggccgtcgc
     3061 tttcatgttc atacccctcg ggatcgtgct cgcgctgttc accgtcgggt acgcggcgat
     3121 gagccgttac atcgagaacg cgggggcgtt ctacgcctac gtctccaagg gcctcggccg
     3181 ggtctccggc atcggggccg cgttcgtcgc gctcatcgcc tacaacagca tgcagatcgg
     3241 gatctacggc ctcttcgggg tcgcgatggg cggctttatg gcgggatacc tgggcttgga
     3301 ccttccctgg tggttctggt gcctcgtggc agggggcgtg atagcgctgc tcggattgtt
     3361 gcagatcgac ctaaacgcca aggtgctggc cgtcatgcta ggcctggagg tgctgatagt
     3421 cgccctcttc gacttcgcga tcctggccga ccctggtcca cagggcctga cgatgatcgg
     3481 cttcagcccg accatagcgt tcagcaccgc cgtcggcgca acgctcacgt tctgcgtcgc
     3541 ctctttcgtg ggcttcgaat cggcggccat atacagtgag gaatgccgcg accccaggcg
     3601 gacggtcgcc cgcgcgacct acctggccgt gggcctgatc gccgtgttct acgccctctc
     3661 cagctggctg ctcggcgtcg cggccggacc gaataccata gtcaaccccg aagccctcgt
     3721 tagggccggg ttcgagaccg acggcgcccc tgatcctacg accgtgctgt tcatcacagg
     3781 ggccgagcgg ctgggtgaga tctggggcac tgcagcggcg ctgctcttcg ccacgagcct
     3841 tttcgccgcg ctcctctcgt tccacaacgc tgtcgcccgc tacgcattcg ccctgggccg
     3901 tgagggagtg ctcccttcgg tcttcggccg cgtcaacagc cgcaccggcg cgccctgggt
     3961 cgcctcgctg acgcagtcta ttctcgcgct cagtgtcgtc ctggtcttcg ccatagccgc
     4021 ggccaacccg gtgctcacgc tgtttacgtg gttgacgaac ctcggcgctc tgggcgtgct
     4081 actcctgatg gcttcgacgt ccttcgcagt cgtagggtac ttcaggcgga ggccgcagga
     4141 cgcggtcgga agctgggccg ccacgtgggc gccggcgatc gccggcgttc tgctcctgat
     4201 cctgctcgtg ctcggcgtcg ccaacttcaa cgtcctgatc acgggcctca ccgacgcccc
     4261 catcaacacg atgacgatcg tgctccccct catcctcttc gggggtggga tcgtcggcct
     4321 gatcgtgggc gccttcctga agcgtagccg ccccgacgtc tacgagcgca tcggcggcgg
     4381 cgctggcgcc gaggaatagc ggcggcagga gacgtgggtt cccagaggga cagaacgagg
     4441 atgaacgccg cgccgcaaca cgagcaggcc gacctccccg cgctcgccga ccgcgaaggg
     4501 attctgggag atctcgagcg tttcatctgc gaggcgtggg agtcgttcga cacaccgcgc
     4561 accgccgaac cgcaattgga cgagactctg atcggacgcc tcgagacgcc gctgcccgag
     4621 gaggccggtg acgccgaggc cacactgaag gacgcggcgc gtatcctcga cgccagcatc
     4681 tcgccctcca gaccgctcta tgtcggctat attggctcga ccggcctgga gataggggtg
     4741 ctagcctccg cgctcgcggc gacctacgat gctaacctgg cggtcgcagc aggcggcgcg
     4801 gatctcctcg aggagcagac cctacgctgg ctcgccgcgt tcgtcggctt cccttacgcg
     4861 gaaggcgtct tcacgagcgg cggaatgacc tcgaatctta cggcgctctt ggcggcgcgc
     4921 gagcgggcgt tgccagggtg ccgcaccgac ggactcgggg accaccggac ggcggtgtac
     4981 tgctctgatg aggcccacca ctcggtcgtt cgcgccgtag aggtctgcgg gctcgggagc
     5041 agcgccatcc ggcagatccc catcgacggc cggcgacgaa tgcggcctga ggcacttgac
     5101 gaggcgctct ccaaggacgt cgccttgggc gtcgtgcccg tagcggtggt tgcgacggcg
     5161 ggaacgaccc ttacgggggc cgtcgacccg ctcgacgcgg tcgccgacgt ctgcgcccgc
     5221 cacgacgtct ggttgcacat cgacggcgcg tacgggcttc ctggagcggc ggcgccaacc
     5281 gcggcaccgc tgttcgctgg cctcgagcgt gccgactctg ccaccatcga cgcccacaag
     5341 tggctcggtg tgcagaagag ctgcagcgtc gtgttgctga gtgagaggga cagactgcgg
     5401 gcggcgttcg gccacgagga gcgctacatg ctgcacgagg gcgacgtagc caacccggta
     5461 gaccgtacgc tcgagtactc gcgtccgttc cgctctctgc ggctgtggat ggccttccgc
     5521 gtctacggcg ccgccaggta ccgcgcctgg atcgaagcca ccctcgccaa cgcccgacat
     5581 ctcacggagt cgctgcgcga ctcaccagag ttcgagctcc tccacgaccc catgctctcc
     5641 accgtctgct ttcaacacgt tccacccggc gcagatgatg tcgacgcgca caacatcaaa
     5701 ctcgcccgcg agatgcaacg cgacgggcgc gtgttcctcg cgcctgccac cgtcgacggc
     5761 cacgcctgcc tgcgcgtgtg cttcgtcaac ttcaggacca cgtccgacga ggtagagttc
     5821 gtgctcacgg tggcccaaga gctcggtcgt cgcctggtcc tgggttgacc cggggccacg
     5881 gccagcattt cagcagttcg actagcattg gaccaccttt tcccctcctc tttttcgcac
     5941 ctgccgaaac gggtaggaaa gggttgggca gatcgtggca aacccaaagg aggatcttat
     6001 gccgcgtatc caaccactgg aaccggagaa cgccccggag caatcacggc agttgatgga
     6061 gcagggcgag caggccatgg ggcagatgct gaacttcttc aagcagatgg ccgtctcccc
     6121 ggcagccttc caagcgtaca tggacttcga ggaggcgcta cagggtggcg ccctcgacca
     6181 gttgacccgt gaggccgtct acataggaac ctccgactac aacggctgca cctactgact
     6241 gtcggcccac tccgccgtgg cggagcaagc gggcatgagt aaagacgaga tcgagcgcgc
     6301 gcagcggttc gagtctgagg atgagcagcg ggcagcggcc ctccggttcg cccgggaggt
     6361 ggtcgagagc cgcgggcgcc cttcggacga ggctatggag gaggtgcgaa acgccggtta
     6421 caccgacgag cagatcatag agatggttgc caccgtgaac ctcgctacct tcaccaacta
     6481 catgaacgac tttatgggaa ccgaggtgga ccttccggtc caggagccga cggacgaccg
     6541 gtaggacctt ttgcggggcg gctgcgccgc atctttggcc agcaacggcg agcatgccat
     6601 agagctggct agggagctac agccggatgt gacccttgtg gacatcgaga tgccaggcat
     6661 taacggtata gaggctacgc gtgagatcct gcacctaagc ccccgcgtcg gcgtactggt
     6721 agtcacgatg ttcgaggacg actcggtgtt cgccgccatg cgagccggga cgagaggtta
     6781 cctgctaaag gactcgaggg gtaaggaggt gaggcacgcg ataagggccg tggccagcgg
     6841 cgaggcgatc ttcggccccg ggtcatccgt gcccgggagg ccgggttggg gcacgaaaga
     6901 acctagccga gataccctct atcgaagcgg ctcctgtgcc cctgacgtgt gaggtagact
     6961 gaaagaacag gacgcgctac cgtcaggagg actctggtga acgagcgttt ggagaagatg
     7021 cgggagctgg ggcaggcgcc ctgggtcgac gagctcgcgc gcgaggacat aaagaacggc
     7081 ggcctcgagc ggatgataga agatggcatc gtgggcgtta cctctaaccc ggccatcttc
     7141 cagaaggcca tagcgggctc ggatctctac gacgagcaat tacacgaact ggcccaaaca
     7201 gaagacgacc cgaagcggat cttctgggag gtcgccagga aggacataag cgacgcctgc
     7261 gacatcttta tgcccgtcta cgaggagacc ggaggacggg atggctttat ctcccttgag
     7321 gtccagcccg acatagccta cgacacggag gccacggtcg aggaggcctc ccgcctgcac
     7381 gagatggtcg acaggcccaa cctcttcgtc aagataccgg ccaccttgcc cgggctcgtc
     7441 gccatagagg agatgatctc ccgcggcaag tccataaatg taaccctgat cttctccctc
     7501 gagcgctacc gcgaagtggc ccgcgcctac acgagaggcc tccagagact ggtcgagaac
     7561 ggcggagacc cctccggcgt gcactccgtg gcgagcttct tcgtctccag aatagacacc
     7621 gaagcggata gacaactaga agaggtcggc gccgaagacc tgagaggcag actcgccata
     7681 gcgaacgcca agctggctta ccagatctac aaacaagtct tcggaggcgc cagatggcgc
     7741 aagctagctg aaaatggcgc caacacccag cgcctcctct gggcctcgac ctcgaccaag
     7801 aaccccgact accccgacac catctacatc gacaacctcg tcggacccaa caccgtcaac
     7861 accatgccca agaagaccat agaggccgtc atagaccacg gcgacataag ccccaccctt
     7921 gaagaaggcg tcgaagaggc caagagactc ctgaagcgcc tgagagaagc cggcctcgac
     7981 tacgaggacg taaccgacat cctggagaag gaaggcatcc agaagttcgc cgatccgttc
     8041 aacgagatgc tgcaagagat cgaaaacaag agccgccaac tagtgagcta gtcagcacgc
     8101 ctgcttcgca ggcttgtcag cacttcagct agtcagcttg cgatcggacg gcgaggagat
     8161 tacttgttca aagacacgat tcgtgaagag agtgattcgc ggacgagcac gggctgcgaa
     8221 ccgacataaa gagctgaaat gctgactagc tgacaagcga aggcctgaaa ggcctgagcg
     8281 tgctgactag ctagcgacga ccgaagggag gagcgaagtg gacatcggta tctggggcat
     8341 ggggcggatg ggcttcaaca tggcacgccg actcgttgat aagggcgacg agcgtgtgat
     8401 cgcgggcaac cgttcgcccg gcaaggtgga cgaggcggtc gagcatgggg ccgaaggcgc
     8461 ctacagcgtg gaggagtttg tagggatgca ggagtctccg agggtgcttt ggaccatgct
     8521 ccctgccggg gatgtgacgg acgatatgat ccatcagttc atggagcttg ccgacgaggg
     8581 cgacatcatc gtggacggtg ccaactccta cttcagggac tccgtggcga gggccaggga
     8641 ggtcaggaac gcgggcttga ggtggctcga tgccggcgtg tcggggggcg tctggggcta
     8701 cgacgtcggc tactgcacca tgataggggg cgacgccgac gccttcgagt acgccgagcc
     8761 tgctttccaa accctggcac ccccgaacgg atacgcttat ctcggggacg cgggtgccgg
     8821 ccacttcgcc aagatggtcc acaacggggt cgagtacggg atgctccagg cttacgccga
     8881 agggttcgag atcctccaga aatcccgcta cgactacgac ctcagggccc tctccagcct
     8941 gtggaaccag ggaagcgtcg tccgtagctg gctcctggag ctcgccgaga gcgccttcga
     9001 gaaggacgcc aacctcgaca gcatcagcgg ttacgtcgag gactccggcg aggggcgctg
     9061 gaccgtgctc gaggcgatag aagaagacgt accggcgaat gccatagcag gctccctctt
     9121 cgcccgcttc gcctcccgcc aggacgattc cttcgccatg aaggtcattg ccgccctgcg
     9181 cggcgagttc ggcgggcacg cgatcaaaga ggctgcgact gaacaagaga agtagccaag
     9241 cgatggccga tacgcaggtt atgcagaaca tcctcaggga cgagcagagg ctccctcgtg
     9301 tgcccgagcc tggtgtgatg gtgatcttcg gggcctctgg agaccttaca tcccgcaagc
     9361 tggttccggc tctctatgag ctcgctgcgc agaggagatt gccgctcgag ttcgcggtca
     9421 ttggtatctc gcggaccgag atgagccatg aggagtttcg gggcaggctc cgaaagacat
     9481 tggaggaaca gcgctccggg gaggtctctg acgacgtctg ggaatccttc tcgaacggta
     9541 tcttctacat gcccggcgac tcgaagaaga ctgagaccta cggcgaactc aaagaactat
     9601 tgaagaggtt ggatagtgag cggggaaccc aagggaacag ggccttctac ctctcttctt
     9661 cgccttcgct cttcccggcc atagtggaga acctgggcga ggccggcctg aacagagggg
     9721 aagacggcgg ctggtcgagg ctggtcgtag agaagccttt cgggcgggac cttgggagtg
     9781 ccagggagct caacgtagag ctccaacggt acttcgatga gacccagatc taccgcatcg
     9841 accactacct cggcaaggag acggtccaga acatcctcgc cctgaggttc gccaacggga
     9901 tcttcgagcc ggtctggaac cagcactacg tcgaccacgt ccagatcacg gtcgccgaag
     9961 acataggcgt gggcagccgc ggggcgttct acgaggaagc cggcgccatg cgcgacatcg
    10021 tccagaacca cgtcatgcaa gtcctctgcc ttacggcgat ggaaccgcct gtgactttcg
    10081 acgccgagtc agtgcgcgag gagaaggtaa aggtcttgag agccgtccgt cccatccccg
    10141 agcacgaggt cgaggactac gcggtgcgcg ggcagtacga gaggggctgg gtgtggggtg
    10201 aagaggtgcc aggctatcgc gaggagaaga acgtcgaccc cgaatccgtg accgagacct
    10261 tcgtcgcgct caaagtattc gtggacaact ggcggtgggc gagggtcccg ttctacatca
    10321 gggccggcaa gaggcttccg aagaaggcga cggaggttgc catccagttc cactcctcgc
    10381 cgcatacccc cttcgcccgc gacgacaccg agggcctcga gccgaacgta ctcgtcattc
    10441 gtgtccaacc cgaagaaggt ctctccctga agatcggggc caaagtccct ggcaccggct
    10501 tcgaggtcag ctcggtgaac atggacctgc tctacggtac ggccttcctc gaagaagctc
    10561 cggatgccta ccagcggctg ctcctcgacc tgatgctcgg cgacccgacg ctgttcatcc
    10621 gggccgacga ggccgagggt gcctggagga tactcgaccc cgtgatgagg cactggtcac
    10681 agaagcggga ggtatcgttc tatcctgcag ggacctgggg accggaggag gccgacgcgc
    10741 ttctcgagcg ggacggaagg gagtggagga ggccatgatc ggggagacgc gccaggcccc
    10801 cggtggcgcg aggatgaacg tcgggcagat agaacgcgag cttggcaaac tgcgcaccaa
    10861 cgatgatggg acgctcgccc tgcgctccag cgtgctcaac ctgatcgtgg tgaccgacga
    10921 ggagtcggcg caaaagatca ccggctcggt ctcgaagctg gcgggccgcc atccggctcg
    10981 ggccgtggta ctgatcgccg accccgaagg cgaaccgaac gtggacgtgg ggctctccgc
    11041 cttctgcaac gtcaggggtg gcagcagcgc acaggtctgc gccgagcagg tgaccataca
    11101 cgccgaaggt cctgcggcga ggcacctgga gagcttcgcc ggtccgctcc ttatccccga
    11161 cctgcccgtg ttcctgtggt acccgggcag gttctcgccc cgctcgcagg agttcgcggc
    11221 gatggccgat ctcgcggacc gagtcatcgt ggactcggcg gcctcgcaga atcacgaaac
    11281 ctgcctgcgc gagatagcag ccctgatcgg agatcccgag atgccggcca tcggggacct
    11341 gcagtgggtc gggctctcgc cctggcgctc gctcttggcc gataccttcg gcaccccaga
    11401 acgggcggga gacctcgaaa agatcgaaag cgcagagatt ctctacgcgc cgggcgggga
    11461 gaacagggca ctgcttctcc tgggctggct cgcctccacc ctgggatgga gacccgagtg
    11521 ccgtaatggt ggcggccaga atatagagtt ctccgggccc tcgggtcgga tcgtcgtcgt
    11581 gctctctcct gattcacccg acgccaggct gaggcgcatc cggctgtact cggaggaata
    11641 cagcttccag gtctcgcgcc accgtgagct ctcccacgcg aggactaccg tgatgcgggg
    11701 agacgaactg ctcgccgagc gcaccgtcca cctcgggtcc ttcgatctcg gggtcctggt
    11761 cggggaagag ctgcgctatc gcgggcgcga cgaagcctat gaacgatctc tgctcgaggc
    11821 cgtggagata ctcgacttgt gagcgtccac gtctatagta gtcaagaaga gctctccgcg
    11881 gccgccgcgc gcgagttcgc ggccagggcc ggggaggcaa tcgaggagca cgggcgtttc
    11941 gccgtggtgc tcgcgggcgg ctcgacgccg gagaccatgt acgcgatc
//
LOCUS       DATMVF010000187         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_398637, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000187 DATMVF010000000
VERSION     DATMVF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398637"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VN178_09195"
     CDS             complement(<1..239)
                     /locus_tag="VN178_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81186.1"
                     /translation="MVQKEELVARPPELSDESVTCVRVLYPDLHGVARGKDVPIGEFE
                     RAIEHGLAFCSAVMGTDLRHTPVVGGEEGYPDLIA"
     gene            complement(242..955)
                     /locus_tag="VN178_09200"
     CDS             complement(242..955)
                     /locus_tag="VN178_09200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HWS81187.1"
                     /translation="MKPVLIRQHEEMTPPGLLVEWLEDRGISYEVNYSYKDGSIPDPS
                     DYSFVASLGSPYGPNDTHEPGVLAELELVGAAVEKDVPVLGLCFGGEVLSAVLGGRVE
                     RAPVPELGWREIETDDPGAIPSGPWLEWHYERFTTPPGAVEVARTADAVQAFRLGPHL
                     GVQFHPESTVEIVADWAGADTENLAKLGIGDGRDLLAIPPEREEAAREAAFRLFDAFL
                     AEAATHRSENSAGAAGGKE"
     gene            complement(952..1806)
                     /locus_tag="VN178_09205"
     CDS             complement(952..1806)
                     /locus_tag="VN178_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWS81188.1"
                     /translation="MGRRVRILHEGGSAWGRLEGGEVVLEGGGRVPEAEAEYLAPAEP
                     TKILAVHLTYRSRVDEYAARTPPQPSYFLKPTTTLNGHRGVIRRPRGTEFLNYEGELA
                     VIVGHRMKGVAEEDALSYVGGYTCANDVGLHDFRHADRGSMLRVKGQDGFLPLGPEVV
                     SADEFDPEDFRLRTYINGEVVQEGGSDDLIFPVAYQLADLCRLITLEPGDVVLTGTPA
                     NSRPMEPGDVVEVEIDGIGRLTNTVEEWDVDLTGPGEQPETSANTLHVALAVSEEEAE
                     KMVSEAGG"
     gene            1908..2600
                     /locus_tag="VN178_09210"
     CDS             1908..2600
                     /locus_tag="VN178_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWS81189.1"
                     /translation="MESVPRSTAASDPIRVDSVADLAYERIRGYVLGGEVPPGARLGQ
                     VELAERFGISRTPVREALRRLAGEGLVDFHSNRGFRVADLGLDAVLRRMEVRSIVEPG
                     IARLAAQRRTEEDLQRLEEAIASEEGARDGISAHDASRDFHIALALASGNEELMRVLN
                     SLWLVEVGRRLLSRRTADPNWLHDDVSEHREILTAVSEGRAADAERLMAEHIARAVHH
                     WEPLGSGGTRDG"
     gene            complement(3061..3315)
                     /locus_tag="VN178_09215"
     CDS             complement(3061..3315)
                     /locus_tag="VN178_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81190.1"
                     /translation="MRSKVCRGCRVEKNLSEFYKHPQMTDGHLNKCKVCIRARTQKYR
                     REHLEQYAEYEKARVNLPHRVEVRRKYEEEHKEQIAEYKK"
     gene            complement(3317..3799)
                     /gene="ssb"
                     /locus_tag="VN178_09220"
     CDS             complement(3317..3799)
                     /gene="ssb"
                     /locus_tag="VN178_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWS81191.1"
                     /translation="MVSFNRVVLAGNLTRDPELRFTNDGIPVCSFGLAVNRVRSRSEE
                     VDFFNITAWRELGETVANYKKKGDPILLEGRLQYRTWEAQDGSKRSAVDVVADNVQFL
                     GRPGDSEDGASSGGGGGQARRGGGRGGRREEVEIDETDFEDIPFRYFSIKPLVARDIL
                     "
     gene            complement(3901..4335)
                     /locus_tag="VN178_09225"
     CDS             complement(3901..4335)
                     /locus_tag="VN178_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS81192.1"
                     /translation="MGDKGLITDVIAPPTDAEGGLFSEINDNSVAILLTYGTARILLA
                     GDAEANEEEYMANGPYTGPLTILKVTHHGSNTSSTPLFLSRFPPKVAVIQCGADNPYG
                     HPTPETLDRLQRVGAKVFRNDEDGDVIATIKDQELKVATTKR"
     gene            complement(4302..>4643)
                     /locus_tag="VN178_09230"
     CDS             complement(4302..>4643)
                     /locus_tag="VN178_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81193.1"
                     /translation="GYLELAAEGILGRDELRAKLAVLEETRETAERELHALEGRSELL
                     KNLEREKDCIIERYAGLVPEALEELSPKERHRVYRMLRLSVVAHSDATLEVHGALSRA
                     GLGTKDLLRTS"
BASE COUNT          793 a         1543 c         1368 g          939 t
ORIGIN      
        1 aaggcgatca ggtcggggta cccctcctcg ccgcccacga cgggggtgtg gcgaagatcg
       61 gtccccatga ccgccgaaca gaacgcgagc ccgtgttcga tcgcacgctc gaattcacca
      121 atcggcacgt ctttgcccct cgccacgccg tgcaggtcgg ggtagagcac cctcacgcac
      181 gttacggact cgtcggacag ctcgggcggc ctggccacga gttcttcttt ctggaccacg
      241 actactcctt cccgccggcg gcgccggctg agttctcaga tcggtgtgtt gcggcctccg
      301 ccaagaatgc atcgaagagg cggaaggcgg cctccctggc cgcctcctcc cgctctggcg
      361 ggatggcaag gagatctctt ccgtctccta taccgagttt cgccagattc tcggtatccg
      421 cgccagccca atctgcgacg atctcgacgg tgctctccgg gtggaactgg accccgaggt
      481 gaggcccgag ccggaacgcc tgcacggcgt cggccgtgcg cgccacctcc acggcgcccg
      541 gtggcgtcgt gaaacgttcg tagtgccact ccagccaggg acctgagggg atcgcgcccg
      601 gatcgtcggt ctcgatctct cgccagccga gctccggcac cggcgcccgc tctacacgcc
      661 cgccgagcac ggcggagagc acctcgccac cgaagcacag cccgagcacg gggacatctt
      721 tctctaccgc ggccccgacc aactccagct ccgccaagac cccaggctcg tgcgtgtcgt
      781 tgggaccgta cggggaaccc agcgaagcca cgaacgaata gtcggatggg tcggggatcg
      841 acccgtcctt gtaggagtaa ttcacctcgt atgaaatgcc gcggtcctcc agccactcga
      901 ccagcaggcc cggcggcgtc atctcctcgt gctggcggat cagaaccggt ttcacccgcc
      961 ggcctccgag accatcttct cggcctcctc ctctgagacg gcgagggcga cgtgcagggt
     1021 gttggccgaa gtctcgggct gctcgcctgg cccggtgagg tccacgtccc actcctcgac
     1081 ggtattcgtc aggcgcccga tgccgtcgat ctccacttcg accacgtcgc cgggctccat
     1141 cggcctagag ttcgcgggtg tcccggtaag gaccacatca ccgggctcca gcgtgatcag
     1201 gcggcacaga tcggccagct ggtaggcgac ggggaagatc aaatcgtcgg atcctccctc
     1261 ctgcaccacc tccccgttga tataggtccg aagccggaag tcctccgggt cgaactcgtc
     1321 ggccgagacc acctcgggac cgagcggcaa gaacccgtcc tgccccttga cgcgcaacat
     1381 ggaaccccgg tccgcatgcc tgaaatcgtg caaccccacg tcgttggcac acgtgtagcc
     1441 gccgacgtaa gaaagggcat cttcttccgc cacccccttc atccggtgcc cgacgatgac
     1501 agccaactct ccctcgtagt tgaggaactc cgtcccccgc gggcgcctga tcacaccgcg
     1561 gtgcccgtta agggtcgtcg tcggcttgag gaagtacgac ggctgcggcg gagtgcgcgc
     1621 agcatactcg tctacgcggc tgcggtaggt caggtggacc gccaggatct tggtcggttc
     1681 cgccggcgcc agatactcgg cctccgcctc agggacccgt cccccccctt ccaggacaac
     1741 ctctcccccc tcgagcctgc cccacgcaga cccgccttcg tgcaggatcc ttacccttct
     1801 acccattcca gctcctttct tattggatac attatccaga aatggggtgt gtgtcaaggc
     1861 tctgcaagct cattgcataa tgtatccata aaggctatga tcgggtgatg gaatcggtcc
     1921 cccgttcgac cgcagcaagc gatcccatcc gggtcgactc cgtagcggac cttgcctacg
     1981 agcggatacg cgggtacgtg cttgggggtg aggttccgcc gggggcccgg ctcgggcagg
     2041 tggagctggc ggagaggttt gggatctcac gcacccccgt gcgcgaagcc ttgcgaaggc
     2101 tcgcgggcga gggtctggtg gacttccact ccaaccgtgg attccgggtc gccgatctcg
     2161 gcctggacgc ggtgctgcgc cgcatggagg tgcggtccat cgtggagccg gggatcgcac
     2221 gtctcgcggc ccagagaaga acggaagaag accttcagcg actcgaggag gccatcgcga
     2281 gcgaggaggg cgcgcgcgac gggatctccg cccacgacgc cagccgcgac ttccacatcg
     2341 cgctcgcgct cgcctcgggc aatgaggagc taatgcgggt actcaattcg ctctggctgg
     2401 tcgaagtcgg ccgtcggctc ttatccaggc gcaccgcaga tcccaactgg ctgcacgacg
     2461 acgtatcgga acaccgtgag atcctcaccg ccgtctcaga aggccgcgcc gccgacgccg
     2521 aacgcctcat ggccgagcac atcgctcgcg ccgtacacca ctgggagccg ctcgggagcg
     2581 gcggtacgag agacgggtag cctcagccat caaccaaaag agaggagctg aaagccgaaa
     2641 gccggccgct cctgcagggc ccgactcttc tgcaaaccac gctgatctgt aatcgatgac
     2701 gctcacgaat ttcttcctgt tgcacgtacc gcacaacggc tgtatgttgc cgatatcgtc
     2761 cgagcctccc ttggtcagag gtgcgacgtg gtcagcctcc agtaaggatt cggtagtgcc
     2821 gcaggcgaga cacttgttgc cgtaactctc gcagagcgcc ttgaactctg cggcggtaaa
     2881 actgcctgga ctggcgtgcc tcgcggccct cctcttgcga ctattgttgg ctttggcttg
     2941 cctgactttc tcaggatttt cctcggccca cttcttgctg cgagcaatta ccttttcgcg
     3001 ctcacgttcg tagtgcttac gcctggaagc gtttacgcta tcttcgttct ccgctgccca
     3061 tcatttcttg tactcagcga tctgttcctt gtgttcttcc tcgtactttc ggcgcacctc
     3121 gaccctatgg ggtaggttca ctcttgcttt ctcgtactca gcgtactgct ccaggtgctc
     3181 acgccggtac ttctgcgtgc gtgccctgat gcagaccttg cacttattca ggtggccgtc
     3241 cgtcatctga gggtgtttgt agaactccga aaggtttttc tcgactctac agcccctgca
     3301 gacttttgac ctcacctcac aagatatctc gggctacgag cggcttgata ctaaagtatc
     3361 gaaatggaat atcttcaaag tcagtttcat cgatttccac ctcttcgcgc cggcctccgc
     3421 gcccccctcc acggcgggcc tgtccgcccc cgccgccgga agaagcgccg tcctccgagt
     3481 cgccgggtcg gccgaggaat tgcacgttgt cggcgaccac gtcgacggcg ctccgctttg
     3541 agccgtcctg ggcctcccag gtacgatact gcaggcgtcc ttcgagtagg atggggtcgc
     3601 ccttcttctt gtagttggcg acggtctcgc cgagctcgcg ccaggcggtg atgttgaaga
     3661 agtccacctc ttcgctgcgc gacctcaccc tgttgacggc cagtccgaac gagcagactg
     3721 gtatgccgtc gttggtgaaa cgcagttctg gatctctggt caagttaccg gccagtacga
     3781 cacgattgaa gctgaccatg tctctcctcc ttctgtatgc cttacaaggg taacaataca
     3841 gcaacattct cggttctcaa gcgccatggc tttagccgga cgcaggtgga gtcgcgcaca
     3901 tcagcgtttc gttgtggcca ccttcagttc ctggtctttt atggtggcga tcacatcccc
     3961 gtcctcgtcg ttgcggaaga ccttcgcgcc taccctctgc aacctgtcga gggtctctgg
     4021 cgtgggatgc ccgtaggggt tgtccgcgcc gcactggatg acggccactt tgggcggaaa
     4081 gcggctgaga aacagcgggg tcgatgaggt gttggagccg tggtgggtca ccttcaaaat
     4141 cgtcagggga ccggtgtaag gaccgttcgc catgtactcc tcctcgttcg cctcggcgtc
     4201 gcctgcgagc aggatgcggg ccgtgccgta ggtcaagagg atggcaacgg agttgtcgtt
     4261 gatctcggag aacagaccgc cctcggcgtc cgtaggcgga gctatgacgt ccgtaataag
     4321 tcctttgtcc ccaatccggc tcgcgacaag gcgccgtgca cctcaagcgt tgcatcggag
     4381 tgcgccacca cacttaacct cagcatccta tagacgcggt gacgttcttt gggggagagt
     4441 tcttcgaggg cctctggtac caagccagcg tacctctcga tgatgcaatc cttctcacgc
     4501 tccaggttct taaggagttc gctccggccc tcaagcgcgt ggagttcgcg ctctgcggtc
     4561 tcgcgagtct cctcgaggac agcgagcttc gccctcaact cgtcgcgacc caagatgccc
     4621 tcagccgcaa gttctaggta gcc
//
LOCUS       DATMVF010000188        14337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_168116, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000188 DATMVF010000000
VERSION     DATMVF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168116"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VN178_09235"
     CDS             <1..804
                     /locus_tag="VN178_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWS81194.1"
                     /translation="LNLLGRVREAGRRNGEIVTGLAVKEYSEKYLSSEERAEGHPGEV
                     LIIVPAYDEEGGIQEVLRRIPDRVLGHEVKTVVVDDGSADRTADIARAEGVPVVSHVV
                     NRGQGDALRTGFAIAQAEKSEIVINLDADGQYKPEELDRLVKPIIEGRADFVLGSRFM
                     GYYEEAGSVRHIGVVFFSRMISLLTGVKVSDCTNGYRAIRVSELHKLNLREDRFNANE
                     IILEGLKNKLRFEQVPVSMMTRAAGETKKPPKLAYPLGVFRVIISTWLR"
     gene            941..2194
                     /locus_tag="VN178_09240"
     CDS             941..2194
                     /locus_tag="VN178_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWS81195.1"
                     /translation="MFKGQQGAGAGSVHIAASFGIAGVLTFAFQSLCTHVLGKQGFAP
                     LALLWSATFLTVQVLWIGATQTLGRYVAEREARGSDWGPVVSSVRHWQIGLVVAFVLC
                     ALLAGPLLTDELFEDPWLTVAFIVAVVLYAPEYFRRGIFNGHRQSFRLGAQIVVESSG
                     RLLIAAVLLVVGWGVFGPVVAIVLAPLIGVLAVRPARAAAPEREGEPFSAWQAFRFAA
                     PVLACMAFAQALMNGGPILARVLDGSDAQVSVFSAALIFTRIPQYVLSPAIGALLPRA
                     SRVLSTEGRGAFDRFVAKALGVVGLVGVLMVAGTWTFGEWGLRIFAGAEFDAGLAVLV
                     ALATMAAFYLLSDALNQGLFALGQGRLAALGWFVGLLACGVCLTALGFGVVERVAYSL
                     ALGTFVAAVAQGMFYVGMRPGAAGR"
     gene            complement(2191..3177)
                     /locus_tag="VN178_09245"
     CDS             complement(2191..3177)
                     /locus_tag="VN178_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWS81196.1"
                     /translation="MNWLVTGGCGFIGTALIRSLMREGDHAVRVVDNLSVGTQEDLAA
                     AADFVEVSSEELGPIAAGERVELVAGDITDEDLALRAARGADAIVHFAANTGVMPSVE
                     DPRGDCMSNVFGTLNYLEAARLGGVGRFVFASSGGTVIGEAEPPIHEEMVPHPVAPYG
                     ASKLAGEGYCSAYFQTFGVETVALRFGNVYGPLSGHKNSVVARFIKRAVEGEVLEIYG
                     DGTQTRDFIFVDDLVGAVRLSATVEGVGGEVFQIATSAETTVQELTDRLLPALAAEGV
                     GNIEVRKTAARRGEVRRNYADTSKARRLLGWKADVGLDEGLRRTVRWFLGRG"
     gene            3313..3495
                     /locus_tag="VN178_09250"
     CDS             3313..3495
                     /locus_tag="VN178_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81197.1"
                     /translation="MKAKNVLRRGTLFATISGGWCDVVEKASKMNIKMLLAPKNYGGR
                     MNADALSCRRLSGGEG"
     gene            3492..4982
                     /locus_tag="VN178_09255"
     CDS             3492..4982
                     /locus_tag="VN178_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWS81198.1"
                     /translation="MKERTLTRRDFLKVAGAGAAGVTMLGAAGCGSLANSFTQVPDEY
                     LPSGGSRMNVIVVIIDSLRRDHLGVYGNEWIKTPNLDALARESLRFDRSYPESLPTIL
                     ARRAIHTGIRTFPFRDWQKWYDEDVSLWGWQPIPRGQVTLAEILLGEGFYNLMVTDTL
                     HQFRPFYDMHRGFHAFDFIRGQERDLFRPPSPASEKKMENTLIGGPSAAHSEEIMHQY
                     YANTVGRQREEDWFAPQVFTKAAEYLEMAKESQPFFMFVDNYDPHEPWDPPQKYVDLY
                     SDAYDGPEPVTSSSGPSDWLTQRQLERMHARYSAEVTMTDHWLGHFLDKAQDLGILDN
                     TLLIVQSDHGHAFGEHGYAGKVAGALYPELTDTIFFMRHPGGKGADQTSDHYASTHDV
                     APTILGAMGLEPHPQMGGQDLSVVLDGKEPDPRPHFTAGYHDHVWARDEKYAMFSLYN
                     GEDAHLFDLESDPKMDKDIASSNPDVAKKMFDEYVVKDAGGPLPHY"
     gene            5028..5645
                     /locus_tag="VN178_09260"
     CDS             5028..5645
                     /locus_tag="VN178_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81199.1"
                     /translation="MRRLLTFLLEWSGILCVPGGLLWALSPTGISISNLAFHTPNVFW
                     KMFWSAPLLILLGLIGLQLRQSERSGLWEKLGFFVAALGLVLIIAGDIGLYWLGIDDR
                     FIVTAPAYNAFRIGLLVLAVGSILFAVTAPRDGALPTWGLLPFVLGSMCGLISFSRDF
                     GSFGAVLWALFGIGWAWLGLSLVVGGFSSFLKTRRATRDGIAIKH"
     gene            5725..6123
                     /locus_tag="VN178_09265"
     CDS             5725..6123
                     /locus_tag="VN178_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HWS81200.1"
                     /translation="MDIEAVLKRGEGVGKSLGIEFVELTPQRVVATMPVDERHHQPFG
                     YLHGGVSVVLAESVASTGAFLNCPPGKVAFGSEISASHVRPKRSGTLTAVGVPAHVGR
                     RSQVWEVRITDEEDKTVCVSRCTTTIVDAE"
     gene            complement(6136..7077)
                     /locus_tag="VN178_09270"
     CDS             complement(6136..7077)
                     /locus_tag="VN178_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWS81201.1"
                     /translation="MKLRTALLAMGGISGGLALYNRRLERGGGQLLNRLGGEVRSYRW
                     GGGDLAYSVAGEGEPLLLVHGVYAGASSFEFRKNFQELSRSFRVYALDLLGCGMSERP
                     SRRYGPEDVAVQVEDFVREEIGGATHLVASSLTAALAVPAVVRSPRLFKKLVLICPTG
                     YGTLDRQSGRLGDVIYGLFLAPVFGNTLYHAIVSRWGIRYYLGRMAYHDADLVTEELV
                     EDYHKVSHQIGARYFPAAFVSGKLNLGVAGLWPRVPHRTLICWGLQARTVPVREGQRF
                     VHNNPRSEPRIFKDAALLPHDERAGTFNEEVRTFLLE"
     gene            7212..7685
                     /locus_tag="VN178_09275"
     CDS             7212..7685
                     /locus_tag="VN178_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWS81202.1"
                     /translation="MDRERLVDETLMLLPTLMRLVERPSPVEMGELSRRGVATDVQVS
                     PGHIQVLIALTRGPRSVGQLAEELGVSPPAATQLVDRLVEHGMVDRHNDPEDRRVVLV
                     DYVAGMHEVAGRIVGDRRRPLHDAMSKMTDGEALAFVKGLRLLAQSFGAPAREGI"
     gene            7685..10966
                     /locus_tag="VN178_09280"
     CDS             7685..10966
                     /locus_tag="VN178_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWS81203.1"
                     /translation="MQRIVRWCLKNKSVVILATILLIGSGVYATTRLNQELLPDISFP
                     IITISTPVTGAGPDLVDEQVTQPVEDAINGVQGIEAVQSTSSQGFSVVVVEFDLDADT
                     EEAEADLQAALDGIQLPSQAGDPEVKSQSASQFPILSLSLAAGDQDLADLTSYAEDEV
                     VPSIEEVEGVASVDLIGGAEKRIQVDLDPEKMKEKGLSTDAVVGALSGAEVDAPVGSV
                     TVNGRETPVNTSSELGGISALEDLPVGVAGGDTGGAPPAAATDVPAAVPSTAAPPGGA
                     PAGAPTGAPTGTSAAPGGVPAGPSALPVGSAAGTAGSPAAAAEPVLLGDVAEVREVGS
                     GLSGISRTNGEPSLGLNVIKETDANTVEVAEEVERVLDDVRDRIGPDQVVVVANSATD
                     VEESVNGLVEEALIGAVLAILVIFAFLRSMRATLVTAVSLPTSVLAALLFSWADNLTL
                     NIITLAGLTIAVGRVVDDAIVVLENSYRYVQGGHEPEESALKGTTEVASAITSSTLTT
                     IAVFLPLALVGGIISKFFVPLSLTVALALVASLIVAVTIIPVLVSIFLRRRMGDRTSQ
                     GADEDASEDPENGSRRRSGGESGGLLLGLYTPMLCWSLRHRLAVLLLALLTFAGGLGL
                     VRFLPVTFFPPSEERLLIADVELPAGTALGRTSERLRPFEAFLQQDAGIKSYQVSIGG
                     EDTQDPESPVRPGNKAQAFINVKESVDVRRTLDRVDGEGDRLYGENFQVQILSNGPPQ
                     GGLEAVLTGGSKGELAKAADIVSDEFRKLEDVNNVESDLSGGNPEVEIKVDPEKAAKA
                     GLSPAVVSGSLGSLLGESTVTTLGDTPVVVGVPEDSVDSLDEVRGLPVGSDTTVGDVA
                     EVGEVESPAAISRTDGERAVTVTGGITSTDTQTVSTEAQRAIADLDLPGNVTAQVGGE
                     NEDIDESFRNLFLSIIVALVLVFLLLVVFFGSLLVPMVILLAVPLTTVGAFGALYLTN
                     TAISVPSLLGLLLLIGIVVSNAILLVDFAVKAQDRYETSDEAILAAGQARLRPILMTA
                     FATIFALMPLALGLSGGGNGLISSSLAITVVGGLATSTFLTLLVVPVGYSLLKSGPRR
                     KGKKG"
     gene            11126..11332
                     /locus_tag="VN178_09285"
     CDS             11126..11332
                     /locus_tag="VN178_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81204.1"
                     /translation="MREVLKDRFESIPVQVPRSEIEALGVEIGLEDPTEAARIFDRLK
                     GVSWRGDYVRTEEGWVAAWVMEIR"
     gene            complement(11606..13285)
                     /locus_tag="VN178_09290"
     CDS             complement(11606..13285)
                     /locus_tag="VN178_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HWS81205.1"
                     /translation="MKTNFDAYLRYGELTRAMHALAEEHPEFCKVESIGKSNEGREIW
                     LAELTNLETGPAETKPAFWVDGNTHAGEVTGSMAALYLIEHLLEKYGAEDLPTRLLDE
                     QAFYVLPRLSPDGAERYLTTAHTLRATPRPWPEPEEEPGLHPEDVDGDGDILQMRVED
                     ENGEWRVSDKDPRLMVPRTPDEADSEATYYRLYREGTFKDYDGFARKIARPLYGLDMN
                     RQYPYRWQAEDEQLGAGPYPLSEPESRAQVDFLLDHKNVFGAHTYHTFCGAILRPYSD
                     RPDDEMPEHDLAVFEAIGERGTRITGYPNISVYHDFRYDPKKSITGAFDDWAYDSYGV
                     FAFTVEFWSMAQAAGVEVEDFIEFFRDPPEEASLKMLAWNDRELGGDGFVPWRPFDHP
                     QLGRVELGGWKTKFTTQNPPPRFLRAECEKLARFALSHAATAPRLQTSLKTEQLSQGL
                     RRVELIVENAGYLPTNVTRMAADKELAKPVEAEIELPTDASLVSGEREVELGHLAGRS
                     ALAGNRWKSPAFFEGLPSDYAGRAVWVVRGEGPVRVEVRGGRAGTARLVTG"
     gene            13525..>14337
                     /locus_tag="VN178_09295"
     CDS             13525..>14337
                     /locus_tag="VN178_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWS81206.1"
                     /translation="MESTLGSGGMAVVYRAEDDILGRAVALKTLHHHFAEMPSFRRRF
                     RQEARAMASLDHENIVKVYDISQDGEVPFIVVECVSGRDLGDLLGGSRRHSGRLNEQS
                     VRRMAIQLLHALSYAHGRGIIHRDIKPSNILLTSEGTVKVADFGIARIVEEDDAGDAG
                     EIVGSARYMSPEQLRGEAATPRSDVYSVGVLLYHCLTGRPPFSGDVKSLARQQIHKEP
                     TPPRRLNKRITPEMEAVIMKALSKKPRDRYFSAEAMLDDIERESPPRPAGTTA"
BASE COUNT         2508 a         4596 c         4657 g         2576 t
ORIGIN      
        1 ctgaacctcc ttggccgcgt gcgagaggcc ggacgccgca acggcgagat cgtcaccggt
       61 ctcgccgtaa aggagtactc ggagaagtac ctctcctccg aagagagggc cgagggacac
      121 cccggcgagg tcctgataat cgtgcccgcc tacgacgagg agggcggcat ccaggaagtc
      181 ttgcgccgca tcccggaccg ggttctgggt cacgaggtca agacggtcgt cgtcgatgac
      241 ggctcggccg acaggacggc cgacatcgcc agggccgagg gcgtgcccgt ggtgagccac
      301 gtggtcaacc gcgggcaggg cgacgccctc aggacgggct tcgccatagc gcaggccgag
      361 aagtccgaga tcgtcatcaa cctcgacgcc gacggccagt acaaaccgga ggagctggac
      421 cgcctcgtca agcccataat cgagggcagg gcggacttcg tgctcggctc ccgctttatg
      481 ggctactacg aagaggcggg gagcgtccgc cacatcgggg tggtgttctt ctcgcgcatg
      541 atctcgcttc tcaccggcgt caaggtctcc gactgcacca acggctaccg cgccataagg
      601 gtctcggagc tccacaagct gaacctgcgc gaagaccgct tcaacgccaa tgagatcatc
      661 ctcgaaggcc tcaagaacaa gctgcgcttc gagcaggtcc ccgtctcgat gatgacccgc
      721 gcggcgggcg agaccaagaa acccccgaag ctggcctacc ctctgggggt cttcagggtg
      781 attatctcaa cgtggttgag ataaaggcat caggtttcag gaccgctact agggtggccg
      841 tcttcgccaa caacgtctct ctgcccggag gatgccagga cgatcgctta acgggataca
      901 ctctcgttca tgataaaaga cctgaaacct gaggcctaac ttgttcaaag gacaacaggg
      961 agcgggcgcc gggagcgtcc acatcgcggc atccttcggc atcgccgggg tgctcacgtt
     1021 cgcgttccag agcctctgta cccacgtcct cggcaagcaa ggcttcgcgc cgctggccct
     1081 cttgtggtcg gccacgttcc tcaccgtgca ggtgctctgg atcggcgcca cccagaccct
     1141 ggggcgctac gtcgccgaga gggaggcccg gggcagtgat tggggtcccg tagtctcctc
     1201 ggtcaggcac tggcagatcg ggctggtagt ggcgttcgtg ctctgtgctt tgctggcggg
     1261 gccgctgctc accgatgaac ttttcgaaga cccgtggctc accgtcgcat ttatcgtcgc
     1321 cgtcgtcctc tacgcgcccg aatatttccg gcgcggcatc ttcaacgggc acaggcagtc
     1381 ttttcggctc ggcgcccaga tcgtggtcga gtcatccggc aggttgctca tcgccgccgt
     1441 gctgctcgtc gtgggatggg gtgtcttcgg accggtcgtc gccatcgtgc tcgccccgct
     1501 catcggcgta ctcgccgtca ggccggcccg ggcggcggcg ccggaacggg aaggagagcc
     1561 cttctcagcc tggcaggctt tccggtttgc cgcgccggtg ctggcttgta tggccttcgc
     1621 gcaggcactg atgaacggtg gtccgatcct cgccagggtc cttgatggca gcgacgcgca
     1681 ggtgagcgtg ttcagtgcgg ctttgatctt cacgcgcatc ccccagtacg tcttgagccc
     1741 ggccataggc gcgctcctgc cccgggcgag ccgcgtgctc tccaccgaag gcaggggggc
     1801 cttcgatcgc tttgtggcca aagcgctagg ggtcgtcggc ctcgtgggtg tgctcatggt
     1861 cgccggcacc tggacgttcg gcgagtgggg gctcaggatc ttcgccggcg cggagttcga
     1921 cgcaggcctg gccgtgctcg tcgccctggc gacgatggcc gccttctacc tcctctccga
     1981 cgcgctcaac cagggactct tcgcactagg gcagggtcgt ctcgcggcgc tcgggtggtt
     2041 cgtcgggctg ctcgcctgcg gcgtctgcct gacggcgctg ggcttcggtg tcgtcgagcg
     2101 ggtcgcgtac tccctggccc tcggcacctt cgtcgccgcc gtggcccagg gtatgttcta
     2161 cgtggggatg cgacccggag ccgcaggccg ctagcccctc cccaagaacc atctcacggt
     2221 gcgcctgagg ccctcgtcga ggccgacatc ggccttccag ccgagcaatc gccgcgcctt
     2281 ggaagtgtcg gcgtagttgc gacgcacctc cccgcggcgg gcggccgttt tgcggacctc
     2341 gatgttccct acaccttcgg cggcgagggc gggaagcagc ctgtccgtca gctcttgcac
     2401 cgtcgtctcg gcgctggtgg cgatctggaa gacctcgccg cccacgccct cgacggtggc
     2461 cgagagcctc acggccccga cgagatcgtc cacgaagatg aagtcgcggg tctgggtgcc
     2521 gtcaccgtag atctccagca cttccccctc cacggccctc ttgataaagc gcgcgacgac
     2581 gctgttcttg tgtcccgaga gtggtccgta gacgttcccg aagcgtagcg cgacggtctc
     2641 tacgccgaag gtctggaagt acgccgaaca gtagccttct ccggcgagct tgctcgcccc
     2701 gtagggcgcg acgggatggg ggaccatctc ctcgtgtatc ggcggctcgg cctccccgat
     2761 aaccgtcccc ccgcttgagg cgaagacgaa gcgccccaca ccgcccagac gggcggcctc
     2821 gaggtagttg agcgtgccga agacgttgct catgcagtcg cccctggggt cctcgaccga
     2881 gggcatcacc cccgtgttcg ccgcgaaatg gacgatggcg tcggcgcccc ttgcggcgcg
     2941 tagcgcgagg tcttcgtccg ttatgtcgcc cgcgaccagc tcgacccgct cgccggcggc
     3001 tatggggccg agctcttcgg acgatacttc gacgaaatcc gcagcggcgg ccaggtcctc
     3061 ctgtgtccct acagagaggt tgtcgacgac cctgacggcg tggtcgcctt cccgcatgag
     3121 agagcggatc agggccgtcc cgataaagcc acagccgccc gtgaccagcc agttcacgag
     3181 gaccactcca gagagagatc accttccgag catcttgtat ctgcgtgcat acccgtcctt
     3241 gccgagagta gtgtgtcgcg cgtaccttag accaacctcc cctccggtgc gagtctcggg
     3301 acgcatgagg gtgtgaaggc gaagaacgtc ctgcggaggg ggactctatt cgctacaatc
     3361 tccggcggct ggtgcgatgt ggtagagaag gcatcgaaga tgaacatcaa aatgctcctc
     3421 gctccgaaga actacggcgg tcgaatgaac gccgacgccc tgtcctgccg gcgcttatcc
     3481 ggtggcgagg gttgaaggag cgcaccctca cgcggaggga ctttctcaaa gtggccgggg
     3541 caggggccgc cggggttacc atgctcggcg cggccgggtg cggcagcctg gcaaacagct
     3601 ttacacaagt gcccgacgag taccttccga gcgggggatc gagaatgaac gtgatagtgg
     3661 tgatcataga cagcctgaga agggaccacc ttggcgtcta cggcaacgaa tggataaaga
     3721 cccccaacct cgacgccctc gccagagaga gcctgcgctt cgaccgctcc tacccggagt
     3781 ctctgccaac tatattggcg cggcgcgcta tccacacggg gataaggacg ttcccgttca
     3841 gggactggca gaagtggtac gacgaggacg tcagcctctg gggctggcag cctatcccca
     3901 gaggacaggt taccctcgcc gagatcctcc tgggagaagg tttctacaac ctgatggtca
     3961 ccgacacgct gcaccagttc aggcccttct acgacatgca ccgcggcttc cacgccttcg
     4021 acttcatcag gggccaggag agggacctct tcaggcctcc gtcgccggcc tccgagaaga
     4081 agatggagaa caccctgatt ggtggcccga gcgccgcgca ctccgaggag atcatgcacc
     4141 agtactacgc gaacaccgtg ggccgccaga gggaggagga ctggttcgcc ccgcaggtgt
     4201 tcaccaaggc ggccgagtac ctggagatgg cgaaggagag ccagcctttc ttcatgttcg
     4261 tcgacaacta cgacccgcac gagccgtggg acccgccgca gaagtacgtc gacctctaca
     4321 gcgacgccta cgacggcccc gagcccgtga cctccagcag cggtccctct gactggctca
     4381 cacagcgcca gctcgagcgg atgcacgcgc gctactccgc cgaggttacg atgaccgacc
     4441 actggctggg ccacttcctc gacaaggccc aagaccttgg aatcctggac aacaccttgc
     4501 tcatagtgca atcggatcac ggacacgctt tcggggagca cggttacgcc ggcaaggtcg
     4561 ccggcgcgct ctaccccgag ctcaccgaca ccatcttctt tatgaggcac cctggcggca
     4621 agggcgcgga ccagaccagc gaccactacg cctctaccca cgatgtggcc ccaacgatac
     4681 tcggcgcgat gggcctcgag ccgcacccgc agatgggggg ccaggacctc tctgtagtgc
     4741 ttgacggaaa ggaaccggac cccaggcccc actttacggc cggctaccac gaccacgtct
     4801 gggcccgcga cgagaagtac gccatgttca gcctctacaa cggcgaagac gcccacctct
     4861 tcgacctgga aagcgacccg aagatggaca aggacatagc ctcgtcgaac cccgacgtgg
     4921 cgaagaagat gttcgacgag tacgtggtca aggacgccgg cggacccttg ccccactatt
     4981 gatcgggagc gcctcgatgt tatcgtaccg gagaggtaag agaactcttg cgtaggctgt
     5041 tgacattcct gctggagtgg agcggcatac tgtgcgtccc cggtggcctg ctgtgggcgc
     5101 tctcgcctac cggcatctcc atctccaatc tcgctttcca cacccctaac gtcttctgga
     5161 agatgttctg gtcggcacca ctgctcatcc tcctcggtct gatcggtctg cagctgcggc
     5221 agtcagagcg ttccggcctg tgggagaaac tggggttctt cgtggccgct cttgggctcg
     5281 tcttgatcat cgccggcgac atcggccttt attggctcgg gatcgacgac cgctttatcg
     5341 tgacggcgcc ggcgtacaac gccttccgga tcggactgct cgtcttggcc gtgggatcca
     5401 tcctcttcgc cgtcacagcc ccccgcgacg gcgctctgcc tacctggggc ttgctaccct
     5461 tcgtcctcgg gtcgatgtgc ggtctgatct ccttttcgcg ggactttggc tccttcggcg
     5521 ccgtgttgtg ggccctgttc ggtatcgggt gggcgtggtt gggactctcg ctcgtggtcg
     5581 gaggcttctc ctctttcctg aagacgagac gtgccacccg cgatggtatc gcaattaaac
     5641 actgacacga agagtctaga gaccttcctt gcccgaccgg ctagtatact ggcggctgtt
     5701 cgagagatct ggagggcggg agccgtggac atagaggccg ttctcaagcg tggtgagggt
     5761 gtcggcaaga gcctcggcat cgagtttgtg gagctcacgc cgcagcgggt ggtagccacg
     5821 atgcccgtgg acgagaggca ccaccagccg ttcgggtacc ttcacggtgg ggtcagcgtg
     5881 gtgctggcgg agagcgtggc gagcacgggc gcattcctga actgtccgcc ggggaaggtc
     5941 gccttcgggt cggagatcag cgccagccac gtcagaccca agcggagtgg gacgctcacg
     6001 gcggtcgggg tgcccgcgca cgtggggcgc aggagccagg tgtgggaagt tcgcatcacg
     6061 gacgaagaag acaagacggt atgcgtctcg cgctgcacca ctacgatagt tgacgccgag
     6121 tgaatgggga agggatcatt cgagtaggaa cgtcctgacc tcttcgttga aggtcccggc
     6181 gcgctcgtcg tgcggcagga gggcggcatc cttgaagatg cgcggttcgg aacgggggtt
     6241 gttgtgcacg aaacgctgcc cctcgcgcac ggggaccgta cgcgcttgga ggccccagca
     6301 gatcagggtc ctgtgcggca cccgcggcca gaggccggcg accccgaggt tgagtttgcc
     6361 cgagacgaag gccgcaggga agtacctcgc cccgatctgg tgacttacct tgtggtaatc
     6421 ctcgaccagc tcttctgtca cgagatcggc gtcgtgatag gccatgcgcc cgaggtagta
     6481 gcggataccc caccgggaga cgatggcgtg gtagagcgta ttcccgaaga ccggcgcgag
     6541 aaagagaccg tagatcacat cccccagccg tcctgattga cggtccagag tcccgtaacc
     6601 cgtcggacag atcaaaacca gcttcttgaa gagccgcggg ctccgcacga cggccggcac
     6661 ggcgagcgcc gccgttagcg agctggccac gagatgggtc gcgcccccga tctcctcccg
     6721 cacgaagtcc tcgacctgca ccgcgacgtc ctcgggcccg tagcgccgcg aaggcctctc
     6781 cgacatgccg caccccagga gatccagggc gtataccctg aagctcctcg acaactcctg
     6841 gaaattcttg cggaactcga aagaagacgc cccggcgtat acgccgtgca ccaacagcag
     6901 cggctcaccc tcccccgcca ccgagtacgc gaggtcccca ccgccccacc ggtaggagcg
     6961 cacctcaccg ccgagccggt tcagaagctg cccaccaccc ctctccaacc gccggttgta
     7021 gagcgccaac cccccgctaa tgccccccat cgccaggagc gccgtcctca gcttcatccc
     7081 gcctcctaaa gagatgctcg atgccaggat cgtaccgaaa gcctcggcca gattcctcgt
     7141 tgacaaaccc caagacgggg atagcatctg agtaaattac ataagcggct taaatttgta
     7201 ggtgaattca catggacagg gagcggctcg tcgacgagac tttgatgttg ctaccgactt
     7261 tgatgcgcct ggtcgagcgg ccatctcctg tggagatggg tgagctctcg cgtcgcgggg
     7321 tggcgacgga cgttcaggtc tcgccggggc acatccaggt cttgatagcc ttgaccagag
     7381 ggccgaggtc cgtcgggcag ctcgccgaag agttaggggt ctcgccaccg gcggcgacgc
     7441 agctcgtcga caggctggtc gagcatggga tggtagaccg ccacaacgat cccgaagacc
     7501 ggcgggtcgt cctcgtcgac tacgtggcgg gcatgcacga ggtggccggg cgcatcgtgg
     7561 gggatcgtcg gcgtcccctc cacgacgcca tgagcaagat gacggacggg gaggctctgg
     7621 ccttcgtcaa gggcttgagg ctgttggccc agagcttcgg cgcccctgcc agggagggga
     7681 tctgatgcag aggatcgtcc gttggtgcct gaagaacaag tccgtcgtga tattggcgac
     7741 gatcttgctc atcgggagcg gggtctatgc gacgacgcga ctcaaccagg agctgttgcc
     7801 cgacatctcc ttcccgatca taaccatctc gacccccgtg accggcgcag gaccagatct
     7861 ggtcgacgag caggtaacgc agcccgtcga agacgccata aacggggtgc aggggataga
     7921 ggcggtccag tccacttcct cacaggggtt ctccgtcgtg gtcgtcgagt tcgacctcga
     7981 cgccgacacc gaggaggccg aggcagacct ccaggctgcc ctcgacggca tacagcttcc
     8041 ttcgcaggca ggagatcccg aggtcaagag ccagtcggct tcgcagttcc ccatcctgtc
     8101 gctctcgctg gccgcgggag accaggatct cgccgacctc acaagctacg ccgaggacga
     8161 ggtcgtcccc tccatcgagg aggtcgaagg cgtggcgagc gtagacttga tcgggggcgc
     8221 cgagaaacgc attcaggtgg atctcgaccc cgagaaaatg aaggagaagg gcctctccac
     8281 cgacgccgtg gtcggtgcgc tgagcggggc tgaggtggat gctcccgtgg gtagcgtcac
     8341 cgtaaacggg cgcgagactc ccgtaaatac cagcagcgaa ctcggaggga taagcgccct
     8401 cgaagacctt cctgtagggg tggcaggcgg cgacacgggc ggggcccctc ccgctgctgc
     8461 cacagatgtt cctgccgccg ttccgagtac tgccgctccg ccgggtggcg ctccggccgg
     8521 cgctccgact ggggccccga ccggcacgtc cgccgctccg ggtggagtcc cggccggacc
     8581 ttctgctctt cccgtgggta gcgccgcggg cacggcggga tctcctgctg cggcggcgga
     8641 gccggtgttg ctgggggatg tggccgaggt gcgcgaggtg ggctccggcc tgtcggggat
     8701 ctcccgtacc aacggggagc cgagcctggg cctcaacgtg atcaaggaga ccgacgccaa
     8761 caccgtcgag gtcgccgaag aggtcgagcg agtactcgat gacgtgcgcg acaggatcgg
     8821 gcccgatcag gtcgtggtcg tggccaactc ggcgaccgac gtcgaggagt cggtgaacgg
     8881 gctcgtcgag gaggcgctca tcggggccgt gctcgcgata ctggtgatct tcgctttcct
     8941 gcgttccatg cgtgcaaccc ttgtcaccgc ggtctctctg cccacctctg tgctcgccgc
     9001 cctcctgttc tcctgggcgg ataacctgac gctgaacatc atcaccctcg ctggcctgac
     9061 tatcgccgtg ggccgggtgg tcgacgacgc gatcgtggtg ctagagaact cctaccgcta
     9121 cgtgcagggc ggccacgaac cggaggagtc tgccctcaag gggaccacag aggtggccag
     9181 cgccatcacc tcatcgacgc tgaccacgat cgccgtcttt ctacctctgg ccctcgtcgg
     9241 cggaataatc tccaagttct tcgtcccgct ctcgctgacg gtcgccctcg ccctggtagc
     9301 ctcgttgatc gtggccgtga ccattatccc cgtgctcgtc agcatcttcc tcaggcgtcg
     9361 aatgggagac cgcacgtcac agggggcgga cgaagatgcg agcgaggacc ccgagaacgg
     9421 gagcaggcgg cgttccggtg gcgagtcggg cgggctgctc ttggggctct acaccccgat
     9481 gttgtgctgg agtctgcggc acaggctggc cgtgttgctg ctcgcgctcc ttacattcgc
     9541 ggggggactc gggctcgtgc ggtttctgcc cgtcacgttc ttcccgccga gcgaggagcg
     9601 tctgctcatc gctgacgtgg agctgcccgc gggcacggcc ctcgggagga cgagcgagag
     9661 gctgcgcccg ttcgaggctt tcctgcaaca ggacgcgggc ataaagagtt accaggtatc
     9721 catcggcggg gaggacaccc aggaccccga gtcccccgtc aggccaggca acaaagccca
     9781 ggcatttatc aatgtaaagg agagcgtaga cgtcaggcgg acgttggacc gggtggacgg
     9841 tgagggtgac aggctctacg gggagaactt ccaggtccag atcctctcga acggcccgcc
     9901 acagggcggg ctagaggccg tcctcaccgg tgggtccaaa ggagagctcg caaaggcggc
     9961 ggacatcgtc tccgatgagt tccgcaagct cgaagatgtg aacaacgtgg agagcgacct
    10021 ctccggcggc aaccccgagg tggagatcaa agtagacccg gagaaggcgg ccaaagccgg
    10081 gctctcgccc gcagtggtct ctggctcgct cggctccctt ctcggcgaga gcaccgtgac
    10141 gacgctcggg gatacgcccg tcgtggtcgg agtacccgag gactccgtgg attcgttgga
    10201 tgaggtacga gggttgcctg tggggagcga cactaccgtg ggagacgtcg ccgaggtggg
    10261 ggaggtagag tctccggcgg ccatcagccg aaccgacggg gagcgtgccg tcacggtcac
    10321 cggtgggatc acctccacag acacccaaac ggtctccacg gaagcccaga gggccatcgc
    10381 cgacctcgat ctgccgggga acgtcacggc ccaggtcggc ggcgagaacg aggacatcga
    10441 cgagagcttc aggaacctct tcctctccat aatcgtggcg ctagtgctag tcttcctgtt
    10501 gctcgtcgtc ttcttcggct cgctactcgt cccgatggtg atactgctag ccgtgcccct
    10561 tacgacggtc ggggccttcg gcgccctcta cctgacgaat acggccatct ctgtgccttc
    10621 gctactcggt ctcctgctgc tcatcgggat cgtggtctcc aacgccatcc tgctcgtcga
    10681 cttcgccgtc aaagcccagg accgttacga gacctccgat gaggctatcc tggcggccgg
    10741 tcaggcacgc ctcaggccca tcttgatgac ggccttcgcc accatcttcg ccttgatgcc
    10801 gctcgccctc gggctctccg gcggcggtaa cggcctgatc tcctccagcc tcgccataac
    10861 ggtcgtcggg ggcctcgcga cctcgacgtt cctgacgctg ctcgtcgtgc cggtcggcta
    10921 ttcgcttctc aagagcggcc ccagacggaa ggggaagaag ggatgagtta acgctcgtct
    10981 gcagatctgc cgaacataga ccttgcgtag tgttttgtac gacagattcg tcccctaagg
    11041 aagggcagac tatctgtatt cgaagaggag gtcggccatc agagagggca gataccggac
    11101 ccccaacagg cagcagcaga tcaggatgcg cgaggtactg aaggacaggt tcgagagcat
    11161 ccccgtccag gtcccgagga gcgagatcga agccctgggc gtcgagatcg ggctcgaaga
    11221 cccgacggaa gccgccagga tcttcgaccg gctcaaaggc gtctcctgga ggggcgacta
    11281 cgtgaggacc gaagaaggct gggtcgccgc ctgggtcatg gagatccgct gaattcttag
    11341 gaattcagcg gatcagcaat tcagcacgct caggctagcg ccttcgcttg tcagcccgcc
    11401 gcctccctcc ggtcggcggc tagtcagcat ttcagctaat cgtttcgtag ccccgccgag
    11461 agcgtaggac ccgactgccg cttgaagcgg ttcgtcgtac gcagcagggc gaggccagaa
    11521 cgggagaagc tgactcgctg acaagcgagg gcctgctaag gcccgaagcg tgctgacaag
    11581 gcgccgaagg cgccgtgctg aagtgctacc ctgtgacgag ccgcgctgtg cctgccctcc
    11641 ctccgcgcac ctcgacccga acgggtcctt cgccccgcac gacccagacg gcccggccgg
    11701 cgtagtccga aggaagccct tcgaagaagg ccgggctctt ccatcggttg ccggcgagcg
    11761 cggagcgccc ggcgaggtgt cccagctcga cctcccgctc tcccgatacc aggctcgcgt
    11821 ccgtcggcaa ctctatctcg gcttccacgg gtttggcgag ctctttgtcg gcggccatcc
    11881 tggttacgtt cgtcggcagg tatcccgcgt tctcgacgat gagctcgaca cgacggagcc
    11941 cctgggagag ctgctctgtc tttaggctgg tctgcaggcg cggggccgtg gccgcatggg
    12001 agagggcgaa acgggccagc ttctcgcact ccgcacggag gaatctgggc ggcggattct
    12061 gggtggtgaa cttggtcttc cagcccccca actccacgcg tccgagttgg gggtggtcga
    12121 agggcctcca ggggacgaag ccatcacctc cgagctccct gtcgttccag gcgagcatct
    12181 tcagcgacgc ttcttctggc gggtcgcgga agaactcgat aaagtcctcc acctccacgc
    12241 cggcggcctg cgccatggac cagaactcga cggtgaaagc gaaaacaccg taggaatcgt
    12301 aagcccaatc gtcgaaggcg ccggttatgg acttcttcgg atcgtagcgg aagtcgtggt
    12361 agacggagat gttcggatag ccggtgatcc gggtgccccg ctcgccgatc gcctcgaaga
    12421 cggcgaggtc gtgctcgggc atctcgtcgt cgggcctgtc ggagtagggc ctgaggatgg
    12481 cgccgcagaa cgtgtggtag gtgtgcgccc cgaagacgtt cttgtggtcg agcaagaaat
    12541 ccacctgcgc cctcgactcc ggctcggaga gcgggtatgg tcccgccccc aactgctcgt
    12601 cctcggcctg ccagcggtaa gggtactggc ggttcatgtc aaggccgtaa agggggcgtg
    12661 cgatcttccg tgcaaagccg tcgtagtcct tgaaggtccc ctccctgtag agcctgtagt
    12721 aggtggcctc ggagtcggcc tcgtcggggg tcctggggac catgagcctg gggtccttgt
    12781 cggagacgcg ccactcgccg ttctcgtcct cgacgcgcat ctggaggatg tcgccgtcgc
    12841 cgtcgacgtc ctcggggtgt aggccaggct cctcttcggg ctcgggccag ggtcgcgggg
    12901 tcgcccgcag ggtgtgggcc gtcgtcaagt agcgctcggc gccgtcgggg gagagccggg
    12961 ggagcacgta gaaggcctgc tcgtccagga ggcgggtcgg gaggtcttcg gcgccgtact
    13021 tttcgaggag gtgttcgatc aagtagagcg ccgccatcga gcccgtgacc tcgccggcgt
    13081 gggtgttgcc gtctacccag aaggcgggct tcgtctcggc ggggcccgtc tccaggttgg
    13141 taagctcagc gagccagatc tctctcccct cattgctctt tcctatggac tcgaccttgc
    13201 agaactcggg gtgctcctcc gcgagcgcgt gcatggcccg tgtgagctcc ccgtagcgca
    13261 ggtaagcgtc gaagttcgtc ttcatatcct actccccctc catgtaccga aacccccacg
    13321 atggtcttct tcgacgagcc actagatgtg gcgcgcacgt ttgacatgct ccagcatgtt
    13381 gggtaacgtc agagtcgccc tctggtagtg tacctggagt gtcttcgggc atatatacta
    13441 attgcgttcc ggggtcccca tacggtcgaa ggtggtaaga ggctggaaac gccggttgga
    13501 accgtccttg cagggcgtta caaggtggag agcacgctcg gttccggtgg gatggcggtc
    13561 gtctacaggg ccgaagacga catcctcggt cgtgccgtcg cgctgaagac cctccaccac
    13621 cacttcgccg agatgccctc tttcaggcgt cgcttcaggc aggaggcccg cgcgatggcc
    13681 tccctggatc acgagaacat agtcaaggta tacgacatct cccaggatgg cgaggtgccc
    13741 ttcatcgtcg tcgaatgtgt ctcgggcagg gatctcggtg acctcctcgg tggtagtcgt
    13801 cgccacagcg ggcgtctcaa tgagcagtcc gtaaggagaa tggcgatcca gcttctgcac
    13861 gcgctctcct atgcccacgg gcggggcatc atacacaggg acatcaagcc ttccaatatc
    13921 ctcctcacgt cggagggaac cgtcaaggtc gccgatttcg gcatcgcccg catcgtcgag
    13981 gaggacgatg cgggcgatgc cggtgagatc gtcggcagcg cccgctacat gtcccccgag
    14041 cagctcaggg gcgaggctgc gacaccgagg agcgatgtct actcggtggg tgtcctcctc
    14101 taccactgcc tgacggggag gccgccgttc tcgggcgacg tcaagagcct cgccaggcag
    14161 caaatacaca aagagccgac gccgccgcgc aggctgaaca agaggatcac gccagagatg
    14221 gaggcggtga tcatgaaggc actctccaaa aagcccaggg accggtactt ctcggccgag
    14281 gcgatgctcg acgacataga gagagaatca ccgcccaggc cggcggggac gacagcg
//
LOCUS       DATMVF010000189        22005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_570910, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000189 DATMVF010000000
VERSION     DATMVF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22005
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570910"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1025)
                     /locus_tag="VN178_09300"
     CDS             complement(15..1025)
                     /locus_tag="VN178_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS81207.1"
                     /translation="MEYRSLGRTGVKVSELCLGCMMFGGRTGEEDSADIIDRAIDGGV
                     NFLDTANVYSTGASEEAVGKSLKRNGKRDAVVLATKVHGVMDEDDPNAAGNHRRHIIE
                     QCEASLRRLQVDYIDLYQIHRPSSDVPIDETLRALDDLIRAGKVRYVGTSTYAAWQLV
                     EALWAAKELGLNRFVSEQPPYHLLDRRIERELVPFAQTYGVALIPWSPLAGGFLTGKY
                     SRDESQSPEDSRYGIQPRRMGRNHFTDEAFAILEAVQTIANEKNCTPGQVALAWCKDK
                     PGVTSPIIGPRTMEQLDDNLGAVEVSLDENDNERLDAVAPPGRATVPYYEADFGPHSF
                     RW"
     gene            1197..2189
                     /locus_tag="VN178_09305"
     CDS             1197..2189
                     /locus_tag="VN178_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS81208.1"
                     /translation="MNKVRWGILGTGAIARQFVKGLRSLPEAEVFAVGSRSEDSAVEF
                     ADERNIPRRHASYDALASDPDVDVVYIATPHPFHAENAELCLQAGKAVLCEKPFCVNA
                     AQAERVIELARERGLFIMEGMWTRFIPLMERVRRLLSEGAIGEVRMLNVDFGFRADLD
                     PASRLFERRLGGGALLDVGVYCVSLASMVLGRPSGFVSLPHLGETGVDEQASVVLEHE
                     GGRLANLSIGVRTNTPQEATIMGTEAYIRIHAPWWRPASMTISRPGTEDETVEVPVSG
                     NGFNYEAAEVMRCLEAGKTESDVMPLDETVSVMRTMDGIRAEWGLRYPGEEVAT"
     gene            2186..3178
                     /locus_tag="VN178_09310"
     CDS             2186..3178
                     /locus_tag="VN178_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS81209.1"
                     /translation="MNYGRVPGVDKPVARIVQGTVMIRSEEEGDEERSFALLDEVFAQ
                     GGTTFDTAHQYGGGDCERTLGRWVRDRGLREEVVIIGKGAHHNEDRKRVTPFDITGDL
                     YDSLARLGFDYIDLYLLHRDDPSVPVGPIVEVLNEHKEEGRIHAFGGSNWRPERLREA
                     NDYARKNGLTPMVASSPNFSLAVQVKEPWPDCVSISGDEEGRRWYEETRMPLFTWSSL
                     AGGFFSGRFARNNLDTFDAVLDRICVETYCYEENFGRLDRAGVLAREKGLTVPQVALA
                     YVLDQPLEVFALVGCNTGEEFRANVEAGAVALTREEIAWLAYGDAPSEGQGSSA"
     gene            3223..4194
                     /locus_tag="VN178_09315"
     CDS             3223..4194
                     /locus_tag="VN178_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS81210.1"
                     /translation="MAEAPGVELAAIVDPDPEARRYVDNAPGYGALEEALEGVECEAV
                     LVASPPGTHHVVAKAALEAGKHVLCEKPLATTLQDAFDLVEAAGRAERILMVSQNYRY
                     NAPFRAMQRLVMEGELGELASIKISCRRDTRAQFAPDDFRYSMRHPYVLDMSVHHFDL
                     IRAATGRDVRGVYARSWRVPDSPFVHHPAVAALLDLEGGVPVVYEGDWATHESETSWN
                     GEWEIVGEAGRLLWSGSKEDRGTGKVLLERWGREPRLVGQPDLEFVEREATLQALCAA
                     IEDGEPPETAAADNVKSLAAILGCVRSIESGNAVDVAALLAARGAKL"
     gene            4239..5240
                     /locus_tag="VN178_09320"
     CDS             4239..5240
                     /locus_tag="VN178_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWS81211.1"
                     /translation="MARPVTLFTGQWADLTLEELARKCGEWGFDGLELACWGDHFDVD
                     RALSESGYVEGRWEILRSNGLDCWAISTHLVGQAVCDPIDARHRATLPPDVWGDGDPE
                     GVRQRAARKVMDTARAAARFGVTQVNGFTGSSIWHLLYSFPPNDFAEIERGYEDFAER
                     WNQIIDVFDEEGVRWGLEVHPTEIAYDFVTTRKALDAIGNREGFGINFDPSHFAHQFL
                     DSAAFIEEFADRIYHFHIKDSRKRLNGRRSILGGHLDFGSPERGWDFVSPGHGDVNLE
                     DCIRALNRIGYEGPLSIEWEDSGMDREYGARDALAFARRTDFEPSTQAFDAAFAKEE"
     gene            5243..6415
                     /locus_tag="VN178_09325"
     CDS             5243..6415
                     /locus_tag="VN178_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS81212.1"
                     /translation="MERGEIGVGLVGYKFMGRAHSNAYRQVASFFDVDPAPRMAAICG
                     RDEAGVREAADTLGWDSYETDYRKLIARDDVGLVDVVTPGNTHREIVLTALEAGKHVL
                     CEKPLANTLPEAREMLTAARNAGTVNMVCFNYRRAPAVQLARKLVEEGRLGEIRHWRA
                     TYLQDFIMDPSFPLIWRLQKEVSGSGALGDIGAHIIDLAHFVVGSISEVTGMTKTFVE
                     ERPLEEASAGGGLSASGGTEMGEVTVDDAAAFLARFENGAIGTFEATRFAAGRRNQNS
                     FEVNGSKGSVAFDLERMNELEVFFADDDPDVQGFRTVNVTEPEHPYTGAWWPPGHILG
                     YEHTFIHTMKDLMDGIKAGKSPAPTFEDGYRCQAVLDAVERSAASGEWRRPQTEGI"
     gene            6418..7128
                     /locus_tag="VN178_09330"
     CDS             6418..7128
                     /locus_tag="VN178_09330"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWS81213.1"
                     /translation="MPAEPRVTIWNEYRHEKSDEQVAKIYPEGMHAVLAAALTGAGFE
                     VGTATLDEPEHGLPDEVLDNTDVLLWWGHAAHDEVSDETVEKVHLRVLGGMGLIVLHS
                     GHFSKIFKKLLGTTCDLKWRHDDTERLWVVAPGHPIAEGLGEYIELEEEMYGEPFDVP
                     QPDELVFVSWFGGGEVFRSGLCYRRGRGRIFYFRPGHEENPSYHNRDVQRVIQNAIRW
                     AAPTPGATPVYGHSEPID"
     gene            7165..8280
                     /locus_tag="VN178_09335"
     CDS             7165..8280
                     /locus_tag="VN178_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS81214.1"
                     /translation="MQAERSSAEPLRAGVVGLGYAGEQHLKNFVRVPGVEPVALAGLE
                     EDLLGELGGRYGVSDLYTSWEELVARDDLDIVSIGAPNHLHAPIAIAALEGGRHVLCE
                     KPLARNGAEAERIVSAAREADRAVHIAFTQRERGDVQALKRHVEEGNLGRIYHAKATW
                     MRRNGIPGMGGWFTSKELAGGGPLIDLGVHMVDMAIFLMGEPEVKTVSCATYAELGPR
                     GRGGRGDFGLMQGDKPYEVEDLATAFIRFSGGATLNLEAGWAAYRESSDDFGVTLYGT
                     DGGAEMKVRNYGTSDTVRIYTDVAGVPAVVAPEIAPREGHYAVVRRFIETIRKGAWKG
                     QYGEDGLNRARIIDACYASALENREVTMEEIIREEAV"
     gene            8277..8921
                     /locus_tag="VN178_09340"
     CDS             8277..8921
                     /locus_tag="VN178_09340"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWS81215.1"
                     /translation="MKSALFVCGGWEGHEPEKCAAIFAPILEERGYEVEISTTLDSYL
                     DKEKMRSLDLVIPIWTMGEITDQQEKGLRGAVESGVGLAGWHGGMADSFRHSINYQFM
                     VGGQWVAHPGNIIDYRVNIVDHEDPITSGLEDFSMHSEQYYMHVDPSNEVLATTTFSG
                     EHVPWIEGNTIPVVWKRRWGEGRVFYCSLGHVAKDFDVPEAKELVKRGMLWASR"
     gene            8976..10109
                     /locus_tag="VN178_09345"
     CDS             8976..10109
                     /locus_tag="VN178_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS81216.1"
                     /translation="MEDVTISGDEAVKVGVIGCGKISGIYLENGATFDDVEIVACSDL
                     VLERAEAQAEAYGVPRAYSPEELLADEEVEIVLNLTVPVAHAEVSLAALEAGKHVYTE
                     KPLAVSLEEGRRMLEVAAERDLRIGCAPDTFLGGGLQTCRKIMDEGIIGEPVAVSAFM
                     LSHGPEDWHPNPDFFFQPGAGPMFDMGPYYLTALTTLMGPVRRVAGSARITFPERMIT
                     SEPLSGTTITVNTPTHIAGVMDFESGAVGTLVTTFDVWAESQARIDLYGTEGTLSLPD
                     PNFADGPVRLWRSDAGAWRDVPLTHAYTGNSRGIGLADMAQAVRSGRQIRASGELGMH
                     VLEVIHAFLDSSERGEHVAVSSTFERPEPLPARSPEGIFGGGV"
     gene            10106..10861
                     /locus_tag="VN178_09350"
     CDS             10106..10861
                     /locus_tag="VN178_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWS81217.1"
                     /translation="MRQDQISLQLYTVREETARDMPATLRRISEIGYPAVEFAGFGGL
                     SAEDLSNILDHLGLRASGAHVPFDSWQKDPESVITDMHTLDCTHAILPMAPPEQRRDE
                     SALTTLAGNLNRWGELCRQEGVTFSYHNHDFEFASLDGTTMWDILVGETDPELVYFEL
                     DLYWVRYGGADPGALLSDLGDRIPLVHLKDMASGEQRSDLPVGEGTMPWKGLLRAADA
                     AGVQWYVAEQDNPRDALEDVRISLQSMRELASG"
     gene            10907..11098
                     /locus_tag="VN178_09355"
     CDS             10907..11098
                     /locus_tag="VN178_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81218.1"
                     /translation="MSKDSPQKNSGCEFWGWILFTISALFFIASSLRNGDMIGLLGGV
                     FFLLACAAFLAAYFGTHED"
     gene            complement(11147..11752)
                     /locus_tag="VN178_09360"
     CDS             complement(11147..11752)
                     /locus_tag="VN178_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HWS81219.1"
                     /translation="MNILDTRVYRWLETGTDFFLLNLMWLVACLPVVTIFPSTAAMFG
                     VVRDWVRGKEGSLTRTFIARFRENLGQSLLVGTIWTVFGVALFLDFLVASQLSYWAEI
                     VLKSVLVLVSTVYAFGSVYLFPVMVHYEADWKTIIKNSLLISVGRLPTTLACLVFLVV
                     MVGLTVVVPFLIAITGSFTAYVVYKLCDRVFRQIDAAHGDA"
     gene            complement(11779..12873)
                     /locus_tag="VN178_09365"
     CDS             complement(11779..12873)
                     /locus_tag="VN178_09365"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWS81220.1"
                     /translation="MVTSTTRSVSVDEYKTFRQQGFLIVRGLLGSDEVEELREHTELL
                     MQGRLPEQVGTMSERDTNSDTGTTGQTLEAPPEHLSPEEKAQYFLRVHMLHRKLELHE
                     RYLLHPGVLDVLEALIGPDVLALQTMLFLKPPGKPGQGWHQDSYYIPTHPDTLCGAWF
                     AIDDADELNGAMWMAAGSQHEPVYPPKEGYGHGDGRLEGIEHISGASDPDDESNDLSR
                     IADRYPQLLAPLEAGDVVFFGGHVLHRSKQNFTTDRLRRAFVGHYCNARSFTQWGANH
                     DDNDPHTAPSVDPETKMTNGSHILARGDTHLPFAKPTFGTPCAALLPAEERRKESEYA
                     AAMMADMDSDDMGARPADPEAEHDHADGQD"
     gene            13196..14527
                     /locus_tag="VN178_09370"
     CDS             13196..14527
                     /locus_tag="VN178_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS81221.1"
                     /translation="MKSSDRLTRRDFLKMSGAGVAGVGLLGIYGCGRGGGSSTGQVDW
                     ASWANSGEAERFREYTDHFMEEYPNIQVTYTPTPNYDDYHPKILTQLNGGTAPDAFYA
                     GDIWVAQFIKNGTISKLNNLMTGPNSEEKPDAFAAGLWGPAKTSEGTIYGITVDCNPT
                     LIWYNKGVLQDANISEDPMDLYENGEWKWDVFVQMNEQVASKGGTNGFMFENGNNWFY
                     SWCRQNGGTPYEGDLCIVNEDAKSVEAFQWMYDNIRSGAFTYAGNLPTGQTSDAQFNS
                     SRTAFMEAGRWVLPVYKQNSSLKYDVVPFPTNTDAKMEPGWVATAYMVMNKKTASVKD
                     TFTFITNFTSSAGQKFRLEEAGNAVPSVLEGADGVVTKDTTLEGAEYFLDIRNTGLVG
                     YAELTVPGLVEDMQDVFEKRLWLPSGDGDVQGTLDEVAKMANEGIKKQSIE"
     gene            14610..15518
                     /locus_tag="VN178_09375"
     CDS             14610..15518
                     /locus_tag="VN178_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS81222.1"
                     /translation="MSSLVKASKLSQRRKSDIRWGYFFIFPQLFGVLAFVLFPLVYVL
                     VLSLLEWNGLSEATFVGLSNYSTELTSSEFRNALTNTVYYTVMVVPSSVVLSLMAALA
                     LNKLRLKNFFRVIYFAPVVTSAVAAGVVWLWVYNPDFGLINSYLQNWFGIKGPNWLLD
                     PKWVMPSIAIMSIWLSMGFYIVIFLAGLQGIPAAYLEAAQIDGANRFQRFWYVTLPLL
                     SPTTFFVFIIAIINSFQVFDQAFIMTAEGGPANSAVTMVYYLRDLAFVDFVYGRSAAV
                     AVILFAIILAFTLLQFRVQRQWVHYE"
     gene            15546..16427
                     /locus_tag="VN178_09380"
     CDS             15546..16427
                     /locus_tag="VN178_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS81223.1"
                     /translation="MASVATTKDVSGPVESTSRTGRLVASVLLYIAVSLGAVVMIMPF
                     AWMLLASLKDLGQIYQVPPNWIPDPFVFQNYVNAWTAVPFATGYINSAIVTVTVVVAN
                     LLTCSMAAYAFARIEFPFRHALFILFLATLMVPEQVTIIPLYIIIESLGLIDTLLSLI
                     IPYALFNAFGVFLLRQFIKSLPVDLEEAAIVDGANRWTIYWRIIVPLIRPALAAFGIF
                     TFLFQWNNFFRPLVFLNSIDNYTVPLAINFFRGQYATDFALLMAGSAISIVPVLLVYV
                     IGQRQIIEGIATTGLKQ"
     gene            16464..17999
                     /locus_tag="VN178_09385"
     CDS             16464..17999
                     /locus_tag="VN178_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HWS81224.1"
                     /translation="MPTSDGYIDTHLREPLVEENAFVRMSPEIAPPPSFEDAWDSLPR
                     TFWEGHEGVIDCYRRAWELAFANVRRPEPESGFVASFIDTAFNDCLFMWDSAFILLFA
                     RYGRRAFDFQRTLDNLYASQHPDGFICREIDTFDGKDRFERFDAASTGPNVMPWTEWE
                     HYSDTADSERLGLVFPVLVSYHRWLRRHRTWRDGTYWTSGLGSGMDNQPRFPQRPSGD
                     AGWPERLTYHGHAVWIDACLQQILSADLLLRMAEALGRLDEVADLREERDRLSLTVNE
                     RLWNEETAFCHDELADGTLSDVKTVGAFWALLAGVVPKERISSFVGHLEDPKRFGRPH
                     RVPSLSADHPEYDPDGGYWLGGVWPPTNYMVLRGLDRAGYNDLAHEISREDLDHVVGV
                     FEQTGTLWENYAPERTAPGNRAKDDFVGWSGLAPVAGLFEYVFGLRADAPSRRLLWDV
                     RLLERHGVYRYPFGRDGVLDLSCATRSSATEEPKIQVSSTVSLELVIRWEGGERTVAV
                     PEG"
     gene            18061..18867
                     /locus_tag="VN178_09390"
     CDS             18061..18867
                     /locus_tag="VN178_09390"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWS81225.1"
                     /translation="METTKHLTREQIQGFQEDGYLVVESLFAPEEVKALVDNFMQMHA
                     EGPIPGCFEPADPEEADGDILKLYPRIMHPHRVNELALRYLLDDRLEDILADLFGEEP
                     LAAQSMLYFKPAGAKGQALHQDNFYLKVEPGTCIAAWVALDAADQENGGLEVVPGTHD
                     MELFCPEEADTDVSFTRDYVPVPEGLEAVPVVMRPGDVLFFNGQLVHGSQPNSSEDRF
                     RRSFICHYISRFSERVSKWYRPILTMDGEEVRIEESAGGGPCGTEDAGVH"
     gene            18945..20267
                     /locus_tag="VN178_09395"
     CDS             18945..20267
                     /locus_tag="VN178_09395"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HWS81226.1"
                     /translation="MPKITLIGAGSAVFTRNLCSDILLAPALQESMISLMDIDSDRLR
                     TSRDLVQNIVDRRGLDARVEATTDRREAIEGADYVVTTFQQGGLDAYALDIEIPQRYG
                     VEQCVGDTLGPGGVFRALRTIPVLLDLCDEMDELAPNAQLLNYVNPMAANCWAVDVGS
                     GRPHVGLCHSVQGTGEMLASWIGVPYEEVNFLCAGINHQAFFLEFRSGRDDLYPEIRD
                     AIRRDDIRGQEPVRVEIMEHFGYFVTESSGHASEYAPYFRKDARMVEKELVPRFVDEA
                     DHWFDFGRTGGYLRHCLSRLEEPPDGHGPLPTDRTHEYGSYIIEAMETDEPIKINGNV
                     PNRGLMDSLPNRCCVEVPCLVDANGVQPTVIGHYPPQLAALNRTNINVQELTVEAALS
                     GDVEAVHHAVMLDPLTAAVCTLPQIRSMVDEMLEAQAKWLPQFTEITA"
     gene            20274..>22005
                     /locus_tag="VN178_09400"
     CDS             20274..>22005
                     /locus_tag="VN178_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HWS81227.1"
                     /translation="MDRTRVKAGGIEVRLDGGEKGFRSSLSTSALESGVELVHLRIES
                     GDVARPPVFRLSWFYPLVSVHGFWHSGAGYDKGLGVDWGKGFYSKATSGAPVGCLYDL
                     KGRNRLTFALSDTLDPVTFHAGVHEESGEARCWVRFFDEPPAPLSTYETTLRLDTRDL
                     PYHQVLSDVSRWWAGLPGYEPAGVPETARLPMYSTWYSFHQDLDPGRVEEQCRLAREL
                     GCEAVIVDDGWQTTSNERGYAYTGDWEPAPEKVPDMRAHVDRVHALGMKVLLWYSVPF
                     VGRHSKVWERFSGMLLEEIERHGAGVLDPRFPEVREFLIGTYESALREWDLDGLKLDF
                     VDSFGASKEAGGGRDYDGVPEAVDRLLEDTIVRLRRIKPDVMVEFRQSYVGPLMRKYG
                     NMFRAMDCPNDAVENRMHTLDIRLLCGDTAAHSDMLMWHTEEPVQSAALQLVNVLFSV
                     PQISVLLDRIPPEHLRMLRFWLGFWREHRDVLLDGALEPLHPEAIYPVVLARTESKLA
                     AVAYGNAVVPLEGEIPSTLFVVNGTLEEGVVLSLADDAGTRGIEVRDCRGQVTRTDDF
                     GLETGLCRIDI"
BASE COUNT         4251 a         6797 c         7033 g         3924 t
ORIGIN      
        1 aaacccgccc gctctcacca acggaacgaa tgcgggccga aatccgcctc gtagtagggg
       61 acggtggccc gtcccggggg cgcgacggcg tcgagtcgct cgttgtcgtt ctcgtcgagc
      121 gagacttcga cggcgcctaa gttgtcgtcg agttgttcca tggtgcgcgg cccgatgatg
      181 gggctcgtaa cgccaggctt gtctttgcac caggccaggg ccacctgccc cggagtgcag
      241 ttcttctcat ttgctattgt ctggaccgct tcgaggatag cgaacgcttc gtccgtaaag
      301 tggttgcggc ccatgcgcct cggttgaatg ccgtagcggc tatcctcggg tgactggctc
      361 tcgtcgcggc tgtactttcc ggtgaggaac ccgccggcga gcggagacca ggggatcagg
      421 gcgaccccgt aagtctgcgc gaaaggaaca agctcgcgct cgatgcgccg gtcgaggagg
      481 tggtaggggg gctgctcgct cacaaagcgg ttcagcccga gctccttcgc ggcccacaag
      541 gcttcgacga gttgccaggc cgcgtaggtg gatgtgccaa cgtaacgcac cttgccggcg
      601 cggatcaggt cgtcgagcgc ccgcaacgtc tcgtcgatgg gtacgtcaga gctggggcgg
      661 tggatctggt aaagatctat gtagtccacc tgaagacgcc tcagcgaggc ctcgcactgc
      721 tcgataatgt ggcgcctgtg gttaccagcg gcgttcggat cgtcctcgtc cataacaccg
      781 tgcaccttgg tcgcgagcac gacggcgtcg cgctttccgt tccgctttag cgacttccct
      841 acggcctcct cgctggcgcc ggtgctgtag acattcgccg tatcgaggaa gttgacaccc
      901 ccgtcgatgg cgcggtcgat gatgtctgcg gagtcttctt ctcccgtgcg gccaccgaac
      961 atcatgcacc cgaggcagag ctcgctgacc ttcacgcccg ttcttccgag gctcctgtac
     1021 tccacgcttc actccttcga ggtagacgta cacccatgca agttagacgt atacccaatc
     1081 gatgtccttc gtaactccct tgcaacctcc gcgtttgatt gtgctcacct tagtgtgtag
     1141 catcgcggtt tgtgcatctg gccgtgtgag gtgggaaggg aaggcgcgca gagatcgtga
     1201 acaaggtccg gtgggggata ctcgggacgg gcgccatcgc gaggcagttc gtaaaaggcc
     1261 tgcgctccct gcccgaggcc gaggtgttcg ccgtcggctc gcgctcggaa gactcggcgg
     1321 tggagttcgc cgatgagagg aacatcccac gccgccacgc gagctacgat gccctcgcct
     1381 cagacccaga cgtcgacgtc gtctacatcg caacacccca ccctttccat gcggagaacg
     1441 ccgaactatg ccttcaggcc gggaaggccg tgctctgcga aaagcccttc tgcgtgaacg
     1501 ccgcccaagc cgaacgggtg atcgagctcg ccagggaaag aggactcttc atcatggaag
     1561 gcatgtggac caggttcatc cccttgatgg aaagggtgcg gcggctcctc tccgaagggg
     1621 ccataggcga ggtgcggatg ttgaacgtgg acttcggatt cagggcggat ctcgaccccg
     1681 cttcccggct cttcgaaagg aggctcggcg ggggagccct gctcgacgtc ggggtctact
     1741 gcgtctcgct cgcttccatg gtcctcggac ggccttcggg gtttgtgagc ctgccgcacc
     1801 tcggggagac gggcgtcgac gagcaggctt ccgtagtgct cgagcatgaa ggcggaaggc
     1861 tcgccaacct gtccataggt gtcaggacga atacacccca ggaggcgacc atcatgggaa
     1921 ctgaagccta tatcaggatc cacgccccct ggtggcgccc tgcgtcgatg accatctcca
     1981 gacccggcac agaggatgag accgtcgagg tcccggtctc cggcaacggc ttcaactatg
     2041 aggctgccga ggtcatgcgc tgcctggagg caggcaagac ggagagcgat gtaatgccat
     2101 tggacgagac ggtctccgtc atgaggacga tggacggcat aagggccgag tgggggctca
     2161 ggtaccccgg cgaggaggtc gcgacttgaa ctacggacgg gtacccggcg tagataagcc
     2221 ggtggcgcgg atcgtgcagg gcaccgtaat gatccgttcc gaagaggaag gggacgagga
     2281 gcggagcttc gcgctcctcg atgaggtctt cgcccagggc ggcaccacgt tcgatacggc
     2341 ccaccagtac ggcgggggcg actgtgagcg gaccctcggc cggtgggtaa gagaccgcgg
     2401 cctgcgcgag gaggtcgtaa taatcggcaa gggcgcccat cacaacgagg accgcaagcg
     2461 ggttaccccc ttcgacatca cgggagacct ctatgactcc ctggcccgtc tggggttcga
     2521 ctacatagac ctctacctgc tccaccgcga cgacccatcc gttcccgtcg gccccatcgt
     2581 ggaggtcctc aacgagcaca aagaggaggg caggatccat gccttcggag gctcgaactg
     2641 gagacccgag cgcctgcgcg aagcgaacga ctacgccaga aagaacggcc tcacacccat
     2701 ggtcgccagc agccccaact tcagcctcgc cgtgcaggtc aaagagccct ggcccgactg
     2761 cgtgagcatc agcggcgacg aggagggccg ccgttggtat gaggagacca ggatgccgct
     2821 ttttacctgg tcgagcctcg ctggcggatt cttctccggc cgctttgccc gcaacaacct
     2881 ggacactttc gacgcggtcc tcgacaggat ctgtgtcgag acctactgct acgaggagaa
     2941 cttcgggcgc ctcgaccgcg ccggcgttct cgccagggag aaagggctga ccgtgccgca
     3001 ggtagccctc gcttacgtcc tggatcagcc cctcgaggtc ttcgccctcg tcggctgcaa
     3061 cacgggcgag gagttcaggg ccaacgtcga ggccggcgcc gtagcgctga cccgggaaga
     3121 gatcgcctgg ctcgcgtacg gcgacgcacc atcggagggg caaggatcgt ccgcgtagtc
     3181 caggcagggc tcggtatctg gggacgtagc tggtccgggc tggtggccga agcccccggc
     3241 gtggagctcg ccgccatcgt cgatcccgac cccgaagccc gccgttatgt agataacgcc
     3301 cctggctacg gagccctgga ggaggcgctg gagggcgtcg agtgtgaggc ggtgctggtc
     3361 gcttcgccgc cgggcacgca ccacgtggtg gcgaaggccg ccctggaggc aggcaagcat
     3421 gtcctctgtg agaagcctct ggcgaccacc ctccaggacg ccttcgacct cgtcgaggcc
     3481 gcaggtaggg cggagcgcat cctcatggtc agccagaact accgttacaa tgcgccgttc
     3541 cgtgcgatgc agcggctcgt gatggaaggc gagttagggg agctagcgtc gatcaagata
     3601 tcttgccggc gcgacacgcg ggcgcaattc gccccggacg atttcaggta ctccatgcgc
     3661 cacccttacg tcctcgacat gtctgtccac cacttcgacc tcataagggc cgcgaccgga
     3721 cgtgacgtgc gcggcgtgta cgcccgtagc tggcgcgtac ccgacagccc gttcgtccac
     3781 cacccggcgg tagccgccct gttggacctg gaaggcggcg tgccggtcgt ctacgaggga
     3841 gactgggcga cccacgagtc cgagacctca tggaacggtg aatgggagat cgtcggtgag
     3901 gccggaaggc tactctggag cggcagcaaa gaggaccgtg ggaccggcaa ggtgctcctc
     3961 gagcggtggg gccgggagcc gcgtctcgtc gggcaacccg acctcgagtt cgtggagagg
     4021 gaggcgacgt tgcaggcgct gtgcgcggcg atcgaggacg gcgagccgcc ggagaccgcc
     4081 gccgcggaca acgtgaagag cctggccgcc atacttggtt gcgtaaggtc catcgagagc
     4141 gggaacgctg tagacgtggc cgcgctcctg gccgcgaggg gcgctaagct gtaggggccg
     4201 atccgcggcg agtagaaaga gtaggaggag gttggaacat ggccagaccg gtaacgctct
     4261 ttacgggaca gtgggctgac ctgacgctgg aagagctcgc caggaagtgc ggcgagtggg
     4321 gtttcgacgg gcttgagctc gcgtgctggg gggatcactt cgacgtagac cgagcgctct
     4381 cggagagcgg ttacgtcgag ggacgctggg agatactgcg cagcaacggc cttgactgct
     4441 gggccataag cacccacctg gtcggccagg cggtctgcga ccccatagac gcccgccacc
     4501 gggcgacgct ccctcccgac gtgtggggcg acggcgaccc ggagggcgtg cgccagcgag
     4561 ccgcccggaa ggtcatggac acggccaggg ccgctgcccg ctttggggta acgcaggtca
     4621 acggctttac cgggtcgagc atctggcacc tgctctactc gttcccgccc aacgacttcg
     4681 cggagataga acgcggctac gaggacttcg ccgaacggtg gaaccagatc atcgacgtct
     4741 tcgacgagga gggcgtcagg tggggcctcg aggtgcatcc cacggagatc gcctacgact
     4801 tcgtgaccac ccgcaaggct ctcgacgcca tcggcaatcg cgaggggttc ggtatcaact
     4861 tcgatccgag ccacttcgcc caccagttcc tggattccgc cgcgttcatc gaggagttcg
     4921 cggataggat ctaccacttc cacatcaagg acagccgcaa gcgcttgaac ggccgcagga
     4981 gcatactggg aggccacctg gacttcggca gtcctgagcg tggctgggac ttcgtctccc
     5041 cgggccacgg cgacgttaac ctggaggact gcataagagc gctgaaccgt attggctatg
     5101 agggccccct gagcatcgag tgggaagact cggggatgga ccgggagtac ggggccaggg
     5161 acgcgctggc tttcgcgaga cgtacggatt tcgagccgtc gacgcaggcc ttcgacgctg
     5221 ccttcgcgaa ggaggagtag agatggagcg gggagagatc ggggtcgggc tagtcggcta
     5281 caagttcatg ggccgcgccc actccaacgc ctaccgtcag gtcgccagct tcttcgacgt
     5341 cgacccggcc cccaggatgg ccgcgatctg tggccgcgac gaggccggcg tgagggaggc
     5401 ggcggacacc ctgggctggg actcctacga gaccgactac cgcaagctca tcgcgcgcga
     5461 tgatgtaggc ctcgtggacg tcgtaacacc gggcaacacg caccgcgaga tcgtgctcac
     5521 agccctcgaa gcgggcaaac acgtcttgtg cgaaaagccg ctcgccaaca ccctccccga
     5581 ggcgcgcgag atgctcaccg cggcacgtaa cgcggggacg gtcaacatgg tctgcttcaa
     5641 ctacaggcgc gcccccgcgg tgcagttagc gcggaagctc gtcgaggagg ggcggctcgg
     5701 ggagataagg cactggcggg ccacgtatct gcaggacttc atcatggacc cttctttccc
     5761 cctgatctgg cgcctccaga aagaggtctc cggctcaggc gccctcggcg acataggggc
     5821 gcacataatc gacctggccc acttcgtggt cgggtccatc tccgaggtca cgggcatgac
     5881 gaagacgttc gtagaggagc ggcccctcga ggaagcgagc gccggcgggg gactctcggc
     5941 gagcggtggg accgagatgg gcgaggtgac cgtagacgac gccgcggcct tcctggcccg
     6001 cttcgagaac ggggccatag ggaccttcga ggccacgcgc ttcgcggccg gccgcagaaa
     6061 ccagaactcc ttcgaggtca acggctccaa aggaagcgtc gccttcgacc tggagcgcat
     6121 gaacgagctc gaggtgttct ttgccgatga cgaccccgac gtccagggct tcaggaccgt
     6181 gaacgtgacg gagcctgagc atccgtacac gggggcctgg tggccgccgg ggcatatcct
     6241 gggctacgag cacaccttca tccacaccat gaaagacctg atggacggaa taaaggcagg
     6301 gaagagtccg gccccgacct tcgaggacgg ctaccggtgc caggccgtgc tcgacgccgt
     6361 cgaacgcagc gccgcgagcg gtgaatggag aagaccacag acggagggga tctagacttg
     6421 cctgcagagc cgcgcgtaac catctggaac gagtacaggc acgaaaagag cgacgagcag
     6481 gtcgcgaaga tctaccccga aggcatgcac gccgtcctag ccgcagcact caccggggcc
     6541 ggcttcgagg tcggcactgc taccctcgac gagcccgagc acgggctacc cgatgaggta
     6601 ctggacaaca cggacgtgct cctctggtgg ggtcatgcgg cccacgatga ggtctccgac
     6661 gaaacggtag agaaggtgca cctgcgggtg ctcggcggga tgggcctgat cgtgctccat
     6721 tcaggacact tctcgaagat cttcaagaag cttttgggca ctacctgcga cctcaagtgg
     6781 cgccacgacg acaccgagag gctctgggtc gtcgcgccgg ggcaccccat cgcggagggg
     6841 ctcggggagt acatagagct cgaagaggag atgtacggcg agccattcga cgtgccgcag
     6901 ccggacgaac tcgttttcgt gagctggttc gggggtgggg aggtcttcag gagcggcctg
     6961 tgttacagga gggggcgggg taggatcttc tactttcgcc cgggccacga ggagaacccc
     7021 tcctaccaca accgggatgt ccagcgcgtc atccagaacg cgatccgctg ggccgcgccg
     7081 acaccagggg cgacgcctgt atacgggcac tcagaaccga tagactaggg tcgaagagat
     7141 ctagacccac aagaggggag ccttttgcaa gctgagagat cttctgcgga acccctgcgc
     7201 gccggggtgg tcggcctcgg ttacgcgggc gagcagcact tgaagaactt cgtgagggtg
     7261 cctggcgtgg agcccgtggc gctcgccggt ctcgaagagg accttctggg cgagctcggc
     7321 gggcgctacg gcgtgtcgga cctgtacacg agctgggagg aactcgtcgc ccgcgacgac
     7381 ctcgacatcg tgagcatcgg ggctccgaac cacctgcacg cccccatcgc catagccgcc
     7441 ctcgaaggcg gcaggcacgt gctctgcgag aagccgctgg cgcgcaacgg cgctgaggcg
     7501 gagcggatag taagcgcggc cagggaggcg gatcgcgcgg tgcacatcgc cttcacccag
     7561 cgcgagcggg gcgacgtcca ggcgctcaag cgtcacgtcg aagaagggaa cctgggcagg
     7621 atctaccacg ccaaggctac ctggatgcgc cgcaacggca tccccggcat gggcggatgg
     7681 ttcaccagca aggagctggc cggcggaggc ccgctcatag acctcggcgt gcacatggtg
     7741 gacatggcca tcttccttat gggcgagcct gaggtgaaga ccgtaagttg cgccacttac
     7801 gccgagctcg gcccgcgcgg tcggggtggc aggggcgact tcgggctcat gcagggcgac
     7861 aagccatacg aggtcgagga cctggcgaca gcctttatac gcttctccgg tggggcgacg
     7921 ctgaacctgg aggccggctg ggccgcctac cgcgagtcga gcgacgactt cggcgtcacc
     7981 ctttacggca ccgacggtgg ggcagagatg aaggtgagga actacggcac cagcgatacc
     8041 gtccggatct acaccgatgt ggccggcgtg cccgccgtcg tagcccccga gatcgcaccc
     8101 cgcgaaggac actacgccgt cgtcaggcgc tttatcgaga cgatccggaa aggcgcctgg
     8161 aagggccagt acggggagga cggcctgaac agggcccgca tcatagacgc ctgctacgcc
     8221 tcggccctcg aaaaccgtga ggtcaccatg gaggagatca tccgcgagga agccgtatga
     8281 agtccgcgct cttcgtctgc ggcggctggg agggccacga acccgagaag tgcgctgcta
     8341 tcttcgcccc tatcctggag gagagaggat acgaagtgga gatctccacc accctcgact
     8401 cctacctgga caaggagaag atgagatccc tcgacctcgt aatcccgatc tggacgatgg
     8461 gggagatcac agaccagcaa gagaagggcc tgcgtggggc cgtggagagc ggggtcgggc
     8521 tcgccggctg gcacggcggc atggccgact cgttccgcca cagcatcaac taccagttca
     8581 tggtgggagg ccagtgggtc gcccatcccg gcaacataat cgactaccgg gtcaacatcg
     8641 tagaccacga agaccccata acctcgggcc tcgaggactt cagcatgcac tccgagcaat
     8701 actacatgca cgtagacccc tccaacgaag ttctggcgac caccaccttc tcgggggagc
     8761 acgtaccctg gatcgagggc aacacaatac ccgtcgtctg gaagagacgg tggggcgaag
     8821 gccgcgtgtt ctactgctcc ctgggccacg tggcgaagga cttcgacgtg ccggaagcca
     8881 aagagttagt caaacgcggc atgctctggg cgagcagatg agcaaggcgt caggtttcag
     8941 gtttttcctg atgcctgtag cctgaaacct acaaaatgga ggacgttacc attagcggag
     9001 atgaggcagt caaagtcgga gtaatcgggt gcggcaagat cagtggcatc tacctggaga
     9061 acggggccac gttcgacgac gtggagatcg tggcctgttc ggacctcgtg ctggagaggg
     9121 ctgaagccca ggcggaagct tacggtgtac cgagggctta cagcccggag gagctgctgg
     9181 ccgacgaaga ggtcgagatc gtcctcaact tgacggtccc cgtcgcgcac gccgaggttt
     9241 cgctggcggc cctggaggcc ggcaagcacg tttacaccga gaagcctctc gccgtgagcc
     9301 tggaggaagg gaggaggatg ctggaagtgg ccgccgagag ggacctcagg atcggctgcg
     9361 ccccggacac cttcctcggt ggcggcctcc agacctgccg caagatcatg gacgagggga
     9421 tcatcggtga gcccgtcgcc gtatcggcgt tcatgctaag tcacggcccg gaagactggc
     9481 atcctaatcc tgacttcttc ttccaacccg gcgccggccc gatgttcgac atggggccct
     9541 actacttgac agcgctcacc accctgatgg gacccgtgag gagggtcgcc gggtcggccc
     9601 gcatcacgtt tccggagcga atgatcacca gcgaacctct ctcggggacc accatcacgg
     9661 tgaacacccc gacccacatc gccggcgtga tggacttcga gagcggcgcc gtggggaccc
     9721 tggtcaccac cttcgacgtc tgggcggagt ctcaggccag gatcgacctc tacggtaccg
     9781 agggcaccct gagcctaccc gaccccaact tcgccgacgg tcccgtacgc ctctggcgct
     9841 cggacgcagg cgcctggagg gatgtgcccc tgactcatgc ttacacgggg aacagcaggg
     9901 gaataggcct cgccgacatg gcccaggccg ttcgctcggg cagacagatc agggccagtg
     9961 gtgaactcgg catgcacgtc ctggaggtga tccacgcttt cctggactca tccgaacggg
    10021 gcgagcacgt cgctgtgagc agcaccttcg agcgaccgga accgcttccg gccagatccc
    10081 ccgaggggat ctttggaggt ggagtatgag gcaggaccag atctcactcc agctctacac
    10141 cgtccgcgaa gagaccgccc gcgacatgcc ggccacgctg cgccggatct cggagatcgg
    10201 gtacccggcc gtcgagttcg caggtttcgg cggtctctcc gccgaagacc tcagcaacat
    10261 cctcgaccac ctcggtctcc gcgcctccgg cgcccacgtc cccttcgact cctggcagaa
    10321 ggacccagag tcagtcatca cagatatgca caccctcgac tgcacccacg ccatcctccc
    10381 catggccccg ccggagcaac gcagggatga gagtgcgcta accactctgg ccgggaacct
    10441 gaaccggtgg ggggagttgt gccgccagga gggcgtgacc ttctcctacc acaaccacga
    10501 cttcgagttc gcgtccctgg acggtaccac gatgtgggac attctcgtgg gggagactga
    10561 ccctgagctc gtctacttcg agttggatct ctactgggtc cgctacggtg gcgcagatcc
    10621 aggggcgctt ctctcggatc tcggtgaccg cataccgctc gtccatctga aggatatggc
    10681 ttcaggcgag caacgctccg acctcccggt aggagaagga accatgccgt ggaaagggct
    10741 attgagagct gccgatgcag ccggggtaca atggtacgta gccgagcagg acaacccgag
    10801 agacgctctc gaagacgtaa ggatcagcct gcaatccatg cgagagctgg caagcgggta
    10861 ggaacttcag cgacccgcaa gcacattagg gtgccgagag gcatacgtgt ccaaagacag
    10921 cccacagaaa aacagtggat gcgagttctg gggatggatc ctcttcacca tctccgcact
    10981 gttctttata gcgtcgagct tgcggaacgg agacatgatc ggcctgctag gcggggtgtt
    11041 ctttctcctc gcctgtgcgg cgttcctcgc tgcttacttc ggtactcacg aggactaatg
    11101 tcgagttggt tggtactggc ccgtgttcgg gcaggacaag ccctggctac gcgtctccgt
    11161 gggccgcgtc tatctgcctg aacaccctgt cgcaaagctt gtagaccacg taggctgtga
    11221 agctgcccgt gatggctatc aggaaaggca ccaccacggt gagcccaacc ataactacca
    11281 ggaataccag gcacgccagg gtggtcggta gccttcctac agatatcagc agagagttct
    11341 tgatgatcgt cttccagtcc gcctcgtagt ggaccatcac ggggaacaga tacacagaac
    11401 cgaaagcgta cacggtggag acgagcacga gtaccgactt cagcacgatc tcggcccagt
    11461 aggagagctg gctggccacc aggaagtcga ggaagagagc cactccgaac accgtccaga
    11521 tcgttcccac caggaggctc tgccccaggt tctcccgaaa gcgcgctatg aacgtacgcg
    11581 tcaggctgcc ttcctttccg cgcacccagt cccgcaccac accaaacatg gcggctgtgg
    11641 aggggaagat cgtcaccaca ggcaggcagg ccacgagcca catcaggttg agcaggaaga
    11701 agtccgtgcc cgtctccagc cacctgtaga ccctagtgtc cagtatgttc aaagccggtc
    11761 gcctgctttc acagctgttc agtcttggcc gtcggcatga tcgtgttccg cttcggggtc
    11821 cgcgggtctg gcccccatgt cgtcgctgtc catatcggcc atcatggcgg ctgcgtactc
    11881 gctctcctta cgccgttcct cggccggcag aagggccgcg cacggggtac cgaaggtcgg
    11941 tttcgcgaaa ggaagatgcg tgtccccgcg ggcgaggatg tgagacccgt tcgtcatctt
    12001 tgtttcgggg tcgacgctgg gggcggtgtg cgggtcgtta tcgtcgtggt tcgcgcccca
    12061 ctgggtgaac gagcgggcgt tgcagtagtg gccgacgaag gcgcgccgca agcggtcggt
    12121 ggtgaagttc tgtttgctgc ggtgaagcac gtgtccgccg aagaagacca cgtctcctgc
    12181 ctccagaggc gcgagtagct gcgggtagcg gtcggcgatc ctgctgagat cgttgctctc
    12241 gtcgtcgggg tcgctcgccc cgctgatgtg ctctatccct tcgagccgtc cgtcgccgtg
    12301 accgtagcct tcctttggcg ggtagacggg ctcgtgctgg ctccccgcgg ccatccacat
    12361 cgcgccgttc aactcgtcgg cgtcgtctat ggcgaaccag gccccgcaca gcgtgtcggg
    12421 gtgggtcggg atgtagtagg aatcctggtg ccagccctgc ccgggcttgc ctgggggctt
    12481 gaggaaaagc atcgtctgga gcgccagaac gtcgggcccg atcagggcct ccagcacgtc
    12541 cagcacccca ggatggagca gatatcgttc gtgcaattcc agcttgcggt gcagcatgtg
    12601 tacccgcaag aagtattgag ccttctcctc gggcgagaga tgttccggcg gcgcctcaag
    12661 ggtttgcccc gtagtgcctg tatcagagtt cgtgtccctc tccgacatcg tccccacctg
    12721 ctcgggcaac cgtccctgca tcaggagctc cgtgtgctcg cgcaactcct ccacttcatc
    12781 ggaacccaag agtccgcgga ctatcaggaa accctgttgc ctgaaggtct tgtactcatc
    12841 gaccgaaacg gatctcgtcg tgctcgtaac cacgcctcac tccttcacat gggtctcgtc
    12901 gcccgaaaac cgaactcgag gtcagcaatc atctacttca accatatcag cagcatcctc
    12961 cggaaacaat cgttccaaga tccagcttgt gagcgatcct ggaacgggtg ccggatcgcc
    13021 tcgcgttcga cgatgcattc ggcaaagagc gaaaacaaag agctgacaag ctgagatgct
    13081 gacgagcgaa ggctaaaagc ctgagcgtgc tgattgtgcc gcgttgaccc gctatgtgtg
    13141 acgatgtagg attgaaaaga tccaacacaa cccaggggga aagggcaaat acgggatgaa
    13201 gagttctgac cggcttacgc ggcgggattt cctgaagatg agtggcgccg gggtggcggg
    13261 cgtcgggctg ctcggcatct acgggtgcgg gaggggtggt ggctcctcca cggggcaggt
    13321 cgattgggcc tcatgggcca acagcggcga ggcggagcgt ttcagggagt acaccgacca
    13381 tttcatggag gagtatccca acatccaggt cacctacact ccaaccccta actacgacga
    13441 ttaccatccc aagatcctga cccagctcaa cggcggcacc gcgcccgacg cgttctacgc
    13501 cggcgacata tgggtcgccc agttcataaa gaacgggacc ataagcaagc tcaacaacct
    13561 catgacggga cccaatagcg aggagaagcc ggacgcgttc gccgccgggt tgtggggccc
    13621 tgcaaaaacg agcgaaggga ccatctacgg tattaccgtc gactgtaacc ccaccctcat
    13681 ctggtacaac aaaggggtac tccaggacgc gaacatcagc gaagatccca tggacctcta
    13741 cgagaacggt gagtggaagt gggacgtctt cgtgcagatg aacgagcagg tcgcctcgaa
    13801 gggcggcacc aacggattca tgttcgagaa cggcaacaac tggttctact cgtggtgcag
    13861 gcagaacggt gggacgccgt acgaaggcga cctctgcatc gtcaacgagg acgccaaatc
    13921 ggtcgaggct ttccagtgga tgtacgacaa catcaggtca ggagccttca cctacgccgg
    13981 gaacctgccc acagggcaga cttccgacgc ccagttcaat tccagccgca cggcctttat
    14041 ggaggccggc cggtgggtcc tgcccgtcta caaacagaac agctctctca agtacgatgt
    14101 ggtgcctttc cccaccaaca cagacgccaa gatggagccc ggatgggtgg ccactgcgta
    14161 catggttatg aacaagaaga ccgctagcgt gaaggacacg ttcacattca tcaccaactt
    14221 cacttcttct gccgggcaaa aattcaggct ggaggaggcg ggtaacgccg tgccttcggt
    14281 gctcgaaggt gccgacggcg tcgtcaccaa agacaccacc ctggaggggg ccgaatactt
    14341 cctggacatc cgcaatactg ggctcgtggg atacgccgag ctcactgtgc ccggcctcgt
    14401 cgaggacatg caggatgtct ttgagaaacg actctggctg cccagcggcg acggggatgt
    14461 gcaggggacg ctcgacgagg tcgccaagat ggcgaacgag gggatcaaga agcagtcgat
    14521 cgaataatgg tagtcgaggc ctcctggtgg gaggatggcc gtgacgccct ttccaccagg
    14581 ctatggccca tctcggaagg ggagcgcagg tgagtagctt ggtcaaggcg tcgaagctaa
    14641 gccagaggcg caagagcgac atcaggtggg gatacttttt tatcttcccg cagctcttcg
    14701 gggttctggc tttcgtcctc tttcccctgg tctacgtact cgtcctcagc ctgctcgaat
    14761 ggaacgggct gagcgaggcc acgttcgtcg gcctgtccaa ttactcaacc gagctgacca
    14821 gctctgaatt caggaacgcc ctgaccaaca ccgtctacta cacggtaatg gtggtgcctt
    14881 cgagtgtggt gctctccctg atggccgccc tggccctcaa caagctgcgc ctcaagaact
    14941 tcttccgtgt gatctacttt gcacctgtag tcacgagcgc cgtcgccgca ggcgtcgtct
    15001 ggttgtgggt ttacaaccct gacttcgggc tcatcaactc ctacctgcag aactggttcg
    15061 gcatcaaggg accgaattgg ctgctcgacc ccaagtgggt gatgccttcg atcgcgatca
    15121 tgagtatctg gctgagtatg ggattctaca tagtcatctt cctcgccggg ctgcagggca
    15181 tccctgcggc gtacctggag gccgcgcaga tagacggggc caacaggttc cagaggttct
    15241 ggtacgtgac gctcccgctg ctctcgccga ccacgttctt cgtgtttatc atcgccatca
    15301 tcaactcgtt ccaggtcttc gatcaggcct tcataatgac ggccgagggt ggtccggcta
    15361 actcggccgt taccatggtc tactacctga gggacctggc cttcgtagac ttcgtatacg
    15421 gcaggagtgc cgcggtagcc gtgatcctgt tcgcgatcat tctggccttc accctgctgc
    15481 agttcagggt gcagcggcag tgggtccact acgagtagag ctctaccggg aaaggaggca
    15541 gcatcgtggc aagcgtggcg acgacgaaag acgtatcagg cccggtagag tcgaccagtc
    15601 gcaccggccg tctcgtggcg tcagtcctgc tctacatagc cgtgtcctta ggggcggtcg
    15661 tcatgatcat gcccttcgcg tggatgttac tcgcgtccct caaggacctg gggcagatct
    15721 accaggtacc tcccaactgg atcccagacc ccttcgtgtt ccagaactac gtgaatgcgt
    15781 ggaccgcggt acccttcgcc acgggctaca tcaacagcgc catcgttacc gtgacggtcg
    15841 tcgtggcgaa ccttctcacg tgctcgatgg ccgcctacgc cttcgccaga atagagtttc
    15901 ctttcaggca cgcgctcttt atcctgtttc tggcgacgct tatggtcccc gagcaagtaa
    15961 cgataatccc tctctacata ataatagaga gccttggcct tatcgatacg cttctctccc
    16021 tgatcatccc ctacgccctg ttcaacgcct tcggcgtctt cctgctcagg cagttcatca
    16081 agagcctccc cgtagacctg gaggaggcgg ccatcgtgga cggggcgaac cggtggacga
    16141 tctactggcg gatcatcgtc ccgctcataa ggccggccct tgcggccttc ggcatcttca
    16201 cattcctgtt ccagtggaac aacttcttca ggccactggt tttcctgaac agcatcgaca
    16261 actatacggt gccccttgcc ataaactttt tccgcggcca gtacgcaaca gacttcgccc
    16321 tgctcatggc cggctcggcg atctccatcg tccccgtcct tctcgtctac gttataggac
    16381 agcgccagat catcgagggc atcgccacga cgggcttgaa gcaatagtgt caacgctgcc
    16441 ttttctggag atctagagac cgcttgccaa ccagcgacgg ctacatcgac acccatctca
    16501 gggagcctct ggtcgaggag aatgccttcg tgcggatgtc tcctgaaatc gcaccaccac
    16561 cctccttcga ggacgcgtgg gactccctgc cccggacctt ctgggaaggc cacgagggcg
    16621 tcatagattg ctaccgtagg gcgtgggaac tggcgttcgc caatgtgcgc cgtcccgagc
    16681 cagagagcgg cttcgtggcg agctttatcg acacggcttt caacgactgc ctgttcatgt
    16741 gggactcggc gttcatcttg cttttcgccc gctacggccg ccgcgccttc gacttccagc
    16801 gcaccctgga taacctctac gccagccaac accccgacgg atttatctgc cgggagatcg
    16861 ataccttcga cggcaaagac cgcttcgagc gcttcgacgc ggcgagcacc ggacccaacg
    16921 tcatgccgtg gacggagtgg gaacactact ccgacacggc cgacagcgaa cgccttggcc
    16981 tggtcttccc ggttctggtc tcctaccaca ggtggctcag gcgtcaccga acctggcgcg
    17041 acggaaccta ctggacgtcc ggcctcggct ccgggatgga taaccagccg cgcttcccac
    17101 agagaccgtc aggagatgcc gggtggccgg aacgcctcac ctaccacggg cacgccgtct
    17161 ggatcgacgc gtgcctccaa cagatcctct cggcagacct cctcttgcgg atggcagaag
    17221 ccctcggccg tctcgacgag gttgcagacc tgcgcgagga gcgtgaccgg ctctcgctga
    17281 cggtcaacga gcgtctgtgg aacgaagaga ctgccttctg ccacgacgag cttgcggacg
    17341 gaacgctctc ggacgtcaag accgtcggtg ccttctgggc cctgcttgca ggcgtggtgc
    17401 cgaaggagag gatctcttcg ttcgtcggac acctggaaga cccgaaaagg ttcgggcgac
    17461 cccatcgcgt accctcgctc tccgccgacc atccggaata cgacccggac ggcggctact
    17521 ggctcggcgg cgtatggccg cccaccaact acatggtcct gcgtggcctc gatcgagccg
    17581 gttacaacga cctggcgcac gagatctcgc gcgaagacct cgaccacgtg gtaggggtct
    17641 tcgaacagac cggaacgcta tgggagaact acgcccccga acggacggcg ccaggcaacc
    17701 gcgccaaaga cgatttcgtc ggctggagtg gcctcgcgcc cgtggcaggc ctcttcgagt
    17761 acgtgttcgg gctacgcgcc gacgcgccgt ccagacgact cctttgggac gtacggctcc
    17821 tggaaagaca cggcgtctac cgctacccgt tcggcaggga cggagttctg gatctctcct
    17881 gcgccacccg cagctccgct acagaggagc cgaagataca ggtgagctcc acagtctctt
    17941 tggagcttgt gatcaggtgg gagggtggag aaaggacagt cgcagtaccc gagggctgaa
    18001 gcccgaaacc tgataccctg aattatgaag caaatcgctt cgagacagga ggatcgaaac
    18061 gtggagacca ccaagcatct gacgcgagag cagatccagg gcttccagga agacggttat
    18121 ctcgtggtgg agagcctctt cgcgcccgag gaagtaaagg ctctggtgga caacttcatg
    18181 cagatgcatg cggaaggtcc tatcccgggc tgcttcgagc ctgcggatcc cgaagaagcg
    18241 gatggcgaca tactcaaact ctacccgcgc attatgcacc cgcacagggt caacgagctg
    18301 gcactgcggt acttgctcga cgacagactc gaggacattc tcgcggacct cttcggcgaa
    18361 gagccgctcg ccgcgcagag catgctgtac ttcaagccgg ccggggcgaa gggccaggcg
    18421 ctccaccagg acaacttcta cctcaaggtc gagcccggta cctgtatcgc cgcctgggtt
    18481 gctctcgatg ccgcggacca ggagaacggc gggctcgagg tcgtccctgg cacccacgat
    18541 atggagctgt tctgtcccga ggaggcggat accgacgtct ccttcacgcg cgactacgtg
    18601 cccgtgccag aaggtctcga agccgtgccc gtagtcatgc ggccgggaga cgtgctcttc
    18661 ttcaacggcc agctggtaca cggctcgcag ccgaatagta gcgaggaccg cttccgacgc
    18721 tcctttatct gccactacat aagccgtttt tccgagcgag tctcgaagtg gtaccgtccg
    18781 atcctgacga tggacggcga ggaggttcgg atcgaggaga gcgccggcgg agggccctgc
    18841 ggcaccgagg acgcgggcgt tcattgatag ccccaaacct tgaacgtggt ccctagtaca
    18901 tgtaacgtcc gtcagatacg gccgcagagg aaaaggatgc accagtgcca aagataaccc
    18961 tcatcggtgc ggggagcgcc gtcttcacgc gcaacctgtg ctcggacatt ctgctcgctc
    19021 cggcgttaca ggagagcatg atctccctta tggacataga ctcggacagg ctgcgcacct
    19081 cccgcgacct cgtccagaac atagtagacc gccgcgggct cgacgccagg gtggaggcca
    19141 ccaccgaccg tcgggaggcg atagagggcg ccgactatgt ggtgaccaca ttccagcaag
    19201 gcgggctcga cgcatacgcc ctcgatatcg agattccaca gcgctacggc gtcgagcagt
    19261 gtgttggcga cacccttggt cctggcggcg tcttccgtgc cttgaggacc atccctgtct
    19321 tgcttgacct gtgcgacgag atggacgaac tggcccctaa cgcccagctg ctgaactacg
    19381 tcaacccgat ggcggccaac tgttgggcgg tggatgtggg atcggggaga cctcacgttg
    19441 gcctatgcca tagcgtccag gggactggcg agatgctcgc ttcgtggatc ggggtgccct
    19501 acgaggaggt caacttcctg tgcgcaggta tcaaccacca ggcgtttttc ctggagttcc
    19561 gcagcggcag ggatgacctg tacccggaga tcagggacgc catcagacgg gatgacatac
    19621 gtggacagga acccgtgcgc gtcgagatca tggagcattt cgggtacttc gtgacagagt
    19681 cgagcggcca cgccagcgaa tacgcaccgt actttcgcaa ggacgcgcgg atggtcgaaa
    19741 aggagctcgt gccgcgcttt gtggacgaag ccgaccactg gttcgatttc ggccgcaccg
    19801 gaggatacct gcgccactgc ctgagccgtc tggaggagcc gccggacggc cacggacctc
    19861 tccccacaga tcgcacccac gagtacggct cgtacatcat cgaagccatg gagacagacg
    19921 agccgatcaa gatcaacggc aacgtgccca accgcgggct catggatagt ctgccgaatc
    19981 gttgctgcgt tgaggttcct tgcctggtcg acgccaacgg cgtccagccc acggtgatcg
    20041 gacactatcc gccgcagctc gcagccctca accggaccaa catcaacgtg caggagttga
    20101 ccgtcgaggc cgccctgtcg ggcgacgtgg aggcggtaca tcacgcggtt atgctcgacc
    20161 ccctgacggc ggccgtctgc actctgcccc agatccgctc gatggtagat gagatgctcg
    20221 aggcgcaggc gaagtggctg ccacagttca ctgagatcac agcctagccc gccatggacc
    20281 gcacacgagt aaaggccggc ggcatcgagg tgaggctgga cgggggagag aagggcttcc
    20341 ggagctccct ctcgacctcg gcgctggaga gtggtgtcga gctggttcat ctgcggatag
    20401 aatccggcga cgtagcacgc ccgccggtgt tccgcctctc gtggttctac ccgcttgtga
    20461 gcgtgcacgg cttctggcac tccggcgcgg gatacgacaa gggcttgggg gtggattggg
    20521 gaaagggctt ctattccaaa gcgacctctg gcgcgcctgt cggctgtctg tacgatctca
    20581 agggtcgtaa ccgcctgact ttcgcactct ccgacacgct cgaccctgtt acattccacg
    20641 ccggagtcca cgaagagagc ggcgaggcga ggtgttgggt caggttcttc gatgaaccgc
    20701 cggcgccgct gagtacctac gagactacgc tcaggctcga cactagggac ctcccttacc
    20761 accaggttct gtcagacgtg agccggtggt gggcaggtct gcctggatac gagccggctg
    20821 gggtaccgga aacggcgcgg ttgccgatgt actccacgtg gtacagcttc caccaggacc
    20881 tcgacccagg gcgtgtcgag gagcagtgtc gcctggcgag agaactggga tgcgaggcgg
    20941 tgatcgtaga cgacggctgg cagaccacga gcaacgagcg gggatatgcg tacacgggcg
    21001 actgggagcc ggcgcccgag aaagtccctg acatgcgtgc gcacgtagac agggtgcacg
    21061 ccctcgggat gaaggtcctc ctctggtact ccgtaccgtt cgtcggccgc cacagcaagg
    21121 tgtgggagcg gttttcgggg atgttgctag aggagatcga acgccacggg gcgggtgtcc
    21181 tcgacccgcg cttccccgag gtgcgcgagt tcctgatcgg gacctacgag tcggccctga
    21241 gagagtggga cctcgacggc ctcaagctcg attttgtaga tagcttcggc gcttctaaag
    21301 aggccggcgg cggccgcgat tacgacggcg tgcccgaggc ggttgacagg cttctggagg
    21361 atacgatagt gcgcctccgc cggataaaac ccgacgttat ggtggagttc cgccagagtt
    21421 acgtcggtcc cttgatgcgc aagtacggca atatgttccg ggcgatggac tgcccgaacg
    21481 acgccgtcga gaacaggatg cacaccctgg acatcaggct gctctgcggg gataccgccg
    21541 cgcactccga catgctcatg tggcataccg aggagcccgt acaaagcgcg gcgcttcagc
    21601 tggtgaacgt actcttcagc gtgccccaga tctcggttct tctggaccgg attccgcccg
    21661 aacacctgcg gatgttgcgc ttctggctgg gcttctggcg tgaacatcgc gacgtacttc
    21721 tggatggcgc cctggaaccg ctccatcctg aggccatcta ccccgtcgtg ctcgcacgca
    21781 cggagtccaa gctggccgct gtggcctacg ggaacgccgt cgttccgctc gaaggggaga
    21841 ttccctcgac gctctttgtc gtaaacggca ccctcgagga gggggtcgtg ctctctctcg
    21901 cggacgacgc aggtacacgc gggatagaag tgcgggattg caggggacag gttacgcgta
    21961 cggacgactt cgggctcgag accgggttgt gccggatcga catcc
//
LOCUS       DATMVF010000190         9217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_248386, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000190 DATMVF010000000
VERSION     DATMVF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248386"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VN178_09405"
     CDS             complement(<1..545)
                     /locus_tag="VN178_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWS81228.1"
                     /translation="MKRVVSARADIVGSLLRPPELLEARERYAAGDLSAAEFKRIEDR
                     AVDWAIGLQERTGLSVVTDGEMRRESFQSQLVEATGGFGEHDIDAWLWGEWHGDRAVG
                     DKTTERPQTLGVVGKLQRRRHLSVEEFVYARGRTERTVKVTLPSPSMYANLWSPELSA
                     GAYPTLDLFLEDVARILREEV"
     gene            complement(583..786)
                     /locus_tag="VN178_09410"
     CDS             complement(583..786)
                     /locus_tag="VN178_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81229.1"
                     /translation="MIRVCIEVDRDTANPTTLFVRAASIVRALEVAGEQNPGCALNVV
                     FPLDPDTFFVPGVSEGVEAEIAA"
     gene            1017..2183
                     /locus_tag="VN178_09415"
     CDS             1017..2183
                     /locus_tag="VN178_09415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWS81230.1"
                     /translation="MKLARRMERLGTETAFSVLAKAKALEAEGRGIIHLEIGEPDFDT
                     PDHVVEAGCKALRDGHTHYTPTAGIPELREAIAVDVARSRSIDVGPEQVVVTPGGKPI
                     MFFTILALVEEGDEVLLPNPAFPIYESMVNFVGGRHVFVPLRQENGFRFDLDEFRAGL
                     SERTKLVILNSPANPTGGVLTAEDIAGLAAILRERPDIYVLSDEIYSRLLYSGDFASI
                     ASEPGLDTDGRTIILDGFSKTYAMTGWRLGYGVMPAPLAEEVAKLQVNSNSCTNAATQ
                     YAGLAALQGPQDAVGSMLAEFRARRDLIVDGLNELPGVECITPQGAFYAFPRIAETGL
                     SADKLADLLLAEAGVACLSGTAFGRHGEGHLRLSYANSRENISKALQSMGEVLW"
     gene            2365..4356
                     /locus_tag="VN178_09420"
     CDS             2365..4356
                     /locus_tag="VN178_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor GTPase family protein"
                     /protein_id="HWS81231.1"
                     /translation="MKHLNLGIVAHVDAGKTTLTERLLFAAGVIDQIGRVDDGSTQTD
                     SLALEKQRGITIKAAVVSFVVDDVTINLIDTPGHPDFIAEVERVLGVLDGTVLVVSAV
                     EGVQSQTRLLMRTLQRLRVPTLIFVNKIDRKGAQCERLLHSIADRLTPAIIPMGSTSG
                     LGTRNPDFAPYDAADADFTSELVDLLADHDDALLAAYVDDERAVTYRRLRGELAGQTG
                     KGLVHPVFFGSAITGAGVEELVDGITELLPTSEGDVDGPLSGTVFKVERAPAGEKIAY
                     VRMFSGTVRVRDMLRFGREEDKVTAISVFERGSAVQRESVGAGQIGKLRGLGEVRIGD
                     AIGEDRTTLDSHYFAPPTLETVIVPSRPAEKGALHVALTQLAEQDPLINLRQDDIRQE
                     LHVSLYGEVQKEVIGATLENEYDIDVEFRETTAICVERPIGVGEAVELIPKARTPTTP
                     FLATIGLRIEPAAPDSGVQFGLDVQVGSIPTHVFKTVEAFHEAMERNVLETLSQGIYG
                     WEVTDCCVTMIDCDYQAPPRRWPGTTASDFRLLTPLALMGALKQAGTVVCEPIHRFHL
                     EIPSDMFGTTVSALARLSAVPQTQEMRGSSYILEGEVPANRVHTLQQHLPALTRGEGL
                     VECEFDSYKAVCGKVPTRPRTDYNPLNRKEYLLHLMRRV"
     gene            4374..5102
                     /locus_tag="VN178_09425"
     CDS             4374..5102
                     /locus_tag="VN178_09425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS81232.1"
                     /translation="MKDYEPIMSFGEDVAKTYDDVQRGDEAEAVAFLEKLAGGGPALE
                     LAVGTGRIALPLAGRGIRVDGVDISPDMVARLRAKPGGDRISVTIGDFADVPVPGTYR
                     LIYVVFNTLFNLLTQEDQVRCFQNVAAHLTGDGSFVIEAGSPAFLYGLRNDQHVDAES
                     IEIDAVRLDVLRHDPAKQMLDESHVSISNAGVRLDPIVQRYAWPAELDLMARIAGLRL
                     KERWDGWNREPFTNRHNVVSVYER"
     gene            5265..6050
                     /locus_tag="VN178_09430"
     CDS             5265..6050
                     /locus_tag="VN178_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HWS81233.1"
                     /translation="MSRGRRTQGEPTAETQAASRAERVARRLRLPLIVAALLAIPTII
                     VQESDLGGFWGILAAVLDWCIWAMFAANLAIMLSIVPNRRRWLTENPLDVLIVVFTPP
                     FLPATMKLARVLPIVRLIWLMAIAHRLRDVFSLQGLRYAALIVFTVVVGGGVIFVAVE
                     PGQDLSTWDGLWWATETVTTVAYGDIYPTTALGRIVATIVMTAGIGFVALLTGALAQR
                     FLYGGSAGTTPEPDPDRAEIVQKLDELSNQIAELQQALKERHE"
     gene            complement(6207..>9217)
                     /locus_tag="VN178_09435"
     CDS             complement(6207..>9217)
                     /locus_tag="VN178_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWS81234.1"
                     /translation="LLWSDLEPEAALNNLHHTLHVARRVLEPSAPASATSRYLRLWDQ
                     RLALCPEGPLWVDVEAFEEAATAARHTLEPAAFRVAIDLYSGELLPQDRYEVWAEERR
                     AQLRGLYLSLLMEVAGLYEERKEFGEAIEALGKVIAEDRTHEGAHVGLMRLYALLGRR
                     REALRQYERLRDALTRDLGSQPEGATVHLHKEIWAGTFPPPDSSLPAGSSPEDLRAAE
                     AGGRHNLPLARTSFIGRERETLEVKRLLSMTRLLTLTGVGGSGKTRLALEVAKDLVGA
                     YPDGAWLVELASLSDPALVSQAVARALKVREQPGRPLLETLEDTLRTKKMLLVMDNCE
                     HLIEAVVRLIDVLLDSCPGLRVLATSRETLSAAGEVAWVVPSLTVPDYGQASMAENVE
                     GYESVRLFVERAGQRDPSFVLTPRNATAVAQVCQRLEGIPLAIELAAARIGVLPVERI
                     AERLDDSLRLLTAGGRTALPRQRTLRATLEWSHELLGEPERVLFRRLSIFAGGLTLEA
                     AEVVGSGGGIEQHDVLDLLSKLVDKSLVMSEVPRYRLLEPLRQYCQERLEGSGEAQWV
                     RERHAEYYLALAEGADAQDAERELNAARPVAWLKRMESEHANLRTALDWSLDEPDGRD
                     TAELGLRLAVALWWFWHTRDYLTEGRRYLEKAGSGMSNPTTTRLRARALDGAAWLALY
                     QGDQVASKALIEEALVLYRQLQDEEGIASGLTDLGLVAVLGQWDDISLPAVLEELGEL
                     KPRLKNRNTLAYLLMLEGMIAMSRGDLEHSVTLHEQSLELFREIQNTQGTITCLGHLG
                     LLALILGDCESAPAMLRESLHLAWELDYKQSIQHCLHTLACVMARREQPVRAARLWGV
                     VQGMEEAYGAHLTPIILSLTDYEGHLSRGRSQLGEEEFAAAWAEGKAMSPGQAIEYAR
                     SDEEVRELPTLVSQQQPLPEERTEKLTRREREVALLVAQGLTNPQIASELSVSRSTAN
                     NHVARILRKLGLRSRAQIAAWVTERRPPSS"
BASE COUNT         1699 a         3033 c         2798 g         1687 t
ORIGIN      
        1 tcgacctcct cgcgcaggat ccgcgccacg tcctccagaa agagatcgag cgtcgggtag
       61 gcccctgcgg atagctctgg cgaccagagg ttcgcgtaca tgctcgggct cggcaacgtg
      121 accttcacgg tcctctccgt ccgaccccgc gcatagacga actcctcgac cgagaggtgg
      181 cgcctccgct gcagctttcc gacaacgccg agggtctgtg ggcgttcggt cgtcttgtcc
      241 cctaccgccc tgtctccgtg ccactcgccc cacagccagg cgtctatgtc gtgttcaccg
      301 aagcctcctg tggcctctac cagctggctc tggaacgatt cccggcgcat ctcaccgtcc
      361 gtgacgactg agagacccgt ccgctcctgc aaccctatag cccagtctac ggccctatcc
      421 tcgatgcgtt tgaactcggc tgcggagagg tcacctgcgg cgtatcgttc gcgtgcctcc
      481 agcagttccg gaggtctcag gagactgccg acgatgtctg cccgggcact aaccactcgc
      541 ttcacacgag ccagtctagc acccgtccgc gaccggggaa gcctaggctg ctatctcggc
      601 ctcgacccct tctgagacac ccggtacaaa gaaggtatcg gggtcgagag gaaacaccac
      661 gttcaaagcg caaccggggt tctgttcccc tgccacctct agcgccctta cgatgctggc
      721 ggccctcacg aatagtgtcg tggggttcgc cgtgtcgcga tccacctcaa tacaaactct
      781 aatcattcgg gtcctccatc tgttaaccag tcagcgattc cctctatttc ccttctacac
      841 cttttctgtt acatatcggt aacagcggcg caaaatgtag gcaacaagcg gtacgtgcct
      901 ggagcggccc tggatcatac gcttcgccgt atccgacact tcttgctccg tgctgcagta
      961 ctttggtgtg gtgtaatgcg gtaacgtacc tgggtctcga tcgagcggag gacggcgtga
     1021 aactggcgag gcggatggag cgtctgggaa ccgagacggc tttttcggtt ctggcgaagg
     1081 cgaaggctct ggaggcggag gggcgtggga tcatccacct cgagatcggc gagcctgact
     1141 tcgacacgcc ggatcatgtc gtagaggcgg gctgcaaagc cctgcgcgac ggacataccc
     1201 actacacccc caccgccggc attccggagt tgcgcgaagc tatcgccgtc gatgtcgcgc
     1261 gtagcaggag tatagacgtc ggtcccgaac aggtggtcgt cacgcccggt gggaagccga
     1321 taatgttctt caccatcctg gcactcgtcg aggagggtga cgaggtgctg ctgcccaacc
     1381 cggcgttccc gatctacgag tcgatggtga acttcgtcgg cggaaggcac gtcttcgtac
     1441 cgctgcgcca ggagaacggg ttccgtttcg acctggacga gttcagagcg ggcctcagcg
     1501 aacgaaccaa acttgtgata ctcaactccc cggccaaccc gacgggcggc gttctcaccg
     1561 ccgaggacat cgccgggcta gccgccatcc tgcgcgaacg cccggacatc tacgtcctga
     1621 gcgatgagat ctactcccgg ctgctctact caggggactt cgccagcatc gcctcggagc
     1681 ccggactgga caccgacggg cggacgatca tactcgacgg cttctcgaag acttacgcca
     1741 tgacgggctg gcggctcggc tacggcgtaa tgcccgcacc cctcgcagag gaagtcgcca
     1801 aattacaggt caactccaac tcgtgtacca acgcggccac gcagtacgcc ggtctcgcag
     1861 ccctccaggg tccccaggat gcagtgggca gtatgctcgc cgagttcagg gcgcgccggg
     1921 acctgatcgt agacgggctc aatgagctcc cgggcgtcga atgtataact ccccaggggg
     1981 ctttctacgc cttcccacgc atcgccgaga ccggactttc ggcagacaag cttgccgatc
     2041 ttctcctcgc cgaggcaggc gtagcgtgcc tctccggtac ggcgttcggg cgccacggcg
     2101 agggccatct gcgcctctcc tacgccaact cccgcgagaa catcagcaaa gcgctccaga
     2161 gtatgggcga ggtcctctgg tgataatcgc aggatcttcg acgcctggaa aagccggctg
     2221 aatcgcccgg cgaatgggtt atcttcagtg acggccttct gctacacttc gaacgacggg
     2281 tgatcgaaga aggagaggaa atgcgccagg ctttcatgtc tatccagagg cgaataaaca
     2341 cctactcgaa ggacatgaag ctccgtgaaa catttgaacc ttgggatagt ggcgcatgta
     2401 gacgccggta agaccaccct gaccgagcgg ctgctcttcg ccgccggggt catcgaccag
     2461 atcggcaggg tcgatgacgg aagcacccaa accgattcac tagcactcga aaaacagcgc
     2521 ggcatcacga tcaaggctgc cgtcgtctcc ttcgttgtag acgacgtcac tatcaacctg
     2581 atcgacaccc cgggccaccc ggatttcatc gccgaggtgg agagggtgct cggcgtgctc
     2641 gacggcaccg tgctcgtcgt ctccgcggtg gagggtgtgc agtcgcagac ccgactattg
     2701 atgcggacgc tacagcggct gcgcgttcct accctaatat tcgtgaacaa gatcgaccgc
     2761 aaaggcgcgc aatgcgaacg cctactgcac agcatcgccg acagactaac gccggcgatc
     2821 atcccgatgg gatcgaccag cgggttggga acccgcaatc ccgatttcgc gccttacgac
     2881 gccgctgacg ccgacttcac ttccgagctg gtcgatctgc tcgccgacca cgacgacgcg
     2941 ttgctcgccg cctacgtcga cgacgagaga gcggtcacct accgacgact ccgtggcgag
     3001 cttgcggggc agaccgggaa gggactggtg catcccgtgt tcttcggctc ggcgatcacg
     3061 ggcgctggcg tggaggagct ggtcgatggg atcacggagc tcctgcccac ctcagaaggc
     3121 gatgtcgatg gaccactctc agggaccgtc ttcaaggtcg aacgggctcc ggctggagag
     3181 aagatcgctt acgtccgcat gttctccgga acggtacggg tgcgagatat gctgcgcttc
     3241 ggcagggagg aggacaaagt cacggcgatc agcgtcttcg agcgcggctc ggccgtccag
     3301 cgcgagtcgg tcggcgcggg gcagatcggc aagctcaggg gtctcgggga agttcgcata
     3361 ggggacgcga tcggtgaaga tcggacgacc ctggatagcc actacttcgc accgccgacc
     3421 ctcgaaacgg tcatcgttcc cagccgcccc gcagagaaag gcgcgctgca cgtggcgcta
     3481 acccaactcg ccgagcagga cccgttgatc aacctgcgac aggacgatat acgccaggaa
     3541 ctacacgtct cgctctacgg cgaggtgcag aaggaggtca tcggggcgac gctcgagaac
     3601 gagtacgaca tcgacgtcga attccgcgaa acgacggcga tctgcgtcga acggcccatc
     3661 ggcgtcggcg aggccgtcga gttgatcccc aaggcacgta ctccgaccac tccgttcctc
     3721 gccaccatcg ggctgcgcat cgaacctgcc gcgcccgact cgggggtgca gttcgggctg
     3781 gatgtccagg tggggtctat accaacccac gtcttcaaga cggtcgaggc ctttcatgag
     3841 gcaatggagc gcaatgtact agagacccta agccagggga tctacggatg ggaggtcact
     3901 gactgctgcg tcacgatgat cgactgcgac taccaggccc caccacgacg gtggcccggg
     3961 accaccgcct cagacttccg tctgctgacc ccgcttgcgc tgatgggcgc gctcaagcag
     4021 gccggcaccg tggtgtgcga gccgattcac cgtttccacc tcgagatccc gtcggatatg
     4081 ttcggaacga cggtgtccgc attggcgcga ctgagcgccg ttccacaaac ccaggagatg
     4141 cggggctcat cgtacattct ggagggtgag gttccggcga accgggtgca caccttacag
     4201 caacatctac cggcgctaac ccgcggcgaa ggcctggtgg aatgcgaatt cgactcctac
     4261 aaagcggtct gcggcaaggt cccgacccgg ccgcggacgg actacaaccc gctcaaccgc
     4321 aaagagtacc tgctgcacct catgcggcgg gtctagagca aagggaggtt tccatgaagg
     4381 actacgagcc gatcatgagt tttggcgagg atgttgccaa aacgtacgac gacgtgcaac
     4441 gcggggacga ggccgaggcc gtcgcgttcc tggagaagct ggccggcggt ggtcccgcgc
     4501 tggagctcgc cgtcggcacc ggcaggatcg ccctaccgct cgccggtcgg gggatccgcg
     4561 tggacggagt cgacatctca cctgatatgg tcgcgcggct gcgcgccaag ccaggaggcg
     4621 accggatctc ggttacgatc ggcgacttcg ccgacgtgcc ggttcctggc acgtaccgcc
     4681 tgatctatgt cgtcttcaac acactcttca acctgctgac ccaagaagac caggtgcgct
     4741 gcttccagaa cgtcgccgcc cacctcaccg gcgacggctc gttcgtgatc gaggccggct
     4801 cgcccgcctt tctctacggg ttgcgtaacg accaacacgt cgatgcggag tccatcgaga
     4861 tcgacgcggt acggctcgat gtgcttcgtc acgatccggc gaagcagatg ctcgacgaga
     4921 gccacgtgtc catctctaac gcgggcgtgc ggctggaccc gatcgtccag cgatacgcct
     4981 ggccggccga gctagacctc atggcgagga tcgccgggtt gcgcctcaaa gagcgctggg
     5041 acggctggaa ccgcgagccg ttcaccaaca ggcacaacgt cgtgtcggtc tacgaacgct
     5101 gagatgattt gtcgtgctgt cgaagcgtaa cgtcgaggta gtcctcgccg atcttgcgca
     5161 agacctccta cagcatcctt cacagcctcg cccccgttcg gtatcttggt gagatttatt
     5221 ggagatcggg tttcggcggg cgtataaggg gaggcactca gggtatgtcg agaggacgca
     5281 ggacgcaggg tgagcccacg gcggaaacac aagccgcttc gcgtgccgag cgcgtggcaa
     5341 ggcggctacg tttgcccctg atcgtcgccg cgctgctggc cattccgacc atcatcgtac
     5401 aggagtctga cctgggcgga ttctggggga ttctcgccgc ggtcctggac tggtgcatct
     5461 gggcgatgtt cgcggccaac ctggcgatca tgctctccat agttccgaac cggcggcgct
     5521 ggttgaccga gaatcctctg gacgtgctca tagtcgtctt taccccgccg ttcctcccgg
     5581 ccacgatgaa gctggcgcgt gtgttgccga tcgtaaggct gatctggctt atggcgatcg
     5641 cccatcgcct ccgagacgtc ttctcactac agggattgcg ctacgcggcg ctgatagtct
     5701 tcaccgtcgt ggtcggcggg ggtgtgatct tcgtcgccgt cgagcccgga caggacctga
     5761 gcacctggga cgggctctgg tgggccacgg agaccgtcac gaccgtggct tacggggaca
     5821 tctaccccac aactgccctg ggtcgcatag tcgcaaccat agtaatgacc gccggcatcg
     5881 gcttcgtcgc cctgctgacc ggcgcgctgg cccaacgctt tctttacggc gggagcgcag
     5941 gcacaacccc ggaacccgat ccagaccggg ccgaaattgt gcagaagctc gacgagttgt
     6001 ccaaccagat agcagagctc cagcaagccc tgaaggaaag gcacgagtga cccaggccgt
     6061 tagcctttga cagtccctag tccggccact ctccctccgg agccgtcgct ggagggcgta
     6121 gttactgttg actatttttg ggccaagccg agtaagagat gactcgccac cataacgtcc
     6181 ctcgggcggg aagcctgagg acaccgttac gaggaagggg gacgccgttc ggtcacccag
     6241 gcggcgatct gggcgcggga tctgagcccc agcttgcgca ggattctggc cacgtggttg
     6301 ttggcggtgc tcctggagac cgagagctcc gaggcaatct gggggttggt gagcccctgc
     6361 gcgacgagga gcgcgacttc tcgctcccga cgggtgagct tttccgttcg ttcctcgggc
     6421 agtggctgct gttgggagac aagcgtgggg agctcccgca cctcttcatc cgagcgggcg
     6481 tactctatgg cctgcccggg cgacatcgcc ttgccctccg cccacgccgc cgcaaactct
     6541 tcctctccca gttgcgagcg ccctctgctc aggtggccct cgtagtcggt gagagagagg
     6601 attatggggg tgaggtgtgc gccgtaagcc tcctccatgc cttgcacaac cccccacaac
     6661 ctggcggccc ggactggctg ctcccggcga gccatcacgc acgccagcgt gtggaggcag
     6721 tgttggatgc tctgtttgta atccaactcc cacgccaggt gcaggctttc ccgcagcatt
     6781 gcaggagcac tctcgcagtc gcccaggatc aatgccagga gcccaaggtg gcccaggcac
     6841 gtgatcgtgc cctgcgtatt ctgtatctcg cgaaacagct cgaggctctg ctcgtgcagc
     6901 gtcaccgaat gctccaggtc gccccggctc atcgctatca tgccctcgag catgagcaag
     6961 taggcgagcg tgttgcggtt ctttaggcgc ggcttgagct ccccgagctc ctcgagcacc
     7021 gccggcaagg atatgtcgtc ccactgtccc agcaccgcga ccaaaccgag gtcggtgagt
     7081 ccggatgcga tgccctcctc gtcctgaagc tgccggtaga ggaccagcgc ctcctctatg
     7141 agagccttcg acgccacctg atccccctga tagagcgcca accaggcggc gccatccaac
     7201 gcccgcgccc tcaagcgagt cgtggttggg ttgctcatcc cggagccggc cttctccagg
     7261 taccttcgcc cctcggtcag gtagtctcgc gtgtgccaga accaccagag cgcgaccgcc
     7321 agccgcaacc ccagctccgc agtatctctg ccgtcgggct cgtcgagcga ccaatcgaga
     7381 gccgtcctga ggttggcgtg ttcgctctcc atccgcttga gccacgccac cggccgcgct
     7441 gcgttcaatt cgcgctctgc atcttgcgca tccgcccctt ccgcaagcgc caggtagtat
     7501 tctgcgtggc gctccctgac ccactgcgcc tccccactcc cctccagtcg ctcctggcaa
     7561 tactgcctga ggggctccag tagcctgtac cgcggaacct cggacatcac cagcgacttg
     7621 tctaccagct tggataatag gtccaggacg tcatgttgct cgatgccgcc gccactaccc
     7681 accacctcgg cggcctccaa cgtcaaaccc cccgcgaaaa tagaaagccg tctgaagagc
     7741 acccgctcgg gttcgcctag caactcgtgg ctccactcaa gcgttgccct gagcgtcctt
     7801 tggcgtggca gtgccgtccg cccgcctgcg gtaaggagtc tcaaggagtc gtcgagcctt
     7861 tcggcgatcc gctccacagg gagcacacct attcgcgcag ccgccagctc tatggccaaa
     7921 gggatgccct ccaaccgctg gcatacctgc gccaccgccg ttgcgttgcg gggcgtcaat
     7981 acgaaggagg gatcgcgctg accggctcgt tctacgaaca gcctgaccga ctcgtaacct
     8041 tcaacgttct cagccatcga ggcctgcccg tagtcgggca ccgttaggga aggcaccacc
     8101 caggcaacct cgccagcggc gctgagcgtc tcacgactgg tcgcgagaac ccgcaggccc
     8161 gggcaggaat cgaggaggac atcgatcagg cggaccaccg cctctatgag gtgctcgcaa
     8221 ttgtccataa ccaacagcat cttcttcgtg cgcaaggtgt cctcgagcgt ctcgaggagc
     8281 gggcgtccgg gctgctcgcg taccttgaga gccctggcca ccgcctgcga caccaacgcc
     8341 gggtcagaga gcgatgccaa ctctaccagc catgctccat ccgggtacgc tccgacgagg
     8401 tcctttgcca cctcgagcgc cagtcgcgtc ttgcccgagc cgccaacccc cgtgagcgtc
     8461 aacagccggg tcatggaaag cagccgcttg acttccagtg tctcgcgctc gcgtcctatg
     8521 aagctggtgc gcgccagggg caggttgtgc ctgccacctg cctccgctgc acggagatct
     8581 tccggtgagg aaccagcggg cagtgacgaa tcgggtggtg ggaaggtgcc ggcccagatc
     8641 tccttgtgta ggtgtacggt cgcaccctcg ggctgcgagc caaggtccct agtgagggca
     8701 tccctgagcc gctcgtactg cctcaaagcc tccctccgcc tccccaggag ggcatagagt
     8761 cgcatgagcc ccacgtgcgc tccttcgtgc gtacgatctt cggcgatcac cttccccaac
     8821 gcttcgatag cctccccgaa ctccttacgc tcctcgtaga gtccggccac ctccatgagc
     8881 agtgagaggt acaagccccg tagctgtgcc cgcctctcct ccgcccagac ctcgtagcgg
     8941 tcctgcggca gaagctcccc cgagtaaaga tctatggcca cccgaaaggc cgcaggctcc
     9001 agggtgtggc gggccgcggt cgccgcttcc tcgaaggctt cgacgtccac ccacagcggc
     9061 ccttcgggac acaaggcgag ccgctggtcc caaagacgca agtagcgcga agtagcacta
     9121 gccggagccg agggttcgag gacgcggcga gctacgtgca aggtgtggtg taggttgttg
     9181 agggcagcct ctggctctag atccgaccac agaagct
//
LOCUS       DATMVF010000191         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_168168, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000191 DATMVF010000000
VERSION     DATMVF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168168"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VN178_09440"
     CDS             complement(<1..780)
                     /locus_tag="VN178_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWS81235.1"
                     /translation="MGAKVMSTSSPEQRSVFRKKPVEAFVTETHPDEEGGELSRSIGL
                     FQLTMFGVGATIGTGIFIILTEAVPEAGPAVIVSFILAGITAALTAVCYAELASTIPV
                     SGSSYSYTYATMGEIVAFGVAACLLLEYGVSSAAVSVGWAQYLDELFSNLFGFRMPEA
                     ILAAPGEGGLLNFPAIVLVTLCCLLLMRGARESATVNAVMVVIKVAVLILFVIIAFTA
                     FSGENFTPFAPLGFAGIGVAASSIFFTFIGLDAVSTAGEEVH"
     gene            complement(943..2241)
                     /locus_tag="VN178_09445"
     CDS             complement(943..2241)
                     /locus_tag="VN178_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWS81236.1"
                     /translation="MCSVRAGERLRPEDLGFGRLFERIRDAVIVADAKTQRIVLWNAA
                     AAKMFGYPASQALTMRVEELVPEPLKDAHRAGITRYARTGHGRYIDSNAPLDLPAIKK
                     NGQEIRVELSLSPIRPVHDTDDGEAQFVLAIIRDVTERKRAEEAVRRLNKDLENRVEE
                     RTSQLEASIAELESNQQELRHSEEIFRLLVEGVRDYAIFMLGPEGHVASWNEGAERIQ
                     GYEVSEVVGEHFSVFYTEEDIDRGLPGEELRIAAIEGRFEEEGLRVRKDGTPFQADVV
                     ITALRDDSGELRGFSQVTRDITARKEAEEALRGSLRKMADLKAALDESAVVAITDANG
                     KITYVNDKFCEISKYYMEELLGQDHRILNSGHHTREFFEDLWSTIATGEIWRGEIKNQ
                     AQDGSLYWVQTTIVPFLDEAGRPDRYFAICNDITRYKEAE"
     gene            2506..>3010
                     /locus_tag="VN178_09450"
     CDS             2506..>3010
                     /locus_tag="VN178_09450"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HWS81237.1"
                     /translation="MAGTVERRRQHESSDERQAEIVDVLVDAFSGLMDANPEAFRQKF
                     RKMAADPFAFYRGSACLFYADMEREEDRWADERTSRVWIQGDLHAENFGTYMDGDGVF
                     IFDVNDFDEAYLGYFTWDIKRMVASVALLAWMKAISDADIASLIETYVRAYVEQVRYF
                     VESDRDHE"
BASE COUNT          548 a          956 c          927 g          579 t
ORIGIN      
        1 gtgaacctcc tcgcccgccg tcgagaccgc atcgaggccg ataaaggtga agaagatcga
       61 ggacgcggcc acgccgatgc cggcgaaccc gagcggggcg aacggcgtga agttctcacc
      121 cgagaaggcc gtgaacgcga ttatgacgaa aagtatcagc acggcgacct tgatgaccac
      181 catgacggca ttcaccgtcg cggattcccg agccccgcgc atgagcagca aacagcacag
      241 cgtcacgagc acgatggcag ggaagttcaa gagcccgcct tctcccggcg ccgcgagtat
      301 ggcctccggc atgcggaacc cgaacaggtt ggagaaaagc tcgtcgaggt actgcgccca
      361 cccgacggag acggcggcgg aagagacgcc gtattccagg agcaggcacg cggcgacacc
      421 gaacgccacg atctccccca tagtggcgta ggtgtaggag tacgacgagc cggagacggg
      481 gatggtcgag gcgagttccg cgtagcacac agcggtcagg gcggccgtaa tcccggcgag
      541 gatgaacgac acgatgaccg cgggtccggc ctcgggcacc gcctcggtca gtatgatgaa
      601 gatcccggtg cctatcgtgg ccccgacccc gaacatggtg agctggaaca ggccgatcga
      661 acggctgagc tcgccgcctt cctcgtcggg gtgcgtctcg gtgacgaacg cctcgacggg
      721 cttcttgcgg aacacgctcc gctgctcggg cgatgatgtg ctcatgactt ttgcgcccac
      781 ggtgagcgtc cgggcgctcc tcgcaagcgt tcaccgacaa aactcgctcg tccaggcggg
      841 tttgccaccg ttcttcctcc tagttcccag cttgagttag caatctcctg gtaacaatgg
      901 tagttttctg gcaggccccg gtcaagaggt cgagccgggc gcctattcgg cctccttgta
      961 cctggtgatg tcgttgcaga ttgcgaagta tcgatccggc ctgccagcct cgtccaggaa
     1021 aggcacgatg gtggtctgta cccagtagag actaccgtcc tgtgcctggt tcttgatctc
     1081 gccccgccag atctcgcccg tggcgatggt actccacaga tcctcgaaga actccctggt
     1141 gtggtggccg gagttgagaa tgcggtgatc ctggcccaag agctcctcca tgtagtattt
     1201 ggagatctcg cagaacttgt cgttgacgta cgtgatcttg ccgttcgcgt cggtgatcgc
     1261 gacgaccgcg gattcgtcca gggccgcctt caagtccgcc attttcctga gcgagccgcg
     1321 caacgcctcc tcggcctcct tgcgggccgt gatatcgcgc gtcacctgag agaagccccg
     1381 gagctcaccc gagtcgtccc tcagggccgt gataacgacg tcggcctgga agggcgtgcc
     1441 gtccttgcga acccgaaggc cctcttcttc gaagcggccc tcgatggccg cgatgcgtag
     1501 ctcctccccg gggaggccac gatctatgtc ttcctcggtg tagaagaccg agaagtgctc
     1561 gccgacgacc tccgagacct cgtagccctg tatgcgctcg gcgccttcgt tccaactcgc
     1621 gacgtgtccc tccggaccga gcatgaagat cgcgtagtcc ctgactccct cgacgaggag
     1681 ccggaagatc tcctcgctgt gacgcagctc ctgctgattg ctctccagct ctgcaatgga
     1741 ggcttccagc tgcgaggtgc gctcctcgac ccggttctcg agatccttgt tgagtcgcct
     1801 gaccgcttct tcggcccgct tgcgctcggt aacgtcacgg attatggcga gcacgaactg
     1861 ggcttcgcca tcgtctgtgt cgtgcaccgg gcggatggga ctcaaggaca gctcgaccct
     1921 gatctcctgg ccgttcttct tgattgcggg aagatccagc ggcgcattcg agtcgatgta
     1981 gcggccgtgg cccgtcctgg cgtagcgggt tatccccgcc cggtgggcat ccttgagagg
     2041 ttcggggacc agctcttcga cgcgcatcgt cagggcctgg gaggccgggt agccgaacat
     2101 cttcgcggcc gccgcgttcc agagaactat gcgctgcgtc tttgcgtcag cgacgatcac
     2161 ggcgtccctg atcctctcga aaagcctgcc gaagcccaga tcctccggac gcaaccgttc
     2221 acccgcccta acagaacaca tctgaggtcc tcacggtatc cggctgcacc acgggctccc
     2281 cactccctct gagatcttac tcagggttct cgacccttac cccacgggca tcacgttcct
     2341 ggcaaggcag catagcagtc cgttccttcg gacgcatcct ccgatcccag agaatcacta
     2401 ttcgccgctg ccatgtcttg tagggtacgt gtcgacctgc gacgtgctac tgtttagcgg
     2461 tccgacgact atgagccggt agcgcccaac ggggaaagga gcctcatggc cggaactgtg
     2521 gagcgaagac ggcagcacga gtcctccgat gagcgtcagg cagagatcgt cgacgtgctg
     2581 gtggatgctt tttcggggct catggacgcc aaccccgagg cgtttcgcca gaagttcagg
     2641 aagatggccg cggacccttt cgccttctat cgtggcagcg cgtgcctgtt ctacgcggac
     2701 atggaaaggg aggaggaccg ttgggcggat gagcgcacca gccgcgtctg gatccagggc
     2761 gacctgcacg cggagaattt cggcacctac atggacggcg acggggtgtt catcttcgac
     2821 gtcaacgact tcgacgaggc ctacctgggc tacttcacct gggacatcaa gcggatggtc
     2881 gccagcgtgg ccctgctagc ctggatgaag gctatctcgg acgccgacat cgcgtccctg
     2941 atcgaaacct acgtccgggc ttacgttgag caggtgcgct actttgtcga gagtgaccgc
     3001 gaccatgagt
//
LOCUS       DATMVF010000192        11201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_68336, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000192 DATMVF010000000
VERSION     DATMVF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68336"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..879
                     /locus_tag="VN178_09455"
     CDS             85..879
                     /locus_tag="VN178_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81238.1"
                     /translation="MRNGPILASDGRALIVAMDHARTHGAIEGLEDPGAVIEASVDGG
                     ADAVMTTFGVAKRYREQLAGRIPTIIRLDGGPSLYREDWLAYTEWSLLHSVEDALLLG
                     ADGVVLMAFVGIPVELETYRAVARVAGECLRANLPLVVEALPCPSERVPDPKAPDAMA
                     SAARIGFEHGADFVKSYYTEDFRKVTDNCPVPVLIAGGPRMETAKETLQVVQDATEAG
                     AAGVVFGRNIWQSGDTRGMIRALNSIVHGGRPVSEALAELGVRYNL"
     gene            997..2514
                     /locus_tag="VN178_09460"
     CDS             997..2514
                     /locus_tag="VN178_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWS81239.1"
                     /translation="MNPEPILIGADVGTTNIKVVAFDRSGRALEGASSPTPTHFPRPG
                     RAHYDPEELWDTFRATLRRVTQKLEDPGRVASIAVASMGEAAVPLDSGGEPTHDIIAW
                     FDGRAGAQAERLGRIVGQDRLFGLTGLSLQPIFGLCKLLWLKENEPEAYSRTATWLNV
                     ADYMAFRLCGVAATDYSLASRTLALDLHGLRWSGELLEEVGISPALFAPLRGGGSLLG
                     PVTPDAAFATGLPESASVAAGGHDHVCGALAAGVTQKGTMLNSLGTAEAIFLPLERPL
                     ADPQIGHQGYTQGAHVAGQYYVFGGQYTSGASVEWLREALGGVDYDTLIAEAEEVPPG
                     SLGAFFLPQLRLANPPYNDPAARGAFVGLSTDVGRGALFRAVLEGLAYDSRNSLEPLL
                     DHSGIDQLRAIYAIGGGTQNRLLMRIKATVSNQTITVVGVEEATSLGAAILGAIGAGV
                     YADVPSALAELSYAENPVEPTPEEVSFYEAAFRKVYERIYPSLRGVHHEIEALFS"
     gene            complement(2670..2945)
                     /locus_tag="VN178_09465"
     CDS             complement(2670..2945)
                     /locus_tag="VN178_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81240.1"
                     /translation="MMTFGKSRPPLVPDPGQRVILLSEDGSRIEGFRAVSGPITTETG
                     GTIVRVAIEEEYRDSMREGRRAVSMAWPAEMVEVSVPWYKWQRWFTR"
     gene            complement(2964..3614)
                     /locus_tag="VN178_09470"
     CDS             complement(2964..3614)
                     /locus_tag="VN178_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS81241.1"
                     /translation="MRVLIAGAHGKTARRLVRMLAEDDHEVRGLVRKEEQTDDVTADG
                     AEPVVVDLESEEVAGEIGRAVEGCDAIVFAAGAGPGSGAERKETMDYGGAAKLVEAAE
                     ERGVRRYLMLSSMGADDPEGGAEAMRPYLRAKARADERLKGSSLDYTIIRPGSLTEDG
                     GRGTIDAAEKLGRRGEIPREDVARTFADALENDNTYHKTFEILSGETPIPEALGRL"
     gene            3831..4022
                     /locus_tag="VN178_09475"
     CDS             3831..4022
                     /locus_tag="VN178_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81242.1"
                     /translation="MLKCYPGEAVADAEGEAVGDAVEGAVGVDVGGGVGPVVCGGVVF
                     GVVAGGVLGCGLGGSVGVS"
     gene            complement(4224..4382)
                     /locus_tag="VN178_09480"
     CDS             complement(4224..4382)
                     /locus_tag="VN178_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81243.1"
                     /translation="MRIPPRGYLTLALLFGELVCVIVWALTGIFLILVVGIAFGIGSV
                     VAAIRWED"
     gene            complement(4700..4912)
                     /locus_tag="VN178_09485"
     CDS             complement(4700..4912)
                     /locus_tag="VN178_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81244.1"
                     /translation="MGDRRRRLAREDLEAMRQEFVVGEQAGSLDDDLHQIRRSARLGV
                     SPEDWARARKLAPAYARALRRYLDQG"
     gene            5146..6336
                     /locus_tag="VN178_09490"
     CDS             5146..6336
                     /locus_tag="VN178_09490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWS81245.1"
                     /translation="MNLVKGAATSIALLLLLSPVSSPDRAAAQEKTPPGAPKLNAGSW
                     ALIDADSGLYLAGKDPDEQVPIASTTKIMVALVALDEGVDFGEQVTVSEDAAAYAGSI
                     YSNIGLYPYDRVSVGDLLTATLVPSGTDAVYALTEHLGDGSVDEFVARMNDKAEELGL
                     ENTHFENPAGIDARGNYSSAADLATITREAMKHPEFRKIVDDPEATISTQDREIDVFT
                     TNLLVAPGSGLDYGPATGVKTGTSPQAGPCLVASATSGDESYITVVLDAAEDLQRFEA
                     GRTALEYGFGEYESEPLVEREAPYAEVELPYRRGEMVKLVAAKDVSALAGPGLKVERR
                     TTKEEAPPSAETGSKLGTVTVSVEGRNVGSSPLVVQKGYEEASLWQKVTYLAGGLKRW
                     ILSR"
     gene            6607..7335
                     /locus_tag="VN178_09495"
     CDS             6607..7335
                     /locus_tag="VN178_09495"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HWS81246.1"
                     /translation="MAGLDVGQKITYFELPDQQDYPWSLSGQLEMGPVMLIFYSGDWS
                     PYCNGQLASYARRFEEFEKRGAQLAGISVDPPANNANMVGKLLLPFPLLSDPEGELAR
                     LFGLWNVREGVAAPSIVVVDQSGKVRYLYSGTDIADRPRDEEILSALDGLDSGIERLT
                     DGPEFRVGATQAAESSVRPDRRAMDFEQLIPYYRGVYYTTEILGERLGGLGRAGKQAL
                     GELDAFQQLVSSYIEALEETIRLR"
     gene            7593..8600
                     /locus_tag="VN178_09500"
     CDS             7593..8600
                     /locus_tag="VN178_09500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWS81247.1"
                     /translation="MELFIDRSTGRLRALWRLAIQYLAFRLLVLLFFNLLVVAWLLAS
                     SGGQVSSGGLDTLVVPDSPVLPLVSSVAGLVVAILTVWLAGRFLDGRPFSAFGFHLGA
                     GWWLDLLFGMALGALLMTAVFLVELGLGWVEITGSFEARGGAPVVISMLFPIVTFICI
                     GISEELVSRGYQLKNAAEGLNYPSLGPRGAVLLAWVLSSVFFAVLHADNPNATPVSTL
                     NIALAGLMLGFGYVLTGELAIPIGLHITWNFFQGAVYGFPVSGFGAFGPTFLDTRQGG
                     PDLWTGGPFGPEGGLLIPAVMLLGMSLVALWTRLRTGKVSLHTPIAESPYREGPQTPP
                     Q"
     gene            complement(8609..10933)
                     /locus_tag="VN178_09505"
     CDS             complement(8609..10933)
                     /locus_tag="VN178_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HWS81248.1"
                     /translation="MGEFGVVFQNEPVDVSEEFSRQENHFFVGEKVEEFDPQSASGKI
                     CWKGLSLKQRVSYHQITTQFEDYGIWEDLPQGEYEDDRALPFDLSFVTPRTVRLRLAA
                     RPEGCRGEPTLMLEDEPPTDEFWEVSDGGSSVAYRGPCGSVVVDKDPLRFEFRDAAGG
                     LLTSTHNLSDTTGVINARPTPLSFVRNTATFHRHIATSFRLSPGEKLFGGGESFTRLD
                     KRGQKLVLWTYDAYSAQTPNMYKPIPFFMSNRGYGVFVHTSAPLTFDLGRSHDGAAVI
                     YVGDDQLDLFFFFGSPKEILSEYTALTGRAPLPPLWTFGLWMGRQTYSSEREVREVAK
                     KLRRERIPSDVIHLDTGWPEVPHRCDFEFSRPRFADPKKMLADLKEEGFRVSLWQLPY
                     FNPKNRLHREAIEKDFVVLTASGEPPVDDAVIDLSKPEAERWYREKLAGLLEQGVGIF
                     TADFGEAAPLSGVYASRKGGFHEHNLYPLRYNRAVAGATEDVKGNGAIFSRSAWAGSQ
                     RYPLHWGGDAENSDGGMVGTLRGGLSLGLCGFSFWSHFIGGFPYATPEDLYRRWLAFG
                     VLTSHSRCHGRPPTEPWEYDEEFTGDFRRTVELRYRLMPYVYAQAAHACEEGHPMLRT
                     LFFEYPGDKTSWLVEDEYLFGTDILVAPLTEEARSRDVYLPPGLWSDYQGGESYEGAR
                     WHHLHAGEVPVVMLVRDGAVIPHARLAQSTERIEWGEIELKVFGAGDSAEGHFLHPED
                     GDLHVLRLLQEGDGFALEEDPLAGRVNWTISAVP"
BASE COUNT         2006 a         3675 c         3553 g         1967 t
ORIGIN      
        1 gcctgagcgt gctgaattgc tgaaatgctg atagcttcta caaagaggtt caccctcctt
       61 gtaaaagcta tcctgggagt aaaggtgagg aacggaccga tactggcttc cgacggcagg
      121 gcgttgatcg tagcgatgga ccacgcacgc acccacggcg ccatcgaggg gctggaggac
      181 cctggggccg tgatcgaagc ctccgtagat ggtggggccg acgccgttat gacgacgttc
      241 ggcgtggcga agcgctaccg cgagcagctc gcaggacgca tccccaccat catccgcctg
      301 gacggcggcc cgagcctcta ccgggaggac tggctcgcct acacggagtg gagccttctc
      361 cattccgtcg aggacgccct cctcctcggg gccgacgggg tcgtgctgat ggcgttcgtg
      421 ggcatccccg tcgaactcga gacctaccgc gccgtcgcca gggtagcggg cgagtgcctg
      481 cgcgccaatc tgccgctcgt ggtagaggcg ctaccgtgcc cgagcgagcg cgtccccgat
      541 cccaaagcgc cggacgcgat ggcctcggcc gcgcgcatcg gcttcgagca cggcgcggac
      601 ttcgtcaagt cgtattacac ggaggatttc cgcaaggtga cggacaactg ccccgtgccg
      661 gtcctgatag ccggcggtcc gaggatggag accgcaaagg agacgctaca ggtcgtccaa
      721 gacgccacag aagccggcgc cgcgggcgtc gtcttcggcc gcaacatctg gcagagcggt
      781 gacacgcggg gcatgatccg cgccctgaac agcatcgttc acgggggccg ccccgtctcc
      841 gaggcgctcg ccgagttagg agtccgatac aacctgtagg ggcggaccgc ccggagactg
      901 ccgcgcatgg ccatcgacag tatgataagt aacgatgcaa gacggcaccg acgaggatag
      961 gaacggcgac ctgacctcga aaggctctac cccccgatga acccggagcc gatcctgatc
     1021 ggtgccgacg tcggcacgac gaacatcaag gtcgtggcct tcgacaggag cggcagggcg
     1081 ctggaggggg caagctcgcc gaccccgacc cacttcccga gaccgggcag ggcccactac
     1141 gatcccgaag agctgtggga cacctttagg gcgaccttgc gccgggtcac ccagaagctc
     1201 gaagatcccg gccgcgtggc gagcatagcc gtcgccagca tgggtgaggc cgccgtaccg
     1261 ctcgactccg gcggcgagcc cacccacgac atcatcgcct ggttcgacgg acgggcgggg
     1321 gcccaggccg agcggttggg acgcatcgtc ggacaggacc gtctcttcgg cctgacgggc
     1381 ctctcgttgc agcctatctt cggcctgtgc aagctgctgt ggctcaagga gaacgagcct
     1441 gaggcctact cgcgcacagc cacctggctg aacgtggccg actacatggc gttccgtctg
     1501 tgcggcgtcg ccgccacgga ttactccctg gcctcacgca ccctggccct cgacctgcac
     1561 gggctgcggt ggtccgggga gttgctcgag gaggtgggta tctccccggc tctcttcgcc
     1621 cccctccggg gcggcggctc gttactcggt cccgtgaccc cggacgccgc ctttgcaacg
     1681 gggctgccgg agagcgccag cgtcgcggca ggaggccacg atcacgtctg cggcgccctt
     1741 gccgccggcg tcacgcagaa gggcaccatg ctcaactcgc tcggtacggc agaggcgatc
     1801 ttcctgccgc tcgagcgccc gctggcggat ccccagatcg gacaccaggg ttacacccag
     1861 ggagcccacg tggcgggcca gtactacgtc ttcggcggcc agtacacttc gggagccagc
     1921 gtagaatggt tgcgggaggc cctcggcggg gtcgactacg acaccttgat cgccgaggct
     1981 gaggaagtcc cacccggctc cctcggcgcc ttcttccttc cacaactgcg cctcgcaaac
     2041 ccaccgtata acgatcccgc agcgagagga gcgttcgtcg gcctcagcac ggacgtgggt
     2101 cgcggagccc tcttccgggc cgtactcgaa ggactggcct acgactcccg caactcgctc
     2161 gaaccactac tcgaccactc tggtatagac cagctcaggg ccatctacgc catcggcggc
     2221 ggcacccaaa accgactgct gatgcgcatc aaagccaccg tctcgaacca gacgataacc
     2281 gtagtcgggg tagaggaggc gacgagtttg ggggccgcca tcctgggcgc gatcggcgcc
     2341 ggcgtctacg ccgacgttcc atcggccctc gcagagctga gctacgccga gaaccccgtc
     2401 gagccaaccc cagaggaggt ctccttctac gaggccgcct tccgcaaagt ttacgagaga
     2461 atctacccca gcctccgcgg cgtgcaccac gagatcgaag cccttttcag ctagtcagca
     2521 cgtcgccagg cttcgccagg cgacttctca gcatttcagc tagtcagctt tcatatgcgc
     2581 aggtcctggc tgacaaggcg ccaacggcgc cgtgctgaaa tgctgacaag cgacagccgc
     2641 aggctggagc gtgctgaatt gctgaaatgc tacctcgtaa accaccgctg ccacttgtac
     2701 cagggcacag acacttccac catctcggcg ggccatgcca tgcttacggc gcggcggccc
     2761 tccctcatcg aatccctgta ttcctcctcg atcgccaccc ttacgattgt cccacccgtc
     2821 tccgtagtga tcggtcccga gacggcacgg aatccttcga tgcgcgagcc gtcttcggag
     2881 agcagtatta cccgctgtcc agggtcgggt acgagtggtg gcctactctt gccgaacgtc
     2941 atcacttcac cgatcgtatc cgtctagagc cgtcccagcg cctcggggat aggggtctct
     3001 ccggagagga tctcgaacgt cttgtggtat gtgttgtcgt tttcgagcgc atcggcgaag
     3061 gtccgggcga cgtcttcgcg ggggatctcg ccccgccgcc ccagcttctc ggccgcatct
     3121 atcgtgcccc tacccccatc ctcggtcagg ctgccgggac gtatgatggt gtagtcgaga
     3181 ctactgccct tcagacgttc gtcagcgcgg gccttggcac ggagataagg acgcatcgcc
     3241 tccgcgcctc cctcggggtc gtccgcgccc atagagctga gcatcaggta gcggcgcacc
     3301 ccacgttcct ccgcggcctc gaccagcttg gcggccccgc cgtagtccat agtctccttg
     3361 cgctcggccc cgctccccgg tccggcgccg gcggcgaaga cgattgcgtc gcatccctcg
     3421 accgccctcc cgatctcccc tgcgacctcc tcgctttcca ggtcgactac caccggctcg
     3481 gcgccgtcgg ccgtgacatc gtcagtctgc tcctccttgc ggacgagtcc cctgacctcg
     3541 tggtcgtctt cggccagcat ccgtaccaga cgcctcgccg tcttgccgtg cgcgccagcg
     3601 atcaaaaccc tcacggaact acctccttcg attcagcacg gctgctccgc cgccttatca
     3661 gcatatcagc acgctccgcc ctgcgggctc cgcttgtcag catttcaggt cccgcagtcg
     3721 aagcggatcc tcaatgagcg ggtgctcccc taggattctc tcgagcgtct cgccacacta
     3781 cactcagctg acatgctgaa gtgctgacaa gcgaaggcgc aagcctgagc gtgctgaaat
     3841 gctatccggg ggaggccgtc gcggacgcgg agggtgaagc ggtcggggat gccgttgagg
     3901 gcgccgtcgg ggtcgatgtg ggaggtgggg tcgggccggt cgtctgcggg ggcgtggtct
     3961 tcggggtagt cgccggcgga gtcctgggct gcgggctcgg aggatcggtc ggcgtttcgt
     4021 agaacgggga cagcagcgcg gacaatatga cggcaacggc cacagcggcg acggtgcaca
     4081 cgggcgcgag aacgagggcc agaggcgctc gcaggccctc ggacaccctt ccgcgcagca
     4141 tgtagtacag gagcccggcg aaaacgccga gcacggagat caagagccac aaagggtatc
     4201 cgaggtcgag cccacccacg cttctaatcc tcccagcgta tcgctgccac tactgaacca
     4261 atgccgaagg cgatgccgac gacgagtatg agaaagatac ctgtaagagc ccagactata
     4321 acgcagacga gctctccaaa gagcaacgcc agcgtcaggt atcccctcgg cgggatcctc
     4381 atctggtctc cactccttct ctgcggtgga ttacagggcg cagcctagag aataaacgtc
     4441 cctcccacga tcgagtgtat tgtagtacgg aagtccttct tgcgggtcgg gagaatccgg
     4501 gatgcagcgg agtaggatcg ggccggtaag gagggggaag gtaccgaaga cccgatccta
     4561 cgggtaatgt cgccgggcct cgggggtcct gagaggatgc cgcgcccggt gttgtactac
     4621 tctacgacac caggagcagg cggcccatca ctcaaatgag tgatattctg cgcgatccga
     4681 aaagtttctg gcgtcgaccc tatccctgat ccaggtacct ccgaagcgcc ctggcgtaag
     4741 ccggcgccag cttacgcgcc ctggcccagt cttcggggct cacgccgagc ctggcgctgc
     4801 gccggatctg atggaggtcg tcgtccaggc tgccggcctg ctcgccgact acgaactcct
     4861 ggcgcatggc ctccaagtcc tctctggcca acctcctccg acggtctccc atgcgtcctc
     4921 ctctcgccga aagagcacgg agtgccctaa gcctaacccc accccatcgc agcggcaacg
     4981 gctgatgctc tgaatattct ccacaatccc gcaagatcga cggtctccgg ggtggtaaga
     5041 gagggtcctc tcgtgcaaaa tatccccgtt tgcctccggc ggcaccgtcg tagggggagc
     5101 acgaccggtt atcgacgtag aggagagcca cgggtgcgag caagaatgaa cctagtcaaa
     5161 ggagcggcga cctccatagc gctcttgctg ctcctttcgc cggtgtcttc gccggatcgg
     5221 gccgccgccc aggagaagac gccccccggg gccccgaagc tgaacgccgg ttcctgggcg
     5281 ctcatcgacg ccgactcggg tctctacctg gcgggcaagg acccggacga gcaggtaccc
     5341 atagcctcga cgaccaagat catggtcgca ctggtggcat tggatgaggg tgtcgacttc
     5401 ggggagcagg taacggtctc ggaggacgcg gccgcatacg ccggctccat ctacagtaac
     5461 atcggcctct acccctacga ccgcgtgagc gtcggggacc ttctcaccgc gaccctggtc
     5521 ccctccggca ccgacgcggt ctacgccctg accgagcatc tgggggacgg tagcgtggac
     5581 gaattcgtcg ccaggatgaa cgataaggcg gaggagttgg gcctcgagaa cacccatttc
     5641 gagaaccccg cgggcatcga cgcccgcggt aactattcga gcgccgcaga cctcgcgact
     5701 ataaccaggg aggcgatgaa gcaccccgag ttccgcaaga tcgtcgacga tcccgaggcc
     5761 accatttcga cgcaagacag ggagatagac gtcttcacca ccaacctgct cgtcgccccg
     5821 gggtctggcc tggactacgg gccggcgacg ggcgtcaaga cgggcacctc tccccaggcc
     5881 gggccctgcc tcgtggcctc cgcaacgagc ggcgacgagt cctacatcac cgtggtgctc
     5941 gacgccgcag aggacctgca acgtttcgag gcaggccgga cggcgctcga gtacggcttc
     6001 ggcgagtacg aaagcgagcc tctcgtggaa cgcgaagccc cttacgcgga ggtggaactt
     6061 ccctaccgca ggggcgagat ggtgaagctg gtcgccgcaa aagacgtatc cgcgctcgcc
     6121 ggcccaggct tgaaggtcga gcggcgcacg acgaaagagg aggcgccgcc ctccgccgag
     6181 acagggagta agctgggtac cgtcacggtc tctgtggagg gccgcaacgt gggtagcagc
     6241 cccctggtcg tccagaaagg ctacgaagag gcctccctgt ggcagaaggt gacgtacctg
     6301 gccggggggc tgaagcgctg gatcctatcc cgctgatagc gggatgaggg agggttgccc
     6361 ctccctcatc ccgcgcgagt cagcacgccc gcttcgcggg cttgtcagca cttcagcttt
     6421 cgtatgtgca cgcccttgct gacatgctga caagcgacag ccgcaggctg gagcgtgccg
     6481 acaagcgaag gcgctagcct gagcgtgctg actagctgaa atgctggcca gctgccggtt
     6541 ccgcgtcctc cggcgcctac gatgctatat tcccagcctg gaggaccaca gagggaggag
     6601 tatcacgtgg ccggattgga cgtcgggcag aagatcacat acttcgagtt gccggatcag
     6661 caagactatc cctggagcct ctcgggccag ttggagatgg gacctgtgat gctgatcttc
     6721 tacagtggcg actggtcacc ctattgcaac gggcaactgg ccagctacgc caggagattc
     6781 gaagagttcg agaagcgggg ggcgcagttg gccggcataa gcgtcgaccc tccggccaac
     6841 aacgcgaaca tggtcgggaa actcttgctt cctttccccc tcctcagcga ccctgaggga
     6901 gagctcgccc gtctgttcgg cctgtggaac gtaagggagg gcgttgccgc gccttccatc
     6961 gtcgtcgtag atcagtcggg gaaggtacgc tacctctact cgggcacgga catcgcggac
     7021 aggccgcgag acgaggagat cctctctgcc ctcgacgggc tcgacagcgg gatagaacgc
     7081 ctcacggacg gaccggagtt ccgggtagga gcgacgcagg cggccgagag cagcgtccgc
     7141 ccggatcggc gcgccatgga tttcgagcag ctcatcccct actatcgggg cgtctactac
     7201 accacagaga tcctggggga gaggttgggg ggattgggcc gcgccggaaa gcaggcgctc
     7261 ggggaactgg acgcattcca acagctggtt agctcttaca tcgaagccct ggaagagacg
     7321 ataaggttac gctagcacgc tccagccttc ggcttccgct cgtcagcatt tcagcacgcc
     7381 gcctccctcc gtccggcggc ttgtcagcac ttcagcaggg tggtaggcct ccctcaaggc
     7441 tcggagagaa gccccgagtg atatgcttca cgcgacgggg cgagtgctcg aacggacgaa
     7501 gctgacatgc tgacaagcga gggcctgtaa ggcccgaagc gtgctgacaa ggcgcgaagc
     7561 gccgtgctga ctagctgatg cgcggaggct gcttggaact gtttatcgat aggagtacgg
     7621 gacggctcag ggcgctgtgg cggttggcga tccagtacct ggccttcagg ctactcgttc
     7681 tactcttctt caacttgctc gtggtcgcgt ggctgcttgc gagttccggc ggtcaggtct
     7741 catccggcgg actcgatacc ctggtcgtcc cagattcccc cgtgctcccg ctcgtcagca
     7801 gcgtagccgg cctggtggtg gctatactga ccgtgtggct cgcgggacgc ttcctcgacg
     7861 ggcggccctt ctccgcattc gggttccatc tgggcgccgg atggtggctc gacctgctgt
     7921 tcggtatggc gctcggcgcg ctgctcatga cggccgtgtt cctcgtcgag ctggggcttg
     7981 gttgggtgga gatcacaggc tcgttcgagg cccgaggcgg tgctccggtc gtgatctcca
     8041 tgctcttccc catcgttacg ttcatctgca ttggcatctc cgaggagctg gtctcccggg
     8101 gctaccagct caagaacgcc gccgagggcc tgaactatcc ttccctcggt ccccgcgggg
     8161 ccgtcttgct ggcgtgggtc ctctcctcgg tcttcttcgc cgtgctacac gccgacaacc
     8221 cgaacgccac gcccgtaagc acgctcaaca tcgccctcgc gggcctgatg ctaggcttcg
     8281 gctacgtgct caccggagag ctggcgatcc cgatagggtt gcacatcacc tggaacttct
     8341 tccagggcgc agtctacggc ttccccgtca gcggcttcgg agcgttcggt ccgaccttcc
     8401 tggacacgcg ccaggggggt cctgatctct ggaccggtgg tcctttcggc cccgaaggtg
     8461 gcttgctgat ccccgctgtc atgctactag gaatgtccct ggtcgcgctc tggacacgcc
     8521 tgcgcaccgg caaggtctca ctccataccc caatagcgga aagcccgtac cgcgagggcc
     8581 cacagacccc accccagtaa cccgaagttc atgggacagc gctaatcgtc cagttcaccc
     8641 tgccggcgag gggatcctcc tccaacgcga aaccgtcgcc ctcttgcagg aggcgcagaa
     8701 cgtgcaagtc gccatcttcg gggtggagga agtgtccctc ggcggagtcc ccggcgccga
     8761 agaccttgag ttcgatctca ccccactcga tacgttccgt gctctgcgcg agcctggcgt
     8821 gtgggatcac cgcgccgtcc cttacgagca tcacgaccgg gacctcgccc gcatgcaggt
     8881 ggtgccagcg ggcgccctcg tacgactcgc cgccctggta gtcgctccag agccccggtg
     8941 gaaggtagac gtcgcgactc ctcgcctcct ccgtcaacgg cgcgacgagg atatccgtgc
     9001 cgaagaggta ctcgtcctcc accagccagg aagtcttatc acccggatac tcgaagaaca
     9061 gggtacgcaa catgggatgt ccctcttcgc aggcgtgcgc ggcctgggcg tagacgtagg
     9121 gcatgagccg gtacctgagc tcgaccgttc gtctgaagtc gcctgtgaac tcctcgtcgt
     9181 actcccacgg ctcggtgggg ggcctgccgt ggcacctgct gtgcgaggtg agcacgccga
     9241 acgccagcca gcgccggtag aggtcctccg gggtcgcgta ggggaacccc cctataaagt
     9301 ggctccagaa cgagaacccg cagagtccta gagagagtcc acccctcagc gtccctacca
     9361 ttccaccgtc cgagttctcc gcgtcccctc cccagtgcaa ggggtagcgt tggctgccgg
     9421 cccaggcgct gcgcgagaag atagcaccgt tgcctttgac gtcttcggta gccccggcca
     9481 cggccctgtt gtaacgcaga gggtaaaggt tgtgctcgtg gaagccgccc ttcctggagg
     9541 cgtacacgcc cgagagcggc gcggcctcgc cgaagtccgc cgtgaagatc ccgacgcctt
     9601 gctccagcag tccggcgagc ttctcgcggt accaacgctc ggcctcgggc ttgctcaggt
     9661 cgatgacggc gtcgtcgacc ggcggctctc cgctggcggt aaggaccaca aagtctttct
     9721 ctatggcctc gcggtgcagc ctgttcttgg ggttgaagta agggagttgc cacaggctga
     9781 cgcggaagcc ttcctccttc aggtcggcga gcatcttctt cgggtccgca aagcgggggc
     9841 gggagaactc gaagtcgcag cggtggggaa cctcgggcca gcccgtgtcg aggtggatca
     9901 catccgaggg gatgcgttcc cggcgcagct tcttcgcgac ctcgcggacc tctctctccg
     9961 aggagtaggt ctggcggccc atccagagcc cgaaggtcca aagtgggggg agcggggcac
    10021 gtcccgtgag cgcggtgtat tcggagagga tctccttcgg ggagccgaag aagaagaaca
    10081 ggtcgagctg gtcgtcgccc acatagatta ccgccgcccc gtcgtgcgag cgcccgaggt
    10141 cgaacgtgag cggcgcgctg gtatgcacga agacgccgta gccacgattg ctcataaaga
    10201 aagggatggg cttgtacatg ttgggtgtct gggcgctgta tgcgtcgtag gtccagagca
    10261 ccagcttctg gccgcgcttg tccaggcggg taaaggactc cccgccaccg aagagcttct
    10321 ccccgggcga gagtctgaag ctggtggcga tgtgcctgtg gaaggtggcc gtgttgcgta
    10381 cgaacgagag gggtgtcggc ctggcgttga tcacgccggt ggtgtcggag agattgtggg
    10441 tgctggtcag tagaccaccc gcggcgtccc tgaactcgaa gcgcagcgga tccttatcca
    10501 ccaccaccga gccgcaagga ccacggtatg ccacggagga tcccccatcg ctcacttccc
    10561 aaaactcatc ggtcggaggc tcgtcttcga gcataagcgt cggttctccg cggcatcctt
    10621 ccggcctggc ggccagcctg agacgcaccg tgcgcggggt cacgaaagag aggtcgaagg
    10681 ggagggcgcg gtcgtcctcg tactctccct gcgggaggtc ttcccagatc ccgtagtcct
    10741 cgaactgggt cgtgatctga tggtaggaga ccctctgctt gagcgagagg cccttccagc
    10801 atatcttccc ggaggcgctc tgcgggtcga actcctcgac cttctcgccg acgaagaagt
    10861 ggttctcctg cctggagaac tcctcgctca cgtccaccgg ctcgttctga aagacaaccc
    10921 cgaactctcc cataagcgcc ttcctccacg tgagaacgtc tctttcctac gacaacctac
    10981 attatccgcg ttctcgatac cgggtgccgt aggaggcttg caacctttac ggtttgatgc
    11041 ggcaagaaaa ggggcaagtg accacgtgaa acgagtgaaa cggccgcagg gaaggacgac
    11101 caggatgcca gaagaccagc aaaggcaaca ggacccgacg cagcagtatc cgcagccgga
    11161 gtacccggag cagacccaga tggaccagca tcccggcctg g
//
LOCUS       DATMVF010000193        31118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_434235, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000193 DATMVF010000000
VERSION     DATMVF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 31118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31118
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434235"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN178_09510"
     CDS             <1..322
                     /locus_tag="VN178_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS81249.1"
                     /translation="EEMVADGYRQFAVRHEAGELVAVAGVAVHVNLYYGRHLYLYDLV
                     VTEGARSKGYGESLLDHVEGIARREGCETAALACGLEREGALRLYERRGYQKPSYAMR
                     KALR"
     gene            569..790
                     /locus_tag="VN178_09515"
     CDS             569..790
                     /locus_tag="VN178_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81250.1"
                     /translation="MLLLFFGEPVLLIIVYAAMGALFLPFLTITLLWLLNSRRVAPEH
                     RNGIVSNIILVASVLLFVFLGIQEVLDAA"
     gene            complement(872..1687)
                     /locus_tag="VN178_09520"
     CDS             complement(872..1687)
                     /locus_tag="VN178_09520"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HWS81251.1"
                     /translation="MNLRKPSAPAFDAFIARLQLRLNARATAATREWWTKYLRGTASF
                     RGVKMGDVRAAVHTWFEEEQLGKLLSIEQQKDLALTLLEEDHAEDKLAGVLFLQEILL
                     SEGALDWRSDLSRFARLFDEGYIRDWSICDWLCVKVLGPLVEQQGEPCARAISEWRGT
                     QSVWQRRASVVAFANLARKGDQNFAGFTEMILNDCSRLLESQERFAQTGVGWVLRELS
                     RSDQGRVIGFVEANLDRFSREALKNAIKYLPPEVKERLLRAHLQSGTRGLRQR"
     gene            complement(2076..2459)
                     /locus_tag="VN178_09525"
     CDS             complement(2076..2459)
                     /locus_tag="VN178_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81252.1"
                     /translation="MEHRLELAAKALAIERVVTGGLAAIMPGRAIGAIMTPGADTGAA
                     RYVARLLGIRQIVLGVMLWQARDDRAWLRRMATLNALMEALDALATLVDLVQQRGGDR
                     PSAATVGAALSASAAFLALRSAAAS"
     gene            2780..3031
                     /locus_tag="VN178_09530"
     CDS             2780..3031
                     /locus_tag="VN178_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81253.1"
                     /translation="MSEQHTEQTIKVRQITDVHANWSSQGPLENGKFSYQLILDNGAE
                     EALIMPTADDAKVLRDLIHDADTLYWDTDKEIIIFGKVD"
     gene            3241..3477
                     /locus_tag="VN178_09535"
     CDS             3241..3477
                     /locus_tag="VN178_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HWS81254.1"
                     /translation="MILVDPIRRHRSGEWCHMVSDESVGELHEFAATIGLKRKSFQST
                     SRPHYDLRPSKRRLAVSMGAQQVGMRDLVRRAVR"
     gene            3663..3965
                     /locus_tag="VN178_09540"
     CDS             3663..3965
                     /locus_tag="VN178_09540"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWS81255.1"
                     /translation="MFAAIRYYQADPPSIDEVVRRVQEEFVPLIRDMRGFVSYFILVP
                     SEREEDIVSVSVFEDQQSAEESNRKAAEWVVQNLSELLRPTPEFASGQVVVYEASQ"
     gene            4323..4718
                     /locus_tag="VN178_09545"
     CDS             4323..4718
                     /locus_tag="VN178_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81256.1"
                     /translation="MEQTEHGDQPTCGEGLAANSILPAKLAELMAAQAEVLERHTRAL
                     DLADATAQKELDAYAKLERAHRDVASELADLGEAMAGYRDLPMGRHDMTVMTDPEGQM
                     EAFRRFVAVERELLALLQTKLEAEEELLQ"
     gene            4740..5252
                     /locus_tag="VN178_09550"
     CDS             4740..5252
                     /locus_tag="VN178_09550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWS81257.1"
                     /translation="MCHTRAPGVREPHPTQPCRLPRSPRGTPCAALSQLAWAHAHEIL
                     LCASKGGGSQCTFNYYLINSRDPASQVSSVWHIPSVLRREKRHGYHHTQKPLRLLRRA
                     ILASSREGDLVFDPFCGSGTSGVASKELDRFFVGTEKEEEYAELAARRIDAAVRGEVL
                     REIEEARPAV"
     gene            complement(5548..5790)
                     /locus_tag="VN178_09555"
     CDS             complement(5548..5790)
                     /locus_tag="VN178_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWS81258.1"
                     /translation="MSKALLFSTSTCSWCRRAKRYFKERGVPFKEINIERDADAARDI
                     VRKTGQTGVPVIKIGSRWIVGFDKDQIDRELARKAS"
     gene            6080..6343
                     /locus_tag="VN178_09560"
     CDS             6080..6343
                     /locus_tag="VN178_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81259.1"
                     /translation="MTSGYTPGSMPGKDQPGFDQILRALREGLNENSRLKEMTAEEVA
                     RQLVQGGHLEVEPPPPLVAEALEGLEAEEQSLQNEELSSEEAN"
     gene            6391..6534
                     /locus_tag="VN178_09565"
     CDS             6391..6534
                     /locus_tag="VN178_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81260.1"
                     /translation="MLIDGFRLLQPDLVAFQEAVVRDGYDQVTDILGTGHHVAGVVAE
                     LSA"
     gene            complement(6593..7879)
                     /locus_tag="VN178_09570"
     CDS             complement(6593..7879)
                     /locus_tag="VN178_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HWS81261.1"
                     /translation="MSERAPLRVVATHDFCCAYPASPTSYGSLPGGEGLKNMVEVLRA
                     QGSIVRADAGDFAAPGALATLSNGKAGFAAAADLGIDVGVVGNHEFEWGMEHLRAHAP
                     ETGFPLLCANAPEVGLPPTAMVETEAGPVGFVGLTCPDPGVYVSAPSLDPDLAGVAIR
                     HAEELRDAGAKWVVALLHDGVDWRFGARGYEANPERFSEVCRPWAHAVDAIFASHTLG
                     RWIGQVKGTPVVQPWPFGAELGVVELEVGEEPRAYCMTPKTGGRWSGAGSELLDEAST
                     RVLGELLEPLSSRSGGPAPLADFFARALREATGVRAAGVDMLASQPPVDGGLFYLPAG
                     PVTEADVLRLYPWPDATVAGEVEGEELRAVARSAWPKPQAAWGFDAADIAGPGVATLV
                     VLEGDAVAHVERTLGRRVEWRRTGVGLREAVGRKLS"
     gene            complement(8007..9170)
                     /locus_tag="VN178_09575"
     CDS             complement(8007..9170)
                     /locus_tag="VN178_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWS81262.1"
                     /translation="MTSDRLDAEIRPFRIDIPQADLDDLADRLSRTRWANELPLGASG
                     PQTGPVPPGWEYGVPLDYVKNLVGYWQTGYDWRSWEAKLNSYPQFTTTIDGQNVHFLH
                     VRSPEPDALPLILTHGWPNTVVEYLGLIGPLTDPHVHGGDSADAFDVVIPSLPGFGFS
                     GPTAEAGWDAQRTAKAWAELMRRLGYDRYGAHGNDAGAIVAPLQGRLDPEHVIGVHVN
                     QLFSFPSGDPKEFEGLSHEDMEKLRFLQAFNDEMSAYARLQESKPQNLAHALADSPAG
                     QLAWSGQLFGDALSPDEILTNVSIYWLTNTAASAARFYYENKHAEHPTGPTTAPIGLA
                     SFAYDFRPVRRFAERDHTNIVSWSEYDRGSHWAAHDAPDLLVGDIRQFFRRFR"
     gene            9443..10117
                     /locus_tag="VN178_09580"
     CDS             9443..10117
                     /locus_tag="VN178_09580"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS81263.1"
                     /translation="MSGRRAEARRNDRKILEAARAVFVEDPDAPISKVAERAGVGISA
                     LYRRYASKEELLRRLCSEGLRRYIAEAEAALADEDDPWVPFADFMCRIVDADTHSLTL
                     RLAGTFAPTEELYQDSRKAQELNLRLLERTKTAGAIRHDIEVDDLSLLFEQVAAVRIG
                     SEERTRQLRRRYLALQLDALRIPSGSPLPGPPPSWEEISRRWENQPSTRSSEARGKGS
                     PLSGRQ"
     gene            10131..10376
                     /locus_tag="VN178_09585"
     CDS             10131..10376
                     /locus_tag="VN178_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HWS81264.1"
                     /translation="MASSREVDLVFDPFCGSGTTRVASKELDRFFVGAEKEEVYAELA
                     ARRMGASEREGVFERSPTSARETPDSRENRSRTAFSR"
     gene            10418..12211
                     /locus_tag="VN178_09590"
     CDS             10418..12211
                     /locus_tag="VN178_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWS81265.1"
                     /translation="MGELIGVLRRTAAPAYGVAVVAAFVFCPALAAAQGSDTVYDEAG
                     ALSGPEEQRVQQAFDSAQEETGQPLYAFLVPNKGVEGQEARQELLTREATEAQVPQDA
                     GVVVVATNDGWGTTYNFPQDAYNTMVPDFREGDFAAGLVTGAREIQGEPAAQNAQNEP
                     DAGTGGLLGGGLLLLAAVAGSLLLFRNRRAKKRRMEEERRSAEEEFAGLTVLLNEFDE
                     KERLTSGYLEAQRSLLDQRTEEWIETKISDAKASGFAREFDEAASRLTSDPIRARGRL
                     EHGRNLLAESMQQLHEAERTMDDYRAADEALDGRLHVAKEEIQAAEAAEETARAEGVA
                     VEPLKLRPEYDRVAREAADRASRRDEFDPRHSLAAVETLTERARGHRKALQNEISARD
                     ALPDERYATEGGLLRARETLEEYRGAHERALGEFGQAALGEVPNPGELSSGLLEAQGC
                     MDRAARAASSGRFGEARSLLQGAAEIARKTTQSPTRLKAATAEADRKKREGEEKLQEL
                     EARLAQAKANEHLMDPRQRRRLREYEYQLENARYGFFGADWLTALLLFEALDNDYMYL
                     GDPSSFGGGDWGGGDWGGGDFGGGDWGGGDF"
     gene            12981..13553
                     /locus_tag="VN178_09595"
     CDS             12981..13553
                     /locus_tag="VN178_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWS81266.1"
                     /translation="MRVLVDRTHSLSPDYVPEDLVALQDYGVPTLGSDVLRLRRAAAE
                     NLGHLVEDSEADGEELVVASAYRSYEEQQLSHERLISIFGADAGWMSAQPGHSQHQLG
                     TAIDFTNAAAGYQVWMPFAQTSAYGWLERHAWEYGFILAYPKGKEEQTGYQWEPWHYR
                     YVGVDEAKRLEKKNLSLQEFLEREGTMSHC"
     gene            13751..14725
                     /locus_tag="VN178_09600"
     CDS             13751..14725
                     /locus_tag="VN178_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81267.1"
                     /translation="MRGMEGMMRRTVLGIATTMVAATFAACGLAMLAEEEPAQAAYPG
                     QNDRIAYVSHSPSGLHEIYTMNPDGTDRKKLTDNQAYDTSPTYSPNGKEIAFVSQREG
                     QESGKVYTMNAEDGSGQRNLSGSTDGRDPTYSPNGGRIAFVGQGENEDILVMSARDGS
                     AQKNLTNTPPVNGYEVRERDPAYSPDGTRIVFYKETFRPHDVDLYKLNMKRTGTKPAI
                     LQITDFPGKEYSPDWSPNGNRIVFYQDTSAKGLRIFTIRPDGTNRQLVVQDGKDPVFS
                     PDGRKIAFTRDGSVYTVNADGTDERRVPGTPTENGPSEVLPNWGPKSQ"
     gene            complement(14816..15649)
                     /locus_tag="VN178_09605"
     CDS             complement(14816..15649)
                     /locus_tag="VN178_09605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWS81268.1"
                     /translation="MANALALPHILEIAFDVLTAAVGGLVILLALRVAPALTLSAHRL
                     ALRISIVAAVLITAAQLAEVLAVLSRLSTVEDAAADVAELIAVCFVGLALHQMGRAER
                     VEISPLRRAADVDHLTGLVSRSFFHRAAERRMELYKRNGLPLACAVLDVDDFKSYNDR
                     YGHGNGDEALRCVGRVLRESTRADDVVSRYGGEEFVVLMGGNIEDAIEVAERVRQGVE
                     HESVTENGILLGSVVTVSVGVAALTEDVSSLEQLVEKADDELYRAKRAGKNRVAAAGR
                     R"
     gene            15891..16160
                     /locus_tag="VN178_09610"
     CDS             15891..16160
                     /locus_tag="VN178_09610"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWS81269.1"
                     /translation="MCRHIKTLFNFSPPATDEEINAASLQYVRKITGFNKPSKTNEEA
                     FYSAVEDIARISERLLASLETSAPTKDREEEAAKAKARSALRFGG"
     gene            complement(16306..16584)
                     /locus_tag="VN178_09615"
     CDS             complement(16306..16584)
                     /locus_tag="VN178_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81270.1"
                     /translation="MPANPDPGLSYRQEFYKNEAEDKGAVITVGEERGQVPFGYFDKN
                     ILMTRDLVPTEPKVQELKFYAPNVGPLLSVHTDGDGSRAELIRYSRNG"
     gene            complement(16705..17127)
                     /locus_tag="VN178_09620"
     CDS             complement(16705..17127)
                     /locus_tag="VN178_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81271.1"
                     /translation="MEGKRTRPLMRGVVALALAVGLALVGACGTSTTAEKGESPGSTT
                     GEASGSGGRGGMPQGSEPVKLDPADFSVSIDNPYWPMSPGSKWVYRETDSKGTNQKVV
                     VEVTDKTKMIANGIEARVIRDTVTENGSPVEITEDWYA"
     gene            17640..18020
                     /locus_tag="VN178_09625"
     CDS             17640..18020
                     /locus_tag="VN178_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81272.1"
                     /translation="MALGALGGDARSGVERVSEAVYRAMFEAVVVTMTRVPHVLAIMA
                     AVVLFYLAYRRRPGPPKWGMWPGIFAVLALYGFLAALAPAGWDEPLVLLGIGFAALSG
                     YAWLDPDRRRHSAGAGQTSRRRGW"
     gene            18103..19710
                     /locus_tag="VN178_09630"
     CDS             18103..19710
                     /locus_tag="VN178_09630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWS81273.1"
                     /translation="MMALPEHSPGTLRTPVMMALIAVMISLFPLLFGCSSEGPKDQSD
                     GSRRTDENRPAGATPGGQTTGDGSKGARRTETQAALVPIAHLSSTAENISTQELSETR
                     DLAVGREYRDEAEELVDSPGFESFDSGAAVVDHVSKTPDALGLVPWGEVGPRVKALSV
                     DGRSLLDSEGGGDYSLAPEGATVPDPQELRRVVVGGDIVFDRGQYYMVIQRGMGIDFP
                     LGGGYAAVTGRVPEQSIYSETGIIHQFTARRTGRAGAVREYLSGADLTLANFENPVIR
                     DSVYHPDATTFTGDLRLMPVLDQAGMDGVTLGNNHILDAGTTGLDETMRHLDDAGIAH
                     AGAGMDLAEARKPMVFDLGKMKIGVLSYLDVPSYGWAWATQTAPGTAPLLQNVIKEDI
                     ERLRPRVDLILVMPHWGKEYIATPEPQQVDLAHAAIDAGADLVVGGHAHWPKGIELYE
                     GKPIFYGVGNFLLDQSWSEETSTGIFAEITLHGDDVAQVRPVPFIMLDRAQPNFLMPG
                     AGGDRTLRKIFAASIGPEFETYRRRAR"
     gene            19902..20315
                     /locus_tag="VN178_09635"
     CDS             19902..20315
                     /locus_tag="VN178_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS81274.1"
                     /translation="MHEQENLKTIHQVYAAFGEGDIEGVLGMLTDDVRWSTPGPPDVI
                     PYAGLRTGHDQVAGYFKSFGEAVETTAFEPQRFFAQDDMVVVLGRYALRVVSTGNAID
                     NDWVHTFKLAEGKISAFEGYEDSAAVVAAFTAKPH"
     gene            20438..20917
                     /locus_tag="VN178_09640"
     CDS             20438..20917
                     /locus_tag="VN178_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWS81275.1"
                     /translation="MSLAVVIADTHLPRRASALPEVLIPQLKRADLILHAGDLMVPKL
                     LEDLAAYAPVRAVRGNLDPPDVRLPETVQFEFGGVSIAMIHDSGRKKGRRSRMRRRFP
                     EARVVVFGHSHIPWLEDAEGLLLLNPGSPTDRRRQPEHTFALLCVEGGEVRAEVLAL"
     gene            20965..22035
                     /locus_tag="VN178_09645"
     CDS             20965..22035
                     /locus_tag="VN178_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HWS81276.1"
                     /translation="MADDEHRRSENRVFLERRSYEPQLALRRVESAVLALVIITVVGI
                     LGYMVLEGWSFTDSLYMTVITLTTVGYKEVRVLDTSGQLWTMLLLITGVGTLFYAAVS
                     SVELVVEGTVRGYVGRRRVKAAIGRLNGHYILCGYGRVGKQVAREFVADDVPFVVVEQ
                     DQDILEECLAEGNLALLGEASDDEVLEEAGILRAGGLVAAVDSDADNVFVVLSARKLN
                     PKLHIVARASSEELADKLEIAGADRTLSPYAVGGRRLASLATQPLVVDFLDVVTRGEK
                     GIEFHLEEYFVPEDSTIAGHTIGELEIGERTGAIVLAISTTEGTFDTTPSADVRLRAG
                     DTLIVLGSRGQTERLEHLMRGT"
     gene            complement(22075..23481)
                     /locus_tag="VN178_09650"
     CDS             complement(22075..23481)
                     /locus_tag="VN178_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81277.1"
                     /translation="MLLIAQGLAALLFLRHGWDALAFPYPLNYGEGPLLDQSVRLAQF
                     QNIYRTDLAVSPYVISNYPPLFMLTQVPLVWLFGPALWYGRLISLLSVVAVALFVGLT
                     LHALTKDRIAAIAGGLIFPAIPYVVRWSSLSRVDSLGLALSWAGLFVVARWPEKRWSV
                     FVSALLLVAAVYTRQTYILAAPLAAFVWLAAQGQRRRALETAAIAGGAGLIVFALLNI
                     FTGGGFFLNTVTANLNDFRWERVSLNALGALLACPLLLLGSLAFLVLTPREGNRSWWL
                     VAPYLLASIPSAVLVGKVGSDVNYLLELSAALGLATGAFIAWQRRRPRLRIALIALLA
                     IQVLALAQSSRVASGLQDYVIEQRGEVAQLSRMVAGADGPVLTDDYMGLLPQQGRRIY
                     FQPFEMTQLTLDGDWDQRPILKDIDQQKFSMIMIWSPSFAREIKRDRWTPRMLEEIHD
                     SYQRTDRVADMVIYRPRE"
     gene            23740..25356
                     /locus_tag="VN178_09655"
     CDS             23740..25356
                     /locus_tag="VN178_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWS81278.1"
                     /translation="METLGEQARDLTEGIAREASEQFRIDARDFLSARERGGTTGTRI
                     DQQTAALSDAAEIVNRSFPTLEPSGRFINAISPVVIPNRQSLFWRLMPVGMMVVFLVV
                     GALVTLGIAGFAGGGLALALFGPHLWLLLVLLAFFSWWRQSVVMVPDGCRAVVTRFGK
                     LEEVVEPGRKLLFNPWKQVSYIVNTTREYPYNAPIREAPTASRVNASVDLFLQFRIED
                     PAEFIFTVGGVKGYSDKLENAISEVTRALIYEQRAEDIYDLVGESTQKFLDSLNEQFL
                     PAVRFTNANITHAEPSSQEYRMDLAAPEMVRVAKGAYTYEYELRLRKEQDEGELRSEL
                     ASLRETLSAISAEIATYQAQIDTARERETNRANASARERMVEAESEANANAALLEAQA
                     LDIRAVSSAAAPEILEYRFQQDVLERLATVADRPPQVVQIGATDDDAVDFLEISRRMI
                     GTGGEPLFTPQDMQAIRNRMEEIQARIRERAGELESLAGGQETRLDEEPHQAEEVNAD
                     RVEEIRQSVTDEAVRERVERIGHEGDGEGR"
     gene            25353..26669
                     /locus_tag="VN178_09660"
     CDS             25353..26669
                     /locus_tag="VN178_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWS81279.1"
                     /translation="MMTETMEARFQTQTVARTQEFSRIKEVVSGWSGVRQYLRGGDEG
                     QIVPVVIPKDRRRYGWLFLLGVAIYMLGFALFSGSAALALLAVLVALFFFGLALLWLW
                     RGAIVEIEQGTTGVRSRYGRIEGTLSPGRHYLWRPWDRVEFVVDTATEIPYLAPVAVC
                     PTRENVPLKAIEFFLKFRIVDPLAFVRVIGASNFDLVLSSAVQDAIRQRSRQVSTEKA
                     YDLRGSDVGDMQEYLNRQLSHYGVRILGANIPDVQLPDQYQQHLATRERVAKELAAYE
                     REWELTRKQRIDALLLDIERSKKDRDARLIEVKEARNRARQDVARMLEEQETEAQRVR
                     LEIETRGRAALTEAENEATALRHLGQAHRDNRAVVGYELARRRVEVGEKLMQRAPRPV
                     LIRGEGGEQSALSTLILARLLPQLTADGSTVEKASEALRDPGDGEG"
     gene            complement(26829..27974)
                     /locus_tag="VN178_09665"
     CDS             complement(26829..27974)
                     /locus_tag="VN178_09665"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HWS81280.1"
                     /translation="MSIRTLINPNAREILDGPCPDRTPLRFHRLLPGYVETPLVEAPK
                     LASALGVGKVFVKDESGRLGLPAFKVLGASWAVYRALEDRLGEDFGDWEEIGELRERL
                     EPVRPLSLVAATDGNHGRAVARVARLLGLRAKIFVPDNMALARRKSISDEGAEVIVVD
                     GTYDEAVERSADAAGERALLVSDMSWPGYERIPSWVIEGYSTMLWEIDDELERRNEAG
                     PDLVVVQVGVGAFAAAVARHYRRPEASPPPKLMSVEPASADCLLESVEAGSIVSVPGP
                     HNSIMSGLNCGRPSLVAWPTVSRAIDLLVAVDDGPAREAMRLASESGVVSGETGAAGL
                     GGLLELVGTREEEKTRRALGVGEETRVLLFNCEGATDPDAYGRIVAG"
     gene            complement(28175..29725)
                     /gene="cimA"
                     /locus_tag="VN178_09670"
     CDS             complement(28175..29725)
                     /gene="cimA"
                     /locus_tag="VN178_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HWS81281.1"
                     /translation="MSIKLFDTTLRDGTQREGVSLTAEDKARIARELDSLGIHYIEAG
                     FPASNPKDLEFFEDFDDASLSHAKLVAFTRARRPKGRAEDDPAVTLLPSLSAPVACIV
                     AKSWKLHVEKVLRTTPEENLESVRDTVAYLAGKEVIFDAEHYFNGFRDDPAYALAVLR
                     AAADAGATTLVLCDTNGGTLPTELKAVVARTVREFPDVEVGIHTHNDSGCGVANALVA
                     LEAGATHVQGTINGVGERCGNCDLVTTIANLQLKMGLKVMDEERLRRLTEVSNYVSEL
                     MNLTPDTHRPYVGRSAFAHKGGLHVDGISKDPATYEHVAPEAVGNVRRTPVGELSGKN
                     SIRTKAEELGLEAGDATRILGAIKQREYEGYHYEAADASLALLIGRTTGEDRPLFELE
                     TFRIISEKRADGETTTEATIKLFVGGQRVISTAEGNGPVNALDQALRKAIGPHYPELK
                     EIHLSNYKVRILDEHRATAATTRVLIDSTDGKSMWGAVGVSENIIEASWQALVDGLEF
                     GVHGTKKA"
     gene            complement(29722..30789)
                     /gene="leuB"
                     /locus_tag="VN178_09675"
     CDS             complement(29722..30789)
                     /gene="leuB"
                     /locus_tag="VN178_09675"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWS81282.1"
                     /translation="MRKSFDVLLLPGDGIGPEVVGSAREVIDAAADHFGIEMRYEERR
                     IGGTAIREEGQPVSAQTLEAAGASDSVLLGAVGHPDFDDAPVRPEAGLLKLRKSLGAF
                     ANLRPVSAIPALIGSSPLKREVVDGVDVLIVRELTGGAYFGEKEEGEERASDLSVYTR
                     EEIERVARVAFDAAKRRKRRVTSVDKANVMATSRLWRRVVSEVAEDYPGVSLDHILVD
                     AAAMFLVQAPTRFDVVLTENLFGDILSDEAAMLPGSMGMLPSASLGEPGSPGIFEPVH
                     GSAPDIAGEGKANPYATILSAAMMFRHSLGRPDVAEAIEQGVSVALETHFLTPDVGGK
                     RTTEEVTKAVSRWVAAGEGVA"
     gene            complement(30786..>31118)
                     /locus_tag="VN178_09680"
     CDS             complement(30786..>31118)
                     /locus_tag="VN178_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81283.1"
                     /translation="AASAAVARRVDLPLQEHLSQIEGEPGFTVRGFRGPAGAGDQMSF
                     QNLLLLLGVAALDEFGVTFLRRIFHALWEEEEIVDEERAEELLGEALGPGGREWLVSR
                     PEFQEEQL"
BASE COUNT         5880 a         9674 c         9968 g         5596 t
ORIGIN      
        1 ggaagagatg gtggctgacg gatatcgcca gttcgcggtg cgccacgagg ccggagagct
       61 cgttgccgtg gcgggcgttg ccgtccacgt caacctctac tacgggcgcc acctctacct
      121 ctacgatctt gtggtgacgg agggggcgag gtcaaaagga tacggggagt cgctcctgga
      181 ccatgtggaa ggcatcgccc gccgcgaagg gtgcgagaca gccgcgctgg cgtgcggttt
      241 ggagcgtgag ggggcgctgc gcctctatga gcgcagaggg taccagaagc ccagttacgc
      301 gatgcgcaaa gcattgcgct gagagggcgg tggcgctcac tgatcctata caggagcgct
      361 tcggcgtcct cgcgcgctgg ctcttcctca tcggcttctg gaccgccgcg acaagctcga
      421 tagtcggggc ctggaacggc tcggcttatc tcttcgctga ctccgtgcgc acggctcgcg
      481 acgttccaga cgagagggcc gaagagagta cctctccgag aagagcgttt acttccgggc
      541 gatgctggtc tggatcagct tcccctctat gctcctgctc ttcttcggcg agccggtact
      601 cttgatcatt gtctacgctg ctatgggtgc cttgttcctg cccttcctga ccataacgct
      661 cctctggctc ctcaactccc ggcgcgtggc ccccgaacac cgcaatggca tcgtctcgaa
      721 cataatcctg gtagcctcgg ttttgctctt cgtttttctc gggatacaag aggtactcga
      781 tgccgcctaa atcacccttt gccccatgtt gcttggcagc ccttatccgc aagctcctcg
      841 cacgagcggt tagctcgatc cacagacgcg ctcatctctg acggagaccg cgggtaccgc
      901 tctggaggtg agcccgaagg aggcgctctt tcacctcggg aggcaggtac ttgatcgcat
      961 tcttcaaggc ttcgcgcgag aagcgatcta ggttcgcttc gacgaacccg atgacgcggc
     1021 cctggtccga gcgactgagc tccctcagga cccagcccac acccgtctga gcgaacctct
     1081 cctggctttc taagagccgg gagcagtcgt tgagtatcat ctcagtaaag cctgcgaagt
     1141 tttgatcacc ctttctggcc agattggcga acgcgacgac ggaggcgcgg cgctgccaca
     1201 cgctctgcgt ccctcgccac tccgagatgg cccgtgcgca cggctcgccc tgctgctcta
     1261 ccagcgggcc gagtaccttt acacacaacc agtcgcagat gctccagtcg cggatatacc
     1321 cctcatcaaa aaggcgcgcg aatcgggaca gatcagaacg ccagtccagt gcgccttctg
     1381 acaggaggat ctcctgcagg aacagaacgc ctgcgagctt gtcttccgcg tggtcctcct
     1441 ccaagagcgt gagggccaga tccttctgct gctcaatgga caagagcttt cccagttgct
     1501 cttcctcgaa ccaagtgtgc acagctgccc ggacatcgcc catcttgacg ccgcggaaag
     1561 aggcagtgcc tctgaggtac ttggtccacc attcgcgtgt ggcggccgtt gcgcgggcgt
     1621 tcaaccgtag ctgcagcctg gcgatgaaag cgtcgaatgc tggggcggaa ggtttgcgaa
     1681 ggttcataga tcgttcactg tagctggtcg gagcagcacc cacaggatgt catacatgat
     1741 catgcgatcg tcgtgagcag ggtggccggt cctggctttt tgtgggggta acagaggctt
     1801 cagtctctcc cagtgcttgt cggcccactc gtctcggtgc attcctccag gttatcagct
     1861 ttacggaggc gtcctcgaat gcggtctctc tccgtgaaaa gcaggtgttt attcgctcgg
     1921 atggctgagc gaaacgatag gttaggcgtt ctctcagatt gcgtaggatc aagtgtgctt
     1981 tctctatcct gtctgtgtag gtgcttcttc gcgcggataa agctcccacc gacaggttgg
     2041 tattgcggga ggtggtttcc ctccagctac cgagctcagg acgccgccgc agagcggagg
     2101 gcgaggaacg cggcggacgc cgagagcgcc gcgccgacgg tcgccgcgga gggacgatcg
     2161 ccgccgcgct gctgcaccaa gtctacgagc gtggccaagg cgtcgagcgc ctccatcagg
     2221 gcgttcagcg tcgccatccg gcgcagccac gcacgatcgt cgcgcgcctg ccagagcatc
     2281 acgcccagca cgatctgacg gatccccaac aggcgagcca cgtatcgcgc cgctccagtg
     2341 tcggctcccg gcgtcatgat ggccccgatc gcacgaccgg gcatgatagc cgcgagtccg
     2401 cccgtgacca cgcgttcgat cgctagcgcc ttggccgcta gttcgagacg gtgctccata
     2461 ggcggatcat agcaaaagga agcggggcgg gcggcgctcg ttcctcttct cgcgcctcta
     2521 gcgctgaacc gcaaggccat agctccttcg gaattcacgc gtgccatgaa aggcagttcc
     2581 gttgtcgcaa tcgacgttga ccgaggcgaa tgcggtcacc acaacggccc gctgatgcct
     2641 tccctgcgcg aactgggaat acggcgtgtt ccgcgaaggt cgctggtcgc aggttctcgt
     2701 gagtagcgca catgtttgcc acgctaggca tctcgggtaa agggtgcttc gtcttgatcc
     2761 gaggaagagg aggtccgtct tgagcgagca gcacaccgag caaacgataa aggttaggca
     2821 gatcaccgac gtccacgcca actggagcag ccagggaccg cttgagaacg gcaagttctc
     2881 ttatcagctc atcctggaca atggggccga ggaggccctg atcatgccca ccgcagacga
     2941 tgcgaaggtc ttgcgcgacc ttatccacga tgcggacacc ctctattggg acaccgataa
     3001 ggagatcatc atcttcggca aggtggactg agccgccgat gctctgggct ggggccgtct
     3061 gacggcccca gcctttctct tgccttcgtc tgccagagtg cgtggaacat gaaaactccg
     3121 catttcccat gtggcatctt gcatgtcccc ggttctaggg atgcgggcca gcacgcgaga
     3181 aaacagcgcg cctcaagaga tcccagaggc acagccacaa tccacacgag gagggcagtg
     3241 atgatcctgg ttgacccaat caggcggcac cgatcgggcg agtggtgcca catggtcagc
     3301 gacgagtccg tgggggagct gcacgagttc gccgccacga tcgggctcaa gcgcaagtcg
     3361 ttccagagca cctcgcgccc ccattacgat ctccgtccat cgaagcgccg gctggccgta
     3421 agcatgggag cacaacaggt aggcatgaga gatctcgtga ggagggcggt acggtgaaaa
     3481 ccgaccccct gatcgacagg accggagcgt aggagcctcg gcgcttcttc ttactcatat
     3541 tcagccaagt gcgtggcatg ggtatgtttt gacgttcgcg ccgcgtttgg tcgggtgcga
     3601 ttatgcatac acggtggaaa agatgaccca ctagggaagc aagatggaaa ggagcaccag
     3661 atatgttcgc ggccatacgc tattaccagg cggatcctcc ctcgatcgac gaggtggttc
     3721 ggcgggtgca ggaagagttt gtgcccctta tcagggacat gcgagggttt gtctcatact
     3781 tcatcctggt tcccagcgag agggaggagg atatcgtttc ggtgagcgtc tttgaggacc
     3841 aacaaagcgc cgaggagagc aacaggaagg ctgcagagtg ggtggtgcag aatctcagcg
     3901 agcttcttcg gccaaccccg gagttcgcca gcgggcaagt cgtcgtgtac gaggcttcgc
     3961 agtgaggtag tagcctcacc tcggcggagg aggctgcggg ttcactcttt ggctgaacgc
     4021 cgaagggcac cagcgccgtt gtcgcaatcg gcatcgaccg acacggggtc ggaagcgaca
     4081 acagcccacg ctggcgccgc cctttctcga aagagggtta tcacgaactt cgccttgacg
     4141 gggttctagg aaagtcggcc aaaggaggtg gggctcaccg cgctgctagt gcgtgtctgc
     4201 gaaggaggga tagcggcgct tatcctgctg gcatggcgcg gaaagtgcat gagtcgacag
     4261 acgagcgatg ggcatcggct cgccctagca ttagagactt gtcaatggga ctggaggcag
     4321 acatggagca gacggaacat ggcgatcagc caacctgtgg tgagggactc gcagcaaact
     4381 ccatccttcc ggcgaagctc gcggaactga tggccgcaca ggcggaggtg ctcgagcgcc
     4441 acactagggc cctggatctg gccgacgcga ccgcgcaaaa agaactcgac gcctacgcaa
     4501 agctggagcg tgcccaccgc gacgtggcca gcgaacttgc cgaccttggc gaagcgatgg
     4561 ctggctaccg cgacctaccg atgggtcggc acgacatgac ggtaatgacc gatcccgaag
     4621 ggcagatgga ggcctttcgg cgattcgtcg ccgtggagcg agagctcctg gcgttattgc
     4681 aaacgaaact ggaagcagaa gaagagttgc tacagtagtg gcaaagggcg tgaggtctcg
     4741 tgtgccacac tcgcgcccct ggagttagag aaccacatcc aactcaacct tgccgcttgc
     4801 cccgatcgcc gcgcggaacc ccatgcgcgg cgttgtccca acttgcgtgg gcccacgccc
     4861 acgagatatt attgtgtgcg agcaagggag gcggatctca gtgtaccttc aactactacc
     4921 tcatcaacag cagagatcct gcatcacaag tctcctccgt ctggcacatc ccttccgtgc
     4981 taaggaggga gaagcgccac ggataccatc acacccagaa acccctgcgc ctcctccgtc
     5041 gcgccatttt agcctcgagt agagagggcg atctggtctt cgaccctttc tgtggatcag
     5101 ggaccagtgg ggtagcctcc aaggagctcg atcgcttctt tgtgggaaca gagaaggagg
     5161 aggagtacgc agagcttgca gcccgcagga tagatgctgc ggtgcgaggg gaagtgttgc
     5221 gagagatcga ggaggctcga cccgccgtct gatcttggac cgggtgtcct acgatgtgac
     5281 gtttccagac cgacgccctt ccgtcgcgag ctgacgagct gtcgaagggg atcatagctg
     5341 ttgagtatgg aagcattgca cagttcttaa ctgagcacgg gtatggacct ggagggaaga
     5401 attcgcttga ggcccgcgag tcaatgaagc ggcgtcgtga tgctggctga tggacgctga
     5461 cccgcccgcg ccggcgtccc ctgcgcactg ggagtacgct tgcagtaggt gttgggtgcc
     5521 tagcttggcc gcatgaaccg ccggctccta cgaagccttg cgagcgagtt ccctgtcgat
     5581 ctgatccttg tcgaagccca cgatccagcg gctgccgatc ttgatcaccg gcacacctgt
     5641 ctgcccagtc ttgcgcacga tgtcccgcgc agcatccgcg tcgcgctcta tgttgatctc
     5701 cttgaacggg actccgcgct ccttgaaata acgcttggcc ctgcgacacc aggaacacgt
     5761 tgacgtgctg aatagtagcg ccttggacaa cgaaggccct cctttgcgat gctgccggtt
     5821 gccgtgtgat tataagccgg gaaggcgccg ggagtgatga gcggtagatg tctccattaa
     5881 cagatggtga tctccccctg ccaagcctgg ccgtgaacgg gcctgtggtg gcgtcctgct
     5941 cgtgatccga atggcgaaag tggggcgacc gaataactaa gtggtcgagg tagacgagat
     6001 cgcatcctct agatgctgac cgaagtcgac accgagcggt tagaatggca gatgttatga
     6061 agtcctcaag aggagcgaga tgaccagcgg ctacacccca gggagtatgc ccgggaagga
     6121 ccagcccggc ttcgaccaga tccttagagc cctaagggag gggctcaatg agaactcgcg
     6181 gctcaaagag atgacggccg aagaggtggc taggcaactc gtccagggtg gacacctgga
     6241 agtggagccc cctccacctc tggtcgcgga ggctctggaa ggtctggagg ctgaggagca
     6301 gagtttgcag aacgaggagc tttcctctga ggaggccaac tagctcgtcg tagtgcgcgt
     6361 cgccacgctc aacctgtgga gcgtaggccg gtgctcatcg acgggtttcg cctgctccag
     6421 cccgatctcg ttgcgttcca agaggcggtc gtgagagatg gctacgacca ggtgacggac
     6481 atactcggca ccggccacca tgtcgccggg gtcgtagcgg aactttcggc ctagacctgg
     6541 agcaggcgac cgcttccctg accgctttct cgcccttatg cgccaggacg aactagctga
     6601 gtttcctacc gacggcttcg cgcagtccca cccccgtccg ccgccactcc acccggcggc
     6661 ccaaggtgcg ctccacgtgc gcgaccgcgt cgccttccaa gacgacgagg gtagcgactc
     6721 ccggtccggc tatgtctgcg gcgtcgaagc cccaggcggc ctggggcttg ggccacgccg
     6781 accttgccac cgcccgtagc tcttcccctt ccacctcgcc ggccacggtg gcgtcgggcc
     6841 aggggtacag gcgcaagaca tcggcctcgg tcacgggacc ggcgggcagg tagaagagcc
     6901 caccgtccac aggcggctgc gacgcgagca tgtccacgcc cgcggcccga acgccggtcg
     6961 cttctcgcag agcccgcgcg aagaagtcgg caagcggtgc cgggccgccc gatctggagc
     7021 taaggggttc gaggagctct ccgagcaccc tggtggaagc ctcatcgagg agttcactgc
     7081 cggccccact ccatcttccg cccgtcttgg gcgtcatgca gtaggccctg ggttcttcgc
     7141 ccacctcgag ctcgacgacg ccgagctccg caccgaacgg ccagggctgg actactggag
     7201 ttcctttcac ttggccgatc catctaccca gagtgtggct cgcgaagatc gcatccaccg
     7261 cgtgcgccca cggcctacaa acctccgaga agcgctcggg gttcgcctca tagcctcgtg
     7321 ccccgaagcg ccaatccacc ccgtcgtgca agagcgccac cacccatttc gcgcccgcgt
     7381 cccgaagctc ctcggcgtgc ctgatcgcta cgcctgcgag gtcggggtcg agacttggtg
     7441 cggagacgta gacccccgga tcgggacagg tcagcccgac gaagcctacg gggccggctt
     7501 ccgtttcgac catggctgtg ggcggcaacc caacctcggg ggcgttggcg cacaagagcg
     7561 ggaagcccgt ctccggcgcg tgcgcgcgta ggtgctccat cccccactcg aactcgtggt
     7621 tgccgaccac gccgacgtct atgcctaggt cggctgccgc cgcgaagccc gccttgccat
     7681 tcgagagggt agcgagcgct ccaggtgccg cgaagtcgcc cgcgtccgcc ctgacgatag
     7741 atccctgcgc ccttagcact tcgaccatat ttttcaggcc ctcgccgccc ggcagcgaac
     7801 cgtaggaggt cggcgaggcc gggtaggcgc aacagaagtc gtgagtggcg acgactcgca
     7861 gtggtgctcg ctcgctcatc ggcttacact ccctctatct tcgtgggcaa ctccgtgagt
     7921 gtacggggtt tgctggggct ggctactgcg accttgtgga gagcctcttc acgaacgctt
     7981 caaggtcgac gccacggtcc tggtatctac cggaacctgc ggaagaattg gcggatgtca
     8041 ccgacgagga ggtcgggggc gtcgtgggcg gcccagtggc tgccgcgatc gtactcgctc
     8101 caggagacga tgttggtgtg gtcgcgttcg gcgaacctgc gaaccggccg gaagtcgtag
     8161 gcgaaactcg ccaggccgat gggcgcggtg gtcggcccgg tcgggtgttc ggcatgcttg
     8221 ttctcgtaat agaagcgggc cgctgatgcc gcggtgttgg tcagccagta gatcgagacg
     8281 ttggtcagga tctcgtcagg gctgagcgcg tcaccgaaca gctggccgct ccaggcgagc
     8341 tggccggcgg gcgagtcggc gagagcgtgc gccaggttct gcggcttcga ctcctgtagc
     8401 ctggcgtagg ccgacatttc gtcgttgaag gcctgcagga accgcaattt ctccatgtct
     8461 tcatgagaca gcccttcaaa ctccttcggg tcaccggagg ggaaggagaa gagctggttg
     8521 acgtgcacgc cgattacgtg ctcggggtcg aggcgtccct ggaggggagc aacgatagca
     8581 ccggcgtcgt tgccgtgcgc gccgtagcga tcgtagccga gccgacgcat cagctcggcc
     8641 caagccttcg cggtgcgctg cgcgtcccag cccgcctcgg cggtcggccc ggagaagccg
     8701 aaccccggaa gggacgggat gaccacgtcg aacgcgtcgg cagagtcccc gccgtggacg
     8761 tgcgggtcgg tgagcggacc gatgaggcca aggtactcaa ccaccgtgtt gggccagccg
     8821 tgggtgagga tcagcggcag ggcgtcgggt tcgggggagc ggacgtgcag gaagtggacg
     8881 ttctgcccat caatggtggt ggtgaattgc gggtaggagt tcagcttcgc ttcccaagac
     8941 cgccagtcgt atccggtctg ccagtacccg accaagttct tgacgtagtc tagcgggacg
     9001 ccgtactccc agccaggcgg gacaggaccc gtctggggtc cgctcgcgcc gagtggcagc
     9061 tcgttggccc agcgggtgcg ggacagtcta tcggccaggt cgtcgagatc ggcttggggg
     9121 atgtcgatcc ggaacggccg gatctccgca tccaggcggt cactagtcat ggtcgcaatc
     9181 tcctctcggt gtggttgaca gcggctgtca ggatgtcgtg ggtttaggtg ccgcgcgccg
     9241 gagcgggcag cgtgtgcagg tcgggcgcct tgcacgggcc tcggcacccg actcgccccc
     9301 ttccgacata gcaaactctc cttcctctac ggattccaaa attagatcta tggaacgaat
     9361 gattctgttc cgtagtatag cagaacggaa cggtttgttc cagggtataa tcactaacat
     9421 ggacgcagaa gattcgacgc cgttgagcgg tcggcgcgcc gaggcgcgac gcaacgatcg
     9481 gaagatcctg gaggccgccc gcgcggtgtt cgtcgaagat ccagatgcgc cgatctcgaa
     9541 ggtggccgag cgcgccggcg taggaatcag tgctctttac cgccgctacg cgagcaagga
     9601 ggagcttctc aggcggctct gctccgaggg tctgcggcga tacatcgccg aggctgaggc
     9661 tgcgctggcc gacgaagacg acccttgggt gcccttcgcg gacttcatgt gccgaatcgt
     9721 ggacgccgac acacactccc tgacgctgcg cttggcggga accttcgccc caacggagga
     9781 gctgtaccag gatagtcgga aggcgcagga actcaacttg cggctgttgg agcgaaccaa
     9841 gacggcgggc gccatccgcc acgacatcga ggtggacgac ctttcgctgc tcttcgagca
     9901 agtggccgct gtccgtatcg ggagtgagga gcggacccgc cagcttcgtc gccggtactt
     9961 ggcgctgcaa ttggacgccc ttcgcatccc gtcgggatca ccgctgcccg gccctccacc
    10021 cagctgggag gagatcagtc gacgctggga gaaccagcct tctacgagat cttctgaggc
    10081 acgtggaaag ggttcaccgt tgagcggaag acagtaactc agtacctatc ttggcctcga
    10141 gtagggaggt cgatcttgtc ttcgacccat tctgtggatc tgggaccacg agggttgcct
    10201 ccaaggagct cgatcgcttc tttgtgggag cagagaagga ggaggtgtac gcagagcttg
    10261 cagcccgcag gatgggtgct tcggagcgag agggagtgtt cgagagatcc ccgacctcag
    10321 cgcgagagac gcctgattcc cgggagaacc gcagcaggac ggctttctcg cggtagccag
    10381 gcccaaggtc tcggcgacta cccgcacagg tataatcttg ggtgagttga tcggtgtgct
    10441 gagaagaacg gcggcgcctg cgtatggagt tgcggtcgtt gcggctttcg ttttctgccc
    10501 cgccctcgca gcggcccagg gctcggatac ggtgtacgac gaggccggag cgctcagcgg
    10561 tcccgaagag cagcgcgtgc agcaggcctt cgactccgcg caagaggaga cgggacagcc
    10621 gctctacgcc ttcctggtac cgaacaaggg ggtggaaggt caggaggcgc gccaggaact
    10681 cctgaccaga gaagccaccg aggcgcaagt cccgcaggat gccggggtag tcgtggttgc
    10741 cacgaacgat ggctggggca cgacgtacaa cttcccgcag gacgcataca atacgatggt
    10801 cccggacttt cgggaggggg acttcgccgc cgggctggtg accggagcac gagagattca
    10861 aggtgagcct gcggcacaga acgcgcaaaa cgaacccgat gctggcacgg gcggtctgct
    10921 gggaggcggt ctactccttc tagcagcggt agcaggatct ctcctgttgt tcaggaaccg
    10981 gcgcgcgaag aaacggcgca tggaagaaga acgtcggagt gctgaggaag agttcgccgg
    11041 gctcaccgtc ctcctgaacg agttcgacga gaaggagcgg ctcacctcgg gctacctgga
    11101 ggcccagcgt tcgctgttgg atcagcggac ggaggagtgg atagagacga agatctcgga
    11161 cgccaaagca agcggtttcg cccgggagtt cgatgaggcc gcatcccggc tcacctccga
    11221 ccccatccgg gcacgcgggc gtctggagca tggacgcaac ctcctcgcgg agtccatgca
    11281 gcagcttcac gaagccgaga gaactatgga cgattacagg gccgccgacg aagctctgga
    11341 cggcaggctt catgtggcga aggaggagat acaggcggcc gaagcagcag aggagaccgc
    11401 gcgggccgag ggtgttgccg tcgaaccact gaagctgcgc ccggaatacg accgggtggc
    11461 cagggaggct gcggatcgcg cctcccgccg ggacgagttc gatccccgcc atagcctcgc
    11521 cgcggtcgag acgctcacgg agcgggcccg cgggcacagg aaggccctac agaacgaaat
    11581 ctccgcgcgc gacgcactcc ccgacgaacg ctacgcgacg gaaggagggc ttctgcgggc
    11641 gcgggagacc ctcgaagaat atcgtggagc ccacgagaga gcgctaggcg agttcggcca
    11701 ggccgctctc ggagaggtgc cgaatcccgg cgagctttcc tcgggcctgc tcgaagccca
    11761 agggtgtatg gatcgagcag cccgcgccgc gtcgtcaggc cgcttcggcg aagctcgttc
    11821 cctgctgcag ggagcagccg aaatcgcccg aaagacaacg cagtctccga cccggctgaa
    11881 ggcggccacg gcggaagccg accgtaagaa gcgcgagggc gaggagaagc tccaggagct
    11941 cgaagcccgt ctggcgcagg cgaaagcgaa cgagcacctc atggatcccc gccagcggcg
    12001 gaggctcaga gaatacgagt accaactgga gaacgcccgc tatggtttct tcggggcaga
    12061 ctggctcact gcactgttgc tcttcgaggc cctggacaac gactacatgt acctaggcga
    12121 cccttcatcc ttcggcggcg gtgactgggg cggcggtgac tggggcggtg gggatttcgg
    12181 aggaggagac tggggtgggg gagacttcta accctccacc gtttggcgat gcctggcctc
    12241 ccacatagag cagcgaatgg taggtctttg gcaagccaac tctccttgca tgacgtacac
    12301 gttgtcctcg aggtgcccgt gcgcgagccg aaggcgtagc ctcctgtcca cgggccgaca
    12361 ttcagatggg cgcgacgacg gctgcgccga gatcttcccg cgcagagccg ggaggggtgt
    12421 tccgacagcg gacagtagag atctttccct gcaagcacat cgtccccgag ggtagcgctg
    12481 ttcccctttg cctcgcgcta gaaagcgccg gtatcgatag acggcgcata ggcaggaggt
    12541 ggcttgcgac cacaacatat aggggcttaa catggatgcc tatgaagtcc attgtcgcca
    12601 ttggaggggt tcggtataag gacctcgtga aggctcggac cttgctcgga ccaagagatt
    12661 atgcgtcccc acggagggtg cctggggatc gcgcgatcga tacaacaccc cttcgccgtg
    12721 ccagaaagca tcgtcgccac aggtcccggc tgggcatctt tgcacttgta tttatcttct
    12781 gggtgggctc gtcgtttttg gggttagcag gaaaggggat agcagcgccg atcgagcctt
    12841 cgacattcgc ggctgaatgg ttctggcctt ccaactctgt caccaaggtt tcccctacca
    12901 gggaggatga gggctcgaag ggtaaggaaa ggaaggagca agcgcggaaa cattctggtt
    12961 cggcgacgac ctgcgatgat gtgagagttc tggtagaccg tacgcactcc ctctcccccg
    13021 actacgtgcc cgaagacctc gttgcgctgc aagattacgg ggttcccacc ctgggtagcg
    13081 atgtgctccg gctccgtcgc gcggcggccg agaacctggg ccacctcgtg gaagactccg
    13141 aggcggatgg agaggagcta gtggtggctt cggcctaccg ttcctacgag gagcagcaac
    13201 tctcccacga aaggttgatt agcatcttcg gggcagacgc cggttggatg agtgcgcaac
    13261 ctgggcacag ccagcatcag ctcggcaccg cgatcgattt cactaacgcg gccgcgggct
    13321 accaggtctg gatgcccttc gcacagacga gtgcttacgg gtggctcgag cgccacgcct
    13381 gggagtacgg gtttattctg gcctatccga aaggcaaaga ggagcagacc gggtaccagt
    13441 gggagccctg gcattaccgt tacgttggag tggacgaagc gaaacgactc gaaaagaaaa
    13501 acctgagcct gcaagagttt ctggagcgcg agggaacaat gtcccactgc tgatggccgc
    13561 gttctcgatc gctttcgtgg aagcgaagaa gtttccgaat tggccacgac catgataggt
    13621 aggacgaatt ccgaggggct gggaaaaatc gtcgagagta acatcgatag tgcccgacag
    13681 agcgaccgag tctggtaagc ttccagtgag gggagcatca tgttcgccca gaagatcagt
    13741 aggactctga gtgaggggaa tggaggggat gatgagacga accgttctcg gaatcgcgac
    13801 aacgatggtg gcagcgacgt tcgcggcttg cgggctggcg atgctcgctg aggaggaacc
    13861 ggcccaggcg gcttaccctg gacagaacga ccggatagcg tacgtatctc actcaccgag
    13921 tgggctccac gagatctaca ccatgaaccc cgacggcacg gaccgcaaga agctgaccga
    13981 caatcaggcg tacgatacct cgcctaccta ctcgccaaac ggcaaagaga tcgccttcgt
    14041 gagccagcga gaaggtcaag aaagcggcaa ggtctacacg atgaatgccg aagacggctc
    14101 cggccagaga aacctcagtg gctccacgga tggcagagac cccacctact cgcccaacgg
    14161 tgggaggata gccttcgtgg gccagggcga aaacgaagac atcttggtga tgagcgccag
    14221 ggacggttcc gctcagaaga acctcaccaa caccccgccc gtcaacggtt acgaagtaag
    14281 agagagggac cccgcctact cgccggacgg taccaggata gtcttctaca aggaaacctt
    14341 ccgaccgcat gacgtggacc tctacaagct gaatatgaag cggacgggga cgaagccagc
    14401 tattttgcag ataacagact ttccggggaa ggagtactcc cctgactggt cgcccaacgg
    14461 caacaggata gtcttctacc aagataccag cgccaagggg ttgcgcatct tcaccataag
    14521 acccgatggc acgaatcgac agctggtcgt tcaagatggg aaagatcccg tcttctcacc
    14581 cgacggccgc aagatagcct tcacccggga tggtagcgtc tacacggtga acgcagacgg
    14641 caccgacgag cgccgggtgc ccggtactcc gacggagaac ggcccgagcg aggtcttgcc
    14701 caactgggga cccaagagcc agtgaacgcg gagtagcgtc cttcaggaac ctgagcgcga
    14761 ggcccggggc gtagcagcct cgggcttccc tcttcttgac cccatttacc ctagtttacc
    14821 gtctccccgc ggcggccacc cgattcttgc ccgctctctt ggcccggtag agttcatcgt
    14881 ctgccttctc gacgagctgc tccaggctcg agacgtcttc tgtgagagca gcgacgccta
    14941 cggagaccgt tacgacacta cccaaaagga ttccattctc ggttacgctc tcgtgctcga
    15001 ccccttggcg cacgcgttcg gcgacctcga tggcgtcttc tatattgccg cccatcaaca
    15061 cgacgaactc ctcgcccccg tagcgggaca ccacatcgtc agcccgcgtc gactcacgca
    15121 acacgcgtcc tacacagcgc agggcctcgt ccccgttccc gtgcccgtag cggtcgttgt
    15181 acgacttgaa gtcgtcaaca tcgagcacgg cgcaggcgag gggcagcccg tttctcttgt
    15241 agagttccat tcgccgctcc gccgcccggt gaaagaagga tcgactgacc aggccggtca
    15301 ggtgatccac atcggccgcc ctccgcaagg gggagatttc cactctctcc gcgcggccca
    15361 tctgatgtaa ggccaaaccc acgaaacata cggcgatcag ttcggcgacg tctgcggcgg
    15421 cgtcctctac cgtgctcagt cgagagagaa ccgccaacac ctccgccagt tgtgcggccg
    15481 tgatcaggac cgcggcgacg atcgagatcc gtagtgccag ccggtgggcg gagagcgtca
    15541 gcgcgggcgc cacccgcagc gccagaagga tcacgagccc acccacggcg gcggtaagca
    15601 cgtcgaaggc gatctcgagg atgtgcggta aggctagcgc gttggccata caatccccat
    15661 gttagcaaga gtgctagagc acctcattcc agtcccgaga acggtcaagg tcttgctact
    15721 tcaaattcag gacgattgcc gtatgctcag atcagttgcc agcaacccgg gagggacgag
    15781 agtaggagag cctatgaaac tcaagatcgt attcgagacg ctttgcacag aagagatacc
    15841 gatagcaaac gagtcctgcg gttgggctcc tactctggag gtcgagttct atgtgtaggc
    15901 acatcaagac ccttttcaac ttctcaccac cggcgaccga tgaggagata aatgccgctt
    15961 cgctgcagta cgtgaggaag ataaccgggt tcaacaagcc gtccaagaca aatgaggagg
    16021 ccttctactc cgcggtcgaa gacatcgcga ggatctcgga gcgattgctc gcctcgctcg
    16081 aaacctctgc tccgactaag gaccgggagg aagaggcggc caaggcgaaa gcgcgctcgg
    16141 ccctacgatt cggtgggtga tcacaccgaa cttcgcttca ccaacgaaca catacctgtg
    16201 agcgtgccta gcgccgccaa gatagagcag gcagagagcg gcttctgaag ccaggcaccg
    16261 gagtagaggg tcgaagaaac gtggtcctct actcccgatc gctggctaac cgttgcggga
    16321 gtatctgatc agttccgcac ggcttccgtc gccgtcggtg tgcacgctca acagtgggcc
    16381 gacgttcggg gcgtagaact tcagctcctg gactttaggc tccgtcggta ccaggtcacg
    16441 cgtcatcagg atgttcttgt cgaagtagcc gaagggcacc tggccccgtt cttcaccgac
    16501 ggtgatgacc gcgcccttgt cctcggcttc gttcttgtag aactcctggc ggtaggagag
    16561 gccggggtcc gggttggccg gcatggcgat ccccggttgc gccccgtcga cacccgcctc
    16621 gaacgaacca ccgtggccga tgaccttgcc gttcttgtag ttggtgacgt actcgccgag
    16681 gtaccagatg ttcccagcct tgtcctaggc gtaccagtcc tcggtgatct cgaccggcga
    16741 gccgttctcg gttaccgtgt cgcgtatcac gcgggcctcg atcccgttgg ctatcatctt
    16801 cgtcttgtcg gtgacctcga cgacgacctt ctggttggtg cccttactgt ccgtctctct
    16861 gtagacccac ttgcttcccg ggctcatcgg ccagtaggga ttgtcgatgc tgaccgagaa
    16921 gtcggccggg tccagcttta cgggctcgct tccctgcggc atgcctcccc ggccgccaga
    16981 tcccgaagcc tcgccggtcg tgctccctgg actctcgccc ttctctgccg tcgtactggt
    17041 cccacatgcg ccaaccaatg ccagaccaac cgccagcgcg agtgccacca caccgcgcat
    17101 caggggtctc gtacgcttcc cttccatcgt tcctcctctt cgatcgccgc cgttcgcgca
    17161 cggcgccctt tacagccctg cctcggtccc cctttcagcc tatttcacag accttcgtgt
    17221 acgtgaagcc tgatagcagc attgcaggcg aatgtgagga cacgttgagc gcgcggtgag
    17281 agcaggatta aactattcgg aaaccgtccg tactcttcgc cctttcggac gtcactggag
    17341 cttcgatcga catcctcccc aagcgggtga tattcccagc ttttggcgat tatcgaagct
    17401 cgatctgacc tctacttctg gtcagagatc acgacatctc gtgccggtgg cgggtggcgc
    17461 ttaccccatc tcgtgggctg tcgcaagctt cccgtggacc gagtatcatc cttatctgga
    17521 aggcgtacga caaggtgcca cgcgtggggt tgctagaagg gattagacat tgacgcgtcg
    17581 gtacatggca acaggagcgg ctgcagattc ggtccggcct cccgccaacg gcgccattta
    17641 tggctctggg cgcgctcggc ggcgacgcgc gttcgggggt agagcgcgtg agcgaggcgg
    17701 tgtatagggc tatgttcgag gccgttgttg tcactatgac ccgcgttccc cacgtcctcg
    17761 cgatcatggc cgcagtcgtt ctcttctacc tggcttaccg acgtcgaccg gggccgccga
    17821 agtgggggat gtggcccggc atcttcgcgg tgctcgcgct ctatggattc ctggccgcgt
    17881 tggcacccgc cggatgggac gagccgctcg tgctgctggg gataggcttc gccgccttgt
    17941 ctggatatgc ctggctggat cccgacagaa ggaggcattc ggcgggtgcc gggcaaacct
    18001 ccaggaggcg cgggtggtga cttcccctgg tccccccgtg gagcctgttt cacgggtgag
    18061 cgtacggtgg gcgggtagaa cccagatcta taatcggcgt tgatgatggc gctgccagaa
    18121 cactcgccgg ggacgctccg cacaccggtg atgatggctt tgatcgccgt catgatctcc
    18181 ctgtttccgc tacttttcgg ctgttcctct gaagggccca aagaccagtc cgacggtagc
    18241 cgacgaaccg acgagaaccg gccggccggc gcgacgcccg gaggacaaac caccggcgac
    18301 gggtcgaagg gcgcccgccg aaccgaaaca caggcggccc tcgtccccat tgcccacctg
    18361 agctcgaccg cggagaacat cagcacgcag gagctttcgg agacacgaga tctggccgtc
    18421 ggccgggagt accgtgatga ggccgaagag ctcgtagata gcccgggatt cgaaagcttc
    18481 gattctgggg ccgccgtggt cgaccacgta agcaagacac ccgatgcgct cgggctcgta
    18541 ccctggggcg aggttggccc aagggtcaaa gcactgtcgg ttgacggtag gtcgctactg
    18601 gactcggagg gtggcggaga ctattcactc gcgccggagg gtgcgacggt ccctgatccc
    18661 caagagctgc ggcgcgttgt ggtagggggc gacatagtgt tcgaccgcgg gcagtactac
    18721 atggtcatcc agagggggat ggggatcgac ttcccgctcg gcgggggcta cgccgccgta
    18781 acgggtcgcg tgccggagca gagcatctac tcggagacgg ggatcatcca ccagttcacg
    18841 gctaggcgta ccgggagggc gggcgccgtc agggagtatc tgtccggcgc cgaccttacg
    18901 ctcgccaact tcgagaaccc ggtcataagg gactccgtct atcatcccga cgcaacgacc
    18961 ttcacgggcg acctgcggtt gatgccggta ctggatcagg ccggcatgga cggcgttacc
    19021 ctgggcaaca accacattct ggatgcgggg actacggggc tcgacgagac gatgcggcac
    19081 ttggatgacg ccggaatagc ccacgccggg gcgggtatgg accttgcgga ggcccgcaag
    19141 cccatggtgt tcgatctcgg taagatgaag atcggcgtcc tctcatacct ggacgtcccc
    19201 tcctacggct gggcctgggc tacccagacg gcccccggca cggcgccact ccttcagaac
    19261 gtcataaagg aggacataga gcgcttgcgc ccgagagtag acctgatcct cgttatgcca
    19321 cattggggca aggagtacat cgccactccg gagccgcaac aggtggatct cgcgcacgcc
    19381 gccatagacg ccggggccga cctcgtagtc ggcggccacg cccactggcc caaggggatc
    19441 gaattgtacg aagggaagcc gatcttctac ggcgtcggca acttcctcct cgaccaatcc
    19501 tggtcggaag agacctcgac gggcatcttc gcggagatta cattgcacgg ggacgatgtg
    19561 gcccaggtcc gacccgtccc cttcatcatg ctcgaccgcg cccagcccaa cttcctcatg
    19621 ccaggggctg gcggggatag gacattgcgc aagatcttcg ccgcatccat cggtcccgag
    19681 ttcgagacct acaggcgccg cgcccgctga cgttctcgag gtcctcgacc agaaagccca
    19741 tacgttctgg gcgtccgctt tagccggatc tccctgcccg accgcgagaa tctccatgtc
    19801 gatctctgcc tttcatcgca ggcggcgtga aatcttgggc gatgttacgc tgggttcgtc
    19861 gagttcggag cggatgctgg gttgcgggaa ggatgggtga catgcacgag caagaaaatc
    19921 tcaagacgat acatcaggtc tacgcggctt tcggtgaggg cgacatcgag ggtgtgcttg
    19981 gcatgctgac ggacgatgtg cggtggagta cccctggtcc ccccgatgtc atcccctacg
    20041 ccggtttgag gaccggccac gaccaggtag ccgggtactt caagtcgttc ggagaggcag
    20101 tagagaccac agcgttcgaa ccacagaggt tctttgccca ggatgacatg gtcgtggtgc
    20161 ttggacggta cgccttgcgc gtcgtgagca cgggcaacgc tatcgacaac gattgggttc
    20221 acaccttcaa actggccgaa ggcaagatct cggctttcga gggttacgaa gacagcgccg
    20281 ccgtggtcgc ggcgttcacc gcgaaaccgc actaagggct ctgatcgacc actactcttg
    20341 cagtcgtggg acgtgaggtg tccctgcaaa gacgaggttc caagtacctt cacaagacga
    20401 atttcgtaac gcttgccgta accttcggga gacgggtgtg tcgctcgccg tcgtcatcgc
    20461 cgacacgcac ctaccgcggc gcgccagcgc attaccggag gtcctgatac cccaactgaa
    20521 gcgggccgat ctgatcctgc acgcggggga cctcatggta cctaagctcc tggaggactt
    20581 agccgcctac gcccccgtcc gggcggtgcg gggtaacctc gatcctcctg acgtacggct
    20641 gccggagacg gtgcagtttg agttcggcgg tgtgagcatc gccatgatcc acgactcggg
    20701 ccggaagaag ggacgccgca gcaggatgag acggcgcttc cctgaggcgc gggtagtcgt
    20761 cttcgggcac tcgcacatcc cctggctaga ggacgcggaa ggactcctgc tgctcaaccc
    20821 tggcagtccg acggacaggc gtcggcaacc cgagcatacc ttcgcgctac tctgcgtgga
    20881 gggcggcgag gtgcgtgcgg aggtattggc gttgtgaggc cgccgacgca gggaggcaag
    20941 ccgcgctcga ctataatttt gtctgtggcg gatgacgagc acaggagatc cgaaaacagg
    21001 gtcttcctcg aaaggagatc ctacgaaccg cagctggcgc tgcggcgggt ggagagcgcc
    21061 gtactggctc tcgtaatcat taccgttgta ggcattctcg ggtacatggt cttagaagga
    21121 tggagcttta ccgactctct ttacatgacc gtgatcacgc ttaccacggt cggatacaag
    21181 gaagttcgcg tgctcgatac atcagggcag ctgtggacca tgctcctcct gataacgggg
    21241 gtgggcacgc tcttctacgc tgcagtatcc tccgtcgaac tggtggtcga aggaacggtg
    21301 cggggctacg ttgggaggag aagggtgaag gcggcgatcg gcaggctaaa cggccactac
    21361 atactgtgcg gctacggcag ggtcggaaaa caggtcgcgc gtgaattcgt ggctgatgat
    21421 gtgcctttcg tagtcgtgga acaggatcag gatatcctgg aggagtgcct ggccgaggga
    21481 aatcttgcgt tgctcggcga ggcatcggac gacgaagtcc tcgaggaggc cggcatccta
    21541 cgcgcgggag gactcgtcgc ggccgtcgac tcagacgcgg acaacgtctt cgtggtgctc
    21601 tcggcccgaa agctcaaccc gaagctgcac atcgtcgccc gcgcctcctc cgaggaattg
    21661 gcggacaagc tggagatagc gggggccgac cgtacgctct caccctacgc cgtgggtggc
    21721 aggcggctcg cctcactggc tacgcagccc ctcgtcgtcg actttctgga cgtcgtcacg
    21781 cgcggcgaga agggcatcga gttccacctg gaggagtact tcgtgcccga agattcgacg
    21841 atcgccgggc acaccatcgg ggagctcgag ataggggaga gaaccggcgc catagtcctc
    21901 gccatcagca ccacggaagg caccttcgac accacgccat cggcagatgt tcgtctgcgc
    21961 gccggcgaca ccctcatcgt cctggggagc cgcggccaga cagaacgcct ggagcacctc
    22021 atgcgcggta cgtaggggcg ggttccaaac ccgcccgttc accaaacccg cccctcattc
    22081 cctcggccgg tagatcacca tgtccgctac acgatccgtt ctctggtaac tatcgtgtat
    22141 ctcttcgagc atccttgggg tccacctgtc acgctttatc tctcttgcga aggatgggct
    22201 ccagatcatt atcatggaaa acttctgctg gtcgatgtcc ttgaggatcg gcctctggtc
    22261 ccagtcacca tcgagggtga gctgggtcat ctcgaaaggc tggaagtaga tcctccgccc
    22321 ctgctgcggc aggagtccca tgtagtcgtc cgtgagcacg ggcccatccg cgcccgcaac
    22381 catacgggat agttgcgcga cctcgccccg ctgctcgata acgtagtcct gcaaacccga
    22441 agcgacccga gacgattgtg cgagcgccag tacctggatc gcgagaaggg ctataagggc
    22501 gatccgtaat cgcggccgcc tgcgctgcca cgcgataaag gccccggtcg ccaggcccag
    22561 cgccgcggac aactccaaca gatagttcac atctgaaccc accttgccca ccagcaccgc
    22621 ggagggaatg ctggcgagca gataaggtgc gacgagccac caggaacggt tcccctcgcg
    22681 cggcgtgagc accaagaacg cgagcgagcc gagcagtagc aacgggcagg caaggagggc
    22741 gccgagggcg ttgaggctaa cccgctccca gcggaagtca ttgaggttgg cggttacggt
    22801 gttgaggaag aacccgcctc cggtaaagat gttcagtagc gcgaacacga tcaggcctgc
    22861 tcctccggct atagcggcag tctccagcgc ccggcggcgc tggccctggg ccgcgagcca
    22921 cacgaaggcc gcaagcggtg cggccaggat gtaggtctgg cgggtataga cggccgcgac
    22981 gagaagcagg gccgagacga agacgctcca gcgcttctcg ggccagcgcg cgacgacgaa
    23041 cagcccggcc caggagagcg ccagtcccag cgagtcaacc ctggagagcg aagaccagcg
    23101 caccacgtag ggtatggcgg ggaagatcag accgcccgct atcgcggcga tcctgtcttt
    23161 ggtcagcgcg tgcaaggtca gccccacaaa gagcgccaca gccacgacgc tgaggagcga
    23221 gatcagacgc ccgtaccaca gcgcgggccc gaacagccac accaacggca cctgggtgag
    23281 catgaacagc ggaggataat tggagatgac gtagggcgag acggcgaggt cggtcctgta
    23341 gatgttctga aactgagcga gccttacgga ctggtcgagc agcggacctt cgccgtagtt
    23401 gagcgggtaa gggaaagcga gcgcgtccca tccgtgccgc aaaaatagca gcgcggcgag
    23461 accttgggca atgagcagca tcgcggtggc ggcgaagagt gcgacccggc gcgtccgccc
    23521 gcccgtgtgg gctgctttct ctaaaccctt actcgtcgcc aggcccttcc gtgtacgcga
    23581 actcaggccc tgtatcctac cgcttttggc gccggctacg cgcgaacgat gccagtcgct
    23641 ctgtgccggg cgttccgaag gaccccactc caggttgtgc ggccaaccgc accgtgtact
    23701 atggaggtat atcgccctaa cattcaggag ggttttctga tggagacgct cggcgagcag
    23761 gcgcgggatc ttacggaagg catcgctcgg gaggcgtccg agcagttcag gatcgacgcc
    23821 agggactttc tctcggcgcg ggagcgtggc gggaccacgg ggactcgaat agaccagcag
    23881 acggccgccc tctccgacgc cgccgagatc gtcaatcgca gtttccctac gctggagccc
    23941 tcggggaggt tcataaacgc gatctctccg gtcgtcatcc cgaaccgcca gtcgctgttc
    24001 tggcgcctca tgccggtagg gatgatggtc gtattcctgg tcgtcggcgc gctggtgacg
    24061 ctaggtatcg caggcttcgc cggcggcggg ctggcgctgg cgctcttcgg gccgcacctg
    24121 tggctgcttc tagtcctgct ggccttcttc tcctggtggc gccagagcgt ggtgatggtc
    24181 cccgacggct gcagggccgt cgtcacccgg ttcgggaagc tggaggaggt cgtcgaaccg
    24241 ggacgcaagc tgctcttcaa cccgtggaag caggtcagct acatcgtcaa caccacgagg
    24301 gagtaccctt acaacgcccc cataagggag gctcctacgg cgagccgggt caacgcgagc
    24361 gtcgatctgt ttctgcagtt caggatcgag gaccccgccg agttcatctt cacggtaggc
    24421 ggggtcaagg ggtactcgga caagctggag aacgcgatca gcgaggtcac gcgggccctc
    24481 atctacgagc agcgggccga ggacatctac gacctcgtcg gggagagcac ccagaagttt
    24541 ctcgactcct tgaatgagca gttcctcccc gccgtgcgct tcacgaacgc gaacataacg
    24601 cacgccgagc cctcgagcca ggagtacagg atggacctcg cggcccccga gatggtcagg
    24661 gtggcgaagg gggcttatac ctacgaatac gagctcaggc tccgaaagga gcaggacgag
    24721 ggcgagctca ggagcgagct ggcgtcgctc agggagacgc tctccgcgat cagcgccgag
    24781 atagcgacct accaggccca gatcgacacc gcccgcgagc gcgagacgaa ccgcgccaac
    24841 gccagcgccc gcgagaggat ggtcgaggcc gagagcgagg cgaacgcgaa cgccgcgctg
    24901 ctcgaggccc aggcgctgga catcagggcc gtcagcagcg ccgccgcgcc ggagatcctg
    24961 gagtatcgct tccagcagga cgtactcgag aggctcgcca cggtagccga caggccgccg
    25021 caggtggtgc agatcggggc cacggacgac gacgcggtgg acttcctgga gatctcgcgg
    25081 cgtatgatcg ggaccggagg agagcctctt ttcacgcccc aagacatgca ggctataagg
    25141 aacaggatgg aggagataca ggcccgcatc cgcgagcgcg ccggcgagct cgaaagcctc
    25201 gccggcggcc aggagacgcg cctggacgag gagccgcacc aggccgaaga agtgaacgca
    25261 gacagggtcg aggagatccg gcagtccgta acggacgagg ccgtgcgcga gcgcgtagag
    25321 cggatcggcc acgaggggga cggtgagggg cgatgatgac cgagaccatg gaggcgcggt
    25381 ttcagacgca gaccgtcgcc aggacgcagg agttttccag gatcaaagag gtcgtctcgg
    25441 ggtggtccgg ggtaaggcaa tacctgaggg gcggggacga gggtcagatc gtccccgtcg
    25501 tcataccgaa ggaccgcagg cgctacggct ggctgttctt gctcggtgtg gcgatctaca
    25561 tgctaggctt cgcgctcttc tcggggagcg cggcgctcgc cttgttggcc gtgcttgtcg
    25621 cgctcttctt cttcggcctc gcgctgctct ggctgtggcg gggcgcgatc gtcgagatcg
    25681 agcagggtac taccggcgtg cgctcgcgct acgggaggat cgaaggcacc ctctcgcctg
    25741 gcaggcacta cctctggcgg ccgtgggaca gggtcgagtt cgtcgtcgac acggcgacgg
    25801 agatccctta cctcgcgcct gtggccgtct gcccgacgcg ggagaacgtg ccgctcaagg
    25861 ctatagagtt cttcctgaag ttcaggatag tggacccttt ggccttcgtg cgcgtcatcg
    25921 gcgcctcgaa cttcgacctc gtcctctcca gcgcggtgca ggatgcgatc cggcagcgca
    25981 gccgccaggt gagcacggag aaggcctacg acctgcgcgg cagcgacgtc ggggatatgc
    26041 aagagtacct gaaccgccag ctctcccact acggggtccg catcctcgga gccaacatcc
    26101 ccgacgtgca gctccccgac cagtaccagc agcacctggc cacccgcgag cgcgtagcca
    26161 aagagctcgc cgcctacgag cgcgagtggg agctgacccg caagcagcgc atagacgcgc
    26221 tcctcctgga tatcgaacgc tccaagaaag atcgcgacgc ccgcctcata gaagtgaagg
    26281 aagcccgcaa ccgcgcccgc caggacgtcg cgcgcatgct cgaagagcag gagaccgagg
    26341 cccagcgcgt gcgcctggag atagagactc gcggccgcgc cgccctcacc gaggcggaga
    26401 acgaggccac ggccctccgc cacctcggcc aggcccacag ggacaaccgc gccgtggtcg
    26461 gctacgagct cgcccgccgc agggtcgaag tcggcgagaa gctcatgcaa cgcgcccctc
    26521 gacccgtcct catccgcggc gaagggggcg agcaatctgc gctctccacc ctcatcctcg
    26581 cccggctcct cccgcaactg accgcggacg gctcgacagt ggagaaagcc agcgaggcac
    26641 tgcgcgaccc tggcgacggc gaggggtagt ctcagagcat aaccataatt ctcgcctccc
    26701 cgctcgtcat tcccgcgaag cttgtccccg cgaaagcggg gagcgggaat ccactacggc
    26761 gatcgctcgg agcgatcctg gcggtgctct ccagtacccc gaggctggaa cagggcaagg
    26821 ttgtccggct accccgccac tatcctcccg taggcgtcgg ggtctgtggc gccctcacag
    26881 ttgaagagca ggacgcgggt ctcctcaccc acgcctaaag ccctacgcgt cttctcttcc
    26941 tcccttgtac ccaccagttc cagtagaccg cctagtccgg cagccccggt ctctcctgac
    27001 accacgcctg attcggacgc gaggcgcatg gcctcgcggg ctgggccgtc gtcgacggct
    27061 acgaggaggt cgatggccct ggatacggtc ggccaggcta ccagtgaggg tctgccgcaa
    27121 ttcaggcccg acatgatgga attgtgcggc cctggcactg acacgatgct ccctgcctcc
    27181 accgactcga gcaggcaatc cgcgctcgcc ggctcgacgc tcatgagctt cgggggcggc
    27241 gaagcttctg gacggcggta gtggcgggcc acggcggcgg cgaaggcgcc gacaccgacc
    27301 tgcacgacca ccaggtcggg tcccgcttca ttgcgcctct ccagctcgtc gtcgatctcc
    27361 cagagcatcg tcgaatagcc ttcgatcacc caggaaggta tacgctcgta gccgggccag
    27421 gacatgtccg agaccaggag cgctcgttct cccgctgcgt cggccgagcg ctcgacggcc
    27481 tcgtcgtagg ttccatctac cacgatcacc tcggcgcctt cgtcagagat ggacttgcgg
    27541 cgcgccagtg ccatgttgtc tggcacgaag atcttcgctc ggagccccag tagccgcgcc
    27601 acccgtgcca cggcgcgccc gtggttgccg tcggtggccg cgacgagcga gagcggccgc
    27661 acgggttcga gacgctccct gagctccccg atctcctccc aatccccgaa atcttcccca
    27721 aggcgatcct ccagggccct gtatacggcc cacgatgcgc cgaggacctt gaaggcgggc
    27781 aatccgagcc ggcccgattc gtcctttacg aagaccttcc cgacgccgag ggcggaggcg
    27841 agcttcggag cctcaaccag tggcgtctcc acgtagccag gcagcaggcg gtggaatcgc
    27901 aggggtgtac gatccgggca agggccatcg agtatctccc tggcgttcgg gttgatgaga
    27961 gtcctgatac tcacgtcctg ttcgaatgct gcctggagtc cgggcgtggg agcatacgta
    28021 atccggaata tcgcgtccgc ttggaggagc agatcctaca gtgcggtgga ttcccgcttg
    28081 cgcgggaatg acggcattgc cttgagtgaa ggactagtag tcgtgctcgc caaggaggtc
    28141 gtacgccgta ggaagtaatc atgcggattc cgtatcacgc cttcttcgta ccgtgcaccc
    28201 cgaattcgag gccatcaacc agggcttgcc aggaggcttc gatgatgttc tcggagaccc
    28261 ctactgcgcc ccacatactc ttgccgtccg tcgaatctat gaggacgcgg gtcgttgcgg
    28321 ccgtggcgcg gtgctcgtcg aggatgcgga ccttgtagtt ggagaggtgt atctctttca
    28381 gttccggata gtgcggccct atggcctttc tcagcgcctg atcgagggcg tttacgggac
    28441 cattgccctc cgctgtagag atgacccttt ggccgcctac gaagagcttt atagtcgcct
    28501 cggtggtcgt ctcgccgtcg gcccgcttct cggagatgat cctgaacgtc tccaactcga
    28561 agagcggcct gtcttccccg gtggtccgcc ctataagcag cgcgagggag gcgtcggcgg
    28621 cctcgtaatg gtatccctcg tactcacgct gctttatggc gccgaggatc cgggtcgcat
    28681 ccccggcctc gagcccgagc tcctcggcct tggtcctgat ggagttcttc cccgagagct
    28741 ctccgacggg cgtccgcctc acgttcccta ctgcctctgg cgcgacgtgc tcgtaggtcg
    28801 cagggtcctt ggagataccg tcgacgtgca gcccgccctt gtgggcgaag gcgctacgcc
    28861 ccacgtaggg acggtgcgtg tcaggcgtca ggttcataag ctccgagacg tagttggaga
    28921 cctcggtgag ccgccgcaac ctctcctcat ccatgacctt caggcccatc ttgagctgca
    28981 agttggcgat ggtcgtgacg aggtcgcagt tgccgcaccg ttcaccgacg ccgttgatgg
    29041 tcccctgtac gtgcgtcgcc ccggcctcta gagccaccag ggcattcgcc accccacacc
    29101 ccgagtcgtt gtgggtgtgg atgcctacct ccacgtcggg gaactcccgc accgtgcgtg
    29161 ccaccaccgc cttgagctcc gtgggcaggg tccccccgtt cgtgtcacag agaaccagcg
    29221 tcgtcgcccc tgcatcggcc gctgcccgca gcaccgcgag cgcgtacgcg ggatcgtcgc
    29281 gaaagccgtt gaagtagtgc tcggcgtcga agatgacctc tttaccggcc aggtaggcga
    29341 cggtgtcgcg cacggactcc aggttctcct ccggcgtggt cctgaggacc ttctccacgt
    29401 gcagcttcca actcttggcg acgatgcagg cgacgggcgc cgagagggat ggtaggagcg
    29461 ttacggcggg gtcgtcctcg gccctgcctt tcgggcgccg ggcccgcgtg aaggcgacga
    29521 gcttcgcgtg ggagagcgaa gcgtcatcga agtcctcgaa gaactccagg tccttgggat
    29581 tggaggcggg gaagcccgct tcgatgtagt ggatccccaa agagtccagc tcgcgggcta
    29641 tgcgggcctt gtcctcggcg gtcaggctga cgccttctcg ctgggtacca tcacgtagcg
    29701 tcgtatcgaa gagctttatg ctcatgccac accctctccc gccgcgaccc acctgctcac
    29761 ggctttcgtc acttcttcgg tagttctctt tccccctacg tcgggggtca aaaaatgggt
    29821 ctccaaggcg acggagacgc cctgctctat agcctcggcc acgtcgggac ggcccaggga
    29881 gtggcggaac atcatcgccg cggagaggat ggtcgcgtag gggttcgcct tcccctctcc
    29941 cgcgatgtcg ggggccgagc cgtggacggg ctcgaagatg ccaggggagc ccggctcgcc
    30001 cagagaagcg ctgggaagca tacccatcga gcctgggagc atcgctgcct catcggagag
    30061 gatgtccccg aagaggttct cggtaaggac gacgtcgaac ctcgtcgggg cctgaaccag
    30121 gaacatcgcc gccgcgtcga cgaggatgtg gtcgagggag accccaggat aatcttcggc
    30181 cacctcagag acgaccctgc gccacagccg gctcgtcgcc atgacgttcg ccttgtccac
    30241 gctagtcacc cgccgcttgc ggcgcttcgc cgcatcgaaa gccacccgcg cgacccgctc
    30301 tatctcctcc cgcgtgtaca cgctgaggtc gctggcccgc tcttcaccct cctccttctc
    30361 cccgaagtaa gccccgcctg tcagctccct gacgataagc acgtcgacgc catcgacgac
    30421 ctcgcgtttg aggggtgagg agccgatgag cgccgggatg gcggacacag gcctcaggtt
    30481 ggcgaaggcc cccaggctct tcctgagctt cagcagtcct gcctcaggac gcaccggggc
    30541 gtcgtcgaag tcggggtgcc cgaccgcgcc cagaaggacc gagtcggagg cgccggcggc
    30601 ttccagcgtc tgtgcggaga cgggctggcc ttcctcgcgg atggccgtgc cgccgatcct
    30661 gcgctcctcg tagcgcatct cgatgccgaa gtgatccgcc gcagcgtcga tgacctcgcg
    30721 cgctgacccg accacctcgg gtccgatgcc gtcgccgggg agaagtagta cgtcgaaaga
    30781 ttttctcata actgctcctc ctgaaactct ggcctcgaca ccagccactc ccttccgccc
    30841 ggacccagag cctctcctag cagctcctcg gcccgttcct catcgactat ctcctcttcc
    30901 tcccacagtg cgtggaagat gcgtcgtagg aaggtcactc cgaactcatc cagcgccgcc
    30961 acaccgagca gtaagagcag gttctggaac gacatctgat ccccggcccc cgccggtcct
    31021 cggaagcccc gcacggtgaa cccaggctca ccctctatct gcgacagatg ctcctgcagg
    31081 ggcagatcga ccctgcgggc gaccgccgcc gaggcggc
//
LOCUS       DATMVF010000194         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_123483, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000194 DATMVF010000000
VERSION     DATMVF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123483"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(165..282)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(430..1365)
                     /locus_tag="VN178_09685"
     CDS             complement(430..1365)
                     /locus_tag="VN178_09685"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWS81284.1"
                     /translation="MKNMLSSSILDAIGNTPMVEIEGIYCKLEYLNPSGSIKARIAKY
                     MIERAEDEGLLKRGDTIVEATSGNTGNALSMVAAVKGYRMLVVMPEGMSGERVAISRA
                     FGAEVMEVGHFHVNEALAKAEELGQEPGYFCPAQFESEWNVEENRIWLGPELLAQLPE
                     NVVPDALVMGVGTGGTLIGVGQAFREVNPDVKLFAMEPSESSTILCGEVGDHLIEGIS
                     DGFVPGIFERHKKLVDTALTVDSEEAVEEMRRLARQYGLFVGPSSGANLIAVRKVRDA
                     YPELETIVTLFCDEGEKYIQDHFAEKVEVTPETFA"
     gene            complement(1378..>2047)
                     /locus_tag="VN178_09690"
     CDS             complement(1378..>2047)
                     /locus_tag="VN178_09690"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HWS81285.1"
                     /translation="VCIADLADEPGQEAASKIGGIYVHADVTDPDDVQRMYSETAERF
                     GGIDVLFNNAGISPPDDDSILDTDLEAWERVQNVNLKSVYLCCKYGIPYLLERGGGSI
                     INTASFVAVMGAATSQISYTASKGGVLALSRELGVEFARRGIRVNALCPGPVNTPLLQ
                     EIFAKDPEKAERRLVHLPMGRFAEATEIAGAVLFLASDDASYVNASTFLVDGGLSGAY
                     VTPE"
BASE COUNT          340 a          656 c          625 g          426 t
ORIGIN      
        1 tggcctccag catctcggct atgtcggact cggtgtgcgg tgtgaatcgc gccacgcggt
       61 gctaccccct gttctcgggt ggacgttccc tttttccgtc ctgtcccaaa acctgagagt
      121 gttaccccgt cggcgccggt ccgtgccgga ctctccagga cgccctccgg ctcgcggtcc
      181 ttttgcctga gagattcagg ccgctctcgc aaccctttca ccttcggcgg ccgtcacagg
      241 atctccccca tcccccgaac gactctctcc cgcggccctc atcgggctag ttcggactat
      301 agcacaaggg tggcgcgcta cccgtagacg gcgtcgaggc agaggcgctt gactagggcg
      361 cggcggcctc ggacgctgag cttctggaag tcgtgactcg ctaccggacc aggctaccgg
      421 tggccctgct caagcgaagg tctccggggt cacctctacc ttttcggcga agtgatcctg
      481 gatgtacttt tcaccttcgt cgcaaaatag ggtgacaata gtctctagtt ccggataagc
      541 atcgcgcacc ttgcggacgg caatgaggtt cgcgccgctg gaagggccta cgaagagacc
      601 gtactgcctg gccagacggc gcatctcctc tacggcctcc tcggagtcga cggtcagggc
      661 ggtatcgacc agcttcttgt gacgctcgaa gatgccggga acgaagccgt ccgagatgcc
      721 ctctatgagg tggtccccca cctcgccgca caggatggtc gaggactccg agggttccat
      781 ggcgaagagc ttgacgtcag gatttacttc tctgaaggcc tgacctaccc cgatgagggt
      841 gcctccggtg cctaccccca tgacgagggc gtcggggacg acgttttcag gcagctgggc
      901 cagtaactcg ggtcccagcc agatcctgtt ctcctccacg ttccactcgc tctcgaactg
      961 ggccgggcag aagtagccgg gctcctggcc gagctcctct gccttcgcca gggcttcgtt
     1021 gacgtggaag tgtccgacct ccatcacctc ggccccgaag gcccggctga tggcgacccg
     1081 ctcgccgctc ataccttcgg gcatcaccac gagcatcctg tagcccttga ccgcggcgac
     1141 catagagagg gcgttgccgg tgttgcctga cgtggcctcg acgatggtat cgcccctttt
     1201 cagaagcccc tcgtcttcgg cgcgttcgat catgtacttc gcgatcctgg ctttgatgga
     1261 accggaaggg ttcaggtact cgagcttgca gtagatgccc tctatttcca ccatgggcgt
     1321 gtttcctatg gcgtcgagga tgctgctgga aagcatattt ttcatgccga ccacctccta
     1381 ttcaggggta acgtatgccc ctgagaggcc gccgtcaacc aggaaggtcg aggcgttgac
     1441 gtaggaagcg tcgtcggagg ccaggaagag gacggcgccc gcgatctctg tcgcctcagc
     1501 gaaccggccc atcggcaggt gtacgagcct gcgttccgct ttctccgggt ccttggcaaa
     1561 gatctcctgc agaagcggcg tattcaccgg gccggggcag agcgcgttga cgcgtatacc
     1621 ccttcgggcg aactcgaccc ccagctcgcg gctgagggcc agcacgccgc ccttcgatgc
     1681 ggtgtaagag atttgggagg tcgccgcacc catcacggcg acgaaagagg cggtgttgat
     1741 gatcgatcct ccgccccgct ccagtaggta aggtatccca tacttgcagc acaggtagac
     1801 gctcttgagg ttcacgttct ggacgcgctc ccaggcttcg aggtcggtgt ccaggatgga
     1861 gtcgtcgtcg ggcggagaga tgccggcgtt gttgaagagc acgtctatgc cgccgaaacg
     1921 ctcggccgtc tcgctgtaca tgcgttgcac gtcgtctggg tcggttacgt cggcgtggac
     1981 gtagatgccg cctatcttgg aggccgcctc ctgacctggc tcgtctgcca gatccgcaat
     2041 gcacacg
//
LOCUS       DATMVF010000195         6312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_434369, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000195 DATMVF010000000
VERSION     DATMVF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434369"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VN178_09695"
     CDS             complement(<1..1459)
                     /locus_tag="VN178_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWS81286.1"
                     /translation="MMGEIFALRRLRWKLTLSYTLVAAVTLLALELILAGVVVAFLNS
                     ALLPRLVVQNFRDEVAPRLERPMNQNPPDTEAIRERLVGFADDSGVRGADGPPPPPTD
                     LSNYTADQGALLVVDDRRRLLVSVPEMRGFPEGRHFDAGSISGLEPLVNAAFDGEENS
                     GQLSSNVGGQVVTVTPIEGKKGRVAGVLVGTLRLPNLTAPLLITVGIGAIFLAVPAAF
                     LGALFGFFTAWSLTRRIGRLVRAARSWSRGDFSVTTKDRSRDELGQLSRELNNMAAEL
                     ENLLQARGELATLEARNRFARDLHDSVKQQVFATSLQIAAARALIEKDVGSAESHLSQ
                     ADELVRQAQKELNILIQEMRPAALEGKGLAAALREYVVRWSEGAEIPADFRVQGERRV
                     PLEVEQALFRVAQEALANVARHSGAGHAEVDLIYAADTVTLHVADDGRGFDPSRNPGG
                     GFGLQSMRERLVRLGGHVNVESAPGEGTRVECFCPV"
     gene            complement(1597..4650)
                     /locus_tag="VN178_09700"
     CDS             complement(1597..4650)
                     /locus_tag="VN178_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWS81287.1"
                     /translation="MEELFGVPTQQLLWILLAIFGAAALILGLSALRNRVSFRMAARN
                     LPRRRTQTILVVLGLMLATMLFSASFTTGDTLTNSLRMQSLENLGQVDVQVQADGAGA
                     SGQQEFGDTSSERANYFDAKVAGEVRDRLSDENRVAGVAPAAIETVPVTARGSDLSEP
                     STDVLSLDQEQMEGFDTLTASSGKTLDPSDLGKDEIYLSVDAAEGLDVGNGDVIEATF
                     AQKPGATAAPNAPPAAARGALAVPRPSELTVAGVYESGANPASSTSMVMPLQRLQERV
                     GEEGRINAVLITHRGPAIEGAAGTDATKDALRPLLEEYDLQAEPVKREAIDQADQGGE
                     TFTSIFLLFGQFSVAAGVLLIFLIFVMLAAERKHELGIARAVGMRRGHLMRMFAFEGA
                     LYALLASALGSVAGVGVGWLMVRVIGKAFAGSGFEISFATSPENVLIAFCLGMVLTFA
                     VVLISSWRVSRLNVVKAMRDIPEPDRKGRSAKGVLLALATPLAGAGLLWQGLQAEQMG
                     LYMLGLSLIIVGAALVARVLRVPERISFTVAGMLLLGLWLLPLPFAPSGMTEGIDLFF
                     ISGVMIVLAGVWIIIYNSDLLLGAVVALFGWLRGMPPVLRAAVSYPMQSRFRTGMTLA
                     MFSLVVFTLVTMSFITAAFGSIFEDTDRLSGGFDVRADAGYAAPIPDMNEALNDKKGV
                     NADDFTAVGTLTGLTVDAKQRDTSRKPQSLFLQGVDGGYTRNVGYRFKSTVPEYDTAD
                     EVWNALRTEKDTAVITSDLAPSRSNFVGDEEPQVKLSGFYSEDPTLPDDLYIQVEDPQ
                     TGRTRDLHVIGVLEDTAFFAPGVTASRATVDDLAGSPLPAQSYQFRLAEGVDPVAVAK
                     DLEKAFAQNGLQAVAIEKEIEEGSATQGLFNNLLMGFMGLGLLVGIAALGVIAARSVV
                     ERRQQIGMLRALGFQRGQVRLAFLLESSFVALLGIGLGVALGAALSVGIVDSFAEQVS
                     GIRYTVPWSTLGVIVGLAYFASLLTTFLPASQASRVYPAEALRYE"
     gene            complement(4651..5364)
                     /locus_tag="VN178_09705"
     CDS             complement(4651..5364)
                     /locus_tag="VN178_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS81288.1"
                     /translation="MEETAADFIIEATGVQKVYVAGGLRVRALQGVDLGVRRGEMVAI
                     MGPSGSGKTTLLNVLSGLDDLSDGEVYVDGESIKGMSDRRRTRFRAERMGFIFQSYNL
                     IPVLSAIENIELPLLVAGKKPKKARQWAQEAIEMVGLADQARKRPAEMSGGQQQRITV
                     ARSLVNNPAIVWADEPTGALDSETSKGIMDLLVRMNEQEQQTFVLVTHDAAVAARAHR
                     TIRMRDGRIASDESNGNGA"
     gene            5783..>6312
                     /locus_tag="VN178_09710"
     CDS             5783..>6312
                     /locus_tag="VN178_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HWS81289.1"
                     /translation="MLRTVTATRYVTPLREGGSLPAIVEADDLGTYVLKFRGAGQGRK
                     ALVAELVAGEIARRLDLLVPEVVLAYLAPELGRSEPDPEIQDLLQRSTGLNLALDYLP
                     GSLGFDPLVEPPDSNLASRIVWFDALVTNVDRTPRNTNLLTWHDRLWLIDHGASLYFH
                     HNWPARDGMALEAASRR"
BASE COUNT         1097 a         2114 c         2042 g         1059 t
ORIGIN      
        1 gcaccggaca gaagcattcc acgcgtgtcc cctctccggg cgcgctctcg acgttgacgt
       61 gtccgccgag cctgaccagc cgctcgcgca tgctctgcag accgaagccc ccgcccgggt
      121 tccgcgaggg gtcgaagccg cggccgtcgt cggcgacgtg cagggtgacg gtgtccgcgg
      181 cgtagatgag gtccacctcg gcgtgaccgg ctccgctgtg cctggcgacg ttggccagcg
      241 cctcctgggc gacgcggaag agcgcctgtt ccacctccag gggtacccta cgctcgccct
      301 ggacccggaa gtcggcaggg atctcggccc cttccgacca gcggacaacg tactcgcgga
      361 gcgccgccgc gaggcctttc ccttccaggg ccgcgggacg catctcctgg atcaggatgt
      421 tgagttcttt ctgggcctgg cgtacgagtt cgtccgcctg ggagaggtgt gactcggccg
      481 agcccacgtc cttttcgatc agggcgcggg ccgcggcgat ctgcagcgag gtcgcgaaga
      541 cctgctgttt gacggagtcg tgcaggtcgc gggcgaaacg gttgcgggct tccagcgtcg
      601 caagctctcc ccgcgcctgg agcaggttct ccagctcggc cgccatgttg ttgagctctc
      661 gcgagagctg gccgagctca tccctagagc ggtccttcgt cgtcacggag aagtcccccc
      721 tgctccacga gcgggccgcg cggacgagac gtcctatgcg acgggtcagg ctccaggcgg
      781 taaagaagcc gaagagcgca cccagaaagg cggccggaac cgccaggaag atcgcgccga
      841 tccccaccgt gatgaggagc ggcgccgtga ggttcggcag ccgcagcgtg ccgacgagaa
      901 ccccggcgac ccggcccttc ttgccctcaa tgggcgtgac ggtcacgacc tggcctccga
      961 cgttggaaga gagttggccc gagttctcct caccgtcgaa cgcagcgttc accagcggct
     1021 cgaggccgga gatgctgccc gcatcgaagt gcctgccttc cgggaaacct cgcatctcgg
     1081 ggactgagac cagcaggcgg cgtctgtcgt cgaccacgag gagggcgccc tggtccgccg
     1141 tgtagttgct caggtcagtg gggggtggcg ggggcccgtc ggcgccacgt acaccggagt
     1201 catctgcgaa gcccacgagt ctctctcgta tagcttcggt gtccggcgga ttctggttca
     1261 tgggcctctc cagacgtggc gctacttcgt ccctgaagtt ctgcaccacc aacctcggaa
     1321 gcagggccga gttcagaaag gcgactacca cgccggccag gatcaactcc agcgcgagca
     1381 gcgtcaccgc ggccaccagc gtgtagctga gcgtcagctt ccaccgcaac cgccgcagag
     1441 cgaagatctc acccatcatc gcagatgccc gttctcatga ctcaggccag tttagtaccc
     1501 tgcggcacgc agcgcatcgg cccgcggacg caacgacgct cggtcccggg gacgaaagga
     1561 gggtccgcgc ggaccctccc ttagaatcgg gcctctctac tcgtaacgca acgcttcggc
     1621 aggatagacc ctggaggcct ggctggcagg gaggaaagtg gtgagcagcg aggcgaagta
     1681 ggcgaggccg acgatcacac ccagggtgct ccatgggacc gtgtagcgga taccggagac
     1741 ctgctccgcg aaagagtcga cgatcccgac cgagagcgcc gcgccgaggg ccacgccgag
     1801 tccgatgcct agcagggcca cgaaagacga ctctaaaagg aaggcgagcc gcacctgtcc
     1861 ccgctggaag ccaagggccc ggagcatccc gatctgctgc cttctctcga ccacggagcg
     1921 ggccgcgatc acgccgaggg cagcgatgcc taccaggagg ccaagaccca tgaaacccat
     1981 gagcaggttg ttgaagagtc cctgcgtggc cgaaccttct tcgatctctt tctctatcgc
     2041 gacggcctgg aggccgttct gggcgaaggc cttctccaga tctttcgcga cggccaccgg
     2101 atccacccct tccgccaacc tgaactggta gctctgcgcc ggcaggggag atcccgcaag
     2161 gtcgtcaacg gtcgcccgcg aggccgtcac gccaggcgcg aagaacgccg tgtcctccag
     2221 caccccgata acgtgcagat cccgcgtcct ccccgtctgg ggatcctcga cttgtatgta
     2281 gaggtcgtcg gggagggtcg ggtcttcgct gtagaagccc gagagcttta cctgaggttc
     2341 ctcatcgccg acaaagttgg acctgctcgg cgcgagatcc gacgtgataa ccgccgtgtc
     2401 cttctcggtc ctcagcgcgt tccacacctc gtccgccgtg tcgtactcgg ggacggtgga
     2461 cttgaatcgg tagccgacgt tcctggtgta gcctccgtcc acgccctgca ggaacaggct
     2521 ctgcggcttc cggctcgtgt ccctctgctt cgcgtccacg gtgagcccgg taagggtgcc
     2581 gaccgcggtg aagtcgtcgg cgttcacgcc cttcttgtcg ttcagggcct cgttcatgtc
     2641 cgggatcggg gccgcgtagc cggcgtcggc gcgcacgtcg aagccgccgg agagccggtc
     2701 cgtgtcctcg aagatggaac cgaaggcggc ggttatgaag ctcatggtca ccagcgtaaa
     2761 gacgacgagg gagaacatgg cgagggtcat gccggtacga aatcggctct gcatcgggta
     2821 ggatactgcg gcccttagga cgggcggcat cccgcgcagc cagccgaaaa gggcgacgac
     2881 ggcgccgagg agcaggtctg agttgtagat tatgatccac acgccggcca gcacgatcat
     2941 aacgcccgag ataaagaaga ggtctatgcc ttccgtcatt ccgctgggcg cgaacgggag
     3001 gggcaggagc cagagcccga gcaagagcat ccccgccacc gtgaaggaga tccgctcggg
     3061 cacccgcagc acgcgggcca cgagcgcggc cccgactatg atgagcgaga gccccagcat
     3121 gtacaggccc atctgctccg cctggaggcc ctgccagagg aggcccgccc cggcgagcgg
     3181 ggtcgcgagc gcgaggagca cgcctttggc gctgcggccc ttcctgtcgg gctctgggat
     3241 gtcgcgcatg gccttgacca cgttcaggcg gctcacccgc caggaggaga tcaggaccac
     3301 cgcgaacgtg agcaccatgc cgaggcagaa cgctatgagg acgttctcgg ggctcgtggc
     3361 gaaggatatc tcgaagccgg agcctgcgaa cgctttgccg atcacgcgca ccatcagcca
     3421 ccctaccccg acgccggcga cgctaccgag ggcactcgcg agcaaggcgt agagggcacc
     3481 ttcgaaggcg aacatccgca tcaggtgccc cctgcgcatc cctacggcgc gcgctatccc
     3541 gagctcgtgc ttgcgctccg cggcgagcat gacgaagatc aagaagatca agagcacgcc
     3601 cgccgctacg gagaactgcc cgaagagcag gaagatgctc gtgaacgtct ccccaccctg
     3661 gtcggcctga tctatggcct ccctcttgac gggctcggcc tgaaggtcgt actcctcgag
     3721 tagcggcctg agggcgtctt tggttgcgtc ggtgcccgcg gcgccctcga tggcaggccc
     3781 tctgtgggtg atgaggacgg cgttgatcct gccctcctcc cccacccgct cctgcagcct
     3841 ctgtaacggc atgaccatcg aggtcgaaga ggccggattc gccccactct cgtagacgcc
     3901 agccacggtc agctccgaag gccgcgggac ggccaaagca ccacgggcag cggccggggg
     3961 cgcgttcggg gcggcggtcg cgcctggctt ctgggcgaag gtggcttcga tcacgtcccc
     4021 gttcccgacg tcgagacctt ctgcagcgtc aacgctcaag tagatctcgt ctttaccaag
     4081 gtcggaggga tccagcgtct tgcccgagga ggcagttagc gtatcgaagc cctccatctg
     4141 ctcctggtcc aggctgagca cgtccgtgct cggttcgctg aggtcgctgc ccctggccgt
     4201 tacagggacg gtctcgatgg cagcgggagc cacgcccgcg acgcggttct cgtcggagag
     4261 tcggtcgcgc acctcgcccg ccaccttcgc atcgaagtag ttggcccgtt ctgaggatgt
     4321 gtccccgaac tcctgctggc ccgaggcgcc cgcgccgtcg gcctgcacct gaacgtcgac
     4381 ctggcccagg ttctccaggg actgcatgcg gagggagttg gtcagggtat ctcccgtggt
     4441 gaaagaggcg gaaaagagca tggtagcgag catcaggccg agcacgacga ggatggtctg
     4501 ggtgcggcgg cgcggcaggt tacgggccgc catcctgaag gagacgcggt tacgaagggc
     4561 ggagagaccg aggatcaggg ccgctgcgcc gaagatggcg agaagaatcc acagtagttg
     4621 ctgcgtcgga acgccgaaga gctcttccac ttacgctcca ttcccgttcg actcgtcgct
     4681 cgcgatgcgg ccgtcgcgca tgcggatcgt acggtgcgcc ctggccgcga ccgcggcgtc
     4741 gtgggtgacc aggacgaagg tctgctgctc ctgctcgttc atcctgacca ggagatccat
     4801 gatccccttc gaggtctccg agtccagggc gccggtcggt tcgtcggccc agactatggc
     4861 cgggttgttc acgagcgagc gggcgacggt tatccgctgc tgctggccac cgctcatctc
     4921 ggccggacgt ttcctggcct gatccgcgag ccccaccatc tcgatggctt cctgcgccca
     4981 ctggcgggcc ttcttcggct tcttgccggc gaccagcagg ggaagctcga tgttctcgat
     5041 cgcggagagg acggggatca ggttgtagga ctggaagatg aagcccatcc tctcggccct
     5101 gaagcgcgtc cgtctcctgt cggacatgcc ctttatggac tcgccgtcga cgtagacctc
     5161 gccgtcggag aggtcgtcga ggccggagag aacgttcaac agcgtggtct tccccgaccc
     5221 ggaaggcccc atgatggcga ccatctcccc gcgcctgacg ccgaggtcaa caccctggag
     5281 cgcccgcacc cgcagtccgc cggcgacgta aaccttctgg acgcctgtgg cctcgataat
     5341 gaaatctgcc gcggtttctt ccacgaaacc tccccgctcc gtcgaaactg actctcgcca
     5401 cccgagcatg actccctcct ccgtcgccta cactctacgc agtaaccata ggcgggttgc
     5461 tccggactca catcggacct cggatcgaag ttgactaggt ctacggacgg aacgcctcca
     5521 cgccaagacc atcccggccg cccctcccct gacgcccagg aaggaatacc gtttcgggat
     5581 ggaaagcagc cagaacgaat aaagggtcgc cggggaggat ggggagaacg gcgacccttt
     5641 gactgattgt accggcacag gcgacgattc cctaccccga aggtagcgct acggcccgcg
     5701 gcgtctgtcg agcgtgggtg cgagagtcgg tagaatagtt ggcgtcggtt ctcccgctaa
     5761 cacctacgcc gaggtattca gcttgctcag aacggtcaca gcgacacggt acgtaacgcc
     5821 cttgcgcgag gggggttcgc tgcccgcgat cgtcgaggcg gacgatctcg ggacctacgt
     5881 cctcaagttc cgtggtgctg gacagggccg caaagcgctt gtggctgagc tcgtggcggg
     5941 tgagatcgcc cgccgcctcg acctcctcgt gccagaggtc gtgctcgcct acctcgcccc
     6001 ggaattgggc cgctctgaac ccgaccccga aatacaggat cttctacaaa ggagcacggg
     6061 cctgaacctt gccctggact acctgccggg ctctctcggc ttcgacccgc tcgtagagcc
     6121 gccggattcc aacctcgcct cgcgcatcgt ctggttcgac gccctggtga ccaacgtcga
     6181 ccgcaccccg cgcaacacga acctcctgac ctggcacgac cgcctctggc tcatagacca
     6241 cggtgcttcg ctgtacttcc accacaactg gccagcgaga gatgggatgg ccctggaggc
     6301 cgcgagccgg cg
//
LOCUS       DATMVF010000196        11064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_248834, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000196 DATMVF010000000
VERSION     DATMVF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248834"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VN178_09715"
     CDS             <1..423
                     /locus_tag="VN178_09715"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HWS81290.1"
                     /translation="PVPGWEHTFQEDGGVVRAVTFSGGEIRPREFQQFLVQAQAPDKP
                     GKYPWKAFQTYEDGSVVEWVGASESEEPASVVEVVSAGSEQSQSSPKPSEASASQKEA
                     SSDAAFLAETGGTNPAVYAGLGFVGLVVSALLARRLLG"
     gene            427..1863
                     /locus_tag="VN178_09720"
     CDS             427..1863
                     /locus_tag="VN178_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HWS81291.1"
                     /translation="MPKREAIRRGVLLAGLACAWLLLACAPALAHASLVEASPPQGSE
                     VSNPPDRVELRFSEPVDAEFDPVMVRGADGTRVDTRDAHVDPDDARVVLAYLERVPEG
                     SYTVKWRVTSIDGHVVEGRYAFAVAATAKNWPTKDAGEKDTSAEDAGAQSGHHGGHHG
                     GGAERVAAPHQREPISESAAGRATSSGAASIEHGLALAASAFLAGLAPFVALVWLPAS
                     RQTGAAGHGALRPFGVLAWALLCVLAVAGVGELSAYAVRASGEPFSTGLFAQTLFGSR
                     VGEVWLIRLALALLTAAAITAAAGSGRTWEWGVATATGGLLLMTLTGLSHAAATGRIL
                     PLVADWTHAVAAAVWMGGLLGFVVALFFGQLRSLPPDRQAKLRERSVRRFSTVATIAV
                     AVLAATGLYAALLHVPSPQALVDTPYGRALLVKLGLLALVLAIGTCNLLLRGRGPFGR
                     LMTVELLLALGLFVATGFLTSLPPASGT"
     gene            2065..2658
                     /locus_tag="VN178_09725"
     CDS             2065..2658
                     /locus_tag="VN178_09725"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWS81292.1"
                     /translation="MEHQERTDKFTEEVAHRFAGYTVCDQDYEKIGNVDDLFLDQSDH
                     PEYIGVRMGFLGTRTTLIPVQMARVNEARQLIEVAADKQTLKNGPTFDDDRQITPQFE
                     NQLYSYYGLKRPSTIEHSGTYAAHSAEQSTQTTGRIKEAGGTKDNVTDELDEAAGRGR
                     RRRVVRKEAAPEEVGQPGSGGVKANVRDEWAESQERR"
     gene            complement(3356..4042)
                     /locus_tag="VN178_09730"
     CDS             complement(3356..4042)
                     /locus_tag="VN178_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81293.1"
                     /translation="MESANPDLRHPTAKFAEPLPYGYIYAGMTVDPPGRAPFVRGSAR
                     RDEALEKLKIVAGQLEALDEVVRATVYRAVLIPPIEDAPRFDVTVLLKTTSPEEISGV
                     QTSEPYRQLDADFVMGARNTRRIGDTEENLSGTFLFNHFTAEDPERALEAWEGLTGWY
                     TTKTGVDNSTLLQPIGEAPYAFVNYVRLPRGPVRFMLDQLIRPSFHTFVRTKLRANGM
                     VALPLLCEVV"
     gene            complement(4242..4469)
                     /locus_tag="VN178_09735"
     CDS             complement(4242..4469)
                     /locus_tag="VN178_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81294.1"
                     /translation="MAIEMTGGKIVNERGTVVTFRQRCEACGYVFDWNKTTIVPAYGS
                     RKVRPFTCPECGNYQEVEVRYLHKGPHQENT"
     gene            complement(4577..4840)
                     /locus_tag="VN178_09740"
     CDS             complement(4577..4840)
                     /locus_tag="VN178_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81295.1"
                     /translation="MESKNSLLLGIKYEAARIGEVGEAALAGIEEQIRREVGDGWESD
                     DHVIRGRNAVRREYGLPGLGEPGDRPSPVTDPIRPLWLRLFGK"
     gene            complement(4882..6081)
                     /locus_tag="VN178_09745"
     CDS             complement(4882..6081)
                     /locus_tag="VN178_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWS81296.1"
                     /translation="MIFELSKKARGYRERVEAFMDELIYPNETKYHEQLGASGSRWSV
                     PRVMEELKEEAKARGLWNLFLPDSDLGAGLTNLEYAPLCEVMGRSPIAPEVFNCSAPD
                     TGNMEVLVRYGTEEQKDRWLRPLLDGEIRSCFAMTEPDVASSDATNIRARIERDGDEY
                     VVNGRKWWISGAGDPRCRISIFMGKTDPDAARHEQQSMILVPLDTPGVEIERTLPVFG
                     YDDAPHGHAEISFEDVRVPRENIIWEEGKGFAIAQGRLGPGRIHHCMRLIGVAERALE
                     MMCERVKGRETFGKALAEQGVVQAWISDSRMEIDQARLLTLKAAHMMDTVGNKEARAE
                     IAMIKVVAPNVALNVLDRAIQAHGGAGVSDDFPLAYLWASSRTLRLADGPDEVHRAAV
                     ARLELKK"
     gene            complement(6332..7102)
                     /locus_tag="VN178_09750"
     CDS             complement(6332..7102)
                     /locus_tag="VN178_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS81297.1"
                     /translation="MELGLENKAFVVGGASSGLGRAVAEQLVAEGARVLLVARGEDAI
                     EEVARDLGERAEQCVADVSHASGVDKVATVAIDRFGTLDGVLVNAGGPPFGPALELTD
                     EQWLDAFRLLLGGPVRLLRTLVPRMNDGASVLFITSSSVREPIKGLDASNVLRPGVAA
                     LVKCLAQEVGPRVRVNSMAPGRIDTDRSRSLDEARAQDLGISTEEQRRNFSEGIPLGR
                     YGEPAELGRAAAFLLSPAASYITGVSLQVDGGLVSAVP"
     gene            complement(7176..8159)
                     /locus_tag="VN178_09755"
     CDS             complement(7176..8159)
                     /locus_tag="VN178_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWS81298.1"
                     /translation="MTSRPTGGLLLLLLLAVALTILGCGAGSADDGVKIAGDQFRLSG
                     TQRDAQFAVGSESFTEQEVLGNIAIEVLEAADATVIDRTGLGGNEEVRQALESGEIDL
                     YWEYTATGWLVHLSETDSISDPREQYEAVARQDLEENGIKWLDPAPGNDTYAIAASEK
                     TGRDLGVETISDLKRLVEDSPEEATLCLNNDDDFLTRFDGLPGLERAYGFEFPEENLI
                     ETSVDAVYDATDEAEICNFGVVFTTSGFIWELDLRLLEDDKEFFAVYNPALTVRQNVY
                     KQYPQLRKIFAPISEKLDTETLRKLNYAVDVQGKSPESVARMWLRENGFIE"
     gene            complement(8156..9751)
                     /locus_tag="VN178_09760"
     CDS             complement(8156..9751)
                     /locus_tag="VN178_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HWS81299.1"
                     /translation="MQDTQGDRHEVGVVFWVSAAIALAFILWGVIAPTNFGTVTQAVF
                     DWVVSNLGWFYLLAGNFFLVFVVILALSRYGRFKLGKEGDRPEFRTFAWFAMLFQAGM
                     GPALIFWGVAEPLSHYSAPPFGMAQPATTEAAQVGIQYSYFHWALHPWAMYAVVGLVV
                     GYFSFRRDEPGLISPVFRPLLGDRVDGPWGKTIDVMSVLAVLFGVAVALGQAGLQLTA
                     GLGETFGTPTGIAVQLAVIVVTTVAFMISASTAIEKGINYLSQISMYVAAVLLVFFLV
                     VGPTATQLGALTQGIGDYLGGIVPMSMRLESFDPENTWLGSWTVFYWSWWIAWCPYVG
                     LFIARISRGRTVRQFVLGTVIGPSVVTFVWIAVFGGSALHLAQTKGEGIAETVAADPA
                     SGMFVFLNQFPLALPMSILTLLVLWIFFVAGADAGTVVLGSLSTGGPQEPKRWIKLAW
                     GAAMAAIACILLVAGGLGALQSASVLFGAPFAVIMVLMCIVFYRHLRSEAREREDAPP
                     AARRPAAGDPTSGQAFASGEEGV"
     gene            10137..>11064
                     /locus_tag="VN178_09765"
     CDS             10137..>11064
                     /locus_tag="VN178_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWS81300.1"
                     /translation="MQMIKNSVGIWAFGPAVTRFVPPGYHNEVAEEPMVEKTERVAGG
                     LHDLLEGLEYHYPGEIHEDNVEDVLRVLAAHGMDLPVIAAGLHPDPTYALGAFINPDE
                     RLRRQGIDTLKRGIDLCAEIRANFIIWPGAEGYNYTFQRPYAEIWRRFVEGVAELTDH
                     ANEKDVKVFLEHKNSEPAMKILMQNLGMTLFTIQKVNALGVDTTNLQVNMDWQHLIMN
                     GENLAEYADLLASEHKLGHQHGNDGWGTFDDDNVVGTNFFMQTLELAQTLQDTGYGEE
                     GEIIGFDLYPYTEDQVAAVRRAIVQWEFIWDLA"
BASE COUNT         2067 a         3554 c         3405 g         2038 t
ORIGIN      
        1 cccgtgcccg ggtgggagca caccttccag gaggacggcg gtgttgtgag ggccgtgacc
       61 ttctccggcg gagagatacg cccgcgggag ttccagcagt tcctcgtgca ggctcaagcc
      121 cccgacaaac caggcaaata cccatggaag gcgttccaga cctacgagga tggcagcgtc
      181 gtagagtggg taggcgcctc cgaatcagag gagccagcct ctgtggtcga ggtggtctcc
      241 gccggatctg agcaatccca gtccagcccg aagccctcgg aggcgagcgc ctcccagaag
      301 gaagcgagta gtgatgcggc gttcctagca gagacgggcg ggacgaaccc ggcagtttat
      361 gcagggcttg gcttcgtcgg actggtagtt tcggctttgc tagcgagacg cctcctgggg
      421 tagaggatgc cgaaaaggga ggcgataagg cgcggcgtgc tccttgcggg tctggcctgc
      481 gcctggctcc tgctggcttg tgcaccggca ctcgcccacg cgagcctcgt ggaggcctct
      541 cccccgcagg gaagcgaagt gtccaacccc cccgatcgag tagaactacg cttcagcgag
      601 cccgtggacg cggagttcga ccctgtgatg gtgcgtggtg ctgacggtac ccgagtcgac
      661 actcgcgatg cccacgtgga tccggacgac gcgagggtcg tgctggcgta cctcgagagg
      721 gtgcctgagg gatcttacac cgtcaagtgg cgggtcacct ccatagacgg gcacgtcgtg
      781 gagggcagat acgccttcgc cgtggcagcc acggcaaaga actggccgac taaagatgct
      841 ggggagaagg atacctccgc cgaagacgca ggggcgcaga gcggacacca tggtgggcac
      901 cacggaggag gtgcagagag agtagcggct ccccaccagc gagagcccat ctctgagtca
      961 gccgcgggac gcgccacaag tagtggggct gcaagtatcg aacacgggct cgccctggct
     1021 gcgagcgcgt tcctcgccgg cctggcgcca ttcgttgcgc tcgtgtggct cccggccagt
     1081 cggcagaccg gcgccgccgg ccacggcgcg ctccgcccgt tcggggtgct ggcgtgggcg
     1141 ttgttgtgcg tgctcgccgt cgctggcgta ggcgagttgt ccgcctacgc ggtccgcgcc
     1201 tctggcgagc ccttcagcac ggggctattc gcacaaacgc tcttcggcag cagggtaggc
     1261 gaggtctggc tcatccggct cgccttggcg ctgctgacgg ccgcagcgat caccgcggcg
     1321 gcgggttcgg ggcggacttg ggagtggggg gtggcgaccg cgaccggggg cttgctgctg
     1381 atgaccctga cggggctcag ccatgcagcc gccacgggcc gcatcttgcc cctcgtggcg
     1441 gactggaccc acgccgttgc ggcggcggtg tggatgggtg gcttgctggg cttcgtcgta
     1501 gctctgttct tcgggcagct tcggagcctg ccaccagacc ggcaagcgaa gctgcgcgag
     1561 cgctcggtac gccgcttctc gaccgtggcc accatcgccg tcgcggtcct tgccgccact
     1621 ggcctgtacg cagccctcct ccacgtccct agtccgcaag cgttggtcga tacgccctac
     1681 ggccgcgccc tgctcgtgaa actcggactg ctagccctcg tgctcgccat aggtacatgc
     1741 aacttgctgc tgcgcggtcg tggaccgttc ggtcgcctga tgaccgtcga gttgctcctg
     1801 gcgctcgggt tgttcgtggc cacgggcttc ctgaccagcc tgccccccgc aagcggcact
     1861 tagcaggcca gggctccctc cggagcgcta ttcaccggag tacgtggaat atagattctg
     1921 cgaacttcgc ctttacgcag ttctaagaag ttcgcatagc cccattccac tactactcag
     1981 cagccgcgtt tagctttgcc aggagaacgg gtaggcctgt tagtagtatg ttggcacgga
     2041 gcatcttatg tggaggtatt taccttggag caccaagagc gcactgacaa gtttacggag
     2101 gaagtagctc atcgcttcgc aggctacacg gtctgcgacc aagactacga gaagatcggc
     2161 aacgtagacg acctgttcct cgaccagagc gaccatcccg agtacatagg cgtgaggatg
     2221 ggcttcctgg gaacgcgtac caccttgatc ccggtccaga tggcaagggt caacgaggcg
     2281 cggcagctca tcgaggtcgc agcagacaag cagacgctca agaacggccc caccttcgat
     2341 gacgaccggc agatcacccc tcagttcgag aaccagctct actcctacta cggccttaag
     2401 cgtccttcta ccatcgagca cagcggcacc tacgcagccc actctgccga acagagcacc
     2461 cagacaacgg gccgcattaa ggaagccggt gggacgaagg acaatgtcac cgacgagctg
     2521 gacgaagccg ccgggcgagg caggcgcagg agagtagtcc gcaaggaggc cgcccccgag
     2581 gaagtggggc agcctggctc cgggggagtc aaggcgaatg tccgggacga gtgggccgag
     2641 tcccaagagc gtcgctagcc ggaaaagagc caatccgcga aagctgggcc ggagtcctaa
     2701 ggggctccgg ccctttctct tgcccttatt cacctgagtg cgtggaatag attttctcgg
     2761 aagctcatat tcaagatcgt gtatagcctc accccggtag ccctgagaac acccgtccaa
     2821 gcaccctgca cagccccgcc tcgataccgc ttgtgacacc gatggataga tatgcgcgat
     2881 catggatgtt ctttctacag acatcagtct gtacttcgga gagaacaccc ccacgccctt
     2941 ccctaatgct ccaggtagcg ggacgggact atgctgaact tctgtgagaa cccgaaacag
     3001 gcgaagttcg cagaattccc cagcccgttg aaaaactcct caatgccacc ttggagcccg
     3061 gttcaaggac tcaaagaccc tctttccgca gtgttttggt cttgatttgg cggtgttgaa
     3121 ttcgtttctg atctgccaag tggccagata gtgacttttc caacaggccg tcatattcca
     3181 cgcactccgg tgaataagat caagagataa gccgaggttg ctacgctccg taacctcccg
     3241 tagtgtcttg gtcgtaaaga gggacataca gcctggccgc ggtaaagcct cgctgtcggc
     3301 tgccgcaggg tcaaatgctc ggggcgctgc atcttccggc cgatcaccga accgctcaga
     3361 cgacctcgca cagcaggggc aaggctacca tgccgttcgc gcgcagcttt gtccgcacaa
     3421 acgtgtggaa gctcggcctg atcagctggt cgagcatgaa ccggaccggc ccgcggggca
     3481 ggcgcacgta gttgacgaag gcgtagggcg cctcgccgat gggctgcaga agggtggagt
     3541 tgtccacgcc ggtcttggtt gtgtaccatc cggtgaggcc ctcccatgcc tccagcgcac
     3601 gctcggggtc ttcggccgtg aagtggttga acaagaaggt ccccgagagg ttctcctcgg
     3661 tgtcgccgat ccgcctggtg ttgcgcgcac ccataacgaa gtcggcatcc agttgcctgt
     3721 acggttccga ggtctggacc ccggagatct cctcaggcga ggtggtcttt aggaggaccg
     3781 tcacgtcgaa gcgcggcgcg tcctcgatcg gcgggatcag gacggcccgg tagacggtgg
     3841 ctcggaccac ctcgtcgagg gcctcgagtt ggccggcgac gatcttgagc ttctcgagcg
     3901 cctcatcgcg cctggcactc ccgcgaacga acggagcgcg gcccggcggg tcgaccgtca
     3961 tgccggcgta gatgtagccg tagggcagag gctccgcgaa cttggccgtc ggatgtctta
     4021 gatccgggtt ggcggactcc atggaggctc ctctaaaagt gagagcgcac gcttaccaga
     4081 gcatctgtag tataaacgag cgctcactta ccatgataag catctgaaac cacccgacat
     4141 taccgtccca ccgtcattcc cacgaaagcg ggaatccacc gcagcggacg ctccgcgtct
     4201 ccaaccacaa ccgatctacc ggatcgagtg tgatgtgggg atcaggtgtt ctcctgatgc
     4261 ggacctttgt ggagatatcg gacctcaact tcttgataat tcccgcactc ggggcaggtg
     4321 aaaggtcgta cttttcggga gccgtatgca ggcacgatgg tagtcttgtt ccagtcgaag
     4381 acgtagccgc acgcctcgca ccgctgcctg aaggtaacga ccgtgccgcg ctcgttgacg
     4441 atctttccac ctgtcatctc tatcgccatc cagcctccgc cagggaaacg cgcctacgtc
     4501 cctattgtat gcccacagca tccttcagat cgagaaccat agagccggta tacaccaccc
     4561 actgacaccc gaaaacctac ttcccgaaca gcctcagcca gagcgggcgt atcgggtctg
     4621 tgactggaga gggcctgtcg cccggctcgc cgagtcctgg tagtccgtac tcccgcctta
     4681 cggcgttgcg gccccttatc acatggtcgt ccgactccca gccgtcgccg acctctcttc
     4741 ttatctgctc ctcgataccg gcgagtgcgg cttcaccgac ctcaccgatt ctcgcggctt
     4801 cgtacttgat gccgaggagc aaggagttct tgctctccat ttctcctcct ctgttggtta
     4861 tgtcactgtc gtgggccgac gctatttctt gagttcgagc ctggccacag cggcgcggtg
     4921 aacctcatcg gggccgtcgg cgaggcgcag ggtgcgggag ctggcccaga ggtaggcgag
     4981 cgggaagtca tcagagaccc ctgcgccacc gtgggcttgt atggcccggt cgaggacgtt
     5041 caatgccacg ttgggtgcga cgactttgat catggcgatc tccgccctgg cctctttgtt
     5101 gcctaccgta tccatcatgt gggccgcttt caaggtcaaa aggcgcgcct gatctatctc
     5161 catcctggaa tcggagatcc aggcctgcac gacaccttgc tccgcaagcg ccttcccgaa
     5221 cgtctcccgc cctttgaccc gctcgcacat catctccagc gctctctcgg ccacgcctat
     5281 gagcctcatg caatggtgga tgcgccctgg tcccaggcgt ccctgggcga tggcgaagcc
     5341 tttgccctct tcccagatga tgttctcccg tggcacgcgt acgtcctcga aagagatctc
     5401 cgcgtgcccg tggggggcgt cgtcgtagcc gaagacgggg agagtccgct ctatctctac
     5461 accgggcgta tcgagcggga ccaggatcat ggactgctgc tcgtggcgcg ccgcgtcggg
     5521 gtcggtcttg cccataaaga tggagatcct gcaccgcgga tccccggccc cagagatcca
     5581 ccacttcctg ccgttgacga cgtactcgtc cccgtccctc tctatgcgcg ccctgatatt
     5641 cgtcgcatca gaagaggcca catccggctc ggtcatcgcg aaacaggacc ttatctcacc
     5701 atcgagcaac ggcctcagcc agcggtcttt ctgctcctct gttccgtagc gtacgagcac
     5761 ctccatgttg cccgtgtccg gcgcgctgca gttgaagacc tcaggagcta tcgggctgcg
     5821 gcccatgact tcgcacaagg gtgcgtactc caggttggtg aggccggcgc cgaggtccga
     5881 gtcgggcaga aagaggttcc acaaccctct cgccttcgcc tcctctttga gctcctccat
     5941 gacccggggt acggaccacc ggctccccga cgcccctagt tgctcgtggt acttcgtctc
     6001 gttcgggtag atgagttcgt ccatgaacgc ctcgacccgc tcccgatacc cccttgcttt
     6061 cttggagagc tcgaagatca caaccactcc tctcatagcg ggttgcagaa ggattgacct
     6121 tctgcaaccc gcgctgtcag ctcttggctt tcagctgtca gctctttgtt cttgctgatg
     6181 gctcgtcgtg cataaggagg cagccctcat gcacgacgct atcacaacgg ccgaagtgcc
     6241 gattctggca cagcatcatg ggcgtcgcaa tccgggcgat ggccttgggg acgtgaatct
     6301 ggtcatcgta atggggcgag aggcttctgc ttcaaggcac ggcggagacg agcccaccgt
     6361 cgacttgcaa agagacgccg gtaatgtacg aggcggctgg ggagagcaag aaggccgccg
     6421 ccctccccag ctccgcaggt tcaccgtagc ggcccagagg aattccctcg ctaaagttgc
     6481 ggcgctgctc ctctgtggag atgcctaagt cctgggcccg cgcctcgtcg agagagcggg
     6541 agcggtcggt gtctatccgt cctggggcca tgctgttgac cctgaccctg ggcccgacct
     6601 cttgcgccag gcacttgacc agggccgcca ccccggggcg cagcacgttc gaggcgtcga
     6661 ggccttttat cggctcgcgc accgatgatg atgtgatgaa gagcacggag gcgccatcgt
     6721 tcatccgcgg gacgagcgtg cgcagcagtc gcactggtcc gcccaacagc agacggaagg
     6781 cgtcgagcca ttgctcgtcg gtcagctcga gcgctggccc gaagggcgga ccgccggcgt
     6841 tgacgagcac gccgtcgagc gtaccgaagc ggtcgatagc aaccgtggcg accttgtcta
     6901 cgcccgaggc gtgggagacg tcggccacac actgctcggc ccgctcgccg agatctcgtg
     6961 ccacctcttc tatagcgtcc tcaccgcgtg cgaccaaaag aaccctggca ccctccgcga
     7021 ccagttgctc cgccaccgcc ctgccgagcc cgctgctcgc gccgcccacc acaaatgcct
     7081 tgttctccag gcctagctcc acggtcgttc gcctcctcgc ctcgaaaacg cctaccggga
     7141 caatcctaca ccctctcgcg gcgccagaag gggcgctact cgataaagcc gttctccctg
     7201 agccacatcc tggctacgct ttcgggcgac ttgccctgca cgtccaccgc gtagttgagc
     7261 ttccgcagcg tctcggtatc gagcttctcg gagatggggg cgaaaatctt gcgtagctgc
     7321 gggtactgct tgtatacgtt ctgccttacg gtgagcgccg ggttgtagac ggcgaagaat
     7381 tctttgtcgt cttccaggag acgcaggtcg agttcccaga taaagccgct cgtcgtgaaa
     7441 acaacgccga agttgcagat ctctgcttcg tcggtggcgt cgtagaccgc atcgacggaa
     7501 gtctcgatca ggttctcctc ggggaactcg aacccgtagg cccgctccaa gccgggtagg
     7561 ccgtcgaacc gggtcaggaa atcgtcgtcg ttgttgaggc agagcgtcgc ctcctcggga
     7621 ctgtcctcga ccaggcgctt caggtccgag atggtctcga ccccgaggtc cctgcccgtc
     7681 ttctcgctcg cggcgatggc gtacgtgtcg ttgccgggcg cgggatccag ccactttatg
     7741 ccattctctt cgaggtcttg cctggcaacg gcctcgtatt gctctcgggg gtcggatatg
     7801 gaatccgtct cggagaggtg gaccagccaa cccgtggccg tgtattccca gtagaggtct
     7861 atctcgccgg actcgagcgc ctgacgcacc tcctcgttgc cgccgaggcc cgtccggtcg
     7921 atgaccgtgg cgtcggccgc ctcgaggacc tctatcgcga tgttgccgag cacctcctgc
     7981 tcggtgaagc tctccgagcc cactgcgaac tgtgcgtccc tctgcgtgcc gctcagcctg
     8041 aattgatccc cggctatctt caccccatcg tctgcgctac cggcgccgca ccccaggatg
     8101 gtcagggcga ccgcgaggag aagtagcaga aggagtcctc ccgtcggtcg ggacgtcaca
     8161 ccccctcctc tccggatgcg aacgcctggc ccgacgtagg gtctcccgcc gccggtcgtc
     8221 ttgccgcggg tggggcgtcc tctctttcgc gggcctcgct gcgcaggtgc ctgtagaaga
     8281 cgatgcacat aaggaccatg atcacagcga aaggcgctcc gaaaaggacg gaggccgact
     8341 gcagcgcgcc gagacccccg gccacgagca agatacaggc tatggccgcc atcgcagccc
     8401 cccaggccag cttgatccac cgttttggtt cctgcggacc gccggtggag aggctgccga
     8461 gcacgacggt cccggcgtcc gcgccggcca caaagaagat ccagagcacg aggagcgtca
     8521 ggatcgacat gggcagggcc agcgggaact gattcagaaa gacgaacatc cccgaggcgg
     8581 ggtcggcggc gaccgtctcg gctatcccct cgcccttcgt ctgggcgagg tggagggcag
     8641 atccgccgaa gacggctatc cagacgaagg tgaccacgct gggcccgatc accgtcccca
     8701 aaacgaactg cctgacggtg cgcccgcgcg agatgcgcgc gataaacagc ccgacgtacg
     8761 gacaccaggc gatccaccag gaccagtaga agacggtcca gctcccgagc caggtgttct
     8821 ccgggtcgaa ggattctagc ctcatgctca tgggaacgat accgcccagg tagtcgccga
     8881 tcccttgcgt cagggcgccg agttgggtgg ccgtggggcc aacgacgagg aagaagacta
     8941 gcagcaccgc ggccacgtac atgctgatct ggctgaggta attgatcccc ttctcgatcg
     9001 ccgtggaggc ggagatcata aacgccaccg tggtcacgac gatgacggcg agctgcacgg
     9061 ctattcccgt cggggtgccg aaggtctcgc cgagtcccgc ggtgagctgg agacccgcct
     9121 gtccgagtgc cacggcgacg ccgaacagga cggcgaggac cgacattacg tcgatggtct
     9181 tcccccaggg accgtcaacc cggtcgccca gcaggggcct gaagacgggg cttattagtc
     9241 ctggctcgtc ccgccgaaag ctgaagtatc ccaccacgag ccccaccacg gcatacatag
     9301 cccaggggtg caacgcccag tggaaatacg agtactgtat ccccacctgg gcggcctccg
     9361 tcgtggctgg ctgcgccatg ccgaagggcg gtgcgctgta gtgagagagg ggctctgcaa
     9421 cgccccagaa gatcagagct ggccccatcc ccgcttgaaa gagcatcgcg aaccacgcga
     9481 acgtgcggaa ctcgggcctg tcaccctctt tgccgagctt gaacctcccg taacggctga
     9541 gggcgaggat gaccacgaag acgaggaaga agttccctgc cagaaggtag aaccagccca
     9601 ggttggagac gacccagtcg aagaccgcct gggtcacggt gccgaagttc gtcggcgcga
     9661 tcacacccca caggatgaac gccagggcta tcgcggccga cacccagaat acgacgccaa
     9721 cctcgtgcct gtctccctga gtgtcctgca ccgcagacct ccttttccta gattctgccg
     9781 gcgttcgagt cgccggtagc ctcatcgtca cctctgggcg atgcccggta gatgcgacca
     9841 tgcctgatag gggaaccgtg accgcccagc cttcattcga ttggggtgcg acagagcgtc
     9901 gcggcagaaa cgctgcgagc cacgcccgga ccgaaggtcc cgcacttgag cgaagcggct
     9961 caggaacgct gggccgtatg ctacaccagc ttgccaccgg ttactacaag atatgccggg
    10021 caagccttag acccgcgagt agtgggtggc gttcggtaca cagcgaaatc tggttcaggc
    10081 tatgataggc ggcgggagga ttatctctcg ttcgttctga aaccagaggg aagggtatgc
    10141 agatgatcaa gaactccgtt gggatatggg ctttcggtcc tgccgtgacc cgctttgtgc
    10201 cgccgggcta ccacaacgag gtcgcagaag agcctatggt ggagaagacg gagcgcgtcg
    10261 ccggtgggct ccacgaccta ctggaagggc tcgagtacca ctatcctggt gagatccacg
    10321 aagacaacgt cgaggatgtg ctgcgggtcc tcgccgccca cgggatggac ctgcccgtga
    10381 tcgccgccgg cctccacccc gacccgacct acgcgcttgg cgcgttcata aaccccgacg
    10441 agagactccg ccgtcaaggc atagacaccc tcaagcgcgg catagacctc tgcgccgaga
    10501 taagggcgaa cttcatcatc tggcccggcg cggagggcta caactacacc ttccagcgcc
    10561 cttacgcgga gatctggcgt cgctttgtgg agggcgtcgc cgaactgacc gaccacgcca
    10621 acgagaagga cgtgaaagtc tttctcgagc acaaaaactc agaaccggcg atgaagatac
    10681 tcatgcagaa cttaggcatg acgctattta cgatccagaa ggtcaacgcc ctgggggtgg
    10741 atacgaccaa tttgcaggtc aacatggact ggcagcacct cataatgaac ggcgagaacc
    10801 tggccgagta cgccgacctc ctggcctcgg agcacaagct cggccaccag cacggaaacg
    10861 acggctgggg caccttcgac gacgacaacg tggtcggcac caacttcttc atgcagaccc
    10921 tggagctggc ccagaccctc caggacacgg gctacgggga agaaggcgag atcatcggct
    10981 tcgacctcta tccctacacg gaagatcagg tggccgccgt cagacgcgcc atcgtgcagt
    11041 gggagttcat ctgggacctg gcca
//
LOCUS       DATMVF010000197         7736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_20673, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000197 DATMVF010000000
VERSION     DATMVF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20673"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..223)
                     /locus_tag="VN178_09770"
     tRNA            complement(150..223)
                     /locus_tag="VN178_09770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(187..189),aa:Met,seq:cat)
     gene            complement(262..334)
                     /locus_tag="VN178_09775"
     tRNA            complement(262..334)
                     /locus_tag="VN178_09775"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Thr,seq:ggt)
     gene            complement(349..430)
                     /locus_tag="VN178_09780"
     tRNA            complement(349..430)
                     /locus_tag="VN178_09780"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(394..396),aa:Tyr,seq:gta)
     gene            complement(534..605)
                     /locus_tag="VN178_09785"
     tRNA            complement(534..605)
                     /locus_tag="VN178_09785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(571..573),aa:Thr,seq:tgt)
     gene            complement(784..1272)
                     /locus_tag="VN178_09790"
     CDS             complement(784..1272)
                     /locus_tag="VN178_09790"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWS81301.1"
                     /translation="MALILDGYNLIGALDRYGTAGTLDAARDLLINDALKAAGWTGNP
                     LIVVFDAHRSPEPERVETRAGGAVRVIYSAAGESADDVIERLLSRLDGSATIYTADFA
                     LQRTALARGAARATPREFANLLDELPAISRSPEVRRKTRIADRLSLETLRKLEEIRRR
                     GK"
     gene            complement(1263..1973)
                     /gene="rlmB"
                     /locus_tag="VN178_09795"
     CDS             complement(1263..1973)
                     /gene="rlmB"
                     /locus_tag="VN178_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWS81302.1"
                     /translation="MKRETIYGIRPVVEALGSGRRKVFQVIDAVGNEEVAEAASARGV
                     EVKRASRQQVEELARGGVHQGVVARVEPYPYSGLAEILSSPEPLVLVLDGLTDPRNLG
                     AVLRAADGAGACGVVIPKDRAVGVTASAVKSSAGASEHVRVARVTNLRRAIDTMKGAG
                     LWVYAAEAGGAPYTGRDLAGAVAFVLGSEGRGVRRLVREGCDGTVSIPMLGAVDSLNV
                     SVASAVLLYEARRRRGWP"
     gene            complement(1974..3362)
                     /gene="cysS"
                     /locus_tag="VN178_09800"
     CDS             complement(1974..3362)
                     /gene="cysS"
                     /locus_tag="VN178_09800"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWS81303.1"
                     /translation="MSGRTVEVGESGGLGLYICGPTVYDHIHVGNARAPLFWDVVARY
                     LRSRGYDVTFVQNITDIEDKIIDKANQEGVSWQEIVDRFTDSFHERLQMLGIGLPDIE
                     PRATEHIPEMISLIEELVEKGYAYAPGNGDVYYSVESFPPYGALSHQRPEEMRETEKG
                     QTGFKRRPLDFALWKASKPGEPSWESPWGPGRPGWHIECSAMVRKHLPEGADIHGGGT
                     DIRFPHHENELAQSAAAHPDQPFVRAWAHHGMVTLTSGKMAKSVGNVLDVTRAVDLHG
                     RNAVRMWLLQSHYSQPIEYSEEILEEKRRSYERLMRLYLQISGSQSSSGLSDTLAARL
                     RESFDDAMQDDLNTPTALAALFEVAGRAGREISTHPEAADEFASLAEAIEEVMTIFGF
                     DLAQELSTWVEDVYIRYSEEPGAEVLNRVATRERARLEKDWATADLLREELYSDGWAV
                     EDTSEGPIISHR"
     gene            complement(3383..4693)
                     /locus_tag="VN178_09805"
     CDS             complement(3383..4693)
                     /locus_tag="VN178_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWS81304.1"
                     /translation="MPAGTALSGVRVRFAPSPTGLLHPGAARTALFNYLFAGYYGGDL
                     ILRIEDTDRERSRPEFERALIEHLEWLGLSFDEGPYRQSERGNLYEDAARRLTDAGLT
                     YESTDEPGRRALYFRPPVRSGSFVDELRGGVTFGGVEDFVIRKSDGTPSYNFAVVVDD
                     LELAVTHVIRGEEHLSNTGRQTLLYEALGATEPRFLHLGVILGPDGKKLSKRHGAASI
                     ADYRREGYMPEALVNYLALLGWNHPDGKVEFADLDEILREWDPSRLGSSPSTFDPDRL
                     RVFNANHIRRLPAEELRLRLDPFLEEPLPSGREPAALEAIREDMKTLSDAPRLLRGIT
                     GPVDPSAFVEALPDSSDEVFGHAATELEEREVGSVEEARSFVGRLRAWAKENGIKTRD
                     LLHPLRLALTGNNRGPEMAYLFAVLGAEEARERILRAREARLGA"
     gene            complement(4672..5151)
                     /gene="ispF"
                     /locus_tag="VN178_09810"
     CDS             complement(4672..5151)
                     /gene="ispF"
                     /locus_tag="VN178_09810"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWS81305.1"
                     /translation="MRVGLGVDVHAFAAPDEGRRLVLGGVEIPSDRGLLGHSDADVLA
                     HAIADALLGAAGLEDIGHYFPDTDERYRDADSIGLLREVRGLVGGYWEVANVDAVVIC
                     ERPKIRDHREAMRKNLAAALGTEESRIGVRGTTTERLGFPGRGEGIAAQAVCLLERR"
     gene            complement(5151..5837)
                     /gene="ispD"
                     /locus_tag="VN178_09815"
     CDS             complement(5151..5837)
                     /gene="ispD"
                     /locus_tag="VN178_09815"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWS81306.1"
                     /translation="MLAGGTGRRMGRPKQFIELLGMPALHYTLQAFEESPEVAKIYAV
                     GDRQRIEALVQAAGITKYAACAEPGEARSFSTRNGLLLCEEDPETIVLVHDGSRCLVT
                     PDLIKRVTGAARGAGEPDGVIPGIPVSDTIKVARGGTVTETLDRTNLYAVQTPQAFRL
                     GLLRRLHDSSEELLRAATDDASLVERHGGHVEVVAGEKTNIKLTSPEDLVFAEAILTV
                     RKGAASEVLP"
     gene            complement(5864..6949)
                     /gene="disA"
                     /locus_tag="VN178_09820"
     CDS             complement(5864..6949)
                     /gene="disA"
                     /locus_tag="VN178_09820"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HWS81307.1"
                     /translation="MSPQRRHLPAELARALELVAPGTDLRVAIDNVMRAHNGALIVLD
                     NPEKLESLGIISGGMKVEVEFAPMRLYELAKMDGAIVVSPDISAIHYANVQLNPDPGL
                     ESEETGMRHLAAHRTAQQTGSLVVAVSERRRVVSLYQGPYGPHVLEDIGVVLTKANSA
                     IATLEKFTRRLRKEARNLTVHEYDGAVTLREVVGAVGTFEYSARIAEEIEAYVRELGS
                     EGRLVEMQLEQAFHGVPEQYEALLRDYVEEGADNRAVREELRTLTAEQLSDSAEIARA
                     LGYDPEGQQEELFIKPRGYRQLERVPRLPRRVAESLIREFGSLKEILEATEEELDEVE
                     GIGQARARSIQRGLERQRSLETTGEIS"
     gene            complement(6946..>7736)
                     /locus_tag="VN178_09825"
     CDS             complement(6946..>7736)
                     /locus_tag="VN178_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HWS81308.1"
                     /translation="ECAARLMRLAKSEGIAVVLVGHVTKEGSIAGPRVLEHMVDTVLQ
                     FEGDRFQSFRVLRALKNRFGSTNEVGVFEMTDRGMVEVEDPSAFFLSRREEDVPPGVV
                     TVCLLEGTRPMLVEIESLVAPSPLANPRRIANGVDTGRVNMLCAVLSRRAGLSLHDQD
                     VYVNVTGGVRVEEPAADLGVALAIASAMRDRPVGKGTACFGEVGLTGDVRYVSGAQRR
                     TSEMAKMGFGRIIRPEGTSNGPPAREMSVVEVSTLEEAVAVALL"
BASE COUNT         1275 a         2592 c         2433 g         1436 t
ORIGIN      
        1 cacgtgccgc agaacgacgc cgccgacaag ctctcgcggc tgctcgaagc gcgcatgagc
       61 gaggaagttg ggtgcgaaat gggtgcgaaa tcggggtccg gcgaaagtgg caagggcgcc
      121 gaaagtgccg atttacaggg agaaaaaggt gccgggggag ggactcgaac ccccgacacg
      181 cggattatga ttccgctgct ctaaccgact gagctacacc gccacgccta tgttcgtaga
      241 ccgagtatac cccggtccca gagcccccga gcggacttga accgctgacc ccttccttac
      301 catggaagtg ctctaccgac tgagctacag gggcatattc cgtctgtgtg ggggcgggag
      361 gattcgaacc tccgtaggcg aagccggcag atttacagtc tgctcccttt ggccgctcgg
      421 gcacaccccc ggccacgccc ccgcagcact ctgtgcggag cgcccgaata tggtagcaaa
      481 cgctaccgac ttttcaacag atcgagaagg gaccgcgcag acctccgatc cagagcccgg
      541 gaagggattc gaacccccga ccggccgctt acaaggcggc tgctctacct ctgagctacc
      601 cgggcattag agccttcagc tatcagcagt cagccttcag cttctccagc tctcgacaca
      661 ctcatcgcca cacgagatgc gtcgccaaca ttctaacagg cggtctccgt acaacggcaa
      721 cgaaagcgta ggaatctcgg ccaacaacct tcttgctgaa ggctgatagc tgaaggctga
      781 cagctatttc ccgcgccggc gtatctcctc taacttacgc aaggtctcta gcgagagtcg
      841 gtctgctata cgggtcttgc gccggacttc cgggctgcgg gatatggccg gaagttcgtc
      901 gagtaggttt gcgaactcgc gcggggtggc gcgtgccgcc ccgcgggcga gggccgtgcg
      961 ttgtagggcg aagtcggccg tgtagattgt ggccgagcca tccagtctgg agagcaggcg
     1021 ttcgatcacg tcgtcagccg actcccccgc ggcgctgtag atcacgcgca cagccccgcc
     1081 ggcccgcgtc tcgactctct cgggctcggg actccggtgc gcgtcgaaga cgacaatgag
     1141 gggatttccg gtccaccccg ccgccttgag ggcgtcgttt atcaggaggt cgcgggcggc
     1201 gtcgagggtc cctgccgtcc cgtagcggtc gagggcgccg atgaggttgt agccgtccag
     1261 gatcagggcc acccgcgcct cctcctggcc tcgtagagca agaccgccga agcgacggag
     1321 acgttgaggg aatcgacggc cccaagcatg ggaatggaga ccgtcccgtc gcacccttcg
     1381 cgcacgagcc gccgcactcc ccgcccctcg ctgccgagca cgaacgccac ggccccggcg
     1441 aggtcgcgtc ccgtgtatgg cgcaccgccg gcctcggctg cgtagaccca gaggcccgca
     1501 cccttcatcg tgtctatggc ccgcctgagg ttcgtcaccc tggctacccg cacgtgctcg
     1561 ctcgcgcccg cgctcgactt gacggcggac gccgtcaccc ctacggccct gtccttcggg
     1621 atgacgaccc cgcaggcccc ggccccgtcc gcggcccgca agacggcccc gaggttgcgc
     1681 gggtcggtga ggccgtcgag gacgaggacg agaggctcgg gggaggaaag gatctccgcg
     1741 agtccggaat aaggataagg ttcgacgcgg gccactacgc cctggtgaac tccgcccctg
     1801 gcaagctcct ctacctgctg cctggatgcg cgtttcactt ctacgccacg cgcactggcg
     1861 gcctctgcga cctcttcgtt ccctaccgcg tcgatgacct ggaagacctt tcgacgcccg
     1921 ctccccagag cctcgaccac gggccttatg ccgtagatgg tctcccgttt caattaccgg
     1981 tggctgatga tcggaccttc agaagtatcc tccacggccc acccatcgga gtaaagttcc
     2041 tcgcgcagca ggtcggcggt ggcccaatcc ttctcgagtc tcgctctctc tctcgtggcc
     2101 acgcgattca gaacctccgc gccaggctcc tcggagtacc tgatgtacac gtcttctacc
     2161 caggtagata gctcctgggc caggtcgaaa ccgaagatgg tcatgacctc ctctatggcc
     2221 tctgcgagag atgcgaactc gtccgcggct tcggggtgag tggagatctc acgccccgcc
     2281 cttcccgcga cctcgaagag cgccgccagg gccgtcgggg tgttcaggtc gtcttgcata
     2341 gcatcgtcga agctctcacg tagcctggcg gcgagtgtgt cagagaggcc cgaggaactt
     2401 tgcgaaccag agatctggag gtaaaggcgc atgagacgct cgtaggagcg gcgcttctct
     2461 tcgaggatct cctccgagta ctcgatgggc tgggagtaat ggctctgcag gagccacatc
     2521 cgcacggcgt tgcggccgtg gaggtcaacg gcccgcgtca cgtcgagcac gttcccgaca
     2581 gatttagcca tctttccgct cgtgagcgta accatgccgt gatgggccca cgcacgaacg
     2641 aacggttggt cagggtgggc cgcagcgctc tgggcgagct cgttctcgtg gtgcggaaaa
     2701 cgtatgtccg ttccgccgcc gtggatgtcg gccccctcgg gcaggtgctt gcgcaccatc
     2761 gcggagcact cgatgtgcca gccggggcgt ccgggtcccc acgggctctc ccaggaaggc
     2821 tcaccgggtt tggaggcctt ccacagcgcg aagtccagtg gtctccgctt gaaaccggtc
     2881 tggcccttct ccgtctcgcg catctcttcg ggacgctggt ggctcagggc tccgtaaggc
     2941 gggaagctct cgaccgagta gtagacgtca ccgttaccag gggcgtacgc gtagcccttc
     3001 tctacgagct cttcgatcag ggagatcatc tcggggatgt gctcggtcgc gcgtggctcg
     3061 atgtcgggca gtcctatgcc tagcatctga agccgctcgt ggaaggagtc cgtgaagcgg
     3121 tctacgatct cctgccagga gactccttcc tggttggcct tgtctatgat cttgtcctct
     3181 atgtccgtga tgttctggac gaacgtcacg tcgtaacccc ggctcctcag gtacctggcg
     3241 accacatccc agaacaatgg cgcccgcgcg ttgccgacgt ggatgtgatc gtagaccgta
     3301 ggaccgcaga tgtagaggcc gagaccacca ctctccccca cctcgaccgt cctgccgctc
     3361 atgctgtctt ttacgagcaa actcatgccc ctagtctagc ctctctagcc cgtagtatcc
     3421 tctccctggc ctcttcggca cccaagacag cgaagaggta ggccatctcg ggacccctgt
     3481 tgttgccagt gagcgctagc ctgagaggat gcaggagatc cctggtcttt atgccgttct
     3541 ctttcgccca cgcacgcagt ctgccaacga agctacgcgc ctcttccacg ctcccgacct
     3601 cgcgttcttc cagttcggtg gcggcgtgcc cgaagacttc gtcgctggag tcgggcaacg
     3661 cctcgacgaa cgcggacggg tccacggggc ccgtgatccc acgcagcagg cgtggcgcgt
     3721 cggagagggt cttcatgtct tcccttatgg cctcgagtgc cgcgggctcc cgcccgctcg
     3781 gcagcggctc ttcgaggaaa gggtccaggc gcaggcgaag ctcctcagcg ggcaggcgcc
     3841 ggatgtgatt ggcgttgaag acccgtagac ggtccggatc gaaagtcgac gggctcgacc
     3901 cgagacgcga aggatcccat tcgcgcaaga tctcatcgag gtctgcaaac tctacttttc
     3961 catccgggtg gttccagccg agcagggcga ggtagttgac cagcgcttcg ggcatgtatc
     4021 cttccctgcg gtagtcggcg atgctcgcgg cgccgtggcg tttggagagc ttcttgccgt
     4081 cgggaccgag gatgacgccc aggtggagaa acctgggctc ggtcgcgccg agcgcctcgt
     4141 agagcagggt ctggcggccg gtattggaga ggtgttcttc accccggatc acatgtgtaa
     4201 ctgccagctc cagatcgtct acgaccacgg cgaagttgta cgaaggggtg ccgtcggact
     4261 ttcggatcac gaagtcctcc acgccgccga acgtcacccc gcccctcaac tcatcgacga
     4321 agctaccgct acgcacgggc gggcggaagt agagcgcgcg ccgtcccggc tcgtccgtgc
     4381 tctcgtaggt gagcccggca tccgtcagac gccgggcggc gtcctcgtag aggttgccgc
     4441 gttcactctg acggtaagga ccctcgtcga atgacaatcc gagccattcg aggtgttcga
     4501 taagcgcgcg ctcgaactcg ggtctgctcc gctccctgtc ggtgtcctcg atacggagga
     4561 tcaggtctcc cccgtaatac ccggcgaaga ggtagttgaa gagcgcggtg cgggccgcgc
     4621 cggggtggag gagccccgtc gggctcggtg cgaagcggac cctgacgccg ctcaacgccg
     4681 ttccagcagg cacaccgcct gggcggctat gccctcgccc ctgcccggaa agccgagacg
     4741 ctcggtcgtg gtgccgcgaa cgcctatgcg ggactcttcc gttccgaggg cggcggcgag
     4801 attcttccgc atggcctccc tgtggtccct gatcttgggc cgctcgcaga tgacgacggc
     4861 gtccacgttg gcaacctccc agtaccctcc aacgagaccg cggacctcgc gcagcaaacc
     4921 gatagagtcc gcgtccctgt aacgctcgtc ggtgtcgggg aagtagtgtc cgatgtcttc
     4981 gaggcccgcc gccccgagaa gggcgtccgc aatagcgtgg gccagcacgt cggcgtcgga
     5041 gtggccgagg aggcccctgt cggaggggat ctcgaccccc ccgagtacga gcctgcggcc
     5101 ttcatcgggc gcggcgaagg cgtggacgtc gacgcccagc ccgacccgca tcaaggcagg
     5161 acctcactgg cagcaccctt gcgtacggtg agtatggcct cggcgaagac cagatcctcg
     5221 ggagaggtca gcttgatgtt ggtcttctct ccggcgacga cctcgacatg ccctccatgc
     5281 cgctcgacca gagaggcgtc atcggtcgcg gcacggagga gttcttcgga cgagtcgtgg
     5341 aggcggcgca gcaggccgag gcggaacgct tgcggcgtct gaaccgcata caggttcgtc
     5401 ctgtccaggg tctcggtgac cgtgcctccc ctggcgacct tgatagtgtc agagaccggt
     5461 atccctggga tgacgccgtc aggctcgcca gctccacgtg ccgcccctgt gacgcgcttt
     5521 atgaggtcgg gtgtgaccag gcagcgagac ccgtcgtgca ccagcacgat ggtctccggg
     5581 tcttcctcgc agaggagcaa accgttcctg gtcgagaagg agcgggcttc gcctggctcg
     5641 gcgcaggcag cgtacttggt aatgccggcc gcctgcacga gagcttcgat cctctgcctg
     5701 tcgcctacgg cgtagatttt ggcgacctcg ggggactctt cgaaggcctg cagcgtgtag
     5761 tggagggccg gcatgccgag gagttcgatg aactgcttgg ggcggcccat cctgcggcct
     5821 gtcccgccgg cgagcactag cgcgatagct ggtccctggt cttctatgag atctcgcccg
     5881 tggtctccag gctgcgctga cgttcgaggc cgcgttggat ggagcgggcg cgggcctggc
     5941 cgatcccctc tacctcgtcg agctcctcct ccgttgcttc gaggatctct ttgagggagc
     6001 cgaactcccg gatgaggctc tcggcgaccc tgcgcggcag gcgtggcacc cgctctagtt
     6061 ggcggtatcc gcggggtttg atgaacaact cctcctgctg gccttccggg tcgtagccga
     6121 gggcgcgcgc gatctcggcc gagtcggaga gttgctcggc cgtgagggtc cttagctctt
     6181 ccctaacggc cctgttgtcc gccccttctt cgacgtagtc cctcagcaag gcctcgtatt
     6241 gctcggggac cccgtggaag gcctgctcca gctgcatctc gacgagccgg ccctcgctac
     6301 ccagctccct cacgtaagcc tctatctcct cggctatcct ggccgagtac tcgaaggttc
     6361 cgacggcgcc gacgacctcc cgcagcgtca cggccccgtc gtactcgtgt acggtcaggt
     6421 tccgcgcctc cttcctcagt ctcctggtga acttctcgag cgtcgcgata gccgaattcg
     6481 ccttggtcag gaccaccccg atatcctcga ggacgtgcgg cccgtaggga ccctggtaga
     6541 ggctcaccac cctcctgcgc tcggagaccg ctacgacgag gctacccgtc tgctgcgccg
     6601 tgcggtgggc cgccaggtgg cgcattccgg tctcctcgga ttcgaggcca ggatcggggt
     6661 tgagctggac gttcgcgtag tggatggcgg agatatccgg cgagaccacg atagcgccgt
     6721 ccatcttcgc cagctcgtag aggcgcatcg gcgcgaactc gacctcgacc ttcatcccgc
     6781 ccgagatgat gccgaggctt tcgagctttt cgggattgtc gaggacgatc agggccccgt
     6841 tgtgcgccct catgacgttg tctatggcca ccctgagatc ggtaccaggg gccacgagct
     6901 cgagggcgcg cgccaactcc gcgggcagat gccgccgctg gggactcata gcaacgccac
     6961 cgccacggct tcctccaagg tgcttacttc cacgacgctc atctctcttg ccggcgggcc
     7021 attcgacgta ccttccggcc tgattatacg accgaagccc atcttggcca tctcggaggt
     7081 ccggcgctgg gcgcccgaga cgtagcgcac gtcccccgtt agcccgacct cgccgaaaca
     7141 ggcagttccc ttgcccacgg gcctgtctcg catcgcagaa gcgatggcca gcgcgacccc
     7201 gaggtcggcc gcaggctctt cgacccgcac accgccggtg acgttgacgt acacgtcctg
     7261 atcgtgtagc gagagaccgg ccctcctgct caacaccgcg cagagcatat tgacccgccc
     7321 cgtgtccacc ccgttggcga tacgccgtgg gttggcgagc gggctcggtg cgacgaggct
     7381 ctctatctcg accagcatgg gccgcgtccc ttccagaaga cagaccgtga cgacgccagg
     7441 cggcacgtcc tcctctctcc tggagaggaa gaacgcggaa gggtcttcga cctcgaccat
     7501 tccgcggtcg gtcatctcga agacacccac ctcgttcgtc gacccgaagc ggttcttgag
     7561 ggcccgcaag acccgaaagc tctggaagcg gtccccctcg aactggagca ccgtgtcgac
     7621 catatgctcg aggacgcgcg gtcccgcgat ggacccctct tttgtcacgt gccctacgag
     7681 gaccacagcg atgccctcgc tcttggcgag ccgcatgagc ctggccgcgc actcgc
//
LOCUS       DATMVF010000198         7127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_20696, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000198 DATMVF010000000
VERSION     DATMVF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20696"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VN178_09830"
     CDS             complement(<1..503)
                     /locus_tag="VN178_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81309.1"
                     /translation="MEGVDLLGKTAFVIGASSGIGLATANLFADAGVTVHAAARRREA
                     IEEGAGGRNVTAYALDISDKGAVWRTVEEVGESDGLDVLVVAAGMNFPERRLEQLTAE
                     GWDAMISVNLSGAFYAIRAALPYLRASQGLVVLISSVSGRWPDASGPAYQASKAGMVE
                     LAHAAGF"
     gene            complement(503..1528)
                     /locus_tag="VN178_09835"
     CDS             complement(503..1528)
                     /locus_tag="VN178_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HWS81310.1"
                     /translation="MKGIVWHGPEQMSVEEVPEPAVEPGTVVVRPTAAGICGSEIEGY
                     LGRMGNRTPPLVMGHEFAGTVTEVGEGADQDLLGRLVAVNPLSSDGTCRLCQAGLTNL
                     CPNRSLVGIHSPGGFAEYTLAPAANVYPLPDGVEARSGALAEPLANGVHAARLGLADG
                     PVEHAVVIGAGTIGLMCLQAAVLDGIPEVHAVEPVEARREQALALGAGAVHESAEEAR
                     GALEEPTEGLGADLVIDAVGAEVTRRMALDLLRPGGRVVYVGLHDDDTTLGFHDVVRG
                     QLDLQGSYAYTAEDYEQALDWLVEGKAGIGELPPVLPLEEGPEAFAGLVRGPSAQIKV
                     FLAESGQ"
     gene            complement(1595..2311)
                     /locus_tag="VN178_09840"
     CDS             complement(1595..2311)
                     /locus_tag="VN178_09840"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWS81311.1"
                     /translation="MDQGTVGNASPFDGYEWVDLSHTLEEDIPAYSTHARFGKTLYES
                     YEYGDAALHYGLTMSEHTGTHMDAPLHFISEGPAHYGTDEIPLDRLAGRAATIEATDL
                     GADDLLGAGRIEAWEEEHGPIEAKDRVLIRYGWDRRWTTGPEGRRFLEDWPGLSGEAA
                     EYLVGKGVSLVGCDTLAIDATVSTKNPAHYALLGSEVYIVENLKNLDRLPPFCHFLAL
                     PLKIRGGSGSPVRAVALVPR"
     gene            complement(2532..3539)
                     /locus_tag="VN178_09845"
     CDS             complement(2532..3539)
                     /locus_tag="VN178_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWS81312.1"
                     /translation="MDQERLIPWTGPGWFEQASAWVHAELELQDITVSGPVEQPHSRP
                     WSTVLRVPTIEGNIYFKAVSPVDPYEPPLLGALARWRPERVPRLLSVDAGRGWVLMRD
                     AGQRLRELIRPTRDTRPWLPVLPYYAEMQIELAQRVPELLALGVPDRRLPDLPGLYEP
                     LLADVEVLRIDRRPGLTRQQYERLLEMSWRVAELCERLDDYRIPETINHGDLNDGNVF
                     VGEGHSIFIDWGDACVSHPFYSLRTVLVSAEISLGLEENSPELRPLRDAFLEPWTRYG
                     SRKDLLAALDLAGRLASINGALTWHRLVSKLEGATREEYAEPVPALLQEFLDAETTGS
                     E"
     gene            complement(3662..3991)
                     /locus_tag="VN178_09850"
     CDS             complement(3662..3991)
                     /locus_tag="VN178_09850"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS81313.1"
                     /translation="MNPYALWIQDLPQVDTPFEGLEGRMISGPDGQAVFFRAAERFEV
                     PPHSHGAQWGVVVTGLLHLTIDGEEATYEPGETYDIPGGAEHSAILEAGTCVIDVFQD
                     PDRYSPK"
     gene            4077..4844
                     /locus_tag="VN178_09855"
     CDS             4077..4844
                     /locus_tag="VN178_09855"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWS81314.1"
                     /translation="MERLEQKRAIVTGAGSGIGRAIALRFAGEGARVVVSDVDGEAAA
                     SVASEAGDGASGETLVSKTDVTDSSEVEALVGTAVEAWGGLDVMVNNAGIGVAGTTTN
                     TSEENYERVMDVCLRGTFLGMKHAIPAIGDSGGGSVINLSSVAALVGIADRAVYSAAK
                     GAILAITRAAAIDHVEEGVRVNCIAPGTVDTPWIGRITAGYDDPDEARQNMQARQPHG
                     RFVAPEEIAAMAAYLASDESASVIGACMIVDGGITAR"
     gene            4841..5494
                     /locus_tag="VN178_09860"
     CDS             4841..5494
                     /locus_tag="VN178_09860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS81315.1"
                     /translation="MNEPHKGTVESGYDQMAEQYLATRDPEDPLALRALADLASLLPK
                     EAAVLDLGCGAGVPVTRWLADRGFAVTGVDVSAKQLELARTYVPEGTFIKADMTEVDF
                     GLESFDAVVAFHSIIHVPRTEHPALLERIHRWLRTGGALLATMAVADYEGRDEDWEGW
                     GAPMVWSHYDRNANVAMLRDAGFEIRYAEPRTGGGTGDETETWLWVLASKKTREGTT"
     gene            5491..6801
                     /locus_tag="VN178_09865"
     CDS             5491..6801
                     /locus_tag="VN178_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HWS81316.1"
                     /translation="MTTITRVVARDIRFPTSLDLSGSDAMNEAPDYSAAYVVLETDGG
                     LEGHGFTFTIGRGNELCVAAIRALAPLVEGRTLESFTRDMGAFWRHVTGDSQLRWVGP
                     EKGVIHLATAAIVNAVWDLYAKSERKPLWRLLSDMSPEELISCIDFRYITDAITREEA
                     LEILRRNEASKPDREAEMLEDGFPAYTTSAGWLGYSDERLRSLVREAVGKGWTHFKMK
                     VGGDIEDDVRRAALIRDEIGPDRTLMMDANQVWEVDQAIENMERLAKFEPYWIEEPTS
                     PDDVLGHARIARAVWPVGVATGEHCQNRVLFKQLLQADAIRFCQIDSCRLGGVNEVIA
                     VLLMAAKFGVPVCPHAGGVGLCEYVQHLSIFDYICVGASLEDRILEYVDHLHEHFVDP
                     VEIQGGRYVPPQAPGYSAEMRPGSLNEFEFPTGGAWAAKERSIP"
     gene            6798..>7127
                     /locus_tag="VN178_09870"
     CDS             6798..>7127
                     /locus_tag="VN178_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81317.1"
                     /translation="MNGRVAAVLGVGPGLGAAVARRFAGGGFAVAMMARREESLAEIQ
                     QDIEGDGGTALPVSADATDPESVAAAFGRIRSELGDPEVLVYNAGAFQMGGVLEISPA
                     QFDECFRA"
BASE COUNT         1264 a         2365 c         2312 g         1186 t
ORIGIN      
        1 tcgaaaccgg cggcgtgcgc cagctcgacc atcccggcct tcgaagcctg gtaggccggg
       61 cccgaggcgt cgggccagcg tcccgagacg ctgctgatca ggacgaccag tccctgcgaa
      121 gcccgcagat agggcaatgc cgccctgatg gcgtagaaag cgccgctgag gttcaccgag
      181 atcatggcgt cccatccctc ggccgtaagc tgctccagcc tccgctcggg gaagttcatt
      241 ccggcggcga cgacgaggac gtctagaccg tctgattccc ccacctcttc gacggtccgc
      301 cagacggccc ctttgtccga gatgtcgagc gcgtaagccg taacgttgcg tcctcctgcg
      361 ccctcttcta tggcctcgcg gcgccgggca gcggcgtgca cggtgacccc cgcgtctgcg
      421 aagaggttgg ccgtggcgag cccgataccg ctgctcgccc cgatgacgaa cgccgtcttc
      481 cccaaaaggt ccacgccttc cactactgcc ccgactccgc gaggaagacc tttatctgtg
      541 ccgaggggcc acgcaccaac ccggcgaacg cctcggggcc ctcctccagc ggtaagacgg
      601 gcggtagctc cccgataccg gccttgccct cgacgagcca gtcgagcgcc tgctcgtaat
      661 cctccgccgt gtacgcgtac gagccttgca ggtcgagctg gccccgtacg acgtcgtgga
      721 acccgagggt cgtgtcgtcg tcgtgcaacc ctacgtacac gacccgtccg ccgggccgca
      781 ggagatccag ggccatcctg cgtgtgacct cggcgccgac ggcatcgatg acgaggtcgg
      841 caccgagccc ttcggtgggc tcctccagcg ccccgcgcgc ctcctcagcg gactcgtgga
      901 ccgcgcccgc tccgagggcg agcgcctgct cgcgacgtgc ttccaccggc tcgacggcgt
      961 gtacttcggg gatgccgtcc agcaccgcgg cctgcaagca catcaggcct atggtaccgg
     1021 ccccgatcac gacggcgtgc tctacggggc catcagcgag cccgagcctc gcggcgtgga
     1081 cgccgttggc gagaggctcg gcgagggcgc ccgaacgcgc ctcgacgcca tcggggagcg
     1141 ggtagacgtt cgccgctggc gcgagcgtgt attcggcgaa tccgccaggg gagtggatgc
     1201 cgaccaggct gcgattcgga catagattgg taagccctgc ctggcacagg cggcacgtcc
     1261 cgtcggagga gagcgggttc acggccacca gtcgaccgag gagatcctgg tctgcccctt
     1321 caccgacctc cgtgacggtg ccggcgaact cgtggcccat cacgaggggc ggagtgcgat
     1381 tgcccatcct gccgaggtag ccttcgatct ccgagccaca gatccccgct gccgttggac
     1441 gaacgacgac ggtcccaggc tcgacggccg gctcgggtac ctcctccacg gacatctgct
     1501 caggcccgtg ccacacgatc cccttcatac ttccaccttc cctttgcgaa cgtctcagat
     1561 agcctatctt actcccgagc cgctcgtgct gcgatcagcg cggcacgagc gcgacggccc
     1621 taactggcga gcccgagccg ccgcggatct tcagcggcaa cgcgagaaag tgacagaacg
     1681 gcggcaaacg atccaggttc ttcaggttct cgacgatgta cacctcactg ccaagcagcg
     1741 cgtagtgggc cgggttcttc gtagaaacgg tggcgtcgat cgcgagcgta tcgcacccca
     1801 cgagcgagac gccctttccg accaggtact ccgcagcctc tccagagagg ccgggccagt
     1861 cttcgaggaa gcgtcgtcct tctgggccgg tcgtccacct gcggtcccat ccgtagcgga
     1921 tcaagaccct gtctttcgcc tcgatggggc cgtgctcctc ctcccacgcc tcgatgcgcc
     1981 cggcgccaag cagatcgtct gctccgaggt ccgtggcttc gatggtagcg gcgcgtcccg
     2041 cgaggcggtc gagggggatc tcgtccgtcc cgtaatgggc cggaccctcg gagatgaaat
     2101 gaagcggcgc gtccatgtgg gtccccgtgt gttcgctcat cgtgagacca tagtggaggg
     2161 cagcgtcacc atattcatac gactcgtaga gggttttgcc gaagcgggcg tgcgtagagt
     2221 aggcggggat atcctcctcc agcgtatggg agagatctac ccactcgtaa ccgtcgaacg
     2281 gtgaagcatt tccgaccgtg ccctgatcca atcccctaca cccctctggc acagcgtcta
     2341 tgatggacgc ttacatcacg agggattccg gccatgtttc tcctctcccg acctccgcgc
     2401 ggaccgcaca gaacttcctt cagagcgccg tcgggatcac gcggatccga cagcggacac
     2461 gacccgcgca ctatactccg gcacgctcgc ccaaggcaac cgccgcgcat gcggcgccaa
     2521 cggggaggtt tctattcgga accggtcgtc tcggcgtcga ggaactcctg cagcaacgcc
     2581 ggcacgggct cggcgtattc ctctctcgtt gctccttcca gcttcgacac gaggcggtgc
     2641 caggtcagcg cgccgttgat gctggccagc cggccggcca gatccagggc cgccaacaaa
     2701 tccttgcgcg acccgtagcg cgtccacggc tccaggaatg catcgcgcag gggcctgagc
     2761 tcgggggagt tctcttccag cccgaggctg atctccgcgc tcaccagcac cgtgcgcagc
     2821 gagtagaacg ggtgcgagac gcacgcgtcg ccccagtcga tgaagatgga gtgcccttcg
     2881 ccgacgaaga cgttgccgtc gttgaggtcg ccgtggttga tcgtctccgg gatgcgatag
     2941 tcgtccaacc gctcgcacag ctcggccacc cgccaggaca tctcgagtag ccgctcgtac
     3001 tgctggcgcg tcagccccgg acgtcggtct atccgcagga cctccacgtc ggccagcagt
     3061 ggctcgtaca ggccaggcag gtccggcaag cgccgatctg gcacgccgag cgccaatagc
     3121 tcggggaccc gttgcgccag ctcgatctgc atctcggcat agtacggcag tacaggaagc
     3181 cagggacgcg tgtccctggt ggggcggatg agctcgcgca gcctctgacc ggcgtcacgc
     3241 atcagcaccc agccccgtcc cgcatctacc gacaagagac gcgggacgcg ctctggccgc
     3301 cagcgcgcca gcgccccaag cagtggcggc tcgtacggat ctaccggcga gaccgccttg
     3361 aagtagatgt tgccttctat cgtcggtacg cgcagcactg tagaccaggg acgtgagtgc
     3421 ggctgctcta cagggccgct cacggtgatg tcctggagct ctagctccgc atggacccag
     3481 gcgcttgcct gctcgaacca gccaggtcct gtccacggga ttagtcgttc ctgatccata
     3541 accgcctcgt ccctgcttcg gagccagcgc ccgatcttct agcgcaatac ggagaacgct
     3601 agggtgccgt cgagcagagg gagtcccgat ctacgccagc ctgtacctta cgcgagggtt
     3661 tctacttcgg gctgtagcgg tcggggtcct ggaagacgtc tatgacgcag gtcccggctt
     3721 cgaggatggc tgaatgttcg gcaccgccag ggatgtcgta ggtctcgcca ggctcgtagg
     3781 tcgcctcctc tccgtctatg gtcaggtgga gcaggccggt cactactacg ccccattgcg
     3841 cgccgtggga gtggggaggg acttcgaacc tctccgcggc cctgaagaag accgcctgtc
     3901 cgtcgggacc ggagatcatt cgcccctcca aaccctcgaa gggcgtgtct acctgcggga
     3961 ggtcctggat ccaaagcgca taagggttca aagcgactcc tttccctgcg aacggcgcgt
     4021 ttgcgccgcc gctcagggcg tcattatact gaccgtttca actatccgga ggtgagatgg
     4081 agcgcctgga acagaagaga gcaatagtaa ccggcgcggg ctctggcatc ggccgtgcca
     4141 tcgccctcag gttcgccggg gagggggccc gcgtggtcgt ctcggacgtg gacggagagg
     4201 cggcagcgag cgtggcgtcc gaggcaggtg acggagccag tggcgagacc ctcgtcagca
     4261 agactgacgt caccgactcc agcgaggtcg aggcgctggt agggaccgcg gtcgaggcgt
     4321 ggggcggcct cgacgttatg gtcaacaacg cggggatagg cgtggccggc accactacca
     4381 atacctcgga agagaactac gagagggtga tggacgtgtg cctcaggggt acgttcctcg
     4441 ggatgaagca cgccatcccc gccatagggg attcaggggg cgggtcggtt atcaatctgt
     4501 cttcggtggc cgcgctcgtg gggatagccg acagggccgt ctacagcgcg gcgaagggcg
     4561 cgatcctcgc catcacccgc gccgcagcca tcgaccatgt agaagaaggc gtgagggtca
     4621 attgcatcgc gccgggcacc gtggatacgc cctggatcgg ccgcatcacc gcaggctacg
     4681 acgaccccga cgaagcgcgc caaaacatgc aagcccgcca gccccacggc cggttcgtcg
     4741 cgccagagga gatcgccgcg atggcggcct acctcgcctc ggacgaatcg gcgtcagtga
     4801 tcggggcgtg catgatcgtt gacgggggca taacagcaag gtgaacgaac cccacaaggg
     4861 aacggtcgag tccggctacg accagatggc cgaacagtat ctggccacca gggatcccga
     4921 agacccactc gccctgaggg cccttgcgga tctggcctca ctcttgccga aggaagccgc
     4981 tgtgctggat ctcggttgcg gcgccggcgt gccggttaca cgctggctcg ccgacagagg
     5041 gttcgccgtt acgggggtcg acgtctccgc aaagcaactg gagctggccc gcacatacgt
     5101 accggaaggc accttcatca aagcggacat gaccgaagtg gatttcggac tcgagtcctt
     5161 cgacgcagtg gtcgcattcc actctatcat ccacgtgccc cgcaccgaac atcccgcgct
     5221 tctggagcgc atccaccgct ggctgaggac gggtggcgcg ctccttgcga ccatggccgt
     5281 agcggactac gagggccgtg acgaagactg ggaaggatgg ggggcgccga tggtgtggag
     5341 ccactacgac aggaacgcca acgtggcgat gctgcgggac gccggcttcg agataaggta
     5401 cgccgagcca cgcacaggcg ggggcacggg cgacgagacg gagacctggc tctgggttct
     5461 tgccagcaag aagacgagag aaggtacgac gtgaccacca taaccagggt agtagcccgc
     5521 gacatccgct tcccgacgtc gctggatctc tccggctccg acgccatgaa cgaggccccc
     5581 gactactcgg ctgcctacgt cgtgctcgag acggacggcg ggctggaggg ccacggcttc
     5641 acctttacca tcggacgcgg caacgagctg tgcgtcgccg ccataagagc gctcgccccg
     5701 ctagtcgagg ggagaaccct cgagtcgttc acccgcgaca tgggcgcttt ctggcgtcac
     5761 gtcacggggg acagccagct caggtgggta gggcctgaga agggtgtgat ccacctcgcc
     5821 acagccgcca tcgtcaatgc cgtctgggat ctctacgcta aaagcgagcg caaaccactg
     5881 tggagactcc tctcggacat gagtccggaa gagctgatct cttgcattga cttccgctac
     5941 ataaccgacg cgatcacccg tgaagaagct ctcgagatcc tgcgccgaaa cgaagcgtcg
     6001 aaaccggacc gtgaggcgga gatgctggag gacggtttcc ccgcctacac cacgtccgcc
     6061 gggtggctcg ggtattcgga cgagaggctg cgctcgctcg tgcgtgaggc ggtcgggaag
     6121 ggctggacgc acttcaagat gaaggtcggc ggggatatcg aggacgacgt gcggcgtgcc
     6181 gccctgatcc gggacgagat cggacccgac cgcaccttga tgatggacgc caaccaggtc
     6241 tgggaagtgg atcaggcaat cgaaaacatg gagcgtctcg cgaaattcga accgtactgg
     6301 atcgaggaac ccacgagccc cgacgacgtc ctgggccacg cgcgcatagc cagggccgtc
     6361 tggccggtcg gcgtcgccac gggtgagcac tgccagaacc gcgtcctttt caagcagctc
     6421 ttgcaggctg acgccatacg tttctgccag atagactcct gccgcctcgg cggcgtcaac
     6481 gaggtcatcg ccgtgctcct gatggcggcg aagttcggcg tacccgtctg cccccacgca
     6541 ggcggcgtcg gactgtgcga gtacgtccag cacctctcca tcttcgacta catctgtgtc
     6601 ggcgcttcgc tcgaggaccg gatcctggag tatgtggacc acctccacga acacttcgtc
     6661 gaccccgtgg agatccaggg cggccgctac gtcccgccgc aagcccccgg ctactccgcc
     6721 gagatgagac ccgggtccct gaacgagttc gagttcccaa cgggaggcgc ctgggccgcg
     6781 aaagagagga gcatcccatg aacggaagag ttgcggcggt actcggggtc ggtccaggac
     6841 taggggctgc ggtcgcccgc cgcttcgccg gcggaggctt cgctgtcgcg atgatggccc
     6901 gccgggaaga gagccttgcg gagatacaac aggacatcga gggcgacggc ggtacggcgc
     6961 tccctgtctc cgccgacgcc acggacccgg aatcggtcgc cgctgccttt gggcgaatac
     7021 gttcggagct cggagatccc gaagtgctgg tctacaacgc cggcgcgttc cagatgggtg
     7081 gcgttctgga gatttcgccg gcgcagttcg acgaatgctt ccgggca
//
LOCUS       DATMVF010000199        22204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_620734, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000199 DATMVF010000000
VERSION     DATMVF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22204
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620734"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VN178_09875"
     CDS             complement(<1..318)
                     /locus_tag="VN178_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HWS81318.1"
                     /translation="MDKSKAIDAAISQIERQFGKGSIMRMGDEERQSVASIPTGALAL
                     DLALGVGGVPRGRIVEIFGPESSGKTTLGLHIIAEAQKAGGLAAFIDAEHAIDPTYAQ
                     AIGV"
     gene            complement(423..1301)
                     /locus_tag="VN178_09880"
     CDS             complement(423..1301)
                     /locus_tag="VN178_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWS81319.1"
                     /translation="MEDWRHTQDPAEGDQSRGEIGRLLEHKRKERGLSLEEVEQATKI
                     RKRYLTGLEREDYAVLPDAVYARGFLKTYANYLGLDGEALSQRLKNSTKPQRERGSDS
                     DPTPESDFEKPLISPSGLRAADKRKVSSSAVATVMVAVLALAAVIGALYFVGRGVQTT
                     KENDPAPGETPPRQEQQNVAGTEKDPKDAVESKGTSEEKPVVAKQDAPPDTLRVVVDV
                     RERPSWILIRTDGNRAYKQVAQPGFSETFEAEHRLFIKSGDAGAVWVEINGQDAGALG
                     DSGEIVARRYTLKSAS"
     gene            complement(1327..3597)
                     /locus_tag="VN178_09885"
     CDS             complement(1327..3597)
                     /locus_tag="VN178_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HWS81320.1"
                     /translation="MATGKRAPKKTAKKRTSGKGKSKRKGTSTLTRVREGLTGALSRE
                     AVGVFLLAVGLFFGAAFASGRGSFLGDAGSFVSTQLLGSVGLALAPLAVVAGLLLLLG
                     RLRGRLVAGAALLLLAGAASFAATLAPRHQFSARHYPEAGGVLGSGIYAGVEWAAGAI
                     GAALTIALLYVVGLSLLTGVSFAAALGALRVVGEKVAGLGRGLWEKRSGRPEPPAKRG
                     RTDPAKVRETAEPDAWDEDEGVSAEPDSGPSEGAKTREFEVVLPDRGPELGFSAQREL
                     APGEYTPPPFSMLSLGQGDPEHDAEGTSRRLTLALSDLGVEAHVVRAVVGPRVTRYEL
                     RLGSGVKVGKVRNLQQDIAYALAATEVRILAPIPGKSAVGVEVPNTRPAKVTLGDVFR
                     AYPDANDWTLPVGLGKDISGRAVFFDLSEMPHLLVAGTTGSGKSVMLNGLLTSLLLTT
                     DPRQVKMVLIDPKRVELSQFGRVPHLITPVVTDVKKAANALGWAVAEMERRYEVLEKN
                     GLRSVEGYNDRAEVQIPYVVVVIDELADLMMQAGAKVEDAIIRLAQKARAVGIHIVVA
                     TQRPSVDVITGMIKANVPSRIAFAVSSQVDSRVILDSPGAEALLGMGDMLYKPVSAAR
                     PSRVQGAFISEAEVDRVVSATVEAASGSAEARFVEEVTEPRSVKEENDEPDDDLLPEA
                     ASFVVSTQQASVSAVQRRFRVGYSRAGRIIDALERKGVVGPYEGSKSRAVVASTMDLE
                     TLFGEDEGRSERAEGW"
     gene            complement(3663..5306)
                     /locus_tag="VN178_09890"
     CDS             complement(3663..5306)
                     /locus_tag="VN178_09890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWS81321.1"
                     /translation="MQVVPLGGLGEIGKNMTAVRQDSGMILVDAGMSFPDEEMPGIDL
                     VLPDFTYLREHANELKGIVLTHGHEDHVGALPYLLREFNVPVYATKLTLGLVRSKLQE
                     FGIKRGDLREIKAGDRQNLGGFGLEFIEVNHSIPGAVAIAIRTNAGVIVFSGDYKIDL
                     TPIEGSPMDLGRLAELGKEGVLAYFGDSTNSERPGYVPSERIVGDTLNEVFESAKGRI
                     IVASFASHIHRVSQVVEAAKKHDRLVGVTGRSMIRNVQIARELGYLDLPQEMMVDQRD
                     MGQIPDSDIVVLTTGSQGEPLAALSRIANGTHRTIEVGPEDTVVIAAHPIPGNERGVS
                     RTINNLMKRGAHVFYSPLMPVHVSGHAAQEEQKIVLSLLKPRYLVPVHGEFRHLRHHA
                     NTAMSLGIPEDNIFILENGDCLEFVDGDAHRTDNVAAGMFLVDAGAIAESSSGVMRER
                     QQLSEDGMFIVVARINAQNGQLLGPPDVISRGFVSQESANGLVDQSIEVVSQTLEQTA
                     SHRVTDWGELKNAIRKDLSRFLSDRTGKRPIILPLIVEV"
     gene            complement(5407..6663)
                     /locus_tag="VN178_09895"
     CDS             complement(5407..6663)
                     /locus_tag="VN178_09895"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWS81322.1"
                     /translation="MADPNIRRKDLPGNLRVFSEPLAEATSVALGLWIRAGSRDETDE
                     VAGISHLMEHMLFKGTPEMDALGIAQAFEGIGAQENAATGEEYTVLYARFLPEHLGRA
                     LDIMSDMVLRPTLVDLEREREVIVEEIKMYEDRPDQLADEYLSSLIFHADPLGRPIIG
                     SAETVRGVDHDTLKDFHSHTYTAPNIFVVAAGKLDPDDVESKVEEKLSGLPGGEPFVR
                     EARPTPPESRFFFKFKETEQYHVSLGSLGIPAKSEDRYAMAALNNVLGGGMSSRLFQE
                     VREKRGLAYAVYSYHQGYSDAGAIKTYVGSTTGNVEEAVRVIVEQLGKIMDEEVEGEE
                     LERTKQQLKSSTLLALESTSARMNRVGRSVITGAELLTPEEISARIEAVTAQDIARLA
                     KQHLRTDRMYLSAVGPEKLDLGRYLN"
     gene            complement(6663..8720)
                     /locus_tag="VN178_09900"
     CDS             complement(6663..8720)
                     /locus_tag="VN178_09900"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWS81323.1"
                     /translation="MRLDIPVGERSIVLETGKLAKQAGGSVTARLGDTVILSTATRSQ
                     APRPGATFLPLSVDIEERMSAAGKIPGGFIKREGRPSERAILTSRLTDRPLRPLFPKN
                     YHYEIQVIGTVLVADQETPYDTVSMVGASATLALSDLPFEGPIGAVRVGRSLDGEFIM
                     NPTYAQIAESDLDLVVAGTKDAITMVEAGANEVPETVMVEALQLAEEAVKAQAEAIEE
                     WAREHGDEKQEIDEAEENPFLADFRERYFDRMKEGIVQTDRRARHDALEELREELTRD
                     REEEEIPQIGAALSTLEKEAFRSLYLEDRKRTDLREFDEIRPTSAEVRVLPRVHGTGL
                     FTRGETQVLSSLALADLGLAQRLDTIEPQTGKRFMHHYNFPPYSTGETGRLGSPRRRE
                     IGHGALAERALVPVIPSEEEFPYALRVISEVLESNGSSSMASVCGSTLALMDGGVPIK
                     APVAGVAMGLVKEGDDYVILSDIQGLEDHMGDMDFKVAGTADGITALQMDMKITGVSA
                     GLLQEALEQAKVGRLQILGILRGVIAEPRAEVSDYAPRVEVIKIPKDKIGMLIGPGGK
                     TINALQEQFGVNISVEDDGTVFVAGEGSISREAASAINGMTKDVEAGEIYTGKVVKTT
                     NFGAFVELTPGRDGLVHISRLAPGRQRVERVEDVVNEGDRIKVRVLEIDKQNRISLEK
                     LEG"
     gene            complement(8756..9022)
                     /gene="rpsO"
                     /locus_tag="VN178_09905"
     CDS             complement(8756..9022)
                     /gene="rpsO"
                     /locus_tag="VN178_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWS81324.1"
                     /translation="MAVVEHKEEIIQEHRTHEGDTGSPEVQVAVLTRRIAHLTEHLRE
                     HKHDYHSRRGLLKMVGRRRRLLKYLQKKDVERYRALIAKLGLRR"
     gene            complement(9125..10021)
                     /locus_tag="VN178_09910"
     CDS             complement(9125..10021)
                     /locus_tag="VN178_09910"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWS81325.1"
                     /translation="MRHVVVALGNFDGVHLGHQEVLRRAVEEGRSRGMRVVAATFHPH
                     PRTVLGSGDPPKLLTPLGLRCEMLLGYGADEVWVIPFDVALSRKSPEQFVKNVLIGEG
                     GAGVVVVGENFRFGHKAAGHFSDLRRLMREAGGTAVGVRVRGGGMGGGISSTRIRALI
                     SGGEVTEAAGLLGRPYVLRGEVVVGDRRGRSIGFPTANVLPDTEAVVPARGVYAGFVR
                     VGEKEYAACTNVGVAPTFGRAESRIEAHLLDFEGDLYGHVVDVGFQERIREERRFSGV
                     DELVGQIRRDVEEARIVAFGPG"
     gene            complement(10009..10887)
                     /gene="truB"
                     /locus_tag="VN178_09915"
     CDS             complement(10009..10887)
                     /gene="truB"
                     /locus_tag="VN178_09915"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWS81326.1"
                     /translation="MTQKVPSGGLLLDKPPGITSARAVSGIKRLLPRKTKIGHTGTLD
                     PLASGLLVLLLGRATRLSRYVTNLDKSYTATARFGAVSDTLDAEGRITDLDSPLPTQD
                     AIREALPAFTGDLLQVPPMASALKRGGVRLYDLHRRGINVEREARPVTVHGFELAALD
                     PTRQTAEFLVSCSSGTYVRTLIDDLATSLGSGAYLTALRRTSVGHLSVENASLPQDLP
                     ETLHNRIIPPTEVISHLPEVAVSGETGRGVVCHGGRIERGWIDGSYRVMCGGELLAVY
                     RDDEEAGRAEVVLCAT"
     gene            complement(10884..11846)
                     /locus_tag="VN178_09920"
     CDS             complement(10884..11846)
                     /locus_tag="VN178_09920"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HWS81327.1"
                     /translation="MKTRTKNNTLAEIAEFLKTLDRVAVTTHVGSDGDAIGASAALVR
                     LMRHLGAEAVFCHAEGAPEYLGWLLPDEPLYELPPDYDLLVADTSRADRTGVPVPQAG
                     ARLNIDHHEDNPLYAEYNLVSPRAAATAEIVADLYAELDVPIDKPTAEALYVGLRTDT
                     GGFNFRNISPRAHELVADLLRAGVVPAEIGERINRRGSIEQLRIVGVSLANAVRYGEA
                     LISTVENSDYERTGATELDSKESIDQLRTVAGVDVVAHLREVPGGTKGSLRSETVDVG
                     EIARRFGGGGHRLAAGYTTPRRPEEAREELLGILEGVLDLGGGR"
     gene            complement(11824..12180)
                     /gene="rbfA"
                     /locus_tag="VN178_09925"
     CDS             complement(11824..12180)
                     /gene="rbfA"
                     /locus_tag="VN178_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWS81328.1"
                     /translation="MSGRTRKVESQLKEIAGEEVASLSDPRIQGLVTVTGVRVSPDLA
                     HATVFYSVLAGEDETAAHEGLQSAAGRIQAAVGTQTRLKRTPRLHFEPDPVVERATRI
                     EAALREVRTDEDAHEE"
     gene            complement(12191..12472)
                     /locus_tag="VN178_09930"
     CDS             complement(12191..12472)
                     /locus_tag="VN178_09930"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWS81329.1"
                     /translation="MFCTVRLELELPFSSSLKDKRQTLRSLKDRLRRKNVSVVESDHQ
                     DLWQRATMEIALAAVSRGAAEEKREELRRTLLTYEELSILDWREEFVKL"
     gene            complement(12482..14635)
                     /gene="infB"
                     /locus_tag="VN178_09935"
     CDS             complement(12482..14635)
                     /gene="infB"
                     /locus_tag="VN178_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWS81330.1"
                     /translation="MSKRVYEIAREQSLESKEVIQRLKEAGVEVKSHSSSVEDNVYER
                     VFGSGAETPNGRTAAQEAEAQEARAQAERSGHGATMAEAPAEEPISEPRQTRPQKRRR
                     RVVIDASARNRGARPAAPPSTTKETERAPVEEEEPESGGVRVEPGATVKDLGDALGVP
                     PTQVIKVLMGLGEMKTLTQTLSTEEIELVAEELGVEVEIGAVEEPEPVEVAPDDDPAD
                     LVERPPVVTVMGHVDHGKTSLLDRIRQSNVQSGEAGGITQHIGAYQVESDGRKVTFID
                     TPGHEAFTEMRARGARVTDVVVLVVAADDGIMPQTAEAIEHARAAGVPIVVALNKIDV
                     PNANQDRVLGELSERELVPEVYGGDTVTVPVSAKTGEGIEELLENILVVAELEDLKAN
                     PKAPASGYVIEGERDPGRGPVATLLLSRGTLEKSDAVLAGTAYGRVRAMLDFMGNRVK
                     EAGPGMPVEILGLSGVPEAGTRFEVVDHERIARDRAQQAEERLRRQELAQGGTRRTLE
                     ELLGEGGTQDLNLVVKADVAGSVEALKEALARLSTDEVRINIVRSGVGALTDSDVMLG
                     SASDGILLGFNVRPTNTAKQVAEREGVEIRTYDVIYKALEEIEAAMRGMLAPDIEERE
                     SGTAEVRQTFRVPNVGVVAGCYVTSGEIFRNNRVRVVRDGTVVYEGNIASLKRFRDDV
                     RSVREGFECGVGVENFNDVKEGDVLEFFETVEVPR"
     gene            complement(14644..15762)
                     /gene="nusA"
                     /locus_tag="VN178_09940"
     CDS             complement(14644..15762)
                     /gene="nusA"
                     /locus_tag="VN178_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWS81331.1"
                     /translation="MNTPLLSALREIETDKGIPFETVKGVLEESLLAAYQGREGAEED
                     ARVVLDEDTGDLRVMKDGEDITPHDFTRIAAQVMRQTFYQRLNEVHNEQLLEEYGERM
                     GDVVTGIVQQAHRRMTLVDLGRVEAILPASEQVPNERYENGQRLKVYLLEIREGGRGP
                     SLTVSRRHEGLLKNLFELEVPEIYDGLVEIRAVAREAGLRSKVAVWSKEAGVDPVGAC
                     VGPRGSRVRAVVSELRNEKIDIIQWDPEPARFIAKALSPARVREVYLDEDEKQAEVIV
                     PDDQLSLAIGREGQNARLAVKLTDWKIDIKPESQATEYEEEEEDWEPDEDSTMHRCRA
                     VLSNGRRCANMALPGSLFCGIPSHQAQAAEFEGMVGRG"
     gene            complement(15759..16211)
                     /locus_tag="VN178_09945"
     CDS             complement(15759..16211)
                     /locus_tag="VN178_09945"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81332.1"
                     /translation="MRETAQLERLREAVEGVLPEGTELVEARFSGGPLLTVLLDREDG
                     PVDHEFCSRVASVVAPAVEAEGYDGPIEVSSPGIERPLTRPEHFRRFVGHEAKVRIGE
                     PLNGRRNFTGVIDRTGEDAFVLRLSEGAEVELPFGSVTRANLKEDIYK"
     gene            16492..16785
                     /locus_tag="VN178_09950"
     CDS             16492..16785
                     /locus_tag="VN178_09950"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWS81333.1"
                     /translation="MPRYVVLVNWTEQGIRNVKETLERTDSGGELAAKHGLKLEQAYW
                     TVGPYDMVTVFEAPNDEALSAHLLEIGSLGNVRTTTLRAYSEEEMSGLLQRLA"
     gene            complement(16832..17905)
                     /gene="ispG"
                     /locus_tag="VN178_09955"
                     /gene_synonym="gcpE"
     CDS             complement(16832..17905)
                     /gene="ispG"
                     /locus_tag="VN178_09955"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWS81334.1"
                     /translation="MTEAALRERPVTSRQIMVGDVPIGGGAQVAVQSMTNTMTYDVEA
                     TVQQVYDLNAAGADLVRVSVNGSKALAGFKEVVWRSPVPLIADIHFDYRMALGAAEGG
                     AACVRINPGNIGSDDRYRQVIEKCQETGTAMRIGVNSGSVEKRYWDLPKAEALVASAL
                     HKVEIAEEMHFFDFKVSLKASHVPEMVEANKKFRGHSDAPLHLGVTEAGTRLPGAIKS
                     AAALGQLLPYGIGDTIRISLAEDPVEEIPVAYQILSALDLRHRGPNVIACPSCARTLG
                     FDVIGMAAKVEEGLKHYEDHFTVAVMGCVVNGPGEARDADYGVAGGKDDGVIFSKGQP
                     LRKVGRDEIYDALFEEIAKDDRK"
     gene            complement(17937..18980)
                     /locus_tag="VN178_09960"
     CDS             complement(17937..18980)
                     /locus_tag="VN178_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HWS81335.1"
                     /translation="MTVIVAILGLIFLIVIHEVGHMLTAKALGVRVPEFGVGFGPALV
                     KKKIGQTVYSFRIILLGGFAKMAGMGDGAEGPDTYYAKALWRRALIILAGPAANLFAA
                     VAILACVYMFQGVVTDVKPVVSAVEQGSMAQEVGIERGDRIVALDGGTVKTWENFERR
                     MSGREVGERVSVTVVRDGSQKTFEGELGADPQDKDRALVGVRPELETTTYGPLESVWL
                     AVQRCAEITVKLGVFVGQLLTGEKDLYDNVTGPVGIASVGSTAVNQGFFLPLLALISL
                     NLAIFNLFPILPLDGGHLFFIAAEKILGRPVSEQTMGRIAALGLVLVLALFLFATYAD
                     LSKIFTGQPFIPD"
     gene            complement(18977..20116)
                     /gene="dxr"
                     /locus_tag="VN178_09965"
     CDS             complement(18977..20116)
                     /gene="dxr"
                     /locus_tag="VN178_09965"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWS81336.1"
                     /translation="MRRRLTILGSTGSIGKQALDVVRRYPDRFEIVGLSAGNDALALA
                     QQAVEFGPEYLALESSESGPLEGLGAEVLAGPGSAERLSRAPSDVVLNGIVGFAGLAA
                     TVGALQTGNRVALANKESLVAGGEWVMDLAASNRIIPVDSEHSAIFQCLEGRRDGEIR
                     ELLLTASGGPFFSYEKAKLMEAGPEDALRHPTWRMGPKITIDSATMMNKGLEVIEAHH
                     LFGVALEDVRVVVHRQSVVHGGAFFEDGSVVLHAALPDMRLPIAFGLLYPERVDVGAE
                     PVPFDGVGWSFEEPRDDVFKCLPLAVEAGRVGGAYPVALNAANEVAVRAFLDGRIRFL
                     RIADAIEEVLEVVPDFGMMQSMEAIVAVDGWARDEAKRRVDGGSR"
     gene            complement(20130..20900)
                     /locus_tag="VN178_09970"
     CDS             complement(20130..20900)
                     /locus_tag="VN178_09970"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWS81337.1"
                     /translation="MLRWRAATALVLAPIVLASIAVGKWAVIAVLLLVIAGAAYELSR
                     ALEPLPFVAALGAGALPLLLSIPYHESGVLAGAVLSLPWALIWLAARPETRTLRAVLA
                     VLLMSLWVGVPLAHLGLFPRSSYGVVLILIAVVGPWISDSGAYFAGRFFGRRLLVPTL
                     SPNKTVEGSVGGLLSTVLIVAPVALLLLDFTIVKALIMGACVSAFSQCGDLFESSLKR
                     ILEVKDLGNFLPGHGGVLDRVDSLLFTAPAVYYISLLV"
     gene            complement(20908..21588)
                     /gene="uppS"
                     /locus_tag="VN178_09975"
     CDS             complement(20908..21588)
                     /gene="uppS"
                     /locus_tag="VN178_09975"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWS81338.1"
                     /translation="MPRHVAIIMDGNGRWAKRRWLPRAAGHRAGVSVLTPLLETAGRA
                     GVQTLTLYAFSTENWTRPEREVKTLMKLFLETAREKVPELNERGARLRFLGRREHLPA
                     SVLEAMEEAEDLTSANDSLDVYIALDYGGRAEIVDAARRMVADGLSPEEIDEMTFAGY
                     LYAPDVPEVDLVIRTSGELRVSNFLLWQIAYAEFYVTDTLWPDFSPKEFMLALESFGS
                     RSRRRGGV"
     gene            complement(21658..>22204)
                     /gene="frr"
                     /locus_tag="VN178_09980"
     CDS             complement(21658..>22204)
                     /gene="frr"
                     /locus_tag="VN178_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWS81339.1"
                     /translation="DLSAAGRRMNGAVESVKSNFATIRTGRANPSILDRIEVELYGSK
                     MSLRSVANIGVPEARLLTVTPFDPNTLKEIERAIRDADIGLNPQNDGKMLRLPIPELT
                     EERRRELIRMARSMAEEGRVSVRNVRRDEMRDVHELRKEGEISQDDEHRAEDELQKLT
                     DGYVKRVEAALADKEAELSEV"
BASE COUNT         3581 a         7552 c         6833 g         4238 t
ORIGIN      
        1 gactccgata gcctgggcgt aggtggggtc gatggcgtgc tcggcgtcga tgaaggcggc
       61 gagacccccc gccttctgcg cctcggcgat gatgtgcagg ccgagggtcg tcttcccgct
      121 cgactcggga ccgaagatct cgactatccg gcccctgggg acgccgccga cgccgagggc
      181 gaggtcgagg gccagggccc cggtagggat ggaggcgacg ctctggcgct cctcgtcgcc
      241 catgcgcatg atcgaaccct taccgaactg ccgctctatc tgggagattg cggcgtctat
      301 agccttgctc ttatccaatg catcctccaa aaagcgcgtg tgcctgcacc ttatgtaatc
      361 ggcatgtact gcgcctgatt atactgtagg ggtctcgggc tcccaagggg ttttggtgct
      421 ttttagctcg cgctcttcag ggtgtagcgc ctggcgacga tctcgcccga gtccccgagc
      481 gcgcccgcgt cctggccgtt gatctccacc cacacggccc ccgcatcccc gctcttgatg
      541 aacagtctgt gctcggcctc gaacgtctcc gagaaccccg gctgggccac ctgcttgtag
      601 gccctattcc cgtcggtccg gatcagtatc cacgagggcc gttctctcac gtcgacgacc
      661 acccgcagcg tgtcgggcgg agcatcctgc ttcgctacca cgggtttctc ctcgctcgtg
      721 cctttgctct caacggcgtc cttgggatcc ttctccgtgc cggccacgtt ctgctgctcc
      781 tggcgaggcg gggtctctcc cggggccggg tcattctcct tcgtggtctg cacgccgcgt
      841 ccgacgaagt acagcgcccc gataacggcc gccagggcga gtacggcgac catcacggtc
      901 gcaacggccg agctggagac cttgcgcttg tccgccgccc tcaggccgct gggtgagatc
      961 aggggcttct cgaagtcgct ctcgggagta gggtcggagt cgctgccgcg ctcgcgctga
     1021 ggttttgtgc tgttcttgag ccgttgcgag agggcttcgc cgtccaggcc caggtaattg
     1081 gcgtaggtct tcagaaagcc cctggcgtag accgcatcgg gtagaaccgc gtagtcctca
     1141 cgctcgaggc ccgtgaggta gcgcttgcgg atcttggtgg cctgctcgac ctcctcgagg
     1201 gagagacccc gctccttacg cttgtgctcg aggaggcggc cgatctcacc cctcgattgg
     1261 tcgccttccg ccggatcttg cgtgtgccgc cagtcttcca tcgcacccaa gagcctacca
     1321 taaatgctac cagccctcgg cccgttcgga cctcccttcg tcctcaccga acaaggtctc
     1381 caggtccatc gtagaagcca ccacggcgcg gctcttggaa ccctcgtagg gcccgacgac
     1441 gcccttgcgc tccagcgcgt cgatgatcct gccggcccgc gagtagccca ccctgaaacg
     1501 ccgctgaacg gcgctcacgg aggcctgttg ggtggagacg acgaagctcg ccgcctcggg
     1561 cagtagatcg tcgtcgggct cgtcgttttc ctccttgacg ctccgcggct ccgtcacctc
     1621 ctccacaaag cgcgcctccg cgcttcccga ggccgcctcg acggtggccg acaccaccct
     1681 gtcgacctcc gcttcggaga tgaaggcccc ctggaccctc gatggtctcg cggcggagac
     1741 gggcttgtag agcatgtccc ccatcccgag cagggcctcg gcgccggggg aatccaggat
     1801 cacccggctg tccacctggc tggagacggc gaaggcgatg cggctcggca cgttggcctt
     1861 gatcataccg gttatcacgt ccacgctcgg tctctgggtg gcgaccacga tgtggatgcc
     1921 caccgcgcgg gctttctggg ccagcctgat gatggcgtcc tcgaccttgg ccccagcctg
     1981 catcatgagg tcggcgagct cgtctatgac cacgacgacg taggggatct ggacctcggc
     2041 cctgtcgttg tatccctcga cggaccttaa cccgttcttc tcgagcacct cgtaacggcg
     2101 ctccatctcg gccacggccc accccagcgc gttggccgcc ttcttgacgt cggtcacgac
     2161 gggcgtgata aggtgcggta cccgcccgaa ctgcgagagc tcgaccctct tggggtctat
     2221 gaggaccatc ttgacctgcc gcgggtcggt cgtgagtaac agtgaggtca aaagcccgtt
     2281 tagcatcacg ctcttgccgc tccccgtggt cccagcgacg agcaagtgcg gcatctcgga
     2341 gaggtcgaag aacacggccc gccccgagat gtctttgccg agtcctactg ggagggtcca
     2401 gtcgttcgcg tcggggtagg cccggaagac atccccgagc gtcaccttcg ccggcctggt
     2461 attgggtact tcgaccccga ccgcgctctt gcccggtatc ggggcgagta tcctgacctc
     2521 ggtcgccgcc agggcatacg ctatgtcctg ttgcaggttc ctgaccttgc cgaccttgac
     2581 cccgctaccg agacgcagct cgtatcgggt gacccgcggg cctaccacgg cgcgcaccac
     2641 gtgggcctcc accccgagat cggagagcgc gagggtgagc ctccggctcg taccttcggc
     2701 gtcgtgctcg ggatcccctt gcccgagact cagcatcgaa aacggcggcg gtgtgtactc
     2761 gccgggtgca agctccctct gggcgctgaa gcccaactcg ggacctcgat cgggcaagac
     2821 gacctcgaac tctcgggtct tcgcaccctc cgacggcccg ctgtcaggct cggcggacac
     2881 cccttcgtcc tcgtcccacg cgtctggttc ggccgtctcg cgaaccttcg cagggtcggt
     2941 acggccacgt ttcgccggtg gctctggtct ccccgacctc ttctcccaca gcccccggcc
     3001 cagacccgcg accttctcac caacaaccct gagcgccccc agcgccgcgg cgaagctaac
     3061 gccggtgagc agcgacaggc cgaccacgta gaggagcgcg atcgtgagcg cggcaccgat
     3121 ggcacccgcg gcccactcca cgcccgcata gattccactt cctagaacgc ccccggcctc
     3181 gggatagtgc cgggcggaga actgatgcct cggcgccaga gtagccgcga agctggccgc
     3241 gccggccagc agcaagagag cggcgccggc gacgaggcgg cccctcaggc ggccgagcaa
     3301 aaggagcagc cccgcgacca ccgcgagcgg ggcgagcgcg agcccaacgc tccccaacaa
     3361 ctgggtgctc acaaaggatc ccgcatcccc gagaaacgac ccgcgtccgc tcgcgaacgc
     3421 ggcgccgaag aacaggccaa cggccaaaag gaagaccccc acggcctccc ggctcaaagc
     3481 cccggtgagg ccctcgcgca cgcgggtcag ggtcgaggta cccttgcgct tcgacttgcc
     3541 cttgccggag gtgcgcttct tcgcagtctt cttcggcgcc cgcttgcccg tagccacacc
     3601 ctaagtctag cccataaacc tctactccac cgtcaaccgc aaggtgtagt acagggttag
     3661 atctaaacct caactattag cggcaggatg atcgggcgct tacccgtacg gtctgagagg
     3721 aagcgggaga gatctttacg gatcgcgttc ttcagttcgc cccagtcggt gacgcggtgg
     3781 ctcgcggtct gctccagggt ctggctgacc acttctatgg actggtcgac gaggccgttg
     3841 gcgctctctt ggctgacgaa gccgcgggag atgacgtcgg ggggtccgag aagctgtccg
     3901 ttctgggcgt ttatgcgggc caccacgata aacatgccgt cttcggagag ttgctggcgc
     3961 tcgcgcatca cgcccgagga gctctcggcg atcgcgcccg cgtcgacgag gaacatccct
     4021 gcggcgacgt tatctgtgcg gtgggcatcg ccgtccacga actccaggca gtcgccgttc
     4081 tccaggataa agatattgtc ctcggggatg ccgaggctca tcgcggtgtt cgcgtggtgg
     4141 cgcaggtgcc tgaactcgcc gtgtacgggg acgaggtagc gcggcttgag gagggagagc
     4201 actatctttt gctcctcctg cgccgcgtgc cccgagacgt gcacgggcat gagggggctg
     4261 tagaacacgt gggcgccgcg cttcatcagg ttgttgatgg tgcgggagac gccgcgttcg
     4321 ttgccaggga tggggtgagc cgcaatcacg accgtgtctt cgggtcccac ctcgatggtg
     4381 cggtgggtcc cgttggcgat cctgctcagg gcagcgaggg gctcgccctg cgagcccgtc
     4441 gtaaggacaa cgatgtcgga gtcggggatc tggcccatgt cccgttggtc gaccatcatc
     4501 tcctgcggca ggtcgaggta gccgagctcg cgggcgatct gcacgttgcg gatcatcgag
     4561 cggcccgtta ccccgacgag cctgtcgtgc tttttggccg cctcgaccac ctggcttacg
     4621 cggtggatgt gcgaggcgaa ggaggccacg atgatgcggc ccttcgcgct ctcgaagacc
     4681 tcgtttaggg tgtccccgac gatcctctcc gagggcacgt agccgggccg ttcggagtta
     4741 gtggagtcgc cgaagtaggc gagcacgcct tccttgccga gctcggcgag gcgccccaaa
     4801 tccatgggtg agccctctat cggggtgagg tcgatcttgt agtcgccgga gaacacgatc
     4861 acgcccgcgt tggtacggat ggctatcgcg acggctccgg gtatggagtg gtttacctct
     4921 atgaactcca atccgaaacc accaaggttc tgccgatcgc cggccttgat ctcgcgcaga
     4981 tctccccgct tgatcccgaa ctcctgcagc ttgctgcgca cgagccctag ggtgagtttt
     5041 gtggcataga cgggcacgtt gaactcacgc aacaggtaag gcagggcgcc cacgtggtcc
     5101 tcgtgaccgt gggtgagcac tatccccttc aactcgtttg cgtgctccct caagtaggtg
     5161 aaatctggga gtacgagatc tatgcccggc atctcctcgt cgggaaacga cataccggcg
     5221 tcgaccagga tcatcccgct atcctggcgc acggccgtca tgttcttgcc gatctcaccc
     5281 aaccccccga ggggaacgac ctgcaaggtc tggttgtcta acagtatttc ctcccttcgg
     5341 tcggaaaagc tgtcagcctt cagctatcag cattgtcgtt cttggctgat tgctgaaagc
     5401 tgacagctaa ttcaggtacc tccccagatc gagcttctcc ggcccgacgg cggagaggta
     5461 catccggtcc gttctcaggt gctgtttcgc gaggcgtgct atgtcctgcg ctgtcacggc
     5521 ctcgatgcgg gcggagatct cctctggcgt caggagctcc gcgcccgtta ttacgctgcg
     5581 gcccacgcga ttcatcctcg ccgaggtgct ctccagggcg agcagcgtcg agctcttgag
     5641 ctgctgcttg gtacgctcca gctcctctcc ctcgacctcc tcatccatga tcttgccgag
     5701 ctgctcgacg atcaccctga ccgcctcctc cacgttgccc gtggtggagc cgacgtaggt
     5761 cttgatcgcc cccgcgtccg agtacccctg gtggtaggag tacaccgcgt aggcgaggcc
     5821 gcgcttctcg cgcacctcct ggaagaggcg gctgctcata ccgccgccca ggacgttgtt
     5881 caaagctgcc atcgcgtaac ggtcttcgct cttcgccggt attcccaggg agcccagcga
     5941 gacgtggtat tgctccgtct ccttgaactt gaagaaaaag cggctctccg gcggcgtcgg
     6001 cctcgcctcg cgcacgaaag gctctcctcc tggcagtccg gagagcttct cttcgacctt
     6061 gctttccacg tcgtccgggt cgagcttgcc cgcggcgacg acgaagatgt tgggggccgt
     6121 gtaggtgtgg ctgtggaagt ccttgagcgt atcgtggtcc accccgcgaa ccgtctcggc
     6181 cgacccgata atcgggcgtc cgagggggtc tgcgtggaag atcaaagacg acaggtactc
     6241 gtcggcgagc tggtccggcc tgtcctcgta catcttgatc tcttcgacta tgacctcgcg
     6301 ttccctctcg aggtcgacga gcgtgggcct caggaccatg tcgctcatga tgtcgagggc
     6361 gcgccccagg tgttcgggca ggaagcgggc gtagagcacg gtgtactcct cgccggtcgc
     6421 cgcgttctcc tgggccccga tgccctcgaa ggcctgggcg atgccaaggg cgtccatttc
     6481 gggggtcccc ttgaagagca tgtgctccat caggtggctg atcccggcga cctcgtccgt
     6541 ctcgtcgcgc gagccggccc ggatccagag ccccaatgcg acgctcgtcg cctcggccag
     6601 gggctcggag aagaccctga ggttcccggg aagatccttg cgccttatgt tcggatcggc
     6661 cactagccct ccagtttctc gaggctgatg cggttctgct tgtctatctc caagacacgc
     6721 accttgatgc ggtcgccctc gtttacgacg tcctcgaccc tctcgacccg ctgccttcct
     6781 ggcgcgagac gtgagatgtg cacgagcccg tcgcggcccg gcgtgagctc gacgaaggcc
     6841 ccgaagttgg tggtcttcac gaccttgccg gtgtagatct caccggcctc gacgtccttc
     6901 gtcatcccgt ttatcgcgga ggccgcctcc ctggatatgc tcccttcacc ggccacgaag
     6961 acggttccgt cgtcctcgac ggagatgttc acgccgaact gctcctgaag cgcgttgatg
     7021 gtcttcccgc ctggccctat cagcatcccg atcttatcct tcgggatctt tatgacctcg
     7081 accctcgggg cgtagtcgga gacctcggcc ctcggctcgg ctatgactcc gcgcaggatc
     7141 ccgagtatct gcaacctccc gaccttcgcc tgctccagcg cttcctgcaa gagcccggcc
     7201 gagacgccgg tgatcttcat gtccatctgc agcgccgtga tgccgtcggc cgtgccagcg
     7261 accttgaagt ccatgtcgcc catgtggtcc tccaagccct ggatgtcact gaggatgacg
     7321 tagtcgtcgc cctccttgac cagccccata gccacaccgg caacgggcgc cttaatcggc
     7381 acgccgccgt ccatcagcgc gagcgtcgaa ccgcacacgc tcgccatcga tgaggaaccg
     7441 ttcgactcga gaacctcgga tattacgcgt agcgcataag ggaactcttc ctccgaagga
     7501 atgaccggca ccagcgcccg ttcggcgaga gcgccgtgcc cgatctcacg ccgccgcggg
     7561 ctgcccaacc ggcccgtctc gccggtggag tagggcggga agttgtagtg gtgcatgaag
     7621 cgcttacccg tctgcggctc gatggtgtcg agtcgttgcg cgagacccag gtccgccagg
     7681 gccagcgagc tcaagacctg cgtctcgccc ctcgtaaaga ggcccgtgcc gtgtacgcgt
     7741 ggcagcaccc gcacctcggc cgaggtcggc ctgatctcgt caaactcgcg caggtccgta
     7801 cgcttgcgat cctcgaggta gagagaccgg aacgcctcct tctcgagggt cgagagagcc
     7861 gcgccgatct ggggtatctc ctcttcctcg cggtcccggg tcaactcttc gcgcaactcc
     7921 tcgagcgcgt cgtggcgggc cctgcggtcg gtctggacga tgccttcttt catccggtcg
     7981 aagtaccgct cccggaagtc ggcgaggaac gggttctcct cggcttcgtc tatctcttgc
     8041 ttctcgtccc cgtgctctct ggcccactct tctatcgcct cggcctgggc cttgacggcc
     8101 tcctcggcga gctgtagggc ctctaccatg acggtctcgg gcacctcatt cgcccctgcc
     8161 tcgaccatgg ttatggcatc tttcgtcccc gcgaccacaa ggtccaagtc gctctccgcg
     8221 atctgggcgt acgtggggtt cataatgaac tcgccatcca gcgaacgccc gacgcgcacg
     8281 gccccgatcg ggccctcgaa gggcagatcg gagagggcga gcgtcgcgga ggccccgacc
     8341 atgctaacgg tgtcgtaagg ggtctcctgg tcggcgacca gaacggtgcc tataacctgt
     8401 atctcgtagt gatagttctt ggggaagagc ggcctgaggg gcctgtccgt gaggcgcgag
     8461 gtcaggatcg cccgctccga aggcctaccc tcgcgcttga taaagccgcc ggggatctta
     8521 cccgccgcgg acatgcgctc ctcgatgtcc acgctgagag gcaagaacgt cgcgccaggc
     8581 cggggagcct gcgagcgtgt cgccgtcgaa agtatcacgg tgtcgccgag tctcgccgtc
     8641 accgagccac cggcttgctt ggcaagcttc ccggtttcga gcacgatcga gcgctcaccg
     8701 actggtatgt ctagtcgcat gttgttttct cctctggctt ttctttttac ccggactacc
     8761 ggcgtagacc gagcttggcg atgagcgcgc ggtagcgctc gacgtccttt ttctgcagat
     8821 actttagtaa cctgcggcgc ctgccgacca tcttcagcag tccacgccgc gaatggtagt
     8881 cgtgcttgtg ctcgcgcaag tgctcggtaa ggtgcgctat gcgccgcgtg agaaccgcaa
     8941 cctgcacctc gggagacccc gtgtccccct cgtgcgtccg gtgctcttgg atgatctcct
     9001 ctttgtgctc aactacagcc aacttcttct ttctcctctt tgttcacttc tctctctgtt
     9061 taacccgttc gactctttat gctaccacat gcatcaatta gtatcgtgtg ccaaagacca
     9121 cagatcaacc cggtccgaaa gctacgatcc tggcctcctc cacgtcgcgc cgaatctgcc
     9181 ctacaagctc atctacgccc gagaatcgcc gctcctccct tatgcgctct tggaagccga
     9241 cgtccacaac gtgcccgtag agatccccct cgaaatcgag gagatgggcc tcgatgcggc
     9301 tctcggctcg cccgaaggtg ggagcgaccc cgacgttagt acaggcagca tactccttct
     9361 cgccgacgcg tacgaagccg gcgtagactc cgcgggccgg aaccaccgct tctgtatcgg
     9421 gtagcacgtt ggccgtgggg aaacctatcg acctgccgcg cctgtctcct acgaccacct
     9481 cgcccctgag cacgtagggg cgtccaagga ggccggcagc ctcggttact tctccaccgg
     9541 agatcagagc ccgtatgcgg gtggagctga tcccaccccc catgcccccg ccgcgtaccc
     9601 gaacgccgac ggccgttcca cccgcctccc gcatgagccg gcggaggtca ctgaagtggc
     9661 ccgcggcttt gtgcccgaaa cgaaagttct ccccgaccac gaccaccccg gccccaccct
     9721 cgccgatcag cacgttcttt acgaactgct ctggactctt cctggacagc gcaacgtcga
     9781 acggtatcac ccatacctca tcagccccgt atcccagaag catctcacac cgtaacccca
     9841 atggggtaag caacttcggc ggatcaccac tccccaatac ggtccgtgga tggggatgga
     9901 atgtggccgc aacgaccctc atgcccctgc tacgcccctc ctcgacggcc cgtctcaaaa
     9961 cctcctggtg gccgaggtgc acgccgtcga agttccccag ggctaccact acgtggcgca
    10021 aagcaccacc tcggcccgcc ctgcctcctc gtcatccctg tataccgcca acagctctcc
    10081 cccgcacatc acccggtaac tgccgtcgat ccaacccctc tctatgcgcc cgccgtggca
    10141 taccacaccc cggcccgtct cccccgaaac agccacctca ggcaggtgcg agatcacctc
    10201 cgttggaggt attatgcggt tatgtagggt ctctgggagg tcttggggga ggctggcgtt
    10261 ttctacgctg aggtgtccga cgctcgtgcg gcgcagggcg gtgaggtagg cgcccgagcc
    10321 gagggaagtg gcgaggtcgt ctatgagggt tctaacgtag gtgccgctgc tgcaggagac
    10381 caggaactcc gccgtctgcc gtgtagggtc gagggcggcg agttcgaagc catgtaccgt
    10441 gacgggacgt gcttcgcgtt ccacgttgat gccgcggcgg tgcaggtcgt agagccttac
    10501 gcctcccctt ttgagggccg aggccatcgg cggaacctgg aggaggtcgc ccgtaaaggc
    10561 ggggagcgcc tcgcggatgg cgtcttgtgt ggggagtggg gagtcaaggt cggtgatacg
    10621 gccttccgcg tcgagcgtat ccgagacggc accgaagcgg gcggtcgccg tgtaggactt
    10681 gtcgaggttg gtaacgtaac gggagagacg ggtcgcgcgt ccgaggagga gcacgaggag
    10741 gcccgaggcc agggggtcga gggttccggt gtgcccgatc tttgtcttcc tgggtagaag
    10801 gcgcttgatc ccggagaccg ccctggcact cgttatgcca ggcggcttgt ccagcagaag
    10861 gccgcctgag gggactttct gcgttatcgc ccgcccccga gatcgaggac cccttcgagt
    10921 attcccagga gctcttccct ggcctcttcg ggtcggcgcg gcgtggtgta accggcggcg
    10981 agcctgtggc cgcccccccc aaagcggcgt gctatctctc cgacgtcgac cgtctcggag
    11041 cggagagagc ctttcgtgcc cccaggtacc tcgcgcaggt gggcaacgac gtcgacgccg
    11101 gcgacggtgc gcagttggtc gatggattct ttcgaatcga gctccgtcgc gccggtccgc
    11161 tcgtagtccg agttctcgac cgtggagatc agggcctccc cgtaacgaac ggcgttcgcg
    11221 agcgagacgc cgacgatcct gagctgttcg atgctgcccc tgcggttgat acgctctcct
    11281 atctcggcgg gcacgacgcc ggcccgcagg aggtcggcta cgagctcgtg ggcgcggggg
    11341 gagatgttac ggaagttgaa gccgcccgtg tcggtcctga ggcccacgta gagggcctcg
    11401 gcagtaggct tgtcgatggg gacgtccagc tcggcgtaga ggtcggccac gatctccgcc
    11461 gtcgcggcgg ccctcgggct caccaggttg tactcggcgt agagcgggtt gtcctcgtgg
    11521 tggtcgatat tcaggcgggc gcccgcttgg ggtacgggta ctcccgttcg gtctgcgcgg
    11581 gatgtgtcgg cgacgagcag gtcgtaatcc ggggggagct cgtacagcgg ctcgtcgggc
    11641 aagagccagc ccaggtattc gggcgcccct tccgcgtggc agaacacggc ctcggcccca
    11701 agatggcgca tcagacgcac gagggcggcc gaagcgccga tggcatcgcc gtccgaaccc
    11761 acgtgcgtcg ttacggcgac gcggtccagg gtcttcaaga actccgctat ctcggccagc
    11821 gtattattct tcgtgcgcgt cttcatcagt ccttacctcc ctaagtgcgg cttcgatcct
    11881 ggtggcacgt tcgacgacgg ggtcgggctc gaagtgcagc cttggcgtgc gcttgagacg
    11941 ggtctgggtc cctacggcgg cctggatgcg gccggcggcg ctctgcagac cctcgtgtgc
    12001 ggccgtctcg tcttcgccgg cgagcacgct gtagaacacc gtcgcgtggg cgaggtcggg
    12061 gctcaccctg acgccggtga ccgtgacgag gccctggatg cgcgggtccg agagcgaggc
    12121 cacctcctcg cccgctatct ctttgagctg cgactccacc ttgcgcgtgc gaccgctcat
    12181 gacttcgccc tcatagcttc acgaactcct cgcgccagtc gaggatggag agctcctcgt
    12241 aggtgaggag cgtcctgcgg agctcttccc gcttctcctc ggccgccccg cgcgagaccg
    12301 cggcgagcgc gatctccatc gttgcgcgct gccagaggtc ctggtgatcc gactccacca
    12361 cggagacgtt cttgcggcgc agccggtcct tgagggagcg aagcgtctgg cgcttatcct
    12421 tcaggctcga agagaaaggc agctccaact ccagccttac cgtgcagaac aaaggccgac
    12481 tttacctcgg gacctctacg gtctcgaaga actccaggac gtctccctct ttgacgtcgt
    12541 tgaagttctc gacgccgaca ccgcactcga aaccttcgcg caccgaacgg acgtcgtccc
    12601 tgaagcgctt cagggaggct atgttgccct cgtagaccac ggtgccgtcg cgcaccaccc
    12661 gaacgcggtt gttgcggaag atctccccgc tcgtgacgta gcagccggcg acgaccccga
    12721 cgttggggac cctgaaagtc tggcgcacct cggcggtacc gctctcgcgc tcctctatat
    12781 ccggggcgag cataccgcgc atggcggcct cgatctcttc gagggccttg tagatgacgt
    12841 cgtaggtccg gatctcgacg ccttcgcgct cggcgacctg tttggccgtg ttcgtgggcc
    12901 tgacgttgaa accgagcagg atgccgtcgc ttgccgagcc gagcatgacg tcggaatcgg
    12961 taagggcccc gacgccgctg cgcacgatgt tgatgcgcac ctcgtccgtg gagagcctgg
    13021 cgagcgcctc cttgagcgcc tccacggaac cggcgacatc cgccttgacg acgaggttga
    13081 ggtcctgggt gccgccctcg ccgagcagct cttccagggt acggcgcgta ccgccctgcg
    13141 cgagctcctg ccggcgcagg cgctcttcgg cctgctgggc acggtcgcgc gcgatccgct
    13201 cgtggtcgac gacctcgaag cgggtacccg cttcggggac gcccgagagc ccgaggatct
    13261 cgaccggcat ccccggtcct gcctccttga cacgattgcc cataaagtcg agcatcgccc
    13321 ttaccctgcc gtaggccgtt ccggcgagca cggcatcgct cttctctagc gtcccgcgac
    13381 tcaagagcag cgtggcgacg ggaccgcgtc cggggtcgcg ctccccttct ataacgtacc
    13441 cgctcgcggg ggccttgggg ttcgccttga gatcttcgag ctcggccacg accaggatgt
    13501 tctccagaag ctcctcgatt ccctcgccgg tcttggccga cacgggcacc gtgacggtgt
    13561 ccccgccgta gacctcgggc acgagctccc tctcggagag ctcgccgagg accctgtcct
    13621 ggttcgcgtt cggcacgtcg atcttgttta gggccaccac tatgggcacg ccggcggccc
    13681 tcgcgtgctc gatggcctcg gccgtctggg gcatgatgcc gtcgtcggcg gccacgacga
    13741 gcacgaccac gtcggtcacc cgcgcaccgc gggcccgcat ctccgtgaac gcctcgtggc
    13801 cgggggtgtc tataaaggtg accttcctgc cgtccgactc cacctggtag gccccgatgt
    13861 gctgcgtgat accgccggcc tccccggact ggacgttgga ttgccggatc ctgtccaaaa
    13921 ggctcgtctt gccgtggtcc acgtggccca taaccgttac caccggcggc ctctcgacca
    13981 ggtcggcggg gtcgtcgtcg ggcgcgacct cgaccggctc gggctcctcg accgcgccga
    14041 tctcgacctc caccccgagc tcctcggcca ccagctctat ctcctcggtc gacagagtct
    14101 gggtgagcgt cttcatctcc ccgaggccca tcaagacctt gatgacctgg gtcgggggga
    14161 cacccagggc gtcgcccaga tccttgaccg tcgcccccgg ctcgacccgc accccgccgc
    14221 tctcgggttc ttcctcctct acgggagccc gctcggtctc cttggtggta gacggcggcg
    14281 cggccggacg cgccccacgg ttcctcgcgc tcgcgtctat aactaccctg cggcgcctct
    14341 tctggggccg ggtctgccgc ggctccgaga tgggctcttc ggccggcgcc tccgccatgg
    14401 tggcgccgtg tccggagcgt tcggcttgcg cgcgggcttc ctgcgcctcg gcctcctggg
    14461 ccgccgtgcg gccgttgggc gtttcggcgc cggacccgaa gaccctctcg tagacgttgt
    14521 cctcgacgga ggaggagtgg ctctttacct ccaccccggc ctccttgagg cgctgtatga
    14581 cttctttgct ctccagcgac tgctcgcggg caatctcgta tacgcgtttg ctcatcagat
    14641 cccctaccct ctacctacca tgccttcgaa ctccgcggcc tgcgcctggt gggaagggat
    14701 accgcagaac agcgagcctg gcaacgccat gttggcgcac ctgcgcccgt tggagaggac
    14761 cgccctgcag cggtgcatgg tcgagtcctc gtccggctcc cagtcctctt cctcctcctc
    14821 gtattcggtc gcctggctct cgggctttat gtctatcttc cagtccgtaa gcttgacggc
    14881 gaggcgggcg ttctggccct cgcggccgat ggccagcgag agctggtcgt cgggtacgat
    14941 cacctcggcc tgcttctcgt cctcgtccag gtagacctcg cgtacccgcg ccgggctcaa
    15001 cgccttggca ataaagcgcg ccggctcggg gtcccactgg atgatgtcga tcttctcgtt
    15061 cctgagctcg gagacgacgg cccgcacgcg gctcccgcgc ggccccacgc aggccccgac
    15121 gggatccaca ccggcctcct tggaccacac ggccaccttc gagcgcagcc cggcttcgcg
    15181 ggccacggcc cttatctcga cgaggccgtc gtagatctcg ggcacttcga gctcgaagag
    15241 gttcttgagc aggccctcgt ggcgccggct cacggtcagg ctggggccgc gccctccctc
    15301 cctgatctcc agcaggtaaa ccttcagccg ctgcccgttc tcgtaacgct cgttcggcac
    15361 ctgctcgctg gcaggcagta tagcctcgac cctacccagg tcgaccagcg tcatccgccg
    15421 gtgggcctgc tgcacgatac ctgttaccac atcgcccata cgctcgccgt actcctcaag
    15481 gagctgctcg ttgtgtacct cgttcaggcg ctggtagaaa gtctggcgca ttacctgggc
    15541 ggctatgcgc gtaaagtcgt gcggggttat gtcctcgccg tccttcatga ccctgaggtc
    15601 gccggtatcc tcgtcgagga cgaccctcgc gtcctcctcg gccccctcac ggccctggta
    15661 agccgcaagc aacgactcct cgagcacacc cttaaccgtc tcgaagggga tgcctttgtc
    15721 cgtctctatc tcacgcagcg cggatagcaa tggcgtattc acttgtatat gtcctccttg
    15781 aggttcgcgc gggttacgga accgaacggc agctccacct ccgctccctc ggagagtctc
    15841 agtacgaagg cgtcttcacc cgtcctgtct atgacgcccg tgaagttccg gcgcccgttc
    15901 aggggttctc cgatcctgac cttcgcctcg tgcccgacga aacgccggaa gtgctcgggc
    15961 ctcgtcagcg gacgctcgat gcccggagac gagacttcga taggaccatc gtagccttcg
    16021 gcctcgaccg ccggagcaac gacggaggcg acccgcgagc agaactcgtg gtccacggga
    16081 ccgtcctccc ggtccaggag cacggtgagc aaaggccccc ccgagaacct ggcctcgacc
    16141 agctcggttc cctcgggaag cacgccttcg accgcctctc tcagcctctc taattgcgcc
    16201 gtctctctca ccttctccga acgataaaaa aagcaacggg cccgaagacc ccttgctcac
    16261 ttacagcatc cccgattata tctcagagta tctaggttac aaggcgcaag aaccgtctta
    16321 aatcaccatc caacttctat ccggcacgat gagccaggta gaggggccgg agcacggtgc
    16381 cgacccccta agtctttcga cggatgcgcg ggacccggcc cgctggcatc atggtcgaag
    16441 ttggaggcgg caagactcgc ctggttcttc gggcgaaaga gaggaggacg tatgccccgc
    16501 tatgtggtcc tggtgaactg gaccgagcaa ggtatcagga acgtcaagga gaccctcgag
    16561 cgtaccgaca gcggcggcga gctcgccgcg aagcacgggt tgaagttgga gcaagcctac
    16621 tggaccgtcg gtccctacga catggtcacc gtcttcgagg cgccgaacga cgaagccctg
    16681 agcgcccacc ttctggagat cggctctctt ggcaacgtgc gaaccaccac gctccgggcg
    16741 tacagcgaag aagagatgtc agggttactc cagaggctag cctgagcgag ccctgcaacc
    16801 cggatgcgtt cggctttcgg gaaggctctt actacttcct gtcgtccttc gctatctctt
    16861 cgaagagcgc gtcgtagatc tcgtcgcggc ccaccttccg cagaggctgg cccttggaga
    16921 agatcacacc gtcgtccttg cccccggcca cgccgtagtc ggcgtcgcgg gcctcaccgg
    16981 gaccgttgac cacgcagccc atgacggcga cggtgaagtg atcctcgtag tgcttgagcc
    17041 cctcctcgac cttcgcggcc atcccgataa cgtcgaaacc gagcgtgcgc gcgcaactcg
    17101 gacacgcgat gacgtttggc ccacgatgcc ttaggtcgag ggccgagaga atctggtagg
    17161 caacgggtat ctcctcgacc gggtcctccg ccagcgagat gcggatggta tccccgatgc
    17221 cgtagggcag gagttggccc agagccgcag cgctctttat ggcaccgggt agcctggtac
    17281 cggcctcggt aacaccgagg tgcagcggcg catcggagtg cccgcggaac ttcttgttgg
    17341 cctccaccat ctcggggaca tggctcgctt tgagcgagac cttgaaatcg aagaagtgca
    17401 tctcttcggc gatctccacc ttatgcaagg cgctcgccac gagcgcctcg gccttgggga
    17461 ggtcccagta ccgcttctct acggaccccg agttgacccc gatgcgcatc gccgtgcccg
    17521 tctcttggca cttctcgatc acctgccggt agcggtcgtc gctccctatg ttgcccgggt
    17581 tgatccggac acaggcggcg ccaccttctg ccgcccccag cgccatcctg taatcgaagt
    17641 ggatgtcggc gataaggggt acgggcgagc gccacacgac ctccttgaaa ccggcgaggg
    17701 ccttcgagcc gttgacggag acgcgtacca aatcggcgcc cgcggcgttg aggtcgtata
    17761 cttgctgcac cgtcgcctcg acgtcgtatg tcatggtgtt cgtcatcgac tgcacggcca
    17821 cctgggcgcc gccgccgatg ggcacgtcgc ctaccattat ctgccggctc gtaaccgggc
    17881 gttcgcgcag tgctgcctct gtcatctcac tcccacacgt tctttacgca cctatcttaa
    17941 tccgggatga acggctgtcc agtgaagatt ttactcaggt cggcgtaagt cgcgaaaagg
    18001 aacagggcca gaaccaagac gaggccgagc gcggcgatcc tgcccatcgt ctgctcgctt
    18061 actggcctgc ccaggatctt ctccgccgct ataaagaaca ggtgcccgcc gtcgaggggt
    18121 aggatcggga acaggttgaa tatagccaga ttgaggctga tgagtgccag aagcggcagg
    18181 aagaacccct ggttgacggc cgtgctcccc accgaggcga tgcccacggg accggtcacg
    18241 ttgtcgtaca agtccttctc gccggttaag agctgcccga cgaagacccc gagcttgacc
    18301 gtgatctccg cgcacctctg gaccgccagc cagacggact ctagcggccc gtaagtggtc
    18361 gtctccaact cgggacgcac gccaacgagc gcccgatctt tatcctgggg atccgccccc
    18421 aactctccct cgaaggtctt ctgtgaaccg tcgcgcacca cggtcacgct caccctctcg
    18481 ccaacctccc ggcccgacat ccggcgttcg aaattctccc aggtctttac tgtcccacca
    18541 tccagggcca cgatcctgtc tccgcgttcg atcccgacct cctgcgccat cgaaccctgc
    18601 tcgacggcgc ttacgaccgg cttcacgtcg gtgacgacgc cctggaacat gtagacgcac
    18661 gccagtatgg cgacggccgc gaagaggttg gcggcgggtc cagcaagaat gatgagggcc
    18721 ctcctccaca acgctttggc gtagtaggtg tcgggcccct ccgccccgtc ccccatcccc
    18781 gccatcttgg caaaaccgcc tagcagtatt atgcggaaag agtagacggt ctggccgatc
    18841 ttcttcttga cgagagccgg cccgaagccg acgccgaact cggggaccct caccccgagg
    18901 gccttggccg tgagcatgtg cccgacctcg tggatgacta tgaggaagat cagacccagt
    18961 atcgcgacta ttacggtcat cgcgaccctc cgtccacgcg tcttttggcc tcatcccgcg
    19021 cccatccgtc cacggctact atggcttcca tgctctgcat catcccgaag tccggcacca
    19081 cctcgaggac ctcctcgatc gcatctgcta tacgtaggaa tcttatcctg ccgtcaagga
    19141 aggcccggac ggcgacctcg ttggccgcgt tgagcgcgac aggataggcg ccgcctacgc
    19201 gaccggcctc cacggcgagc ggaagacatt tgaatacgtc atcgcgcggc tcctcgaagc
    19261 tccacccgac cccgtcgaag ggcacgggct cggccccgac gtcgaccctc tcggggtaga
    19321 ggagcccgaa ggctatgggc agcctcatgt cgggcaacgc ggcgtgcaac acgaccgagc
    19381 catcctcgaa gaaagcaccc ccatggacca cggactggcg gtgcacgacg acccgcacgt
    19441 cttccaatgc aaccccgaag aggtggtgcg cctctatcac ctccagcccc ttgttcatca
    19501 tggtggccga gtctatggtg atcttcggcc ccatccgcca cgtggggtgc ctgagcgcat
    19561 cctccggccc ggcctccatg agcttcgcct tctcgtagga gaagaagggg ccgcccgacg
    19621 ccgtcaatag tagctccctg atctccccgt cccgcctccc ctccagacac tggaagatcg
    19681 ccgagtgctc agagtctacg ggtattatgc ggttacttgc cgcgaggtcc atgacccact
    19741 cgccccctgc gacgagggat tctttgttgg ctagtgccac gcggttgcct gtttgcaggg
    19801 cgccgacggt ggcggcgagg ccggcgaagc cgactatgcc gttcagtacg acgtctgagg
    19861 gcgcgcggga gagtcgctcg gccgagccgg gaccggcgag gacttcggcg ccgagacctt
    19921 ccaggggacc tgattcgctc gattccagag ccagatactc gggcccgaac tcgaccgcct
    19981 gctgggcgag cgcaagggca tcgttgcccg ccgagaggcc gacgatctcg aatcggtcag
    20041 gatatctgcg gacgacgtcg agggcttgct tgccgatgct gcccgtcgag ccgaggatcg
    20101 tgaggcggcg tctcatgttc ggtccgatct catacgagta gggagatata gtataccgcc
    20161 ggggcggtga agagcaggct gtccacccgg tcgaggacgc ctccgtgacc ggggaggaag
    20221 ttccccaggt ccttcacttc gaggatacgc tttagggaag attcgaagag gtccccgcac
    20281 tggctgaacg cggagacgca ggcgcccata atgagtgcct tgacgatcgt aaagtcgagg
    20341 agcaggaggg cgacgggggc gacgatcaaa accgtcgaca ggaggccgcc gacgctacct
    20401 tccacggtct tgttcgggct cagggtcggt accagcaatc tacggccgaa aaagcgcccg
    20461 gcgaagtacg cgccagagtc ggagatccag ggtcccacta cggctatgag gatgagaacg
    20521 acgccgtagc tggagcgggg aaagagtccc aggtgcgcca gagggacgcc gacccagagc
    20581 gacatcagga gtacggcgag tacggcgcgc agcgtcctcg tctcgggcct ggcggcgagc
    20641 cagatcaggg cccagggcag gctcaggacg gcgcccgcca gtacaccact ctcgtggtaa
    20701 gggatggaga gtagcagggg cagcgcgccg gcgccgaggg ccgcaacgaa aggaagcggc
    20761 tcgagcgccc gggagagctc gtaggcggcc cccgctatga ccaggagtag tacggcgatc
    20821 acggcccatt tgccgacggc tatgctggcc agtactatcg gcgccagcac cagggcggtc
    20881 gctgccctcc accggagcac ggggacccta gacgccgccc cggcgccggg accgggaccc
    20941 gaaagactcg agggccagca tgaactcctt gggggagaag tcgggccaca gcgtatccgt
    21001 cacgtagaac tcggcgtagg ctatctgcca gagcaagaag ttggagacgc gcagctcacc
    21061 gctggtccgg atcaccaaat ctacctcggg tacgtccggg gcgtagaggt aacctgcgaa
    21121 ggtcatctcg tcgatctcct caggagagag accgtccgcg accatccgcc gggcggcatc
    21181 cacgatctcg gcgcgtccac cgtagtcgag ggcgatgtag acgtcgaggc tgtcgttggc
    21241 cgaggtaagg tcctcggctt cctccatcgc ttccagcacc gaggcaggca ggtgctccct
    21301 cctgcccagg aaacggagcc tggcaccacg ctcgttcagc tcgggcacct tctcgcgcgc
    21361 cgtctccagg aagagcttca tgagcgtctt tacctctcgc tccgggcgcg tccagttctc
    21421 ggtcgagaag gcatagaggg tcagcgtctg taccccggcg cgcccggcgg tctccagcaa
    21481 cggcgtgagc accgagacac ccgccctgtg gccggccgcc cgtggaagcc agcggcgctt
    21541 tgcccagcgc ccgttgccgt ccatgatgat ggcgacatgg cgcgggatcc gctcggggtc
    21601 gaggacacgc tgctcggggg aatctgtggg agatcgtttc tcggcgggtt ccagcagtta
    21661 gacctccgag agctcggctt ccttgtcggc aagggcagcc tcgaccctct tcacgtatcc
    21721 gtcggtgagc ttctgtagtt cgtcctcggc cctgtgctcg tcgtcctggg agatctcgcc
    21781 ttctttgcgc aactcgtgca cgtccctcat ctcgtcgcgg cgcacgtttc tgaccgagac
    21841 ccttccctcc tcggccatgc tcctcgccat cctgatcagt tcccggcggc gctcctccgt
    21901 cagttcgggg ataggaagcc tcagcatctt gccgtcgttc tgcgggttga ggccgatatc
    21961 cgcatccctt atggcacgct cgatctcttt gagggtattc ggatcgaagg gggttaccgt
    22021 caggagccgt gcctccggga cgccgatgtt ggcgacgctc ctcaaggaca tcttcgaccc
    22081 gtagagttcg acctcgatcc tgtccaggat cgaggggttc gccctgcccg tgcgtatcgt
    22141 cgcgaaattg ctcttgaccg actcgacggc cccgttcatc ctgcgtccgg ccgccgacag
    22201 atct
//
LOCUS       DATMVF010000200         5514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_621013, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000200 DATMVF010000000
VERSION     DATMVF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621013"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VN178_09985"
     CDS             complement(<1..1341)
                     /locus_tag="VN178_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS81340.1"
                     /translation="MSIRWRLTLFIALVIGVILLVLGLSLYFLIRGMLLKDVEVTAKS
                     RGEAVAHTYESGDQLSTAPNDDDQILFDDSVAVIIRGKNGKVIDKFNLPAGGSTADSD
                     VWRKALKTGQDASDKRMRLGDNDYYVYAVAMDPSARPARVVEFGKSYNETEEILDIFA
                     TVLAFVIGAAFLLSIGGAYQLAGAALRPVDSVPNAASKMGEGDLSKRLPVANPKDEIG
                     RLVITINGLLSRLEAAFVRMQESLERQRRFAADASHELRTPLTSIHGYAQMLKEWALK
                     DPQLGPESVEAIKRESERMKGLIESLLALARGDEGAELKIGRHDLAAVAEEPARATRD
                     ANNGKVSIECVLPEGEVEATFDRDRAFQVAAILLDNAVRYTPEGGKVTVGVREDGDRV
                     ALEVSDTGVGIPEDQLPLIFERFHRADPSRSDGGAGLGLSIARQIAEAHGGQIRA"
     gene            complement(1346..1996)
                     /locus_tag="VN178_09990"
     CDS             complement(1346..1996)
                     /locus_tag="VN178_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS81341.1"
                     /translation="MEDDRAIAGFVEPELEHLGFLVRCAYDGPTGLKEAERFGPALIV
                     LDIMLPGLDGVGVLRRLREGGSRVPVIMLTARDTTLDKVHSLDRGADDYLTKPFDIEE
                     LIARIRALLRRVEGDEILRVADLEINTSTREVRREEREIELTTREYELLAFMARNPRR
                     VLTRDLLLSHVWDGESGLTPNVVDVYVGYLRRKVDAPGEEKLIRTVRGAGYALKGE"
     gene            2329..3480
                     /gene="sucC"
                     /locus_tag="VN178_09995"
     CDS             2329..3480
                     /gene="sucC"
                     /locus_tag="VN178_09995"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWS81342.1"
                     /translation="MDLYEYQGKELLREYGLETLPGIVAGTPEEAERAAETLGGTVAI
                     KAQVLTGGRGKAGGIKVVKSPREAEEAAEQILGMDIRGHTVGRVYVEHGEEIEREFYL
                     SITVDRTKKRPLILFSTEGGVEIEEVAETNPGAIVRRHVDPLLGLLPYQVREITFAAG
                     LEGDVAKGVGMVLSHLYKAFEGLDASLVELNPLVVTREGRVLTLDAKVTVDDSSLYRH
                     EDLAGLHDVEAADPQEERAQEVGLQYVKLDGDIGILGNGAGLVMSTLDVVAQAGGEPA
                     NFCDVGGGADAEQISTALDIVTSNEQVRSVLFNIFGGITRGDEVARGLLSAIERTEIT
                     LPIVVRLDGTNAEEGRRILAENTPENVVTEETMLEAASRAVELARDGGR"
     gene            3485..4360
                     /gene="sucD"
                     /locus_tag="VN178_10000"
     CDS             3485..4360
                     /gene="sucD"
                     /locus_tag="VN178_10000"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWS81343.1"
                     /translation="MAILVDDGTRLLVQGITGREGAFHTGRMLQSGTRVVAGVTPGKG
                     GQDYEGVPIFDTVGEAVGQKGANASVIFVPPPFAADAIFESLDAGLSTICCITEGIPV
                     HDMMRVANYLKNKDATLVGPNCPGVLSPEKSNVSIMPKDIFMPGNVGVVSRSGTLTYQ
                     IVNELTQREIGQSTAVGIGGDPIIGTDFIEILSKFEADPETECAVMIGEIGGNAEQLA
                     AEYIRGEMSIPVVAYIAGFTAPEGKTMGHAGAIVSGGESTAEAKSEALREAGIRAATN
                     PSEVAELVAEALGRS"
     gene            4492..>5514
                     /locus_tag="VN178_10005"
     CDS             4492..>5514
                     /locus_tag="VN178_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWS81344.1"
                     /translation="MAESTVSTQPAGRRFMRWLLEERIEEVQGPEAREGEEQHAWWQV
                     VCLTGVDYFSTLGYIPGIAVAAAGALSPVATLLIILLTLFGMLPMYRVVAAESPHGQG
                     SIAMLERLLSFWKGKLFVLCLLGFVATSWVITITLSASDATAHVVENPFWPEALRDER
                     VLITLALLAVLGGIFLKGFREAIGIAVAVVGAYLLVNLVVVAVGFYEIATQPQVLADW
                     QSALFREHGSALGMFGVALLVFPRLALGLSGFETGVSMMPLVRGDADDDPERPEGRIR
                     NTRKMLLAAALIMCFYLMTTSFVTTLLIPQSEFSPNGEAQGRALAYVAHEHLGNAFGT
                     IYDISTI"
BASE COUNT         1024 a         1708 c         1786 g          996 t
ORIGIN      
        1 cgcccttatc tggccgccgt gggcctcggc gatctggcgg gctatggaca gccccaggcc
       61 cgcgccgcca tcggagcggg agggatcagc cctgtggaag cgctcgaaga tgaggggcag
      121 ctgatcctcc gggatgccga cgcccgtatc cgagacttcg agcgcgaccc ggtcaccgtc
      181 ctcccgcaca cctaccgtga ccttcccgcc ctcgggcgtg tagcgcacgg cgttgtccaa
      241 gagtatggcg gcgacctgga aggcgcggtc cctatcgaag gtggcctcga cctcaccttc
      301 ggggaggacg cattcgatgg acacctttcc gttgttggcg tccctggtcg cccgcgccgg
      361 ctcctcggcg accgctgcca ggtcgtggcg gccgatcttc agctccgcgc cttcgtcgcc
      421 gcgggcgagg gcaaggaggg attcgatcag ccccttcatg cgctccgatt ctcgttttat
      481 cgcttccacg ctctccggtc ctaactgtgg gtctttcaat gcccactctt tgagcatctg
      541 cgcatagccg tgtatcgagg taagtggagt acgcaactcg tggctggcat cggcggcgaa
      601 tcgtcgctga cgctccaagg actcctgcat gcggacgaag gcggcttcca ggcgcgagag
      661 cagaccgttt atcgtgatga ccagacgccc gatctcgtct ttggggttgg ccacgggcag
      721 ccgcttggat agatctccct cgcccatctt gctggccgcg ttgggtacgg agtccaccgg
      781 cctcagggcg gctcccgcca gctggtaggc gccaccgata gacaggagaa aggccgcccc
      841 gatgacgaac gccagcacgg ttgcgaaaat gtcgaggatc tcctcggttt cgttgtagga
      901 cttgccaaac tcaaccactc gagccgggcg agccgaaggg tccatcgcta cggcgtagac
      961 gtagtagtca ttatccccaa ggcgcatcct cttatcagag gcgtcttgcc cggttttgag
     1021 cgccttgcgc cacacgtcgg aatcggcggt tgagcctccc gccgggagat tgaacttatc
     1081 gatgaccttg ccatttttgc cccggatgat cacggcgacg ctgtcgtcga ataagatctg
     1141 gtcgtcatcg ttgggggcgg tgctcagttg gtctcccgac tcgtaggtgt gggcaacggc
     1201 ttccccccga ctcttggcgg tgacttcgac gtccttgagt agcatccctc tgatcagaaa
     1261 gtagagtgat aggcccagga cgagcagtat caccccgatc accagcgcga tgaacagcgt
     1321 taggcgccac ctgatagaca accgctcatt cccccttcaa agcgtacccc gccccgcgca
     1381 cggtgcggat cagcttctcc tcgccagggg cgtccacctt tctcctgagg tagccgacgt
     1441 agacatccac cacgttgggg gtgaggcccg actcgccatc ccagacgtgc gagagcagga
     1501 gatcgcgggt gagcacccgg cgaggattcc tggccataaa cgcgagtaat tcgtactcgc
     1561 gggtcgttag ctcaatctcc ctctcctccc gtctgacctc ccgggtggag gtgttgatct
     1621 ccaggtcggc tacacggagt atctcgtcac cctcgaccct ccttaacagc gcgcggatgc
     1681 gggcaattag ttcctctatg tcgaacggct tggtcaggta atcgtccgcg ccgcggtcga
     1741 gactgtgtac cttgtcgagc gtggtgtcgc gggctgtcaa catgatcacg ggcaccctac
     1801 tgcccccctc gcgtaaccgc ctgaggaccc cgaccccgtc caaccccggc agcatgatat
     1861 ccaggacgat cagcgcaggt ccgaatctct ccgcctcctt caggccggtg ggaccgtcgt
     1921 acgcgcaccg caccaggaag ccgagatgtt cgagctcggg ttcgacgaag ccggcgatcg
     1981 cacggtcgtc ctccaccaac aatatcctgg tgcccggtct catctactcc ccttgggtta
     2041 cctgatctgt attgtacttc cggatggcca aactgtgcgg gggataacga gacttctcca
     2101 gagcacaccc acaaagctac gggtcttggc accccacgcc gctgtattcc tgcgggtgca
     2161 cgagggttca ctctagacgc cctccctgcg agggaggtga gaacctgcaa tgctgagcgg
     2221 ccccgcatcg gggctcgtac ggtgaggagg ttcttgcgga catgactggg ggttggaagt
     2281 ctgtataatt tcggtccgtc atggccggct tcacgggagt tttctgactt ggatctctac
     2341 gagtatcaag gtaaagagct gttgagggaa tacggcctgg agacgttgcc gggcatcgta
     2401 gcgggaacgc ctgaggaggc ggaaagggcc gccgagactc tcgggggaac agtggcgatc
     2461 aaagcccagg tgctgaccgg ggggcgcggc aaggccggcg gcatcaaggt cgtgaaaagc
     2521 ccgcgggagg ccgaagaggc ggcggagcag atcctcggca tggacattcg cgggcacacc
     2581 gtaggacgag tctacgtcga gcacggagag gagatagaga gggagttcta cctctccatc
     2641 accgtggaca ggacaaagaa gcggcccttg atcctgttct ccacggaggg cggggtggag
     2701 atcgaggagg ttgccgagac aaatcccggg gcgatcgtca ggcgccacgt ggatccactc
     2761 ctcggcctcc ttccctacca ggtgcgggag atcaccttcg ccgcgggact ggaaggtgac
     2821 gtcgcgaagg gcgtgggaat ggtgctctcg cacctgtaca aggccttcga aggtctcgac
     2881 gcgagcctgg tcgaactcaa tcctctcgtc gttaccagag aggggcgtgt ccttacgctc
     2941 gacgcgaagg taacggttga cgattccagc ctgtataggc acgaagactt ggccgggtta
     3001 cacgacgtgg aggccgcaga cccacaagag gagcgggcgc aggaggtcgg gctgcagtac
     3061 gtcaagctcg acggcgacat cggcatcctc ggcaacgggg cggggctcgt gatgagcacg
     3121 ctcgacgtcg tcgcccaggc gggcggcgaa cctgccaact tctgcgacgt cggtggcggg
     3181 gccgacgccg agcagatctc caccgccctt gatatcgtca cctccaacga gcaggtaagg
     3241 agcgtgctct tcaacatctt cggcggcatt acgcgcggcg acgaggtggc gcggggattg
     3301 ctctcggcca tagagcggac ggagatcacg ctccccatcg tcgtgaggct cgacggtacg
     3361 aacgccgaag aaggacgcag gatcctcgcc gagaacacac cggagaacgt ggtcaccgag
     3421 gagacgatgc tggaggctgc cagcagggct gtagagctcg caagagacgg gggaaggtaa
     3481 cttcatggcg attctagtag acgatgggac gcggctgctc gttcagggca taacgggtag
     3541 ggagggcgcg ttccacacgg gaaggatgct ccagagcggc accagggtgg tcgcgggggt
     3601 gacgccgggc aagggcgggc aggactacga gggcgtcccc atcttcgaca cggttggaga
     3661 ggccgtcgga caaaagggcg cgaacgcgag cgtgatcttc gtccccccac ccttcgccgc
     3721 tgacgccatc ttcgagtctt tggacgcggg gctctccacc atatgctgca tcacggaagg
     3781 catccccgtc cacgacatga tgcgggtcgc gaactatctg aagaacaagg acgcgaccct
     3841 cgtcgggccg aactgtcctg gcgtcctctc gccggagaag tcgaacgtct ccataatgcc
     3901 caaggacatc ttcatgccag gcaacgtcgg cgtcgtaagt cgcagcggca cactgaccta
     3961 ccagatagtc aacgagctca cgcagaggga gatagggcag agcaccgccg tcgggatcgg
     4021 cggcgacccc atcataggga cggacttcat cgagatcctg agcaagttcg aggccgatcc
     4081 cgagacggaa tgcgccgtga tgatcgggga gatcgggggc aatgcggaac agctcgcggc
     4141 cgagtacatc agaggcgaga tgagcatccc cgtcgtggcc tacatcgcgg gcttcacggc
     4201 ccctgaaggc aagaccatgg ggcacgcggg ggccatcgtc tccggcggcg agagcacggc
     4261 agaggccaag agcgaggcgc tcagggaggc tggcatcagg gcagcgacga atccatccga
     4321 ggtcgccgag ctggtcgccg aggcactcgg caggagctga aggacagaga atccgcagga
     4381 cgaagaggtc tgacccctag atctccgata ccttatactt tctggactca ccatgaacgg
     4441 gtatagtcca acccaggcaa gcctgatttc accgagcggg aaagtggcgt agtggccgag
     4501 tccaccgttt cgacgcagcc tgccggcagg cggttcatgc gctggctcct ggaggagcgc
     4561 atcgaagagg tgcaggggcc cgaggccagg gagggagaag agcagcatgc gtggtggcag
     4621 gtcgtctgcc tgaccggtgt cgactacttc tccaccctgg gctacatacc cgggatcgcg
     4681 gtcgccgccg cgggcgcgct atcacccgtt gccaccctgc ttatcatact attgacgctc
     4741 ttcgggatgc tgcccatgta ccgcgtcgtc gcggcggaga gcccgcacgg gcagggttcg
     4801 atcgccatgc tcgaacgcct gctctcgttc tggaagggta agctgttcgt cctgtgcctg
     4861 ctcggcttcg tggcgacctc ctgggtcatc accatcaccc tttcggcctc ggacgccacg
     4921 gcccacgtcg tcgagaaccc gttctggccc gaggccctgc gggatgagag ggtgctcatt
     4981 accctggcac tgctcgctgt gctaggcggg atctttctca agggcttcag ggaggctatc
     5041 ggcatcgcgg tcgccgtggt cggggcctat ctgctggtga acctcgtggt cgtagcggtc
     5101 ggcttctacg agatcgcgac gcagccccag gttctggcgg attggcagag cgcgctcttc
     5161 agggagcacg gtagcgctct cggcatgttc ggggtcgccc tcctcgtatt cccccggctg
     5221 gccctcggtc tctccggctt cgagaccggc gtgagcatga tgccgctggt acgcggagac
     5281 gccgacgacg accccgagcg tccagaaggg cgcatacgca atacgcgcaa gatgctcctg
     5341 gccgcggccc tgatcatgtg cttctacctg atgacgacga gcttcgtgac gaccctcctg
     5401 ataccccaga gcgagttctc tccgaacgga gaggcccagg gcagggccct cgcctacgtg
     5461 gcgcacgagc acctgggcaa cgcgttcggc acgatctacg acatcagcac gata
//
LOCUS       DATMVF010000201        11015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_126292, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000201 DATMVF010000000
VERSION     DATMVF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11015
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126292"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VN178_10010"
     CDS             <1..773
                     /locus_tag="VN178_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HWS81345.1"
                     /translation="PHLESALGPLVERGLASTFVSLGNGLVQDRIAAIVGDENLVVGT
                     VEWGATNLGVGHLAQTTRAPFVIGEPDGETKERTRLLAEVLETVADVRITENISGQVW
                     SKLLVNSAFSGLGAVSGLLYSGVVADPAGREAALAVWREGYDVGVAQGITMDEVLGVP
                     ADSLVVRSSEDRERADGALEVAMGYAGATKASMLQDLEKGVKTEVDVINGGVVERGRE
                     YGVETPHNERVVDLMHAMERGERRPGRDSLEELQALAG"
     gene            complement(922..1587)
                     /locus_tag="VN178_10015"
     CDS             complement(922..1587)
                     /locus_tag="VN178_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWS81346.1"
                     /translation="MKRVCVFDVNETLLDLAALDPLFERVFGDAAVRRLWFNQMLQSA
                     FVATITDAYVTFGEAAGAALGMTEERLGVEVSEDDRREILGGLRDLPPHPEVPESLDR
                     LHEAGFRLATLTNSTQEVAEAQIQTAGLTDKFEQILSADTARRLKPAPAPYRMAARSL
                     GVPDRGMRLIAAHAWDVAGALRAGCAAAFVARQPFDPLVEEPDVVGPDLAEVADKIIA
                     AED"
     gene            complement(1749..2672)
                     /locus_tag="VN178_10020"
     CDS             complement(1749..2672)
                     /locus_tag="VN178_10020"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HWS81347.1"
                     /translation="MEKFIISAAITGGMTVPGQSEAIPITPDEIIDSAVGAHEAGATI
                     VHLHVREPETGKPSSDIGLFREVVAGIKDRCDVIVQPTTGGGKGMTLEERAAVLPELE
                     PEMATFNTGSFNFGLFPIAERGGDYEEWEIDYLEDTRDYIFRNTFADMEKVCTMFREA
                     GAKPELEAYDVGHLYNIKYLLGRDLLDTPVHIQFVLGVMGANAATIEQMMHMRRTATE
                     LFGDDYTWSAAGVGYPAEYHLAAMCLMLGGHVRVGLEDNLRVSREKRAESNAELVEKA
                     VALGAMFDREPATPDEAREAFGLKGRAEVAF"
     gene            2897..3175
                     /locus_tag="VN178_10025"
     CDS             2897..3175
                     /locus_tag="VN178_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81348.1"
                     /translation="MEKTVKGEAAVGIQPETVGAHASQGIGPISAIGAALHNLIRLRT
                     RKKISAEAIGEPEQVRRPGHSVSRSPMDAGITGGISLFLDKPNARRRR"
     gene            complement(3235..4764)
                     /gene="asnB"
                     /locus_tag="VN178_10030"
     CDS             complement(3235..4764)
                     /gene="asnB"
                     /locus_tag="VN178_10030"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HWS81349.1"
                     /translation="MCGIIGGYGGMDSESVSRMLSRVVHRGPDDEGRIEVAGNVLGHR
                     RLSIVDVEGGKQPLFTPDGGLYLVGNGEVYNHEEIRETLEDGEMGTRSDNEVALRLIS
                     ERGPGALSELLGMFAFLIAGEDGTFIAARDPVGIKPLYWARRDGLVRFASEMRSFDED
                     WQSDVESFPPGHYWTPEGGLVEFATPVPPRDELERFSGPDEPGARIPDEILEKVRSRL
                     VRTVGRQMMGDVPVGVFLSGGLDSSLVAAIAARWYEERGEKLETFTVGLADSPDLLAA
                     RAVAEYLDTEHHESVYTADDALAALPKVVRTIESFDPSLVRSAVPNYILAAFTARHVK
                     VVLTGEGADEIFAGYEYLRDFRTEEELHAELVRTIEGLHDLNLQRADRVTMAHGLEAR
                     VPFLELDMISLGLSLPAGWKLAGEGQEEKRLLRQAFKGWLPDEFLWRKKAQFGDGSGA
                     ASVLKALMEESVSEEEFRRERHEVEPPLRTREEVAYYRIFAEDLGEVSPDRTIGRFAT
                     T"
     gene            complement(4827..5096)
                     /locus_tag="VN178_10035"
     CDS             complement(4827..5096)
                     /locus_tag="VN178_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81350.1"
                     /translation="MLRSGCLSLVVRVGVVSVAAILAALSMVVILSFAVSALGQPDGV
                     EKIVKVLVASAAGFAFFLVGRGIWRDLRRRSTAKPEEKPTKERGR"
     gene            5582..6457
                     /locus_tag="VN178_10040"
     CDS             5582..6457
                     /locus_tag="VN178_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HWS81351.1"
                     /translation="MDTIGITSGRRNERPAETPDLIPGYFARIDKGNLLTHREEIDLS
                     KRAKAGDDRARKKLVEKNLRLVVSVAKRYRGMGLPFEDLIQEGNVGLMRAVEKFDPDR
                     GWRFSTYATWWIRQAVQRAVADKGRTIRVPVHMGDKIRKMARAYNELSTELSREPTDE
                     EVAGRLEWTVGEVLDVKSAMPDATSLNQTLSSDGDGSELGELIEDERASEVAGTVIGE
                     MEVGSLKEAIQRLPERHRHVLVRRYGLGDRDTATLAKLSEELGISRERVRQLQREAER
                     MLGSGELGMVLRGAA"
     gene            complement(6630..7481)
                     /locus_tag="VN178_10045"
     CDS             complement(6630..7481)
                     /locus_tag="VN178_10045"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWS81352.1"
                     /translation="MDERRIRVMSFNVRGSFRDARRKNAWRNRVELNVATIERYVPDV
                     IGLQEAQRGNLSAYRKRLPRYAQIRGPRYGNAIPHDFNTILFDPERLEPLDSGGFWLS
                     ETPEKHSRSWETRVARSATWALFEAPGTNLTLLHLNTHLDHISALARQEGSKLLVRKV
                     AEISGRTDVDPPAIVTGDFNSRPGNRVHNTFTESGFVDSYLAAGNEEGANTFHAFKGA
                     LYHDAHPARGPRRIDWILLKDPEGRLRIDSHKIIHDADEDSGLYPSDHYPIMADFGIS
                     ASQHAPA"
     gene            7606..8079
                     /locus_tag="VN178_10050"
     CDS             7606..8079
                     /locus_tag="VN178_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWS81353.1"
                     /translation="MDWDRRNPFHGLVDSISEWNRMREVGSGRIGPEAGHEGRRRTYA
                     TAWVPSTDVVARGRDLVIRVSLSGVSPEDVEITLSSDVLTVSGERRGDPDDQDETFYV
                     RERYYGVFMRSWTLPAGIRADDVSADFENGLLEITVKGGAADVPEPRRIEIKRRP"
     gene            complement(8083..8520)
                     /locus_tag="VN178_10055"
     CDS             complement(8083..8520)
                     /locus_tag="VN178_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HWS81354.1"
                     /translation="MHFDLHELTRLDLLRRGRRCWDHRSGQQVSRRRTEPEPADQAHL
                     GRHNGALAAILLLAGGLDALQNGAILAATPFAILMCLVCWCLYKTLRRDYREERQQVQ
                     EIMAHDQNVEKQQMEEIMRRHEAGEPVGQAVSDSTNGESRKGS"
     gene            8739..9950
                     /locus_tag="VN178_10060"
     CDS             8739..9950
                     /locus_tag="VN178_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWS81355.1"
                     /translation="MRIPYDTPRIHMYTRIRKGPFFYAHRRHGVQSYTVTNRQYHPRH
                     YDDMLVEYWKLVNDVTMWDVAAERQVEITGPDAFEFTNMLTPRDLNKCSVKQCKFALN
                     TDVDGGIINNPVLLRLGENHFWLSVADGDVLLWAKGVAAVCGLDVQIREPDVAPVQIQ
                     GPRSRDLMVDIFGEGILDLQYYWMDEYDLDGMDVVVSRTGYTGEVGFEIYLRNASRDG
                     MKFWDTMLEAGEPHDLSPIGPCQIRRVEAGILSYGSDIALDNNGYSDYHFLNPYEADL
                     GYTVELDQEADFIGKEALRRIADEGVDRKVVGIELDCDPLIGYIEDYLQVVDNGSQIG
                     QVSSAFWSPRLEKNIGYALVPIELADLGTGLTVRMETGEYEATVVEKPFIDPRKETVK
                     ANLKLGTARSY"
     gene            10182..>11015
                     /locus_tag="VN178_10065"
     CDS             10182..>11015
                     /locus_tag="VN178_10065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS81356.1"
                     /translation="MQLPTQASYVVIGAGVHGLSTAWHLARELESRGRGSGEDVIVLD
                     KTGVAAGASGIACGVIRNNYFQPAMRKLMAHSVSVWESDPEEFAYHPVGYMQISPEVM
                     HDDVVQIYKEQQAIGYPSTLVEGEEECRSYMRSIFHDWRAENITSILHEHRGGYANNK
                     ASVRALAAKARAEGVRIHTGVRVTGFRMEGGSVNAVQTDGGVVGCEHLVVAVGPWVRD
                     IWAMLDLPEKITVKGPDGGLYCDREMWTYWSMQEGVLGVEPSFLTDNEGGMPPVIHVD
                     TD"
BASE COUNT         2082 a         3424 c         3554 g         1955 t
ORIGIN      
        1 cgccccacct cgaatccgcc cttggtcctc tggtagagcg aggtctggca agtacgttcg
       61 tctctttggg caacggcctc gtgcaggacc gcatagccgc gatcgtcggg gacgagaacc
      121 tcgtcgtcgg aacggtggag tggggggcga ccaatctggg agtcgggcat ctagcgcaga
      181 ccacgcgggc gccgttcgtg atcggggaac ctgacggaga gacgaaagaa aggacccgcc
      241 tgctcgccga agtactcgaa acggtggccg acgtacgtat cacggagaac atcagcggcc
      301 aggtgtggtc aaagctgctc gtcaacagcg ccttctccgg tctcggcgcc gtctccggcc
      361 tcctctacag cggggtcgtc gccgaccccg cagggaggga ggcagcgctc gccgtctggc
      421 gcgaaggcta cgacgtcggg gtggcccaag ggatcacaat ggacgaggtc ctcggcgtcc
      481 ccgccgattc gctagtcgtg cggagttccg aggatcggga gagagccgac ggagccctcg
      541 aagtggcgat gggctacgcc ggggcgacca aggcttcgat gctccaggac ctcgagaagg
      601 gtgtgaagac cgaggtcgac gttataaacg gcggcgttgt cgagaggggt agggagtacg
      661 gcgtcgagac cccgcacaac gagcgggtcg tcgacctgat gcacgcgatg gagcggggcg
      721 agcggcgtcc gggccgggac tccttggaag agttgcaggc tctcgccggt tagagcactt
      781 cagcacgctc cagcctgcgg ctgtcgcttg tcagcatttc agcatttcag cttgaatatg
      841 tccaagacct ggctgacatg ctgacaaggc ggcgcagccg ccgggctgac aagccgtcga
      901 agacggcgtg ctgaattgct ctcaatcctc ggcggcgatg atcttgtcgg cgacctcggc
      961 caggtcggga ccgaccacat ccggctcttc tacgagcgga tcgaaaggct gcctggcgac
     1021 gaaggccgcg gcgcatcccg cacgcaaggc ccccgcaacg tcccaggcgt gggccgcgat
     1081 caggcgcatc ccccgatcgg gcacgccgag cgaccgcgcg gccatcctgt agggggccgg
     1141 ggcgggcttg aggcgtcttg cggtgtcggc ggagaggatc tgctcgaact tatcggtgag
     1201 ccccgcggtc tgtatctggg cctcggcgac ctcctgcgtg gagttggtaa gggtggcgag
     1261 acggaacccg gcctcgtgca gtctgtcaag gctctcgggc acttctgggt gtggcggcag
     1321 gtcgcgcagt ccgcccagga tctcccgccg atcatcctca gacacctcca cccccaaccg
     1381 ctcctcggtc atccccaggg cggcgcctgc ggcctcgccg aaggtcacgt aggcgtccgt
     1441 aatggtggcc acgaaggccg actgcagcat ctggttgaac cacagcctcc gcacggcagc
     1501 gtcgccgaag acccgctcga agagcgggtc tagagccgcc aggtcaagca acgtctcgtt
     1561 cacatcaaag acacaaactc tcttcacaat actcacatct ccaaaacgat tcgcagagat
     1621 atcagccaac cgtaggtcgc gcttgcgagc cttggctgac aaggcggcgc agccgccggg
     1681 ctgaaatggt gacaagcgaa gcccgaaggg ctgagcgtgc tgagatgctg aaccgctgcc
     1741 aagcggttct agaacgctac ctcggcgcgg cctttgaggc cgaacgcttc gcgcgcttca
     1801 tcgggggttg ccggctcgcg gtcgaacatc gctccgaggg caacggcctt ctcgaccagc
     1861 tcagcgttcg actccgcgcg cttttcgcgg ctgacgcgga ggttgtcttc caggccgacc
     1921 ctcacgtggc cgccgagcat caggcacatc gcggcgaggt ggtactcggc cgggtaaccg
     1981 acgcctgccg ccgaccacgt gtagtcatct ccgaatagct cggtcgccgt gcggcgcatg
     2041 tgcatcatct gctcgatggt cgcggcgttc gcgcccatca cgccgaggac gaactggatg
     2101 tggaccgggg tgtcgagcaa gtccctgccg agcaggtact ttatgttgta gaggtgtccg
     2161 acgtcgtagg cttccagctc cggcttcgcg ccggcctcgc ggaacatggt gcagaccttc
     2221 tccatgtcgg cgaaggtgtt gcggaagatg taatcccggg tatcctccag gtagtctatc
     2281 tcccactcct cgtagtctcc gccccgctcc gcgatcggga agaggccgaa gttgaagctg
     2341 cccgtgttga aggtggccat ctcgggctcc agctcgggca ggacggcggc ccgctcctca
     2401 agcgtcatgc ccttcccgcc gcccgtggtc ggctgtacga tcacatcgca ccggtccttg
     2461 atccccgcga ccacctcgcg aaacagcccg atgtccgagc tcggcttccc ggtctccggc
     2521 tcgcggacat ggaggtggac gatcgtcgcc ccggcctcgt gcgcccccac agctgagtcg
     2581 attatctcat ccggcgttat aggaatagcc tcactctgcc ctggcacggt catcccgccc
     2641 gtaatcgccg cgctgataat gaacttctcc acacccgctc ccttctttag agtccttact
     2701 cgatactaga tacccaccag gaccgcagct aaccggaccc ggcgcccggc cgcggaaaaa
     2761 atgacagccc tcttccggaa aaaaatcgaa catccgcgcc acaaatactg caaatgagtg
     2821 atgcgctggc atcccggatc ggtcatgctc atgtggtagt gccagagcac gcaagaactg
     2881 cggaaagaag gcagaggtgg agaagacggt aaagggcgaa gcggcagtcg gaattcagcc
     2941 agagacggtc ggtgctcacg cgtcgcaggg gatcggcccg atctccgcga taggcgccgc
     3001 cctacacaac ctgatccgtc tccggacgcg caagaagatc tcggccgagg caatcggcga
     3061 acccgagcag gtgaggagac caggacacag cgtgtcacgg tcccccatgg acgcaggtat
     3121 caccgggggt atttcgctgt tcctcgacaa gccaaacgcc cgcaggcggc ggtgacacat
     3181 cctgaaacct gataccggag ttcagggaag gcaaaaacct tccctgaact cttttcacgt
     3241 tgttgcgaag cgtccgatgg tccgatccgg gctgacctcg ccgaggtctt cggcgaagat
     3301 gcggtagtag gcgacctcct cgcgggttcg caagggaggc tcgacctcgt gccgctcgcg
     3361 cctgaactcc tcctcgctga cgctctcttc cataagcgcc ttgaggaccg aggcggcccc
     3421 cgatccatcc ccgaactgtg ccttcttgcg ccacaaaaac tcatcgggga gccagccttt
     3481 gaaggcctgg cggaggagcc tcttttcttc ctggccctcg ccggcgagtt tccaccctgc
     3541 cgggagggag agtccgaggg agatcatatc gagctccagg aacggtaccc gcgcttcgag
     3601 gccgtgggcc atggtgacgc ggtcggcccg ctgcagattc aggtcgtgca acccctcgat
     3661 ggtccgcacc agttcggcgt gtagctcttc ctccgtgcgg aaatccctca ggtactcgta
     3721 gccggcgaag atctcatcgg ccccttcgcc cgtcagaacg accttgacgt gcctggccgt
     3781 gaacgccgcc aggatgtagt tgggtacggc gctacgtacc agcgaggggt cgaagctctc
     3841 gatggtcctg actaccttgg gcaaagccgc gagagcgtca tcggccgtat acacgctctc
     3901 gtggtgttcg gtgtcgaggt actcggcgac cgcgcgggcg gccagcaggt cgggagaatc
     3961 tgcgaggccg acggtgaagg tttcgagctt ctcgccgcgc tcctcgtacc agcgggccgc
     4021 gatggcggcg acgaggctgg agtcgaggcc gccggagaga aagacgccga cgggcacgtc
     4081 gcccatcatc tggcgcccga cggtcctcac gagccggctg cggaccttct ccagtatctc
     4141 atccggaatc cttgcgcccg gctcgtcagg cccgctgaag cgttccaact cgtctctggg
     4201 cgggacgggt gtggcgaact ccacgaggcc gccttcgggg gtccagtagt gacccggcgg
     4261 gaaggattcg acgtcggact gccagtcctc gtcgaaggat ctcatctcgg aagcgaagcg
     4321 gacgaggccg tcgcggcggg cccagtacag aggcttgatg ccgacagggt cgcgggccgc
     4381 gataaacgtg ccgtcctccc cggcgatcaa gaacgcgaac atcccgagca actcagagag
     4441 ggcgccgggt ccccgctcgg agatcagacg cagcgccacc tcgttgtccg agcgggtgcc
     4501 catctcgccg tcttcgaggg tctcccggat ctcctcgtgg ttgtatacct cgccgttgcc
     4561 gaccagatag aggccgccgt ccggcgtaaa gagcggctgc ttgcctccct ccacatcgac
     4621 gatggagagc cgcctgtggc cgagcacgtt ccccgccacc tcgatgcgcc cttcgtcgtc
     4681 cggccccctg tgtacgacgc gcgagagcat ccgcgaaacg gactcagagt ccatgcctcc
     4741 atacccgcca ataatcccac acatctcact ccgttctact ccgggcgcgg tacactcgtc
     4801 gtccctggag cgaatctccc tacccgctag cgcccgcgct ctttggtcgg cttctcttcg
     4861 ggttttgcgg tcgaacgtcg cctcaggtcc cgccagatgc cacggccgac caggaagaag
     4921 gcgaaccccg ctgccgaggc cacgagcacc ttgacgatct tctcgacccc atccggctgc
     4981 ccgagcgccg aaacggcgaa cgagaggatg acgaccatgc tcaacgcggc gaggatcgcg
     5041 gccaccgaca ccacgcctac ccttaccacc aggctcaagc accccgaacg caacatgttc
     5101 ctctcattgc gctccgtgga aggttcaacc ttccacggag cgcgccggtc agcatttcag
     5161 cacttcagct tgtcagcctt tttgctgaaa gctgatggct cggtgtacaa aaggttcgag
     5221 acgtttgtgt acggcgctat cagtgcctga gtctctgcag cttcgtccat cgccagatac
     5281 tatactccct aaagcaccgc ttgggtcgga gcctttgccg tcggccgccg gtggctgacc
     5341 gcgaaaacca ccaggccgat acccaaggcc aaactggcct tgacaaacgc tccgaaatga
     5401 gtatactgct agtttggcaa gatcctaaaa aaagcctgca ttcccgcacc catacaactt
     5461 cgagcaacga agggcggcgt ccgcgcggcg cgtttttgtg ggttcgggac gcccgggagt
     5521 ggggacgtcg tgcaggattt tgagggcgta gacggtttat acacgagtaa ggaggcgttt
     5581 gatggacacc attgggataa ccagcgggcg gcgcaacgag cggcctgcgg agacccctga
     5641 tctcatcccc gggtatttcg cgcgcatcga caaggggaat ctcctgacgc acagggagga
     5701 gatcgacctc agcaagaggg ccaaggccgg tgacgacagg gcgcgcaaga agctcgtcga
     5761 gaagaacctg aggctcgtgg tcagcgttgc caagaggtat cgtgggatgg ggctgccgtt
     5821 cgaggacctt atccaggagg gcaacgtcgg ccttatgagg gccgtcgaga agttcgaccc
     5881 cgatcgcggc tggaggttct ccacgtatgc tacctggtgg atccgccagg ccgtgcagcg
     5941 tgccgtggcg gacaagggcc gaaccatccg cgtccccgtg cacatggggg ataagatccg
     6001 gaagatggcc cgtgcctaca acgagctctc caccgagctg agccgcgagc ccaccgatga
     6061 ggaggtcgcc ggacgcctgg agtggaccgt cggcgaggtg ctcgacgtca agagcgcgat
     6121 gcctgacgcc accagcctca accagaccct ctcctccgac ggcgacggct ccgaactcgg
     6181 agagctgatc gaggacgagc gtgcctctga ggtggcgggc acggtcatcg gggagatgga
     6241 ggtaggctcc ctcaaggagg cgatccagcg gctccccgag cgtcacaggc acgtcctggt
     6301 gcggcgctac ggcctcggcg accgcgacac cgcgaccctc gccaagctaa gcgaggagct
     6361 cgggatctcc cgcgagcgcg tccgacagct gcagcgcgag gccgagcgta tgctcggcag
     6421 cggagagctc ggtatggtcc tacgcggcgc cgcataatgc gaattggggc ggccctggcc
     6481 gccccaattc gcgcatttca gcacgctcca gcctccggct gtcgcttgtc agcacttcag
     6541 cttgtcagcc tttgcatggg cagatcctgg ctgaaatgct gacaagcgcc caacagggcg
     6601 cgtgctgact agctgacaag cggaggccgc taggccggag cgtgctgact ggctgaaatg
     6661 ccaaaatcgg ccattatcgg ataatggtcg ctcggataga gtcctgagtc ctcatcggcg
     6721 tcgtggatta tcttgtgcga gtctattcgt agtcgccctt cggggtcttt caggaggatc
     6781 caatctatgc gccgcgggcc gcgggcgggg tgcgcatcgt ggtagagcgc tcccttgaat
     6841 gcgtggaacg tgttcgcgcc ttcttcgtta ccggcggcga ggtagctgtc tacgaagccc
     6901 gattcggtga acgtgttgtg gaccctgttg cccggcctgc tgttgaagtc tcccgtaacg
     6961 atggccggcg gatctacgtc cgtgcgaccg gagatctcag cgaccttcct cacgaggagt
     7021 ttgctacctt cctggcgtgc cagggcgctt atgtggtcga ggtgcgtgtt caggtggagg
     7081 agagtgagat tcgtccccgg cgcttcgaac agtgcccagg tggccgagcg cgcgaccctc
     7141 gtctcccagc tcctcgagtg cttctcgggt gtctccgaga gccagaaacc tcccgagtcg
     7201 agcggctcca gccgttcggg gtcgaagagg atcgtgttga agtcatgggg gatggcgttg
     7261 ccgtatctgg gaccgcggat ttgggcgtag cgcggcagac gcttgcggta tgcagagagg
     7321 ttgccgcgct gggcttcctg aagcccgatc acgtcaggga cgtagcgctc tatggtggca
     7381 acgttcagct cgacccgatt gcgccaggcg ttcttccttc gcgcgtcgcg gaacgacccc
     7441 ctgacgttga agctcataac ccggatccga cgttcgtcca cgcggaggtc ccccaaggct
     7501 cgccaacgac agctccatcg attgtagccg gtgcgagcgg gccggttttc gttgcactag
     7561 agtcgggtaa agcgcaagtc ggaaaagcat tcgaaaggag agaggatgga ctgggaccga
     7621 agaaacccgt ttcacgggct tgtggactct ataagcgaat ggaaccggat gcgggaggtg
     7681 ggctcgggga ggatcggccc cgaagccggg cacgagggcc ggcgcaggac ctacgctacc
     7741 gcctgggtcc cttccaccga cgtggtcgcc aggggtagag acctcgtgat ccgggtctcg
     7801 ctctcgggag tctctccgga ggacgtagag atcacactct ccagcgacgt gctcaccgtc
     7861 tcgggagagc gcaggggcga tccggatgac caggatgaaa cgttctacgt cagggagcgt
     7921 tactacggcg ttttcatgcg gagttggact ctgcccgcgg gaatacgcgc ggacgatgta
     7981 agcgccgact tcgagaacgg cctgctggag atcaccgtca agggcggcgc agcggacgtc
     8041 cccgagcccc ggcgcataga gataaagcgt cgaccgtaag gttcaggagc ccttcctcga
     8101 ctcgccgtta gtggagtccg acaccgcctg gccgacgggt tctccggcct cgtggcggcg
     8161 catgatctcc tccatctgct gtttctcgac gttctggtcg tgggccatta tctcctgaac
     8221 ttgttgtcgc tcttcgcggt agtcgcgcct gagcgtcttg tagaggcacc agcacacaag
     8281 gcacatcagt atcgcgaacg gcgtagccgc caggatcgcc ccgttctgca gcgcgtcgag
     8341 acccccggcc aggagcagta tcgccgcgag cgccccatta tggcgcccca ggtgagcttg
     8401 atcagccggt tcgggttcag tacgccgccg gctgacctgc tgcccagaac gatggtccca
     8461 gcatcggcgt ccgcgacgaa gaagatctag acgagtaagt tcatggagat cgaagtgcat
     8521 tcgggggcgc aggttacaga cgcactttcc cgaatacaac ccgctggtct atccaccggg
     8581 tttcagatac agcaactacg gttgcgtgct caatttgccc gtgatacatt ccggcggcag
     8641 agggagaaaa actggaggac gataccggat tactcgcagc gaagcacccg gattccgacg
     8701 aacagggtta cgatcacgac gttcagggag gtagtctctt gcgcattccc tacgacactc
     8761 cgaggatcca catgtacacg cggatccgca aggggccgtt cttctacgcc caccggcgcc
     8821 acggtgtcca gtcctacacg gtcacgaacc gccaatacca ccccaggcac tacgacgaca
     8881 tgctcgtgga gtactggaag ctcgtcaacg acgtcaccat gtgggacgtg gcggccgagc
     8941 ggcaggtcga gatcaccggt cccgacgcgt tcgagttcac caacatgctc accccgcgcg
     9001 acctgaacaa gtgctcagtc aagcagtgca agttcgccct aaacaccgat gtcgacggcg
     9061 gcatcatcaa caacccggtg ctcttgcgtc tcggtgagaa ccacttttgg ttgtcggtgg
     9121 ccgacggcga cgtgctgctc tgggccaagg gtgtggccgc ggtctgcggt ctggacgtgc
     9181 agatccggga gcccgacgtc gccccggtac agatccaggg tccgagatct agggacctca
     9241 tggtggacat cttcggagag gggatcctcg acctccagta ctactggatg gacgagtacg
     9301 acctcgacgg catggacgtc gtagtctcgc ggaccggata cacaggcgag gtggggttcg
     9361 agatctacct cagaaacgcc agccgcgacg ggatgaagtt ctgggacacg atgctcgagg
     9421 ccggcgagcc ccacgacctc tcgccgatcg ggccttgcca gatccggcgg gtcgaggccg
     9481 gaatcctgag ctacggctcc gacatcgcgc tggacaacaa cggctacagt gactaccact
     9541 tcctgaaccc ttacgaggct gacctggggt acacggtgga gctggatcag gaggccgact
     9601 ttatcggcaa ggaggcgctc agacgcatcg cggatgaggg tgtggaccgc aaggtggtcg
     9661 gcatagagct cgattgcgac cccttgatag gttacataga agactacttg caggtcgtcg
     9721 ataacggctc gcagataggg caggtctcct cggcgttctg gtctccgcgc ctcgagaaga
     9781 acatcgggta cgccctggta ccgatcgagc tggccgatct ggggacgggg ctgacggtga
     9841 ggatggagac cggcgagtac gaggccaccg tcgtagagaa gccgttcata gatccgagaa
     9901 aggagaccgt taaggcgaac ctgaaacttg gcacggccag gtcgtactag cggcccgaga
     9961 cgttcaccga tgagtgattc cgttgacccc ggaacctcag atccatcgcc caagggagcg
    10021 cccacggact ggacactccc ggtcagggag gttagggtgg ggggcgggtt tgcctaccta
    10081 tctgcgggga cacgcgcacg atgccgggcc ttggcgctcc tgccgtcgct gagggcgtag
    10141 acatagatga gaatggcgag atagtggggt tgttctagaa gatgcagcta cctacgcaag
    10201 catcctacgt cgttatcggg gccggggttc atggtctgag cacggcctgg cacctggcaa
    10261 gagaattgga gagcaggggg cgcggctcgg gcgaggacgt gatcgtgctc gacaagaccg
    10321 gcgtcgccgc tggggcctcg gggatcgcct gcggtgtgat ccgcaacaac tacttccagc
    10381 cggcgatgcg caaactgatg gcccattctg tctcggtctg ggagtcggac ccggaagagt
    10441 tcgcctacca tcctgtcggc tacatgcaga tctcacccga ggtgatgcac gacgacgtgg
    10501 tgcagatcta caaagagcag caggccatcg gctacccatc taccctggtc gagggagagg
    10561 aagagtgccg ctcctacatg cggagcatct tccacgactg gcgggccgag aacatcacga
    10621 gcatcctgca cgagcaccgc ggagggtatg cgaacaacaa ggcttcggta agagccctcg
    10681 ccgccaaagc ccgggccgag ggagtgcgga tccacaccgg ggtaagggtt accggattcc
    10741 gcatggaagg cggcagcgta aacgccgtac aaacggatgg gggcgtggta ggctgcgagc
    10801 acctcgtcgt agccgtcggg ccgtgggtca gggacatctg ggcgatgctg gatcttcccg
    10861 agaagatcac cgtcaagggt cccgatggcg ggctgtattg cgaccgtgag atgtggacgt
    10921 actggtcgat gcaggagggt gtgctcggtg tcgagccgag cttccttacg gacaacgaag
    10981 gcggcatgcc gccggtgata catgtagaca ccgac
//
LOCUS       DATMVF010000202        39199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_305864, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000202 DATMVF010000000
VERSION     DATMVF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 39199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39199
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305864"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VN178_10070"
     CDS             <1..320
                     /locus_tag="VN178_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS81357.1"
                     /translation="IDQIRSQASRRRTQERVEASAPRSQPSEAFAETWRNSQRDQVRE
                     ALNTLPSEQLKILELAYFSGYTHVEISDLLDLPLGTVKGRMRLGLKKIRDYFESRSAA
                     VPR"
     gene            317..1084
                     /locus_tag="VN178_10075"
     CDS             317..1084
                     /locus_tag="VN178_10075"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWS81358.1"
                     /translation="MSEMNNERFEDLKDAYVLGALPEEERLSFEDYLATHPERQAEID
                     ELGAVAGLLAFSPQEQESSPELRSRIMEVVEAEAEPRRDTRRSVSASIGDYLSVRSLA
                     LGAAALLVIGLLSWNVLLQGQVSDLQGQVRNAQGQVEDLQAQVEDAQAQQSQTIKLEG
                     SWAEQGATAEVASMEENEIVLVARNMPSVPDDRTCQIWVIKDDVPKPSGLFQPDGNGT
                     ATPITNSITNADVIAVTVEPAGGSEQPTSDPVLLAEL"
     gene            1333..1539
                     /locus_tag="VN178_10080"
     CDS             1333..1539
                     /locus_tag="VN178_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWS81359.1"
                     /translation="MSVARVTELSAVSETGFEDAIRQGVARATSTLRGVEGAWIKDMN
                     VLIEDGNVTGYKVNMEVTFVLEDS"
     gene            1744..2616
                     /gene="uvsE"
                     /locus_tag="VN178_10085"
     CDS             1744..2616
                     /gene="uvsE"
                     /locus_tag="VN178_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HWS81360.1"
                     /translation="MIRLGYSTQNLTIQASTNRTLRLASLVDAERVKGLVRENIADLK
                     RILRWNAEHGVGLFRMGQNLVPFASHPAFPYDWKAEHGAALSEAGELARDLGLRLSMH
                     PGQYINPGSPKPEVVERSLKELRYVTRVFDLLGSPDGVAVLHLGGAYADKQGSAARFI
                     GVMRSEARVLRYLALENDERVWTVAEVTRTADALGIPAITDAFHHELNPGDLTLEEAL
                     DLSLPTWTTRGVRPKLHLSSQDPAKRAGAHAYSVDSRDWQVLLGAIGGRKADVMVEAK
                     GKEHALAPLGVEIV"
     gene            2652..3089
                     /locus_tag="VN178_10090"
     CDS             2652..3089
                     /locus_tag="VN178_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWS81361.1"
                     /translation="MHANPDLREIRDILQRSGRVAVVGLSDRPYRTSHRIAGALQGFG
                     FKIFPVNPNLRGPVLGEEPYESVQDIPTAVDIVDVFRRSGKVMPVAEDAVAAGAKVLW
                     MQSGVINEEAAAYAEEHGLTVVMDRCIKVDYATLVGSATSPRT"
     gene            3128..3610
                     /locus_tag="VN178_10095"
     CDS             3128..3610
                     /locus_tag="VN178_10095"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWS81362.1"
                     /translation="MGYFGGSPLVMFFYGTLKRGERNHEHFCGGALRVEEGAVRGELF
                     DVHLGYPALVVPEESICAFGTGDYRRDAEEQQRLGRGQAPLLEGPRVFGEIFAFDDPE
                     SRLPDLDRLEGFDPADASSHYRRVLLPVETDEGSGLLAWAYVVEQSSGSHLPGGRWPP
                     "
     gene            complement(3643..4419)
                     /locus_tag="VN178_10100"
     CDS             complement(3643..4419)
                     /locus_tag="VN178_10100"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWS81363.1"
                     /translation="MFTGNPDSGTQITNSRRLIQEEIVASEDRLRELEEKYEDYKVYD
                     NQGERIGKVDDLFVDETDREEYIGVKMGFFGLKSTLIPMDIVRVNEGERTIEVAESKA
                     NVKDAPSFDDDEDITAEYEDRIRSHFGLESVGTFPRGGYGAYSGGGDYADRTREPQDF
                     EAEVDSTDYDRTSADPEYGERAEPTERGDDPRDTTGYMRGASEGRSSMGSTDPFTDET
                     PTTRQTEETETFREGGRSKIRRRVIREEIIEEDDAPDTRS"
     gene            4497..5975
                     /locus_tag="VN178_10105"
     CDS             4497..5975
                     /locus_tag="VN178_10105"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HWS81364.1"
                     /translation="MVSRESGGARILSVDVGSSSVRAELYDDAGNGVAGTEVSLDYEF
                     EYTPEGGAVKDGDELLDLVARAVDGTLSKAGGTEISGVAMSTFWHSVLGVDRDGRPTT
                     PILTWADRRAAECVPDLRERLDETANHRRTGCVPHSSYWPAKLLWSGCAMPETFEKTE
                     RWVSPADYFYARFFGEPYQVGTSMASGTGLFDQNRRKWDRKTLKVLPAEEGQLSAISD
                     EPRSGLAGEWAERWPALRDTPWLPAAGDGACSNVGSGCTGSDRLALMVGTSGAMRVLW
                     KAESVEIPDGPWCYRADAQRYVMGGALSDGGNLIEWLRNTLRLPDPEKTERLLSEMEP
                     DSHGLTFLPLLAGERGPGWADRANGTVSGLSMSTKPVEILRAAMEAVAFRFAIIAEML
                     ERASPGEKEVIASGGGLIHSPTWTRIMADTLGRPVTLSGVKEASSRGAALIALEALGG
                     PEIEAADVPLGETFEPDPARHEVYMKALGRQRRLYDAVLGGA"
     gene            complement(6146..7483)
                     /locus_tag="VN178_10110"
     CDS             complement(6146..7483)
                     /locus_tag="VN178_10110"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWS81365.1"
                     /translation="MTEESQSTGNGRGTTTTGTESLSVTDNRTGKSYDVEIKDGTVRA
                     MDFREIKVDEDDFGLMTYDPGYSNTASCRSAITYIDGEKGILEHRGIPIEQLCENSSY
                     LEVAYLLIYGWLPSEDELNQWTYEITHHTYVHENIKNFMNGFRHDANPMGMLQASVGA
                     LSTFYPEAKEIDNDYQRHVAAVRLIAKVPTLAAFAYRHSLGLPYVYPDNDLSYAGNFL
                     SMLFKMAEPKYEPDPRLEKALDVLFILHADHEQNCSAAAVRVVGSSRVDPFSAIAAGC
                     AGLYGPLHGGANVAVLRMLQRVGSIENIPAFLQGVKQGDERLMGFGHRVYKNYDPRAR
                     IIRGHVDEVLEATNKESPLLDIAIELEKHALDDDYFTSRKLYPNVDFYSGLIYEALGI
                     PTDAFTVMFAIPRTSGWMAQWMEMIDDPELRIARPRQIYTGPRDVDYVPIDER"
     gene            7689..8105
                     /locus_tag="VN178_10115"
     CDS             7689..8105
                     /locus_tag="VN178_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWS81366.1"
                     /translation="MKTDSRNYGIQHDYGPRLEASRAGVMSVGGERTWSVLAHLSTFL
                     NLFTGFLGPVAAFVIWLVYKDDSPTVARHALRSIFYQVAWLTAIFIGWTVTFALMAIL
                     VGFLLVPIMAVVTLAPFVQASYEAYMAYRGGSRRYY"
     gene            complement(8148..8357)
                     /locus_tag="VN178_10120"
     CDS             complement(8148..8357)
                     /locus_tag="VN178_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWS81367.1"
                     /translation="MSIARVTELSATSETSFEDAINVAVARATKTLRGVEGAWVKDMN
                     VSIEDGNIKAYRVNVEVTFQLEDPS"
     gene            complement(8442..9491)
                     /locus_tag="VN178_10125"
     CDS             complement(8442..9491)
                     /locus_tag="VN178_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HWS81368.1"
                     /translation="MAAHDIAVIGASAGGVEALIRLAGALPQDLPMAVFVVVHYPEEA
                     PNTLPHLLSRAGPLAATNPRDGDPIEHGRIYVAAPGSHLQVKEGWVRLTRGPKENYHR
                     PAIDPLFRTAALAYGARVVGVVLSGADGDGTAGLQAVKQRGGVAIVQDPHEASFARMP
                     KSALEYVEVDHCLPLDGIAALLSRLATETRSKEGVEQGGFLMSDDMELEQKMSELDPA
                     TLNSDERPGNVSSLTCPDCSGPLYEIREGELSRYRCRVGHAYGSAEDVLEKNSDKLED
                     TLYFALNKLQENAAMARRLAARAHEAGQEMAAARFEERERESRQHAETLRRVLLGSPL
                     PKEQTSATSDTVGAP"
     gene            complement(9762..10703)
                     /locus_tag="VN178_10130"
     CDS             complement(9762..10703)
                     /locus_tag="VN178_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS81369.1"
                     /translation="MSIFPTKVLLATDGSKEADLAARSAAELAGKTGSELHVVHVFGI
                     SPWYAAYPEATRFDGVALEDQVLEEDVQRISQQRARELLDAEAEKLRSVGGTLAQTHL
                     VEGGVPQEIVGLAETIGAGLIVVGSRGRGGLRRALMGSVSDSVVRHAHCPVLVVRDGE
                     SPRDFLPGRILLAVDGSEEASTAAQTTAELAERTDSELHVVHVGEVEPVFHPERHGYR
                     ARYEELQEEARRLLEGQVGEIEAAGVTVSRAYLRMGRPDEEIVGLAEEIGADLIATGS
                     RGVGGMRRALMGSASDSVVRHAHCPVLVVRTKEEQAS"
     gene            complement(10846..11709)
                     /locus_tag="VN178_10135"
     CDS             complement(10846..11709)
                     /locus_tag="VN178_10135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HWS81370.1"
                     /translation="MRRVVLVLATMALAVLLAGGVAQAVINGEPDRGPDAHRFVGALV
                     TVPPSGEFKGQRIPVCSGTLISPKVFLTAGHCTDLLTREDLPTYVSFDPTYKPKASKT
                     IKATPHTHPTFCFPNSENKGECTPSGRPEIVGSLARDIRYDVGVAVLDEPVRMASYGA
                     LPVAGLVDTLEVGQRLTAVGYGTTGYEIGGGSSRPQLVYPRDRNSATVRLLDTSDALS
                     GMFVKTTGVNFVNGKGEGSCKGDSGGPLFVPDQQTIVGVTSFGYSVCRGSVYYQRTDL
                     PVVLKWVRSFP"
     gene            complement(12014..13138)
                     /locus_tag="VN178_10140"
     CDS             complement(12014..13138)
                     /locus_tag="VN178_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWS81371.1"
                     /translation="MKRLFELICITSIVVLSALFVIGADRVVVVPCGQDLDAIVNADD
                     PTLGTRFQLEGPCTYPVDTMVVLNEGDELAGPAGSFIERYPAFDPEPTVTIVGSEGLR
                     NVIRAKGTVRVEWVKIVGGTGQYAGGSPLAGTGSGLAMGKASNTSSLYAVHITGTDAA
                     GITNAHGTFDRIELDDTTQDPNFLGFTGSGLKAINEVEVRNSYIHDNQGNGLWCDVYC
                     HDSESHPNGFWVHENLVVNNGRAGIRFEEVGDVADSGEALIENNEVHGNSTGAVRGGV
                     SIHDAQNALVRNNVFGAAVIAGVAYLPDSEGVAIRATDSGRPDRPDLWNVDIAGNVLN
                     GEVIKGCESPVEIVYCSEVALPPLPTYTTPPQTTIPLYGP"
     gene            complement(13462..14028)
                     /locus_tag="VN178_10145"
     CDS             complement(13462..14028)
                     /locus_tag="VN178_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81372.1"
                     /translation="MAKQNYEEKYTHPELRESVKEEIKASDKGGKEGQWSARKSQLLT
                     QEYEKRGGYKGEKGQSQKDLEEWTEEEWQTKEGEADARQDSGETKRYLPKKAWENMSE
                     EEKEVTEQKKREGSKKGQQYVSNTEEAKEARKNAKEVPISGYDDLSVGEVEKKIEGLS
                     KDEIRAVRSYEKNNKNRKTLLEKIDGKL"
     gene            complement(14278..15552)
                     /locus_tag="VN178_10150"
     CDS             complement(14278..15552)
                     /locus_tag="VN178_10150"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWS81373.1"
                     /translation="MNDTGSTLTHLEGALSGESYPADELIGLDPVDERPLLARYDIEK
                     AARTLRRKSLAERRTGGLWRWAELLPVRDKSNVVYLGEGDTPLLAQPRLGEALGVPRL
                     STKAEGLNPTGSFKARGMAVAVSRAVELGVTSFIAPSAGNAAGALAAYGAAAGAQVMV
                     LTPEDATPVNQQEVLAMGARLVLVRGLISDCGKISTALAPKIGAFDVSTLKEPYRVEG
                     KKTMGFELAEQRGWRLPDLIVYPTGGGTGLVGMWKAFQELETMGLIGPDRPRMVSVQS
                     EGCAPIVRAFESGDRFAEPWQDAKTRASGIRVPSAVGDFLILDSLRESGGTAVAVSED
                     SISEMQMFAGRMGAGYVSPESAAALAAVPLLRDRGEIGRHDDIVVFDCGIGQKYPPPR
                     NLPELPIVDPAGLDIDELAHRLRQSDAQAYLN"
     gene            15821..16966
                     /locus_tag="VN178_10155"
     CDS             15821..16966
                     /locus_tag="VN178_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS81374.1"
                     /translation="MYEQINPFVYGRPVYDEEALADRATEKRRLISDANSGQPVILYA
                     PRRYGKTSLARVVSGRLLEDHLVPSVYADFWGAASISDVVEVLGRAYAVASVRQRARR
                     FFADLLRSVGFSVEFAGTAVSVSYEGKGRNEQQRAALMRLLDVPQQMAVRSPSQRRLF
                     MVLDEFGEVFNVPGEPYALMRNAFQASPNVSFIFMGSKRSLMDALFSERHHPFYNFGR
                     RMSLGRLPFEELGAFIEGRFASFGKRVTPEAVDLLLSLTDGHPHRSQQLAYHAFELAS
                     GEADEESILSAKEAAFDETADEFRVLLDRMSTAARAVYLAVCKDPTTEMTSRVYLQRH
                     GIKSTGSLRSAIRTLTDAGDLEQTGAKRPVPTDPLLAAWVRERMNRG"
     gene            complement(16984..17610)
                     /locus_tag="VN178_10160"
     CDS             complement(16984..17610)
                     /locus_tag="VN178_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWS81375.1"
                     /translation="MAHIEGFGGRGGFGRRPALAVIDMTLGFTDPESPLACDLEGPIS
                     EIQKLLRAARRAEIPVVFTTVAYRESDKLTAAAFIDKVPALLTLQAGTRWAEIDPRIA
                     SEETEPVLNKLFASGFFGTGLSSLLTAAGVDTLIITGASTSGCVRATAVDALQYGFRP
                     IVPSEAVGDRNPDAHKANLYDIDAKYGDVVSVEETLDYLEAMRAARVG"
     gene            complement(17615..19510)
                     /locus_tag="VN178_10165"
     CDS             complement(17615..19510)
                     /locus_tag="VN178_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWS81376.1"
                     /translation="MVLHEQAEPDFVRDHDIDHVTLDIIENALKNIRHEMDRVLVTTA
                     VSPVIREQADEFPLIADRQGRMVVGQFGSPVDTVLENSPYTVEDLKDGDVIALNDPYM
                     MEGSTSHLPDILLVRPIFYEGDHIGYALQWGNLMDVGGSTAGSIPIDARSIYEEGVRL
                     PPVKLYDGGVLNEEVLRFFCHNSRTPRETRADVMAIAAGTAAGAQRVKDICDRFGKDT
                     YLEACDALLARTRTSIINLIRQLIPEGERFEFEDYTDDDGLGNGPIKLKLAMWREGDS
                     LHLDWKGTDAQVPGPVNFLLNKEMFQMFAGVFLISAVDPTILFNDGYADIIKVNIPDG
                     TVLRPREPAPLSNRLVVMARQFDVLGAVYAKAIGFNVSGSYGTSPNFVYSGVKEDGES
                     FQTMEILYGGIPAIPGKDGLDGHSWWPEFLAVPAEYMETYYPMIVEEYSARKDSCGAG
                     QFRGGCGIKKVYRFLADGRITYQDDRHHTYPYGVDGGKPGAASKKTLVRADGEEVDMP
                     AKVRDVPVFEGDRLIFETAGAGGVGDPLEREPEAIAKDVRWNLISPEVAESVYGVVVN
                     EDGSIDAGATESKREEIRNTRGESPGFDHGNLPPLDEQRRMIAETRRKFNEWLSGELG
                     KARGNGG"
     gene            complement(19514..21565)
                     /locus_tag="VN178_10170"
     CDS             complement(19514..21565)
                     /locus_tag="VN178_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWS81377.1"
                     /translation="MYNVAVDVGGTFTDVLVFDEETGGLTEGKVLSTPDDPSKGVVEG
                     IEAVCEKVGISFPDLHLFFHGTTVVTNMILTNTGSRVGLLTTKGHEQILHLARAWTPG
                     PLYGWMALQKPDPPADPTDTVGIAERISSGGEVLTALDENEVREAVKRLVDRGVESLA
                     IGFLNSYVNPSHERRARDIVRGAYPDLSVSISADIGQEYGEYERTLTTVVNTAVQPRT
                     MLYMRNFEKSIDEKRFGGALSIVRSDGGAMSTGAVAESPIQIALSGPSGGVTGSAYLA
                     REIGVPDVLTFDMGGTSTDVSLCLNGETPVRRDIQLGYYQFKSPAVDVHSVGAGGGSI
                     AFVSASGALQVGPDSAGADPGPAAYGRGGVAPTVTDANVVLHRIPPGVALGGTLEMDE
                     EAAREAVGTITERLGMGVEEAAEAIIEIVNENMHAALRVVSVERGHDPRNFGLVAFGG
                     AGPMHANALGRLIHSRSVIVPPTPGVMSAFGFLSSDIQNEFPETYLRVAEETRAQDLK
                     STIEGLIAQADEWLEGEGVEEDKKLFDLYADCRYYLQNIQIPCRFELEELDDGSSAFL
                     RGRFEEAHRQRYNFDLPDSPLEIATVRVVGRGTIKGVSLLESEDGAGQDASGAITRRE
                     RVYFDGEWNETPIYDRAGLRPGNAVAGPAIVVQDDTTTVIEPGYRGAVDSFGNILIEE
                     A"
     gene            complement(21777..22658)
                     /locus_tag="VN178_10175"
     CDS             complement(21777..22658)
                     /locus_tag="VN178_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HWS81378.1"
                     /translation="MRIEVVDVGPRDGLQNEDKILSPEVRAELCEKLAAAGVPRVEAA
                     SFVNPKRVPQMAGAEEVMSAIERSPGTIYAGLVLNEKGYERAIQSGVDEVRYAFPVTE
                     TFARRNQNTTVADATQLAARLVERARLDGVRVSITLSAAFGDPFEGRVDPDHVLRIAS
                     SVAASGPDEIVLADTIGVGVPNQVRNLVEEVATHGITVACHFHDTRNTGIANAAAAVE
                     SGATVLDASVAGTGGCPFAPRATGNIATEDLVYLLHNMGYTTGIDLDALIEVATWLAR
                     QLDKELPGQVYKAGNFV"
     gene            complement(22658..23872)
                     /locus_tag="VN178_10180"
     CDS             complement(22658..23872)
                     /locus_tag="VN178_10180"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWS81379.1"
                     /translation="MAEGSNGIRPLEGVRVVEMGSLLAGPFCGQLLADFGAEVIKLEP
                     PGKGDPMRVWGRHRKEGRTLWWPIIARNKKSVTINLREDEGQDLARRLISEADIVVEN
                     FRPGTLEKWGLGYEDLSGNNPGLVMVRVSGYGQTGPYKDQAGFGSIGEAMGGIRHVTG
                     YPDRPPPRVGISLGDSLAATFGALGAVTALYNRETRGGKGQVVDVGIYEAVLALMEST
                     IPEYALAGHVRGRTGAILPFVAPSNTYPTGDGDYVVIGANADTVFGRFAKALGHPEWA
                     EGEEYATHHARGENQEELDALISDWTGQRSAEEVLETMKEAGVPAGKLFTAEDMLSDE
                     QYAARQNIVTVEDPEIGPFPMQNVVPRLSETPGKVRWTGPKLGQHNDEVFEKVLGIAG
                     EDLDRLRERGIV"
     gene            complement(23865..24554)
                     /locus_tag="VN178_10185"
     CDS             complement(23865..24554)
                     /locus_tag="VN178_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWS81380.1"
                     /translation="MGYAVRGPGRGSRSVGIVERLRALILTGEYGPDERLIEEQLAER
                     LGVSRTPVRQALTMLEAEGLVEIAPNRGATVCSFSIEDVWDIYDLRAVLEGHAARRAA
                     GRIEKEELERLEELAGEMEGLPGRFDDHEEEIRTLVALNQEFHGTIIQASRNRRLEHL
                     INRTVELPLMFKAFFWYTPHERTISNHYHRQILESLENGDADRVEIIMREHVYDGRDF
                     VIGALEEDLNG"
     gene            complement(24650..25447)
                     /gene="map"
                     /locus_tag="VN178_10190"
     CDS             complement(24650..25447)
                     /gene="map"
                     /locus_tag="VN178_10190"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWS81381.1"
                     /translation="MVVLRSKNEIRGLRRAGDLVARALLLLHEHVRAGVKLRELDRVA
                     EDFIRSEGGVPTYKGYQPSPSVPPFPGTICTAVNEEIVHGIPGPRRLRDGDIVGIDVG
                     ATLGGWVGDTCYTYPIGNISPRARSLLDVARECLEAGIAEVGPGKHFGDVGAAIQELA
                     EHHGYGVVRDLGGHGVGHKLHEEPHINHFGRRGRGMRFREGMTFTIEPMINEGTPEIR
                     YLADGWTVVTVDKRLSAQFEHTIAVTSKGCEILTPWHLLMRETADLA"
     gene            complement(25551..27494)
                     /locus_tag="VN178_10195"
     CDS             complement(25551..27494)
                     /locus_tag="VN178_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWS81382.1"
                     /translation="MSFESLLVANRGEIAVRIIRTCRELGIKSIAVYSEADEHAMHRR
                     LADESYLLGPAPAPESYLNVGRLVEVIERSGAEAVHPGYGFLSESAQFARAVGSAGAT
                     WVGPSPEAMDAVGFKVRAKELARRAGVPTIPGYDGEDQSEERLAKEADLIGFPVLVKA
                     SAGGGGRGMRSVTLQKDLVEAVRGARREAEASFGDGSVFLEKLVEDPRHVEVQVIGDQ
                     RGNILHLFERECSIQRRHQKVIEEAPSPALDPDLRDSICTAAVRYAREAGYHNAGTVE
                     FLLDDDHYYFLEMNARLQVEHPVTEFVTGLDLVHLQLSVAANVPLPLTQDEVTLRGSA
                     IEARIYAEDEQGMPSGGLLETFSLPEGAGVRNDAGYEAGDEVPINYDAMLAKLIVSAP
                     DRPSAVRRLRRALTDYEILGPSSNLPLLRRIATHPAFEAGETTIRLLETYDLTEPQPE
                     KPLPREAMLLASAAELYSIPSSSDPFAAGPWRLLGATTLDYDEHRVEAERAAPGTFRI
                     TAGGDESIVEILYHGDTRIRALIDEEPVEAGIILGPEEVLISLRGETHTLRKPLPPSV
                     DETGPGADAAASLTAPMPGTVVKVLVEEGQEVEEGQLLLVLEAMKMEQPVSAPHAGVV
                     HSLPFEEGSLVPGGAVLAEVRET"
     gene            complement(27491..28276)
                     /locus_tag="VN178_10200"
     CDS             complement(27491..28276)
                     /locus_tag="VN178_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWS81383.1"
                     /translation="MPDLLKVSRSGPLAAVTINRPDSHNALNADLIEELTRRFEEISD
                     DDRVRVVVLAGEGRSFCAGADIGYMRETAGLSYEENLEDARRLAMMYWTIDECPKPVV
                     AKVQGAAMGGGAGLLAVADVAVADSEARFAFSEIRLGIAPATISPFVVRKIGVSHARS
                     LFLTGERFYAKRAWEIGLVHRLVSSDGLDEAVDEKARELLEGGPVAQATLKGLLRRLE
                     TTEPMEAPGLTARVISELRTGEEGQEGLAAFLEKRDPSWRRAR"
     gene            complement(28304..28732)
                     /locus_tag="VN178_10205"
     CDS             complement(28304..28732)
                     /locus_tag="VN178_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWS81384.1"
                     /translation="MRAAVIIVENGRVALIERVRDRHTYFKFPGGRVESGESPQQAAV
                     REAHEELGVGVELGDLISVAHRDGREQRYYLASIVGGTFGTGRGTEMITSGTTAKGTY
                     RPVWVDLTSLTELDVRPRALAEALSSWTGEGEIGWLGEFE"
     gene            complement(28735..30342)
                     /locus_tag="VN178_10210"
     CDS             complement(28735..30342)
                     /locus_tag="VN178_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWS81385.1"
                     /translation="MSKLGTIVDTKSGAFEKNAEAFSGLVDGLRMHSAEARRGGGEKA
                     QRRHRERGKLPVRERIERLLDPGTAFLELSPLAAFGMYDGNVPAAGIVTGVGRVSGRE
                     VMVVANDATVKGGTYYPMTVKKHLRAQEVAEQNYLPCIYLVDSGGAFLPLQDEVFPDR
                     EHFGRIFYNQAQMSAKGIPQVAAVMGSCTAGGAYVPAMSDEVVIVKGTGTIFLGGPPL
                     VKAATGEEVSAEELGGAGVHTRLSGVADHFAENDEHALAIVRSIIENLNREKHVPWTV
                     EEPEEPLYDPEELYGIVPSDYRQGYDVREVIARTVDGSHLHEFKPLYGETLVCGFASI
                     MGHPVGILANNGILFSESALKGAHFIELCCSRGIPLVFLQNITGFMVGREYEAGGIAK
                     DGAKMVMAVANASVPKFTVIVGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGQQA
                     ANVLLTVQEESLGREDKEMSDEEREDFKAPILEKYEDEGNPYHSTARLWDDGIIDPVD
                     TRMVLALGLSAAANAPAEETRYGIFRM"
     gene            complement(30534..33914)
                     /locus_tag="VN178_10215"
     CDS             complement(30534..33914)
                     /locus_tag="VN178_10215"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HWS81386.1"
                     /translation="MLQKVLVANRGEIAIRAFRAAYELGIRTVAVYTPDDRASLHRQK
                     ADEAYEIGEAGHPVRAYLDVDTLVETALQVEADAIYPGYGFLSESADLARACEEAGLV
                     FVGPPSDALELTGNKLRARRAAEEAGVPVLKASGSVADPGEAASVAESIGYPVFVKAA
                     GGGGGRGLRLVERPEDLKGSVRNAAREAEAAFGDPTVFLEQALIRPRHIEVQVLADAA
                     GEVVHLFERDCSVQRRHQKVLEIAPAPNLTLRLRERLCEEAVRFGRAVGYLGAGTVEF
                     LVGEDGHHVFIEMNPRIQVEHTVTEETTDVDLVHAQLRIAGGETLRDLDLAQDRIRQR
                     GVALQCRVTTEDPAQGFRPDTGRISVYRSPGGAGIRLDGGNTYAGTEITPYFDSLLVK
                     VTAHGADLPSAAVRARRALAEFRVRGVATNMAFLRAVLSDPDFLAGRATTSFIDERPH
                     LTAVSAGADRASRLLSLLADVTVNRPHGPPPRVPDPRTKLPPLAQGDPPPGSRQRLVD
                     LGPEGLARWLRETDTLQVTDTTMRDAHQSLFATRMRTFDMLAAAPHVARTMPQLFSAE
                     VWGGATFDVALRFLHEDPWERLARLRERLPNVCLQMLLRGQNAVGYTRYPPDVVRSFV
                     DEAHESGIDIFRIFDANNDVDHIRPAIEATLEAGAVAEGAVCYTGDLSDPNEDLYTLD
                     YYLRLAEELVDAGSHILCIKDMAGLVRAPAARKLISALKSEFDLPVHLHTHDTSGGQL
                     GTYLAAVEAGVDAIDGAAAPLSGMTSQPSLAAIVAASDHTDRATGLSLEALGDLEPYW
                     EAVRTLYAPFEAGLRSPTGTIYRHEIPGGQLSNLRQQALSMGLAERFEEVEALYARCD
                     TLLGRLVKVTPTSKVVGDLALYLLSANIPLADFESDPASHDLPDSVIGFLRGELGVPP
                     GGWPEPFRSRALAGRDESPIDDALSAEDRTALGGENRRPALNRLMLPGPAKEQAAVRE
                     RYGDVSVVPTRAFLYGLEPGEELAVDLEPGVRLYVRLEAITEADGRGIRTLLVTLNGQ
                     PRPIDAQDRWLQPEVPVREKAVPGEDGHVAAPMTGVVTLTVDEGEEIVGGQQIGSIEA
                     MKMESAIRAPAGGTVERLAVASGTNVEAGDLLIVLEVG"
     gene            complement(33939..35126)
                     /locus_tag="VN178_10220"
     CDS             complement(33939..35126)
                     /locus_tag="VN178_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS81387.1"
                     /translation="MNFDPTHEQRRWRDLAREFAQGEIRPRAAELDREQRFPYDIVAE
                     MASLGLMGLTLPEGYGGSGGDFVAYTLALEEISRADTSVGITMEAHISLGCAPIAAFG
                     TQEQKEKFLSRCATGENLWAFGLTEEGAGTDAGGTRTKAELDGVEWVVNGSKKWITNS
                     GTNITGGVTIIARTGTRENGTPELTAILIPQDTPGYTRGPSYEKLGWRASDQHPLFFE
                     DCRVPEENLLGERGKGFNQFLATLDAGRVAVAALSVGLAQACFDEALARTQQREQFGR
                     SISEFQAVQFKLADMAMEIELARNMVLKAAWLKDQGRPFGREASMSKVFASETAKRAA
                     DQAVQIWGGEGFMETSAVARYWRQVKINEIGEGTSEINRQIIARSLLAESAEGAQERL
                     VAQ"
     gene            35292..35555
                     /locus_tag="VN178_10225"
     CDS             35292..35555
                     /locus_tag="VN178_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWS81388.1"
                     /translation="MLGALIFWIIVGLVAGALAKFIMPGDDPGGFIITIILGIAGAIV
                     GGFLASLIGIGGGGFIWTTLIATVGAIILLAIYRAFAGSRAAG"
     gene            complement(35636..37303)
                     /locus_tag="VN178_10230"
     CDS             complement(35636..37303)
                     /locus_tag="VN178_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWS81389.1"
                     /translation="MRPEDIDCLTVGGLLDLVAGRRPEDEALVYADRNLRYTYTEFKA
                     VVEKCARSLMALGIDKGDHVAVWGQNVPEWVILQFATGKVGAVLVTINPAYKSHELKY
                     VLEQSDAAVLFLTEGAGDANFLQILQGAVPELGEAAEGELSIEELPFLKHVVLMGGDS
                     PEGLPVIGFDEFMEKAEEVSDEELRERQSSLDAEDVINMQYTSGTTGFPKGVQLTHAN
                     IVKNAFYIGECMKLGPGDRVCIPVPFFHCFGCVLGTLNTVTHEGTMVPVESFDAERVL
                     QAVDQERCTALLGVPTMFIAELEHPDFEQYDTGSLRTGIMAGSPCPMEVMKKVVDVMG
                     ASEITIAYGQTESSPVITQTRTDDPLELRVSSVGRKLPDVDVRIVSVETDEDAGPEEQ
                     GELWTRGYHVMRGYYKMDEKTSEVIDDEGWLHTGDLAVMDEDGYVRITGRAKDMIIRG
                     GENVYPREIEEFLYTHPEVSDVQVYGVPDQKYGEKVAAAVKPREGSGLTAEDVQNYCR
                     ENIAYYKVPEYVDFVEEYPMTASGKIQKYKLRQTAVERYGLQDTTIP"
     gene            complement(37547..38485)
                     /locus_tag="VN178_10235"
     CDS             complement(37547..38485)
                     /locus_tag="VN178_10235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HWS81390.1"
                     /translation="MSNDVTTERAALIVNTRSRSGERTFFESLDRLQQLGVPLGVTYA
                     IRDPARLPETVREVLSDGSGYKFLILGGGDGSVSSVVDFLANHDTLLGLLPLGTANDF
                     ARTLGIPQDIEGACKTIAEGKIVDIDLGLAGDNYYVNVASVGLGVEATRALSPWLKRS
                     TGPLAYPAAAIKAFLRHEPFSARLTFPDGDHEPVEYDRLLQVAVGNGRFYGGGMVVAP
                     ESGIDDKLLDIYAIDLGRHRDLIGVARYLKSGDFIKMEGVHHFRTSRVQIETDPDLAV
                     NIDGEVVAMTPQDFSVAQNALHVLVPQESDAARHDE"
     gene            complement(38680..>39199)
                     /locus_tag="VN178_10240"
     CDS             complement(38680..>39199)
                     /locus_tag="VN178_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS81391.1"
                     /translation="ELAGGGAVMDHTVHLADLLRWMLDVEVKGVYAEVDSFFGAGGAD
                     DAAILTLDLEGGSYADGAFATIDPSWSRGDGYPTWGDVTLRISGTSGVLDVDPFAQPL
                     RMFDHETRAPSWSYMGEDMDALMLADFLRGVAEGVPAGASGLDGLRTLEVVLAAYRSA
                     EDHEPKRVEQTH"
BASE COUNT         6619 a        12432 c        12355 g         7793 t
ORIGIN      
        1 gtatagacca gatccgttcc caggctagcc ggcgcaggac gcaagaaaga gtcgaagcct
       61 cggccccacg ctcccaaccg agcgaggcct tcgcggaaac ctggaggaac tcgcagcgcg
      121 accaggtcag ggaggccctc aacactctcc cgtccgagca gctcaagata ctggagcttg
      181 cgtacttttc cgggtacacg cacgtggaga tatccgacct tctcgacctg cccctcggga
      241 cggtcaaggg caggatgcgt ttgggattga agaagatcag ggactacttc gagtcaagaa
      301 gcgcggcggt gccgagatga gtgagatgaa caacgaacgc ttcgaagatc tcaaagacgc
      361 ctacgtcctc ggtgcgttgc ccgaggagga gaggctgagt ttcgaggact atctcgccac
      421 ccaccccgaa aggcaggccg agatagatga gcttggcgcc gtggccggcc tgctcgcgtt
      481 ctccccgcaa gaacaagaat cctcgccaga gctgcgaagc aggattatgg aggtggtgga
      541 ggccgaggcg gagccccgcc gcgacacgcg ccggtccgtg tctgcaagta ttggggacta
      601 tctcagcgtc cgtagcctcg ccctcggcgc cgcggccctg ctcgtgatcg ggttgctatc
      661 gtggaacgtg ctgttgcagg gtcaggtatc ggacctacag ggccaggtcc ggaacgcgca
      721 aggccaggta gaggatcttc aagcccaggt agaggatgca caggcccaac aaagccaaac
      781 gatcaagctc gagggatcgt gggcagaaca gggggccacc gccgaagtgg cctcaatgga
      841 agagaacgag atcgtcctcg tggcaaggaa tatgccttct gtacccgacg acaggacatg
      901 ccagatctgg gtcataaagg acgacgtgcc caaacccagc ggtctcttcc aacccgacgg
      961 taacgggacg gcgacgccga tcacgaactc cataacgaat gccgacgtga tagcggtgac
     1021 cgtcgagcct gccgggggct ccgagcagcc gacgagcgac cccgtactcc tggcggagct
     1081 ctagggcggg tctgagcccg ggcgggtctc ctgagatccg cccctacagg gtaggggttt
     1141 cggggttcat gatttgggct gcgggcgatg ggtgccactt ggttatccga tcattctgga
     1201 aggcgaggtc gttcacgttc ctggggctcg ttatctgccc gatgcatcat tgccacagag
     1261 cgtttatttt ctgcaggcgc gggtagtaaa tatccaggga agcagagggc aagccaggtc
     1321 aaaggagagc atatgtccgt agccagagta accgaactga gcgccgtctc cgagacgggt
     1381 ttcgaggacg ccatcaggca aggcgtggcc cgcgctacct ccaccctgcg gggtgtcgag
     1441 ggcgcctgga tcaaggacat gaacgtcctg atcgaggacg gtaacgttac gggttacaag
     1501 gtgaacatgg aagtcacctt cgtcctcgaa gattcataga gacacaagcc acgcttaggg
     1561 ttttgtgccc cggctctcgg gccggggttt tcttgcgggc ggttcgtgaa ccgcccctac
     1621 atgccgcggt atcggacgtc aggcgagacg tgtgtatttc ggtaacgtgt ggggccggcg
     1681 gccgattgcg gcggtgggaa gcggaacaca gcgggcgctc gagcggcgtt gtataccggg
     1741 ggcgtgatac gactcggata ctcgacacag aacctgacga tccaggcgag cacgaaccgc
     1801 acgctgcggc tcgccagcct ggtagacgcg gagagagtca agggactcgt cagggagaac
     1861 atcgccgatc taaaacggat cctacggtgg aatgcggagc atggggtcgg gctcttcaga
     1921 atggggcaga acttggtccc tttcgcctct catcctgcct ttccttacga ctggaaggcc
     1981 gagcacggtg cggcgctgag cgaagcagga gagctggcac gagacctggg cctccgtctc
     2041 tcgatgcatc ccggccagta cataaacccc ggaagcccga agcccgaagt ggtcgaacgc
     2101 agcctgaaag agttgcgtta cgtgacgagg gtgttcgatc tccttggcag ccccgacggg
     2161 gtggccgtgt tacacctggg tggtgcctac gcggacaaac aaggtagcgc cgcgcgcttt
     2221 ataggggtga tgcggtccga ggcgcgggtc ttgcgctatc tcgcactgga gaatgacgag
     2281 cgggtttgga cagtcgccga ggtcacccgg acggccgatg cccttggcat cccggcgatc
     2341 acggacgcct ttcaccacga actcaatcct ggcgacctga cgctggagga ggcgctcgac
     2401 ctctcgctgc ccacgtggac aacccggggc gtgcgtccca agttacacct ctccagtcag
     2461 gaccctgcca aacgggccgg tgcgcacgcg tactcggtgg acagcaggga ctggcaagtg
     2521 ctgctcggcg ccattggcgg taggaaggcc gacgtgatgg tcgaggccaa aggcaaagag
     2581 cacgcccttg cgccgctggg tgtggagatc gtataacatc ggggttagag atccctgagg
     2641 aagagagcag catgcacgca aaccctgacc ttcgagagat aagagacatt ctgcaacgct
     2701 cgggcagagt ggctgtcgtc gggctttcgg acaggcctta ccgcaccagc caccgtatcg
     2761 ccggcgcact gcagggcttc ggattcaaga tcttccccgt caacccgaat ctccgcggtc
     2821 ctgtactcgg cgaagagcct tacgagtcgg tccaggacat ccccaccgcg gtggacattg
     2881 tcgacgtgtt ccggcgtagc gggaaggtca tgccggtggc cgaagatgcc gtggccgccg
     2941 gcgcgaaggt actgtggatg caatctggtg ttatcaacga agaggcggcc gcctacgccg
     3001 aggagcacgg ccttaccgtc gttatggacc gctgcataaa ggtagactac gcgacgctgg
     3061 tcggctccgc gacttctcca agaacgtagg tttcaggctg caggtttcag gtttggaccg
     3121 gtaggcgatg ggctacttcg gtggttcgcc gctggttatg ttcttctacg ggacgctcaa
     3181 gcgtggtgag cgtaaccatg agcatttctg cggtggggct ttacgcgtcg aagagggggc
     3241 ggtgcggggc gaactgttcg atgttcacct gggttatcct gcgctggtcg ttccggaaga
     3301 gtccatctgc gctttcggga ccggcgatta tagaagggac gcggaggagc agcaacggct
     3361 tggccgtggg caggcgccgt tgctggaggg tccacgtgtc ttcggtgaga tcttcgcttt
     3421 cgacgatccg gaatcgcgtc taccggacct ggatcgtctg gaaggctttg accccgccga
     3481 tgcctctagc cactaccggc gggtcttgct gcccgtcgag acggacgagg gctcgggcct
     3541 tctggcatgg gcctacgtgg tcgagcaatc atctggaagc cacctgccgg gaggcaggtg
     3601 gccgccctga gggcggcgaa ccgcccctac aggcaaaccg ctctagctgc gtgtgtccgg
     3661 agcgtcgtct tcttcgatga tctcctcgcg tatgacgcgg cgacggatct tcgagcgtcc
     3721 gccttcccgg aaggtctcgg tctcctcggt ctggcgcgtg gtaggcgtct catcggtgaa
     3781 gggatctgtg ctgcccatgg acgatcgacc ttccgaggcc cctctcatgt acccggttgt
     3841 gtctctgggg tcatcgcctc tctcggtagg ctccgcacgt tccccgtact cggggtctgc
     3901 ggaagtacgg tcgtagtctg tagaatcgac ttccgcttcg aagtcctgcg gctcgcgcgt
     3961 tctgtcagcg taatcgccgc cacccgagta tgctccgtag ccaccgcggg ggaaggttcc
     4021 gacggattcg aggccgaagt ggctgcggat acggtcttcg tactcggcgg tgatgtcctc
     4081 gtcgtcgtcg aagctcggag catccttcac gttggccttc gactctgcga cttcgatcgt
     4141 tctttcacct tcgttcaccc ggacgatatc catcgggatc agggtcgatt tgaggccaaa
     4201 gaatcccatc ttgacaccga tgtattcttc gcggtccgtc tcgtccacga agaggtcgtc
     4261 gacttttccg atcctctccc cctggttgtc gtagaccttg tagtcctcgt acttctcctc
     4321 gagttccctc aacctatctt cgctcgccac gatctcctcc tggataagcc tgcggctgtt
     4381 agtgatctgg gtacccgagt cagggttgcc ggtaaacatg gatgctcccg cagaacgatg
     4441 gggggaagcg gcttgctaaa ctaccgtgaa tggtgatcct cgagggaggt gggagcgtgg
     4501 tatccagaga atctgggggc gcaaggattc tctctgtaga cgttggttcc tcgtcggtga
     4561 gggccgagct ctacgacgac gctggcaacg gcgtagcggg gaccgaggtc agcctcgact
     4621 acgagttcga gtacacgccg gaaggcggcg cggtgaaaga cggcgacgag ctactcgacc
     4681 tcgtagctcg cgccgtagac ggcacgctct ccaaggccgg tggaacggag atctccggcg
     4741 tcgccatgag cactttctgg cactcggttc tcggcgtgga cagggacggg cgtcccacga
     4801 ccccgatcct gacctgggcc gaccgccgcg cggcagagtg cgtgccggat ttgcgcgagc
     4861 gcctggacga gacggccaac caccgtcgga cgggttgcgt gccccactca agctactggc
     4921 ccgccaagtt gctgtggtcg ggctgcgcca tgcccgagac tttcgaaaag acggaacgct
     4981 gggtttctcc agccgactac ttctacgccc gtttcttcgg ggagccctat caggtaggaa
     5041 cgtcgatggc ctcgggaact ggcctcttcg accagaaccg gcggaagtgg gaccgcaaga
     5101 cgcttaaagt cctgcccgcc gaagaaggcc agctctccgc catctcggat gagccccgta
     5161 gtggtctggc cggagagtgg gcagagcggt ggccggccct gcgagatacg ccctggctcc
     5221 ccgcggcggg cgacggcgcc tgctcgaacg tcgggagcgg atgcacgggt agtgaccggc
     5281 tggcgctcat ggtaggcacg agcggcgcga tgcgggtgct ttggaaagcg gagtccgtcg
     5341 agatacccga cggcccctgg tgctacaggg cagacgcgca acgatacgtc atgggcggcg
     5401 cgctctccga tggcggaaac ctgatcgaat ggctgcgcaa cacgctacgc ctccctgacc
     5461 cggagaagac cgagagactg ctgtccgaga tggagccgga ctcccacggc ctcacgttcc
     5521 tgccgctgct cgccggagag cgtgggccgg gatgggccga cagggcaaac gggaccgtgt
     5581 ccgggctctc gatgagcacc aagccggtcg agatcctgcg cgccgccatg gaggccgtgg
     5641 ccttcaggtt cgcgatcatc gcagagatgt tggagcgcgc ttctcccggg gagaaggagg
     5701 tcatcgccag cggcgggggc ctcatccact cgccgacgtg gaccaggatc atggccgaca
     5761 ccctgggcag gccggtgacg ctctccggtg taaaggaggc ctccagcagg ggagcggccc
     5821 tgatcgccct ggaagcactg ggaggaccgg agatcgaagc cgccgacgtg ccgctcggcg
     5881 agaccttcga acccgacccg gcgagacatg aggtctacat gaaagcgctc ggacgccaac
     5941 gccggctcta cgacgccgtg cttggcggcg cgtagcgccg ccagctagtc agcacgctac
     6001 ggccttcggc ctccgcttgt cagcatatca gcacgctcaa gcctccggct ttcgcttgtc
     6061 agccaggact tgcacatgca aaagctgacc agctgaagtg ctgactggct gtcaaggcgg
     6121 cggagccgcc gtgctgacgg gcgcgtcagc gctcgtctat cggcacgtag tctacgtcgc
     6181 gggggccggt atagatctgg cgcggacggg ctatcctgag ctcggggtcg tcgatcatct
     6241 ccatccactg ggccatccaa cccgaggtgc ggggtatggc gaacatgacg gtgaaagcat
     6301 ccgtcggtat cccgagggct tcgtagatga ggccggaata gaagtcgacg tttggataga
     6361 gcttgcggct cgtgaagtag tcgtcgtcga gcgcgtgctt ttcgagctcg atggcgatgt
     6421 cgagcagcgg gctctccttg ttcgtggcct cgagcacctc gtcgacgtgc ccgcggatga
     6481 tcctggcccg cggatcgtag ttcttgtaca ccctgtgccc gaagcccatg agtcgctcgt
     6541 cgccctgctt gacgccttgg aggaaggcgg ggatgttctc gatgcttccg acgcgctgga
     6601 gcatcctgag aaccgccacg ttggccccgc cgtgcaacgg accgtagagt ccggcgcagc
     6661 ccgcggcgat ggccgagaag gggtcgaccc tcgaagaccc gaccaccctt accgcagcgg
     6721 cggaacagtt ctgctcgtgg tcggcgtgca gtatgaagag cacgtcgagc gccttctcga
     6781 ggcgcggatc tggctcgtac ttgggctcgg ccatcttgaa gagcatggac aggaagttgc
     6841 ccgcgtagga gaggtcgttg tcgggataga cgtagggcaa tccgaggctg tggcggtagg
     6901 cgaacgcggc gagggtcggc accttggcga tcagacgcac cgccgcaacg tggcgctggt
     6961 agtcattgtc tatctctttg gcctcggggt agaacgtcga gagggcgccg acggaagcct
     7021 gcagcatccc catcgggttc gcgtcgtgac ggaagccgtt catgaagttc ttgatgttct
     7081 cgtggacgta ggtgtgatgg gtgatctcgt aggtccactg gttcagctcg tcctcggaag
     7141 ggagccaacc gtagatgagc agataggcca cctcgaggta ggaagagttc tcgcacaact
     7201 gctcgatggg gatgccgcgg tgctcgagga tccccttctc cccgtcgatg taggtgatgg
     7261 cgctcctgca actcgccgtg ttggagtatc cagggtcgta ggtcatgagg ccgaaatcat
     7321 cttcgtcgac cttgatctcc ctgaagtcca tcgcccggac tgtgccgtct tttatctcga
     7381 cgtcatagct cttgcccgtt cgattgtccg tgaccgatag actctcggtc cccgtggtag
     7441 tcgtaccccg cccgttaccc gtgctctgcg actcttcggt caacctgtgc ccctttctct
     7501 tgcacgtgcg ttaattatat atgctcctgc cacatatgtg actgtgtcga aacgtactgt
     7561 gaggtgccgg tattatcacg caccggagga aaaacggaaa gtaatgggcg cgaaccggcc
     7621 gcaagggggc gaaaccttcc tcttgcgtct gcgtataggc ggttagccgc ggtgaagaag
     7681 gagcgcacat gaagactgat tcgaggaact acgggatcca gcacgactac ggccctcgac
     7741 tcgaggcgtc gagggccggt gtgatgagcg tcggggggga gcgcacgtgg tcggtgttgg
     7801 cccatttgag cacgttcttg aacctcttta ccggatttct cgggccggtt gctgcgttcg
     7861 tgatctggct ggtttacaag gacgactcgc ctaccgtggc caggcacgcg ctcagatcca
     7921 tcttctacca ggttgcctgg ctcaccgcca tctttatcgg gtggaccgtc acgttcgcgc
     7981 tgatggcgat cctcgtcggc ttcctgctag tgcccattat ggcagtggtg acgctcgcac
     8041 ctttcgtgca ggcctcctac gaggcgtaca tggcataccg gggcggtagc cgtcgctact
     8101 attgagatac agatttcggg ctacgggcgt tgggttgggt gcctgctcta ggagggatcc
     8161 tctagctgga aagttacctc cacgttgacc ctgtaggcct tgatgttgcc atcctctatg
     8221 ctgacgttca tgtccttgac ccacgcgcct tctacgcccc tgagcgtttt ggtggcgcgg
     8281 gcgacggcca cgttgatggc atcctcgaaa ctggtctcgg acgtggcgct caactctgtg
     8341 acccgggcaa tactcatgat gttcgcctcc ttgccaaacc ctagattgcc gcttatctgt
     8401 gttgtatgta gcctttatga gtggagatca aacttcatgg gtcagggggc tccaaccgta
     8461 tcactcgtgg ctgaagtctg ctccttaggg agcggggaac ccaacaacac ccgtcgaagc
     8521 gtctcggcat gttgcctcga ctcccgctcc cgctcctcga agcgtgccgc tgccatttct
     8581 tgtcctgcct cgtgtgccct tgcggcaagc cttcgtgcca tggcagcatt ctcttgcaac
     8641 ttgttgagcg cgaagtacaa cgtatcctcg agtttgtcac tgttcttctc gagcacgtcc
     8701 tcggcgctgc cgtatgcgtg gccaacgcgg catctgtagc gcgacaattc cccttcacgg
     8761 atctcatata atggccccga acaatccgga cacgtgagac tcgagacatt tccgggtcgc
     8821 tcatcgctgt tcaaggtggc cgggtctagc tcggacatct tttgttcgag ctccatgtcg
     8881 tccgacatga gaaatcctcc ttgctctact ccttccttac ttctcgtctc tgtagcgagt
     8941 cggctcaata gggccgcgat cccgtctaat ggtaggcaat ggtccacctc cacgtactcg
     9001 agggcgctct tcggcattct ggcgaacgag gcctcatgtg ggtcctggac gatcgccaca
     9061 ccgccgcgct gcttgacggc ttgcagaccg gctgttccgt cgccatccgc tcccgacagc
     9121 accacgccga ccacgcgggc gccataagcg agggcggccg tacggaacaa gggatcgatc
     9181 gcgggccgat gatagttctc cttgggcccc cgcgtgagtc tgacccatcc ttccttgact
     9241 tgcagatgcg agcctggagc agcgacgtag atgcgaccgt gctcgatcgg atctccatca
     9301 cggggattcg tggcggcgag tggccctgcg cggctcaaga gatgtggcag ggtattcgga
     9361 gcctcttccg ggtagtgcac aacgacaaac accgccatag gcagatcttg cggcagggcg
     9421 ccggccagcc ggataagtgc ctcgacacca cccgccgaag ctccgatgac agcgatgtca
     9481 tgcgcggcca tgagcctcca aactaatgtt ggaactgtag cacagcctat gcgatctcca
     9541 tatcaggctg tcgttcgacc gatgcagccc tcgcagaaga gccatccgac ctcacggtcc
     9601 cgagcacgat ccaatatcgt tcggggttcg aaaaggcgcg aatattattt cgataggaac
     9661 gtgtctaatg ccttgccaag cctgtttgtt ggtcgctatc agcgcaacgc cgagttcttg
     9721 gatgggttgg tcctatccac gcggctcggt gaaaaacgtc cctatgaggc ctgctcctcc
     9781 tttgtgcgta ccaccagcac ggggcagtga gcgtgcctca cgaccgagtc ggaggcgctg
     9841 cccatcaagg ccctcctcat gccacccacg ccccggctgc cggtagcgat aagatccgct
     9901 cctatctcct cggccaaccc aactatctct tcgtccggac gccccatcct cagataggcc
     9961 ctcgagacgg ttaccccggc cgcctcgatc tcgcccacct gtccctctag tagccgccgg
    10021 gcttcctcct ggagctcttc gtatcgggcg cggtacccat gcctttcagg atggaagacg
    10081 ggctctacct ctcctacgtg gaccacgtgg agctccgagt ccgtacgctc agcaagctcc
    10141 gcagtcgtct gggcggccgt agatgcctct tcggagccat ctacggccag caggatcctg
    10201 cccggcagga agtctctcgg gctctcaccg tctcgcacca ccagcacggg gcagtgggcg
    10261 tggcgcacca ctgaatcgga gacgctgccc atcagggctc tccggagccc gccccttccc
    10321 ctgctgccca ccactatcag cccggcccct atcgtttcgg ccaacccgac gatctcctgc
    10381 ggcactcctc cctccaccag gtgcgtctgc gccagcgtcc cgccgaccga gcgtagcttc
    10441 tccgcctccg cgtcgagcag ctccctggcc ctttgttggg agatccgctg gacgtcctcc
    10501 tccagcacct ggtcttccag cgcaactcca tcgaatctgg tggcttcggg gtaggcagcg
    10561 taccaggggg agatgccgaa gacgtgtacc acgtgcaact cagatccggt cttaccggcc
    10621 aattcggccg cgctccgggc ggccaggtcg gcctcttttg aaccgtcagt ggccaaaagg
    10681 accttggtgg ggaagatgct catgtaaggt gtcctcctcg ctcgaagctt gtcccgcgcc
    10741 tacatgatgc cagccgcacg tacccggtgc gacctctcag aacaccgtgc atgcgacgtt
    10801 cggtatctct ttggccccca cgacgccagg tgttttgcac ctgccttagg ggaacgagcg
    10861 cacccacttc aagaccacgg gtaggtccgt ccgttggtag tagaccgatc ccctgcacac
    10921 ggagtaaccg aacgaggtga cgcccactat ggtctgctgg tcgggaacga acagcggtcc
    10981 gcccgaatcg cctttacagg aaccttctcc cttgccgttt acgaagttga cgcccgtggt
    11041 cttgacgaac atgccgctta gggcgtcgct cgtgtcgagg agcctgaccg tcgcgctgtt
    11101 gcggtcgcgc ggatagacca gctgcggccg cgacgatccg ccgccgattt cgtatccggt
    11161 cgtgccgtag cccacggcgg tgaggcgttg gcccacctcg agggtgtcga ccaggcccgc
    11221 aaccggcagt gccccgtagg acgccatcct gaccggctcg tcgaggacgg ccacccctac
    11281 gtcgtaacgg atgtcgcggg ccagactgcc cacgatctcg ggacgtcctg acggtgtgca
    11341 ttcacctttg ttctcagaat tcgggaagca gaacgtggga tgcgtatggg gcgttgcctt
    11401 tatggtcttg gatgctttcg gcttgtaggt cgggtcgaaa gagacgtagg tcggcagatc
    11461 ctctcttgtc aggaggtccg tgcaatgccc cgcggtgagg aagaccttgg gagaaattag
    11521 ggtgcccgag cacactggga tgcgttgccc tttgaactct ccggatgggg gcacggtcac
    11581 tagcgcaccc acaaagcggt gggcatcagg acccctgtct ggctcgccgt ttataacggc
    11641 ctgtgccacg ccaccggcca gcaggacggc cagagccatc gtcgccagca caagcactac
    11701 tcgccgcatg cgcccttctc ctctctttct cctacacgcc cccatcggag catctgcata
    11761 caaggtgaca aaactatgaa cccggggctt ctacttccga gaacgaagcg ttgaaaccgt
    11821 ctgaaaactc caatacgtgc ccggaacgtg acctcacagg acatcgaagc ggcgctgtgc
    11881 gacctgtttc ggccccgatg tgtcggcctg attcgcgttt ggagcgttac ccaaactccc
    11941 ttcggagtgt ttctctacgc gctcggggaa tagaggcagt caggttcttt cggaccttgt
    12001 gcttcacggt cccctagggc ccgtagagcg ggatggtggt ctggggtggc gtggtgtacg
    12061 tgggtagagg aggcagcgcc acctcggagc agtagacgat ttccacgggc gactcacaac
    12121 cctttatgac ctcgccgttc aagacgttgc cggcgatatc cacgttccaa aggtcgggtc
    12181 tgtccggccg accgctgtcc gtcgcgcgta tcgcaacgcc ctcagaatcg ggtaggtacg
    12241 caacgccagc gataactgcc gccccgaaca cgttgttgcg caccagcgcg ttctgtgcat
    12301 cgtgtatgct cacaccgcca cgtaccgcac cggtagagtt gccgtgcacc tcattgttct
    12361 cgatgagcgc ttcgccggag tcggcgacgt cacccacctc ctcgaagcgg atacccgccc
    12421 gtccgttatt gaccaccagg ttctcgtgca cccagaatcc gttcggatgg ctttcggagt
    12481 cgtggcagta cacgtcgcac cacaggccgt tgccctggtt gtcgtggatg taggagttcc
    12541 tgacctcgac ctcgttgatc gccttcaagc cggaacccgt aaagcccagg aaattgggat
    12601 cctgggtggt gtcgtcgagc tcgatgcggt cgaaggtgcc gtgggcgttg gtgatgcccg
    12661 ccgcatcggt gcccgttatg tgtaccgcgt agaggctgct ggtattggac gcctttccca
    12721 tggcgagtcc ggagcccgtg ccggcgagcg gggagccgcc cgcatactgt cccgtgccac
    12781 cgacgatctt gacccactcc actcgaaccg tgcccttggc gcggatcacg tttcttaggc
    12841 cctcactacc cacgatggtg actgtgggct ccgggtcgaa ggcggggtag cgctctatga
    12901 acgagccggc cgggcccgcg agctcatcac cttcgttgag caccaccatc gtatcgacgg
    12961 ggtaggtgca cggtccctcg agctggaatc gggtccctaa ggtcgggtcg tcggcgttga
    13021 cgatagcgtc gaggtcttgg ccgcacggca cgacgactac cctatccgca cctatcacga
    13081 agagcgccga caagaccact atcgaagtaa tgcagatcag ttcgaatagg cgcttcactc
    13141 cttcaccttt cgtcggatgg ctaggcacac gcgtctaccc cacgctttat aggacccaca
    13201 ttactacaac gggaatgaat tcgcgatgaa ctttcgggaa agaaatgtcg cttttgcaca
    13261 ttttctttcg gaacttcttg cgagccaggc tcgaaatcgt cctagaattc gacctctgaa
    13321 accgtccgga aagcctcgga ggcatctgtg gcgtaccgtc cggagcactc agaaggcggt
    13381 ctctcgactc atatcgccgt cccaatgctc gccagatcca cgctcggagc gttgcttcct
    13441 cggctccggt cacgaccgcc tctagagctt gccgtcgatc ttctccagga gggtcttgcg
    13501 gttcttgttg ttcttctcgt aggaccggac ggccctgatc tcgtctttgg agaggccttc
    13561 gatcttcttc tctacctccc cgacgctcag gtcgtcgtag ccgcttatgg ggacttcttt
    13621 ggcgttcttg cgggcttcct tggcctcttc ggtgttggag acgtactgct ggcctttctt
    13681 cgagccttcg cgtttcttct gctccgtcac ctccttctcc tcctcgctca tgttctccca
    13741 ggccttcttg ggaaggtagc gtttggtctc gccgctatcc tggcgggcgt cggcctcgcc
    13801 ctccttcgtc tgccattcct cctcggtcca ctcttccagg tccttctggg attggccttt
    13861 ctcgcccttg tagccgcccc gcttctcgta ttcctgggtg aggagctggg acttgcgggc
    13921 gctccactga ccctccttgc cacccttgtc ggaggctttg atctcctcct tgacgctctc
    13981 cctcagttcg gggtgggtgt acttctcttc gtagttctgc ttcgccacct gttcctcctc
    14041 ggtcgtcggg ccgtcgtcac tttcgtgcct ccatcgcgag aactagtact atgatcgttc
    14101 ggcgccatcc gtattcattg acacacgggg acgcgaccat gaacaggaat ggcccgaaag
    14161 gtgtaagccg gtgcaactct tggacctttt cttagcctca tgcccctgct ttgtgtccgt
    14221 tctcacccct gctgcgctag cgtggctaga atctgggatg agttttcgag aattactcta
    14281 gttcaagtac gcctgcgcat cgctttggcg caggcggtgc gcgagttcgt cgatgtcgag
    14341 gccggcgggg tcgacaatgg ggagttcggg gaggtttcgg ggcggtgggt acttctgtcc
    14401 gatgccgcag tcgaagacga cgatatcgtc gtgccgtccg atctctcccc tgtcgcgcag
    14461 gagggggacg gcggcgaggg ccgctgcgga ctcggggctg acgtagccgg ctcccatgcg
    14521 tcctgcgaac atctgcatct cggagatgga gtcttcgctc accgcgacgg ccgtgccgcc
    14581 tgattctctc agggagtcca ggatcaggaa gtcgccgacg gcggaaggga cccgaatacc
    14641 ggaggccctg gtcttcgcat cctgccaggg ctcggcgaac ctgtctcccg actcgaatgc
    14701 acggacaatc ggggcgcagc cctccgactg cacgctcacc atcctcggcc ggtcgggccc
    14761 gatcagtccc atcgtttcca gttcctggaa ggctttccac atccccacga ggcccgtgcc
    14821 gccgcccgtc gggtagacga tcaagtccgg taggcgccag ccgcgctgtt cggcgagctc
    14881 gaagcccatg gtcttcttac cctcgacccg gtaaggctct ttgagcgtgc tcacgtcgaa
    14941 ggctccaatc ttcggggcga gggcggtcga tatcttgccg cagtcggaga taagacctcg
    15001 caccaggacg agccgcgccc ccatcgcgag cacttcctgc tggttgacgg gcgtagcgtc
    15061 ctcgggcgtc agcaccatga cctgcgcccc ggcggcggcg ccgtaggcag ccagcgcgcc
    15121 cgccgcgttc cccgccgaag gcgcgatgaa cgaggtcact cccaactcta cggcccgcga
    15181 gaccgcgacg gccatcccac gggccttgaa cgagcccgtg gggttcaggc cctccgcctt
    15241 cgtggagaga cgcgggaccc cgagtgcctc gccgagccgt ggttgcgcca agagcggcgt
    15301 atccccctca ccaagataca cgacgttgct cttatctctc accggcagga gctctgccca
    15361 gcgccacagg ccgcccgtcc ggcgctcggc aagcgatttc cttctcagag ttcgggcggc
    15421 cttctcgatg tcgtagcggg cgagcagagg gcgttcgtcc actgggtcga gcccgatcaa
    15481 ctcgtccgca gggtagctct cccccgaaag tgcgccctcg agatgcgtca gcgtagagcc
    15541 cgtatcgttc aaatcaaccc ttcctaagac cgccggcatt ctacctacgt gttgcgacga
    15601 ttgcctcctg gtatagatgg tatttccaga atggtcgatc aagggtgctt ggtcttgaac
    15661 gaaggtggcc cagacatggc aacgatctag aacccttgga cgattccctg caacacgtgg
    15721 gtagtacgac ccgcctataa caccgatagc tccagaccgt ctctgatatg gtgcttttga
    15781 aagcattgaa tagtgttttg caaagcacta tactgtgatc atgtacgagc agatcaaccc
    15841 gttcgtttat ggccgccctg tttacgacga ggaagcgctg gccgaccgtg cgaccgagaa
    15901 gaggcggttg atctcggacg ccaactccgg gcagccggtc atactctacg cccccaggcg
    15961 gtatgggaag acgagcctgg cgcgcgtggt ttccgggcgc ctgctagaag atcacctcgt
    16021 cccttcggta tacgcggact tctggggagc cgccagcatc tctgacgtgg tagaggtgct
    16081 gggccgggcc tatgctgtgg cttcggttcg gcagagagcc aggcgtttct ttgccgatct
    16141 gctccggtcg gtaggattca gcgtggagtt tgccggaacc gcggtgagcg tcagttacga
    16201 gggcaagggc cgcaacgagc agcagcgcgc ggccttgatg aggctactgg acgtgccgca
    16261 acagatggcc gtccgctctc catctcagag gcgcctcttc atggttctgg acgagttcgg
    16321 ggaggtgttc aacgtgccgg gtgagccgta cgcactgatg agaaacgcgt tccaggcttc
    16381 gccgaacgtg tccttcatct ttatgggcag caagcgttct ctaatggacg cgctcttctc
    16441 cgaacggcat cacccgttct acaacttcgg acgcaggatg agccttggca ggctgccctt
    16501 cgaagagttg ggagccttca tcgaaggcag gttcgcaagc ttcggaaagc gtgtgacgcc
    16561 agaagcggtg gatctgctgc tctccctcac cgacggccat ccccaccgta gccagcagtt
    16621 ggcctaccac gccttcgaac tagcttccgg agaagccgat gaggagtcca tactatccgc
    16681 caaagaagct gcgtttgacg agaccgccga cgagttccgg gtactgctag accgcatgtc
    16741 taccgctgcg cgggccgtct atcttgcggt atgtaaggat cccaccacgg agatgacctc
    16801 cagggtttac ctccaacgcc acggcatcaa gagcacgggc tcactgcgaa gcgccatccg
    16861 caccttaacg gacgctggag acctggagca gaccggggcc aagagacccg tcccgaccga
    16921 cccgctcctc gccgcctggg tgcgggaacg catgaacagg ggttaagagc ggccggcacc
    16981 gtctcagccg actcgcgccg cgcgcatcgc ctccaggtaa tccagcgtct cttcgaccga
    17041 gaccacatcg ccgtacttgg cgtctatgtc gtagaggttc gccttgtggg cgtcggggtt
    17101 gcggtcgcct acggcctcgc tggggactat ggggcggaag ccgtactgga gggcgtcgac
    17161 ggccgtcgcg cggacgcatc cggaagtgga ggcgccggtg atgatgaggg tgtctacgcc
    17221 ggctgcggtg aggaggctgg agaggccggt gccgaagaat cccgaggcga agagcttgtt
    17281 gaggacaggc tcggtctctt cggaggctat gcgggggtcg atctcggccc accgggttcc
    17341 ggcctgcagc gtcaggagcg cgggcacttt atctatgaag gcggctgcgg tgagtttgtc
    17401 cgactcgcgg taggcaacgg tggtgaagac gacggggatc tctgcccgtc gggctgctct
    17461 caggagcttc tgtatctccg agatggggcc ttccaggtcg caggccaggg gagattccgg
    17521 atctgtgaac ccaagggtca tgtctatgac ggcgagggcg ggacggcggc cgaaaccgcc
    17581 gcgtccgccg aacccttcta tgtgcgccaa agtgtcaccc cccgttgccc cgggctttgc
    17641 cgagttcgcc cgagagccac tcgttgaact tgcgacgggt ctcggcgatc atgcgccgct
    17701 gctcatcgag gggcgggaga tttccatggt cgaaccccgg tgactcgcct cgggtattcc
    17761 ttatctcctc gcgcttcgat tctgtcgctc ccgcgtcgat ggagccgtcc tcgtttacaa
    17821 cgacgccgta gacgctctcg gcgacctcgg ggctgatgag gttccagcgg acgtctttgg
    17881 ctatcgcttc gggttcgcgc tccagcgggt cgccgacgcc gcctgcgcct gccgtctcga
    17941 agatgaggcg atctccctcg aagacaggca cgtcgcgcac tttggccggc atgtcgacct
    18001 cttcgccgtc tgccctgact agcgtcttct tcgaggccgc gccgggcttg ccgccgtcaa
    18061 cgccgtaggg gtaggtgtga tgcctgtcgt cctggtaggt gatgcggccg tcggccagaa
    18121 aacggtagac cttctttatt ccgcagccac ccctgaactg gccggcgccg caggagtctt
    18181 tgcgggccga gtactcctcc acaatcatgg ggtagtacgt ctccatgtac tcggcgggga
    18241 cggcgaggaa ttcgggccac caggagtgtc catcgaggcc gtccttgccg gggatggcag
    18301 gaattccacc gtaaaggatc tccatggtct ggaacgactc gccgtcttct tttacgccgg
    18361 agtagacgaa gttggggctc gtcccgtagg agcccgagac gttgaagccg atggccttcg
    18421 cgtagactgc gccgagaacg tcgaactggc gggccatgac gacgaggcgg ttgctcaagg
    18481 gcgccggctc cctcggccgc aggacagtgc cgtcggggat gttgaccttg atgatgtcgg
    18541 cgtagccgtc gttgaagagg atcgtcgggt ctacggcaga gatcaggaat acccccgcga
    18601 acatctggaa catctctttg tttaggagga agttgaccgg tcctggtacc tgcgcgtctg
    18661 tgcccttcca gtcgaggtgc agcgagtcgc cctcacgcca catcgcgagc ttgagcttta
    18721 tggggccgtt tcccagcccg tcgtcgtcgg tgtagtcctc gaactcgaaa cgctcgcctt
    18781 cgggtatgag ctggcggatc aagtttatga tgctcgtgcg cgtccgggcg aggagcgcat
    18841 cgcaggcctc gaggtaggtg tctttaccga agcggtcgca gatgtccttt acccgctgtg
    18901 cgcctgcggc cgtgccggcg gctatggcca tgacgtcggc gcgggtctcg cgcggggtgc
    18961 gggagttgtg gcagaagaag cggagtacct cttcgttcag gacacctccg tcgtagagct
    19021 tgaccggcgg cagcctgact ccctcctcgt agatggagcg ggcgtcgatg gggatggagc
    19081 ccgcggtcga gccgccgacg tccatgaggt tgccccactg caaggcgtaa ccaatgtgat
    19141 ctccttcgta gaagatcgga cgcacgagca ggatgtcagg gaggtgggag gtgctccctt
    19201 ccatcatgta cgggtcgttg agggctatga cgtcgccgtc cttgagatcc tccaccgtgt
    19261 agggggagtt ttcgagaacg gtatccacgg ggctcccgaa ctggcccacg accatacggc
    19321 cctggcggtc cgctatgagg gggaactcgt ctgcctgctc ccggatgacg gggctcacgg
    19381 cggtggtgac gagcacccgg tccatctcgt ggcggatgtt tttgagggcg ttctcgatga
    19441 tgtcgagggt aacgtggtct atgtcgtgat cccggacgaa atccggctcg gcctgctcgt
    19501 gcaacaccat ctcttatgcc tcctcgatca ggatgttccc gaagctgtcc acggcgcccc
    19561 tgtagcccgg ctctattacc gtagtcgtgt cgtcctgcac gacgatggcg ggaccggcta
    19621 cggcgtttcc ggggcgcaaa ccggcacggt cgtagatcgg ggtctcattc cattcgccgt
    19681 cgaagtacac ccgctctctg cgcgtgatcg cgccagacgc gtcctgcccg gcgccgtcct
    19741 cgctctccag gaggctgacg ccctttatgg tgccgcgccc gacgacgcgg acggtggcga
    19801 tctctaacgg agagtctggc aggtcgaagt tgtagcgctg gcggtgggcc tcctcgaacc
    19861 gtccgcgcaa aaaggcgctg ctcccatcgt cgagttcctc gagctcgaag cggcaaggga
    19921 tctggatgtt ctgcaggtag tagcggcagt ccgcataaag gtcgaacagc ttcttgtctt
    19981 cctccacccc ttcgccttcc agccactcgt cggcctgcgc gatgagtccc tcgatcgtcg
    20041 actttagatc ctgtgcccgg gtctcttctg cgacgcgcag gtacgtctct gggaactcgt
    20101 tctggatatc cgaggagagg aacccgaagg cgctcatcac acccggcgtc ggggggacta
    20161 tcacggagcg ggagtggatc aggcgcccca gcgcgttcgc gtgcattggg ccggccccgc
    20221 cgaaggcgac gaggccgaag ttcctcgggt cgtggccgcg ctcgacgctg accacgcgca
    20281 gggcggcgtg catgttctcg ttgactatct cgatgatcgc ctctgccgcc tcctcgacgc
    20341 ccatcccgag ccgctcggtg atggtcccca cggcctccct cgccgcctct tcgtccatct
    20401 ccagcgtacc gccaagggcc acgccgggcg gtatgcggtg cagcacgacg ttcgcgtcgg
    20461 tcacggtcgg ggccacgcca ccgcgcccgt aggccgccgg ccccggatcg gcgccggcgc
    20521 tatccggccc gacctgcagg gctccgctcg ccgagacgaa cgcgatggaa ccgccgccgg
    20581 ccccgacgct gtggacgtcc accgcagggg acttgaactg gtagtacccg agctgtatgt
    20641 cgcggcgcac gggcgtctcg ccgttcaggc agagcgagac gtcggtcgag gtaccgccca
    20701 tgtcgaaagt aaggacgtcg gggaccccga tctcacgcgc caggtaagcc gaacccgtga
    20761 ccccgcccga gggacccgac agggctatct ggatcgggct ctccgccacg gccccggtgc
    20821 tcatcgcccc gccgtcggac cggacgatgg agagagctcc cccaaagcgc ttctcgtcga
    20881 tggacttctc gaagttcctc atgtacagca tggtgcgcgg ctgcacggcc gtgttgacga
    20941 ccgtggtgag cgtccgctcg tactcgccgt actcctgccc gatatccgcg gagatggaca
    21001 cggagagatc cgggtacgcc ccgcgcacga tgtccctggc gcgccgctcg tgggaggggt
    21061 tcacgtacga gttgaggaaa ccgatggcca gcgactcgac acccctatcc accagccgct
    21121 ttaccgcctc gcgcacctcg ttctcgtcca acgctgtgag cacctcaccg ccggaactta
    21181 tgcgttccgc gatacctacg gtgtctgtcg ggtcggccgg cgggtcgggt ttctggaggg
    21241 ccatccagcc gtagagcggg ccgggggtcc aggcgcgggc gaggtgcagg atctgctcgt
    21301 gccccttcgt cgtcaggaga ccgacccgcg agccggtgtt ggtcaggatc atgttcgtca
    21361 caacggtcgt gccgtgaaag aagaggtgga gatcgggaaa agagatcccc accttctcgc
    21421 agaccgcttc tatcccctcg accacgccct tcgaaggatc atccggcgtc gagagcacct
    21481 tgccctcggt cagccctccc gtctcctcgt cgaagaccag cacgtcggtg aacgtcccac
    21541 cgacatctac cgcgacgttg tacatttcgc tcccttcggt cgcttagcat ttcagcacgc
    21601 gccctttcgg gcgcttgtca gcacactccg ccctttgggc ttcgctttca gcatgtcagc
    21661 caggactcga ggacgcgaaa gctggaatgc tgacaagcgt cagccacagg ctggagcgtg
    21721 ctgaagtgct gacaagcgga ggccgcatgg ccgaagcgtg ctgacttgct gaaagctcat
    21781 acgaaatttc ccgccttgta cacctgtccc ggtagctctt tgtcgagttg ccttgcgagc
    21841 caggttgcta cttcgatcag ggcgtcgaga tctattcctg tggtgtagcc catgttgtgc
    21901 aggaggtaga cgaggtcttc tgtggcgatg ttgcctgtgg cgcgaggggc gaaggggcag
    21961 ccgcccgtgc cggcgacgga ggcgtcgagc accgtggcgc cgctctcgac ggcggctgcg
    22021 gcgttggcta tcccggtgtt gcgcgtgtcg tggaagtggc aggcgaccgt aataccgtgg
    22081 gtcgcgactt cctctacgag atttcgcacc tggtttggta cgcccacgcc gatggtgtcg
    22141 gccaggacga tctcgtctgg gcccgatgcg gccacgctgg aggcgatcct gagtacgtgg
    22201 tcggggtcga cccgtccttc gaaagggtct ccgaaggcgg cggagagcgt gatggagacg
    22261 cgtactccgt ctagccgggc ccgttccacg agtcgcgcgg cgagctgcgt ggcgtcggct
    22321 accgtggtgt tctggttgcg cctggcgaag gtctcggtta cggggaaggc gtagcggact
    22381 tcgtctactc cggactggat ggcccgctcg tagcccttct cgttgagaac gaggccggcg
    22441 tagatcgtgc caggggaacg ctctatggcc gacattacct cttctgcgcc ggccatctgc
    22501 gggacgcgtt tcgggttgac gaaactggca gcctcgacgc gcgggacgcc ggcggccgcg
    22561 agcttctcgc agagctcggc acgaacttca ggggagagga tcttgtcctc gttctggagg
    22621 ccgtctcggg gtcctacgtc gactacttcg atgcgcatca gacgatgccc cgttcgcgca
    22681 gacggtcgag gtcctccccg gctatcccga gcaccttctc gaatacttcg tcgttgtgct
    22741 ggcccagctt cggtccggtc cagcgcactt ttcctggcgt ttcggagagg cggggaacta
    22801 cgttctgcat ggggaacggt ccgatctccg ggtcttcgac ggtgacgatg ttctggcggg
    22861 cggcgtattg ttcgtcggag agcatatctt cggcggtgaa gagcttccct gcagggacgc
    22921 ctgcctcctt catggtctcg agcacttctt ccgcgcttct ttggccggtc cagtcggaga
    22981 tcagggcgtc cagctcttcc tggttctcgc cgcgggcatg gtgggttgcg tactcctcgc
    23041 cctcggccca ctcgggatgg cccagcgctt tggcgaagcg tccgaaaacg gtgtcggcgt
    23101 tggcgccgat cacgacgtag tccccgtcgc cggtggggta agtgttagac ggcgccacga
    23161 agggcaggat ggccccggtg cgccctcgga catggccggc gagcgcgtac tccgggatcg
    23221 tgctctccat cagcgccagg acggcctcgt agattccaac gtcgactacc tggcccttgc
    23281 cgcctcgtgt ctcgcggttg tacagggccg tgaccgcgcc gagggcgccg aacgtcgcgg
    23341 cgagcgagtc gccgaggctg atgccgactc gcggcggcgg acggtcgggg taccccgtta
    23401 cgtgccttat accgcccatg gcctcgccga tggatccgaa acccgcctgg tccttgtatg
    23461 gtcctgtctg tccgtagccc gagacccgca ccatcacgag acctggattg ttcccgctca
    23521 ggtcctcgta ccccaggccc catttctcca gcgtgccagg cctgaagttc tcgacgacaa
    23581 tgtcggcttc ggagatgagt cgtcgggcga ggtcctgccc ttcgtcctcc cgaaggttga
    23641 tggtgacgga cttcttgttg cgggcgatga tgggccacca gagggtgcga ccctctttgc
    23701 ggtggcggcc ccacacgcgc atcgggtctc ccttgcctgg cggctcgagt ttgatgactt
    23761 cggcaccgaa gtcagcgagg agctggccgc agaaaggacc cgccagcagg ctacccatct
    23821 cgacgacgcg cacgccttcc agaggacgaa tcccgttgct tccctcagcc attcagatcc
    23881 tcctccaggg ctcctatgac gaagtcgcga ccatcgtata cgtgctcgcg catgatgatc
    23941 tccaccctgt cggcgtcgcc gttctctaga gattccagga tctggcggtg atagtggttg
    24001 gagatagtcc gctcgtgcgg ggtgtaccag aagaacgcct tgaacatgag cggtaactcg
    24061 acggtacggt ttatcaggtg ctccaggcgc ctgttgcggc tggcctggat gatggtcccg
    24121 tgaaactcct ggttgagcgc cacgagggtc cggatctcct cctcatgatc gtcgaaccgg
    24181 cctggcaatc cctccatctc cccggccagc tcctccaatc gttccagctc ttctttctct
    24241 atgcgtccgg ccgcgcgtct ggcggcgtgt ccctccagta cggcacgcag gtcgtagata
    24301 tcccacacgt cctcgatgga gaaagaacac accgtggccc cgcgattcgg cgctatctcg
    24361 acgagtccct cggcctcgag catggtgagg gcctgcctca caggcgtcct gctcaccccc
    24421 agtcgctccg ctagttgctc ctcgatcaaa cgctcatccg gaccgtactc gcccgtcaag
    24481 atcaaagccc gcaaccgctc cacaatcccg acagaccgcg accctcgccc agggccacgc
    24541 acagcatatc ccacgcaaac ctccgtcttc gacgcacgga gaaccccttg tatacaatct
    24601 taggctcccg ctagcgttgc gtcaagtatc tcaaatcgag ggacgggact caggcgaggt
    24661 cggcagtctc gcgcatgagc aggtgccagg gcgtaaggat ctcacagcct ttagatgtca
    24721 ccgctatggt gtgctcgaac tgggcggaga gccgcttgtc tacggtcacc acggtccagc
    24781 cgtctgccag atagcgtatc tcgggagtcc cctcattgat catcggttct atggtgaacg
    24841 tcatgccctc cctaaaacgc atgccgcgac cccgtcgtcc gaaatggttt atgtggggct
    24901 cttcgtgcag cttgtgtccg acgccgtggc cacccaggtc tcgaacgacc ccgtaaccgt
    24961 ggtgttccgc gagttcttgg atggccgctc cgacgtcacc gaagtgcttc cctggtccca
    25021 cctcggcgat gccggcttcc aggcactccc tggctacatc cagaaggctt ctggcacgcg
    25081 gcgagatatt gccgatcggg tacgtgtagc aagtgtcgcc gacccaaccg cccaacgtgg
    25141 cgccgacgtc tatacccacg atgtccccat ctcgcagtct cctcgggcct ggaatgccgt
    25201 ggactatctc ctcgttgacg gctgtacaga tggtgcctgg gaagggaggg acggagggtg
    25261 agggctggta gcctttgtag gtaggcaccc cgccctcgga gcggatgaaa tcctcggcga
    25321 ccctgtcgag ttcgcggagt ttgactccgg ctcggacgtg ctcgtgtagc aagagcagtg
    25381 cacgggcaac aaggtcaccc gcccgcctca ggcctcggat ctcgttcttg ctccgcagca
    25441 cgaccatggg tagcagctaa agtagcacaa actggccatg cagaggtgtc gtcgagccca
    25501 tcgacaaggc cgagtgccaa cgtaccatgg ctcgatagcg aagcagggtt ttaggtctcg
    25561 cgcacctcgg cgaggactgc gccgcctggg acgagggatc cttcttcgaa gggtagggag
    25621 tggacgactc ctgcgtgggg cgcggagacg ggctgttcca tcttcatcgc ctccaggacg
    25681 aggaggagtt gtccctcctc gacctcctgg ccctcttcga cgagcacctt cacgaccgta
    25741 ccaggcatcg gagccgtgag gctggcggcg gcatcggctc cgggccctgt ctcgtcgacg
    25801 gacggaggca ggggcttacg gagcgtatgg gtttctcctc ggagggagat gaggacctct
    25861 tcggggccaa ggattattcc agcctcgacc ggctcctcat cgatcagggc gcggatgcgg
    25921 gtgtctccgt ggtagaggat ctcgacgatg gactcatcgc ctccggcggt gatcctaaac
    25981 gttccaggtg cggcgcgctc ggcctcgacg cggtgctcat cgtagtcgag ggtggtcgcg
    26041 ccgaggagtc gccaggggcc tgccgcgaag gggtctgatg aggacgggat gctgtagagt
    26101 tccgccgccg aggcgagcaa catagcctcg cgcgggaggg gtttctcagg ctgaggctcg
    26161 gtgagatcgt atgtttcgag caaacggatc gtggtctcgc ccgcctcgaa cgcgggatgg
    26221 gtcgcgatgc gcctgaggag cgggaggttg gaggagggac caaggatctc gtagtccgtg
    26281 agggctcttc ggagccggcg caccgcagaa ggacgatcag gggcagagac gatcagtttc
    26341 gcgagcatgg cgtcgtagtt tattggcacc tcatccccag cttcgtatcc ggcgtcattg
    26401 cgaacgcccg ccccctcagg tagggagaag gtttcgagca ggccgcccga gggcataccc
    26461 tgctcgtcct cggcatagat gcgggcctcg atggcgctgc cccgcaaggt cacttcatcc
    26521 tgggtgagtg ggagaggtac attggccgcg acggagagct ggaggtggac caggtcgagg
    26581 cccgtaacga actccgtgac gggatgttcg acctgcaggc gcgcattcat ctccaggaag
    26641 tagtagtggt catcgtcgag gaggaactcc accgttcctg cattgtggta gccggcctcg
    26701 cgggcatagc gaacggcggc ggtgcagatg gagtcgcgga ggtccgggtc gagcgcaggg
    26761 gacggggctt cttcgatgac cttttggtgg cggcgttgga tcgaacactc ccgctcgaag
    26821 agatggagga tgttgcctcg ctggtccccg attacctgca cctccacgtg gcgtgggtct
    26881 tccacgagtt tctcgaggaa caccgatccg tctccaaagg aagcttcggc ttcccgacgc
    26941 gcccccctta ccgcctcgac gaggtctttc tgaagagtca cggaccgcat tccccgtcct
    27001 ccaccgcccg cgctcgcctt taccaggacc gggaagccga tgagatcagc ctccttcgcg
    27061 agccgctcct cgctctgatc ttccccgtcg tagcctggga tcgtcggcac gccggcccgg
    27121 cgcgccagtt ctttggcccg caccttgaag ccgacggcgt ccatcgcctc gggcgagggt
    27181 ccgacccagg ttgcacctgc ggagccgacc gccctggcga actgggcgct ctctgagagg
    27241 aagccgtacc ctggatgtac ggcttcggcg cctgagcgtt ctatcacctc gacgagccgt
    27301 ccgacattga ggtagctctc cggtgccgga gccgggccga gcaggtagga ttcatcggcg
    27361 aggcggcggt gcatcgcgtg ttcgtctgcc tccgagtaga cggcgatgct ctttatcccg
    27421 agctcccggc aggtgcggat gatgcggacc gctatctcgc cgcggttggc gacgagaagg
    27481 ctttcgaagc tcaacgagcc cttctccaac tcgggtcgcg tttctccaga aaggctgcga
    27541 gtccttcctg gccctcttcg ccggtgcgca gctcggagat gacacgcgcc gtcaggccgg
    27601 gcgcctccat cggctccgtg gtctcgagcc ggcgaagcaa ccctttgagg gtcgcctgcg
    27661 ccaccggtcc tccttcgaga agctctcggg ccttctcatc gactgcttcg tcgagtccgt
    27721 cgctcgaaac gagcctgtgc acgaggccga tctcccacgc ccgtttggca tagaaccttt
    27781 cacctgtgag gaacaaagat ctggcgtgcg agaccccgat cttgcgtacg acgaacggtg
    27841 agatcgtggc cggagcgatg ccgagcctta tctcggagaa ggcgaacctc gcctcggaat
    27901 ccgccacggc cacgtctgcc acggcgagta gaccggcccc tccacccatc gcggcgccct
    27961 ggaccttcgc caccacgggc ttcggacact cgtctatggt ccagtacatc atagccagcc
    28021 gtcttgcgtc ttccagattc tcctcgtaag agagtcctgc agtctcccgc atatacccga
    28081 tgtcggcacc ggcgcagaac gagcggccct cgccggcgag aaccacgacc cgcacccgat
    28141 cgtcatcgga gatctcctcg aagcggcgcg tcaactcctc gatgagatca gcgttcagcg
    28201 cgttgtgtga atcgggcctg tttatcgtca cggccgccag ggggccactc ctggacactt
    28261 tcaacaggtc aggcatcggt accccaatct ctgcacgacg gcattactcg aattctccta
    28321 gccaacctat ctctccttca ccggtccagg aagatagcgc ctcggcgagc gcccggggcc
    28381 gcacgtcgag ctccgtcagg ctggtcagat ccacccagac cggcctgtag gttcccttgg
    28441 ctgtcgtccc cgacgtgatc atctcggtac cccttcccgt gccgaacgtt cctcctacga
    28501 tactcgcaag gtagtatcgc tgctcgcggc catcacggtg agccacggag atcaggtccc
    28561 caagctctac gccaacgccc aactcctcgt gcgcctcgcg cacggcggcc tgctgcggac
    28621 tctcgccact ctcgaccctg ccaccgggga acttgaagta agtgtgccta tcgcgcacgc
    28681 gttcgatcag ggccacccgc ccgttctcca cgataatgac ggcggcgcgc actctcacat
    28741 cctgaagatc ccgtagcgcg tctcctccgc aggcgcgttc gcggcggcgg agaggccgag
    28801 ggcgaggacc atacgggtat cgacggggtc gatgatcccg tcgtcccaga gccttgcggt
    28861 ggagtggtag gggttgcctt cgtcctcgta cttttcgagg atcggggctt tgaagtcttc
    28921 gcgctcttcg tcgctcatct ccttgtcctc acgccccagg ctctcttcct gcaccgtgag
    28981 aaggacgttc gcggcctgtt ggccgcccat cacgctgatg cgggcgttgg gccacatcca
    29041 caagaagcgc ggcgagtagg ccctgccgca cataccgtag ttgccggccc cgaaggagcc
    29101 gccgacgatc acggtgaact tcggcacgct ggcgttggcg accgccataa ccatcttggc
    29161 cccgtctttg gcgatgccgc cggcctcgta ctccctgccg accataaagc ccgtgatgtt
    29221 ctgcagaaag acgagcggga tgccgcggga gcaacacagc tctatgaagt gcgcgccctt
    29281 gagcgcgctc tcggagaaga ggatgccgtt gttggccagg attccgacgg ggtggcccat
    29341 gatactggcg aagccacata ccagggtctc accgtagagg ggcttgaact cgtgcagatg
    29401 actaccgtca actgtcctgg ctataacttc cctaacgtcg tagccctgcc tgtagtcgct
    29461 cggaacgatg ccgtagagtt cttccggatc gtaaaggggc tcctcgggct cctcgacggt
    29521 ccagggcaca tgcttctccc ggttcaggtt ctctatgatg gagcggacaa tggcgagggc
    29581 gtgctcgtcg ttctcggcga agtggtcggc gactcccgag aggcgggtgt ggaccccggc
    29641 gccgccgagc tcttccgcag atacttcctc gccggtcgct gccttgacga ggggaggacc
    29701 gccgaggaag atcgtacccg tccccttgac tatcacgact tcgtcgctca tcgcggggac
    29761 gtaggcccct ccggctgtgc aacttcccat cacagctgct acctgaggta tacccttcgc
    29821 gctcatctgc gcctggttgt agaagattcg gccgaagtgc tcgcggtcgg ggaagacctc
    29881 gtcttgcagc ggcaggaatg cgccgcctga atcaacgaga tagatgcagg gcaggtagtt
    29941 ctgctcggct acttcctgcg cccgcagatg ttttttgacg gtcatggggt agtaggtgcc
    30001 gccctttacg gtggcgtcgt tggcgacgac catcacctcg cgtccggaga cgcgtccgac
    30061 ccccgtgact atcccagcag ctggaacgtt tccgtcatac ataccaaatg ccgccagggg
    30121 cgagagctcc agaaaggccg tgcctgggtc gaggaggcgt tcgatcctct cgcgtaccgg
    30181 cagctttcca cgctctctat gccgcctctg cgccttctcc cccccacccc gtctggcctc
    30241 ggcggagtgc atgcgaagcc cgtccacgag ccccgagaac gcctcggcgt tcttctcgaa
    30301 ggctccgctc ttcgtatcta cgatggttcc tagcttgctc atcgctcctc gttcgggtgg
    30361 ctcgaccctg gggagcatgt tagctacggc tcactgcctg cgccaccatg cgatcaggag
    30421 attgtgctga aggtgctgcc ttcgacgttc ccggtacgcg cccaatctcc agcgctgcga
    30481 gagctatggt gggatcagtg ctggcggtcg agagacgcgc tgctccttcg ccctcacccg
    30541 acttcgagca cgatgagcag gtctcccgcc tcgacgttcg tgcccgaggc cacggccagc
    30601 cgttctacgg tcccgcccgc cggggcccgg atggccgact ccatcttcat cgcctcgatg
    30661 ctcccgatct gctgcccgcc aacgatctct tcgccttcgt cgaccgtgag cgtcaccacc
    30721 ccggtcatcg gtgcggccac gtgtccgtct tcgccgggga cagccttctc gcggaccggg
    30781 acctcgggtt gcagccaacg gtcctgggcg tctattggcc ggggctggcc gtttagcgtg
    30841 accagcaagg tcctaatgcc acgcccgtcg gcctccgtta tggcttcgag tcgtacgtaa
    30901 agccggacgc cgggctccag gtccacggcc agctcctcgc cgggctccag cccgtaaaga
    30961 aacgccctgg ttggtaccac cgacacgtcg ccatagcgtt ccctgaccgc ggcctgctcc
    31021 tttgcgggtc ctggcaacat caggcggttc agcgccggcc tgcggttctc gccacccagg
    31081 gccgtccgat cttcggcgga gagagcatca tctatggggg actcgtcgcg gccggcgagc
    31141 gcccgggaac ggaacggctc gggccatccc cctggcggca cgcccaactc tccgcgcagg
    31201 aacccgatga cgctgtcggg caggtcgtgt gaggcgggat ctgactcgaa atctgccagg
    31261 gggatgttcg cggagagcag atacagggcc aggtccccga cgaccttgct ggttggcgtc
    31321 accttcacga ggcgcccgag caacgtatcg cagcgggcat agagcgcctc gacctcctcg
    31381 aaacgctcgg cgagccccat agagagcgcc tgctggcgca ggttggagag ctgtccgccg
    31441 gggatctcgt gccggtagat ggtgcctgtc ggggaacgga gtcccgcctc gaaaggggcg
    31501 tacagcgtcc gcacggcctc ccaatagggt tcgaggtccc ccagggcctc gagtgagaga
    31561 ccggtcgcac gatcggtgtg gtcgctggcc gccacgatcg cggccagcga cggctggctc
    31621 gtcatgcccg agagcggcgc ggcggcgccg tctatggcgt ctaccccggc ttccaccgcc
    31681 gccaggtagg tgccgagttg cccccccgat gtgtcatggg tgtgcaggtg taccggcaga
    31741 tcgaactcgc tcttcagggc cgagatgagc tttcgcgccg ccggggcgcg caccaggccc
    31801 gccatgtcct tgatgcaaag gatgtgcgac ccggcgtcga ccaattcctc ggccaaccgt
    31861 agatagtagt caagggtgta gagatcttcg ttcgggtcgg agagatcgcc ggtgtagcag
    31921 acggctccct ccgccacagc cccggcctcc agggtggcct cgatggcggg gcgtatgtgg
    31981 tccacgtcgt tattggcgtc gaagatgcgg aagatgtcta tgcctgactc atgtgcctcg
    32041 tctacgaagg agcgcaccac gtcgggcggg tagcgggtgt agccgacggc gttctggccg
    32101 cgcaagagca tctgcaaaca gacgttgggg aggcgctccc tgagacgggc cagcctctcc
    32161 cacgggtcct cgtgcaggaa cctgagcgcg acgtcgaacg tcgccccgcc ccacacctcg
    32221 gcggagaaga gttggggcat ggtgcgggcg acgtgcgggg cggcggcgag catgtcgaag
    32281 gtccgcatcc tggttgcgaa caacgattgg tgggcgtcgc gcatcgtcgt gtcggtcacc
    32341 tggagagtat cggtctcgcg cagccagcgg gcgaggcctt ccgggccgag gtccaccaaa
    32401 cgctgcctgg agcccggcgg tgggtctccc tgcgccagcg gcgggagctt ggtgcgcggg
    32461 tcgggtaccc ggggcggcgg cccgtgcggg cggttgactg tgacgtcggc gagcagggaa
    32521 aggaggcggc tcgcccggtc ggcgccggcc gagacggcgg tgaggtgtgg acgctcgtcg
    32581 atgaacgagg tggtcgcgcg tccggcgagg aaatccgggt cggagagcac cgcccgcaaa
    32641 aaagccatgt tcgtcgccac gccgcgcacc ctgaactcgg ccaaagcgcg gcgagcgcga
    32701 acggctgcgg aaggcagatc cgcgccgtgg gcggtgacct tcaccaagag cgagtcgaaa
    32761 tacggtgtga tctccgtgcc cgcgtacgtg ttgccgccgt ccagtcggat gcccgcccca
    32821 ccgggagagc ggtacaccga gatacggccc gtgtcaggcc tgaagccctg ggcagggtct
    32881 tcggtcgtca cgcggcactg caacgccacg ccgcgctgcc ggatgcggtc ctgggcaaga
    32941 tcgagatccc gcagcgtctc gccccccgcg atacgaagct gggcgtggac caggtccaca
    33001 tcggtcgttt cctcggtgac ggtatgctcg acctggatgc gcgggttcat ctcgatgaat
    33061 acgtgatgcc cgtcctcgcc gacgaggaat tcgaccgtgc cggcgcccag gtagccgacg
    33121 gcgcgcccga agcggaccgc ctcctcgcac agacgctcgc gcagccgcag tgtgaggttc
    33181 ggcgccggcg ctatctccag caccttctgg tggcggcgct ggaccgagca gtcccgctcg
    33241 aagaggtgga cgacctcacc cgccgcgtcg gccagtacct ggacctcgat gtgcctgggc
    33301 ctaatgaggg cctgctccag aaagaccgtg gggtcgccga acgcggcctc tgcctcccgc
    33361 gccgcgttcc ttaccgaccc cttcagatcc tcgggccgct cgaccagccg caatcctcgc
    33421 cccccgccgc cgcctgccgc cttcacgaaa accgggtagc ctatgctctc ggcgactgac
    33481 gccgcctcgc cgggatctgc cacagacccg gaggctttca gtaccgggac gcctgcctcc
    33541 tcggcggccc ggcgggcgcg cagcttgttt ccggtaagct ccagcgcgtc ggacggcggc
    33601 ccgacgaaga cgagcccggc ctcctcgcag gccctggcga ggtctgcgct ctcggagagg
    33661 aacccgtagc cggggtagat ggcgtcggcc tcgacctgga gggctgtttc tacaagagtg
    33721 tcgacgtcca ggtaggcgcg cacaggatgt cccgcctccc cgatttcgta tgcttcatcg
    33781 gccttctgcc ggtgcaacga agcccggtca tcaggggtgt agacggcgac cgtgcggatg
    33841 ccgagctcgt aggcagccct gaacgcccgg atggcgatct ccccgcggtt cgcaacgaga
    33901 accttttgca gcatcacgac cacctctccg gccgaaactt actgtgcgac caacctctcc
    33961 tgcgcccctt ccgcgctctc cgccaggagg gagcgggcga tgatctggcg gtttatctcg
    34021 ctggtcccct cccctatctc gttgatcttg acctgccgcc agtagcgggc cacggcgcta
    34081 gtctccataa agccctcgcc accccagatc tggaccgcct ggtcagcggc ccgcttggcc
    34141 gtctctgaag cgaacacctt ggacatgctc gcctcccgcc cgaaggggcg gccctggtcc
    34201 ttgagccacg cggctttgag caccatgttt cgcgccagct ctatctccat cgccatgtcg
    34261 gcgagcttga actgaacggc ctggaactcg gagatggaac gcccgaactg ctccctctgc
    34321 tgcgtccgcg ccagcgcctc atcgaaacag gcctgcgcga ggcccacgga cagggcggcg
    34381 accgcgaccc ttccggcgtc gagcgtcgcg agaaactggt tgaacccctt tccgcgctcg
    34441 ccgagcagat tctcctccgg tactctgcag tcctcgaaga agagcgggtg ctggtcagag
    34501 gcgcgccagc ccagtttttc gtaggatggt ccgcgagtat atcccggagt gtcctggggg
    34561 atgaggatcg cggtgagctc gggtgtaccg ttctcgcggg ttccggtcct cgctatgatc
    34621 gtgacgccgc cggtgatgtt ggttccggag ttcgtgatcc attttttcga tccgttgacg
    34681 acccactcga ccccatcgag ttccgccttc gtgcgtgttc ctcccgcgtc tgtgccggct
    34741 ccctcctccg taaggccgaa ggcccagagg ttttcgcccg tggcgcacct ggagaggaac
    34801 ttctccttct gttcttgtgt gccgaaggcg gcgatggggg cgcagcccag ggagatgtgg
    34861 gcctccatgg ttatcccgac cgaggtgtcc gcgcgtgaga tctcctccag agctaaggtg
    34921 taggcgacga aatccccgcc cgatccgccg tacccctcgg gaagcgtaag acccatcagg
    34981 ccgaggctgg ccatctctgc gactatgtcg taagggaacc tctgttcgcg gtctagctcg
    35041 gcggcgcggg gccggatctc accctgggcg aactcgcgcg ccagatcccg ccaccggcgc
    35101 tgttcatgtg ttggatcgaa gttcacggaa atcctcctgc tccctcggat ttgggacatg
    35161 atagcagcag aatttctggt tcatggagat acctttcgag gaaatataca gtgcactcag
    35221 aaactaatta ctcacacggg cgtattagat tgtacaatta ggcacacgag accgacctga
    35281 aggaggcaag gatgctgggg gcgttaatct tctggatcat agtgggcctc gtcgcaggcg
    35341 cgctagccaa gttcattatg cctggtgacg atcctggcgg gtttattatc acgatcattc
    35401 tggggatcgc aggcgctatc gtaggtggct ttttggccag cctgatcggt atcggcggtg
    35461 gtggctttat ctggaccaca ttgatcgcca ccgtcggcgc gatcatactg cttgcgatct
    35521 accgggcgtt cgcgggcagt agagcagccg ggtaacaaag acgggtccga gggccgggat
    35581 cagaagtccc ggcccacttc ttgctgctga ttcgggccgt tcgcgaacgg cccccctacg
    35641 gtattgttgt gtcctgtagg ccgtagcgtt cgacggcggt ctggcggagc ttgtatttct
    35701 ggatcttgcc gctcgcggtc atcggatact cttcgacgaa gtcgacgtac tcggggactt
    35761 tgtagtaggc gatgttctcg cggcagtagt tctgcacatc ttcggccgtt agcccggagc
    35821 cttcgcgggg ctttacggct gctgcgacct tttctccgta tttctggtct gggacgccgt
    35881 agacctggac gtcggagacc tcggggtggg tgtagaggaa ctcctctatc tcgcgcgggt
    35941 agacgttttc gccgccacgg atgatcatgt ccttcgcccg gcccgtgatc ctgacgtacc
    36001 catcttcgtc catgaccgcg aggtcgccgg tgtggagcca tccctcgtcg tctatgactt
    36061 cgctggtttt ctcatccatc ttgtagtagc cgcgcatgac gtggtaaccg cgggtccaca
    36121 actcgccctg ctcttccggc ccggcgtcct cgtccgtctc cacgctgacg atgcggacgt
    36181 cgacgtcggg gagcttgcgt cccacgctcg atacacgcaa ctccagcggg tcgtcggttc
    36241 tggtctgggt gatgacgggg ctcgactcgg tctggccgta ggcgatggtg atctccgagg
    36301 cgcccatcac gtcgacgacc ttcttcatga cctccatggg gcagggtgag ccggccatta
    36361 tgccggtgcg taaagaaccg gtgtcgtatt gttcgaagtc cgggtgctcc agctccgcaa
    36421 taaacatcgt cgggacaccg aggagcgccg tgcaccgttc ctggtctacg gcctgcaaca
    36481 ctctctcggc gtcgaagctc tcgacgggga ccatcgtccc ctcgtgggtg accgtattga
    36541 gggtgccaag cacgcagccg aagcagtgga agaacgggac cggtatacac accctgtctc
    36601 cgggcccgag cttcatgcac tcgccgatgt agaaggcgtt cttgacgatg ttggcgtggg
    36661 ttagctgcac acccttcggg aagcccgtcg tccccgaggt gtactgcatg ttgataacgt
    36721 cctcggcatc gagagacgac tgacgctcgc gcaactcttc gtcggagacc tcctctgcct
    36781 tctccataaa ctcatcgaag cctatgacgg gcaggccttc gggcgaatcc ccgcccatca
    36841 ggacgacgtg cttgaggaaa ggcagttcct ctatcgataa ctcgccttcc gcggcctcgc
    36901 cgagctcggg gacggcgccc tgcaggatct ggaggaagtt cgcatcaccc gctccctcgg
    36961 tcaggaacaa gaccgcggcg tcggattgct cgagcacgta cttcagctcg tgcgacttgt
    37021 aggcggggtt gatcgtaacc agcacggccc cgactttgcc cgtggcgaac tgcaggatca
    37081 cccactctgg cacgttctgg ccccacacgg ctacgtggtc gcccttatct ataccgagag
    37141 ccatcaagga ccgggcgcac ttctccacga ctgccttgaa ctccgtgtag gtgtagcgca
    37201 ggttcctgtc cgcgtagacg agggcttcat cttcggggcg tcgtccggcc accagatcca
    37261 gcagcccgcc tacggtcaga caatcgatat cctcggggcg catactttcc tcctcgttcc
    37321 ggtccccacc gataccaggc agtctagcaa tcggggcagg ctacgcaagc cacctaccct
    37381 atcatggcag gttcatgtga atgcgtgcgg cacgacggag cccgccatcg cccacaggcc
    37441 tcatagcctc ctggtttccc gcctgcgcgg gaatgacgta tgggagggtc tcccatgcgt
    37501 tcgcagcacg gacgctgtcc gttccgacgg actcctcatc acttccctac tcgtcgtggc
    37561 gtgcggcgtc ggactcctgg ggcaccagca cgtggagggc attctgggcc acggagaagt
    37621 cttgtggggt catggccacc acttccccgt ctatattgac cgcgagatct gggtcggtct
    37681 cgatctggac gcgcgaggtc ctgaagtggt gtacgccttc catcttgatg aagtcgccgc
    37741 tcttgaggta cctggcgacc ccgatgaggt ccctatgccg gccgaggtct atggcgtaga
    37801 tgtcgaggag cttgtcgtct atgcccgatt cgggggcgac gaccataccc ccgccgtaga
    37861 agcgcccgtt gccgacggcg acctgcagca gacggtcgta ttcgacgggc tcgtggtcgc
    37921 cgtcggggaa ggtcagcctt gcggagaacg gttcgtgcct gaggaacgcc ttgatggcgg
    37981 ccgcgggata ggccaaggga cccgtgctcc gcttcagcca cggcgaaagt gcccgtgtgg
    38041 cctccacccc gagcccgacg gaggcgacgt tcacgtagta gttgtcgccg gcgagaccga
    38101 gatcgatgtc gacgattttg ccttccgcta tcgtcttgca ggcaccctcg atgtcctgcg
    38161 gtatcccaag cgtcctggcg aagtcattgg ccgtaccgag gggaagtagg cccaggagcg
    38221 tgtcgtggtt ggccaggaag tcaacgacgg aactcacgga accgtcaccg ccgcccagga
    38281 tcagaaactt gtaacctgag ccgtcggaga ggacctcccg caccgtctcg gggagccgcg
    38341 cgggatctct tatggcgtag gttacaccga gggggacacc gagctgctga aggcggtcga
    38401 gggactcgaa gaacgtccgc tccccactgc gagaacgtgt gttgacgatc aaggcggcac
    38461 gctcggtcgt gacgtcgtta ctcatacgta atccggttga tcgctgctga cggtatgacg
    38521 agcctggagc agtggattcc cgctccccgc tttcgcgggg acaagcttcg cgagaatgac
    38581 gggagaggag gggcgtattt ggcactcgct catcatgaca aagagagtat acgagtcccc
    38641 tgcccacagg gtttcaccgg aaggcgactc gaaggcgggt cagtgcgttt gctcaaccct
    38701 tttcggttcg tggtcttcgg cggagcggta ggcggcaagg acgacctcca gcgttcgcag
    38761 gccatcgagc ccacttgcgc ccgcaggtac gccctcggcc acgccgcgca ggaagtccgc
    38821 cagcatcagc gcgtccatat cctcccccat gtatgaccac gaaggggcgc gggtttcatg
    38881 gtcgaacatc ctcaggggtt gcgcgaatgg gtccacgtcg aggactccgg aggtgcccga
    38941 gatcctgagc gtcacgtcgc cccaggtcgg gtagccgtcg ccgcgggacc agctcgggtc
    39001 tatggtggcg aacgcgccgt cggcgtatga gccgccttcg agatccaggg tcaggatagc
    39061 cgcgtcgtcc gcccctcctg caccgaagaa cgaatctacc tccgcgtaga cgcctttcac
    39121 ctcgacgtcg agcatccagc gaagcaggtc ggcgaggtgg acggtgtggt ccatcaccgc
    39181 gccgccgccc gctagctcg
//
LOCUS       DATMVF010000203         6990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_172298, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000203 DATMVF010000000
VERSION     DATMVF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172298"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..184)
                     /locus_tag="VN178_10245"
     CDS             complement(47..184)
                     /locus_tag="VN178_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81392.1"
                     /translation="MASEIWQFLMFAVGFFVVALLWLFVVYLPGRTMERWEDKRTNRK
                     E"
     gene            551..3796
                     /locus_tag="VN178_10250"
     CDS             551..3796
                     /locus_tag="VN178_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS81393.1"
                     /translation="MRIALLGGFRVSVGTRTIEEDAWHLRKAANLVKLLALAPGHLLH
                     REQAMDALWPDLARRAASNNLRQVLHAARRVLDPVVGSRYLASENGSLILGQEGLLWV
                     DAEAFGEAAIAARRARAPSAYEAAIEQYAGELLPGDHKETWTQDRREELRRLYLTLLL
                     DLATLYEGREEYERGIRVLRRALSEDPSREETHAGLMRQYAHCRRRREAVLQYELLVE
                     TLTREPGAETRRLYERIRAGSFPQESAGADGRESEDSGQHNLPASSTRFVGREREIAE
                     ARRMLSMTRLLTLTGAGGSGKTRLAVEAAGGLAGAYPDGVWMAELAPLSEPGLVPQAV
                     ARALGISEQPGRPLEGTLVEAMRSKELLLVMDNCEHLIQASASLAEELLSSCPRLRIL
                     ATSREPLGIGGEAVRQVGPLSLPETSDGQSTAESLMRCEAVRLFVERARVTVPDFGLT
                     EGNAGAVVRVCKRLDGIPLAIELAAAQLGDLAVEQVAGRLEVSLDLLQSSGRSAEPKH
                     QTLRSTLDWSYNLLGEIERASFGRLSVFAGGWTLEAAERVCSGSSIEQDEVLDLLGSL
                     VDKSLVVAAPAAVGEMRYRMLESIRQYAKEKLEEGDQDYEARVRHATFFLALAEEAQP
                     ELDGPQQRFWVERLEGEHDNLREVLSLVLEEGKGEPALRLGAALWRYWHHRGYLSEGI
                     RWMERVLAEGETAASQVRVRALEGMGWLTQIQGETGLAEAAYEEMLELSRELDDKGNV
                     ATALNSLGTLAVGEGDYERARVMLEENMTVLRELENERNAATTLKRFYAFNLLGSLAI
                     NEDNDYARGEALLEESLASVQEVGDTFLVGATLSNLGYTALMQGNYERAMMRCEEALA
                     LAYELGSAGVEIIPETLVNSGLVALLQGDYDRGRMSFEEALVMGRNAGRKATVINSLE
                     AMASLAGVLGEQTRSARLWGAAEAAREATAIVLPPGERTLHEPYLISARTRLGERKWE
                     KTLAEGRTMTLEEASEYALSKEKTAPPAAPVPERPPAGELMSSLTRREEEVATLVALG
                     FTNREISTRLSISERTAGNHVGRILKKLGLGSRTQIATWATEHGLSTHVPD"
     gene            4020..4697
                     /locus_tag="VN178_10255"
     CDS             4020..4697
                     /locus_tag="VN178_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81394.1"
                     /translation="MRRIVLMVAGVLLALAVASPMALAQAGQGEKASGETAELAATWW
                     QWALSKPFEGNPLIGGDPDYGEEQCDGQPVTGTPGDVWFLAGTLDGSEVERTCTAPAG
                     TQLFFPAGNIVFLITEPGETEQMARDYVNGYMDDVLADPSFKMRVSVDGKKIKRNQII
                     RSDSPLFTATVPEGGLLDAGSYDGVADGLWVTLPPLPEGEHEIHFQISAPNAGFSQDN
                     TYHLTVE"
     gene            4870..>6990
                     /locus_tag="VN178_10260"
     CDS             4870..>6990
                     /locus_tag="VN178_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWS81395.1"
                     /translation="MYIPAGFEDIDRSAAIRIRLLGGFRVSVGPRSIEGEAWRLRKSA
                     SLIKLLALARQHRMSRRQAMEYLWPESAGTTTSNKLRQTLHAARRALGTEGSGHLVSE
                     DGSLVLCPEAELWVDAEVFEEAALAARRSRKPAAYRMAIDLYAGELLPEDRYEEWAED
                     RRADLQRVHLALLTELAGLYEEQGEYDPAAETLRDVVAEDPTDEVAHARLMRLYAISD
                     RRGEALAQYERLRDVLSQHFDTEPAAETRMLQEDIAAGRYPAGRRAVPQPGEMFGAAD
                     HNLPARMTSLVGRERELVEVKRTLAMTRLLTLTGPGGSGKTRLALEVAGDLVGAYPEG
                     VWLVELAPLSEPGLVVHEVAGVLGVQERSGGPLLDELVDSLRSRRVLLVLDNCEHLVD
                     AVARLVGYLLRSCPDLRVLATSREALGIPGEVNWPVPTLSLPDPGRPATVEELEASGS
                     ARLFAERALQRPSAFALTLENAGAVAEVCRQLEGIPLAIELAAARVGVLAVEQISERL
                     SDSLGLLTGGGRTLTLRQRTLRGSLDWSYALLDEAEGKLFGRLAVFAGGWSLEATETV
                     CSGEGIAQDEVLDLLTELVDKSLVMVGSTANNVTRYRMLEPIRQYAKEKLEESGEADE
                     VRIRHAAFFLALAEEAEPELAGSRQDLWVGQLDTEHDNLRAALSWTLQRGEGEVGLRF
                     GAALWRFWHDRGYLSEGVRWMERVL"
BASE COUNT         1381 a         2052 c         2394 g         1163 t
ORIGIN      
        1 cacctcgctg cagacgcctg ccctccgctc ctccgtaacg ggttcttcac tctttgcggt
       61 tcgtacgctt atcctcccag cgctccatgg tacgtcctgg aagatacacg acgaagagcc
      121 acaacaaagc gacgacgaag aaccctacgg cgaacatcag gaactgccag atctctgacg
      181 ccaaaacctt cctctctacc ctcgtgaccg ttgctgtgca ggatacagaa agagtatctc
      241 acatcaggca tgcatccacc gtaagactat catcctggtc tcacggcacg ggggtagatc
      301 cctgggaccg gccggcggtt cagatggaga actcctaccc cgacaacgtg cacggcgccc
      361 gcccagaagc tatgatgtgt cgcttacacg agggagacga ccgagagccg ctttgctact
      421 ggcgcagggc ggcacccgtc ggcgggatgg cacttctgtc gagatcggta tggggatgtc
      481 gcaacatagt agaagaccgg cgcaggtaga gaacgagtct acaaacacta gtgtcaggga
      541 acccgaggtc ttgcggatag cgttgctcgg tggcttccgt gtctcggtag ggacaaggac
      601 catcgaggag gatgcctggc acctgaggaa agcggcgaat ctggtgaaac tgctcgctct
      661 cgcgccaggc cacctgctgc accgggagca ggcgatggac gcgctctggc cagacctcgc
      721 gaggagagca gcctccaaca acctgcgcca ggttctccat gccgcccgac gggtcctcga
      781 tccagttgtt ggttcacgat acctggcctc cgaaaacggg tcgctcatcc tgggccagga
      841 agggttgctg tgggtagatg cagaggcatt cggggaggcg gcgatagccg cccgtcgcgc
      901 cagggctccc tccgcctacg aagcggcgat cgagcagtac gctggagagc tgctccctgg
      961 agaccacaag gagacgtgga ctcaagacag gcgggaggag ctgcgccgcc tgtacctcac
     1021 gttgctcctg gatctggcaa cgctctacga aggacgcgag gaatacgaga ggggcatcag
     1081 ggtgcttcgg cgggcgctct ccgaagatcc atccagggaa gagacgcacg ccggcctgat
     1141 gcgccagtat gcccattgca ggcgccgccg ggaggccgtg ctgcagtatg agctgctcgt
     1201 ggaaaccctc accagagaac ccggagccga gaccaggcgc ctctacgaga ggataagggc
     1261 agggagcttt cctcaggaga gcgcaggagc cgacgggagg gagtcagaag actccggcca
     1321 gcacaacctg cccgcctcat cgaccagatt cgttgggcga gagcgtgaga tcgccgaggc
     1381 caggaggatg ctctccatga cccgtcttct gaccctgacc ggggcaggtg gatcgggtaa
     1441 gacgcgactg gccgtggagg cggcgggcgg cctggcaggg gcgtacccgg atggggtgtg
     1501 gatggccgag ctggcgccgc tctcggaacc cggtctcgtg ccgcaggccg tggctcgtgc
     1561 gctcgggata agcgagcagc ctggacgtcc gcttgagggt actctggtag aagcgatgcg
     1621 gagcaaagag ttgctgctcg ttatggacaa ctgcgagcac ctgatccagg cttccgcgag
     1681 cctcgctgag gagcttctat cttcgtgtcc acgcctgcga atactcgcca cgagccggga
     1741 gcctctgggt atcgggggag aggctgtcag gcaggtaggc cccctttccc tgccagagac
     1801 ttcagacgga caatcaacag ctgagagtct gatgcgctgc gaagctgtgc gactgttcgt
     1861 ggagcgcgcc cgcgtcacgg tgcctgactt cgggctgacg gaaggaaacg ccggtgcggt
     1921 ggtcagggtc tgcaagaggc tcgacgggat accgctagcc atagagctgg cagcagcaca
     1981 gctgggggac ctggcggtcg agcaggtcgc tggcaggctc gaagtgtccc tggatctgct
     2041 gcagagcagc ggccggagcg ccgaaccgaa gcatcagacc ctgaggtcaa cgctcgactg
     2101 gagttacaac ctcttgggcg agatagagag agcatcgttc gggcgactat cggtctttgc
     2161 aggaggatgg actttagaag cggccgaacg ggtatgttca ggctccagca tcgagcagga
     2221 cgaggtcctc gatctcctcg gcagtctggt ggacaagtca ctcgtggtgg ccgctccggc
     2281 tgctgtcggt gagatgcgct accggatgct cgagtcaatc cgccagtacg ccaaggagaa
     2341 gctggaagag ggcgatcaag actacgaggc gagagtccgg cacgcaacct tcttcctcgc
     2401 cttagccgaa gaagcccagc ccgagctaga cgggccgcag cagagattct gggtggagcg
     2461 gttggagggt gaacacgaca atctcaggga ggtcctctcg ttggttctcg aagaggggaa
     2521 gggtgaaccg gcgctgcgac tgggagcggc gctctggcga tactggcacc accgaggcta
     2581 cctgagcgag ggtatcaggt ggatggaacg agtgcttgcg gaaggtgaaa cggcagcatc
     2641 gcaagtacgg gtgagggcac tcgaggggat gggatggctg acccagattc agggcgagac
     2701 cggactagcg gaagcggcgt acgaagagat gctagagcta tcccgggagt tggacgataa
     2761 ggggaacgtc gcgaccgccc tcaacagcct gggcacactt gcggtgggag aaggcgacta
     2821 tgagcgggcg agggtaatgc tcgaagagaa catgacggtg ctcagggagc tcgagaacga
     2881 acgaaacgca gccacgacgc tcaagagatt ctatgcgttc aatctgctgg gatctttggc
     2941 gatcaatgaa gacaatgact acgctcgagg tgaggcgttg ttggaggaaa gcctggcatc
     3001 ggtccaggaa gtgggagaca cctttctggt aggggcaacc ctctccaacc tggggtacac
     3061 agcgctgatg caggggaatt acgagcgtgc gatgatgcgt tgcgaagagg cgctggcgct
     3121 cgcctacgag cttgggagcg ccggcgtaga gatcatcccc gaaaccctgg tcaacagcgg
     3181 ccttgttgcg ctgctgcaag gtgactacga ccgcgggagg atgtcctttg aagaggcact
     3241 ggtgatgggc cgcaacgcag gaagaaaggc gaccgtcatc aacagcctgg aggcgatggc
     3301 cagcctggcc ggggtcctcg gagagcaaac tcgttcggca cgcctgtggg gagcggcgga
     3361 ggcggcgcgg gaagctaccg cgattgttct gccacccggc gagcggacgc tgcacgaacc
     3421 ttacctgatc tcggcccgaa cccggcttgg ggagaggaag tgggaaaaga cattggccga
     3481 gggacggact atgacgctcg aagaagccag cgagtacgcc ctctccaaag agaagaccgc
     3541 tccacccgcc gcacctgtgc cggagaggcc accggccggc gaactcatga gctcgcttac
     3601 acgcagggag gaggaggtag cgaccctcgt cgccctgggc ttcaccaacc gcgagatctc
     3661 taccaggctc tcgatctccg agcgcacggc gggcaaccac gtcggcagga tactcaagaa
     3721 gctgggactg ggctcgcgca cccagatcgc cacctgggca accgaacacg ggttgtccac
     3781 gcacgtcccc gattagagcg acgttccccc gttcgcaaag cgcgttttag ggttcgctgt
     3841 ccctccagag cgctcccaat gctagtcgat ttcccggccg tggaccgata ttgagtagaa
     3901 atgctcacgc cttgagcctc ctcggctaac gcccagataa cgcccccttc ctaccctgat
     3961 tcccgtaggg aagcgaacgc aacccaaaac gcagaagggt tgtagaagga ggtagcgcaa
     4021 tgaggcgcat agtgttgatg gttgccggag ttctgttggc gctggccgtg gcctcgccga
     4081 tggccctcgc gcaggccggg cagggggaga aggcgtccgg ggagaccgcg gagctggccg
     4141 ccacctggtg gcaatgggcc ctgtccaaac cctttgaggg caatccgctc atcggcggcg
     4201 accccgacta cggcgaggaa cagtgcgacg gtcagcccgt gaccggcaca cctggcgacg
     4261 tgtggttcct ggcggggacg ctcgacggga gcgaagtgga acgcacctgc accgcgccgg
     4321 ccggcacgca gcttttcttc cccgcgggga acatagtgtt cctcatcacc gagcccggcg
     4381 agaccgagca gatggcgcgc gattacgtca acggctatat ggacgatgtg ctggccgatc
     4441 ccagtttcaa gatgcgcgtt agcgtcgacg gcaagaagat caagcgaaac cagataatcc
     4501 ggtcagattc tccactcttc accgccacgg tcccggaggg cggtctgttg gacgcgggtt
     4561 cctacgatgg agtagccgat gggctctggg taacgctgcc gcccttgcct gaaggcgagc
     4621 acgagatcca cttccagata agtgctccga acgccggttt ctcgcaggac aatacctacc
     4681 atctgacggt agaataggtc cagaacatcc agggagccag gtccctaatc ggggcctggt
     4741 tccttggacc ctggcgattc ccgcaagcga taagcccagg gaaatgagcg ggaggatctt
     4801 gccttccctg atgcattgac cggtgttcca atccctagca gaatatcgaa gagcgatcca
     4861 ggggatgggg tgtatattcc tgccgggttt gaagacatag atagatccgc ggccattcgt
     4921 atccggctac tcggcggctt cagggtctcg gtgggtcccc gaagcatcga gggcgaggcg
     4981 tggcgtctga ggaagtcggc gagcctgatc aagctgctcg ccctggcacg gcaacatcgc
     5041 atgtccagga gacaggcgat ggaatacctc tggcccgaat ccgcagggac gacgacctcc
     5101 aacaaactgc gtcagaccct ccacgccgcc cgcagggcac tgggcacaga agggtcgggc
     5161 cacctggtct ccgaggacgg gtcgctggtg ctctgcccgg aggccgagct ctgggtcgac
     5221 gcagaagtgt tcgaggaagc cgcgctcgcc gccagacgct cccgcaaacc ggcggcctac
     5281 cgcatggcta tcgacctcta cgccggggag ttgctccctg aggaccgcta cgaggaatgg
     5341 gcggaagaca ggcgtgctga cctgcagcgc gtgcacctcg cgctgctcac ggaactggcc
     5401 ggtctctacg aagagcaggg agagtacgac ccggccgccg agacactcag ggacgtcgtg
     5461 gccgaggatc cgaccgacga ggtggcgcac gcccgtctga tgcgcctcta cgccatctcc
     5521 gaccgtcggg gcgaagcact ggcccagtac gagcggctac gggatgtact ctcgcagcat
     5581 tttgataccg aacctgcggc ggagacccgg atgcttcaag aggacatagc cgccggcagg
     5641 tatccggcgg gacggcgagc cgtcccgcag ccgggggaga tgttcggagc ggcggatcac
     5701 aacctgcccg cccgaatgac cagtctcgtg ggccgcgagc gcgagctcgt agaggtgaag
     5761 cgaaccctcg ccatgacgcg gctcctgacg ttgacgggtc cgggcggctc tggcaagacg
     5821 cgcctggcgc tggaggtggc cggagatctg gtcggggctt accccgaggg cgtgtggctg
     5881 gtcgagctcg caccgctatc cgagcctggg ctggtggttc acgaggtagc cggggtcctt
     5941 ggggtgcaag aacgttcggg gggaccgctc ctcgacgagt tggtcgattc attgcggtcg
     6001 agacgggtgt tgctggtact ggacaactgc gagcacctgg tcgatgctgt ggcgcggttg
     6061 gtagggtatc tcctgcgctc gtgcccggac ctgcgggtcc tcgccacgag tcgcgaggcc
     6121 ctgggcatcc ccggcgaggt caactggccg gtgccaaccc tgtcactgcc agatccgggg
     6181 cggccggcga cggtcgagga gctcgaagcc tctgggtcgg cgcgcctgtt cgcggagcgg
     6241 gcgctccagc gtccctcggc cttcgcgctt acgctcgaga acgcgggagc ggtggccgag
     6301 gtctgcaggc aactggaagg tatcccgctg gccatagagc ttgcggcggc gagggtgggg
     6361 gtgctggcgg tagagcagat ctccgaacgg ctctccgact ccctcgggct ccttaccggc
     6421 ggcggtcgta cgctcacctt aaggcaaagg acgttgcggg gttctctcga ctggagctac
     6481 gccctactgg atgaggcaga ggggaaactg ttcggccgac tcgcagtttt tgcaggaggt
     6541 tggtccctgg aggccaccga aacggtgtgt tccggggaag gcatcgcgca ggacgaagtc
     6601 ctggatctcc tcaccgaact ggttgacaag tcgctcgtga tggtaggatc gaccgcgaac
     6661 aacgtgacgc gctaccggat gctcgagccc atccgccagt acgccaagga gaagctggag
     6721 gagagcgggg aggcggacga ggtgcggatc agacatgccg ccttcttcct cgccctggcg
     6781 gaagaggccg aaccggagct ggccggatcc cggcaggatc tttgggtggg gcagctggac
     6841 acggagcacg acaacctgcg ggcggccctc tcgtggaccc tccaacgagg ggaaggtgag
     6901 gttgggctcc ggttcggtgc cgcactctgg cgattctggc acgaccgagg ctacctgagc
     6961 gagggggtcc ggtggatgga acgagtactt
//
LOCUS       DATMVF010000204         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_621766, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000204 DATMVF010000000
VERSION     DATMVF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621766"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VN178_10265"
     CDS             <1..256
                     /locus_tag="VN178_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81396.1"
                     /translation="EISGGAQGRATYEWMEGGFFLIQRVDLQQHGQKIKGIEIIGHER
                     EFGAEPGEDIKSPFARCSRVGQTGKNRTNVGGSRTKWNCR"
     gene            304..786
                     /locus_tag="VN178_10270"
     CDS             304..786
                     /locus_tag="VN178_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS81397.1"
                     /translation="MHEWAFGEDERNRELLEEAMNFVGAIVAGRRTYDISVGWWGADG
                     PAGRARVPVFVVTHEVPDEHPENGIYTFVTDGIEGALDQAKAAAGEKAVAVMGGAETG
                     RQFIRAGMVDEISIHLVPVLFGGGTRLFEHLGAEHIQLETLEVIETPAATHLRFRVVE
                     "
     gene            962..1162
                     /locus_tag="VN178_10275"
     CDS             962..1162
                     /locus_tag="VN178_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81398.1"
                     /translation="MRRKFGKNGPGVGAMGLGCWAIGGPFEFDGRPAGWGEVDDGSED
                     ERLTHSKRATLVVDDGTVVFAR"
     gene            1688..2230
                     /locus_tag="VN178_10280"
     CDS             1688..2230
                     /locus_tag="VN178_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWS81399.1"
                     /translation="MEVLVVLLVSFVLLRGVGSLGVKRLASWRNAGLGALAIMFLFTS
                     TAHFSSMKHDLAAMMPEPLPDGLWIIYLSGFFEIAGAIGLLVPRTRRLAGICLVLLMV
                     AVFPANVNAALNGIPLGGEAPTPLWLRAPMQLLFIGMLWWTSITRGRTVPGTKQRRGA
                     PKERPTEVENRMQQPNESRI"
     gene            2292..2837
                     /locus_tag="VN178_10285"
     CDS             2292..2837
                     /locus_tag="VN178_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81400.1"
                     /translation="MARFSGSTATGAAAFKPGPLPGVPREVQASFLLWLTAVGAGIVE
                     TVIRVIGSLAGGPDSSGLLVRVIVYTVAVYVIAQMRLGKRWARITLTILLGGIGTLSL
                     VIDPISWLAAGNSLGEVFTQADLLFVFVAPIRAVHLAAVITALVLMFLPAANDFFHRA
                     SSAAGAPGSPTAAATREKTGR"
BASE COUNT          588 a          928 c         1111 g          563 t
ORIGIN      
        1 ggagatctca ggaggcgcgc aaggaagggc tacctacgag tggatggagg gcggcttctt
       61 cctcatccag cgcgttgatc tccagcagca cgggcagaag atcaagggca tcgagatcat
      121 cggacacgag cgggaattcg gggccgagcc tggcgaggac ataaagtcgc ccttcgcccg
      181 atgctcccgc gtgggtcaaa caggcaagaa ccggacgaac gttggcggct ctcgaaccaa
      241 gtggaactgt cgataaagag aggaagttga ggtgaacaac cgaccccacc gacgggcaag
      301 cgactgcacg agtgggcgtt cggcgaggac gagcgcaacc gcgagctcct cgaagaggcc
      361 atgaacttcg ttggagcgat cgtcgcaggc cggcgtacct acgacatctc ggtcgggtgg
      421 tggggggcgg acggcccggc gggacgcgcg cgggtcccgg tgttcgtcgt cacccacgag
      481 gtaccggatg agcacccgga aaacggaatc tacaccttcg tgaccgacgg aatcgagggc
      541 gcactggacc aggcgaaggc ggcagccggc gaaaaggccg tcgccgtgat gggcggcgcc
      601 gagaccgggc ggcagttcat ccgggcgggg atggtcgacg agatctccat ccacctcgtg
      661 cccgtgctgt tcggcggcgg cacccggctg ttcgagcacc tcggcgccga gcacatccag
      721 ctggagaccc tggaggttat cgagacgccc gcggcgacgc atctccggtt ccgcgtggtg
      781 gagtagccaa gttgggaggg acgaagcgtg gtggagatgt tcgccacgtt cgggaccggc
      841 cggtggtcag gtacgcggtc gggacccggg agatcggcgg ggcattcggg gcgcaggtac
      901 gccggttctc gcggctagcg ccgctggggt cggtctgccc gatacgaaga ggaaggtgca
      961 gatgagacgc aagttcggca agaacggccc tggggtgggc gcgatgggtc tcgggtgctg
     1021 ggccataggc ggccccttcg agttcgacgg gcgtcctgcg ggttggggag aagtggacga
     1081 cggttcggaa gatgagcgcc tcactcactc gaagcgcgcc acgctcgtgg tcgacgacgg
     1141 caccgtcgtg tttgccagat aggtagggac ggacggctcg attcggcggc actgggttgg
     1201 cttgaaatcc gcgaaaacac gtaccttgtc gcacggccgg aagtcgaccg ctgcaagcac
     1261 cggtcagatc tccgtgcgct ctggagccta tggggaggtc gaggatgtaa ctcgagggga
     1321 ggatctcgaa tctccacccc tgctccaccc ttcggccgat gacgtcccat ctggtcctcg
     1381 gatacaaaga tggtggcatc aaggaccact cgatagtcgg acgatcatgg tgaaggagcg
     1441 atccgatggt aggaaaggta gtgcgatgac ccttgggggc ggtggtcggg ctcctggcgc
     1501 tcgtcggtat cgcggcgtct gccacctact tcctccgcga gcccgccaac gaaggtttcc
     1561 tcaactaccc gaccatcgtg gccgtccacg tggtcctggg tgcagtttat atagagcgtc
     1621 cctcaaaggc gcagcggtat cagcagaagg cggatcaatc cgtcgtaaca agagggagta
     1681 cacgcacatg gaagttcttg tcgttctctt ggtttcgttc gttctcctcc ggggggtggg
     1741 ctccctcggg gtcaagcggc tcgcttcctg gaggaacgcc gggcttggag ccctggcgat
     1801 tatgtttctg ttcaccagca ctgcgcattt ctccagcatg aagcacgatt tggcggcgat
     1861 gatgcccgag cccctgcccg acggcctctg gatcatctac ctgagcgggt tcttcgagat
     1921 cgctggcgcc atcgggttgc tcgtcccccg gaccaggagg ctggcgggca tctgcctcgt
     1981 gctcctgatg gtcgcggtgt tcccggccaa cgtgaacgcc gcgcttaacg gcatcccgct
     2041 cggcggggag gcgcccaccc cgttgtggct gcgggcgccg atgcagctcc tgttcatcgg
     2101 gatgctctgg tggacctcca tcacgcgggg caggacggtc cccggcacaa agcaacggcg
     2161 cggcgcgccg aaagagagac cgacggaagt ggagaaccgc atgcagcaac cgaacgagag
     2221 tcggatctga ttgaaggagt cgagcagaag tgagggagcg cccatcgtca tcgaagaaag
     2281 gacgtggaga gatggctaga ttttcgggca gcacggccac gggagcggca gctttcaagc
     2341 ctggcccgct accgggtgtg cccagggagg tgcaggcgtc gttcctgctg tggcttaccg
     2401 ccgtcggggc ggggatcgtc gaaacggtca tccgcgttat cggctcgttg gccgggggtc
     2461 cagacagcag cggcctgctc gtcagggtga tcgtctatac agtggcggtc tacgtgatcg
     2521 cgcagatgcg tctcgggaag aggtgggcac gcatcaccct gacgatactg ctcggcggaa
     2581 tcgggacact ctccctggtg atcgacccga tctcgtggct cgctgccggt aactcgctcg
     2641 gtgaggtctt cacacaggcg gacctcctgt tcgtcttcgt tgcgccgatc cgggccgtcc
     2701 atctggcggc ggtcatcacg gcgctggtcc tcatgttcct gccggccgcc aacgacttct
     2761 tccaccgcgc ttcatctgcc gctggtgccc ctggatctcc gaccgcagcg gcgaccagag
     2821 agaaaacggg gaggtgagtc tatgcctctc ctccagcaga tgggcagctc gcgcttgctc
     2881 cgccggtcgt cgccgacggc cacgacaccg acgacgtttc ggacctcatc gagctgatgg
     2941 ggaggcgctg atgggttgcc catcaaagac tcgtggcggt tggcggggag agaaatcatt
     3001 ccgaaaggtg aatcttgtga ttggaagaat ctggcacggg tggacgaggc ccgagaacgc
     3061 agacgagtac gaacggctac tgaaggagga gatcttcccg gggatcgccg aaaggaacgt
     3121 ttccggatac agggggatcc aactcttccg aagacctatc gacagcggcg aggtggagtt
     3181 cgtcacgatc
//
LOCUS       DATMVF010000205         4524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_23648, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000205 DATMVF010000000
VERSION     DATMVF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23648"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VN178_10290"
     CDS             <1..885
                     /locus_tag="VN178_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS81401.1"
                     /translation="HGWPGSVIELLDTIGPLTDPTAYGGQAEDAFHLVLPSLPGYGFS
                     GEPAELGWNAGRTAQAWAELMRRLGYTRYVAQGGDVGALVTDTMGRQAPEGLVGIHLN
                     LLPAVLALADQLPAESEQERAALEAFNTFRESGFGYFLEMATRPQTIGYALLDSPVAL
                     AAWLLDHDTDSYYKISGAFVDGEPVGNLSRETIVDNITLYWLTGTGVSAARSYWEDGR
                     ALAAAQASGQAPPEVSVPVGFTTFPGEIWRTPRSWVDAVYPNLSYFNEVDRGGHFAAW
                     EEPELFSEEVRAAFRSLR"
     gene            967..1227
                     /locus_tag="VN178_10295"
     CDS             967..1227
                     /locus_tag="VN178_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81402.1"
                     /translation="MAPTEIMQKIEDLSEERERLLAREGSHHARVGDHRRLAEIDHSL
                     GVLWDLRRRELAGEYVRLDEDFLDRYVVSPGDDAPDDYTWAR"
     gene            1325..1930
                     /locus_tag="VN178_10300"
     CDS             1325..1930
                     /locus_tag="VN178_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HWS81403.1"
                     /translation="MTEGFFSRGFLGRRRSQDTELPPGQYLEDGFPVLSAGPTPRTPQ
                     EEWDFSIVGEIDEPRRWTWEEFSQLPSEEITRDIHCVTKWSKLGTSWEGVSVDTLLEG
                     VETAAEYVTAFCDGDYTTNLPLVDVTGGKAWVAFAYDGEPLEPEHGGPVRLLVPHLYF
                     WKSAKWVRGLQLTTYDIPGFWETLGYHNYGDPWREQRYWGD"
     gene            1935..2681
                     /locus_tag="VN178_10305"
     CDS             1935..2681
                     /locus_tag="VN178_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HWS81404.1"
                     /translation="MAVQLRRILWGLAEVIEVVPETPRTKSLLLEVPEWEGHEAGQHG
                     DVRLTAQDGYQTQRSYSIASAPEDERLMLAVDRLDDGEVSIYLTDVLMTGDKLELRGP
                     IGGYFAWEEGEGGPLFLVGAGSGIAPLMAMIRHRGAAGSDVPTRLLYSSRSYEEIIFR
                     EELETLASRDGALEVIHTLTRSRPEGWSGYDRRIDAEMLGEVGWSPEESPLVFVCGPT
                     SFVEGAADALVRLGHDPARVKTERFGPTGG"
     gene            2686..2958
                     /locus_tag="VN178_10310"
     CDS             2686..2958
                     /locus_tag="VN178_10310"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HWS81405.1"
                     /translation="MDELRLDGNAAAGTLGEVFSFEVTTAHYACENCGRADRIGAAMV
                     YEVRDLGTIVRCPSCDNALIRLAHNRGRYWIDLRGIRYLQVEDVAE"
     gene            3165..3890
                     /locus_tag="VN178_10315"
     CDS             3165..3890
                     /locus_tag="VN178_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HWS81406.1"
                     /translation="MLNVILFGLAASSALVIGAVAGAYWTPSKALLASALAFASGALI
                     TALAFDLFQESFERGGAWVSAIGVVGGAVSFVVADALLERYITEGGTNVMAPNSAFAL
                     LAAVTLDGIPENMALAVSLLETSGTGALALLFAIFFSNLPEALGGAVGMRQQGRSRGF
                     AIGVWSATAVVLAATVVVGNFALSGMGMEEGPLALLLSFAGGAVLASLADTLMPDAYR
                     EGGKWVALATTAGFLLSFLISEI"
BASE COUNT          805 a         1348 c         1549 g          822 t
ORIGIN      
        1 cacggctggc ccggctcggt tatcgagctg ctcgacacta tcggcccgct caccgacccg
       61 accgcgtacg gtgggcaagc ggaggacgcg ttccacctcg tgctgccgtc cctgcccggc
      121 tacggcttct ccggcgagcc cgccgagctc ggctggaacg ccggccgcac cgcgcaggcc
      181 tgggcagaac tgatgcgccg cctcggctac acgcgctacg tcgcccaggg tggggacgtg
      241 ggcgccctcg tcaccgacac gatgggtcgg caggcgcccg aggggctggt aggcatccac
      301 ctcaacttgc tccctgcggt gctggccctc gccgaccagc tgccggcgga atccgagcag
      361 gaacgcgccg cgctcgaggc gttcaacaca ttcagggaga gcggcttcgg ctacttcctg
      421 gagatggcca cgcggccgca gaccatcggc tacgccctgc tggattcgcc cgtcgccctg
      481 gcggcctggc tgctcgacca cgacacggac agctactaca agatctccgg cgccttcgtc
      541 gacggcgagc ccgtgggcaa cctcagccgg gagacgatcg tcgacaacat cacgctgtac
      601 tggctgacgg gcaccggggt ctcggcggcc cgctcgtact gggaggacgg acgagccctg
      661 gcggcagccc aggcgtccgg gcaggctcct cctgaggtct cggttccggt cgggttcacc
      721 actttccctg gcgagatctg gcggacaccg cgcagctggg tcgatgcggt ctaccccaac
      781 ctcagctact tcaacgaggt cgataggggc ggccacttcg ccgcttggga agagccagag
      841 ctcttctcag aagaggtccg agcggcgttc aggtcgctgc gttaattggc ggacgtgggc
      901 ggcgcgcacg gtcccggagt acgggaggtc cgtccccgca gcgagccccg aaaggaaggc
      961 gaagccatgg cgcccactga gatcatgcag aagatcgagg acctctcgga ggagagggag
     1021 agactcttgg cccgggaagg gtcccaccac gcccgagtgg gcgaccatcg gaggctcgcg
     1081 gagatagatc actctttagg cgtgctgtgg gatcttcggc ggcgggagct ggccggcgag
     1141 tatgtgagac tggacgagga cttcctggat cgctacgtcg tgagcccggg ggacgacgct
     1201 cccgacgatt acacgtgggc aaggtagcgt ttccggggga gaggaggggc gaggacgaga
     1261 cacgacagat cgacgggatt ttgtaagggc cagggtgtgg gtaggggatc gaggaggaga
     1321 gattatgaca gagggatttt tctccagggg ctttttgggc cgccgcagga gccaggatac
     1381 ggagctgccc cccggacagt acctggagga cggcttccct gtcctctccg caggacccac
     1441 gccccgcaca cctcaggagg agtgggactt ctccatagtc ggggagatag acgagcccag
     1501 gaggtggacc tgggaggagt tcagccagct tccctcagag gagatcacaa gggacatcca
     1561 ctgcgtcacc aagtggtcga agctggggac aagttgggaa ggggtctccg tggacacctt
     1621 gctagagggg gtcgaaaccg cagccgagta cgtcacggcc ttctgcgacg gcgactacac
     1681 gactaaccta ccccttgtgg acgtcacggg aggcaaagct tgggtggctt ttgcctacga
     1741 tggcgaaccc cttgagccag agcacggagg ccctgtccgc ctgctggtgc ctcacctgta
     1801 cttctggaag agtgccaagt gggtgagggg actgcagctc actacctacg acatccccgg
     1861 cttctgggag accctgggct accacaacta cggggacccc tggcgagagc agcgctactg
     1921 gggagactaa ggcggtggcc gtgcagctgc gcaggattct ctggggcctg gcggaggtga
     1981 tcgaggtggt gccagagacc ccgaggacca agagcctgct gctcgaggtc ccggaatggg
     2041 aaggtcacga ggccggccag cacggcgacg tgaggctcac tgcccaggat ggctaccaaa
     2101 cccagaggag ctactccata gcctccgccc cggaagacga gaggctcatg ctcgcggtgg
     2161 accgtctgga cgacggcgag gtgtctattt acctcaccga cgtgttgatg acgggggata
     2221 agctggagct gagggggccg atcgggggct attttgcctg ggaggaagga gaaggcggac
     2281 cgctatttct ggtcggagcg ggttcgggta tcgcgccgct gatggcgatg atccgccaca
     2341 ggggtgccgc gggcagcgac gtacccaccc gcctgctgta ctcctctcgc tcctacgagg
     2401 agatcatctt ccgcgaggag ctggagaccc tcgcctcacg ggacggtgcg ctggaggtga
     2461 tccacaccct gacgcgctcc aggcctgagg gttggagcgg ctacgacagg cgcatcgacg
     2521 ccgagatgct tggggaggtg ggttggtccc cggaggagag tccactcgtc ttcgtgtgcg
     2581 gccccacgtc gtttgtggag ggggcggcgg acgcgctggt gagattggga cacgaccccg
     2641 cgcgcgtaaa gaccgagcgc ttcggaccca caggaggtta gagctatgga tgagctcagg
     2701 ctggacggca acgccgcagc tggcaccctc ggagaggtct tctccttcga ggtaactact
     2761 gcgcattacg cctgcgagaa ctgcggaagg gccgatcgga tcggggcggc gatggtctac
     2821 gaggtgaggg atctgggcac catcgtccgc tgcccaagct gcgacaatgc cctcatccgg
     2881 ctcgctcaca accgcgggcg atactggatc gatctgaggg gcataaggta cctgcaggtc
     2941 gaggacgtcg ccgagtaaag cgaaatggcg atatcagagc caggtgatcc actagcacca
     3001 tcccgtgtag aacctggatc cgcttcgggg aaggtggaga acatcgtgag cgcacgctgg
     3061 ctgctgacga tcaacaccgg ttcgcccagc ctgaatgccg ccctgtagga gccgttactc
     3121 ttgtccgagc aaggctaccc tgcaaagcga cgttcgggtg agcagtgctg aacgtcatcc
     3181 tcttcggttt ggcggcctcc agtgccctgg tgatcggcgc cgtcgcgggc gcctattgga
     3241 cgccgtccaa ggcgctgctg gcctcggcct tggccttcgc cagcggtgct ctgatcaccg
     3301 ctcttgcctt cgacctcttc caggagtcct tcgagcgcgg cggcgcgtgg gtttccgcga
     3361 tcggggttgt cggtggcgcg gtgagcttcg tggtggccga cgcgctgcta gagcgctata
     3421 taaccgaggg tggcactaat gtgatggcac ccaactcagc gttcgcgctc ctagccgccg
     3481 tgactctgga tgggataccc gagaacatgg cccttgccgt ctcgctgcta gagacctcgg
     3541 ggaccggggc cctcgcgctt ttgtttgcca tcttcttctc caaccttccg gaggcgttgg
     3601 gcggggcagt agggatgcgc cagcagggac gctccagagg cttcgccatc ggagtgtgga
     3661 gcgccacagc agtcgtgctc gccgctacgg tggtggtagg caacttcgca ctctccggaa
     3721 tgggcatgga agaggggccg ctggcgctgt tgctctcctt cgccgggggc gcagtgcttg
     3781 cctcgctggc cgacacgctg atgcccgacg cttacaggga gggcggtaag tgggtggcct
     3841 tggccacgac cgccggcttc cttttatcgt ttctaatctc tgagatatag tgttggcggg
     3901 ttttcggcac ggcggaggcc cagtgcgagt taggctaggg gttacgctgg ggactccggc
     3961 cctccttctc gtccttattc accggagtgc gtagaaggag aattctcgga acttcccctc
     4021 ttgggtgttc tccaaacttc ccctgtgagg cattctcgga acttcgcctg gttacgttct
     4081 cggaagttcg tcctatagga gggctgaaga ccagggctca aaagtacgca aatttcgtac
     4141 gagggatccg tggcactagg ttatgatctc cttagcgctt tgatagcgct ccccggatag
     4201 aagggaggtt aagttgagcg gtcccgcatc ggatacagac taccggacca acccccgcgc
     4261 cctgaagtgg tggcgcctga agaatcgcta ttcccaaagg gatgttgagg aaaaaacggg
     4321 cgtgtcttac accacgatct cgaggctgga gaatggacac cggacccgcg ctaggttcgg
     4381 cacactcgag aagctcgcgc aagtctacgg ctgtacgata gaggatctga tcgttcttag
     4441 tagtcagttg ttagcgtagg gggtccctca ctccacgcgg ccccggtctg gagtggaggc
     4501 gtggactgag ggcgcgctat tcac
//
LOCUS       DATMVF010000206         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_668998, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000206 DATMVF010000000
VERSION     DATMVF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668998"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VN178_10320"
     CDS             complement(<1..791)
                     /locus_tag="VN178_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HWS81407.1"
                     /translation="MAKTDSKLESICEEVIARNPEEPEFHQAVREAVNSLGPVLDKHP
                     EFVEEKVIERLCEPDRQIMFRVPWQDDGGEIHVNRGFLIESSSALGPYKGGLRFHPSV
                     YLGIVKFLGFEQTLKNALTAMGIGGGYGGADFDPRGRSDREVMRFCQSFMDELFRHIG
                     ESTDVPSGDIGVGPREIGYMFGKYKRITNRYEMGALTGLDVGVGAPIRQEASGYGCAY
                     FMEEMLKTLGESFEGKTCVVSGSGNVAIYAIEKIQEEFGGKVVACS"
     gene            complement(947..2353)
                     /locus_tag="VN178_10325"
     CDS             complement(947..2353)
                     /locus_tag="VN178_10325"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS81408.1"
                     /translation="MNRLLKGGLAVACGGIAAGAVWRMLYHEPQPKYEPWERPPYDEF
                     PNRVLIVGGGFAGFTVAKTLAALTEDRDDVGIMVVSRENFFTYWPMVAGIISSDVEAK
                     NIAQPLRRSLIQYGASFRRAELEDVDLEQKVVKAKGGLEFSYDHLVLALGSDPAFFGV
                     PGVEEHCISMKGLADAERIRNRMIERFEEVTLEGDEADTVDYEPPKSKLTFVIVGGNA
                     TGVEIASELHTLAHETLALDYPTIDMNRVRIVLVDSNEEVLKELDNALRRAARRHLEE
                     LGVEVINNARAQEVTADRVILDDGEEIEAENVIWTAGNRASVKVDELDLPLQGEDGIE
                     TDEYMRVPGAENVWSIGDCAANVDKDGEPVPSNAQAATQEGEALAKNILAAIDGDELA
                     PFEYKPMGQLVELGSQFAVNEVMGVKFSGLPAALFWRAAYLFKLEIPENRARIAADWF
                     LDLFFHPSVTQIRGREGA"
     gene            complement(2640..3572)
                     /gene="mdh"
                     /locus_tag="VN178_10330"
     CDS             complement(2640..3572)
                     /gene="mdh"
                     /locus_tag="VN178_10330"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWS81409.1"
                     /translation="MAGKFKVTVVGAGNIGGTTALHLAMRNYADVVLVDIIEGLPQGK
                     ALDMLESGPILRFDANVIGTNDYEDTAGSDLAVITSGKPRQPGMSREDLLNENQEVVQ
                     SVTEQLVENSPECKIVVVANPLDAMCHVALETSGFPRERVIGMAGILDTARYRAFIAQ
                     EVGASVRDVFALVLGGHGDTMVPLPSMATVSGVSITELLPQERIDAIVERTRGGGGEI
                     VELLGSGSAFYAPAAAIAEMADAILLDQKRILPCAVYLQGEYGIEDLFVGVPVKLGAG
                     GIEDIIQLDLSNEEREDLEYSANDVRNMVEVLHS"
BASE COUNT          642 a         1278 c         1067 g          773 t
ORIGIN      
        1 gagcaggcca ccaccttgcc gccgaactct tcctggatct tctctatggc gtagatggcc
       61 acgttgcccg agccggagac cacgcacgtc ttgccctcga aggactcgcc cagggtcttt
      121 agcatctcct ccatgaagta ggcacacccg tagcccgagg cttcctggcg aatgggggcc
      181 ccaacgccga cgtccagccc ggttagggcg cccatctcgt agcgattggt gatgcgcttg
      241 tacttaccga acatgtagcc gatctcgcgc ggacccacgc cgatgtcacc cgacggtacg
      301 tcggtgctct cgcctatgtg acgaaatagt tcgtccatga agctctggca gaagcgcatc
      361 acctccctgt cggatctgcc cctcggatcg aagtccgcac cgccgtagcc gccgccgatg
      421 cccatggcgg tgagggcgtt cttcaaggtc tgctcgaagc ccaagaactt gacgataccc
      481 aagtagaccg aagggtgaaa gcgcaacccg cccttgtaag gacccagagc gctgctggac
      541 tcgataagaa agccgcggtt gacgtggatc tcgccgccgt cgtcctgcca gggcacgcgg
      601 aacatgatct ggcggtccgg ctcgcagagc ctctctatga ctttctcttc cacgaactcg
      661 ggatgcttgt cgagcacggg acccagggag ttgaccgctt cgcggaccgc ctggtggaac
      721 tcgggctcct cggggttccg cgcgatcacc tcctcgcaga tgctctcgag cttcgagtcc
      781 gtctttgcca tctcgctcct cctatccccg taggtctcca ggctctcacg agcacaacct
      841 agagagcctc gtgccttccc gtcgagcagc ttcccgaaac ctcttgcgag gcaccttttc
      901 ccacctcggc cgggctcgcc ttctcctacc tccggctacc tccggctcat gccccttcgc
      961 gtccccggat ctgggtaacc gacgggtgga agaagaggtc caggaaccag tccgccgcga
     1021 tgcgcgcccg gttctcgggg atctccagct tgaacaggta ggctgcccgc cagaagaggg
     1081 cggccgggag gccgctaaac tttacgccca taacctcgtt cacggcgaac tggctcccca
     1141 actccaccag ctggcccatg ggcttgtact cgaagggggc aagctcgtcg ccatcgatgg
     1201 cggcgaggat gttcttggcc agggcctcgc cctcctgggt agccgcctga gcgttggagg
     1261 ggaccggctc tccgtcttta tccacgttgg ccgcgcagtc gccgatagac cacacgttct
     1321 ccgcacccgg tacccgcata tactcgtccg tctcgatgcc atcctcgcct tgcaacggga
     1381 ggtccagctc gtctaccttg acgctcgccc ggttgccggc ggtccagata acgttctcgg
     1441 cctctatctc ctcgccgtcg tcgaggatca cccggtcggc ggtgacttcc tgggcccggg
     1501 cgttgttgat cacctcgacc ccgagctcct ccaggtgccg ccgggccgcc cgccggagcg
     1561 cgttgtcgag ctctttcagg acttcctcgt tcgagtccac caagacgatt cggacccggt
     1621 tcatgtcgat ggtcgggtag tcgagggcga gggtttcgtg cgccagagtg tggagctcgg
     1681 aggctatctc cactcccgtg gcgttgcctc ccacgatcac gaaggtcagc ttcgacttcg
     1741 ggggctcgta gtcgaccgtg tcggcttcgt cgccctccag ggtaacctcc tcgaagcgct
     1801 cgatcatccg gttgcggatg cgctccgcgt ccgcgaggcc cttcatgctg atgcagtgtt
     1861 cttcgacccc gggcacgccg aagaaggccg ggtcgctgcc caacgccagc accaggtggt
     1921 cgtaggagaa ctccagtccg cccttggctt tcaccacctt ctgctcgaga tccacatcct
     1981 ccaactctgc ccgccggaag ctggcgccgt actggatcag ggaccgcctt aagggctgcg
     2041 cgatgttctt ggcctctacg tcgctgctta taatccccgc caccatgggc cagtaggtaa
     2101 agaagttctc cctggacacg accatgatcc cgacgtcgtc gcggtcctcg gtcagcgcag
     2161 caagggtctt cgccaccgta aagcccgcaa acccgccgcc tacgatcagc acccggttgg
     2221 ggaactcgtc gtaaggaggc cgctcccacg gctcgtattt gggttgtggt tcgtgataca
     2281 gcatccgcca cacagccccc gcggcgatgc caccgcaagc caccgcgagg cctcccttaa
     2341 gcagtctgtt catgtcaccc tcctggtcgc cggttcccac ccgtaacaac gacaccatag
     2401 tagttgatta cctaccgtaa aagcaactat agagtgccac accgggcgct tctagcggga
     2461 ccaggtccac acgttctggg gatacatatg cgtatcctct aggtgggtgt tgctgttagc
     2521 aacctgccta ggaggagttc agaagtcggc ttgatctccg tgaggccggc tgtcgcccat
     2581 tcatgagaga ggggacctcg acaggtcccc tcctaagccg agcaggtctc acggaccgcc
     2641 taactgtgga gcacctctac catgttgcgc acgtcgttgg cgctgtactc gaggtcttct
     2701 cgctcctcat tcgacaggtc gagttggata atgtcttcga tcccgccggc gcccagcttc
     2761 accggtacac ctacgaagag gtcttcgatg ccgtactcgc cttgcaagta caccgcgcat
     2821 ggcagtatgc gtttttgatc tagcaggatc gcgtccgcca tctcggcgat ggccgcggca
     2881 ggcgcgtaga aagcgctccc gctccccaaa agctccacga tctccccgcc gccccctcgg
     2941 gtccgctcca cgatcgcgtc gatccgctct tgaggcaaaa gctccgtgat cgacaccccg
     3001 ctgacggtcg ccatgctcgg cagtgggacc atcgtgtccc cgtgcccccc caaaaccagg
     3061 gcgaatacgt cgcgtaccga cgcgcctacc tcctgggcaa tgaaggcgcg gtagcgggcg
     3121 gtgtcaagga tgccggccat ccctatgacc ctctccctgg ggaaaccgct ggtctcgaga
     3181 gccacgtggc acatagcgtc caaggggttg gccacgacca cgatcttgca ttccggggag
     3241 ttctccacca gctgctcggt gacgctttgg acgacctcct ggttctcgtt tagcaggtcc
     3301 tcgcggctca tccctggctg gcggggcttg ccgctggtta ttacggccag gtcggatcct
     3361 gcggtatcct catagtcgtt ggtgccgatg acgttagcgt cgaagcggag gatggggccg
     3421 ctctctagca tgtcgagcgc tttgccctgg ggtaggccct ctattatgtc gaccagcact
     3481 acgtcggcgt agttgcgcat ggccaggtgc agggcggtcg ttccgcctat gttgcccgct
     3541 cctacaaccg ttaccttaaa cttacctgcc atatacagcc ctcctcttac tcctcgtgcc
     3601 tcacgaacga tgagagacca gatacccact cttgccgttt ttgaacctcg cggcgaccag
     3661 gccgtgtgga tcttcagaac gcgcctctcc tccgacaaac gctactccag agcggtgcaa
     3721 ggaggaatac tgctctgtga cagtcaggta acttctgctc
//
LOCUS       DATMVF010000207         5638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_172713, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000207 DATMVF010000000
VERSION     DATMVF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172713"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VN178_10335"
     CDS             <1..730
                     /locus_tag="VN178_10335"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81410.1"
                     /translation="AIVTGGGGGLAEGICARLASDGAAIAAVDVSREKAERVADQVRA
                     NGTSAVAVEADVSDRRSVQAMVDEVVSGLGGVDILVNNAAVYPRRAWTEIEEEEWDRV
                     MAVNLKGYFLCARAVYPHMKERGRGSIVNVASITFFIGWTLLLDYVASKGGIVGFTRT
                     LAREVGPDNINVNAIAPGAFPTDAEKIHPDQEGYNRWVLEQQSIKRRGTPEDIGNLVA
                     FLASDSSSFITGQTIGIDGGWMMR"
     gene            741..1880
                     /locus_tag="VN178_10340"
     CDS             741..1880
                     /locus_tag="VN178_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWS81411.1"
                     /translation="MAEAATAKRERKEKLEDILVVDCDVHVHESPEALIPYCEMPWRK
                     ALETIKDVKEFYLDIPGFSPGTTVYEAKFPSGQEATRMVHSASQMREELDEIHVDIGV
                     LFPDHFLKIPVISQVDYASALARAYNAWLLAEWCSPENGLLGCILACPQDPVGAAHEI
                     EKYSDEKGMVGVFLPCAGLERLWGHRQYDPIYEAAQEADLPVLMHSVTVIHPVFPFNT
                     QGFETEFGRHICSHTFSMMANIVHMMTTGVPVRYPDLRVAVTEAGISWVPFLMHKMDK
                     EYIERRREVPFLEDKPSTYLKKMYFATQPVEEPENLSDLVTMMELYDGEDNTIFASDW
                     PHHDFDHPSKVHQIPFTSEVRRKVFGENALRLFNIDAGGNRLRVR"
     gene            1877..2239
                     /locus_tag="VN178_10345"
     CDS             1877..2239
                     /locus_tag="VN178_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWS81412.1"
                     /translation="MSREHVIGDLSDFPPGAHRVVKVGRREIGVFNIGGKLYGLPNVC
                     PHQTGPLCEGKPPLGTLVARSENDWKFEWVDEGEVVVCPWHGLEYHVPTGRCLAYPNI
                     TLRSYEVFVEDGEVKVRL"
     gene            2293..2484
                     /locus_tag="VN178_10350"
     CDS             2293..2484
                     /locus_tag="VN178_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81413.1"
                     /translation="MAFLVVREEDPDDWLARFEKDGDFPAREWAENMADVYNRRLDRG
                     STGPPTPAGTRPASYHPEP"
     gene            2546..5344
                     /locus_tag="VN178_10355"
     CDS             2546..5344
                     /locus_tag="VN178_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HWS81414.1"
                     /translation="MKERVWDLREVGRLPAPGDNVAMATRRLEAGTRVSHEGSEFTIG
                     YTVLEGHRFAVERIAEGEDLLSWGLRFGRATRDIAPGDYACNEKILRVLRERLGVSPE
                     REEEDPEGTSDQGGGRVPGGQDEAGLRLPEEPNFSDAELEPYVLDEEGFRPGVQVPLH
                     DEPRTFMGYRRGAGRGVGTRNYIVVMGLTSRLTGFVRALELEMEGVADAYENVEGIVC
                     VAHTEGGEERSPNNLDLLLRTLSGFMVNPNVGAVLVLDHGGEEAVTNSMLEAYLEANG
                     YPIDDVPHEFMSLEGSFRRDLEVAKSVVRGWLEEVDAARRTEEPASELKIGLQCGGSD
                     AFSGVSANPLVALVSGEVVKNGGIANLAETDELIGAEHYVLKNVRDLQTARRFLSTVE
                     RFKERVSWHGHTAEDNPSGGNNYRGLYNISIKSIGAAMKKHEDVRIDRVIEYAQRMDE
                     GGFYFMDSPGNDLESVAGQVASGANMIFFTTGNGSITNFPFVPTIKFVTTTGRYELLS
                     KDMDVNAGAYLDGAPMDELGRETFERTLRAASGERTVGERAGHSQVSIWRDWKQTSDA
                     NLERLRNAPEPGGDPLPVKESAPEVEFSFEALRSRRGPVSDQVGLIMPTSLCSGQISR
                     RIANRLNERGATQGKVTRFVALPHTEGCGVSAGSAEAIYSRTVLGHLASPTVRFGLLL
                     EHGCEKTHNDYFRNCLIEAGLDPGRFGWASVQLDGGIDSVVAKVEDWFEDTLDDAEAL
                     EYVDAGPEALRLGLYAAGPVSDEVSLSLAEITLAVANSGGTVVVPERAAVLSSPAYLE
                     AVLGDLSVENTLAYGQAVPPGKQGLHVMEAPTDHWVETATGLGATGVEVMLAHVAGHP
                     LQAHRMIPLVQASSDPETLKRYADDLDVILQKDATEWTEQMLEAVAAVASREYVPKLF
                     EAGNTDFQFTRGLLGISM"
     gene            complement(5348..>5638)
                     /locus_tag="VN178_10360"
     CDS             complement(5348..>5638)
                     /locus_tag="VN178_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS81415.1"
                     /translation="YSASKAGIANLTRALANEWAPLGINVNAIAPSYFTTELTSALRE
                     DEERSVALLSRLPAGRFGEPEDLKGAAVFLASHAADYVHGTILPVDGGWLAR"
BASE COUNT         1086 a         1736 c         1876 g          940 t
ORIGIN      
        1 cgccatcgtg acgggcgggg gtgggggcct cgccgagggc atctgcgcga ggctcgcctc
       61 ggacggcgcg gccatcgccg cggtcgacgt ctcgcgcgag aaggcggaga gggtcgcgga
      121 ccaggtcagg gcgaatggca ccagcgccgt ggccgtggag gccgacgtct ccgaccgccg
      181 gtcggtccaa gccatggtcg acgaagtcgt gagcgggctt ggcggggtgg acatcctcgt
      241 caacaacgcc gccgtctacc cacggcgcgc ctggacggag atcgaagaag aggagtggga
      301 cagggtgatg gccgtcaacc tcaaaggcta cttcctgtgc gcgcgggccg tctatcctca
      361 tatgaaggag cggggacggg gtagcatcgt gaacgttgcc tcgatcacct ttttcatcgg
      421 gtggacgctc ttgctcgact acgttgcttc gaagggtggc atcgtcggct ttacgcgcac
      481 gctcgcccgc gaggtcggcc cggacaacat caacgtgaac gccatagcgc caggcgcatt
      541 ccccacggac gccgagaaga tccaccccga ccaggaaggc tacaaccgct gggtgctcga
      601 gcaacagagc atcaagcgcc ggggcacacc cgaagacatc ggcaacctcg tcgcgtttct
      661 cgcctccgac tcatcctcct tcataacggg ccagaccatc ggcatcgacg gtggctggat
      721 gatgcgttag gggagggacc gtggccgaag ccgcaacggc aaagagggaa cgcaaagaga
      781 agctcgagga catcctcgtc gtggactgcg acgtacacgt ccacgagtca ccggaggcgc
      841 ttatacccta ctgcgagatg ccctggcgga aggcgctcga gacgataaag gacgtaaagg
      901 agttctacct ggacatcccg ggcttctcgc cggggacgac cgtctacgaa gcgaagttcc
      961 cttcggggca ggaggccacc cgcatggtcc attccgcctc ccagatgcgc gaggagctcg
     1021 acgagatcca cgtggacatc ggtgtcctgt tccccgacca cttcctgaag ataccggtga
     1081 tctcacaggt ggactacgct tcggcgctcg cccgagccta caacgcctgg ctccttgcgg
     1141 agtggtgctc gcccgagaac ggcctgctcg gctgcatcct cgcctgcccg caagaccccg
     1201 tcggcgcggc ccatgagata gagaagtact cggatgagaa gggcatggtg ggggtgttcc
     1261 tgccctgtgc cgggctggag cggctctggg ggcacaggca gtacgaccca atctacgagg
     1321 ccgcccagga ggccgacctg cccgtactca tgcacagtgt gaccgtaatc catcccgtct
     1381 tccctttcaa cacccagggc ttcgagacgg agttcggacg tcacatctgt agccacactt
     1441 tctcgatgat ggccaacata gtgcacatga tgaccacggg cgtaccggta aggtatcccg
     1501 acctccgcgt cgccgtcacc gaggcgggca tctcctgggt gcccttcctc atgcacaaga
     1561 tggacaagga atacatcgaa cgccgccgcg aggtcccctt cctcgaagac aaaccgagta
     1621 cctacctgaa gaagatgtac ttcgccaccc aacccgtcga agagcccgag aacctctctg
     1681 acctggtaac tatgatggag ctctacgatg gagaagacaa caccatcttc gcctccgact
     1741 ggcctcacca cgacttcgac catccctcga aggtccacca gatccccttt acgagcgagg
     1801 tcaggcgcaa ggtcttcggc gagaacgccc tgagactctt caatatcgac gccggcggca
     1861 accgcctcag agtgcgatga gtagggagca cgtcataggc gacctctcgg atttcccgcc
     1921 cggtgctcac agggtcgtga aggtcgggag gcgggagatc ggggtcttca acatcggggg
     1981 caagctctac ggccttccca acgtgtgccc gcaccagacg ggaccgctgt gcgagggcaa
     2041 gccgcccctg ggtaccctgg tcgcaaggag cgagaacgac tggaagttcg agtgggtaga
     2101 cgagggagag gtcgtggtct gcccctggca cggtctcgaa taccacgtgc cgaccggaag
     2161 atgcctcgct tatccgaaca tcaccctccg ctcctacgaa gtattcgtgg aagatggcga
     2221 ggtcaaggtc aggctctagt gtcggaccag ggtcttcggg atggcggcag gctctccgta
     2281 cgcgaggagg ggatggcttt cctggtagtc cgggaggagg accctgacga ctggctggcg
     2341 cgtttcgaga aagacggaga cttccccgcc cgcgagtggg ccgagaatat ggccgacgtc
     2401 tacaaccggc gcctcgaccg gggctccacc ggacctccga caccggcggg tacgcgccct
     2461 gccagttacc atcccgagcc gtagggcacg ttgcgataca ctcgtcgcga gaacgcacgt
     2521 ctgaaaggat aagggaggca ctgctgtgaa ggaacgagtt tgggatctcc gggaagtcgg
     2581 acgcctcccc gcgccgggag acaacgtggc catggcgacg cgcagactcg aggcggggac
     2641 gcgagtgtcc cacgaaggct cggagtttac gatcgggtat acggtgctcg aaggccaccg
     2701 tttcgccgtc gaacgcattg cagaagggga ggatctcctt tcctggggcc tccgcttcgg
     2761 acgcgccacc agggacattg cgcccggtga ctacgcgtgc aacgagaaga tattgcgcgt
     2821 cttgcgcgaa cggctcgggg tctcgcccga acgcgaggaa gaggatcccg agggcacctc
     2881 tgatcagggc ggaggacgtg ttcccggagg gcaggacgag gccggcctga gactccccga
     2941 agagcctaac ttcagcgacg cggagctcga gccctacgtc ctcgacgagg agggcttccg
     3001 ccccggcgtg caggtccccc tccacgacga gccgcgtacg tttatgggct acagacgcgg
     3061 tgcaggcagg ggcgtcggga cgcgcaacta catcgtcgtg atgggtctca cgtcgaggct
     3121 caccgggttc gtcagggcgc tggagctgga gatggagggc gtcgccgacg cctacgagaa
     3181 cgtagaaggc atagtctgtg tggcccacac ggaaggtggg gaggagcgct cgccgaataa
     3241 cctggacctg ctgctgagga ccctgagcgg cttcatggtc aacccgaacg tcggggccgt
     3301 gctcgtgctc gaccacggcg gtgaagaggc cgttacgaac agtatgctcg aagcgtatct
     3361 ggaagcgaac ggctacccaa ttgacgacgt gccgcacgag ttcatgagcc tggaaggtag
     3421 cttcaggcgg gatctcgaag tggccaaatc cgtggtgcgg ggttggctcg aggaggtcga
     3481 tgcggcgcgg cgcaccgaag agccggcttc cgagctcaag ataggactcc agtgtggggg
     3541 atcggacgcc ttctcggggg tctccgcgaa cccgctcgtg gcgttggtga gcggggaggt
     3601 ggtcaagaac ggtggtatcg ccaacctggc cgagacggac gagttgatcg gggccgagca
     3661 ctacgtcttg aagaacgtca gagacctgca gaccgcgcgg cgcttcctct ctacggtcga
     3721 gcggttcaag gagcgcgtct cctggcacgg tcacaccgcc gaagacaatc cgtctggggg
     3781 caacaactat cgtgggctct acaacatctc catcaagtcc ataggggccg cgatgaagaa
     3841 gcatgaagac gtgcggatcg accgtgtgat cgagtacgcc cagaggatgg acgaaggcgg
     3901 cttctacttc atggacagtc ctggcaacga cctggagagc gtggcgggac aggtcgcctc
     3961 tggtgcgaac atgatcttct tcaccacggg caacgggtcc ataacgaact tccccttcgt
     4021 cccgaccata aagttcgtca ccaccacagg ccgctacgag ctcctctcca aggacatgga
     4081 cgtgaacgcc ggcgcctacc tggacggcgc cccgatggac gagcttggcc gcgagacgtt
     4141 cgagcggacg ctcagggccg cctcgggcga gaggaccgtc ggagaacgtg ccggccactc
     4201 ccaggtctct atctggcgcg actggaagca gacgagcgat gcgaacctgg agcggcttcg
     4261 gaacgcgccg gagcccggcg gggatccatt gcccgtaaag gagagcgctc ccgaggtcga
     4321 gttctccttc gaggccttaa ggagtcggcg tgggccggtc tcggatcagg tcggtctcat
     4381 catgccaacg agcctttgct cgggtcagat ctcacgccgc atagcgaacc gtctgaacga
     4441 acgcggcgcc acgcagggca aagtaacccg cttcgtcgcc ctgccccaca cggagggctg
     4501 cggcgtctcc gccggctcgg ccgaggctat ctactctcgc accgtgctcg gccacctcgc
     4561 cagccctact gtcagattcg gccttcttct ggagcacggt tgcgagaaga cccacaacga
     4621 ctacttcagg aactgcctca tagaggcggg cctggatcca ggccgattcg ggtgggccag
     4681 cgtccagctc gacggcggca tagatagcgt ggtcgcgaaa gtcgaagact ggttcgagga
     4741 taccctggac gacgcagaag ccctggagta cgtggacgct ggccccgaag ccctgcgcct
     4801 tggtctgtac gccgccggac ccgtttcgga cgaggtttct ctcagcctcg ctgagataac
     4861 gctcgccgtc gcgaactccg gcggtacggt ggtcgtcccc gagagggccg cggttctctc
     4921 ttcgccggct tatctggagg ccgtactggg cgatctctct gtggagaaca ccctcgctta
     4981 tgggcaggcg gtgccccctg gcaagcaggg actacacgtc atggaggcgc cgacggacca
     5041 ttgggtggag acagcgaccg ggttgggggc gaccggagtc gaggtcatgc tcgcgcacgt
     5101 ggcggggcac cctttgcagg cgcaccgtat gatcccgctg gtgcaggcct cgtccgatcc
     5161 cgagacgttg aagagatacg cggatgacct cgacgtgatc ctgcaaaagg acgcaaccga
     5221 atggaccgaa cagatgctcg aggcggtcgc ggccgtggcc tcgcgagagt atgtccccaa
     5281 actgttcgag gccggcaaca ccgacttcca gttcacccga ggtctgctcg ggatctcgat
     5341 gtagtctcta gcgtgccagc cagccgccgt ctacgggcag gatcgtaccg tgaacgtaat
     5401 ccgcggcgtg ggaggcgagg aagacggcgg cgcccttgag gtcctctggt tcgccgaagc
     5461 gtccggcggg gaggcgggaa aggagcgcta cgctacgctc ctcgtcctcg cggagcgcgc
     5521 tggtcagctc ggtggtgaag tagctggggg cgatggcgtt gacgttgatc cccaggggcg
     5581 cccactcgtt ggcgagggca cgggtaaggt tggcgatgcc cgccttggat gcggagta
//
LOCUS       DATMVF010000208        17437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_172789, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000208 DATMVF010000000
VERSION     DATMVF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 17437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17437
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172789"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..745)
                     /locus_tag="VN178_10365"
     CDS             complement(2..745)
                     /locus_tag="VN178_10365"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWS81416.1"
                     /translation="MGVLQAKASGGSREHENTPECSAESGPSGALRWSAAGKLLVIVV
                     LAVGLPMLVGALGGGERLRVLVEQSGPWAPLTYVAAKAASTIIAPFSGVPLKAASGAL
                     FGLTGGVFYSLFGDVIGGCVCFLAARYVGWSTVERFTGDGTMARLNRVIDRGLGGWRE
                     LLFLRVTFPAIYNLLSYAAGSTRLPFRQYLAVTAFGGIIHTSILVTLGASVAVGWEGR
                     VAAFAGIALLSLLALFGVRHLRSVLTRGT"
     gene            822..1607
                     /locus_tag="VN178_10370"
     CDS             822..1607
                     /locus_tag="VN178_10370"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWS81417.1"
                     /translation="MKRPLRNEWAARLWSGPVVVGLLALVALAVIGFFVVRAFGGSEQ
                     MAGLMEQLQTAGLRMRDWIEQSGPLAPLTYLVAKAATFIFLPWAGYPLNVASGALFGL
                     FWGVILTALGDTLGGCILYGLSRWAGRPAVARILGESRMARVDRVVDTGLGGWRELLF
                     FRVVVPIPYNLVSLAAGLAPTLTLRQYATVTFLTAAPKVFTVGIGAGLVTGEWMEVAV
                     AGGLVVIAVAAMLTVRSVREALMRALRWRRREKRIEDGTEQGT"
     gene            complement(1662..2717)
                     /locus_tag="VN178_10375"
     CDS             complement(1662..2717)
                     /locus_tag="VN178_10375"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HWS81418.1"
                     /translation="MIEIGVIGGDGIGPEVVRAGMQVLEAAAKDDPSLELAFTEFPWG
                     CEYYLEHGRMMPEDALDTLAACDEIYLGAVGWPTVPDHLSLWGLLLPIRRTFDQYVNL
                     RPARLLRGVKSPLAEPNDLDITVVRENTEGEYSDSGGRVYKGTPHEIAVQESIFTRRG
                     VERIIRFAFEEAKARRGLLVGATKSNGISVTMPFFDEIFREISSDYPGVEASLMHADA
                     LAARLVLAPHSFDVVVGSNLFGDLLSEVTAAIAGAIGIAPSANLNPEGTYPSLFEPIH
                     GSAPDIAGQGLANPSGAIWAGAMMLDHAGFPEAGARILAALEETLASGTKTRDLGGTA
                     STDEMADAVAQRLGKSA"
     gene            2853..3188
                     /locus_tag="VN178_10380"
     CDS             2853..3188
                     /locus_tag="VN178_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81419.1"
                     /translation="MDERDARRNREASERLDRLAREGYKAAVDQAFEAQKSGMRLSRR
                     FFENWVETLDDQAELNRRTLGSLQQLAREQSEVFRALSRESVDAYDGFLDSLDAYQQD
                     ISKLQERDD"
     gene            3283..4674
                     /locus_tag="VN178_10385"
     CDS             3283..4674
                     /locus_tag="VN178_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HWS81420.1"
                     /translation="MREEELPESLSGGPTSGGFEGVNPLATDVLRRNGLKSWTKPAPS
                     LYPHQWSWDSAFIALGLAHVDNRRATDELETLFSGQWSTGKVPHIVFDPEAPKESYFP
                     DAERWNSSALSEDAPSGPRTSGLCQPPVHAIAVRRIWQTTRGKDEERVDRARSFLSGN
                     YRRLFAWHRYLATARDPEGSGLVTIYHPWESGTDNSPRWDAALERIPVGEVPSYTRYD
                     LKHVSDPSHRPTDSEYDRFLWLLELLKRVRYDEADIYQRHPFLVKDVLFSGILVAANE
                     ALLEISEVVEAPREDRALIEGWIERGRAGLEEQWDPHLGLCLDQDVIAGEPLRVRTVA
                     GFAPLIAGGLDRSRLRDLLGTLDSKAFAGDPRLRHPLPPSTSPEEPGFHPRSYWRGPV
                     WPVANWLFWWSLLRAGESEKAQRIRRETLEELSEGGFAEYFEPFTGEALGSGEQSWTA
                     AVALDMLATGGAD"
     gene            4835..5101
                     /locus_tag="VN178_10390"
     CDS             4835..5101
                     /locus_tag="VN178_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81421.1"
                     /translation="MRVAPPEDAGSEVAEFRPHLLVHNDTDGFGPEDVASVPCRVTVL
                     YSDGMDARISADGQFSRTSDMSTEDLLQVVDVAIALAERETGEA"
     gene            5561..6268
                     /locus_tag="VN178_10395"
     CDS             5561..6268
                     /locus_tag="VN178_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81422.1"
                     /translation="MGEADTIRDVWLGALALFRRYPLATLAPAAVLGAIGEVPAYLID
                     GRPLLDQVLTLVSSYAAYYLYLAYAEGIVSRAQRGIQGPGLRSMLDDLMEAAPFVPSV
                     LVAALISLFVTTLAIGLLVIPGMWLYTRWSLATPVIREQSVGPLAAIRRSNQLVRGHF
                     WFVFMTATVAYYLEGVVVHEGAVVAGSVTGSHTWGAWAGGSIVATLVVPLAAFATSLA
                     HSSVRRRAYQGDKTGTV"
     gene            6407..6916
                     /locus_tag="VN178_10400"
     CDS             6407..6916
                     /locus_tag="VN178_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HWS81423.1"
                     /translation="MLGEAVEDAMNEQIKNELFSAYQYLSMAAYCESENLPGFAQWMR
                     AQAREETAHAMKFYDFILDRNGRVVLRGIDEPVVGFGSPLEVFEQALEHEKKVTTMIH
                     DLYGLAVKENDYASQTFLQWFVTEQVEEEKNAGDVVETLKMVGDKSEALFLLDRELGQ
                     RRAAEEPAG"
     gene            6971..7399
                     /locus_tag="VN178_10405"
     CDS             6971..7399
                     /locus_tag="VN178_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWS81424.1"
                     /translation="MAQQMTDEEYRSFMLDRARTAVLATVRADGRPHTAPIWFDLDGD
                     TLVFTTGESTVKGRNISRDPRVSLCVEEEEPPFHFVVIEGSSEPTAGDPDLLYWATRI
                     GGRYMGADRADEYGRRNAVEGELLVRVTPHKVVAYKNISD"
     gene            7563..8258
                     /locus_tag="VN178_10410"
     CDS             7563..8258
                     /locus_tag="VN178_10410"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWS81425.1"
                     /translation="MNERQEDKGENHGLNDLAHRLREAVSRPLDEWREVYEGFSPVDL
                     ESGERRPRVPPPGTDTRRAAVLVPIIVEGEDARVVYTVRKGDLADHAGQISFPGGSME
                     ARDASLLETALREAEEEIDLRPELVEVVGELEEMYIPPSNFRVSPFVGLLPPGAEMVL
                     APDEVEEIFTVPLKTLSASETYRKVLWRREGRDYKVPVFAIEGAVPRNIWGATAAMTA
                     ALLARLGWREYRR"
     gene            8263..8664
                     /locus_tag="VN178_10415"
     CDS             8263..8664
                     /locus_tag="VN178_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81426.1"
                     /translation="MLRLVAMVLFGLMFLAEAGDIYGLVLTLADPGPTADRFGITAGA
                     EVLRSTVLLMLALVVFFGALLALVGLFARKPELFHTSALVCAIGYLVYGLIQVADGAL
                     QLGSAIVVVAGLIYVVLGGLAYAMYRTAFKL"
     gene            8881..10128
                     /locus_tag="VN178_10420"
     CDS             8881..10128
                     /locus_tag="VN178_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWS81427.1"
                     /translation="MAEWRGVYARHRLDISVGDMFFGVLSCGLRLRREKLEAGILRLC
                     SLEEEGLVSFSVRSGWDLWLRARGLRAGDEVLVSAITHPDMVRIIHEHGLRAVPVDID
                     PGSLAVRPWMLEAALTPRTRVVLVAHLFGGRMDLGGVAEFARDRGLLLVEDCAQAFRG
                     PKRMGDGAADVSMYSFGTLKTSTALGGAVLRVRDREVLERMRGIQASYPPQGRGGYSK
                     KLLGVFGLVAVSRPRPYGLLARGCARLGYDLDTLVGGLVRGFPPHETAATFFRRLRHR
                     PCAPLLAMLYRRMRTFDGRRLARRAKTGERFASRLRVLDLQPGRHSPLRTHWLFPVVV
                     ADPGALVADLRRHGLDASRATSSIAAVEAPPGRSSPAEASLMMSGVVFLPVYPELPSQ
                     AFDVMASLVNDCAARGTAEQVAL"
     gene            10173..11660
                     /locus_tag="VN178_10425"
     CDS             10173..11660
                     /locus_tag="VN178_10425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWS81428.1"
                     /translation="MFDLLVIGGGIVGARVAFDAARAGLRVALVDAGDFGGATSGASA
                     RLVHGGLRYLGTGDFRLVRMALRERSVLASRVAPHLITPLPFVLCAAEGRRSRCAAGL
                     LVYAALDGFRSPLPRFVTPEEASFLVPSLCVGESASHAVYYEAATNDSRLALATVTAA
                     ARGGAVVANYLRAVDLAPGRISRVSLEGRRGEVTVRCRAVVNATGPWLDLLRNMEDPA
                     CEPITRLSKGIHVVLRPDQQWRGAAAVSLHDGQHLYAVPCEDRILLGTTEQEYQGDPT
                     AVTAEPGDVSYLLQVANQFLRSEMLRRERVASAFAGLRVLPRGEEATLHASRGHLLSV
                     GPGGMVSVAGGKLTTHRRIALDALRHLPDRVRPRRLYLSDAPLPGASPAPVHHSHLDG
                     STLDHLVHLYGSETGTLLGQCKVHYNALERITAGAPDLWAQVYHAVREEWALSAEDII
                     YRRTSLGLRGFDTPELRQRISAVLEAEDPGLFEPNLRESTHLSSS"
     gene            11991..13475
                     /locus_tag="VN178_10430"
     CDS             11991..13475
                     /locus_tag="VN178_10430"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HWS81429.1"
                     /translation="MSRKFTRQKFLGVAGTGAAYIALLSLPGCRKEQQSHSSPPAQPD
                     NVRTFRSRPDLSPPAIEIHERARGSAPGYIFIAAKKGAGQDGPMIVDDLGRPVWFSKD
                     RYATDFKVQRYRGEPVLTWWQGEIVAGHGEGEYVIFDDSYREITRVQAGNGYRGDLHE
                     FSITQQDTALLTLYVQTQADLSPIGGPKDGMVWEGIAQELDLETEEVLFEWRSLEHVG
                     VEESYREPPQEPTTPLDYFHINSIDIDFDGNWLISAKGTSAVYKVDRQSGEILWRLGG
                     KESDFQMGEGTRTVSQHDARRQQDGTITIFDNGAPPQVHEQSRTIAVSLDMDEMSAAL
                     VREYTHPQKLIATSQGNAQVLPNGNVFVGWGSSPFFSEYTKDGKLHFDAEFPGSAQSY
                     RAFRFPWKGDPVEEPAVAVDTGPDDRVTLFASWNGATEVESWEALAGPGPDQLEPVGT
                     SPRHGFETTLTMRTAGPYVAVRAKSAAGQVLGTTRAIKLGAQTS"
     gene            13678..15291
                     /locus_tag="VN178_10435"
     CDS             13678..15291
                     /locus_tag="VN178_10435"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HWS81430.1"
                     /translation="MGERTRLGLGVLGVALVLGGLGDLLLRATPWGINLLIWVTAIVG
                     LAILLAKWGRVGTGGEGKWLVFVALVFAAGLVLRDSPVVVFLDLLGILISLSLAVWLG
                     RNGSLRRAGVLDYILGGVYTGVLTSAGPVPVSVADIEWREAVRGRWKGQVLAVSRGVL
                     LAAPLLLLFGALLVAADAIFERLIIEVFGFDLAEVFSHLALITFFAWISAGVLWAGLM
                     ARVPENFSIPRLRVLSLGIIEVGIVLGLLDLLFLAFVVIQVRYLFGGAGRVAATAGLT
                     YAEYARRGFFELVTVTALALPLLLVAHWLLRSGTRAHERLFKALAGIMVALLFVVVAS
                     ALQRMYLYQQEFGLTELRLYTTIFMVWISVVLLWFVITVLRARRDRFAFGALLAGFAA
                     IFAINAMNPDALIASTNIDRMEAGKRFDAYYLTTLSADAVPVLVESLPQIGDDRLWKA
                     YTVEQAVVDRWYSRDADWRTWNLGRSRARHLVESYVEDQDLQASRVIRPDSGHQDRAF
                     VHRPDRVIHNMLWIRSPQRGITGSTVTSS"
     gene            15440..15742
                     /locus_tag="VN178_10440"
     CDS             15440..15742
                     /locus_tag="VN178_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HWS81431.1"
                     /translation="MITEERVQDQLRNVIDPEIGMDLVELGLIYDVGVHDEGRHVDVT
                     FSLTSPMCPVGDLIQEQVETETLSIEGVETVNAQLTFEPMWSPEMMSPAAKLFFGR"
     gene            complement(15803..15937)
                     /locus_tag="VN178_10445"
     CDS             complement(15803..15937)
                     /locus_tag="VN178_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81432.1"
                     /translation="MRRLEQVTVAFGEPIDPSGFHEYARPREQVVEAIRERVAELGGR
                     "
     gene            complement(15985..16725)
                     /locus_tag="VN178_10450"
                     /pseudo
     CDS             complement(15985..16725)
                     /locus_tag="VN178_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
     gene            complement(16986..>17437)
                     /locus_tag="VN178_10455"
     CDS             complement(16986..>17437)
                     /locus_tag="VN178_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81433.1"
                     /translation="MEQGQAPLMTQTRGASSGWTIIDDIRQNLQNFEPRTKAEEQLYA
                     EGLDQVDTLADARRTRLVAAEEGVPGVMWSVLVFGGIAAIGFTYLFGLESTWAHRLMV
                     GTLAAVIGLVLFTIGAMEHPFSGGARIGTGAFELVLERFETSKLSDL"
BASE COUNT         3109 a         5404 c         5667 g         3257 t
ORIGIN      
        1 ctcacgtccc gcgggtgaga acgctccgca ggtgccgcac tccgaacaat gccaacaagg
       61 acaacagcgc gatcccggcg aacgccgcca cgcgcccttc ccagccgacg gcgacgctcg
      121 ccccaagggt gacaagaata ctggtgtgga tgatgccgcc aaaggcggtg acggccagat
      181 actgccggaa gggcagcctg gtcgagcccg cggcgtagct cagcaggttg tatatggcag
      241 ggaacgtaac cctgaggaag agcagctcgc gccagccgcc gaggccgcgg tcgatgaccc
      301 ggttcagacg ggccatggtg ccatcccccg tgaaacgctc cacggtgctc catcccacgt
      361 agcgtgcggc cagaaagcac acgcagccac cgatgacgtc gccgaacagc gagtagaaga
      421 cgccgcctgt gagcccgaag agcgcgccag acgccgcttt cagcggcacg ccgctgaacg
      481 gtgcgatgat cgtcgacgca gccttcgcag cgacgtaggt aaggggcgcc cagggacctg
      541 actgctcgac gagcacccgc agcctctcgc cgccgccgag cgccccgacc agcatcggca
      601 accccaccgc aagaaccacg atcaccagga gcttgccggc agcactccaa cgcaaagcac
      661 ccgatggccc tgattctgcc gagcactccg gggtattctc gtgctctctg gagccccccg
      721 atgctttcgc ttgcaaaact cccaaagaac ccctatcgtt taagatctga tattgagact
      781 catttgcaca accaggatac acgagaagaa agtgaggggc aatgaagcgt cctttgcgga
      841 atgagtgggc ggccagactg tggagcggtc cggtcgtcgt tggcctgctc gcgctggtgg
      901 cgctggcggt gatcgggttc tttgtcgtaa gggcgttcgg tggcagcgag cagatggctg
      961 gcctaatgga gcagcttcag acggcggggt tgaggatgcg ggattggatc gagcagtccg
     1021 gccccttggc ccctctgacg tacctcgtgg cgaaagccgc aaccttcatc tttctgccgt
     1081 gggccggata cccgctcaac gtggcttctg gtgcgctctt cgggctgttt tggggcgtca
     1141 tactgacggc gctcggagat acgttgggcg gctgcattct gtatggcctg tcaagatggg
     1201 caggccgtcc cgccgtggcg cggatcctgg gtgagagtcg catggcccgg gtggacaggg
     1261 tcgtagacac cggcctcggc ggctggcgtg agttgctctt cttcagggta gtggtgccga
     1321 taccgtacaa tctggtcagc ctcgccgccg gcctggctcc cacgctgacc ctacgccagt
     1381 acgcgaccgt tacctttctg acggccgctc ccaaagtctt cacggtgggg atcggtgccg
     1441 gcctggttac gggtgagtgg atggaggtcg ccgtcgcggg cgggctcgtc gtgatcgccg
     1501 ttgcggcgat gctcacggtg cggagcgtca gggaggcctt gatgcgcgct ctgcggtgga
     1561 ggcggaggga gaagcgcata gaggatggta cagaacaggg cacgtgagga cgatagtggg
     1621 ctgacgctgc ccggctatcg agagtaggaa gcaggcctgg ttcaggcgga ttttccgagt
     1681 ctctgcgcga ctgcgtcggc catctcgtcc gtactcgcag tgccgccgag gtcgcgggtt
     1741 ttcgtgcctg atgccagggt ctcttcgagg gcggcgagga tacgggcgcc tgcttccggg
     1801 aatcctgcgt ggtcgagcat catggcaccg gcccagatcg cgcccgatgg gtttgcgaga
     1861 ccctggcccg cgatatctgg agccgagccg tggatgggtt cgaagaggga ggggtacgtt
     1921 ccttccgggt tcaggttggc cgagggagcg atcccgatgg ccccggcgat ggcggccgtg
     1981 acctccgaga gaaggtctcc gaagaggttg gagccgacga cgacgtcgaa ggagtgcggg
     2041 gcgagcacga gcctggcggc cagggcgtcg gcgtgcatca gggaggcctc gactccagga
     2101 tagtcagagg atatctcgcg gaagatctca tcgaagaaag gcatcgttac gctgatgccg
     2161 ttggacttgg tagccccgac gaggaggcca cgtctggcct tcgcctcctc gaaagcgaaa
     2221 cggatgatcc tctccacgcc gcgccgggta aagatgctct cctggacggc gatctcatgg
     2281 ggtgtacctt tgtaaacccg cccgccggag tctgagtatt cgccttccgt attctccctg
     2341 acgaccgtga tatcgaggtc gttcggctcc gccagcgggc tcttgacgcc gcgcaagagc
     2401 cgcgcggggc gcaagtttac gtactggtcg aacgtgcgcc ttatggggag caggagcccc
     2461 cagagggaga ggtgatccgg caccgtcggc cagccgacgg cgccgaggta gatctcatcg
     2521 caagcagcaa gggtatcgag agcgtcttcg ggcatcatgc gcccgtgctc gagatagtac
     2581 tcgcagcccc aagggaactc cgtaaaggcg agctcgaggg agggatcgtc cttcgccgcg
     2641 gcctcgagta cctgcattcc ggcccgcacg acctcgggac ctatgccatc accgcctatg
     2701 accccgatct cgatcacggc cccatgctag caaagaggtc gccatcgagc acgccagaga
     2761 tataatccta tcgacgttgg gagaggcggt gcccacggct aatctttgga tcctgcacgg
     2821 atcggcgggt atcacgggac aggaggtctg cagtggacga gagagacgcg cggcgcaaca
     2881 gggaagcttc ggagagactc gacagactcg cccgcgaggg gtacaaggca gccgtggacc
     2941 aggctttcga agcgcaaaag agcggcatga ggctctccag gaggtttttc gagaactggg
     3001 tcgaaaccct cgacgaccag gccgagctca accgcaggac cctgggtagc ctgcagcagc
     3061 tcgcgaggga acagagcgag gtcttcagag cattatcccg ggagtccgtc gacgcctacg
     3121 acggcttttt ggattccctg gacgcctacc agcaagacat ctctaagctg caagagcgcg
     3181 acgattgagc ggggatcttg cgggtgggct tgaaggcagg ggcctggcct tgtatacatt
     3241 gaccgggtag acctagtcga cgggagttac cagcaccggt tcatgcgaga ggaagagctt
     3301 ccagagagcc tttcgggagg gccaacgtcg ggcggcttcg aaggagtcaa cccactcgcg
     3361 acggacgtct tgcgccgcaa cggcctgaaa tcctggacaa aaccagcacc ttctctgtat
     3421 ccgcaccagt ggagttggga ctccgcgttc atagcgctcg gtctcgcgca cgtggacaac
     3481 cgccgcgcga cggacgagct cgagaccctg tttagcggcc agtggtcgac gggcaaggtt
     3541 ccccacatag tcttcgaccc ggaggcccca aaggagagct acttccccga tgcggagcgc
     3601 tggaattcct ccgcgctctc cgaggatgcg ccctcgggcc cgcgcaccag cgggttgtgc
     3661 cagccgccgg tccacgccat cgccgtcagg cgtatctggc agactacgcg gggtaaggat
     3721 gaagagcgcg tcgaccgggc ccgctccttc ctcagcggta actaccggcg cctgttcgcc
     3781 tggcaccgct acctggctac cgcccgtgac ccggaaggtt cggggctcgt gaccatctac
     3841 catccctggg agagcggcac ggataactcc ccgcgctggg acgcagccct ggagaggatc
     3901 cccgtcgggg aggtgccttc ctacacccgc tacgatctca agcacgtcag cgatccctcg
     3961 caccgtccga ccgacagcga gtacgaccgc tttttgtggc tactggagct tctcaagcgg
     4021 gtccgctacg acgaggccga catctatcag cgacacccgt tcctggtcaa agacgttctg
     4081 ttttcgggca tcctcgttgc ggccaacgag gcgttgttgg agatctccga ggtcgtcgaa
     4141 gcaccgcggg aggacagggc gcttatagag ggctggatcg aacgcggccg tgcagggctc
     4201 gaagagcagt gggaccccca cctggggctt tgcctggacc aggacgtaat cgcgggcgag
     4261 cccctgcggg tgcgcaccgt ggcaggattc gcgccgctga tcgccggcgg actagaccgc
     4321 tcacggctcc gcgatctcct cgggacgctc gactctaagg ctttcgctgg agaccccagg
     4381 ctgcgccatc cgctgccgcc ttccaccagc cccgaagagc ctggtttcca cccgcgcagc
     4441 tactggcgtg gtcccgtatg gcccgtagcc aactggctat tctggtggtc gctgcttcgc
     4501 gcgggagaga gtgagaaggc ccaaaggatt cgccgcgaga ccctggagga gctctcagag
     4561 gggggcttcg cagagtactt cgagccgttc accggcgagg cactcggctc cggggagcaa
     4621 tcctggaccg ccgccgtcgc cctcgacatg ctcgccaccg ggggcgcaga ctagggattc
     4681 gtacaccttt cggcccattt tttgtgcgtt tcgttacctg gtgttcctca cggggtatac
     4741 ggtaaggtac gatgcgtgtg ctgatcaccg tcaaccccgt atgtcccgcc aggctatggc
     4801 gctctcgatc cgtcgccagc gtccaggact cgacgtgagg gtcgccccac ctgaggacgc
     4861 agggagtgag gtggcggagt tccggcccca cctgctcgtc cacaacgaca ccgatgggtt
     4921 cggtcctgag gatgtggcga gtgttccatg ccgcgtcacg gtgctctata gcgacggcat
     4981 ggatgcgcgc atcagcgccg acggccagtt ctccaggacg agcgacatgt ccacggaaga
     5041 tctgcttcag gttgtcgacg tggcgatcgc gctagcggag cgggagacag gtgaagccta
     5101 gatccggaca gcggatcagt cttccagagg tactcgtatc tccatcgtag tgccgtcgcc
     5161 gggggtgcga ggtgagattc agttccccac ccaccatctc cgcccgttcc ctcatggacc
     5221 tcagcccgat actcgttcgg tgatcatcgc ccgaggcgtc gcttgtgaca gcatctgcgt
     5281 cgaacccgtc ccctcgtccc tcacggtccc cactaacctg ccggggtgga tctcgaggct
     5341 ccagtccttc gcagcccgag tgcctgaggg cgttcgcgaa cgccttccgc atgatcatgt
     5401 agatttgcac tccgacgtct gccaggaacg agttctcggg ccgtcttgct ttcggcggca
     5461 tctggcgtga gatacttctc ttccgattcg cgcaacgctt cctcggcgcg ggtcggcgag
     5521 tttgccaggt gccgagggct gggcgagaat gcgcggaggt atgggcgagg cagacaccat
     5581 cagggacgtg tggctggggg ccctggcgct cttcaggagg taccccctgg cgacgctagc
     5641 gccggccgcg gtgctcggtg ccatcgggga agtccccgcc tacctcatcg acggtcgacc
     5701 cctgctggac caggtcctca ccctggtgtc ctcctacgca gcttactacc tgtacctggc
     5761 gtacgccgag gggatcgtca gtagggcgca acggggcatt caaggccccg gattacgcag
     5821 catgctcgac gacctgatgg aggccgcgcc cttcgtccca agcgtcctcg tggccgcgct
     5881 gatctcactc ttcgtgacca ccctcgcgat cggcctgctg gtgatcccgg ggatgtggct
     5941 gtacacgcgg tggtcgctgg ccacgccggt gatccgtgag caaagcgtag gtccccttgc
     6001 tgcgatacga cgcagcaatc agctcgtacg cggccacttc tggttcgtgt tcatgacggc
     6061 gaccgtggcc tactacctgg aaggagtggt ggtccacgag ggcgcggtgg tggccgggtc
     6121 ggtcacgggg tcgcacacgt ggggggcatg ggcggggggt tcgatcgtgg cgacactggt
     6181 agtgcccctg gcggccttcg ccacctcgtt ggcccactcg agcgtcagga gacgggcgta
     6241 ccagggcgac aagactggca ctgtatagtc accctttcga gatgagcagc tctgccgctg
     6301 gacatcctgc ggggaggcta gtctacgatt tctacagact gaccgacggg gagatcggta
     6361 tagtgggggc agggacagag cgaacgatga gaaggaggag atcacattgc ttggcgaagc
     6421 ggtagaggac gcgatgaacg agcagatcaa gaacgagctc ttttcggcct accagtatct
     6481 ctcgatggcc gcgtactgcg agtcggagaa ccttcccgga ttcgcgcagt ggatgcgcgc
     6541 ccaggctcgg gaggagacgg cgcacgcgat gaagttctac gatttcatcc tcgaccgcaa
     6601 cggacgcgtc gtcctgcggg ggatagacga gcccgtggtc ggattcggct ccccgctgga
     6661 agtcttcgag caggcgctgg agcacgagaa gaaggtcacg accatgatcc atgacctcta
     6721 cgggcttgcg gtgaaggaga acgactatgc gagccagact ttcctgcaat ggttcgttac
     6781 ggagcaggtc gaggaggaga agaacgcggg tgacgtcgtc gagaccctga agatggtcgg
     6841 tgacaagagc gaggccctct tcctgctcga cagggagctg ggccagcgcc gggcggccga
     6901 ggaaccagcc ggctgacgat ccgattctct ctgtgtctac cttcttcaat agtgtctgtg
     6961 gaggataacc gtggcgcaac agatgacaga cgaagagtac aggagcttca tgctcgaccg
     7021 ggccaggacg gccgttctcg ccaccgtgcg cgccgacgga aggccccata cggccccgat
     7081 ctggttcgac ctggacggcg atacgctggt attcacgacc ggagagagca cggtgaaggg
     7141 acgaaacata agccgcgatc cccgcgtcag cctctgcgtc gaagaggaag aaccaccctt
     7201 tcacttcgtc gtgatcgaag gttcctccga accgaccgcc ggggatcccg acctccttta
     7261 ctgggcgacg cgaatcgggg gccgttacat gggcgccgac agggccgacg agtacggccg
     7321 tcgcaacgcg gtagagggtg agctgttggt gcgcgtaacg ccgcacaagg tcgtcgcgta
     7381 caagaacatc tccgattgac agggctctgg aaaatcaaac cttccagaac cctgtcagca
     7441 tgtcagcaag gactcgcgcc cgcgataagc tgacgagctg aattgctgac aagcgacagc
     7501 cggaggctgg agcgtgctga catgctgaaa tgcttccttg ttggtacgct atgatctcgg
     7561 tgatgaacga acgccaggag gacaagggcg agaaccacgg cctgaacgat ctcgcgcatc
     7621 ggctgcgtga ggcggtatcc cgcccgttgg acgagtggcg cgaggtctac gagggcttct
     7681 cgcccgtgga tctcgagtct ggtgagagac gcccgcgggt gccgccgccc ggcaccgaca
     7741 cccggcgcgc ggccgtgctg gttccgatca tcgtagaggg cgaggatgcg cgggtggtct
     7801 atacggtgag gaaaggagat ctggccgacc acgccggcca gatctccttc cccggcggca
     7861 gcatggaggc ccgcgacgct tccctcctgg agaccgccct acgcgaggcc gaggaggaga
     7921 tcgacctcag acccgaactg gtcgaagtgg taggggagtt ggaagagatg tacattccgc
     7981 cctcgaactt ccgcgtcagt cccttcgtcg ggctcctgcc gcctggggcc gagatggtgc
     8041 tggcaccgga cgaggtcgag gagatcttca ccgtccctct gaagactctg agcgccagcg
     8101 agacgtaccg caaggtgctc tggcgccgcg aaggccgcga ctacaaggtg cccgtgttcg
     8161 ccatcgaggg tgccgtccca cgaaacatct ggggcgcgac ggcggcgatg accgcagccc
     8221 ttttggcacg cctgggctgg cgggaataca ggcgctaggg gaatgctacg attggtcgcc
     8281 atggtgctct tcggccttat gtttctggcc gaagcagggg acatctacgg cctcgtcctt
     8341 acgctcgccg atcctgggcc cacggccgac cggttcggca tcaccgccgg agccgaggtc
     8401 ctgcgcagca ccgtgctcct gatgctggca ctcgtcgttt tcttcggcgc gctcctcgcc
     8461 ctggtcggat tgttcgcccg gaagccggag ctctttcaca cgagcgccct ggtgtgcgcg
     8521 atcggatacc tcgtgtacgg cctcattcag gtggccgacg gggcgctgca actcggctcg
     8581 gccatagtgg tcgtcgcggg gttgatctac gtggtgctcg gcgggctcgc ttacgcgatg
     8641 tatcgcaccg cgttcaagct ataaagcccc tgccctagtg ccagatccca cccccgaacg
     8701 caccacctga tggcgccgca caatcgaaag ccacgcgact gcgctcgtcc ctactaatgc
     8761 cactgaacgt ggcataagat agctttacgg tgtaaactta cgtcgtaaga tagagaatga
     8821 aacgaagagg cgaggcgtta tgggttcgag tgggtgatga ggtgttgggg gacgggtggt
     8881 atggctgagt ggcgtggtgt atacgcgcgg cacaggttgg acatctcggt gggggacatg
     8941 tttttcgggg ttctttcgtg tggcctgcgg ttgagaaggg agaagttgga ggccgggatc
     9001 ctgcggctgt gctccttgga ggaagagggg ctggtcagtt tctcggtgcg gagtgggtgg
     9061 gacttgtggc tccgtgcgcg gggcctacgg gccggggatg aggtcctcgt ttcggccatc
     9121 acgcaccccg acatggtccg cataatacat gagcacggtc tacgggccgt acccgtggac
     9181 atcgatcctg gttcgctcgc ggtgcggccc tggatgctcg aggcggccct gacgcctcgc
     9241 acgcgggttg tcctggtcgc gcacctgttc ggggggcgta tggatcttgg tggcgtagcg
     9301 gagttcgcga gggatcgcgg cctgctcctc gtcgaggact gcgcccaggc gtttcgaggg
     9361 ccgaagagga tgggggacgg ggcggcggac gtctccatgt acagtttcgg caccctcaag
     9421 acctcgaccg cgctcggcgg cgcggtactg cgcgtacgcg acagggaggt gcttgagagg
     9481 atgcgtggga tccaggcctc ctatccgcca cagggcaggg gcggatactc gaagaagctc
     9541 ctcggggtct tcggcctcgt cgccgtctcg cgaccccgcc cttatggttt gctcgcgcga
     9601 ggttgcgccc ggctcggcta cgacctcgac acgctggtgg gcggcttggt ccgcgggttt
     9661 cccccgcatg aaacagctgc gacgttcttc cggcgactga ggcataggcc ctgtgctccg
     9721 ctgctcgcga tgctctacag gaggatgagg actttcgatg ggcgacggct ggcgaggcgg
     9781 gcgaagaccg gcgagcggtt tgcgagcaga ctaagggttc tcgatctgca acccggccgg
     9841 cattcgccgc tgaggacgca ctggctgttc cctgtggtcg ttgcggaccc cggggccctg
     9901 gtggctgacc tccggcggca tggcctggat gcgagccggg ctaccagcag catcgccgcc
     9961 gtcgaggcgc caccgggccg ttcttcgccg gccgaagcat cgctgatgat gtcgggcgtg
    10021 gtgtttctgc cggtgtaccc ggagctcccc tcgcaggcct ttgatgttat ggccagtcta
    10081 gtcaacgatt gcgccgcccg cggaacggcc gagcaggttg cgctgtgacg ggcaggcatc
    10141 gaggacgccc ggtagaagat ctgtctcggg gcgtgttcga cttgctggtg attggtggcg
    10201 gcatcgtcgg ggccagggtt gctttcgacg cggcgcgggc agggcttcgt gtggcgctgg
    10261 ttgacgccgg ggactttggt ggggcgacat ccggggcttc agcgcgcctg gttcacggtg
    10321 ggcttcgcta ccttggaacc ggcgattttc ggctcgtgcg catggccctg agggagcgta
    10381 gcgtcctggc ctcccgcgtc gcgccgcatc tcatcacgcc gctgcccttc gtgttgtgtg
    10441 cggcagaagg acggcgctcg aggtgcgcgg cgggattgct cgtctacgca gcactcgacg
    10501 ggttcaggag cccgcttccc cgtttcgtca ccccggagga ggcttccttc ctcgtgcctt
    10561 ccctgtgtgt aggagagtcc gcttcacacg ccgtctacta cgaggcggcg accaacgata
    10621 gccggctggc actagccacc gtgacggcgg cggcgcgcgg cggcgctgtg gtagcgaact
    10681 atctgcgggc cgtggatctg gcgccgggca ggatctcacg cgtgtcgcta gagggacgca
    10741 ggggagaggt cacagtcagg tgcagggccg tggtcaatgc cacgggtccc tggttggacc
    10801 tcctgaggaa catggaggat ccagcatgcg agccgatcac ccgcctgagc aaaggtatcc
    10861 acgtcgtact aaggccggac caacagtgga ggggggccgc cgccgtctcc ctacacgacg
    10921 gacagcacct ctacgccgtt ccttgtgagg acaggattct gctcggcacc accgagcagg
    10981 agtaccaggg tgaccccacc gctgtgactg cggagcccgg ggacgtcagc tacctcttgc
    11041 aggtggccaa ccagttcctg cgctctgaga tgctgcgccg cgaacgtgtg gcctcggctt
    11101 tcgcaggact gcgcgtcttg ccgcgggggg aagaagcgac gctccatgcc agcaggggtc
    11161 acctcctgag cgtcgggccc ggcggtatgg tctcggtggc cggaggcaag ctgaccacgc
    11221 accgccgaat cgccctcgat gccctgcgac acctgcccga cagggtcaga cccaggagac
    11281 tctacctcag cgacgcacct ctccccggtg cttcccctgc tccggtgcac cactctcacc
    11341 tcgacggttc gacgctggat cacctcgtgc acctctacgg ctcagagacc ggcacgctgc
    11401 tcggacagtg caaggtgcac tacaacgcgc tcgaaaggat aacggcggga gcgccggatc
    11461 tctgggcgca ggtctaccat gcggttcgcg aagagtgggc cttgagcgcc gaggacatca
    11521 tttaccgcag aacaagcctt gggctgcgtg gcttcgacac accagagctc cggcagagga
    11581 tctccgcagt tctcgaagcc gaagaccccg gacttttcga gccgaacttg agggagtcca
    11641 cccacctttc gagttcgtaa cggttgccgt aacgcttgga agacgatcgt actgtatggt
    11701 tcgggtgtga cggaaggagt tctaccctag tcattgcagc cttattcaat cctttgagac
    11761 gacgtatcca gtcgttcata gatcaccgcc tttaccttag caagtacgac gcgaggacgc
    11821 gccttagccg ccctcgttcg tcgcaggtcg tgggaggtcg gtacttgcgg tggtcctgaa
    11881 cttgtgtcaa cctcccgtaa tggcttacta ctcgtgttta tcgtcgatgg cccatgcccc
    11941 cacatccgcc tacggtcggg aaaattgacc accggacaaa gccccacaac ttgagcagga
    12001 agttcacgcg acagaagttc ctgggtgtcg cagggaccgg cgccgcgtac atcgcgctgc
    12061 tgagcttgcc agggtgcaga aaggaacagc agagtcacag ttctcccccg gcccagccgg
    12121 acaacgtgag gacgttccgc tcgcgcccgg acctcagtcc tccagccatc gagatacacg
    12181 agcgggcacg cggttccgcc cccggctaca tctttattgc cgcaaagaag ggtgccgggc
    12241 aggatggccc gatgatcgtc gacgatctag ggcggccagt gtggttcagc aaggacaggt
    12301 acgcgacgga cttcaaggtg cagcgctaca ggggggagcc ggtgctcacc tggtggcaag
    12361 gcgagatcgt ggccggacac ggcgaaggcg agtatgtgat cttcgacgac tcctaccgcg
    12421 agataactcg ggtccaggcg ggcaacggct accgcggaga tctgcacgag ttctctatca
    12481 cccagcagga caccgcgctc ttgacgctct acgtccagac gcaggcagac ctctccccga
    12541 tcggcggtcc caaggatggc atggtctggg aagggatcgc ccaggagctc gacctcgaga
    12601 ccgaagaggt gctgttcgag tggcgcagcc tggagcacgt gggcgtcgag gagtcctacc
    12661 gcgagccgcc gcaggagccc acaacgcccc tggactactt ccacatcaac tccatagata
    12721 tagacttcga cggcaactgg ctcatctccg ccaagggtac atccgccgtc tacaaggtgg
    12781 accgccagag cggcgagatc ctgtggcgcc tgggcggcaa ggagagcgac ttccagatgg
    12841 gagaaggcac caggaccgtc tcccagcacg acgcccgccg ccagcaagac ggcacgatca
    12901 ccatcttcga caacggcgcc cctcctcagg tgcacgagca gtctcgcacg atcgcggtgt
    12961 cactcgacat ggacgaaatg agcgccgccc tggtgcgcga gtatacccac ccgcagaagc
    13021 tgatcgccac ttcccagggc aacgcgcagg tcttaccgaa cggcaacgtg ttcgtaggtt
    13081 ggggtagttc gccgttcttc tccgagtaca ccaaagacgg caagctacac ttcgacgccg
    13141 agttccctgg cagcgcccag tcctaccggg cattccgctt cccgtggaaa ggagatcctg
    13201 tcgaggagcc ggccgtggcg gtcgatacgg gacctgacga cagggtgacc ctctttgcca
    13261 gctggaacgg cgccaccgag gtcgagagct gggaggccct cgccggcccc ggccccgacc
    13321 aactcgagcc cgtcggaacc tctccccggc atggcttcga gaccaccctc acgatgcgca
    13381 ccgccgggcc ttacgtcgcc gtacgggcca agagcgctgc agggcaggtg ctcggcacca
    13441 ccagggccat caagctggga gcccaaacgt cgtagagcgt gttcttgcct gtaccttcgt
    13501 tagtacggca agccgttaca tagctccgaa gtcgggtacc gcgaactggt ggtaacggct
    13561 cgttatcctc gggacgagga gcttaggaaa ggttagttct tcttaatcgg ttgttgcccg
    13621 atctgttacg tgcgtccgct acagtgccct tcaggctaag gacgggaggt agcttctgtg
    13681 ggagagagga cgaggctcgg gctcggggtt ctcggcgtcg ctttggtgct tggaggcctg
    13741 ggagacctac ttctccgcgc cacgccgtgg gggatcaacc tcctcatctg ggttacggct
    13801 atcgtcggcc tcgctatcct gctcgccaaa tgggggcgcg ttggtaccgg gggagagggg
    13861 aagtggctgg tattcgtcgc cctagtcttc gccgccggtc tggtcctgcg cgattctccc
    13921 gtggtggtgt tcctggatct cctcggaatc ctgatctcgc tctcccttgc ggtgtggctc
    13981 ggacgcaacg ggagcctgag gcgcgccggt gttctggact acattcttgg aggagtctat
    14041 accggtgtct tgacttcagc gggaccggta cccgtgagcg tcgccgacat agagtggcga
    14101 gaggcggtcc gcgggcgatg gaaggggcag gtgctggcgg tgtcgcgggg cgtactgctc
    14161 gctgcccccc tcttgctcct cttcggcgcg ctcctggtgg cggccgacgc catcttcgag
    14221 cggctcatca tcgaggtctt cggcttcgac ctcgccgagg tcttctcgca cctcgctctc
    14281 atcacgttct tcgcctggat ctcggcaggg gtgctctggg cggggctgat ggcacgcgtc
    14341 ccggagaact tctcgatccc acgtctcaga gtactctcgc tcggcatcat cgaggtaggg
    14401 atagtgctcg gtcttctaga cctgctcttt cttgccttcg tcgtgatcca ggtccgctac
    14461 ctgttcggcg gcgcggggcg cgtggccgcc acggcgggcc tgacctacgc cgagtatgcc
    14521 cggcgcggat tcttcgagct ggtcaccgta accgccctcg cgctccccct cttgctcgtc
    14581 gcccactggc tactcaggag cgggacccgc gcccacgagc gtctgttcaa ggcgctcgcc
    14641 gggataatgg tagccctgct cttcgtcgtc gtggcctccg cgctgcagcg gatgtacctc
    14701 taccagcaag agttcggcct gaccgagcta cgtctctaca cgacgatatt catggtctgg
    14761 atctccgtcg tcctactctg gttcgtcatc accgtactgc gggccaggcg cgaccgcttc
    14821 gcgtttggcg cccttctcgc cggcttcgcc gctatctttg ccataaacgc catgaacccc
    14881 gatgccctga tagcaagcac caacatagac cgcatggaag ctggaaagcg cttcgacgcc
    14941 tactatctga ccaccctgag cgccgacgcc gtgcccgtgc tcgtcgagtc tctgccccag
    15001 ataggcgacg accgcctgtg gaaagcctat accgtcgagc aggccgtcgt ggatcgctgg
    15061 tactccagag atgccgactg gcgtacgtgg aatctgggcc gttcgagggc ccgccacctg
    15121 gtcgagagtt acgtcgaaga ccaggaccta caggcatccc gggtgatcag acctgactcc
    15181 ggtcaccaag atagggcatt tgtgcaccgt cccgaccgag ttatccacaa catgttgtgg
    15241 ataagaagcc cgcaacgggg gataactgga tcaactgtaa caagctctta acaattaggc
    15301 gaaaacaaca agctgactag ctgacaagcg caggcgaagc ctgagcgtgc tgaaatgctc
    15361 tttcggcggc gcgtgtggac aagcgtgtcc gaggctttac aatcagcgta gagaacggat
    15421 gccgacaaga ggtgagagcg tgataaccga ggagcgcgtg caggaccagt tgcggaatgt
    15481 tatagaccct gagatcggga tggacctcgt cgagctcggc ctgatctacg acgttggcgt
    15541 gcatgacgag gggcgtcacg tcgatgttac cttcagcctc accagcccga tgtgccccgt
    15601 tggggatttg atccaggaac aggtcgagac ggagacgctc tccatagagg gtgtcgaaac
    15661 cgtgaacgcc cagctcactt tcgagccgat gtggagcccc gagatgatga gccccgccgc
    15721 caagctgttc ttcggccggt aggactgatc tgggcgtctg aaccttcgaa gataggttct
    15781 gtctgcgcct tactcaatct ctctagcgtc cacccagctc cgccacacgc tcccttatgg
    15841 cctcgacgac ctgctccctg ggtctggcat actcatgaaa accgctggga tcgatgggtt
    15901 cgccgaaggc gaccgtgacc tgctccaacc ttcgcaagaa ccttcctctc ggcatggcct
    15961 cgtaagtgct cggatgaaca cgggcacgac aggcaccgga tagtaactca acaacattcc
    16021 cacgcccggc ttgaacggct gcatgctgcc tgtcggtgaa cgttcacctt cggcgaacca
    16081 gaccaggttc tgtccgcgcc tgagcaccgc ggccccgaag gccaggctcg agaggcccgt
    16141 gcggccgagg tcgatcggga ctacctgcgc gagacggctt atgagacggg tcagcggatt
    16201 gccgaaggcg gcgccggtcc atccagccca ttaggtacgc cgcagtacgc tatagtcgag
    16261 cgcagccgcc accgcgaacg catccaggta gctgacgtgg ttcggtgcga tgatgaacgg
    16321 gccctcctca ggcaggttct ccgggccttc cacccgcagg cggaacgccc cacccatcag
    16381 tctccggttg aggaagaaaa tgctcctggc cagcgccgag cagaccggct ccagcggcct
    16441 cagccaccgc cgttgccggt cgtcgaggac ctcttcgggc atttcgagcg gtgaaaagcg
    16501 gcgtgtggtt cggccggatc ccgccgcgac ctcccgcagc aggtcgcgaa cggtgtcgat
    16561 attttctatc gcgtcttcgt ccagctccac gccggtcttc tcgccgatct ccagcgtcag
    16621 gttcacccat cccagcgaat cgatgtccag atcgagctgc atacccgtct cgggcgcaag
    16681 ccgcctgtct ggatagcgct cggtgaccat cttccagacg cccatcgccg cggtattctc
    16741 gagcaaccga tcaataatca gtatgtcctc cttacgttgt aaatttacaa cgtatatata
    16801 gagtggctca tcttcgattg tcaagggtct ttgagtggtc tatggaaagc gtgggaggat
    16861 gtgtatggaa tctggcaggt tgcctgtagg tgcagctgcg tagcgcgtgc cgacgatacg
    16921 caaataatct agaaacctga tgcctttagc cgcgctttac ggaaggaggg ttccgcagag
    16981 tgctttcaga ggtcgctgag cttgctggtc tcgaacctct ctagtactag ctcgaaggct
    17041 cctgtgccta tacgagcacc gcctgagaag ggatgctcca tggcgcctat ggtgaacagc
    17101 acaagcccga tcaccgccgc cagggtcccc accatcagcc tgtgcgccca cgtgctctcc
    17161 aggccgaaga ggtaggtgaa cccgatcgcc gcaatccccc cgaataccag gacggaccac
    17221 atgactccgg gaacaccttc ctctgccgcg accagacgcg tcctccttgc atcagccagg
    17281 gtatcgacct ggtcgagccc ttccgcgtaa agctgttcct cggcctttgt gcgcggctcg
    17341 aagttctgta agttctgccg gatgtcgtcg atgatggtcc acccactgga agccccccgt
    17401 gtctgcgtca tcaagggtgc ttgcccctgc tccatca
//
LOCUS       DATMVF010000209         7173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_530349, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000209 DATMVF010000000
VERSION     DATMVF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530349"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VN178_10460"
     CDS             complement(<1..942)
                     /locus_tag="VN178_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81434.1"
                     /translation="MMSTGWISDRRSRGAGVGVAACFLLTGIVFATWASRVPAIKGGL
                     DLSDGRFAIALLGLEAGAVFGLQLGGLLVPRVGSRRALSSTLVVFSGALLGPAFAPGL
                     PLLAASLFAFAALNSVVDVAMNTQGLALQRLMGRPVLSRLHAMHSLGGVLGAGAGALA
                     ARFGATPPQHFLACAVAAVVAGMAAWPLLLPSRFDAGEEGSEDGTEGAGGLRRWFGGW
                     SGYIALLGALAFCFTLAEGAGLNWSAVYVTESLGGTEAFGAVALGVFLGAVTLGRLVG
                     DRLVSRFGAVRVFRAGAIVAGGGFGGALLVDTPVAGMV"
     gene            1191..1763
                     /locus_tag="VN178_10465"
     CDS             1191..1763
                     /locus_tag="VN178_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS81435.1"
                     /translation="MFGPLVGVVEDEELIRRVAEGDDRALSELYDRYSRPVYATGIRV
                     LGDASLAEDLVQDAFMSVWRAAGSFDPGRASFATWLYRIARNRAVDLNRRRRVRPLSA
                     GEEPLRNVSGGPEPEGGVDGWDVARALSRIPDEHREVLTLAYFEGLSQREISRRTGIP
                     LGTIKSRTTSALRRLHRSLVDPAAEESRHD"
     gene            1756..2424
                     /locus_tag="VN178_10470"
     CDS             1756..2424
                     /locus_tag="VN178_10470"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWS81436.1"
                     /translation="MTEDRFEELLGPYLLGELSVEEERELQRHLEECSGCRDELARTR
                     QTHDLLRGLATGGPPPELKDRVLAQAGGEIPASSRGAWRLWVPAAALLVVAVLGAGLL
                     WTITQDSSEGIPLTATVPASDGGGEISGEMEEGNLKIELVVWGLPELHKGEYYELWYA
                     KEDGGRISCGTFRAQQGDQTTVNLTAPANAVSYPEIEVTREPDDGDPGSSGEKVLVGD
                     LRSL"
     gene            complement(2643..4175)
                     /locus_tag="VN178_10475"
     CDS             complement(2643..4175)
                     /locus_tag="VN178_10475"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HWS81437.1"
                     /translation="MGSRFTRRWFLKAAGAGAAYLALAGTVGSEPVESYPKVRSVRTP
                     RVKPLLGVPFPPQDGNWAFRSRPDLNPPAVEVSTQAHEDTAPGYIFLAPEKGGAGKGG
                     TMIIDDHGQVVWFRPLRGPFGRTMNFGTQTYQGREVLTWGQTPGEYIIFDSSYREIAS
                     FRAANGYDGDHHEFLISPQDTALITIYEMVPRDLTSVGGSKDGVAMQGIVQELDIETG
                     EVLFEWKSIDHVDLEETYGTPSEDHYPGIDYFHINSIDIEPDNNLLISARETSTVYKV
                     DRTSGRIMWRLGGKKSDFEMGPGTRFAFQHDARRLPDGTISIFDNGSLVFEDGIPKAV
                     EESRAIVLDLDVDRMRASLVREYTHPDKQYAHAAGNTQVLPNGNVFVGWGRGLAVSEF
                     DDDGKLLFDFKVSPEHKSYRSFRLPWSGQPFRPPIAVAERTSANELVVYASWNGATDV
                     ALWEVLAGPDPAQLESLGSILRDGFETVMLVQTSDPYVAVRAKDRSGRVLGASTPVKA
                     KG"
     gene            4720..5592
                     /locus_tag="VN178_10480"
     CDS             4720..5592
                     /locus_tag="VN178_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS81438.1"
                     /translation="METTATRRRPVRNDETPDLIPDYFARIDKGKLLTYEEEVELSRR
                     ARIGDRKARQRLIEKNLRLVVSVAKKYRGMGLPFEDLIQEGNIGLMKAIDKYDPERGF
                     RFSTYATWWIRQAVQRAVADKGRTIRVPVHMGEKIRKMARAYNVLSEELRREPSDEEV
                     AERLEWTPERVREVKDALPDATSLNQALSSEEGASEIGDFVEDERASDTAGEVLREME
                     TSGLAAAIDGLPERQRYVLVRRYGLGVDEKPATLAQLGDELGVSKERVRQLQREAEGM
                     LREGHYESALSVAA"
     gene            complement(5764..5988)
                     /locus_tag="VN178_10485"
     CDS             complement(5764..5988)
                     /locus_tag="VN178_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HWS81439.1"
                     /translation="MAEKQDRDIERVYSTSEFVAKLRRLADALEAGERFEIQVAGERI
                     YVPARAEFNVEHEREGDEEELEFQLKWTHS"
     gene            6205..6399
                     /locus_tag="VN178_10490"
     CDS             6205..6399
                     /locus_tag="VN178_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81440.1"
                     /translation="MLETRENENESFEGGKYASTEEELYEKAIALLEKCKIEARHSGQ
                     KVPESTAEASPMTVQRPRGA"
     gene            complement(6498..>7173)
                     /locus_tag="VN178_10495"
     CDS             complement(6498..>7173)
                     /locus_tag="VN178_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HWS81441.1"
                     /translation="TAFFVARRRDRLAGVPVRALLPYAFLLALLAATNGIAALRSVLE
                     ELGPVFDGPGLPLLASGAFAALLLGLGGGEIGPATRRTATQWLPVAGAVLTFVLAGEV
                     VAESGAAGLLAGGAEALGVFFPAVSPVLAALGGALTGSNAASNALFMPLQVEAARGLG
                     FSEALTAAVQNVAGSHASMLAPQRMVLAATAAGLLGREGDILRTSVAPVGVSISLLAL
                     LGMLIR"
BASE COUNT         1402 a         2220 c         2325 g         1226 t
ORIGIN      
        1 aaccatcccg gcaaccgggg tgtccaccag cagcgcgccg ccgaacccgc caccggcaac
       61 gatcgcccct gcccggaaca cccgcacggc gccgaagcgg gagacgagcc tatcaccaac
      121 caggcgcccg agcgttacgg cgcccaaaaa gacaccgagg gctactgcgc cgaaggcctc
      181 cgtccctcct aacgactccg ttacgtacac ggcggaccag ttcaggccgg cgccctccgc
      241 cagcgtgaag cagaacgcca gtgcccccaa caaggctatg taacccgacc agccaccgaa
      301 ccaacgtctc agtccgccag cgccttccgt tccatcctcc gagccttcct cccccgcgtc
      361 gaaccgcgac ggcaagagca ggggccaggc ggccatacca gccaccaccg ccgctacggc
      421 acacgcgaga aagtgttgcg gcggtgtggc gccgaagcgc gccgcaaggg cgcccgctcc
      481 cgcgccgagg acgcccccca ggctgtgcat ggcgtgcagc ctggagagca ccggacgacc
      541 catgagccgc tggagcgcga gcccctgggt gttcatcgcg acgtccacga cgctgttgag
      601 ggcggcgaac gcgaacaggc tcgcggccag caagggtagg cccggagcga aggcaggacc
      661 gagtagcgcg ccgctgaaca cgaccagcgt actgctcaac gcccgccgac tccccacgcg
      721 tggcaccagg aggccgccta gctggaggcc gaaaaccgcg cctgcctcca acccgagaag
      781 ggcgatggcg aaccggccgt cgctcaagtc cagaccgccc ttgatagccg ggacgcgcga
      841 agcccaggtg gcaaatacga tccccgtgag tagaaagcag gccgccaccc cgaccccagc
      901 cccgcgcgaa cgtcgatcgg agatccatcc cgtactcatc acgtcacact ctaaattgtt
      961 ttgatcatga caacaaggcg tggcacgcgg tcctgaacga accattgggc ggcatctttg
     1021 cgtacatacc gtagagcgaa cggccaggat gctttggagg gtactatcac gagttttggg
     1081 tgtgggcttg ggagcgatga cccggtcgaa accgtcaggt agttctgtgg tcgatgaggc
     1141 ggaggccgga gtgtggcttg accggctagc ataggactcc cgggagggag atgtttggac
     1201 cgctagtggg ggttgtggag gacgaagagt tgatacgccg cgtagcggaa ggggacgacc
     1261 gggcgctctc ggagctctat gaccgttact cgcggccggt atacgccacc ggcatcaggg
     1321 tgcttgggga tgcttctctg gccgaggatc tggtgcagga tgcgttcatg agtgtctggc
     1381 gggcggcggg atccttcgac cccggtcggg cgagcttcgc gacctggctc tacaggatcg
     1441 cccgcaaccg ggcggtggat ctcaatcgca ggcggcgggt aaggcctttg tctgccggtg
     1501 aggaaccact tcgaaacgtg tctggcggcc ccgagcccga gggaggggtg gacggctggg
     1561 acgtcgccag ggcgctctcg cgcatccccg atgagcacag ggaagtgttg actctggcgt
     1621 acttcgaagg actctcgcag cgcgagatct cccgccggac cggcatccct ttgggaacga
     1681 taaagagccg gaccacgtcc gccctcagga gactccaccg gagcctggtc gatcccgcgg
     1741 cggaggagtc tcgacatgac tgaagaccgc ttcgaggagc tcctcggccc ctatttgctc
     1801 ggggagctat ctgtcgagga ggagcgtgag ctgcagcgtc atctagagga gtgttccgga
     1861 tgtcgggatg agctggctcg cacccgacag acccacgacc tcctccgagg attggccacc
     1921 ggcgggccgc cgcctgagct caaggaccgt gtgctggcgc aggcaggggg cgaaataccg
     1981 gccagctcca gaggggcatg gagactctgg gttccggcgg cggcgctcct ggtcgtcgcg
     2041 gttcttggcg ccggtctctt gtggacgatc acgcaagact cctcggaagg cattcccctg
     2101 accgcgacgg tacccgcctc ggacggtggt ggcgagatca gcggagagat ggaagaggga
     2161 aacctcaaga tcgagctggt ggtgtggggc ctgcccgaac tccataaggg cgaatactac
     2221 gagctttggt acgccaaaga agatggcggt cggatcagct gcggcacctt ccgggcgcag
     2281 cagggagacc agacgaccgt gaacctcact gcccccgcca acgctgtgtc gtacccggag
     2341 atcgaggtga cccgcgagcc cgatgacggt gaccccggct caagtggtga gaaggtcctg
     2401 gtcggagatc ttcgaagtct gtgagctgtt agcacttcag ctagtcggcc cgcctgtttg
     2461 gcaggctttc agccctcggc gagatcgcct gagagcgtcg ccaacagcgt agaacccgat
     2521 cgcgcaggtg gtggttactc ttacgctgca cagtgaggcc agaacgggag aagctgaagg
     2581 ctgagagcga ggctaagccg aagcgagctg acaagcggaa gccaaaggct ggagcgtgct
     2641 gactagcctt tcgctttgac gggcgtggag gctccgagga cgcgtcccga acggtctttg
     2701 gctcgcacgg cgacgtacgg atcggaggtc tgcaccaaca tgaccgtttc gaatccgtct
     2761 cggaggatgg atcccaggga ttctagttgg gcgggatccg ggccggcgag tacctcccag
     2821 agggcgacat ccgtagcccc gttccagctg gcgtagacta ccagttcgtt tgcggaggtg
     2881 cgttccgcaa ccgcaatggg cggacgaaac ggctggccgc tccacggaag gcggaaggac
     2941 cggtaggact tgtgttcggg cgataccttg aaatcgaaga gcaacttccc atcatcgtcg
     3001 aactccgaga cggccagtcc cctgccccac cctacgaaca cgttgccgtt cggaagcacc
     3061 tgggtgtttc cggccgcgtg ggcgtattgc ttgtcggggt gggtgtactc gcgcaccagc
     3121 gaggcgcgca tcctatctac gtcaaggtcc agcactatgg cgcgggactc ttctaccgcc
     3181 ttggggatgc cgtcctcgaa gaccaggctg ccgttgtcga agatgctgat tgtgccgtcg
     3241 ggcaggcgtc gggcgtcgtg ctggaaggcg aatcgggttc ccggacccat ctcgaagtcg
     3301 ctcttcttcc cgccgagccg ccacatgatt cgaccgcttg tgcggtcgac cttgtagacg
     3361 gtggaggttt ctcgggcaga tatcagcagg ttgttgtcgg gctctatgtc tatggaattg
     3421 atgtgaaagt agtcgatgcc agggtagtga tcctccgacg gtgtgccgta ggtctcctcc
     3481 aggtccacgt ggtcgatgct tttccattcg aagagcacct cccccgtctc gatatccagt
     3541 tcctggacga tgccctgcat ggctacgccg tctttggagc cgccgacaga ggtgaggtcc
     3601 cgtggcacca tttcatagat cgtgatcagc gcggtgtcct gcgggctgat gaggaactcg
     3661 tggtggtccc cgtcgtatcc gttggcggcc ctgaagctgg ctatctcgcg gtaggaactg
     3721 tcgaagatta tgtactcgcc cggtgtttgt ccccaggtca gtacctctct gccctggtag
     3781 gtctgcgtcc cgaagttcat cgtgcgcccg aaaggaccgc gcaacggacg gaaccacact
     3841 acctgcccat gatcgtcgat gatcatcgtg ccgcccttac cggcgccgcc tttctcaggg
     3901 gcgaggaaga tgtagccggg ggcggtgtcc tcgtgcgcct gcgtggacac ctcgacggcc
     3961 ggcgggttga ggtcagggcg tgagcggaag gcccaattcc cgtcctgtgg cgggaatgga
     4021 acgcctagca ggggtttgac ccttggggtg cgtactgacc tgactttcgg gtagctctcg
     4081 acgggttcgc tgcccacagt gcctgccaac gccaggtacg cggcccctgc gcccgccgct
     4141 ttgagaaacc agcgtcgcgt gaacctagag cccatggcgg gcacccttca ccgcgagtac
     4201 tccgctgcga attcgtcccc gtcaaacgag ggacgctcct gcccgcctat gatcgcctcg
     4261 tagcccggga cgacgtcgtc tggcgcggtc ggatacgtaa tttctcggcc gtcatatggt
     4321 cctcccagga tagctatcac tgcgatgcca agtgtgcact atacgagatc ccgccgcaga
     4381 agcatatcga catacggcct gagaatctaa cgggtttcct ggtttcgatc cgtgcatagc
     4441 cttcagggta tcgtcggttc cggagcgaga gactactggc cgaacctggt cgcctcggcc
     4501 cgagagccct accagacacc ttgacgaatc ctccccttcg agtaaaatat acgtacattc
     4561 agatgtatag agcatcacga ttcccgtcac agattcaaaa tccgcgcaag agtaaatcgt
     4621 cagagcgatg ttcgtcgatt ctgctgatga acgggctgtt gaagaatcgg gctctagcgt
     4681 cgtttctagg gactcacagg gcttgtaagg aggtaagggg tggaaaccac cgcaacgagg
     4741 cgacgtccgg ttcgcaacga tgaaacgccg gatctcatcc cagattactt cgccaggatc
     4801 gacaaaggca agcttctcac ctacgaggag gaggtagagc tcagtcggcg ggcgaggatc
     4861 ggcgaccgta aggcccgcca gaggctcatc gagaagaatc tgagactcgt ggtctcggtc
     4921 gcaaagaagt accgtgggat gggcttgccc ttcgaggatc tcatccagga gggcaacata
     4981 ggcctgatga aggccataga caagtacgat cccgaacgcg gattcaggtt ctcgacctat
     5041 gctacgtggt ggatcaggca ggccgtccag agggcggtgg ccgacaaggg gcggaccata
     5101 agggtgcccg tgcacatggg cgagaagatt aggaagatgg cccgcgccta caacgtgctc
     5161 tccgaggagc tcaggcgcga gccctccgac gaagaagtcg ccgagcgtct ggaatggact
     5221 ccggagcgcg tgcgcgaggt caaggacgcg cttcccgatg caacaagcct aaaccaggcc
     5281 ctgagcagcg aggaaggcgc ttcggagatc ggcgatttcg tcgaggacga gcgggcttct
     5341 gacacggcgg gtgaggtgtt gcgcgagatg gagacctccg ggctcgcggc ggccatagac
     5401 ggcctacccg aacgccagcg gtacgtgctg gttcggcgct acggcctcgg tgtcgacgag
     5461 aagcccgcga ccctcgccca gctcggcgac gaactggggg tctccaagga gcgggttcgc
     5521 cagctgcaga gagaggccga agggatgttg agggaaggtc attacgaatc ggccctcagc
     5581 gtggcggcct aaacagatcc caaaggctct gatcgaggga gggaacccca aggggggcga
     5641 tccgtcctaa gcctcaactt ccgagacaga tgtgaaggac gcgcaagctc acatttctag
     5701 atccctcaga ccatctctac gtcgagcgcc tcaagggtat ggcgtgtggc atggagtaag
     5761 tgctcaggag tgggtccact tgagttggaa ctcgagctct tcttcatccc cctcccgctc
     5821 gtgctcgacg ttgaactcgg cgcgggcggg tacgtagatc ctctccccgg caacctggat
     5881 ctcgaaccgc tcgcccgcct ccagcgcatc ggcaagcctg cgcagcttcg ccacgaactc
     5941 cgaggtggag tagaccctct cgatgtccct gtcctgcttt tcggccatcc atcctcccct
     6001 gcttcgctaa ctctcgactt ccctgtagct taaagggttc gagggtccca gccaagtcag
     6061 accaggttta tgcgaatccg accttgacca gggggcgtgc gggtaacttg gtcgggtcga
     6121 accctcggtc gggttatcag gctcttaccg gaatgttgca attctgtaac tttggggcgc
     6181 gctgcaacgt gtagcctcat attcatgctg gaaacccgcg aaaacgagaa cgaaagcttc
     6241 gagggcggta agtacgccag tactgaagaa gagctctacg agaaggccat cgctcttcta
     6301 gagaagtgca agatcgaagc ccgtcattcg ggccagaagg taccggagtc taccgccgaa
     6361 gcatcgccga tgaccgtcca acgcccacgc ggagcctgaa ccgggagtag tctttaagag
     6421 gccgagtcgt cggtcgtcgc ggaccgccag gtcgaggcgg cgcggggtcg gcaagaacac
     6481 cagatccgac caacgctcta acgtatgagc atcccgagca gcgctagcag gctgatggag
     6541 acgcctacag gggcgaccga ggtccgcagg atgtcgcctt ccctgcctag tagtcccgcg
     6601 gccgtggccg cgaggaccat acgctgcggg gcgagcatgc ttgcatgact gccggcgacg
     6661 ttctgcaccg cagcggtgag cgcctcagag aagccgaggc cccgcgccgc ctcgacctgg
     6721 agcggcatga acaacgcgtt cgaggccgcg ttggagccgg tgagggcgcc gccgagggcc
     6781 gcaagcacgg gcgagaccgc gggaaagaag accccaaggg cttccgcgcc cccggcgagc
     6841 aggcccgccg caccgctctc tgccaccacc tcccccgcca gaacgaacgt cagaacggcc
     6901 ccggccacgg gaagccactg cgtcgcggtt ctccgcgtgg cggggccgat ctcgcctccg
     6961 ccgagaccca gcagcaacgc cgcgaacgcg cctgatgcga gcaacgggag gccagggccg
     7021 tcaaagacgg gacccagctc ttcgaggacc gaccgcaacg ccgcgatacc gttcgtcgcc
     7081 gcgagcagcg cgagcaggaa agcgtaaggc agtagcgcac gcaccggaac tcctgcgagc
     7141 cggtcacgcc tcctcgccac gaagaaggcg gta
//
LOCUS       DATMVF010000210         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_70563, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000210 DATMVF010000000
VERSION     DATMVF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70563"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VN178_10500"
     CDS             complement(<1..889)
                     /locus_tag="VN178_10500"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81442.1"
                     /translation="MWLLQRIGERLLGPVDRLQRAFHRSPPAFRRGAAMLVDALIVVE
                     SFAVVLIVRVSGDVEAPPWPFFWPFAVFSALAFVLLLNANGVYQSILRYTGIYQGVRV
                     LSATAIAAGGLFLTIFCVGPGGFEITRGNLVPLSVPLVGSVLAYVQLVAVRLYPRVFY
                     EMSLREVGRRTRTAIVGTGEPGVALAGHIWRTSAAETQVVGFVSTSPAEVGRHIEGSP
                     VLGVVEDLEDLIAQHGIDQVIIAMPQASREDMDWIWRTSIRASAEVKVMPDLGEYLAE
                     GAIKLRELQIEDLLGREPVD"
     gene            complement(927..1910)
                     /locus_tag="VN178_10505"
     CDS             complement(927..1910)
                     /locus_tag="VN178_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81443.1"
                     /translation="MTGFFAGSVAFLVAGAFVPLLVRFAVGRNLLDVPNLRSSHEVPT
                     PRLGGVAIFLGTLAGVALLRPEGLWPLLAAATLVWAVGLADDLSNLHFGVKAVLQALA
                     AAGLLLFYPPTIVSHAPGVFGVALVFVVGVFWIVALSNAFNFMDGIDGFTGGVAIVNA
                     LFLALLAGPAGGFLPALIGATAGFLIWNINPASIFLGDSGAYFLGFSLAAVALYVPAL
                     SGQEWTPLGFLACAAVFTPYLFDTAYTLFRRLRSGAGKNIFLAHREHIYQRITPTTGM
                     HRRTSNLYYGASVIAGFAALLISRGDVLSLAGVALVLACCALLAALPKLIG"
     gene            complement(2010..>2295)
                     /locus_tag="VN178_10510"
     CDS             complement(2010..>2295)
                     /locus_tag="VN178_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81444.1"
                     /translation="PLAYLDLVKTAAAALGRSPRIVRLPIEPVRLALAAAERLRLPLP
                     FDSGQVMRLREDKAYPYERARRDLDYAPRSFGAGVALEVARLRSLGMVRS"
BASE COUNT          463 a          759 c          743 g          330 t
ORIGIN      
        1 tgtccacggg ctcgcgtcca aggaggtctt ctatctgaag ctcgcgcagc ttgatggcgc
       61 cctcggccag atactccccg aggtcgggca tgaccttcac ctccgcggag gcgcggatgg
      121 aggtacgcca gatccagtcc atatcctccc ggctggcctg gggcatagct atgatgacct
      181 ggtctatgcc gtgctgggcg atgaggtctt cgaggtcctc taccaccccg aggaccggcg
      241 aaccctctat gtgtctgcca acttccgcgg gcgaagtact gacgaaaccc acgacctgcg
      301 tctcggccgc cgacgtgcgc cagatgtgcc cggcgagcgc cacgccgggc tcgccggttc
      361 cgacgatggc cgtccgtgtc cggcgtccga cctccctcag agacatctcg tagaagaccc
      421 tgggatagag ccgcacggcg acgagctgca cgtatgcaag caccgagccg acaagaggca
      481 ccgacagcgg gacgaggttc cccctcgtga tctcgaatcc gccgggaccc acacagaata
      541 tagtcaggaa gaggccaccg gccgctatcg cagtcgcgct cagaacccgc acaccctggt
      601 agatgccggt gtagcgcagg atgctctggt agaccccgtt tgcgttcagg agcaggacga
      661 acgcgagcgc cgagaatacg gcgaagggcc agaagaacgg ccagggcgga gcctccacat
      721 ccccggagac gcggacgatt agcaccaccg caaacgactc cacgactatg agggcgtcga
      781 cgagcatcgc cgccccgcgc ctgaacgcgg gcggcgagcg gtggaaggcc ctctgcaacc
      841 tgtcaacggg acccaggagc ctctccccga tcctctgtag aagccacata gtcctacatt
      901 ttaacttcta tggctggata cggccatcag cctatcagct tcggtagtgc cgcaagcagg
      961 gcgcagcaag ccagtacgag cgcgacgcct gctaaggaca ggacgtctcc ccgggagatc
     1021 aacagcgcgg cgaaaccggc gatgacgctc gccccgtagt agagattgct cgtacgcctg
     1081 tgcatgccag tggtcggtgt gatccgctgg tagatgtgct ccctgtgcgc caggaagatg
     1141 ttcttcccgg ccccgcttcg cagtcggcga aacagcgtat atgcggtgtc gaagaggtag
     1201 ggcgtgaaga cggccgcaca ggccaggaag cctagaggcg tccactcctg ccccgagagc
     1261 gcggggacgt agagagctac cgcggccagg ctgaagccga ggaagtaggc tcccgaatcc
     1321 ccgagaaaga tggatgcggg gtttatgttc cagatcaaga accccgcggt agccccgatg
     1381 agcgcgggca agaaaccacc cgccggtcct gcgagcagcg cgaggaagag cgcgttgacg
     1441 atcgcgacgc cccccgtaaa accgtctata ccgtccatga agttgaaggc gttgctgagg
     1501 gccacgatcc agaacacccc gacgacgaag accagggcga cgccgaacac gccgggagcg
     1561 tgcgagacga tcgtcggcgg atagaacagc agcaatcccg cggcggccag agcctgcagg
     1621 acggccttca cgccgaaatg gaggttggac aggtcgtcgg caagccctac ggcccagacc
     1681 agggtcgccg ccgcgagcag cggccacagt ccctccgggc gaaggagcgc cactcctgcg
     1741 agggtcccaa ggaagatggc gacacccccg agcctgggtg tggggacctc gtgcgagctg
     1801 cggaggttgg gcacgtcgag caggtttcgc ccgacggcga aacgtacgag cagcggtacg
     1861 aacgccccgg cgaccaggaa tgccacgcta ccggcgaaga acccggtcat cgtggctccc
     1921 gcctcccaag caaacaggct cgtgatcgcc gggctctggc cacgccgact cctctctcgc
     1981 cacacgattt cagcatagca ccctacgtac tacgaccgca ccatcccgag gctccgcagg
     2041 cgcgcgacct cgagtgcgac acccgccccg aaggagcggg gcgcgtagtc gaggtcgcgc
     2101 ctggccctct cgtaagggta ggccttgtct tcacgcagcc gcatcacctg gcccgagtcg
     2161 aagggcagag gtagccgcag cctctccgcg gcggcgagag ccaggcggac cggttcgata
     2221 ggcagcctga cgatgcgtgg gctcctaccg agggccgctg ccgcggtctt cacgagatcg
     2281 aggtacgcca ggggt
//
LOCUS       DATMVF010000211        20643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_356714, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000211 DATMVF010000000
VERSION     DATMVF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 20643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20643
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356714"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /gene="tuf"
                     /locus_tag="VN178_10515"
     CDS             <1..142
                     /gene="tuf"
                     /locus_tag="VN178_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWS81445.1"
                     /translation="EMVMPGDNTVMNVELISPIAMDEGLNFAIREGGRTVGAGVVTEI
                     VE"
     gene            146..457
                     /gene="rpsJ"
                     /locus_tag="VN178_10520"
     CDS             146..457
                     /gene="rpsJ"
                     /locus_tag="VN178_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWS81446.1"
                     /translation="MAVSKIRIKLKAYDHEVIDKTARSIVETAERTGAFVFGPVPLPT
                     KRSRYTVIRGPYRHKDSREHFKLHTHKRLIDIQQPTPRTVDSLQRLDLPAGVNIEIKA
                     T"
     gene            462..1079
                     /gene="rplC"
                     /locus_tag="VN178_10525"
     CDS             462..1079
                     /gene="rplC"
                     /locus_tag="VN178_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWS81447.1"
                     /translation="MATAVFGRKKQMTQAFQDDGTLVGVTVIEVEPNLITAVRTAEED
                     GYSAVQVGFGATKSGRVDKARAGTFAKLDTPPRRYLKELRDVSGEVGESIGVDVFEVG
                     DRVHVSATSKGKGFQGTIKRHGFARGPMSHGSHNIRQPGSVGASADPARIFKGQKMPG
                     QMGNQTATVRNIEVVAVDPDNNELWVRGGVPGGKNAVVRVRKVGD"
     gene            1081..1722
                     /gene="rplD"
                     /locus_tag="VN178_10530"
     CDS             1081..1722
                     /gene="rplD"
                     /locus_tag="VN178_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWS81448.1"
                     /translation="MAEGKLTAQVFDARSGDTSSLDLEGPRFTTEPNEHIIHRAVVAE
                     LDSRRRYTASTKGRSEIRGSGAKLYRQKGTGRARAGDAKSPIRQGGGTWGGPKAKPAR
                     YGKRINRKEATAAFDGALSAKAQDGELYVLDSLAFDTPSTKRAKELLSRMELEGPVLV
                     VLTESDANVALSFRNLPRVSVVGPHEYGVYELLRAREVVFSRAAYSRLAGKES"
     gene            1723..2010
                     /gene="rplW"
                     /locus_tag="VN178_10535"
     CDS             1723..2010
                     /gene="rplW"
                     /locus_tag="VN178_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWS81449.1"
                     /translation="MDPHQVIIRPVISEKSYNLIETERQYTFHVDRRANKNQIKRAVE
                     GAFDVSVQKVNTVNVKSKPKRQGLTRGRTPTWKKAVVKLAEGERIELFEGV"
     gene            2014..2853
                     /gene="rplB"
                     /locus_tag="VN178_10540"
     CDS             2014..2853
                     /gene="rplB"
                     /locus_tag="VN178_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWS81450.1"
                     /translation="MPARRYKPTSAGRRNSSVLTRDSVTKNDPEKRLTAKLHKTGGRN
                     NHGRITTRHIGGGHKRRYRLIDFKRRKDGVPATVAAIEYDPNRSANIALLHYHDGEKR
                     YILAPRNLTVGSIVENGPDAEVDVGNTLPLNRIPVGTVVHAVELEPGRGAQLARSAGT
                     SAQITAREGDLVTLRLPSGERRRVRAECRATVGVVGNQSHQNVRWGKAGRKRHLGVRP
                     TVRGTVMNPVDHPHGGGEGKSTPGRAPVTPWGKITQGSPTRKKHKRSDAMIVRRRRKG
                     RRR"
     gene            2854..3135
                     /gene="rpsS"
                     /locus_tag="VN178_10545"
     CDS             2854..3135
                     /gene="rpsS"
                     /locus_tag="VN178_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWS81451.1"
                     /translation="MTRSSKKEPFVEERLMERILRMNENNEKRMLKTWSRASVIYPEF
                     VGHTIAVHDGRKHVPVYCTESMVGHKLGEFAPTRTFRTHLKGDRTVRRR"
     gene            3132..3497
                     /gene="rplV"
                     /locus_tag="VN178_10550"
     CDS             3132..3497
                     /gene="rplV"
                     /locus_tag="VN178_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWS81452.1"
                     /translation="MKANAKYVRIAPRKARLVADEVRGKSFPEAVSLLRFTNKRAAKI
                     IGEILQSAAANAEHNDDADPEELFIKDIRVDDGPTIKRYRARAMGRATMIRKRTSHIS
                     IELGAPAGVLVGTPGDEEE"
     gene            3498..4166
                     /gene="rpsC"
                     /locus_tag="VN178_10555"
     CDS             3498..4166
                     /gene="rpsC"
                     /locus_tag="VN178_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWS81453.1"
                     /translation="MGQKVHPEGFRLGVQRKGEKVDFKSSWYSDRDFAELLGEDLKIR
                     DHIEKKLSRAGIADIQIRKAAEQGEIAVDIHTARPGIVIGKGGSEVDELRGELERLTG
                     KKVQVNVREVSRPELNASLVAESIAEQLVGRAAFRRTMKRALTSAIRSGAEGARIQLG
                     GRLGGIEMSRSETISEGRVPLHTLDADIDYGFREAKTQMGQIGVKVWINHGVHDEQPE
                     AIRR"
     gene            4170..4589
                     /gene="rplP"
                     /locus_tag="VN178_10560"
     CDS             4170..4589
                     /gene="rplP"
                     /locus_tag="VN178_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWS81454.1"
                     /translation="MLVPKKLKYRKPHRGRMRGKAKGGTDVQFGEYGLQALEPAWITN
                     RQIEAARIAMTRKIRRGGKVWINIFPHKPVTKKPAETRMGSGKGTPEAYVAVVKPGRV
                     MFEMSGVDETLAREAMHLAAQKLPIKTRFISRGGAQQ"
     gene            4586..4777
                     /gene="rpmC"
                     /locus_tag="VN178_10565"
     CDS             4586..4777
                     /gene="rpmC"
                     /locus_tag="VN178_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWS81455.1"
                     /translation="MKVAEVRELDVEELERRLAETRRELFNLRFQHATGQLENTGQLK
                     EVRKNIARILTVLNQKAGE"
     gene            4925..5191
                     /gene="rpsQ"
                     /locus_tag="VN178_10570"
     CDS             4925..5191
                     /gene="rpsQ"
                     /locus_tag="VN178_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWS81456.1"
                     /translation="MQTDTERNRRKERVGLIVADRADKTVTVSVEALVRHPMYKKRVR
                     RSRKFMAHDERNEARVGDTVRIVETRPLSARKRWRLANIISRAE"
     gene            5191..5559
                     /gene="rplN"
                     /locus_tag="VN178_10575"
     CDS             5191..5559
                     /gene="rplN"
                     /locus_tag="VN178_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWS81457.1"
                     /translation="MIQQESRLRVADNSGARSILTIRVLGGSKRRNAGVGDVIVATVK
                     EATPGSAVKKGDVVRAVVVRTKKETRRDDGSYIRFGENAGVIINPDGAPRGTRIFGPV
                     ARELREKGYTRIISLAPEVL"
     gene            5568..5879
                     /gene="rplX"
                     /locus_tag="VN178_10580"
     CDS             5568..5879
                     /gene="rplX"
                     /locus_tag="VN178_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWS81458.1"
                     /translation="MKLKRGDTVVVISGKEKGKRGEIERVIPKENRVVVGGVNVRTRH
                     ARPSQQNQQGLYHFEAPIDVSNVMLVDPNSGEPTRVGYRFTDSGEKLRVSKASGKDID
                     G"
     gene            5872..6414
                     /gene="rplE"
                     /locus_tag="VN178_10585"
     CDS             5872..6414
                     /gene="rplE"
                     /locus_tag="VN178_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWS81459.1"
                     /translation="MARLKERYNEEVVPNLQERFGIENPMRMPGLEKIVVNMGVGEAV
                     VNSRALDGAMEDLARITGQKAQLRRARKSIAGFKIREGMPVGARVTLRGERMWEFLDR
                     FISIALPRVRDFRGISPASFDGRGNYAVGLKEQVIFPEISYDSIDATRGLDVAIVTTT
                     ESDEEARELLRLLGMPFRQS"
     gene            6425..6610
                     /locus_tag="VN178_10590"
     CDS             6425..6610
                     /locus_tag="VN178_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWS81460.1"
                     /translation="MTRKALLVKQKKRQKYPVREYNRCQKCGRAHGYYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            6616..7014
                     /gene="rpsH"
                     /locus_tag="VN178_10595"
     CDS             6616..7014
                     /gene="rpsH"
                     /locus_tag="VN178_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWS81461.1"
                     /translation="MSVNDPIADFLTRIRNAHMAKQETVEIPHSKMKAEISRILLEEG
                     YVRDVTERGSGVEKRLVIALKYGPDGSRGIAGLRRMSRPGRRVYRKQTNIPRVLDGLG
                     VAILSTSQGILTDHQARRRGVGGEVLCFVY"
     gene            7025..7558
                     /gene="rplF"
                     /locus_tag="VN178_10600"
     CDS             7025..7558
                     /gene="rplF"
                     /locus_tag="VN178_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWS81462.1"
                     /translation="MSRIGKAPVEIPGAVSVELSGRSVKVNGPKGELTVPVASGIEVR
                     QEDGNVIVERAQDTPEHRAMHGLTRSLIYNAVTGVTDGFTKTLNIAGVGYRAALQGSD
                     INLQVGYSHPVSISPREGIEFEVPNATTIIVRGIDKQRVGQTAAEIRMVRPPEPYKGK
                     GIRYSDEQIRRKVGKAG"
     gene            7576..7926
                     /gene="rplR"
                     /locus_tag="VN178_10605"
     CDS             7576..7926
                     /gene="rplR"
                     /locus_tag="VN178_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWS81463.1"
                     /translation="MAVLEKRMHREKRRKRVRRKVAGTAVRPRLSVYRSNVHIYAQLI
                     DDDAGRTLAAADSREVGESQNRKDAARQVGALIANRASEAGIETVVFDRGGNKYHGRI
                     AALAEGAREGGLKL"
     gene            7946..8476
                     /gene="rpsE"
                     /locus_tag="VN178_10610"
     CDS             7946..8476
                     /gene="rpsE"
                     /locus_tag="VN178_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWS81464.1"
                     /translation="MGRPRTDNRQGGGRGRQRDGASDLQDRVVEIRRVAKVVKGGRRF
                     NFSALVVVGDGKGRVGTGLGKANTVPAAIQKGQERAKRNMFDVPMRNTTIPHEVTGKF
                     ESSSVLLKPASEGTGVIAGGGVRAVLELAGIKDVLTKALGSTTSVNLVQATVEGLKRL
                     RSKADIEQTRGVKITL"
     gene            8473..8655
                     /gene="rpmD"
                     /locus_tag="VN178_10615"
     CDS             8473..8655
                     /gene="rpmD"
                     /locus_tag="VN178_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWS81465.1"
                     /translation="MSPLKVTQVRSVVGSKRGHKRTVRALGLKRIRDSRVHQDTPQIR
                     GMVDKVRHLVHAEEVD"
     gene            8658..9119
                     /gene="rplO"
                     /locus_tag="VN178_10620"
     CDS             8658..9119
                     /gene="rplO"
                     /locus_tag="VN178_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWS81466.1"
                     /translation="MRLNEIKGAPGSTKARKRVGRGTGSGRGKTSGRGHKGAGARSGV
                     SAFAAYEGGQMPLQRRLPRLKGEARGRHTVARPTVYAPVNLGELKDLPGDTIGPDELR
                     AAGLVRKKAELIKILGQGDAGRAVTVKAHAFSRSAREKIEAGGGSVEVLER"
     gene            9167..10402
                     /gene="secY"
                     /locus_tag="VN178_10625"
     CDS             9167..10402
                     /gene="secY"
                     /locus_tag="VN178_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWS81467.1"
                     /translation="MRLLFTFAMLAAYRLGAYIPLPGVDPSVLAGGLQSSNPITGLFG
                     TFTGGAFNNFSVFSLGIMPYITAAIVMQLMTVAIPRLQELAKEGETGQQKITQYTRYF
                     TLALALIQSVAMVLFFRSAQGGGALAGTSPIEILVAIVTLTAGVMLTMWFGELISQRG
                     LGNGISLIITASILSQVPLAITALRENGDVLTVVILGLLAVMIVAAIVFVNEGQRRIP
                     ITYAKRQVGRRMSQGGTTYLPLKVNMAGVIPIIFASSLLLFPVVLAQLSAGGDPDSIL
                     GRLAAFFDPGNAPYLFLYALLIIMFTYFYTAVQFNPVEHADNLKKSGGYVPGYRPGQP
                     TAIYLNNVLTRITLFGAVFLAAIAVLPYLIAPALGLGNQLFLGGTSMLIVVGVSLDTV
                     RQLESQLMMRNYEGFLKKR"
     gene            10399..11028
                     /locus_tag="VN178_10630"
     CDS             10399..11028
                     /locus_tag="VN178_10630"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWS81468.1"
                     /translation="MRIILLGPQGAGKGTQAQRLAQEVGATHISTGDIVRAEIKSGSE
                     LGKKVQDYNDRGELVPDEIIVEMAKPYLDDADSWLLDGFPRNEAQAKALDQALEDIGE
                     DLDAVVALEAPDDALVVRLSGRRQSQDTGKIYHIEYDPPPESDPGPFIQRDDDAEEAI
                     RRRLQIYHDQTEPLKDYYADRDLLVTVDAQQEIPKVTQDILGAVGKEDA"
     gene            11025..11789
                     /gene="map"
                     /locus_tag="VN178_10635"
     CDS             11025..11789
                     /gene="map"
                     /locus_tag="VN178_10635"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWS81469.1"
                     /translation="MIVRKSTSELEAMREGGRITAGCLAMLEENVRAGVTTRELDRLA
                     EEFIHELGGKPEFKGYQGFPASICASPNAMIVHGIPGSYRLKSGDIISLDVGVRYEGF
                     VTDSATTVPVGYVPEDILGLLSTTRECLAAATDQMRAGKRLGDVGHAIQALAESRGYG
                     VVRDLVSHGVGRKMHEDPQIPNYGRPGTGPRLLPGMTFAIEPMITLGDYGIRLSEWDG
                     WSIYTADGSLSAHFENTVAVTENGPLVLTQNGSSGP"
     gene            11884..12105
                     /gene="infA"
                     /locus_tag="VN178_10640"
     CDS             11884..12105
                     /gene="infA"
                     /locus_tag="VN178_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWS81470.1"
                     /translation="MAKEDVIEVEGTVTEALPNTQFRVELENGHNVLAHISGKMRMNY
                     IRILPGDRVKVELSPYDLSRGRITYRFRT"
     gene            12116..12229
                     /gene="rpmJ"
                     /locus_tag="VN178_10645"
     CDS             12116..12229
                     /gene="rpmJ"
                     /locus_tag="VN178_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWS81471.1"
                     /translation="MKVRASVKPICERCKVIRRRGVVRVICTNPRHKQRQG"
     gene            12233..12604
                     /gene="rpsM"
                     /locus_tag="VN178_10650"
     CDS             12233..12604
                     /gene="rpsM"
                     /locus_tag="VN178_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWS81472.1"
                     /translation="MARIAGVDLPREKRVEVGLTYIYGVGRSTARKICRETQVDPDTK
                     VRDLAEGEVARVRSYIDQNCEVEGDLRREVNQNIRRLIDTGTYRGIRHRRGLPVRGQR
                     TKTNARQRKGPKPSIGGRKKR"
     gene            12605..13021
                     /gene="rpsK"
                     /locus_tag="VN178_10655"
     CDS             12605..13021
                     /gene="rpsK"
                     /locus_tag="VN178_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWS81473.1"
                     /translation="MGRQRQQRQRARGASRARRRVRRNVTTGVVHIKSSFNNTIVSVS
                     DQEGNVIAWESAGSLGFKGSRKSTPFAAQMTAESVANKAMDQGVRRVDIEVKGHGSGR
                     DMAARTFQSMGIEVLSIKDVTGQPHNGCRPPKRRRG"
     gene            13029..13631
                     /gene="rpsD"
                     /locus_tag="VN178_10660"
     CDS             13029..13631
                     /gene="rpsD"
                     /locus_tag="VN178_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWS81474.1"
                     /translation="MARYTGPRGRRDRRAGVMLSSMRKNPLEKKPYPPGEHGRGRRRQ
                     TEFGLRLMEKQKARWYYGVSEKQFRRAYDRATRMQGVSGENLLRLMELRMDNVVYRMG
                     FSTSRPQARQLVVHGHFQLNGRKHNVPSAILKPGDVITVKEKSRDLEPIQNAVENVVA
                     VPPWLEADHDNFTGRILHTPGRDEIDAPVEEQLIIEFYSR"
     gene            13666..14610
                     /locus_tag="VN178_10665"
     CDS             13666..14610
                     /locus_tag="VN178_10665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWS81475.1"
                     /translation="MLDIAPPRFRVEEEEGRRGTFVAEPLPRGLGHTLGNSLRRVMLS
                     GLTGAAVTKIRIEGVSHEFSTIEGVKEDVVDIILNIKGLKFKLEREEPIELTIAKDGP
                     GDVTAADIELKADVEVVDPEAYIASVSDGGHLDMRLTVERGQGYARAEQNKSEADPIG
                     VIAVDSLFSPVQRVNYMVSETRAGARADLDSLRMEVFTDGRIGPQEALQDASRQLMDF
                     LGLFTDGYQGAGAPDARQRGGRPVITDERPVEDLELTVRSYNCLKREGVDTIGQLATM
                     TEEELMNIRNLGMKSVDEIRSKLLEYGYSLESEGNEAR"
     gene            14597..14962
                     /gene="rplQ"
                     /locus_tag="VN178_10670"
     CDS             14597..14962
                     /gene="rplQ"
                     /locus_tag="VN178_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWS81476.1"
                     /translation="MRHAKRGRKLGREAQHRNLMLGTMAGQVITHGRIKTTAPKAKEL
                     RGVVDKLINTAKRDDLASRRQAVKVLKDKTVVRRLFEDVAPELDDRNSGYTRILKLGP
                     RLGDGAETVYLELVNHTVE"
     gene            14970..15830
                     /locus_tag="VN178_10675"
     CDS             14970..15830
                     /locus_tag="VN178_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS81477.1"
                     /translation="MQNPGEGTRPGGSFAVSCERVTFSYPGSAQPALSGVSFALRRGE
                     YVGVVGPNGGGKSTLVRLLNGLLKPESGRVLVSGRDPATEPFEVRKHLGVLFQNPENG
                     LVAPFVEDDVAFGLENLGVPREEMRERVARAVRAVGLQGYERREPHTLSGGEKQRVAL
                     AGLLAVEPEILVLDEPTSMLDAAGRREVLKHLGDLRFEKTVLHVTHHLEELLDADRIL
                     VLNGGELVADETPERLFSNADFLRENSLLLPVVPRLALELGFPPAKLRTPEELAEAIL
                     ARTQSGTRSL"
     gene            15827..16642
                     /locus_tag="VN178_10680"
     CDS             15827..16642
                     /locus_tag="VN178_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS81478.1"
                     /translation="MKLELRDVGHVYARGTPFQVEALRGVSLVVEPGEVLAIVGGTGS
                     GKSTLAQHMNLLLVPTSGEVLVDAVDATTLTKSELRRRVGLVFQFPESALFAPTVEED
                     VAFAPRQLGLDEEEVTERVLESLRALGAEELAARSPFALSGGEKRRVAIAGVLAMGPE
                     VLVLDEPTAGLDPAAREDLLALILGLREAGVSVVLVSHDLDEVAEVADRVCALHEGRV
                     SAVGTAAEVFYADPTRAPATVRVASLLRGASPGIGHPVRFRETLDALRAQQGT"
     gene            16644..17432
                     /locus_tag="VN178_10685"
     CDS             16644..17432
                     /locus_tag="VN178_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HWS81479.1"
                     /translation="MTTRSLGQFYPVSSPVHALDPRAKIIATAIFVVGLFLVDSTAGL
                     LFVAAALVALVAVSRIPAGTFLRLLRPVLYIVALTVLFQIFFSREGVILFEWGFLEVH
                     SGGLRMGLFLALRILLLVSSAGLLTATTAPVALADGIEDLLSPLKRVRFPAHEVAMMM
                     TIALRFIPTLGEEAEKIQRAQAARGADFSEGGPLRRARSFLPVLIPLTVGAFRRADEL
                     ADAMESRGYRGGEGRTRYRELRYRMSDAIALALTLLVLLVGIVL"
     gene            17429..18190
                     /gene="truA"
                     /locus_tag="VN178_10690"
     CDS             17429..18190
                     /gene="truA"
                     /locus_tag="VN178_10690"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWS81480.1"
                     /translation="MKLAGLVEYEGTGFAGWAAQPERRTVEGVLSGALSTVLRQPVKM
                     SVAGRTDAGVHASAQVVSFEADTALGPSLISYKTTAVLPKDVALRRCIAVPDEFDARA
                     DARSRTYEYRLVNDEIRSPLERHHAGYAPQRLDLELLSEAASLVEGTHDFRAFTPQKS
                     YHVRFERIVTGSRWTRQGDLLRYRITADSFLYGMVRTLVGTMLEVAGGKRDLPEFERL
                     LSGGKRSEAGPAVGSRGLTLVGVGYDDPNLWRSER"
     gene            18187..18855
                     /locus_tag="VN178_10695"
     CDS             18187..18855
                     /locus_tag="VN178_10695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HWS81481.1"
                     /translation="MNTGDTRELMQEIDTYVEDLFTPSDEILEAALRDSRRAGLPEIN
                     VSPNQGRLLQLLTGIAGARRILEIGTLGGYSTIHFARALPDDGALISLEIDEHHAGVA
                     RNNIERASLSEKVEIRVGDAHELLASLIEDEEGPFDVIFIDADKESYPEYLDASLRLV
                     RPGSLILGDNTIRGGSVLDPKNSAARATREFNERIARDPRLFSIALPLIREHIDGLTI
                     ARVL"
     gene            19069..19503
                     /gene="rplM"
                     /locus_tag="VN178_10700"
     CDS             19069..19503
                     /gene="rplM"
                     /locus_tag="VN178_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWS81482.1"
                     /translation="MMKSYMARPLEVERKWYVVDAEGKHLGRLATEIVRVLRGKNKPQ
                     YTPHVDVGDFVVVVNADRVAVTGRKAEQRVYRRHSGYPGGMKETSYEQMLARKPTEVL
                     RKAVYGMMPKTRLARKQFKKLKIYAGPEHPHSAQDPQTLEVT"
     gene            19504..19896
                     /gene="rpsI"
                     /locus_tag="VN178_10705"
     CDS             19504..19896
                     /gene="rpsI"
                     /locus_tag="VN178_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWS81483.1"
                     /translation="MAEALYNGTGRRKTAVARVRILPGDGKITVNGRDVDEYFPRPAY
                     RTAVRAPLELLGSAGRYEVTAKIEGGGPTGQAEALRHGIARALAEESQDARSELKAAG
                     MLTRDSRAVERKKYGLKKARKRPQFSKR"
     gene            19972..>20643
                     /locus_tag="VN178_10710"
     CDS             19972..>20643
                     /locus_tag="VN178_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81484.1"
                     /translation="MLGLRDLPRHGKGGRLNSGPISFGTDGVRGVANKGLLPEFALSL
                     GLAASRAFGGRILVGRDTRLSGEMLGSALCAGAASGGADVVDLGVLPTPGVAALAPRL
                     GATAAGVVSASHNPYPDNGIKFFSGEGRKLSPEKEREIEDLLGSDITRPTGSGVGRVE
                     VLGDATRMYAQWVLQRLRPPADGVKVLLDCANGAAYEVAPLVFGETGADLTLTGVDPT
                     GTNINE"
BASE COUNT         4218 a         6411 c         6953 g         3061 t
ORIGIN      
        1 agagatggtg atgcctggag acaacacggt aatgaacgtg gagttgatca gtcccatcgc
       61 gatggacgag gggttgaact ttgccatcag ggagggtggc aggacggttg gggcaggcgt
      121 cgtaacggag atcgtggagt aggttgtggc cgtatcgaag ataaggataa agctaaaagc
      181 ctacgaccac gaggtcatag acaagaccgc ccgctcgata gtcgagacgg cggagcggac
      241 gggggccttc gtgttcggcc ccgtcccgct gccgaccaag cgcagccgct acacggtgat
      301 ccggggcccc tacaggcaca aagactcgcg cgagcacttc aagctgcaca cgcacaagcg
      361 tctcatagac attcagcagc ccactccgcg cacggtcgac tcgctccaga ggctcgattt
      421 gcccgccggc gtgaacatag agataaaggc gacgtaggat catggcaacc gcagtattcg
      481 gacgcaaaaa gcagatgacc caggccttcc aggatgacgg aacgctcgtc ggcgtaaccg
      541 tgatcgaggt ggagccgaac ctgataacgg cggtccgcac cgccgaggag gacggctatt
      601 cggccgtaca ggtcggcttc ggggcgacca agtccggccg cgtcgacaag gccagggccg
      661 gcacgttcgc caaactcgat acgcccccgc gcaggtacct gaaggaactg cgggacgtaa
      721 gcggcgaggt gggcgagagc atcggggtag atgtcttcga ggtgggcgac agggtccacg
      781 tcagcgccac gtccaagggc aagggtttcc aggggaccat caagcgccac ggtttcgccc
      841 ggggcccgat gagccacggc tcgcacaaca taaggcagcc aggttccgtc ggggcatccg
      901 ccgacccggc caggatcttc aaaggacaga agatgccggg ccagatgggc aaccagacgg
      961 caacggtgcg caacatcgag gtcgtcgccg tagaccccga caacaacgag ctctgggtgc
     1021 gcggcggtgt gcccggcggc aagaacgccg tcgtaagggt ccggaaggtg ggtgattagg
     1081 gtggccgaag ggaagctcac ggcacaggtt ttcgacgccc gcagcggcga cacatcctcg
     1141 ctcgacctcg agggcccgcg cttcactacc gagccgaacg agcacatcat ccacagggcc
     1201 gtcgtcgcgg agctcgactc caggcggcgt tacacggcct cgaccaaggg gcgcagtgag
     1261 atccggggct cgggagccaa gctataccgc cagaagggga cgggacgcgc ccgcgcgggc
     1321 gatgccaaat cgcccattcg ccagggcgga ggcacctggg gcggcccgaa ggccaagccg
     1381 gcgcgttacg gaaagcggat caaccgcaaa gaggccacgg ccgcgttcga cggggcgctc
     1441 tcggcgaagg cgcaggacgg tgagctctac gtgctcgact ccctggcctt cgacacacct
     1501 tctacaaagc gggccaagga acttctgtcc aggatggaac tggagggacc cgtgctcgtc
     1561 gtcctgaccg aaagcgacgc taacgtggcg ctctccttca ggaacctccc cagggtcagc
     1621 gtggtaggcc cccacgaata cggggtctac gagctcttac gggcgcggga ggtagtcttc
     1681 tcgcgggcgg cctattccag gctcgccgga aaggagagct agatggaccc gcatcaggta
     1741 atcatcaggc cggtcatctc cgagaagagc tataacctta tcgagaccga gaggcagtac
     1801 accttccacg tggaccggcg ggcgaacaag aaccagataa agcgggccgt ggagggcgcg
     1861 ttcgacgtga gcgtccagaa ggtgaacacg gtcaacgtca agagcaagcc caagcgccag
     1921 ggtctcacca ggggcaggac gccgacctgg aagaaggccg tagtcaagct ggccgagggc
     1981 gagcgcatag agctgttcga gggggtctag actatgcctg cgagacgtta caagccgacc
     2041 agcgccggcc gcaggaactc ctcggttctg acgcgcgact cggtcacgaa gaacgatccc
     2101 gagaagaggc tgaccgccaa gttgcacaag acgggcgggc gcaacaacca cggccgcata
     2161 accacccgcc acatcggtgg cgggcacaaa cggcgctacc gtctcatcga cttcaaacgg
     2221 cgcaaggatg gggtgcccgc gacggtcgcg gcgatcgagt acgacccgaa ccgatcggcc
     2281 aacatcgccc tcttgcacta ccacgacggc gagaagcgct acatcctcgc gccgcgcaac
     2341 ctgacggtcg gttccatcgt ggagaacggg ccggacgccg aggtggatgt gggcaacacg
     2401 ctgccgctga accgcatacc ggtcggtacc gtcgtacacg ccgtcgagct cgagcctggc
     2461 cgcggggcgc agctggcccg cagcgcgggg accagcgccc agataacggc ccgcgagggt
     2521 gacctcgtga cgctcaggct cccgagcggc gagcgccgcc gggtcagggc cgagtgcagg
     2581 gccaccgtcg gcgtcgtcgg caaccagtcg caccagaacg tcaggtgggg caaggccggg
     2641 cgcaagcgtc acctcggggt caggcccacg gtgcgcggta cggtcatgaa ccccgtcgac
     2701 cacccgcacg gcggcggcga gggcaagagc acgccgggta gggctcccgt tacgccttgg
     2761 ggcaagatca cccagggttc tcctacccgt aagaagcaca agcgctcgga tgccatgatc
     2821 gtgcgccggc gcaggaaagg aaggaggcgc tagatgacgc ggtcctctaa gaaagagccg
     2881 ttcgtggagg agcgccttat ggagcgcatc ctccggatga acgagaacaa cgagaagcgg
     2941 atgctcaaga cgtggagccg ggcgagcgtg atctaccccg agttcgtcgg ccacaccatc
     3001 gccgtccacg acgggcgcaa gcacgtgccc gtgtactgta cggagtccat ggtcggccac
     3061 aagctcggcg aattcgcccc gacccgtacg ttccgcacgc acctcaaggg cgacaggacg
     3121 gtgaggcgcc gatgaaggcc aatgccaaat acgtccgtat cgccccccgc aaggcgcgcc
     3181 tcgtggcaga cgaggtcagg ggcaagagct ttcccgaggc ggtctcgctc cttcgcttca
     3241 ccaacaagcg ggcagcgaag atcatcggcg aaatactcca gagcgccgcc gccaacgcgg
     3301 agcacaacga cgacgccgac cccgaggagt tgttcataaa ggacataagg gtcgacgacg
     3361 ggcccaccat caagcgttac cgcgcgaggg cgatgggccg ggcgaccatg atccgcaagc
     3421 gcacgagcca catcagcatc gagctcggag cccctgccgg cgtactcgtg ggcacgcccg
     3481 gagacgagga ggaatagatg ggccagaaag tacatcccga gggcttccgc ctcggcgtcc
     3541 agcgcaaagg cgagaaggta gatttcaaga gcagttggta ttcggacagg gacttcgccg
     3601 aactcctcgg cgaggacctc aagatccggg accacatcga gaagaagcta agcagggccg
     3661 gcatagcgga catccagata aggaaggccg ccgagcaggg tgagatcgcc gtggacatcc
     3721 acacggcgag gccggggatc gtgatcggca agggcggcag cgaggtcgat gagttgaggg
     3781 gcgagctcga gaggctcacc gggaagaagg tgcaggtcaa cgtacgcgag gtctcccgtc
     3841 ccgagctcaa cgcgagcctc gtggccgagt ccatcgccga acagctcgtc gggcgcgccg
     3901 cgttccggcg gaccatgaag cgcgcgctca cgagcgccat ccgcagcggg gccgaaggtg
     3961 ccaggatcca gctgggcggg aggctcggcg gcatcgagat gagccggtcc gagaccatct
     4021 ccgaaggaag agtgcccctg cacacgctgg acgcggacat agattacggt ttcagggagg
     4081 ccaagaccca gatgggacag atcggggtca aggtctggat caaccacggc gtgcacgacg
     4141 agcagcccga agcgatcagg cggtagacga tgctagtacc caagaagctc aagtacagga
     4201 agccccacag gggcaggatg cgtggcaagg ccaagggcgg taccgacgtg cagttcggcg
     4261 agtacgggct gcaggctctg gagccggcct ggatcaccaa ccgccagata gaggcggcgc
     4321 gtatcgcgat gaccaggaag atccgtcgcg gagggaaggt ttggatcaac atcttcccgc
     4381 acaagcccgt gaccaagaag ccggccgaga ccaggatggg tagcggcaag ggcaccccgg
     4441 aggcttatgt agcggtcgtc aagcccggac gtgtgatgtt cgagatgagc ggtgtggacg
     4501 agacgctggc ccgcgaggcc atgcacctcg ccgcccagaa gctccccatc aagacccgtt
     4561 tcatctcgcg aggaggtgcc cagcagtgaa ggtagccgaa gtgcgcgagc tggatgtcga
     4621 agaactcgag cggcgccttg ctgagacgcg gcgcgagctc ttcaatctgc gcttccagca
     4681 cgcgaccgga cagttggaga acacgggaca gctcaaagaa gtcaggaaga acatcgccag
     4741 gattctgacg gttcttaacc agaaggcagg agagtaggta gtggtagagg aagagaaaaa
     4801 ccagactcct gagacgaccg atgagcctat ggccgaaacg ccgcaggacc tcgtagaggc
     4861 cggagaagcc ggcctcgatg ccggtcctgc caagggcccc gcgctggacc gggacgaggg
     4921 ccagatccag accgacaccg agcggaacag gcgcaaggag cgggtcgggc ttatcgtcgc
     4981 ggacagggcg gataagacgg tgacagtctc ggtcgaggcg ctggtgcgcc atccgatgta
     5041 caagaagcgg gtacgccgat ccaggaagtt catggcgcac gacgagcgca acgaggcccg
     5101 cgtgggcgat accgtaagga tcgtagagac caggccgctc tcggcgcgca agaggtggcg
     5161 gctcgctaac atcatatcga gggccgagta gtgatacagc aagagtcgag gctcagggtc
     5221 gcggacaact cgggtgccag gagcatcctg accatccgcg tgctcggcgg gagcaagcgt
     5281 cgcaacgccg gcgtcggaga tgtgatcgtg gccaccgtca aggaggccac gccgggcagc
     5341 gccgtcaaga agggcgacgt ggtaagggcc gtagtggtgc ggaccaagaa agagacgcgc
     5401 cgcgacgacg ggtcgtacat ccgctttggg gagaacgcgg gcgtgatcat caaccccgat
     5461 ggcgcccccc gcgggactcg tatcttcggg cccgtcgcgc gcgagctcag ggagaagggt
     5521 tacaccagga tcatctcgct cgccccggag gtgttgtagt tatgggtctg aaactaaagc
     5581 gcggggacac cgtcgttgtg atctcgggca aagagaaggg caagcgcggc gagatcgaac
     5641 gcgtcatccc gaaagagaac agggtcgtcg tcggcggggt caacgtccgc acgcgccacg
     5701 cgaggccgag ccagcagaat cagcaggggc tctaccactt cgaggctccc atcgacgtct
     5761 ccaacgtgat gctcgtcgac ccgaactcgg gcgagccgac gagggtgggc taccgcttta
     5821 cggactcggg ggagaagctc cgggtctcga aggcctcagg gaaggatatc gatggctagg
     5881 ttgaaggaaa ggtacaatga ggaggtcgtt ccgaatctcc aggagaggtt cggcatcgag
     5941 aaccccatgc ggatgcccgg tctggagaag atcgtggtga acatgggcgt cggagaggcc
     6001 gtggtaaaca gccgcgccct cgacggcgcg atggaggacc tcgccaggat caccggccag
     6061 aaggcccagc tcaggcgggc gcgcaagagc atcgcggggt tcaagatcag ggagggcatg
     6121 ccggtcgggg ccagggtaac gctgcgcggc gagcgcatgt gggagttcct cgaccgcttc
     6181 atctccatag ccctaccgcg cgtcagggac ttcaggggca tcagccccgc ctccttcgac
     6241 gggcgtggaa actacgccgt gggcctcaag gagcaggtca tcttcccaga gatctcctac
     6301 gactccatag acgcgacccg gggtctggac gtggcgatcg tgacgacgac ggagagcgac
     6361 gaagaggcgc gggagctctt gcggctcctc gggatgccgt tcaggcagag ttgagaggta
     6421 ggaaatgacg agaaaggcgc tgctggtaaa gcagaagaag aggcagaagt acccggtcag
     6481 ggagtacaac cgttgccaga agtgtggccg ggcgcacggc tactacagga agttcgggct
     6541 ctgccggatc tgcctcagag aactggcgca cgaggggaag atccccggcg taacgaaagc
     6601 gagctggtga agaatatgtc ggtcaacgat cctatagcgg atttcctgac gcgcatccgc
     6661 aacgcgcaca tggccaagca ggagacggtc gagataccgc actccaagat gaaggcggag
     6721 atctcccgaa tccttctgga ggaaggctac gttcgggacg tcaccgagcg cgggagcggg
     6781 gtcgaaaaga ggctcgtaat agccctgaag tacggccccg acggcagccg cggcatcgcc
     6841 ggcctgaggc ggatgagccg ccccggacgc cgggtctacc gcaagcagac caacatcccc
     6901 cgcgtgctcg acgggctcgg ggtggcgata ctctccacct cccagggaat cctgacagat
     6961 caccaggcca ggcgcagagg cgtcggcggc gaagtccttt gcttcgtcta ctagggaggt
     7021 agcagtgtcg agaatcggga aagcccccgt cgagatcccc ggcgcggtca gcgtggagct
     7081 ctcgggccgc agcgtgaagg tcaacggccc gaagggcgag ctcacggtgc ccgtggccag
     7141 cggtatcgag gtgaggcagg aagacggcaa cgtgatagtc gagcgtgccc aggacacgcc
     7201 cgagcaccgg gcgatgcacg gacttacgcg gtccctgatc tacaacgccg taacgggcgt
     7261 gacggacggg ttcacgaaga cgctgaacat cgccggcgtc ggctacaggg cggccttgca
     7321 ggggagcgac atcaacctgc aggtcggcta ctctcatccc gtctccatct caccgcgcga
     7381 ggggatagag ttcgaggttc cgaacgccac gaccatcatc gtgcgcggga tagacaagca
     7441 gagggtgggc cagacggcgg cggagataag gatggtccgt ccgcccgagc cctacaaggg
     7501 caagggcatc cgctacagcg acgagcagat ccggcgcaag gtcggcaagg ccggatagcg
     7561 aagctaggag gaatcatggc tgttctcgaa aagcggatgc acagggagaa acggcgcaag
     7621 cgcgtcaggc gcaaggtggc cggtacggcc gtaaggcccc gtctctcggt ataccgctcc
     7681 aacgtgcaca tctacgccca gctaatcgac gacgacgcgg gaagaacgct cgccgcggcc
     7741 gactcccgcg aggtaggaga gtcccagaac cgcaaagacg cggcccgcca ggtcggagcc
     7801 ttgatcgcga acagggcctc cgaggccggg atagagacgg tcgtcttcga caggggtggg
     7861 aacaagtacc acggacgcat cgcggcgctc gccgagggcg cgcgcgaggg cgggctcaag
     7921 ctctaacttt ctaggaggaa ctatcttggg acgaccaaga acggataaca gacagggagg
     7981 agggcgcggc cggcaacgcg acggcgcctc ggacctccag gacagggtcg tcgagatccg
     8041 gcgcgtcgcc aaggtcgtca agggcggccg gcgcttcaat ttctcggccc tcgtggtcgt
     8101 cggcgacggc aaaggcaggg tcggcacagg cctcggtaag gccaacaccg tccccgccgc
     8161 catccagaag ggccaggagc gggccaagcg caacatgttc gacgtaccga tgcgcaacac
     8221 gaccatcccg cacgaggtca cgggcaagtt cgagagctct tcggtgctcc tcaagccggc
     8281 ctccgagggt accggcgtta tcgccggcgg cggcgtcagg gccgtcctcg agctcgccgg
     8341 aataaaggac gtgctcacca aggcgctcgg ctcgacgacc tcggtgaacc tcgtgcaggc
     8401 cacggtcgaa ggccttaaga ggctgcgctc caaggccgac atcgagcaga cacgaggggt
     8461 gaagatcacg ctatgagccc cctcaaggtt acgcaggtaa ggagcgtagt gggctccaaa
     8521 cggggtcaca agcggacggt gcgcgccctg ggtctcaagc gcatcaggga ctccagggtg
     8581 caccaggaca cgccccagat ccggggcatg gtcgataaag tgcggcacct cgtgcatgcc
     8641 gaggaggtgg actagcggtg aggttgaacg agataaaggg tgccccgggt tccacgaagg
     8701 cgcgcaagcg cgtcggtcgc ggcacgggct cgggacgcgg caagacttcg ggacgcggcc
     8761 acaagggcgc cggcgcccgc tcgggcgtct ccgcgttcgc ggcctacgag ggcggacaga
     8821 tgccgctgca gcgaaggttg ccgcgcctga agggcgaggc gaggggccgc cacacggtgg
     8881 cccgcccaac agtgtacgcg ccggtgaacc tcggcgaact caaggacctc cccggtgata
     8941 cgataggtcc tgacgagttg cgggccgccg ggctcgtcag gaagaaggct gagctcatca
     9001 agatccttgg ccagggcgat gcggggcgcg ccgttacggt aaaggcgcac gctttctcca
     9061 ggtcggccag ggagaagatt gaggccggcg gcgggagcgt cgaggtactg gagcggtaga
     9121 gatgttggga tccctagcca acgcctggaa aatcccggag ctgcgaatga ggctcctgtt
     9181 cacgttcgcc atgctggcgg cgtaccgcct tggggcgtac atccccctgc ccggggtcga
     9241 cccgagcgta ctggccggcg gactccagag cagcaacccc ataacgggcc tgttcgggac
     9301 gttcacgggc ggagccttca acaacttctc ggtgttctcg ctggggatca tgccctacat
     9361 aacggccgcc atcgtgatgc agctgatgac ggtcgccatc ccacgcctgc aggagctcgc
     9421 caaagaaggc gagacggggc agcagaagat cacgcagtac acccgctact tcacgctggc
     9481 gctggcgctc atccagtcgg tcgcgatggt cttgttcttc cgctcggcgc agggcggagg
     9541 ggcgctcgcg ggtaccagcc ccatcgagat actcgtggcc atcgttaccc tcacggccgg
     9601 cgtgatgctc accatgtggt tcggcgagct cataagccag aggggactcg gcaacgggat
     9661 ctcgctcatc ataacggcct cgatcctctc gcaggtgccg ctggccatta cggcgctcag
     9721 ggagaacggt gacgtgctta ccgtggtcat cctcgggctt ctggccgtca tgatcgtcgc
     9781 cgccatcgtg ttcgtcaacg aaggccagcg caggatcccc ataacgtacg ccaaacggca
     9841 ggtcgggcgc aggatgagcc agggcgggac gacgtatctg cccctcaagg tcaacatggc
     9901 cggggtgatc cccataatct tcgcctcatc gctgttgctc ttccccgtcg tgctggcgca
     9961 actctccgcg gggggagacc ccgactcgat cctcggtcgt ctcgcggcct tcttcgatcc
    10021 cgggaacgcg ccctacctgt tcctctacgc gctgctcatc atcatgttca cctacttcta
    10081 cacggcggtg cagttcaacc ccgtcgagca cgccgataac ctgaagaaga gcggcggcta
    10141 cgtccccggc tacaggccgg gccagcccac ggccatctac ctgaacaacg tgctgaccag
    10201 gatcacgctc ttcggcgccg tgttcctggc cgccatcgcc gtgctgccct acctgatcgc
    10261 cccggcattg ggcctgggga accagctctt cctcggcggc acctccatgc tgatcgtggt
    10321 cggcgtctcg ctggacacgg tgcgccagct ggagagccag ctgatgatgc gcaactacga
    10381 agggttcctg aagaagcgat gaggatcatc ctgcttgggc cccagggagc gggcaaggga
    10441 acccaggcgc agcgcctggc acaggaggtc ggcgccacgc acatctccac cggggacata
    10501 gtgcgcgccg agataaagtc gggctccgag ctcggcaaga aggtgcagga ctacaacgac
    10561 aggggcgagt tggtgcccga cgagatcatc gtcgagatgg ccaagcctta cctcgacgac
    10621 gccgactcct ggctcctcga cggtttcccc cgcaacgagg cccaggcgaa ggccctggat
    10681 caggccctcg aagatatcgg ggaggacctc gacgccgtgg tcgccctcga agcgcccgac
    10741 gacgcgctcg tcgtgcggct ctcggggagg cgccagagcc aggatacggg caagatctac
    10801 cacatcgagt acgatccgcc gcccgagagc gaccctggcc cgttcatcca gcgcgatgac
    10861 gacgccgagg aagccatccg tcgcaggcta cagatctacc acgaccagac cgagcccctg
    10921 aaagactact acgccgaccg cgacctgctc gtcacggtcg acgcccaaca ggagatcccg
    10981 aaggtcacac aggacatcct cggggccgta ggcaaggaag acgcttgatc gtccgcaaga
    11041 gcacctctga gctcgaggcg atgcgtgagg gcgggcgcat aaccgcgggt tgccttgcga
    11101 tgctggagga gaacgtcagg gcgggcgtga cgacgcggga gctcgaccgg ctcgccgagg
    11161 agttcatcca cgagctcggg ggcaagcccg agttcaaggg gtatcagggg ttccctgctt
    11221 ccatctgcgc ctcgccgaac gccatgatcg tgcacggcat cccgggctcc taccgcctga
    11281 agagcggcga cataatctcc ctggacgtcg gcgtccgcta cgagggcttc gtaacggact
    11341 cggcgaccac cgtccccgtg ggatacgtgc ccgaggacat cctcgggctg ctttcgacca
    11401 cgagagagtg tctcgccgcg gcgacggatc agatgcgggc cggcaagagg cttggcgacg
    11461 tgggccacgc catacaggcc ctggccgagt cgcgcggtta cggggtggtg agggacctcg
    11521 tctcccacgg ggtcgggcgc aagatgcacg aggaccccca gatccccaac tacggacgtc
    11581 ccggcaccgg gccgcgcctc ctccccggca tgaccttcgc catagagccc atgatcacgc
    11641 tcggcgatta cggcatacgc ctcagcgaat gggacggatg gtccatctac acggccgacg
    11701 gctccctctc agcccacttc gagaacaccg tcgccgttac cgagaacgga cccctggtgt
    11761 tgacgcagaa tgggagctcg ggcccataga gaacgatgct tttcggtggg agcctccggt
    11821 ccggcgtggt aagatatgcg tttggtctca gccatcttgt aactatttga gaagaggagg
    11881 tccttggcca aagaagacgt catagaggtt gaaggcaccg taaccgaggc gctgcccaac
    11941 acacagttcc gggtcgagtt ggagaacggg cacaacgtgc tggcgcacat ctcgggcaag
    12001 atgcggatga actacatccg gatcttgccg ggcgacaggg tgaaggtgga gttgagcccc
    12061 tacgacttga gccggggacg gatcacttac aggttcagga cataaggaga gagttgtgaa
    12121 agtcagggcg agcgtgaaac cgatatgcga gcggtgcaag gtcataaggc gtcgcggggt
    12181 ggtgcgtgtc atctgcacga accctcgcca caagcagagg cagggctaga gtatggcgcg
    12241 gatcgcagga gtagacctcc ccagggagaa gagggtcgag gtagggctta cctacatcta
    12301 cggcgtgggc cgttcgacgg cgaggaagat ctgccgggaa acccaggtcg atcccgacac
    12361 gaaggtaagg gacctggccg agggcgaggt agccagggtc aggagctaca tagaccagaa
    12421 ctgtgaggtc gagggcgacc tgaggcgcga ggtcaaccag aacatcaggc ggctcataga
    12481 cacagggacc taccgcggga tcaggcaccg caggggcctg cccgtgcgcg ggcagcgaac
    12541 caagacgaac gcccgccagc gcaaggggcc gaagccctcg ataggcggga ggaagaagag
    12601 gtagatgggc aggcagaggc aacagcggca gcgggcgcgc ggcgcgagca gggcgcgccg
    12661 gcgggtccgc aggaacgtaa cgaccggcgt ggtccacata aagagctcct tcaacaacac
    12721 catcgtctcc gtgagcgacc aggagggcaa cgtcatcgcc tgggagtcgg cgggatcttt
    12781 gggtttcaag gggagccgca agagcacgcc tttcgcggcc cagatgacgg ccgagagcgt
    12841 cgcgaacaag gccatggacc agggcgtgag gcgcgtggac atcgaggtca agggccacgg
    12901 ctcgggtagg gacatggccg cccgcacctt ccagtcgatg ggcatagagg tgctctccat
    12961 aaaggacgtg acgggccagc cgcacaacgg atgcaggccg cccaagaggc gcagggggta
    13021 gctaggctat ggcacgttac acaggaccga ggggccggag agaccggcgg gccggggtaa
    13081 tgctctcctc catgcggaag aacccgttgg agaagaagcc ctacccgccg ggagagcacg
    13141 gaaggggcag gcgccgccag accgagttcg gcctcaggct catggagaag cagaaggccc
    13201 gctggtacta cggggtctcc gagaagcagt tcaggcgggc ctacgacagg gcaaccagga
    13261 tgcagggcgt ctccggcgag aacctcctca ggctcatgga gctgaggatg gacaacgtgg
    13321 tctacaggat gggcttctcc accagccgcc cccaggcgcg ccagctcgtc gtgcacgggc
    13381 acttccagct caacggccgc aagcacaacg tcccttcggc catactcaag cccggcgacg
    13441 tcatcacggt caaggagaag agcagggacc tggagcccat ccagaacgcc gtcgagaacg
    13501 tggtcgccgt cccgccctgg ctagaggccg accacgacaa cttcacgggc aggatcctgc
    13561 acactccggg acgcgacgag atcgacgcgc ccgtcgaaga gcagctgatc atcgagttct
    13621 acagccggta gcaaagagac atttctagca ggtgatactt tgacgatgtt ggacatagca
    13681 ccaccccgtt tcagggtgga agaagaagag ggccgccgcg gaaccttcgt agccgagcct
    13741 ttgccgcggg gactcggcca cactctgggc aactcgctca ggcgtgtcat gctctccggg
    13801 ctcacggggg ccgcggtcac caagattcgc atagagggcg tctcgcacga gttctcgacc
    13861 atcgaaggcg tcaaagagga cgtcgtcgac atcatcctca acatcaaggg cttgaagttc
    13921 aagctcgagc gggaggagcc catagagctc accatcgcga aagacggccc cggcgacgtc
    13981 acggcggcgg acatagaact gaaagcagac gtggaggtcg tggatcccga ggcgtacata
    14041 gcgagcgtct ccgacggcgg gcacctcgac atgcgcctga cggtcgagag gggccagggc
    14101 tacgcgaggg ccgagcagaa caagagcgag gccgacccta taggcgtgat cgccgtcgat
    14161 tcgctgttct cgccggtaca gagggtcaac tacatggtct cggagacgcg ggccggcgcg
    14221 agggccgacc tcgactcgct gcgcatggag gtcttcaccg acggacgcat cgggccgcag
    14281 gaggccctgc aggacgcctc caggcagctc atggatttcc tcgggctctt caccgatggc
    14341 taccaggggg cgggcgcccc cgacgcccgc cagcgcggcg gacggcccgt aatcacggac
    14401 gagcgaccgg tagaggacct cgagctaacc gttcgctcct acaactgcct caagcgcgag
    14461 ggcgtcgaca ccataggcca gctcgccacc atgaccgagg aagagctcat gaacatccgt
    14521 aacctcggca tgaagagcgt cgatgagatc cgctccaagc tcctggagta cggatattcc
    14581 ctggagagcg agggcaatga ggcacgctaa acgaggccga aagctcggcc gcgaggcaca
    14641 gcaccgtaac ctcatgctag gcacgatggc gggccaggtc attacgcacg gccgcatcaa
    14701 gacaacggcc ccgaaggcga aagagcttcg cggcgtcgtg gacaaactca tcaacacggc
    14761 caagagggac gacctcgcct ccaggcgcca ggctgtcaag gtactcaagg acaagaccgt
    14821 cgtccgccgc ctcttcgagg atgtggcccc cgagctcgat gaccgtaatt cgggctacac
    14881 ccgtatcctg aagctgggac ccaggctcgg cgacggcgcc gagaccgtct acctggaact
    14941 ggtcaaccac accgtggagt agccaaccct tgcagaaccc aggcgaaggg acccgccccg
    15001 gtgggtcctt cgccgtctct tgcgagagag taaccttctc ctaccccggc tccgcacaac
    15061 ccgcgctctc gggggtgagc ttcgcccttc gccgggggga atacgtcggc gtggtgggcc
    15121 cgaacggcgg cggcaagtcc accctggtcc gcctcttgaa cggtctcctg aagcccgagt
    15181 cgggccgcgt gctggtctcg ggccgcgatc ctgcaacgga acccttcgag gtccgcaagc
    15241 acctcggcgt ccttttccag aacccggaga acggcctcgt cgcccctttc gtcgaggacg
    15301 acgtcgcttt cggcctggag aacctcggcg tcccccggga ggagatgcgc gagagggtcg
    15361 ccagggccgt gcgggccgtc ggcctgcaag gttacgaacg ccgcgagccc cataccctct
    15421 cgggcggcga gaagcagcgc gtcgccctgg ccggtctact tgccgtcgaa cccgagatac
    15481 tcgtcctcga cgaacccacc tccatgctcg acgccgcagg acgcagggag gttctgaagc
    15541 acctcgggga tctacggttc gagaagacgg tcctgcacgt aacccaccac ctcgaagagc
    15601 tcctggacgc cgaccgtatc ctcgtcttga acgggggaga gctcgtggcc gacgaaacac
    15661 ccgaacgact gttctccaac gcggacttcc tgcgggagaa cagcctgctc ctgcccgtcg
    15721 tgccgaggct cgcgctggag ctcggctttc cgccggcgaa gctacgtacg cccgaagagt
    15781 tggccgaggc catactggcc cgtacacaga gcgggacgag gtctctatga agctggagct
    15841 cagggacgtc gggcatgtct acgcacgggg cacccccttc caggtcgagg cgttgcgagg
    15901 cgtctcgctc gtcgtggagc caggagaggt gctcgcgatc gtcggcggca cgggctcggg
    15961 caagagcacg ctagcacagc acatgaatct gctcctcgtt ccgacgagcg gagaggtgct
    16021 cgtcgacgcc gtagatgcga cgacgctcac aaagagcgag ctgcggcgca gggtcggcct
    16081 cgtcttccag ttccccgaat cggcgctctt cgccccgacc gtcgaggagg acgtggcctt
    16141 cgccccacgc cagttggggt tggacgaaga ggaggtaacc gagcgggtgc tcgagtcgct
    16201 gcgggcgctg ggggcggagg aactcgcggc gcgttcacct ttcgcgctct ctggcggcga
    16261 gaagcggcgg gtcgccatcg ccggggtgct cgcgatggga ccagaggtcc tcgtcctgga
    16321 cgagccgacg gccggcctgg atccggccgc cagggaggat cttctggccc tgatcctggg
    16381 cctgcgcgaa gcaggcgtct ccgtcgtcct cgtctcccac gacctggacg aggtcgccga
    16441 agtcgccgac agggtgtgtg cgctccacga gggcagggta agcgccgtcg ggaccgcggc
    16501 cgaggtgttc tacgcagatc ccacacgtgc ccccgcgacg gtacgggtcg cttccctgct
    16561 aaggggggcg agtccaggga tcggacaccc cgtgcgcttt agggagacgc tcgacgcgct
    16621 gcgggcccag caggggacct gagatgacga cccggagcct cggccagttc tatcccgtgt
    16681 cctcgccggt gcacgctctc gacccgaggg ccaagataat cgccacggcc atcttcgtcg
    16741 tcgggctgtt cctcgtagac tcgacggcgg ggcttctctt cgtcgccgcg gcgctcgtgg
    16801 cgctcgtggc ggtgagccgt attccggcag gcaccttctt gcggctcttg cggcccgtgc
    16861 tctatatcgt ggcgctgacg gtcttgttcc agatcttctt ctcgcgggag ggtgttattc
    16921 tcttcgagtg gggcttcctg gaggttcatt ccggcgggtt gaggatgggt ctcttccttg
    16981 ccttgcggat actgttgctc gtctcctcgg cagggttgct cacggcgacg acggcgcccg
    17041 tggcgctcgc tgacgggatc gaggacctac tttcgcccct gaagagggtg cggttcccgg
    17101 cgcacgaggt cgcgatgatg atgaccatcg cgctccgttt catccccacg ctcggggagg
    17161 aggccgagaa gatccaaagg gcccaggccg cccgcggcgc tgacttctcc gagggagggc
    17221 cgcttcgtag ggcgcgctcc ttcctacccg tcctcatacc gctgaccgtc ggcgccttcc
    17281 ggcgcgccga cgagctcgcc gacgcgatgg agagcagggg ctacaggggc ggcgaaggcc
    17341 gcacccgtta ccgcgagctg cgctaccgga tgagcgacgc gatcgcactg gcactcactt
    17401 tgttagtcct gctggtcggg atcgtgcttt gaagctcgcc gggctcgtcg agtacgaagg
    17461 caccggattc gcaggatggg ccgcccaacc agaacgaaga accgtcgagg gtgtactctc
    17521 cggggcgctc tctaccgttc tgcgccagcc ggtcaagatg agcgtcgcag gccgcacaga
    17581 cgcaggggtg catgcgagtg ctcaagtagt ctccttcgag gcggacacag ccctcgggcc
    17641 gtccctgatc tcctacaaga ccacggcggt actgcccaaa gacgtcgcac tccgtcgctg
    17701 catagcggtt cctgacgagt tcgatgcccg tgccgacgcg aggagccgga cgtacgagta
    17761 caggttggtc aacgatgaga tcaggtcccc tctggagcgc catcacgcgg gctacgcgcc
    17821 gcagagactc gacctggaac ttctttccga agcggcaagt ctggtggaag ggacccacga
    17881 cttcagggcc ttcacccccc agaagagtta ccacgtccgc ttcgaacgaa tcgttaccgg
    17941 ctcgcgctgg accaggcaag gcgacctgct aaggtatcgc ataaccgccg actcgtttct
    18001 gtacggtatg gtccgtaccc tggtcggcac catgctcgag gttgcaggcg gaaagagaga
    18061 cttgccggag ttcgaacggc tgctctcggg aggaaaacgc agtgaagcag ggcccgccgt
    18121 tggcagccgc gggctcacgc tggtcggcgt gggctacgac gacccgaacc tatggaggag
    18181 tgagcgatga acacgggcga tacgagagag ctgatgcagg agatcgacac ctacgtcgag
    18241 gacctgttta ccccatcgga cgagatcctc gaagcagcct tgcgggactc caggcgggcc
    18301 gggctgcccg agataaacgt ctcgcccaac cagggtaggc tgctgcaact gcttaccggg
    18361 atagccgggg cgcggcgcat actggagata ggcaccctcg gcggctacag taccatccac
    18421 tttgcccgtg cccttcccga cgacggagcc ctgatctccc tggaaatcga cgagcatcac
    18481 gccggggtcg cccgcaacaa catcgaacgt gccagcctct ccgagaaggt cgagatccgg
    18541 gtcggcgacg cccacgaact cctcgcctcg ctcatcgaag acgaagaagg ccccttcgac
    18601 gtaatcttca tagacgccga caaagagagc taccccgagt acctggacgc gtccctacgc
    18661 ctcgtacgac caggctccct gatcctcggg gataacacca taaggggcgg ctccgtactc
    18721 gaccccaaaa actcagcggc ccgggcgacg cgcgagttca acgagcgcat agcaagagac
    18781 ccaaggctct tcagcatcgc attgcccctg attcgagagc acatagacgg actcacgata
    18841 gcaagggtcc tgtaagagca gcttgcaaag tggttgaacc tctttgcaag ctctcagcaa
    18901 gtcagccggt caacatttca gcctcaacca aagagctgac aagctgaagt gctgacaagc
    18961 gcccgccagg gcgcgtgctg accagctgct ttacgctggt ccttttgccg atgtatgcta
    19021 ctatttgatc cgtctaaagg cctttgaagt tcgcacaagc tccggaggat gatgaagagt
    19081 tacatggcgc ggccgctgga ggtcgagcgt aagtggtatg tcgtcgacgc ggagggaaag
    19141 cacctcgggc gtctggcgac cgagatcgtg cgcgttttgc gcggcaagaa caagccgcag
    19201 tacacgccgc acgtcgatgt gggcgacttc gtcgtcgtgg tgaacgcgga cagggttgcc
    19261 gtcacgggca ggaaggccga gcagagggtc tacaggcggc actcgggcta tcccggcggt
    19321 atgaaagaga ccagctacga gcagatgctc gcgcgcaagc cgaccgaggt cctgcgcaag
    19381 gccgtctacg ggatgatgcc caagacccgc ctcgcccgta agcagttcaa gaagttgaag
    19441 atctacgcgg gacccgagca cccacactcg gcccaggacc cgcagacgct cgaggtgacc
    19501 taaatggcag aggctctata taacgggacg ggaagacgca agaccgccgt agcaagggta
    19561 aggatactcc ctggcgacgg taagatcacc gtgaacggcc gtgacgtcga cgagtacttc
    19621 ccgcggccgg cctaccgaac ggcggtacgc gcgccgctcg agctcctcgg ttcggccggc
    19681 cgctacgagg tcacagccaa gatcgaaggc ggtggcccca ccgggcaggc ggaggccttg
    19741 cgccacggta tagcgcgggc cctcgccgaa gaatcgcagg atgcccgcag cgagctgaaa
    19801 gcggccggga tgctcacccg cgactccaga gccgtcgagc gcaagaagta cggcctcaag
    19861 aaggcccgca agcgcccgca gttctccaag cgttagagtc cccagagcgg atcttcatgt
    19921 cgggctcctt tcggcgtgcg aaaggggccc gttctacgtc gagaaggaga ggtgttaggg
    19981 cttcgggatc taccccggca tggcaaggga ggccgactga atagtgggcc catatcgttc
    20041 ggcaccgacg gggtgcgcgg ggtcgccaac aagggcctgc tccccgaatt cgccctgagt
    20101 ctcggactcg ccgcctcccg cgcctttggg ggcaggatcc tcgtgggacg cgacacgcgc
    20161 ctctccggag agatgctcgg cagcgccctg tgtgccggcg ccgcaagcgg cggcgcagac
    20221 gtcgtagatc tcggcgtcct tcctacgcca ggcgtcgcgg ccctcgcacc gcgtctcggg
    20281 gccacggccg cgggcgtcgt aagcgcctcc cacaatccct atcccgacaa cggcatcaag
    20341 ttcttctctg gagaaggtcg caagctctct cctgagaaag aacgtgagat cgaagacctg
    20401 ctgggcagcg acattacacg cccgaccggg agcggtgtcg ggagggtcga ggtactcggc
    20461 gacgccacgc gcatgtacgc ccagtgggta ctccagcggt tgcgtccgcc ggctgacggt
    20521 gtcaaggtac ttctggactg cgccaatggg gctgcctacg aggtggcgcc gctggtcttc
    20581 ggagagaccg gcgcggatct caccctgacc ggagtcgatc ctacgggtac caacataaac
    20641 gag
//
LOCUS       DATMVF010000212        17905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_490625, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000212 DATMVF010000000
VERSION     DATMVF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 17905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17905
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490625"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(243..323)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            808..2064
                     /locus_tag="VN178_10715"
     CDS             808..2064
                     /locus_tag="VN178_10715"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HWS81485.1"
                     /translation="MLDAIEIKKVGGVLPEPNIREVVEGYSMGKVHDYQMASLLMAIF
                     IQGMSYEETLALTRAMAESGEAYSFPECVDKHSTGGVGDKVSLTALPIVAACGAPVAK
                     LSGRGLGVSGGTIDKLESIPGFSCNLSKEQFSNQVEEIGLAIIEAGDLAPADREIYAL
                     RDTTGTVDSLPLIGSSIVSKKVATGAGHLLYDVKRGSGAFMKTTEEARELAGLLVRLS
                     EDLGIDATALITDMHDPLGSAVGNALEVRESVLLLKNEPVPDDLASEVDHVATRLLEL
                     KGIPKAAAAVENALSSGAAYEKFREFVGAQGGDADALEDLPVSAKVSEVRSPRSGYVA
                     RFGASAIGRAALALGAGRKHKGDRIDPGSGVEVLVKPGDPLQQGQPVARLYGEREAEP
                     ARRLVLEALEISDEPVDPPPVILDSL"
     gene            2241..2954
                     /locus_tag="VN178_10720"
     CDS             2241..2954
                     /locus_tag="VN178_10720"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HWS81486.1"
                     /translation="MCPVHVRAEPGDFAKSVLLPGDPLRAKYIAETFFEDAKLVTEER
                     GMLGYTGTYRGEPVSVQATGMGCPSAAIVTEELIQLGVENLLRVGTCGGFSRELRLGD
                     LIVATSAAPQDGTVSSITRGVPYAPAAHFDIVHAAHHAAESAGRRTFVGQVVSSDLFY
                     DPTEDPARLWGDLGVLAVEMEAAAIFTIAAMRGVRAGCLLTVSDTIGAEIVRISDDEL
                     RNAVDNMMALALDTLNAVN"
     gene            3018..4349
                     /locus_tag="VN178_10725"
     CDS             3018..4349
                     /locus_tag="VN178_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HWS81487.1"
                     /translation="MTILNESGIPDVLPPLHAVNDLAQRLPDALIVVAGTQAEAHLVQ
                     SLPAVPSPNLPPFRRNQRVIFAVLDPDDPPQQGLLASRIVESAAGFRGTRLVLLVAGR
                     AAEALGIDAEFETRLVARKLDLPAEVVNPDAPREVPGILSTDLEDDVLAAMVNLCPKR
                     KTSDLVETASPAKRGGLFGGFLRRGTETSHSGGGRPVVLLGGALSSGDARVLAAELSR
                     AGVEVAGSVPGSGIRELPPLGEGTVVGVMDPNLTSAATAAERRGATVVKTLMPVGVDG
                     TARFVQDVAEEAGSRTSEVVRARSAWEDLGVLRNRIRGKRIFFTGDTGFEIPLARFLA
                     DAGAVVLEVGAPRLDRRFLAAELQALGPDVDVVVSPDYRGQTNRIDESRPDVVISSPG
                     LHLPLVARGHLCRSPRDFLEAGPYGYGGARRILELLVRTMERAEALDSLNL"
     gene            4346..5776
                     /locus_tag="VN178_10730"
     CDS             4346..5776
                     /locus_tag="VN178_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HWS81488.1"
                     /translation="MKLLRGVYEGPQSYGIVRVAASIGGVHTLLRALPGEAYFRTLYG
                     ARERTGAMAPVTLSPMREGPDHSMAPGNLTRDLSSVVRRHPKAEIVILARSEAALLSG
                     EAIPTTLPQVEGTGIPPQLVTFPWEQPGMRETEAAELALEDLIHAGMHHRPERSPYPT
                     VNIFGPPIFGPGAAAEYAEAERLLDMIGVEVNARVPLGANARDLGRLQRAWANVLLYR
                     EIGESATLYLQDEFGMPRVTTPMIGAAGTGAVLRSVGELCQLDPKSVQRTIWAELAST
                     AKLPWYARLEPPETFQNRRVAIFGDFTYPIGLGYALAREVGLEIIACGTYLTHLERDF
                     RFHAHTFTEGAFVEDDPDRVATRIEAAKPDLVVGTHLEEGVADSLGVPFLPLCPPVSD
                     TPFVVRPLMGYAGSSVLADTLDKVLRENGREPAGEAGMQWTTEALQEAAAVPLFLRAR
                     ARRLAEEHARSLGSAKVTREIFLESR"
     gene            5885..6304
                     /locus_tag="VN178_10735"
     CDS             5885..6304
                     /locus_tag="VN178_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81489.1"
                     /translation="MRKSLLLALVSCVLLLAGYFGTAGADPLNNNRLQIPASCKEDGE
                     QFTFVINGEGIAGHIIGSTSNIHPVEDTVTYTYRDPTTGKRVKETVIDSLGSGEKTGL
                     QSDLKRCSGTVSYEDPELGLVTADFDILAYFTPRGGS"
     gene            complement(6337..7533)
                     /locus_tag="VN178_10740"
     CDS             complement(6337..7533)
                     /locus_tag="VN178_10740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81490.1"
                     /translation="MGQTVSNLGSSFTVFTLPLLVFKLTGSALSLAYITAAEYLPYLI
                     FGLIIGAGVDRVDRRRLMIFVDTVQALVIFTIPLLAVLDLLTLWWVYAVGFVSSTLWV
                     CFNTAEFAALPSLVSKDDLVAANGRLQASYSAATVAGPLLAGLLMALVPVYTVLVLDA
                     FSFLVSVLCLTLIRSSFNADGPGRRESGSLRRDIGEGLRCVLGHPVLRSVAIMMALVN
                     CVGFTVYAQLVLFAKERLGATDSQVGFLYAAGSVGMIALALAADPLRRRLPFSKVVLG
                     TLMLGGVAIVALASTRWYWGALVLWALIWGLVILVQINSNSLWQTIVPDRLLGRVQGV
                     ISVLSWSAIPLGAFVGGVAIEQTQQVALVYGAIGATIFLIGLLFSFTALGRAEQYLPA
                     QQPTER"
     gene            complement(7900..9759)
                     /locus_tag="VN178_10745"
     CDS             complement(7900..9759)
                     /locus_tag="VN178_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWS81491.1"
                     /translation="MLKIYTRDSEVRRKGRILAVFILGILGSGIGLAGFNLIQGSHEQ
                     NISNGVFLSLIVGLFMLNRLGYVRIAAILTTTTMTVGPVVFLSQQDLAETYIVMCVPV
                     FVASFLIVPWAGLAVGAIVVLGTLVLGDASITYLSLFVFATLSLIVYFVANTVDSAYR
                     ENRYRALHDSLTDLPNRSLFTNRLAEAVERSSKQRLSYGVLFIDLDNFKVINDSLGHT
                     AGDELLVVIARRLLSCLRPADTPARLSGDEFAVVLDGIADIDDVIKVSDRIHKVVQAP
                     VQLGVRQVYISTSIGIALGKANEADPTTLLRNADVALYEAKKEGKGRSRVFDWSMYSR
                     ARKRLELENELRLAIEKEELTLYYQPMVRLSTGKVSCMEALVRWEHPERGLIYPEEFI
                     PLAEETDLIIPLGHWVMQNACRQAREWTRLSSDGSPLVLSVNVSVKQFQHPDFVGAVE
                     KILRDTGLEVSRLQLEITETVVTENVEYAAGLLKDLKRLGVQLAIDDFGQGYSSLGAL
                     RTFSLDNLKIDKSFVAGLGENAQDTSIVQLVIDLAHSLDMKAIAEGVESAEQLLLLQQ
                     MGSDLAQGYYFAKPLTVEAATALFADSPQWLVRRRDLLDRGQLGPFLDARRLP"
     gene            complement(9936..11471)
                     /locus_tag="VN178_10750"
     CDS             complement(9936..11471)
                     /locus_tag="VN178_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HWS81492.1"
                     /translation="MVIKGYAHAARLSRYLPFAVSELLRTDLSLLTRPTDYDSSWAAY
                     LVEEDGSPSYPDVLPTLLERQHSDGGWGGRVSHSHDRLLTTLAVVLLLARLGNRRRDK
                     ERQAVGEHYIWRVASELDHDGQATVGFELLLPALLELGKRMGANLPYSHLRRYETTRV
                     KKLNLLPSERIFQSTTTALFSLEAFVDDIDLNDATSLLQQDGSMASSPSATAALLGQA
                     ADWRVRFPRSTGYLENLLGRYDGGLPAIAPCGIFTRAWVLHYLQHGRLLSGYESLLRP
                     HYRYLRKHLTAEGVGFSPHVFPDADDTAVTLFVLHNAGYDVDGSCLLQYERDDHFAVY
                     PGELDPSISANLHILEALETLPAAARQRTREKVIGYVLDARQPGGYWEDKWHASPYYP
                     TSQAVMALLPHAPGRMEVTLDWLLSTQHTDGSWGQYGPTVEETALSLLALLSYYRANR
                     RVAQERLRLAARYLIANEMPFKDDYPELWIAKALYAPTFVIRSIVLAALGMFLDTFGD
                     KGL"
     gene            11707..12561
                     /locus_tag="VN178_10755"
     CDS             11707..12561
                     /locus_tag="VN178_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81493.1"
                     /translation="MSRPRPVVMGWPFNGTRRWYLLQRGRHPNTDDYVVTLIRRQAEL
                     HRLIFAHGISVIMAPGFGSELLARGTDYTRLVLGGLLRLVEDPVYQEMFSSGTRIRFY
                     GDFQEVLDTPAFRPMLRACADLTTATASGDGPLLLIGLFADAPYPTLARLSVEFATRR
                     GRPPDRRELIEAYYGLDVPDLSIYLGFAQPSVFDVPLVATGLEDLYATLTPSPDLTEA
                     QLRRILYDHLVTRRTPTPDYGTLSADARAALTEYNERHSGITLGVGRIDPLTKVWTPL
                     LPDNTSDG"
     gene            12687..12974
                     /locus_tag="VN178_10760"
     CDS             12687..12974
                     /locus_tag="VN178_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81494.1"
                     /translation="MGIRDFIQYLKEHQERLKSGPELSDVEREETLREAETMVGMADA
                     AIRELESVLGNRRVNLPEEHAVRFAQDVFLYLREEEEKVKAALSAPASRDG"
     gene            complement(12988..13347)
                     /locus_tag="VN178_10765"
     CDS             complement(12988..13347)
                     /locus_tag="VN178_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81495.1"
                     /translation="MYPKGMDASPNKREVPVDLARAGEARYFITWRGAVAGLGGGRLN
                     ELAVAAEAVVTDVLLSAREGVLEIESVDEEEVVSITIEHPEIKQRRLKGLGRVLERFL
                     DDYEITPTRAVLVKRLR"
     gene            13432..14532
                     /locus_tag="VN178_10770"
     CDS             13432..14532
                     /locus_tag="VN178_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWS81496.1"
                     /translation="MKSRSTGISASLAFMLGTILLMGCSEKEPGVQADNTAKASPQAS
                     ILADEPVARVASKVGPSVVQVNVRAVRETPLGTQRGEGLGSGVIYRPDGYIVTNNHVI
                     EGATGVNVAFADGTIEVASIVGTDPNTEIAVIKVDRNGLPAATFDDASTPVVGQLAVA
                     IGSPSGFESTVTSGVVSGVGREFPPELTIGGTAERSALTDLIQTDAAISPGNSGGALA
                     DRDGEIIGINVAYLPPAETGAVDLGFAVPSDTAVSVADQLIEDGEVTTPYLGVLTTDL
                     SPEDAGRFDLPIDSGALVERVVPGSAAWRAGVRRGDIITALGDARIVGYGDLLGALRD
                     HEPGERVTLTVFRNGEERKLAVTLGQMSQQGG"
     gene            14538..14774
                     /locus_tag="VN178_10775"
     CDS             14538..14774
                     /locus_tag="VN178_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81497.1"
                     /translation="MQGVWVRRLMETFAILTLGDGIIAVIFPTEHSLLWEFGPARVRK
                     IARFFAENPNLMRLLGAAQVAFGIRLAKRQYHGR"
     gene            14825..15748
                     /locus_tag="VN178_10780"
     CDS             14825..15748
                     /locus_tag="VN178_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWS81498.1"
                     /translation="MGHHEHDHPANARGGNRRALAVVLVLTASYTAVEIIGGILTGSL
                     ALLADAGHMLSDNFSLGVALFAAWLAGRPATPERSFGYRRAEILAALANGMTLVAISI
                     WVFVEAYSRFREPAEVLGAPMLAIAALGLLVNAVGAAILYRTGGENLNVEGALRHVLA
                     DALGSVGAMVAAATIILTGWRYADPLVSVAIGLLILGSSWTLLRDSTNILLEATPRGL
                     NAGEVGRRMAAAEGIVEVHDLHIWTITSGFPALSAHVLVGRHEDCHSRRRELEELLTH
                     EYGISHTTLQVDHVGDHHTSLQTLRFPSRPD"
     gene            15884..16264
                     /locus_tag="VN178_10785"
     CDS             15884..16264
                     /locus_tag="VN178_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HWS81499.1"
                     /translation="MSKIGRLKRWAQRLRVEIYALYLVYKDPRVPWYARAFAAVVVAY
                     AFSPIDLIPDPIPVLGYLDDLVIIPLGVALAIKMVPPRVLAECREEARGVEDRPVGKV
                     AAVVVVAVWISLAALAVWLVARVL"
     gene            16328..16918
                     /locus_tag="VN178_10790"
     CDS             16328..16918
                     /locus_tag="VN178_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HWS81500.1"
                     /translation="MAKSSRRSRSSESGGSRRAYLIVGAVIVAFVAGFIALAVLDARQ
                     RSGGATGDVKTYDVGQAGEHTQGEVDYAQNPPAGGEHNPVWQNCGFYGKPVRDESAVH
                     SLEHGAVWITYSPDLPQDQIDELRGIAESQSFILVSPFPDLPSNTPVVASAWGKQVGL
                     SGSDDPDLENFIQAYQQGPQTPEPGAVCTGGTSATL"
     gene            complement(17229..>17905)
                     /locus_tag="VN178_10795"
     CDS             complement(17229..>17905)
                     /locus_tag="VN178_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS81501.1"
                     /translation="GEERRGYMSEPITVLLVDDHALVRQGVRAFLQTQDDISVVAEAE
                     SGEEAVRLAAEHAPDVALMDLIMPGMDGVETTRRLVASSPRTNVVMLTSYHDDEHIFP
                     AIRAGALSYVLKEIGPDELAEAVRKAASGEAVLHPRVAARVVRELHGARRAEPNVFHD
                     LSERELEVLKLIAEGLHNSEIAGRLYVSEKTVKSHVSNILSKLHLADRTQAAVYAWRE
                     GVVRRD"
BASE COUNT         3435 a         5671 c         5591 g         3208 t
ORIGIN      
        1 cgctatttca gcgccgggtc cttggcgaag ctggatcgaa gggagaagca gctggccgaa
       61 cagccgcaac tggtggcagg ataacgagcg acgaggagtg gagcggcacg ggactcttac
      121 acacttgaca ggacgcatac cacgacgagc ttccggccac tgccccagcg acgccctcct
      181 atgcttctta tgtactcttg gggcgtcctg gaccgtgcat ccgtcattac ctttgtgctc
      241 ctttcggcaa cccggctgtc ccttgtgtga agggacctag ggctttgcgc ccccgcctta
      301 cgacgagttt gccttttcgt gggcagttgc tatagcttct ctacggaaac cctataaatc
      361 gtcctcccag ttgggtctgt agtcctcgta tctgtgccct gactcccatc tagagtgtcg
      421 ttgccgctaa gtgcgactcg ggagtcgagc gggtcgttgt tatgctgccc tcacggcgtc
      481 ccggcttaag taacacctgc accatacatc ccgaagaagg ggcggcgtat cactcaattg
      541 cagtattttt tgccttcact gtgaatattt tcggtacctt cccctccact acatcgtgat
      601 gccctgtatt ctttcatccg gggatccaga gcaaccatcc acattttcta gacccacggc
      661 aagagacctg gcgaaagtcc ggaccctcat tcgcagaccc accgggttta tgggatgttc
      721 ggtcggcgag cggacgttaa tatggtcgaa atcctgaaac cgacggtacg ctcatgaagg
      781 gggaggaaat tgtctttcgg gagcgcagtg ctcgatgcta tagagatcaa gaaggtcggt
      841 ggagtactac cggagcctaa tatccgggag gtcgtggaag gctacagcat ggggaaggtg
      901 catgactacc agatggcctc gctgctgatg gctatattca tccaggggat gagctatgag
      961 gagacgctcg cgctgacgcg ggcgatggcc gaatccggcg aggcttactc cttccccgag
     1021 tgcgtggaca agcactctac gggcggcgtc ggggacaaag tgagcctcac ggcgctaccc
     1081 atcgttgccg cctgcggggc tcccgtagcc aagctctcgg ggcggggcct cggcgtgtcg
     1141 ggcggcacga tagacaagct ggagtctatc cccggcttct cgtgcaacct ctccaaggag
     1201 cagttcagta accaggtcga ggagatcggg ctcgccataa tcgaggcggg cgacctcgcg
     1261 ccggcggaca gggagatcta cgccctccgc gacacgacgg gtacggtaga ctccctgccg
     1321 cttatcggct cctctatagt ctccaagaag gtcgccacgg gcgccgggca cctgctctac
     1381 gacgtgaagc gcggctcggg agctttcatg aagacgaccg aagaagcccg cgaactggcc
     1441 gggctactcg tacgcctgag cgaggacctg ggcatcgacg ctacggccct gatcacggac
     1501 atgcacgatc ctctggggag cgccgtcggt aacgccctgg aagtccgcga gagcgtcctt
     1561 ctactcaaga acgagcccgt ccccgacgat ctggccagcg aagtagatca cgtcgccacc
     1621 cgtctgctgg agctaaaggg catcccgaaa gcggccgccg ccgtcgaaaa cgctctctcc
     1681 tcaggcgctg cctacgagaa gttccgcgag ttcgtcggcg cccagggcgg cgacgcagac
     1741 gccctcgaag acctccccgt ctccgccaaa gtaagcgaag taagatcccc gcgcagcggc
     1801 tacgtggccc gcttcggggc ctcggccatc ggccgagcag ccctcgccct cggggccggc
     1861 cgcaagcata agggcgaccg gatcgatccc ggctccggag tcgaagtcct cgtcaagcca
     1921 ggagaccctc tccaacaggg ccaacccgta gcccgtctct acggcgaacg cgaagcggag
     1981 ccggcacggc gcctcgtact cgaggccctg gagatctccg acgagcccgt agatcctccc
     2041 cctgttatcc tggatagcct gtgaaatagc tggtcagcat gtcagcatgc cgccaggctt
     2101 cgccaggcgg ctcgtcagct agtcagcaaa agaaaccttg acaagctgaa atgctgacaa
     2161 gcgacagccg caggctggag cgtgctgaca aggcgcgaag cgccgtgctg aagtgctgag
     2221 acgaccgaag ggagtcgacc atgtgccctg ttcatgtgcg cgcggagcct ggagatttcg
     2281 caaagagcgt tctgttgccc ggcgacccct tgcgggccaa gtatatcgcc gagaccttct
     2341 tcgaggacgc caagctcgtg accgaagagc gtgggatgct cgggtataca gggacctata
     2401 ggggcgagcc ggtctcggtc caggccacgg gcatggggtg ccctagcgcc gcaatcgtga
     2461 cggaggaact gatacagcta ggggtagaga atctactcag ggtcggcacc tgcggagggt
     2521 tcagcaggga gttacggctg ggggatctca tcgtcgctac ctcggcagcc ccgcaggacg
     2581 ggacggtctc ctcgatcacc cgcggcgtcc cttacgcccc ggcggcccac ttcgacatcg
     2641 tccacgccgc ccaccacgcg gcagagagcg cggggcgaag gactttcgtc ggccaggtag
     2701 tcagttccga cctgttctac gaccctaccg aagaccctgc gagactgtgg ggtgatctcg
     2761 gggttctcgc cgtcgagatg gaggccgcgg cgatcttcac catagccgcg atgcgtggcg
     2821 tgagggcggg atgcctgctc accgtctccg acaccatagg cgccgagata gtcaggatca
     2881 gcgacgatga actgcgtaac gccgtggaca acatgatggc actggcgctg gataccctaa
     2941 acgccgtaaa ctagtatctc ccgtggggac gagatcgagc aggtcaggcg ccggcttctg
     3001 ctagatagga aagtagagtg acgatcctca atgagtcggg gataccggat gttctccccc
     3061 ctttgcatgc cgtaaatgac ctggctcaga ggcttcccga cgccctgatc gtcgtcgccg
     3121 gaacgcaggc cgaggcgcac ctcgtgcaga gccttccggc cgtaccctcc ccgaacctac
     3181 cacccttcag gcgcaaccag cgggtgatct tcgcggtcct cgaccccgac gacccgccgc
     3241 agcagggact tcttgcctcc cgaatagtgg agagcgccgc cgggttccgt ggcacgagac
     3301 tcgtgctgct cgtggcggga cgggcggcgg aagcgctcgg gatcgacgcc gagttcgaga
     3361 ccaggctcgt ggcccgcaag ctcgacctgc cggccgaagt ggtgaatccc gatgctccac
     3421 gcgaagtgcc tggcatcctc tccaccgacc tcgaagacga tgtcctcgcc gccatggtga
     3481 acctctgtcc gaagaggaag acctcggacc tcgtggagac cgcctctccg gcgaagcgcg
     3541 gcgggctctt cggcggcttt ctgagacggg gcaccgagac ctcgcactcc ggtggtggcc
     3601 gccccgtagt gctgctcggt ggcgcgctct catctggaga cgcgcgggtt ctcgccgcgg
     3661 agctctccag ggccggggtc gaggtggcag ggagcgtacc cgggtcaggg ataagggagc
     3721 ttccgcccct cggggaaggt acggtggtgg gcgtgatgga cccgaacctg acctcggcgg
     3781 caacggccgc cgagagacgc ggggccacgg tagtcaagac cctcatgccc gtcggggtcg
     3841 acggcacggc ccgcttcgtc caggacgtgg cggaggaagc ggggtcgcgg accagcgagg
     3901 tcgtgcgggc caggtctgcc tgggaggacc tgggtgtcct gcggaaccgc atcaggggaa
     3961 agaggatctt cttcaccggc gacacgggat tcgagatccc gctggcccgc ttcctcgccg
     4021 acgcgggagc cgtcgtgctc gaggtcgggg cgccgcggct cgaccgcagg ttcctcgccg
     4081 ccgaactgca ggctctcggt cctgacgtcg acgtcgtcgt ctcgccggac tacaggggtc
     4141 agacgaatcg tatcgacgag tcgcggcccg acgtggtgat ctccagcccc ggcctccacc
     4201 tgcctctggt cgcccgcggg cacctgtgcc gctcgccccg tgatttcctc gaggccggac
     4261 cctacggtta cgggggcgcc cgtcgcatcc tcgagctcct ggtgcgcacc atggagcgcg
     4321 cagaggccct cgactcactg aacctgtgaa gctcctgcgt ggcgtctacg agggaccgca
     4381 gagctacggt atagtgcgcg tggccgcgag catcgggggc gtacacacct tgctcagggc
     4441 tctacctggc gaagcgtact tccgtacgct ctacggggca agggagcgaa ccggggcgat
     4501 ggcgccggtc acgctcagcc caatgcgcga gggacccgac cactcgatgg ccccagggaa
     4561 cctcacccgc gacctctcta gtgtcgtccg ccgtcacccg aaggcggaga tcgttatcct
     4621 ggcccgctcg gaagcggccc tgctctctgg ggaggccatt cctactactc ttccccaggt
     4681 agaaggaaca ggaatacccc cccagttagt gacgttcccc tgggagcaac cgggcatgag
     4741 agagaccgag gccgcggagc tcgcgctgga ggacctgatc cacgccggga tgcaccacag
     4801 gcccgagagg agcccttacc ctacggtcaa catcttcggg ccaccgatct tcgggccggg
     4861 cgcggcggcc gagtacgccg aggccgagcg gctcctagat atgatcgggg tcgaggtaaa
     4921 cgccagggtg cctttgggag cgaacgcgag agacctgggc cgcctccagc gcgcgtgggc
     4981 caacgtgctc ctctacaggg agatcgggga gtccgcgacg ctctacctgc aagacgagtt
     5041 cgggatgcca cgggtaacga ccccgatgat cggcgccgcg ggaaccggcg cggtcttgcg
     5101 gtccgtaggc gagctgtgcc agctcgaccc gaagtccgtg cagcgtacga tctgggccga
     5161 gcttgccagc acggcgaagc tgccctggta cgcgcgcctc gaaccgcccg agaccttcca
     5221 gaaccgcagg gtcgcaatat tcggggactt cacctatcct atcggactcg gttacgccct
     5281 ggcccgcgag gtagggctgg agatcatcgc ctgcgggacc tatctgactc atctcgaaag
     5341 ggacttccgc ttccacgccc acaccttcac ggaaggggct tttgtagagg acgatcccga
     5401 tcgggtcgca acccgcatcg aagccgcgaa gcccgacctc gtcgtcggga cgcacctgga
     5461 agaaggcgtt gccgattccc tcggagtacc cttcctcccc ctctgtcctc cggtatccga
     5521 cacgcccttt gtcgtacgac cactgatggg ctacgccggg tcgagcgtcc tcgccgacac
     5581 gctcgacaaa gtcctgcggg agaacgggag ggagccagcg ggagaggccg gcatgcaatg
     5641 gaccactgag gcgctacagg aggcagcggc ggtaccgctg ttcctgcggg cccgggcccg
     5701 tcggctcgcc gaggaacacg cgcgcagcct gggctcggct aaggtgacgc gagagatctt
     5761 cctcgagtct cggtgagcgg accagtgtag ggaccaccgc cgtcccgcgg gcgcatcatc
     5821 gcgctgtgcg tgatcgctgc gtccgctaat attcatgagg tacttcaacg ttaaggagtg
     5881 aaaagtgagg aaatcgcttc tgctcgcgct cgtttcatgc gtgttacttc tggctggtta
     5941 cttcggcacg gccggtgcag atccgctcaa caacaaccgt ctgcagattc cggcctcctg
     6001 caaggaggac ggcgaacaat tcacgttcgt tatcaatggg gaagggatcg ctggccacat
     6061 aatcgggagc acgagtaaca tccaccccgt ggaggatacg gttacgtaca cgtataggga
     6121 cccgacgacc ggaaagcggg tgaaagaaac ggtgatcgac tccctcggta gtggtgagaa
     6181 gaccggactt cagagcgatc tgaagcggtg cagcggaacg gtttcctacg aggaccccga
     6241 attggggctg gtcacagccg atttcgacat actggcatac tttacacctc gtggtggaag
     6301 ctaacgccac tgaagccgta aacgttccgt cgggtctcaa cgctctgtcg gctgctgcgc
     6361 cggcaggtat tgctcggcgc ggcccagggc cgtgaaggaa aagagaaggc cgataagaaa
     6421 gatcgtcgcg ccgatcgccc cgtacaccaa agccacctgc tgcgtctgct cgatcgccac
     6481 gccgccgacg aaggcaccca gaggtatagc cgaccacgac agcaccgaga tcacgccctg
     6541 caccctaccc agtaaccgat ccggtacgat cgtctgccag aggctgttgc tgtttatctg
     6601 gaccaggatc acgagccccc agatcaacgc ccagagcacc agggcgcccc agtaccaccg
     6661 cgtcgatgcg agcgcaacga tagcaacccc cccgagcatc agggtaccca acaccacctt
     6721 gctgaacggc aggcgtctgc gcagcgggtc tgccgcaagg gccagcgcga tcatccccac
     6781 gctcccggcc gcgtagagga agcccacctg gctgtccgtg gctcccaaac gctcttttgc
     6841 gaagagaacg agttgggcgt agacggtgaa ccccacgcag ttcaccaggg ccatcatgat
     6901 ggcaacgctg cgcagtaccg gatgacccag tacgcagcgc agtccctcgc caatatcccg
     6961 tcgcaggctc ccggattccc gccgccccgg accgtcggcg ttgaaactcg acctgataag
     7021 cgtcaagcac aggacagaga ccaggaatga gaaggcgtcg aggacaagca ccgtatacac
     7081 cggcaccaga gccatcagca ggcccgccag caacgggccc gccaccgtcg ccgcagagta
     7141 gcttgcctgg aggcgcccgt tggccgccac gaggtcatcc ttgctgacca ggcttggcaa
     7201 cgccgcgaac tcggcggtgt tgaaacagac ccacagcgtc gagctgacga accccaccgc
     7261 gtacacccac cacaaagtca gcagatccag cacggcaagc aatgggatag tgaagatgac
     7321 gagggcctgg accgtgtcga caaagatcat aagccgcctg cgatctaccc ggtcgacgcc
     7381 cgcgcctatg atgagaccaa aaatcagata tggcaagtat tcagcggcgg tgatgtacgc
     7441 caggctcaag gccgagccag tgagcttgaa gaccagcaat ggtaaggtga acacggtgaa
     7501 ggagctaccg aggttcgaaa ccgtctgccc cacgaggaac ttccagaaat ccggggcgaa
     7561 tctgcccgcg cggcgagtat ctccaggacc aggtgccgcg gactcccgca tggccgacct
     7621 ttaggctgtt acgggataga tgctgtgctg accggcggcg agcaggaagc acctgccaag
     7681 ctgtcttcgt gcgggttcac ctctgctcac gcgtaatcct ccttacagcg cggtgcataa
     7741 gggccctacc cttatgcacg acgagctatt ggctttcagc tttcagcaag tacaaaaagc
     7801 tgaaggctga aagctgagtg ctgacggcgc gggttgcagg aggtccatcc ttctgctacc
     7861 cgctgttaga gggccgaagt actatggtca ggatcacgat tagggtaagc gcctggcatc
     7921 gagaaacggg ccgagctgcc cacggtccag cagatcacgc cgacgaacca gccattgtgg
     7981 agagtcggcg aatagggccg ttgccgcctc gaccgtgaga ggtttcgcga agtagtatcc
     8041 ctgggccagg tcggatccca tctgctggag caggaggagt tgctcggcgc tctctactcc
     8101 ctccgcgata gccttcatgt ccaggctgtg ggccaggtct ataaccagtt gcacgataga
     8161 ggtgtcctgg gcgttctccc ccaatcccgc tacgaacgac ttgtcgatct tgaggttatc
     8221 gagggagaac gtcctgagtg cgccaagcga ggaatagccc tggccgaagt cgtcgatagc
     8281 cagctgcaca ccgagtcgct tcagatcctt gagcagcccc gccgcatact ccacattctc
     8341 ggtaaccacg gtctccgtga tctccagctg gaggcgggac acctccagac ccgtgtcccg
     8401 caaaatcttc tcgacagcac ccacgaagtc tggatgctgg aactgcttca cggacacgtt
     8461 gacgctgaga accaggggag agccgtccga gctgagccgc gtccattcgc gtgcctggcg
     8521 acaggcattc tgcataaccc aatgtcccag aggtatgatc aggtccgtct cctcggcgag
     8581 tggtatgaac tcttccggat agatcaagcc gcgttcggga tgctcccagc gcaccagggc
     8641 ttccatgcaa gataccttgc ccgtgctcag gcgcaccatg ggctggtagt agagcgtcaa
     8701 ctcttccttt tctattgcca gtcgaagctc gttctccagt tccagccgct tcctagctcg
     8761 ggaatacatg ctccagtcga acaccctgga gcgccctttg ccttccttct tggcttcgta
     8821 cagcgccaca tccgcgttgc gcaggagggt ggtcgggtcg gcttcattgg ctttgccgag
     8881 agcgatacca atgctcgtgc tgatatagac ctgacgaact cccaactgca cgggagcctg
     8941 caccactttg tggatccggt cggagacttt gatcacgtcg tcgatatccg cgatgccgtc
     9001 cagaacgacg gcgaactcgt ccccgctgag cctggccggg gtatcggcgg gacgcagaca
     9061 ggatagcaag cgccgggcga tcacaaccag caactcgtcg ccagccgtat ggccgagact
     9121 gtcgttaatg accttgaagt tgtcgaggtc tatgaacaac actccatagc tgaggcgttg
     9181 cttcgatgag cgctctacgg cttccgcgag ccggttcgtg aacagcgagc gattgggcag
     9241 gtcggtcaag gaatcgtgga gtgcccggta acgattctcc ctgtaggcgc tgtcgacggt
     9301 attggccacg aagtatacga tcaggcttaa ggtcgcgaag acgaacaacg agagataggt
     9361 gatggaagcg tcgcccaaga ccagggtgcc cagcaccact atggcgccca ccgccagccc
     9421 ggcccagggc acgatcagga aactcgccac gaagacgggc acgcacatga cgatgtaggt
     9481 ttcggccagg tcctgctgcg agaggaagac gaccggacca accgtcatcg tcgtcgtggt
     9541 gaggatggcg gcgatgcgta catacccgag gcggtttagc atgaacaggc ctacgatgag
     9601 ggacaggaat acaccgttgg agatattttg ctcgtgcgat ccctgtatca ggttgaaccc
     9661 ggctagaccg attccgctcc caaggatccc gaggataaag acggcgagga tacgcccctt
     9721 gcgccggact tcggaatctc gggtgtagat cttgagcaag actaccggac catcagatca
     9781 gtaaagcctc gaccttcgat cggcgagcct gtcactcttg caaccgcgca gagactctgg
     9841 cggccagcgc ctgccgcact cgagacgggg gtttgctccg accgtgcctg gacatcgggt
     9901 gccaggcacg tcagttttct tcgcttcggt cccacctaca gtcctttgtc accgaacgtg
     9961 tccagaaaca tgcccagggc ggccaacacg atgctacgga tcacgaaggt aggcgcgtac
    10021 aatgctttcg cgatccacaa ctccgggtag tcatccttga acggcatctc gttcgctatg
    10081 aggtaacggg cggcgaggcg caaacgttct tgcgcgacgc ggcggttggc gcgatagtag
    10141 ctcaggaggg cgagcaagga cagcgcggtt tcctctactg tgggtccgta ctgaccccac
    10201 gagccgtccg tgtgctgggt cgagaggagc caatccaggg tgacctccat ccggcccggc
    10261 gcatgaggga gcaaagccat cacggcctgg ctcgtcggat agtacggcga agcatgccac
    10321 ttgtcctccc aataaccgcc gggctgacgc gcgtcgagca cgtagcctat caccttctcg
    10381 cgagtccgct gccgcgctgc ggctggaagc gtctccaggg cctcgagaat atggaggttc
    10441 gcgcttattg acgggtcgag ctcgcccggg taaacagcga agtggtcatc gcgttcgtac
    10501 tggagcaggc acgacccgtc gacgtcgtag ccggcgttat gcagcacgaa gagagtcacg
    10561 gcggtatcgt ccgcgtcggg gaacacgtgt ggggagaagc cgacgccttc ggcggtcaga
    10621 tgcttgcgaa ggtaccgata atgcgggcgc aacaggctct cgtagccgga gagcaggcgg
    10681 ccatgctgca ggtagtgaag tacccaagcc cgggtgaata tcccgcatgg agcgatggcc
    10741 ggcaaacccc cgtcgtaccg tcccaggagg ttctccaggt acccggtgct gcgcggaaaa
    10801 cgaacccgcc agtctgcggc ttggccgaga agcgcggccg tcgccgaggg tgagctggcc
    10861 atcgatccat cctgctgaag taggcttgtc gcgtcgttca ggtcgatgtc gtccacgaag
    10921 gcttccagcg agaacagcgc ggtggtggtg ctctgaaaga ttcgctcgct cggtagcagg
    10981 ttcagcttct ttacgcgtgt ggtctcgtag cgcctcaggt gggagtatgg gaggttcgca
    11041 cccatcctct tgcccaactc gaggagggct gggagaagaa gctcgaaccc cacggtcgcc
    11101 tgcccatcgt ggtccagctc actcgctact ctccagatgt agtgttcgcc caccgcctgc
    11161 cgctccttgt cgcgcctccg gtttccaagg cgggccagca acaacacaac ggccagcgtg
    11221 gtcaacagac ggtcatggct gtgggatact cggccgcccc acccgccgtc ggagtgctgg
    11281 cgctctagca gagtcggcag cacgtcaggg taggacgggc tcccgtcttc ctcgacaaga
    11341 taggcggccc agctggaatc gtaatcggta ggccgcgtca gcaggctcag gtccgttcgc
    11401 agtagctcgc tcacggcaaa gggcaggtaa cgagacaggc gcgctgcgtg ggcgtatcct
    11461 ttaatgacca tgctcgactt cgaccatcgt gacggctcac ccctcacatg gggacgaggc
    11521 ataagtgacg gtagacgcca ttttgccccg tcagccatgc atctaccgcg attactatag
    11581 tgtttccttc gctagttctc aacaatatcg gctaggaggt aagcagcgaa tccggacgta
    11641 caacacgcgc cacctccgtt ggccgagtgg ctgcggtggt cccgcgagcg ggtggcgcgc
    11701 tggatcttgt ccagaccgcg ccctgttgtg atgggctggc ccttcaacgg aacccgtcgc
    11761 tggtacctcc tccagagagg caggcacccg aataccgacg attacgtggt aaccctgatc
    11821 cgacgccagg cagaactcca ccgtttgatc tttgcacatg ggatcagcgt gataatggca
    11881 ccgggtttcg gtagcgaact cctcgctcgg ggaacggact acacgcgcct tgtcctcggt
    11941 ggcctgttac ggctcgtcga agacccagtc taccaggaga tgttctcttc cgggacgcgc
    12001 atacggttct acggcgactt ccaagaagtg ctcgatacgc cggccttccg gccgatgttg
    12061 cgggcctgcg cggacctgac gaccgctacg gcgtcagggg atgggccgct gcttctcatc
    12121 gggctttttg ccgacgcccc ctatccgacc ctggccaggt tgagcgtcga attcgcgaca
    12181 cgtcgaggtc gcccccccga ccgccgagaa ctcatcgaag cctactacgg gctcgacgtt
    12241 cccgacctga gcatctacct gggatttgcc cagccgagtg tgttcgacgt gccgctggtc
    12301 gcgacgggtt tggaggactt gtacgcgact ctcacccctt cccctgacct gaccgaagcc
    12361 cagttgcgaa ggattctcta cgatcacctc gtaacgcgcc gcacgccgac ccctgattac
    12421 gggacgttgt cagccgacgc acgagcagcg ctcaccgagt acaacgagcg tcactctggt
    12481 atcacgcttg gggtggggcg catagatccg cttacgaaag tatggactcc gttgctcccc
    12541 gacaatacct ctgacgggta agctcaacgg caccgcatgg gtggggcatc ccggaggtgt
    12601 tcccggtcga ctccgcgaca tcctcaacgt ttagaagctc gttcatgcgg ccgcgccacg
    12661 ctcctgaacc gatataatcc tggtccgtgg gtattaggga cttcattcag tatctcaagg
    12721 agcatcagga gcgcctcaag agcggtcccg agctctcgga tgtggagcgc gaggagaccc
    12781 tgagagaggc ggagacgatg gtcgggatgg cagacgcggc tatcagggag ctcgagtcgg
    12841 tcctcggaaa ccggcgggtc aaccttcccg aagagcacgc cgtgcgcttc gcccaggacg
    12901 tcttcctcta cctcagagag gaagaagaga aggtcaaggc tgccctcagt gcgcccgcct
    12961 ccagggacgg ctgatctccg aagggcctta acgtagcctc tttacgagca ccgcacgggt
    13021 gggggtgatc tcgtaatcgt ccagaaatcg ttccagcacg cgtcctagac ccttcagccg
    13081 gcgctgcttt atctcgggat gttcgatagt gatactgacg acttcctcct cgtccacaga
    13141 ctcgatttcc agaacgcctt ctcgcgcgga gagcaaaacg tccgtaacta cggcttcggc
    13201 agcgacggcc aactcgttca gccttccgcc gccgagcccc gcgacggcac ccctccaggt
    13261 gatgaagtag cgggcctcgc ccgcgcgggc gaggtcgacg ggcacctctc tcttgttggg
    13321 cgaggcatcc atccctttag ggtacaacgt attacaaccg ctcctaacgt ccctcgtata
    13381 gactctatag cgaacgacac actcctggca cctataggag gttctggcac tttgaaatcg
    13441 agatctacag ggatctccgc ctcgctcgcc ttcatgctcg gtacgatcct gctcatgggg
    13501 tgttccgaga aagaaccggg cgtgcaagcg gacaatacgg ccaaggcctc gccgcaggct
    13561 tccatcctgg ccgacgagcc ggtggcaagg gtcgcctcga aagtcgggcc aagcgtcgtg
    13621 caggtcaacg tcagggcggt gcgagagacg ccgctcggaa cccagagggg tgaggggttg
    13681 ggtagcggtg tgatctaccg ccccgacggt tacatcgtca cgaacaacca cgtcatcgaa
    13741 ggggctaccg gcgtgaacgt ggccttcgcc gacggcacca tcgaagtcgc aagcatagtc
    13801 ggcaccgatc cgaacaccga gatcgccgtg ataaaggtag accgcaacgg cctgccggcg
    13861 gccacgttcg acgatgcaag cacacccgtc gtcggccagc tcgcggtagc catcggcagc
    13921 ccgtctggct tcgagtcgac cgtcacctcc ggcgtggtga gcggcgtggg ccgcgagttc
    13981 cctcccgaac taaccatcgg cggtacggcg gagcggagcg ccttgaccga cctcattcag
    14041 accgacgcgg cgatctcacc gggcaactcc ggcggcgccc tggccgaccg cgacggcgag
    14101 atcatcggta tcaacgtcgc ctatctcccc cctgccgaga ccggggcggt agacctcggc
    14161 ttcgccgtcc cttccgacac ggcggtctcg gttgcggacc agctgatcga ggacggcgag
    14221 gtaacgacac cttacctcgg cgtgttgacg acggatctca gccccgagga cgccggacgg
    14281 ttcgacctgc ctatagactc gggcgcgctg gtagagcggg tggtgccggg tagcgcagcc
    14341 tggagggccg gggtgcgcag gggggacata atcacggccc tgggcgacgc ccgtatagtg
    14401 ggctacgggg acctcctcgg cgccctgcgc gaccatgagc cgggcgagag ggtcaccctg
    14461 acggtcttcc gcaacggaga agagaggaag ctcgcagtga cactcggcca gatgtcgcag
    14521 caaggagggt agtcatcatg cagggtgttt gggtccgcag gctgatggag accttcgcga
    14581 tcctgaccct cggggacggg atcatcgcgg tgatcttccc cacggagcac tcactcctgt
    14641 gggagttcgg ccccgcaagg gtccgcaaaa tcgcccgctt cttcgccgag aacccgaacc
    14701 tgatgcgact cctcggcgcg gcccaggttg ccttcggcat ccggctggcg aagcgccaat
    14761 accacggacg atagtcatct tccgcgaacc agccgtggca gtcgcgcggc gatagaatag
    14821 tcccatgggc catcacgaac acgaccaccc ggcgaacgcg cgcggcggta accgccgcgc
    14881 gctggcggtg gtgctcgtcc tgaccgcgag ctacacggcg gtggagatca tcggcggcat
    14941 cctcacgggc agcctggccc tgctcgccga tgcgggacac atgctctcgg ataatttctc
    15001 gctaggggtc gcccttttcg cagcctggct ggcagggaga ccggccacgc cggagaggag
    15061 tttcggctac cgccgggcgg agatcctggc agccctcgcg aacgggatga cgctggtggc
    15121 gatctccatc tgggtcttcg tcgaagccta ctcacgcttc agggagccgg ccgaggtcct
    15181 cggcgcgccg atgctggcca tcgccgcgct cggactcctc gtcaatgcgg tcggcgccgc
    15241 catcctctac agaacagggg gcgagaacct gaacgtggag ggagccctgc gccacgtgct
    15301 cgccgacgcc ctcggctcgg tcggcgcgat ggtcgccgcc gccactatca tccttaccgg
    15361 ctggcgctac gccgaccccc tggtcagcgt cgccatcggg ctcctgatcc tgggcagctc
    15421 ctggacgctc cttagagact cgaccaacat cctgctcgaa gcgaccccgc gcggcctgaa
    15481 cgccggggaa gtaggccggc ggatggcagc ggccgaaggt atagtcgaag tccacgacct
    15541 ccacatctgg accatcacct cgggcttccc ggccctctca gcccacgtgc tcgtcggaag
    15601 gcacgaagac tgccactccc gcaggcgcga actcgaagaa ctcctcaccc acgagtacgg
    15661 catctcccat accaccctcc aggtcgacca cgtgggcgac catcacacct cactccagac
    15721 cctccgcttc ccctcgcgtc ccgactgatg ttggctcttg ctcctaccaa cgtatcaacg
    15781 attgttagaa tgaaaatatg agtgagggct gtgatcgccg gcgagggttg cgaagacgac
    15841 tgccaccggt aagtaagtcg tctgtgcact ctaacaagag acggtgagca agatagggcg
    15901 gctcaagcgg tgggcgcaga ggctcagggt cgaaatctac gcgctatacc tggtctacaa
    15961 ggaccccagg gtgccctggt acgcgcgggc tttcgccgcg gtcgtggtcg cgtacgcctt
    16021 cagtcccata gacctgatac cagatcctat ccccgttctc gggtacctcg acgatctggt
    16081 catcattccg ctgggggtcg cgctcgcgat aaagatggtt ccaccccgag tccttgccga
    16141 gtgccgggaa gaagcgcgtg gggtagagga caggcctgtc ggcaaagtgg ctgccgtggt
    16201 agtggtggcc gtgtggatct cgctcgcggc gttggccgta tggctcgtgg cgcgggtgtt
    16261 atgaagcctc tcggagaagc gggctgcatg tgctacgatc tcaccgcgga ctagaaagag
    16321 gaagatcttg gcaaagagca gcagaagaag ccggagcagc gagtcaggag gttccaggag
    16381 ggcatacctg attgtaggtg cggtcatcgt cgcattcgta gcaggattca tcgcgctcgc
    16441 ggtgctcgat gccaggcagc ggtccggcgg cgccactggc gacgtaaaga cctacgacgt
    16501 aggccaggcc ggagagcaca cccaaggaga agtggattac gcgcagaatc cacccgcggg
    16561 gggcgagcac aatcctgtgt ggcagaactg cggcttctac ggcaagcctg tccgcgacga
    16621 gagtgccgta cactccctgg agcacggggc ggtctggatc acctactcac cagatcttcc
    16681 gcaggaccaa atagatgaac tgcggggcat cgcagagagc cagtccttca tcctggtcag
    16741 ccccttccct gacctgcctt ccaacacccc cgtggtggcc tcggcctggg gcaagcaggt
    16801 gggcctgagc gggtcggacg accccgatct ggagaacttc atccaggcct accagcaggg
    16861 tccccagacc cctgagcctg gcgccgtctg caccggcggc acgagcgcga cgctttaggg
    16921 ctgtgcggaa ggattgtcct tccgcacagc caggcaacag gtttcaggct tcaggtgtct
    16981 gctctacaaa caaagtcgag gattcgacta atctgagaag ggctttgaga tagagttcga
    17041 gtaattctac gagctcgcgc gatcggccca ctgcttcacc tgaaacctgc agtggttctc
    17101 gaattgaata agtctggagg cgccggcccg ttttctccca tacatcgaat ccctttgacc
    17161 cctacctgca gcctgatgcc tgcggtttgc aaacagaagg cgcagccttt ttgcaaaccg
    17221 ctttgacttc agtctcgccg gacgacgccc tctctccagg cgtagacggc ggcctgggta
    17281 cggtctgcca ggtgtagttt ggagaggatg ttcgagacgt ggctcttgac cgtcttctcg
    17341 ctaacgtaga ggcggcccgc gatctccgag ttgtgcagtc cttcggcgat caacttcagc
    17401 acctcgagct ccctttcgga gaggtcgtgg aagacgtttg gttctgcgcg cctggcaccg
    17461 tgaagctccc tgaccacccg cgccgccacc cgcgggtgca gcacggcctc gcccgaggcg
    17521 gcttttcgga cggcctcggc gagttcgtcg ggcccgatct ccttgaggac gtaggagagg
    17581 gcgccggccc ttatggcggg gaagatgtgc tcgtcgtcgt ggtaggaggt gagcatgacg
    17641 acgttcgtgc gcggcgagct agcgacgagg cgccgggtcg tctcgacacc gtccatcccc
    17701 ggcatgatga ggtccatcag ggcgacgtcg ggagcgtgct cggcggcgag cctcacggcc
    17761 tcctcaccgc tttcggcctc ggcgaccacg ctgatatcgt cttgcgtctg caagaaagcg
    17821 cgtaccccct ggcgcaccag cgcgtgatcg tccaccagaa ggaccgtgat cggctcgctc
    17881 atatatcctc gcctctcttc tcctg
//
LOCUS       DATMVF010000213         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_70783, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000213 DATMVF010000000
VERSION     DATMVF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70783"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VN178_10800"
     CDS             <1..397
                     /locus_tag="VN178_10800"
                     /inference="COORDINATES: protein motif:HMM:NF040059.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81502.1"
                     /translation="DGSRRRPIKGTADHEPAIPDAATLVVAVGNVRALGTPVDEEHVH
                     RPEIFSDLTGIGQGQSITPRAFAVALSKGSLASVPQRAGTAVLITGVEPGRSMADASV
                     ITRELWRLGVRNVILASLPTESAVRVWIP"
     gene            385..1368
                     /gene="gluQRS"
                     /locus_tag="VN178_10805"
     CDS             385..1368
                     /gene="gluQRS"
                     /locus_tag="VN178_10805"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWS81503.1"
                     /translation="MDPLDTSDRSLPSRQQRGCGEKNVSHGRFAPSPTGPLHLGNLRT
                     ALLAWLFARSAGARFLMRVEDLDHSRVRPGVEEAQLYDLGALGLDWDGPVVRQSERMG
                     LYEEAIARLDAGGLLYPCYCTRAEIRAAASAPHGIPASDRYPGTCRELTAAQRAVREA
                     SGRPPALRVRSDDARVAFEDRLLGRCEEEVDDFVVRRNDGTPAYQLAVVVDDAAQGIG
                     EVVRGADLADSTPRQILLARLLGLPEPRYAHVPLVLGPDGRRLAKRHGAVTLSDRAAL
                     GEGPDEARAWMARSLGLAGAGETPSPDDLIARFDPQRLPREPTLWSLQSAV"
     gene            1480..2667
                     /locus_tag="VN178_10810"
     CDS             1480..2667
                     /locus_tag="VN178_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWS81504.1"
                     /translation="MDVSQKFEGLQAEIAENFGEKIVALRRDIHREPELGFGTERTAE
                     KVLAALDGLPVDVETGVAENGIVATLEGEGDGRTVALRADMDALPIQEETGLPFASEI
                     EGRMHACGHDGHTSMLVGAAHALSGMRDRLGGTVKFVFQPAEEGGGGGKVMVDEGVVE
                     DVASIFALHLWPGLPFGNVATKAGPIMAAADAFEMEIVGSGGHGAMPHLAADAVVIAA
                     QVVTALQTVVSREVDPVESAVLTVGEIGAGTAFNIIPEKARLGGTVRTLNSDLRERMP
                     ERMEAVARGVAKGMRGDAKLDYTFSYPVTVNDESAAGYALGVAEDLFGAHSVQELPKP
                     SMTAEDFSYYLEEVPGAFTWLGVGEDVSGLHTPQFAFDEEVLARGSALLTALALEASQ
                     HFS"
     gene            complement(2745..>3397)
                     /locus_tag="VN178_10815"
     CDS             complement(2745..>3397)
                     /locus_tag="VN178_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HWS81505.1"
                     /translation="RGQRPARARGRSQRAARVVVFRGGDVGYGGESPEDFWGALRGVW
                     FDPIHFFRRLDPRGGAIRPAVFVSVILYLDLLLEATLQMLWLREFNYAMIYAPFLGLV
                     VAIVLAPLLVAGLAIIILFILGGGKLSRDGFGPLFRSLGYSSAIGFVLWIPFAPLLAI
                     PYGAFVATVAVKETMNVSPARAAAATLIPLGAVLLTVLALLGPGEAAGFLINPPGS"
BASE COUNT          610 a         1117 c         1134 g          536 t
ORIGIN      
        1 cgacgggtcc aggcggcgcc cgatcaaagg caccgccgat cacgaaccgg ccatcccgga
       61 cgccgccacg ctggtcgtcg ccgtcggcaa cgtccgcgcc cttggcaccc cggtggacga
      121 agaacacgtc catcgtcccg agatcttctc cgacctcacc ggcatcggcc agggccagag
      181 cataaccccg agagcattcg ccgtcgccct ctcgaaaggc tccctggcca gcgtcccgca
      241 aagggccggg accgccgtcc tgattaccgg ggtcgaaccg gggcgaagta tggccgacgc
      301 ttccgtcatc acgcgcgagc tctggcgtct cggcgtcagg aacgtcatcc ttgcctctct
      361 ccccaccgaa tcagcggtcc gagtctggat cccttagaca cctcagaccg gtcgctacca
      421 tcccgacagc agcgagggtg cggcgagaag aacgtctccc acggacgctt cgcgccgagc
      481 ccaacaggcc cgctccatct cggcaacctg cggaccgccc tcctggcgtg gctgttcgcg
      541 cgttcggccg gggcgcgatt tctcatgcgc gtggaggacc tcgaccactc gcgggttcgt
      601 cctggggtgg aggaggcaca gctctacgac ctcggggccc tcgggctcga ctgggacggt
      661 ccagtggtgc gccagtcgga gcggatgggg ctgtacgagg aggccatcgc tcgactcgac
      721 gctggcggac ttctctaccc ttgctactgt acgcgggcgg agatccgggc cgccgcatcc
      781 gcgccgcacg gcatcccggc ttcggaccgt taccctggca cctgccgcga actaacagcg
      841 gcgcagcgtg ccgttcgcga ggcttcagga cgccctccgg cgctgcgggt acgttcggat
      901 gacgccagag tagccttcga ggacaggctc ctcggccgct gtgaggagga ggtggacgac
      961 ttcgtcgtgc gccgaaacga cggcactccg gcctaccagc tcgccgttgt ggtcgatgat
     1021 gccgcgcagg ggataggcga ggtggttagg ggtgctgatc ttgcggactc gaccccgcgc
     1081 cagatcctgc tggcacgact gctcggcctg cctgagccca ggtacgccca cgtccccctc
     1141 gtgctcggcc cggacggacg gcgtctcgcc aaacgccacg gcgccgtcac cctctccgac
     1201 cgcgccgccc ttggcgaagg acccgacgag gcgcgcgcct ggatggcccg ctcactcggt
     1261 ctcgccgggg ccggagagac accgtcaccc gacgatctca tcgcccgctt cgacccgcaa
     1321 cgcctgccgc gcgagccgac cctctggtcg ctacagtccg cagtctgagc cgcgctctat
     1381 ggtacctttc ccttaaggct ccacagcggt gtcagcgctt cagcaaccaa agagctgacc
     1441 gctgaaagct gactgctgac cgccaagata ggagatttca tggacgtatc ccagaagttc
     1501 gaagggttgc aggcagagat cgcagagaac ttcggtgaga agatcgtcgc cctccgccga
     1561 gatatccacc gcgaacccga gctgggcttc ggtacggaga ggaccgccga aaaggtgctc
     1621 gcggcgctcg acggtctgcc cgtcgacgta gagacgggag tcgccgagaa cggtatcgtc
     1681 gccacactcg agggggaagg ggacggacga accgtggccc tacgggcgga catggacgcc
     1741 ctccccatac aggaggagac gggcctgcct ttcgcctcgg agatagaggg caggatgcac
     1801 gcctgtggcc acgacgggca caccagcatg ctcgtcggag cggcccacgc gctttcgggg
     1861 atgcgggacc ggctaggcgg caccgtcaag ttcgtcttcc agccggcgga ggaaggcggg
     1921 ggtggaggga aagttatggt ggatgagggc gtcgtcgaag acgtcgcttc catcttcgcg
     1981 ctgcacctgt ggccggggtt gcctttcggg aatgtggcga cgaaggccgg tccgatcatg
     2041 gccgctgccg acgccttcga gatggagatc gtaggatcgg gcgggcacgg cgcgatgccc
     2101 cacctggccg ccgacgccgt agtcatcgcg gcccaggtgg tgacggcgtt gcagaccgtc
     2161 gtctctaggg aggtcgatcc ggtcgagtcg gccgtcctta ccgtggggga gatcggcgcc
     2221 ggaacggcct tcaacatcat ccctgagaag gcccgcctcg ggggaacggt ccgtaccctg
     2281 aactcagatc tccgcgagag gatgccggag cgtatggagg ccgtagcccg cggggtcgcg
     2341 aaggggatgc gcggcgacgc gaaactggac tacactttct cttaccccgt aaccgtcaat
     2401 gacgaaagtg cggctggcta cgccctcggc gtcgctgagg acctcttcgg cgcgcactca
     2461 gtacaggagc tacccaaacc ttctatgacc gcagaagact tctcgtacta cctggaagag
     2521 gtacctggag cctttacctg gctcggcgtc ggggaagatg tttcagggtt gcacacgccc
     2581 cagttcgcct tcgacgagga ggtcctggcg cgagggtcgg cgctgctaac ggcgctcgcg
     2641 ttggaagcta gtcagcactt cagctagtca gcaaggacct acccatacga aggcgacaag
     2701 ctgaaatgct gacaaggcgc gaagcgccgt gctgacatgc taattcagct tcccggcggg
     2761 ttgatgagaa agcccgctgc ttcccccggg ccgagcaggg cgagcaccgt caggagaacg
     2821 gccccgagtg ggatgagggt cgcggctgcg gctcgagccg ggctaacgtt catggtctcc
     2881 tttacggcga cggtggcaac gaaggcgccg tatgggatcg cgagtagggg ggcgaagggg
     2941 atccagagca cgaaacctat tgcgctcgag tagcccaaag agcggaacag gggcccgaag
     3001 ccgtcgcgcg agagcttgcc ccctccgagg atgaatagga tgatgatcgc cagcccggcc
     3061 acgagcaacg gtgcgagcac gatcgcgacg acgagcccga ggaagggggc gtagatcatg
     3121 gcgtaattga actcgcggag ccacagcatc tgcagcgtcg cctcgaggag caggtctagg
     3181 tagaggatca cggagacgaa gacggcgggc cggatcgctc ctccccgcgg gtcgagtctc
     3241 ctgaagaagt ggataggatc gaaccagacc cctcgcagcg cgccccagaa gtcttcgggc
     3301 gattcaccac cgtatcccac gtcgcccccg cggaacacta cgacccttgc tgctctttgg
     3361 cttcgtccgc gggctcgcgc aggacgctgc ccacgcg
//
LOCUS       DATMVF010000214         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_173630, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000214 DATMVF010000000
VERSION     DATMVF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173630"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VN178_10820"
     CDS             complement(<1..296)
                     /locus_tag="VN178_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWS81506.1"
                     /translation="MTEQKYFRKGFKMRENVQHLLDRDYRSEVVDRVRENGNIFEAEG
                     LTIRLADEFGFCYGVDRAVDYAFQTREKFPDRRIFITGDMIHNASMNDRLREMG"
     gene            431..820
                     /locus_tag="VN178_10825"
     CDS             431..820
                     /locus_tag="VN178_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWS81507.1"
                     /translation="MDRRATIMVADDDAEALGRSASALENAGFRVVSATDGRTALQEA
                     LTRRVDLIIMDVSIPQLSGVETCHCLKAMPTTQKIPVVLTAAKKDPAAKALAERTQGS
                     VRVLRKPFTDEDLLSVVRQVARPKSLI"
     gene            1072..2595
                     /locus_tag="VN178_10830"
     CDS             1072..2595
                     /locus_tag="VN178_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HWS81508.1"
                     /translation="MGLSKRRKALITLATLIGTFLAAVDATVVGTAMPTVIGELSGLE
                     LYPWAFASYLLAATVTGPVFGKLSDTYGRKPVYLAGIFLFLLGSVLAGTSQSMAALIF
                     FRTIQGLGAGAVQPVAITIVGDIFELEARARVQGLFGAVWGISAVVGPAAGGFITDLI
                     SWRWVFYVNVPFGIIAAVLLAATLTESFERRERSMDYLGTALLTGGLVAVLLALLGSG
                     GAGELSVATFGLFFGGLAVLALFVLVERRAGDPVVPLALFSERIIAVSALGNVAMGGV
                     LLGVSVYVPLFVQGALGGTAITAGAAVASLSIGWPVGSFVGGRLLLLTGYRSTLLLGS
                     ALIAVGSALCVPMDSGTSLSYVVFAVVVIGLGLGFSSTSYLVSVQNAVPWRRRGVATS
                     SVVFFRTVGGTIGVAVMGALLNASLGERYRAAVDRATGGDAGLGRLLSDPNALLQPAV
                     RSRIPEAAYSEVAGALAASLSPAFWVLLFFGLLALAVATLFPHGTAKDLVSREEDYG"
     gene            2588..3241
                     /locus_tag="VN178_10835"
     CDS             2588..3241
                     /locus_tag="VN178_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81509.1"
                     /translation="MDETGSELTLEDLPSPPLFKLVDPEGRDVFQETEVGGEGARVCA
                     LFSDRELAGEFSAGAAQHGMERLSGLNPRALSDWGSIEVFALSGADFVLVVSERGAGL
                     FHAGDVAQKAEEMAGEIPFPLYMISDERGEAPLITVEVEDGEVLVAALFSSREHASDF
                     RERAAHLDLPDSLGTIDDRDGLRRHALVAREAGATYAVIDPASGLTEAIPVEELIEP"
     gene            3238..3825
                     /locus_tag="VN178_10840"
     CDS             3238..3825
                     /locus_tag="VN178_10840"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81510.1"
                     /translation="MKPTLCTDVDSTIWNTGAWVCSAVLDITGETLDIDRITTWTHVL
                     DTYGEKMTTAIFDRVFDPERVRDREPYPGAPEVLRALQERHGIMIHFVTHNDQEIMTP
                     HLESWLRTYFGPKVDLTVTTEDKLGILDALGAFGLIDDRPDTIERVADAGLWAAAMQQ
                     PWNRDLLTRRNDVHGFSHWQEVPDLLPLEYRMGRS"
     gene            3880..4113
                     /locus_tag="VN178_10845"
     CDS             3880..4113
                     /locus_tag="VN178_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81511.1"
                     /translation="MESAESNEGIRADAPRIECFLGRGSQVTVAARTGVDLAGYVCDR
                     NESGLLLDARDPSGDPTGYEFLPWSSIERLSKG"
BASE COUNT          745 a         1369 c         1332 g          782 t
ORIGIN      
        1 atacccatct cgcgcaggcg gtcgttcatg gaggcgttgt ggatcatatc ccccgtgatg
       61 aagatccgcc tgtcggggaa cttctccctg gtctggaagg cgtagtccac ggccctgtcc
      121 accccgtagc agaatccgaa ctcatcggcg aggcggatcg tgaggccttc ggcctcgaag
      181 atgttgccgt tctcacggac cctgtcgacg acctcagagc gataatcccg gtccagaaga
      241 tgctggacgt tctcccgcat cttgaagcct ttacggaagt atttctgctc cgtcacgctc
      301 acctctcgta cccgaagttc gtttcccgtg ctcgaagatg agtgtagcgc atctaccccg
      361 acgcgtcgga gaagagtgtg gcgcgcatca cgctcgctgc aaatgtgaat tgggtgtaaa
      421 atctagcgcc gtggaccgca gagcaaccat catggtcgca gacgacgacg cagaagccct
      481 gggacgctcc gccagcgccc tggagaacgc gggcttccgc gtggtctcgg caacggacgg
      541 ccgcacggcc ctccaggagg ccctaacccg cagggtggac ctcataatca tggacgtttc
      601 gatcccccag ctgagcggcg tagagacctg ccactgcctc aaggcgatgc ccacgaccca
      661 gaagataccc gtagtcctca ccgctgccaa gaaggacccg gccgccaaag cgctcgccga
      721 acgaacgcaa ggctcggtgc gggtactgag aaagcccttt acagacgaag acctcctctc
      781 cgtcgtccgt caggtcgccc gccccaagtc tcttatatag ctggtcagca cgctcagccc
      841 ttcgggcttc gcttgtcagc aagtcagcat ttcagcttgt tgttttcggt agaaagctct
      901 caccagtaga cgaacgagcg cattgcagtg aacctatgat cctgcctctc gccttacggc
      961 gtcgcgcgta aagctcaagc caaatagctg accagctgca gagcgaagcg acgcgaagcg
     1021 acgcggtggt aagatcgctc cccgtaaagg ccgctacagg agcgctgtct cttgggactc
     1081 agcaagcgcc gcaaggcgct cataacgctc gctacgctca tcgggacctt cctggccgcc
     1141 gtggatgcca cggtcgtagg gacggcgatg ccgaccgtga tcggggagct cagcggcctc
     1201 gagctctacc cgtgggcttt cgcctcttac ctgctcgcgg cgaccgtgac gggccccgtc
     1261 ttcggcaaac tctccgatac ttacggccgc aaacccgtct acctggccgg gatcttcctc
     1321 ttcttgctcg gcagcgtgct cgctgggacc tcgcagagca tggcggccct catcttcttc
     1381 aggacgatcc agggtctcgg agcaggtgcc gtgcaacccg tcgccattac catagtcggg
     1441 gatatcttcg agctcgaggc gcgggcgcgt gtccagggac tcttcggggc ggtgtggggg
     1501 atctctgccg tggtcggccc cgcggccggc ggtttcatca cggatctgat ctcctggcgc
     1561 tgggtcttct acgtcaacgt gcccttcggc atcatcgccg ccgtcctcct cgcagcgacc
     1621 cttaccgagt cattcgagcg tcgcgagcgc agcatggatt acctgggcac ggccctccta
     1681 acaggcggcc tcgtcgccgt gttgctcgcg ttgctcggaa gtggaggcgc cggagagctc
     1741 tcggtggcga ccttcggact cttcttcggc gggttggccg tgctcgcgct cttcgtgctc
     1801 gtcgagcggc gggcgggaga ccccgtggtc ccgctcgccc tgttctcgga gcggatcatc
     1861 gccgtgtcgg cgctggggaa cgtggcgatg ggcggtgtct tgctcggcgt ctcggtctat
     1921 gtgccgctct ttgtacaggg cgccctcggt gggactgcga tcacggccgg cgccgccgtg
     1981 gcttcactct ccatcgggtg gcccgtggga tctttcgtcg gcgggaggct gctcctgctc
     2041 acggggtacc gctcgacgct cctactcggc tcggccttga tcgccgtcgg ctcggccctg
     2101 tgcgtgccca tggatagcgg aacctcgctc tcctacgtcg tgtttgccgt agtggtgatc
     2161 gggctgggac tcggcttctc gagcaccagc tacctcgtca gcgtgcagaa cgccgtgccc
     2221 tggcgccgtc gcggcgtggc cacctcctcg gtagtctttt tccgtaccgt cggaggtact
     2281 atcggggtcg ccgtgatggg cgctctcttg aacgcctctc tcggcgagcg ttacagggcc
     2341 gccgtggaca gagcaacggg gggcgacgcc ggcctcggtc gcttgctctc cgatcccaac
     2401 gccctgctgc agcccgccgt gcgcagccgc atccccgagg cggcgtactc ggaggtggcc
     2461 ggcgccctcg ccgcctcgct cagcccggcg ttctgggtcc tgctcttctt cggtctcttg
     2521 gccctggcgg tggccacgct cttcccacac ggaacggcca aagacctcgt aagcagggag
     2581 gaggattatg gatgagaccg ggagcgagct gaccctcgag gacctcccca gcccgcccct
     2641 cttcaagctg gtcgatcccg aaggaaggga cgtcttccag gagaccgagg tcggcggcga
     2701 aggagccagg gtctgcgccc tgttctcgga cagagagctc gcaggtgagt tctccgcagg
     2761 agccgcccaa cacgggatgg agagactctc tggcctcaat ccaagggcgc tctccgactg
     2821 gggctcgata gaagtgttcg cgctctccgg ggccgacttc gtgctggtcg tctccgaaag
     2881 gggcgctgga ctgttccacg ccggtgacgt ggcccagaag gcggaggaga tggcaggcga
     2941 gatccccttc ccgctgtaca tgatctccga cgagagggga gaggcgcccc tgatcacggt
     3001 cgaagtcgaa gatggagagg tactggttgc agcgctcttc agctcgcgcg agcacgccag
     3061 cgacttccgc gaacgggcgg cccacctcga cctgccggac agcctcggaa ccatagatga
     3121 tagggacggc ctcagacgcc acgcactggt agcgcgcgaa gccggagcga cctacgctgt
     3181 catagatccc gcatccggcc tcaccgaagc gatccccgtc gaggagttga tagaaccttg
     3241 aaaccgaccc tctgcacaga tgtcgactcg accatctgga acacgggcgc ctgggtctgt
     3301 tcggcagtcc tcgacatcac gggcgagacg ctagacatcg acaggatcac cacctggacc
     3361 cacgtcctcg acacctacgg cgaaaagatg acgaccgcca tcttcgaccg cgtcttcgac
     3421 cctgagcgcg tccgggaccg ggaaccctat cctggagccc cagaggtgct gcgcgcactc
     3481 caagaaagac acggcataat gatccacttc gtaacccaca acgaccagga gatcatgacc
     3541 ccccatctcg aatcctggct cagaacctat ttcggcccca aggtggacct taccgtcacg
     3601 acggaggaca agctcggcat cctggacgca cttggcgcct tcggcctcat agacgacagg
     3661 cccgacacca tagagcgggt cgccgacgcc ggtctgtggg cggcggccat gcaacagccg
     3721 tggaaccgcg acctcctcac cagaaggaac gatgtacacg gcttctccca ctggcaagaa
     3781 gtccccgatc tcctgccgtt agagtacagg atgggtcgct cctgaaacct gatgcctgta
     3841 acctttcctg tgtcaggtaa gggacaagga ggagacacgt tggagagtgc tgagagcaat
     3901 gagggaatac gggcagatgc gccgcggatc gagtgtttcc tgggccgtgg gagccaggtt
     3961 acggtcgcgg ccaggaccgg agtagacctc gctggctacg tctgcgaccg gaacgagagc
     4021 ggcctgctcc tcgatgccag ggatcccagc ggggatccga ccggctacga gttccttccc
     4081 tggtcgagca tcgaaaggtt gagtaaaggg taggacgagg acccgtcgga acggcgagct
     4141 gatcgggcag gggtcagaga agaaagggag caaaatgatc gggagggtgg gcttcgtggg
     4201 gctcgggctc atgggtgcgc cgatggcc
//
LOCUS       DATMVF010000215        15608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_404257, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000215 DATMVF010000000
VERSION     DATMVF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15608
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404257"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VN178_10850"
     CDS             complement(<1..316)
                     /locus_tag="VN178_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS81512.1"
                     /translation="MVPENWKVRMEMLRRLARANVRGEGPPSVREVGEAVGLRSSQTA
                     YKHLKKLEEGGYIEREGGRARGIRLTEKGWEAAGEMPLLGRIAAGRGLEAVTVGDEAY
                     SLA"
     gene            573..998
                     /locus_tag="VN178_10855"
     CDS             573..998
                     /locus_tag="VN178_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81513.1"
                     /translation="MEASVSEIHRYPLRPEGFEEPLFPTRESRLAVLDHLLRTSWLSR
                     ASEMEERGGQAAHARYEPVELENRHGRVRVTWASGRRRCRKRRHVVEVLGRWREVRAW
                     WDETRAKDRSLARLLLSDGSVIDLARENDGEWFLVGITD"
     gene            995..4273
                     /locus_tag="VN178_10860"
     CDS             995..4273
                     /locus_tag="VN178_10860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWS81514.1"
                     /translation="MNGDSPDDFVHLHVRSGFSFGFGVARPEELVEAAARMGMPSLAL
                     TDRDGLYGIPRLLEAAGEAGILPIVGAQVSTAGGHVVLLAESIEGYRSLCRLITDYRI
                     SSEDRRRPVCSLGKILDHREGLICLTGAVPFGLLPRLVLAGQRKEATRTLHSLQEAFG
                     GRLYVELTDDETATGRRSMGKVAAFAQDAGVPVVATGEVAYLFPADHRLHEVLVAAAN
                     LTALPGPEYRPTDRLHLRSGKEIRRLFEDYPKALENAAGVARQCAGAVKLSGRVHMPA
                     AILPPGKTAGKVLLELTLAGARRRYGRRAGDDPTAEELKSRLGRELRCLHALGFAPYF
                     LIAHEAKEIAKSKGIPVTGRGSAANSLVSHCLGLSEPEPFSNRLLFERFLHEGRKDPP
                     DVDLDFCSVRRDEVRFEMIRRYQHLGVAEAATVQTMSLRGAVRVAARALGHSPQEIDT
                     LSRQVPTRFHDRDRIYAGLSGWEESLAEPAMYGHPLQDREKYRLLLELSAGLAGRIKE
                     GGTHSGGMVFGTAEYHLSELVPLEPSGTVGLLRCQYDKDDLERVGLPKLDLLGLRMHT
                     ALHKAGELASRRLGRRVDPYDLPPEDKKTYALIRTGCNAGIFQLESPGQMHLSQRLGP
                     RRLADLVAQISLFRPGPVRGDLVTPYVKRRNGREAYEVILPELEEILRPTYGVLIFQE
                     QALAVANAVAGFTLAEGDLLRRAMTKDRGPGAMRELRLEFMRRAREKGVPAAKAEEVF
                     SWMEGFSVYGFSAAHAASFAELAYASAYMRRHYPAEFFAAVLDSQPMGFYSPRLLLNE
                     ARRIGLHALPPDVHLSGEDFSIEEEGSALRVGLRYCKGLSERAISSIVAGRRKKPFVS
                     VADLYGRTAVEKDALENLIRGGFLDSLAGGSGRSRLLGESASLPKKRRRGRQEEIPIE
                     HPASWWAMRERRSVGHLPLTDTAKERMEWEVLGLNVHRHPLEPYRDAFRELGVVPSEE
                     VRKLPHGTSARAAGLIECLQSPPTRSGYRVYFLLIEDEWGLLQTTIFRSVYERCGDIL
                     HHEGAFLVEGRVEQTIEKGFAFLIHHIESLRDVLSEARVPVPRVVASPGAFLRAGRRS
                     RKAG"
     gene            4420..4947
                     /locus_tag="VN178_10865"
     CDS             4420..4947
                     /locus_tag="VN178_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS81515.1"
                     /translation="MEYRTRETEVREYLFEDDGRIPNNSTLPMLVYPQALDSSEQDSS
                     RCKELLAENGWGGAWVNGVFSYHHYHSNAHEVLCVVSGSASIAFGGPEGETVEVEAGD
                     AVVIPAGVGHCNKGSDGGFSVVGAYPRGQESYDLRTGEEGERPEVLENVRNVSLPESD
                     PLFGEKGPLTQRWSR"
     gene            complement(4970..5383)
                     /locus_tag="VN178_10870"
     CDS             complement(4970..5383)
                     /locus_tag="VN178_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWS81516.1"
                     /translation="MRYLLDTKVLSEVRRPRGDAGVKRWISSVPTEDLYLSVLTLGEV
                     RRGIGLLSRRDPVQADLYETWLVTVLHDYADRVLPVDATAAEEWGRMNVPDPVSIVDG
                     LMAATAKVRNLTFVTRNTADVARTGVRLLDPFDAS"
     gene            complement(5388..5636)
                     /locus_tag="VN178_10875"
     CDS             complement(5388..5636)
                     /locus_tag="VN178_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWS81517.1"
                     /translation="MSWQLQEAKQKFSELVRRTLEEGPQVVTRHGEEVVVVVPVEEFR
                     RMSKDGEKMDFKEFLMSAPDLSVLDLERQKEFPRDIEL"
     gene            complement(5738..6589)
                     /locus_tag="VN178_10880"
     CDS             complement(5738..6589)
                     /locus_tag="VN178_10880"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWS81518.1"
                     /translation="MESILSLDRAEVFFDGIFSEPRLLHPEGIAIDAGGDIWCGGETG
                     EIYWLSADGSRIETVASTGGFILGLAFDSDQNLYACDMRHSAVFRLDTRTGDLVHFAN
                     GGGEMRIPNFPVVDGSRNCLYVSDSYDPEEQGPGVWRFDLESGEGGLWYDRAMTFANG
                     MALSPDKDHLYVAESFAGRVIRIPIDESGGPQEGEPFVEGLERVPDGLAFDTDGNLYI
                     SCYEPSRIYRVSPEREVHLFIDDPIAHTLCHPTNCAFRGEELFTSNLGRWHITRIEAH
                     VEGLVLP"
     gene            complement(6623..7420)
                     /locus_tag="VN178_10885"
     CDS             complement(6623..7420)
                     /locus_tag="VN178_10885"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWS81519.1"
                     /translation="MTNEVRGKVAIVTGGGAGIGLASAEKLAREGAAVVVCSDREDQA
                     RQAAGRLREEGLEVEGMRGDVTTSADMKALVDFTVENYGGVDVLVNSAGVQRYGTVTE
                     TEEEVWDEVLDVNLKGIYLAARHAIPEMRRRGGGAIVNVSSVQAIASQAGVAAYTASK
                     GGINALTRAMAVDHAEENIRVNAVCPGSVDTPMLRWAADLFKGENTVEATVDDWGRMH
                     PLGRVARPEEVAEVICFLASPRASFVTGADYMVDGGLLAALGVRLPE"
     gene            complement(7424..10546)
                     /locus_tag="VN178_10890"
     CDS             complement(7424..10546)
                     /locus_tag="VN178_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HWS81520.1"
                     /translation="MPRKRPSSDDLRTQRAREELIQGPSAVDFDVHDFSVRVGSLPLF
                     RRTGNGDLSQALRLVVLAESAVGDVTFAISDGDTTLDTVRARIDEGRTLVHLFVPEVY
                     EPRTISLRVEATSREPFEARVEILPQRKWSVFLVHHSHLDIGYTDTQSSVLQHHLQYL
                     DSVLDLTSATDDWPEDATFRWNVEANWPLLHWLKNRPESDRTRFFDRVREGRIEICAL
                     PFSMHTEAYSIDELARQLRFADELREKYDVPIRTAMQTDVPGATIGLLSSLVDADVRY
                     LSVAHNYAGRSAPHLVGGQGLTRPFYWRAPNGKRLLVWYTDSPHGSAYMEGNLVGLAE
                     DYRATLHALPGYLAALSKRPYPYRGRGESIFGWTGLDPSSEVTKVPYPHDVLHLRVQG
                     VFSDNAAPSIVPAEIVREWNEKWAYPQLRLATNSEFFTSAEERLAGSMETYSGDWTDW
                     WVDGIGSGARHLGFNRRAQADIRTAQTLHSLADTLAEDGADPRDEIDETYENMALFDE
                     HTWGAANPWMDGLEMRESGALQWETKAGFAREAYERSHALLRSGVHRLSHAFGRSGDS
                     LASVTVFNPSRWERTDPVSVFVPESRTELQRPLVVVDGDARERVPCVVEDQENAQFRV
                     RGSRFTFIARDVPAFGYRRYDIVEGEDEQEDPKSGDEPCIENEYYLVRFDLARGRVTE
                     ILDKTSGLNLVDADAPFGFNQFVYDRYASAPHFNHLSSRIQATDLALLGERSAGGLAV
                     VAQRSSNPVWDRVTVRLVDERADIVESTLTLFRGVRRLDITNRIHKAGTPEKESVYFA
                     FPFDVGDHSLHYEITGGIDSPDAPRVPGSADHMRAIRHWVGLENERVRLAWATMEAPL
                     VQFGNIHLPYLPFPETIDHEIANPATVYSWALNNIWDTNFPSQQQGEIEFCYAVASGE
                     NMETPELGMRTAAALTTPLLGILSAPSAGNDLPERGSFCSVDHPLLDVVAIAPSRRGH
                     DLVVMLQSLSSESEEVCVSFGLLGVARARVGSHLERHLEEARVDGENVRFVVPAGSLV
                     SVAVDLERGR"
     gene            complement(10830..11249)
                     /locus_tag="VN178_10895"
     CDS             complement(10830..11249)
                     /locus_tag="VN178_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS81521.1"
                     /translation="MKITEADHTNWRVKDIERSLGFYRDVLGLEPFGLDAYERGEHPL
                     VSLRVTPGFILHLRPDPTFEPVSTGGYDHLALVVEGTSPDALAESLTEAGVEIESRSV
                     NVIGARGSGEALYVRDPDGYLIELKLYTNESGSPRGE"
     gene            complement(11364..12683)
                     /locus_tag="VN178_10900"
     CDS             complement(11364..12683)
                     /locus_tag="VN178_10900"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81522.1"
                     /translation="MLGGVPTAFRPLRHRDFRLLWTGLAISLVGSGLWLVALAWQVIA
                     LGGGPTELSLVTALFSVGLLAFVLIGGVAADRLPQRLVMLAADLIRAAVLVILGLLSL
                     SGDLRIWHLAVGGVLIGAGEAFFIPSYTALLPHLLPEDELLAANGLEGTLRPLAQQAA
                     GPALGGVAVAALSPGVAILTGGLAYLVSAGCLLVMKVRPRKVPGTNGAPGVAVVFSDL
                     RDGFRYVRRTSWLWATLLFALALVLFIIGPLEVLLPFAIRDNLGGGAEDYGLALAAFG
                     VGGAVGALAISSGRLPRRYLTVMILMWGLGSLPFAVLGVAGALWVMCAALLVVGATYS
                     AAMVIWGTLLQRRVPEGLRGRVSSLDFFVSLALMPVSMAIAGPAGAAFGITAVFVVAG
                     IVPVFLAVAAIAIPRLDRDELAHPLDHPEPVPAKNLSGSTRNEPKGS"
     gene            complement(12758..13117)
                     /locus_tag="VN178_10905"
     CDS             complement(12758..13117)
                     /locus_tag="VN178_10905"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HWS81523.1"
                     /translation="MNDEPLALIEREVLGWDGIFKKRDEHGPGGIGVTGYRYGDPESG
                     GPQIGHIHDDGHADFRFPREVRDELIRTGRAIPHPAFPDSRTTASYRVESVDDVAGAL
                     ELFRMNYERRKRRNGLV"
     gene            complement(13630..13944)
                     /locus_tag="VN178_10910"
     CDS             complement(13630..13944)
                     /locus_tag="VN178_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HWS81524.1"
                     /translation="MTRYAWVLEVRPGYEEEYLRRHQEIWPEMVEALREAGVSNYSIF
                     RHGLTLFGYFETDDIDKTREYLANSETNQRWSDWMDPIMKVEIDPTTDFPYLLPLQWH
                     MD"
     gene            complement(14211..15083)
                     /locus_tag="VN178_10915"
     CDS             complement(14211..15083)
                     /locus_tag="VN178_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81525.1"
                     /translation="MDAFLIMVAAGCVGALVGAIAAFYLGGVREKRKRILASQGEEQR
                     RLQEWRRKEQSRIEENKEREHQREEERNQRLIERRAEALDDIRIQALYFANAFRSWTD
                     RAANLEVPPKAGVERVVGFSEGLAELLQQADEVSTRLVFLRGHYNAHSPILEEESRKI
                     IEPLEEQGMERHSLLSNQLKATAASLQAFLVLLQPQLVDVMDPTSHSDVISRLAGSGL
                     GTSVSPETFSRVKELWDVHGNKETILEEHRREVLQAANVARDWNIQMHLRALDAKIGR
                     TENISSWARQSFRT"
     gene            complement(15132..>15608)
                     /locus_tag="VN178_10920"
     CDS             complement(15132..>15608)
                     /locus_tag="VN178_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81526.1"
                     /translation="SEVDPLVENLEANQGDTEYLVAAIRSRIASPIILNTDEPVIAFS
                     GFAGRDLVFSIERVAGLVHQGPVRFFIIKERDIEHAAQPGTDRRYLELDEQVPPLQQL
                     PRLKAHLKALADIHEKKQVRWITDNCEQVPEGLWQSSTSKSPTSTVLLYDCGTRSR"
BASE COUNT         2841 a         4971 c         4810 g         2986 t
ORIGIN      
        1 ccgcgagcga atacgcctca tcccccaccg tcacggcctc gagcccgcgt ccggcggcga
       61 tcctacccaa caaaggcatc tcgcccgctg cctcccaacc cttctccgtc agcctgatgc
      121 cgcgcgcccg cccaccctct cgctcgatgt acccaccctc ctccagcttt ttgaggtgct
      181 tgtacgcggt ctgggagctc ctcaagccca cggcctcccc cacctcgcgc acgctcggag
      241 gcccctctcc cctcacgttc gcgcgggcca ggcgcctgag catctccatc cttaccttcc
      301 agttctcagg gaccaccttc gtctcctcta aacgcctcgc aatgacactt tatactattt
      361 tgccgtagac tacaaacaat agtttgtggt agcgtagaga agctggcaaa gcaggagcgc
      421 ccccaccccg ggcctcgccg gccggccggc gtccaaccaa taccggcctg cgagggtttg
      481 aggaggcgag gacgcctgct ccccggatta tatccggagg cgcgggtttc tcatcccccg
      541 gctctcgggc gctccccgga gggaggagag agatggaggc gagcgtctcg gagatacacc
      601 gctacccctt acgacccgag gggttcgagg agccgttgtt tccgacccgt gagtcgaggc
      661 tggcggtcct cgaccatctg ctgcggacct cctggctctc gcgggcctcg gagatggaag
      721 agaggggagg acaggccgca cacgcccgat atgagccggt cgagttggag aaccgtcacg
      781 ggcgtgtgcg ggtcacttgg gcctcgggcc ggagacggtg ccgcaagagg cggcacgtcg
      841 tcgaggtact cggacgctgg cgggaggtcc gggcctggtg ggacgagacg cgggcgaagg
      901 acaggagcct ggcgagactc ctcctctccg acggctcggt tatcgacctg gcgcgggaga
      961 acgacggaga gtggttcctc gtagggataa cggattgaac ggggactccc ccgacgactt
     1021 cgtccacctg cacgtcagga gtggattctc cttcggtttc ggggtcgcga gaccggagga
     1081 gcttgtggag gccgcggcga ggatggggat gccctctctg gcgctcacgg accgcgatgg
     1141 actctacggc atcccccgcc tcctcgaggc cgcaggtgag gctggcatcc tccctatcgt
     1201 cggggcccag gtctcgacgg cgggcgggca cgtcgtgttg cttgccgagt ccatagaagg
     1261 gtaccgttcc ctgtgccggc tcattacgga ttacaggatc tcttcagagg accggcgccg
     1321 tcctgtctgt tcgctgggga agatcctgga tcatagagaa ggtttgatct gcctgaccgg
     1381 agccgtcccg ttcgggctct tgccgcgcct ggttcttgcc gggcaaagga aagaagcgac
     1441 cagaacgctg cattctttgc aggaggcatt cgggggccgg ctctacgtag agctgaccga
     1501 cgacgaaacg gcgaccgggc ggcggagcat gggaaaagta gcggcctttg cacaggacgc
     1561 tggcgtaccg gttgtcgcca cgggagaagt cgcttacctc ttccctgctg accacaggct
     1621 gcacgaggta ctcgttgctg ccgcgaacct cacggcgctc cctggtccgg aatacaggcc
     1681 taccgacagg ttgcatctaa ggtccgggaa ggagatacgg cggctcttcg aagactatcc
     1741 caaggccctg gagaacgctg cgggagtcgc gcggcagtgc gcgggggcgg tgaagctgtc
     1801 gggcagagtc cacatgccgg cggcgatcct gcctcctggc aagacggctg ggaaggtgtt
     1861 actggagctg acgctcgcgg gcgccaggag acgctacggg aggagagcgg gggacgatcc
     1921 cacggcggag gagttgaagt cgcgactcgg acgggagctg cgttgtctcc acgctctggg
     1981 gttcgcgccg tacttcctga tcgcgcacga agccaaagag atcgcgaagt cgaagggcat
     2041 ccccgtcacg ggccggggaa gtgccgcgaa cagcctggtc tcgcattgtc tggggctgag
     2101 cgagcccgag ccgttctcga accggctgct cttcgagcgg tttctgcacg aaggacgcaa
     2161 ggacccgccc gacgtggacc tggacttttg ttccgtgagg cgggacgagg tgcgcttcga
     2221 gatgatccga cgctaccagc atctgggcgt cgccgaagct gcgacggtcc agacgatgtc
     2281 gttgcgagga gccgtgaggg tggcggcgcg ggcgctcggg cattctccac aggagatcga
     2341 cactttaagc cgccaggtcc ccacccgctt ccatgaccgc gataggatct acgccggcct
     2401 ctccggctgg gaggagtcgc tcgccgagcc ggccatgtac ggccatcctt tgcaggatag
     2461 ggagaaatat cgtctcctgc tcgagctctc cgcggggctc gccggccgca tcaaggaggg
     2521 aggcacccat agcgggggta tggtgttcgg gacggcggag taccacctct cggagctggt
     2581 gccgctggag ccttcaggga cggtggggct cctgcgctgc cagtacgaca aggacgattt
     2641 ggagcgtgtg gggcttccga agctggacct gctgggtctg aggatgcaca cagctttgca
     2701 caaggccggg gagctggctt cgaggcggct ggggcgcagg gtggacccct acgacctgcc
     2761 gccagaggat aagaagacct acgccctcat aaggaccggg tgcaacgccg ggatattcca
     2821 gctggaatcg cccggacaga tgcacctgag ccagcgtctt gggcccagga ggctcgctga
     2881 tcttgtggcc cagatctctc ttttcaggcc gggtcccgtg aggggagacc tcgtcacccc
     2941 ctacgtgaaa cgccggaacg gcagggaggc gtacgaggtg atcctacccg aactcgaaga
     3001 gatactacgt cccacctacg gcgtcctcat cttccaggaa caggccctgg ccgtggcgaa
     3061 cgccgtggcc ggcttcaccc tggccgaagg cgacctcctg cgacgcgcca tgaccaagga
     3121 ccggggaccc ggcgcgatgc gagagttgcg cctggagttc atgcggcggg cgagggagaa
     3181 aggggtcccc gccgcgaaag cggaggaggt cttctcctgg atggaggggt tctcggtcta
     3241 cggtttctcc gccgcgcacg cagccagctt cgctgagctc gcctacgcga gcgcgtacat
     3301 gaggcgccac tacccggcgg agttcttcgc ggccgtcctc gactcccagc cgatgggttt
     3361 ctacagcccg aggctgctcc tcaacgaggc gcgtaggatc ggtctccacg ccctcccgcc
     3421 cgacgtccac ctctcaggag aagacttctc catagaagaa gagggctccg ctctccgggt
     3481 tggcctcagg tattgcaagg ggctctccga gagggccatc tcctccatcg tggccgggcg
     3541 gcgcaaaaag ccgttcgtct ccgtcgccga cctctacggg aggacggccg tcgaaaaaga
     3601 cgcgctagaa aatctcatca ggggcggatt cctcgactct ctggctggag ggtcggggag
     3661 atcccggctt ctcggcgaga gtgcatctct tccgaagaag cgcaggcgtg ggaggcaaga
     3721 ggagatcccc atagagcacc cggcgagctg gtgggcgatg cgggagagga ggagcgtcgg
     3781 acatctcccc ctcacggata cggcaaaaga gaggatggag tgggaggtgc tcggcctcaa
     3841 cgtccacagg cacccgctcg agccttaccg cgatgccttc cgagagctcg gggtagttcc
     3901 gagcgaggaa gtcagaaagt taccgcacgg caccagcgcc cgcgcggcgg gtttgatcga
     3961 atgtctccag agccccccga ccaggagcgg ataccgcgtg tacttcctgc tgatcgaaga
     4021 cgagtgggga ctcttacaga cgacgatctt ccgctccgtc tacgagaggt gcggcgacat
     4081 cctgcaccac gaaggagcat tcctcgtaga aggcagagta gagcagacca tcgaaaaagg
     4141 gttcgccttc ctcatccacc atatagagag cttgcgagac gtcctgtccg aggccagggt
     4201 acctgtgccg agggtggtgg cttctccggg agctttcttg cgtgccggga gaagaagtag
     4261 aaaggcggga tagagttacc gtaaacaatt cgtcctcccg atttagccgg attcaataac
     4321 catgttaacc tcgagagatc tcaaagtcat ccagccaacc gcttgctgta ctttgtcggt
     4381 actatgaggt aaacgccgag atgcagccag gagggtcaga tggaataccg gacgcgggag
     4441 accgaagtac gagaatatct gttcgaagac gatggccgca tcccgaacaa ctcgacgttg
     4501 ccgatgctcg tatacccgca agcgctcgac agttccgagc aggactcctc gcgctgcaaa
     4561 gaactgctcg cggagaacgg ttggggcggc gcgtgggtga acggggtctt ctcgtatcat
     4621 cactaccatt cgaacgccca cgaggtgctg tgtgtggtta gcggctctgc gagcatcgcg
     4681 ttcggcgggc cggaaggcga gacggtcgag gtcgaggctg gcgacgcggt tgtgataccg
     4741 gctggtgtcg gacactgtaa caaagggtcg gacggtggat tctcggtcgt cggggcctac
     4801 ccgcgcggac aggaatctta cgatctgcgt accggcgaag aaggcgaacg ccccgaggtt
     4861 ctggagaacg tccgcaacgt ttccttaccc gagtcggacc ccctgttcgg tgagaaaggt
     4921 ccgctgactc agcgctggtc gaggtagcgc ggatctatcg gcactcttgc taggaagcgt
     4981 cgaacgggtc gagcagacgc acgccggttc gcgcgacgtc ggccgtgttg cgggtgacga
     5041 aggtcaggtt cctgaccttg gccgttgctg ccataaggcc gtcaactatt gagacgggat
     5101 caggcacgtt catccgtccc cactcctcgg cagccgtagc gtcaacgggc agaaccctgt
     5161 ccgcgtagtc gtggagaacg gtgacgagcc aggtctcgta gaggtcggcc tgcacgggat
     5221 ctcttcgaga gagtagacct ataccacggc ggacctctcc gagcgtgagc acgctcaggt
     5281 aaaggtcttc ggtgggtacc gaagagatcc aacgcttcac tcctgcatcg ccccgcggcc
     5341 tgcgcacctc cgagagtact ttggtatcaa gcaggtatct cacgacgcta cagttcgatg
     5401 tctcgcggaa actctttctg acgttccaga tccaacacgc tcaagtccgg cgcgctcatc
     5461 aggaactcct tgaagtccat cttctcgccg tccttgctca tacgccggaa ctcctcaacc
     5521 gggaccacta cgacgacctc ctcgccgtgc ctggtaacca cctgcggccc ctcttccaac
     5581 gtgcgccgca ccaactcgct gaacttctgc ttggcttcct gcaattgcca ggacaagact
     5641 tatctcctct gaacaggtat ctagtctgtc tagattggat gtgggagagt ggagagatca
     5701 aggcccgtaa ggctcaaccc ccacgcaaga gaacctttca aggcaaaact aggccttcta
     5761 cgtgggcttc gatccgcgtt atgtgccacc ggccgaggtt ggatgtgaag agctcttcgc
     5821 cgcgaaacgc acagttcgtt ggatggcaca gggtgtgcgc tatgggatcg tcgataaaca
     5881 gatgtacctc tcgttccgga ctcaccctgt atatacgcga gggttcgtag caggagatgt
     5941 acaggttgcc gtcggtgtcg aatgccagac cgtcaggcac gcgctcgagc ccttccacga
     6001 aaggttcgcc ttcttgcggg cctccgcttt cgtctatggg gatgcggatc actctgccgg
     6061 cgaacgattc agcgacgtac aggtgatcct tgtcgggaga gagggccatc ccgttggcga
     6121 aggtcatggc cctgtcgtac cagagccctc cttctccgct ctccaggtcg aatcgccaga
     6181 ctcctggtcc ctgctcttcg ggatcgtagc tgtcggagac gtaaaggcag ttcctggagc
     6241 cgtctaccac ggggaagttg ggtatgcgca tctcgccccc gccgttcgcg aagtggacga
     6301 gatctcctgt tctcgtgtcg aggcggaaca ccgcggagtg gcgcatatcg caggcgtaga
     6361 ggttttggtc gctgtcgaag gccaggccga gaatgaaacc gcctgttgag gctacggtct
     6421 cgatgcggga cccatcggcg gagagccagt agatctcccc cgtctctccc ccgcaccaga
     6481 tgtcgccgcc ggcgtcgatc gcgatcccct cggggtggag gaggcgcggc tcactgaata
     6541 tgccgtcgaa gaagacctcg gctctgtcca gagacagtat ggattccaag atttttctcc
     6601 cttgttctat ttggtcgccg gactattctg gcagtcggac gcccagggcc gcgagcagac
     6661 cgccgtcgac catgtaatcc gctcccgtaa caaagctcgc cctggggctt gcgaggaagc
     6721 agatcacctc ggccacctct tccggcctgg cgacccttcc caggggatgc atcctgcccc
     6781 agtcatcgac cgtcgcctcg acggtgttct cccccttgaa gaggtcagcc gcccatctca
     6841 gcatcggcgt atcgacagaa ccgggacaca cggcattgac ccttatgttc tcctcggcgt
     6901 gatctacggc catcgcccgc gtcagggcgt tgatgccgcc tttactcgcc gtgtaggcag
     6961 ccacccctgc ctgcgaggct atggcctgca cgctcgacac gttgacgatg gcacctcccc
     7021 cacgccttct catctcggga atggcatgcc tggccgccag gtatatcccc ttgaggttga
     7081 cgtcgagaac ttcgtcccag acttcctcct cggtctccgt gaccgtcccg tagcgctgaa
     7141 ccccggcgct gttgacgagc acgtcaaccc ctccatagtt ctccacagtg aagtcgacca
     7201 gggctttcat gtccgcagaa gtggtcacgt ctcccctcat cccctcgact tccaggccct
     7261 cttcacgcag ccggccggcc gcctgcctgg cttgatcctc acggtcgctg cagaccacaa
     7321 ccgcggcccc ctccctggct agcttctctg cggaggcaag cccgatgccg gccccgccac
     7381 cggttacgat cgccacctta ccgcgcacct cgttcgtcat acgctacctc ccacgttcca
     7441 ggtctacggc tacggagacg agactccccg caggaacgac aaaccggacg ttctcgccat
     7501 cgaccctcgc ctcttccagg tgcctttcga gatgcgaccc cacccgggcc cgggcgacgc
     7561 cgagcagccc gaacgagacg cagacttcct cgctctcgga ggacagagac tggagcatca
     7621 ccacaaggtc gtgtccccgc ctcgaaggcg cgatggcgac gacatccagc aggggatgat
     7681 ctacggagca gaagctgccc cgctccggaa ggtcgttccc ggcggacggc gcagagagga
     7741 tacccagcag gggcgtcgtc agggccgccg ctgtcctcat acccagctcg ggcgtctcca
     7801 tgttctctcc cgaggcgacc gcgtagcaga actctatctc accctgttgt tgcgagggga
     7861 aattggtgtc ccagatgttg ttgagggccc aggagtatac ggttgcaggg ttggcgatct
     7921 cgtggtcgat ggtctcgggg aaagggaggt agggaaggtg gatgtttccg aactggacga
     7981 gcggtgcctc catcgtggcc cacgccagcc tgaccctctc gttctccagg ccgacccagt
     8041 gccggatcgc ccgcatgtga tccgccgagc cgggaacgcg cggcgcgtcg ggtgaatcaa
     8101 tccccccggt tatctcgtag tggagcgagt ggtcaccgac atcgaaggga aaagcgaagt
     8161 acacgctctc cttctcgggg gtaccggcct tgtggatgcg gttcgtgata tccagtctac
     8221 gtacgccgcg gaacagggtc agcgtagact ccacgatatc ggccctctcg tcgaccaggc
     8281 ggacggttac ccggtcccag accgggttgg acgaccgctg tgcaaccacg gcgagccccc
     8341 cggccgagcg ctcgccgagc agagccaggt ctgtcgcctg gatgcggctg gagaggtggt
     8401 tgaagtgcgg ggcgctcgcg taacgatcgt aaacgaactg gttgaaaccg aacggggcgt
     8461 ccgcgtcgac caggttcagg ccggaggtct tgtccagtat ttcggtgacc ctgcctctgg
     8521 ccaggtcgaa gcgaaccaga tagtactcgt tctctatgca aggctcgtcg cccgacttgg
     8581 gatcttcttg ctcatcttcg ccttcgacaa tgtcgtagcg cctgtagccg aaggccggaa
     8641 catccctggc gatgaatgta aagcggcttc cccgcaccct gaactgggcg ttttcctgat
     8701 cctcgaccac gcagggcacc cgctctctag cgtcgccatc gaccacgacg aggggacgct
     8761 ggagttcggt gcggctctcg ggcacgaaca cgctgaccgg gtcggtgcgc tcccagcggc
     8821 tggggttgaa tacggtaacg gaggccagtg agtcgccgga ccgcccgaag gcgtgcgaga
     8881 ggcggtggac gcccgacctg aggagggcgt gggatctctc gtacgcctcc cgcgcgaaac
     8941 ccgcctttgt ctcccattgg agcgcccctg attccctcat ctccaggccg tccatccagg
     9001 ggttcgcggc accccaggta tgctcgtcga agagcgccat gttctcgtat gtctcgtcta
     9061 tctcatcccg cgggtcggcg ccgtcttcgg caagcgtgtc ggccagggag tgcagggtct
     9121 gggccgtcct tatgtcagcc tgggcgcgac ggttgaatcc gaggtggcgc gcccccgacc
     9181 ctatgccgtc gacccaccag tcggtccagt cgccggagta ggtctccata ctccccgcaa
     9241 gcctctcctc ggccgaggtg aaaaactcgc tgttggtcgc caggcgtagt tgcgggtagg
     9301 cccacttctc gttccattcc cgcacgatct cagcggggac tatgctagga gccgcgttgt
     9361 cagagaatac gccctggacc ctcaggtgaa gcacatcgtg aggatacggc actttcgtca
     9421 cctcggatga gggatccagg cccgtccatc cgaagatgct ctccccacgc ccccggtacg
     9481 ggtagggacg ctttgagagg gccgcaagat agccaggcaa agcgtgcagg gtggcccggt
     9541 aatcctccgc cagaccgacc aggttgccct ccatgtaagc gctgccgtga ggcgagtccg
     9601 tgtaccacac caacaggcgt ttgccgttgg gggcgcgcca gtagaaggga cgcgtgagcc
     9661 cctgtccgcc gaccagatgc ggggcggaac gacccgcata attgtgggct acagaaaggt
     9721 agcgaacatc cgcgtccacc agggaactca ggaggcctat cgtggcccct ggcacgtcgg
     9781 tctgcatggc ggtccgtatg ggcacgtcgt acttctcgcg cagttcgtcg gcgaagcgta
     9841 gctggcgtgc cagttcgtcg atcgaatacg cttcggtatg catgctgaag ggaagcgcgc
     9901 agatctctat acgtccctcg cgaacacggt cgaagaaacg ggtcctgtca ctctctggcc
     9961 ggttctttaa ccagtggagg agtggccagt tggcttccac gttccaacgg aatgtggcgt
    10021 cctccggcca gtcatcggtg gccgaggtca gatccaggac cgagtcaaga tactgcaggt
    10081 ggtgctgtag caccgaactc tgggtgtccg tgtagccgat gtcgaggtgt gagtggtgga
    10141 cgaggaagac cgaccatttg cgctggggaa gaatctccac ccgcgcctcg aacggctccc
    10201 tgctcgtggc ctcgaccctg aggctaatcg tgcggggctc gtaaacctcg ggcacgaaga
    10261 gatgcacgag ggtgcggccc tcgtcgatcc tggcccttac cgtatcgagg gtggtatccc
    10321 cgtcggagat tgcgaaggtt acgtccccaa ccgcggattc ggccagcacc accagccgca
    10381 aggcctgcga gagatcgccg ttccccgtcc tgcggaagag tggcagactt ccgaccctca
    10441 cggagaagtc gtggacgtcg aagtccacgg cggaaggacc ctggatcagc tcctccctcg
    10501 ctctttgcgt ccttagatcg tcgcttgagg ggcgtttgcg gggcatggtg ctccttctct
    10561 tagcatttca gcttgccagc acgctccagc cttcggctgt cgttcgtcag cacttcagct
    10621 ggtcagcact tcagctcttc gcttgtggcc cgaaagatct cgtggtgctc gccatggtcc
    10681 cgaagacggt catcgtagaa ggagaatatt agggagttat gcccgacaac tcaattatcg
    10741 agtagcatgg agcgtgcgtg agccgttctc gcgtcgtgga ggagtcctct ggttaagatc
    10801 cactcctcat cggtctctcc ctgcggattt cattcgccgc gaggagaccc ggactcgttg
    10861 gtgtatagct tgagctctat caggtagccg tccgggtccc gcacgtacag ggcttcacct
    10921 gatcctctgg cgcctatgac gttcacagac ctgctttcga tctccacgcc cgcttccgtg
    10981 aggctttcgg ccagcgcatc cgggctcgtg ccttcgacca cgagggcgag gtgatcgtaa
    11041 cccccggtgg agacgggctc gaaggtggga tccggcctca gatgcagtat gaatccaggc
    11101 gtcacccgca gcgagacgag gggatgctcc ccgcgttcat aggcgtcgag gccgaacggc
    11161 tcgagaccca gaacgtcgcg atagaatccg agagacctct cgatatcctt tacccgccag
    11221 ttggtgtggt cagcttcggt tatcttcatc gtctttgtgt gtcctttcgt agtgagtccc
    11281 gcaaggccgt tggtcagggg tgtcggccgc gacgagcgcc tccggcccgc cgaactcctc
    11341 ccaccacacc gctatcataa tcgttagcta cctttcggtt cgttgcgtgt ggacccggac
    11401 aggttcttcg ccgggacggg ctccgggtgg tccagggggt gggccagctc gtcgcggtcg
    11461 agcctgggga tcgcgatggc cgcgacagcg aggaagacgg gcacgatccc ggcgacgacg
    11521 aagacggcgg tgatcccgaa agcggcgcct gccgggccgg cgatagccat ggagaccggc
    11581 atcagggcca gcgagacgaa gaagtcgagg ctcgagacgc ggccgcgcag gccttcgggc
    11641 acccggcgct ggagcagggt gccccagatc accatcgcgg cggagtaggt cgcgcctacg
    11701 actagcaagg cggcgcacat cacccacagc gcgccagcca cgccgaggac ggcgaaaggc
    11761 agggagccga gaccccacat gaggatcatg accgtgagat agcggcgcgg caaccgtccc
    11821 gaggagatcg ccagggcccc tacggcccct cccaccccga aggcggccag cgccagaccg
    11881 tagtcctcgg ccccgccccc aaggttgtcg cggatggcga aaggcagcag cacctccaat
    11941 ggcccgataa tgaacaggac gagggccagc gcgaacaaga gcgtcgccca cagccagctc
    12001 gtccttcgca cgtagcggaa gccgtcgcgc aagtcggaga agaccaccgc gacgccgggg
    12061 gcgccgttcg tacccggaac tttcctcggc cgcaccttca ttaccagcag gcatccggcg
    12121 gagaccagat aggccaggcc tccggtgagg atcgcgacac cgggcgagag cgccgccacc
    12181 gcgacgccgc ccagcgctgg ccccgccgct tgctgggcca gcggtctcaa ggtcccctcc
    12241 aggccgttcg ccgcgagcag ctcgtcctcg gggagcaggt gaggcagcag tgcggtgtag
    12301 gatgggatga aaaacgcttc gccggccccg atcagcactc cgcccacggc caggtgccag
    12361 atcctgaggt cgccggagag cgagagcagc ccgaggatga ccaggacagc ggcgcggatc
    12421 aggtcggcgg cgagcatgac gagccgctgg ggcagtcggt cggccgctac cccaccgatc
    12481 agcacgaacg cgagcaggcc gacgctgaac agggcggtaa ccagcgaaag ctcagtgggg
    12541 ccgccgccca gtgcgatcac ctgccacgcc aacgcgacca gccacagccc gcttccgacc
    12601 aacgagatcg ccagtcccgt ccaaagcagg cggaagtcgc ggtggcgcag gggacgaaag
    12661 gccgtgggca ccccgcccag cacggttacg atcccaccgg cccgaaccgc gtcgcccgta
    12721 ctatcctcat accttcaact ctactctatc ccgccctcta aaccagaccg tttcgcctct
    12781 tcctacgctc gtagttcatg cggaacagct ccagggctcc cgccacatca tccacgctct
    12841 cgaccctgta gctcgccgtc gtccggctgt ccggaaacgc gggatgaggg atcgccctcc
    12901 ccgtgcggat caactcgtcc ctgacctctc tcgggaaccg gaagtcggcg tggccgtcgt
    12961 cgtggatgtg cccgatctgg ggccctccac tctcgggatc tccgtacctg tagccggtga
    13021 cgccgatccc accaggaccg tgctcatccc tcttcttgaa gattccatcc catcccaaga
    13081 cctcgcgctc tatcagggcc aatggttcat cgttcatctg tcgctacctc actctgttac
    13141 actgcgcgtc tccgtgcgaa ggacttgaga acccggccgc cgtaccggct cagttcccac
    13201 cacagaaccg ggatccaccg gtagcttctt gtgctacctt tacgcagcgt cccggccagc
    13261 cgcctggtct ccgcttcgcg cctcagatcc tctctgcgct gccgccaggt ctgaatcatc
    13321 gcccaatcat tcaacccgga cctcctatcc ttagcctgct gtgcacgccc acatcttgcg
    13381 ccgggaagga gactcgcaca tcgggcggga ggacggtttt cggcctgaac ctttgtacag
    13441 tcttggcggc agcgaatccg atcagaggcg cgccgggcgc tcgcccgatc tacgctcctc
    13501 ttcctgcctt ccgacccatc atgcgcttcg cgcgatgatc aggaatcgat ggctactgac
    13561 ctgcaagggt gttccgctct ccatcaggca tccaaggccg gcaaacggtg tctggtgccg
    13621 gaagttcgat caatccatgt gccactgcag ggggaggagg taggggaagt cggtggtggg
    13681 gtcgatctct actttcatga tggggtccat ccaatcgctc caccgctggt tggtctcgct
    13741 gttcgccagg tactcccggg tcttgtcgat gtcgtcggtc tcgaagtagc cgaagagggt
    13801 caggccgtgg cggaagatgg agtagttgct gacgccggcc tccctgagcg cctcgaccat
    13861 ctccggccag atctcctggt ggcggcgcaa gtactcttct tcataccccg gtctgacctc
    13921 cagaacccac gcataccggg tcatccaccc ctccttatct cagtaccgcc actgcgcctc
    13981 atgatataac gctccggaac ccgtcggcgt atatgtcacc ctgagccttg cccgagagac
    14041 gaagtccagc aattttgtgg tgtttgaggc tagtttcatg cgtcgtatcc acgatcttgc
    14101 cgaatcgttc ggtcttcaac gcgtcatgcc ctacatggtt aggattgcgc gatgatgcac
    14161 gtttcgtgca ctcgcgagga acccgtttca caacatgctg ggagattctc ctaggtccgg
    14221 aaagactggc gcgcccagct gctgatgttt tctgttctcc caatcttcgc gtcgagtgca
    14281 cgcaggtgca tttggatgtt ccagtctcta gcaacattag cggcttgcaa tacttcccgc
    14341 ctgtgctcct cgagtatcgt ttccttgttc ccatgtacat cccaaagctc tttgacacga
    14401 gagaacgttt caggtgagac agacgtcccc agacccgagc cagccaaccg agagatcaca
    14461 tcggaatgcg aggtgggatc cattacatcc accagttgcg gttgtaagag taccaggaac
    14521 gcttggagcg aagcagccgt tgccttgagc tggttcgaga gcaacgaatg gcgctccatc
    14581 ccctgctctt ctaatggttc aatgatcttg cgcgactcct cctccaggat agggctgtgc
    14641 gcgttgtagt ggcccctcaa gaatacgagt cttgttgaga cctcatccgc ttgctggagg
    14701 agttccgcga gtccttctga aaaccccacc acccgttcaa cccccgcttt gggtgggact
    14761 tcgagattcg ctgccctatc agtccaggat cggaaggcgt tagcgaagta caatgcctga
    14821 atcctaatgt cgtcgagtgc ttcggcacgg cgttcgatta gcctttggtt cctctcctcc
    14881 tctcgttggt gctctcgttc cttgttctcc tctatcctgc tctgttcctt tcttcgccac
    14941 tcctgtagcc tgcgctgttc ctctccttgg ctggcaagaa ttcgcttgcg tttctctcgc
    15001 accccaccaa gatagaaggc ggcgatggcg ccaaccaacg ccccaacaca acctgccgcg
    15061 accattatga gaaacgcatc caccttctac acctcaatcg tagggaacat ggccagagta
    15121 ttcatcgaag gctaccgaga cctggtaccg cagtcgtaga gcagcacggt ggaggttggt
    15181 gatttggagg tcgacgactg ccacagtccc tcgggaacct gctcgcagtt gtccgttatc
    15241 cacctcacct gcttcttttc gtggatgtcc gccagggctt tgaggtgcgc cttgaggcgc
    15301 ggcagctgtt gtaggggcgg cacctgttcg tctagctcta ggtagcgccg gtcagtgccc
    15361 ggctgtgctg catgctcgat gtcacgctcc tttattatga agaaccgcac gggaccttgg
    15421 tgtacgagac ctgcgaccct ctcgatgcta aagaccaggt cgcgccccgc gaatccgctg
    15481 aacgctatta caggctcgtc tgtgttcaaa attatggggc tggcaatccg ggagcgtata
    15541 gcagcgacca ggtactcggt atcaccctgg ttggcttcca ggttttccac caggggatcc
    15601 acctcgct
//
LOCUS       DATMVF010000216        55941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_670297, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000216 DATMVF010000000
VERSION     DATMVF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 55941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55941
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670297"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VN178_10925"
     CDS             <1..579
                     /locus_tag="VN178_10925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWS81527.1"
                     /translation="KTRKGETVVEADEGPRRDTSVEKLGKLKPAFKGDGSVTPGNSSS
                     LNDGAAAVLVVSREYAEAHGLEPLAKVRSIAVAGVPPRVMGIGPVPATKKALMRAGIS
                     LEEVSLIELNEAFAAQSLAVLHEWSMDPEDERLNPNGGAIALGHPLGASGARILTTLL
                     HEMRRRDGVRYGLATMCIGVGQGIAMVVEKPA"
     gene            593..1315
                     /gene="pcaH"
                     /locus_tag="VN178_10930"
     CDS             593..1315
                     /gene="pcaH"
                     /locus_tag="VN178_10930"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HWS81528.1"
                     /translation="MRQQPTDETGGLRPEDNGQPPYLYPDYVATRLRAPKEPLIILPK
                     TLSDTTGPAYGRGPIGELDNDLTRQHGGEPLGERIIVAGRVFDGDGRPVRKSLVEIWQ
                     ANAAGRYTHHLDQHPAPLDPNFSGAGRCLTDDEGRYRFVTVKPGAYPWKNHDNAWRPA
                     HIHFSLFGPAFRSRLITQMYFPGDPLFEQDPIFRSVRDREARQRMISTFDLETTQPEW
                     ALGYRFDIVLEGRTATPMENPR"
     gene            1312..1878
                     /gene="pcaG"
                     /locus_tag="VN178_10935"
     CDS             1312..1878
                     /gene="pcaG"
                     /locus_tag="VN178_10935"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HWS81529.1"
                     /translation="MSPEPTPSQTIGPFFHDALLDEKYSELVSLDHPEAIVIRGTVYD
                     GAGEAVPDAMVEIWQANREGRYDRSTDGRHDPPPDEDFSGFGRSGTDAGGVFSFLTVK
                     PGPVPGPDGQLQAPHVMVSVFARGLLKRLVTRIYFPDEEEANAVDPVLSSVEDQGLRR
                     TLIARDEGDALRFDIHLQGDGQTAFFAF"
     gene            1881..3230
                     /gene="pcaB"
                     /locus_tag="VN178_10940"
     CDS             1881..3230
                     /gene="pcaB"
                     /locus_tag="VN178_10940"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HWS81530.1"
                     /translation="MAGELFRPIFVPERFSEAVSGRAWIRAMLEAEGALALAQARAGL
                     IPRQAAETIVSCCDAESFVPEEIGREGRAQGNPVPPLVRALTRAVSEVSEDAAFHVHK
                     GATSQDIMDTAAMLVSRHALGLILAEIDGISAACARLADTYRDTMMPGRTLLQQALPT
                     TFGKKAAGWLVSVLEAGTRLREMRQSGLAVQLGGAAGTLASLGPAGTKVLGELARELE
                     LAEPVIGWHTDRVRISEIGSALSLVAGVLGKISQDVILMAQTEIGEVAEPAGEGRGGS
                     STLPHKRNPILSVIAAANVRRVLDLSHTLQAAMVGEHERAAGAWHSEWEALSDALALT
                     GGAAAAVREVTEGLEIYPEKMLENLDATGGLLLAENVVTVAAQHLGRLEAHDLIEEAS
                     HRALENGKPLREELLSGLGEILSEEEIDAALDPAHYLGSAGEFVDRALKRYREGEIK"
     gene            3227..4015
                     /gene="pcaD"
                     /locus_tag="VN178_10945"
     CDS             3227..4015
                     /gene="pcaD"
                     /locus_tag="VN178_10945"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HWS81531.1"
                     /translation="MNEGTIVELNHILEGPEDAPVLVLSNSLGSTLGMWDDQAPILRE
                     RFRLVRYDQRGHGGSPVPSGPYKIEDLGRDALALLDRLEIESASFCGLSIGGLVGMWL
                     ASEAPERVERLVVCCTAARFEPEMYDARASKVRAEGVDSIADVVLERWFTPEFRAARP
                     ETVESAGSMLRSTPAEGYAGCCEALRDADVRDRLGEIRAPTLVIAGAEDPAATVGQAE
                     ELCDSIPEARLVVVGAAHLANIEQPEAVTREILEHLEPALGDGR"
     gene            4012..4401
                     /gene="pcaC"
                     /locus_tag="VN178_10950"
     CDS             4012..4401
                     /gene="pcaC"
                     /locus_tag="VN178_10950"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HWS81532.1"
                     /translation="MTPEDSTYDRGMKVRREVLGDEYVDRTIGRTTEFTADFQDFITR
                     YAWGEIWSRPGLDRKTRSCITLTALVALGRLDELELHVRAAVRNGLTEQEIGEVFLQC
                     AVYCGAPAANSAFAVAQRVFSETGKGE"
     gene            4402..5592
                     /locus_tag="VN178_10955"
     CDS             4402..5592
                     /locus_tag="VN178_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HWS81533.1"
                     /translation="MRTQVGIVGGGPAGLLLSHLLHLQGIESVVLERRSREDLETTVR
                     AGVLEYGTAGLLSEVGVGERMEQEGSVHHGIELRFDGRGHRIDFADLTDGLSITIYGQ
                     QEVVKDLIRARLEADGRILFEAGAESVEAPDTDSPKIRFWQDGEQEEISCDYVAGCDG
                     SFGVCRSGIPEDARTEYTRTYPFGWFGILVEGPPSTQELIYTLHDRGFALVSTRSPEV
                     QRLYFQCDPQDDVEEWPDERIWDELSTRLATHDGWELAGGRIFQKDIVQMRSFVCEPM
                     QHGRLFLAGDAAHIVPPTGAKGMNLAVADIRVLAHALTEFYRSGDWRLLDAYSETCLR
                     RVWRAQRFSWWMTSMLHRFDRSDAFQLKVQQAELDYITTSRAAATTIAENYVGAALGT
                     SHPL"
     gene            5601..6434
                     /locus_tag="VN178_10960"
     CDS             5601..6434
                     /locus_tag="VN178_10960"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWS81534.1"
                     /translation="MSRGRISGKTRLLALIGHPVGHSLSPAMHNAAFAADGLDFVYVC
                     LDVDPHDLPAAVRGLAASKLRGFNVTMPHKRAMIPLMNELDESARVSGAVNTVVIDAS
                     EMRGFNTDGGGMVMACQEAGIELSGRNILLLGAGGTAAAIAVAFGKAGAGELHIANRS
                     VERAAHLRDKLYRTGMKGLAVHHLNALPDAEIVINTTPLGMKEGDPIPVPPDYVQEGR
                     AFCDAVYRPGTETPLVRLARERGIPVVAGGRMLLYQGVLAQKLWTGREPNVKAMDRAI
                     S"
     gene            complement(6581..7339)
                     /locus_tag="VN178_10965"
     CDS             complement(6581..7339)
                     /locus_tag="VN178_10965"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81535.1"
                     /translation="MGVIDGWLEGFLHGQVGVGVILLVSLLLGLRHASDPDHLAAVTT
                     LIASEKERDQVRKAGAMGFLWGLGHGTTLVLIGLPLVLLNQYLPEVISKVAEVAIGCI
                     IVLLAVRLLIRWRRGFYHVHVHTHDGGEAHRHVHCHAHDESHGHVHRVPRHTPLSSYG
                     IGLVHGIGGSGGLTLLLLSTISEKAQATGALILFAAGTAVSMALLSTAFGLIIAGGPI
                     ARNFERVAPVLGVLSMAFGAWYALGALGVVVYPF"
     gene            complement(7375..7569)
                     /locus_tag="VN178_10970"
     CDS             complement(7375..7569)
                     /locus_tag="VN178_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81536.1"
                     /translation="MLVTDYVGGVVGLQAVMGVLGGQESTLGVVASTLTIAALFNPLR
                     SRVQAFVDQRFYRRKYDASW"
     gene            7816..8274
                     /locus_tag="VN178_10975"
     CDS             7816..8274
                     /locus_tag="VN178_10975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWS81537.1"
                     /translation="MEESEQKIVESKEIRARYPHFLRVPTRWMDNDVYGHVNNVIYYS
                     YFDTVVNEYLIRSGVLDIERSPVIGLVVETQCLFFEPLTFPDTVHAGLRVARLGRSSV
                     RYEIGLFRNDGEIAAAQGHFVHVYVERVSRKATPLPPGMRAALEKILASQ"
     gene            complement(8278..8766)
                     /locus_tag="VN178_10980"
     CDS             complement(8278..8766)
                     /locus_tag="VN178_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWS81538.1"
                     /translation="MSEKTGLREDLRSEVEALVASDASDEEVLNEVVRKVHEAHPAWD
                     WSGIYLLVGDTLILGPRTTPVDHSRIGIGEGVCGTAVSEGRNQIVEDVREVENYLACS
                     IYTRSELVVLIRDAGRIVGEFDIDSDTVGAFSQEDEALLEEMGALISGHVASLVGSYE
                     SN"
     gene            complement(8894..9118)
                     /locus_tag="VN178_10985"
     CDS             complement(8894..9118)
                     /locus_tag="VN178_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81539.1"
                     /translation="MTEGQREAPDRPEAERYYAQHASLEAGESGSSEAALQEAINEGA
                     RQSWKLISVTQDPTSGGVLLVWDTSGFFSG"
     gene            9509..9982
                     /locus_tag="VN178_10990"
     CDS             9509..9982
                     /locus_tag="VN178_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWS81540.1"
                     /translation="MTVDGNAHVAEVEPRLLLLHHLRENLGIGGVHTGCNTTSCGACT
                     VHIDGESVKSCTVLAVQADGHEITTVRGITENGVWHPMQQAFHEAHGLQCGYCTPGMI
                     MASISLLKENPDATEQEIREGLEGNLCRCTGYENIVKAVQLVQQHEDEMAQHAAS"
     gene            9996..12563
                     /locus_tag="VN178_10995"
     CDS             9996..12563
                     /locus_tag="VN178_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWS81541.1"
                     /translation="MTQAPEKFVGGGVLRKEDPALVTGRATWTDNITLPGMLHAVFMR
                     SPYAHAKITSIDVSAAAEQPGVVAVFTGEDLIDEYEKHPRENPAAVTGYDLSLHDRRQ
                     GHTVTGETEGGDGQGADEPRSFVAWIVHDELKIPHHDPLAHDEVNFAGEPVAVVVATD
                     RYKAQDAVEFIEVDYEPLDVVTDLEEAMTEGSPLAHENFGTNEAYTWDLATGDIDEAF
                     SKADVTVKGRYLQPRLIPNAIETRGVVASPDPVSGGFTVYTSTQVPHITKAVLSSYLG
                     VPEQKLRLVAPDVGGGFGSKLNVYAEEVIALVLARRMGVPIKWIEDRSENYLATIHGR
                     GQVQDIEVAADSEGKILGMKVNLLADMGAYLHLNTAGIPLLGAFMYPGLYTFPAYYFE
                     CIGVYTNKTPTDAYRGAGRPEAAFAVERIMDQLAAEVGIDPAEVRRRNFYEPFDEPTD
                     TPAGIQYDSGNYQMTLDRVIELADYEGLREEQRRRRESGDPVQLGIGFSTYTEICGLA
                     PSQVLTALGAGGAGWEMAKVQMLASGKVEVITGTTPHGQGHVTSWSQIAADVLGVTPD
                     DVEVLHGDTAIAPYGRDTYGSRSLPVGGVAVHMAAQKVVEKARKIAAHMLEAAEDDIE
                     FEGGTFSVAGSPDQNVTMADVSGAAYLANNLPEGMQPVLSEEFFFDPPNFTFPFGAHI
                     CVTEVDTETGFVKIRDYFGVDDCGPVINPTIVDGQLHGGIAQGVAQALYEEAVYDEEG
                     NLTTGSMVDYLIPGAPELPNYTLERTVTPSTSNEMGVKGVGEAGTIGAPPAVINSIVD
                     ALSPYGITHVDMPASPWKVWQAIQDAQGKEAGTRDADRSGRPSQAVDSTDTEGGSVS"
     gene            12560..13408
                     /locus_tag="VN178_11000"
     CDS             12560..13408
                     /locus_tag="VN178_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWS81542.1"
                     /translation="MIPLAFDYEVAESVDHAIELLGQHGEEAKLLAGGHSLLPIMKLR
                     LAAPTVLVDLGRIDDLNYVRDEGDHLAIGAMTRHTDMERDATLKEHCGLIAYTASLVG
                     DPQVRHRGTIGGSISHGDAASDLPSALLALDATFVVKGPGGERSVAAGDFFEDYLQTT
                     LAPDEVLTEIRVPKLGGAGWSYKKFNRRAQDWAVVGVAAVVERSNGSIGSARIGLTNM
                     GSTPLRATAAENALSGADPSSVAEATSSADEGTSPSSDIAASDEYRRHLARVLSRRAV
                     EEALER"
     gene            13405..14349
                     /locus_tag="VN178_11005"
     CDS             13405..14349
                     /locus_tag="VN178_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWS81543.1"
                     /translation="MTEASDGRRGALPPGVSHFRELGARLREHDYLADEGLSTAIFLS
                     LKLGRPLFLEGEAGVGKTEVAKVLSRILKTPLIRLQCYEGIDVYQAVYEWDYARQLLH
                     IRAAEAFGGEDRERVERDLYDREFLTSRPLLQALEHRGPMPPVLLIDEVDRSDDEFEA
                     FLLEILSDFAVTIPELGTVMAERPPVAILTSNRTREVHDALKRRCLYHYIEHPSFERE
                     VEIVRLRVPDAEERLARQVAAATEKMRTMDLYKPPGVAETLDWTEALVALGAKELDEQ
                     LVEATLGSVLKYREDQQRSANSGLAALVSGVGGAGRDA"
     gene            14342..15484
                     /locus_tag="VN178_11010"
     CDS             14342..15484
                     /locus_tag="VN178_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWS81544.1"
                     /translation="MPEGEIQAPPMLDAAVAFGRVLRGAGLRVGTDRLVEFARAVEEM
                     DPSRRDDVYWSGRITLTSRPEDIEIYDRAFELFWETGGGAKPRPRPKVRLSVPQPDRS
                     VMPPKKTVEKSESGEEAVRLRYSPVEVLRRKDFALFSPEEFAELQRLLADLRLSGALR
                     KSRRLEPAPRGRHDPRRTLRGAMRTGGEAVRHRFRKARVQPRRVVLLCDVSGSMAPYS
                     RALLRFLHASVVAGGRLEAFSLGTRLTRITRELTTRDPDRALRQTAGAVKDISGGTRL
                     GDAIKEFVDRWGQRGMARGAVVVIFSDGWDRGDVSVLAEQMQRLSRLAYRVVWVNPLK
                     AAPGYKPLAAGMAAALPHVDVFLSGHNFESLEELAHAVAAATERER"
     gene            15708..16022
                     /locus_tag="VN178_11015"
     CDS             15708..16022
                     /locus_tag="VN178_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWS81545.1"
                     /translation="MNPVIDKVAQWSEEGKQAALATVVKVERSGPLGPGTSMAVSEDG
                     QVVGSVSGGCVEPAVYEEAMSAIEGGEPRLLSYGISDDEAFGVGLTCGGTIHIFVERV
                     DW"
     gene            16022..16840
                     /locus_tag="VN178_11020"
     CDS             16022..16840
                     /locus_tag="VN178_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HWS81546.1"
                     /translation="MADLLSRWHAALSEQSPVAVATVIQGPEGYLGGKILVSPEDHTG
                     TAGNVELDKAVVEAARGLLEGGRTETVHLGPRGQRRMEDVAVFIQSFAPPPRMYVFGA
                     IDFASAVARAGKFMGYRVIVCDARAVFATRERFPSADEIVVAWPDEYLKTAEVDGRSV
                     IAVLTHDPKFDVPVLKEALSTPAGYIGAMGSRRTHDNRTARLKEEGVTDEQLARISSP
                     IGLDIGARTPEETAIAIAAEIVALRTGHSGGRLAERSGPIHSAAPDTRTRDGVK"
     gene            16919..17794
                     /locus_tag="VN178_11025"
     CDS             16919..17794
                     /locus_tag="VN178_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWS81547.1"
                     /translation="MQVPARFDYEVAGSVGEAVQLLERYGEGARLIAGGHSLIPMMKL
                     RLAAPEVVIDISGLESELRYIEDGDGALRIGALTRHREVLDSPVVREWYALLADAERM
                     IADPLVRNMGTIGGDLANADPAEDLPAAFVALDSEVLVRGPDGGRTLHIDDLYVGPYE
                     TSLDHGEVLTEIRVPRAPGGSAYSKVKRKTGDYAAAAIGVALSMEGEEIESIRIGLCG
                     VGSRTLRARGAEEALTGERPGSEAYRRAGEIAAGECDPIDDARGSVEYKRDLVRVLLG
                     RTVDKAVERAAGREV"
     gene            17797..18276
                     /locus_tag="VN178_11030"
     CDS             17797..18276
                     /locus_tag="VN178_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWS81548.1"
                     /translation="MRINVNVNGAMLEAEVEPRLLLVHFIRETLGLTGTHWGCDTSNC
                     GACTVLLDGEPVKSCTVLAVRADSHEVTTIEGLWSGTDLTPVQEGFHVEHGLQCGFCT
                     PGMILTATEFLERNPDPTEAEIREAISGNLCRCTGYENIVKAVQWAARNGSAQEVGA"
     gene            18276..20627
                     /locus_tag="VN178_11035"
     CDS             18276..20627
                     /locus_tag="VN178_11035"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HWS81549.1"
                     /translation="MATVQGHTLGDRMPRKEDGRFIRGKGNYVDDVRLTGMLHSALLR
                     SPYAHARINSVDTSAALELSGVHAVITGDDLAEQGLAWMPTLAGDTQAVLATDKVRFQ
                     GQEVAFVVADSTYIAQDALELIDVDYEPLDPVIDSRKALDDGVPVIRDDKEGKDDNSI
                     FFWDAGDKEATGKAFEEAEVKVRQEMYYPRIHPAPMETCGTVADYDVSTGEMTIHLTS
                     QAPHAHRTLFAIVSGLPEHQIRIISPDIGGGFGNKVPIYPGYVCAAVASLVLGRPIKW
                     METRSENLMNSSFARDYHMVGELAATSEGKILGARSSVLANHGAFDAHAQPGEQYPTG
                     FFPIFTSCYDVPAAYAEATGVYTNRAPGGIAYRCSFRVTEAIYLMERTMDVLADELGM
                     DPVELRRKNFVTKEQMPYVSVMGWEYDGGDYEGALQEALRIAGYEELLEEQRQAREEG
                     RIMGIGLSTFTEIVGAGPGKKCHIAGIEMFDAAELRIHPTGKAILKLGVKSQGQGHET
                     TFAQIVAGELGIPAENIEVREGDTDNTPFGLGTYGSRSTPVCGAATAVVTRKVREKAK
                     KIAAHLLEAAEGDVEYEDGRFYVKGSPDQAKTMDEVAFAAYTNVPEGEEAGLEAVTYY
                     DPPEMTYPFGAYICVVEIDRGTGQVEVKRFIAVDDCGVRINPTVVDGQVHGGLAQGIG
                     TALMEIIDFDEEGNHLNSSFMDYLIPTSMEVPNYELGEFVTPSTHHPLGARGVAESPT
                     VGSPPAVVNAVVDALSPFGVRNVDMPLTPNKIWKLMREHGVEA"
     gene            20633..21604
                     /locus_tag="VN178_11040"
     CDS             20633..21604
                     /locus_tag="VN178_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWS81550.1"
                     /translation="MLRQDPNSLARRAESLRRAGDLAGGERPFALVMVVRTEPATSAR
                     IGDRAIVFADGSLDGWVGGGCIGPTARKEALAALEEGVTRLVRVTPDTAAAQPGVRMA
                     RMTCASEGTADLYVEPFLPRPTLIVAGDSPVVATLTAIAPPLGFRLLTMDSTTRLTPG
                     EVPYPQDSWMVVATFGEFDEDAVEAGIRLGLPYVGLVASAQRAEQVLSELRTRGLEEG
                     ELAVVRSPAGLPLGTSGQEGISLSVMAEISSLRAEARPSFSGVQERESAGEPDTAVDP
                     VCGMAVEMASARHVVEQGGTRYYFCCAGCRRTFEQRPEKYETRVSLR"
     gene            21601..22197
                     /locus_tag="VN178_11045"
     CDS             21601..22197
                     /locus_tag="VN178_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWS81551.1"
                     /translation="MSVVGVVLGAGRSSRFGAPKQLLPFGDTTLLGQAVGNANASSLD
                     RTVVVLGRASGELRGAIDFGRAEVVENTAYGTGCASSLLAGLDAAGDDCDALFLLLGD
                     QPGVLPGFIDLALAEWRDERPWAAVCSYRGRLGHPFVFSLDAFGDLRGLHGDKAVWKL
                     IEAYPDRVQEIELDAELPPDVDTPEDYERALIHHREAS"
     gene            22197..22823
                     /locus_tag="VN178_11050"
     CDS             22197..22823
                     /locus_tag="VN178_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWS81552.1"
                     /translation="MLRPSGVSAIVLAAGGGSRFGGGKLLAELGGRPIIETVLHNLRE
                     APVDEIIVVLGADAQRLREVCERYGARTLANEEWERGQSTSVLAGLRACGGEAAVVLL
                     GDQPFIGAEAVERLVSAFSEGAKVAVATYGGKRRNPVLFSREVWPLLEAELAGDEGAR
                     SVQRRHPELVVEVPCEEVGDPADIDTREDLKRLEEMRGPMGDYKMAGG"
     gene            22874..23701
                     /locus_tag="VN178_11055"
     CDS             22874..23701
                     /locus_tag="VN178_11055"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWS81553.1"
                     /translation="MKINNEFTVAAPIQQAWDTMLNLERIAPCLPGAAIQEEKDEGEY
                     DGTMKVKIGPITANYKGTVKFEEVDEGNHRAVLQATGRDARGQGTASATIVSTLQEEG
                     EGTKVSVETDMKLTGRAAQFGRGIAQDVATKMLDQFASCLEEEITGGPEEGAAAATSE
                     PAGEENGSRDGAQQEEAPPAAAAGGTAGRVISSEDPTVMAGGTVEGAVVAGSAEAPTG
                     APTAGTEQQPPRQEREEPEAFDVGAASQEAILKRAKPVLIGAGVLLVLIWLLRRRRR"
     gene            23959..24795
                     /locus_tag="VN178_11060"
     CDS             23959..24795
                     /locus_tag="VN178_11060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWS81554.1"
                     /translation="MRYGFLIPTGDPRTVAELARGVEEAGWDGAFYWDGICVGDMETY
                     DPWVVMSGMAMRTERVRIGAVLTPPARRRPWKLARETVALDHLSNGRLVLPVGLGALD
                     DGGFSKVGEPTDRKVRAQLLDESLDILTGLWSGEPFSYAGEHYRLEEMTFSPPPVQEP
                     RIPIWVVGAWPSRKSMGRTLRFDGVLAAAVGGGPESPGVTPETIREIRDYVRENREDD
                     GTFDIIWEGQTPGEDPGRAASIVRPFAQAGATWWIESPWMPPNEPDDLRRRIEQGPPS
                     VD"
     gene            complement(24861..25073)
                     /locus_tag="VN178_11065"
     CDS             complement(24861..25073)
                     /locus_tag="VN178_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81555.1"
                     /translation="MWSRLTRGGDGLPREKVDAIVRLIDRYLAEEASLRNLQSDKQTI
                     HPRDLPPDKREALIEEVFEILKDARR"
     gene            complement(25135..26001)
                     /locus_tag="VN178_11070"
     CDS             complement(25135..26001)
                     /locus_tag="VN178_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWS81556.1"
                     /translation="MATVVHISDLHFGRPAVAERLDALKGYVEMIQPDAVAISGDLTQ
                     RCTNGEFSNARAYLDSLGKIAPYTVVPGNHDIRWLGAVARNLGLAGLFREQAHKFKYS
                     RYVRHISEDLSPSLEVPGVVIAGVNTAHGITRGSITRRLRDLGVIGQVRHADVMRVRG
                     IFEHAAPTSARIVMMHHNPIRGEQSGRHGLANTEQALHAFADLGTELILCGHDHQDAI
                     HTIERGAHGLVISTAGTISNRIRPGRASSFNVVEIEDHEIHITAHTWRQGSGFIPSED
                     RAFPRRTASRRA"
     gene            complement(26076..26867)
                     /locus_tag="VN178_11075"
     CDS             complement(26076..26867)
                     /locus_tag="VN178_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS81557.1"
                     /translation="MDHRTSGVAMSYRDLGEGDPLVLLHAFPLNGRMFEPQMGAFSGS
                     HRVVAPDYPGFGRSPRTPAQPDVHYYAEGVRGLLDRLHLERVILGGVSMGGYVAFECM
                     RLFPERVRALILANTRAEPDSEEIRETRNETALRVAEEGVGVLVELQMERLLAPDTLR
                     NDEELVENVRAMIFENSPDGVVAALGAMRERPDSTPLLGKIGVPTLVVGGEEDGISSP
                     EVMGAMAAKIPEARHVTIPRVGHLSNLEAPEKFDAVLADFLQSIG"
     gene            27076..27687
                     /locus_tag="VN178_11080"
     CDS             27076..27687
                     /locus_tag="VN178_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS81558.1"
                     /translation="MWKNLASVMEGEIVRLEPLAHRHEEGLFEVAQDGRIWRWMPYDP
                     SGSRESFRSWLGDALAASSAGTEAAFATVNVATGEPVGSTRYLALRPEHRGLEIGWTW
                     LAPARWQTGANVEAKLLMLDHAFGRLGCLRVEFKTDSRNERSRAALAALPARFEGIFR
                     KHMLVRDGERRDSAYYSIVDDEWPEVRENLSRRIDAIRKEEAT"
     gene            27684..28037
                     /locus_tag="VN178_11085"
     CDS             27684..28037
                     /locus_tag="VN178_11085"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS81559.1"
                     /translation="MSEGGTAAGRRRATVAEAMGRLPGPEGERFAEVLGHGSMEVEIY
                     APRGTDPQKPHTRDELYVVVSGSGTFVNGPERDPFGPGDVLFVPAGVEHRFEDFTDDL
                     AVWVVFYGPEGGEAG"
     gene            28034..28651
                     /locus_tag="VN178_11090"
     CDS             28034..28651
                     /locus_tag="VN178_11090"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWS81560.1"
                     /translation="MTMRLHDHLPSGNGYKVRLLMAQLGIPFERIEYDITEGETRTPE
                     FLENVNANGRIPVLETETGEFLPESNAILFYLAEGTPFLPDDRFDRAQALRWMFFEQY
                     NHEPNIAVARAWLHVFHTEMTEERLGALEVKQKLGYDALGVMEEHLEGRLFFVGGRYS
                     VADVALYAYTHVADEGGFDLEGFPAVSAWLERVSSQPGYIPITQG"
     gene            28686..29774
                     /locus_tag="VN178_11095"
     CDS             28686..29774
                     /locus_tag="VN178_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWS81561.1"
                     /translation="MTTELRTSHPYHMHEAILAQPEALIRVLERNESAIDEFATRVSL
                     CERVFLAGIGTSHHASLVGEHLMRSYGGGPDVRAFHSFDLALYGPELRREDCVVAVSH
                     RGAKRYTALALEQAREKGCPTALITGEAGSGEGRADAVFRTVAQESSPAHTVSYTTAV
                     ALLALLAGRVGYHRTGSATLPYGLLREELPGALRAALGTEGEVAAFAREHVGRRRIWL
                     LGGGPSAVTATETALKIKETSYLQAEGMPTETMLHGPFQCVEADDLFVLIAPSGAARE
                     RTLEVAELVEEVGAPYVIFGDDTTEGRPAAAGVLRVPEVPEPFSALTCLVPLQLFAYH
                     LALARGTNPDSFRGDDPRFARADISARL"
     gene            29790..30656
                     /locus_tag="VN178_11100"
     CDS             29790..30656
                     /locus_tag="VN178_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS81562.1"
                     /translation="MRLIYTLLLVATTAVWGWTFVVVQDAISVYGVIPFLAVRFALAG
                     AALAPIYAMKLTRRSLIVGGGIGVVLAAGYLFQTTGLLFTTPTNSGLITGLFVVFAPL
                     ADRFLFGANVSRQVVLAVALSLLGMVLLAGGGPEGANWGDLLTLLCAAALGLHIALLS
                     RYAASYDAGALTLAQILSIALLFVIVWPFFAPVSLPTPEVWFALLVTGLLASAGAFLV
                     QTTVQQHIPAARTAIILTMEPVFAALFGYWLAGDRLVAVQILGALMILSALVIGEVVP
                     VLRRGKSPVHHD"
     gene            30764..31429
                     /locus_tag="VN178_11105"
     CDS             30764..31429
                     /locus_tag="VN178_11105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWS81563.1"
                     /translation="MAHTVKVPEYEVQDGVRVDPSRTALIIGDMQNDFVTEGGTLVVP
                     GAEETIPAIRNLLELARESGMKVVFVQDTHNDGDPEWEIWGEHCREGSWGWQVVDGLA
                     PNEDEATIRKPRYDAFYGTGLEHMLRLWDVHTLIICGTVANICVHYTASSAAMRWFNV
                     IVPRDCISSFNDFDMEASLSQTAVLLGEVTSSENIHIGVPEPEEIYMSKLEEAATRER
                     GER"
     gene            complement(31530..32024)
                     /locus_tag="VN178_11110"
     CDS             complement(31530..32024)
                     /locus_tag="VN178_11110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS81564.1"
                     /translation="MEITIRHSEPEDYEALRRIFSGPKAIAGTLQMPLPSAETWRKRL
                     SEPPEGLYSLVACVEDEVVGSLGLETSPTRWRMRHVGGIGMAVRDDWQGKGVGTALME
                     AALDLTDNWLNLTRIELSVYVDNAPAIALYEKFGFEVEGTHRRFAFRKGEYVDAYSMA
                     RLKP"
     gene            complement(32115..33743)
                     /locus_tag="VN178_11115"
     CDS             complement(32115..33743)
                     /locus_tag="VN178_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HWS81565.1"
                     /translation="MSDQAISIIFFVLIIALTLGITAWASRQNKSTSDHYVAGGGIKG
                     WQNGLAISGDYLSAASFLGIAGAISLTGFSGFYLSIGFLVAYLVVLLLVAEPLRNLGK
                     YTLADMLASRFNKPSVRSAAALSTIAISMFYMIAQLNGAGALIELLLGLDYVLSVIII
                     GILMTVYIVAGGMVATTWIQIVKAVLLIAGTLTLSLFVLAQPSIGFNPVNLFNEVSSQ
                     IGSDMVKPPPPTDLVAGLDVISLNIALVLGTAGLPHILIRFLTVPDAKTARSSIVTAT
                     WIIGLFYLLTPVLGYGAALFVGQNAIAQQNPAGNTAAPQLAEALGGPIFFAFISAVAF
                     ATIVAVVAGLVVAGSSAFAHDFYTNVIRGGNASEQEQFRAARFTAVGISVAAILLAIP
                     AQNLNVAFLVALAFAVAASANVPVILLTIFWRRFNTTGAVTGILVGLISCVVLIILSP
                     NVMTGPNPEPPLTPIIPIDPIFPFVYPALFSVPLGILGCILGTLVGGRGAEREREQGL
                     QTDYDEIYVRSLTGISNIEQELQESAPAEEPRTT"
     gene            complement(33740..34084)
                     /locus_tag="VN178_11120"
     CDS             complement(33740..34084)
                     /locus_tag="VN178_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HWS81566.1"
                     /translation="MERHEEWVRIERTSAFRELTQKRKAFILPATIFFFVFYFGLPFL
                     TAFTTVLDVNVIGAINLAWIYAFAQFAMTWILMHLYVSRANGWDDLVDRARHEASEVD
                     EGGRTDGTGGSE"
     gene            complement(34289..36484)
                     /locus_tag="VN178_11125"
     CDS             complement(34289..36484)
                     /locus_tag="VN178_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(36038..36040),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWS81567.1"
                     /translation="MATAERVHENGKANGARELTTICGFCGVGCGLSVKVEGGVITKV
                     AGDKENPSSKGEACIKGREGWRHLYSDRRLTRPLVRKDGELVPASWDEALDLVAGKMS
                     EIKEEHGPDAFGLFSSSRSTNELNYLASRFVRQVLGNSNIDSCNRAUHAASVTGLVAT
                     FGAGGATTSYEELENTDLIICWGSNTMECHPIIYNHMRRGIKNGAKMVVVDPRKIDQT
                     KKAEKWLGLNVGTDIALANAMANVIIAEDLHDKEFIERATKDFEAYRDKVSGYTPEYA
                     EQITGVPADDIREVARMYATADTAVLNWTLGITEHHNGADGVFALIGLGLLTGHVGKY
                     GSGLNPLRGQNNVQGGGDMGALPDKMVGGWRWDDPEAHELFEEVWGSPLPEKVGKHQP
                     AMMDAVERGDIRCMYVIGENPAQSDADEHYHDRLFGGLDLLVVQDIMMTKTARMADVV
                     LPAAASWAEAEGTVVNSERRVQRVHRVVDPPGEARPDEWIVQDIANRMGYDWGFETVE
                     DVWEEIRRLAPKFSGMSYERLEQHGGIQWPCLDGSFEEISDNTPKEQLAASTRGAENA
                     KGEAITTDVVEKPHVGTKFLHAHLRAEEVENRAPFTPVDHEGPVEMPDEEYPFQLTTG
                     RRLAFHNTGTMTQDYKKVKDPEELLEICEEDAEGHGIEDGDMVRVSSRRGTVEAVKAR
                     VTDRVRPGLLFMGYSFPDQVPTNVLTINAVDPQSGTAELKACAVSIEKA"
     gene            36736..38031
                     /locus_tag="VN178_11130"
     CDS             36736..38031
                     /locus_tag="VN178_11130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWS81568.1"
                     /translation="MSIGEVYAFSVFKNPLVERFGVGLTPIGIIFSISIVMLGLSAAV
                     FGTWVERAGPRAAMFTAALCWSSGFAIGALGVATTQLWLVYLGYGVVGGIGLGIGYIS
                     PVSTLIKWFPDRPGLATGLAIMGFGGGALIASPLSSTLLETYASNPVDAIVPSFLTLG
                     ALYLVSMMIGAFVVRLPPEDWQPEGWTPPTEEETSDLQTTADVSASSSIRTPQFWMLW
                     TVLFCNVTAGIGVLEQASPMIQDFFSGITPAAAAGFVGLLSLANMAGRIGWSSTSDYI
                     GRKPIYMVYLGVGVVLYFVLAAFGAASLVLFLLVTMVILSFYGGGFATVPAYLKDLFG
                     VAQVGAIHGRLLTAWSAAGVAGPLIINAIADYQIANGRTGADLYTLSLYIMVGVLAVG
                     FVANLLVRPVDPRFYMEPGPEGEPEKESTASGRAAGSES"
     gene            38042..38191
                     /locus_tag="VN178_11135"
     CDS             38042..38191
                     /locus_tag="VN178_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81569.1"
                     /translation="MSEEARQENGGQTSSSSTILVVVLWAMVLIPLAWGIYQTLLGVV
                     ALFTG"
     gene            complement(38263..39123)
                     /gene="fdhD"
                     /locus_tag="VN178_11140"
     CDS             complement(38263..39123)
                     /gene="fdhD"
                     /locus_tag="VN178_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWS81570.1"
                     /translation="MLEKTSDRVRRGSKTKTRVRVVEDGRMRVRPDTLATEEPMEIRL
                     LAGQTTQTVAVTMRTPGADFELAAGFLYGEGIIDSPDDVRKISYCVDSDLDAEQRYNI
                     VNVELRGGREFDLRPLERHFYTTSACGVCGKASLEQLELRGCPVMTAGPEVSAEVLTT
                     LPEKLRAAQGLFDATGGLHAAALFDEEGELIALREDVGRHNAMDKLVGWALLEGRLPL
                     TDHIVMVSGRSSFEILQKSLAAGAPVVCAISAPSSLAVDVARQFGMTLVGFLRDCRFN
                     VYAGSERIRI"
     gene            39223..39444
                     /locus_tag="VN178_11145"
     CDS             39223..39444
                     /locus_tag="VN178_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81571.1"
                     /translation="MGLPNVARYPEATVARDETSILIHFAGPYGEQKMSVPLGYVGGD
                     AEAAELWLLAQLQEIGYSVRRGEPGPSAP"
     gene            39604..40671
                     /locus_tag="VN178_11150"
     CDS             39604..40671
                     /locus_tag="VN178_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS81572.1"
                     /translation="MPETPELSRRNTAVDLGESVEFPMTPGRTSPNDNLPLHLSSLIG
                     RERETAQAKRLLDHHRLLTLTGPGGSGKTRLALAVGEFEDGVWWVALASLSDPELVGQ
                     AVASVLEVREQPGRSLGETLADHLASRETLLILDNCEHLIEACARLADALPHSCSNLR
                     VLATSREALGVAGETVLLVPPLSLPDSGHPPPPEELERYEAIRLFVERARYASPSFEL
                     TGRNASAVARLCRNLDGIPLAIELAAARTRVLSVEQISSRLADSFRLLKSESRTADPR
                     QKTLEATLEWSHELLGEKERALFRRLSVFAGGFTLEAAEEVCAGGDIEEDAVLEVLSD
                     LVEVVGVGHRAGWGGGALPAP"
     gene            41136..41519
                     /locus_tag="VN178_11155"
     CDS             41136..41519
                     /locus_tag="VN178_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81573.1"
                     /translation="MKSPAREYVTLVEVLELTRPFEEFADAIEAASRRLEAEGVQELI
                     AVHFYGSPESTEAGAILTFSDRDRMIDHIEMISGWEEFERFFATVKPLDVRVYGRLSE
                     EAEEWIGRFDVLSKKFEDHVAGFVR"
     gene            complement(41557..42204)
                     /locus_tag="VN178_11160"
     CDS             complement(41557..42204)
                     /locus_tag="VN178_11160"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWS81574.1"
                     /translation="METVKTPAEQRVVLHNIGWNTYERLLADHENNSAPRFTYDRGEL
                     EIMSPSPEHEKFNRRIAQLVLAVAEELDIEAEDLGSTTFRREDLERGFEPDSCFYIQN
                     EEQVRGKERIDLAVDPPPDLVIEIDITSPSFNKLPIYAQIGVPEVWRYNGERMTILIL
                     EGSDYAETTESIVLPPVTNGVLTDFVEKSKTTRRTVWLQRVRNWAHKRSEPGAVR"
     gene            complement(42277..42591)
                     /locus_tag="VN178_11165"
                     /pseudo
     CDS             complement(42277..42591)
                     /locus_tag="VN178_11165"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
     gene            complement(43026..44234)
                     /gene="fdhA"
                     /locus_tag="VN178_11170"
     CDS             complement(43026..44234)
                     /gene="fdhA"
                     /locus_tag="VN178_11170"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR02819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HWS81575.1"
                     /translation="MADNRVVVYKGPGEVAVEDIEYPKLEVPEEVADAMGMTKAAPHA
                     VILRIVATNICGSDQHMVRGRTTAPVGQTLGHEITGEVIEKGNDVQFIDEGDICSVPF
                     NIACGRCRNCKERKTGVCLNVNPARAGSAYGYVDMGGWTGGQAEYVMVPFADFNLLKF
                     PDRDQALEKILDLTMLSDIFPTGYHGAYTAQVTTGSTVYVAGAGPVGLAAAYSAQLLG
                     AAVVIVGDLNDDRLAQARSFGCETVNVGGDAELPDQIAEIVGEPEVDCAVDAVGFEAS
                     GRGGEEAPAVVLNSVQSITRAGGSLGIPGLYVTGDPGAADPDAQEGTLKIRFGLGWAK
                     SHAFFTGQCPVMKYNRGLMMSILHDKAHIADAVNATVVTLDEAPEGYKDFDKGAAKKY
                     VLNPNGYINA"
     gene            44548..45114
                     /locus_tag="VN178_11175"
     CDS             44548..45114
                     /locus_tag="VN178_11175"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81576.1"
                     /translation="MLFKLTEKDGLWKKIRNHVGTTVEHPVSPATRDIRSRIFDEGVE
                     KTVSVCPYCAVGCSTLVYHRDGRIIDIEGNPDSPINAGTLCPKGSAAFGLHVSPYRWT
                     KVRYRRPYSAEWEDLSLEEALAMIADRVKNTRDENWQDTDERGKKLSRSTAVASIGGA
                     TIDNEENYLITKLFHSLGFVRITNQARI"
     gene            45127..47664
                     /locus_tag="VN178_11180"
     CDS             45127..47664
                     /locus_tag="VN178_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWS81577.1"
                     /translation="MPGLGITYGRGGATTTLQDLQNSDCILIEGSNMAEAHPVGFRHP
                     MIAKKRGAKLIHVDPRFTRTSALCDIYAPLRPGTDIAFLGGLINYVIQNERYFMDYVV
                     HYTNAATLVTEEFEEAGISGLFSGWDEESRRYDPSSWRYEGTPIDYAAVGGSQGRLIG
                     EGVGGESAPNEDEPRPTDPTLEHERCVFQLLKRHFAKYTPEMVEKVCGTPQEAFLEVA
                     ETICENSTPEKTTSLCYAVGWTQHSKGVQIIRTAAILQLLLGNIGRPGGGIMALRGHA
                     TIQGSTDIATLYNILPGYLPMPDVNMDNDTWSDYVESQTASTGWWNNFPKYITSLFKA
                     WYGEAFDPARGDEVFDHFPRLSGDYSYYPTIVDMKDGDVKGCFVFGQNFAVGGPHVKL
                     AQDALRSLDWLVVLDAYEIETATTWKMEGVNPEECAMEVFFIPSALVAEKDGSFTQTQ
                     RMLQWHDKAVEPPGDARSDAWFVFYLGRRLKELYEDSEEPRDTLIKAMTWDYPTEGER
                     EEPHIESILKEISGYTVADGRPVGGFAELEDDGTTACGGWIYSGVYSGGVNQARRRKP
                     QTDQPPAANEWGWAWPADRRILYNRASADPEGRPWSEEKKYVWWDEQQGEWVGQDIPD
                     FTATKRPDYRPEPDARGDDAIPGDGPFIVNGDGLGWLFAPVGLADGPLPVHYEPVESP
                     VPNLLYDNVQYDPAVRLMDRPDNPYNPPEDPRFPYVVTTYRLTEHHTSGAMSRWIPWL
                     SELQPELFCEISPELAAEKGVNNTGWVTISTSRGRIHCKALVSDRIPVYDFQGKKLHT
                     IGLPYHWGPSGLVKGDVVNDLIPVALEANVAIHEAKAFTANLEAGRV"
     gene            47669..48136
                     /locus_tag="VN178_11185"
     CDS             47669..48136
                     /locus_tag="VN178_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81578.1"
                     /translation="MAARDRALTGVLSGLGATLVLTGLRQAWSKVGLVFETAPMQVVD
                     RVEELGLLGELSPWGRRLLTVAAHFAYGAGTGMSFGLLRREKGGPGEEASVGSALGVL
                     AWGVGWASWLPLTGVHKAPWTQKTPKVLLPVIDHAVFGAAWGLLYWALRKARA"
     gene            48133..48435
                     /locus_tag="VN178_11190"
     CDS             48133..48435
                     /locus_tag="VN178_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81579.1"
                     /translation="MSVGERRSREMMQQDLMDLGVWLSKHPEAAGKNLKPIRSAEALE
                     PGVYYNMGTGMVDRIFTPQRVALGHKMFRISKDPASSVEEVRRKAMEGRGRWRRGS"
     gene            48417..49253
                     /locus_tag="VN178_11195"
     CDS             48417..49253
                     /locus_tag="VN178_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWS81580.1"
                     /translation="MATGFLTDTTVCIGCKACEVACKQWNQLPADGYELTGDSYDNTK
                     QLSGTTWRHVAFIENPKKRRLTGDVDLVALAEDVTEPLPGGAELDALGRWAFMSDVCK
                     HCVEAPCQQACPTGAIIRNEFDNVYIQPDICNGCGYCVPACPYGVITQVPNDGRAFKC
                     TLCYDRQRDGLEPACAKACPTNSIIFGEVEDLKAIAEERVQELHNKGYSQAYVWGTPE
                     VGGTGGIGGNHSKFILMDEPETYNLPRHPYLPQEKVKGGTIASMVAGAVLGAVVALAF
                     RD"
     gene            49264..50301
                     /gene="nrfD"
                     /locus_tag="VN178_11200"
     CDS             49264..50301
                     /gene="nrfD"
                     /locus_tag="VN178_11200"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWS81581.1"
                     /translation="MSDTTQDGVRQSNGKVETSHHPETDPGRGDKNYYGIPPIKHAHW
                     TWQIPVYFWIGGIGAGVHLFSTITQILGHKDEALTRASRYTVLGTMVLSPILLIWDLG
                     RPERFYNMLRILKLRSPMSTQSWSVFAFGNLSGLIAARQAADDGLLGEDNPLVRLVKL
                     IPARALSIVALPVGLNVGSNTGNLISATSVPLWARNWLFMGPTFLTSGLSTALSWLSL
                     VLHLTHSGEKKTLRTLHRAEKATILIEAGLIAASLVRMSRWSKPLFSREVAPLFLGGT
                     LLGGIVAPMVLLFGKESRSRSILASVLALAGGLAFRFAIIKGGRISADDPEAYFTFAS
                     GESAPQPEDSI"
     gene            complement(50310..50708)
                     /locus_tag="VN178_11205"
     CDS             complement(50310..50708)
                     /locus_tag="VN178_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS81582.1"
                     /translation="MKVVTLKDMQTGPGPEDWFTGTVWRDAAPTGTSPDVAVNRVLFE
                     PGARTHWHTHPEGQILYIVTGTCRAASEDEPPVEVEAGGVVYFAPDERHWHGATPETY
                     MVHLAINIATTTDGGTDWMEPVTDQEYAGE"
     gene            complement(50879..52531)
                     /locus_tag="VN178_11210"
     CDS             complement(50879..52531)
                     /locus_tag="VN178_11210"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWS81583.1"
                     /translation="MRPAPGLVLGPLLRYTGETDATVWVETDAACEVEVLGHSASTFE
                     IEGHHYTLVRITDLEPGKTYEYEVFLDGEKVWPDGAHDFPPSVIRTIAPAGVLNLSYG
                     SCRVSVPHKIPYTLASDAHRDGLERDALEALALRMMHEPHERWPDALLLLGDQIYADE
                     VSEGALDFIRSRRDTNEPPGEQVADFEEYTRLYWDSWRDPVIRWLLSTVPTAMIFDDH
                     DVNDDWNISEAWVRQMRATPWWEDRITGAFMSYWVYQHLGNLSPRELEEDALFEKVQK
                     AENPTRVLREFAYKADREIAGTRWSYHRDFGRVRLIMMDSRAGRVLKEGHRSMLDDEE
                     WAWIVEMATGDFDHLLFGTSLPFLLGPGLHHLEAASEAVCAGAWGRLAARIGESVRQL
                     VDLEHWSAFNKSFTELTYLLRSVAAGEHSNGDPPASVTVLSGDVHHGYLAKMDFGDIK
                     TPVYQSTSSPLRNPLGLPERLAMRFGWTERGRRISEALSRLAGLEESDFRWQLLHEAP
                     WFHNHISSLEIRGRKALLKVEKTIPENNDEPHLFPILEHRLA"
     gene            complement(52528..54123)
                     /locus_tag="VN178_11215"
     CDS             complement(52528..54123)
                     /locus_tag="VN178_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HWS81584.1"
                     /translation="MSEDIRPGGTYKTLDEQREVLSPAEERFERRRRTIGLFLGPPVL
                     LIMMLLPLGLPYTQQSLAAVLAFVVVYWVTEAIPIPVTAVIGLALAVILNVPQVATDA
                     EDDPADVIFGSFADDTIFLFIGSFIIAQAMMTHGLDRRFAYLVLSIPGIARSTYGVIV
                     GFGLIAASISGFVSNTATTAMLFPIGLGIMGALAGLVSDQSEGDTDVRRLRFGTALML
                     MIAYGASVGGLLTPIGSPPNLIGREFIEQETGTTISFFEWVVLAFPIVLTMFVLLCII
                     LILLNRPETRQISGAEEFIAEEREKLGGLSRGERNTLIVFGLAVFFWILPGIIGIIFG
                     DESTAYGLVYDRINEGVVAVVAAGLLFILPINWSERRFTLNWNEAVRIDWGTILLFGS
                     GIALGTLLSETGLAETIGTGLTEGLGVSSLFAITLLSTIVALIISETTSNTASATIVV
                     PIVLPIAASAGVNPVIPALAAVFGASYGFMLPVSTPPNAIVYGSGMIPITKMIRSGIV
                     FDIIGVVLITFGITVMARLVGLA"
     gene            complement(54197..55291)
                     /locus_tag="VN178_11220"
     CDS             complement(54197..55291)
                     /locus_tag="VN178_11220"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWS81585.1"
                     /translation="MDGGFGATAPYTVGVEEEFQLVDPASRGLTPAVEEVLAAGDGSR
                     LITSELSRSCVEMLSPIFASAADLARELPGLRREVREIARSSGVEIVAAGAHPFSEPT
                     EQPLTGGDHNRRVEEEMGWVARTQAIYGLHVHVAVPDGDAAILAVNTLARYVPLLVAL
                     SANSPFWRGFDTKLATTRIKIFEMFPRSGLPPTFRNWEDFEDHIEALVASGSIPDYSW
                     CWWDARPHQKFGTVELRAPDVQTDPSYTVALAALVQCLVARAGVEKRPAEDAMYNAEN
                     KWRAVRYGMDADFYDFVAGRAMPARSMARGLVEELRPVARDLGCEEELCGVLEIVELG
                     TGAELQRAAHKRSGSLEGVVDYLIEGTVRG"
     gene            complement(55486..>55941)
                     /locus_tag="VN178_11225"
     CDS             complement(55486..>55941)
                     /locus_tag="VN178_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81586.1"
                     /translation="LLALRSRYERLPGAKLRERLGRTFGFHRDDGFSLGPLSVLPGTF
                     EMGALRVEWETQLILRSSEPMPGISWWTEGRHNLLPRRTDEARKLIFALRYTGDSVRE
                     LKRRYGYKTERWHATVPEGKLFDAVRDLESSHRIQLALSRSGFRKAQPS"
BASE COUNT        10246 a        17677 c        18324 g         9694 t
ORIGIN      
        1 aagacacgaa aaggagaaac ggtcgtcgag gccgacgagg ggcccaggcg cgacacctca
       61 gtggagaagc tgggcaagct caagccagcc ttcaagggcg atggtagcgt cacgcccggc
      121 aactccagct ccctgaacga cggggccgcg gcggtgctgg tggtttccag ggagtacgcc
      181 gaggcgcacg gcctcgaacc cctggcaaag gtacggagca tcgccgtcgc cggggtcccg
      241 ccccgcgtga tgggcatagg cccggtcccg gcgacgaaga aggccttgat gagggcgggg
      301 atctcgctag aggaggtgag cctcatcgag ctcaacgagg cgttcgcggc gcagtcgctg
      361 gcggtgctcc acgaatggag catggacccg gaggacgaga ggctcaatcc caacggcggc
      421 gcgatagccc tcggtcaccc cctgggggcg tcgggggcgc gcatcctgac tacgctcctg
      481 cacgagatgc ggcgccgaga cggcgtccgc tacgggctgg cgacgatgtg cataggggtc
      541 gggcagggaa tcgccatggt cgtcgagaag ccggcctgag ggagaagcga cggtgagaca
      601 gcaacccacc gacgaaaccg gcggtttacg cccggaggat aacgggcagc cgccgtacct
      661 gtatccagac tacgtggcca cgcgcctgag ggcgccgaag gaacccctga tcatactccc
      721 caagaccctc tccgatacca cgggtccggc ctacgggcgc ggccctatcg gggagctgga
      781 caacgacctg acccgtcagc acgggggcga acctctggga gagaggataa tcgtcgcggg
      841 acgtgtgttc gatggtgatg gacgccccgt gcgcaaaagc ctcgtggaga tctggcaggc
      901 aaacgccgca ggacgctaca cacaccatct ggaccagcac cctgctccgc tggacccgaa
      961 cttctcgggg gcggggagat gcctcaccga cgacgagggc cgctacaggt tcgtaacggt
     1021 aaagcctggg gcttatccct ggaagaatca cgacaacgcc tggcgaccgg cgcacataca
     1081 cttctcgctc ttcggtccgg cgttcaggag caggctcata acccagatgt acttccccgg
     1141 cgacccgctc ttcgagcaag atcctatatt tcgatcagtg cgggacagag aggctagaca
     1201 acgcatgatc tcgaccttcg acctcgaaac cacgcagccc gaatgggccc ttggataccg
     1261 cttcgacatc gtgctggaag gaaggaccgc caccccgatg gagaacccca ggtgagcccc
     1321 gagccaacac cttcccagac gattggaccg ttcttccacg atgcgctact agatgagaag
     1381 tactccgagc tcgtctcgct cgaccaccct gaggctatag tgataagagg aacggtctat
     1441 gacggggctg gcgaggccgt gcctgacgct atggtcgaga tctggcaggc caatagggag
     1501 ggccgctacg atcgttccac cgacggcagg catgatccgc cccccgacga agatttctcg
     1561 ggtttcggac gctcgggcac ggacgcaggc ggcgtgttct ccttcttgac ggtcaagcct
     1621 ggacccgtcc ccggcccgga cggccagctt caggcaccgc acgttatggt ctccgtcttc
     1681 gcccgcggcc tcttgaagcg tttggtgacc cgcatctact tccccgacga ggaagaggcc
     1741 aacgccgtcg accccgtcct ctcgtctgtc gaagatcagg gacttcggag gaccctcata
     1801 gcccgtgacg aaggggatgc tctgcgtttc gacatccacc tgcagggtga tgggcagacc
     1861 gcgttcttcg cgttctagag atggctggag agctgttcag gccgatcttc gtccccgaga
     1921 ggttttcgga agccgtctcc gggagggcct ggatccgggc gatgctcgaa gctgagggcg
     1981 ctcttgctct tgcgcaggcg cgggccggtc tgatcccccg ccaggcagcg gagaccatcg
     2041 tctcctgctg cgacgctgag agctttgttc ccgaggagat aggacgcgaa ggacgcgccc
     2101 agggcaaccc cgtcccgccg ctcgtccggg ctctcaccag ggcggtctca gaagtctccg
     2161 aagacgccgc cttccacgtc cacaagggtg ccacgagcca ggacatcatg gacacggctg
     2221 cgatgctggt ctctcgacac gctctcggcc tgatcctcgc cgagatagac ggaatctcgg
     2281 cggcctgcgc acgtctcgcc gatacgtata gagacaccat gatgcccggg cggacgctgc
     2341 tccagcaggc gctcccgacg accttcggca agaaggccgc cggctggctg gtctcggtgc
     2401 tcgaggctgg aacgcggctg cgtgagatgc gccagtctgg gctcgccgtg cagttgggtg
     2461 gggcggctgg gacgctggcc tcgctcggtc cggcgggcac gaaggtcctc ggcgagctcg
     2521 cgcgggagct cgaactcgcc gagcccgtaa ttgggtggca cacagacagg gtcaggatct
     2581 cggagatcgg aagcgccctc tcgctcgtcg ccggcgtact cggcaagatc tcacaggacg
     2641 ttatcctgat ggcccagacg gagatcggag aggtcgccga acctgcggga gagggaaggg
     2701 gaggctcttc gacgctcccc cacaagcgaa accccatact ctccgtgatc gccgccgcca
     2761 acgtcagaag ggtactcgac ctctcgcaca cgctgcaggc tgctatggtg ggcgagcacg
     2821 agcgggccgc gggcgcctgg cactcggaat gggaagcact ctcggacgcc ctcgccctca
     2881 cgggcggggc cgccgcagcg gtcagggagg tgaccgaagg attggaaatc tatccggaga
     2941 agatgctgga gaacctggac gccaccggag gcctgctgct cgcggagaac gtcgtcaccg
     3001 tcgcggcgca gcatctcggg cgtttagaag cgcacgacct catcgaggaa gcctcgcacc
     3061 gagccctcga aaacggaaag cctctacgcg aggagctact atccggactc ggcgagattc
     3121 tctccgagga ggagatagat gcggccttgg atccggcaca ctacctcggg tctgcagggg
     3181 agttcgtgga ccgggctctg aaacgatatc gtgaggggga gatcaaatga acgaaggtac
     3241 catagtggag ttgaatcaca tcctcgaagg accggaggac gcacctgtcc tcgtcctgtc
     3301 caactctctc ggctccacgc tcgggatgtg ggacgaccag gcgcctattc tccgcgagcg
     3361 tttccgcctt gtgcgctacg accaacgcgg gcatggtggt tcgcccgttc cgtccggtcc
     3421 ctacaagata gaagacctcg ggcgggacgc gctggcgctg ttggacaggt tggagatcga
     3481 aagcgcttcg ttctgcggcc tctccatcgg cgggctcgtc ggcatgtggc tggcgagcga
     3541 agcgcccgag cgcgtcgagc gcctggtggt gtgttgcaca gcagcccgat tcgaacccga
     3601 gatgtacgac gcccgcgcca gcaaggtcag ggccgagggt gtcgattcta tcgccgacgt
     3661 ggtgctagag cggtggttca cgcccgagtt ccgcgccgcc cgacccgaga ccgtggagtc
     3721 ggcagggagc atgttgcgta gcacgccggc cgaagggtac gcgggttgtt gcgaggctct
     3781 cagggacgcc gacgtgcggg acaggcttgg ggagatccgg gctcccacgc tcgtgatagc
     3841 gggcgcggaa gaccccgcgg cgaccgtggg tcaggcagag gagctctgcg actcgatccc
     3901 ggaggcacgc ctcgtcgtgg tcggggccgc gcacctggcc aacatcgagc agcccgaagc
     3961 cgtgacgcgg gagatcctgg aacatctcga gccggccttg ggggacggga gatgacaccg
     4021 gaagactcta cctacgatcg cggtatgaaa gtccgtcgcg aggtcctcgg cgacgagtac
     4081 gtggaccgga ccatcgggcg cacgacggag ttcaccgcgg acttccagga cttcataacc
     4141 aggtacgcct ggggagagat ctggtcccgc cccggcctgg accgcaaaac acgaagctgc
     4201 atcacgctca ccgcgctagt cgcgctgggc cgcctcgacg agctggagct gcacgtgcgc
     4261 gccgccgtcc gaaacggcct gacggaacag gagataggag aggtcttcct gcagtgcgcc
     4321 gtctactgcg gggcgcccgc ggccaactcg gccttcgccg tggcccaacg cgtcttctcc
     4381 gaaaccggga agggagagta ggtgcgtacg caggtaggga tcgtcggggg tgggcccgcg
     4441 gggctgttgc tctcgcacct cctgcacctg cagggaatcg aatccgtcgt gctcgaacgc
     4501 cgcagccgcg aagatctgga gacgacggtc agggcaggcg tgctcgaata cggcacggcc
     4561 gggttgctga gtgaggtcgg tgtcggagag aggatggagc aggaaggttc cgtccaccac
     4621 gggatcgaac tccgcttcga tggtcgcggg caccgcatcg acttcgccga cctgacggac
     4681 gggctttcga tcacgatcta cggccagcag gaagtcgtaa aggacctgat ccgtgccagg
     4741 ctcgaagcgg acggaaggat cctgttcgag gccggcgccg aaagcgtaga agccccggac
     4801 actgactcgc cgaagattcg attctggcag gatggtgagc aagaggagat ctcttgcgac
     4861 tacgtcgccg gctgcgacgg ctctttcggg gtttgcagga gcgggatccc tgaggacgca
     4921 cgcacggagt acaccaggac ctatcccttt gggtggttcg gtatcctcgt cgagggaccg
     4981 ccatcgacac aggagttgat ctacaccctc cacgaccgcg gcttcgccct ggtcagcacc
     5041 cgctcccccg aggtccagcg cctctacttc cagtgcgacc cgcaagacga cgtcgaagag
     5101 tggcctgacg agaggatctg ggacgagctc tccacgaggc tagccaccca cgatggctgg
     5161 gagctggccg gaggcaggat cttccagaag gacatcgtgc agatgcgttc cttcgtctgc
     5221 gagccgatgc agcacggaag gctcttcctg gccggggacg ccgcgcacat cgtcccgccc
     5281 acgggagcga agggtatgaa cctggccgtc gcggatatcc gggtcctcgc ccacgccctg
     5341 acggagttct acagatccgg agattggcga ctcctcgatg cgtactccga gacgtgcctg
     5401 cgccgcgtct ggagggcgca gcggttctcc tggtggatga cctccatgct gcaccgcttc
     5461 gacaggagcg atgctttcca gctcaaggta cagcaggccg agctagacta catcacgacc
     5521 tcgcgggccg cggcgacgac catcgccgag aactacgtcg gagcggctct gggcacctcc
     5581 caccctctat aatgggcggc gtgtcgagag ggaggatcag cggcaagacc agactgcttg
     5641 ccctcatcgg gcaccccgtc ggccacagcc tgagcccggc gatgcacaat gcggccttcg
     5701 ccgcggatgg cctcgacttc gtctacgtct gcctggatgt ggatccccac gacctaccgg
     5761 cggcggtgag gggcctggcg gcctcaaagc tcaggggttt caacgtcacg atgccgcaca
     5821 agcgggccat gatcccacta atgaacgagc tggacgagag cgcacgcgtc tctggcgccg
     5881 tcaacaccgt ggtgatcgac gcctccgaaa tgcgcggttt caacacggac gggggcggga
     5941 tggtgatggc ctgccaggag gccggaatag aactctctgg caggaacatc ctgctcctcg
     6001 gcgccggggg taccgccgcc gccatcgcgg tcgccttcgg taaagccgga gccggtgaac
     6061 ttcacatcgc caaccggagc gtggagcgcg ccgcgcacct gcgagacaag ctgtacagga
     6121 cgggaatgaa aggactcgcg gtccaccacc taaacgcgct ccccgatgcg gagatcgtca
     6181 tcaacaccac ccctctgggg atgaaggagg gggaccccat cccggtgccg cccgattacg
     6241 tacaggaagg cagggcgttc tgcgacgcgg tctaccgtcc agggaccgag actccgctcg
     6301 tccggctggc ccgcgagcgg gggatccccg tcgtcgccgg cggcaggatg ttgctctacc
     6361 agggcgtact ggcgcagaag ctctggaccg ggcgcgagcc gaacgtgaag gctatggacc
     6421 gcgcgatttc ctgatacttg aggcgcggag tgcctgccac ggtggattcc cgctttcgcg
     6481 ggaatgacgt atgagagggc tcggcgcgcc cacatcccga acgtggtcac gctcaaccgg
     6541 actcgcttcg caagctgaaa tgctgaagtg ctgaaatgct tcaaaacgga taaacgacta
     6601 ctccgagcgc cccgagcgcg taccacgctc cgaaggccat gctaaggacg cctagcacgg
     6661 gggcgacacg ctcgaaattg cgcgctatgg gaccgccggc tatgatgagg ccgaaggccg
     6721 tggagaggag cgccatcgag acggcggtcc cggcggcgaa gaggatcagg gcccccgtcg
     6781 cctgagcttt ttccgagatg gtcgagagca gcagcagcgt gagcccgcca gaaccgccta
     6841 tgccgtgcac cagcccgatg ccgtaggatg aaagcggggt gtgccgaggg acgcggtgga
     6901 cgtgcccgtg ggactcgtcg tgggcgtggc agtggacgtg gcggtgggcc tcaccgccgt
     6961 cgtgggtgtg gacgtggaca tgataaaagc cacggcgcca ccgaatgaga agcctcactg
     7021 ccagcagcac aatgatgcat ccgatggcga cttcggcaac cttgctgatt acctcgggca
     7081 ggtactggtt caggaggacg agcggcaggc cgatcagtac gagcgtggtc ccgtggccca
     7141 ggccccagag gaagcccata gcgccagcct tccttacctg gtctcgctcc ttctcggagg
     7201 cgatgagggt tgtgacggcg gcgaggtgat caggatcgct cgcgtgcctg agaccgagca
     7261 gcaggctgac gagcaggatg acccctacgc cgacctgtcc gtgcaagaac ccctcgagcc
     7321 agccatctat cacgcccaca cgttcctcct agccttcgtc ttccaacgcg gccctcacca
     7381 actcgcgtcg tacttcctgc ggtagaagcg ctggtccacg aacgcctgca cccgactacg
     7441 cagcgggttg aacaacgccg ctatggtcag cgtggaggca acgaccccga gcgtggactc
     7501 ctgaccgcca agtacgccca tgacggcctg cagaccgacc acgccgccga cgtagtccgt
     7561 caccagcata gcggtaagcg aatctttcca actacccttg tgttctgcat ccctgttcct
     7621 gctgtcacag caccactcta caagatttct gtctcccgca cgctacgtaa gcgttgatcg
     7681 tgtctccgag tatcgcacca aggcggagtt cacgcccgaa gttctcggta cccaaacaag
     7741 atcggtttcg acacaccgcc ggcgagtccc cataatgagt taacatgccc cgtcaaagcg
     7801 aaggagcgag ggcggatgga agagtccgaa caaaagatcg tggaaagtaa ggagatcagg
     7861 gcacgctacc cccacttcct gcgtgtaccg acgcgctgga tggacaacga cgtctacggg
     7921 cacgtcaaca acgttatcta ttactcgtac ttcgatacgg tcgtgaacga gtatttgatc
     7981 cgctcgggcg tcctggacat cgagaggagt ccggttatcg ggctcgtcgt cgagacacag
     8041 tgcctgttct ttgagcccct gaccttcccc gacaccgtcc acgctggtct gcgggtggca
     8101 cgtctgggac gcagcagcgt gcgctacgag atcgggctat tcagaaacga cggcgagatc
     8161 gccgctgctc agggacattt cgtccacgtt tacgtcgagc gcgtgtctcg caaggcgacc
     8221 ccccttccgc cggggatgcg agcggcactc gaaaagatcc tcgccagcca gtagcggcta
     8281 gttcgattcg tatgagccga ccagcgaggc gacgtgtccc gagatcaggg cccccatctc
     8341 ctcgaggagg gcttcgtctt cctgtgagaa ggctccgacg gtatccgaat cgatatcgaa
     8401 ctcgccgact atcctgccgg cgtcccggat gaggacgacc agctcggagc gggtgtagat
     8461 cgagcaggcc aggtagttct cgacctcgcg cacgtcctcg actatctggt tgcggccctc
     8521 ggagaccgct gtgccgcaga cgccctcccc tatcccgatg cgcgagtggt ccaccggcgt
     8581 cgtgcgggga ccgagtatga gggtgtcccc cacaagcaga tagatgccgc tccagtccca
     8641 cgcaggatgc gcctcatgca ccttccgcac gacctcgttc aatacctcct cgtcggacgc
     8701 atccgaggcc acaagcgcct ctacctcgga tctcaaatcc tcgcggagcc ccgtcttctc
     8761 actcatcgat ggaatccttc cgtatccgga acgtctccca tcaataataa cgccgcccag
     8821 gaaagaaatc cgtgaagatc ctacgaagcg acctgatgcc gcaggttgaa gggttgatct
     8881 tccaaaaacc gctctagcct gagaagaatc ccgatgtgtc ccacaccagc aggacgccgc
     8941 cgctcgtggg gtcttgcgtc acgctgatca gcttccagct ctggcgtgcc ccctcgttga
     9001 tcgcctcctg caaggcagcc tccgaggaac cggactcgcc ggcctccagc gaagcgtgct
     9061 gcgcgtagta ccgctcggcc tccggacggt cgggagcctc cctctgtccc tcagtcatgt
     9121 cctacctcct tcatttctgt cgcccctcgg gcaacctttt ccacacaaac atgatgccac
     9181 caagcggagc cagtcgcacc cgccagaggt ccagcagacg attgccgagc cgttactaca
     9241 atattacagt tctcaatcat ggcgacgtaa gataggtaag tggaaagcac cctcaaaatg
     9301 cacgaaaaag gccggcgtgc tagactgcgc gcacttccgc ctagaaaggg gaaaagggag
     9361 caaaaccgcc ggatgtaaag gaaaaacgcg gcggatgtaa atacgttgac agcggcctgt
     9421 ggagggccgg tagaacgtgg atttcgggtg ggtcgtacag gcgctgagtc cattttgggg
     9481 aaaggagaga aggttgcgca ggatccagat gacggtagac gggaacgccc acgtggctga
     9541 ggtggaaccg aggttgctgt tgctacacca cctcagggag aacctcggga tcgggggcgt
     9601 gcataccggg tgcaacacga ccagttgcgg ggcctgcacg gtccacatcg acggtgagtc
     9661 ggtcaagtcg tgtacggtcc ttgcggtgca ggccgacggg cacgagatca ccaccgtcag
     9721 gggcatcacg gagaacggtg tgtggcatcc catgcagcag gctttccacg aggcgcacgg
     9781 tctgcagtgc ggctactgta cgccgggcat gatcatggcc tccatcagcc tcctgaagga
     9841 gaatccggac gcgacagagc aggagatcag ggaaggcctg gaagggaacc tgtgccgctg
     9901 cacgggctac gagaacatcg tgaaggccgt ccagctcgtc cagcagcacg aggacgagat
     9961 ggcccagcac gcggcatcct agaggaggaa agagagtgac ccaagcaccg gagaagttcg
    10021 tcggaggcgg cgtactgcgc aaggaagacc ctgccctcgt aaccgggcgg gccacctgga
    10081 cggacaacat cacgctgccg gggatgctac atgcggtgtt catgcgcagt ccctacgcgc
    10141 acgcgaagat cacgtcgatc gacgtctcgg ccgccgcaga gcagcccggc gtcgtcgccg
    10201 tctttacggg cgaagacctt atagacgagt acgagaagca tcccagggag aaccccgcgg
    10261 ccgtcaccgg ctacgacctg agcctccacg atcgcaggca gggccacacc gtcacgggcg
    10321 agacagaagg aggggacggc cagggcgccg acgagccgcg ctcgttcgtc gcctggatcg
    10381 tccacgacga gctcaagatc ccgcaccacg acccgctggc ccacgacgag gtcaacttcg
    10441 cgggcgaacc cgtggccgtc gtggtcgcga ccgaccgcta caaggcccag gacgccgtcg
    10501 agttcatcga ggtcgactac gagccgttgg acgtggtgac ggacctggaa gaggccatga
    10561 ccgaaggctc gccgctggcc cacgagaact tcgggacgaa cgaggcttat acctgggacc
    10621 tcgcgacggg tgacatcgac gaggccttct ccaaggcgga tgtcaccgtg aagggccgct
    10681 acctgcagcc gcgtctgatc cccaacgcca tcgagacgcg gggcgtggtc gcgagccccg
    10741 acccggtaag cggcgggttc accgtgtaca cctcgacgca ggtgccgcac atcaccaagg
    10801 ccgtgctctc gtcgtacctc ggggtgccgg agcagaagct ccgcctggtc gcccccgacg
    10861 tgggcggggg cttcggctcc aagctcaacg tctacgcaga agaggtcatc gcgctcgtcc
    10921 tggcgcgaag gatgggcgtg ccgatcaagt ggatcgaaga ccgctccgag aattacctgg
    10981 ccaccatcca cggccgcggg caggtacagg acatcgaggt ggccgccgac tctgagggca
    11041 agatcctcgg catgaaggtc aacctgctcg ctgacatggg cgcgtatctg cacctgaaca
    11101 cggccggtat accgttgctc ggcgcgttca tgtaccccgg cctctacacc ttccccgcct
    11161 actacttcga gtgcatcggc gtctacacca acaagacccc gaccgatgct tatcgtgggg
    11221 ccggtaggcc agaggccgcc ttcgccgtcg agcgcatcat ggaccagctc gccgccgagg
    11281 tagggatcga tcccgccgag gtgcgccgcc gcaacttcta cgagcccttc gacgagccga
    11341 ccgatacccc cgccggcatc cagtacgact ccggcaacta ccagatgacg ctcgatcggg
    11401 taatcgagct tgccgactac gaggggctgc gtgaggaaca acgccggagg cgcgagtcgg
    11461 gcgaccctgt acagctcggg atcgggttct caacctacac cgagatctgc ggccttgccc
    11521 cctcgcaggt gctcacggcc ctcggggccg gaggggccgg ctgggagatg gcgaaggtcc
    11581 agatgctcgc ctcgggtaag gtcgaggtca ttacaggaac tacgccccac ggacagggcc
    11641 acgtgacgag ctggtcccag atcgccgctg acgtccttgg tgtcacgccg gatgacgtcg
    11701 aggtcctaca cggcgacacg gcaatagccc cctacggacg cgacacttac ggctctcgca
    11761 gcctccctgt cggcggcgtg gcggtacata tggcggccca gaaggtcgtc gagaaggcca
    11821 ggaagatagc ggcccacatg ctggaggccg ccgaggacga tatagagttc gagggcggta
    11881 ctttcagcgt cgccggctcg cccgaccaga acgtcaccat ggccgatgtg tccggggccg
    11941 catacctggc gaacaatctg ccagagggta tgcagccggt actctccgag gagttcttct
    12001 tcgacccgcc caacttcacc ttcccgttcg gggcgcacat ctgcgtcacc gaggtggata
    12061 cggagaccgg gttcgtgaag atcagggact acttcggcgt cgacgactgc gggcccgtca
    12121 tcaatccgac catcgtcgac ggacagctcc acggtgggat agcgcagggc gtagcgcaag
    12181 ccctctacga ggaggcggtc tacgacgagg agggcaacct gacgacgggc tcgatggtcg
    12241 actacctgat ccctggcgca ccggagctac cgaattacac cctggagcgg acggtcacgc
    12301 cgtccacctc gaacgagatg ggcgtgaagg gcgtcggcga ggcggggact attggggcgc
    12361 ctcccgcggt catcaactct atcgttgatg cgctctcgcc ctacggcata acgcacgtag
    12421 acatgccggc ctcgccgtgg aaggtgtggc aggccatcca ggacgcgcag gggaaggaag
    12481 caggcacccg cgacgcagac cgctccggac gcccctccca ggcggtcgac agcaccgaca
    12541 cggaaggagg tagcgtctcg tgatccccct cgccttcgac tacgaggtcg ccgaatccgt
    12601 cgaccacgcc atcgagctgc tcgggcaaca cggcgaggag gccaagctcc tggccggcgg
    12661 ccacagcctg ctccccataa tgaagctcag gctcgccgcc ccgacggtcc tcgtcgatct
    12721 cggtcgcata gacgacctga actacgtccg cgacgagggc gatcacctcg ccatcggcgc
    12781 gatgacccgc cacaccgaca tggaacgcga cgccaccctg aaggagcact gcggcctcat
    12841 cgcctacacg gcctcgctcg tcggcgaccc gcaggtcagg caccgcggca ccatcggcgg
    12901 ctccataagc cacggcgacg cggcatcgga cctgccttcg gcgctactcg ccctcgacgc
    12961 aaccttcgtc gtaaagggcc ctggcggcga gcggagcgtg gccgcgggag acttcttcga
    13021 agattacctg cagaccacgc tcgcccccga cgaggtgctc acggagataa gggttccgaa
    13081 actcggcgga gcgggctggt cttacaagaa gttcaaccgt cgcgcccagg actgggccgt
    13141 cgtcggggtg gccgccgtcg tggagcggtc gaacggttcg atcggctcgg cccgcatcgg
    13201 gcttaccaac atgggttcga cgcccttgag ggcgaccgcg gcagagaacg cgctctccgg
    13261 cgcggacccg tcttcggtcg ccgaggctac cagctctgcc gacgaaggca cgagcccttc
    13321 atcggacatc gcggcctccg acgagtacag gcgccacctg gcgcgcgtgc tcagcagacg
    13381 ggccgtcgag gaggcactag agcgttgacc gaagcttctg acggacgccg gggggccttg
    13441 ccccccggcg tttctcattt ccgggagctg ggagcgcggc tgcgcgagca cgattatctg
    13501 gctgacgaag ggctgtccac cgcgatcttc ctctccctca agctcggaag gccgctgttc
    13561 ctcgaaggcg aggcgggggt cggcaagacc gaggtcgcca aagtcctctc ccgcatccta
    13621 aagacgcctc tcatccgcct gcagtgctac gagggcatag acgtctacca ggcagtctac
    13681 gaatgggatt acgcgcgcca gctcctccac atccgcgccg ccgaagcctt tgggggcgaa
    13741 gaccgggaac gcgtcgagcg cgacctctac gaccgcgagt tccttaccag tcgtccgctg
    13801 ctccaggcgc tcgagcatcg cgggccgatg ccgcccgtgt tgctcataga cgaggtggac
    13861 cgctccgacg acgagttcga ggcgttcttg ctggagatcc tctccgactt cgccgtcacg
    13921 atccccgagc taggcacggt catggccgaa cgtccccccg tggccatcct gacctccaac
    13981 cgcaccaggg aggtacacga cgcgctcaag cgccgctgcc tctaccacta catagagcac
    14041 cccagcttcg agcgggaggt cgagatcgta cgcctgaggg tccccgacgc cgaggagagg
    14101 ctggcccgcc aggtcgccgc tgcgacggag aagatgcgca cgatggacct ctacaaacca
    14161 cccggcgtgg ccgagaccct cgactggacc gaggcgctcg tcgccctcgg cgcgaaggag
    14221 ctcgacgagc aactcgtcga ggcgaccctg ggctccgtcc tcaaataccg ggaggatcag
    14281 cagcggtccg ccaactcggg cctcgccgcg ctcgtcagcg gcgtgggcgg agcagggaga
    14341 gatgcctgag ggggagatcc aggccccgcc gatgctcgac gcggctgtcg ccttcgggcg
    14401 ggtgctgagg ggggcaggcc tgcgcgtcgg aaccgaccgt ctcgtggagt tcgcccgcgc
    14461 cgtcgaggag atggacccct cccgccgcga cgacgtgtac tggtcgggca ggatcacgct
    14521 gacctcccgc cccgaggata tagagatcta cgaccgcgcc ttcgagctct tctgggagac
    14581 cgggggcggc gccaagccac ggccacgccc gaaggtacgg ctctccgtgc cgcaacccga
    14641 ccgctccgtc atgcccccca agaagaccgt ggagaagagc gagtcaggcg aagaggccgt
    14701 caggctccgc tacagcccgg tcgaggtgct gcgtcgcaag gacttcgccc tcttctcgcc
    14761 cgaggagttc gccgagctcc aacgcctgct ggcagacttg aggctctccg gggcgctcag
    14821 aaagagccgg cgcctggaac ccgctccgcg cggacgccac gacccccgcc gcacgcttcg
    14881 gggagccatg cggaccggtg gtgaggccgt gaggcaccgt ttccgcaagg cgagggtgca
    14941 gccgcggcgc gtcgtattgc tctgcgacgt ctccggctcc atggcccctt acagccgcgc
    15001 gcttttgcgc ttcctgcacg cgagcgtcgt cgcggggggg cggctggagg cgttctccct
    15061 gggaacgcgg ctcaccagga tcacccgcga gctaacgacc cgcgacccgg acagggcgct
    15121 caggcagacg gcaggcgcgg taaaggacat ctccggcgga acgcggctgg gcgatgcgat
    15181 caaggagttc gtcgaccgct ggggacagcg gggcatggcg cgtggggccg tcgtcgtgat
    15241 cttctccgac ggctgggaca ggggagacgt atccgtgctg gccgaacaga tgcagcgcct
    15301 gagtcgcctc gcctacaggg tcgtctgggt caaccccctc aaggccgccc ccggctacaa
    15361 accgctggcg gcagggatgg ccgctgcgct cccgcacgtg gacgtcttcc tctcaggcca
    15421 caacttcgag tccctggaag agctcgccca cgccgtcgcc gccgcaacag agcgtgagag
    15481 ataaaatgaa gctagcactt cagcatttca gcacgcctgc ttcgcaggct tgtcagctag
    15541 tcagcatgtc agctacgtgg agtgtggtga gacgctcgtg cgactgcgag gatcccagag
    15601 gagcaccgac tcatcagaac tcgtctccaa ctgtgaaacc tgaaatgctg acgagcggag
    15661 gcgaagccgg agcgtgctga aatgctgccg actgaaagga gacaccattg aatcccgtca
    15721 ttgacaaagt cgcacagtgg agcgaagaag gaaagcaagc ggccctcgcc acggtcgtaa
    15781 aggtcgagcg ttctgggccg ctggggcccg ggacctcgat ggccgtctcc gaggacgggc
    15841 aggtggtcgg gtccgtgagc gggggctgcg tggagcctgc ggtctacgag gaggcgatgt
    15901 ccgccataga gggcggcgag cccaggctcc tctcctacgg catctccgat gacgaggcgt
    15961 tcggcgtcgg gttgacgtgc ggtgggacca tccacatctt cgtcgagcgc gtggactggt
    16021 aatggcggat ctactctccc gctggcacgc ggcactctcc gagcagtcgc ccgtggccgt
    16081 ggcgacggtt atacaggggc cggaggggta cctcgggggt aagatcctgg tctccccgga
    16141 agatcacacc gggactgcgg gcaacgtcga gttggacaag gccgttgtgg aagcggcgcg
    16201 aggtttgctg gagggtgggc gcacggagac ggtccacctc ggtccgcgcg gccaacgcag
    16261 gatggaggac gtcgcagtct tcatccagtc tttcgccccg ccgccgagga tgtacgtctt
    16321 cggcgccata gacttcgctt cggccgtcgc gagggccggc aagttcatgg gctaccgggt
    16381 gatcgtctgc gacgcccgcg ccgtcttcgc caccagggag cgcttcccct cagcggacga
    16441 gatcgtcgtc gcctggcccg atgagtacct gaagaccgcc gaggtcgacg gccgaagcgt
    16501 catcgccgta ctgacccacg accccaagtt cgacgtgccc gtactcaagg aggccttgag
    16561 tacgccggcg ggctacatcg gggcgatggg gagccgcagg acgcacgaca accggacggc
    16621 ccgcctgaag gaggagggcg taacggacga gcaactcgcg cgcatcagca gccccatcgg
    16681 cctggatatc ggggcccgca cccccgagga gacggccata gcgatcgcgg ccgagatcgt
    16741 cgcgctgagg accgggcact ccggcggacg cctcgccgaa agatccggcc ccatccactc
    16801 ggccgcgcca gacaccagga ccagggacgg agtaaaatag tccgccaagg ggtacgtgat
    16861 ccggtcctcc ggccggatga gaggggaact ccaacgtcgt tggagagagg agaggcgcgt
    16921 gcaggttccg gcacgtttcg attacgaggt cgctggcagc gtgggggaag cggtccagct
    16981 tctggagcgg tacggtgagg gggcgcgcct gatcgccggt ggccacagcc tcatccctat
    17041 gatgaagttg cggctagccg cacccgaggt cgtcatagac atcagcgggt tggagagcga
    17101 gctgcgctac atcgaggatg gggatggagc acttaggatc ggggcgctca ccaggcaccg
    17161 cgaggtgctc gattctcctg tcgtaaggga gtggtacgcg ctcctggccg acgcggagag
    17221 gatgatcgcc gaccccctcg tcaggaacat ggggacgatc gggggagacc tcgccaatgc
    17281 ggacccggcg gaggatctcc cggcggcctt cgtcgccctc gacagcgagg tcctggtccg
    17341 aggtcccgac ggtgggcgta ccttgcacat cgacgatctg tacgtcggcc cctacgagac
    17401 ctccctcgat cacggcgagg tcctcacgga gatccgggtg ccgcgcgcac cgggagggag
    17461 cgcctactcc aaggtcaagc ggaagacagg agactacgcc gccgccgcga tcggtgtcgc
    17521 cctgagcatg gagggcgagg aaatagagag catccgtatc ggactgtgcg gcgtcggaag
    17581 ccgaaccctg cgggcgaggg gtgcggaaga agcgctcaca ggggaacgcc ccgggtccga
    17641 agcgtacagg agggcggggg agatcgccgc cggggagtgc gatcccatag atgatgccag
    17701 gggctccgtg gagtacaaac gcgacctggt cagggtactc ctgggacgta cggtcgacaa
    17761 ggcggtcgag cgcgcggccg ggcgggaggt ttagaggtga ggataaacgt caacgtaaac
    17821 ggcgcaatgc tggaggccga ggtcgagccg cgcctcttgc tcgtccactt cataagggag
    17881 accctggggc ttacggggac ccactggggg tgcgacacct ccaactgtgg ggcctgcacg
    17941 gtgctcctcg acggagaacc cgtcaagagc tgcacggtgc tcgccgtcag ggccgactcc
    18001 cacgaggtga cgaccatcga ggggttgtgg agcgggacgg atctcacccc cgtacaggag
    18061 ggctttcacg tcgagcacgg tctgcagtgc ggcttctgta cgcctgggat gatcctgacc
    18121 gcaactgagt tcctggagcg caatcccgac ccgacggagg cggagataag ggaggccatc
    18181 tcgggcaacc tctgccgttg cacgggttac gagaacatcg tcaaggcggt ccagtgggcc
    18241 gcgagaaacg gcagtgcaca ggaggtcggc gcgtaatggc tacggtgcag ggacataccc
    18301 tcggcgaccg gatgcctcgc aaagaggacg ggcgctttat caggggcaag ggaaactacg
    18361 tcgacgacgt aaggctgaca gggatgctac attcggccct cctgcgcagc ccctacgccc
    18421 acgccaggat caatagcgta gacacgagcg ccgcgctcga gctctccggc gtccacgcgg
    18481 tcataacggg cgatgacctc gccgagcagg gcctggcctg gatgcccacc ctcgccggag
    18541 atacccaggc cgtgctcgcc accgacaagg tgcgcttcca ggggcaggag gtcgccttcg
    18601 tggtggccga tagcacctac atcgcgcagg acgccctcga gctcatcgac gtcgactacg
    18661 agccactcga ccccgttata gattccagga aggcgctgga tgatggcgtg cccgtcatcc
    18721 gcgacgacaa ggaaggcaag gacgacaact ccatcttctt ctgggatgcg ggggacaagg
    18781 aggcgactgg caaggcgttc gaggaggccg aggtaaaggt ccgccaggag atgtactacc
    18841 cgcgcatcca ccccgccccg atggagacct gcgggaccgt cgccgactac gacgtctcga
    18901 ccggcgagat gaccattcac ctgacctcgc aggccccgca cgcccacagg acgctcttcg
    18961 ccatcgtctc cgggctgccg gaacaccaga tccggataat ctcccccgac atcggtggcg
    19021 gcttcgggaa caaggtcccc atctaccctg gttacgtgtg cgccgccgtc gcctccctcg
    19081 ttttgggaag gcccatcaag tggatggaga cgcgttcgga gaacctaatg aactcgagct
    19141 tcgcgcgcga ctaccacatg gtcggcgagc tcgctgccac cagcgaaggg aagatcctcg
    19201 gtgcccgctc gagcgttctc gcgaaccacg gggctttcga cgcccacgcc cagccgggag
    19261 agcagtatcc tacgggtttc ttccccatct tcacgagctg ctacgacgtg ccggccgcct
    19321 acgccgaggc gaccggggta tacaccaaca gggcccctgg cggcatcgcc taccgctgct
    19381 cgttccgggt gaccgaggcc atctacctca tggagcgcac gatggacgtg ctcgccgacg
    19441 agctggggat ggaccccgtg gaactcaggc gcaagaactt cgtcacaaag gagcagatgc
    19501 cctacgtctc cgtcatgggc tgggagtacg acggcggcga ctacgagggc gccctgcagg
    19561 aggctctgcg gatcgccggc tacgaggagc ttctggaaga gcagaggcag gcccgtgaag
    19621 agggcaggat catgggcatc ggactctcca ctttcacgga gatcgtgggc gccggccctg
    19681 gcaagaagtg ccacatagcg ggcatcgaga tgttcgacgc cgcggagctc aggatccacc
    19741 ccacgggcaa ggccatcctc aagctcgggg tcaagtcgca ggggcagggc cacgagacga
    19801 ccttcgccca gatcgtggcc ggagagctcg gcatccccgc tgagaacatc gaggttcgag
    19861 agggcgatac ggacaatacg cccttcggac tcggcactta cggtagccgc tccactccag
    19921 tgtgtggcgc ggctacggcg gtcgtcaccc gcaaggtgcg cgagaaggcg aagaagatag
    19981 cggcccatct cctggaagcc gccgaggggg acgtcgagta tgaggacggg cgcttctacg
    20041 tcaagggatc gccggatcag gcgaagacca tggacgaggt cgccttcgcc gcttacacga
    20101 acgtccccga aggagaagag gccggcctcg aggccgtcac ctactacgac ccgccggaga
    20161 tgacctaccc gttcggcgcg tacatctgcg tggtggagat cgacaggggt accggacagg
    20221 tcgaggtaaa gcgctttatc gcggttgacg actgcggggt caggatcaac ccaacggtcg
    20281 tagacggcca ggtgcacggc ggtctggcgc agggtatcgg tacggccctt atggagatca
    20341 tcgacttcga cgaagagggc aaccacctga acagcagctt tatggactat ctgatcccca
    20401 cctccatgga ggttcccaac tacgagctcg gcgagttcgt gaccccgtcg acgcaccacc
    20461 cgctcggggc ccgcggcgtg gccgagtccc cgaccgtcgg ctcacctccc gccgtggtca
    20521 acgccgtggt cgacgcgctc tcgcccttcg gggtgcgcaa cgtggatatg cccttgacgc
    20581 cgaacaagat ctggaagctc atgcgcgagc acggggtgga ggcctgagac cgatgctccg
    20641 ccaggacccg aactccctcg cccgtcgcgc cgagtctctg agacgggcgg gagaccttgc
    20701 gggcggggaa cgtcccttcg ccctggtcat ggtcgtccgc accgagccgg caacctcggc
    20761 gcgcataggc gaccgtgcga tcgtcttcgc tgacgggagc ctcgacggat gggtcggcgg
    20821 cgggtgcatc gggcccacag cccgcaagga agccctcgcc gccctggagg aaggcgtgac
    20881 acggctcgta cgcgtcaccc ccgacacagc ggcagcccag cccggggtgc gaatggcccg
    20941 catgacctgc gccagcgaag gtaccgcgga cctctacgtg gagcctttcc tcccgcgccc
    21001 aaccctcatc gtcgccggag actcaccggt tgtggcaacg ctcaccgcga ttgcgccgcc
    21061 tttgggcttc aggctgctca ccatggattc aaccacgagg ttgaccccgg gagaggtccc
    21121 ttatccacag gattcgtgga tggtcgtcgc caccttcggc gagtttgacg aggacgccgt
    21181 cgaggcgggt ataaggctgg gcttgccgta cgtcgggctg gtcgcgagcg ctcagagggc
    21241 cgagcaggtg ctctcggagt tgcggacgcg tgggctcgag gagggtgagc ttgcggtggt
    21301 gcgctcccct gccgggctgc cgctgggcac gagcggccag gaaggtatct ccctctccgt
    21361 catggcggag atctcctcgc tgcgcgccga ggcgcgtccg agcttctcgg gggtccagga
    21421 gagagagtcg gccggggaac ccgacacagc agtggatccg gtctgcggga tggcggtcga
    21481 gatggcttcc gccaggcacg tcgtcgagca aggaggcaca cgctactact tctgctgcgc
    21541 aggatgcagg aggaccttcg agcagcggcc ggaaaagtat gaaaccaggg tctccttgcg
    21601 atgagcgtgg tcggcgtggt gctcggggcg gggagatcca gccgtttcgg cgctccgaaa
    21661 cagctcctgc cgttcgggga taccaccctt ctcggccagg ccgtcgggaa tgccaacgcc
    21721 tcatcactcg acaggaccgt cgtcgtgctt ggccgcgcct cgggcgagtt gcgtggcgcc
    21781 atcgacttcg gccgcgcgga ggtggtggag aatacggcct acggcacggg atgcgcctct
    21841 tcgttgctgg cgggtctcga cgccgccgga gacgattgcg atgccctctt tctgctcctc
    21901 ggcgaccagc ctggcgtgct gcctgggttc atagacctgg cgctcgcaga atggagagac
    21961 gaaaggccgt gggctgcggt ctgctcctac aggggtcgcc tcggccatcc tttcgtcttc
    22021 tcccttgatg cgttcgggga tctcagaggt ctgcacggag acaaagcggt ctggaagctc
    22081 atcgaggcgt acccggatag ggttcaggag atcgaactcg acgccgagtt gcctcctgac
    22141 gtggataccc ctgaggacta cgagcgggcg ctcatccacc accgggaagc ctcttagtgc
    22201 tgagaccgtc gggagtatcg gcgatcgtgc tcgccgcggg tggtggtagc cggttcgggg
    22261 gtgggaagct gctcgccgag ctcgggggac ggcccatcat agagacggtt ctccacaacc
    22321 tgcgcgaggc gcccgtcgac gagattatcg tcgtcctcgg cgcggacgca caaagactgc
    22381 gcgaggtctg cgaacgttat ggtgccagga ccctcgcgaa cgaggagtgg gagcggggcc
    22441 agtccacgtc ggtcctcgcc ggactacggg cgtgtggtgg agaagctgcg gttgtcttgc
    22501 tcggggacca gccgttcatc ggggctgagg ccgtcgagcg gctcgtgtct gctttctcgg
    22561 agggagcaaa ggttgcggtc gccacttacg gagggaagcg gcgcaatccc gttctgttct
    22621 cgcgggaggt gtggccgctg ctcgaggcag agttggccgg ggatgagggg gcgcgttctg
    22681 ttcagcggcg gcatcccgag ttggtggtcg aggtgccttg tgaagaagtg ggagaccccg
    22741 cggatatcga cacgagggag gatctgaagc gtctggagga gatgcgcggg cctatgggcg
    22801 actataagat ggcgggagga tagaaaatgg taaccggaag acgtacgata acggttcctt
    22861 aggaggaggt acgatgaaga tcaacaacga gttcacggtg gcggccccga tccagcaggc
    22921 ctgggacacc atgctcaacc tggagaggat cgcgccctgt ctccctgggg cggccatcca
    22981 ggaggagaag gacgaaggcg agtacgacgg cacgatgaag gtgaagatcg gcccgataac
    23041 ggccaactac aagggcaccg tgaagttcga ggaggtcgac gagggtaacc accgcgccgt
    23101 cttgcaggcc acgggccgtg acgcgcgcgg ccagggtacg gcctcggcga ccatcgtctc
    23161 gaccctgcaa gaagaaggcg aaggcaccaa ggtaagcgtc gagacggata tgaaactcac
    23221 cggacgcgcg gcccagttcg gacggggcat cgcccaggac gtggcgacga agatgctcga
    23281 ccagttcgcc tcatgcctgg aggaggagat cacgggcggt cccgaggaag gtgccgcagc
    23341 cgcaacgtcg gaaccggccg gtgaggagaa cggctcgcgt gacggcgcgc agcaggaaga
    23401 agctcctcct gccgccgcag ccggcggcac ggcgggcagg gtcataagca gcgaagaccc
    23461 gaccgtcatg gcgggcggca cggtcgaagg agccgtcgtc gcaggctcag cggaagcccc
    23521 aacgggcgcc ccgaccgccg ggaccgaaca gcagcccccc cgccaagagc gcgaggagcc
    23581 ggaggctttc gacgtcggtg cggcgagcca ggaggcgatc ctcaaacggg ccaaacccgt
    23641 gctcatcggc gccggcgtgc tcctggtcct gatctggctt ctgcgccgcc gccgtcgcta
    23701 ggagttagct ttcagccttc agcggtcagc tttttgtttg ctgatagctg aaggctgata
    23761 gctgaccgct ctaatgaccg tggtgcttgt ggtcgacggc gtgacctttg ccgcgcgaga
    23821 tcaggtgccc gttggggcga aggcgagcgg tccctgtata ggggtggtgc atcctgtctt
    23881 cgctgcgttc gatggttggg gtgtatctgc agggccgtat actcgtgtgg ggtgtagagt
    23941 cggggagagg aggtatggtt gcgttacggg ttcttgatcc ctacggggga tcctcgcacc
    24001 gtcgcggaac tcgcccgtgg ggtggaagag gcgggttggg acggcgcttt ctactgggac
    24061 ggcatctgtg tcggggacat ggaaacgtac gatccatggg tggtgatgtc tgggatggcg
    24121 atgcgcacgg agagggtgag gatcggggcc gtgctgacgc cgccggccag gaggagaccg
    24181 tggaagctgg ctcgcgagac ggtcgccctc gaccatttgt ccaacgggcg cctcgtattg
    24241 ccggtaggac tcggggccct ggacgacggt ggtttttcga aggtgggtga accgaccgac
    24301 aggaaggtta gggcacagct tctcgacgag agcctggata tcttgaccgg gttgtggagc
    24361 ggggagccgt tctcttacgc gggcgagcac taccggctcg aggagatgac attttcgccg
    24421 cctcctgtcc aggagccgcg tatccctata tgggtggtag gagcgtggcc gagcaggaag
    24481 tcgatggggc ggacgctacg cttcgacggg gtgcttgcgg ccgcggttgg cggagggccc
    24541 gaatcgccgg gcgttacgcc cgagaccatt cgtgagatca gggattacgt cagagagaac
    24601 agagaagacg acggcacctt cgacatcatt tgggagggac agacgcccgg tgaagacccg
    24661 ggtcgggccg cctccatcgt gcgtcccttc gcgcaggcag gggccacctg gtggatcgaa
    24721 tccccctgga tgcctcccaa cgagccggac gacctgcgcc gccgcataga acagggtcct
    24781 ccgagcgtcg actaacgacc caaaaagctg accgctgaat gctgacagcg gcgaggttcg
    24841 cgggccaccg cgaacctcgc ctacctacgg gcatctttga ggatctcgaa gacctcttcg
    24901 atcagggcct cgcgtttgtc gggcgggagg tcgcgggggt ggatggtttg tttgtcggac
    24961 tggaggttgc gcaggctagc ctcttcggcc agataccggt ctataaggcg gactatggcg
    25021 tcaaccttct cccggggcag tccgtcgccg ccacgggtca gtctgctcca catgtctcat
    25081 gtcccttccg tgaaggtggg cggtgtcgcg gcagattata tcgtcggggc attttcaagc
    25141 ccgcctggag gccgtgcggc gtggaaaagc gcggtcttcc gagggtatga agccgcttcc
    25201 ctggcgccag gtatgggcag ttatgtggat ctcgtggtct tctatctcga cgacgttgaa
    25261 gctagatgcg cgtccgggac ggatccgatt cgagatggtc cccgcggtcg agatgaccag
    25321 accgtgggcg cccctctcga tggtgtggat ggcgtcctgg tgatcgtgcc cgcagaggat
    25381 caactcggta ccgagatcgg cgaaggcgtg gagggcttgc tccgtattgg cgaggccgtg
    25441 gcggcctgat tgctcgcccc ttatggggtt gtggtgcatc atcacgatgc gggccgaggt
    25501 gggggcggcg tgctcgaaga tccccctcac acgcatcacg tcggcgtgcc tgacctggcc
    25561 tataacgccg aggtcccgca agcggcgggt gatcgaaccc cgcgtgatgc cgtgggccgt
    25621 gtttacaccc gcgatgacca cccctggtac ctccagagaa gggctcaggt cttccgagat
    25681 gtgcctgacg tacctggagt acttgaactt atgggcctgc tccctgaaga gaccggcgag
    25741 tcccaggttg cgggccaccg ccccgagcca gcggatgtcg tggttgccgg ggacgacggt
    25801 gtagggtgct atcttcccga gggagtccag gtaggcgcgg gcgttcgaaa actccccgtt
    25861 cgtgcagcgc tgcgtcaggt cgccggagat ggccacggcg tctggttgga tcatttcaac
    25921 ataccccttc agggcgtcga ggcgttcggc gaccgcggga cgcccgaagt gtaagtccga
    25981 aatgtgcacg acggtggcca cgaacagcga ttctacccga acgcaccccg gctccaaacc
    26041 ctgccctacc tctactccag aggtaggacc tgtatctagc cgatgctctg caggaaatcc
    26101 gcgaggacag cgtcgaactt ctcgggggct tcgaggttag ataggtggcc gacccgggga
    26161 atggtcacgt ggcgggcttc gggtatcttc gctgccatgg cgcccatgac ctcgggggag
    26221 gagatgccat cttcctcccc gccgacgacc agggtcggaa cgccgatctt tccgaggagc
    26281 ggggtagagt ccgggcgctc ccgcatggcg ccgagggcgg cgacgacgcc gtcgggggag
    26341 ttctcgaaga tcatggcccg cacgttctcc acaagctcct cgtcgttccg taaggtgtcc
    26401 ggggcgagga ggcgttccat ctggagttct acgaggaccc cgaccccctc ctcggcgacc
    26461 ctgagggccg tctcgttgcg ggtctccctt atctcctcgg agtcgggttc ggcacgggtg
    26521 ttggcgagga tcagggccct caccctctcg ggaaagagcc tcatgcactc gaaggcgacg
    26581 tagccgccca tggagacgcc gccgaggatc acgcgttcca gatgcagcct gtcgaggagg
    26641 ccgcgtactc cttctgcata gtaatggacg tcgggctggg ccggagtacg gggagaacgc
    26701 ccgaatccgg ggtaatccgg cgcgacgacc cggtggctcc ccgagaaggc ccccatctgg
    26761 ggctcgaaca tcctgccgtt gagcgggaaa gcgtggagca ggacgagggg atcaccctcc
    26821 ccgaggtccc tgtacgacat cgccacgccg gaagtcctgt gatccactac gctctcctcg
    26881 tttaggcttc aagttacagg tttcaggtcc cgcataacct tctccctgcg agaccatcgc
    26941 cgccaccgat ggtagcgttt tcgacggttg atttccgcgt accacttttc cctgatacct
    27001 gatgcctgat gcctgaaacc tatgcatctt ccgctactat atcttctgac ggttcccgag
    27061 cgaggggtaa cgaacatgtg gaagaacctg gcgagcgtga tggaagggga gatcgtacgt
    27121 ctggagccgc tggcgcatcg ccacgaggag gggctcttcg aggtcgccca ggatgggagg
    27181 atctggcgtt ggatgcccta tgatcctagt gggtcccgcg agagttttcg ctcctggctc
    27241 ggggatgccc tggctgcttc ttcggcaggg acggaggccg ccttcgccac cgtgaacgta
    27301 gccacagggg agcctgttgg cagcacccgt tatctcgccc tccgtcccga gcaccggggg
    27361 ctggagatag gatggacctg gctcgcgcca gcacgctggc agacgggcgc caacgtcgag
    27421 gccaagctcc tgatgctcga tcacgccttc gggcgcttgg ggtgcttgcg cgtcgagttc
    27481 aagaccgact cccgcaacga gcgttcgcgt gcagccctgg cggccctccc ggctcggttc
    27541 gagggcatct tccgcaagca catgctcgtg cgcgacggcg aacgtcgcga ctccgcctac
    27601 tacagcatcg tcgacgacga gtggccagag gtcagggaaa acctaagccg gcgcatcgac
    27661 gccatccgaa aagaggaggc cacatgagcg aaggcggaac agcggcagga cgacgcaggg
    27721 cgacggtcgc cgaggcgatg gggcggttac ccggccccga gggagagcgg ttcgcggagg
    27781 tgctcggaca cgggagcatg gaggtcgaga tctacgcgcc ccgcggcacc gacccgcaga
    27841 agccgcatac ccgcgacgag ctgtacgtgg tcgtcagcgg gagcgggacg ttcgtcaacg
    27901 ggccggagcg cgatcccttc ggccccggcg acgtcttgtt cgtgcctgcc ggcgtcgagc
    27961 accgcttcga ggacttcacg gacgaccttg cggtgtgggt cgtcttctac ggccctgaag
    28021 gcggtgaggc cggatgacca tgcgtctaca cgaccacctg ccttcgggga acggctacaa
    28081 ggtaagactg ttgatggcgc aactcggcat ccccttcgag cggatcgagt acgacataac
    28141 cgagggcgag acgcgaacgc ccgagttcct cgaaaacgtc aacgctaacg ggcgcatccc
    28201 cgtgctggag accgaaacgg gagagttcct gccggaatca aatgcgatcc tgttctacct
    28261 tgcggaaggc accccgtttt tgcctgacga ccggtttgac agggcgcagg cgctgaggtg
    28321 gatgttcttc gagcagtaca accacgagcc aaacatcgcc gtggcccgcg cctggctcca
    28381 cgtcttccac acggagatga ccgaagaacg cctgggtgcc ctcgaggtca aacaaaagct
    28441 gggctacgac gctctcgggg tcatggagga gcacctggaa ggcaggctgt tcttcgtcgg
    28501 ggggcgttac tcggtagcgg acgtcgccct ctacgcctac acccacgtcg cggatgaggg
    28561 gggcttcgat ctcgaaggct tcccggcggt cagcgcgtgg ctggagcgcg tctcgtcgca
    28621 gcccggatac ataccgataa cccagggctg aaaaacaacg ttcggctatc tggagaggag
    28681 ctcgcatgac gaccgaacta cgaacctcgc atccctacca catgcacgag gcgatcctgg
    28741 cccaacccga ggcgctgatc cgggtcctcg agcgcaacga aagcgcgatc gacgagttcg
    28801 cgactcgagt atctctctgc gagagggttt tcctggcggg tatcggaacc tcacaccacg
    28861 catccctggt cggtgagcac ctgatgcgat cctacggcgg cgggcccgac gtccgtgcgt
    28921 tccactcctt cgacctcgcg ctctacgggc cggagttgcg ccgggaggac tgcgtcgtcg
    28981 ctgtgagcca ccgcggggcc aaacgctaca cggccctcgc gctggagcaa gccagggaga
    29041 agggctgccc gacggccctc ataacgggcg aagcaggctc cggtgaaggg cgggccgacg
    29101 ccgtgttccg gacggtggct caggagagct cccccgccca caccgtcagc tacacgacag
    29161 ccgtggcctt gctcgcgctt ctcgctggca gggtcggata ccacaggacc ggctcggcca
    29221 cgctgcccta tggactcctg cgggaggagc ttcccggtgc gctgcgggcg gcgctcggta
    29281 cagaaggaga agtggctgcg ttcgcgcgcg agcatgtcgg acggcgcagg atctggcttc
    29341 tcggcggggg gccgagcgcg gtaacggcca ccgagacggc gctaaagatc aaagagacct
    29401 cctacctcca ggccgagggc atgccgacgg agacgatgct gcacggcccc ttccagtgcg
    29461 tcgaggcgga cgacctgttc gtcctcatag ccccttcggg cgccgccagg gaacgtaccc
    29521 tggaggtggc agagctggta gaagaagtgg gagctccgta cgtgatcttc ggcgacgaca
    29581 cgaccgaggg ccgtccggcc gccgcaggcg tgctgcgggt gcccgaagta cccgagccgt
    29641 tcagcgccct tacctgtctc gtgccgttac agctctttgc ctaccatctt gcgctcgcgc
    29701 gcgggaccaa ccctgatagc ttcaggggag acgacccgcg cttcgcgagg gccgacatct
    29761 ccgctcgttt gtaggtagaa tgggcgcctg tgcgtctgat ctacaccctt ctgctcgtcg
    29821 ctactacggc cgtttggggc tggacgtttg tcgtggttca ggatgcgatc tccgtctacg
    29881 gcgtaatccc gttcctggcg gtccgcttcg cactggccgg tgccgcgctg gcccccatct
    29941 acgctatgaa gctcacgcgc cggtcgctga tcgtcggtgg cggcatcggc gtggtgctcg
    30001 ccgccggcta tctttttcag acgacaggcc tgctcttcac gacgccgaca aactcgggcc
    30061 tcatcacggg tctcttcgtc gtcttcgcgc cgctcgccga ccggttcctg ttcggggcca
    30121 acgtctcgcg gcaggtcgta ctggccgtcg ctttgagcct actcgggatg gtcttgctag
    30181 ccggtggcgg gcccgagggc gcgaactggg gagacctcct gacgctcctg tgcgcggcgg
    30241 cgctcggtct gcacatcgcg ctcctctccc gctatgccgc ttcctacgac gcgggtgcgc
    30301 ttacgctggc ccagatcctc tccatagcgc tgctcttcgt catagtgtgg ccgttcttcg
    30361 ctcccgtctc cttgccgacg cccgaagtat ggttcgcgct gctcgtgacc ggactcttgg
    30421 catcggccgg cgctttcctg gtgcagacca cggtgcagca gcacatcccg gcggcgcgga
    30481 ccgcgatcat cctgaccatg gaacccgtct tcgccgcgct ctttgggtac tggctcgcgg
    30541 gggacaggct ggtggcggtc cagatcctcg gcgcgctcat gatcctctcc gccctcgtga
    30601 tcggagaggt agtacctgtg ttgcggcggg gcaagagccc cgtccaccac gattgagcat
    30661 cgcccgctag tccggctctg ttcccagaat ggacctgata cctgtagcct ggaacattcc
    30721 aagcggtaca ctagatgcaa gtctgtgatt tgaggaggtt cgagtggcac acacagtgaa
    30781 agtccctgag tacgaggtac aggatggcgt tcgcgtagac ccgagccgga cggctttgat
    30841 cataggggac atgcagaacg atttcgtgac ggagggtggc acgctggtcg tccccggcgc
    30901 agaggaaacg ataccggcta tacggaacct gctggagctg gcgcgggagt ccggcatgaa
    30961 ggtggtcttc gtccaggaca ctcacaacga cggcgacccg gagtgggaga tctggggcga
    31021 gcattgccgc gaggggagtt ggggctggca ggttgtcgac ggactcgccc ctaacgaaga
    31081 cgaggcgact atacgcaagc cgcgctacga tgccttctac ggaacgggac ttgagcacat
    31141 gctgcgcctg tgggacgtgc atacgctcat catctgtggc acggtcgcca acatctgtgt
    31201 ccattacacg gcgtccagcg cagccatgcg gtggttcaac gtcattgttc ccagggactg
    31261 catctccagc ttcaacgact tcgatatgga ggcatcgctg agccagacgg ccgtcctcct
    31321 cggtgaagtg acaagcagcg aaaacattca tataggggta ccggagcccg aggagatcta
    31381 catgagcaag ctggaagagg cggccacgcg cgagcggggc gagagatagg gagaccctgc
    31441 gcggcaacgt ggacatccag ctaaacgcga gcctggccta gcggccctcc actagagggc
    31501 aatacaggtt caggggtctg acagcgtgct cagggcttga ggcgggccat ggagtaggcg
    31561 tccacgtact cgccctttct gaaagcaaag cggcggtgtg tgccctcgac ttcgaacccg
    31621 aacttctcat acagggcgat cgcgggggcg ttgtccacgt agacgctcag ctcgatgcgc
    31681 gtgaggttta gccagttatc cgtgaggtcg agcgccgcct ccatcaacgc cgtcccgacg
    31741 cccttgccct gccagtcgtc acgcacggcc atcccgatgc cgccaacgtg acgcatgcgc
    31801 cagcgggtgg gggaggtctc caggccgagg ctccctacca cttcgtcctc cacgcaggcc
    31861 accagcgaat acaacccctc cggtggctcc gagagacgtt tgcgccacgt ctctgccgaa
    31921 gggaggggca tttgcaaagt acccgctatc gcctttgggc cgctgaagat gcggcgtagc
    31981 gcctcgtagt cctcaggctc cgagtgacgg atggttatct ccaaatgcgt cctcagttta
    32041 cgtacccttg cgggtagaac aagagccccc gccggtatgg gtgggggctc cgttcgcgct
    32101 ccgtggctcc gagattacgt ggtgcgtggt tcttcggccg gggcggactc ctggagctct
    32161 tgttctatgt tcgagatgcc ggtgagggag cggacgtaga tctcatcgta atcggtctgg
    32221 agaccctgct cgcgttcgcg ctcggcgccg cgcccaccca cgagcgtgcc caggatgcag
    32281 cccaggatgc ccagaggaac cgagaacagc gccgggtaca caaacgggaa gatggggtca
    32341 atggggatga tcggggtcaa cggaggctcc gggttcggcc cggtcatcac gttggggctc
    32401 aggatgatca ggacgacgca ggagatcagg ccgaccagga tgcccgtcac cgcccctgtg
    32461 gtgttgaacc gcctccagaa gatcgtcagc aggatcaccg gcacgttggc gctcgccgcg
    32521 acggcgaacg cgagcgcgac caggaacgcc acgtttagat tctgcgccgg tatcgccagg
    32581 agtatcgccg ccacggagat ccccacggcc gtgaagcgcg ccgcacggaa ctgctcttgc
    32641 tccgaagcgt tgccgccccg gatcacgttg gtgtagaagt cgtgggcgaa ggcgctggag
    32701 cccgccacga cgagcccggc caccacagcc acgatggtgg cgaaggcgac cgccgagatg
    32761 aaggcgaaga agatggggcc gccgagcgct tcggcgagct gcggggcggc cgtgttcccc
    32821 gccgggttct gctgcgcgat ggcgttctgg ccgacgaaga gcgccgcgcc gtagcccagg
    32881 acaggtgtca gcaggtagaa cagccctatg atccaggtcg cagtgacgat cgaggaccgg
    32941 gccgtcttgg cgtcaggcac cgtcaggaac cggatcaaga tgtgcggcag ccccgccgta
    33001 ccgagcacga gggcgatgtt gagcgagatc acgtcgagcc cggcgacgag atcggtcggg
    33061 ggcggcggct tcaccatgtc cgagcctatc tgcgagctaa cctcgttgaa gaggttgacc
    33121 gggttgaagc cgatgctcgg ctgggcgagc acgaagaggg acagggtcag ggtgccggcg
    33181 atgagcagca cggccttgac tatctggatc caggtcgtcg ccaccatgcc gccggccacg
    33241 atgtagaccg tcatcaggat gccgatgatg atgaccgaga ggacgtagtc caggccgagc
    33301 agcagctcga taagcgcccc cgcgccgttg agctgggcga tcatgtagaa catgctgatc
    33361 gctatggtcg agagcgccgc cgccgaacgc acgctcggct tgttgaaccg gctagccagc
    33421 atgtctgcaa gcgtgtactt gcccaaattt ctcaagggtt cggccaccag cagcagcacc
    33481 accaggtagg ccacgaggaa gcctatcgag agatagaaac ccgagaagcc ggtgagcgag
    33541 atggcccccg cgatccccag gaaagacgcc gccgagaggt agtcgccgga gatggcgagg
    33601 ccgttctgcc agcccttgat cccgccgccc gccacgtagt ggtcgctggt tgatttgttc
    33661 tggcgcgacg cccaggcggt gatgccgagc gtcagcgcga tgatcaggac gaagaagatg
    33721 atggatattg cctggtcgct cactccgacc cccccgttcc gtcggtccta ccaccctcat
    33781 cgacctccga ggcttcgtgc ctggcacggt ctaccagatc gtcccacccg ttggcccggc
    33841 tgacgtagag gtgcatgagg atccaggtca tcgcgaactg cgcgaaagcg tagatccaag
    33901 ccaggtttat cgccccgatg acgttcacgt ccaacaccgt ggtgaacgcc gtcagaaacg
    33961 gcaaaccgaa gtagaagaca aagaagaaga tcgtcgccgg taaaatgaat gcctttcgct
    34021 tctgtgtcag ctccctgaac gccgacgtcc gctcgatgcg gacccactcc tcgtgcctct
    34081 ccacgaatct cctttcaccg gattttccct gccctcttgc ggcgcactat attgcatgcc
    34141 ttatacacgg tcacacatat atgaacgaaa agttgcaaga cttcctcgga acgcgtctct
    34201 ggacgccgtt atcagaagac tcttgaatag ctggcgcggc aagaccctcc ttcgagaagg
    34261 agggtcttgc cgcgcccgag aagattgcct aagctttctc tatgctcacc gcgcaggctt
    34321 ttagctcggc cgttccggac tgcgggtcga cggcgttgat ggtgaggacg ttggtcggga
    34381 cctgatccgg gaagctgtag cccatgaata gaagccctgg cctcaccctg tcggtgacac
    34441 gggccttcac cgcttcgacg gtcccgcgcc gggagctgac ccgcaccatg tcgccatctt
    34501 cgatgccgtg cccttcggca tcttcctcgc agatctccag cagctcttct gggtctttga
    34561 ccttcttgta gtcctgggtc atggtacccg tgttgtggaa ggcgaggcgg cgtccggtcg
    34621 tcaactggaa ggggtactct tcgtcgggca tctccaccgg tccctcgtgg tccacggggg
    34681 tgaagggtgc ccggttctcg acctcctcgg ccctgaggtg ggcgtgcagg aatttggtgc
    34741 ccacatgggg cttctccacc acgtcggtgg tgatcgcctc acccttggca ttctcggcgc
    34801 ccctggtgct ggcggccagc tgctccttgg gggtgttgtc cgagatctct tcgaagctac
    34861 cgtcgaggca cggccactgg ataccaccgt gctgctccaa ccgctcgtag ctcataccgg
    34921 agaacttggg tgctagcctg cgtatctctt cccagacatc ttcgaccgtc tcgaagcccc
    34981 agtcgtaacc catgcggttg gcgatgtcct gcacgatcca ctcgtccggg cgtgcctcgc
    35041 cgggtgggtc taccaccctg tggacccgct gcacccgccg ctcgctgttg acgaccgtgc
    35101 cctcggcctc ggcccagctc gcggccgcgg gcagaaccac gtccgccata cgggcggtct
    35161 tggtcatcat gatgtcctgc accacgagca ggtcgaggcc gccgaagagc ctgtcgtggt
    35221 agtgttcgtc ggcgtcggac tgtgccgggt tctccccgat aacgtacatg cagcggatat
    35281 caccacgttc cacggcgtcc atcatggcgg gctggtgctt gccgaccttc tcgggcaacg
    35341 gcgaccccca tacctcctcg aatagctcgt gggcctcggg gtcgtcccac ctccagccac
    35401 cgaccatctt gtccggcagt gcccccatgt cgccgccgcc ctgaacgttg ttctggcccc
    35461 gcagcgggtt caggccgcta ccgtacttgc cgacgtgacc ggtaagaagc cccaacccga
    35521 tgagcgcgaa cacgccatcg gcgccgttgt ggtgctcggt gatgcccagg gtccagttga
    35581 ggacagccgt atccgccgtc gcgtacatgc gggcgacctc gcggatgtca tcggccggga
    35641 cgcccgtgat ctgctcggcg tactccggcg tgtagccgga gaccttatcc ctgtaagcct
    35701 cgaagtcttt cgtggcccgc tcgatgaact ctttgtcgtg gaggtcctcg gcgatgatca
    35761 cgttcgccat cgcgttcgcc agggcgatgt ccgtacccac gttgaggccg agccacttct
    35821 cggccttctt ggtctgatct atctttcgcg ggtccaccac gaccatcttc gcgccgttct
    35881 ttatgccgcg gcgcatgtgg ttgtagatga tggggtggca ctccatggtg ttggagcccc
    35941 agcagattat gaggtctgtg ttctcgagct cctcgtaaga ggtggttgct ccgcccgctc
    36001 caaacgttgc caccagaccg gtgacgctgg cggcgtgtca agcacggtta catgagtcta
    36061 tgttgctgtt cccgaggacc tggcggacga acctactcgc cagatagttg agctcgttgg
    36121 tcgagcgcga ggagctaaag agtccgaaag cgtcgggccc gtgctcttct ttgatctcgc
    36181 tcatcttgcc ggcgacgaga tccagagcct cgtcccagga agcaggaacc aactcgccgt
    36241 ctttgcgcac caggggtcgc gtcagcctcc tgtcgctgta gaggtggcgc cagccctcgc
    36301 ggcccttgat gcaagcctcg cctttgctgc tcgggttctc cttgtctccg gcgactttgg
    36361 taatgactcc gccctcgacc ttgacggaca acccgcagcc gacgccacag aagccgcaga
    36421 tcgttgtcag ctcgcgcgcg ccgttggcct tgccattctc gtgtactctc tccgctgtgg
    36481 ccataaccct cgtcctaccc ttcttgtagc gaagctgctc tgttcttgca ttctaacttc
    36541 gacggccgcg tttgcccaac acggccgccg gttgacacac gcaaagcatc aacgtaacat
    36601 ccgcgatcac agatacgtcg ggggatcgag actgagaagg gggcagtcat caacgggttc
    36661 ttgtctaggg agaggattac cgccgggcct ggcttcaaca ggtggcttat accgccggcg
    36721 gcgctgttgg tgcacttgag cataggcgag gtctacgctt tcagcgtgtt caagaacccg
    36781 ctggtggagc ggttcggggt gggcctgacg ccgatcggga tcatcttcag catcagcatc
    36841 gtgatgctgg gtctgtcggc cgcggtgttc ggcacgtggg tggaacgcgc ggggccgcgc
    36901 gcggcgatgt tcaccgccgc cctgtgctgg tctagtgggt tcgccatcgg ggcgctgggt
    36961 gtggcgacca cgcagttgtg gctcgtctat ctgggatacg gggtagtggg cggtatcggg
    37021 ctcggcatcg gatatatctc gccggtctcg acgttgatca agtggttccc cgacaggcct
    37081 ggtctggcca ccggcctcgc catcatgggc tttgggggcg gcgcgctgat cgcctcgccg
    37141 ctctccagca cgttgctcga aacctacgcc agcaacccgg tggatgcgat cgtgccctcg
    37201 ttcctgacgc tgggggcctt gtacctggtc tcgatgatga tcggcgcgtt cgtggtgcgc
    37261 ctgcccccgg aggattggca gcccgagggc tggactcctc ccaccgagga ggagacaagc
    37321 gacctgcaga ccaccgccga cgtgtcggcg agcagctcga tccgcacgcc gcagttctgg
    37381 atgttgtgga cggtgctctt ctgcaacgtg acggctggga tcggcgttct ggagcaggct
    37441 tcgccgatga tccaggactt cttctccggc attacgcccg ccgctgcggc gggcttcgtc
    37501 gggctgctgt cactagccaa catggccgga cggataggct ggtcctcgac ctcggactac
    37561 atcggacgca agcccatcta catggtgtac cttggcgttg gcgtggtcct ttacttcgtg
    37621 ctggccgcct tcggggcggc ctcgctagtg ctgttcctac tggtgaccat ggtgatcctg
    37681 agcttctatg gcggcgggtt tgccacggta ccggcctacc tcaaggactt gttcggtgtc
    37741 gcgcaggtgg gggccataca cgggaggctc ctcacggcgt ggtcggccgc cggcgtcgcg
    37801 gggccgctga tcatcaacgc catcgccgac taccagattg ccaacgggcg cacaggggcc
    37861 gacctgtaca cgctctccct gtacatcatg gtcggagtgc tggcggttgg cttcgtcgcc
    37921 aacctgctgg tccgacccgt agatccgagg ttctacatgg agcctgggcc ggaaggtgag
    37981 cccgagaagg aatccaccgc ctccggcagg gccgccggca gcgaatcgta ggaggaacac
    38041 catgagcgaa gaagcgcgcc aggagaacgg aggacaaacc tcctcctcga gcacgatcct
    38101 tgtcgtcgtg ctgtgggcca tggtcctgat accgctcgcc tggggcatct accagacgct
    38161 tttgggtgtc gtcgccctgt tcacggggta gcgacctctg gagcggtctg cctgtggggg
    38221 cggttcgcga accgccctcg ccgcacccct gtgtaaccta tcctagatcc ggatgcgctc
    38281 gcttccggca tagacgttga atctacagtc gcgcaggaag ccgaccagcg tcatgccgaa
    38341 ctgcctggcc acatcgacgg cgaggctgct gggggccgag atagcgcaca ctacgggggc
    38401 cccggcggcc agggacttct gcaggatctc gaagctgctc cggccgctga ccatgacgat
    38461 gtgatccgtg agcggcagcc tgccctccag gagcgcccac ccgacgagct tgtccatcgc
    38521 gttgtgcctt cctacgtctt cccgcagcgc gataagctca ccttcctcgt cgaagagggc
    38581 cgcagcatgc aggccgcccg tcgcgtcgaa gagcccctgc gccgccctca gcttctcggg
    38641 cagggtggtg aggacctcag ccgaaacctc gggaccggcg gtcatcaccg ggcagccgcg
    38701 cagttccaac tgctccaggc tggccttgcc gcacacgcca caggcgctcg ttgtgtagaa
    38761 gtgcctctcc agcggcctga ggtcgaactc acgcccgccg cgcagctcga cgttcacgat
    38821 gttgtacctt tgctcggcgt cgaggtcgga gtctacgcag taactgatct tccgaacgtc
    38881 gtcgggagag tcaatgatgc cttctccgta caggaatccc gcagcgagct cgaagtcagc
    38941 ccctggggtg cgcatcgtaa cggcgacggt ctgcgtcgtc tgcccggcca ggagccttat
    39001 ctccatcggc tcctctgtcg ctagcgtgtc gggccgcacg cgcatccgac cgtcctccac
    39061 gacccgtacc cgcgtcttcg tcttgctccc gcgcctgact ctgtccgagg tcttctccag
    39121 catcggcctc tcctggtccg gtcttactta gagtgtatca gcggcagcgt gttagcatgc
    39181 atggacgatg aacgagcgga tcgggcaacc agattgagat tgatgggcct cccgaacgtc
    39241 gctcgctatc ccgaggcgac cgtggccagg gacgagacga gcatcctcat ccacttcgcc
    39301 ggtccctacg gggagcagaa gatgagcgtc ccgctagggt acgttggcgg ggacgcagag
    39361 gcggccgagc tctggctgct cgcgcagcta caagagatcg gctacagcgt caggcgtggg
    39421 gagccgggtc cctcagcccc gtaatggcta tcttgctggg gtgaagcatc tcctccgtgc
    39481 ttacccgtgt gagtaacgat tgttatactt gcaataggtg ctaggcggtt cctgtcgggt
    39541 agaggatcac cggcggcatc cctgaggttg cgatcggcat aagattcagg agcgttgtct
    39601 gagatgccgg agacgccaga gctgtctcgg aggaacaccg cagttgacct tggtgagtcg
    39661 gtagagttcc caatgacgcc cgggcgcacg agccccaacg acaacctgcc gctccacctg
    39721 agtagcttga tcggacgcga gcgagagact gcccaggcca agaggctgct ggaccatcat
    39781 cgcctcttga cgctcaccgg accgggtggc tcgggcaaga cgcgcctcgc cctggcggtg
    39841 ggggagttcg aggacggggt gtggtgggtg gccctcgcct cactctcaga tccggagctc
    39901 gtggggcagg cggtggcctc ggtcctggag gtgcgcgagc aaccgggtcg ctccttgggc
    39961 gagacgctcg cggaccatct tgcatccagg gagacgctcc tgatcctgga caactgcgag
    40021 cacctcatag aggcttgcgc ccggctcgcc gacgccctgc cgcactcctg ctcgaaccta
    40081 cgcgtcctcg cgaccagccg cgaggcattg ggggtcgcgg gcgagacggt ccttttagtg
    40141 ccaccactat ccttgccgga ttccggtcat ccccctcccc cagaagagtt ggagcgctac
    40201 gaggccatcc gtctattcgt cgaacgggcc agatacgcaa gcccgagctt cgagctcacc
    40261 gggcggaacg cctcggctgt cgcgcggctg tgccgaaacc tggatggcat cccacttgcc
    40321 atagagctcg cggcggcgag gacgagggtg ctctccgtgg agcagatctc ctcgcgcctg
    40381 gccgacagct tccggctgct gaagagcgaa agccgtacgg cggacccccg ccagaagacc
    40441 cttgaggcga ccctcgaatg gagccacgag ctgcttggtg agaaggagcg cgccctcttc
    40501 cggcgtctgt cggtgttcgc cgggggcttc accctggaag cggccgagga ggtttgcgcg
    40561 ggaggggaca tcgaagaaga cgccgtgctg gaagtgctct cggatctggt ggaagtcgtt
    40621 ggtgttggtc accgagcggg atggggagga ggcgcgctac cggctccttg agatggtcag
    40681 gcaacacgga caggagaagc tagaggagtc cggcgaagcg ggagagttac ggcttaggca
    40741 cgccaagtcc ttcctcggac tggccgaaca ggcggagcct gaactcaaga ggcgccagca
    40801 ggtggtgtgg ctggcgcgcc tggagaggga gcacgacaac ctgcgtgccg ccatgcgatg
    40861 gttgctagaa gagggcgaaa cggagaccgc cgtgcagttc gcgtgggtga tgtggctgtt
    40921 ctggtggtac cacgggtatc agggcgaggg gcgtcgctac gccgaagcga tgttggagag
    40981 agacgacgtt cttagcgccg acgtacgggc aagagcgatc tacgtcagag ccgccgtctc
    41041 gtacggactg gagagcatgg aattgacgat gcggctgtgc gaagagagcg ccgccctgtt
    41101 cagcttcgcc ctcgttctgt ggatagggaa cccgcatgaa atcaccggca agagagtacg
    41161 tcaccctcgt cgaggtgctg gagctgaccc gacccttcga ggagttcgcg gacgccatcg
    41221 aggccgcctc ccgaagactc gaagcggaag gggttcagga actgatcgcg gtccacttct
    41281 acggtagccc ggaatcaacc gaagcgggcg cgatcctcac cttctcggat cgggatcgca
    41341 tgatcgatca catcgagatg atctcggggt gggaggagtt cgagaggttc ttcgccaccg
    41401 tcaagcctct ggacgtgcgg gtctacggca ggctgagcga ggaagcggag gagtggatcg
    41461 ggcggttcga cgtgcttagc aagaagttcg aggatcacgt cgcaggcttc gtgcgttgag
    41521 cgtccaagaa caaagcgggc tctgttccgg tcgagactac cttacggcgc caggctcgct
    41581 acgcttatgc gcccagttgc gcaccctctg gagccagacc gtgcgcctgg tggtcttgct
    41641 cttctcgacg aagtcggtga gaacgccgtt cgtgacgggc gggaggacga tgctctcggt
    41701 tgtctctgcg tagtcggagc cttcgagaat caggatcgtc atccgttctc cattgtagcg
    41761 ccagacctcg gggacgccta tctgggcgta gatggggagc ttgttaaagg aaggactggt
    41821 gatatctatc tcgatcacca ggtccggtgg cgggtcgacg gccagatcta tgcgttcctt
    41881 gccacgaacc tgctcctcgt tctggatgta gaagcaggag tcgggttcga agccgcgctc
    41941 taggtcttcg cgcctgaagg tcgtcgagcc gagatcttcg gcttcgatgt ctagctcctc
    42001 ggccacggcc aacacgagtt gtgctatgcg tcggttgaac ttctcatgtt cgggtgacgg
    42061 gctcattatc tccagctctc cccgatcgta agtaaaacgc ggcgcgctat tgttctcatg
    42121 gtcggcgagc agacgttcgt aggtgttcca acctatgtta tgaagaacca ccctctgctc
    42181 cgccggagtc tttaccgttt ccatcgaata ctcttttcga cgctcacgtc gtaattctat
    42241 agcgtaccgg agacaggaaa cggggaccgg gctgcttcag ccggtgacgt tgccccgcgc
    42301 ccactccctt accctgcgaa gccagtccgt gcttcctagc gaggtgctct cttcgacgaa
    42361 gtgggtaaga tcgccgcttg tgagcgactg caaagctacg ctctccccgg cttcaacgta
    42421 ttctccctct tccagcaaga agatggtggc acgggttcca tcgtagcgcc agacctcgtg
    42481 gacgcccaaa cgagcgtaga tcgggaactt gtcgagagag gggctggtga tgtccacgtc
    42541 gataaccaat ctggtggcgg gtccacagac aggtctatcc tgggcttgcc gcggatgcgc
    42601 tcctcgttcc tgatgtagaa gctggcgtcg ggctcgaaac ctcgcccgat atcgccgtgc
    42661 ctgtaggtcg tagagcctac gccgtacgcg ttgacgcgca tctcttcggc gagcaccgcg
    42721 acgagcagcg cgacgaagta agctacactc tcgtgctccg aagaagggct catgatctcc
    42781 aggtctcccc ggtcgtaggc caaccgcggt accctgctgt ctcctcgctc atcaagcagc
    42841 cgctcgtagg tttcccagct tatgttgcga aggagaaccc tctgttctgc ccgactctca
    42901 accgtttcca ttccacaacc ttctcggatc gatcgcgacg aaagtatagc gtcgagctcc
    42961 ggtgaagtta ggaagagggg ccggagagat ccgacccctc ggaatcgtcc tcgttgtcag
    43021 tggtgctacg cgttgatgta gccgttcggg ttgaggacgt acttcttggc cgcgcccttg
    43081 tcgaagtcct tgtagccttc gggggcctcg tcgagggtga cgaccgtggc gttgacggcg
    43141 tcggcgatgt gggccttatc gtgcaggatg ctcatcatca ggccgcggtt gtacttcatg
    43201 accggacact ggccggtgaa gaaggcgtgg ctcttggccc agccgagtcc gaaccggatc
    43261 ttgagcgtcc cctcctgggc gtccggatcg gctgctcctg ggtcgccagt cacgtacagg
    43321 ccaggtatgc cgagtgagcc gccggcacgc gtgatgcttt ggacggagtt caggaccaca
    43381 gcaggcgcct cttcgccgcc gcgtccggac gcctcgaagc cgacggcgtc gaccgcgcag
    43441 tcgacctcgg gctcgccgac gatctcggcg atctggtcgg gaagctccgc gtccccgccg
    43501 acgttgacgg tctcgcaacc gaacgaacgc gcctgcgcca gccggtcgtc gttgaggtcg
    43561 ccgacgatga cgacggccgc gccgagtagc tgcgccgagt aggccgccgc gagccccaca
    43621 gggcctgcgc cggctacgta gactgtcgaa ccggtcgtga cctgggccgt gtaggcgccg
    43681 tggtagccgg ttgggaagat gtccgagagc atggtgaggt caagaatctt ctccagcgcc
    43741 tgatccctat ccgggaactt gagcaagttg aagtcggcga aaggcaccat gacgtactcg
    43801 gcctgcccgc cggtccagcc gcccatgtcc acatacccgt aggcagagcc cgcgcgagcc
    43861 gggtttacgt tcaggcagac gcctgtcttg cgctctttgc agttcctgca gcgcccgcag
    43921 gcgatgttga agggcaccga gcagatatcg ccttcgtcga tgaactggac gtcgttgccc
    43981 ttctcgatga cctcgcccgt gatctcatgc cccagagtct gcccgacggg ggccgtcgtc
    44041 cgcccgcgca ccatgtgctg gtcggagcca cagatgttgg tggcgacgat cctaaggatg
    44101 acggcgtgcg gggctgcctt ggtcataccc atcgcatcgg cgacctcttc tgggacctcg
    44161 agcttcgggt actcgatgtc ctctaccgct acctcgcctg gccctttgta gaccactacc
    44221 ctgttgtcag ccattagctg aatctctcct ttcccgttag ccggagacga tcaaattcaa
    44281 taattcctat tcccactcgc ccgcgccgcc aaactttcct ttgccgttgc aggagcgcat
    44341 ctcagatatc caggctagct cctttccccc gaaacccact ccggacaaag taccagatgt
    44401 agcgtgtcgg tgcaagaacg agactacata tacaggtggt tggtacggtt gcgcgacggg
    44461 tgggagcata gtttcgtgag ggaatgaata aggttagaat caggggagag ttctgacaac
    44521 gacattcttg cctagaaggg gccacgcatg ctcttcaagc tcacggagaa agatggtctt
    44581 tggaagaaga tccggaatca tgtcggcacg acggtcgagc accccgtaag cccggcgacc
    44641 cgtgacatcc gctcgcgcat cttcgatgag ggagtagaaa agaccgtcag cgtctgtccc
    44701 tactgcgcgg taggctgctc gactctcgtc taccatcggg acggacgtat catagatata
    44761 gagggaaatc ctgatagccc cataaacgcc ggtaccctct gtcccaaggg gtctgcggcc
    44821 ttcgggctgc acgtctcgcc ttaccgttgg acgaaggtcc gctaccgcag gccgtattcg
    44881 gcggagtggg aggacctcag cttagaagag gcgctcgcca tgatcgccga tagggtcaag
    44941 aacacgcgcg acgaaaactg gcaggacacc gacgagaggg gtaagaagct cagccgctcg
    45001 acggcggtcg cctcgatcgg cggggccacg atagacaacg aagagaacta cctcataacc
    45061 aagctcttcc actctttggg cttcgtgcgc attactaacc aggcgcggat atgacacagc
    45121 tccacggtgc ccggtctggg catcacgtac ggtaggggtg gggccaccac taccctgcag
    45181 gacctgcaga attccgactg catcttgatc gaaggctcca acatggcaga ggcccacccg
    45241 gtaggcttcc ggcacccgat gatcgccaag aagaggggcg cgaagctcat ccatgtcgac
    45301 ccgcgcttca cgcgcacctc tgcgctgtgc gacatctacg ccccacttag accgggcacg
    45361 gacatagctt tcctgggcgg cctgatcaac tacgttatcc agaacgagcg gtacttcatg
    45421 gactacgtcg tccactacac caacgccgcg accctcgtca ccgaggagtt cgaagaggcg
    45481 ggcatctcgg gcctgttctc tggctgggat gaggaaagta ggcgatacga cccgtcgagc
    45541 tggcgctacg agggcacgcc catagactac gccgccgtcg gggggagcca gggccgcctt
    45601 atcggcgaag gcgtcggcgg ggagtcggcg ccgaacgagg acgagccgcg cccgaccgat
    45661 ccgacgctcg agcacgagcg ctgcgtcttc caactcctca agcgccactt cgccaaatac
    45721 acgccggaga tggtcgagaa ggtctgcggc acacctcagg aagctttcct cgaggtcgcc
    45781 gagaccatct gcgagaactc gacgcccgag aagacgacct cgctctgcta cgccgttggc
    45841 tggacccagc actccaaggg cgtccagatc atccgcaccg ccgccatcct gcagctcctc
    45901 ctcggcaaca taggacgtcc cggcggcggg atcatggccc tgcgcgggca cgcgacgatc
    45961 cagggctcga cggacatagc gaccctctac aacatccttc ccggctacct gccgatgccc
    46021 gacgtgaaca tggacaacga cacctggagc gactacgtgg agagccagac ggcttccacg
    46081 gggtggtgga acaacttccc caaatacata accagcctgt tcaaggcctg gtacggcgag
    46141 gccttcgacc ccgcgagggg cgacgaggtc ttcgaccact tcccgcgcct gtccggtgac
    46201 tactcctact acccgacgat cgtcgacatg aaggacgggg acgtgaaggg ctgcttcgtc
    46261 ttcgggcaga acttcgcggt cggcgggccg cacgtcaagc tggcccagga tgccctgagg
    46321 agcctggact ggctcgtggt cctcgacgcc tacgagatag agacagccac cacctggaag
    46381 atggaaggcg taaaccccga ggagtgcgca atggaggtct tcttcatccc ctctgctctc
    46441 gtcgccgaga aggacggctc cttcacccag acccagcgca tgttgcagtg gcacgacaag
    46501 gccgtcgaac cgccgggcga tgccaggagc gacgcctggt tcgtcttcta tctggggcgc
    46561 aggctcaagg aactctacga ggactccgaa gagccgcgcg acacgctcat aaaggctatg
    46621 acctgggact atccgaccga gggcgaacgc gaggagccac acatagagag catcctcaaa
    46681 gagatcagcg gctacaccgt ggccgacggc cggccggtcg gcggcttcgc cgagcttgaa
    46741 gacgacggca cgacggcctg cggcggttgg atctattcgg gcgtctactc aggcggcgtc
    46801 aaccaggcca gaaggcgcaa gccccagacg gatcagcccc cggcggcgaa cgagtggggc
    46861 tgggcctggc ccgcagaccg tcgcatcctc tacaaccggg cctccgccga ccccgagggc
    46921 agaccctggt ccgaagagaa aaagtacgtc tggtgggacg agcagcaggg cgagtgggtc
    46981 ggccaggaca tccccgactt cacggcgacc aagcggccgg attacaggcc cgagcctgat
    47041 gccaggggcg acgacgccat acccggagac ggacctttca tcgtcaacgg cgacgggctc
    47101 ggctggctct tcgcccccgt gggcctggcc gacggtcccc tcccggtcca ctacgagccc
    47161 gtcgagtcgc cggtcccgaa cctgctctac gacaacgtcc agtacgaccc ggcggtaagg
    47221 ctcatggaca ggccggacaa cccgtacaac ccgcccgagg acccgaggtt cccgtacgtc
    47281 gtaaccacct accgcctgac cgagcatcac accagcgggg cgatgagccg ttggatcccc
    47341 tggctctccg agctgcagcc cgagttgttc tgcgagatca gccccgagct ggccgccgag
    47401 aagggcgtga acaataccgg ctgggtgacc atctccacga gccgcggcag gatacattgc
    47461 aaggccctgg tctccgaccg catcccggtc tacgacttcc agggaaagaa gcttcacact
    47521 atcggcctcc cgtaccactg gggtcccagc ggcctggtca aaggcgacgt ggtcaacgac
    47581 ctgatcccgg tcgccctcga agcgaacgtc gccatccacg aagccaaagc cttcacggcc
    47641 aacctcgaag cgggccgggt gtaggggcat ggccgcaaga gacagggccc tgacaggagt
    47701 cctcagcgga ctgggagcca cgctcgtgct gaccgggctg cgccaggcct ggtcaaaggt
    47761 agggctggtc ttcgagacag ccccgatgca ggtcgtggac cgcgtcgagg aactcggtct
    47821 cctcggtgaa ctctcgccgt ggggccggcg ccttctgacc gtagcagcgc acttcgcata
    47881 cggcgccggg acggggatgt ccttcggcct cctgcgcagg gagaagggcg gacccgggga
    47941 agaagcctcc gtggggtcgg cgctcggggt gctggcctgg ggcgtgggat gggcgagctg
    48001 gctgccgcta accggcgtcc acaaggcgcc ctggacccag aagacgccga aagtcctgct
    48061 ccccgtaata gaccacgcgg tgttcggcgc ggcctgggga ctcctctact gggcgctccg
    48121 taaggcgcgc gcgtgagtgt cggggaacgc cgcagcaggg agatgatgca gcaggatctc
    48181 atggacctcg gggtctggct ctcgaagcat cccgaggcgg ccggcaagaa cctcaagccc
    48241 ataaggagcg cggaggctct cgagccgggc gtctactaca acatgggcac ggggatggtc
    48301 gacaggatct tcacccccca gcgcgtcgcc ctcgggcaca agatgttccg cataagcaaa
    48361 gatccggcat cgtcggtcga ggaggtaagg cggaaggcga tggaagggag gggccggtgg
    48421 cgacggggtt cctgaccgat acgacggtct gtataggatg caaggcctgc gaagttgcct
    48481 gcaagcagtg gaaccagctc cccgccgacg gctacgagct gacgggcgac tcctatgaca
    48541 acacaaagca actctcgggc accacctggc gccacgtcgc cttcatagag aacccgaaga
    48601 agcgccgctt gaccggagat gtggacctcg tagcgctcgc cgaggatgtg acggaaccgc
    48661 tgcccggcgg ggccgagctc gacgccctgg gacgctgggc tttcatgagc gacgtgtgca
    48721 agcactgcgt cgaggcgccg tgccagcaag cgtgcccgac aggcgccatc atccgcaacg
    48781 agttcgacaa cgtctacatc cagcccgaca tctgcaacgg ctgcggctac tgtgtccccg
    48841 cctgtcctta cggcgtcata acccaggtcc cgaacgatgg ccgcgccttc aaatgcaccc
    48901 tctgctacga ccgccaacgc gacggcctcg aaccggcctg cgccaaggcg tgcccgacca
    48961 actccatcat tttcggtgag gtcgaggacc tgaaggctat cgccgaagag cgggtacagg
    49021 agcttcacaa caagggctac agccaggcct acgtctgggg cacgcctgag gtagggggga
    49081 caggcgggat cgggggcaac cactcgaagt tcatcctcat ggacgaaccc gagacctaca
    49141 accttccccg tcatccctac ctgccgcagg agaaggtcaa gggaggaacc atagccagta
    49201 tggtcgctgg cgcagtactc ggggcggtgg tggcgctggc gttcagggac tagggggaga
    49261 taggtgagcg atacgacgca ggacggcgtc cggcagagca acgggaaggt cgagacctcg
    49321 caccatcccg agacggatcc tgggcgcggg gacaagaatt actacggcat cccgcccata
    49381 aagcacgccc actggacctg gcagatcccg gtgtacttct ggatcggcgg gataggagca
    49441 ggcgtccact tgttctccac cattacccag atcctcggtc acaaagacga agcgctcacc
    49501 cgcgccagcc gctacacggt gctcggcacc atggtgctga gcccgatact tttgatctgg
    49561 gacctcggac gtcccgagcg cttctacaac atgctccgca tcctcaagct ccgctccccg
    49621 atgtccaccc agagctggtc ggtcttcgcc ttcggtaacc ttagcggcct gatcgccgcc
    49681 cgccaggccg ccgacgatgg actgctcgga gaagataacc cactggtgcg cctggtaaag
    49741 ctcatccctg cgcgcgctct ctccatagtg gcactgcctg tcgggctcaa cgtcgggtcc
    49801 aataccggaa acctgatctc cgccacctcg gtccccctgt gggcgcgcaa ctggctgttc
    49861 atgggaccta cgttcctgac ctcaggactc tccacagccc tctcgtggct ctcgctcgtc
    49921 ctccacctga cgcactcggg tgagaagaag accctgcgca cactccaccg cgccgagaag
    49981 gctaccatcc tcatagaggc agggctcata gccgcctccc tggtgaggat gagccggtgg
    50041 agcaaaccac tcttctccag agaggtcgcc ccgctcttct tgggcggtac cctcctcggc
    50101 ggcatagtcg cgccaatggt cctgctcttc ggcaaagaga gccgttccag gagcatcctc
    50161 gcttccgtgc tcgcgctcgc tggtggcctc gccttccgct tcgccataat taaggggggc
    50221 cgcatcagcg ccgacgaccc cgaagcctac ttcaccttcg ccagcggcga gagtgccccg
    50281 cagcccgagg acagcatcta gcgagagagt tactcgcccg catactcctg atctgtgacg
    50341 ggctccatcc agtcagtgcc gccgtcggtc gtggtggcga tattgatagc caagtggacc
    50401 atgtaggtct cgggggtggc accgtgccag tgcctctcgt ccggggcgaa atatacgacg
    50461 ccaccggcct cgacctcgac gggtggctcg tcctcgctcg cagcgcggca ggtgcctgtg
    50521 actatgtaga ggatctggcc ttccgggtgg gtatgccagt gcgtacgggc gccaggctcg
    50581 aagagcacgc ggttgacggc cacgtcgggg gaagtgccgg taggtgcggc gtctctccat
    50641 accgtgccgg tgaaccagtc ctcgggccct ggtccggtct gcatatcttt caacgtgacg
    50701 actttcatgg ggtttcctcc cttcggtcgg aagcatttca gcacgcctgc ttcgcaggct
    50761 tgtcagcact tcagcatgtc agccttccca aggcctggct gaaatgctga aagcggagcc
    50821 cgaagggcgc agcgtgctga caagcggagg cgaagccgga gcgtgctgaa gtgctttttc
    50881 atgccaatcg atgctcaagg atggggaaca aatgcggttc gtcgttgttc tcggggatgg
    50941 tcttctccac cttcaggagg gctttgcggc cccggatctc gaggctggag atgtggttgt
    51001 ggaaccaggg cgcttcgtgc agcagttgcc agcggaagtc cgattcctcg agaccggcca
    51061 gtcgggagag ggcttcgctg atgcgcctgc cccgttcggt ccacccaaag cgcatggcga
    51121 ggcgttcggg tagacccagc gggtttcgca ggggggagct tgtcgactgg tagacggggg
    51181 ttttgatgtc gccgaaatcc atcttcgcca ggtagccgtg gtgcacgtcg ccggagagga
    51241 ccgttaccga ggcgggaggg tcgccgttcg aatgctcgcc cgctgcgaca gagcgtagca
    51301 ggtatgttag ttcggtgaag gacttgttga aggcggacca gtgctccagg tcgacgagct
    51361 ggcggaccga ctcaccgatc ctggccgcaa ggcggcccca ggcgccggcg cagacggcct
    51421 cgctggccgc ttccaggtgg tggagtccgg ggccgaggag gaaagggagc gaggtgccga
    51481 agaggaggtg gtcgaagtct cccgtggcca tctcgacgat ccaggcccac tcctcgtcgt
    51541 cgagcatgga gcggtggccc tccttaagta cccgccctgc ccgcgagtcc atcatgatga
    51601 gacggaccct gccgaagtcg cggtggtagc tccagcgcgt acccgctatc tcgcggtcgg
    51661 ccttgtaggc aaactcgcgc aagacgcggg tggggttctc ggccttctgc accttctcga
    51721 agagcgcgtc ctcctccagc tctcgcgggg agaggttgcc gaggtgctgg tagacccagt
    51781 aggacatgaa agcacctgtg atgcgatcct cccaccatgg cgtggcgcgc atctggcgga
    51841 cccaggcttc cgaaatgttc cagtcgtcgt tgacgtcgtg gtcgtcgaag atcatcgccg
    51901 ttggcacggt agagaggagc caccttatga cggggtccct ccaggagtcc cagtaaaggc
    51961 gcgtgtactc ttcgaagtcg gcgacctgct cgcctggcgg ctcgttcgtg tcgcgccgcg
    52021 agcggatgaa atcgagggcg ccctcggaga cctcgtcggc gtagatctga tcccccaata
    52081 gtagcagcgc gtcaggccac ctctcgtggg gctcatgcat catgcgcagg gccagagcct
    52141 ccagggcatc ccgctcgagg ccgtctctgt gcgcatccga agcaagcgtg tacgggattt
    52201 tatgcggcac cgagacgcgg caggatccgt aggataggtt cagcactcca gcaggcgcga
    52261 tggtcctgat aacagacggc ggaaagtcgt gtgctccgtc gggccagacc ttctcgccgt
    52321 cgaggaagac ctcgtactcg taggtctttc ctggctcgag gtctgtgatc ctcaccagag
    52381 tgtagtggtg gccctcgatc tcgaaggtgc tcgcgctgtg gccgagaacc tccacctcgc
    52441 aggccgcgtc ggtctcgacc cagactgtgg cgtcggtctc tcccgtgtag cgtagcagcg
    52501 gaccgagaac gaggcccggc gcgggtctca cgccaggccg acgaggcggg ccatcacggt
    52561 tatgccgaac gtgatcagca cgacgccgat gatgtcgaag acgataccgg agcggatcat
    52621 cttggttatg gggatcatgc ccgagccgta gacgatggcg ttcgggggcg tggagaccgg
    52681 cagcatgaag ccgtaagagg caccgaagac ggcggcgagg gccgggatca ccgggttcac
    52741 accggccgag gcggcgatcg ggagcacgat ggggaccacg atcgtggcgc tcgcggtgtt
    52801 ggaggtcgtc tcggagatga tcagggcgac gatcgtcgag agaagcgtga tggcgaagag
    52861 gcttgacacg ccgagcccct cggtcagccc cgtgcctatc gtctccgcga gaccggtctc
    52921 cgagaggagg gtcccgagcg cgatgccgga gccgaacagc aagatagtgc cccagtctat
    52981 ccttaccgcc tcgttccagt tgagggtgaa gcgccgttcg gaccagttga tcggcagtat
    53041 gaacaagagt cctgcggcca caaccgcgac caccccttcg ttgatgcggt cgtagaccaa
    53101 cccatacgcc gttgattcgt cgccgaagat gattcctatt atgcccggca ggatccagaa
    53161 gaacactgcg aggccgaaga cgatcagggt gttgcgttcc ccgcgggaga gaccgcctag
    53221 cttctcgcgc tcctcggcga tgaactcctc ggcgccggag atctggcggg tctccggcct
    53281 gttgagcagg atcaagatga tgcacagcaa gacgaacatg gtcagtacga tggggaacgc
    53341 cagcaccacc cattcgaaga acgagatcgt cgtgcctgtc tcctgctcga tgaactcgcg
    53401 tccgatcagg ttcggcggac tcccgatggg ggtcagcaag cctccgacgc tggcaccgta
    53461 ggcgatcatg agcataagcg cggtgccgaa gcgcaacctc ctgacgtcgg tgtctccctc
    53521 ggactggtca ctgaccagcc ctgccagagc ccccatgatt cccagaccta tggggaaaag
    53581 catcgcggtg gtggccgtgt tggacacgaa cccggagatc gaagccgcga tcaggccgaa
    53641 accgacgatc acgccgtagg tcgagcgcgc gatgccgggg atggagagca ccaggtaggc
    53701 gaacctcctg tcgaggccat gggtcatcat cgcctgggct atgatgaagc tgccgatgaa
    53761 caggaagatt gtgtcgtcgg cgaaggaacc gaagatgacg tccgccgggt cgtcctcggc
    53821 gtccgtggct acctgcggaa cgttgaggat cacggccagg gccagcccga taacggcggt
    53881 cacggggata gggatggcct ccgtaaccca gtagacgacg acgaaggcga gaacggccgc
    53941 cagggactgc tgggtgtagg gaagcccgag cggcaacagc atcattatga gaagcaccgg
    54001 tggcccgagg aagagcccga tcgtccgtcg tcttctctcg aacctctcct ccgccggcga
    54061 cagtacctct cgctgctcgt ctagcgtctt gtaggtgccg cccggtctaa tgtcctcgct
    54121 caagaaaccc tccctctcct caccacttct cctggcctcg aagtatagcg actgatgtaa
    54181 cgcgttaccc gcatgtttag cctcgtactg tgccctcgat cagatagtcc actacccctt
    54241 ctagcgagcc gcttcttttg tgagcggccc gctgcaactc cgcccctgtg ccgagctcca
    54301 cgatctccag tacgccgcac agttcctcct cgcatccgag gtctcttgcc acgggccgca
    54361 gctcctcgac cagcccacgg gccatcgaac gggctggcat cgccctgccc gcgacgaagt
    54421 cgtagaaatc ggcgtccatc ccgtagcgca cggcgcgcca tttgttctct gcgttgtaca
    54481 tagcgtcctc cgcaggccgc ttctccacac cggctctcgc tacgaggcac tggacgagcg
    54541 cggcgagggc taccgtgtag gatgggtccg tctgtacgtc cggggcgcgc aactccacgg
    54601 tgccgaactt ctggtgcgga cgcgcatccc accagcacca ggagtagtcg gggatgctcc
    54661 ccgaggcgac gagggcctcg atgtggtcct cgaagtcctc ccagttccgg aaggttggag
    54721 gtaagccaga tcggggaaac atctcgaaga tcttgatccg ggtcgtggcc agcttagtgt
    54781 cgaacccgcg ccagaagggc gagttggccg agagcgccac gaggagcggt acgtatctgg
    54841 cgagcgtgtt gacggccagg atcgcagcgt ccccgtcggg gacggcgacg tggacgtgca
    54901 gcccgtagat agcctgcgtc ctcgcaaccc agcccatctc ctcttcgacg cgccggttat
    54961 gatccccacc cgtgagcggc tgttctgtag gttcggagaa aggatgagcg cccgccgcca
    55021 ctatctcgac cccgctgctg cgcgcgatct cgcgcacctc ccggcgcaac ccaggcaact
    55081 ctcgcgcgag gtcggcggcc gacgcgaaga tgggggagag catctccaca caagaccgcg
    55141 agagctccga ggtaatcagc cgcgaaccat cccccgcggc gagcacctcc tccaccgccg
    55201 gggtcaaccc gcgggaggcc gggtcgacca gctgaaactc ctcctcgacc ccgaccgtat
    55261 agggcgccgt cgcgccgaat ccaccatcca tccgcgtact ctagcaagtc agcacgcgcc
    55321 ctgtcgggcg cttgtcagca cttcagcatg tcagccaaaa ccaaaaagct gaaatgctga
    55381 aagcgaagcc tgcaagggcg aagcgtgctg acaaggcggc gcagccgcca tgctgacaag
    55441 cgaaggcgtt agcctgagcg tgctgacttg ctgacctgcg cgggtttagg aaggctgggc
    55501 cttcctaaac ccgctacgac tgagggctag ctggatgcgg tgggagcttt cgaggtcgcg
    55561 gacggcgtcg aagagtttgc cttcggggac ggtggcgtgc cagcgctccg tcttgtagcc
    55621 gtagcgacgc tttaactcgc gcaccgagtc gcccgtgtag cgcagggcga agatgagctt
    55681 gcgggcctcg tcggtcctgc gcggtaggag gttgtgacgc ccctcggtcc accaggagat
    55741 cccgggcatc ggttcgcttg agcgtaggat caactgcgtc tcccactcga ccctgagggc
    55801 gcccatctcg aacgtaccgg gcagcacgct caacggtccc agggagaatc cgtcgtctct
    55861 gtggaatccg aaggtgcgtc cgagtcgttc ccttagcttc gcgccgggca gccgctcgta
    55921 acgggagcgc aaggcgagca g
//
LOCUS       DATMVF010000217         9094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_574684, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000217 DATMVF010000000
VERSION     DATMVF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574684"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1501
                     /locus_tag="VN178_11230"
     CDS             167..1501
                     /locus_tag="VN178_11230"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HWS81587.1"
                     /translation="MSSTTKTAPIPLSRKPRGPVEPGAKTACIRTFGCQMNVHDSDRI
                     RRMIQDAGYAEVQRYEDADLVILNTCYVRENAVDRVRGHLGELNRLRREGRVKRVALT
                     GCIGAAEESAALRQTYDIDLVLGTHNTYELAEFVGLPTMRETYTPELPGTEGGHSAFV
                     TIMTGCNYQCSYCIVPTVRGRMICRPMDSVIGEVENLVAGGTGYITLLGQTVDAWRHE
                     GKRFCDLLEAVSESAPRVWFTTSHPSNMEDRTLRAIGERDSIVKRLHLPVQSGSDRML
                     KVMHRGYKAERYRRKIEIFREAVPDGTLSTDIIVGHPGETEEDHEQTLRLVEDCAFDS
                     AYIFKFSPRQGTEAASLPGTVPDSIVQRRFLEVLRQIEQNAFSRNQHKVGGEEEIYIR
                     HGRTEDGKAIGETWSGHAVHVKTDAAPGEYVRATIESAGPHVLYAGEVDTSV"
     gene            1646..2377
                     /locus_tag="VN178_11235"
     CDS             1646..2377
                     /locus_tag="VN178_11235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HWS81588.1"
                     /translation="MQVYRELPVITNQARRRPAQLVGVISVTEDWTVARHRALAEEII
                     AKEADFVLDAGTGMYLNAILLDIPLAPKVPAELRRRAEEAVGGEPNPRRAARARELEL
                     IGAAGRGSIWDGDLSYDTSIVYVRPEKHDLDAAIEERSRSIALNGLEEAGRLREMTAA
                     GTRISPQVMGSVGVRELLGHLAGTISVQQAQELIATRTRRLARRQMRWFDKLVRTLEG
                     RARITVVQGTEHRLDSHNMHDILGI"
     gene            2374..3618
                     /gene="hflX"
                     /locus_tag="VN178_11240"
     CDS             2374..3618
                     /gene="hflX"
                     /locus_tag="VN178_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWS81589.1"
                     /translation="MSEERAVLAGAGERRHMAELGRLASTLGIEVAGVLEQGRRDGTG
                     YLGRGKREELRGLVEGAGARFVIADDELSASQARVLERACGVSVVDRTELIIRIFEAH
                     ARDAASRLEVELADLEYRLPRVKGKNPELSRLGGGGVTTRGPGEQQLEYDRRVIRERI
                     ATINRKLQEEKAGRAVRGARLREEGPPTVALVGYTNAGKTTILNALSGARRSTKDRLF
                     ETLETTTRLVEGSRKNGPYGMWPDFVVTDTVGFIRKLPTQLVHSFASTLEVARDSDVT
                     VVCADASSEELEEEIQTVTRSLYGRNEDDGVGTGPMVMCLNKMDLVSQTRAAELRRDH
                     PHAVAISAIRDSGALLGEIYRAIASRRERMRVFIPHAEYAAASRLYGLAEIHAQENTE
                     SGLWLDVSLPRSASGRYASYRV"
     gene            3615..4382
                     /locus_tag="VN178_11245"
     CDS             3615..4382
                     /locus_tag="VN178_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HWS81590.1"
                     /translation="MKIFRLYPPPAREVFAVYEDLVLPPAGRREGARPYVITNTVSSV
                     DGRTTMGGKAAGIGSRTDRRTMRTLRSKVDAVMIGAGTLRAEKLSLGLDGPDGLPQPL
                     AIIVTDSGEVPLEEHLIVEEGQEVLILLSDAAPGRVVERLRALAPVVKAPANSTGAVD
                     LQAALRILEAERGVESLLVEGGPALNHALFSRNLVDELFLTVAPELLGGSAAGTLTLL
                     RGPEIPACGRPTLELVSVHLAAAELFLRYSLTRTAPS"
     gene            complement(4395..6491)
                     /locus_tag="VN178_11250"
     CDS             complement(4395..6491)
                     /locus_tag="VN178_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HWS81591.1"
                     /translation="MDLDLRVAEEAAKRWWDRQQERKARRTAIDAGRISEIESPERIK
                     KRVDRLTEATLKRKVERSVAGSDRGPSLLLTETIGLERVLGKSDFLGTDFLEMALAVS
                     RFVGRINIRSRPGRPVGFGTGFMVSPRLLLTNNHVLQTGRDALYSEVEFDYQYDRYGR
                     LLPVVAYGLEPETFFITSKELDFTLVAVRELSLSGVDLKTYGWSPLIGAQGKALLGDS
                     LNIIQHPRGEAKQIVLRSNQLLDLFDDFAHYVTDTEPGSSGSPVYNDEWEVVALHHSG
                     VPKMQDGNYIAKDGSIWRRGMDPGDLEWVANEGIRVSSLLDHVKKARLTGGQGRLREE
                     MLNGEPPNPLEAAAMAKEDTPPAHGGQGSFTWTIPLHVTVSLGTSSPAISAATDAQGG
                     TIAEPSDRPPTTVGEEAISIDPNYGNRKGYDPTFLGDGAKSIPLPNLSDLMKVDVAVN
                     TQAVDPDEYVLPYNHFSVVMNKRRKLAFFTAVNIDGNLSFPIRRDTDKWFVDPRIGED
                     EQTGEAVYANNPLDRGHLVRRLDPAWADSEALAKVANDDTFHFTNCSPQHENFNQNRT
                     TWAGLEDYILDNAIAHDLRVSVFTGPVLSPQDPQYRGVQLPRQYWKVVAMVKDDGALS
                     ATAYLLSQESLLGGLEEFSFADYKTFQVPVRRVEELTGLDFGDLKEADPADVLELAER
                     EALGAREIVTLEDLTL"
     gene            7208..8704
                     /locus_tag="VN178_11255"
     CDS             7208..8704
                     /locus_tag="VN178_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWS81592.1"
                     /translation="MIAAASLGSGMAFLDSTVMNVALPAVQAELGASAREVQWSYGAF
                     ALVLSALILLGGTLGDRFGRRRLFVIGAAIFGAASMWCAFVPDTGQLVVARAVQGLGG
                     ALLIPASLAIVGASFEGKQRARAIGAWGALSGLAMAVGPVLGGWLVEYVSWRAAFLMA
                     PAMAIVAIPIALSHVPESRDPEAHRLDLAGAILATGGLAGVVYALIESSTRGFGDSRV
                     LAALVVGLVALSGFFVVEQHARYPMVPPSLFHSRSFTGANLVTLLFYMALTGSLFFLP
                     FLMMQVHGYSAFVAGSVFLPFVAMALLIGRFSGRISARFGAKVPLMVASLALAAGLLL
                     FALPGAEHGSYWTAFFPAMVVQGFGMALAITPLTTVALGSVEGEHSGMASGVNNAVTR
                     VAGLLAVAVLGTFIYGAYSASLDARMEDMDMPGEIRIAMEAAKADLGAAEAPASVNDG
                     MEAQIEHAIDESFVAGFRAVMVVSAALALASALIAALMVGDPRTPSTR"
     gene            8856..>9094
                     /locus_tag="VN178_11260"
     CDS             8856..>9094
                     /locus_tag="VN178_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81593.1"
                     /translation="MTEQKPHGLDGHASDPDGPRQLPYSTYPRTADVADHGLQPLPES
                     LPLVDFAQAKPYRSIRYTTVLATELTKEQLLQMAWV"
BASE COUNT         1686 a         2733 c         3017 g         1658 t
ORIGIN      
        1 gatggggggc ctgtgttgca acttggggag gaggaaagtg cgcgcattga ggatatactc
       61 aggcgggcgg cgctggccgg gtacctggag gtcgaagggt tgagggtcga gagccgtccg
      121 ggggagatct ccctcagggc cagagtctac gagccggagg aacagtatga gtagcacgac
      181 gaagacggcg cccataccgc tctccaggaa gcctcgaggg ccggtcgaac ctggtgccaa
      241 gaccgcctgc atcaggacct tcgggtgcca gatgaacgtg cacgactcgg accgcataag
      301 gcggatgatc caggacgccg gctatgcgga ggtccagcgc tacgaggatg cggacctcgt
      361 aatactgaac acctgctacg tccgcgagaa cgccgtggac cgcgtcaggg ggcacctcgg
      421 agagctaaac cgcctccgcc gcgaggggcg ggtaaagcgg gtggccctca cgggctgcat
      481 cggcgcggcc gaggaatcgg cggccctcag gcagacctac gatatcgacc tcgttttggg
      541 aacccataac acttacgagc tggcggagtt cgtgggacta ccgacgatgc gggagacgta
      601 cacgcccgaa ctgccgggca cggaaggtgg acacagcgcc ttcgtcacga tcatgacggg
      661 gtgcaactac cagtgcagtt actgcatagt cccgaccgtg agggggcgta tgatctgccg
      721 cccgatggac agtgtgatag gggaggtgga gaacctcgtc gccggcggta ccggctacat
      781 tacgctcctg ggccagacgg tggatgcgtg gagacacgaa ggaaagaggt tctgcgatct
      841 actggaggcc gtctccgaga gcgcgccgcg ggtatggttc accacgagcc acccttccaa
      901 catggaggac cgcactctga gggccatcgg ggagcgggac tccatcgtga agcggctcca
      961 tctgcccgtg caatccgggt cggacaggat gctcaaggtc atgcaccgcg gctacaaagc
     1021 cgagaggtac aggcgtaaga tcgaaatctt cagagaggcg gtgcccgacg gcacgctcag
     1081 cacggatatc atcgtgggcc accccggcga gaccgaggaa gatcacgaac agacgctcag
     1141 gctcgtggag gactgcgctt tcgactcggc ctacatcttc aagttctccc cgagacaggg
     1201 caccgaggcc gccagtctac ccgggaccgt gcccgattcg atcgtccagc ggcgcttcct
     1261 cgaggtgctg agacagatcg agcaaaatgc atttagtagg aatcagcaca aagtcggggg
     1321 cgaagaggag atctacatcc gtcacggcag gacggaggac ggcaaggcca tcggggagac
     1381 gtggagcggt cacgccgttc acgtaaagac agacgctgcg ccgggagaat acgtgagggc
     1441 cacaatagag tccgcgggac cccacgtgct gtacgcgggc gaagttgaca cttcggtctg
     1501 agggaccacg gccaaggata gcgagaagcg ggcgctcgta gtctgcgggc cgacggccgg
     1561 cggcaagagc gaactctccg acgcccttgc tgagaggctc accgaggcag agctccggtg
     1621 cgtacccaca ctggccgtag attccatgca ggtctacagg gagttgcccg tgataacgaa
     1681 ccaggcccgc aggcggcctg cgcagctggt cggcgtcatc tccgtgaccg aggactggac
     1741 cgtagccaga cacagggcgc tcgccgagga gatcatcgca aaggaggccg acttcgtcct
     1801 cgacgcggga acggggatgt atttgaatgc gatcctgctg gatatccccc tcgccccgaa
     1861 ggtccctgca gagttgcgcc gccgggccga ggaggcggtc gggggtgagc ccaacccccg
     1921 cagggccgcg cgggccagag aactggaact catcggggcc gcaggccggg gttctatctg
     1981 ggatggagac cttagctacg acacctccat agtctatgtg cgccccgaga agcatgacct
     2041 cgacgccgcc atcgaagaga ggtcgaggag cattgccctg aacggcctag aggaggccgg
     2101 aaggctgaga gagatgaccg cggccgggac gcgtataagc ccgcaggtaa tgggctcagt
     2161 gggagtgcgc gagctcttgg gccacctcgc ggggacgatc tcagtgcagc aggcgcagga
     2221 gcttatagcg accaggacaa gaaggctcgc ccggcgccag atgcgatggt tcgacaagtt
     2281 ggtccgtacg ctggaaggtc gcgccagaat aaccgtagtg cagggcacgg agcaccgatt
     2341 agattcgcat aatatgcatg atatactcgg catatgagtg aggagcgtgc ggtactcgcg
     2401 ggagctgggg agaggcgcca catggcggag ttggggcggt tggcgagtac ccttggcatc
     2461 gaggtcgccg gagtactcga gcagggccgc agggacggca ccggttacct cgggcgtggc
     2521 aagcgcgagg agttgcgggg attggtagaa ggtgcggggg cgcgattcgt tatcgcggac
     2581 gacgagttga gtgcgtccca ggccagggta ctggagaggg cgtgtggcgt ctccgtcgtg
     2641 gacaggacgg agttgatcat ccggatcttc gaggcgcatg ccagggacgc ggcgagccgc
     2701 ctggaggtcg aactcgccga cctggagtat cgtttgccga gggtcaaggg caagaacccc
     2761 gagttgagcc gcctgggggg cggaggggtt accacgaggg gccctggcga gcaacagctg
     2821 gagtacgaca ggcgcgtgat ccgcgagcgc atagcgacca taaaccgcaa gctccaggag
     2881 gagaaggcgg gtagggccgt acggggtgcg cggctgaggg aggaaggacc accgaccgtc
     2941 gcgctggtgg gctacacgaa cgcgggcaag accaccattt tgaacgccct aagcggggcg
     3001 aggaggtcga ccaaagacag gctcttcgag acgctcgaga cgaccacccg tctcgtggag
     3061 gggagcagga aaaacggccc ttatggaatg tggcccgact tcgtggtcac cgacaccgtg
     3121 ggattcataa ggaagctgcc cacgcaactc gtgcactcct tcgcctcgac gctcgaagtg
     3181 gcgcgggact ccgacgtcac ggtggtctgc gcggacgcct ccagtgagga actggaggag
     3241 gagatccaga cggtcacgcg cagtctctac gggaggaacg aggatgacgg ggttggcacg
     3301 gggccgatgg tgatgtgcct caacaagatg gacctcgttt cacagacgcg cgccgccgag
     3361 ctcaggcggg atcacccgca cgcggtcgcc ataagcgcga tccgggacag cggggcgctc
     3421 ctaggggaga tctaccgcgc catcgcgtcg aggagggaga ggatgcgagt ctttatcccc
     3481 catgccgagt atgcggccgc gagtcgcctc tacggcctgg cagagataca cgcccaggag
     3541 aacaccgagt cgggcctgtg gttggacgtc agcctgcccc gctcggcctc gggcaggtac
     3601 gcctcctaca gggtgtgaag atattcaggc tctaccctcc ccccgcgcgg gaggtctttg
     3661 ccgtctacga agacctggtc ctgccgcccg cggggcgtcg cgagggcgca agaccgtatg
     3721 tgatcaccaa cacggtcagt tcggtggatg gcaggaccac catggggggg aaggccgccg
     3781 gaatagggag taggaccgat cgccggacga tgcggaccct ccgttcgaag gtcgacgccg
     3841 tgatgatcgg ggccggtacg ctgcgcgccg agaagctctc ccttggcctc gacggcccgg
     3901 acggtctgcc acaaccgctc gcgatcatcg tcaccgactc gggcgaagtt ccgctggagg
     3961 aacaccttat cgtcgaggaa ggccaggaag ttttgatcct gctctccgat gcggctcccg
     4021 ggagggttgt cgaacggttg cgcgccctcg cgccggtggt gaaggcgcct gcgaactcaa
     4081 ccggtgccgt cgacctccag gcagccttgc ggatcctcga agcggagcgt ggtgtcgagt
     4141 ccctcttggt cgagggcggg cccgctctca accacgccct cttctcgcgc aatctcgtcg
     4201 acgagctctt ccttaccgtg gcgccagaac tgctcggcgg aagcgccgca gggaccctta
     4261 ccttgctcag aggacccgag atcccggcct gtggccggcc cacccttgaa ctagtctccg
     4321 ttcacctggc ggctgctgag ttgttcttgc gctactcgtt gacccgaacc gcgccaagct
     4381 agccggggct atcgtcagag cgtgaggtct tcgagggtca caatctcgcg cgcgcccaaa
     4441 gcttcccgct ccgcgagttc caaaacgtct gcggggtctg cctccttcag gtcgccgaaa
     4501 tcgagaccgg tgagctcttc gaccctgcgg acagggactt ggaaggtctt gtagtcggcg
     4561 aacgagaatt cttccagccc cccgagcaag ctttcctggc tcaagagata ggccgtcgca
     4621 gagagcgccc cgtcgtcttt caccatggcg acgaccttcc aatattgcct cggtagttgc
     4681 actcctcgat actgcggatc ttggggactc agcacggggc ccgtgaacac cgagacgcgt
     4741 agatcgtggg cgatggcatt gtcgaggatg tagtcttcca aaccggccca ggttgttcgg
     4801 ttttgattga agttttcgtg ttgaggggag caatttgtga agtggaaagt atcgtcgttg
     4861 gcgactttgg ccagtgcttc tgaatcggcc caggcagggt ccaacctgcg aaccaggtgg
     4921 ccccggtcta agggattgtt ggcgtacacc gcctcgccag tttgctcgtc ttcgccgatc
     4981 cgcggatcca caaaccactt gtcggtatcc ctcctgatgg gaaagctgag gttgccgtcg
     5041 atgttgactg cggtgaagaa tgccagcttg cgcctcttgt tcattaccac actgaaatgg
     5101 ttgtaaggaa ggacgtactc gtccggatcg accgcttgcg tgttcacggc aacatcaacc
     5161 ttcataaggt cggaaagatt cggcaaaggg atgctcttgg cgccatcgcc caagaaggtc
     5221 gggtcataac ccttacgatt gccgtagttc gggtcgatgc tgatcgcttc ctcgcccacc
     5281 gtggtagggg gtcggtctga gggctccgcg attgtgccgc cctgcgcgtc ggtcgccgca
     5341 ctgatcgcag gcgaggatgt cccgaggcta acggtgacat ggagcggaat ggtccatgtg
     5401 aaagaacctt gcccgccgtg ggccggcggc gtatcctctt tcgccatcgc cgcggcctcc
     5461 agggggttag gaggctcgcc gttgagcatc tcctccctga gcctcccctg tccccccgta
     5521 agcctggcct tcttgacgtg gtcgaggagg ctgctcaccc gtatgccctc gttggccacc
     5581 cattccaggt cgcccgggtc cataccccgc cgccagatcg aaccgtcttt ggcgatgtag
     5641 ttgccgtcct gcatcttggg gacccccgag tggtgcaatg ccaccacttc ccactcgtcg
     5701 ttgtagaccg gcgagccgga agagccgggt tcggtgtcgg tcacatagtg ggcgaaatcg
     5761 tcgaacaggt ccagaagctg attggaacgc agcacgatct gcttcgcctc tcctctgggg
     5821 tgctggatga tgttgagcga atcgccgagc agcgccttgc cctgcgcccc tatcaagggg
     5881 ctccagccgt aggtcttcaa gtcgacgccg cttagcgaca actctcgcac cgccacaagg
     5941 gtgaaatcga gttccttgct cgttataaag aatgtttcgg gttcgagacc gtaggctacc
     6001 accggcaaca ggcggccata ccggtcgtat tgatagtcga actccacctc gctgtataac
     6061 gcgtctcgac cggtctgcaa cacgtggttg ttcgtcagca gcagacgcgg cgaaaccatg
     6121 aagcccgtgc cgaacccgac tggccgccca ggccgcgaac ggatgttgat ccgaccgacg
     6181 aagcgagaga ccgcgagggc catctccaga aaatccgtgc cgagaaaatc ggacttccca
     6241 aggacccgct ccaggccgat cgtctccgtc aacaggaggg acggccctcg gtcgctgcca
     6301 gcaaccgagc gctcgacctt gcgcttcagg gttgcctcgg tcagtcgatc tacccgcttt
     6361 ttgatccgct ccggcgattc tatctcggag attcttcccg cgtctatagc cgtacgcctc
     6421 gctttccgct cctgttggcg atcccaccag cgtttcgcgg cctcctcagc cactcggaga
     6481 tctaggtcca tgtcgtcctc caactcgtcg tgtccacaat ccatcgaatg taagcagacg
     6541 gcgatgaata ccggatgaat ccgcacgaaa atccctgccg tcagcatccg agaacccccc
     6601 ttcttctcgg agtattggtc cgaacgcctg gacacgacgg tggtaagttc ggttctggac
     6661 cggagtgagg taattgcccg ggcttagcga agggttgtgt ttcctccctg atccggcggg
     6721 gtcaggaact tcgggcggag gtagccggta gccggtttgc tccctacggc gttcgcgtcg
     6781 tcttcgacga ggccggcatc gtcgacgcct cgtgcggttg tccttatgac tggggtggcc
     6841 tgtgtaagca catcgtctcc accctgctcg cctccctgca ggtgcccgag tcggcaagcg
     6901 cctttagatc ggatgttgga tcttcgcgcg atccccgcac ctacgggtat actgcttaca
     6961 ggtgatagcg ctgagagcgc cagggcagtc gtagacgcgt accctggagg tgatcgaaag
     7021 taggaggcaa tacctcgttg ggacgtgagg tggcgaacga gcgtcaggtc ttacagcttc
     7081 gggcaaagcg gccgataaga cgctcctgga gaacgagata gtgcccgccg tactcccggc
     7141 atgcgaaata ggtgttgtcc agtccaccaa gccatccgcg ctgtgccggc cagattcgcg
     7201 gccgtgggtg atagcggcag cctctctggg ctcgggaatg gcattcctcg acagcacggt
     7261 aatgaacgtt gccctacctg cggtgcaggc cgagctcggg gcgagcgctc gggaggtgca
     7321 gtggagctac ggggcattcg ccctggtgct ttcggcgctc attctcctgg gaggcaccct
     7381 gggagaccgc ttcggaaggc ggcgcctctt cgtcattggc gcggcgatct tcggtgcggc
     7441 ctcgatgtgg tgcgcctttg tccccgatac cggccaactc gtcgtggcca gggcggtgca
     7501 ggggctggga ggggcgctgt tgattcctgc ttcgctggcg atcgtcgggg cttctttcga
     7561 agggaagcaa agggcgaggg ccatcggtgc ctggggggcg ctcagcggcc tggcgatggc
     7621 ggtagggccg gtcctgggcg gttggctggt cgaatacgtc tcctggcggg cagcattctt
     7681 gatggcgcct gccatggcta tcgtggcgat cccgatcgcg ctctcgcacg tgccggagag
     7741 ccgtgacccc gaggcccaca ggctcgacct cgccggtgca atcctcgcca ctggcgggct
     7801 cgctggcgtg gtctacgcac ttatcgagtc ctcgaccagg ggctttggcg actctagggt
     7861 gctcgccgcc ctcgttgtgg gattggtcgc cctttcgggc ttcttcgtgg tcgaacagca
     7921 tgccaggtat ccgatggtac caccttcact gttccactcc aggagcttca cgggcgcaaa
     7981 tttggtgacg ctcttgttct acatggcctt gaccggctca cttttcttct tgcccttcct
     8041 catgatgcag gttcacgggt actcggcctt cgtcgcgggc agcgtgttct tgccctttgt
     8101 ggccatggcc ctactgatcg gccgcttctc cgggcgcatc tctgcccgct tcggggcgaa
     8161 ggtgccgctc atggtagcct ccctcgccct cgctgcgggt ctgctgcttt tcgccctgcc
     8221 gggcgccgag cacggctcat actggacggc gttcttccct gcgatggtcg tgcagggctt
     8281 cgggatggcg ttggcgataa ccccactgac gacggtagcg ctaggctcgg tcgagggtga
     8341 gcactctggg atggcctcgg gcgtgaacaa cgccgtaacc agggtggctg gcctcctggc
     8401 ggtcgccgtt ctgggcacat tcatctacgg ggcgtactcc gcgagcctcg acgcccgcat
     8461 ggaagacatg gatatgcccg gcgagatccg gatcgcgatg gaagcggcga aagcggatct
     8521 cggggcggca gaggcgccag ccagcgtgaa tgatgggatg gaagcacaga tcgaacacgc
     8581 catagacgag tccttcgtcg cagggttcag ggccgtgatg gttgtctccg ccgccctcgc
     8641 gctcgccagc gcactgatcg cggcgcttat ggtcggggat ccgaggacac cctccacaag
     8701 atgagaacct gcctgccgga tgtacgggtc aagcaccggc cggactgcac actagcgcat
     8761 gatatctcca gatggcatca tggcggctcg ggagaaccgt ttcggagtgg gaaccccgca
     8821 agtcgattac cgaagaagag caagggagta acagcgtgac cgaacagaag ccacacggtc
     8881 tcgacgggca cgccagcgac cctgacggac cgcgccagct gccctacagc acttatccga
     8941 ggaccgcaga tgtcgccgac cacgggttac agcccctgcc tgagtccctg ccgctcgtgg
     9001 atttcgcgca ggcgaagccc taccgcagca tccggtacac cacggtcctc gcgacggaac
     9061 tgacgaagga gcaactcttg cagatggcgt gggt
//
LOCUS       DATMVF010000218         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_308131, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000218 DATMVF010000000
VERSION     DATMVF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308131"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VN178_11265"
     CDS             complement(<1..321)
                     /locus_tag="VN178_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81594.1"
                     /translation="MRIFRDIMKRILGFKLLRRSTSVLILVLADGLALFFGLGVAAWG
                     GGSDPAAGLAPLLLAAWITLFSAFRLYDKAPVRRSPGGLLGAAFCWAGLVTIGTTIYP
                     GSDLG"
     gene            433..1182
                     /locus_tag="VN178_11270"
     CDS             433..1182
                     /locus_tag="VN178_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81595.1"
                     /translation="MERFGSYVDEHPAIVRLEKWLQIYAGKHPSAFYGLYRLSRKDRA
                     RVVTPDTQLVIEGFPRSANTFARVAFNSAQSGRVRIAHGLHVPAQVIRAAQWRIPTLV
                     LIRRPKDAVLSFAIRDPISVDQALRYYLSFYETVEEYRDAYVLALFEEVTEDFGGVIR
                     RINDRFGTTFLPFSHDERNVDGVLARIEENSRKRFGEASLGNKVSRPFASREKLKREV
                     GYELEDPKRRDLISRAETVYERLTRKPAQGH"
     gene            complement(1179..1994)
                     /locus_tag="VN178_11275"
     CDS             complement(1179..1994)
                     /locus_tag="VN178_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWS81596.1"
                     /translation="MAPHVGPRAGRIDVLGVGVDPVTVDELHAEIARLVRAGKPGLVL
                     NVNAHCLNLCYEDAALRGSLNGADVVFCDGAGVMLAARILGGRIPARITYADWIWRLA
                     DFAAAGGFSLYFLGARPGVAQEAARRLRERYPGLRIVGVRHGYFDHSAGSAENEAILE
                     EINATAPDILLVGLGMPLQEIWLTQNRDRLIAGVALSGGAVFDYASRRVRRGPRLLTE
                     HGFEWLARLLSEPRRLWRRYLLGNPLFLLRVLRQLLSRRTGRDPVRGLQQDRR"
     gene            complement(1979..3103)
                     /locus_tag="VN178_11280"
     CDS             complement(1979..3103)
                     /locus_tag="VN178_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81597.1"
                     /translation="MRKPPVVILSGIRWDFLWQRHQTLATLFAGAGYPTVFVETTGLA
                     NPRPSGDSLRRVFGRIRRSGGAGEKQPAERDLTVYAPLTAPPTYGAFRWSNAKFLVPR
                     IIRDLRKIAGPHPVVVAYPPTRTTLGLISGLDPKLVLYDCADDYEHFPGAPKDIKETE
                     RELLLRADLVSCTSAHLLEKARRIRPDAFLSGPAVDYEQFAPLQGPAGEVRTVCFFGH
                     LSLERIDFDALRTIAGAGCGLRIVGAVGGVEMGFFEVPGIDYRGEASHASLPSALEGV
                     DAFVLPYKVNALTRSISPAKTYECLATGKPVVAAHLPAMRDLAGHVYLAQRPEDYVYV
                     LRNLQHLETEEKRRARIELARENSWDARFSELEETLWRHM"
     gene            complement(3115..4026)
                     /locus_tag="VN178_11285"
     CDS             complement(3115..4026)
                     /locus_tag="VN178_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS81598.1"
                     /translation="MMGRPLSRLPGGVYVDLEKGGHRSSVFLAGSGRSGTTWLSEIIN
                     HRRGYRYVFEPFNPREVGPFGHFRTKQYLRPDDRREVFLGPARLALTGRLRDPWTDRF
                     NGRIVARRRLIKDIRANLLLGWMRAHFPGMPIILLLRHPCAVVASRLDLGWKDNLSET
                     MEQKELVEDYLLPMEAQIRAAKDAFERHLFLWCIDNYVPLEQLGPQDVHLAFYENLLA
                     RPQAELTSLFSFLGEDFDDRLYRRLGRPSPLSRKNTPRPSPEGWRTRVSARQLERTME
                     ILGLFGLDRIYGEGTMPEPSFALTDGV"
     gene            complement(4159..5805)
                     /locus_tag="VN178_11290"
     CDS             complement(4159..5805)
                     /locus_tag="VN178_11290"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWS81599.1"
                     /translation="MREVRPPGNASEERFSGMEHKGERDKYRRPGRLLTVFKGLVMLV
                     LLAATAYGMLDTGLYRDALWLPVAAGILALLFVTLFVRGFYWDLPAVGILLVGLLAAL
                     VAVKGLSMVWTISETETIKETLRSSMYLAAFLMALAALTSGRQVGSLVDASVFMVAAV
                     AGYGLLQKISPIEYPVRSLDGVRMDSTLGYANTTAVILGMGVVLALARMSTLRSFVLR
                     GLYAAVTLSLFIALYLTVSRGGIGSLGIGLIILFVITSGRLRMFADLLLLSLPGAWLW
                     WQMQSLPGLLGTGVSRQQKIADGTLFRNDLILALVVAFVLQAAYTIVMNRYEPSPVLR
                     RRLGILVVAGAVLAVTLGAFVVVERYGGPGQAYSTFLGSANETANAGQRLASLDISSR
                     GDYWRVAWAAWKERPLTGTGAGTFQYTWLEDRPGLKGVKQVHNVYLEQGTETGLFAFV
                     ALLGFVAVLVGYTGRAVWRSGPQGDRRLLLAGLGSALVVYLVSSAFEWHWYIPASTLF
                     FFILAAVAVKFASREDWGAAEAPGADSEAKLPRTPVEAEG"
     gene            complement(5843..6475)
                     /locus_tag="VN178_11295"
     CDS             complement(5843..6475)
                     /locus_tag="VN178_11295"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HWS81600.1"
                     /translation="MRLGAISDGGARGLLDPDSPVRRHYEELADNLLRVRGTSSVVVT
                     SPEPGTGRTSICLGLGAALAGMGRRAAIVDCNLEDSQLHRMLGEPNFVGLTSGLDDSK
                     PLEHFGYEVIPGLLVVPTGPVLVDPASLLEDEGIPRAVRGLQEGRDLVLLDAPVAARV
                     LRSPNLLGGFDGVLLVVHASRTPKGVARETTDDLLEAGANLLGVVLNGCS"
     gene            complement(6477..>6704)
                     /locus_tag="VN178_11300"
     CDS             complement(6477..>6704)
                     /locus_tag="VN178_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81601.1"
                     /translation="AGAVVEQKALPGDYSPRPLIYAAIAAGVGLLIGGAAALILEGRA
                     SGWRGVRDAELTLRVPVLGAIPDYSSKKTGG"
BASE COUNT         1267 a         2202 c         2148 g         1087 t
ORIGIN      
        1 tccgaggtcg ctcccggggt agatcgtcgt ccctatcgtt accaacccgg cccagcagaa
       61 tgcggccccg aggagtcccc cggggctgcg cctgacgggc gccttgtcgt agaggcggaa
      121 agcgctgaag agcgtgatcc aggccgcgag caacagcggc gcgaggccgg cggccggatc
      181 agatccaccg ccccaggcgg ccactcccag cccgaaaaag agcgccagcc cgtccgcgag
      241 caccaggatg aggacgctcg tagagcgcct cagcagcttg aagccaagga tcctcttcat
      301 tatgtctcta aagatccgca cggggtcctc cgtgcgagga gtataccgtc gcgcaaggcc
      361 gaccggtccc cgcagagtgt gtaagattga agtgagatag aaggcaacga ggcggagggc
      421 acaggagtga gagtggagcg gttcgggtcc tacgtggacg aacaccccgc catagtcagg
      481 ctcgagaagt ggctccagat ctacgctggc aaacaccctt cggctttcta cggtctgtac
      541 cggctatcgc gaaaggaccg ggcgcgcgtc gtgaccccgg acacacaact ggtcattgag
      601 ggattcccga ggtcggcaaa cacgtttgcc agggtggcct tcaacagtgc gcaaagcggg
      661 cgggtgcgca tcgcccacgg cctacacgtt cctgcgcagg tgatccgggc cgcacagtgg
      721 cggataccca ccctggttct gattcgaaga cccaaagacg ccgtgttgtc tttcgccata
      781 cgagacccga tctccgtcga ccaggccctc aggtactacc tctccttcta cgagaccgta
      841 gaagagtacc gcgacgccta cgtactcgca ctcttcgaag aggtcaccga agacttcgga
      901 ggggtcatcc ggcgcataaa cgaccggttc ggaacgacct tcttaccgtt cagtcacgac
      961 gaacggaacg tcgacggcgt gctcgcccgc atcgaggaaa attccaggaa gaggttcggg
     1021 gaggcgtccc tgggaaacaa ggtctcccgc cccttcgcca gcagagagaa gctcaagcgc
     1081 gaagtgggat acgaactgga ggacccgaaa cgtagggatt tgatctccag ggccgagacc
     1141 gtttacgagc gcctgacacg caagccggct caaggtcact aacgacgatc ctgctgaagg
     1201 ccccttacgg ggtctctccc cgtcctcctc gagaggagct gtctcaggac ccgcaacagg
     1261 aacagggggt tgccgaggag gtagcgtcgc cacaggcggc gcggctcgct aagaagccgc
     1321 gccagccact cgaagccgtg ctcggtcagc agccgcgggc cgcggcgcac cctcctcgag
     1381 gcgtagtcga atacggcgcc gccgctgagg gctacgcccg cgatgagccg gtctctgttc
     1441 tgcgtcagcc agatctcctg tagcggcatc ccgagcccga ccagcaggat gtcgggggct
     1501 gtggcgttga tctcctcgag tatcgcctcg ttctcggcgc tccccgccga atggtcgaag
     1561 taaccgtgac ggacgccaac gattctcaag ccgggataac gctccctcaa ccttctcgcg
     1621 gcctcctgcg cgacgccggg tcgggcgccg aggaagtaga gcgagaagcc cccggcggcg
     1681 gcgaagtcgg ccagtcgcca gatccagtcc gcgtaggtga tgcgtgccgg aatgcgcccg
     1741 ccgagtatcc tcgccgcaag catgaccccg gccccgtcac agaagacgac gtcggcaccg
     1801 ttcagcgagc cccgtagtgc ggcatcctcg tagcagaggt tcaggcagtg ggcgttcacg
     1861 ttcaagacga ggccgggttt gccggcccgt accaggcgtg ctatctccgc gtgcagctca
     1921 tctacagtaa ccgggtccac gcccacgcca agaacgtcga tcctgccagc acgaggccct
     1981 acatgtggcg ccacagggtc tcctccagct ccgaaaagcg agcgtcccac gagttctcgc
     2041 gggcgagttc gatgcgggcg cgtctcttct cctcggtctc gagatgctgc aggttgcgca
     2101 ggacatagac gtagtcctcc ggcctctgtg cgaggtagac gtgcccggcg agatcccgca
     2161 tcgccggcag gtgtgcggcc acgacgggct tgcccgtggc caggcactcg taggtcttgg
     2221 ccggcgagat ggagcgggtg agagcgttga ccttgtacgg cagcacgaaa gcgtcgacac
     2281 cctctagagc cgaaggcaga gaggcgtggg acgcctctcc cctgtaatcg atacctggca
     2341 cttcgaagaa gcccatctct acgccgccca cggcgccgac gatacgcaaa ccgcagccag
     2401 cccctgcgat agtccgcagg gcatcgaagt cgatgcgctc caggctcaag tggccgaaga
     2461 agcacacggt gcgcacctct ccggcgggac cctgcagggg cgcgaactgc tcgtagtcta
     2521 cggcggggcc gctcagaaag gcatcgggcc tgatgcgtct ggccttctct agcaggtgcg
     2581 ccgaggtgca tgagacgagg tcggcgcgca ggaggagctc gcgctccgtc tccttgatgt
     2641 ccttcggagc gccggggaaa tgctcgtagt cgtcagcgca gtcgtagagc acgagcttcg
     2701 ggtcgaggcc cgagatcagg cccagcgtcg tcctcgtcgg gggataggcc actacgacag
     2761 gatgggggcc tgctatcttc ctgaggtcac ggatgatgcg cgggacgagg aacttcgcgt
     2821 tcgaccaccg gaatgctccg taggtcggcg gagcggtgag cggcgcgtaa acggtgagat
     2881 ctctctcggc cggctgcttc tctcccgcgc cgcccgagcg acggattcgc ccgaagaccc
     2941 tgcgcaggct gtctccgctc gggcggggat tggcgaggcc cgtggtctcg acgaagacgg
     3001 tgggatagcc ggcgccggcg aagagggtgg cgagcgtctg gtgcctctgc cagaggaagt
     3061 cccaccttat accggagagt atcaccaccg gcggcttgcg cacaggggag gctcctatac
     3121 tccgtccgtc agggcgaaag agggttctgg catggtccct tcgccgtaga tgcggtcgag
     3181 gccgaagagg cccaggatct ccatcgttct ctccagctgg cgggcgctca cgcgggtacg
     3241 ccacccttcc ggggaaggac ggggcgtatt cttccggctc aggggggaag gccgtcccag
     3301 tctgcggtaa agacggtcgt cgaagtcctc gcccaggaag gagaacaggc tcgttagttc
     3361 tgcttgggga cgagcgagca ggttctcgta gaaggcgagg tgcacatcct gtgggccgag
     3421 ttgctcgagg gggacgtagt tgtctatgca ccagaggaag aggtggcgtt cgaaagcgtc
     3481 tttggcggcc cggatctgcg cctccatggg taggaggtag tcctccacca actccttctg
     3541 ctccatcgtc tccgagaggt tgtccttcca tccgagatcc agcctcgatg ccacgacggc
     3601 gcaggggtgt ctgagcagga gaatgatcgg cattcctggg aagtgcgcgc gcatccagcc
     3661 gaggagcaag ttggccctta tgtccttgat cagacgccgc ctcgcgacga tgcgcccgtt
     3721 gaagcggtcg gtccaggggt ccctgagccg gcccgtgagc gcgaggcggg ccgggccgag
     3781 aaacacctcc ctcctatcgt ctggtctgag gtactgtttg gtcctgaagt gcccgaaagg
     3841 accgacctcc ctggggttga agggttcgaa gacgtagcga tatcccctac ggtggttgat
     3901 gatctccgag agccacgtgg tcccactccg cccgctccct gccagaaaga cggagcttct
     3961 gtggcctcct ttctccaggt cgacgtagac acctcctggc agccgtgaga gaggcctgcc
     4021 catcatcgta ccgccccctg ccccagggtg gcctccgagg catccgcgat gaaggccagg
     4081 aaccggaaga agacagcgag ggacgcgaat atgctgtcgc tctccgatcc gggatagcca
     4141 atgcccatcc ggccgatgct atccctcggc ttcgacgggt gtacggggaa gcttggcctc
     4201 gctgtcggcg ccgggagcct cggcggcgcc ccagtcttcc ctcgaggcga acttcactgc
     4261 gacggcggcg aggataaaga agaatagcgt cgaggccggg atgtaccaat gccactcgaa
     4321 cgccgaggag acgaggtaga ccacgagcgc ggagcccagc ccggccagaa ggagccggcg
     4381 atctccttgc ggacccgacc gccagacggc tcttcccgta tagccgacga ggactgccac
     4441 aaacccgagc agggccacaa aggcgaacaa gccggtctcg gtgccctgct cgagatacac
     4501 gttgtggacc tgtttgactc ccttgagccc cggtctgtcc tccagccacg tgtactggaa
     4561 agtccccgcc cccgtacctg tgaggggacg ctccttccac gccgcccagg cgaccctcca
     4621 gtagtctccc ctggagctta tatccagcga ggccagtctc tggccggcgt ttgctgtctc
     4681 gttcgcgctg cccagaaacg tcgagtaggc ttgccctgga ccgccgtacc tctcgacgac
     4741 cacgaaagcg cccagggtca ccgcaagcac tgctccggcc acgacgagga tcccgagtct
     4801 ccgcctgaga accggtgagg gctcgtagcg gttcatcacg atggtatagg cggcctgcaa
     4861 cacaaaggcc acgacgaggg ccaggatcag atcgttcctg aacagcgtcc cgtcggcgat
     4921 cttctgctgt cgtgagacgc cggtcccgag tagcccgggg aggctctgca tctgccacca
     4981 gagccaggca cccggcaacg agaggaggag aaggtcggcg aacatccgca ggcggccgct
     5041 cgttatgacg aagaggatga tgagtcctat ccccagggac ccgatgccgc cgcgagagac
     5101 cgtaagatag agcgcgataa acaaagagag cgtgacggcg gcgtagagcc ccctcagtac
     5161 gaaactgcgc agagtactca tccgcgccag ggccagcacc acccccatcc ccaggatgac
     5221 ggcggtggta ttggcatacc ccagggtcga gtccatcctt accccatcca gggagcggac
     5281 cgggtactcg atcgggctta tcttctgcaa gagaccgtag cccgcgacgg ccgcgaccat
     5341 gaagaccgag gcgtcgacga gcgagccgac ctggcgcccc gaggtgagcg cggccagcgc
     5401 cataaggaaa gccgccaggt acatagacga gcgcaacgtc tccttgatag tctccgtctc
     5461 acttatcgtc cagaccatgg agaggccctt tacggcgacg agcgcggcga gcagcccgac
     5521 cagcaggatg ccgacggcgg gcaggtccca gtagaagccc cgcacgaaga gcgttacgaa
     5581 cagcagcgcc aggatgcccg ccgcgacggg cagccagagc gcatccctgt agaggccggt
     5641 gtccagcatc ccgtaggccg tcgcggccag gagcaccagc atgacaagac ccttgaaaac
     5701 ggtgagcagc cgacccggac ggcggtactt gtccctctcc cctttgtgtt ccataccgga
     5761 gaagcgctcc tcgctcgcgt tgccgggggg ccggacttcc ctcactccct ggctcccggg
     5821 tgagtctctc ggcgatcgct catcaggaac atccgttcaa caccaccccg agcaggttgg
     5881 cacccgcttc cagaaggtcg tccgtcgtct ctcgggcgac gcccttggga gtccgggagg
     5941 cgtgcacgac gagcaagacc ccgtcaaagc cgccaaggag gttcggggac cgtagcaccc
     6001 gcgctgccac cggcgcgtcg agcagcacga ggtcccggcc ctcctgcaaa cctcgtactg
     6061 ccctcggaat cccctcatcc tccagcagtg aggcggggtc aaccagaacg ggaccggtcg
     6121 ggacgaccag aagtcccggg atgacctcat acccaaagtg ctctaagggt ttgctgtcgt
     6181 cgagaccgct ggtaagcccg acgaagttcg gttcgccgag catcctgtgc aactgcgagt
     6241 cttcgaggtt gcagtccacg atagcggccc gccgtcccat gccggcaagg gcggctccga
     6301 ggccgaggca gatgctggtc cgcccggtgc caggctcggg actggtgacg acgacgctac
     6361 tcgtccccct cactctcagg agattgtccg ccagctcctc atagtgacgc ctcaccggcg
     6421 agtcggggtc gagcaacccc ctggcaccac cgtcgcttat ggccccgagc ctcacgtcaa
     6481 cccccggttt tcttcgaaga gtagtccggt atggctccca gcacgggcac cctcagcgtc
     6541 aattcggcgt cgcgtacgcc ccgccagccg ctggcccgcc cctcgagtat gagagcggcg
     6601 gcgcccccta tcagcaagcc caccccggcg gcgatggccg cgtagatcaa gggcctggga
     6661 ctatagtctc ccggcagtgc cttctgctct acgacggccc ccgc
//
LOCUS       DATMVF010000219         5797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_71373, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000219 DATMVF010000000
VERSION     DATMVF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71373"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..472)
                     /locus_tag="VN178_11305"
     CDS             complement(71..472)
                     /locus_tag="VN178_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81602.1"
                     /translation="MIDTIESGRESDSHRYYLDDMILYYAVSRAYARGAASLGRAKEA
                     IIEKVLLRSNDDGSFGDELSTACAVCSLVDFEYKGITRLRDAARYLEEHQSADGSWRR
                     IAMYCQPGTYYGSEELTTALCLEALTNVAAM"
     gene            727..1278
                     /locus_tag="VN178_11310"
     CDS             727..1278
                     /locus_tag="VN178_11310"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWS81603.1"
                     /translation="MQESGTLPESLWTLAHLFADYGLRMGAGQYEDLLFPSLKNVSIE
                     DSRAMAEHKSGAEYALFATAGATLATEDPSVLEAYASFGSCFGIAQQLINDAQDIWGH
                     KRGRDLSKGKRTLPVVHALSTLGGERRVRLRGLLAAARESRERHDGVRELLAEAESLR
                     YTALIVWLYQQQARSHLARASPR"
     gene            complement(1460..1612)
                     /locus_tag="VN178_11315"
     CDS             complement(1460..1612)
                     /locus_tag="VN178_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81604.1"
                     /translation="MWPAPGSGQDAPLSRLHTLNVLGEEVAGAIGVSGGTVDQEVAEA
                     GVAAFA"
     gene            1670..2779
                     /locus_tag="VN178_11320"
     CDS             1670..2779
                     /locus_tag="VN178_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HWS81605.1"
                     /translation="MEIRAAVAHEANKPLQIETVELEGPKAGEVLVEIKATGVCHTDE
                     FTLSGADPEGLFPAVLGHEGAGVVVAVGPDVRNLEEGDHVIPLYTPECRECKSCLSRK
                     TNLCTAIRSTQGQGVMPDGTSRISLNGEKLYHYMGTSTFATHTVLPEIAVAKIREDAP
                     FDKVCYIGCGVTTGIGAVVNTAKVEPGSNVVIFGLGGIGLNVIQGCRMVGADKIVGVD
                     INPDKKTLAENFGMTHFVNPNEVEEDLVGYLVDLTGGGADYSFECIGNVNVMRQALEC
                     AHRGWGESIIIGVAGSGQEISTRPFQLVTGRVWRGSAFGGARGRTDVPKIVDWYMDGK
                     IEIDPLITHTMPLERINDAFDLMHEGKSIRSVVTF"
     gene            2792..3658
                     /gene="fghA"
                     /locus_tag="VN178_11325"
     CDS             2792..3658
                     /gene="fghA"
                     /locus_tag="VN178_11325"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HWS81606.1"
                     /translation="MAKSRVKTTAANRCFGGVVSFHEHPSQATGSLMRFSVYVPPQAE
                     GGPVPVLYYLAGLTCTEETFQIKAGAQRYAAEHGVMLVSPDTSPRDAGIPGEEEDYDF
                     GTGAGFYLDAELEPYSERYMMYSYVTAELPELLRDAFPGQADTEMQGVFGHSMGGHGA
                     LVLALRNPGLYRSVSAFAPVCAPVLAPWGEKAFSNYLGEDRETWRAYDASELVRERPF
                     ADGREILVDQGTEDEFLDEQLYPHVLEEACDASGQRLTLRRHEGYDHSYYFISTFMGD
                     HILHHARALGAV"
     gene            3676..3870
                     /locus_tag="VN178_11330"
     CDS             3676..3870
                     /locus_tag="VN178_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81607.1"
                     /translation="MPKRGAGFDPPAPRRSARSHYAWRLEAVPVQQLKSAGLDHVQIS
                     TQADEPALSHRIELANTHGG"
     gene            complement(4184..4615)
                     /locus_tag="VN178_11335"
     CDS             complement(4184..4615)
                     /locus_tag="VN178_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81608.1"
                     /translation="MDGRRDDLDCGRPAGLRALGTGKGLLSPEQQSERLDSFVSDVPP
                     LNQPPLKGDLAYGIGLGKDHGWVGHNGEIPGFNTYLFYHPELDAVVVEVNSDISSGKC
                     PEDVSTLNEWPRAIPCELPADRIFEALAEALGKPSPSPPEQ"
     gene            complement(5029..5487)
                     /locus_tag="VN178_11340"
     CDS             complement(5029..5487)
                     /locus_tag="VN178_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81609.1"
                     /translation="MDEADPRAKLEPFQALIGEWSIEMTHPMVEDRVVRGRVTYGWLE
                     GDRFMIQRAVTEHPDFPDSLCVIGVMEGENDLSMQYFDSRGVHRVYAIGFDGRELTLE
                     RDAPGFAQRCSAKLSDDGSTLAGAWRLNENDQGYRDDLAFTYRRVSGGDG"
     gene            complement(5530..>5797)
                     /locus_tag="VN178_11345"
     CDS             complement(5530..>5797)
                     /locus_tag="VN178_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS81610.1"
                     /translation="GDATAQVRKLKEEFDGELQVPGSHRLVQELIESDLVDQINLMVF
                     PVMLGTGKKVFEEKPERRNLRLKESKVVGDGVLVLIYERAADRG"
BASE COUNT         1064 a         1869 c         1791 g         1073 t
ORIGIN      
        1 agacgaatcg gtggggttac tcgttcgtct agagatccct gctgtacgtc tccccggcat
       61 cgctgtgaac ctacattgca gcgacattgg tcaacgcttc gaggcaaagt gcggtggtca
      121 gctcttcgga gccgtagtac gtgcctggtt ggcaatacat cgctatccta cgccatgagc
      181 cgtccgcgct ctggtgctct tcgaggtaac gcgcagcatc cctgagcctc gtgatgccct
      241 tgtactcgaa atcgaccagg ctgcacaccg cgcaagcagt ggacaattca tcgccgaatg
      301 acccatcatc attgctgcgc agcaggactt tctctatgat cgcctccttt gccctgccca
      361 gcgatgcggc gccgcgcgca tacgcgcgtg aaacggcata gtagaggatc atatcgtcga
      421 ggtaatagcg gtgcgagtcg ctctcgcggc cgctctcgat cgtatcgatc aaatacctgc
      481 atgcgtcctt ggtctcgtcg cgatcgccca gatagaacac cacgttggcg ttgacgacgc
      541 tgtctatgtc agacgccggc cctccccgta gcaggggcct gtacgctcct gtacctcggc
      601 gccgacctgt tcgacagcat cctggatcac gagcttccgc cctcgtggca agtccgcgag
      661 ccttcggagg ctagcctggc ggcgaccact cttctcgggg cgctccccca gctttccata
      721 gctcgattgc aagaaagtgg tacgctgcca gagagtctct ggacgctggc ccacctgttc
      781 gcggattatg gtttgaggat gggcgccggc cagtacgaag atctactctt ccccagccta
      841 aagaacgtgt cgatcgagga cagccgggcg atggcggagc acaagagcgg ggcggaatat
      901 gccttgttcg cgaccgcggg agcgaccctg gcgacagaag atccctccgt gctcgaagca
      961 tacgcgtcct tcggttcgtg tttcggcata gcccaacagc tcataaacga cgcgcaggat
     1021 atctggggcc acaagcgcgg tcgagacctc tcgaaaggga agcgcacgct cccggtagtc
     1081 cacgccctat ccaccctggg cggggaaagg cgcgtgcgtc tgcgggggct gctcgcggcc
     1141 gcccgcgaat cccgagagcg ccacgacggg gttcgggaac tcctggcaga ggcggaatcc
     1201 ctgcgctata cggccctgat cgtgtggctc tatcagcagc aagcacggag ccatctggcc
     1261 agagcctctc cccgctaacc tgccgcgcgg gaactctaca agctgctaga tcaagcatcc
     1321 attctgcccc ggcagcggga agcgcaacct tcggactagc ccgcggcaca gttctccccg
     1381 cgttcatcga cggccgtcca ctctccgtgc cttcacctgt agggacgagt ttgaaacccg
     1441 tccctacgag cgaaccgcct tatgcgaagg cggcgacccc cgcctcggcg acctcctggt
     1501 ctactgttcc gccgctcacc cctatcgctc ctgctacctc ctccccgagt acgttgagcg
     1561 tatgtagcct ggagagcggc gcgtcttgcc cgctacccgg tgcgggccac acgcgtgtag
     1621 aatcgcgtgc tagttggcga ttcgcgtcta cgcaacggac aggagggaaa tggagattag
     1681 ggcggcggta gcacacgagg ccaataagcc cctacagata gagacggtgg agttggaggg
     1741 gccgaaagcc ggagaggtac tcgtcgagat caaggcaacc ggcgtgtgcc acaccgacga
     1801 gttcacgctc tcgggagccg atcccgaagg cctgttcccg gcggtgcttg gtcacgaggg
     1861 cgccggggtg gtcgtcgcgg tagggccgga cgtgcgcaat ctagaggaag gcgaccacgt
     1921 catcccgctc tacacgccgg aatgccggga gtgcaagtcc tgcctgtctc ggaagaccaa
     1981 cctctgcacg gcgatacgct ccacccaggg ccagggcgtg atgcccgacg ggacgagcag
     2041 gatctccctg aacggcgaaa agctctacca ctacatgggc acctcgacct tcgccaccca
     2101 caccgtgctg cccgagatcg cggtggccaa gataagagag gacgcgccct tcgacaaggt
     2161 ctgctacata ggatgcggcg tgactaccgg catcggggcg gtggtgaaca cggcgaaggt
     2221 agagcccgga tccaacgtcg ttatcttcgg gctcggcggg atcgggctca acgtcatcca
     2281 gggctgccgc atggtcgggg ccgacaagat agtcggcgtg gacatcaacc cggacaagaa
     2341 gaccctcgcc gagaatttcg gcatgacgca cttcgtcaac ccgaacgagg tcgaggagga
     2401 tctcgtagga tatctcgtgg acctcactgg aggaggcgct gactactcct tcgagtgcat
     2461 cggcaacgtg aacgtcatgc ggcaagcgct ggagtgcgcc cacaggggct ggggtgagtc
     2521 catcatcatc ggcgtcgctg gctcggggca ggagatctcc accaggcctt tccagctcgt
     2581 taccggcagg gtgtggcgcg gcagcgcctt cggtggggcc aggggtcgca ccgacgtgcc
     2641 gaagatcgtg gattggtaca tggacggcaa gatcgagata gaccccctca tcacccacac
     2701 tatgcccctg gagaggataa acgacgcctt cgacctcatg catgagggca agtccatccg
     2761 aagcgtcgta acgttctagc gagaggagag catggcgaag tcaagagtaa agactaccgc
     2821 cgcgaaccgt tgcttcggcg gggtcgtgag cttccacgag catccctcgc aggcgaccgg
     2881 gagcctgatg cgcttctcgg tgtacgtccc gccgcaagct gagggtgggc cggtccctgt
     2941 gctgtattac cttgccgggc tcacatgcac cgaggagacg tttcagataa aggcaggagc
     3001 ccagcgctac gccgccgagc acggcgtgat gcttgtctcc cccgacacca gccctcgcga
     3061 cgcggggata cccggtgagg aggaagacta cgacttcggc accggcgcgg gcttctacct
     3121 cgacgccgag ctcgagccgt actcggaacg ctacatgatg tactcctacg tcacagcgga
     3181 gctacccgag ctattgcgag acgccttccc gggccaggcg gacaccgaga tgcagggcgt
     3241 cttcgggcac tccatgggcg gtcacggcgc actggtgctc gccctcagga atcccgggct
     3301 ttaccgttcc gtctccgcgt tcgctcccgt ctgcgcccct gtcctggcgc cgtggggcga
     3361 gaaggcgttc tccaattatc tcggagaaga ccgcgagacg tggcgtgcct acgacgctag
     3421 cgagctggtc cgcgagcgtc cgttcgcgga cgggcgggag atactcgtgg accagggcac
     3481 cgaggatgag ttcctcgacg aacaactgta tccgcacgtc ttggaggagg cctgcgacgc
     3541 ttcggggcag cggctgacac tcaggcgcca tgagggctac gaccacagct actacttcat
     3601 ctccacgttt atgggggatc acatcctcca tcacgcgcga gccctgggcg cggtctgaca
     3661 agaggcgcta tacgcttgcc caaacgtggt gccgggtttg atccaccggc accgcgaagg
     3721 agcgcccgga gccattatgc ctggcgcctc gaagccgtac cggtacagca gctaaagtcc
     3781 gccggcctcg accacgtgca gatcagcacc caggccgacg agccggccct ctcccaccgg
     3841 atcgaactgg ccaatactca cggcgggtag cagaaggatt gagctcctgc aacccgcgct
     3901 ctcagcacac agctataagc cttcggctct ttgttcttgc tgaaagctta tcgcttatag
     3961 ctcgccgtgc ataaaggttg aaccctcatg cacggcgctg tagggtaacg gagtggatgc
     4021 cggagccttg ccgaagttgt gatcgccgct aggtcgatat cggcggttac cgctgcctga
     4081 ccgactccca ctcccaacgc ccgtgctcgg cggaacaacg gtgccttaga ccggatatgg
     4141 gcctgatgcc gcggccgtac gtacgagaga gacttcgggt cgtctactgc tccggcggcg
     4201 atggcgaggg cttgcccagc gcctcggcga gcgcttcgaa gatccggtct gcgggtaact
     4261 cgcaggggat ggcccgcggc cattcgttca acgtggatac gtcctctggg catttgccgg
     4321 aggagatatc gctattgacc tccaccacca cggcgtccag ttccggatga tagaagaggt
     4381 aggtgttgaa accgggtatc tcaccgttgt gcccgaccca cccgtggtcc ttaccgagcc
     4441 cgatgccata ggccaaatcg cctttcagcg gaggctggtt gaggggaggc acgtcgctga
     4501 cgaacgagtc gagccgctcg gactgctgct cgggtgagag cagacccttt ccggtgccaa
     4561 gcgcccgtag accagcaggt cgtccacagt cgagatcatc tcgccggccg tccatgccga
     4621 ggagggactc cagtcggtag agtcgatggg cttctcgccg gcggaccgtc cttgcagggt
     4681 gtagccctgg gcatgtggct cggggatgga agagtccgct ggatctggga aagacgtgtt
     4741 ctcgaggccg accggtccga tgatctgttc gcggtacaga tcaccaatgg gcttgcccgt
     4801 caccttctct agaaccaaac caagcagcac gtagttcgtg ttggggtaga accactcggt
     4861 acctggttag aacagagggg actccttgat gccgatccgc gccagatctt caggttgcga
     4921 aaccctcgtc gggtcggaca accattcctt cgcccactgc tcgtcctccg tgtagcttgc
     4981 gattccgctg gtcatgtcgg acatctgcct gagcttggct ggctcgcgct agccgtcgcc
     5041 cccggaaacc cgccggtagg tgaacgcgag gtcgtcacgg tatccctggt cgttctcgtt
     5101 cagtcgccag gcgccggcga gggtcgagcc gtcgtcgctg agctttgcgc tgcagcgctg
     5161 agcgaaccct ggggcgtcgc gttccagcgt cagctccctg ccgtcgaacc cgatcgcgta
     5221 gacccgatgg acgccgcgcg agtcgaagta ctgcatcgag aggtcgttct cgccctccat
     5281 cacgccgatc acgcagagcg agtcgggaaa gtcggggtgc tcggtgaccg cacgctggat
     5341 catgaaccgg tcaccttcga gccacccgta ggtgacccgc ccgcgaacca ccctgtcttc
     5401 gaccatcggg tgggtcatct cgatcgacca ctcgccgatc agcgcctgga acggctccag
     5461 cttcgccctg gggtcggcct cgtccatagc actccttcgt tcgcgttttc gagggacacg
     5521 gccctgtcgc tacccgcgat cggcggcccg ctcgtagatc agcacgagca ccccgtcgcc
     5581 gacgaccttc gactccttca gcctcaggtt gcgtcgctcg ggcttctcct cgaagacctt
     5641 cttgccagtc ccgagcatca ccgggaagac catcaggttg atctggtcga cgaggtcgct
     5701 ctcgatcagc tcctgcacca gccggtggct ccccggcact tgcagctcgc cgtcgaactc
     5761 ctccttcagc ttgcgcacct gggccgtcgc gtcgccg
//
LOCUS       DATMVF010000220        14612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_251941, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000220 DATMVF010000000
VERSION     DATMVF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14612
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251941"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VN178_11350"
     CDS             <1..1059
                     /locus_tag="VN178_11350"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWS81611.1"
                     /translation="EVLAEEVGALEERGLEVRGRLLVDGRAHLIMPYHIALDSHREIA
                     LGDARIGTTNRGIGPAYEDKVARSGIRVQDIFDEGILKTKLKAALREKNAIFENVYGE
                     EHYEVADVASWLLSFSEFIGPMVADTGTLLREALDNRERVLLEGGQATLLDNDHGTYP
                     FVTSSNPTVGGAVVGSGIPARHLEYVVGVTKAYTTRVGDGPMPTELFDETGETIRSVG
                     REYGTTTGRSRRVGWLDLPAIKWAASLNGITHVAITLLDVLSAVDEINICVGYEIDGE
                     RVEGYPMHQTDLHHAKPVYKALPGWGEDITWCRMRGDLPEAAQDFVGFVEAEVGAPVC
                     MISVGPERDQAIVERIGI"
     gene            1072..2346
                     /gene="purD"
                     /locus_tag="VN178_11355"
     CDS             1072..2346
                     /gene="purD"
                     /locus_tag="VN178_11355"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWS81612.1"
                     /translation="MKVLVVGSGGREHALVETLAASPLEPEMYAAPGNVGISRIAETV
                     DIPVDDLVSLRDYSAENSIDLTVVGPETPLIGGISEAFWGGGLKIFGPSRAAARIEGS
                     KVFAKELMRHAGVPTARFEAFDREEAALAYLSAHHDYPIVVKADGAAAGKGVTVSRSQ
                     DEAEEAVRASFAGKFGAAGERIVIEEYLEGREASIFVVTDGQDILPFLPAQDYKRIYD
                     GDEGPNTGGMGSYSPIMWMEPATYAAILEDIIRPTIHQLSLIGAPYTGLLYAGVMVTE
                     TGPKALEFNCRFGDPETQVILPRLGSDLLDLMLAAGNEDLAGREIVWSADKAVCVVLA
                     SEGYPETSSSGDEISGLENIPAGVYVYHAATEERDGSFYTAGGRVLNVVGTGPTIIEA
                     RARAYAAVEQIHFEGMHYRTDIALEAIELEEL"
     gene            2343..2774
                     /gene="purE"
                     /locus_tag="VN178_11360"
     CDS             2343..2774
                     /gene="purE"
                     /locus_tag="VN178_11360"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWS81613.1"
                     /translation="MSPSVGILVGSESDLPVLEKCTARLEGYGIEYELEVRSAHRNPE
                     GVSEYASTARERGLKVIICAAGMAAHLAGAVAARTNLPVIGIPVASGSLGGFDALLAT
                     VQMPSGVPVATVAVNGAANAAVLAAQILALSDPELAERLDG"
     gene            2869..4182
                     /gene="purB"
                     /locus_tag="VN178_11365"
     CDS             2869..4182
                     /gene="purB"
                     /locus_tag="VN178_11365"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWS81614.1"
                     /translation="MIERYTLPEIGAVWTEEAKYRAWLEVELAVSRARARLGEIPAEE
                     VEELAEKADFTVERIHEIEWETNHDVIAFVTNVAEYAGDVARHFHFGLTSSDVLDTAG
                     AVQLKEALDLILDEARALTLLLAGMSLEHRDTVMVGRTHGVHAEPTVLGHKLAVWAFE
                     MERNLDRLAHAREVAAVGKISGAVGTYANVDPKVEAITCQDLGLASSPASTQVVQRDR
                     HAEVLSTLAILGSTMEKIALEIRGSQRTEVRELAEPFGRGQKGSSAMPHKRNPILAER
                     LCGMARLLRANAAVGFENNALWQERDISHSSAERVVLPDSTILAFYMLRTTSRILRGL
                     EVHEERMLEKLNSGGGIVFSGRVLLALVESGMNRDDAYRVVQDAAMRAWDGEAGFREL
                     LEADDDVRDRLGDDLDTLFDPAYALRNLGAVFGRVEKLKRRLEDD"
     gene            4175..4900
                     /gene="purC"
                     /locus_tag="VN178_11370"
     CDS             4175..4900
                     /gene="purC"
                     /locus_tag="VN178_11370"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWS81615.1"
                     /translation="MTKELLYEGKAKKVFTTDDDNVLLHRYKDEATAFNAQKRGSWKD
                     KGKTNATMSAAIFAYLDTHGVPTHFVEQVDDTTIKTRKLDMLPVEIVVRNVAAGSLSR
                     LLGFEEGRKLKAPIVELCYKDDDLGDPLLNWYHFRELGVSDEDLEFCEELGLKVNEVL
                     APFFDERGIVLVDFKIEAGRDAEGNLMLADEISPDTCRFWDKETGEKLDKDRFRNDLG
                     GVEDAYAEMLRRVADITQRGAVD"
     gene            5072..5308
                     /gene="purS"
                     /locus_tag="VN178_11375"
     CDS             5072..5308
                     /gene="purS"
                     /locus_tag="VN178_11375"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWS81616.1"
                     /translation="MAESLKIRVLVRPKAGILDPQGEAVKNALPALGFEGVRTVHVGR
                     LVEMEVESTEEVDAMCRRLLSNPTIEEYEWEVVR"
     gene            5337..5999
                     /gene="purQ"
                     /locus_tag="VN178_11380"
     CDS             5337..5999
                     /gene="purQ"
                     /locus_tag="VN178_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWS81617.1"
                     /translation="MRIGVTVFPGSNCDRDALYGVERMGAEAVELWHADADLKGVDAV
                     IVPGGFSYGDYLRPGAIARFANVMGPLEEFAKSGGPVLGVCNGFQVLTEAHLLPGALL
                     RNTHMRFVCDRVRVRVESVDTPWTAGCEPGEELTLPVAHNEGNYFADPRTIVELENEG
                     RVVLRYLENPNGSANDIAGVCNEDRNVVGIMPHPERVCDSALGSDEGLRILRSVLVGA
                     GV"
     gene            6002..8221
                     /gene="purL"
                     /locus_tag="VN178_11385"
     CDS             6002..8221
                     /gene="purL"
                     /locus_tag="VN178_11385"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWS81618.1"
                     /translation="MQETYSSLGLSDFEYDRILELMGRAPNFLELSLFSVMWSEHCGY
                     KNSRPLLGRFPNEGKRVLQGPGENAGVVEVGDGWALAFKMESHNHPSAVEPYEGAATG
                     VGGIIRDILAMGARPVALLDSLRFGPLDEERNRYLFREVVRGIGGYGNAVGVPTIGGE
                     VEFGRAYSGNPLVNAMCIGLIRVEDLVRSSATGTGNLVVLLGSKTGRDGIHGATFASD
                     ELTDESESKRSNVQVGDPFCEKMLIECCLKLLDEDLLVSLQDLGAAGITSSASEMAAR
                     GGVGIEIETDMVPLRESGMEPTEIMISESQERMLAVVEPEKIDEVLWIAERYELVGAV
                     VGRVAEHGDLRVIHDGEVAGTVPAEHLADAPVYERDVVRPWYLDDVPELDLDEIPEPE
                     DYKAVLLQMLGHPNLCSRRPIFTQYDHQVGTDSVVLPGADAAVMRIKGTDRGFAATTD
                     GRGRHCYLDPRGGGAATVAEAYRNLSCVGAEPVAVTDCLNFGSPKKPDGYYQLAECIE
                     GMSEACEAFGTPVVSGNVSLYNETDKGAIYPTPTVGMVGILEDVERHASPGFKREGDL
                     VVVIGGGSPASLDGSEYLEIIHGRVAGRPPRPDLGPGKETADLVREMVRSGLVDTAHD
                     ISGGGEIFAVAEMALAGGLGIEYEEDGLERIRAGRGRTDVALFGEGSGAFVVAVPEER
                     WGEVQDALVGFAYNVVGTTGGERFEIGDLIDIELSDLREAYERDLFESYAPEGGHIG"
     gene            8245..9705
                     /gene="purF"
                     /locus_tag="VN178_11390"
     CDS             8245..9705
                     /gene="purF"
                     /locus_tag="VN178_11390"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWS81619.1"
                     /translation="MGALDVDYRDSEGKPKEACGVFGLYSRELSGELAQRTYFGLYAL
                     QHRGQESAGIAVSDGERTTAMRDMGLVSQVFDGPRLAALEAGHLALGHVRYSTTGSAS
                     WENAQPEFGGRGDVNVAVAHNGNLVNAERLRDGLAEDGFRFYSTSDTTYIAAAAVREL
                     ERGLSVGEAVRGAMRHFEGAYSVAMIFRDKLVAFRDPHGFRPLCIGEVEGGYAVSSET
                     CGLDIIGATFLRDVEPGEVIVIDDDGLTSYTAESPRRALCVFEYVYFARPDSRFDGRE
                     VAGSRLRMGELLADEAPAEADVVIPVPDSGMMAAIGYSQRSGIPYAEGLIKNRYVGRT
                     FIQPTDGMRQLGIRLKLNPLPSVIEDNRVVVVDDSIVRGNTSRKLVELLFEAGAAEVH
                     FRVSSPPIKGPCYYGIDMDSPDQLVGANLTVEEIRAQIGATSLAYLSVDAMIAATQQR
                     KDRLCRACFDGDYPTTGTSSKFALEDRTQYAATRGG"
     gene            9762..10778
                     /gene="purM"
                     /locus_tag="VN178_11395"
     CDS             9762..10778
                     /gene="purM"
                     /locus_tag="VN178_11395"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWS81620.1"
                     /translation="MPENSYEGAGVSIERGEQSVALLRPAVEKTHGPEVLGATGGFAG
                     LYALAGYRDPVLASTIDGVGTKVLVAREVGRYYSLGADIVNHCANDVLATGARPLFFL
                     DYVASGRLDPEAVAGIVSGAAESCGRLGVALIGGETAEMPDVYGRGDFEIVGVCVGAC
                     ERGEVVSGERVRPGDTILGLASSGLHTNGYTLARKVIEDAGVTYDDVPEGLDRSVGEV
                     YLEPHRAYVREVDALRSAVEIRGMAHVTGGGLVGNLPRALGGLGARFDVGSWSEPAVF
                     GLIRTLGGIPEGEMRRVYNLGVGFCAVVSREEAEAGLQALLGTGCDAWRIGEVTEAGG
                     VEFA"
     gene            10771..11352
                     /gene="purN"
                     /locus_tag="VN178_11400"
     CDS             10771..11352
                     /gene="purN"
                     /locus_tag="VN178_11400"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWS81621.1"
                     /translation="MPERLPEVSRFAVLASGSGTNLQALLDAYPENLVVVAGDNKEAF
                     AFQRARRAGVPVEHVDPEGFVGKEDYDRELADRIEAHDVGLVVGAGYMRILSPLFLER
                     FPAVLNVHPSLLPEFRGLRAVERALEAGVEETGVTVHFMVEEVDAGPVVAQQRVPVLP
                     GDNKETLLERLHPVEHGLLVRAVANYFLGRVEV"
     gene            11349..12908
                     /gene="purH"
                     /locus_tag="VN178_11405"
     CDS             11349..12908
                     /gene="purH"
                     /locus_tag="VN178_11405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWS81622.1"
                     /translation="MKRRALISVSDKRGIEDFARRLSGMGFEIISTGGTAKVLSEAEV
                     MVIPVSEVTGTPEILGGRVKTLHPKIHGGILADLTDPDHVKQLVEQDIGPIDLVCINL
                     YPFEETVAGGAPEKEAIEQIDIGGPAMLRAAAKNFGSVTVVPSPEYYEEVLAALQSEG
                     QVPEETRRRLALAAFRRTAEYDGKISGWLGRRVEGESESSDFEAEGFPVRRTVDYERV
                     SALRYGENPHQAAAYYAEAGADHLLSGVEKLQGREISFNNLYDLDAARTLLADLTDLG
                     AQAAVIVKHANPCGAAVADSLEDAYRKALASDPQSAFGGIVALNGEVDGNLASEISSI
                     FTEILVAPGFTQEAREIFSAKPNVRVLVAGPLVRPALSSRHVTGGVLLQNSDAIEDDS
                     EYRIVTETQPSPQQMRDLIFAWRVARVVKSNAIVLARDGATVGVGAGQMSRVDSSGIA
                     VKKAGKRARGAAAASDAFFPFADGVEALAEAGIDTVIQPGGSVRDEEVVEEANRRGLA
                     MVFTGRRHFFH"
     gene            13165..13236
                     /locus_tag="VN178_11410"
     tRNA            13165..13236
                     /locus_tag="VN178_11410"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13197..13199,aa:Lys,seq:ttt)
     gene            13334..13406
                     /locus_tag="VN178_11415"
     tRNA            13334..13406
                     /locus_tag="VN178_11415"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13368..13370,aa:Glu,seq:ttc)
     gene            13457..13528
                     /locus_tag="VN178_11420"
     tRNA            13457..13528
                     /locus_tag="VN178_11420"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13489..13491,aa:Val,seq:tac)
     gene            complement(13629..>14612)
                     /locus_tag="VN178_11425"
     CDS             complement(13629..>14612)
                     /locus_tag="VN178_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWS81623.1"
                     /translation="TEAMANRDKGFIYDDDNLTVKEYLDRWLSDSVRGTVRESTYSRD
                     KYLVTNHIKPALGRLKLKNLNAPHLQGLYRDRMDSGLSGSTVQKIHHVLHKGLRQAVM
                     WNLIPRNPADSVKAPTPTPKEMHPLSAQEARRLLEAARGDRLEALYVLAVHTGMRRGE
                     LLGIKWGDVDLENATVRVRRTLTRNGTGYVLGEPKTKKSRRTVRITPQAVEALESHRV
                     KQSEEMLTSGSLYQEQDLVFAGTGGRLINPSNLRQRSFKPLVEHAGLPRITFNDLRHT
                     CASLLFQKNVHPKFVQELLGHASVSITLDTYSHMLPGMGNQAADAMVEALS"
BASE COUNT         2722 a         4389 c         4950 g         2551 t
ORIGIN      
        1 gaggtcctcg ccgaggaggt cggggcgctc gaggagcggg ggctcgaggt gcggggccgg
       61 ctcctggtgg acgggcgggc gcacctcata atgccctacc acatcgcgct cgactcgcac
      121 cgcgagatcg cgctgggcga cgccagaata gggacgacga accgcggcat aggcccggcc
      181 tacgaggaca aggtggcacg ctccggcatt cgcgtccagg acatcttcga cgagggcatc
      241 ttgaagacca agctcaaggc ggccctcagg gagaagaacg cgatcttcga gaacgtctac
      301 ggcgaggagc actacgaggt cgccgacgtc gcctcctggt tgctctcctt ttccgagttt
      361 atcgggccga tggtggctga tacgggaacg ttgttacggg aggcgctgga caacagggag
      421 cgggtcctgc tcgaaggggg tcaggcgacg ctcctggaca acgatcacgg gacctacccc
      481 ttcgttacct cgtcgaaccc gacggtcggg ggcgcggtcg ttggttccgg catccctgcg
      541 cgccacctgg agtacgtcgt cggcgtcacc aaggcttaca ccacgcgggt cggcgacggt
      601 ccgatgccaa cggagctctt cgacgagacg ggcgagacca taaggagcgt cgggcgcgag
      661 tacggtacta cgacgggccg cagcaggagg gtggggtggt tggatctccc ggccatcaag
      721 tgggccgctt cgctgaacgg gatcacccac gtcgccatca cgcttctgga cgtactctct
      781 gcggtagatg agatcaacat ctgcgtcggc tacgagatcg acggcgaacg cgtagaaggc
      841 tacccgatgc accagacgga tcttcatcat gcaaaacctg tctacaaggc gctacccggg
      901 tggggcgagg acatcacctg gtgcaggatg aggggggacc tacccgaggc ggcgcaggac
      961 ttcgtcggct ttgtggaggc cgaagtcggg gctcccgtgt gcatgatcag cgtcggaccg
     1021 gagcgggacc aggcgatcgt ggaaaggatc gggatctagg gtcgtgaggc catgaaggtg
     1081 ctggtagtgg ggagcggcgg gcgcgagcac gcgttggtcg aaaccctggc tgccagtccc
     1141 ctcgagcccg agatgtacgc ggcccccggc aacgtcggca tctcacgcat cgccgagacg
     1201 gtggacatcc ccgtagacga tctggtctcc ctcagggact actccgccga aaactccatc
     1261 gaccttaccg tcgtgggccc cgagactccc ctgatagggg gtatctccga ggcgttctgg
     1321 gggggcggct tgaagatctt cggtccttcg cgggcggctg cccgcataga gggctctaag
     1381 gtcttcgcca aagagctcat gcgccacgct ggcgtgccga cggcccgctt cgaggcgttc
     1441 gatcgcgaag aggcagcact ggcctacttg agtgcacatc acgactaccc catcgtcgtg
     1501 aaggccgacg gagccgcggc cgggaagggc gttaccgtgt cccgatcgca ggacgaggcc
     1561 gaggaggcgg tgagggcctc tttcgccggc aagttcgggg ctgcaggaga acgaatcgtt
     1621 atcgaggagt acctcgaagg gagggaggcc tcgatcttcg tcgtcacgga tgggcaggac
     1681 atactgccct tcctgcctgc ccaggactac aagcgtatct acgacggcga cgaagggccc
     1741 aacacggggg ggatggggtc atactcgccc atcatgtgga tggagccggc cacgtacgcg
     1801 gccatactcg aggacatcat acgccccacc atccaccagc tctccctgat cggggccccc
     1861 tacacgggcc tcctctacgc tggcgtgatg gtcacggaga cggggccgaa ggcgctggag
     1921 ttcaactgcc gctttgggga ccccgagacg caggtcatac tgccgaggct cggttccgac
     1981 ctcctggatc tgatgctggc ggcgggcaac gaggatcttg ccgggcgcga gatcgtctgg
     2041 tccgcggaca aggcggtctg tgtggtgctc gcctcggaag gctacccgga gacatcttcg
     2101 tcgggggatg agatctccgg cctggagaac atccccgccg gggtctacgt ctaccacgcg
     2161 gccaccgagg aacgcgacgg cagcttctac acggccgggg gcagggtcct gaacgtcgtt
     2221 gggacgggac cgaccatcat cgaagcgcgg gcccgcgcct acgccgcggt cgagcagatc
     2281 cacttcgaag gcatgcacta ccgtacggat atagccctgg aggcgataga gctggaggaa
     2341 ctttgagccc gagcgtagga atcctggttg gaagcgagtc ggatctcccc gtcctggaaa
     2401 agtgcacggc ccgcctggag ggttacggca tcgagtacga actcgaggtc cgctcggccc
     2461 accgtaaccc tgaaggcgtc tccgagtacg caagcacggc ccgcgagcgg ggcctgaagg
     2521 taataatctg cgccgccggc atggccgccc acctggccgg ggccgtcgca gcgcgcacga
     2581 acctgcccgt tatcgggata ccagttgcct cgggctcttt aggcggcttc gacgccctgc
     2641 tcgccaccgt ccagatgccc tcgggagtcc ctgttgccac ggtggccgtc aacggcgccg
     2701 ccaacgccgc cgtgctcgcg gcccagatcc tcgcgctctc ggatcccgag ctcgccgaga
     2761 ggttggacgg ttgatagaga aggctcgata ctcgccgcag gatagaggag agatccacgc
     2821 aacgctatca gagtggcata ctaacctcag aggtagggag tccgacccgt gatcgagcgc
     2881 tacacactcc ccgagatcgg ggccgtctgg accgaggagg cgaagtatcg tgcctggctc
     2941 gaggtggagc tcgcggtcag ccgggcgagg gcacggctcg gagagatccc ggctgaagag
     3001 gtggaggagc tggccgaaaa ggcggacttc accgtcgaac gtatccacga gatagaatgg
     3061 gagaccaatc acgacgtaat agcgttcgtg accaacgtcg ccgagtacgc cggggacgtc
     3121 gcccgtcact tccacttcgg cctgacgagt tccgacgtgc tggatacagc aggggcggtg
     3181 cagctaaagg aggccctcga tctcatcctc gacgaagcga gagccctgac gctgcttctg
     3241 gccgggatgt cgttggaaca tcgcgatacc gtgatggtcg ggcgtacgca cggggtccac
     3301 gcagaaccta cggtgctcgg gcacaagctc gcggtgtggg ctttcgagat ggagcggaac
     3361 ctggaccggc tcgctcacgc gcgtgaggtc gccgctgtgg gtaagatctc gggcgccgtc
     3421 gggacctacg cgaacgtcga cccgaaggtc gaggcgatca cgtgtcaaga tctcggactc
     3481 gcttcgtcac cggcctcgac ccaggtcgta cagcgcgaca ggcacgccga ggttctctcc
     3541 acgctcgcca tcctcggttc tacgatggag aagatagcgc tggagatcag ggggtcccag
     3601 cgtaccgagg tgcgcgagct ggcggagccg ttcgggcgcg ggcagaaggg ctcctcggcg
     3661 atgccccaca agagaaaccc gatcctggcc gagcgcctgt gcggtatggc ccgactgctc
     3721 cgcgccaacg ccgccgtcgg cttcgagaac aacgccctct ggcaggagcg ggatatctcc
     3781 cactcctcgg ccgagcgcgt cgtactcccc gactccacga tcctggcctt ctacatgctg
     3841 cgcaccacgt ctcgcatctt acggggtctc gaagtacacg aggagcgcat gctcgagaag
     3901 ctgaacagtg ggggaggcat cgtattctcc ggccgcgtcc tgctcgcgct cgtggagagc
     3961 ggcatgaacc gcgacgacgc ctaccgtgtg gtccaggatg ccgcgatgcg ggcctgggat
     4021 ggcgaggccg gcttcaggga gttgctggaa gcagatgacg acgttcgcga tcggttgggg
     4081 gatgatctgg atacgctctt cgaccccgca tacgcgctgc gcaacctcgg cgcggttttt
     4141 ggacgtgtag agaagctgaa aaggaggctg gaggatgact aaggaattac tctacgaggg
     4201 caaagccaag aaggtcttca ccaccgatga cgataacgtg cttttgcacc gctacaagga
     4261 cgaagcgacg gcgttcaacg cccaaaagcg cgggtcgtgg aaggacaagg gcaaaacaaa
     4321 cgcaaccatg agcgcggcga tcttcgccta cctggacacg cacggcgtcc ccacccactt
     4381 cgtcgagcag gtcgacgaca cgaccataaa gaccaggaag ctcgacatgt tgcccgtcga
     4441 gatagtggtc cgcaacgtcg cagccggctc cctatccagg ctgctcggct tcgaggaggg
     4501 aaggaagctc aaggccccca tagtagagct ctgctacaag gacgacgatc tcggagaccc
     4561 cctgctgaac tggtaccact tcagggaact cggcgtctcc gacgaggacc tggagttctg
     4621 cgaggagctt ggcctcaagg tcaacgaagt cctcgcgccg ttcttcgacg agcgcggcat
     4681 agtcctcgtc gacttcaaga tagaggcagg acgggatgcg gagggcaacc tcatgctggc
     4741 ggatgagatc tcccccgaca cctgccgttt ctgggacaaa gagaccggcg agaagttgga
     4801 caaagaccgt ttccggaacg acctgggagg cgtagaagac gcctacgccg agatgcttcg
     4861 aagagtcgcc gacatcacac agagaggcgc ggtagattga gcagcttttc agctatcagc
     4921 tttcagccgt cagctatcag cagggaggtt ctattcgttg ctcccctgcg catcgcggac
     4981 cgttggggca gagaacatcc caccagaggc cgactcgcac aacgagagca acttgcgagt
     5041 cgaagggaag aaagctgaag gctgatagct aatggctgag agcttaaaga tccgggtcct
     5101 cgtccgtccc aaggccggta tcctcgaccc gcagggcgag gcggtgaaga acgcgcttcc
     5161 tgccctcggt ttcgagggag tcaggaccgt ccacgtcggc aggctcgtcg agatggaagt
     5221 cgagagcacc gaggaggtcg acgccatgtg ccgcaggctc ctctccaacc cgacgataga
     5281 agagtacgag tgggaggtcg tgagatgagg gagtgggggg agcgaagcgg agcgacatga
     5341 ggattggagt cactgttttt cccgggtcca attgcgatag ggacgccctc tacggtgtgg
     5401 agcggatggg cgctgaggcg gtcgagctgt ggcacgccga cgccgacctc aagggcgtcg
     5461 acgcggtgat cgttcccggc ggcttctcct acggcgatta tctgagaccg ggcgccatcg
     5521 cccgttttgc gaacgtcatg ggcccgctgg aggagttcgc gaagagtggg ggaccggttc
     5581 tcggcgtctg taacggcttc caggtcctga ccgaggcgca ccttttgccg ggcgctttgc
     5641 ttcgcaacac gcacatgcgc tttgtctgtg accgcgtcag ggtacgggtc gaatccgtag
     5701 atacgccctg gaccgcgggc tgcgagcccg gtgaggagct cactctaccc gtcgcccaca
     5761 acgaggggaa ctacttcgcc gatcccagga cgatcgtgga gttggagaac gaagggcgtg
     5821 tcgtactgcg ctacctggag aacccgaacg ggtcggcgaa cgatatcgcg ggcgtctgca
     5881 acgaggaccg caacgtcgtc ggcatcatgc cgcatcccga gagggtctgt gactccgccc
     5941 tcggctccga tgagggcctg aggatactgc ggtccgtgct ggtgggggcg ggcgtctgaa
     6001 tatccaggag acatacagtt ctctggggct ctcggacttc gagtacgacc gcatcctgga
     6061 gctgatggga agggcgccga atttcctgga gctctcgctg ttctccgtga tgtggagcga
     6121 acactgcggc tacaagaact cgcgcccgct cctcggccgc ttcccgaacg aggggaagcg
     6181 ggtgttgcag ggaccggggg agaacgccgg ggtggtcgag gtgggggacg ggtgggcgct
     6241 cgcgttcaag atggagagcc acaaccatcc ttctgcggtg gagccttacg agggggcggc
     6301 gacgggggtt ggtgggatca tccgcgacat cctggccatg ggcgcgcggc ccgtggcgct
     6361 gctcgactcg ctgcggttcg ggccgctgga tgaggagcgc aaccgctacc tctttcggga
     6421 ggtagtgcgt ggcatcgggg ggtacgggaa cgccgtcggg gtaccgacga tcggggggga
     6481 ggtcgagttc gggcgggcct actccggtaa cccgctggtc aacgctatgt gcataggctt
     6541 gatccgggta gaggatctcg tccgttcgag cgccactggt acaggtaacc tcgtcgtcct
     6601 gctaggttcg aagaccgggc gcgacggtat acacggggcc accttcgcct cggacgagct
     6661 caccgacgaa tccgagtcga aaaggtccaa cgtccaggtc ggcgacccgt tctgcgagaa
     6721 gatgctgatc gagtgttgcc tgaagctgct tgatgaggac ctgctagtct ctttgcagga
     6781 cctcggcgcc gccggcatca cctcctcggc ctcggagatg gctgcgaggg gcggggtcgg
     6841 tatcgagatc gagaccgaca tggtgcccct gcgcgagagc gggatggagc ccacggagat
     6901 catgatctcc gagtcccagg agcggatgct cgccgtcgtc gagcccgaaa agatcgacga
     6961 ggttctgtgg atcgcggaac gttacgaact cgtcggggcg gtcgtcggtc gcgtcgccga
     7021 gcacggcgac ctgcgcgtga tccacgacgg ggaggtcgcc ggcaccgtcc cggcagaaca
     7081 cctcgccgac gctcccgtct acgagcgcga cgtggtgcga ccgtggtacc tggacgacgt
     7141 acccgaactc gacctggacg agattcccga gcccgaagat tacaaggccg tgctcttgca
     7201 gatgctcgga caccctaacc tctgttcgcg gcgtcccatc ttcacccagt acgaccacca
     7261 ggtaggcacg gacagcgtgg tcttgcccgg cgctgacgcc gcggtgatgc ggatcaaggg
     7321 cacggatcgc ggcttcgccg ccaccactga cggcaggggc cgccactgct acctcgaccc
     7381 gaggggcggc ggggccgcaa cggtcgccga agcctaccgg aacctctctt gtgtcggggc
     7441 agagcccgtc gccgtcaccg actgcctcaa cttcggcagc ccgaagaaac ccgacggcta
     7501 ctaccagctc gccgagtgca tcgaggggat gtccgaggcg tgcgaggcct tcgggacgcc
     7561 ggtcgtgagc ggcaacgtca gcctctacaa cgagaccgac aaaggcgcca tctacccgac
     7621 ccccaccgtc gggatggtcg gtatcctgga ggacgtagag cggcacgctt cgcctggctt
     7681 caagcgcgag ggggatctgg tggtggtgat cgggggagga tctcccgcct ccctcgacgg
     7741 ctctgaatac ctggagataa tccacggtcg tgtcgcaggg agacctccgc ggcccgacct
     7801 cggtcccggg aaggagacgg cggacctggt gcgcgagatg gtccgttccg ggctcgtgga
     7861 tacggcacac gacatatccg gcggcggaga gatctttgct gtcgcagaga tggcgctggc
     7921 cggcggactc ggaatcgagt acgaggagga cgggctcgaa cgcataaggg ctggccgagg
     7981 aagaaccgat gttgctttgt tcggagaggg gagcggtgcg ttcgtcgtcg cggttccgga
     8041 agaacgctgg ggtgaagtgc aagatgcact ggtagggttc gcttacaacg tcgtcggtac
     8101 taccggcgga gagaggttcg agataggcga cctgatagac atagagctgt cggacttgag
     8161 ggaagcatac gagcgtgacc tcttcgaaag ctatgcgccg gagggcggac acattgggta
     8221 gcgtagagaa gggagacgca aacggtgggt gcgcttgacg tagactacag ggattcggaa
     8281 ggcaagccga aggaggcgtg cggcgtcttc ggcctctact cccgagagct ctcgggggag
     8341 ctggcccagc gtacgtactt cgggctctac gccctccagc acaggggcca ggaatcagcg
     8401 ggcatcgccg tctccgacgg ggagcgcacg accgcgatgc gcgacatggg gctcgtctcg
     8461 caggtcttcg acggaccgcg cctcgcggcc ctcgaggccg ggcacctggc cctcggccac
     8521 gtccgctact cgacgaccgg ctcggcttcc tgggagaacg cccagcccga gttcggcggt
     8581 cgcggcgacg taaacgtggc cgtagcccac aacggcaacc tcgtgaacgc cgagaggttg
     8641 cgggatggcc tggcagaaga cggcttcagg ttctactcga cctcagacac gacctacatc
     8701 gcggcggcgg ccgtgagaga gctggagcgt ggactctcgg tcggcgaggc ggtcaggggt
     8761 gcgatgcgcc acttcgaagg cgcttactct gtggccatga tcttccgcga caaactcgtc
     8821 gcgttccgcg atcctcatgg ctttcgtccc ctctgcatcg gtgaggtcga gggcggttac
     8881 gccgtctcca gcgagacgtg tggcctggac attatcggtg ccaccttcct gcgcgacgtg
     8941 gagcccggag aggtcatcgt tatcgacgac gatggcctga cgagctacac ggccgagagc
     9001 ccgcgccgcg ctctgtgcgt cttcgagtac gtgtacttcg cccgacccga ctcgcgcttc
     9061 gacggacgcg aggtcgcggg ctccaggctc aggatgggcg agctgctggc cgatgaggct
     9121 ccggccgagg ccgatgtggt gatccccgtc cccgattcgg ggatgatggc ggcgatcggc
     9181 tactcgcagc ggagtggcat cccttacgcg gaaggcctca taaagaaccg ctacgtgggg
     9241 aggaccttta tccagccgac cgacggtatg cgccagctcg gcatcaggct caagctcaac
     9301 ccgctgccga gtgtaataga ggacaacagg gtggtcgtcg tggacgactc catcgtacgt
     9361 gggaacacca gccgcaagct ggtcgagctg ctcttcgagg ccggcgctgc cgaggtccat
     9421 ttcagggtct cttcgccccc gatcaaaggc ccctgctact acggcatcga catggatagt
     9481 cctgaccaac tcgtcggcgc gaacctgacg gtggaagaga tccgggccca gatcggggct
     9541 acctccctcg cctacctctc tgtcgatgcg atgatagccg ccacacaaca gagaaaggac
     9601 cgcctgtgcc gcgcctgctt cgacggcgac tacccgacga cgggcacatc gagcaagttc
     9661 gccctggagg acagaaccca atacgccgct accaggggcg gatgaattgc acctccgtcc
     9721 ctacgagcct tttggcacac gtgccttaga ggggtgcacc gttgcctgaa aacagctacg
     9781 agggcgctgg cgtctcgatc gaacgcggcg agcagtccgt cgccctcttg cgccccgccg
     9841 tcgagaagac ccacggtccc gaagtcctcg gcgcaaccgg cggcttcgcc ggcctttacg
     9901 ccctcgccgg ctacagggac ccggtcctcg cctcgaccat cgacggcgtc ggcacgaagg
     9961 tcctcgtcgc cagagaggtg ggacgctact actctttagg cgctgacatc gtcaaccact
    10021 gcgccaacga cgtgctcgcc acgggcgcgc gtccgctctt cttcctcgac tacgtggcca
    10081 gtggccgatt agatcccgag gccgtagccg gcatagtcag cggcgccgca gaatcctgcg
    10141 ggagactcgg tgtcgcgctc atcggcggcg agaccgccga gatgcctgac gtttacggcc
    10201 ggggagactt cgagatagta ggcgtgtgcg taggggcgtg cgagcggggc gaggtagtca
    10261 gcggtgagcg ggtacggccg ggcgacacga tactcggtct cgcgtccagc ggtctgcata
    10321 ccaacggcta cacgctggcc cgcaaggtga tcgaggatgc aggggtcacc tacgacgatg
    10381 ttcccgaggg cctggaccgg tccgttggag aggtctatct ggagccacac cgggcctatg
    10441 tacgggaggt agatgccctg cgcagtgccg tcgagattcg ggggatggcg cacgtgacgg
    10501 gcggtgggct cgtggggaac ctcccgcggg cactcggggg attaggggca cggttcgatg
    10561 tgggatcatg gtcagaaccg gcggtattcg ggctcatacg tactctcggc ggcatacctg
    10621 agggcgagat gcgcagggtg tacaacctcg gcgttggttt ctgtgcggtc gtgtcgcgcg
    10681 aagaggcaga ggcgggactc caggcgctgc tcgggacagg ttgcgatgcc tggcggatcg
    10741 gggaggtcac agaagccggg ggcgtagagt ttgcctgagc ggctacccga agtctcgcgc
    10801 ttcgccgtgt tggcctcggg ttcgggaacg aacctgcagg ctttgctgga tgcatatccc
    10861 gagaacctcg tcgtcgtcgc cggggacaat aaggaggcgt tcgctttcca gcgtgcccgg
    10921 cgggcaggcg ttcccgtcga gcacgtcgac cccgaggggt tcgtcggcaa ggaagactac
    10981 gacagggagc tcgcagacag gatcgaagct cacgacgtcg ggctggtggt gggggcaggg
    11041 tacatgcgga tattgtcgcc gctctttctc gagcgcttcc ccgccgtttt gaacgtccat
    11101 ccttcgctct tgcccgagtt tcgcggactt cgcgccgtgg agcgggcgtt ggaggctggg
    11161 gtcgaagaga ccggggtgac ggtgcatttc atggtcgagg aagtggatgc cgggcccgtc
    11221 gtcgcccagc agcgggtgcc cgttctgccc ggggacaaca aggagacgct actggaacgg
    11281 ctgcaccccg tcgagcacgg gctgctcgtc cgggccgtcg cgaactattt tctggggagg
    11341 gtggaggtat gaagcggcgg gcgctgatct cggtgtcgga caagaggggg atcgaggact
    11401 tcgcgcggcg gctctccggg atggggttcg agatcatctc gaccggaggt accgcgaagg
    11461 tgctctctga agccgaagtc atggtcatcc ccgtctccga ggtgacgggc acgccggaga
    11521 tactcggcgg gcgggtcaag acgctgcacc ccaagatcca cggcggcatc ctcgccgacc
    11581 tcacagaccc cgaccacgtc aagcagctcg tcgagcagga tatcgggccg atagacctgg
    11641 tctgcatcaa cctctatccc ttcgaggaga ccgtcgccgg cggcgccccc gagaaggagg
    11701 ctatagaaca gatagatatc ggaggacccg cgatgctgcg ggccgccgcg aagaacttcg
    11761 ggtccgtgac cgtcgttccc tcgcctgaat actacgaaga ggtactcgcg gccctccaaa
    11821 gcgagggaca ggtccccgag gagacccgcc gtcgactggc gctcgcggcg ttccggcgga
    11881 ccgccgagta cgacgggaag atctctggct ggctcggtag gcgtgtcgaa ggggagtccg
    11941 agtcttccga cttcgaggcg gaaggcttcc cggtgcgtcg aacggtagat tacgagcggg
    12001 tctctgcgtt gcgttacggg gagaacccgc atcaggctgc ggcctattac gccgaggcgg
    12061 gtgcagacca tttgttgagc ggtgtggaga agttgcaggg acgcgagatc tccttcaaca
    12121 acctctacga cctcgacgcc gcacgcaccc ttctcgccga ccttaccgac ctgggagccc
    12181 aggccgccgt catcgtcaag cacgccaacc cctgcggcgc cgccgtcgca gactccttgg
    12241 aggatgcata ccgcaaggcg ctggcctctg atccacaatc tgccttcgga ggcatcgtgg
    12301 ccctgaacgg cgaggtagat gggaatcttg catccgagat ctccagcata ttcacggaga
    12361 ttctcgtcgc cccagggttc acccaggagg ccagggagat cttctccgca aagcccaacg
    12421 tgagggtgct cgtcgcaggc ccgctcgttc gtcccgcgct ctcgtccagg cacgtaaccg
    12481 gcggagtcct cctccagaac tcagacgcca tcgaggacga ctcggagtac aggatcgtca
    12541 ccgagacgca accttcgccg cagcagatga gagacctcat cttcgcctgg cgcgtagccc
    12601 gcgtagtcaa gagcaacgcc atagtcctcg ccagggacgg ggccacagtc ggcgtcggag
    12661 cgggccagat gagccgggtc gactcgtcag gaatagcagt aaagaaggcc ggcaagagag
    12721 cacggggagc cgccgccgcg agcgacgcct tcttcccctt cgccgacggc gtggaggccc
    12781 tggccgaagc cggcatagac accgtcatac agcccggcgg ctcagtacgc gacgaggagg
    12841 tcgtcgaaga ggccaaccgc cgagggcttg cgatggtctt taccgggcga cggcatttct
    12901 ttcactagaa cggcttgtgg aaagcatcag gctacaggta ttgcagcgca cagtcacgtc
    12961 aaatgggata cgtgctctcc tgtgtcacga ctcagccacc tatcatgtca ttcctgcgga
    13021 agcggaaatc cactgcagga gaagtttcga gcctccaacc agatgcgact tgccagagtg
    13081 agtatcagct ctagtgagtg gtgcggcagt ggggactctg ggtcgaagac agagtggttt
    13141 tcgagtaaac tctggataga gtctgggcca gtagctcagt ggtagagcag gggactttta
    13201 atcctcgtgt cgtaggttcg atccctacct ggctcacgaa ttctctggcg aggaccctgc
    13261 ctcaccccag ggacgatctg ttaaactctc gactgcgaaa ccggcagtgg ctccggccct
    13321 gcccgtctcg tgagccccca tcgtctagag gcccaggaca ccgccctttc aaggcggcgg
    13381 cacgggttcg aatcccgttg ggggcacctg ttagccggtc ggcaacgccc aagctgacag
    13441 gctgaaaacc tactttgggc gattagctca ggggtagagc gcctccctta caaggaggag
    13501 gtcgctggtt cgaacccagc atcgcccact tctaaaagtt ctatatttgc aggtaaaatg
    13561 cgtgagcggg gagaaggccc cgatgtcaca tcggggcctg ggtgcagcaa cggtgcagca
    13621 acgcagagct agctgagtgc ttctaccatc gcgtctgcgg cctggttgcc catgcccggg
    13681 agcatgtggg agtaggtgtc gagggtgatg gagacggagg cgtgtcctaa tagctcctgc
    13741 acgaacttcg ggtgtacatt cttctgaaat agcagtgagg cgcaagtgtg acgcaggtca
    13801 ttgaaggtga ttcgaggcag acccgcgtgc tctacgagcg gcttgaagga tcgctgtcgg
    13861 aggttggaag ggttgattag acggccgcct gttccggcga atacgaggtc ctgctcctgg
    13921 tagagagatc cagatgtaag catctcctcc gactgcttta cccggtggct ttcgagagcc
    13981 tctactgcct gtggagtgat gcgtacggtg cggcggctct tcttggtctt cggctctccg
    14041 aggacgtagc cggtcccgtt gcgcgtgagc gtccggcgca ctctgacggt ggcattttcc
    14101 agatcaacgt ccccccactt aataccgagt agctcaccgc gcctcatccc ggtgtggacg
    14161 gcgagcacat agagggcttc gagccggtca cctcgtgccg cttcgagcaa acgtcgggct
    14221 tcttgcgccg atagcgggtg catctctttc ggcgtaggcg taggtgcctt cactgaatca
    14281 gcggggttgc gggggatgag gttccacata acggcttgcc tgagcccctt gtgcaggacg
    14341 tggtggattt tctgaaccgt cgagccagag agtcccgaat ccatgcggtc acggtagagg
    14401 ccctgaaggt gaggtgcgtt caggttcttg agcttgagcc gtccgagagc gggcttaatg
    14461 tggttggtca caaggtactt gtcgcgagaa taggtggact ccctgaccgt gccgcgcacg
    14521 gagtccgaga gccaacggtc caggtactct ttcacggtga ggttatcgtc atcgtagatg
    14581 aagcccttgt cccggttcgc catcgcctca gt
//
LOCUS       DATMVF010000221        21763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_71680, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000221 DATMVF010000000
VERSION     DATMVF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 21763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21763
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71680"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1068)
                     /locus_tag="VN178_11430"
     CDS             complement(268..1068)
                     /locus_tag="VN178_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81624.1"
                     /translation="MPDRELERELKELGGFIDYPPTPDAARAARRVLDEENSRPRRFL
                     TAFPAMRWVAVAAAFVLVVAVPTLSPGLRATIGDWFVAEGVHNAGEPAVDAGSPERQT
                     EAAAPSAGVSKSGESLAARFSGERITLQEARARMEGALLLPRIPNLGKPDEVYAAGTS
                     EKNGVVLVYRDGLPPLGGAGISLVLTETPGDVESAYLGGKSTVGSELERVSVDGDPGY
                     WGSAGRIPSAMDPRLPGGVLLWEKGGYALRLEADLHKEQAIRIAESVR"
     gene            complement(1058..1549)
                     /locus_tag="VN178_11435"
     CDS             complement(1058..1549)
                     /locus_tag="VN178_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS81625.1"
                     /translation="MVRGYQEVAFRTAYLITGDASEAEEATQEAFVKAYRALGRFRSG
                     ARFRPWLLAIVANEARNRRRAAARRAGLFLRAAREATPENNPASPEAAVLAAERREEL
                     LEALGGLREEARWAIGCRYFLGLSEEETAAILGCARGTVKSRLSRALKSLRTEIAKEG
                     DAR"
     gene            1770..3344
                     /locus_tag="VN178_11440"
     CDS             1770..3344
                     /locus_tag="VN178_11440"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWS81626.1"
                     /translation="MQDERLGENTTAGKIEGLGHLREAAAHPAPEQAVERQRERGKLT
                     ARERVELLLDPGTFVELDRYRVHRSYNFGLEENRPLGDGVITGYGEVSGRKVCVFSQD
                     FTVFGGSLGEVCAQKICKVMDLAISTGCPIIGINDSGGARIQEGVVSLAGYADIFHRN
                     VLASGVVPQISVIVGPCAGGAVYSPAITDFVFMVEETSHMFITGPDVIKSVTGEDVSF
                     EDLGGAATHNTRSGVAHFSAPDEETCFDDVRYLLSFLPENNLESAPYFRPTDDPERMD
                     EDLARLIPDSPQRPYDIRGAIEGVIDDGEFFEVQEGWAQNLVVGFARLDGRSIGVVGN
                     QPSVLAGTLDINASVKGARFVRFCDAFNIPLLTFVDVPGFLPGTDQEWGGIIRHGAKL
                     LYAFSEATVPKMTVITRKAYGGAYDVMCSKHIGADVNVSWPTGEIAVMGASAAVGVIF
                     RRQISEAEDPDARREELIEDYEEQFNNPMVAAEMGFIDDVIDPRETRPYLIQALQMIR
                     TKRPDRPPRKHGNIPL"
     gene            3408..3650
                     /locus_tag="VN178_11445"
     CDS             3408..3650
                     /locus_tag="VN178_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81627.1"
                     /translation="MPDDDRKTLGSPFPLRIQPAPNLEEVAAIVAALETLSGGGQRDS
                     ASLKRSRWRLAGLLGHALEPGIEPDGSLWAYSRWEG"
     gene            3656..5395
                     /locus_tag="VN178_11450"
     CDS             3656..5395
                     /locus_tag="VN178_11450"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWS81628.1"
                     /translation="MFGKVLIANRGEISVRIIRALREMGVRSVAVYSDPDREALHASL
                     ADEAYHVGPAPAAVSYLNKERLIEVARKSGAEAIHPGYGFLAESAPFARAVEGAGLVW
                     IGPHPGAIEAMGSKVESRRIMAEAGVPMVPGTRDSITFAKQISLFGEEHGFPVAVKAS
                     AGGGGKGFAVARDASESASAFERASREGEAYFGDGSVYIEKYLSAPRHVEIQVVRDRH
                     GNAVHLGERDCSIQRRHQKLVEECPSPAIDPMMRDAMGKAALAAAEAVDYDNVGTVEF
                     LLEEGEATGAFYFLEMNTRVQVEHPVTEEVTGIDIVKTGIRIAAGEELPFSQRDISWR
                     GHSIEVRLNAEDASNDFAPSPGVVTAYVEPSGPGVRVDSSLRGPGVVAESYDPLFAKL
                     IVRGSDREEALARLARALAEFRVEGISTTLPFYRAILDDEVFVSGDYTTGFIAERMHR
                     LEIQPAVTGETGTSPEKHAREVEVEVNGKLFRVRVFGDEGTTVGPPRRRRGETRRAST
                     NEGAIRAPMQGTIVKVLVEEGQEVAADEAVCVLEAMKMESEVRSQKAGTVSEVRVEAG
                     QTVRSGDILISVE"
     gene            5404..6483
                     /locus_tag="VN178_11455"
     CDS             5404..6483
                     /locus_tag="VN178_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81629.1"
                     /translation="MTEHTAEYEWHGDGEEGEVVLYAPDDSAFERALPAARLPGVESP
                     VYAAASQGDFGWAAASATHAAPDLLSGPLRGLLLVAGTRSENLGVPPQELRDRIRREL
                     PEAGARLPSLNESGAGRFCESGARAAAEDDLIEEDDLPLLDVCPGDADALGRRALAAA
                     PRAWAGRVEPDLRLVTEVLDTEGAESLGLEEGALAIAVKVDAGDLGRLALAAHKERIL
                     SRTQSGDFGSTPELPAAPASSGEVEDLVAALYAATNFADARAARTVWMLRRVLGDAAG
                     GLEILAAWRTGGIEEQEGRLVHRRGFAWAENGHVLVCGSYAAAGTGNMWASAPPFGVP
                     EEEGRWPWEEAGLLERLVTLDPPEG"
     gene            6486..6785
                     /locus_tag="VN178_11460"
     CDS             6486..6785
                     /locus_tag="VN178_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81630.1"
                     /translation="MDLLMVRDRSTGRFVYTERLERRSGETSWEYVRRSVRREARIRT
                     RFDGDTMEVIVGWDVDSVEAFLRAYPEYRTDGDSDAASGRSREHEERTEGDAVDR"
     gene            6775..7089
                     /locus_tag="VN178_11465"
     CDS             6775..7089
                     /locus_tag="VN178_11465"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HWS81631.1"
                     /translation="MTGEVLDRFSELVTTALGLVAALAWNTAIQNLFDTVFGGSGSKL
                     AGQFLYATLITLVVIFAAIAVSRAQKERNRPRRNEEGRGRFVDRDRDRQAAGAGSKPA
                     LT"
     gene            complement(7082..7582)
                     /locus_tag="VN178_11470"
     CDS             complement(7082..7582)
                     /locus_tag="VN178_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWS81632.1"
                     /translation="MSANGFTKMSIYGMNMDLFSSSPIVILKVEDENRYLPIWIGQAE
                     ARSILFKLQNQEFVRPLTHDLALSLVNELGGSMERITVTELRDSTFFATIRLEIEGKT
                     VEVDSRPSDAIALAIRSGAEIFASDEVIEEAGVVFEEAIEDTPEDEVVDKFKDWMNQV
                     SPEDFK"
     gene            7736..7809
                     /locus_tag="VN178_11475"
     tRNA            7736..7809
                     /locus_tag="VN178_11475"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7770..7772,aa:Ile,seq:gat)
     gene            complement(8985..10316)
                     /locus_tag="VN178_11480"
     CDS             complement(8985..10316)
                     /locus_tag="VN178_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWS81633.1"
                     /translation="MESTRSMQADTQVDSRELWDAVDAHLVRYGGDFVPELVSSASGS
                     RLYTVDGRSILDFTSGQMCATLGHNHPAIVAAMEKACREAIHLFSGMLAPPVFLLARE
                     LAGMLPPALQKVLLLSTGGESNEAALRLAKLKTGGFEVLGFTGSWHGMTAGASSSTYS
                     AGHKGYGPAMPGSMVLPAPNCYRCPIRHCQDRCDMTCLEVGVGLADSQSVGAYAAVIA
                     EPVLSVGGIVPLPKGYLARLKRACEERGMLLILDEAQTALGRVGADFAFEAEDVVPDV
                     LTLSKTLGGGLPLSATVTSVAIEEECYEKGFLHFTSHVSDPLPAEVGLAVLRVLAEER
                     LAERARLMGDRLRSALLELQARHEPIGDVRGRGLMLGVELVKDREGRAPDEKLGARVT
                     RRCLELGLNMNIVKFKGLGNVFRLAPPLTVTTDEIDEGVSIIDQALTECGK"
     gene            complement(10680..12089)
                     /locus_tag="VN178_11485"
     CDS             complement(10680..12089)
                     /locus_tag="VN178_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWS81634.1"
                     /translation="MQTEAKKTHQTLLFRSATDAAQVVRRKEISSRELTATLIKRIEA
                     VNPALNAVVELRREAALREAVAADEATDSGDATGPLHGVPMTIKDGFNVAGLHTTWGN
                     PAFEGFVAGSDATVARRLKEAGAIIVGKTNVAFMLGDFGQTANELYGVTNNPWDTTRT
                     PGGSSGGAVAALSAGMTFLEYGSDLVGSIRIPASFCGVYGLKPSAGIVPLGGFHPPGP
                     PPPPSEMTYMSAVGPLARSPRDLRTALSVTAGPEKQAARAYSWTLSPPRHARLEDFRV
                     GVVLDHERAHVSGEVATPLSNAVDALARAGASVVEGWPDGVDPVQHYESFGFHVQAFF
                     AFQQPGEDFATFPEFVDHENRRMASRAAWNRYFDDMDVFLCPVSFTPAFPHDERPFEE
                     RTIATPEGERSYSDQSFWVSHASLPGLPAVAAPIGRTPGGLPVAAQILGPLYEDDTAI
                     TFAELLGDVIVGYEPPPLS"
     gene            complement(12102..12923)
                     /locus_tag="VN178_11490"
     CDS             complement(12102..12923)
                     /locus_tag="VN178_11490"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWS81635.1"
                     /translation="MLSIGDFARHGRVSVRMLRHYDAIGLLKPAFVDRVTGYRSYEAR
                     QLSRLNRIVALKGLGFTLQQVRSVLDDEVSVEELRGMLRLRHAELQSQIETDTARLAR
                     VEARLRIIEREGAMPADEVQIKRIPGLRVAELTGTAAGFEPESIAPVIGPLYDELRGR
                     LERAGLTPAGPDLAYYEDSPDGDGVLIHATIPVNVGPGEDHDFQVVNLPEIEQAATIL
                     HRGSMDNVMPTIQALARWIDANGYRPVGYNRELYIELGENRDAWVTELQEPIVAR"
     gene            complement(13029..13199)
                     /locus_tag="VN178_11495"
     CDS             complement(13029..13199)
                     /locus_tag="VN178_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81636.1"
                     /translation="MAISDQFRRYPAGRRSKRFRATAPSLRPEYHTGYYGAYAFDTDG
                     NNVEAVHHTVPT"
     gene            13274..14056
                     /locus_tag="VN178_11500"
     CDS             13274..14056
                     /locus_tag="VN178_11500"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81637.1"
                     /translation="MMSDRERSLTVFLELPERVAIVTGGARGIGFAIAERLSRAGARV
                     VIADIDEESAVAAAERLRDGGAEAVEAVADITNPGEASAMVDRAIHAFGKLDVLVNNA
                     GITGRDASLSEVTDEDWERVLKLNLTAAFYCCRAAIPRMREQRSGAVVNVASISGKEG
                     NPNMIPYSVSKAGLICLTKALAKEVIHDGVRVNCVAPAVIETPLLGQLRPEAIEYMTS
                     KIPMGRMGRAEEVAAVVHFLASDDASFVTGQCYDVSGGRATY"
     gene            14232..14849
                     /locus_tag="VN178_11505"
     CDS             14232..14849
                     /locus_tag="VN178_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS81638.1"
                     /translation="MGKVCTGHSTSLDGFIAGPDDGPEAPMGLGGERLLGWYAAGDTE
                     YRLPGTEMVFKVSAQSAELLRETRRTTGALVTGRRTFDLTRGWGGRHPLDVPVFVVSH
                     TVPQEEWVHEGSPFTFVTDGLESAIERAKAIAGDKDVGVIGASLVQQCIRAGLLDEIH
                     LDLVPVLLGDGVRLLDYPGTEPIELESTRVIEGAGVTHLTFRVVK"
     gene            15222..15755
                     /locus_tag="VN178_11510"
     CDS             15222..15755
                     /locus_tag="VN178_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81639.1"
                     /translation="MVLSTRLRVLLVVLVMALGAVGVLGAVGSASPNNSEDAHHRDDK
                     GRTTLTVVGKNPENELLDLGAQGPSQGDLRVANAPLYDESGKERIGRFDLFCAITDPA
                     DEPSEKAHMAQCTKTFTLPAGQISVQGVEAYPDLSGLAPGVNAISGGTGEYAGVEGEQ
                     RFEVRGKKVIDTFHFID"
     gene            complement(15752..16126)
                     /locus_tag="VN178_11515"
     CDS             complement(15752..16126)
                     /locus_tag="VN178_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81640.1"
                     /translation="MEPPPIALPLGIVGLYLRTRLEDTPGFRALENAGDVAEAGKGAE
                     MTEETAGAPLKQLSKTRPGPPKLPLLSDMFAVVGGGLPIETGRLRKGAAIRNRHRGAI
                     NPSNSREEGPAFPFSSLRLVQH"
     gene            complement(16311..17663)
                     /locus_tag="VN178_11520"
     CDS             complement(16311..17663)
                     /locus_tag="VN178_11520"
                     /inference="COORDINATES: protein motif:HMM:NF005812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWS81641.1"
                     /translation="MEAKDTRVSKLAEMDRRYVWHPMARLSQPGAPMMVAEGDGAWIT
                     DTEGNRYLDGMAGLWYANVGYGREELARAAYEQLQKLPNYPLTLSHEPAVRLGEKLNE
                     WLDDEYVFFFSNSGSEANEVAFKMTRQYHLQRGEGSRHKFIARYRAYHGTGGGALAAT
                     GMGQRKYRYEPLSPGFLHVQPPMRYRCGYCADRPACTMECAREIERTINWEIAESVAG
                     VIMEPIITGGGIILPPEGYVENVAEICRESGALLIMDEVICGFGRTGKKFGHQHYGVK
                     PDIVTMAKAITSGYLPLSATAIKREYLEAFKGTEEYDHFRHVNTFGGNTTSCALALRN
                     LEIMEDEGLPERSAQMGEKLGEKLSDLEDHPNVGEIRHKGLLLGIELVEDKETRKPAE
                     GLTTEVFSRCKERGLIVGKTGDTTAGFNNDITLSPPLIVTEDDLEFMSGAIREVVNTL
                     "
     gene            complement(17940..18755)
                     /locus_tag="VN178_11525"
     CDS             complement(17940..18755)
                     /locus_tag="VN178_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWS81642.1"
                     /translation="MENGSYRFAIGEASRAVGVAPGTLRVWEREGLVSPSRSKGGTRY
                     YSKEDVERLKRIKQLKTIHRLNSAAIRRELESTGSSTLPQEGREDDQPAAAGVGLRLR
                     LLRTRHRMTIRELARTTGLSPSFLSALERGETGASIASLSSIVKVYGMNMRDVFDADL
                     KKSSLLVRPEDRPTMQWSNGVRYEELAASGSLMNPSFVYVPPHSGSGGFYSHDGEEFV
                     YVLSGSFYVELEERETFRLEARDVLYFPSTSPHRWWTEDEEAEVIYVNSPPTF"
     gene            complement(19012..19200)
                     /locus_tag="VN178_11530"
     CDS             complement(19012..19200)
                     /locus_tag="VN178_11530"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWS81643.1"
                     /translation="MSASTQRSKAQRRGELYREGDTLVLPNAWDGMSARVFEDCGFPA
                     IATTNAGIAYTFGYPDGE"
     gene            complement(19209..19538)
                     /locus_tag="VN178_11535"
     CDS             complement(19209..19538)
                     /locus_tag="VN178_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81644.1"
                     /translation="MGSELGISDEKIVALEGYAESPLYGEMERAALEYADAITLSDRE
                     VGEELFERVRAFFDDDALVELTAVIAWENSSSKFNRALRVPSQDLWRGSRDYRASGAD
                     RPGEDDI"
     gene            complement(19557..19859)
                     /locus_tag="VN178_11540"
     CDS             complement(19557..19859)
                     /locus_tag="VN178_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81645.1"
                     /translation="MYREYGRRLVENRDERRLEYVARIEGVDPERAEKRIRAVLEAQA
                     EKWGGPLSNHLLYARRPTIFRGVRGMWGGLEQSGLLDPRLVALVNRRVAALNGCEF"
     gene            19953..20684
                     /locus_tag="VN178_11545"
     CDS             19953..20684
                     /locus_tag="VN178_11545"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWS81646.1"
                     /translation="MKDSANVARIAWALSDPVRLSVMQVLMGGPATVSELVSATGASQ
                     PNASNHLKVLREQGLVRSERRGRQVIYEIPDTSVAQLVESLTAVAGGPEAPVFGTDPM
                     AEARTCYDHLAGRLGVGIYGALVDSDALVPTAEVRGDLRLGPEATRVFGAIGVDPAKT
                     AQEAGRRRFAFACPDWTERMPHVGGALGAAICERFFEEGWVRRGERTRAIALTGGGRS
                     AVRERFGLEIGETTGFDRVGEGGDT"
     gene            complement(20778..21173)
                     /locus_tag="VN178_11550"
     CDS             complement(20778..21173)
                     /locus_tag="VN178_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWS81647.1"
                     /translation="MDFETNAAGKQEVLASGTHGRILVARTWFGRTRKEFADEYLRYN
                     YENGVLEVEKKPGCLGMQQFRKIVGDVAEFTTISYWESTEAMEAAHGDDLRPTHLEKD
                     EEYLLELPESVEVSELYVNDWLVSPNERP"
     gene            21374..>21763
                     /locus_tag="VN178_11555"
     CDS             21374..>21763
                     /locus_tag="VN178_11555"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWS81648.1"
                     /translation="MLSDIEIAQRAEPRRIVDVAWEKLGIGEEHLDPYGHYKAKLSLD
                     FIRSLEDRPDGELILVSGISPTPPGEGKTTTTVGLGDALNRIGERAITCIREPSLGPV
                     FGIKGGAAGGGHAQVIPMEDINLHFTGD"
BASE COUNT         3942 a         6757 c         6981 g         4083 t
ORIGIN      
        1 agtccacgcc tgccggcgcc cgccctacgg acaccccgct atcgaccgac ttcgtctctc
       61 tggtcccgca cgacgcgaag gccagaacga ggagcacgag cacgctcacc cctaaagacc
      121 tcgccaccgc tgaaaaacgt ctggctctca tggcatccac ctcactcgga cgtgtacctt
      181 ccgcggagca acttcccgct ccgaagggcc catatacggc ccaacctact ccctgtacac
      241 ctccagccct ccatcggttc cccgatttca acgaaccgat tcggcgatgc ggatcgcctg
      301 ctccttatgg aggtctgctt cgagtcggag ggcgtacccc cctttctccc agagcagtac
      361 gccgccgggg agacgcgggt ccatggccga gggaatgcgt ccagcagatc cccagtatcc
      421 gggatctccg tccacgctca cccgttctag ctccgatccg acagtgctct ttcctccgag
      481 atacgccgac tcgacgtcgc ctggcgtctc tgtcaggacg aggctgatcc cggcgcctcc
      541 gaggggcggc aaaccgtccc tatagaccag cacgacgcca ttcttttcgg aggttccggc
      601 cgcgtagact tcatcaggct tacctagatt cggtatccgg ggtaggagga gcgcgccttc
      661 catcctggcc cgcgcctctt gcagcgtaat tctctccccc gagaaccgtg cagccagcga
      721 ctccccactc ttggacacgc cggcggaggg tgctgctgcc tccgtctgtc tctcgggtga
      781 gccggcatcg acggcgggtt cgccggcatt atggacacct tccgccacga accagtcgcc
      841 tatcgtggcg cgcaggccag gggaaagggt cgggacggcg acgacgagaa cgaacgccgc
      901 cgctaccgcc acccaacgca tggccgggaa agcagtaagg aaccttcggg gccggctgtt
      961 ctcttcgtcg agcactctgc gggcggcgcg cgcggcgtcc ggcgtcgggg gataatcgat
     1021 gaagcccccg agctccttca gctctctctc gagttctcta tctggcatct ccctccttcg
     1081 caatctcggt gcgcagactc ttgagtgccc gcgagagccg cgatttgacg gtacctcgcg
     1141 cgcaacccag gatcgcggcc gtctcctctt cggagaggcc caagaagtag cggcagccga
     1201 tagcccaccg cgcctcctcg cgcaggccgc cgagggcctc gagcagctct tctctccgct
     1261 cggccgccag taccgccgcc tcgggggagg cagggttatt ctcaggcgtg gcctcccgcg
     1321 ccgcccgcag gaacagaccg gcgcgacgcg cggccgccct gcgccggttt ctggcctcgt
     1381 tggcgactat cgcgagcagc cagggtctga aacgggcgcc ggatcggaag cgtcccaggg
     1441 cgcggtatgc cttcacgaag gcctcctggg tcgcttcctc ggcctccgag gcgtcgcccg
     1501 tgatgaggta ggcggtacgg aaggccacct cctggtagcc acgcaccagc ctctcatacg
     1561 ccgcagtatc tccgcccttc gcccgctcgg cgagcttcgc gtcatccgag gtgacggccc
     1621 tcctcctcga cgttgctccc tactaccatc tacaacgccg ggcgcgaaaa ggttcccggc
     1681 atgatggcta atgatgacgc ctcaggcgag tctctgcgag cgtccacccc gagaaatccg
     1741 catttccctg cgtgctagaa tgcctgccca tgcaggacga acgactcggg gaaaatacca
     1801 cggcggggaa gatagaggga ctggggcatc tgcgtgaggc cgcggcccac cctgcccccg
     1861 agcaggccgt cgagaggcag cgcgaacgcg gcaagctgac ggcccgcgag cgcgtcgagc
     1921 tcttgctcga ccccggcacg ttcgtcgagt tggaccgcta cagggtgcac cgctcttaca
     1981 acttcggcct ggaggagaac aggcccctcg gggacggggt catcacgggc tacggcgagg
     2041 tctccgggcg caaggtctgc gtcttttccc aggacttcac cgtgttcggg ggttccctgg
     2101 gcgaagtctg cgcgcagaag atctgcaagg tcatggacct cgccatctcg acgggctgtc
     2161 ccattatcgg gatcaacgac tcgggcgggg cgcgcatcca ggagggcgtc gtctccctgg
     2221 ccggctacgc ggacatcttc caccgcaacg tgctcgcctc gggcgtggtg ccccagattt
     2281 ccgtcatcgt cggaccctgc gcgggtggcg ctgtgtactc gccggcgatc acggacttcg
     2341 tgttcatggt cgaggagacg agccacatgt tcataaccgg ccccgacgtc ataaagagcg
     2401 tcaccggcga ggacgtctcc ttcgaggacc tgggcggcgc ggccacccac aataccaggt
     2461 caggcgtcgc ccacttctcg gcgccagacg aggagacctg cttcgacgac gtgcgctacc
     2521 tgctctcctt tctccccgag aacaacctgg agagcgcccc ttacttcagg ccgaccgacg
     2581 acccggagcg gatggacgag gacctggcgc gtctgatccc cgactccccc cagcgcccct
     2641 acgacatccg cggcgcgata gaaggcgtaa tcgacgacgg ggagttcttc gaggttcagg
     2701 aaggctgggc ccagaacctg gtcgtcggct tcgccaggct cgacggacgg tccatcggcg
     2761 tcgtagggaa ccaaccttcc gtgcttgcag ggacgctcga tatcaacgcg agcgtgaagg
     2821 gcgccaggtt cgtccgtttc tgcgacgcct tcaatatccc gctacttacg ttcgttgacg
     2881 tgccaggttt tctgccaggg acagaccagg agtggggtgg aatcatccgt cacggggcga
     2941 agctgctata tgcattctcg gaggccacgg tgcccaagat gacggtcatc accaggaagg
     3001 cttacggcgg ggcctacgac gtcatgtgct ccaagcacat cggggccgac gttaacgtct
     3061 cctggcccac gggtgagatc gcggtgatgg gtgcctcggc cgcagtcggc gttatcttca
     3121 ggcgccagat ctcggaggcc gaagacccgg atgcgagacg cgaagagctc atcgaggact
     3181 atgaagagca gttcaacaat cccatggtcg ccgcagagat gggttttatt gacgacgtca
     3241 tagacccgcg cgagacgcgg ccgtacctga tccaggccct ccagatgatc cgcaccaagc
     3301 gtcccgacag gccgccccgc aagcacggaa acattccgct ttgatagctt tcggctttca
     3361 gctttcggct ttcagcttga tcactttggg tcgccggtag gtggagaatg cctgacgacg
     3421 acaggaagac gttaggatct cctttcccct taaggattca gccggcgccg aacctggagg
     3481 aggtcgccgc tatagttgcc gctctggaga cgctctccgg aggagggcaa cgagacagcg
     3541 cttccttgaa gcgcagccgg tggcggctgg ccggccttct cgggcacgca ctagaacctg
     3601 ggatagagcc ggatggctcc ctgtgggcct actccaggtg ggagggctag ctccggtgtt
     3661 cggtaaggtg ctgatcgcca atcggggtga gatctccgtc cgcatcattc gcgcgttgcg
     3721 cgagatgggg gtaaggagcg tcgccgtcta ctcggatccc gacagggaag ccctgcacgc
     3781 cagcctggcg gacgaggcct accacgtcgg cccggcgcca gcagcggtga gctacctgaa
     3841 caaagagagg ctgatcgagg tcgcacgaaa gagcggggca gaggccatac atcctggata
     3901 cggcttcctg gccgagagcg cccctttcgc gcgcgctgtc gaaggggccg ggctagtctg
     3961 gatcggccct catcctggtg ccatagaggc gatggggagc aaggtggaga gccgaaggat
     4021 catggctgag gccggtgtcc cgatggtgcc tggcacgcga gactccatca ccttcgctaa
     4081 gcagatcagc ctcttcggcg aggagcacgg cttccccgtg gccgtgaagg cctctgcggg
     4141 cggcggcggc aaaggcttcg ccgtggccag agacgcctct gagtctgcga gcgccttcga
     4201 aagggcgagt agggagggcg aagcttactt cggggacggt tcggtctaca tcgagaagta
     4261 cctttccgcc ccgcgccacg tggagataca ggtcgtgcgg gacaggcacg gtaacgcggt
     4321 ccacctgggt gagagggact gttcgatcca acgccgccac cagaagctcg tcgaagagtg
     4381 cccctcccct gccatagacc cgatgatgcg cgatgctatg ggcaaagccg ccctcgccgc
     4441 cgccgaagcc gtcgattacg acaacgttgg caccgtcgag ttcctcctcg aagagggtga
     4501 agccacaggc gcgttctact tcctggagat gaacacccgc gtccaggttg agcatccggt
     4561 gaccgaagaa gtcacgggga tagatatcgt gaagaccggc atccgaatag ccgccggcga
     4621 ggaactcccc ttctcgcagc gcgatatctc ctggcgcggc cactccatcg aggttcgcct
     4681 gaacgccgag gacgcatcta acgacttcgc cccgagtcct ggcgtcgtta cggcctatgt
     4741 ggagccaagc ggtccgggag taagggtaga ctcctcgctg cgcggacctg gcgtcgtggc
     4801 tgagtcctac gacccactgt tcgccaagct catcgttcgc ggttccgata gagaggaggc
     4861 cttggcaagg ctcgcgcggg cgctcgccga gttcagggtg gaggggatct ccacgacgtt
     4921 gcccttctac cgtgcgatcc tggacgatga ggtcttcgtc tccggcgact acacgacggg
     4981 ctttatagca gaacggatgc atcggctaga gatacagcct gctgtcaccg gagaaaccgg
     5041 cacgtcccct gagaagcacg cccgtgaggt cgaggtcgag gtgaacggca agctcttcag
     5101 ggtgagggtg ttcggggatg agggcacgac cgtcgggccc ccgcggcgca ggcgtggcga
     5161 gacgcgtcgg gcttcgacga acgagggtgc gatccgggcg ccgatgcagg gcaccatcgt
     5221 aaaggtgctg gtggaggagg ggcaagaggt cgccgcggac gaggccgtgt gcgtcctcga
     5281 ggcgatgaag atggagagcg aggtccgctc ccagaaggcc ggcaccgtct ccgaggtccg
     5341 cgtagaggcc gggcagaccg taagatcagg ggacatcctg atctctgtag agtagggcgt
     5401 ccattgaccg aacacaccgc agagtacgag tggcacggcg acggtgagga aggcgaggtc
     5461 gtcctctacg ccccggacga ctcggctttc gaacgcgcct tgcccgccgc tagattaccc
     5521 ggcgtggaga gtcccgtcta cgccgcggcc tcgcaaggag actttggatg ggccgcagcc
     5581 tcagccacgc acgcagcccc cgatctgctc tccggccctc tccgcggtct gctcctcgtc
     5641 gccggcacca ggtctgagaa cctcggtgta ccgccacagg agctgagaga cagaatacgg
     5701 cgtgaactcc ccgaggccgg cgcacgccta ccttccttga acgagtcagg ggcgggcagg
     5761 ttctgcgagt cgggggctcg ggccgcggcg gaggatgacc tgatcgaaga agacgatcta
     5821 ccgcttctgg atgtgtgccc tggcgacgcc gacgccttag gacgccgggc tctagccgcc
     5881 gctccgcgcg catgggcggg tagggtggaa ccggatctca ggttggtgac ggaggtcctc
     5941 gacaccgaag gcgcggagag cttaggactg gaggaaggag ccctcgccat cgccgtcaag
     6001 gtcgacgcag gagacctcgg ccggctcgcg ctcgccgccc acaaggagcg tattttgagc
     6061 cgtacacaat cgggagattt tggctctacg cccgaactcc cggctgcccc tgcctcaagc
     6121 ggtgaggtcg aggacctcgt cgcggcgctc tacgcagcga ccaacttcgc ggacgcccgc
     6181 gccgcaagga ctgtgtggat gctgaggcgg gtactgggcg acgcggcggg agggctggag
     6241 atcctcgccg cctggaggac cggtgggatc gaggagcagg aagggcgcct ggtgcaccga
     6301 aggggtttcg cttgggccga gaatggacat gtgctcgtct gcggaagtta cgcggcggcc
     6361 ggaacgggta atatgtgggc gagtgcgccg ccgttcgggg ttccggagga ggaaggccgg
     6421 tggccgtggg aagaggctgg gctgttggaa cgtctggtga cgttggatcc cccggagggc
     6481 tgaggttgga cctgttgatg gttagggacc ggagcacggg ccggttcgtc tatacggagc
     6541 gactggagcg tagatccggc gagacgtcct gggaatacgt ccgtcgtagc gtcaggaggg
     6601 aggcccgcat ccggacgcgc ttcgacgggg acacaatgga agtcatcgtc gggtgggatg
     6661 tcgattcggt cgaagcgttc ttgcgcgcat accccgagta ccgcacggac ggggactccg
     6721 acgcagcgtc cggaagaagt agagagcacg aggaacgtac ggaaggagat gccgttgacc
     6781 ggtgaggttt tggacaggtt ctctgagctt gtaacgaccg ccctggggct cgtggccgca
     6841 ctggcctgga acaccgccat ccagaacctg ttcgacacag ttttcggcgg gtcaggcagc
     6901 aagctcgccg gacagttcct ctacgccacc ctgataacct tagtcgtcat cttcgcggca
     6961 atagccgtaa gtcgtgcgca gaaagagcga aacaggccga ggaggaacga agagggtaga
     7021 gggcggttcg tggaccggga tagggaccgt caggcggcgg gggcgggttc gaaacccgcc
     7081 cttacttgaa atcttcgggg gagacctggt tcatccagtc cttgaattta tccacgacct
     7141 cgtcctctgg ggtgtcttct atcgcctcct cgaagacgac gccggcctcc tctatcacct
     7201 cgtctgaggc gaagatctcg gcccccgatc ggatagcgag ggctatggcg tcggacggcc
     7261 tggagtcgac ctccaccgtc ttgccttcta tctcgagcct tatggtggcg aagaaggtcg
     7321 agtccctcag ttcggtcacc gtgatcctct ccatcgatcc gccgagttcg ttgaccaggc
     7381 tcagggctag gtcgtgtgtc aggggcctga cgaactcctg gttctgaagc ttgaacaaga
     7441 tagagcgggc ctccgcctgg ccgatccaga tcggcaaata gcggttctcg tcttcgacct
     7501 tgaggataac tatcgggctg gaggaaaaga ggtccatgtt catgccgtag atagacatct
     7561 tggtaaagcc gttggcgctc actggctatg tgctcctctc actccgcgag gtcgcggggt
     7621 tctcttgtac tggtcgattt tactgccagc ttcttctgtg ttcaatcagg ttgtcagtag
     7681 cctgcccgtg ttgtacatta ttccggcggc gaagatccgt tgcgccgatc actcagggcc
     7741 tgtagctcag ccggttagag cgcttctctg ataaggaaga ggtccctggt tcgagtccag
     7801 gcaggcccac tgagatatcc ctttatctaa gccatttctc tctgcgctgc gcctcttcga
     7861 tggtctgaat tgtgccgtag cgtacggtga cgtctcgtgg ggtggctgtc ggtggccctc
     7921 aacgggagag aaaccagtcc agcagatcgt agacgtagta ggcgagggtg ccgtcggtaa
     7981 gagggttgcg ctcgaactcc ccagcggcag cctttttgcg gtaagtcgat ggcggcatct
     8041 tgaggaatgc agcggtccgt agccaactta tgagagtagg actctattct tgcttctccc
     8101 ctcattggtc tctgctctta cacggtagct caagccgagg accaggacac cctacggcga
     8161 gcgtgtgcgc gtctagatta tggtgcgtct cgcgccacac gatccgcctg tcgtctacat
     8221 aatctgatac gcaataggcg cgcccctcgt tcattccgca cgctttagcc catgctgggg
     8281 aggtccgagg atccccgcgg tcgcccatgt acgcgcctat cctggagtcg ggctttacgc
     8341 cgtcggcgat gaaagcttta tagtggtcag gaccgtcgca gtcactttct agaaagtcgt
     8401 agttccaaca gctcgttatg ggtgcgctat cggtgaaggc tccgcctttg accgggtgca
     8461 catggcgggt gtgatcgtgg ccgaagaacg acagcccgaa atgcccgagt gcgggttcca
     8521 agaacatggg atgccaatgc ttcattgttt gggtatctct tgctgcataa ccggcggacg
     8581 caagtagcgc tacgaagagg cacgagcaga tcagcaacgt cgtcaaccgt ttcaccattc
     8641 gtttacccga aaggcagttt agcaccttgg acgcggcacg cttggtcata cactacgccg
     8701 ctgagacctc cgactttgac tgagctacgg ctcgcgtcaa aagagaggtc actgacctcc
     8761 agcgtgatat gggcaaacgg cgtcccgggg agcttaagcg tttggtgtga ggtgtgagat
     8821 caccatccca acaagtcagc gaaggctagc agggggcgac cgagttgccg ttgagccagg
     8881 tgcagaagtt gtgcgagtct atcctcgccg ccgaacgcgc ccgtaaccca ctcagctcgt
     8941 gaggccgctc tcgccagacg ccggaggtat tggacttctg gcgactactt cccgcactcc
     9001 gtcagcgcct ggtcgatgat ggaaaccccc tcatcgatct cgtcggtggt caccgttagg
     9061 ggcggtgcga ggcggaagac gttgcccaac cccttgaact tcacaatgtt catgttgagg
     9121 ccgagctcca ggcaccgccg cgttacccgg gcgccgagtt tctcatccgg tgccctgcct
     9181 tcacggtcct tgacgagctc gacgccgagc atcagaccgc gtccccgcac gtcgccgatc
     9241 ggctcgtggc gggcctgaag ctccagcagc gccgagcgca gccggtcccc cattaggcga
     9301 gcccgctcgg ccaaacgttc ctcggcgagg acccgtagca cagccaggcc gacctcggcg
     9361 gggaggggat cggagacgtg ggaggtgaag tgcaggaagc ccttctcgta gcactcctcc
     9421 tcgatcgcga cgctggttac cgtcgcggag agcgggagcc caccgcccaa cgtcttggag
     9481 agggtcagga cgtcggggac gacgtcctcg gcctcgaagg cgaagtcggc gccgacccgg
     9541 ccgagcgccg tctgcgcctc gtccaggatc agcaacatgc ctcgctcctc gcaggcccgc
     9601 ttgaggcggg cgaggtagcc tttcgggagc gggacgatgc cgcctacgct caggacgggc
     9661 tctgcgatca cggcagcgta cgcgcccacc gactgggagt ccgcgagccc gacgccgacc
     9721 tcgaggcagg tcatgtcgca gcggtcctgg cagtgccgga tcgggcaccg gtagcagttc
     9781 ggagccggca ggaccataga acccggcatg gcggggccgt accctttgtg gccggccgag
     9841 tatgtgctgg acgaggcgcc ggcggtcatg ccgtgccagg agcccgtgaa cccgaggacc
     9901 tcgaatcccc cggtcttcag cttggcgagg cgcagcgccg cctcgttcga ctcgccgccc
     9961 gtgctcagca acagcacctt ctgcagcgcg ggcggtagca tcccggccag ctcccgcgcc
    10021 agcaggaaga ccggcggcgc gagcatgccg ctaaagaggt gaatggcttc ccggcaggcc
    10081 ttctccatcg ccgccacgat ggccgggtgg ttgtggccaa gggtcgcgca catctgcccc
    10141 gaggtgaagt ccaggatact gcgaccgtcg acggtgtaga ggcgggagcc ggaggcgctg
    10201 gagaccagct ccggcacgaa atcaccgccg taccttacga ggtgggcgtc gaccgcgtcc
    10261 caaagctcgc gcgagtctac ctgcgtgtcc gcctgcatgc tcctcgtcga ttccatgaat
    10321 cgacctcctc ttgcggggta ccgaccggac ggcgcaccgt ggtagaagga tagagtgtcg
    10381 ccactgaagc gtcaataaaa tgacgtgcca ataacatccg acgtttgaga ccctcgtcat
    10441 cgaggggctg ctccgcccaa agccccggcc ctaccgggcc ggacgtccag gcgggccagc
    10501 tggtcggtgc ggctgcgctc ccgttcgtag accgcgacgc cgcccttcct cagtccctgg
    10561 gcgaggcata gaccacgatg acgtggaacg gtgaaccaga gattacgtgt acctcccgat
    10621 cttgctctgc gcttagatta gagtctttgc acagcgttct atttaggacc aagcctcgcc
    10681 taacttaggg gcgggggttc gtatccaacg atcacgtctc cgagcagctc ggcgaacgtg
    10741 atcgccgtgt cgtcttcgta aagcggcccc aggatctgcg cggcgacggg gagtccgcct
    10801 ggcgtgcgtc ctatgggcgc ggctacggcg ggcaggcccg gcaacgaggc gtgcgaaacc
    10861 cagaaggact ggtcgctgta ggaccgctcg ccttcgggcg tcgcgatcgt gcgctcttcg
    10921 aagggccttt cgtcgtgcgg gaaggccggt gtgaagctta ccgggcacaa gaacacgtcc
    10981 atgtcgtcga agtagcggtt ccaggcggct cgcgaggcca tgcggcggtt ctcgtggtcg
    11041 acgaactccg ggaacgtcgc aaagtcttcg ccgggttgct ggaaggcgaa gaacgcctgc
    11101 acgtggaacc cgaacgattc gtagtgctgc acagggtcca ctccgtcggg ccagccctcg
    11161 acgaccgacg cgccggctcg ggcgagcgcg tccacggcgt tggacagcgg tgtggcgacc
    11221 tcgccggaga cgtgggcacg ctcgtggtcg agcacgacgc ccacacgaaa atcctccagc
    11281 cgggcgtggc gcggtggcga cagtgtccac gagtaagccc tcgccgcctg cttctcgggc
    11341 cctgcggtga cgctcaatgc tgttctgagg tcgcgtggag accgggcgag cggtcccacc
    11401 gctgacatgt acgtcatctc gctcggagga ggtgggggac caggaggatg gaacccaccg
    11461 agcgggacga tcccggcgct cggcttcagc ccgtagacgc cgcagaagct cgccgggatg
    11521 cgaatggagc ccacgagatc cgatccgtat tcgaggaaag tcatcccggc agatagggcc
    11581 gcgaccgccc cgccggagga gccgccgggc gtgcgcgtcg tgtcccacgg gttgttggtg
    11641 acgccgtaga gctcgttggc cgtctgcccg aagtcgccga gcatgaaggc cacgttggtc
    11701 ttcccgacga tgatcgcgcc ggcctccttc aagcgccgcg cgaccgtggc gtccgagccc
    11761 gccacgaagc cctcgaaggc gggattgccc caggtggtgt gcaacccggc gacgttgaag
    11821 ccgtctttga tcgtcatggg cacgccgtgc agcggtccgg tggcgtcgcc actgtcggtt
    11881 gcttcgtcgg cggcgaccgc ttcccgcaat gcggcctcgc gccggagctc tacaacggcg
    11941 ttcagcgcgg ggttgacggc ttcgatccgc ttgatgagcg tcgcggtgag ctcccgggag
    12001 gagatctcct tgcgacgaac cacctgtgca gcgtcggtcg ccgaccggaa cagcagtgtc
    12061 tggtgtgtct tcttcgcctc cgtttgcatc ttcttctcct ttcagcgcgc gacgatgggt
    12121 tcctggagtt ccgtcaccca cgcatccctg ttttcgccca gctcgatgta gagctcgcgg
    12181 ttgtaaccga cgggacggta gccgttggcg tcgatccagc gggccagcgc ctggatggtc
    12241 ggcatgacgt tgtccatgga gccgcggtgc aagatggtgg ccgcctgctc gatctcgggc
    12301 aggttcacga cctggaagtc gtggtcctcg ccgggaccga cgttcaccgg aatggtggcg
    12361 tggatgagga ccccgtctcc atccggggaa tcttcgtagt aggcaaggtc cggaccggcg
    12421 ggggtcaacc cggcacgttc gagccggccg cgcagctcgt cgtagagcgg cccgatgacc
    12481 ggagcgatgg actcgggttc gaagcccgcc gcggtgccgg taagctccgc gacccggagc
    12541 cccggaatcc gcttgatctg gacctcgtcg gccggcatgg cgccctccct ttcgatgatc
    12601 cggagcctcg cctctacccg cgccaaccgg gcggtgtcgg tctcgatctg cgattgcagc
    12661 tcggcgtgac gcagccgcaa catcccgcgc agctcttcga cgctcacctc gtcgtctagg
    12721 acggatcgca cctgctggag ggtgaagccc aggcccttca gcgcgacgat ccggttcagt
    12781 cgcgacagtt gccgggcttc gtaggagcgg tatccggtaa cccgatcgac gaacgccggt
    12841 ttcagcagcc cgatcgcgtc gtagtgtcgc agcatgcgta ccgaaacgcg gccgtgtctg
    12901 gcgaaatctc cgatgctgag catgacgttt ccaagtctac ggcctgacac ggtgtcaggg
    12961 tcaagacccg atcaggcgac tttcgaagaa ccagggctta gcgttcgcgt tgctcaccac
    13021 aggctccatc aagtgggaac ggtgtggtgg accgcctcga cgttgttgcc atcggtgtcg
    13081 aacgcgtagg ccccgtagta gcccgtgtgg tactcgggcc tcagactcgg cgccgtggcg
    13141 cggaaacgct tcgatcgcct cccggccggg tagcggcgga attggtcaga gatcgccact
    13201 tggaaaccat gcaaggcggc tttgaattac cttggtcggt aagatgtcgc cggatacgca
    13261 agatcgtcac cgtatgatga gcgacagaga gaggagcctg accgtgttct tggaactgcc
    13321 agagagggtc gcgatcgtca ccgggggcgc acggggcata ggattcgcca tcgccgagcg
    13381 cctctcgcgg gctggcgccc gtgtggtgat cgcggacatc gacgaagaaa gcgccgttgc
    13441 ggccgcggaa aggctgcgcg atgggggagc agaggccgtg gaagcggtcg cggatatcac
    13501 caacccaggc gaggccagtg cgatggtcga ccgtgccatc cacgctttcg gcaagctcga
    13561 cgtactggtg aacaacgctg gcataaccgg ccgcgacgcc tcgctctctg aggtcaccga
    13621 cgaggattgg gagagggtgc ttaagctgaa cctgaccgcc gccttctatt gttgccgggc
    13681 tgcgatcccg cgcatgcgcg agcagcggag cggagctgta gtcaacgtgg cctccatctc
    13741 cggcaaggaa ggcaacccga acatgatccc ctactcggtc tccaaggcag ggttgatctg
    13801 cctgaccaag gcgttggcca aggaggtgat ccacgacggt gtgagggtga actgcgtggc
    13861 gcctgccgtc atcgagacgc cgctgctcgg ccagctccga ccggaggcca tcgagtacat
    13921 gacgagtaag atcccgatgg ggcggatggg gcgagcggag gaggtggctg cggtggtgca
    13981 ctttctggcc tcagatgacg cgagtttcgt cacgggccag tgctacgacg tcagcggtgg
    14041 tagggcgacg tactagcgag gtggccgtag ctgtaggttc ggccgcctcc cgaacgaacg
    14101 agcctagcaa ttgatccgtc gtctcgacac gggaagggac taccatcccg cgctaagcta
    14161 cagggcagga tgaatagaat aggtaagcgc agtatctccg agcaggcgat ggtcaggaaa
    14221 ggagaacgaa cgtggggaaa gtctgcacgg ggcactcgac gtcgctggac ggctttatcg
    14281 ctgggcccga cgacggccct gaagcaccca tggggctcgg tggcgagcga ctcttgggat
    14341 ggtacgccgc cggcgacacc gaatacaggc tgcccggtac cgagatggtg ttcaaggtct
    14401 cggcgcagag cgccgagctc cttcgcgaga cgcgcagaac gacaggagcg ttggtgacgg
    14461 gacgcaggac gttcgacctc acccgtgggt ggggtggcag acaccccttg gacgtaccgg
    14521 tctttgttgt tagccatacg gtcccacaag aagagtgggt tcacgaagga tcgccgttca
    14581 cgttcgtcac ggacggcctc gaaagcgcca tagagcgagc gaaagcgatc gcaggcgaca
    14641 aggacgtcgg cgtgatcggc gcgagcctcg tgcagcagtg catcagagcg ggactcctcg
    14701 acgagataca cctcgatctg gtgcccgtcc tgctggggga cggcgtccgg ttgttagact
    14761 acccaggaac cgagccgatc gagctggaga gcacccgggt gatcgaggga gctggggtca
    14821 cgcatctcac gttccgggtc gtaaagtgag caaccagacc taggactttt gggacgtctg
    14881 cacggtcgga atctgtggct tgccgcctgg tcggggtgct aaggctcctt ccagactctc
    14941 tctacttgcc cttctttacc cgagtgtgga gaaacagtct gacaaaggga ctgagcgacg
    15001 ctccgagcat ggatttggcc ggtatatcgg ggccaaaact ggccgaacag tacggtgtcg
    15061 gtgctcagtg cggctccgtt tcgggtgcct acaggacatt tgggacagtc tcggaagttg
    15121 cgcctgttct tgtaacccat cagacttggc tcaattgttc aacgtggcgg ggttagcata
    15181 gagtagtccc gcccctcttt actgaggagg agttcagaga aatggtgttg agcacaagat
    15241 tgagggtcct cttggtggtg ctagtgatgg cgcttggcgc agtaggtgtc ttgggggccg
    15301 taggctccgc ctcgcccaac aatagcgaag acgcccacca tcgcgacgac aaagggcgta
    15361 ctaccctaac cgtcgtggga aagaaccccg agaacgaact cttagacctc ggagcacaag
    15421 gaccaagcca gggcgatcta cgcgtcgcga acgccccact ctacgacgag agcggaaagg
    15481 agaggatcgg gcgcttcgat ctgttctgcg caataaccga tcccgccgat gagccctcag
    15541 agaaggccca catggcccag tgtaccaaga cgttcactct gccggccggt cagatcagcg
    15601 tacagggcgt agaagcctac cccgatctct ccgggctcgc accaggggtg aacgccataa
    15661 gcggcgggac gggcgagtac gctggtgtgg agggcgaaca acgattcgag gtgcgcggaa
    15721 agaaggtcat cgacaccttc cacttcatcg actagtgttg gaccagtctt aagctagaaa
    15781 aggggaaggc cgggccttcc tcccttgagt tggaggggtt gatcgctccc cggtgtcggt
    15841 tgcgaatagc agcccccttc cggagccggc cggtttcgat cggcaaccct cctcccacta
    15901 cagcgaacat gtcgctaagt aagggtagct tcggcgggcc tggccgggtc ttcgacagtt
    15961 gcttcaaagg cgccccggcg gtctcttcgg tcatctctgc gcccttgccg gcttcggcga
    16021 catctccggc attttccagc gccctgaatc ccggggtgtc ctccagcctg gttctaaggt
    16081 agagcccgac gatgcccagc ggcagggcga ttggcggcgg ttccacccac gatcttgccg
    16141 aagttgatca ccagcggcgt ccttggatcc catcctgcgg gacacgatct tttgtgactc
    16201 ctggataatc gccgcgagcg tggcgatcgc gtcgcgaccg atgttggtgg agagatcctt
    16261 ccccgaagat aatctgccag gcgtttgtcc tgcacgtgcc tcatgacgac ctacaaggtg
    16321 ttgaccactt ccctgatggc cccggacatg aactccaggt cgtcctcggt tacgatcagg
    16381 ggcggcgaaa gggtgatgtc gttgttgaac ccggcggtcg tatcgccggt cttgcccacg
    16441 atcaggccgc gttctttaca ccgggagaac acctcggtgg tcagcccctc cgcaggtttc
    16501 ctggtctcct tgtcctcgac gagctcgatg cccagaagga gacccttgtg gcggatctca
    16561 ccgacgttgg gatggtcttc gagatcggag agcttctcgc caagcttttc gcccatctgc
    16621 gccgagcgct cggggagccc ttcgtcctcc atgatctcca ggttgcgcaa ggccagggcg
    16681 caggaggtgg tgttgccgcc gaaagtgttg acgtggcgga agtgatcgta ctcctccgtg
    16741 cccttgaacg cctcgaggta ctcgcgcttg atggcggtgg ccgagagcgg caggtacccg
    16801 ctggtgatcg ccttcgccat cgtgacgatg tcgggcttca cgccgtagtg ctggtggccg
    16861 aacttctttc cggtcctacc aaagccgcat atgacctcgt ccatgatcag cagtgctcca
    16921 ctctcgcggc agatctcggc gacgttctcg acgtagcctt ccggcggcaa gatgatgcct
    16981 ccaccggtga ttatgggctc catgatcacg ccagccaccg actcggcgat ctcccagttg
    17041 atggtgcgct cgatctccct ggcacactcc atggtgcagg cgggccggtc ggcgcaatat
    17101 ccgcaccggt agcgcatcgg cggctggacg tggaggaaac cgggcgacaa cggctcgtag
    17161 cggtacttgc gctggcccat acccgtggcc gcgagggccc cgcctcccgt accatggtag
    17221 gcgcgatagc gggctatgaa cttgtgccga ctcccctcac ccctctgcaa gtggtactgg
    17281 cgggtcatct tgaatgcgac ctcgttggcc tccgacccgg agttggagaa gaagaacacg
    17341 tactcgtcgt cgagccactc gttgagcttc tcgccgagtc tcacagccgg ctcgtggctc
    17401 aaggtgagcg ggtagttggg cagcttctgg agttgctcgt atgcagctct ggctagctcc
    17461 tcacgaccgt agccaacgtt ggcgtaccag agccccgcca tcccgtccag gtagcggttg
    17521 ccttccgtgt ccgtgatcca ggccccatcg ccctccgcca ccatcatcgg tgcgcccggc
    17581 tgcgacaatc gcgccatcgg gtgccagacg tagcgccgat ccatctccgc cagcttgcta
    17641 acccgcgtat ccttcgcctc cactaaagat actcctcctc ttctgatcgt agccccacca
    17701 accaactctt acatgttttc gactcgtcca ctactttccc ccggctcctc caagccgtat
    17761 gatcctcacc taaccacttt gtgtagcata tcgtatattt gacctgtaga ttttgtcaag
    17821 cgtttctaaa ttaagacttg taaatctcta tagaaccgca ggtgtttggc tggcgaaaat
    17881 gtactcgccc ggaccttgac gggttctgtg ctatctgtct ccgggtgtac ttctctagtt
    17941 cagaaggtag gaggactatt gacgtagatg acttccgctt cttcgtcctc cgtccaccac
    18001 cgatggggtg acgtagaggg gaaatagagg acatccctgg cttcgaggcg gaatgtttcc
    18061 cgctcctcga gctccacgta gaaggagcct gagaggacgt acacgaactc ttcgccatcg
    18121 tgcgagtaga atcctccgct gcccgagtgg ggcggtacgt aaacgaagga ggggttcata
    18181 agggacccac tggcggccaa ctcctcgtag cgaacaccgt tggaccattg catagtcggc
    18241 ctgtcttcag gccggacgag gagagaactc tttttcagat cagcatcgaa gacgtcccgc
    18301 atattcatcc cgtagacctt gactatagaa ctcaaagaag cgatggaggc gccggtctcc
    18361 ccgcgttcca gggcggagag gaatgacggt gacaaaccgg tggttctggc cagctccctg
    18421 atggtcatcc tgtgtcgcgt gcgcagtagg cgcaggcgca ggcccactcc tgcagccgcc
    18481 ggttggtcat cctcgcggcc ttcttggggc aatgtcgaac tccccgtgga ttcgagctcc
    18541 ctgcggattg cagccgaatt caggcggtgt atggtcttga gctgctttat cctcttgagt
    18601 ctctcgacgt cttccttgct gtagtatctg gtaccgcctt tgcttcgcga cgggcttacc
    18661 agtccctccc tctcccacac acgcagcgtc cctggcgcca cccccacagc ccgagaagcc
    18721 tcccctatag cgaacctgta agacccgttc tccatcctcg acctaccagt gatccatcaa
    18781 gcacgagtaa gatccctgtt caggatctcc atcccaatta tagccccgac acaccgcggc
    18841 cgtgtcggcc aatctcagag gctgaccgat gaatttgtac tacccttgta gatttgaggt
    18901 acgggtgtct cgtcgggtga aaacttcatg cctcgatcgc cgtcagtggc ttgcacggta
    18961 tggatgcatg ggaggaagaa aggatgagcg agaagatacc tcccggccaa gctactcgcc
    19021 gtcggggtag ccgaacgtgt aggcgatccc ggcgttcgtg gtggcgatcg ccggaaaacc
    19081 gcaatcctcg aataccctcg cgctcatgcc atcccaggcg ttgggcagga cgagtgtgtc
    19141 accctcgcgg tataattctc cacgtcgctg cgccttcgac ctctgcgtcg aagcactcac
    19201 gttatctcct atatgtcatc ttctccaggt cggtccgcac cggaagcacg gtagtcccgg
    19261 cttccccgcc acaggtcctg ggaaggcacc cgcaaagccc ggttgaactt gctcgaggaa
    19321 ttctcccagg cgatgacggc ggtgagctcg accagagcat cgtcgtcgaa aaacgcccgc
    19381 acccgctcga atagttcctc gccaacctcc cggtcggaga gcgtgatggc gtcggcgtac
    19441 tcgagcgcgg cccgctccat ctcgccatag agagggctct cagcgtaccc ttcgagggcc
    19501 acgatcttct cgtccgagat ccccagttcg ctgcccacgg cagcgtttat gtccgctcag
    19561 aattcgcagc cgttgagcgc ggcgacccta cggttcacga gggccaccag ccttggatcc
    19621 agcagtcccg actgctccag tcctccccac atcccccgca ctccgcgaaa tatggtcggc
    19681 ctgcgagcgt agagcagatg gttcgatagc ggcccacccc acttctccgc ctgggcctcc
    19741 agaacagccc tgatccgctt ctcggccctc tccgggtcca ccccttcgat gcgcgccacg
    19801 tactctagcc tcctctcgtc cctgttctca accagtcgcc gcccgtactc acggtacatt
    19861 cggtgacctc cgtgatcccg gcaaactttg caaagctccg actttgcgat aacatatcag
    19921 tgatcaatga tattttcaag cagagacggg tattgaagga ttcggcaaac gtagccagga
    19981 ttgcgtgggc gctctcggac ccggtgcggc tctcggtgat gcaggtcttg atgggcggac
    20041 cggcgacggt gtcggagctg gtttcggcga cgggcgcgtc gcagcccaac gcctccaacc
    20101 acctgaaggt attgagggaa cagggattgg tgaggagcga gcggcggggg aggcaggtga
    20161 tctacgagat accggacact tcagtggcgc aactcgtaga gtctctgacg gcggttgcgg
    20221 gtggtcccga ggcgccggtc ttcgggacgg acccgatggc cgaggccagg acttgctacg
    20281 accatctggc cgggaggttg ggggttggga tctacggtgc tctggtggac tctgacgccc
    20341 tggtaccgac cgcggaggtg cgcggcgacc tgcggcttgg tcccgaggcg acgcgggtat
    20401 tcggggccat cggcgtcgac cccgccaaga cggcgcagga agccgggcgg cgtcggtttg
    20461 ccttcgcgtg tcccgattgg acggagcgga tgccccacgt gggcggggct ctgggcgcgg
    20521 cgatttgcga gcggttcttc gaggagggat gggtgcgtcg gggcgagaga acgcgggcga
    20581 tcgcgctaac aggcggagga agatccgccg ttcgggagcg tttcgggctc gagatcggag
    20641 agacgaccgg tttcgatcgc gtcggagaag gaggagacac ttgagcgagg aggcgaagaa
    20701 gggcaagccg tggaaggaac tgctggccgg ggcgtgctct gtttggtggc gggatctggt
    20761 acccgctcgt gcccgcgcta agggcgttcg ttcggactaa ccaaccagtc attgacgtag
    20821 agctcgctga cctcgacgga ctcgggaagc tccaggaggt actcttcgtc cttctccagg
    20881 tgcgtcgggc gcagatcatc gccgtgcgcc gcctccatgg cctccgtcga ctcccagtac
    20941 gagatggtgg tgaactccgc gacgtcgccg actatcttcc tgaactgctg catgccgaga
    21001 cagcccggct tcttctcgac ctccaggacg ccgttctcat agttgtagcg gaggtactcg
    21061 tccgcaaact ccttgcgggt tcgcccgaac cacgtccgcg caacgagtat ccttccgtgc
    21121 gttccggacg ccaggacctc ctgcttcccg gcggcattgg tctcgaaatc caacttcgtc
    21181 tcctcgaatc gccaactacg tcgaatcgat gctacgatga ccccgcgatc atgtccaacg
    21241 ctgatcgaga aagatgcccc caccgctgta tggtccacac gccaggcgcc acttgccgcg
    21301 aaactgccgg ggcgctatct tgtttgaccg tcagtagtac aaatttactg taaagagacg
    21361 caggggtgag tagatgttgt cggatataga gatcgcacag cgggcagagc ctaggcgcat
    21421 agtcgatgtg gcttgggaga agctaggaat aggggaggag cacctcgacc cgtacggcca
    21481 ttacaaggcc aagctctcgc tggacttcat caggtcatta gaggataggc ctgatggcga
    21541 gttgatcctg gtcagcggca taagtccgac gcctcctggt gagggaaaga cgaccaccac
    21601 ggtcgggctc ggggatgcac tgaaccgtat aggcgagcgg gcgataacct gcataaggga
    21661 gccctcgctc ggtcccgtct tcggcatcaa gggcggggcc gcgggcggag ggcacgcgca
    21721 ggtcatcccc atggaggata tcaacctgca ctttacgggc gac
//
LOCUS       DATMVF010000222         8280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_405815, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000222 DATMVF010000000
VERSION     DATMVF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405815"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VN178_11560"
     CDS             <1..811
                     /locus_tag="VN178_11560"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81649.1"
                     /translation="RTRNKAEELARAGSARVADSPREVARQSAITITMLPGPPEVEEV
                     VAGERGLLKGAREGSLIVDMSTSSPILAQELARIARKREVGMLDAPVSGADVGAVEGT
                     LSIMVGGEEEHFERAKSLFDVMGEKVVHVGPPGTGQVVKACNQIVVALTIEAVSEALV
                     LGSKAGVDPGKILDVLSGGLAANKVMEVKREKLLSHDFEPGGKVEFHRKDLVIALETG
                     REYEIALPVTSMVDQMFGSLMAAGRGGWDHSSLVTLIEDWAGYRPDRHEQS"
     gene            946..2334
                     /locus_tag="VN178_11565"
     CDS             946..2334
                     /locus_tag="VN178_11565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS81650.1"
                     /translation="MAGEQSSREPTELKKDLGGGLILRRARREDAEAVAAFNARIHHS
                     SGDFDQREPHRGIAAGTRDLMSGDHPTCDASDFTVVEDTKTGFVVSSTCLIGQRFSYE
                     GVEFDAGLPELVGSHPDYRRRGLVREQFEVLHRWSEKRGHLMQAIGGIPYYYRRFGYE
                     MAVYMGGGRRIYVQDVPDKPSSSGGAESSPRSYRLRPAAAADAGFLSNLDRRGRGRGL
                     LTASRDEGLWRYEVAGRDPESDESLEMKIVEDATGESVGYVCHAPDLRNGALQVYGYE
                     LADGISWLEATPFVLSELAEFGRKRASGEEELASLTFVLGEQHPLHEAIPEPPLYRLD
                     RQGNYSYYVRVPDLPGFLRHVAPVLERRLAASVAAGHTCELKVSFYGGGIRLELDRGR
                     LSRVEDWSPTVEERGDAAFPDLTFLQLLFGYRSLDELDRAFADCLPGEGDARALLKAL
                     FPRRPSDLWPVF"
     gene            complement(2433..3572)
                     /locus_tag="VN178_11570"
     CDS             complement(2433..3572)
                     /locus_tag="VN178_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWS81651.1"
                     /translation="MNLSSQRDLFEIPEDIVYLNCAYMAPQLRPAREAGEKAVSRKSR
                     PWEITPDDFFEEAEEARALFARLVGGDADGVAVIPSVSYGISVAAANVPVREGEKIVI
                     LEDQFPSNVYAWRELAERSGAKLVTLPRPEDLDWGRALVGEIDADTAVVAVPNCHWTD
                     GSLVDLASVGERVREVGAALVVDAIQSLGAYPFDVSDVRPDFLVTSSYKWLLGPYGVG
                     YMYVGEEYREGKPIEHNWINRRGSQDFSGLVSYQDAFQPGARRYDVGERSNFALLPMA
                     AEALRQILDWEVENVSESIGTLTDLIEEKAGELGIATIPKERRARHMIGLMLGPDAPD
                     DLAARLTAHNVYVSVRGESVRVSPHLYNTEEDVGRLFEVLKRVVG"
     gene            complement(3601..5010)
                     /locus_tag="VN178_11575"
     CDS             complement(3601..5010)
                     /locus_tag="VN178_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS81652.1"
                     /translation="MVVVGGGIGGLAAAVLIGRAGHEVTLLEANPWVGGKSRRIELEG
                     RRMDTGPSLLTFPGVWEEFLRRWDATDGKPGEAAEIADLRLKRLPEVGRYYYRGEVTS
                     LPVEEGHPWRAAWERFAGIHGGLGPEVTRLLTADPSEGRTLPALGRLLRIYGAKLTTR
                     SYLDSLRWLPEGLREIIAIHTLNAGVAPTRTPALYASMPAIMAADGVLVPEGGVYEIV
                     LSLERLARRAGVEVRTGEAVLEIGRGTVVTAEREYTADRVVSDIDPGRLEGLLDPERG
                     RVKERLTCSGVAIYAPLREELPGGIGMHSVVLPSDPFELYASLEAGREPRETMAFLNY
                     YRGGEIYPNEGGTLALLLTAPANGLEYSLEDPFVRREIERVSAAAGLQEPVTRHFGEY
                     EVLDPGYFGAWGSAGGALYGAVRPLWRSGPLHRPPYKDRGRPWLWRVGASVHPGGGIP
                     AVLGGAMISTGRMLRSLVK"
     gene            complement(5016..5858)
                     /locus_tag="VN178_11580"
     CDS             complement(5016..5858)
                     /locus_tag="VN178_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWS81653.1"
                     /translation="MILRLLHISRPVLWINTVGPATVGMWLAGDLFAWGALPILLWLT
                     LPFNLLIYGVNDVFDQETDAKNPRKGSLEGARIDHAEVRPLVLGVILTNAPFLIYSLL
                     FAPFSATAWMLLYTLLFLGYSVPPARFKARPYLDSLSNAAYAFPLVFVPLALGEDPVW
                     PAALGLMAWSAAKHTFDAVQDVDEDRRAGITTTAVRLDPGGVVFWSGALWALATVCFA
                     LVSVPVALVNAVIAGALLYVLGQNPTPETGHRLYKYSIAFPYVAGTFAGVQLVAALSL
                     GAYP"
     gene            5914..6657
                     /locus_tag="VN178_11585"
     CDS             5914..6657
                     /locus_tag="VN178_11585"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HWS81654.1"
                     /translation="MTQLRRTTPRSLILACALSLVAAFFAVRYPDIQGASVGSYVSTL
                     LIALPSAVALFHYLGPRRATLSLLALSILAYAIEAIGVATGFPYGTFYYGDALGPRLA
                     GLVPYLLPLSYTPLVVGAVAAAWGTRPRLLRVLYATLLLVWMDAVLDPGAASLGFWVW
                     PEDGAYYGVPLSNYAGWLLSGALATTLLLTTGRWSETPRPALLDSAIIATSFWTGVSV
                     LSGMVAPALLGATLFAYLLGRRSYLRVAK"
     gene            6816..7037
                     /locus_tag="VN178_11590"
     CDS             6816..7037
                     /locus_tag="VN178_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81655.1"
                     /translation="MIRERATGGAGVGREDSAYRSGWEDGRFGQAQTFATNSSLAAWT
                     EYPERLSYYRGHRDGRRIREMLRSEGRAV"
     gene            complement(7293..7688)
                     /locus_tag="VN178_11595"
     CDS             complement(7293..7688)
                     /locus_tag="VN178_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWS81656.1"
                     /translation="MFSWFSWRMEELRETHEGGFTLIELMAVLVIVGILAAIAIPNYI
                     GQQDKARDAAAMAQLRMAATSQQLHYVDQNSYAGDATDLEAYGFRQGEQTVTVLAGDV
                     STYCMQAPGGGGVTFRITPAMGRPVPGAC"
     gene            complement(7897..>8280)
                     /locus_tag="VN178_11600"
     CDS             complement(7897..>8280)
                     /locus_tag="VN178_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWS81657.1"
                     /translation="TTCWVSQVSFAPQQVVVAVRKGQHTHDLIEQGGVFSLNFLDTEQ
                     EDLARKFTQPLQPEDGALAGSPYSTGETGAPLFEEAFAYLECQVVGKMEAGDHTVYLG
                     EVAAAHLARPADILTDLDTPMEYGG"
BASE COUNT         1532 a         2722 c         2551 g         1475 t
ORIGIN      
        1 ccgtacccgc aacaaggccg aggagctggc cagagcaggg tccgcgagag tcgcagacag
       61 cccgagggag gtcgccaggc agagcgccat aacgatcacc atgctccctg gtccgccaga
      121 ggtcgaggag gtcgtcgcgg gagaacgagg gctcctgaag ggtgccaggg aaggctcgct
      181 catcgtggac atgagtactt catcgccgat cctggcccag gagctggccc ggatcgcccg
      241 caagcgggag gtgggtatgc tcgacgcccc ggtcagcggg gccgacgtgg gggccgtgga
      301 aggaacactc tccatcatgg tcgggggaga ggaggaacat ttcgagcgcg cgaagtccct
      361 cttcgacgtg atgggggaga aggtagtcca cgtcgggcca ccggggaccg gccaggtcgt
      421 aaaagcctgc aaccagatcg tggtggccct caccatcgag gcggtctccg aggcgctggt
      481 gttaggatcg aaggccgggg tggatccggg gaagatcctg gacgtactct ccgggggact
      541 cgcggccaac aaggtcatgg aggtcaagcg cgagaagcta ctctcgcacg acttcgagcc
      601 gggcggcaaa gtcgagttcc atcgtaagga tctcgtcata gccctggaga caggcaggga
      661 gtatgagatt gccctgcccg taacgtccat ggtagaccag atgttcggct cccttatggc
      721 tgcgggacga ggcggctggg atcattcatc attggttacg ctcatcgagg attgggccgg
      781 ctaccgaccg gatcggcacg agcaatccta atagcgaaga ccttaacggc gacctgcatg
      841 gacgattacc acgcgcaacc cggcccggtt tattaccatt ttctcgggag gtatggatag
      901 tagataatgc taccttcggg ctcttgtcag tacgagggag aagcgatggc cggagaacaa
      961 agtagtcggg aaccaaccga gttgaaaaag gatctgggtg gtggattgat cctgcgccgg
     1021 gcaaggaggg aggacgccga ggcggtcgcg gcgttcaacg cgcggatcca ccactcctcg
     1081 ggagatttcg accagaggga accccatcgc gggatagccg ccggcacgcg cgacctgatg
     1141 tcgggggatc atcctacctg cgatgcctcg gacttcacgg tggtcgagga caccaagacg
     1201 ggcttcgtag tctcctcgac gtgcctgatc gggcaacgct tctcctacga aggggtggag
     1261 ttcgacgccg gactacccga gctggtcggc agccacccgg actaccgccg tcgcggcctg
     1321 gtgagggagc agttcgaggt actacaccgc tggagcgaga agcgcgggca cctgatgcag
     1381 gcgataggcg gcatacccta ctactaccgc cggttcggct acgagatggc cgtgtacatg
     1441 ggcgggggac gccggatcta cgtccaggac gtaccagaca agccgtcgag ctccgggggg
     1501 gccgagagct ctccccgctc ctaccggctc cggcccgccg cagcggcgga cgctgggttc
     1561 ctctcgaatt tggaccgccg gggaagagga cgcggcctgc tcaccgcctc gcgcgatgag
     1621 ggtctttggc gctacgaggt ggccgggcgc gacccggaga gcgacgagtc gctggagatg
     1681 aagatcgtgg aggatgccac cggcgaatcc gtgggatacg tctgccacgc gcccgacctc
     1741 cgaaatgggg ccctgcaggt gtacggctac gagctcgcgg acggcatctc ctggctcgag
     1801 gccaccccct tcgtgctaag cgagttggca gagttcggac gcaagcgtgc ctcgggcgag
     1861 gaagagctcg cgtcgttgac gttcgttctc ggcgagcagc atcccctcca cgaggccatc
     1921 ccggagccgc cgctctacag gctcgaccgc cagggcaact actcctacta cgtgcgggtg
     1981 cccgacctcc ccgggttcct tcgccacgta gcgccggtgc tcgaacggag gctcgcggct
     2041 tcggtggccg cggggcacac ctgcgagcta aaggtaagct tctacggcgg tggcattcgc
     2101 ctcgagctgg atcgcgggcg gctgagtaga gtggaggact ggagccccac ggtcgaagag
     2161 cgcggcgacg cggcctttcc ggacctgacc ttcctgcaac tcctcttcgg ttaccgctcc
     2221 ctcgacgaac tcgaccgcgc cttcgccgac tgtttgccag gcgagggaga cgctcgagcc
     2281 ttgctcaaag ccctgttccc caggcgtccc tccgatctct ggcccgtctt ctgaggcgtg
     2341 tagtatgcct cctgccccgc gcagcgccaa agaatagtcg gggcgtgttc tcgagaactc
     2401 ctgaaacctg aacccgtgct tctcgaaccg ctctaaccaa caacccgctt taggacctcg
     2461 aagagccgac cgacgtcctc ttcggtattg tagaggtgag gcgagactcg cacactctcg
     2521 ccgcgcacgc tgacgtagac gttgtgtgcc gtgagcctgg cggcgaggtc gtcgggggcg
     2581 tcagggccga gcatcagccc gatcatgtgc cgcgcccgcc tctctttggg gatcgttgcg
     2641 ataccgagct ctccggcctt ctcttctatg aggtccgtca acgtgccgat ggactcagag
     2701 acgttctcga cctcccagtc gaggatctgg cgcagcgcct cggccgccat agggagcagg
     2761 gcgaagttgc tccgttcccc cacatcgtag cgcctggcgc caggctggaa ggcgtcctgg
     2821 taagaaacca gccccgagaa gtcctggctc ccgcggcggt tgatccagtt gtgctctatt
     2881 ggcttcccct cccgatactc ctccccgacg tacatatacc cgacaccata gggtccgagc
     2941 agccacttgt acgatgaggt gacgaggaag tcgggccgaa catccgagac gtcgaaggga
     3001 tacgccccaa gcgactgtat cgcgtcgacg accagcgccg cccctacttc gcggaccctt
     3061 tcgccgacgc ttgcgaggtc gacgagcgat ccgtcggtcc agtggcagtt cgggacggca
     3121 acaacggcgg tgtcggcgtc gatctccccg accagggccc gaccccagtc gagatcttct
     3181 ggacggggta aggtgacgag cttcgccccg ctccgctcgg ccagctcgcg ccaagcgtag
     3241 acgttggagg gaaactggtc ctcgaggatg acgatcttct ctccttctcg caccggtacg
     3301 ttcgccgccg caacggagat cccatagctg acggagggga ttaccgcgac gccgtcggcg
     3361 tcaccgccga cgagccgcgc gaacaacgcc cgcgcctctt ctgcttcctc gaagaagtcg
     3421 tcaggcgtga tctcccaggg ccgcgacttg cgcgacactg ccttctcccc ggcctcccgc
     3481 gccggacgta gctgcggcgc catatacgcg cagttcagat aaacgatgtc ttctggtatc
     3541 tcgaacaggt cgcgctggct gctgaggttc atccctaacg tccctcctgt actttattcc
     3601 tcacttaacc aaagatcgga gcattctgcc cgtcgagatc atggcccccc cgagaacggc
     3661 cggtatgccg cccccgggat gcacggaagc cccgacccgc cacagccacg gacgtccacg
     3721 atctttgtaa ggtggcctgt gcaaaggtcc gctcctccac aacggacgca cggccccgta
     3781 tagcgccccg cccgcactcc cccaagcgcc gaagtacccc gggtcgagga cctcgtactc
     3841 cccgaaatgt ctcgtcaccg gttcttgcag tcctgccgca gccgagacgc gttcgatctc
     3901 ccgcctgacg aacgggtcct ccagactgta ttccaatccg ttcgcaggcg ccgtgagcaa
     3961 taaggccagc gtccctcctt cgttggggta gatctcaccc ccacgataat aattgagaaa
     4021 cgccatcgtc tcccgcggtt ctctgccagc ttcgagactc gcgtacagct caaagggatc
     4081 ggaaggcagc acgacgctgt gcatcccgat tccgcctggt agctcttcgc ggagcggcgc
     4141 gtaaatggcc accccggagc aagtcagcct ctccttcacc cgccctcttt cgggatctag
     4201 caggccctcc agcctgcccg ggtcgatgtc gctaaccacc ctgtctgctg tgtactctcg
     4261 ctccgccgtc acgaccgtgc cgcgcccgat ctcgagcaca gcctcgcccg ttctgacctc
     4321 gacacctgcg cgtcgggcga gtctctcgag agaaagcacg atctcgtaga cgccgccctc
     4381 gggcacgagt acaccatcgg cggccatgat cgcaggcatg ctcgcataca gcgcgggcgt
     4441 ccgcgtcggc gccaccccgg cgttgagcgt gtggatggcg atgatctccc tcaacccctc
     4501 gggcaaccat cgaaggctgt ccaggtagga gcgggtcgtc agcttcgccc cgtatatcct
     4561 tagaagacgt cccagcgcgg gtagcgtcct accctccgaa gggtcagccg tcagtagccg
     4621 tgtgacctcg ggcccgagcc cgccgtgtat gccggcgaac cgctcccacg ccgcacgcca
     4681 gggatggcct tcctccacag gcagcgacgt tacctcgccg cggtagtaat accgtcccac
     4741 ctcgggcagc cgtttcagcc tgagatccgc gatctcggca gcctcgccgg gcttaccgtc
     4801 agtcgcgtcc cagcgccgca gaaactcctc ccacacaccg gggaaggtga ggagcgaagg
     4861 tcccgtatcc atccgccggc cttcgagctc gatgcgccgg ctcttaccac caacccacgg
     4921 attggcctcc aacagcgtaa cctcatgccc ggcgcgtccg atgaggaccg ccgcggccag
     4981 ccccccgatg ccgccgccga cgacgaccac gcggctcaag gataagcccc cagagacagc
     5041 gcagcgacga gctgcacgcc ggcgaaggtc ccggccacgt aagggaaggc tatagagtac
     5101 ttgtagagcc ggtgtcccgt ctccggcgtg ggattctggc ccaggacgta caacagcgca
     5161 cccgctatca cggcgttcac cagcgcgacg ggcacgctaa cgagggcgaa acaaacggtg
     5221 gcgagcgccc agagagcacc gctccagaag acgacgccgc cagggtcgag cctgaccgcg
     5281 gtcgtcgtga tgccggccct gcggtcctca tccacatcct gcacagcatc gaaggtatgc
     5341 ttggccgcgc tccacgccat gagtccgagc gcggctggcc agacgggatc ttcgccgagt
     5401 gcgagcggaa cgaaaacgag cgggaaggcg taggccgcgt tgctcagcga gtcgaggtat
     5461 gggcgggcct tgaagcgtgc gggtgggacg gagtaaccga ggaagagtag cgtgtagagt
     5521 agcatccagg ctgtggcgga aaagggcgcg aacaggagag agtagatcag aaacggcgca
     5581 ttcgtaagga tgacgccgag cacgaggggg cggacctcgg cgtggtctat cctggctcct
     5641 tccaggctgc ccttgcgggg atttttggcg tcggtctcct ggtcgaatac gtcgttgacg
     5701 ccgtagatga gaagattaaa agggagcgtc agccacaaca gaatcggcag cgctccccaa
     5761 gcaaagagat cccctgccag ccacatcccg accgtggccg gacctacggt gttgatccag
     5821 agcacgggcc gcgagatgtg gaggagacgc aggatcacag cccctaattt ataccagcgt
     5881 cccccttgca gaaccgttaa cattgcaagg gtgttgaccc agctccgccg caccacaccg
     5941 cgatccctga tcctcgcctg cgccctctcc ctggtggccg cattcttcgc cgtacgctac
     6001 ccagacatac agggagcaag cgtaggctcg tacgtctcga ccctccttat agccctgccc
     6061 tcggccgtcg cactcttcca ctacctgggc ccacgccggg ccacgctctc cctgctcgcc
     6121 ctctccatct tggcctacgc catcgaagcc ataggcgtcg caacaggctt cccctacggt
     6181 accttctact acggcgacgc cctcggaccc aggctcgccg gactcgtccc ttacctcctg
     6241 cccctctcct acacgccact cgtcgtcggg gccgtggcag cagcgtgggg gacccgcccg
     6301 cgtctcctcc gcgttctcta cgctaccctt ctgctcgtct ggatggatgc cgtcctcgac
     6361 cccggtgcag cctccctggg cttctgggtg tggcccgagg acggcgccta ctacggcgtg
     6421 ccactcagta actacgccgg ttggctgctc tcaggagccc tcgccaccac cctgctcctc
     6481 actaccggaa ggtggtccga aacaccaaga cccgccctgc tcgatagcgc catcatagcc
     6541 acctccttct ggacaggcgt gagtgtcctt tccggtatgg tggccccagc cttgctcggt
     6601 gcaacgctct tcgcatacct cctcggacgc cgctcgtatc ttcgtgtggc gaaatagtaa
     6661 caatagacac aatatttaca gcgcgtacgg tctaacggcg cccaatatgg aaattttgta
     6721 atttttgttc gattttatta agttcaggcc ttacatttat gaatccattg tgtttatatg
     6781 atgcttgtaa gaatcacgcg cccgggaggt ggggaatgat ccgagagaga gccactggag
     6841 gggctggggt tggccgcgag gattcggcct acaggagcgg ctgggaggat gggcgcttcg
     6901 ggcaggcgca gacgttcgcg accaacagca gcctcgcggc gtggaccgag tatccggaaa
     6961 ggctttccta ctaccgcggc caccgcgacg gccggcgtat tagggagatg ttgaggagcg
     7021 aaggccgggc tgtttgatcc gccgtaacag gctgaaggat cctacctgcg gatctagctg
     7081 atcactcctc ccaattccgc aacctcatag ggcaagctgc gtatctatag gggagggccg
     7141 acggctcccc ctccaagttc gccgtcgacc ctcatcgtat gttagcgaaa gtcgcgggcg
     7201 agttccattg cccctcaggt gtccctttct cataggtgac aggaccgcat cggctgagca
     7261 gaggactctc agctcgaacc accggagcac tcctagcatg caccaggcac aggtctgccc
     7321 atagcagggg tgatcctgaa cgtgactcca ccgccgccgg gggcttgcat acagtaggta
     7381 cttacgtcgc ccgccagtac cgttaccgtt tgctctccct gcctgaaacc gtaggcctcg
     7441 aggtctgtgg cgtcgccggc gtaggagttc tggtccacgt aatggagttg ctgcgacgtc
     7501 gccgccatac gcagttgcgc catggcggca gcgtccctcg ccttatcctg ctggccgatg
     7561 tagttcggga tggcgatggc agcgaggatc ccgacgataa cgaggaccgc catcaactca
     7621 ataagtgtga aacccccctc atgcgtctca cgaagctcct ccatccgcca cgagaaccag
     7681 gaaaacacct ttctcccgtt ccttctgcac acctctgcca ccgccgggtc tccgcacgct
     7741 ccacgtactt actcggcgat acgcccacgg ttctttaacg cgccggaatc agaacctggg
     7801 gagtccctga aaggctgacc agccgaaatg ctgacgagcg agggctaaaa gcctgagcgt
     7861 gctgacatgc tgacaagccg cgaagcggcg tgctgattac cctccgtatt ccatgggggt
     7921 atcgaggtcg gtgagaatgt ctgcgggacg cgccaggtga gcggcggcga cctccccgag
     7981 gtagaccgta tggtcgccgg cctccatctt gccgaccacc tggcactcca agtaggcgaa
     8041 cgcctcctcg aagagcgggg cacccgtctc tcccgtagag tatggcgagc cggcgagggc
     8101 accgtcttcg ggttggaggg gctgagtgaa cttgcgggcc aggtcttcct gctccgtatc
     8161 gaggaagttc agggagaaga cgccgccctg ttcgatcagg tcgtgcgtgt gctggccctt
     8221 gcggacggcg acgacgacct gctgcggcgc gaaggagacc tgcgagaccc agcaggtggt
//
LOCUS       DATMVF010000223        12287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_215932, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000223 DATMVF010000000
VERSION     DATMVF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12287
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215932"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VN178_11605"
     CDS             complement(<1..761)
                     /locus_tag="VN178_11605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS81658.1"
                     /translation="MSAAVNKSAEVRRGPSRLKAWWSNPWGKPRFLVLCTIAYILWSL
                     VPVAIAVQFGFNAGRSRSAWQGFSLRWYFQDPNLSVLHDPSLRAALVQSLQLAALTML
                     IATPLGVALAIGLARWTGWGARPANFLMLFPLVTPEIVMGVALFLVFVHLFGFVRLGT
                     MAQVLGHVTFTVSYVVIVVRGRLFSVGREYEEAAMDLGASRWQALRTVLLPMLAPAIW
                     ASLMVAFAISIDDFVISAFLMGDASSTTIPVKLYSA"
     gene            complement(758..1681)
                     /locus_tag="VN178_11610"
     CDS             complement(758..1681)
                     /locus_tag="VN178_11610"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS81659.1"
                     /translation="MSGPDEGGRRAVGEKRWLWPSLAAPGVIWLVLLFLVPLYAVLAV
                     AMGAPDPIFGGALPAWNPLAWDPSEFGTILGEIFGGQLGFVFLRTLIYVAVATFLSLL
                     IGYPVAYYVSRHAGRWRVPLLLLIIAPFWINYLMRMLAWVNLLSTDGYVNQVLMFVGI
                     LDAPREWLVGKHSTVILGLVYGYVPFLILPLFAALDRIDPRVIEAARDLGASPARAFF
                     AVTLPLSSQGILAGCVIVMLPMFGDYYTATLLSGSPKTNMVGKLIEQDILSGAGSGRG
                     AALTVVLMMFVGILMTYYLYSVARASREARS"
     gene            complement(1686..2951)
                     /locus_tag="VN178_11615"
     CDS             complement(1686..2951)
                     /locus_tag="VN178_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWS81660.1"
                     /translation="MAFHPRERSPWRRPMSRREMLGLVAGTAVAGGLLAGCGSSSGSG
                     SASQLAIGTRENPVEQPLFDDIPRIESGLEPEEGPLRVYNWADYINPAVLKSFTKEFG
                     NEVEVTTFYNSQEAAQKLRTGKISFDIFFPTQDDLPKYVAGKLLQPLNHDYIPNLKKN
                     VWPTLADPFYDRKSRYTAPYVTYSTGIGWRTDMVSTDVASMENPWDVLWDSEYRGVTG
                     LYDEYRDALSVGMYRSGITNVNGAASSDLEKARDNLLELPDLMEIRYTIDGAYSRLPE
                     GNLGLVQAWSGDTLLAIQYAPNAEDVSVLRYAWPPKTTQNKVGGMISNDCMAVLANAE
                     RPVLAHAFINYVLDAKVALQNMSWLGYQPPQKSLDPTSMVDRGLVPETLATAVVAQED
                     FELGQITTQLTPQEEGRWLESWSRVQQGG"
     gene            complement(2988..3653)
                     /locus_tag="VN178_11620"
     CDS             complement(2988..3653)
                     /locus_tag="VN178_11620"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS81661.1"
                     /translation="MGARPKLTQKRRTQMLEAAMEVIAERGLGETRIADVADRAGLSP
                     ALVVYYFGSKETLLTEALAYAEDLFYVEAFRELTDIEGASERLVRLIELACPPVVSRE
                     SDEYWKLWVELWSRALRNEEAARKREALERRWRNTIAEVVREGQRSGEFGPCDSERFA
                     TYLSALMDGLALQVLFGDPAVSGELANEMCLGAAARELGFDALGLRGGQGSVGQKELA
                     AEG"
     gene            3839..5476
                     /locus_tag="VN178_11625"
     CDS             3839..5476
                     /locus_tag="VN178_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS81662.1"
                     /translation="MMRGGYEAIVIGGGHNGLAAGAYLARDGLRTVVLEARHKVGGAA
                     TTDAPWQEAPEFNVTTYSYVMSLMPRRIIDELRLEHHGYKVFPMGPSYYAFPDGRSLT
                     MTGVDAGKDYEALARFSRRDAEAYGEWRKWLGELAAIMEPLLLTPPPKIGSRHPLDLL
                     DQLKLAWRMRGLDVPRVGELTRLMTMSAWDLLDDWFESPQVKGAMAVDGIIGTWAGPA
                     TPGTAYVLMHHEIGDAGLGLSSWGYPRGGMGAVSDALRSSAEAFGAEVRTNAPVERIL
                     QKDGRVTGVVLESGEELEADIVVAATHPRITFLEQLGSEELPDDFVGNIERWNSRSGV
                     VKINLALSELPDFTADPGRGEHLGGAVELAHSIDYIEEAFQDARRGRAARRPFSDGVI
                     PTVFDRTLCPEGYHIMSLFTQWVPHEWSEEPHREELEAYADRVIDGYSELAPNLRGAI
                     LHRQVLGPYDMEQEIGLIGGNIFHGELSAEQLFHMRPAPGYADYRTPIRGLYQASSAT
                     HAGGGVCAIPAYNCVREIRRDRRRERLKQRLTVRNRG"
     gene            5481..7589
                     /locus_tag="VN178_11630"
     CDS             5481..7589
                     /locus_tag="VN178_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWS81663.1"
                     /translation="MAVDEALRAMLEARSVAMVGASPRFDTPGNYMVKQLIIGGFSGE
                     AAAVNPKYDEVEGIACFPGLDELPFVPDLVLLAVGNRRLERQMEEAARCGARGVVVFA
                     SGLEDPPDDPPLTERLKSIAADAGMAVCGANCMGFADVERGLRALAFEERADLEPGNV
                     AWISHSGSAFSALLHAERGIRFNLAVSTGQELTTTMADYMLYALERESTRVISLFLET
                     VRDSQRFRLALETAAGRDVPVVALKVGRTPTSRRLVTAHSGALAGDDAAYEALFDAYG
                     VARVGSLNEMADLLELLGSERRAAPGGLAAILDSGGERAHLVDAAADLEVPLARPSEE
                     TLRDVAARLEPGLPAVNPLDAWGTDNDPYNIFVDCSRYLVDDPDTGAFAYVVDLHSDR
                     AERGHAWAAERVWNSTQKPFAVVCGVTSAIQESAAARLRSSGIPVLEDVTSGLRAFLH
                     LFERRDARRPSSQEPPDPPPRETVRRWRETLIAGEDLGEARTLEMLTDYGVPVARTAA
                     ATDIEGVLAAARRLGYPVALKTAAASHKTELGGVTLGIRDEDGLTGAYREMSSHLGPS
                     VTVQEMVDPGVEMALGMVHDPQFGPLVMVAAGGVLIETLGDRRLALPPLDEARASKLI
                     DRLDVRPLLDGVRGAPPADVNSLARTLSRLSLLAGDLGDLIGALDVNPVIVGPRGCVA
                     VDALVEQSANGFQQEKAESR"
     gene            complement(7586..8752)
                     /locus_tag="VN178_11635"
     CDS             complement(7586..8752)
                     /locus_tag="VN178_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWS81664.1"
                     /translation="MTPIDRELLASSLVSEGQMSLPAEAYTDDAVLDWEMEHFFDESW
                     VCVGRSEDLRGVGDRCAVALGHESALLARDEGGNLRAFYNVCRHRGHVLAEVGESSSG
                     RFIRCPYHAWAYGLDGSLQGAPGFGQLPGFDKSDYSLVPVRVAEWEGWLFANASGEAP
                     PFEEHVGSLGEMIGNHRSGELVTAARHSYEVAANWKILVENYHECYHCSSIHPELCKV
                     SPPKSGEDHEARGIWFGGSMELEDHAETMSLTGESFAPKLPHLTALQEREVYYYQLFP
                     NLLISLHPDYVMTHRLRPLAPGRTAVECEWLFSKKAVESEGFDPSYASEFWDVTNGQD
                     WGACEGVQSGASSRGYRQGPLSPDESTSGKFVATVARGYLEGGISHVPDQPAVS"
     gene            9109..10287
                     /locus_tag="VN178_11640"
     CDS             9109..10287
                     /locus_tag="VN178_11640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS81665.1"
                     /translation="MRIYDVVVVGGGNLGLWTAHRLARRGFGRIAVLERGWAGGGATS
                     RSAGVVRQQGGSETAAKLGKLSRELYLRLGEELGLDSGFRETGYFIVAETEEEKEGFL
                     RLVEVRLAAGVENEWVEPEEGRRRFPDLNWNRFVGATYTADDGYVHPPVAARNATCAV
                     LGDDTVDLFEMCEVTGVGQKGDHYSVESACGTFEAERVVDAGGPRGVRDVGGMVGVEV
                     PVMAVRHQIVSFPYLPEGFEHPFPLVFNVGKGYYWRPEEQGVLLGMSNPVDEADETGR
                     YQIDFDWDYYARMRPDWESAHPALRGLPVARAWAASIDYTPDHLPIIDEPMEGFYVLA
                     AGGHGMMWGPALGEKMAGLISEGRVEDLPEEDIELARFSRERDPNRIEDTIALPFPKE
                     "
     gene            10302..11480
                     /locus_tag="VN178_11645"
     CDS             10302..11480
                     /locus_tag="VN178_11645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS81666.1"
                     /translation="MTTAPQMTAEIPQEVKALKARIAEFVREELHPFEAEIAERGEID
                     EEKLRALRNKARRAGFSNLNMPAEYGGMDLSMVSLVALEEESGKVTNGLGYMVADRGP
                     RELLELASAEQVERYVMPVLRGETREAWAITEPEAGSDIKDIKTTAKRDGGGWVLNGE
                     KWFVTGGERAGFFIVLAWAGEEQASFLVERETPGLEFVRTPVFMHDPYASKHVELRLK
                     DCRVPEGARLGSGGDEGTKRWFSLERLMIAARCCGAAERLIDLARSWSLERQASGKKI
                     SEYQAIQFMLADSVTELNAARLMVNHVARMWEVEAPSILHGKTAMAKLFASEMAGRVA
                     DRAVQVFGGRGYMTENPAERYYRELRVDRIWEGTSEIQRSIIARGLLKRGREAYTMDW
                     "
     gene            complement(11536..>12287)
                     /locus_tag="VN178_11650"
     CDS             complement(11536..>12287)
                     /locus_tag="VN178_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWS81667.1"
                     /translation="PPMMFTDEEALALALGLLSARRLGLSGAAPAVEGARAKLERVMP
                     EELRERVRTFEEVVVPAASAPARLPAGQVVVTLSEAVRERKRVWMRYRAGDAGETRRV
                     VDPYAVVQGDAFWYTFGYCHLREGRRLFRLDRVLSVEMLDEAFVLPPGFDTPQGVLEA
                     LADMREDRWSVEVLLETTPGEARGQLPKMGVSLQEAPEGVLMRSSTSDLMWMARVLAG
                     LSFPFVVREPPELRESLRRLAAEIEALAERT"
BASE COUNT         2216 a         3947 c         4104 g         2020 t
ORIGIN      
        1 gcgctgtaga gcttgaccgg tatggtggtg gacgaggcgt ctcccataag gaaggcgctg
       61 atcacgaagt cgtctatgga gatggcgaag gcgaccatga ggctcgccca gatcgccggc
      121 gccagcatgg gcagcagcac cgtcctgagc gcctgccacc tggaggcgcc gagatccatg
      181 gccgcctcct cgtactctct tccaacggag aacagccgtc ctcgtacgac aatgacgacg
      241 taggagacgg tgaacgtcac gtgcccgagg acctgggcca tcgtaccaag ccgcacgaag
      301 ccgaagaggt gcacgaagac cagaaacaac gcgacgccca tcactatctc gggcgtgacg
      361 agtgggaaga gcatcaggaa gttggcgggc cttgcgcccc accctgtcca gcgcgcgagc
      421 ccgatggcca atgcgacccc taagggtgtg gctatcagca tcgtcagcgc tgcgagttgg
      481 aggctctgga cgagcgcggc ccgcagcgac ggatcgtgca gtacggagag gttcgggtcc
      541 tggaagtacc agcggagcga gaaaccttgc caggcgctgc gcgagcggcc cgcgttgaaa
      601 ccgaactgca cggctatcgc cacggggacc agcgaccaga ggatgtaggc tatcgtgcag
      661 agcaccagga aacgcggttt gccccagggg ttggaccacc acgccttgag acgcgatggc
      721 ccccggcgca cctccgcact cttgttcacc gcggcgctca acttctggct tccctcgacg
      781 cccgcgccac gctgtagagg tagtaggtca tcaggatgcc gacgaacatc atcagcacga
      841 cggtaagggc cgcacccctg ccgctgcctg cgccgctcag gatgtcctgc tcgatgagct
      901 ttccgaccat gttcgtcttg ggagaccccg agagaagcgt ggccgtatag tagtcgccga
      961 acatcggaag catgactatg acgcaccccg caaggatgcc ctgcgaggaa aggggcaagg
     1021 ttacggcgaa gaaggcccgc gccgggctcg ccccgaggtc gcgcgccgcc tcgatcaccc
     1081 gcgggtcgat gcggtcgagc gcggcgaaga ggggcaggat caggaacggc acgtaaccgt
     1141 agacgagacc caggataacg gtggagtgct tgccgaccag ccactccctc ggcgcatcga
     1201 ggatgcccac gaacataagg acctggttga cgtagccgtc cgtggagagc aggttcaccc
     1261 acgcgagcat gcgcatgagg tagttgatcc aaaacggcgc gatgatgagc aacaggagcg
     1321 gcacgcgcca gcgtccggcg tgccgggaga cgtagtaggc gaccgggtac ccgatcagga
     1381 ggctcagaaa cgtggcgacc gcgacgtaga taagcgtacg caggaagacg aagccaagct
     1441 ggccgccgaa gatctcgcca agtatcgtcc cgaactcgct cgggtcccac gccaacgggt
     1501 tccatgctgg cagcgcgcca ccgaagatgg ggtcgggcgc tcccatggcc acggcgagaa
     1561 ccgcgtagag cgggacgagg aagagcagga ccagccagat gacgccagga gccgccagcg
     1621 agggccacag ccagcgcttc tcgccgacag cgcgacgccc tccttcgtcg ggcccgctca
     1681 cgctgctagc ctccctgctg gaccctggac caggattcga gccagcgtcc ttcttcctgc
     1741 ggggtcagct gcgtggtgat ctggcccagc tcgaagtctt cctgcgcgac gaccgccgtt
     1801 gccagggtct cggggaccag ccccctgtcc accatgcttg tcggatcgag ggacttctgg
     1861 ggcggctggt aaccgagcca gctcatgttc tggagcgcga ccttcgcatc cagtacgtaa
     1921 ttgatgaacg cgtgggcgag cacgggacgc tccgcgttcg cgagcaccgc catgcaatcg
     1981 ttggagatca taccccccac cttgttctgc gtcgtcttgg gcggccacgc gtagcgcaac
     2041 accgagacgt cctcggcgtt aggcgcgtac tggatcgcca ggagcgtgtc ccccgaccag
     2101 gcctggacca gaccgagatt gccctcgggc agcctggagt aggccccgtc gatggtgtag
     2161 cggatctcca tgaggtcagg aagctccagc aggttgtccc tggccttttc caggtccgag
     2221 gacgcggcac cgttgacgtt cgtgatgccg ctcctgtaca tgccaacgct cagcgcgtcc
     2281 ctgtactcgt cgtagaggcc cgtgacgccc ctgtactcgg agtcccatag tacgtcccac
     2341 gggttctcca tgctcgccac gtccgtcgaa accatgtccg tccgccatcc gatacccgtg
     2401 ctgtaggtca cgtagggggc ggtgtaacga gattttctgt cgtagaaggg gtcggcgagc
     2461 gtcggccaga cgttcttctt taggttgggg atgtagtcgt ggttgagcgg ctgcagtagc
     2521 ttgcccgcga cgtacttcgg caggtcgtcc tgcgtgggga agaagatgtc gaagctgatc
     2581 ttgccggtcc tcagcttctg cgccgcttcc tgcgagttgt agaaggtggt tacctccacc
     2641 tcgttcccga actccttggt gaacgatttg aggaccgcag ggtttatgta gtccgcccag
     2701 ttatagacgc gcagaggacc ctcctccggc tcgagccccg actctatgcg cgggatgtcg
     2761 tcgaatagcg gctgctctac cgggttctcc ctggtcccga tcgcgagttg cgaagccgaa
     2821 ccgctaccgg aagagctgcc acaacccgca agcaatccgc ccgccacggc cgtaccggca
     2881 accaagccga gcatctcgcg gcgcgacatc ggccgccgcc agggcgatct ctccctcggg
     2941 tggaacgcca tctctatcga cctcctctgg gaccccaggc cccgtcctta cccctcggca
     3001 gcgagctcct tctgtcccac gctcccctgc ccacccctca gcccgagcgc gtcgaagccc
     3061 aattccctgg ccgccgcgcc aagacacatc tcgttggcca gctccccact caccgcgggg
     3121 tcgccgaaaa gtacctgcag cgcgaggccg tccatcagcg cggagaggta agtcgcgaag
     3181 cgctcggagt cgcaaggtcc gaactctccg gagcgctggc cctcccttac tacctcggcg
     3241 atggtgttgc gccaccggcg ttcgagcgcc tcgcgcttgc gggccgcctc ctcattgcgt
     3301 agagcccgcg accacaactc gacccacagc ttccagtact cgtcgctctc gcgactcacc
     3361 acaggcggac acgccagctc gatcaaacgc accagacgct ccgaagcgcc ctcgatgtcc
     3421 gtcaactccc ggaaagcctc tacgtagaaa aggtcctccg cgtaagcgag cgcctcggtc
     3481 agaagcgtct ccttggaccc aaagtagtac accaccaacg ccgggctcaa accggccctg
     3541 tccgccacat cggcgattcg cgtctcgcca agaccccgct ccgcgatcac ctccatggcc
     3601 gcctccagca tctgcgtgcg acgcttctgc gtcaacttgg gcctcgcgcc catcctcctc
     3661 ctacggtcta catccgaact tgtgaatggc tatttaatgt accaccatag cataagcgtc
     3721 ccaatcagtc cacaattacc cgtaatccgc gaccggtaag aaacgtcttg ccaccgcaaa
     3781 cgcaagatgt tacattgaat ggcgatttag aatcagtcgc aggcttgtga gaggagagat
     3841 gatgaggggc ggttacgagg ccatagtcat cggcgggggg cacaacgggc tggccgcggg
     3901 ggcctatctc gcccgggacg ggttgaggac ggtggtgctc gaggcccgtc acaaggtcgg
     3961 cggggcggcc acgaccgacg cgccctggca ggaggccccc gagttcaacg tcaccaccta
     4021 ctcgtacgtg atgagtttga tgccccggcg tatcatcgac gagttgcgat tggagcatca
     4081 cgggtacaag gtatttccga tgggaccttc ttactacgcg ttccccgacg ggcggtccct
     4141 gaccatgacc ggggtggacg ccgggaagga ctacgaggcg ctggcgcggt tctcgcgcag
     4201 ggacgcggag gcgtacgggg agtggcggaa gtggctcggg gagctggcgg cgatcatgga
     4261 gcctttgctg cttacgccgc cgccgaagat aggatccagg catccgctgg acctcctgga
     4321 tcagctgaag ctggcgtggc ggatgcgggg cctggacgtg ccgcgggtgg gcgaactcac
     4381 ccggctgatg acgatgagcg cctgggatct gctcgacgac tggttcgagt cgccgcaggt
     4441 aaagggtgcg atggcggtcg acggcattat cgggacgtgg gccggtcctg cgaccccagg
     4501 gacggcttac gtcttgatgc accacgagat tggggacgcc ggcctcggcc tcagctcctg
     4561 gggatacccc aggggaggta tgggagccgt ctccgacgcc ctcaggagtt cggcggaggc
     4621 gttcggcgcc gaagtaagga ccaacgcccc ggtcgagcgt atcctgcaga aggacgggcg
     4681 cgtaactggc gtggtgctcg aaagcggcga ggaactggag gccgatatcg tggtggcggc
     4741 gacgcacccc aggatcacgt tcctggagca actcggaagt gaagagttgc ccgacgactt
     4801 tgtgggcaac atagagcggt ggaactcccg cagcggggtg gtcaagatca acctcgccct
     4861 ctcggagcta cccgacttca ccgcagaccc gggtcggggg gagcacctcg gcggtgccgt
     4921 ggagctggcc cactccatcg actacatcga ggaggccttc caggatgcgc ggcgcgggag
     4981 ggcggcgcgc cggccgttct ccgacggcgt tatacccacg gtgttcgacc gcacgctctg
     5041 ccccgaggga taccacataa tgtcgctctt tacgcagtgg gtcccgcacg aatggtccga
     5101 ggagccccac agagaagaac tcgaggctta cgcggaccgt gtgatcgacg gctacagcga
     5161 gctggcgccg aatctcagag gggcgatcct gcaccgccag gtgctaggtc cttacgacat
     5221 ggagcaggag atcgggctca tcgggggcaa catcttccac ggcgagctct cagccgagca
     5281 gctcttccac atgcggcccg cccccggtta cgcggattac cgcaccccaa tccggggcct
     5341 ctaccaggcc tcgtcggcca cccacgccgg cggcggggtg tgcgccatcc cggcgtacaa
     5401 ctgcgtgcgg gagatccggc gcgaccggcg cagggagcgc ctgaagcaac gcctgaccgt
     5461 gcgcaaccgc ggatagcaag gtggcggtgg acgaggccct gcgcgccatg ctggaggcgc
     5521 gttcggtcgc gatggtcggc gcgagcccga ggttcgacac cccgggcaac tacatggtga
     5581 aacagctcat catcggcggc ttctccggcg aggcggcagc ggtaaacccc aagtacgatg
     5641 aggtggaggg aatcgcctgt tttcccgggc tcgacgagct gcccttcgtc cctgacctgg
     5701 tgctacttgc tgttggcaac cggcggctgg agaggcagat ggaggaggcg gcccgctgcg
     5761 gcgctcgggg cgtcgtcgtg ttcgcgagcg ggctcgagga cccgccggac gatcctccgc
     5821 tcaccgagcg tctcaagagc atcgccgccg acgctggtat ggccgtctgc ggcgccaact
     5881 gcatggggtt tgcggacgtc gagcgcgggt tgcgcgccct ggccttcgag gagcgcgcgg
     5941 atctcgaacc agggaacgta gcctggatct cccactccgg ctccgccttc tcggcgctgt
     6001 tgcacgcgga gagggggata cgcttcaacc tcgccgtctc gacgggccag gagctgacga
     6061 ccactatggc cgactacatg ctctacgccc tggagcgcga gtcgacgcgc gtgatctcct
     6121 tgttcctgga gacggtcaga gactcccaga gattcaggct tgcgctggag accgccgccg
     6181 ggcgcgacgt gcccgtggtc gcgctgaagg tcgggaggac gcccacctcc cgccggcttg
     6241 tcacggccca ctccggcgcg ttggccgggg acgacgccgc ctacgaagcc ctgttcgacg
     6301 cctacggagt ggcccgcgtg gggtcgctca acgagatggc cgacctcctg gagctcctcg
     6361 ggtcggagcg ccgcgccgcc ccgggaggcc tggctgccat cctcgactca ggcggagagc
     6421 gggcgcacct ggtcgacgcc gcagcagacc tcgaagtccc cctcgccagg ccctccgagg
     6481 agaccctaag ggacgtcgca gcgcgactgg agcctggcct gccggccgtc aaccccctgg
     6541 acgcttgggg tactgacaac gatccttaca acatcttcgt ggactgctcc aggtatctgg
     6601 tcgacgatcc cgacacgggg gcgttcgcct acgtggtaga cctgcacagc gatcgcgccg
     6661 agcggggcca cgcctgggcc gcagaaaggg tctggaactc gacgcagaag ccgttcgcgg
     6721 tggtctgcgg cgtcacgagc gcgatccagg agagcgccgc ggcgcgcctg cgctcctccg
     6781 gtatacctgt tctcgaagac gtaacctcgg gactgcgcgc ctttctgcac ctcttcgagc
     6841 ggcgcgacgc ccgcaggccg tcctcccagg aacctcccga cccccctcca cgagagaccg
     6901 tacgacgctg gcgcgaaacc ctgatcgcag gggaggatct cggagaggcc cgcacgctgg
     6961 agatgctcac agactacggg gtgcccgtag cgcgtaccgc cgcggcgacc gacatcgaag
     7021 gtgtcctggc cgccgcccgg cgcctcggct acccggtcgc cctgaagacg gccgcagctt
     7081 cccacaagac ggagctcggc ggggtgacgc tcggtattcg ggacgaagac ggcctgaccg
     7141 gggcttacag ggagatgtcc tcacacctcg gcccctccgt cacggtacag gagatggtgg
     7201 atccgggcgt cgagatggcg ctcgggatgg tacacgatcc ccagttcggt ccgctcgtga
     7261 tggtcgccgc cggcggcgta cttatcgaga cgctcggcga caggcgcctg gccttgccgc
     7321 ccctggacga ggcgagggcc tcgaagctta tcgacaggct cgacgtccgt ccgctgctcg
     7381 atggggtcag gggcgcacca ccggcggatg tgaactctct ggccaggact ctttcgcggc
     7441 tctccctgct ggccggagac ctgggggatc tcatcggcgc tctcgacgtc aaccctgtta
     7501 tcgtcggtcc gcgcgggtgc gtggccgtcg acgccctcgt agagcaatca gctaacggct
     7561 ttcagcaaga aaaagctgaa agccgttagc tgactgctgg ttggtccggt acgtgggaga
     7621 tcccaccctc caggtaccct cgggccaccg ttgccacgaa cttgcccgac gtcgactcgt
     7681 ccggtgagag cgggccctgc cggtaaccgc gcgaggaagc gccactctgt acgccctcac
     7741 aagcccccca atcctgcccg ttggtgacat cccagaactc gctggcgtac gaaggatcga
     7801 agccctcgga ctcgaccgcc ttcttcgaga acagccactc gcactccacg gctgtccttc
     7861 cgggggcgag gggtctcagg cggtgggtca tcacgtagtc cgggtggagg cttataagga
     7921 ggttcgggaa gagctggtag tagtacactt cccgctcctg gagcgccgtc aggtgcggca
     7981 gcttcggggc gaagctctcg ccggtcagcg acatggtctc ggcgtgatcc tcgagctcca
     8041 tggagccgcc gaaccagatc ccccgcgcct cgtggtcctc gccgctcttg ggcgggctca
     8101 ccttgcagag ctcgggatgt atggaggagc agtggtaaca ctcgtggtag ttctccacca
     8161 agatcttcca gttagccgcc acctcgtaac tgtgcctggc ggccgtcacg agctcacccg
     8221 agcggtggtt cccgatcatc tcccccaaac tgcccacgtg ctcctcgaaa ggaggtgcct
     8281 cgccgctcgc gttcgcgaac agccacccct cccactccgc gaccctgacc ggcacgagcg
     8341 agtagtcaga cttgtcgaac ccgggtagct ggccgaaacc aggagccccc tgcagactcc
     8401 catccagccc gtaagcccag gcatgataag ggcagcggat gaacctgccg ctagacgact
     8461 cgcctacctc ggcgagcacg tgcccgcggt ggcggcacac gttgtagaag gcccgcaagt
     8521 tccccccctc gtcccgcgca agaagcgccg actcgtggcc gagcgcaacc gcacaccggt
     8581 cccccacacc gcgcaaatcc tccgaacggc ccacgcatac ccaactctcg tcgaagaagt
     8641 gctccatctc ccaatcgagc accgcatcgt ccgtgtaagc ctcggcgggc aaactcatct
     8701 gcccctcaga gaccaacgac gaagccaaca actcgcggtc aataggcgtc atgagcactc
     8761 cctctcgaac ccgtccgtta cacacttctc tacggcctcg ccaccagcgt gctgatctgc
     8821 agcatgccgg ttgccccagc ccgccgattt cagtataatc tattttgaat gacggttcaa
     8881 gaatgcatcc gtattgggtt atcgtctccg gtcctccggg acacgggctt ccgaacatgt
     8941 tccggagagc caggtagtgg gaggtgccgc atgcgggcgg cagcggcgaa ctcgtcgacg
     9001 cgacagcgta ttcctgaact gcggttcaag cgtggcgttc gggctgcctg gcacccgtag
     9061 ataggatttt cgtaggggcg ggcgaaggat cgtgaggagg agttgggcat gaggatctac
     9121 gatgttgtcg tggttggcgg tggaaacctc gggttgtgga cggcgcaccg gctggcgcgt
     9181 cgtggtttcg ggcggatagc ggtgttggag cggggttggg ccgggggcgg ggcgacgagc
     9241 cgttcggccg gcgtggtacg ccaacagggc ggttccgaga cggctgcgaa gctgggcaag
     9301 ctatccaggg agttgtattt gcgactcggg gaagagctgg gcctggactc tggttttcgg
     9361 gagacgggat acttcatcgt cgccgagacg gaggaggaga aggaagggtt cttgcggctg
     9421 gtcgaggtgc ggctggcggc gggcgtcgag aacgagtggg tggagcccga ggagggcaga
     9481 cggcgttttc ccgacctgaa ctggaaccgt tttgtcgggg cgacttacac ggccgacgat
     9541 gggtacgtcc atccgccggt ggcggcccgc aatgccacct gcgcggttct tggtgacgat
     9601 acggtcgacc tcttcgagat gtgcgaggtg acgggcgttg gccagaaggg cgaccactac
     9661 tctgtggaga gtgcatgcgg caccttcgag gcagagcggg tcgtggacgc gggcgggccg
     9721 cggggagtgc gggacgtcgg cggcatggtc ggggtggagg tccccgtgat ggcggtgcgg
     9781 caccagatcg tgagcttccc ttacttgccc gaggggttcg agcatccgtt cccgctggtg
     9841 ttcaacgtgg gcaagggtta ctactggcgg cccgaggagc agggtgtctt gctcggcatg
     9901 agcaaccccg tcgacgaggc tgacgagacg ggacgctacc agatcgactt cgactgggac
     9961 tactacgcca gaatgcgccc tgactgggag tctgcgcacc cggcgctaag gggtttgccg
    10021 gtagcgcggg cgtgggcggc ctcgatcgac tacacgcctg accacctccc cataatcgat
    10081 gagccgatgg agggtttcta cgtccttgcc gcagggggac acgggatgat gtgggggcct
    10141 gcgctcgggg agaagatggc cggccttatc tctgaaggca gggtcgaaga cttgcccgag
    10201 gaggacatag agctcgcgcg gttctcgcgg gaaagggacc cgaacaggat cgaggacacc
    10261 atcgccctgc ccttccccaa agagtagaag aggagagagc tgtgacgacg gcgccacaga
    10321 tgacggcgga gatcccgcaa gaagtaaagg cgttgaaggc ccgcatcgcc gagttcgtaa
    10381 gggaggagct tcatccgttc gaggcggaga tcgccgagcg cggggagatc gacgaggaga
    10441 agctgcgggc gctacgcaac aaggcgcgcc gggccgggtt cagcaacctc aacatgcccg
    10501 ctgagtacgg cggtatggac ctgtccatgg tctcgctggt ggcgctggag gaggagtcgg
    10561 gcaaggttac gaacggcctc ggatacatgg tcgccgacag gggaccgcgg gagctcctcg
    10621 agctggccag cgccgaacag gtagagcgct acgtcatgcc cgtcttgcgg ggcgagacgc
    10681 gggaggcgtg ggccatcacc gaacccgagg ccggctcgga cataaaggac ataaagacca
    10741 ccgccaaaag agatggtggc gggtgggtcc tgaacgggga gaagtggttc gtgaccggag
    10801 gtgagcgggc cggcttcttt atcgtgctgg cgtgggccgg cgaggagcag gcgtcctttc
    10861 tcgtggagcg tgagacgccc ggactggagt tcgtgcgcac gccggtcttt atgcacgatc
    10921 cctacgcctc caagcacgtc gagttgcggc tgaaggactg ccgggtccct gagggggcgc
    10981 ggctgggaag cgggggagat gaggggacca agcgctggtt ctcgctggag aggctgatga
    11041 tagcggcgcg ttgttgcggg gcggccgaga ggctcataga tctggcccgt tcgtggtcgc
    11101 tggagcggca ggcgtcaggg aagaagatct ccgagtacca ggcgatccag ttcatgctgg
    11161 ccgactcggt gaccgaactg aacgccgcca ggcttatggt gaaccacgtg gccaggatgt
    11221 gggaggtgga agccccgagc atcctgcacg ggaagacggc gatggcgaag ctgttcgcct
    11281 ccgagatggc cggcagggtg gccgaccggg cggtccaggt cttcggcggc cgcgggtaca
    11341 tgaccgagaa ccctgccgaa cggtactacc gcgagctcag ggtcgaccgc atctgggaag
    11401 gcacgagcga gatacagcgt tcgatcatcg cccgcgggtt gctgaagcgg ggccgagagg
    11461 cgtacacgat ggactggtag gaaggctaca cagtctctct aacacttatg gcgaggtacg
    11521 gctaaacgac cgtgactagg tacgctccgc gagcgcttcg atctctgccg cgagtcgtcg
    11581 cagcgactcg cggagttcgg gcggctcgcg caccacgaag gggaatgaga gccctgcgag
    11641 cacccgcgcc atccacatga ggtcggaggt cgaagagcgc ataaggacac cctcgggcgc
    11701 ctcctgaaga gagacgccca tcttggggag ttgcccgcgc gcctctcccg gtgtggtctc
    11761 cagcaacacc tctacggacc agcggtcctc ccgcatgtcc gcgagcgcct cgagaacgcc
    11821 ctggggtgta tcgaagcccg gtgggagtac gaacgcttcg tcgagcatct ccaccgagag
    11881 tacccgatcc aacctgaaca gacgtctccc ctcccgcagg tgacagtaac cgaaggtgta
    11941 ccagaaggcg tccccctgga cgacggcgta gggatccaca accctccgcg tctcccctgc
    12001 gtctcccgcc cggtagcgca tccacacccg cttgcgctcc cgcacggcct cgctcaaggt
    12061 cacgacgacc tggcccgcag ggagacgcgc cggagccgag gctgcgggga cgacgacctc
    12121 ctcgaaggtc cgcacccgct cgcgcaactc ctcgggcatg acgcgctcca gcttcgccct
    12181 ggcgccctcg acggcggggg cggccccgga tagaccgagc ctgcgggccg agagcagccc
    12241 cagggccagc gcgagagcct cctcgtccgt gaacatcata ggcggca
//
LOCUS       DATMVF010000224         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_441193, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000224 DATMVF010000000
VERSION     DATMVF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441193"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VN178_11655"
     CDS             complement(<1..493)
                     /locus_tag="VN178_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81668.1"
                     /translation="MLLTAVYGVLVIGAVCLAALAGLELVQRLVPAASRQPHNDVAGF
                     IYAALGVIYAVLLALVVIAVWEEFQVANETVEQEANAAADIFWLADRLPEPRGTHLQE
                     LVRSYAEEVVHREWPLMEQGQAPLMTQTRGASSGWTIIDDIRQNLQNFEPRTKAEEQL
                     YAEG"
     gene            1039..1869
                     /locus_tag="VN178_11660"
     CDS             1039..1869
                     /locus_tag="VN178_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HWS81669.1"
                     /translation="MFTFLLRPLAPAGVARRVPHHYDAYMGIKGLLLDLDGTLYVGEE
                     PVAGAREALGALEASGLALRYVTNTTRMPRRAVVERMRTLGFLVEEDEVFTPAWAASR
                     LIGDRSCLALVEESLYEDLGRVRLTKDSPEVVLVGDLGEGFTYARLDAAFRCLVDGAE
                     LVALQKNRYWQNKGRLSLDAGPFVAALEYASEVRAIVVGKPEESFFRIALEDMGFEAH
                     EVAMVGDDAEADVAGAKNAGLSGIQVRTGKWRPGGDAAEADLVLDSVAALPLALEGER
                     "
     gene            1866..2264
                     /locus_tag="VN178_11665"
     CDS             1866..2264
                     /locus_tag="VN178_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWS81670.1"
                     /translation="MRISDIPVETGERYELVSITDEVRRAVRDAGVEEGLCVISSTHT
                     TAAVTVNEDYDRDVPRDLASACRSFLEALDVRFEHAEGNSDSHLLSSLFGQSQTLLVR
                     DGELDLGRWQGVFLAEFDGPRSRRVRVAVS"
     gene            2379..3767
                     /locus_tag="VN178_11670"
     CDS             2379..3767
                     /locus_tag="VN178_11670"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS81671.1"
                     /translation="MNGLVKGGLALGGLVLGLGALRRALNPTPRYAPWEKPPYGEFEK
                     KVLIVGGGFGGYTAATDLCKLVEKRDDVGVLVIARDNFFTFWPMVPGIVSSDIDARNV
                     AQPLRRALILAGASFRRAKVKGVDTNKKVVEADGGLEFPYDHLIISLGGQPNFFGIPG
                     VEEHSLTMRGVEDAVRVRNRVIERFEEVSLIRDEVPESKLTFVVIGGGATGVEVASEI
                     HTLVHENLAPDYPNIDPYRVRIYLLEALPNILPELDPALRRAARARLLNERIEVMTGA
                     MAEEITGDCVKLRGGKQIETENVVWTAGNRPNQTIQNLGLPYDERTGIKVDLSLRVEG
                     RSDVWAVGDCAAVQDVHKEGAIVPPTAQAAIQQGHLVARNVLKTIDGEGDLGEFEYKP
                     LGQLVELGSDFAVNEVMGVRFTGIIAAVFWRLAYLVRLNSPQSKVRVAADWIFGLLLR
                     PAVTQIRGSYEE"
     gene            3863..>4249
                     /gene="pgsA"
                     /locus_tag="VN178_11675"
     CDS             3863..>4249
                     /gene="pgsA"
                     /locus_tag="VN178_11675"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWS81672.1"
                     /translation="MSFANQLTLIRLVAVVPVMVALYLPFEWARPVAVVLYVGAILTD
                     YFDGIIARRNNEVTGFGKLMDSIADKALIVSLFFALVGEGSMAAWMAAIMVIREFAVT
                     GLRMVALEAGEVIAANRWGKAKMNAQS"
BASE COUNT          803 a         1236 c         1415 g          795 t
ORIGIN      
        1 gcccttccgc gtaaagctgt tcctcggcct ttgtgcgcgg ctcgaagttc tgtaagttct
       61 gccggatgtc gtcgatgatg gtccacccac tggaagcccc ccgtgtctgc gtcatcaagg
      121 gtgcttgccc ctgctccatc aagggccact ccctgtggac cacctcttcg gcgtaggatc
      181 ggacgagctc ctggaggtgg gtgccccggg gctccggaag ccggtcggcc agccagaaga
      241 tgtcggccgc cgcgttggcc tcctgctcga ccgtctcatt ggccacctgg aattcttccc
      301 acacggcgat gaccacgagc gccagaagca cggcgtagat cacgcccaat gccgcgtaga
      361 tgaacccagc gacgtcgtta tggggctggc gcgaggcggc ggggacgagt cgctggacca
      421 actccagtcc ggcgagcgcg gcgaggcaaa ctgccccgat cacgagcaca ccatatactg
      481 ctgtgagcag cacgcaggac ctccccatct gtaatgaccg gcccaagaat aacacctgag
      541 caaggggctc gaatccaacg cgcccacagg tcgcgggccg gcgctatgcg ggatcctgga
      601 tctggacctc ggagaaactg cattacggag tatttcggtg aacaaagtcg tctcggtgtc
      661 cgcgcgcagc ggaccgtatc gagatagtcc gttcgaggaa ttccctcgct agaagggtgg
      721 gcatctcttc agggctcggg aaaggaagga taatcttgtt gggacaatcg ccgagccccg
      781 aaaagacgca gtatggcgct cgtttctgag aacagcctga gagaatctac ggggatcgag
      841 tgcagttcgc gcagctcatc ccttccatgc actcggggta tgacagcggg tagcagaagg
      901 attgagctcc tgcaacccgc gctctcagca cacagctata agccttcagc cttttgttct
      961 tgctgaaagc ttatagctta tagctcctcg tgtttgtggg gccttctgct cgggtgtgta
     1021 accgggatca ttgccatcgt gtttacgttc ctcttgcgac cgctcgcgcc ggcaggtgtg
     1081 gcccggcgcg ttccgcacca ctatgatgcg tacatgggca taaaaggtct tctgttggat
     1141 ctcgatggaa cgctgtatgt cggagaagag cccgtagctg gggcaagaga ggccctaggg
     1201 gccctggagg cttcagggct tgcgctcagg tacgtcacga acacgaccag gatgccgcgc
     1261 cgcgcggtgg tcgagaggat gcggacgctc gggttcctgg tcgaggagga tgaggtcttc
     1321 acaccggcgt gggctgcctc gcgcctgatc ggggacagga gctgcctcgc cctggtggag
     1381 gagtctctgt acgaagacct tggtagggtc cgtctcacga aagattcacc cgaagtcgtg
     1441 ttggtcggag atctgggaga aggctttacc tacgcgcgcc tcgacgccgc atttcggtgc
     1501 cttgtggatg gggctgaact cgtagcgctg cagaagaacc gctactggca gaacaagggt
     1561 cgactctcgc tagacgcggg acctttcgtc gcggcgctcg agtatgcgag cgaggtaaga
     1621 gccatcgtag tcggcaagcc ggaggagagc ttcttccgga tcgccctcga agatatggga
     1681 ttcgaagccc acgaagtagc catggtcggc gacgacgccg aggccgacgt cgcaggcgcg
     1741 aagaatgccg gcctctcggg tatacaggtc aggaccggaa aatggcggcc cggtggcgat
     1801 gcggctgagg cggatctcgt actcgacagt gttgccgctc tgccactggc tctggaagga
     1861 gagagatgag gatcagcgac ataccggtcg agacgggcga gcgttacgag ttggtctcga
     1921 tcacggacga ggtgaggcgg gcggtaaggg atgccggcgt cgaggaaggt ctgtgcgtaa
     1981 tctcatcgac gcacactacc gcggccgtga cggtcaacga ggattacgac agagatgtgc
     2041 cgagggacct tgcctccgcc tgcaggtctt tcctcgaagc tctggacgta cgcttcgagc
     2101 acgccgaggg caacagcgat tcccacttgt tgagcagtct cttcggacag tcgcagactc
     2161 tactggtgag agatggagag ttggacctcg ggcgttggca gggcgttttc ctggccgagt
     2221 tcgacggacc gcgcagcagg agggtcaggg tggcggtctc ctagccggcc cttacggttt
     2281 agttgagacc caactttagg taagatgggt ctgcggatag tagtgtcgcg gactcggatg
     2341 ccgtggccgg ccgtaatcag tcttcgcggg aggcttgtat gaacggattg gtcaagggcg
     2401 ggctggcgct cggcgggttg gttttggggc tcggtgccct gaggcgggcg ctcaacccta
     2461 caccccgcta cgcaccctgg gagaagccac cctacggcga gttcgagaag aaggttctga
     2521 tcgtcggagg agggttcggg gggtacacgg cggcgacgga cctgtgcaag ctggtcgaga
     2581 agcgcgatga cgtcggagtt ctggtcatag cgcgcgacaa cttctttacc ttctggccga
     2641 tggtgcctgg gatcgtcagc agcgacatcg acgcgcgcaa cgtcgcccag cccttgaggc
     2701 gcgccctcat cctcgccggg gcaagcttca ggcgggccaa ggtcaaaggc gtagacacga
     2761 acaagaaggt cgtcgaggcc gacggaggtc tcgagttccc ttacgatcat ctcataatct
     2821 cactgggcgg acagccaaac tttttcggca tccccggtgt ggaggagcac agcttgacca
     2881 tgaggggggt cgaagacgcc gtgcgcgtgc gcaaccgcgt catagagcgc ttcgaggaag
     2941 tctccctcat ccgcgacgag gtgcccgagt ccaagctcac tttcgtggta atcggtggtg
     3001 gggctaccgg ggtcgaggtg gcctccgaga tccacactct cgtccacgag aacctcgcgc
     3061 ccgactaccc gaacatcgac ccttaccgcg tcaggatcta cctcctggag gccctgccca
     3121 acatcctgcc cgagctcgac cccgccctgc ggcgcgccgc ccgagccagg cttctcaacg
     3181 agcgcatcga ggttatgacg ggcgccatgg ccgaggagat cacaggtgat tgcgtcaagc
     3241 tgaggggcgg caagcagatc gagacggaga acgtggtctg gaccgcgggc aaccgcccga
     3301 accagacgat acagaacctc gggctgccct acgacgagcg gacgggcata aaggtagatc
     3361 tatctctgcg ggtggagggc cgcagcgacg tctgggccgt cggggactgt gcggccgtcc
     3421 aggacgttca caaggagggc gccatcgtcc cgccgaccgc gcaggccgcg atccagcagg
     3481 ggcacctggt cgcccgtaac gtgctcaaga ccatcgatgg tgagggggac ctcggagaat
     3541 tcgagtacaa gccgctcggg cagctcgtcg agctgggaag cgacttcgcc gtcaacgagg
     3601 tgatgggcgt aagattcacc gggattatcg cggccgtttt ctggcggctg gcctacctcg
     3661 tccgcctgaa cagcccccag agcaaggtgc gggtcgctgc ggactggatc ttcggtctgt
     3721 tactgcgacc cgccgtcacc cagattcggg gctcttacga ggaatagcca agcccttgag
     3781 acgcggccac aaaggtgata cgttgaatgt ggctagagga ggagaagatg cagcttagag
     3841 ggcttgagag acgggggctc ccttgagctt cgcaaaccag ctcaccctga tccggctcgt
     3901 cgccgtcgtg cccgtgatgg tcgcgctgta tctgcccttc gagtgggcgc gtcccgtcgc
     3961 cgtcgttctt tacgtgggtg cgatcctgac cgactacttc gacgggatca tcgcccgccg
     4021 caacaacgag gttacgggct tcggcaaact gatggactcc atagccgaca aggccctcat
     4081 cgtctcactt ttcttcgcgc tcgtggggga gggctcgatg gccgcgtgga tggcagcgat
     4141 catggttatc cgggagttcg ccgttacggg gctcaggatg gtcgccctcg aggcggggga
     4201 ggtcatcgcc gcgaaccgct ggggcaaggc gaagatgaac gcccagagc
//
LOCUS       DATMVF010000225        10596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_25570, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000225 DATMVF010000000
VERSION     DATMVF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10596
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25570"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VN178_11680"
     CDS             complement(<1..268)
                     /locus_tag="VN178_11680"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWS81673.1"
                     /translation="MQREKPTILVTGGAGFIGGNFVLGTVGDYRVVNLDALTYAGNPE
                     TLASVWGHPDHIFVQGRIGDRELVDRLLAEHRPSAIINFAAETHV"
     gene            533..1600
                     /gene="thrC"
                     /locus_tag="VN178_11685"
     CDS             533..1600
                     /gene="thrC"
                     /locus_tag="VN178_11685"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWS81674.1"
                     /translation="MSVQARVSRQGVIRRYREWLPEIPDEAIATIGEGETPLIEATRI
                     SERLSARLLLKFEGMNPTASFKDRGMTVAMSRASATGSRACVCASTGNTAASAAAYAA
                     RAGMECFVVVPAGKIALGKAVQVLAHGARIVQIEGNFDAALKLSNRIAHELGDVTLVN
                     SVNPDRIEGQKTAAFEVAEALGDPPDALALPVGNAGNITSYWKGFCEWRGAGHADSAP
                     RMLGFQAEGASPLVAGHDFESPETVASAIRIGSPASKEGALSAGRESGGLIESVTDGE
                     ILAAQEMLAQEEGVFCEPASAAGIAGLLKLASEGRGPEGTVVSVLTGHGLKDPDVILG
                     RVQLPDPVPATFEAVAGSMER"
     gene            1606..2538
                     /gene="thrB"
                     /locus_tag="VN178_11690"
     CDS             1606..2538
                     /gene="thrB"
                     /locus_tag="VN178_11690"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWS81675.1"
                     /translation="MISVRVPATSANLGPGYDAVGLALSLSMRIGLDRSPHPEIEIRG
                     TGADLIPRGPEHPAYRAARFVAELVGESDAQFRLVQENSIPPTRGLGGSAAALVGGAV
                     AANDLFGGQIAAPDLLNIVCELDGHPDNAAPALLGGLVIGTLTPVGVSAVRLEPKDLK
                     VVVAVPDFAVSTTAARRALPDSVPHRDAAFNVGRSGLLLGALATGEYHLLRVAMQDRL
                     HQPYRSHLIPGLEDVIEVALKTGAYGACLSGSGPTILAFAPQSRAGEIASAMKATFQA
                     RGVVADSWALDVDLAGARVEPPEEVSALIPAPVL"
     gene            complement(2629..3882)
                     /locus_tag="VN178_11695"
     CDS             complement(2629..3882)
                     /locus_tag="VN178_11695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWS81676.1"
                     /translation="MAKNAGTHGSSKGATIIDRRRFLKQTALATAVVSVPYLAGKTVA
                     FGKEGTTSGQGTKGADAALDRALKELVAMEDGPPGVIAVVQRGKHRKVHTFGVRNIKS
                     GLPIRIDDRMRIASVAKAFSGAVALSLVSKGALSLNDTIGKLLSGLPEPPPDAWAEIT
                     LRQLLNHTSGLPEILEDLEFLDAFGASPKKAPPPEDLLTFLYNNDPPLRFEPGSKYQY
                     SNSDNIAVALMVEAATGTSYEDLLRELVYKPLGLRRTSLPRGPNLRKPFIHGYDTTQD
                     PPEDVSEDFAAGWAWASGGIVSTPADLNTFIRGYVGGKLFDERTQAKQRRVIEGGSSE
                     PPGPGKNSAGLAIFRYETRCGTVWGHTGNTVGYTQFAAASADGRRSVTVSINALHTLV
                     AGNQDVFRALRRAEVKAVCAALAGS"
     gene            4336..6054
                     /gene="ilvB"
                     /locus_tag="VN178_11700"
     CDS             4336..6054
                     /gene="ilvB"
                     /locus_tag="VN178_11700"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HWS81677.1"
                     /translation="MGQTVENTALNIEYKPRTGSEAMCEALLDEGVEYLFGYPGGAIM
                     PFYHALPDYPLKHILTRHEQAAAHAADGYARATGGVGVCVATSGPGATNLVTGLATAQ
                     MDSVPLVAITGQVGRPAMGKDSFQETNVMGMTLPFTKHSFLVEDPDELYPAMRRAFEI
                     ARSGRPGPVLVDVPKDVQLAATGDNGYRRLREAPPPVESPGLERAAMLLRNAKRPLIL
                     AGHGVTLGSAEKELRLLAERTGIPVVTTLLGISAFPDDHPLYLGWPGMHGQASVNRAI
                     DRSDLLFAVGMRFDDRITGAAHKFAPRAKIIHIDVDPSELGKVIKPTVGIAADAKAAM
                     RSILGHLDNAPDSGEGCKQWREELATSQEPERRRKESARGEYGPIQVMEAIKSVAGPD
                     LRLVTDVGQHQMWAAQFFEFTKANSHITSGGLGTMGFALPAAMGVAFAYPEDPVWVIA
                     GDGGIQMNIQELATIRDFGLNIKVAVLNNGYLGMVRQWQQFFHNRRYSETPITGPDYE
                     QLSASYGLAGRTVRDGDDVEAAVRWAEEQEGCTILDFHIDPEANVYPMIPSGMSVNEM
                     IEEDGA"
     gene            6047..6505
                     /locus_tag="VN178_11705"
     CDS             6047..6505
                     /locus_tag="VN178_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81678.1"
                     /translation="MPESATGVALNTIEVRFTGGMLALNRLLMTLQNKRMPVAGFTLG
                     GDSDGMRATILLDCPPEPALRYTALISALEDVSEAGPAKTIEVALVETSQDWREPAER
                     SGVEAHEDGGTVLASGEPEKVEAFLAALGGGVEDVVRLGPVARPDAGGGV"
     gene            6506..7528
                     /gene="ilvC"
                     /locus_tag="VN178_11710"
     CDS             6506..7528
                     /gene="ilvC"
                     /locus_tag="VN178_11710"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWS81679.1"
                     /translation="MARVYREGDIGNEVTSRRVAILGYGSQGHAHALNLKESGCDVRV
                     GLYEGSKSWPKAERDGLQVQTIADAVRGADVVMMLLPDEKQPAVFEAEVGPSLSEGDL
                     MLFAHGFNIHFNQIVVPPEVDLGLVAPKGPGHVLRRLYVEGKGMPSLFAVENDASGQA
                     RDVILSYARAIGSGRAGIIETTFAEETETDLFGEQAVLCGGLTALLTAGFETLVEAGY
                     QPELAYYECVNELKLIVDLIYEGGLAGMRYSISNTAEYGDYTAGPKIIDEAAKERMRS
                     ILADIQSGKWAKDWVLENQAGGSSFLAMRRREAGSQVEKVGQELRALAAEGIETPAGS
                     AGGENL"
     gene            7525..8481
                     /locus_tag="VN178_11715"
     CDS             7525..8481
                     /locus_tag="VN178_11715"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWS81680.1"
                     /translation="MSRKAFAASDAEWAYHGGELVRVGDIRLSPATHALNYGTGVFEG
                     IRAYWSERRGTLQVLKMREHYERFERSCRLLRIELPNTVDELCDVTLEILRRNAPRQD
                     TYVRPLAYKAAESVGVNLKGETELSIFTVPMGNYVELTGLRVCVSSWRRTPDNAIPAR
                     AKCTGSYVNTALAVDEAHMSGYDDAIFLTQDGQVSEASAANIFLVRKGRLITPPVTAD
                     ILEGITRDAVMELASKELGMPVIERAVDRTELYAADEVFLSGTGFQIAPVVEVDDRPV
                     GTGSIGPVAERLQELYFKAARGEWEEYADWTVAVEVAERAAG"
     gene            8665..10206
                     /locus_tag="VN178_11720"
     CDS             8665..10206
                     /locus_tag="VN178_11720"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWS81681.1"
                     /translation="MRRVEIFDTTLRDGEQSPGISLSVEEKVEIARQLARLKVDVIEA
                     GFPITSEGDFESVSRIASEVKGPRIAGLARIHEQDIVRAWEAVQWSQRPRIHTFVGTS
                     DLHIEHQMRSNQEDILRRAGEAVVFAKSLCENVEFSPMDATRTDIGYLSEIVAAAVEA
                     GADVVNIADTVGYTTPVEFADFLKELQERVPALQERVLSVHCHDDLGMAVANSLAGVE
                     VGAGQVEVAVNGIGERAGNASLEEVVMALATRSDFYGARVGVETRQLANTSRMVSNIT
                     GYEVPPNKAIVGRNAFLHESGIHQDGVLKDRRTFEIMTKEDIGLEGTNIFLGKHSGRH
                     ALKDALEELGYYVEGDVLKRAFTRFKEIADHKKTVTAADLEAIAADEVGSFEGKFVLE
                     SFRVVAATGRQSRAAVTISHAEQGTFEAEAEGEGPVDAVFRAIDAATNIKGRLTDFRI
                     DAVTGGKDALGEVRVSVEFEGGDYAGRGLSQDVVEAAARAYVRAVNVYTAGMVGARWD
                     AQVAP"
     gene            10263..>10596
                     /locus_tag="VN178_11725"
     CDS             10263..>10596
                     /locus_tag="VN178_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81682.1"
                     /translation="MPVLYAPRLAGKARELSGYLTQGYRALGRILTVEPPGIVAYLVA
                     DEAWADAPRDNARPYPPGLPYFTRSVEPPVLVLPEELSTVFRPRTAATLPLTVWHELA
                     HAFLLGREV"
BASE COUNT         1905 a         3392 c         3520 g         1779 t
ORIGIN      
        1 cgacgtgcgt ctcggcggcg aagttgatga tggccgacgg acgatgctcg gcgagcaggc
       61 ggtccaccag ctccctatcc ccgatcctcc cctgtacgaa gatgtggtcg gggtgacccc
      121 agaccgaggc cagtgtctcc gggttgccgg cgtaggtcag ggcgtctaga ttgaccaccc
      181 tgtagtctcc tactgtacca aggacgaagt tgccccctat aaagccggcg ccgccggtaa
      241 cgaggatggt tggtttctct ctctgcaatg cgctcctttg gttttagctg tcagctatca
      301 gccttcagct ttcagcttct tgcctctggc tgactgctga ccgctgactg ctgatagctc
      361 aatacgtagc ctactaaacg ggtcgtgggg tcgccacatt cttgaaacgc tgcgtcggtg
      421 cgtgatactt ttcttacgct gtggtttgtg gcttctacac ttttgttcgc tgctagattc
      481 cgggttcgta gagtcgaacc ggcccgagtt cgagcgaaag gctaggcttg tattgagcgt
      541 tcaggcgcgg gtgtcgagac agggcgttat ccggaggtac cgtgagtggc tgccagagat
      601 tccggatgag gccatagcca ccataggtga gggggagacg cccctgatcg aggccacgag
      661 gatctcggag cgccttagcg cccgcctgtt gttgaagttc gaaggtatga acccgacggc
      721 ctcgttcaag gaccgcggca tgacggtggc catgagcagg gcctccgcaa cggggagccg
      781 cgcctgcgtc tgcgcctcga cggggaacac ggcggcatcg gccgcggcct acgcggcgcg
      841 cgccggcatg gagtgcttcg tcgtcgtgcc cgccggcaag atcgcgctgg gcaaggccgt
      901 acaagtcctc gcgcacgggg cgcggatagt ccagatcgaa ggcaacttcg acgctgccct
      961 caagctctcc aatagaatcg cccacgagct cggcgacgtc acgctggtca actcggtgaa
     1021 ccccgaccgt atagagggcc agaagacagc ggccttcgag gtcgcggaag ccctcggaga
     1081 tccgcccgac gcgctggcat tgcctgtggg gaacgcgggc aacatcacct cgtactggaa
     1141 gggtttctgt gagtggcgag gggcggggca cgccgactcg gccccccgca tgctcggctt
     1201 ccaggccgaa ggggcctcac cgctggtggc cggccacgac ttcgagagcc ccgagacggt
     1261 cgcgagcgcc atcaggatag gttcgcccgc gagcaaggag ggggcgctct cggccgggcg
     1321 cgagtccggt ggcctcatag agagcgttac ggacggggag atcctggccg cccaggagat
     1381 gctggcccag gaagagggtg tcttctgcga gccagcctcc gcggcaggca tagcaggact
     1441 cttgaagctc gcctccgaag gcagggggcc tgaaggtacg gtcgttagtg ttcttaccgg
     1501 gcacgggctc aaggaccccg atgtgatcct tggccgcgtg caactccccg accccgtccc
     1561 ggcaacgttc gaggccgtcg cggggtccat ggagaggtag gcgcggtgat ctccgtccgc
     1621 gtccccgcca cctccgcgaa cctcggacct ggctacgacg cggtggggct cgcgctctcc
     1681 ttgagcatga ggatcgggtt ggacaggtcg ccccatcccg agatagagat acggggcacg
     1741 ggcgccgacc tcatcccgcg cgggcccgag caccccgcct acagggcggc ccgctttgtg
     1801 gccgaactcg tcggcgagtc agacgcccag ttccgactag tgcaggagaa ctctatcccg
     1861 ccgactcggg gtctgggcgg gagcgcggcc gcgctcgtcg gcggggccgt cgcagccaac
     1921 gacctgttcg ggggtcagat cgccgccccc gacctgctca acatcgtctg cgagctcgac
     1981 ggccaccccg acaacgcggc ccccgcgctc ctcggcgggc tggtgatcgg aaccctcacc
     2041 cccgtcggcg tgagcgcagt acgcctcgaa ccgaaggacc tgaaggtcgt cgtcgccgtc
     2101 cccgatttcg ccgtctccac cacggcggcc cgccgcgcgc tcccagactc ggtcccccac
     2161 cgcgacgccg ccttcaacgt cggccgctcc ggtctgctgc taggtgccct cgcgacgggc
     2221 gagtaccacc tcttgcgcgt cgccatgcag gacaggctcc accaacccta ccgctcccac
     2281 ctcatcccag gattggagga cgttatagag gttgccctaa agaccggtgc ctacggcgca
     2341 tgcctctccg gctcaggccc taccatcctc gccttcgcac cccagagcag agccggggag
     2401 atagcctcag ccatgaaagc gacattccag gcgcgaggtg tcgtggcgga ttcctgggcg
     2461 ctcgacgttg acctcgcagg ggcccgcgta gagcccccgg aagaggtctc cgccctgatt
     2521 cccgcgcccg tgctctagag ctgactatag aaaggtgcgt ccccagcgtc cggcgcagac
     2581 ttgaccctcc tcaggcggct gcctacagag cttctccggg gagatggcct aactgccggc
     2641 cagtgcggca cacacggcct tgacctccgc acgcctcagc gccctgaaga cgtcctgatt
     2701 gccggccacc agggtgtgta gtgcgttgat ggagaccgtc accgagcgcc tcccgtctgc
     2761 gctggcggcg gcgaactggg tgtagccgac ggtgttgccc gtgtgccccc acaccgtccc
     2821 gcaacgcgtc tcgtagcgga agatggccag cccagccgag ttcttgccgg gaccgggcgg
     2881 ttcggagctt cctccctcga tgacccgtct ctgctttgcc tgcgtccgct cgtcgaagag
     2941 cttgccaccc acgtaaccgc ggatgaaggt atttaggtcg gcgggggtag agactatgcc
     3001 ccccgaagcc caggcccacc cggcggcaaa gtcctcgctt acatcttcgg gcgggtcttg
     3061 ggtggtgtcg tagccgtgga tgaagggctt cctcaggttg gggccgcggg gcaggctagt
     3121 ccttctcagc ccaagaggct tgtagaccag ctccctgagc aggtcctcgt agctcgtgcc
     3181 tgtggccgcc tcgaccatca gcgccacggc tatgttatcc gagttggaat actggtactt
     3241 ggaaccaggt tcgaagcgca agggcggatc attgttatac aggaacgtaa gcagatcttc
     3301 cggcgggggc gctttcttgg ggctggcccc gaacgcatct agaaattcta ggtcctctaa
     3361 gatttctggt aagccgctgg tgtggtttag gagctgcctg agggtaatct cagcccaagc
     3421 gtcgggaggc ggctctggca agcctgagag gagctttccg atggtgtcgt tcaaggagag
     3481 tgcccccttg ctcaccaaag agagtgcgac ggcaccgctg aaggccttgg cgacgctcgc
     3541 aatgcgcatg cggtcgtcga tccttatggg cagcccgctc tttatattgc ggaccccgaa
     3601 ggtgtgtacc ttgcggtgct tgccgcgctg cacgacggcg atgaccccgg gagggccgtc
     3661 ttccatggcg acgagctcct ttagcgcgcg atccagcgcg gcgtccgcac ctttggtccc
     3721 ttggcctgag gtggtgccct ccttgccgaa ggccacggtc ttgccggcca ggtaagggac
     3781 ggagaccacc gcagttgcca gggccgtctg tttgaggaac cgcctcctgt cgatgatcgt
     3841 cgctcctttg gatgacccat gcgttcctgc gtttttcgcc attctcttct cccttctccc
     3901 acgcagaact cgacaagcat tatgcgctta agtgggccga ctggcaaact agcctcgaga
     3961 tagcccggaa agcttgctgt ccttgtggtg tgcttcgccg cattttctac tgtccgtgcg
     4021 ggattgaagc tatgtgcacc ttgacaaccc tctaccagtg tgcgaccctt cactccactt
     4081 gcgagcgagt gagtgaggaa cgctttgagc gaggtttcgg agacggcgcg aaggaagagg
     4141 atctcggggt tgatgctcaa gatctcgcgc ctgctcgctc cgcccatcca cccgcgagta
     4201 tccacggtag ccgtaccgtc cgccctacga ctaattagcc tggcgggccg ggacggcgtc
     4261 ctccggtccg cctagagccg caagacggga acatcccgtt cttcggcaag gttggggcaa
     4321 gatctcttac ttggaatggg gcaaaccgtg gaaaacacag cattaaacat cgaatacaag
     4381 ccgcgtacgg gtagcgaagc catgtgcgag gcgctactcg acgaaggggt cgagtacctc
     4441 ttcgggtacc ctgggggcgc gatcatgcct ttctaccacg ccctccccga ctacccactg
     4501 aaacacatcc tcacccgcca cgaacaggcc gcggcccacg cggctgacgg ctacgccagg
     4561 gcgacaggcg gggtcggggt gtgcgtggcc acgagcggac ccggtgccac caacctggtt
     4621 acggggctgg ccacggccca gatggactcc gtgccgctcg tggcgataac gggccaggtc
     4681 ggccggccgg cgatgggcaa agactccttc caggagacga acgtgatggg catgacgctg
     4741 cccttcacca agcactcctt cctggtcgaa gaccccgacg aactctaccc ggcgatgcgg
     4801 cgggcattcg agatcgcccg ctccggtagg cctggccccg tcctggtaga cgtccccaaa
     4861 gacgtgcagc tcgcggccac gggcgacaac ggctaccggc ggctgcggga ggcgccgcct
     4921 cctgtggagt cgcccgggct ggagcgggca gcgatgcttt tgcggaacgc gaagcggccc
     4981 ttgatcctgg ccggacacgg cgtcacactc ggtagcgccg agaaggaact caggctgctc
     5041 gccgaaagga cgggcatccc agtcgtgacg acgctgctcg gcataagcgc cttccccgac
     5101 gaccacccgc tgtacctggg ctggccgggg atgcacgggc aggccagcgt caaccgtgcc
     5161 atagaccgct ccgacctcct tttcgcggtc gggatgcgct tcgacgacag gatcacgggc
     5221 gcggcccaca agttcgcccc acgcgcgaag atcatccaca tcgacgtgga cccctcggag
     5281 ctcggcaagg tcataaagcc gaccgtgggg atagccgcag acgcgaaggc ggcgatgcgt
     5341 tcgatactgg gacacctgga taatgccccc gactcaggcg agggatgcaa acagtggcgc
     5401 gaggaactgg cgacgagcca ggagccggag aggcgccgca aggagtcggc gcggggcgag
     5461 tacggtccga tacaggtaat ggaggccata aaatccgtgg cgggtcccga cctgcggctc
     5521 gtcaccgacg tggggcagca ccagatgtgg gcggcccagt tcttcgagtt caccaaggcc
     5581 aacagccaca ttacgagcgg gggcctgggc acgatgggct tcgcgctgcc ggcggcgatg
     5641 ggcgtggctt tcgcctatcc ggaagacccc gtatgggtca tagccggcga cggcggcatc
     5701 cagatgaaca tccaggagct cgccacgata agggacttcg ggctgaacat aaaggtcgcc
     5761 gttctgaaca acggttattt ggggatggtg cgccagtggc aacagttctt ccacaaccgc
     5821 cgctactccg agacgcccat aaccgggccc gactacgagc agctctccgc ctcctacggg
     5881 ctcgcagggc gcaccgtcag ggatggcgac gacgttgagg cagcggttcg atgggctgag
     5941 gagcaggagg gctgcaccat actggacttc cacatcgacc ccgaggcgaa cgtctacccc
     6001 atgatcccat cggggatgtc cgtcaacgag atgatcgagg aagacggtgc ctgagtcggc
     6061 aacgggcgtc gccctgaaca ccatcgaggt ccgcttcacg ggcggcatgc tcgccctgaa
     6121 caggctgctc atgacgctcc aaaacaagag gatgcccgtc gcgggcttca cgctgggcgg
     6181 cgacagcgac gggatgcggg ccaccatcct gctcgactgc ccgcccgagc cggcgctgcg
     6241 ctacacggcc ctcatctcgg ccctcgaaga cgtaagcgaa gcgggaccgg ccaagactat
     6301 agaggtggcc ctggtcgaga cctcgcaaga ctggcgcgag cccgcggagc gatcgggcgt
     6361 cgaagcgcac gaggacggtg gcacggtctt ggcatcgggc gagcccgaga aggtggaggc
     6421 cttcctcgcc gctcttggcg gcggcgtcga agacgtggtt cggctgggtc ctgtggcccg
     6481 tccggatgcg gggggaggag tctagatggc acgggtatac cgcgagggag acatagggaa
     6541 cgaggtcacg agtaggagag tcgccatcct gggctacggc agccagggcc acgcccacgc
     6601 cctgaacctg aaggagtccg gctgcgacgt gagggtcggg ctctacgaag gctcgaagag
     6661 ctggccgaaa gcggaaaggg acggcctgca ggtgcagacg atcgctgatg ccgtaagggg
     6721 ggcggacgtg gtgatgatgc tgctccccga cgagaagcag cccgccgtct tcgaggccga
     6781 ggtcgggccg agcctctcag agggcgatct catgctcttc gcccacggct tcaacatcca
     6841 cttcaaccag atcgtcgtcc cccccgaggt cgacctgggt ctcgtcgccc ccaagggacc
     6901 cggtcacgtg ctgaggcgtc tttacgtcga gggcaagggg atgccttcgc tcttcgcggt
     6961 cgagaacgac gcttcggggc aggctcggga tgtgatcctc tcctacgccc gcgccatagg
     7021 gagcggccgc gccggcatca tcgagaccac gttcgcggaa gagaccgaga cggacctctt
     7081 cggggagcag gcggtgttgt gcggaggact cacggcccta cttacggccg gtttcgagac
     7141 gctcgtcgag gcgggctacc agcccgagct cgcctattat gagtgcgtca acgagctgaa
     7201 gctcatagtc gacctgatct acgagggcgg gcttgcgggg atgcgctact cgatctccaa
     7261 cactgcagag tacggcgact acacggcggg accgaagatt atcgacgaag ccgccaaaga
     7321 gaggatgcgt tccatcctcg cggacatcca gagcggcaag tgggccaaag actgggtgct
     7381 cgagaaccag gcgggcggat ccagcttcct cgcgatgcgc cgcagggagg ccgggtcgca
     7441 ggtggagaaa gtcggacagg agctgcgggc gctcgcggcc gagggtatag agacgcccgc
     7501 aggctccgct ggaggtgaga acctatgagt cgtaaagctt tcgcggcgtc ggacgccgag
     7561 tgggcctacc acggcggcga gctcgtgagg gtcggcgaca ttcgcctctc acccgcgacc
     7621 cacgccctga actacggcac gggcgtcttc gagggcataa gggcctactg gagcgagcgg
     7681 cgcgggacgt tgcaggtcct gaagatgcgc gaacactacg agcgtttcga gaggtcctgc
     7741 cggctgctac gcatagagtt gcccaatacc gtcgacgagc tctgcgacgt cacgctggag
     7801 atcctgcggc gcaacgcccc gcgccaggac acctacgtca ggcccctcgc ttacaaggcc
     7861 gccgagtccg tgggggtgaa cctgaagggt gagaccgaac tctctatctt cacggttcct
     7921 atgggaaact acgtcgagct tacaggcctg agagtctgtg tctcttcctg gcgacgcacg
     7981 ccggataatg ccatcccggc ccgcgccaag tgcacgggtt cctacgtcaa cacggcgctc
     8041 gcggtcgacg aggcgcacat gtcgggctac gacgacgcca tcttccttac ccaggacggc
     8101 caggtctccg aggcgagcgc ggcgaacatc ttcctggtca ggaaaggccg gctcatcacc
     8161 ccgcccgtaa cggcggacat cctggagggt attacgcgcg acgccgtcat ggagctggcg
     8221 agcaaagaac tcgggatgcc ggtcatagag cgtgcggtgg accgcacgga gctctacgcc
     8281 gcggacgagg tcttcctctc ggggacgggg ttccagatag cgcccgtggt cgaggtggac
     8341 gaccgtcccg tcggcaccgg cagcataggg cccgtcgccg agcgcctgca ggaactgtac
     8401 ttcaaggccg cgcgcggcga gtgggaggag tacgccgatt ggacagtcgc cgtcgaggtt
     8461 gcggagcggg cggcgggatg agatactgcc gcccttcatg aaggagaaca tctagacatc
     8521 ttgcgcgttt ctcgccacag cctacgacta accagggtgg ccgccgggcg aattaccagc
     8581 cccggtatct agccggctgt ccgcggccac cagccccgag tcgcatctct tcgacagatt
     8641 tccaaacgta ggaggacgag acatatgcgt cgagtagaga tcttcgatac taccctccgt
     8701 gatggcgagc agtcgcctgg catctctttg tccgtagagg agaaggtaga gatagcccgc
     8761 cagctcgccc gcctgaaggt ggacgtaatc gaggcagggt tcccgatcac ctccgaggga
     8821 gacttcgagt cggtgagccg gatcgcctcg gaggtcaaag gccccaggat cgcgggcctc
     8881 gcccgcatcc acgagcagga catcgtgcgc gcctgggagg ctgtgcagtg gtcacagagg
     8941 ccacgcatcc ataccttcgt gggcaccagc gacctgcaca tagagcatca gatgcgctcc
     9001 aaccaggaag acatcctgcg gagggccggc gaggccgtcg tcttcgcgaa gagcctctgc
     9061 gagaacgtgg agttctcccc gatggacgcg acgcgcacgg acataggcta cctctccgag
     9121 atcgtcgcgg cggccgtcga ggcgggggcc gacgtggtca acatcgccga cacggtcggc
     9181 tacaccacgc cggttgaatt cgcggacttc ctgaaagagc tacaagagcg ggtgcccgcg
     9241 ctccaggagc gcgtcctctc ggtccactgc cacgatgacc tcgggatggc cgtcgccaat
     9301 tcgctcgcgg gggtcgaggt cggggccggg caggtcgagg tggccgtcaa cggtatcggg
     9361 gagcgggcgg ggaatgcctc gctggaggag gtcgtgatgg cgctggccac gcgctcggac
     9421 ttctacgggg caagggtggg cgtcgagacc cgccagctcg ccaacacgag ccgcatggtc
     9481 tccaacatca cgggctacga ggttcccccg aacaaggcca tcgtggggcg caacgccttt
     9541 ctgcacgagt cgggcatcca ccaggacggc gtgctcaagg acaggcggac gttcgagatc
     9601 atgaccaagg aagacatagg cctggaaggg acgaacatct tcctcggcaa gcactcgggg
     9661 cgccacgccc tcaaggacgc cctggaagag ctcggctact acgtagaggg cgatgtgctc
     9721 aagcgggcct tcacgcgctt caaggagata gcggaccaca agaagaccgt aactgcagca
     9781 gatctcgagg ctatagccgc agacgaggtc ggctccttcg agggcaagtt cgtcctggag
     9841 tctttcaggg tcgtcgccgc cacaggccgc cagagcaggg cggccgtcac catctcccac
     9901 gccgaacagg gcacgttcga ggccgaggcc gagggcgagg gaccggtcga cgcggtcttc
     9961 cgggccatcg acgccgcgac gaacataaag ggccgtctga cggacttccg catcgacgcc
    10021 gtgacgggcg gcaaggacgc cctgggcgag gtcagggtct ccgtggagtt cgagggggga
    10081 gattatgccg gacgcggcct ctcccaggac gtcgtcgagg cggctgcgcg cgcctacgtg
    10141 cgggccgtca acgtgtatac ggccggcatg gtcggtgcca ggtgggacgc gcaggtagcg
    10201 ccgtagcaac ccggggaaca cggcaagccg cgttaccggg ttacgaacgc ctggacgcgc
    10261 aggtgcccgt cctctacgcc ccacggctgg ccgggaaggc cagagagcta tccggctatc
    10321 tcacgcaggg ctacagggcg ctcggacgga tactcaccgt cgagcctccg gggatcgtcg
    10381 catacctggt ggcagacgaa gcctgggcag atgcgccgcg cgataacgcg cgcccttatc
    10441 cgcctggtct cccctacttc acccgttccg tcgagcctcc cgtcctcgtg ctcccggaag
    10501 agctatccac cgtgttcagg ccgcgtaccg cggcgacgct accccttacc gtctggcacg
    10561 agctggcaca cgctttcctg ctcgggcggg aggtcg
//
LOCUS       DATMVF010000226         9386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_492412, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000226 DATMVF010000000
VERSION     DATMVF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492412"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VN178_11730"
     CDS             <1..558
                     /locus_tag="VN178_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWS81683.1"
                     /translation="GFGDPEASVVAFGLAPAAHGANRTGRFFTGDRSGDFLFSSLHRT
                     GFANKPVSRNKDDGLRLSGLWISAAVRCAPPRNRPTPEERDACLPYAARELEALDAKV
                     IVCLGAFAWDAALRLLGKRPKPKFAHGAECEAGSLVLIGCYHPSQQNTFTGVLTPGML
                     DAVLSRARSRSLELSGAREDEGLVR"
     gene            570..1076
                     /locus_tag="VN178_11735"
     CDS             570..1076
                     /locus_tag="VN178_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81684.1"
                     /translation="MDFFETVRVRAIVPVAILLLAVLLLAWACSGETRKPDERSTPTR
                     ENTEVTKEPTSASTTRLETTEDSPPEITLGDPDGKPDVIIRLEGDPGTTFSGLCSVGE
                     ERSVLSGRVPKRFAYQLKGRQLSCRIEKRDGGTGDLEVILVAGDSTRSVQQTNAPGGV
                     IKVSYEDS"
     gene            1254..1817
                     /locus_tag="VN178_11740"
     CDS             1254..1817
                     /locus_tag="VN178_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWS81685.1"
                     /translation="MNREQSWELMCTWTESDSLRKHMLAVEAAMRAYARRFGEEEEKW
                     GITGLLHDMDYEKHPTPDEHPMVGVRELESRGYPEDVIHAIKGHADYLDVPRDTLMSK
                     TLYAVDELSGFIVACALMRPEGLENMKAKSVRKKMKQKSFAAAVNRDDIVRGAEELGV
                     DLNEHIEFVAHALKERSGELGFTPREA"
     gene            1898..2944
                     /locus_tag="VN178_11745"
     CDS             1898..2944
                     /locus_tag="VN178_11745"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HWS81686.1"
                     /translation="MMVVFGGRSGEHEVSLASARAITRALRKSGRYQVVPVGITREGR
                     WISSGEPMRQLESQSGYLPDGSDLDVSGPPASTTEKLPAGLASVDVVFPVLHGPYGED
                     GTIQGMLELAGVPYVGSGVLGSAAGMDKPTMKKIFAHHDLPQVRWVALTRGQWERDRE
                     TRIEEIETSIGYPCFVKPANLGSSVGIGKAVNAEELGEALDTASNFDRRIIVEEGVDA
                     REIEVSVLGNEDPDVSVPGEILIKKADFYDYEAKYVEGGMELVVPAPIRDEITQEITR
                     IAGTAYGAIDAAGMARVDFFLERGTDRLLLNEINTIPGFTPTSVYAELWAASGLPYEE
                     LLDRLIQLALQRHG"
     gene            2947..3960
                     /locus_tag="VN178_11750"
     CDS             2947..3960
                     /locus_tag="VN178_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWS81687.1"
                     /translation="MPFALLQETTANLADDATKVGKGVVEEPLSIFTELKEFLQGVYD
                     YIYSTQFVGKVLASVLVVVLGVLVFRILTRGVPRVLQWRRSSRESLLDEEAVARVKRQ
                     DTAITLIRNALRYVVFIIVVLVILSIFFNNPLPAVAGTTIIAAVIGFGAQSFLRDVIA
                     GFSIVFEGQYSVGDFVHIKPQDVSGIVEELGLRMTKIRSLSGEVSYVPNGAMQGVINY
                     VSGRQRFNVEVQVSDPEAATRVEHALGEASELYLSPPRLIERSEADGRVRMRIVAGVL
                     PSMAWLVEENLTQRIKAAAGEDSLAAEPLIYKVDQANLRRIRSLLPQEAERNLPRQAD
                     RKL"
     gene            complement(4075..4707)
                     /locus_tag="VN178_11755"
     CDS             complement(4075..4707)
                     /locus_tag="VN178_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWS81688.1"
                     /translation="MSDIPLFPLNVVLMPGAPLPLHIFEERYKQMVNECLEQEVEFGM
                     VFADESGTRQVGCTAKIVELVERYEDGRMLILVEGSRRFKLNNVLTGKPYYVGEIEYF
                     QDEPDEDIDTLAEECIALLERVVEAATEGSVGIEIEPPYRNLSFAIAGRVEFEPEIRQ
                     QILELTSEQQRLQKVKELLGAAAERLERERQTREKAQTNGYLRGDWQPGS"
     gene            4895..5686
                     /locus_tag="VN178_11760"
     CDS             4895..5686
                     /locus_tag="VN178_11760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWS81689.1"
                     /translation="MTQGGLHAGRVALVTGAGRGIGAATARLLAREGATVALAARTEE
                     EISSVAHEISSRGSTALPVSLDVADEGSVSACFERVQGELGNVTILINNAGTPGIPLP
                     VAATRPDAWRRAFDVNVTGAFLCAREALPSMVSANWGRIVNVSSAAARHPVAGMAAYG
                     ASKAALDQLTRVLALEGGPYGIAVTGVYPGVVDTRMQEETRGFGQELIGKQLHRMFQG
                     YRDFGMLRPPEEPAELISYLCTPAAWRLSGHIIRLEQLESLKEQP"
     gene            complement(5835..5987)
                     /locus_tag="VN178_11765"
     CDS             complement(5835..5987)
                     /locus_tag="VN178_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81690.1"
                     /translation="MMELALLLLQQGTTANPAPIGIFRFFVGFVLVVAVYTSFLGFTY
                     WISKDE"
     gene            complement(5984..7327)
                     /locus_tag="VN178_11770"
     CDS             complement(5984..7327)
                     /locus_tag="VN178_11770"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWS81691.1"
                     /translation="MPIGNLDVPIIGKNVVIAVLVQTHILFATFIIGAVLIAATSEYL
                     GMVTKQNNYERFARTLARFVVLLFASGAALAITFVLALVTLFPVFFSILQNIFFWVFL
                     VEAFMFLGQIIIVYAWYNVWDKLAYRKSLHVVFGFIAGFFGLMAMTMIDAVASFMLTP
                     AEVTSEASLVARSFLNQTMVPLNMHRFVGNFSYAGFLIAGWAGWRYLRTTREDDREYY
                     DWMGHFGLLWGFGFLIMQPIIGYGYLKGIRESSPDAFNTIMLGSKAWVFNLLVLWIAV
                     LSIASTAYFVHKLRFAIRPMETLRKLSVGALGFTALFTVLNIIPADADLIPQIGLVFG
                     QGEGTQIPLGSMYPWKYIGLIGLMLVGFFIVALYLRASATGFHWGRASRWSQYALMAC
                     AFFVVLTMITMGYTRETARRVDNNPAYLINDCVTLDQTIVKEGCPATPITEGAGG"
     gene            complement(7341..7826)
                     /locus_tag="VN178_11775"
     CDS             complement(7341..7826)
                     /locus_tag="VN178_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochromesubunit B of the bc complex-like
                     protein"
                     /protein_id="HWS81692.1"
                     /translation="MYAPATGKAKPVEDEVITEENVFERPDETIAFFPGQVLKDGYVS
                     TFILLACVVLSYAAPAPLTEPANTATVEYVPRPEWFFFFYEQMLMFFPGYALISFGAV
                     VVPTIFIGLLFAVPWLDRSPQHYNSISRPFASVIGVLVVIIVVLNMLLAVSRIINFPT
                     G"
     gene            complement(7906..8736)
                     /locus_tag="VN178_11780"
     CDS             complement(7906..8736)
                     /locus_tag="VN178_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HWS81693.1"
                     /translation="MIGRIRTQTSEASQFLVDWMEHRTGIVTMLEHFLYEPVPKRGAW
                     LYTLGSAVLFTITLQFLTGILLLFYYAPTTDDAWNSVDYIMTEAYFGQLIRGIHYWSA
                     NFLMAFIGLHMGQVFFSGGYKAPRELNWIVGVILLLLVIGLSFTGYLLRWDQDGFWAS
                     VVGMKIGSYSPFVGGYVIHFLLGGDVVGPATLSRFFAIHVWLLPALIAPLIGAHLYLL
                     RRHGVFGSEFEYTDRLAKLHERQRLEEYARYETDEDEGEPYVGRRDRADREGYERDGE
                     "
     gene            complement(8753..>9386)
                     /locus_tag="VN178_11785"
     CDS             complement(8753..>9386)
                     /locus_tag="VN178_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWS81694.1"
                     /translation="LTIPPAAFVLSPVIKTDLLGESDVGSGWVEVASVSEVSAEEPSH
                     FKVEFPLEQVYGERAIQKKHPGSQKSDMNYKLDNAIWLSWKAPIEQQGRQGSTGDILG
                     KPERPAILDQKSEGFTESERKEIMSRLNVLSNSCAHLGCPVRWFTHEQLFLCPCHGGL
                     YDINGGWVGGPPPRGMYRYTEAEVRENGKLYVRHKYDIEPGLDKQQPYVV"
BASE COUNT         1879 a         2912 c         2912 g         1683 t
ORIGIN      
        1 ggcttcggag atcctgaggc cagcgtcgtc gccttcggtc tcgccccggc cgcccacggg
       61 gcgaacagga caggacgctt ctttactggc gaccgttccg gcgactttct cttctcatcg
      121 ctccatcgca cgggatttgc caacaagccg gtctcacgga acaaggacga cgggctgcgt
      181 ctttcgggac tctggatctc ggccgccgtc cggtgcgccc cgccccggaa cagaccgacc
      241 ccggaagagc gcgacgcctg cctcccttac gccgcccgcg aactggaggc gctcgacgcc
      301 aaggtcatcg tctgcctcgg cgcgttcgcc tgggacgccg ccttgcgact gctagggaag
      361 aggccgaagc cgaagttcgc gcatggcgcg gagtgtgagg ccggctcgct cgttcttatc
      421 ggttgctacc acccgtcgca gcagaacacc ttcaccgggg tgctcacccc cgggatgctc
      481 gacgccgtcc tgtcgagggc gagatcccgc tccctggagt tgtccggagc gagagaagac
      541 gagggtctcg ttagatagaa tggatgctcg tggatttctt tgagaccgta agagttcgtg
      601 cgatcgtgcc ggtggcaata ttgctcctcg ctgtgctgct cttggcctgg gcctgctccg
      661 gtgagaccag aaagcccgat gagagaagca cccctactag ggagaatacg gaggtcacga
      721 aggagccgac gtccgcttca accaccagac tggagacaac agaggattcg cctcccgaga
      781 tcaccctcgg cgaccccgac ggtaagcctg acgtcatcat ccggctggag ggggaccccg
      841 ggaccacgtt ctccggcctc tgctcggtgg gagaggagcg tagcgtgctg agcggccggg
      901 tgcccaagcg cttcgcgtac caattgaagg gaaggcagct ctcctgccgc atcgagaaac
      961 gggacggggg caccggcgat ctcgaggtca tcctggtcgc cggcgattcc accagatccg
     1021 tccagcagac gaacgcgccg ggcggcgtca taaaagtctc gtatgaagat agttaggcat
     1081 caggtttcag gctacaggca tcagggaatg ttttgggccg cctcgtttcg aagtggacgg
     1141 tatacagtag caaagcgaga cagaatctca gacgcagcag gaagtcccgc tggcaccaac
     1201 ctgagacctg acgcctgaaa gctgtagcct gtcgaccgaa gggagacaat taattgaacc
     1261 gcgaacagtc atgggagctc atgtgcacgt ggacagagag tgactccctg cgcaagcaca
     1321 tgctcgccgt cgaggccgcc atgcgggcct acgcccgccg tttcggcgag gaggaggaga
     1381 agtggggcat aaccggcctc ctgcacgata tggattacga gaaacacccc acgccggatg
     1441 agcatcctat ggtcggtgtc agggagctgg agagcagggg ctatcccgaa gacgtcatcc
     1501 acgccataaa ggggcacgcc gactacctgg acgtaccccg cgacaccctc atgtccaaaa
     1561 cgctctacgc cgtagatgag ctctccggct ttatcgtggc ctgcgccctc atgcgtcccg
     1621 aaggtctcga aaacatgaag gcaaagagcg tccgcaagaa gatgaagcag aagtccttcg
     1681 ctgcggccgt gaacagggac gacatcgtaa ggggcgccga ggaactcggc gtggatctca
     1741 atgaacacat agagttcgtg gcccatgcgc tcaaggagcg gtcgggcgag ctcggtttca
     1801 cgccgcgaga ggcctgaaga gtacgtcagg cgtacgcctg gcaagccctt tgctaggata
     1861 ggcgggctgg agaagagagg agcggcgttt ggtcagggtg atggtcgtct tcggaggccg
     1921 cagcggagag cacgaggtgt ccctggcctc agcgcgcgcc ataacgcggg cgctgaggaa
     1981 gagcggccgc taccaagtcg tacccgtcgg cataaccagg gaggggcgtt ggatctcctc
     2041 tggagagcct atgcgccagc tggagtcgca gagcggctac ctgcccgacg gctccgacct
     2101 cgacgtctcg ggcccgccgg cctccacaac tgagaaacta ccggcgggcc tcgcttccgt
     2161 ggacgtggtc ttccccgtcc tgcacggtcc gtacggggag gacggcacca tacagggcat
     2221 gctcgagctc gctggtgtcc cctacgtcgg cagcggggtg ctagggagcg ccgccggcat
     2281 ggacaagccc acgatgaaga agatcttcgc ccaccacgat ctcccgcagg tcaggtgggt
     2341 cgccctgacc agagggcagt gggagagaga ccgggagacc cggatagagg agatcgaaac
     2401 ctccattgga tacccctgct tcgtgaagcc tgcgaacctc ggctcgagcg tcggcatcgg
     2461 caaggccgtg aacgctgaag agttgggtga ggccctcgac accgcttcga acttcgaccg
     2521 gcgcatcatc gtcgaggagg gtgtggatgc ccgcgagatc gaggtctccg tgctcggcaa
     2581 cgaagaccct gacgtctccg tcccaggtga gatcctaatc aagaaagcgg acttctacga
     2641 ttacgaggcg aagtacgtcg agggcgggat ggagctcgtc gtccctgctc cgatccggga
     2701 cgagatcaca caggagataa cgcgcatcgc cggcacagcc tacggggcca tagacgcagc
     2761 gggcatggcc cgcgtcgact tctttctcga acgcggcacg gaccggttgc tactcaacga
     2821 gatcaacacc atccccggct tcacccctac gagcgtctac gcggaactct gggccgcgag
     2881 cgggctccct tacgaagaac tgctcgaccg cctcatacaa ttggccctcc agcgccacgg
     2941 ctaggtatgc ccttcgccct gctccaggaa acgacggcca acctcgccga tgatgccacg
     3001 aaggtcggca aaggggtcgt ggaagaaccc ctatccatct tcacggagct aaaggagttt
     3061 ctgcagggcg tctacgacta tatctatagt actcagttcg taggcaaagt actcgcttct
     3121 gtgctagtcg tcgtcctcgg ggtccttgtc ttccgcattc tgacccgtgg cgtgccacgg
     3181 gttctgcagt ggcggcggag cagcagggag tcgctccttg acgaggaggc cgtggcccgc
     3241 gtcaagcgcc aggacaccgc gatcaccttg atacgcaacg cgctccgtta cgtcgtcttt
     3301 atcatcgtgg tactcgtgat cctgagcatc ttcttcaata acccgttgcc cgccgtggcg
     3361 ggaaccacaa tcatcgccgc cgttataggt ttcggggctc agagcttctt gcgcgacgtg
     3421 atagccggtt tctcgatcgt cttcgagggg cagtacagcg tcggggactt cgtccacata
     3481 aagccgcagg acgtctccgg gatcgtcgag gagctgggac tcaggatgac caagatccgt
     3541 tccctctcgg gtgaggtgag ttacgtcccc aacggcgcca tgcaaggcgt tatcaactac
     3601 gtctcgggcc ggcagcggtt caatgtggaa gtgcaggtct cggacccgga ggcggccacg
     3661 agggtggagc acgcactagg cgaggcctcg gaactgtacc tgagtcctcc ccgcctcatc
     3721 gagcgaagtg aggccgacgg ccgcgtcagg atgcgaatcg tcgccggggt cctgccctcg
     3781 atggcctggc tcgtcgagga gaacctgacg cagcgcataa aggccgcagc cggggaagac
     3841 tccctggccg cggaacccct gatctacaag gtggaccagg cgaacttgcg caggataaga
     3901 tccctcctcc cccaggaagc ggagcgaaac ctcccccgcc aggcggatag aaagctttag
     3961 ggcagtcagc gataagcttt cagccttcag cagatgcccg ccggtcgtta ccctggcgcg
     4021 tccgatcctc tctacaggat cagtagctga tagctgaccg ccgaaagctg acggctaact
     4081 ccctggctgc cagtcgccac gcaggtaccc gttggtctgg gccttctcgc gcgtctgccg
     4141 ctcgcgctcc agtctctcgg ccgccgcgcc cagaagttcc ttgaccttct gcaggcgctg
     4201 ttgctcggag gtgagctcca ggatttgctg gcggatctcc ggctcgaact ccacccggcc
     4261 cgcgatggcg aatgagaggt tcctgtaagg tggctctatc tcgatcccga cggaaccttc
     4321 ggtcgcggcc tcgactaccc gctcgagcaa tgctatacac tcctcggcca gcgtgtcgat
     4381 gtcttcgtcg ggctcgtcct ggaagtactc gatctcaccc acgtagtaag gtttgcccgt
     4441 gaggacgttg tttagcttga agcggcgcga accttcgacc aggatcaaca tccgaccgtc
     4501 ctcgtagcgc tcgactagct ccacgatctt cgccgtgcaa cctacctgcc gcgtccctga
     4561 ctcatcggcg aagaccatcc cgaactcgac ctcctgctcc aggcactcgt tcaccatctg
     4621 cttgtagcgc tcctcgaaga tgtgcagcgg cagaggcgcc ccgggcatca gcaccacgtt
     4681 gagcggaaag aggggtatgt cggacaagcg tacgctcctt tgctctttga ccgaattcta
     4741 tctcacctgg caccggccat gctcgacacc ccacagcgac ggcgcctgct gccgatcggt
     4801 gcctagggta agctagtggg agagaagata gggtcagagt gtggaaggcg ggatccatgg
     4861 ccgtaagaac gtacgtctcc gccgaaaaca ggggatgaca caaggcgggt tacacgccgg
     4921 tcgcgtcgcg ctcgtaaccg gcgcgggacg cggcatcggg gcggccacgg cccgacttct
     4981 cgcccgcgag ggcgcaaccg tcgccctcgc cgcccgcacg gaggaggaga tctcctccgt
     5041 ggcgcacgag atctcctcgc gcggctcgac agccctaccc gtgagcctgg acgtggccga
     5101 cgagggctcg gtctcggcct gtttcgagag ggtgcagggc gaactcggca acgtcaccat
     5161 cctcataaac aacgcgggca caccaggcat cccgctcccc gtcgcggcca cccggccgga
     5221 tgcctggcga cgcgccttcg acgtcaacgt gaccggggcc ttcctgtgcg ccagagaggc
     5281 gctccctagc atggtctcgg cgaactgggg acgaatcgtg aacgtctcca gcgccgccgc
     5341 caggcatccg gtcgcgggga tggccgccta cggcgcctcg aaagcggccc tcgaccagct
     5401 cacccgcgtt ctggccctgg agggtggacc ttacggcatc gccgtaacgg gcgtctatcc
     5461 gggcgtcgtg gacaccagaa tgcaggagga gacgcgaggc ttcggccagg agctcatcgg
     5521 caaacagtta caccgcatgt tccagggcta cagggatttc gggatgctcc gtcctcccga
     5581 ggagccggca gagctcataa gctacctctg cactcccgcc gcctggcgcc tcagcggcca
     5641 catcatccgc ctcgaacagc tcgaatctct caaggagcag ccttgactct ggccggcaag
     5701 acggcggtcg taaaagggcc tcgagcggca tcgagcaggc cgcgtacgaa gccctttcag
     5761 gatcctcgca agaagcgagg gccgcagcgt tcgccgcagc cctacctgaa acctgtaacc
     5821 taataccttc ttctctactc gtccttgctg atccagtacg tgaatcccag gaaggaagtg
     5881 tagaccgcga ccacgagaac gaagccgacg aaaaaccgga agatcccgat gggcgccggg
     5941 ttggccgtgg ttccttgctg gaggaggaga agcgccagct ccatcatccc cccgcacctt
     6001 ccgtaatggg cgttgccgga cagccttcct tgacgatggt ctggtccagc gtcacgcagt
     6061 cgttgatgag gtaggctggg ttgttgtcga cgcggcgcgc ggtctcgcgc gtgtaaccca
     6121 tcgttatcat ggtcagaacg acgaagaagg cgcacgccat cagcgcgtac tggctccacc
     6181 ggctcgccct tccccaatgg aaccctgttg cagaagccct caggtacagg gcaacgatga
     6241 agaagccgac gagcatcagc ccgatcaggc cgatgtactt ccaggggtac atgctgccca
     6301 gcggaatctg tgtcccttca ccctgcccga agaccaaccc tatctggggt atcaggtcag
     6361 cgtcggccgg gattatgttg agcacggtga acaacgcggt gaagccgagg gcgcccacgg
     6421 agagcttacg caacgtctcc atgggtctta tggcgaagcg caacttgtgg acgaagtaag
     6481 ccgtcgaggc gatgctgagg acggcgatcc agaggacgag caggttgaag acccacgcct
     6541 tgctgccgag catgatggtg ttgaacgcgt cgggcgagct ctcgcggatg cctttgaggt
     6601 aaccgtagcc gatgatcggc tgcatgatca agaacccgaa gccccagagg aggccgaagt
     6661 gtcccatcca gtcgtagtac tccctgtcgt cctcccgggt ggtcctcagg tacctccagc
     6721 cggcccagcc ggcgatcaag aacccggcgt aggagaagtt ccctacgaag cggtgcatgt
     6781 tgaggggtac catcgtctgg ttgaggaagc tgcgggccac gaggctagcc tcggaggtaa
     6841 cctcggcggg cgtgagcata aaggaggcga ccgcgtcgat catggtcatc gccataagcc
     6901 cgaagaagcc tgcgataaaa ccgaagacga cgtgcagact cttgcggtag gcgagcttgt
     6961 cccacacgtt gtaccaggcg tagacgatga tgatctggcc caagaacatg aacgcttcca
     7021 ccaggaacac ccagaaaaag atattctgca ggatggagaa aaagacgggg aaaagcgtaa
     7081 ccagggcgag cacgaacgtg atcgccaggg ccgcacccga agcgaaaagc agaaccacaa
     7141 agcgcgccag ggtcctcgcg aagcgctcgt agttgttttg cttggtcacc atcccgaggt
     7201 actcgctcgt agccgctatc aggacggccc cgatgatgaa ggtcgcaaag aggatgtgcg
     7261 tctggaccag aacggcgata acgacgttct tgccgattat cggtacgtca aggttgccaa
     7321 taggcaccgc tcggcctcct ctagcccgtg gggaagttta tgatccggct tacggccaga
     7381 agcatgttga ggacgacgat tatgaccact aggacaccta tgaccgaagc gaagggcctg
     7441 ctgatagagt tgtagtgctg cgggcttcgg tcgagccacg ggacggcgaa gaggagcccg
     7501 atgaagatcg tcggcaccac gacggccccg aaggatatca gcgcgtatcc ggggaagaac
     7561 atgagcatct gctcgtagaa gaagaaaaac cactcgggcc tcggcacgta ctccacggtc
     7621 gccgtgttcg ccggctcggt gagcggcgcg ggtgcggcgt aggaaagcac gacgcaggcg
     7681 agcagtatga aggtgctgac gtagccgtcc ttgagcacct gcccggggaa gaacgctatg
     7741 gtctcgtcag gtcgctcgaa gacgttctct tcggtaatta cctcatcttc aaccggtttg
     7801 gccttgcctg tcgccggagc gtacaccttc tacacctctt ctctctttct caagttccgt
     7861 accacaatcg taacagtggt acgccagcct tctcggtaaa agctgttact caccgtctct
     7921 ttcgtagcct tccctgtccg cgcggtcgcg gcggccgacg tagggctccc cttcgtcctc
     7981 gtccgtctcg tagcgggcgt actcttcgag gcgctgtcgt tcgtgcagct tggccagcct
     8041 gtcggtgtac tcgaactcgc tgccgaacac cccgtgacgc ctgagcagat agaggtgcgc
     8101 accgatcagt ggggctatca gggcaggaag cagccagacg tggatggcga aaaagcggga
     8161 gagcgtcgcg ggccccacaa cgtccccgcc aaggaggaag tggatcacgt acccgccaac
     8221 gaaaggcgag tacgaaccga tcttcatccc gaccacagag gcccagaaac cgtcctggtc
     8281 ccacctgagg aggtacccgg tgaaggaaag cccgatcacg aggaggagca ggataacgcc
     8341 tactatccag ttgagctccc ggggcgcctt atagccaccc gagaagaaca cctggcccat
     8401 gtgcaacccg ataaacgcca tcaggaagtt cgccgaccag tagtggatgc cgcggatgag
     8461 ctgtccgaag tacgcctcgg tcataatgta gtcgacggag ttccacgcgt cgtccgtggt
     8521 cggcgcgtag tagaacagga gcaggatgcc cgtcaaaaac tgcaacgtta tggtgaacag
     8581 caccgcgctg cccaaagtgt agagccaggc gccgcgtttg gggacgggct cgtagagaaa
     8641 gtgctcgagc atggtgacta tcccggtccg gtgctccatc cagtcgacca ggaactggct
     8701 cgcctccgac gtctgggtcc ttatcctgcc gatcatgctc ggtgctccct ctctagacca
     8761 cgtagggttg ttgcttgtcc aggcctggct cgatgtcgta cttgtgcctg acgtagagct
     8821 tgccgttctc cctgacctcg gcttcggtgt aacggtacat cccgcgcggc ggtgggcccc
     8881 cgacccaccc gccattgatg tcgtagagtc cgccgtggca cgggcaaagg aagagttgct
     8941 cgtgggtgaa ccaccgcacg ggacatccga ggtgcgcgca ggagttgctg aggacgttga
     9001 gccggctcat gatctctttg cgctcggatt ccgtgaaacc ctcgctcttc tggtccagaa
     9061 tcgccgggcg ctccggtttc cccaaaatgt cgccggtcga cccctgcctg ccctgctgct
     9121 cgataggggc cttccacgag agccagatgg cgttatccag cttgtagttc atgtccgact
     9181 tctgggagcc aggatgcttt ttctgtatgg ccctctcccc gtagacctgt tctaggggga
     9241 actcgacctt gaagtgcgaa ggctcctccg ccgagacctc agagaccgaa gcgacttcca
     9301 cccagccgga tcccacatca gattcgccca acaagtcggt cttgatgacc gggctgagga
     9361 cgaacgcggc gggagggatg gtcagt
//
LOCUS       DATMVF010000227         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_130700, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000227 DATMVF010000000
VERSION     DATMVF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130700"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1933
                     /locus_tag="VN178_11790"
     CDS             <1..1933
                     /locus_tag="VN178_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81695.1"
                     /translation="PGAGGISVIREDPQTARPGRACANHTALLMGLMLLLLCAMGFAR
                     GSQMGETEVLEKASFVPTLEEYYAKPTVETTAYYDRGKDLWWVVLTEQTSGKEVARFK
                     VADDSGGVSGVEISPGADGIEYPRLSEEKAIKRAAASPEVREEFTRHGPHSAEAKYEE
                     GAWTVRFYVDERGSIGGRPTEKGKEIATVGVDDKTWVLDYVYTGDQVGWNLARGVRGA
                     YGKQANYWWVWLPLALAFAAAFWHTDKLFAIRNLDIVALLGFLVSHGFFREGVVLEAV
                     VLWYPPLVYLLIRTLLMGFGIGEKVEKTSNLPLWLLMVLAGLAAGLVLGLNVDSRVID
                     VGYAGVVGADSILEGTSPYGTMPRDVGTGDTYGPLNYLLYVPFVLMFGFSGEWDFLPA
                     AHALTLFSFVAGAMALFITGYRLSGKDGAAALVFAWAAFPYTVYATNNNTNDIIVAAV
                     SAIGLAAAASPIARGASIAAGFAIKLYPLVLGPLWIMYEGRKRKPIIDFVLGGAGLLL
                     VTFWVILLDGHPIEAARLFYENTLAFQGDRVSPWSLFSQVPELAFLQRPLMAFAIFLS
                     ILVAFVPREKTLRRLAALSAALVIAFQLTVNYWFYAYITWFEPFVFVSLLLATNEKTA
                     LDSSQPSAFSGRPEEDGAT"
     gene            complement(1892..2065)
                     /locus_tag="VN178_11795"
     CDS             complement(1892..2065)
                     /locus_tag="VN178_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81696.1"
                     /translation="MRQGHLVGDSIERPQIFGLLGEHAPEQPVGDEAGRSAFSLALSI
                     SGCAILFRPTAEG"
BASE COUNT          471 a          843 c          871 g          512 t
ORIGIN      
        1 gccaggagct ggaggaatct ccgtaatccg ggaagatcca cagacagcgc gtccgggccg
       61 ggcgtgtgcg aaccacacgg cactactgat ggggctcatg ttgttgctgc tgtgcgcaat
      121 gggcttcgcg cggggttccc agatgggcga gactgaggtg ctggagaagg ccagctttgt
      181 tccgacgctg gaggagtatt acgccaaacc gaccgtcgag acgacggcgt actacgacag
      241 aggcaaggac ctctggtggg tcgtacttac ggagcagact tcggggaagg aggtcgcccg
      301 cttcaaggtc gccgacgatt ccggaggggt gagcggggtc gagatctcgc ctggagctga
      361 tgggatcgaa taccccagac tctcggaaga gaaggccatc aagcgcgcag cggcgagccc
      421 cgaggtcagg gaggagttca cgaggcacgg gccacacagc gcggaagcca agtacgagga
      481 gggcgcgtgg acggtccgct tctacgtcga cgagaggggg tccatcggag gcaggccgac
      541 ggagaagggc aaagagattg ccaccgttgg ggtggacgac aagacctggg tcctcgacta
      601 cgtttacact ggggatcagg tcgggtggaa cctggccagg ggcgtgcgcg gcgcctacgg
      661 caagcaggcc aactactggt gggtctggct gccgctggcg ctagccttcg cagctgcttt
      721 ctggcacacg gacaagctgt tcgccatacg caacctggac atcgtggcgc tgctcgggtt
      781 cctcgtctcg cacggcttct tcagggaggg ggtagtcctc gaggccgtcg tcctgtggta
      841 cccgccgctc gtctacctcc taatcaggac ccttctcatg ggtttcggga taggcgagaa
      901 ggtcgagaag acgagcaacc tcccgctgtg gctcttgatg gtgctcgccg gtctcgccgc
      961 cggcctcgtc ctcggcctca acgtcgactc ccgcgtcata gacgtgggct acgccggggt
     1021 cgtcggcgcc gacagtatcc tggaaggcac atctccctac ggcaccatgc cccgagacgt
     1081 ggggacgggg gacacttacg gccctctgaa ctacctgctc tacgtgccct tcgtcctcat
     1141 gttcggcttc tccggagagt gggatttcct ccccgcggcc cacgccctca cgctcttctc
     1201 cttcgtcgcg ggggctatgg cgctgttcat cacgggctac aggctctcgg gcaaggacgg
     1261 ggccgccgcc ctcgtcttcg cctgggcggc cttcccctac accgtttatg cgaccaacaa
     1321 caacaccaac gacatcatcg tcgccgccgt ctcggcgata gggctcgcgg ctgcggcctc
     1381 gccgattgct cggggggcga gcatcgcggc cggcttcgcc atcaagctct atccgcttgt
     1441 gctcggcccc ttgtggatta tgtacgaagg tcgcaagcgt aagcccatca tagatttcgt
     1501 gctcggcggg gccgggctct tactggtcac gttctgggtc atcctgctcg acgggcatcc
     1561 tatcgaggcg gcgaggctct tctacgagaa caccctcgcc ttccagggcg acagggtcag
     1621 cccgtggtcc cttttcagcc aggtccctga gctcgcgttc ctgcaacgac cactcatggc
     1681 gttcgcgatc ttcctctcca tactcgttgc tttcgtgccg cgcgagaaga cgctgcgccg
     1741 gctcgcagcg ctctctgcgg ccctcgtcat cgccttccag ctcacggtca actactggtt
     1801 ctacgcctac atcacctggt tcgagccgtt cgtgttcgtc agcctcctgc tggccacgaa
     1861 tgaaaagacg gctttggata gcagtcagcc atcagccttc agcggtcggc ctgaagagga
     1921 tggcgcaacc tgagatggac aaagcgagcg agaacgcaga acgtccggcc tcgtcgccga
     1981 cgggttgctc cggagcatgc tcgccaagca acccgaagat ttggggcctc tctatggagt
     2041 cgccgacgag atggccctgt cgcacgagga gatgggtgtc ctggcacggg cctacaccta
     2101 cagggagtgt acggctgccg gcgcgagggg cacggcaagg atgcgggagg aaggtgcccg
     2161 gtgcgcgcaa agccggatgg gtcgaaggtg ctggcgctgt gcgatgcgag cgagtcgagc
     2221 accagggagt tcgtccagaa ggcaggcatc ccgcggcgca ctcaccaccg ccggcggcgg
     2281 cgatttcacc gtgccgtagg ctctggtccg acagggcata atcgtttgct tgtgaaccct
     2341 actatagggt agcggcctca gtgtacgagg gccggacctg caatgccccg gcccttacac
     2401 ttaccctcat tcaccggagt gcgtggatat tagagatccc tccgacgcct ggcctcgaag
     2461 tacgcactct cggtcgtggc gagcgccgca gaaagcagcg tgagtaggtc gcggtagttg
     2521 tccccatcgg tcatgtcctc agagcgcatg aggctttcgc gccgaggtat ccccctctcc
     2581 tcggcgtggc ctatccaccc agcatcgagg tataagcttt ctgcgatcct ccactttacg
     2641 gagtcctagg aaactcgagg gggctcgctt actcgatcga caagggcgct ccgacta
//
LOCUS       DATMVF010000228         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_310474, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000228 DATMVF010000000
VERSION     DATMVF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310474"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /gene="gltX"
                     /locus_tag="VN178_11800"
     CDS             complement(<1..894)
                     /gene="gltX"
                     /locus_tag="VN178_11800"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWS81697.1"
                     /translation="MENNAKIRVRYAPSPTGRLHVGGVRTALFNWLFARKNGGTFVLR
                     IEDTDLERSTEESVEQLERSLRWIGLAWDEGPGTGGPHPPYRQTERFDLYREAARKLI
                     DSGAAYYDFATPEELAELRERARAEKRQPIYTGGEYREMDPDEARERIEAGEPYTVRF
                     KTPREGQTVVQDMIRGPVTFENANLEDFVLMKSTNTPTYNFAAAVDDAEMRISHVIRG
                     DDHLSNTPRQILLYKALGRDLPAFAHVPQVLGPDKKKLSKRHGAASVEDFAEQGYLPE
                     ALFNYLALLGAGYAADEEIFSP"
     gene            complement(1010..1477)
                     /locus_tag="VN178_11805"
     CDS             complement(1010..1477)
                     /locus_tag="VN178_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81698.1"
                     /translation="MSIGDVAANFGKGLFAGAAGTAAMTVSSTLEMKLSGREASQTPA
                     QAAEAVLNVEPKDDDSEARFSNLVHWGYGTGWGGVRGLLASAGLSGPLATAAHLGLVW
                     GAEQVVLPTLDVSAPVFKYGGKAIATDLLHHVVYATATGLAYSYLDRSQSVPR"
     gene            complement(1517..3601)
                     /locus_tag="VN178_11810"
     CDS             complement(1517..3601)
                     /locus_tag="VN178_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWS81699.1"
                     /translation="MSTIVRAPGRAIGAPLDRIDGPQKVTGAAPYAYEHRVGYPLYLY
                     PLQSDIARGRVASIDASAAEALPGVVIVVTHENAPRLADTSDKELAILQSDEVAFRGQ
                     YVGAVVAETFEVARHAANLVEVSYAEESHDSKLRPEGDDLYTPDQLNAGFPTDTEEGD
                     FDDAFASATVKLDETYTTPMEHNNPMEPHTTVAVWDEGDLTLYESTQGVHTVRRAVAR
                     VFGLDRDRVRVVAPHVGGGFGSKGLPHANTVLTTLAAQLIEGRPVKFALTRQQMFSGV
                     GYRTPTVQRIRLGADGDGRLTAISHDVIEQTSKIKEFAEQTAYATRMMYATQTRRTTH
                     RLAALDVPVPSWMRAPGEAPGMYALEAAMDEMAARCNLDPIEFRIRNEPEFDADTGLP
                     YSSRNLVACLREGAERFGWEGRDPAPRARMDGRWLVGTGVAASMYPVTRMPGTKARIR
                     SLDEGRYAVEIGAVDIGTGTWTALTQISADALGVGVEKIGLRIGDTGLPSASVAGGSS
                     GITAWGSTIFEAARVFRERYGEDPSEGDTVEAGTPENPETENYSMHAFGAHFVEARVN
                     EDTGEVRVPRMTGVFAVGRVINVKTARSQFIGGMTMGLGMALHEESLVDERFGHVVNH
                     DFAEYHIPTNADVGEIDVSWIDEKDPHVNPMGSKGIGEIGIVGSPAAVANAVYHATGV
                     RVLPITPDKLVR"
     gene            complement(3598..4584)
                     /locus_tag="VN178_11815"
     CDS             complement(3598..4584)
                     /locus_tag="VN178_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWS81700.1"
                     /translation="MKPYSYVRARDVGGAVAALAEAPDAAFLAGGTNLVDLMKLEVMT
                     PDVLVDVRRLTSESIEESPDGGVRIGAAITNSDLAADRTIRSRYPVLSQALLSGASGQ
                     LRNLATTGGNLLQRTRCVYFYDTTTPCNKREPGSGCSAIEGHNKNHAILGASEHCVAT
                     HPSDMAVAMAALDAFVLVRGHDGERRIPIEELHRLPGDEPQRDTTLEHGELITAADLP
                     PLPFAFNSKYRKVRERASYAFALVSVAAVLDVEEGTVRDVRLALGGVAHKPWRATKAE
                     QALRDAPANEANFRAAADIELEDAEPLRENAFKVLLARNIITRTLLELSEER"
BASE COUNT          688 a         1556 c         1565 g          887 t
ORIGIN      
        1 gggggagaag atctcctcgt ccgccgcgta cccggcgccc aggagcgcca agtagttgaa
       61 gagcgcttct ggcaggtaac cctgctccgc gaagtcctcg acgctcgcag cgccgtggcg
      121 cttggacagc ttctttttgt ccgggccgag gacctggggg acatgggcga aggccgggag
      181 atcgcgtccg agggctttgt acaggaggat ctggcgcggc gtgttcgaga ggtggtcgtc
      241 gccccttatc acgtggctga ttctcatctc tgcgtcgtcc accgccgcgg cgaagttgta
      301 ggtcggggtg ttcgtggact tcatcaagac gaagtcctcc aggttggcgt tctcgaacgt
      361 aacgggcccg cggatcatgt cctgcaccac tgtctggccc tcgcgagggg tcttgaaacg
      421 cacggtgtac ggctcccccg cctctattct ctctctcgcc tcatcagggt ccatctcgcg
      481 gtactcaccg ccggtgtaga tcggctggcg cttctcggcc cgcgcgcgtt cccggagctc
      541 ggccagttcc tccggggtgg cgaagtcgta gtacgccgcc cccgagtcga tgagcttccg
      601 cgcggcctcg cggtacaggt cgaagcgctc ggtctggcgg tacgggggat gggggccgcc
      661 ggtccctggg ccctcgtccc aggcgaggcc gatccaacgc aaagagcgct cgagctgctc
      721 gaccgactcc tccgtggagc gctccaggtc cgtgtcctct atccgcagca cgaacgtgcc
      781 gccgttcttg cgggcgaaga gccagttgaa gagcgccgta cgcacgccac cgacgtgcag
      841 cctgccagtc ggactgggcg cgtacctgac cctgatcttt gcgttgttct ccatgtgcta
      901 aacctagcac acgttagcct gggccgcgcg gtctgctccc cggttatacg tccttcaggt
      961 gatgctggcg cggacagcag gggcgtccgg cccgcgacgt atcgcctcct caacgcggca
     1021 cggactggct cctgtccagg taggagtagg cgagtccggt ggccgtcgcg tagacgacgt
     1081 ggtgcaggag gtccgtggcg atcgccttgc caccgtactt gaagacgggg gccgagacgt
     1141 ccagggtcgg gaggacgacc tgctcggcgc cccacaccag cccgaggtgg gccgcggtag
     1201 ccaggggacc ggatagcccg gccgaggcca agagcccccg cacgccgccc cagcccgtgc
     1261 cgtagcccca gtggaccagg ttggagaatc tggcttcgga atcgtcgtcc ttcggctcga
     1321 cgttcagcac cgcctcggcc gcctgggccg gggtctggct cgcctcgcgc ccgctgagct
     1381 tcatctccag cgtgctcgat accgtcatcg cggccgtgcc tgctgcaccc gcgaacaggc
     1441 ctttaccaaa atttgctgct acgtctccga tgctcatttc gatgcctcct ctcggtcggc
     1501 ttcgcttctt cggtcctcac cggacgagct tgtccggtgt tatgggaagg acgcggacgc
     1561 cggtggcgtg gtagacggcg ttggcgacgg cggcgggcga gccgacgatg ccgatctcgc
     1621 ctatcccttt ggaccccatc gggttgacgt gcgggtcctt ctcgtcgatc caggacacgt
     1681 cgatctcgcc cacatcggcg ttggtcggga tgtggtactc ggcgaagtcg tggttgacca
     1741 cgtgcccgaa acgctcatcg accaggctct cctcgtgtag cgccataccg aggcccatcg
     1801 tcatcccgcc gatgaactga gaacgggcgg tcttgacgtt gattactctg ccgaccgcga
     1861 agaccccggt catgcgcgga acgcgcacct ctccggtgtc ctcgttcacg cgcgcttcga
     1921 cgaagtgcgc cccgaaagcg tgcatcgagt agttctcggt ctccgggttt tcgggcgtcc
     1981 cggcctcgac cgtatcgcct tcggagggat cctcgccgta cctctcacgg aacacgcgtg
     2041 ccgcctcgaa aatggtcgaa ccccaggccg taatgcccga tgagccgccc gcgacggagg
     2101 ccgacggtag gccggtgtcc ccgatccgaa ggcctatctt ctctacaccc acgcccaggg
     2161 cgtcggcgga gatctgggtt agcgccgtcc acgtccccgt tcctatatcc acagcgccaa
     2221 tctctacggc gtagcgccct tcgtccagag agcgaatcct ggccttcgta cccggcatcc
     2281 tggtcaccgg atacatggaa gcggccaccc cggtcccgac gagccatcgt ccatccatcc
     2341 tcgcgcgagg tgccgggtcg cgcccctccc acccgaagcg ttccgctcct tcgcgcaggc
     2401 aggcgacgag gtttcggctg gagtagggta gccccgtatc cgcgtcgaac tcgggctcgt
     2461 tgcggatgcg gaactcgatg ggatcgaggt tgcacctcgc ggccatttcg tccatcgccg
     2521 cttcgagggc gtacatccct ggggcttcgc cgggggccct catccacgaa ggaacaggca
     2581 cgtcgagggc ggcgaggcgg tgcgtggtgc ggcgggtctg cgtggcgtac atcatcctcg
     2641 tcgcgtacgc ggtctgctcg gcgaactctt tgatcttcga ggtctgctcg atgacatcgt
     2701 gggagatcgc cgtgaggcgg ccatctccgt cggcgcctag ccgtatcctc tgcacggtcg
     2761 gggtgcggta cccgacgccg gagaacatct gctggcgggt gagcgcgaac ttcaccggcc
     2821 ggccctcgat aagctgcgcg gccagcgtcg tgaggacggt gttggcgtgg ggtaacccct
     2881 tcgacccgaa cccgccgccg acgtggggcg ccacgacccg tacccgatcc cggtcgagcc
     2941 cgaagaccct ggcgaccgcc ctcctgacgg tgtgaacccc ctgcgtagac tcgtacagtg
     3001 tgagatcccc ctcgtcccag acggcgaccg tggtgtgcgg ctccatgggg ttgttgtgct
     3061 ccatcggcgt cgtataggtc tcgtcgagct tgaccgtggc cgaggcgaac gcgtcatcga
     3121 agtcgccttc ctcggtatcg gtggggaagc cggcgttgag ctggtccggg gtgtaaaggt
     3181 cgtcaccctc cggtcgaagt tttgaatcgt gtgactcttc ggcgtatgag acctcgacca
     3241 ggttcgccgc gtgcctggcg acctcgaagg tctccgcgac tacggcgcct acgtactggc
     3301 cgcggaaagc gacttcgtcg gactgcaaga tcgcgagttc cttatcgctg gtgtccgcca
     3361 gccgcggcgc gttctcgtgg gtgacgacga tgaccacgcc aggaagagcc tcggcggcgc
     3421 tcgcgtctat actcgcgacg cggccccggg caatgtcgct ctgcagcgga tacaggtaga
     3481 ggggataccc gacacgatgc tcgtaggcgt agggggccgc tcccgtgacc ttctgtggcc
     3541 cgtcgatgcg atcgagaggt gcgccgattg ccctgcctgg cgcgcgaacg atcgtactca
     3601 tcgttcctcc gaaagctcga gcaacgtgcg ggtgatgatg ttgcgcgcca gcagtacttt
     3661 gaaggcgttc tcgcgcaagg gctcggcgtc ttcgagctcg atgtcggcgg cggcccggaa
     3721 gttcgcctcg ttggccggcg cgtccctgag cgcttgttcc gctttggtgg cgcgccaggg
     3781 cttgtgggcg acccctccca gcgcgaggcg cacgtcgcgg accgttcctt cttccacgtc
     3841 cagcaccgcg gcgaccgaga cgagggcgaa ggcgtaggag gcccgctcgc gtaccttgcg
     3901 gtactttgag ttgaacgcga acgggagcgg tggcaggtct gccgccgtga tgagctcacc
     3961 gtgctctaag gtcgtgtcgc gctggggttc gtcgccgggc aggcggtgca gttcctctat
     4021 ggggatccgg cgttcgccat catgccctcg caccagaacg aacgcgtcga gcgccgccat
     4081 cgcgacggcc atatcggagg ggtgggtggc gacgcagtgc tcggaggcgc cgaggatggc
     4141 gtggttcttg ttgtgcccct ctatcgcgga gcagccagat cccggctcgc gcttgttgca
     4201 gggcgtagtc gtgtcgtaga agtagacgca gcgcgtacgc tgcagcaggt tccctccggt
     4261 cgtggcgagg ttccggagct ggcccgacgc gccagagagc agagcttgcg agagcaccgg
     4321 gtagcgggag cggatggtcc tgtcggcggc aaggtcgctg ttggtgatcg cggccccgat
     4381 gcgcacaccg ccatcgggcg actcctcgat gctctccgag gtcagccgcc tcacgtcgac
     4441 gagtacgtct ggcgtcatca cctccagctt catcaggtcg accaggttcg taccgccggc
     4501 caggaacgct gcgtctggcg cttcggcgag cgcagcgacg gccccgccca cgtccctggc
     4561 gcgcacgtag ctgtagggtt tcaccgcccc gagacctccc taatagcagg gacgatggtc
     4621 gcgtaggcgg cgcaccggca gaggttaccg ctcatgcgct cccggatctc ctcgtcgtcg
     4681 aggactattt cttcgg
//
LOCUS       DATMVF010000229        11269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_625601, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000229 DATMVF010000000
VERSION     DATMVF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625601"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..410)
                     /locus_tag="VN178_11820"
     CDS             complement(153..410)
                     /locus_tag="VN178_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81701.1"
                     /translation="MGRSRGTGSQGFRLFMWIATALWLVGAIAAVIDRDTLNAVAWFG
                     FTAFGALTASGSTERSRGLAYLSIALLVVAMAILVGVFLAD"
     gene            complement(592..852)
                     /gene="rpsT"
                     /locus_tag="VN178_11825"
     CDS             complement(592..852)
                     /gene="rpsT"
                     /locus_tag="VN178_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWS81702.1"
                     /translation="MPAPSKRDKQSVKRGAHNKGVRTRLRNLSKSFYRAVESGDQGEA
                     QRIRDLSQKEYNKAAGKGIIHPNRAARKVSRLDKALATDRGE"
     gene            937..2715
                     /gene="lepA"
                     /locus_tag="VN178_11830"
     CDS             937..2715
                     /gene="lepA"
                     /locus_tag="VN178_11830"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWS81703.1"
                     /translation="MKNIERTRNFCIIAHIDHGKSTLADRLLDITEAVPERDRMDQIL
                     DTMDLERERGITIKAQAVRVLYNREDGEYNLNLIDTPGHVDFAYEVSRAIAACEGALL
                     IVDASQGIQAQTLANLYLAMEHDLEIIPVLNKIDLPIADVPAVTEELVELLGVEEEEI
                     IKISAKTGENVTGVLDAIVDRVPAPHTEHDETRALIFDSLYDPYRGVISLARLFDGEL
                     KKGDRVKAMGSEEHFELLEVGCYSPKPTALPVLGTGEVGYVIAGLKDIEALRVGDTVT
                     RTQAPASKPLPGYAKALPTVFSGLYPTEGDDFERLREALSKLQLNDASLFFEPENSRM
                     GFGFRCGFLGLLHMEIIQERLEREFDLDLIASSPSVKYEVVAGGDVVEVTNPSELPEI
                     FDEIREPIVRATVICPKEYVGAVMGLCHERRGVSVGMEYISTRRVQLTYDLPLAEIIT
                     DFFDAMKSRTKGYASYDYEPLGYQPSDLVKVEVLVSGETVDALSIIVHCDKAYQRARA
                     LVEKLKELIPRQQFEVPVQATMGKRVIARETVRAYRKDVTAKCYGGDITRKRKLLEQQ
                     KKGKRRMKQVGSVEIPQEAFLAALRI"
     gene            2991..4019
                     /gene="hrcA"
                     /locus_tag="VN178_11835"
     CDS             2991..4019
                     /gene="hrcA"
                     /locus_tag="VN178_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWS81704.1"
                     /translation="MKHLELSERQRELLLKTLEIYISTGAPVGSHALSDELGASSSTI
                     RAELAHLESLGLLDHPHTSAGRVPTDSGYRYYVDALMEASPKDDTAFAALEGYGNVDE
                     LLRGVSEGMSEVTRLLAVVAGPSVLGESLSRVDYLPLGEGRLLLVVSMESGASSSAAI
                     TLPVPVEDDELRHIFAALNSWLGGRPLGTDLELGLAARTALSDHDPMTVGAVLEAVGA
                     LGRATERGVFIQGASALLSRLDDLDPGSLSAVIEIFERRRWLLRLMGDALRRSVCSSG
                     VVVSIGTESGFLNLMGTSLVASAYSRHERPYGVVSLIGPKRMDYDAAITTVRSAAEAL
                     TNCLDARF"
     gene            4020..5156
                     /gene="dnaJ"
                     /locus_tag="VN178_11840"
     CDS             4020..5156
                     /gene="dnaJ"
                     /locus_tag="VN178_11840"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWS81705.1"
                     /translation="MATKRDYYEILGVSREASDAEIKKSYRRLARSHHPDANPGDHTA
                     EERFKELTEAYEVLSNPEARRAYDTYGHQVPRGAGAAGYPGAGDPFGGFQDIFEAFFG
                     DRSFGGSFFGASTAQGPSRGSDAEVEVEVSLYEAAFGADREVNVQVVSNCGVCDGIGG
                     TESHVCPTCSGAGSVRTVRESILGQMVTTQSCSTCGGRGRVIDVVCDNCRGSGRVSEV
                     QTRRLRVPEGIESGMRLRVSGGGHAGDPGAPPGDLYVRINVREDEELMRDGEDLIHRM
                     RVSFVDAALGAEVEVPTLEGPRRVRVDPGTQPGATLTLRGDGMPRLRRRGRGDLKVVV
                     DVMVPTRLTSEQRDLLERFEATTGEETYNGSGGSFFDRLRGVFR"
     gene            5222..5881
                     /locus_tag="VN178_11845"
     CDS             5222..5881
                     /locus_tag="VN178_11845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWS81706.1"
                     /translation="MLELPPGETNHVRNVLRGRNGDFMEVVDGGGRLFTARLRRGGEA
                     AVVEELAAPDGVGVEITLYQAVPKGGRMDLLVEKATEVGATRIVPLLAERGVVSPRGG
                     KVGRWRRVAEAAARQALRLGVPDVAEPVRFEEAASEVKGTGILLHNAPDLQPVEAVVG
                     APASLFVGPEGGWSAGELRLAEEVGITFGGLGPYRLRSETAGIIAVARTLAALESMVE
                     R"
     gene            5893..6237
                     /locus_tag="VN178_11850"
     CDS             5893..6237
                     /locus_tag="VN178_11850"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HWS81707.1"
                     /translation="MSEADCIFCRIVRGEIDAEVVHDEDDVLAFEDINSKAPVHVLVV
                     PRQHVANLEEIGAMPDTVVKRLFEVASEVAQKLGVTEGGYAVRINNGRDAGQEVFHLH
                     LHVMGGKKLGMP"
     gene            6234..6677
                     /locus_tag="VN178_11855"
     CDS             6234..6677
                     /locus_tag="VN178_11855"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWS81708.1"
                     /translation="MTISDRIQRDTRDAMKQRDRARVGALRMLGAALKNAEIEAGRPL
                     TEQEEQTILRRQLKQRDESAEAFRKAGREERAASESAEAEIVRFYLPEPPSQDELEEI
                     IDRAVSETGASGMKDMGAVMARAMELSEGRAEGRTISALARGRLQ"
     gene            6931..7812
                     /locus_tag="VN178_11860"
     CDS             6931..7812
                     /locus_tag="VN178_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWS81709.1"
                     /translation="MLRRVEELVECEVVVRGNEIVLRGTEEAVERAEAVFDGLVGLAE
                     EGHHPTPETAERLYKMSDEGGGREVLGDVILTHRGRRVAPKTRNQKAYTDAIRQHQVV
                     FGIGPAGTGKTYLAVALAVEALNRGEVSRIILTRPAVEAGESLGYLPGDVMAKVDPYF
                     RPLYDALYEMVDPAKFQAHLERGIIEIAPLAFMRGRTLNDAFIILDEAQNTTPPQMKM
                     FLTRLGFGSKMVITGDITQVDLPNARISGLQDARNTLTGVEGVSFVGLRRDDIVRSDL
                     VMRIVDAYEGAGEGDRA"
     gene            7834..9417
                     /locus_tag="VN178_11865"
     CDS             7834..9417
                     /locus_tag="VN178_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWS81710.1"
                     /translation="MKLGKRSLKKQPGRRVDELHRSPTRFELFRAWIERIPKVRLYIG
                     LTALTWLSLTALIGIAPRPLIERVISQGIIDSSDRTRLESLDLLQQSDPWMALVGVGI
                     VVAAEMWIAWYFLERFGERILKTNSAIRLVLAASLTILFTALARFFIELEFDPYLTPL
                     AGLSIIGTMLLGPRLMFLMVVITSVNVGILSGNNFLLTAALLLGSGFAIYSTVRVDSR
                     QRLLKAGLVIAAVMGVVTFAVGLIGGGDLRDALWLGVFGLGNGLLSLMLATVLLPFLE
                     DAFNILTPMKLLELSDPRHPLLDKLLRNAPGTFSHSMQVGLLAERAAERVGADALLAQ
                     VGAYYHDIGKMEHPAYFVENQIAQINPHAALSPTLSAKVVKRHVKDGLEIGDAWGLPH
                     EILDIIAQHHGSTRIEYFYRKALEDAPDEDAVRESDFRYSSGLPRSKEAGIMMLADSI
                     EATVKSLPKPTPKRIDDVVADTVRRKLQDGQFDECQLTMHDIHEAGEAIREALIGFLG
                     PRIAYPEDSGDTRNGKAAT"
     gene            9465..9875
                     /gene="ybeY"
                     /locus_tag="VN178_11870"
     CDS             9465..9875
                     /gene="ybeY"
                     /locus_tag="VN178_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWS81711.1"
                     /translation="MSVAVLDEVGHAGLDTKRASILFAAALSGLGLDLERIGEVSVAL
                     VDPETIRNLNLEFRDRDEPTDVLSFEVDGPYGEMVGEVVICPECAHRGMGVDELVVHG
                     ALHLGGMDHGEDFGASEMARLQRAVMEELRGEEG"
     gene            9859..10602
                     /locus_tag="VN178_11875"
     CDS             9859..10602
                     /locus_tag="VN178_11875"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HWS81712.1"
                     /translation="MARKVERPDKGQQGVARSFEHAYRGMIYAVRTQRNMRFHVIVAV
                     LVLVASLLVGVRKLELAVLVLTILLVFVTELFNTALEFAVDLATKEYHPLAKLAKDIS
                     AGAVLVSSVGAVLVGYLVLADDLGPLSLATLETIRRQPAHLTLVTLGVIVLVVLLGKA
                     LTHSPNSFAGGMPSGHAAVAFAGWVAASFIAVQGGAGSYAGLISVISLLMALLVCQSR
                     VESGLHSVYQIIMGAVIGVLVTTAAFQLL"
     gene            10614..11003
                     /locus_tag="VN178_11880"
     CDS             10614..11003
                     /locus_tag="VN178_11880"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWS81713.1"
                     /translation="MDPEIVMQAARDAAKRAYAPYSDFHVGAAILTGGSTIHQGANVE
                     NASYGLSMCAERNAVAMMAATDPEDREIELVAVFSPNASPCFPCGACRQVLREFGCKE
                     VLVETGRGLQRYPFDQILPNSFGPEDL"
     gene            11012..>11269
                     /locus_tag="VN178_11885"
     CDS             11012..>11269
                     /locus_tag="VN178_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWS81714.1"
                     /translation="MTRDVANNGFRSGFIAVVGRPNVGKSTLVNRLVGQKVSITSPRP
                     QTTRSPIRGVRNGPGYQAIFVDTPGSQKPRDTLRARMQEQVE"
BASE COUNT         2179 a         3513 c         3627 g         1950 t
ORIGIN      
        1 tgtgcgagtc ctacctcgtg taagaagtgc acccggtgga gagcctccgc aacgcctcca
       61 ctttggtcgg gtggccacct tcgcccatgg tcatctccct tttatgacca tcgcccgcaa
      121 gtgttcgtta gaacacggag cagctccaga atctagtctg cgaggaaaac acccaccagg
      181 atcgccatgg cgacgaccag gagcgcgatg gacaggtaag ccaatcccct ggagcgttcc
      241 gtgctaccgc ttgcggtcag ggccccgaac gcggtgaagc cgaaccaggc tacggcattg
      301 agcgtatcgc gatcgatgac cgcagcgata gccccgacga gccacagcgc cgtggctatc
      361 cacatgaaga gcctgaaacc ctggctgcct gtccctctac ttctgcccat cctctaaccc
      421 tccgggtgtc tgatgctgga gcacagcaag tatttcatct tctgcgccgt cccgctattt
      481 acctgcattc actaaaggta accaggcggt agccacgcat acaagagagc aggaaagcga
      541 ctggatgccc agacgggcag gatcctcgat ctcagtctct agtcgaggtg gttactcgcc
      601 ccggtcggtc gcgagtgcct tgtcgaggcg gcttaccttg cgggcagccc ggttcggatg
      661 gattatgcct ttgcccgcgg ccttgttgta ttccttctgg gacaagtccc ggatgcgctg
      721 ggcctcccct tggtcacccg actcgacggc cctgtagaag ctcttcgaga ggttgcgaag
      781 gcgggtcctg acgcccttgt tgtgggcgcc acgcttgacg ctctgcttgt cgcgcttgga
      841 tggtgcgggc acgctggctc ctcggatctg atacttacct gaccggaaga atgtaacaca
      901 aggagagcaa agcggcaatc atggtagaat ctcccggtga aaaacatcga gcgtactcgc
      961 aatttctgca tcatagcgca tatcgaccac ggtaagagta ccctcgccga caggctactc
     1021 gacataacgg aggccgtccc cgagcgcgat aggatggacc agatcctcga caccatggac
     1081 ctggagcgcg aacgtggcat cacgattaag gcccaggccg tgcgcgtcct ctacaacagg
     1141 gaagacggcg agtacaacct gaacctgatc gacacgcccg gtcacgtcga cttcgcctac
     1201 gaggtctctc gggccatagc ggcctgtgaa ggcgcgctcc tcatcgtgga cgcgagccag
     1261 ggcatacagg cccagaccct cgcgaacctc tacctggcga tggagcacga cctcgagatc
     1321 atccccgtgc tcaacaagat agacctcccc atagccgatg ttccggccgt cacggaggaa
     1381 ctcgtggaac tgctcggcgt ggaagaggag gagatcatca agatctccgc caagaccggc
     1441 gagaacgtaa caggggtgct cgacgcgatc gtggaccgcg tccccgcgcc gcacaccgag
     1501 cacgacgaga cgcgggccct catcttcgac tccctctacg acccttaccg gggcgtcatt
     1561 tcactggcgc gtctgttcga cggggaactg aagaagggcg acagggtcaa ggcgatgggg
     1621 agcgaggagc acttcgagct tctggaggtc ggctgctact caccgaaacc cacggccctc
     1681 cccgtactcg gaacgggcga ggtgggatac gtaatcgcag gcctcaaaga catcgaagcc
     1741 ctacgcgtcg gcgatacggt cacgcggacg caggcgcccg cttctaaacc gctgccgggc
     1801 tacgcgaagg cgttgccgac ggtcttctcc ggcctctatc ccacggaggg cgacgacttc
     1861 gaacggctaa gggaggccct ctccaagctg cagctaaacg acgcctcgct cttcttcgag
     1921 cctgagaatt ccaggatggg tttcgggttc aggtgtggtt ttctgggcct cttgcacatg
     1981 gagatcatcc aggagcggct cgagcgtgag ttcgacctcg acctcatagc gtcctccccg
     2041 agcgtgaagt acgaggtcgt cgccggaggc gacgtcgtcg aggtcaccaa tccgagcgag
     2101 ctaccggaga tcttcgatga gatcagggag cccatagtgc gcgcgaccgt gatctgcccc
     2161 aaggagtacg tgggggccgt gatggggctc tgccacgaga ggcgcggggt ctccgtcggg
     2221 atggagtaca tctcgacccg cagggtgcag ctcacctacg acctgccgct ggccgagatc
     2281 atcacggact tcttcgacgc catgaagagc cgcacaaaag gctacgcctc ctacgactac
     2341 gagcccttgg gctaccagcc ttccgacctg gtgaaggtcg aggtcctcgt ctccggtgag
     2401 accgtcgatg cgctctccat catcgtccac tgcgacaagg cctatcagcg cgcccgcgcc
     2461 ctcgtcgaga agctcaaaga gttgatccca cgccagcagt tcgaggtgcc ggtccaagcc
     2521 accatgggca agcgcgtgat cgcccgcgag accgtccgcg cttaccgcaa ggacgttacc
     2581 gccaaatgct acggtggcga cataacccgt aaacgcaagc tcctcgagca acagaaaaag
     2641 ggcaagcgcc gcatgaagca ggtcggtagc gtggagatac cccaagaagc cttcctggcc
     2701 gcgctacgca tctagcacgc tccggctttt agcctccgct tgtcagcccg gcggctgcgc
     2761 cgcctcgtca gcacgcttcg ggccttgcaa gccctcgctt gtcagctagt cagcttcttc
     2821 cgttctggcc tcgctcgaca gcgcacgaga ggtgcattcc cccacggtaa cgtttacgct
     2881 catggcgagt ctacgggacg acttctgaaa tgctgaagtg ctgacaagcg aaggcgaagc
     2941 ctgagcgtgc tgaaatgctg agaggtaccc atgttagaat cacgtacgac atgaagcacc
     3001 tcgagctctc agagagacaa cgcgagctac tgctgaagac gctggagatc tacatctcca
     3061 cgggcgcccc tgtcggaagc catgccttat ccgacgaact gggcgcgagc agctccacga
     3121 tccgtgcgga gctggcccac ctggagtcgc tgggcttgct cgaccatccg cacacctcgg
     3181 ccggaagggt ccctacggac agcggctacc gctactacgt cgacgccctg atggaggcga
     3241 gtccgaagga tgacacggcg ttcgcggccc tggaaggcta cggcaacgtg gacgagctgc
     3301 tgcgtggcgt ctcggaggga atgagcgagg ttacccgcct tctggccgtg gtcgcggggc
     3361 ccagtgtgct gggcgagtcg ctctcccgtg tcgactatct gcctctgggc gaaggaaggc
     3421 ttttgctcgt cgtatcgatg gagagcggcg cctcttcgag cgccgcgatc actctgcccg
     3481 tccccgtcga ggatgatgaa ttgcgccaca tcttcgcagc cctcaactcc tggctgggag
     3541 gtcgcccgct cggcaccgac ctggaactcg gtctcgccgc gcgcacagcg ctctcggatc
     3601 acgacccgat gacggtgggg gccgtcctcg aggccgtagg cgcgctggga agggctactg
     3661 agcgcggggt cttcatccaa ggcgcctcgg ccctcctcag ccgcctggac gacctcgacc
     3721 cgggcagcct ctcggccgtg atcgagatct tcgaacggcg gcgctggttg cttcgcctga
     3781 tgggcgacgc cctccgtcgc tccgtttgca gcagcggggt cgtcgtctcc atcggcacgg
     3841 agagtggctt cttgaacctg atgggcacga gcctcgttgc ctcggcctac agccgccacg
     3901 agcggccata cggggttgtc agcctgatcg gccccaagcg catggattac gacgccgcga
     3961 tcaccacggt ccgctcggcc gcggaggccc tgaccaactg tctggacgcc aggttctagt
     4021 tggcaaccaa gcgcgactac tacgagattc tgggcgtgtc cagggaagca tcggacgcgg
     4081 agatcaagaa gtcttaccgt cgcctcgccc gctcccacca ccccgacgcg aaccccggcg
     4141 atcatacagc cgaggaacgc ttcaaagaac taaccgaggc ctacgaggta ctctcgaacc
     4201 cggaagccag acgcgcctac gatacatacg gccaccaggt accgcgcggc gccggagcgg
     4261 caggctaccc aggcgccgga gacccgttcg gtggcttcca ggacatcttc gaagctttct
     4321 tcggcgaccg ctctttcggc ggctccttct tcggtgcctc caccgcgcag ggtcccagtc
     4381 gtggaagcga cgccgaggtc gaggtagaag tctccttgta cgaagcggcg ttcggggctg
     4441 accgtgaggt caacgttcag gtagtcagca actgtggcgt ctgcgacggt atagggggaa
     4501 cggagtcaca cgtgtgtccc acttgcagtg gcgccgggtc ggtaaggacg gtgcgggaga
     4561 gcatcctcgg gcagatggtg acgacccaat cctgctcgac gtgcggcggc cgcggccgcg
     4621 tcatcgacgt ggtgtgtgac aactgtcgcg gcagcgggag agtctccgag gtacagacga
     4681 gacggctgcg ggtgcccgag ggtatcgaga gcggaatgag gctcagggtc tcgggtgggg
     4741 ggcatgcagg agacccgggc gcgccaccgg gggatctgta cgtgaggata aacgttagag
     4801 aagacgagga gctgatgcga gacggggagg acttgatcca ccgcatgagg gtcagcttcg
     4861 tcgatgcggc gctcggggcc gaagtggaag tgccaaccct cgaaggaccg agacgggtga
     4921 gggtggatcc cggcacccag ccgggtgcga cgctcacgct caggggcgat gggatgccgc
     4981 ggttgcggcg gcgtgggcgc ggagacctca aggtggtcgt cgacgtcatg gtaccgacgc
     5041 gtctgacgtc tgaacagcgg gatctgctcg agaggttcga ggcaactacc ggcgaagaga
     5101 cttacaacgg tagtggaggc tccttcttcg accgcctcag gggtgtcttc cggtaggagg
     5161 cttcgttacg cgcacgccga taaccagaat cttcatcgac tccgaactgg agccgggcgg
     5221 agtgctggag ctgccaccgg gtgagaccaa ccacgtccgc aacgtcctgc ggggcaggaa
     5281 cggggatttc atggaggtcg tggacggcgg ggggaggctc ttcactgcca gattgcgcag
     5341 gggcggagag gcggccgtgg tggaggagtt ggcggcgccg gatggagttg gggtggagat
     5401 taccctctac caggcggtgc ccaagggggg aaggatggac ctcctcgtcg agaaggccac
     5461 ggaggtcggg gccacgcgta tcgtgccgct gttggccgag cgcggcgtcg tgagcccgcg
     5521 tggtgggaag gtcgggcgct ggcggcgggt ggccgaggcg gccgcgcgcc aggctttgcg
     5581 gctcggcgtg cccgatgtgg ccgaacccgt acgattcgag gaagccgcct cggaggtgaa
     5641 agggaccgga atcctactcc acaacgcccc cgacctccag ccggtcgagg ccgtagttgg
     5701 ggctcctgcg agcctctttg tgggcccgga gggtgggtgg agcgcgggcg agttgcggct
     5761 cgctgaggag gttggcatca ctttcggcgg gttgggaccg tacaggctcc gcagcgagac
     5821 cgcaggtatc atcgcggtgg cgcgtacgct ggctgcattg gagagcatgg tcgaacgtta
     5881 ggggaggtgg ccgtgagcga ggccgactgc atattctgta gaatagtgcg aggggagata
     5941 gatgccgagg tggttcacga cgaggatgat gtgctggctt tcgaagacat aaatagcaag
     6001 gcgcccgttc atgtcctcgt cgtccccagg cagcacgtcg cgaacctgga ggagatcggc
     6061 gcgatgcccg ataccgttgt caagcggctc ttcgaggtgg ccagcgaggt cgcccagaag
     6121 ctcggcgtaa ccgagggcgg atacgccgtc aggatcaaca acggccgcga cgccggtcag
     6181 gaggtcttcc acctccacct gcacgtcatg ggcggcaaga agctgggaat gccgtgacca
     6241 tatcggaccg catacagcgc gacaccaggg acgccatgaa gcaacgcgac agggcccgcg
     6301 tcggcgccct gcgtatgctc ggcgcagcgc tcaaaaacgc cgagatagag gcgggaaggc
     6361 cgctcaccga gcaggaggag cagaccatcc tcaggcgcca gctcaagcag cgcgatgagt
     6421 ccgcagaagc gttccgcaaa gccggacggg aggagcgggc ggcctccgag tcggccgaag
     6481 cagagatcgt ccgcttctac ctccccgagc caccctcgca agacgaactc gaagagatta
     6541 tcgaccgggc ggtgagcgag acaggagcaa gcggcatgaa agacatggga gccgtcatgg
     6601 cgcgtgccat ggaactctcg gagggccgcg ccgaaggccg caccatttcc gcgctggcgc
     6661 gaggtcgact gcaatgagga aggcttcagg ctacaggttt caggcatcag gaggtgcaga
     6721 tctcacccca agcgggagca aagatcggct acccaaagaa ggcgtaggta cacgcgtccc
     6781 cgcctcgccg tccgacgaga acctgaaacc tgatgcctga aacctgacgc ctataaagga
     6841 gaagacacgg aaacaaaaag cagccaagtc gaagtcaaac tcgtcatacc gccaggcagg
     6901 actctcgacg tcttcgggga ccgcgactcc gtgctgaggc gggtcgagga gctcgtcgag
     6961 tgcgaggtcg tagtgcgggg taatgagatc gtactgcgcg gtaccgagga ggccgtggag
     7021 agggccgagg ccgtcttcga cggtctggtc ggcctcgccg aggagggtca tcacccgacc
     7081 ccggagaccg ccgagcgact ctacaagatg tccgatgaag gcggcggacg agaggtgctg
     7141 ggggatgtga tcctcacgca ccgagggcgc cgggtcgcgc cgaagacccg caatcagaag
     7201 gcgtacaccg atgccattcg ccagcatcag gtggtcttcg gcatcggacc ggcgggcacg
     7261 ggcaagacgt acctggccgt ggccctggcc gtcgaggcct tgaacagggg agaggtgtcc
     7321 aggatcatac tgaccaggcc cgctgtagag gccggcgagt ccctcgggta cctgccgggc
     7381 gacgtgatgg ccaaagtgga tccctacttc aggcccctct acgatgcgct ctacgagatg
     7441 gtcgacccgg cgaagttcca ggcgcacctg gaacgaggta taatcgagat agcacccctc
     7501 gcattcatgc ggggtcgtac gcttaatgat gcgtttatca tcctggatga agcgcagaat
     7561 accacccccc cgcagatgaa gatgttcctg acgcgactgg ggtttggatc caagatggtc
     7621 attacgggcg acattacgca ggtggatctc ccgaatgccc gcataagcgg gctgcaagac
     7681 gccaggaaca ccctcactgg cgtagaaggc gtatcattcg taggactgag gcgcgatgat
     7741 atcgtgcgta gcgacctcgt catgcgtata gtcgatgcct atgaaggggc gggagagggc
     7801 gacagagcct aggcccaagg gccgggagcg accatgaaac tcgggaagag aagcctcaag
     7861 aaacagcccg gcaggcgtgt cgacgaactg caccgctcgc cgacgcgctt cgagctcttc
     7921 agggcctgga tcgagaggat cccgaaggtc aggctctaca tcgggcttac tgccttgacg
     7981 tggctctctt tgacggcgct cattggtata gcgccgcggc ccctgatcga acgcgtcatc
     8041 tcccagggga tcatagactc gagcgaccgc acgcggctcg agtctctgga cctgctgcag
     8101 cagtcggacc cgtggatggc gctggtcggg gtcggcatcg tcgtcgccgc cgagatgtgg
     8161 atcgcctggt acttcctgga acgcttcggg gagcgcatac tcaagacgaa ctcggccata
     8221 aggctcgttc tcgccgcctc gctcactatc cttttcaccg cgctcgccag attctttatc
     8281 gagctggaat tcgatcccta cctcacgcct cttgcgggtc tctccatcat cggaaccatg
     8341 cttctcgggc cgaggctcat gttcctgatg gtggtgatca ccagcgtcaa cgtaggtatc
     8401 ctcagcggca acaacttcct cctgaccgcg gccctgctgc tgggatccgg tttcgccatc
     8461 tactccaccg tgcgagtgga ctcccgccag cggttgctga aggccgggct ggtcatcgct
     8521 gccgtcatgg gcgtcgtgac gttcgccgtg ggtttgatcg ggggcggcga tctgcgagac
     8581 gccctgtggt tgggtgtctt cggactcggc aatggcctcc tctcgctgat gcttgcgacg
     8641 gtgctgttac ctttcctgga agacgcgttc aacatcctca cgccgatgaa gctcctggaa
     8701 ctctccgacc cccgacatcc tctgctcgat aagctactca gaaacgcacc aggcactttc
     8761 tcccactcca tgcaagtcgg cctgctcgcc gagagggccg cagaaagagt aggcgccgac
     8821 gcgctactcg cccaggtcgg cgcctattac cacgacatcg gaaagatgga acatcctgcc
     8881 tacttcgtgg agaaccagat cgcccagatc aacccgcacg cagccctctc cccgacgctc
     8941 tcggccaagg tggtcaaacg ccacgtaaag gacggcctgg agataggtga cgcgtggggc
     9001 ctgccccatg agatcctgga cataatcgcc cagcaccacg gatcgacccg catcgagtac
     9061 ttctaccgca aggccctcga agacgccccc gacgaggatg cggtccgcga gtcggacttt
     9121 cgctactcca gcgggctgcc ccgcagcaag gaggctggca tcatgatgct cgccgactcc
     9181 atagaggcga ccgtgaagtc tttgccgaag ccgaccccga agcgtatcga cgacgtcgtc
     9241 gccgacacgg tgcgaagaaa gctccaggac ggacagttcg acgagtgcca gctcaccatg
     9301 cacgatatcc acgaggcagg ggaggccatc cgcgaggcgc tcataggttt cctcggtccg
     9361 cgcatcgcgt atcccgaaga ctcgggcgat actcgcaacg gaaaggcggc gacgtaggcc
     9421 gtggacgcta cggacaggat cgccacctga ggaactcccc tcctatgtcc gtcgccgttc
     9481 tcgatgaggt tggccatgcc ggactcgaca caaaacgagc cagtatcctc ttcgccgccg
     9541 ccctctccgg cctcggtctc gacctggagc gaatcggcga ggtctccgtg gcactggtcg
     9601 accctgagac gatccgtaac ctcaacctag agttcagaga tagagacgaa cccaccgacg
     9661 tgctcagctt cgaggtagac gggccatacg gggagatggt aggggaggtg gtgatctgcc
     9721 ccgagtgcgc ccatcgtggg atgggcgtcg acgaactcgt ggtgcacggc gcgctacact
     9781 tgggcggcat ggaccacgga gaggacttcg gggccagcga gatggcgagg ctccagcggg
     9841 ccgtaatgga ggagttgcgt ggcgaggaag gttgagcgac cggacaaggg tcagcagggc
     9901 gtggcccgca gcttcgagca tgcctaccgg ggtatgatct acgcggtccg cacccagcga
     9961 aacatgcgct tccacgtcat cgtggccgtc ctggtcctcg tggcgagtct gctggtcggt
    10021 gtcaggaagc tggaactcgc ggtcctcgtt ctgaccatac tcctggtctt cgtgaccgag
    10081 ctattcaaca cagctctgga attcgccgtc gacctcgcca caaaggaata ccatcctctt
    10141 gccaagctcg ccaaggacat ctcggcaggg gccgtcctgg tctccagcgt cggggcggtg
    10201 ctcgtcggtt atctcgtcct ggcggacgat ctggggccgc tctctctcgc gaccctggag
    10261 accataaggc gccagccggc ccacctcacg ctcgtcaccc tcggcgtgat cgtactagtc
    10321 gtcctactag gcaaggcgtt gacccactcc ccgaactcct tcgccggcgg gatgccctcc
    10381 gggcacgcgg cggtcgcctt cgcggggtgg gtcgcagcga gcttcatagc cgtccagggc
    10441 ggcgcgggat cctacgccgg gctcatctcc gtgatctcgc ttctcatggc cctgctcgtc
    10501 tgccagagcc gcgtcgagag cgggcttcac agcgtctacc agatcattat gggggccgtg
    10561 ataggggtcc tcgttacgac ggcagccttt caactcttat gaagggagga gccttggacc
    10621 ctgagatcgt gatgcaggcc gcccgcgacg ccgcaaagcg ggcttacgcg ccatacagcg
    10681 acttccacgt cggagcggcc atcctgacag gaggaagtac catacaccag ggcgcgaacg
    10741 tcgagaacgc ctcctacggg ctctcgatgt gcgcggagcg caacgcggtt gcaatgatgg
    10801 ccgctacaga ccctgaagac agggagatcg aactcgtcgc cgtcttcagc ccgaacgcct
    10861 cgccctgttt cccctgcgga gcctgccggc aggtgttgcg ggagttcggc tgcaaagagg
    10921 tcctcgtgga gaccggccgc gggctacagc gctacccctt cgaccagatt cttcccaatt
    10981 ccttcggccc ggaggacctt tgaaggtcgc tatgacccga gacgtcgcaa acaacggatt
    11041 ccgcagcggc ttcatcgccg tggtaggcag gcccaacgtc ggcaagagca ccctggtcaa
    11101 caggctcgtc ggccagaaag tctccatcac ctcgcccaga ccgcagacca cgcgcagccc
    11161 gatccggggc gtccgcaacg gacctggata ccaggcgatc ttcgtggata ccccgggctc
    11221 acagaagccc cgcgacacac tgcgggcgag gatgcaggaa caggtcgag
//
LOCUS       DATMVF010000230         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_672778, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000230 DATMVF010000000
VERSION     DATMVF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672778"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="nusB"
                     /locus_tag="VN178_11890"
     CDS             complement(<1..327)
                     /gene="nusB"
                     /locus_tag="VN178_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWS81715.1"
                     /translation="MSRRTARKQAFLNLYQSDVNDCPLGPTLERWREYRGNLQEYAVS
                     LARGVEQEQEQLDALLSEVSVGWPVHRMSAVDRSILRLALYEMLFVDDVPPEVAVNEA
                     VELAKGF"
     gene            complement(351..908)
                     /gene="efp"
                     /locus_tag="VN178_11895"
     CDS             complement(351..908)
                     /gene="efp"
                     /locus_tag="VN178_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWS81716.1"
                     /translation="MISTNQFRNGTAIRVDGKRFTILFFQHVKPGKGGAFVRTRLRNI
                     DSGAVVEKTFRAGEKLENVRTESRPMTYLYRDGDLLYFMDSDTYDQVPVTVDVVGEAA
                     GYIVEGGTVSVLSADGEVVSVEPPAHVDLGVAQTDPGLKGDTATGGTKPATLETGVVV
                     QVPLFVNVGDRVRVDTRSNEYLTRV"
     gene            complement(1080..1505)
                     /locus_tag="VN178_11900"
     CDS             complement(1080..1505)
                     /locus_tag="VN178_11900"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWS81717.1"
                     /translation="MSKVFVLNGVNLGALGTRRPEVYGTMTLDDIERLVKDEFPALDL
                     RFRQTDYEGEMVGFIREAAGCDGLVINAGAWTHYSYALHDALEALDVPKVEVHLSHVG
                     AREEWRRVSVISPAVDAVIAGMGPHGYVAAVRYLLARGT"
     gene            complement(1498..2583)
                     /gene="aroB"
                     /locus_tag="VN178_11905"
     CDS             complement(1498..2583)
                     /gene="aroB"
                     /locus_tag="VN178_11905"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWS81718.1"
                     /translation="MPVETVAVPVTQPYRVFIGAGIDLAEIFSGSLEPGGCAIFTDST
                     VGPLHAARVRNALQSAGWRVAGKIELPAGESSKSLDVYADSLGRLARMGLTRDGTIFA
                     LGGGVVGDLAGFVAGTYMRGIDLVMLPTSLLAMVDSAVGGKVGIDLPEGKNLVGAFVR
                     PRLVAADLDWVATLPEREISNGLAEAIKMGLLSGGDFFGDLGLIGAALTGDLAALQTL
                     VLDSVGFKARVVAEDELEGGLRQVLNYGHTIGHALEAAAGYKLQHGRAVAAGMVAAAS
                     LSKERFGRDLRSVHEDLLSAAGLPLRVPDVGVERALLAMGRDKKRSAADGPSWHRFVL
                     LEDIGKPVRSVPVSEEEVREAIGAVVE"
BASE COUNT          477 a          944 c          833 g          483 t
ORIGIN      
        1 gaatcccttt gccagctcga cggcctcgtt taccgcgact tcggggggga cgtcgtccac
       61 gaagagcatc tcgtagagcg cgaggcgaag gatagaacgg tctaccgcgc tcatgcggtg
      121 aaccggccac cccaccgaga cttccgagag caaagcgtcg agctgttcct gctcctgctc
      181 cacgccgcgc gcgaggctga cagcatactc ctggagattg cccctgtact cccgccagcg
      241 ttccagggtc gggccgagag gacagtcgtt tacgtcgctc tggtacaggt tcaagaacgc
      301 ctgcttgcgg gccgtccggc ggctcaaggg cctctccagt ccaccgcgct tcagaccctc
      361 gtcaggtact cgttggagcg cgtgtccacc cttacccggt cgcccacgtt gacaaagagc
      421 ggaacctgca ccacgacgcc cgtctccagg gtggccggct tggtcccccc cgtcgcggta
      481 tcgcccttga ggccggggtc ggtctgcgcg accccgaggt ccacgtgcgc gggcggctcc
      541 acgcttacga cctcaccatc ggccgaaagc acggagacgg tgccgccctc gacgatgtac
      601 cctgccgctt cgccgacgac gtccacggtg accggcacct gatcgtaggt atccgagtcc
      661 ataaagtaga gcaggtctcc gtccctgtag agatacgtca taggacgcga ctcggtcctc
      721 acgttctcga gcttctcccc cgcacgaaag gtcttctcta ccacggcccc actgtctatg
      781 ttcctgagcc gcgtccttac gaatgccccg cccttgcccg gcttgacgtg ctggaaaaac
      841 aggatcgtga accgcttgcc atcgacccgg atggccgtac cgttcctgaa ctggttggtg
      901 gatatcatgg tcggaagctc ccgttcacgg cgtctgatct tctctgcgga cctgcgcggc
      961 gaaaacccgg cacagacgtc gggctattat cgcacagttt ctttccctgt cagccttctt
     1021 atgcgccggg acaatagcac cgaagatgac cctctcatag cctgtgagac ggcccctgat
     1081 caggtccccc gggcgagaag gtatctgacc gcggcgacgt aaccgtgggg gcccatgccc
     1141 gcgattaccg cgtccaccgc cggtgagata accgacaccc ggcgccactc ctccctggcg
     1201 cccacgtgtg acagatgaac ttcgaccttt ggtacatcca gggcttccag ggcgtcgtgc
     1261 aaggcgtaag agtagtgggt ccaggcaccg gcgtttatga cgagcccgtc gcagcccgcc
     1321 gcctccctta tgaaaccgac catctcgccc tcgtagtccg tctgcctgaa ccggaggtcc
     1381 agagccggaa actcgtcttt gacgagcctc tctatatcgt cgagggtcat ggtcccgtag
     1441 acctcgggac ggcgggtacc gagggcgccg aggttcacgc cgttgagaac gaagacctta
     1501 ctcaacgacg gctccgatcg cctcgcgaac ttcttcttcg ctcacgggca cgctccttac
     1561 gggtttgccg atgtcttcga gtagcacgaa tctgtgccag gagggtccgt ctgcggcaga
     1621 gcgtttcttg tcgcggccca tcgcgagcag cgcgcgctcg acgccgacat cgggtacgcg
     1681 gagcggcaaa ccggcggcgg ataacaggtc ttcgtgtacg cttctcaggt ccctgccgaa
     1741 acgctctttg gagaggcttg cggccgcgac cattccggca gcgaccgccc ggccatgctg
     1801 cagcttgtac ccagcggcgg cctccagagc gtggccgatg gtgtggccgt agttcaggac
     1861 ctggcgcagc cccccttcca actcatcttc ggcgaccacg cgcgccttga agccaacgga
     1921 atcgaggacc agagtctgca gcgcggcgag gtcgcctgtt agggcggccc cgatcaggcc
     1981 gaggtcgccg aagaagtctc ctcctgagag tagccccatc ttgatcgcct cggcgagccc
     2041 gttggagatc tcacgctccg gcagtgtggc gacccagtcc aggtctgccg ccaccaggcg
     2101 gggccttacg aaggcgccga ccaggttctt gccttcgggg aggtcaatgc cgactttgcc
     2161 gccgacggcg ctgtccacca tggcgagaag ggaagtcggc aacatgacga ggtctatgcc
     2221 ccgcatgtag gtgcccgcga cgaagcccgc cagatccccg accaccccgc caccgagcgc
     2281 gaagatggtg ccgtcacggg tcaatcccat gcgggccagg cgcccgaggg agtctgcgta
     2341 aacgtccagg ctcttggagg actcgccggc gggaagctcg atcttcccag cgacgcgcca
     2401 gccggccgac tgaagggcat tccgcacccg cgcggcgtgc aaaggcccca ccgtcgagtc
     2461 cgtgaagatc gcgcatcctc cgggctcgag cgaaccggag aagatctccg cgaggtcgat
     2521 tccggcgccg atgaagaccc tgtacggttg ggtgacgggg accgctaccg tctcgacagg
     2581 cacctcagca cctcctccgc caccttcgcc ggagagcgtc catcggcctc gacctcgaga
     2641 tcggcgacct ccctgtagag agggagacgt tctgcgtagc gcagcccgaa ctcttcgcgg
     2701 ctcgccgccc gaagggggcg gccagcacca cgagtcc
//
LOCUS       DATMVF010000231         6081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_131027, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000231 DATMVF010000000
VERSION     DATMVF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131027"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..960
                     /locus_tag="VN178_11910"
     CDS             421..960
                     /locus_tag="VN178_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81719.1"
                     /translation="MADPVEACLHTEGLRVTSGSEYCCPVCEECLDLDGVVRWVQEAE
                     KSRDEAYSIVEGDGFGEIVRQELQREVYRRRRIMYELENVPRVVTARPEMILVIHDAV
                     EASYECRIFYKEPRPAGVVEQVSVGALLDAILELRSDPDPIVRLISGKIEEFHVFRSK
                     LTEGGEPAPERRVFYANEL"
     gene            complement(1132..1668)
                     /gene="thpR"
                     /locus_tag="VN178_11915"
     CDS             complement(1132..1668)
                     /gene="thpR"
                     /locus_tag="VN178_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWS81720.1"
                     /translation="MRTFVAVFPPPEVREALFRAARDLPASNAFRLIGQKKLHLTLKF
                     LGNVAVEDLSGVEQALERLRGRHEPFEVETSGFGAFPSGRKARILWAGVGEGSGQLRV
                     VARSVDDLLEPVGFGREKRPYVPHLTLGRSRGRGAKLEGANVSPPKLRFTVSGVDLVE
                     SVPERSGVAYSVLETYSF"
     gene            complement(1676..2455)
                     /gene="larB"
                     /locus_tag="VN178_11920"
     CDS             complement(1676..2455)
                     /gene="larB"
                     /locus_tag="VN178_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWS81721.1"
                     /translation="MDELENTLAAVASGELSPESAAGRLKRGEVRYLDEFAALDLGRS
                     IRKGVPEVVYASGKSPSQVASICATLLESCERVIVSTPTPEHEATIRAALPETQLRWA
                     GRSLVIGSGEPAPSGGRVGALSAGTSDLPVLEEALAVAGEMGASVMGFHDVGVAGVHR
                     LAGPLQELRAFDPDCLVVAAGMEGALPSVVSGLVAVPVIGLPTSTGYGLGGDGTAAIL
                     GMLQTCSPGLCVVNVDNGVGAGATAALIANRAAAGRKSSPD"
     gene            2538..3557
                     /locus_tag="VN178_11925"
     CDS             2538..3557
                     /locus_tag="VN178_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81722.1"
                     /translation="MTIIELVLSLAAILGAAILFTNAVEIMGDRLNLGAGAVGSILAA
                     VGTALPETMIPLVAILGSVLLGSSGAAAGEIGIGAILGAPFLLATLAVFIVGAAALGY
                     KGRRETGSEITADRETAIPDLGFFLVCFVPAAAAGLLPLPLVLKIVLAVALLAAYVLY
                     VVRTVRKGGEAEGDVPARLTLWPFRSQGPTWAVAAQLIGTIAVMAGGAHLFVGAVEHL
                     SKSVGIPAGLIALVLAPLATELPEKFNSVIWLRDNKDTLAIGNITGAMVFQSTIPVSV
                     GVLFTTWRLDFLSAVAAIFAILSSIVFLGFLLRKAPLRAFYMLGAGSLYIVFVVTAII
                     YIVRG"
     gene            3644..4345
                     /locus_tag="VN178_11930"
     CDS             3644..4345
                     /locus_tag="VN178_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565941.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HWS81723.1"
                     /translation="MEFMTGEWFGRLLGVLVVNLILSGDNAVVIAMAARTLEGANRRR
                     AIIWGAAGAVILRLLFAGIITLLLGIPLLQVVGGLLLVWIAWKLIHEGEGEGDEEKVK
                     AGTSTWQAVRIIIVADAVMSLDNVIALVQAARIGGEVDITLLAIGLATTIPLVIFGAA
                     LLTSLLNRFPFLVYLGAGLLIYLAVEMIFADKVTHPYLKHYHSIEWIIGLGAAVIFMV
                     VAWLWARRSSGEETG"
     gene            complement(4356..4781)
                     /locus_tag="VN178_11935"
     CDS             complement(4356..4781)
                     /locus_tag="VN178_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81724.1"
                     /translation="MPEPSVSVSEAARFLDVPEEEARELADPEGNVPLETLRSRAGAA
                     TIARELAGAREEVGRLSGQLREVRASNVRLQKELRDSVEERRRLTDEVLDLRAAAEER
                     LMLMERIEHISRVEQELEESSSELERLRSRRLLGRLLNR"
     gene            complement(4785..5021)
                     /locus_tag="VN178_11940"
     CDS             complement(4785..5021)
                     /locus_tag="VN178_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWS81725.1"
                     /translation="MPDVTFVVSDVASAEESEKLERALARLEFVELVNVDSERGLVAV
                     SYEGGEEELGGIEGAIREAGHDAEPSPGADPDVD"
     gene            complement(5259..>6081)
                     /locus_tag="VN178_11945"
     CDS             complement(5259..>6081)
                     /locus_tag="VN178_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWS81726.1"
                     /translation="LGLEGDSATAITNSHVVRGLRSRRAGNPGGEIQVTLSDGSSVPA
                     EVLGDDPPSDLAVVRFDAEEEPTVATLGDAGDLVVGQLVVAIGNPLGFQRSITAGVVS
                     ALGRSIRGESGRLIENVIQTDAAINPGNSGGPLADASARVVGINTAIIGGAQGIGFAV
                     PVSGAFRRVVFTLVTEGRVRRAYLGVVVESRQARGEFEGGAGVRSIAPNSPAERAGLK
                     PGDVIIGLKGETVRSNDDLLNLLDASSIGQDATLRVLRGNKELSLTVRPREQPAE"
BASE COUNT         1023 a         1954 c         1950 g         1154 t
ORIGIN      
        1 agaccggcga ccgtaaccat cccgcgcgcc ccttgccggc cgacgggttt agtatcagat
       61 gtgcctctcc caaggaggag aacatagccg gaaaatacca gaacctaaat ccagcggccc
      121 ggccgagggg ttattcaagc tctactcgag atgcagattg ctacgaaaga cggcccgcaa
      181 cggccggttc acaggagccg cccaaggacg gagtccgcag agacgggaca acggtattag
      241 ggactacttt gcaggtgaaa tagggacccg gcctctcccg aaacactacc gggggccatc
      301 cgaaggtccc tgccgctata gacttccgtt taacatagag ttaaagtcaa gctacagagg
      361 gacttcgtgc tcgttgtgag agtcgcgcag ggttgggaaa ggagtacaat actgtcccac
      421 atggctgacc cggtcgaagc ttgcctgcac acggagggcc tgagggttac ctccggatcc
      481 gaatactgct gtccggtatg tgaagagtgc ctggatctcg acggcgtcgt gcggtgggtt
      541 caggaggccg agaagagccg ggatgaggct tactctattg tcgaaggtga cgggttcgga
      601 gagatagtac gccaggagtt gcagcgggaa gtctaccggc gacggaggat aatgtacgag
      661 ctagagaacg tgccccgggt ggtgacggcg aggccggaga tgatccttgt aattcacgat
      721 gccgtcgagg cttcttatga gtgccgaatc ttctacaagg aaccgcgccc ggctggtgtt
      781 gtggagcagg tgagcgttgg ggcgttactc gacgcaattc tggaacttcg ctccgacccc
      841 gaccctatcg taaggttgat ctccgggaag atcgaggagt ttcacgtctt ccgctcgaag
      901 ctcaccgaag gcggggagcc cgcgccggag cgcagggttt tctacgcgaa cgagctctga
      961 aacctctgag gcgctggcgc acacgttcgg caacggctag aattgaccgc cagatggata
     1021 cctatctgag aagcgcggtc gcatgagcgg gagtaccttt attgcccttg tggtgatgat
     1081 cctgatcctg ctcttcatca tcatgtccat cgggcctctg gttagcgcct tctagaagga
     1141 gtaggtttcc agaacggagt aggcgactcc gcttctctct ggcacgctct ccaccagatc
     1201 cacgccggat accgtaaagc gaagcttggg cggagagacg tttgcgccct cgagcttcgc
     1261 ccctctgccc cgcgaccggc cgagagtgag gtggggtacg tagggccgct tctctctccc
     1321 gaatccgacg ggctccagga gatcgtctac gctccgggcc accacgcgaa gctgcccgga
     1381 tccttctccg acgccggccc agaggatacg ggccttcctc ccagagggga aggccccgaa
     1441 gcccgaggtc tcgacctcga acggttcgtg cctgccgcgt agccgctcga gcgcctgctc
     1501 cacaccgctc aggtcctcca cggccacatt gcccaggaac ttcaaggtca ggtggagttt
     1561 cttctggccg atgagacgga aagcattgct cgccggcagg tcgcgtgccg cgcgaaacaa
     1621 cgcttccctc acctcgggcg gcgggaatac ggcgacgaag gttcgcactg ctatcctagt
     1681 cggggctgga cttgcggcct gcggcggcgc ggttggcgat gagggccgcg gtggcccctg
     1741 cgccgacgcc attatccacg ttgacgacgc agagaccggg ggagcaggtc tggagcatgc
     1801 cgaggatggc tgcggtcccg tcgccgccga ggccgtaccc ggtggaggtg gggagtccta
     1861 tcacgggcac ggcgacgagg ccggagacca cgctcgggag ggcgccttcc atcccggcgg
     1921 cgaccaccag gcagtcggga tcgaacgctc tcagctcctg caaagggccg gcgaggcgat
     1981 ggacgccggc taccccgacg tcgtggaagc ccatcacgga tgcacccatc tcgccggcca
     2041 cggcgagcgc ctcctcgagg acgggcaggt ccgaggtgcc cgcgctcagg gctcccacgc
     2101 ggcctccgct cggcgccggc tcgccgctac cgatcaccag tgagcgcccg gcccaccgca
     2161 gttgggtctc gggaagcgcc gcgcggatag tggcctcatg ctcgggggtg ggggtgctta
     2221 cgatcacacg ctcgcacgac tccagcagcg tcgcacagat gctcgccacc tgggaggggc
     2281 tcttgccgct agcgtagacg acctcgggga cgcccttcct gatagagcgg ccgaggtcga
     2341 gggcggcgaa ctcgtcgagg tagcggacct cgcccctctt caggcgtccg gcggccgatt
     2401 cgggagagag ctcgccgctc gcgactgctg ccagtgtatt ttcgagttcg tccatgcccc
     2461 cgcttccggg tgtagtaagc tcgccagcgg gtaagaatct agcacgttcc tactcccagt
     2521 ggtcgtaagg agagcacttg acgataatcg agttggtcct atccctggcg gcgatactcg
     2581 gcgccgcgat ccttttcacc aatgccgtag agattatggg tgaccggctg aacctcgggg
     2641 cgggggccgt agggagcata ctcgcggccg tcggaaccgc gttgcccgag accatgatcc
     2701 cgctggtcgc gatcctagga agcgtactct tgggctcgag cggcgcggcg gcgggcgaga
     2761 taggtatcgg cgcgatcctt ggcgcgcctt tcctgctcgc gacgctggcg gtgttcatcg
     2821 tcggggcggc ggccctgggt tacaagggcc ggagggagac ggggagcgag atcacggccg
     2881 atagggagac cgccatcccc gacctggggt tcttcctggt ctgctttgtc ccggccgccg
     2941 ccgcgggcct cttacccctg cctctggtgc tgaagatcgt gctcgctgtg gccctgctgg
     3001 ccgcttacgt cctctacgtg gtaaggacgg taagaaaggg cggggaggcc gaaggggatg
     3061 tccccgccag gctcacgctc tggcccttcc gctctcaggg accgacctgg gccgtcgccg
     3121 cgcagctgat cgggacgatc gccgtaatgg ccggcggcgc ccacctcttc gtcggggccg
     3181 tggagcacct ctccaagtcc gtaggtatcc cggcgggctt gatcgcactg gtcctcgcgc
     3241 ccctggcgac ggagctgccg gagaagttca actccgtcat ctggctcagg gataacaaag
     3301 ataccctggc catcggcaat attacagggg cgatggtctt ccagagcacc atccccgtct
     3361 cggtcggggt gctctttacc acctggcggc tcgacttcct cagcgccgtc gcggccatct
     3421 tcgccatcct ctcgagcata gtcttcctcg ggttcctgct gcgcaaggca cccttgaggg
     3481 cgttctacat gctcggcgcc ggttcactct acatcgtctt cgtcgtgacg gcgataatct
     3541 acatcgttcg cggctgaaaa tcgggttggg cctcgtacct gttttccctc tttcctgata
     3601 agatacgtag cctgtcgtat ggtcgaggag gaagccggat cagatggaat tcatgacggg
     3661 agagtggttc ggccgactcc ttggtgtgct ggtcgtcaac ctgatcctct ccggtgacaa
     3721 cgccgtggtc atcgcgatgg cggcccgcac cctcgaaggg gccaacaggc ggagggccat
     3781 catctggggt gcggctgggg ccgtcattct cagactgctc ttcgccggca tcatcacctt
     3841 gctgctcggg attcctttac tacaggtggt ggggggattg ctgctcgtct ggatcgcctg
     3901 gaagctgatc cacgagggtg agggtgaagg cgacgaggag aaggtaaagg ccggtacgag
     3961 tacctggcag gcggtgagga taataatcgt ggccgacgcg gtgatgtcgc tggacaacgt
     4021 gatcgcgctg gtccaggccg ccaggatcgg cggcgaggtc gacatcacgc tactcgccat
     4081 cgggctggcg acgacgatac cgctcgtgat cttcggggcc gcgctgctca cttcgctgct
     4141 gaaccggttt cctttcctcg tctacctggg cgccggtctg ctcatctacc tggccgtgga
     4201 gatgatcttc gccgacaaag taacccaccc ctatctgaag cattaccaca gcatcgagtg
     4261 gatcatcggc ctcggcgcag ccgtcatctt tatggtcgtg gcctggcttt gggcgcgaag
     4321 atcgagcggc gaggagacag gatagggggg ccgctctagc ggttcaggag cctacccaga
     4381 agcctcctgc tcctgaggcg ctcaagttcg gaggagctct cctccagctc ctgctcgact
     4441 cgggatatgt gctcgatacg ctccatgagc ataagcctct cctctgccgc cgcccgcaga
     4501 tccaacacct cgtccgtcag gcgcctgcgc tcctctaccg agtctctaag ctctttctga
     4561 agacgtacgt tggaagcgcg aacctcccta agctggcccg ataaccttcc gacctcctcg
     4621 cgggcgccgg ccagttccct cgctatcgtc gccgccccgg cccggctcct caaggtctcc
     4681 aggggaacat taccctcggg gtcggcgagc tctctcgcct cctcttccgg aacgtccaga
     4741 aagcgggccg cttctgagac agagacggag ggttcgggca tggatcagtc cacgtcaggg
     4801 tcggcgccgg gggaaggttc ggcgtcgtgg cccgcctccc ttatggcacc ctcgataccc
     4861 ccgagctcct cctccccacc ctcgtaggag acggcgacga gccccctctc cgagtccacg
     4921 ttcacgagtt ccacgaactc caggcgcgca agagcccgct ccagcttctc ggactcctca
     4981 gcgctcgcta cgtcgctaac cacgaaagtc acgtcgggca tggtcgctcc cttcggtcgc
     5041 tcagcaaatc agcacgctca ggcttttagc cttcgcttgt cagcacttca gcttgtcagc
     5101 tttctgtcca agcggcgaga ccaccactcg gctacgccgc cctgtcgaga acgtaagttt
     5161 ggtccatctt tgtccggttg tcatcctccc tgttcgccga ccacgttctt gctgaaatgc
     5221 tgactagctg acaagccgcg aagcggcgtg ctgaaatgct attccgctgg ctgttctctg
     5281 ggccgcacgg tgagcgagag ctccttgttg ccccgtagca cccgcagcgt ggcgtcctgg
     5341 cctatggacg atgcgtcgag caggttgagg agatcgtcgt tcgatcggac ggtctctccc
     5401 ttgaggccga tgatgacgtc tcccggtttc aagccggccc gctctgccgg gctgttcggg
     5461 gcgatgctcc tcacgccggc gccgccctcg aactctccgc gtgcctggcg gctctccacg
     5521 accaccccga ggtaggcgcg gcgcacccgc ccctccgtta cgagcgtgaa gaccacgcgg
     5581 cggaacgcgc cggagacggg gacggcgaag ccgataccct gcgcgccgcc gatgatggcc
     5641 gtgttgatgc cgacgactcg tgcggaggcg tcggccaggg ggccgcccga gttgcccggg
     5701 ttgatcgccg cgtcggtctg gatcacgttc tctatgagcc ggccgctctc gcccctgata
     5761 gaacgcccga gggcgctcac caccccggcc gtgatactgc gctggaagcc taaggggttc
     5821 ccgatcgcga cgacgagctg gccgactacg agatcccccg cgtcgcccag cgtggcgacg
     5881 gtcggctcct cctcggcgtc gaagcggacg acggcgagat cgctcggcgg atcgtcgccg
     5941 agcacctctg ccggaaccga tgacccgtcc gagagcgtga cctgtatttc tccgcccggg
     6001 ttgccggccc tgcggctccg caggccgcgc acgacgtggc tgttggttat ggcggtggcc
     6061 gagtcgcctt cgaggccgag t
//
LOCUS       DATMVF010000232        12611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_442088, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000232 DATMVF010000000
VERSION     DATMVF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12611
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442088"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2013)
                     /gene="pknB"
                     /locus_tag="VN178_11950"
     CDS             complement(<1..2013)
                     /gene="pknB"
                     /locus_tag="VN178_11950"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HWS81727.1"
                     /translation="MQQLVDNRYRLLKSLGSGGMADVYLAHDDILDRDVALKVMSSRY
                     ASDEEFVERFKREAQSAAALSHPNIVSIFDRGETEDGTYYIAMEYLPGGTLKDRIIKR
                     GALPARTAAAVALQMAEALRAAHERDVIHRDIKPHNILITELGDVKVTDFGIARAAAS
                     STMTRTGHILGTAHYISPEQAMGEPVGPASDLYSLGIVLYEMLTGELPFDADTPLGIA
                     MKHVNGHLRQPKELNPTVPDGINAITIRLLAKNPEDRYTSDSELIDDLERVCAGLDPT
                     AATTEMMTHAAAATTRLTPPPPPQRVGRNKMRRRRVAPLLLALLALLVVAWAGYSLLQ
                     ERLVGIAGPQIGVPDLVGMSLDGAREKVGDDFQVEVAKSVSGESPVDTIISQSPSAGK
                     AEKGSTISVSIVGTRVSDVPDVVGSGRQEAEQALEDAGFQVEVAERASSFENQDRVMS
                     QNPSGGTRAEVGSGVTITVGTGPSSVPVPRLYGRTPAQAEAVLQEAGLSLGTVTRDYN
                     NDVAQGSIFFQVPSGGANVEPGTVVNATVSLGPEQVEVPEVYGLSVAAAQTALSNAGL
                     NSTPIEVEGDEEAGTALSTDPGVGAMLDPGSTVSLYYSAGPPPATTASPDTSSAPDAP
                     SNPERSQDSDRRARDERNTEAQPADENRPQRNEPTTPDNSGSGNSGQ"
     gene            complement(2049..3497)
                     /locus_tag="VN178_11955"
     CDS             complement(2049..3497)
                     /locus_tag="VN178_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWS81728.1"
                     /translation="MGGSVNAHLRRTFYLFAAGFAGLVMVLVYWQVYSREALANNPEN
                     GFQTQRSVSSPRGLILAGDGETVLAKSVKRPTPTGPVYDRVYPLGEPFANVVGYWSTR
                     YGATGIEIGNNSDLSAIVGEPETLDDLINRATGGPQPGNNVILTLDPELQELAYQELS
                     SSSTGRGAVVALNPKTGEILAMATAPSFDPNQVNENYPELASAPNAPLVDRAAQSLYP
                     PGSIFKVITAAAALEAGVSPQQRFFDSGAYVLPGYTVENFEGKDYGEVTFTRALAYSI
                     NVVFAKIAVEIVGAEALTQMALNFGFGDPYDDFPLPVSGSSVNSLPPEQWTTGTLAQT
                     AFGQGEAQTNAFEMAVVAGAVANDGKIMEPRIVREVRSPDGIILNKPTPSVRRQALPP
                     ATADTLNVMMQEVVIQGGFTEAQIPGMKVAGKTGTAESGNGPPHSWWITFAPADDPEI
                     AMCVMIENGGRADRSAMPLAASLMKAYLEDGI"
     gene            complement(3482..4795)
                     /locus_tag="VN178_11960"
     CDS             complement(3482..4795)
                     /locus_tag="VN178_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWS81729.1"
                     /translation="MTQRAFTPMILALLVTTLGFATLIFVQEAASPPLAYGAYYAGTL
                     FVLYLGVRLLLPHSDVLLVPIVTLLTGLGLVMIFRLTYNVEGSKELALTQAVWILIGS
                     GAMFLVVLFFRNYDRLFDYKYLLAIGAVFLIASTFTPLGYSVNGARLWVDLGPVNFQP
                     SEFARIALVIFFAGYLAEKRDVMAATSRTFLGIQLPAIKYFGPVALVWAASLGLLVFE
                     KDLGSSLLFFAVPLLMLYAATGRTAYVLLGGVLFSLGSFVAFLLFEHVQLRVQSWLDP
                     WRDPDGTGFQILQSIFNIADGGLLGTGLGAGFAQTIPEVETDFVFSAIASELGLLGAT
                     AVLLAFLAFTYRGIKIALLAEDDASKLLAYGLTAMFAIQTLIIVGGVTKAIPLTGITL
                     PFVSYGGSSVVGNFILTGLLLLVSERAGRREAEGLVYRDDPWGAP"
     gene            complement(4797..5969)
                     /locus_tag="VN178_11965"
     CDS             complement(4797..5969)
                     /locus_tag="VN178_11965"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWS81730.1"
                     /translation="MPFLELLPFGLTHAGKVRQNNEDALLVGGGEDETLFVVADGIGG
                     FEAGEVASSLAIDVLKDLQPDQPFKAAIGEANRRIVAAGRGDEKLSGMGTTVVAIRFG
                     GKQGEPVAEVAHVGDSRAYLVRGGDMNPITEDHSLVAELVRSGDLTRDEAAEHPQKNL
                     ITRALGADEEVDVDTAIVPIEAGDRILLCSDGLSDMVSETGISEILAEFPDDPERAAR
                     VLLSAALDAGGNDNITVVVVDVKEQPAADVRESRSGASSEMLHSGQGVEAPSTPPRAR
                     PRRSTRAARRKRSRGLRRGLGKLVRGLAIVLVLVLALTPAYLWGSSRYFFEFQEGEVV
                     AYQGLPYAPFGIELHQEWRRPGLKESQIKEPYQKPIENRKLYTRDQAERVLEDLGS"
     gene            complement(5974..6459)
                     /locus_tag="VN178_11970"
     CDS             complement(5974..6459)
                     /locus_tag="VN178_11970"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWS81731.1"
                     /translation="MLELQVPLLAAKALILLLLFAFIYAVMSRSIGDLRKIRDDEPYV
                     PGRGSNGRGTYARRPSAPGVPGLVVEDSEILAPETRFELGDGETSMGRSSGSTIVLRS
                     DDYASGRHAQLTRHGGLLYVEDLGSTNGTFVNGRKTVGATPLRNGDTVRVGSTTFRYE
                     E"
     gene            complement(6463..7290)
                     /locus_tag="VN178_11975"
     CDS             complement(6463..7290)
                     /locus_tag="VN178_11975"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HWS81732.1"
                     /translation="MGFLKHIEKRMESLVEGVFGRAFRRQIHPVEIAKGLTKQMDEGR
                     MVSISRTYAPNDFTVHLSREDSESIRAYQASLNDELIQYASTHAENKNYHLMTPPRIR
                     FETEDTLRFGEFGVTAKLTGGDGPREKGAPQDTSGQTRIFRTEESSGGQMDQGTATIS
                     ADEARRHGLAREIVEIVLGDEKHPLEGRGPWSVGRSQENDIVINDPNVSRRHARISRS
                     DGGFVVEDLGSTNGTLLDGAPIDRERIEGGDELTFGQSTARFIRRIDSPGEEWGGRR"
     gene            complement(7297..8925)
                     /locus_tag="VN178_11980"
     CDS             complement(7297..8925)
                     /locus_tag="VN178_11980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWS81733.1"
                     /translation="MTETRYIEGQGRYALAEMVGRGGFATVWRARSLKSSSRGKDVAV
                     KVIPVYNAGERSRALREGQIAEGLRHENIVETLEVIPGDHEIYLVTEYVRGMPLDEAA
                     KGYGIEGIVDSLSQILEALVYAHGQGIIHRDIKPQNALVDARGRVKLTDFGVAFRAGD
                     TRLTRVGFAVGTPGYIAPEILDGADPTALTDIYAVGATARTLLASQPYEPPPRLREFV
                     DRTTSPNPDHRPQSAWAAVKLLTGRKAPSGSISSGGGRVPDRLSGGFKDGALRTINGL
                     AAGCLGYLLAAEILADSAQAAGVAAGLGVLAYLLPRLGALGVIIALAVLLIRNSAGLG
                     FTALVPVLGGVWVLGAGAANADVRRLPLGPALAVPLALLVGLGTLVGSALLAGLPLLF
                     GALMRPLGACLSAAAGALTLICYDLTVRNGFFADGPYAELPFSGEKLDVLPITLGVGG
                     LLDYSQGYLNYFPKLPLLIPLWAVVAGIVSLAEWTGQWMVGLAVAVVVGGLGYALLIS
                     PNAPPGTLTSNMISLGLAAIIYGVLRYLVSRVRG"
     gene            9240..9323
                     /locus_tag="VN178_11985"
     tRNA            9240..9323
                     /locus_tag="VN178_11985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9274..9276,aa:Leu,seq:cag)
     gene            9335..10426
                     /locus_tag="VN178_11990"
     CDS             9335..10426
                     /locus_tag="VN178_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81734.1"
                     /translation="MGVCIERRSHRAAPAARRQGASGRPGRTRSSPKERRRRTLPAWL
                     TLAGSILLLVVVFGFIFFVARGCVATQQSTQVRKYVTSADSFLSDSSNAGREELQRIL
                     GNAGGNPARLDDEALAQAANRTEQQYLLALRQEEIPPEFEDDHHYIITALGIRFQATD
                     KLVQAASGDASGFAGELAMAVEDYRTSDSIVANYYIPAVKRSLESAGQTSDQTYLEEP
                     QSFMDYGALGFDTAPVSGTARSDPNALHGVRVTAVRVAGKPLNSGGNVVLTGADEPTF
                     AVTVGNDGEVAETGVEVEVVLNTRAERQAESKTLARLEPDKMATVEVGGFIPGELDES
                     ARVTVEAGPVEYEKRTANNTLTGRVTFGI"
     gene            10580..10858
                     /locus_tag="VN178_11995"
     CDS             10580..10858
                     /locus_tag="VN178_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWS81735.1"
                     /translation="MVTAIVLVRTERERVQEAAQAMLAIDGVTEAYSVTGPYDLVVMV
                     RIPDFESLADVVPERIAQVPGVARTETMVAFRAYSNYDLDRAWSLGFE"
     gene            complement(11120..12247)
                     /locus_tag="VN178_12000"
     CDS             complement(11120..12247)
                     /locus_tag="VN178_12000"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWS81736.1"
                     /translation="MPIEFKEGASDYSLGVEEELPIVDATTGELVQKIEEIMSRLPAE
                     LHDSVSYELFQSVMEIKTPPCDSTSEVEKHLRELRSRVGSWAAACGASLASAGTHPFS
                     RYRDQKVTNQERYKNVLRTLRWIAQREVIFGQHVHVAVPGPEEAIVAHNRLAEQTPIL
                     LALSANSPFWQGFDTGFESARVKIFESFPRAGLPPAFPDYGSFEGYVDLMVASGTMED
                     YTFCWWDVRPHPKIGTIELRVLDSQTNLKDTLALTALTQCLVAEVLEEVGPHEPYNMH
                     LAVENKFRAARYGMDAAFYDARDDITVPARDLARNLVERLKPHAQDLGCASELEGVLD
                     IVEGGTGSQRQREVYKESGNFLDVVAFLIEGTRPALAEEQS"
BASE COUNT         2145 a         4046 c         4191 g         2229 t
ORIGIN      
        1 ctggccactg ttgcctgacc cgctgttatc cggggtggtg ggctcgttgc gttgcggccg
       61 gttttcgtcg gcgggttgcg cttctgtgtt ccgctcgtca cgggcccgcc gatcggagtc
      121 ctgcgatcgt tcgggattgg acggagcgtc gggcgccgat gaggtatcgg gggaagccgt
      181 tgtagcggga gggggacccg cgctgtagta gaggctaacg gtagagcctg gatcgagcat
      241 cgcgccgacc cctgggtcgg tcgagagcgc ggtgcctgcc tcctcgtcac cctcgacctc
      301 gatgggggtg gagttcagcc cggcgttgga gagcgccgtc tgtgccgccg ccacgctcag
      361 gccgtagacc tcagggacct cgacctgctc cggacccagg ctgacggtgg cgttgaccac
      421 ggttccgggc tccacgttcg ctcctcctga ggggacctga aagaagatac tgccctgtgc
      481 cacgtcattg ttgtagtccc gggtcacggt gcccaaactc aggcccgcct cctgcagtac
      541 agcctcagcc tgcgccgggg ttctcccgta cagccgcgga acaggcacgc tcgaagggcc
      601 ggtccccacg gtgatagtca cgcccgatcc cacctcggca cgcgtaccgc ccgatggatt
      661 ctggctcatg acccggtcct ggttctcgaa ggagctcgcc cgctcggcga cctcgacttg
      721 gaaacctgcg tcctccagcg cctgctccgc ctcctggcgt cccgagccca cgacgtcggg
      781 cacatccgac acccgcgtcc cgactatgct gaccgagatc gtcgaacctt tctccgcctt
      841 tccggcgctg ggactctggc tgattatggt gtcgacggga ctctcgccgg agacgctctt
      901 tgcgacctcg acctggaaat cgtcacccac cttctctctg gcgccgtcca ggctcatgcc
      961 gaccaggtct gggacgccaa tctgcggccc tgcaatgccg accagccgct cctgaagaag
     1021 ggagtacccg gcccaggcaa cgaccaggag cgcgaggagg gctagcagca gcggcgcaac
     1081 cctcctacgc cgcatcttgt tgcggccgac tctttgcggg ggaggtgggg gggtgaggcg
     1141 cgtggtcgcc gcagcggcgt gggtcatcat ctccgtcgtc gcggcggtgg ggtcgaggcc
     1201 ggcacagacg cgctcgaggt catctatgag ctcggagtcg ctcgtgtagc ggtcctcggg
     1261 gttcttggcg agcagcctga tcgttatggc gtttatgccg tcgggcaccg tggggttgag
     1321 ttccttcggc tgccgcagat gcccgttgac gtgcttcatc gcgatcccca agggtgtgtc
     1381 ggcgtcgaag gggagttcgc ccgtgagcat ctcgtagagg acgatgccca aagagtagag
     1441 gtcgctcgcc gggccgaccg gctcgcccat ggcctgctcg ggcgagatgt agtgggccgt
     1501 accaaggatg tgacccgtcc tcgtcatggt cgaagatgcc gccgcgcggg ctataccgaa
     1561 gtccgtgacc tttacgtccc cgagttcagt gatcaggatg ttgtggggct ttatgtcgcg
     1621 gtggatgacg tcccgctcgt gcgcggccct caaggcctcc gccatctgga gcgccacggc
     1681 cgccgccgtg cgggcgggga gcgcgccgcg cttgatgatg cggtccttca gggtgccacc
     1741 gggcagatac tccatcgcga tgtagtaggt cccgtcctcg gtctcgcccc tgtcgaagat
     1801 agagacgatg ttggggtgcg agagggctgc ggcgctctgg gcctcacgct tgaagcgctc
     1861 gacgaactcc tcgtcgctgg cgtagcggct gctcatcacc ttgagggcca cgtccctgtc
     1921 gaggatgtcg tcgtgtgcca ggtaaacgtc ggccatgcca ccgctgccca gtgacttcag
     1981 aaggcggtaa cgattgtcta cgagctgctg cacggacccc agattataga cgcgagcgcg
     2041 gacaatgttc agattccgtc ctcgaggtag gctttcatca ggctggccgc caggggcatg
     2101 gcgcttctat cggccctccc gccgttctcg atcatcacgc acatggcgat ctccggatcg
     2161 tcggcggggg cgaaggtgat ccaccacgaa tgcggggggc cgttgcccga ctcggcggtt
     2221 cccgtcttcc ccgctacctt catgccgggt atctgggcct cggtgaaccc cccctggatg
     2281 acgacctcct gcatcatcac gttgagggta tcggccgtag caggtgggag cgcctgccgg
     2341 cggacgctgg gggtcggttt gttcaggatg atgccgtcgg gggagcggac ctcgcgcacg
     2401 atgcggggct ccatgatctt gccgtcgttg gcgacggctc ctgcgacgac agccatctcg
     2461 aaggcgttgg tctgcgcctc accctggccg aaagccgtct gggcgagggt gcccgtggtc
     2521 cactgctcgg gcgggaggct gtttacgctg ctccccgaga cgggtaacgg gaagtcatcg
     2581 taggggtccc caaagccgaa gttcagggcc atctgcgtga gggcctcggc ccccactatc
     2641 tcgaccgcga tcttggcgaa gaccacgttg atggagtagg cgagcgcccg cgtgaacgtc
     2701 acctccccgt agtccttgcc ctcgaagttt tctacggtgt agccgggcag cacgtaggcc
     2761 ccgctgtcga agaatctctg ctgcgggctg accccggcct caagcgccgc ggctgccgta
     2821 atcactttga agatcgaacc gggtgggtag aggctctggg ccgcgcggtc gacgaggggc
     2881 gcattgggtg cgctggcgag ctcggggtag ttctcgttta cctggttggg gtcgaaggac
     2941 ggggctgtgg ccatcgcgag gatctccccg gtcttgggat tcaaagcgac cacggcaccc
     3001 ctgcccgtgg agctcgacga gagctcctgg tatgcgagct cctgaagctc ggggtcgagg
     3061 gtgaggatca cgttgttgcc gggttgcgga cctcccgtgg cgcggttgat caggtcgtcc
     3121 agggtctccg gttcccccac tatggcggag aggtccgagt tgttgccgat ctctatgccc
     3181 gtcgccccgt agcgggtgct ccagtagccg accacgtttg cgaacggctc gcccagggga
     3241 tagacgcggt cgtagacggg accggtcggg gtgggacgct tgacgctctt ggccagcacg
     3301 gtctcgccgt cgccggccag gatcaaaccc cgcggcgagg agacgctgcg ctgggtctga
     3361 aaaccgttct ccgggttgtt ggccagcgcc tccctggagt agacctgcca gtagacgagc
     3421 accattacga gaccggcgaa cccagccgcg aacaggtaga aggtacgcct gaggtgggcg
     3481 ttcacggagc cccccacgga tcgtccctgt agacgagccc ctcggcctcg cgccgtcccg
     3541 cccgctccga gacaagcagg aggaggcccg tgagtatgaa gttgccgacg acggaggagc
     3601 ctccgtaaga gacgaagggc agcgtgatcc ccgttagcgg aatggccttg gttaccccgc
     3661 ccacgataat cagggtctgg atggcgaaca tcgccgtgag cccgtaggcc aggagcttgg
     3721 acgcgtcatc ctcagcgagc agggcgatct tgatccccct gtaggtgaag gccaaaaagg
     3781 cgagcaagac cgccgtcgcc ccgagcaggc cgagctcgct ggcgatcgcg gagaagacga
     3841 agtcggtctc gacctcgggg atggtctggg cgaagcctgc cccgagcccc gtaccgagca
     3901 gcccgccgtc cgcgatgttg aagatgctct ggaggatctg gaaccccgtc ccgtcggggt
     3961 ccctccacgg gtcgagccag ctctgcaccc tgagctgcac gtgttcgaag aggaggaagg
     4021 ccacaaagga gcccaaagag aagaggactc ccccgagcag cacgtaggcg gtgcggcccg
     4081 tcgcggcgta gagcatcagc aggggaacgg cgaagaagag caggctcgat ccgaggtcct
     4141 tctcgaagac gaggaggccg agggaggcgg cccagacgag ggccacgggg ccgaagtact
     4201 ttatcgcggg gagctgaatg ccgagaaagg tgcggctcgt cgcggccatc acgtcgcgct
     4261 tctcggccaa atacccggcg aagaagatca cgagggcgat gcgggcgaac tccgagggct
     4321 ggaagttgac cggcccgagg tcgacccaga gccgcgcccc gttgaccgag taaccaaggg
     4381 gcgtaaacgt cgaggctata aggaagaccg ccccgatggc gagtaggtac ttgtagtcga
     4441 agagcctgtc gtagttgcgg aagaacagca ccaccaagaa catggcgccg ctccctataa
     4501 ggatccagac cgcctgcgtc agggcgagct ccttcgaccc ctccacgttg tacgtcaggc
     4561 ggaagatcat gacgagcccg agccccgtca acagcgtgac tatcggcacg agaagcacgt
     4621 cggagtgggg caagagaagc ctcaccccga ggtagagcac gaagagggtc cccgcgtagt
     4681 aggcgccgta ggcgaggggc gggctcgccg cctcctggac gaagatgagg gtcgcaaaac
     4741 ccaaagtggt cacgagcagt gccaggatca tgggcgtgaa ggcgcgttgg gtcaccctag
     4801 ctacccaggt cctcgaggac ccgctctgcc tggtccctgg tgtagagctt gcggttctct
     4861 atgggcttct ggtagggctc tttgatctga gactccttga ggcccggtct gcgccactcc
     4921 tgatggagct cgatgccgaa aggagcgtag ggcaggccct ggtaggccac aacctctccc
     4981 tcctggaact cgaagaagta tctgctcgac ccccaaagat aggccggcgt cagggctagg
     5041 acgaggacca aaacgatcgc caacccccgc acgagcttcc cgagacccct ccggagacca
     5101 cgagaccgct tccgacgcgc ggccctggta gaccgcctgg gccgtgccct gggtggcgtg
     5161 cttggcgcct cgacaccctg ccctgaatgg agcatctcgc tggaggcgcc ggagcgactc
     5221 tcccgtacat ccgccgcggg ctgctccttt acgtctacga cgacgacggt gatgttatcg
     5281 ttgccgccgg cgtccagggc ggcggagagc aggacgcggg ccgcccgctc ggggtcgtcc
     5341 gggaactcgg ccagaatctc cgagatcccc gtctccgaga ccatgtccga gagcccgtca
     5401 gaacagagca ggatgcggtc gccggcctcg atgggcacta tggcggtgtc gacgtcgacc
     5461 tcctcgtccg ccccgagggc gcgtgtgatg aggttcttct ggggatgctc cgctgcctca
     5521 tccctcgtca ggtcgccgct gcgcacgagt tcggcgacga gcgagtggtc ctccgttatg
     5581 gggttcatgt cgccgccgcg cacgaggtag gccctcgaat caccgacgtg cgccacctcg
     5641 gccacgggct ccccttgctt cccaccgaac cttatggcga ccacggtggt gcccatcccc
     5701 gagagcttct cgtcgccgcg accggcggcc actatccttc ggttcgcctc tcctattgcc
     5761 gctttgaagg gctggtccgg ctggagatcc ttgagcacgt ctatggcgag ggagctggcg
     5821 acctcgccgg cctcgaagcc gcctatcccg tcggcgacga cgaagagcgt ctcgtcctcg
     5881 cctccgccga cgagcagggc gtcctcgttg ttctggcgga ccttgccggc gtgggtgagg
     5941 ccgaatggca gtaactcgag gaaaggcacg gccctactcc tcgtacctga acgtggtcga
     6001 gccgaccctg acggtgtccc cgttcctcaa gggcgtggcg cccacggtct tgcgtccgtt
     6061 cacgaaagtc ccgttcgtcg agccgagatc ctcgacgtag agcaaacccc catgcctggt
     6121 gagctgggcg tggcgacccg aggcgtagtc gtcgctcctg aggacgatgg tgctgccaga
     6181 ggagcgaccc atgctggtct cgccgtcccc gagctcgaac cgcgtctcgg gggcgaggat
     6241 ctccgaatcc tcgaccacca gcccaggaac cccaggggcg gaggggcggc gggcgtacgt
     6301 cccgcgcccg ttgctgcccc gtcccggcac gtagggctca tcgtccctta tcttccgcag
     6361 gtccccgata ctcctgctca tcacggcgta gataaaggcg aagagcaaca gcaagatcag
     6421 ggccttcgcc gcgagcagcg gcacctggag ctcgagcatc ccctaacgtc tcccgcccca
     6481 ctcctcaccc ggagagtcta tgcggcggat gaagcgggcc gtgctctggc cgaaggtcaa
     6541 ctcgtcgccg ccttcgatcc tttcgcggtc gatgggggcg ccgtctagca gcgtgccgtt
     6601 cgtagaaccg aggtcctcca cgacaaagcc gccgtcggag cgggagatcc tggcgtgtct
     6661 tctcgagacg ttcgggtcgt ttatcacgat gtcgttctcc tgcgagcggc ctacgctcca
     6721 cggaccacgc ccttccaacg ggtgcttctc gtccccgaga acgatctcca cgatctcccg
     6781 cgccagaccg tggcgcctgg cctcgtcggc agatatcgtg gccgtgccct ggtccatctg
     6841 cccgccgctc gactcctcgg tacgaaagat gcgggtctgg cccgaggtgt cctgcggggc
     6901 gcccttctcg cggggaccgt cgccgcccgt cagcttggcc gtgacgccga actccccgaa
     6961 acggagcgta tcttcggtct cgaagcgtat cctcggggga gtcatgaggt ggtagttctt
     7021 gttctccgcg tgggtcgagg cgtactggat cagctcgtcg ttcaaagacg cctggtaggc
     7081 cctgatagac tccgaatcct ccctggagag gtgcaccgtg aaatcgttcg gggcgtaggt
     7141 gcgcgagatc gagaccatcc tgccttcatc catctgtttg gtgaggccct tggcgatctc
     7201 tacgggatgg atctgccgcc tgaacgccct tccaaagacg ccctctacca gcgattccat
     7261 ccgcttctcg atgtgcttga gaaaacccaa agaaacctaa cctctcaccc tcgatacgag
     7321 gtacctgagc accccgtata ttatagccgc gagaccgagc gaaatcatat tggaagtgag
     7381 cgtgcctgga ggagcattcg gggaaatgag caaagcatac cccaggcccc ccacgaccac
     7441 cgcgaccgcg aggccgacca tccactgccc ggtccactca gcgagcgaga cgatcccggc
     7501 caccacggcc cacaggggga tcaggagcgg cagtttgggg aagtaattca ggtacccctg
     7561 ggaataatcg agcaggccgc cgactcccag cgtgatcggc aagacgtcga gcttctcccc
     7621 cgagaaaggc agctcggcgt aagggccatc ggcgaagaag ccgttgcgca ccgtgaggtc
     7681 gtagcagatc aaggtaagag ctccagcggc cgccgaaagg cacgctccta gaggacgcat
     7741 cagcgcgccg aagagcagcg gcagtccggc cagcagggcg ctacccacga gcgtccccag
     7801 cccgacgagg agggccaacg gcaccgccag cgccgggcca agcggcaacc ttctcacgtc
     7861 ggcgttggcc gcaccggcgc ccagcaccca gacgcccccg aggacaggca cgagcgcggt
     7921 gaacccgagg ccggcactgt tccgtataag caggacggcg agggcgatta tgaccccgag
     7981 ggcgcccagg cgcggcagca ggtaggcgag cacaccgagc ccggcggcga cgccggcagc
     8041 ctgcgcgctg tccgccagga tctcggccgc gaggagatac ccgagacacc ctgcggccag
     8101 gccgttgatg gtgcgcaaag caccgtcttt gaaaccgccc gagagcctgt cgggcaccct
     8161 gcctccaccg gacgagatgc tgcccgacgg ggccttcctg cccgtaagga gcttgacggc
     8221 ggcccacgcg ctctgcggac ggtggtccgg gttcggcgag gtggtcctgt cgacgaattc
     8281 cctgaggcgc gggggtggct cgtagggctg gctggcgagc aaagtacggg ccgtggcgcc
     8341 gacggcgtag atgtcggtga gcgcggtcgg gtcggcgccg tccaggatct ccggcgcgat
     8401 gtagccgggc gtgccgacgg cgaagcccac gcgcgtgagg cgcgtgtccc ccgccctgaa
     8461 cgcgaccccg aagtccgtca gctttacccg cccgcgggcg tccacgagcg cgttctgcgg
     8521 ctttatgtcg cggtggatta tgccctgccc gtgggcgtag accagggctt cgaggatctg
     8581 ggacaacgag tccacgatcc cttctatgcc gtaccctttg gccgcctcgt cgagcggcat
     8641 cccccgcaca tactccgtta cgaggtagat ctcatgatcg ccggggatga cctcgagggt
     8701 ctccactatg ttctcgtgcc tgagcccttc ggcgatctgg ccctccctta gggcgcgcga
     8761 gcgctcgccg gcgttgtaga cggggatgac cttgacggcc acgtctttgc ccctcgagga
     8821 gctcttgaga gaacgggcgc gccatacggt cgcgaagccg ccgcgcccga ccatctcggc
     8881 cagcgcgtag cgtccctgtc cttcgatgta gcgcgtctct gtcactactt gataggctat
     8941 tgtatagcct atcagccgtc agcacggcgc ttcgcgcctc gtcagcacgc tcagcccttc
     9001 gtgcttcgct tgtcagcagg tcagcttctt ccactgtggc ctcgcttgac agcgtgagag
     9061 aaccgctgct cggggcgggg taggttttac gcttgtgagg tggctccggg aggagtagct
     9121 gacgagccgc cgagcggagc gaggcggcgg gctgacaagc ctgcgaagca ggcgtgctga
     9181 agtgctgaca tgctgataca atcccccgca gttcaggacg tttcccggag agagcctgcg
     9241 ggcgagtggc ggaattggta gacgcgctag gttcagggtc tagtggccga gaggccgtgg
     9301 gggttcgagt cccccctcgc ccacgacagg ttctttgggg gtttgtatag agcgtagatc
     9361 tcacagggcc gcacccgccg ctcgccgtca gggggcgtcg ggtcgacccg ggagaacccg
     9421 cagctctccg aaagagcgcc gccggaggac gctccctgcc tggctaaccc tcgccggctc
     9481 catcctcctc ctggtggtgg ttttcgggtt tatcttcttc gtcgcccgcg gctgcgtggc
     9541 aacccagcag tcgacccagg tccgcaaata cgtcacgagc gcggacagct tcttgagcga
     9601 ctcctccaac gcgggcagag aggagctcca gcgcatcttg ggcaacgccg gagggaaccc
     9661 tgccaggttg gacgacgagg cgctcgccca ggcggccaac cgtacggaac agcaatacct
     9721 gctggctttg aggcaggagg agataccccc ggagttcgag gacgaccatc actacatcat
     9781 cacggcgctg ggcataagat tccaggctac agacaagctc gtgcaggcgg cttcggggga
     9841 tgccagcggg tttgccgggg agctcgcgat ggccgtcgag gactacagga cgagcgatag
     9901 catcgtcgcg aactactaca tcccggccgt caagcgttct ctggagagtg ctggccagac
     9961 gagcgatcag acctacctcg aagaaccgca atcctttatg gattacgggg cgctcggttt
    10021 cgacacggcc ccggtatccg gcaccgcaag gagcgacccg aacgccctgc acggggtgcg
    10081 ggtgacggcc gtcagggtcg cggggaagcc gctgaactct ggcgggaacg tcgtcctgac
    10141 gggggccgat gagccgacct tcgccgttac cgtcgggaac gacggtgagg tggccgagac
    10201 gggcgtcgag gtcgaggtcg tcctcaacac gagagccgag cgccaggccg agagcaagac
    10261 cctagcgcgc ctcgaacccg acaagatggc gacggtcgag gtcggcggct ttatacccgg
    10321 cgaactcgac gagagcgcca gggtcaccgt cgaggccggg cccgtcgaat acgagaaacg
    10381 tacggccaac aacacgctca cggggagggt gacgtttggg atataggagc gctgtgcgaa
    10441 aagaagggat ctgtacaaca gggcttcagg catcaggtta caggcttcag gacgtccttt
    10501 gggatggagc cgaggacccg aaagtggtgg gtactacttc ggtgattggg gcggctggca
    10561 aagggttcga ggagtctgca tggtaacggc aatagtgctg gtcaggacgg agagggagcg
    10621 tgtgcaggaa gccgcccagg ccatgctcgc gatagatggc gttaccgagg cttactccgt
    10681 cacgggcccc tacgatctcg tcgtcatggt ccgtatcccc gacttcgaga gcctggccga
    10741 tgtcgtcccc gaaaggatag cccaggtgcc aggcgtcgcc cgcacggaga ccatggtcgc
    10801 cttccgcgct tactccaact acgacctgga ccgcgcctgg agcttggggt tcgaatagct
    10861 ttcagcccgc tacagccttc ggcttccgct ctcagcccgc ttcggcttcg cctcgctctc
    10921 agccttcagc ttcgttcgtt ctagcctcgc cctgctgcgc acgagcaacc cttgcccccg
    10981 gggtcgggtt ctacgctcat agcgacgttc tgagacgact tggctgaggg ctgagagccg
    11041 ccgaccggag gaaggtggcg ggctgacaag cggaggcgac gccggagcgt gctgaaagct
    11101 cccgaaggga gcgtgctgac tagctctgtt cttctgcgag ggcggggcgg gtgccttcta
    11161 tgaggaaggc gactacgtcg aggaagttac cgctctcttt gtagacctcg cgctggcgct
    11221 ggctgcccgt gccgccttcc actatatcca ggacgccttc tagctcgctc gcgcaaccga
    11281 gatcctgggc gtggggcttc agccgctcga cgaggttacg ggcgaggtct ctggccggga
    11341 ccgtgatgtc gtcgcgggcg tcgtagaaag ccgcgtccat tccgtatctg gcagcgcgaa
    11401 atttgttctc taccgccagg tgcatgttgt agggttcgtg cgggccgact tcctctagta
    11461 cttcggcgac gaggcactgg gtcagggcgg taagggccag ggtatccttc aggttggtct
    11521 gggagtccag gacccggagc tctatcgtcc cgatcttggg gtgcgggcgc acgtcccacc
    11581 aacagaacgt gtagtcctcc atcgtgcctg aggcgaccat aagatccacg taaccctcga
    11641 aggacccgta gtcggggaag gcggggggga ggccggcacg cgggaagctc tcgaagatct
    11701 tcaccctcgc cgactcgaag cccgtatcga aaccctgcca gaaaggagag ttggccgaga
    11761 gcgccaggag gatcggcgtc tgctcggcga gacggttgtg ggcgacgatc gcctcctcgg
    11821 ggcctgggac ggccacgtgg acgtgctggc cgaagatcac ctcgcgctgg gcgatccagc
    11881 gcagcgtcct caagacgttc ttgtagcgct cctggttcgt gaccttctgg tccctgtagc
    11941 gggagaaggg atgggtcccc gcggaggcga gcgaggcgcc gcaggccgcc gcccacgagc
    12001 cgaccctgct gcgtagttcg cgcagatgct tctccacctc ggaggtcgag tcgcagggcg
    12061 gggtctttat ctccattacc gactggaaca gctcgtagga gacggagtcg tgcagctcgg
    12121 caggcagccg gctcatgatc tcctcgatct tctgcaccag ctcaccggtg gtcgcgtcta
    12181 cgatggggag ctcctcctcg accccgaggg agtagtccga agcgccttcc ttgaactcta
    12241 taggcatggc ttcctcctcc gttctccggg ttcaggggaa gtggatctct tgcagccgac
    12301 aattctcaac gtggggcaac atattagcac ttcagctagt cagcacgctt cgggcctttc
    12361 aggctctcgc ttgtcagctt ctcccgcccc gccctcaccc tgccgcgcgc gaggaaccgc
    12421 taccagcggc ggacggattc tacgctcttg gcgaggccac ggaacgactt gctgacaagc
    12481 tgacaaggcg gcgcagccac cgtgctgacg agccgcgaag cggcgtgctg aaatgctgac
    12541 tacctggaat tctttcggac tctcacctgg gcgagcaccg ccccggagat cacgagtcct
    12601 gcgccgaccg a
//
LOCUS       DATMVF010000233         5487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_442331, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000233 DATMVF010000000
VERSION     DATMVF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442331"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VN178_12005"
     CDS             <1..819
                     /locus_tag="VN178_12005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWS81737.1"
                     /translation="SPETKWIWRCHPDLSTPNRHVLDYLLPHIADYDAQIYTMEEYTP
                     PDVHLPGLTLIPPAIDPLSPKNMALSADDARYIVSQFGVDAEKPFLVQVSRFDPWKDP
                     LGVIDAYRLVKEEVPEIQLVLIGSMAHDDPEGWDYWYKTVNYADGDRDIFLFSNLTNV
                     GAIEVNAFQSIADVVIQKSIREGFGLVVTEALWKARPLVAARVGGIPMQVDGGGGILI
                     DTIPEAASACIKLLKDRDFARQMGRHGKEHVRTHYLTPRLLRDDLLLFAKLLGV"
     gene            843..2291
                     /locus_tag="VN178_12010"
     CDS             843..2291
                     /locus_tag="VN178_12010"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWS81738.1"
                     /translation="MARGEGQRIIVVSNRGPVTFARGESGEREHSRGAGGLVTALNAV
                     LRRAENAVWIASAQSEEDVAVSREPAPYEVEDLRIRFVEHDPGAYDLMYNELANPLLW
                     FVQHGLYDLPYSPALGDGTRRAWEEGYVPVNRNFADAVIETTSDEDSSTILLHDYQLY
                     MTPLFIRERLGASAFLSLFVHIPWPEPDLWRVLPRYVREGVLESLLSADVVAFHTERY
                     ARAFVQTAADVLGAETEDGVIFYRGREIWVRAYPISIDPGEFEQLAQGEDVLEEERFV
                     KNLPGKLLLRVDRTDLSKNVVRGFRAYGRMLERHPEMRGEVTFLAQLQPSRTDIREYA
                     AYMEAVGRTAEEVNEAYGTPSWRPVELFMEDNFPRSVAAYKNYDVLLVNAVRDGMNLV
                     AKEAAVINEKNGVLVLSENAGAHEELGEHALTVNPYDIDEQADSIYAALSMPEEERGR
                     RASALRETVESNTIEDWVEAQMQDIEAYRGRR"
     gene            2300..2755
                     /locus_tag="VN178_12015"
     CDS             2300..2755
                     /locus_tag="VN178_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81739.1"
                     /translation="MRIFLMAAAVLIALANAILYSLIGNNTFSNLFDFHLDPWYLCAM
                     YVLCGVFAGLLVAGGLIAFAEKILRETFFARYGLMVLAICIGGAALAILLTTVTFLFD
                     DAAYVPSGAAETSYTLVLVTIAGATLGAIEGVILAFPLAWLLGLIGERD"
     gene            complement(2752..3546)
                     /gene="otsB"
                     /locus_tag="VN178_12020"
     CDS             complement(2752..3546)
                     /gene="otsB"
                     /locus_tag="VN178_12020"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWS81740.1"
                     /translation="MDGREAYERLRRWGEELGSVALLTDIDGTLAPIVPTPDMSEIPD
                     GIRDSLERLSRESLLVAAISGRKTEDALGLLRLEDVVYFGNHGFEILRDGEVEVIPEA
                     LPYLRSVEELERLAREELAPAGAFVEEKGITASVHYRNVGREVGERCVAFVKSEGERL
                     GLRITVGRGVVEARPPIRADKGTAVRTLVEEYRPKKAIFVGDDTTDLDAFRELDRLRE
                     EGSLMEILRVGVASEEGPQEIRSEADIVVDGVDGVGRVLRALLGQG"
     gene            complement(3591..3791)
                     /locus_tag="VN178_12025"
     CDS             complement(3591..3791)
                     /locus_tag="VN178_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HWS81741.1"
                     /translation="MATWTNKQWGAVIGALVLFSMFLIGFEETLVVVLFGIIGYFIGK
                     YLDGEIDTDEIRARAQGRQRGT"
     gene            complement(3809..4369)
                     /locus_tag="VN178_12030"
     CDS             complement(3809..4369)
                     /locus_tag="VN178_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81742.1"
                     /translation="MNALNRLILLVVALLLVAVPVLLLLMNWGLIQADVVDQYTRYRS
                     AVQALGGFQASALTTAARVVIAVVGALVALIALLLLLRELTLGRRVSRSTVMEDTPGR
                     ETIITANAVKTLAESAAREVGAESPSVSLASDGDSYIVFTGIEVPLSENYTELAARAR
                     GNIQRVLGDQGVPLKDVEVTVRGTAL"
     gene            complement(4366..4932)
                     /locus_tag="VN178_12035"
     CDS             complement(4366..4932)
                     /locus_tag="VN178_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81743.1"
                     /translation="MSVFNRIVMTLLFAGLFVLGVYTVVYSFDLFGRSLTSLPISDFS
                     SGLQDFVGRVEDGNLSVLTIITLVLIAILGLIWFIAELRPSTPSNVRVGDGTYVTRGV
                     VGEEVSTAAEQVPDVLGSSVGVKARRSPGARVDLEARVRRGEDLETIRSGLRTTVQEH
                     LSEKGLPVSRLNVKLVEADPRQTKTRVQ"
     gene            complement(4919..5221)
                     /locus_tag="VN178_12040"
     CDS             complement(4919..5221)
                     /locus_tag="VN178_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81744.1"
                     /translation="MSDGIQLARAATEAALATEGVYSMGRGRYAEAATYEGNEKILGV
                     VVGPEDLEIHIVAAYPLPRPIPEIAENIRERVAPQAGGRRTMVVVEDLEVGEDERL"
     gene            complement(5252..>5487)
                     /locus_tag="VN178_12045"
     CDS             complement(5252..>5487)
                     /locus_tag="VN178_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HWS81745.1"
                     /translation="LDLTLTAEYGKSIPQLAEAVRRNVANRVESLVGLRVTEVNITVS
                     NIFFPQQEAEQERQRQIEQERREQEAQEQQRVQ"
BASE COUNT          974 a         1845 c         1642 g         1026 t
ORIGIN      
        1 tcgcccgaga cgaagtggat ctggcgctgc catccggatc tctccacccc caaccggcac
       61 gtgctcgact acctccttcc gcacatagcg gactacgacg cgcagatcta caccatggag
      121 gagtacaccc cccccgacgt gcaccttccc gggctcaccc tgatcccccc ggcgatagat
      181 ccgctctcgc ccaagaacat ggccctgagc gccgacgacg cccgctacat cgtcagccag
      241 ttcggcgtgg acgcggagaa gccttttctg gtgcaggtgt cgcgcttcga cccgtggaag
      301 gaccctctcg gcgtcataga cgcctacagg ctcgtaaaag aggaggtccc cgagatacag
      361 ctcgtcctga tcggctcgat ggcccacgac gatcccgagg gttgggacta ctggtacaag
      421 acggtcaact acgccgacgg tgaccgcgac atcttcctgt tctccaacct cacgaacgtc
      481 ggagccatcg aggtcaacgc cttccaatcc atagcagacg tggtgattca gaagtccatc
      541 cgcgagggct tcgggctcgt cgtgacggag gcgctctgga aggcaaggcc gctggtcgcg
      601 gccagggtcg gcggcatccc gatgcaggta gacggggggg gcggtatcct gatcgacacc
      661 atccccgagg ctgcctccgc gtgcatcaag ctcctcaagg accgcgattt cgcccgccag
      721 atgggccgtc acggaaagga acacgtaagg acgcactacc tcacgccgcg tctcctgcgc
      781 gacgatctcc tgcttttcgc taaactgctc ggggtgtagc agccgcgaac gagaggggga
      841 ccatggcgcg aggcgaaggc caaagaatca tcgtggtctc gaacagggga cccgtaacgt
      901 tcgcccgcgg cgagtcgggc gagcgcgagc actccagggg agcgggtggc ctcgtaaccg
      961 ccctgaacgc ggtcctaagg cgcgccgaga acgcggtctg gatcgcctcc gcccagagcg
     1021 aggaagacgt tgcggtctcc agggaacccg ccccctacga ggtcgaggat ttgcgtatca
     1081 ggttcgtaga gcacgatccc ggggcctacg accttatgta caacgagctc gccaacccgc
     1141 tcctgtggtt cgtccagcac gggctctacg atctgcctta ctcgcccgcg ctaggggatg
     1201 ggacgaggcg ggcctgggag gagggctacg tccccgtcaa ccgcaatttc gccgacgccg
     1261 taatcgagac cacatcagac gaggattcgt ccaccatcct cctccatgac taccagctct
     1321 atatgacccc gctcttcatc cgcgagcgtc tcggagcttc ggcctttctc tccctgttcg
     1381 tccacatccc ctggccggag ccggatctct ggcgggttct gccgcgctac gtgagggagg
     1441 gtgtgctcga gagcctcctc tcggccgacg tcgtggcctt ccacacagag cgctacgccc
     1501 gcgccttcgt ccagaccgcc gccgatgtac tcggcgccga gaccgaggac ggcgtgatct
     1561 tctacagagg acgcgagatt tgggtgcggg cttaccctat ctctatagat ccaggggagt
     1621 tcgagcaact ggcacagggt gaggacgtgc tcgaggaaga gaggttcgtc aagaaccttc
     1681 ccggaaagct cctcctgcgc gtcgaccgta ccgacctctc caagaacgtc gtccgcggct
     1741 ttcgggccta cggcaggatg ctagagcgcc accccgagat gaggggcgag gtcaccttcc
     1801 tcgcccagct ccagccctcg cgcactgaca ttcgcgagta tgccgcctac atggaagccg
     1861 taggcaggac ggcggaagag gtcaacgagg cgtacggcac gccatcgtgg aggcccgtag
     1921 agctctttat ggaggacaac tttccccgct ccgtcgccgc ctacaagaac tacgacgtcc
     1981 tgctcgtcaa cgccgttcgc gacggcatga acctcgtcgc caaggaggcg gccgtcatca
     2041 acgagaagaa cggagtcctc gtcctttcgg agaacgcggg ggcccacgag gagctcggcg
     2101 aacacgcact gaccgtcaac ccctacgaca tagacgaaca ggccgattcc atctacgccg
     2161 cgctctccat gccagaagaa gaacgtgggc gtagggcgag tgcgctgcgc gaaacggtcg
     2221 aatccaacac catagaagac tgggtcgagg cgcagatgca agacatcgag gcgtaccggg
     2281 ggcggcgata agcgcgtcaa tgcgcatctt cttgatggcg gcggccgtgc tgatcgcgct
     2341 ggccaacgcc atactctaca gtttgatcgg caacaacacc ttctccaacc tcttcgactt
     2401 ccacctcgac ccgtggtacc tctgcgcgat gtacgtgctc tgtggtgtct tcgcggggct
     2461 cctggtcgcg ggcggactca tcgcgttcgc ggagaaaata ctcagggaga cgttcttcgc
     2521 gcgctacggt ttgatggtgc tggcgatctg catcggggga gctgcgttgg ccatcctcct
     2581 gaccaccgtc acgttcctct tcgacgacgc ggcctacgtg ccgagcggag ccgccgaaac
     2641 ctcttacacg ctcgttctgg tcaccatagc aggggcgacg ttaggggcga tagaaggggt
     2701 gatcctcgcc ttcccgctgg cctggctcct cggcctgatc ggcgagaggg actagccctg
     2761 gccgagaagc gcccttagca cccttcccac cccgtcgacg ccgtcgacga cgatgtcggc
     2821 ctcgctacgg atctcctgcg gtccctcttc gctcgccacg ccgacgcgca gtatctccat
     2881 taggcttcct tcctcgcgca gccgatccag ctcgcgaaag gcgtcgagat ccgtggtgtc
     2941 gtcgcccacg aaaatggcct tctttgggcg atactcctcg accagggtgc ggacggcggt
     3001 gcctttgtcc gccctgatgg gcggacgcgc ctcgacgacg ccgcggccta ccgtgatcct
     3061 gagccccagg cgttcccctt cgctcttcac gaacgctaca cagcgctcgc cgacctcccg
     3121 ccccacgttg cggtagtgca ccgaggccgt tatgcccttc tcctcgacga aggcgcccgc
     3181 cggggcgagt tcctccctcg caagccgttc caactcctcg acactcctta ggtagggtag
     3241 ggcctcgggg atcacctcga cctcgccgtc gcgcaggatc tcgaagccgt ggttgccgaa
     3301 atagacgaca tcttcgagcc tcaggagccc gagcgcgtcc tccgtcttcc tcccgctgat
     3361 ggccgcgacc aggagactct ccctgctcag tcgctcgagc gagtccctga tcccatcagg
     3421 gatctcggac atgtcgggcg tcggcacgat gggggcgagc gtcccgtcta tgtccgtgag
     3481 caaggctacc gagcctagct cctcgcccca tctcctcaga cgctcgtagg cctctctacc
     3541 gtccaccttg agaccgctag agggatccgt tcgggagcgg ctcattccat ttacgtgcct
     3601 ctctggcggc cctgggcgcg ggcgcggatc tcatcggtgt cgatctctcc gtcgagatac
     3661 ttacctatga aatacccgat tatgccgaat agtacgacga cgagggtctc ctcgaaaccg
     3721 atcaagaaca tcgagaacag caccagtgcc ccgatcacgg cgccccactg tttgttggtc
     3781 caggtggcca tctcctacct tcctttcact aaagtgcggt tcctcttacc gtgacttcga
     3841 cgtcctttag gggcacaccc tggtccccca gcaccctctg gatgttcccc ctggcgcgcg
     3901 ccgcgagttc ggtgtagttc tcggacagag ggacctctat ccccgtgaac acgatgtagg
     3961 agtcgccgtc ggaggcgagc gagaccgaag gggattcggc gccgacctcc ctggctgcgc
     4021 tctcggcgag ggtcttgacg gcgttggccg ttatgatggt ctccctgccc ggcgtgtcct
     4081 ccataacggt gctcctggag accctgcgcc cgagcgtgag ttccctgagc agcagcagca
     4141 gcgctatcag ggccacgagg gcgccgacga cggcgatcac gaccctcgcc gccgtagtga
     4201 gagcggaggc ctggaagccg ccaagggcct ggaccgcact cctgtacctt gtgtactgat
     4261 ctaccacgtc ggcctggatg agcccccagt tcatgagcag tagcagtacg gggacggcga
     4321 cgagcagcag ggccactacg agaaggatga ggcgattcag ggcgttcact gtaccctcgt
     4381 cttcgtctgc ctggggtccg cttcgacgag cttgacgttg agcctgctca ccggtaaccc
     4441 cttctccgag aggtgttcct ggaccgtggt gcgcaggcca gacctgatcg tctcgaggtc
     4501 ttcgccccgc ctcaccctgg cttcgaggtc tacccgggcg cccggacttc gcctggcctt
     4561 cacgccgacc gaggagccga gaacatcagg aacctgctcg gcggccgtgg agacctcctc
     4621 cccgaccacg ccgcgggtca cgtaagtgcc atctcccacg cgcacgttac tcggagtaga
     4681 tggcctgagc tcggcgataa accagatcag gccgagtatc gctatgagaa ccagggtgat
     4741 tatcgtgaga acggagaggt tgccgtcttc cacacgcccg acgaagtcct gcaggccgct
     4801 ggagaagtcg gagataggca gcgaggtcag gctgcgaccg aacaggtcga agctgtagac
     4861 taccgtgtat acgccgagca cgaagaggcc ggcgaacaac agtgtcatta ctatcctgtt
     4921 aaagacgctc atcttcgccc acctcgaggt cctcgactac caccatggtc ctgcgcccac
     4981 cagcctgtgg cgccacgcgc tcccttatgt tctcggcgat ctctggtatc ggccttggca
     5041 acgggtaggc ggcgacgatg tggatctcca gatcctctgg accgaccacc actcccagga
     5101 ttttctcgtt tccctcgtag gtcgcggcct cggcgtagcg cccacgcccc atggagtaaa
     5161 caccttcggt cgcgagggct gcttcggtcg cggccctcgc gagttggatt ccgtctgaca
     5221 agataagccc tgctctcgga tgaaccgctc tttactggac ccgctgctgt tcctgggcct
     5281 cctgctcgcg gcgctcctgc tcgatctgcc tttggcgctc ctgctccgcc tcctgctggg
     5341 ggaagaagat gttggagacc gttatgttga cctcggtcac gcgaaggccg accaggctct
     5401 ccaccctgtt ggccacgttg cgccgcacgg cctcggcgag ctggggtatc gattttccgt
     5461 actcggccgt cagcgtcagg tcgaggg
//
LOCUS       DATMVF010000234         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_577545, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000234 DATMVF010000000
VERSION     DATMVF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577545"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VN178_12050"
     CDS             <1..752
                     /locus_tag="VN178_12050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWS81746.1"
                     /translation="GPGHAGAIVYGLPEEVPVVAVGGDGTVHEVAAACAGTGRTMGVL
                     PAGSGNDYVKALGVGTNLRRALGILVEGKVRVVDTAEVNGVPFNNGLGIGFDAEVAAG
                     VALAPTYLGGMGRYLWSVGRLLKDFRCHEATLVIDGEVVEAKTILVAVALGTTYGSMF
                     RLAPGAILDDGLFDVIWSEEVNRIEVLRLIPAALGGTLSKHRKVHSARARKIAIELSE
                     EIPAHVDGEMLAHTRHFEARVLPGSLRILAP"
     gene            996..1835
                     /locus_tag="VN178_12055"
     CDS             996..1835
                     /locus_tag="VN178_12055"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWS81747.1"
                     /translation="MFPGQGGGVSEPAPEMVPEIRRVVGERIPDQVKIFVRAARDADE
                     SRTMDVSPDVVAGHSLGEYGAAYAAGCFDFETGLWLVAQRDHFLAEAARECPGGMVAI
                     LRTDPEEVEKALGGPGGPVVANYNSPRQTVVSGLRDALGDAVSKIRGRKIPLNVSFAA
                     HSPYVAVAGEKMRGVLDSVEFQVPQVPIVSAMDGAVLTKADAVKEALKEQMVAPVRWV
                     RVVETLAKMRVEEWIELGGGGVLTRMLKDFELPSLNGITFEDLRARLYGQAQNLLPRQ
                     EGE"
     gene            1987..>2465
                     /locus_tag="VN178_12060"
     CDS             1987..>2465
                     /locus_tag="VN178_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWS81748.1"
                     /translation="MAELTIPYWEVVLRLVLASLLCGAIGFERETRDQPAGFRTHILL
                     GLGAALFTLVSAYGFEPFTRAALGSGGLQFDPTRIAAQIIAGVGFLGAGAIIRQGRDV
                     RGLTTAASLWAVSAIGMAVGAGYLFGAAAATVLTMATLYALRSFRSSIISPLRLDSAG
                     "
BASE COUNT          416 a          762 c          837 g          450 t
ORIGIN      
        1 gggggcctgg ccacgcgggc gccatcgtgt acgggctgcc cgaagaggtg ccagtggtgg
       61 cggtcggtgg agacggtacg gttcacgagg tagcggcggc gtgcgccgga acgggccgga
      121 ccatgggtgt gctgcccgcc ggcagcggca acgactacgt caaggcgctc ggggtgggca
      181 cgaatctccg gcgggcgctg gggatactcg tcgagggaaa agtccgcgtt gtcgatacgg
      241 cggaggttaa cggggttccg ttcaacaacg gcctcggtat cggcttcgat gccgaggtcg
      301 cggccggtgt cgccctggcg cctacgtatc tcggagggat gggaaggtac ctgtggtcgg
      361 tcgggcggtt actcaaggat tttcggtgcc acgaggccac cctcgtcatc gacggcgagg
      421 tcgtcgaggc caagaccata ctcgtcgccg tggccctggg caccacgtac ggctcgatgt
      481 tccggctcgc gcctggggcg atactggacg atggtctgtt cgatgtgatc tggtccgagg
      541 aggtgaaccg gatcgaggtt ctgcggctca taccggcggc gttagggggc accctatcga
      601 agcatcggaa ggtgcattct gcccgtgcgc ggaagatcgc gatagagctc tccgaagaga
      661 taccggcgca cgtagacggt gagatgctcg cgcacacccg ccacttcgag gcgcgggtgc
      721 tgccaggatc cctgcgaatc ctggcaccat agcacttcgg ctggtcagca cgctccgccc
      781 ttcgggcttc gcttgtcagc ccggtggctt tgccgccttg tcagcaggtc agcaaggacc
      841 tgcccatgcg aaagctgaca tgctgaccag cgcgggttgg gaaaggtgaa acctccccaa
      901 cccgcctcct tgtggtacaa cttttcccta ctctttaggg tagggtcgta tctccgagtg
      961 ttatcgagga ctggcgcata gagatgagag cgttcgtgtt tcccgggcag ggtggcggag
     1021 tatccgaacc cgctcccgag atggtgccgg agatccggcg cgtcgtcggg gagcgtatcc
     1081 ccgaccaggt aaagatcttc gtccgcgcgg cgcgggacgc tgacgagagc cgcaccatgg
     1141 atgtgagtcc cgacgtggtg gccggccact ctctcggcga gtacggcgcg gcgtacgcgg
     1201 ccggatgctt cgacttcgag acgggcctct ggctcgtagc gcagcgcgac cacttcctcg
     1261 ccgaggccgc cagagagtgt cccggcggga tggtcgcgat actcaggacg gatcccgaag
     1321 aggtcgagaa ggcgctcggc ggacccggcg gccccgtggt agccaactac aactcaccgc
     1381 gccagacggt cgtctccggg ctgcgcgacg ccctcgggga cgccgtctcc aagatcaggg
     1441 ggcgcaagat cccgctgaat gtctccttcg ccgcccactc tccgtacgtg gcggtggccg
     1501 gcgagaaaat gcgcggagtc ctggattccg tcgagttcca ggtgccgcag gtcccgatag
     1561 tgagcgccat ggatggtgcg gtgctaacca aggccgacgc cgtcaaggag gcgctcaagg
     1621 agcagatggt cgcccccgtg cgctgggtca gggtcgtcga gacgctggcg aagatgaggg
     1681 tggaggagtg gatcgagctg ggcggcggcg gcgtcctgac gcggatgctc aaagacttcg
     1741 agctgccttc tctaaacggc atcaccttcg aggaccttcg cgcccgcctc tacggacagg
     1801 cccagaacct cctgccgcgc caagaaggag aataggctac aggcatcaga atcttgggac
     1861 tcatctccat aacggaacac gtatcggcaa tctttctccc ggtgggatcg taaggtcagg
     1921 tgccatgcac acagtatttc ctgaagcctg taacctgatg cctgaagcct tcaaagcaag
     1981 atccgcatgg ccgaactgac gattccctac tgggaggtcg ttcttcgtct cgtcctggca
     2041 tccttactgt gcggcgcgat cggtttcgag cgggagaccc gcgaccagcc ggccgggttc
     2101 aggacgcaca tcctgctcgg cctcggcgcg gcgctcttta ccctggtctc ggcctacggc
     2161 ttcgaaccgt tcacgcgggc ggcgctcgga agtggaggtc tccagttcga cccgaccagg
     2221 atcgccgccc agatcatagc cggcgtcggc tttctcgggg cgggcgccat aatcaggcag
     2281 ggcagggacg tgaggggcct taccaccgcc gcgagcctct gggcagttag cgctatcggt
     2341 atggccgtcg gcgcaggcta tctcttcggt gcggcggctg caacagtcct gactatggcc
     2401 acgctctacg ccctgcgcag tttcagatcc tccatcatct ctcccctgcg cctcgactcc
     2461 gcagg
//
LOCUS       DATMVF010000235        10799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_492976, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000235 DATMVF010000000
VERSION     DATMVF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492976"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..668
                     /locus_tag="VN178_12065"
     CDS             270..668
                     /locus_tag="VN178_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81749.1"
                     /translation="MPKSPHKRSKDPAAKAASARRRGGRLRVLMANEPRAYREGIAAV
                     IGQLRPEVEIEIVEPEALDPAIERFSPDMVICSKATEALKSRVRVWVELYPENASLSV
                     ASIGGRRVEYAEIQLPDLLSIVDKAEELAD"
     gene            761..1060
                     /locus_tag="VN178_12070"
     CDS             761..1060
                     /locus_tag="VN178_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81750.1"
                     /translation="MANELTSYRQSIAIVIRELHPNVEVLEVHPETLDREVARVGPDL
                     VICSSVTTVVEELAAHWIELYPECSPFSTFCIAGERRAMEDVQLSDLLRACARVL"
     gene            complement(1047..1754)
                     /locus_tag="VN178_12075"
     CDS             complement(1047..1754)
                     /locus_tag="VN178_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81751.1"
                     /translation="MAEREFRLPGSPYEELVNIIVAYGTRDEAARAGDVGKLDSVHQS
                     SVSRNNAFLTEIGVLQGESKKLITRRGRSLAVALAHNDNAGVRSNWRAIVAASEFLQN
                     VVSAVKLREGMLYPTVQAYIAHAAGQPRNKPVMNGAGAIIEILKASGMLKEEAGELVA
                     TFDERPEDIVSEDDSPAKTSESKESVVTATVGGAPADVPTSPTVSIHVQVRCTADEIE
                     DLAPRLKALLRELSTEP"
     gene            complement(2131..3339)
                     /locus_tag="VN178_12080"
     CDS             complement(2131..3339)
                     /locus_tag="VN178_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HWS81752.1"
                     /translation="MEYEKENRKGKGVAFVLAVYAASRLFYLISGSLLAHLVPTSGFQ
                     RVTLDVPPGSMNIWSHWDGEHYVRLAMGGYLTPPDNVSPAFFPLYPLLMRSFAELFGG
                     PISREVLSVWGPLISLLFLPFAFYFIYNIAVEGWGERVARDTVLILALFPTTFFLNAA
                     YTESLFLALSAGSLWAMRVRKDLLLACVLAALAAATRNVGIFLVVPLLYEWIKDIERF
                     RWRGIYLLLAPGGLLAYMGYLWVRFGDPLLFYSAQESWGRQATGPLDTASRAWGTAVE
                     GAGRLLEYDLWAHPSFGNAANRLAGAGDFFNLAFFVFATVVLLAGLRYLPLSLTVYGL
                     LLVAPAALFGTPGSPLMGAPRYVLVAFPVFIVLALLFKNRVLLGGWLVVSTLISLAMC
                     GLFVSWRFVA"
     gene            complement(3392..5458)
                     /locus_tag="VN178_12085"
     CDS             complement(3392..5458)
                     /locus_tag="VN178_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81753.1"
                     /translation="MISDLLYALPAAFLIGVLPGWFWTRCLLASGDRAEQLAYTVALS
                     LTLVPTVALAQTYLFATGVTFGVTVVSVALVSLTGLGAYLLFGAAKESEEPLSPPPSP
                     PGFATLAPLAAALALALLMFLGLASTEWLMIPIAVLVIAGGIAYFSGGREREAPPAGD
                     GGSVSAVWRYPLLSLVLLLVLARGYLGPIVNGWSYPRGVDRYEHAVMAGMMMREGSNE
                     AFMLYPPGFHLLTAMISRLSGIEPLEVFPSLAPMLPLLGALALYALAKRLWGWEYGVA
                     AALTAGLLLGGTYLHFEEARYPNFVGEYFLIVLAIAALVGMYAAPSARAGLLLALLGS
                     STVLYHQIAGYSLAVLLGLVCVLFLPYLLLRERRKGVYLALSLALLGLLSVAYAWDTY
                     DLPSLVAGMLGASETGRGGEAVAMAIGTKPANGPGHFLMTMSHPVLWLGLLGLALMLA
                     GGKDAKGTPDILARLTLLVWTLLLFFGSLTSYSGFPDRFERDLGVPLALLAALTLVTL
                     VRLSTRSGGPAVYVAALFAVTLSITLVGAQAVLSLEQAAGPSARPKDRPAPAEVVAAG
                     TWLERHNEGGSIVATPYLEYVPSRAMLAMGGYTRMQSYDAARIRRARDLPPFGAPPLW
                     DALWVLEHPASGRTARLLEKNDVRYVVFHKGYPWIDWRPFALQSDLYTTVYENGSVII
                     FAPRQN"
     gene            5731..7380
                     /locus_tag="VN178_12090"
     CDS             5731..7380
                     /locus_tag="VN178_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWS81754.1"
                     /translation="MKVVDQTKRILFVALYALLGLAVVCALSACFPSAEGQQAEDQKQ
                     EPKRPNVILILTDDLALNDLNARALRHMPNLESLLIEEGTTFDNAFVTNSLCCPSRAT
                     ILRGQYTHNHEILSNDPPRGGFQKFRYVGHEDSTMATWVKEQGYRTAFFGKYLNGYKE
                     TYIPPGWDEWYAVTGNYLSHTFNENGHLVSYEPESYYDTDVMSDKASDYITRTAGADP
                     PFFTADRPFLMWIGTKAPHQPASPAPRDENAYPAVSLPRPPSFDEKDVSDKPDWVSDN
                     PPLSLEQKRYMEELYRKRLQSMLAVDDMIGDLIGALRASGELDNTYIVFTSDNGFHLG
                     QHRLGAGKWTPYEEDIRVPLIVRGPGVPEGETLHHMVLNNDLAPTFADLAGAEPPSFV
                     DGRSLKPLLKDDPTPLRDWRQRFVIESVAERSGVPHPPFITESTVVPLLTGDPLPGDW
                     RRTSAASAQSSEEWGRPWLKALRTNNYLYVDYKTGEHELYDLRKDPHQLDNIYATAPP
                     EITQRLETQLDALRQCSAEDCRTAESEKQGKRPTGRRPSGG"
     gene            complement(7381..8928)
                     /locus_tag="VN178_12095"
     CDS             complement(7381..8928)
                     /locus_tag="VN178_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HWS81755.1"
                     /translation="MLAGLGVFALVLVLYLETLAPTVLYLQDPELLDAVMLQMQVSVL
                     GIAHPTGYPTYLMLTHLFTYLPFGDPAYRVNLGSAFYAALAVLAVYAAGLLLGRRIVA
                     AATGALVFGLGTALWSQAVIAEVYTLNALLVSVTIVVLLLWREHRQDRYLLLSAFLVG
                     LCLTNHLTSGLLLPAGLLFVALVDWRRLVDFRLVLGGAGLFVLGLIPYLYLPIRAAMD
                     PPMEANNPTDFGRFWYVISGGNLTGSFFSFGPLEMPARMVFYWEHLLDNMPFLVIMVA
                     LPGAVFLLLRDRAVGIFLGVLFFGWLFHAALNDIPDIDLYFIPTYLVLSLWAAAGIGT
                     LLAEVETLVSGSPGVSKGAILGVLSAMVLVLPLLGVSETYAKNDMSGVYRGRQEIEAV
                     ADNAAPNATILHHRSGMWYMVLVEKRRRDLTIVDPFYHNKEVEYADIVWPDDIDLATT
                     DRRYGTDDLSGVSTAKKAAKEGRVYVLDQGSTDPERFRNAGFEVVPVRSGLLYELVPP
                     GREPYAG"
     gene            9329..10537
                     /locus_tag="VN178_12100"
     CDS             9329..10537
                     /locus_tag="VN178_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWS81756.1"
                     /translation="MKVLVLGGDGFCGWPTSLHLSAQGYEVAIVDNLSRRKIDIELEC
                     ESLTPIRPTGERLAVWEELTGNRIDFHNINVAKNYRQLYDLLLEWQPDAVIHFAEQRA
                     APYSMKSSYHKRYTVDNNLNATNNLLAAITESGLDTHVVHLGTMGVYGYGSAGMQIPE
                     GYLTVKVETDGGELVEQEILYPPNPGSIYHMTKTQDQLLFAYYNKNDLTRVTDLHQGI
                     VWGTQTEETRLDERLINRFDYDGDYGTVLNRFLMQAAVGYPLTVHGTGGQTRAFIHIQ
                     DTVRCIQLALENPPERGDRVNVYNQMTETHRLRDLARLVSERTGAEIDYLQNPRNEAA
                     ENDLHVRHDRFLDLGLQPTTLSEGLLEEVTEIAGRYAHRCDTSKIPATSLWRPQVERV
                     HKAPAEPSTL"
     gene            10534..>10799
                     /locus_tag="VN178_12105"
     CDS             10534..>10799
                     /locus_tag="VN178_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81757.1"
                     /translation="MSLAAGQVSIAGLVIAALFAGYGTYRYRAGGSRLDLLLSLLIAL
                     GVVLVSVVPQIFGIFTRLLGLEDRGFALLSIAVLLLFGLFLNLLG"
BASE COUNT         2181 a         3458 c         3441 g         1719 t
ORIGIN      
        1 aagctgcaca tcaccgtgga gaccgaacgc acccacatgg tcaacatact gggcaagctc
       61 gatgtccact ccaggctaca ggccctggtg ttcgccgccc gccacggcct tgtcgagatt
      121 cgttaggcgt tcgtggccct cgatgcagga ccagcgacgt tgccggccga ctttatgtgg
      181 ggctcaccgt gctagaatca atgatgctcg tgtcgatgac cgcgcagaca ggttcgaagc
      241 acgaagctac ccgaggagac acgcccttct tgccgaagtc gccccacaag cgctccaaag
      301 accctgcggc gaaggcggcc tctgcgcggc gccggggggg taggctgcgg gtcctgatgg
      361 ccaacgagcc gcgcgcctac cgcgagggta tagcggcggt catcggccag ctacgacccg
      421 aggtcgagat agagatcgta gaaccggagg ccctggatcc cgccatcgag cgcttctcac
      481 ccgacatggt catttgcagc aaggccacgg aagcgttgaa gagccgtgtg cgggtgtggg
      541 tggaactcta ccccgagaac gcctcgctgt cggtagcatc tatcggcggc agacgcgtgg
      601 agtacgccga gatccagctc cccgatctgc tctccatcgt ggacaaggcc gaggagctgg
      661 cggactagcc cattagggct agaaaaagat ccccctattt catgatgctg gacgtccttc
      721 cagaggtcac cattgcagta tgggcttgag atgcatcctc gtggccaacg aacttacttc
      781 gtatcgtcag tcgatagcca tcgttatcag ggagctacat ccgaacgtgg aggtgctcga
      841 ggtccacccc gagaccctgg accgggaggt cgcgcgcgtc ggccccgacc tggtgatctg
      901 cagcagcgta accaccgtcg tcgaggagct ggccgcgcac tggatcgaac tctaccccga
      961 gtgcagcccc ttctcgacgt tctgcatcgc cggagaacgc agggcgatgg aagacgtcca
     1021 actctccgac ctcctccggg cgtgcgctag ggttctgtag agagctccct gagcagcgcc
     1081 ttcagccgcg gggcgagatc ctcgatctca tcggccgtac acctgacctg gacgtggatg
     1141 ctgaccgtag gggatgtagg cacgtccgcc ggggccccgc ccacggtcgc ggtgaccacg
     1201 gattccttcg actcggaagt ctttgcgggc gaatcgtcct cagagacgat gtcttcgggc
     1261 cgctcgtcga aggttgcgac cagctccccg gcctcctctt tgagcatacc ggaggccttc
     1321 agaatctcga tgatggcgcc ggcgccgttc atcacgggtt tgttgcgggg ctggcccgcg
     1381 gcgtgggcta tgtaagcctg gacggtgggg tagagcattc cctcgcgcag cttcacggcg
     1441 gagacgacgt tctgcaagaa ctcgctggcc gcgactatcg cgcgccagtt gctcctcacc
     1501 ccggcgttat cattgtgcgc cagggccacg gccagagagc gtccgcggcg tgtgatcagc
     1561 ttcttgctct ctccctgcag aaccccgatc tccgtgagga aggcgttgtt gcggctgacg
     1621 ctcgactgat gcaccgagtc tagcttgccc acgtccccgg ccctcgccgc ctcgtcgcgg
     1681 gttccgtacg ccacgataat gttgaccagc tcctcgtagg gagagccagg aagcctgaac
     1741 tccctctccg ccatcccgac acctcctccc ccaagacgtt tcccatagaa cacgttcggg
     1801 ccattataaa gcacggcgct tcgcgcctct cagcacgctc ccttcggtcg ctcgtcagca
     1861 cttcagcacg cttcgggtct tcgaccctcg cttgtcagca ggtcagcctc tcctgctccg
     1921 gacctcgcct tgccgcgcac gacgaaccgc ttcaagcggc agtcacgttc tgcgctctcg
     1981 gcgaggttac gaaacgactt gctgaagtgc tgacaagccg ccgaccggag ggaggcggcg
     2041 ggccgacaag cggaggcgaa gccggagcgt gctgaaagcg aagcccgcag ggcgtagcgt
     2101 gctgagagcg ggttgatccc caacccgctc tcatgcgacg aagcgccagc tcacgaaaag
     2161 tccgcacatt gcgagggaaa tgagggtgct cacgacgagc catccgccga ggagcaccct
     2221 gttcttgaac aggagcgcga gcacgatgaa aacggggaat gcgacgagca cgtaccgtgg
     2281 ggcgcccatc agcgggctcc ccggcgtccc gaagagcgcg gcgggggcga cgagcagcaa
     2341 accgtagacg gtcagggata ggggcaggta tctgagcccg gcgagcaata cgaccgtggc
     2401 gaagacgaag aaggccaggt tgaagaagtc gccagccccg gcgagtcgat tcgccgcatt
     2461 gccgaacgag ggatgggccc aaaggtcata ctcgaggagt ctgcccgccc cttcgacggc
     2521 cgttccccag gctctgctcg cggtgtcgag cggccctgtg gcctgccttc cccagctctc
     2581 ctgggcagag tagaagagga gagggtctcc gaagcgtacc cacagataac ccatatatgc
     2641 caatagccca ccaggcgcca gcagcaggta gatcccccgc caacgaaatc tctctatgtc
     2701 ctttatccac tcgtacaaga ggggaacgac cagaaatatc cctacattgc gcgtggcggc
     2761 ggcaagagca gccagtacac acgcaagcag caggtccttc cttaccctca tcgcccagag
     2821 ggagcccgct gagagcgcta gaaagaggct ctccgtgtac gcggcgttca agaagaacgt
     2881 ggtagggaag agggccagga taaggacggt atctctcgct actcgctccc cccagccctc
     2941 gacggcaatg ttgtagatga agtagaaggc gaaaggcaga aagagcaggg agatgagagg
     3001 tccccacacg gagaggactt ccctggagat gggaccgccg aagagctcgg cgaaggatct
     3061 catgagcagc gggtacaggg ggaagaaggc cggggagacg ttgtcagggg gggtgaggta
     3121 gccgcccatg gccagtctga cgtagtgctc accatcccaa tgcgaccaga tgttcatgct
     3181 ccccgggggc acatccaggg ttactctctg gaagccagag gtcggcacga ggtgggcgag
     3241 cagagagccc gagatcaggt agaacagccg gctcgcggcg tagaccgcga gcacgaaggc
     3301 cacgcccttg ccctttctgt tctctttctc gtactccacc accggacctc cccagaagct
     3361 gcgcagggga ttatagtggc cccggcggtt gctagttctg gcggggcgcg aagatgatga
     3421 cgctgccgtt ctcgtagacc gtcgtgtaga ggtcgctctg gagcgcgaaa gggcgccagt
     3481 ctatccacgg atagcccttg tggaacacca cgtagcgcac gtcgtttttc tccaggagtc
     3541 gtgcggtacg cccgctcgcc ggatgctcca gcacccacag tgcgtcccac agcggagggg
     3601 ccccaaaggg cgggagatcc ctcgcccgcc ttatcctggc cgcgtcgtag gactgcatcc
     3661 gcgtgtatcc gcccatcgcg agcatggccc ggctcgggac gtactccagg taaggagtcg
     3721 cgactatgct ccctccttcg ttgtgccgct cgagccacgt ccctgcggcg acgacctcgg
     3781 ccggagcagg cctgtctttg gggcgcgcgc tgggtcctgc ggcttgctcc aggctcagta
     3841 cggcttgggc gcctacgagg gtgatcgaga gggtgacggc gaatagggcc gccacataga
     3901 cggcgggtcc gcccgagcgg gtcgacagcc gcaccagggt gacgagggtg agggctgcga
     3961 gcagcgccag gggaacgccg aggtcacgct cgaagcggtc ggggaaaccg ctgtaggagg
     4021 tcaggctgcc gaagaacagc agcaaggtcc acaccagcag ggtgaggcgc gcgaggatgt
     4081 caggggtgcc cttcgcgtcc ttgccgcccg cgagcatgag cgccagaccg agcaaaccga
     4141 gccagagaac ggggtgtgac atcgtcatga ggaagtgtcc gggcccgttc gccggcttgg
     4201 tcccgatcgc catcgccacg gcctcccctc ccctccccgt ctccgatgcg ccgagcatcc
     4261 ctgccaccag gctgggcagg tcgtaggtgt cccaggcgta ggcaacggag aggagaccga
     4321 ggagcgcgag cgagagagcg aggtacaccc ctttcctgcg ctccctaagg agcaggtagg
     4381 gcaggaagag aacacatacc aaaccgagca gcacggcgag gctgtagccc gctatctgat
     4441 ggtagaggac cgtcgaggag ccgagtagcg ctagcaacag accagcgcgc gccgacggag
     4501 cggcgtacat cccgaccagg gcggctatgg caagcacgat caggaaatac tcccctacga
     4561 agttcgggta gcgggcctcc tcgaagtgca ggtacgtgcc ccccaggaga aggcctgcgg
     4621 tgagggccgc cgccaccccg tactcccagc cccagaggcg cttagccagc gcgtaaaggg
     4681 ccagcgcacc caagagaggg agcatgggag caaggctcgg gaagacctcc agcggctcga
     4741 tgccgctgag gcgcgagatc atggcggtga ggaggtggaa gcccggcggg tagagcatga
     4801 acgcctcgtt cgacccttcc ctcatcatca tgcccgccat caccgcgtgc tcgtagcggt
     4861 ccacgcccct tgggtaggac cagccgttta cgatagggcc caggtagcct cgcgcgagca
     4921 cgagcaggag caccagggag agcaacgggt agcgccacac cgcggagacc gagcccccat
     4981 ctccggccgg gggcgcttct ctctcccgcc cgcccgagaa gtaggcgatc ccgccagcga
     5041 tcacgaggac agctatcggg atcatcaacc attctgtgga cgcaaggccg agaaacatca
     5101 ggagggccag ggcgagggcc gcggcgagtg gcgcgagcgt cgcaaagccg ggaggcgaag
     5161 gcgggggcga gagtggttcc tcgctctctt tggctgctcc gaagagcaaa tatgccccaa
     5221 ggcccgtcag ggataccagc gccaccgaga cgacggtcac cccgaaggtg acgcccgtcg
     5281 caaagaggta ggtctgcgcg agcgcgaccg tgggcacgag cgtgagggag agcgccacgg
     5341 tgtaggcgag ctgctcggcg cggtcgcccg aggccagcag gcaccgcgtc cagaaccatc
     5401 ctggcagaac cccgatcagg aacgccgcgg ggagggcata gagcagatca gagatcaaac
     5461 gccgctccgc gggagccggt ttgcggcgcc cctttcgggt ggagcgtagg gcccaccggc
     5521 cgcaaagagc gaacggttgc ctccggctcc ttgcgcttcg ccggtcttct ggaattggac
     5581 tcgcccatcg cgggaatgta taacacttaa cctggatgaa acacagaccc ggaaggtcga
     5641 gcgcttcgaa gaagcttgca aagaccctcg cccgggtaaa gctaaaggat atccgaacgg
     5701 cgcaccggtg cgccgtccgg cgatgcggac gtgaaggtag tggatcagac caagagaata
     5761 ctctttgtag ccttgtacgc gctcctcggg ctggccgtgg tctgcgccct ctcggcctgc
     5821 ttcccttcgg ctgaggggca acaggccgag gaccagaagc aggagccgaa acgacccaac
     5881 gtcattctca tcctcaccga cgatctggcc ttgaacgacc tgaatgcccg cgctctcagg
     5941 cacatgccca acctcgagtc gctgcttata gaggagggca cgaccttcga caacgcgttc
     6001 gtgaccaact ccttgtgctg tccttccagg gccaccatcc tgcgcggcca gtacacccac
     6061 aaccacgaga ttctcagcaa cgacccgccg cgcggcggct tccagaagtt ccgctacgtc
     6121 ggccacgaag actcgacgat ggccacttgg gtcaaggagc agggctacag gacggcgttc
     6181 ttcgggaagt atctcaacgg ctacaaagag acctacatcc ctccggggtg ggacgagtgg
     6241 tacgcggtga ccggcaacta cctcagccac acgttcaacg agaacggcca tctggtcagc
     6301 tacgaacccg agtcctacta cgataccgac gtcatgtcgg acaaggcctc cgactacatc
     6361 acgcgcacgg cgggggccga cccgccgttc tttacggcag acaggccgtt cctgatgtgg
     6421 atcggtacga aggcccccca ccagccggca tccccggccc ccagggacga gaacgcctac
     6481 cccgccgtct cgctgcctcg tccgccttcg ttcgacgaga aggacgtctc cgacaagccg
     6541 gactgggtga gcgacaaccc accactgagc ctggagcaga agagatacat ggaagagctc
     6601 taccgcaagc gtctacagtc catgctggcc gtggacgaca tgataggaga cctcatagga
     6661 gcgctgcgcg ccagcggcga actggacaat acctacatag tgttcacctc ggacaacggc
     6721 ttccacctgg gccagcacag gctgggggcc ggaaaatgga cgccctacga ggaagacatc
     6781 cgcgtaccgc tcatcgtacg cggtcccggc gtgcccgaag gcgagaccct gcatcacatg
     6841 gtgctaaaca acgacctcgc gccgaccttc gccgaccttg ccggggccga gccgccgtcc
     6901 tttgtggacg ggcgctcgct gaagccgctg ctaaaggacg accccacgcc cctgcgagac
     6961 tggcgccagc gcttcgttat cgagtccgtc gcggagcgca gtggcgtgcc acacccgccg
     7021 tttatcaccg agagcacggt ggtgccgctg cttacgggcg acccgctgcc gggcgactgg
     7081 cggcggacgt cggccgcgag cgcccaatcg agcgaggaat ggggcaggcc gtggctcaaa
     7141 gccctccgta ccaacaacta cctgtacgtc gattacaaga ccggcgaaca cgaactgtac
     7201 gacttgagga aagatcccca ccaactggac aacatctacg cgaccgctcc gcccgagatc
     7261 acgcagcgac tcgaaacgca actcgatgcg ctcaggcagt gctccgctga ggattgtcgc
     7321 accgccgaga gcgagaagca gggaaagcgg ccgaccggaa ggcgaccaag cggagggtag
     7381 ctaaccggcg tagggctccc tgcccggcgg caccagctcg tagaggaggc ccgaccttac
     7441 ggggaccacc tcgaagccgg cgttcctgaa gcgctcgggg tccgtggacc cctggtcgag
     7501 gacgtacaca cgaccctctt tcgcggcctt cttggccgta ctcacccccg agaggtcgtc
     7561 ggtcccgtag cggcggtccg tggtcgcgag gtctatatcg tcgggccaga ctatgtccgc
     7621 gtattcgacc tctttgttgt ggtagaaggg gtcgacgatg gtcaggtccc tgcgacgctt
     7681 ctcgacgagg accatgtacc acatgccgct cctgtggtgc aggatggtgg cgttcggggc
     7741 cgcgttatcg gcgacggcct cgatctcttg gcgcccccgg tagacgccgc tcatgtcgtt
     7801 cttcgcgtag gtctcgctca caccgagcaa cggcaacacg agcaccatcg ccgagagcac
     7861 gccgaggata gcccccttcg ataccccggg tgagccggag acgagcgttt cgacctcggc
     7921 gagcagcgtc ccgatcccgg ccgcagccca aagcgagagc acgaggtagg tcggtatgaa
     7981 gtagaggtcg atgtcgggga tgtcgttcag ggcggcgtga aagagccagc cgaagaagag
     8041 aaccccgagg aagataccca ccgcgcggtc cctgagaagc agaaagacgg cgcccggcaa
     8101 cgcgaccata atcacgagaa agggcatgtt atcgagcagg tgctcccagt agaagaccat
     8161 cctcgccggc atctccagag gcccgaagga gaagaacgag ccggtcaggt tccccccgct
     8221 gattacgtac cagaagcgcc cgaagtcggt agggttgttc gcttccatgg gcgggtccat
     8281 cgcggccctg atgggcagat acaggtaggg aatgagtccc agcacgaaga gtccggcgcc
     8341 ccctaagacg agcctgaagt cgaccagcct gcgccagtct acgagggcga cgaagaggag
     8401 gccggccggc aagagcaatc cgctggtgag atggttggtc aggcagagcc ccacgaggaa
     8461 ggccgagagc agcaggtagc ggtcttggcg gtgctcgcgc cagagtagca ggacgacgat
     8521 ggtgacggat acgagcaggg cgttgagggt gtacacctcc gcgataacgg cctgggacca
     8581 gagggccgtc cccagcccga aaaccagggc gccagtcgca gcggccacga tccgccggcc
     8641 gagcaagagc ccggccgcgt agacggccaa cacggccagc gctgcgtaga aggcggaacc
     8701 gaggttcacg cggtaggcgg gatccccgaa cggcaggtag gtgaagaggt gtgtcagcat
     8761 caggtaggtc gggtagccgg tagggtgcgc gatgccaagc accgagacct gcatctgcag
     8821 catcacagcg tcgagcagct cggggtcctg tagatacagg accgtgggag ccagggtctc
     8881 taggtagagg accaggacga gcgcgaagac acccagcccg gcgagcaaca gaccagcttt
     8941 gcgtagaggt tccccacctc cggcagacgc gtccgcagac cgagaagcag tcctttcctg
     9001 ttccagtgcc acgtcctgag acatcctttg ccgatcttca gggcgggggc tattgtaccc
     9061 agccgggcca gcgatgttca tttctattaa tctgcgttaa tatagcgtcc gccgggtatg
     9121 ctaaccggta ggttgtcaga gccatgtccg gaggtgaacc ggcggggccg gcttgccgga
     9181 ccgcaggaag agcggaacag agcgtttaca ctgattcagt attcgttcaa cgatttttcc
     9241 gttacaatcg tcggctggca tgttcggttc gggcctgact ctgaacgaca aaatggatgt
     9301 atcgtagagc agtaagggag tgcgattaat gaaggtattg gttctaggcg gcgacggctt
     9361 ctgcggctgg cccacctcgc tgcacctctc ggcgcaagga tacgaggtgg ccatcgtcga
     9421 caacctctcc cggcgcaaga tagacatcga gcttgaatgc gaatccctca ccccgatccg
     9481 ccctacgggc gagcgcctcg cggtctggga ggagctaacc ggcaaccgga tagacttcca
     9541 caacatcaac gtcgccaaga actaccgcca gctctatgat ctgctcctcg agtggcagcc
     9601 cgacgcggtc atccacttcg ccgagcaacg ggctgccccc tattcgatga agtccagcta
     9661 ccacaagcgc tacaccgtgg acaacaacct caacgcgacc aacaacctgc tggcggcgat
     9721 caccgagagc gggctcgaca cccacgtggt gcatctgggg accatgggcg tctacggcta
     9781 cggctcggca gggatgcaga tccccgaggg gtatctgacg gtcaaggtcg agaccgacgg
     9841 gggcgagctg gtggagcagg agatactcta cccgccgaac ccgggcagca tctaccacat
     9901 gaccaagacc caggatcagc tcctgttcgc ctactacaac aagaacgacc tgacgcgcgt
     9961 caccgacctg caccagggca tcgtgtgggg aacccagacc gaagagacca ggctggacga
    10021 gcgcctcata aaccgcttcg actacgacgg cgactacggc accgtcctca accgcttcct
    10081 gatgcaggcc gccgtaggct atccgctcac cgtccacggc acgggcggcc agacccgcgc
    10141 tttcatccac atccaggaca ccgtgcgctg catacagctc gccctggaga acccgcccga
    10201 gaggggcgac cgggtcaacg tctacaacca gatgaccgag actcaccgtt tgcgcgacct
    10261 tgccaggctg gtctccgagc gcacgggcgc agagatagat tacttgcaga acccacgcaa
    10321 cgaggccgcc gagaacgatc tgcacgtccg ccacgatcgc ttcctggatc tgggattgca
    10381 accgaccact ttgagcgaag gactgctgga ggaggtcacc gagatagccg gtaggtacgc
    10441 ccaccgctgc gacacctcga agatcccagc cacctcgctg tggaggccac aggtggagcg
    10501 tgtacacaag gctcccgcgg agccctccac gctgtgagcc tcgccgccgg acaggtgagc
    10561 atcgcgggcc tcgtcatcgc cgcgctcttc gcggggtacg gcacctacag gtaccgcgcg
    10621 ggggggagca ggctcgatct gctcctctcg ctgctcatag ccttgggcgt cgtcctggtc
    10681 tcggtcgtac cccagatctt cgggatcttt acccgcctgc tcgggctgga ggaccgcggt
    10741 tttgcgctgc tctccatcgc cgtgctgctc ctcttcgggc tcttcctgaa cctccttgg
//
LOCUS       DATMVF010000236         4676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_311396, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000236 DATMVF010000000
VERSION     DATMVF010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311396"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VN178_12110"
     CDS             complement(<1..708)
                     /locus_tag="VN178_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81758.1"
                     /translation="MSHITTGTHHDSPGGAPASRRGLRRIGAVLAGSIVLAALLLILA
                     NLAFAQSAKKKKVQPDLQPFLVEDIYPGETGSEPNWLMDFKGTLLFAANHPRFAEELW
                     RSNGTKPGTRLVKDIDPGPLVIKKMENTETGSSAPDSVLRTKKWIYFQANTVKYGEEL
                     WKSDGTEWGTKVVKDIVPGQGDSGPEDIVSTGPRTTFFRAWDKKHGEELWKTDGTEKG
                     TTLVKDINTDLPPGERCD"
     gene            complement(759..2162)
                     /locus_tag="VN178_12115"
     CDS             complement(759..2162)
                     /locus_tag="VN178_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWS81759.1"
                     /translation="MSRRVIVIGLDSAPPSLLFGRMLEQMPNLAGMVEGGVHGPLRSC
                     DPPITVPAWQVMSTSASPGRLGLYGFRHRRGYSYTEGWTPNSYSVRLPRIWDVLARHG
                     KRSCLVGVPPGYPPKPINGWSVSCFLTPPNAEQYTYPAQLRGEIEKLVGTYLFDVKFR
                     IEDREILREQLFEMTEKRFDVVHHLAATKPWDFFMMVEIGVDRLGHAFWKFFDPEHPR
                     YEPGNPYEGVADEYYRLIDRRLGELLETLEDAVFLIVSDHGSKAMHGAFCINEWLIER
                     GYLKLEERPESIVALDKAAVDWGATRAWGWGGYYARIFLNVEGREGQGVIPAGRYEEE
                     REELAEELRTITRPDGARMRVEVFRPEEIYGAALGNPPDLMVYFDDLDWRSAGTVGHD
                     GFYLSENDTGPDDSVHAMDGIFVLYDPERRCAHSSVEGAGILDVAPTVLELMGVPAPA
                     GMEGRPIELISKEEVFP"
     gene            complement(2173..3936)
                     /locus_tag="VN178_12120"
     CDS             complement(2173..3936)
                     /locus_tag="VN178_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWS81760.1"
                     /translation="MPHAILILVDALRADHLGCYAGEDLGTPNMDRLARQGVVFENAI
                     SQGSWTRPSTASMMTGLYPSQNGVGTRWSKTSEGRLSARTLDPSIPTLAEILTANGHT
                     TAFMGGSAVLKPLFGVTRGFTHNLWRSIANDGAVIVEDFEQWIQTERPESSFCYMHFM
                     DVHKPLPMETIPARLDTGLDLDLVKESKNELMSYYAAAVRRVDQYIGGVIRALESEGM
                     LEDTLIILSADHGEELGEHGGMLSHGRTLYRELLHVPLVMRLPGQAFARERIDQPVQL
                     IDFMPTILDYLQCPPIDVPGRSLLTLIRGEEAERAAAFSEHVKPDRYSQSVTTRTHQL
                     IQSYRLEDSRPGSPADLLPGLSVAAKGQRIQGGRFIPTKIFIQGEERPTLSGMVERVD
                     TASNSVSLMGITCQVNEDTRFIGKEDSGPLTFDGLAVGDRVTLNFTAESNGRRVATTI
                     KRKPPGGKSKIEGRIERVQALEGGLRSVTVLGMDVLIDSNTKIRAMKDENREEITVSP
                     VNRVLTGDFIDVHTELYDVTSDPAETRDIVDERPDIAQELEEMLALWTQSLLGQVQES
                     TGSVDVDPETMEQLRLMGYVE"
     gene            complement(3957..>4676)
                     /locus_tag="VN178_12125"
     CDS             complement(3957..>4676)
                     /locus_tag="VN178_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS81761.1"
                     /translation="VSFGYDPEQPVLRDIELEIQPGEVAAVVGATGAGKSTLSSLILR
                     LYDPVKGSVLIDGRDVRDYKPDSLVEQIGVVLQESLLFQTTIRENIAYGNPKATFEEI
                     QEAARLAYCEDFIRNLPKGFDTVVGERGSTLSGGQRQRIAIARAVIRDVPILILDEPT
                     TGLDAESEEIVMRALERLMKGRTTLMIAHKLSTVRRADRIYVLEGGEIAEEGTHEELT
                     ARGGAYERAFRLQSIPGQDVG"
BASE COUNT          803 a         1507 c         1395 g          971 t
ORIGIN      
        1 gtcgcagcgc tctccaggag gcaagtcggt gtttatgtcc ttgacgagcg tggtgccttt
       61 ctcggtcccg tcggttttcc acagctcctc gccgtgcttc ttgtcccacg ccctgaagaa
      121 agtcgttctc gggcctgtgg acacgatgtc ttcgggacct gaatcgccct ggcctgggac
      181 gatgtccttg acaaccttgg tgccccactc ggttccgtcg ctcttccaca gctcctcgcc
      241 gtacttgact gtgttggcct ggaagtagat ccacttttta gtgcgcaata cactgtcagg
      301 cgccgacgaa ccagtctccg tgttctccat cttctttatt accaggggac cggggtctat
      361 gtccttgacg agcctcgtac ctggcttcgt accgttgctc ctccacagtt cctctgcgaa
      421 cctcggatga ttggctgcga acaacagcgt ccccttgaag tccatcagcc aattgggctc
      481 cgagccggtc tcgccggggt agatgtcctc gaccaaaaag ggctgaaggt cgggctggac
      541 cttcttcttc ttggcggact gcgcgaaggc caggttcgcc agaataagca gcagggcagc
      601 aagcacgatg ctgccagcca gcaccgcgcc gatacgtcgc agcccccgcc gggaggccgg
      661 tgctccgccg ggcgagtcgt ggtgcgtgcc ggtagtgatg tgactcaact agaagctcct
      721 tgtgtcggtg tggtgagacg gggcactatt tcgtacgatc atgggaagac ttcctctttg
      781 ctgatcagct cgatggggcg gccttccata cctgcgggcg cagggacgcc catcaactcc
      841 aggacagtcg gggctacgtc caggataccc gccccctcca cgctgctgtg agcgcatcgc
      901 cgctccgggt cgtagaggac gaagatgccg tccatcgcgt gtaccgagtc gtcaggaccg
      961 gtgtcgttct cggagaggta gaagccatcg tgtccgacgg tccccgccga gcgccaatca
     1021 agatcgtcga agtagaccat caggtcggga gggttgccca atgcagcgcc gtagatctcc
     1081 tcgggccgga atacttctac gcgcatccgc gccccgtccg ggcgcgttat cgtgcgaagc
     1141 tcctcggcca gctcctcgcg ctcctcctcg tagcggccgg cggggatcac accctgcccc
     1201 tcacgcccct ccacgttgag gaagatgcga gcgtagtagc cgccccagcc ccaggccctc
     1261 gtcgcgcccc aatccacggc ggctttgtcg agcgcgacga tgctctcggg tctttcctcg
     1321 agcttcaggt agcctcgctc gataagccac tcattgatgc agaaggcacc gtgcatcgcc
     1381 ttcgagccat ggtcagagac gataaggaaa acggcgtctt caagcgtctc caggagctcc
     1441 cctagccggc ggtctataag cctgtagtac tcgtcggcta cgccctcgta gggattgccg
     1501 ggctcgtagc gcgggtgctc ggggtcgaag aacttccaga aggcgtgacc caggcggtcc
     1561 accccgatct ccaccatcat gaagaagtcc caaggctttg tggcggccag atgatgtacg
     1621 acatcgaaac gcttctcggt catctcgaag agttgttcgc ggaggatctc gcggtcctca
     1681 atgcggaact ttacgtcgaa gaggtaagtg ccaaccaact tttcgatctc gcctctcaac
     1741 tgggcggggt aggtgtactg ctcggcattt ggcggcgtga ggaagcacga gacgctccag
     1801 ccgttaatcg gtttgggagg atagcctggc ggcacgccga ccagacacga ccgcttgccg
     1861 tgacgagcca gcacatccca gattctgggc aggcggacgg agtaggagtt gggggtccac
     1921 ccctcggtgt aggaataccc tctgcggtgg cgaaagccgt agagtcccaa ccggccggga
     1981 ctcgcgctcg tggacatcac ctgccaggcg gggaccgtga taggaggatc gcagctgcgc
     2041 aacgggccgt ggacgccgcc ctcgaccatc ccggcgaggt tgggcatctg ctccagcatc
     2101 ctcccaaaga gcaagctggg cggggcggag tccagtccta tgacgattac ccggcgagac
     2161 atgccagcct tctcattcga catagcccat gaggcggagc tgctccatcg tctccggatc
     2221 gacgtccaca ctgccggtac tctcttgtac ctgccccagg agcgactggg tccagagagc
     2281 gagcatctcc tccagctcct gggcgatgtc cggccgttca tccacgatat cccgggtctc
     2341 ggcggggtcg ctcgtaacgt cgtagagttc tgtgtgcacg tctataaaat ctcccgtgag
     2401 cacccgattc acaggactga ccgttatctc ctctcgattt tcgtccttca tggcccgaat
     2461 cttcgtgttg ctgtcgatca agacatccat cccgagcacc gttacggatc gcagcccgcc
     2521 ttctagggcc tgcacccgct cgattcgtcc ctcgatcttg gattttcctc ctggcggctt
     2581 ccgcttgatc gtggtggcta cacgccgacc attggactca gcggtgaagt tcagcgtgac
     2641 ccgatcgcca accgccagcc cgtcaaaggt caagggccca ctgtcctcct tacctatgaa
     2701 cctggtgtct tcgttgacct ggcaggtaat gcccatcagc gaaaccgagt tgcttgcagt
     2761 atctacccgc tccaccatcc cgctcagcgt cggtcgctct tccccctgta tgaatatttt
     2821 ggtcggtatg aagcgccccc cctggatccg ttgtcccttg gccgccacgg aaagaccggg
     2881 tagcagatcc gccggggagc ccgggcggga atcttcaagc cgataacttt ggataagctg
     2941 gtgcgtgcgg gtcgtcaccg actggctgta tcggtccggt ttcacgtgct cgctgaaggc
     3001 ggcggctctc tccgcctcct cacccctgat caaagtcaga agactccgtc ccggcacgtc
     3061 tatgggcggg cactgcagat agtcgaggat ggtgggcatg aagtctatca actgaaccgg
     3121 ttggtcgatc ctctcgcggg cgaaagcctg tccgggcaac cgcatgacaa gcggtacgtg
     3181 caagagttcc ctgtagaggg tacgtccgtg ggagagcatt ccgccgtgct cccccaactc
     3241 ctcgccatgg tcggcgctga ggatgatcag ggtgtcctct agcattccct cactctccag
     3301 ggcgcgaata acccccccga tatactgatc tacccgtcgg acggcggccg cgtagtagct
     3361 catgagttcg ttcttgctct ctttgactag atcgaggtcc agaccggtgt ccagccgggc
     3421 ggggatcgtc tccatcggca agggtttgtg tacgtccatg aagtgcatgt agcagaagga
     3481 gctttctggg cgctcggtct gtatccactg ctcgaagtcc tcgacgatga cggcgccgtc
     3541 gttggcgatg gagcgccaca gattgtgggt gaagcctcgg gtgacgccga acagaggctt
     3601 caggactgcg ctgccgccca taaaggcggt ggtgtgcccg ttggcggtca ggatctcggc
     3661 cagcgtgggg atggaaggat cgagggtgcg ggcggagagc ctcccttcgc tcgtcttgct
     3721 ccagcgagta ccgaccccgt tctggctggg gtagagaccc gtcatcatgg atgcagtcga
     3781 tggcctggtc caagacccct gactgatcgc gttctcgaat acgacgccct gccgcgccag
     3841 ccggtccatg ttgggggtgc ccaggtcctc gccagcgtag cagccgaggt gatctgcccg
     3901 tagtgcgtct accaggatca atattgcgtg aggcatctct taaaaccctc ctgtaatcat
     3961 ccaacgtcct gaccggggat cgactgcagg cggaacgcgc gctcgtacgc tcctccgcgc
     4021 gccgtcagtt cctcgtgcgt cccctcctcc gcgatctccc cgccttcgag gacgtagatc
     4081 ctgtccgccc gcctcacggt cgagagcttg tgggcgatca tcagcgtcgt ccgtcccttc
     4141 atcagccgct ccagcgcccg cattacgatc tcctcggact cggcgtcgag cccggtcgtc
     4201 ggctcatcca ggatgagtat cgggacgtcc cggatcacgg cgcgggcgat ggcgatacgc
     4261 tgccgctgtc cccccgacag cgtgctcccc cgctccccga ccacggtgtc gaaccctttc
     4321 ggcaggtttc ggatgaagtc ctcgcagtag gcgaggcggg cggcctcctg aatctcctcg
     4381 aaggtcgctt tgggattgcc gtacgcgatg ttctcccgga tggtcgtttg gaagagtagt
     4441 gactcctgga ggaccacccc gatctgctcg accagcgaat ctggcttgta gtcgcgaacg
     4501 tcgcgcccgt ctatgagcac ggagcccttc acggggtcgt agaggcgcag gatcaggctc
     4561 gacagagtgc tcttccccgc gccggtggcc cccacgaccg ccgccacctc gcccggctgt
     4621 atctccaact cgatgtcccg cagcaccggc tgttccgggt cgtaaccgaa ggagac
//
LOCUS       DATMVF010000237        11382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_26009, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000237 DATMVF010000000
VERSION     DATMVF010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26009"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..928)
                     /locus_tag="VN178_12130"
     CDS             complement(11..928)
                     /locus_tag="VN178_12130"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWS81762.1"
                     /translation="MELRQVRTFRVVAEELSFSRAAARLGYVQSSVSAQVAALERELG
                     VRLFDRLGRRISLTEAGSVMLAYSRELLVLAGEAREAVIDAGAGEGEVTGSLTVSAPE
                     TLLTYRLPRLLAIFHERHPKVRLSVRPSAIGRLVGSMRRAVDEGRVDVAFVLDGTVVV
                     PGLSVEPLIEEGVTVIAPSDHALASSTSVVPQDVTGETMLLPEAPESGCAYRGQFERQ
                     LGESGVVPPERMEFQSIEAVKQCVAAGMGISVLISAAVEVEIEDGQLAALRWEEPFEV
                     FTQMVWHEKRWKSPALRAFLETAREVFVR"
     gene            1053..2294
                     /locus_tag="VN178_12135"
     CDS             1053..2294
                     /locus_tag="VN178_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81763.1"
                     /translation="MARDEGEVRGELRSGLPAVVLLLLGTLGISTAFGMLLLLPLYVQ
                     ELGGDEADFGVVLSAAAVPAVLCIGLLIRYPEALRPHVVVALAIATYGIGAVGASLVT
                     GGWTPLVGVGVLLGTAWAVVYAATPMVMSAMVTDEGRAAYFGYLTGTQQVGIGVGPVI
                     SRFLVETPLGFRGTFLVAGALCLVSMALTFVVGALVSDAGTDAGHGGAEGNVASTVSF
                     GAAMRRILVSEAAFSLAMILLFACLFTTMTSFQPTFAGERGLDYSVFYVTYTVAVIFS
                     RFVLAGLAARFDSRLVIAGAVSVMALAVASFLVVDSNTLFYGISSAFLGLGYGLALPS
                     VQAQAVNVSEEQVRPRVLPMAGMLFEGAILGFPLVAGWVITGFGYRTLFAVLLAFALV
                     QVAIAWWRFLVAGRSKGTQEV"
     gene            2519..2797
                     /locus_tag="VN178_12140"
     CDS             2519..2797
                     /locus_tag="VN178_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81764.1"
                     /translation="MREDQSVAEMANEVLMRQARIRSERSGEPIEVAMEAVLNTEAGK
                     QLRELRDGPHAEERVEEAQVGAARERAQKRVEDLGKLLAEYPEVPTHG"
     gene            2976..3230
                     /locus_tag="VN178_12145"
     CDS             2976..3230
                     /locus_tag="VN178_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81765.1"
                     /translation="MKRMIKVLLVAVLMAVILVASISPAMAVRRAGVLLSSPQPCLAN
                     TQNPNLVLNPPGRTPGCWFLLPPGAASNPPVTIDSGGVVL"
     gene            complement(3291..6095)
                     /locus_tag="VN178_12150"
     CDS             complement(3291..6095)
                     /locus_tag="VN178_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS81766.1"
                     /translation="MESRTRRTAQKLMADQPTGTVTLLFTDIEGSTKLWEENPRGMQV
                     ALTRHDALLWEAIEGHGGFVFKTVGDAFCAVFPTALGALEAALAAQRSLFSEAWGEEI
                     GALKARMALHTGTTHERDGDYFGPPVNRVARLLSAGHGGQVLLSSSTQDLVRDHLPPH
                     THLRDLGERRLKDLSRPERVFQLTAPDLPSEFPPLRTLESRTNNLPLQATPLIGRERE
                     VEAACGLLRNSETRLLTLLGPGGTGKTRVGLQVAAELADDFEDGVFFVPISAITDPAL
                     VAPTIARTLGLSEGAQPPEELLEGYLRDRQTLLLLDNLEQVIEAAPVVERLLSGAANL
                     KILATSRIPLGLYGEYEFPVPPLSLPDPDSLPPLEHLTEYEAIRLFVERARAVRPDFS
                     LTEENGPAVVEICKRLDGLPLAIELAAARIKLLPSRVLLDKLGNRLKILTGGARNLPE
                     RQRTLRNAIEWCYELLDEGEKLLFGRLGVFSGGATLEAIEAVCDAEGDLPTDVFDGAS
                     SLLDKSLLRQEEGAAGEPRFAMLETIHEFANVKLAESGEVEAVRRAHAEYFLILAEVA
                     EPMLWGAEDAAWLDRLEREHGNMRAALSWAIEHDEGELALRLGGALRWFWYMEGYYGE
                     GRRWLEATLGKDWRAAAAEARAKALEGVGWLASGQGDLDRAQAAAEEGLKLSAEAVLG
                     DVVVADLQNVLGEAVARQRGDYEWAAKLLTESLALHRKAGDIRGIAWSLCNLANVTSD
                     RGDYEQAKELYEEGLALSRELDGAELLGAYLINLGYESLLEGEPERAIALNEEATELF
                     RKRGRRGGLQHTLGNLGWATLLRGDFERAETLHKQSLRVSQDLGDKLVASDSVEGLAC
                     TAGARGEAERSAILFGAAEGQREAEGYEQAPRDRSLREPYLAAAHAQLGEAAWSAAWE
                     KGRSMQLEDTIGYALEDSNG"
     gene            complement(6363..7214)
                     /locus_tag="VN178_12155"
     CDS             complement(6363..7214)
                     /locus_tag="VN178_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS81767.1"
                     /translation="MSDDPTTGAAPTVNEGDRLQRSGEAGLRRLTFLGTGDALNGERA
                     QSSLALPLSGGETMLIDTSSGTVLLGRLEAAGIPLENVRHLFLSHRHFDHVGGFAPLI
                     AALASLPEASHVVHAAPDTLRALQNLLDLTLPGAGGWLGERLGWRELIPGKPTEVGET
                     VVTPFSLDHGLECVGFRIAQPGAVVAFSIDTRPTREIAENASGADLLVHDAYGPKDAA
                     EQAHAMGHSTATDAGEAARAAGVRRLVLTHLRSSRFVDPEALAAEAETAFGGPVRAAQ
                     DLDTIEF"
     gene            7438..8235
                     /locus_tag="VN178_12160"
     CDS             7438..8235
                     /locus_tag="VN178_12160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HWS81768.1"
                     /translation="MAFDLGAFDLDGTVLRRNLRITQGTVAALQRLRERGMRLVVATG
                     RRFESAREYAGRLGFADRDPLICYGGSMVRRLNGETLLHRTLPGSLGIEVLEWAAARD
                     LHARVFLDGRIITPPDTPVARPESDASIVDSPADWLRDSGEEPTKLVIVDRPDDIQGW
                     LGEAQTVFAGRLFVTRSLPHYVEIGSLQGTKASALGFLCERWGIEPERVLAFGDADND
                     IDMLRFAGHGVAVGGMTGEVREAADRVVPGVDDDGVAGYVETLLGGP"
     gene            8237..9064
                     /gene="mutM"
                     /locus_tag="VN178_12165"
     CDS             8237..9064
                     /gene="mutM"
                     /locus_tag="VN178_12165"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWS81769.1"
                     /translation="MPELPEVTVISEDVAALAVDREVLRAAVFRPDVTNVEPQEFGRR
                     LVGRTLRATGRRGKIIVLDFGEIVGLVHLVISGRVLRLPEWTKPDRMNTAILEFEGEG
                     EPLVLAFTRLWLGYFDLYEPGEEERHPLISRLGPDPLSEDFTVEYLASAFSRRASVKA
                     LLLDQSVVAGLGNIYVDEILFSARVHPTRKANTLTTSELQSIHAATRDILRRAIELRG
                     TTFDSYHDAFGETGKFQHQLQVFIRTGEPCPRCGTKIVKARVAGRGTHTCPTCQMAS"
     gene            complement(9333..9911)
                     /locus_tag="VN178_12170"
     CDS             complement(9333..9911)
                     /locus_tag="VN178_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81770.1"
                     /translation="MRNMIYRLAPVVVILLSAFVLAGCSVVFGEDPREQADQAISEAN
                     QSIAQHNQLFDAARSTYADVKQKIESGEDPSKEKDNITEAKNTLERARQDLQDARESL
                     QTVADLDVDPSVKEYAGLLSKAMDAQLAAEAKEIQFYGILEEDPTLQNNREEALALLS
                     EVGEGYQRAEQTYDEAQNLANSNPELIEAAPE"
     gene            9979..10311
                     /locus_tag="VN178_12175"
     CDS             9979..10311
                     /locus_tag="VN178_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81771.1"
                     /translation="MLARLLGRRKREPDLAMISLAASSIREYAGKHAALFERAERLQE
                     KADRLEKAGTPSESAANRAERARKEVEAGLRALRASFASSVGGSAGERAFDHEVERLY
                     PTLGVRGQ"
     gene            complement(10351..10809)
                     /locus_tag="VN178_12180"
     CDS             complement(10351..10809)
                     /locus_tag="VN178_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81772.1"
                     /translation="MFGRKSRAERLKEQAESNSLVPMATLAAVYTGAMPVLERLLYDD
                     DLRDNIRTFIESARSIVDQLSGEDPADIITKLWDDDKLRGQVEAAAGAASQGSKRIRG
                     EKVRERGGWGGRLLFLLLVAAVGFLFLHPRTGPQARKFAVDTYGSLTSGE"
     gene            complement(10877..>11382)
                     /locus_tag="VN178_12185"
     CDS             complement(10877..>11382)
                     /locus_tag="VN178_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HWS81773.1"
                     /translation="RYGRLVPGLRAALAEAAPEVGVRAAAGESISVDVEGEEITLSPE
                     ELLVEPGQREGYALEREGGLSVALRTGLDAGLVDEGLVRELVHRMQNLRREKGFEIEE
                     SISVGLFGNPRVSSLLEGRWGDYFKSEVLAKELSIDSGAPGEGSERVTVDGEALWVRM
                     EPLGRAG"
BASE COUNT         2001 a         3684 c         3507 g         2190 t
ORIGIN      
        1 ttcccgacta ctatcttacg aagacctctc gggccgtctc caggaacgct ctcagggccg
       61 gggacttcca ccgtttctcg tgccacacca tctgggtgaa gacctcgaaa ggctcctccc
      121 accgcagtgc ggctagttgg ccgtcctcga tctcgacctc cacggccgcg ctgatcagga
      181 cagaaatgcc catgccggcg gccacacact gctttacggc ctctatgctc tggaattcca
      241 tccgctccgg cggcacaaca ccactctccc ccagctggcg ctcgaactgg ccccggtagg
      301 cgcaacctga ctccggagct tcgggcaata gcatcgtctc accggtcaca tcctgcggaa
      361 ccaccgacgt cgacgaagcc agcgcgtggt cggaaggagc gatgacggtc acaccctcct
      421 ctatcaaggg ttctaccgat agcccgggaa ccacaacggt cccatcgagc acgaatgcga
      481 cgtccaccct gccctcatcg acagcccggc gcatgctacc caccaaccgg ccgatagcgc
      541 tcgggcgcac cgagagcctg accttgggat gcctctcatg gaagatggcc aggagcctcg
      601 gcaacctgta ggtaaggagc gtctcggggg cgctcaccgt gagagatccc gtcacttcgc
      661 cctcgcccgc accagcgtcg ataaccgcct cccgcgcctc tcccgcgaga accagcaact
      721 cccgggagta cgcgagcatc acagatcccg cctccgtgag cgagatcctc cgccccaacc
      781 ggtcgaataa ccgcaccccg agctcccgct ccagcgccgc aacctgggcg ctcacactcg
      841 actgaacgta acccaacctc gccgccgccc gcgagaacga aagctcctcc gccaccaccc
      901 ggaaagtcct gacctgcctg agctccaaga gatccccctt actgccgact tatcggatac
      961 atcgatactt caaatcatta tatttcgttg gacacgatat tagcacggta ttaggatctt
     1021 ctcgaacgac acgaagaaca gatggagaag acgtggcgcg agacgaaggc gaagtacgag
     1081 gggaattgcg gagcggtctc cccgctgtgg tgttgttatt gctgggaacg ctcgggatct
     1141 cgacggcttt cgggatgctc ttgctgttgc ccttgtacgt tcaagagctt gggggtgacg
     1201 aggcggattt cggcgtggtc ctgtctgccg cggcggtgcc tgcggtcctc tgcattgggc
     1261 tcctgatccg ctaccccgag gccttgaggc cgcacgtggt ggtggcgcta gccatagcga
     1321 cctacgggat cggggccgtc ggcgcatcgc tcgttaccgg aggctggaca ccgctcgtcg
     1381 gggtcggggt cctgctcggc accgcctggg ctgtggtcta cgcggccacg ccgatggtca
     1441 tgagcgcgat ggtaaccgac gaggggaggg cagcgtactt cggctacctc acgggcaccc
     1501 agcaggtcgg aatcggcgtc ggaccagtga tctcccgttt cctggtcgag acacctctgg
     1561 gcttccgtgg gacgttcctc gtggccggcg ccctctgcct cgtctccatg gcgcttacgt
     1621 tcgtcgtcgg tgcgctcgtc tctgacgccg ggacggatgc cggacatggg ggtgcggaag
     1681 gaaacgtggc ctccacggtc tcgtttggcg cggcgatgcg gcgtattttg gtctccgagg
     1741 ccgccttctc gctcgcgatg atcctgcttt tcgcctgcct cttcaccacg atgacgagct
     1801 tccagcctac cttcgccggc gagagagggc tcgactactc cgtcttctac gtcacctaca
     1861 cagtagcggt gatcttctcc cgcttcgtgc tcgcaggact cgcggcgcgc ttcgattcgc
     1921 ggctcgtgat cgccggggcg gtaagcgtca tggcgctcgc ggtagcgtcg ttcctagtgg
     1981 tggattccaa caccctcttc tacgggattt cctcagcatt cttgggcttg ggctacgggc
     2041 tcgcgctgcc gagcgtgcag gcgcaggcgg tcaacgtctc ggaggagcag gtaaggccgc
     2101 gggtgcttcc gatggcgggc atgctcttcg aaggtgcgat cctcggcttc ccgctggtcg
     2161 ccggatgggt cataacgggt ttcggataca ggaccctgtt tgcggttctc cttgctttcg
     2221 cgcttgtaca ggtcgccatc gcctggtggc gcttcctggt cgccggtagg tcgaagggta
     2281 cgcaggaagt gtaagaaccc taccttacgg tcctcatctt tgtgctgatc tacagcaccg
     2341 tgcataaagg ttggccctca tgaacgacgg gctatcagcg ctcgactccc agctttcagc
     2401 ccttgccctg gctgaaagct gaaggctcca gtttcttgac tacggcccgt tagccaggag
     2461 aagccgatca aggttatact caccccgggc gaaagttttg cgcaaggaga ggagcaccat
     2521 gcgcgaggat caaagcgtcg ccgagatggc gaacgaagtg ttgatgcgac aagctcggat
     2581 ccggtcggag cggagtggtg agcccatcga agtagcgatg gaggccgttc tgaacaccga
     2641 ggccggcaag caactcaggg aactcaggga cggcccccat gccgaggaga gagtcgaaga
     2701 ggcgcaggtg ggcgcagcaa gggagcgagc acagaagcga gtggaagatc tcggcaagct
     2761 actcgcggag taccctgagg tccccacgca cggttaacgg gacgagcgag cggggaacgg
     2821 ccttgtgagc acggtcttgc gtctccggaa ggagcgatca gaaggttgcc aagaactgga
     2881 gaaatagttc atccatacga taccctcggc agcttgggtc tgttattgtg agcgcagaga
     2941 agccggaaag gtcagtgcaa agaggaggtt ggtcgatgaa acggatgatc aaagtcctgt
     3001 tggtggcggt gctcatggcg gtgatcctgg tagccagtat ctctcccgcg atggccgttc
     3061 gcagagcggg cgtgttattg tcgtcgcccc agccctgcct cgcgaacacc caaaacccga
     3121 acctggtcct caatccacct ggtcggacgc cggggtgctg gtttttgttg ccgccaggtg
     3181 ccgcaagtaa cccaccggta acgatcgact cggggggcgt cgtactctga gtgctacatt
     3241 cgttcgggtg ccgggaggga gctctctaga aggctgagcc ggcggacaga tcagccgtta
     3301 gaatcttcga gggcgtagcc tatcgtgtct tcgagctgca tggaacgccc cttctcccac
     3361 gccgcagacc aggccgcctc gcctaactga gcgtgggcgg ctgccaggta cggctctcgt
     3421 agggagcgat ctctgggagc ttgctcgtag ccttctgcct cacgctgtcc ttctgcagcc
     3481 ccgaacagta tcgccgaccg ctcggcttct cctcgagccc cagcagtgca tgccaatccc
     3541 tccacgctat ctgaggcgac tagcttgtcg cccagatcct gggataccct taggctttgt
     3601 ttgtgcaagg tttcggcccg ttcgaagtca cctcgcagga gcgttgccca tccgaggttg
     3661 ccgagagtgt gttgcagacc gcccctacgc cctcgcttcc gaaataactc cgtggcctcc
     3721 tcatttagcg ctatcgccct ctcaggttcg ccttcgagca gggactcgta gcccaagttg
     3781 atcaagtaag cgccgagcag ttccgcaccg tccaattctc gggacagggc caagccctct
     3841 tcatagagtt ccttcgcctg ctcgtagtcg ccgcggtcgc tcgtcacgtt ggccaggttg
     3901 cacagggacc acgcgatgcc tctaatgtcc ccggctttgc ggtgcagcgc cagactttct
     3961 gttagcagct tcgccgccca ctcgtagtcg ccccgttgtc tcgcgacggc ttcccctagc
     4021 acattctgta ggtcagctac gactacgtct cccagcactg cctcagcgct cagcttaagc
     4081 ccctcttcgg cagcggcttg cgcccgatca aggtcacctt gccccgacgc cagccagcct
     4141 accccctcca gagctttggc acgggcctca gctgccgccg cgcgccagtc cttgcccagc
     4201 gttgcctcga gccatctcct tccctcaccg tagtatcctt ccatgtacca gaaccagcgc
     4261 aacgctcccc ctagtctcag ggcaagttcg ccctcatcat gctctatggc ccaggagagt
     4321 gctgccctca tgttgccgtg ctctcgttcc agccggtcca accacgccgc atcctctgct
     4381 ccccacaaca tgggctcagc cacttccgcg aggatcagga agtactcagc atgggcacgc
     4441 cttacggctt ctacctcccc gctctccgcg agttttacgt tcgcgaactc atggatggtc
     4501 tcgagcatcg cgaagcgcgg ttcgcccgct gctccttcct cctgacgcag caggctcttg
     4561 tcgaggagcg aggaggcgcc atcgaatacg tccgtgggga gatctccttc agcgtcgcac
     4621 accgcctcga tggcctccag ggtggcccca cccgagaaga ctcccagcct cccgaagagc
     4681 agcttttcgc cctcgtcgag tagctcgtaa caccattcga tagcgttcct tagggttctc
     4741 tgccgctcgg gcaggtttcg cgcgccaccg gtgaggattt tgagccggtt gcctagcttg
     4801 tctagcagca cccgcgaggg gagcagcttt atgcgtgcag cggcgagctc tatggccaag
     4861 ggtagcccat cgagcctctt gcagatctcc accaccgcag gtccattctc ctccgtcagg
     4921 gagaagtctg gccttacggc cctggcccgc tccacgaaga gcctgatcgc ctcgtactcg
     4981 gtgaggtgtt ccagaggtgg cagggagtcg ggatcgggca acgagagagg aggtactggg
     5041 aactcatact caccgtagag cccgagcggg atgcggctgg tggccaggat cttcaggttc
     5101 gctgcgcccg agagcagcct ctccacgacc ggcgccgctt ctatgacctg ctcgaggttg
     5161 tcgagcaaca ggagcgtctg tctgtcgcgc aggtagccct cgagcaactc ctccggtggc
     5221 tgggcgcctt cactcagccc cagtgtcctt gctatggtcg gggccacgag cgcgggatcc
     5281 gtgatggcgg agatcggcac gaagaaaacc ccgtcttcga agtcgtcggc aagttctgct
     5341 gctacctgca gtcccacacg ggtcttcccc gtgcctccgg gtcccaagag ggtcaagagc
     5401 cgtgtctcgg aattgcgcaa cagcccacac gccgcttcca cctcccgctc acgcccgatg
     5461 agtggtgtag cctgcaaggg caagttattg gtgcgagact ccagggttct caaggggggg
     5521 aactctgacg gcaggtcggg agccgtaagc tggaagaccc tctctggtct ggagagatcc
     5581 ttgaggcgtc tctcccccag gtccctgaga tgtgtgtgag gcggcaagtg atccctcacc
     5641 aggtcctgcg tcgaggaaga aagtaagacc tgcccgccgt gaccggccga gagcagcctc
     5701 gctacccggt tgaccggagg gccgaagtag tctccgtccc tttcgtgggt ggttcctgtg
     5761 tgcagagcca tccgcgcctt gagggcaccg atctcctctc cccatgcttc cgagaagaga
     5821 ctcctctggg ccgcaagcgc cgcctcgagg gcaccgagag cggtggggaa gaccgcgcag
     5881 aaagcgtcgc ccaccgtctt gaagacgaaa ccaccgtgcc cttctatggc ctcccagagc
     5941 agggcgtcgt gacgggtcag ggcgacctgc atgcctctag ggttctcctc ccacagcttc
     6001 gtcgaaccct ctatgtccgt aaagagtaag gtgacggtgc cggtgggctg gtcggccata
     6061 agcttctgag cagtcctcct tgtcctggac tccacttata tagcatcccc gacaacatga
     6121 tgcacagctc tctgacgact tggaacagcg tttcagcacg tcgcctgttc caggcgactt
     6181 gtcagcactt cagcaagcct gcttcgcagg cttgtcagca tgtcagcttt tacgtgggat
     6241 caagagcact ggctgacgag gcggcgaagc cgccgtgctg acaagcgaag gcgtcagcct
     6301 gagcgtgctg aattgctgaa atgcgcgggt atgggaaggc tacagccttc ccatacccgc
     6361 cttcagaact cgatcgtgtc gaggtcttgc gccgctctta caggtcctcc gaacgcggtt
     6421 tcggcctcgg ctgcaagcgc ttctggatcg acgaaccggc tcgagcgcag gtgggtgagg
     6481 acgagccgcc gcacgccggc cgcgcgggcc gcctctcctg catcggtcgc cgtcgaatgt
     6541 cccatggcgt gcgcctgctc cgccgcgtcc ttcggaccgt aggcgtcgtg gacgaggaga
     6601 tccgccccac tcgcgttctc ggcgatctcc cgggtcggtc gggtgtctat ggagaaggcc
     6661 acgacggcac caggttgggc gatgcggaag cctacgcact ccaggccgtg gtcgagcgag
     6721 aagggtgtaa ccacggtctc gcctacctcg gtaggcttcc cagggatcag ctcacgccag
     6781 ccgaggcgtt cgccgagcca gccccctgcg ccgggaaggg tcaggtcgag gaggttctgc
     6841 agcgcacgga gcgtgtcggg tgcggcgtga acgacgtgcg aggcttcagg gagggaggcc
     6901 agggcggcga tcaggggagc gaatccgccg acgtggtcga agtggcggtg gctcaagaac
     6961 aggtggcgca cgttctccag cgggatcccg gccgcctcga ggcgaccgag cagcacggtg
     7021 ccgctggagg tatcgatcag catcgtctcc ccaccagaga gcggtagggc caggctggac
     7081 tgcgccctct cgccgttgag ggcgtcgccg gtaccgagga aggtcagacg tctcaagccg
     7141 gcctctcccg agcgttgcag cctatctcct tcgttcaccg tgggagcagc gcccgtcgta
     7201 ggatcatcgc tcatgtacgc cttgcgtata acacaagtag caggcggacg cgagcatatt
     7261 atcaggcaaa tcttgaagcg gttcacgaac cgccctttcc ggcaaaccgc ttccaaagat
     7321 gctacaaact actttactgc ctctacaagg gcagagttca gggacgccaa tccctcagga
     7381 tctacggcat ctgaaaggtt tcacttgggc gcggaacaca gaggtacgaa gaacggattg
     7441 gccttcgatc tcggcgcctt cgacctcgac gggaccgtgt tgcggcggaa cttgaggatt
     7501 acacaaggca ccgttgcagc cttgcagcgt ctcagagagc gggggatgag gctcgtcgtg
     7561 gccacgggga gacgcttcga gagcgccaga gagtacgcag ggaggctcgg ctttgcggat
     7621 cgtgaccccc tgatctgcta cggcggttcg atggtgagga ggctgaacgg ggagaccctg
     7681 ctccaccgca cgctgccagg gagtctgggc atagaggtgc tggagtgggc agcggcgcga
     7741 gatctgcacg cccgtgtctt cctcgatggg cgcatcatca ccccgccgga cactccggtg
     7801 gcgcgccccg agtccgacgc ttccatcgtg gactcgccgg cggattggct cagagacagc
     7861 ggggaagaac cgacgaagct cgtgatcgtg gaccgccccg acgatataca gggttggctc
     7921 ggggaggctc aaacggtttt cgccggacgg ctcttcgtca cgcgcagcct gccgcactac
     7981 gtcgagatcg gcagtctaca aggcaccaag gcgtcggccc tcggcttcct gtgcgagcgg
     8041 tgggggatcg agccggagcg cgtcctcgcc ttcggcgacg ccgacaacga catagacatg
     8101 ctccgcttcg ccgggcacgg ggtcgcggtg gggggtatga ccggcgaggt acgcgaagcc
     8161 gccgataggg tagtacccgg cgtggacgat gacggtgtcg ccggctacgt cgagacgctt
     8221 ctcggaggac cctgagatgc ccgagctgcc cgaggtcacc gtcatctcgg aggatgtggc
     8281 cgcgctcgcg gttgaccgcg aggttctgcg ggccgccgtc ttcaggcccg acgtgaccaa
     8341 cgtagagccc caagagttcg gcaggcggct cgtcggtagg acgctgcgcg cgacgggaag
     8401 aaggggcaag atcatcgtcc tcgacttcgg ggaaatcgtc ggactcgtgc atctggtcat
     8461 ctcgggaagg gtattgcgcc tgccagaatg gacgaaaccg gacaggatga acaccgcgat
     8521 cctcgagttc gagggcgaag gggaaccttt ggtgctggct ttcacgcggc tctggctcgg
     8581 ttacttcgac ctctacgagc cgggggagga agagagacac cccctgatct cacgcctcgg
     8641 ccccgatcct ctctccgagg acttcacagt agagtacctg gcctccgcat tcagtcgcag
     8701 ggccagcgtt aaagccctgc ttctcgacca gtccgtagtg gccggcctcg gcaacatcta
     8761 cgtggacgag atcctcttct cggcccgcgt ccatcccacg cgaaaggcca acaccctcac
     8821 cacaagcgaa ttacagagta tccacgcagc aacccgcgac atcctgcgtc gcgccatcga
     8881 gttgcgcggc accaccttcg actcctacca cgacgccttc ggggagaccg gcaagttcca
     8941 gcaccagctc caggtcttca tccggacagg cgagccatgc ccacgttgcg gcacgaaaat
     9001 cgtaaaggcg cgcgtcgccg gtcgaggaac acacacctgc ccaacttgcc aaatggcaag
     9061 ttagcacgcc gccctgcggg cagctagtca gcacggcggc tgcgccgcct tgtcagcact
     9121 tcagcatttc agcttcttcc gttctggctt cgccctgccg cgcacgacga agcatggatc
     9181 agggaaatag gttcgacgct cgtggcggcg cttcgggacg actagctgaa atgctgacga
     9241 gccgccgagc gaagcgagga ggcgtgctga caagcgacag ccgtaggctg gagcgtgctg
     9301 acaagctgcc cgcagggcgg cgtgctgaaa tgctattctg gcgccgcctc gataagctcg
     9361 gggtttgagt tggccaggtt ctgggcttca tcgtaggttt gctcggctct ctggtagcct
     9421 tccccgactt cggagagcag ggcgagggct tcctcccggt tgttctgcag ggtgggatct
     9481 tcttccagga tgccgtagaa ctgtatctcc ttggcctcgg cggcgagttg ggcgtccatc
     9541 gcctttgaga gcagaccggc gtactccttt accgaagggt cgacgtcgag gtcggccacg
     9601 gtctgtaatg attctctggc gtcctgtaga tcctggcgtg cccgttcgag ggtgttcttg
     9661 gcctcggtga tgttgtcctt ctccttcgaa gggtcttccc cggattcgat cttctgcttg
     9721 acgtcggcgt aggtcgagcg ggcagcgtcg aagagctggt tgtgctgggc gatggattgg
     9781 ttcgcttcgc tgatggcctg gtcggcctgc tcacgggggt cctccccgaa gacgaccgag
     9841 catccggcga gcacgaatgc cgagagcaaa atcacaacga cgggcgccag gcggtatatc
     9901 atgttcctca tgagtgaaag cagtctacct ctcttgaacg cactcaggct acggtccttt
     9961 cccggaggcg ccaggcgcgt gctcgcccgg ctactcggtc gtcgaaaaag ggagccggac
    10021 ctggccatga tctcgctggc ggcatcatcc atccgggagt acgcagggaa gcacgccgcg
    10081 cttttcgagc gggctgagcg gctgcaggag aaagccgacc ggctggagaa ggccgggacc
    10141 ccgagcgaga gtgccgccaa ccgcgcagag cgcgccagaa aagaggtcga agccggactc
    10201 cgcgccctcc gcgcttcttt cgcttcatcc gtgggaggga gtgctggaga gcgggcattc
    10261 gaccacgagg tcgagcgtct ttacccgacg cttggcgtcc ggggccagta actccggcgc
    10321 tcctcgcgaa gcgttcgttc ttttccgctc ctattctcct gaggtcagag acccgtaggt
    10381 gtccacagcg aacttgcgtg cctggggtcc tgtcctcggg tgcaagaaca ggaagcctac
    10441 ggccgctacc aggaggagga acagcaggcg accgccccat ccaccgcgct cgcggacctt
    10501 ctcccccctt atccgcttgc tgccctggct ggccgctccc gccgcagcct cgacctggcc
    10561 gcggagctta tcgtcgtccc acagtttggt gatgatgtcg gcggggtcct cgccggagag
    10621 ctggtccacg atcgagcggg ccgactcgat aaaggtccgg atgttgtccc tgaggtcgtc
    10681 gtcgtagagc agacgctcga gaaccggcat ggcgccggta tagaccgcgg ccagcgttgc
    10741 catcgggact agcgagttgg actctgcctg ctccttgagc ctctccgccc ggctctttct
    10801 gccaaacaca ccgaacctcc ctctgctgaa tcgtctatgc ctgtgacccc tacatcatac
    10861 ccgtttcgcc gggcctctac cctgccctgc cgagcggctc cattctcacc catagagcct
    10921 ccccgtcgac cgttaccctc tcagagcctt caccaggggc gcccgaatcg atggagagct
    10981 cctttgccag tacctcgctc ttgaagtaat ctccccagcg accctccagt agcgacgaga
    11041 ccctggggtt cccaaagagg ccgacgctga tgctctcctc tatctcgaaa cctttctcgc
    11101 gtctcaggtt ctgcatcctg tggaccagct cccgcacgag tccctcgtcg acgagtccgg
    11161 cgtcgagtcc cgtgcggagc gcgacggaga gacctccctc gcgctccagg gcgtaaccct
    11221 ccctctgtcc cggctcgacg agcaactcct cgggagagag cgtgatctcc tccccttcca
    11281 cgtccacgga gatgctctct cccgcggcgg cccggacgcc gacctcgggc gcagcctcag
    11341 cgagggccgc cctgaggccg ggtacgagcc tgccgtacct gg
//
LOCUS       DATMVF010000238        23102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_26300, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000238 DATMVF010000000
VERSION     DATMVF010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 23102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23102
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26300"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VN178_12190"
     CDS             complement(<1..1084)
                     /locus_tag="VN178_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HWS81774.1"
                     /translation="MNTRDRHRLERHARRVEELKLWRNARESRIEEWRFAADGGEVKE
                     IGLGDFWPEEALPASFSAEAKIPEAWAGSPVELELWLGGEGFVSLSTGFSGGLNPFHR
                     SFPVTQKAAGGEKVGVEAEVVPRGMFGSNVAEPQLRRSSLVVPETEVRALERDLGLIV
                     EVCEQLGDHEVVPHLLDVVDTAFAGLFSLWPSGSEVALTRYLEAYAESTDGGPWSLPP
                     APRVVEPLSEETRGAVREAREMVASRLEKLKAEYPPVGRLALTGHAHLDLAWLWPVSE
                     TMRKGRRTFASVLSLMERYADFVFNQSSAQLYRWIETGSPEIFERVRRRVEEGRWEPV
                     GGSWVEPDCQIPSGESMVRQMLYGQRY"
     gene            complement(1223..3493)
                     /locus_tag="VN178_12195"
     CDS             complement(1223..3493)
                     /locus_tag="VN178_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HWS81775.1"
                     /translation="MIVHMIGNAHIDPVWLWPWQAGVDEALATFRSAADRCDEYPEFV
                     FTRGESWLFEQVERIDPGLFERVRRLVASGRWHITGGQYIQPDVNLSTVMGLHRQIIH
                     GQRYFEDRFGVSPKVGYNVDSFGHPATLPDVLAAHGYVGYVFHRPGAHQVELPAQTFR
                     WRGVGGEEVIGFRISPAYVTFSDDLYGQIMLSIEAANPELGHTMCFYGVGNHGGGPTK
                     ANIEYILENTRSFPDTELRFSTPQAFFDAIEQSRDRLPIVTDELQHTFPGCYSVMHDI
                     KQQQQRGEHLLDQCGRLVEGFVDDEEERRGLHERLDGAWDDLLFTEFHDVLAGTSVPA
                     AWDSIRAMQGRARITGEEVAVEATRRWAHRKLPPLDEQQIVIINPDDAPWEGTVETEP
                     FLSFDAWGNRWLSDLEGRPIDFQVVQPAAAAHVERVVFPLTLAGGQYAQVLVRTDKRP
                     STEAPDSDLEASPHLIANGHLRAELNGSGIRRLIAGGGRDLLGEGGFGLHLRRDLTDT
                     WTFHTDRFVEPIETVLHTDGWVVEETGPLRARVRSEGWLGRSSVRWTLTLHRYEPRIH
                     IRVEVNFSERFKLLQMPVHLAALPTRRTDGLPGGHVERTASATEWPVQGWSHVELADR
                     HLALVTGDAYSLSLDGDCWQWTLLRSPKMAWGGGEPVVYAGRDRYTDQGPQTFAFVLC
                     VGGRLEETMLRTLARQQMQPPVVFDRYEGMDRPPWGNNPPRALWLGAVERALADGRMG
                     HVLENEDVDAVRPLFE"
     gene            complement(3584..4429)
                     /locus_tag="VN178_12200"
     CDS             complement(3584..4429)
                     /locus_tag="VN178_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS81776.1"
                     /translation="MRSRKARGRIGDALTYLVFAAALVFFGGPLLWVLSLSIRTPQEV
                     YITSLRLIPESPTLENYVEVLYSAQFSVFLINSLKLSVAGGVGAMVVAAPAAYAFSRL
                     TFRGNSTLLIGVLALQMISPLVIGVPLYRYFARLNILDSHVSTAMVYIAILVPLATWM
                     LKGFFDGIPPALDEAAMVDGCTRFGAFWRVTLPLILPGLTSAFVLTAILAWGEFTIPY
                     ILLSKPSLLPISIGILNFQGNYAQTSQQVVAAGGVLAMLPAIATFVILQRFIVRALMG
                     GAIKG"
     gene            complement(4426..5331)
                     /locus_tag="VN178_12205"
     CDS             complement(4426..5331)
                     /locus_tag="VN178_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS81777.1"
                     /translation="MTKKAFAPRQLIGENRPTLWMAPLLAVVTLFYLYPAFEVLRFSI
                     TDASLLEPNYDYTLNTFKNVLSDPVLYKVLRITAIFVAASVVLQLTLGLLIAVAVNRA
                     RRRRLPGMVFMRSIILSAWVMPGVVIGIVWAIILNEASYGLANLVLAPVGLGGKAWLS
                     DPNLALMSIIIANVWRGTAFSMILQYAGLQSIPDELYDAAEVDGASTLQSFWYITIPQ
                     LRPILMINIILITIQTLNSFDMVLALTGGGPGQATEVLALHTYNVIFREYSLAEGAVL
                     AVIMLLISLVLTLGYRRLLRSEGTV"
     gene            complement(5351..6685)
                     /locus_tag="VN178_12210"
     CDS             complement(5351..6685)
                     /locus_tag="VN178_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS81778.1"
                     /translation="MRQWVALSVRLAVVLAVVGVVVGCGGGGGSVLTLDRIGKEDAPN
                     SMTLQLNASYTHQSSTPSWADGFERLFTKFAKDHPDWKLELEIIPDDQTTQEQARLLE
                     EARVGRAPDCANVDSFVVAQFIEQGALQPIDQYFTEEEIDGLFPFVSDIVVGDDGKMY
                     AYWFATDGRILYRRTDLVPEAPRTWDELIEAANAAEEKDPKVDGYLFNGGRWEGTTFD
                     NLAHFWSQGGELVDEQGKPIFGEEPHRTYMLNEMNFLKESVDSGASPQRVATIKDYAE
                     FEAAAQAQQVAMFQGGDFQYPTLKETLPPEEFKKWEVSLLPTRSAGDTPSSGSGGWTI
                     GSFSDDPKKVEMCMEFTKDVFIGGMNEVTGQLPTNPKYFDTINRFDEPIYDDFRVMLE
                     HGKARPGVPVYPTMSEEIQVAIGTVLTEEATPEEALDRAYKASLAAYEEQKK"
     gene            complement(6898..7839)
                     /locus_tag="VN178_12215"
     CDS             complement(6898..7839)
                     /locus_tag="VN178_12215"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWS81779.1"
                     /translation="MESAEDRAVEVVPRDYSYVFNPYREPVARVKPGDRVVIHTDDSF
                     ESRITTKDDLPSQALAAAKFLNPQTGPIYIEGAEPGDTLVVRIESIEPTREFAVSCLI
                     PYFGGLTSTIFTRTLQDPLPERVWIWNLVDGKKLVNDELGVELDWNPFMGTLAVAPDL
                     EAITALAPGPFGGNMDVPDVKPGNTIYLPVWNPGALFYTGDCHARQGQGEACGVALEI
                     TSKVTVVFDVIKGKEIEWPRIESDGAIMVVGSARPMEDAARIANVELILWLEQEHGYD
                     RWDAYQLLTQAGGLYVGNMVDTTYSLVASIEKRHLKG"
     gene            8038..9609
                     /locus_tag="VN178_12220"
     CDS             8038..9609
                     /locus_tag="VN178_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase family protein"
                     /protein_id="HWS81780.1"
                     /translation="MSEKVYRSELTPVSFLRRSAYMFPEKAAVVYGERRYSYSELEER
                     VDRLSSRLRDAGLQKGDRVAFLCPNTPPMLEGHFAVPAAGLVLVAINTRLGKDEVSYI
                     VEHSGAKMVFVDAELEKLLADVDGVETVRIDDTGEQGDPYEDYLAEGSPEPAPDVLED
                     EEETISINYTSGTTGRPKGVMYTHRGAYLSALGNAIEIGMGYETKYLWTLPMFHCNGW
                     TYPWAVTAVSGTHVCLRKVEPPRIWDLFEDEGITHYCAAPTVQIGIVNEEAAHRLETP
                     ITAAIAGAPPSPTLLGGLIELNIRPMHIYGLTETYGPMTTSGVHPEWEELEMDERARL
                     MARQGQGYATADLVRVVDENMNDVQRDGETMGEIVMHGNMVAKGYFENEEATEEAFEG
                     GWYHSGDVAVWHPDGYVEIRDRDKDIIISGGENISTIEVEQAVSRHPAVMEAAVVAIP
                     DEKWGERPKAFVVLKQGQEATEEEIIDFCKEHIARFKAPEAVEFTELPKTSTGKVQKF
                     VLRDKEWKDREKQVN"
     gene            9780..10571
                     /locus_tag="VN178_12225"
     CDS             9780..10571
                     /locus_tag="VN178_12225"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWS81781.1"
                     /translation="MIEAQKTYEHVLFEQDGAVAYVTMNRPKKRNALSLEHMRELISL
                     FQEIGEHRDASVVILRGEGPGFCAGHDLSEMIGRDPAFYRHLFDVCCELMGTIQEIPQ
                     PVIAQVHGVATAAGCQLAATCDLVVASEEARFATPGVKIGLFCSTPMVALSRAVGQKK
                     SMEMLLTGEFISAEEAFAEGLVNRVVSAEDLETETRTLAEKIAEASPLVVGIGKQAFY
                     RQLGLPTEQAYAYTKEVMSFNASFTDAQEGMCAFLEKRKPEWKGQ"
     gene            10597..10920
                     /locus_tag="VN178_12230"
     CDS             10597..10920
                     /locus_tag="VN178_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HWS81782.1"
                     /translation="MTGTIARPVEKEEQKTEGLPPYNVVLLDDNDHSYEYVILMLKRI
                     FGHPVEKGYEMAREVDATGRVVVATTNLEQAELKRDQIQAFGPDPLIPRCKGSMSATV
                     EPATA"
     gene            complement(11099..12406)
                     /locus_tag="VN178_12235"
     CDS             complement(11099..12406)
                     /locus_tag="VN178_12235"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81783.1"
                     /translation="MSPVEDTLRSTQDAYPAELARFVRERWDEDRAGTLPGQGSLETL
                     ISTCYQASLLREEERAVTFRVILCDPDLLPPREGPPDGTHRLEFPRPRRFDVQEIRRL
                     SPAADYYRSLIGVRFDEEEGLRIWGLVHTGPRWLRGGQGGRDVPPPLPPVLVLRVHGP
                     GRIAVDVGLESLCKLEEGRLSDVSMDVFDSRWLPDTFAPVRGEIAQIHTGLRDQARKK
                     GEVWARLDNDIIRVIGQHTIKRIISAVRDSHHGGTLVIVPPYLADDIVEDRYVSLKYK
                     FVDAEPRRRFRTLIVDVTNALARSYAGTPARDPVGWDDYESSSDSELTGLDEAIFEVA
                     HLIAGLTAVDGAVVMTQRFEMLGFGGEISGRLPGVKTVARALDVEADAVVKESVGDVG
                     TRHRSAYRLCNALGDVIAVVISQDGNVRFVKRKNEAVTYWDQA"
     gene            complement(12408..13574)
                     /locus_tag="VN178_12240"
     CDS             complement(12408..13574)
                     /locus_tag="VN178_12240"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWS81784.1"
                     /translation="MQGSGGSFGKTVLLAVLLVLPGAACDGAAEERGKGDNDRKRITT
                     GQGEKDTSDQSGASVERVEVSTLAADLEVPWSFAFLPGGDALVTERDSGRLLRVSPTG
                     EVQEIQTLPEGGSGEGGLLGVAVSPDYENNRYIYAYYTTRSDNRVVRFRMGERPEPIL
                     TGIPVNTYHDGGRIKFGPDGMLYVSTGDAGDSGNSQDRNSLGGKILRIEPDGSVPPDN
                     PFPGNPIYSYGHRNVEGLAWDSEGQLYASEFGASTWDEVNRIEAGENYGWPAVEGRGG
                     EDRGYVDPITVWPTTEASPSGAEIMVEGSIPQWEGDLFVAALRGERLWRLELNDSGEV
                     VDREKLLDGEVGRVRDVIQAPDGSLWVSTSNYDTYGNPVSEQDDRILRLAPAGE"
     gene            13778..15121
                     /locus_tag="VN178_12245"
     CDS             13778..15121
                     /locus_tag="VN178_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81785.1"
                     /translation="MRSYLDGLRPLLVMVFLAGYVWQTFAPDSFGTDLFLGVGTVLFV
                     ISVPWSSAFHKAFVLAALAALGTVIFTGRFDFGAFLQGMPTYFGIVAVLLVLSIAGYP
                     IQAARYEVQIRALMAALTRRGVSIKATAGGLGHVLGAVLDVGAFVLIDVISGRSAPKE
                     RLEALKWAGRTFSFAPLWTNLNVLTATTIVLTGVSYPTLLAVMLPFVLAGLGGTLFFA
                     QRAGGEVEEIPQAPLDRGAAAVLLYPVLLVSAVALVNLTFPKLPLTAAIAVTVATIVV
                     LIAVLAAVLTRRASPLARLGKETRGSLTASYSEFALFGAAGILVLSLKALGALAPLGA
                     FLSALPTVLVAPALALIMGVGFVVGIHVIPMVLLLNTAFPLDGGPAPALWAAALLLGA
                     QSTLLLTPVSSAVTMLSRLTGLHPLEIGPRRNLGFCLIMAVAALLYLGLLTLLLL"
     gene            complement(15270..16265)
                     /locus_tag="VN178_12250"
     CDS             complement(15270..16265)
                     /locus_tag="VN178_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS81786.1"
                     /translation="MTVAPSGLATQPIGEAAPVAEGVWQLKLPVPFPLRFVSVYLVEG
                     NDGWSILDAGYDYPPARAAWESGAAATGCDLGRDVDRIVVTHFHPDHIGAARWLEERT
                     RAPVYMMEREIPFARKLWGSSDAGPFVGYLIRHGMPQEAAGATAAVVRYSLPLPDEMV
                     PLRPGEKLTMGDGVARIVHVPRHADNQIVLHDETRGILFAADHLLLGITPNIGLWPES
                     EPHPLARYLESLENTRGLGADLVLPGHGPVFHDLVGRIGELIYHHEERLETMRRVVSD
                     RPMTAYEVSRIVFRGTLTDHQRCFALAETLAHLDHLVLQGQAQRIEDETITFQSA"
     gene            complement(16262..17149)
                     /locus_tag="VN178_12255"
     CDS             complement(16262..17149)
                     /locus_tag="VN178_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS81787.1"
                     /translation="MMNGLDWNHRQVDANGIRIHYVRQGGGSPLVLLHGWPEFWYTWR
                     KNIPVLAGEFDVVAPDLRGFGDTDKPGLPDPPNRLLGDFVEDLHALANSLGFERFGVV
                     SHDVGAYVAQGFARKYPERLSGLFFFDCPYPGIGRRWASPDSINEIWYQTFHQQPWAA
                     SLVGENRKTCEAYFRHFLDRWSYEPGFFDEDLDLWVDNFMKPGNLQGGFDWYTSTNES
                     RLDLIRNGPPELPKIETPTRIRWGESDSVLKVEWADRLGDYFADLDFAPMREAAHFAH
                     YERPDLANREISDFFRALS"
     gene            complement(17176..18354)
                     /locus_tag="VN178_12260"
     CDS             complement(17176..18354)
                     /locus_tag="VN178_12260"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWS81788.1"
                     /translation="MREAVIVSGARTAVGRAPKGTLRASRPDDMAAAAIGEAVRRAEG
                     LHKEDVEDVIIGCAMPEGTQGYNVARLSSLLAGMPDTVPAQTVNRFCSSGLQTIAMAS
                     ERIMVGTAKTIVAGGTEHMSSTVEMPNFSPNPRLVEINPAAYMGMGLTAEQVAHEFDV
                     SREDQDEFALRSHTLAKEAVESGVFDEEIVPLDVEFDWVDEAGEPQHVETSFGRDEGP
                     RDTSAEKLANLKPAFQQDGTVTAGNSSQRSDGAAAVVVMDREEAERRGFTPLVRFVGF
                     AVGGVRPEVMGIGPTVAIPKVLEQAGLTLGDIDLIEFNEAFAAQALAVIREVGMDLEK
                     TNVNGGAIALGHPMGATGTKLTVQLIHEMRRRGSKYGLVTMCIGGGMGAAGIFENLQN
                     "
     gene            complement(18558..20930)
                     /locus_tag="VN178_12265"
     CDS             complement(18558..20930)
                     /locus_tag="VN178_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HWS81789.1"
                     /translation="MSYRIRKVAILGAGTMGAAIAAHCANAGLEVDLLDIAPDDDDKN
                     AVVKAGFDRMQNARPAALMNDSVADRIRIGNFEEHFGRVGEADWIVEAIIEKLEPKRE
                     LMQRVEETAKEDAIISTNTSGIPLHSISEGRSEAFKRRFVGTHFFNPPRYLKLMEIIP
                     TEYTDPEIVEAVRNFGERVLGKGGVIAKDTPNFIGNRIGTFAGMQSARYAFENGYGIE
                     EVDAITGPLIGHPKTATFRLNDQVGLDIAIGVAENLYEAVPGDDSREELKPPQKLEEM
                     QEKDLLGNKTGAGFYKRGKRDGKTVFDVLDLDTFEHHPAENPEIPVAKEAQEQGDLAS
                     RLRFLIEKADEDRHARYIRDTLLPYMAYASRRVPEISDTLEDVDHAMEWGFAHQAGPF
                     RTWDLLGVRETVEKMESMDIEVASWVKEMLQAGNDSFYKTENGTELQFSPVSKEYEPV
                     REDPMYVSLDRLRGEGKELASNDSASLLDLGDGVLCLEFHSKGNCIEAGTVEMGNRAL
                     QELERDDVVGLVIGNEGRNFSVGANLGEMAHAIQNGNLDQIEESVETLQNLLMAFRFA
                     PKPVVSAPHGQTLGGGLEVCLHSDRIVAAGETYMGLVEVGVGLIPAGGGTKEMARRLV
                     SPPLHTAPDIPPLPFLQKAFEQIALAKTATSALEAKEMGFLTHNDRIVMNADHLISAA
                     KRETLDLADGYTPPEHTSNVYAAGRIARAALEMGIKTMQWGNYASAYDGVIAGHVARI
                     LTGGNLSLPQWVPEEYLLKLEKQSFLELLKQEKTHERIKALLKTGKPVRN"
     gene            complement(20906..21061)
                     /locus_tag="VN178_12270"
     CDS             complement(20906..21061)
                     /locus_tag="VN178_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81790.1"
                     /translation="MESDAASKEKYAEIKGRFEAHLLRVREAIESLHRELYGYDREAL
                     CRTGSGR"
     gene            21188..22387
                     /locus_tag="VN178_12275"
     CDS             21188..22387
                     /locus_tag="VN178_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS81791.1"
                     /translation="MIADVTRSLGTDYYLLDELFTEEEREIRDRVRAFADAEVIPIIN
                     EYWEKAEFPFELVPKVAALNIAGGPIEEYGCPGMSQVAAGLVAIEMARGDGSLNTFFG
                     VHSGLAMGTVAICGSEEQKRRWLPEMARMEKIGAWGLTEPDHGSDSVMLETTAHRDGD
                     SWVLNGEKRWIGNATFADLVIIWARDVEDEQVKAFVLEKDENGEHHPGYSTELIKGKM
                     GKRAVWQPDIHLKDVRVPDENRLEGANSFKDTNKVLTATRSGVAWEAIGHAIAAYEAA
                     LQYAQERVQFGKPIAGFQLIQNKLSGMLAEITNMQLMCFRLAQLQEQGKMTGPMASLA
                     KMNNARKAKQVCADARDIMGGNGVLLEYHVARHLSDMEIVYTYEGTDTIQSLIVGRDV
                     TGISAFV"
     gene            22600..>23102
                     /locus_tag="VN178_12280"
     CDS             22600..>23102
                     /locus_tag="VN178_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWS81792.1"
                     /translation="MKAIRLHELEGPDELVYEEVPEPEPGPGETIVRLRAAALNRRDL
                     FATQGQYPGVTPDILPIIPGSDGSGEVVARGDGAEGPDEGTGVVINPALYWGNDPRVP
                     GKEYRILGIPDEGTFAQFIKVPADHVFPKPSHLSHEEAAALPLAALTAYRALVTRGGI
                     SEGETVLV"
BASE COUNT         4131 a         7257 c         7210 g         4504 t
ORIGIN      
        1 agtacctctg gccgtagagc atctggcgca ccatagactc gccggatggg atctgacagt
       61 ccggctccac ccacgagccg cctacgggct cccagcgtcc ctcctcgacc cgcctcctta
      121 ccctctcgaa gatctcaggc gatcctgttt ctatccacct gtagagctgg gccgaggact
      181 ggttgaacac gaaatcggcg taccgctcca tgagcgagag cacgctcgcg aaggtcctac
      241 gccccttgcg catggtctcg gagacgggcc acagccaggc caggtcgagg tgcgcgtggc
      301 ccgtcaaggc gagacgccca accggcggat actcggcctt gagcttctcg agtctggagg
      361 cgaccatctc tcgcgcctcg cgcacggcac cccgcgtctc ttcggagagg ggctcgacga
      421 ctctgggggc gggcggcagg ctccacggac caccgtcggt cgactcggcg taggcctcca
      481 ggtagcgggt gagcgcgacc tcgctccccg agggccacaa agagaacagg ccggcgaatg
      541 cggtatcgac tacgtcgagg agatggggga cgacttcgtg gtcccctagc tgctcgcaga
      601 cctcgactat gagcccgagg tcacgctcga gcgcgcgaac ttccgtctct ggcactacga
      661 gcgaggaccg cctcagctgt ggctcggcga cgttggagcc gaacatcccc cttggtacga
      721 cctcggcctc gacccctacc ttctccccgc ccgcggcctt ctgtgtcacg gggaagctgc
      781 ggtggaaagg gttgaggcca ccgctgaacc ccgtcgagag actgacgaac ccttcaccgc
      841 cgagccagag ctccagttcg acgggagagc cggcccaggc ttcgggtatc ttcgcctcgg
      901 ccgagaagct cgccggcagc gcctcctcgg gccagaaatc ccccagcccg atctccttca
      961 cctccccacc atcggccgcg aagcgccact cctcgatgcg gctctcgcgc gcgttgcgcc
     1021 acagcttcag ctcctcgacg cggcgggcgt ggcgttcgag tcggtgacgg tcgcgggtgt
     1081 tcatgtatct ccttcttgtc ggcataacga cacgcccgag atactacagg ttgtccagca
     1141 tttcgcacgc gacccgactg ggtgcccatc gggggcgaag cacctggctc gtagcaggcg
     1201 acgtgcaggt tgcccgctcg ggtcactcaa agaggggtcg tacggcgtcc acgtcttcat
     1261 tctccaacac gtgccccatc ctcccgtcgg ccagagcccg ttccaccgcg cccagccaga
     1321 gggcgcgtgg ggggttgtta ccccagggtg ggcggtccat gccctcgtaa cggtcgaaca
     1381 ctaccggagg ctgcatctgc tgccgtgcca aggtgcgcag catggtctct tccaggcgcc
     1441 cgcccacaca gaggacgaag gcgaaggttt gcgggccctg gtccgtgtac cggtcacgcc
     1501 cggcatacac tacaggttct ccgcctcccc aggccatctt cgggctcctg agcagggtcc
     1561 actgccagca gtcgccgtcc aggctgaggc tataggcatc gcctgtcaca agggccaggt
     1621 gccggtccgc aagctccacg tgggaccagc cctgcaccgg ccattccgtc gcgctggcgg
     1681 tgcgctctac atggccaccc ggcaacccat cggtcctgcg ggtggggaga gccgcgagat
     1741 gtacgggcat ctgcagcagc ttgaaacgct cgctgaagtt gacctctact cgtatgtgaa
     1801 tccggggctc gtagcggtgc agcgtgaggg tccatcgcac ggaggagcga cccagccagc
     1861 cctccgagcg tactcgcgcc cgcaatggcc cggtctcctc cacgacccat ccgtcggtat
     1921 gtagaaccgt ctctatgggc tcgacgaaac ggtctgtgtg gaaggtccag gtgtcggtga
     1981 gatccctgcg caggtgcagg ccgaagccac cctctcccag caggtctctt cctcctcctg
     2041 cgatcaggcg ccggatcccg gagccgttca actccgcgcg caggtggccg ttcgcgatga
     2101 gatgcggcga ggcctccaga tccgagtctg gtgcttcggt ggaagggcgt ttatctgttc
     2161 tcaccagaac ctgggcgtac tgccctccgg ctagagtgag tgggaagacc acgcgctcca
     2221 cgtgggctgc cgctgctggc tgcacgacct gaaagtctat gggcctacct tcgaggtcac
     2281 tcaaccaccg gttgccccat gcgtcgaagc tgaggaacgg ctccgtctcg accgtgcctt
     2341 cccagggggc gtcgtcgggg ttgataatca ctatctgctg ctcgtcgagc ggcggcaatt
     2401 tacgatgcgc ccagcgccgg gtggcctcca cggcgacctc ctcgccggtt atcctggccc
     2461 ggccctgcat cgccctgatc gaatcccagg ctgccggaac cgaggtgcct gcgagaacgt
     2521 catgaaactc cgtgaacaac aggtcgtccc aggcaccgtc gagccgttcg tgtaaccccc
     2581 gtctctcctc ttcatcgtcc acgaaaccct ccacgagacg cccgcactga tctaggaggt
     2641 gctcgccacg ctgctgttgc tgcttgatgt cgtgcatcac gctgtaacag cccgggaatg
     2701 tgtgttgtag ctcgtcggtt acgattggga gacgatctct ggactgttcg atagcgtcga
     2761 agaaggcctg gggtgtgctg aatcgtagtt ctgtgtcagg aaacgatcgt gtgttctcga
     2821 ggatgtattc gatgttggcc ttggtcggtc caccgccgtg gttgcccacg ccgtagaagc
     2881 acatggtgtg tccgagttcg gggtttgccg cctcgatgga gagcatgatc tggccgtaca
     2941 gatcgtccga gaacgtgacg taggcaggag agattcgaaa gccgatcacc tcctccccgc
     3001 cgacaccacg ccaccgaaag gtctgggccg gcaactcgac ctggtgggct ccgggtcgat
     3061 ggaagacgta gcctacgtag ccatgggcag cgagcacgtc gggcagggtg gcggggtggc
     3121 cgaaggaatc cacgttgtag cccaccttcg ggctaacacc gaaacggtcc tcgaagtagc
     3181 gctggccgtg gatgatctga cggtgcaggc ccatgaccgt ggagaggttg acgtcgggct
     3241 ggatgtactg gcctcccgtg atgtgccacc gaccgcttgc gaccagcctc cgcaccctct
     3301 cgaagagccc agggtcgatc cgctccacct gctcgaagag ccaggattcc ccgcgggtga
     3361 atacgaactc ggggtactca tcgcagcggt ccgcggcgga acggaacgtg gccagcgcct
     3421 cgtccacccc ggcctgccag ggccacagcc acacagggtc gatgtgggcg tttcctatca
     3481 tgtgtactat cacgaaagga ctaatcccct cggtttgcct acgcttgcct ttcctaccta
     3541 ccgacgccgg acagagtcgg cgcgtacgga cgtgctcccc agactagccc ttgatggctc
     3601 cgcccatgag ggcgcggacg atgaaccgct gcaggatgac gaaggtggcg atggccggca
     3661 gcatggccag cacacctccc gcggcgacca cttgttgcga ggtctgggcg tagttgccct
     3721 ggaagttgag gattccgatc gagatcggga gcagcgaggg cttgctgagg agtatgtagg
     3781 gtatggtgaa ctctccccag gcgaggatcg ccgtcagcac gaacgccgag gtgaggcctg
     3841 gtaggatgag cggcagggta accctccaga acgcgccgaa gcgcgtgcaa ccgtctacca
     3901 tcgccgcctc gtccagagcg ggagggatgc cgtcgaagaa acctttgagc atccaggtgg
     3961 cgagcggcac caggatggcg atgtatacca tcgccgtgct gacgtgcgag tcgagtatgt
     4021 tcaggcgggc gaagtaccta tagagcggga cgccgatcac cagcggagat atcatctgca
     4081 gggcgaggac cccgatcagc agcgtgctgt tgccgcggaa ggtaaggcgg gagaaggcgt
     4141 acgccgccgg tgcggcgacc accatcgcgc cgacgccccc cgccacggag agcttgagcg
     4201 agttgatcag gaacacagag aactgcgcag agtacagcac ctcgacgtag ttctcgagcg
     4261 tcggggactc cgggatcagc ctgagcgacg taatgtagac ctcctgcggg gtccggatcg
     4321 agagggagag cacccacaag agcggtccgc cgaagaacac cagcgcggcg gcgaacacca
     4381 ggtaggtcag cgcgtcgccg atcctgcccc tggccttgcg gcttctcata ctgttccctc
     4441 cgatcgcagc aatctcctgt aaccgagggt gaggaccagg ctgatcagga gcatgatcac
     4501 ggcaagcacg gcaccttcgg caaggctgta ctccctgaag atgacgttgt acgtgtggag
     4561 ggcgagcacc tccgtcgcct gccctgggcc accacccgtg agcgctaata ccatgtcgaa
     4621 gctgttgagg gtttgaatcg ttatcaggat gatattgatc atgagtatcg gcctgagctg
     4681 cggtatggtg atgtaccaga aggactgcaa cgtcgatgcg ccgtccacct ctgcggcatc
     4741 gtagagttcg tctgggatcg actgcaggcc ggcgtattga agaatcatgc tgaaggcggt
     4801 cccccgccag acgttggcga ttatgatcga catcagggcg aggttggggt cggaaagcca
     4861 cgccttgccg cccagcccga cgggggcgag caccagattg gccagcccgt agctcgcctc
     4921 gtttagaatg atggcccaca cgatcccgat gaccacgccg ggcatgaccc aggcggagag
     4981 gattatcgag cgcatgaaga ccatgccggg aagacgccgc ctcctggccc tgttgacggc
     5041 taccgcgatc agcagcccga gagtgagctg caagacgacg ctcgccgcga cgaagatggc
     5101 cgttatccga agcaccttgt acaggacagg atcgctcagg acgttcttga acgtgtttag
     5161 cgtatagtcg taattcggct ccagcaatga ggcgtcggtg atgctgaaac gcagaacctc
     5221 gaacgccggg tacaggtaga acagcgtgac gaccgcgagc aacggggcca tccagagcgt
     5281 gggcctgttc tcgcctatga gctgccgagg agcgaaggcc tttttcgtca tgacttttcc
     5341 taatccaccg ctacttcttc tgctcctcgt aagccgcgag tgaagccttg tacgccctgt
     5401 cgagggcctc ctccggcgtc gcctcctcgg tgagtaccgt gccgatggcg acctggatct
     5461 cctcggacat cgttgggtag accgggaccc caggccgggc cttcccatgc tcgagcatga
     5521 cgcggaagtc gtcgtagatc ggctcgtcga acctgttaat ggtgtcgaag tacttcgggt
     5581 tggtcggcaa ctggcccgtg acctcgttca tgcccccaat gaagacgtcc ttggtgaact
     5641 ccatgcacat ctcgaccttc ttggggtcgt cggagaagga gcctatggtc catccgccgc
     5701 ttcccgacga cggcgtgtcc ccggcgctcc ttgtcggaag tagggagacc tcccacttct
     5761 tgaactcttc gggcggcagg gtttccttca aggttggata ctggaagtcg ccgccctgga
     5821 acatcgcgac ctgctgtgcc tgggccgccg cttcgaactc ggcgtagtct ttgatcgtcg
     5881 cgacccgttg gggtgaggcg ccgctgtcca cggactcttt gaggaagttc atctcgttca
     5941 gcatgtacgt gcggtgcggc tcctcgccga agatcggctt gccctgctcg tcgaccagct
     6001 cgccaccctg gctccagaag tgggccaggt tgtcgaaggt ggttccttcc catctcccgc
     6061 cattgaagag gtagccgtcg accttgggat ccttctcttc ggccgcgttc gccgcctcta
     6121 tgagctcgtc ccacgtcctc ggcgcctccg gcaccaggtc cgtccttcgg tacaggatcc
     6181 gcccgtccgt ggcgaaccag taagcgtaca tcttcccgtc atcaccgacg acgatgtcgc
     6241 tgacgaaggg gaacaggccg tctatctctt cttcagtgaa gtactggtca atcggctgca
     6301 aagccccctg ctcgataaac tgtgcgacga cgaaggagtc tacgttggcg cagtcaggcg
     6361 cccgcccgac cctggcctcc tcgagcaggc gcgcctgttc ctgggttgtc tgatcgtccg
     6421 ggatgatctc gagctcgagc ttccagtcag gatgatcctt agcgaacttc gtgaagagcc
     6481 tctcgaagcc gtcagcccaa ctcggtgtcg aggactggtg ggtgtagctg gcgttgagtt
     6541 gcagcgtcat cgaattcggt gcgtcctctt tacctatgcg atccagcgtc aaaacgctgc
     6601 cgccaccacc tccacaaccg acgacgaccc cgaccacggc cagaaccaca gccagccgaa
     6661 ccgatagcgc gacccactgt ctcatatagc acccaccccc tagatcgcac ggcgcctgac
     6721 acgcacggcg atctccgtcg acgccccttc accagtcagc gttccccacg ccagcactat
     6781 aacagtaacc tagcagccgc tgcgcatcaa gtgctacaag ggatcgtaac tgtcccacag
     6841 acggtgggtg tgggacggtt accacgaagc tcgggtgtct ttctgggccg gctcacatta
     6901 acccttcaga tgcctctttt cgatagatgc caccaaagag taggtagtgt cgaccatatt
     6961 gcccacgtac aggccgcccg cctgggtgag cagttggtag gcgtcccagc gatcgtaccc
     7021 gtgctcctgc tccagccaca agatgagctc tacgtttgcg atgcgggccg cgtcctccat
     7081 cgggcgggcg ctgccgacga ccatgatggc gccgtccgac tcaatgcggg gccactcgat
     7141 ctctttgccc ttgatgacgt cgaacaccac cgtcaccttg gaggtgatct ccagcgcgac
     7201 gccgcaggct tcaccctgac cctgccgggc gtggcagtcg ccggtgtaga agagtgctcc
     7261 ggggttccat acgggtaagt agatggtgtt tcccggcttc acatccggca cgtccatgtt
     7321 cccgccgaat ggacccggtg ccagagcagt gatggcctct aggtcgggag ccacggcgag
     7381 tgttcccatg aaagggttcc agtccagttc cacgcccagt tcatcgttga ctagcttctt
     7441 gccgtctaca aggttccaga tccagaccct ctcagggagc gggtcctgga gcgtccgggt
     7501 gaagatggta gacgtgaggc cgccgaagta gggtatgagg caactcacgg cgaactctcg
     7561 cgtcggctcg atgctctcga tcctaacgac aagggtgtcg ccgggctcgg ctccttctat
     7621 gtagatcggt ccagtttgag ggttgagaaa tttggcggcg gccagggcct ggctggggag
     7681 atcatccttc gtggttatcc ggctttcgaa agaatcgtct gtgtggatca ccacgcgatc
     7741 ccccggtttc accctagcca cgggttcacg gtaggggttg aagacgtagc tgtagtctcg
     7801 ggggactacc tccaccgcgc gatcctcggc gctctccata tgcggccctc cttgatattg
     7861 ccgatccgcc cctaatggtc ctcatgtttg gcactggcgc ggacgggttc atagtaccgc
     7921 gccttagctg cggcggtaat ggcaacgtgg agcagtggac cggtctgctt cggcctagtc
     7981 tcataccggg aagtgtgttg taatccgcca cgggaatagc aacaatggag gagcagcatg
     8041 agcgagaagg tctaccgcag cgagcttaca ccggtcagct tcctcaggcg tagcgcctac
     8101 atgtttcccg agaaggccgc cgtcgtctac ggcgagaggc gttactccta tagcgagctc
     8161 gaggagcggg tcgaccgcct gtcctcgcgg ttgcgggacg ccgggctgca gaagggtgac
     8221 cgcgtcgcct tcctctgccc taacacgccg cccatgctgg agggccactt cgccgttccc
     8281 gcagcggggc tcgtgctcgt cgccataaac acccgtctcg gcaaggacga ggtctcctac
     8341 atcgtcgagc attcgggggc gaagatggtc ttcgttgacg ccgaactgga gaagctgctc
     8401 gccgacgtcg atggcgtcga gaccgtgcgc atcgacgata caggagagca gggagacccg
     8461 tatgaggact acctggccga aggctcgccc gagcctgcgc ccgacgtgtt agaagacgaa
     8521 gaagagacca tctctatcaa ctacacgtcg gggacgacgg ggcgtcccaa gggcgtgatg
     8581 tacacgcacc ggggggcgta cttgagcgcg ctcggaaacg cgatcgagat cgggatgggc
     8641 tacgagacca aatacctgtg gacccttcct atgttccact gcaacgggtg gacgtacccg
     8701 tgggcggtca ccgccgtttc cgggacgcat gtatgtctca ggaaagtcga gccgccgcgc
     8761 atctgggacc ttttcgagga tgagggcatt acgcactact gcgccgcccc gaccgtccag
     8821 atcgggatag tcaacgagga agcggcgcac cggctggaga cgcccataac cgccgccatc
     8881 gcgggcgctc cgccttcccc caccctgctg ggcggcctca tcgaactgaa catccgtccc
     8941 atgcacatct acggcctcac cgagacctac ggcccgatga ccacgagcgg ggtacatccc
     9001 gagtgggaag agctggagat ggacgagcgg gcgaggctca tggcccgtca gggtcaggga
     9061 tacgcgaccg ccgacctcgt gcgcgtggtt gacgagaaca tgaacgacgt gcagcgcgac
     9121 ggggagacca tgggtgagat cgtgatgcac ggcaacatgg tcgccaaggg ctacttcgag
     9181 aacgaggagg cgaccgagga ggcgttcgag ggtggctggt accactccgg cgatgtcgca
     9241 gtgtggcacc ctgacggata tgtagagatc agggaccggg acaaggacat catcatctcc
     9301 ggcggcgaga acatctcgac catcgaggtg gagcaggccg tgtcgcgcca cccggccgtc
     9361 atggaggccg ccgtcgtcgc catcccggac gagaagtggg gcgagcgccc caaggcgttc
     9421 gtcgtgctca aacaggggca ggaggcgacc gaagaggaga tcatagactt ctgcaaagag
     9481 cacatcgccc gtttcaaggc ccccgaggcc gtcgagttca ccgagctccc caagacctcg
     9541 acgggcaaag tccagaagtt cgtcctgcgc gataaggagt ggaaagatcg cgaaaagcag
     9601 gtgaattaga ggtgtcaggc ttaaggttac aggcttcagg gcttggagtc gaaactctga
     9661 ggcggagaat gggccggttt catctggtga gtggcaacac acgccggggt ccgtccgaca
     9721 tacgactcga aacctgatgc ctgcagcctg atacctgaag cctatacgaa ggaggcgtta
     9781 tgatcgaagc ccagaagacc tacgagcatg tcctcttcga gcaggacggc gctgttgcgt
     9841 acgtgaccat gaaccggccg aagaagagga acgccctctc actcgagcac atgcgggagt
     9901 tgatctctct attccaggag ataggagagc acagggacgc ttcggtggtc atcttgcgtg
     9961 gcgagggtcc cggcttctgc gcgggccacg acctctcgga gatgatcgga agggatccag
    10021 ccttctaccg ccacctcttc gacgtctgct gcgaactcat ggggacgatc caggagatcc
    10081 cacagcccgt gatcgcgcag gtccacggcg tcgccaccgc cgcgggctgc cagctcgccg
    10141 ccacctgcga cctcgtggtg gcctccgagg aggcacggtt cgccacaccc ggagtcaaga
    10201 taggactgtt ctgctccacc ccgatggtcg cactctcacg ggccgtcggc cagaaaaaat
    10261 ccatggagat gctcctcacg ggcgagttta tctctgccga agaagccttc gcggaaggac
    10321 tcgtcaaccg ggtggtttct gccgaggacc tcgagaccga gacacgtact cttgccgaaa
    10381 agatcgcgga ggccagccct ctggtcgtgg ggatcggaaa gcaggcgttc tatcgtcagc
    10441 taggattgcc cacggagcag gcatacgcct acaccaaaga ggtcatgtct ttcaacgcct
    10501 cgttcaccga cgcccaggaa gggatgtgcg cctttttgga gaagcgcaag cccgagtgga
    10561 agggacagtg ataaaggctt caggatgtga actcggatga caggcacgat cgcgcgtccg
    10621 gtagagaagg aagagcagaa gaccgaggga ctgccgcctt acaacgtggt tttgctcgat
    10681 gataacgatc acagctacga gtatgtgatc ctgatgctga agcggatctt cgggcatccc
    10741 gtggagaagg ggtatgagat ggcgcgggag gtcgacgcca cagggcgggt cgtcgttgcg
    10801 accaccaacc tggagcaggc cgaactcaag cgggatcaga tccaggcctt cggccccgac
    10861 ccgctgatcc cgcgctgcaa aggctccatg tccgcgactg tagagcccgc gacggcctga
    10921 cggccgccgc ttgtcagcac ggcggctttg ccgccttgtc agcacttcag cacgccgccc
    10981 ggcttcgccg gacggcttgt cagccagggc tcttgcgctc gcaaaaactg acacgctgaa
    11041 agcgagggcc tgaaaggtcc gaagcgtgct gacaagcctg cgaagcaggc gtgctgactt
    11101 acgcctggtc ccagtaggtt acggcttcgt tcttgcgttt gacgaagcgc acgttgccgt
    11161 cctgggagat gacgacggct atgacgtcgc cgagcgcgtt gcagagccgg tatgcggagc
    11221 ggtgtcgggt ccccacgtcc cctaccgact ctttgacgac ggcgtctgcc tcgacatcca
    11281 gggctctggc gaccgtcttt acgccgggta gccgaccgga gatctcacca ccgaaaccga
    11341 gcatttcgaa gcgctgggtc ataacgacgg cgccgtccac ggcggtgagg ccggcgatca
    11401 ggtgcgccac ctcgaagatg gcctcgtcga gcccggtcag ttcgctgtcg ctgctcgact
    11461 cgtagtcgtc ccagccgacg gggtcccttg ccggtgtgcc ggcataggag cgggccaggg
    11521 cgttcgtcac atcgactatg agggtgcgga agcggcggcg cggctccgcg tcgacaaact
    11581 tgtacttgag ggatacgtag cggtcttcga ctatgtcgtc ggccaaatac ggcgggacga
    11641 tgacgagggt tcctccgtgg tgggagtccc taacggcgga gatgatacgt tttattgtgt
    11701 gttggccgat cacgcggatg atgtcgttgt caaggcgggc ccacacctcg cccttcttcc
    11761 gtgcctggtc gcggagtccg gtgtggatct gcgcgatctc gccccttaca ggggcgaatg
    11821 tgtcggggag ccagcgcgag tcgaatacgt ccatcgagac atccgagagt ctgccttctt
    11881 ccagcttgca aagggattcc agcccgacgt cgacggcgat gcgtcccggg ccgtgtaccc
    11941 tgaggaccag tacaggcggc aggggaggcg ggacatccct acccccctgt cctcccctca
    12001 accatcttgg tcccgtgtgg acgaggcccc agatcctcaa cccctcttcc tcgtcgaaac
    12061 ggaccccgat gagcgagcgg tagtagtcgg ctgcgggtga gagccgcctt atctcctgga
    12121 cgtcgaaccg cctgggcctc ggaaactcga gcctgtgcgt cccgtcgggt ggtccttcac
    12181 gcggcggcag caggtcggga tcgcagagga tcaccctgaa cgtaacggcg cgctcctcct
    12241 cacgcaacag actggcctgg tagcaggtcg agatcagggt ctccagcgaa ccctggcctg
    12301 gcagcgtacc ggctctatcc tcatcccacc gctcgcggac gaagcgggcg agctctgccg
    12361 ggtatgcatc ctgggtactt ctcagcgtgt cttccacggg gctcattcta ttctccggcc
    12421 ggggcgaggc gtagtatgcg gtcgtcctgc tcgctgaccg ggttaccgta ggtgtcgtag
    12481 ttggatgtcg agacccacag ggacccgtct ggggcctgta tgacgtcccg cacgcggccg
    12541 acttccccgt cgagaagctt ctccctatcc acgacctcgc cgctatcgtt caactccagg
    12601 cgccagagcc gctctccgcg gagggctgcc acgaagaggt cgccctccca ctgcgggatg
    12661 gacccttcga ccatgatctc ggcaccgctc ggggaggctt ctgtggtcgg ccagacggtg
    12721 atagggtcga cgtagccccg gtcctctccg cctctgccct cgaccgctgg ccagccatag
    12781 ttctcgccgg cttcgatcct gttgacctca tcccaggtgc tcgcgccgaa ctcactagcg
    12841 tagagttgcc cctccgagtc ccacgctagc ccctctacgt tgcggtggcc gtaagaatag
    12901 atggggttgc cagggaacgg gttgtccggt ggaacggaac catctggctc tatcctaagg
    12961 atcttgcctc ccagagagtt ccggtcctgg gagttcccgg aatctcctgc gtcgcccgtg
    13021 ctcacgtaga gcatgccgtc ggggccgaac ttgatcctgc ccccatcgtg ataggtgttt
    13081 acgggtatcc ccgtcagtat gggctccggc ctctcgccca tgcggaacct tacgacccgg
    13141 ttgtctgacc tggtcgtgta gtaggcgtag atatagcggt tattctcgta gtcgggggag
    13201 acggcgacgc cgagaagacc gccctcgccc gagccaccct ccggaagggt ctggatctcc
    13261 tgaacctcac ctgtggggct cacccgcaga agcctccccg agtcgcgctc ggtaacgagg
    13321 gcgtcgccac ctggtagaaa cgcgaacgac cagggtacct ccaggtccgc ggcgagcgtg
    13381 ctcacctcga cccgctccac agaagcccca ctttgatccg aggtatcctt ctctccttgc
    13441 cccgtggtga tccgctttct gtcgttgtca ccctttcctc gctcttcagc ggccccgtcg
    13501 cacgccgcgc ccgggagaac gagcaggacg gcaagtagca cagtcttgcc aaacgagccg
    13561 ccagatcctt gcatgttcac gtcctctcta ctacgtagac ggagattata ttgcgcacag
    13621 taccgcgacc gccttgcagc cggcggtcga ttggtcccgc aaagccccct actggattca
    13681 tccatgctct agaatcgtgg accaggactg tattttgtct gctcggaagc gggtcagagg
    13741 tgatcgagaa caccgaaaaa cagcaggggc agggagcgtg aggtcgtatc tggacgggtt
    13801 gcgtcccttg ctcgtcatgg tctttttggc cggatacgta tggcagacgt tcgcgccaga
    13861 ctctttcggg accgacctct ttctgggggt gggaacggtg ttgttcgtga tctccgtgcc
    13921 gtggtcgagc gccttccaca aggctttcgt cctggccgcc ttggccgctc taggcacggt
    13981 gatctttact ggacggttcg acttcggagc ctttctacag ggcatgccta catacttcgg
    14041 gatagtggcc gtgttgctcg tcctcagcat cgccggctac ccgatccagg ccgcccgtta
    14101 cgaggtccag atcagggccc tgatggccgc cctaacccgc cggggagtaa gcatcaaggc
    14161 gacggccggg ggcctcgggc atgtcctcgg tgctgttctc gatgtgggag cattcgtgct
    14221 catagacgta atctcgggcc gctccgcacc taaagagcgc ctggaggcct tgaagtgggc
    14281 cgggcgcacc ttctccttcg cacccttgtg gaccaacctg aacgttctga cggcgacgac
    14341 catcgtgctg accggggtca gctacccgac gctcctcgcg gtcatgctgc ccttcgtgct
    14401 cgccggcctc ggcgggacgt tgttcttcgc ccagagagca ggaggcgaag tggaagagat
    14461 ccctcaggca cccctggacc ggggcgctgc cgcagtcctc ctctatcccg tcttgctcgt
    14521 ctctgcagtc gcgctggtca acttgacctt tcccaagctc ccgcttacgg cggccatcgc
    14581 ggtgacggtc gcgaccatcg tagtgctaat cgcggtactc gcggctgtgc tcacccgcag
    14641 agcgtcgccc ctggcgcggc tcgggaaaga gacacgaggc tcgcttacgg cctcgtactc
    14701 cgagttcgcc cttttcggag ccgccggcat cctcgtgctc tccctgaaag ccctcggcgc
    14761 tctagcaccg ctaggcgcat tcctctctgc cttgcccacg gtcctcgtcg cacccgcgct
    14821 cgcgctcatt atgggcgtgg gtttcgtcgt gggcattcac gtcataccga tggtgctgtt
    14881 gctcaacaca gcgtttccgc tcgacggcgg accggcacct gccctgtggg cggctgccct
    14941 cttgctgggc gcccagtcca cactcttgct cacacctgtt tccagcgccg tcaccatgct
    15001 ctcgcggctt accggactgc acccgctgga gataggaccc aggcgaaacc tgggattctg
    15061 cctcatcatg gccgtcgccg ctttgctgta tctgggcctt cttacgctgt tgctcctgta
    15121 atggcagctt tgggaagggc cgatctttcc caaagccgcg ccggtcagca tttcagcccg
    15181 ccgcccgcag ggcggcttgt tggcacgtca cttcgcacct tgtcagccag tcggctaacg
    15241 agaagcttgc ctgttgaagt gcgtaggaac tatgcggact ggaaggtgat ggtctcgtcc
    15301 tctatccgct gtgcttggcc ctggagaacg aggtggtcca ggtgcgccag ggtctcggcg
    15361 agcgcgaaac accgctggtg atctgtcagg gtcccgcgga acacgattcg tgagacctcg
    15421 tacgcggtca tcgggcggtc ggagactacc cgccgcatcg tctccagccg ctcctcgtgg
    15481 tggtagatca actcccctat ccggccgacc aggtcgtgga agacgggtcc gtgcccgggg
    15541 agtacgaggt ctgcgccgag gccgcgcgtg ttctccagag attccaggta gcgggctagc
    15601 gggtgcggct cggactcggg ccagagtccg atgttcgggg tgataccgag cagtagatgg
    15661 tccgcggcga agaggatgcc gcgcgtctcg tcgtgcagca cgatctgatt gtccgcgtgc
    15721 cgaggaacgt ggacgatgcg ggccacgcca tcgcccattg tcagcttctc gccgggtcga
    15781 agcgggacca tctcgtccgg tagcggcagt gagtagcgga ccacggcggc ggtagctcct
    15841 gctgcctcct gtggcatccc gtgccggatc aggtacccga cgaaaggccc ggcatccgag
    15901 gaaccccaga gtttacgggc gaaggggatc tcccgctcca tcatatagac cggcgcccgg
    15961 gttcgttcct ccagccagcg tgccgccccg atgtggtcgg ggtggaagtg cgtcaccacg
    16021 atccggtcta cgtcccgccc gagatcgcag cctgtagcgg ctgccccact ctcccacgct
    16081 gcgcgggccg gcgggtaatc atagccggcg tccaggatgg accagccatc attgccctcg
    16141 acgaggtaca cggagacgaa gcgcagcggg aacggcacag gcagcttgag ctgccagacg
    16201 ccctccgcca ccggggctgc ttcgccgatg ggttgcgtgg cgagcccgga gggggcgacg
    16261 gtcacgatag cgccctgaag aagtcggaga tctcacggtt ggcgagatca ggccgctcgt
    16321 agtgggcgaa atgggcggct tctctcatgg gggcgaagtc gaggtcggcg aaatagtctc
    16381 cgagccggtc ggcccactcc accttcagca cgctgtcgct ctcaccccac ctgatgcggg
    16441 ttggcgtctc tatcttcggc aactcggggg ggccgttgcg gatgagatcc aacctcgact
    16501 cgttggtgct cgtataccag tcgaacccac cctgcagatt gccaggcttc atgaaattgt
    16561 ccacccagag gtcgaggtcc tcgtcgaaga aacccggttc gtaagaccag cgatctagga
    16621 agtggcggaa gtatgcctcg caggttttcc ggttctctcc caccagggaa gcggcccacg
    16681 gctgctggtg gaatgtctgg taccagatct cattgatgct atctggcgac gcccatcgcc
    16741 tccctatccc cggataggga cagtcgaaga agaacagccc gctgagacgt tctggatact
    16801 tcctcgcaaa cccctgcgcc acgtaggcac ccacatcatg gctgaccact ccgaacctct
    16861 cgaagcccag agagttcgcg agggcgtgca gatcctccac aaagtcgccg agtaacctgt
    16921 tgggaggatc gggcaggccc ggcttgtccg tgtccccaaa gccccgaaga tcgggagcga
    16981 ccacgtcgaa ctctccggcg aggaccggta tgttcttgcg ccacgtatac cagaactcgg
    17041 gccaaccgtg cagcagcacc agcggggaac cgcccccttg gcggacgtag tgaatcctga
    17101 tgccgttggc gtcgacctgc cggtggttcc agtcgagtcc gttcatcatg cctgtcctat
    17161 gctgaatgct gaccgctaat tctgcaggtt ctcgaagata cccgcggcac ccatacctcc
    17221 accgatgcac atggtgacga gaccatactt cgaaccgcgc cgcctcatct catggattag
    17281 ctggaccgtg agcttggtgc ccgtcgcacc catggggtgg cctagcgcga tggccccgcc
    17341 gttgacgttg gtcttctcca ggtccattcc gacctcccgg atcaccgcca gagcctgggc
    17401 cgcgaacgcc tcgttgaact cgatgaggtc gatatcgccc aaggtgagcc cggcctgctc
    17461 caacaccttg ggaatggcca cggtcggccc tatgcccatg acctcgggcc tgacgccccc
    17521 cacggcgaaa ccgacgaagc ggacgagcgg cgtgaacccg cgcctctcag cctcctccct
    17581 gtccatcacc acgaccgccg ccgccccgtc gctcctttgc gacgagttac cagccgtgac
    17641 ggtgccgtcc tgctggaagg caggcttgag gttggcgagc ttttcggccg aggtatctcg
    17701 cgggccctcg tcgcgcccga agctcgtctc cacgtgttgt ggttcaccgg cctcatctac
    17761 ccagtcgaac tccacgtcga ggggtacgat ctcctcgtcg aaaacgccgc tttcaaccgc
    17821 ctctttcgcc aacgtatggc tgcgcagggc gaactcgtcc tggtcttcgc ggctaacgtc
    17881 gaactcgtgg gctacctgct ccgcggtcag gcccatgccc atgtaggcgg cggggttgat
    17941 ctccacgagc cgcggattgg gcgagaagtt gggcatctcg acggtcgaag acatgtgctc
    18001 tgtccctccc gccacgatgg tcttcgcggt gccgaccatg atccgttcgg aagccatcgc
    18061 gatggtctgt agccccgagg aacagaaccg gttgaccgtc tgtgccggca ccgtgtcggg
    18121 catcccggcg agcagcgaag acaacctggc gacgttatac ccctgcgtcc cttcaggcat
    18181 cgcacacccg atgatcacgt cctctacatc ctccttgtga agcccttccg cacgcctaac
    18241 cgcctcaccg atagcggcgg cggccatgtc gtcggggcgg ctggcccgca aagtcccctt
    18301 cggcgcacgc cccacagcgg ttctggctcc tgaaacgatt accgcttccc tcattgtcgc
    18361 tcctttcagt cgctcagctt ttcagcacgt cgcctgttcc aggcgacttg tcagcatatc
    18421 agcacgcctg cttcgcaggc ttgtcagcat ttcagcttcc gcttgcggaa gtcctggccg
    18481 acgatgcggc gcagcgccgt gctgaagtgc tgacaagcga aggccgcaag gcctgagtgt
    18541 gctgactagc tgaaatgcta attccttacc ggctttcctg ttttcagcaa tgccttgatg
    18601 cgttcgtggg ttttctcttg tttcagaagt tcgaggaatg actgcttctc caacttcagg
    18661 aggtactctt cggggaccca ttgggggagc gagaggttgc cgccggtgag gattcgggcg
    18721 acgtggcctg cgatgacgcc gtcgtaggca ctcgcgtaat tgccccattg catggtcttt
    18781 atgcccatct ccagggctgc acgtgcgatg cgaccggcgg cgtagacgtt gctggtgtgc
    18841 tcgggcggcg tatagccgtc tgccaggtcc agggtctcgc gtttggctgc ggagattagg
    18901 tggtctgcgt tcattactat gcggtcgtta tgggttagga agcccatctc tttcgcttcc
    18961 agggcgctgg tcgcggtctt ggccagcgct atctgctcga aggctttctg gaggaagggc
    19021 agtggtggta tatccggggc ggtgtgtagc ggcggggaga cgaggcggcg ggccatctct
    19081 ttggtgcctc ctccggcagg gatcaggccc accccgacct cgacgaggcc catgtaggtc
    19141 tcgcctgcgg ccacgatgcg gtccgagtgg aggcacactt cgaggcctcc gcccagggtc
    19201 tggccgtggg gggcggagac tacaggcttc ggggcgaagc ggaaggccat caggaggttc
    19261 tgcaaagtct cgacgctctc ttcgatctga tcgaggttcc cgttctggat ggcgtgggcc
    19321 atctctccga ggttggcgcc tacggagaag ttgcggccct cgttgccgat gacgagcccg
    19381 accacgtcat cacgctccag ctcctgcaga gcgcgattgc ccatctcgac agtaccagct
    19441 tcgatgcagt tgcccttgga gtggaactcc aggcatagga ccccgtcgcc gagatcgagc
    19501 aggcttgccg agtcgttgct ggcgagctct ttgccctcgc cccgaagtcg gtcgagcgag
    19561 acgtacatgg ggtcttcgcg gacgggttcg tactccttgc tgaccgggct gaactggagt
    19621 tcggtgccgt tctcggtctt gtagaaagag tcgttgcccg cctggagcat ctcctttacc
    19681 caggaggcga cctcgatgtc catcgactcc atcttctcca cggtctcgcg tacgccgagc
    19741 aggtcccacg tcctgaatgg tcctgcctgg tgggcgaagc cccactccat ggcgtggtca
    19801 acgtcctcca gggtgtcgga gatctcaggt acgcgacgcg aggcgtacgc catgtacggc
    19861 aacaaggtgt cccttatgta gcgggcgtgc ctgtcctcgt cggctttctc gatcaggaac
    19921 cgcagcctgg aggcgagatc tccctgttcc tgcgcctctt tggcaacggg gatctcgggg
    19981 ttctccgcgg gatggtgctc gaaggtatcg aggtccaaca cgtcgaagac ggtcttgccg
    20041 tcgcgtttgc ctcgtttgta gaaaccggcg ccggtcttgt ttccgagcag gtctttctcc
    20101 tgcatctctt cgagcttctg cggcggcttg agctcctcgc gcgaatcatc cccggggacg
    20161 gcctcgtaga ggttctcggc cacgccgatg gcgatgtcca gtccgacctg gtcgttgagc
    20221 ctgaaggtcg ccgtcttcgg gtggccgatg agaggtcctg ttatggcgtc gacctcttcg
    20281 atcccgtagc cgttctcgaa ggcgtagcgc gcggattgca tccctgcgaa ggttccgatc
    20341 cggttaccta taaagttcgg ggtgtccttg gcgatgacgc cgcccttgcc gagcacccgc
    20401 tctccgaaat tacggacggc ctcgacgatc tctgggtccg tatattctgt gggtataatc
    20461 tccatgagct tcaagtatcg cggcgggttg aagaagtggg tgcccacgaa acgcctcttg
    20521 aatgcttcgg accgcccttc ggagatgctg tgcaacggta tgcccgaggt attggtcgag
    20581 atgatcgcat cttctttcgc cgtctcttcg acgcgctgca tgagctctct cttgggttcg
    20641 agcttctcga ttatggcctc gacgatccaa tccgcctcgc cgacgcgccc gaagtgctct
    20701 tcgaagttcc cgattcttat cctgtcggcc acgctatcgt tcataagcgc cgcaggcctg
    20761 gcgttttgca tcctgtcgaa accggccttt acgacagcgt tcttgtcgtc gtcgtccggg
    20821 gctatgtcga gcaggtctac ttccaggccc gcgttggcgc agtgggcggc gatggcggcg
    20881 cccatcgtgc cggccccgag tatggctacc ttcctgatcc tgtacgacat aaggcctccc
    20941 gatcatatcc gtagagctcc ctgtgcaaag actcgatagc ctctcttacc cttaagaggt
    21001 gggcctcgaa tctgcccttg atctcagcgt acttctcttt gctcgccgcg tcgctctcca
    21061 tcgcctcagc taccctctcg tcgaaaacgt gtcaaaatcg ataataacgc gggtgagaag
    21121 ttttgcgtag aggtctgggt cccgatagag ttgggattcg gagaggctga cagaaggagg
    21181 agttggagtg atcgctgacg tcactcggtc attggggacc gactactatc tgctcgacga
    21241 gctgttcacg gaggaggagc gtgagatccg ggacagggtt cgggctttcg ctgacgccga
    21301 ggtcatcccg attatcaacg agtactggga gaaggccgag ttcccgttcg agctcgtacc
    21361 caaggtcgcc gccctgaaca tagcgggtgg ccctatcgag gagtacgggt gtcctggcat
    21421 gagccaggtc gcagcagggc tcgtcgctat cgagatggcg cgtggggacg ggagcttgaa
    21481 tactttcttc ggggtccatt ccgggcttgc gatggggaca gtggcgatct gcggttccga
    21541 ggagcagaag agaaggtggc tccccgagat ggcgaggatg gagaagatcg gggcctgggg
    21601 cctgacggag ccggatcacg gttctgactc cgtgatgctg gagacaacgg cccaccgcga
    21661 cggggactcc tgggtcttaa acggcgagaa acgttggatt ggcaacgcca cgttcgccga
    21721 cctcgtcata atctgggcgc gcgacgtgga ggatgagcag gtgaaggcct tcgtgctaga
    21781 gaaagacgag aacggcgagc accatccagg ctactccacg gagctgataa agggcaagat
    21841 gggcaagcgc gccgtgtggc agccggatat acatctaaag gatgtccgcg ttcccgacga
    21901 gaaccgcctg gaaggcgcca actcattcaa ggacacgaac aaggtgttga cggccacccg
    21961 cagcggagtc gcctgggagg ccatagggca tgccatcgcg gcttatgaag cggcgctgca
    22021 gtatgcccag gagcgggtac agttcggcaa gccgatagcc ggcttccagc tcattcagaa
    22081 caagctctcc ggcatgctgg cggagatcac caacatgcag ctaatgtgct ttcgcctcgc
    22141 ccagctccag gagcagggta agatgaccgg tcctatggcc tccctggcca agatgaacaa
    22201 cgcacgcaag gcaaagcagg tctgcgccga cgccagggac atcatgggcg gcaacggggt
    22261 cttgctggag taccacgtag cgcgtcacct ctccgacatg gagatcgtct acacgtacga
    22321 gggcaccgac acgatacagt cgctgatagt ggggcgtgac gtgacgggaa tttcggcgtt
    22381 cgtgtagtga agctggtcag cacgctccgg ccttcggcct ccgcttgtca acacttcagc
    22441 atttcagcta agggcattta gggatggcac gtcaccccgg caaccacgct ggtgtagaaa
    22501 gcaaagttgt cgtacgacag catgagaggc gcatccgtac taagctgact agctgaaatg
    22561 ctgactagct gaaatgctaa gcgaccgaag ggagagccca tgaaagccat tcgtctacac
    22621 gaactcgaag ggccagacga gctcgtctac gaggaggtgc cggagccgga gccggggcct
    22681 ggtgagacta tcgtccggct gcgggcggcg gcgctgaacc gccgggatct tttcgccacc
    22741 caggggcagt atcctggggt cacgcctgac attcttccga ttattccggg ctctgatggg
    22801 tcaggcgagg tcgttgcgcg cggagatggc gccgaggggc ctgacgaggg gactggggtg
    22861 gtcatcaacc ctgccctgta ctggggcaac gacccgcggg tgcctggcaa ggagtatcgg
    22921 attctcggta ttccggacga agggactttc gcccagttta tcaaggtgcc ggcggatcac
    22981 gtcttcccca agccctcgca tctttcgcac gaagaggccg ccgcgctacc tcttgccgcg
    23041 ttgaccgcct acagggcgct cgtgacgcgc ggcgggatct ccgagggcga gaccgtactc
    23101 gt
//
LOCUS       DATMVF010000239         9103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_361613, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000239 DATMVF010000000
VERSION     DATMVF010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361613"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1109)
                     /locus_tag="VN178_12285"
     CDS             complement(309..1109)
                     /locus_tag="VN178_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS81793.1"
                     /translation="MATGTTFDVAKMRVHILDDGVFVTDAGNLFGGSRKASIKGAMHP
                     VLVETGDNLVLVDAGFGPELPEILVGRYELRRDSDLLDHLDEAGFSAGDITHVVLSHL
                     DADHVGWALGPPSFPNATVYVQKSALEEARRMPEKDGRRAAVPAVEKGVEDGWCELLD
                     EDGDVLPGIDVEVRSGHSAGHQIVWIGEGDDLALYTGDLAPAKIWLDPDLIAGVDTDP
                     EAARRNRIEVLSAAESRDAPVILYHEPQDCLVKVRSKGDGFEAIPIGD"
     gene            complement(1248..2486)
                     /locus_tag="VN178_12290"
     CDS             complement(1248..2486)
                     /locus_tag="VN178_12290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS81794.1"
                     /translation="MAERYDLVVVGAGPGGTSAAYHSAKAGLNTLLLDRQEFPRDKTC
                     GDGLMPHAASEVALMGLADWLDEPHHGKFTGFSIYTHTAYLRQSVPPTLHGPRGYVVR
                     RKETDARLLERATSAGVQFRSRVRATDLVKSAAGTVSGLEAANAGESLRFETPLVIVA
                     DGVGGFAGKGMKAHQNAVARRQYFSGVEGPDKQDLQVFITEDMNSHGAGYGWVFYLGD
                     GTANVGAGVSTAALARTGRNLKDFFDRFLEHPQLAGWLENATPEGPPTSWSLKMGMWG
                     AKRYDQGLMAVGDAGSMVHPISGEGVGYALESGRLAASWAHEAHARQDFSASLLSGYE
                     RQLKRRRAREHLSGYALVNLVPNLMLLEPLFKVCEKDPEARRMLLEAFTGDAPVYSLL
                     KHPRMLATALRDGVTEKIRS"
     gene            2553..2756
                     /locus_tag="VN178_12295"
     CDS             2553..2756
                     /locus_tag="VN178_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81795.1"
                     /translation="MVDRNRPARYTVGDRAALNATGQPVEILDVRRGEFVPDFVYKVR
                     VLAEKPARPYNLSVPEHDLSDLD"
     gene            complement(2898..3950)
                     /locus_tag="VN178_12300"
     CDS             complement(2898..3950)
                     /locus_tag="VN178_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81796.1"
                     /translation="MSSTIETLERELGDAQTEREAEQRAVVRAENAHEAACSARDEAW
                     SEGSAADPEVEELTDLVYRRRRELYDAIDRLKAAEGKERRIKSRLMYVLPAERRRYLS
                     GTVDEEFEELLAAVREAYSDTLPGARVSVVTERPGEVDLRGDATWRALERLYSGTVRR
                     WVEIRSEVDSPEAGRMARQISYYAGGHTPRTFVDHVEIRAGSFRSPRELRTLAGYESA
                     EGRMALARITARRYLDENSVGRLGKRPRSLVQIAEIARLYQTMSLTVDEIRWRLEQLI
                     SLRFEVLEAVYKLTGQRRRFSFYEIARAARLLDGHFPDAGVAAEVLTQCVRQALSTGV
                     PLVDVAYMNVTTGEGF"
     gene            4137..4787
                     /locus_tag="VN178_12305"
     CDS             4137..4787
                     /locus_tag="VN178_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HWS81797.1"
                     /translation="MDFNTESGGSGGSSGPPRVSGGTSGGEFDYSDPVQSFINAVRGV
                     VTAPVGFFRGIRHQGDFVNPLIFAIICYEVSAVLGGLLGLVGVSMGGTQGFGAFLVSL
                     VVAPIFAAVGLFIGAGILHLLVMLIVGSRNSGFEGTFRVGAYSSVTSLVSWIPVIGWI
                     ASLYGIYLAIMGIREVHNTSTGKAVIVVLIPAVVVFLLALLIIAAVGAALFFGTQQ"
     gene            4878..5882
                     /locus_tag="VN178_12310"
     CDS             4878..5882
                     /locus_tag="VN178_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HWS81798.1"
                     /translation="MAQTVTLIPGDGIGPDLTDSVKEVIGALDVDVEWEIAEAGETVM
                     DSEGTPLPESVLDSIQRNKVALKGPLTTPVGTGFRSVNVALRKELDLYGNIRPSLSLP
                     GVETPYQNIDIIIYRENTEDLYAGVEHTVGKHAAESIKIITREGTERICRMAFERSKE
                     QGRKHITVVHKANIMKFTDGLFRDVFFEVARDYENDFEEIDDRIVDNMCMQLVMKPNL
                     YDVLVCPNLYGDILSDLCAGLTGGLGVAPGANIGEEIAVFEPVHGSAPKYAGQNRANP
                     LATLLSAKNMLIHLDYSGEADRMQAGIEAALKSPETRTKDLGGSAGTREFTQAIVSNL
                     "
     gene            6014..7135
                     /locus_tag="VN178_12315"
     CDS             6014..7135
                     /locus_tag="VN178_12315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWS81799.1"
                     /translation="MRIAFFTETFLPATDGVVTRLRHTIEELLGMGDEMLVIAPKYGE
                     GPGSYEGVPIHRVSSVPLPPYPQIKLAPINPGVGTALRGFGPQLIHAVNPFVLGGGAP
                     FYSRRLGVPLVASYHTNIAAYARFYRLGLFDRATRWYTRKLHNRAAINLCTSEATLRY
                     LAGEGVERLHLWPQGVDARRFHPDKFSKDWRVRLTNGHPAERLLLYVGRLGHEKDIGK
                     LKVVLGEEPGVRLAIVGDGPARRDLEQEYANTPTVFTGVLQGEELASAFASSDVFLFP
                     STTETLGIAMIEALASGLPVLAARAGAAGEVVDDGETGLLYVPGSDASLVAAVRKIVA
                     DDDLRARMGRKARASAERRDWESSTRTLRGYYERALEER"
     gene            7132..7596
                     /locus_tag="VN178_12320"
     CDS             7132..7596
                     /locus_tag="VN178_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWS81800.1"
                     /translation="MSEAKAKLKSGGKRFSKFSLVGISNAAVDIGVLNLFLWLAPTRE
                     VTLLVIYNGVALILANLNSYAWNTLWTFRGRAKHDTRQIVLFGLQVLVNIGISNGLFW
                     ALIRPIIVYTEVPTYLAGNVAKIISVIVASTISFFIMRYVVFSRKRWFNGRL"
     gene            7630..7702
                     /locus_tag="VN178_12325"
     tRNA            7630..7702
                     /locus_tag="VN178_12325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7663..7665,aa:Gly,seq:gcc)
     gene            7722..7792
                     /locus_tag="VN178_12330"
     tRNA            7722..7792
                     /locus_tag="VN178_12330"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7754..7756,aa:Cys,seq:gca)
     gene            7815..7886
                     /locus_tag="VN178_12335"
     tRNA            7815..7886
                     /locus_tag="VN178_12335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7847..7849,aa:Val,seq:gac)
     gene            8133..8633
                     /locus_tag="VN178_12340"
     CDS             8133..8633
                     /locus_tag="VN178_12340"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HWS81801.1"
                     /translation="MADDRLQQKLVGYVEDAHAMERNVSTMLDSMISTTDDPEIKRML
                     EDHKRQTQEHERRLRERLDAMGASTSTIKEVGGVGAALFKGVGDMARSDKHAKNGRDG
                     YTTEHMEIAAYELLERLAKRAGDEKTAEVARQNRSDEREMAKKIESNWDRFLDLTLAT
                     PGFLAE"
BASE COUNT         1655 a         2827 c         2911 g         1710 t
ORIGIN      
        1 aagacaatac tcagacgcgc cctcataatt gccgcgccga ttgtatggcg caagatcagg
       61 aaccgcaggc gcgaacgctg atcagcactt cagcacgctc cggccattca ggccttcgct
      121 tgtcagcaag tcagcttctt ccgtttcgga ccttgctctg ccgcgcccga cgaaccgcta
      181 ccagcggcag tcgggtacta tgctcccggc gaggatatac gggacgactt gctgaaatgc
      241 tgataagcga aggctaaaag cctgagcgtg ctgacaaggc gcgaagcgcc gtgctgaagt
      301 gcttatgctc aatcccctat cgggatggct tcgaagccgt ctcccttgct gcggaccttg
      361 acgaggcagt cctgtggctc atggtagagg atgacggggg cgtcgcgtga ctctgcggcc
      421 gagagcactt cgatgcggtt gcggcgggcc gcttccggat ctgtatccac gccggcgatg
      481 agatcggggt cgagccagat cttggccggc gcgaggtctc ctgtgtagag cgcgaggtcg
      541 tctccctccc cgatccagac gatctgatgc cccgcagaat gccccgaacg cacctcgacg
      601 tcaatgcccg gcaggacgtc tccgtcttcg tcgagcagct cgcaccagcc atcttccacg
      661 cccttctcga cggcggggac cgccgccctg cgcccgtcct tctccggcat cctccgcgcc
      721 tcctccagcg cggacttctg cacgtagacc gtggcgttgg ggaagctcgg aggtcccaac
      781 gcccagccga cgtggtcggc gtccaggtgc gaaaggacga cgtgcgttat gtcccccgcg
      841 gagaagcccg cttcgtcgag atggtcgagg agatccgaat cccgtctcag ctcgtagcgg
      901 ccgaccagga tctctggcag ctctggtccg aacccggcgt cgacgagcac gaggttatcg
      961 cccgtctcga cgaggacggg gtgcatggcg cccttgatgc tggccttccg agagccgcca
     1021 aagaggttgc cagcgtcggt aacgaacacg ccgtcgtcca gaatgtggac ccgcatcttt
     1081 gccacgtcga aggttgtccc ggtagccaag tcgccgaacc ctcctctagt cgtgaatctg
     1141 aatgatagcg ggcccggcga ctcgatgctc tgttagtgga tgcggcccgc agggatctgc
     1201 ggcgtaaggg gatggactca ggaggagcta cccggacgac ttggtcttta tgaccggatc
     1261 ttctccgtga cgccatccct tagtgctgtc gccagcatgc ggggatgttt gaggaggctg
     1321 tagacgggtg cgtcgcccgt aaacgcctcg aggagcatcc ggcgggcctc ggggtccttc
     1381 tcgcagacct tgaagagggg ctctagcagc atcaggttcg gcacgaggtt gaccagtgcg
     1441 tatccagaga ggtgctcgcg ggcccgcctg cgcttgagct ggcgttcgta gccggagagg
     1501 agcgaggcgg agaaatcctg cctggcatgt gcctcgtggg cccacgaggc ggcgaggcgg
     1561 cccgactcca gcgcgtaccc gacgccctcc ccgcttatgg ggtggaccat gctgcctgcg
     1621 tccccgaccg ccatcagccc ctggtcgtag cgtttcgcgc cccacatccc catcttgagc
     1681 gaccagctcg tcggcggacc ttccggtgtg gcgttctcca gccagcctgc aagctgcgga
     1741 tgttcgagaa agcggtcgaa gaaatccttg aggtttcggc ccgtgcgggc tagcgccgct
     1801 gtcgagacgc cagccccgac gttcgccgtg ccgtctccca ggtagaagac ccaaccgtag
     1861 cccgcgccgt gcgagttcat gtcctccgta ataaagacct gcagatcctg cttgtcaggg
     1921 ccctccacac cgctgaagta ttgcctgcgg gcgacggcat tctggtgagc cttcataccc
     1981 ttacccgcga agcccccgac gccgtcggcg acgattacca acggggtttc gaagcgcaag
     2041 gactcgcctg cgttggccgc ttcgaggcca ctcactgtgc cagccgccga tttcacgagg
     2101 tcggtagccc tgaccctgga acggaattgg acgccggcgg aggtcgcacg ctcgaggagc
     2161 ctggcgtcgg tctccttgcg ccttacgacg tagccccgag ggccgtgcag ggtagggggg
     2221 acgctctggc gcaggtaggc ggtgtgggtg tatatggaaa agccagtgaa cttgccgtgg
     2281 tgcggctcgt cgagccagtc ggcaagcccc atcagcgcga cctccgaggc ggcgtgcggc
     2341 atcaggccgt cgccacaggt cttgtccctg gggaactctt gcctgtcgag tagcagtgtg
     2401 ttcagccccg ccttcgcgga gtggtaggcc gctgaggtac caccaggacc ggctccaacg
     2461 accacgagat cgtaccgttc ggccatcgtc ctccttacct gagccctcac taatgtgcca
     2521 gaattatact tctgctacga cgaggaggaa acgtggtcga ccgcaacagg cctgcgcgct
     2581 acacagtcgg ggatcgcgcg gccctgaacg ctaccggaca gccggtcgag atactcgacg
     2641 tgaggcgggg cgagttcgtt cccgacttcg tctacaaggt tcgcgttctc gcagagaagc
     2701 cagcacggcc ctacaatctt tcggtacccg aacatgatct ttcagacctg gattaaggct
     2761 tcaggctaca ggcatcaggt aggagagggc cggagaggct gcttcggggt atccgaacct
     2821 ggggtcagag gccacattct ggtcggcgag ttagtgagag gtcgggtgcc atcgagcagt
     2881 cctgaaacct gatgcctcta aaagccctcg cccgtagtga cgttcatgta ggctacgtcg
     2941 acgaggggga cgccggtcga gagggcctgt cgtacgcact gggtgagaac ttcggcggcg
     3001 acgcccgcgt cggggaagtg gccgtcgagg agacgggcgg cgcgggctat ctcgtagaat
     3061 gagaaacggc gacgttggcc ggtcaactta tagaccgcct cgagcacctc gaagcgcagg
     3121 gagatcagct gctccagcct ccagcggatc tcatccacgg tgaggctcat ggtctgatac
     3181 aggcgggcga tctcggcgat ctggacgagg ctccgggggc gcttgccgag gcgtccgact
     3241 gagttttcgt cgaggtagcg ccgcgctgtg atgcgcgcca gcgccattcg tccctcagca
     3301 ctctcgtagc cggccagggt cctaagctct cgcgggctcc tgaaggagcc cgctcttatc
     3361 tccacgtggt cgacgaaagt gcgcggggta tggccgcccg cgtagtagga gatctggcgc
     3421 gccatccttc ctgcctcggg cgagtcgacc tcgctcctta tctccaccca cctgcgcacc
     3481 gtgcccgagt acaaacgctc cagtgcccgc cacgtcgcgt ccccacgcag gtccacctcg
     3541 cccggtctct cggtaaccac ggagacccgc gcgccgggca gagtgtcgct gtaagcttcc
     3601 ctgacggccg caagcagttc ttcgaactcc tcgtcgacgg tccctgacag gtacctgcgc
     3661 cgctcggcag ggagcacgta catgagccgg ctcttgatcc tgcgctcctt gccctccgcg
     3721 gccttgagcc ggtctatggc atcgtagagc tccctccgcc ggcggtagac cagatcggtc
     3781 aactcctcca cctcgggatc ggccgcagag ccctcggacc aggcctcatc ccgcgccgaa
     3841 cacgccgcct cgtgggcgtt ctcggccctg acaacggccc gttgctccgc ttctcgttcc
     3901 gtctgggcgt cgcccagttc ccgctcgagg gtctctatcg tcgaggacat aaacagttcc
     3961 cgttcccgaa gacgcacgac tccacgctta ttctacagta ccccgcatgc ccacgacgct
     4021 ccctgaacga ctcgctccag aacgccgcac gttgcccaca ggcgcggttg cgcgggttaa
     4081 ctggaacgtg tacattacta ctagttatcc gaagtgcgat gggagaggag attcgcgtgg
     4141 acttcaacac ggaatctggc ggtagcgggg ggtcgagcgg gccgccccgg gtatctggtg
     4201 gcacttcggg tggggagttc gactacagcg atcccgtcca gagcttcatc aacgcggtta
     4261 ggggcgtcgt aaccgcgccc gtcggctttt tccgtggcat caggcaccag ggagacttcg
     4321 taaacccatt gatcttcgcg atcatctgtt atgaggtttc ggcggtcctg ggtggcttgc
     4381 tcgggcttgt gggtgttagc atgggcggca cccaggggtt cggtgctttc ctcgtttcgt
     4441 tggtagtcgc cccgatcttc gccgccgtcg gcctgttcat cggggccggc atactgcacc
     4501 tgctcgtgat gttgatcgtc ggctcgcgca actccggctt cgagggcacg ttcagggtag
     4561 gcgcttactc ttccgtgacc agcctcgtga gttggatacc cgttatcggg tggatagcca
     4621 gcctgtacgg tatctacctg gccataatgg ggatcaggga ggttcacaac accagcacgg
     4681 gtaaggcagt aatcgtggtc ctaatacccg ctgtggtggt gttcctgctc gccctgttga
     4741 tcatcgccgc tgtcggggcc gctctcttct tcggcaccca gcagtaacga gcctccggta
     4801 cggtccgtag tcgggcatag ctgccctcac gccaggccgc tatactatcg gcggtctaca
     4861 gcgaaggggg tttgcgcgtg gcgcagacgg ttactctcat accgggcgac gggattggcc
     4921 cggatctcac ggactccgta aaggaggtca ttggcgcgct cgacgtcgac gtcgagtggg
     4981 agatcgccga agctggcgag acggtgatgg acagcgaagg tacgcctctt cctgagtccg
     5041 tgctcgactc tatccagcgc aacaaggtgg cgctgaaggg gccgctaacc acgcccgtgg
     5101 gaacggggtt caggtccgtg aacgtggcgc tgcgcaagga gctcgacctc tacggcaata
     5161 tcaggccttc cttgagcctg ccaggggtag agacgccgta ccagaacatc gacatcatca
     5221 tctaccgcga gaacaccgag gacctctacg ccggtgtgga gcacacggtc ggcaaacacg
     5281 cggcggagtc gatcaagatc atcacccgcg agggcacgga gcgcatctgc cggatggcct
     5341 ttgagcgctc taaagagcag ggccgcaagc acatcacggt cgtccacaag gccaacatca
     5401 tgaagttcac ggacggcctg ttccgtgacg tcttcttcga agtcgccagg gactacgaga
     5461 acgacttcga agagatagac gaccgcatcg tcgacaacat gtgcatgcag ctcgtcatga
     5521 agccgaacct ctacgacgtt ctggtctgcc ccaacctcta cggcgacatc ctgtctgacc
     5581 tctgtgcggg acttacgggc gggctcggcg tcgcccctgg ggccaacata ggggaggaga
     5641 tcgcggtctt cgagcccgtc cacggttccg ctccgaagta cgccgggcag aacagggcca
     5701 acccgctcgc gacgctgctg tccgccaaga acatgctgat ccacctcgac tacagcgggg
     5761 aggccgacag gatgcaggcg gggatagagg cggccctgaa gagccccgag acgcgtacaa
     5821 aggatctagg cggcagcgcc gggaccaggg aattcacgca ggccatagtc tcgaacctgt
     5881 agagtcgggg tagggatcgt tacgagcaga cctaggaggg tggtgttggc cggtagcgag
     5941 aggacctgta ggggagcggg ttggcggcga tacggcgcgg taccggccgg gtcagagcgc
     6001 tgctagcggt cctgtgcgca tcgcgttctt taccgagacc ttccttccgg ccaccgacgg
     6061 tgtggtcacg aggctcaggc acaccataga agagttgctc gggatgggcg acgagatgct
     6121 cgtgatcgcc cccaagtacg gagaaggccc gggttcctac gagggggtcc cgatccaccg
     6181 cgtctccagc gtgcctctcc cgccctatcc ccagataaag cttgcgccca taaaccctgg
     6241 tgtgggcacc gctctgcgcg gcttcggacc ccagctgatc catgccgtca accctttcgt
     6301 gctcgggggg ggagctcctt tctactccag gcgcctgggc gtccctctgg tggcttccta
     6361 ccacacgaac atcgccgcct acgcccgctt ctaccggctc gggctcttcg accgcgccac
     6421 tcgctggtat accaggaagc tgcacaacag ggccgccatc aacctctgca cctctgaggc
     6481 gacgctccgg tacctcgccg gggagggcgt ggagcggctg cacctgtggc cgcagggtgt
     6541 cgacgctagg cgcttccacc ccgacaagtt ctcgaaggat tggcgggtca ggctgacgaa
     6601 cggtcacccg gctgagcggc ttctgctcta tgtaggacgc ctcggtcacg agaaggacat
     6661 cggtaaactc aaagtcgttc tcggcgaaga gcccggagtg cgtctcgcca tcgtcggaga
     6721 cggacccgcc cgcagggacc tcgaacagga gtacgcgaat acgccgacgg tctttacggg
     6781 ggtgttgcaa ggcgaggagc tcgcgtccgc gttcgcctcc tccgacgttt tcttgttccc
     6841 ttcgaccacg gagacgctgg ggatagcgat gatcgaagcc ctcgcctccg ggctgcccgt
     6901 gctcgccgcc agggcgggtg cggccgggga ggtcgtggac gatggcgaga cgggacttct
     6961 ctacgttcct ggttccgacg caagcctcgt cgccgccgtc agaaagatcg tcgcggacga
     7021 cgacctgcgg gcacggatgg ggcgtaaagc ccgcgcgtcc gctgagagac gagattggga
     7081 atcttcgaca cgcacgctgc gcgggtatta cgaacgggcc ctggaggaga gatgagcgag
     7141 gccaaggcca agctaaagag cgggggaaag cgcttctcca agttctccct cgtcggaatc
     7201 tccaacgctg ccgtagatat cggggttttg aacctcttct tgtggctggc gccgactcgc
     7261 gaggtgaccc tgctcgttat ttacaacggc gtcgcgctca ttcttgcgaa cctgaacagc
     7321 tacgcctgga acacgttgtg gacctttcgg ggtagggcca aacacgatac gaggcagata
     7381 gtcctgttcg gcctacaggt gctcgtcaac atcgggataa gcaacgggct gttctgggcc
     7441 ctgatccggc ccatcatcgt gtatacggag gtgccgacat atctcgccgg caacgtggcc
     7501 aagatcatct ccgttatcgt ggcctctacc ataagttttt tcatcatgcg ctacgttgtc
     7561 ttctccagaa agcggtggtt caacggacgc ttgtaagagc cttgggcccg tgctaaatta
     7621 gggccgcgtg cggaagtagc tcagttggta gagcatcacc ttgccatggt gagggtcgcg
     7681 ggttcgaatc ccgtcttccg ctctggccgt agggcgccgc tggcggcatg gccgagtggt
     7741 taggcaaggg tctgcaaaac cctgtacccc ggttcgattc cgggtgccgc ctctcggagt
     7801 ggcgaagtaa gtacgggcga ttagctcagg gggagagcgc ttccttgacg cggaagaggc
     7861 cagtggttcg aatccactat cgcccactct gaaaggccct gcaaataccg agaaaaacac
     7921 gcgccttgga ttcttgcgcc ggggactttg cgcatcaacc cgcgttgagg gggttctcgg
     7981 aagcgcgtcc gctgcccgtc ctcggacggt gagccgaagg gtttaaccga agtctcgcga
     8041 tctcgccacg ctgcccgagc gttttctggg cggcgcctgc gggtatccat gccagaggaa
     8101 atggatcggg gtccgaccgg gaggtagtag gcatggcaga cgacaggctc caacagaagt
     8161 tggtcggcta cgtggaggac gcccacgcca tggagcgcaa tgtctccacg atgctcgact
     8221 cgatgatctc caccaccgac gaccccgaga tcaagcggat gctcgaggac cacaagcgcc
     8281 agacccaaga gcacgagcgg aggctgcgcg agcgcctcga tgccatgggg gcgtccacct
     8341 cgacgatcaa ggaggtcggg ggggtgggcg ccgcgctctt caagggcgtg ggcgacatgg
     8401 cccgttccga caagcacgcc aagaacggcc gcgacggcta cactaccgag cacatggaga
     8461 tagccgccta cgagctccta gagcgcctgg ccaagagggc gggcgacgaa aagacggcgg
     8521 aggttgcgcg ccaaaaccgc tcagacgagc gggagatggc caagaagata gagtccaact
     8581 gggacaggtt cctggacctc acgctggcga cgccgggctt cctggccgag taggcagcgc
     8641 gtccacatct ccggtcgggg ccgttacgca tcggccctcc tttgcttcct attcaacgga
     8701 gtgcgtggaa gaaaaattct cggaaactca gcttccgatg ttaggcgttt tcggaaactg
     8761 agcagcacgc tggtcgcata gacttacgac agcttgcgta agcggtgagc tcgtgccaga
     8821 ctgctagcag cgagcagagc ggccgagcag agtgggcaag ctatgctcga tagataccaa
     8881 gcaacgcgcg ggtaaactgg tcggaggtcg aaggtgggca ccttcttcaa ggacgatgat
     8941 ttcgagtata tgatgctcgg tgccctagat gtgtgttgtc aggatcatgc ccacagctcc
     9001 ttgatgcgcc cttgaggacg tcccagcttc cggttgacca cgaaggcata tgtgtaggct
     9061 gctatcttgg ccgcgatcct ggtggccagc ccagtgagcg tgg
//
LOCUS       DATMVF010000240         8316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_535758, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000240 DATMVF010000000
VERSION     DATMVF010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535758"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VN178_12345"
     CDS             complement(<1..340)
                     /locus_tag="VN178_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81802.1"
                     /translation="MATSDEDVNRQYDEDSHVTGQQWKWTVLAGMASYLDAGSIVALA
                     AGLALWQEYLGMSSTTVGLLAALGPNAFGAAIGALIGGRLGDLFGRKRIYQYDLLVYG
                     LGTLLIVFSLN"
     gene            complement(427..1212)
                     /locus_tag="VN178_12350"
     CDS             complement(427..1212)
                     /locus_tag="VN178_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HWS81803.1"
                     /translation="MSEKRADLPEQRQQRIAMLVREVGSVTVAGLEKELGISAATARR
                     DLTLLERRGKVKRTHGGAVAPGLTQHEDSFQQRLGEAVEPKRRLARAAAALLEADETV
                     FIDSSTTAYYAARRILAGTPGVTCLTNLVPVMDLFSTVDPSGASMVGLGGIFRALTLS
                     FVGPCTIQTIESYIADRALISVKGITSQGYLTDIHPLEAEVKRAMIRQSERPVLLVDG
                     RKFEQRGSSVIAHVSEVSLVVTADARRPHVEALGDLGVDVRSV"
     gene            1695..2249
                     /locus_tag="VN178_12355"
     CDS             1695..2249
                     /locus_tag="VN178_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS81804.1"
                     /translation="MGKLIATTQATVDGVIDPVGEWVQADGDHAEYSFERQARSSGLV
                     LGRKTYEGLAGYWPSQSGKWADMVNPLPKYVGSNTLSGDLEWNATLLAGDLGDSIPKL
                     KDEVDGDLFMHGSGEFAYALAERGLIDEYEVYLNPLVWGKGNVHVLGDRGTIWMELAD
                     VTRFDSGVVLLTYHNRASSRRRAD"
     gene            2422..2862
                     /locus_tag="VN178_12360"
     CDS             2422..2862
                     /locus_tag="VN178_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS81805.1"
                     /translation="MTLLRMDNVLIVVDDLKAAIAFFAELGLELEGETTVEGQSVDRL
                     VGLDGVRSDIALMRTPDGQGRIELDKFHTPTAVRAEPEDAPVNALGIRRIMFAVDDIE
                     DVLARLRTHGATLVGEVTQYEDTYRLCYVRGPEGIMVALGEQLS"
     gene            3118..3459
                     /locus_tag="VN178_12365"
     CDS             3118..3459
                     /locus_tag="VN178_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81806.1"
                     /translation="MSENQMVALTIDTEVHPPKSLLIRGRAGLDFVDGIPDEYLQVTS
                     TYEMTPEQRVEWEAEVRSLYHDGMVRIVVTPTWAKLIDFESTLPSAGRARLIRSHVYR
                     VTEQTSGRMSS"
     gene            3668..4513
                     /locus_tag="VN178_12370"
     CDS             3668..4513
                     /locus_tag="VN178_12370"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HWS81807.1"
                     /translation="MRSCAPEGHSFIMVPEPGGVRLRPSGGEWTVVREGLTRRQGGED
                     GFRWRGEDVSRIEGFSDAVFAFAVTLLVVSLEVPKTFDELLATMRGFFAFAICFYLLL
                     IVWYEHYKFFRRYGLRDLLIQFLNGAMLFLVLMYVYPLKFLFTLLIDQLFGFAENETI
                     EPSQWPQLMVIYGAGFVAIQLVFVLMYLRAHSLRATLELDASELSMTREEIQGSLLNM
                     SVGLASVAIAFLGGERLLPWAGYVYVLVFPLQVINSRVMGSRRKKESSSHTQATGEDA
                     DGEGP"
     gene            4592..5326
                     /locus_tag="VN178_12375"
     CDS             4592..5326
                     /locus_tag="VN178_12375"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWS81808.1"
                     /translation="MEQSPRTRLRDRAADQLLEMVISDGLKPGERLPPERELCELLGV
                     SRTVVREALNLLEARGLISIEHGRGAVVSGGNPRAVRDTLGLLLRVQPKTLWELLEIR
                     RILEVEVAGLAAERAGPDDVTAMRLQLDRMRDSIDAPEGYVDADVEFHALLARATRNE
                     VLLTMLEPVVDLLRASRRVSAARPGNALRALGEHEAILRRVEAKDAGGARGKMRAHLA
                     KTAEDIEAAIGEGVLIAGAFSEKEEV"
     gene            5323..6270
                     /locus_tag="VN178_12380"
     CDS             5323..6270
                     /locus_tag="VN178_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HWS81809.1"
                     /translation="MKAHEMLSELGLPGRDLYGPPVSGKRFPDGAQYRVEIPSVEGPR
                     VLEAVIEEADRREVQIHRVSQGSGIMLLTDAEILEMCHMARESALELSLFVGPRASWE
                     TGATAVSGAGKVLGAQHRGQDQLAYAIEDVLRGVSLGLRGVLVADAGLLWVLRDLKAK
                     GELPEDLVLKVSVQLAAANAAAVKAMEDLGADTYNVPTDLSLAQLAAIRDTVDLPLDV
                     YVEVPDDFGGFVRHYEIPDLVRVASPVFVKFGLRNAPNIYPSGTHLEATAVALGRERV
                     RRARIGQEMLARYYPEAETTPPGATFPGLPVDAKVKEKA"
     gene            6267..7427
                     /locus_tag="VN178_12385"
     CDS             6267..7427
                     /locus_tag="VN178_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWS81810.1"
                     /translation="MKVTHVETFVVGTEWRNLTIIRAHTDEGLTGLGEARMVNHTDAL
                     VGYLAEAAPRHLVGHDPFRIEELVKKMGRDDFSRAGEVMSSGIAMFETACWDIVGKAL
                     GQPVYNLLGGPVRERVKAYANGWYRVERTPEEFHEAAKKVVERGYRALKLDPFGPGHY
                     ELEYEEKIKAVSLVEAVRDAIGPEVEILLEMHGRFSPATAIEMARLLQPFGLGWVEEP
                     VPPVNLKALKKASDGIEATVATGERIHQRHEYREVFELQAADVIQPDISHIGGLLETK
                     KLAAWADAYYVTIAPHNVGGQINTAAALHLAACTTNFRIQEYFNDFADPWVRETAPGL
                     SEVRDGYFELPTGPGLGVELDEEVIEAHPKQDVHFNLFAEGWERREGAETNQ"
     gene            7424..8203
                     /locus_tag="VN178_12390"
     CDS             7424..8203
                     /locus_tag="VN178_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWS81811.1"
                     /translation="MSDLSGKKVLVTGAGMGIGQGIAVELARRGAEVAVHYAHTEPEE
                     TVSRIKQLGARAATVQGDLSKVEACERTVDAAAEALGGLDTLVNNAGVTKTLDFLDTD
                     EETYDSVFDLNMKGYFFLARKAVPLMVERGGGSIVNVTSVHGFAGFPNHTAYAATKGA
                     INAFTRQLAIELTDRKVRVNAVGPGIIEVPRYFDIPNYTTEFGDTLVPWGRVGYPEDV
                     APAVAFLVSDAADFVTGQVLYVDGGTTARMGLWWDQGDGPE"
BASE COUNT         1582 a         2624 c         2677 g         1433 t
ORIGIN      
        1 ggttgagcga gaagacgata agcaaggtcc ccaagccgta gacgagcaga tcgtattggt
       61 agatgcgctt gcggccgaac aggtcgccga gtcgcccccc gatcagggct ccgatcgccg
      121 ctccgaaggc gttcgggccc agggctgcga gcagtcctac ggtggtgctg ctcatgccga
      181 gatactcctg ccacagcgcg agtcccgcgg cgagggccac gatggaccca gcgtccaggt
      241 atgaggccat gcccgccagg accgtccact tccactgctg gcccgtgacg tggctgtcct
      301 cgtcgtattg cctgtttaca tcctcatccg aagtagccat gattcaggtg agccctcccc
      361 ctgctcgtcg cttccgtcca acatacccta tttccgtccc aacgctcact ctttcgcctc
      421 cccttttcat acgctccgca cgtcgacccc gaggtccccg agagcctcca cgtgcgggcg
      481 acgggcgtcg gccgtcacca ccaggctcac ctccgagacg tgggcgatga cgctggagcc
      541 ccgctgctcg aacttcctgc cgtcgacgag caacacgggc ctctcggact gccggatcat
      601 cgccctcttg acctcggctt cgagcgggtg gatatccgtg aggtacccct gagacgtgat
      661 ccccttgacg cttatcaggg ccctgtccgc gatgtatgac tcgatggtct gaatcgtgca
      721 cggccccacg aacgagaggg tcagcgcacg aaagatcccc ccaagcccta ccatactcgc
      781 cccagaggga tcgaccgtcg agaagagatc cattaccggc accaggttgg tcaggcaggt
      841 gacacctggg gtgccggcca atatacggcg cgccgcgtag tacgccgtgg tcgacgagtc
      901 gataaacacc gtctcatccg cctcgagcag cgccgctgct gcacgcgcca gacgccgttt
      961 cggctcgacc gcctcaccca accgctgctg gaaggagtcc tcgtgctgcg ttagcccggg
     1021 cgcaacagcc ccaccatggg tcctcttgac cttcccccga cgctcgagca acgtaagatc
     1081 gcgcctcgca gtagccgccg agatgccaag ctctttctcc agcccggcta ccgtgacact
     1141 ccccacctcg cgaaccagca tcgctatccg ctgctgacgc tgctcaggta aatctgcgcg
     1201 tttttccgac atattatgat tgatcgagca gtttttcgtc caaaccgctc aaatccttcc
     1261 ctagctcgcg catacagtaa cggaggcttc acatcgatgc aaccctggtg ctacacgccg
     1321 gggtggtgct cggttcaagc ctcgctgcaa tgtccgggac gcatggcggg accaggaacg
     1381 gccacgaaag ccaggttaag gcgtccttct tcacccgcgg tgaaggtggg tgtggggcga
     1441 ggttcaggcg gctcgggtga cattcggcat gccgatccca cgacgatcat cactaccggg
     1501 cgccgaaatt cgcctttaga ctcgtccgtg aagggaaaac tgaatggaaa acaaggctaa
     1561 agggtgcaac gaaaattgtc cgtgaggctc gagaaaagtt ccctaccccc ttcgaaatct
     1621 accttttggt ggatgtctct ctacccggtc tcgcaatact atcgtcaagg caaaccgatg
     1681 gaaggagaat cgagatggga aagctgatcg ccaccacgca ggccaccgtc gacggcgtca
     1741 tagacccggt cggcgagtgg gtgcaggcgg acggagacca cgccgaatac tcgttcgagc
     1801 gtcaggcgag atccagcggt ctggtgctgg gtcgcaagac ctacgagggt ctggcgggat
     1861 actggccgag ccagtcgggc aagtgggcgg acatggtcaa cccgctgccg aagtacgttg
     1921 ggtccaacac cctctccgga gatctcgagt ggaacgcgac cctgctcgcg ggggacctcg
     1981 gggactcgat cccgaagctg aaggacgagg tcgacggtga cctgttcatg cacgggagcg
     2041 gcgagttcgc gtacgccctc gccgagaggg gtctgatcga cgagtacgag gtgtacctga
     2101 atccgctcgt gtggggcaag ggaaacgtcc acgtgctcgg cgacagggga accatttgga
     2161 tggagctcgc ggacgtgacg cgcttcgact ccggagtggt cctgctcacc taccacaacc
     2221 gcgcgtcgag ccggcgtcga gccgactgaa cgcccgtaag cggatcggct tgcgtgcggg
     2281 ttctcgttcg gcggtgtggc gccccaggac cgtgacggcc agaagcaggt cctacttccg
     2341 gctgtcgaga atgcggcgct ggctccgtcc taggtacacc agtggccacg ccaggccgcg
     2401 cgacgaagaa ggagaactac catgacactt ctgcggatgg acaacgtcct catcgttgtc
     2461 gacgatctca aggctgccat cgcgttcttc gctgaacttg gtctggaact tgagggcgag
     2521 acgaccgtcg agggacagtc ggtggaccgc ctcgtcgggc tcgacggcgt ccgaagcgac
     2581 atcgcattga tgcggactcc ggacggccaa gggcggattg agctggacaa gttccacacg
     2641 ccaacggcgg tcagggctga gccagaagac gcaccggtga acgcgctggg catccgtcgc
     2701 atcatgttcg ccgtcgacga catcgaggac gtccttgctc gcctgcgcac ccacggcgcc
     2761 acgctcgtcg gcgaggtgac tcagtacgag gacacctatc ggctctgcta cgttcgcggc
     2821 cccgagggga tcatggtcgc gttgggtgag cagctcagct aacggcctga cctgcgacca
     2881 accagaacga tccgcttgga tggaccgaga acgacaacgg gaggaataac cgtgcaaccg
     2941 aacgagatcg ccgaggttct gaaccgaccg atcagtcagg aactgttggc ccgcgacgtg
     3001 acccgcttgg cctaagtcgc caaggacgac acaccccgca atgtcccgat catctttacc
     3061 tggaacggct cgcagatcgt catgtgcact tcgaagaacg ctccgaagct tcaggccttg
     3121 agcgagaacc agatggttgc cctgacgatc gacaccgaag tgcacccccc taagagcttg
     3181 ctcatccggg gtcgggccgg gctggacttc gtcgatggca tcccggacga gtatctccag
     3241 gtgaccagca cctacgagat gacgcccgag caacgggtcg agtgggaggc ggaggtgcgt
     3301 tcgctctacc acgatggcat ggtccggatc gtcgtaaccc cgacgtgggc gaagctgatc
     3361 gacttcgagt cgactctgcc gagcgcagga cgcgcccgtc tcatccgctc gcatgtctac
     3421 cgggtaaccg aacagaccag cggtcggatg agctcgtagt ccctctgctc gggcctcccg
     3481 cacaaaagct cgacctgctc ccattcgtcg gtcggctcgg cgcgccggca gcgctgactg
     3541 ctccccattc tcccccctcg tccctcttcg ccgcgggtga agaaggatgc cacaaaccgg
     3601 cttcagcggc cagcttaagt cccgctatgc gtcccagaca ttttacggcg aggtttcctc
     3661 ggttagggtg cgatcctgtg cgcccgaggg tcactccttt atcatggtgc ccgagccggg
     3721 tggagtccgg ctgaggccta gcgggggtga atggacggtg gtgcgggaag ggttgacgcg
     3781 gaggcagggc ggtgaggacg gcttccgctg gcgcggggag gatgtctcac gcatcgaggg
     3841 gttctcggac gccgtcttcg ccttcgcggt aacgctcctg gtggtgtccc tggaggtccc
     3901 gaagaccttc gacgagttgc tcgcgaccat gcggggcttc ttcgcgttcg cgatctgctt
     3961 ctacctgctt ctcatagttt ggtacgagca ctacaagttc ttcaggcgct acgggctgag
     4021 ggatctcctg atccagttcc tgaacggggc gatgctcttc ctcgttctca tgtacgtcta
     4081 ccccttgaaa ttcctcttca ccctgctgat agaccaactg ttcggcttcg ccgagaacga
     4141 gactatagaa ccgtcccagt ggccgcagtt aatggtcatc tacggcgccg gcttcgtcgc
     4201 catccagctc gtgttcgtgc tgatgtacct gcgggcccac tcgctgcgcg ctaccctgga
     4261 actcgacgca agcgagctgt cgatgacgcg cgaggagatc cagggttccc tcctgaacat
     4321 gtcggtcggt ctggcctcgg tggccatcgc tttcttgggc ggggaaaggc tactcccgtg
     4381 ggcaggctac gtctacgtgc tggtcttccc actgcaggtg atcaacagcc gcgttatggg
     4441 ctcccgcagg aagaaggaga gttcgtccca cactcaggcc acaggagaag atgcggacgg
     4501 cgaaggacct taactacccg gtagggaagt agtgccccgg acggtcctcg tttgacaggc
     4561 atcctgattg gtatgacaat gctttgggga tatggagcaa tcaccacgaa ccaggctcag
     4621 ggaccgggcg gcggaccagt tgctggagat ggttatctcg gacgggctaa agccgggaga
     4681 gcgtcttccg cccgagcgtg agctgtgtga acttctcggg gtgagcagga cggtggtaag
     4741 ggaggctctg aacctgctcg aggcccgcgg cttgatcagc atcgagcacg gccgcggcgc
     4801 cgtggtcagc ggcggtaatc cgcgggccgt gcgcgacacg ctcggcctgc tcttgcgcgt
     4861 tcagccgaag actctgtggg agcttctcga gatacgcagg atcctcgaag tcgaggtcgc
     4921 aggcctcgcg gcggagcggg ctgggccgga tgacgtaacg gctatgcggc tccagctcga
     4981 caggatgcgg gactccatcg atgcgcccga gggctacgtc gacgccgacg tcgagtttca
     5041 tgcgttgctg gcgcgggcga cccgcaacga ggtcttactg accatgctcg agcccgtggt
     5101 cgacctgctg cgggcgagcc gccgggtgag cgccgccagg cccggcaatg cgttgagggc
     5161 attaggggag catgaggcga ttctgcgccg ggtggaggca aaagatgcgg gcggagcgcg
     5221 gggaaagatg cgcgcccacc tcgcgaagac ggccgaggac atcgaggccg ccatcggtga
     5281 aggggtgctc atcgccggag cgttctcaga gaaggaggag gtatgaaggc ccacgagatg
     5341 ttgtcagagc tggggttgcc cgggcgcgac ctctacggtc cgcccgtttc ggggaagcga
     5401 ttccccgacg gggcgcagta cagggtcgag atccccagcg tcgaagggcc gcgagtgctg
     5461 gaagctgtta tcgaggaagc cgacaggaga gaagtccaga tccaccgcgt ctctcagggg
     5521 tcggggatca tgctcctcac ggacgccgag atcctggaga tgtgccacat ggcgcgcgag
     5581 tccgccctgg aactctccct cttcgtcggc ccgcgggcct cgtgggagac cggcgcgacc
     5641 gccgtctccg gcgcaggcaa ggtcctcggg gcccagcacc gcggccagga ccagctcgcc
     5701 tacgccatcg aagacgtgct gcgtggtgtc tctttgggac ttcgcggggt gctggtcgcc
     5761 gacgccggac tcctctgggt cctgagggac ctcaaggcga agggagagtt gcccgaagac
     5821 ctcgtcctga aggtctccgt gcagctcgcc gcggccaacg cggcggccgt aaaagcgatg
     5881 gaggacctcg gggcggacac gtacaacgtg ccaacagact tgtcgctggc ccaactcgcc
     5941 gcgatacgcg acaccgtcga cctcccactc gacgtgtacg tcgaggtgcc cgacgacttc
     6001 ggcgggttcg tgaggcacta cgagataccg gacctcgtaa gggtagcttc tcccgtcttc
     6061 gtcaagttcg gcctgaggaa cgcgccgaac atctatccca gcgggacgca cctggaggcg
     6121 accgcggtcg ccctcggaag ggagagggtc aggcgagcca ggatcggaca ggagatgctc
     6181 gcccgctact atccggaggc cgagactacc ccgccgggtg cgaccttccc aggcctgccc
     6241 gtcgacgcca aggtaaagga gaaggcatga aggttaccca cgtagagacg ttcgtggtcg
     6301 gcactgaatg gcgcaacctg accatcatcc gcgcccacac cgacgaggga ttgacgggcc
     6361 tgggagaagc gcggatggtc aaccacacgg acgccctggt ggggtacctg gccgaggcgg
     6421 cccccaggca tctcgtcggg cacgaccctt tcaggataga ggagctggtc aagaagatgg
     6481 gccgcgacga cttctcgcgc gccggcgagg tgatgagcag cggcatcgcg atgttcgaga
     6541 cggcctgctg ggacatcgtc ggcaaggcgc tcggccagcc cgtctacaac ctgctgggcg
     6601 gtccggtgag ggaaagggtc aaggcctacg ccaacggctg gtatagggtg gagcgcacgc
     6661 ccgaggagtt tcacgaagcc gcgaagaagg tcgtagagcg cgggtacagg gctctgaagc
     6721 tggatccctt cggtccagga cattacgagc tggagtacga ggagaagata aaggccgtct
     6781 cgctcgtcga ggccgtccgc gatgccatag gaccggaggt cgagatcctg ctggagatgc
     6841 acggccgctt ctcccccgct accgccatag agatggccag gctgctccag cctttcggac
     6901 tcggctgggt cgaggaaccc gtgcctccgg tcaatctcaa agcgctgaag aaggcttcgg
     6961 atggtattga ggctaccgtg gccacgggcg agcgcatcca ccagcgccac gagtacaggg
     7021 aggtcttcga gcttcaggcc gcggatgtga tacagcccga cattagccac atcggcgggc
     7081 tcctggagac gaagaagctc gcagcgtggg ccgacgctta ctacgtgacc atcgccccgc
     7141 acaacgtggg aggccagatc aacaccgccg cggccctcca cctcgcggcc tgcaccacga
     7201 acttccgcat ccaggagtac ttcaacgact tcgccgaccc ctgggtccgc gagacggccc
     7261 ccggcctctc cgaagtcaga gacggctact tcgagctccc cacgggtcct ggcctcggag
     7321 tcgagctcga cgaggaagtc atcgaagccc accccaagca ggacgtccac ttcaacctct
     7381 tcgccgaggg ctgggagagg cgagagggcg cagagacgaa ccagtgagcg acctctcggg
     7441 caagaaggtc ctggtcaccg gcgccggcat ggggataggc cagggcatcg ccgtggagct
     7501 cgcccgacgc ggggccgagg tcgccgtcca ctacgcccat accgagccgg aagagaccgt
     7561 ctcgcggata aagcaactcg gcgccagggc cgcaacggta cagggcgacc tgagcaaggt
     7621 agaagcctgc gagcgtacgg tggacgctgc ggccgaggcc ctcggcgggc tcgacacgct
     7681 cgtcaacaac gccggcgtca cgaagacgct cgacttcctc gacacggacg aggagaccta
     7741 cgactcggtc ttcgacctca acatgaaggg ctacttcttc ctcgcccgca aggccgtacc
     7801 tctcatggtg gagcggggag gcgggagcat tgtcaacgta acttccgtcc acgggttcgc
     7861 gggcttcccc aaccacacgg cctacgcggc tactaaaggc gctataaacg ccttcacccg
     7921 ccagctcgcc atcgaactca cagaccgcaa ggtccgcgtc aacgccgtcg gtcccggcat
     7981 catagaggtc ccccgctact tcgacatccc gaactatacg accgagttcg gcgataccct
     8041 cgtcccctgg ggccgagtcg gataccccga agacgtcgcc ccagcagtag ccttcctggt
     8101 ctccgacgcc gccgacttcg ttacaggaca ggtcctctac gtggacggcg gcactacggc
     8161 caggatgggc ctctggtggg atcagggaga cggtccagag taacgccagg cgggcgagct
     8221 tgacacggat acacattggt ataacaatgc cggcagggaa gggatgttga aggctagaga
     8281 caggggcgat agatgagtga gcgtggatcc gggctc
//
LOCUS       DATMVF010000241        19518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_443269, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000241 DATMVF010000000
VERSION     DATMVF010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19518
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443269"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VN178_12395"
     CDS             <1..921
                     /locus_tag="VN178_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWS81812.1"
                     /translation="EATLLAKLEYLNPAGSVKDRPAVRMLEIAEKEGLLGPGGTVVEP
                     TSGNTGAGLAMAAAVKGYRCICVMPDKVSKEKQDLLKAMGAEVVVTPTAPPDDPESYY
                     AVAERLAEETPGGFKPAQYENPANPLSHYETTGPEIWRQTEGRVTHFVAGMGTCGTIT
                     GAGRYLKEQSPQVRVVGVDPEGSIFSDPDNVHQYAVEGVGEDFYPGNHDPTVVDEVIQ
                     VDDRESFLMTRRLMSEEGLFVGGSCGMAAVGAVRAAKGVPEGAVVVVLLPDTGRNYVS
                     KVFDDEWVLANSDATQEDLERSYRIFEEGT"
     gene            918..2078
                     /locus_tag="VN178_12400"
     CDS             918..2078
                     /locus_tag="VN178_12400"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HWS81813.1"
                     /translation="MKFEGSGFSTRAIHAGQDPDPATGATIVPIYQTSTYTQEAPGQH
                     KGYEYSRTGNPTRTALEECVAALEGADYGVAFASGLAATTAVMSLLSPGDHVVAGDDL
                     YGGTYRLFDKVLPRSGGLEITYADTTDAASVEEALRPETKLLWIETPTNPMLTLSDIA
                     KLSEMAHERGAFVAVDNTFASPYFQNPLVLGADIVVHSTTKYMGGHSDVVGGAVATSN
                     EEFWEGIKFYQNAAGAVPGAFDSWIVLRGLKTLAVRMRQHEENALAVARFLQDHPEVD
                     EVYYPGLPDHPQHELAKRQMRGFSGMVSFTLKGGAEAAYAAVQKTRVFHFAESLGGVE
                     SLITHPATMTHAAIPREQREARGVTDGLMRLSVGIEDQEDLIADLNGAISSD"
     gene            complement(2117..2911)
                     /locus_tag="VN178_12405"
     CDS             complement(2117..2911)
                     /locus_tag="VN178_12405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWS81814.1"
                     /translation="MTVRVGVQLHPQHASYAQMRDAWLRAEEMGADTIFTWDHFYPLY
                     GDPQGKHFECWTFLGAMAEVTERVEFGALVTCNSYRNPNLLADMARTVDHISGGRLIL
                     GIGSGWFEKDYDEYGYEFGTAGDRLRDLDAAMPVIENRLGKLNPPPLRKIPILIGGGG
                     EKVTLRIVAEHANIWHGFGDPERAARKSGILDDWCEKVGRNKEEIERSISVRPELLGD
                     AGRYVENGITHLIMGFGGPDYDLSPLKDLIAWRDDHRERNGETLAG"
     gene            complement(2970..3707)
                     /locus_tag="VN178_12410"
     CDS             complement(2970..3707)
                     /locus_tag="VN178_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWS81815.1"
                     /translation="MSAALLVAIFAALLAGIVTGLTGFGLALISTPILLFVYEPRTVI
                     VLTTIFSIVINSAVVWDSWQEARRRLALALLIPALFGIVAGAWVLGVINPDYVRLGVG
                     LIVVFSALLLVRDIRLPGAGTRWGTLVAGSASGALSTSTGLAGPPIVLLLASRDLPKH
                     EFRGTSALYFLPMSVVGLAVLAFRGLVESAEIPLGLLLIPAAIAGKALGTALLKHVSE
                     GAFRALTLGLVLLTGTLGVITATLALL"
     gene            complement(3766..4827)
                     /locus_tag="VN178_12415"
     CDS             complement(3766..4827)
                     /locus_tag="VN178_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HWS81816.1"
                     /translation="MVSTTTGSRMMDSTDKEILNRIQREVPLEREPFEAIGRELGIAG
                     DEIIRRIESLKRGRVIRQISAIFDTRVLGYESSLVAARIPAHRLNEGAKAINSHPGVS
                     HNYERNNEFNLWYTVAVPPDSRLGLEGTVDVLHHISGAEKTRILPTLKLFKIGVTLDM
                     NAGATAKKEAPQYGESDRESADRNISEDDKAAIRALQEDVPLTPRPFDLWGRQVGLSH
                     EELLERAYDLRDRKIMRRFSAVLYHRKAGFRANAMGVWRVPDERIDEVGNAFAHYQAV
                     SHCYQRPTYEDWPYSVFSMVHGRSVEECENVLTAMSEETGLTDYTSLYSTREYKKTRV
                     RYFTPEMEAWERLYAGILR"
     gene            complement(4960..5607)
                     /locus_tag="VN178_12420"
     CDS             complement(4960..5607)
                     /locus_tag="VN178_12420"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWS81817.1"
                     /translation="MDSLFDTAEGGESRERRLEELARQVSVCTKCDLAKSRTNTVFAT
                     GDPYSPLMLVGEGPGENEDATGLPFVGRAGKLLDDILAAVDLTRQQVYLTNVVKCRAA
                     VEEGGRMRNRQPKTGEIRACNPYLQGQIEAVKPEIILCLGGPAAKTIIDKDFRITKDR
                     GAWYEIDGIKTMATFHPAYILRQRGDDLNNAKRLVWKDIQNVYAEYQKAMEARES"
     gene            5878..6936
                     /locus_tag="VN178_12425"
     CDS             5878..6936
                     /locus_tag="VN178_12425"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWS81818.1"
                     /translation="MKLRAVLDEIRPYHPPRLEEEISVDRGSKDYVKLTMNELSFGPL
                     PEAREAVVESLARAGRYPDRDADPLREAIAEANSGVGIDNVVVGNGTSEVLVDLMQVV
                     DRPGELVIAWPSFPFYVSAASVVGLRTRKVPLDEHHRADLDAMLDAVSGETRIVILCN
                     PNNPSGTYLPLEEVRSFARALPEDVLLILDEAYYEFVDDPLYVGSHRLALGSENVVST
                     RTFSKVHGLAALRVGYGIASKRLADHVWRAHVPFSVNQAGQAAATVSMRQQGVIARRS
                     RLMKEEREGVQAGFEAAGLEYVPSHTNFVMVRAVPKVFEKTGVLVREGEALGYPVGWS
                     RVTIGSPEENGRLISALS"
     gene            complement(7159..8439)
                     /locus_tag="VN178_12430"
     CDS             complement(7159..8439)
                     /locus_tag="VN178_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HWS81819.1"
                     /translation="MVGFDDGLAQAIRGAVQARREDAVSLLRELVRVPSVTGEEGAVG
                     SVVGRAFSERSLEVDTWEATSEETEPYRDHVGEQSSYEGRPNVAGTRRGMGDGRSILL
                     NAHTDTVDPGDPAAWKGDPLSGDLEGDLLYGRGSCDMKGGLVTYLIALDALSDLGLGL
                     RGDVTVAATVGEENGGLGALSTVLRGYRADAAIITEPTRLALVPAQGGSLIFRLKIPG
                     RSAHAAVRDEGVSALEKFVPVFENLRELELERNGVLSHPLYDALRNKVPINIGVVRAG
                     NWASTVPESLVAEGRVGLIPGEEVDPFKDLVAERIAAVADRDPWLREHPPELEWFGGQ
                     FAPAEVEPDAPICEAIKEAHERVTGEVPAIEGVPYGADMRLFIHFGGMPCVMYGAGDV
                     NVAHAPDEHISITELLTATSTVACLLADWCGVAD"
     gene            8699..9541
                     /locus_tag="VN178_12435"
     CDS             8699..9541
                     /locus_tag="VN178_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWS81820.1"
                     /translation="MTRRSRSHLPAVLVCAFVMAVTLVLPVGCSAGTEESIEGAAPGE
                     TTGPIGIVGEKITVASNIAYPPFESSPKGRPKGFDIELMNEIGKRAGFEVEYENVRFD
                     SILRGLNTDLFDAAISAMTITEDRERQIDFSDPYFNADQALLVESGSGVQSIEDLAEE
                     PVGVQAGSTGQMKAEELLDGEQIGEIAPYRTIGEAFAALEHEKVEGVIYDLSAAHGKI
                     VKRDGKIRFVEPISTGEQYGIAFPKGSPLVDPVNKALAEIKDDGTYEKLYKKWIGMPP
                     EDIP"
     gene            9811..11385
                     /gene="murJ"
                     /locus_tag="VN178_12440"
     CDS             9811..11385
                     /gene="murJ"
                     /locus_tag="VN178_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWS81821.1"
                     /translation="MTAILRSVLSISVATVLSRATGYARWMAQAAALGAGYVADAFTA
                     AILLPGLIYELFMGGILYSIFIPVLVDRITKVGEEDARRFTNALFTLVLPLMVVLSVA
                     GIVFARPLVLLVTDWQSGLPPAEAGRTLDLAVLFFRVFSLQMLFYGISTISTGVLQAH
                     RRFFLPTFAPVLNNLMIIASFVAYVLLVDADQTLALYVLAVGVTAGLAVMALALVPTM
                     LSLGYRPRPQFGHPALLPTARLAGPMVVLVAASVAFQLFGAYLATGFEALSELNYAFT
                     IFSLPYGVFVVAIATALMPELSENHSRRDADGYRETFSFGLRTMIFVVVPSAVGMISL
                     SEPIVGLLYERGSFDATDTLTVSGLLVVYAAGLLGYSVYFFLVRAFYSRQNTKTPALL
                     NVAIFVLYAGLAYGLSRVWGVTGVVLALSIAYAVLAALGLAATRQEIGRIEGRRLVRS
                     LLKILAAGTVMYVVASAGTLLLGTGSDFTGRLFVLVTVGGASLAVYIGAAYLLRAEEL
                     ESALSLLRRRAGKAAG"
     gene            complement(11531..13000)
                     /locus_tag="VN178_12445"
     CDS             complement(11531..13000)
                     /locus_tag="VN178_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWS81822.1"
                     /translation="MNLFPRRKPWYKRRRRRLPAAILVALVLLAGAAYVVTSTRDGTA
                     NLSGSSGRDTPSQTREEARSEGVPTKGSDQGEPPPEETAPDPEQDSPEAAAYVALAPE
                     LPGTTPQSIKGVYRSKIDESWASVRVQPENEGKPFVVFTHKDGESWRAEKSVRADEPN
                     YAKNDVVPLSGVPKDLIEYLYTENVFAAEVPEPKVEKLNLEDLPDAGPAEFSSTEPVM
                     DGVPESDRERVQKVLEGARQKIEDYDGVAGLYVWDLEGDSGYGIRPDEPFFAASTIKI
                     PIMVAVYRKVDEGELEFSQEVEILEEDWAAGAGWLQWEKAGTRQTVGDLLVLMMTQSD
                     NVASNALVRTVGGADHVNEVARTMGAEDTVLYQKVSSERGVVPALDNRSTPRDMATMM
                     QQIAEGKAASEKSCGYMIDLMHENKLDWWLDEGLPPGVDAANKAGWLYRVYDEVGIVE
                     EDGRHYAMAILSKHGGADVYQGQDLIKNLSSAVWEAQEK"
     gene            complement(13007..14440)
                     /locus_tag="VN178_12450"
     CDS             complement(13007..14440)
                     /locus_tag="VN178_12450"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HWS81823.1"
                     /translation="MSKTGGPGTARRYNHDTPRAVKLKRRRTTFARRRKTAGVALIAC
                     FLLIGTLIVYSLTGGDDEIGRGVSIGSVEVGGMTRDEAREAVQDDASATFEQISFGTS
                     KEVFSVNGDDLGIKVDATSAVDEAYSVGRRGNVFQHLSDASRSYLGGVRVDLEAGYDR
                     DMARSILEERAGEFNEEPKDASFSVTESGKVEVEEAEKGRVMDQEETLANLEGALKNM
                     SGRVAIAEGPPPEPEVSTAEVQSHRPKEVIGEYQTDFLWDSNPNRKYNMKLAARSVNN
                     TVLKPGEVFSFNDLTRSLDYEEAKTFSNGGVGIANGGGLCQVSSTLYMAANYAGLQIL
                     ERHPHYAVLPYIKPGFDATVWFGENGWGALDMRFKNNTDGYIVVREWVDEKGLLNAQI
                     LGRPTGKKVEMSTQKIYEDPVKGIKWTTYKKVTEDGEVIRDGFLYTYRYSFNPPPPEN
                     APHFKTTAPRVAGWSDPTNTTGWASPH"
     gene            14782..16029
                     /locus_tag="VN178_12455"
     CDS             14782..16029
                     /locus_tag="VN178_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81824.1"
                     /translation="MPASLCEERRIGVLPMKAAVEPVEGPGLEDKIGQLRILAFPDFP
                     EVHEVDYYSALYRWYEDHPLAGELYRWVSVTQEGEVVGHLTAFPQFYRIDGRRVVAHT
                     PGDYMVLPQHGFQAIMLMRSFFRQVENCVVCDMVPAVIGIETRLGAEVVGDLQYAAKL
                     MNVSRLPMPSVPAPLRRLLKLPEQFAPARGFTDRPGAEPHEVEEHVAPPAPRPRAPIP
                     RPLQGLLNGGLRAVDDALGSSLARDLAVEVLDGFDDSFDDLFDRVAAVVPCTAEKDAA
                     FLSWRYGPGSPQSPVTVLGVRSSGRLLGYAVLKAAFHGQDGYILDLTALPGRQDVVLA
                     LLRETVSSFGRMGVQIIRYRFVGSPTSPRSADLLRLGFFSRRGRRHKLLVKFTDTNLR
                     GTTGDLVNWSLSFGDGEAGFWLR"
     gene            16042..16293
                     /locus_tag="VN178_12460"
     CDS             16042..16293
                     /locus_tag="VN178_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWS81825.1"
                     /translation="MISGNGDARAAIRGWLQDNVTGGRDVPNDEPLIDNGVMTSLQTV
                     ELVVFLEDTFGIVVEDEEFDEENFGSVEAIARLVESKAA"
     gene            16290..17879
                     /locus_tag="VN178_12465"
     CDS             16290..17879
                     /locus_tag="VN178_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWS81826.1"
                     /translation="MRPRPEFTLHDLLYNSAARDPQRAAVVDGDTKYTYEDLQRQSGS
                     LGAALAEAGVRRGDRVGVYLEKSWEAIVAMLAASRIGAAYVNVNPLFKAPQVEYLAGD
                     CDIRVMIGDTSRLEELQPKSVQTAFYKGAKPEGPAARTYQDVAEALEGEGLTEDRNVS
                     ESDLGTILYTSGSTGMPKGVSTSQRNVVAGAQIVSTYLENKPEDRILSALPLNFDAGM
                     SQFTTSLRAGATLYLLRSRLPGDLLKALRRHEITGVTGVPPLWALLILGARSIREKPL
                     THLRYLANTGGRIPQANLDELRDLLEPAGTRIFLMYGLTEAFRSTYLAPEEIHRSSPE
                     QGNCIGKAIPNTEIMVITKDGEEAAPGEPGELVHRGPTVAMGYWGNEEATRRAYRPNP
                     LAPPELLDIERVVYSGDTVERDAEGYLYFLGREDAMIKNQGYRLSPEEVENLLLSSGL
                     VHEACAFGVENPAVGQDVIAVISLRKGSEEGAVDRVREYAINNGPPYMVPKEILVKDE
                     LPKTGSGKIDRKGISSAYSNG"
     gene            17860..19206
                     /locus_tag="VN178_12470"
     CDS             17860..19206
                     /locus_tag="VN178_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HWS81827.1"
                     /translation="MLTQTDKANDLAGRFGGESGGEFAPAGKPISGLAREFGTPFYLY
                     HGEMISERVGRVREALGTEVSYSVKANPSLGVCQLIASEREAGAEVASSGELAVALAA
                     GFEPQDIVFAGPGKTDDELRRAVTEGIFAVNVESVHEIDRLARLAREAGKTIGVGLRI
                     NPEAQLMGSGMRMGGTVGQFGMDQAELGEGVRKTLGRAELVLRGVHVYTATQVFEVGP
                     LLEHCRNILEISLEAADLAGRPLEMIDFGGGFGVPYFEKMSEFDLDGFAEGFRDLLSS
                     FRSDPRLRGCRYLFELGRYLVADAGVYVTRVVDVKETRGKTFAVTDGGMNHHLTATGN
                     MGQVFRKAYPLLNLTRMGGVPEEGIAVAGPCCTPLDMFGNNITLAAPEEGDLIGVFYS
                     GAYGYSASNLGFLSHPAPAEVLLWRGGAHLLRPGGSPEDVLSGQRPLSKPESGVPG"
BASE COUNT         3312 a         6321 c         6263 g         3622 t
ORIGIN      
        1 gaggccaccc tcctcgcgaa gctcgaatac ctgaatcccg ccggctcggt aaaggacagg
       61 cctgctgtca ggatgctcga gatcgccgag aaggaggggc tactcgggcc cggcgggacc
      121 gtcgtcgagc cgacgagcgg gaacacggga gccggcctgg ccatggccgc agcggtcaaa
      181 ggctacaggt gcatctgcgt gatgccggat aaggtgagca aagagaaaca ggacctgctg
      241 aaggctatgg gcgccgaggt ggtcgtcacg ccgactgctc cgcccgatga tcccgagagt
      301 tactacgccg tcgccgagag gctggcggag gagacccccg gcggcttcaa gcctgcccag
      361 tacgagaacc cggccaatcc tctctcccac tacgagacga cgggacccga gatctggcgc
      421 cagacggaag gacgtgtaac acatttcgtc gccgggatgg gcacctgcgg aaccatcacg
      481 ggagctggcc gatatctcaa ggagcagagc ccccaggtca gggtcgtcgg cgtcgacccc
      541 gaaggatcta tcttctccga ccccgacaac gtacaccagt acgccgtcga gggcgtcgga
      601 gaggacttct accccggtaa ccacgacccg accgtggtgg acgaggtgat ccaggtagac
      661 gaccgcgaat cgtttctcat gacccgccgc ctgatgtcag aagagggcct cttcgtcggg
      721 ggttcgtgtg ggatggccgc cgttggggcg gtccgcgcgg cgaagggagt gcccgagggc
      781 gccgtggtcg tcgttctgct gccggacacc gggcgcaact acgtctccaa ggtcttcgac
      841 gacgaatggg tgctcgccaa ctcggacgct acgcaggagg acctcgaaag gtcctaccgc
      901 atcttcgagg agggaacatg aagttcgagg gcagcgggtt ctccacgcgc gccatacacg
      961 ctgggcagga ccccgacccg gcgacggggg cgacgatagt ccccatctac cagacctcca
     1021 cctacactca ggaggcgccg ggccagcaca agggctacga atacagcagg acgggcaacc
     1081 cgactcgcac agccctggaa gagtgcgtgg cggcgctcga aggggccgac tacggcgtcg
     1141 ccttcgcctc cgggctcgcc gcgacgaccg cggtgatgag cctcctctcg ccgggggacc
     1201 acgtcgtcgc cggggacgac ctctacggcg gcacctatcg cctcttcgac aaggtcctcc
     1261 cgcgctccgg cgggctcgag atcacctacg cggataccac ggacgccgcc tcggtcgagg
     1321 aggcgctgag gccagagacg aaactgctct ggatcgagac ccctaccaac ccgatgctca
     1381 ccctctccga catagcgaag ctctcggaga tggcgcacga gcggggcgcc ttcgtcgccg
     1441 tggacaacac cttcgcctcg ccttacttcc agaacccgtt ggtactcggg gctgacatcg
     1501 tcgtccactc gacgaccaag tacatgggcg gccactcgga cgtggtgggc ggggccgtcg
     1561 cgacctccaa cgaggagttc tgggagggga tcaagttcta ccagaacgcc gccggcgccg
     1621 tacccggcgc gttcgactcc tggatcgtac tccgcggcct caagaccctc gccgtccgaa
     1681 tgcgccagca cgaggagaac gcactggcgg tggcgcggtt cttgcaggac catcccgagg
     1741 tcgacgaggt gtactatccc ggcctgcccg accatcccca gcacgagctg gcgaaaaggc
     1801 agatgagggg gttttcgggg atggtctcgt tcaccctcaa aggcggcgcg gaggccgcct
     1861 acgcggcggt gcagaagaca cgagtcttcc attttgccga gagtctgggc ggggtggagt
     1921 ccctgatcac ccatcctgcc accatgaccc acgccgcaat cccgcgcgaa caacgcgagg
     1981 cgaggggggt tacagacggc ctcatgcgcc tctctgtcgg catcgaggac caagaggacc
     2041 tcatcgccga tctaaatggg gcgataagct ccgattagtc tggctccgga gggttcggcg
     2101 gccgatctcg ggccgatcag cccgcgaggg tctctccgtt gcgctccctg tggtcgtcgc
     2161 gccaggcgat gaggtccttc agggggctca ggtcgtagtc ggggccgccg aagcccatta
     2221 tgaggtgggt gatgccgttc tccacgtagc ggccggcgtc cccgaggagt tccggcctga
     2281 ccgagatgga gcgctctatc tcttccttgt tgcgccctac cttctcgcac caatcgtcca
     2341 ggatgccgct ctttcgggct gcccgctccg ggtccccgaa accgtgccag atgttggcgt
     2401 gttcggcgac gatgcgcagg gtgaccttct cgcctccacc gccgatgagg atagggatct
     2461 tgcgcagggg cggcgggttc agcttcccga gccggttctc tatgacgggc atcgcggcgt
     2521 cgaggtcccg cagcctatca cccgccgtcc cgaactcgta gccgtactcg tcgtagtcct
     2581 tctcgaacca gccagagccg atcccgagta tgaggcgtcc gccggagatg tggtcgaccg
     2641 tacgtgccat gtcggcgagc aggttcggat tgcggtaaga attgcacgtg acgagcgccc
     2701 cgaactctac cctctccgtg acctcggcca tcgccccgag gaacgtccag cactcgaagt
     2761 gcttgccttg ggggtcgccg tagagcgggt agaagtggtc ccaggtgaaa atagtgtcgg
     2821 cgcccatctc ctctgccctg agccacgcgt cgcgcatctg cgcgtaactg gcgtgctgcg
     2881 gatggagctg caccccaacc cttaccgtca tccttcgcct cctcatctgg ccaaccggga
     2941 gattatgcca cgtggaggac gcgaccgggc tacagcagcg ccagggtggc ggtgatgaca
     3001 ccgagggtgc cggtcaggag aacgagccct agagttagtg cccggaacgc cccttcggag
     3061 acatgcttga ggagcgccgt gccgagtgcc ttcccggcta tggcggcggg gatgagcaag
     3121 agccccaggg ggatttccgc ggactcaacc aggcctcgga atgccaggac cgcgagtccg
     3181 acgacgctca tgggcaagaa gtacagcgcg ctcgtccccc tgaactcgtg cttcgggagg
     3241 tcgcgcgagg ccaggagcaa cactatgggg gggccggcga gcccggtcga ggtcgagagc
     3301 gccccgctgg ccgaaccggc gacgagggtc ccccaccgcg tgccggcccc gggcagacgg
     3361 atgtccctta ccaggagcag ggccgagaag acgacgatca gaccgacccc gaggcgcacg
     3421 tagtcggggt ttatcacgcc aagcacccag gcgccggcca cgatgccgaa gagggcgggg
     3481 atcaggagcg caagcgcgag ccggcgcctc gcctcctgcc aggagtccca cactacggcg
     3541 ctgtttatga cgatcgagaa gatggtcgtc agcacgatga ccgtacgggg ctcgtagacg
     3601 aacagaagta tgggcgtgct gatcagggcc agcccgaaac ccgtaagccc ggtgacgatc
     3661 ccggcgagca acgccgcaaa gatcgccacg agcaacgccg cgctcaacaa ggaaggagtc
     3721 ctcccccgaa acctgaagcc tgtagcctga tgcctgaaac ctgattcagc gcaggatgcc
     3781 ggcgtagagg cgctcccagg cctccatctc cggcgtgaag tagcgcaccc gagtcttctt
     3841 gtactcgcgg gttgagtaga gggaggtgta gtccgtgagt cccgtctcct cggacatcgc
     3901 cgttagtacg ttctcacatt cttctacgct gcggccgtgg accatggaga agacggagta
     3961 cggccagtct tcgtaggtcg gccgctggta gcagtgggag acggcctggt agtgggcgaa
     4021 ggcgttgccc acctcgtcga tgcgctcgtc gggcaccctc cagaccccca tcgcgttggc
     4081 cctgaagccg gccttgcggt ggtagagaac cgcagagaag cggcgcatga tcttgcggtc
     4141 gcgcaggtcg taggccctct ccaggagctc ctcgtgggag aggcctacct gccttcccca
     4201 caggtcgaaa ggacgcggcg taagcgggac gtcctcctgg agggccctga tggccgcctt
     4261 gtcgtcctcg gagatgttcc tgtcggcgct ctccctgtct gactcgccgt attggggggc
     4321 ctccttcttc gccgtagcgc cggcattcat atccagggtc accccgatct tgaagagctt
     4381 cagggtgggc aggatgcggg tcttctctgc gccgcttatg tggtgcagca cgtccacggt
     4441 gccctccagc cccaggcggg agtcgggcgg gacggcgacg gtgtaccaga ggttgaactc
     4501 gttgttgcgc tcgtagttgt gggagacgcc ggggtgggag tttatggcct tggcgccctc
     4561 gtttaacctg tgggccggga tcctggcggc gaccaggctc gactcgtagc cgaggacgcg
     4621 cgtgtcgaag atggcgctga tctggcgtat cacccgcccc ctcttgagcg actctatcct
     4681 ccggatgatc tcgtcgcccg cgatccccag ctcgcgcccg atggcctcga aaggctcccg
     4741 ctccagcggg acctcgcgct ggatccggtt gagtatctct ttgtcggtgc tgtccatcat
     4801 gcgactgccg gtagttgtgc tgaccacgct ttacgagccc ttctcgacga tttctttcta
     4861 cagctttgca ctatatcagg cgcagccaaa acccggtatg agaatgttta cgttgttcaa
     4921 tgcgatttgc tgaggtcaat acctcagcaa atcgcgccgt caggactctc gcgcttccat
     4981 cgccttctgg tattcggcgt agacgttctg gatgtctttc cagaccaggc gcttggcgtt
     5041 gttcagatcg tcgccacgct gcctgaggat gtaggcggga tggaaggtcg ccatcgtctt
     5101 gatgccgtcg atctcgtacc aggcgccacg gtccttggtg atcctgaagt ccttgtctat
     5161 gatcgtcttc gcggcgggac cgccgaggca gaggatgatc tccggcttga cggcctcgat
     5221 ctggccctgc aaataagggt tgcaggccct tatctccccg gtcttcggtt ggcggttgcg
     5281 catcctgcca ccctcttcga cggcggccct gcacttcacc acgttcgtca ggtagacctg
     5341 ctgcctggtc aagtccacgg ccgccagtat gtcgtcgagg agcttgcccg ccctcccgac
     5401 gaaaggcagt cccgtggcgt cttcgttctc gccagggccc tcgccgacga gcatcagggg
     5461 cgagtaggga tctcctgtcg cgaagaccgt gttggtgcgg ctcttcgcca ggtcacactt
     5521 ggtgcagacg gagacctggc gggcgagttc ctcgagccgt ctctcgcggc tctcgccgcc
     5581 ctcggcagta tcgaaaagac tatccaaagc tcctccgcag gctgtcctgg tgactttccc
     5641 gccaattgta ccccggcgac gccggctcct aacgcgtgac aagcaccggg cgcgtggtac
     5701 agcatctgtt cacccgcggc actaatgccg ggtgttacgg attgtatcga ccgcggatag
     5761 cccgatcaaa ccgccggccg cgcgggggcc gcgttgcggg ggccgcgttg cgggggccgc
     5821 tcgggtgcac tatcttctct tgcaaagtcg tttgtctctc ggagaggaag acttatcttg
     5881 aagctaaggg ctgtgctgga cgagatacgt ccctaccacc cgccgcggct ggaggaggag
     5941 atctcggtcg accgcggctc gaaggattac gtcaagttga cgatgaacga gctgtccttc
     6001 ggcccgctcc ccgaggcgag ggaggcggtc gtcgaatctc tcgcaagggc cgggcgctac
     6061 cccgaccgcg acgccgaccc gctgcgtgag gcgatagccg aggcgaactc tggtgtcggc
     6121 atcgataacg tcgtcgtcgg gaacggcacg agcgaggtgc tggtcgacct tatgcaggtg
     6181 gtggaccgcc ccggtgagct agtcatcgcc tggccctcgt tccccttcta cgtctcggct
     6241 gccagcgtgg tcggactgcg gacgcgcaag gtcccgctcg acgagcacca cagggccgac
     6301 ctggacgcca tgctcgacgc cgtctccggc gagacgcgca tcgtgatcct ctgcaacccg
     6361 aacaacccgt cgggcacgta cctgccgcta gaagaggtgc gctcgttcgc gcgggcgttg
     6421 ccggaggacg tgctcctcat cctggatgag gcctactacg agttcgtcga cgatcccctg
     6481 tacgtcggct cgcaccgact ggccctcggc tccgagaacg tcgtctccac caggaccttc
     6541 tccaaggtgc acggtctcgc ggccctcagg gtcggctacg gcatcgcctc aaagcgactg
     6601 gcggaccacg tctggcgcgc ccacgtgccg ttcagcgtga accaggcggg gcaggcggcc
     6661 gccacggtct cgatgcgcca gcagggagtc atcgcccgac gctcgcgcct catgaaggaa
     6721 gagcgcgaag gcgtccaggc gggtttcgag gcggcgggcc tggagtacgt gccctcgcac
     6781 acgaacttcg tcatggtccg cgccgtcccc aaggtcttcg agaagaccgg cgtgctggtg
     6841 cgcgagggcg aggccctagg gtatcccgtc ggatggagcc gggtcacgat cggcagcccc
     6901 gaggagaacg ggcgtctgat ctccgcgctc tcctagcccg gggatcttac agcgggttgc
     6961 aggaggatgg acctcctgca acccgcgccg tcagctctca gctattagcc ttcagctctt
     7021 tgttcttggc tgaaagctga tagcttatcg ctgatagctc gtcgtgcata agggttcaac
     7081 ccttatgcac gacgctgtca gccggttcgg caggcgtttc tcgagccggg ataccgcggt
     7141 gtaaagctcc tgactcttct agtcggccac gccgcaccag tcggccagga ggcaggcgac
     7201 cgtggaggtc gcggtcagaa gctcggtgat gcttatgtgc tcgtcggggg cgtgcgccac
     7261 gttcacgtcc cctgcgccgt acatcacgca gggcatgcct ccgaagtgga taaagaggcg
     7321 catgtccgcg ccgtagggga cgccttctat ggctggcacc tcacctgtca cccgctcgtg
     7381 ggcctccttt atggcctcgc agatcggggc gtccggctcg acttcggcgg gggcgaactg
     7441 gccgccgaac cactccagct ccggaggatg ctcgcgcagc caggggtccc gatctgctac
     7501 ggcggcgata cgttcggcaa ccaggtcttt gaagggatct acctcctcgc cggggataag
     7561 gccgacgcgg ccctccgcaa cgagggactc ggggacggtc gaggcccagt taccggcccg
     7621 tacgacgcct atgttgatgg gcactttgtt acgcagggcg tcgtagagtg ggtggctgag
     7681 cacgccgttg cgctccagct ccagctcgcg caggttctcg aagacgggga cgaacttctc
     7741 cagcgccgag acgccctcgt cgcggacggc ggcgtgggcc gagcggcctg ggatcttcag
     7801 ccggaagatg agcgaaccgc cctgggcagg caccagggca agacgggttg gctcggtgat
     7861 gatcgcggcg tcggcgcggt agccgcgcaa aacggtggag agggcgccaa gtcccccgtt
     7921 ctcctcccct accgtcgccg ccacggtgac gtcgccgcgc agtccgagac cgaggtcaga
     7981 aagggcgtcg agcgcaatga ggtaggtgac gagcccgccc ttcatatcgc aggagccgcg
     8041 tccgtagagc aggtctccct ccaggtcgcc cgagagtgga tcccctttcc acgcggccgg
     8101 gtctcccgga tccacggtgt cggtgtgggc attgagcagg atcgagcgtc cgtctcccat
     8161 ccctctcctc gtgccggcga cgttcggtcg accctcgtaa gagctctgct ctccgacgtg
     8221 atctcggtac ggttcggtct cctcgctcgt ggcctcccac gtgtccacct cgaggctacg
     8281 ctccgagaag gcgcgcccga cgaccgaccc cacggcaccc tcttcgcccg taacagaagg
     8341 gacgcgcacg agctcccgta gcaggctcac tgcgtcctcg cgcctggcct gtacggcgcc
     8401 gcgtatggcc tgtgcgagcc cgtcgtcgaa ccctaccatc agccgcgtcc tccctctctg
     8461 gtgccggtaa ggcggcaccg tagcatggcc cccccttcca ggcaacaccc ggcttgcttg
     8521 accagatgcc gatgcgtaat tagtattgtc gttctggagc ggggcttcga gagcgagtac
     8581 cccgtgcaac gagatgtcgt acctagccaa ctcgccggcg gccgggtccg gccgcagtca
     8641 ccctttccac cgcggccggg tcagactaga gggcagacaa caggaggata agttcaaggt
     8701 gacgagacgc tctaggagcc accttccggc ggtgctcgtc tgcgcctttg tcatggccgt
     8761 tacgctggtc ctcccggtcg gctgcagcgc gggaaccgaa gagtccatcg agggcgccgc
     8821 tcccggcgaa accacgggcc cgatagggat agtgggcgag aagatcaccg tcgcctccaa
     8881 catcgcctat ccgcctttcg aatcctcacc caaaggcaga ccgaaaggat tcgatataga
     8941 acttatgaat gagatcggga agagggccgg cttcgaggtc gaatacgaga acgtccgctt
     9001 cgacagtatc ctgcggggtc tgaacaccga tctcttcgac gctgctatct cggcgatgac
     9061 catcaccgaa gatcgcgagc ggcagataga cttctccgat ccctacttca acgccgacca
     9121 ggcgctcctg gtggagagcg gttcaggagt ccagtctatc gaggacctcg ccgaagagcc
     9181 cgtcggcgtc caggccggct cgacgggcca gatgaaggcc gaagagctcc tggacggcga
     9241 gcagatcggg gagatcgcac cttacaggac cataggggaa gccttcgcgg cgctcgagca
     9301 cgagaaggtc gagggggtga tctacgacct ctctgccgcg cacgggaaga tcgtaaagcg
     9361 cgacgggaag atcaggttcg tcgaacccat ctcgacgggc gagcagtacg ggatcgcgtt
     9421 ccccaaaggc agtcctctcg tcgatcccgt caacaaggcc ctggccgaga tcaaggacga
     9481 cggcacctat gagaagctct acaagaagtg gatcgggatg cctcccgagg acataccgta
     9541 gagagcttgt cagcacgctg ccttcggcag cttgtcagcc cggcgccgtt ggcgccttgt
     9601 cagcacgctt cgggcctata aggccctcgc ttgtcaggtt ctcagcttct tccgctccgg
     9661 cttcaccctg ccgcgcacga gataccgctg ctgaggtggt cgggttctac gctcgtggcg
     9721 aggccacgaa acgacttgct gaagtgctga caaggcggcg cagccgccgg gctgacttgc
     9781 tgacaagcgc ccgacagggc gcgtgctgat atgactgcca ttctgaggtc cgttctctcc
     9841 atctccgtgg cgacggtcct ctccagggcg acggggtacg cgaggtggat ggcccaggcc
     9901 gcggccctcg gcgccgggta cgtggccgat gcgttcacgg ccgccatcct tctgccgggc
     9961 ctgatctacg agcttttcat gggcggcata ctgtattcga tctttatccc cgtcctggta
    10021 gaccgcatta cgaaggtggg agaggaagac gccaggcggt ttacgaacgc cctcttcacc
    10081 ctggtactgc cgctgatggt ggtgctttcg gtcgcgggca tcgtgttcgc caggccgctg
    10141 gtattgctcg taaccgactg gcaatcgggg ctccccccag ccgaggccgg gaggacgctg
    10201 gatctcgcgg tgctgttctt ccgggtcttc agtttgcaga tgctcttcta cggcataagc
    10261 accatcagca cgggcgtctt gcaggcccac aggcgcttct tcttgcccac cttcgccccc
    10321 gtgctgaaca acctgatgat catagcctcg ttcgtcgcct acgtcctgct cgtcgacgcc
    10381 gaccagaccc tggccctcta cgtgctcgcg gtgggggtca cggcggggct cgcggtcatg
    10441 gccctggcgc tggtgccgac gatgctgagc ctcggataca ggcctcgccc gcagtttggc
    10501 caccccgctc tgctccccac ggccaggctc gcagggccga tggtcgtact ggtggccgcc
    10561 tccgtagcct tccagctctt cggcgcctac ctggcaacgg ggttcgaggc cctctctgaa
    10621 ctcaactacg ccttcactat cttctcgctc ccctacggcg tcttcgtggt cgccatcgcc
    10681 accgcgctca tgcccgagct ctccgagaat cactcccgca gggacgctga cggttaccgc
    10741 gagacgttct ccttcgggct gaggacgatg atcttcgtcg tcgtaccgtc ggcggtgggc
    10801 atgatctcgc tctccgagcc catcgtcggg ttgctctacg agcgcggaag tttcgatgca
    10861 acggacacct tgacggtctc cgggttgctc gtggtctacg cggcgggact gctcggctat
    10921 tcggtgtact tcttcctcgt gcgggccttc tattcgcggc agaacaccaa gaccccggcc
    10981 ctgttgaacg tcgccatctt cgtgctctac gcgggtctcg cctacggcct ctcccgcgtc
    11041 tggggcgtga cgggcgtggt gctcgccctt tctatcgcct acgccgtgct cgccgctctg
    11101 ggcctcgcgg cgacgcgcca ggagataggc cggatagagg gccggcgcct cgttcgctct
    11161 ctgctcaaga tcctcgcggc agggacggtc atgtacgtcg tcgccagtgc gggaacccta
    11221 ctcctcggga cgggctcgga ctttacaggg cgcctcttcg tcctcgttac ggtgggcggc
    11281 gcctcgctgg ccgtctacat cggggcggcg taccttctga gggcggagga gctagagtcc
    11341 gccctctcct tactcaggcg gcgagccggg aaggccgcgg gataaggcat caggtttcgg
    11401 gtcacaggct tcagaactgc tccctggcac ctgaccactt tcgaatgcgc agacctgaac
    11461 gtggcggctg acaacgggtt tgaatagcgg aaggcagtct ctgtgtcttc ccctacctga
    11521 tgcctgtagc ctatttctct tgcgcctccc aaacggcgct cgaaaggttc ttgatcaggt
    11581 cctgcccctg gtacacgtcg gccccgccgt gcttgctgag gatcgccatg gcgtagtggc
    11641 ggccgtcttc ctcgacgatg cctacctcgt catagaccct gtagagccag cctgccttgt
    11701 tcgccgcatc cacgcctggg ggaaggcctt cgtcgagcca ccagtcgagc ttgttctcgt
    11761 gcatgaggtc gatcatgtac ccgcaactct tctcgctcgc ggccttgcct tcggctatct
    11821 gttgcatcat ggtagccata tcgcggggcg tgctcctgtt gtcgagggca ggaacgacgc
    11881 ccctctcact actcaccttc tggtagagca ccgtatcctc ggcccccata gtacgtgcga
    11941 cctcgttgac gtggtcggcc ccacccacgg tgcgcaccag ggcgttggag gcgacgttgt
    12001 ctgactgggt catcatcagg accaacaggt cccccacggt ctgcctggtc cccgccttct
    12061 cccactgcag ccatcccgcc cctgcggccc agtcttcttc caggatctcg acctcctgcg
    12121 agaactcgag ctcaccctcg tccaccttgc ggtagacggc caccattatg ggtatcttta
    12181 tggtggaggc cgcgaagaac ggctcgtcgg gccttatccc atagcccgag tccccttcga
    12241 ggtcccacac gtagagaccc gcaacaccgt cgtagtcctc gatcttttgc ctcgcccctt
    12301 cgagaacttt ctgcacgcgc tcgcggtcgg actcgggcac accatccata actggctccg
    12361 tagatgagaa ctccgcaggt cccgcgtcag gcaggtcttc gaggttcagt ttctcgactt
    12421 tcggctcggg gacttcggcc gcgaacacgt tctcggtgta gaggtactcg atgaggtcct
    12481 tcggcacgcc ggagagcgga accacgtcgt tcttcgcgta gttcggctcg tcggcgcgca
    12541 cggacttttc ggccctccac gactcgccat ccttgtgggt aaagacgacg aaaggctttc
    12601 cctcattctc gggctggacg cgtacggagg cccacgactc gtctatcttg ctccggtaca
    12661 cgccctttat gctctggggg gtcgtacccg gcagctcggg ggcgagggcc acgtaagccg
    12721 cggcctcggg cgaatcctgc tcggggtctg gcgcggtctc ctcaggcgga ggctccccct
    12781 ggtccgaccc cttggtcgga accccttcgg atctagcctc ctcgcgagtc tgggaggggg
    12841 tgtcccttcc agaagaaccg gagaggttgg ccgtcccatc ccgagtactg gttacgacgt
    12901 atgcggcccc ggccaggagc accagggcca ccaggatggc ggccggcagc cggcgacggc
    12961 gacgcttgta ccagggctta cggcgtggaa acagattcaa ggcgcgctag tgggggcttg
    13021 cccaaccggt ggtgttcgtt ggatcggacc acccggcgac cctgggggcg gtggtcttga
    13081 agtgcggcgc gttctccggg ggtggcgggt tgaagctgta gcggtacgtg tagaggaagc
    13141 cgtcgcggat cacttcgccg tcttcggtca ccttcttgta ggtggtccac ttgatccctt
    13201 tgacgggatc ctcgtagatc ttctgcgtgc tcatctctac cttcttgccc gtgggccggc
    13261 cgaggatctg ggcgttgagc aagccctttt cgtcgaccca ctcccggact acgatgtagc
    13321 cgtcggtgtt gttcttgaac cgcatatcca gggcgcccca gccgttctcc ccgaaccata
    13381 ccgtcgcgtc gaagccgggc tttatgtagg ggagcacggc gtagtgcggg tgacgctcca
    13441 agatctggag cccggcgtag ttggccgcca tgtacagcgt cgaggacacc tggcagagcc
    13501 caccaccgtt ggcgatgccg acgccgccgt tggagaaggt cttcgcctcc tcgtagtcga
    13561 gtgacctggt caggtcgttg aacgagaaca cctcgccggg ttttaggacg gtgttgttca
    13621 cggatctggc ggccagcttc atgttgtact tgcggttcgg gttcgagtcc cacaggaagt
    13681 cggtctggta ctcccctata acctccttcg gcctgtgact ctggacctcg gccgtagaga
    13741 cctccggctc tggagggggt ccttcggcta tggccacccg accgctcatg ttcttcagcg
    13801 caccttcgag gttcgccagg gtctcctcct gatccatgac gcgacccttt tcggcctcct
    13861 cgacctctac cttgccgctt tcggtgacgc tgaaggaggc gtccttgggc tcctcgttga
    13921 actcgccggc ccgctcttcc agaatgctcc tggccatgtc tctgtcgtac ccggcttcga
    13981 ggtctaccct aacccctcca aggtacgagc gtgaggcgtc ggagaggtgc tggaatacgt
    14041 tcccccggcg tccgacggag taggcttcgt cgaccgcaga ggtcgcgtcc acctttatgc
    14101 cgaggtcgtc tccgtttacg gagaacacct ctttagaggt gccgaaggag atctgctcga
    14161 atgtagccga ggcgtcatcc tgcacggcct cgcgggcctc gtctctggtc atcccgccaa
    14221 cctcgacgga gccgatgctg acgcctcggc ctatctcgtc gtctcccccg gtcagggagt
    14281 agacgataag agtcccgatc agcaggaaac acgcgatcag ggccacgccc gcggtcttgc
    14341 gcctgcgcgc gaaggtcgtc ctcctgcgct tgagcttcac cgcccgtggc gtatcgtggt
    14401 tgtaccgccg ggcggtgccc ggtccacccg tcttgctcat ctctttcaag cagtcgcccc
    14461 tcctgcgata ccaattagcg ccaccggggt cgcggtggca cacaggcgcc aattatctgg
    14521 gatatcgttc gaagcttcaa cctccctgga gtgaacgccg tgaaaacact cctgccttat
    14581 tggaacacta ccacagaggg ccgctaccgt tcacgagaga tgccaggcca cgaggtgcgt
    14641 ggcttggctt ttgcgaacgg ctccattaca ctttgccctt tggatctgca ccgtgaaacc
    14701 ctacagagtg gaagggccct tgcgtcaccg atcagaaggt aagagagcaa gccatggagc
    14761 acgtatgccg ggaagatcgg cttgccggct tccctttgtg aggagcgccg gatcggggtt
    14821 ctgccgatga aggcggccgt ggagccggtc gaggggccgg ggctggaaga caagatcggg
    14881 cagctgcgca ttctcgcgtt cccggatttc cccgaggttc acgaagtgga ctattactcc
    14941 gccctctacc gttggtacga ggatcatccc ctggcaggag agttgtaccg ttgggtctct
    15001 gtgacgcaag agggagaagt cgtcggccac ttgacggcgt tcccgcagtt ctacaggatc
    15061 gacggccggc gggtcgtcgc ccacacgccg ggagactaca tggtgcttcc ccagcacggt
    15121 ttccaggcta tcatgctgat gcgctccttt ttccgccagg tagagaattg cgtggtctgc
    15181 gatatggtac ccgcggtgat cggcatcgag acgcgcctgg gggccgaggt ggtcggcgac
    15241 ctgcagtacg cggcgaagct gatgaacgtc tcgcgcctac ccatgccctc cgtcccggcg
    15301 ccgctgcgga ggctcctcaa gctccccgag cagttcgcgc cggcccgagg gttcaccgac
    15361 aggcccggcg ccgaaccgca cgaggtggag gagcacgtcg ccccgccggc cccgcgcccg
    15421 cgcgcgccga tcccccgccc gctgcaggga ctgctgaacg ggggcctgcg ggcggtggac
    15481 gatgcactgg gcagcagcct cgcccgcgac ctcgcagtcg aggtgctcga tgggttcgac
    15541 gattccttcg acgacctgtt cgatagggtc gcggcggtgg tgccctgcac ggccgagaag
    15601 gatgcggcgt tcttgagctg gcgttatggt cccggttccc cccagtcccc cgtgaccgta
    15661 ctcggggtga gatctagtgg gaggctgctc ggctacgccg tgctcaaagc cgcctttcac
    15721 ggccaggacg gttacatact cgacctcacg gcgctccctg ggcgtcagga cgtcgtgctg
    15781 gccctgctgc gcgagacggt aagttccttc ggacggatgg gcgtccagat aataaggtac
    15841 aggttcgtag ggtcgcctac gtcgccgagg tccgccgacc tgctgcgtct tggcttcttc
    15901 tcccgcaggg gaagacgcca caagctgctt gtcaagttca cggataccaa cctgcgcggg
    15961 acgacgggcg acctcgtcaa ctggtcgctg tctttcggcg acggcgaagc tggcttctgg
    16021 ctcagataag gaggttgata catgatttct ggcaacggag atgcgagggc cgccatacgc
    16081 ggatggctcc aggacaacgt gaccggcggg cgcgacgtcc cgaacgacga gccgctcata
    16141 gacaacggcg taatgacgtc gctgcagacc gtcgagctcg tcgtgttcct cgaggacact
    16201 ttcggtatcg tcgtggagga cgaggagttc gacgaagaga acttcgggtc cgtcgaggcc
    16261 atcgccagac tcgtggagag caaagcggcg tgagaccacg tcctgaattc acgctccacg
    16321 atctgctcta caactccgcg gcgcgagacc cgcagagagc agcggtcgtg gacggcgata
    16381 cgaagtacac ctacgaggac ctgcaacgcc agagcggttc cctcggcgcc gcgctcgccg
    16441 aagcgggcgt aaggaggggc gatagggtcg gcgtctacct ggagaagtcc tgggaggcca
    16501 tcgtggcgat gctcgccgcc tcgcgcatag gcgccgccta cgtcaacgtg aaccccctgt
    16561 tcaaggcgcc ccaggtcgag tatctcgccg gcgactgtga cataagggtc atgatcgggg
    16621 acacctcgag gctcgaagaa ctccagccga agtcggtcca gaccgccttc tacaagggcg
    16681 cgaagccgga aggaccggcc gcccgtacgt atcaggacgt ggctgaggcc ttagagggcg
    16741 aaggcctcac ggaggacagg aacgtctccg agtccgacct ggggaccatc ctatacacct
    16801 cgggatccac ggggatgcca aagggtgtct cgacgagcca gcgcaatgtg gtggcggggg
    16861 cccagatcgt ctcgacgtac ctggagaaca agccggaaga ccggatactc tcggcgcttc
    16921 ctttgaactt cgacgccggg atgagccagt tcacgacctc cctgcgggcc ggcgcgacgt
    16981 tgtacctgct ccgatccagg ttgcccgggg acctgctgaa ggccttgcgc cggcatgaga
    17041 tcactggcgt aacgggcgtg ccgcccttgt gggcgctcct gatcctgggt gccaggtcca
    17101 tcagggagaa gccgcttact cacctgcgct acctcgctaa tacgggaggt cgcatacccc
    17161 aggccaacct cgacgagctc cgggatctgc tggagcccgc ggggaccagg atcttcctca
    17221 tgtacgggct tacggaagcg ttccgctcga cgtacctcgc gcccgaggag atccaccgtt
    17281 cctcgcccga gcagggtaac tgcatcggta aggctatacc gaacaccgag atcatggtca
    17341 taaccaaaga tggcgaagag gcggcgccgg gcgagccggg cgagctcgtc caccggggcc
    17401 ccaccgtggc gatgggctac tggggcaacg aggaggcgac gcggagggcc taccgcccga
    17461 accctctggc cccgccggag ctactcgata tcgagcgggt cgtctactcg ggcgacaccg
    17521 tagagcgcga tgcggaaggg tacctgtact tcctggggcg cgaagacgcc atgataaaga
    17581 accagggcta ccgcctgagc cccgaggagg tcgagaacct cctcctgagt tccgggctcg
    17641 tgcacgaggc ctgcgccttc ggggtggaga accccgccgt cgggcaggac gtgatcgccg
    17701 tgatctccct gcggaaaggc agcgaggagg gcgccgtgga cagggtcagg gagtacgcca
    17761 taaacaacgg gccaccctat atggtgccca aggagatact cgtcaaggac gagctaccca
    17821 agacgggttc gggcaagatc gaccgcaagg ggataagcag tgcttactca aacggataaa
    17881 gccaacgacc tggccggacg cttcggcggg gagagcgggg gcgagttcgc ccccgccggc
    17941 aagcccatct ccgggctggc tcgggagttc ggtacaccct tctacctcta ccacggggag
    18001 atgatctcgg agagggtcgg ccgcgtcagg gaggccctgg ggacggaggt ctcctactcg
    18061 gtaaaggcta atccgagcct cggggtatgc cagctcatag cctccgaaag ggaggccggg
    18121 gccgaggtcg cctcctcggg tgaactcgcg gtggccctgg ccgcagggtt cgagccccag
    18181 gacatcgtct tcgcgggccc gggcaagacc gacgacgagc tcaggcgcgc cgtcacggag
    18241 gggatcttcg ccgtcaacgt cgagtccgta cacgagatcg accgcctcgc ccgcctagcc
    18301 cgagaggcgg gcaagaccat cggtgtggga ctcaggatca accccgaagc ccagcttatg
    18361 ggctcgggga tgcgcatggg cgggacggtc ggacagttcg gcatggacca ggcggagctc
    18421 ggggagggtg tacggaagac gctcggtaga gccgaactcg tcctgcgcgg cgtccacgtc
    18481 tacacggcga cacaggtctt cgaggtgggg cccctcctgg agcactgccg caatattctg
    18541 gagatctcgc tggaggcggc cgacctggcc ggccggccgc tggagatgat cgacttcggc
    18601 ggcggcttcg gggtccccta cttcgagaag atgagcgagt tcgacctcga cggcttcgcc
    18661 gagggcttcc gcgacctgct ctcctccttc cgctccgatc cgcgcctccg aggatgccgg
    18721 tacctcttcg agttggggcg ctacctggtg gccgacgccg gcgtctacgt caccagggtc
    18781 gtcgacgtga aggagacgag gggcaagacc ttcgccgtca cggacggcgg catgaaccac
    18841 cacctcacgg cgacggggaa catgggccag gtgttcagga aggcctatcc gctcctcaac
    18901 ctcacccgca tgggcggggt gcccgaggag ggcatcgccg tagccggccc ctgctgcacg
    18961 ccgctggata tgttcggcaa caacatcacc ctggcagccc ccgaggaggg ggacctgatc
    19021 ggcgtcttct acagcggtgc ctacggctac tctgcctcca acctgggctt cctgagccat
    19081 cccgcgccag ccgaagtgct gctgtggcgg ggaggggccc acctcctgcg tcccggcggc
    19141 agcccggagg acgtcttgag cggccagaga ccgctctcga aaccggagtc cggcgtacca
    19201 ggctaggtgt cagcaagcca gcacgctcag gcttttagcc ttcgctcgtc agcacttcag
    19261 catttcagct ttcttggtgt aggttcgcta ctcttgctcg atagtgaatc attcgccact
    19321 acaaatagtc tctccgtagg agcttccctc cgccattcca ggcctagctg attgctgact
    19381 agagcaattt gcacgattat tgatcggctg agatactccg tgcatgagag cttattcgat
    19441 ggatctaagg gaaagagtgg ttgccgccgt cgaccgcggc acacctagaa aagagatagt
    19501 gcgcacgtta ggaatctc
//
LOCUS       DATMVF010000242         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_493679, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000242 DATMVF010000000
VERSION     DATMVF010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493679"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(633..1427)
                     /locus_tag="VN178_12475"
     CDS             complement(633..1427)
                     /locus_tag="VN178_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWS81828.1"
                     /translation="METLTSSDGTTIAFDRLGDGPPVIVVGGQLCDRALTRPTAEELA
                     KRFTVFNYDRRGRGGSGDTSPYALEREVEDIGALIAEARGKASVYGHSSGAALALHAA
                     AGGLPISKLVLHEPPYNPEGDEYRQRATQKEAEHIRTLLAEDRPDHALEYFWRSIGMP
                     QEMVDGLRQTPRWAELEAMAPTMAYDSEVMGDIGRGGAVPTDLAGRVRSETLVLVGGA
                     SPEWMIDVARQVADALPHGRYRVLEGQEHVVPPEILVPVLAEFLAG"
     gene            complement(1744..2325)
                     /locus_tag="VN178_12480"
     CDS             complement(1744..2325)
                     /locus_tag="VN178_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS81829.1"
                     /translation="MTAPYTFDVFSSLDGYGSYDPPGDWGGYWGKQGPDLLDHRLALY
                     DDEQRMVFGATTFRQFVSMLASSTEESEVRDPWVTRMRSMPATVVSTTLEEPLDWPDA
                     TVVNGDAVDVVARLKEESEVPLRSHGSLSMNRALMAAGLVDRVQVTIFPVITGQTGAD
                     PIFRGAADFDLELIEHRTLDGHTQELVYRPTLH"
     gene            2594..>2896
                     /locus_tag="VN178_12485"
     CDS             2594..>2896
                     /locus_tag="VN178_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81830.1"
                     /translation="MKIEPFALERWMTAHELSASYDIAESGILPLSTNDLLAFEEPEE
                     RQAILEGLLETPLGYSEASGSLELRTTLAGTYRERGPENILVTTGAIEANFLLFNVL"
BASE COUNT          515 a          932 c          903 g          546 t
ORIGIN      
        1 tccagcaccg tcatagcccg tccgatgacc accatatcgt cgtggagagg tttgatgtgc
       61 ggtggcagaa actggcgtag cagtcccatg gtgtccatga cgtcgccaac caccgccgtg
      121 aaaagatcgc gacggatcgt gtcgtatagc tcttcgtccg agccccagtg catcacgttc
      181 tactaccttt cccttgtccg tcacggcgct aatgaaagtg taaggtgagc agatggcacg
      241 cgccatgagg atcatcgggc cgccccgcag gcgtgtatat tactgccgct cctggcaagc
      301 tggccgtagg ttcaggaagg atattccacc agctcaacgg gcgacctcct cggacttagt
      361 tcacggtgga ctcgcgcagt agacaacgac ctcagtctcc ttgcggggga tcagctcctc
      421 cgcttggtcg gcttgacttg gcggcaggtt aatcgcaccc gacaggtggc tgtaggcccg
      481 tgaaaatatg gttcagaccg gccggaggtc actcgccacc catattctcg cagtgaaaac
      541 agcctcggcg gcagctcgat tttcatggct gcgggatggc ccggccaggc tatgggtcac
      601 ttaggtgaat aagcgtcgag gtagctccgt tctcatccgg ctaggaactc ggccagcacc
      661 ggtacgagga tctcgggggg cacgacgtgt tcctggccct ccagaaccct gtaccgcccg
      721 tgcggcaggg cgtcggcaac ctgcctggcg acgtcgatca tccactccgg gctcgccccg
      781 ccgacgagga cgagggtctc gctcctcacg cggccggcca agtcggtcgg cacagccccg
      841 cccctgccga tgtcgcccat cacctcggag tcgtaggcca tcgtgggcgc catggcctcc
      901 agctctgccc acctcggggt ctggcgcaac ccatccacca tctcctgggg catcccgatg
      961 gacctccaga agtactcgag cgcgtggtct gggcggtctt ctgccagcag ggtcctgatg
     1021 tgttcggctt ccttttgcgt ggcgcgctgc cggtactcgt cgccctcggg gttgtagggc
     1081 ggctcgtgca ggacgagctt ggagatcggc agacctcccg ccgctgcgtg gagggcgagg
     1141 gcggcgccgg aggaatggcc gtagacagaa gccttccctc tggcctcggc gatcagcgcg
     1201 ccgatgtctt cgacctcccg ctcgagagcg tacggcgagg tgtcgccgct gccgccccga
     1261 ccccggcggt cgtagttgaa cacggtgaag cgcttggcta gttcctcggc cgtcggacgg
     1321 gtcaaagccc ggtcgcacag ttgcccgccc accacgataa ctggtggacc gtcgcccagc
     1381 cgatcgaagg cgatcgtggt gccgtcgctc gaagtcagcg tttccatggg gacgctctcc
     1441 tccctcgtcc ggtagctcac gcccgtccgc ttgaagcata cggcaatggt ctcggggtcc
     1501 accatcgatt cgagttttgg cgaaagtata gtgggatgct agcagcgaca cggcgcggaa
     1561 gatcatgcca cagcatgggc gcgacaacac gcttgagctg ctcaacttcg cagaataccc
     1621 cttccaagca ctccggtgaa tagtgccctc cgagatcctc cctacacgct caccacgtcc
     1681 gccgatcctg ctcgcagagc tgtggacgtc cacgtccgtg atcctcttcg aaacccttac
     1741 gggtcaatgg agggtgggtc ggtagacgag ctcctgggtg tgcccgtcga gggtccggtg
     1801 ctcgatcagc tcgaggtcga agtcggccgc accccggaag atcgggtctg ctccggtctg
     1861 gccggtaatg accgggaaga tcgtcacctg gacgcggtcg acgaggccgg cggccatcag
     1921 cgcccggttc atcgacaggc tgccgtggga gcgcaacggc acctcggact cctccttgag
     1981 gcgggcgacg acgtcgacgg catccccgtt cacgacggtc gcgtctggcc agtcgagggg
     2041 ttcctccagg gtcgtcgaca ccacggttgc cggcatactc ctcatccggg tgacccaggg
     2101 gtcacgcacc tcggactcct cggtgctcga ggccagcatc gacacgaact gccggaacgt
     2161 ggtggcgccg aagaccatcc gctgctcatc gtcgtacaag gcaaggcggt ggtccagcag
     2221 atcggggcct tgcttgcccc agtagccgcc ccagtcgccc ggggggtcgt aggagccgta
     2281 gccgtcgagg ctggaaaaga cgtcgaaggt gtagggcgcg gtcatgtcgt tctcctcgag
     2341 tggttcgcgg tggtcgtcag cttcatagtg ggttcccttc tcatctaagt cgatgaggtg
     2401 atcttaatgg aactcggaac cccgaccacc agtaaactgc ggagaacgtg cataaggcga
     2461 agttcgcaga atccgataaa ggcgaagttc acagtattcc tcttccccga cgttagggga
     2521 ataaggtgtc gcagtcccct tgcggataca ctgaagcgtt gttctggcgc tccacgaggg
     2581 aagggacccc gctgtgaaga tagagccgtt tgccttagaa cgttggatga ccgcccacga
     2641 gctctcggcc agctacgaca tagcggagag cggcatcctc cccctctcta ctaacgacct
     2701 gctcgccttc gaagagcccg aagaacgcca agccatcctg gaagggctgc ttgagacccc
     2761 gctcggatac agcgaggcct ccggctcgct cgagctcagg accaccttgg cgggcacgta
     2821 ccgcgagcgc gggcccgaga acatcttggt caccacgggc gcgatcgagg ccaacttcct
     2881 gctgttcaac gtcctg
//
LOCUS       DATMVF010000243         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_674321, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000243 DATMVF010000000
VERSION     DATMVF010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674321"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1673)
                     /locus_tag="VN178_12490"
     CDS             complement(333..1673)
                     /locus_tag="VN178_12490"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWS81831.1"
                     /translation="MQAEREAQERVVRPLPEGTLWVRFVFVDHAGIPKAKAVYRDGFD
                     GRARAGVGLAKGVLALDPSGALHPESGLSPVGECRLIPDLSTLTPLPFARGQAMVTVD
                     MTEPDASTPWEGCPRSALKRVLERLSDRGYRAFASNEVEFYVWDADGPLDKTPYAGSY
                     ALTPAADFVAELAQTLEEMGIRPEQCHAEVGQGNLELSVGEDEALAAADKRVMVLEAI
                     RGVAHRMGLGTTMAPKPYLEGAGNGHHLHVSLYEGDTPVLFDASGAPSSSGSGFVAGI
                     LEHMPAVMAFTAPSPNSYQRLAPGMWASAYAAYGLDNREAAVRLASPVAGREAATANV
                     EIKPVDVTSNPYLALAAVLAAGLDGMDRGLDPGEPTMVDPATLCDEERASRGILPLPA
                     SPDEALDALEEDRVLIEALGEPLVRTHVAVARAQAKIARELPPEDVASTAAMLY"
     gene            complement(1846..3336)
                     /locus_tag="VN178_12495"
     CDS             complement(1846..3336)
                     /locus_tag="VN178_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWS81832.1"
                     /translation="MSTEASSTESPHLRRELRLWEAVGISLALMAPSMAANINPQGTA
                     DTGVGRAVPLAFALATIGVLLIAWTFVRLTQRFNHAGSVYGFVGATLGARAGVISGWA
                     LMGTYTFYGVVTSTAAGIFGAEFLDALGIWTNQPGWAPFLVGAVALAGVWALAASNIR
                     GGTRVLLVIEGTTVALILIVSVVILIRLATGTAPNGNTLDFSVFSVPGGIGTSALFLG
                     VVFGFLSFAGFEAAATLGEEAQEPRRNIPRAILGVAIFGGLYYVFVTAIEVMGFGTDR
                     QGVEDFIASGSLLGDLGSQFVAGWVGELITIGAAISAFGCALACIVGAARLLYALSRD
                     EVGPASLGAVSSRSGVPARSTATVSIGAYVIIALGWLVFGVVPFDLFVASGTIGTLIL
                     LLVYALATVGAAKLLFLSGERQVAAWEVVVPFLALVVIGYTLLRNIYPYPTDAAAWYP
                     IIATLWVIAGILLTFVRYAATQRAGERLMAEEGLAAETGSRTEGNR"
     gene            3559..3852
                     /locus_tag="VN178_12500"
     CDS             3559..3852
                     /locus_tag="VN178_12500"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWS81833.1"
                     /translation="MIATFKDEGTEDVFDGRNTKNARKACPQNLWRVAFRKLDQINQA
                     TELNDLRVPPGNRLEGLSGNREGQHSIRINGQYRVCFTWTERGAEEVEIVDYH"
     gene            3866..>4133
                     /locus_tag="VN178_12505"
     CDS             3866..>4133
                     /locus_tag="VN178_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWS81834.1"
                     /translation="MRIPKDRVPTSPGVMLREEFLEPLGMTQVELAQRIRVPYVRVNE
                     LVNGKRRITPSTALRLAKAFGTSPEFWLNGQLALDLYRVINDKQE"
BASE COUNT          811 a         1345 c         1296 g          681 t
ORIGIN      
        1 cggctcgtag cgcgacctcc tcctggctgg ccgcgtggcc agccgccgag agtgccaacg
       61 ccccccagaa cgcgccgcac agctggtcct tctgcggcat cgccctctcg tggagcagga
      121 cgaagtcccc gggaccgggg aggatctcac ccgctccgac tagcggaccg atcacgctgc
      181 gcctcctttc acattaggtt acaggcttca ggcatcagct tgccgatagc cggcggagct
      241 cttgcggaca caccaacccg cgaggtggta cgtatcgcct cccggtctgg ctttctccgg
      301 acgattcctg aagcctgata cctgtagcct ttctagtaga gcatcgcggc cgtggacgct
      361 acatcttccg gtggaagctc tcttgctatc tttgcctgtg cccgggccac ggccacgtgg
      421 gtgcgcacga gtggttcgcc cagcgcctct atcaggacgc gatcttcttc cagcgcgtcc
      481 agagcttcgt ccggggatgc ggggagcggc aggatgcccc tggaagcgcg ctcctcatcg
      541 cagagcgtgg cagggtccac catcgtcggc tccccgggat cgaggccacg atccataccg
      601 tccaacccgg cggccaggac cgcggcgagg gcaagatagg ggttcgacgt gacgtcgacc
      661 ggtttgatct cgacgttggc ggtagcggcc tctctgccgg ccaccggcga ggcgagccgc
      721 acggcggcct ccctgttatc caggccgtat gcggcgtaag ccgaggccca catgccaggg
      781 gcgaggcgct ggtaggagtt gggcgacggt gctgtgaacg ccatgacggc gggcatgtgc
      841 tccagtatcc cggccacaaa gcccgagcca gagctgctcg gggctcctga ggcgtcgaag
      901 agcaccgggg tatcaccctc gtagaggctc acgtgcaggt ggtgaccgtt gcctgcccct
      961 tccaggtacg gtttcggggc catcgtggtc ccaagcccca tccggtgggc cacgcccctg
     1021 atcgcctcca gcaccataac gcgcttgtct gcggcagcca acgcctcgtc ttcacccacg
     1081 gagagctcga ggtttccctg cccgacctcg gcgtggcact gctccggccg gatacccatc
     1141 tcctccagag tctgtgccag ctctgcgacg aagtccgccg ccggggtcag cgcatagctc
     1201 cccgcgtacg gggtcttgtc cagaggaccg tcggcatccc acacatagaa ctcgacctcg
     1261 ttggaggcga acgccctgta acctctatcc gagagccgtt cgagcacccg cttgagggcg
     1321 ctacgcgggc agccctccca gggcgtgctc gcgtccggct ccgtcatgtc gacggtgacc
     1381 atcgcctgcc cgcgggcgaa gggcaggggg gtgagtgtcg agaggtccgg gatcagccgg
     1441 cactccccta ccgggctcag gcccgactcc ggatgcaacg ccccgctcgg atccagcgcc
     1501 aggactcctt tggcgagccc aaccccggcc ctcgccctcc cgtcgaaacc atctctatag
     1561 acggccttcg ccttggggat gccggcgtgg tccacgaaca cgaatcggac ccacagagta
     1621 ccttctggca aaggccgtac cactctctcc tgcgcttcgc gctcggcttg catctcaccc
     1681 tccaacattc gctgaatacc tgttctcggg cctcgctcaa tatctcatgc cagagccata
     1741 cgttctagag catcgacaac aatgagccaa aaagctgaat tgccgacaag cgaaggcgaa
     1801 gcctgagcgt gctgatttgc tgacaagctg ccgcaggcag cgtgcttacc tgttgccctc
     1861 cgtccgggag ccggtctctg cggcgaggcc ctcttcggcc atcagcctct cgcctgcccg
     1921 ctgcgtcgcg gcgtagcgga cgaacgtaag caggattcca gctatgaccc atagtgtggc
     1981 aatgatcggg taccatgcag cggcgtctgt cgggtaaggg tagatgttgc ggaggagcgt
     2041 atagccgatg acgacgagcg ccaggaaagg caccacgacc tcccacgcgg ctacctgccg
     2101 ctcgcccgag aggaagagca gcttcgccgc ccccacggtc gcgagcgcgt acacgagaag
     2161 caggatcagg gtgcctatag tgcccgaggc cacgaacagg tcgaatggaa ccacgccgaa
     2221 caccagccag ccgagcgcga tgataacgta agcaccgatg gagacggtgg ccgtagaccg
     2281 cgccgggacg ccgctccttg aggagaccgc gccgagcgaa gcggggccga cctcgtctcg
     2341 ggagagtgca tacagcaggc gggcggcccc cactatgcag gccagcgcgc agccgaaggc
     2401 gctgatcgcg gcgccgatgg ttatgagctc gccgacccac ccggcgacga actggctgcc
     2461 gaggtcgccg agaagcgagc cggacgcgat aaagtcctcg actccctgcc tgtccgtccc
     2521 gaagcccatc acctcgatgg cggtgacgaa gacatagtac aggccgccga agatggcgac
     2581 gccaaggatc gcacgcggta tgttgcggcg gggctcctgc gcctcctcgc cgagcgtcgc
     2641 ggcagcctcg aacccggcga aggagaggaa cccgaacacc acgccgagga agagcgccga
     2701 tgtacctata ccacctggta ccgagaacac agagaagtcc agggtgttgc cgttcggggc
     2761 cgtaccggtc gcgagacgga tcaggataac gacgctgacg atgaggatga gcgcgaccgt
     2821 cgtaccctcg atcaccagga gtaccctggt acccccacgg atgttcgagg ccgcgagcgc
     2881 ccacaccccg gcgagggcga ccgcacctac caggaacggc gcccaccccg gctggttcgt
     2941 ccagataccc agggcgtcca ggaattccgc gccgaagatg ccggccgcgg tagacgtcac
     3001 cacaccgtag aaggtgtacg tgcccatcaa cgcccacccc gaaatgacgc cggccctggc
     3061 tccgagcgtc gccccgacga agccgtacac gcttccagcg tggttgaatc gttgggtcaa
     3121 ccggacgaag gtccaggcga taagcagaac tccgatggtc gcaagcgcga acgcgagcgg
     3181 aacggcgcgt ccgacgccgg tgtcggcggt tccctgcggg ttgatgttgg ccgccatcga
     3241 aggcgccata agggccagcg agatgccgac cgcctcccac aggcgcaact cacgccgcag
     3301 atgtgggctc tcagtgctac tagcttctgt agacatactg actccttcgc tcagtggtgc
     3361 cgggtacaga agatcgcgga ctggttcaga ccgattccac aagcacccaa ccgcttcgca
     3421 gggcgacggc catggtttgc cctcacgctt acaatatctc acgccacggg cctctattcc
     3481 atagcgttca gcgagggttg ggggctactt ctcatgaaat cattaacacg tataacgtaa
     3541 tgcgttatac tggagaaggt gatcgctacg ttcaaagacg agggtacgga agacgtcttt
     3601 gacggacgga acactaagaa cgcccgaaag gcgtgtccgc agaacctctg gcgtgtggcc
     3661 tttaggaagc tggaccagat caaccaggcc acagagctga acgacttgcg ggttccgcca
     3721 ggcaacaggc tggaagggct aagtggaaac cgagaaggcc agcacagtat aaggatcaat
     3781 ggccagtaca gagtgtgttt tacgtggacg gaacgcggag ccgaagaggt ggagatagtc
     3841 gactaccatt aaggaggtga ggggagtgcg tataccaaag gacagagttc caacgtctcc
     3901 cggagtgatg ttgcgcgagg agttcctcga acctctgggt atgacacagg tcgagcttgc
     3961 gcagaggatc agggtgccct acgtcagggt caacgagctc gtaaacggca agcggcgcat
     4021 cactccctcg acggcgttac gtctggctaa ggcgtttggc accagtccgg agttctggtt
     4081 gaacgggcag ctcgcgctag atctctaccg ggtgatcaac gacaagcaag aga
//
LOCUS       DATMVF010000244         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_493809, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000244 DATMVF010000000
VERSION     DATMVF010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493809"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VN178_12510"
     CDS             <1..921
                     /locus_tag="VN178_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81835.1"
                     /translation="CEHGNQFDSENRVGDYHDPLDTPLGHHVVMDGTRRIAPFGEISP
                     GLDLSEIKMVYPLVAIPAWIVSRYFYHWAGKVARYLLMPLLVAFAIYKVVAFLISRAT
                     NGQASLLFGSYRELPQIHQLFLDTALFLLLLLGIFAIFFLVARHAVRKTLRAVSPGGT
                     PHYSPAEASQRRIRAVLDGDARAPMDPSFDPTTADVFVSGHTHLPSLSETERPDGSKA
                     VLVNSGCFLRQLQPITPRPKGPPIFVSKFVLTHVRVFAKGEKLRVELWEQPKPAQQTL
                     TRIERLVSIGRRPPQPPKGAKPRLVASATL"
     gene            1014..1400
                     /locus_tag="VN178_12515"
     CDS             1014..1400
                     /locus_tag="VN178_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWS81836.1"
                     /translation="MSDQELDQQFVESQRERLVGLRDELVRIRDGMSADERDLGETEG
                     ETTLDSGDLSQDMFTREMDASIGEQVERRLREVDRALRKIEEGTYGLSDDSGESIPRG
                     RLEAVPEAVRTVEEQQRFEQERRPPI"
     gene            1527..2396
                     /locus_tag="VN178_12520"
     CDS             1527..2396
                     /locus_tag="VN178_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWS81837.1"
                     /translation="MDHRSSGGETREPIFRSDLEGPRPEPRYEPLRPEGGLWSVIKRW
                     IAPLAAVGLLLAKFKGLALLLLKVKFLGTALTMLISIGAYALLFPVWFAVGIVVLIWV
                     HEMGHVLQLRREGIPASAPMFIPFLGAFVAMKQMPKDALAEARVGLAGPVLGTLGGLA
                     TLGLYAATREPLFLGLAFFNFIINLFNLAPLLPLDGGRAVGAMSPVFQLLGLVVMVAL
                     FFVAPIMAFIALLGLPELWHRWKTRNTPEGKAYYDIPLRQRVGVGMVYLGLIVVLGLL
                     TGATFVPDPTVLQ"
     gene            2398..3567
                     /locus_tag="VN178_12525"
     CDS             2398..3567
                     /locus_tag="VN178_12525"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HWS81838.1"
                     /translation="MRTILYTGKGGVGKTSVAAATALKASLAGKKVLVMSTDPAHSLA
                     DVFDTEIGSDPKEMAPGLWAQEMDHTSMIEENWSEIQGYMTTLFEWQGADTLAAEELA
                     MLPGMDELFGLLMVRRHNQEGRYDALILDAAPTGETLKLLSLPDHMSWYVEKIFPIQR
                     RAAKLVRPFANRARSLPPLPEDSVFAAGQRFYQAIASVEEILTDRDSASVRLVLNAEK
                     MVIAEARRAYTYLNLYDYGVDALVVNRLLPESVSDPYFARWHEAQVRHMGTIEESFDP
                     IPILTARLFDREMFGLEALAALAEDVFEDTDPLGVLFRGATHDIVRNGDGYDVVFNLP
                     LAEKRNVDLSKKGAELLVKVGNYRRSILLPDALARLSAAGASFEGGSLKVRLKDE"
BASE COUNT          668 a         1091 c         1143 g          674 t
ORIGIN      
        1 tgcgagcacg gcaaccagtt cgactccgaa aacagggtcg gggattacca cgatcctctg
       61 gacacgccgc tggggcacca cgtcgtgatg gacggcacca ggcgcatcgc gccgttcggt
      121 gagatctcgc cggggttgga cctttcagag ataaagatgg tctacccgct ggtggcgatt
      181 ccggcctgga tcgtgagccg ctacttctac cactgggccg gcaaggtcgc ccgttacctg
      241 ctcatgccac tgctcgtggc tttcgcgatc tacaaggtgg tcgcgttctt gatctcccgc
      301 gccaccaacg gtcaggcgag cctgctgttc ggcagttacc gcgagctgcc ccaaatccac
      361 cagctctttc tcgataccgc ccttttcctg ctcttgctgc tcgggatttt cgcgatcttc
      421 ttcctggtgg cgcgccacgc cgtccgtaag acgttgaggg cggtgagccc tggcgggacg
      481 ccacactact cgccggcgga ggcctcccag aggaggatcc gggctgtcct cgatggtgat
      541 gccagggcgc cgatggaccc ctcgttcgat cccacaacgg ccgacgtctt cgtctctggg
      601 cacacccacc tgccttcact ctccgagacc gagaggcctg atggtagcaa ggcggtgctg
      661 gtgaactccg gatgcttttt gcggcagctg cagcccataa cgccccggcc aaagggaccg
      721 cccatcttcg tctcgaagtt cgtgctcacc catgtgcggg tgtttgcaaa gggagagaag
      781 ttgcgtgtcg agctctggga gcagccgaag ccggcacaac agaccctgac ccgtatcgag
      841 cgcctcgttt ctatcgggcg tcggccgcca cagccgccca agggggcaaa gcctcgcctc
      901 gtggcctcgg ctaccttgta gtcggccgcg tagcgtgtct tttcgggagt ggtagggtat
      961 acatgaacag cattatttct tatggtcgac tggaagagat ccggaggaaa taggtgtctg
     1021 atcaggagtt ggaccagcag ttcgtagaga gccagcgaga gaggctggta ggcctgcgcg
     1081 acgaactggt gcgcatacga gatggcatgt ccgctgacga aagggacctc ggcgagaccg
     1141 agggggagac tacgctcgac tctggcgacc tgagccagga tatgttcacc cgcgagatgg
     1201 acgcctccat aggggaacag gtcgagcgcc gcctcaggga ggtcgaccgg gcgctgcgga
     1261 agatagaaga aggcacctat gggctctccg acgacagcgg ggagtccata cccaggggaa
     1321 ggctcgaggc cgtacccgaa gcggtaagga ccgtcgaaga gcaacagcgt ttcgagcagg
     1381 agcgacgccc cccgatttag agcattctgc gatcagcggt cagccgtcag ctgacacaaa
     1441 gagctgagag ctgaaagctg agcgctgaca gcgcgcgaag tggtccaaga ccacttcgcg
     1501 cacttctctg gcggtataat ctgcctatgg atcatcgttc gtcaggcggc gagacaagag
     1561 agcccatctt ccgcagcgac ctcgaagggc cgagaccaga acccaggtat gagcccctga
     1621 ggccagaagg cggcctgtgg agcgttataa agcgctggat cgcgccgctc gccgcggttg
     1681 ggctcttgct cgccaagttc aagggcctgg cgcttctcct tctcaaggtc aagttcctgg
     1741 gcactgcgct gaccatgctc atcagcatag gggcttacgc cctgctgttt cccgtctggt
     1801 tcgcggtcgg gatagtcgtc ctgatctggg tgcacgagat gggccacgtc ttgcagctaa
     1861 gacgcgaagg aatcccggct tcggccccta tgttcatacc ttttctcggg gccttcgtag
     1921 cgatgaagca gatgcccaaa gatgccctgg ccgaggcacg cgtcgggctt gccggccccg
     1981 tgctcggcac gcttggagga ctcgctactc tcggtctcta cgccgccacg cgggaaccgc
     2041 tgtttttggg gctcgcgttt ttcaacttca tcataaacct gttcaacctc gcaccgctgc
     2101 tcccgctcga cggcggcagg gccgttgggg cgatgtcccc ggtcttccag ttgctaggtc
     2161 tcgtcgtgat ggtcgcgttg ttcttcgtgg cccccatcat ggcctttatc gcgctgctcg
     2221 gcctgcctga actctggcac cgctggaaga cacgcaacac gccggaaggc aaggcgtact
     2281 acgatattcc actacgccag cgcgtcgggg ttggcatggt ctacctgggt ttgatcgtgg
     2341 tgcttggatt gctgacgggg gccacgttcg tgccggaccc gacggtgctc cagtagcttg
     2401 cgtacgatcc tctacactgg aaagggcggc gtcggcaaga cgagcgtcgc cgcggcgacg
     2461 gccctgaaag cttctctcgc gggaaagaaa gtgctcgtga tgagcaccga tcctgcccac
     2521 tcgctcgccg acgtcttcga cacggagata ggatctgatc cgaaggagat ggcacccggc
     2581 ctgtgggccc aagagatgga ccacacctcc atgatcgagg agaactggtc cgagatacag
     2641 gggtacatga ccacgctgtt cgagtggcag ggcgcggata cgctcgcggc cgaggagttg
     2701 gccatgttgc ccgggatgga cgagctcttc gggctcctta tggtcaggcg acacaaccag
     2761 gagggccgct acgacgccct gatcctggac gccgcgccga cgggcgagac gctgaagctc
     2821 ctcagtcttc ccgaccacat gagctggtac gtggagaaga tcttccccat acaacgccgc
     2881 gccgctaagc tagtcaggcc atttgcgaac agggcgaggt ccctgcctcc gctgcccgaa
     2941 gacagcgtct tcgccgcggg gcagaggttc taccaggcta tcgcgagcgt cgaggagatc
     3001 ctgaccgacc gcgatagcgc atccgtgcgc ctggtgctca acgcggagaa gatggtcata
     3061 gcggaggcgc gccgcgccta cacctacctc aacctatacg actacggtgt cgacgcccta
     3121 gtggtcaacc ggctgttgcc ggagagtgtg agcgaccctt acttcgcgcg ctggcacgag
     3181 gcgcaggtgc ggcacatggg gacgatagaa gagtctttcg accccatccc catcctgacc
     3241 gcacggctct tcgaccgcga gatgttcggc ctggaggcgc tcgccgcgct cgccgaggac
     3301 gtcttcgagg acacggaccc tctgggggtg ctattcaggg gcgcaacgca cgacatagta
     3361 aggaacggcg acggatacga cgtcgtcttc aatctcccgc tcgccgagaa acggaacgtg
     3421 gacctgtcga agaagggggc cgagttgctc gtgaaggtcg gcaactaccg gaggagcatc
     3481 ctgctgcctg acgccctcgc gcgtctctcc gcggccgggg cgagcttcga gggagggtcg
     3541 ctgaaagtga ggttgaagga tgagtgaaga gaaacg
//
LOCUS       DATMVF010000245         6444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_178707, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000245 DATMVF010000000
VERSION     DATMVF010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178707"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VN178_12530"
     CDS             complement(<1..475)
                     /locus_tag="VN178_12530"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWS81839.1"
                     /translation="MFLEHRGERPKVHDGAYVAPNATLCGDVTVGENVRVMFGAILTA
                     EGGPVDVGANCVVMENAVVRGTKRHPTSIGDNVVVGPRAHLVGCRVGDNAFLATGTTV
                     FNGAVVGEGAEVRVNGTVHLRTVLPPETVVPIGWVAVGDPAEVLAPGEHERIWELQ"
     gene            728..1552
                     /locus_tag="VN178_12535"
     CDS             728..1552
                     /locus_tag="VN178_12535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS81840.1"
                     /translation="MAIEVTFSEDPEWVLTEAEVFLASEPVLHNLILVLLGERLAYPE
                     PGRYWLAKDGETVVGVVFQSPLDFAATLTPMGPEAAAGMVDAIVETGVALPGVIGEAA
                     TAARFAGQWTERHGSAAFPVEGQRIYEMARVPERVEVGGRLRNASLHDRDLIVAWMRG
                     FYADVGQEARGHEPVVERRLAAGQFWLWEDGEPVSMAANSNPVEGVLRVQAVYTPPDR
                     RNRGYAGACVGNLSRRMRDEGYRCILYTDLGNPTSNSVYRRIGFNAVAEALWYRFE"
     gene            1678..2157
                     /locus_tag="VN178_12540"
     CDS             1678..2157
                     /locus_tag="VN178_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWS81841.1"
                     /translation="MTTETPTLRRAARVLLLDENNRVLLVRLAYRGKRWWAAPGGGLE
                     YDETHETAARREVAEETGFELDELGPWAWRREDVFRFEGRLYRQRERYFIASVPAFEP
                     HPQVIDAEEAKAFDGLRWWTLGELEATDERFAPANLPALVRELVEEGPPEHPILVSV"
     gene            complement(2271..3083)
                     /locus_tag="VN178_12545"
     CDS             complement(2271..3083)
                     /locus_tag="VN178_12545"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS81842.1"
                     /translation="MIDFKLMEDKYQEVDDGPDLNEFYDPRLVAIYDTANPVAEYERF
                     YLDLASKLSASSIIDIGCGTGLLTCELAKRGHRLIGVEPSKAMLEVARRRACGARVEW
                     IEGDVLGLDELQADLAIMTGHVAQFFLDDAGWHSTLVAIRKALRPGGHIAFESRNPLV
                     QPWANKMIKDHVDWPAQTSRRRVDDSMAGRIEWWTEILEIEDDRVRTAIHYLFTRSGE
                     VLVSNNELRFRTRAELSRSLSDAGFSVESVFGDWDSRPADVASRELIFVAAS"
     gene            3317..4162
                     /locus_tag="VN178_12550"
     CDS             3317..4162
                     /locus_tag="VN178_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS81843.1"
                     /translation="MRETDLELRDGRTLHVYDTGSDDADGRLAVFWHHGSPNIGSPPQ
                     PLFAAAEELGIRWVSFDRPGYGGSTPHPGRNVASASAYVSTIADALGIDRFAVMGHSS
                     GGSHALACAALLQERVLGAVVVAGMAPYHAEGLDWFEGFGPGGEAQLRAAAAGRAALE
                     KRLAESDDEPDFTPEDEAALAGEWSWFIEVVRPALAGGMGGFIDDDLAGVGEWGFDPA
                     GIVAPVLFLHGGQDRVVPSSHSEWLARRCPTSELWLYPEDGHISVLNFSRAAMAWLQE
                     HADQG"
     gene            complement(4422..5663)
                     /locus_tag="VN178_12555"
     CDS             complement(4422..5663)
                     /locus_tag="VN178_12555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81844.1"
                     /translation="MQTETRWKRLLALEVSAFGFFGLFWGCFAVLLADLSGALGLSPG
                     PLGVALFVGAGASIAAMAALGWASDRLGRRVYLILTTCAFGMGIAGLAFSGSYTVLLA
                     TLVVLYSFSGLYDVGINALAVDLERVSGRRFMSYLHAAFSGGAMAGAVGAGALLQAGV
                     DYRLVYLALLLPLISLVAAFTAARFPRPGTRGTRDERSHSGTSPAADSGDKGSAGRWD
                     LYRSGPLLLVAAIACLGLLAEGEMEHWSGIYLRDTLGLAAIVGGSGVAVFYGAQALGR
                     LAIGWIVGRVGNRRTLLGSGLLTAGGMLLALATTSSALVIGGFLLVGMGLAAVAPVAF
                     SVAGELVPERAGSAISVVTTFGYGGFLLGPPLVGGLAEVAGLRGALGVVAVAGLAIFV
                     LSLRLREPKADTATEDGVEAP"
     gene            5806..6207
                     /locus_tag="VN178_12560"
     CDS             5806..6207
                     /locus_tag="VN178_12560"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWS81845.1"
                     /translation="MRARPGYEDERAVAFLDGYPRAYGYTLVAPKEHREQVTGDFTME
                     EYIGLQRLVYRVSEAVREEVGAERMYIFTYGSNQGNSHVHWHVVPLPPGVPYEDQQGA
                     WTSWSKGVLEIPQSEMASLAACIGRRVRGKT"
BASE COUNT         1173 a         2128 c         2049 g         1094 t
ORIGIN      
        1 cctgcagctc ccagatccgc tcgtgctcgc cgggcgccag gacctccgcc gggtcgccaa
       61 cagccaccca accgatggga accaccgtct ccggtggtag caccgtcctc aggtgcacgg
      121 tcccgttgac gcgcacctcg gccccctcgc cgacgaccgc cccgttgaag accgtggtgc
      181 ccgtggccag gaaggcgtta tccccgaccc ggcagcccac gaggtgcgcc ctcggtccca
      241 ccaccacgtt gtccccgatg ctcgtggggt gacgtttggt gccgcgcacc accgcgttct
      301 ccatcaccac gcagttggcg ccgacatcca cgggtccgcc ctcggcggtg aggatcgccc
      361 cgaacatgac tcggacgttc tcgcctacgg ttacatcgcc gcagagggtg gcgttgggcg
      421 ccacgtaggc gccgtcgtgg accttcgggc gttcgccccg gtgttcgaga aacacggtta
      481 acctcctgtc tgttacccac tggcgtaggt ctttgtaccc ggcggcccga ggctagcaac
      541 ccgtcacggg ccagatccct ccgtcctgag ctgcgtgttg gcgtcccagc cacagggcga
      601 gtactggtca gcttgcacgg tagccaaacc tcgcgggttc tgccggtcta cagccattcg
      661 tatcgagcaa tgcgcgaagc gaattctcgg ccaagaattc gcaacggcgg cgtgcaacaa
      721 tctcgccatg gccatcgaag tcacctttag tgaagatccc gagtgggtgc ttaccgaggc
      781 cgaagtattc ctggcttcgg agccggtact gcacaacctc atactcgtgc tcctaggcga
      841 gcgcctcgct taccccgagc ccggccgata ctggttggcg aaagacggcg aaaccgttgt
      901 gggcgtggtc ttccagtctc ctctggactt cgccgctacc ctcacaccca tggggcccga
      961 agcggccgca gggatggtag acgcgatcgt ggagacgggt gtcgccctcc cgggggtgat
     1021 cggcgaggcg gcgacggcag cacgcttcgc cgggcagtgg actgagcgcc acggttcggc
     1081 ggcttttccc gtcgagggac aacgtatcta cgagatggct cgggtgccgg aaagagtcga
     1141 ggttggcggt cgccttcgca atgcgtctct ccacgaccgc gacctcatcg tcgcctggat
     1201 gcgcggcttt tacgccgacg tcggtcagga agcaagaggc cacgaacccg ttgtcgagcg
     1261 gcggctggca gccgggcaat tctggttgtg ggaagacggc gagcccgtgt caatggcggc
     1321 caactcgaat cccgtagaag gtgttctccg cgtacaggcc gtctacacgc cgccggacag
     1381 acgcaatcgc ggctacgccg gagcgtgcgt cggcaatctc tcgcgacgta tgcgcgacga
     1441 gggataccgc tgcatcctct acaccgacct gggcaatccg acctccaact cggtgtaccg
     1501 ccggatcggc ttcaacgccg tcgccgaagc cctgtggtac cgattcgagt gagacattcg
     1561 ggttcgtagc gctccgacgg gacgtctttc ggcaatcggg tactgataat ccagagtgga
     1621 tggtccgcta cttcgaactg ctccgggatg tcccgactcg tgtaggctag gtggacgatg
     1681 accacagaga cgccaaccct ccggagagct gctcgcgttc tcttgctcga cgagaacaat
     1741 cgggtgctgc tcgtgaggct cgcctaccgc gggaagagat ggtgggctgc gccgggcggt
     1801 gggctcgagt atgacgaaac acacgagacc gccgcgaggc gggaggttgc agaggagaca
     1861 ggcttcgagc tcgatgagct gggaccctgg gcctggaggc gcgaggatgt cttccgcttc
     1921 gaggggcgcc tctaccgcca gcgggagcgc tacttcatag caagcgttcc ggccttcgaa
     1981 cctcacccgc aggtgatcga tgccgaggag gcaaaggcat tcgatgggtt gcgctggtgg
     2041 acactcggag aactagaagc caccgatgaa cggttcgccc ccgccaatct gcccgcgttg
     2101 gtcagagaac tcgtcgagga agggcctccg gaacacccga tcctcgtcag cgtatagctt
     2161 acccgtcgag gatctccgcg ataccgccca ggtcctcgca gtagggttcg acaccttccc
     2221 gatgtttacg cacgagaacg gcgggtatgc cggcggccat ggcgtcgccg ctaagaggct
     2281 gcgacgaaga tcaattcgcg gctcgccacg tcggccggac gactatccca gtccccgaag
     2341 acactctcca ccgagaagcc agcgtcagat agcgaccggc tcaactccgc ccgggtgcga
     2401 aacctcagct cgttgtttga aaccagcact tcaccggagc gcgtgaaaag atagtgaatg
     2461 gcggtgcgca cccgatcgtc ctcgatctcc aggatctcgg tccaccactc gattcgaccc
     2521 gccatggaat cgtcgaccct ccggcgagac gtttgcgccg gccagtccac gtggtctttg
     2581 atcatcttgt tggcccacgg ttggacgagg ggattgcgac tttcgaaagc gatatgaccg
     2641 cccggccgca acgctttgcg gatggcaacg agcgtcgagt gccagccagc atcgtcgagg
     2701 aagaactggg ccacatggcc ggtcataatc gcaagatctg cctggagttc gtccaggccg
     2761 agcacgtccc cctcgatcca ttccacccgt gcgccgcaag cccggcgccg ggccacttcg
     2821 agcatcgctt tcgacggctc gaccccgatc agccggtggc cgcgtttggc caactcgcag
     2881 gtcagcagcc cggtaccaca cccgatgtcg atgatcgacg atgccgagag cttcgacgcg
     2941 agatccagat agaacctttc gtactcggcg acgggattcg ccgtgtcgta gattgcgacg
     3001 agtcggggat cgtagaactc gttgagatcc ggcccgtcgt ccacctcttg atatttgtcc
     3061 tccatgagtt tgaagtctat caacctcgac cctgtctctt gtctttgttc atgcgagtgc
     3121 gcggaaggcc gaccagtaga agatcaagag gtgaggttgg ccaacctcat gcttctagga
     3181 agcccgctct gaaaacacgg cgtaacctcg gtcgaagttt acggcccagg ctaaacggag
     3241 gctgtttggg gagcacctgc catgaagaat attcaccctg gggaccgcgg gccaaggcct
     3301 ttagactagg cgggtggtga gggagactga tctggagctg agagacggtc gcacgctaca
     3361 cgtgtacgac actggttcgg atgacgctga cggccggctc gccgttttct ggcaccacgg
     3421 ctcgccgaac atcggctccc ctccccagcc cctcttcgcg gccgccgagg aactcggcat
     3481 ccgctgggtg tcatttgacc ggccgggata cggtggatca accccccacc cgggtaggaa
     3541 tgtggcatcg gcgtccgctt acgtctcgac catcgccgac gctttaggca tcgatcggtt
     3601 cgcggtcatg ggtcattcta gtggcggttc gcacgccctg gcatgcgcag cactgctcca
     3661 agaacgcgtc cttggcgcgg tcgtagttgc cgggatggct ccgtaccatg ccgagggact
     3721 cgactggttc gaaggattcg ggccaggtgg cgaggcccag ctgcgcgcgg cggcagccgg
     3781 acgcgccgcg ctggagaagc gcctggctga atccgacgac gagccggact tcacccccga
     3841 agacgaagcc gccctggcag gcgaatggtc gtggtttatc gaggtggtcc gtcccgccct
     3901 cgctggcggc atgggagggt tcatcgacga cgacctggcc ggcgtcggag aatggggttt
     3961 cgaccccgcc ggcatcgtcg ctccagtcct tttcttacac gggggacagg atcgagtggt
     4021 gccaagttcg catagcgagt ggctcgcgcg ccgctgcccc acctcagaac tgtggctgta
     4081 cccggaagat gggcacatct ccgtcctcaa cttcagccgg gcggccatgg cttggctcca
     4141 agagcatgcc gatcaaggct gaacctcgcg tggcttcccg gctaggctcg gcctggcggc
     4201 gaacctccct tttgctcgca ctttgcagga ctccgtctcg gccaaccaca cgtcctgttg
     4261 cttcccggtg agatctctta ccatgcccgc gaatcgctga gcaaggttga aggatgcggc
     4321 gatcgcctca tcgtctgctc gaagctgctt cagacagact ttctgctctg cggtgagtcc
     4381 acccacggtc gggctcacga ataacccgag ctcgtagccg gtcagggggc ttcgacgccg
     4441 tcttcggtcg cggtgtccgc cttcggttct cgaagacgta gtgagagcac gaagatggcg
     4501 aggcccgcca cagcgacgac gccgagggcg cccctgaggc ctgccacctc cgccaggccc
     4561 cccacgagcg gcggccccag caggaagccc ccgtagccga aggtggtcac gaccgagatc
     4621 gccgaccccg ccctctcggg caccagctcg ccggctaccg agaaggccac gggcgcgaca
     4681 gcggcgagcc ccatgcccac gagcaggaac ccgccgatga cgagcgcgga gctcgtggtg
     4741 gcgagcgcga gcagcatccc gcccgccgtc agcaggccgg agccgagcag cgtgcggcgg
     4801 ttgcccacgc gcccgacgat ccatcctatg gcgagcctgc ccagcgcctg cgccccgtag
     4861 aagacggcca ctcccgagcc gcctacgatc gcggcgagtc ccagagtgtc gcgcaggtag
     4921 atacccgacc agtgctccat ctcgccctcg gcaagaaggc ccaggcaggc gatcgctgct
     4981 accaacagga gcggaccgct gcggtagaga tcccaccgcc cggcactgcc tttgtctccc
     5041 gagtccgcgg cgggggacgt accgctatgc gatcgttcgt cgcgggtgcc tcgagtcccg
     5101 ggcctcggga accttgccgc cgtgaaggcc gccaccaggg atatcagggg cagcagcagc
     5161 gccaggtaca ccagccggta gtccaccccg gcctgcagca gcgcgcccgc accaaccgcg
     5221 cccgccatcg ccccgccgga gaaggcggcg tgcaggtagg acatgaagcg cctcccgctg
     5281 acacgctcca ggtccaccgc caaggcgttt atccccacgt cgtagaggcc gctgaaggag
     5341 tagaggacca cgagcgtcgc gagcaggacc gtgtaggagc ccgagaaagc aagccccgcg
     5401 atccccatcc cgaaggcgca ggtcgtcagg atcaggtaca ccctccttcc gaggcggtcg
     5461 gaggcccatc cgagggctgc cattgcagct atagacgctc ccgccccgac gaagagggcg
     5521 acgcctagcg gtccgggcga gaggccaagg gcgccgctca ggtccgccag cagcacggcg
     5581 aagcaccccc agaagagccc gaaaaagccg aaggccgaga cctctagcgc cagcaaccgc
     5641 ttccaacgcg tctctgtctg cacgccgccc ctcccgacta gtcggtcctt cgcgcggatg
     5701 gtaggtcgcc gcgtccccgg ctacaagtac gtacccgaaa gtgacatact cacctccggg
     5761 taaccaagga gggaaccacg aggaagatac aaaggaccac gtccactgcg tgctcgaccc
     5821 ggctacgagg acgagcgggc cgtagccttc ctcgacggtt acccgagggc gtatggttac
     5881 accctggtgg ccccgaaaga gcaccgcgag caggtgacgg gtgacttcac gatggaggag
     5941 tacatcgggc tgcagcggct cgtctaccgg gtctcggagg cggtgcgcga ggaggtcggc
     6001 gccgagagga tgtacatctt tacctacggt tccaaccagg gcaactccca cgtccactgg
     6061 cacgtagtac ccttgccgcc cggcgtgcct tacgaggatc aacagggcgc gtggacgagt
     6121 tggagtaagg gcgtgctcga gatcccgcaa agtgagatgg catccctggc cgcatgcatc
     6181 ggacgacggg tcaggggaaa gacgtgaccg actcccaccc gcgccacgta gtggccgtca
     6241 catccgtcgt cgttgagcga gttctggagg tcattctggt gtaaccgatg ctgatcgggg
     6301 gagcacctcg aaggctccga gcgggaactc ctcttcggtg agttcctcga acaggcggtc
     6361 gagcctccga tactcgctgc tgaagcggga gcggaacgtt tcgtatgcgg tacgagagac
     6421 ccagcggtcc agggtgaggt actc
//
LOCUS       DATMVF010000246        16135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_178814, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000246 DATMVF010000000
VERSION     DATMVF010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16135
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178814"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN178_12565"
     CDS             <1..223
                     /locus_tag="VN178_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81846.1"
                     /translation="GGEGGAGSSLGAGGRGGGLQVPVVDLGGGVMARELSTPVGSVLA
                     WSDGGVSYTLAGSVPTAELREAARGLLAR"
     gene            227..1177
                     /locus_tag="VN178_12570"
     CDS             227..1177
                     /locus_tag="VN178_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS81847.1"
                     /translation="MGGRREASVLEVRGFTKKFGDFVAVDSLSFEVRRGDVYGFLGPN
                     GSGKSTTIRAVLGLVEPTEGEIHILGRPAGGHGGREGVAGFVDAPGFYDYLSARDNLR
                     VLAAADRRKGEPPMARVLKTVGLLGRERGKVKTYSTGMKQRLAIASALLREPEVLILD
                     EPTNGLDPGGMRDVRSLIRRLNEDGLTIFLSSHLLAEVEQLCNRVAVIGHGRLLAEGT
                     IAEVVGGDNGRPRYRLVVDDAESALRILEGGAHATGASIADAGPPDGLAGEEIRLSVG
                     PAGAGPVVRDLVAGGVAVLALVPARPSLEDLFLELTEERP"
     gene            1323..2123
                     /locus_tag="VN178_12575"
     CDS             1323..2123
                     /locus_tag="VN178_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS81848.1"
                     /translation="MIRWELYKLVRQRRTLFGLGAMALVPVLFVAALEITREGPPEGE
                     APFATQLLENGLVVPLVTLAFATFVLLPLVVAFVAGDAVAGEVSAGTLKTVLSRSVGR
                     TELFISKAAAVGVYCLAVSAVLAGVGLAAGYLAGGFAPIAGLGGTPISVGRGIALVWA
                     GTGLVSVPLMALAAFAIFLSVTTHNSVGSGVGAVVLVLLLRLASGLGLAGPLDPYLLT
                     NQFEAWLNLTRDPIAWEPIARSLWVSLLWGSFFLAAAWAVFTRRDVLS"
     gene            complement(2274..3185)
                     /locus_tag="VN178_12580"
     CDS             complement(2274..3185)
                     /locus_tag="VN178_12580"
                     /inference="COORDINATES: protein motif:HMM:NF005603.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HWS81849.1"
                     /translation="MEILDASVTEVRLPYPELADSIREVMDGRREGKLDAPSRLVLTL
                     PESGNLLVMPAADDELAITKLVTVHPGNAGHELPTIQGEVVVMEASTGTRLGILDGAA
                     VTARRTAALSLLAARTLAPRPEGPLLIVGAGTQARSHLEAFREGLGVSEVLLTSRTAE
                     RAVSLAEHARSLGMEARVVDGPEEALVAVGLVVTATTSQEPVLPEEIPEGIFVAAVGA
                     FDSEMAELPPRLIGSSIVVVDTIEGVREEAGDLIQAEHAGAFGWEDATQLEDALLWSD
                     KPKGTIVFKSVGHALWDLAAARTAFGS"
     gene            complement(3240..4244)
                     /locus_tag="VN178_12585"
     CDS             complement(3240..4244)
                     /locus_tag="VN178_12585"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWS81850.1"
                     /translation="MRRMVLMVTMAVLVASSLLVSAGFASAAKSKGHVVRPGESIQKA
                     VKAANPGDTIVVSGGVYHGTVTIKKDGISLRGFDAVLKPPAMPSSPCGASGFCVNADN
                     VSISGFTVQNFHDNGIIAIGARNPKIMSNRAINNGEYGIAAFSSTGTHIIGNLTRGSD
                     DAGIYVGDAPRANATVVGNQTYDNLFGIFVRNARHGKIAANRVDDNCLGMLFLADAPG
                     PAGVFDVRGNSVVDNTRACPATKEGPPISGVGIALLGTRGMEIKDNRIIHNVPSGPTE
                     FSGGVVVVSGFEGTPPNNNTLTGNTILRNKPDIFWDKSGSGNDLKPNDCKTSKPGHLC
                     "
     gene            complement(4429..4653)
                     /locus_tag="VN178_12590"
     CDS             complement(4429..4653)
                     /locus_tag="VN178_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81851.1"
                     /translation="MSIAFIGPPGRARAPRPSGSPDPCPRLFSGELVSAEHLLTVVDA
                     SRHMGEVTAEILEALGHPERPEYYASCQRV"
     gene            complement(4976..6481)
                     /locus_tag="VN178_12595"
     CDS             complement(4976..6481)
                     /locus_tag="VN178_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HWS81852.1"
                     /translation="MIEKPRAEETRRYEMLVGGEWVEARSGKTFESVNPYTGRVWATA
                     PEAGEEDVDRAVRAAREAFDEGPWGGMTGTQRARLMRRLAELIAENAESLAVVETTDN
                     GKLLREMGGQMKALPEWYYYFAGAADKIQGDTIPSHNPDFFVYTRREPIGVVGAIVPW
                     NSPLLLLTWKLAPALAAGCTLVAKTAEQTPASTLEFAKLFEEAGFPPGVFNVITGYGT
                     PTGSALVSHPGVDKVAFTGSTETGKLVMKDAADHIAKVSLELGGKSPNIVFEDADLEA
                     AANGVVSGIFAATGQTCMAGSRLFVQEGLHDELVERLAEKARDIKLGNPLETETEMGP
                     IAFKEQLDKVRGYVDAGREEGADVVYGGGSPEDPALKDGFFIQPTILAGVNNEMKVAR
                     EEIFGPVLSVIPASSEEEVVRQANDTPYGLAAAVWTKDVQRAHRVAHALRAGTVWINA
                     YRAVGFTAPFGGYKQSGIGRENGLESVGEYTQTKTVWVELSGKTRDPFTLG"
     gene            complement(6797..7930)
                     /locus_tag="VN178_12600"
     CDS             complement(6797..7930)
                     /locus_tag="VN178_12600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HWS81853.1"
                     /translation="MASILILGGGFGGLAAAHELRARLPDNHEVSVVAADDHFYAGFA
                     KLWDLVGARPLEQGTASLSTLEQHGIRFVQTRITSIDPAERRVETEAGSFGADFLLVA
                     LGAGPGPERILKLQGPAHDLYDANQLPQMRADLAHLDAGTLVVAILGAPYKCPPAPYE
                     AAFLLDEHLRGRGVRDAIELVVTTPQPMTLPAAGSDISQFVAEALEDRDIELRTEQSV
                     QAIDTSTVSFADGGRLEYALLLAVPQAVPPQVVADSPLAGEGGWIQPDRETLLTRFER
                     VYAAGDCTASPPPKAGIFAESEARVAARNIAAEIDGSPGDRFDGTGYCFLEFPGQRAS
                     ALEGHFLAQPPEVRLAEPDTETFVRKQAFETERLRDWLDVGLG"
     gene            complement(8000..8368)
                     /locus_tag="VN178_12605"
     CDS             complement(8000..8368)
                     /locus_tag="VN178_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS81854.1"
                     /translation="MNHDDLQVWLDAYMDAWRRYDPAAIGALFAEDATYAYHPYDEGE
                     EVVRGREAIVADWLEERDEPGTWEAHYHPLVVEGHRAVAEGTTSYANGDLFWNLWTLR
                     FDDDNRCTEFVEWFMTRPHL"
     gene            complement(8575..9087)
                     /locus_tag="VN178_12610"
     CDS             complement(8575..9087)
                     /locus_tag="VN178_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81855.1"
                     /translation="MTVAMRTISGPLEDEERQKALFEGTLLIFKNVEHLATYPITAGR
                     AVALYPQYWGQPLKNTSAGWDLEEVKAGRIAAIVPEPTEPVDTSSELRIVIEPRDHLC
                     FSGTHLHASVPNYTGLARFSIEVRSADVECEADGRGAPNLDGEAPHVAIRWFRRLEDD
                     LPLSEALAGR"
     gene            complement(9148..9783)
                     /locus_tag="VN178_12615"
     CDS             complement(9148..9783)
                     /locus_tag="VN178_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HWS81856.1"
                     /translation="MGDADLLLFFAASVVVILAPGPDNILVLTRGVTLGLKAALVSAS
                     GASTGLVCHSLFAAAGLSAILSRSALAYSVVKYVGAAYLIYLGIRALMDRESFVLSAA
                     DGASPLSLRRVYAQAVASNVFNPKIAVFFLAYLPQFADPTSGGGVAPQLLVLGLAFAA
                     MTWAIFSVVALLSGALGGWLRSKPRFANGLSWLTGGVLIALGLRLALPERR"
     gene            complement(9937..11106)
                     /locus_tag="VN178_12620"
     CDS             complement(9937..11106)
                     /locus_tag="VN178_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWS81857.1"
                     /translation="MTETLRPEDGVRPFRIDIPEQELDDLRDRLGRTRWPDEISGVGW
                     SRGVPLDYVKELAEYWRTSYGWREHEAKLNELPQFTTTIDGQDVHFLHVRSPEPEALP
                     LILTHGWPGSVVEFLDIIGPLTDPRAQGGDPADAFHLVIPSIPGFGFSGPTREVGWTG
                     QRIAAAFAELMRRLGYDRYGAQGGDVGAGVSPDLGRAAPDRVVGVHVNAATIGFMPFP
                     PLDEAELAQLTDSEKARVQRIGEFMAEDFGYARIQSTRPQTLAYGLTDSPVGQLAWIV
                     EKFQEWTHGTERPEDAVDRDRLLTNVMLYWLTGTAGSSGRLYYEDAHSGHWPQPSGVP
                     TGVAVFAEDIAIRRYAEQSNTIVHWSEFDRGGHFAAMEAPDLLIGDVRMFFRGLR"
     gene            complement(11138..11518)
                     /locus_tag="VN178_12625"
     CDS             complement(11138..11518)
                     /locus_tag="VN178_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HWS81858.1"
                     /translation="MDWKLELVAIPVSDVDRAKTFYTEEVGFNADHDHTVSDELRFVQ
                     LTPPGSACSIAIGKGVVDSEPGSAAGLQLVVADINAAHRELVERGVEVSDVQELPGGR
                     FVFFSDPDGNRWSVQEISGSRQNV"
     gene            11672..12313
                     /locus_tag="VN178_12630"
     CDS             11672..12313
                     /locus_tag="VN178_12630"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS81859.1"
                     /translation="MRRENTSGSMSGRRAEAARNDGLILEAAREVFVADPKAPISAVS
                     ERAGVGIGALYRRYGSKEDLLRRLCADGLGIYIAAVEEALADEGDPWKAFTGFMLRVV
                     DADTPSLTLRLAGTFTPTDELYRDSQRAQELNMRLFERTRAAGAIRPDVEVDDIALLL
                     EQLAAVRVGDEERTQQLRHRYLALLLDAIHTPAESRLPGPPPRWEEIDRRWTT"
     gene            12380..12532
                     /locus_tag="VN178_12635"
     CDS             12380..12532
                     /locus_tag="VN178_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81860.1"
                     /translation="MKEEEKIPTVSLHLPARSSVREDYLKELRFPMPGVRNVISGAES
                     GTRIET"
     gene            12588..13541
                     /gene="speB"
                     /locus_tag="VN178_12640"
     CDS             12588..13541
                     /gene="speB"
                     /locus_tag="VN178_12640"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWS81861.1"
                     /translation="MTHYEPADSFETPRFSGVRTFMRLPNVQDLQNADAAIVGAPFDT
                     GATFRVGARFGPEGIRSVSHLLRPYNPSQDVSIFDHISVVDYGDVPVVPGFIEESYER
                     IGVGLKEVHDAGVVPIVLGGDHSIALPELRAAAAVHGPLALVQFDSHADTWDAYFGKK
                     YNHGTVFRRAVEEGLLDPSRSIQVGMRGPLYEAEDLGASRELGFELITTDEIREIGVQ
                     ETAERVRGRVGGAKTYVSFDVDFVDPAYAPGTGTPEIGGFTSREAQEFVRGLTGLEVA
                     GCDVVEVYPQYDGPGQVTALLAANVAYEFLALIAGGKDARG"
     gene            complement(13680..15059)
                     /locus_tag="VN178_12645"
     CDS             complement(13680..15059)
                     /locus_tag="VN178_12645"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HWS81862.1"
                     /translation="MSTVALALGSCTPSPDRRKPGGDYIRYGPLVEDPGGVLDLPEGF
                     QYRIISEEGSALRGGAPIPTRYDGMAAFRGPGNTTVLIRNHELEGGQAPLIGNNPFDS
                     SQAGGTIGIVVGEDRKKVEEFAASCGTVRNCAGGATPWGTWLTCEETEEDDGHGYVFE
                     VMPEDPENDLSRTPIKGMGSFSHEAVGVDPETGVAYLTEDSHSGASFLYRYIPEDPSF
                     RPGSLQRGGKLQALTLEESTLRAKSLEKGRRFGAHWIDVDPGKPHEEAFSKGAVRFKR
                     LEGACFSGGTFWFDDTTGGEERRGQIYRYRPEDETLELFYEGTEANRIESPDNIIVSP
                     WGDLLFVEDEAIIDGDSSNRILGLTPQGELYEFGRCRVGDTELAGPTFSPDGQTFFVN
                     IFKPGMTLAIWGPFMRGSAARQRQMAYAAPPAHLAPELTGELAEAAERFGMSALEAAA
                     YDRLAVPLV"
     gene            complement(15632..15847)
                     /locus_tag="VN178_12650"
     CDS             complement(15632..15847)
                     /locus_tag="VN178_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81863.1"
                     /translation="MIRIRRATASSRTSTVTHIICVLLSLRRSAERVCAGVLVVTVIA
                     SILVAYRREKSAARGGEESRRLQPYAA"
BASE COUNT         2803 a         5211 c         5164 g         2957 t
ORIGIN      
        1 cggtggtgag ggaggagccg gaagttccct gggcgccggg ggccggggag gcgggttgca
       61 ggttccggtc gtggatctcg ggggcggggt catggcgcgc gagctctcca cgcccgtggg
      121 ctcggtactc gcttggagcg acggcggagt ctcttacacg ctcgcgggga gcgtgcctac
      181 cgccgagctc agggaggcgg cgcgcgggct gctggcccgt tagacggtgg gaggtcgtcg
      241 ggaagcttcg gtactggagg ttcggggctt caccaagaag ttcggggact tcgtcgcggt
      301 ggattccctc agcttcgagg tgcgccgggg ggacgtgtac gggttcctcg ggcccaacgg
      361 gagcggtaag agcaccacga tacgggccgt cctcggcctc gtggagccga ccgagggcga
      421 gatacacatc ctggggcgtc cggccggcgg gcacgggggc cgcgagggcg tcgccggctt
      481 cgtcgatgcg cccggcttct acgactacct ctcggcgcgc gacaatctca gggtcctcgc
      541 tgccgccgac cggcgcaagg gggaaccacc catggcgagg gtcctgaaga cggtcggact
      601 tctcgggcga gagcggggca aggtcaagac gtattcgacg gggatgaagc agcgcctggc
      661 gatagcctcc gcgctgttgc gggagcctga ggtcctgatc ctggacgagc cgacgaacgg
      721 cctggacccg ggcggaatgc gcgacgtgcg ctcgctcatc cgccgcctca acgaggacgg
      781 cctgacgatc ttcctctcca gccacctcct cgccgaggtg gagcaactgt gcaatcgggt
      841 ggccgttatc ggtcacggcc gcctcctcgc cgagggcacc atcgccgaag tcgtcggagg
      901 agacaacggc cgcccaagat accggctcgt ggtcgacgac gcagagagcg cgctgcgcat
      961 cctcgaaggc ggcgcccacg ctaccggcgc gtcgatcgcc gacgccgggc cgccggatgg
     1021 gctcgcgggc gaggagataa ggctttcggt cggccctgcg ggagcgggac cggtcgtgcg
     1081 ggacctggtg gccgggggcg tggcagtcct ggcgctcgtg ccggcgcggc cgagcctcga
     1141 agatctcttc ctggagctaa cggaggagcg cccttgatct ccgggccagc tcgcgctcga
     1201 gacgcagcaa ctgtcgaaac ctcctctacg aacctgatac gggtgggttt caaacccgtc
     1261 cctacgggcc ctggtacggg cggttttcaa acccgccccc tacggggagc gaagcgaccc
     1321 ttatgatccg ctgggagctc tacaagctcg tgcgccagcg gcggacgctt ttcgggctcg
     1381 gggctatggc gctcgtaccc gttttgttcg tggctgcgtt ggagatcaca cgcgagggcc
     1441 cgcctgaggg cgaggcaccc ttcgccacgc aactactgga gaacgggctc gtggtgccgc
     1501 tcgtcacgct cgccttcgcc accttcgtct tgttgccgct cgtcgtggcg ttcgtcgcgg
     1561 gggacgcggt ggccggcgaa gtctcggctg gcaccctgaa gacggttctc agccgctccg
     1621 tgggccggac ggagctgttc atctccaagg cggcggcggt gggcgtctac tgtctggccg
     1681 tctcggcggt cctcgccggc gttggcctcg ccgcaggcta cctggcaggc ggcttcgcac
     1741 ccatcgccgg gttgggcggc acccccataa gcgtcgggcg cggcatcgcg ctcgtttggg
     1801 ctggcactgg cctcgtctcc gtacccctga tggcgctcgc cgcgttcgcg atcttcctgt
     1861 ccgtcaccac ccacaactcg gtcggctcgg gcgtgggcgc ggtggttctc gtcttgctcc
     1921 tgcggctcgc ctcaggcctc ggcctcgccg gacccctcga cccctatttg ctcacgaacc
     1981 agttcgaggc ctggctcaac ctcacccgcg accccatagc ctgggaaccg atagcgcgtt
     2041 ccctctgggt cagcctcctc tgggggtcct tcttcctcgc cgctgcctgg gcggtcttta
     2101 cccgccgcga cgtactctct taatgcaggg tgcagaaggt caccccttct gcaccccgcg
     2161 ctggtcagca cggcgcttcg cgccttgtaa gcaactcagc caggacttgg gcaggctaaa
     2221 gctgaaatgc tgagaagtcg cctgttccag gcgacgtgct gacatgcttg tgttcaagac
     2281 ccgaaggccg tgcgggccgc cgcaaggtcc cacagggcgt ggccgacgct cttgaagacg
     2341 atcgttcctt tcggcttgtc ggaccagagg agggcatcct ccagctgggt ggcatcctcc
     2401 caaccgaatg ctcctgcgtg ctcggcctgg atcaagtccc cagcctcctc cctgacgccc
     2461 tctatcgtat ccaccactac gattgagctt ccaatcaatc gtggtggtag ctcggccatc
     2521 tccgagtcga aagcgcccac cgcggcgacg aagatgcctt ccgggatctc ttctggcaag
     2581 accggctcct ggctggtcgt ggcggtgact accaggccca ccgcgacgag cgcctcttct
     2641 ggaccgtcta cgacccgagc ctccatcccc aggctgcgcg cgtgctcagc cagggagacg
     2701 gcccgttcgg cggtgcggga ggtaagcagg acctccgaga cgcccaggcc ttcacggaag
     2761 gcctcgagat gggatcgggc ctgggtcccg gcgcccacga tcaggagcgg tccttcggga
     2821 cgcggggcca gggtccgggc ggcgagcagg gagagcgcgg cggtgcgcct ggcggtgacc
     2881 gcggcgccgt cgaggatgcc gagtcgtgta cccgtactcg cctccatcac gactacctcc
     2941 ccctgtatgg tgggtagttc gtgcccggcg ttccctggat ggaccgttac cagcttggta
     3001 atcgccaact catcgtcggc ggccggcatc accagtaggt ttccactttc cggcagggtc
     3061 aggacgaggc gggagggggc atccagcttc ccctcccgcc tcccgtccat aacttcccgg
     3121 atggagtctg ccagctccgg gtagggcagg cggacttccg tcacgctcgc atcgaggatc
     3181 tccatcgcta ctccgcggcc gtgagccggt cgatagcgag atccagacct acttcacccc
     3241 tagcacaagt gtcccggctt gctcgtcttg cagtcattgg gtttcaagtc attgcctgag
     3301 ccgctcttgt cccagaagat gtccggcttg tttcggagga tcgtgttgcc ggttagggtg
     3361 ttgttgttgg gcggcgttcc ttcgaagccg ctgactacca ccacgccgcc agagaattcg
     3421 gtgggtcctg atggaacgtt gtgaatgatg cggttatctt tgatctccat gcccctggtc
     3481 ccgagcagcg cgatgccgac tccggagatc ggcgggccct ccttagtggc cgggcacgcg
     3541 cgcgtgttat ccactacgct gttaccacgc acgtcgaaca cacccgcagg acccggtgca
     3601 tcggcgagga acaacatccc caggcagttg tcgtcgaccc ggttggcggc gatcttgccg
     3661 tggcgggcat ttcgcacgaa gataccgaac agattatcgt aggtttggtt gccgactacg
     3721 gtcgcgttcg cgcggggggc gtcacccacg tagataccgg cgtcatcgga tccccgcgta
     3781 aggttgccga ttatgtgcgt tcctgtcgag gagaaggcgg cgatcccgta ttccccgttg
     3841 ttgatggcac ggttagacat gatcttgggg ttgcgcgctc cgatcgcgat gatcccgttg
     3901 tcatggaagt tttgcacggt gaagccgctt atggacacgt tgtctgcatt gacgcaaaaa
     3961 cctgatgccc cgcaggggga ggaaggcatt gcaggcggct tgagcacggc gtcgaacccg
     4021 cgcagtgaga ttccgtcctt ctttatggtt accgtcccat ggtagacacc cccggaaacc
     4081 acgatggtgt cgcccggatt ggcggctttg accgccttct ggatcgattc gcccggacgg
     4141 acgacgtgtc ctttggactt cgcagcggag gcgaaacccg cagagacaag cagcgacgag
     4201 gccaccaaca ccgccatcgt gaccatcaag accatgcgcc tcataaacac gacctcccca
     4261 tactagtgcg gcagccggtt gcacaacttg ctccccacgc ctcctatgtg cccactttca
     4321 ccaaaacgtg gttccaagtc tctgcacgcg tgtacaagga aacacattac caaactcgtg
     4381 tgttccagta catacctaaa atggcctatt cttgccgtta cacgccggtc ataccctctg
     4441 gcagctcgcg tagtactcgg gacgttccgg gtgcccgagc gcctccagta tctccgcggt
     4501 aacctccccc atgtgccggc tcgcatccac cacggtgagc agatgctcgg cacttaccag
     4561 ctctcccgag aagagcctgg ggcagggatc tggcgaccca cttggcctgg gtgctcttgc
     4621 ccgcccaggc ggacctatga aggctatgct catgatctac tacctcgtga tcgggtcgct
     4681 ctggtcggaa gtccggtacc cctccaaggg gtgagcccgc gtggggctgg ggaaaggagt
     4741 ggaggtggca ccggaagact attgtcttac cgcatttagt ccgcggaaca actctcaaga
     4801 ggctctcgag gacgaaaatg gcccgaagtg tgcaggacag ttgcacataa gaaggcaata
     4861 gagcagggtt ccggcacgag atacgccgag gtgccactct cttgggcctc cgatgaagcg
     4921 gaggcctcct gtggttccat catggctgct cgcgtttcgg tctgtttcct gatgctcaac
     4981 cgagcgtgaa ggggtcgcgc gtcttaccgg agagctcgac ccagacggtc ttggtctggg
     5041 tgtactcgcc cacggactcc agcccgttct cccggcctat gccgctctgc ttgtagccgc
     5101 cgaacggcgc ggtaaaaccg acggcgcggt aggcgttgat ccagaccgtg ccggccctga
     5161 gggcgtgcgc cacccggtgc gcccgctgga catccttggt ccacacggcc gccgccagcc
     5221 cgtagggcgt gtcgttggcc tgccgcacga cctcctcctc ggaagaggcg gggatcacgg
     5281 agaggacggg gccgaagatc tcctcgcgcg cgaccttcat ctcgttgttg accccggcga
     5341 ggatcgtcgg ctggataaag aagccgtcct tgagcgctgg atcctccgga gacccgccgc
     5401 cgtataccac gtcggccccc tcctcccggc cggcgtccac gtagcctcgc accttgtcga
     5461 gctgctcctt gaaagctatg ggacccatct cggtctcggt ctccagggga ttgcccagct
     5521 tgatgtccct ggccttctct gccaacctct cgaccagctc gtcgtggagg ccctcctgga
     5581 cgaagagcct ggagcccgcc atgcacgtct gtcccgtggc ggcgaagatg ccggagacca
     5641 caccgttggc ggcggcttcc aggtccgcgt cttcgaagac gatgttcggc gacttgcccc
     5701 cgagctccaa ggagaccttg gcgatgtggt cggcggcgtc cttcatcacc agctttcctg
     5761 tctctgtaga accggtgaac gccaccttgt ccaccccagg gtggctgacc agggccgaac
     5821 ccgtgggggt gccgtacccg gtgatgacgt tgaagacgcc cggcgggaac cccgcctcct
     5881 cgaagagctt ggcgaactcc agcgtggaag ccggggtctg ctcggccgtc ttcgccacta
     5941 aggtgcatcc ggcggcgagg gcgggggcca gcttccaggt cagcaagaga agcggcgagt
     6001 tccacggcac gatcgcgccg actaccccga taggctcgcg gcgcgtgtac acgaagaagt
     6061 cggggttgtg ggaggggatg gtgtcgccct ggatcttgtc ggccgcgccc gcaaagtaat
     6121 agtaccattc cggcaaagcc ttcatctggc cgcccatctc ccgaagcagc ttgccgttgt
     6181 cggtggtctc gacgacggcg aggctctcgg cgttctcggc gataagctcg gccaggcgtc
     6241 gcatcaggcg tgcgcgttgc gtaccggtca tcccgcccca aggcccctcg tcgaaggcct
     6301 cacgggcggc gcgtaccgcc cggtccacgt cctcctcgcc ggcctcgggc gcggtggccc
     6361 acaccctgcc ggtgtagggg ttgacgctct cgaaggtctt gcccgagcgg gcctcgaccc
     6421 actcgccgcc cacgagcatt tcgtaccgac gggtctcctc cgcccggggt ttctcgatca
     6481 actgcacctc ttctcctcct tcgtcagccc accataagat cctgccccgt tactacctcg
     6541 gtcccggcgt gctgccagcc ccgctcccag attgcgccgg ggaggggagt ggtcgtagtc
     6601 aggcactacc ctttctcgtg ttgcttactc tcctcccttc cctgaatact tcgccggtac
     6661 acatctggga acgtcacgtc aacgaccgga tgactctcta ctatgaacag caccgcgaga
     6721 agagcccgcg gaggaggccg ggcccgcaac cacactttcc tcctcgtgac ccccgagcag
     6781 ctgtggggta gagacctcag ccgaggccga catcgagcca gtcgcggagc cgctcggtct
     6841 cgaaggcctg tttccggacg aacgtctcgg tgtcgggctc agccagccgt acctcgggcg
     6901 gctgggcgag gaaatggccc tccagggccg aggcacgttg gccggggaac tccaggaagc
     6961 agtagcccgt gccatcgaac cgatcgccgg gactgccgtc gatctctgcc gcgatgttgc
     7021 gtgcggccac gcgggcctcg gattcggcga agattccggc cttcggtggg ggagatgccg
     7081 tgcaatcccc ggccgcgtag acccgctcga agcgggtgag aagggtctcg cggtcgggct
     7141 gtatccagcc tccttccccg gccagcgggc tgtcggcgac gacctgtggt gggaccgcct
     7201 ggggcacggc cagtagcagc gcgtattcga gccgcccacc atcggcgaac gatacggtgc
     7261 tggtgtcgat agcctgcacg ctctgttcgg tgcgtagctc gatgtcccga tcctcgagcg
     7321 cctcggcgac gaactggcta atgtcagatc cggcggctgg cagcgtcatg ggctggggtg
     7381 tggtgaccac cagctcgatg gcgtcgcgga ccccgcgacc tcgcagatgc tcatccagca
     7441 ggaatgcggc ttcgtaagga gccgggggac atttataggg cgcgccgagt atcgccacca
     7501 ccagcgtccc ggcatcgagg tgggcgaggt cggcgcgcat ctggggcagc tggttggcgt
     7561 cgtagaggtc gtgcgccggt ccttgcagct tcaagattcg ctcaggtccc ggacccgcgc
     7621 cgagggcgac caggaggaag tcagcgccga acgagccagc ttcggtctcg actcgacgct
     7681 cggccgggtc gatagatgtg atacgagtct ggacgaaccg gataccgtgc tgctccaaag
     7741 tcgaaaggct cgctgtaccc tgctcgagcg gtcgcgcgcc gacaaggtcc caaagtttgg
     7801 caaaaccggc gtagaagtga tcgtcagcgg ctacaacact cacttcgtgg ttatcgggca
     7861 gtcgcgcccg cagctcgtgg gccgcggcga ggccaccgaa gccaccgcca aggatcagaa
     7921 tgctcgccat gtacatcaac ctcctctaac taccctgggc cttacacccc aactgtacgc
     7981 tctgacgtcc cctcttggcc tacagatggg gtcgggtcat gaaccactct acgaattcgg
     8041 tacatcggtt gtcatcatcg aagcgcaacg tccacaggtt ccagaataag tcgccgttag
     8101 cgtagctcgt tgttccctcg gcgacagccc gatgcccctc gaccactaga ggatggtagt
     8161 gtgcttccca ggtgccgggt tcgtcccgct cctctagcca gtcggcgacg atggcttcgc
     8221 gacctcgcac cacctcctcg ccctcgtcat agggatgata ggcgtaggtg gcgtcctccg
     8281 cgaagagggc gccgatggcc gctggatcgt atctgcgcca agcgtccata taggcgtcga
     8341 gccaaacctg taagtcgtcg tggttcatgt tcgacctccc ggtcagatcg ccggcatacg
     8401 ggccaacgga acgtactcca gcgtgttcgt ctgccctctt tgagagggcg tgcgatcaag
     8461 ccggacagga agccgtgctg gaagatcagc gcgatcatgc cgaatgccgc ggacaagaca
     8521 taatccgggg agtccttggc cagaacgtct cgatggcagt atccgtctac caccttagcg
     8581 gccagccagg gcctcagata agggcaggtc gtcctccagg cgccggaacc agcggatggc
     8641 aacgtgagga gcttccccgt cgagattcgg tgctcctcgt ccatcagcct cgcactcgac
     8701 gtcggcgctg cggacttcga tagagaagcg ggcgagtccg gtatagttgg gaacgcttgc
     8761 gtgcaggtgt gttcccgaga agcataagtg atcccttggc tctattacga tccgcagctc
     8821 cgaggaggta tctactggtt ccgtgggctc ggggacgatg gcggctatcc tgcccgcctt
     8881 gacctcttcg agatcccagc ccgcgctagt gttcttgaga ggttgccccc agtactgcgg
     8941 atagagagcg acggcccgcc cggcggttat ggggtaggtg gccagatgct cgacgttctt
     9001 gaagatcagc agagtgccct caaagagtgc cttctgtctt tcttcatctt cgagcgggcc
     9061 cgagatcgtc cgcatcgcta ctgtcatatt cgtacctcct cggttctcca ggcctgccga
     9121 tttccttact tgcgttcgga tgcttcacta ccggcgctcg ggcaaagcca accgcagacc
     9181 cagggcgatg agcacaccac ccgtaagcca actcaatccg ttggcgaacc gcggcttgct
     9241 cctcagccac ccgcccaatg cgcccgacaa cagtgcgacg acgctgaaga tggcccacgt
     9301 catcgccgcg aaggcgaggc cgaggactag cagttgcggc gcgaccccgc ctcctgacgt
     9361 aggatcagcg aactgcggca aatacgccag gaagaagacc gctatcttgg ggttgaagac
     9421 gttggaggca accgcctgag cgtagacgcg gcgcaaactg agaggtgaag cgccgtctgc
     9481 ggcggagagc acaaagctct cccggtccat gagcgccctt attccgaggt agatcaggta
     9541 tgctgccccg acgtacttca ccaccgagta ggctaacgcc gagcgggaca ggatcgccga
     9601 gagccccgcc gcagcgaaca gggaatggca aacaagcccc gtgctcgcgc cggaggccga
     9661 gacgagcgcg gccttgagcc ctagcgtcac cccgcgggtg aggacgagga tgttgtccgg
     9721 ccccggagcc aagatcacca ccaccgaggc ggcgaagaac aggagcagat cggcatcacc
     9781 cacgatgctc ggcctccttc cctaaattct gatgcggata cgaataaatc ctaacccagg
     9841 gttgtatggc tcgctactga catgggcgtg gaacttggac cacgacgaca tcgcgtaatt
     9901 gatccaaacc ggtgggtttg tgtagtcgtg cggcggtcat cggagcccgc ggaagaacat
     9961 ccgcacgtcg ccgataagga gatccggcgc ctccatggcg gcaaagtgtc ctccgcggtc
    10021 gaactccgac cagtgaacga tagtattgct ctgttcggcg tagcgccgga tggcgatgtc
    10081 ctcggcgaag accgccacgc cggtgggcac cccggatggc tgaggccagt ggccggagtg
    10141 ggcatcctcg tagtagagtc ggccggagga gcccgcggtg ccggtgagcc agtagagcat
    10201 cacgttggtc agcaaacggt ctctgtctac cgcgtcctcg ggtcgttcgg tgccgtgggt
    10261 ccactcctgg aacttctcca cgatccaggc cagctggcct accggcgagt cggtcagccc
    10321 gtaggccagg gtctgcggtc gggtggactg gatccgggcg tagccgaagt cctcggccat
    10381 aaactcgccg atccgctgca cgcgggcctt ctctgagtcg gtgagctggg ccagctcggc
    10441 ttcatccagc ggagggaaag gcatgaaacc gatggtggct gcgttgacgt ggacaccgac
    10501 cacacggtcg ggggctgccc ggcccaagtc cggggagacc ccggcgccaa cgtcgccacc
    10561 ctgggcgccg tagcgatcgt agcccagccg acgcatcagc tcggcgaagg cggccgcgat
    10621 ccgctgcccg gtccacccga cctcgcgggt cggtccggag aacccgaagc ccgggatcga
    10681 cgggatcacc aggtggaagg cgtcggccgg atcgccgccc tgggcgcgag ggtccgtgag
    10741 cggcccgatg atgtccagga actccaccac cgagcccggc cagccgtggg tgaggatcag
    10801 gggcagcgcc tccggctcgg gcgagcggac gtgcaggaag tggacgtcct gcccatcgat
    10861 cgtggtggtg aactgcggaa gctcgttgag cttcgcctca tgctcgcgcc agccgtatga
    10921 ggtgcgccag tactcagcaa gttccttcac gtaatccagc ggcacgccac ggctccagcc
    10981 tacgccggat atctcgtcgg gccagcgggt gcggcccaga cggtcgcgta gatcgtcgag
    11041 ctcttgctct gggatgtcga tccggaacgg tcgaacccca tcctccggtc gtagtgtttc
    11101 ggtcataacc gaaatctcct ctcggcgtgt cgcaggtcta tacgttctga cgcgaaccgg
    11161 atatttcctg cacggaccag cggttgccat ccgggtcgct gaagaagacg aaccgccctc
    11221 cggggagttc ctgaacgtcg ctgacctcca caccccgctc gacgagctcc cgatgcgcgg
    11281 catttatgtc cgcgacgacg agttgaaggc cggccgcgga gccgggctcc gagtcgacga
    11341 ctcctttgcc aatggcgatg gagcaggccg accctggggg cgtcaactgg acgaagcgaa
    11401 gttcgtcgct gacggtgtgg tcgtggtcag cgttgaagcc gacctcttcc gtgtaaaagg
    11461 tcttcgcgcg atccacgtct gagacgggta tcgccaccag ttcgagcttc caatccatta
    11521 tgctcctccc cttcgtactc tggaccgaac gcgatcctgc ccagcgcgcc cgtgtcactc
    11581 gtcctcgaca tgaacaactc cttcctttgg aacaaaccgt tccgctccat tatacttgaa
    11641 cagaatgctc tgttccatat acaattagtg tatgaggaga gaaaatactt ccgggtcgat
    11701 gagtggccgg cgggctgagg cggcgcgcaa cgacggcttg atcctggagg ccgcccgcga
    11761 ggttttcgtc gccgacccga aggcacctat ctctgcggtt tccgagcggg ccggggtggg
    11821 catcggcgcc ctgtaccgcc gctacgggag caaggaggac ctcttgaggc ggctctgtgc
    11881 cgacggtctc gggatctaca ttgccgccgt ggaggaagcc ctggccgacg aaggggaccc
    11941 ctggaaagct ttcactggct tcatgctccg cgtcgtggat gccgacaccc cctcgctgac
    12001 gctgcgcctg gcagggacct tcacgcccac ggatgagctg taccgggaca gccagagggc
    12061 gcaggaactc aacatgcgtt tgttcgagcg caccagggcc gcgggtgcca tccgccccga
    12121 tgtggaagtg gacgacatag ccttgctgct ggaacaacta gcggctgtac gggttgggga
    12181 cgaagagcgc acccaacagc ttcgccaccg atacctggcg ctgctgctcg acgccatcca
    12241 cactccggca gagtctcgat tacccggtcc gccaccgcgc tgggaggaga tcgaccggcg
    12301 ttggacgacg tagccctctc cattatgctc gtttgtacgt ttgcagcatt gcgccgccaa
    12361 gatcgcttcg ggacaataaa tgaaagagga agagaagata ccaaccgtga gtctgcacct
    12421 tccggccagg agtagcgtga gggaggacta cctgaaggag ctgagattcc cgatgcccgg
    12481 cgtcagaaac gtcatatccg gcgcggagtc ggggacacgg atcgagacgt aaggcgatcc
    12541 cagataacgc agccgtaggc aacctctaga gacgggagaa gatgagcgtg acccactacg
    12601 agccggccga ttccttcgaa acaccacgct tctcgggggt aaggaccttc atgcgccttc
    12661 cgaacgtgca ggacctccag aacgccgacg ccgccatcgt gggcgcgccc ttcgataccg
    12721 gggccacttt ccgcgttggt gcccgcttcg gccccgaggg aataaggagc gtctcgcacc
    12781 tcctcaggcc ctacaacccc tcacaggacg tctcgatctt cgaccacatc tccgttgtcg
    12841 actacggcga cgtccccgtc gtgcccggct ttatcgagga gagctacgag aggataggtg
    12901 tgggcctgaa agaggtccat gacgccgggg tggtcccgat agtcctcggt ggcgaccatt
    12961 cgatcgcgct ccccgagctc agggccgcag ccgcagtcca cggcccgctc gccctggtgc
    13021 agttcgactc tcacgcggac acctgggatg cgtatttcgg caagaagtac aaccacggga
    13081 ccgtgttccg gagggcggtg gaagagggat tgctcgaccc ctctcgctcc atccaggtgg
    13141 gtatgcgcgg cccactatac gaggccgaag acctcggggc ttcgagagag ctcggcttcg
    13201 agctgattac cacggacgag atcagggaga tcggcgtaca ggagacggcc gagcgagtcc
    13261 gcgggcgggt cggcggggcg aagacctacg tctcgttcga cgtggatttc gtcgatcccg
    13321 cgtacgcgcc ggggacgggc acgccggaga tcgggggctt caccagccgc gaagcgcagg
    13381 agttcgtccg aggcctcacc ggcctcgagg tggccggctg cgacgtggtc gaggtctacc
    13441 cgcagtacga cggaccgggg caggtaaccg ccctcctggc cgccaacgtg gcgtacgagt
    13501 tcctggcgct gatagcggga ggaaaagatg cccgcgggtg aagagccgcc gcttcgtccg
    13561 agccggcgtg agaatcgagt aagccagaga acatcgtcgg ggcgaagggc tggctcaatc
    13621 cgaagagcga gatcgagttc tgcagagtat cgaggtagtc tggccagaag ccgacgccct
    13681 tataccagcg gcactgcgag gcggtcgtag gctgctgctt ccagcgcgct catcccaaat
    13741 ctctcggcgg cttcggcgag ctcgccggta agctctgggg ccagatgcgc tggtggcgcc
    13801 gcataggcca tctggcgctg gcgagccgcg ctgccgcgca tgaagggtcc ccagatggcg
    13861 agagtcatac ccggtttgaa gatgttcacg aagaaggttt gcccatcggg agagaaggtg
    13921 gggccggcca gttccgtgtc tcccacacgg catctgccga attcgtagag ctcgccctga
    13981 ggggtgaggc cgagaatgcg gttcgaactg tctccgtcta ttattgcttc gtcttcgaca
    14041 aagaggagat ccccccatgg ggagacgatg atgttgtccg ggctctctat ccggtttgcc
    14101 tctgtaccct cgtagaagag ctctagcgtc tcatcctcgg gacggtagcg gtagatctgc
    14161 cctcgcctct cttctcctcc ggtggtgtca tcgaaccaga aggtacctcc ggagaagcac
    14221 gctccctcga gccgcttgaa gcgcacggct cccttggaga aagcttcctc gtgcggcttg
    14281 ccggggtcga cgtcgatcca gtgcgcgccg aagcgccggc ctttctccag gctcttggcc
    14341 ctcagcgtgg actcttctag ggtgagagct tgtagcttgc ctcctctctg caaggagccc
    14401 ggtctgaaac tgggatcctc tggtatgtag cggtagagga agctcgcgcc cgagtgcgaa
    14461 tcctcggtca ggtaggcgac gccggtttcg gggtccacac caaccgcctc atgcgagaac
    14521 gagcccatcc cctttatcgg ggtcctggag aggtcgttct cgggatcctc gggcatgacc
    14581 tcgaagacat agccgtgccc atcgtcctcc tcggtctctt cgcaggtaag ccacgtcccc
    14641 catggagtcg ctcctccggc gcagttgcgg acggttccac aggaggctgc gaactcttcc
    14701 accttcttcc tatcctctcc taccacgatt ccgatcgtcc ctccggcctg ggaggagtca
    14761 aatggattgt tgccgatgag aggggcctgg cctccttcta gctcgtggtt acgtatcaga
    14821 acggtggtgt tgccaggccc ccgaaaggcg gccatgccgt cataacgagt ggggatcggt
    14881 gctccacccc tcagagcgga gccctcttcg gagattatgc ggtactgaaa tccttccggg
    14941 agatccagga ctccgccggg gtcctcgacc agcggaccgt agcggatgta atccccgcca
    15001 ggcttcctcc tgtccggcga tggcgtgcag gaaccaaggg cgagggcaac ggtgctcatg
    15061 gctcctagcc tcaggaaagc ccgacggctc atggggggcc aataccccct acctctagag
    15121 atcccaggat tcctctcgct atccaccgct aagtgacccc tctgctacag tgaagtgcgc
    15181 cgcagcatgc ccgaaatgcg cgccaacagc accaggtccg gccagaccgg cgttctccaa
    15241 caccgcgtcc acgttgtcta cacatccggt tggaacctac ttcacccttg gttggaatgg
    15301 ggatcgacct tttaagattc tatgatcccg gtagccaaac tgaaagggct cgaacgcaaa
    15361 atttagcgta gaacgcagga cgctggggat gactcggtct tcgtcaccat cctcgcagac
    15421 gaagaaacat taggtgggcg tcccggccga cctccagaag ccccaggcga gcccgaagga
    15481 ttgcgaggcg tctcccgtgg ccgtcgagga gtctgcttga ccgtaccgga cggggtttga
    15541 tcccctgcgg cgcgcgatag tcgggctgca ggaatctcca cccccggctc cacctttcgg
    15601 ccgatgccgc tacacccgac cggccgctac attaggcagc gtagggttgg agccggcggc
    15661 tctcttcccc tccgcgagcc gccgacttct cccggcggta ggcaaccagg atcgaggcga
    15721 tgaccgtgac gacaagaacg cctgcgcaca cgcgctctgc gctccggcga agggagagga
    15781 gcacgcagat gatatgtgtg accgtcgagg ttcgtgagga cgccgttgcg cgccggatgc
    15841 ggatcatcgc cccctccatc gaggggccat gaagatggcg ggaggaggga agcccggccg
    15901 cagggcgcga ctgctcttcc cgatagaccc cgaggccttc ttcgtccccg aggcccgcga
    15961 ccggcgagag gcagcgtgag atgcgaccga gcacaccgcg cggccaaggc gattcccgca
    16021 cccgggaaac gtcagagagg atttgcccta ctggatcacg ttgaagcatc acggcaacgg
    16081 accggcaacc gataaaggag acagcgatga gagttccatc ccgcaccgcg tccgg
//
LOCUS       DATMVF010000247         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_537436, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000247 DATMVF010000000
VERSION     DATMVF010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537436"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VN178_12655"
     CDS             complement(<1..768)
                     /locus_tag="VN178_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81864.1"
                     /translation="MVVLLLLLSDALLAMLVWLAAYEVQSLWGRLTVIGWEGFPPQTA
                     LVVGVFAVAVWIGLRSLMGLYPGYGLSSAERLRRHTYSVVGAFAAVSFAIALDFRDIA
                     PKFKLFDLYGNFPRLILVLGFVGLLLLSPLVQSLARWGVWRLGIWGRPVVVIGDKETS
                     ARTVKLLEEEWVLGFAPAALIDRGLTLSGKSLNSRSHEEAMTQAENLARGRGVDTIIL
                     ATPFIRRSHLAPLVSRASRTFRHVLLISHLGGLTNSAV"
     gene            complement(1313..2590)
                     /locus_tag="VN178_12660"
     CDS             complement(1313..2590)
                     /locus_tag="VN178_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWS81865.1"
                     /translation="MADVTDEGASRTQAPSTLGRRLRYAGPGIVIAVTGVGAGDMVSS
                     LVAGTDFGMVLLWAVVLGAILKYFLTEGIGRWYMASGQTILQGWRSLGRWAIWYFVIY
                     LFIVTFVFGAAVTSTAALAVDAAFPGVLPLWAWAALHSVAAFVVVGVGRYGLFENIMK
                     TFAALKFGIVILLAILLAPSLGEIALGFVPRIPEGALINVLAIIGGVGGTYSLAAYTY
                     WVRERGWRHSDWIPTMRTDLTFGYALTALFMVSMLIIGAELLFGTGASISDEEGLLAL
                     VDPMQDRFGLLARWAFLIGFWAVATGAMLGAWNGGAHLFADCVRTIREVPDEEASDYL
                     SEKSIYFRGFLAWMSFPPMVLLAFGQPVLLVIVYASLGALFLPFLAVTLLILLNSERV
                     APEYRNRLVSNVVLAVSVIPFIVVGVQEVLGSL"
     gene            complement(2632..2997)
                     /locus_tag="VN178_12665"
     CDS             complement(2632..2997)
                     /locus_tag="VN178_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWS81866.1"
                     /translation="MPKLEVEGVGVFEVPENKRLVLAIEEDAGVDILHRCGSYARCTT
                     CRIEYLEGEPEKMTRAELEVLEKRNLLGQVRLSCQTLADHDKKVRVLMTVSSTGLDGP
                     GPHPEPQITPEPEWVDKPA"
     gene            3150..3512
                     /locus_tag="VN178_12670"
     CDS             3150..3512
                     /locus_tag="VN178_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HWS81867.1"
                     /translation="MDHRPPSIKVDKPWGRFEQYTHNLPSTVKIITVAPGGTLSRQYH
                     HRRDELWVILDKGAQVELGEEVLEPESGEKVFIPRGTVHRLSTAGQSEVRILEISFGE
                     FDEEDIVRLDDVYGRVVQ"
     gene            3534..>4374
                     /locus_tag="VN178_12675"
     CDS             3534..>4374
                     /locus_tag="VN178_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HWS81868.1"
                     /translation="MGLRGEEEPGRAEHAQTGARRDRRRPTPLLLLFALLLLALLAVA
                     AILLWRSLGESISGTSIARDSIDSAGPEPRVRLANAAGRVRVEGVEGSGSVDYEARRY
                     AMAADPASAKQQASEVPVDISREDSMIVIETDGGENTGADYALRVPTGGSVEVESEVG
                     DVEVSGISGNVTVSAEAGDVRVRDLGGDVEVQAPRGDISVADVNTDTGGANLEVGVGD
                     VSLEDLILGTLEASVEAGDVNLSGRFSGGGRITVETGDVIVRLPPDDTRDLTLETRVG
                     SVLR"
BASE COUNT          899 a         1387 c         1318 g          770 t
ORIGIN      
        1 taccgccgag ttggtcaatc cgccgaggtg cgagattagc agaacgtgcc ggaaggtgcg
       61 gctagcccgg ctgacgagcg gtgccaaatg cgagcgccgg ataaagggcg tggcgaggat
      121 tatggtgtcc acgccgcgtc cgcgagcgag gttctcggcc tgggtcatgg cctcttcgtg
      181 ggagcggctg ttgagcgact tccccgacag cgtcaggccc cggtcgatga gggcggccgg
      241 cgcgaatccc agcacccatt cctcttccaa aagcttgacc gttcgggcgc tcgtttcctt
      301 atctcctatg acgaccacgg gcctgcccca aatgccgagc ctccacaccc cccaccgcgc
      361 tagactctgt accagggggg atagaagcaa aaggcccaca aagcccagta ccaatatcag
      421 gcgtgggaag ttcccgtaaa ggtcgaagag cttgaacttc ggggctatgt ccctgaagtc
      481 caaggctatg gcaaaggaaa cagcggcgaa ggcgccgacc acggagtacg tgtgccggcg
      541 aagcctctcc gccgagctca gaccataccc cggatacagc cccatcaatg aacgtaaccc
      601 tatccacacc gccacggcga aaaccccgac caccagtgcc gtctgcggag ggaagccctc
      661 ccaccctatc acggtcaacc ggccccagag gctctgtacc tcgtacgccg ccagccagac
      721 cagcatcgcc agcaaggcgt ccgacaataa caacagcagg acgaccaccg gttgcctcca
      781 agcccgcgcc aggcggcgtt tcggcagccg cgcgtgctgc gatgggggct cttctatctt
      841 ttgagcggta ttgtctatgc tgggaacctg actcctcctc aacatcctct ccccctcaac
      901 cgagcacgtt gctacttgtc cggagtccta tgcaaatcgc cgataaacgc tgaagcacga
      961 atcttccaga gaatcaacgt agccatcatc gttttcagac ggccagactt cgcttgagtt
     1021 gttcggctgt ccctctcttc atccccctcc tgatctttgg cgaagctact acatctctgt
     1081 gaatccttta ggccgtagga cctcggccca taggccggta tccgttatac gtagcagttc
     1141 tcggattgct tcaacagcct ccaatggttt tctgatacgc acactactcg tctattcgat
     1201 cagcacctta ctatatcgca acataaacca atcggataca cctatttgtt aatcagatca
     1261 gttactttgg ctgttgcggg tggcgcaatt tctattacga gcggtcttgc ggttacaggc
     1321 taccgagcac ctcctgcaca cccacgacga tgaaaggaat caccgagacg gcgaggacca
     1381 cgttggagac gagccggttg cggtactcgg gggccacgcg ttcggagttc aggaggatga
     1441 gcagggtaac tgccaggaac ggcaggaaaa gagcaccgag tgaggcgtag acgatcacca
     1501 gcaggaccgg ctgaccgaag gcgagcagga ccatgggcgg gaacgacatc caggccagaa
     1561 atccccggaa gtagatgctc ttttcggaga ggtaatcgga tgcctcttcg tctggcacct
     1621 cgcggatagt gcgcacgcag tctgcgaaca ggtgtgcgcc gccgttccag gctccgagca
     1681 tggcaccggt cgcgaccgcc cagaagccga tgaggaatgc ccagcgtgcc aggagaccga
     1741 atcgatcctg catggggtct accagggcca gcagaccctc ctcatcgctt atggacgcgc
     1801 cggtaccgaa gagcaactca gccccgataa tcagcatcga caccatgaac agggccgtta
     1861 gcgcgtagcc gaaggtcagg tcggtccgca tggtcgggat ccagtccgag tgccgccagc
     1921 cgcgctcgcg cacccagtat gtgtaggcgg ccaacgagta ggttccgccc acgccgccga
     1981 tgatggcgag gacgttgatc agggcgccct ccggtatcct cgggacgaac cccagggcga
     2041 tctcgccgag ggacggggcg agcaggatgg caagcagaat gacgatcccg aacttcaggg
     2101 cggcgaaggt cttcatgata ttctcgaaca atccgtagcg ccctacgcct acgacgacga
     2161 aagccgccac gctgtggagc gcggcccagg cccacagcgg cagtacgccg gggaacgccg
     2221 catccactgc gagcgcggcg gtcgaagtta ccgcggcccc gaagacgaac gtcacgatga
     2281 acaggtagat cacgaagtac cagatcgccc accggccaag cgaacgccag ccctgcaaaa
     2341 tggtctgccc ggaggccatg taccaacgcc ctataccctc ggtcaggaag tacttgagga
     2401 tcgcaccgag aactacggcc cacaggagga ccattccaaa gtccgtcccg gccaccaggg
     2461 aggagaccat gtccccggcc cccactccgg tcaccgcgat gactatgcct ggtcccgcgt
     2521 acctcaaccg gcgtcctaga gttgatggag cctgcgtcct cgaagcaccc tcatcggtta
     2581 cgtcagccat ctgtcccttc ccccacactc ctcaatagac accattgttt ctcaggccgg
     2641 cttatccacc cactcgggtt cgggtgtgat ttgcggctcg ggatgtggac ccggaccgtc
     2701 gaggccggtg gagctaacgg tcatcaatac ccgcaccttc ttgtcgtggt cggccagagt
     2761 ctggcacgag agccttacct gcccgaggag attgcgcttc tctagcactt cgagttcggc
     2821 cctggtcatt ttctcgggct cgccctctag atactcgata cggcaggtgg tgcacctggc
     2881 gtaagaacca cagcggtgca ggatgtctac cccggcatcc tcttcgatcg ccaaaaccaa
     2941 cctcttgttc tcgggcactt cgaaaacccc cacgccctcg acttccagct tcggcataca
     3001 tccctccttc cagagacgaa gcttccgtgc ttgtatctaa ccacgccggc agaccaggcg
     3061 cgaatgccgg gtcgcgggct ttcggggtta tacgcctcca aaaccgcggg tggatcggct
     3121 aaactggccc ttcacgaaag gggtcacgca tggatcaccg tccgccttcc ataaaggtag
     3181 acaagccttg gggcaggttc gagcagtaca cgcacaacct gcccagtacg gtcaagataa
     3241 tcaccgtcgc tcctggcggc acgctctcgc gccagtacca ccaccgccgc gacgagctct
     3301 gggtcatact ggacaaggga gcccaggtag agctcggcga agaagtgctc gagcccgaga
     3361 gtggggagaa ggtattcatc ccccggggga cggtgcacag gctctccacg gccggccaaa
     3421 gcgaggtgcg gatattggag atctcctttg gggagttcga tgaggaggac atcgtcaggc
     3481 tcgatgacgt ctacggccgt gtagtgcaat gacgattctg gattagatcc aggttgggct
     3541 taaggggcga agaggagcca gggcgcgccg agcatgcgca gaccggagcc cgcagagacc
     3601 gccggcggcc tactccgctg ctcctgctgt tcgccttgct gctccttgcg ttgctcgcgg
     3661 tggccgcgat cctcctctgg aggtccctcg gcgagagcat ctcgggcacc agcatcgcca
     3721 gggattccat agattcggca ggtcccgaac cgcgcgtacg gctcgccaac gccgcgggcc
     3781 gggtgcgtgt ggagggcgta gagggatccg ggtccgtaga ttacgaggcc aggcgatacg
     3841 cgatggccgc cgaccccgcc tcggccaagc agcaggcctc cgaagttccc gtggacatct
     3901 cccgcgagga ctcgatgatc gttatcgaga cggacggggg agagaacacg ggcgccgact
     3961 acgcgctcag ggtaccgacc ggtggctcgg tcgaggtcga atccgaggtc ggtgacgtcg
     4021 aggtctcggg tatctcgggc aacgtgacgg tcagcgccga agccggtgac gtgagggtca
     4081 gggacctcgg tggcgacgtc gaggtccaag cacccagggg agatatcagc gtcgccgacg
     4141 tcaacacgga caccggaggt gcgaatctcg aggtcggcgt gggggatgtc tcgctcgaag
     4201 acctgatcct cggcaccctg gaagccagcg tcgaggccgg ggacgtcaac ctctccgggc
     4261 ggttctccgg cggcggacgg atcaccgtgg agaccggcga cgttatcgtc aggcttcctc
     4321 cggatgatac gagggacctt acgctcgaga cgcgtgtcgg aagcgtcttg cgcg
//
LOCUS       DATMVF010000248         4794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_675863, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000248 DATMVF010000000
VERSION     DATMVF010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675863"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VN178_12680"
     CDS             <1..298
                     /locus_tag="VN178_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81869.1"
                     /translation="LGPILKGRRVIVEGRLPGAVRYALIRLGAEVEEQRPKNEERYVD
                     MTPVIFVFVALAMAARVVSLGLDDQTMYILSGIGFAVLYGLRPFISKLQRPRDD"
     gene            complement(418..1584)
                     /locus_tag="VN178_12685"
     CDS             complement(418..1584)
                     /locus_tag="VN178_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWS81870.1"
                     /translation="MNEGESEERIVSPQRRLELLAGVPAFSHLPAPVLEELASRLTEE
                     RFRPSDMVVVEGDTDDRVYLIVEGRAEASTTGPSGIVPLAALGTGELFGELSLLEAGS
                     SRQATITAVEPLLLLGLRAADFRLALGAHPESRSAFERLADDLLVTKLLKQASPFSTL
                     DGERLRRLAARLERLEVSTGETIIRQGEAGEECYVLRSGRVEVLVRGAQGDERVLATL
                     DPGSLFGEAALLTDGPRNATVRATEPCTLLALRRTDLLEVLDEDRQTRERMLELVRMR
                     DRPRRVRGIEVHHRTTITGETITTLKDARRDTNYRLSPAGWFVWQRLDGEHTLRDLTS
                     EYLATHEASASHAVVEAVVGLVAAGFVEGVKPSPEEFAEEPTFWQRVRAAIRRF"
     gene            1795..2937
                     /locus_tag="VN178_12690"
     CDS             1795..2937
                     /locus_tag="VN178_12690"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWS81871.1"
                     /translation="MQKRASKNSAIARGNHDVLGPRELNRATLERQMLLRRRELPALD
                     AIEHLVGMQAQAPSPPYVGLWTRLEGFHPGELGRLITERRAVRIALMRNTVHLVSARD
                     CLALRPLMQPVLNRTLYSTRANRAHLDGMDIEALVAAGRALLEEKPRTANELGKLLQE
                     RWPERDPASLARAIRHLLPLVQVPPRGVWGESGPAAHTTAESWLGRPLDPSPSIEDIV
                     LRYLGAFGPATVKDVQTWSGLTRLGEAVERLRPRLRMFSDESGKELFDVPDAPMPDPD
                     APAPPRFLPEFDNLILSHADRTRFIAGEYRKALTSRYGMVPASVLVDGFVRGTWKTER
                     SRGMATLEIKPFEPLAKEHRAALVEEGERLVRFTGAENFEIRFAGP"
     gene            complement(3070..3984)
                     /locus_tag="VN178_12695"
     CDS             complement(3070..3984)
                     /locus_tag="VN178_12695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HWS81872.1"
                     /translation="MPVETLEAVVERHGLSVGEISPSKDAGIFNAIYLVGNEYVLRVP
                     RDAPPFVAAICKESVAVPAAREAGVRTPALVAFDDTLEILPVPYAIYERVHGETLGAL
                     DLGPEDTPRVWQRVGGDLARLHERVGEYGPIADLECEPLPDPRPMPDELVSAGYFTAE
                     EARWLSEWLELLAPFALAPLPERFRHGDLQTTNVMVRPGLPTYLALIDWGACGWGDAA
                     HDFAGIPLRAVPFMLEGYREVAPLPEDETAEARILWRHLQIALYLLGRPPQPGLSWAE
                     RPAAMLLEIMRFFLGSPGRPWMDLYLAP"
     gene            4132..4497
                     /locus_tag="VN178_12700"
     CDS             4132..4497
                     /locus_tag="VN178_12700"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS81873.1"
                     /translation="MKIGSIVIRCYEFDRTLAFWREALHYLPREPVDDGWVVLCDPAG
                     RGPNMSLEKIPEPFEPIGRTSRVHLDLYTDDQQGEVERLIGIGASRYPRTYSPDEDFI
                     VLEDPDGNRFCVVQKTSER"
BASE COUNT          886 a         1579 c         1467 g          862 t
ORIGIN      
        1 cctcggcccg atcctcaagg gcaggcgcgt tatcgtcgag ggccgactcc ccggcgcggt
       61 acgatacgcc ctcattcgcc tcggtgccga ggtcgaggaa cagaggccca agaacgagga
      121 acgctacgta gacatgacgc cggtgatctt cgtcttcgtc gccctggcta tggctgcgcg
      181 tgtcgtaagc ctcggcctcg acgatcagac gatgtacatc ctatccggca tcggcttcgc
      241 agtgttgtat ggcttacgtc cctttatctc caagttgcaa cgtcccagag acgattgaag
      301 gtagcgcggc gccaaactcg accgcgacac gtcggagccg caaatgctgc tgcgtcgcct
      361 agaacggcaa aggccacttc gatgtgcccg acgcgttcga acccggtgct tgctgctcta
      421 gaagcgccgt atcgctgccc gtacccgctg ccaaaaggta ggctcctctg cgaactcttc
      481 ggggctcggc ttcactccct ccacgaaacc tgccgccact agtccgacca ccgcctccac
      541 tacggcatgc gacgcggacg cttcgtgcgt ggccaggtac tcggatgtga gatctcgcaa
      601 ggtgtgctcc ccgtcgagac gttgccaaac gaaccagccg gcgggcgaga ggcggtagtt
      661 ggtatcgcgc ctggcgtcct tgagggtggt gatggtctct ccggtaatag tggtgcgatg
      721 gtgcacctct atcccgcgca cccgacgcgg gcgatctcgc atccgcacga gctcgagcat
      781 acgctctcgt gtttgccgat cctcatcgag gacctcgagt agatccgtcc ggcgcagcgc
      841 gagcagcgtg cacggctcgg tggctctcac cgtggcgttg cgtggtccgt cggtgagcag
      901 ggccgcctcc ccgaagaggg agccgggatc taacgtggcc agaacgcgtt cgtcgccctg
      961 tgctcccctc acgagcacct ccacacgacc gcttcggagc acgtagcact cttcgccggc
     1021 ttcgccctgg cgaatgatgg tctctcccgt ggagacctcc agacgctcca gccgtgctgc
     1081 gaggcgccgc agccgctcgc cgtccagcgt ggagaagggg ctcgcttgct tgagcaactt
     1141 cgttaccaaa aggtcgtccg ccagcctctc gaaggctgag cggctttcgg gatgtgcgcc
     1201 gagcgccagg cggaaatctg cagcgcgcag gccaagcaag aggaggggct cgacggcggt
     1261 gatcgtggct tgtcgactgc ttccggcctc tagtagggac agttctccga acagttcccc
     1321 ggtccccaat gccgccagag gaacgatacc cgagggtccg gtcgtcgatg cctcggcccg
     1381 accttccacg atcaaatata cccgatcgtc cgtgtcgccc tcaaccacta ccatatcgga
     1441 aggccggaag cgttcctccg tcaggcgtga agccagctcc tccagcaccg gagcgggcag
     1501 gtgcgagaaa gccggtacgc ctgccagcaa ctccaggcgc ctctgcggtg agacgatccg
     1561 ttcctccgat tccccttcat tcacggccac aaacctttcc gagcctgcga cgtttcttgc
     1621 tcgagatcat taaacaccag cctgtgtccg gatcaagacg tagcgggtcg tgccggactt
     1681 cggtgaggag catgcaaacg ccacgcagga cgcattgcgg tagattgtgg tgtggacgga
     1741 cagacccgtt ccctacagcg tacccgggtc tcggagtccg cggaaaggag tagcatgcag
     1801 aagcgagcgt cgaagaactc cgcaatcgcg cgaggcaatc atgatgtgct cggtccgcgc
     1861 gaactcaacc gcgccacgtt ggagcggcaa atgctgctgc ggcgccgaga gctgcccgcc
     1921 ctcgatgcca tagagcacct ggtcggcatg caggcacagg ccccctctcc gccctacgtt
     1981 ggattgtgga cgcgtctgga gggtttccat cccggcgagc tggggcgcct gatcaccgag
     2041 cggcgcgcgg tgcgcatcgc gctgatgcgc aacacggtgc acctggtcag cgcccgagac
     2101 tgcctcgcgc ttcgcccact gatgcaaccg gttctgaacc gcaccctgta ctccactcgc
     2161 gccaaccgcg ctcacctcga cgggatggac atcgaagcgc tcgtcgcggc tggccgcgct
     2221 ctgctcgaag agaagccacg caccgctaat gagctgggga agctgctaca agagcgttgg
     2281 ccagagcgcg accctgcctc cctcgcccgc gccattcgcc acctcctgcc gctggtccag
     2341 gtccctcccc ggggtgtctg gggagagagc ggcccagccg cccacaccac ggccgagtcc
     2401 tggctcggcc gtccgctgga cccatcccct tccatcgaag atatcgtgtt gcgttacctc
     2461 ggcgctttcg gtccggccac cgtcaaggac gtacagacct ggtcgggcct gaccaggctc
     2521 ggcgaggcgg tcgagcgtct ccggccgcgc ctgcgtatgt tcagtgacga gagcggaaaa
     2581 gaactcttcg atgtgcccga cgctccgatg cccgaccctg acgcccctgc tccgccgcgc
     2641 ttcttgcccg agttcgacaa cttgatccta tcccacgcag accgcacccg cttcatcgcc
     2701 ggggagtacc gcaaggcact cacctcgagg tacggcatgg tcccggcctc cgtgctcgtc
     2761 gacggctttg tccgcgggac gtggaagacc gagcggagcc gtggtatggc taccctggag
     2821 atcaaacctt tcgagcccct ggcaaaggag catcgcgccg ccctggtcga ggagggagag
     2881 cggctcgtcc gttttacagg cgccgagaat ttcgagatcc gcttcgccgg gccctgaaga
     2941 acatcgacag cgtccagggc aggtttactc gcccgactct tgcccgtagc tgttggcggc
     3001 gcgaacatcc ctccaggctg gtagtcgaat tagtcttggg ggcaagccgg aatccgcgat
     3061 gctcgtcgtt cacggggcca ggtacagatc catccacggc ctaccaggcg aaccgaggaa
     3121 gaagcgcatg atctcgagca gcatcgccgc cgggcgctcg gcccacgata gtcccggttg
     3181 tgggggccgt ccgagaagat aaagcgcgat ctgcaggtgc ctccacagta tcctagcctc
     3241 cgccgtctcg tcttccggaa ggggagccac ctcccggtat ccctcgagca tgaaagggac
     3301 cgccctgagc ggtatcccgg caaaatcatg ggccgcatct ccccagccac acgctcccca
     3361 gtcgatcaaa gcgagatacg ttggcaggcc gggacggacc atcacattgg tcgtctgcaa
     3421 atctccatgg cggaaacgct ctggtagcgg ggcgagcgcg aagggcgcca gaagttccaa
     3481 ccactctgaa agccagcggg cctcctcggc ggtgaagtat ccggccgaga ccaattcgtc
     3541 cggcatcggc ctcgggtcag gcagcggctc gcactcgaga tccgctatcg gtccgtactc
     3601 gcccactctc tcgtgcagtc tggcgaggtc gccccccact ctctgccaca ccctcggggt
     3661 atcttcggga ccgaggtcaa gcgcaccaag cgtctctccg tgtacccgct cgtagatggc
     3721 ataaggcaca ggaaggatct ccagggtgtc gtcgaacgct acgagcgccg gcgtccttac
     3781 cccggcctcg cgggctgcgg gcacggcaac cgactccttg cagatggcgg cgacgaacgg
     3841 aggcgcgtcc ctgggcacgc gcaggacata ctcgtttccg accagataga tcgcgttgaa
     3901 gatgccggcg tctttcgacg gcgagatctc gccgacgctc agaccgtgcc gctcgacgac
     3961 ggcctccagg gtttcgaccg gcatcggcgg aaaatccggt agctgcaaag tagatgcctc
     4021 tcgtgtccga gttcagaggg tgctgaatat acatgaggtt ggcaacgggc tcccgtacgg
     4081 gccatcatga tttacctggt tcgaatgttg gctttcctcg aaggaggaac gatgaaaatc
     4141 ggctcgatcg ttatccggtg ttacgagttc gacaggacgc ttgcgttttg gcgagaagca
     4201 ctccactacc ttcccagaga acctgtcgat gacggctggg tggtactttg cgatcctgcg
     4261 ggaagagggc ccaatatgtc tttggagaag attccggaac ctttcgagcc catcggcaga
     4321 acgagcagag tgcacctgga tctctacaca gacgaccagc agggcgaagt ggagaggttg
     4381 atcgggatcg gggcgtcccg gtacccacgc acttacagtc cggatgaaga tttcatcgtg
     4441 ttggaagatc ccgacggcaa ccgcttctgc gtcgtacaga aaacaagcga gaggtgaacg
     4501 atcctcccga caaacccggc cttagccggc gcccggaaaa caccggtaca gcaaggtcag
     4561 accatcggcc catgatggta tgtggaactt gcggtcttcc catatgtacc tgtcgacgca
     4621 ggcagtcagc gatccagtca gcccttcaag cggccaagat gtccgaatcg tcacgcgggg
     4681 gaggcgtgcc tgcgggcgag gtacttacgc accctctcgc ggttgccgca gtcgcgcgag
     4741 tcgcaccacc ggcggctgcc gttgcggctc tcgtccagga acatccagcc gcag
//
LOCUS       DATMVF010000249         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_27483, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000249 DATMVF010000000
VERSION     DATMVF010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27483"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..924)
                     /locus_tag="VN178_12705"
     CDS             complement(16..924)
                     /locus_tag="VN178_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS81874.1"
                     /translation="MEIDGFRGRLISADHADYDIARAVWNRAIDRRPRLIARCIGSAD
                     VVAAVRFARDHDLEIAIRGGGHNVAGTAVCDDGIVIDLSAMRAVRVDPADRRAWVQGG
                     ALWADVDHETQAHGLATTGGIVSHTGVAGLTLGGGIGWLAGRYGLACDNLTSANVVLA
                     DGRVVTASPTANDDLFWGLRGGSGNFGIVTSFEYQLHPVAGVLAGPVFHPFSAAAAVF
                     GFYRDFVAAVPDELVTLAGFLHTPEGVPVAGVIGCWNGPFDAGERAVQPMRTFGAPLV
                     DEIQPMPYAAIQAALDPALPPGAATT"
     gene            1056..1772
                     /locus_tag="VN178_12710"
     CDS             1056..1772
                     /locus_tag="VN178_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS81875.1"
                     /translation="MRNTRVVRTYGQYCPIARGAEIFAERWTPLIIRNLYLGCGSFSE
                     ILEGAPGLSRTLLSQRLKQLERVGIVESAPKPDGRGHHYKLTSAGHELFTVCQSLGEW
                     GARWLEIAPENLDPFVALWSMCNALRRDRLPDRRVVIRFDFTGRPRRERYWLLIELGD
                     TEICKKYPGLDEDLYITAEAEAFVKWHAGQLTWAQATREGRIQLDGPSSLVRAFPTWN
                     ARSMFAHIRPVSRTLTSPAG"
     gene            complement(1943..3694)
                     /locus_tag="VN178_12715"
     CDS             complement(1943..3694)
                     /locus_tag="VN178_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS81876.1"
                     /translation="MGKKRRHRVEPTDDWEQLKPLCGWPEQVSYEEIRPLVLFGSSVT
                     QRTEQTGTAQRTLYRRVKRFEAEGMDSLFSTERAKRFGLPPAIRRFIVDLKAEHPPMR
                     PNEIASVCYVRFGRHPDGRTVARVLQEEPIPLRMVRRYPPYHEIPEAKERRMAIVRLH
                     AEGWKVKSIASYLKTYRSTVYRALRRFIEEGLEGLEDKPKKEGAGGGVRKVDLKAIAA
                     VRRLQQNPELGESRIHGALKRMGIHLSPRTCGRVLALNRRLYALDKPKRGSHQKRRMP
                     FASSRRREYWSADVRYVDHRLPLGGNVYVISVLENHSRAMLSSMLSRSQDLSAYLSVL
                     YAAVERYGSPEALVTDGGAVFRANQALSIYEVLNIRKEEIERGRPWQSYIETTFNVQR
                     RMADFHFAKAESWSELVAAHERWVGDYNEQSHWAHRERPDGRRSPSEVLGWVSGVRYR
                     EEDLRHAFFSTRFSRTLDSLGYARFRHWRIYGEEGLSRREAALWLGSESLTLEYAGEL
                     LSCYQVEYEPGSSRLVAISRPTLYETSHRLLAQLRLLELGDEEWLKALKLEDYAPRRP
                     RKPQALQQALFTYTEAI"
BASE COUNT          679 a         1316 c         1224 g          720 t
ORIGIN      
        1 tcatcagatg gcccttcagg tagtagcggc ccccgggggc agcgcggggt cgagggccgc
       61 ctggatggcg gcgtagggca tcggctggat ctcgtccacc agtggagcgc cgaacgtccg
      121 catcggttgc actgctcgct cgcccgcgtc gaagggtccg ttccagcagc cgatcacgcc
      181 ggcgaccggc acgccttcgg gtgtgtggag aaaccccgcc agggtgacga gttcatccgg
      241 gacggctgcg acgaagtccc ggtagaagcc gaaaacggcg gccgcggcgg agaacgggtg
      301 gaagacgggc cccgcgagca ctcccgcgac aggatgcagc tgatactcaa acgacgtgac
      361 gatgccgaag ttgccgctgc cgccccgcag gccccagaag aggtcgtcat tggcggtcgg
      421 gctggcggtc acgacccgtc cgtcggcgag gacgacgttg gccgaggtga ggttgtcgca
      481 ggcgagcccg taccggccgg ccagccagcc gatgccaccg ccgagggtga gcccggcgac
      541 gccggtgtgg ctcacgattc cgccggtggt ggccagaccg tgcgcttgcg tctcgtgatc
      601 gacgtcggcc cacagcgcac caccctgcac ccaggccctg cggtcggcgg gatcgacccg
      661 cacggcccgc attgccgaaa ggtcaatgac gatcccgtcg tcgcaaacgg cggtgcccgc
      721 cacgttgtgg cctccgcccc gtatggcgat ctccagatcg tggtcgcggg cgaaacgcac
      781 ggccgcgacc acgtcggcgc tcccgatgca tcgggcgatc aggcgcgggc gccggtcgat
      841 ggcacggttc cacactgcac gggcgatgtc gtagtcggcg tggtcggcac tgatcagccg
      901 gcctcggaac ccgtcgatct ccacgacggt tggctccttg ctcccttccg cggtattcgg
      961 tgtcggcatc gggtcctcct ctctcgacgg ccacatgtga ccaccgcgga ccccggccgt
     1021 caagtcaaga atctggactt cacaaccgaa ggcgggtgcg caatactagg gtcgtgagga
     1081 cgtacggcca atactgcccc atcgcccgtg gcgccgagat cttcgccgag cgctggacac
     1141 cgctgatcat ccgcaacctg taccttggct gcggaagctt cagcgagatc ctggagggcg
     1201 cgcctggact ctcccgtacc ctgctctcac agcggctcaa gcagctcgaa cgcgtgggca
     1261 tcgtcgagtc ggcacccaag cccgacgggc gcgggcacca ctacaagctc acgtccgcgg
     1321 gccacgagct cttcacggtc tgccagtcgc tcggcgagtg gggtgcgcgt tggctcgaga
     1381 tcgcccccga gaacctcgac ccctttgtgg ccctgtggtc gatgtgcaac gcgctccgcc
     1441 gagaccggct cccggatcga cgcgtcgtca tccgcttcga cttcaccggc cgcccgcgcc
     1501 gtgaacgtta ttggctactc atcgagctcg gagacaccga aatctgcaag aagtaccccg
     1561 gcctcgacga ggacctctac atcacggcgg aagccgaagc cttcgtcaaa tggcacgccg
     1621 gccagctcac ctgggcccaa gccacccgtg agggccgcat ccaactcgat ggcccctcgt
     1681 cgctcgtaag agccttccca acctggaacg cccgcagcat gttcgcccac atcaggccgg
     1741 tatcccgcac cctcacgagc cccgcaggct gacatcctcg acgcggcctc ccactgcggc
     1801 accgccgccg gaccctaagg aagggggcaa acctcaccat aggtggcgct acatatgcct
     1861 gtaccctcga tggcaggaat agagctcgtc tttagcgcca gcgtgcacca aagacaacag
     1921 aaaaaagtcg ttgcctccgt ccctaaatgg cctccgtgta ggtgaagagt gcctgctgca
     1981 gggcctgggg tttccgcggt ctcctaggag cgtaatcttc caacttcaga gccttcagcc
     2041 actcctcgtc gcccagctcc aggagacgaa gctgcgccaa taagcggtgc gacgtctcgt
     2101 acagggtagg ccgactgatc gccaccaacc ggctactgcc cggctcgtac tctacctggt
     2161 agcaggagag gagctctcca gcatactcca gcgtgaggct ttcgctcccg agccacaggg
     2221 cggcctccct cctggagagt ccttcttcgc catagatgcg ccagtgtctg aagcgggcgt
     2281 agcccaaaga gtcgagggtg cgtgagaagc gcgtggagaa gaaggcgtgc ctgaggtcct
     2341 cctcccggta gcgcactccg ctaacccacc cgagaacctc cgaaggcgag cgacggccat
     2401 cgggacgttc gcggtgggcc cagtggctct gctcgttgta gtctccaacc cacctctcat
     2461 gcgccgcgac gagctcggac cagctttccg ctttggcgaa gtgaaagtcg gccatcctgc
     2521 gctggacgtt gaaggtggtt tcgatgtagc tttgccaggg ccttccccgc tcgatctctt
     2581 ccttgcggat attcaggacc tcgtagatcg agagcgcctg gttggctctg aagaccgctc
     2641 cgccatcagt gacgagggct tccggggagc cgtatctctc taccgccgcg tacaggacgg
     2701 agaggtaggc cgagaggtcc tggctcctgc tcaacatgct ggagagcatc gcgcggctgt
     2761 ggttctccaa aaccgagatg acgtagacgt tccctcccag cggtagccgg tggtccacgt
     2821 agcggacatc ggcgctccag tactcgcgtc gtctcgaaga ggcaaaaggc atccgcctct
     2881 tctggtgcga gccccttttg ggtttgtcca gagcgtacag acggcggttt agcgccagga
     2941 ccctcccgca agttcggggg ctgaggtgga tgcccatccg tttcaaggcg ccatggatcc
     3001 gggactcccc gagctcgggg ttctgctgta ggcgcctgac ggccgcgatc gccttcaggt
     3061 ctacctttcg tacccctccg cccgcgccct cctttttggg cttgtcttcc agaccctcga
     3121 ggccctcctc gatgaaccta cgcaacgccc ggtagacggt agacctgtag gtcttcaggt
     3181 aggaagctat gctcttcact ttccagccct ccgcgtgaag cctaacgatg gccatcctgc
     3241 gctctttcgc ctcggggatc tcgtggtagg gcggatagcg cctgaccatc ctcaggggta
     3301 taggctcctc ctgaagcacc cgcgctacgg tgcgaccgtc gggatgcctg ccgaacctca
     3361 cgtagcagac gctggctatc tcgttggggc gcataggagg atgctcggcc ttcagatcga
     3421 cgatgaagcg gcgtatagca ggcggcaaac cgaagcgctt ggcgcgctcg gttgagaaca
     3481 ggctgtccat gccctcggcc tcgaagcgct tcactctccg gtacagtgtt cgttgggcgg
     3541 taccggtttg ctcggtgcgc tgtgtcaccg aagagccgaa caggaccagc ggtcggatct
     3601 cttcgtagga cacctgctcg ggccacccgc acagaggctt gagctgctcc cagtcgtcgg
     3661 tcggttcgac ccggtgccgt ctcttctttc ccatgcttct ttgcccccct cgttccttac
     3721 tgtgggggtg gaagcatgcc acgtccggaa atctgccgca gctaccttac gcttacagat
     3781 cgtggtgttt gggcgcctcg tttctgccaa gggagcaaca gatatgtgta gcgccaccta
     3841 tggcgaggtt tgcttctcag agactccggg tgaacgaggc acgaaaagag ccagggttgt
     3901 tagaagccct ggctcttgct taccggctgc caaccaaag
//
LOCUS       DATMVF010000250         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_627820, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000250 DATMVF010000000
VERSION     DATMVF010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627820"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..2317)
                     /locus_tag="VN178_12720"
     CDS             complement(284..2317)
                     /locus_tag="VN178_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81877.1"
                     /translation="MDPGGRDRGVWASERVYRALIRLYPEEVRRRYAEEMVRYFEDLC
                     WEERDSRGAKGMVLLWARTLPDLVFSVLKERGTRFHRNAYLPAEPGTAAKWGALSALL
                     GGSLGIAYSLAYTLAIFVPLDWLWRGWVTMPVLMFATLLSVLGTFGLYGTLVVHSGRP
                     DALSLSGATLGALSAVSILALYAYTTAEMLGWLWTPGEGTSWWEMQRTVLLQEIGMGA
                     CVLGLLLLGVSAFRARLFGRLRALPLAVAPLWPASIGLLILLNRSGMEYLASLSGTLP
                     FFGAALSGWVMLTYHPTERAVVAGGAPGSVSEVGNYGSTSGERATAGPRRSSRQWNVP
                     RARAEWLLLALSLLLMCVGAYAVRDTLLKTFGGMPPVLNEGPLSDESQRLGPQKTASG
                     ARVTLKQAYAEEKAVVIGVVMEDLEGDRSIAGHPAELEPDFRLTDESGTEFRLDSLGG
                     HVGATDAPKSFKAGFNAEEGLEPAQKHRFRLEVSLVEAVTPSRDFWGSNGEERPPPEP
                     VGKPFVFDFRVPVRGAPVVEVSQKDTASGVTLTLDRVNDSPGKPEAIVCYEAPDDEHS
                     WFISGGEGTYAAGDWWGTEIQGVPPAKCQTLQLKGPLEGRTLVEVTSLEGMPDCRAAK
                     AEALEACDKKIGEKTIRGPWRFEFEVPDPAGGHPAPRDAPPVPREADGGRGGA"
BASE COUNT          434 a          838 c          716 g          410 t
ORIGIN      
        1 catcgcggcc gcgatgatca ccggtagata gagcgtccta cttgccatgc ccctcctctt
       61 ctgttctcca tgcgcctata tttctgtaat agcaccatga catgcgttcc ctagagcacg
      121 acctccttcc ctaatgccag ctcgcctacg ccagagcgcc aaacgccctc atgggggagc
      181 gttgcgttcc cagaggccat gcaacagcct gccgaactcg aggtggccct cgggcgtaag
      241 cgcctgtggc acccgatcgg cctcctacac tgtaagcgag ctctcaagcg ccgcccctgc
      301 cgccgtcagc ttcccgcggc acaggaggag catcccgcgg cgctggatga cctccagcag
      361 gatcgggaac ctcaaactcg aacctccagg ggccacggat cgtcttttct ccgatcttct
      421 tgtcgcatgc ctctaaggcc tcggctttcg cagcacggca atccggcatg ccctcgaggc
      481 tcgtcacttc taccaacgtg cggccctcca gcggaccttt caactgaagc gtctggcact
      541 tggcaggagg gacgccctgt atctccgtac cccaccagtc acccgccgcg tacgtaccct
      601 ccccgccaga gataaaccag gagtgctcgt cgtcgggggc ctcgtaacag acgattgctt
      661 cgggcttccc cggcgagtcg tttacccgat ccagtgtgag cgtgacgcca ctggccgtgt
      721 ccttctgact gacctcgacg acgggagcac cccgcacggg gacccgaaag tcgaagacga
      781 agggtttccc gaccggttcg ggaggcggtc gctcctctcc gttcgagccc cagaaatctc
      841 gcgagggcgt caccgcctcg acgagggaga cctcgaggcg gaagcggtgc ttttgggcag
      901 gctcgagtcc ctcctctgcg ttgaaacctg ccttgaagga tttgggcgcg tccgtcgcgc
      961 ctacatgtcc tccgaggctg tccagcctga attcggtgcc gctctcgtcg gtgagtcgga
     1021 agtcgggctc gagctcagcg gggtgtcccg cgatgctgcg atctccttcg agatcctcca
     1081 tcacgacgcc aatgacgact gccttctctt cggcgtatgc ctgcttcagg gtgaccctcg
     1141 ccccactcgc tgtcttctgc gggccgagcc tctggctttc gtctgagaga ggtccttcgt
     1201 tgagcacggg cggcatgccc ccgaaggtct tgagaagcgt gtcgcgaacg gcgtaagcgc
     1261 ccacgcacat cagcaacagc gagagagcca ggagaagcca ctccgcacgc gctctaggca
     1321 cattccactg gcggctcgaa cggcggggtc cggccgtcgc tcgctcccct gacgtggatc
     1381 cgtagttccc tacctcagag acgctgcccg gcgcgccgcc ggccactacg gcgcgttcgg
     1441 tgggatggta cgtgagcatt acccagcctg agagcgccgc gccgaagaag ggcagggtgc
     1501 cgctgagcga tgcgaggtat tccatcccgg acctgttcaa cagtatgaga aggccgatgc
     1561 tcgccggcca cagcggggcg acggccagcg gcagggcgcg caggcgcccg aacaggcgtg
     1621 ccctgaaggc gctcaccccc aacagcagca gcccgagcac gcaagcgccc atgccgatct
     1681 cctgaaggag cacggttctt tgcatctccc accaggacgt gccctcacca ggcgtccaca
     1741 accatcccag catctcggcc gtcgtgtaag cgtagagcgc caagatggag accgcagaca
     1801 gagcgccgag cgtcgcgcca gagagggaga gcgcatccgg gcggcccgaa tgcacgacca
     1861 gcgtgccgta caggccgaac gtaccaagaa cggagagaag cgtcgcgaac atgaggacgg
     1921 gcatggtgac ccacccgcgc cacaaccagt ccaggggaac gaagatagcc aaggtgtatg
     1981 ccaggctgta ggctattccc agcgagccgc ccaggagtgc cgaaagggcg ccccacttcg
     2041 ccgcggtccc tggctccgcg ggaaggtagg cattcctgtg gaaccttgtg cccctttcct
     2101 tgaggacgct gaagaccaga tcgggcagcg tgcgagccca cagtaacacc atccccttag
     2161 cacccctgct gtccctctct tcccagcaaa ggtcttcgaa atagcggacc atctcctctg
     2221 cgtagcgccg ccgcacctct tcggggtaga ggcggatcag tgcccggtag acgcgctctg
     2281 aggcccaaac gccccgatct cgcccgccgg ggtccacctc aggcctcccc gggggaggtc
     2341 acgccgcgtc ccgagggcgg aaagagcccc ttctgctggg ccgtccttac caccctct
//
LOCUS       DATMVF010000251         8573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_676131, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000251 DATMVF010000000
VERSION     DATMVF010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676131"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..4036
                     /locus_tag="VN178_12725"
     CDS             173..4036
                     /locus_tag="VN178_12725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWS81878.1"
                     /translation="MDINKLEKISIGLAAPDEIREWSRGEVTKPETINYRTLRPEKDG
                     LFDERIFGPSKDWECYCGKYKRIRFKGIICERCGVEVTRARVRRDRMGHIELAAPVAH
                     VWFVKGVPSRMGYLLDISPKELDRVLYFASSIVTWVDREERANDIDLLRGRVEDEIQQ
                     LELDRDEEIDQIQALRTKRESVIKGESSPDELTEEYADIPEDEREAAITKVHKEAEES
                     VADIGRSVTEQIGLVRRAWEEFQTLEPRQIVDDEELFREMKDRFGDEYGYGVYFRGGM
                     GAEAVRDLISQVDLENEAQDLRDVVASSRGQKRQKAIKRLKVVDAFRVSGNGPEWMIM
                     EAIPVLPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLRRLLDLGAPDVIVNNEKR
                     MLQEAVDALFDNGRRGRAVTGAGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVI
                     VVGPGLKMHQCGLPRLMAVELFKPFVMKVLVDRALAPNIRSAKQMVERLRPEVWDVLE
                     DVIKEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIQVHPLVCSAFNADFDGDQMAVHVP
                     LSPEAQAEARVLMLSTQNILLPANGFPIAVPSQDMVLGLYYITYGPENFEEAEVKALI
                     SSYDEAEAAYKEGTLKIHDKVIARRGGERMETTLGRVLFNENVEQTLRDYLGDAYDPD
                     AYPYINHTLKKGEIKDLVAGYVARYPTEVVSQLLDTLKRVGFHTATKAGISVGKNDIV
                     VPEQKEEILERYQSMVDEIEDQWDQGFISDDERLERVIGLWTQAKNEVEQAMKDNLWR
                     LNPIYMMADSGARGNYNNFTQLAGMRGLMTNTKGEIIEEPVKSNFMEGLSVLEYFTST
                     HGARKGQADTALRTADSGYLTRRLVDVSQDVVVNDEDCGSDGYVDLPLYLEGGRGDAN
                     DSVAARFLARDLVNPETGEVVAEAATDITPGLLATWREELPRETLVPVRTPSKCESPH
                     GVCQTCYGRALSTYRPVDVGEAVGIIAAQSIGEPGTQLTMRTFHTGGIAGEDITAGLP
                     RVVELFEARHPKAEATLAEIDGIVSLEEADSDRQIRVVITAPDGDEERSYTVERQLLR
                     PEFVEGAEVRANTPITEGSIYPHAILENDLRYGRGSTMTERYLVDEVQKVYRAQGVDI
                     HDKHIEVIVRQMLRKVIVDDPGDTNLLPEQVADRFTVMAENARVEEEEGTQATYHTVL
                     MGITKASLATDSFLSAASFQETARVLTDAAIEGKVDSLSGLKENVIIGKLIPAATGLP
                     RYRRLTVAVEGYRAQDIDELDIAAS"
     gene            4356..4724
                     /gene="rpsL"
                     /locus_tag="VN178_12730"
     CDS             4356..4724
                     /gene="rpsL"
                     /locus_tag="VN178_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWS81879.1"
                     /translation="MPTTNQLVRNEREKQTKKAATPALQGSPQKRGVCTRVSTTTPRK
                     PNSALRKVARVRLVNGQEVTAYVPGEGHNLQEHSVVLVRGGQVKDLPGVRYKVVRGAL
                     DTLGVNNRKQGRSKYGTKKS"
     gene            4736..5206
                     /gene="rpsG"
                     /locus_tag="VN178_12735"
     CDS             4736..5206
                     /gene="rpsG"
                     /locus_tag="VN178_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWS81880.1"
                     /translation="MPRRAAPPKKKIAADPVYGSVLVQQLVNKIMLDGKKSTSEKIVY
                     DALSYIEERTGTSPVQVLNNALDNIRPRLEVKSRRVGGATYQVPVEVDARRANTLALR
                     WMVGFARARREKTMGRRLAGEILDAKDNIGSSVKRKEDTHRMAEANRAFAHYRW"
     gene            5219..7363
                     /gene="fusA"
                     /locus_tag="VN178_12740"
     CDS             5219..7363
                     /gene="fusA"
                     /locus_tag="VN178_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWS81881.1"
                     /translation="MAVQVSRKIPLKRVRNIGIMAHIDAGKTTTTERILLYTGLTHKL
                     GEVHDGNAVMDWMAQERERGITITSAATTVFWGGIEGSSKNGKGTREGVHSRIADQHR
                     INIIDTPGHVDFTVEVERSLRVLDGAIALFDSVAGVEPQSETVWRQADKYGVPRIAFV
                     NKMDRIGADFYKAVGTMVDRLGANAVPVQLPIGAEGEFQGIIDLVEMRAIVYTDDLGA
                     TWEVTEVPEEMRALADEYRERLLEAVADQDEELMVMYLEGEEIEPDQIRAAIRQATLD
                     IRMTPVFVGSAFKNKGVQPLLDGVVDYLPSPLDVPPVEGKTLDGEEITREADENGPLA
                     ALAFKVQADPHVGKLTYVRVYSGTLKAGSYVMNTTKGRRERVGRLLQLHANSREQRDE
                     VYAGELVAAIGLSDTGTGDTLVSADDEQIVLEEMIFPEPVIRQAIEPKTKADQEKLSV
                     ALQRLAEEDPTFTVRSDEETGQTIVAGMGELHLEIIVDRLLREFRVDANIGRPQVAYR
                     ETIRKRVEGIEGRFVRQTGGRGQYGHAVINAEHNDEAGYEFDNKIVGGVIPREYIPSV
                     DRGIKEALDSGVVAGYPVVDVRVELVDGSYHEVDSSEMAFQIAGSMAIKEALKRANPV
                     LLEPVMDVEVVVPEEFMGDVMGDLSARRGHIQGMDSRGGGQVIRAMVPLSEMFGYATT
                     VRSKTQGRATFTMQFDHYAEVPKSISAEIAER"
     gene            7372..>8573
                     /gene="tuf"
                     /locus_tag="VN178_12745"
     CDS             7372..>8573
                     /gene="tuf"
                     /locus_tag="VN178_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWS81882.1"
                     /translation="MSRGRFERTKPHLNVGTIGHVDHGKTTLTAAITRVLAKEVPDDP
                     ANREVAFDQIDNAPEERQRGITIATSHQEYATANRHYAHVDCPGHADYVKNMITGAAQ
                     MDGAILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKADMVDDPELLELVEMEVRE
                     LLSEYEFPGDDIPVVIGSALRALEGDDSEYGEPSVLALMEAVDEYVPEPERDIDRDFL
                     LAVEDVFTIQGRGTVATGRVESGKIGLNEEIEIVGIRPTRKTVVTGVEMFNKSMDSAQ
                     AGDNIGALLRGVKRDDIERGQVLAKPNSITPHTLFKAEVYVLSKEEGGRHTPFFSNYR
                     PQFYFRTTDVTGEIRLEEGVEMVMPGDNTVMNVELISPIAMDEGLNFAIREGGRTVGA
                     GVVTEIVE"
BASE COUNT         1845 a         2579 c         2809 g         1340 t
ORIGIN      
        1 tgtctacaac gcggaggcag tggcagagga acatgagcgc agggattggg acgaggcggc
       61 aagggcgttg gggataaacc tcagccgtga cgaacctacg ggcgacctgg aggacccacc
      121 tggagccttc ggcagaggag ggatgtagag cgtgcaggga ctcgagcgcg acatagacat
      181 aaacaaactg gagaagatct ccatcggcct ggccgccccc gacgagatca gggaatggtc
      241 gaggggtgag gtaaccaagc ccgagacgat caactatcgc accctacgcc ctgagaagga
      301 cggtctgttc gacgagcgca tcttcggccc ttccaaggac tgggagtgct actgcggcaa
      361 gtacaagagg atccggttca agggtatcat ctgcgagcgg tgcggagtcg aggtaacccg
      421 tgcccgggtg cgccgcgaca ggatgggcca catcgagctc gccgcccccg tggcgcacgt
      481 gtggttcgtc aagggcgtcc ccagccggat gggctacctc ctcgacatca gccccaagga
      541 gctcgaccgg gtactctact tcgccagctc catcgtcacg tgggtcgacc gcgaggagcg
      601 ggccaacgac atagacctgc tacgcggcag ggtggaggat gagatccagc agctcgagct
      661 ggacagggac gaggagatcg accagatcca ggccctcaga accaagcgcg agagcgtgat
      721 caagggcgag agctctccgg acgagctgac cgaggagtac gcggacatac ccgaggacga
      781 gcgcgaggcc gccataacga aagtccacaa ggaggccgaa gagtccgtcg cggacattgg
      841 tcgctcggtt accgagcaga tagggctcgt caggcgggcc tgggaggagt tccagacgct
      901 ggagccgcgc cagatcgtgg acgatgagga gctcttccgc gagatgaagg accgtttcgg
      961 cgacgagtac ggctacgggg tgtacttccg cggcgggatg ggcgcagagg ccgtaaggga
     1021 cctcatatcg caggtcgacc tcgagaacga ggcccaggat ctcagggacg tcgtggcctc
     1081 ctcgagagga cagaagcgcc agaaggcgat caaacgcctc aaggtcgtcg acgccttccg
     1141 cgtcagcggc aacggcccgg agtggatgat catggaggcc atcccggtgc tcccgccaga
     1201 cctccgcccc atggtccagc tcgacggcgg ccgtttcgcc acctccgacc ttaacgacct
     1261 ctaccgcagg gttataaaca ggaacaacag gcttcgtcgc ctgctcgacc tcggcgcacc
     1321 ggacgtgatc gtgaacaacg agaagcggat gctgcaggag gccgtggacg ccctcttcga
     1381 caacggtcgt aggggtcgcg ccgtcaccgg cgccggaaac agggcactca agagcctctc
     1441 ggatatgctc aagggcaagc agggccggtt ccgtcagaac ctgctcggca agcgcgtgga
     1501 ttactccggc cggtcggtca tcgtggtcgg ccctggcctg aagatgcacc agtgtggcct
     1561 cccacgcctg atggccgtcg agcttttcaa gccattcgtg atgaaggtcc tggtcgacag
     1621 ggccctggcg ccgaacatcc gtagcgccaa gcagatggtc gagcggctac gtccggaggt
     1681 ttgggacgtc ctcgaagacg ttatcaaaga gcacccggtg ctattgaacc gcgcgccgac
     1741 gcttcaccgt ctcggcatcc aggcgttcga gccagtgctc gtggagggca aggccatcca
     1801 ggtacatccg ctggtctgtt ccgccttcaa cgcggacttc gacggcgacc agatggcggt
     1861 gcacgtgccc ctgagccccg aggcgcaggc tgaggcccgc gtcctcatgc tctccacgca
     1921 gaacatacta ctgcccgcca acgggttccc gatcgccgtt ccctcgcagg atatggtgct
     1981 cgggctctac tacatcacct acggtcccga gaacttcgaa gaggccgaag tcaaggctct
     2041 catctcctct tacgatgagg ccgaggccgc ctacaaagag ggtacgctca agatccacga
     2101 caaggtcata gcccgcaggg gcggtgagcg gatggagacc acgctcgggc gggtcctttt
     2161 caacgagaac gtcgaacaga ccttaagaga ctacctcggc gacgcctacg accccgatgc
     2221 ctacccctac atcaaccaca ccttgaagaa gggtgagatc aaggacctcg tcgccgggta
     2281 cgtggcccgc tacccgacgg aggtcgtcag ccagctcctc gacaccctga agagggtcgg
     2341 tttccacacg gccaccaagg ccggcatctc ggtcggcaag aacgacatcg tcgtgcccga
     2401 gcagaaagag gaaatcctcg agcgctacca gagcatggtc gacgagatcg aggaccagtg
     2461 ggaccagggc ttcatctccg acgacgagag gctggagcgt gtgatcggac tgtggaccca
     2521 ggccaagaac gaggtcgagc aggccatgaa ggacaacctc tggcgcctga acccgatcta
     2581 catgatggcc gactcgggcg cccgcggcaa ctacaacaac ttcacccagc tcgcaggcat
     2641 gcgcggcctt atgacgaaca cgaagggtga gatcatcgag gagcccgtga agagcaactt
     2701 catggagggc ctctcggtgc tggagtactt cacctcgacc cacggagcga ggaagggcca
     2761 ggccgacacg gccctgcgta ccgctgactc cggctacctg acccgccgcc tcgtcgacgt
     2821 ctcccaggat gtcgtcgtga acgatgagga ctgcggctcg gatggctacg tcgaccttcc
     2881 gctctacctg gaaggcggca ggggcgatgc gaacgactcg gtcgccgccc gtttcctggc
     2941 ccgggatctg gtgaacccgg agaccggcga ggtcgtcgca gaggcagcta cggacatcac
     3001 cccgggcctc cttgccacct ggcgcgagga gctgccacgc gagacgctcg tccccgtgcg
     3061 gacgccctcc aagtgcgaga gcccgcacgg cgtatgccag acctgttacg gcagggcgct
     3121 ctccacctac agacccgtcg acgtcggtga ggccgtcggc atcatcgcgg cccagtctat
     3181 cggggagcct ggcacccagc tcacgatgcg tacgttccac accggcggca tcgccggcga
     3241 ggacatcacg gccggcctgc caagggtcgt cgagctcttc gaggctcggc atcccaaggc
     3301 cgaggcgacc ctcgccgaga tagacggtat cgtctccctg gaggaggccg actccgacag
     3361 gcagataagg gtcgttatca ccgcgccaga tggcgacgag gagaggtcgt atacggtcga
     3421 gcgccagctc ctcaggcccg agttcgtcga gggcgccgag gtcagggcga acaccccgat
     3481 cacggagggg tctatctacc cgcacgccat cctggagaac gacctccgtt acggccgcgg
     3541 cagcaccatg accgagcgct acctggtcga tgaggtgcag aaggtctacc gagcccaggg
     3601 cgtggacatc cacgacaaac acatcgaggt catcgtacgc cagatgctgc gcaaggtgat
     3661 cgtggacgac ccgggcgaca ctaacctgct ccccgagcag gtcgccgacc gctttaccgt
     3721 catggccgag aacgcccgcg tcgaggagga ggaaggcact caggccacct accacacggt
     3781 cctgatgggt ataaccaaag cctcgctcgc caccgacagc ttcctctccg cagcctcttt
     3841 ccaggagacg gcacgcgtcc ttaccgacgc tgccatcgag ggcaaggtag acagcctctc
     3901 aggcctgaag gagaacgtca ttatcgggaa gctcataccc gcggccacag gactgccccg
     3961 ttacaggcgg ctcaccgtgg ccgtcgaggg ctacagagcg caagacatcg atgagctcga
     4021 catagcagca tcctagctgt cagcttgtca gcgatcagcc ctcagcttct cttgctgggg
     4081 gctgaccgct tttcgacaag gcaacgcggt cgtgctagtt ccacggcgcg gcccacaagg
     4141 cagcagcatc gaacccgacc ggagaagcta tcggcggggc aaagctgaca gctgaaggct
     4201 aatggctgac cgctctaaga gcttgacata gcgctcttct gagcctaaaa tattgcgtcg
     4261 cggttacgag tcgttaccaa tgggtaacaa aggctctgtg ccgtggcacg tgatcctgta
     4321 agcccctttt catggataga gagagaggcg cggccatgcc gacgaccaac cagcttgtgc
     4381 gcaacgagcg ggagaaacag acgaagaagg cggcgacgcc ggcgttgcag gggtctcccc
     4441 agaaacgcgg cgtgtgcacg cgtgtctcga ccaccacccc gaggaagccg aactccgcgc
     4501 tgcgcaaggt ggccagggtc cgcctggtga acggccagga ggtcacggcc tacgttcccg
     4561 gtgaggggca caacctgcag gagcactctg tcgtgctcgt gcgtggggga caggtcaagg
     4621 acctgccagg cgtgcgttac aaggtcgtgc gcggggcgct cgatacgctc ggggtcaaca
     4681 accgcaagca gggccgttcc aagtacggca ccaagaagtc gtaggaggat aggagatgcc
     4741 gaggcgagca gcgccaccaa agaagaagat agccgccgat cctgtctacg ggagcgtgct
     4801 ggtgcagcag ttggtcaaca agatcatgct cgacggcaag aagagcacct cggagaagat
     4861 cgtctacgac gccctctcat acatcgagga gcgcacgggc acgagcccgg tccaggtgct
     4921 gaacaacgcc ctggacaaca tcaggccgcg cctggaggtc aagagccggc gcgtcggcgg
     4981 tgcgacctac caggtgcccg tcgaggtcga tgcccgaagg gcaaacacgc tggcgctgcg
     5041 ctggatggtg ggcttcgccc gtgccaggcg cgagaagacc atgggccgca ggcttgcagg
     5101 cgagatcctg gacgccaagg ataacatcgg ctcgagcgta aagcgtaagg aagacacgca
     5161 tcggatggca gaggcaaaca gggccttcgc ccattacagg tggtgacgag gaactaatat
     5221 ggctgtacaa gtatccagaa agatcccgct aaaacgggtc cgtaatatcg gcatcatggc
     5281 ccacatagat gccggaaaga ccacgacgac agaacgcatc ctgctctata cgggccttac
     5341 ccacaagctg ggcgaggtcc acgacggtaa cgccgtcatg gactggatgg cccaggagcg
     5401 cgaacgcggc atcactataa cctcggccgc aacgaccgtg ttctggggcg gcatcgaagg
     5461 ctccagcaag aacggcaagg gtacccgcga aggcgtccat agccgcatcg ccgatcaaca
     5521 tcgtatcaat attatagaca cgcccgggca cgtggacttc actgtagaag tggagcgcag
     5581 ccttcgggtg ctggacggtg cgatcgcgct gttcgactcc gtggccgggg tcgagccgca
     5641 gtccgagacg gtctggcgcc aggcggacaa gtacggggta ccgcgcatag ctttcgtcaa
     5701 caagatggac cgtattggcg ccgacttcta caaggccgtt ggcacgatgg tcgacaggtt
     5761 gggggcgaac gccgtccccg tgcagttgcc tataggcgcg gagggcgagt tccaggggat
     5821 catagatctc gtagagatga gggccatcgt ctacaccgac gacctcgggg ccacgtggga
     5881 ggtcaccgag gtaccggagg agatgcgtgc cctcgctgat gagtacagag agaggctcct
     5941 ggaggcggtc gccgaccagg acgaagagct catggtcatg tatctggagg gcgaagagat
     6001 cgaacccgac cagatccggg ccgccatccg tcaggccacg ttggatataa ggatgacgcc
     6061 cgtcttcgtc ggttcggcgt tcaagaacaa gggcgtgcag ccactcctcg acggggtagt
     6121 cgactacctc ccgagcccgc tcgacgtgcc gcccgtggag ggcaagaccc tcgacgggga
     6181 ggagataacc cgcgaggcgg acgagaacgg cccgctcgcg gcgctggcct tcaaggtcca
     6241 ggcggatccg cacgtcggga agctgaccta tgtgcgggtg tactcgggca cgctcaaggc
     6301 cggttcctac gtgatgaaca ccaccaaggg caggcgcgag cgcgtaggac gtctgttgca
     6361 gctgcacgcg aacagtcgcg agcagcgaga tgaggtctat gccggggagc tcgtcgcggc
     6421 catcggcctc tccgatacgg ggacgggcga cacgctcgtc tcggcggacg acgagcagat
     6481 cgtgctggag gagatgatct tccccgagcc cgtgatccgg caggctatcg agcccaagac
     6541 gaaggccgac caggagaagc tctccgttgc gctccagcgg ctcgccgaag aggaccccac
     6601 gttcaccgtg cgcagcgacg aggagacggg acagaccatc gtcgccggca tgggcgagct
     6661 tcacctagag atcatcgtcg acaggctgct gcgcgagttc agggtcgatg cgaacatcgg
     6721 ccgcccgcag gtcgcttacc gcgagaccat ccgcaagcgc gtggagggca tcgagggccg
     6781 gttcgtgcgg cagaccggtg gccggggcca gtacggccac gcggtcatca acgccgagca
     6841 caacgacgag gcgggttacg agttcgacaa caagatcgtc ggcggcgtca tcccgcggga
     6901 gtacatcccc agcgtggaca ggggcatcaa ggaggcgctg gattccggcg tcgtcgcggg
     6961 ctaccccgtg gtcgatgtca gggtcgagct ggtcgacggt tcttaccacg aggtcgactc
     7021 ctccgagatg gcgttccaga tcgccggttc catggccatc aaagaggccc tcaagagggc
     7081 caacccggtc ctactcgagc ccgtgatgga cgtcgaggtc gtagtgcccg aagagtttat
     7141 gggcgacgtg atgggcgacc tttcggcgag gcgcggccac atacagggta tggactcccg
     7201 cggtggcggg caggtcatcc gcgccatggt gccgctctcc gagatgttcg gttacgcgac
     7261 gaccgtgcgc tccaagaccc agggccgcgc tacgttcacc atgcagttcg atcactatgc
     7321 cgaggtgccg aagagcatat cggccgagat agcagaaagg taggagaggc aatgtcaaga
     7381 ggcaggttcg agcgcaccaa gccgcaccta aacgtcggca ccatcggtca cgtcgaccac
     7441 ggcaagacca ccctcaccgc ggccattacc agggtcctgg caaaggaggt ccccgacgac
     7501 cccgcaaacc gtgaggtggc attcgatcag atagacaacg cccccgagga gcgtcagagg
     7561 ggcattacta tcgctacttc tcaccaggag tacgccacag ccaaccgcca ctacgcccat
     7621 gtggactgcc cggggcacgc cgactatgta aagaacatga tcacgggggc ggcccagatg
     7681 gatggggcta tcctggtggt cagtgcggca gacggtccca tgccccagac aagagagcac
     7741 atcctcctgg ccaggcaggt aggtgttccg tacatagtgg tctacctcaa caaggccgac
     7801 atggtcgacg accctgagct tttggagctt gtggagatgg aggtaaggga actcctctct
     7861 gagtacgagt tcccaggaga tgacatcccc gtggtcatag gcagtgcctt gagggcattg
     7921 gagggtgacg attctgagta tggggagccc tcggtgttgg cgctcatgga ggcggtagac
     7981 gagtacgtcc ccgagcccga gcgcgacata gaccgcgact tccttttggc tgtagaggat
     8041 gtgttcacca tccagggcag gggcactgta gccacaggaa gggtagagtc aggcaagata
     8101 ggactaaacg aggagataga gatagtaggc atccgcccca ccagaaagac ggttgttacg
     8161 ggggttgaga tgttcaacaa gtcgatggac tcggcacagg ccggggacaa cataggggct
     8221 ctattgaggg gggtaaagcg cgacgacata gagcgcggac aggtattggc caagcccaac
     8281 tccataacac cgcacaccct cttcaaggca gaggtatatg tgctttctaa ggaggaggga
     8341 ggcaggcaca caccgttctt ttccaactac cgtccacagt tctacttcag gacgacggat
     8401 gtgacggggg agatccgact ggaggagggg gtagagatgg tgatgcctgg agacaacacg
     8461 gtaatgaacg tggagttgat cagtcccatc gcgatggacg aggggttgaa ctttgccatc
     8521 agggagggtg gcaggacggt tggggcaggc gtcgtaacgg agatcgtgga gta
//
LOCUS       DATMVF010000252         5675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_627847, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000252 DATMVF010000000
VERSION     DATMVF010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627847"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VN178_12750"
     CDS             complement(<1..253)
                     /locus_tag="VN178_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWS81883.1"
                     /translation="MRTQVGIVGGGPAGLVLSHLLHLQGIESVVLELRSRHVVETTVR
                     AGVLEHGTAELLRETGVGERMDREGAVHNGVNLQFGGRRH"
     gene            complement(311..1795)
                     /locus_tag="VN178_12755"
     CDS             complement(311..1795)
                     /locus_tag="VN178_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81884.1"
                     /translation="MEASTEVKTAPYPGFRTLWIWLGLGWVASGVDRTITGPVMTYMI
                     DNKVPLLQGVANPFAVSGLVGSLLFAGYMLMQFPGGYVGDKLGHRTVIVISIVWAGVA
                     TILSGLMTALIGLVALRVITGLGAGLFYSNDRSVITEQTPFEKRSLGMGFVITGLAIG
                     ITLAFLLAPPLIGFGTSVFGAEGAWRMPFIALGVAAVVIGIGMHVFFRGQGGEHEFRP
                     AYGAALARLGTYAAIFLVAVVGIYFVATRAGLPEWGVALLELGVALLLVGFIFARLGG
                     EISPVLYNRDLLLIYIAAIAILWNLWFFGFWSVSIIGDAAQSSFLQAALTAAFNAGAG
                     ILGFPAGGWLADYAKRKGWGRKPMLVSFTLIQGLLTLGFGFYIMNGGQSVLVLGALLF
                     TASLFFNALQPMSQALTADLVPSAAYLGAAFGLWNLIGEMGAVLSPAISGTLRDATGN
                     WTAAVMLDAGIILVSFVLLLFVREPREEVEAPARAQTPLEGEPG"
     gene            complement(1916..3565)
                     /locus_tag="VN178_12760"
     CDS             complement(1916..3565)
                     /locus_tag="VN178_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWS81885.1"
                     /translation="MKVYEAVAETLSRLGVETTFGLVGSGNFGLIDHMTRQCGIAYHA
                     SRHETAAVAMADGYARVSGKLGVCTVHQGPGVTNTLTAMTEAVKAGTPLLLLAGDIAT
                     TALYQNLDVDQDSVAGSVGAGTEEIRSANTAVEDIARAARRAKIERRPIEISLPIDIQ
                     EQECEAKEPPAFVEQEIAAPRPSEEAVSRVADLLESSSRPAIIAGRGAVLAGARPQLE
                     ALGDRIGALFATSMMAKGFFAGSRFDLGVSGGFASPLAARYLGESDLVLSFGASLNTW
                     TTKHGTLFSPSARIVHCDLEPSAIGRILPVSLGVVGDAAEIAEALLEELTRRNARSEG
                     FRTDSVRRNIQGFRWEDEFEDESTEDTVDPRAALMALEEMLPEERTVAVDCGHFAGFA
                     AMHLTVPDAAGFVFAEAFQAVGLGMGTGMGAAVARPDRLAVVVVGDGGLLMSLGEIDA
                     AVKMGVPMLVVVMNDAAYGAEVHHFRSLGLPTDLALLGGIDFAAIAGAMGASGLEVRG
                     DGDLKRLEEWLEEPDGVMVVDCKVNPRVEGEYLREAFVAEA"
     gene            complement(3746..4159)
                     /gene="pcaC"
                     /locus_tag="VN178_12765"
     CDS             complement(3746..4159)
                     /gene="pcaC"
                     /locus_tag="VN178_12765"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HWS81886.1"
                     /translation="MNPGESAHERGMKVRREVLGDEHVDAAIERQTDFTADFQDFITR
                     YAWGEIWIRPGLDRRIRSCITLTALVALGKLDELEMHVRAALRNGLTQDEIKEVLLQC
                     AVYCGVPAANSAFAVAQGVLADGGRHPEAKGDAPD"
     gene            complement(4156..4947)
                     /gene="pcaD"
                     /locus_tag="VN178_12770"
     CDS             complement(4156..4947)
                     /gene="pcaD"
                     /locus_tag="VN178_12770"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HWS81887.1"
                     /translation="MKAESAVEVHHLLEGPEDAPVLVLSNSLGTTLAMWDEQGPELSK
                     RFRLVRYDHRGHGASPVPPGPYRIEDLARDVLVFLDRHEVERFSFCGLSIGGMVGMWL
                     AGEAPERVERLVLCCTAARFPPDTFDVRARTVRADGIDAIADTVVDRWFTPAFREGQP
                     EIAGWARRMLVETPAEGYAGCCEAVRDTDLRDTLGTISSPTLVIAGADDLAAPPEGAK
                     SIRDSIPEARLEVIPDAAHLASIEQPEAVTQAVLDHLIPVVREWR"
     gene            complement(4944..>5675)
                     /locus_tag="VN178_12775"
     CDS             complement(4944..>5675)
                     /locus_tag="VN178_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWS81888.1"
                     /translation="VLAEFARELDLAEPVVPWHTDRSRIAQIGGTLSLLAGVLGKISL
                     DIILMAQTEVGEVSEPAGAGRGGSSTLPHKRNPILSVTAAACSRRVQDLARTLQSAMA
                     GEHERAAGAWHAEWEALSDALALTGGTAAAVREATEGLEVYPEKMKENLDASGGLLLT
                     ERVTTLVAEHLGRLKAHDLVEATSKRTLESGKSLRDELLAEPALVEVRSEENIDDALD
                     PAGYLGSTGAFVDRALGLYRKEVQA"
BASE COUNT         1002 a         1961 c         1763 g          949 t
ORIGIN      
        1 ggtggcggcg accaccgaac tgcaggttta cgccattgtg gacggcccct tcacgatcca
       61 tcctctctcc gacgcccgtc tccctgagca attccgccgt tccatgctcg agcacgccag
      121 cccggaccgt cgtctctact acgtggcggc tgcggagctc gagcacgacc gactcgatgc
      181 cctgcaggtg cagcaggtgc gagagcacca gcccagccgg cccgcctccg acgatgccca
      241 cctgtgtacg cattatgcgc tgctcctccg taactcgtcc ggctattatc ctcctgtcaa
      301 ggagctccgc tcaaccaggc tccccttcca agggtgtctg tgcccttgca ggcgcttcta
      361 cttcctccct cggttctctg acgaacaaga gcagcacgaa gctgaccagg atgatacccg
      421 cgtcgagcat caccgcggcc gtccaattgc ccgtcgcgtc ccgcagcgtg ccgctgatgg
      481 cgggcgagag gaccgctccc atctcaccga tcaggttcca aaggccgaac gccgcgccga
      541 ggtacgcagc acttggtacc aggtccgcgg tgagagcctg ggacatgggc tggagggcgt
      601 taaagaagag gcttgcggtg aataacaggg cgcccaacac gagcacggac tgtccgccgt
      661 tcatgatgta gaacccgaag ccgagggtaa ggaggccctg gatcagggtg aacgacacga
      721 gcatcggctt gcgtccccag cccttccgct tggcgtagtc ggccagccac ccgccggcgg
      781 ggaagccgag gatgccggct cctgcattga acgccgccgt cagcgctgcc tgtaggaacg
      841 aggactgcgc cgcgtcgccg atgatagaga ccgaccaaaa gccgaagaac cagaggttcc
      901 agaggatggc gatggccgcg atgtagatca gcaagaggtc cctgttgtag aggaccgggg
      961 aaatctcccc gcccaatctg gcgaagatga agcccaccag caggagcgcg acgccgagtt
     1021 cgagcaacgc gaccccccat tcgggcagcc ccgccctggt ggcaacgaag tagatgccca
     1081 ccacggcaac caggaagatg gcggcgtagg tgcccaacct cgccagagcc gccccgtagg
     1141 ccggcctgaa ctcgtgttcc cctccttgcc ccctgaagaa cacgtgcatg ccgatgccga
     1201 taaccacggc cgccactcca agcgcgatga acggcatccg ccacgcgccc tcggcaccga
     1261 agaccgaggt tccgaacccg atcagcggcg gggcgagcaa gaacgccagc gtgatcccga
     1321 tcgccaaccc cgtgatgacg aatcccatgc cgaggctgcg cttctcgaag ggggtctgct
     1381 cggtgatgac gctgcggtcg ttggagtaga aaagtccggc cccgagaccg gtgatcacgc
     1441 gcagggcaac tagcccgatt agggccgtca tgagcccact caggatggtg gccacgccgg
     1501 cccagacgat gcttatgacg atcacggtcc ggtgcccgag cttgtccccc acgtacccgc
     1561 ccgggaactg catcagcata taacccgcga acaacaggct gccaaccaac ccgctgacgg
     1621 cgaaggggtt cgctacgccc tgcaaaaggg gaaccttgtt gtcgatcatg taggtcatta
     1681 ccggtccggt gatggtgcga tcgacgccgc tcgccaccca tcccagtccg agccagatcc
     1741 ataacgtccg gaaccccggg tacggtgcag tcttgacttc ggtgcttgct tccacctgca
     1801 tctcctttcc cgctagcaac tctgctaggg ctttcgcgaa ttcccatccg agcgacacgc
     1861 gctccggttt cccccgcgcg gcgggcgcta cgtccggcac caccgaccgc gtcgtttacg
     1921 cctcggcaac gaacgcttcc ctcaaatatt ctccttccac ccgagggttg accttacagt
     1981 cgacgaccat gaccccgtcg ggctcttcga gccactcctc caaccgtttg aggtcgccgt
     2041 cgccacgcac ctcgagcccg ctcgcgccca tggcgcccgc tatcgcggcg aagtcgatgc
     2101 ctccgaggag cgcgaggtcg gtgggcaggc ccagggagcg gaagtgatgg acctcggcgc
     2161 cgtaggcggc gtcgttcatg accacgacga gcatggggac gcccatcttc accgccgcgt
     2221 cgatctcgcc gaggctcatc agcaacccgc catcccccac gacaaccacc gccaaccggt
     2281 ccggacgcgc cacggcggcg cccattcccg ttcccatgcc gagccccacg gcttgaaaag
     2341 cctcggcgaa gacgaatccc gcggcatccg gcacagtgag atgcatcgcg gcgaacccgg
     2401 cgaagtgccc acagtcgact gcgaccgtgc gttcctcagg cagcatctcc tcgagggcca
     2461 tgagcgcggc acgcgggtcc accgtatctt cggtgctctc gtcttcgaac tcgtcctccc
     2521 agcggaagcc ttggatgttt cgccttacgg aatccgtcct gaagccttcc gacctcgcgt
     2581 ttcggcgggt gagctcttcg agcagcgcct cagcgatctc ggccgcgtcg ccgacaacgc
     2641 ccagactcac gggcagtatg cgacctatcg ccgacggttc gaggtcgcag tggacgatcc
     2701 gggccgaagg ggaaaacagc gtgccgtgct tcgtggtcca agtgttcagg ctggcaccga
     2761 aggagagcac gaggtccgac tcgcccaagt agcgcgcagc gagcggggag gcgaaccccc
     2821 ctgagacgcc gaggtcgaaa cggctgcccg cgaagaagcc cttggccatc atcgacgtgg
     2881 cgaagagcgc cccgatccgg tctcccagcg cctccagctg cggacgagcg cccgccagca
     2941 ccgcgccccg cccggcgatg atcgcgggtc ggctcgagga ctccagcaga tcggcgaccc
     3001 gcgagacggc ctcctcggac ggtcgagggg cggcgatctc ctgctccacg aacgcgggcg
     3061 gttccttcgc ctcgcactct tgctcctgta tgtctatggg cagcgagatc tctatcggtc
     3121 ggcgctcgat cttcgcccgt cgcgcggcgc gggcgatgtc ctcgacagcg gtgttcgcgc
     3181 tgcgtatctc ctcggtgccg gcccccaccg aaccggcaac ggagtcctga tctacgtcca
     3241 ggttctggta gagcgcggtg gtcgcgatgt cgcccgccag caggagcaaa ggggtcccgg
     3301 ccttcacggc ctccgtcatg gcggtgaggg tgttggtgac acccggcccc tggtggacgg
     3361 tgcagacgcc cagcttgccg ctgacgcggg cgtagccgtc cgccatcgca acggcggccg
     3421 tctcgtgccg agaggcgtgg tatgcgatgc cgcattgcct cgtcatgtgg tcgataagac
     3481 cgaagtttcc gctgccgacc agcccgaagg tcgtctcaac cccgagccga ctaagggtct
     3541 ctgcgaccgc ttcgtacacc ttcatcctcg acccccctca tacgttctcc tctggagccc
     3601 cgggaggcac gggtagggca ccagaaggcg atcaagaccg ggcggaccga aatccgggtc
     3661 aaccggatag tgggcgtttg ttggtcttgc ggtctgaacc ataatcactt cccatccata
     3721 agcaacctgg cggtacttgg ggccatcaat ccggtgcgtc ccctttcgct tccgggtgcc
     3781 ggccgccatc ggcgagtaca ccttgggcga cggcgaaagc ggagttggcg gccggtactc
     3841 cacagtagac agcgcactgc agcagaacct cctttatctc gtcttgcgta agaccgtttc
     3901 gaagggccgc gcggacgtgc atctccagct catcgagctt gcccagcgct accagcgcgg
     3961 tcagggtgat gcagctgcga atcctgcggt ccaggccagg acggatccag atctcgcccc
     4021 aggcatagcg cgtgatgaaa tcctgaaagt cggcagtgaa atccgtttgg cgctctatgg
     4081 cggcgtcgac gtgctcgtca cccaagacct cgcgacgcac cttcatcccg cgctcgtggg
     4141 cagattcacc cgggttcatc tccactccct cacgaccggg atcagatgat cgaggaccgc
     4201 ttgcgtcacg gcctcgggct gctctatgct ggcgaggtgg gcggcgtccg ggataacctc
     4261 gaggcgagcc tcggggatgg agtcgcggat cgacttcgcc ccttccggcg gggcggcgag
     4321 gtcatcagcg ccggcgataa cgagggtagg cgaggagatc gtgccgagcg tgtcccttag
     4381 gtcagtgtcc cggacagcct cgcagcagcc cgcgtagcct tcggcggggg tctccaccag
     4441 catacgtcgg gcccaccctg cgatctcggg ctgtccttcc cgaaaggcag gggtaaacca
     4501 gcggtccacc accgtatccg cgatggcatc gattccgtcg gcgcggaccg tccgggcacg
     4561 cacatcaaac gtgtcgggtg ggaatcgggc cgctgtgcag cacaacacca ggcgctcgac
     4621 gcgctccggt gcctcgccgg ccagccacat ccccaccatc cctccgatag agaggccgca
     4681 gaaggagaag cgctccacct catgccggtc gagaaacacc aaaacgtcgc gtgcgaggtc
     4741 ctcaattctg tatgggcctg gcggtacggg tgaggcgccg tggccacggt ggtcgtagcg
     4801 cacgaggcgg aagcgtttgc tcagctcagg cccctgttcg tcccacatcg cgagcgtcgt
     4861 gcccaaagag ttcgatagca cgagaaccgg cgcatcctcg gggccttcga gcaggtggtg
     4921 tacctcgacg gcggactccg ccttcatgcc tgcacctcct tgcggtagag tcccagggcc
     4981 cggtcgacga acgccccggt cgaaccgaga tagcctgctg ggtcgagcgc atcgtcgatg
     5041 ttctcttcgg agcggacttc cacgagcgcc ggctctgcga gtaactcatc gcgcagggat
     5101 ttcccactct cgagtgtgcg cttggaggta gcctcgacca gatcgtgcgc cttgagccgc
     5161 ccgaggtgtt cagcgactag cgtcgtcacg cgttcggtga gtagcaaacc cccgctggcg
     5221 tcaaggttct ccttcatctt ctcgggatac acctccaagc cctccgtcgc ctcccggacc
     5281 gcggccgcgg tcccgccggt gagggctaag gcgtcggaga gggcttccca ctcggcgtgc
     5341 caggcgcccg ctgcccgctc gtgttctcct gccatcgccg actgcagcgt gcgtgctaga
     5401 tcctgcaccc gtcgcgagca ggcagccgcg gtcacggaga ggatggggtt tcgcttgtgg
     5461 ggcagcgtcg aggaacctcc acgtccggcg cccgcgggct ccgagacctc ccccacctct
     5521 gtctgggcca tcaggatgat gtcgagcgag atcttgccga ggacgcccgc aagaagcgag
     5581 agcgtacctc cgatctgagc gatgcgggac cggtcggtgt gccacggcac cacgggctcg
     5641 gcaaggtcga gctcccgggc aaactccgca aggac
//
LOCUS       DATMVF010000253         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_75285, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000253 DATMVF010000000
VERSION     DATMVF010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75285"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VN178_12780"
     CDS             <1..260
                     /locus_tag="VN178_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81889.1"
                     /translation="GIFIRESYGDVADSDASALGLSFIRDGNSANAFSKLSRYEAGIE
                     RGLYRALHELQRLQAARRADGDAPPPVAIDVDISGVSRDEQ"
     gene            complement(234..674)
                     /locus_tag="VN178_12785"
     CDS             complement(234..674)
                     /locus_tag="VN178_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWS81890.1"
                     /translation="MRELVGAGRVSTVLAQDRDRVAHESASLFYLREEFGQHGRELIA
                     LNDRGDDNPEGQLTDGILDQIARFERLKIAEGSRQRKLRKARGKAPVWDLIFSLLKDP
                     DRSTLIACDSRSKSYFPFCETEVMWVAMPVDKQKPSLLLVSGNT"
     gene            1696..1956
                     /locus_tag="VN178_12790"
     CDS             1696..1956
                     /locus_tag="VN178_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81891.1"
                     /translation="MDGDHHCRECDGTGWVLYRSETEDDGFEEAHRLCPQGHAPRYCM
                     GASDGHLCPRPATRRLGPGYYCEDHIAVIHEDRDIDDPCEAI"
     gene            2016..2099
                     /locus_tag="VN178_12795"
                     /pseudo
     CDS             2016..2099
                     /locus_tag="VN178_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412400.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2147..>3435)
                     /locus_tag="VN178_12800"
     CDS             complement(2147..>3435)
                     /locus_tag="VN178_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWS81892.1"
                     /translation="VRGYRGEAALGGVRPDIEAAVAEAFREEWGRVVATLIRTTGDWD
                     LAEECAQDAFARALETWPRDGVPRSPGAWLTTTARNRAMDRLRRRATGSAKLQEVALL
                     ADPDEPGDDATGESGVRDDRLRLIFTCCHPALPLEARVALTLRTLAGLTTSEIARAFL
                     VPEPTMAKRLVRAKRKIRNAGIPYRVPPAHLLPERTGGVLAVLYLLFNEGYAASAGAD
                     LVRERLSTEAIRLARALAQLMPDEPEALGLLALMLLHDARCSARVDRAGDLVPLEEQD
                     RARWDRDAISEGVDLLDAALRLGRPGPYQVQAAIASCHATAAEASATDWAQIAALYGE
                     LARLVPSPVVELNRAVAVAMAEGPAEGLAMVEELEASGNLAGYHLLPATRADLLRRLG
                     RPAEAATAYHQALELAATDAEHRYLARRLAETTGHP"
BASE COUNT          636 a         1044 c         1121 g          634 t
ORIGIN      
        1 caggcatatt cattcgggaa tcctacgggg acgtggcgga ttcggacgcg tctgcgcttg
       61 gtctgtcctt cataagggac ggcaacagcg cgaacgcctt ctccaagctc tcccgttatg
      121 aggccggcat agagcgcggc ctctacagag cactgcacga actacagcgc ctccaggccg
      181 cccgccgcgc tgatggcgac gctccacccc ctgtggcgat agacgtggac atctcaggtg
      241 tttccagaga cgagcaataa agaaggcttt tgcttgtcga cgggcattgc gacccacatg
      301 acctccgttt cacagaacgg gaagtaactc tttgagcgcg agtcgcaagc gatcagggtc
      361 gagcgatcag ggtctttcaa gagactgaag atgagatccc atactggagc ctttcctcgt
      421 gccttgcgta acttacgctg cctgcttccc tcggctatct tgaggcgctc gaagcgggct
      481 atctggtcga gtataccgtc ggtaagctgg ccttctgggt tgtcgtcgcc gcggtcgtta
      541 agcgctataa gctcgcggcc atgctggccg aactcctcgc gcagatagaa gagagatgca
      601 gactcatgag cgacgcgatc cctatcctga gctagtacag tcgacacgcg tccggcacct
      661 acaagctccc tcacctagcc tatgccgggt ctctccaagc ttgcgacgct ctgtccgaag
      721 atcctggact tccttgagga tctcatagcc ctcacaaggg acgtaggctc tcaaaccatc
      781 gatttattgg tttaggaagc cggtccttgc ttgtctgtgg agacgcgggc atagagggta
      841 gcctgatttg ggtcgtggcc attggtgcta tgtatgatca gtgtcctacg agttcggtgg
      901 ggtctacgcc gagggcttcg gcgagcttgc gaatagtacg gaaatgaggc tctcgatggt
      961 cgcgctcgat gttagcaact gtactaggac taatgcccga cttctgcgcc agctcctctt
     1021 gcgtgagaag ccgtctagtg cggatctctc gcaacctatc tccgatgtag accacggcgg
     1081 acattgtagc ctccttcttc tcgcggatat cgagtaacag tctagtaaca tacaaacaat
     1141 agtaaaacag tagacatacg ctagtaaatg agctagaatg aggacgttgg ataaacagat
     1201 ggctccgcag gagcggcaac tccccggagc ccggcccaca gaaaggttgg catgctagct
     1261 gtggacatga gagagtctag cgcaccgtac gtacacccat ccaccgagaa tgacgacgac
     1321 accctagagg cgcactacac ttgcctagag cgcctgggtc cctgcgcgtg cgtagatggc
     1381 tgggtcttcg tcggctacat agacgagcac ggcgaggaga gagaagcctc ctacccgtgc
     1441 cgcaggtgtg ctgctagccc tggtgggcaa taaccggaga aggtactcat atctgaaccc
     1501 gagaacgggc tacgcttaga acatgcacat tgatgttccg gcagggcgcc caagccgaag
     1561 cggcatggga ttccttcacg agcctagtac ggagagctgg aaggagatta agcttcttct
     1621 actcaactga tatagattgc gaccctcact tcttcgagac tgagtcaaag ctgttcgaag
     1681 ggatgggcag ctagcttgga cggtgatcat cactgtcgtg agtgcgacgg cacgggctgg
     1741 gtgctctatc gctcggagac cgaagatgac gggttcgagg aagcccaccg tctctgtccc
     1801 caaggtcatg cgccgcgcta ctgcatggga gccagcgatg gtcacctttg cccccgcccc
     1861 gcgaccaggc gcttggggcc aggctactac tgcgaggatc acatcgcagt tatccacgag
     1921 gacagggaca ttgacgaccc ttgcgaggcg atctagaggc tctgcggccc aggggcttga
     1981 gggtgtttct aacccaggaa gagggttgtg ggggggcggg gctcgccgaa tacgacgaga
     2041 cggagggtat cgagtgggag tggcaagcca tcgacggggt gatgaccaag gcccctttag
     2101 cctggattat tcatgaacag aacgaaggct gcctgtcggc gcaaggtcag gggtggcccg
     2161 tagtctccgc cagcctgcgg gcgaggtacc ggtgctcggc gtcggtcgcc gccagttcga
     2221 gcgcctggtg gtaggcggtg gccgcctcgg cggggcggcc gaggcggcgg agaaggtcgg
     2281 cgcgcgtggc gggcagcaga tggtagccgg ccagattccc ggacgcttcc aactcctcga
     2341 ccatcgccag cccttccgcg gggccctcgg ccatcgccac ggccaccgcc cggttcagct
     2401 ccacgaccgg ggagggcacc agccgggcga gctcgccgta gagggcggcg atctgggccc
     2461 agtcggtggc ggaggcctcg gccgcggtgg cgtggcacga ggcgatggcc gcctgcacct
     2521 ggtaaggtcc tggcctgcca agccgcagcg cggcgtcgag cagatctacc ccctcggaga
     2581 tggcgtcccg gtcccagcgg gcgcggtcct gttcctctag gggaacgagg tcgcccgcgc
     2641 ggtcgacgcg ggcgctacag cgggcgtcgt ggaggagcat gagcgcgagc agccccagcg
     2701 cctcgggctc gtcgggcatc agttgggcga gggcgcgggc caagcgtatg gcctcggtgc
     2761 tcaaacgctc tcttaccaga tcggcgccgg cgctggcggc gtagccctcg ttgaagagca
     2821 ggtaaaggac ggcaagcaca cctccggttc gttccggcag caggtgggcc gggggcacgc
     2881 ggtacgggat gccggcgttg cggatcttgc gcttggcccg caccaggcgc ttggccatag
     2941 tgggctccgg gaccaggaaa gcgcgagcta tctctgaggt ggtgagtccg gccagcgtcc
     3001 tgagcgtgag ggccacacgg gcctcgagcg gcagcgccgg gtggcagcag gtgaagatca
     3061 ggcgcagccg atcgtctcgg acgccgctct cgccggtcgc gtcgtcgcct ggttcgtccg
     3121 ggtccgccaa caaagctacc tcctgcagct tggcgctccc cgtcgccctg cgcctaagcc
     3181 tgtccatggc ccggttgcgc gccgtggtgg ttagccaagc gccggggctt cgggggacgc
     3241 cgtccctagg ccaggtctca agcgcccgcg cgaaggcgtc ctgagcgcac tcctcggcca
     3301 ggtcccagtc gccggtggtg cggatcaggg tcgccacgac gcgcccccac tcctcgcgga
     3361 acgcttcggc gaccgccgcc tcgatgtccg gcctcactcc tcccagagcg gcctcacctc
     3421 gataaccccg aactt
//
LOCUS       DATMVF010000254         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_132995, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000254 DATMVF010000000
VERSION     DATMVF010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132995"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VN178_12805"
     CDS             <1..1692
                     /locus_tag="VN178_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWS81893.1"
                     /translation="VVPVSQIRSLFPILRNPANRHRAVGFTPEQFHYAFTNTMRRQES
                     DEVYERYHIPAPGSFVWAGPLANFTPGHQDTYVNFRNEERAPLLFIAGSEDNIMPPAV
                     NQSNVKHYRHAKTVTDYKEFAGRSHYTVGQDGWEEVADYALEWAVDHAKHPAGLITPS
                     RSTGGGEQPMETHPQVRPFRVDVPEEDLEDLRRRIEAASWPSKELVEDRSQGVQLATM
                     QALARYWTTDYDWRKAEEKLNALPNFITEIDGVDIHFVRVRSQHENALPLIMTHGWPG
                     SVVELLETVGPLTDPTAHGGTPEDAFHLVLPSLPGYGFSGEPAELGWEAGRTAQAWTE
                     LMRRLGYTRYVAQGGDLGAIVTDVMARQAPEGLVGIHTNFLVTVLADPPPEESEQERA
                     AAAALAEFQATGFAYFLEMTNRPQTIGYALLDSPVALAAWMLDHDTDSYYKISRAFVD
                     GQPVGNLTREHIVDNITLYWLTGTGASAARWYWEFGRFVAAAQASGQAPPEVSVPVGF
                     TTFPGEIWRTSRSWAERSYPNLTYFNEVDRGGHFAAWEEPQLFSEEMRAAFSSLR"
     gene            1732..2394
                     /locus_tag="VN178_12810"
     CDS             1732..2394
                     /locus_tag="VN178_12810"
                     /inference="COORDINATES: protein motif:HMM:NF044569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81894.1"
                     /translation="MPTQTNLFLLEDLEDVGKASELAERDLDALHAVADWINTFVVRP
                     HKDLGRAGPVCPFVPEALERRTHWLAPEQIADRDVPAVVELISGYKRVFLDTQPTDGD
                     DAKYKVIVVVFTDLSADRAQGVFDDVLEQLAVSSYAEDGIVFGPFYEGHEGTAIYNSS
                     FRPFQSPVPFLFVRHGVISDWKFFLDNEDLFNLWARRFGESAVHALAEELRRLPWRSG
                     TR"
     gene            2391..2534
                     /locus_tag="VN178_12815"
     CDS             2391..2534
                     /locus_tag="VN178_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81895.1"
                     /translation="MKFVADDVRTPAVPGSGHWLAEQAPEEMVAAPREFLALYHEGGG
                     GLR"
     gene            2531..>2794
                     /locus_tag="VN178_12820"
     CDS             2531..>2794
                     /locus_tag="VN178_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81896.1"
                     /translation="MSFFQSLFPAHREAGTEVLPSEGPLPPFEGATGWLNSEPLTPEG
                     LRGRVVAVQFWTYTCVNWLRTLPYAREWAAKYHDHGLIVIGVHT"
BASE COUNT          509 a          924 c          871 g          490 t
ORIGIN      
        1 gtcgtccccg tctcccaaat caggtcgctc ttcccgatct taaggaaccc tgccaaccgc
       61 cacagggccg tcggctttac ccccgagcag ttccactacg ccttcaccaa cactatgagg
      121 cggcaggagt ccgacgaggt ctacgagcgc taccacatcc ccgctccggg cagcttcgtg
      181 tgggctgggc ccctcgccaa cttcaccccg ggtcaccagg acacctacgt gaacttcagg
      241 aacgaagagc gggcgccgtt gctgttcata gcggggagcg aggacaacat catgccgcct
      301 gcggtcaacc aatcgaacgt caagcactac cgccacgcca agaccgttac ggactacaag
      361 gagttcgctg gacgctcgca ctacaccgtc gggcaggatg gatgggagga agtagcagac
      421 tatgcgctag agtgggccgt agatcacgcg aagcacccag cggggctgat cacccccagt
      481 cgctctacag gaggaggaga acagcctatg gagacccatc cccaagtccg ccccttccgc
      541 gtcgacgttc cggaggagga cctggaggac ctccgccgac gcatcgaggc ggcgagctgg
      601 ccgagcaagg aactcgtcga agaccgatcc cagggcgtgc agctggcgac gatgcaggcg
      661 ctcgcgcgct actggacgac ggactacgac tggcgcaagg ccgaggagaa gctcaacgcc
      721 ctaccgaact tcatcaccga gatcgacggg gtggacatcc acttcgtccg cgtgcgctcc
      781 cagcacgaga acgcgttgcc gctgatcatg acgcatggct ggcccggctc ggtggtcgag
      841 ctgctggaga ccgtcggccc gctgaccgac ccgaccgccc acgggggcac ccccgaggac
      901 gccttccacc tggtgctgcc gtcgcttccg ggctacggct tctccggcga gcccgccgag
      961 ctcggctggg aggccggccg caccgcgcag gcctggacag aactgatgcg ccgcctcggc
     1021 tacacccgct acgtcgccca gggcggcgat ctgggcgcca tcgtcactga tgtgatggcc
     1081 cgccaggcac ccgaggggct ggtaggcatc cacacgaact ttctcgtgac ggtgctggcc
     1141 gaccccccgc cggaggagtc cgagcaagaa cgcgcggctg cggctgcgct cgctgagttc
     1201 caggccaccg gcttcgccta cttcctggag atgaccaatc ggccgcagac gatcggctac
     1261 gccctgctgg attcacccgt cgccctggcg gcctggatgc tcgaccacga cacggacagc
     1321 tactacaaga tctcccgcgc cttcgtcgac gggcagcccg tgggcaacct cacgcgcgag
     1381 cacatcgtcg acaacatcac gctgtactgg ctgacgggca ccggggcctc ggccgcccgg
     1441 tggtactggg agttcggacg gttcgtggcc gcagcccagg cgtccgggca ggctcctcct
     1501 gaggtctcgg taccggtcgg cttcaccacc ttccctggcg agatctggcg tacttcgcgc
     1561 agctgggccg aaaggagcta ccccaacctc acctacttca acgaagtcga taggggcggt
     1621 cacttcgccg cttgggagga gccgcaactc ttctcggagg agatgcgggc tgcgttcagc
     1681 tcactgcgct aatgacacca ttgttttcgg ctctcttcag aaggaggttc catgccaaca
     1741 cagaccaatc tcttcctcct cgaggatctc gaggacgtcg gaaaggcgag cgagctcgcg
     1801 gaaagggatc tagatgcgct tcacgccgtc gcggactgga tcaacacctt cgtggtcagg
     1861 cctcacaagg atctgggccg cgctgggccc gtctgtccct tcgtgcctga ggccctggaa
     1921 cgtaggacgc attggcttgc tccagagcag atcgccgacc gggacgtgcc ggctgttgtc
     1981 gaacttatta gcggctacaa gagagtgttc ctggacactc aacccactga cggcgacgac
     2041 gcaaagtaca aggtgatcgt cgttgttttc accgacttgt cggctgatcg tgcgcaaggc
     2101 gtctttgacg acgtcctaga gcagcttgca gtttcatcgt atgcggaaga tggaatcgtg
     2161 ttcgggccgt tctacgaagg ccatgagggg actgcgatat acaactcgag ctttcgcccg
     2221 ttccaatcac ccgtgccgtt tctgttcgtg aggcatgggg tcatcagcga ctggaagttc
     2281 ttcctggaca acgaggactt gttcaacttg tgggcacgtc gcttcggaga atccgcagtc
     2341 cacgcccttg ctgaggaact acgccgcttg ccgtggcgct cgggaacacg atgaagttcg
     2401 tggccgacga cgtgcggacc cccgccgtcc ccggcagcgg ccactggctc gccgagcagg
     2461 ctcccgagga gatggtggcg gcgccgaggg agttcctggc cctgtaccac gagggcggag
     2521 gtggcttgcg atgagcttct tccagagcct cttccctgcg catcgggagg ccggaacgga
     2581 agtcttgccc agcgagggcc ccctgccgcc tttcgagggc gccaccggat ggctcaactc
     2641 tgagccactg acgcccgagg ggctccgcgg gcgcgtcgtc gcggtgcagt tctggaccta
     2701 cacctgcgtc aactggctta gaacgctgcc ttatgcgcgt gagtgggccg ccaaatacca
     2761 cgatcacggc ctgatcgtta tcggggttca tact
//
LOCUS       DATMVF010000255         9085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_677148, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000255 DATMVF010000000
VERSION     DATMVF010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677148"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VN178_12825"
     CDS             <1..926
                     /locus_tag="VN178_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HWS81897.1"
                     /translation="ILWFAGASAMAGLLNVVPRYLPRYGMAPEWGRAVRPLVLVYTAI
                     SMIVTVIFAADVGSQAGAYATGILAMMTSAGFAVTLTAWRTGRMLGTVLFGIVTVVFV
                     YALASNVHERPDGITIALFFIGAIIAISLVSRVQRSTELRQERIDVDEMARSFIEEAA
                     QRGEIHLVAHRRRVGTEEEYSRKEREQREDNHIPREEQILFLEVQVDDASEFTDVLEV
                     RGVEVGGHQVLRCVSSVVPNAIAAFLLHLRDTTGKTPHCYFGWTEGNPIVYLIRYILF
                     GEGDTAPVTHEVLREAEPNPERRPAIHVGGR"
     gene            923..1597
                     /locus_tag="VN178_12830"
     CDS             923..1597
                     /locus_tag="VN178_12830"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWS81898.1"
                     /translation="MNRQFVVVGLGRLGISMVGTLDSLGHEVLAIDSRENVIQGLADE
                     LPNVHLVAADATDEAAVRGLDVESFDAAAVMIGENHIEAGILATATLKEVGVPRIVAR
                     ATGGIHARVLEKVGADRVIQPEREIGEQLARTMASPVLLDYVDLGEDEALIEARVPSA
                     WLDKSLSELDLSQKMGLTVLALKPQDGPGSLPHGDTVLSDGDVLVVGGSRAALDRSPL
                     VQPDGR"
     gene            1615..2940
                     /gene="trkA"
                     /locus_tag="VN178_12835"
     CDS             1615..2940
                     /gene="trkA"
                     /locus_tag="VN178_12835"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HWS81899.1"
                     /translation="MIIGAGEVGLNTARMLSHEGHDVVLVEKDERLVESATEELDALV
                     IAGNGANPKLLNEAGIKSSDLLIAASNSDEVNIIACLAAKAQGVSRTVARIHNPDYYE
                     SGDPFAREMLGIDFMIHTEQMAAQEIKDALLVPGAINVDGFAEDTIEVAEVILNEGSE
                     AVGRALREVSLPQGSLIVGVVRGGEALVPKGGTVLEKRDHVLLISGRKQISEAVGALA
                     TDTAPVREVTIYGGGRIGLRLALSLEKAGMSVRVIERDEGRARYVASQLRRGFVLHDE
                     GISRDFMLQEGVDRTDAFVAVTGDDRANLLAAMYARQLGARMTIAGISRGEYAPLADA
                     LGVDLTISPRMLAAEAILRFVRKGEIIDVALLASGAEMIELRVPERCRVAGRPLSEVG
                     FPEGAIVGALLRNGAVIIPTGKEVLRPGDDAVVFTVEAAVEEVEKLFSN"
     gene            2944..4482
                     /locus_tag="VN178_12840"
     CDS             2944..4482
                     /locus_tag="VN178_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HWS81900.1"
                     /translation="MNPRVVIRVNAGVILAIGLALLVPLALSVLYRDGSWASFLLPAA
                     LMVATGIVSFRLSRPRGRAPEYVSNRDVYLSVTLAWTLAALLGGTPFLVEGTFHSLLD
                     SSFEAMSGFTTTGATLLSDIEAQTPSILFWRSMTHWLGGIGIVVLFVAVAPLLGVGTA
                     RLIGAEMSGLTQPRLTPRIADTAKALVIIYLAISAAETVALLLARMPLYDAVVHTFGT
                     VATGGFSHKTASVGFYDSLAIEAIIGFFMVASGVSFSLYYLLYTRRRLDVLLDRELLA
                     YLAIIAAAALFIWGILVLEGDYGDSWGRALRDSAFTASSIITTTGFITADFDQWDAAA
                     EIILILLMFIGGCAGSTAGGIKVIRILIIFRTILQDVFRMIHPRAHTASARRTRGSRG
                     RADRRPRALRGLDRGLRDSDFPRCPPGRADPALFRYRGSGHPERRRPWPGTGRSHGEL
                     RDSRPSGPLRTHRLYAPGEAGDLHRPGTPLPRILASLSVVGAGFKPAPTFNVLVAGRL
                     FCVP"
     gene            complement(4442..5371)
                     /gene="rocF"
                     /locus_tag="VN178_12845"
     CDS             complement(4442..5371)
                     /gene="rocF"
                     /locus_tag="VN178_12845"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HWS81901.1"
                     /translation="MFENGRGPVGIFGVPMDLGQDRRGVDMGPSAIRYARLEARLEDL
                     GHSVTDLGNAVVPIPEVVAPGERVRHLAAVKSVCEEVSERAASIVSEGLFPIFLGGDH
                     AITIGTVSGIASSSPAVRTGVIWVDAHADFNTPATSPSGNIHGMPLATLAGLGHPDLV
                     GIGGPEASVRTEDITIIGLRSVDVEEKKLLREAGVRVYTMKDIDAYGAARVVRSAIKG
                     LTDVDRVHLSLDLDALDPEVAPGVGTPVRGGLTYREAHLLMELVNEAGIISSLDVVEV
                     NPILDIKNGTATLAVELVESLMGRRIIDLPRER"
     gene            complement(5364..6914)
                     /locus_tag="VN178_12850"
     CDS             complement(5364..6914)
                     /locus_tag="VN178_12850"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWS81902.1"
                     /translation="METTGPDRTFAAAAGTPYAAEAAAETYRAGGNAADAAVAAAAAV
                     GVTEPLMSSIGGGGFALVRDPSGGAELIDYYDAMPGKGLPASAFGAGGSPQTMILKYG
                     AGVRSIVGGASSAVPGSLRGWEKLLARHGKLSLKEALAPAVRFAREGFRLCKTSALWF
                     GVAEEVLRLTDETRKHFYKGERVYLEGEEVRFPELADTLEAVAEGGVGIFYEGELGRR
                     ISAYMLQMGGIITEEDLAAYEVVVREPMSVRYGAGAMYTNGPPSAGGPTLAQMLKVVS
                     AYDLATMPAAEYVKVMAGAMKLALRDRETAYLDGSENSRVAERLTGEEYAAEQRRRIF
                     SAFGSPHTTHLSCVDSGGLAVSITASMGYGSGLVVPGTGIPMNNTLGEPELNPRGFHA
                     LAPGERLISNMSPTVVSSEEGGVIALGTPGASRIPTAILQTVINVLDFGMALEDAVLA
                     PRFHAEGDLFAYEAGARTADLDTYERVLSYETPNMFFGGVNAVRRTPDGRFEAAADPR
                     RSGGTDIV"
     gene            8055..8993
                     /locus_tag="VN178_12855"
     CDS             8055..8993
                     /locus_tag="VN178_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81903.1"
                     /translation="MTDVSDLRSSVNNFSGEEKGIRLGSTTRNHDNKVSTYTRISISN
                     CVRDGETSDVAIGILEMFPSAYKEIDPTGTGLTLLVGGKKPTWCKDHKRKLDSKETIE
                     VCEDDDWLPVTRISFGDPVEGVGDHSKAIAKLCEDLFPAEKELFHADKEDLLAAKPTT
                     PSEPQNSFIHYHEFLKHTIQKMYPEFLKVRWENYADPLIILGLLEIAYEEKCGYEDGD
                     LFIDAADRDIRKKTNLSRATIDRRIKYLNRSNYPLTILKKGRLGKRSKYRISPTHKIS
                     KRYRQISPLKKEDASCCEWGSNSAFHSCCAGERYPQ"
BASE COUNT         1745 a         2837 c         2823 g         1680 t
ORIGIN      
        1 ccatcctgtg gttcgccggc gcctcggcca tggcggggct gcttaacgtg gtgccccgct
       61 acctcccccg ctacggcatg gcgcccgagt ggggcagggc cgtcaggccg ctggtgctcg
      121 tctacacggc gatctccatg atagtcacgg tgatcttcgc cgccgacgtg ggctcgcagg
      181 cgggggccta cgcgacgggt attctggcga tgatgacctc ggcgggtttc gccgtcacgc
      241 tgaccgcctg gcgcaccggg cgcatgctgg gcacggtcct cttcgggata gtaaccgtcg
      301 tcttcgtcta cgcgctggcc tccaacgtcc acgaacgtcc cgatggcatc accatagcgc
      361 ttttctttat aggggcgatc atcgctatct ccctggtgtc ccgcgtccag cgctcgaccg
      421 agctcaggca ggagcggata gacgtggacg agatggccag gagtttcatc gaggaggcgg
      481 cccagagggg tgagatacac ctcgtcgccc accgtcgccg cgtcggcacc gaggaggagt
      541 actccagaaa ggagagggaa cagcgcgagg acaaccacat accgcgggag gaacagatcc
      601 tgttcctgga ggtacaggtc gacgacgcct cggagttcac ggacgtgctc gaggtcaggg
      661 gcgtcgaggt cggtggtcac caggtgctgc gttgcgtaag ctccgtcgtc cccaacgcga
      721 tcgcggcctt cctcctgcac ctgcgcgaca cgacggggaa gacgccgcac tgctacttcg
      781 gatggacgga gggcaacccc atcgtctacc ttatccgcta catcctcttc ggtgaaggcg
      841 acaccgcgcc cgtcacccac gaggttctgc gcgaggcgga gccgaacccg gaacgccgtc
      901 cggcgatcca cgtgggcggc cgatgaaccg gcagttcgtc gtggtcggcc tgggtcgtct
      961 cggtatctcc atggtcggca ccctcgactc cttggggcac gaagtactcg ccatagacag
     1021 cagggagaac gtgatacagg gcctcgcgga cgagttgccg aacgtccacc tcgtggccgc
     1081 tgatgcgacg gacgaggccg ccgtgagggg tctggacgtc gaaagcttcg acgccgcagc
     1141 cgtcatgatc ggcgagaacc acatcgaggc cggcatcctc gcgacggcca ccttgaaaga
     1201 ggtcggagta cccaggatcg tggcgcgggc cacgggcggt atccatgcca gggttctcga
     1261 aaaggtggga gccgacaggg tgatacagcc cgaacgagag ataggtgagc aactcgccag
     1321 gaccatggct tctccggtcc tgctcgacta cgtcgacctc ggagaggacg aggccctgat
     1381 cgaagcccgg gtcccgagtg cgtggctgga taaatccctc tccgagctcg acctctcgca
     1441 gaagatgggc ctcacggtcc tggccctcaa gccacaagac ggaccagggt ccctgcccca
     1501 cggtgacaca gtactaagtg acggcgacgt gctcgtcgtc gggggatcca gggcggccct
     1561 ggacagatcc ccactggtcc agcccgacgg tcggtaggag gcgctttgca cacggtgata
     1621 atcggggcag gagaggtcgg gctcaacacg gcccgtatgc tctcgcatga gggccacgac
     1681 gtcgtgctcg tcgaaaagga tgagaggctc gtggagagcg caaccgaaga gctcgacgcc
     1741 ctggtgatag caggcaacgg cgccaacccc aagctgctca acgaggccgg catcaagagc
     1801 tccgacctgc tcattgcggc gagtaattca gacgaggtca acatcatcgc ttgcctggcg
     1861 gcgaaggccc agggagtctc ccgtaccgtg gcgcgcatac acaaccccga ttactacgaa
     1921 tcgggagacc ctttcgccag ggagatgctg gggatcgact tcatgatcca caccgagcag
     1981 atggccgcgc aggagatcaa ggacgcgctg ctggtgcccg gcgcgatcaa cgtggacggc
     2041 ttcgccgagg acaccatcga ggtggccgag gtgatcctga acgagggctc ggaggccgtg
     2101 ggacgcgcgc tgcgcgaagt aagtttgccc cagggcagcc tcatagtcgg cgtcgtgagg
     2161 ggcggagagg ccctcgtgcc gaaaggcgga acggtgctgg agaagaggga ccacgtactt
     2221 ctgatcagcg gccggaagca gatctccgag gccgtgggcg ccctggccac cgacacggcg
     2281 ccggtgcggg aggtgaccat ctacggcgga gggcgcatcg gcctcaggct ggccctctca
     2341 ctggagaagg ccggcatgtc ggtgagggtc atagagaggg acgagggcag ggcgcgctac
     2401 gtggcatccc aactaaggag agggttcgtg ctccacgacg aggggatctc ccgcgacttc
     2461 atgctccagg agggggtgga ccgtaccgac gccttcgtcg ccgtaacggg ggacgacagg
     2521 gccaaccttc tggccgccat gtacgcccgc cagttggggg cccgcatgac catcgccggg
     2581 ataagccgcg gcgagtacgc gccgctcgcg gacgccctcg gcgtcgacct gacgatctca
     2641 ccgaggatgc tggctgccga agccattctc cgctttgtgc gcaagggcga gatcatcgac
     2701 gtggccctgc tggcgagcgg ggccgagatg atcgagctca gggtccccga gcgatgcagg
     2761 gtcgccggcc ggccgctctc tgaggtaggg ttccccgaag gcgccatcgt gggggcccta
     2821 ctgcgcaacg gggcggttat catcccgacg ggtaaggaag ttctcaggcc cggcgacgac
     2881 gccgtggtgt tcaccgtcga ggccgccgtg gaagaagttg agaagctctt ctccaactag
     2941 cctttgaacc cgagggtcgt aatcagggtg aacgccggtg tgatcctggc gatcgggctg
     3001 gcgctcctgg tccccctggc gctctcggtg ctctaccgcg atggctcgtg ggcgagcttc
     3061 ctgcttcccg ccgcgctgat ggtcgcaact ggtatcgtaa gcttccgcct ctccaggcct
     3121 cggggccgag cacccgagta cgtgtcgaac agggacgtct acctctccgt gacgctggcc
     3181 tggacgctgg ccgccctgct cggcggcacg ccgttcctcg tcgagggcac cttccacagc
     3241 cttctggact cgagcttcga ggcgatgagc gggtttacta ccacgggggc caccctcctc
     3301 tcggacatcg aggcccagac gccatccatc ctgttctggc gaagcatgac ccactggctc
     3361 ggggggatag ggatcgtggt cctcttcgtc gcggtcgcgc cgctcctcgg ggtcgggact
     3421 gcacgcctga tcggagccga gatgtcgggg ctcacgcagc cccgcctgac gccccgcatc
     3481 gcggacacgg ccaaagctct cgtcatcatc tacctcgcca tctcggccgc cgagaccgtc
     3541 gcattgttac tagcgaggat gccactctac gacgccgtcg tccacacgtt cggcacggtc
     3601 gcgacggggg gcttcagcca caagaccgca tccgtcggct tctacgactc gctcgccata
     3661 gaggcgatca tcggtttctt tatggtggcc tcgggcgtct cgttctcgct ctactacctc
     3721 ctctacacca ggcgccggct cgacgtcctg ctcgacagag aactgcttgc ttacctcgca
     3781 atcatcgccg ctgcagccct ctttatctgg gggatcctcg tcctggaagg cgactacggg
     3841 gactcctggg gcagggcact cagggactcg gcgttcacgg cctcgagcat catcaccacg
     3901 acgggattca taacggcgga tttcgaccag tgggatgcgg cagccgagat cattctcatc
     3961 ctgctcatgt tcatcggggg atgcgccggg tcgacggcgg gcgggataaa ggtgatccgc
     4021 atcctcatca tcttccgcac catcctccag gacgtcttca ggatgatcca cccgcgcgct
     4081 cacaccgctt cagctaggcg gacgcgtggt tccagaggcc gtgcggatcg ccgtcctcgg
     4141 gctcttcgcg gcctggatcg gggtcttcgg gatagcgact ttcctcgttg ccctccagga
     4201 agagctgacc ccgctctctt ccgctaccgc ggtagcggcc accctgaacg acgtcggccc
     4261 tggcctggga caggtcggag ccacggagag ttacgagata gtcgaccctc tgggccgctt
     4321 cgtactcacc gtctgtatgc tcctggggag gctggagatc ttcaccgtcc tggcactcct
     4381 ctcccccgca ttctggcgtc gctgagcgta gtaggggcgg gtttcaaacc cgcccctaca
     4441 ttcaacgttc tcgtggcagg tcgattattc tgcgtcccat gaggctctcg acgagctcga
     4501 ctgcgagtgt ggcggtgccg ttcttgatat ccaggatagg gttgacttcg accacgtcga
     4561 gcgaagagat tatgcctgct tcgttgacca gctccatcag gaggtgcgcc tccctgtagg
     4621 ttagcccccc gcgcacgggt gtgccgacgc caggggcgac ttcggggtcg agggcgtcga
     4681 ggtcgagtga gaggtgcacc ctgtcgacgt ccgtgaggcc tttgattgcg cttcgcacga
     4741 cgcgcgccgc gccgtaggca tcgatgtcct tcatggtgta gaccctcacg ccggcctcgc
     4801 gcagcagctt cttctcctcc acatccacgg agcggaggcc gatgatggtt atgtcctcgg
     4861 tcctgacgct ggcctcagga ccgccgatcc caacgaggtc ggggtggccg agaccggcga
     4921 gggtcgccag cggcatgccg tggatgttgc cggagggcga cgtcgcagga gtgttgaagt
     4981 ccgcgtgggc gtcgacccag ataacacccg tccggactgc gggcgaggag ctcgcaatcc
     5041 cggatacggt cccgatagtg atggcgtgat ccccaccgag gaagatcggg aacaaaccct
     5101 cggagactat ggacgcggcc cgttcggaga cctcttcaca gacgctcttt acggcggcca
     5161 gatgcctgac cctttccccc ggagcgacga cctcgggtat aggcactaca gcgttcccga
     5221 ggtcggtaac gctgtgcccg aggtcttcga ggcgtgcttc gaggcgggca taacggatgg
     5281 cgcttggtcc catatccacg cccctccggt cctggccgag atccatgggc acaccgaaga
     5341 tccccaccgg cccacgtccg ttctcaaaca atgtcagtcc ctcccgaacg tcggggatcg
     5401 gccgccgcct cgaagcgacc gtccggtgtg cgtcttacgg cgttgacccc gccgaagaac
     5461 atattgggtg tctcatagct cagcactcgt tcgtaggtgt cgagatccgc tgtgcgggcg
     5521 ccggcttcgt aagcgaagag atctccctcc gcgtggaagc gcggggcgag aacggcgtcc
     5581 tccagcgcca tcccgaaatc cagaacgttt atgaccgtct gcagtatggc cgtagggatg
     5641 cgcgaagcgc ccggcgttcc cagcgcgatg acaccgccct cctccgagga gacgacggtc
     5701 gggctcatgt tggagatcaa acgctccccc ggcgcgagcg cgtggaaccc cctggggttg
     5761 agctcgggct cgccgagcgt gttgttcatg gggatgcccg taccgggcac gacgagtcct
     5821 gacccgtagc ccatgctggc cgtaatcgag acggccaggc ccccgctatc gacgcacgag
     5881 aggtgtgtgg tgtgcggaga gccgaaggcg gagaagatcc tgcgtcgctg ctccgccgcg
     5941 tactcctctc ccgtgagccg ctccgcgacc cggctgttct ccgaaccgtc gaggtaggcg
     6001 gtctcgcgat ccctgagagc cagcttcatc gccccggcca tcaccttcac gtattcggca
     6061 gccggcatcg tagccaggtc gtaggcggag acgactttga gcatctgggc cagtgtcggg
     6121 ccgccggcgg aaggcgggcc gttggtgtac atggccccgg cgccgtacct gacgctcatc
     6181 ggttccctga cgacgacctc gtacgcggcg aggtcctctt cggttatgat gccgcccatc
     6241 tggagcatgt aggcggagat cctgcgcccc agctccccct cgtagaagat ccccaccccg
     6301 ccctctgcga ccgcctccag ggtgtcggcg agctcgggga accgcacctc ttcaccctcc
     6361 aggtacaccc gctccccttt gtagaagtgt ttgcgggtct cgtcggtgag cctcagcacc
     6421 tcctcggcga ccccgaacca cagggccgag gtcttgcaca acctgaaccc ttcgcgcgcg
     6481 aacctgaccg caggagcaag cgcctccttc aggctcagct taccgtggcg cgccaggagc
     6541 ttctcccatc cccgaaggga acctggtacg gcggacgacg cccccccgac gatggagcgc
     6601 accccggccc cgtacttgag gatcatcgtc tgcgggctgc cgccggcccc gaaggccgaa
     6661 gccggcagac ccttgccggg catcgcgtcg tagtaatcga tgagctcggc tccgccggaa
     6721 ggatctctga cgagagcgaa cccgccgccc ccgatcgaac tcatcagcgg ctcggtaacg
     6781 ccgacggcgg ccgccgcggc tacggccgca tcagcggcgt tgccccctgc cctgtacgtc
     6841 tcggcagcgg cctcggccgc atacggcgta cccgcagcgg cggcgaaggt gcggtcgggg
     6901 cccgtagtct ccataattgg ctatgatacg ccagcagaca cgctagcgga aagggttcgc
     6961 atcttgagcg aggcgagaag cggcggtagg aggttcaatt cggccactgt gccgcataga
     7021 atagccagat tgtctacccc tatccttcac atactgcgca acactctatt gttcacaaat
     7081 agagtcgaca atatgttgtc atcttctttg tagaccaacg gttggtacac ggttggtcta
     7141 cagacactcc atgagtacag ctccgacctg cattacttgt tgccgcagtt ccaaaggccc
     7201 ccaagtttgt ccccggcact gacgaggcac tagggtagct ctcagacgtt tcaggagccg
     7261 ttctgagcgg tttggcggtt gtgtcctcgc caggcattga atgttaactc ccagttggac
     7321 tcgggacatc cctttcttca ctacttttat agagaatagg gaagccccta tctatgggga
     7381 ggtgtcacgc gaaggggagt ggaaggagag taaggatgag tagccttgga gacacggaga
     7441 ctggagaaaa ctcaggaagt ccagggcacc cagcacagca caggaacatg tcacggatgg
     7501 gtagcccaca caccccgtga gacgagcgag tatgccttct aagggcctgc gagggggtct
     7561 tgagtagtcc tgtggcacgg gagggtacac actatccgga tcagcaaagc cgacagtcac
     7621 atgtgcgata aagtgcgata aagaccgtcg ttggcatctg gcgagtatag tcattaggtg
     7681 ccatgtcgat ggaccgctag aggaccggat atacgccgga taatccactc tttgtgttac
     7741 tatagagggt agctaatctc catagaaatc cccgacaaga atcgggatct ctcccataca
     7801 ggaggaaata taattgacgc cgaagcagtt acctctcgtc tttcatttcc agatgcccga
     7861 taaagcctgc aacaattata attcatgcat atcactgacg ggtaggacgg agttagtaac
     7921 aaatgcaaca ctaccccttc accctctgat ctcttcagca gttatacgcc tcccgagaag
     7981 aggagcaagc tcagcgagga caagctcgcc gatggatgac cgaccggtcc atggagatag
     8041 cggggtgatt accgttgact gacgtttccg acctccgttc ctctgtcaat aacttctccg
     8101 gggaggagaa gggtatacgg ttgggcagca ccacaagaaa ccatgacaac aaggtttcaa
     8161 cctacaccag gatctccatc agcaactgcg tacgagacgg ggaaaccagc gatgtagcga
     8221 taggcatcct ggagatgttc ccttccgcct acaaggagat agatcccacc gggaccggcc
     8281 ttaccctatt ggtaggaggg aaaaagccta cctggtgtaa ggatcacaag cgtaagcttg
     8341 atagcaaaga gacgatagag gtgtgcgaag atgatgattg gctccccgtt accagaatca
     8401 gctttggtga ccctgtagag ggggtaggag accattccaa ggcgatagca aagctgtgtg
     8461 aggatctctt ccctgccgag aaggaactct tccatgccga taaagaagat ctgttggccg
     8521 ccaagcctac tacacctagc gaaccacaga actcctttat tcactaccat gagttcctca
     8581 aacataccat tcagaagatg tatccagagt tcctgaaggt cagatgggag aactatgccg
     8641 atcctcttat catcctcggt cttctggaga tagcctacga agagaagtgt ggttatgagg
     8701 atggcgatct cttcatagat gccgccgaca gggacatcag gaaaaagacc aacctctcca
     8761 gagccacaat agacagaagg atcaaatacc ttaacaggtc caactaccct ctcactatcc
     8821 tcaagaaggg taggttgggg aagcgctcca agtaccggat atctcctaca cacaagatat
     8881 ccaagaggta cagacagatc tcccctctaa agaaggagga cgcgtcttgt tgtgaatggg
     8941 gttctaacag cgcctttcat agttgctgcg ccggggagcg gtatccgcag tgagcaaagt
     9001 accctttcgc gtcttcggcg ctcacggccg ccaatgctag acccatcgcc tcgatgagga
     9061 catccttggt gcgggccccg atctt
//
LOCUS       DATMVF010000256        11874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_445790, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000256 DATMVF010000000
VERSION     DATMVF010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445790"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VN178_12860"
     CDS             <1..375
                     /locus_tag="VN178_12860"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81904.1"
                     /translation="FISGREILDRATEELGDLEPIEKEDPAAPADEFRFDGAPGTFGV
                     ITPVSEPFCARCSRLRLTADGKIHPCLLTDLDVPVKDAIRSADPIPAIHKVLWETARI
                     KPAAGLTLPEESRHRTMSQIGG"
     gene            complement(421..1152)
                     /locus_tag="VN178_12865"
     CDS             complement(421..1152)
                     /locus_tag="VN178_12865"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HWS81905.1"
                     /translation="MTPLEIIRQAQSGTLIDEGGHVVTLELLPGLSHTELQDFVGRVP
                     CHTQPEIMELLGACRGFYGTVDKVEFTGRDLMFEFGAAFPYGLPIAADGCGNFWVVDL
                     HPDTAGWGPVYFACHDAPVILYQADSLEQFLDELFRMFEPPHRSLIDDVHEDRLARVW
                     QTNPGVLAHEQCLRSEYPILSIFARELDDESFQIIDLRLAKPGDGFSWGRYGPNTRIR
                     RFGTHPVFAYKKPKSFISRLLELAV"
     gene            complement(1264..1593)
                     /locus_tag="VN178_12870"
     CDS             complement(1264..1593)
                     /locus_tag="VN178_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HWS81906.1"
                     /translation="MQSIPTLHGVAIPFASSTETKPGIEIERSVTQEPPYKVILHNDD
                     YTPMEHVVAVLQKVIPRMSVKRAVDIMMEAHTKGKAVATKCHKELAELYQDGLNNEGL
                     ISTIEPD"
     gene            complement(1670..2845)
                     /locus_tag="VN178_12875"
     CDS             complement(1670..2845)
                     /locus_tag="VN178_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HWS81907.1"
                     /translation="MAAQVLSGRTAIVHDLALEDYGFGGDHPFDPLRVRLALELCEAL
                     GLLEDYPFVSPGPATDEDLTTVHSLTYVRLVQEAGRGAAELSDLLDYGLGTPDNPIFP
                     EMHEACARVVGGVLGACRLVMAGEADHAMCISGGLHHAMRTRASGFCVYNDAGVAISR
                     LKEEHPDVRIAYVDTDAHHGDGVQWMFYGDPDVLTVSMHESGRYLFPGSGGVDEKGRG
                     GGVGYSVNVPLEPFTDDESWISCFEVVVPAALRAFDPDLILSQNGCDGHALDPLTHLS
                     ATTRFYEHVPHRIHDLAHGLCGGRWVATGGGGYDIWRVVPRAWTSLWAAVSHQELPER
                     VPEPWLSKWSTKSPVRLPPFMRDDPEDYPQGPRTAKIAERNLRTVEEVVERVLPMIQ"
     gene            complement(2827..3477)
                     /locus_tag="VN178_12880"
     CDS             complement(2827..3477)
                     /locus_tag="VN178_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HWS81908.1"
                     /translation="MLLVEDSMTRDIVTLAPDETVGTALALCRERRIRHLPVLSEGRL
                     VGIVSDRDLRSATPAFGDHERAAALQEIIVEDVMATEVVSVLPDDPIEQAANTMRERR
                     IGCLPVVEGGELVGIITSSDVMSALVYLVGANEPGSRMEISVPDRPGSLAGAAGVFGM
                     CGINIVSAAMGPIREPMEEGAIRERIVVFRVDTIDTSEAVGYLEEAGYSVLWPPRS"
     gene            3554..3928
                     /locus_tag="VN178_12885"
     CDS             3554..3928
                     /locus_tag="VN178_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81909.1"
                     /translation="MPEYLNGLLADSAAALVHESFTGVSAPWWWERRLGGGIEVCQEF
                     DPEVASREISAKTGHEVSEVRRAIAEELGLEDPEPVVLTFEVAGETETRRVAEMLAER
                     SSEPEGLAAGLYRRIEEIVRHK"
     gene            complement(3932..4261)
                     /locus_tag="VN178_12890"
     CDS             complement(3932..4261)
                     /locus_tag="VN178_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWS81910.1"
                     /translation="METFGYENITPGALKSRLDAGERPILLDVREPWEFELARIEGSE
                     LIPMSQLEARFTELDPGAETVVICHHGNRSSYVAAALRRVGFERVFNLEGGLDAYSDV
                     DQSVPRY"
     gene            complement(4266..4616)
                     /locus_tag="VN178_12895"
     CDS             complement(4266..4616)
                     /locus_tag="VN178_12895"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HWS81911.1"
                     /translation="MVLGRLLDSIGYNREQGGGAVVDHLVFLTVREEASPEEVEDLLS
                     SIRALRDEVPSTVDLSVGEDFSGRSGGYTHAIFARFEDAAGLREYMEHPAHLAVVEKL
                     DATTTGRIVADYEY"
     gene            complement(4683..5651)
                     /locus_tag="VN178_12900"
     CDS             complement(4683..5651)
                     /locus_tag="VN178_12900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS81912.1"
                     /translation="MNYRTLGRTGLEVSEVGYGAWGIAGDAWLGAKDEESLKALNRAV
                     DLGLNFVDTALAYGEGHSERLVGKLVQERDETIHVATKVPPKNRLWPAPEGIAPEEVF
                     PGDYIRECTEQSLSNLGLDTIDVQQFHVWQDDWVGKGDWLEAVEDLKREGKIRAFGVS
                     INDHQASNAVRLIETGTVDTVQVIYNVFDQSPEDRLLPACQEHDIGVIVRVPFDEGSL
                     TGRITPDTSFEEGDFRNHYFRGERKQEVYDRVRAIISELGASEDEIAEIALRYILSHP
                     AVSTVIPGMRSVRNVERNMAVADGQGLPEDQVQVLKNHRWDRNFYA"
     gene            5763..7529
                     /locus_tag="VN178_12905"
     CDS             5763..7529
                     /locus_tag="VN178_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HWS81913.1"
                     /translation="MRILDERREALVERERALLERLIAFLEDFGAPPEDVGLVRRAHQ
                     DLEDLFLLVIVGEFNSGKSAFVNALLGADVSEEGVTPTTDRITLLRYADEPVEKGRPD
                     GVREKGFPNEFLREIAIVDTPGTNAIIRHHEELSRGFVPRSDLVLFVTSAERPLTESE
                     RGYLELIRDWGKKILLVINKADLLADESNLEEVRSFVDGGIRSALGLTPPIFFVSALL
                     ARKAKAATNALERDALMKASGFAELESYVTELLDEGGRVRLKMESPLGVVDELNRRYG
                     GAVDERLTLLREDFRTAENVESQLELYKEDLGRDFEARMAEIENIVLRMNERGDAWLE
                     ENIRFANIPELFRQQKVSERFEREVVADTEELVDERVDELIDWMVDRNLKQWRMVVEY
                     VNRRRQAEYDERLIGEVGDGFEYNRGRLIQSVGKNAQDVVQRYDRERESEKLAHSIQG
                     AVTQAAALEVGAVGIGGVVVALATTRFLDVTGIIAAAIIAGYGLFILPSRRRKAREEF
                     RGKTDSLRERLGEVVRRQFEAESNRSVERMREAIAPYTRFVRSEHARMTGAGEELAAL
                     EAETRALKDEISAPGVGPKTSL"
     gene            7601..7903
                     /locus_tag="VN178_12910"
     CDS             7601..7903
                     /locus_tag="VN178_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81914.1"
                     /translation="MADRETKERKLVALMVNDLGPGFGKREMADLQKEVGALSDPELD
                     ETLAGRLDLPHCVGEDELEQALLHVEERPGGDQERFTGAPDMSHEPRPEERGGPSE"
     gene            8134..10032
                     /locus_tag="VN178_12915"
     CDS             8134..10032
                     /locus_tag="VN178_12915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWS81915.1"
                     /translation="MRALAVIAVLLYHADLAWIPGGFLGVEVFFVISGYLITALLLAE
                     WRQRGRIDLKTFWLRRARRLLPALYVLLVVSLAFAVVFLPGEVAGLRGDVLAAFGYVT
                     NWYLIFGQESYFEAVGRPSLLQHLWSLAVEEQFYLIWPPILALGLCFGATRLRQRRLL
                     TVVLLGAGASAVAMALLYTPGVDPSRIYYGTDTRATGLLFGAALAFVWSPGVRYRPSE
                     ARRALAGRGRFRRRWGWTAPLLLDILGFVALGGLVWLCHHLGEFQPFLYRGGFALVGL
                     ATTATIMAGVHPYTLVGARLLGSAPLRWVGVRSYGIYLWHWPVFMVTRPELDVPFDGP
                     YLLALRLAATVILADLSYRYIETPIRRGTLGRAWRTLREARGPLRWRLRLQWAGALVP
                     ILASCALLGLAVARAEPPEKPSYLASMKSVHTVEQTHPDPSTAGTNGLRKDERADHQN
                     SGENGEAPMTQAQRALYNGRTGPSEFSGTVSAIGDSVMLGAVRRLQKDIYGLTVIDAE
                     VGLQIYAATDTLRYRRAAGQLGDVVIVHLGNNGTFTKGQFDRIMRTLSSVDRVVFVNV
                     SIPRAWEAPNNQVIAEGVERYPNAVLVDWHSASADRPELFYKDGYHLRPKGQRIYAGL
                     ISAHLPEE"
     gene            complement(10162..10641)
                     /locus_tag="VN178_12920"
     CDS             complement(10162..10641)
                     /locus_tag="VN178_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWS81916.1"
                     /translation="MAMRPYRGFYDMQSEMNRMFDEVFGGLSRRSGRQHGDEPTRWAP
                     VLDVLQEDGDIVVRAELPGVKQEDVDITLHDGVLTISGERKAEEQREGSGYYVRERRY
                     GSFRRSMTLPQGIDESEISARFDGGVLEVRVSGAAAVREPKRIQIEAGDEGEGASGS"
     gene            complement(10729..10938)
                     /locus_tag="VN178_12925"
     CDS             complement(10729..10938)
                     /locus_tag="VN178_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81917.1"
                     /translation="MAGKKHGRYYAQELAVDENTNVMDQIQGALDEGDHHDWHLVGVS
                     DVMAEHGVILFWDTERPSFGISHRQ"
     gene            complement(11180..>11874)
                     /locus_tag="VN178_12930"
     CDS             complement(11180..>11874)
                     /locus_tag="VN178_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81918.1"
                     /translation="IEKGGTPITMRTITKGVFCSAGRSGLSVPALVTLASSIAALVLA
                     LSATTAQSAEGHDGKNIPLSGGKTLLKIDKGTAAALSDAGVKVEATGAAEGPTARHPY
                     FTFPIVGGKVDKDPPSGRIVHSGGLRFSADSNSVVVKRFVIDLGRGVLTAKVAGTGQR
                     IALLRLGAPDGVKIGDGRLVLKGVNVRLTAQAASALNEALDTDLFAGGLLIGEATVIA
                     KYGDDDGRHDDD"
BASE COUNT         2102 a         3842 c         3720 g         2210 t
ORIGIN      
        1 tttatctccg ggcgtgagat cctggaccgc gccacagagg agctgggtga tctcgaaccc
       61 atcgaaaagg aagatccagc agcccccgct gacgagttcc gcttcgatgg cgcccccggc
      121 actttcggcg tcataacgcc ggtctcggaa cccttctgtg ctcgttgcag caggctcagg
      181 ctcacggcgg acggcaagat ccacccttgc cttctcaccg acctcgacgt tcccgtaaag
      241 gacgcaatcc gctctgcgga ccccataccg gcgatccaca aagtgctttg ggagacggcg
      301 cgaataaagc cggcggccgg ccttaccctc cccgaggaat cccgccatcg caccatgagc
      361 cagatcgggg gctaaagggc gaaccttcga tggccgcgaa cgtggcccag ccgctcgctg
      421 tcaaaccgca agttcgagaa gccgggagat gaagctcttc ggcttcttgt aagcgaacac
      481 gggatgtgtg ccgaagcgtc tgatccgggt gttgggcccg tagcggcccc aggagaagcc
      541 atcgcccggt ttcgccagac gaaggtcgat tatctggaag ctttcgtcat ctagctcgcg
      601 cgcgaagatc gagaggattg ggtactcgga tcggagacac tgctcgtggg ctaggacccc
      661 agggttcgtc tgccacacgc gggccaggcg gtcctcgtga acatcgtcga taaggctccg
      721 gtgcggtggt tcgaacatcc ggaacagttc atccaggaac tgttccaacg aatcggcctg
      781 gtagaggatc actggcgcgt cgtggcacgc gaagtagacg ggaccccacc ccgcggtgtc
      841 cgggtgtaga tctacgaccc agaaattacc gcagccgtct gccgcgatcg gaagtccgta
      901 agggaatgcc gccccgaact cgaacatgag atccctaccc gtgaattcga ccttgtcgac
      961 cgttccgtag aaacctctac aggcgcctag caactccatg atctctggtt gagtgtgaca
     1021 cggcacccgg cctacgaagt cctgcagttc tgtgtgggag aggccgggaa gaagctccaa
     1081 cgtgacgacg tggccacctt cgtcgatgag cgttcccgac tgcgcttgac ggatgatctc
     1141 gagaggagtc acgagtggtc tcccggctaa acacctttgt aagatcatac cggcgcccga
     1201 tgcggttccc aacgtcctgt tgactggcgc agcctgaaac ccgcccctac gccgcaaacc
     1261 gcgtcagtca ggttctatgg tggagatgag gccttcgttg ttgagcccgt cctggtagag
     1321 ctcggccagc tctttgtggc acttggttgc gacggctttg cccttggtgt gggcttccat
     1381 catgatgtct acggccctct tcacggacat gcgcgggatc accttttgca acacggcgac
     1441 gacgtgctcc atcggagtgt agtcgtcgtt gtgcagtatg actttgtaag gtggctcctg
     1501 ggtgacgctg cgctctatct ctatccccgg cttcgtctcg gtcgaagacg cgaaagggat
     1561 cgctacgcca tgcaacgttg gtatgctttg cactttcatc tccggatcgc cgaactgctc
     1621 acctagggca aaataccgcg acgacgcagg attcgcaacc ctgtccgtcc tactgaatca
     1681 tgggtagcac tctttcgacg acttcttcga cggtgcgaag gttacgttcc gctatcttgg
     1741 cggtacgcgg tccctgcgga taatcctcgg gatcgtcgcg catgaaaggc ggtagtctga
     1801 caggactctt cgtgctccat ttcgagagcc agggttcagg tactctctcg ggcagttcct
     1861 ggtgggagac ggccgcccag agcgaggtcc aggcgcgcgg gacgacgcgc cagatgtcgt
     1921 agcccccgcc gcccgttgcc acccagcgtc ccccgcagag tccgtgggcc aggtcgtgta
     1981 tccgatgggg cacatgctcg tagaagcggg tcgttgcgga gaggtgagta agcgggtcga
     2041 gggcatggcc gtcgcagccg ttctgggaca ggatcaggtc ggggtcgaag gcgcgaaggg
     2101 cagcggggac cacgacctcg aagcaggaga tccaggactc atcgtcggta aacggttcga
     2161 gcggcacatt cacggagtag ccgactccgc cgcctctgcc cttctcatcg accccgccgc
     2221 tgccgggaaa cagataacgg cccgactcgt gcatcgagac ggtaaggaca tccgggtcgc
     2281 cgtagaacat ccactgcaca ccgtcgccgt ggtgggcgtc ggtgtcgacg taggcgatgc
     2341 ggacgtcagg atgctcctct ttgaggcgag agatcgcgac cccggcgtcg ttgtagacgc
     2401 agaagccgct ggcccttgtc ctcatggcgt ggtgcagtcc gcccgagatg cacatggcgt
     2461 ggtccgcctc ccctgccatc acgagccggc acgcgccgag cacaccccct accacgcggg
     2521 cgcaggcctc gtgcatctcg gggaagatgg gattgtccgg ggtcccgaga ccgtagtcca
     2581 gcaggtcgga gagctcagcc gctccccgtc cggcctcctg gaccagacgc acgtaggtga
     2641 ggctgtggac ggtcgtcagg tcttcgtcgg tcgccggtcc gggggagacg aaggggtagt
     2701 cttccagcag cccgagggct tcgcagagct cgagtgcgag ccgcacgcgc agagggtcga
     2761 aaggatggtc gccgccgaag ccgtagtcct ccagggccag gtcgtggact atggcggtcc
     2821 ggccgctcaa gacctgggcg gccacaggac cgagtagcct gcctcctcga ggtatccgac
     2881 cgcctcgctg gtgtcgatgg tgtccaccct gaaaacgacg atgcgttccc tgatcgctcc
     2941 ttcctccatg ggttccctta tcgggcccat agccgcgctg acgatgttga tgccgcacat
     3001 cccgaagacg ccggccgcgc cggccagaga ccctggcctg tcggggaccg agatctccat
     3061 gcggctcccc ggctcgttgg cgccgaccag gtagacgagc gcgctcatca cgtcagagga
     3121 ggtgatgatc ccgaccagtt ccccgccctc cacgacgggt aggcagccga tcctacgctc
     3181 tcgcatcgtg ttcgcggcct gctctatggg atcgtcgggg agaacgctga ccacttcggt
     3241 ggccatcacg tcctcgacaa taatctcctg cagcgcggcg gccctttcgt ggtccccgaa
     3301 cgcgggggtc gccgaacgca ggtccctgtc cgagacgatc ccgacgagcc tcccttccga
     3361 aaggacggga aggtgcctga tgcgcctctc cctgcacagg gctagagcag tacccaccgt
     3421 ctcatccggc gcgagcgtta cgatgtcacg ggtcatcgag tcttctacga gaagcatcgg
     3481 ataactcctt ccctcgtcgc attgtacagt agcggatcgt cgcgggtaag cgtgcgcctg
     3541 gtataaatca cccatgcccg aatatctcaa tggactcctc gccgacagcg cagcggccct
     3601 ggtgcatgag agctttacgg gcgtaagcgc cccgtggtgg tgggagcggc gcctgggagg
     3661 aggcatcgag gtctgccagg aattcgaccc cgaagtcgcg agccgtgaga tctcggcgaa
     3721 gacaggccac gaggtctccg aagtccgccg ggccatcgcc gaagaactcg gcctcgaaga
     3781 ccccgagccc gtggtcctca ccttcgaggt tgctggcgag accgagaccc gccgcgtcgc
     3841 cgagatgctg gccgaacgtt cctcggaacc cgagggcctc gccgcaggcc tctaccgacg
     3901 gatcgaggag atcgtcaggc acaagtagga gtcaatacct cgggacggac tggtccacat
     3961 cggagtaggc gtcgaggccg ccttccaggt tgaagactct ctcgaagccg acccgccgga
     4021 gcgccgcggc gacgtaggag ctgcggttgc cgtggtggca gatcacgacg gtctcagcgc
     4081 caggatccag ctcggtgaaa cgcgcctcca gctgggacat ggggataagc tccgaaccct
     4141 ctatgcgggc gagctcgaac tcccagggct cgcgcacatc gagcagaatg ggacgttctc
     4201 cggcatcgag tctgctcttc agagcgcccg gcgttatgtt ctcgtagccg aaggtctcca
     4261 tgtcgctagt actcgtagtc ggcgacgatg cggcctgtcg tcgtagcgtc gagcttctcg
     4321 acgacggcca ggtgcgctgg atgctccatg tactcccgaa gaccggcagc gtcctcgaac
     4381 ctggcgaaga tggcgtgcgt ataccctcca gagcgtcccg agaagtcttc gcccacggag
     4441 aggtcgacgg tgctgggcac ctcgtctctc aaagcgcgga tagatgagag gagatcctcg
     4501 acttcttcag gggaagcctc ctcacgaacg gttagaaata cgagatggtc taccacagca
     4561 ccgccccctt gctccctgtt gtatccgatg gagtctagca gacgacccag taccatccac
     4621 aactagctgc ggcgttcggt cccgcctgac ttgtagcgtc gatgcaggcg tcccttctgt
     4681 ttctaggcgt agaagttgcg gtcccagcgg tggtttttga gtacctgcac ctgatcctcg
     4741 gggagtccct ggccgtcggc gacggccatg ttacgctcga cgttgcggac ggagcgcatg
     4801 cccgggatga cggtcgagac ggcagggtgg ctcaggatgt aacggagtgc tatctcggct
     4861 atctcgtcct cgctcgcgcc gagctcggag atgatggccc gcacgcggtc gtagacctcc
     4921 tgtttgcgtt caccgcggaa gtagtggttg cggaagtcgc cctcttcgaa ggaggtgtcg
     4981 ggggtgatcc tgcccgtcaa tgagccctcg tcgaagggaa ccctgactat cacgccgatg
     5041 tcgtgttcct ggcaggccgg aaggagccgg tcctccgggc tctggtcgaa gacgttgtag
     5101 atcacctgca ccgtatctac ggtgcccgtc tcgatgagcc tcaccgcgtt cgacgcctgg
     5161 tggtcgttta tggagacgcc gaaagcgcgg atcttgcctt ctcttttgag gtcctctacc
     5221 gcctccagcc aatccccttt gccgacccag tcgtcctgcc agacatggaa ctgttgcacg
     5281 tcgatggtgt ccagcccgag gttggagagg ctctgctccg tgcactccct gatgtagtct
     5341 ccggggaaca cctcctcggg agcgatgcct tcgggagcgg gccagagcct gttcttcggc
     5401 ggaaccttgg tggcgacgtg gatggtctcg tcccgctcct ggaccaactt ccccacgagc
     5461 cgctcgctgt gtccctcccc gtacgccagg gccgtgtcta caaagttgag accgaggtcc
     5521 accgccctgt tcagggcctt cagcgactcc tcgtccttcg cgccgagcca ggcgtcgccg
     5581 gcgatgcccc aggccccgta gcctacctcc gatacttcca ggcccgtcct gccgagcgtt
     5641 ctgtagttca cgcagcctcc atgtgtagtg cttgccggaa agtcctcttc ttgttaccac
     5701 gagcgggtca tcgcgaaaca gcgaggcgta tataatgctt gcatgcagag ggaggcataa
     5761 gggtgcggat actcgacgaa cgccgcgagg cgctggtaga gcgggagcgg gcgctactgg
     5821 agagactgat agccttcctc gaggacttcg gggcgccgcc ggaggacgtt ggccttgtca
     5881 ggcgggcgca ccaggacctg gaagatttgt tcttgctcgt catcgtaggg gagttcaact
     5941 cgggcaagtc cgcgttcgtc aacgcgttgc tcggggccga cgtctccgag gagggcgtca
     6001 cgccgacgac ggaccgtatc accttactcc gttacgccga cgagcccgtg gagaaaggac
     6061 gtcctgacgg ggtacgggag aaggggtttc ccaacgagtt cctgcgtgag atcgccatcg
     6121 tcgacacgcc tgggaccaac gcgatcatcc ggcatcatga ggaactctcc aggggcttcg
     6181 tgccgaggag cgacctggtg ttgttcgtca cctccgccga gcggcctctc accgagagcg
     6241 agcgagggta cctcgagctc atccgagact gggggaagaa gatcctgctc gtcatcaaca
     6301 aggccgatct tctggccgac gaatcgaacc tcgaggaggt tcgctccttt gtggacggcg
     6361 gcatacgttc ggccctcggc ctgacgcccc cgatcttctt tgtctcggcc ctccttgctc
     6421 gcaaggccaa ggctgcgacc aatgccctgg agcgcgacgc cctcatgaag gcgagcggct
     6481 tcgcggagct cgaaagctac gtcacggaat tgctcgatga aggtggcaga gtccggctga
     6541 agatggagag cccgctcggg gtagtcgacg agctaaaccg gcgctacggg ggagcggtgg
     6601 acgagcgact gaccctcctc agagaggatt tcaggaccgc ggagaacgtc gagtcgcagc
     6661 tcgagctata caaggaggac ctgggcaggg acttcgaggc gcggatggcc gagatcgaga
     6721 acatagtctt gaggatgaac gagcggggcg acgcctggct ggaggagaac atccgcttcg
     6781 ccaacatccc cgagctcttc cgccagcaga aggtgagcga gcgcttcgag cgggaggtcg
     6841 tcgcggacac cgaggaactc gtggacgagc gggtggacga gctcatagac tggatggtcg
     6901 acaggaacct caagcaatgg cgcatggtcg tggagtacgt caaccgccgc cgccaggccg
     6961 agtacgacga gcgcttgatc ggggaggtcg gcgacggttt cgagtacaac cgcggccggc
     7021 tcatccagtc cgtcggtaag aacgcccagg acgtcgtgca gcgctacgac agggagcgcg
     7081 agtccgagaa gctggcgcac tccatccagg gcgccgtcac ccaggccgcc gcgctcgagg
     7141 tcggggccgt gggtatcggg ggcgtcgtgg tcgcgctcgc aacgacccgc tttctcgacg
     7201 ttacggggat catcgccgcc gcgataatcg ccggctacgg cctgttcatc ctcccgagcc
     7261 gcaggcgcaa ggcccgcgaa gagttccgtg ggaagacaga ctccctacgc gaacgcctgg
     7321 gcgaggtagt cagacgccag ttcgaagccg agtcgaaccg ctccgtcgag cgtatgcggg
     7381 aggccatagc cccctacacc cgtttcgtcc ggtccgagca cgcccgcatg accggcgccg
     7441 gagaagaact cgcagccctc gaagccgaga cccgtgcttt gaaagatgag atctccgccc
     7501 ccggggtcgg gccgaagaca tccctctaag cgtcaccccg tggcacggca ccgaagatcc
     7561 gttgtagact cgaacgccgg aagctctcca ggaggcgaag atggctgacc gtgagaccaa
     7621 ggaacgcaag ctcgtagccc tgatggtaaa cgacctcgga cccggattcg ggaagaggga
     7681 gatggccgat ctccagaaag aggtgggcgc cctctccgac cccgagctcg acgagacgct
     7741 cgcgggaagg ttggatctcc cccattgcgt gggcgaagat gagctggagc aggccctgtt
     7801 acatgtcgag gaacgcccag gcggtgatca ggaacgcttc accggcgccc ccgatatgag
     7861 ccacgagccg cggcccgaag agcgaggagg tccgagcgaa tagcactagg tatagtactt
     7921 acagtacttt tctatccgcc tggcctgcat ggcactttga gactgtgcta ggatcacctt
     7981 cctaccttag gaggacggga gcctacggaa catacgctgc aacaaccgca gacggccgat
     8041 cccgcgaccg gcggcaaggg accatacaga cgcgtcggcg caacgccagg cacccctgat
     8101 gtaaggctgc cttacagtcc gggcctggac ggcctgcgcg cactcgccgt catcgccgta
     8161 ctcctctacc acgcggatct ggcctggatc cccggcggct tcctgggggt cgaagtcttc
     8221 ttcgtgatca gcggctatct cataacggcg ctgttgctcg cggagtggcg ccagcggggt
     8281 cgcatcgacc tgaagacctt ctggctgcgg cgcgcgagga ggctcctccc cgcgctctac
     8341 gtgttgctcg tcgtcagcct ggccttcgcc gtggtcttcc tgcccggtga ggtggcgggg
     8401 ctgcgcggcg acgtgctggc cgctttcgga tacgtgacca actggtatct gatcttcggg
     8461 caggagtctt acttcgaggc cgtcgggcgt ccttcgctgc tacaacatct gtggtcactc
     8521 gccgtcgaag agcagttcta cctgatctgg cctccgattc tcgccctcgg gctatgtttc
     8581 ggggcgacgc gcctcaggca acggcgcttg ctgaccgttg ttctccttgg cgccggggca
     8641 tcggcggtgg ctatggcgct cctgtacacg cccggcgtgg acccgtccag gatctactac
     8701 ggaacagaca cgcgcgctac ggggttgctc ttcggagccg cgctcgcctt tgtgtggtcg
     8761 cccggagtca ggtaccgtcc ttcggaagcc cggcgagcct tggccgggcg gggacggttc
     8821 aggcgccgct ggggatggac cgccccactc cttctcgaca tccttggctt cgtcgcgctg
     8881 ggtggactcg tctggctgtg tcaccacctc ggtgagttcc aacccttcct gtaccggggc
     8941 ggcttcgcgc tggtcgggct cgcgacgacg gcgacgatca tggccggcgt tcacccgtac
     9001 acgctcgtag gagcgcgcct tctagggtcg gctccgctgc gctgggtagg ggtgcgctct
     9061 tacggcatct acctctggca ctggccggtg ttcatggtta cccgccccga actggacgtg
     9121 ccgttcgacg ggccgtacct gctcgcgctg cgcctggccg cgacggtcat cctggcggac
     9181 ctctcctacc gctacatcga gacgccgata cgccgcggca ccctcggacg ggcctggagg
     9241 acgctccgcg aagcgcgggg accgctgcgc tggcggctcc ggctccagtg ggccggcgcc
     9301 ctcgtgccca ttctggcctc ctgcgcgctg ctgggcctag ccgttgcccg ggccgaaccg
     9361 ccggagaaac cttcctacct cgcctccatg aagtccgtac ataccgtgga acaaacgcac
     9421 cccgatccct ccaccgccgg gacaaacggt ctccggaagg acgaacgagc ggatcaccag
     9481 aactccgggg agaacggcga ggccccgatg acccaggcac agagggccct gtacaacgga
     9541 cgaacaggtc cttccgagtt tagcggcaca gtgagcgcca tcggcgactc ggtgatgctc
     9601 ggcgcagtca gaagactgca gaaggacata tacgggctca cggtcatcga cgcggaggtg
     9661 ggcctacaaa tctatgccgc taccgacacg ctgagatatc gtcgcgccgc ggggcaactc
     9721 ggagacgtgg tgatcgtaca cctcggaaac aacggtacgt tcaccaaagg acaattcgac
     9781 cggatcatgc ggaccctctc cagcgtagat agggtcgtat tcgtcaacgt ttcgatcccc
     9841 cgcgcctggg aggctccgaa caaccaggtg atcgcagaag gggtggagag ataccccaac
     9901 gccgtgctcg tcgactggca ctcggcgagc gcagatcgcc ccgagctctt ctacaaggac
     9961 ggctaccatc tccgccccaa aggccagcgg atctacgccg gcctgatctc ggcgcacctc
    10021 cccgaagagt agaagcgatc ggcagggggt cggtgacccc ctgccgatcg cgctgtcagc
    10081 tatcagccgt cagcaaagac aagaagctga gggctgagcg ctgagggctg acagcctgca
    10141 taggggcaaa gcccctttgc attaactgcc cgaggcaccc tcgccttcgt caccggcctc
    10201 gatctggatg cgcttcggct cccgtacggc cgccgcgccg gagactctca cctcgagcac
    10261 accgccgtcg aaacgggcac tgatctcgct ctcatctata ccctgcggca acgtcatgct
    10321 ccttctgaac gaaccataac gccgctcccg cacgtaatag ccagaaccct cacgctgctc
    10381 ctcggccttg cgttccccgg agatggtcag caccccatcg tgcaacgtga tgtctacgtc
    10441 ctcctgcttg acaccgggca actccgccct gacgacgatg tcgccgtctt cttgcagtac
    10501 gtcgagcacc ggcgcccacc gggtcggctc gtccccgtgc tgcctcccac tcctgcggga
    10561 gaggccgccg aacacctcgt cgaacatccg gttcatctca ctctgcatgt cgtagaaacc
    10621 gcggtaaggt ctcatggcca tctcgactac ctccttacgc tctccggctc cctaccagag
    10681 ccaccccttt cggctatcct attggccgaa ctgccatgcc cggagtggct actgtctgtg
    10741 gctgataccg aagctgggtc tctcggtgtc ccagaacagg attacgccgt gctccgccat
    10801 cacgtccgac acaccgacga gatgccagtc gtggtggtcg ccctcgtcga gggcgccctg
    10861 aatctggtcc atgacgttcg tgttctcgtc caccgcaagc tcttgagcgt agtatcggcc
    10921 gtgctttttg cctgccatcc tatgctcccc tttccttagc tcctggatca cagtataggc
    10981 acgaggccgg ccatacagca acctcatctg gatagcccgc tacggctcta ttcctgcaga
    11041 aagactccgc tagcccaggc tcctggggcc tttacgtatg agggcggggc tatccccgga
    11101 gggaagcgcc ccgcccttct agtcgccggc cggttttgcg cggcgctacg gccacgcaaa
    11161 acctaccccg agctatcctt tagtcgtcgt cgtggcgtcc gtcgtcgtcg ccgtacttgg
    11221 cgatgaccgt ggcctctcct atcaacaggc cacctgcgaa caggtcggtg tcgagcgcct
    11281 cgttcagcgc actggccgcc tgggcggtca gcctcacgtt gacgcccttt agcacgagcc
    11341 gcccgtcgcc gatcttcacg ccatcgggag cgcccagtct aagcaacgct atgcgctgcc
    11401 ccgttccggc taccttggcg gtcaggacgc ctcggcccag gtcgatcacg aagcgcttca
    11461 ccaccacgct gttggagtct gcgctgaagc ggagcccccc ggaatgcacg atccggccgc
    11521 ttggcggatc cttgtccacc ttgccaccga ctatggggaa ggtgaagtac gggtgcctgg
    11581 cggtaggtcc ttcggccgcc ccggtcgcct ccactttgac gccagcatcc gagagggctg
    11641 cagccgtgcc tttgtcgatc ttcagcaggg tcttcccgcc gcttagcggg atattcttgc
    11701 catcgtgccc ctcggcgctt tgggcggtgg ttgccgagag cgccaggacg agcgccgcga
    11761 tcgagcttgc gagcgtcacc agcgccggga cactcaggcc tgagcggcct gcggagcaga
    11821 atacgccctt cgttatggtc ctcatggtga tgggtgtgcc tcctttttcg atgg
//
LOCUS       DATMVF010000257         8317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_76814, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000257 DATMVF010000000
VERSION     DATMVF010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76814"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..567)
                     /locus_tag="VN178_12935"
     CDS             complement(172..567)
                     /locus_tag="VN178_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS81919.1"
                     /translation="MDETAEAHSHCVLDPGCASRKVFEAVSGRWGPLVIMALKGQTKR
                     HGELRRELEGISQKMLIQTLRNLEHNGLVEREVYPVVPPKVEYSLTPLGASLQPLLEA
                     ICQWSEQHLEEVEEARQDAGQAAFGCGVA"
     gene            776..1225
                     /locus_tag="VN178_12940"
     CDS             776..1225
                     /locus_tag="VN178_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWS81920.1"
                     /translation="MARRMTEKERQEFLAEPRIGVLSVPRGGDRPPHTTPVWYAYEPG
                     GSITFFTGTQGRKSRKAELVRKAGVLSLTVQREDFPYGYVTVEGTVVGEDRPPPAERA
                     FAVANRYLPEGAAQGFVRAEIGHPAGEFVLFTVRPDRWLTFDFSDEE"
     gene            1296..1868
                     /locus_tag="VN178_12945"
     CDS             1296..1868
                     /locus_tag="VN178_12945"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HWS81921.1"
                     /translation="MGADPASASLQHASYRETKRYLAEHGPGEDRPTGHQFNKSEFFQ
                     RPLPVEAISALVDNLEEGRVRGQSRELDFTPWAGAYNQVRAEASAFAHRDELFVLLQL
                     VVIEPDATAAEREAARHWLARSWASVHPWGSGVVYPNWPDPDLEDWAHAYHGTNLERL
                     VRVKKKYDPDNFFRFDQSLPSQRQGSGAPA"
     gene            1974..2465
                     /locus_tag="VN178_12950"
     CDS             1974..2465
                     /locus_tag="VN178_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWS81922.1"
                     /translation="MSVTEELLQNNAAYAESFEKGDLPLPPAKAVAVVACMDARLDVH
                     KILGLGEGDAHVIRNAGGVITDDEIRSLTISQRLLGTQEIILIHHTDCGMLTFSDDEL
                     KAQIHEDVGLKPHFSMESFSDLEEDVRQSIARIQASPFILHKESVRGFIYEVETGRLR
                     EVD"
     gene            complement(2683..3291)
                     /locus_tag="VN178_12955"
     CDS             complement(2683..3291)
                     /locus_tag="VN178_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS81923.1"
                     /translation="MFIEMAEKRKYEMKKRAERQRETRRRIVEATVELHRTRGPAGTT
                     ISEIARRAGVNRLTVYNHFPDVTDLLRACSRSWTERHPAPDPTPWAWISDPQVRFRKA
                     LTELYGFYARTEPMRANVLRDAQTMPELAALLEGSVVPYLEALRDLLAEGWEVRGKEK
                     GRLLATLALALDFHTWRSLERKSGLSRNEAVETMLEAVRSSV"
     gene            3388..3954
                     /locus_tag="VN178_12960"
     CDS             3388..3954
                     /locus_tag="VN178_12960"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS81924.1"
                     /translation="MSELVNRRARRVLSPDEGETVWLRKLGIRFMIGGEETHGTFALV
                     EHPIGPRALAAPMHTHEREDEYTYVLEGEIGVQIGEEVLVARPGDLIFKPRGVPHAFW
                     NAADEPARALEIISPAGFERYFAELAPLFPPANRGPLDVEAVGAVREKYGLEMDMGSI
                     PVLAERHGLTIDAPPPSESLTPPSGARE"
     gene            complement(3975..4103)
                     /locus_tag="VN178_12965"
     CDS             complement(3975..4103)
                     /locus_tag="VN178_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81925.1"
                     /translation="MQPTEALKPSGEYCEVCGTEMYGLHCKLICPNCGYRRDCSDP"
     gene            complement(4168..4563)
                     /locus_tag="VN178_12970"
     CDS             complement(4168..4563)
                     /locus_tag="VN178_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81926.1"
                     /translation="MYTLLLILILLGALISAVTAFRALGAYLSFRRTRAAFQEEVTDE
                     VARLSARTDELEISLSALDARATELPIQISELQQSLTTLQVLTRALGTSLRQVQKVLS
                     YSSLKTLSADRIGRMIQTASSQRNREPSS"
     gene            4636..4709
                     /locus_tag="VN178_12975"
     tRNA            4636..4709
                     /locus_tag="VN178_12975"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4670..4672,aa:Met,seq:cat)
     gene            4897..5316
                     /locus_tag="VN178_12980"
     CDS             4897..5316
                     /locus_tag="VN178_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HWS81927.1"
                     /translation="MSEGSGGSPLKGEMGSTTISSTVVSQIAGIAAQEVENAQMGGGA
                     SAAVGGILQSVTGSVTGGSSGGNYTRGVSVEVGEEEAAVDLNMTVDYGQSIPRVTEAA
                     RRNVINRVENLTGLRVTEVNILVNDVQVPEQQPALEQ"
     gene            5485..6771
                     /gene="tilS"
                     /locus_tag="VN178_12985"
     CDS             5485..6771
                     /gene="tilS"
                     /locus_tag="VN178_12985"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWS81928.1"
                     /translation="MDLRRPLALVSGGPDSVALLRVVVSLGGEPVVLHVDHGLRGEES
                     HEDAEFVGALCRNLNVRHEVRWISLDGSSNLQERARDERYRLAEVVAAGMGLGVIATG
                     HTADDVAETVLMNLARGTGLRGLAGIPPVRGKVQRPLIGCTRSEILDYLREIDQPYRT
                     DPTNLTGKYARNRVRLEVLPVLEELYPAATGNIARAASLIREDLELLEELAAGTLQLR
                     GEEVVLPLSELMNLRPALRRHAVRLAYATVVPDNAPLPSNLIEAVLELLEGGEGTRTL
                     DLPGGVVASGRPGEELAFYKGRRSMVSGREEIKAGEAVVFGGWRITAREISGYDPSDA
                     ARPEVAYLDAGNGPYQVRMAREGDIIRPLGLGGTKNVFRAMMDRKVPSDLRRGMPVVV
                     DGRDEVAWIVLGELDERYKVDARTEKILRLEVARIS"
     gene            6774..7325
                     /gene="hpt"
                     /locus_tag="VN178_12990"
     CDS             6774..7325
                     /gene="hpt"
                     /locus_tag="VN178_12990"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWS81929.1"
                     /translation="MSSMMPDVKEVLITSREIEEKVREIGARITEDYWGENPLLIGVL
                     RGAVVVMSDLMRNIDLKCELDFMDISSYGSGTSSSGVVRILKDLEEDITDRHVLIVED
                     IIDTGLTLSYLRRSLLARKPASLEICALLSKPSRRRTDLDVRYLGFEVPDEFVVGYGL
                     DYAGAYRNLPDICILKPEVFATG"
     gene            7403..>8317
                     /locus_tag="VN178_12995"
     CDS             7403..>8317
                     /locus_tag="VN178_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS81930.1"
                     /translation="MGRILRSGGLLYFVILIVLAVVLVNMLSKGNPDVAELNSQQWNQ
                     AVQNRTFDTSLEKTNENYLTIYDEDQTVRGQLNTDSGSTKEFEYSYTQMTDVASQLDK
                     ADVPYSVDPQNTGFWLTLLGTLAPILLIVLFFILFMSSMQGGGNRVMSFGKSRARRMT
                     KDQPKVTFSDVAGADEAVQELTEIKEFLEAPQKFQKLGARIPKGALLVGPPGTGKTLL
                     ARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRQ
                     RGAGLGGGHDEREQTLNQLLVEMDGFDSK"
BASE COUNT         1584 a         2511 c         2712 g         1510 t
ORIGIN      
        1 gtcatggata acaacattgc aaacgaggcg gtgccgcggg ccggacgcag ggagtgggta
       61 gggctggcgg tcctggcgct gcccaccctg ctgttgtcgc tggatttgag cgtgctggga
      121 tggtggcgga gtgttcgctg gagcgagtcc ccggaacgtg ctcggtgact actacgccac
      181 gccgcacccg aacgccgcct gacctgcgtc ctggcgtgcc tcctccacct cctcgagatg
      241 ctgctcagac cactggcaga tggcctcgag caagggctgt agggacgccc caaggggggt
      301 caacgagtac tcgaccttcg gcgggaccac ggggtagacc tcccgctcga cgaggccatt
      361 gtgctctaag ttcctcaggg tctggatgag catcttctgc gagatcccct ctagctcccg
      421 cctgagttcc ccgtggcgct ttgtctgccc cttgagcgcc atgatcacga gcggccccca
      481 cctgcccgag accgcctcga agaccttcct cgaagcgcac ccggggtcca acacgcagtg
      541 cgagtgggct tcagccgttt cgtccacggt cccccttggt taccttaggg taagtatagc
      601 acccacgagt aagtacttgt gagttcgcgc acctaaagtt accattactt ggaggtattg
      661 cggagggcgg ttttggcggc caagggagaa ggtaaaccat ggatttcgtc ttgatcccgg
      721 cgccgggatg ggtacccagg tctgggagcc ggtggcctgc gaaaaggagt aagagatggc
      781 ccgtcggatg accgagaaag agcgccagga gttccttgcc gagccgcgaa tcggggtgct
      841 gagcgtgccc agaggcggtg acaggccgcc gcacacaacg ccggtctggt acgcctacga
      901 gccgggtgga agcatcacct tcttcacggg gacgcagggc cgcaagtccc gcaaggccga
      961 gctggttcgg aaagcgggcg tccttagcct taccgtccag cgcgaggatt tcccttacgg
     1021 ttacgtcacc gtcgagggga ccgtcgtcgg agaagaccgc ccaccgcccg ccgagcgggc
     1081 gttcgccgtc gcgaaccggt acctgccgga gggggcggcc cagggcttcg tccgggcgga
     1141 gatcgggcac ccggcgggtg agttcgtgct gttcacggtc cgccccgacc gctggctgac
     1201 cttcgatttc tcggacgagg agtagagatg cacagccttg gcaaccacga ggtggagagc
     1261 ccgatgaacc gtccgagggt agtgtcggcg cccgggtggg agctgacccg gcgtcggcgt
     1321 ctctccaaca cgcgtcctac cgggagacca agcggtacct cgccgagcac ggacccggcg
     1381 aagatcggcc gaccgggcac cagttcaaca agtccgagtt cttccagcga ccgctgcccg
     1441 tcgaagccat atcggcgctg gtggataacc tcgaggaagg acgggttcgg ggccagtcgc
     1501 gtgagctgga cttcacgccg tgggccggcg cctacaacca ggtgcgcgcc gaggcctcgg
     1561 ctttcgccca ccgcgacgag ctcttcgtgc tcctgcaact ggtcgtcatc gaacccgacg
     1621 cgaccgccgc cgagagggag gccgctcggc attggctggc acggtcgtgg gcgtccgtgc
     1681 atccgtgggg ctcgggggtt gtctacccga actggccaga ccccgacctc gaagattggg
     1741 cgcatgctta ccacgggacc aacctcgagc ggctggtgcg cgtcaagaag aagtacgacc
     1801 ccgacaactt cttccgcttc gaccagtcgc tcccgagcca gcgccaggga agcggcgccc
     1861 cggcgtgatg gcgcacgaca aaccgtcccg ggtcggtacg ctcgtccgac tcgggaaagt
     1921 cgttcggcga gtacgctatc gtaaacatcc ttcaatgggt ggtgtcgaag gcgatgagcg
     1981 taacggagga gttgctccag aacaacgcag cctacgccga gtctttcgag aagggcgatc
     2041 taccgctgcc ccccgccaaa gcagttgcgg tggtcgcctg catggacgcc cgcctcgacg
     2101 tgcacaagat cttgggcctc ggggagggtg atgcacacgt catccgcaac gcaggaggcg
     2161 tcattacaga cgatgagatc cgctcgctca ccatctccca gcgtctgctt ggcacccaag
     2221 agatcatcct catccaccac accgactgcg ggatgctcac cttctcagac gatgagctca
     2281 aagctcagat ccacgaagat gtaggcctga agccacattt ctcgatggag agcttctcgg
     2341 atctggagga ggatgtgcgc cagtctatcg ctcgaatcca ggcgagcccg ttcatcttgc
     2401 acaaggagag cgtacgcggc ttcatctacg aggtagagac tggaagattg cgcgaggtcg
     2461 actagcgtat tctcgggcca accaccatcc aacctacgtt ttcatacagt ctcgtgcagg
     2521 gggtctaaca gcgggtagta gaaggattga gttcctgcaa cccgcgccgt aagccctcaa
     2581 ctataagcct tcagctcttt gttcttggct gaacgcttat agcttatcgc tgatagctcg
     2641 tcgtgcataa gggttgaccc tttgcacgac gctgtaaggg cttcacacgg aagagcgcac
     2701 cgcctcgagc atggtctcca cggcttcatt tctgctcaag ccgctctttc gctcgagcga
     2761 acgccaggtg tgaaagtcga gtgccaaagc gagcgtcgcg agtaggcgcc ctttctcttt
     2821 accgcgaacc tcccagccct cggcgagcag gtcgcgcaac gcttcgaggt agggcacgac
     2881 cgaaccctcg agcagagcag cgagctcggg catagtctgg gcgtcgcgca ggacgttcgc
     2941 cctcatcggc tcggtgcggg cgtagaagcc gtagagctca gtcaatgctt tccgaaacct
     3001 tacttgcggg tcgctgatcc atgcccaggg cgtcggatcc ggcgccggat gccgctcggt
     3061 ccagcttctc gaacatgccc tgagaagatc ggtaacgtca gggaagtggt tgtagacggt
     3121 caacctgttc accccagcgc gccgggctat ctcgcttatt gtggtgccgg cgggtccccg
     3181 cgtgcggtgt agctcgaccg tggcctctac gatccggcgc cgcgtctccc gttggcgctc
     3241 ggcccgcttc ttcatctcgt acttgcgttt ttcggccatt tcaataaaca ctatatcaaa
     3301 tgatcttgac aaagggatca gataggtgta tggtgccgac aattcggtaa acatagtgtt
     3361 tattgaactt aggagaggag atcgttcgtg agcgaattgg taaaccggcg agcgcgcagg
     3421 gtgctctcgc cggatgaggg cgagacggtg tggctccgta agcttggcat aaggttcatg
     3481 atcggaggag aggagaccca cggtaccttc gcgctcgtcg agcacccgat cgggccgcga
     3541 gccctcgctg cgccgatgca cacccatgag cgtgaggatg agtacaccta tgtgctggag
     3601 ggagagatcg gcgtccagat aggtgaggag gtactcgttg ccaggcccgg ggatctgatc
     3661 ttcaagccaa gaggtgtgcc acacgccttc tggaacgcgg ctgatgagcc ggcacgcgcg
     3721 ctcgagatca tctcccctgc gggcttcgag cgctacttcg cggagcttgc tcctctgttc
     3781 cctcctgcca acaggggtcc actcgacgtg gaagcggtag gcgctgtgag ggagaagtac
     3841 ggcctcgaga tggatatggg ctctatcccc gtgctcgccg aacgccacgg ccttacgatc
     3901 gacgcgccac cgccttccga gagccttacc ccgccaagcg gtgcacggga gtagcgaaac
     3961 gctcgtgcac tgatctacgg gtcggagcag tcgcggcggt agccgcagtt gggacagatg
     4021 agcttgcaat ggaggccgta catctctgtg ccacagacct cacagtactc tcccgaaggt
     4081 ttcagggcct ccgtgggttg cacctgttgc gcgctctcct cgtccatgtt ccgtagtata
     4141 cggcgcgtct ttgacaccgg cgacgcgcta gctggagggc tctctgttcc tctgggagga
     4201 agcggtctgg atcatcctgc cgatgcggtc ggcgctgagc gtctttagcg agctgtagga
     4261 gaggaccttc tgcacctggc gcagggaggt gcccagcgcc ctcgtcagaa cctgcagcgt
     4321 cgtcagactc tgctgcaact cggagatctg gataggaagc tccgtggcgc gggcgtcgag
     4381 ggcggagagg ctgatctcca actcgtcggt tcgtgcagag aggcgggcga cctcgtccgt
     4441 aacctcctcc tggaaggccg cgcgggtgcg ccgaaagctc aggtaagccc cgagcgcccg
     4501 gaaggccgtc accgcagaga tcagggcccc cagaagtatc aggatcagaa gaagcgtata
     4561 catgtccagg gcaaccgtac cacggcgctc cccgccttta caaactctag gccggtgtta
     4621 aaatcccacc tgcgacgcgg ggtggagcag tctggtagct cgtcgggctc ataacccgaa
     4681 ggtcgcaggt tcaaatcctg cccccgctac ttctccgagg ccccgccccg cggggttaat
     4741 ctttcctgga tatgtcccga gagtctgcac ctgacatgcg cgtgagaacg tgcggagttt
     4801 aggatctggg acggcagggt aggcagctta gttaactgtg cgtggtgacg cacagaactc
     4861 acatctggtg gaaagaatgg atcggaaagg acgggcatga gcgaaggaag cggcggcagc
     4921 ccactgaagg gtgagatggg cagcactacg atcagcagca cggtggtctc ccagatagcg
     4981 ggcatcgcgg cgcaggaggt agagaacgcg cagatgggcg gcggcgcctc cgcggccgtc
     5041 ggtgggatcc tgcagagcgt gaccggtagc gtcacgggcg gctctagcgg ggggaactac
     5101 accaggggcg tgagcgtcga ggtcggcgag gaagaggccg ccgtggacct gaacatgacc
     5161 gtagactacg gccagtcgat accccgtgtc acagaggccg cgcgcagaaa tgtgatcaac
     5221 cgcgtagaga acctcaccgg cctgcgtgtg accgaggtga acatactggt caacgatgtc
     5281 caggtccccg aacagcagcc ggcgctggag caataggagc agacggagta gccggccaga
     5341 gttagaggac aactcctgaa tacggaggac cggggcgcca cgctccggtc ctttcttttg
     5401 cgagcccttg tacttctcta aactatctgc atgggatccg tgagtttccc ggataaggtg
     5461 catgggactg cggagcgtta cggcatggat ctccgtcgcc cgctcgcgct ggtctccggc
     5521 ggccccgact cggtggcgct gctgcgggta gtcgtctcgc tcggcggcga acccgtggtg
     5581 ctgcacgtgg accacggtct gcgcggagaa gagtcacacg aggacgcgga gttcgtcggc
     5641 gcactgtgcc gcaacttgaa cgtgcgccac gaggtgcggt ggatcagtct cgacggtagt
     5701 tccaacctcc aggaacgggc gcgtgacgag aggtacaggc tcgccgaagt ggtggccgcc
     5761 gggatgggcc ttggtgtcat agcgacgggt cacaccgccg acgacgtggc tgagaccgtg
     5821 cttatgaacc tggcgcgggg cacgggcctg cgcgggctcg ccggcatccc gcccgtgcgg
     5881 ggtaaggtgc agcgtcccct tatcgggtgc acgcgcagtg agatcctgga ttacctgaga
     5941 gagatagacc agccctatcg tacggaccct acgaacctga ccggcaagta cgcccgcaac
     6001 cgcgtccgtc tcgaagtcct ccccgtactg gaggagctct atccggcggc gacaggcaac
     6061 atcgcgcgcg ccgcctcgct catccgcgag gacctcgaat tgctcgaaga gcttgcggcc
     6121 ggaaccttac agttgagggg agaggaggtg gtcttgcccc tttccgagct catgaacttg
     6181 cggcctgcgc tccggcgcca cgccgtgcgc ctggcatacg cgaccgtggt cccggataac
     6241 gcgccgctac cgtccaatct catcgaagcg gtgctcgagc ttctggaagg cggtgagggt
     6301 acacggacgc tggacctgcc gggtggggtg gtcgcctcgg gtcgccccgg agaggagctc
     6361 gcattctaca aggggcggcg gtcgatggtc tcggggcgag aggagatcaa ggccggggag
     6421 gcggtggtgt tcggggggtg gaggatcacg gcgcgggaga tctctggcta cgatccctcg
     6481 gatgcggcgc gccccgaggt tgcctacctc gatgccggaa atgggcctta ccaggtgcgg
     6541 atggcgcggg agggggatat catccggccg ttgggcctcg gtgggaccaa gaacgttttc
     6601 agggcgatga tggacaggaa ggtaccgagc gacctcaggc gcgggatgcc cgtggttgtt
     6661 gacgggcggg acgaggtcgc ctggatagtg ctcggagagc tcgacgagcg gtacaaggtg
     6721 gatgcgagga cggagaagat actcaggttg gaggtggcaa gaatctcgtg agtatgtcca
     6781 gtatgatgcc cgacgtgaaa gaggtcctca taacctctcg ggagatcgag gagaaggtgc
     6841 gtgagatcgg ggcgcgcatc accgaggact actggggcga gaatccgctc ctgatcggtg
     6901 tcttgcgcgg cgcggtcgtg gtgatgagcg acctcatgcg caacatagac ctgaagtgtg
     6961 agctggattt catggacata agctcctacg gcagtggcac ctcatcgagc ggcgtcgtcc
     7021 gcatcctcaa ggacctcgaa gaggacatca cagaccgtca tgtcctgatc gtagaggaca
     7081 ttatagacac gggtctgacg ctctcctacc tgaggcgttc cctgctcgcc cgcaagccgg
     7141 cctcgctcga gatctgcgcc ctcctttcca agccgtccag gcgtcgtacg gacctcgacg
     7201 tcagatacct tggcttcgaa gtgcccgacg agttcgtcgt cggatacggg ctcgactacg
     7261 ctggggccta ccgcaacctc cccgacatct gcatcctcaa gcccgaggtc ttcgcgaccg
     7321 gctgagagaa cgagcgtcct attcagtgcg caggtagtat aattcccatg tcctagacgc
     7381 acttcacgga aggtaagaag ctttgggcag aatcttgaga agcggcgggc tgctgtactt
     7441 cgtcatcctc atagtcttgg ccgtggtcct cgtaaacatg ctgagcaagg gcaaccccga
     7501 cgtggccgag ctcaattccc agcagtggaa tcaggcggta cagaacagga ctttcgacac
     7561 cagcctcgaa aagaccaacg agaactacct gacgatctac gacgaagatc agacggtaag
     7621 gggccaactg aataccgatt ctggaagcac gaaggaattc gagtactcct acacgcagat
     7681 gaccgacgtg gcgagccagc tcgacaaggc ggatgtgccc tattcggtgg acccgcagaa
     7741 cacgggcttc tggctcaccc tgctcggcac cctggcaccg atcctgctta tcgtgttgtt
     7801 cttcatcctg ttcatgagct ccatgcaggg cggcgggaac agggtcatga gcttcggcaa
     7861 gagccgggcc cgtaggatga ccaaagacca gcccaaggtc accttctccg acgtcgccgg
     7921 ggccgacgag gccgtacagg aactcaccga gatcaaggag ttcctcgagg cgccccagaa
     7981 gttccagaag ctcggcgccc gcatacccaa aggcgcgctc ctcgtcggtc ccccggggac
     8041 gggcaagacg ctcctcgccc gcgccgtcgc cggcgaggcc ggtgtgccct tcttctccat
     8101 ctccggctcg gacttcgtcg agatgttcgt cggcgtcggc gccagccgcg tgcgcgacct
     8161 attcgagcag gccaagcaga actcgccctg catcatcttc atggacgaga tcgacgccgt
     8221 aggtaggcag cgcggcgcgg gcctgggagg cggccacgac gagcgcgagc agaccctgaa
     8281 ccagctcctc gtcgagatgg acggcttcga ctcgaag
//
LOCUS       DATMVF010000258        19867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_539429, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000258 DATMVF010000000
VERSION     DATMVF010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19867
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539429"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VN178_13000"
     CDS             complement(<1..279)
                     /locus_tag="VN178_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWS81931.1"
                     /translation="MSNRAGGQAGEFNPTSREFGLPLPEQIAEILATEIIERELTPGS
                     RLPEPALAERFGTSRAPIREALYLLDQAGLVERTPRRGTVVKAYSRREI"
     gene            complement(572..1438)
                     /locus_tag="VN178_13005"
     CDS             complement(572..1438)
                     /locus_tag="VN178_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS81932.1"
                     /translation="MITRFDHAVIAVADLRRAIEAYGALGFDVFPGGRHEHRGTHNAL
                     IRFGGADYVELLGVYDSERAIESGLNGRTLAEFVRHREGGLVGHCYATDDIEAEADRT
                     REAGLEMVGPFEMKRERPDGRALTWRLLVPVDIPWRRRWPFFIEWDDPDEVRLAVEGV
                     GDHPNGARSVSSVAVAVRDLEEAVRLYSILFDLEPSRRDEITDLAARRASFDVRGFAI
                     DLLSPAGTGPVQEMLERDGEGPFEVKIEVEDLTKARLALSGFESMENAALRPAADASL
                     GVHLVFTGQTGS"
     gene            complement(1435..2448)
                     /locus_tag="VN178_13010"
     CDS             complement(1435..2448)
                     /locus_tag="VN178_13010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWS81933.1"
                     /translation="MGERNAKFGLTLPNRGVITGATTMDEMLTLAQMADKEPIWDSVW
                     VGDSILAKPRLDAIALLGALAVKTERVKLGPACFASAPLRNALLLAYQWASLDYMSGG
                     RTIFVACQGQAAAGRGGNFANEFAAFGIDPSSRMRRMEEAVEIMRLLTSEEGAGYEGE
                     YNRFENVTVLPRPVQQPVPIWLVANPDPAKKRNVETSLRRVVRLADGWQTTWNTPESF
                     AQHLSTIKGYAGEEGRDLGDDFEACLYYNINVGEDREAALDEAKRFLDAYYYTDYDRE
                     RLKRWVAYGSPEECVEQIRAFIEAGATTVTLRMAAYDQKGQFERVTREVLPALQSAFS
                     REP"
     gene            complement(2486..3769)
                     /locus_tag="VN178_13015"
     CDS             complement(2486..3769)
                     /locus_tag="VN178_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWS81934.1"
                     /translation="MSSEESATTIAREDTPRAIGERLRIIGPGLVLAATTVGIGDFIS
                     NAVAGERYGMTFAWAIVLAVVLKYFLTEGLGRWHLASGQTIIYGWNSLGRGMTVFVAV
                     YLVIWAFFFGAAGPSVVGLATNAMFPILSVPAWAVIHSLLAFLFVWVGRYRLFENVMK
                     VLIACKVVVVVVIAALLRPDLGEIASGLVPKVPAGSLLYAVGIVGGLGGTLALASYGY
                     WVRDKGWRSPSWVPIMRLDAGLGYVVTAIFGMAVMVIGAELLFETGRSIGDEEGLVNL
                     AAPLGERFGSVVRWVFLAGLWAISFASVIGVWNGMSYLFADIVRTFRGIPEEEAGPYI
                     SERSPAYRSFLILLTFPTIPLIVLGQPVALVLLWTAMGAVFLPFLSITLMWLLNSSRV
                     ERGYRNRLVSASNIVLGISVVVFLVLGIQTIVGLL"
     gene            4298..4930
                     /locus_tag="VN178_13020"
     CDS             4298..4930
                     /locus_tag="VN178_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81935.1"
                     /translation="MKFLSGLGAFSNAIALLMLVAFPAVALVESRVGGTKGNDWGSRS
                     YPNWNLPHDCGGHEQRGALFDATGAGDARIWGNDGCDLIPSGEGNDTVYGGKEMDALF
                     GGRGNDKLKGHKGHDHLFGGSGSGISSGNDLLDTRDGENERGEIEEVHGADGFDRCFL
                     DPDPDGVKFSGCEYLNGRKSPYPTDKYVNTSDEGNQTDRRAEVNQFLRGN"
     gene            5260..6192
                     /locus_tag="VN178_13025"
     CDS             5260..6192
                     /locus_tag="VN178_13025"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWS81936.1"
                     /translation="MTQDLLKDGKSGRFFRGNLHCHSNRSDGLVEPAEVVAAYREAGY
                     DFLCLSDHFEQEHGWRITDTRPFRNEDFATILGAELSTAPWDERDCYWVTAAGLPPDF
                     APPPPDDQAEAIRRAANLGAFVVMLHPGLNNLPLEAVDGLPGLEAVHAVEIYNHNCAM
                     AALPDRAYGAYMLDGLLEGGRMLLVNAGDDAHFGHPLDRFGGWVEVHCDRLDPNALLD
                     SLKAGRYYSTQGPQLRELLVDGDRLRVGTSDVYAISLTGGGDRWQSGQERHGEPGAPI
                     TEAEFDVAPFRGSYCRITVVDVAGKRAWGNPIWP"
     gene            complement(6431..6937)
                     /locus_tag="VN178_13030"
     CDS             complement(6431..6937)
                     /locus_tag="VN178_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWS81937.1"
                     /translation="MPPNSGREEAVGRFIERFALTLSEAGIPRMPARVFVALLASDDG
                     RCTAGELAETLKVSPAAVSRAVGYLQQVGMVAREREPGERRDHYRVYDAAWYEAVVRW
                     DEMLARWQEDLREGIEAVGADTPAGKRLEETRRFYEFLHDEMPMLMERWREVRSGQHS
                     SQGEDSEG"
     gene            7191..7436
                     /locus_tag="VN178_13035"
     CDS             7191..7436
                     /locus_tag="VN178_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81938.1"
                     /translation="MRKTAVYGTVMVFLTTTLLHAVSHFGQEVLSLEVWQWAYVIGVI
                     YLAQIAAGVTLKPHSGFWAISVPAILALGTRRRNGAG"
     gene            7498..8082
                     /locus_tag="VN178_13040"
     CDS             7498..8082
                     /locus_tag="VN178_13040"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWS81939.1"
                     /translation="MGKTYDCITDELARWFEAQPVFFVATATSDPKTRVNLSPRGLDT
                     FRVLDNRRVAWLDLTGSGVETIAHLRADGRITFMFCAFNGPPRIVRLHGHGRVHEPGD
                     GAYEMLRPRFPDLPGGRAIIDVEISRVASSCGFGVPRMEFLGPREQLVTFAERKASGE
                     LAEYRARKNVRSIDGLPGLESPNTIQTGPEDGAA"
     gene            8267..8653
                     /locus_tag="VN178_13045"
     CDS             8267..8653
                     /locus_tag="VN178_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81940.1"
                     /translation="MTQKASEYGTIGSRVEGPRIAVSVATTPPKVKAYNVADEPVNWR
                     DFTWALAQAYRTPRPFELPRCVLRLVAPYLAFMMTSTLRASNARAKRELDWTPSLPSY
                     RDGIRHMTAFHGVRSTQSDSRKVRMP"
     gene            8876..10135
                     /locus_tag="VN178_13050"
     CDS             8876..10135
                     /locus_tag="VN178_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWS81941.1"
                     /translation="MSIENTEILVSGAGVAGSVLAYWLERYGFNPTVVELAPTIRKAG
                     GHAVDLWGSAVDVVERMGILPEVEAARTRNDASSLIRPGKPPIDMDVGELAVEFSDRV
                     VEIPRDELTRLLYRPTSNDVEYIFGDSIAALEEDADGVRVAFQRSAPRRFDLVVGADG
                     LHSRVRRLAFGTEERFRRYLGGYIAVYTVPNFLGLDGRILTYSAVDRTVVVYPVWKSG
                     DARALFMFRRAEELDYDHHDVQQQKRLLRGAYAAGGWEVPRLLEYLEGTEAFYFDSIS
                     QIRMDAFSRGRVALVGDAGYAPGAGVGGGTSLAVVAAYVLGRELAAAGGDHTAGFRNY
                     EDAIRDTVARSRAIGPALLKTLIPTSRAQIWLGMQVARLLPRLPKRLKRSISLFPREA
                     VQGLHTIASLPLEDDRTKATMKARQES"
     gene            10236..10640
                     /locus_tag="VN178_13055"
     CDS             10236..10640
                     /locus_tag="VN178_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWS81942.1"
                     /translation="MRASIVGAGIGGLTTAIALRRAGIETTVFERAGKLREVGAGLLL
                     AANAVRALDGLGLSEKVRQIGTPASAARILSWHGETLEAGRLEARAVDPYRLEDPELE
                     SLVRVLRGPGAAGGRLPSEAESRRLPHGGCQA"
     gene            complement(11447..11698)
                     /locus_tag="VN178_13060"
     CDS             complement(11447..11698)
                     /locus_tag="VN178_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81943.1"
                     /translation="MAICLIPQRISAAARDAVSLERPVARRSSSLFVWLARTKSFPRQ
                     FARLMANIPATWAVVGLAGAMRLSRRSGPLVAASRRGPT"
     gene            11867..12700
                     /locus_tag="VN178_13065"
     CDS             11867..12700
                     /locus_tag="VN178_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS81944.1"
                     /translation="MPEVELSAGTIEYEDTGGEGPVFVLLHGVGMDGSLWRHVVAELR
                     PDFRCVVPTLPLGGHRRPMRSDADLSLRGMVRLVAEVLEKLDLRDVTLVGNDWGGAQL
                     LVSEGVDGRVGRLVLAPCEAFDNYPPGVPGRALAARTPGGLLLAFQALRLRPLRRLPM
                     TWGWMSKRPVSAEVMDRWFRPLQTSKEIRRDLRKYALSIPPKEVLLGWHEDLRSFHRP
                     ALVVWASEDRVMPPEHGRRLVGLFPHGRLVEVPDSYTLIPEDRPGELARAVRDFVRDT
                     P"
     gene            12827..13774
                     /locus_tag="VN178_13070"
     CDS             12827..13774
                     /locus_tag="VN178_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HWS81945.1"
                     /translation="MNHGDSGGCPQDAARVFEEYRGLLVSVAYRVLGSVTDAEDVVQE
                     AWLRWSNVNHSEVVDPRAFLVRVSTRLAIDQLRRVKARRESYVGPWLPEPILTGRDLS
                     EDAAMADSVSMAMMVVLETLSPLERAAFVLREAFGMPYAEIADVMGRREEAVRQLAKR
                     ARDHVRERRPRFDADSDEQRRVTERFLEATSNGDLEALMGVLSPEVVLVADGGGKALS
                     PRRPVLGAEKVARFLVAIVGEEQIVRFLRSVGDGSGPSGEVRVHLAPVNGETGVVVTA
                     GAEPISALVLDVANDLVRTIRVVANPEKLAGLRGIETTR"
     gene            13771..14373
                     /locus_tag="VN178_13075"
     CDS             13771..14373
                     /locus_tag="VN178_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81946.1"
                     /translation="MRVISWTSVLNLFVVFSLFACSPEEVHRPDRDENPEESIRVGGG
                     PFPGGEARTIARTGGRVSVERAVLSFAGEADLIHARVEYEGSWVWPRCSLLEGPDVET
                     VRRTIETREPPTAQGYTKPLWEESLWREHESLWADRSTIEKSLQDGGAVLWTFHEDPK
                     PEGEVADPRRTPFFVRCAGGDLPDVVHDVAHVLGTPGTAS"
     gene            14376..14819
                     /locus_tag="VN178_13080"
     CDS             14376..14819
                     /locus_tag="VN178_13080"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HWS81947.1"
                     /translation="MASSRGVSARSHERRETAKAPRRATLVFDGRCGVCTRFVRAIER
                     LDRKQRITALPCQMPGVPASVGLSAEECRKAAWVVTPGGYRYRGAGAVNLALAVALGT
                     KLPYAFYRLPLIKCAQDRAYDWIAANRHRLPGDVPHCSQHPGECA"
     gene            14839..15138
                     /locus_tag="VN178_13085"
     CDS             14839..15138
                     /locus_tag="VN178_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81948.1"
                     /translation="MTSRSKGVSWPWWVQPLVVGGSLAAFIAYSTWAVFQTGGYYADP
                     YFSPFYTPCLAARCGEAATFPLLGGWWTLSPALLVAGHLLLLPQGVLPGLLSGSP"
     gene            15206..15577
                     /locus_tag="VN178_13090"
     CDS             15206..15577
                     /locus_tag="VN178_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81949.1"
                     /translation="MVNNLHRYFWYAGVLIAVVLTYDTVVAFRFSDGWGVGLGSLIFL
                     LNAAAFWLYSLSCHSCRHAVGGRLKHFKRHPLRYRFYNLVSKLNHHHPAFAWISLPAM
                     VFTDLYVRLVASGVVTDLRFF"
     gene            15956..16825
                     /locus_tag="VN178_13095"
     CDS             15956..16825
                     /locus_tag="VN178_13095"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWS81950.1"
                     /translation="MRVLTWNLWWRFGAWERRREVIIAVLDDLRPDVCGLQEVWSVPG
                     ENLAASLARELGMHHAYAPSPAPGKWQRRIGDHGVGVGNAVLSRWPISESDVRRLPIG
                     DEPDEGRTVLYARIDAPHGKVPIFVTHLNSGLGQSALRKEQLGSVAGFMRGKGQDGHP
                     PILTGDFNAPPDADEVRCLVGKAPPPARGFVLADAWDYARPLEPGWTWNRRNPHVAAT
                     FEPDARIDYVFVGRPDGEGRGQVLDVGLVGTEPVGGVWASDHWGVLAVLRGGPKHEDQ
                     GSGPSKEPGGCPG"
     gene            17122..17427
                     /locus_tag="VN178_13100"
     CDS             17122..17427
                     /locus_tag="VN178_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWS81951.1"
                     /translation="MRPNNRIPLETLRRSLGRVAQDVERGHSVVVERRGEEAFALVPM
                     RIYRLLEEERRALVESTRQAREAFSHLSEGEVEELVEGEIEATRSRAAPSVDGAADR"
     gene            17431..17865
                     /locus_tag="VN178_13105"
     CDS             17431..17865
                     /locus_tag="VN178_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWS81952.1"
                     /translation="MRVVVDANVSGVLSAGGNPAKILDHAFGEGPYDFELCAPPQMFS
                     KVEEVLARPRIASRLQWGPAEIGVYSRRLRLAVTEVSTGDPEKIPSYTEDPEDDPYIQ
                     AAVLGGAAYVVSGDDDVLRMEDPPVPVLSPAQFVRLWKARLL"
     gene            complement(18518..18614)
                     /locus_tag="VN178_13110"
     tRNA            complement(18518..18614)
                     /locus_tag="VN178_13110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18531..18533),aa:Ser,seq:cga)
     gene            19070..>19867
                     /locus_tag="VN178_13115"
     CDS             19070..>19867
                     /locus_tag="VN178_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81953.1"
                     /translation="MDRIRSLSTEPMREALLGLLLAAGVSLLLVVGFYILLLIRWVIG
                     MGDAPGMGGLVSFVAAHGGALSVRVPPGPALLDIGGSLKIDPPITSIALLPFILLLLG
                     SWMISRRLRAFVLFPLVATVCYSVILAVVALLSRTTTSGEGSEVTVSAAPLSAAFHGL
                     LIAGLGTILGLVAARGPFLTERTRQVMRGALVAVGASTVLALVLAVLVLLVTLVTSGP
                     ENPLGNLPQDAGPQPSDNAQPNGAKPDGGGVGNSIRTLLTAIGGMFAL"
BASE COUNT         3568 a         6271 c         6479 g         3549 t
ORIGIN      
        1 tatttcacgc ctggagtatg ctttcaccac agttccgcgg cgcggcgtgc gttctaccaa
       61 tcccgcctgg tccagcagat agagggcctc ccggatcgga gccctgctgg taccgaatct
      121 ctcggccaac gccggttccg gcagtctgct ccctggggtt agttcccgtt cgatgatctc
      181 ggttgcgagt atctccgcga tctgctccgg cagcggtagt ccaaactccc ggctcgtcgg
      241 gttgaattct ccggcctgac ctcccgcacg gttcgacaaa gagatccctt ctccactcct
      301 ccagttccac ggcgagcttg gcctcaccac ttttgtgatt cgacgtagtt tatctacctg
      361 cccgacgtca cttggtccta agcgaccccc cacactttga gactacgcag gagcgcactg
      421 aatccttagt ttacaatctc ttggactcta ctcacagagt atggtcgggg ctcgaggtca
      481 gatctcttga tgctagcggt tcgaagcaag agagatctac atctagcatg cagcacttca
      541 gctagagtcg gattctcgtg ggcccggctt cttaggatcc cgtctgcccg gtgaacacca
      601 ggtggacgcc gaggctggcg tccgccgccg gacgcaacgc tgcgttctcc atggactcaa
      661 agccggagag ggccaggcga gccttcgtaa gatcctcgac ctcgatcttt acctcgaagg
      721 gtccttcgcc gtccctttcg agcatctctt gcacgggtcc cgtgccagca ggcgaaagca
      781 ggtcgatagc gaagccccgg acatcgaagc tggcgcgcct ggcggcgagg tcggtgatct
      841 cgtctcttcg agagggctcc aggtcgaaaa ggatggaata cagccttact gcctcctcga
      901 ggtctcttac ggcgacggcg acactggaga cagatctcgc gccgttgggg tgatccccta
      961 ctccctccac ggcaagtcgc acctcgtccg ggtcgtccca ctctataaag aacggccacc
     1021 ggcgcctcca cgggatgtct acgggcacca gcagacgcca ggtgagtgcc cgcccgtcgg
     1081 gcctttcccg cttcatctcg aagggtccga ccatctcgag tcctgcctcc ctcgtgcggt
     1141 cggcctctgc ctctatatcg tcggtggcgt agcagtgccc gacgagtcct ccttccctgt
     1201 ggcgcacgaa ctcggcgagg gtgcggccgt tgaggccact ctcgatggct ctttcagaat
     1261 cgtagacccc gagtagctcg acgtagtcgg cgccaccgaa gcggatgagg gcgttgtggg
     1321 tgccgcggtg ctcgtgtcgt ccgccaggaa agacgtcgaa gccgagagcg ccataggcct
     1381 cgatggcacg gcgcaggtcg gcgacggcga tcacggcgtg gtcgaagcgg gtgatcatgg
     1441 ctctcggctg aaggcggact gcagagcggg cagcacctcg cgggtgaccc gttcgaactg
     1501 gcccttctgg tcgtaggcgg ccatccgaag ggttaccgtc gtagctccgg cctcgatgaa
     1561 cgcccggatc tgctcgacgc actcctcggg cgagccgtag gcgacccacc tcttgagacg
     1621 ctcccggtcg taatcggtgt agtagtaggc gtcgagaaac ctcttggcct catcgagcgc
     1681 ggcctcccta tcctcgccta cgtttatgtt gtagtagagg caggcctcga aatcgtcccc
     1741 gaggtcgcga ccctcctccc cagcgtagcc cttgatggtg gagaggtgtt gggcgaagga
     1801 ttccggggtg ttccaggtgg tctgccagcc gtcggcgagg cggacgacac gcctcagcga
     1861 tgtctcgacg ttccgcttct tggccgggtc cgggttggct acgagccaga tggggaccgg
     1921 ctgctgcacc ggcctcggca gcactgtcac gttctcgaat cggttgtact cgccttcgta
     1981 gccggcccct tcctcggaag tgagtagccg catgatctcc acggcctcct ccatgcgcct
     2041 catcctcgag gaggggtcga tcccgaaggc ggcgaactcg tttgcgaagt tgccgccgcg
     2101 tccggcggcc gcctgtccct ggcaggcgac gaagatcgta cgtccgccgg acatgtaatc
     2161 caggctagcc cactggtagg cgagcagaag ggcgttcctc aagggagcgc tcgcgaagca
     2221 ggctggcccc agtttcaccc tttcggtctt gaccgccaga gctccgagca gcgctatggc
     2281 gtcgagccgg ggcttggcga gaatcgagtc gcccacccac accgagtccc agatcggctc
     2341 cttgtcagcc atctgggcca gcgttagcat ttcatccatg gtggtagcac ccgttatgac
     2401 cccgcggttc gggagtgtca gcccaaactt ggcgttgcgt tcgcccaccg tttccacctc
     2461 ctcgaatgtt cgcgagcgac ccggttcaca gaagccctac gatggtctgg atccccaaga
     2521 ccaaaaagac gactacgctg ataccaagga ctatgttgga ggcggagacc agcctgttcc
     2581 gataaccgcg ttccacgcgg gaggagttga gcagccacat caatgtgata gagaggaagg
     2641 gcaaaaacac ggcacccatc gccgtccaga gtagcaccag ggccaccggc tggccgagca
     2701 cgatcagagg tatcgtgggg aaggtgagaa ggatcaggaa ggagcgatag gccggactcc
     2761 tttccgagat gtagggtccg gcctcttctt ccgggatgcc ccggaacgtc ctcacgatat
     2821 cggcgaagag gtaggacatg ccgttccaca ccccgatcac cgaggcgaat gagatcgccc
     2881 acaaaccggc caggaagacc cagcgcacga cgctcccgaa gcgttcgccc agcggggcgg
     2941 ccaggttgac caggccttcc tcgtcaccta tgctccttcc ggtttcgaag agcagctcgg
     3001 ccccgatcac catcaccgcc atcccgaaga tggccgtgac cacgtatccg agtcccgcat
     3061 ccaggcgcat gatcggcacc caggacgggc ttcgccagcc cttgtcgcgc acccagtagc
     3121 cgtaggacgc cagggccagc gtaccaccca gtcctccaac gatacccacg gcgtagagga
     3181 gtgaaccggc cgggaccttc ggcaccagac ccgaggctat ctccccaagg tcaggccgca
     3241 acagcgcggc gatgacgact acgacgacga ccttgcaggc gatcagtacc ttcataacgt
     3301 tctcgaagag ccggtatcga cccacccaga cgaacaagaa cgcgagaagg gagtggatca
     3361 cagcccaggc cgggaccgac agaatgggga acatcgcgtt ggtggccaac cccacgacag
     3421 acgggccggc ggcgccgaaa aagaacgccc agatgaccag gtagacggca acgaaaacag
     3481 tcatccctcg tccgagagag ttccacccgt agatgatcgt ctggccagac gccaggtgcc
     3541 accgccccag cccctctgtc aagaaatact tgagcaccac ggcgaggacg atcgcccagg
     3601 cgaaggtcat cccgtagcgc tcgcccgcca ccgcgttgga gatgaagtcg ccgatgccca
     3661 ccgtggtagc agcgagcacc agccccggcc cgatgattct gagacgctcc cctatcgctc
     3721 ggggggtgtc ttctcgggcg atcgtggtgg ctgactcctc gctgctcaag cgtttgctcc
     3781 attcgaggac gacagatcgt ggacagcatg aatgcgcgcg caagaggcaa gtatcatcga
     3841 ccaggttccc cagacgctgg tccttgtcgc cttggactgc actcgcggtt attatccgac
     3901 aaagcgagtc ggagtatagc gattgtcgcc gagcggtcaa tgattcgtgg tatctgaaca
     3961 acgtgcgtaa atccgactgg aagtggaccc aacaggtgcg gccgaacgcg ctaccggaag
     4021 tctgacgagg gacgtccggg ggcagaacag ctgtcttggt cggcctcact ttcgacgggg
     4081 aacaaatccg taccacacgt aggctagacg aggctcgtac acgtgaagcg acgttacgcc
     4141 cgtgcctcga acaccttggg agattgccaa caagtcatga gtggggcaca atagatcagg
     4201 cgggtgggta ctggagtggg atctcaacag ccacggtcac ctgtagcacc aacacagcca
     4261 agggactcat agacggctaa ggagggttca aactaccatg aaattcttga gcggcctggg
     4321 tgccttctcg aatgcgattg ctctattgat gctagtcgcg tttccggcgg tggccctcgt
     4381 agagagcagg gttgggggca cgaagggaaa cgattggggc tctcgctctt atcctaactg
     4441 gaatctcccc cacgactgcg gcggacacga acagcgcggc gctttgttcg acgctacggg
     4501 ggctggggac gctcgtatct ggggtaatga cggctgcgac ctcatccctt caggcgaggg
     4561 gaatgacacc gtctacggcg gcaaggaaat ggacgcgcta ttcggcggcc gaggtaacga
     4621 caagctcaag ggccacaagg gccacgatca cctcttcggc ggctcggggt cgggtatctc
     4681 ctcgggcaat gacctcctcg acacccgcga cggcgaaaac gagcggggcg aaatagaaga
     4741 ggtgcacggg gccgacgggt ttgataggtg tttcctcgac cctgaccccg atggggtgaa
     4801 attctcaggc tgcgagtacc tgaatggccg caagagtcct tacccgacgg acaagtacgt
     4861 caacacgtcc gacgaaggca atcagaccga ccgcagggcc gaggtgaatc agttcctccg
     4921 gggaaactag gcagctttcg tgtgccggta cccgcgtgat gccgtgctcg gagatgcctc
     4981 aggcgaagct gtcgccgaaa gcgccgccga actcgccgag gagatcggtg cgctagaagg
     5041 ttgggtcaac aacgccgccg tctttaggga cgcccaggtc cactctgacg gagcacgcga
     5101 cgtgctcgac gccatcacta gttcgtttgg ctcgaagtcg cacaggcggg tcacgatata
     5161 cgtgatggcg tcggcaatgt cttcagcctg caggcattga acgtttgcga agaagtggaa
     5221 ccctggtctc gcccatccat caacgagagg gaaatcgcca tgacgcagga cctgctaaag
     5281 gacggaaaga gtggccgctt cttccgcggc aacctccact gccactccaa ccgctccgac
     5341 ggtctagtcg agcccgcgga ggtcgtggcc gcctaccgcg aggcgggata cgacttcctc
     5401 tgcctctccg accacttcga gcaggagcac ggctggcgga taaccgacac gcgacccttt
     5461 cggaacgagg acttcgccac gatcttgggc gccgagctct ccaccgcccc gtgggatgag
     5521 cgtgactgct attgggtcac ggccgccggc ctgcccccgg acttcgcgcc tccgccgccg
     5581 gacgatcaag ccgaggcgat ccggcgggca gcgaacctgg gggcgttcgt cgtcatgctg
     5641 cacccgggac tcaacaatct cccgctggag gcggttgacg gtctgccggg actagaggcc
     5701 gtgcacgcgg tcgaaatcta caaccacaac tgcgcgatgg ccgccctccc cgaccgggct
     5761 tacggcgcct acatgctcga tggacttctg gaaggtggcc gcatgttgct ggtgaacgcg
     5821 ggagacgacg cccacttcgg ccacccgttg gatcgcttcg gcggatgggt ggaggtccac
     5881 tgtgaccggc tcgatccgaa tgccctgctg gattcactga aggccgggcg ttactactcg
     5941 acccagggac cgcagctgcg cgagctgctc gtcgacggcg atcggctacg ggtgggaacg
     6001 agcgatgtat acgcgatctc gctgaccgga ggtggagacc ggtggcagag cgggcaggag
     6061 cgccacggtg agcccggcgc accgatcacg gaggcggagt tcgatgtcgc gcctttccgc
     6121 ggctcctact gccgcatcac cgtggtggac gtggccggga agcgggcctg gggcaacccg
     6181 atctggccgt gaggcaggac gatgctcagt tgcgcaagcg tcgtggtaat cgaggaacac
     6241 cgtcttcgga tcagaccgct ctgccgatgg ttagagatac cggccggtaa gctgagagcg
     6301 cagcatcctg cgcggagaat caggtgatgc gggtaggcag acgcgacctg gcccacctcg
     6361 tctctcgagc cacctgggtc ccggagtcct gaccagcgtg ctcgcgcgga tcgtgacgac
     6421 gtcaggatgc tcatccttcg gagtcttcac cctgagatga gtgttgcccc gaccgcacct
     6481 cgcgccacct ctccatgagc atcggcatct cgtcgtggag gaactcgtag aaacggcgcg
     6541 tctcttcaag ccgcttccct gccggggtgt ccgcgccgac ggcctcgatc ccctcccgca
     6601 aatcctcctg ccagcgtgcc agcatctcgt cccaccggac gaccgcctcg taccacgctg
     6661 cgtcatagac ccggtagtgg tcacgacgct ccccaggctc ccgctcgcgc gccaccatcc
     6721 cgacctgctg caggtagccc accgcgcggg ataccgcagc cgggctgacc ttgagcgtct
     6781 ccgcaagctc gcccgctgtg cacctgccat catctgaggc gaggagcgcg acgaacaccc
     6841 tggccggcat acgagggatc cccgcctcgg agagcgtgag tgcgaacctc tcgatgaacc
     6901 gccccaccgc ctcctcccga ccgctgttcg gcggcaccta cctttccacc ctttcacttc
     6961 gccgcttcat cttccccctc ggtactcgac agctccacat agcttcctca tcgcacagat
     7021 tgaaagtatt gacaaatgtg tcaaaacagt atagcatcca aatgaagaac aagaaacccg
     7081 ccgatgaaag tggtgtgaga tcaccgtcga cgtcacggta gtcgttactg ttccgtccca
     7141 atcacgacga tcctcaggat tttgaagcag accaaccagg gaggaagagg atgcgcaaga
     7201 cggcggttta tggaacggtg atggttttcc tgacgacgac cctgctgcac gcggtctccc
     7261 acttcggcca ggaggttctg tccctggagg tgtggcagtg ggcgtacgtg atcggggtca
     7321 tatacctcgc ccagatcgcg gcgggggtca ccctcaagcc gcactcgggg ttctgggcca
     7381 tctctgtccc cgcgattttg gccctcggta ccaggcgccg aaatggtgcg ggctgatgcc
     7441 gatgatcttg gtggcgttgc ggatccaacc tccggagacg tgggaaggag caggacgatg
     7501 ggaaaaacat acgactgcat aaccgatgag ctggcccgtt ggtttgaggc gcagccggtg
     7561 ttcttcgtgg cgaccgccac ctcggacccc aagacgcgcg tcaatctatc gccgcggggg
     7621 ctggacacgt tccgagtcct ggataaccgg cgggtcgcgt ggttggacct caccggcagc
     7681 ggcgtcgaga ccattgcaca tctcagggcc gacggacgca taaccttcat gttctgcgcc
     7741 tttaacggtc cgccccgtat cgtccgtctg cacgggcacg gccgcgtcca cgagcccggc
     7801 gacggcgcat acgaaatgct gcgtccccgc ttccccgacc tgcccggcgg gcgggcgatc
     7861 atcgacgtgg agataagccg ggtagcgagc tcttgcgggt tcggggtgcc gcgcatggag
     7921 ttcttgggac cgcgcgagca gctggtcact ttcgccgaac gaaaggcctc cggagagtta
     7981 gccgagtacc gggcacgtaa gaacgtgcgg agcatagacg gactccccgg cctggagtcc
     8041 ccgaatacga tccagaccgg gccggaagat ggagcggcgt aacgccaaca aggtagtccg
     8101 agagtagccg tgttcgatcg tgactaccgc agcaggagaa gcaggcgaca gacaagccaa
     8161 ggaggagagg cgtgagactc accatcagtc ctgttcgttg tacaccgcgc gaggatgagc
     8221 gtgcgaagaa taggttcggg atcttcacca caagaaagga gcactgatga cccagaaagc
     8281 atcagagtac ggaacaatcg gctcccgagt ggaaggaccc cgcatcgcgg tctcggtagc
     8341 tactacaccg cccaaagtca aagcctacaa cgtggcggac gagccggtga actggcggga
     8401 cttcacatgg gcattggccc aggcgtatcg gactccgcgg cccttcgagc ttccgcggtg
     8461 tgtgcttcgc cttgtcgcgc cctacctcgc cttcatgatg acgagcacgc tgcgggcctc
     8521 gaacgccagg gccaagcgag agctcgactg gacgccgtct ttgcccagct accgtgacgg
     8581 catccggcac atgacggcct tccatggcgt gaggtcgaca cagtcggact cgagaaaggt
     8641 gaggatgcca tgaggaacaa cgaacaagca cagaagcatg ccgacgaacg tcccgcttcg
     8701 gaggcgggcg tcgccgcgat cgaggaggca gatcgttgga cgccaggatc ccggtccagt
     8761 cgaccccgca acatggcgat agaaatcgct cgtctcaatt tacatacgtg actgtatgag
     8821 attggcccgg acaaagttgg agagtgagat ggtagagcag aatcaggaac cacagatgag
     8881 catagaaaac accgagatcc tcgtctccgg ggccggggtc gccgggtcgg tcctggcgta
     8941 ctggctcgag cgatacggct tcaatcccac ggtggtcgag ctggcaccga cgatccgcaa
     9001 ggcgggcggc cacgctgtcg acctgtgggg gtccgcggtc gacgtcgtcg agcggatggg
     9061 gatcctgccc gaggtcgagg ccgcgcgcac ccgcaacgac gcgtcctcgc tgatacgccc
     9121 gggaaagcca ccgatcgaca tggacgtcgg cgagctggcg gtggagttct ccgaccgggt
     9181 cgtggagatt ccccgtgacg aactgacccg gctactgtac cggccaacca gcaatgacgt
     9241 cgagtacata ttcggcgact cgatcgcggc actggaggag gacgccgacg gtgtgcgggt
     9301 ggccttccag cgctcggctc cccgccgctt cgacctcgtc gtcggcgccg acgggctgca
     9361 ctccagggtg cggcggctgg ccttcgggac ggaggagcgg ttccggcgct acctcggcgg
     9421 ctacatcgcc gtctacacgg tccccaactt cctggggctg gatggccgga ttctgaccta
     9481 cagcgccgtg gacaggacgg tggtcgtgta ccccgtctgg aagagcggcg acgccagggc
     9541 gttgttcatg ttccgccgtg ccgaggagct cgactacgac caccacgacg tccaacagca
     9601 gaagcggctg ctgcgcgggg cgtacgcggc cggcgggtgg gaggtgcctc gcctgctgga
     9661 gtacctggag ggcaccgaag ccttctactt cgactccatc agtcagatcc gcatggatgc
     9721 cttctcgcgt gggcgggtgg cgctggtggg cgacgccggc tacgcccccg gggcgggggt
     9781 cggcggaggg acgagcctgg ccgtggtagc ggcctacgtg ctcggccgcg agctggcggc
     9841 ggccggcggc gaccacaccg cggggttccg caactacgag gacgcgatcc gggataccgt
     9901 tgctcgcagc cgggccatcg gcccggcgtt gctgaagacc ctgatcccca cctcgcgtgc
     9961 ccagatctgg ctggggatgc aggtggcgcg gctgctgccc cggctgccaa agcggctcaa
    10021 gcgatcaatc tccctattcc cgcgtgaagc tgtccagggg ctgcacacca tcgcctcgct
    10081 cccgctggag gacgaccgga ctaaggcgac gatgaaggca aggcaagagt cctgaaagag
    10141 aaagcacgga cgatcctggc ccgaaccagg ggtaggagcg gcgccggcct cgacccgccg
    10201 aacacgggca aacgcatgag aagagcgcga aagggatgag ggcgtcgatc gtcggtgccg
    10261 gcatcggcgg cctcacgacg gcgatcgcgt tgcgaagggc cggcatcgag accaccgtct
    10321 tcgagcgcgc cggcaagctc cgggaggtcg gcgccgggtt gctcctggca gcaaacgccg
    10381 tcagggcgct cgacgggctg ggtctctccg aaaaggttcg ccagatcggc accccggcct
    10441 cggccgcccg catcctctcc tggcatgggg agacgctaga agcaggtcgc cttgaagcac
    10501 gcgcggtcga cccctaccgc cttgaagacc ctgaactgga gtccctggta cgagttctcc
    10561 gcgggcctgg agctgccggc ggacgccttc catccgaagc cgaaagtaga cgcttgcctc
    10621 atggtggctg ccaagcgtga cccgccgctc gtggccgccg agcaccggca cttcttccgc
    10681 gcgttcgtgc gccgggcctt cgacggacat ggaaactgcg tgagtgaaac cctcaggccg
    10741 atcttcagca ggacccaact ccgcaggctc gcaagagaca acggattgtc tctgctttgg
    10801 ttcccttcca tgctcacggt gcgccagtgg gcgagtcttt tcgacgccat gatgctgctg
    10861 gccccgcgga atcgttggcc ttcgttgcga cggcccgcca agagagaaag ctggaattgc
    10921 tgctgagagg cttcttgagc ggatggtagg gacgacagtt cgtacctgtg ccgcccagcc
    10981 ggaaagcctg acgctggcgg ccgtcatcac gctgtggaac ttcccggcgg ccacgatcgc
    11041 gcctctagaa ctcaaaacgc aataaaccaa gttgagctgt ctaaggagag ggcttcttga
    11101 taaagcttgt tgcgtctcac gaccgcgaag gctaactttg cgctgagtgc acgtccggca
    11161 agagcgaggg ctggaggaca gcagcgccgg aggaggtctc gttcgtccgc cacgacgtca
    11221 cgctggcgcc cctgccccac ggtggggagg cggacctgat ctacgcccgt ctgctgctct
    11281 cccacctgcc agacccggag gaggtcgcat tggagtggct cgcgcagctc gcgccgggcg
    11341 gtctgctcct gctggacgag gtggagcgga tcgagacgga gcagccggtg ttccgcgagt
    11401 acctgggtat cctggcgcgg atgatggccc accacggcca ggagctctac gtgggccccc
    11461 gcctggaggc cgccacgaga ggaccggaac ggcggctcag ccgcatcgcc cccgcgagcc
    11521 cgacgaccgc ccacgtcgcc gggatgttcg ccatcaacct ggcgaactgg cgcgggaacg
    11581 acttcgtgcg ggccaaccac acgaacaggg aactcgaccg cctggcgacg ggcctctcca
    11641 gactgaccgc gtcacgggcc gccgcagaga tccgctgggg gatgaggcag atcgccatag
    11701 aaagggcccg tcgacagaaa gtgagggagg agcgtgttcc agaggaaacg ggccgtcagg
    11761 gcccgtcgca tcggggagtt gctccgtccc ggtggccggg acgggggcgc gtcgtagatc
    11821 gcctcgaaga gctcgaagtt agtatgatgg acgaaaggag atcgccgtgc ccgaggtgga
    11881 gctgtcagcg gggacgatcg agtacgagga caccggcggg gaaggtccgg tcttcgtcct
    11941 gctgcacggg gtcggcatgg acggctcgct gtggcgccac gtcgtcgccg aactgcgccc
    12001 ggacttccgc tgcgtggtcc cgaccctgcc tctgggcggg caccggcgcc ccatgcgttc
    12061 cgatgccgac ctctcgctcc gcggtatggt ccggctggtc gcggaggtct tggagaagct
    12121 ggacctgcgt gacgtgaccc tcgtcggcaa cgattggggc ggggcacagc tgctcgtctc
    12181 cgaaggcgtc gacgggcgcg tggggaggct ggtgctcgcc ccgtgcgagg ccttcgacaa
    12241 ctatcccccc ggagtcccgg ggcgcgcgct ggcggcgcgg acacccggcg gacttctcct
    12301 ggcgtttcag gccctgcggc tgcggccctt gaggcgcttg ccgatgacat gggggtggat
    12361 gagcaagcgc ccggtctccg cggaggtcat ggaccggtgg ttccggccct tgcagaccag
    12421 caaggagatc cggcgcgatt tgcggaagta cgcgcttagc atcccgccca aggaggtgct
    12481 gctggggtgg cacgaggatc tgcgctcctt ccaccgaccc gcgctcgtcg tctgggcctc
    12541 cgaggaccgg gtcatgcccc cggagcacgg gcgccggctg gtcgggttgt tcccgcatgg
    12601 gaggctcgtc gaggttcccg acagctacac cctcatcccc gaggatcggc cgggcgagct
    12661 agcgcgcgcc gtccgggact tcgtccggga cacaccgtga acgcgttcac gcccaccctg
    12721 gccctgttac ggagagccgc gggggtgtgt gtcacgcgta gcgcttgcgg agcgtcttta
    12781 gggcacaggg tgcacggttg cgagcatcgt agacggggtg atgcggatga accacggtga
    12841 ttcgggcggg tgtccccaag acgccgcccg cgtttttgag gagtatcggg gtttgctggt
    12901 ctccgtcgcg taccgtgtcc tgggcagtgt gaccgacgcg gaggatgtcg tacaggaggc
    12961 gtggctgcgc tggtcgaacg tgaaccactc ggaggtcgta gatccacggg ccttcctcgt
    13021 gcgcgtctcc acccgtctcg ccatagatca gctgcggcgc gtaaaggccc gccgcgagtc
    13081 ctacgtcggc ccctggctgc ccgagcctat cctcaccggg cgggatctct ccgaggacgc
    13141 ggcgatggcc gattcggtct cgatggcgat gatggtggtg ctggagacgc tctctccgct
    13201 ggagcgggcc gctttcgttc tgagggaagc gttcgggatg ccctacgcgg agatcgccga
    13261 cgtcatgggg cgcagggaag aggcggtacg ccagctcgcc aagcgtgccc gtgaccacgt
    13321 acgcgagcgc aggccccgct tcgatgcgga cagcgacgag cagagaaggg tgaccgagcg
    13381 cttcctcgaa gcgacctcga acggcgacct cgaggccctc atgggggtgc tctcgcccga
    13441 ggtggtgctg gtcgccgacg gcggcggcaa ggcgctctcg ccccgtcgcc cggtccttgg
    13501 ggcggagaag gtggcgcggt tcctggtcgc catcgtgggc gaggaacaga tcgtccgttt
    13561 cttgcggtcc gtgggggacg ggtcggggcc ctcgggtgag gtgcgcgtcc acctagcgcc
    13621 cgtcaacggc gagaccggcg tcgtcgtcac cgccggggcg gagccgatct cggcgctcgt
    13681 ccttgacgtg gcgaacgatc tggtccggac catccgcgtg gtggctaacc ccgagaagct
    13741 ggccgggctt cggggcatag agacgacccg atgagggtaa tctcctggac gagtgtcctg
    13801 aaccttttcg tggtgttctc gctcttcgca tgctctccag aagaagtcca ccggccggac
    13861 agggacgaga accccgagga aagcatccgc gtgggcggcg gaccgttccc cggcggggaa
    13921 gccaggacga tcgccaggac aggtggccgc gtgtctgtcg agcgggccgt gctgagtttc
    13981 gcgggagagg ctgacctcat ccacgccagg gtcgagtacg aggggtcctg ggtctggccg
    14041 cggtgctcgc tgctggaggg acccgacgtg gagaccgtgc gccggaccat cgagacccga
    14101 gagccaccaa cagcccaggg atacacgaag ccgttgtggg aggagagcct atggagggaa
    14161 cacgagagct tgtgggccga ccggagcacg atcgagaagt ccttgcagga cggcggggcg
    14221 gttctctgga ccttccacga ggatccgaag cccgaaggcg aggtggccga cccgaggcgc
    14281 acgccgttct tcgtccgctg cgccggaggg gatctccccg acgtcgtgca cgacgtggcg
    14341 cacgtcttgg gaacccccgg gacggcctct tagccatggc atcatctcgg ggcgtttcgg
    14401 cccgaagtca tgaacgccgc gagacggcaa aggcgccccg gcgagccacc ctggtattcg
    14461 acgggcgctg cggcgtctgc acccggtttg ttcgcgcaat agagcggctc gacaggaagc
    14521 aacgcataac cgcgttgcct tgccaaatgc ctggggtgcc cgcctccgtc ggcttgagtg
    14581 cggaagagtg caggaaggcc gcctgggtag tgactccagg gggataccgg tatagaggag
    14641 ccggggccgt caacctcgcc ctggcggtgg ccctgggcac gaaactcccc tacgcgttct
    14701 accggttgcc cctgataaag tgcgcgcaag accgcgccta cgactggatc gccgcgaacc
    14761 gccacagact tcccggggac gtgccgcact gctcgcaaca cccaggggag tgcgcttgag
    14821 cgaccgagga ttgcctcaat gacctcgagg agcaagggcg tttcctggcc gtggtgggtg
    14881 cagcccctgg tggtgggtgg ttcgctggcc gctttcatag cctacagcac gtgggcggtg
    14941 ttccagaccg gcggctacta cgccgatccc tacttctcgc ccttctacac gccgtgcctg
    15001 gcggcgagat gcggggaggc cgcgacgttt ccgctgttgg gcggctggtg gacgctctcg
    15061 ccggcgctgc tcgtcgcggg ccacctgcta ctactaccgc aaggcgtact accgggcctt
    15121 ctttctggat ccccctaact gctccgaggc cgctcagagg cgggagcccc gcaaagaggg
    15181 ctaccggggc gagagggcgc tgttcgtggt caacaacctc caccgctact tctggtacgc
    15241 tggcgtcctg atcgcggtcg tgctcaccta cgacacggtc gtggccttcc gtttctccga
    15301 cgggtggggg gtcggcctcg gcagtttgat cttcctgctc aacgccgcgg cgttctggct
    15361 gtactccctc tcgtgccact cgtgccggca cgccgtcggc ggacgcctca agcacttcaa
    15421 gaggcacccc cttcggtacc ggttctacaa cctcgtgagc aagctcaacc accaccatcc
    15481 tgcgttcgca tggatcagcc tgcctgcgat ggtgttcacg gacctctacg tgcggctggt
    15541 cgcctccggc gtcgtgaccg acctgcggtt cttctagccc gatgaacccg acacaaaggc
    15601 ggcacccaga tacctgtcag ccgtgcctgc gggctaagtt cgtggtcgag ctttgagcct
    15661 ccttcccgaa agtgcaccac gctcgccaag ttggggtcgg cctcgcggcg cgaaacaaac
    15721 ttacggcgag catcgtcgcc cgggccctgg tcttgcagaa ctgggtggcg ggtcccgccg
    15781 gcgcggcgtc gtttctgccc ctctacgtgc tgcggatgcc gcgcgaggaa cggatgatgc
    15841 tggagccttt cggggatagg aatcgtgcct acttgggccg cacggatcgt gtcgtaccct
    15901 gcacagggca cggaggtgcc tgagggtggg ggcgcttcga gcagaacccc ttccgttgcg
    15961 cgttctgacg tggaacctct ggtggaggtt cggcgcgtgg gagcggcgac gcgaagtcat
    16021 catcgctgtg cttgacgatc tgaggcccga tgtctgtggg ttgcaagagg tctggtcggt
    16081 tccgggagag aacctcgccg cctccctggc ccgagagctc gggatgcacc atgcctacgc
    16141 cccatcgccc gcgcccggca agtggcagcg caggatcggc gaccacggcg tcggggtcgg
    16201 caacgcggtc ctgagccgct ggccgatctc cgagtcggac gtaaggaggc ttccgatcgg
    16261 cgacgagccc gacgaaggac gcaccgttct ttacgctcgg atcgacgccc cgcatgggaa
    16321 agttcccatc ttcgtgaccc acctgaactc cggcttgggc cagtcggcgc tgcgcaaaga
    16381 gcagctcggc tcggtcgccg ggttcatgcg ggggaagggg caggacgggc acccgccgat
    16441 cctgaccgga gatttcaacg ccccgcccga cgccgacgag gtccgctgcc tggtcggcaa
    16501 ggcgccgccg ccggcgaggg gcttcgtgct ggccgacgcc tgggactacg ccaggccgct
    16561 ggagcccggc tggacgtgga accggcgcaa cccccacgtc gcggcgacgt tcgagcccga
    16621 cgcccgcatc gactacgtgt tcgtgggacg accggacggc gaaggccggg gccaggtgct
    16681 agacgtcggg ctcgtcggga cggagcccgt gggaggcgtc tgggcctcgg accactgggg
    16741 cgtactggcc gtgctgcgcg gcggtcccaa gcacgaggat cagggttcgg gtcccagtaa
    16801 ggaacccggc ggatgcccgg gataagtaaa cgcgccgaac acgacgagct tctcgaaaga
    16861 agccgtccgg attgtccgcc gatcgaggtt tcccttctcc caaatgtgag tatcagcgtc
    16921 ctgatcgttt cttcccagga ggattcgacg ggcaaggtag aggtctggac gcagttcttg
    16981 gttatcgagg atccttctac ccatggtctt tcacgacggc ctgcaagccc gaacaaaaag
    17041 cgaccagagc gcgaagaacg caaagcacgc acaaatgata ccatgtgaac cgcacggtcc
    17101 acgcgaactg agaggtgcct catgcgaccg aacaatcgca tcccgctcga gacgctgcgc
    17161 aggtctctgg ggcgagtagc ccaggacgtg gagcgtgggc actcggtggt cgtcgagcgc
    17221 aggggcgagg aggccttcgc gctggtgccg atgaggatct atcggctcct agaggaagag
    17281 aggcgggcgc tcgtcgagtc cacgcggcag gcgagggagg ccttctccca cttgtccgaa
    17341 ggcgaggtag aggagctggt tgagggagag atagaggcca cccgtagtcg ggccgcccct
    17401 tccgtcgacg gtgctgccga tcggtaagcc gtgagggtcg tcgtggacgc caacgtctct
    17461 ggggtgctga gcgcgggggg caacccggct aagatcttgg atcatgcctt cggagaggga
    17521 ccctacgact tcgagttgtg cgccccgccg cagatgttct cgaaggtcga ggaggtactc
    17581 gcgcggccca gaatagcgag tcggctgcag tggggaccgg cggagatcgg agtctactcg
    17641 aggcggctcc ggctggccgt cacggaggtc tccacagggg atcctgagaa gataccttct
    17701 tacacggaag accccgaaga cgatccgtac atccaggccg ccgtcttggg cggcgcggcc
    17761 tacgtagtta gcggagacga cgacgtgctc aggatggaag acccgccggt gccagtcctg
    17821 agtcccgctc agttcgtccg cctctggaag gcacggctcc tttgaggtga gaagagtgcg
    17881 gagtgtttgt gctcgtctca cgcggcagaa cggccgatga ctcagcttgt cttccacctc
    17941 gtgtcaacac cgcactgcta atagcaactg caaggcagca catttaccac ttctgttggt
    18001 atttgtctct ggatcagaca gtcctggctc gaatccagat ccggcagcca accgtggtca
    18061 acgagtcgtt actatcgtct cgtgagccac gccaggagct cgctacgcaa gtatatgtag
    18121 cgccgctccg tgacggcgtg gcagccccgg cgccatcctc ctgaactccg actcgcttat
    18181 cctcaagaag tccgctgcct gcttcgcgtc cataacctcc ctgccgctgg cgagcgcctc
    18241 cgcgacccgc tcgaccttct cgagtatcgc ggtggtctcc aggagtgtgt cctggtggcc
    18301 ccgcccttca gtcttccctc tccgggcccg ctgtgccgga tttcctgggt ggcttcttta
    18361 atcaggccgc ggagactctc ggagcgccgc ttttcggggc ttcgaacagt ccgaccggga
    18421 acctactccc gtcacgttcc tgtcagactc ggataggttg gccgcactga aggcagagct
    18481 ggaacaaaaa ctcctgcaaa tacaggaact agaggggcgc tcggcaggac tcgaacctgc
    18541 gaccttctga ttcgtagtca ggatcgatac gttgttggtt gtttgccata gtctagaaaa
    18601 ccgcttatct aagccgaatc tcgaaatcaa gtgttccctg ttgtttgcaa gtgttgccct
    18661 gggtaactgt caaatctctg tcagtaagac ctctctcaaa cccgcatctt gcttctgtga
    18721 tgagcttggc ggcaagcatt gtcagagaac ctcttgtcac ttcggtgctt acagccttct
    18781 ggatggggac aagccaatga gactatctct aggcagcagt gttgtccggt ggagccactc
    18841 tcctatagaa tgaagagacc tttggggctt caatctccaa cagggagggg aataggattt
    18901 gcaagattcc gtgcctccta gggcgcactg tacgaattgc ggagtcgaaa acgatccgca
    18961 gaactccttt tgcggctcat gcggagcacc gctggcatct tctcccgatg gcactaccgg
    19021 cggaacggca gttgttagcg gcagggcctt gtcgcatggc gcatggagca tggaccgtat
    19081 ccgctcgctg agcactgagc cgatgcgaga ggccttgcta ggtcttctgc ttgcggctgg
    19141 agtctcgctt ctgctagtgg tcggcttcta tatcttgctg ctgatccggt gggttatcgg
    19201 catgggggac gcacctggca tgggagggct ggtgagcttc gtcgcagcgc acggaggcgc
    19261 cttgtccgtg agggttcctc cagggcctgc tcttttggat ataggcgggt ccttgaagat
    19321 cgacccgcct atcacctcta ttgccctgct cccattcata ctactgctct tgggcagctg
    19381 gatgatatcc cgtcggctga gagcattcgt tctcttcccg ctcgttgcaa ccgtctgcta
    19441 ctcggtgatc ctcgccgtcg tagcccttct cagcaggacg acaacctccg gtgaaggctc
    19501 agaggtcacc gtctccgccg cccccttatc agccgcattt cacggactgc tcatagcagg
    19561 gctgggcaca atactaggct tagtcgccgc tcgagggcct tttctcacgg agcggacgcg
    19621 gcaagtgatg aggggagccc tggtagcggt gggcgcctct actgtccttg cgctcgttct
    19681 tgcggtgttg gtcttactag tgactttagt gacttcgggt ccggagaacc cgcttggcaa
    19741 cctccctcag gatgccggtc cgcagccaag cgataacgcg cagccgaacg gcgcgaagcc
    19801 cgatggcggc ggggtcggca acagtattag gaccctcctg acagccatag gaggcatgtt
    19861 cgccttg
//
LOCUS       DATMVF010000259        10972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_28271, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000259 DATMVF010000000
VERSION     DATMVF010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28271"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VN178_13120"
     CDS             complement(<1..387)
                     /locus_tag="VN178_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWS81954.1"
                     /translation="MAQMKAIMVEQFGEPEVLRHVDVERPSPGEEEALIEIRSAGVNY
                     ADTMRRRNQYVVPQDLPFIPGSEVAGTVVEVGEGVDDVSSGDRVVTLLGTGGYAEYAV
                     APVGSLIPLPEGLDFDEAAAIPLQGLT"
     gene            complement(509..1720)
                     /locus_tag="VN178_13125"
     CDS             complement(509..1720)
                     /locus_tag="VN178_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS81955.1"
                     /translation="MTQAGRTKDAGSSARLDPIDLLNIDDHLSAEEKEVRQRVRSFVR
                     ERITPNIQAWYDEAVFPKEIVPEFAELGLLGMHLSGYGCAGKSAVEYGLACMELEAGD
                     SGLRTFVSVQGSLAMSAIHKFGSEDQRQKWLPPMARGETIGCFGLTEPTAGSDPASMK
                     TFARKDGSDWVLNGEKRWIGLATIADVAVVWARTDEEKNPVRGFLVPTDADGFSATDI
                     TPKLSMRASIQCDISLQDVRLPEDAMLPEAKGLSGPFACLGEARYGIIWGTMGAARDC
                     YESALAYAIEREQFGRPIASFQLVQQKLANMLIEVGKGTLLALHIGRMKDAGTLRPEH
                     ISFGKLNNVREAIEIAREARTVFGGNGVTLDYPPMRHMSNLESVRTYEGTDEVHTLIL
                     GGAITGIPAFR"
     gene            1862..3007
                     /locus_tag="VN178_13130"
     CDS             1862..3007
                     /locus_tag="VN178_13130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWS81956.1"
                     /translation="MENGPKLPLEGVRVLDLSRVLAGPYATMVLGDLGADVLKVEHPE
                     RGDDTRHWGPPFAGGESAYFLSINRNKRSIGVDLKDPDGLERVEKLAAGADVVIENWR
                     RGALEKLGLGYEKLKGANPDLVYCSITGFGPGPDEERPGYDFLVQARGGVMGITGQPG
                     GEPTKVGVAIADIVCGLFASNAILAALYRRSVTGEGACIEVPLFESTLGWLANRGQEY
                     LVSGEDTGLIGNAHPSIVPYQTFDAADKPLVVAVGNNSQFANLCEVIGRTELAEDERF
                     ATNPARVANREALISELQREFGGRPADEWADKLRAVGVPSGPVNTLADVLADEHLLGS
                     GMLKGLDHPSAGHLEMLASPILIDGDRLSIRRPPPTLGQHTEEGWRG"
     gene            complement(3045..3851)
                     /locus_tag="VN178_13135"
     CDS             complement(3045..3851)
                     /locus_tag="VN178_13135"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWS81957.1"
                     /translation="MKGVAAHIFDLEQPRTEGMPIHPAHRQAGYSYLLHRRHEDEYRP
                     EETGPRTGAAGIIVCGEHTGTHIDALCHQSDALTLFGDVPVDASVQSPCGFDRLGVEE
                     IPPILAPGVLIDVAASKGAEELEPGYAVTGEDLETCCERQGVSIEAGDVALVRTGNGR
                     HWEDEERYLAGPGMDASASYWVADRQVLAVGADNMAWDVIGLRDPELGCIMPGHLILL
                     ARCGVYIIENLVLEDLAASGHHRFDFVCTPLKFVGATGSPVRPLAVIGDR"
     gene            3996..5330
                     /locus_tag="VN178_13140"
     CDS             3996..5330
                     /locus_tag="VN178_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS81958.1"
                     /translation="MERIGGEDTGMPTSIRKVVVASFIGTTIEWYDYFIFATAAALFF
                     PDVFFPGFGDTTGTILAYTTFAVGFGARPLGGLIFGHYGDKIGRKTMLVTTILIMGIA
                     TFLVGALPSYDAIGVLAPILLVLLRVLQGIGLGGEWGGAVLMAVEHSPEDQRGLNGSW
                     PQMGVPAGLVLGTGAFALVGAVSGEAAWAWRVPFLLSAILIAVGLFIRLSIYESPAFS
                     RVRESGTEARMPIVDVLRTYPRNVLLGVGSRIGIDAAFYILAVYSLTYITANLGLPES
                     VGLTAITIAALIEIFTIPAFASLSDRVGRRPLLIGGAVFMGIWTFLFFALLSTESTVL
                     IVFAAVMGLSVGHAAVYGTQGSFYAELFGTRVRYSGASFSYQISGIFGGALAPIIAAT
                     LFPIGGATLISVYIAVLCALSVLCYFLADETYRKDIYEEEPAERRLVAEQQG"
     gene            5335..7305
                     /locus_tag="VN178_13145"
     CDS             5335..7305
                     /locus_tag="VN178_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWS81959.1"
                     /translation="MKDLRLGVDVGGTFTDLVALGEGKILTAKVPSTPGDQSEGVMNA
                     IEASEVEAGAVVALAHGMTVATNALLERRGARTALVTTAGFRDVLEIARQNRPSLYDL
                     AQDRPPALVPRGLRFTVKERMGPKGELAPLESESLDAAVAAVEEAEVEAVAVCLLFAF
                     AHPEHERRVGEALREALPDVYVSLSSEVLPEFREYERFSTTTADAYLAPKLAAYLGRL
                     AERVDESGGPPPLIMQSSGGVTSVDDAVSDAAGCVLSGPAGGVVGAAYVGSLGGYEDL
                     LTFDMGGTSTDVAPILAGEAQTTTETVVAGVPIKLPMVDVHTVSAGGGSIAWADAGGA
                     LRVGPHSAGAEPGPAAYDMGGDEPAVTDANLFLGYLADGTELGGEVVLRRELSEKALA
                     ALGEKLNLSAEDVALGTVRVANAEMTRALRIISVERGLDPREFALLAFGGAGGMHACA
                     LAEELGMQTVLVPRAGGVLSALGLAISDIRRDYVSPYLSPLDEVDAEELERSFAQMEN
                     AAEGDLEAPEHTRRADLRYKGQSFELTVEADSLERLEPRFHAAHEQRYGYRMEDEAVE
                     LVNVRLISTVPVEKPELTETEAGGTAEAGEREANFDGEWMEVPVLDREALGEGSEVEG
                     PAIVEFRESTCVVRPGWRGVVDGVGTLVLEKR"
     gene            7302..8852
                     /locus_tag="VN178_13150"
     CDS             7302..8852
                     /locus_tag="VN178_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWS81960.1"
                     /translation="MSVGRLDPVTLSVLASALAGIAEEMGAVLIRGSYSSNIKERRDC
                     STALFDSEGRMVAQAEHIPVHLGAMPEAVAAIMDRDPEPGDVFAINDPYSGGTHLPDV
                     TLVSPVAYEGEIIGYAVTRAHHSDVGGMRPGSMPSDSREIFQEGLIIPPVRLVRGGDY
                     VEDVLDLLLANVRTPDLRRGDLRAQIAANNIAEERVGELIERRGKDTILDAFEEVISY
                     AEKRTREAISDLPDGEHSAEDCMEGDGITDQDITIRATVTVEGDSMKIDFSGTADAVS
                     GNINCPLPVTRSASYFALRVLLPKDILANAGTYAPLTIEAPEGSLVNATYPSAVVAGN
                     VETSNRIADTVLAALAGFAPERIPAQGQGTMNNLLIGGPGWTYYETIGGGQGASNRGS
                     GPSGVHVGMSNTLNTPIEAFEMEYPMRVERYELLYDSGGKGEHRGGDGIVRAVRVLEP
                     ASLSLLTDRRRYGPHGVDGGESGRVGRNLLGGEELPPKVSREMDEEDVVTVETPGGGG
                     YGHADGGT"
     gene            8899..9357
                     /locus_tag="VN178_13155"
     CDS             8899..9357
                     /locus_tag="VN178_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWS81961.1"
                     /translation="MKGVVEEIFVTGEGSAAMESVEEVRTVEGCGIEGDRYCAGTGFW
                     TRYGDVCEVTLIEGEDLDYIENELGIGVKNGEHRRNIVTRGIKLGDLRRKKFRVGETV
                     LEYDRPRPPCRHVQDLSERGMTRALKGRGGICARVVRAGSIRVEDAIETA"
     gene            complement(9361..10665)
                     /locus_tag="VN178_13160"
     CDS             complement(9361..10665)
                     /locus_tag="VN178_13160"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HWS81962.1"
                     /translation="MKSFLSHDRSWAYLSMLLIGVCAVALGVWARLYDLGFPSRILWD
                     EKYFPAMANKYLQGVYQFDLHPPLGKFIIAVGIALLGNEPLGWRIMPTLFGLGLLPLA
                     ALLGWYCLKERVGAILLATFFAAETILIVHSRIGVMDIFVVFFVLAAFLAALRAENGR
                     QVIFSSVLLGLAIAVKWAAFPVAVPIGYVLWRKGLLRSFVGGLWISAVVYVAVVYAGA
                     LITITTNPIQAWVWAWDWHVDAARKVTASIPHTWGSPWWSWPIMLRPIRFFYGANAEG
                     SLQVVLAIGNPLIWWSSTLAVLVSIFDIARKALARKLSADDPIIPIVLGYAFLLVPWV
                     AGTRIPYIYNYLPIYPFAILALAYWLCKVWNLWRWGPWMVVGFAACAVALTVFFLPLV
                     LAVPMSEDDIMRRVWIDYWFYYERPVPGSGCLVANPAKLCPQ"
     gene            10726..>10972
                     /locus_tag="VN178_13165"
     CDS             10726..>10972
                     /locus_tag="VN178_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81963.1"
                     /translation="MGLPDFRDSRWIPTLDAPVEGDDYITMYVILWRFRPLEGREIEF
                     ERAYGPSGEWALLFSRDDGYLGTELLRRSEDPGEYLTL"
BASE COUNT         1932 a         3584 c         3593 g         1863 t
ORIGIN      
        1 ggtcaggccc tggagcggta tggccgcggc ctcatcgaag tcgaggcctt cggggagtgg
       61 gatcaggctc cccacgggag cgacggcgta ctctgcatac ccgcctgtgc caagcaaggt
      121 gaccacccgg tcgccggagg agacatcgtc cactccctct cctacctcta ccacggtgcc
      181 cgccacctca gaacctggga taaatgggag gtcttggggg acgacgtact ggttgcggcg
      241 gcgcatggtg tcggcgtagt tgacgccggc ggagcggatc tcgatcaagg cctcctcctc
      301 gcccggcgaa ggacgctcga cgtcgacgtg gcggagcacc tcgggctcgc cgaactgctc
      361 gaccattatc gctttcatct gtgccatgtc gtcccccgat ctattcgcac cgcagcatgg
      421 cggctcgcaa tgcgccccta ccgctgccgg cgcaaacccg ttggctttcc gatactctcg
      481 atgcccgttg tacgggtttg cccctacgtt agcggaaggc cggtatgcct gtgatggcgc
      541 cgccgaggat cagggtgtgc acctcgtccg tgccctcgta ggtccttacg ctctccaggt
      601 tgctcatgtg gcgcatcggg ggatagtcga gcgtgacgcc gttgccgccg aagacggtgc
      661 gtgcttctcg cgctatctct atggcctccc tgacgttgtt gagcttgccg aagctaatgt
      721 gctcgggcct gagggtgcct gcatccttca tcctgcctat gtgcagcgcg aggagcgtac
      781 ccttgccgac ctcgatcagc atgttcgcga gcttctgctg gacgagctgg aaggatgcga
      841 tgggccgccc gaactgctcc ctctcgatag cgtatgccag cgcggattcg taacagtccc
      901 tggccgcacc catagtgccc cagatgatgc cgtacctggc ttcgcccagg caggcgaatg
      961 gtccgctgag gcccttcgct tcgggtagca tcgcgtcctc ggggaggcgg acgtcctgga
     1021 ggcttatgtc acattggatc gaagcccgca tcgagagctt gggggttata tccgtggccg
     1081 agaagccatc ggcatcggtg gggacgagga agccccggac ggggttcttc tcttcgtccg
     1141 tgcgcgccca gacgacggct acgtcggcga tggtagcgag cccaatccag cgcttctccc
     1201 cgttcaagac ccagtcggag ccgtccttcc tggcgaaggt cttcatgctc gccgggtccg
     1261 agccggcggt cggctcggtg aggccgaagc atccgatagt ctctcccctc gccatcggcg
     1321 gcaaccactt ctgtctctgg tcctccgatc cgaacttgtg gatggcgctc atggcgagcg
     1381 aaccctgcac gctaacgaag gtcctaaggc cggagtcgcc ggcctcgagc tccatgcaag
     1441 ccaggccgta ttcgaccgcg ctcttgcccg cgcaaccgta gccggagagg tgcatcccga
     1501 ggaggccgag ctcggcgaac tcgggtacga tctccttcgg gaagacggcc tcgtcgtacc
     1561 acgcttggat gttcggcgtg atcctctctc gtacgaagga acggaccctc tgcctgactt
     1621 ctttctcctc cgcggagagg tggtcgtcga tgttcaggag atctatgggg tcgaggcggg
     1681 ccgaggagcc ggcatccttg gttcttccag cctgggtcac agccatcttc tcacctctct
     1741 cggagcctca ccatcgaccc ggattattcc acagccttca gcacgctgcc ctgctgacaa
     1801 ggcgcgaagc gccgtgctgt caacctgagg cgaagccgaa gcgtgctgtt acactgggcg
     1861 catggagaat ggtccgaagt taccgctgga aggcgtcagg gttctcgacc tctcgcgggt
     1921 tctggcggga ccttacgcga cgatggtgct cggtgacctc ggcgcggacg tgttgaaggt
     1981 cgagcacccc gagcgcgggg acgacacccg ccactggggt ccgcccttcg caggtggcga
     2041 gtcggcgtat ttcctctcca tcaaccgcaa caagcgttcg atcggggtag atctgaaaga
     2101 ccctgatggg cttgagcggg tcgagaagct ggcggccggg gccgacgtcg tgatcgagaa
     2161 ctggaggcgc ggggccctgg agaagctcgg gctgggttac gagaagttga aaggggcgaa
     2221 cccggatctg gtctactgct ccatcacggg cttcggcccc gggccagacg aggagcgtcc
     2281 tggctacgac tttctggtgc aggccagggg tggggtcatg ggcatcaccg gccagcccgg
     2341 cggcgaaccg acgaaggtcg gggttgcgat cgccgacata gtgtgcggac tttttgcgtc
     2401 gaacgcgatc ctggcagccc tctatcggcg cagcgtgacc ggagagggcg cgtgcatcga
     2461 agtgcctctc ttcgaatcca ccctcggctg gctcgccaac cgcggccagg agtatctcgt
     2521 cagcggcgaa gacaccggcc tcataggcaa cgcccacccc tccatcgttc cttaccagac
     2581 cttcgatgcc gcggacaaac ccctcgtcgt ggccgtcggc aataactccc agttcgcgaa
     2641 cctgtgcgag gtcatcggtc gtaccgagct agccgaggac gaacggttcg cgaccaaccc
     2701 cgcccgcgtg gccaacagag aggcccttat ctcggagctt cagagagagt tcggcgggcg
     2761 gcccgccgac gagtgggccg acaagctccg ggccgtcggc gtccccagcg gcccggtcaa
     2821 cacgctggcc gacgtgctgg ccgacgagca cctcctcggc tcgggcatgt taaagggcct
     2881 ggatcacccc tcggccggac acctcgaaat gctcgcctcg ccgatactca tcgacggcga
     2941 tcgcctgtcc atccgccgtc ccccaccgac cctcgggcag cacaccgaag aaggctggcg
     3001 agggtagtct gaccgagtgg tgaaagcacg aaccgtgcat ccccttatcg gtcgccgatt
     3061 accgccaaag gtctgacagg agaccctgtc gccccgacga acttcaaggg cgtgcagacg
     3121 aagtcgaagc gatggtgacc cgaggcggcg agatcctcca gtaccaggtt ctcgatgatg
     3181 tagacgccgc agcgggccag gaggatcaga tgccccggca tgatgcaccc gagctcggga
     3241 tccctcagcc cgatcacgtc ccacgccatg ttgtccgccc cgaccgccag tacttgtctg
     3301 tcggcgaccc agtaggaggc actcgcatcc atgcccggtc cggcgaggta gcgttcttca
     3361 tcctcccagt ggcggccgtt gccggtccgg acgagcgcca cgtcgccggc ctcgatgctt
     3421 acgccctgcc gctcgcagca ggtctctaga tcctcccccg tgacggcata gcccggctcg
     3481 agttcctcgg cgcccttgct tgcggctacg tcgatgagga cccccggggc caagatcggg
     3541 ggtatctcct caacgccgag cctgtcgaag ccgcacgggc tctggacgct ggcgtccacg
     3601 gggacgtcac cgaagagcgt cagggcatcc gactggtgac aaagcgcgtc gatgtgggtc
     3661 cccgtgtgct ccccgcagac gatgatgccg gcggccccgg tgcgcggtcc cgtttcttca
     3721 ggcctgtact cgtcctcgtg gcggcggtgc aaaaggtagg agtagccggc ctgccgatgg
     3781 gcggggtgga tgggcatacc ctccgtgcgg ggttgctcca gatcgaagat gtgtgctgcc
     3841 acgcccttca tttcggtctc cctagtgtcc gccggccggg caagtgtggc cgagttcggc
     3901 gcaggcgtca accgcccgtg ggcctgagcg ctttgcagac cggaccgcta taatcccttc
     3961 gggacggagg gaatctcact ttcgaggagg cgaagatgga aaggatcggc ggagaagata
     4021 cggggatgcc tacttcgatc cgcaaggtgg tcgtggccag ttttatcggg acgaccatcg
     4081 agtggtacga ctactttatc ttcgccacgg ccgcggccct cttcttccca gacgtcttct
     4141 ttccgggctt cggtgacacg acggggacca tactcgccta cacgaccttc gcagtaggat
     4201 tcggggcgcg tcccctggga ggtctcatct tcggccacta cggggacaag atcgggcgca
     4261 agaccatgct cgttacgacg atcctgatca tgggcatagc cacattcctc gtgggtgccc
     4321 ttccgagcta cgacgcgata ggcgttttgg cgccgatcct gctcgtgttg ctgcgtgtcc
     4381 ttcagggtat cgggctcggc ggggagtggg gcggggccgt cctgatggcg gtcgagcact
     4441 cccccgagga ccagcgcggc ctcaacggga gctggccaca gatgggggtt cccgcggggc
     4501 tcgtgctcgg caccggcgcc ttcgccctcg tgggggcggt ctccggtgag gcggcctggg
     4561 cgtggcgcgt gccgttcctg ctgagcgcga tcctcatagc cgtgggactc ttcatccggc
     4621 tctccatcta cgagagtccg gccttctccc gggtgcgcga gtcggggacc gaggcccgca
     4681 tgccgatagt ggacgtactg cgcacctacc ccaggaacgt gcttctgggc gtgggttccc
     4741 gcatcggcat cgacgccgcc ttctacatcc tcgccgtcta ctctctgacc tacatcaccg
     4801 ccaatttggg gctccccgag agcgtcggcc tcaccgcgat caccatcgca gcgttgatcg
     4861 agatcttcac catcccggcg ttcgcctcgc tctccgacag ggtaggccgc aggccgctcc
     4921 tcatcggcgg tgcggtcttt atggggatct ggacattttt gttcttcgcc ctgctcagca
     4981 cagagtctac ggttctgatc gtgttcgccg cggtgatggg cctatcggtc ggccatgctg
     5041 cggtctacgg gacccagggg agcttctacg cggagctctt cggcacacgc gtgcgctact
     5101 cgggggcgtc tttcagctac cagatctccg gcatcttcgg cggtgccctg gcccctataa
     5161 tcgcggccac cctcttcccg attggcgggg cgaccctgat ctccgtctac atcgccgtgc
     5221 tctgcgccct tagcgtactc tgctacttcc tggccgatga gacctaccgc aaggacatct
     5281 acgaggagga acccgcggaa cggcggctcg tcgccgagca gcagggatga cctcgtgaag
     5341 gacctccgcc tcggggtcga cgtgggcggt acgttcaccg acctggtcgc actcggggaa
     5401 ggcaagattc tgaccgccaa ggtaccctcc acgcccggcg accagtcgga gggcgtgatg
     5461 aacgccatcg aagcctcgga ggtcgaggct ggcgcggtcg tcgccctcgc ccacggcatg
     5521 acggtcgcga cgaacgccct cctcgaacgc aggggcgccc gcacggccct cgttacgacc
     5581 gccggcttca gggacgtact ggagatagcc cgccagaacc gtccctcgct ctacgacctg
     5641 gcacaagacc ggccgccggc gctcgtcccc cgtgggctgc gcttcaccgt caaggaaagg
     5701 atgggtccta agggagagct ggcgccgctc gaaagtgaga gcctcgatgc cgccgtcgcc
     5761 gccgtggaag aggccgaggt agaggcggta gccgtctgcc tgctcttcgc cttcgcgcac
     5821 cccgaacacg aacggcgtgt cggcgaggcg ctgcgcgagg cgctccccga cgtgtacgtc
     5881 tccctaagca gcgaggtcct gcccgagttc cgcgagtacg agaggttctc caccacgacc
     5941 gccgacgcct acctggcccc taagctcgcc gcctacctgg ggaggctggc ggagcgggtc
     6001 gacgaatccg ggggcccgcc tccgctcata atgcagtcct cgggcggcgt aaccagcgtg
     6061 gacgatgccg tctccgacgc cgcaggctgt gtcctctcgg gccctgcggg aggcgtggtc
     6121 ggcgccgcct acgtcggttc cctcggcggt tacgaggacc tcctcacctt cgacatgggc
     6181 ggtacgagca cggacgtggc cccgatcctg gccggcgaag cccagacgac cacggagacg
     6241 gtggtcgcag gcgtccccat aaagctcccc atggtcgacg tccataccgt cagcgcgggc
     6301 ggcggctcta tagcctgggc cgacgccggc ggcgcgttgc gcgtcggtcc ccactctgcc
     6361 ggtgccgagc caggtcccgc cgcatacgac atgggcggcg atgagccggc ggtgaccgac
     6421 gccaacctct tcctcggcta cctcgccgat ggcacggagc ttggcggtga ggtcgtgctg
     6481 cggcgcgaac tctcggagaa ggccctcgcc gcgctcgggg agaagctcaa cctctcggcc
     6541 gaggacgtcg ccctgggcac cgtgcgcgta gcgaacgcgg agatgacgcg cgcgctcagg
     6601 atcatcagcg tcgagcgcgg cctggacccg cgggagttcg ccctgctcgc cttcggtggg
     6661 gcaggcggga tgcacgcctg cgccctcgcc gaggagttgg gcatgcagac cgtcctcgtc
     6721 ccgcgcgccg gcggcgtcct ctccgccctc ggcctcgcca tctcggacat ccgccgcgac
     6781 tacgtgagcc cttacctctc tcccctcgac gaagtggacg ccgaagagct cgagcgcagc
     6841 ttcgcgcaga tggagaacgc ggcagaagga gacctcgaag ctccggagca cacgcgcagg
     6901 gccgacctcc gctacaaggg ccagtctttc gagctgacag tggaggctga ctctctagag
     6961 aggctggagc ctcgcttcca cgccgcgcac gagcagcgct acggctaccg catggaggac
     7021 gaggccgtcg agctcgtgaa cgtgcgcctc atctccacgg tccccgtcga gaagcccgaa
     7081 ctgacggaga cagaggccgg gggcacagcc gaagcaggag agcgggaggc caacttcgac
     7141 ggagagtgga tggaggtgcc cgtgctggac cgcgaagcct tgggcgaggg gtccgaggta
     7201 gagggtcccg ccatagtgga gttcagggag tccacgtgcg ttgtcaggcc ggggtggcgg
     7261 ggcgtggtcg atggcgtggg aacgctcgtg ttggagaaga gatgagtgta ggaagactcg
     7321 accccgtaac cctctcggtc ctcgccagcg ctctggccgg gatcgccgag gagatggggg
     7381 ccgtcctgat ccggggctcc tactcttcga acatcaagga gaggcgcgac tgctcgacgg
     7441 ccctcttcga ttcggagggc cgcatggtcg cccaggccga acacatcccg gtccacctcg
     7501 gcgcgatgcc cgaggccgtc gcggccatca tggaccgcga ccccgagcca ggcgacgtct
     7561 tcgccatcaa cgatccttac tccggcggga cccatctgcc cgacgtaacc ctcgtctccc
     7621 ccgtcgccta cgagggtgag atcatcggtt acgcggtcac gcgggcccat cactcagacg
     7681 tcgggggcat gcgtcccggc tctatgccga gcgactcgcg tgagatcttc caggagggcc
     7741 tcatcatccc gccggtccgt ctggtgcggg gcggcgacta cgtcgaggat gtgctagatc
     7801 tgctcctcgc caacgtccgc acgcccgacc tcaggcgtgg tgacctgcgc gcccagatcg
     7861 ccgcgaacaa catcgccgaa gaacgcgtcg gggagctgat agaacgtcgc ggcaaagaca
     7921 ccatcctcga cgctttcgag gaggtgatct cctacgccga gaaacgcacc cgtgaggcta
     7981 taagtgacct ccccgacggc gagcactccg cagaagactg catggagggc gacggcataa
     8041 cggaccaaga catcaccata agggccaccg tgaccgtcga gggcgactcg atgaagatcg
     8101 acttctcggg caccgccgac gcggtcagcg gcaacataaa ctgtcccctc cccgtcaccc
     8161 gctccgcaag ctacttcgcc ctgcgcgtgc tgctccccaa ggacatactg gccaacgccg
     8221 gcacatacgc cccgctaacc atagaagccc cggagggtag cctggtcaac gcgacctacc
     8281 cttccgcggt cgtggccggg aacgtcgaga cctccaaccg catcgcggac accgtgctcg
     8341 ccgcgctcgc cggcttcgcc cccgagagga tacccgcgca gggccagggc acgatgaaca
     8401 acctgctcat aggcggtcct ggttggacct actacgagac catcggcggc ggccaggggg
     8461 cgagcaacag gggttctggt ccctcgggcg tccacgtcgg gatgagcaac accctcaaca
     8521 cccccatcga ggccttcgag atggagtatc ccatgcgcgt cgagcgctac gagctgctct
     8581 acgactcggg tgggaagggc gagcaccggg gcggcgacgg catcgtgcgc gccgtgcgcg
     8641 tactcgaacc cgccagcctc tccctgctca ccgaccgcag gcgctacggg ccgcacggcg
     8701 tcgacggcgg cgaatctggc agggtcggcc gcaacctcct gggcggcgag gagttgccgc
     8761 cgaaggtcag cagggagatg gatgaagaag acgtggttac cgtcgagacg cccggcggag
     8821 gtggttacgg gcatgccgac gggggcacgt agctgctaac ctctctcccg agtccaacta
     8881 ccggagggag cacgcaggat gaagggcgtc gtcgaggaga tctttgttac aggagaaggc
     8941 agcgcggcga tggagagcgt cgaggaggtc cgtaccgtcg aggggtgcgg catcgagggg
     9001 gaccgctact gtgcgggcac gggattctgg acgcgctacg gagacgtgtg cgaagtgacc
     9061 ctgatcgaag gcgaggacct cgactatatc gagaacgagc tgggcatcgg cgtcaagaac
     9121 ggcgagcaca ggcgtaacat cgtcacccgg ggcataaagc tgggcgacct gcgcaggaag
     9181 aagttccgcg tcggggagac ggtgctggag tacgacaggc cgcgtccgcc ctgcaggcac
     9241 gtacaggact tgagcgagcg cgggatgacg cgggccctga agggtcgagg cggaatctgt
     9301 gccagggtcg tccgggccgg tagcatcagg gtagaggacg ccatcgaaac agcgtagcgt
     9361 tcactggggg cagagcttgg cggggttcgc aacgagacac ccgctcccag gaaccggtct
     9421 ctcgtagtag aaccagtagt cgatccacac gcggcgcatg atgtcgtctt cgctcatggg
     9481 taccgccagg accagcggga ggaagaatac ggtcagcgcc acggcgcagg cggcaaagcc
     9541 gacgaccatc cagggacccc acctccaaag gttccagacc ttgcaaagcc agtaggccag
     9601 cgccaggatg gcgaaggggt agatgggcaa ataattgtag atgtacggta tcctggtgcc
     9661 ggccacccag gggacgagga gaaacgcgta gccgagcacg atcggtatga tcgggtcgtc
     9721 cgccgagagc tttctggcta aggctttgcg agcaatatcg aagatgctca cgagcacggc
     9781 cagcgtgctg ctccaccaga tcagggggtt gccgatcgcg agtactacct ggagcgatcc
     9841 ctccgcgttc gccccgtaga aaaagcgtat cggccgcagc atgatcggcc agctccacca
     9901 gggcgagccc caggtgtggg ggatggaggc tgtaaccttg cgcgcggcat cgacgtgcca
     9961 gtcccacgcc cacacccagg cctgtatggg gttcgtggtg atggtaatga gcgccccggc
    10021 atagaccacg gcgacgtaga cgacggcgga gatccagagc ccccccacaa acgatcgcaa
    10081 cagtcccttg cgccacaaga cgtagcctat gggcacggcg acggggaagg ccgcccattt
    10141 gacggcgatg gccaggccca gcaacaccga agagaagatc acctgcctgc cattctcggc
    10201 ccgcagcgcc gcaaggaagg ccgcgagcac gaagaagacg acgaagatgt ccatgacccc
    10261 gatcctggaa tggactatta atatcgtctc tgcggcgaag aaggtggcca gcagtatggc
    10321 gccaaccctc tccttgaggc aataccagcc gaggagggcg gcgagcggca agaggcccag
    10381 gccgaacaac gtaggcatga tcctccaccc cagaggctcg ttgcccaaca gagcgatgcc
    10441 aacggctatg atgaacttcc cgaggggcgg atgaaggtcg aactggtaga caccttgcaa
    10501 gtacttgttg gccatggccg ggaagtactt ctcgtcccac aaaattctgg atgggaagcc
    10561 tagatcgtac agcctggccc acaccccgag cgcgaccgca cagacgccga tgaggagcat
    10621 cgagaggtag gcccacgacc tatcatgact cagaaaagac ttcataagac tcacgttact
    10681 aggtttcgcg aacgtttgta aagcagcggt aaggctacac cccagatggg tttgccggat
    10741 tttcgggatt cgaggtggat ccccacgctt gacgcccctg tcgaagggga tgactatata
    10801 accatgtacg tcatactctg gcggttccgg cccctggagg gtcgggagat cgagttcgag
    10861 cgggcctacg gcccctccgg ggagtgggcg ctgctattca gccgggacga cggctacctc
    10921 ggcactgaac tgctccggcg ctccgaggac cccggagagt acctcaccct gg
//
LOCUS       DATMVF010000260        35196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_255013, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000260 DATMVF010000000
VERSION     DATMVF010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 35196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35196
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255013"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /gene="pgk"
                     /locus_tag="VN178_13170"
     CDS             <1..121
                     /gene="pgk"
                     /locus_tag="VN178_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWS81964.1"
                     /translation="AVNKLGLEDRMSHISTGGGASLEYVEGKELPGIAVLPDK"
     gene            136..921
                     /gene="tpiA"
                     /locus_tag="VN178_13175"
     CDS             136..921
                     /gene="tpiA"
                     /locus_tag="VN178_13175"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWS81965.1"
                     /translation="MARRPMMAANWKMNKTVREAEEYTAALLPRAADAEEDVDVVVCA
                     PHTCLHEVGRMAADFAVIAGAQNFYYEDSGAFTGEVSAPMLLDVEAGAVIVGHSERRE
                     VFGETDEMVARKTKRAVEAGILPIVCVGETKEERDSGGMWEKVSGQVERVVEEFENVG
                     GEGVVFAYEPIWAIGTGDTATPEDAQDAIGRIRDLLRETKGDDFAGSVRILYGGSVKP
                     ENAAEIMAQEDIDGALVGGASLEVESFVELIAAAQNSVSPTEQ"
     gene            943..1170
                     /gene="secG"
                     /locus_tag="VN178_13180"
     CDS             943..1170
                     /gene="secG"
                     /locus_tag="VN178_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWS81966.1"
                     /translation="MLYYIVAFFQVVFCVALVGLILLHSGKGGGLSSSLGGGMGGTFS
                     GTTIMEKNLTRLTLIVVGLFLLTNVTLFFMG"
     gene            complement(1586..1673)
                     /locus_tag="VN178_13185"
     tRNA            complement(1586..1673)
                     /locus_tag="VN178_13185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1637..1639),aa:Leu,seq:gag)
     gene            1770..2636
                     /locus_tag="VN178_13190"
     CDS             1770..2636
                     /locus_tag="VN178_13190"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWS81967.1"
                     /translation="MVWISLVGLACFVALILGKGKTRTVRGEWPVNLAHRGASALAPE
                     NTIEAFRLAIEEGAGGLELDVHMTRDRQIVVIHDATVDRTTNGTGAVSEMTLDDLRGL
                     DAGYNFSPDSGPTRPYRGRGVRVPTFGEVLEEFPGVPVNIEIKAGTPGIEEMVLGVLR
                     EADALGRALVVSTPHAIVKRFRKVSDGHVSTGASRWEIGIFYFASRLLLGRLIRPAYD
                     ALQVPLRHRGIPVVTPRFIRAAHARSVRVDAWTINQADEMRRLLDLGVDVIMTDRPGT
                     LAEVLQRRTEGS"
     gene            complement(2749..3048)
                     /locus_tag="VN178_13195"
     CDS             complement(2749..3048)
                     /locus_tag="VN178_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81968.1"
                     /translation="MSYVTIQVIWLLCIFITPPALKLYPSKLWALPFCLVTVANGLHM
                     IFFRYEYNAVLRRAMRILPYAQYFTPLRDEPRYFLPLGTAYTLFGVASIVLLMAA"
     gene            3207..4457
                     /locus_tag="VN178_13200"
     CDS             3207..4457
                     /locus_tag="VN178_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWS81969.1"
                     /translation="MIRESLVKDPSLAPEGHRKIDWVAQHAPVLNKIAGEQLADGSLR
                     GRKVAMTIHLEAKTAYLALLLSEAGAEVTVSGSNPLSTQDDVCAALAERGVRVFATHD
                     SSDEDFEGFLQKTLETGPDLIVDDGAELVGRLIEHHPDLVGNVLGASEETTTGILKLR
                     AMTAEEVLPFPVLAVNDAKMKHLFDNRYGTGHSSIVALLSNTNLFISGKAVVVMGFGW
                     VGRGLAKYADGMGARVIVCEPDPVKLLEAYSEGYEVMNSLQAAAVGDVFLTGTGNLKV
                     LGVEHFARMKDGALLANAGHYDHEFDLAALREAAVGSREVRRNITEYELPDGRRLHVL
                     ARGRLVNIAAADGHPVEIMDLTFAVQALAAHHLASHAADLAPGIQPIPGAIDDLVARA
                     KLAALGVEPETLTEEQISYQQSWR"
     gene            4665..4805
                     /locus_tag="VN178_13205"
     CDS             4665..4805
                     /locus_tag="VN178_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81970.1"
                     /translation="MTDVEIEDSVEGVLPRSRYDFNALGEVYLLDFDEDEEFEVVRPS
                     HL"
     gene            complement(4792..5229)
                     /locus_tag="VN178_13210"
     CDS             complement(4792..5229)
                     /locus_tag="VN178_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81971.1"
                     /translation="MKQSLGSLIALTLACTVAATIFGFGSEIFSWRSAYQGLGREELI
                     QATRLFVYIALGVLLAFRGGWPGVLAAIVMATAATSAEWALFPFAYSWAAVDDPAGYA
                     EKFGNVGRPSYIDWTTFDILGVGISAALAQGLRMMAHANPRGG"
     gene            complement(5410..6645)
                     /locus_tag="VN178_13215"
     CDS             complement(5410..6645)
                     /locus_tag="VN178_13215"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HWS81972.1"
                     /translation="MLRSEHVMARLYRGRLIPHRLSPDDRRTLAVAEELCDLYAAHVG
                     LPRSSLERELTVREEGLGPGLDPRRGFKIVRALSKLLEERAGWSAPTEADPYTLRTRI
                     FELAAALPEPPAAEAGLLGLPTRGDVLSQVAAETGVEDPAASMYSDRQGAQLLGEFDR
                     PSPGELVRRYNVAQVQGVLYSAREIVVDLGRGADARLVFHYVKLLGLIYDLEPLDRGH
                     RLHLDGPLSIFGGTRKYGLRLAKFLPGLLMTSPWELSANVEWKGRDTVLELDSETSGL
                     ESHYLGPKDEREADDVRGAFVRAWERAKDTGHWELERGGDVLPFPERKAALVPDFTLR
                     HTGTGEVVQLEILGFWSERNLVERVALLREAEKQGHRVLVAASERLGASQEALSGAVE
                     RGVVPFKDRLSAKAVLAAL"
     gene            complement(6645..8003)
                     /locus_tag="VN178_13220"
     CDS             complement(6645..8003)
                     /locus_tag="VN178_13220"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWS81973.1"
                     /translation="MIELFYEAGTLVATGTSSGLPEPFVWDGRTRQWRAPASAYRELV
                     LSFRAEGVPHEDRASAFEKVPLESRVAMEPRPYQKEALGAWRRDGLRGVVVLPTGAGK
                     TAVAVSAMERVGRSTLVVVPTLALLKQWYSVLQDAFGGAVTIGLLGGGYHEITPITVT
                     TYDSAYIHAERYGDRFALVVYDEVHHLPAPKNAIIAKMLLAPYALGLTATPERPDGGH
                     GLLPELVGPVVYRRSPEDLAGTYLAPFELVRIPIELTAQERFDYAQAEAVYRDFLEKH
                     RLPMRSPEDWQRFIMVASTSHSGGREALLAARQRREIREGAMRKGVTLETLLRRHWDD
                     RVIVFAKSVDEVYALSRRFLIPGVTYETPARERKEILDRFREGRYRAVIASDVLNEGV
                     DVPDAGVAVILAGSASRREYVQRLGRILRPKEGKRAILYELVTAQTGEEFTARRRKEA
                     FA"
     gene            8260..9261
                     /locus_tag="VN178_13225"
     CDS             8260..9261
                     /locus_tag="VN178_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWS81974.1"
                     /translation="MSVGDMVGQMFVVSVGGTQPDYYIEKMVRKRNIGGVILFDYNME
                     SEEQVESLTSSLQRLSMQTEPAVPLFVAVDQEGGDIASAPWVAPQPAAAEVGSRGDPE
                     EAHDIAATMGRQLLRAGINTNFAPVVDTGFGVAIGNRSYGEDPAQVARMGAAAVEGFE
                     EAGVISAAKHFPNHGPATSDSHVGLPVIRHDAKTLESHDLPPFEAAVDAGVPMVMVGH
                     LLYPAIDPHNPASLSRDAISMLRRDLGFDGVVVTDDLAMAGAKGGGTPAEAAVRAVQA
                     GADLLILSSPPQQQADAYDAVVAAVESGKIPRSRIEKSVERLLTVKEGYHSRGAGRH"
     gene            9359..10600
                     /gene="ugpC"
                     /locus_tag="VN178_13230"
     CDS             9359..10600
                     /gene="ugpC"
                     /locus_tag="VN178_13230"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HWS81975.1"
                     /translation="MAEVTMEEVTKVFGEDVVAVDKMNLDIPDGEFVVFVGPSGCGKS
                     TALRMIAGLEDISAGKVFIGDQVVNDLPPRQRDIAMVFQNYALYPHMNVRENMGFALK
                     LRKMDKSEISRRVDEAARILSIERFLDRKPKALSGGQRQRVALGRAIVREPKAFLMDE
                     PLSNLDAKLRVQMRTEIAKLHNRIGTTTIYVTHDQTEAMTMADRIVVLRDGKVQQIAT
                     PQIMYDHPDNVFVAGFIGSPAMNFLQAHLEKENGGYSVRFGGTTLSLSQEEIAEAKER
                     GHDPDQYSGKELVVGVRPEDIEDAGTEEAAAVGGAEGPNTMEVEAQVIESMGSEKYVY
                     FDVSKEQHAHLRGVAEMTDDVGGGADEETGGASAEDTTGELMVARVSPESEAREGGKI
                     RLVIDSSKIHLFDPETEEAIF"
     gene            10709..12046
                     /locus_tag="VN178_13235"
     CDS             10709..12046
                     /locus_tag="VN178_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWS81976.1"
                     /translation="MTSRGQSAVLAGRRINRRDFLKMSGAGLAGAALLGTSGCGGGGG
                     SGSGNLVFSMGPDDPNKTWTTLIDKFNKENKGSFQVTYREMPADTGQYFDKLRTEFQA
                     GGGNIDVIGGDTIWPIQFAAQGWVQDLSDKFPEPEQNKFLPAPIQASIYEDAIYGVPW
                     YEDAGLLYSRSDLLEQAGYSAPPKTWDELKEMALKTSQDTGTPNGFVFQGRNYEGGVC
                     NGLEYIRTHGGDVLSGVTSGEVVIDSPEAAAGLETYHSMITDGVAPQAVASYTETESV
                     PVFGNGEAVFLREWPGTYGDIAGGNYKVKKDQVAVSQIPVTPGNESSSTLGGWTFMIN
                     AQTDMPDEAWEFVKFMSSFESFKFIATNGGYIAARKAILDDTEVQEAIPTVKLAKDVL
                     LDYATSRPVTEFYGDMSLEMQDQFNNALTGDTPPQQACESLQNSLTSIMEQAE"
     gene            12087..13004
                     /locus_tag="VN178_13240"
     CDS             12087..13004
                     /locus_tag="VN178_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS81977.1"
                     /translation="MTTASSEKKGRGWFKEEFGNPERRTAYYMVLPSLFIIVVVAFFP
                     VLYGIVLSLTDSTVSAFGSFVGLENYTEMFQDPDFLVSLTNTVIFTVVSVALEFIIGL
                     GIALAVNRAFRGRGLVRAAILVPWAFPTVISAVMWRLMFQPGIGIFQYLAETIGIISR
                     PVLSDPTLLLIAAILIDVWKTTPFMALLLLAGLQVIPGEVYEAARVDGANVWQRFFRI
                     TLPLLKGAILVAVLFRTLDAYRVYDLFWAFGARELQSLSTFVYEGVRISQLQFGTGNA
                     AAIFIFLTSFLIAMFFIRVLGMRTSAEEE"
     gene            13008..13868
                     /locus_tag="VN178_13245"
     CDS             13008..13868
                     /locus_tag="VN178_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS81978.1"
                     /translation="MASATATKGAAGRGALTTVLFYIFLVVFVFVSVFPLLWVFKMSI
                     VTKAELFASPPTIVPQNVTGAEWAQVLGDPTFQQALVNSTIISTVTTVICLFFGSIAA
                     YAISRLRFRFKGTVMTLILAISFFPAVAIIAPLFIQFTQFGLINTYASVIITDTVFAL
                     PLTIWLLVAFFRELPVDLEDAAKVDGATTIQAFRKVIVPLAAPGVFTTAILTFIHAWN
                     EFLFANTFLFDETTQPVTVVIPNFATVYTVDYGAQAAAAVVVTVPLVILVLIFQRRIV
                     SGLTAGAVKG"
     gene            13934..14839
                     /locus_tag="VN178_13250"
     CDS             13934..14839
                     /locus_tag="VN178_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWS81979.1"
                     /translation="MRANFLATDSTVRKDSRGTPYLSLKLVDRTGSVDARMWGKLPEE
                     FLHETPSPAYVEAEGHTHEYRGTLQIKLERLRLLRPEDVSEEDFLPATELDRRALAAE
                     LESAGRELGNEHLRALFEMMVSDEDFWATFCTAPAAKTMHHARIGGLLEHSVNCMRIA
                     RALARLYPVDRDLLVFGAIFHDVGKVRELSWDKGAFAYTTEGRLLGHVVLGERLVASY
                     AATIPGFPEELRLRLSHVLISHQGETEYGSPERPKTLEALLVHLVDNLDARAAMYVET
                     TNNVAPGGWSHHENPLGRALYVPEE"
     gene            complement(14919..15107)
                     /locus_tag="VN178_13255"
     CDS             complement(14919..15107)
                     /locus_tag="VN178_13255"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWS81980.1"
                     /translation="MFGVGPQEMVIIGLIFLVIFGPGSLPKMAKDMGRFVSEARRAID
                     DFKEEIAAAGDEDEDDKR"
     gene            15213..16235
                     /locus_tag="VN178_13260"
     CDS             15213..16235
                     /locus_tag="VN178_13260"
                     /inference="COORDINATES: protein motif:HMM:NF021608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HWS81981.1"
                     /translation="MISWGAARTIAVTLASRTERGPASDFDYAGAVLATIDPLSAFTG
                     IELPQGPPGGRQLRIANRAEWIDFNIEGFGRLMEPVVQRAAAGAGDLTWAVGGVTLTA
                     QVGLLLGFLSSRVLGQYDTGPLLTPGRAGEPGKVFFLDGNIVSAAARLGVPVDGLRLW
                     IVLHEMTHALQFEGYPWLRRHLGELLESLISPLAERLGLREMLRRLSGNMRTGGRSVE
                     LLMSHSQRESFDRMQAAMSVIEGYSDYVMHHVGKNLVPHYEHLKQRMARSRAHRPPLE
                     TAVFRLTGLDVKLEQYRLGERFAEAVVRRQGMEGLNRTWERPENLPSLDEVRDPGLWM
                     SRLGPA"
     gene            16222..16566
                     /locus_tag="VN178_13265"
     CDS             16222..16566
                     /locus_tag="VN178_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81982.1"
                     /translation="MDRPEMEEEPWPLSSTLGYEFAREEEARAAASDLDRRFTQDELA
                     GLRIYRVRWNGNYLVEAAFSDGTPEARLKDAVALLGESGIPVHPDDLADYKRASDEGS
                     GLPDWLRRFFGG"
     gene            complement(16587..16880)
                     /locus_tag="VN178_13270"
     CDS             complement(16587..16880)
                     /locus_tag="VN178_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81983.1"
                     /translation="MGQGTTKFVVGATVGAVGGFAVGAFATTTTARSARQSVLGGLDV
                     SARFLGRTVVQAVDGLGSVLESGYTRIRGREKYLEHEIDELREQIARLQQRMD"
     gene            16965..18332
                     /gene="selA"
                     /locus_tag="VN178_13275"
     CDS             16965..18332
                     /gene="selA"
                     /locus_tag="VN178_13275"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HWS81984.1"
                     /translation="MMETGKWDLLRSLPAVDAVMRDPSGRTLAARHGRAPATAAVRET
                     LGGLRRRIVAGERPEVSESTVAEAAARLLSGRGLRRVVNATGVVLHTNLGRSVLSERA
                     ALAAYDAATGYSNLEYDLQSGARGSRYDHAVPLLKALTGAEDALVVNNCAGATLLALA
                     AVVESKGSEAPEVIVSRGQLIEIGGGFRIPEVLALSGARLREVGTTNRTRLSDYENAV
                     NENTGAVLWVHPSNFETVGFTESVGIRDLSSLGLPVISDVGSGALTSVGEEPRVQAIL
                     RDGADLAIFSGDKLLGGPQAGIAVGASRWVSAMRRHPLARALRADKLCLAALEATLDS
                     YLQGTAEQDLPTLRMIRTPISEIEGRATNLAEDLKSNLPNLEIDVTPSVARSGGGTLP
                     TYEIHSFAVRLGGTDPEALAAELRSSNPPVIGRVREGRLWLDVRTLLPGEEEAVIGAL
                     RDAHG"
     gene            18325..20166
                     /gene="selB"
                     /locus_tag="VN178_13280"
     CDS             18325..20166
                     /gene="selB"
                     /locus_tag="VN178_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HWS81985.1"
                     /translation="MGEAGGHRISLTIGTAGHVDHGKTTLVRYMTGTDTDRLEEEHRR
                     GISIVPGYAELDLPSGRWASLVDVPGHERFVKNMVSGATGVDAFLLVVAADDGVMPQT
                     SEHLDILRILGVEQGVVALSKVDAVDEETVELAALDVEDLLESRNMSAPVIHVSGVTG
                     EGVEDLLRALDKLAEDTADRRREDLARLPVDRSFVLKGIGVVVTGTLWSGEIRPGDVL
                     HTPTGLRPKVRGVQNHGHPAGVAYAGARTALDLTGVEASEIEAGDVLLSSPVPASRDL
                     DARVRLLESAKPLDHGVRVRLHHGTRSTNARVRMTDREELEPGARALARLRLDEPLVT
                     LRGDRFVLRSMDGVTIGGGTVLDPSATGRRPDPSWLEALESGDAATAVPLALARRPAE
                     GMTTEDLSLALEFSADEVSRAVEASAGVETVGEIHAAAGEVAAARERLLKALERRAAE
                     RPESAELTVAEARTASGLSTSLADALLAEMEGEAVAQTDTGVSLPHGGVPQELEREAK
                     ELREALRAAGPQPPTAEPTPAMRLLLKRKDAVELGDSLFAAREAAESVLEQIKTICRE
                     RGEISLAGLRDSLGTSRKYAQAWLEYSDASGVTSRTGDVRVLTRRHR"
     gene            20197..20286
                     /locus_tag="VN178_13285"
     tRNA            20197..20286
                     /locus_tag="VN178_13285"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20229..20231,aa:Sec,seq:tca)
     gene            20460..20915
                     /gene="moaC"
                     /locus_tag="VN178_13290"
     CDS             20460..20915
                     /gene="moaC"
                     /locus_tag="VN178_13290"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWS81986.1"
                     /translation="MTFSHLDSEGAARMVDVGEKAVVRRTAVAGGMVRMSSETIRLLR
                     KAALPKGDVLNTARIAGVMAAKKTQELIPLCHGLNLTSADVEFEVGQDRVSITATTRA
                     SDRTGVEMEALAAVSVAALTVYDMCKAVDKGMVIEEIKLLEKTKEPAST"
     gene            21072..21596
                     /locus_tag="VN178_13295"
     CDS             21072..21596
                     /locus_tag="VN178_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HWS81987.1"
                     /translation="MNISGRRCVVVGGGAVASRKTSKLLQAGAEVVVVSPEVLPELAG
                     MGVEIQHRTYEYGDLEGANLAITATDSREVNAAVAREAKERGVPVNVADSPSEGDFAV
                     PSTLRRGDLQVAVSTGGASPTLARRIRNELEETFGPEWAGIVEQFDTARRSGGTPGQD
                     VEEEVSRCLSRLRG"
     gene            21572..22867
                     /gene="hemA"
                     /locus_tag="VN178_13300"
     CDS             21572..22867
                     /gene="hemA"
                     /locus_tag="VN178_13300"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWS81988.1"
                     /translation="MLVAVAGMSHRSAPVEVRERIAFPPCAGRIFLRRLKDEGVVSEA
                     VLLSTCNRTEVYAVVEDEGARGRVLDLLAEDRGVERASLEQDTYWLTDAEAVRHLYRV
                     ASSLDSMVVGEGQILGQVREAYRASTEEHCAGQILNRLFHTSLRVGKKVRSETGIGDS
                     SLSVPRVAVKLAEEVFGDLTGRRALVLGAGDMSELVVKHLRDRGVADLLIANRTPQRA
                     SLLAERVGGRAVAFDALAAELPEIAVVVSSTGSGEWIVRSETVAGALATRNEPLFFID
                     IAVPRDIDPVVQTLERVYLYDIDDLQAVVERNAEGRQDAAEEGEAMISPAVLEFTGWL
                     STLHVVPLIQELRDGAEQIRRHELARALSRMELSPEEAASVERMSYSLVNKLLHGPIQ
                     EIKDRAEAGSPLESSEVRRRLMALDGLDVELHRPRRESQ"
     gene            22864..23787
                     /gene="hemC"
                     /locus_tag="VN178_13305"
     CDS             22864..23787
                     /gene="hemC"
                     /locus_tag="VN178_13305"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWS81989.1"
                     /translation="MIPENTAILGTRGSRLALAQAEECAASLRAAGIAVEIRIIRTTS
                     EHHPETPLAVIDQRDVFTRQLDEALLSGDIDLAAHSLKDVPTQPPEWVVLAAVTRRRD
                     PSDVLVSDGRHTLEDLPQGAVVATSSQRRRAQLLYHRPDLEVVGIRGNVDTRVRKMRE
                     GAADAIVLARAGLERLGLDAPHTVIPPDVMLPAVGQGALAVAARENSPLRETVREALN
                     HPPTERAVRAERNMLRTLEGGCRVPVGALAVARDEDVRLRGIVASPDGALVYRGDAVG
                     SDPDEVGERLARDLLEQGAEVVLGEVREVRR"
     gene            23837..24652
                     /locus_tag="VN178_13310"
     CDS             23837..24652
                     /locus_tag="VN178_13310"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HWS81990.1"
                     /translation="MKMFAEAIVRGVPETLHSGITSADLGRPDHTKAVAQHLRYTQAL
                     EKCGLEVTALDAAEKYPDSVFIEDTAVVTARCGILARPGAESRRGEVRGVEEELSRRY
                     ETLERIIDPGTLDGGDVLQVGDHFYIGLTSRTNLEGARQLAEILQGYGYGVSLVESRE
                     YLHLKSGVSYLGDNVLVVAGELATNGEFGVFDKIVVGPHEEYCANCIRVNDHVLVPAG
                     FEKTREQIAARGYELIELEMSEFRKVDGGLSCLSLRLPGRGGDLRRTSAGVER"
     gene            24649..26184
                     /gene="cobA"
                     /locus_tag="VN178_13315"
     CDS             24649..26184
                     /gene="cobA"
                     /locus_tag="VN178_13315"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWS81991.1"
                     /translation="MIYLVGSGPGDPGLFTLKGVRCMQEADAVVYDRLAPRALLGYAK
                     PDAEKIYVGKKPGNPTISQEEINHLLVEKGHSGMTVVRLKGGDPYIFGRGGEEALALQ
                     RAGIPFEVIPGVTSGIAAPAYAGIPVTHRNVATSVAFVTGHEDPTKGGQDVDWEKLAD
                     AADTLVLYMGVGRLEEISEGLIGAGRDPDTPVACVRWGTLPEQRTITGTLRDIADKVA
                     EAGLKPPAIIVVGDVVTLREAGLDWYERRPLFGRRVVVTRSRAQAGELSRDLERLGAE
                     VHEFPTIEITPPEDFGPLDAAIRALDSFGLIVFTSVNGVEAFFGRLQYHGLDLRAVPR
                     GAKVGAIGPATAQRVQGVGLRVDVVPEEYRAEALIEALGAHPLAGERVLIPRAKVARE
                     VLPEKLREAGAEVVVPPAYESGPSSEGKEEVSLRLQSGEIDCVTFTASSTVENFVGAF
                     AAEEAARLLDGTRVACIGPITADTARKHGIRVDTTAREYTIPGLIEAVTGLLAADPVK
                     RSE"
     gene            26186..27166
                     /gene="hemB"
                     /locus_tag="VN178_13320"
     CDS             26186..27166
                     /gene="hemB"
                     /locus_tag="VN178_13320"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWS81992.1"
                     /translation="MAFPQQRLRRTRRTAGLRGFVRETRLSPEDLVYPIFVTVGEDVR
                     NPVASMPGIFQLSINHAVAEAQRVHDLGIPAVLLFGLPEQKDEAATGAYDPEGIVQLA
                     TRAIKEAAPELLVITDVCLCEYTSHGHCGVVEKETGEVLNDVSLELLARTAASQAEAG
                     ADIVAPSDMMDGRVAAIRSELDNEGFENTPIMAYAAKYASAFYGPFREAADSAPQFGD
                     RRSYQMDPANAREALVETGLDVEEGADIVMVKPALPYLDVLRRVRETTDLPVAAYNVS
                     GEYAMVKAAAQNGWIDEQRAILEALTGIKRAGADIIITYHAPDVARWLSG"
     gene            complement(27171..27398)
                     /locus_tag="VN178_13325"
     CDS             complement(27171..27398)
                     /locus_tag="VN178_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HWS81993.1"
                     /translation="MKPIELYTTAGCPYSEAAREDLEWRGVDFIEYDVERDGEARRRM
                     LELTGGTRTVPVICEEGKPVQIGWMGRGCAV"
     gene            27627..29426
                     /locus_tag="VN178_13330"
     CDS             27627..29426
                     /locus_tag="VN178_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HWS81994.1"
                     /translation="MAIREEVTPESRNVVEMAWDPITRIVGNLGIYTKIDFDNREVVE
                     CKSTSSLFRGYSVFMKGKDPRDSHFITSRICGICGDNHAVCSVYAQNMAYGVKTPHLG
                     EWIMNLGEAAEFMFDHTIYQDNLLFVDYCEQMVRETNPSWLERAETAEAPNANIHGFH
                     TIADLMRSLNPFSGQTYVEALQMSRITREMFCLMEGRHVHPSTIYPGGVGTVPTPQLF
                     TDYLVRLMRCMDWVKKIVPISDDLFDFFYDITDGEYGKVGHRRTLLGCWGAFQNPDVV
                     NYSYEGMNDAGNGSFVTPGIVVDGELLTTNLVDINLGLRILLGSSFYDDWDQEEPFVT
                     RDPLGNPVDQRHPWNQTTLPKPQKRDFDNNYSWVMSPRWLDERTGDHLALDTGGGPLA
                     RLWATALAGLVDTGYVKATGSSVEINLPGTSTMPETSFEWKIPEQGSNTLERGRARAY
                     FIAYSAAQAVYFVEKALELVRAGDTKVFQEFEVPDEAIGCGFHEAVRGVLSHHMVIRD
                     GKIANYHPYPPTPWNGSPTDSYGTPGPYEDAVQNTPIFEENGPDNFKGIDIMRSVRSF
                     DPCLPCGVHMYLGKGKTLQKLHSPTMGFEPADK"
     gene            29486..30382
                     /locus_tag="VN178_13335"
     CDS             29486..30382
                     /locus_tag="VN178_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWS81995.1"
                     /translation="MLLDDQEPRERVGRIETLLEEIESFEDLAARAKTAQMVQTLLEL
                     YGEGLGRIVESVGRLGGEDLKDELLGDELITHLLLLHGLHPVDVKTRVLGALDEVRPY
                     LESHGGNVQFLGIGDGVARVRLEGSCDGCPSSTMTLKLAIEEAVQKAAPELEGVEAEG
                     VAEPPPKPTMAFVAAPTMRKKKRKPPEENGSSSWTTIGELDLQGGGMLAKEVSGEPVL
                     FLKLAGNLYAYRDVCPGCGTSLEQGGLRVADLTCSGCGRRYDARRAGRSLDDASQSHL
                     EPVPLLVGEDGTVKVAPPSAVG"
     gene            30390..31058
                     /locus_tag="VN178_13340"
     CDS             30390..31058
                     /locus_tag="VN178_13340"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HWS81996.1"
                     /translation="MASESDFSALSALRRFAREESAKKEATEAAAQEECCDLCGQTIP
                     PDPGHRHLMEVATREIMCVCRPCSILFDSAAASEGRYRIVPERHLNLEDFQMSDAQWE
                     SLRVPVDMAFFFYNTPAGKVVAYYPSPMGPTESLLELQTWEDLETDNPLLKDMERDVE
                     ALLVNRARGARDHFLVPIDECYKLVGLIRTRWRGFSGGREIWDDITRFFEELGRRAKT
                     VTVG"
     gene            31083..31292
                     /locus_tag="VN178_13345"
     CDS             31083..31292
                     /locus_tag="VN178_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81997.1"
                     /translation="MANIKVGKPDTKPTAPAHIRGVAGGNDPGGYDAMPGHLRDGKAT
                     AERSTGINPDKRDPIDPNSPNLSPA"
     gene            31305..31955
                     /locus_tag="VN178_13350"
     CDS             31305..31955
                     /locus_tag="VN178_13350"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HWS81998.1"
                     /translation="MPDLDFEVESADVLPYAAVPTILFRLGVQNAVGGEAIDSISLRT
                     QIRIAATQRRYDQTEQEKLRELFGEPHQWKDTLRSLLWTNTNTIVTRFSGGRVFEMPV
                     ACTYDFDVVGTKYFAALGDGEIPLEFLFSGTVFYRKDGGPLQVAQVSWEKEAKFRLPV
                     RLWREMMEHYFPNSAWLRFHKDTFDRLYDYRSRNALLTWDRTLESLLNTAESEVER"
     gene            31952..33325
                     /locus_tag="VN178_13355"
     CDS             31952..33325
                     /locus_tag="VN178_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS81999.1"
                     /translation="MTDPVEKIAAAVLYEGYVLWPYRRSAQKNQRRWTFGGVYPRAYS
                     EAEGGNDPWLMQTQCLVVGEKPTVEVKVRFLHVVERKVAKLRGAGTVGRGGFQTRPSQ
                     GAGERLEFVDELRVGGDRYLAWEEAAEREVVVPGLGLAELQTPMRTPISVPAGIEEEP
                     LAGDGGEIVGALVRTWRSLEGTVEVKAEPVREGLFRLTTRVMNTAPWCGKDREETLGQ
                     TLVSTHTILKVEGGEFVSLMDPPEDLKRLAQVCENVKTWPVLAGEEGDKHTILSSPII
                     LYDYPQIAPESPGDLFDGGEIDQLLTLSILSLTDEEKEEARATDPRACEILDRSESLT
                     EEDLMGLHGAVRGFQMLRGGEQIDPFEVEGLERPAPSNVTVGGVEVRSGSRVRLWPQA
                     GRDIFDIALSGKVAIVEKIEQDFEDRVLLAVSVEDDPGREMVDMPVLGHRFYFSPEEV
                     EPLEGER"
     gene            33322..33861
                     /locus_tag="VN178_13360"
     CDS             33322..33861
                     /locus_tag="VN178_13360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HWS82000.1"
                     /translation="MSGRILVAGIGNIFLGDDGFGCEVVRRLAEREPPEGVEIVDFGI
                     RGMDLAYALMDSYEVVVFVDAVPRGEEPGTVYLIEAEVPEEGEVALDTHGMDPVNVIR
                     LARVLGAEIPRTLVVGCEPQNFTGCTHGSQIHPYEDMLMELSEPVQRAVDEAVKLVVS
                     VVEANAPSPNSYLKPDTNS"
     gene            34002..>35196
                     /gene="hypF"
                     /locus_tag="VN178_13365"
     CDS             34002..>35196
                     /gene="hypF"
                     /locus_tag="VN178_13365"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HWS82001.1"
                     /translation="MKAPIRERWEVSVRGIVQGVGFRPFVYALARRHGLAGLVRNDAE
                     GVHVEVEGDAERLNLFLRGIEEEAPPLADVEAVSWRPLAALGEQGFRIEESREGVRRR
                     ALISPDVATCEDCLREVFDPADRRYRYPFTNCTNCGPRFTITRTVPYDRSTTTMSRFE
                     MCPECQREYDDPADRRFHAQPNACPACGPRVRLLDRFGHRLHAKPGDPILRTARMLRG
                     RAIVAVKGLGGYHLACDPFDELAVRTLRGRKVRQDKPFALMARDLAQVREFCRVGSVE
                     EALLTSPARPIVLLERLDDSGIADAVAPRQSTLGVMLAYTPLHHLLMADAGIPLVMTS
                     GNNSDEPVAYRDEEAFEQIGEIADFFLVHDRPIHMRTDDSVVRVARKETYPIRRSRGY
                     APASLA"
BASE COUNT         6743 a        10959 c        11660 g         5834 t
ORIGIN      
        1 agccgtcaac aagctcgggc tcgaagaccg gatgagccac atctcgaccg gtgggggggc
       61 ttcgctcgag tatgtagaag gcaaggagct gcctggcata gcagtcctgc cggacaagta
      121 gatggggagg agaacatggc ccgacgcccg atgatggccg cgaactggaa gatgaacaag
      181 accgtcaggg aggccgagga gtacaccgcg gccctcctgc cgcgcgccgc cgatgcagag
      241 gaagacgtgg acgtagtggt ctgcgcccca cacacctgtt tgcacgaggt gggcaggatg
      301 gccgcagatt ttgcggtcat cgcgggggcg cagaacttct actacgagga ctcgggggcg
      361 tttaccggcg aggtctcggc ccccatgctc ctcgacgtcg aggccggtgc ggtcatcgtc
      421 ggccactccg agcggcggga agtcttcggc gagaccgacg agatggtcgc cagaaagacg
      481 aagcgcgcgg tggaggccgg catcctgccc atcgtctgcg tgggggagac caaggaggaa
      541 cgcgactcag gtggtatgtg ggagaaggtc tcgggccagg tcgagcgggt ggtcgaagag
      601 ttcgaaaacg ttggcggcga gggcgtcgtc ttcgcctacg agccgatttg ggctataggg
      661 acgggcgata cggccacccc cgaggatgcg caggacgcca tcgggaggat cagggacctc
      721 ttgagagaga cgaaaggaga cgatttcgca ggtagtgtcc gtatcctcta cggtggctcc
      781 gtcaagccgg agaacgcggc cgagatcatg gcccaggaag acatcgacgg cgccctggtg
      841 ggcggagcga gcctcgaggt cgagtccttc gtcgagctga tagcggccgc gcagaactcc
      901 gtctcgccta cagagcaata gggcgtgtgg taaggtagca gcatgctgta ctacatcgtc
      961 gctttctttc aagtcgtgtt ctgcgtagcc ctggtcggcc tgatcctcct ccactcgggc
     1021 aagggaggcg ggctctcctc ctcgctcggc ggcggaatgg gcggtacgtt ctcgggcacc
     1081 accatcatgg agaagaacct gaccaggctg accctgatcg tcgtaggact cttcctgctg
     1141 accaacgtca ccctgttctt catgggatag agcatttcag cacggcgcct tcggcgactt
     1201 gtcagcacgc ttcgggcctt agcaggccct cgctttcagc aagtcagctt ctcccgttct
     1261 ggccttgccc tgccgcgcac aacgaaccgc taccaacggc ggtcgggttc ttcgcccttg
     1321 gcgaggctac ggggcgacta gctgaaatgc tgagagccgc cgaccagagg gagggggcgt
     1381 gctgacaaga gaaggcgaag catgagcgtg ctgactggct agaagaagga ctcgcccggg
     1441 gaggatgggg agaacaggcg agtccacgct aattatacac cccaaggtac ctccgggcgt
     1501 gtacaggctt acacggccac agcgccgtac gcaaggctca cgccttgtct cgacgagcca
     1561 tcagcctgaa tcgcctggaa gagactggtg tcggaggggg gactcgaacc cccaccccct
     1621 tatcggggac taggccctca acctagcgcg tctaccaatt ccgccactcc gacagggtag
     1681 cccgctgagt atacgagaga cgtatagtgg ggtcaacgcc gcacgccttc tctccactgt
     1741 gctaagttct gctgatgagc agaaggcact tggtctggat ttcgcttgtg gggttggcct
     1801 gctttgtcgc cctgatcctg ggtaagggga aaaccaggac cgtccggggt gaatggcccg
     1861 tcaacctggc acacaggggc gcttcggccc tggccccgga gaacaccatc gaggcattcc
     1921 gactcgcgat agaggaaggc gccggcggcc tggagctgga cgtccacatg acccgcgacc
     1981 ggcaaatcgt cgtgatccac gacgcgaccg tggaccggac cacgaacggg accggcgccg
     2041 tctcggagat gaccctcgat gaccttcgcg ggttggatgc cggctacaac ttcagccccg
     2101 acagcggacc tacccgtcct taccgtggca gaggcgttcg ggtgccgact ttcggcgagg
     2161 tcctcgaaga gttccccgga gttccggtca acatcgagat aaaggccggt actcctggta
     2221 tcgaggagat ggtcctcggg gtgttacgcg aggcggacgc cttggggcgc gccctcgtgg
     2281 tctcgacccc ccacgccatc gtaaagcgct tccgtaaggt ctcggacggc cacgtctcca
     2341 cgggggcctc gaggtgggag atcgggatct tctactttgc gagcaggctc cttctggggc
     2401 gtctcattcg tccggcctac gatgcattgc aggttcccct caggcacagg ggaataccgg
     2461 tcgtgacccc gcgcttcatc cgtgcggcgc atgccaggag cgtgagggta gacgcgtgga
     2521 ccataaacca ggctgacgag atgcgccgtc tgctcgacct cggcgtcgac gtaataatga
     2581 ccgaccggcc aggaacgctg gcggaggtgc tacagcgtcg gacagaaggc tcatgagcct
     2641 tctgtccgac gagctatcag ccatcagaaa gacacaaaaa gctgaaggct gactgctgaa
     2701 agttgacggc gcggatttgg gaagggtcga cccttcccaa atccgctatc acgctgccat
     2761 gagcagcacg atagatgcca cgccgaagag cgtgtaggcg gtgccaagcg gcagaaagta
     2821 cctgggctcg tcccgaaggg gcgtgaagta ctgggcgtag gggaggatac gcatcgcgcg
     2881 cctgaggacg gcgttgtact cgtagcggaa gaagatcatg tgcagcccgt tggcgacggt
     2941 gaccaggcaa aacggcaggg cccagagttt cgaaggatag agcttcaagg cagggggcgt
     3001 gatgaagatg cagagcagcc agatcacctg gatggtcacg tagctcaccc aggcggccgt
     3061 gatcctgtat ctgtgcttct cgtcgatggt gagattctaa tgtagggacc tggacgagag
     3121 catccaattt gtccggattg tagaatggtc gccacgcgga tgaggctgaa tccgtctgca
     3181 gaacagtata cacgagtagg agaaacatga tccgtgaatc gctcgtcaaa gacccctctc
     3241 tcgcaccgga agggcaccgc aagatcgatt gggtcgccca gcacgcgccg gtcttgaaca
     3301 agatcgcggg cgagcaactc gccgatggtt cgttgcgcgg ccgcaaggtc gcgatgacca
     3361 tccacctgga ggcgaagacg gcgtatctgg ccctgctcct gtccgaagcc ggggcggagg
     3421 tcaccgtctc cgggagcaac ccgctctcca cccaggacga cgtttgcgcg gccctggccg
     3481 agcggggcgt gagggtcttc gcaacccacg actcttccga cgaggatttc gagggcttcc
     3541 tgcaaaagac actcgagaca ggccctgacc tgatcgtgga cgatggcgcc gagctcgtgg
     3601 ggcgtctgat cgaacaccac cccgacctgg tcggcaacgt ccttggagcc tccgaggaga
     3661 ctaccacggg catcttgaag ctaagagcca tgaccgccga ggaagtacta cccttccctg
     3721 ttctggccgt caacgacgcc aagatgaagc acctcttcga caaccgctac ggcacgggcc
     3781 actcctcgat cgtcgccctg ctctccaata ccaacctctt catctctggg aaggcggtcg
     3841 tggtaatggg cttcggctgg gtggggcgcg gcctcgccaa gtacgccgac ggcatgggcg
     3901 cccgcgtcat cgtgtgcgag cccgaccccg tgaagctact cgaggcatac tccgaaggct
     3961 acgaggtcat gaactcgcta caggccgccg cggtggggga cgtgttcctg acgggtacgg
     4021 gcaacctcaa ggtgctcggg gtagagcact tcgcgcgtat gaaagacggc gcgcttctcg
     4081 cgaacgccgg gcactacgac cacgagttcg atctcgccgc cctgcgcgag gcggcggtag
     4141 gctcgcgtga ggtcaggcgc aacatcaccg aatacgagct ccccgacgga aggcggttgc
     4201 acgtcctggc gcgtgggcgg ctggtcaaca tcgcagccgc agacgggcac ccggtcgaga
     4261 tcatggacct caccttcgct gtacaggccc tcgctgccca ccacctcgcc agccacgcag
     4321 cagatcttgc gcccggcatc caacctatcc ccggggcgat agacgacctc gtcgcccgcg
     4381 ccaagctggc cgcactcggc gtagaacccg agaccctcac agaggagcaa atctcctacc
     4441 agcagagctg gcgttagaag gcctagatcc acggaaaccc ctgtaggggc gggtctcaga
     4501 cccgcccaat cgcgaaccga tccgctggta atatccgagg tatgaggact ataacgattc
     4561 ggtacaccgg tttccgggag tgccccaact gcgaagggcg gggtgatgac ggcacttcct
     4621 ggcgccctgc ggaatgcaag cggtgcaagg gaagaggcag actcgtgacc gacgttgaga
     4681 tcgaggactc agtggagggg gtcctgcccc gctcgcgcta cgatttcaac gccctgggcg
     4741 aggtctacct gctcgacttc gacgaagacg aagagttcga ggtcgtgcga cctagccacc
     4801 tctaggattg gcgtgggcca tcatccgcag tccttgcgcc agggcggcgg agatcccgac
     4861 ccccagaatg tcgaaggttg tccagtctat gtaagaggga cgcccgacgt taccgaactt
     4921 ctcggcgtag cctgccgggt catcgacggc ggcccacgag taggcgaacg gaaagagtgc
     4981 ccactcggcg gacgtggccg ccgtggccat gacgatagcg gcaagaaccc cgggccagcc
     5041 gccacggaac gcgagcagaa ccccgagggc gatgtagacg aacaggcggg tggcctggat
     5101 cagctcttcg cggccaagtc cttggtaggc gcttctccag gagaagatct ctgacccgaa
     5161 cccgaagatt gtggccgcca cggtgcaagc cagggtcagc gcgatcaggg atcccagact
     5221 ctgcttcata ccgagtccgt tagaaaggga gcggactggg ggtacgccga gatccttcca
     5281 tacgtcattc ccgcgaaagc gggaatccag ttgtcgtagg aggaggtccg tgcgcgaagg
     5341 cgagcttttt cgtgtcgttt gcattcaccc atactaaatt gcatgcgcag gattatattg
     5401 gtcgggctac tacagcgccg cgagtacagc cttcgccgag agccggtcct tgaaaggcac
     5461 gacgcctctt tccaccgccc cagagagggc ctcctgcgac gcgccaagcc tctccgaggc
     5521 ggcgacgagc acccggtggc cttgcttctc tgcctccctg agcagggcta cgcgctcgac
     5581 gaggttacgc tcggaccaga aaccgaggat ctcaagttgg accacctccc ctgtgcccgt
     5641 atgccgaagt gtaaagtccg ggacgagcgc cgctttccgc tcggggaagg gcagcacatc
     5701 acctcctcgt tcgagctccc agtggcccgt atccttcgcg cgctcccagg cacggacgaa
     5761 tgcaccgcgg acatcgtcgg cctcgcgttc gtccttcggt cccaggtagt gactctccag
     5821 acctgacgtc tctgaatcca gctccagaac cgtgtccctg cccttccact ctacgtttgc
     5881 cgagagttcc cagggggacg tcatcagcag gccggggagg aacttcgcca ggcgcagccc
     5941 gtacttgcgg gtgccaccga agatggagag cgggccatcg aggtggagcc tgtgaccgcg
     6001 atcgagcggt tcgaggtcgt agatcaaacc gagaagcttg acgtagtgga acacgagcct
     6061 cgcgtcggcc cctcgaccaa ggtcgacgac gatctccctc gccgagtaga gcaccccctg
     6121 tacctgggcg acgttgtagc gacggacgag ttctcccggc gagggccggt cgaactcacc
     6181 gaggagctgg gcgccctgcc tgtccgagta catcgaggcg gccgggtctt cgaccccggt
     6241 ctccgcggcg acctgagaga gcacgtcccc gcgtgtcggg agcccgagaa gacccgcctc
     6301 cgcagccggc ggttcgggaa gggcagctgc gagctcgaag atgcgggtgc gcagcgtgta
     6361 agggtcggcc tcggtcggcg cactccaacc ggcccgctcc tccaatagct tcgagagggc
     6421 gcgtacgatc ttgaatcccc tgcgggggtc gaggccgggg ccgagaccct cctccctaac
     6481 cgtcagctcc cgctccaggc tcgacctcgg tagccctacg tgggcggcgt agaggtcgca
     6541 gagttcctcg gcgacggcga gggtgcgccg gtcgtccggc gagagcctgt gagggatgag
     6601 cctgccgcgg taaaggcggg ccatgacgtg ctcgctgcgg agcactaggc gaaggcctcc
     6661 ttgcggcggc gggccgtaaa ctcttcgccg gtctgggccg tgacgagttc gtagagtatt
     6721 gcccttttgc cttccttggg gcggaggata cggccgagac gctggacgta ttcgcgacgg
     6781 gaggcgctgc cggcgagtat gacggcgacc ccggcgtcgg ggacgtcgac accttcgttc
     6841 aagacgtcgg aggctatgac ggccctgtag cggccctccc gaaagcggtc caggatctcc
     6901 ttgcgttcgc gggccggcgt ctcgtaggtt actcccggta tgaggaacct cctgctgagg
     6961 gcgtagacct catcgacgct cttggcgaag actatgacgc ggtcatccca atgtctcctg
     7021 aggagcgtct ccagggtcac gcccttccgc atcgctccct ccctgatctc ccgccgctgc
     7081 cgcgccgcga gcagcgcctc cctcccgccc gaatggctcg tggaggcgac catgatgaaa
     7141 cgctgccagt cctccggcga gcgcatcggc agtcgatgct tctccaggaa gtccctgtag
     7201 acggcctcgg cctgggcata atcgaagcgt tcctgggccg tgagctcgat tggtatgcgc
     7261 accagctcga agggggcgag gtaggtcccg gcgaggtcct cgggcgacct gcgatagacg
     7321 actggcccca caagctcggg cagcaatccg tgccctccgt cgggcctctc tggcgttgcc
     7381 gtgaggccga gggcgtaggg cgcgaggagc atcttcgcga tgatcgcgtt cttcggggcg
     7441 ggcaagtgat gcacctcgtc gtagacgacg agcgcgaaac ggtccccgta gcgctcggcg
     7501 tggatgtagg ccgagtcgta ggtggtcacc gtgatgggcg tgatctcgtg gtagccgccg
     7561 ccgagcagcc ctatcgtgac cgccccacca aaggcatcct gcaagacaga gtaccactgc
     7621 ttcagcagcg cgagggtcgg aacgacgacg agcgtgctcc gccccacgcg ctccatcgcg
     7681 ctcacggcca cggccgtctt cccggcgccg gtcggcagga cgaccacgcc gcgaagtcca
     7741 tccctgcgcc aggcgccgag ggcctccttc tggtaggggc ggggctccat ggccacgcgg
     7801 gactccagcg gaaccttctc gaacgccgaa gctcggtcct catgcggaac gccctcggcc
     7861 cgaaagctca gaaccagctc gcggtaggcg cttgccgggg cccgccactg gcgggtcctt
     7921 ccgtcccaga cgaaaggctc gggcagcccg gaggaagtcc cggtcgcgac gagggtgcct
     7981 gcttcgtaga acaactcgat cacgctccac aaagcataac cgagacttct tccgctctca
     8041 gcgataaact gcccgaaccg tgaccgaaag cagatcttga gaaggccaga gagaccattc
     8101 gaagcggaaa acgtgctccg agggttgacc ctgctgacct tactgatctg ctcactggcg
     8161 ggatgcggcg gcgcttctgg agaaggccac actgttcgta acgagaaaga ggatcccact
     8221 ggagatcgcg agaacaatga ggcatcaata gtacccagga tgagcgtcgg ggacatggtg
     8281 gggcagatgt tcgtcgtcag cgtgggcggc acgcagcccg actactacat cgagaagatg
     8341 gtgagaaagc ggaacatcgg cggtgtgatc ctcttcgact acaacatgga gagcgaggag
     8401 caggtagagt ctctgaccag ctctcttcag cggctctcca tgcagacgga acccgcggtg
     8461 ccgctcttcg ttgccgtcga ccaggagggg ggcgacatcg cctcggcccc ctgggtcgcg
     8521 ccgcagccgg cagcggccga ggttgggagc cgtggagacc ctgaggaggc acacgacatc
     8581 gccgccacga tgggccgcca gctcctgcgg gccggtatca acacgaactt tgctccagtc
     8641 gtcgacacgg gcttcggggt ggcaatcggc aaccgttctt acggggagga ccccgcacag
     8701 gtggccagga tgggggctgc ggccgtcgag ggattcgagg aggccggcgt aatctctgcg
     8761 gccaagcact tcccgaacca cgggccggcg acctcagact cacacgtggg cctgcccgtc
     8821 ataaggcacg acgcgaagac cctcgagtcc cacgatctgc cgccgttcga ggccgccgtc
     8881 gacgccgggg tgcctatggt gatggtcggg catctcctct acccggccat cgatccccac
     8941 aatcctgcaa gcctctccag agatgccata agcatgttgc gcagggatct cggctttgac
     9001 ggcgtggtgg tcaccgacga cctggccatg gccggtgcga agggcggagg aactcctgct
     9061 gaggctgcgg tccgggccgt ccaggccggg gctgacctgc tcatactctc cagtccaccg
     9121 cagcagcagg ccgacgccta cgatgccgtg gtggccgccg tcgagtcagg gaagatcccg
     9181 agatcgcgga tcgaaaagtc cgtcgagcgc ctgctcacgg tcaaggaggg ataccactcg
     9241 cgcggagcgg gccggcatta ggtagaatag tgccagatac gggggtggac gagcccgaaa
     9301 gacaatgagg gaatcctgga cgacggaccc gttcgggaga tcggaaggag gaagtgagat
     9361 ggccgaagtt accatggagg aggtaaccaa agtcttcggc gaggacgtgg tcgccgtcga
     9421 caagatgaac ctggacattc cggatgggga gtttgtcgtc ttcgtcgggc catccggttg
     9481 cgggaagtca acggctctgc gaatgatcgc cggcctcgaa gatatcagcg ctggcaaggt
     9541 atttataggg gatcaggtcg tcaacgacct accgccgcgc cagcgggata tagccatggt
     9601 cttccagaac tacgccctct atccgcacat gaacgtgcgg gagaacatgg gcttcgccct
     9661 aaagcttcgc aagatggaca agtcggagat ctcgcgccgg gtcgatgagg cagccaggat
     9721 actctcaatc gagcgtttcc tcgacaggaa gccgaaggcg ctctcgggtg gccagaggca
     9781 gcgggtggcc ctgggtcggg ccatagtccg tgagccgaag gctttcctta tggacgagcc
     9841 cctctccaac ctcgacgcca agctcagggt gcagatgagg acagagatcg ccaagctgca
     9901 caacaggatc ggcaccacga ccatctacgt cacccacgac cagaccgagg ctatgacgat
     9961 ggccgacagg atcgtcgtcc tcagggacgg caaggtgcag cagatagcga ccccccagat
    10021 catgtatgac catccggaca acgtctttgt ggcaggcttc atcgggagcc cggctatgaa
    10081 tttcctccag gcgcacctgg agaaggagaa cggcgggtac tcggtgaggt tcggcggaac
    10141 taccctctcc ctctcccagg aggagatcgc ggaggccaag gagaggggcc acgatcccga
    10201 ccagtactcg ggcaaggagc tcgtcgtggg tgttcgtccc gaggacatcg aggacgccgg
    10261 taccgaggag gcggccgccg tgggcggtgc cgagggcccg aacaccatgg aagtcgaggc
    10321 gcaggtcata gagagtatgg ggtccgagaa atacgtctac ttcgacgtga gcaaggagca
    10381 gcacgcgcac ctgcggggcg tcgcggagat gaccgacgat gtcggcggcg gcgcggacga
    10441 agagaccggc ggagcatcgg ccgaggacac caccggcgaa ctgatggtgg cccgtgtgtc
    10501 gccggagagc gaggcccgcg agggcgggaa gatacgcctg gtaatcgact cgagcaagat
    10561 ccacctcttc gacccggaga cggaggaggc gatcttttag gaggaaagtg ccattagggg
    10621 cgcgggggcc agatagggaa ggttggtagg gtaagacacg acacgtaggg gtaagcggga
    10681 aatctctagc tagaagggat gggtgcgaat gacgagcaga ggtcaatcgg cggtgctggc
    10741 tgggcggagg ataaaccgcc gcgacttcct gaagatgagc ggtgcgggac tcgcaggtgc
    10801 ggcactgctc gggacctcgg gctgcggcgg aggggggggg tcgggctcgg ggaacctcgt
    10861 cttctccatg ggtcctgatg acccgaacaa gacctggacc acgctgatag acaagttcaa
    10921 taaagagaac aaaggctcgt tccaggtcac ctaccgcgag atgcccgccg acacggggca
    10981 gtatttcgac aagctgagaa ccgagttcca ggccgggggc ggcaacatag acgtcatagg
    11041 aggcgacacc atctggccga tccagttcgc cgcgcagggc tgggtccagg acctctccga
    11101 caagttcccc gagccggagc agaacaagtt cctgcccgct cccatacagg caagtatcta
    11161 cgaagacgcg atctacgggg tgccgtggta cgaggacgcg ggcctgctct actcccgcag
    11221 cgatctcctc gagcaggccg gctactccgc gccccccaag acctgggacg aactcaagga
    11281 gatggcactc aagacctcgc aggacacggg taccccgaac ggtttcgtct tccagggaag
    11341 gaattacgag ggcggggtgt gcaacggtct ggagtacatc agaacccacg gcggcgatgt
    11401 gctctccggc gtaacgagcg gcgaggtcgt catagacagc cccgaggcgg cggcaggcct
    11461 cgagacgtat cacagcatga tcaccgacgg cgtagcaccc caagccgtgg ccagctacac
    11521 cgagactgag tcggtcccag tatttggcaa cggcgaagcg gtgttcctca gggagtggcc
    11581 gggaacatat ggggacatag ctggtggtaa ctacaaggta aagaaggacc aggtagcggt
    11641 ctcacagata cctgtgaccc ccggcaacga gagctctagc accctcggcg ggtggacctt
    11701 tatgatcaac gcgcagaccg atatgccgga tgaggcctgg gagttcgtca agttcatgtc
    11761 gagctttgag agcttcaagt tcatcgctac caacggcggc tacatagcgg ccagaaaggc
    11821 gatcctggac gacaccgagg tgcaagaagc catccctact gtcaagctgg ccaaagacgt
    11881 tcttcttgat tacgccacgt cgcggccggt gaccgagttc tacggggaca tgtcgctcga
    11941 gatgcaagat cagttcaaca acgctctgac gggcgacacc cccccgcaac aggcctgcga
    12001 gtcgctgcag aatagcctga ccagcatcat ggagcaggct gagtagggtc agccgaccgc
    12061 caggcggaca aagaaagggg tgaggcgtga cgaccgcatc tagtgagaag aaggggcgcg
    12121 gctggttcaa ggaggagttc ggcaatcccg aacgacgcac ggcctactac atggtcttgc
    12181 cgtccctatt tatcatcgtg gtcgtggcgt tcttcccggt actgtacggt atcgtgttga
    12241 gcctcaccga ctccaccgtc agcgccttcg gctcgttcgt cggcctcgag aactacaccg
    12301 agatgttcca ggaccccgac ttcctggtca gcctcaccaa caccgtgatc ttcacggtgg
    12361 tgagcgtggc gctggagttc ataatcggct tgggcatagc gctcgcggta aacagggcct
    12421 tccggggcag ggggttggtc cgggccgcaa ttctggtgcc gtgggcgttc cctacggtca
    12481 tctcggcggt catgtggcgc ctgatgttcc agcccggaat cgggattttt cagtacttgg
    12541 ccgagaccat tggcataatc agcaggccgg tactctccga cccgacgctc ctgctgatcg
    12601 cggccatcct tatcgacgtg tggaagacga cgccgtttat ggcgctcttg ctccttgcgg
    12661 gcctccaggt gattcccggc gaggtctacg aagcggcgag ggtggacggg gccaacgtgt
    12721 ggcagcgctt cttccgcata acgctgccgc tgctcaaggg cgccatactg gtcgccgtcc
    12781 tcttccgtac cctcgacgcc taccgggtct acgacctgtt ctgggcgttc ggcgccaggg
    12841 agctccagtc cttgagcacg ttcgtctacg agggcgtgag gatcagccag cttcagtttg
    12901 ggactggtaa cgcagcggcg atcttcatat tcttgacgtc gttcctcata gcgatgttct
    12961 ttatcagggt actcggcatg cgaacctcgg cggaggagga gtaagcgatg gctagcgcaa
    13021 ccgcgacaaa aggagcggcg ggccgggggg cgctcacaac ggtgctcttt tacatcttcc
    13081 tcgtagtctt cgttttcgtg agcgtgttcc cactcctgtg ggtgttcaag atgagcatcg
    13141 tcaccaaggc agagctcttc gcctcgccac caacgatcgt gccgcagaac gtaaccggtg
    13201 ccgagtgggc tcaggtcttg ggcgacccga cgttccagca ggcgctcgta aacagcacga
    13261 taatctccac ggtgaccacc gttatctgcc tcttcttcgg ctccatagca gcctacgcga
    13321 tctccaggtt gagattccgg ttcaagggca ccgtgatgac cctgatactc gcgatcagct
    13381 tcttcccagc tgtggcgatc atcgcccctc tctttatcca gttcacccag tttggcctca
    13441 taaacaccta cgcgtcggtg atcatcaccg atacggtctt cgccctaccg ctgacgatct
    13501 ggctgctggt cgcgttcttc agggagctgc ccgtcgacct cgaggacgcg gccaaggtag
    13561 atggggcgac gacgattcag gcgttccgca aggtcatcgt gccgctggcg gcacccggag
    13621 tattcaccac ggcgatcctg acgttcatcc acgcctggaa cgagttcctg ttcgccaaca
    13681 ctttcctctt cgacgagacc acccagccgg tgacggtcgt gatccctaac ttcgccaccg
    13741 tctacaccgt ggattacggg gcgcaggccg ccgctgcggt ggtagtgacg gtaccgctcg
    13801 tgatcctggt cctgatcttc cagcgccgca tcgtctccgg acttacggca ggggccgtca
    13861 aggggtagag ctagaaggga accccctgaa taaagagaag ttctgggcgc gcgatctgga
    13921 gccaggcacc aggttgcgcg ccaacttcct cgccacggac tcgacggtcc gcaaggacag
    13981 ccgcggcacg ccctacctct ccctgaagct ggtcgaccgc acgggctcgg tagacgcccg
    14041 catgtggggg aaactccccg aagagttcct ccacgagaca ccgagtccgg cctacgtgga
    14101 ggcggaggga cacacccacg agtaccgcgg caccctgcag ataaagcttg aacggctgcg
    14161 tctcctgcgg cccgaagacg tctcggaaga agacttcctc cctgctaccg agttggaccg
    14221 gcgggccctt gccgcggagc tcgaaagcgc cggacgggag ctcgggaacg agcacctccg
    14281 ggcgctcttc gagatgatgg tctcggatga ggatttctgg gcgacgttct gcacggcccc
    14341 ggcggccaag acgatgcacc acgccaggat aggggggctg ctagagcatt cggtaaattg
    14401 catgaggata gcccgcgcgc tggccagact gtaccccgta gaccgcgacc tgctcgtctt
    14461 cggcgccatc tttcacgatg tgggcaaggt gcgcgagctg tcctgggaca aaggggcctt
    14521 cgcgtacacc accgaaggcc ggctgctcgg ccacgtcgta ctcggggagc gactcgtggc
    14581 atcttacgcg gcgacgatcc ccggctttcc cgaggaacta cgcctgaggc tctcccacgt
    14641 cctgatctcg caccagggcg agacggagta cggatccccc gaacgcccca agaccctcga
    14701 agccctgctc gtccacctcg ttgacaacct cgacgcgagg gccgccatgt acgtcgagac
    14761 cacgaacaac gtggccccag gcggctggag ccaccacgag aacccgctcg gacgcgccct
    14821 atacgtcccg gaagaatagg tttcaggcca caggtctcag gtatttgttc ttcctgatgc
    14881 ctgtaacctg aagccgcagt ttgcggaagg aaaccgctct agcgcttgtc gtcctcatcc
    14941 tcgtcgccgg ccgcggcgat ctcttccttg aaatcgtcga tggcgcggcg ggcttcggac
    15001 acaaaacgtc ccatatcctt ggccatcttc ggcagcgagc ccgggccgaa gatcacgagg
    15061 aagatgagcc cgataatcac catctcttga ggcccgacgc cgaacatagg acagaatata
    15121 acccgcggat gcgcacctta caatccgccg cgacccgccg tccccggcag cgtatatact
    15181 cagcgcatgt acgctttcgt gagagacggc cgatgatctc ctggggcgcg gcgcggacca
    15241 tagccgtgac cctcgcctcc cgcaccgagc gggggcccgc aagcgacttc gactacgcgg
    15301 gcgccgtgct ggctaccatc gacccgctct cggcgttcac cggcatcgag ttgccgcagg
    15361 gacctcccgg cggccgccag ctgaggatcg ccaacagggc agagtggatc gacttcaaca
    15421 tcgaaggctt cggaaggctg atggaacccg tcgtgcagcg cgccgcagcc ggcgcgggcg
    15481 acctcacctg ggccgtcgga ggcgtgacgc taaccgccca ggtcgggctc ctgctcggct
    15541 ttctctcctc gcgggtcctg ggacagtacg atacgggacc gctgctcaca cctgggcggg
    15601 ccggggagcc aggcaaggtc ttcttcctgg acgggaacat agtctcggcc gcagcaaggc
    15661 tcggggtgcc cgtcgacggg ctgcggctct ggatcgtgct gcacgagatg acgcacgccc
    15721 tccagttcga gggttacccc tggctacgca ggcacctcgg ggaacttctg gagagcctga
    15781 tctcaccgct cgccgagcgg ctcggactgc gtgagatgct gcgacgcctc tcggggaaca
    15841 tgagaaccgg cggccggtcg gtggaacttc tgatgagcca ctcccagcgc gagtccttcg
    15901 acaggatgca ggccgccatg tcagtaatcg aaggctactc ggactacgta atgcaccacg
    15961 tcggcaagaa cctggtgccc cactacgaac atctgaagca gcgcatggcg aggagcaggg
    16021 cccaccgccc tcccctggag accgccgtct ttcgcctgac aggcctcgac gtcaagctgg
    16081 agcagtacag gctcggcgaa cgcttcgccg aggccgttgt caggcgccag gggatggagg
    16141 gcctgaatcg tacctgggag aggcccgaga atcttccttc actcgacgag gttcgcgatc
    16201 cgggcctctg gatgtcgagg cttggaccgg cctgagatgg aggaagagcc ctggccactc
    16261 tcctctacgc tgggctacga gttcgcccgt gaagaggagg ccagggccgc agcctcagac
    16321 ctcgaccgcc gctttacaca agacgagctc gcaggcctcc gcatctaccg cgtgcgctgg
    16381 aacggcaact acctggtaga ggccgcattc tcggacggta cccccgaagc gcgtctgaaa
    16441 gacgccgtcg ccctcctcgg cgagtccggc atcccggtac accccgacga cctcgcagat
    16501 tacaaacgcg cttcggacga agggtcaggc ctgcccgact ggctacgccg cttcttcggc
    16561 ggttgaaagc cggcgaaggc agcgtgctaa tccatccgtt gttggaggcg tgcgatctgc
    16621 tccctgagct cgtctatctc gtgttcgaga tacttctcac gcccacggat gcgggtataa
    16681 ccggactcca gaacggaccc gagaccatcc acggcctgga ccacggtcct gcccaggaaa
    16741 cgcgcggaga cgtccagccc gccgagcacc gactggcggg ctgaccgtgc ggtcgttgtg
    16801 gtcgcgaagg ctcctacggc gaaaccgccg acggctccga cggtcgcccc gaccacgaat
    16861 ttggtggttc cctggcccaa agatagcttc cttccggctc ttccgagcat cgacggcgcc
    16921 agtttagcac agcgccctgg cggcaaggtt atacttagcc gcgcatgatg gagacgggga
    16981 aatgggatct actgaggagc ctccccgcgg tagacgccgt aatgcgcgac ccctcgggac
    17041 ggacgctcgc cgcccgccat ggccgtgcgc cggcgacggc ggccgtccgt gagacgctcg
    17101 gtggcctacg gcgccggata gtcgcgggag aaagacctga ggtgtcagaa agcactgttg
    17161 ccgaagcagc cgcgaggctc ctctccggta gaggtctgag gcgcgtagtc aacgcaacag
    17221 gtgtggttct ccacactaat ttgggaagat cggtcctctc cgagcgggcg gccctggcgg
    17281 cctacgatgc ggccaccggc tattccaacc tggaatacga tctccagtcc ggtgctagag
    17341 gttcgcgcta cgatcacgcg gtgccgctcc tcaaggcgtt gaccggcgcc gaagacgcac
    17401 tcgtcgtcaa caactgtgcg ggggcgacgc ttctcgcgct cgccgcggtc gtcgagtcga
    17461 aaggctcgga ggcgcccgag gtgatcgtct ctcgcggtca actgatcgag ataggaggcg
    17521 gcttccgcat ccctgaagtg ctcgccctct ccggcgcgcg actcagagaa gtcggtacca
    17581 caaaccgtac caggctctcg gactacgaaa acgccgtaaa cgagaacacc ggcgcggtcc
    17641 tatgggtcca ccccagtaac ttcgagacgg tcggcttcac ggagtcagtg gggatccggg
    17701 atctctcgtc tctgggactc ccggtaatct ccgatgtagg cagcggagca ctaacgtccg
    17761 taggtgagga accgcgggta caggcgatcc tcagagatgg cgctgacctc gccatcttct
    17821 cgggagacaa gctcctcgga gggccgcagg cagggatagc ggttggcgct tcgcgttggg
    17881 tctccgccat gcgccgccat cccctcgccc gcgccctccg cgccgacaag ctgtgcctcg
    17941 ccgccctgga agcgaccctt gactcttacc tccagggcac ggcggagcag gatctcccga
    18001 ccctcaggat gatacgaact ccaatctccg agatagaagg cagggccacc aacctcgccg
    18061 aggacctgaa gagcaactta cccaacctcg agatagacgt gacgccctct gtagcccgca
    18121 gcggcggggg cacattgcca acttacgaga ttcattcctt cgccgtacgg ctgggcggga
    18181 ccgacccgga agccctggcc gcggagttgc gctcctcgaa ccctcccgtg attggccgtg
    18241 tgcgcgaggg gaggttatgg ctggacgtgc ggacgctcct gccgggcgag gaagaggctg
    18301 tgatcggggc actcagggac gcgcatgggt gaggcaggcg ggcacaggat ctccctaacc
    18361 atagggacgg cggggcatgt ggaccacggc aagaccacgc tcgtcaggta catgacgggc
    18421 acggataccg accgtctgga agaggaacac cgcaggggca tctccatcgt gccggggtac
    18481 gcggagctcg atctaccaag tggaaggtgg gcgagcctgg tcgacgtgcc cggccacgag
    18541 cgcttcgtca agaacatggt ctcgggggca acgggggtcg atgccttcct gctcgtcgtc
    18601 gccgccgacg acggggtcat gccccagacc agcgagcacc tcgacatcct gcggattctc
    18661 ggcgtcgagc agggggtcgt agcgctctct aaagtcgatg ccgtagacga ggagaccgta
    18721 gagctggccg ccctggacgt cgaggacctg ctcgagtcga ggaacatgag cgcgcctgtg
    18781 atccatgtca gcggcgttac cggcgagggc gtggaggatc tgctacgggc gctcgacaag
    18841 ttggccgagg atacagcgga ccgtcgaagg gaagatctgg cacggctacc cgtcgaccgt
    18901 tctttcgtgc taaagggcat aggggtcgtt gtaacgggta cgttgtggag cggggagatt
    18961 cgtcctggcg atgttctgca tacccctacg ggcctgcgcc cgaaggtgcg tggcgtacag
    19021 aaccacggtc accccgccgg ggtcgcctac gccggggccc gtacggccct cgacctcacg
    19081 ggcgtcgaag cctcggagat cgaagctggc gacgtcctgc tctcgtcccc cgtccccgcg
    19141 agccgcgacc tcgacgcccg tgtacgcctg ttggagagcg cgaagcccct cgaccacggc
    19201 gtcagagtga ggctgcacca cggtacccgc tcgacgaacg cccgcgttcg gatgacggac
    19261 cgtgaggagc tggagcccgg agccagggcc ctcgcccgcc tgaggctcga cgagccgcta
    19321 gtcacgctca ggggcgaccg cttcgtgctg cgctccatgg acggggtcac tatcggcggc
    19381 ggcaccgtgc tcgacccatc agcgacaggc cgtcgtcccg acccgtcatg gctcgaggcc
    19441 ctcgagagcg gcgacgccgc aacggccgtc ccactcgccc tggcccggag acccgctgag
    19501 ggcatgacca cggaagacct ctcgctcgcc ctcgaattct cggcggacga agtgtcccgc
    19561 gcggtagagg cgagcgccgg agtagagacc gtaggggaga tccacgccgc ggccggcgaa
    19621 gtcgcagcgg cacgagagcg cctcctaaaa gccctcgaac gcagggccgc cgaacgtccc
    19681 gagagtgccg agctcaccgt cgccgaagct cgcacggcgt cgggcctttc gacctccctc
    19741 gccgacgccc tgctcgcgga gatggaaggc gaagcggtcg cgcagaccga tacgggcgtc
    19801 agcctgccac acggaggagt accccaagag ctggaacgcg aggcaaagga gttacgcgag
    19861 gctttgcgag ccgcgggtcc ccaacctcct acggcggagc ccacgccagc gatgcgtctg
    19921 ctcctcaagc gcaaagacgc cgtggagctg ggagactcgc tcttcgccgc gcgcgaggcg
    19981 gccgagtccg tattggagca gataaagacc atctgcagag aaagaggtga gatctccctc
    20041 gccggactcc gcgacagcct cggaaccagc cgcaagtacg cccaggcctg gctcgagtac
    20101 tcagacgcca gcggcgtcac cagtcgcacg ggcgacgtga gagtactcac ccgccgccac
    20161 aggtaggcga tgtaataaac tctacctgta agatcaggaa tgcgtgggcc tggtggcccg
    20221 gccggtcttc aaaaccggtt tgcgaccgta atacggtcgt ggcggttcga ttcccccgca
    20281 ttccgcggaa aaagctagtc agcacgctca ggcttttagc cttcgcttgt cagcacggcg
    20341 cttcgcgcct tgtcagcaat tcagccagga ctcgcgcagg cgaaagctga agtgctgaca
    20401 agcgcccgac agggcgcttg ctgaaatgct gaattgctga gacgaccgaa gggaggcgaa
    20461 tgaccttttc tcacctcgat agcgagggcg cggcgaggat ggtcgatgtg ggggagaagg
    20521 ccgttgtacg gcgcaccgcc gtcgccgggg gaatggtacg catgtcttcg gagacgataa
    20581 ggctgttgcg gaaggctgca ttgcccaagg gcgacgtgtt gaacacggcc aggatcgccg
    20641 gggtcatggc ggccaagaag actcaggagc tcataccgct ctgtcacggg ctgaacctga
    20701 cctcagccga cgtcgagttc gaggtcggcc aggatcgggt gtcgatcacc gccacaaccc
    20761 gcgcgagcga ccgtacgggc gtagagatgg aggccctcgc tgctgttagc gtggccgcgt
    20821 tgaccgtcta cgatatgtgc aaggccgtag acaaaggtat ggtgatagag gagatcaagc
    20881 tactcgagaa aaccaaggaa ccggccagta cgtgaaggct tatacggtgc ttcgcgaggg
    20941 cgcatgcaac actttgctag acaacgatac ccggttggtg gttatgctag actttcggac
    21001 taatccacgg gccattctct aggtcaggag cagagatcgg cggcgagaat accgttcttt
    21061 accccatatt cctgaacatc tccggcaggc gttgcgtggt tgttgggggt ggggcggtgg
    21121 cgtccaggaa gaccagcaag ctgttacaag cgggcgccga ggtcgttgta gtctcacccg
    21181 aggtccttcc ggagcttgcg ggaatgggtg tggagatcca acaccgcacc tacgagtatg
    21241 gcgacctcga aggagctaac ctggcgatta ctgcgacgga ctcgcgggag gtcaacgcgg
    21301 ccgtcgcaag ggaagcgaag gaacgcgggg ttccggtaaa cgtcgcggac agtccttctg
    21361 aaggagattt cgccgtaccc tcgaccctga gaagaggtga ccttcaggtc gcggtctcga
    21421 ccggcggcgc gtcgcccacg ctggcgcgta ggatcaggaa cgagttggag gagactttcg
    21481 gtccggagtg ggccggtatc gtggagcagt tcgacacggc ccgccgcagc ggtgggacgc
    21541 cgggtcagga cgtcgaagag gaggtgagcc ggtgcttgtc gcggttgcgg ggatgagcca
    21601 ccgctcggcg ccggtcgagg tgcgcgagcg catcgctttc ccgccgtgcg ccggccggat
    21661 cttcctcagg cgcctcaagg acgagggcgt cgtctccgaa gccgtcctgc tctcgacctg
    21721 caacaggaca gaggtctacg ccgtcgtcga ggacgagggc gcgaggggac gggtgctgga
    21781 cctgctcgcc gaagacaggg gcgtcgagcg ggcgtcgctc gagcaggaca cgtactggtt
    21841 gacagacgcc gaggccgtga ggcacctgta cagggtcgcc tcttcactcg actccatggt
    21901 cgtcggggag gggcagatcc tcggccaggt ccgcgaggcg tacagggcgt ccaccgagga
    21961 gcattgcgcg ggccagatcc tcaaccggct cttccacacc tcgctcaggg tcggcaagaa
    22021 ggtccgctcc gagacgggga taggggatag ctccctctcc gtgccgcgcg tcgccgtgaa
    22081 gctcgcggag gaggtcttcg gggatctcac aggaaggcgg gccctggtcc tcggcgcagg
    22141 tgacatgagc gagctcgtgg tcaagcatct cagagacagg ggtgtagccg acctcctgat
    22201 agccaaccgt accccccagc gtgccagtct cctcgccgag cgcgtggggg gcagggccgt
    22261 cgcgttcgac gccctcgcgg ccgagctacc agagatagcc gtcgtcgtga gctccacagg
    22321 ctcgggtgag tggatcgtcc ggagcgagac ggtcgcgggg gcgcttgcga cgaggaatga
    22381 gccgttgttc tttatcgaca tcgcggtgcc gagagacata gaccccgtgg tccagaccct
    22441 ggagagggtc tacctctacg acatagacga cctccaggcc gtcgtcgagc gcaacgcgga
    22501 gggccgtcag gacgcggcag aggaggggga ggccatgatc tcccccgccg tcctggagtt
    22561 cacgggctgg ctctcgacgc tgcacgtggt cccgctcata caggaactcc gcgacggggc
    22621 agagcagatt cggcgtcacg agctcgcccg cgccctctcc aggatggaac tctcgcccga
    22681 agaggccgct tccgtcgagc gcatgagcta ctccctcgtc aacaagttgc tccacgggcc
    22741 gatccaggag ataaaggacc gcgcggaggc cggctcccct ctcgagagct ccgaagtcag
    22801 gcgccgcctg atggccctgg acggcctcga cgtggagctg caccgccccc gccgcgaatc
    22861 ccagtgatcc cggagaacac cgctatcctc ggtacccgcg gttccaggct ggccctcgcc
    22921 caggccgaag agtgcgcagc cagcctgcgc gccgccggaa tagcggtaga gatccgtatc
    22981 atcaggacga ccagcgagca ccaccccgag actcctctcg ccgtcatcga ccagcgagac
    23041 gtcttcaccc gccagctcga cgaggccctc ctttcgggcg atatcgacct cgccgcgcac
    23101 tcgctgaagg acgtaccgac ccagccgccg gagtgggtcg tgctcgcggc cgtcacccgc
    23161 cgccgcgatc cctccgacgt tctcgtctcg gatgggcgcc atactttgga agatctgccg
    23221 cagggggcag tcgtggccac atccagccag cgccggaggg cgcaactcct ttaccacagg
    23281 ccggatctgg aggtagtcgg cataaggggc aacgtggata cgcgcgtgcg caagatgcgc
    23341 gagggcgcgg ccgacgcgat cgtgctggca agggccggcc tcgagcggct cggcctcgac
    23401 gccccgcaca ccgtaatccc gccggacgtg atgctccctg ccgtcgggca aggtgccctg
    23461 gccgtcgccg ccagggagaa cagccccctg cgcgagaccg tacgagaagc tctaaatcac
    23521 ccgccgacag agagagccgt ccgggcggag cggaacatgc tgcggacgct ggagggtggc
    23581 tgccgcgtgc ccgtcggtgc attggccgtc gcccgggatg aggatgtccg gctgcgcgga
    23641 atcgtggcgt caccggacgg ggcgttggta taccggggtg atgcggtggg atccgaccct
    23701 gacgaagtcg gggaacggtt ggcgcgggat ctcctggagc agggtgcaga ggtcgtgctc
    23761 ggggaggttc gggaggtgag gagataggtg ttgcgcccgg gatcgcgcca ccgtctcccg
    23821 gaagctgccc cggggtatga agatgttcgc tgaggccatc gtccggggtg tcccggagac
    23881 gctccactcc gggataacgt ccgcggatct ggggagaccg gatcacacga aggctgtggc
    23941 gcagcacctc cggtataccc aggcactcga gaagtgcggg ctggaggtga ccgcgctcga
    24001 cgccgccgag aagtatccgg attcggtctt catcgaagac acggcggtcg tgacggcccg
    24061 gtgtgggatc cttgcccgac ccggggcgga aagcaggcgg ggggaagttc gcggtgttga
    24121 agaggagtta tccagacgct acgagaccct cgaacgcatc atcgacccgg gcacgctgga
    24181 cggaggggac gtcctgcagg tcggggatca cttctacata ggtctcacga gcaggacgaa
    24241 tctggaggga gccaggcaac tcgccgagat cctgcagggg tatggttacg gtgtctccct
    24301 cgtggagtcg cgcgagtacc tgcacctcaa gagtggggtg tcttacctgg gggacaacgt
    24361 tctggtagtg gccggggagc tggcaacgaa cggagagttc ggggtgttcg acaagatcgt
    24421 tgtaggcccg cacgaggaat actgtgccaa ctgcatccgg gtcaacgatc acgttctcgt
    24481 gcccgccggt ttcgagaaga cgagggagca gatcgccgcc agaggttatg agctcatcga
    24541 gctcgaaatg tccgagttca ggaaggtcga cggcgggttg agttgcctct cactgcgatt
    24601 gccgggacgg ggcggtgact tgcgccggac gtcggcggga gtcgagcggt gatctacctg
    24661 gtcggcagcg gacccgggga ccccggcctc tttaccctca agggcgtgcg gtgcatgcag
    24721 gaggccgacg ccgtcgtcta cgacaggctc gcgccaagag cgctgctcgg gtacgcgaag
    24781 cctgatgccg agaagatcta cgtcggcaag aagccgggta atcccacgat ctctcaggaa
    24841 gagataaacc atctcctcgt cgagaagggc cactccggga tgacggtcgt gcgcctgaag
    24901 ggcggcgacc cttacatctt cggccgtggc ggcgaagagg cgctcgcgct gcaaagggcc
    24961 ggaataccct tcgaggtcat ccccggcgtg acgagtggta tcgccgcccc cgcatacgcc
    25021 ggcatacccg tcacccacag gaacgtcgcg accagcgtcg cgttcgtcac gggacatgag
    25081 gacccgacga aaggcggcca ggacgtcgac tgggaaaaac tggccgacgc cgccgacacg
    25141 ctcgtcctgt acatgggagt gggccggctg gaggagatct cggaaggtct catcggggca
    25201 ggcagggacc cggatacgcc ggtcgcctgc gtccggtggg gcaccttacc cgaacagcga
    25261 accatcacgg gaaccctccg ggacatcgcg gataaggtcg ccgaggccgg gctgaagccg
    25321 ccggcgatta tcgtcgtcgg ggacgtcgtc accctgcgcg aagcggggct cgactggtac
    25381 gagcgacgcc cgctcttcgg gcgtagggtg gtcgttacgc ggtcgagggc gcaggccggt
    25441 gagctatcca gggatctgga gagactgggg gccgaggtcc acgagttccc gaccatagag
    25501 ataactcctc ccgaagactt cggaccactc gacgcggcga tccgtgccct cgactcgttc
    25561 ggcctcatcg tctttaccag cgtaaacggc gtcgaggcat tcttcggacg cctgcagtac
    25621 cacggactcg accttcgcgc cgtaccacgg ggcgcgaagg tgggcgccat cggtcctgcg
    25681 accgcacaga gagtacaagg ggtcgggttg cgggtcgacg tggtccccga agagtacagg
    25741 gccgaggccc tgatcgaggc cctcggcgcg caccctctgg ccggggaacg cgtcctcatc
    25801 cctcgggcga aggtggcgcg cgaggtcctg ccggagaagc tccgcgaggc tggcgccgag
    25861 gtcgtcgtcc cgcctgccta cgagtcgggt ccttcttctg aaggcaaaga ggaagtatcc
    25921 ctgcgcctgc agtcgggcga gatagactgt gtgaccttca cggcaagctc cacggtggag
    25981 aacttcgtcg gggcgttcgc cgccgaggag gcggcacgcc ttttggacgg gaccagggtc
    26041 gcgtgcatag gcccgatcac agccgatacg gcccgcaaac acggtataag ggtcgacacc
    26101 acagccaggg agtacactat ccccggacta atcgaggcgg tcacgggcct cctcgcagca
    26161 gacccggtaa agaggagcga ataggatggc attcccgcag cagaggttga gaagaacgag
    26221 gcgcacggcg ggcttgcgcg ggttcgtgcg cgagacgcgg ctctcgcccg aggatctggt
    26281 ctacccaatc ttcgtgaccg tgggagagga cgtgagaaac cctgtcgcct cgatgccagg
    26341 gatcttccag ctctcgataa accacgcggt cgccgaggcg cagcgggtgc acgacctcgg
    26401 catcccggcg gttctcctct tcggcctccc cgaacagaag gacgaggccg caaccggggc
    26461 ctacgacccc gaaggtatcg tgcaactcgc cacccgcgcc ataaaggagg ccgcgcccga
    26521 actgctcgta atcacggacg tgtgcctgtg cgagtacacg agccacggac actgcggggt
    26581 cgtcgagaag gagacgggcg aggtgctcaa cgacgtcagc ctcgagctcc tggcccggac
    26641 ggcggcctcc caggccgagg caggggccga catagtcgcg ccatcggaca tgatggacgg
    26701 gcgcgtcgcg gcgatccggt ccgagctcga caacgagggc ttcgagaaca ccccgataat
    26761 ggcctacgct gccaagtacg cctcggcctt ctacggtcct ttccgcgagg ccgccgacag
    26821 cgcgccgcag ttcggtgacc gccgtagcta ccagatggac ccggctaacg cccgcgaagc
    26881 gctcgtcgag actggcctgg acgtcgagga gggagccgac atcgtaatgg tcaaacccgc
    26941 cctgccttac ctggacgtgc tgcggcgcgt ccgcgagacg acagacctac ccgtcgccgc
    27001 ctacaacgtg agcggtgagt acgcgatggt caaggccgcc gcgcagaatg gctggatcga
    27061 cgaacagcgg gccatcctcg aagcgttgac gggcatcaaa cgcgccggtg cagacataat
    27121 catcacctac cacgcccccg acgtagcccg atggttgtcc gggtgacgta tcatactgcg
    27181 catcctctgc ccatccatcc gatctggacg ggtttgcctt cttcgcagat gacggggacc
    27241 gtcctggtgc cgccggtgag ctccagcatc cgcctgcggg cttccccgtc cctctccacg
    27301 tcgtactcga tgaagtctac cccgcgccat tccaggtcct ctcgggccgc ctcgctgtag
    27361 ggacagccgg ccgtggtgta gagctcaata ggcttcattg atcctccttc ctccacgtac
    27421 gctctctgat tctatccctc gagtgaggtc ataaggaacc cgcatgtcaa cctccgcggg
    27481 tagaacgtgc gcgaaggcga cccctgaccc ctttccgccc aaatgtccgt cattcccgcg
    27541 aaagcgggac ccaagtggcg ccacaaccgc gaggagctca ccaccggcta caacccgcgc
    27601 tggcgcgcat agaggaggga tgagcagtgg ctatccgaga ggaagtgact cccgaatcca
    27661 ggaacgtcgt ggagatggcg tgggacccca tcacgcgcat cgtcggcaac ctcgggatct
    27721 acacgaagat agacttcgac aaccgcgaag tggtggagtg caagagcacc tcgtcgctgt
    27781 tccggggcta cagcgtgttc atgaagggca aggacccgcg cgattcgcac ttcatcacca
    27841 gccgtatctg cggcatctgc ggcgacaacc acgccgtctg ctcggtctac gcccagaaca
    27901 tggcctacgg ggtgaagacg ccgcacctgg gcgagtggat catgaacctg ggcgaggcgg
    27961 ccgagttcat gttcgaccac accatctacc aggataacct cctgttcgtg gactactgcg
    28021 agcagatggt gcgggagacg aacccgagct ggctggagcg ggcggagacc gccgaggccc
    28081 ccaacgccaa catccacggc ttccacacca tcgcagacct catgcgctcc ctcaacccgt
    28141 tctccgggca aacctacgtg gaagcgttgc agatgagccg gatcacccgc gagatgttct
    28201 gcctgatgga gggacgtcac gtccatccgt ccacgatcta ccccggcggc gtgggaaccg
    28261 tgcccacccc acagctgttc acggactacc tggtccgcct catgcggtgt atggactggg
    28321 tgaagaagat cgtcccgatc agcgacgacc tcttcgactt cttctacgac atcaccgacg
    28381 gcgaatacgg gaaggtcggc caccggcgca ccctgctcgg ttgctggggc gccttccaga
    28441 acccggacgt cgtgaactac tcctacgaag gcatgaacga cgccggcaac ggatcgtttg
    28501 tgacgccggg gatcgttgtt gacggcgaac tactaacgac gaacctggtg gacataaacc
    28561 tgggtcttcg tatcctgctc ggctcttcct tctacgacga ctgggaccag gaggagccct
    28621 tcgttacccg cgacccgctg ggcaacccgg tggatcagcg gcacccctgg aaccagacca
    28681 cgctgcccaa gccgcagaag cgagacttcg acaacaacta cagttgggtt atgagcccac
    28741 gctggctgga cgagcgcacc ggggaccacc tcgcgttgga taccggcggt gggccgctgg
    28801 ccaggctttg ggccacggcc ctggccgggc tcgtggacac cggctacgtc aaggccacgg
    28861 gcagcagcgt ggagatcaac ctgccaggga cctccaccat gcccgagacg agcttcgagt
    28921 ggaagatacc ggagcagggg tccaataccc tggagcgggg ccgggcgcgg gcatacttca
    28981 ttgcctactc cgcggcgcag gccgtctact tcgtggagaa ggccctcgag ctcgtgcgtg
    29041 cgggagatac caaggtcttc caggagttcg aggtgccgga cgaggccatc ggctgcggct
    29101 tccacgaggc ggtacggggg gtcctctcgc accacatggt gatccgagac ggcaagatcg
    29161 ccaactatca tccctacccg cccacgccgt ggaacgggtc tcccaccgac tcatacggca
    29221 ctcccggtcc ctacgaggac gcggtgcaga ataccccgat cttcgaggag aacggccccg
    29281 acaacttcaa gggcatagac ataatgcgct cggtgcgtag cttcgacccg tgcctgccct
    29341 gtggggtgca catgtacctc ggcaagggca agacgctaca gaagctgcac tccccgacca
    29401 tgggcttcga gccggcggat aaatagatgg tgaggagtaa gcgggtcatg cggtaggggc
    29461 ccgcctttta tcgggaaggg aatggatgct tctagacgac caagaaccgc gagagcgcgt
    29521 cggcaggata gagacgctac tggaggagat cgaatccttc gaagacctcg cagcccgggc
    29581 caagacggct cagatggtgc agaccctcct ggagctgtac ggcgaagggc tgggacgcat
    29641 cgtggagagc gtcggccgcc tgggtggtga ggatctgaag gacgagctcc tcggcgacga
    29701 gctgatcacg cacctcctgc tcctccacgg cctgcacccg gtggacgtaa agacgcgggt
    29761 gctcggggcc ctggatgagg tgcgccctta cctcgaatcc cacggcggta acgtacagtt
    29821 cctgggtata ggcgacggtg tggcacgggt gcggctggaa ggaagctgtg acgggtgccc
    29881 gtcctccacc atgacgctca agctcgccat agaggaggcc gtccagaaag cggcgcctga
    29941 gctggaaggc gtggaggccg aaggcgtcgc cgagccgcct cccaagccca cgatggcgtt
    30001 cgtggccgcc ccgacgatgc gcaagaagaa aaggaagccg ccggaggaga acgggtcgtc
    30061 ttcctggaca acgattggtg agttggacct gcagggcggc gggatgctgg cgaaggaggt
    30121 ctctggggag ccggtgctat tcttgaagct ggcgggcaac ctctatgcct accgtgatgt
    30181 gtgtcccgga tgcggcactt cgctggagca gggtgggttg agggtggcgg atctcacgtg
    30241 ctcgggttgc gggcggcgct acgacgcgcg ccgggcgggg aggtcgctgg acgacgcctc
    30301 gcagtcgcac ctggagcccg tcccgttgct cgtgggcgaa gacggcacgg tcaaggtcgc
    30361 cccgccgagc gcggtgggct gaaggtagta tggcttccga aagcgatttt tccgctctct
    30421 ccgcgctgcg ccggttcgcc agggaggaat cagcgaaaaa ggaggcgacc gaggccgccg
    30481 cccaggaaga atgttgcgac ctgtgcggcc agaccatacc gcccgacccc gggcatcgac
    30541 acctgatgga ggtcgcgacg cgggagatca tgtgcgtctg ccggccgtgc tccatcctct
    30601 tcgacagcgc ggcggcgagc gagggacggt acaggatcgt cccagaacgt cacctgaacc
    30661 tcgaggattt ccagatgtcc gacgcgcagt gggagagcct gcgcgtccct gtggatatgg
    30721 cgttcttctt ctacaacacc cccgccggaa aggtagtggc ctattatccc agccccatgg
    30781 ggccgaccga gtcgctcctg gaactccaga cctgggagga tctggagacc gataaccccc
    30841 tattgaaaga catggaacga gacgtggagg cgctcctggt caatcgggcc cggggggcca
    30901 gagaccactt cctggtaccc atagacgagt gctacaagct ggtaggtctg atccgtaccc
    30961 gctggagggg cttcagcggc gggcgcgaga tctgggacga cattacccgg ttcttcgagg
    31021 agttgggcag acgggccaaa acagtgaccg taggctaaac ccacgacgag aggaggagac
    31081 ggatggcgaa cataaaggta gggaaaccag acacgaagcc caccgcgccg gcgcacatac
    31141 ggggggtcgc gggcggcaac gatcccggcg gctacgatgc catgccaggg caccttcgcg
    31201 acggcaaggc aacggccgag cgttccacgg gcatcaaccc cgacaagagg gaccccatag
    31261 acccgaacag cccgaacctg tcgccggcgt gaagaatcgc caccgtgcct gacctggatt
    31321 tcgaggtgga gagcgccgat gtgttgccct acgccgccgt cccgacgatc cttttcaggt
    31381 tgggcgtcca gaacgcggtg gggggagaag cgatcgactc catctcgttg cggacccaga
    31441 tccggatagc ggctacacag cgacgctacg accagacaga gcaggagaag ctacgggagc
    31501 tcttcggcga gccacaccag tggaaagaca cgctgcgcag cctcctctgg accaacacaa
    31561 acaccatagt gacccgattc tccggcggcc gcgtcttcga gatgcccgtg gcctgtacct
    31621 acgacttcga cgtggtgggg accaagtatt tcgccgccct gggagacggg gagataccgc
    31681 tggagttcct gttcagcggc acggtgttct accgcaagga cggcggaccc ttgcaggtcg
    31741 cccaggtctc ctgggagaaa gaggcgaagt tccggttgcc ggtccggctg tggagggaga
    31801 tgatggagca ctacttcccg aacagcgcct ggctccgctt ccacaaagac accttcgacc
    31861 ggctctacga ctataggtca cgcaacgcgc tgttgacctg ggatagaacg ttagagagct
    31921 tgctgaacac ggccgaaagt gaggtcgaac gttgacggac ccggtagaga agatcgccgc
    31981 cgccgtgctc tacgagggct acgtgctctg gccctatcgc cgctcggccc agaagaacca
    32041 gagacgctgg accttcggcg gcgtctaccc gcgcgcctac agcgaggcgg agggcggcaa
    32101 cgacccctgg ctcatgcaga cccagtgttt ggtagtcgga gaaaaaccca ccgtcgaggt
    32161 aaaggtccgc ttcctccacg tcgtggaacg caaagtcgcc aagctcaggg gagccgggac
    32221 cgtgggtagg ggcgggtttc aaacccgccc gtcccaggga gcaggggaac gcctggagtt
    32281 cgtggacgag ttgcgggttg gtggggaccg ctacctcgcg tgggaagagg ccgccgagcg
    32341 ggaggtcgtc gtgcctggcc tcgggctcgc ggagcttcag acgccgatgc gcacaccgat
    32401 ctcggttccc gccgggatcg aggaggagcc gctggcgggc gatgggggag aaatcgtcgg
    32461 ggcgctggtg cggacctggc gctccctgga aggcacggtg gaggtaaaag ccgaacccgt
    32521 gcgcgaaggt ctgttcagac tcacgacgag agttatgaac accgcgccgt ggtgcggcaa
    32581 ggaccgggag gagacgctag ggcaaacgct cgtctcgacg cataccatct tgaaggtgga
    32641 aggcggggag ttcgtctcgc tcatggaccc gccggaggat ctgaagcgcc tcgcacaagt
    32701 gtgcgagaac gtgaagacgt ggcccgtgct ggcgggggag gagggagata agcataccat
    32761 tctatcgtcc ccgatcatac tgtacgatta tccccagata gcgccggaga gccccggaga
    32821 tctcttcgac gggggggaga tagaccagct gcttaccctg agcatcctca gccttacgga
    32881 cgaggaaaag gaggaggcgc gcgctactga tccgcgggcg tgcgagatcc tggaccgcag
    32941 cgagtcgctc accgaggagg acctgatggg tctgcacggc gccgtccggg gtttccagat
    33001 gctgcgggga ggagagcaga tagacccctt cgaagtcgag ggcctggaga ggccggctcc
    33061 gtcgaacgtg acggtgggcg gggtcgaggt caggagcggg agccgggtga ggctctggcc
    33121 tcaggccggg agggacatct tcgacatcgc gctctccggg aaggtggcta tagtcgagaa
    33181 gatcgagcaa gacttcgagg accgcgttct tcttgcggtc tcggtcgagg acgatccggg
    33241 cagggagatg gtggacatgc cggttttggg gcaccggttc tacttctcac cggaggaggt
    33301 cgagccgctg gaaggggagc ggtgagcggg cgtatcctgg tcgccggcat cggaaacatc
    33361 ttcctcggcg acgacggctt cggttgcgag gtagtgcggc ggctcgcgga gcgcgagccg
    33421 ccggaaggcg tcgagatcgt ggacttcggg atacgaggga tggatctggc ttacgcgctg
    33481 atggactctt acgaagtcgt ggtgttcgtt gatgccgttc cacgcgggga agagccaggg
    33541 acggtctacc tgatcgaggc cgaggtcccc gaggaagggg aggtagccct cgacacccac
    33601 ggcatggacc cggtgaacgt gatccgtctg gcccgcgttc tcggtgccga gatcccgcgc
    33661 accctcgtgg tcggctgcga accgcagaac ttcacaggct gtacgcatgg gtctcagatc
    33721 cacccctacg aagacatgct catggagctc agcgagccgg tacaaagggc ggtcgacgaa
    33781 gccgtgaagc ttgtagtgtc ggtggtggag gccaacgccc cgtcgcccaa cagctacctg
    33841 aaacccgata ccaactccta aaggaggtga gaccgtgaag aaactattgt tcttgggcct
    33901 gttggcgggc gttgtcgtgg tgtttatgca gaacaggcag gacctcacga ggtacctgaa
    33961 cataagggac atgtaggagt cgtgtagctc gagctggcgc catgaaagca ccgatacggg
    34021 agcggtggga ggtctcggtc cggggcatcg tgcagggcgt ggggttcagg cccttcgtct
    34081 acgccctggc gcggcggcac gggctcgccg ggctggtccg caacgacgcc gagggagtcc
    34141 acgtcgaggt ggagggcgac gccgagaggc tcaatctctt cttgcgcggg atagaagaag
    34201 aagccccacc gctcgccgac gtggaggcag tctcctggcg accgctcgca gcgctgggcg
    34261 agcaaggttt ccggatcgag gagagccggg agggcgtcag gcggcgggcg ctcatatcgc
    34321 cggatgtcgc cacctgcgaa gactgcctgc gggaggtttt cgatcccgcg gatcgccgct
    34381 accgttaccc attcaccaac tgcaccaact gcggaccgcg cttcaccata acccgcacgg
    34441 ttccttacga ccgctccacg accacgatgt cccgcttcga gatgtgcccc gaatgccaac
    34501 gcgagtacga cgatcccgcc gaccgccgct tccacgccca gcccaacgcc tgccccgcct
    34561 gcggcccgcg cgtacggctg ctggacaggt tcggccaccg gttgcacgca aaaccgggcg
    34621 accccatcct gcgcacggcc cggatgttgc ggggacgggc catcgtcgcc gtcaagggcc
    34681 tcgggggcta ccatctcgcc tgcgacccgt tcgatgagct ggcggtgagg accctcaggg
    34741 gccgcaaggt tcgccaggac aaacccttcg cgcttatggc tagggatctc gcccaagtac
    34801 gcgaattttg tcgggtagga tcagtggaag aggcactact cacctctccg gcgcgtccca
    34861 tagtgctcct cgagcgcctg gatgatagcg ggatcgccga tgcagtggcc ccgcggcaga
    34921 gcaccctggg cgtcatgctc gcctacaccc cgctgcacca cctgttgatg gccgatgcgg
    34981 gcattccgct cgtcatgacc agcggcaaca actcagacga gcccgtagcc taccgggacg
    35041 aggaggcctt cgagcagatc ggggagatcg ccgacttctt cctggttcac gaccggccca
    35101 tccacatgcg caccgacgac agcgtggtgc gggtcgctag aaaggaaacg tatcccatcc
    35161 gccgctccag gggctacgcg ccggcgtcgc tggccg
//
LOCUS       DATMVF010000261         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_678406, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000261 DATMVF010000000
VERSION     DATMVF010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678406"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VN178_13370"
     CDS             complement(<1..563)
                     /locus_tag="VN178_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWS82002.1"
                     /translation="MNGREITAAAIQMSSTPNKGENLDTAERLIRSAASSGADLIALP
                     ELWNCHGLEEVYKENAEPVPGPTTRFLGGLAQELGVYLLGGSILEGDPQSTKLHNTST
                     FFGPDGKMSAVYRKIHLFDVKAPDREYLESRTIAAGSEIVTAKAGAATVGLSVCYDVR
                     FPELYRLLALRGAEVLAVPAAFTLQTG"
     gene            complement(601..1080)
                     /locus_tag="VN178_13375"
     CDS             complement(601..1080)
                     /locus_tag="VN178_13375"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase
                     domain-containing protein"
                     /protein_id="HWS82003.1"
                     /translation="MLVYLAGPLFSMAERRFNLELTHRLEALGFEVFLPQRDGVERDR
                     PPYDRMAPEERRRAMFELDRSRILDSDVFLFVLDGRVPDEGACVELGIAYGQKYLQKS
                     EKLLVGLHTDTRAAFIGGRLNPMVRDPLDCVVDDEETLLRVLAEQRSGVEATTEWPG"
     gene            complement(1085..1996)
                     /gene="ftcD"
                     /locus_tag="VN178_13380"
     CDS             complement(1085..1996)
                     /gene="ftcD"
                     /locus_tag="VN178_13380"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HWS82004.1"
                     /translation="MRDPLFEAVPNFSEGKDDTKISRITEAVSSVPGVRILGLHSDPD
                     HNRSVITFAGEENPVLEGAIALAEACAAELDLASQSGEHPRMGVLDVLPFVPLEGATM
                     DDAVKLAHRVGEGLGSLGFPVFLYGSAATGKHRRSLPDVRRGGYEGVSSRLEDPLWKP
                     DYGPGTLDPHVGAVAVGARPFLVAFNAYLDTDDVEVARTVARRVRESGGGLPAVRALG
                     FGVGGKAQVSMNLTDLERTAIPVALEAVRSAAAEFGATVESTELVGLAPLEAVLQTAR
                     YYLGLRELDAHHVLEARLYGPSDSRSV"
     gene            complement(2003..>2638)
                     /locus_tag="VN178_13385"
     CDS             complement(2003..>2638)
                     /locus_tag="VN178_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWS82005.1"
                     /translation="WREPDEGIWEVRGGRRQFVSSKLLSWVALERAGRIARQRGLPSG
                     DGRWMAERDAIYEEVMEKGWNPEKESFVQYYGSDALDASLLMMPLVKFVGPTDPRWLS
                     TLDCIQQELAHDTLVKRYRPEDAAPDGLEGDEGSFSLCSFWLVECLTQAGRLDEARLA
                     LEKMFSYANHLGLYAEELGSSGEALGNFPQALTHLALISAAVHLDRALGAR"
BASE COUNT          443 a          853 c          867 g          475 t
ORIGIN      
        1 ttgcccgtct ggagcgtgaa ggcggcaggg acggcgagca cctcggcgcc gcgcaacgcg
       61 agcaaccggt agagctcggg aaaacgcacg tcgtagcaaa cggagagccc gacggtcgcg
      121 gcgcccgcct tcgcggtgac aatctctgag ccggccgcga tggtccggct ctccaggtat
      181 tcgcgatccg gggccttcac gtcgaagagg tgtatcttgc ggtaaacagc cgacatcttg
      241 ccgtcgggcc cgaagaaagt ggaggtatta tgcagtttcg tggattgggg atctccttcg
      301 aggatcgaac ccccgagcag gtataccccc agctcctgcg cgagtccgcc gaggaacctc
      361 gtcgtcggcc ctggcacggg ctcggcattc tccttgtaga cttcctcgag gccgtggcag
      421 ttccacagct cgggcagggc tatcaggtcg gcccccgagg atgccgccga acgtatgagc
      481 ctttcggccg tgtcgaggtt ctcccctttg ttcggagtcg aggacatctg tatcgccgct
      541 gccgtgatct cacgcccgtt catcgcgcca ttcctttcta tgtttgcgct atatagagta
      601 ctacccgggc cattccgttg tcgcttcgac gccggatctt tgttcggcga gtacgcgcag
      661 aagcgtctcc tcgtcgtcga caacgcagtc tagcgggtca cgtaccatcg ggttcagtct
      721 ccccccgata aaggccgccc gggtgtcggt gtggagtccc accaggagct tctcgctttt
      781 ctggaggtac ttctggccgt aggctatgcc gagctccacg catgctccct cgtcggggac
      841 gcgcccgtcg agcacgaata agaacacgtc tgagtcgagg atcctggatc tatcgagctc
      901 gaacattgcg cggcgccgct cctcgggggc catcctatcg taaggcggcc tgtcgcgctc
      961 gacgccgtct cgttgcggga ggaagacctc gaagccgagg gcttcgagcc tgtgcgtaag
     1021 ttcgaggttg aagcgcctct cggccatcga gaacagtggt cctgcgaggt agaccagcac
     1081 gtctttacac gctccgagaa tcagacgggc cgtagagtct cgcctccagg acgtgatggg
     1141 catcaagttc ccgcaagcca aggtagtagc gggccgtctg cagtactgct tccagaggcg
     1201 ccaggccgac gagctcggtc gactctacgg tggcgccgaa ctcggcagcg gccgagcgaa
     1261 cggcttccag ggccacgggg atggcggtcc gttcgaggtc ggtcaggttc atcgagacct
     1321 gggccttgcc acccacaccg aagccgagcg ccctcacggc cggaaggccg ccgccgctct
     1381 ccctgacccg tctggccacc gttcgtgcga cctcgacgtc gtccgtatcc aggtacgcgt
     1441 tgaaagcaac caggaaagga cgggcgccga cggcgacggc tcccacgtgg gggtcgagtg
     1501 ttcccggacc gtaatccggc ttccacagtg ggtcttcgag gcgggatgag acgccctcgt
     1561 agccgcctcg tctaacgtcg gggagggatc tgcggtgctt tcccgtcgcg gcagacccgt
     1621 agaggaagac gggaaacccc agggaaccca ggccctcgcc gacccggtgt gccagcttga
     1681 cggcatcatc catcgtggcg ccttccaggg gcacgaaggg gagcacgtcg agcacgccca
     1741 tcctggggtg ctctccggac tgggaggcga gatcgagttc ggcggcgcac gcctctgcca
     1801 gggcgatggc gccctcgagc acggggttct cctcgccggc gaacgtgatg acgctcctgt
     1861 tgtgatcggg gtctgagtga aggccgagga tgcggacgcc cgggacgctg ctgaccgcct
     1921 ccgtgatgcg tgagatcttc gtgtcgtcct tcccctcgct gaagttgggg acggcctcga
     1981 aaagcggatc gcgcatggct gttcagcgcg ctccgagcgc gcgatccagg tgtacagcag
     2041 cgctgatgag cgccaggtgc gtaagagcct gggggaagtt gccgagggcc tcgcccgagg
     2101 acccgagttc ctcggcgtag aggcccaggt ggttggcgta agagaacatc ttctccaggg
     2161 cgaggcgcgc ttcatcgagg cgtcccgcct gggtgaggca ctccacgagc cagaaggagc
     2221 acaggctgaa gctgccctcg tccccctcga ggccgtcggg ggctgcatcc tccggccggt
     2281 aacgcttcac gagcgtgtcg tgggcgagtt cctgctggat acaatccagg gtggagagcc
     2341 aacgagggtc cgtggggccg acgaacttga ccagcggcat catgagcagc gaggcgtcga
     2401 gagcgtccga gccgtaatac tggacgaagc tctccttctc ggggttccag cctttctcca
     2461 tgacctcctc gtagatcgcg tcgcgctcgg ccatccagcg cccgtctccc gaaggcagtc
     2521 cgcgctggcg ggcgatcctg cccgcccgct ccagggccac ccagctcagg agcttggaag
     2581 agacgaactg gcgcctgcca cccctgacct cccagatacc ctcgtcgggc tcacgcca
//
LOCUS       DATMVF010000262         5690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_583841, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000262 DATMVF010000000
VERSION     DATMVF010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583841"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VN178_13390"
     CDS             complement(<1..702)
                     /locus_tag="VN178_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWS82006.1"
                     /translation="MKLTFEVWPNMPWGRLEAAGPAWNSWGDRPLVWCAERIAECGYD
                     GIDVIFGKIQEIPEDEYPAFAEEFPARLRELGLEFGYIGAHSTFVSPRHFDRERGIAG
                     FKKAVDAAADLGASSVCTLLGDGYYDPPLNILLSRKDAWAQAVGAVGEVATYAADKGV
                     NVSIELLQGSIVNRVPLLLRLIDEVDRENVRATVDTGTFYTTVKPFMDVKAAIRELGD
                     LIDIVHVKDEIGLPSI"
     gene            complement(699..1478)
                     /locus_tag="VN178_13395"
     CDS             complement(699..1478)
                     /locus_tag="VN178_13395"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HWS82007.1"
                     /translation="MKVLYAGDSAIMAVLGLEGAEVYPLVDQVWDAGTHLQQALEESG
                     IEVTRMLSHVAYSDFPETAESLAEYDALILSDIGHDTLVTYPGERRMHVPMGPNRLKE
                     IVRYVASGGGLIYCGGYFTYQGHYGKGRWYGTPVAEILPLETLPLPDDRVEAPEGAPV
                     FDIDSGHPVTSGIDWSDPPIFMGYNRTGGTQNGGRLLGRIGGGDPLLAVGEHGSGRVL
                     ATVADPAPHWGSDFVRWSHYGRFWNQAVRWVSRADKGENYG"
     gene            complement(1475..2437)
                     /locus_tag="VN178_13400"
     CDS             complement(1475..2437)
                     /locus_tag="VN178_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWS82008.1"
                     /translation="MGVDLGGTNIRSAVFDDGMQILGRSERQTRAQEDADDVIRRMAT
                     CVREALEQASVAAGEVAGVGVGAAGLTDWRTGVVVLASNLGWRNVPVRERLEQELDGL
                     RVEVDKDTNVAALAEARLGAGREFEHFLYVTAGTGIGGGLILDGALYRGATGGAGDVG
                     HIVVDPEGPRCGCGDYGCVEVFSSGAGMVDRARETLAKGGDDGESSALDPESLTTEDI
                     FEAARRGDAVAIRIVERAGAALGLALADYVNINNPEAIVLGGGVTRAGETYLGPVERE
                     LKRRALAALGEIVELVPPGLGDDVGVTGAALLLRESASQEEGGA"
     gene            complement(2470..3207)
                     /locus_tag="VN178_13405"
     CDS             complement(2470..3207)
                     /locus_tag="VN178_13405"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWS82009.1"
                     /translation="MSGAGSYLGAVGRMVQRLQSEQMGSIQEAGRLVAEVLRADRLVH
                     VFGTGHSHMLAEEGLYRAGGLAPVNAILESGLMLHEGAAASTRLEKLPGYSSIVADKY
                     GFLEGDLLIVVSNSGVNAVPVEMAMLAKEAGLKVLAVCSMAYSKAAELKAGVPARLYE
                     IADLTLDNLGTPGDAEVEVDETGLKVGPTSTVIGAALLNAVFVEAACALAAGGTEPPV
                     YRSGNMPGATDHNRWLIERFGGRIRHL"
     gene            complement(3204..4025)
                     /locus_tag="VN178_13410"
     CDS             complement(3204..4025)
                     /locus_tag="VN178_13410"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWS82010.1"
                     /translation="MVVDHTGLYRYRLEVEMRSFDQGGVQKNSSVPIYSQVERIVMDM
                     IDSGRLSPGQRAPSEREIADTLGISRMTARAAMSSLVADGYLYSVPGKGTFVSNPKMR
                     QDLLELTSFTQDMRKRGLKPGAKLLEAGVTDGAPEKIYRALGLSGADGLLRIHRLRTA
                     DEEPMCLETSYLPKDYFTWLLEEDLESGSLYRALESHGVELVRAEEHLEATLVRETES
                     ELLTVPVGYPALLIERTAYTEEDKAIEFVKSLYRGDRYRFSAKLFKRRPRRGGLP"
     gene            complement(4914..5360)
                     /locus_tag="VN178_13415"
     CDS             complement(4914..5360)
                     /locus_tag="VN178_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82011.1"
                     /translation="MESVSLGKSQEDGRAETQTTYRFGPPPASEAESVDDSSVPHFKN
                     GREPDKRERAREVLRRVGGARTAALPAQVLSEFEGACSRKLEPRPDSEALRREVERLM
                     LAFPVLGLTAPVVLEVLRGVGEHMLSYYEAQRYGRSPASGRWPDPE"
BASE COUNT         1025 a         1957 c         1613 g         1095 t
ORIGIN      
        1 gatggagggc agcccgatct cgtcctttac atgcacgatg tctatgaggt cgccgagctc
       61 cctgatggcc gctttgacgt ccataaaggg cttcacggtg gtgtagaagg tcccggtgtc
      121 caccgttgcc cggacgttct cccggtccac ctcatcgatg agccgcagca ggagcggaac
      181 tcggttcacg atgcttccct gcaacaactc gatgctcacg ttcacgccct tgtcggctgc
      241 gtaggtcgcg acctcgccca ccgctcccac ggcctgcgcc caggcatcct tgcggctcag
      301 gaggatgttc agcggcgggt cgtagtagcc gtcaccgagg agggtgcaga ccgagctcgc
      361 cccgaggtcc gcggccgcgt caacggcctt cttgaacccc gctatcccgc gctcgcggtc
      421 gaagtggcgc ggcgagacga aggtcgagtg ggctccgatg tagccgaatt cgagtcccag
      481 ctcgcgcagc ctggcgggga actcctcggc gaaggccggg tactcgtctt cggggatctc
      541 ctggatcttt ccgaagatca cgtctatgcc gtcgtatccg cactcggcta tcctttccgc
      601 gcaccagaca agcggtctgt ccccccaaga gttccaggcg ggaccggcgg cctcaagccg
      661 gccccacggc atgtttggcc aaacctcgaa ggtaagcttc atccgtagtt ctcccccttg
      721 tccgctctcg aaacccagcg gaccgcctga ttccagaacc gtccgtagtg cgaccagcgc
      781 acgaaatcgc tcccccagtg aggcgcggga tccgccaccg tggccagcac gcgcccggat
      841 ccatgctccc caacggccaa aagcggatct cctcctccaa tgcgtccgag cagacgtcca
      901 ccgttctggg tacccccggt gcggttgtat cccataaaga tcggcggatc gctccaatct
      961 attccggaag tcaccggatg tcccgagtcg atatcgaaaa cgggagcccc ttcgggcgct
     1021 tcgacacggt cgtcgggtag tggcaacgtc tcaaggggca ggatctcggc caccggggtc
     1081 ccataccacc gcccctttcc gtagtggcct tgataggtga agtacccccc gcagtagatc
     1141 aaacccccgc cagacgccac ataccggacg atctccttca gccggttcgg gcccataggc
     1201 acatgcatac ggcgctcccc aggataggtc accagggtat cgtggccgat gtcgcttagg
     1261 atcagcgcat cgtactcggc aaggctctcg gcagtctcgg gaaaatccga gtaggcaacg
     1321 tgcgagagca tgcgcgttac ctcgatcccg gattcctcga gagcctgctg caaatgggtt
     1381 ccggcgtccc acacctgatc gacgagaggg tagacctcgg ctccttctag acctagcacc
     1441 gccataatgg cggaatctcc cgcgtacagt actttcatgc tcctccctct tcttggctag
     1501 ctgactctct gagcagcagg gccgcgcctg tcactccgac atcgtccccc aagccgggcg
     1561 gcaccagctc tacgatctcg ccaagagccg ccagcgcccg ccgcttcaac tcgcgttcga
     1621 cgggacctaa gtacgtttcc ccagctcgcg ttactccccc gcccaggact atggcctccg
     1681 gattgtttat gttgacgtag tcggccagcg ccaaccccag cgccgcgccg gccctctcca
     1741 caattcggat agccaccgca tctcctctcc gggcggcttc gaatatgtct tctgtggtaa
     1801 gactctccgg gtccagggca gaagattcgc catcgtctcc acctttggct aaggtctccc
     1861 tggcccggtc gaccatgccg gcgcctgagg agaatacttc cacacagccg tagtcgccgc
     1921 agccgcaccg gggtccctcc ggatccacca caatatgtcc gacgtcaccg gcgccacccg
     1981 tcgcgccccg gtaaagagcg ccgtccagga tgagcccacc accgataccg gtgccagccg
     2041 tcacgtagag gaagtgctcg aactcgcgcc cagcacccag tcgagcttcg gccaaggcgg
     2101 ccacgttggt atccttgtcc acctcgacgc gcagcccatc tagttcctgc tctaggcgct
     2161 ccctgacggg aacgttcctc caccccaggt ttgacgccag gactaccacc ccagtcctcc
     2221 agtccgtaag tccggcggca ccgaccccga ccccggcgac ctcgcccgcc gcgacggaag
     2281 cctgctccag cgcctcgcgc acgcaggtcg ccattctgcg gatcacgtca tccgcgtcct
     2341 cctgtgcccg tgtttgcctc tcggatcggc ccaggatctg catcccgtcg tcgaagacgg
     2401 cacttctgat gttcgtgcca cccaggtcga cgcccacgga ggcaatccga gcgctgtctc
     2461 ggtcgccgct cacaggtgtc gaatcctccc cccaaaacgc tcgatgagcc accggttgtg
     2521 atcggtagca cccggcatgt tgccgctgcg atagacgggg ggctccgtcc ccccggccgc
     2581 gagcgcgcat gccgcctcca cgaagaccgc gttgagcaac gcggccccga ttacagtcga
     2641 tgtaggcccc accttcaggc cggtctcgtc gacctcaacc tccgcatccc ccggcgtgcc
     2701 caggttgtcc agggtgagat cggcgatctc gtacaggcgc gcgggcaccc cagctttcag
     2761 ctccgccgcc ttcgaatatg ccatggagca aacggcgagc accttgagcc cggcttcctt
     2821 ggccaacatg gccatctcca caggcacggc gttgaccccg gagtttgaga ctactatcag
     2881 gaggtctccc tctaagaagc cgtatttgtc ggctacgatt gacgaatacc ccggaagctt
     2941 ctccagcctc gtcgatgcgg ccgcgccctc atgcagcatc aggccggatt ccaggatcgc
     3001 gttgaccgga gctaggcccc ccgcccggta gagcccttcc tctgcgagca tgtgcgagtg
     3061 gccggtgccg aacacgtgga ccagccggtc cgcgcgcaga acttcggcca ccaaccttcc
     3121 cgcttcctgg atcgatccca tctgctcgga ttggaggcgc tggaccatcc tgcccacagc
     3181 tccgaggtag cttcctgcac cgctcatggg agcccccccc tcctgggcct acgtttgaac
     3241 agcttcgcgc tgaaccggta gcggtctccc cggtagagag atttcacgaa ctcgatcgcc
     3301 ttgtcctcct ccgtgtacgc ggtccgctcg atgagaagcg ccgggtaccc gaccggtacg
     3361 gtgagaagtt cgctctccgt ctcccgcacc aaggtcgctt ccaagtgctc ctcggccctg
     3421 acaagctcga cgccgtggct ctcgagcgct ctgtacaggg accccgattc gagatcttcc
     3481 tcaagcagcc aggtaaagta gtccttgggc aggtagctcg tctccagaca catgggctcc
     3541 tcgtcggcgg tccgaagtct gtggatgcgg agcaggccat cagccccgga aagtcctaga
     3601 gcccggtaga tcttctccgg agctccatcg gtgacgcccg cctccaagag cttggcgcca
     3661 ggcttgagcc cgcgtttacg catgtcctgc gtgaagctgg ttagctccag taagtcctgt
     3721 cgcatcttgg ggttggagac gaaggtgccc ttccccggca cgctgtagag gtagccatcg
     3781 gcgacgaggc tggacatggc cgccctcgcc gtcatgcggc tgatgcccag ggtatcagcg
     3841 atctccctct ctgagggcgc gcgctggcca ggcgagagtc taccgctgtc tatcatgtcc
     3901 ataacgatcc gctcgacctg agagtaaata ggaactgagc tgtttttctg gacaccaccc
     3961 tggtcaaagg atctcatctc cacctccagt ctataccggt atagacctgt atggtccact
     4021 accatagcaa gattcacttt tagtgtcaaa cgctgtaatt atggttggca ccccattcgc
     4081 atccgagcgg gtactctagc gaccagggta ctcacttctc gccgctggcc gattccggca
     4141 gggaatcaga ctgcaagtgc tatagatcgc ccgctgtgcc gaacgggttg cagcacggtt
     4201 gcagtatggc gagacccgcc tgatctccac acctcacgtt ttacctgcaa attggaagaa
     4261 aataatagag ccgaagagcg gactcgaacc gctgacctca tctcattacg agtgcgctgc
     4321 tcgccatctg ggcgcaaccg gagggcctgc tagctgaatg gcggccttgt accccctaac
     4381 gcaaacacga aagcctcact gaatgggggg ctggggtatc gctcctactc accgatatat
     4441 tgcgacccac tatttcttag gcacgcataa cttccagctt ctgaatctca gcctgggtca
     4501 gtaaggcgcg aaacctgacc tccgtattta tgcagtcgtg cattgatgcg agcgcatccg
     4561 tgccattgtg caaacgtgcc cctgaggctc tctactctct agccaattcc agccgctcgg
     4621 aacccttagt agacagctca gcgcgtaacg ccagttcggt gcggtttgcc gggaatcaga
     4681 ggtgcaccac agcgcaagta ccatcacttc ctgtggacga agatcgcgag ctcgccggca
     4741 cctgataagg gatcgtgtcc gttatcccgt catccccgtt ctttctccgt ctggttccat
     4801 acctctgggt ggatccttct atgagcttct aacccaggac agcaaggtcg aatgcggggt
     4861 caagcgggtt gatgaaagac accccgtcga gtaccgcctc agggccgaaa tcctcactca
     4921 ggatcaggcc acctgcccga ggcgggcgac cgcccatatc tctgggcctc gtagtaggag
     4981 agcatgtgct ccccgacgcc ccgcaggact tcgagcacca cgggggcggt gagccccaag
     5041 acagggaagg cgagcatgag ccgctcgacc tcccgccgta gcgcttctga atccggcctg
     5101 ggctcgagct ttctcgagca cgcgccctcg aattcggaga ggacctgcgc cgggagggcc
     5161 gcggttcggg cgccaccgac gcgacggagc acctccctgg cacgctcacg tttgtccggc
     5221 tccctgccgt tcttgaagtg aggcacagac gagtcgtcga cggactctgc ttctgaggcc
     5281 ggcggcggac cgaaacggta ggttgtctgg gtctcggccc ggccgtcctc ctggctcttg
     5341 ccgagcgaga ccgactccac gcgcagcccg acgagctgct tgcgcttccc gaacgcctca
     5401 ggccgatcct cctcgatctc gaccaggcgt cgtgtgagcg agagtagtca gccgtgtgtt
     5461 tgccgccccc gaacgcccgg tgcaggcctg cccgcgtcct ctctcgcatg gtctcgcgct
     5521 cgcactccgc gaacgacgca agcatctgga agatgagctt gccgctgggg ttggaggtgt
     5581 ctatgggctc ggtggcgctc tttagggaca cgccgaaggc cagtagccta ttgtggacgc
     5641 cgacaatgac caggagtgac ctacctagcc gatccaggcg gtagacgagg
//
LOCUS       DATMVF010000263         9212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_678639, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000263 DATMVF010000000
VERSION     DATMVF010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678639"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VN178_13420"
     CDS             <1..765
                     /locus_tag="VN178_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS82012.1"
                     /translation="EIGDLAITINDMLSHLEITLARLEETLDRQRRFVADASHELRTP
                     LTSIHSYAQMLEEWALRDPQIGPESVAAIQRESERMKGLVENLLELARGDEGMKLNLK
                     EDDLTEVVEEAAESARASVNGKVLIEHPTPQQKTRRVSAVFDRERLRQALSILLDNAV
                     KYSPEGGRVTIKIAEEDGSVGVEVADTGIGIPEDQIPHVFERFYRAEEARSTEGLGLG
                     LSIARQIAEDHKGSIEVRSKPGEGSTFIIRIPRSEA"
     gene            1040..1687
                     /locus_tag="VN178_13425"
     CDS             1040..1687
                     /locus_tag="VN178_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWS82013.1"
                     /translation="MPQIPKEAFRRTDETPDEEFYRTPRLVTHIDEGAIAAVTQLYRE
                     HFPAGGEILDLMSSWVSHLPPEVAYRRVVGLGMNEAELRRNERLDSYIVQNLNTNPGL
                     PFEEGEFDGVGICVSIDYLTRPVEVLREVGRVLKVGAPLITTFSDRCFPTKAVEIWRR
                     LDDRGHMRLVEEYLQEAGNFRNVRSLDRSPRRLFSDPLYAVVGESTGPYSGGLSS"
     gene            1720..1905
                     /locus_tag="VN178_13430"
     CDS             1720..1905
                     /locus_tag="VN178_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82014.1"
                     /translation="MNIRQANGIGRVHVAKPQFRETYCGRPINDEDWVTTTREANCTG
                     CARAGAPGLERTPSASR"
     gene            2032..3027
                     /locus_tag="VN178_13435"
     CDS             2032..3027
                     /locus_tag="VN178_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWS82015.1"
                     /translation="MKIPGTRLIKKAESKGNEVEVPVAENLGLVDFFKLTYKEMDEDH
                     VMAFAGNLTYKGLFAIFPFFTFLLSLLGLFNATDLVNTMVDKLSGVAPQSATEFIEGQ
                     LLSITQSQAESAFTFGAIVSILLALWGVSGAFRSVMEAMNVMYEVEEDRPFWKVYGLS
                     IFMSLAVVVLLVGALGLVIFGESIGVRVADLVGLGSVFATLWSIVQWPVVALVVLFAF
                     ALIYYFAPAAKQRFRWISPGSIVAFVFWLAFSLLFSLYASTAGSYNETYGSLAGVIIL
                     MLYVYYSALIMLVGAEINQVIEWHIPGGKNEGEKIPEDDRRPDAHRLNRESGETR"
     gene            3049..3876
                     /locus_tag="VN178_13440"
     CDS             3049..3876
                     /locus_tag="VN178_13440"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HWS82016.1"
                     /translation="MRIYGLDFTSAPGRRKPLIVLGCTLEGDSLRVEDSETLTDFSGF
                     EDFLQRPGPWVCGMDFPFGQPRSLVAALGWPESWEGYVGEVGKLPKEEFEGKIRADMA
                     TRPAGKKWRYRLADRRSGSSSAMMLFRVPVGKMFYQGAPRLLASGVRVEPCRRNGDER
                     VAVEAYPAVVARRFLGRTAYKRDAAPDTPERRSARETLLSGLESAVLRDIYGFAVETN
                     DHWREEFLRDPSADALDSLLCAVQAAWAYEKRDDNYGVPAECDADEGWILDPALLDE"
     gene            complement(4017..4712)
                     /locus_tag="VN178_13445"
     CDS             complement(4017..4712)
                     /locus_tag="VN178_13445"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HWS82017.1"
                     /translation="MAPLLLVLFIALFVVVFLFLLPILFSLQAVGSLFVYPKQLRAIF
                     GNKILRRNHALEHATIVVMMEREPGRRLNGFSTDDGFFVQGVRSISEVESAAREALRR
                     LQSGEKRLAIHRNCGTTIVAANLLAAIFFLITLGLVLYLGVGNLYLMILGSVVLALAL
                     RIPLSLLLQRFVTTDADLTNAEVGWVEPAQPGDLKSGIFGLLLAASTVRVRVFHTDPE
                     AVEIFRDDGAIVR"
     gene            complement(5015..5410)
                     /locus_tag="VN178_13450"
     CDS             complement(5015..5410)
                     /locus_tag="VN178_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HWS82018.1"
                     /translation="MQPFVKPKKPLQRSRLALVTTGGVHLPDQERFDIDDPAGDCSYR
                     EIPVTANQLTWTHAYYRPDEGTDLDAVFPLETLRRLVSDGAVGGLAARHFSFMGAIHD
                     PAPLVERTAPEVADKLVEDQVDVVLLTPS"
     gene            complement(5422..6504)
                     /gene="ychF"
                     /locus_tag="VN178_13455"
     CDS             complement(5422..6504)
                     /gene="ychF"
                     /locus_tag="VN178_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWS82019.1"
                     /translation="MKVGIVGLPNVGKSTVFNSLTRAGAEAQNYPFTTIDPNLGVAAV
                     PDERLVAVAGVIGSKGVVPATVDFVDIAGLVRGASVGEGLGNQFLGHIRECDAIAHVV
                     RCFEDENVVHVHAGVDPRGDIETIDTELLLADLASVERRLERVGRAAKSGDSRLQAEA
                     AELEQLRDHLSDGSPARVFPATETLAEAMSTLITAKPTLYVANVDEDSVVQGSRYSSQ
                     VEKHASSEGAEAVRICAKLEAEVAELPEDEAAEYLAMLGVETTGFEEFVRAAYRLLGL
                     VTFFTAKEKEARAWTVREGSTVRQAAGRIHTDMERGFIGAEVGDWQDIVGAGSWARAK
                     EEAKVRLEGRDYVVRDGDTVLVRFNV"
     gene            6608..7084
                     /locus_tag="VN178_13460"
     CDS             6608..7084
                     /locus_tag="VN178_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWS82020.1"
                     /translation="MDRQLQIQILLDHYEKPRHRGALEDHDVAMPGGNPGCGDVVTVY
                     LKAADDQEHIEEATYEGEGCTISMAASSMLLEDVHRGGLTMDQVLEMDYHEMIEQLGR
                     QVVASRPRCATLGLGTLKAAIRKYQKDRRLESAGVTRPDDGGFVFGEHAAEAAREH"
     gene            complement(7110..7544)
                     /locus_tag="VN178_13465"
     CDS             complement(7110..7544)
                     /locus_tag="VN178_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82021.1"
                     /translation="MEEREERGGAVRVGMIWGGIGGVVGFLVSLLGSLVGILVAGFIG
                     FSCGRRAAVAGNRAGALSGLVGGAVAAPVYVLGSALGALLAARQIGAAGIAATLSDML
                     GMQVSADEAWIYFLFSLILSAILEAVVLILVSSAAGAWAKRG"
     gene            complement(7596..8015)
                     /locus_tag="VN178_13470"
     CDS             complement(7596..8015)
                     /locus_tag="VN178_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82022.1"
                     /translation="MIPRLLPEQRGMALGLAIIVVVIVGVMGAGLMTIVATDLEAIVA
                     VNRGEQAFELAEAGIEVAKAHLANDPSPSDWSSGELHMAGMGEGSVTVTVEYDDESRT
                     FEAISTGEYRETSRKIEAIFSIEEAEPKLLSWRELYE"
     gene            complement(8012..8647)
                     /locus_tag="VN178_13475"
     CDS             complement(8012..8647)
                     /locus_tag="VN178_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82023.1"
                     /translation="MVNPARRYSWKVETGFTLVEVLVSAMMMFGVLFALYAIFDVSVR
                     AFGYGGDRLEAIGNARLALGRMEREIRAAYPYDLTSDPPRRYLFLDPTDPTRPGVPSA
                     TRIAFGNETGGDRSIEGSEVIGYYLSGHDLKRSKGGSAQTLVDSIPADGLQFTPCRSP
                     DDCPPTLAITNEAEIKLLRIELAVEVRRRGQDTARQTLATDVYLRNREDQP"
     gene            complement(8641..9030)
                     /locus_tag="VN178_13480"
     CDS             complement(8641..9030)
                     /locus_tag="VN178_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWS82024.1"
                     /translation="MISPTKSAAGYSLVEVMVAMVVLTVAVVPMVGMFDAAIGAADAS
                     ADYDKARTCAVQKLEHLKSLPYEAVEGGLQGGVCDPSGFGYTIATESIGTDLDDGSGE
                     EGLTMVTVTVDWDGEDSYVVSGVVSRW"
     gene            complement(9014..>9212)
                     /locus_tag="VN178_13485"
     CDS             complement(9014..>9212)
                     /locus_tag="VN178_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82025.1"
                     /translation="PSSCGSDAAQNGATRTLEFNSNGTVRTLRPGQSGTVVISSEDGI
                     PTCPVVFNAPTSRIRIGDISY"
BASE COUNT         1781 a         2806 c         2947 g         1678 t
ORIGIN      
        1 gagataggag atctcgccat cacgatcaac gacatgctct ctcacctcga aattaccctg
       61 gcccgcctcg aagagacgct tgaccggcag cgccgcttcg tcgccgacgc cagccacgaa
      121 ttgcgtactc ctctcacctc catacacagc tacgcgcaga tgctcgaaga gtgggcgttg
      181 agagacccac agatcggacc ggagagcgtg gcggcgatac agcgagagtc ggagcgcatg
      241 aagggtctgg tagagaacct gctcgagctc gctcgcggtg acgaagggat gaaactgaac
      301 ctgaaagaag acgacctcac cgaagtagta gaagaagccg cggagtcggc gcgggcatcc
      361 gtgaacggca aggtgcttat cgagcatccg acccctcagc agaagacacg gcgggtaagt
      421 gcggtcttcg acagagagcg tctccggcag gcgctctcga tcctcctgga caacgccgtg
      481 aagtactcgc cagaaggagg gcgggtcacc atcaagattg cggaagagga tggtagcgta
      541 ggggtagagg tcgccgacac cgggatcggg atacccgagg accagatccc acacgtcttc
      601 gagcgcttct accgcgcgga ggaagcccgg tcgaccgagg gcttggggtt ggggctgtcg
      661 atagcccggc agatcgccga ggatcacaag ggtagcatag aggtgcggag caagcccggg
      721 gaaggatcga ccttcatcat acgcatacca cgctccgagg cttgagttct cgtcccttcg
      781 ccacgattac ttccgggaac gcagcgtcgt cgccggcggg gtctcgcgat gctcgggcct
      841 ggcctacgag ctatgcgtaa tcccgtagat cgctgccggt tggaggagcg caacgtctgg
      901 tgcggtggaa tccggcatgc gcgggaatga cggttttgtc gaaagcgggc gggggtagga
      961 gtctggctag cccaggaagc cgtacgctgc ccaacccatc atgcgaattc cttatgaggg
     1021 gtcagaagga ggcaaggatt tgccgcagat tccaaaggag gctttcagac gtaccgacga
     1081 gacgccagac gaggaatttt ataggacgcc ccggcttgtc acccacatag atgaaggcgc
     1141 gatcgccgcc gtcacgcaac tctacaggga gcacttcccg gcgggcggag agattctgga
     1201 cctcatgagc agctgggtta gccatctacc gccggaggtc gcgtaccggc gcgtggtcgg
     1261 gctcggtatg aacgaggcgg aattacgccg caacgagagg ctcgactcct acatcgtgca
     1321 gaacctgaac acgaatcctg gactgccttt cgaagaaggg gagttcgacg gggttgggat
     1381 ctgcgtctcc atcgactacc tgacaaggcc cgtagaggta ctacgcgaag tcggtcgggt
     1441 gctgaaggtc ggggcaccac tgatcacgac cttctcagat cgctgttttc ccacaaaggc
     1501 ggtcgagatc tggcgccgac tggatgaccg tgggcacatg cgcctcgtag aagagtatct
     1561 ccaggaagcc ggcaacttcc gaaacgtccg cagcctcgac cggagtccgc gccgcctctt
     1621 ctcggacccg ctctacgcag tcgtaggtga gagtacagga ccttacagcg gcgggctctc
     1681 aagctaaact ggacgcaaag gaaccggacg aggagagaga tgaatattcg ccaggcgaac
     1741 gggatcggca gggtgcacgt ggccaagcca caattcagag agacgtactg cggcaggccg
     1801 atcaacgacg aggactgggt aaccaccacc agggaagcca actgcaccgg ttgcgcccgg
     1861 gccggggcac cgggactgga aagaactcca agcgcgtcgc gctgatctgc ggatagggga
     1921 gagtttcggc cgcacgcttt acgccggcac cacatgttag aagtatggcc tcccgcgggt
     1981 aaactgagtg acgaatcaat ctgaggcttt cacctgcggg aggacgaacg catgaagata
     2041 ccgggtacgc ggctgatcaa gaaggccgag agcaaaggaa acgaggtcga ggttcccgtc
     2101 gcggagaacc tgggtctcgt cgacttcttc aagctgactt acaaggagat ggacgaagac
     2161 cacgtcatgg cgttcgccgg caacctcacc tacaagggcc tcttcgccat cttcccattt
     2221 ttcaccttct tgctctcgct gctgggcctc ttcaacgcga ccgacctcgt caacaccatg
     2281 gtcgacaagc tctccggcgt agcgccgcag tcggcaacgg agttcatcga aggtcagcta
     2341 ctcagcatca cgcagagcca ggccgagagc gccttcactt tcggggcgat cgtctcgatc
     2401 ctgctcgcgc tctggggtgt ttccggggcg ttccgctcgg ttatggaggc catgaacgtc
     2461 atgtacgagg tcgaggaaga cagacctttc tggaaggtgt acgggctctc gatcttcatg
     2521 tcgctcgcgg tggtcgttct cctggtcggc gcgctcgggc tggtaatctt cggcgagtct
     2581 atcggcgtga gggtggccga cctcgtcggc ctggggtccg tcttcgcgac gctctggagc
     2641 atcgtgcagt ggcccgtcgt ggccctggta gtcctcttcg ccttcgccct gatctactac
     2701 ttcgcccccg ccgccaaaca gaggttccgt tggatcagcc cgggatcgat cgtcgccttc
     2761 gtgttctggc tcgccttctc cctgttgttc tccctgtatg cgagcaccgc cggctcctac
     2821 aacgagacgt acgggtcgct tgcgggtgtg atcatcctga tgctctacgt ctactactcg
     2881 gctctcatca tgctggtggg ggccgagata aaccaggtga tcgaatggca catccccggc
     2941 ggcaagaacg agggagagaa gatccctgaa gacgaccgca gaccggatgc gcatcggctg
     3001 aacagggaaa gcggcgagac ccggtaggtt cgccttgaac ggtcccgcgt gaggatctac
     3061 ggcctcgact tcacgagcgc ccctggccgt cggaaacccc tcatcgtcct cggctgcacg
     3121 ctggaaggag actccttgcg cgtcgaggac tcggagacgt tgaccgactt cagcggcttc
     3181 gaggactttt tgcagagacc gggtccctgg gtgtgcggga tggacttccc gttcggccaa
     3241 ccaagatccc tggtagccgc cctcgggtgg ccggagagtt gggaggggta cgtcggcgag
     3301 gtcggcaaac tcccgaaaga ggagttcgag gggaagatcc gggccgacat ggccacaagg
     3361 ccagcgggca agaagtggcg ttaccggctc gccgaccgca ggagtggatc cagcagcgcg
     3421 atgatgctct tcagggtccc cgtaggcaaa atgttctacc agggcgcgcc acgcctcctc
     3481 gcctctggcg tccgtgtcga gccgtgcaga cggaatgggg acgaacgcgt ggccgtcgaa
     3541 gcctacccgg ccgtcgtcgc ccgccgcttt ctgggacgca cggcatacaa aagggacgca
     3601 gcacccgaca cgccagaaag gagatccgcc agggagacgc tgctctccgg cctggagtca
     3661 gcagttctca gggatatcta tggattcgcg gtggagacga atgaccattg gagggaagag
     3721 ttcttacggg acccttccgc cgacgccctc gactcgctcc tgtgcgcggt ccaggcggcg
     3781 tgggcctacg aaaagagaga cgacaactac ggcgtaccgg cggagtgcga tgccgacgaa
     3841 ggttggatcc tggacccggc gttgctggac gaatagagca gtttgctgcg caaactgcgg
     3901 ctacaggtta caggcttcag gtatggggct gagccatgac ccggcagggg aagcgtacac
     3961 atcaagtgac taactcttgc agttcctgat gcctgtagcc tgaaacgtga agctttctac
     4021 ctaactatcg cgccgtcgtc gcggaagatc tcgaccgctt cagggtcggt gtggaagacg
     4081 cgcacccgca cggtcgacgc ggcgagcaag agcccgaaga tgccgctctt gaggtcgccc
     4141 ggctgcgcag gctcgaccca accgacctcc gcgttcgtca ggtcagcatc ggtggtaacg
     4201 aaccgctgca ggaggaggct caaagggatg cgcagggcga gagcgaggac gacgctgccg
     4261 aggatcatga ggtagagatt acccacgcca aggtagagaa ccagtccgag ggtaatcagg
     4321 aagaagatag cggcgagcag gttcgcggcc acgatggtcg taccgcagtt gcggtggatg
     4381 gccagcctct tctccccgct ttgcaacctg cgcagggcct ctctggcggc gctctcgact
     4441 tcggagatag agcgtacgcc ctgaacgaag aacccgtcgt cggtggagaa cccgttcagc
     4501 ctgcgtcctg gctccctctc catcatcacc acgatggtcg cgtgctccag ggcgtggttg
     4561 cggcgcagga tcttgtttcc gaagatcgcc cgtagctgct tgggatagac gaaaaggctc
     4621 cccacggcct gcagcgagaa cagtatcggc agcaggaaga ggaacacaac cacgaataac
     4681 gctatgaaca acaccaacaa taacggggcc acggtctcac ctttgcgatc ggtctgtata
     4741 cagctcgatt atatctcacg tttcaggtga aacctgaagc ctacaactcc acttctccag
     4801 gttgctccat gtcgtggagc agggcgagcg cggcgagcgc tacgcgctcg tgtccttcgc
     4861 gatcgtgggg tttgcccatc gggagattgt atggaaaggg tacgaagccg acccgtggcg
     4921 cactgatgcc gtgctccatg gccagggtta cggtggggat gccccgttct tctatcgcgt
     4981 tgcagaccag cccgacggac cggtggcaca ggggtcagga aggcgtgaga agtacgacgt
     5041 ctacctggtc ttcgaccagc ttgtcggcta cttcgggggc cgtgcgttct accaacgggg
     5101 ccgggtcgtg gatggctccc ataaaggaga agtggcgcgc cgccagcccg cccaccgcac
     5161 catcgctgac cagcctgcgg agggtctcga gggggaagac tgcgtccagg tcggtgccct
     5221 cgtccggtct gtagtaggcg tgggtccagg tgagctggtt ggcggtcacc ggtatctccc
     5281 ggtaggagca atcgcctgcg gggtcgtcga tatcgaagcg ctcctggtcg ggcaggtgaa
     5341 cgccgcccgt ggtaacgagc gcgaggcggg atctctggag gggtttcttc ggtttgacga
     5401 aaggctgcac cggctacgcc atcacacgtt gaagcggacg agtacggtat cgccgtctct
     5461 taccacgtag tcgcggcctt cgagacggac cttcgcctcc tctttggccc tggcccagga
     5521 gccggcgccg acgatgtcct gccagtctcc gacctcggcc ccgataaagc cgcgctccat
     5581 gtcggtgtgg atgcgtccgg cggcctggcg caccgtcgag ccttctctta cggtccaggc
     5641 tcgggcctcc ttctccttcg cggtaaagaa cgtgacgagc ccgagcagcc ggtaagccgc
     5701 ccgcacgaat tcctcgaaac ccgtcgtctc taccccgagc atcgccagat actccgcggc
     5761 ctcgtcttcc gggagctcgg cgacctcggc ttccagcttc gcacagatcc ttacggcctc
     5821 agcaccctcg ctgctggcgt gcttctcgac ctgcgagctg tagcgactgc cctgcacgac
     5881 ggaatcctca tccacgttcg cgacgtagag cgtcggcttg gcagttatga gggtgctcat
     5941 ggcctcggcc agcgtctccg tcgccgggaa gactcgtgcc ggactgccgt cagagagatg
     6001 gtcacgtagc tgttccaact cggccgcctc ggcctggagt ctggaatcgc cgctcttggc
     6061 ggcccgcccg acccgttcca gccgccgctc gacgctcgcg agatctgcga gcaacagctc
     6121 ggtgtcgatg gtctcgatgt cgcccctggg atccacgccg gcgtggacgt gtacgacgtt
     6181 ctcgtcctcg aaacagcgga cgacgtgcgc aatggcgtcg cactctctga tgtgcccgag
     6241 gaactggttg cccagcccct ccccgacgct cgcgcccctc accagccccg cgatgtccac
     6301 gaagtcgacc gttgcaggga cgacgccttt gctgccgatc accccggcca cggcgaccag
     6361 gcgttcgtcc ggtacggcgg cgacacccag gttcgggtcg atggtcgtga atgggtaatt
     6421 ctgggcctcg gcccccgccc tggtaaggct attgaatacc gtggacttgc cgacgttcgg
     6481 cagcccgact atacccactt tcatggtgaa agtgtatcaa actgtagcaa gtttacgtga
     6541 cggcggacga gggtgcgata gaatcgtcgt tagagaaact atggaataac aagtggaagg
     6601 ttacgtgttg gacaggcagt tacagataca gatactgctg gatcactacg agaagccgcg
     6661 gcatagggga gccctggagg atcatgacgt ggcgatgccc ggtggcaatc ctggatgtgg
     6721 agacgtggtt acggtctacc tcaaggcggc cgacgaccag gagcacatag aggaggcgac
     6781 ctacgagggc gagggctgta cgatcagcat ggcggcgagc agcatgctcc tggaggacgt
     6841 tcacaggggc ggtctgacca tggatcaggt actggagatg gactatcacg agatgatcga
     6901 acagctggga cgccaggtcg tcgcctcccg ccccaggtgc gcaaccctgg gtctggggac
     6961 cctgaaggcc gcgatcagaa agtatcagaa ggaccgtcgc ctggagagcg ccggcgtgac
     7021 gcgccccgac gacggggggt tcgttttcgg ggagcacgcg gcggaggcgg ccagagagca
     7081 ttgagctgcg gggcgccggg aagccctttc taaccccttt tcgcccaggc cccggcggca
     7141 gatgagacca ggatcaggac aacggcctcg aggatggcag agagaatgag gctgaaaaga
     7201 aagtagatcc aggcctcatc ggccgagacc tgcataccga gcatatccga gagggtcgcg
     7261 gcgatcccgg cggccccgat ctggcgcgca gccagtagcg cccctagcgc ggacccgagc
     7321 acgtacacgg gcgctgcgac ggccccgccg accagcccgg agagagcgcc ggcccgattt
     7381 cccgcaacgg cggcccgtct cccgcaggag aaaccgataa agcccgcgac caggatcccg
     7441 accagagaac cgagcagcga gaccaggaag ccgaccactc caccgatgcc gccccagatc
     7501 atccctaccc tgacggcccc accacgttct tctctctcct ccatgacaac attctagcgg
     7561 catgggcgac cagcggctgc gaaggcctga cacctttact cgtagagttc tcgccaggac
     7621 aggagctttg gctcggcttc ttcgatagag aagatggctt cgatcttgcg gctcgtctcc
     7681 cggtattcgc ctgtggagat cgcctcgaag gtcctgcttt cgtcgtcgta ttcgacggtg
     7741 acggtgacgg agccctcacc catgcctgcc atatggagtt cgcccgagga ccagtcggaa
     7801 ggactcgggt cgttcgcgag gtgtgctttg gcgacctcga tccctgcttc ggccagctcg
     7861 aaggcctgct ctcccctgtt gaccgccacg atggcctcca ggtccgtggc gacgatggtc
     7921 ataaggccgg ctcccatcac gccgacgatc actacgacga tgatggcgag tcctaatgcc
     7981 ataccccttt gctcgggcag gagacgcggg atcatggttg atcttcgcgg tttctgaggt
     8041 agacgtcggt cgcgagcgtc tgcctggctg tgtcttgacc ccgacgcctg acttcgacgg
     8101 ccaactctat gcgcaggagc ttgatctccg cctcgttcgt gatagcgagg gtcggaggac
     8161 agtcgtcggg gctcctgcac ggggtaaact gcagtccgtc cgcggggatc gaatcaacga
     8221 gcgtctgggc gctgccgccc ttggaacgtt tcagatcgtg gccgcttagg tagtagccaa
     8281 tgacttcgga gccctcgatg ctgcggtctc ccccggtctc gttgccgaag gcgatgcggg
     8341 ttgccgaagg tacgccgggc cttgtgggat ccgtggggtc caggaacagg tatctcctgg
     8401 gtgggtccga ggtcaggtcg taagggtagg cggccctgat ctcacgttcc atcctgccga
     8461 gggcgagcct ggcgttcccg atggcctcca gcctgtcacc gccgtaaccg aaggccctca
     8521 cgctcacgtc gaagatcgca tacagggcga acagcacgcc gaacatcatc atcgcggaga
     8581 cgagtacctc cacgagcgtg aagcccgttt ctaccttcca cgagtatctc cttgccgggt
     8641 tcaccatctg gacacgactc ctgaaacgac gtaggagtcc tcgccgtccc agtcgaccgt
     8701 aacggtgacc atcgtcaatc cctcttctcc ggaaccgtcg tcgaggtcgg taccgatgga
     8761 ctcggtggcg atcgtgtatc cgaaaccgga agggtcgcag acgccgcctt gcaggcctcc
     8821 ctcgacggcc tcgtacggaa ggctcttgag gtgttccagc ttttgtacgg cgcacgtccg
     8881 cgctttgtcg tagtcggcgc tcgcgtcggc ggccccgatc gcggcgtcga acatgcccac
     8941 catgggtacg acggcgacgg tgaggactac catcgccacc atcacctcta cgagtgagta
     9001 acccgcggcg ctcttagtag gagatatcac cgatccttat cctcgaggtc ggggcgttga
     9061 agacgaccgg gcacgtcggg ataccgtctt cagagctgat cacgacggta ccgctctggc
     9121 cgggtctgag agtcctgacc gtgccgttgg agttgaactc gagggtgcgt gtggcgccgt
     9181 tctgtgcggc gtcggacccg cacgagcttg gc
//
LOCUS       DATMVF010000264        17291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_28936, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000264 DATMVF010000000
VERSION     DATMVF010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 17291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17291
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28936"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VN178_13490"
     CDS             complement(<1..266)
                     /locus_tag="VN178_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWS82026.1"
                     /translation="MAIHAERATGKAASEAIRDVKRTTCCVVGGGPGGAVLALLLARS
                     GVDVTLLEAHPDFDREFRGDTLHPSVMEIMDQLGLADRLLELRHS"
     gene            complement(277..882)
                     /locus_tag="VN178_13495"
     CDS             complement(277..882)
                     /locus_tag="VN178_13495"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS82027.1"
                     /translation="MAEGSDRRAQILEAAFEEFSEKGFEGATIKSIARTASLQSPALI
                     YWYFPDKEALFREVLGSRIPVLRAVRDPARLLDLPPEVVLPNIARRYLSTFDDSTTQR
                     MVRLLIGEAMRRPEVSEVFGNAVIKKVLGFLKSYMARQVELGRLRPHDVRSSARAFIG
                     MLLPQAGGKLFFPVIREDGLTDEAHIQTAVNVFLKGLEPEV"
     gene            1013..1243
                     /locus_tag="VN178_13500"
     CDS             1013..1243
                     /locus_tag="VN178_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82028.1"
                     /translation="MRNPILAAILSLIVAGLGQIYNGQVGKGVIFIVIQLINGALTAV
                     LIGWILLPIVGLWAMIDAYMTAKHNNERYGFR"
     gene            1254..1445
                     /locus_tag="VN178_13505"
     CDS             1254..1445
                     /locus_tag="VN178_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82029.1"
                     /translation="MNREDLLTLESAVTAIEEAASAVAREVERDRLRETSLARLSTVE
                     AELNRSQLALEKIIQEETA"
     gene            complement(1602..2765)
                     /locus_tag="VN178_13510"
     CDS             complement(1602..2765)
                     /locus_tag="VN178_13510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HWS82030.1"
                     /translation="MELAGLNVLDVFIAVFVIAVILRGARTGFLAGVFSLVGVVVGVS
                     AGSRIALSLMPEDGNPIYGAGITLGSILAFAVLGEVIARTIGGSIRDRLSSPTSETLD
                     GFGGAVLGFALSLVLVWAVGVFALQSPTLAGLYPALRDSKILQALDERMPSGLLTQAV
                     AELEPLPQIHGPEPEVAAPEGSIVGDPDVQVASERTLRVTSRACGYGVEGSGWVAGRD
                     LVVTNAHVVAGQRATHVEVGRTGRRLPAKVVVFDEKNDIAVLRVNGLGLTPLRLAPPK
                     PGKDVAVIGFPENGPLDIEPARTGTTQLVSSGDAYDNGPVQRVVTSFRVYVRPGNSGG
                     PAVNKYGRVVSTIFASRTDSDNSGYGVPSRIVHRHLKVAADRTAPVSTEGCAN"
     gene            2945..3985
                     /gene="rlmN"
                     /locus_tag="VN178_13515"
     CDS             2945..3985
                     /gene="rlmN"
                     /locus_tag="VN178_13515"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWS82031.1"
                     /translation="MNAAEFLPDARVVLEERNEPPYRIRQVYKALTGSLVRDWKEATN
                     LPKELQAALSEEAPAAALTLERVSLASDGTRKYLFYTRDNHAIETVMIPEKTRRTVCI
                     STQVGCPMGCTFCATGLLGIKRNLKAREIAEQVFVCARDIAPERVTNVVVMGMGEPML
                     NYDATLGALRVLNDPEGFGLAARHMAVSTSGLVEKIRRFADEPEQFHLAISLHTPFGD
                     ERREMMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELLDH
                     PLYHLNLLRFNWTDTGFSATTKADAKRFLGHARELGLSATLRPSRGQDIDAACGQLAA
                     KDLRGRPAIPMA"
     gene            3989..4468
                     /locus_tag="VN178_13520"
     CDS             3989..4468
                     /locus_tag="VN178_13520"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWS82032.1"
                     /translation="MKGEPAERLDPRAITLWRITGALNMLPLLVGAGFAALALSRSGG
                     APLLFEVLPVLAILALAGVVVGVVPGLRWRRWRYEIRPDEVDLQRGIFWISRTLVPLA
                     RIQHVDTRQGPLQRRFGLSTVVLYTAAGPNQIPELSTPVAESARDRIAELTREQDEL"
     gene            4458..5909
                     /locus_tag="VN178_13525"
     CDS             4458..5909
                     /locus_tag="VN178_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWS82033.1"
                     /translation="MSSEKRLHPVAMLVGAIKTLRRWISGLVFPGVILLVSQGFSLRT
                     VTLFLLGFIVVAALAALWGFLSWRATIYAVTGNSFRLRQGVFQKSERTIPLEHVQSVD
                     TVQGIIQRAFGVYEVRIETAGGGASEPDASLAALDRDAAQTLRREIEGSRRESLEETT
                     GPTVLRRLSTRELLFAGATSGQIGVAFSVLAIGFQVLDNFFPEEYVRRLVETLAPNWL
                     MVVLILLPTGLILAWLLAIAGTILAYSGFTLSREGDYLYIKRGLLERREATIPLSRIQ
                     AVRISEGLLRQPFGLASLRVESAGYGEDSGVSTTLFPLLPHDEVEDLLAGAAPEFAGA
                     PAVEPLPRRALWRYVFRATVLYLIIAFAAVLVSFLVFQSALGLLGIFLIPPAAAYGWL
                     SYRDAGWAFEADRLVVRHRGLGRKTAIAPRGHLQSRGMVSSPFQRRVRLATFLAEVAS
                     GSGGSVLRVRDLDGGDARALVDDLGPEVRLEGA"
     gene            complement(5912..8776)
                     /locus_tag="VN178_13530"
     CDS             complement(5912..8776)
                     /locus_tag="VN178_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HWS82034.1"
                     /translation="MILERLFIENYKQLRDPIELFPPEGAIGIVGANGAGKSTLFESI
                     LWAFFGSRGGGPRFANESIPWSGGTTKDPTTVEVTLATDGGSFTVRRRLKSGSTSAEA
                     LDAQDRTIVSGTADVTRWVEENLLGMDRTAFEATFYARQKELRFFAHDEGISRVRKIS
                     RLLGISSVETAQTFLREDRNTLRSEARLLEGRLAEADLDSLQTELEIASKECDRLEAE
                     LEKVTEERDSAEEDLESARRTRASLEGTYREHSRLTSDLREAEADRRRAEDRRAEAEK
                     DLADLAAAEEELGRLQPQTARLPEVAAELEQLDEDRRLSEQRDRDRKEHLKAQRRISD
                     IEGEVSDDLEALDGEKDEVLPGFQDLFDLDGQELLAETLAVLERAAGELERAEGHHEE
                     LRKLAEDHEAYRVSEKAVREADEAYRAARDEAAGLAEDLEVLSGGEDLEQAERELREE
                     EDKLRELAAHHRGRANADEREAKNVDKAREAIDSGAEEHCPTCHREFESGEQAEISDT
                     LRRQAAAIRRRAARETEEAEKLSISAEKTGEKLERVTSNLKRWRELREKGVRAEDRVA
                     TRLEALGKARSDLEEIEARIAGTEAPGDDDLEASRARCDRLRALRDARPQVKTLATEH
                     SRLAERLAELVEELEGLSRVSYDAEAHRVKREEKDRLERALGRVEELERRLATRPEVE
                     RALQAARELGEKAETAAEKLRREISTLEFDESAYEASVERVTAAEERLSSLRDAREGL
                     GGEWKDADFRIERTKAELGRLDEDRKLANEKAAAASRMDEMDGLFTEFFRSLTARARP
                     MLEVEASDLVRELTDGRYERMEFDENYRVKLLDRFDDSYAIERFSGGESDVASLSARV
                     ALSRIVASRGGNTLGFLVLDEVFGSLDAGRRNNVLLALERLKRSFGQIFIISHVAEVQ
                     ESALVDEVWMLEEDEEGKSTVRRVNTPMMPATGAIAAGSA"
     gene            complement(8773..9933)
                     /locus_tag="VN178_13535"
     CDS             complement(8773..9933)
                     /locus_tag="VN178_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWS82035.1"
                     /translation="MVRIAHISDTHLGYTRYAKLHPETSRNQREVDVQEAYGRAVDAI
                     LERDVDLVIHSGDVFDSVRPATHVIISFLKQTFRITRQDIPYLVAAGNHETPRLRSTT
                     AALEYANLVNAISAHGFDIDYEPVEVDGATVGVTLVPHGAVFGTGAVTPSREADVNIL
                     VTHGLVPGLEARQHEMGEANLQPGMLEGGFDYIALGHYHDFHEHKPNAFYAGATERFG
                     FGEVDSKPGFAIVEFDSKGLESVEHVEIEARPMLDLKKISGQKMDAAELTEAVLDRTS
                     GVDLDGAIVRLRAYDVRRGVASGMDRELLRDLQRRCLNFSLEVHAEERPEDLKRDGSQ
                     AAVFGPLNEEFAAFVQERKERGELEAKFAEEFLEKGRAYLTRAASEEPESAA"
     gene            complement(9999..10466)
                     /locus_tag="VN178_13540"
     CDS             complement(9999..10466)
                     /locus_tag="VN178_13540"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HWS82036.1"
                     /translation="MDDNRVEDYWQSYLNTLPTDSPERDEEHIAEGWGDSPRMADELG
                     ALIASGIKTATCSALWEYEAEGEPLPRVGLKTIVLDGNGDPLCIVETTEVKVQPYDEV
                     DARFAYEEGEGDRSLAYWRDAHWRYFSRTLPNVGREPTTEMPLVCERFRVIYM"
     gene            complement(10512..12572)
                     /locus_tag="VN178_13545"
     CDS             complement(10512..12572)
                     /locus_tag="VN178_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82037.1"
                     /translation="MLHDSFLDLISGPIGRVVTSEDSPATAHEFFFWTAETEAAQNLD
                     IGHIVAAESEDATAVAVLDDPRRYSDLQSFLDDFYAYDGDPSLEALSERAEILVFRAR
                     VLATKHREERKKSKRPVRTGPVFFATSAAIEFALGTEDFSGHSIPMLLHENGNEENGS
                     PQRTPLYVDEEYLLGPEAGHLNITGMSGLSTKTSHALFTIASTFQTIKDKKVAALMFN
                     VKGADLLFLDKPVEVSAQDDPELAERYAKVGQRGLPREDREMYESLGIEIKPFDNLRI
                     FAPLRYGMESGERIVHAEGIPARKLNTLRNARGEDSRVYPIIWELGDVLPYAGYVFEP
                     SDMDDKFRGFIEELRDRGVRTTADFYVLLDEIEDYFEAAREEGKNVSSWNGHNHMTIA
                     KVRNRFKVLPNKCGGLLAHGRVEHGDLPRADGPFEDNEMRVVDISQLTGVPQDLIVTS
                     VVSKIWELAETGGLGVDKLIIFVDELNKYAPSGNSRTSSLKDTLVDISARGRHLNLTL
                     FGAQQFRSKVDDEVIGNAATSLYGRIGDEELTNASYRSFSGTTREELLQLEKGQLLLR
                     HAHYAVPVFGRFPRPPVLMGKQGTDIFGERRGDPASSVLAVMRNLTARPPAITTIRSE
                     IQGVPEEEVYEALDAVQAAQRTGHGAADPYKNFRWNLKRPARTGSGRRDASRILSGLD
                     RMRD"
     gene            complement(12573..13712)
                     /locus_tag="VN178_13550"
     CDS             complement(12573..13712)
                     /locus_tag="VN178_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82038.1"
                     /translation="MRAGSAVDDFVAKISQAIEGAGRKGIHLTAGAGSCDLEAQQFGE
                     SPEEQEPFPTGRPHHLESPVGARDPGGHRGISRLCYFLDGVQSSREIGRIGMSPVIVS
                     TVAATIVNRCGRRFSRLPMESPPVVVQAVILPRSAGDVGVEAFWDLLLAAGFYELGPD
                     EVPSSPHLVLDSAEYIADADPSDYVGMRERARVRVRALRERLEGGMLRQWEGDDRVLE
                     DADAWIAVDGQLENIRESNRRAIGLIKSVARPEFVGKDVGMLLDLEPGMRTTSFVPDW
                     QVSLDPGKQRTSWYLRMWPPQPGADALGSLMRVEAPRETGPDQVDEISRWILAERAPL
                     AKPDPRWPAMIYPIQYVEKILKPLVQGPERAYARLERQLALNGRN"
     gene            complement(13746..14330)
                     /locus_tag="VN178_13555"
     CDS             complement(13746..14330)
                     /locus_tag="VN178_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82039.1"
                     /translation="MTQEKGKGLDLPERVRKSLASAVADRGVQARVISTQGRESMRPP
                     QGHIIIAAGREEVEAVRGPARIVRRLEFYKTFYGPVVRLALSVYPMEGEPLSAGTALN
                     VSQVSGDAALTGLGRQKAIYIHFYSAIGDDLTYAFSKEIPNASEQRGETKKVHKMARD
                     AYTETPEERRSFRSAVGLAESQFELPVPEPDEEG"
     gene            complement(14327..15676)
                     /locus_tag="VN178_13560"
     CDS             complement(14327..15676)
                     /locus_tag="VN178_13560"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWS82040.1"
                     /translation="MDLRERVDGSREELLGELNEFLRMPSISAREEESTGFRDCAEWV
                     AGKLEEAGAEASIMETEGHPVVYAEIGSGEATLLSYGHYDVQPPEPLDLWESDPFEPT
                     IRDGGLYARGVADDKGDVLARIQALRLYAEEHNSLPFRLKFLIEGEEEIGSPNLAPFV
                     RSNADLLSADACLWEGSMKDEAGRPMIFCGTKGMAYVELRASGASHDLHSMYGGIAPN
                     PVWRLIQALRTIKDENGEITLDGLDALAEEPSEKDLEAISRIAFDDDALRESWSVESL
                     DRELSGQDALREMLLRPTANIAGIQSGYTGPGSKTIVPSEAFMKMDFRLVAGQSPGPV
                     VELIRSHLRERGFEDIEVVELHGVEPAKTPVDSPIVSTAIQTWQDLGEDEVLVYPTIG
                     GSGPTSLLVADLGIPTIMTGSVANTGSRLHSPNEWVRLDDYFEAITYFARFFERYGP"
     gene            15792..16307
                     /locus_tag="VN178_13565"
     CDS             15792..16307
                     /locus_tag="VN178_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82041.1"
                     /translation="MEKRPDRDLESRRRGGHGSWDSEDFFGLDVEKMSPKAQAVTGAV
                     TGGLILLAAALLIAFTQFWWLIFIFGWAVFPAFGVFARGVAGLVDTRSEPPAANARER
                     ELLEALQSSGELTPAQAAMETSLTVNEADEMLKDLAAKGHLDVRVRGGGLFYGLWESD
                     SEAVRKRLGEG"
     gene            16403..17143
                     /locus_tag="VN178_13570"
     CDS             16403..17143
                     /locus_tag="VN178_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82042.1"
                     /translation="MGERRIPETLEEAYRLRDSADSLQERDFYQEQVYHLRREEYRSR
                     GIRTEISSRRRERQSAIEFSREMFFLEVAEISRHGPGARETRAAQHEARKYATRLDTH
                     AFLKQLASPHSGAVELYRRNYLELLRMGKSPSEAYYLSRVIGLADEGEFRHALQSVRE
                     TGFPARGGVAYAVERSAIQIRDLPGGMILILLTFFASLFAFVWATQTWDNAKAGTIFF
                     VSLGLIAAGALMLISAIFIVTFIRSINK"
BASE COUNT         2798 a         5679 c         5442 g         3372 t
ORIGIN      
        1 gagtggcgca gctcgagcaa acggtcggca agacccagct ggtccatgat ctccatgacc
       61 gaagggtgca gcgtgtcccc gcggaactcg cggtcgaagt cggggtgagc ctcgagcagc
      121 gtcacgtcga cgccgctccg cgccagcaac aacgccagca ccgcgccgcc gggcccgccc
      181 cccacgacgc agcaggtcgt gcgcttgacg tcacggattg cctcggacgc tgctttcccg
      241 gtggcccgct ctgcgtggat ggccatggtc gacctcctat acctcgggtt cgagtccttt
      301 cagaaagacg ttgaccgccg tctgtatgtg cgcttcgtcg gtaaggccgt cttcccttat
      361 gaccgggaag aacagcttgc ccccggcttg ggggaggagc atgcctatga aggcgcgggc
      421 gctggaacgg acgtcgtgcg ggcgcagcct tccgagctcg acctggcgcg ccatgtaact
      481 cttcaagaac ccgagcacct tcttgatcac cgcgttgccg aacacctccg agacctcagg
      541 ccgcctcata gcctccccaa tgagcagtcg caccatcctc tgggtagtgc tgtcgtcgaa
      601 cgtcgaaaga tacctcctcg ctatgttcgg caacaccact tccggtggaa ggtcgaggag
      661 ccttgcagga tcccgcaccg cgcgcaaaac agggatcctg gaccccagaa cctccctgaa
      721 aagagcctcc ttgtcaggga aataccagta gatgagcgca ggcgactgca gactggccgt
      781 ccgcgcaatg ctcttgatcg tcgccccctc gaagcccttc tcagagaact cctcgaaagc
      841 cgcctccagg atctgcgccc gccggtctga tccctcagcc aagtcatccc ttcattgatc
      901 gtttaatcaa ccactatact cttgatagaa cgttcagtca agagccgttg aaacgagaaa
      961 tagattcgga cgtatacgtc atcgaagacg cagtcgacag cgaggagaaa cgatgcgcaa
     1021 tccaatactt gccgccatcc tcagcctcat cgttgcgggg ctgggccaga tctacaacgg
     1081 ccaggtcggc aaaggtgtga tcttcatcgt catccagctc ataaacggcg cgttgacggc
     1141 cgttctgatc ggctggatcc tactccccat cgtgggcttg tgggccatga tcgacgcgta
     1201 catgactgcg aagcacaaca acgaacggta cggcttcagg taatatccgt cccatgaaca
     1261 gagaagatct gctaacactg gagagcgccg ttacggccat agaggaggct gcctccgctg
     1321 tcgcccgcga ggtcgagcgc gaccggctgc gcgagacctc cctggcccgc ctctccacgg
     1381 tcgaggccga actcaacagg agccagctcg cgctggagaa gatcatccag gaagagacag
     1441 cgtagagcat ttcagcctgt cagcacgcgc cctgtcgggc gctcgtcagc atttcagctg
     1501 atcagctttc gcgctggtga gccttggctg acaaggcggc gcagccgccg ggctgaagtg
     1561 ctgacgagcg aaggctaaaa gcctgagcgt gctgaaatgc tctagttggc gcatccctcg
     1621 gtgctgacgg gggcggtgcg gtctgcggcc accttgaggt gacggtggac tattcgggag
     1681 gggactccgt agcccgagtt gtccgagtcg gtgcggctgg cgaatattgt cgagacgacc
     1741 cgtccgtact tgttgacggc cggaccgccc gagttgccgg gcctcacgta tacgcggaag
     1801 ctggtcacga cgcgctgcac cgggccgttg tcgtaggcgt cgccggagct gacgagttgc
     1861 gtcgtccccg tgcgggcagg ctcgatgtcg agcggaccgt tttcggggaa cccgatcacg
     1921 gcgacgtcct tgcctggctt gggtggcgca aggcgcagcg gcgtcagacc aaggccgttt
     1981 acgcgcagga cggctatgtc gttcttctcg tcgaagacga cgacctttgc gggaagacgc
     2041 cttcccgtcc tgccgacttc tacatgcgtc gccctctgcc cggcgacgac gtgggcgttg
     2101 gtgacgacca ggtcccggcc ggcgacccag cccgagccct ctacgccgta gccgcaggct
     2161 ctgctcgtca cccgcagcgt acgctcgctc gccacttgca cgtcgggatc tccgacgatg
     2221 ctaccttcgg gggccgccac ctccggttcg gggccatgga tctggggcag cggctccagc
     2281 tcggcgacgg cctgcgtgag gagcccggag ggcatgcgtt cgtcgagggc ctgcaggatt
     2341 ttggagtccc gcagggcggg gtaaagcccc gcgagggttg gtgactgcag ggcaaagacg
     2401 ccgacggccc aaaccagcac gagggagagc gcaaacccca gcaccgctcc gccgaatccg
     2461 tccagcgtct ccgaagtcgg gctggagaga cgatccctga tcgaaccccc tatcgtcctg
     2521 gcgatgacct ccccgagcac ggcgaaggcc aggatgctcc cgagggtaat acccgcgccg
     2581 tagatcgggt tcccgtcctc gggcatgagg gagagtgcga tcctcgaccc tgccgagacg
     2641 ccgacgacca cgccaacgag cgagaagaca cctgcgagaa agcctgtcct cgcgccgcgt
     2701 agtatcacgg cgatcacgaa aaccgcgata aacacgtcca gaacgttgag gccggcgagc
     2761 tccattgaca gattctaccg ccagtgcaag cgccttgccg cccgtgctcg agctcactgg
     2821 ccgtacagat ccggtgagtc ggcatcctgg caagagcgct acgatgaggt ccggtaggag
     2881 aaatcacgtt cgcggcttac cccgcgattt tgtgattggg cggccacggt ataatctctc
     2941 caccatgaat gcggcggaat tcttacccga tgcgcgggtg gtcctcgagg agaggaacga
     3001 accaccttac cgcatccgcc aggtctacaa agccctcaca ggttccctcg tccgggactg
     3061 gaaagaagcc acgaacctgc ccaaagagtt acaggcggcg ctctccgagg aggctccggc
     3121 ggccgccttg acgctggagc gcgtatccct ggcttccgac ggtacgagaa agtatctgtt
     3181 ctacacccgc gataatcacg ccatagagac ggtcatgatc cctgagaaga ccaggcgcac
     3241 ggtatgcatc tccacgcagg tgggatgccc gatgggttgt acgttctgcg ccacggggct
     3301 cttgggaatc aagaggaacc tgaaggcgcg tgagatcgcc gagcaggtct tcgtctgcgc
     3361 ccgcgacatc gcgccggaga gggtgaccaa cgtcgtggtc atgggtatgg gcgagccgat
     3421 gctcaactac gacgcgacgc tcggggcgct aagagtcctc aacgaccctg agggcttcgg
     3481 gttggcggcg aggcacatgg cggtctctac gagtggtttg gtagagaaga tccgtcgttt
     3541 cgcagacgag cccgagcagt ttcacctggc gatctcgctg cacaccccgt tcggggacga
     3601 gcggcgtgag atgatgccgg tagcagcccg ccataccatc cccgagctga tggatgctgc
     3661 ccgttacttc gtatcacgga cgcgccgcaa gctcttcttc gagtacacgc tgctcgccgg
     3721 cgtcaacgac cagccgcgcc acgtcgaggc cctggcggaa ctcctcgacc acccgctcta
     3781 ccacctgaat ttgctccgct tcaactggac ggataccggc ttttcggcca ccaccaaagc
     3841 ggatgccaag agattcctcg ggcatgccag agagctcggc ctctcggcga cgcttcgtcc
     3901 cagccggggc caggacatcg acgccgcctg cggccagctc gccgcgaagg acttgcgcgg
     3961 gcgtcccgcc attcccatgg cctgactcgt gaagggcgaa cccgcagaga ggctcgaccc
     4021 gcgggcgatc accttgtgga ggataacggg cgccctcaac atgctgccgc tcctggtcgg
     4081 agccggtttc gccgctctgg cgttgtcgcg ctccggagga gcgccgctcc ttttcgaggt
     4141 tctgcccgtg ctcgcgatac tcgcgctggc cggagtggtc gtcggtgtgg tacccgggtt
     4201 gaggtggagg aggtggcgtt acgagattcg gcccgacgag gtggacctgc aacgtgggat
     4261 cttctggatc agccgcacgc tcgtgccgtt ggcccgtatc cagcacgtgg acaccaggca
     4321 gggaccactg cagcgccggt tcgggctctc gaccgtggtc ttgtacaccg cggccgggcc
     4381 gaaccagatc cccgagctct cgacccccgt cgccgaaagc gcgagggacc gcatcgccga
     4441 attgaccagg gaacaggatg agctctgaga agcgcctcca ccccgtggcc atgctcgtcg
     4501 gggccataaa gacgctccgg cgctggatca gcgggctcgt cttccccggc gtaatcctcc
     4561 tcgtgagcca gggattcagc ctgaggacgg tgacactctt cctgctcggc ttcatcgtcg
     4621 tggcggcgct ggcggcgctg tgggggtttc tctcctggcg ggcgacgatc tacgcggtca
     4681 ccggcaattc tttccgcttg cgccagggcg tattccagaa gagcgaaagg accataccgc
     4741 tggaacacgt acagtcggtg gataccgtgc aggggatcat tcagcgcgcc ttcggcgtct
     4801 acgaggtcag gatcgagacg gcgggcggag gcgcgagcga gcccgacgcc tccctcgccg
     4861 cgctggaccg ggatgcggca cagacgctgc ggcgtgagat cgaaggttcc aggagggaga
     4921 gcctcgagga gaccacaggc ccgacggttc tcaggcggct ctcgacgcgc gagttgctgt
     4981 tcgccggggc cacgagcggt cagatcgggg tcgccttctc cgtgctcgcc atcgggtttc
     5041 aagtcctgga caacttcttc cctgaggagt acgtccggcg cctcgtcgag accctcgcgc
     5101 cgaactggct catggtggtc ctgatcctcc tgcccacggg tttgatcctg gcctggctgc
     5161 ttgcgatagc cggaactatc ctcgcctact ctggtttcac gctctcccgc gaaggggact
     5221 acctgtatat caagcgtggg cttctggagc gccgcgaggc gaccatcccg ctctcccgca
     5281 tacaggccgt tcgtatctcg gaaggtctcc tgcgccagcc tttcggcctc gcctccctgc
     5341 gcgtggagag cgccggctac ggcgaggact ctggtgtttc gaccacgctc tttcctctct
     5401 tgccccacga tgaggtggag gacttacttg ccggagcggc cccagagttt gcaggcgctc
     5461 cggccgtaga gccgctcccg cgccgcgccc tctggcgtta cgtattcagg gccacggtcc
     5521 tgtacctgat catcgccttt gccgccgtgc tggtctcttt cctcgtcttc cagtccgccc
     5581 ttggtctact cgggatcttc ctgataccac ccgccgccgc gtacggatgg ctgtcttacc
     5641 gggacgccgg ttgggcgttc gaagcggacc gcctggtcgt gcgccaccgg ggcctgggcc
     5701 gcaagacggc catcgcaccg cggggtcacc tgcagtcgcg cgggatggta agcagtccct
     5761 tccagcggcg tgtgcggctc gctaccttcc tcgccgaggt cgcctccgga tcgggcggtt
     5821 ctgtcctccg ggttagggat ctcgacgggg gagacgccag ggcgctggtg gatgatcttg
     5881 gccctgaagt ccgcctcgaa ggagcgtagg gttacgcgct accggcggcg atggcgccag
     5941 tagccggcat cataggggtg ttcacgcgcc tgacggtgct cttgccttct tcgtcttctt
     6001 ccagcatcca gacctcgtcg acgagggccg attcctgcac ttcggcgacg tgggagatta
     6061 tgaagatctg gccgaacgag cgcttgagac gctcgagcgc gagtaacacg ttgttgcgcc
     6121 ttccggcgtc gagactcccg aagacctcgt cgaggacgag gaaccctaag gtgttgccgc
     6181 cccgggaagc cactatcctc gagagggcca ccctggcgga gaggctggcg acgtcggact
     6241 cgccgcccga gaagcgctcg atggcgtagg agtcatcgaa gcggtcgagg agcttcaccc
     6301 ggtaattctc gtcgaattcc atccgctcgt agcggccgtc cgtgagctct cgcaccaggt
     6361 cggacgcctc gacctcgagc atcggtcgcg ccctcgccgt caggctgcgg aagaactcgg
     6421 tgaagaggcc gtccatctcg tccattcgcg aggcggcggc cgccttctcg ttggccagct
     6481 tcctgtcttc gtcgaggcgc cccagctcgg ccttcgtccg ctcgatacga aagtctgcgt
     6541 ctttccattc gcccccgagt ccctcccgtg catcccgcaa gctcgatagc cgctcctcgg
     6601 ccgccgtcac gcgctccacg gatgcctcgt aggccgactc gtcgaactcc agagtggaga
     6661 tctcacgccg caacttctcc gccgcagtct ccgccttctc tcccagctcg cgggctgctt
     6721 gcaacgccct ctcgacctcg ggccgcgtcg cgaggcgccg ctccagctcc tcgaccctgc
     6781 cgagcgcccg ctccagccgg tccttctctt cccgtttcac cctgtgagct tcggcgtcgt
     6841 aggagaccct ggagaggcct tcgagctcct cgacgagctc cgcgaggcgc tctgcgagtc
     6901 tggagtgctc ggtagcgagg gtctttacct gcggacgggc gtcgcgcaat gccctcagac
     6961 gatcgcagcg ggcgcgagac gcttcgagat catcgtcgcc gggggcctcc gtgcccgcga
     7021 tgcgggcttc gatctcctcc aggtcggatc tggccttccc gagcgcttcg agacgggtgg
     7081 cgactcggtc ctcggcgcgg acccccttct cgcgtaattc gcgccagcgc ttcaggttcg
     7141 aagtcaccct ctccagcttc tcgccggtct tttctgccga gatggatagc ttttcggcct
     7201 cttcggtctc gcgggccgcg cggcgacgga tcgcggccgc ctgcctcctc agggtgtcgg
     7261 agatctccgc ctgctccccg ctctcgaact ccctgtggca ggtgggacag tgctcctccg
     7321 cgccgctgtc gatggcctcc ctggccttgt ccacgttttt ggcctcccgc tcgtcggcgt
     7381 tggcccttcc cctgtgatgc gccgcgagct cccgcagctt gtcttcctct tcacgcagct
     7441 cccgctccgc ctgctccagg tcctcgccgc ccgaaaggac ctcgaggtcc tccgcgagcc
     7501 cggcggcctc atcccgcgcc gcccgatacg cctcgtcggc ctcgcggacg gccttctcgg
     7561 agactctgta ggcttcgtga tcctccgcta gcttccgtag ctcctcatga tggccctccg
     7621 ccctctccaa ctctcccgcg gctcgttcca gcacagcgag ggtctcggcg agcaactctt
     7681 gtccgtccag gtcgaagaga tcttggaagc ctggtaggac ctcgtccttt tccccgtcca
     7741 gcgcctccag atcgtccgag acttcgcctt ctatgtccga gatgcgccgt tgtgccttca
     7801 ggtgctcctt gcgatcgcgg tcgcgctgct cggagagccg gcgatcctcg tctagctgct
     7861 ccagctccgc cgccacctcc ggcagcctgg cggtctgggg ctgcaggcgc cccaactcct
     7921 cctctgctgc ggcgaggtcg gcgaggtctt tctcggcctc cgcacggcgg tcttctgcgc
     7981 ggcgcctgtc agcctcggcc tcgcgcagat cggacgtgag gcgcgagtgt tcgcggtatg
     8041 tgccctccag ggatgcgcga gtccggcggg cagactcgag atcctcctcg gcggagtcgc
     8101 gctcttctgt gaccttctcc agctcggctt ccagacggtc gcactccttg ctggcgatct
     8161 ccagctcggt ctgcagggag tcgaggtcgg cttctgccag gcggccttcg agtagcctcg
     8221 cttcggagcg gagggtgttg cggtcttcgc gcaggaaggt ttgggcggtc tcgacgctgc
     8281 tgatgccaag gagcctggag atcttacgca ccctgcttat gccctcgtcg tgggcgaaga
     8341 aacgaagctc cttctggcgg gcgtagaacg tagcctcgaa ggccgtccgg tccatcccca
     8401 gtaggttctc ctcgacccag cgcgtcacgt cggccgtccc gctgactatg gttctatcct
     8461 gggcgtccag cgcctcggcg ctggtcgagc cgctcttgag tcgccgccgc acggtaaagg
     8521 agccgccgtc ggtggccagg gtgacctcca ctgtagtggg gtccttggtc gtgccgccgc
     8581 tccagggaat agactcgttg gcgaagcgcg gtccgccgcc gcgggagccg aagaaggccc
     8641 acaagatgct ctcgaagagg gtcgacttgc cggcgccgtt cgcgccgacg atcccgatcg
     8701 caccctccgg cggaaagagt tcgatgggat ccctgagttg cttgtagttc tctatgaaga
     8761 ggcgctccag gatcacgcgg cgctctccgg ctcttcgctc gccgcccgcg tcaggtacgc
     8821 gcgccccttc tccaaaaact cttctgcgaa cttcgcctcg agctcgccgc gctccttgcg
     8881 ctcttggacg aacgccgcga actcctcgtt cagcggcccg aaaactgcgg cctgggagcc
     8941 gtctctcttc aggtcctcgg gccgttcctc ggcgtggact tccagggaga agttgaggca
     9001 ccggcgttgc agatccctca gcagctcccg gtccatcccg ctcgccaccc cgcgcctcac
     9061 gtcgtaggcc ctgaggcgca cgatcgcccc gtcgaggtcg accccggagg tacgatccag
     9121 caccgcctcg gtcagctccg cagcgtccat tttctggccg gagatcttct tcaggtccag
     9181 catcggacga gcctctatct cgacgtgctc tacgctctcg agtcccttgc tgtcgaactc
     9241 cacgatggcg aagccgggct tggaatctac ctccccgaac ccgaaacgct ccgtagcccc
     9301 ggcgtagaag gcgttcggtt tgtgctcgtg gaagtcgtgg tagtggccca aggctatgta
     9361 gtcgaatcct ccctccagca tccccggctg caggttcgcc tcgcccatct cgtgctggcg
     9421 cgcctcgagg ccgggcacca acccgtgagt gacgagtatg ttcacgtccg cctcccgcga
     9481 cggggtcacg gcccccgtcc cgaacacagc gccgtgcggg acgagcgtca cgcccacggt
     9541 cgccccgtct acttcgacgg gctcgtaatc tatgtcgaag ccgtgggcgg agattgcgtt
     9601 taccaggttc gcgtactcca gtgccgcggt cgtcgagcgc aggcgcggcg tctcatggtt
     9661 gcctgccgcg accagataag gtatgtcctg gcgcgtgatg cggaaagtct gtttcaggaa
     9721 gctgatgatc acatgcgtcg caggcctgac agaatcgaag acatcgccgg agtggatgac
     9781 aaggtccaca tcgcgctcca gaatagcgtc gacagcccgc ccataagcct cctggacgtc
     9841 cacctcccgc tggttacggc tcgtctcggg atggagcttc gcgtaccgcg tgtatcccaa
     9901 gtgagtatcc gagatgtggg ctatgcggac tatgttcctg cgatcctcac tactctagat
     9961 ccaagcaatt ctaaccgtgt gtggcgtggg tctgcattct acatatagat tacacggaat
    10021 cgctcgcata ccagcggcat ttccgtagtt ggctctctgc ctacgttcgg caacgtgcgc
    10081 gagaagtagc gccagtgcgc atcccgccag tacgctagcg aacggtcacc ctcaccttcc
    10141 tcgtaggcga agcgggcatc gacctcgtcg tagggttgta ccttgacctc cgtcgtctcg
    10201 acgatgcaca gaggatcgcc gtttccgtcc aggacgatgg tcttcagacc gactctcggc
    10261 aaaggctccc cttctgcttc gtactcccag agcgccgagc aggtcgccgt cttgatccca
    10321 gacgcgatca aagccccaag ctcatccgcc atacggggtg agtcgcccca tccttccgct
    10381 atgtgctcct catcgcgctc cggtgagtct gtcgggagcg tattcaggta gctctgccag
    10441 tagtcttcga cgcgattatc gtccacaacc agctccggta ataagccacg tacgggaagt
    10501 cttgaccagg gctaatccct catccgatcc aggccggaga gtattctgga cgcgtcgcgg
    10561 cggcctgagc ctgtcctcgc cggacgcttg aggttccagc ggaaattctt gtacgggtcc
    10621 gccgcgccgt ggcccgttcg ttgggccgcc tgcacggcgt cgagtgcctc gtagacttcc
    10681 tcttcgggga caccctgtat ctcggagcgg atggtggtta tggccggcgg tctggccgtc
    10741 aggttgcgca tgacggcgag gaccgaagat gccgggtcac cgcgcctttc gccgaagatg
    10801 tcggtgccct gcttgcccat gaggaccggc ggccgcggga agcggccgaa gacgggcacg
    10861 gcataatggg cgtgcctgag taggagctgg cctttttcga gttggagcag ctcttcgcgg
    10921 gtcgtgcccg agaaggagcg gtagctcgcg ttggtaagct cctcgtcgcc gatgcggccg
    10981 tagaggctcg tcgcggcgtt cccgatcacc tcgtcatcca ccttgctcct aaactgctgc
    11041 gccccgaaga gcgtgaggtt gaggtgccgg ccgcgggcgg agatgtcaac gagggtgtcc
    11101 ttcaaggacg acgtacgaga gttgccggaa ggggcgtact tgtttagttc gtcgacgaag
    11161 attatgagct tatccacccc gagcccgccc gtctcggcga gctcccagat cttcgatact
    11221 acgctcgtca cgatgaggtc ctggggtacc ccggtgagct gggagatgtc tacgacgcgc
    11281 atctcgttgt cctcgaacgg tccatccgct cgcggcaggt cgccgtgctc gacgcgcccg
    11341 tgcgccagga ggcctccaca cttgttgggg agtaccttga agcggttgcg gaccttggcg
    11401 atggtcatgt ggttgtggcc gttccaggat gagacgttct tgccttcctc gcgggccgcc
    11461 tcgaagtagt cctctatctc atcgagcaac acatagaagt ccgccgtcgt cctgacgccc
    11521 ctgtcgcgta gctcctctat aaaccccctg aacttgtcgt ccatgtccga tggctcgaag
    11581 acgtagccgg cgtagggcag cacgtcgccc agctcccaga tgatcggata gacccgggaa
    11641 tcttctccgc gcgcgttcct caaggtgttc agcttgcggg cggggatccc ctcggcgtgc
    11701 acgatccgct cccctgactc catcccgtaa cgcaacgggg cgaagatcct caggttgtcg
    11761 aagggcttta tctctattcc cagggactcg tacatttccc tgtcctcgcg tggcagtccc
    11821 cgctgaccga ccttcgcgta gcgctccgcg agctcgggat cgtcctgtgc gcttacctcg
    11881 accggcttgt ccaggaaaag taggtcggcg cccttcacgt tgaacatgag cgcggcgacc
    11941 ttcttgtctt tgatggtctg gaacgtgctc gcaatggtaa agagggcgtg gctggtcttg
    12001 gtagagagcc cgctcatgcc cgtgatgttc agatggcccg cttctggccc gagcaggtat
    12061 tcctcatcca cgtagagcgg cgtgcgctgg ggactcccgt tctcctcgtt gccgttctcg
    12121 tgcaggagca tcgggatgct gtgacccgag aagtcttcgg ttcccagggc gaactcgatg
    12181 gctgcgctcg tggcgaagaa cacgggtcct gtgcgtacgg ggcgcttgga cttcttacgc
    12241 tcttcacggt gcttggtggc gaggactcgg gctctgaaga ccaggatctc cgcccgctcg
    12301 gagagggctt cgagcgaagg gtctccgtcg taggcgtaga agtcgtcgag gaagctctgc
    12361 agatcagagt agcggcgcgg gtcgtcgagg acggcgacgg cggtggcgtc ctcggactcg
    12421 gcggcgacga tgtgcccgat gtccaggttc tgggcggctt ccgtctccgc tgtccagaag
    12481 aagaactcgt gggccgttgc cgggctatcc tcgctggtaa cgaccctgcc tatagggccc
    12541 gagatcaggt ccagaaaact atcgtgcagc atctagttcc tcccgttcaa tgcgagttgg
    12601 cgctccagcc gcgcgtaggc ccgctccgga ccctgtacca gcggcttcag gatcttctcg
    12661 acgtattgga tcgggtaaat catcgccggc cacctggggt cgggcttggc aaggggcgcc
    12721 cgctcggcga ggatccaacg cgatatctcg tcgacctggt ccggtccggt ctcgcgcggc
    12781 gcttcgacgc gcatgagcga gccgagggcg tcggcgccgg gttgcggcgg ccacatcctg
    12841 aggtaccagg aggtacgttg cttaccaggg tctaggctca cctgccaatc cggaacgaag
    12901 ctcgtcgtcc gcatcccggg ctccaggtcg agaagcatcc cgacgtcttt gcctacgaac
    12961 tcgggacggg ccacgctctt tatgaggccg atggcgcgcc ggttcgactc ccggatgttc
    13021 tcgagctgtc catcgaccgc gatccaggcg tcagcgtctt cgaggaccct gtcgtcaccc
    13081 tcccactgtc tgagcatgcc tccttccaga cgctccctga gcgccctgac cctcacgcgc
    13141 gccctctccc tcatacctac atagtcggac gggtcggcgt cggctatata ctccgcggag
    13201 tcgaggacga ggtgggggga ggaggggacc tcatcggggc ccagttcgta gaatcccgcc
    13261 gcgaggagca ggtcccagaa cgcttccacg ccaacgtcgc cggcgctgcg gggcaggatc
    13321 acggcctgca ccaccacggg cgggctctcc atcggcagcc gcgagaaccg tctcccgcag
    13381 cggttgacga tcgtggccgc caccgtggat acgatcacgg gggacatccc tatgcgcccg
    13441 atctcccgcg agctctgcac gccatccagg aagtaacaaa gccgcgagat gcctctgtgg
    13501 ccgccggggt ccctggctcc caccggggat tccaggtggt gcggacgccc tgtcgggaac
    13561 ggctcctgtt cttcgggaga ctcgccgaac tgctgggcct ccagatcgca agaccccgct
    13621 cccgcagtca agtggatgcc cttgcgtccg gccccttcga tggcctggct gatcttggct
    13681 acaaagtcgt ctaccgcact ccccgcccgc atacgtggca gtatacgcgg gcgcgccgcc
    13741 gaagactatc cttcttcgtc aggttcaggt acaggaagct cgaactgact ctcggcgagc
    13801 cctacggcag atctgaagct cctgcgctct tccggggtct ctgtataggc gtcgcgggcc
    13861 atcttgtgga ctttcttcgt ctcgccgcgt tgctcggagg cgttggggat ctctttggag
    13921 aacgcgtagg tgaggtcgtc gccgatggcg gagtagaaat gtatgtagat ggccttctgg
    13981 cggccgaggc cggtgagggc cgcgtcgccg ctgacctggg agacgttgag ggcggtgccg
    14041 gcggagagcg gttcgccttc catcgggtag acgctgaggg cgaggcggac tacgggcccg
    14101 tagaacgtct tgtagaactc cagccggcgt acgatgcggg cggggccgcg gaccgcttcc
    14161 acctcttctc tcccggcggc gatgattatg tgaccttgag ggggacgcat cgattcgcgg
    14221 ccctgcgtgg agatcacacg cgcctgcacg cctctgtccg cgacggcaga ggcgaggctc
    14281 ttcctgacac gttcgggtag atccaggccc ttgcccttct cttgtgtcaa gggccgtacc
    14341 tctcgaagaa ccgggcgaag taggttatgg cctcgaagta gtcgtccagg cggacccact
    14401 cgtttgggga gtggagccgg ctccccgtgt tcgccacgct cccggtcatg atggtcggta
    14461 tcccgaggtc ggcgaccagg agtgaggtcg gtccgctccc cccgatggtc ggatacacga
    14521 gaacctcgtc ttcgcccagg tcctgccacg tctgtatagc ggtgctgacg atgggcgagt
    14581 ccacgggcgt cttcgccggc tcgacgccgt gcagttcgac gacctcgatg tcctcgaagc
    14641 cacgctctct gaggtgggac cggatcaact ccactacagg accggggctc tggccggcga
    14701 cgagcctgaa gtccatcttc atgaacgcct ctgaaggcac gatggtcttg gagcctggac
    14761 cggtgtagcc cgactgtatg cccgcgatat tcgcggtcgg gcgcagcagc atctcgcgta
    14821 gcgcgtcctg gccggagagt tcccggtcga gagattccac actccaggac tcccgcaggg
    14881 cgtcgtcgtc gaaggcgatg cggcttatgg cttccaggtc cttctccgag ggttcttcgg
    14941 cgagggcgtc gaggccgtcg agtgtgatct caccgttctc gtccttgatg gtcctcaggg
    15001 cctggatgag gcgccagaca gggttagggg ctatgccgcc gtacatgctg tgcaggtcgt
    15061 gggaggcgcc gctggcgcgc agttccacgt aggccatccc cttggtgccg cagaagatca
    15121 tgggccgccc cgcctcgtct ttcatggagc cctcccagag gcaggcgtcg gcggagagga
    15181 gatccgcgtt ggatctgacg aagggcgcca gattggggct gccgatctct tcctcgccct
    15241 cgatcaggaa cttgagcctg aagggtagtg agttatgctc ctccgcatac agacgcaacg
    15301 cctgtatgcg ggcgagcacg tcgcccttgt cgtcagctac gccccgggcg tagaggccac
    15361 cgtccctgat agtaggctcg aacggatcgc tctcccagag atccaggggc tcgggtggct
    15421 gcacgtcgta gtggccgtag gagaggagcg tcgcttcgcc cgagccgatc tccgcgtaga
    15481 ccacgggatg tccttcggtc tccatgatgc tggcctcggc accggcctcc tccagcttcc
    15541 ccgccaccca ctctgcgcag tcgcggaacc ctgtgctttc ctcttcgcgg gcggagatcg
    15601 aaggcatccg aaggaactcg ttcaattccc cgagaagctc ttctctcgaa ccgtccacgc
    15661 gctcgcgcaa atccatcggc cagacctcct ttgtgactca ggtgaggtta gcactttcgc
    15721 gtccggcctt cgcggctcct atcgtggccc atcccctggc ttgctgatat attgggtccc
    15781 gaggcagacc tgtggagaag agaccggatc gcgaccttga gtcccgccgc agaggcggcc
    15841 acggaagttg ggattctgag gatttcttcg gcctggacgt ggagaagatg agtccgaagg
    15901 cccaggctgt gaccggcgcg gtgacgggcg gcttgatcct ccttgcagcc gcactcctta
    15961 ttgcttttac ccagttctgg tggctgatct ttatcttcgg gtgggcagtc tttccggcct
    16021 tcggcgtctt cgccaggggc gtcgccggcc tggtcgatac ccgctcggag cctccggcgg
    16081 cgaacgccag ggagcgcgag ctactcgaag ccctgcagag cagcggcgag ttgacgcctg
    16141 cgcaggcggc catggagacc tcgctcacgg tcaacgaggc tgacgagatg ctcaaggatc
    16201 tcgccgcaaa aggtcacctc gacgtcaggg tgcgtggggg agggctgttc tacgggttgt
    16261 gggagagcga ctccgaggct gtcaggaaga ggctcgggga agggtagtca tgtttctgtc
    16321 gcaacacgct catggtatca ttcaggaccc agaaggggtt attcggcgcg tgagatctgg
    16381 agtatagggt aaacactggt tcatgggaga acgtaggata cctgagacgc tagaggaggc
    16441 ttaccgcctg cgggattcgg ccgactcgtt gcaggagaga gacttctacc aggagcaggt
    16501 ctaccatctg aggcgcgagg aataccgctc gcgcggtata aggacggaga tctcctcccg
    16561 gcgccgcgag cgccagagcg ccatagagtt ctcccgcgag atgttctttc tggaggtcgc
    16621 cgagatctcc cgccacggcc ctggggcccg cgagacccgc gccgcccagc acgaggcccg
    16681 caagtacgcc acgcgcctcg acacccacgc cttcctgaag cagctcgcct caccccattc
    16741 aggcgccgtg gagctctaca ggcgcaacta cctggagcta ctcaggatgg gaaagtctcc
    16801 ctccgaagcc tattacctct cgcgggttat cgggctggca gatgagggag agttcaggca
    16861 tgccctccag agcgtgcgcg agaccggctt tccggcccgg ggaggcgtgg cctacgcggt
    16921 ggagcggtcg gcgatccaga tcagggattt gcccggtggc atgatcctga tcctcctcac
    16981 cttcttcgcc tctctgttcg ccttcgtgtg ggccacccag acctgggaca atgcgaaagc
    17041 gggcacgatc ttcttcgtct ctttgggcct tatcgccgca ggggcgctga tgttgatctc
    17101 cgcgatcttt atcgttacct ttatcaggtc gataaacaag tagcccctta cctcggggcg
    17161 cgatcatctc atgcgggggg ccgatccacg cgccggtgcc agggtccgag acgccaacta
    17221 cccggcggca acgccgcgag cagcagcaaa gagcactccg gcgccggcgt ctcctcgtgg
    17281 cgctgcctat c
//
LOCUS       DATMVF010000265         9822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_498081, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000265 DATMVF010000000
VERSION     DATMVF010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498081"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /gene="glmU"
                     /locus_tag="VN178_13575"
     CDS             complement(<1..1367)
                     /gene="glmU"
                     /locus_tag="VN178_13575"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWS82043.1"
                     /translation="MSERSPIFAVILAAGKGTRMKSNRAKVLHTLCGVPMVNYVIEAT
                     RPLLPEGLFVVVGHQAELVEAVLPEDAKPVMQEEQRGTGDAVRVALDALEGEDEGVLL
                     VVNGDGPLISARTLGELVERHSSAGVGATVLVAELEDPSGLGRVVEDAGVVRIVEERD
                     ATESERRIRLVNLGLYAFKLSEIRDTIGGLTSENRGGELYLTDVLETIGKRSRAVTYR
                     LKNPEEANLVNDRSQLARAEEILRRRILDAHMRDGVTVRDPTSTHIEASVEIGRDTVI
                     LPGSLLRGETRIGTDCVIGPSTDLEDTRVGDGALVEHSVGRGAEVGDGASVGPYSFLR
                     TGTVLEPGVKVGAYCEVKNSRVGAGSKVPHLSYVGDAQIGTDVNLGAGTITANYDGEN
                     KHRTMVGDGAHTGVNTNLIAPVSVGEDAYTGAGSVVNKDVPAGKLAVGAPARAIRESP
                     QRRRKR"
     gene            complement(1427..1497)
                     /locus_tag="VN178_13580"
     tRNA            complement(1427..1497)
                     /locus_tag="VN178_13580"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1463..1465),aa:Gln,seq:ttg)
     gene            complement(1614..2486)
                     /gene="ispE"
                     /locus_tag="VN178_13585"
     CDS             complement(1614..2486)
                     /gene="ispE"
                     /locus_tag="VN178_13585"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWS82044.1"
                     /translation="MREVRLRAFAKVNYALDVLGLRADGYHEISTVMQSISLADEVEL
                     RRATGGFDLSLVPEKVRIGAQEQNTTFLAWKALRRLTGKELPVEVTLRKKIPAGAGLG
                     GGSADAAAVLVGLNELFGLGLRVDELRVIGAGIGADVPFCVSGGTALGEGIGEILTPL
                     PAPPAHLLVVAKPLSSADTGKIYHAFDEAGTQSTHSVEPVVSALRSGSIPDLASAIGN
                     DLTPVTMDIVPEVAELEQTLLASGALGASMSGSGTAVFGIFDDEMTARGAQDTVDVPF
                     IGVYEPVSSGVEIV"
     gene            complement(2486..3262)
                     /gene="rsmA"
                     /locus_tag="VN178_13590"
     CDS             complement(2486..3262)
                     /gene="rsmA"
                     /locus_tag="VN178_13590"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWS82045.1"
                     /translation="MSELGPPKKRFGQHFLKDPNTARIVASGVTEDDVVLEIGPGRGF
                     LTTFLAERASLVHAVELDPDVLPSLREAVRNRDNVIVHEGDALRFDYPALDPPPNRLA
                     ANLPYNIASPLVLRLLEEVETLRTLRFMVQLEVARRMAAERGSKDYAAYAVLAQLLAR
                     VSISHRVPPTVFDPPPRVHSAVVEMDRREPPDDYQGIKRLVLSAFRSRRKRFVNNLPE
                     PARGPAPRVLETLGYGPNVRAEELAPEDFAALYAALSWAL"
     gene            complement(3269..4027)
                     /locus_tag="VN178_13595"
     CDS             complement(3269..4027)
                     /locus_tag="VN178_13595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWS82046.1"
                     /translation="MLVDSHTHLLRLEISPEDAVRSAAEVGVVAVVNVGTDVEDSQRG
                     AELAAVLPNVYSTAGIHPHNAGTYTAADLEALAELSESPGIVALGEVGLDYYRSEWSE
                     EVQRALFEDVISLANDTRLPLVIHSREAFADTMACLGSARVPVVLHCFEGGEREVREA
                     RERGYYVGLAGNVTYRDSETARVLRLIDEERLLMETDAPYLSPQPVRGKKNSPENVVH
                     TAGFVAERLGMEDEELATLTTRNARNLFGLPLEV"
     gene            complement(4093..4893)
                     /gene="rsmI"
                     /locus_tag="VN178_13600"
     CDS             complement(4093..4893)
                     /gene="rsmI"
                     /locus_tag="VN178_13600"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWS82047.1"
                     /translation="MPLTVIPTPIGNLEDITLRALRYLREADLIACEDTRRTGRLLAH
                     YEIEGNLISYHEHNEKRLTLELAERAKTERVALVTDAGTPLVSDPGYRLVRACLEVGV
                     EIEALPGPSALTTALVASGLPADTVVFAGFPPRKGRDRKVLLERVATEPSTFVLFESP
                     RRLGKTLRDLPSETPVAVCRELTKLYEEVFRGTAEQAARRFSGNVKGEIVLVTRGGTA
                     RTEESIDDVVRRARDYVAEGESPSRAAQKAARESGHRRGEIYERLVKT"
     gene            complement(4897..5850)
                     /locus_tag="VN178_13605"
     CDS             complement(4897..5850)
                     /locus_tag="VN178_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWS82048.1"
                     /translation="MNAVGVDVGGTKIAAGVVTPEGKILDEVRYPTPHTPETLVETIS
                     RAISEVGDGPRVGGVCLAVPGLILSQANKVVFSPNLRAIEGIPLKDELGPKIGMPLTV
                     ENDGNAAAWGEFRFGVGSEADHLVFVTLGTGIGGGVITQGILMRGAQGSGGELGHVTI
                     HATGPRCACGNHGCLEALASGTAIGRRARELAVEQPSSALGRLAARRQVLGEDVTELA
                     REGDEVALSVLDEAGRWLGVGLAGFVNIFNPEVVAVGGGVMEAGELILKAARKEVILR
                     ARPPSRDLADVKVATLGPRSGILGAAALARDASSGEYLLGG"
     gene            complement(5847..7511)
                     /locus_tag="VN178_13610"
     CDS             complement(5847..7511)
                     /locus_tag="VN178_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HWS82049.1"
                     /translation="MARWRLLEPDADVVSELAREADVDLLCARVLANRGVAPGAAADF
                     LSPSLAAIGMPDEGRWSVAAQRVAKAIEGGERIGVFGDYDTDGITSAAVLFLALSHYT
                     DNVTVGLPTRQTGYGLLEPYVRDLFAEGVDLLITADCGISNRREVALANDLGMEVVVT
                     DHHIPPEDPPDTAVAVLDPKLWDPDDPLAGVGVAWKFAWAVARRLGDAEGRKRLGRLL
                     DLVSVGTVVDIAPLVGDNRALARMGLKYINRGLAAGEARPGIEALVKVAGVRGEIDED
                     DLGWRLGPRINSIGRIKNPRPALELLLTDDRREAFRIASLLNQLNAERQRRTKFAVER
                     AMAEVDPEQDFKVVVTEETGGIAGLIAGRVAGATGRPAAILTRRVDDSYGGSARAGET
                     DVDLYGALYAVRHLMGEWGGHRKAAGLSVNPGNFDAFVAGVNEAVRSQSEERPEIFVP
                     AIEVDAEIPLGSVSNGLLEWHEMLAPFGSGNDRPIFITENLRIAGSRKIWEGMNLLNL
                     EGGIKAKMAGEPDELPSGPFDAAYTVTRSRYSGKAELEIVDWKAGG"
     gene            complement(7712..8923)
                     /locus_tag="VN178_13615"
     CDS             complement(7712..8923)
                     /locus_tag="VN178_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HWS82050.1"
                     /translation="MRVFLALTALQAVIVGVLVALAELRKRRQGLREGFPWEDQPEIK
                     MESGEVRLKIFAYGVSLYEAMLDEIEAAQDEIFVGTFIWKGDEVGRRFVDALARKAGE
                     GVDVYVVFDGLANVFVPPSFKRFPENIHRLHFRPLSGPAKILNPRNVFRFHRHVMSVD
                     GRVAFLGGYNIGSLYAAGWRDTHVRVRGDAAREVDNAFVDFWNAHRTEELPRIAPNKE
                     RDWNPAVRVRRNDPYMRIFPIREMYIEAIDRANSHVYLTHAYFVPDRAMKAHLIDATQ
                     RGVEVQLLVPEHSNHVTVDWLARRHFHDLLKNGVRIFLYRHIMIHSKTATADGLWSTI
                     GSANIDRLSMLGNYELNIEVYSRRLAAQMESMFEMDKTNAREVTLEEWERRPLPAKVV
                     ERTLASLSPLV"
     gene            complement(9274..>9822)
                     /locus_tag="VN178_13620"
     CDS             complement(9274..>9822)
                     /locus_tag="VN178_13620"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HWS82051.1"
                     /translation="DPGEISLPSKSGEAMLEELAPRIVLALDEDFLVALAKGYPPLRR
                     AVCEEIVRNNARQNAVIGALPIPGADMPVMTANQARMVLNIAAAYGEELSMDRARELL
                     GVLAAGFGLRALSRQAVKLVPVAGWAASAVIAYAGTVAMGRTGILYFERGKQEVGAKE
                     MARIRTRAIEEGKAFVSRMRRK"
BASE COUNT         1683 a         3331 c         3007 g         1801 t
ORIGIN      
        1 cgtttcctac gccgctgggg agactcccgg atcgcacgcg ccggagcccc cacggccagt
       61 ttgccagccg gtacgtcttt gttgaccacg gacccagccc ccgtgtaagc atcctcccct
      121 acgctaacag gcgcgataag gttcgtgttc accccagtat gggcaccgtc tcccaccatg
      181 gtgcggtgct tgttctcacc gtcgtagttc gccgttatgg tcccggcccc aaggttcacg
      241 tcggtcccga tctgggcgtc ccccacgtac gagaggtgag gaaccttgct ccccgcccct
      301 acgcgagagt tcttgacctc gcagtaagcc ccgaccttga cccccggttc gaggaccgtg
      361 ccggtccgca ggaaagagta gggaccgacg ctcgccccgt cgccgacctc ggcaccgcgt
      421 ccgaccgagt gttcgacgag ggcaccgtcc ccgacacgcg tgtcctcgag gtccgtggag
      481 ggcccgatca cgcagtccgt accgatcctc gtctccccgc gcagcaggct tccaggcagg
      541 atcacggtgt cgcgaccgat ctcgaccgaa gcctctatgt gcgtcgaagt cggatccctc
      601 accgtcaccc cgtccctcat gtgggcatca aggatgcggc gcctgaggat ctcttcggcc
      661 cgagctagct gggagcggtc gttgacgagg ttcgcctcct ccgggttctt gagcctgtag
      721 gtaacggcgc ggctgcgctt tccgattgtt tcgagtacat ccgtgaggta cagctcgcct
      781 cccctattct ccgaggtgag gcccccgatc gtatccctta tctccgagag cttgaaagcg
      841 tagaggccga ggttgacgag cctgatccgt cgctctgact cggtcgcgtc cctctcctcg
      901 actattctga ccacgccggc gtcctcgact accctcccga ggccgctcgg gtcctccagc
      961 tcggccacga ggaccgtcgc cccgacgccg gcggaactgt gccgctcgac gagttccccg
     1021 agggtcctgg ccgagataag cggaccgtcg ccgttgacca cgagaagtac gccttcgtct
     1081 tccccttcga gcgcgtccag ggcgacgcgc acagcatcac cggtcccgcg ctgctcctcc
     1141 tgcattacgg gcttcgcgtc ctctggcagg accgcttcga caagctcggc ctgatggccc
     1201 acgacgacga acaggccctc gggtaatagg ggcctggtgg cttcgatcac gtagttgacc
     1261 atcgggacgc cgcacagggt gtgcagaacc ttcgcccggt tcgacttcat cctggtgccc
     1321 ttgccagcgg ccaggatcac ggcgaagatc ggcgacctct cagacatgta cccgcaccac
     1381 tcctatcgtt cgtctacccg gcggccgcat gccacacgct cctcggctgg ggcggcagga
     1441 ttcgaacctg cgatcccggg accaaaaccc ggtgccttgc cacttggcca cgccccagta
     1501 caggcccgcg cagattatac cggagcgcca tttcgccgtc aggaataggg tggatctcga
     1561 actgccaggc gttgggtccg ggtccggacg gtacgcccat gttcgcatcc tgctcagaca
     1621 atctccaccc cgctagagac cggctcgtag acaccgatga aaggaacatc gaccgtatct
     1681 tgtgcacccc tggcggtcat ttcgtcgtcg aagatgccga agaccgccgt cccgctgccg
     1741 ctcatcgaag ctcccaacgc ccctgatgct agaagcgtct gctcgagctc ggctacctct
     1801 gggacaatgt ccatggtcac gggtgtcaga tcgttgccga tagccgaggc gagatcaggg
     1861 atgctccccg atcgcaacgc cgagacgacg ggctcgacgg agtgcgtgct ctgtgttcct
     1921 gcctcgtcga aggcgtggta gatcttaccc gtgtccgcgc tactgagcgg tttggcgacg
     1981 acgagaagat gggccggtgg cgcaggaagt ggtgtgagga tctccccgat gccttcgccc
     2041 agggccgtcc cccccgagac gcagaaaggc acatcggccc cgatcccggc cccgataaca
     2101 cgcagctcat ctactctcaa cccgagaccg aatagctcgt tcagacccac gaggaccgcc
     2161 gcagcatcgg ccgagccgcc accgagtccg gccccggccg ggattttctt gcgcagcgta
     2221 acctctacag ggagctcctt accggttagt ctccgtaacg ccttccaggc caggaaggta
     2281 gtgttctgtt cctgcgcccc gatcctcacc ttttctggca ccagcgagag atcgaaacct
     2341 ccggtggcgc ggcgcaactc cacctcatcg gcgagcgaga tgctctgcat gacggtactg
     2401 atctcgtggt aaccgtccgc gcgaagcccg agaacgtcga gcgcgtaatt gaccttggcg
     2461 aacgccctga gccggacctc ccgcattaca aggcccagga tagtgccgcg tacaaggccg
     2521 cgaagtcttc cggcgccagt tcctcggccc tcacgttcgg gccgtaccca agggtctcca
     2581 ggacccttgg agcgggtccg cgggccggct ccggcaggtt gttgacgaac cgtttgcggc
     2641 ggcttctgaa cgcgcttagc acgaggcgct ttatcccctg gtagtcatcc ggcggttctc
     2701 tgcgatccat ctcgacgacc gcggagtgca ccctgggcgg cgggtcgaac accgtcgggg
     2761 ggaccctatg cgagatgctc acccgcgcca gaagctgcgc gagcacggcg tacgccgcgt
     2821 agtccttgga accacgctcc gccgccatcc tgcgcgccac ctcgagctgc accatgaacc
     2881 gcaacgtcct caacgtctcc acttcctcga gtaaccgcag aacgagcggc gaggcgatat
     2941 tgtacggaag gtttgcggcc agcctgttcg gtgggggatc gagtgcagga taatcgaagc
     3001 ggagggcgtc tccttcgtga acgataacgt tgtccctatt ccgaacggcc tcacgcaagc
     3061 tcgggagcac gtcgggatcc agctccactg catgcaccag gctcgccctc tcggcgagaa
     3121 aagtcgtcag gaagccccgt cctgggccga tctcgaggac cacatcatcc tccgttacgc
     3181 ccgaggccac tatcctggcg gtgttcgggt ccttgaggaa atgctgtccg aagcgttttt
     3241 tcggcgggcc cagctcggac accgaagctc acacctctag aggcaggccg aagagattgc
     3301 gggcgttacg ggtggtgagg gtggccagtt cctcatcctc catcccgagc ctttcagcaa
     3361 cgaagccggc cgtgtgcacg acgttctcgg gcgagttctt cttgccgcgc acgggctggg
     3421 ggctcaggta aggtgcgtcc gtctccatga ggaggcgttc ctcgtctatc aggcggagca
     3481 ccctggccgt ctcgctgtcc ctgtacgtga cgttgcccgc gaggcccacg tagtagccgc
     3541 gttcccttgc ttccctgacc tcgcgctcgc caccctcgaa gcagtgcaag acgaccggta
     3601 ccctggcgct accgaggcag gccatcgtat cggcgaaggc ttcgcgggag tgtataacca
     3661 gcggcaagcg cgtatcgttg gcgagggaga tcacatcctc gaacagcgcc cgctgcacct
     3721 cttctgacca ctccgaacgg tagtagtcga gcccgacctc accaagggcc actatccccg
     3781 gcgactccga gagttcggcc agcgcttcga ggtcggccgc ggtgtacgta ccggcgttgt
     3841 ggggatgtat ccccgccgtc gagtagacgt tcggcaacac cgccgcgagt tcggcacccc
     3901 tctgcgaatc ctcgacgtcg gtccccacgt tcaccacagc aacgaccccg acctccgcgg
     3961 cgcttctcac cgcgtcttcc ggcgagatct cgagccgcaa taagtgcgtg tgcgagtcca
     4021 cgagcattgc gcctatcccc tcttccccag agccatcagc gaagggcatt ataaaggggc
     4081 cggatatctc cttcaggtct tgaccagccg ctcgtagatc tcaccgcgac gatgtcccga
     4141 ctcgcgggcc gctttctgcg ccgctcgcga aggactctct ccctcagcca cgtagtcccg
     4201 tgcccgtcgg actacgtcgt ctatgctctc ttcggtcctt gccgtaccgc cgcgggttac
     4261 gagcacgatc tcgcctttca cgttccccga aaaacgtcgg gcggcctgct cggccgtccc
     4321 cctgaagact tcctcgtaga gcttggtaag ctccctacac acggcgaccg gggtctcgga
     4381 aggaagatcc cttagcgtct tcccgagccg gcgtggggac tcgaagagta cgaaggtaga
     4441 cggctcggtg gcgacacgct ccagcagaac cttgcgatct ctccccttgc gtggcgggaa
     4501 gccggcaaag acgaccgtat ctgcgggcaa ccccgaggca acgagcgcgg tggtgagggc
     4561 cgaggggccg gggagggctt ctatctcgac gccgacctct aagcaggcgc gcacgagcct
     4621 gtatccggga tctgagacga ggggggtgcc ggcgtccgtg acgagggcaa cgcgctccgt
     4681 cttcgcccgt tcggcaagct cgagggtgag ccgcttctcg ttgtgctcgt ggtatgagat
     4741 gaggttcccc tctatctcgt agtgggcgag caggcgtcct gtacgccttg tgtcctcgca
     4801 ggctatgagg tcggcctcgc gcaggtagcg gagggcgcgg agggtgatgt cctccaggtt
     4861 gcctatggga gttgggatca ctgttagggg catctctcag ccgccgagca ggtactctcc
     4921 ggatgaagca tccctggcga gcgccgcggc gccgagaatg ccggacctgg gaccgagggt
     4981 agcgaccttg acgtcggcca ggtcgcgtga agggggcctg gcgcggagga tgacttcctt
     5041 gcgggccgcc tttagtatga gctccccggc ctccatgacg cctccgccga cggcgaccac
     5101 ctcggggttg aagatgttta cgaacccggc gaggccgacg ccgagccacc gccctgcctc
     5161 gtcgaggacc gagagcgcca cctcgtcacc ttcgcgggcg agctctgtga cgtcctcgcc
     5221 gagtacctga cgcctggcgg ccagccgtcc cagcgccgag ctgggttgtt cgaccgcaag
     5281 ctcgcgggcg cggcggccta tggcggtacc cgaggcgagg gcctccaggc agccgtggtt
     5341 gccgcaggca cagcggggtc ctgtagcatg gatggtcacg tgaccgagct cgccccctga
     5401 gccctgcgcg ccgcgcatga ggatgccctg cgtgatgacg ccgcctccta tccctgttcc
     5461 cagcgtgacg aagaccaggt ggtcggcctc gcttccaacc ccgaaccgga actcgcccca
     5521 cgccgccgca ttgccgtcgt tctcgaccgt gaggggcatc cctatcttcg gcccgagttc
     5581 gtccttgagc ggaatcccct ctatcgcccg caggttcggg gagaagacga ccttgttcgc
     5641 ctgggagagg atcaaacctg gcacggcgag gcacacccct ccgacccggg gaccgtcccc
     5701 gacctcggag atcgccctgg agatggtctc gaccagcgtc tcgggcgtgt gtggcgtggg
     5761 gtagcggacc tcgtccagga tctttccctc gggggtaacg accccggcgg cgatcttggt
     5821 gcccccgaca tcgacgccga cggcgttcac ccgcccgcct tccagtccac tatctccagc
     5881 tcggccttcc cggagtaccg gctgcgggtg accgtataag cggcgtcgaa cggcccagag
     5941 ggcagttcgt cgggttcgcc ggccatcttg gccttgatcc cgccttcgag gttgaggagg
     6001 ttcatgccct cccagatctt gcgcgagccg gcgatccgca agttctccgt aatgaagatc
     6061 ggacgatcgt tcccgctacc gaaaggggcg agcatctcgt gccattcgag cagtccgttg
     6121 gagacgctcc caagcggtat ctcggcatcg acctctatgg cggggacgaa gatctccggc
     6181 ctctcttcgc tctgggacct gacggcctcg ttgacgccgg cgacgaaagc gtcgaagttg
     6241 ccagggttca cggagaggcc ggcggccttg cggtgcccac cccactctcc catcaagtgg
     6301 cggaccgcgt agagggcgcc gtagaggtct acgtcggtct cgccggcccg ggccgatccg
     6361 ccgtaggagt cgtcgaccct gcgggtcagt atggccgcag gccttcccgt ggcgccggcg
     6421 acgcggccgg cgatcaaacc cgcaatccct cccgtctctt cggtcacgac gaccttgaag
     6481 tcctgctcgg gatccacctc cgccatggcc cgttcgacgg cgaattttgt ccgccgctgg
     6541 cgctccgcgt tgagctggtt caggagggag gctatcctga aagcctcccg acgatcatcg
     6601 gtcagaagca gctcgagagc gggacgcggg ttctttatcc tgcctatgga gttgatgcgc
     6661 ggcccgagcc tccacccgag gtcgtcctcg tcgatctccc ccctcacccc tgccaccttg
     6721 accagcgcct cgatgccggg acgtgcttct ccggcagcga ggcccctgtt tatgtacttg
     6781 agacccatgc gggcgagcgc cctgttgtcc ccgacgagcg gcgctatgtc gacgacggta
     6841 ccgaccgaga cgaggtcgag aagtcgcccc agacgtttcc tgccctcggc gtcgccgagc
     6901 ctgcgggcta cggcccaggc gaacttccag gcaaccccca ccccggccag ggggtcgtca
     6961 gggtcccaga gcttcgggtc gagtacggcg accgccgtat ctggaggatc ttccggcggg
     7021 atgtgatggt ccgtcacgac cacctccatc ccgagatcgt tcgccagcgc cacctcacgc
     7081 ctgttggaga tgccgcagtc ggcggtgatc aggaggtcaa caccctcggc gaagaggtcg
     7141 cgcacgtaag gttccaggag accgtagccg gtctggcggg tcggcaggcc gacggtgacg
     7201 ttgtccgtgt agtgcgagag cgcaaggaac agcacggcgg ccgaggtgat gccgtcggta
     7261 tcgtagtcgc cgaagactcc gatcctctcc ccgccctcta tggccttcgc aaccctctgt
     7321 gcagcgacgc tccaccggcc ttcatcgggc atcccgatag cggcgagcga gggggagagg
     7381 aagtccgcgg ccgcaccggg cgcgacgccg cggttggcca gcaccctggc gcacaggagg
     7441 tccacatcag cctcccgcgc aagctcagac accacatccg catccggctc caggagccgc
     7501 cagcgcgcca aagagtcctc cttacacgaa caatcccaaa ataagggtat cgtcattata
     7561 caaaccgaac ttaatgcggc tcgctggagt ctcggcctcc agcgagccgc gccttcagct
     7621 ttcagcttca agcaatcagc caaagaccaa tagctgacgg ctgagagctg acagcgcggg
     7681 tcgcaaaagg tcaaaccttt tgcgacccgc cctagaccag tggactcaga ctggcgaggg
     7741 tacgttcgac gaccttggcg gggagtggcc ggcgttccca ctcctccagg gtcacctctc
     7801 gggcgttggt cttgtccatc tcgaacatgc tctccatctg ggctgcgagc ctgcggctgt
     7861 agacctcgat gttcagctcg tagttgccga gcatgctcag gcggtcgatg ttcgcgctgc
     7921 caatcgtcga ccacagaccg tctgcggtgg cggtcttgga gtggatcatg atgtgcctgt
     7981 aaaggaagat cctgacaccg ttcttgagaa gatcgtggaa gtgacgccgc gcgagccagt
     8041 ctacggtgac gtggttggag tgctctggaa cgagcagctg tacttcgacg ccgcgctgcg
     8101 tcgcgtctat caggtgcgct ttcatcgccc ggtctgggac gaagtaggcg tgggtcaggt
     8161 agacgtggga gttggcgcgg tctatcgcct cgatatacat ctcccttatg gggaagatcc
     8221 tcatgtaggg gtcgttgcgc cgcaccctta cggcagggtt ccagtcgcgc tccttgttgg
     8281 gggctatcct gggcaactcc tccgtgcggt gggcgttcca gaagtcgacg aaagcgttgt
     8341 ccacctccct ggcagcgtcg ccgcgcaccc tgacgtgcgt gtcgcgccaa cccgccgcgt
     8401 aaagggaccc gatgttgtag ccgccgagga aagcgaccct gccgtccacg ctcatcacgt
     8461 ggcggtggaa tctgaagacg ttcctagggt tcagtatctt cgccggaccc gaaaggggac
     8521 ggaagtggag gcggtggata ttctcgggaa acctcttgaa cgaaggcggc acgaaaacgt
     8581 tggccaaccc gtcgaagacg acgtagacat caacaccctc gcccgccttt cgggccagcg
     8641 catccacgaa acggcgtccg acctcgtcac ccttccatat aaacgtcccg acgaagatct
     8701 catcctgcgc ggcctcgatc tcgtccagca tcgcctcgta gaggctgacc ccgtacgcga
     8761 agatcttcag ccggacttcg cccgactcca tctttatctc gggctggtct tcccacggaa
     8821 acccctcccg cagaccttgg cggcgcttgc gcaactcggc gagcgcgacg aggacgccaa
     8881 caataaccgc ctgcaacgcc gtcaaagcga ggaaaaccct caatcccagt ctgcgcaacg
     8941 tcgagagccc ggcacgcacc tctgatagaa cccgccgcaa gacagagaac atagattcga
     9001 ttatatagta ggcttcaggc ttcaggcttc aggcaacagg taggtcgttc ttggagccgc
     9061 ggggtacgac ctgccgccgt cattcccgct ccccgctttc gcggggacaa gcttcgcggg
     9121 aatgacggat tatcgggtcg cggggataac gcttacgccg gggacgagac tacagaaggt
     9181 acgctcggcg tgcgctttgc acacacgacc acacgcattg cgtatcagca caagaaggcc
     9241 ggtacctgaa acctgatgcc tgtgacctga aacctatttt cggcgcatac gcgagacgaa
     9301 ggccttcccc tcctcgatgg cccgagtcct gatcctggcc atctccttgg caccgacctc
     9361 ctgtttgccg cgttcgaagt agaggatgcc ggtacggccc atcgccacgg taccagcgta
     9421 ggcgatgacc gcggaggcgg cccatcccgc cacaggcacg agctttacgg cctggcggct
     9481 cagtgcccta aggccgaaac cggcggcgag cacaccgagg agctcccgcg ccctatccat
     9541 cgacagttcc tccccgtaag cggccgctat gttgagcacc atccgcgcct ggtttgccgt
     9601 catcaccggc atgtcggccc cggggatggg tagcgccccg atcacggcgt tctggcgggc
     9661 gttgttgcgt actatctcct cacagacggc ccgcctgagc gggggatacc ccttcgcgag
     9721 cgcgaccaga aagtcctcgt ccagcgccag cacgatgcgt ggcgccagct cttcgagcat
     9781 ggcctccccg cttttcgaag ggagggagat ctcaccgggg tc
//
LOCUS       DATMVF010000266         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_315786, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000266 DATMVF010000000
VERSION     DATMVF010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315786"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VN178_13625"
     CDS             complement(<1..347)
                     /locus_tag="VN178_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82052.1"
                     /translation="MLSERREDLVAERTRALNRLHGLLRDLLPGGVARTLSADRAARI
                     LCSIRPKGTSARLRRRLASEILRDVRTLDRKVADLNERIEAEVDASGTTLTRIFGVGP
                     ILAARIIGAVGDVT"
     gene            complement(1066..1524)
                     /locus_tag="VN178_13630"
     CDS             complement(1066..1524)
                     /locus_tag="VN178_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82053.1"
                     /translation="MSDAADAPLFPPEEICAQAQSNAAAWILTMIAYLKERGGAVEDC
                     VAYHGRSFAPAWEGLRGSPVEEVAQLVALNALSAGSTLRSLSSEEGRAELLVEGWPNE
                     ELLGFLPLDREECDQIWDTYRPIMEHLGLGYAWQRQNDVVRIVVERQSGL"
     gene            1704..1907
                     /locus_tag="VN178_13635"
     CDS             1704..1907
                     /locus_tag="VN178_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82054.1"
                     /translation="MGHPHEDLQTLYAMKGRLEETGQMAKTLGLDNGGEAGTIYQALV
                     QVSSMVDNEIQSVRNIVGPHEDQ"
     gene            complement(2003..2347)
                     /locus_tag="VN178_13640"
     CDS             complement(2003..2347)
                     /locus_tag="VN178_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82055.1"
                     /translation="MVARAGGKGRLEPLYVRAGLSKVLLVFSFEEEAEMFLHLGGYAD
                     SGWRVRESSAGELVSVLCGPCIDVEGVALDPLPEMLEDGTIELVEVGRRRFVEQLLER
                     GANGLVVQARRF"
     gene            complement(2447..3199)
                     /locus_tag="VN178_13645"
     CDS             complement(2447..3199)
                     /locus_tag="VN178_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS82056.1"
                     /translation="MAERLEELLGVEEVAEYLGVGQVTVYRWCRAGSLPCLKIGRRWR
                     VRRDALAEFLRKSERSETLTGRLRAFLEVPDNVMAIAQDRDLMQRMDAAFFRVGEARG
                     GMLVKYHREAEGMPTAGELRSELSRNGLEVWRLEGEGRLRFVCERDEPGGRVREIERL
                     IAEGAGEGRSVWVDFNWESGLELEAALEQQRALSGVIEDTRIVIKTVVLEAKLDEWPG
                     STQRRAQVMHAGTMWLSESGLALSRVTPPPAL"
     gene            3354..3581
                     /locus_tag="VN178_13650"
     CDS             3354..3581
                     /locus_tag="VN178_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82057.1"
                     /translation="MTEASRQPKDKNYNLISVLYQSSDNAESLKTFVQDAEAEGDQEL
                     VEFFRGILENNLRAAQRAKEMLVPRFQSEHE"
BASE COUNT          739 a         1324 c         1085 g          683 t
ORIGIN      
        1 gtaacgtcgc cgaccgcccc gatgatcctg gccgccaata tgggacccac gccgaagatc
       61 cgggtgaggg tggtgccgga agcgtctacc tcggcttcga tgcgctcgtt gaggtcggct
      121 acctttctgt ccagcgtcct gacgtcgcgc aagatctccg aagccaagcg tcggcggagg
      181 cgggccgaag tacccttggg ccgtatggag cacaggatgc gcgccgctcg gtcggccgag
      241 agcgtccgtg ctaccccgcc ggggaggaga tcccgcagga gcccgtgcag gcggtttagc
      301 gcccgggtac gctctgcaac caggtcctct ctcctctcgg agagcaagcg cagcgcctcc
      361 gaggcgcctc gggatctacc gccgtcagcc atctgttgcg cgaggcggcc aacgccctag
      421 cgagagcatc tactccgtcg ttcttgcggg cgtttccggt agagagcacc cgcacccttg
      481 ccgagagctt gggctgctca cgtccaccac cgattcgcct gaacccgcca gtctcatcgc
      541 caggtgccta ccccgagccc gccggcgttt tccaccgccc agcgacggtc ggggaacctt
      601 ctcgcccatc gttcgaggcg cctcaagcct tctctaggtc ctgcgggaag ctggcacgct
      661 cgaggagctc gcctgtggct tcgtctaccg cagagcgacg gctaccgaac ctttgtgagg
      721 atcgaccccg atcaagatct ccatgagcgc acgcacctcc gtttcggtcg gagttctatg
      781 ccatcggcgt gcgcgaaggg cattccgact tcgggtctcg ctcccacacc tcatttgagc
      841 cacttcgccc gcctcgggca ccggcgggag gcaagccgag aacaagccaa cccgtcttgc
      901 gggcgacagg aagttcgcga gcgatccctg tataaccggt accctaggag cttagcggct
      961 ctagagacca ccgaactcct gctcggagtt aaacaagtcg gaagttcaag ttcgtgagta
     1021 acggttctca gaagttcata gcaagttacg acttatggct actagctaca gcccactctg
     1081 ccgttcgacg actatcctca ccacgtcgtt ctgacgctgc caagcgtagc cgaggcccag
     1141 gtgctccatg atgggcctgt aggtgtccca tatctggtcg cactcctcac gatcgagcgg
     1201 caagaagccc aagagttctt cgttcggcca gccctcgact agcagctcgg ctcgcccttc
     1261 ttcgctcgat agcgagcgta acgtactccc cgcagatagt gcgttcagcg ccaccagctg
     1321 cgcgacctct tcgaccggac taccgcgcaa tccttcccag gccggggcga agctgcgtcc
     1381 gtggtaggcc acgcagtcct cgaccgcccc cccacgctcc ttcaggtaag cgatcatggt
     1441 caggatccat gcggccgcgt tgctctgagc ttgggcgcag atctcttcgg gcgggaacag
     1501 cggtgcgtcg gcggcgtcgg acacgagggg cctccttact ctcctgtgta cgcatcagca
     1561 tgatgccagc cgaactagcc tggcgcacat gctcatcccc ttattcagcg gagtgcgtgg
     1621 aatagagatt ctccgaagta ttgcactagc gaggtgcagg gtacagttga atggaatata
     1681 gaccactgga aaaggaagag ccgatgggac acccacatga ggaccttcag actctgtacg
     1741 cgatgaaggg aaggctagaa gagaccggcc agatggctaa aactcttggc ctcgacaatg
     1801 gaggggaggc gggcaccatc taccaggctc tggtgcaggt tagctccatg gtcgataacg
     1861 agatccagtc cgtacgcaat attgtcggtc cccatgagga tcaatagcac ctcgtcacag
     1921 agcgcatgga agtggtgttc tccgaagtca acttccgctt tacgcagttc taagaagttg
     1981 acccagcggg cgttctccga agctaaaagc gccgggcttg caccacgagg ccgttcgctc
     2041 ccctctccaa tagttgctcc acaaagcgtc tgcgtcccac ctcgaccagt tcgatcgtcc
     2101 catcctcgag catctcggga agggggtcta gggccacgcc ctcgacgtcg atgcagggac
     2161 cgcagagcac cgagaccagt tcgcccgcgc tgctctccct cacccgccat ccgctgtcgg
     2221 cgtagccccc gaggtggagg aacatctctg cctcctcctc gaagctgaag accagcagaa
     2281 ccttcgaaag ccccgcgcgc acgtacaggg gctcgagccg gccttttccc ccggccctcg
     2341 ctaccatcca ccgcttggta ggagttggcc cgctcgggga cttcgtctcc caccgtggtg
     2401 cactttcggt acgcaaccct tgctccttcc tttccccgtg catccctcaa agggccgggg
     2461 gaggggtgac tcggctgagc gccagcccgg actccgacag ccacatcgtc cccgcgtgca
     2521 tcacctgcgc ccgcctctgt gtggaacccg gccactcgtc cagcttggcc tcgagcacta
     2581 ccgttttgat cacgatccgg gtatcctcga tgaccccgct cagcgcacgc tgttgctcca
     2641 gggccgcctc cagctcgagg cccgactccc agttgaaatc cacccacacc gaacgcccct
     2701 cgcccgcccc ctcggcgatc aaccgctcga tctcccggac gcgtccgccc ggctcgtccc
     2761 tctcgcagac gaagcggagc ctcccctcgc cctcgagccg ccacacctcc agcccgttgc
     2821 gcgaaagctc ggacctgagc tcgccggcgg tgggcatccc ttcggcctcg cggtggtact
     2881 tcacgagcat cccgcccctg gcctcgccca cccggaagaa ggcggcgtcc atccgttgca
     2941 tgaggtcacg gtcctgggct atggccatca cattgtcggg gacctccagg aaggccctca
     3001 ggcgccccgt gagggtctca gagcgctcgc tcttcctcaa gaactccgcc aaagcgtcgc
     3061 gcctgacgcg ccagcggcgc ccaatcttca ggcacggcag cgagcctgcc ctgcaccagc
     3121 ggtagaccgt cacctgcccc acgccgaggt attctgcgac ctcctcgacg ccgagcaact
     3181 cttccaaccg ctccgccacg ctcctcaccc ctctcggtcc cgagcattgc cttcgcggcc
     3241 gcgccgagcc cggagactta cgactttata gtacttgatc gtacttaagt ttacactacg
     3301 gaacctcggg gtactctagg aatcgcaagc agatcagaca gaaaggagaa agggtgaccg
     3361 aagcgtccag gcagcccaag gacaagaact acaacctcat cagcgtgctc taccagtctt
     3421 cggacaacgc cgagtcgctg aagaccttcg tccaagacgc ggaggccgag ggcgaccagg
     3481 agctggtgga gttctttagg gggatcctag agaacaacct tcgcgcggcc caaagggcca
     3541 aggagatgct cgtcccgcgc ttccagagcg agcacgagta gaccgggact aggcttacgg
     3601 gctcaggcct ttatcgatct ttcgcgacaa acactaccgg aacggcatcg agacgccgat
     3661 ccgcccagga ggaggatata gctatggccg acgacaagct ccaacagaag ctgaccgact
     3721 acgtcgagga cgcccacgcc atggagcaga acgacctcaa gatgatcgac tccatgatct
     3781 ccaccaccga cgacccccag accaaagaga tgctgcaaga gcacaagcgc g
//
LOCUS       DATMVF010000267        20926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_679247, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000267 DATMVF010000000
VERSION     DATMVF010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 20926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20926
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679247"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1105)
                     /locus_tag="VN178_13655"
     CDS             complement(200..1105)
                     /locus_tag="VN178_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWS82058.1"
                     /translation="MGGSLLRRIPPARPYLVATVVVGLLVTVVTVAQMAFLSELVDRV
                     FLNGGGRGQVLTLLSLLLGAGVLRAGLLWVRETTAQQGAVQVKNDLRERLFAHVLQLG
                     PAYARSERSGELTTTLTEGVERLDPYFARYLPQVFLSVSVPLLVVCYILPHDIASAVL
                     LVVTAPVIPVMMLLVGSYAEEHTRRQWFAISRMGAHFLDALQGLPTLKAFGRVAAEGE
                     RVERVSGEFRERTMKVLRYAFLSGLVLEFMTAAAIALVAVVLGVRLINGAISFQDAFL
                     VLLLTPEFYRPPARGRHPPPRRDGG"
     gene            complement(1106..1261)
                     /locus_tag="VN178_13660"
     CDS             complement(1106..1261)
                     /locus_tag="VN178_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82059.1"
                     /translation="MLREARSARVMLLAWRAGCGGHWLETGGGERVAEMRSGMTVRGF
                     FPERTGN"
     gene            1491..1856
                     /locus_tag="VN178_13665"
     CDS             1491..1856
                     /locus_tag="VN178_13665"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS82060.1"
                     /translation="MSARRAKPGSLVENAVDLQEVAAGRGVVWTLQASEDLNANLARF
                     AKGGGVGEHVNEEVDVLLVGVAGSGSVVVDGHEHALGAGKLVFVPKGSRRSTRGASEG
                     FAYLTVHRRRGPLRIGSGR"
     gene            complement(2097..2384)
                     /locus_tag="VN178_13670"
     CDS             complement(2097..2384)
                     /locus_tag="VN178_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS82061.1"
                     /translation="MRFASARKEQEYEARRSLESLTDREKEVLSLMVEGLDAGEISER
                     LYISAKTERNHAASILSKLGVHSRLQAVIFAARHGAVEVGGGTTARAGRRS"
     gene            2389..3024
                     /locus_tag="VN178_13675"
     CDS             2389..3024
                     /locus_tag="VN178_13675"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWS82062.1"
                     /translation="MECLLSARTADAVFVVDPEQRIVHWDPNAETLTCLLAEEMVGRP
                     CHQALRGECEGGGRLCKGGCPVMALARAGHAVPSYDMRVETRIAGKRWVSVSVLSVDS
                     DDGPYLVHLLRDAQKAHDALEMARDLVRLTSKGENASTTYHEVVPELTPRQAEVLGLL
                     AEGRSVKEICAELCLSKATVRNHVRALLQALGAHSQLEAVAAARKLGLLRG"
     gene            complement(3028..3765)
                     /locus_tag="VN178_13680"
     CDS             complement(3028..3765)
                     /locus_tag="VN178_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWS82063.1"
                     /translation="MSEAVLVFLLASLTAVATGLGALPFLFAQEPTRKWLGISNAAAA
                     GLMLAASLGLAYEGALRGTLGVAVGAAVGLAFIVLVRRFLSADEHPAVFAGMGGLDAR
                     KGLLIVGVMTAHSFTEGVGLGVSFGGGADLGLFITIAIAVHNVPEGLAISLVLVPRGV
                     GVGLAAFWSVFSSLPQPLMALPAFHFVEVFRPLLPYGLGFAAGAMAWMVFAELLPDAR
                     KEAPARLVVAVVALSVLAMLAFQVLVR"
     gene            4319..4705
                     /locus_tag="VN178_13685"
     CDS             4319..4705
                     /locus_tag="VN178_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82064.1"
                     /translation="MAKRRGSVIERVLKRIEDSVEEYRAKERVREVDLQAHRAELWAE
                     AAERQRLLAEALAEGEADRASLEEITKEERVVFVLHREDLTATLEDFAGKKDCLVNVV
                     PGKGDYGGGRGIDGLRGSWLVFEASR"
     gene            4702..5025
                     /locus_tag="VN178_13690"
     CDS             4702..5025
                     /locus_tag="VN178_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82065.1"
                     /translation="MKEAAWFAGSGKKMEADQVRAFEAFSGRFGDRFRWHERSEDNVV
                     ASILPLHADDVRWASQIATRCALPIVPVGAGTAPDDGSGTRRNAVQIRFDLMSGTNLS
                     KDDEE"
     gene            5022..5666
                     /locus_tag="VN178_13695"
     CDS             5022..5666
                     /locus_tag="VN178_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWS82066.1"
                     /translation="MIEAEPGASWLQLDNELRRRGRGLAVYPTSAPRATVGGWLATDG
                     IGVGSFEFGRLYENVISATVVGRGGEIREIGSDELGRAFMPMKAGSVVVGARLRTRRA
                     ATDRVFAAAFEDARSLAGAVGDLASSETPLWHLAFVNPAMAAARGLRSRYLLFGAYPD
                     HRDGEGWWELRRRVLQARGATELVVPETWLIWGRGSSRWRPAKGPRMQRASSSR"
     gene            complement(6082..6702)
                     /locus_tag="VN178_13700"
     CDS             complement(6082..6702)
                     /locus_tag="VN178_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82067.1"
                     /translation="MAIHPEVFLAAGYAIFLVCVSLGLDALARHSHARSERYRTAGFT
                     YHHPSDAWICPEDQLLLRSEVDHGRRLIRYRGRPHICNSCPTKPECTDSDEGREVVHA
                     MDPWLHSEAGRFHRGISLAVVLIAGLILAAETLRNHAPLELAVLGVVFLAVVLTGWHL
                     HAVFRNSPANFPVPKVPPIPPVQPLRAGYRKAKSEGADPRREVPRR"
     gene            complement(6706..9009)
                     /locus_tag="VN178_13705"
     CDS             complement(6706..9009)
                     /locus_tag="VN178_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWS82068.1"
                     /translation="MVSGSGRRELLVSKGWVQAVALVVLFGFFVLGLLAYRAYSGQPP
                     IPERVVDSSGRVIFTGEDVTAGQGVFLRNGLMQYGSVFGHGAYLGPDYTADYLRRSAL
                     SVRESYGGEASDEAAARTVEDFKENRYDPESRTLEFTAAQAAAFGELRGYYGDYFGEP
                     TTRYGLRPEAIEDPGDIRQLTAYFAWTAWAASTLRPGLDYTYTNNWPPEPLVENEPTG
                     EVVVWSVLSLVALLGGIGLLFAAFGRWNFLGWRGREQATLSFRTPGDVALTPAQRACA
                     WFFLVMAALFLIQTLVGAASEHYRADLAGFFGIDLAQVLPYNLMRTWHVQLAIFWVAT
                     SFLAAGIFLAPMIAGREPRGQHLLAYGLLGALAVVVFGSMIGEWLGIFGLMGGWWAWF
                     GNQGFEYLDLGRFWQVLLSIGLFFWVAVLFRGLRGRLRREHAANMPWLFFFAALAIPA
                     FYAVGLLARPESHFTTADFWRFWVVHLWVEDFLELFTTVMVAYIFVLLGVVREKVALT
                     VIFLDILLYSAGGVIGTMHHLYFSGTPAEHMALGAFFSAAEVIPLTFLTVEAWSFLQL
                     GARQESRSRTPFPHRWAVMFLVAVGFWNFLGAGIFGFLINLPIISYYQIGTALTANHG
                     HAAMMGVYGMLAAGLALFCLRYLIPEDRWSERAAKISFWSLNIGLAWMCFATLFPLGI
                     LQLYESVNSGYFEARSLEYITNDTNALLEWMRLPGDVVFIVGGVLPLLYLCYLGVRHT
                     VPRVTMEEPEDILFTDIAEPETAGQRE"
     gene            complement(9133..9564)
                     /locus_tag="VN178_13710"
     CDS             complement(9133..9564)
                     /locus_tag="VN178_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWS82069.1"
                     /translation="MRLELSSEGRYALRALVYLAEIGEQASANRISAEANVPRRLLAR
                     ILARLSHAGLVESEQGRRGGSRLARPPQEISLRNAVEAAEGPFGVTRCIMQNRACGSG
                     PPCAMHNAWEEGQQAILGYLETQTLDDFVQETASRRLTPGP"
     gene            9733..9933
                     /locus_tag="VN178_13715"
     CDS             9733..9933
                     /locus_tag="VN178_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82070.1"
                     /translation="MNASFFFAGTAGANPLMFILAILLMLSWRVAGYRGLDRWVLPAI
                     GIPGYPGTLFSGRAEGQRSRVK"
     gene            complement(10009..10212)
                     /locus_tag="VN178_13720"
     CDS             complement(10009..10212)
                     /locus_tag="VN178_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82071.1"
                     /translation="MVWWSDLLWGFWNGLTAWIVLVVHVFGGWEQFPFYNAERGGNWY
                     DFGFLVGAGSPLLGVVGGRGRRS"
     gene            10421..11518
                     /locus_tag="VN178_13725"
     CDS             10421..11518
                     /locus_tag="VN178_13725"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HWS82072.1"
                     /translation="MAVGDYEAIFVGGGLAAMLLLREIGPGLSGRVAVIDPCPPSERP
                     TIHWSYWGHVPTLYDRFAVGSWRMARVAQMPAESVAPYTLRLVRSTDVFAQIAEELST
                     VSLEWLRTSAHSVERREDGTYGVITDAGAVRAGWVFDSVPDIEPAFPSPRRPRAVLSG
                     TGVRVTVDRPVFDAAAATLFDPLDERSFAYLLPLSPDEALLESATFDSAIQKKDETPL
                     LRYLRSRYPGATFAVTHAEHGHIPLGFAPSQTAGPRHVLLGTKRGLVKPSAGYGVVRM
                     ALESRRLAGLWKQGRPLPPSQRSSLWWQLLDLGFLQLAANDSRRPLALLRNVMHAVPL
                     VQSLRFIDEELPPRQLAAVFGAALPAVLGRV"
     gene            complement(11604..13034)
                     /locus_tag="VN178_13730"
     CDS             complement(11604..13034)
                     /locus_tag="VN178_13730"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWS82073.1"
                     /translation="MTDERPRSESSVLGGLLSGSTLKRRLLSGSIWAVAGRFGGSLIS
                     ILTASLLTFVISPKEVGAYTLVLTIVSFGALVGALGLPKTVVRLVAENMGLNRSGRTR
                     RVIYTVLGLGVAGTLLTSLAYYLVIGDIVEGRLFDSPLLLGLTGLIAVWMAIAIVQEI
                     TAETFRGFHDLRWATLLGGLATGGKSGGLIMKLLLLGALALLVVTGASVDLRTVTLVS
                     IGAGSVSVLLSCWLLYGRVSSLGSKATEEEPVSAREVLDDAIPFLGIAMTSFVLLTAD
                     IWILGAFDSETSVAVYSNASRLVTFVVMPLLVVNLVMPPIVAEMYAQGRIARLERTIR
                     AFSTLAGVPALLVLVGFMLLGGPILGLLFNQDIYSSNTAWLVLLILSAAKLVAVWSGS
                     CGLVLQFTGHQGSMLRVSLLTSPLFFVLAIFAVTRYGPVGVAAAAAITTSLQNVIMVL
                     LAKRKTGMWTHVSFSLAPFRKALKKD"
     gene            complement(13244..13468)
                     /locus_tag="VN178_13735"
     CDS             complement(13244..13468)
                     /locus_tag="VN178_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82074.1"
                     /translation="MGDLGRTAAREMLSSQRGIDSFSQRLPMAQQQWRLPFQGGLGYT
                     FDGMDADLVAFILPSAAAVFSQFRRSPRAT"
     gene            complement(13523..13870)
                     /locus_tag="VN178_13740"
     CDS             complement(13523..13870)
                     /locus_tag="VN178_13740"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWS82075.1"
                     /translation="MSELSEMKCTACRGDEPTLTDAEIAQMHPQVPGWQVVQREGIKR
                     LERVFRFRNFAQALAFTNRVGEQAEAEGHHPALLTEWGRVTVTWWTHKIGGLHQNDFI
                     MAARTDELSEGRT"
     gene            complement(13944..14723)
                     /locus_tag="VN178_13745"
     CDS             complement(13944..14723)
                     /locus_tag="VN178_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWS82076.1"
                     /translation="MRLGEHSWNEIEGLTERVAVLPLGSFEQHGHHLPLLTDTMICTE
                     IVRRAEVELGDEALFLPTLWVGASDHHRAFPGTVSIRNETYVRVLTDALESLIDAGFR
                     RVFLLNAHGGNVTPGRQAIYDVQLKHREETDLWLAFASWMTVAAERIAALEEVEQEHV
                     SHACEQETSMILSLRPELVKPGAAHGANIPFESAFYCPDFSRPSRVDVARTFDQLSET
                     GAFGHPETGTAEKGEALFEAAAGEVVSFVREFAGWPGIRPH"
     gene            complement(15022..15174)
                     /locus_tag="VN178_13750"
     CDS             complement(15022..15174)
                     /locus_tag="VN178_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82077.1"
                     /translation="MRSEHLPQRFDGCQDKHRGTMGTLRCEKQAWSFTDEVEQIGGEL
                     CGDFRL"
     gene            15441..16625
                     /locus_tag="VN178_13755"
     CDS             15441..16625
                     /locus_tag="VN178_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS82078.1"
                     /translation="MTGTRETAALERAATQLQVDGFEKQHPNIELQRETLQPDEVRRE
                     IESRLRSKEPPDVFAYDTGPGFGGVLADAGLLRSLENAYTKYGWDIYDWAKQQATYEG
                     TVYGVPDQVEEIIVYYNKDLMPEVPRTVEELREISGELMGRRKIPLAFGNRERYPASH
                     MFSIATSNVLGREGLDSILYGDGRWDIPQVVEAIELMFRDFVESGYYPKSPNALTYDG
                     ANALFYSGEAAMNPTGTWLVSEIVHAVQEFEVGFFPFPSIEGSGISPPAGVGTGWFVA
                     NGAGNPQEAFTFIDYLLQDDTARLTIELLNTIPAHPVDTEGLDVPELFKQVLDDLSEY
                     PRAKAFGYNIDVLTPQNFNEVMYTGFREVMNGMRSPAEQAVALPRAWTKAKEVGKTPA
                     QE"
     gene            16830..17183
                     /locus_tag="VN178_13760"
     CDS             16830..17183
                     /locus_tag="VN178_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82079.1"
                     /translation="MFARVSTYEGRPEQLDEMHHEGVEHILPALEMQDGYSGGLVLAD
                     RQSGKVLALTLWESEQAMDATEEASQWLRIFSAESSGGTISSVERYEVFYAAIQEAPP
                     SERSGEPGSTETRRP"
     gene            complement(17223..19865)
                     /locus_tag="VN178_13765"
     CDS             complement(17223..19865)
                     /locus_tag="VN178_13765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWS82080.1"
                     /translation="MTLTVLGLFLLVVSRSPVGVPVYAGAPVFDYWLENTIIAVSFST
                     VGAIITPRLPPQNPIGWIFCSIGVIAGMRLFVSEYAIVTLLAEPRSVPAMLPGGEVLA
                     WISSWLWVSHIGLFVFLSVLFPDGRVPSSRWRPFGWLVGAVIVIGTVSVALWPESAAG
                     FDLVNHPLGIEVATETVNPVETILYALGLIAAASLLVRLRRSMGVQRQQVKWFAYAVS
                     VLATSAIFAYVVSESVGVVWLGWVSSMLVIASVVGLPIAVGIAILRYRLYNIDLLLNR
                     TLVYGALTAVLATVYFGSIVLFQMVFRAFTGQESQLAVVISTLAIAALFTPLRLRIQS
                     FIDRRFYRRKYDARKTLEAFSAKLRIETDLDALSSELIDVIRETMQPTRVSLWLKAQE
                     RAREATKLATTAIEEPEIEIAPDDPILAYLSNVSGVVEVEKLDLDSPALGAMKSAQIE
                     LVVPLVSQGELIGLLSLGPRLSQQEYSADDRKLLKDLSTQTAPAVRVARLVRQQQEAE
                     TRYRTLVEQTPAITYVQEALESSNPKAVTYVSPQYETILGFSPGSQMIDEEHWLRAVH
                     PEDRERVLAEEARTDQTGEHFNVEYRVVAGDGRVVWVRDEATLVCDEEGQPLYWLGIQ
                     YDVTEQKREAKERERIEQELRIARLIQQTLLPKTLPKLSAYDIAAYYKPAREVGGDFY
                     DSFVLEDGRLGLVVGDVTDKGIPAALVMATTRTMLRVSAQRHFPPGEVLKRANDALVP
                     DIPPNMFITCLYAILDTESGRLVYANAGHDPPYLRHNGSDVEELRARGMPLGLMPGME
                     YEEKEVTLQRGESVLFYSDGLVEAHDPKGEMFGFPRLQGLVGAHHSGESSLIDFLLSE
                     LTDFTGENWEQEDDITLVTLERSD"
     gene            complement(20560..>20926)
                     /locus_tag="VN178_13770"
     CDS             complement(20560..>20926)
                     /locus_tag="VN178_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82081.1"
                     /translation="EISGGAQGRATYEWMEGGFFLIQRVDLQQHGQKIKGIEIIGHER
                     EFGAEPSEDIGSRFYDNMGNTLDYVYEIEGDTLTIWGGERGSPAYYRGTFSEDDKTIS
                     GAWHYPGGGGYEATSTRVE"
BASE COUNT         4014 a         6749 c         6532 g         3631 t
ORIGIN      
        1 gctgcgcccc accacggccg tgcgggagcc ggccggcagg gtgagggtga gcccgtcgag
       61 cgcagggacg tctcgccccg gataggtgaa acggacaccg gagaacgaga tctccaatcc
      121 cccagccagg ctcacctgcg aacttccgtc cgcgggcgcc ggggtatcta gtatctcgaa
      181 gatccgttcg gcggcggctt taccctccat cccggcgtgg cggtgggtgc cgacctcgcg
      241 caggggggcg gtagaactcc ggtgtcagga ggagcaccag gaaggcgtcc tggaacgaga
      301 tggcgccgtt gatcaggcgc acccccagga ccaccgcaac gagcgctatg gcggcggccg
      361 tcataaactc gagcaccagc ccggagagga acgcgtagcg cagcactttc atcgttcgct
      421 ccctgaactc accgctaacc cgctccactc tctctccctc ggccgcgacg cgtccgaacg
      481 cttttagcgt cggcaggccc tgcagggcgt cgaggaagtg cgcacccata cgcgagatcg
      541 cgaaccactg gcgccgcgtg tgctcctcgg cgtagctccc gaccaggagc atcatcaccg
      601 gtatgaccgg cgctgtaacg accagcagga ctgcgctggc gatgtcgtgg gggaggatgt
      661 agcacactac cagcagggga acagagacac taaggaagac ctgcggcagg tagcgggcga
      721 agtaaggatc caacctctct acgccctccg tcagggtcgt cgtgagctcg ccggaacgct
      781 cgctgcgggc gtaggccggt cccagctgca gcacgtgggc gaaaagcctc tccctgaggt
      841 cgttctttac ctgaaccgca ccttgctgcg ccgtcgtctc cctgacccag agcaaccctg
      901 cccgcagaac cccggctccc aggagcagcg aaagcagggt gagaacctgc ccgcggcctc
      961 ctccgttcag aaagacccgg tccactaact cgctcagaaa ggccatctgg gcgaccgtta
     1021 cgacggtcac caggagcccg accacgaccg ttgccaccag atacggccgg gcaggcggga
     1081 tccgccggag caggcttcca cccattcagt tacccgtccg ctccgggaag aacccccgca
     1141 cagtcatgcc actcctcatc tccgcaacac gctcgcctcc tccggtctcc aaccagtgcc
     1201 cgccacaccc ggccctccat gcgagcagca taacgcgggc ggagcgcgct tccctgagca
     1261 attttcccta atctcctgat cccgatagac cagtcgccct caaagcgtac cgcccctcgc
     1321 tgctcgactc caggtgcaaa cactttactc ccccacagac cttcgaacct ctcctatttt
     1381 cgctcaccca ctcgtccgaa gatacgcgag aggccatcgc ctcctctatt acacaggaag
     1441 cattagaatt cgtgcggcgt tggttcggtg gacggaggag gcgggactgg atgtcagcac
     1501 gaagagcgaa acccggtagc ttggtcgaga acgctgtgga tctccaagag gtcgcagccg
     1561 gccgcggcgt cgtgtggacc ttacaagcca gcgaggacct caatgccaac ctggcacgtt
     1621 tcgcgaaagg cgggggcgtc ggggagcacg tcaacgaaga ggtagacgtg ctcctggtcg
     1681 gcgtcgccgg gtcagggagt gtcgtcgtgg acggccacga gcacgccctg ggcgccggga
     1741 agctagtctt cgtccccaaa ggatcccggc gctcgacccg aggcgcctcg gagggcttcg
     1801 cctacctcac cgtccacagg agacgcgggc cgcttcggat agggtcgggg aggtagccaa
     1861 acaagcctcg gggtgttgcg cgggctcgag gatgggaagg aggaagcatg aagcgcgccg
     1921 cggagtcgaa gagcctttcg gaggaccacc atcacggcct ggcacacccc cggcggctcc
     1981 ggctggcggc cttgggaccg ggcgacagcg agggaggcgg ctgtgacagc gttcgatgga
     2041 cttaccgcag aggcggtttg ccttcgcgcc gtgaccaagt tggaccatct cggtggtcag
     2101 gatcgtctac ccgcccgcgc cgttgtgccc ccgcccactt ccaccgcgcc gtggcgcgcg
     2161 gcgaagatga ccgcctgcaa gcgggagtgg acgcccagct tggagaggat gctcgccgcg
     2221 tggttgcgct ccgtcttggc gctgatgtag agccgttcgg agatctcgcc tgcgtccaga
     2281 ccctcgacca tgagcgagag cacctccttc tccctgtcgg tgaggctctc cagcgaccgt
     2341 ctggcctcgt actcctgctc cttgcgggcg ctcgcgaagc gcaccagcgt ggagtgcctg
     2401 ctaagtgccc gcacggcgga cgccgtcttc gtggtggacc cggaacagcg gatcgtccac
     2461 tgggacccga acgcggagac gctgacgtgt ctcctggccg aggagatggt cggcaggccc
     2521 tgccaccagg cgctgcgcgg cgagtgcgag ggcggcggcc ggttgtgtaa gggaggatgc
     2581 cctgtcatgg cgctcgcccg ggccggccac gcggtcccca gctacgacat gcgcgtagag
     2641 acccgcatcg ccggcaaacg ctgggtcagc gtgagcgtac tgagcgtgga ctccgacgac
     2701 ggaccctacc tggtccacct gctgcgcgac gcccagaagg cccacgacgc cctggagatg
     2761 gcccgcgacc tggtccggct cacttcgaag ggagagaacg cctccaccac gtaccacgaa
     2821 gtagttccgg agctcacgcc gaggcaggcg gaggtcttgg gacttctcgc cgagggcagg
     2881 tcggtcaagg agatctgcgc cgagctctgc ctctccaagg ccaccgtgcg caaccacgtt
     2941 cgcgcgctgt tgcaggctct gggcgcccat tcccagctcg aagccgtcgc cgcggcccgc
     3001 aagctaggtc tcctgagagg ttagcggtca cctcacgagg acctggaacg ccagcatggc
     3061 gaggacggag agcgcgacga ccgcgacgac gagacgggcc ggagcctcct ttctagcgtc
     3121 tggcagaagc tcggcgaaga ccatccacgc catcgctccc gccgcgaacc caagcccgta
     3181 gggcagtagc ggccggaaca cctcgacgaa gtggaaggcg ggcagcgcca taagcggctg
     3241 cggtaggctg gagaagacgc tccagaacgc cgccaggcca acgccaaccc cgcgcgggac
     3301 gagcacgaga cttatggcga gcccctcggg gacgttgtgg acggcgatgg cgatcgtgat
     3361 gaacaggccc aggtccgccc cgccgccgaa ggagacgccg aggccgacgc cctcggtgaa
     3421 cgagtgggcg gtcatgacgc ccacgatcag gagccccttt cgggcgtcga gaccgcccat
     3481 ccccgcgaat accgcagggt gctcgtctgc gctcaggaac cggcgtacca gcacgatgaa
     3541 ggcaagcccg accgcggccc ctaccgccac tcccagggtt ccccgcaacg cgccctcgta
     3601 ggcgagcccc aggctcgccg ccagcatgag ccctgcagcg gccgcgttgg agatgccgag
     3661 ccacttccgg gtcggctctt gggcgaagag gaagggcagc gcgccgaggc ccgtcgccac
     3721 cgcggtcagc gacgccagga ggaagaccag tacggcttcg ctcattgcga cgccgacccg
     3781 tccgtcgggc tcatgtctcg ctgcgtggcg gtgggcagac gaggaccggg cctccggtcg
     3841 tatgcaactc cgagctgtcc atcgccagaa gtaccttcct cgggaacatc atcgcacttc
     3901 ctccttgacc tcgtctcctc gggtactacc tggccaccct ccagccccga cagcacgtag
     3961 ggcgtccagg cccgcaagac gaccacgaac ccgcggtgtt cgccatagtg tgctcctctc
     4021 gctctcgtcc ctgtcgcaaa ccatcatagt cgcggatggc tcgcgttaac tggtcaggac
     4081 tgcccattcc attagccaag accgaccaat cgctggcgtt gtcgcccctt ggtcgtttcg
     4141 agatgatcaa tctgtgcgag atcttgggcc atctggctca ggagcatcgt cctctcgcac
     4201 cgtagagtcc cctcatggca gatggaccac gacagcgttc gagggagaag gggcgcaggc
     4261 tatccgacga attcgcgagg gcgccgtggt cgaaatccgg aagacgagga ggtgcgcgat
     4321 ggccaaacgg cgagggtccg tgatcgagag ggtcctgaag agaatcgagg acagcgtgga
     4381 ggagtaccgc gccaaggaga gggtccgcga ggtagatttg caggcacaca gggcagagct
     4441 ttgggccgag gccgccgagc gccagcggtt gctggcagag gcactggccg agggagaggc
     4501 cgaccgggct tccctggagg agataaccaa ggaggagcgt gtggtcttcg ttctgcaccg
     4561 cgaggacctg acggccacgc tggaggactt cgccggcaag aaggactgcc tggtgaacgt
     4621 ggtgccaggc aagggggact acggtggtgg caggggcatc gacggcctga ggggttcgtg
     4681 gctggtgttc gaggcgtcgc gatgaaggaa gctgcatggt tcgccggtag tggcaagaag
     4741 atggaagcag accaggtaag ggccttcgag gctttttcag ggcgcttcgg ggaccgattc
     4801 aggtggcacg agcggtcgga agacaatgtt gtggcatcta tcctccccct gcatgcggac
     4861 gacgtgcggt gggcctccca gatcgccacg cgatgcgcgc tgccgatcgt gcccgtcgga
     4921 gctggcaccg cgcctgacga cggctccggg acgaggagga acgccgtcca gatccgcttc
     4981 gacctgatga gcggcacgaa cctttccaag gatgacgaag aatgatagag gcggagccgg
     5041 gggcgtcctg gctccagctg gacaacgagt tgcgccgacg ggggaggggc ctcgccgtct
     5101 accccacgag cgccccccgt gccaccgtgg gcggctggct tgccaccgac ggcatcggcg
     5161 tcggctcctt cgagttcggc aggctctacg agaacgttat ctccgccacc gtggtcgggc
     5221 gcggcggcga gatccgcgag atcggaagcg acgagttggg ccgcgctttc atgccgatga
     5281 aggccggcag cgtcgtcgtc ggagcaaggc taaggacccg gcgcgcggcc acggatcgtg
     5341 tcttcgcagc agccttcgag gacgcgcgaa gtctcgccgg ggcggtgggg gatctggcct
     5401 cttccgaaac accgttgtgg cacctggcgt tcgtcaaccc cgccatggca gcggcccgtg
     5461 ggttgcgctc acgttacctg ctcttcggcg cctaccctga tcacagggac ggagaggggt
     5521 ggtgggagct acgccggcgg gtgctccagg cccgcggcgc gaccgagctt gtcgtcccgg
     5581 agacctggct gatctggggg agaggttctt cccggtggcg cccggccaaa ggaccccgga
     5641 tgcagcgcgc gagttcgtcc cgctagcgga tctcccacgg atgctcgagg acaccaaccc
     5701 taccgatgcc atgcaaggca ctgtctcgcg ctccggcgag acgctgctcc tgaccctgag
     5761 cgacggcggc gatacggttg gtccaggatg accacgccat tggagcgaat tgcagaagaa
     5821 ggccgtgcgg ctctctgccc cgcgacatag tcggcggcct accgagggcc ggagagggta
     5881 atgtagggcc gccggcacaa ctggatgctg gagaaccccg agtcattcgc tccgatggtc
     5941 gagacctgga taaatgacga gctgcctccc acgtcgctcc ggacccgacg gagtttcggg
     6001 ccggcgatct gccccggagc ctagcgagcg cggggcggca cggcctgaac ggtcttgccc
     6061 actctctctg gccaacattg atcatctgcg cgggacctct cgtctgggat cggcgccttc
     6121 actcttcgct ttcctgtagc cggcgcgaag aggttggacg ggagggatcg gagggacctt
     6181 cgggaccgga aagtttgccg ggctgttgcg gaacaccgcg tgcaggtgcc atcccgtcag
     6241 cacgaccgcc aggaatacga cgccgagcac tgccagctcg agtggtgcgt ggttgcggag
     6301 ggtctcggcc gcaaggatca ggccagcgat caggaccacc gcgagcgaga tgccccggtg
     6361 gaagcggccc gcctccgagt gcagccaggg atccatcgcg tgcaccacct ccctgccctc
     6421 gtcggagtcg gtgcactcgg gcttggtggg gcagctgttg cagatgtggg gccgtccccg
     6481 gtagcgtatc aggcgccttc cgtgatccac ctcgctcctc aggaggagct ggtcctcggg
     6541 acagatccag gcgtcgctcg ggtgatggta ggtgaacccg gcggtgcggt agcgctcgga
     6601 gcgggcgtgc gagtggcggg ccagagcgtc gaggcccaaa gagacgcaga ccaggaagat
     6661 ggcgtacccc gctgcgagaa acacctccgg atgtatggcc atgatctact cccgctgccc
     6721 ggccgtctcg ggctcggcga tgtcggtgaa caggatgtcc tccggctcct ccatcgtcac
     6781 tcgcgggacg gtatggcgca cgcccaggta acacaggtac agcagcggca gcactccgcc
     6841 gacgataaac accacgtcgc ccggaagcct catccattcc aggagggcgt ttgtgtcgtt
     6901 ggtgatgtac tccaggctgc gggcctcgaa gtacccggag ttgacggact cgtagagctg
     6961 gaggatgcca agggggaaga gcgtagcgaa gcacatccag gcgagcccga tgttcagaga
     7021 ccagaagcta atctttgccg ccctctccga ccagcggtct tcggggatca ggtagcgtaa
     7081 acagaacagg gccaggccag cggcgagcat cccgtagacg cccatcatgg ccgcgtggcc
     7141 gtggttggcg gtcagggcgg tgccgatctg gtagtaagaa attatgggga ggttgatcag
     7201 gaacccgaag atcccagctc ccaggaagtt ccagaacccg accgccacca ggaacatgac
     7261 cgcccagcgg tgcgggaacg gcgtgcgcga gcgcgactcc tgcctggccc cgagctgcaa
     7321 gaaactccac gcctccaccg tcaggaacgt cagggggatg acctccgccg ccgagaagaa
     7381 cgcgccgagg gccatgtgct cggcgggcgt gcccgagaaa tacaggtggt gcatggtccc
     7441 gatcacaccg cccgccgagt acagcaatat gtccaggaag atgacggtca gggccacctt
     7501 ctcccttacc accccgagca ggacgaagat gtaggcgacc atgactgtgg tgaagagttc
     7561 cagaaaatcc tccacccaca ggtgcacgac ccagaaccgc cagaagtccg cggtcgtgaa
     7621 atgagactcg ggcctggcga gcaggccgac ggcgtagaag gccgggatgg ccagggcggc
     7681 gaagaagaac agccagggca tgttcgccgc gtgctcccgc cgcagccgtc cgcgcaggcc
     7741 cctgaaaagc acggccaccc agaagaagag gccgatagag agcaggacct gccagaagcg
     7801 tcccaaatcc aggtactcga acccctggtt gccgaaccag gcccaccatc cgcccatgag
     7861 cccgaagatg ccgagccact ccccgatcat gctcccgaac accaccaccg caagggcccc
     7921 gagcagcccg taagccagca agtgctggcc ccgcggctcc ctgccggcga tcatcggcgc
     7981 gaggaagatg cctgctgcca gaaacgacgt tgcaacccag aaaatggcca actgcacgtg
     8041 ccaggtacgc atgaggttgt aaggcaaaac ctgcgcaagg tctattccga agaatcccgc
     8101 cagatccgcc cggtaatgct ccgaagccgc cccgacgagc gtctggatca agaacaacgc
     8161 ggccatcacc aggaagaacc aggcgcacgc ccgctgcgcc ggcgtcaggg caacgtcacc
     8221 cggcgtccgg aacgagagcg tagcctgctc gcgcccccgc cacccaagga agttccaccg
     8281 tccgaaggcg gcgaagagca acccgatgcc cccgagcagc gcgaccaagg acaatacgct
     8341 ccacaccacc acctcgcccg tgggctcgtt ctccacgagc ggctccggcg gccagttgtt
     8401 ggtgtacgtg tagtcgagtc ctggtctcaa ggtcgaagcc gcccacgccg tccaggcgaa
     8461 gtacgccgtg agttgccgga tatccccggg atcctcgatc gcctccggcc tcagcccgta
     8521 cctggtggtc ggctctccga agtagtcccc gtaatatccg cgcaactccc cgaaggccgc
     8581 cgcctgcgcc gcggtaaact cgagcgtccg actttcggga tcgtagcggt tctccttgaa
     8641 atcctcgacg gtgcgcgcgg ccgcctcgtc ggaagcctct cccccgtagc tctcgcgcac
     8701 ggagagcgcg gagcgccgca ggtagtcggc cgtgtaatcc ggcccgagat aggccccatg
     8761 cccgaagacg gatccgtact gcatcagtcc gttcctcagg aagacaccct gcccggccgt
     8821 cacgtcctcg ccggtaaaga tcaccctccc gctagagtcc actacccgct cagggatcgg
     8881 cggctgaccc gaatacgccc ggtacgcgag aagaccgagc acgaaaaagc cgaacagaac
     8941 gaccagcgca acggcctgga cccacccctt cgatactagc aactcccgcc gcccagaccc
     9001 tgataccatc ggctacctcc agacggtcgc ggccggaaac tcctcccggt caagaatagt
     9061 tacacaatag gtaatataaa ggggggttgg cgtggtgcaa gggctgtggc gacgggcgtc
     9121 cgttcccagt cttcagggtc ctggggtcag tcttcttgag gcggtctcct ggacgaagtc
     9181 gtcgagggtc tgggtttcca ggtatccgag gatggcttgc tgtccttctt cccaggcgtt
     9241 gtgcatggcg catggaggac cgctgccgca ggcgcggttc tgcatgatgc agcgggtaac
     9301 gccgaagggc ccttcggctg cttccacggc gtttcggagc gagatctcct gcggtggtcg
     9361 ggcgaggcgg gagcctccgc ggcggccctg ttcgctctcc accagccctg cgtgggagag
     9421 tctggccagg atgcgggcca gcaggcgtcg tgggacgttc gcttcggcgg agatcctgtt
     9481 ggcgctcgcc tgctcgccga tttcggcgag gtataccagg gcccgcagcg cgtaacgtcc
     9541 ctcgctgctc aactccaacc tcattactca cggctctttc ggtagggcta aacggccacc
     9601 tcgaccacca gatgtattct agagataagc accggcccgc gctcccgtgt tcccggtgtg
     9661 gctgcttgtc cgggtctacc tgggctacct gtggtttatg gccggctttg gcaagctgtc
     9721 cgagccgggc tgatgaacgc cagcttcttc ttcgccggaa ccgccggggc caaccccctg
     9781 atgttcatcc tggccatttt gctgatgctc tcctggcggg tcgccgggta ccggggcctg
     9841 gaccgctggg tgttgccggc catcgggatc cctggctacc ccggaaccct tttcagcggc
     9901 cgcgcagagg gtcaacgctc ccgggtgaag taacagcggc cggtagcgaa cagcagtagc
     9961 aggtcccgtg gaagagagcg ccgctaggat gcggcgctct cttcatggct agctcctccg
    10021 acctctcccg ccaacgacgc caagaagggg ggagccggct ccgaccagga acccgaagtc
    10081 gtaccagtta ccgccccttt cggcgttgta gaacggaaac tgctcccaac cgccaaacac
    10141 gtgtacgact agaacgatcc aggccgtaag tccgttccag aacccccaca agaggtctga
    10201 ccaccacacc atgaacctcc tcgtccggga tcaatgcccc cgctctgcca cgcccgttct
    10261 tccgtttcag gatccgctgt ggggtcccat ccacattcta tcctgacacg gatcgagcac
    10321 ttccgtgctc gaagtgcgcc attcgctatc tgtcctttgc ggaacgcgcc cgcgaagaag
    10381 ttgttccccc tcctcggcgg acaacgtatc atggtgggct gtggctgttg gagactacga
    10441 ggcgatcttt gtcggcggcg gtctggcggc catgctgctc ttgcgcgaga tcggcccggg
    10501 attgtctggg cgggtcgcgg ttatcgaccc gtgcccgccg tcggaaaggc cgaccatcca
    10561 ctggagttac tggggccacg taccgaccct ctacgaccgg ttcgccgtcg ggagctggcg
    10621 gatggccagg gtggcgcaga tgccggctga gtccgttgca ccgtacaccc tgcgtctggt
    10681 gcgctcgacc gacgtgttcg cccagatagc tgaagaactg agcactgtgt ccctcgaatg
    10741 gctgcgtacc tcggcccatt cggtcgagcg ccgggaggat ggaacgtacg gggtcattac
    10801 ggacgctggt gcggtgcgtg ccggctgggt attcgacagc gtccccgaca tcgaacccgc
    10861 ttttccgtcc ccacgcaggc ccagggctgt gctgagcggc accggcgtcc gtgttacggt
    10921 cgatcggccg gtgttcgacg cggcggccgc aaccctgttc gatccgctcg acgaacggtc
    10981 cttcgcctac ctgctgcccc ttagccccga cgaagccctg ctcgagtcgg ccacgttcga
    11041 ctcggccatc cagaaaaagg acgagacgcc gctactgcgg tacctgcgct cccggtatcc
    11101 gggggccacc ttcgccgtca cacacgcgga gcatggccac ataccgctcg ggttcgcacc
    11161 atcgcaaact gccggacccc gccacgtgct gcttggcacc aagcgcggcc tcgtcaaacc
    11221 cagcgccggc tacggcgtgg tccgcatggc cctggagagc agacggctag cggggctctg
    11281 gaagcagggc cggccgctgc cgccgagcca acgatcctcc ctgtggtggc agctcctcga
    11341 cttggggttc ttgcagctgg ccgccaacga ctccaggcgg ccgctggctc tgctgcgcaa
    11401 tgtcatgcac gccgtgcccc tcgtccaatc gttgcgcttt atcgacgagg agcttccacc
    11461 gcgacagctc gcggctgtct tcggggccgc actcccggcc gtgctcggca gggtgtagct
    11521 gccacaggtt tcggacgcca tcctctagac catggtccgg gtacgcagct tctgggctag
    11581 cgccgatcaa agtcccgggt atgttagtcc ttcttcagag ccttccggaa cggggcgagg
    11641 gagaagctca cgtgggtcca catcccggtc tttctcttgg cgagcagcac cattatcacg
    11701 ttctgtaggc tcgtggtaat ggctgctgct gcggccaccc ccacgggtcc gtagcgcgtg
    11761 acggcgaata tggccaagac gaagaagagc gggctggtga gcaggctgac ccgcaacatc
    11821 gatccctgat ggcctgtgaa ctggagcacg agaccgcaag agccggacca gacggccacg
    11881 agcttggcgg cactgaggat caagagtacc aaccaggccg tgttgctgga gtagatgtcc
    11941 tggttgaaga gcaagcccag tatcggcccg cccagcagca tgaacccgac caagaccaac
    12001 agcgccggca cgccggccag agtgctaaag gcgcgtatcg tgcgctccag tctggctatt
    12061 ctgccctggg catacatctc cgcgacgatg ggcggcatca cgaggttgac caccaagagc
    12121 ggcatcacca cgaaggtgac cagcctggag gcgttggagt agacggcgac cgacgtctcc
    12181 gaatcaaagg cacccagtat ccagatgtca gccgtcagca agacgaacga ggtcatggcg
    12241 atccccagga acgggatggc gtcgtccagg acctctctcg cgctcaccgg ctcctcctcg
    12301 gtcgctttgg agccgaggga ggacaccctg ccgtagagca gccagcacga cagcaacacg
    12361 ctcaccgaac ccgctcctat ggagaccagc gtgacggtcc tcaagtccac gcttgcgccc
    12421 gtcaccacca gcagggccag ggcgccgagc agcaagagct tcatgatcag gccgccgctc
    12481 ttgccgcccg tcgccaggcc accgagaagg gtggcccagc ggaggtcgtg gaagccccgg
    12541 aacgtttcgg ccgttatctc ttgtacgatg gctatcgcca tccacacggc tatcagtccg
    12601 gtaagaccga gtaggagcgg agaatcgaat agccgcccct cgacgatgtc gcctatcacc
    12661 aggtagtagg cgaggctggt gagcagggtt cccgctaccc cgagcccaag cacggtgtag
    12721 attacccgcc gcgtgcgccc agagcgattc agccccatgt tctcggcgac caaccgtacc
    12781 accgtcttgg ggagtcccaa agccccgacg agcgcgccga aggagactat ggtgagtacc
    12841 agagtgtacg cgccgacttc ctttggggag atcacgaaag taagcaggct ggcggtgagg
    12901 atgctgatca aggatccgcc gaacctcccg gcgacggccc agatgctacc ggagagcaaa
    12961 cggcgcttga gtgtcgaacc actcaagaga ccgcccaata ccgacgattc cgagcgtggc
    13021 cgctcgtcag tcacttatca tccgagcttc tgtgggtaat accggccgac actaaagatg
    13081 tcaccttccg ttagggacct ccgaggcgct tggggcgggc ctttcttacc acgaggaaga
    13141 tcatgatgcc accccagcgc cagtctccga gtatcaattc taccgtgtct ggggaatgac
    13201 ctaccaggtc gcactagtcg tgtcgaactg actattttga cgatcaggta gcgcgtggag
    13261 aacgacgaaa ctgggagaag acggccgccg ccgaaggcag gatgaatgcg acgaggtccg
    13321 cgtccatgcc gtcgaaggtg tagcccagtc cgccctggaa gggcaagcgc cactgctgct
    13381 gcgccatcgg gagtctctgc gagaaagagt ctatgccgcg ttgggagctc agcatctctc
    13441 tcgcggccgt tctgcccaga tcgcccaccg atcgtgttct caagcgcgta gtgccagtcg
    13501 ctcctctgcg atggaaacgt ggctaggtcc gtccttcgga cagctcgtcc gtcctagccg
    13561 ccattatgaa gtcgttttgg tgcagaccac cgatcttatg ggtccaccag gtcaccgtca
    13621 ccctgcccca ttcggtcaac agtgcggggt ggtgcccctc cgcctcggcc tgctcgccca
    13681 ctctgttggt aaaagcaagt gcttgggcaa agttacggaa cctgaacacg cgctccaggc
    13741 gcttgatgcc ttctcgctgt actacctgcc acccggggac ctgcgggtgc atctgggcga
    13801 tttcggcgtc ggttagcgta ggctcgtcgc cccggcaggc ggtgcacttc atctccgaca
    13861 gctcgctcat gttttccacc tttctatgag ccagtgggtc ctagaactgt agtctccaac
    13921 ccgatagtaa gtcgaatctg cgcctagtgc ggtcgtattc cgggccagcc agcgaactcc
    13981 cgcacaaagg agaccacctc gcccgccgcc gcctcgaaca gcgcctcccc tttctccgcc
    14041 gtccccgtct ccggatggcc gaaagctccc gtctcgctga gctggtcgaa ggtgcgcgca
    14101 acgtccaccc tgctagggcg ggagaagtcc gggcagtaga acgccgactc gaacgggatg
    14161 ttcgccccgt gtgctgcccc tggcttcacc aactccggac gaaggctcaa gatcatgctc
    14221 gtctcctgct cgcaggcgtg tgagacgtgc tcctgttcta cctcctccag tgccgctatc
    14281 cgctccgccg cgaccgtcat ccagctcgcg aaggccagcc acagatccgt ctcctcgcgg
    14341 tgtttgagtt gcacgtcgta gatcgcctgc cgtcctggcg tgacgttgcc accgtgcgcg
    14401 ttgagcagga agacgcgccg gaaacccgcg tcgatgaggc tctccagagc gtccgtgagc
    14461 acccgaacgt aggtctcgtt gcggatgctg acggttccgg gaaaggcgcg gtggtgatcc
    14521 gacgccccca cccagagcgt cggcaggaac agcgcctcat cccccagctc cacctcggcc
    14581 cgccggacta tctccgtgca gatcatggtg tcggtcaaga ggggcaggtg atgcccgtgc
    14641 tgctcgaagg aacccagcgg cagcaccgcc acgcgctccg ttagcccctc gatctcgttc
    14701 cagctgtgct ctcctagccg cattcgttcc ctccattgcc tcgatctctg ctcctgaaag
    14761 cctgcagctc atcctaaggt tgcaaaccaa cacctgcttt gccccgcgct cggcgtacgc
    14821 gccgtggggc gtgtccgcac atacattgaa catcttcacc tttcggccat agcaccccgt
    14881 actctctgta tgcctccatg atacccgctc cctgctggag ggccttcttc atttcgctct
    14941 ctactttaga gagagcctcg agcagcacct ccttcgccgc gtgttcgtct cttcgctcgg
    15001 gaaagccgta tagggggcgg gttagagtcg gaagtcgccg cagagctcgc ctccgatctg
    15061 ctcgacctcg tccgtgaagc tccaggcctg cttctcgcat ctcagggtcc ccatcgttcc
    15121 ccgatgtttg tcctggcaac cgtcgaatct ctgtggaaga tgctctgaac gcacctgctc
    15181 cttatcgttg gttcatcgat gaccgccagt cagcatgtaa gattgcaacg aaatgggcgg
    15241 gactcgtggg atacgtacct accaaacgag ataaggcctg gcgagagcca gggtagcagg
    15301 taaaggaagg gcggacggcg aaattgataa cggcggtgag tcgcagagaa tttctgagac
    15361 gcgggggcgc tgggctcatc ggtgcgatgc ttctgggcgc cgcaggctgc aacagcagtc
    15421 acggtaaggt tgtcaggttc ttgaccggca ccagggagac ggctgccctt gaaagagcgg
    15481 caacccaatt acaagttgac ggtttcgaga agcaacaccc gaacatcgaa ttgcagcggg
    15541 agacgctaca gccggatgag gtccgcaggg agatagagag caggctgaga tccaaggagc
    15601 ctccagacgt cttcgcctac gacactggtc ctggcttcgg cggtgtgctg gccgacgcgg
    15661 gactactgcg ttccctggag aacgcctaca caaagtacgg ctgggacatc tacgactggg
    15721 ccaagcagca ggccacctac gagggtaccg tctacggcgt tcccgaccag gtcgaagaga
    15781 taatcgtcta ctataacaag gacctcatgc ccgaagtgcc acggacagtg gaagagctga
    15841 gagagatctc tggtgagctc atgggacggc gaaagatccc gctagccttc ggaaatcggg
    15901 aacggtatcc cgcgagtcac atgttcagca tcgctaccag caacgtgctg ggccgagagg
    15961 ggttggatag catactctac ggagacggta ggtgggacat cccccaggtg gtggaggcca
    16021 tcgagctgat gttcagggat ttcgtggaga gcggttatta cccgaagagt ccaaacgccc
    16081 tcacctacga cggcgccaac gccctgttct attccggtga ggccgccatg aaccccaccg
    16141 gcacctggct cgtgtcggag atcgtccatg ccgtccagga gtttgaagtt ggattcttcc
    16201 ctttcccgtc catagaaggc tccggcatct cgcctccggc cggagtgggg acgggttggt
    16261 tcgtggccaa tggcgccggt aacccccaag aggctttcac tttcatcgat tacttgctcc
    16321 aggacgacac cgcccgcctg accatcgaat tgctcaacac gattcccgcc caccccgttg
    16381 acaccgaagg gctcgacgtt cccgagctgt tcaagcaagt gctcgatgat ctttccgaat
    16441 atcctcgagc aaaggctttc ggctacaaca tagatgtcct gacgccgcag aacttcaacg
    16501 aggtgatgta caccggtttc cgggaggtca tgaacgggat gaggtctccc gcagagcagg
    16561 cagtagcgtt gccacgggct tggacgaagg cgaaagaggt gggaaagaca ccggcgcagg
    16621 agtaggccga gcatgatacc aaccggcccg acatgctatc agaagatcgt gcccaaccga
    16681 aaatagttcg aagatcatca atcggaggat gcaccttact ctgctcgtat gctgtatttg
    16741 ctactataca cggggagtga gtccccctca tgtccccatg agggggcggc gtccttccac
    16801 gagcgccggg agagaaggga gatggtgaga tgttcgcgcg ggtaagcaca tacgaaggtc
    16861 gcccggagca gttggacgag atgcaccacg agggcgtaga acacatccta ccggcgctcg
    16921 agatgcaaga cggttacagc gggggcctgg tcctcgccga tcgtcagagc ggtaaggtgc
    16981 ttgcgttgac cctgtgggag agcgagcaag cgatggacgc taccgaggag gcctctcaat
    17041 ggttgcgcat cttcagcgcc gaatccagcg gcgggacgat aagcagcgta gagaggtacg
    17101 aggtcttcta cgcggcgatt caggaagcgc caccttccga gcggtccggc gaaccgggaa
    17161 gcacggaaac caggcgtcct taggggacct cggggatcag gtagaagttt agccttggca
    17221 cgctaatctg atctctccag ggtcaccaag gtgatatcgt cctcctgttc ccaattctca
    17281 ccggtaaaat cggtgagctc ggagagaagg aagtcgatca gcgaagattc gcccgagtgg
    17341 tgagccccta ccaagccctg cagcctgggg aaaccgaaca tctcgccttt agggtcgtgc
    17401 gcctcgacca acccgtcgct gtaaaagaga acgctctcgc ccctctgtag ggtgacctcc
    17461 ttctcctcgt attccatccc cggcatcagg cccaagggca tccctctcgc cctgagctcc
    17521 tccacgtcac tgccattatg ccgcaggtag ggtgggtcgt ggcccgcgtt ggcatagacg
    17581 agacgcccgc tctccgtatc gaggatggca tacaagcagg taataaacat attgggtggg
    17641 atgtcaggga ccagcgcgtc gttagcccgc ttcaagacct cccccggtgg gaagtgtcgt
    17701 tgggcagaga cacgcagcat agtgcgggtc gtcgccatca ccagcgcggc cggtatgccc
    17761 ttgtcggtca cgtccccaac gacgagaccc aggcgtccat cttccagcac gaatgaatcg
    17821 tagaagtctc ctcctacctc gcgcgcgggt ttgtagtagg ctgctatgtc gtatgcagag
    17881 agcttgggca atgtcttggg caagagggtt tgttggatga ggcgcgcgat cctgagttcc
    17941 tgctcgatgc gctccctctc cttcgcctca cgcttctgct cggtgacatc gtactggata
    18001 cccaaccagt acaggggttg tccttcctca tcgcacacca gggtcgcctc atcgcgcacc
    18061 cacaccaccc ggccatcccc tgcgacgacg cggtactcta cgttaaagtg ttcaccggtc
    18121 tggtcagtgc gagcctcttc ggccaacacc cgctcccggt cttcggggtg caccgccctg
    18181 agccagtgtt cctcgtcgat catttgcgaa cccggggaaa atccgaggat ggtctcgtac
    18241 tgtgggctga cgtatgtcac ggcctttgga ttgctggact cgagggcctc ttgcacgtag
    18301 gtgatggccg gggtctgctc tacgagagtc cggtagcggg tctcggcttc ctgctgctga
    18361 cgcacgagcc gggcgactcg cacggccgga gccgtctggg tggagaggtc cttcagtagc
    18421 ttgcggtcgt cggccgagta ttcctgctgc gagaggcgcg gcccgaggct caagagaccg
    18481 atcagctcgc cctgacttac cagcggcacg accagctcga tctgcgccga cttcatcgcc
    18541 ccgagcgctg gggagtcgag gtctagcttc tcgacttcta ccacccccga gacgttcgaa
    18601 aggtacgcga ggatcgggtc gtccggcgct atctcgatct ccggctcctc tatcgccgtg
    18661 gtcgccaact ttgtagcctc cctggcacgc tcctgagcct tcaaccacag cgacacgcgg
    18721 gtcggctgca tcgtttccct tatcacgtcg atgagttcac tgctcagggc gtcgaggtcc
    18781 gtctctatcc gcagcttggc cgagaaggct tccagggtct ttctggcatc gtatttcctg
    18841 cggtagaagc gacggtctat gaacgactgg atgcgcagcc tcaaaggggt gaatagcgcg
    18901 gctatcgcca gggtcgagat caccaccgca agctgtgatt cctgaccggt gaaggcacga
    18961 aagaccattt ggaacaggac gatgctaccg aagtacaccg tcgcaagcac ggccgtgagc
    19021 gcaccgtaga cgagggttct atttaggagg aggtcaatgt tgtagagtcg gtaccgaagg
    19081 atcgctatgc ccaccgcaat cggcaatccc acgacactgg cgattaccag catggagctg
    19141 acccacccca gccacaccac ccccaccgat tcagagacta cataggcgaa gatggcgcta
    19201 gtagccagca ccgacacggc gtaggcgaac cactttacct gctggcgctg cacccccata
    19261 gagcggcgca ggcgtaccaa cagcgaggcc gctgctatga gcccgagggc gtacaggata
    19321 gtttccaccg ggtttacggt ctctgtcgcg acctctatgc cgagggggtg gttgacgaga
    19381 tcgaatcccg cagccgactc gggccagagc gctacagaga ccgtacctat tacgatcacc
    19441 gcaccaacaa gccacccaaa ggggcgccaa cgcgatgaag gaactctgcc atcagggaac
    19501 agcacggaca ggaacacaaa cagaccaata tgcgaaaccc acagccacga ggagatccaa
    19561 gccaaaacct ctccgcccgg cagcatggcc ggaaccgatc tgggctccgc cagcaacgtc
    19621 acgatggcgt actcggagac aaacaggcgc atccctgcaa tcacgcctat ggagcaaaag
    19681 atccagccta tggggttctg aggggggagt cggggagtga ttatggcccc taccgtcgaa
    19741 aagcttaccg cgataatggt gttttcgagc cagtagtcga agacgggggc gcccgcgtag
    19801 acgggaaccc ccacagggga gcggctcacg accaggagga agaggccgag caccgtcagc
    19861 gtcaaagaga cggcgccgag gcacctggcc aacaaggccg tcgcacgagg gctcagccta
    19921 agagctcctc gcccgaagct ctcctctcca gcgctcgttt tcacagtgcg tccatggtac
    19981 agccccaccg atcgttctag gcaacaatga tgacatttcg gacggccagt cgcatcccca
    20041 acttgcgcat tcttgcgctg acgtgcagag caacctccga gaagcgcctc cggtaggcac
    20101 tcgggaggat acagccaaga aagcgaagtg gagacgaggg gattcgaacc cctaacctct
    20161 gccgtgcgaa agtggcaaga tagattgcca gaggtttctg gagcttgcaa aatcctgcca
    20221 tgaagatggc cgcgatagtc aatccgaggg cactaaccag gagtaatgtc aggccgatac
    20281 ggccggctat ggtctataga tgtgagcgga gcgctaggaa caaacagaca tagccgagtg
    20341 ccagtacaag aaaggcgagc accattatcc agccgtactc tccgatcgcg tactcgctga
    20401 tgaagtgcta caaaggatct ggccctggtt tgatgaagtg cagcgcggca agcaggacta
    20461 cgaacgtggc ggctccggca aacgacaggc gtgccgctgt tgatgaaacc gctgccgccg
    20521 aatcgcattt ggctgtacct tcggccatcg ggttctcccc tattcgactc tggtcgaagt
    20581 cgcctcgtaa ccgccgcccc ccgggtagtg ccaggcgccg ctaatagttt tgtcgtcttc
    20641 gctgaaggtg cccctgtagt acgccgggga tcccctttct ccaccccaga tcgtgagggt
    20701 atctccctcg atttcgtaga cgtagtcgag agtattgccc atgttgtcgt agaagcgaga
    20761 cccgatgtcc tcgcttggtt cagctccgaa ttcccgctcg tgtccgatga tctcgatgcc
    20821 cttgatcttc tgcccgtgct gctggagatc aacgcgctgg atgaggaaga agccgccctc
    20881 catccactcg taggtagccc ttccttgcgc gcctcctgag atctcc
//
LOCUS       DATMVF010000268         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_29357, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000268 DATMVF010000000
VERSION     DATMVF010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29357"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VN178_13775"
     CDS             <1..199
                     /locus_tag="VN178_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82082.1"
                     /translation="SAAGGFFVMAAYSTLLNWLNRRYLPEYAKLKGWRSPVIVFVALF
                     YLLPTLYVTYLLVTQGMSAFA"
     gene            complement(492..1121)
                     /locus_tag="VN178_13780"
     CDS             complement(492..1121)
                     /locus_tag="VN178_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82083.1"
                     /translation="MEHWHGFETARNVAQVYETTIADTASDLSAHFVTVPARSLGGRS
                     YLFQGSEWSLETLGLLRIADVPFGGVARSLAACLVSDDVEEFLRKFDQGFEEETKHSS
                     VRLPKPFEFAEYLTFARVIPFEQPSLSADSLGNILTAQGHGEAGYVYHEATGTPLLAV
                     AIPAGVVICGSAPGMVRALESGLRQRLKEFIKSRSGEPQDADERIGQDG"
     gene            1380..2237
                     /locus_tag="VN178_13785"
     CDS             1380..2237
                     /locus_tag="VN178_13785"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWS82084.1"
                     /translation="MAELVELREAVTGLMSDGDSVALEGFTHLIPTAAAHEIIRQEIR
                     DLTLIRMTPDLIYDQMIGMGCARKLIFSWGGNPGVGSLHRFRDAVENGWPRPLELEEH
                     SHAGMANRYVAGASNLPFAVLRGYVGTDLPKVTSTIVPIKCPFTGEELVAVPALNPDV
                     AMIHAQQADRRGNVAIWGISGIQKEAALSAKRVIVTVEEVVEKFEPRPFQIVLPAVAV
                     DAIAVEPGGSRPSYADGYYDRDNAFYEAWDGISRDRKRFGEWMERHVLGTRDFAEYTK
                     FIESEETLR"
     gene            2234..3019
                     /locus_tag="VN178_13790"
     CDS             2234..3019
                     /locus_tag="VN178_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HWS82085.1"
                     /translation="MTDNGALEYTTDEMMTVAASRELEDGAVCFVGIGLPSEATNLAR
                     ATHAPNCLLIYESGTIGAKPEVLPLSIGDGVLAEHADSVVSVSEIFNYWLQAGRVDVG
                     FLGAAQMDRYGNINTTVIGTYESPKVRLPGAGGAPEIAASAREVVIVLRQKPRSFVED
                     LAFVTSVGHRHGGDSRGELGYGGAGPSMVITDLGILRPDPVTKELTMTALHPGTTVEE
                     AKEATGWELEVAAKVETTDPPTERELEILRDLKARTEASRKKA"
     gene            3116..>4016
                     /locus_tag="VN178_13795"
     CDS             3116..>4016
                     /locus_tag="VN178_13795"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWS82086.1"
                     /translation="MSYGGTTEAWIVGAVRTPIGRHGGSLSAVRPDDLGAVVLEALAD
                     RTGVPAELVEDVYFGCANQAGEDNRNVARMSLLLAGFPEEVAGVTVNRLCGSGLDAVA
                     QAARAVMLGEADVYVGGGVESMSRAPWAVPKPERAFPTGKATMYDTTLGWRFVNPKME
                     ELGHTDSLGMTAENLAQERFLLTPDEDEPDEVAIAYDVSRVAQDRFALRSHEKAVAAM
                     DEKRFREEIVPVTVKTRKGETVVEADEGPRRDTSVEKLGKLKPAFKGDGSVTPGNSSS
                     LNDGAAAVLVVSREYAEAHGLEPL"
BASE COUNT          752 a         1259 c         1342 g          663 t
ORIGIN      
        1 cagcgcggcg ggaggctttt tcgtgatggc cgcctactcc acgctgctca actggctcaa
       61 ccgccggtac ctgccggagt acgccaagct caagggttgg cgatcaccgg tgatagtctt
      121 cgtcgccttg ttctacctcc ttccgacgct gtacgtgacc tacctgctgg tgacccaggg
      181 aatgtcggca ttcgcctgac cagggaggga cgcggcaacc cggggagaag gcgcagccgg
      241 cgggcctctc tcgaatcccg ctcagcccta gcttgggtct agccgaacct gatcttcgcc
      301 ggccgaactc ttactctgaa cgccgtccct gaccacccga acaaacgagt gcagaacagc
      361 gcctgggtcg tcgcgacgcc agaccttgcc cagttccacc gtgggcgaga ggccgtatca
      421 tgaccagggc accgatcatc tcgcgatgcc ggacgaaacg ccaccaggtc aggcgagcgt
      481 tgcccgagag gctatccgtc ttgcccgatg cgctcgtccg cgtcttgggg ttcgccggag
      541 cggctcttga tgaactcttt gagccgttgc ctgaggcccg actcgagggc tcgcaccatc
      601 cccggggccg aaccgcagat caccacgccc gccgggatcg cgacggccag gagcggggtt
      661 cccgtcgcct cgtggtacac gtagcccgct tcgccgtggc cctgcgccgt caggatgttg
      721 cccagggaat cggccgacag cgaaggttgc tcgaacggga tgaccctcgc gaacgtgagg
      781 tactcggcga actcgaaggg cttgggcagt cgcacgctgg agtgtttggt ctcttcttca
      841 aagccctggt cgaacttccg caggaactcc tcgacgtcgt cggagaccag gcacgcggcc
      901 aggctgcggg ccacgccgcc gaacggcacg tccgcgatgc gcaacagccc caacgtttcg
      961 agggaccatt ccgacccctg aaacaggtaa gacctgcctc ccagcgaacg cgcggggacc
     1021 gtgacgaaat gggccgaaag gtccgacgct gtgtcagcga tcgtggtctc gtatacctgc
     1081 gcgacgttgc gggccgtctc gaaaccgtgc cagtgttcca tacccgcaga ttatctctta
     1141 tcttcgcgcc atgcgcctgc gccatatcat cgaggtatgt ccgaggcatt catttggtat
     1201 tggacatata ccgcgctagc gaccagaatg acgaaagtga taggaaggag caacacagaa
     1261 gggcgccaag cgtcgacggg cgatcggata gtggggagag tcgatgtcca ggcacttcga
     1321 tcccccgctt gcgggttgac ggaaggagga tgcggcgagg atgcgggtaa gggatcggca
     1381 tggctgaatt ggtagagttg cgggaggcgg ttaccgggtt gatgagcgac ggcgactctg
     1441 tcgctctaga gggcttcacg catttgatcc caaccgcggc cgctcacgag atcatcaggc
     1501 aggagataag ggatcttacc ctgatacgga tgacgcctga ccttatctac gaccagatga
     1561 tcgggatggg ttgcgcccgg aagctgatct tctcctgggg cggcaatccc ggcgtgggct
     1621 ccctccaccg cttccgcgac gccgtggaga acggctggcc gcgtccgttg gaactggagg
     1681 agcactccca cgccggcatg gccaaccgct acgtcgccgg ggcttcgaac ctccctttcg
     1741 ccgtcttgag gggctacgtc ggcaccgacc ttcccaaggt tacgtcgacc atcgtcccca
     1801 tcaagtgccc cttcacgggc gaggagcttg tggccgtacc ggcgctgaac cccgacgtcg
     1861 cgatgatcca cgcccagcag gccgacagac gcggcaacgt ggccatctgg ggcataagtg
     1921 gcatccagaa ggaggcggcg ctttcggcga agcgcgtgat cgtcaccgtc gaggaggtag
     1981 tcgagaagtt cgagcccagg ccgttccaga tcgtgcttcc ggccgtggcc gtcgacgcaa
     2041 tagcggtcga gccaggcggt tcgcgcccgt cctacgccga cggttactac gaccgcgaca
     2101 acgccttcta cgaggcttgg gacgggataa gccgcgatcg gaaacggttt ggggagtgga
     2161 tggaacgcca cgtgctcggc acgcgagact tcgccgaata cacgaagttc atcgagagcg
     2221 aggagacgct caggtgacgg acaatggggc gctggagtac accacagacg agatgatgac
     2281 cgtggccgcc tcgcgcgagc tcgaggacgg cgcggtgtgc tttgtgggca taggcttgcc
     2341 gagcgaggcg acgaacctgg cgcgggcgac ccacgccccc aattgtctcc tgatctacga
     2401 atctgggacc ataggcgcca agcccgaggt cctcccgctc tccatagggg acggggtgct
     2461 tgccgagcac gccgactcgg tcgtctcggt gtccgagatc ttcaactact ggctgcaggc
     2521 cgggcgcgtt gacgtcggct tcctcggggc cgcccagatg gaccgctacg ggaacatcaa
     2581 caccacggtc atagggactt acgagagccc caaggtgcgc ctccctggcg cgggcggagc
     2641 tcccgagatc gcggcttcgg cgagggaggt ggtcatcgtc ctgaggcaga agccccgctc
     2701 tttcgtcgag gatctcgcct tcgtcacctc ggtcgggcac cgccacggcg gcgactcccg
     2761 cggggagctc ggatacggcg gcgcaggtcc gagcatggtt atcaccgacc tgggcatctt
     2821 gaggcccgat cccgtgacca aggagctcac gatgaccgcc ctgcaccccg gcaccaccgt
     2881 ggaggaggcg aaggaggcga cgggttggga gctcgaggtg gccgcaaagg tggagacgac
     2941 cgatcctccg acggagaggg agctcgagat cctgcgcgac ctcaaggcga ggacggaggc
     3001 gtcacggaag aaggcctgag gaggaccatg gaggatcccc caacgactcg attttggtca
     3061 gatttgtatc cgggagcggt gccagaaaac ggaaacccaa gatgagggga gatatatgag
     3121 ctacggtggc accactgaag cttggatcgt gggcgcggtc cgtacgccta tcggtcgtca
     3181 cggtggctct ttaagcgccg ttcgtcccga cgacctgggc gcggtcgtcc tcgaagccct
     3241 ggctgatcgt accggcgtgc ctgcagaatt ggtcgaggac gtctacttcg gatgcgccaa
     3301 ccaggccggc gaggataacc gcaacgtcgc caggatgagc ctgctcctgg ccggctttcc
     3361 cgaggaggtc gcgggggtca ctgtcaacag gctgtgcggc tccgggctgg acgccgtcgc
     3421 ccaggccgcg cgcgccgtga tgctgggcga ggccgacgtc tacgtgggag gcggtgtgga
     3481 gtcgatgagc cgtgccccct gggcggtccc gaagccggaa cgcgccttcc cgaccggcaa
     3541 ggccacgatg tacgacacca ccctgggatg gcgcttcgtc aacccgaaga tggaggagct
     3601 gggccacacc gactccctcg ggatgaccgc cgagaacctc gcccaagaga ggttcctgct
     3661 cacgcccgac gaggacgagc ccgacgaggt ggctatcgcc tatgacgtct ccagggtggc
     3721 acaggaccgc ttcgccctaa ggagccatga gaaggcggtc gccgcgatgg acgaaaagcg
     3781 ctttcgcgaa gagatcgtgc ccgtgacggt gaagacacga aaaggagaaa cggtcgtcga
     3841 ggccgacgag ggccccaggc gcgacacctc agtggagaag ctgggcaagc tcaagccagc
     3901 cttcaagggc gacggtagcg tcacgcccgg caactccagc tccctgaacg acggggccgc
     3961 ggcggtgctg gtagtttcca gggagtacgc cgaggcgcat ggcctcgaac ccctgg
//
LOCUS       DATMVF010000269        40081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_412680, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000269 DATMVF010000000
VERSION     DATMVF010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 40081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40081
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412680"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1511)
                     /locus_tag="VN178_13800"
     CDS             complement(318..1511)
                     /locus_tag="VN178_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWS82087.1"
                     /translation="MPEDLAVFIDWENIYISTVTEYKSKPNVSAILEKAREYGRIVAA
                     SAYADWTDGDFRQAPPTLYSNGISPRYISARYFPGGKSQKRRTNSIDVMLAVECSAFL
                     HDHPQVDTYVLVTGDGDFIPLVSLLRSKGKNVVVIGVSEATSYHLIESADHFVSYASL
                     LEEDRAARAQDREPKKEAADPYKELVRAVQALKKGGKTRVLGQVKQQMIVQLGSFDER
                     NAGFKKFKDFVVEAERRGLVNTVDQDLELQVYLPDEQVPDLQETDLAAVEPTSQSQPA
                     NRNRGNGRPAREERQQVEPEPGFDLLEDLTPYELKTVCTSVASLEQPATLVEIFSSIR
                     DLDERGELELSREEIGDVITAMLDADYLGKVRQKPYKQAKADMTFYALNAEKQEVKSA
                     LQHFS"
     gene            complement(1568..1981)
                     /locus_tag="VN178_13805"
     CDS             complement(1568..1981)
                     /locus_tag="VN178_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82088.1"
                     /translation="MHSECEGVVECTALVLAESCTEYEGRLTLFGVLDEIRPNGAGGT
                     SFVVYFKFEGCEHVSDRERRARVLVTDEDGEVLMESAEYSVYLDGTEDPVTKVAAFDL
                     PEGFEVGERLLWIEAVLDEETLAQTAVRIHDRYSV"
     gene            complement(1974..4538)
                     /gene="gyrA"
                     /locus_tag="VN178_13810"
     CDS             complement(1974..4538)
                     /gene="gyrA"
                     /locus_tag="VN178_13810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWS82089.1"
                     /translation="MLDTFSGNVQPHSIEDELKDSFLSYAMSVIVSRALPDARDGLKP
                     VHRRVLYAMQDLGLQPNRPYRKSATVVGDVMGKYHPHGDQAIYDALARMAQDFALRYP
                     LVDGQGNFGSVDGDPPGAMRYTEARLTRMATEMLRDINADTVDFVPNFDESLKQPEVL
                     PARFPNLLVNGSDGIAVGMATKIPPHNLGEVVDATIALIDDPELDDEGIAEHIKGPDF
                     PTGGVIVGLRGIRDAIATGRGSVRVQAKAHTEQIKGNRTQIVVSEIPYQVNKSYLLQK
                     IAELVKDRKLQDISDLRDESDRNGMRIVIELKREAVPKVVLNNLYKHTQMQQSFGVNL
                     VALVDGVPRTLSYRQALKHYIVHQFEVVTRRTRYELGRAKARAHILEGLLIALGDLDE
                     VVATIRRSRSVETARKNLMKGFGLSERQAQAILDLRLQRLTAMERQKVQQEHRDLREK
                     IEYLEGILADESKVYGIVKEELGEVKAAYADDRRTAITAEEGVDFEIEDLIAEEEMVI
                     SISNGGYLKSVPVNSFRKQGRGGVGVAGMELKEGDYIDHLFITTTHHYMLFFTNKGKV
                     YRLKVHQLPRMGRAAKGRHIANMLPLAQGEHIASVLATKEFEEAEYLLFATKRGIVKK
                     TRFQEYNTPLKNDGIIAINLAGDDELISVRYASEGDEVVIVSRNGKGIRFLQTDARPM
                     GRATAGVKGITLGKDDVVLSMDVISDDAADLFILTERGFGKRTALSQYRAQGRGGQGV
                     IAMKTDGERGKLAGVRVVRPGLHELMIVSNFGTTIRMDADSVSRQGRAAQGVRVMNLR
                     SGDSVSAIAKVISSRSADADGATDELTLDELSGEDQTGTSTAGLPEADGNGTVPGA"
     gene            complement(4531..6468)
                     /gene="gyrB"
                     /locus_tag="VN178_13815"
     CDS             complement(4531..6468)
                     /gene="gyrB"
                     /locus_tag="VN178_13815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HWS82090.1"
                     /translation="MAAKSMTKNAKSAYDASSIQVLEGLEAVKRRPGMYIGSTGPRGL
                     HHLVREIIDNSIDEALAGHCTEVLVTIHPDNSVTVADDGRGMPVGKMAKYGKPAAEII
                     MTTLHAGGKFGGEGYKVSGGLHGVGASVVNALSEWLELEIKVDGHVWSQRYERGFPKG
                     DLKKGRKLKKGEGTGTTTSFKPDPEVFTETQDFSFDTLSRWFREWAFLNKGLKIRLID
                     EREEDRDVTYQYEGGIRDFVEHINEAKDPVHKTIFYLEREEEVGDVEVAMQWNSGFQD
                     SVFTFANNINTHEGGAHLSGFRSALSRTINAYARQKGLLKEKEESLTGDDIREGLAAV
                     ISVKLREPQFEGQTKTKLGNTEVKGLVESVTNRYLAEFLEERPGEAKAIVNKALQAAR
                     ARLAARTVREKIRKGYLESSTLPGKLADCSSKDPARSELYIVEGNSAGGSAKGGRDRN
                     FQAILPLRGKILNVEKANINKILSNAEIQALISAIGAGVGESFDLEQARYNKIVIMAD
                     ADVDGSHIRTLVLTFLYRNMRDLIEAGYVYIALPPLYKITHNRREHYAKNDTELRETL
                     DRIRANGNASISRFKGLSEMNPQQLWETTMDPQNRTMLQVTVESAAVADELFTALMGD
                     KVEPRKQFIEENAREVKNLDA"
     gene            complement(6625..7749)
                     /locus_tag="VN178_13820"
     CDS             complement(6625..7749)
                     /locus_tag="VN178_13820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HWS82091.1"
                     /translation="MSAYLRALRLVNFRNYVDATALLAPGLNVVVGDNAQGKTNLLEA
                     VAFNVTGSSPRTPKENEVVRWGAEFARTEARVALDGSPGERGVAIGYAPGQKKRITVD
                     GVPAPSLTNFAAGGAGARVVTFFPDDIRVVKGAPADRRAFLDALLSSLRPAYARAALE
                     YAKAVQQRNQLLRRIRDGFSSERTLATWDKKVVELGKTVLAGREAALPVLEEHFTDAL
                     RALYGPEKAAIRYSHSSTLEEYSEALREAHSADVERGTTSVGPHRDDFEVSLGGVNLT
                     TFGSQGQQRIATLALKFASRDYVRGEVGEDPILLFDDVMSELDERRREYLAGYFLEST
                     QALISTTNLEYFDEEILRRARIIRISGGSILETATDGASR"
     gene            complement(7746..7940)
                     /locus_tag="VN178_13825"
     CDS             complement(7746..7940)
                     /locus_tag="VN178_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HWS82092.1"
                     /translation="MRLPPGTTLGRALKAAGLVGTGGEAKILIQAGEVSVNGEVETRR
                     GRTLQEGDVIEVGDERLEIG"
     gene            complement(7944..9077)
                     /gene="dnaN"
                     /locus_tag="VN178_13830"
     CDS             complement(7944..9077)
                     /gene="dnaN"
                     /locus_tag="VN178_13830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWS82093.1"
                     /translation="MRAVCSTETFGKKLALVSRGVSARSTIQLLGGILLEGRGEVLGL
                     SATDMEISIQTSSVAEVEGEGRVVIPARIFNDIVRSLPGGSFSLEHDDSEGTVRLVAG
                     ENEYRIRAYAADDYPRLPAFPEEGTFRMSGSSLVETVEKVSRSYSRDETRPVLTGILI
                     SFEDSRVRMVTTDSYRLSIKETELATTSFEGSREAIIPARAMQEVSRIFSGSDEEDVE
                     VALSENQALFRIGDVVLGTRLIDGNFPEYRRLLPGGFEREISVSREELVGTLRRVNLF
                     AARQTPPVPVSLSFSEGSVEVIVRNGEVGDAHERLSASSEDEFLISFNPGYLLDGVSA
                     IDSENVVFKLNEPLKPGLIVPEENGDEPDFLYLIMPMRDPSRS"
     gene            complement(9591..10934)
                     /gene="dnaA"
                     /locus_tag="VN178_13835"
     CDS             complement(9591..10934)
                     /gene="dnaA"
                     /locus_tag="VN178_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWS82094.1"
                     /translation="MAGKAEQVWTEVLDRASEHIDVSSLRVWFEGIRPVALYDDQLEI
                     SVPNTFAKEYIESRFRPLLEEVLDDVVGQETSLVVSVGGPGVHTGNGTAAEGAESVLN
                     VRTPRSFRAKYTFDTFVIGAGNRFAHAATLAVAETPGLVYNPLFIYGGVGLGKTHLLR
                     AVGHYVEDQDPSMRIRYVTCEQFTNDFINSMRDNAPLEFQKRYRENDVLLVDDIQFLE
                     NKVETQEAFFHTFNALYEENKQIVIASDRHPRYIQTLENRLVSRFEWGLVTDIQPPDL
                     ETRIAILRKKALMDHLEVDDDVLTFIASKVSTNIRELEGALVRILAYASLYGRQVSAD
                     LAEEVLRDILPDQAYREIPIELIQHEVCRYFGVAKADLVGQSRSKGFAYPRQVAMYLS
                     RELTDESLPKIGKAFGGRDHSTVMHATAKIANLINSDRDVFSQIHEITHQIKSKR"
     gene            complement(10937..11518)
                     /locus_tag="VN178_13840"
     CDS             complement(10937..11518)
                     /locus_tag="VN178_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWS82095.1"
                     /translation="MVENLIHAGYQGVVISVLDDGNVVRVSDKGPGVENKSRAMEFGF
                     SGAAPEALGEIRGVGAGLGIARAAAEKSGGTLTIEDNIGGGTVATISVAGDEAASKEV
                     APPPPQRRYPDGVPRMNISERQQKALITVLECGEVGPSTVADRLEISVSTAYRDLSVL
                     EEHGLVMADESGKRLISPLGRDLVGAIINSWVK"
     gene            12093..12227
                     /gene="rpmH"
                     /locus_tag="VN178_13845"
     CDS             12093..12227
                     /gene="rpmH"
                     /locus_tag="VN178_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWS82096.1"
                     /translation="MKRTYQPNRRKRAKTHGFRKRMSSVGGRRVVANRRRRGRKRLAV
                     "
     gene            12231..12587
                     /gene="rnpA"
                     /locus_tag="VN178_13850"
     CDS             12231..12587
                     /gene="rnpA"
                     /locus_tag="VN178_13850"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWS82097.1"
                     /translation="MARSKRMRLTDSPEFERVYRQGTAYRGRLFSVHAFPNERGTPRL
                     GLSVSRKVGNAVTRNAVRRRLREVFHSCISGASENLDLVVSARPAAAEATFEELREEF
                     GKSLGRVGGPVGRRPA"
     gene            12600..12815
                     /gene="yidD"
                     /locus_tag="VN178_13855"
     CDS             12600..12815
                     /gene="yidD"
                     /locus_tag="VN178_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWS82098.1"
                     /translation="MTARRASTGLIRLYRRYLSPMLPPSCRFTPSCSLYTLQAIEKYG
                     VPKGIFMGVLRVLRCHPFSKGGFDPVR"
     gene            12826..13683
                     /locus_tag="VN178_13860"
     CDS             12826..13683
                     /locus_tag="VN178_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWS82099.1"
                     /translation="MINAIADFFQNLFSPIVSVLGAVLLYFHQTLGAPWWLAIVLLTV
                     IVRSLLFPLTIKQVKSMRAMQDLKPHMDRVRAQFKDNPQRQREEMAKVYQEQGVNPLG
                     GCLPILVQMPIFIGIFYVIRQFGGYSLGGRTVEAQYPSFHEGGILWFQNLSIADPTYL
                     LPIISAVTMLAATEITAKNIDPQQRWLMRLLPIGFTIFLLNFPAGLFVYWITSNLVTL
                     GQNFVIYNYGPGKKSTDVAKATDGVKSKSKNGQTRNSDDGPPPERSQEEIAKQAAKVA
                     KRKRRKKKK"
     gene            13680..14462
                     /gene="jag"
                     /locus_tag="VN178_13865"
     CDS             13680..14462
                     /gene="jag"
                     /locus_tag="VN178_13865"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HWS82100.1"
                     /translation="MTDSVEFYASTVDEAVEKAALELNLPLAEVSYRVLDEGNSGFLG
                     IGARDARIAVEVPAHHTPDEDNEPPETTAAQDVAVDEPEPAPAESTGSSTLSEVYGEQ
                     TESEQIVAPEELLEEIRTLVSSVLEAMGFEARIEVYDAGGFIAADVAPDNTALFIGQK
                     GETIDALQYLVNVSAFRERPYFKRIVLDAEGYRQRRVEAIQGMAHRTARRAVRERRTV
                     EMPPMNSSERRVVHLYLSENPGVTTQSEGSGDSRRVKVSPTT"
     gene            14601..15311
                     /gene="rsmG"
                     /locus_tag="VN178_13870"
     CDS             14601..15311
                     /gene="rsmG"
                     /locus_tag="VN178_13870"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWS82101.1"
                     /translation="MEVQAAAWGLRLGGERREHLKEYAGLLACYDRANVIGTRDVDRI
                     LVDHVLDSLSCFLQEALFRAGRLADVGSGGGLPGIPIKIVRPDLATTLVESTGKKAAF
                     LRRAVEDLSLRDVEVANTRVEDLGGTSAHRGAYDVVTCRAVASLSVVAEYCVPLLRTG
                     GCAITMKGRLDPEELAEGHGALKALGARVAETTRVPLLPEVGEKERNLVILEKTRETP
                     PRYPRRAGIAAKRPLGIR"
     gene            15336..16100
                     /locus_tag="VN178_13875"
     CDS             15336..16100
                     /locus_tag="VN178_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWS82102.1"
                     /translation="MPAVRTVVAIVNQKGGVGKSTTAINLAAYLAGKGERVLVVDMDP
                     QGNATSGLGVLPGPGGCMYDVLLEGKPLRDVAVKTEIEGLDVAPATINLVGAEVELVS
                     SLAREFKLKRALERLASYDRVLLDCPPSLDLLTLNALTAADEVLIPIQCEYYALEGLT
                     QLMQSIRMVREELNPDLEVGGVLLTMFDPRTNLAHQVADEVRSFFGERVFHTIVPRNV
                     RLSEAPSFGMPVALYAPKSTGAEAYSAVADEVVNRG"
     gene            16093..16959
                     /locus_tag="VN178_13880"
     CDS             16093..16959
                     /locus_tag="VN178_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWS82103.1"
                     /translation="MGKRGLGRGLSALIATGESVGGLKFEELPVSAIRPNSRQPRTSF
                     PEAGIRELAASMREVGILQPLVVRPTPGGFELIAGERRLRAAKEAGMERVPVLIRQAG
                     EDESMELALVENLQRENLSPLETAAAYQALMDGFGLTRERLARRLGKSRAAVTNTLRL
                     VHLPERIQVLLGEGKLSEGHARALLGLETEEQMVRLAQRVVAEKLSVRKTEELVREML
                     GNPGETPSATTRRDSANPDEFHRASRLMREALELPVRVKGLKSGGKVEIRFRQTEELE
                     ALVSLLTAQDDN"
     gene            17225..18439
                     /locus_tag="VN178_13885"
     CDS             17225..18439
                     /locus_tag="VN178_13885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HWS82104.1"
                     /translation="MSGGQLRVWSVLGVLSFALVLAGAEGMARAQSEDDDASAAGSAR
                     VPLLEDKISDQDAALRGMIQEISTVGAELDRAQSRVDDAGSRVDDLRRQTRQLKRQIA
                     DQDAAFREAQAGYRAQARAAYKGGSLEGLAALLDGWFFDSWEGVAGTDDPVVAGVLLD
                     SRQNLAAYRESRQTLRNTQRQIAQKRRDYNAAIEEQQATTKDLRLRKQALEGAIARLS
                     TNNARTETRLQRLEAAERARIQKSVAATGRGWVGSEHELEVAREDIVATNVEPASEDA
                     YMELYEAAAANYGFGPDWYILAAVGKVESDHGQAMGPSSAGAMGPMQFMPTTWEASGV
                     DGNGDGVANVMDPEDAIPAAASYLQDGGAPQDWYRALYSYNHADWYVKKVLAVAEAYR
                     RLTNDESVGPYI"
     gene            18642..19436
                     /gene="modA"
                     /locus_tag="VN178_13890"
     CDS             18642..19436
                     /gene="modA"
                     /locus_tag="VN178_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWS82105.1"
                     /translation="MVSGVSLAGVAGCGGVSGREDGGGGRPQGGTLTVLAASSLIDAF
                     GELANRFEEQNPGVEVRQSFESSSTLLAQIQQGAPADVFASAAEEEMNTAVKDGLVEG
                     EPEVFVKNREIVMVPKDNPANIRRFRDVAKPDVKLVLAGKDVPAAEYALEILGKAESE
                     YGADFAEDVLSNVVSRESDVRASVNRVVVGDADATFGYASDYTPDIRDRVKVVPIPPD
                     LNIVATYPVAALEDAKSPELAKKWVELVTSEEGQRVLQKWNFEPAA"
     gene            19433..20263
                     /locus_tag="VN178_13895"
     CDS             19433..20263
                     /locus_tag="VN178_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS82106.1"
                     /translation="MKRRGKAGSTTRLPSGSDTFASGLAVASLALLAGFISLPLLSLV
                     VWTVDQGAWRAMASPVAVDALLLSVRTTAVTMAILLLVGTPAAYVLARAEFRGSRLIN
                     TLIDIPVVLPPSAAGIALLLAFGRLGLVGQYLNVFGITLSFTTVAVVMAEVFVAAPFY
                     VRQAATGFGAVDRTVEEAAMVDGAGRWRRFFRVTVPLAFPALVAGALTAWARALGEFG
                     ATIIFAGSFRGVTQTIPLAIFAEFQRDLDAAVALSVLVLGFAFAVILAVRYLTGRPTG
                     "
     gene            complement(20260..20481)
                     /locus_tag="VN178_13900"
     CDS             complement(20260..20481)
                     /locus_tag="VN178_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWS82107.1"
                     /translation="MQLSTRNRLPGTITEVVKGEAAAKVTLQVGDNHLVALITRESAD
                     ELGLEAGKQATALVKATDVMILTEGGEVV"
     gene            complement(20462..20785)
                     /locus_tag="VN178_13905"
     CDS             complement(20462..20785)
                     /locus_tag="VN178_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS82108.1"
                     /translation="MDVEPLLQALAEGTALEPFRALIEPLREHDRTRRSDLVLTLKTY
                     FSAGGNASEAADRLFLHRNSMLYRLERIQKLTGLDLKNDRVALALQLGLLAIERGERD
                     ATEHP"
     gene            20861..21196
                     /locus_tag="VN178_13910"
     CDS             20861..21196
                     /locus_tag="VN178_13910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS82109.1"
                     /translation="MNASRTDMFRKLLQRDPTNPMVLYSLGNELFKEGSYGEARDHLQ
                     RAVANKPDYSVAYRMLGRAHYELHENEEAKNIFLKGREVAQNNGDLQTVKEIDVFLRR
                     LHKREVGEG"
     gene            21223..21669
                     /locus_tag="VN178_13915"
     CDS             21223..21669
                     /locus_tag="VN178_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82110.1"
                     /translation="MSEARVGIFVDYESPLASRVAGDVWSGLSRAALQAGFTKTTAHW
                     ESLREAQVPKEGPPLRVFAIGQDRPDAIDAVVAVGDPGAPHLYGVIVAVPSKPSPLAG
                     SLLYQGVERLTGTGVKAGMIEPALLHAVPAECERYARRLLERLGDP"
     gene            21677..22372
                     /locus_tag="VN178_13920"
     CDS             21677..22372
                     /locus_tag="VN178_13920"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HWS82111.1"
                     /translation="MVSQLLTAILVTGAFAALAYALGMINRSGALGGLLVGTMIYASL
                     GPRGFAILALFVIGGSLLTRLGYASKARTGTAQEHGGRRSARNALANCAVATFCAILA
                     AVTGSEPFVAAFVASLGAAFADTAESEIGQLLSRTPRLITTLQRVPPGTDGAVSFPGT
                     LAGVGAAGLTAALGLVLGMLETPAGLLLVSTAAFLGTIVDSLIGALSPRAGNELTNVL
                     CTLVAALLAIIFA"
     gene            22710..23438
                     /locus_tag="VN178_13925"
     CDS             22710..23438
                     /locus_tag="VN178_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWS82112.1"
                     /translation="MDANKYEVLSFDCYGTLVDWESGIISGLRPVLRSHDVEATDDEI
                     LALHAQTEHKLQAASERDGYVKYRDVLGEEVREAGREWGFEPSPSEVEALPDSVRYWL
                     PFPDTVNALRALKGRYRLAIISNIDDGLFALTARYLEVEFDYIVTAEQAGTYKPSLNN
                     FELALDRIGVAPEKLLHVAESLFHDHVPAKEMGLDTAWIHRRAAKGGFGATPPAEAEP
                     DLEVPDLESLVHALGLRGSEGDRY"
     gene            23778..24794
                     /locus_tag="VN178_13930"
     CDS             23778..24794
                     /locus_tag="VN178_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HWS82113.1"
                     /translation="MSIENPALSVSATDYHTAGEPFRIVSGGVGPILGKTMLDKREWA
                     IENLDRVRRLLVYEPRGHADMYGGFVTEPDDQAADLGVLFFHNAGFSTACGHGTIALA
                     AWAVESGIVVPSEPETEIVIDVPSGRLPVVAKIEGGRATSVRFRNVPSFVHSRGISVR
                     TRSAGEVPLDVSFGGAFYASVEVSALGLAVEPADLPRLVEVGREIKDELNATMEIVHP
                     LEPRLRDVYGTIFFEDVEGPGFAQRNVTVFADGEVDRSPCGSGTSARLTLLHAGGELA
                     LGATLGHFGVVGTRFEGRVVEETKVGDFAAVVTEVEGSAHLTGHHQFVLGSDDPLGPG
                     FLLR"
     gene            24791..25957
                     /locus_tag="VN178_13935"
     CDS             24791..25957
                     /locus_tag="VN178_13935"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWS82114.1"
                     /translation="MSGPSVVRGINSIKRPRSPVAVLITVFTLAACGNGGQDPAAPAT
                     EPRREDPSVEEFLDDTLPDGASGTVVAARDGEIAHCRGFGLADREAKVAAGCDTAYDI
                     MSMTKQFTAAAILKLEMMGKLSVSDPMSEFVGPVPDDKRAITVHHLLTHTSGLTDQLG
                     GDYEALSREEMLDGALESELRSAPGTEYSYSNLGYSILAAIVEKASGTSYERFLASHL
                     FEPAGMRQTGYVLPAWKPDQVAVEYDENGEPKGKPFDHPWAEDGPYWNLRGNGGLLST
                     ARDMFRWHAALEGDQVLSESAKDKMFESHVPEEEGGDSYYGYGWVVSPTDEGRIVWHD
                     GGNGWSFGVMARFADQDTVVFWVSNHAYNDGEWNLENLAQRLTLGIAERVLDDG"
     gene            complement(25989..26207)
                     /locus_tag="VN178_13940"
     CDS             complement(25989..26207)
                     /locus_tag="VN178_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82115.1"
                     /translation="MWFVIVSGAVITVWFTYLFAMRSFKLHAVATGALTVVVVLLLFT
                     IGVLDHAYDGDVRVGPGAFELALQEMER"
     gene            complement(26498..26674)
                     /locus_tag="VN178_13945"
     CDS             complement(26498..26674)
                     /locus_tag="VN178_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82116.1"
                     /translation="MYRFVPVSLLQAHYGATGAIYAALYVIYGISLGFALFLVWQQYE
                     VARLTVEAEAAAVQ"
     gene            27965..28915
                     /locus_tag="VN178_13950"
     CDS             27965..28915
                     /locus_tag="VN178_13950"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HWS82117.1"
                     /translation="MARRTLYLRTLLGTLTAAVVVACAVALLVVSEEAEATFPGKNGR
                     IAFSSYDGNDYEIYTVNSRGGDKFQVTDNARDDYSPSYSPNGKRIVYVELDRNDAELY
                     TINVGGRGKTQLTHNNADEAAPSYSPDGKKIVYMVYSNASGVTEIYTIYIGGGGKTKL
                     TTGGQPSYSPDGKRIVYWDYHKTAKNPHPDTEIYTINASGGDETQLTHNNSDEFAPDF
                     SPDGKRIVFAGLDGLERNNAESDIYKMKVGGGAKTRLTNNNTYDTYPSYSPDGKKIAF
                     AVSKGSAGGDIYTINAFGGGKTQLTNDNSDEAAPSWGSRQ"
     gene            29391..31832
                     /gene="leuS"
                     /locus_tag="VN178_13955"
     CDS             29391..31832
                     /gene="leuS"
                     /locus_tag="VN178_13955"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWS82118.1"
                     /translation="MSETKQRRTYDPTDLERRWQERWAESGLYKTDEDESKPKHYALT
                     MLPYPSGDLHVGHWYPMTPSDTRARFMRMNGYRVFFPIGFDAFGLPAENAAISRGIQP
                     REWTYANIERMRGQLRQMGAMFDFDSQVVTCDPEYYRWNQWFFLKFFEKGLAYRMEAP
                     VDWCPRDNTTLAREQVVGPDRRCERCGTPVIKKNLPQWLFKITNYAEELLDFSRIDWP
                     ERVETLQRNWIGRSEGAAIRFEIEGYGPVEVFTTRPDTLFGATFFVISPEHPAVGQIT
                     TPERADEVRAYVERAARMSEIDRTDVTREKSGVFTGAYATNPANGEEIPVYVADYVLM
                     GYGTGAIMAVPAHDERDLAFAKKYGIEVRTVIAPPDWNGEDLVEAYAGEGTMVDSGRF
                     DGTPSTEGKENVTAWLEERGVGKATVTYRLHDWLISRQRYWGTPIPIIFCDECGIVPV
                     PEEDLPVLLPDESEFMPTGESPLKLDPTFYNTECPGCGGPATRETDTMDTFMDSSWYW
                     YRYLSPHYDEGPFDPERGEKWLPVDQYTGGIEHAILHLLYARFFTKVMRDLGLVDFGE
                     PMTRLRNQGSILGPDGQKMSKSRGNVVNPQEYVDRFGSDALRGFLMFIGPWNEGGPWD
                     GKGIEGVSRWLRRALSLVTGGDSSGGEADPTELERRTNRLIKKVTDDLDAFRFNTSIA
                     ALMEHTNYLLAVRGRVGNDEWHEALRAFVTVLSPFAPHHAEELWAEMGEEYSVHEQAW
                     PGYEEALIQAPEITLVVQVNGKLRDRIDAPADISEEAARELALSSARVRPHLEGKELR
                     KSIYVPGRLVNLVVG"
     gene            32131..33474
                     /locus_tag="VN178_13960"
     CDS             32131..33474
                     /locus_tag="VN178_13960"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HWS82119.1"
                     /translation="MVKKARDTARGYIFVAPKNGPDEAGPGQDGCMILDNDGQPVWMR
                     LLQNEDMDVMAFRAQTYKGERVLTWWEGLHTGYGQGHYVILDSSYRQIARFGAQNPYQ
                     GDHHEFLITPHDTALITIYHKVAMDLSGVGGPVDGEVLEGIVQEIDIQSGEALFEWHS
                     LQHVGLDESLYAPPADLEAPFDYFHINSIDVYDEDHLLISARRTSAVYKVDKRTGEVV
                     WRLGGEKSDFEMGHGTRTDWQHDARHQGDGTITIFDNGGVEKDVQSRGIVVEIDEDAM
                     SATLVGEYTHPNKILAATQGNLQVLPNGNVFVGWGSEPSYSEFGPDGKLLYDASFPSQ
                     VESYRAFRFPWKGQPKDRPAVVAEAGPDDRVTVYVSWNGATEVTAWQILAGGSPDELE
                     PAGSARRRGFETVVTLQTGEPYVAVEAKDGSGRVLGTSEAVEPVGHSASTGQTRT"
     gene            33496..35685
                     /locus_tag="VN178_13965"
     CDS             33496..35685
                     /locus_tag="VN178_13965"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HWS82120.1"
                     /translation="MDKRRIQLRWFEAGAALAVIAAFAVIGPLRGTFAPIPWVMLLCT
                     LILFLTPGALLVRWFWRDYFFGAALAPAAFVTSVGLFALLAVPMLILQGTLDAYLWAC
                     GTIVAACWLAAVVLAFRPEQAEEAQSGFSLSDRGGMLWMPFAALVAALSYIARNTAPS
                     YYGDIWIYLSWVREYLGGGRLASVEPFFGSEVGLSRARINGWILEQVAVSRVSGVDPI
                     ELAFSYLNPALVVVAFLVFYALARILFESEKAALFSGCLYALFFFIHLSQSRIAWGGE
                     FVQRLPEDKLVAKFLFLPLALAFAYAFMQGGAKRYFIGFSFLCCAVMAVHPIGLAIIG
                     ISMAGFSVLHLAADPRRRAAWIRISAMGLTGVAIIAVPAVLVSSFTDESLSNALADSD
                     INSGDPDVLRNMIFVSPERNRIFELADGSYIMHPSLLLDPVIAAAFLIGLPFLLWRLK
                     RSLAAQLLFGTMYVTTIIVYVPPIATFLGDNVVLPGQIWRLAWPVQLAAVLTLGWLVW
                     GATDLVAAWLRSFRPARLLAGALPILLVIALAAAAVPQARQGMESIQAHREASRESGF
                     YPSDPIFPWFREEIRSPVVVLAPDIQSARIPAYSSEANVVSRRGGLVLRVLPELKERA
                     PGQIEVPQGSLDVQEFFSGATLQQDIEILRRNDVDYVMVARDSQLAVSLERLSGFTPV
                     ETPSERYDLYAVDLQEVASESGALPSSPGAHSVEWPSPDTRPGRLIS"
     gene            35682..36365
                     /gene="rpe"
                     /locus_tag="VN178_13970"
     CDS             35682..36365
                     /gene="rpe"
                     /locus_tag="VN178_13970"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWS82121.1"
                     /translation="MTFFGRLGDRVVGAPSILAADFSELAEEVRRAERGGAALVHFDV
                     MDNHFVPNLTVGPAVAASLAAAVNLPIDAHLQVTNPDNLIPSFIEAGVVSISVQPEVV
                     THLHRTLDLIRTGGREAGVALNPTTPPESIEWALPYVSYVNVMSVNPGFGGQAFIPEM
                     VEKVRRLKEMTDAPVEVDGGITVETAPLMVRAGAQVLVAGSAVFKGDPATEMRKIIEA
                     GQGASQHLG"
     regulatory      36449..36625
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            36627..37745
                     /gene="ribD"
                     /locus_tag="VN178_13975"
     CDS             36627..37745
                     /gene="ribD"
                     /locus_tag="VN178_13975"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWS82122.1"
                     /translation="MQQDSMMDLARCLAERGRYSVAPNPLVGAVVARDGSVLSEGWHS
                     RAGEDHAEIVALNGAGGTARGHTLYVTLEPCNHYGRTPPCVEAILRSGISRVVVGHLD
                     PNPKMRGRSVGILRDAGVEVEVAADPVFEWQNEQYFHHMRTGKPFVHIKLAATLDGRI
                     AAAGGDSKWVTGEAARRRAHELRAEAGAVLVGANTVRTDDPMLTARGLPVPSPLITRV
                     VLDPHLTISPESELARSAGEASVLVFASQAALDGRETKLERVGVEVAAAPPSEEGLDL
                     LHVLGELGERGIKGLLVEGGGETATRFVRRGLADKLTLFYAPKLLGSEGVPMIGALRA
                     TKVAESLRFSVSEVEKVGDDIAVTLYPVGEEERVHRAC"
     gene            37726..38361
                     /locus_tag="VN178_13980"
     CDS             37726..38361
                     /locus_tag="VN178_13980"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWS82123.1"
                     /translation="MFTGLVEEVGRVSSIGEGEMLRLSISAGRASEGASAGDSVCVNG
                     ACLTIGEADGETLTFFAMPETLRRTALGGLVVGSLVNLERAMSAGSRFGGHIVQGHVD
                     GVGEVLGVRPEGDAEIWEFAAPKAVLRYCVEKGSICVDGISLTIVSIGANAFTVSILP
                     QTRANTNLRELGTGGRVNLEADVIGKYVERLLGPRLAGRLSTSERSVADAF"
     gene            38351..39553
                     /locus_tag="VN178_13985"
     CDS             38351..39553
                     /locus_tag="VN178_13985"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWS82124.1"
                     /translation="MPFSPIEEIVEDIKHGKMVIVCDDEDRENEGDLTMAAELVTADD
                     INFMASHGRGLICLPMAGEIVDRLDIPQMVTHNASRMGTAFTASIEAKEGITTGISAA
                     DRAHTCRVAVDEATGPEDLVMPGHVFPLRAKDGGVLQRAGQTEAAVDLARLAGFKPAG
                     VICEIMKEDGTMARVPDLERFSKEHGVKLVTVAQIIEYRHAFETQVRCAVETRLPTPF
                     GEFRLRAYENDIDELTHVALVMGEPEGKDDVLVRVHSACLTGDALHSLRCDCGEQLEA
                     AMGRIAEEGEGVIAYMQQEGRGIGLLNKMRAYHLQDEGMDTVEANQKLGLAPDLRDYG
                     VGAQILKDLDLKRIRLLTNNLTKVVGLRGFGLEITERVPIEMEPNGHNERYLKTKREK
                     LNHVFEKF"
     gene            39563..40075
                     /gene="ribE"
                     /locus_tag="VN178_13990"
     CDS             39563..40075
                     /gene="ribE"
                     /locus_tag="VN178_13990"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWS82125.1"
                     /translation="MEGANHIEGNLSAEGSSFGIVASRFNDFVVKALLEGAIEAIRRH
                     GGDAEAVDVVWVPGSYEIPLVARELALSGRYDAVICLGAVIRGATAHFDYVAGGAAGG
                     ISNVALESGVPVIFGVITTESIEQAIERAGTKMGNKGFEAAVSAMEMADLMAKLRERA
                     SQRDTPLENP"
BASE COUNT         7494 a        12339 c        12698 g         7550 t
ORIGIN      
        1 cggtcgacac cgaaacggcg ggcggtctcg gccttgggga cacccttctt gaccgactcc
       61 acgatcttct gcctgagatc tagcgaatat gcactcatgc atccacgata cccggtgcac
      121 cgtcatcgca atccgctgtc agcacgctca ggcttttagc cttcgcttgt cagcatgtca
      181 gcgaagcgct tcgtaacctt gccatgagcg ccgtacctga ctgccgttgg cagcggttcg
      241 tcatgcgcgg cagggcgagg ccggaaaggg agaagctgac ctgctgacaa gctagtccgc
      301 aggccgaagc gtgctgacta gctgaaatgc tgaagggcgc tcttgacctc ttgcttttcc
      361 gcgttcaggg cgtagaaggt catgtcggcc ttcgcctgtt tgtagggctt ctggcgcacc
      421 ttgcccaggt aatccgcgtc gagcatggcg gttatgacgt ccccgatctc ttcccgcgag
      481 agctccagtt cgccgcgctc atcgagatct cggatgctgg agaagatctc caccaaggtg
      541 gcgggctgtt cgagggaggc gacgctcgtg cagaccgtct tgagctcgta gggcgtgagg
      601 tcttcgagga gatcgaagcc tggttctggc tctacttgct ggcgctcttc acgcgcgggc
      661 ctgccgttgc ccctgttccg gtttgcgggt tggctctgtg atgtgggctc tacggctgcg
      721 aggtcggtct cctgcaggtc gggaacctgc tcgtcgggca ggtagacctg gagctcgagg
      781 tcctggtcaa cggtgtttac caggcctcgg cgctctgcct cgaccacgaa gtccttgaac
      841 ttcttgaagc ccgcgttccg ctcgtcgaaa gagccgagct gcacgatcat ctgctgcttc
      901 acctgcccga ggacccgtgt ctttccgccc ttcttcagcg cttggacggc cctgaccagc
      961 tccttgtagg ggtccgcggc ctcctttttg ggttccctgt cctgggcgcg ggcggcgcgg
     1021 tcttcttcga gcagggaggc gtaggagacg aagtggtctg cggattcgat caggtggtac
     1081 gaagtcgcct cggagacccc gattaccacc acgttcttcc ccttgctcct gagcaggctg
     1141 acgagcggga tgaagtcccc atcgccggtg acgaggacgt aggtgtccac ctgcgggtgg
     1201 tcgtgcaaga aggccgagca ctcgaccgcg agcatcacgt ctatggagtt ggtgcgacgc
     1261 ttctgagact tgcctccggg gaagtagcgg gccgaaatgt agcgcggcga gatgccgttg
     1321 gaatagaggg tcggcggggc ctgtcggaag tctccgtcgg tccagtcggc gtaagccgag
     1381 gccgcgacga tgcgtccgta ctcacgggcc ttctccagta tggccgagac gttcggcttt
     1441 gatttgtact ccgtgaccgt agagatgtag atgttctccc agtcgataaa tactgccaga
     1501 tcttcaggca tgaattgggt tcctaacgta gaggagaggc ccggatcccc gggcctctgt
     1561 tggtctctta tacactgtag cggtcgtgga tcctcaccgc ggtctgggcg agggtctcct
     1621 cgtcgagaac ggcttcgatc cagagtagtc gttcccccac ttcgaagcct tctggtaagt
     1681 cgaaggctgc gactttcgtg acggggtctt ccgttccgtc gaggtagacg gagtactccg
     1741 ccgactccat aaggacctct ccgtcctcgt cggtgacgag gacgcgggcc cggcgctccc
     1801 tgtccgagac gtgttcgcag ccctcgaact tgaagtagac gacgaacgat gtcccaccgg
     1861 cgccgttggg gcggatctca tcgagcaccc cgaagagcgt cagcctacct tcgtactcag
     1921 tacaactctc ggcgagcacg agggccgtgc actcgaccac gccttcgcac tcgctatgca
     1981 cccggcaccg tcccgttccc gtcggcttcg ggaagacccg cggtagaggt gcccgtctgg
     2041 tcctccccgg agagctcatc cagcgtcagc tcgtccgtgg cgccgtcggc gtcggcggat
     2101 cgggaggaga tgactttggc tatggcgctc accgagtcgc cgctcctcag gttcatcacc
     2161 ctgacaccct gcgccgcgcg gccctgtcgc gagacagagt cggcatccat gcgtatggtc
     2221 gtcccgaagt tggagacgat catgagctcg tgtagtccgg ggcggacgac ccgcacgccg
     2281 gcgagtttgc cgcgttctcc gtcggtcttc atggcgatga ctccctggcc gccgcgtccc
     2341 tgggccctgt actgcgagag ggccgtgcgc ttgccgaaac cgcgctcggt gagtatgaac
     2401 aggtccgccg catcgtcgga gatcacgtcc atagagagca cgacgtcgtc tttgccgagc
     2461 gtgatgccct tcaccccggc cgtagcgcgt cccatcggtc gggcgtcggt ctgcaggaag
     2521 cggatgccct tcccgttgcg gctcacgata acgacctcat cgccctccga ggcgtagcgg
     2581 acggagatca actcgtcgtc gccggcgagg ttgatcgcga tgatcccgtc gttcttcaag
     2641 ggcgtgttgt actcctggaa cctggtcttc ttgacgatgc ccctcttggt cgcgaagagc
     2701 aggtactcgg cctcctcgaa ttccttggtc gccaggacgg aggcgatgtg ctcgccctgc
     2761 gcgaggggga gcatgttggc gatgtggcgt cccttcgcag cccggcccat tcgcgggagc
     2821 tggtggacct tgagcctgta gactttgccc ttgttggtga agaagagcat gtagtggtgc
     2881 gtcgtcgtta taaagaggtg gtcgatgtag tcaccctctt tgagctccat cccggcgacg
     2941 ccgaccccgc cgcggccctg cttgcggaag gagttgacgg ggacgctctt caggtaaccg
     3001 ccgttggaga tggagatcac catctcctcc tcggctatta ggtcctctat ctcgaagtcg
     3061 acgccctcct cggccgtgat cgcggtcctg cgatcgtccg cgtaggccgc cttgacctcg
     3121 ccgagctcct ccttgacgat cccgtagacc ttggactcat cggccaggat gccttcgagg
     3181 tactcgatct tctcgcgcag gtccctgtgc tcctgctgga ccttctgacg ctccatggca
     3241 gttagcctct gcagcctgag gtcgaggatc gcctgcgcct ggcgctcgga gagcccgaac
     3301 cccttcatca ggttcttgcg cgccgtctcc acgctgcgcg agcggcggat ggtcgccacg
     3361 acctcgtcga ggtctccgag cgcgatcagc agtccttcga ggatgtgcgc ccttgccttc
     3421 gcccgcccga gctcgtagcg cgtcctgcgc gttaccacct cgaactggtg gacgatgtag
     3481 tgcttcaggg cctgcctgta ggagagcgtc ctcggcacgc cgtcgacgag cgccaccagg
     3541 ttcaccccga agctctgctg catctgggtg tgcttgtaga ggttgttgag gacgaccttg
     3601 ggcacggcct cgcgcttgag ctctatcact attcgcatgc cgttgcggtc cgactcgtcg
     3661 cgcaaatcgg agatgtcctg cagcttgcga tccttgacca gctcggcgat cttctggagc
     3721 agatagctct tgttgacctg gtaggggatc tcagagacca cgatctgggt cctgttgccc
     3781 tttatctgct cggtgtgggc cttggcctgc acccgcacgg agcctcgtcc cgtcgcgatt
     3841 gcgtcgcgga tgccgcgcaa cccgacgata acaccacccg tcgggaaatc gggccccttg
     3901 atgtgctctg cgatcccctc gtcgtccagc tccgggtcgt ctatcagggc tatcgtcgcg
     3961 tcgacgacct cgccgaggtt gtggggtgga atcttggtgg ccataccgac tgcgatgccg
     4021 tcggatccgt tgacgagcag gttcgggaac cgcgccggca agacctcggg ctgtttcagg
     4081 ctctcgtcga agttcggaac gaagtccacc gtgtcggcat ttatgtccct cagcatctcg
     4141 gtcgccatgc gtgtgaggcg ggcctctgtg tacctcatcg cccccggcgg atcgccgtcc
     4201 acgctcccga agttcccctg gccgtccacg agcgggtacc tcagcgcgaa gtcctgtgcc
     4261 atgcgcgcca gcgcgtcgta gatggcctgg tccccatgcg gatggtactt acccatgacg
     4321 tctccgacga ccgtcgcgct cttgcggtat ggcctgttgg gctgcaaccc gagatcctgc
     4381 atcgcgtaga gaacccgccg gtgtacgggc ttcaggccgt cgcgcgcatc aggtaaggcg
     4441 cgcgagacga tcacgctcat ggcgtaggaa aggaaagagt cctttaactc atcttctatg
     4501 gagtggggct gtacgttgcc cgagaaagta tcaagcatcc aggttcttca cctctctggc
     4561 gttctcctct ataaactgtt tgcgcggctc gaccttgtcg cccatcagcg cggtgaacag
     4621 ctcatccgcc accgccgccg actcgaccgt gacctggagc atcgtcctgt tctgcgggtc
     4681 catcgtcgtc tcccagagct gctgcgggtt catctccgag aggcccttga agcgggagat
     4741 acttgcgttt ccgttggccc tgatacggtc cagcgtctcc cgcaactcgg tatcgttctt
     4801 ggcgtagtgc tcgcggcggt tgtgcgtgat cttgtagagc ggggggaggg cgatgtagac
     4861 gtagccggcc tcgatcaggt ctcgcatgtt gcgatacaag aacgtcagca ccaacgtccg
     4921 aatatgactt ccgtccacgt cggcgtcggc cataatgacg atcttgttgt accgagcctg
     4981 ctcgagatcg aagctctccc ccacaccggc gcctatcgcg cttatcaggg cctgtatctc
     5041 ggcgttggag aggatcttgt tgatgttggc cttctccacg ttgaggatct tgcccctgag
     5101 cggaagtatg gcctggaagt ttctgtcccg cccgcctttg gccgatccgc cggccgagtt
     5161 gccctcgacg atgtaaagct cgctcctggc agggtccttg gatgagcaat cggcgagctt
     5221 gccaggcagc gtcgagctct ccaggtagcc cttgcggatc ttctccctca ccgtcctggc
     5281 cgcgagccgg gcccgcgcag cctgcaacgc cttgttgacg atcgccttgg cctcaccagg
     5341 acgctcctcc aagaactctg caagataacg gttggtcacg ctctccacga gacccttgac
     5401 ctccgtgttc ccgagcttgg tcttggtctg accctcgaac tggggctccc gcaacttcac
     5461 cgagatgacc gcggccaacc cttccctgat gtcgtcgccg gtaaggctct cctctttctc
     5521 cttgaggaga cccttctgcc tggcgtaggc gttgatcgtc cgcgatagcg ccgacctgaa
     5581 ccctgagagg tgcgccccgc cttcgtgggt gttgatgttg ttggcgaacg tgaagacgga
     5641 gtcctggaag ccgctgttcc actgcatggc gacctccacg tcccccacct cctcctcgcg
     5701 ctcgaggtag aagatggtct tgtggacggg gtccttcgcc tcgttgatgt gctccacgaa
     5761 gtcccttatg ccgccctcat actggtacgt gacatccctg tcttcttccc gctcgtctat
     5821 gagccggatc ttgagtccct tgttaaggaa agcccactcc cgaaaccagc ggctcagggt
     5881 atcgaaggag aagtcttggg tctcggtaaa cacctcgggg tcgggcttga agctcgtggt
     5941 cgtccccgtc ccctcgccct tcttgagctt acggcccttt ttgaggtcgc ctttcgggaa
     6001 cccgcgctcg tagcgctggc tccacacgtg cccgtcgacc ttgatctcca actcgagcca
     6061 ttcggagagc gcgttcacca ccgaggcgcc gacgccgtgc aacccgccgg agaccttgta
     6121 accttctcca ccgaacttcc cgccggcgtg caaggtcgtc atgatgatct cggcggccgg
     6181 cttgccgtac ttcgccattt tgcctaccgg cataccgcgc ccgtcgtcgg cgaccgtgac
     6241 ggagttgtcg gggtggatgg tgacgagtac ctccgtacag tgtccggcca gcgcctcgtc
     6301 tatggaattg tcgatgatct cccggacgag gtggtgaaga cccctcggcc ccgtcgatcc
     6361 gatgtacata ccaggacgcc tcttgacggc ttcgaggcct tcgaggacct ggatcgaact
     6421 tgcatcgtaa gcgctcttgg cattcttggt catggactta gccgccaaag aaccggaccc
     6481 tccttttggt cagtccggcg aaggcgccgg agtcgttctt ttcatcgtaa ctctgcaccc
     6541 gatgattgta acacattcgt tgccgtttcc aaacacgatc tcggcccatt tgcggcttat
     6601 ctaagccaat tccgcagggt agcgctacct gctggcacca tccgttgcgg tctccaggat
     6661 ggaaccacca gatatgcgta tgatacgggc tctacgcaag atttcctcgt cgaaatattc
     6721 gaggttggtc gtcgagatca aggcctgcgt gctctccaga aaatacccgg ccagatactc
     6781 tctgcgccgc tcgtcgagct cgctcatcac gtcgtcgaag agcaggatcg ggtcctcccc
     6841 cacctcccct cgcacgtagt ccctcgaggc gaacttcagc gccagcgtcg cgatcctctg
     6901 ctgcccctgg gacccgaaag tggtcaggtt caccccaccg agcgagacct cgaagtcgtc
     6961 cctgtggggc ccgacagaag tggtcccccg ttcgacgtcg gcgctgtgcg cctctctgag
     7021 cgcctcggaa tactcttcga gggtcgagct atggctgtag cgtatcgcgg ccttctcggg
     7081 gccgtacagg gcccttagag cgtctgtgaa atgttcttcg aggacgggca gagccgcctc
     7141 gcggccggcg aggacggtct ttccgagttc gacgaccttc ttgtcccacg tcgcgagcgt
     7201 cctctcggaa gagaacccgt ccctgatgcg tctcaggagt tggttgcgct gctggactgc
     7261 cttggcgtat tcgagggcgg cgcgcgcgta tgcgggacgt agagaggaga gcagggcgtc
     7321 caggaacgcc ctgcggtctg cgggcgcgcc tttcactacc cttatgtcgt cggggaagaa
     7381 cgtgacgacg cgggcaccgg caccgccggc ggcgaagttg gtgagggaag gggccggtac
     7441 cccatcgacg gtgatgcgct tcttctgtcc cggggcatag ccgatggcca cgccacgttc
     7501 gccaggggag ccgtcgagcg cgactctggc ctcggtgcgg gcgaattcag cgccccagcg
     7561 gacgacctcg ttctccttcg gggtgcgggg cgaggagccc gtgacgttga aggccacggc
     7621 ctccagcagg ttcgtctttc cctgggcgtt atcacccacg accacgttca gcccgggggc
     7681 gagcagggcc gtggcgtcga cgtagttgcg gaagttgacc aggcgcaagg cccgcagata
     7741 ggcgctcaac cgatctccaa ccgctcgtcc cccacctcga ttacgtcacc ctcctggagg
     7801 gtgcgaccgc ggcgcgtctc gacctcaccg ttcacgctga cctctccggc ctggatcagg
     7861 atcttcgcct caccgcccgt cccgacgaga ccggcagcct tcagcgcccg gcccagcgta
     7921 gtgcccggtg ggagtcgcaa cggtcagctg cgggacgggt cgcgcatcgg catgatcaaa
     7981 tacaggaagt cgggctcatc accgttctcc tcgggcacga tgagccctgg cttgagcggt
     8041 tcgttgagct tgaagaccac gttctccgaa tctatcgcgg agacgccgtc cagcaggtag
     8101 cccgggttga aggagatcaa aaactcgtcc tcgctggagg cggagagcct ctcgtgcgcg
     8161 tctccgacct cgccgttgcg cacgatcacc tctacggaac cttccgagaa ggagaggcta
     8221 acgggcaccg gcggcgtctg gcgggcggcg aagaggttga ccctcctcag ggtgccgacc
     8281 agctcctccc tggagacgga gatctcacgc tcgaacccgc cgggcaagag tcgcctgtac
     8341 tcggggaagt tgccgtctat gagccgggtg cccaaaacga cgtcgccgat cctgaacaac
     8401 gcctggttct cggagagcgc cacctcgacg tcttcctcat cggagccgga gaagatcctg
     8461 gagacctcct gcatcgcccg cgccggtatg atcgcctccc tggaaccttc gaacgaggtg
     8521 gtcgcgagct ccgtctcctt gatgctcagg cggtaggagt cggtcgtgac catccttacg
     8581 cgcgagtcct cgaagctgat caggatcccg gtgagcacag gcctggtctc gtcccgcgag
     8641 tacgaccgcg acactttctc cacggtctcc acgagcgacg acccggacat cctgaaggta
     8701 ccctcctccg ggaaggcggg aagccgcgga tagtcgtcgg ccgcgtaggc ccgaatgcgg
     8761 tactcgttct ccccggcaac gagcctgacc gtcccctcgg agtcgtcgtg ctccagcgag
     8821 aagctcccgc cgggcagcga ccttacgatg tcgttgaaga tgcgggccgg gatcaccacc
     8881 cgcccttctc cttcgacctc ggccacagac gacgtctgga tcgagatctc catgtccgtc
     8941 gccgatagcc ccagcacctc tcccctaccc tcgagaagta tccccccaag caactggatg
     9001 gtcgacctcg cagagacccc gcgcgagacc agagccaact ttttgccaaa cgtttccgtg
     9061 ctacaaactg ccctcatgca gcctcccttc tcaccttatt attattacct aaccatatat
     9121 ctattagaac aataataata ataaggcctg tggatatgtg tataactcga aaaaatcgct
     9181 ttacctatgc cgaatagtcg tcctcgggcc tgtgggcaaa aatgtggata actcgcgatt
     9241 tcctgtggac ggattgtgga taagtgccga gttatccaca ggccagagtt ttccacaatt
     9301 ctatccacag gtttatccac agattatacc cctcttatcc acaggtttat ccacaggtat
     9361 ccttacgatc aaacgttaca atctgcctaa attcctcttt caccgccgtt tttatccaca
     9421 gtttggaaga gttatccaca atttctgtgg ataagtgaac catcctgtgg aaggtttctg
     9481 gcgacttatc cacaggccgg cggcgccctg tggatggctt gtggatagaa tacgcgttct
     9541 atgcatattt cgtgttacaa tctcctgtcc ctgggggagg ggggtcaggg tcatcttttg
     9601 ctcttgatct ggtgggtgat ctcatgtatc tgggagaaga cgtcccggtc gctgttgatg
     9661 aggttggcga tcttggctgt ggcgtgcata acggtagagt ggtcgcgccc tccgaaggcc
     9721 ttccctatct tggggagtga ctcgtctgtg agctcccggg agaggtacat ggcgacctgc
     9781 ctggggtagg cgaaaccttt agagcggctc tggccgacga ggtctgcctt ggcgacaccg
     9841 aagtagcggc agacctcgtg ctggatgagt tctatgggga tctcacggta ggcctggtcg
     9901 ggtaggatgt ccctcaggac ttcttccgcg aggtcggcgc tgacctgccg gccgtagagc
     9961 gaggcgtagg ccaggatacg tacgagggct ccttcgagct ccctgatgtt cgtggagacc
    10021 ttcgaggcga taaaggtgag cacatcgtcg tcgacctcga ggtgatccat aagggctttc
    10081 ttgcgcagga tggcgatcct ggtctccagg tctggcggct ggatgtccgt cacgaggccc
    10141 cactcgaagc ggctcaccag acggttctcg agcgtctgga tgtacctggg gtgtctgtcc
    10201 gaggctatga ctatttgctt gttctcttcg tagagcgcgt tgaaggtgtg gaagaaggct
    10261 tcctgggtct cgaccttgtt ctccagaaac tggatgtcgt cgaccaggag aacgtcgttc
    10321 tcgcggtagc gcttctggaa ctcgaggggc gcgttgtccc tcatggagtt tataaagtcg
    10381 ttcgtgaact gctcgcaggt aacgtaccgg atcctcatgc tagggtcctg gtcctctacg
    10441 tagtgtccca cggcgcgcaa gagatgggtc tttcccaggc cgacgccgcc gtagataaag
    10501 agtgggttgt agacgaggcc gggggtctcc gcgacggcga gcgtagccgc gtgggcgaac
    10561 ctgttgccgg cgcctatgac gaacgtgtcg aaggtgtatt tggcccggaa ggatctcggc
    10621 gtacgtacgt tgagtacgga ctcggccccc tcggcggccg taccgttccc ggtatggacg
    10681 cctggtccac cgacgctgac cacgagggag gtctcctggc ccaccacgtc gtcgagtacc
    10741 tcctccagca gcggcctgaa gcggctctcg atgtactcct tggcgaacgt gttgggaacc
    10801 gatatctcca gctgatcgtc gtagagggcc acgggacgta tgccctcgaa ccagacccgt
    10861 aaagacgaga cgtctatatg ctcggaggcc cgatccagga cctccgtcca tacctgctct
    10921 gccttacccg ccacgcttac ttgacccagg aattgattat ggccccgacc agatccctgc
    10981 ccaagggaga gatgaggcgc ttgcccgact catcggccat gacaagcccg tgctcttcga
    11041 gtacggagag atccctgtac gccgtgctca cgcttatctc cagcctgtct gccacggtcg
    11101 agggccccac ctcgccgcac tcgagtacgg tgatgagcgc cttctgttgc ctctcggaga
    11161 tgttcatccg tggtactccg tcggggtacc gtctctgcgg gggtggcgga gcaacctcct
    11221 tcgaggcggc ctcgtcgccg gccacggaga tggttgccac ggtacctcca ccgatgttgt
    11281 cctcgatggt gagggtcccc cctgacttct cggccgccgc gcgtgcgatg ccaagcccgg
    11341 ctccgacgcc ccggatctca ccaagggctt ccggcgcggc gccgctgaaa ccgaactcca
    11401 tcgccctgct cttgttctcc accccaggac ccttgtccga gacgcgtacg acgttgccgt
    11461 cgtcgagaac ggagatgact acaccctggt atccggcgtg tatcaggttc tctaccacct
    11521 cccgaatcga ctcctctgga actctgcccc cagcgtctct caccttctca gtcacgaacc
    11581 tggtgaactt ccgcgttgcg gaagccggat cggacgccga aacctccgtg atctccggcg
    11641 tacctccccc gggatagact gctatgcgaa cgcctacgcc cgcctgctcc gtctcccctg
    11701 atgtccgctg tgcacgcatc atccccagat aggaatttag cgcacccccg atcacagcgc
    11761 tacagcccag gatcgtaaaa cctgcgtttg caggtaatta acatggttat ccacagattt
    11821 atccacaggc tgtggataac taaggaggaa ttcggcctgc ggagcgaccg taaggccgtc
    11881 aagggagcac tttagccctt gtgcggagat gaacgacccg gagcacctac gcatcgtaaa
    11941 cgccgttttg cagcattttt ctgatagtgg ggccgggatg tctcgccttc gcaaggacgg
    12001 actcaccgtg gagattgctg gatggtgttg caagcttcgg atatactctc gtcggcggat
    12061 ctaatacttc ggagtgcctg tagaggagtt ggatgaagcg tacttatcag ccaaatcgtc
    12121 gtaagagggc caagacgcac ggcttccgca aacgcatgag ctcggtgggc ggacgcaggg
    12181 tcgtcgctaa ccgtaggcgc cggggccgca agcgtctggc agtctgatcg ttggcccgta
    12241 gcaagcgaat gcgtctgacc gactcgcccg agttcgagcg ggtctaccgt cagggcaccg
    12301 cttacagggg caggctcttc tccgttcacg cctttccaaa cgagcgtggc actccgcggc
    12361 tagggctctc cgtctcccgc aaggtcggca acgcggtaac gagaaacgcc gtgcgaagga
    12421 ggctaagaga ggtgttccac tcgtgcatct cgggggcatc cgagaacctc gacctcgtgg
    12481 tgtcggcgag acccgctgcg gctgaggcta cgttcgagga gctgcgggag gagttcggca
    12541 agtcgttggg tagggtcggc ggcccggtcg ggcgccggcc ggcttgaccg tagctgacca
    12601 tgaccgcgag aagggcgagc acgggcctta tccgactcta ccgcaggtac ctctccccca
    12661 tgctaccccc atcctgcaga tttacgccga gttgctctct ttacacgctg caggccatag
    12721 agaagtacgg cgtaccgaag ggcatcttca tgggcgtcct cagggtgctc cgttgtcacc
    12781 cattctcgaa gggaggattc gacccggtaa gatgagcagt atctcatgat caacgcgata
    12841 gcagacttct tccagaacct gttcagtccc atagtgagcg tcctcggcgc cgtgctgctg
    12901 tacttccatc agacgctcgg cgcgccttgg tggctcgcca tcgtgctgct gacggtgatc
    12961 gtgcgtagcc tcctcttccc cctgactata aagcaggtca agagcatgcg cgccatgcag
    13021 gatctgaaac cgcacatgga tagggtccga gcccaattca aggacaatcc ccagcgccaa
    13081 agggaagaga tggcgaaggt ctaccaggag cagggggtca atcctctcgg aggttgcctc
    13141 ccgatcctgg tccagatgcc gatatttatc gggatcttct acgttatccg ccagttcggt
    13201 ggttacagcc tcggcggcag gacggtcgag gcgcaatatc ctagcttcca cgaaggcggc
    13261 atcctgtggt tccaaaacct ctcgattgcc gatcccacgt acctgctccc cataatctcg
    13321 gcggtaacca tgctggcggc caccgagata accgcgaaga atatcgaccc ccagcagcgc
    13381 tggctgatgc gcctgcttcc catcggcttc actatattcc tcctcaactt cccggccggg
    13441 ctgttcgtgt actggatcac ctcgaacctg gtaaccctcg gtcagaactt cgtgatctat
    13501 aactacggtc ctggtaagaa gtcaaccgat gttgcgaagg ccaccgatgg cgtaaagtcg
    13561 aaatccaaga acgggcagac gcggaattct gacgatggac caccccccga acgcagtcaa
    13621 gaggagatcg ctaaacaggc ggcgaaggtc gcgaaaagga agcgtagaaa gaagaagaaa
    13681 tgacggacag tgtggagttt tatgcctcta cggtcgacga ggccgtcgag aaagccgccc
    13741 tcgaactgaa cctcccctta gcggaggttt cctaccgggt gctcgatgag ggcaactctg
    13801 gtttcctcgg catcggagcc agggacgcca gaatcgccgt ggaagtcccg gcacatcata
    13861 cgccggacga agataacgaa ccgccggaaa ccacggccgc acaggacgtg gcggtcgacg
    13921 agcctgaacc ggcacccgct gaaagcaccg ggtcctccac gctgtccgag gtctacgggg
    13981 aacaaaccga gtcggaacag atcgtggccc ccgaggagct gctggaggag atccggaccc
    14041 tcgtctcttc ggtcctggag gccatgggct tcgaagcccg tatcgaggtc tacgatgctg
    14101 gcgggttcat cgccgcagac gttgctccgg ataacacagc gctgttcata gggcaaaagg
    14161 gcgagacgat cgatgccctc caatatctcg tcaacgtgtc cgccttcaga gagcgtccct
    14221 acttcaagag gatagtgctc gatgccgaag gctaccgcca acgccgcgtc gaggcgatcc
    14281 aaggcatggc ccaccgaacg gcgcgccggg ccgtccgcga acggcggacc gtggaaatgc
    14341 cgcctatgaa ctcctctgaa cgccgcgtcg tccacctgta tctctccgag aacccagggg
    14401 ttaccacgca aagcgaaggc agcggcgata gccggcgggt caaagtctcc cccaccacct
    14461 aggtatagat ccccgtttca cgtgaaacat agtccatcat ctgcgcttga atgtgagcct
    14521 gacgacagga gtttatctgt gcaaactcct gtcgcatact tatgtttcac gtgaaacgct
    14581 ctggggatgc tcttgggctg atggaggtac aggcggcagc ctgggggctg cgtcttggcg
    14641 gcgagcggcg cgagcatctg aaggagtacg ccgggcttct tgcctgctac gaccgtgcga
    14701 acgtcatcgg cacccgggat gtagacagga tactggtgga ccacgtcctc gattcgttga
    14761 gttgcttctt gcaagaggcg ctgtttcgtg ccgggcgtct ggccgacgta gggagcggag
    14821 gcgggcttcc tggcatcccg ataaagatcg tgaggcccga ccttgctacg accctcgtgg
    14881 aatcgacagg caagaaggca gcgttcctgc ggcgtgcggt cgaagatctc agcctcaggg
    14941 acgtagaagt cgcgaatacg agggttgagg atctcggcgg cacgtcggcg catcgcggcg
    15001 cttacgacgt cgtgacgtgc agggccgtag ccagcttatc ggtggtcgcg gagtactgtg
    15061 tgccgctcct gaggaccggg ggttgtgcga tcaccatgaa gggccggttg gacccggagg
    15121 aacttgcaga aggccacggg gcgctcaagg cgctcggggc gagggtggcg gagactacga
    15181 gggtgcccct gctcccagag gtcggtgaga aggagcgcaa cctggtaatc ctcgaaaaga
    15241 ccagggagac cccgccgcga tatccgcgcc gggcgggcat tgcggccaag agacccctgg
    15301 gcattcgctg agcagcccgg acacgacgtg ctaagatgcc cgcggtgagg actgtcgtag
    15361 ccatagtgaa ccagaaaggc ggtgttggta agagcacgac cgccatcaac ctggccgctt
    15421 atctcgcagg gaagggcgaa agagtcctcg tcgtcgacat ggacccgcag ggcaatgcca
    15481 ccagtggcct tggtgtgtta ccggggcctg gcggatgcat gtacgacgtt ctgctggagg
    15541 gcaaaccctt gagggacgtg gctgtgaaga cagagatcga agggctcgac gtcgcgccgg
    15601 ccaccatcaa cctggtgggg gccgaggtcg agctggtctc cagcctggcc cgggagttca
    15661 agctcaagcg ggctttagag agactcgcat cttatgacag agtgttgctg gattgcccgc
    15721 cgtccctgga tctcttgacc ctcaacgcgc ttaccgccgc agatgaggtg ctcatcccca
    15781 tacagtgcga gtactacgcg ctcgaaggac tcacccagct catgcagagc ataaggatgg
    15841 tcagggagga attgaacccc gatctggagg tcggcggggt gctattgacg atgttcgacc
    15901 cgcgcacgaa cctggcccat caggtggccg acgaagtgcg ttcgttcttc ggagaacgcg
    15961 tcttccacac catagtcccg aggaacgtca ggttgagtga agcccccagc ttcgggatgc
    16021 ccgtggccct ctacgcgccg aagagcacag gagccgaggc gtattcggcc gtcgccgacg
    16081 aggtggtgaa tcgtgggtaa gaggggtttg ggacggggcc tctccgccct catagctacc
    16141 ggggagagcg tagggggact gaagttcgag gagttgcccg tctcggcgat ccgcccgaac
    16201 tcgcgccagc cgagaaccag cttccccgag gcagggatac gcgagctcgc cgcctccatg
    16261 agagaggtcg gaatattgca gccactggtc gtccgcccga caccaggcgg cttcgagctc
    16321 atagcgggag agagaaggct gagggcggcc aaggaagccg gcatggagag ggtgcccgtc
    16381 ctcataaggc aggccggcga ggacgagtcc atggagctgg cgctcgtaga gaacctccag
    16441 agggagaacc ttagccccct ggagaccgcc gccgcctacc aggcccttat ggacgggttc
    16501 gggcttacca gggagcggct cgcgcgaagg ctgggcaaga gcagggccgc cgtcaccaac
    16561 accctgcggc tcgtgcacct gccggagcgg atacaagtcc tcctgggcga aggaaagctc
    16621 tcagaaggtc acgcccgcgc cctgctggga ctggaaacag aagaacagat ggtccggctg
    16681 gcacaaaggg tagtggccga gaaactctcc gtaaggaaga ccgaagagct cgtccgcgaa
    16741 atgctcggta atcccggcga gactccgagc gccacgaccc ggagagatag cgcgaatccc
    16801 gacgagtttc accgagcctc acgcctcatg cgcgaagccc tcgagttgcc cgtgagggta
    16861 aagggcctga agagcggcgg aaaggtcgag atacgcttcc gccaaacaga ggaactcgaa
    16921 gccctcgtct ccctcctcac cgctcaggat gacaactgat atagaatctg aaaatacaat
    16981 tagcattgtg catataaaag aaggttccta gctagaacga ttagcgaggt attagtccat
    17041 agcgaacctc gcttcggcca agagcgaaag gctgaccagc tgaagtgctg agaagcgacg
    17101 gccgaaggct ggagcgtgct gacttgctga aatgcctaca aggaaactac cgcaacacgt
    17161 gacggtagta tagacttagc tgtctgcgct atctggaggt ttctggcgtg ggggagggga
    17221 tggcatgtcg ggaggacagc taagggtttg gtcggttctg ggcgtactgt cttttgcgct
    17281 tgttctggct ggcgctgagg ggatggcgcg ggcgcaatct gaggacgatg atgcatctgc
    17341 ggcggggtcc gcgcgggtac ctttgctgga agataaaatc agtgatcaag acgccgcatt
    17401 gcggggtatg atccaggaga tctccaccgt gggcgccgag ttggaccggg cgcaatctcg
    17461 ggtggacgat gcggggtcgc gggtcgatga tctcagacga cagaccaggc agttgaagcg
    17521 acagatagcc gaccaggatg cggccttccg cgaggcgcaa gccggctaca gggcccaggc
    17581 tcgagccgcc tacaagggcg gaagcctcga gggcctggcc gccctgcttg acggttggtt
    17641 cttcgactcc tgggagggtg ttgcagggac ggatgatccc gtggtggccg gcgtcctcct
    17701 cgatagtcga cagaatctgg ccgcgtatcg agagtccagg caaacgctca ggaacacgca
    17761 acgtcagatc gcgcagaagc gacgcgacta caatgctgcg atcgaggagc agcaggcgac
    17821 aaccaaggac cttcgcctac gcaagcaggc gctcgaggga gccatagccc gcctgagcac
    17881 caacaacgct cgtacggaaa cccgtctgca aaggctcgaa gcggcggaga gggcgcgcat
    17941 tcagaagagc gtggcggcta ccggtagggg atgggtgggc agcgagcacg agctcgaggt
    18001 ggcgcgcgaa gacatagtgg ccaccaacgt cgagcccgcc tccgaggatg cttacatgga
    18061 gctctacgag gccgcggccg cgaactacgg gttcgggccc gactggtaca tactcgcggc
    18121 cgtggggaag gtagagtccg accacggcca ggccatgggg ccgtccagcg ccggtgcgat
    18181 gggcccgatg cagttcatgc caaccacgtg ggaggcttcg ggcgtggacg gcaatggcga
    18241 cggggtcgcc aacgtaatgg atcccgaaga cgccataccc gcggccgcta gctacctcca
    18301 agacggtggg gcgccgcagg actggtacag agcgctgtac tcctacaacc acgccgactg
    18361 gtatgttaag aaggtcctcg ccgtggcgga ggcgtacagg cgcctcacaa acgacgagag
    18421 cgtcggcccg tacatctagg gtgctgtgag gaaggaccgc ggtcattcct cacagcaagg
    18481 cttcaggcaa caggcttcag gctccagtat ttcgtgcagt ttgtataaag accggcgggc
    18541 agcctttctc tagcattagc tgtcggtaag acggatgtcg ggagaaggag gaaggcatta
    18601 gcgggaatag gcgggtacgt ctgtctctct tcgccgcgtt gttggtttct ggtgtttctc
    18661 tagcgggggt cgcgggttgt ggcggggttt cgggtcggga agacggcggc ggaggaagac
    18721 cgcagggagg gacgctgacc gttctggcgg cctcttcgct gatcgatgca ttcggggagt
    18781 tggcgaaccg cttcgaagaa cagaaccccg gcgtggaggt gaggcagagc ttcgagtcca
    18841 gttcgacttt gctcgcccag atccaacagg gagcccccgc tgacgtcttc gcctcagccg
    18901 ccgaggagga gatgaatacg gcagtaaagg acggccttgt agagggcgag ccagaggtct
    18961 tcgtgaagaa ccgcgagatc gtcatggtgc cgaaggacaa cccggcgaac attcggcggt
    19021 tcagggacgt cgcgaagccc gatgtaaagc tcgtgctggc ggggaaagat gtaccggcgg
    19081 ccgaatatgc cctggagata ctgggcaaag ccgaaagcga gtacggggca gacttcgcag
    19141 aggatgtact ctccaacgtg gtctcgaggg agtccgacgt tcgggcatcc gtcaaccgtg
    19201 tggtcgtagg ggacgccgac gccaccttcg gctacgccag cgactacacc ccggacatca
    19261 gagatagggt aaaggtcgtg ccgatcccac ccgatctgaa catcgtcgct acgtacccgg
    19321 tagccgccct cgaggacgcg aagagtccgg agctcgcgaa gaagtgggtc gaactggtga
    19381 cgagcgagga ggggcagcgc gtgctccaga agtggaactt cgagccggcg gcgtgaagag
    19441 acgcggcaaa gccggttcca ctaccagact tcccagcggg agcgacacgt tcgcttccgg
    19501 actcgcggtg gcctccctcg cgctgctcgc gggcttcata agcctgcccc tgctcagcct
    19561 cgtggtctgg acggtagacc aaggagcctg gcgggcgatg gcctctcccg tggcagtaga
    19621 cgccctgctc ctgagcgtca ggaccactgc tgtgacgatg gcgatcctgc tcctggtggg
    19681 gacgccggcg gcctacgtgt tggcgcgcgc cgagttcagg gggagtcgtc tgatcaacac
    19741 cctcatagac atccccgtgg tgctaccgcc gagcgcggca ggcatcgcgc tgctactggc
    19801 cttcgggagg ctcggccttg tagggcagta cctgaacgtc ttcggcatca cactgagctt
    19861 cacgaccgtc gccgtggtga tggccgaagt cttcgtggcc gcccctttct acgtcaggca
    19921 ggccgcgacg ggtttcggcg cggtagaccg gacggtagag gaggctgcca tggtcgacgg
    19981 ggccggccga tggaggagat tcttcagggt cacggtgccc ctggccttcc cggcgctcgt
    20041 cgccggggcg ctgacggcct gggcgcgggc gctaggagag ttcggtgcga cgatcatctt
    20101 cgccgggagt ttcaggggcg ttacccagac catccctctg gccatcttcg ccgagttcca
    20161 gagagacctc gacgccgccg tggcgctctc cgtgctcgtc ctgggcttcg ctttcgccgt
    20221 gatcctggcc gtcagatacc tgaccgggag acctaccggc tagactacct ctccgccttc
    20281 ggtcaggatc atgacgtccg tcgccttgac gagggctgtg gcctgcttgc cggcttcgag
    20341 gccgagctcg tccgcgctct ccctggtgat gagggccacc aggtggttgt ccccgacctg
    20401 gagcgtcact ttggccgccg cttccccctt taccacctcg gtgatggttc ccggcagtcg
    20461 gttacgggtg ctcagttgca tctcgctctc ctctctcgat ggccagcagt ccgagttgta
    20521 gcgcaagcgc taccctgtcg ttcttcaagt ctaaaccggt cagcttctgt atgcgctcca
    20581 ggcggtacag catgctgttg cggtgcaaga acaggcggtc cgcggcctcg ctcgcgttac
    20641 cgccggccga aaaatacgtc ttcagagtca gcaccaggtc gcttcggcgc gtcctgtcgt
    20701 gctcccgcag gggttctatg agcgcccgaa agggctccag cgcagtgcct tcggcgagcg
    20761 cctgcaagag cggctctacg tccatcgccg cctttccggc cggttggaag ctacgcggac
    20821 gaatctaaca gatccctctc ccgcgtgcta aaaagggcgt atgaacgcct cccgcacgga
    20881 tatgttccgc aagctgctcc agagggaccc gacaaacccc atggtccttt attcgctcgg
    20941 caacgagctc ttcaaggagg gaagttatgg ggaggcccga gatcacctcc aacgcgcggt
    21001 ggcgaacaag ccggattact ccgtcgccta caggatgctg ggccgcgccc actacgaact
    21061 ccacgaaaac gaagaggcga agaatatctt cctgaagggc agagaggtcg cccagaacaa
    21121 cggcgacctc cagaccgtaa aggagataga cgtcttcttg cgccgcttgc acaagcgcga
    21181 ggtgggggag ggatagccct cgactctgct aacatccctg gcgtgtcgga agccagggta
    21241 ggcatcttcg tggactacga gagtccgctc gccagtaggg tggccggtga cgtctggtca
    21301 gggctatcca gggctgcgct gcaagcgggg ttcacgaaga ctacggcgca ttgggagagc
    21361 ctcagggaag cccaggttcc caaggaaggt ccccccttgc gtgtcttcgc catcggccag
    21421 gaccgcccgg acgccatcga tgccgtcgtc gctgtcggcg atcccggcgc gccgcacctc
    21481 tacggtgtga tcgtcgccgt cccgagcaaa cctagccccc tggccgggtc tctcctctac
    21541 cagggtgtgg agcgcctgac agggaccggt gtgaaggccg gcatgataga acccgccctc
    21601 ctgcacgccg ttcctgcgga gtgcgagcgc tacgcccgac ggctcctcga acggctcggg
    21661 gatccgtagg gaccgcttgg tctcccagct cctgaccgcc atcctggtaa ccggggcctt
    21721 tgcggccctc gcctacgcgc tcgggatgat caaccgcagc ggcgcgctgg gcgggcttct
    21781 ggtcgggact atgatctacg cttccctcgg gccccgcgga ttcgccatcc tcgctctgtt
    21841 cgtgatcggg ggctccctcc ttacccggct cggctacgcc agcaaagcgc gaaccggcac
    21901 cgcccaggaa cacggcgggc gccgcagcgc ccgcaacgcc ctggccaact gcgccgtcgc
    21961 caccttctgc gccatcctcg ccgccgtgac aggatccgag ccctttgtcg cagccttcgt
    22021 cgcctccctc ggcgcggcct tcgcagatac ggcagagtcc gagataggac agctcctcag
    22081 ccgcacgcca cgcctgatca ccacccttca gagagtcccg cctggcaccg acggagcggt
    22141 ctcgtttccg gggacactgg cgggtgttgg agcggccgga ctcaccgccg cgctggggct
    22201 cgtcctaggg atgctggaga caccggcagg cctcctcctc gtatcgacag cggcgttcct
    22261 gggcacgatc gttgacagcc tgatcggggc cctctcaccc cgtgccggca acgaactcac
    22321 gaacgtactc tgtacactcg tggccgccct gctcgccatc atcttcgcct gatctaccgc
    22381 ctaacccaga aacctcgccg taaacccggc ggctcatatg gggcgtcggt ccggcaggaa
    22441 cataggatca gtcggtagtc cacaaccgca tccaaacact tagatatcag ggccaaaaca
    22501 ctaaaatggt tgggctgttg agtccttgaa gggtgctcgg aacgggcgta gaggggttct
    22561 tcagcacgct ggtgaattct gtgcacttcg tcctagaaac ggccttctaa gaagttgtct
    22621 catcgttgca agggccctgt tgcccatcta aagttgggga cgccgcagct tagaacaaac
    22681 caccccacgc ccgaggagga tgacctttgt tggacgcgaa caagtacgag gtcctctcgt
    22741 tcgactgcta cgggacgctc gtggattggg agagcgggat aatctcgggc ctcaggcccg
    22801 tcttgcgcag ccacgacgtc gaggccacgg acgacgagat actcgccctg cacgcccaga
    22861 ccgagcataa gctgcaagcc gcttccgaga gggatggata cgtcaagtac cgcgacgtgc
    22921 tcggcgaaga ggtccgggag gcgggcaggg agtggggttt cgagccaagc ccttcggaag
    22981 tggaggcact ccccgactcc gtgaggtact ggctgccatt ccccgacacg gtgaacgccc
    23041 tgcgcgccct aaagggccga tacaggctag cgatcatctc caacatagac gacgggctgt
    23101 tcgccctgac agccagatac ctggaggtgg agttcgacta catagtcacc gccgagcagg
    23161 ctggcaccta caagccctcg ctgaacaact ttgagcttgc tctcgatagg atcggggtgg
    23221 cgcccgagaa gctcctgcat gtggccgaga gcctcttcca cgaccacgtg ccagcaaagg
    23281 agatgggtct cgataccgcc tggatccacc gacgagcggc caaggggggc ttcggggcta
    23341 cccctccagc ggaggccgag cctgaccttg aggtgccgga cctggagtcg ctggtccacg
    23401 ccctggggtt gagggggtcg gagggagacc gctactgatc acgctatgca cgcaactgca
    23461 ggtaagggat tctccgaagg tcgtttgcag gatgctgcat agccccggtc ctattggcta
    23521 aggagaggcc gcattgcacc ggtccggtac cccaagggct caagagagcg ctgagaggcc
    23581 gtggtagcgg aggatgaaca acccagagga gggtgtatgg aggttgtagt cgatggcagc
    23641 cctagggctt gcctaacgaa ggctttcgat cacctcaagc acgatttggc agttcggtgg
    23701 agcccgagga cttcgtcgtc gctcgaaccg cgttggggag agcgaaccat gattgagcag
    23761 cgaaggagag aacacgcgtg tcgatagaaa acccggcgct ctcggtgagc gccaccgact
    23821 accacacggc gggcgagccg ttccggatag taagcggcgg cgttgggccg atcctcggga
    23881 agaccatgct ggacaagcgc gagtgggcga tcgaaaacct cgaccgcgtg aggaggctcc
    23941 tagtctacga gccccggggc cacgcagaca tgtacggcgg cttcgtcacc gagccggacg
    24001 accaggcggc ggatctcggc gtcctgttct tccacaacgc cggtttctcc accgcctgcg
    24061 gccacggtac gatcgcgctc gccgcgtggg ccgtcgagag cggcatcgtc gtcccttccg
    24121 agcccgaaac ggagattgtt atagacgttc cgtcggggcg actgcccgtc gttgcgaaga
    24181 tcgagggtgg gcgggcgacc tccgtgcgat ttcgcaacgt gccttccttc gtgcattcgc
    24241 gcggtatctc cgttcggacc cgttccgccg gcgaggttcc cctagacgtc tcttttgggg
    24301 gagccttcta cgctagcgtg gaggtgagtg ccctggggct cgcggtcgag cccgccgact
    24361 tgccacgctt ggtcgaggta ggacgcgaga taaaggacga gctgaacgcc accatggaga
    24421 tcgtccaccc cttggagccc aggctgcggg atgtctacgg caccatcttc ttcgaggacg
    24481 tcgaaggccc tggcttcgcc cagcgaaacg taaccgtctt cgccgacggc gaggtagacc
    24541 gctcgccgtg cggcagcggg acctcggcgc gcctgaccct cctccacgcg ggcggggaac
    24601 tcgcgctcgg ggcgacctta ggccatttcg gcgtcgtcgg aacgcgtttc gaggggcggg
    24661 tggtcgagga gacgaaggtg ggcgacttcg ccgcggtcgt gacggaggtc gagggcagcg
    24721 cccacctcac gggacaccac cagttcgtcc tcgggagcga cgaccccctc ggcccgggct
    24781 tcttgctgcg gtgagcggcc caagtgttgt tcgtggcatc aactcgataa agcggccgcg
    24841 ttcccccgtc gcggttctga tcaccgtgtt cacgcttgca gcgtgcggga acggcggcca
    24901 ggaccctgcc gcgcccgcca cggagccgag gcgtgaagat ccgtcggtag aggagttcct
    24961 cgacgacacc ctgcccgacg gggccagcgg caccgtagtc gcagcccgcg acggtgagat
    25021 cgcgcactgc aggggcttcg gcctcgccga tcgagaggcc aaggtcgccg cagggtgcga
    25081 caccgcctac gacatcatgt cgatgactaa gcagttcacc gccgcggcga tcctgaagct
    25141 ggagatgatg gggaagctca gcgtgagcga tccgatgagc gagttcgtcg gcccggtacc
    25201 cgacgacaag cgcgccatca ccgtgcatca tctgctgacc catacgtccg ggctgaccga
    25261 ccagctaggc ggcgactacg aggcgctctc tagggaggag atgctcgacg gcgcgctgga
    25321 gtccgagctg cggtcggccc cgggaaccga atactcctac tccaacctcg gatacagcat
    25381 cctcgccgcg atcgtcgaga aggcctccgg gacgagctac gagaggttcc tggcaagcca
    25441 cctgttcgag ccggcgggca tgaggcagac tggctacgtg ctccccgcgt ggaaacccga
    25501 tcaggtcgcg gtggagtacg acgagaacgg ggagcccaag ggcaagccgt tcgatcatcc
    25561 ctgggccgag gacgggccgt actggaacct tcgcggcaac ggcgggctgc tctccaccgc
    25621 gcgcgatatg ttccgctggc acgcggcgct cgaaggtgac caggtcctga gcgagagtgc
    25681 caaggacaag atgttcgagt cccacgtgcc cgaggaagaa ggcggcgaca gctactacgg
    25741 ttacggttgg gttgtctcgc ccaccgacga gggacggatc gtctggcacg acggagggaa
    25801 tggctggtcc ttcggcgtga tggcaaggtt cgcagaccaa gatacggtgg tgttctgggt
    25861 cagcaaccac gcgtacaacg atggggagtg gaacctcgag aatctggcac aaagactgac
    25921 actggggatc gccgaacgcg tgctcgacga cgggtgacgc atcagtgcgg acgtttcagc
    25981 cggggttgct atcgttccat ctcctgtagc gctagctcga acgctccagg ccccacccgc
    26041 acgtcgccgt cgtaggcgtg gtcgagtacc cctatcgtga acaggaggag cacgacgacg
    26101 accgtcaacg ctccggtcgc caccgcgtgc agtttgaaac tgcgcatcgc aaataggtaa
    26161 gtgaaccata ccgtgatcac cgctccggag actatgacga accacacaac cgagggtatg
    26221 ccctcgcgaa cctcggtcgt gcgcaggccc tgttctccct caggacgtcc atacgtccta
    26281 gcgcgtcggc gtagagttcg ctttggactg tggtctttgg gctgagctcg agcaccgccc
    26341 ttgccagttt gtgggcgcgc tgctcggcgc ggggactgac caggcctttg cccatcaggg
    26401 gccactcgtc ctgcacctcc ccccgggcgt aggagacagc gagcccttgg acgtcgctcc
    26461 tttcggggtc gggaaagttt tcggcctgcc agtagattca ttgtacggcg gcggcctccg
    26521 cttcgaccgt cagcctcgcc acctcgtatt gttgccacac taggaacagg gcaaacccaa
    26581 gggatatgcc gtagatcacg tacaacgccg cgtagatggc accggtggcg ccgtaatggg
    26641 cctgtaggag cgacaccggc acgaagcggt acaccagggc tagcccccaa cggaaagcgt
    26701 cagggccgcc aagattatga gcgcgccgga tagtatcgtg agcaaaaccc ctctcctcct
    26761 aagcacagca ccggctggta tgccctagca cccatcgact tgggtcgtct acccggtaca
    26821 gcaccaagta gctaactacg tccttgtgct gccatggtgc cacctgatca agcccagtgc
    26881 attcgtgaag gcttattcac cggattgcct agaagggatt ctccaaagtc cggattcagg
    26941 atcgtgaata tcctcccgta tcagaccggt gctgaacggc attgagccag tctcatggca
    27001 aagcggccaa cactacatct tgtgggacgg gatgattatg cagcttcacg cgtagaatag
    27061 aacagccaat tcctatagac ttcgaccaca gcgatttgat acgatcccgc ctgcaacggg
    27121 gacgtaggtt gcggctagag ggaaggttgg tggctttgta gccgattcga agttgggggt
    27181 gtgcgagttc tttgctacag cgcatgtgag atactgaaag gggagtgagt gtgtggaggt
    27241 ggcctggggt agtaactaaa gtcgtctcgt aaggggaccg ggagcgatcc caatctagag
    27301 gttgtttgta gaatgccggt cccgcacaga gatcttgatc atggaaattc gaggttttcc
    27361 agaaggaggt gaagccatga aaagagccct atatctcgca acaatgctgg ctcttctcac
    27421 gctgcttgtg gccgccccgg caatggcaca gggagccatt acagaccaat acgcagcagc
    27481 agcccagtac cagtacggag ccgcacaagc ccagtatggc gcggcggcac tccctccgac
    27541 gggcggatta gacctcctgc cgctagccgc aggactcgtg ctcctcttcg gcgggctagc
    27601 acttcggtgg cggctgcaac gtagataacc gacggccggg ggcgtgccta aacacctcac
    27661 tccgctgcgc ctccggcacc taagatgccc catcacccca gcccgctcct tcccgtgtcc
    27721 tcctgggcaa ctgctgcccg atacgaggcg gagagcggac gacgctgtga ggggccacga
    27781 aatagaagca cagcgcgaag ggcggagtag tcgcgttggc cacagagcgg tcgccacgtc
    27841 gacctcgaaa agacagtgcc ttctcgtaga agtcgcatag accgagatgt gtcattcggc
    27901 ctatgggcca ccctgcccct agggtacact gtccttgtta catgacgaag agggggagat
    27961 ccacatggct agacggacgc tctatctacg gacgctgcta gggaccctga cggccgcggt
    28021 agtggtggct tgtgcggtgg cgttgttggt ggtctcagag gaggcggaag ctacttttcc
    28081 tggcaagaac ggcaggatag ccttttcgtc ctacgatgga aacgactacg agatctacac
    28141 cgtcaactcc cgcggcgggg ataagttcca ggtcaccgac aacgctaggg acgattacag
    28201 tccttcctac tcgcccaacg gcaagaggat cgtctacgtg gagctagaca gaaacgacgc
    28261 cgagctctac acgatcaatg tcggggggag gggtaagacc caactcaccc acaacaacgc
    28321 ggacgaagcc gctccttcct actcgccaga cggcaagaag atcgtctaca tggtgtatag
    28381 caacgcctcc ggggtcacgg agatctacac gatctacatc ggcgggggtg gtaagaccaa
    28441 actgacgacc ggcgggcagc cttcctactc gcccgacggc aaaaggatcg tctattggga
    28501 ctaccacaaa actgcaaaaa atccccaccc ggacacggag atctacacga tcaacgccag
    28561 cggaggggat gagacccaac tcacccacaa caactcagac gagttcgccc ccgacttttc
    28621 gcccgatggc aaacgcatcg tcttcgcggg cctggatggc ttggagcgta acaatgccga
    28681 aagcgacatc tacaagatga aagtcggcgg gggcgcaaag accagactca ccaacaacaa
    28741 cacatacgac acttatcctt cctactcgcc agacggcaag aagatcgcct tcgcggtgtc
    28801 caaaggcagc gccgggggcg acatctacac gatcaacgcc ttcggagggg gtaagaccca
    28861 actcaccaac gacaactcag acgaagccgc tccttcctgg gggagccgcc agtagcgact
    28921 tcacctgcag gcgaagtttg tgacgaagtg tgcaccccaa aacctccttg acaaaccgcg
    28981 cgagcctatt cataacaaac ggtactgttt agtttatctc aataggataa tggacgagct
    29041 cggtcaagag gaaggcatgc tcgacctcga acacacgaaa gtctcgagga tcgaagccct
    29101 gggacttcct ggactacgtg acaagggaag cgctacccga gtttccgctc atcgctttcg
    29161 gtatctggcc cgagcatgta aagcacaggg agccggcggc gagagggaag tcagtgggat
    29221 aaatctggtg acctgtcgta ggtgcctttt gtaaaggcca tacctgatgc tatattaagc
    29281 gtatgcgcgc cagggatcac aaggttcaga agtcggactc ccgactatag aatcctgcgc
    29341 ggattccccg atctagaacc accccacgcc cttaaccgaa ggagtctctc gtgagcgaga
    29401 cgaaacagag aaggacttac gatcccacgg acctcgaacg ccgctggcag gagcggtggg
    29461 ccgagagcgg cctgtacaag accgacgaag acgagagcaa gcccaagcat tatgcgctca
    29521 cgatgctccc ttacccgagc ggggatctgc acgtggggca ctggtacccg atgacgccct
    29581 cggacaccag ggcgcgcttt atgcggatga acggctacag ggtgttcttc cctataggct
    29641 tcgacgcctt cgggctgccg gcggagaacg ccgcgataag caggggcata caaccccgcg
    29701 aatggactta cgcgaacatc gagcggatgc gcggccagct caggcagatg ggcgccatgt
    29761 tcgacttcga ctcccaggtc gtgacctgcg accctgaata ctaccgctgg aaccagtggt
    29821 tcttcctcaa gttcttcgag aagggtctcg cgtaccgcat ggaggcaccg gtcgactggt
    29881 gccccaggga caacaccacg ctggcgcggg agcaggtcgt ggggccggac aggcgctgcg
    29941 aacggtgcgg gacgccggtt atcaagaaga acctgccgca gtggctcttc aagatcacga
    30001 actacgccga ggagctgctc gacttctcga ggatagactg gcccgagcgg gtcgagacgc
    30061 tgcagcggaa ctggatcggg cgctccgagg gggccgcaat aagattcgag atcgaaggct
    30121 acggtccggt cgaggtcttc accacccgcc ccgacacgct cttcggcgcc acgttcttcg
    30181 tcatcagccc ggagcacccg gcggtcgggc agataacgac cccggagcgg gccgacgaag
    30241 tgcgcgccta cgtcgagagg gcagcccgga tgagcgagat cgaccgcacc gacgtgacga
    30301 gggaaaagtc gggtgtcttc accggcgcct acgccacaaa cccggccaac ggggaagaga
    30361 tccccgtcta cgtcgccgac tacgtcctga tgggctacgg tactggcgcg atcatggccg
    30421 tcccggcaca cgacgagcgg gacctcgcgt tcgcaaagaa gtacggcatc gaggtcagga
    30481 ccgttattgc tccgccggat tggaacggcg aagatctcgt agaggcttac gctggcgagg
    30541 gcacgatggt cgactcgggt aggttcgacg gcaccccgag caccgagggc aaggagaacg
    30601 tgacggcctg gctcgaggag cgcggcgtcg gcaaggccac ggtcacctac cgccttcacg
    30661 actggttgat cagccgccag cgctactggg ggaccccgat ccccataatc ttctgcgacg
    30721 agtgcggcat cgtccctgtc cccgaagaag acctccccgt cctcctcccc gatgaatccg
    30781 agttcatgcc cacgggcgaa tcgccattga agctcgaccc gaccttctac aataccgagt
    30841 gtcccggatg cggcggtcct gccacgcgcg agacggatac gatggacacc ttcatggact
    30901 cctcgtggta ctggtaccgt tacctctcgc cgcactacga cgaggggcct ttcgatcccg
    30961 agcggggaga gaagtggctc cccgtcgacc agtacaccgg cggcatcgag cacgccattt
    31021 tgcacctgct ctacgcccgc ttcttcacca aggtgatgcg ggatctcggt ctggtcgact
    31081 tcggcgagcc catgacccgc ctgcgcaacc aggggagcat cctcgggcca gacgggcaga
    31141 agatgagcaa gagccggggc aacgtggtca acccgcagga gtacgtagac cgcttcggct
    31201 ccgacgcgct ccggggcttc ctgatgttca tcggcccgtg gaatgaaggc ggtccctggg
    31261 acggcaaggg catagagggt gtctcacgct ggctcaggcg cgccctctcg ctcgtgacgg
    31321 gaggcgactc ctcgggcggt gaggcagacc cgacggagct ggagcgccgg acgaaccgcc
    31381 tgatcaagaa ggtcacagac gacctcgatg ccttccggtt caacacgtcc atcgccgcgc
    31441 tcatggagca caccaactac ctgctcgcgg taagaggccg cgtcgggaac gacgagtggc
    31501 acgaagcctt gcgggccttc gtcacggtac tctcaccgtt cgcgccgcac cacgccgagg
    31561 agctgtgggc ggagatgggg gaggagtact cggtgcacga gcaggcgtgg ccaggctatg
    31621 aggaagccct gatccaggcc ccggagataa cgctcgtagt ccaggtcaac ggcaagctgc
    31681 gcgaccgtat cgacgccccg gcagatatct cggaagaggc ggccagagaa ctggcattgt
    31741 cgagcgccag agtccgtcct cacctggaag gcaaagagct ccgcaagagc atctacgtac
    31801 ctggcaggct cgtgaacctc gtcgtaggat agcgatccac agtgacccta cgccgctgcg
    31861 agagcctcag aaggctgtgc gatgctgttg taagggtagg atgaagcggc agcctgtcca
    31921 atagtagcta gaaagaggcg ccaatttagc tctgagttca cgcgaaggag gttcctacag
    31981 gctgcggggg gagccgccgc ctgggtcgcg ctcggcggca ccctgggctg cggtccgatc
    32041 ccgcgcgccc gggcgaaggc cgcgtccgca cgcgcagggc aggcatggaa cttccgctcg
    32101 cgtcctgatc tccacccgcc ggtcatcgag gtggtcaaga aggcgcgcga tactgctcgc
    32161 ggctacatct tcgtcgcccc gaagaacgga ccagacgagg ccggtcctgg gcaggacgga
    32221 tgcatgattc tcgacaacga cggacagccc gtgtggatgc gcctcttgca gaacgaggat
    32281 atggatgtga tggccttcag agcgcagacc tacaaggggg agagggtgct cacctggtgg
    32341 gagggactcc ataccggcta cggccagggc cactacgtca tcctcgatag ctcctaccgc
    32401 caaatagccc gcttcggtgc ccaaaaccct taccagggcg accaccacga gttcctcata
    32461 accccacacg atacagccct tatcaccatc taccacaaag tagcgatgga tctctcaggg
    32521 gtgggaggtc ctgtggatgg ggaggtgcta gaggggatag tccaggagat agacatccaa
    32581 agcggggagg cgctctttga gtggcacagc ctccaacacg tggggctaga tgagtccctc
    32641 tacgcgcccc ctgccgacct tgaggcgcca tttgactact tccacatcaa ctccatagac
    32701 gtctatgacg aggatcacct gctcatctcg gctcgcagga cgagcgcggt ctacaaggtg
    32761 gacaaaagaa cgggggaagt ggtgtggcga ctgggtgggg agaagagcga cttcgagatg
    32821 gggcacggca ccaggaccga ctggcagcac gatgcccgcc accagggtga cggcaccatc
    32881 accatcttcg ataacggcgg ggtcgaaaaa gacgtacagt cccgtggcat agtggtcgag
    32941 atcgacgaag acgctatgag cgccacgctg gtaggcgagt acactcatcc caacaagata
    33001 ctcgcggcca cccagggtaa tctccaggtg ttgcccaacg ggaacgtgtt cgtcgggtgg
    33061 ggcagcgagc catcctactc ggagttcggc cctgacggca aactgctcta cgacgcgagc
    33121 ttcccctccc aggtcgagtc ttatagagcc ttccgcttcc cgtggaaggg ccaaccgaag
    33181 gatcggcccg cggtcgtggc cgaagccggg ccggacgacc gggtaacggt ctacgtcagt
    33241 tggaacggcg ctacggaggt aactgcctgg cagatactgg ccggtggaag ccccgacgag
    33301 ctcgagcccg caggctcggc ccgccgcagg ggcttcgaga ccgtcgtaac gctgcaaacc
    33361 ggcgagcctt acgtcgccgt cgaagccaaa gacggctccg gcagggtgct cggtacctcc
    33421 gaggcggtcg agccggtagg gcactccgct tcgacgggcc aaacccgcac gtaaggaacc
    33481 ctgcagggct ggatagtgga caaacgccgg atacagcttc gatggttcga ggccggcgcc
    33541 gcgctcgcgg tgatcgcggc attcgccgtt atcggtccgt tgaggggcac ctttgcgccg
    33601 ataccctggg tcatgttgct ctgtaccctt atcttgttct tgacgccggg cgcactactg
    33661 gtacgctggt tctggaggga ctactttttc ggcgccgcgc tcgcgccggc ggctttcgtc
    33721 acgagcgtcg ggctcttcgc cctcctggcg gtgcccatgc tcatcttgca gggtaccctg
    33781 gacgcctacc tgtgggcctg cggcacgatc gtcgccgcgt gttggctcgc cgccgtggtc
    33841 ctggcgttcc gccccgagca ggcggaagaa gctcagagcg gcttctcgct ctccgaccga
    33901 ggggggatgc tctggatgcc gttcgccgcc ctcgtcgctg cgctctcgta catcgccagg
    33961 aacacggccc cgagctacta cggtgacatc tggatctatc tctcgtgggt cagggagtac
    34021 ctcggcggtg gcaggctcgc ctccgtcgaa cccttcttcg gtagtgaggt gggcctctcg
    34081 cgcgccagga tcaacggctg gatactggag caggtcgcgg tctccagagt ctctggggtc
    34141 gacccgatag aactggcctt ttcatacctg aaccctgcac tggtggttgt ggctttcctg
    34201 gtcttctacg ccctggcgcg catcctgttc gagagcgaga aggcggcgct gttctccgga
    34261 tgcctgtacg ccctgttctt ctttatacac ctcagccagt cgcgcattgc ctggggaggg
    34321 gagttcgtcc agcgcctgcc cgaggacaaa ctggtagcca agttcctctt tttgcccctg
    34381 gccctcgcgt tcgcctacgc ctttatgcaa ggcggggcga agaggtactt catcggtttc
    34441 tcgttcctgt gctgtgcggt catggccgtg catcccatag gcctggcgat catcggtatc
    34501 tccatggccg ggttctcggt tttgcacctc gccgccgacc cccgcaggcg ggcggcatgg
    34561 atcaggatct cggccatggg cctgaccggt gtcgccataa tcgccgttcc ggcggtcctc
    34621 gtctcgtcgt ttaccgatga gtcgctgtcg aatgccctgg ccgactcgga catcaactcc
    34681 ggcgaccccg acgtgctacg gaacatgatc ttcgtcagcc cggagaggaa ccgcatcttc
    34741 gagctggccg acggctccta catcatgcac ccttcgttgc tcctcgatcc cgtgatagcc
    34801 gccgcattct tgatcggact ccctttcttg ctctggcgct tgaagcggag tctggccgca
    34861 caactgctct tcgggacgat gtacgtgacg acgatcatcg tctacgtacc cccgatcgcc
    34921 acgttcctcg gcgataacgt cgtgctcccc ggccagatct ggcgtctggc ctggccggta
    34981 cagctcgccg ccgtactgac gcttggctgg ctcgtgtggg gcgccacaga cttagtggcc
    35041 gcgtggctgc gaagcttccg gccggcgcgt ctgctggcgg gggcgctacc tatcctgctg
    35101 gtcatcgccc tggccgcagc ggccgtgccc caggcgcgtc agggtatgga gtccattcag
    35161 gcgcacaggg aagcgagccg cgagtctggc ttctatcctt ccgatcccat ctttccctgg
    35221 ttccgcgagg agattcgctc gcccgtcgtc gtgctcgcgc ccgacatcca gagcgcccgt
    35281 atccccgctt actcctcaga ggcgaacgtc gtcagccgca ggggaggatt ggtgttgagg
    35341 gtcctccccg agcttaagga gcgggcaccg ggccagatcg aagtgcccca ggggtccctg
    35401 gacgtgcagg agttcttcag tggggccacg ctgcagcagg acatcgagat cctgcgccgc
    35461 aacgacgtgg actacgtgat ggtggccagg gactcgcaac tggccgtatc gctcgaacgc
    35521 ctctccggct tcacccccgt agagacaccc agtgagaggt acgatctcta cgccgtggac
    35581 ctccaggagg tggcctcgga atcaggggct ctgccgagtt cgcccggggc ccattctgta
    35641 gaatggccct ctccggatac tcgacctggg aggcttatct cttgacgttt ttcggaaggc
    35701 tcggcgatag ggtggtcggc gccccttcga tactcgcggc cgatttctcg gaactggccg
    35761 aggaagtgcg gagggccgaa aggggtggag ccgcgctcgt acacttcgat gtgatggaca
    35821 accacttcgt ccccaacctt accgtgggcc ccgccgtcgc ggcctcgctc gccgccgcgg
    35881 taaacctccc tattgacgcc cacctccagg taacgaaccc tgacaacctg atcccatctt
    35941 tcatcgaggc cggggtcgtc agcatctcgg tccaacccga ggtggtcacg cacctgcacc
    36001 gcacgctcga cctgatccga accggcggtc gcgaggccgg cgtcgccctg aacccgacga
    36061 ctcctcccga gtccatagag tgggcgctgc cgtacgtgag ctacgtaaac gtaatgagcg
    36121 tcaaccccgg cttcgggggt caggccttta tccccgagat ggtcgagaag gtcaggcgcc
    36181 tcaaggagat gacggacgcc cccgtggaag tggacggcgg cattacggtc gaaaccgcgc
    36241 cgctcatggt cagggcaggc gcccaggtcc tcgtcgcagg ttcggcggtc ttcaagggcg
    36301 atccggcgac cgagatgcgc aaaatcatcg aagccgggca gggagctagt cagcatctcg
    36361 gctagtcagc acgctccttt cagccggtgc gtgcgcaaac gcgaagcgtt tccttgctgc
    36421 cctgctcctc gtgtggtata ggttgctcag taactttcgg gggcgggtgg aattcccgca
    36481 ccggcggtga gcctgggtag ttcgcgaacc gcccctaccc ggggagcccg cgaacctctt
    36541 tggaggttga gccggtgaat gctcgtattc ggtgcgagcc gtagtccggc gccgacggtc
    36601 agagtccgga tgggagaaag gaagacgtgc agcaggattc gatgatggat cttgcccgtt
    36661 gcctcgccga gcgcgggcgc tactcggttg caccgaaccc cctggtgggg gccgtcgtcg
    36721 cccgtgacgg aagcgtgcta agcgagggct ggcattccag ggccggggag gaccacgccg
    36781 agatagtggc cttgaacggg gccggaggaa cggcgcgcgg gcacaccttg tacgtcacgc
    36841 tcgagccttg caaccattac ggtaggacgc cgccgtgcgt cgaggccatc ttgcgatcgg
    36901 gtatctctcg cgtggtggtg ggacacctgg accccaaccc gaaaatgcgg gggcgaagcg
    36961 tggggatctt gcgcgacgct ggggtcgaag tcgaggtagc cgccgatccc gtcttcgagt
    37021 ggcagaatga gcagtacttt caccacatgc gtaccggaaa gccgttcgtc cacatcaaac
    37081 tcgctgcgac gctcgacgga aggatcgcgg cggcgggggg cgacagcaag tgggtgacgg
    37141 gcgaagccgc gagaaggcgg gcccacgagc tgagggccga ggccggggcg gtactcgtcg
    37201 gcgcgaacac cgtccggacg gacgacccta tgcttacggc aaggggcctt cccgtacctt
    37261 cgccccttat aaccagggtg gttctggacc cccacctgac cataagcccg gagagcgagc
    37321 ttgcgaggag cgccggagaa gcgtccgtcc tcgtcttcgc ctcccaggcc gcgctcgatg
    37381 gccgcgaaac caagctggaa agggtcggcg tcgaggttgc cgccgccccg ccctctgagg
    37441 agggcctcga tcttctccat gtcttgggtg agctgggtga gcgtgggatc aagggtctgc
    37501 tcgtcgaggg cgggggcgag acggccacgc gctttgtccg cagggggctc gccgacaagc
    37561 tcaccctgtt ctacgcgccg aaactactcg gctctgaggg cgtgcctatg atcggtgcgc
    37621 tcagggctac gaaggtggca gagtcgcttc gattctcggt ctcggaagtc gaaaaagtag
    37681 gcgatgacat cgccgtgacg ctctatcccg tcggggagga ggaacgtgtt caccgggctt
    37741 gttgaggaag tagggcgcgt ctcctcgatc ggggagggcg agatgttgcg attgagcatc
    37801 tccgccggca gggcgtctga gggtgccagc gctggcgaca gcgtgtgtgt gaacggggcg
    37861 tgcctgacga tcggcgaggc cgatggtgag acgctcacct tcttcgccat gcccgagacg
    37921 ttgaggcgca cggcgctggg gggcctcgtc gtcgggagcc ttgtcaacct ggagcgggcg
    37981 atgtccgcgg ggagcaggtt cgggggtcac atcgtgcagg gtcacgtcga cggcgtaggt
    38041 gaggtgctcg gggtgaggcc ggaaggcgac gcggagatct gggagttcgc ggcccccaaa
    38101 gccgtgctgc gctactgcgt cgagaagggc agtatctgcg tcgatggtat aagcctgacg
    38161 atagtctcta tcggcgcgaa cgcattcacc gtctccatcc tgccccagac ccgcgcgaac
    38221 actaatttgc gggaactcgg gacggggggc agggtaaacc tcgaggccga cgttattggc
    38281 aagtacgtag agcggctgct cgggccgcgc ttggcaggaa gactttcaac tagcgagagg
    38341 agtgtcgcag atgcctttta gtcccataga ggagatcgtc gaggatatca agcacggcaa
    38401 gatggttatc gtttgtgacg acgaggaccg cgagaacgag ggcgacctga caatggccgc
    38461 cgaactcgta acagccgatg acatcaactt catggcctcc cacggtcggg gtctgatctg
    38521 cctgccgatg gcgggggaga tcgtggaccg cctcgacata cctcagatgg tcacccacaa
    38581 cgcttcgagg atgggcacgg cgttcacggc ctccatcgag gcgaaggagg gcatcacgac
    38641 gggcatatcc gccgccgaca gggcccatac ctgcagggtc gccgtggatg aggcgacagg
    38701 cccggaagac ctcgtgatgc caggacacgt cttccctttg cgggcgaagg acgggggcgt
    38761 cctgcagcgc gccggacaga ccgaggccgc cgtcgatctc gccaggttgg ccgggttcaa
    38821 gcccgcgggt gtgatctgcg agatcatgaa agaggatggc acgatggccc gcgtacccga
    38881 cctggagagg ttctccaaag aacacggcgt caagctggtg acggtggccc agatcatcga
    38941 gtaccgtcac gccttcgaga cgcaggtcag gtgcgccgtc gagacgcggc tacccacccc
    39001 gttcggtgag ttcaggctgc gcgcctacga gaacgacata gacgaactta cccacgtggc
    39061 gctcgtcatg ggcgagcccg aaggtaagga cgatgtgctc gtcagggtcc actcggcgtg
    39121 cctgacgggc gacgctctgc actccttgcg ctgcgactgc ggcgagcaac tggaggccgc
    39181 catgggacgc atcgcggagg aaggcgaagg cgtgatcgcc tacatgcagc aggaaggtcg
    39241 gggtatcggg ctcctcaaca agatgagggc ctaccacctc caggatgagg ggatggacac
    39301 tgtcgaggcc aaccagaagc tcggtctcgc ccccgacctc agggactacg gggtcggggc
    39361 ccagatcctc aaagacctcg acctgaagcg catacgtctc ctcaccaaca atctgacgaa
    39421 ggtcgtgggc ttgaggggct tcgggctgga gataaccgag cgcgtcccca tcgagatgga
    39481 gcccaacggc cacaacgagc gctacctgaa gacgaagcgg gagaagctga accacgtatt
    39541 cgagaagttc taggagggga gcgtggaggg agccaaccac atcgagggga atctctccgc
    39601 tgaggggagt tccttcggga tagtcgcctc gcgcttcaac gattttgtcg taaaggcgtt
    39661 gctggaaggg gctatcgagg cgatccggcg tcacgggggc gacgcggagg ccgtcgacgt
    39721 cgtttgggtg cccggttcct acgagatacc cctcgtcgcc agagagctgg ccctatcggg
    39781 acgatacgat gctgtgatct gcctgggggc cgtgatccgg ggcgcaacgg cccacttcga
    39841 ctacgtcgcc ggcggggccg ccggaggcat ctccaacgtg gcgctggaga gtggggtgcc
    39901 cgtgatcttc ggtgtcataa ccacagagtc gatcgaacag gctatagaac gcgccggcac
    39961 gaagatgggt aacaagggct tcgaggcggc ggtctctgcg atggagatgg cagacctcat
    40021 ggcgaagctt cgcgaacgcg cgtcgcaaag ggacacgccc ctcgagaacc cctgagggct
    40081 a
//
LOCUS       DATMVF010000270        25064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_585744, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000270 DATMVF010000000
VERSION     DATMVF010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 25064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25064
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585744"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VN178_13995"
     CDS             <1..463
                     /locus_tag="VN178_13995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS82126.1"
                     /translation="SYYERVLDVSPALGNTALTLMLFGGAAGTLIMGPLADRFGRRTV
                     LLASMLVLPPLIFCFTLAGPTFGMALLILVGAATVGTFGVTVVMGQEYLPGRIGLAAG
                     ITMGLSIGLGGVGAPLLGLIADNAGLSFTMLVIAVLPVPGLVLALTLPRGR"
     gene            573..3164
                     /locus_tag="VN178_14000"
     CDS             573..3164
                     /locus_tag="VN178_14000"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWS82127.1"
                     /translation="MNLFHSIGVFAYRFRWAILLVWGMVLISSAFFAPGLSGQLKGGG
                     FDGANSEAERVQDLMSDEFGLSPATLTVVFTGDGLPARSEEFQDAQESALAEVRKLDD
                     VRQVISYADAEDPRFISEDGMKSYAVVTSNVSVDATRNVVDEVRSKVRSGELETYVTG
                     APAVYQDLEEASNEDVKEAEKYAFPFAVIILIFAFGTLVAAGVPVLIGGASVVTALAT
                     LYFIAGVYDMSVFTLTLSTMLGLGLGIDYALFFVSRFREELEDYPTVEAVPRTVATAG
                     RSIFFSGTAVLIGLSGLLFFPFMFMRSIGVAGVVVVFVSVLAALTLLPALLGVLGPRI
                     NRLAIRRPRDGAGSAFWSRSAEVVMRRPLAVILLAAAILGTLLYPVGHMKVGIPEATV
                     LPQEYESRAGDDILKSNFEYAALNPMEIVATLEDDPLSARGLSDTRELGENISGADGV
                     SRVESVYTLAAAAAEDYAGRLADAREQARKKAASRVDAIVQRQIEEQTQNETDRAVNE
                     QLSEIEADYGAVPPGTEERLRTDIRPRVESEVRASEGRIRDEVERRLAEEVDRKVPDL
                     PEGISADGTVTPEGVAIFLNLPAARRSEDLQDTLDTYVAGDRTLLRGVTEANPYTQGA
                     YGAVDAVRAVEAPDGTSFLVGGLSAGQKDFISSLYGKAPLAAAFVIGVTYLILLLTFR
                     SVLLPLKAVLVNILSLTASFGALVFVFQDGNLSGLLDFTPLGFVDATLPILMFCTIFG
                     VSMDYEVFLLSRIREAYENGDSNAASVSKGLVATAGIITSAAAIIIVVTGAFALTGIV
                     LTKAIGLGLAVAVFVDATIVRILLVPATMRILGDWNWWPGGRKVTFGADPEEIAKDWP
                     PDRESPR"
     gene            3210..3620
                     /locus_tag="VN178_14005"
     CDS             3210..3620
                     /locus_tag="VN178_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWS82128.1"
                     /translation="MLEFERILKDRGYRLTNQRRIIVHELDGERHLSAEEIYDRVKGE
                     HPELGLSTVYRTLDLLVELGIVRKDDFGEGYSRYELATERMHHHARCEQCGNVIEFNE
                     ELMEYLALQVERETGFVTDWHEITLYGRCADCSE"
     gene            complement(3852..4610)
                     /locus_tag="VN178_14010"
     CDS             complement(3852..4610)
                     /locus_tag="VN178_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HWS82129.1"
                     /translation="MKITVVGSGTVVPRLERRQSCVVVRAGGQTLVFDLGSGAVRGML
                     ESGLDPFAVDRIFFTHFHPDHTVDVVPLLFAINYGAPEKRTLPLYLSGPEPFLRFWGS
                     VMEVWGKWMSGDHATLLSELSQECPSPIDLAGCRLSWAPAEHRRESIAYRLDAEGGAF
                     VYTGDTEYSGSVVELARGADTLLIECSFPDETPVPGHLTPSGVARIASEAGVRRVVLT
                     HIYPAADDLDLVTEVGRGYEGEILVAEDALELEV"
     gene            complement(4752..5384)
                     /locus_tag="VN178_14015"
     CDS             complement(4752..5384)
                     /locus_tag="VN178_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWS82130.1"
                     /translation="MKGDKRMYLLLSTAAFLACSVVAGAGLFQPLDVLILDAAQRTDT
                     GVLDAIGLVTSVAGGVEFVAVATVALATSLLLLGRGPLALRLVIAFLVTGLIELAAKT
                     MVPQPPVPEDAMRTPDPSLLDVSTPYPYPSGHMLRSVLLLGAIYLLWPSRPARIALLI
                     FLAASAVSRVYLGTHWPSDVLGGALLGLAGLAWTFEKQSAFSGQPSAKDP"
     gene            complement(5548..6432)
                     /locus_tag="VN178_14020"
     CDS             complement(5548..6432)
                     /locus_tag="VN178_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HWS82131.1"
                     /translation="MKASFEKRKENHKIERESETPELLAKYLAHIGQGNLLTHAEEID
                     LSVRAKAGDKRARQRLIEKNLRLVVSVAKKYRGYGLPFEDLIQEGNIGLMKAVEKFDP
                     DRGFRFSTYATWWIRQAVQRAVADKGRTIRVPVHMTEKIRKVSRAMSELALEIEREPT
                     EEEVARRLDWDPDEVRLTMRAMPDATSLDQPVSSEETASQLGDFIEDDKVSDTPDTVM
                     REMETEHLKEAIEGLPERARYVLVRRYGLDDREPATLAELGDELDISRERVRQLQREA
                     ERILKGGEYGRVLRDAVA"
     gene            6606..7592
                     /locus_tag="VN178_14025"
     CDS             6606..7592
                     /locus_tag="VN178_14025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWS82132.1"
                     /translation="MARFVVALRCLAAGAVILGLMENASRIPDRISEDIANIVACWQA
                     RRRGDNEDGVYDRMLAGMEELVSVFVAFLRSTETVETFSRGGATRAMVRRIAEYQHDL
                     GRDAVGVIEDFSVLRRCIWRSVEAGVDLGGLHGGEVSGFFAKLMQASDWLTEAGLEAF
                     DAIVRRDMEQALGRATATDLVTGLPDRDQLNRLLLPRAIDSYERFSVAVFDVAGFTET
                     VAAGKIKRARKVLRTLAEAVQASSPQGSLFARFGDDEVCVILPGESGEGGYRLAERVL
                     GRLVEGMAEFEVDVGVAEYPAHGTDPGELMGETLKALKMAQRVGGGGIVVAR"
     gene            7610..7930
                     /locus_tag="VN178_14030"
     CDS             7610..7930
                     /locus_tag="VN178_14030"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWS82133.1"
                     /translation="MIAIFNSLPVKEGAADEIVNRFAESRGYVQGFPGFVSMEVLESD
                     AGDEVLVVTRWRDRESFEAWIDSDAFSRAHGRSGAGELLTGHPRMTSYRVAVERAPEA
                     SGPG"
     gene            complement(7934..8149)
                     /locus_tag="VN178_14035"
     CDS             complement(7934..8149)
                     /locus_tag="VN178_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82134.1"
                     /translation="MREIRRTCLVDWVRMALRFVLERGDKVRERLAFGILILLLICLF
                     AFTFAVLGKGEYEPTGEKPQLLSQSLA"
     gene            8256..8951
                     /locus_tag="VN178_14040"
     CDS             8256..8951
                     /locus_tag="VN178_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWS82135.1"
                     /translation="MAVDISLTEYTKLQEQECRSLLRLLDEMGIATADRVYGPGDAIY
                     NEGEYGDALYVLTSGVLKLFRPYSGSKEATLRLLKPWDIFGHLAFAGETRQRAYAEAV
                     TECRVTKVPKIFVERAVRQEPQAAFKLMTLLELRLVQYEELVQCLLPRETEVRLANLL
                     PLLAQKFGQSRDGLVTIGLRLTHQDLAAMVASTRESVTKVLNEMRNRDVIEVEAGRIT
                     LKDPAALAVLSGI"
     gene            9064..9894
                     /gene="uppP"
                     /locus_tag="VN178_14045"
     CDS             9064..9894
                     /gene="uppP"
                     /locus_tag="VN178_14045"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HWS82136.1"
                     /translation="MLELVQAILLGVVQGLTEFLPVSSSGHLLLGQYFLGMDQDRFGL
                     TFDASIHTGTVLAVVVYFWRDLMRMAAAFVASLRGPDLADRDQRMVYLILVACIPAGV
                     VGLLFQNFFEEEVRSPWLVAFNLVLVGALFIVGEVVGRQTRTSDKLGFKEALGIGLAQ
                     TAALFPGVSRSGATITIGLFLGLRRDEAARFSFLMSVPITTAAALLSLVDAFGSGIDS
                     NDVLLFLVGSITSGVVGYLAIRFLLNYLSGHSLLVFAYYRFALAAVVVVLLLFGLGGF
                     "
     gene            10046..11176
                     /gene="pheA"
                     /locus_tag="VN178_14050"
     CDS             10046..11176
                     /gene="pheA"
                     /locus_tag="VN178_14050"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWS82137.1"
                     /translation="MFPPDENTTHGPQAPDSPDEMHTLRESIDSVDEEIVRLLDRRAW
                     LARRIGEIKHKNGLAAYAPARERVVLDRVATLSDGEFPRRGLQAVFREIISSSISLEA
                     SLKVAYLGPEATFTHEAALRSFGTSIELEPQTTVAEVFARVERGEVEHGVVPVENSME
                     GAVTHTLDELMNSPLKICGEVYLPVSQNLISAEQSIERVSLVCSHPMALAQAASWLRR
                     ELPAARLDEVDSTAEAARRASLEPGVAAIGSVLAAGAHGLTVLARNIQDARANTTRFI
                     VLGRAWAARTGKDKTSVVFSVKDRPGVLKDALSAFAEEGINLTRIESRPSRKRAWTYV
                     FFADFLGHPEEDRVKRALEALEEHCPYVSLIGAYPEVSPEVV"
     gene            11191..11448
                     /locus_tag="VN178_14055"
     CDS             11191..11448
                     /locus_tag="VN178_14055"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWS82138.1"
                     /translation="MDFKAAAREVLEEVGHPLHYTDITEIALQSGYLQSSGRTPQNTM
                     RARLSVDVRDNPNTYFAQVAPGVYGLKSNPGGGLDSSVGRE"
     gene            11507..12286
                     /locus_tag="VN178_14060"
     CDS             11507..12286
                     /locus_tag="VN178_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82139.1"
                     /translation="MVELADKVSEAAWRIKNLKDYDERLERAARRFDEISRERPEPAL
                     VIYASALKKSGRSEDEIRGMVREFHETFEDEEEPLTITHASGIMSVEGDDWFFGDADL
                     RVLTGRLLGQFQHRVAQYNYVDESEVLRRWADGYDTRLFVRRRIHETEPVVGVVGDLD
                     LTLFQYLRVAAGGNTLVPTENVSRALGALGYEGDEYETLAQAEGLALYLDLPAPIVGE
                     MLEDLGREGFTDFPQPPSGSGENDGEGVAPEGDSEENEEKA"
     gene            12415..13044
                     /locus_tag="VN178_14065"
     CDS             12415..13044
                     /locus_tag="VN178_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWS82140.1"
                     /translation="MADSRKRIALKPHMDRIREWVETGKTDDWIADALGTSASSVQSF
                     RSRNDIYRRERGPREPESVFEGVLDHGERDGWGLWLDPSIAEDPIWKEHWAGVEAVVV
                     KVAEDSIVLEADGGESATADDLAMPVTIPDGTSDEQGSGLPRERGRVKWFDAEKGYGF
                     LVRPTGDDLFVHHSEVQGDPSELSPSGEVEYEVGRNDRGPNARSVRPLG"
     gene            complement(13073..13708)
                     /locus_tag="VN178_14070"
     CDS             complement(13073..13708)
                     /locus_tag="VN178_14070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWS82141.1"
                     /translation="MLLIRHGQSTANARGIWQGQMEFPLSERGRVQAGLAGLGLAGEP
                     FDGLYSSPLSRAFQTAEIIRDRTRFAGEVVPVDGLSERSGGILEGHTWAEQEQRNPEL
                     AKKFLAIPEEERWALVGAETDEEIIARFEEALSSIRALHPRGSRIVVVSHGGVMRAYL
                     RELFGPEVMPGAQRAANASITRLQWGEMGPRLLEVASTAHLSEDPGPSTTE"
     gene            13817..14662
                     /locus_tag="VN178_14075"
     CDS             13817..14662
                     /locus_tag="VN178_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82142.1"
                     /translation="MIRRDGEQTPEEGVEAVQEENTDPIQIERDAPRPVTILRVAGEL
                     EERGGTILELFKEIESPLGRVILPIYLRQDDRDFFVEVATGPWDDRKSGEALDKAAIL
                     RSSEHAGAGLEILSGYPVPPEVEFYFGTSPAALLQLDLARLTPERPEVCAGLFREVGS
                     NHWGVDLDYEPGYLPLIEELLMAALEGDEAHNSTPLSGGLVSGLGCFLGEIIRRNVSP
                     PGVWRPPEEWGEGPVVEIGDFVLDPVGKARAFLEYGPEESLTLYAEYVLERRDGGELP
                     PDLQA"
     gene            complement(14653..14901)
                     /locus_tag="VN178_14080"
     CDS             complement(14653..14901)
                     /locus_tag="VN178_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWS82143.1"
                     /translation="MDATLQKTGIRLYTGDYCPFCRRVRNELERLGLDYESVDADADM
                     REEVIRLSGQRAIPILTIGDEVLVDSSHIIRELRNRYA"
     gene            complement(14983..15477)
                     /locus_tag="VN178_14085"
     CDS             complement(14983..15477)
                     /locus_tag="VN178_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWS82144.1"
                     /translation="MDRDLRDRDSILAGLRHLTHGVYVMGTRRGRQSNAMTASWVMQT
                     SERPPCLAVAVRSDRYTHDIVLESETFALSVLRDDQVDVATHFADTSGEYHDKLQGIP
                     YGLTPGGSPFLLDCLAYLDCKVIDRARAGDHTVVVGEVTASDTLDESYPLIYDPAEYE
                     EALR"
     gene            15561..16550
                     /locus_tag="VN178_14090"
     CDS             15561..16550
                     /locus_tag="VN178_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HWS82145.1"
                     /translation="MGLSGTLIWLGILQAVIYLLFIRAIDLYEREPLRYVIPVFIWGF
                     AVATTVSLVFNTLFQLTLSSVTNMRTASFFTAVVEAPVVEECSKGAALLLIFFIAYLA
                     RRRSGLVEFAGVMDGIVYGSAVGFGFAIAEDLLYGLQFGPETYIVRRLFGGFAHASFT
                     SLTGIGIGLIPLVDNRVLKVILPVLGLSGAILLHATFNFTATVFGPVAYLVLFCVIIV
                     YIVIIIVWLAIERRAIRDELRDEVQAGTVTSQDYAMIPSYFRRTGYYLGLLFTGRLLT
                     WFRVRKLHSTAVDLAIAKRLARRTGSPGSIGRVRLLRRKILDLREGAAVRTVS"
     gene            16574..17143
                     /locus_tag="VN178_14095"
     CDS             16574..17143
                     /locus_tag="VN178_14095"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWS82146.1"
                     /translation="MAGGASRRMGREKLSLKVDGVPLIERVRNALTDCCREVLVIGGG
                     DVRLEGVRYITEERPGGLGPLSGMEAGLAYARYPFTFVAAGDMPFLTQSMVGYLLGRL
                     ARGGVLAVVPRYRDRIHPLCAAYARTLLPRLGTALDGGARAVHGFLEGVGEVEYVGDE
                     LGRFGNPDLLLMNVNSPGDLERARRGARR"
     gene            17140..17742
                     /locus_tag="VN178_14100"
     CDS             17140..17742
                     /locus_tag="VN178_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWS82147.1"
                     /translation="MKGQSRGGVLGELTELLRVYLGLPSILVPEGITARPRVAVVLGA
                     QVLQGGRPSTTLEARTRYAGELYDRGLADLLLPTGGEGEHQPAEAVVMSGILRGMGVP
                     EAAILREDRARSTWESALRVAEIAHHRGIGEVLVVTDPLHCVRTVASFGRAGLLAVAE
                     PVYSSPMWRKKWSRRGQFVRESGALVWYRIRHGVGSLSRP"
     gene            17745..18584
                     /locus_tag="VN178_14105"
     CDS             17745..18584
                     /locus_tag="VN178_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS82148.1"
                     /translation="MGVLAVSASAIFIRLADAPAIAIAFWRCALGAVVLLPPAFVRKD
                     SFPRGRALQVGIASGVALGAHFGFWISSLDYTSVAASVVLVSTQPVFVAVLAYLLFGE
                     RTTPLSFLGIVVAIAGTALIAFDRTVGSAAVLGNVLALVGAVTVAVYVLIGRSSRTTG
                     GVGVLPYSIVVYSAAAVTLLPVALLFEVRLWGYSGETWFWLAAITAGPQLMGHTVFNW
                     ALRYVQASVISGTILAEPVVAALLAWLLLSERPGLPTIVGGLVVLAGLYFLLKGRSAA
                     GEP"
     gene            18642..19316
                     /locus_tag="VN178_14110"
     CDS             18642..19316
                     /locus_tag="VN178_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82149.1"
                     /translation="MYGYPESVSGPIRTGYYYGLAGVLVALASDLYFLFVDPSTTPGW
                     VLAAIAQYRTRLALALFLFLAILAALRVRPTRLDPGVPYRSLLVRDGALAATVVAVMV
                     GITLFLTTALQATLLAGTARDYAREAAPRIVSYVNEVRTEIRQERKDRGAPPEKINDL
                     PPPARVAEVEATLQPPTLRELGRSIANLVLRAVLLGTVGGVVGALRGRPAPSNDEKPR
                     GAPRRE"
     gene            19313..20248
                     /gene="cbiB"
                     /locus_tag="VN178_14115"
     CDS             19313..20248
                     /gene="cbiB"
                     /locus_tag="VN178_14115"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HWS82150.1"
                     /translation="MSPQRGGQVATALLLDTVFGEPPEAIHPTVMMGLVISAFEKRAL
                     RIVGPRSRRLAGIVLAVLVPSLVFVTTRKILGVVPRKPRWMIGAALLSTTLSMRGLGG
                     AAGDVEHALREGRLEGARTRLGHFVGRDTVDLSESEICRAAVESVAENTSDGVVAPML
                     YGALLGAPGALAYKAVNTLDSMVGYRRPPYEDLGWASARLDDLANLAPARLTMLCTAA
                     TSGRPLGTLLTAVRYGPLTTSPNAGMAEAAFAGALGVRLGGANTYGGVLRVGPKLGDG
                     RLAAPEDIRRSVRLMRRCCVLAGTLLFLVERVFRG"
     gene            20241..21308
                     /gene="cobD"
                     /locus_tag="VN178_14120"
     CDS             20241..21308
                     /gene="cobD"
                     /locus_tag="VN178_14120"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HWS82151.1"
                     /translation="MVRGWAQHPGGRHHGAHGDLVSRSLGVRVGELLDFSQNINPLGA
                     PKDALEAARRALYEDSGRYPDLEYIGLREALAAYLGVGAEMVVPTNGGAEALFLAARA
                     AGTGGKALVLEPTFSEYAAAAAASGKEPVRRVARREEDFEWDSTPLRDLEGVSVVFLC
                     NPNNPTGDLLDRGAVLEVAARVVEAGAVLVVDEAFADFVPEISVTDMVDRGLWVARSF
                     TKFFAIPGLRLGCLVCDDAGRVQTLQPSWPVNSVAAAAGIAAAEDKGFAEASRAEVAR
                     LREDLLGALDAIPGLQPFPGAANFLLVRGPDGLPERLARRGVLVRGCGPFYGLGPEFF
                     RVAVRSAEENGRLLAAIREEL"
     gene            21326..21805
                     /locus_tag="VN178_14125"
     CDS             21326..21805
                     /locus_tag="VN178_14125"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWS82152.1"
                     /translation="MGGARSGKSALAERLASSMGGGRVLYVATAIVDGDEDLQRRIRL
                     HRERRPHGWGTLEIGDLDEVLEAADEWDAILLDSLTLWVSARGEPALPEFDRFLTRAG
                     ELGVPLFLVSDEVGLGVVPESEAGRRFRDLLGLVNQRAAEAAEEVHLCVAGMSIRLK"
     gene            21806..22540
                     /gene="cobS"
                     /locus_tag="VN178_14130"
     CDS             21806..22540
                     /gene="cobS"
                     /locus_tag="VN178_14130"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HWS82153.1"
                     /translation="MKALLSFFTVLPVRGATLEEAAREIHLLPLIGLVTGLPGAILLL
                     ATPVVPPGVAATLALGAVLLAAGLHHADGVLDVGDALMVHGSPDRRREVLQDARVGIG
                     GLGALFLVYAPTVGALAALCAVSPGRAALALFAAEVAVRSAMLLLLVFGEPADLRSSS
                     VPFVRALTGPHRTPAVIVALLAPLPFLLAMSALLAPLAILAVPLVALGLLRISAAKFG
                     GIGGDVVGATGEVCRAVVLIALSAMM"
     gene            22674..23288
                     /locus_tag="VN178_14135"
     CDS             22674..23288
                     /locus_tag="VN178_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HWS82154.1"
                     /translation="MSVEFLITSLVVVLIPGTGVLYTLSSSIGGGRRRGLFAAIGCTL
                     GIVPHLLAAMLGLSGIMQAGSAVFEVVRWAGVCYLVFVGATMIRDAGKISLDDVEAGP
                     DPMGPVVRRGILLNVLNPKLTLFFFAFLPQFLDASPGLHRLELIWLGGVFMLMTLAVF
                     GVYALASAAFRDLVLGAPVVRRWIERTLGAFLMAFAARLALTDR"
     gene            complement(23562..24278)
                     /locus_tag="VN178_14140"
     CDS             complement(23562..24278)
                     /locus_tag="VN178_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWS82155.1"
                     /translation="MLGGARITFLGHATFRFVTAGGEQIIIDPFLSENPQTPDDMKQV
                     GHLDTILITHGHFDHFSDVEPLAGQTGSATVSNFEIFSYLQRQGIENAMPIQKGGTVE
                     VGGLKVTATNAFHSSSIQLDDGSYIYGGEPMGYVIEFESGFKLYHAGDTAVFGDMRLI
                     GEIYNPDLALLPIGNQVVMSPLEAAHAARLLGVEHVVPMHYGTFPFLPGTPEEFQEHT
                     SALVPDLKVHVMKPGDDLAS"
     gene            24444..24866
                     /locus_tag="VN178_14145"
     CDS             24444..24866
                     /locus_tag="VN178_14145"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS82156.1"
                     /translation="MKSTLLLAVLAGVCVAVQTSLTGAAQRTLGPLVLVAISGLTTSM
                     FALSLSFMVAKPEFTGRAVVYAAASGVLGAVILGSIAVAAGQAGVARALSLVIATQLL
                     VGLVLDAFGVFGAGADFSVLKALGVLLIIAGGVLVVSY"
BASE COUNT         4187 a         7790 c         8285 g         4802 t
ORIGIN      
        1 ctcctactac gagcgcgtgc tcgacgtgtc cccggcgctc ggaaacacgg ccctcacgct
       61 gatgctcttc ggcggcgccg cagggaccct gattatgggt cccctggccg acaggttcgg
      121 caggcgcacg gtactactgg cctcgatgct ggtgctgccg cctctgatct tctgcttcac
      181 gctcgccggt cccactttcg gcatggcgct gctcatcctc gtcggggcgg ccaccgtggg
      241 gacattcggc gttaccgtcg ttatgggaca ggagtacctg ccagggcgaa tcgggctcgc
      301 ggccggcata acgatggggc tctccatcgg gctcggcggt gtcggcgcgc cgctgctcgg
      361 tctcatcgcg gataacgccg gcctctcctt cacgatgctc gttatagccg tattgcccgt
      421 gcccggactc gtcctcgccc tgaccctacc ccgcggaagg taggggctgt tagcgaactg
      481 cacggggtcg tgttcccgaa cacatcgctt ctgctcgggt cgggtaccat agctccaatg
      541 agggcgtcgt taagagaggg atatcagccg gggtgaacct attccatagc atcggtgtct
      601 tcgcctaccg gttccggtgg gcaatcttat tggtctgggg catggtcctg atctcgagcg
      661 ctttcttcgc cccagggctt tcgggacaac tcaagggcgg tgggttcgac ggtgcgaact
      721 cggaggccga gagggtacag gacttgatgt cggacgagtt cgggctctcg cctgctacct
      781 tgacggtggt ctttaccgga gacggtctcc ccgcccgcag cgaagagttc caggacgccc
      841 aggagagtgc gctagccgaa gtacgcaagc tggacgacgt acgccaggtc atctcctacg
      901 ccgacgctga agacccccgc tttatctcgg aggatggaat gaagtcctac gcggtagtca
      961 cctccaatgt ctccgtcgac gcgacgcgca acgtggtcga cgaggtgagg agtaaggtcc
     1021 gctccggcga gctcgagacc tacgtaacag gagcgccggc cgtctaccag gacctcgaag
     1081 aagccagcaa cgaggatgtg aaggaagccg agaaatacgc cttcccgttc gccgtgatca
     1141 ttctgatctt cgccttcggc acgctcgtcg ccgccggtgt gcccgtcctg atcgggggcg
     1201 cgagcgtggt gacggcgcta gccacactgt atttcatcgc tggcgtctac gatatgtccg
     1261 tgtttacgct tacgctttcg acgatgctcg gtctcgggct cggcatcgac tacgcgctgt
     1321 tctttgtgag tcgtttcagg gaggaactcg aggactaccc gaccgtggag gcggtgccgc
     1381 gcacggtggc gaccgcagga cggtccatct tcttctcggg gacggcggtg ttgatcggtc
     1441 tctccggcct tctgttcttc ccctttatgt tcatgcggtc gatcggggtg gccggcgtag
     1501 ttgtcgtctt cgtctccgtc ctggcggcgc tcacgcttct gccggccctc cttggtgtgc
     1561 tcggaccgcg gatcaacagg ctcgccatca ggcgtcccag ggatggggcg ggctcggcgt
     1621 tctggagccg cagcgccgag gtcgtgatgc gccgtcctct cgccgtgatc ttgctggcgg
     1681 ccgcgatcct gggcaccctg ctctacccgg tcggccacat gaaggtcggt atccccgagg
     1741 cgaccgtctt gccgcaggag tacgagtccc gcgccggaga cgacatcctg aagagtaact
     1801 tcgagtacgc cgcgctgaac ccgatggaga tcgtcgccac ccttgaggat gacccactca
     1861 gcgcccgcgg tctctccgac accagagagc tcggcgagaa catcagcggc gccgacggtg
     1921 tctcccgcgt cgagagcgtc tacacgctag cagctgctgc tgcggaggat tacgcggggc
     1981 gcctcgccga cgcccgcgag caggcgcgga agaaggccgc atcaagggtg gacgctatcg
     2041 tgcagcggca gatcgaagag cagacgcaga acgagacgga tagggccgtg aacgagcaac
     2101 tctctgagat agaggctgac tacggcgcag tacctccggg gaccgaggag cggctgcgga
     2161 cggatatacg gccgcgggtg gagagcgagg tgcgcgcctc ggagggccgc attagagatg
     2221 aggtcgagcg gcgcctggcc gaagaggtgg accgcaaggt gcccgacttg cccgagggga
     2281 tctcggccga cggtacggtt accccagagg gtgttgcgat cttcctgaac ttgccagcgg
     2341 ccaggcgatc cgaggacctg caagacaccc tcgacacgta cgtggcggga gatagaacac
     2401 tgcttcgggg ggtgaccgag gcgaacccct atacacaggg cgcctacggc gcggtcgatg
     2461 ccgtgcgcgc ggtcgaggcg ccggatggaa cgagcttcct agtcggcggg ctctccgccg
     2521 gccagaaaga cttcatctcc agcctgtacg gcaaagcgcc cctggctgcc gccttcgtta
     2581 tcggagtgac gtacctgatc ctgctcctca ccttccgctc ggtattactc ccgctgaagg
     2641 cggtcctcgt caacatcctg agcctcacgg cgagcttcgg ggcattggtc ttcgtattcc
     2701 aggacggcaa tctctccggc ctcctcgact tcacccctct aggcttcgtg gatgcgacgc
     2761 ttccgatcct gatgttttgt acgatcttcg gggtctctat ggactacgaa gtgtttctcc
     2821 tctcccgcat aagagaagcc tacgagaacg gagattccaa cgccgccagc gtctccaagg
     2881 ggttggtggc gaccgcggga atcatcacct cggccgccgc gatcatcata gtggttaccg
     2941 gggctttcgc cctgactggc atcgtcctta ccaaagccat cggcctcggc ctcgccgtcg
     3001 cagtgttcgt cgacgcgacg atcgtccgta tcctcctcgt ccccgccacc atgcgcattt
     3061 tgggtgattg gaactggtgg ccgggtggac gcaaggtcac tttcggggcc gacccggaag
     3121 agattgcaaa ggactggccg ccggataggg aatccccgag gtaagatcga ttcttgatct
     3181 tgctgtgtgc cggggataga ctgtggtgga tgctggaatt cgagcggata ctcaaggaca
     3241 ggggctacag gctaaccaac cagcggcgga taatcgtcca tgagctggat ggggagcggc
     3301 acctttcggc ggaagagatc tacgaccggg tcaagggcga gcatccggag cttggcctct
     3361 ccacggtgta ccgcacgctc gaccttctgg tcgaactcgg catcgtacgc aaggatgact
     3421 tcggggaggg ctactcgcgt tacgagctgg cgacggaacg gatgcaccac cacgcgcgct
     3481 gcgagcagtg cggcaacgtc atcgaattca acgaagagct gatggagtat ctggccctgc
     3541 aggtcgaacg ggagaccggt ttcgtcacgg attggcacga gatcaccctg tacggccgct
     3601 gcgccgattg ctcggagtaa tccaagtccg gaaaaagaga gaggcatgtc aggcagccac
     3661 gagatcgctt cgtatccgac aaggactcca aaggacgtgg attcccgctt gcgcgggaat
     3721 gacgcttagg gatgcctaac cgatggtgat tggacgtact tggtaaaagg cttcaggatt
     3781 tggatcggta gaggaggtgt accgccttaa tcctggggcc tgtaacctga agccgtgctt
     3841 cgcgaaacgc tctagacctc gagctccaga gcgtcctcgg cgacgagtat ctcgccttcg
     3901 tagccccttc ccacttcggt gacgaggtct agatcatccg ccgccgggta gatgtgggtt
     3961 aggacgacac gacgtacgcc tgcctcgctg gcgatacgtg ctacgcctga aggcgtcagg
     4021 tggccgggaa cgggggtttc gtcggggaag gaacactcga tcaggagcgt atctgcgccc
     4081 cgcgccagtt cgactacgga cccactgtat tcggtgtcgc ccgtgtatac gaatgcgccg
     4141 ccttcggcat ccagcctgta ggcgatgctc tccctgcgat gctccgccgg tgcccacgag
     4201 agcctgcatc cggcgaggtc gatgggggat gggcactcct gggatagctc cgagaggagc
     4261 gtggcgtggt cgccggacat ccactttccc cagacttcca tcacggagcc ccagaaccgt
     4321 aagaagggct cgggaccgct caggtagagc ggcagggtac gtttctcggg tgcgccgtag
     4381 ttgatggcga agagtagggg aacgacgtct accgtatggt ctggatggaa gtgcgtaaag
     4441 aagatgcggt ctacggcgaa ggggtcaagg cccgactcga gcatgccgcg gaccgcgccc
     4501 gaaccgaggt cgaagacgag cgtctgccca ccggcgcgta ccacgacgca gctctggcgc
     4561 ctctcgaggc gtggaactac ggtacccgat cctacgacgg tgatcttcat gttcctccct
     4621 ctggtcggaa gctttcagcc ctcaactttc agctgtcagc cttgtggcga cggatcggtc
     4681 gctcattcgc gttgcatcga ttttcggcct gacggcacgt caagtttgat cgtctcccca
     4741 tggtgggtag ctcatgggtc cttggctgat ggctgcccgc tgaaagctga ctgcttttca
     4801 aacgtccagg cgagtccggc gaggccgagc agggcgccac cgaggacgtc ggagggccag
     4861 tgcgttccga ggtagaccct gctcacagcc gatgcggcta ggaagatcaa gagtgcgatc
     4921 ctggccggtc tgctcggcca caaaaggtag atggccccga gcaggaggac cgagcgcagc
     4981 atatgcccgc tggggtaggg gtatggcgta ctcacgtcga gcaacgaggg gtccggcgtt
     5041 cgcatggcat cctcgggtac gggaggctgg ggtaccatcg tttttgctgc gagctcgatc
     5101 agtcccgtaa ccaggaaggc tatgacgagg cgtagggcga gagggcccct tccgaggagt
     5161 agcagactgg ttgcgagcgc gactgtggcg acggcgacga actcgacccc gccagccacg
     5221 gaggttacca acccgatcgc atcgaggacc cctgtgtctg ttctctgggc cgcgtcgagt
     5281 atcaggacgt cgagaggttg gaacagccct gcgccggcca cgaccgagca agctaggaat
     5341 gccgcggtcg agagcagcag gtacatcctc ttgtcgccct tcacggcgtc atagactacc
     5401 aaagcgggaa gccaaaagcg caggaccgcg cttcttctcg tttctgacgc agcaaaaacg
     5461 aactagagcg cgagtggcat gcggcgaaaa caagaagctg agagctgaaa gcggtagcaa
     5521 agtggttaaa ccactttgct accttcgcta tgctacggcg tcgcgcagga cgcggccgta
     5581 ctctccacct ttcaggatac gttcagcctc tcgctggagc tgcctgactc gctcgcgcga
     5641 aatgtccagc tcgtcgccga gttcggcgag ggtggccggc tcgcggtcgt cgagaccgta
     5701 gcgcctgacg agtacgtagc gcgccctctc ggggagacct tcgatcgcct ctttgagatg
     5761 ctccgtctcc atctcgcgca tcacggtatc gggcgtatcc gagaccttat cgtcctcgat
     5821 gaagtcaccc agctgggagg cggtctcctc gctggatact ggctgatcca ggctcgtcgc
     5881 gtccggcatg gcgcgcatgg tgaggcgcac ctcgtcgggg tcccagtcga ggcgcctggc
     5941 gacctcttcc tcggtgggct cgcgttcgat ctccagggcc agctcgctca tggcgcggct
     6001 gaccttcctg atcttctccg tcatgtgcac ggggacgcgg atggtgcggc ccttgtccgc
     6061 cacggcacgc tgcacggctt gacggatcca ccaggtcgcg tacgtggaga acctgaagcc
     6121 cctgtcgggg tcgaacttct cgacggcctt catcaggccg atgttgccct cctggataag
     6181 gtcctcgaag gggagcccgt agccgcggta ctttttggca acgctgacca cgagcctcag
     6241 gttcttctcg atgagccgct gcctggcccg tttgtcgccg gccttggccc tcacgctgag
     6301 gtctatctct tcggcgtgcg tcaggagatt gccctgcccg atgtgcgcga gatacttggc
     6361 gaggagttcg ggagtctccg attcccgctc gattttgtga ttttccttcc gcttctcgaa
     6421 actggccttc aaaataccct cctcactaca cacccatctt acccgaaatc agttctggaa
     6481 actactattg gttctaccgc gaggtttaat tccctaaacc tctccgaggg ctctctcctc
     6541 accaggtctc actaccgctt gccactacac tgatacatac gtatgtcgac gcgaaatagt
     6601 tgcatgtggc ccgcttcgtc gtcgcgttac ggtgtcttgc ggcgggcgcg gttatacttg
     6661 ggctcatgga gaatgcctcc cgcatcccgg accgcataag cgaggacatc gcgaacatcg
     6721 tggcctgctg gcaggcgcgc aggcgtggag acaacgagga cggggtctat gatcgcatgc
     6781 tcgccggtat ggaggaattg gtctcggtat tcgtcgcatt tctacgcagt acggagacgg
     6841 tcgagacctt ttcccggggt ggggcgacca gggctatggt ccgcaggatc gccgagtacc
     6901 agcacgattt gggcagggac gcggtagggg tcatagaaga tttcagcgtc ctgcgacggt
     6961 gcatctggcg ctcggtcgag gctggggtcg acctcggcgg gctgcatggg ggggaggtct
     7021 cggggttttt cgccaagctg atgcaggctt cggactggct taccgaggcc ggactggagg
     7081 ccttcgacgc catagtgcgg cgcgatatgg agcaggctct cgggcgggct accgccaccg
     7141 acctggtgac cgggctgccc gacagagatc aactaaaccg gctacttctg ccacgggcca
     7201 tagattcgta tgagaggttc tccgtggccg tcttcgacgt cgccggcttt accgagaccg
     7261 tcgcggcggg gaagatcaag cgcgccagga aggtcctgcg cacgctagcc gaggccgtgc
     7321 aggcgtcctc gccgcagggc tcccttttcg cccgcttcgg ggacgacgag gtctgcgtga
     7381 tcctgcccgg cgagagcggt gaaggtggct accggttggc ggagcgggtg ctcggacgcc
     7441 tggtggaggg catggcagag ttcgaagtgg acgtaggtgt cgccgagtat ccggcacacg
     7501 gaacggatcc gggggagttg atgggggaga ccctcaaggc gctcaagatg gcccaacgcg
     7561 tcggtggcgg cggtatcgtg gtagcccgct aggtagttgg gaggtatacg tgatcgcgat
     7621 cttcaacagc ctgcccgtca aagaaggagc cgcagacgag atcgtgaacc gctttgccga
     7681 aagccgcggg tacgtacagg ggttcccagg gttcgtctcc atggaggttc tcgagtccga
     7741 tgcgggggac gaggtgctcg tcgttacgcg ctggcgggat cgggaatcgt tcgaggcttg
     7801 gattgacagc gatgcgttct ccagggcgca tggccgcagc ggagcgggcg aactactgac
     7861 gggccacccc aggatgacgt cctatcgggt cgcggtcgag cgtgcgccgg aggcttccgg
     7921 cccgggttga ggatcaggcc aggctttgag agagcagctg gggcttctct ccggtcggtt
     7981 catactcacc cttcccgagt acggcgaagg taaaggcgaa caggcagatc agtagcagga
     8041 tcaggatccc gaatgccagc cgctcccgca ccttgtctcc tctctcgagt acgaacctca
     8101 gcgccattct aacccaatca acgaggcacg tcctgcgaat ttctctcatg ttcgcccgct
     8161 cgtaatgtac aatgtagcaa tgtgtagcac gctcagcccc gaggttcgcg agtatcggtg
     8221 tggttctggg gcggaatcgc agggaggaga cgcacgtggc cgttgatatt tccctgaccg
     8281 agtacacgaa actgcaggag caggagtgcc ggtcgctctt gcggctgctc gacgagatgg
     8341 gcatcgccac ggccgacagg gtctatggac cgggggacgc catctacaac gagggggagt
     8401 acggtgacgc cctctacgtg ctgacctcgg gggtactgaa gctgttcagg ccctactcgg
     8461 gtagcaagga ggctacgtta cgattgctca agccgtggga tatcttcggc catctggcct
     8521 tcgccgggga gaccaggcaa cgcgcttacg ccgaggccgt tacggagtgc agggtgacca
     8581 aggtacccaa gatcttcgtc gaacgcgccg tacgccagga accgcaggcc gccttcaagc
     8641 tcatgaccct tctggagttg cgtctcgtgc agtacgagga gctagtccag tgtctgttgc
     8701 cccgcgagac ggaggtgcgg ctcgcgaatc tgctgcccct tttggcgcag aagttcggcc
     8761 agagcaggga cggcctcgtg acgataggcc ttcgtttgac ccaccaggac ctcgccgcca
     8821 tggtcgcctc caccagggag tcggtcacca aggtcctcaa cgagatgcgt aaccgcgacg
     8881 tgatcgaggt cgaggcgggg cgcatcaccc tcaaagaccc cgcagccctc gccgtgctaa
     8941 gcggaatcta gaggccacgc taccctccag tagggttcct ccgaaacgaa acgtatacct
     9001 ggcctgtcgg gtagcccccg gtcagccgct ggaaaccgga cgcggatagg ctaatatctc
     9061 cgcgtgctgg agctggttca agcgatactt ctcggggtgg tgcaggggct cacggagttc
     9121 ctgccggtct ccagctcggg ccatctgtta ctcgggcaat actttctggg catggatcag
     9181 gaccgcttcg ggcttacgtt cgacgcctcg atccacacgg gtacagtgct ggccgtcgtg
     9241 gtgtattttt ggcgggatct catgcgtatg gcggccgcct tcgtggcttc gttgcgaggt
     9301 ccggatcttg cggaccggga ccagcgcatg gtgtacttga tcctggttgc ctgcatcccg
     9361 gcgggcgtcg tcggactctt gttccagaac ttcttcgagg aggaggtgcg ctcgccttgg
     9421 ctcgtcgcgt tcaacctggt gcttgtgggc gcgctcttca tcgtgggcga ggtcgtgggc
     9481 cggcagaccc gcacctccga caagttgggc ttcaaggagg cgctcggcat aggtctggcc
     9541 cagacggcgg ccctgttccc gggcgtctcg cgctcggggg cgacgatcac gatcggcctc
     9601 tttctgggcc ttcggcggga cgaggcggcg cgtttctctt tcctgatgag cgtccccatc
     9661 acgaccgctg ccgcgctctt gagcctggtc gacgccttcg gatcggggat cgactccaac
     9721 gacgtgttgc tcttcctggt cggcagcatc acctccgggg tagtcgggta cctggccatt
     9781 aggtttctgc tcaattacct ctccgggcac agcctgctcg tcttcgccta ctaccggttc
     9841 gcgctggccg ccgtggtggt cgtgctcctt ttgttcggcc tcgggggctt ttgacaggcg
     9901 ccgcgctctt ctggtagttt gaggccgata tgattctcgc ttacggtgcg ttgcggcgat
     9961 ttaccgggga ggggcgtgcc cggcccgcgg ccatgctcct cctaccctga gcccgaaccc
    10021 ggcacctacc gaaggacgca tacccatgtt cccacccgac gagaacacca cccacggccc
    10081 tcaggctccc gactcgccgg acgagatgca taccctgcgc gaaagcatag actcggtgga
    10141 cgaggagatc gtgcgtctgc tcgacaggcg tgcctggctg gcgcggagga taggtgagat
    10201 aaagcacaag aacgggctgg cggcctacgc acctgccaga gagcgggtcg tcctcgaccg
    10261 tgtcgcgacc ctcagcgatg gggagttccc caggcgcgga ctgcaggccg ttttccgcga
    10321 gatcatctca tcctccatct cgctcgaggc cagcctcaag gtcgcttacc tcgggcccga
    10381 ggcgacgttc acgcacgagg ccgccctgcg ttcgttcggc accagcatcg agctcgaacc
    10441 ccagacgacg gtcgccgagg tcttcgccag ggtcgaacgc ggggaggtcg aacacggcgt
    10501 cgtgcccgtc gagaactcga tggagggcgc cgtgacccac acgcttgacg agctgatgaa
    10561 cagcccactg aagatctgcg gggaggtgta cctgcccgtc tcccagaatt tgatctccgc
    10621 ggaacagtcg atagagaggg tgagcctggt gtgctcgcat cccatggccc tggcccaggc
    10681 cgcgagctgg ctcaggcgcg agctccccgc agcccgcctg gatgaggtcg attccacggc
    10741 ggaggccgcc cggcgggcgt ccctcgagcc tggtgtggcc gccatcggaa gcgtgctggc
    10801 ggcgggagcc cacggtctga cggtgctggc gcgcaatatc caggatgccc gcgcgaacac
    10861 cacgcgcttt atcgtgctgg gccgcgcctg ggccgcgcgg accggcaagg acaagaccag
    10921 cgtcgtattc tccgtcaagg atcgccccgg cgttctgaag gacgcgctct cggccttcgc
    10981 ggaggagggg atcaacctta cccgcatcga gagccgcccg agccgcaagc gtgcctggac
    11041 ctacgtattc ttcgcggact tcctcggtca ccccgaggaa gacagggtga agcgcgccct
    11101 cgaagccctc gaggaacact gcccatacgt cagcctgatc ggggcctacc ccgaagtctc
    11161 ccccgaagta gtctgacggc ctcagactgc gtggacttca aggccgccgc gcgggaggtt
    11221 ctcgaggagg tcggccatcc tctccactac acggacataa cggagatcgc gctccagtcg
    11281 ggttatctgc agagctcggg ccgtacgccc cagaatacga tgagggcgag gctctccgtt
    11341 gatgtcaggg acaacccgaa tacgtacttc gcgcaggtcg cgcccggcgt ctacggactg
    11401 aaatccaatc cgggtggagg cctcgactct tcggtcggta gggaatagct tcagtgctga
    11461 gatactatac tgcgaggaac gattctttga cagccgagga gggaatatgg tcgagttagc
    11521 ggataaggtg agcgaagcgg cctggaggat caagaacctg aaggactacg acgagcggct
    11581 ggagagggcg gcgcgcaggt tcgacgagat atccagggag cgccccgagc ccgcgctggt
    11641 gatctacgcg agcgcgctca agaagagcgg acgtagtgag gatgagatcc gcggcatggt
    11701 ccgcgagttt cacgagactt tcgaagacga agaagagccg ctgaccataa cccacgcgag
    11761 tggcatcatg agcgtcgagg gcgacgactg gttcttcggg gacgcggacc tccgcgtcct
    11821 gacgggcagg ctgctggggc agttccagca cagggttgcc cagtacaact acgtcgacga
    11881 gagcgaggtc ctgaggcgct gggccgacgg ctacgacacg cgcctcttcg tgaggcggcg
    11941 catccacgag acggagcccg tcgtcggcgt ggtgggggac ctggatctta cgctcttcca
    12001 gtacctgcgc gtcgcggccg gcggcaatac cctcgtaccc accgagaacg tctccagggc
    12061 gctcggcgcc ctcggctacg agggtgacga atacgagacc ctggcccaag cggagggact
    12121 ggccctttac ctcgatcttc ccgctccgat cgtcggggag atgctagagg atctcggccg
    12181 cgaaggtttc accgatttcc cccaaccgcc gtccgggtcc ggggagaacg atggagaagg
    12241 cgttgctccg gagggggact ccgaggagaa tgaagagaag gcctaggacc gaacgccgcc
    12301 agcaagccag gagaaatggt agaatacccg gaagtattct caacaataac tcgtcaagca
    12361 acggctcagc gcagcatcta ccagaacggg ttctaagaag gagcaggcgg cagcatggca
    12421 gactccagaa agcgcatagc gttgaagccc cacatggacc gtatcaggga gtgggtggag
    12481 acgggcaaga cggacgactg gatcgcagac gccctaggca ccagcgcatc ctcggtacag
    12541 tccttccgtt cacgcaacga catctaccgg cgcgagcgcg ggcctcgcga gcccgagagc
    12601 gtcttcgagg gagtcctcga ccacggcgaa cgcgacggtt ggggcctgtg gctcgatcct
    12661 tccatcgctg aggacccgat ctggaaggag cactgggcag gggtggaggc cgtcgtcgtg
    12721 aaggtggccg aagactccat cgtgctcgag gccgacgggg gagagtcagc gaccgcagac
    12781 gacctcgcga tgcctgttac tatccccgac gggacgtccg atgaacaggg gtccgggctg
    12841 ccgcgcgagc gggggcgcgt caagtggttc gacgcagaga aaggttacgg tttcctggta
    12901 cgacccacgg gcgatgatct tttcgtccac cattctgagg tacagggtga tccctcggaa
    12961 ctctccccca gcggggaagt agagtacgaa gtcggtcgta acgaccgggg tccgaacgcc
    13021 cgaagcgtca ggccgttggg ctagcgtagg gggggaggtt tgaaacctct ccctactctg
    13081 ttgtggaggg gcctgggtct tcgctcaggt gcgctgtcga ggctacctcg agcaggcgcg
    13141 gtcccatctc accccattgc aggcgtgtga tcgaagcgtt ggccgcccgt tgggcgccgg
    13201 gcatgacctc tggtccgaaa agttccctca gatacgcccg cattactcct ccgtgcgaga
    13261 cgacgacgat gcgcgaaccg cgggggtgga gggcgcggat agacgagagg gcctcttcga
    13321 acctcgcgat gatctcctcg tccgtctcgg cgccgacgag cgcccagcgt tcctcctcgg
    13381 ggatcgcgag gaacttcttc gccagctcgg ggttgcgctg ctcctgctcg gcccaggtgt
    13441 ggccttcgag gataccgcca ctcctctcgc tcaggccgtc gacggggacg acctcgcccg
    13501 cgaacctcgt acgatcgcgg atgatctctg cggtctggaa agcacgggag agcgggctcg
    13561 agtagaggcc gtcgaacggc tcgccggcga ggccgagtcc tgccaggcca gcctgcaccc
    13621 tgcccctctc cgagagtggg aactccatct ggccctgcca gatgcctctc gcgttcgccg
    13681 tggactgtcc gtgacgaatt aggagcaact cttgtggttc tggcatcggg taagggtagt
    13741 gtatagaggc gcttccggca aatcttcgtc gatgctagaa tctgcgggtt cgtggcttga
    13801 tgggaggctt accgggatga tccggagaga cggagaacag acccccgaag aaggtgtgga
    13861 agcggttcag gaagagaata cggatcccat acagatcgag cgcgacgccc ctcgtcctgt
    13921 cacgatcctc agggtggccg gagaattgga agagcggggg ggcacgatcc tcgagctctt
    13981 caaagagatc gagtctcccc tcggacgggt gatccttcct atatacctgc gccaggacga
    14041 cagggatttc ttcgtcgagg tcgcgaccgg accgtgggac gaccggaagt ccggcgaggc
    14101 gctcgacaag gccgctattt tgcggagctc ggagcacgcc ggcgcgggct tggagatact
    14161 gagcggctac cccgtgcccc ccgaggtcga gttctacttc ggcacctcgc cggccgcact
    14221 cctgcagctc gacctcgcgc ggctcacacc ggaacggccc gaggtgtgcg ctggcctctt
    14281 ccgggaggtc gggagcaacc actggggcgt cgacctcgac tacgagccgg ggtacctgcc
    14341 gctcatcgaa gagctactga tggccgccct cgaaggcgac gaagcccaca acagtacgcc
    14401 cctctccggc gggctcgtgt ccgggctcgg atgttttctg ggcgagatca tccgccgtaa
    14461 cgtcagccca cccggggtgt ggcgcccgcc cgaggagtgg ggggaaggac ccgtcgtcga
    14521 gatcggggac ttcgtcctcg accccgtggg taaagccagg gctttcctcg agtacgggcc
    14581 cgaggagtcc ttgactctct acgccgagta cgtcctcgaa aggcgggacg ggggtgagct
    14641 cccccccgac cttcaggcgt agcggttgcg gagctcgcgg attatgtgcg aggaatcgac
    14701 gaggacctcg tcgcctatgg tgaggatcgg gatcgcccgc tgtcccgaga gcctgatgac
    14761 ctcctcacgc atgtctgcgt cggcgtctac ggactcgtag tcgagcccga gccgctccag
    14821 ttcatttctc acccgacggc agaacgggca gtagtcgccc gtatataatc ttatgcctgt
    14881 cttctgtaag gtcgcatcca tagcaagcct ccagactatc aattcgttct ctcgaatgta
    14941 ttgtaccccg agacgttgga tcctacgccg atcggtgtcc gtctacctga gggcctcttc
    15001 gtactcggcg gggtcgtaga tcagggggta gctctcgtcc agggtgtcgc tggccgttac
    15061 ctcgccgacg accacggtgt ggtctcccgc gcgggccctg tcgatgacct tgcagtccag
    15121 gtaggccagg cagtcgagta ggaaaggcga gcccccaggc gtgaggccgt aggggatgcc
    15181 ctggagctta tcgtggtact cgccgctcgt gtccgcgaag tgggtcgcga cgtctacttg
    15241 atcgtcgcgc aggacgctga gggcgaaggt ctcgctctcc agcacgatgt cgtgcgtgta
    15301 acggtcgctc ctgacggcga ccgccaggca cggggggcgt tcggaggtct gcatcaccca
    15361 cgatgccgtc atggcgttgg actggcggcc acgccgggtt cccatcacgt agaccccgtg
    15421 ggtcaggtga cgcaggccgg ccagtataga atcgcggtct cggagatccc tgtccaatcc
    15481 actcctctcg gtaaggacaa gggtatttta tcaggagcgg gtatactgcg gtccatgtca
    15541 cactcgggaa agccaacctc gtgggcctga gcggtacgct gatctggctc ggcatcctgc
    15601 aggccgtgat ctacctcctc ttcatccggg ctatagacct ctacgagcgg gaaccgctgc
    15661 gctacgtgat accagtcttc atctggggat tcgccgtggc gacgacggtc tcgctggtct
    15721 tcaacacgct cttccagctc acgctcagct cggtgacgaa catgaggacc gcaagcttct
    15781 tcaccgcggt cgtcgaggcg cccgttgtgg aggagtgctc caagggggcc gccctgctct
    15841 tgatcttctt tatcgcctac ctcgcgcgcc gccggagcgg gttggtcgag ttcgccggcg
    15901 tgatggacgg tatcgtctat ggctcggccg tcggtttcgg cttcgccatc gccgaggacc
    15961 tgctctacgg tctacagttc ggtccggaga cgtacatcgt tcgcaggctc ttcggcggct
    16021 tcgcccacgc gtctttcacc tcgcttacgg ggatcggcat cggcctcatc cccttggtgg
    16081 acaacagggt tctcaaggtc atcctgcccg tcctgggtct atctggggcg atccttttgc
    16141 acgcgacttt caactttacg gccacggtgt tcgggcccgt ggcctacctg gtgttgttct
    16201 gcgtaatcat cgtttacatc gtgatcatca tcgtgtggct cgccatcgaa cggcgcgcta
    16261 tccgtgacga actcagggat gaggtccagg ccgggacggt aacctcgcaa gattacgcca
    16321 tgatccccag ctacttccga aggacgggct actaccttgg gctactcttt acggggaggc
    16381 tgctcacttg gttcagggtc cgcaagctgc actctaccgc cgtcgacctt gccatcgcaa
    16441 aacgcctcgc ccgccgcacg ggtagcccgg gcagcatagg ccgcgtcagg ttactccgtc
    16501 gcaagatcct cgacttgaga gagggagcgg ccgtccggac ggtctcctga gccgtccggc
    16561 ctcgggcata atcctggccg gcggcgccag ccgcaggatg ggacgcgaga agctgtccct
    16621 gaaggtcgac ggggtgcccc tgatcgaacg cgtcaggaac gcgcttacag actgctgcag
    16681 ggaagtgctg gtaatcggag gcggcgatgt caggctcgaa ggagtcagat acatcaccga
    16741 agaacgccct ggcggactcg gaccactgtc cggcatggag gccggtctcg cttacgctcg
    16801 ctacccattt accttcgtcg cggctggtga catgcctttc ttgacgcaga gcatggtagg
    16861 ttacctgctc ggccgtctcg cgaggggcgg tgttctcgct gtggtcccac gataccggga
    16921 cagaatccac cccctttgcg ccgcctacgc caggacgctg ctaccacgcc tcgggacggc
    16981 gctcgacggc ggggcgcggg ccgtgcacgg gtttctggag ggtgtcggcg aggtcgagta
    17041 cgtaggagac gagttaggac gtttcgggaa ccctgacctc ctgctgatga acgtcaactc
    17101 gccaggagat ctggagcggg caaggcgcgg ggcccggcgt tgaagggtca atctagggga
    17161 ggtgtcctcg gggaactaac cgaactcctg agggtgtatc tgggcctgcc atcgatcctg
    17221 gtgcctgagg gcataacggc caggccccgg gtcgccgtgg tcttgggggc gcaggtactt
    17281 caaggaggga ggccgagcac tacgctggag gcccggaccc ggtatgcagg cgagttgtac
    17341 gatcggggcc tggccgatct tcttctaccg accggcggcg agggcgaaca tcagcctgcc
    17401 gaggccgtcg tgatgtcggg catcctgcgg gggatgggtg tgccagaggc ggcgatcctg
    17461 cgcgaggata gagccaggag cacctgggaa tccgccttga gggtggccga gatcgcgcac
    17521 cacaggggca tcggcgaggt ccttgtggtg acagaccctt tgcactgcgt caggacggtc
    17581 gcctccttcg gtagggcggg gctgctcgcc gtagcggagc cggtgtatag tagcccgatg
    17641 tggcgcaaga agtggtcccg taggggacag ttcgtcaggg aaagcggggc tctggtctgg
    17701 tacaggataa ggcatggggt agggtcgctc tcccggccgt agccgtgggg gtcctcgccg
    17761 tatcggcgtc cgcaatcttt atccggctgg ccgacgcccc tgccatcgcc atagcgttct
    17821 ggaggtgtgc cctcggggcc gtcgtgctcc tgccgccggc gtttgtcagg aaggacagct
    17881 tcccgagggg ccgtgcgctc caagtgggga tagcctcagg ggtggcgctc ggggcccatt
    17941 tcgggttctg gatctcctcc ctcgattaca ccagcgtggc cgcgagcgtg gttctcgtgt
    18001 ccacccagcc cgtcttcgtg gccgtcctgg cgtacctgct cttcggggag cggaccaccc
    18061 cgctatcgtt ccttggaatc gtggtcgcca tagcggggac ggcccttatc gccttcgaca
    18121 ggaccgtagg atcggccgcc gtgttgggga acgtgctcgc cctggtgggg gccgtgacgg
    18181 tagctgtgta cgtcctgatc ggtcgctcct cccgcaccac ggggggcgtc ggcgtgctcc
    18241 cttactccat cgtcgtttac tctgcggctg cggttacgct gctgcccgtc gccctcctct
    18301 tcgaggtcag gctctggggt tactccggag agacctggtt ctggctggcg gcgatcaccg
    18361 ccgggccgca gcttatgggc cacacggtct tcaactgggc cctgcgctac gtgcaggcct
    18421 ccgtcatctc gggcaccatc ctggccgaac ccgtcgtcgc cgcgctcctg gcctggctcc
    18481 tcctctccga gagaccgggc ctgcccacga tcgtaggagg cctggtagtg ctcgccggtc
    18541 tgtattttct cctcaagggg cgttcggccg cgggcgagcc ctgacaggcg tgggattctg
    18601 gatcacagag ggtcccctgg cgatgctgta tcctcccctg tatgtacggt tacccagaga
    18661 gcgtttcggg accgataagg acgggttact actacgggct cgcgggggtg ctggtcgcgc
    18721 tcgcctcgga cctgtatttc ctcttcgtcg acccctccac cacgcctggt tgggttctcg
    18781 ccgcgatagc gcagtacagg acacggctgg cccttgctct gtttctgttc ctggcgatac
    18841 tggcggccct cagggttcgc cccacacgcc tcgacccggg tgtgccctac aggtcgttgc
    18901 tggtacgcga cggcgccctg gcggcgacgg tggtcgccgt tatggtgggg atcacactgt
    18961 tcttgacgac cgctttgcag gcgaccttgc tcgcagggac cgcccgcgac tacgcccgtg
    19021 aggccgcccc caggatcgtc tcctacgtaa acgaggtgcg tacggagatc cggcaggaac
    19081 gcaaggaccg cggggcccca cccgagaaga tcaacgacct cccaccgccg gcgcgagtcg
    19141 cagaggtcga agcgaccctg caaccaccca cgttgcggga gctcggacgt tcgatcgcca
    19201 acctggttct gcgcgccgtc ctcctcggaa cggtgggcgg ggtggtcggt gcgctccgtg
    19261 gccgtcctgc tccctcgaac gatgagaagc cccgaggggc gccacgcagg gaatgagccc
    19321 gcaaagagga ggacaggtcg ctacggcgct gcttctcgac accgtgttcg gcgagcctcc
    19381 agaggcaata cacccgaccg tgatgatggg actcgtgatc tcggcgttcg agaagcgggc
    19441 gctgagaata gtgggcccga gatcccggcg cctggctggc atcgtgctag ccgtcttggt
    19501 gccttcactg gtgttcgtca ccacgcggaa gatcctgggc gtcgtgcccc gcaagccaag
    19561 gtggatgatc ggggctgctc ttctctctac cacgctctcg atgcgcggcc tcggtggggc
    19621 cgcgggggac gtcgagcacg cgctgcgtga aggacgactc gaaggagcgc gaacgcggct
    19681 gggccacttc gtcggacggg acacggtaga tctttcggag tccgagatct gcagggccgc
    19741 cgtggagtcg gtcgcggaga acacgagcga cggtgtcgtg gcccccatgt tatacggcgc
    19801 gctcctcggt gcgcctggcg cgctggcata caaggccgtg aatacgctgg actcgatggt
    19861 cggctacagg cgaccccctt acgaagacct cggatgggcc tctgcccgtc tggacgatct
    19921 cgccaacttg gcgccggcca gacttaccat gctctgtacc gcggccacct cgggacggcc
    19981 ccttggcacg ctcctgaccg ccgttcgata cggaccgcta accaccagcc ccaacgccgg
    20041 gatggccgag gccgcattcg ccggcgccct cggcgtcagg ctcggcggcg ccaacacgta
    20101 cggcggggtg ctgagggtag gacccaaact cggcgatgga cgcctggccg cgcccgaaga
    20161 tatccggcgc tcggtgcgcc tgatgcgccg ctgctgtgtg ctggccggaa ccttgctgtt
    20221 tctggtggag agggtgtttc gtggttaggg gatgggcgca gcacccgggc ggccgtcacc
    20281 acggtgccca cggcgacctc gtctcgcggt ccctcggcgt ccgtgtcggg gagctactcg
    20341 acttcagcca gaacataaac cccctcggcg cgccgaaaga cgctctggag gccgcccggc
    20401 gggccctcta cgaggattcg ggtcgctacc ccgacctgga atatatcggg ttgcgcgagg
    20461 ccctggccgc ctacctcggc gtcggtgccg agatggtagt gccgaccaac ggcggcgcgg
    20521 aggcgctgtt cctcgcggcc agggcggcag gaacgggcgg aaaggcgctc gtgctcgagc
    20581 ccaccttctc ggagtacgcg gcggcggcag cggcctcggg caaagagccg gtccgcaggg
    20641 tggcccggcg cgaagaagat ttcgagtggg actccacccc gctcagagac ctcgaaggcg
    20701 tctctgtggt attcctctgc aacccgaaca accctacggg cgacctgctc gaccgcggcg
    20761 cagtactcga ggtggcggcc cgcgtggtgg aggccggagc cgttctcgtc gtcgatgagg
    20821 ccttcgccga cttcgtccct gagatcagcg tcacggacat ggtcgaccgg gggctctggg
    20881 tcgcgcggtc cttcaccaag ttcttcgcga tacccggcct gaggctcggc tgtctggtct
    20941 gcgacgatgc cgggcgcgtc caaaccctgc agccttcctg gccggtgaac tcggtcgccg
    21001 ccgccgcggg gatcgccgcc gccgaggata agggcttcgc cgaagcctcc agggccgagg
    21061 tggcccgcct gcgcgaagac ctcctcgggg cgctcgatgc catccccggc ttacaaccct
    21121 tccccggtgc tgcgaacttc ctgctcgtcc gcggacccga tggactcccg gagcggctcg
    21181 ccaggcgggg tgtgctcgtg cggggttgcg ggcctttcta tgggctcggg cccgagttct
    21241 ttcgagtcgc agtcaggagt gcggaggaga atgggcggct cctcgcggcg atcagggagg
    21301 aactgtaggc gttcgcgccc tcatcctggg cggcgcccgc agcgggaaga gcgctctcgc
    21361 cgagcggctg gcatcctcaa tgggaggcgg gcgtgtcctc tacgtggcaa ccgccatcgt
    21421 ggacggggac gaggacctgc agcggcgcat aagactccac agggagcgaa ggcctcatgg
    21481 gtggggaacg ctggagatcg gtgacctgga cgaagtactg gaagcggcgg atgaatggga
    21541 tgccatcctg ctcgactccc ttaccctgtg ggtctcggcg aggggtgagc cggcgctccc
    21601 cgagttcgat cggttcctga ctcgtgcagg agaactcggc gtgcctctct ttctggtgag
    21661 cgacgaggtc ggactcgggg tcgtgcctga gagcgaggcg ggtagaaggt tccgcgacct
    21721 gctcgggctg gtcaaccagc gggcggccga ggcggcggag gaggtccacc tctgcgtcgc
    21781 cggtatgagc atacgattga agtagatgaa ggcgctcctc tccttcttca cggtccttcc
    21841 ggttcgtggg gctactctgg aagaggccgc gcgggagatc catctgctgc ccttgatcgg
    21901 gctggttact ggattgccag gggccatcct gctattggct acccccgtcg tgcctcccgg
    21961 tgtagccgcc acgctcgcgc tgggggccgt gcttctcgcc gccggcctgc accacgccga
    22021 cggcgtactc gacgtcggcg acgctctgat ggtccacggc agccccgacc ggcgccgcga
    22081 agtgctccaa gacgcccgcg tcggcatcgg cggtctcggc gcgctcttcc tggtctacgc
    22141 acccaccgtg ggcgcactcg ccgcgttgtg cgccgtctct ccaggtcgtg ccgcgctcgc
    22201 cctgttcgcc gctgaggtcg cggtccggtc ggcgatgctc ctcttgttgg tcttcggtga
    22261 gcccgcagat ttgcgttcct cctccgtacc ctttgtgcga gcgcttaccg ggccacaccg
    22321 tactcccgcc gtcatcgttg cgttgcttgc gccgctgcca ttccttctgg cgatgagcgc
    22381 ccttctggcg ccgctcgcca tcctggccgt ccccctggtc gcgctcggtc tcctacggat
    22441 ctccgcggcg aagtttggtg ggatcggcgg cgatgtagtc ggggctacag gggaggtgtg
    22501 ccgcgccgtg gtgctgatcg cgctctccgc aatgatgtag ctcctgggag ccggtgcccg
    22561 gacgaagatt tcattcgcga cttacggcac cccgtggcaa gacccggtcg cgcgggcgaa
    22621 tcgcccgaac ggggtcttga caggcccacc cataacgcct agcctccagg ggtatgtccg
    22681 tcgagttcct gatcacgtcg ctagtggtgg tcctcatccc tggcaccggg gtcctctaca
    22741 cgctctccag ctccataggc ggcggccgca ggcgcgggct gttcgcggcc ataggctgca
    22801 ctttaggcat cgttcctcac ctgctggcgg ccatgctcgg cttatccggg atcatgcagg
    22861 ccgggtcggc cgtcttcgag gtggtcaggt gggctggggt ctgctacctc gtcttcgtgg
    22921 gcgcaacgat gatccgcgac gctggcaaga tatccctcga tgacgtagag gccggacctg
    22981 atcccatggg ccctgtcgta cggcgcggta tcctgttgaa cgtcctcaac ccgaagctga
    23041 ccctgttctt cttcgccttc ctcccgcagt tcctagacgc ttcgccgggc ctacacagac
    23101 tggagttgat ctggctgggc ggggtgttca tgctcatgac cctcgcagtg ttcggcgtgt
    23161 atgcgctggc gagcgctgcc tttcgcgacc tcgtgctcgg tgcgcccgtg gtccgacgct
    23221 ggatcgagcg gacgcttggt gcgttcctga tggccttcgc cgccaggctc gcccttacgg
    23281 accgatgagc ttcgaagaac tgcggttgcc cgctaacgcc aaccaccacc aagacgggag
    23341 attggtcgcc atgttcgcct acgatgaagc tgtcgccgaa cggtacccga cgatccgcgc
    23401 ctgaatcatc ttccacggct ccgagagggc atcgcgggac ccgggtctca caggacttga
    23461 ccggccggca tgtttttcga agagggttga gaacttcttt gcaaatcgct ctgagtcgcg
    23521 gttccaagtg ggcagttcgc gaaccgcccc tacagattcc ctcaagacgc cagatcgtca
    23581 ccgggtttca ttacgtggac cttgaggtcg gggacgaggg cggaggtgtg ctcctggaac
    23641 tcctccgggg tgcctggcaa gaacgggaaa gtcccgtagt gcattgggac tacgtgctct
    23701 acgccgagga gcctggccgc gtgggccgcc tcgagtgggc tcattactac ctggttgccg
    23761 atgggcagca gcgcgaggtc ggggttgtag atctcgccga tcaggcgcat gtcgccgaag
    23821 accgcggtgt cgcccgcatg gtagagcttg aaaccgctct cgaactcgat gacgtagccc
    23881 atcggctcgc cgccgtagat gtagctgccg tcgtcgagct gtatgcttga agagtggaag
    23941 gcgttggtcg cggtcacctt gaggccgccg acctcgaccg tgccgccctt ctggatcggc
    24001 atggcgttct cgatcccctg ccgctgcagg taggagaaga tctcgaagtt ggagaccgtc
    24061 gctgaccccg tctgcccggc gagaggctct acatccgaga agtggtcgaa gtggccgtgg
    24121 gttatgagga tcgtgtccag atgaccgacc tgcttcatgt cgtccggggt ctgcgggttc
    24181 tcagagagga aggggtctat gatgatctgt tcgccgcccg ccgtcacgaa cctgaacgtc
    24241 gcgtgcccca ggaaggtgat tctcgctccg cctagcatct gttccacgat actcctccgt
    24301 acgttgacca aacacgatca agaaaagaac gtccagatgt atacccaccc gacaaaccag
    24361 agaaacagcc gccacctgcg tataatcctg cgggcgtccc taccggggct ctgcgtcgag
    24421 accacgaacc tcagggggtg ccaatgaaat ctacccttct cctcgccgta ctagcgggcg
    24481 tgtgcgtggc cgtccagacc agcctcacag gagcggccca gcgaacgctc gggcccctgg
    24541 tcctggtcgc gatctcgggt cttacgacca gcatgttcgc gctctcgctc tcgttcatgg
    24601 tggcgaaacc ggagtttacg ggccgcgcag tagtttacgc cgcggcctcg ggcgtcctcg
    24661 gggcggtgat cttgggctct atcgccgtcg cagccgggca ggccggggtg gcgcgcgccc
    24721 tctcgctcgt tatagccacc cagctgcttg tgggcctcgt cctcgacgcc ttcggggtct
    24781 tcggtgccgg ggccgatttc agcgtcctga aggcgctggg agtactcttg atcatcgccg
    24841 gcggcgtatt ggtggtcagc tattagagcc agtcagcacg ctacgcccct cgggctccgc
    24901 ttgtcagccg gtcagccagg actcttgcgc aagcgaaagc tgaagtgctg agaagcgaag
    24961 gcaaagcctg agcgtgctga aatgcacggc gctgtaagat acgggtaggg acatcgacgc
    25021 tgagaaggga ggctggagtg gggtatctgc ccatcgagga ccac
//
LOCUS       DATMVF010000271         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_500163, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000271 DATMVF010000000
VERSION     DATMVF010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500163"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VN178_14150"
     CDS             <1..441
                     /locus_tag="VN178_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82157.1"
                     /translation="GVPLAATSTEVEIAVEAPSGPAFAAATESQRPRKVYLKVENVKG
                     EDLAASSYLVHLGLPPGASAAEYEDRRVGKVSMFGVLEASQSDEEHSGSGLTFSFDIT
                     GIFERLQEAEEFDPEHLRVTFTPVHASEEQSGNVRAGRVSLFYA"
     gene            452..1147
                     /locus_tag="VN178_14155"
     CDS             452..1147
                     /locus_tag="VN178_14155"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HWS82158.1"
                     /translation="MSATTQAAYGVVPLRLRRIGWRHPEWWVILIAAAAWALILRHAA
                     HSHTVPSASQTIIATAVMVVAMMLPLTLANVRHVALSSLWRRRHRAMAAFLVGYLCCW
                     AVVQTAIQGTLGFLAPLLGWTLVAGLLMVTAVLWEISPGRKRRLRRCRLTVPLAPRGW
                     RADNACVRYGVITGFGCVTTCWALMAAVAAFSHSVLVMVVVFVVQISDRYQRRPSPVL
                     TSLAILGVCLLSF"
     gene            complement(1241..1888)
                     /locus_tag="VN178_14160"
     CDS             complement(1241..1888)
                     /locus_tag="VN178_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWS82159.1"
                     /translation="MYRALLFDLDGTIAETDSLHLPTWVDALQPHGVEVDEEFYRDRI
                     SGRSTAEIVRELLPDLTDEQGRSIGDAKEASFRERASELEPLPGLVDFVERGRKRGMR
                     IALVTNAPEENVEAILPALKLRGLFDVVVLADEVEAVKPDPAPYRAALKRTGVPAGRA
                     LAFEDSVSGITSSVAAGIPTVGIASSQDPKRLLGAGAFTAAEDFTDPQLRALIDA"
     gene            complement(1893..2231)
                     /locus_tag="VN178_14165"
     CDS             complement(1893..2231)
                     /locus_tag="VN178_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82160.1"
                     /translation="MSSVYTILTDWLAWERRGTANRVLFVLSLSLAAFVTLTFAGASL
                     DPDPGNFARYFAFYWVWGLCAIGVLLGMAEYKTSPLRKGKERGHLFILLYIGALLIVT
                     RALNMVLYAG"
     gene            2315..>2754
                     /locus_tag="VN178_14170"
     CDS             2315..>2754
                     /locus_tag="VN178_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS82161.1"
                     /translation="MRRLLALSCAIVLVDTIFYAALYPLVPYFQEEFGLSKSVVGILS
                     GAYGAGVLIGSVPGGFLASRSGIRITALVGLILVSVTSVMFALVDTTWLLVAVRFAGG
                     FGSALSWVAAFTWLVDRAPEERRAEMIGVMISAAVVGALLGPVLG"
BASE COUNT          448 a          862 c          908 g          536 t
ORIGIN      
        1 ggggtgccgc tcgcagccac atcaaccgag gtcgagatcg ctgtcgaggc tccctctgga
       61 cccgcgttcg ccgcagcgac cgaaagccag cggccccgca aggtgtacct gaaggtcgag
      121 aacgtgaagg gagaggatct cgcggcctcc agctacctgg ttcatttggg tctgccccca
      181 ggggcgagcg ccgccgagta cgaggatcgg cgcgtcggca aggtctcgat gttcggcgtg
      241 ctcgaagcct cccaaagcga cgaggagcac agcgggagcg ggctgacctt ctccttcgac
      301 atcacgggca tcttcgagcg tttgcaagag gcggaggagt tcgacccgga gcacctgcgc
      361 gtaaccttca ctcccgtgca cgccagcgag gagcagtccg gaaacgtaag agcgggtcgc
      421 gtcagcctat tctacgcctg agctctctgg catgtctgcc acgactcaag ccgcatacgg
      481 agtcgtccca ctaaggctgc gccggatcgg atggcgccac ccagagtggt gggtcatctt
      541 gatcgcggcg gcggcctggg cgctcattct tcggcacgcg gcccactccc acaccgtccc
      601 aagcgcaagc caaaccatca tcgcaaccgc cgtgatggtg gtggcgatga tgctgcctct
      661 caccctcgcc aacgttcgcc acgtggccct ctccagcctg tggcgccgca ggcacagggc
      721 gatggctgcc ttcctggtcg gctacctctg ctgctgggca gtggtccaga ccgctatcca
      781 gggaacttta ggatttctcg ccccgttgct agggtggacg ctcgtggcgg gtctcctcat
      841 ggttacggcg gtcttgtggg agatctctcc tggcaggaaa cggaggctca ggcgctgccg
      901 actaaccgtg ccgctcgccc ctcgggggtg gcgagcggac aacgcgtgcg tccggtacgg
      961 cgtgatcacc ggcttcggtt gcgtaacgac gtgctgggcc ctgatggccg cggtcgcggc
     1021 cttctctcac agcgtcctgg tcatggttgt cgtgttcgtc gtgcaaatca gtgaccgtta
     1081 tcagcgacgt ccgtcccccg tattgacatc gctcgccata ctcggggtgt gcctgctgtc
     1141 attctgaaga aggctggaag tcggtccggt atccggctcc aagttcgagc tcgtgatggg
     1201 catggactca acgatgactc caccatggct aggggctgtc ctacgcgtcg atcagggcgc
     1261 ggagctgcgg gtcggtaaag tcctctgccg ccgtgaaggc ccctgctccg aggagtcttt
     1321 tggggtcctg gctcgaggcg atgcctaccg tcggaatgcc cgcggcgacc gaggaggtga
     1381 tacccgagac cgagtcctcg aaggccagcg ctcttccggc agggacgccg gttctcttta
     1441 gggcggctct gtagggtgct ggatccggct ttacagcctc gacttcgtcg gcgagcacga
     1501 ccacgtcgaa gaggcctcga agcttcagtg cgggcagtat cgcttcgacg ttctcctcgg
     1561 gggcgttggt gacgagggcg atccgcatcc ctcgcttcct gccccgctct acaaagtcca
     1621 cgagacccgg cagaggttcc agctcggagg cgcgctcgcg gaagctcgcc tccttggcgt
     1681 caccgatgga tcttccttgc tcgtcggtga ggtcggggag cagttcgcgt acgatctcgg
     1741 ccgtgctccg cccgctgatc ctgtcgcgat agaactcctc gtcgacctcg acgccgtgtg
     1801 gttggagcgc atccacccac gtcggcaggt gcagggagtc ggtctccgcg atcgtaccgt
     1861 cgaggtcgaa gagcagggcc cggtacaaac cgctaaccgg cgtagagaac catgttcagg
     1921 gcgcgggtca cgatgagcag cgcgccgatg tacaggagta tgaagaggtg gccgcgttct
     1981 ttgcccttgc gcaagggact cgtcttgtac tcggccatcc ccagaagcac gccgatggcg
     2041 cagagacccc acacccagta gaacgcgaag tagcgcgcga agttgccggg gtcggggtct
     2101 agcgaagccc ccgcgaaggt tagcgtcacg aaagcggcca gcgagagcga caggacgaac
     2161 agcaccctgt tcgccgtccc cctgcgctcc cacgcgagcc agtcggtcaa gatggtatag
     2221 acactgctca cgctggttat gttagcagcc agagaacagg ccgccgtgaa agcttccggc
     2281 tcacgcgccg tactcaggca ttagaatgac cttcgtgcgc cgactccttg ccctcagctg
     2341 cgcgatagtg cttgtggata cgatcttcta cgctgcgctc tacccgctcg tcccttattt
     2401 ccaggaggag ttcggcctct ccaagtccgt ggttggcatc ttgagcggcg cctacggggc
     2461 gggtgtcctg atagggagtg tgcctggtgg attcctcgct tcccgctcgg gaataaggat
     2521 caccgccctc gtcggattga tcctcgtgtc ggtaaccagc gtgatgttcg cgcttgtgga
     2581 cacgacgtgg ttactcgttg cggtgcggtt cgcaggcggg ttcggtagcg cgctctcatg
     2641 ggtcgcggcg ttcacctggc tggtggaccg ggctccggag gagcggcggg ctgagatgat
     2701 cggggtgatg atcagcgcgg ccgtggtcgg ggcgctgcta ggacccgtgc ttgg
//
LOCUS       DATMVF010000272         6752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_544540, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000272 DATMVF010000000
VERSION     DATMVF010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544540"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VN178_14175"
     CDS             complement(<1..434)
                     /locus_tag="VN178_14175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS82162.1"
                     /translation="MSEAGGITRRKTFALVLLCCASFVAVLDLTIVAIALPSVRRELG
                     FGGGDAQWILTGYALSFGGLLLFMGRAGDLYGRRRLFVGGLALFAAASFLGGLAWAPW
                     VLVLARLLQGVGGAALVPASLALVSATFAEGEERNRAMGVYGA"
     gene            557..1156
                     /locus_tag="VN178_14180"
     CDS             557..1156
                     /locus_tag="VN178_14180"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWS82163.1"
                     /translation="MDQRIPEGEVPEEVRLVNEFLNTLHLERFGEHAAKPEEEREGLR
                     SPEMFKAWLVGRGLLGREEAVSEADRRLAVELRDTLRSAASANSGPSRERSEKVGRTF
                     GEFPLVVRVGVGSGPELVSGEGGAKGALGTILAGVVVASSKGTWARMKICAAEDCRWA
                     YYDHSKSRTGRWCAMETCGNRHKTRRYRRKKRASALQGG"
     gene            1194..1517
                     /gene="mihF"
                     /locus_tag="VN178_14185"
     CDS             1194..1517
                     /gene="mihF"
                     /locus_tag="VN178_14185"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HWS82164.1"
                     /translation="MLRRAPERTADERRDALEEANRVRFARAATKRDLKSGRLGIYDL
                     LMDPSEELRGAKVEEMLLAVKGMGRIKVTRVMRDAGISRSKTLVGLTHGQRDRLIRAL
                     GCEED"
     gene            1502..2092
                     /gene="gmk"
                     /locus_tag="VN178_14190"
     CDS             1502..2092
                     /gene="gmk"
                     /locus_tag="VN178_14190"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWS82165.1"
                     /translation="MRGRLIVVSGPSGAGKSTLIKASLEEVPELAYSVSATTRGPRPG
                     EVDGKDYIFLSRREFERWIQEDRFLEWAEYSGNLYGTPERKVEEFLDEGKSVILELEL
                     QGARQVQLKRPEAVMVFVRAPSLEETRRRLTGRATETEEALESRLATAVGEVAARDEF
                     DHEVVNENRERARRDMIEKMEQIVRGGKDVARPEDR"
     gene            2067..2429
                     /gene="rpoZ"
                     /locus_tag="VN178_14195"
     CDS             2067..2429
                     /gene="rpoZ"
                     /locus_tag="VN178_14195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWS82166.1"
                     /translation="MLHDLKIDELLDKVDSRYGLAVASAKRARQINEYTSTLGLNDAL
                     GIPGPQVHTRSQHPLTIAIEELRQDKLEVAFRQPAEDTDEEIGAADADDFISPPAGAG
                     VPQDMDIDSGAPGDEGAA"
     gene            2454..3524
                     /locus_tag="VN178_14200"
     CDS             2454..3524
                     /locus_tag="VN178_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HWS82167.1"
                     /translation="MILLRVGASPGALAIPDVAAEIAGTDNRVEVILDQRARRFVGPA
                     AFRDVALVGDPSETPEAVLFAPAPADTLARLARGMVTRFSPSAVVGPDLDAGTAGHPA
                     VRENLDLLREDGYRIVEGREGRMASTGEIVAGLLGSLGGPMDGLRLLVTAGGTREPID
                     SVRFIGNRSSGKMGLAVAREALRLGAEVSVVAANVDTIEPGTRYRAVETVEELKEAVL
                     EQAADADALVMAAAVSDFTPASRVSEKIRRSSGTTSLELAPTADILEAVRERYPDLFV
                     VGFAATHGDPVADAREKLAKKGADLVVGNDISKEGVGFGAAENEVYVVGREEERFVPR
                     ASKAQIARVILDSMIAEMDEER"
     gene            3527..4762
                     /gene="metK"
                     /locus_tag="VN178_14205"
     CDS             3527..4762
                     /gene="metK"
                     /locus_tag="VN178_14205"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWS82168.1"
                     /translation="MVERAAGPTTWNAADDQKLKASHLFTSESVTEGHPDKIADQISD
                     TILDAAIADDPMSRVACETLVTTGLVVVAGEITTSTYVDISTLVREMVSKIGYTRAKF
                     GFDAETCGVIVSIDPQSADIALGVDRSLEARESEGLDEIGAGDQGMMFGYACEETDEL
                     MPLPITLAHALTRRLSDVRKSRVLDYLRPDGKSQVTVRYEGDRPVGIEKILISAQHRD
                     GVEAHIRDDLLETVIRPVMPEELFDPRATEIMVNPTGRFVTGGPQGDTGLTGRKIIVD
                     TYGGSAPHGGGAFSGKDPTKVDRSASYAARWVAKNLVAAGLAGRCLVQVAYAIGVAHP
                     VSVMVETFGTAKTDPATLGTLVQEHFDLRPGAIIRDLNLRNPIFARTAAYGHFGRREP
                     GFTWEDTSLSDDLRRAAGF"
     gene            5548..6126
                     /locus_tag="VN178_14210"
     CDS             5548..6126
                     /locus_tag="VN178_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWS82169.1"
                     /translation="MTRSTFLLGVGGAAALASIRLQDSEAARAGPRNVLWVITDDQPQ
                     YMMESLTVTTANIRDLGIEFTLGSADVPLCGPARVSLLTGLSVTTHKCDTNRTWSKFL
                     NSTLGLQERTVARYVKDVGYATGHFGKYVNGYGNDGTVPPHWDRWRATKGDASDAGGN
                     VTTPNQGNVDGTWIDIIGPLCVGGRAVCPVRA"
     gene            6119..>6752
                     /locus_tag="VN178_14215"
     CDS             6119..>6752
                     /locus_tag="VN178_14215"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWS82170.1"
                     /translation="MRERVESPWFAQYCPSIPHAPYFPTPDSEHLYDGAIRNVPSVNE
                     ADMSDKPTWMRNLPLADTAAVQAEYEGKLEELADLDSKGMRPILAALEATGQLANTVI
                     FFTSDNGYLHGEHRLRKKDRPYWESSQVPFFVKGPGVKGPGVSSRTIRTVLVNHTDLM
                     PTTCEIAGVPVASLEVDGRSMLPYLATGSFSGWRKRMLVTGSNDVGGQLNP"
BASE COUNT         1352 a         2093 c         2200 g         1107 t
ORIGIN      
        1 gccccgtaga cgcccatggc acgattccgc tcctcgccct cggcgaaggt ggccgacacc
       61 agcgcgagcg aagccggcac gagcgccgcc ccgccaaccc cctgcaatag gcgcgccaac
      121 accagcaccc agggtgccca cgccagaccg cccaggaacg aggcggcggc gaacaaggcg
      181 agcccgccca cgaacaggcg ccgccggccg taaaggtcgc cggcacggcc catgaagagc
      241 aggagacccc caaaagagag cgcgtatccg gtgaggatcc actgggcgtc gcccccaccg
      301 aaacctagct ccctccgcac cgaaggcagc gctatagcga cgatcgtgag gtccagcacc
      361 gccacaaacg aggcgcaaca caacagcacc agcgcaaatg tctttctccg cgttatcccg
      421 ccggcttcgg acaccgtgcc ttcctccaaa gctatcgcat cattaacaac accattaata
      481 cattataccg gtgtcgaact cactctaaac cgcctatcgc gtacaatggt gtcgagcagt
      541 actgaggagg attcgggtgg accaacgcat accggagggc gaggtacccg aagaggtcag
      601 gctcgtcaac gagttcctca acacgctcca tttggagagg tttggggagc acgccgcgaa
      661 accggaggag gagagggaag ggttgaggag ccccgagatg ttcaaggcct ggctcgtcgg
      721 gcgtgggctt ctcgggcggg aagaagcggt gagcgaggcg gatcgacgac tggcagtaga
      781 gctgcgagac acattgcgtt cggcggcctc cgccaactcg ggcccgtccc gagagaggtc
      841 ggagaaggtt ggccgaacct ttggggagtt cccgctcgtg gtccgtgttg gtgtaggttc
      901 ggggcccgaa ctcgtgagcg gggagggcgg ggccaagggt gctttgggca cgatcctcgc
      961 cggcgttgtc gttgcgtcct ccaagggcac gtgggcgcgg atgaagatct gcgccgccga
     1021 ggactgtcgg tgggcgtact acgaccactc caagagtcgc acgggcaggt ggtgtgccat
     1081 ggagacttgc ggcaaccgcc acaagaccag gaggtaccgt cgaaagaagc gcgctagcgc
     1141 gctgcaggga ggttagaccg ggtgccttcc ggcgatggta tactaacccg ttgatgttga
     1201 ggcgggcacc agaacggacg gctgacgagc gtagggacgc gctcgaggag gccaacaggg
     1261 tgcgcttcgc cagggctgca accaagcgtg acttgaagag cggacggttg ggcatctacg
     1321 atttgcttat ggacccttcc gaggagttga ggggcgccaa ggtcgaggag atgcttctcg
     1381 ctgtcaaagg tatgggccgc atcaaggtca cacgcgttat gagggacgct ggcataagcc
     1441 gctccaagac cctggtgggg ctaacgcacg ggcagaggga ccgtttgatc agggctctag
     1501 ggtgcgagga agactgatcg tcgtctccgg accctccgga gcaggaaagt cgaccctgat
     1561 aaaggcttcc ctggaggagg tgcccgagct ggcctactcc gtctcggcca cgacccgggg
     1621 gccgcgtccc ggcgaggtag atggcaaaga ttacatcttc ctctcgcgcc gggagttcga
     1681 gcggtggatc caggaggacc gctttctgga gtgggccgaa tactctggta atctctacgg
     1741 gaccccggag cgcaaggtcg aggagttcct ggacgaggga aagtccgtga tcctcgagtt
     1801 ggagctccag ggtgcgaggc aggtccagct aaagcgtcct gaggccgtga tggtcttcgt
     1861 ccgagccccg tcgctggagg agacccgccg aaggctcacc ggaagggcca ccgagacaga
     1921 agaagcgctg gagagccgtc tggcgaccgc cgtaggcgaa gtcgcggcca gggacgagtt
     1981 cgaccacgag gtcgtaaacg agaatcgcga gcgggcccgc agggacatga tcgagaagat
     2041 ggagcagata gtaagaggag gcaaagatgt tgcacgacct gaagatagat gagcttctgg
     2101 acaaggtgga ctcccgttac ggcctcgccg tagccagcgc caagagggcg cgccagatca
     2161 acgagtacac ctcgaccctg ggcctcaacg atgccctcgg catccctggc ccgcaggtac
     2221 acacccggtc ccagcatccg ctgaccatcg ccatagagga gttgcgccag gacaagctgg
     2281 aggtcgcctt caggcagccc gcagaggaca cggacgaaga gatcggggcg gccgatgcag
     2341 atgacttcat cagcccgccc gccggcgccg gcgttcccca ggatatggac atcgactcgg
     2401 gcgcaccggg cgacgaaggc gctgcctgag acaacgacgt aagccttgac accttgatcc
     2461 ttctccgtgt cggagcctct ccgggtgccc tggcgatccc cgatgtggcc gcggagatag
     2521 ccgggacaga taacagggtg gaggtgatcc tcgaccagag agcccgtcgc ttcgttggtc
     2581 ccgcagcgtt ccgtgatgtg gccctggtcg gtgatccctc ggagacgccc gaggccgtgc
     2641 tcttcgcccc ggcacccgcg gatactctgg ccagactcgc ccgcggcatg gtcactcgct
     2701 tctcgccaag cgctgtcgtg ggcccggatc tcgacgctgg cacggcgggc caccccgccg
     2761 tacgagagaa cctggatctt ctccgagagg atggctaccg catcgtcgaa ggccgcgaag
     2821 gcaggatggc ttccacggga gagatcgtcg ccgggttact cggcagtctc ggaggcccga
     2881 tggacgggct gcggctgctc gtgaccgctg gaggtaccag ggagcccata gacagtgtgc
     2941 gttttatcgg gaaccgctcc tcggggaaga tggggctcgc ggtggcccgc gaagctttgc
     3001 ggctcggcgc cgaggttagc gtggtggccg ccaacgtaga tacgatcgag ccggggacca
     3061 gataccgcgc ggtagagacc gtggaggagt tgaaagaggc ggttctggag caggccgctg
     3121 acgcggacgc gctcgtcatg gccgctgcgg tctcggactt cacgccggcc tcgcgagtaa
     3181 gtgagaagat ccggcgtagc tcggggacga caagcctgga gctcgccccc acggccgaca
     3241 tactcgaggc cgttagggag cggtatcccg acctctttgt ggtaggattc gcggcgactc
     3301 acggagaccc cgtggccgac gcccgcgaga agctcgcaaa gaagggagcg gacctcgtgg
     3361 tgggcaacga catctccaaa gaaggcgtag gattcggcgc cgccgagaac gaggtctacg
     3421 tcgtcggccg cgaggaggaa cgattcgtcc ccagggcctc caaagcacag atcgcccgtg
     3481 tcatcctgga cagtatgatc gcggagatgg acgaggagag atagagatgg tggagagagc
     3541 ggccggcccg accacctgga acgcggcgga cgaccagaaa ctgaaggcat cccacctctt
     3601 tacctcggag tccgtcaccg agggccaccc cgacaagata gcggaccaga tctcggacac
     3661 catcctggac gcggcgatcg cggacgaccc catgagcagg gtcgcctgcg agacgctggt
     3721 gacgacgggt ctcgtcgtcg tggccggtga gataacgacc tcgacctacg tggacatctc
     3781 gacgctggtg cgcgagatgg tttccaagat cggctacact agggccaagt tcggcttcga
     3841 cgccgagacc tgcggcgtga tcgtctccat agacccgcag tccgcagaca tcgcactcgg
     3901 ggtagataga tccctcgaag cgagggagag cgagggtctc gacgagatag gggccggcga
     3961 ccaggggatg atgttcggct acgcctgcga ggagacagat gagttgatgc cgctcccgat
     4021 cacactcgca cacgctctga cgcgccgtct ctccgacgtc cgcaagagcc gcgtcctcga
     4081 ctacctgagg ccggacggga agagccaggt tacagtccgc tacgaggggg acaggccagt
     4141 cggcatcgag aagatcctga tctcggccca gcaccgcgac ggggtggagg cccacataag
     4201 ggacgatctc ctggagacgg tcatacgccc ggtaatgcca gaggaactat tcgacccccg
     4261 cgcgaccgag atcatggtca accccacagg ccggttcgtt acgggagggc ctcagggcga
     4321 cacgggcctc acggggcgca agatcatcgt ggatacctac gggggatcgg ccccgcacgg
     4381 cgggggcgca ttctctggca aggacccgac caaagtcgac cgctcggcct cctacgccgc
     4441 caggtgggtg gcgaagaacc tcgtcgcggc gggactcgca ggccggtgcc tggtccaggt
     4501 ggcttacgcc ataggcgtcg cccatcccgt gagcgtgatg gtcgagacct tcggcaccgc
     4561 aaagaccgac cccgccaccc tcgggacgct ggtgcaggaa catttcgacc tcaggcctgg
     4621 cgccatcatc cgcgacctga acttgcggaa ccccatcttc gcgcggaccg ccgcatacgg
     4681 ccacttcgga cgcagggagc cagggttcac ctgggaagac acctcgctct cagatgacct
     4741 ccgccgcgcc gccggcttct gatgtcttac ggagttctga gaaggtcagg ttcgggccgc
     4801 cgagcgtcgt aacgtgagga gggtgatatc gtcctcattc tcccaccccc gctcgcacca
     4861 cgcacccagg attcgccctt gccgagctga tcatagccat taatccgatc gtcggccgcc
     4921 agtcctttct gttgtaaccg ctcggttctc ctcgagcacg ccgcttacac ccttgatcag
     4981 tgctttgaac tgctgcatcc tttcctcacc aagccgttgg acgcattcgt gatccaagct
     5041 cgtcatgatc tcatctccta cccgcctgac ctcccatcct cgccgcgtca agcggataat
     5101 cttggccctc ccgtcggtag ggtccggaac tcgctctacg taaccggctg cctcgagctg
     5161 gttgatggtg tagagcaagg cctgcttggt aatgtgcgtc cgaggaggct tcgatgggag
     5221 cgagcccgca caggacctgg cgaaggaggc cccgctaccc ggagcctccg ggggttgtct
     5281 ctaaggccaa gttcaactgc atcgcggcgt tggccacgtc ggcttgacaa acagtgcctt
     5341 ctcatagatg tttactgaca tttccactct cattctccga cggttgccga gaattcatcg
     5401 agaaatcatc gctgcttgct agcgtgggga cgttgtgcaa cttggcggac cttatgctgc
     5461 tcgctttaag cgtcttgcgc agcctgccca aagatgctag tgatcggcca ggatagagaa
     5521 cgggagggag agtcaatgaa gcgaaagatg acgaggagca cgttcctttt gggggtgggt
     5581 ggggcggcag ccctggcctc tatcaggctc caagacagcg aagccgcgcg agcggggccc
     5641 cgaaacgtcc tctgggtgat aactgacgac cagccgcaat acatgatgga atctctgact
     5701 gtgaccacag ctaatatcag ggatctaggg atcgaattta cgcttggctc cgcggacgta
     5761 ccgctgtgcg ggccggcgag ggtgagccta ctcacgggcc tcagcgtgac cacccacaaa
     5821 tgcgacacga acaggacctg gtcgaaattc ctcaacagca cgcttgggct gcaggagcgc
     5881 actgtcgccc gctacgtgaa ggacgtaggc tacgccaccg ggcacttcgg caagtacgta
     5941 aacggctatg ggaacgacgg tacggtgccc ccccactggg accgttggcg cgccacgaaa
     6001 ggtgacgcct ccgacgcggg cgggaacgta acgactccca accagggcaa tgtggacggc
     6061 acgtggatag acatcatcgg ccccctctgt gtgggcggcc gagcagtgtg cccagttcgt
     6121 gcgtgagcgc gtggaatccc cttggttcgc ccaatactgc ccgtccatac cgcatgcccc
     6181 ctacttcccg accccggact ccgagcatct ctacgacggg gcgattcgga acgtgccatc
     6241 cgtaaacgag gccgacatgt ccgacaaacc gacgtggatg cgcaacctcc cgctcgccga
     6301 caccgctgcc gtccaagccg aatacgaggg gaaactggag gagcttgccg acctcgattc
     6361 gaaggggatg cgtccgatcc tggcggcact ggaggctacg ggccagctcg ccaacacggt
     6421 catcttcttt acctcagaca acggctacct ccacggcgag catcgcctgc gcaagaaaga
     6481 ccggccatac tgggagagtt cccaggtacc cttcttcgtg aaaggaccgg gtgtcaaagg
     6541 accgggtgtc agtagccgca cgatcaggac cgtgctcgta aaccacacgg atttgatgcc
     6601 cacgacttgc gagatcgccg gagtgcccgt cgcctcgctc gaggtggacg gccgctccat
     6661 gctgccgtac ctcgccaccg gctccttctc cggttggcgc aagaggatgc tcgtcacggg
     6721 ctctaatgac gtgggagggc agctgaaccc cg
//
LOCUS       DATMVF010000273         4898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_502553, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000273 DATMVF010000000
VERSION     DATMVF010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502553"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VN178_14220"
     CDS             <1..754
                     /locus_tag="VN178_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWS82171.1"
                     /translation="AGGASIERDPRAAQAAVDGFVIYSMSDEESLLAAALQRRLPAVV
                     VDQPRREGVPFVGVDDEGAARTAAEHLLDLGHERFAIVSFGLSPDGHQGIADSDRQER
                     TTFRVSRMRLQGYRAALEAAGLSWSGVPVYECQGSTRGLGYLAVEALFRSHQEPTAIL
                     ALSDQLALGAIQWMTERRISVPQDVSVVGFDDIPAAASADPPLTSVHQDHAEKGRLAG
                     RMLVAQLREEAAPSAELLATRLVVRGSTALNP"
     gene            complement(831..1967)
                     /locus_tag="VN178_14225"
     CDS             complement(831..1967)
                     /locus_tag="VN178_14225"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HWS82172.1"
                     /translation="MALLVGSACSAPDERRGGDVIAGASKQRTVDLLPRQTETDGRIG
                     DSVEAGNLRLRIFDVRADDRVYAISGPGMRPVTRGNTSGEYVAVDYLVKNISGSPMTT
                     GAKATLLDDQGNSYRQDRSIGPPSGGTDGMELGTDQTKASTMFFEVPNGIIPTTLEIE
                     TLSGRKARFDLLARDMEGVPPEDYLRVYHLYFNEQAYEEAYEMFDPASVQNITLGEWL
                     SFYEPLWGKRYVSLDDLRSLSEGSGRAVFQMTRTFYDADGDIVANPEVNPSVIQEMVE
                     VDGEWKLVMRDDLVFDIIAVIGPDETPEPEATEPEKTAPERTQPPSTVLETTEQTEAT
                     GSAPPRDYECEDFESQEEAQLYLAPGDPYNLDEDGNGVACETLP"
     gene            complement(2048..2800)
                     /locus_tag="VN178_14230"
     CDS             complement(2048..2800)
                     /locus_tag="VN178_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWS82173.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGALFGKLSKAITVAARDGGGDPE
                     MNAALGLAVQKAKDANMPNDNIQRAIDKGTGAGSDADTYERITYEGYAPGGVAVLVDV
                     LTDNRNRAASDVRYLFSKNGGKLGTSGSVAYLFERKGIILVPKDSTDEDELMEVALEA
                     GAEDVEEQENDYRVVTTPEDFTAVRDALGESEIAYDNAEITMEPQNSIDLDAGTARQT
                     LRLIDALEENDDVQEVYANFDISEEVMAEVVS"
     gene            complement(2797..3417)
                     /gene="pdxT"
                     /locus_tag="VN178_14235"
     CDS             complement(2797..3417)
                     /gene="pdxT"
                     /locus_tag="VN178_14235"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWS82174.1"
                     /translation="MVGNGETNGARRGEEGPKKIGVLALQGDFREHAEILHGLGVEPV
                     EVRAVEDLEGLAGIIVPGGESTTIGKMMVSSGLLDGIRSYFYKGGPVWGTCAGMVLAA
                     SATTGPRQPLLGLMNALVERNGFGRQVHSFEKDLDVDGFDEPFTGVFIRAPFFEDVGP
                     GVEVLSRIGDRVVAARGENILVTAFHPELTDDVRFHEYFLREVCSI"
     gene            complement(3407..4303)
                     /gene="pdxS"
                     /locus_tag="VN178_14240"
     CDS             complement(3407..4303)
                     /gene="pdxS"
                     /locus_tag="VN178_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWS82175.1"
                     /translation="MAENGQLTGTFRVKSGMAQMLKGGVIMDVVDAEQARIAEEAGAV
                     AVMALERVPADIRADGGVARMSDPEKIVEIQESVTIPVMAKARIGHFVEAQILEALEV
                     DYIDESEVLTPADEKHHIDKAVFEVPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGN
                     VVEAVRHMRAIVGGIRRLGALDHEEMMTEAKNLGAPYELVRWVAENGRLPVVLFTAGG
                     IATPADAALMMQLGADGVFVGSGIFKSGDPALRARAIVKATTHFKDAKAVAEASRGLG
                     VAMVGREMGDLAEGERLATRGW"
     gene            complement(4410..4604)
                     /locus_tag="VN178_14245"
     CDS             complement(4410..4604)
                     /locus_tag="VN178_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82176.1"
                     /translation="MGRLRRLARPREDGTAARVVQKVGEFARQEFTVALPAAKRAASH
                     AAQAFFKKFSEEYAKERKKN"
     gene            complement(4604..4807)
                     /locus_tag="VN178_14250"
     CDS             complement(4604..4807)
                     /locus_tag="VN178_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82177.1"
                     /translation="MSEERRRKGALLGVIAYVLVPGERRVQAAAHFRKAGFETLKGVG
                     ELVRPERQHDSSQTVNRERIEIE"
BASE COUNT          783 a         1687 c         1455 g          973 t
ORIGIN      
        1 agccggagga gcgtcaatag agcgcgaccc cagggcggca caggccgcgg tggacgggtt
       61 cgtgatctac tcgatgagcg acgaagaatc tttgctggcg gcggctttac agaggcgatt
      121 accggccgtg gttgtggatc agccacgtag ggagggagtg cccttcgttg gcgtcgacga
      181 cgaaggggcg gcccgaactg cggccgaaca tctcctcgac ctcgggcacg aaaggttcgc
      241 catcgtgtcg ttcggcctct caccggatgg ccaccaaggg atcgcggact ccgaccgcca
      301 ggagcggacc acgttccgcg ttagccggat gaggctacag ggctaccggg ccgcgttgga
      361 agcggccggt ctgtcctggt ctggggtgcc cgtctacgag tgtcagggca gtaccagggg
      421 actcggatac ctggccgtcg aggcattgtt ccgttcgcat caggagccga cggccatcct
      481 cgccttgagc gaccagcttg cgcttggcgc catacaatgg atgaccgagc ggcggatctc
      541 cgtgccccaa gacgtctccg ttgtcggatt cgacgacatc cccgcggcgg cgagcgcgga
      601 tcctcccctc acctccgtcc accaggatca tgccgagaag gggaggctgg ccggacggat
      661 gctggtagcg cagcttcgcg aagaagccgc cccaagcgcc gaattgctgg caacccgcct
      721 ggtggtccgg ggttcgacag ctctaaaccc gtgagcgctg actctccctt catcggctcc
      781 cacgcagtcc caaggtacgt tatcccgtgg ccggggaccc tcacaatcca ctacggcaag
      841 gtctcacaag ccacgccgtt gccgtcctcg tcgaggttgt aggggtctcc tggcgcgagg
      901 tagagctggg cttcttcctg gctctcgaaa tcttcgcact cgtagtcgcg tgggggcgcg
      961 gacccggttg cctctgtctg ctccgtggtc tccagaacgg tcgaaggtgg ctgggtgcgt
     1021 tcgggagccg tcttctcggg ttcggtggct tccggctcgg gcgtctcgtc gggtcctatg
     1081 acggctatga tgtcgaagac cagatcgtcg cgcatcacga gcttccactc gccgtccacc
     1141 tcgaccattt cttgtatcac ggaagggttg acctccgggt tcgccacgat gtcgccgtcc
     1201 gcatcgtaga acgtacgcgt catctggaag accgcccgac cagagccctc cgaaagtgac
     1261 ctgaggtcgt cgaggctcac gtaccttttg ccccacagag gctcgtagaa tgacagccac
     1321 tcgccgagtg tgatgttctg gaccgaggca gggtcgaaca tctcgtaggc ttcctcgtag
     1381 gcctgttcgt tgaaatacag gtggtacacc cgcaggtaat cctcgggcgg cactccctcc
     1441 atatcccggg cgagcagatc gaagcgggcc tttcggccag aaagagtctc tatctcgagc
     1501 gtcgtgggga tgatgccgtt gggcacctcg aagaacatcg tggaggcctt tgtctggtcc
     1561 gttccgagct ccatgccgtc ggtgccaccg gaaggaggcc cgatagatct gtcctgcctg
     1621 taggaattac cctggtcgtc gaggagagta gccttcgccc ccgtggtcat tggcgaaccg
     1681 gagatgttct tgaccaagta gtctacggcc acgtactctc cggaggtgtt cccccgcgtt
     1741 acgggccgca ttccgggacc cgagatggcg tagacccggt catcggcccg cacatcgaag
     1801 atccgcaaac gcaggttccc ggcctccaca ctgtcgccga tccttccgtc ggtctccgtt
     1861 tgccttggca ggagatccac ggtgcgctgt ttcgaggcgc cggcgatcac atccccgccc
     1921 cgtcgctcgt caggggcaga gcaggccgaa cccaccaaca acgccacgag caaggctacc
     1981 aagaccttcg cgatgcaggg cccccgtccg acccggccag agagggtgcc ggcgtaaaca
     2041 gggcccccta cgaaactacc tccgccatga cttcctcaga gatatcgaag ttcgcgtaga
     2101 cctcctgcac gtcgtcgttc tcttcgaggg cgtcgatcag gcgcagcgtc tgcctcgcgg
     2161 tccccgcgtc gaggtcgatg ctgttctggg gctccatggt gatctccgcg ttgtcgtacg
     2221 cgatctcgga ctcacctaga gcatccctga cggccgtgaa gtcctccggg gtcgtgacga
     2281 ccctgtagtc gttctcctgt tcttcgacgt cttcggcccc ggcttcgagt gccacctcca
     2341 taagctcgtc ctcatccgtg gagtctttgg gaaccaggat gatgcccttc cgctcgaaga
     2401 ggtaggcgac cgagccgctc gtgccgagct tgccgccgtt cttggagaag aggtagcgca
     2461 cgtcggaggc ggccctgttg cggttgtcgg tgagcacgtc gaccaggacg gctaccccgc
     2521 ccggcgcgta gccctcgtac gtgatccgct cgtaggtatc ggcatcggag cccgcgccgg
     2581 tgcccttgtc gatggcgcgc tggatgttgt cgttgggcat gttggcgtcc ttcgccttct
     2641 gcaccgcgag gcccagggcc gcgttcatct cggggtcccc gccaccatcg cgcgccgcga
     2701 ccgtgatcgc cttcgacaac ttgccgaaga gcgctccgcg cttggcgtcg gcggcgcctt
     2761 tcttgcgctt gatcgtcgac catttactgt gaccgctcat atggaacaaa cctcccgcaa
     2821 aaagtactcg tggaacctga cgtcgtccgt aagctcggga tggaacgccg ttaccagaat
     2881 gttctctccc ctggcagcca cgacccgatc tccgatcctg gagagcacct cgacccctgg
     2941 ccccacgtct tcgaagaacg gcgcccgaat gaagaccccc gtgaacggct cgtcgaagcc
     3001 atctacgtcg aggtccttct cgaaggaatg gacctgccgc ccgaacccgt tgcgctcgac
     3061 gagcgcgttc atcaggccaa gcagcggctg gcgcgggccc gtcgtcgccg acgcggccag
     3121 gaccatcccg gcgcacgtgc cccacacggg accgcccttg tagaagtagc tccttatacc
     3181 gtcgaggagg cccgaggaga ccatcatctt ccctatcgtc gtcgactctc cgccggggac
     3241 gatgatgccg gcgagccctt caaggtcctc cacggcccgc acctcgacag gctcgacccc
     3301 gagcccgtgc aggatctcgg cgtgctccct gaagtctccc tgcagggcga gcaccccgat
     3361 cttcttcgga ccctcttccc cgcgccgcgc gccgttcgtc tcgccgttac caaccacggg
     3421 tcgcgagtcg ctccccttct gccaggtcgc ccatctccct gcctaccatc gccacgccga
     3481 gacccctgct cgcttcggcc acggccttcg catccttgaa gtgggtcgtc gccttcacga
     3541 tggcccgcgc cctgagggca ggatccccac tcttgaagat cccgctgccg acgaacacac
     3601 cgtcggcccc gagctgcatc atcaatgccg cgtccgccgg agtggctatg ccgccggccg
     3661 tgaacagcac tacggggagc cgtccgttct cggcgaccca ccgcacgagc tcgtaaggtg
     3721 ccccgaggtt cttggcctcc gtcatcatct cttcgtgatc cagggccccc agcctgcgaa
     3781 tgccgccgac tatggcgcgc atgtgccgca cggcctcgac cacgttgccc gtgcccgcct
     3841 cgcccttgga ccggatcatg gccgccccct cgccgatgcg ccgcagcgcc tccccgaggt
     3901 tcgtggcgcc gcacacgaaa ggcacctcga agacggcctt gtctatgtgg tgcttctcgt
     3961 cggcgggggt caggacctcg gactcgtcta tgtagtccac ttcgagggcc tccaggatct
     4021 gggcctccac gaagtgtccg atgcgcgcct tcgccatcac ggggatggtg acggactcct
     4081 ggatctcgac gatcttctcc gggtcactca tcctcgcgac cccgccgtcg gcccttatgt
     4141 ccgcgggcac gcgctccagg gccatgacgg ccacggcccc cgcttcctcg gcgatcctgg
     4201 cctgctcggc gtcgacgacg tccattatga ccccgccctt gagcatctgg gccatcccgc
     4261 tcttgaccct gaaggtccct gttaactgtc cgttctcggc cataaaattc tccccttggt
     4321 atctactaga atatgtaaag gatatcttac taccggtgac ccggtgatcc aaactgtagt
     4381 aggtttcagg ttatgggatc aggagaacct tagttcttct tgcgttcctt ggcgtattcc
     4441 tccgagaact tcttgaagaa cgcctgggca gcgtggcttg cggcccgctt ggccgcgggg
     4501 agggccacgg tgaattcttg gcgggcgaac tcgcccactt tctggactac tcttgctgcc
     4561 gtcccatcct cccgtggtct ggccaggcgt ctcaggcgtc ccattactct atctctatgc
     4621 gctctcggtt cacggtttgt gaggagtcat gctgcctctc gggcctcacc agctctccga
     4681 cacccttcag cgtctcgaag cccgctttgc gaaagtgcgc tgcggcctgc acgcgccgct
     4741 caccgggcac gaggacgtag gcgatcacac ccagcagcgc gcccttgcgt cgtctctctt
     4801 cactcatcct tcaacctcac tttcagcgcc cctccctcga acctcgcccc ggtcgcagaa
     4861 agtcgcgcga gggcatccgg cagcaggatg ctccttcg
//
LOCUS       DATMVF010000274        12406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_223867, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000274 DATMVF010000000
VERSION     DATMVF010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12406
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223867"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VN178_14255"
     CDS             complement(<1..325)
                     /locus_tag="VN178_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82178.1"
                     /translation="MTTQERNRQGFWQSLFGRDSRQATPEEMPKPVVEAPSQEPGPEV
                     EIAPNDPIVAYFASASGVVEIDKLDLDSPALRAMKTADIKVVVPLVSQGELIGLLNLG
                     PRLSQQ"
     gene            655..1497
                     /locus_tag="VN178_14260"
     CDS             655..1497
                     /locus_tag="VN178_14260"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HWS82179.1"
                     /translation="MREIAASSVTETVRDLCIEANTRLPQEHLAALRRALEAEESELG
                     REVIERLLENAEVARERCIAFCQDTGYAVFFVELGSEVRFSGGDIGAAIDEGVRRGYE
                     EGYLRKSIVRSPVDRTNTGDNTPAVVYYEPVSGDGLRLTMLVKGAGCDNMSALKMLTP
                     AEGLPAMKRFVVETIERAGPNASPPLTVGVGMGGPFEKAAMLAKRALTRPSGEPNPDP
                     DLAVLERELLDEINATGIGPAGYGGTQTALAVHIESFPTHIAAFPVAVNLDCHSHRTA
                     SAEL"
     gene            1494..2060
                     /locus_tag="VN178_14265"
     CDS             1494..2060
                     /locus_tag="VN178_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HWS82180.1"
                     /translation="MSPEPVFLTAPLSMEDIEPLRSGDIVRLNGVLYTARDAAHARMK
                     DAIEADEPLPFDPEGQVVYFTGPAPARPGRALGPAGPTTASRMDPHSPMLVERGLRGM
                     VGKGSRSDRVLEAMREHGCVYFGAVEGTAALLADCVKEAEVVGYEDLGAEAVRRLVVE
                     DFPVVVVNDLYGGDLYKEGRERWRRSPG"
     gene            complement(2065..3447)
                     /locus_tag="VN178_14270"
     CDS             complement(2065..3447)
                     /locus_tag="VN178_14270"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HWS82181.1"
                     /translation="MATRKIQVERVQDLNEADVREGGHVLYWMQSSQRAEQNHALEYA
                     VQRANDLDQRLLVVFGLTDDYPEANLRHYTFMLEGLKDAQEAVEKRGIEMVVREGSPE
                     EVALEAGKDASIIVTDRGYMRPQKKWREKVAREADCLVTQVESDVVVPVELASGKQEH
                     AARTLRPKIQEYLEDFLVALRPTRVEKRSTKMQEDGLDLSDVEKVLDGMDLDRSVGAL
                     SHLYKGGNSEAKKIFRRFLKQGLGDYDANRNQPQTDYVSHMSKYLHFGHVSPIWLALQ
                     AREATAKQNNIESFVEELVVRRELSMNFVFYNNDYDSYSNLPDWARHTLEEHKGDERE
                     YTYTRKQLENAETHDEYWNAAMKEMVHTGYMHNYMRMYWGKKILEWSNTPEYAYKTTL
                     YLNNKYFLDGRDPNSFANVAWVFGQHDRGWTEREVYGKVRYMSSGGLERKTKPDQYVE
                     KVEERIEGSG"
     gene            complement(3575..3991)
                     /gene="msrB"
                     /locus_tag="VN178_14275"
     CDS             complement(3575..3991)
                     /gene="msrB"
                     /locus_tag="VN178_14275"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWS82182.1"
                     /translation="MTDTPRKIQKTEDEWQDQLTPEQYRITRMKGTERPFSGEYYDNK
                     EEGIYRCVACGTPLFASDTKYDSGTGWPSFWEPIDQANVETEEDRSLFMRRTEVHCAV
                     CGAHQGHVFEDGPAPTGQRYCINSAALRFDPEAASE"
     gene            complement(4287..4787)
                     /locus_tag="VN178_14280"
     CDS             complement(4287..4787)
                     /locus_tag="VN178_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWS82183.1"
                     /translation="MAIKEGWRGRFFEDFEVGDVYEHPLGRTVTTTDNLWFTLLTQNT
                     APIHVDHAYAAKTEYGLPLVDSTFTVALVTGQSVTDVSQNVFANLGWDEIRLPAPVFE
                     GDTIYSRSEVIEKRGSRSRPNVGIVRVKTTGYNQEGETVITFLRTVMVYRRDHAPQVP
                     RPTPEA"
     gene            4893..6038
                     /locus_tag="VN178_14285"
     CDS             4893..6038
                     /locus_tag="VN178_14285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS82184.1"
                     /translation="MTKKHEHLRRGVREVCSRFPNEYWRGLDAERRYPEEFVAAMTGA
                     GYLGALIPEEYGGMGLDLSAACAILEEVNLSGANAGPAHAQMYTMGTLLRHGSEEQKR
                     EYLPKIARGDLRLQAFAVTEPEAGTDTTSIRTTAKREGDHYVVNGRKIYISRVEQSDL
                     MILLARTTPLEQVEKRSSGLSVFLVDLREAEGLSVRPRRVMMNNATNELTFQGLRLPA
                     ENLIGEEGAGFRYIIDGMNAERILIAAECVGDGRWFVEQATQRASGREVFGRRIGENQ
                     GIQFPIARAYANVEAASLMVEKAAALFDRGEPCGAEANLAKLLAADASWEAANAALQT
                     FGGYGFDAEYGVERKFRETRLYQVAPISTNLILSYLAEHVLGLPRSF"
     gene            complement(6078..6608)
                     /locus_tag="VN178_14290"
     CDS             complement(6078..6608)
                     /locus_tag="VN178_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HWS82185.1"
                     /translation="MRREFGAVTVECIQGDITSQYDVEAVVNAANARLEPGGGVAGAI
                     HRAAGPGLAEEAVPLGPIRPGEAVITGAHGLPNRYIIHTLGPVYGQDRPEAELLAGCY
                     RNSLALAEENGIESIAFPAISTGIFGYPVEEAAEVALGTVRNGAERLERVHLIRFVLF
                     GESDLEVHERVLSGLP"
     gene            complement(6608..7753)
                     /gene="nagA"
                     /locus_tag="VN178_14295"
     CDS             complement(6608..7753)
                     /gene="nagA"
                     /locus_tag="VN178_14295"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HWS82186.1"
                     /translation="MRFVALRGQIVTDYEVWPDGTVLLGEGSIADVSPDDSLLAEADE
                     VHDHEGSLILPGFVDLQVNGAFGVDVATESARLPELSEALLSTGTTSYLPTVISSPEG
                     LYEEALPEIGTPTGSGAEVLGVHLEGPFISLQKRGAHAAAHVLMPDAELLAHLLDLGP
                     VRMITLAPELEGADKLTALAANRGVVVSAGHSDIAFEEAYDALDGQVASVTHLFNGMS
                     AMHHREPGLPGAAFAHPRAVCGLIADGLHVHPEMVGLAFRMLGPDRLCLVTDAIAATG
                     MEDGEYRLATRTVYTGGGVPRLGSGSIAGSMLTMKDAFMNILAFTGCTIPEAARMTST
                     SPAKLVGEGRRKGRLVPGYDADVTVLTPDLSVERVWRGGKLAYSWEG"
     gene            complement(7802..9151)
                     /locus_tag="VN178_14300"
     CDS             complement(7802..9151)
                     /locus_tag="VN178_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS82187.1"
                     /translation="MRERRLSGGKASRLAAFSWILYDLSNTIFSVTILSFYFPLWVEE
                     QVGSGGLLSGAGLVNSAAALSALFVVLTAPVLGAVADLRQRRVPYLILLTTIAVALTA
                     ALDVAGGVVVGLALFVAADLAYQSALVFYNALLPVVSAGRGAGRISGYGTAVGYVGTI
                     LGLVVLTYFVENGETLRPVLGPLGGWLEAGDEVYSNAFVPTAVIYLVFSLPAFFFVPD
                     PAVRARRPVVLGAVYRDVVSTVKNLRAYAGVGTFILATILYTDAANTAVANMALYGRV
                     VFEMDGTQVRNLLLFSTAFAVVGSAGFGLLTDRVGPKRSLVGVLVLWLVAILLSAAAL
                     GAWMLFLAGPIVGIALGGMWTVGRVMLVALSPPEKLGEFFGLFSLAGKVSAVFGPALT
                     AVFLFVFGGGAYGYRISVGSLAVIMALGLFFLLRVPDARPERNVDEFAGATLDSRAP"
     gene            complement(9210..10019)
                     /locus_tag="VN178_14305"
     CDS             complement(9210..10019)
                     /locus_tag="VN178_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWS82188.1"
                     /translation="MIGVLAGMSPVLQALLATMFTWGVTALGASLVFGARKVSRKLLD
                     AMLAFAAGVMIAASFWSLLAPSIDLSRGGPLPMWVPPLIGFLAGGIFLRGLDLVLPHL
                     HPGHPMKDAEGIHTSWRRSVLLVSAITLHNIPEGLAVGVAFGAAAAGIPGASIGAAVA
                     LAIGIGLQNFPEGMAVAMPLRREGLSRSRSFFYGQLSAAVEPVAGVIGAAAVVLARPI
                     LPYALAFAAGAMIFVVAEELIPEAKRGSPDIAALALMVGFAVMMTLDVALS"
     gene            10230..10916
                     /locus_tag="VN178_14310"
     CDS             10230..10916
                     /locus_tag="VN178_14310"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWS82189.1"
                     /translation="MNELSETRSPSSSVGDYLKAVWELAVDSGGAASTKGVAARLSVS
                     SASVSNMFARLQDMGLVRYERYRGATLTERGREEALRLVRRHRLIETFLLEHLDYDWQ
                     EVHDEAERLEHAVSDGFTERLAELLGHPDHDPHGDPIPSAEGTLEVEESFALSQAVAG
                     QRVRISKVSDEDAAMLDYLGDRNLVPGRRLSVREVRALDGVVVVEDEEAEVYALGEPL
                     ARSIFVRDDP"
     gene            11001..11903
                     /locus_tag="VN178_14315"
     CDS             11001..11903
                     /locus_tag="VN178_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWS82190.1"
                     /translation="MDQKGDTRGHTDKTIRIRAPEQVVVVDPPPEPAYPRYRVEPGQR
                     ASLATVDPNETEHYKKKKDVAKALEKQRRRIQDLQERLYAENERGMLIVLQAMDTGGK
                     DGTIKHVFSGVNPQGCRVSSFKAPSAEEANHDFLWRYHKSAPAKGRIGIFNRSHYEDV
                     LVVRVKNLVPEDIWSRRYDQINAFERNLSLGGIAVLKFFLHISKDEQKRRLQSRLDDP
                     DKRWKFSSNDIKERGFWDAYQQAFEDALSNCSTEHAPWYVVPANKKWYRNLVVARTIA
                     DTLEAMDPRYPPAEEGLEKVKIPD"
     gene            12165..>12406
                     /locus_tag="VN178_14320"
     CDS             12165..>12406
                     /locus_tag="VN178_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82191.1"
                     /translation="MDTLPAGVHRLLFPAWGLVMGGVSEARPWWHLWVVCCLLEVSTV
                     ACGVPPEESSDSGSPAEETRVVAEHEAGHATEEADL"
BASE COUNT         2319 a         3958 c         3958 g         2171 t
ORIGIN      
        1 cctgctggga gaggcgcggc cccaggttga gaagcccgat caactctccc tggctcacca
       61 gcggtacgac caccttgatg tcggccgtct tcatggccct gagggcgggc gaatcgaggt
      121 caagcttgtc gatctcgacc acccccgagg cgctggcgaa gtaagccacg atggggtcgt
      181 tgggcgctat ctccacctcg ggaccgggct cctggctggg agcctccacc acgggtttgg
      241 gcatctcctc gggtgtcgct tgcctactgt cgcgcccgaa gagactctgc cagaaaccct
      301 gcctgtttct ctcctgtgtc gtcatcctac ctcctggccc tgattctagc ccccaaacgt
      361 ctcccagacg ttacgaaaag cgttacgaaa agctccccgt cggaaatctc cccagtaatt
      421 gtctcacgtc tcgatttgta cgtcgcctga cctgtgggca cagtccgctc tcgggccgca
      481 cgatgctccg gatgaagcgt atcccaggcc atctgcacgc tacccggtga acgtctcgca
      541 atcgttacgc aaagcgctac gagaatcgtt gctcgaataa cctcgaccga caacgtggaa
      601 aggaggacgc ctctgatgct aacctttgcc cgatgaggtc gaagcaataa cccaatgcgc
      661 gagatagcgg ccagttcggt aaccgaaacg gtgcgcgacc tgtgcatcga ggccaacacg
      721 cgtctgccgc aggagcacct ggccgccctg aggcgcgcgc tcgaagcgga ggagtcggaa
      781 ctcgggcgcg aggtgatcga acgcctcctg gagaacgcgg aggtcgcccg cgagcggtgc
      841 atcgccttct gccaggacac gggatacgcc gtcttcttcg tcgagctggg gagcgaagtc
      901 aggttctcgg gcggagacat aggggccgcc atagacgagg gcgtccgccg cggctacgag
      961 gagggctacc tcaggaaatc catcgtcagg agccccgtag acaggacgaa cacgggcgac
     1021 aacaccccgg ccgtcgtcta ctacgaaccg gtctccggcg acggtttgag actcacgatg
     1081 ctggtcaagg gcgcgggctg cgacaacatg agcgcgctca agatgctcac ccccgcggaa
     1141 ggcctccctg cgatgaaacg gttcgtcgtc gagaccatcg agagggcagg cccgaacgcc
     1201 agcccgccgc tcaccgtcgg ggtcggtatg ggcggcccgt tcgagaaggc tgcgatgcta
     1261 gccaagaggg cgctaacccg gccttccggc gagcccaacc ccgaccccga tctcgccgtc
     1321 ctcgagcgcg agctactcga cgagatcaac gccacaggca taggacccgc cggctacggc
     1381 ggtacgcaga cggcgctcgc ggtccacatc gagagcttcc cgacccacat cgccgccttc
     1441 cccgtcgccg tcaacctcga ctgccactcc caccgtacgg cctcggccga gctgtgagcc
     1501 cagagccggt cttcctgaca gcgccgctct cgatggaaga catcgagccc ttgaggtcgg
     1561 gcgacatcgt gcgcctgaac ggcgttctgt acacggcccg cgacgcggcc cacgccagga
     1621 tgaaggatgc gatcgaggcg gacgagccgc tccccttcga ccccgaaggg caggtcgtgt
     1681 actttacggg cccggctccg gcgcgcccgg gtcgagcgct gggacctgcg ggaccgacga
     1741 cggcatcgag gatggacccg cactcgccga tgctcgtaga acgtggtctg aggggcatgg
     1801 tcggcaaggg ttcgcgctcc gacagggttc tcgaagccat gagggagcac ggctgcgtgt
     1861 acttcggagc cgtcgagggt acggcggcgc tgctggcgga ttgcgtcaag gaggccgagg
     1921 tcgtcggcta cgaagacctg ggtgccgagg cggtccggcg cctcgtggtc gaggacttcc
     1981 cggtcgtcgt agtgaacgat ctctacggag gcgatcttta caaggaaggc cgcgagcgct
     2041 ggaggcgttc gccgggctga ggtcctagcc ggacccttcg atcctctcct cgaccttctc
     2101 gacgtactga tccggcttcg tcttgcgctc caggccgccc gaggacatgt agcggacctt
     2161 gccgtagacc tcgcgctcgg tccagccgcg gtcgtgctgg ccgaagaccc aggccacgtt
     2221 ggcgaacgag ttcggatcgc ggccgtcgag gaagtacttg ttgttgaggt agagcgtggt
     2281 cttgtatgcg tactcgggcg tgttcgacca ctccaggatc ttcttccccc agtacatgcg
     2341 catgtagttg tgcatgtagc cggtgtgtac catctccttc atcgccgcgt tccagtactc
     2401 gtcgtgcgtc tctgcgttct cgagctgctt tctggtgtac gtgtactctc gctcgtcgcc
     2461 tttatgctct tcgagcgtat gcctcgccca gtctgggagg ttggagtacg agtcgtagtc
     2521 gttgttgtag aagacgaagt tcatggacag ctcgcgcctc acgacgagct cctcgacgaa
     2581 ggactctatg ttgttttgct tcgccgtcgc ctccctggcc tggagggcga gccagatcgg
     2641 ggagacgtgc ccgaagtgca ggtacttgct catgtgcgag acgtagtcgg tctgcggctg
     2701 gttgcggttc gcgtcgtagt cgccgagacc ctgtttcaga aagcggcgga agatcttctt
     2761 ggcctccgag ttgcccccct tgtacaggtg gctgagggcg ccgacgctac ggtcgaggtc
     2821 catcccatcg aggaccttct ctacatccga gagatccagg ccgtcttcct gcattttcgt
     2881 cgaccgcttt tcgaccctgg tcggcctgag cgcgaccagg aagtcttcga ggtactcctg
     2941 gatcttcggc cggagcgtcc gcgccgcgtg ctcctgcttg ccggaggcga gctcgacggg
     3001 cacgaccacg tcggactcca cctgtgtaac gagacaatcc gcctctctgg cgaccttctc
     3061 acgccacttc ttctgcggac gcatgtaacc gcggtccgtg acgatgatcg aggcgtcctt
     3121 gcccgcctcg agagctacct cctctgggga gccctcgcgg acgaccatct cgatgccgcg
     3181 cttctctact gcttcctgcg cgtccttcaa accctccagc atgaaagtgt agtggcggag
     3241 gttggcctcg ggatagtcgt cggtgaggcc gaacaccacg agcagacgct ggtccagatc
     3301 gttcgcccgc tgcacggcgt actcgagcgc gtggttctgc tcggcgcgct gggaggattg
     3361 catccagtac aggacgtggc ccccttccct tacgtccgcc tcgttcaggt cctgtacacg
     3421 ctctacttgt atcttccgcg tcgccatctg gcacctgcct ccgtagttct cgcctggaag
     3481 ctagattagg cgaagccggt ctcgacggat ggatcagaaa acacaaaagg acgccccctg
     3541 caacgaggca gggggcgtct tcttgtttcg aatcttactc tgaggcggct tcggggtcga
     3601 agcggagggc ggcggagttt atgcagtagc gctgccccgt gggggctggt ccgtcctcga
     3661 agacgtggcc ctggtgggcg ccgcagacgg cgcagtgtac ctccgtacgg cgcatgaaga
     3721 ggctacgatc ctcctccgtc tccacgttgg cttggtcgat cggctcccag aagctgggcc
     3781 agcccgtgcc cgagtcgtac ttggtgtcgg acgcgaagag gggcgtcccg caggctacac
     3841 accggtagat cccctcctcc ttgttgtcgt agtactcgcc gctgaagggc cgctccgtgc
     3901 ccttcatccg cgtaatgcgg tactgctcgg gggtgagctg gtcctgccat tcatcttctg
     3961 tcttctgaat cttccttggc gtatcggtca tgccgaaatt ttagcacgcc tgcttcgcag
     4021 gcttgtcagc acggcgcttc gcgccttgtc agcacgcttc ggcttcgcct cgctggtcag
     4081 caagtcagct tctcccgttc cggcctcgcc ctgccgcgca cgaggaaccg ctacctgtgg
     4141 cggtcggatt ctacgctctt ggccacgctt cgagacgact agctgaagtg ctgacgagcg
     4201 aaggcgtaag cctgagcgtg ctgacttgct gaccagagcg gattcaggaa gggtgaccct
     4261 tcctgaatcc gctctcagtt caggattcat gcctcaggag tcggacgggg cacttggggc
     4321 gcgtgatccc gcctgtagac catgacggtt cgcaggaacg tgatgaccgt ctcgccctcc
     4381 tggttgtagc ccgtggtctt caccctcact atgcctacgt tcggccgcga gcgcgaccct
     4441 cgcttctcga tgacctcgct cctggagtag atcgtatccc cctcgaagac gggcgcgggg
     4501 agccggatct catcccaccc gaggttggcg aagacgttct gggagacatc cgttacgctc
     4561 tggccggtca ccagggcgac ggtgaaggtg gagtcgacca gcggaaggcc gtactcggtc
     4621 ttggcggcgt aggcgtggtc cacgtggatg ggggccgtgt tctgggtgag gagcgtgaac
     4681 cacaggttgt ccgtggtcgt caccgtgcgc cccaggggat gctcatagac gtcccccacc
     4741 tcaaagtcct cgaaaaagcg tccccgccac ccctctttga tcgccatgct actccccctg
     4801 tattcgggct atggtaaagt cgatttcgtg atcatcgtct cgcagatagg cccggaatgc
     4861 tacccggtcc cagcctggtg ggaccctata tcttgacgaa aaagcacgag catctccgcc
     4921 ggggcgttag ggaggtatgc tcccgcttcc cgaacgagta ctggcgcggt ctcgacgcgg
     4981 aacgccgcta tcccgaagag ttcgtcgccg cgatgacggg tgccggctac ctcggtgccc
     5041 tgataccgga ggagtacggt gggatggggc tcgacctctc tgccgcttgc gccatcctcg
     5101 aggaggtcaa ccttagcggc gccaacgccg gaccggccca cgcccagatg tacaccatgg
     5161 gcacactcct caggcacggc tccgaggagc aaaagcgaga gtacctgccg aagatagcca
     5221 ggggagatct gagactgcag gccttcgccg tcaccgagcc ggaagccgga accgacacga
     5281 cctccattcg caccacggcg aagcgcgagg gagaccatta cgtcgtgaac gggcgcaaga
     5341 tctacatctc cagggtcgaa caatctgacc tcatgatcct gctcgcccgc acgacaccct
     5401 tagagcaggt cgaaaaacgc tccagcggtc tatccgtctt cctcgtcgac ctgagggagg
     5461 cagaaggcct cagcgtaaga ccgaggcgcg tgatgatgaa caacgcgacc aacgagctaa
     5521 ccttccaagg cttacggctc cctgcggaga acctcatcgg cgaagaggga gcaggtttcc
     5581 gctacatcat cgacggcatg aacgccgagc gtatcctcat cgccgccgag tgcgtgggcg
     5641 acgggaggtg gtttgtggag caggccacgc agcgcgcttc ggggcgcgag gtgttcgggc
     5701 gcaggatcgg cgagaaccag gggatccagt tccccatagc gagggcctac gcgaacgtcg
     5761 aggccgcctc gctgatggtg gagaaggcgg cggccctctt cgaccgggga gaaccgtgcg
     5821 gcgccgaggc gaacctggct aagctcctgg ccgccgacgc ctcttgggag gcggccaacg
     5881 ccgcccttca gaccttcggc ggctacggct tcgacgccga gtacggcgta gagcgcaagt
     5941 tccgcgagac gcgtctctac caggtagccc cgatctcgac gaacctcatc ctctcttact
     6001 tggccgagca cgtcctcggc ctccccaggt ctttctgaca gcgagatccc ttgcaacccg
     6061 cggcgcaaac gccgactcta cggcagcccg gagaggaccc tctcgtggac ttcgaggtcg
     6121 ctctcaccga agaggacgaa ccgtatgaga tgtacccgct cgagcctctc cgctccattt
     6181 cgcacagttc cgagcgccac ttccgctgct tcctcgacgg ggtagccgaa gataccggtc
     6241 gagatggcgg ggaaggcgat ggactctatc ccgttctcct cggcgagtgc gagtgagttg
     6301 cggtagcagc ccgccaggag ctcggcctcg gggcggtcct ggccgtagac cggtcccaaa
     6361 gtgtggatga tgtaacggtt cggaagcccg tgcgcaccgg ttatgaccgc ctcgccaggc
     6421 cgtatggggc cgagcggcac ggcttcctcg gcgagaccgg gtccggcggc gcggtggatg
     6481 gcccctgcga cccctccgcc gggctcgagc cgcgcgttcg ccgcgttgac cacggcctcc
     6541 acatcgtact gggaggttat gtctccctgg atgcactcga ccgtaacggc cccgaattca
     6601 cgtctcatca gccctcccag gagtaggcca gcttgccacc ccgccacacc ctctccacgg
     6661 agaggtcggg cgtcaggacc gtaacgtcgg cgtcgtagcc tgggacgagg cggcccttgc
     6721 ggcgaccttc gccgacgagc ttcgccggtg aggtggaggt catacgcgcg gcctcgggga
     6781 tggtacagcc ggtgaaagcc aggatgttca tgaacgcatc tttcatggtc agcatgcttc
     6841 cggcgatgga accgctcccc agtctcggga cgccgccgcc agtgtacacg gtgcgggttg
     6901 ccagacggta ctcgccgtcc tccatgcccg ttgcggctat ggcgtcggtg acgagacaga
     6961 ggcggtcggg accgagcatg cggaaggcca gcccgaccat ctccggatgg acgtgcaacc
     7021 cgtcggcgat cagaccgcag actgccctcg ggtgggcgaa ggcggcccct ggcagcccag
     7081 gctcgcggtg gtgcatcgcg ctcatcccgt tgaagaggtg tgtgacgctg gcgacctgcc
     7141 catccagagc gtcgtaggcc tcttcgaagg caatatcgga gtggccggcc gagacaacca
     7201 ctccacggtt cgcggcgagg gcggtgagtt tgtcggcgcc ttccagctcg ggagccagcg
     7261 tgatcatacg cacgggcccg aggtcgagca gatgggcgag caactccgca tcgggcatca
     7321 ggacgtgggc agccgcgtgg gcacccctct tctgcaggct tataaacggc ccttcgaggt
     7381 ggacgccgag cacctcggcg cccgacccgg tgggggtgcc gatctcaggc agggcctcct
     7441 cgtagaggcc ctcgggcgac gagatgaccg tgggcaggta tgaggtggtg cccgtcgaga
     7501 gcagggcttc ggagagttcg ggcaggcgcg cactctccgt ggccacgtcg acaccgaagg
     7561 ccccgttgac ctgcaaatcg acgaagccgg gcaggatcag ggacccttcg tggtcgtgga
     7621 cctcatccgc ttcggcgagg agagagtcat cagggctcac gtccgcgatg gacccttcac
     7681 ctaggaggac cgtgccatcg ggccatacct cgtagtccgt aacgatctgg ccccgcagcg
     7741 cgacgaacct catgttacgg ttctccgaat atcctcgccc ctcatagcac acatcttccg
     7801 ctcacggggc ccgcgaatca agggtggcac ccgcgaactc gtcgacgttg cgttccgggc
     7861 gggcgtcggg gacacgcagg aggaagaaga ggcccaaggc catgatgacg gcgagcgagc
     7921 ctacggagat cctgtagccg taagcgccgc ccccgaagac gaagagaaac acggccgtga
     7981 gcgcgggccc gaagaccgcg gagaccttcc cggccagcga gaagagaccg aagaactccc
     8041 ccagcttctc gggcggcgag agggcgacga gcatcacccg ccccacggtc cacatccctc
     8101 ccagcgcgat ccccacgatg ggccccgcga gaaacaacat ccacgccccc agggcagcgg
     8161 cggagagcaa gatcgcgacg agccacagga cgagtactcc gacgagcgac ctcttgggtc
     8221 ccacccggtc cgtaaggagg ccgaaaccgg ccgagccgac cacggcgaag gccgtggaga
     8281 agaggagcaa attccttacc tgcgtgccgt ccatctcgaa gaccacccga ccgtagagcg
     8341 ccatgtttgc gacggcggta ttcgccgcgt cggtgtagag gatcgtagcc aggataaagg
     8401 tccccactcc tgcgtaagcg cgcaggttct tcacggtgct gaccacgtca cggtagaccg
     8461 ccccgaggac cacgggcctc cgcgcccgca ccgcagggtc gggcacgaag aagaaagcgg
     8521 gcagcgagaa gacgaggtag atgacggcag tcggcacgaa ggcgttggag tagacttcgt
     8581 caccggcctc cagccatccc ccgagtggcc caagcaccgg acgcagcgtc tctccgttct
     8641 cgacgaagta cgtcagaaca acgaggccga ggatggtgcc aacgtagccc actgcggtgc
     8701 cgtagccgct gatcctgccg gccccgcgtc ccgccgagac gacgggcaag agcgcgttgt
     8761 agaagacgag cgcggactgg taggcgaggt cggccgcgac gaacagcgcg agccccacca
     8821 cgaccccgcc ggccacgtcg agcgcggcgg tgagcgcgac cgcgatagtg gtaagcagga
     8881 tcaggtaagg cacccgtcgc tgtctgaggt cggctacggc cccgagaacg ggcgccgtga
     8941 gcacgacgaa cagcgcggag agggccgcag ccgaattgac gaggccggcc ccggagagga
     9001 ggcccccgct cccgacctgc tcctcgaccc agagcgggaa gtaaaaggag aggatggtga
     9061 ccgaaaagat ggtgttcgag aggtcgtaga ggatccagga gaaagccgcc agcctcgagg
     9121 ccttgccgcc ggataacctc ctctctctca tcccggcggc agtatagagt acgcggccga
     9181 cgtttagatc atctcgggca ttcgcctccc taactgagtg cgacgtcgag ggtcatcatg
     9241 acggcgaagc cgaccatgag ggcgagggcg gctatgtcgg ggctgccgcg tttcgcctcg
     9301 gggatgagct cttcggcgac gacgaagatc atggccccag cggcgaacgc cagagcatag
     9361 ggcagtatcg gcctggcgag cacgaccgca gccgccccga taaccccagc aacgggctcg
     9421 accgccgccg agagctgccc atagaagaag ctcctggatc tcgacagccc ctcacgccgc
     9481 agcggcatgg ccacggccat accctcgggg aagttctgaa gccctatgcc tatggcaagc
     9541 gcgaccgcgg cgccgatcga agccccaggg atgcccgccg ccgccgctcc aaaggccact
     9601 cccaccgcaa gcccctcggg tatgttgtgg agcgttatag ccgagacgag caacacgctc
     9661 cggcgccagc tcgtatggat gccctccgcg tccttcatcg ggtggccggg atggaggtgc
     9721 gggagtacca gatcgaggcc ccgcaggaaa atcccgccag ccagaaaccc gatgaggggc
     9781 ggcacccaca tcggtagcgg tcctccccgg gagagatcta tggaaggcgc cagcagcgac
     9841 cagaaactgg ccgcgatcat gaccccagca gcgaaagcca gcatcgcgtc gagaagcttg
     9901 cggctgacct tcctcgcgcc gaacaccagc gacgccccga gcgccgtaac cccccaggtg
     9961 aacatcgtgg ccaaaagcgc ctgcaagacg ggagacatcc cagcgagaac cccgatcacc
    10021 cgcccaacct actctcgtgc cccgtgttgc cttcgatggt gaacgcactc accccctcga
    10081 ttacttcgtt cacttcgctc gttcttccat tcatggtacc ctcccaaggc ttgaatttag
    10141 ctttggctaa ctttatattg aactacggta aataatcaag gcgctctgct cttgccaatg
    10201 aatctgcata gtctactatg gtcttcgaga tgaacgaatt gtcggagacc cgttcccctt
    10261 cttcttcggt cggggactac ttgaaggctg tttgggagct cgctgtggat tcggggggtg
    10321 ccgcttcgac caagggcgtg gcggcgcggc tctccgtatc gtcggcctcg gtctccaaca
    10381 tgttcgcgag gttgcaggat atgggccttg tgaggtacga gcgctacagg ggggcgacgc
    10441 tcaccgagcg cggtcgagaa gaggccttac ggctggtacg gcggcaccgg ctgatcgaga
    10501 cgttcctcct cgagcacctc gactacgatt ggcaggaggt acacgacgag gccgagcggc
    10561 tggagcacgc ggtctcggac gggttcaccg agcgtctggc ggagctgttg gggcatccgg
    10621 atcacgaccc gcacggggac ccgatcccct cggccgaggg cactctcgag gtggaggaat
    10681 cctttgcttt gagccaggcc gtcgcgggcc aaagggtgcg tatctcgaag gtaagtgacg
    10741 aagacgctgc gatgctcgac tacctgggtg atcgcaacct cgtcccaggg aggcggttga
    10801 gtgtcaggga ggtgcgggcc ctcgacgggg tcgtggtagt cgaggatgag gaggcggagg
    10861 tctacgccct cggggagccg ttggcgcgtt cgatctttgt cagggacgat ccgtagccta
    10921 ctcctctcaa aagggttcgg gcggctttcg cgcttgattt ctgagaaacc gggtatctta
    10981 cccgtacggg aacgaggatt gtggatcaga agggcgacac gcggggacac accgacaaga
    11041 ctattcggat cagggcaccg gagcaggtcg tggtggtaga tccaccaccc gagcccgctt
    11101 acccacgcta ccgggtcgag ccgggccaaa gggcatctct agcgacggta gatcccaacg
    11161 agaccgagca ttacaagaag aagaaggacg tcgccaaagc gttggagaag caacgcaggc
    11221 gcatacagga cctccaggag cgtctctacg cggagaacga gcggggaatg ctgatcgtgt
    11281 tgcaggccat ggatacgggt gggaaagacg gaaccatcaa acacgtcttc agcggtgtca
    11341 acccgcaggg atgcagggta tcgtccttca aggccccgag cgcagaagaa gccaaccacg
    11401 acttcctgtg gcggtaccac aagagcgccc cggcaaaggg caggatcggc atcttcaacc
    11461 gctcccacta cgaagacgtg ctcgtcgtcc gagtcaagaa cctggtcccg gaggacatct
    11521 ggagtcggcg ctacgaccag atcaacgcct tcgagcgtaa cctctcgctc ggcggcatag
    11581 ccgtcctcaa gttcttcctg cacatctcca aagacgagca gaagaggcga ctccagtcgc
    11641 gtctcgacga cccggacaaa cgttggaagt tctcctcgaa cgacatcaag gaacgcggct
    11701 tctgggacgc ataccagcag gcattcgagg acgccctcag caactgctcg accgagcacg
    11761 ccccctggta tgtggtgccg gccaacaaga agtggtaccg caacctcgtc gtcgcccgca
    11821 ccatcgcgga caccctcgag gcgatggacc cgcggtatcc tccagccgag gaagggttgg
    11881 agaaggtaaa gatcccggat tgaagtaagt ccatgaacag ggagtggcga cacgaggggt
    11941 gcgtcatcag caacgacggt tcacgcctgg aaatagtcca tagttaggag tttgctgttc
    12001 ttccgatacc aagtccgggt gaataccaat gccagcagaa agtcctccaa ctacccaccg
    12061 ctctactgct cgtgaattcc cgctccccgc tttcgcgggg acaagcttcg cgggaatggc
    12121 agcaatacgg tccccttcaa acggacccgg tatgatgcga tagagtggac acgttaccgg
    12181 ctggtgtcca caggctactc tttcctgcat gggggttggt gatgggcggg gtgagtgagg
    12241 cacgcccgtg gtggcatctg tgggtggttt gttgtctgct ggaggtatct acggttgcat
    12301 gcggcgtccc gccagaagag agctcggact ccggaagtcc ggcggaggaa acacgggtgg
    12361 tcgccgaaca tgaagccgga cacgctacag aggaggccga cttgag
//
LOCUS       DATMVF010000275        14015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_588202, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000275 DATMVF010000000
VERSION     DATMVF010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588202"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VN178_14325"
     CDS             <1..580
                     /locus_tag="VN178_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS82192.1"
                     /translation="MLDALGEYERAEEAYQRAIASGHSEEAPRAAFDLGLMLDALGEY
                     ERAEEAYQRAIDSKHPEEAPRAAFNLGLMLNEQGEYERAEEAYQRAIDSEHAEWAPKA
                     MFNLGMLFEERGERDLAAKAYQRAVESGHSDAASKARLNLGVLFEQTGEYDLAEEAYQ
                     QAIDSGHPEVAPEGMRNLRGLSTRFPKKIRRG"
     gene            1015..1695
                     /locus_tag="VN178_14330"
     CDS             1015..1695
                     /locus_tag="VN178_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82193.1"
                     /translation="MEEQTRNGAPAQQGTSAAASSNLGVTLLRVAWLAILLGMAMEGI
                     LLLLSAGLGDLLGLGSIVAELARNVSWSLLVCLGLSVGKAVQSARVPVMGLLGFLVAP
                     AAFEISRVIHKGAIQALAISGSAGEDLSPFLLALIKGLEYGCLGLALGWVGQRPWGGA
                     MAHMAVGFVVGAVFGGIVIALLAASGPEVSATILLSRGVTEVLFPVGCSLVLFSAEAL
                     GTSMASSD"
     gene            2159..3142
                     /locus_tag="VN178_14335"
     CDS             2159..3142
                     /locus_tag="VN178_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82194.1"
                     /translation="MGAPAQEAHLNHGDELGACGDTATTTTSTTSTTTDTTTDTTTTT
                     DTTTGTTTTTGTATGTTTGTTGTCITGTTTGTTTGTSTTGTTDTTGTTTGTTGTTDTT
                     TDTTGTTTDTTGTTDTTGTTDTTGTTTGTTGTTGVCTPGTTDTTTGTTTGTTDTTGTS
                     TTGTAETTGTTGTTTGDTTTTGDGGDQEKVTLCHVSANGTETIEVDLSAQAAHLAHGD
                     TLGACEQTTGTTGTTDTTGTTGTTGTTGTTTGGETPTPGDGGDREKVCVRFKNDDNKK
                     YRWVSEGKEHRGDKVVKDKFCEQKKKGGHKGNDHNAHAEKNGDSGRGNDDD"
     gene            4176..4844
                     /locus_tag="VN178_14340"
     CDS             4176..4844
                     /locus_tag="VN178_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82195.1"
                     /translation="MEKGPLAGGTAIGRDVLVPGDVIDILPDGTRVNGIDQIIIETEN
                     CELTAQGSDLTITMSDRGVPFRIRDGDNADITLESDGTIVANGRSTLGESFPEAVRDN
                     PDKLIVPIPVDPENDQFPRGPNDTFPILSSTGVGGEGCRVVGNSRDGGGDAGQVDRGA
                     SIDDTITNAGDLNSKDGVIPDTISNNAVPNTGGMPMLGPVVSGLVFVGVGLLLFRSAV
                     RRNT"
     gene            4883..6574
                     /locus_tag="VN178_14345"
     CDS             4883..6574
                     /locus_tag="VN178_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82196.1"
                     /translation="MLPVEILREAVGSYAPYLQASGEMHDPVFGEPTQYGTPYHAFCN
                     AVLAREDGGERRWAYADRAVRGLEAALDHVLDPGLPPTASGFDGATGAVDRSNHRDFF
                     WPPILKTFLILRELGVDGADGLAARISGVDIGAAFAQRPPSNWAVVWLSGEWLRLREG
                     LSPFSMERLDLWLDTFFETHILSEQGLYQEPGHPNSYDLFTRYHLADMLAEGYDGRRR
                     EDLEILMRTGLLRSLGVQLSDGSLASAHRSTGQTWTLGAQCAFFTHASNHFRGQEPQL
                     AGRAEEAAYRALSSFRRWQRDGPYSPVENRLPPGHRVGYERYTADGHYANLAAAFLAA
                     AVSNGLNRPFTERSGGREASLLLEHDPTYRAVLHNGLYSLHFNARPAPDYDAFGIVDV
                     TFGPGRFLQFVSSAKHLESGRLLNLGMAGSSGSSQADLALTGRMQGGLAPPRLELEAR
                     NRGSPYRYRFAARVETEGVYVEETTPGLEDPKSLLIPYLRDPGTGTVTAAKVEAGRVR
                     LVHGREVVEISYGAPVKRVVHLTGGLQSRRGICGMLRIGFRERSQGISYRIRATR"
     gene            complement(6591..8411)
                     /locus_tag="VN178_14350"
     CDS             complement(6591..8411)
                     /locus_tag="VN178_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HWS82197.1"
                     /translation="MLGRHDPASIERALNEVSPPPPFPPASDRAAWEKVREAVGEQRV
                     SELLTRAETDARAEIPGLTATLYLELSRAGSREGYEEPANRRREMLSNLALAECLEGY
                     GRFLDPLLDVAWAICEESSWSWPAHQGDLADMNRPVIDLSVAMTALYLAELDLLLGDR
                     LDPALGRRIRHEVDRRCLVPYLERHDHPWLFDSPDRPVNNWTAVCNAGVAGAAIYLEP
                     DAARLAEILARSTRSLDDYLSTFDQDGGSTEGPAYWSYGFGYYTVLAHLVGHRTGWKV
                     DFFEGEHLRRISRFPLCTRLSPGLYVNFSDCDVNAGFVPAHLSYLSRRLELPDLARLL
                     EEQPGGDERELTWALRDLLWRPEHVEGSFVPARHDWFGGMMWMVSRQRPEDPGALVLA
                     TKGGHNGEMHNQNDVGNVIVHVDGESVISDIGRGRYTKAYFGPERYEHIANSSRGHSV
                     PVPNGQEQLPGTEHGARLLEHRSGDHADLAAFELRDVYPPEADLDSLRRTVVLRREEP
                     GANVELTDEVRFATGPGTFESVLTTFGEAEISPDSVLLRGVRGALRVFFDPEAVELRV
                     ETIEDVDLAEGPADVRRVFFAFLEPAREGSVHLRIDPESG"
     gene            complement(8576..10231)
                     /locus_tag="VN178_14355"
     CDS             complement(8576..10231)
                     /locus_tag="VN178_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82198.1"
                     /translation="MPKGEGLLNGAVYSDVHSMLGLSWKGPDYHTRVPQGAWVHPTRE
                     SLDYALTLLRSGGKKRAAAIVSRVLSLQDTDPTSKTYGIWPWLLEEPLTGMDPPDWNW
                     ADFCGARLAQLLVEHAADLPESLVGATQTGLGHAAAAIFRRNVGPAYTNIAVMGAGVS
                     LAAGELLDEPRFADYGRRRLRNIVEHTEHHGGFNEYNSPTYTVVTLAECERMLQLVRD
                     PSARADAERLRGAAWQTIAEHYHPGTSQWAGPHSRAYSDRLDAGIAGYLSDRTGVEIR
                     PHPAAVLRDRFEGTTPLPCPAGMVDRFRSPASEETELQSTFIRRGGDAGTTTGTTWMS
                     EGACLGSVNRDNLWDQRRVVLGYWRTEEEPAVVLRLRFLHDGRDFSSACARNGQSGPR
                     ILTAVGLLSGMGDAHHQLDVPASGTFHAEDFRVRYQLLGEGVAGKELGDGRFELSAGS
                     HRVVIHTTPGLFGPHELAWEMEQAEGRVCLDGVCYRGPRRNFDPAGLGKVVVVAGLEL
                     LRTDQPPSAHGPAVRETDGTLEATWPVGRGLEIQAPTHARPYQ"
     gene            complement(10272..11336)
                     /locus_tag="VN178_14360"
     CDS             complement(10272..11336)
                     /locus_tag="VN178_14360"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWS82199.1"
                     /translation="MEAEGAERNGAGGDALLEVKHLKMHFPIKQGFFGRTVGHVKAVD
                     DVDLKIRRGETLGLVGESGCGKTTLGRCIVRSYEPTEGEMVYHNRDGGTADLAKLGST
                     ALKPYRREIRMVFQDPNSSLNPRMTLLQIVGEPLKVNKVASGKELEDRVAELLRRVGL
                     RPEYMSRYPHAFSGGERQRVSIARALALDPRFVVADEAVSALDVSVRAQILNLLQDLQ
                     EEFDLTYLFISHDLSVVEHICDRVAVMYVGKVVEVAETGEIFDEPKHPYTEALLSAVP
                     KPDPRLRDRGRRIVLGGDVPDPANPPSGCYFHTRCPYAEDRCKDEEPPLRELTQGRYA
                     ACHFAEDLDLRGVGDQGAVR"
     gene            complement(11326..12495)
                     /locus_tag="VN178_14365"
     CDS             complement(11326..12495)
                     /locus_tag="VN178_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS82200.1"
                     /translation="MPDKSMDSNDSLLEVRGLKTHFFLDEGVVKAVDGVDLRVKRKET
                     LCVVGESGCGKSVTARSILQLVDRPGRIVEGEILFSPTPRTESNGKVEARKEGSATAT
                     EVREVLDLASLDPRSKTMRDIRGKEISMIFQEPMTSLSPVHTIGEQIMENILLHLPMD
                     KGEARERTVEELARVGIPNPGQRVDAYPFQLSGGMRQRAMIAMALSCDPSLLIADEPT
                     TALDVTTQAQILELMRGLQDELGMAIMFITHDLGVVAEMADDVAVMYLGMVAERGDVD
                     SIFHDPKHPYTRALLRSIPRMGRNKANQRLDSIRGMVPHPYNRPTGCPFHTRCDEIME
                     GYCEEILPPRIPLSSSGEPVGNEHDPSPASDQPLKDTREVRCLLYGGKEGSKDGG"
     gene            complement(12485..13642)
                     /locus_tag="VN178_14370"
     CDS             complement(12485..13642)
                     /locus_tag="VN178_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS82201.1"
                     /translation="MTEPPQPQVQEEQEARQRATDLGEKEAEIYVASQWKLVWWRFRK
                     HRLAVVGTVVVILIYLIALFAEFVAPFTPDAFDADYTYAPPQRLHFFDEDGFGLYVYG
                     YSSKLNEETLVRTFQVDESKKVPVRLFAHGNTYKLWGLFESDRHLIGPVEKGQPMYLL
                     GANDQGQDMLSRIIFGARVSMSIGLVGVALSFVFGVFLGGISGYYAGATDTAIQRLIE
                     VLMSIPTLPLWLGLSAALPPDWSPLRIYFGITIILSLIGWTDLARVVRGRFLSLRGED
                     FVTAAELDGSSKPRIILRHMLPSFASHIIASLTLAVPAMILAETALSFLGLGLQPPIV
                     SWGVLLQEAQNIRSVATAPWLLLPGFAVIIAVLALNFMGDGLRDSADPYAR"
     gene            complement(13652..>14015)
                     /locus_tag="VN178_14375"
     CDS             complement(13652..>14015)
                     /locus_tag="VN178_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS82202.1"
                     /translation="IRIVRANLLDELHKPYVTTARSKGVPERRLLVKYPVRVALNPFV
                     STVGWVLPLLLSGEVIVAQVLGLQTTGPMLLRALLSQDMYLAGSIILMISVLTVIGTL
                     ISDILLAWLDPRIRYQYQ"
BASE COUNT         2628 a         4337 c         4582 g         2468 t
ORIGIN      
        1 gatgctcgat gcgctaggcg agtacgagcg ggcagaggaa gcctaccaac gggcgatcgc
       61 ctcggggcac tcggaggaag ctcctagggc agcgttcgac ctgggcttga tgctcgatgc
      121 gctaggcgag tacgagcggg cagaggaagc ctaccaacgg gcgatcgact cgaagcatcc
      181 ggaggaggca cctagagcgg cattcaactt gggcttgatg ctcaatgagc aaggcgagta
      241 cgagcgggca gaggaagcct accaacgggc gatcgactcg gagcacgccg aatgggcgcc
      301 caaagcgatg ttcaacctgg gtatgctctt cgaggagcgc ggagagcgcg acctggcagc
      361 gaaagcctac caaagagcgg tcgaatcggg gcactcggac gccgcctcta aggcaaggct
      421 gaatctaggt gtgctcttcg agcagacggg agagtacgat ctggcagagg aagcctacca
      481 acaggcgata gattccggac atcccgaggt tgcgcccgag gggatgcgca atctccgggg
      541 gctgtctacg agattcccca agaagatcag gcggggctag aagcggctcg gcgaaaacac
      601 gctcgcaaga gggccgggtt tgccgaatcg tcgttctcga tcagctcggg cagatcgctc
      661 cgcgagtagt cgagccggtt cgcgttggga atcccgcgca gctggaagga gtagttcatg
      721 atgctcaggt agttgggctt gtagttgaca ttctcatcgc caccatgacg taggcccgtt
      781 gtggcctagc tcatgcatga tcttgcccga ttcttcctgg cgggtgggcc ttccggctcc
      841 cccggacaac tcaacaccgg tactatactt acggggtgac gatatgagcg tgaagggact
      901 ggaagcgcgt ggcctaggat aggccagtga ggatagcttg gtgatcggcg agtcaggagt
      961 acggctcggt aaagcacttc gtcggggaaa gcagcgcgtt gggtgagcgg cgggatggaa
     1021 gaacagacgc gtaacggcgc gccggcccag caggggacct cggcggccgc gtcctccaac
     1081 cttggggtca cgctcttgcg ggtggcctgg ctggcgatcc tgctgggaat ggccatggaa
     1141 ggcatcctgc tcctgctcag cgctggattg ggagatctct tgggtctagg gtccatcgtc
     1201 gctgagttgg ccaggaacgt ttcctggtcc ttattggtct gcctcggtct gtcggtcgga
     1261 aaggccgtcc agagtgcccg cgttccggta atgggtcttc tcggcttcct ggtcgctccc
     1321 gccgcgttcg aaatatcacg cgtgatccat aagggagcga tccaggccct cgcgatctcc
     1381 gggagcgctg gagaggacct ttccccgttc ttgctcgccc tcataaaggg attggagtac
     1441 ggatgcctcg ggctggcgct cggatgggtg ggtcagcgcc cgtggggagg ggcgatggcc
     1501 cacatggcgg tcgggttcgt cgtgggcgcg gtctttggag gaatcgtcat agccctcctg
     1561 gccgcatcgg gccctgaggt gtccgccact attctcttgt ctcggggcgt caccgaggtc
     1621 ctgttcccgg tcggctgctc gctggtcctc ttctcggcgg aagcgttagg gaccagtatg
     1681 gcaagctccg actgatgatc gagcgcatac caaaggcgta aactcgctgt ttgcgcgctg
     1741 aggattcctc cggggttgat cttcttcgtg aggaacctcc tcagccttgc cgccacgact
     1801 acttcgacga acgggcaggc caaccgcgta gtcgggcatc ctcaatggtc gaaaaatagc
     1861 catctggcat atacacggcg ttgctccatt ggttacactg ttaagggcta gggttaggca
     1921 aggtgcggaa agaaaacctt acctgcaatg tttccaacca gagctaatgc acaggccgac
     1981 ccacacaagg gagtaggatt cgtatgagcc gtaatgtgtg tcgatgccaa gcgtggtggc
     2041 tgccatggcg gtgttgaccg tgctgtcagt ggtaaccgtg gcctttgcgg cacaagagga
     2101 aggtggccga ggccaataga agatcaccct ctgccacaaa ggcaagaata cgataacggt
     2161 gggcgcaccc gcgcaagaag cccacctgaa tcacggcgac gaactcgggg catgcggtga
     2221 taccgccacg accaccacaa gcacgacgag caccactaca gatacgacaa cggacactac
     2281 taccaccaca gatacgacaa cgggcactac taccaccacg ggtacggcaa cgggcactac
     2341 tacgggtacc accggcacat gcatcacggg aactactact ggcaccacaa cgggcacgag
     2401 caccacggga acgacggata ctacaggcac cactaccggc accaccggta cgacagatac
     2461 caccactgac acgacgggca ccaccactga cacgacggga acgaccgata cgacgggaac
     2521 gaccgatacc actggaacaa ccacaggcac cacgggtacc accggcgtgt gcaccccggg
     2581 tacgacggac accaccacag gtacgacgac gggaacgacg gatactacag gcaccagcac
     2641 cacgggcact gcggagacca ctggcaccac tggcactacc acgggcgata ccaccacgac
     2701 gggcgacggt ggggatcaag agaaggttac cctgtgtcac gtctccgcga atggcacgga
     2761 gacgatcgag gttgacctct ccgcacaagc ggcccaccta gcacacggag acaccctcgg
     2821 ggcatgcgag cagaccacag gcaccacggg aacgacggat accacaggaa ccacggggac
     2881 taccggcacc acaggcacga ccaccggggg ggagactccc acgccgggcg acggtgggga
     2941 tcgagagaaa gtctgcgttc gtttcaagaa tgatgacaac aagaaataca ggtgggtctc
     3001 cgagggcaag gagcaccgcg gagataaggt agtaaaggac aagttctgcg agcagaagaa
     3061 gaaaggcggg cacaagggga acgaccataa cgcccacgcc gagaagaacg gcgatagcgg
     3121 ccgcggcaac gacgacgatt agccgtagga tcggtcgtag tatcagggca ttctgtcgct
     3181 gaacgcgaaa ccaaagtcgg ccggcgggcc aacccttgtg atcggccgtc ggtagtgcgg
     3241 cgaaggtagc acgcttctct ggaggaaaag gatgcgcgtg gcggtggcat gggcaaagat
     3301 cggggttgca cagccaccac gctgtttttc gagtggcttt gttcaggtag caaagacctt
     3361 gtttgcctgt gtagaatacg cttgaccagc gcaggcatag ggggtggggt ttgcaaagca
     3421 gatttctcat gagcgttgcc gttgctgcgt tcatgatcgc ttatgtcttc gctcctacgg
     3481 ccgcggcgca ggattcgaca gacgaggctg atgacggcgc ggccacagac tcgcaacaaa
     3541 cccagatatt tgccgagggt gaacaggcgg cgcaagtcca agcagacgtg ggtcagccgg
     3601 ggccattggc gggtacggta gtcgaaaccc gtagcacggg tcgggacccg gaagtcgaga
     3661 ttatcattat ccccaggaca gattgcacct tcgagcaacg cgcgtccttc gtcctccaag
     3721 acgacgatgg cacgcaggcc gacttcatcg acaacgtcaa tgtcaagata aaagtggtcg
     3781 acgcgggatt ggaggttgtg agcaacaacc ccggtccaga cacagacata aagcccctca
     3841 acgaaagggg aggggatagt gttctcgata ccggagggct cgtgatcgtc acttccacgg
     3901 gcatcggatg cacagcgagc gctccgctac cgagtaaccc cccgccgagc aaccccccgc
     3961 cgagcaaccc ttcgcctggt agtccgccag cgaacagccc ttcgcctggc agcccgcctg
     4021 cgagtagtcc ttcgaccagt agtccttcga ccagtagtcc ttcggctagt agtccgccgg
     4081 cgagtagtcc ttccactagt agtccaccgg cgagcagtcc gccagcgagc agttcgccaa
     4141 ctggcagcgc atcgccgggg ccagagccgc aggatgtgga gaaagggccc ctggcgggag
     4201 ggacggctat cggcagagat gtgctcgttc cgggagatgt tatagacatt cttcccgatg
     4261 gcacgagggt caacgggata gaccagatca tcatagagac cgaaaactgc gagcttacgg
     4321 cgcagggcag cgacttgacc ataaccatga gcgatcgtgg cgtgcctttc aggatcaggg
     4381 atggtgacaa cgcggacatc acgttggagt cggacggaac catagtcgcc aatggtagga
     4441 gtacgcttgg ggagtcgttc cccgaggccg taagagacaa ccccgacaag ctcatcgtac
     4501 cgatacccgt agacccggag aacgaccagt ttcccagggg ccccaacgac accttcccga
     4561 tattgagctc gacgggcgta ggcggggaag gatgtcgtgt cgtgggtaac agcagagacg
     4621 gcggcggaga tgccggccag gtggaccgcg gtgccagcat agatgacact attacgaacg
     4681 ccggagacct gaactccaaa gacggcgtga tcccggacac gatctcgaat aacgcggtgc
     4741 ccaacacggg cggcatgccg atgctcggtc ccgtcgtttc tgggctcgtg ttcgtcggcg
     4801 tcgggttgct gctcttcagg tcggcggtgc gtaggaacac gtaagccgcg ataggccaga
     4861 cggtgcggaa ggggtccagc cggtgttgcc ggtcgagatc ctgagggagg ccgtagggag
     4921 ctatgcgcct tacctgcaag cctcagggga gatgcacgac ccggtcttcg gtgaacccac
     4981 acagtacggt accccgtatc acgcgttctg caacgccgtg ctggccaggg aagacggcgg
     5041 cgagaggaga tgggcgtacg ctgaccgagc cgtccggggc cttgaggccg ccctcgacca
     5101 cgtcctcgac cctggactcc cgccgaccgc ttccggattc gacggcgcga caggagcggt
     5161 ggaccgatcc aaccacaggg acttcttctg gcccccgatc ttgaagacgt tcctcatact
     5221 cagggagctc ggagtggatg gtgcggacgg cctggcagcc cggatctccg gggtggatat
     5281 cggggccgcg ttcgcccaac ggcccccttc gaactgggcc gtcgtctggc tctcgggaga
     5341 atggctgcgg ttgcgcgaag gcctctctcc gttctcgatg gagaggttgg atctctggct
     5401 ggacacgttc ttcgaaaccc acatcctgtc cgaacaaggc ctctaccagg aaccgggaca
     5461 tcctaactcc tacgacctct tcacccgcta ccacctggca gacatgctcg cggaaggcta
     5521 cgacggccgc cggcgcgagg acctggagat cctcatgcgc acgggcctgc tccgcagtct
     5581 cggcgtgcag ctctccgacg gctccctcgc ctctgcccac cgcagcacgg ggcaaacgtg
     5641 gaccctggga gcccagtgtg cgttcttcac ccacgcatct aaccacttcc gcgggcaaga
     5701 gccacaactc gcgggccgcg ccgaggaagc cgcctacaga gccctctcgt cgttccggcg
     5761 ctggcagcgg gacggtccct acagcccggt cgagaaccgt ctgccacctg gtcacagggt
     5821 cggctacgaa cgctacacgg ccgacggcca ctacgcgaac ctggctgcgg ctttcctcgc
     5881 agcggcggtg tcgaacggct tgaacaggcc cttcacggag cgttccggcg gacgcgaggc
     5941 ttccctcttg ctggagcacg accccaccta cagggcggtc ctccacaacg gcctctacag
     6001 cctgcacttc aacgcccgcc cggcgcccga ctacgatgct ttcggcatcg tggacgtgac
     6061 cttcggtcct gggaggttcc tgcaattcgt ctccagcgcg aagcacctcg aaagcgggcg
     6121 tctcctcaac ctcgggatgg caggcagttc gggatcgtcg caagctgacc ttgccctgac
     6181 cggtaggatg caaggaggac tcgcgccgcc tcgtctggag ctcgaagcga ggaacagagg
     6241 aagtccttac cggtaccgct ttgctgcgcg cgtggagacg gagggggtct acgtcgaaga
     6301 gactacaccc gggctcgaag acccgaagtc gctcctgatc ccgtacctgc gtgatccggg
     6361 cacgggaacc gtcacggcgg cgaaggtgga ggcgggcagg gtacggctgg tccacggacg
     6421 ggaagtggtc gagatctctt acggcgcccc ggtgaagcgc gtcgttcatc tgaccggcgg
     6481 cctacagagc aggcgtggca tctgcggcat gttgaggata gggtttcgag agcggagtca
     6541 gggaatctct taccgcatcc gggcaacccg ttgagtttgc atgctgccgg ctatccactt
     6601 tccggatcga tgcgcaggtg gacggaaccc tccctggccg gttccagaaa cgcgaagaac
     6661 acgcgccgca cgtcggctgg tccctcggcc aggtcaacgt cttcgattgt ctcgacccgc
     6721 agctctaccg cttcggggtc gaagaagacg cgcagggcgc cacgaacccc gcgtagcagc
     6781 accgagtcag gggagatctc cgcctccccg aacgtggtca ggacgctctc gaaggtgcca
     6841 ggacccgtgg cgaacctcac ctcgtctgtc aactctacat ttgcaccagg ttcctctctg
     6901 cgcaagacca ccgtgcgcct gagcgagtcc agatccgcct cgggcgggta gacgtcccgc
     6961 aactcgaacg ccgccaggtc ggcgtggtcg ccggaccggt gttccagtag tcgtgcgccg
     7021 tgttcggtgc caggcagttg ctcctggccg ttgggaacgg gaacggagtg tcccctggag
     7081 gagttggcga tgtgctcgta gcgctcaggg ccgaagtagg ccttcgtata tcggccgcgg
     7141 cctatatcgg agatcacgga ctccccatct acgtgcacga taacgtttcc cacatcgttc
     7201 tggttgtgca tctcaccgtt gtggccgcct ttggtcgcga gcaccagggc gcctgggtct
     7261 tcggggcgct ggcgggagac catccacatc atgccgccga accagtcgtg acgcgcaggg
     7321 acgaaacttc cctcgacgtg ttcgggacgc cacaagaggt cgcgcagggc ccaggtcagc
     7381 tcgcgctcgt ctccgcccgg ctgctcctcg agcagtcgtg cgagatcggg aagttccagc
     7441 ctgcgcgaca ggtaagacag gtgggctggt acgaagccgg cgttcacgtc gcagtcggag
     7501 aagttcacgt agagacccgg gctgagcctg gtgcataaag ggaaccggga gatcctacgc
     7561 aagtgctcgc cctcgaagaa atccaccttc caacccgtgc ggtgtcccac gaggtgggcg
     7621 agcacggtgt agtagccgaa gccgtaagac cagtaggccg gtccttccgt ggagccaccg
     7681 tcctggtcga acgtggagag gtagtcgtcg agagagcggg tggagcgggc caggatctcc
     7741 gccagcctgg ccgcatccgg ctccaggtaa atcgcagcgc ccgccacccc ggcgttgcac
     7801 accgccgtcc agttgttgac cggcctatcc ggggaatcga agagccaggg gtggtcgtgg
     7861 cgctccaggt aagggaccag gcaccgccgg tccacctcgt ggcggatgcg ccttcccagg
     7921 gcgggatcca ggcgatcacc gaggagcagg tctagctccg ccaggtacag cgcggtcatg
     7981 gcgacgctca ggtctatcac cggacggttc atgtcggcca ggtctccctg gtgggccggc
     8041 cacgaccagc tgctctcctc acagatagcc cacgccacgt cgagcagcgg gtccagaaag
     8101 cggccatacc cctcgaggca ctctgcgagg gccaggttcg agagcatctc ccgcctccgg
     8161 ttcgccggct cctcgtatcc ctcccgcgaa ccggcgcgcg agagctccag gtagagcgtg
     8221 gccgttagcc cgggaatctc cgcccttgca tcggtctcgg ccctggtgag gagctccgat
     8281 acccgttgtt cccctacggc ttcgcgtacc ttctcccacg cggcccggtc cgatgccggc
     8341 gggaacggtg gcggcggtga tacctcgttc agcgcgcgtt cgatggacgc gggatcgtgc
     8401 ctgcccagca cggtgtctct agtctccttt accggtccag atcgaagtcg aggtctcata
     8461 cttgaaccag tagttgggtc gtggttggag aagcggaacg tctctgtcgc agtggattcc
     8521 cgctccccgc tttcgcgggg gcaagcttcg cgggaatgac gggagagggg gcgggtcact
     8581 ggtatggtcg agcgtgggtt ggagcctgga tctcaagtcc tctcccaacg ggccaggtcg
     8641 cctccaacgt tccgtccgtc tcgcgcactg ccgggccatg cgccgaagga ggctgatcgg
     8701 tccgcagtaa ctccaggccg gccacgacca ccaccttgcc gagacccgcc gggtcgaaat
     8761 tccggcgggg tccacggtag cacacgccgt ccaggcagac ccgcccttcg gcctgttcca
     8821 tctcccaggc caattcgtgc gggccgaaga ggcctggggt ggtgtggatc accaccctgt
     8881 gagagccggc ggagagctcg aagcggccgt ctccaagctc ctttcccgca acgccctccc
     8941 cgaggagttg gtagcggact cggaaatcct cagcgtggaa ggtgcccgat gccggcacgt
     9001 cgagctggtg gtgggcgtcg cccataccgc tgagcaaacc gaccgcggtg aggatgcgcg
     9061 ggccactctg gccgttgcgg gcgcaagcgg acgagaagtc gcggccgtcg tgcaggaaac
     9121 gcagccgcag cacaaccgcc ggctcctcct ccgtccgcca gtacccgagc accacgcggc
     9181 gctggtccca caggttgtcg cggttgaccg accccaggca ggcgccttcg gacatccagg
     9241 tcgtacccgt ggtggtgccg gcgtctccac cgcggcggat gaacgtactc tgcaactcgg
     9301 tctcctcgct ggcaggcgac cggaagcggt ccaccatgcc agccgggcag ggcagcggcg
     9361 tggtgccttc gaagcggtct ctcaggaccg ctgcggggtg gggacgaatc tcgacgcctg
     9421 tccgatcgga gaggtagccg gcgatcccgg cgtcgagccg gtcgctgtag gcgcggctgt
     9481 ggggaccggc ccattggctg gtaccgggat ggtaatgttc tgcgatggtc tgccaggccg
     9541 cgccccgcaa cctctcggcg tcggcgcggg ctgaggggtc cctcaccagt tgcagcatcc
     9601 gctcgcactc ggccagggtc acgacggtgt aggtaggact gttgtactcg ttgaaaccgc
     9661 cgtggtgctc ggtgtgctcg actatgttgc gcaggcgacg gcgaccgtag tcagcgaaac
     9721 ggggttcatc gagcaattcc ccggcggcga gcgacacgcc ggcgcccata acggcgatgt
     9781 tggtgtatgc gggtccgacg ttgcggcgga agatcgcggc cgcagcgtgg cccaggcccg
     9841 tctgtgtggc tccgaccagg ctctcgggca gatccgcggc gtgctcgacg agcagttggg
     9901 ccagcctggc gccgcagaag tctgcccagt tccagtcggg cggatccatc ccggtgagcg
     9961 gttcttccag gagccacggc cagatgccgt aggtcttgct cgttgggtcg gtatcctgga
    10021 gtgagagtac tctcgatact atggccgccg cccgtttctt tccgcccgac cgtaggagcg
    10081 tcagggcgta gtccagcgac tcgcgcgtcg ggtgcaccca ggcgccctgt gggacgcgcg
    10141 tgtggtagtc aggacctttc caggagagac caagcatgct gtgtacatcg ctgtagacag
    10201 cgccgttcag gagtccctct cccttgggca tcggcgccta ccttgcctgg tggggctggt
    10261 gtctggctcg tctatcgcac ggccccctgg tcgcccacgc ccctgagatc caggtcctcc
    10321 gcgaagtggc aggcggcgta gcggccctgg gttaactcgc gcaatggtgg ctcctcgtcc
    10381 ttgcagcggt cttcggcgta gggacagcgg gtgtggaagt agcagcccga gggcgggttt
    10441 gcggggtcag gcacgtctcc acctagtacg atgcgccgtc cacggtctct gaggcgcggg
    10501 tctggcttgg gtacggcaga gaggagggct tcggtatagg ggtgtttggg ctcgtcgaag
    10561 atctccccag tctctgccac ctcgaccacc ttcccgacgt acatcacagc cacccggtcg
    10621 cagatgtgct cgacgacgga gaggtcgtgg gagatgaaga ggtaggtgag gtcgaactcc
    10681 tcctgcaggt cttgaaggag gttgaggatc tgggcccgca ccgagacgtc gagggcgctc
    10741 acggcctcgt ctgccaccac gaagcggggg tcgagggcca gggcgcgggc tatgctgacg
    10801 cgttggcgct cgccgccgga gaaggcgtga ggatagcggg acatgtactc cggcctcagg
    10861 ccaacgcggc gcaggagctc ggccaccctg tcctcgagct ccttccccga ggcgaccttg
    10921 ttgaccttca ggggctcgcc cactatctgg agcagcgtca tgcgcgggtt gagggacgag
    10981 ttggggtctt gaaagaccat ccggatctcc ctgcgatatg gcttgagcgc agtgctgccg
    11041 agcttcgcga ggtccgccgt gcccccgtct ctattgtggt agaccatctc gccctcggtg
    11101 ggctcgtatg accttacgat acagcggccc agggtggtct tgccgcagcc gctctccccg
    11161 acgagcccga gggtctcccc gcgccggatc ttcagatcta cgtcgtccac cgccttgacg
    11221 tgccccactg tccggccgaa gaagccctgc tttatgggga agtgcatctt gaggtgcttg
    11281 acctcgagca gggcgtcgcc tccagccccg ttgcgctccg cgccttcagc ctccatcctt
    11341 gctcccctcc tttccgccgt agagcagaca ccggacctcc ctggtgtcct tcagaggctg
    11401 gtcggaagcc ggactcgggt catgttcgtt gccgacgggc tcgcccgagg aggaaagggg
    11461 tattcgcggc ggcagtatct cttcgcagta gccctccatg atctcgtcgc atcgggtgtg
    11521 gaaggggcag cccgtggggc ggttgtaagg atggggaacc atgccacgga tagagtcgag
    11581 cctctgattc gccttgttgc ggcccatcct cggaatggag cgcaagagcg ccctggtgta
    11641 gggatgtttg ggatcgtgga agatggagtc gacgtccccg cgctcggcga ccatgccaag
    11701 gtacatcacg gccacatcgt cggccatctc ggcgaccact ccgaggtcgt gggtgatgaa
    11761 catgatcgcc atgcccagct catcctgcag cccgcgcatt agctccagga tctgggcctg
    11821 ggtcgtcacg tccagggccg tggtcggctc gtcggcgatc agcaggctag ggtcgcagga
    11881 gagggccatc gcgatcatgg ccctctggcg catgcccccc gagagctgga aggggtaggc
    11941 gtccaccctc tggcctggat tggggatgcc gacgcgggcg agttcctcca ccgtacgctc
    12001 tcgggcctcc cccttgtcca tcggcaggtg gagcaggatg ttctccatta tctgctcgcc
    12061 gatggtgtgc acgggactca gcgacgtcat cggctcctgg aagatcatcg agatctcctt
    12121 gccgcggatg tcccgcatgg tcttgctcct ggggtccaac gacgcgaggt cgaggacctc
    12181 ccgcacttcg gtcgccgtcg ctgacccttc cttccgagct tcgaccttac cgttggactc
    12241 agtccgcgga gtggggctga agaggatctc gccctccacg atacggcctg ggcggtccac
    12301 gagctgcaag atggagcggg ccgtgacgct cttaccgcag ccgctctccc cgaccacgca
    12361 cagggtctct ttgcgtttta ccctgaggtc tacgccgtcg acggccttta cgaccccctc
    12421 gtcgaggaag aagtgggtct taagtcccct gacttccagt agagagtcgt ttgagtccat
    12481 gctcttatct ggcatagggg tctgccgagt ctctcaggcc gtcgcccatg aagttcagcg
    12541 ccaggaccgc gatgatcacg gcgaacccgg gcagcaagag ccacggcgcg gtggcgacgg
    12601 atcttatgtt ctgggcttct tgcaggagca caccccaact cactatcggg ggctgcaggc
    12661 cgagccccaa aaacgagagg gccgtctcgg cgaggatcat ggccggaacg gcaagggtca
    12721 gggacgcgat gatgtggcta gcgaaagagg ggagcatgtg ccgcaggatg atgcgaggct
    12781 tgctggagcc gtcgagctcg gccgccgtga cgaagtcctc cccgcgcaag gagaggaagc
    12841 gtcccctgac gacccttgcc aggtccgtcc agcctataag ggagaggatg atcgttatgc
    12901 cgaagtagat cctcaagggg ctccaatcgg gtggcagcgc ggccgagagc ccgagccaca
    12961 aaggcagggt tggtatggac atcagcacct cgatcagacg ctgtatcgca gtatctgtcg
    13021 ctcccgcgta gtagcccgag atgccgccca aaaagacgcc gaaaacgaag ctaagggcca
    13081 cccctacaag gccgatggac atcgagacgc gggcgccgaa gatgatgcgg ctcagcatgt
    13141 cctgcccctg gtcgttggcc cctaggaggt acatcggctg tcccttctcc acgggtccga
    13201 tcaggtggcg gtcggactcg aacaggcccc acagcttgta cgtgtttccg tgggcgaaga
    13261 gcctcacggg caccttcttg gactcgtcga cctggaacgt gcgcacgagc gtctcttcgt
    13321 tcaacttcga gctgtaaccg tagacgtaga gcccgaaccc gtcctcatcg aagaagtgca
    13381 gacgctgcgg aggggcgtac gtgtagtcgg cgtcgaaggc atcgggggtg aagggggcca
    13441 cgaactcggc gaagagggcg atcaggtaga tcaggatcac caccaccgtg ccgactacgg
    13501 ccagcctgtg cttccggaag cgccaccaca ccagcttcca ctgggaggcc acatagattt
    13561 cggcttcctt ctctccgagg tcggtcgccc gctgccgggc ttcctgctct tcttgaactt
    13621 gcggttgcgg cggctccgtc aacgctcctc cttattggta ctggtaacgg atgcggggat
    13681 ccaaccaggc cagcaggatg tcggatatca gggtccctat gaccgtaagc acgcttatca
    13741 tgaggatgat gctgccggcc aggtacatat cctggctgag tagcgcccgc aagagcatcg
    13801 ggcccgtggt ctgcaggccc aagacttgcg cgactatcac ttctccggag aggagcagcg
    13861 gcaacaccca cccgacagtg cttacgaaag ggttcagggc gacacgcacg gggtacttga
    13921 cgagcagcct ccgctcgggg actcccttgg atcgggcggt cgtaacgtag ggcttgtgca
    13981 gctcgtcgag caggttcgcc cgcacgatgc ggatc
//
LOCUS       DATMVF010000276         5737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_546427, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000276 DATMVF010000000
VERSION     DATMVF010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546427"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VN178_14380"
     CDS             complement(<1..206)
                     /locus_tag="VN178_14380"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HWS82203.1"
                     /translation="MAQQIRVDDFVGSSSESWSEAARDAVSQMSGMAESIQGVDVLSQ
                     SAEVENQEITEYRTTVRVAYTPSS"
     gene            510..1094
                     /locus_tag="VN178_14385"
     CDS             510..1094
                     /locus_tag="VN178_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS82204.1"
                     /translation="MTPFEGNDRLLSVEDAARRLEVSPITVQRWCGDGSLPCLSIGGL
                     WRIRSPALEGFLSPGERDEPLAERLRGFLEVPDNVLGIAQDEELIRKLDAAFFLVGDA
                     RGGMLVKYYNEELDSAEGLRSDFEREGLEVSRLEGEGRLRIVAQGTLSGGDRSGELRR
                     LAGEEAPEGRSVWANFNWNDQVDLDTALSQQEEL"
     gene            complement(1453..1746)
                     /locus_tag="VN178_14390"
     CDS             complement(1453..1746)
                     /locus_tag="VN178_14390"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWS82205.1"
                     /translation="MPTYVVLMNWTDQGVRTVTDTVHRREQADALAEKHGARIEQVYW
                     TVGPYDIVTIFEAPDDQSATAMLLELGSGGNLRTTTLRAYDREEMAGILQRLG"
     gene            1878..2150
                     /locus_tag="VN178_14395"
     CDS             1878..2150
                     /locus_tag="VN178_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82206.1"
                     /translation="MNGEYLRTAAARSRGQGSGHDQPGAAGPRRERLEDLERDKEALL
                     EYHARLVPEDLDGLTSEQRQTLYRMMRLKFWPATECLPLPSGVVMP"
     gene            complement(2653..3243)
                     /locus_tag="VN178_14400"
     CDS             complement(2653..3243)
                     /locus_tag="VN178_14400"
                     /inference="COORDINATES: protein motif:HMM:NF023134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWS82207.1"
                     /translation="MKESRWNSEANWNTPAPGELELVRRFLNTWRIDADTREPADELP
                     WLLKDRRAWEERFPTRPPGDPDGDAGELLVRLREDLRGALGAPEGWTEALNGWLEGYP
                     LVARVVEENRGASVRYEPGPEAGLAGRVLAAVARSVEDGTFFRLKACPDCRWVFYDKS
                     RSKTRVWCGMYAGEGGRACGTISKVRRYRQRHKAGE"
     gene            3389..4318
                     /locus_tag="VN178_14405"
     CDS             3389..4318
                     /locus_tag="VN178_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS82208.1"
                     /translation="MGSRQDITLWIAVAAAVFFSASGFTGTEVGLEGYSPGPLALLRF
                     LVASFVLVLYSALWRMRLPKARDLPALALAGFLAFSAFTVLLAYGQLTVPAGTASLLI
                     ATIPAFTALCAVAFLKERIGSVGWAGVAVSFLGVAMISFGEGEGFGIDPGALLVLLAA
                     LSTSAYFVLSKPYLRGYGAFEFTAYAVWAGTLFLLPFSGRLTEEALQAPLGSTLAAVY
                     LGVFATIAAYASISYAFSRLPASRAVTLESLIPPAAILIAYLWLGEVPSVLSLVGGAL
                     AILGVVLVNTRGEKAEKKGPSRLPLPQSPYETE"
     gene            4467..5084
                     /locus_tag="VN178_14410"
     CDS             4467..5084
                     /locus_tag="VN178_14410"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWS82209.1"
                     /translation="MCLATVHRRSWCSLYAALSKGRIDAEALRALLVSHGPGKAEAPV
                     YAVDVSPWPRCDAEASPGRGYLYHPSRHSAGRPIVAGWAYQLVARMGFERDSWVTPVD
                     ARRVEPEEDANRVAAEQVRALLYRLPDRDDAPLFVFDAGYDPVRLQQGLEGCCVQILV
                     RLNSGRVFYFDPEPLAQPLTNYLKRDRRYWRRYYSKMQATLMSPR"
     gene            complement(5045..>5737)
                     /locus_tag="VN178_14415"
     CDS             complement(5045..>5737)
                     /locus_tag="VN178_14415"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWS82210.1"
                     /translation="RADRRGVLDAVIFRLRTGCQWNRMPKELPEDATVYRTFQRWVDI
                     GVLDRIWATLVEECEELGGVDWEVAGSGRGDGQGPFWGDLIGANRTDRGKNGVKRSLL
                     VEADGGPLSVVVTGANVVRDDKLLEATIDAIVVERPRPTEEAPQHLCLDKGYDNRPTR
                     KIVEERSYVAHIRCIGEEELDEAGEKRYPARRWVVERTLGWLSKCRALLVRYDKKAAN
                     YLGLIKVACILL"
BASE COUNT          941 a         1875 c         1795 g         1126 t
ORIGIN      
        1 tagctgctgg gggtgtaagc cacccgcacc gtggtgcggt actcggttat ctcctggttc
       61 tcaacctcgg cgctctggga gaggacgtcc accccctgta tcgactcggc catccccgac
      121 atctgcgaga cggcatccct cgccgcctca gaccagctct cgctggagct cccgacgaag
      181 tcgtccaccc tgatctgctg ggccatcttg cttcctcctt ttctctcctg ccatgccaat
      241 gtacccccga gttgctaaaa acgtgtccct acttgcgaat ctcttgggca aaacgtcccg
      301 aagcgcgaag aagggtgcct agctgatagc ggctctggcc ctcttgcctt tgccccgcac
      361 cctccgggta tccgagaagg gtttgctggc cctggtatga tggccacctc acaaagggac
      421 gggtttggtt ggtactagat cgcctctcgc taaaaacgag aggtcttttg ctttcggtaa
      481 gcccccgagg gaaaggaggg cttgatcgta tgaccccctt cgaaggtaac gatcgactgc
      541 tgagcgtcga agacgccgca cggcggctag aggtgagccc catcacggtc cagcgctggt
      601 gcggcgatgg gagcctgccg tgcctgagca tcggcgggct gtggcgcata cgcagcccgg
      661 cactcgaggg ctttttgagc cccggcgagc gcgacgagcc gctggccgag aggctgcgcg
      721 ggttcctgga ggtgcccgac aacgtgctgg ggatcgccca ggacgaggag cttatacgca
      781 aactggacgc cgccttcttc ctggttggcg atgcgagggg cgggatgctc gtcaagtact
      841 ataacgagga gctggattcg gcagaggggc tgcgttcgga cttcgagcgc gaagggctcg
      901 aggtgtcgcg cctggagggg gaggggcgat tgcggatcgt agcccagggc accctcagcg
      961 gaggcgaccg gagcggcgag ctgaggcgtc tggcgggcga ggaggccccc gagggccgct
     1021 cggtgtgggc caacttcaac tggaacgatc aggtagacct ggacacggcc ctaagccaac
     1081 aggaggagct atagaagctg gtcgagggta cttcgctggt ggtccagacg gtggtcttag
     1141 agcgggcgct cgacgagtgg ccggggggat gttgcgggaa gcgcaggtca agcactcggc
     1201 cacgctgtgg ctcaccgagt cggggttagc ccaaacccgc gtggtgccga ctccttcggg
     1261 tagcgaggcc ttctagcggt ccgaagaacg ttacgggggc caaagcccag tttccgagaa
     1321 gactttagta ccggcaaccc ggtttccggg aaggtccctt ctcggcatct aggtgaatta
     1381 gggcaaagga agaagggcaa aggaagagcc gggcttcata gaagaaaccc ccggccccgt
     1441 gacgagggga gctcagccga gtctttggag gatccccgcc atctcttcgc ggtcgtaggc
     1501 gcgcagcgtc gtagtcctca ggttgccccc cgagccaagc tcgagcaaca tcgcggtggc
     1561 gctttgatcg tcgggggctt cgaatatggt cacgatgtcg tacggaccca cggtccagta
     1621 gacctgctct attcgcgccc catgcttttc ggccaatgcg tcggcttgct cgcgtcgatg
     1681 cacggtgtcc gttacggtcc ttaccccttg atcggtccag ttcatcaaga cgacgtaggt
     1741 aggcatacct acttgtcctc cttcctcccg cttttcaggc ggtctcgacc tttccactac
     1801 cggtatgatg ccacccaccg gagaccggcg taacccccaa ttaagtgttt tgggtgtgag
     1861 aacgcgaagg gcgggcggtg aacggcgaat acttgaggac ggcggcggca cgctcgcgag
     1921 gccaaggaag cggccacgac caacctgggg ccgcgggccc ccgtcgggag cgcctcgaag
     1981 acctcgagcg cgacaaggag gccctgctgg aataccacgc tcgcctcgtt cccgaggatc
     2041 tcgacggtct cacctccgaa cagaggcaga ccctctaccg gatgatgcgc ctcaagttct
     2101 ggcccgcgac ggagtgctta ccactgccga gtggggttgt aatgccttaa cgacacctcc
     2161 tggtagtttc gcggttacta cgagcgtctt cgggctccgc gccctgctca ccggggaagg
     2221 cgccgagctg gagttgacga gggtctgacc cgtgattaat actccacgca caaagcctcc
     2281 gaagggtaca aggagcaggt cggaatctgg cttgggtctc gtacagaaat ctgctgatgc
     2341 ctacaaatct attctcgact ccatcttttt tactacggcg agagtgccgg cggtggtaaa
     2401 ggttaggggc atacccttct gagagccgtg cgcccacgag caggaggcgt gcttcgccat
     2461 aggcgagctt acagagaggc cccggagggc ttgcgcgagg aaccgcttgt cgagctactg
     2521 aatcggatgc ggcgtacaac acggcatcgc aaggtccacg ccaatgaacc cgatgcggcc
     2581 taagcttcgg atttaactat tcgggctggg ttcggcgacc cgcagagtct cagcgctctc
     2641 gtccgatcac gcctactcgc ccgccttgtg cctttgccgg tagcgcctga ccttggagat
     2701 cgtgccgcac gccctgcctc cctccccggc gtacatcccg caccacaccc gcgtcttgct
     2761 gcggctcttg tcgtaaaaga cccaccggca gtctgggcaa gccttgagcc taaagaacgt
     2821 gccatcctcg acgctccttg ccacggcggc caggacccgg cccgcgaggc ccgcctccgg
     2881 cccgggctcg tatcggaccg atgcccctct gttctcctcg accacccggg ccaccagcgg
     2941 gtacccttcg agccacccgt tgagcgcctc cgtccagcct tccggggcgc ctaaagcccc
     3001 ccgcaagtct tcacgcaacc tcaccaggag ttccccggcg tccccgtcgg ggtcccccgg
     3061 cggcctagtc gggaacctct cttcccaggc ccggcgatcc ttgagcagcc agggcagctc
     3121 gtcggccggc tcgcgcgtgt cggcgtcgat cctccaagtg ttcaaaaagc gccgcacgag
     3181 ctccaactcg cccggcgccg gcgtgttcca gttggcctcg ctgttccaac ggctctcttt
     3241 catcttgttt accacctttt actaaaagac ggttgcgtca tcgttctcct tagtttacca
     3301 ttctcgtatg tttgccggtt aacaaaccgc gccgctccgg cctaggggtg aggcagcttc
     3361 gttaggagag aagggaggcg cacaaccttt gggttcgcgc caggacatta cgctttggat
     3421 cgccgtggcg gcggccgtgt tcttctcggc ctcggggttc accggcaccg aggtcgggct
     3481 cgagggctac tctcccggtc ccctcgccct gctgcggttc ctggtcgcct ccttcgtact
     3541 cgtcctctac tccgcgctct ggcggatgcg cctgccgaag gcgagggacc tcccggcgct
     3601 ggccctggcg ggcttcctag cgttcagcgc cttcaccgta ctcttggcct acggtcagct
     3661 taccgtcccg gccggtacgg cgagcttgct catagccacc atcccggcct tcacggccct
     3721 ttgcgcggtg gcgttcctta aggagaggat cgggtccgtg ggctgggcag gggtggcggt
     3781 cagtttcctg ggggtcgcca tgatctcctt cggagaggga gagggcttcg ggatcgaccc
     3841 gggggcgctc ttggtgttgc tcgccgccct ctcgaccagc gcctacttcg tattgtcgaa
     3901 gccgtaccta agggggtacg gggccttcga gttcaccgcc tacgccgtct gggccgggac
     3961 gcttttcctg ctgcccttct cgggcaggct cacggaggag gcgctgcagg ctccgctggg
     4021 gtctactctg gcggcggtct acctgggcgt gttcgccacc atcgcggcct acgcgagcat
     4081 ctcctatgcc ttctccaggc tcccggcgtc gagggcggtt accctggagt ccctaatccc
     4141 gccagcggcg atcctcattg cctatctctg gctcggggag gtgccttccg tcctgtccct
     4201 ggtgggcggg gcgttggcga tcctcggcgt ggtcctggtc aacacgcggg gcgagaaggc
     4261 ggaaaagaag gggccatcga ggctcccctt gccacagtcc ccctacgaaa ccgaatgagc
     4321 tggtggagcc gaaccgggac tgcccccgac gacagaattc gggcgttccg cacgcagctt
     4381 cacgggtgct tcggtcgtag ggcggcgccc tcttcgagct gacggactcg atcctgagcg
     4441 cagggcgtgt cccatccgcg gtccacctgt gccttgcgac ggtccaccgt cgaagttggt
     4501 gcagcctcta cgccgcgctc tccaaaggcc ggatcgacgc cgaagcgctc agggcgttgc
     4561 tggtttctca cggtcccggc aaagcagaag cgccggtgta cgcggtggac gtttcgccct
     4621 ggccgcgctg cgacgctgaa gcgagccccg ggcgaggcta cctctaccat ccgtccagac
     4681 actcggcggg ccggcccatc gtcgccggat gggcctacca gctcgtggcg cgtatgggct
     4741 tcgagcgtga ctcgtgggtg acgccggtgg atgctcgcag ggtcgagccg gaagaagatg
     4801 ccaaccgcgt ggccgcggag caggtgagag cacttctgta ccggttgccg gaccgcgacg
     4861 atgcacccct gttcgtcttc gacgcgggct atgatccggt gcgactgcag cagggcttgg
     4921 aaggatgctg cgtccagatc ctcgtgaggc tgaactcggg gcgcgtcttc tacttcgatc
     4981 ccgaaccact ggcgcagcct ctaactaact atctcaaaag agacagacgg tactggcgac
     5041 gatactatag caagatgcag gctactttga tgagtcccag gtagttggca gccttcttat
     5101 cgtagcgtac cagcagcgcc ctgcacttcg atagccaccc cagggtgcgt tccaccaccc
     5161 aacgccttgc cgggtagcgc ttctcgcctg cctcgtcgag ctcctcttcc ccgatgcacc
     5221 gaatgtgggc gacgtagctt cgctcctcca cgatcttcct ggtcgggcgg ttgtcgtagc
     5281 ccttgtctag acaaaggtgc tggggcgctt cctccgtggg tcgcgggcgt tccaccacta
     5341 tcgcgtcgat cgtagcctcc aagagcttat cgtcgcgtac tacgttcgcc ccggtgacta
     5401 ccaccgaaag cggtccgccg tcggcctcga ccaagagaga acgcttcacc ccgtttttgc
     5461 cgcgatcggt gcggttggca cctatgagat ccccccaaaa cgggccttgc ccatcgcccc
     5521 gtccgctgcc tgccacttcc cagtccactc cgcccagttc ctcgcactcc tccaccaggg
     5581 tcgcccagat ccggtccaag acgccgatgt ccacccaccg ttggaaggtg cggtacacgg
     5641 tcgcatcttc ggggagctcc ttgggcatcc ggttccactg gcagccggtg cggagcctga
     5701 agatgacggc gtccaagacg cctcgtcgat ccgctcg
//
LOCUS       DATMVF010000277        18517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_636438, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000277 DATMVF010000000
VERSION     DATMVF010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18517
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636438"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VN178_14420"
     CDS             <1..412
                     /locus_tag="VN178_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS82211.1"
                     /translation="AYSLFEIASPPGGGPPPHIQHREDESYYLLEGEYRFLVEGRAVR
                     INAGSLIYVPRGTLHAHKNVGEGTGRLLLSQTPGGSHERFFEEIGEPAVDGSRPPVQA
                     DQQHMEKIIAIATDYGIEIPSPVRSDGGQQRIPQ"
     gene            481..828
                     /locus_tag="VN178_14425"
     CDS             481..828
                     /locus_tag="VN178_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82212.1"
                     /translation="MFARMFTIEGRREQLDEFSRLGENKVLPALQRLHGFEGSLVLAN
                     RQNGHILIVPFWQSEETMRRAEDASHWFRGFVAEAAGGEVTNVERYEVVHPETEGNAA
                     SSRAWDLRVPAGK"
     gene            complement(893..1039)
                     /locus_tag="VN178_14430"
     CDS             complement(893..1039)
                     /locus_tag="VN178_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82213.1"
                     /translation="MSSAASRNLDQRAMPRASFQRAIIAAGGAGNEDRLSGRNTTRFG
                     ERFE"
     regulatory      1063..1146
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            1353..3857
                     /locus_tag="VN178_14435"
     CDS             1353..3857
                     /locus_tag="VN178_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWS82214.1"
                     /translation="MRRLFAPRQLGDALKKSEARYRTVLDAAFDAVVTITTDGVVRWF
                     NRGAEAIFGYRADEVIGQPVMLLMPERYRDLCEAGLQHYLRTGEARVVGGTTELVGLR
                     KDGSEFPIEMSLGETNEHGERLFTGMFRDVTERKRTEDALREARDRFRGIFENAPIGV
                     AMVTLEGRYLQVNHSLCRILGYTEEELQTLTWQEITHPDDLAASQAYARRIVAGEFPR
                     YHLEKRFLHADGHTVWASLSVSLVRDSEGEPLYFVSQIQDVTERKEVERAIVEREERF
                     RSLVQYSSDIITILDADGTVRYASPAVEKVMGYKPEEQIGTNAFGTVHPDDRDRALNT
                     FAEVLKRPGLHPPLEFRVPHKDGSWCYLEHVVNNLLEDPAVRGVVINSRDVTERNAMQ
                     KQLHYQAFHDPLTGLPNRALFMDRLEHALTRANRRGSKIAVLFMDLDNFKVINDSLGH
                     KAGDQLLIEVADRLKAPLRPEDTVARLGGDEFTILVEDISGLGDVARIAVRLAQKLRP
                     PFALGEQEVYATVSTGIALNSPAREQPADLLRHADLAMYRAKHRGKARYEVFEPSMDG
                     EAVERLILETGLRRALVREQFRVYYQPIVALENDRIAGAEALLRWEHPQRGLLLPEEF
                     ISIAEETGLIVRIGQWVLREAGKQARIWQERYPGTLPLTISVNLSTREFFHPKLVAEV
                     LNESEIDPASLQLEITEGALRTNGTYSTDRTLRNLKRMGVQLAIDDFGLGYSSLSYLK
                     GFPVDFLKIDRSFIAGLGGAPNGVSTKDTEIVKAMIELTHALGLKVIAEGVENAEQLA
                     RLRQMKCDLAQGNYFSEPLPSEALAVNLERNLTDRG"
     gene            4109..5542
                     /locus_tag="VN178_14440"
     CDS             4109..5542
                     /locus_tag="VN178_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HWS82215.1"
                     /translation="MDNQLLDLVVLALYFAVMIGAGYWGLRRARSADDYLVAGRRLGP
                     FMYIGCLSAVVLGGASTIGGVALGYDNGISGMWLVFWIGMGVIALGVLMSSRLSRLGV
                     YTVSEMLENRFGAASRLISAIIIAAYALMIAVTSTIAIGTVFNVVLPLSSSVAILVAG
                     GLVVVYSVAGGMWSITLTDIIQFCIMTVGIFFLLLPISIFAAGGLSGMREALPASYFD
                     VTAIGWQTIFTYFLLFFFGLMIGQDIWQRVFTARSPEVARWGSVAAGVYCLLYALAGA
                     LVGTAARVILPDLASSDNAFARITTEALPAGITGLVLAAALAAVMSTASAGLLASSTI
                     LANDVYAGFIARGDHNKVLVSRATTLLVGIVVLVISLIVSDVVGALTVAYDLLSGALF
                     VPIVGALFWRRATAAGALSSMAAGSVVVVIFMVLDGLFANTPIIWGMLASLVAFVVVS
                     LLTPRPSEERMRTWERRLTGSGAEGEI"
     gene            complement(5676..8045)
                     /locus_tag="VN178_14445"
     CDS             complement(5676..8045)
                     /locus_tag="VN178_14445"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS82216.1"
                     /translation="MTGQRLVMGAEQRSSFGAKLRRLRESAGLTQEELAARAGLTRNA
                     VSALERGERRRPYPHTVRSLAEALDLSEAERAALVAAVPGREDGQAGFLRPATLPAPT
                     PLLGRERDLREVLDLLHRPEGRILTLTGVGGVGKTSLAVRAAHDSAEHFGHGVAFAAL
                     APLGDPSLVLPGVVRTLGLTEPRGATSRDVLVSYLRDKRMLLTLDNFEHVLEAAAEVA
                     YLLEACPHLAIMATSRAPLRVRGEQEYPVEPLALPTSTRSPDPGKVLGSPSGRLFVER
                     AKAIVPAFTLGEEKAAAVASICWRLGGLPLAIELAAARTRILSPSSLLARLDQALSAG
                     WAQDLPERQRTMHATLDWSYELLSEMERALFRHLSVFTGGFTLEAAEAVETTAGVATE
                     DVLGLLGRLVEQSLVTAKTGGSEERYGMLEPVRQYALEKLEESGEAGEMRRRHAAFFL
                     DLAEEARPGLKGSRQVEWLEKLEQENGNLRATMSWALTEDEVETATRLGWALWTFWWL
                     RDHQQESHRWIERLLELEIPPVVRPRAFQIAAMTAYLQGDRERSTGLLAAGLELSKRA
                     GDTLCEAYVRFWLGLEAVDREDFEKAISRFEEALPLFRESGEEEMVSAVYDRLGMVAL
                     RQDDLDRALTMLERALAQSRKRGDRLGTYSALYFLAQIALARGDYAAATRMFEEGVFL
                     AAQVGLRGGLAYLLEGLAAAAEAGGEAERSARLFGAAEGLLQMVEAPLYRSHRPRRSL
                     RPLEHTMVAVRSRLGEEAFEEARAAGRAMAFDAAVEYALGTYNSGVQKG"
     gene            8227..8790
                     /locus_tag="VN178_14450"
     CDS             8227..8790
                     /locus_tag="VN178_14450"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS82217.1"
                     /translation="MVPEEIAGSGRRSAHAAPEQDRRSLWVLGELVTCKVASAQTSGA
                     YSLFEVTSPPGSGPPAHVQHRENEAFYVLEGEYEFLVEDRTVRVSAGCLLHVPKGTLH
                     RHDNVGDMPARMLVIQTPGGLHQAFFEEIGEQTSAKRESHVPERPESLARTREIAAGY
                     GIEILPAEKQNREMSRRQNDGGRSLHE"
     gene            8910..10154
                     /locus_tag="VN178_14455"
     CDS             8910..10154
                     /locus_tag="VN178_14455"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWS82218.1"
                     /translation="MAKERTGQRHKNKIYFEDQDLDLYLQAFPLNHLGYGGAAAGEVF
                     YAASRINEKDLESWVAEWSMLATRLEAQAAKALQRNHIVSARESYLRAFTYRRTATLG
                     LRATDPRFRAEWETMRSCFRRAAKLFEPPIETVEIPYGDAVLPAYFVRAGDQRRPTVI
                     VIGGGEQFCEELYFWGGAAAVERGYNALLVDMPGQGGTPFTGMHHRHDVEVPMSAVLD
                     YLQDRTDVDAGRVGAMGFSLGGYIVLRSVAHDKRIGACAVSTPIIDWHQTLMDALPTV
                     LRRAPRPLFNAVMKLGNFFGKTQLLAYEKFFEWQVGAASYTEAFDSFRPWKVDIQSIS
                     CPVLCMMGTGEAATFKKQTQACYAALRSPKTLRVFTEEEGADAHSQANNQRLAHQTIF
                     DFFDETFARDEANRELGLAQGA"
     gene            10216..11469
                     /locus_tag="VN178_14460"
     CDS             10216..11469
                     /locus_tag="VN178_14460"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWS82219.1"
                     /translation="MARTTMFQRSRTKVRFSNEDMDYLFQVVRGYHTHGGASFGELFH
                     AAAKVEEGDPESWISAFREMGERIERQAEELEGRGKASAALAFLRAFTGYRAAAFLMN
                     PKADIERFTETVESFVRCFRKAIPGFGNPCEPIRIPFGGSYLSGYFYQVNEQTVPRPT
                     VIVVGGGDTYAEDLHFWAGAAGPTHGYNVLSIELPGYGLSPTRGLHLFAGLEAPMAAA
                     VDYALSRPEVDAERLAVYGISGGGYAVTRAAATEERIRATVATTPIYDIYRLITEAIP
                     NFLLDESHSGVSRWLLEISGRFNKAGKNNMDKFAWQSGKGSLLEAVRVTEGEPVDVGA
                     IQCPMLCLAAEGDPPEALGQTKFVFDHLEHPKKAMRVLTAEEGAEAHTHVNNFPLLHQ
                     VVFDWLDETLTPKDITQRVRLADGA"
     gene            11634..12179
                     /locus_tag="VN178_14465"
     CDS             11634..12179
                     /locus_tag="VN178_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS82220.1"
                     /translation="MRATVYIATSVDGFIAREDGGIDWLPDEGDEEGGEDYGYQEFID
                     SVDAIVMGRSTYELALSFGSWPYGEKPVTVLSSRHVEIPDAIARTVEAMSVAPREVVQ
                     RLAERGYGHLYVDGGRTIQGFLRDGLIHQLILTKIPILIGTGIPLFGPLSHDIGLRHL
                     ETRQFENGLVQSRYEVLGNVT"
     gene            12744..15047
                     /locus_tag="VN178_14470"
     CDS             12744..15047
                     /locus_tag="VN178_14470"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWS82221.1"
                     /translation="MILLFNGLLVALVLFKPISDVTLALVVNAAQFIGPLLALPLCFG
                     GLLRRVWERGASQAHVGTAVRRGQRWAPVLLGLGILSWVFGQMVFTYYEWVLGRPPPL
                     PSIADIGYLSVYPFLLLGILFLPSRPIPVASRTRIALDGLMSMTAAVTFSWYFILGPI
                     MQQGSETILAKAVSSAYPLAGIFLIACLIILASRPEELTLLPAVRILALGLTLIVIAD
                     SNFAYWSLHDAYATGTLPDIGWSLGYMLVALGAFAARLAPGEEVTMTPGEYVDTSGTA
                     SPLPEQRVWTALMPYVLVPAVGILVVYAWRQSAGSGSLATGVYLGGALLIGLMVLRQV
                     LTIVENARLYNRLQRTHLQVEQKNDQLVRSQSELRRQKEYFESLVLNSPVAIAIVDLG
                     GNVVSWNPAAERLFGFTQAQAIGRSIDDLLAGTPQMHAEVLEYTRQTASARRVDVVTR
                     RSRKDGTLVDVELLAVPVNVGGDHVGTYAMYHDITELKRVEEEVRQLNRDLEKRVAER
                     TEQLKSAMAREQEVAKERERIEQELRVARMIQHTLLPKSLPELEGYKIAVHYQPAREV
                     GGDFYDLLQLPDGRLGLIIGDVSGKGVPAAIVMAITRTMLRSAYHLGSPGEILQRVND
                     ELFPDIPPNVFVTCLAALLDSKTGRLQYANAGHDPPFVRHASGVGKLRATGMPLGLMA
                     GMVYEQKEITLEPGEGVLLYSDGLAEAHSQHHEMFGFRRIERFVGTHSEGAELIDSLL
                     AELERFTGDNWEQEDDITLLTLQRLRH"
     gene            15621..17978
                     /locus_tag="VN178_14475"
     CDS             15621..17978
                     /locus_tag="VN178_14475"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWS82222.1"
                     /translation="MTALRSGLTRVWRYLRRGTAAYGVLLIALLLTVIAWYYVRQTVE
                     EQHRTRFEDSTQATQEALERRTKAYLDAMFGARGLYYASESVTRTDWSDYVKGIEPAG
                     RFQGLQALGYAQRVDPEQREAFLRMMRDEGLPGMRPDLTPGGERSVYFPLAHTGPLSE
                     ANQSMLNYDLYADKMHREAMDLARDSGEPRATKAVYVLSAAPESSIADLALRRGFFVY
                     LPIYQEEEPLGSVAERRRALRGFIVGSFKSDELLDGIFKGSFDPPIDFEVYDGGVKAP
                     NTLLYDRDGIRTDEKKRNEYLFFDESRIEVAGREWTLYFTTLQAFEEGAESNLPPFVL
                     VSGLLMSLLLFGITLLVVRSRTRAEHYSEDLEEANKELESFYYSVEQELGTARSIQHA
                     LLPKDLPKLEGWKIAYHYQPAREVGGDFYDFLNLDDGRLGIVIGDVSGKGIAAALVMA
                     NTQSVLRAVARRGDIAPGQVLAEANEVLYAYIPAGTFVTCFYGILDPKSGRLVYANAG
                     HDPPYLQRNGDAQELMARGMPLGLMPGMPYEEKESVLAAGDDLLFYSDGLVEAHDPEG
                     EMFGFPRLRKLIMSQSSGSGEELVDFLLAELTRFTGTDSEQEDDITLVTLERSRAGVR
                     DLEAPQPDGITGAAELRVLADFTLPSEPGNERTAMEKVSDAVQELPLSEQRLARLETA
                     VAESTMNAMEHGNRYDPEVPVRIQVWLLKERLLVRITDRGSGPLPSLRAKGPNLEAKL
                     EGSQTARGWGAFLIERMVDEVRVSGNPDHHTVELVMRLETGKDAG"
     gene            complement(18035..>18517)
                     /locus_tag="VN178_14480"
     CDS             complement(18035..>18517)
                     /locus_tag="VN178_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82223.1"
                     /translation="VLTKGHRICVQTFKEGHYFLPGVPDGPRIPEGYGKAGGLPNTHY
                     FGRAADVWWVDGKPVEGNATAPGILEVGKILAGIPPGRRPDQIIGPPGWTRDLGYGRQ
                     DGWVLAPDQLELHRDHIHIGYLDQSGPANSKRFRLLGVRRVYIARVDHLDNNRDEGSG
                     "
BASE COUNT         3614 a         5756 c         5821 g         3326 t
ORIGIN      
        1 tgcgtattcg ctcttcgaga tagcgtcgcc accgggcggc gggccaccgc cccacatcca
       61 gcaccgggaa gacgagtcct actacctgct cgagggggag tacaggtttt tggtagaagg
      121 ccgggccgtc aggataaacg cagggtcgct catctacgtc cccaggggca ccctgcacgc
      181 ccacaagaac gtgggagagg ggacgggcag gcttctgttg agccagacgc cagggggctc
      241 gcacgagcgc ttcttcgagg agataggcga gcccgcagtg gacggctcca ggccgccagt
      301 ccaggcagat cagcagcaca tggagaaaat catagccatc gcaaccgatt acggcatcga
      361 gataccgtcc cctgtccgtt cagacggcgg acagcaaagg atcccacaat gaatgcgtct
      421 gagctcctga ttacgagacg cagagatccg attgctgatg gccggaaagg agaaagtagc
      481 atgtttgcac ggatgttcac catcgaaggt cgccgtgagc aactggacga gttctcccgc
      541 ctcggagaga acaaggtcct gccggcgctg cagcggctcc acggattcga gggttcgctg
      601 gttctcgcaa accgccagaa cggccacatc ctgatagtgc ccttctggca aagcgaggaa
      661 acgatgcgcc gtgccgagga tgcctcgcac tggttccgcg gcttcgtcgc cgaagccgcc
      721 ggcggcgaag tgaccaacgt ggagaggtac gaggtcgtcc acccggaaac ggagggcaac
      781 gcagcatcgt ccagggcctg ggatcttcgc gttcctgccg gaaagtagcc gcttcgcgac
      841 acagatctcc tggaaaaccc gaagaatgcc catattcgcc cagaacccgg gtctactcga
      901 agcgttcacc gaatcgggtg gtatttcgac cggagagacg atcctcgtta ccggcgccgc
      961 cggcggcgat gatcgcccgc tggaaggacg cccgcggcat cgccctctga tccaggttgc
     1021 gcgatgccgc actcgacaat acactgtcct ggcacaaaac ctgagatagg tagaggctta
     1081 gaactccttc tcgaagatgg gtgcctgaga gggagtgagc cagtggcgca accggctacg
     1141 cgagatggaa ggttcggcgg tcggttgctt tgttgaagag gaaagagtca gaacgttcag
     1201 acaacaagcg gcttgctagg gctgtggctc agccgtccaa tcgcgccaat gttcgacgcg
     1261 aggccttctc cccaacgggc gtaaggctgc cgtggggggc cccggtcaat tacgcttctc
     1321 acacctttct cctggcgtag aggagtgctt cgatgcgtag actattcgcg ccccggcaac
     1381 tcggtgacgc gttaaagaag agcgaggccc ggtaccgcac cgttctggac gcggccttcg
     1441 acgcggtcgt gaccattacg accgacggtg tagtcaggtg gttcaatcgc ggggcagaag
     1501 caatcttcgg ctacagggca gatgaggtca tcgggcaacc ggtaatgctg cttatgcccg
     1561 agcgataccg ggatctctgt gaagccggtc tacagcacta cctgcgaacg ggcgaggcgc
     1621 gcgttgtggg cggcacgacc gagctcgtag ggctgcgtaa ggacggcagc gaattcccga
     1681 tcgagatgtc cctcggcgaa accaacgagc acggggagcg gctgttcacg gggatgttcc
     1741 gcgacgtcac cgagcgcaag cgaaccgagg atgcgctgcg cgaggcaaga gatcgcttta
     1801 gaggcatctt cgagaacgcg cccatcggcg tggccatggt caccctcgag ggccggtact
     1861 tgcaggtgaa tcactcgctc tgccgaatac tcgggtacac cgaggaagag ctgcaaaccc
     1921 tcacgtggca ggagatcacc caccctgacg atcttgcggc cagtcaggct tacgcgcgca
     1981 ggatagtggc aggcgagttc cccaggtacc acctggagaa gcgcttcctc cacgccgatg
     2041 gccacacggt gtgggcttcg ctgagcgttt ccctcgtcag ggactctgag ggcgagccgc
     2101 tctacttcgt ctcccagatc caggacgtca ccgagcgtaa ggaggtcgag agagcaatcg
     2161 tagagagaga ggagcgcttc cgctccctgg tacagtacag ctccgatatc atcacgatcc
     2221 tcgacgccga cggcacggtc cgctacgcaa gcccggcggt cgagaaggtg atgggctaca
     2281 aacccgaaga gcagataggg accaacgcat tcggcaccgt ccatcccgac gacagggatc
     2341 gagcgttgaa cacgttcgcc gaggtcctga aaagaccagg ccttcacccg cccctggagt
     2401 tccgggtgcc gcacaaggat ggctcctggt gctacctcga gcatgtcgta aacaacctgc
     2461 tcgaggaccc ggccgtgcgg ggtgtggtga tcaactccag ggacgtaacc gaacgcaacg
     2521 ccatgcagaa gcaactccat tatcaagcgt ttcacgatcc gctcaccggg ctgccgaacc
     2581 gggcgttgtt catggatcgc ctcgaacacg ccctgactcg tgcgaatcgg cggggcagca
     2641 agatcgcggt gctgttcatg gacctggaca acttcaaggt cataaacgac agcctcgggc
     2701 acaaagcagg agatcaacta ctcatagagg tcgccgatcg tctgaaggcc cccctacggc
     2761 ccgaggatac cgtcgcgcgc ctcggggggg acgagttcac tatcctggtc gaggacattt
     2821 ccggtttggg cgacgtggcc cggatcgcgg tgcggctcgc gcagaaactg cgacccccct
     2881 ttgcccttgg agaacaagag gtgtacgcca ccgtaagcac cgggatcgcc ctcaacagtc
     2941 ccgctcgaga gcagccggcg gatctcttgc ggcacgcgga tctggcgatg taccgggcca
     3001 aacacagggg caaggctcgc tatgaggtgt tcgagcccag catggacggc gaggccgtgg
     3061 agcgcctgat actggagacc gggctcaggc gagccctcgt acgagagcag ttcagggtct
     3121 actaccaacc catcgtggca ctcgagaacg ataggatcgc cggggcagag gcgctgttgc
     3181 gttgggagca cccgcagcgg ggtctgctac tgcccgagga gttcatttcg atcgccgagg
     3241 agacgggact gatcgtgcgg atcgggcagt gggttctacg agaggccggc aagcaggcac
     3301 gtatctggca agagcgctat cctggcacac tgcccttgac gatcagcgta aacctctcta
     3361 ccagggagtt tttccacccc aagctggtcg cggaggtcct aaacgagagc gaaatcgacc
     3421 ccgctagctt gcagctggag attacagagg gcgccctgag gaccaacggc acgtactcga
     3481 ccgaccgcac gctccgtaac ctcaaacgca tgggcgtcca gctcgcaatc gatgacttcg
     3541 gcttgggcta ctcctccctc tcttacctca agggcttccc ggtcgacttt ctgaagatcg
     3601 accgctcgtt catcgccggc ctcggagggg cgcccaacgg tgtctccacc aaagatacgg
     3661 agatcgtgaa ggcgatgatc gagctgaccc acgctttggg cctgaaggtg atcgccgagg
     3721 gggtggagaa tgccgaacag ctggcgcggt tgcggcagat gaagtgcgac ctcgcccagg
     3781 gaaactattt ctcagagccg cttccgagcg aagcgctggc ggtgaacctg gaacggaacc
     3841 ttacggaccg aggataggac gagaccgcgc cctatcttcc accccgccca ccgcaaacac
     3901 aggacagcat ccaccgcgtc gtcgccctgg gtcagtgcgc cacatcgggt atctatggca
     3961 ttatatctgt ggggacgcgg cagcaatccg gctagatcag ggagatgagt atgacgcgct
     4021 atgatccggc cgacggtcat agactgtgct gacgtgcgtt cccatcgggc tcggtggata
     4081 gcgcaccccg tacttgaaag gaggaacggt ggacaaccag ctgctagatc ttgtggtcct
     4141 ggccctctac ttcgccgtca tgatcggggc cggctactgg ggcctgagac gggctcgcag
     4201 cgccgacgac tacctggtcg ccggacggag gttgggccct ttcatgtaca tcggctgttt
     4261 gagtgcggtg gtgcttggag gcgcctcgac tatcgggggg gttgccctcg gttacgacaa
     4321 cggcatctcc gggatgtggc tggtcttctg gatcgggatg ggggtcatag ccctcggcgt
     4381 cctaatgtct agccgcctca gcagactcgg tgtgtacacc gtctctgaga tgctggagaa
     4441 ccgcttcggg gcggcctcgc gactgatcag cgcgatcatc atcgccgcct acgccctcat
     4501 gatcgccgtg acctccacca tcgccatagg caccgtcttc aacgtcgtac tccccctctc
     4561 ctccagcgtg gcgatactcg tggccggggg cctcgtcgtc gtctactcgg tggccggcgg
     4621 catgtggtcg ataaccctca cggacatcat ccagttctgc ataatgacgg tcggaatctt
     4681 cttcttgttg ctccccatct ctatctttgc ggccggcggc ctctccggga tgcgggaggc
     4741 actgcccgcc tcctacttcg acgtcacggc gatcggttgg cagacgatct tcacctactt
     4801 cctgctgttc ttcttcgggc tgatgatcgg acaggatatc tggcagcggg tgtttaccgc
     4861 ccgcagcccc gaggtcgccc gctggggaag cgttgcggcg ggcgtctact gcctgctcta
     4921 cgccttggca ggcgccctcg tggggaccgc ggcgcgggtg atcctccccg acctcgcgtc
     4981 gtcggacaac gccttcgcca ggatcactac agaggccctg ccagcaggga tcacgggcct
     5041 ggtcctggcc gctgcgctcg ccgccgtgat gtccaccgca agcgccggcc tcctcgcctc
     5101 atccaccatc ctcgccaacg acgtctacgc cggcttcatc gccaggggcg accacaacaa
     5161 agtactggtt agccgcgcga ctaccctact cgtcggcatc gtggtcctcg tcatctccct
     5221 gatcgtaagc gacgtagtag gcgctctgac ggtggcttac gacctactga gcggagccct
     5281 attcgtgccc attgtgggcg ctttgttctg gagacgcgct accgccgcag gtgctctgtc
     5341 gtccatggcc gcgggcagcg tcgtcgtggt gatcttcatg gtcttggacg gcctctttgc
     5401 gaacaccccc ataatctggg ggatgctggc gagcctcgtc gccttcgtgg tggtgagctt
     5461 gctgaccccc aggccctccg aggagcgcat gaggacgtgg gagcggcggt tgaccggctc
     5521 cggagccgaa ggagagatct aacggcccgt atacactcgg tctacagcgg gttgcaggag
     5581 gattgagctc ctgcaacccg cgccgtcagc cctcagctat cagctctttg ttcttgctga
     5641 aagctgaagg cttatagctc gtcgtgcaaa agggttcagc ccttttgcac gccgctgtta
     5701 tacgttccga gggcgtattc tacggccgca tcgaaggcca tcgccctacc cgcagcccgc
     5761 gcctcctcga aagcctcttc acctagacga gaacgtacgg cgaccatcgt gtgttcgagc
     5821 ggccggagtg aacgccttgg tctgtgagat ctataaagtg gtgcttcgac catttgcagc
     5881 agcccttcgg ccgcgccgaa cagccgcgcc gaacgctccg cttcgcctcc tgcctcggct
     5941 gccgcagcca gtccctccag tagataggcg agcccacccc gcagccccac ctgggcggcc
     6001 aggaagaccc cctcctcgaa catgcgcgtc gcggcagcgt agtcgccccg agccagtgct
     6061 atttgggcca ggaagtagag cgcggagtag gtgccgagtc tgtctcctct cttacgggac
     6121 tgtgccaacg ctctctccag catggtgagc gcccgatcta ggtcgtcctg acgtagcgcg
     6181 accattccga gcctgtcgta caccgcagat accatctcct cttcgccaga ctcgcggaag
     6241 agcggtagcg cttcttcaaa gcgagagatg gccttctcga agtcctcacg gtccaccgcc
     6301 tccaggccca accagaaccg aacgtatgct tcgcacaacg tgtccccagc ccgcttggat
     6361 agctctagcc ctgccgccaa caacccggtg gatctctcgc ggtcaccttg caagtaggcc
     6421 gtcatggccg cgatctgaaa agccctggga cgcacgaccg gtgggatctc tagctctagc
     6481 agccgctcta tccaccggtg gctctcctgc tggtggtcgc gcaaccacca gaacgtccac
     6541 aatgcccatc ccaggcgtgt ggcggtctcg acttcgtcct cggtgagggc ccacgacatg
     6601 gtcgcccgca ggttgccatt ctcctgctcc aacttctcca gccactcgac ctggcgcgaa
     6661 cccttcagtc ccggccgcgc ttcctcggcc aggtccagga agaaagcggc gtgtcgccgc
     6721 cgcatctctc cggcctcacc gctctcctcg agcttctcga gcgcatactg ccgtaccggt
     6781 tcgagcatcc cgtaacgttc ctcgctgccg ccggttttcg ccgttacgag cgactgctcc
     6841 accaaccgcc ccagcaaccc gagcacgtcc tctgtcgcca cgccggccgt cgtttctacg
     6901 gcttccgcgg cctccagcgt aaaacctcct gtgaaaaccg agagatgccg gaagagcgct
     6961 ctctccatct cggagagaag ctcgtaactc cagtctagcg ttgcgtgcat cgtcctctgg
     7021 cgctctggaa ggtcttgcgc ccatcccgca gagagtgcct gatccaggcg cgcaaggagc
     7081 gatgaaggac tcaggatcct cgttctggcc gccgccagct ctattgcgag cggcaaccca
     7141 ccgagccgcc agcagatcga cgcaaccgct gcggcctttt cttctccgag cgtgaaggca
     7201 gggacgatcg ccttggcgcg ctcgacgaac agacggccgg agggcgagcc gaggaccttc
     7261 ccggggtcag gcgaccgggt ggaggtgggc agcgcgagtg gttcgaccgg gtactcctgc
     7321 tcgccccgca ccctgagcgg cgcgcggctg gtggccatga tggcgagatg cgggcaggct
     7381 tccagcaagt aagcgacttc ggctgcggcc tccaacacat gctcgaagtt gtcgagcgtg
     7441 agcagcattc gtttgtctcg caggtaactc acaagcacgt cgcgagaggt tgcgcctcgc
     7501 ggctccgtca acccgagggt ccgaacgacg cccggcagga ccagggacgg atcgcccagc
     7561 ggggcgaggg ccgcgaatgc cacaccatgc ccgaagtgtt ccgccgagtc gtgcgccgcc
     7621 cgaacggcca ggctcgtctt gcccaccccg ccgacccccg tgagcgttag aatccgccct
     7681 tccgggcgat gcaggagatc caggacctcc cggagatccc gctcccttcc caaaagcggc
     7741 gtcggcgccg gtagtgtcgc gggcctgagg aaccctgcct ggccgtcttc gcgacccggc
     7801 accgccgcga cgagggcggc acgttccgcc tcggaaaggt ccagggcctc tgcgagcgag
     7861 cggaccgtgt gcgggtaggg gcggcgccgc tcgccgcgct ccagcgcgct cacggcgttg
     7921 cgggtcaggc cggctcgggc ggccagctcc tcctgcgtga gcccggcgga ctcccttagt
     7981 ctccgaagct tcgccccgaa cgatgacctc tgctcggcac ccatgaccag tctttgcccc
     8041 gtcaagatag ttcgccgtgc aagacccatg ttagcaaggc cgaaaagctc ctgcgacgaa
     8101 gagtgtgtgc gaggtgtgtg cgaggtgttg gtgggttccg ctcgctcgcg ggcgcagact
     8161 tgagaccagc aaccgaagat gcccgcggac gatcatacgc cgggcggaag taaggggaag
     8221 gagatcgtgg taccggaaga aatagcggga tcgggaagaa gaagcgccca tgcggctccg
     8281 gaacaggacc gcaggtcgct gtgggtctta ggcgagttgg tgacgtgcaa ggtcgcgagc
     8341 gcccagacga gcggggcgta ctcgctcttc gaggtgacat ctccgccagg gagtggtccg
     8401 ccggcccacg tccaacacag ggagaacgag gccttttacg tgctggaggg cgagtacgag
     8461 tttctggtcg aagacagaac cgtgcgagtc tccgccggat gtctcctcca cgtccccaaa
     8521 ggcaccctgc accgccacga taacgtgggg gacatgccgg cgaggatgct ggtgatccag
     8581 acgcccggcg gcctgcacca agccttcttc gaggagatag gcgaacaaac gtcagccaag
     8641 cgggagtcgc acgtcccgga gcgtccggag agccttgcga gaaccaggga gatcgcggcc
     8701 ggatacggca tcgagatact gcctgccgag aagcagaaca gggaaatgag tcgaagacag
     8761 aacgacggag ggaggagctt acatgaatag cgaacaggag agatgtccgg tacgcctgcg
     8821 catgctgcgg ggcgcggccg ttcgtgaccc gccggcccag gctgccacag gagaatcgat
     8881 gccgacccga tcgaaaggac cgaaacgaga tggctaagga aagaaccggc cagaggcaca
     8941 agaacaagat ctacttcgag gaccaggacc tggacctcta cctgcaggcc ttcccgctga
     9001 accacctggg ctacggtggc gccgctgcgg gcgaggtctt ctacgcggcc tctcgtataa
     9061 acgagaagga cctggagagc tgggttgcgg agtggagcat gctcgccacc cgcctcgagg
     9121 ctcaggccgc gaaggccctg cagagaaacc acatcgtgag cgcccgagag tcctacctgc
     9181 gcgccttcac ctatcgccgg accgccacgc tcgggttgcg cgctaccgac ccacgcttcc
     9241 gggcggaatg ggagacgatg cgttcatgct tccggcgggc tgcaaagctg ttcgagccgc
     9301 ccatcgaaac ggtcgagatc ccttacggtg atgccgtgct cccggcctac ttcgtgcggg
     9361 cgggcgacca gagaaggccg acggtcatcg tgatcggggg cggagagcag ttctgcgaag
     9421 agctctactt ctggggcggg gccgccgccg tggaacgcgg ttacaacgcg ctgctggttg
     9481 acatgcccgg ccagggcgga acgccattta cggggatgca ccaccgccac gacgttgagg
     9541 tgccgatgtc cgcagtcctc gactacctcc aagaccgtac tgatgtggac gcgggtcgcg
     9601 tcggagccat gggcttcagc ctgggcggct acattgtgct tcgctcggtc gcccacgaca
     9661 agaggatagg cgcatgcgcg gtgagcacgc ccatcatcga ctggcaccag accctgatgg
     9721 atgcgttgcc caccgtcctc aggcgggccc cgaggcctct gttcaacgcc gtcatgaagc
     9781 tcgggaactt cttcggcaag acccagctcc tcgcctacga gaagttcttc gagtggcagg
     9841 ttggggcagc gagctacacc gaagcgttcg acagcttccg tccctggaag gtggacatcc
     9901 agagcatctc gtgccccgtg ctgtgcatga tgggcacggg cgaagcggcc acgttcaaga
     9961 aacagaccca agcctgctac gcggcgctca ggagccccaa aaccctacgg gtcttcaccg
    10021 aggaagaggg cgctgacgcc cactctcagg ccaacaatca gagactcgcc caccagacca
    10081 tcttcgactt cttcgacgag accttcgcgc gggacgaggc aaatcgcgag cttgggctgg
    10141 cgcaaggagc ataacgctca gtgcgcccag ccgtcgttgt cttgggcgaa aagcgttagg
    10201 gaaggagggc atacgatggc aagaacgacg atgttccagc gcagtcgcac gaaggtgcgc
    10261 ttctcgaatg aggacatgga ttacctcttc caggtagtgc gcggctacca cacccacgga
    10321 ggggcgagct tcggcgagtt gttccatgcc gccgccaagg tggaggaggg cgatccggag
    10381 agttggatct ccgccttccg ggagatgggg gagcggatcg agcgccaggc cgaggaactc
    10441 gaagggcggg gcaaagcgag cgccgctcta gcgttcttgc gcgccttcac cggctaccgg
    10501 gccgccgcct tcctgatgaa tcccaaggcc gacatcgagc gcttcaccga gaccgtcgag
    10561 tccttcgtgc ggtgcttccg gaaggctatc ccgggcttcg gtaacccttg tgagccgatc
    10621 cgtatcccct tcgggggtag ctatttgtcc ggctacttct accaggtgaa cgagcaaacc
    10681 gtgccccgcc cgaccgtgat cgtagtcggg ggcggcgata cctacgccga agacctgcac
    10741 ttctgggctg gcgcagcggg ccccacgcac ggctacaatg tgctctcgat cgagctcccc
    10801 gggtacggcc tgagccccac gcgtggtctc cacctcttcg ctggcctcga agcccctatg
    10861 gccgccgccg tggactacgc cctcagcagg ccggaagtgg atgcagaacg cctcgccgtc
    10921 tacggcatta gcggcggcgg gtacgcggtg acccgggccg ccgctaccga ggaacgtatc
    10981 cgcgcgacgg tcgccacgac gcccatctac gacatctacc ggctcatcac cgaggccatc
    11041 ccgaactttt tgctcgacga gtcgcacagc ggcgtctcgc gctggctgtt agagatatcg
    11101 ggtaggttca acaaggccgg caagaacaac atggacaagt tcgcctggca gtcgggcaag
    11161 ggatcgctcc tggaagccgt ccgtgttacc gagggcgaac ccgtggacgt tggggcgata
    11221 cagtgtccca tgctgtgtct ggcagcagag ggtgacccgc cggaggcgct cgggcagacc
    11281 aagttcgtct tcgaccacct ggagcacccc aagaaggcga tgcgcgttct gacggccgag
    11341 gagggtgccg aggcgcatac ccacgtcaac aacttcccgc tgttgcacca ggtcgtcttc
    11401 gactggctcg acgagacgct cacgccaaag gacataaccc aaagagtcag gctggcggat
    11461 ggagcatgac gctcgttcct cgcgttggca caagctggag gatcactgcg tggctcgacg
    11521 ggacttctca gcgaccatcc ggcggtgtac caggcccgaa cgacgtgagt gacgtgttag
    11581 ggtgagcgca tggtcaccac aggttaagcg acgagaacga taccgaggag agcatgagag
    11641 caaccgtata catcgcaaca agcgtcgatg gctttatcgc gcgggaggat ggcggcatcg
    11701 actggcttcc cgacgagggc gatgaggagg gtggcgagga ttacgggtac caggagttta
    11761 tagactctgt ggatgcgatc gtcatgggac ggagcaccta cgagttggcg ttgtcctttg
    11821 gttcgtggcc ttacggcgaa aagcccgtga ccgttctcag cagtcggcac gtagaaatcc
    11881 ccgacgccat cgcccgtacg gtcgaggcca tgtcggtggc gccgcgggaa gtggtccagc
    11941 gtcttgcaga gcgcggatac gggcacctgt acgtcgacgg cgggaggacc atccaaggat
    12001 tccttcgcga tgggctgatt caccaactga tcctcacgaa gatccccatc cttatcggta
    12061 ccggcatccc gctgttcgga ccgctttctc acgacatcgg attgcgccat ctcgaaacgc
    12121 ggcagttcga gaacggcctg gtgcaaagca gatacgaggt ccttgggaac gtcacttgat
    12181 tgcggctgtg atccgccggc gaacattctt gcaaatatga ggaccacgtt gtgggcgttg
    12241 attgtctacg cttcacgagt tacggtaggg aggtgttgtg ccacgatgga aaagcttcag
    12301 aacgcgttgc gggtgatctt cgcgtcgacc atagtgcaat gctgtgaagt gatcgatggt
    12361 aaccttgccc gctggcgcgg cggtcgcgtt gcggctcgtc gcgggagaac ctgacacctg
    12421 agcgcagggg tttttcaaga agcctatcgt ctggtcgaag tcgaaccgcc tgcccatgcc
    12481 gtgataacgt tcagtgcaag aatcactcat ccggatgatg cgtcggcctc gtacgtaggt
    12541 taagctcttt tcgttgtccg gaaggatgtg taatgagcac accgccggat agggactccg
    12601 agtcagcgga gagtaaggaa agaccaacga tcgtaaccgc accacgacgg tagagcacac
    12661 agaggatgaa agcgagcaca gcgcggaaaa cacgcccacc acagaatggt gccaacacgc
    12721 gtctcgttcg gcgtgccgcc ggcttgatcc tgctgttcaa cggcctgctc gttgccctgg
    12781 tgctcttcaa acctataagc gacgtcacac tcgccctcgt ggtcaacgcg gcgcagttca
    12841 ttggtccgct gctcgcgcta cccttgtgct tcggtggact actaaggcgc gtgtgggagc
    12901 gaggagcttc ccaagcgcac gtggggactg cggtgaggag agggcagcgt tgggctcccg
    12961 tcttgcttgg cctgggcatc ctcagttggg tattcggaca gatggtcttc acttattacg
    13021 agtgggtgct gggccggccg ccaccgctgc cctctatcgc cgacataggc tatttgagcg
    13081 tatacccttt cttgcttttg ggcatactgt ttctaccttc ccgcccgatt ccggtcgcat
    13141 ctcgcacccg catagcgctc gatggcctga tgtccatgac ggcagccgtc accttcagct
    13201 ggtacttcat cctcgggccg atcatgcagc aggggtccga gacgatactc gcgaaggccg
    13261 tgtcctcggc ctacccactg gccggcatct tcctgatcgc ctgcctgata atactcgctt
    13321 cgcgacctga ggagctcact ctcctacccg ccgtgcgcat cttggcgctc ggcctgaccc
    13381 ttatcgtgat agcagacagc aacttcgcgt actggtcgct gcacgacgcc tatgccacgg
    13441 gaacgctccc cgatataggc tggtcgctgg ggtacatgct cgtagccctg ggcgccttcg
    13501 ccgcacggct ggcccctggc gaggaggtga cgatgacacc aggggagtat gtcgatacgt
    13561 ctggcaccgc atctcctctc cccgaacagc gagtgtggac cgcgctgatg ccttacgttc
    13621 tcgtgcccgc ggtgggcatc ctcgtcgtct atgcctggcg acaatctgcc gggagcggca
    13681 gcctggcgac aggagtttat ctcgggggag cgctgctcat cggtctgatg gtgttgcggc
    13741 aggtcctcac catcgtggag aacgcgcggc tctacaaccg gctgcaacgc acccacctac
    13801 aggtggaaca gaaaaacgac cagcttgtgc gctcccagag tgaattgcgc cggcagaagg
    13861 aatacttcga gtcgctggtg ctaaacagcc cagtggcgat cgcgatcgtg gacctcggtg
    13921 gaaatgttgt ttcgtggaac ccggcggccg agagattgtt cggtttcacc caagcccagg
    13981 ccatcgggcg cagtattgac gacctgcttg ccgggacacc acagatgcac gcggaagttt
    14041 tggagtacac acggcagacc gcgagcgcca ggcgagtcga cgtcgtcact cggcgtagcc
    14101 gcaaagacgg aacactcgtg gatgtggaac tcctggccgt tccggtgaac gtgggcgggg
    14161 accatgtggg cacttacgcg atgtatcatg acatcaccga gctcaagcgg gtagaggaag
    14221 aggtcaggca gctaaatagg gacctcgaga agcgggttgc agagcgtacc gagcaactca
    14281 agagcgccat ggccagagaa caggaggtgg ccaaggagag ggagcgcatc gaacaggagc
    14341 tacgggtcgc gcgaatgatc cagcacaccc tcctgccgaa gtcgctgcct gagctcgaag
    14401 ggtataagat cgcggtccac tatcagcccg cacgtgaggt tggtggcgac ttctatgacc
    14461 tccttcagct gccggatggc cgcctgggct tgatcatagg ggacgttagc ggcaagggcg
    14521 tgcctgctgc catcgtgatg gcgatcaccc gcacgatgct gcggtcggcc taccatctgg
    14581 gctcgcccgg agagatacta caaagggtca acgacgaact ctttccggat atccctccca
    14641 acgtgttcgt cacctgtctg gcagccctac tggatagcaa gaccggtcgg ctgcagtacg
    14701 ccaatgccgg acacgaccca cccttcgtgc gccacgccag cggtgtcggt aaactccggg
    14761 ctaccggcat gcccctgggc ttgatggctg gcatggttta cgaacagaag gagatcaccc
    14821 tcgagcccgg ggagggcgtc ctcctctaca gcgatgggct ggccgaggcc cacagccaac
    14881 accacgagat gttcggtttc cgacgaatag agaggttcgt cggcacacat tcagaaggtg
    14941 ccgagttgat agattctctg cttgccgaac tggagcggtt caccggagat aactgggaac
    15001 aagaggacga cattaccttg ctgacgctcc agagacttcg acattagtgt caggcgaaag
    15061 agttacagcc cattttcgga gccgaccaca gacgccggcg aggcgaagtc cagcgcctac
    15121 aagtggcctg ccctagagcg gcgaacctct ttgacttcga gctcctggat cagctcgcgg
    15181 cggtcgagac gtgcgaatga gccgggtgca agtctccttg tggttgcacc ccgacgagct
    15241 tcgaaacggc gagagggcag aatagcgaat ccttattgac cgccagcctc gacaggcgcg
    15301 accggtaatg tgatggaaag tgtaggccgg gcgaggttca acgcaccctc ggctacccga
    15361 cccaccgggc accaaaccct ccaaccgatt ccgtataaac caggcttcgt aacggttttc
    15421 gtaacgaacc agagacgcta acggtgtagg atggggtgag taagtgccta gagatgggac
    15481 gaacaggtgc gtagaggtga taccgacagc ccacgactga aggacgaatc tgcggtgaac
    15541 ctggcaagaa ctcctgtaac tccaccagaa atcacgagac ctcgcggcca ggggttgagg
    15601 tgagtcggtc cgatggcggc ttgacggcgc tgcggtccgg actcacccgt gtctggcgtt
    15661 acctgcgccg cggaacggca gcctacgggg tgctgctcat agcactcctt ttgacggtca
    15721 tcgcctggta ctacgtgcgc cagaccgtcg aggagcagca ccgcacccgc ttcgaagata
    15781 gcacccaggc cacccaggag gctctggagc gccgcaccaa ggcgtacctc gacgctatgt
    15841 tcggcgcccg cggcttgtac tacgcctccg aatcggtcac cagaacggat tggagcgatt
    15901 acgtcaaggg catagaaccc gccggtcgat tccagggtct acaagccctg ggctacgccc
    15961 agcgggtgga ccccgagcaa agagaagcct ttttgcgcat gatgcgggat gaaggtctgc
    16021 ccggtatgcg gcccgacctg acccccggcg gggagcgttc cgtgtacttc ccccttgccc
    16081 acaccggtcc cctcagtgag gccaaccaga gcatgctcaa ctacgatctc tatgccgata
    16141 agatgcacag ggaggccatg gatttggcgc gcgattccgg agagccccgg gccacaaagg
    16201 cggtctacgt gctcagcgca gcccccgaaa gctccattgc agacctcgcc ctgcgtcggg
    16261 ggtttttcgt ctacctccct atctaccagg aggaagagcc gctggggagc gtcgctgagc
    16321 gccggcgggc cctcagaggg ttcatcgttg gatctttcaa gagcgacgag ctgttggatg
    16381 ggatcttcaa gggatcattc gacccgccga ttgatttcga ggtctacgac ggcggggtta
    16441 aggcgccgaa cacgctgcta tacgaccgcg acggcataag gaccgacgag aagaagagga
    16501 acgagtatct cttcttcgat gagagccgga tagaggtagc aggccgcgag tggaccctgt
    16561 actttactac gctgcaggca ttcgaagagg gggccgagag taacctgcct cccttcgtac
    16621 tcgtcagcgg cctgctgatg agcctgctgt tattcgggat caccttgctg gtggtgcgta
    16681 gccgcaccag ggccgagcac tacagcgagg acctcgagga ggcaaacaag gaactggaga
    16741 gcttctacta ttcggtcgag caggaactgg ggacggcgcg aagcatccag cacgccctgc
    16801 tccccaagga cttgcccaaa ctcgagggtt ggaagatagc ctaccactac caacccgcaa
    16861 gagaggtggg gggcgacttc tacgatttcc tcaacctgga cgacggtcgt ttgggcatcg
    16921 tcatcggcga cgtctcgggc aagggtatcg cggcggccct ggtgatggcc aacacccaga
    16981 gcgtgcttcg cgccgtcgcc cgaaggggag acatcgcccc aggccaggtg ttggcagagg
    17041 ccaacgaggt cctgtatgca tacatcccgg caggcacgtt cgtcacctgt ttctatggca
    17101 tcctcgaccc aaagagtggc cggctcgtct acgccaacgc cggccacgac cctccctacc
    17161 tgcagcggaa cggagatgca caggagctca tggcgagggg catgccgctc ggcctaatgc
    17221 cgggcatgcc ttacgaggag aaagagtccg ttctcgcggc gggggacgac ctcctcttct
    17281 acagtgatgg gctggtggag gctcacgacc ccgaaggcga gatgttcggc ttcccgcgcc
    17341 tcaggaagct gatcatgtcc cagagctccg gtagcggcga agagctcgtc gactttctgc
    17401 tggccgaact gacccgcttc acgggaacgg actcggagca ggaggacgac atcaccctgg
    17461 tcaccctgga gagatccagg gccggagtga gagatctcga ggccccgcaa ccggatggga
    17521 taaccggtgc tgcagagcta cgagtcctcg ccgactttac ccttcccagc gagccgggga
    17581 acgaacgcac ggccatggag aaggtctccg atgcggtaca ggaactgccc ctgtccgaac
    17641 agcgtctggc aaggctcgag accgccgtgg ccgagtcgac gatgaacgcc atggagcacg
    17701 gcaacaggta cgatcccgaa gtccccgtca ggatccaggt atggctcctg aaagaacgcc
    17761 tgttggtgcg catcaccgac cggggtagcg gccccctacc gagcctgagg gcgaagggac
    17821 cgaacctgga ggccaagctg gaggggtcgc agacggcgcg gggctgggga gcgttcctga
    17881 tcgagcgcat ggtcgacgag gtacgcgtct cgggaaaccc agaccaccac accgtcgagc
    17941 tggtgatgcg cctggaaaca ggcaaagatg ccggctaagc gtccggggcc cgtcgtttgc
    18001 acggtgtggt cgtcccggat cgtgctagcc cacactatcc agatccctcg tccctgttgt
    18061 tatcgaggtg atctacgcgt gcgatgtaga cccgcctaac ccccaagagc cgaaacctct
    18121 tgctgttagc cggaccactc tgatcaagat aaccgatgtg gatatgatct ctatgcaact
    18181 ccagctggtc gggggccagt acccagccgt cctgacgtcc gtaccccaga tctctagtcc
    18241 acccgggcgg tcctattatc tggtcaggcc tcctcccagg cggtatgccg gcgagaatct
    18301 tgcctacctc caggatgccc ggtgcggtgg cgttgccttc gacgggtttg ccgtcaaccc
    18361 accacacatc ggcggccctg ccaaagtagt gggtattggg cagaccgccg gctttgccgt
    18421 agccctcggg tatgcgtggg ccgtcaggga cgccggggag gaaatagtga ccctccttga
    18481 aggtctggac acagatcctg tgccctttgg tcaggac
//
LOCUS       DATMVF010000278         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_636531, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000278 DATMVF010000000
VERSION     DATMVF010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636531"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="VN178_14485"
     CDS             complement(<1..99)
                     /locus_tag="VN178_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWS82224.1"
                     /translation="MEGKTRRPVTVVTGFLGSGKTTLLARVLSAPSM"
     gene            complement(104..475)
                     /locus_tag="VN178_14490"
     CDS             complement(104..475)
                     /locus_tag="VN178_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82225.1"
                     /translation="MCVLCGQDFVARVHWTDRHVEDRARAAEPGFDPVAFQGDRRRDR
                     AHRVVLTNEVLRHYGLRVDDWSGSKYVLRDRKGRSEIIQDLGSLWPAAAKLADRTLDP
                     LDPVLWERLGGEPAFAAADSG"
     gene            complement(494..1444)
                     /locus_tag="VN178_14495"
     CDS             complement(494..1444)
                     /locus_tag="VN178_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HWS82226.1"
                     /translation="MAEKEIFCSIGIDIDAVAGWLGSYGGEDSPDDISRGMFSGEVGT
                     PRLLKLFEKYDLRTTWFIPGHSIETFPDQVKMVVDAGHEVGSHGYSHENPVAMTPQQE
                     RDVLEKCLELIENVAGRKPVGNVAPWWEVSATTNELLLEYDYKYDHSLQHRDFTPYYA
                     RVGDSWTKIDYSKTAEEWMKPLVRGEEIDLVKIGGNWYLDDLPPMMFIKGSPNSHGFV
                     NPRDIEELWRDEFDWVYREMDYATYCLTIHPDVCGRPQCLLMLERLIEYINGHDGIRW
                     VTMEEIADDFVQRYPRSQPNHDPGINMYGVETPAAEGVSS"
     gene            1630..>2407
                     /locus_tag="VN178_14500"
     CDS             1630..>2407
                     /locus_tag="VN178_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HWS82227.1"
                     /translation="MALPRVAVFSMGGTISSTDSGGKGVEPTLTGEALVSDVPKIAEV
                     AEVSAISFRQAASGELTVDDLVELASEIEDRIDEGASGAVVTQGTDTIEETAFVLDLL
                     VDRNAPVVVTGAMRNPTLPGADGPANLLASIQVAVSEAARELGAVVVLNDEIHAARFV
                     RKTHTSNPATFRSEPVGPVGWVSEDIPRVVLRPVGRHKITLPGDAQDRPVALHTFTLG
                     DDGRLLPEIERQGYAGLVVEALGGGHVPSITVDALEDLAGK"
BASE COUNT          406 a          771 c          776 g          454 t
ORIGIN      
        1 catcgacggc gccgagagca cccgcgcgag aagggtcgtc ttgccgctgc cgaggaaacc
       61 ggtaacgacc gtcaccggac gcctggtctt gccctccatg agcctacccg ctgtccgccg
      121 cggcgaacgc cggctctccg cccagcctct cccacaggac cgggtccagg ggatctaggg
      181 tccggtctgc gagcttcgcc gcggcgggcc agagggagcc gaggtcctgg ataatctccg
      241 aacgcccttt gcggtcgcgg agcacgtact tgctcccgct ccagtcatca acgcggaggc
      301 cgtagtggcg cagcacctcg ttggtgagga cgacccgatg ggcgcggtca cggcgccggt
      361 cgccctggaa cgcgaccggg tcgaagccgg gctcggcagc ccgcgcccgg tcctcgacgt
      421 gccggtcggt ccagtggacc cgagcgacga aatcctgtcc gcaaagcacg cacatagaag
      481 aagtctcctt ctcctaggag gagacccctt ccgcggccgg ggtctcgacg ccgtacatgt
      541 tgatgccagg gtcgtggttc ggctgggacc tcgggtagcg ctggacgaag tcgtcggcga
      601 tctcctccat cgtcacccag cggatgccgt cgtggccgtt gatgtactcg atcaggcgct
      661 cgagcatcaa gaggcactgc ggcctgccgc acacgtccgg gtggatggtc aggcagtacg
      721 tcgcgtagtc catctcacgg tagacccagt cgaactcgtc gcgccacagc tcctcgatgt
      781 cgcgcgggtt gacgaagccg tggctgttcg gggagccctt gatgaacatc atcggcggca
      841 ggtcgtcgag gtaccagttg cccccgatct tgacgaggtc tatctcctcg ccacgcacga
      901 gcggcttcat ccactcctcg gccgtcttgg agtagtcgat cttggtccac gagtcgccca
      961 cccgcgcgta gtacggggtg aagtcgcggt gctggagact gtggtcgtac ttgtagtcgt
     1021 actcgaggag aagctcgttg gtcgtggcgg agacctccca ccacggcgcc acgttgccga
     1081 ccggcttcct gcctgccacg ttctcgatca gctccaggca cttctccagt acgtccctct
     1141 cctgctgcgg tgtcatcgcc accgggttct cgtgggagta gccgtggctc ccgacctcgt
     1201 gcccagcgtc aacgaccatc tttacctgat ccgggaacgt ctcgatggag tgccccggga
     1261 tgaaccaggt cgtcctgagg tcgtacttct cgaagagctt gagcaaccgc ggcgtaccta
     1321 cctcgccaga gaacatgcct ctcgagatgt cgtcgggcga gtcttcacca ccgtaggacc
     1381 cgagccagcc tgcaacggcg tcgatgtcga tgccgatgga gcaaaagatt tccttctcgg
     1441 ccatacaccc cacctttccc tttcgtcttc cccttttgtc tgaccaaagt tattgtacaa
     1501 gagaccgacg ttcacgctca acctgagata cggtcgctcg cgtacccgcc cggcgtttga
     1561 tcggcgcttc cagccccgtt aatatatttg tcagactaat ggggtaaagt ggagacgaga
     1621 ggaggagctt tggcacttcc gcgggtggcg gttttctcca tgggaggaac catctcttcg
     1681 accgattccg gggggaaagg tgtagagccg acgctgaccg gggaggcgtt ggtctcggac
     1741 gtgcccaaga tagcggaggt tgccgaggtc tcggcgatct cgttccgtca ggctgcgtcc
     1801 ggtgagttga cggtggatga tctcgtcgag ctcgcctcgg agatcgagga ccgcatcgac
     1861 gagggggcga gcggagccgt cgtcacgcag ggcaccgata ccatagagga gacagctttc
     1921 gtgttggacc ttctggtgga tcgcaacgct cccgtggtgg tcacgggggc gatgcgcaac
     1981 cccacgctcc ccggcgccga cggcccggcc aacctgctgg cttcaattca ggtcgcggtg
     2041 agcgaggcgg ctcgggagct tggcgcagta gtcgtcctga acgacgagat ccacgcggcg
     2101 cggttcgtgc gcaagacgca cacctcgaac ccggcgacct tccgctccga accggtcggg
     2161 ccggtaggct gggtttccga ggacattccc agggtggttt tacgcccggt cggacggcac
     2221 aagataacgc tgcccggaga cgcacaggac cgtcccgtgg cgctgcacac cttcactctg
     2281 ggcgacgatg ggaggctgct tcccgagata gaacggcagg gatacgccgg gctcgtggta
     2341 gaggcccttg gcggcgggca cgttccctct atcacggtgg atgctctcga agatctcgcc
     2401 gggaaga
//
LOCUS       DATMVF010000279         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_80960, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000279 DATMVF010000000
VERSION     DATMVF010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80960"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VN178_14505"
     CDS             <1..843
                     /locus_tag="VN178_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWS82228.1"
                     /translation="CGYDGIDVIFGKIQEIPEGEYPAFAEEFPAKLRELGLEFGYIGA
                     HSTFVSPRHFDRERGIAGFKKAVDAAADLGASSVCTLLGDGYYDPPLNILLSRKDAWA
                     QAVGAVGEVATYAADKGVNVSIELLQGSIVNRVPLLLRLIDEVDRENVRATVDTGTFY
                     TTVKPFVGVKEAIMELGDLIDIVHVKDEIGLPSIAQTNHTWMGGGLVDFAETREALEE
                     IGFSGYCSVEWEGWQVGGNIGVGEPAGLGLADFDRVVVEAKEYLEEFGYKSMRDKTAR
                     TGKE"
     gene            848..2035
                     /locus_tag="VN178_14510"
     CDS             848..2035
                     /locus_tag="VN178_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWS82229.1"
                     /translation="MTRENLASARMLGRMSRRDFLRIGGTSLAGASLLGLAGCGAGGG
                     TSSGKVPLILWHQEQPPNRVKQFQKVIDAFNKSQDNFKVTQQVQNYEDVYQKITSAIQ
                     AGEPPDIHFSIPDLTVAIMETGAVQPVEDIVKEIDSSHPFLPAALQPYENEGHTWAVP
                     IFGMIQMLWYRKDMFEAAGLDPESPPRNWDELRQYSEQLTQGNEHGIGITSSKHLYTD
                     QEFYTFMITNGGKEMFDESGNVNFDTPNNVETLAFYKELSQFSPPGSNSWTWAEPQAA
                     LNNGSLAMAIEKGQFLGPFEEESGRPASDLGVAPVPWPPDGERGSIYYSNAAMTYTQE
                     EEKLEGVKEFMTFILEPKNYADILLAEPGLFLPVTEDGDSPAWKDSEVLGKYPEAVNL
                     MVE"
BASE COUNT          454 a          651 c          712 g          357 t
ORIGIN      
        1 tgcggatacg acggcataga cgtgatcttc ggaaaaatcc aggagatccc cgaaggcgag
       61 tacccggcct tcgccgagga gttccctgcc aaactacgcg agttgggact cgagttcggt
      121 tacatcgggg cgcactcgac cttcgtctcg ccgcgccact tcgaccgtga gcgtgggata
      181 gcggggttca agaaggccgt tgacgccgcc gcggacctcg gggcgagctc ggtctgcacc
      241 cttctcggcg acggctacta cgacccgccg ctgaacatcc tcctgagccg caaggatgcc
      301 tgggcgcagg ccgtgggggc ggtgggcgag gtcgcgacct acgcagccga caagggtgtg
      361 aacgtgagca tcgagttgtt gcagggaagc atcgtgaacc gggttccgct cctgctgcgg
      421 ctcatcgatg aggtggaccg ggagaacgtc cgggcaacgg tggacaccgg gaccttctac
      481 accaccgtaa agcccttcgt gggggtcaaa gaggccatca tggagctcgg cgacctcata
      541 gacatcgtgc acgtgaagga cgagatcggg ctgccctcca tcgcgcagac caaccacacc
      601 tggatgggcg gcgggctggt ggacttcgct gagacccgtg aggccctgga ggagatcggc
      661 ttctccggtt actgtagcgt cgagtgggag gggtggcagg tcggggggaa cattggcgtg
      721 ggtgagcccg ccggtctggg gctggcggac ttcgaccggg tcgtggtcga ggcgaaagag
      781 tacttggagg agttcggcta caaatcgatg cgtgacaaga cagcgcgtac gggaaaggag
      841 tgagaagatg actagagaaa atctagcgag cgcacggatg ctcggcagga tgagccgccg
      901 ggacttcttg aggatcgggg ggacgagcct cgccggggcg tccctcctag ggctcgccgg
      961 ttgcggtgcg ggtgggggga cctcgagcgg taaggtgccg ctgatccttt ggcaccagga
     1021 gcaaccgcca aaccgcgtca agcagtttca gaaggtcata gacgccttca acaagtccca
     1081 ggacaacttc aaggtcacgc agcaggtcca gaactacgag gacgtctacc agaagataac
     1141 ctcggctata caggcgggcg aaccacccga catccacttc tccataccgg acctcaccgt
     1201 ggcgatcatg gagaccggag cggtacagcc ggtggaggat atcgttaagg agatcgactc
     1261 ctcacatccg ttcttgcccg ccgccttgca gccctacgag aacgaggggc acacctgggc
     1321 ggtgccgatc ttcggcatga tccagatgct ctggtatcgc aaggacatgt tcgaggccgc
     1381 cggcctcgat ccggagagtc cgccgcgcaa ctgggacgaa ctgcgccagt actccgagca
     1441 gctcacccag ggcaacgagc acggcatagg catcacctcg agcaagcacc tctacaccga
     1501 ccaagagttc tacacgttca tgatcaccaa cggcggcaag gaaatgttcg acgagagcgg
     1561 gaacgtcaac ttcgacacac ccaacaacgt tgagacgctg gcgttctaca aggagctctc
     1621 gcagttctcc ccgccgggga gcaactcctg gacgtgggcc gagcctcagg ctgctctcaa
     1681 caacggctca ctcgccatgg ccatcgagaa gggccagttc ctgggaccct ttgaggaaga
     1741 atcgggccga cccgccagcg accttggcgt cgcgccggtg ccgtggccgc cggacggcga
     1801 gcgggggagc atctactact cgaacgccgc catgacctat acccaggagg aggaaaagct
     1861 cgagggcgtc aaagagttca tgacgttcat cctcgagccc aagaactacg cggacatcct
     1921 tctcgccgaa ccgggcctgt tcctgcccgt gacggaggac ggcgactccc cggcctggaa
     1981 agactcggag gtgctcggga agtatcccga agcggtgaac ctcatggtcg agtagtccaa
     2041 gtacgggtac ctcttcggct tcaccggcga ccagatcccc gaagggatcg gccagatctc
     2101 cgctgagaac atgctctctc aggtcgtgca gaaggccatc gtcgagggcg attcgcctga
     2161 gagtgcggtc gcct
//
LOCUS       DATMVF010000280         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_225429, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000280 DATMVF010000000
VERSION     DATMVF010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225429"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VN178_14515"
     CDS             <1..465
                     /locus_tag="VN178_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWS82230.1"
                     /translation="VKVRIPAGAKDGMKVRIPGRGSAGRRGGPAGDLYVVTRVEEHPV
                     FRRRGDDFVVEVPVSFVEAALGAQIEVPRPGGGTVKVKLPAGTQDGKQFKVRGAGAPR
                     VRNDGAQKGNLIVRVGVVVPKKLKRREREILEALAEERDEDVREDLFKKVGS"
     gene            462..863
                     /locus_tag="VN178_14520"
     CDS             462..863
                     /locus_tag="VN178_14520"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWS82231.1"
                     /translation="MSLRKRPVYMIGIASELIGVHPQTLRMYEQKGLLRPRKSIKNTR
                     LYSQEDVDLGRYIQRLTQEMGMNLAGVGKVIDLERQVAKLKAEVNALREELDRKEREH
                     SRLISEVHQSYKRELVLMPRGELARSGRKDR"
     gene            863..1348
                     /locus_tag="VN178_14525"
     CDS             863..1348
                     /locus_tag="VN178_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82232.1"
                     /translation="MAEPMHCYRHPKRETRVSCATCGRPICTECMVATDVGIKCPDDA
                     RLPRGARAGVMKTDQVVKSILAGAGVALAGIPVAYVLFLLPFTWLISAAAGYGAGVLI
                     NRAGGRNGGPLAIVISVLATAVPFLVLLAPDLLAGALNPRPLIAMVFAVIAAGIANRR
                     I"
     gene            1783..2322
                     /locus_tag="VN178_14530"
     CDS             1783..2322
                     /locus_tag="VN178_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82233.1"
                     /translation="MREDMQYISGVLDELEAIFQDASGVPMSKGRAVVDRSDLLVMLD
                     ELRGSLPPELAEAEALRRECGATVAAAEEEAQRIIEEAHQRADALVPETELLRRAERR
                     AGEVIDGAERYAEEVSGGSEVYRDRVMGQLENWFQDSLISVEESRQELGGVPVRRPAP
                     RSEVVDEETDGQGWRASSA"
     gene            complement(2556..3338)
                     /locus_tag="VN178_14535"
     CDS             complement(2556..3338)
                     /locus_tag="VN178_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82234.1"
                     /translation="MPERRNSGAGPPVDRENATLAPGERNRMLRAAALRPINVTMLVI
                     GAGAFLTTLAWWLVPLTILTYAALVFLAARDPIFQRKVLGRGEVPKTSPNAVESTELS
                     PERRARWLPRGETRQRVEAALGVYRKVVAAIETSDDVTRSVLDDAVPRLHAAADRLVD
                     VAQGRERAAEVARDLGQDTASATPTTARQENLRELENRISAADAEISDTYQELLDLRA
                     KVVRISIDSENPARTKDLNASLDGLNARLEALSELMVPQEES"
     gene            complement(3331..>3681)
                     /locus_tag="VN178_14540"
     CDS             complement(3331..>3681)
                     /locus_tag="VN178_14540"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HWS82235.1"
                     /translation="PSSLVGLGPANVRIDEGAGLVTHIISVDRFSNARLSIMQEESGL
                     EYGDALKVDAGDGGMAVRYVETFGSAKAGELVLVPDSHWRLSLAINKGSAAQALALEI
                     GGKVRLILPADHGA"
BASE COUNT          685 a         1064 c         1243 g          689 t
ORIGIN      
        1 gtcaaggtca ggattccggc cggggcgaag gatggaatga aggtaaggat ccctggcagg
       61 ggaagcgcag gcaggagagg tgggcctgcg ggagaccttt acgtcgtgac gcgggtcgag
      121 gagcatcccg tgttcaggcg gcgcggcgac gactttgtgg tcgaggttcc tgtcagcttt
      181 gtggaggcgg cgctgggggc gcagatcgag gtgcccaggc ccggcggcgg cacggtgaag
      241 gtgaagttgc ccgccggtac gcaggacggc aagcagttca aggtaagggg cgcgggagcg
      301 ccgagggtga ggaacgatgg cgcgcagaag ggaaacctca tcgtgcgggt aggagtggta
      361 gtgccgaaga agctcaagcg gcgggaacgc gagatcctgg aggccctcgc cgaagaacgg
      421 gatgaggatg tcagggagga tctattcaag aaggtgggat cgtgagcctc agaaagcgtc
      481 cggtgtacat gatcgggata gcttcagagc ttatcggggt gcatccgcag acgctcagga
      541 tgtacgagca gaagggtctt ctccgcccgc gtaagagtat aaagaacacc aggctctatt
      601 cgcaggagga cgtggacctc ggccgctaca tacagaggct gacccaggag atggggatga
      661 acctcgccgg tgttggcaag gtgatcgacc tcgagcgcca ggtagcgaag ttgaaagccg
      721 aagtgaatgc tctgcgcgaa gagctggacc gcaaggaacg ggagcacagc cgcctgatct
      781 ccgaagtcca tcagagctac aagagagagc tggtcctgat gccccgtggc gagcttgcgc
      841 ggagcgggag gaaggatcgc tgatggccga gcctatgcac tgctaccggc atccgaaaag
      901 ggagacgcga gtctcatgcg ccacgtgcgg gcggcccatt tgcaccgagt gcatggtggc
      961 caccgacgtc gggataaagt gccccgacga tgcaaggctg ccgaggggag cgcgggccgg
     1021 cgtcatgaag acggatcagg tcgtaaagag tatcctcgcc ggcgccgggg tcgctctcgc
     1081 cggtatcccc gttgcgtacg tgctcttcct cctcccattc acctggttga tctcggccgc
     1141 ggccggttac ggggccggcg ttctgatcaa ccgcgccggg ggccgtaacg gcgggccact
     1201 ggcgatcgtc atctccgtcc tggccacggc cgtaccgttc ctggtcttgc tggcgccgga
     1261 tctcctcgcg ggcgcactca acccgcggcc actgatcgcg atggtattcg ccgttatcgc
     1321 cgccggcata gccaaccgtc ggatatagag ccctcggctt tcaacagcct cgtatgggac
     1381 aagccccgct cgcaatatga gcgctacctc tcggagcagc aacaaggtat gcgcacgatc
     1441 ttcctcgcag aagacttcgc cgggtactcg acggtcgtat gcaccccttc ctggccgcgg
     1501 gcatcccgga gatccaagac ttcaaagtgc tgccgcgctt cactctccct tctaacgggt
     1561 cgaccactgc tccagagtgg aaaacccctg tggataactc ccagaacccc accaataaag
     1621 cgaaaaaccg ggctcctcag gttgcggtag agatctttac tttcgtgaaa caaagttaca
     1681 cttaactcta agataaaatt aaacctgaca atgaggtaaa gcatcgaaaa atactttcag
     1741 aaagcctagc gtaggctgtg agagcgtgct agggttggag ccatgagaga ggacatgcag
     1801 tacataagcg gagttttgga cgagctggag gcgatctttc aggatgcatc cggtgtaccg
     1861 atgagcaaag gccgggctgt tgtggatcgg tctgatctgc ttgtgatgct ggacgagctg
     1921 aggggatctc taccccctga gctggctgaa gccgaggcgc ttcgccggga atgcggggcc
     1981 actgtcgctg cggcggagga ggaggcccag cgcataatcg aggaagccca ccagcgcgct
     2041 gatgcgctgg tccccgagac ggagctcctc cggcgggccg agcgtcgggc cggagaggtc
     2101 atagatgggg cggagcgcta cgccgaggag gtgtccggcg gttcggaggt ttaccgggac
     2161 cgcgtcatgg gtcagttgga gaactggttc caggactcgc tcatctccgt ggaggagtcc
     2221 aggcaggagt tgggcggtgt gcccgtgcgt cggcctgcgc cccgctccga ggtcgtcgac
     2281 gaagagaccg acggccaggg ctggcgggcg agctcggcct agtttgcaaa gaggtcaaga
     2341 cctctttgca agcatttcag cacgctcagc ccttcgggct tcgcttgtcg gctagtcagc
     2401 attctgtcga agtggcgaga ccatcacacg gcttcgccgc tctgtcaagg acgtaagttt
     2461 ggtccatctt cgaccggttg tcatctcttc tgttcgccta ccgcattctt gctgactagc
     2521 tgaagtgctg agagccgccg caggcggcgt gctgactagc tctcttcttg cgggaccatg
     2581 agttcgctga gggcttcgag cctggcattg aggccgtcga gtgaggcgtt gaggtctttg
     2641 gtccgggcag ggttctcgct atctatggag atccggacga ccttggccct gaggtccaga
     2701 agctcctggt atgtatcgga gatctcggcg tctgcggcgc ttatcctgtt ttcgagctcg
     2761 cgcaggttct cctgtcttgc ggtggtcggc gtggcagagg cggtatcctg gccgaggtct
     2821 cgtgcgactt cggcggccct ctctctacct tgggccacgt ccacgagcct gtccgctgcg
     2881 gcgtgcagcc tggggaccgc atcgtcgagg accgacctgg ttacgtcgtc tgaggtctcg
     2941 atggcggcga cgaccttgcg gtagacaccg agggccgcct ccaccctctg gcgggtctct
     3001 ccgcggggga gccagcgggc gcggcgctcg ggagagagtt cggtgctctc tacggcgttc
     3061 ggcgatgtct tgggaacctc gcctctaccg aggaccttgc gctggaagat agggtctctc
     3121 gccgccagga agaccagcgc agcgtaggtc aggatggtca agggcaccaa ccaccacgca
     3181 agggtggtca ggaaggcgcc cgccccgatc acgagcatcg tgacgttgat ggggcgcagc
     3241 gccgccgccc gcagcatcct gtttcgctct ccgggcgcga gggtagcgtt ctcccggtcc
     3301 accggaggac ccgccccgga gttccggcgt tcaggcaccg tgatccgccg ggagtatcaa
     3361 acgtaccttt cctccgatct cgagcgcgag cgcctgggcc gcgctacctt tgtttatggc
     3421 gaggctcagg cgccagtggg agtcggggac gagcaccagt tcgccggcct tggccgaccc
     3481 gaatgtctcg acgtatcgaa ccgccatccc gccgtcaccg gcgtctactt tcagggcgtc
     3541 accatactcg agccccgact cctcctgcat gatggagagt ctggcgttgc tgaagcggtc
     3601 gacggagatg atgtgggtaa cgagaccagc tccctcatct atcctcacgt tcgccggtcc
     3661 gagacccacc agtgaggaag g
//
LOCUS       DATMVF010000281         5496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_685409, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000281 DATMVF010000000
VERSION     DATMVF010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685409"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..738)
                     /locus_tag="VN178_14545"
     CDS             complement(133..738)
                     /locus_tag="VN178_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS82236.1"
                     /translation="MAERGRQYLILADEDLISLVEAADAEAFATLYDRHSRAAFSLAY
                     RMMGERQASEDLTQDAFLKVWRGASSYRADRGSVRTWILSIVHNRGIDQIRSQASRRR
                     TQERVEASAPRSQPSEAFAEAWRNSQRDQVREALNTLPSEQLKILELAYFSGYTHVEI
                     SDLLGLPLGTVKGRMRSGVKKIRDYFDYPTFPRRQEARPGD"
     gene            922..1746
                     /locus_tag="VN178_14550"
     CDS             922..1746
                     /locus_tag="VN178_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS82237.1"
                     /translation="MRLSVLSSGSSGNATYIETESGGLLVDAGLPPRRINALLAQIGR
                     NLTDVYAVLLTHGHTDHTCGVPALVRDHDIEVFAAPGVGESLGAFTVASGEPCSVEDL
                     GVTFFEVPHDSMTYGVRVSDGSRAVAMATDLGEAGIDVLRSMSGAEAVVLEANHDPDW
                     LSRGPYSASLKRRIRSRHGHLSNRQAAAAAVALAPHGLKDLVLAHLSKTNNSPTRACG
                     TVDRALREAGHGSVRVRAAIAGHPTPWIEVGAPLVVSEHVYHYGERGAAGQLFGID"
     gene            1904..2377
                     /locus_tag="VN178_14555"
     CDS             1904..2377
                     /locus_tag="VN178_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWS82238.1"
                     /translation="MISRCTLVAVGKPKDWAAEASEDYAKRLRRYFPVTIVEVAEEDM
                     NRLSPKEVLAAEGKRILKRLPTEAYVISLDREKGKQHPSEKLALKLEALGASGRSHVA
                     FVIGGPLGLSPEVLQRSNALWSFGSITLPHALARVVLLEQLYRAAKIHRNEKYHW"
     gene            2481..2702
                     /locus_tag="VN178_14560"
     CDS             2481..2702
                     /locus_tag="VN178_14560"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HWS82239.1"
                     /translation="MRVQIDRFEDNGWAVLLPYPDGRTTFDVPRELLPEEVSAGDIFE
                     VRFEYDREETLRIAEENRRLLDELLGGSE"
     gene            2699..4336
                     /gene="argS"
                     /locus_tag="VN178_14565"
     CDS             2699..4336
                     /gene="argS"
                     /locus_tag="VN178_14565"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWS82240.1"
                     /translation="MSFEERLAGAVREAVYRTYGVELNGIHVERPNDPAHGDFATNVA
                     LANARVLRRNPREVAENLVEALDAPFVREAEVAGPGFVNFRLSPEALWDEVGSLLREG
                     ELYGRREPSGDPVLLEFVSVNPTGPMHVGHGRQAAYGDSLARIMETAGRNVSREYYFN
                     DGGNQIRLFGESVAARYARLYGEIWPVSGSETAYMGDYTEEIAEDIAREHDRRLLDVD
                     REEALREITAFATRWCMDDIRRTLARVRVRFDSFFNEKSLYESGAVEEIVRELVGGDH
                     VYESEGALWLATSKFGDDKDRVLVKSDGSYTYMAPDLAYHRDKWERGFRTAVDVLGAD
                     HAGYPPRIRAGLVALGLPREFLDVELVRLVKLVREGEQVKFSKRAGNVVGFDELLDEV
                     GEDVARYFYVRSSHKTEMNFDLDLAIKHSDENPVFYVQYAHARISSIFERAGISPEEV
                     SEIPAGDLALEERRLLLELLDFPRVIQNAAQRREVHPIPTYLETLATRFHQFYTVHRV
                     LVEDADVRARRLALCAATKSVLRSGLDLVGVNAPEKM"
     gene            4344..5171
                     /locus_tag="VN178_14570"
     CDS             4344..5171
                     /locus_tag="VN178_14570"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HWS82241.1"
                     /translation="MAADGDIRFVTYLSPSIPRALFEALADHVQRTLGHEEVSLRVES
                     KASGPQNRGECSSFAEEADVAFMCAPSFVWLRDLRPPPAELLGVLPVFDDERNQGKPV
                     YFCDVVVRNDAPIQAFSGLEGGTWAYNDACSLSGYYSLFDKLAESSADESFFDNIFCS
                     GSHLNSIEAVLNGRADAAAIDSNVLRIRFREAPTLRKELRVVESWGPYPIQPVVVSSA
                     LRPDLKQRLRSAFLSTEQDQQTRRTLQQFGLSRFVAADQRAYSFDAHKDLAVLLAAR"
     gene            5245..>5496
                     /locus_tag="VN178_14575"
     CDS             5245..>5496
                     /locus_tag="VN178_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWS82242.1"
                     /translation="MGTQGRMVICQISDIHCGSPYFIPDLLERSILEINDLDPTVVVV
                     SGDLTNAGYRQEFEEAADYIRRIQCERVMVIPGNHDSRNV"
BASE COUNT         1086 a         1793 c         1654 g          963 t
ORIGIN      
        1 caacttcaga gaatttccgc ttgacatttg gtccttcaga acccgctccc tcggaccgcc
       61 gcgaaccctt gagcatcttc ccgcccccgg aagcagtgcg tcctctcgcc gatggtggct
      121 aaggacgcgc ttctagtcac ccggcctcgc ctcctggcgt cgaggaaacg tgggatagtc
      181 gaagtagtcc ctgatctttt tcactcccga acgcatcctg cccttgaccg tcccgagagg
      241 caggccgaga aggtcggata tctccacatg cgtgtacccg gaaaagtacg ccagctctag
      301 tatcttgagc tgctctgacg ggagggtgtt gagagcctcc ctgacctggt cgcgctgtga
      361 gttcctccag gcctccgcga aggcctcgct cggttgggag cgtggggccg aggcttcgac
      421 tctttcttgc gtcctgcgcc ggctagcctg ggaacggatc tggtctatac cgcggttgtg
      481 gactatggag aggatccagg ttcgcacgct gccgcgatcc gcgcgatagc tgctggcgcc
      541 gcgccagacc ttcaagaacg cgtcttgggt caggtcttcg gaggcctggc gttcccccat
      601 catcctgtaa gccagggaga aggctgcgcg gctgtggcga tcatacagcg tcgcaaacgc
      661 ctcggcatca gccgcctcga caagggagat caaatcctca tcagcgagga tcaggtactg
      721 tcttcccctt tcagccatac cctgattcta cggcaatacg gctgctccgg atcagcccgt
      781 gcccggacgt cggatccgaa tcacgagaag cgcctgatcg tccaggtcga agtcatctgt
      841 acgagcacct gaacgcagcg tgctaagatc tgatcgggcc tcgttcccgc agatcaaacg
      901 cccaacctga agaggactga cttgcggctg agtgtacttt cgagcggaag ctctggcaat
      961 gcgacctaca tagagacgga atcgggcggc ctcctcgtcg acgccggact cccgccacgg
     1021 cggataaacg cccttctggc ccaaatcggc agaaacctta ccgacgtata cgccgtcctg
     1081 ctgacccacg gacacaccga ccacacttgc ggcgtccctg ctctggtgcg cgaccacgat
     1141 atcgaggtct tcgccgcgcc cggtgtcggc gagtccctcg gcgcgtttac ggtagcctcg
     1201 ggagagccgt gctccgtcga agaccttggg gtgacgttct tcgaggtccc gcacgactcc
     1261 atgacctacg gggtcagggt atccgacggc agtagagccg tggcgatggc gacggacctc
     1321 ggggaggccg gaatcgatgt actgcggagc atgagtgggg ccgaggcggt ggtcctcgaa
     1381 gcgaaccacg atccagactg gctcagtcgg ggcccatact cggcgagcct gaagcgccgg
     1441 atccgctccc ggcacggcca cctctcgaac cggcaagcgg cagccgccgc cgtggcgctg
     1501 gccccgcacg gtctcaaaga cctcgtcctc gcccacctct ccaagaccaa caactctccg
     1561 acccgggcat gtggcaccgt agaccgggcg ctcagggaag cgggccacgg ctccgtcagg
     1621 gttcgggctg cgatagccgg ccatccaaca ccctggatcg aagtaggagc cccgctggtg
     1681 gtctccgaac acgtctacca ctacggcgag cggggggctg caggacagct cttcggcatc
     1741 gattgatacg gaaaacgcag gaccactttc ccgcggtgca caaggtcagc gagcacaatg
     1801 cccacccctc tgctcccagc ataaccgtca ttcccgcgaa agcgggaatc cactacgaca
     1861 gaaggttcgc acctcctacc acaactgact tacccaattg cgcatgataa gccgctgcac
     1921 cctcgtcgcc gtcggaaaac caaaagactg ggccgccgaa gcctccgaag actacgcaaa
     1981 acgactgcgc cgctacttcc ccgtaaccat cgtcgaggtc gccgaagaag acatgaaccg
     2041 cctctccccc aaagaagtgc tagccgcaga aggaaagcgc atcctaaagc gcctccctac
     2101 agaggcgtac gtgatctccc tcgacaggga gaaaggtaag cagcacccat cagagaaact
     2161 cgccctaaag ctcgaagccc tcggcgcctc gggccgcagt cacgtcgcct tcgtgatcgg
     2221 tggcccgctc ggcctctccc ccgaagtact gcaaagatcc aacgccctgt ggtccttcgg
     2281 cagcatcaca ctcccacatg ccctcgccag ggtcgtcctc ctcgaacaac tctaccgggc
     2341 agcaaagata caccgcaacg agaaatacca ctggtagcgt cgcttcactc cgcagctatc
     2401 agccgtcagc agggctcggc ttggcgatgc caaaaagctg aaagctgact gctgaaagct
     2461 gactgctaaa atacacatat atgcgcgtgc agatagacag gttcgaggac aacggttggg
     2521 ccgtgctcct gccctacccc gatgggcgca cgaccttcga cgtgccgcgc gagttgctac
     2581 cggaagaggt ctccgctggc gatatcttcg aggtccgctt cgagtacgac agggaggaga
     2641 ccctccggat cgccgaggag aaccggcggc ttttggacga gcttctcggg ggttcggaat
     2701 gagcttcgag gagcggctgg ctggcgccgt gcgggaggcc gtctacagaa cctacggggt
     2761 ggagttgaac ggcatccacg tcgagcggcc gaacgacccc gcccacggcg acttcgcgac
     2821 caacgtggcc ctcgccaatg ccagggtttt gcggcggaac ccgagggagg tagccgagaa
     2881 tctggtggaa gccctggacg ccccattcgt gagggaggcc gaggtcgcag gaccaggctt
     2941 cgtcaacttc aggctctctc ccgaagcgct ctgggatgag gttggttccc tgctacgtga
     3001 gggtgaactg tacggtaggc gagagccttc gggggacccg gtattgctcg agttcgtgag
     3061 cgtcaacccg acgggaccaa tgcacgtggg gcacggacgt caggcggctt acggggactc
     3121 gctggccagg atcatggaga cggccggcag gaacgtctca cgtgagtact acttcaacga
     3181 cggagggaac cagatccggc tcttcggcga gtcggtcgcc gcgcgctacg ccaggctcta
     3241 cggggagata tggcccgtct ccggctcgga aacggcttac atgggcgatt acacggagga
     3301 gatagcggag gacatcgcca gagagcacga ccgccgcctg ctggatgtgg accgcgagga
     3361 ggccctgcgt gagatcaccg ctttcgcgac ccgttggtgc atggacgaca tcaggcgcac
     3421 cctggcccgc gtgcgggtcc gtttcgatag cttcttcaac gagaagagcc tctacgaatc
     3481 gggcgccgta gaggagatcg tccgcgagct tgtggggggc gaccacgtgt acgagagcga
     3541 aggcgccctg tggctggcga cctcgaagtt cggcgacgac aaggaccggg tgctcgtcaa
     3601 gagcgacggt tcgtacacct acatggcccc cgatctcgcc taccaccgcg acaagtggga
     3661 gcgtggtttc cggacggctg tggacgtgct cggcgccgac cacgccggct acccgccgcg
     3721 aatcagggcc ggtctcgtcg cgctcggatt gcctcgcgag ttcctcgacg tggagctggt
     3781 gcggctcgtg aagctcgtgc gcgagggaga gcaggtcaag ttcagcaagc gggccggcaa
     3841 cgtcgtgggc ttcgacgagc tgctcgacga ggtgggggag gacgtggcgc gctacttcta
     3901 cgtgcgctct tcccacaaga ccgagatgaa cttcgacctg gatctcgcca taaagcactc
     3961 cgacgagaac cccgtcttct acgtgcagta cgcccacgcc cgcatatcca gcatcttcga
     4021 acgggccgga attagcccgg aggaagtgag cgagatcccg gcaggagacc tcgccctcga
     4081 agagcgccga ctcctgctcg agctcctcga cttcccccgc gtcatacaga acgcagccca
     4141 aaggcgcgag gtgcatccca tcccaaccta cctcgagacc ctcgccacgc gattccacca
     4201 gttctatacc gtccaccgcg tcctggtcga agacgccgac gtgcgcgccc ggcgcctcgc
     4261 cctttgcgcc gccacaaagt ccgtgctccg cagcggcctc gacctggtag gggtgaacgc
     4321 gcccgagaag atgtagggga acgatggccg cagacgggga catccgcttc gttacctacc
     4381 tctccccaag catcccacga gccctcttcg aggcgctggc ggatcacgtg cagcgcaccc
     4441 tcggacacga agaggtgtcc ctgagagtag agtctaaggc ctcgggaccg cagaaccggg
     4501 gcgaatgctc ctcctttgct gaagaagccg acgtggcgtt catgtgtgcg ccgtctttcg
     4561 tctggttgcg cgacctgcgc ccgccacccg cggaactgct cggcgtactg cccgtgttcg
     4621 acgacgaaag aaaccagggg aagccggtct atttctgtga cgtcgtggtg cgcaacgacg
     4681 ctcccatcca ggctttctcc ggactcgaag gaggcacctg ggcgtacaac gacgcgtgct
     4741 cgctgagcgg ctactacagc ctgtttgaca agctggcgga gtcgagcgcg gacgagagct
     4801 tcttcgataa catcttttgc tcaggatccc acctgaactc catagaggct gttctgaatg
     4861 gtagggccga cgccgcagcg atcgactcca acgtcttgcg gatacgattc cgagaggcgc
     4921 ccaccctccg taaagagttg cgcgtcgtcg aatcctgggg cccctacccg atacagcccg
     4981 tcgtagtcag ctcggcactc cgtccagacc tgaaacagcg acttcgctcc gcattcctct
     5041 ccaccgaaca ggaccagcaa acgcgccgaa ccctgcaaca gttcggcctc agccgcttcg
     5101 tcgccgctga ccaaagagcc tactccttcg acgcccacaa agacctcgcc gtactgctcg
     5161 ctgccaggta gtccgaaatc tgtagcctaa tgcctctttg ctatactctc ggcggcgtct
     5221 ctatcgaata gaatcggaga gcgaatgggc acgcagggca ggatggttat ctgtcagatc
     5281 agcgacatcc actgcggaag tccctacttc ataccggact tgctcgagcg ttcgatcctc
     5341 gagataaacg acctcgaccc cacggtcgtc gtggtctcgg gtgacctgac caacgcgggt
     5401 taccgccagg agttcgagga ggcggccgac tacatccgtc gcatccagtg cgagcgggtc
     5461 atggtgatac cgggcaacca cgattcgcgc aacgtc
//
LOCUS       DATMVF010000282         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_504674, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000282 DATMVF010000000
VERSION     DATMVF010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504674"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..1939)
                     /locus_tag="VN178_14580"
     CDS             complement(536..1939)
                     /locus_tag="VN178_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWS82243.1"
                     /translation="MADRQETLGSFRGKLLEEAVVQEFAGNLRGELLRPEDDGYDTAR
                     AVFNGMIDRHPALIARCAGVADVIRGVDFARTHDLPLSVRGGGHSVAGNAVCDGGVML
                     DLSPMKGMRVDPVSQTAQAQAGLTLGEFDLETQQFGLATTLGVVSMTGIAGLTLGGGL
                     GWLNGKYGLACDNLISADVVTADGRMLTASEEENEDLFWCIRGGSGNFGVVTSFEYRL
                     HPVGPVLAGGLSYPLAEAHEVLRFYHAFASECPDELSTVASLGISPDGSPEVYVSVCY
                     CGAIEEGERVLRPLRTFGSPMEDSIQPIAYRALQSAPDAEFPFGRQYYWKSGYLKYLS
                     DEAIEVMVRFVREIPSPAARVSLQQMHGFASRVDPGATSFPHRDEHYDLVILSEWTDS
                     TDTAKNIEWTRAFYDAMKPFSERGVYVNNLGEEGEDRVRAAYGRNYDRLAALKNKYDP
                     TNLFRLNQNIKPIETSS"
     gene            complement(2072..>2810)
                     /locus_tag="VN178_14585"
     CDS             complement(2072..>2810)
                     /locus_tag="VN178_14585"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWS82244.1"
                     /translation="QLIVPGFYARMAAATGLIGFACANAVPMVAPPGGRTPTLGTNPF
                     AYAIPAGRYPPVVLDVATTTGAAFEIRLAAQRNRPVPEGMILDGEGRPTTDPNEFIRG
                     GLMAPLGSPAAPHKGFGLGLLFDTLAGVLTGAAFARNFPSEPATAGNAFFWALDVEAF
                     LPREEFLGRMEAQIDQVKSGERLAGVDELFLPGERSHRRYHELTTRGMVPLSGAAWET
                     LTKACASLSIPPPALAPEPRRTDNGSA"
BASE COUNT          503 a          895 c          915 g          497 t
ORIGIN      
        1 aagatcgggg cccgcaccaa ggatgtcctc atcgaggcga tgggtctagc attggcggcc
       61 gtgagcgccg aagacgcgaa agggtacttt gctcactgcg gataccgctc cccggcgcag
      121 caactatgaa aggcgctgtc agaaccagaa tgaggtcggc agcgtttggt cgctcgacag
      181 tcggctgggc tacgactagg aggaggatca caaggaggcg ccgaagcggg cttcggagcg
      241 cctcaatgcg cgggcgagac cgggcccgcg agacgatcga cgagaacggg ggagtcaaag
      301 gttcgtgcct cccgtagaac ccgggcgcgg gcatccgaaa gggagaggcg cgtgtcgcgg
      361 tcgttcgcgg agtggtgtgc agtcttcgac ctgctcggcg cggtgggcat cctcgccgcg
      421 gctatcctgt agccgcggtc cgtgccgcct gaccattcta tgcctcactt tctccttggg
      481 aggatacgac ggaacccgta gatccaggtc ccgtttccgc aaggaaccgg gtagcttagg
      541 acgacgtctc gattggcttg atgttctgat tgagacgaaa gaggttcgtc ggatcgtact
      601 tgttcttcag ggctgcgagg cggtcgtagt ttcggccgta agctgccctt actcggtctt
      661 ctccctcctc tcccaagttg ttgacgtaga cgccccgctc ggagaaaggc ttcatcgcat
      721 catagaacgc gcgcgtccac tcgatgttct tcgccgtgtc tgtcgaatcg gtccactccg
      781 agagtatgac gagatcgtaa tgctcatccc ggtgcgggaa agacgtggcg cccgggtcca
      841 cgcggctggc aaacccgtgc atctgctgga ggctgaccct tgcggcaggc gagggtatct
      901 ccctcacgaa ccggaccatg acctcgatcg cctcgtcgct gaggtacttg aggtagcccg
      961 acttccagta atactgccga ccgaacggga actccgcgtc gggcgcgctc tggagggctc
     1021 ggtacgctat cggctggatg ctatcttcca ttggtgaacc gaatgtgcgc aaagggcgca
     1081 gcactcgttc tccctcctcg atcgctccgc agtagcagac cgaaacgtag acctcgggac
     1141 tcccgtccgg agagatgccc agcgaagcga cggtggacag ctcgtcgggg cactcactcg
     1201 cgaaggcgtg gtagaagcgg aggacctcat gggcctccgc cagcgggtac gagagtccgc
     1261 ctgccagcac cggacccacc ggatgcagcc ggtattcgaa cgaggtgacc acgccaaagt
     1321 tcccactgcc gcccctgatg caccagaaga ggtcttcgtt ctcctcctcg ctagccgtca
     1381 gcatccgacc atccgcggtc actacgtcgg ccgagatgag attgtcgcag gcgaggccgt
     1441 atttaccatt gagccacccg aggccacccc cgagggtgag ccccgcgata ccagtcatcg
     1501 agaccacccc gagagtggtc gccagcccga actgctgcgt ctcgagatcg aactcgccga
     1561 gcgtgaggcc tgcttgggct tgcgcggttt ggctcactgg atcgacccgc atgcccttca
     1621 tgggtgacag gtcgagcatc accccaccat cgcacacggc attgcccgcg acgctgtgtc
     1681 caccaccgcg aaccgagagc ggcaggtcgt gtgtccgggc gaaatcgact ccccggatca
     1741 catcggccac acccgcgcag cgggcgatca aagctgggtg ccggtcgatc atgccgttga
     1801 agaccgcgcg ggcagtgtcg tagccgtcgt cttccgggcg cagtagctcg ccgcggagat
     1861 tgcccgcgaa ctcctgcacg accgcttctt cgaggagctt gcccctgaac gagcccaagg
     1921 tctcttgccg gtctgccatg atccatcacc ctttcctcgc ctttccacga ccccagcatg
     1981 ctaaggtgag cataactgcg catcctccaa acaaacgaac ggtaggcctc cggccagtcg
     2041 cgctcctctg cgaccgccgg ctcttgcggt attaggctga tccattatct gtccgtcgcg
     2101 gctcgggcgc aagggcaggc ggtgggatag acagggatgc gcaggccttc gtgagcgttt
     2161 cccaggccgc gcccgagagc gggaccatac cgcgagtggt tagctcgtgg taacggcggt
     2221 ggctacgttc gcccggcaag aacagttcat cgacgcctgc cagccgctcg cctgacttga
     2281 cctgatcgat ctgggcctcc atccgcccca gaaattcctc gcgcggtagg aatgcctcaa
     2341 cgtcgagcgc ccagaagaag gcgttgccgg ccgtggccgg ttcgctggga aagttgcggg
     2401 cgaaggctgc cccggtcaag acgccggcca gcgtatcgaa gagcagtcct aggccgaagc
     2461 ccttgtgggg tgcggccggg gacccgagtg gcgccatgag cccaccacgg atgaactcgt
     2521 tcgggtccgt agtcgggcgg ccttcgccgt cgaggatcat gccttcgggc acaggacggt
     2581 tgcgttgcgc cgcgagccgg atctcgaacg cggcgccggt ggtcgtggcc acgtcgagca
     2641 ccaccggtgg gtagcggccg gccgggatgg cataggcgaa cgggttggtg cccaaggtcg
     2701 gcgtgcggcc cccgggaggt gcgaccatcg ggacggcgtt ggcgcaagcg aagccgatca
     2761 ggccggtcgc ggccgccatc cgggcgtaga acccgggcac gataagttga
//
LOCUS       DATMVF010000283        35624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_589051, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000283 DATMVF010000000
VERSION     DATMVF010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 35624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35624
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589051"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VN178_14590"
     CDS             <1..283
                     /locus_tag="VN178_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82245.1"
                     /translation="MLRFHEGKEKAAPDLDAKKAWRPVTAGLRVWETEMSSEEVESFE
                     AAAGDLLDELGYPRAAPRPSPRFVEEVAGVRRAFTEDLRVQGHRIPERW"
     gene            280..1236
                     /locus_tag="VN178_14595"
     CDS             280..1236
                     /locus_tag="VN178_14595"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS82246.1"
                     /translation="MNPYVFIVGAARSGTTLLQRMVDAHPDIAVIHESHWIPRFYEER
                     IGLTADSTVTLELIPRLLDDRRFVKLGISQRDLEGLIPSDRTISYADYVARIFDLYGR
                     ARGKRLVGEKTPGYVRSIPTLHYLWPEAKFVHLIRDGRDVFLSMKSWKRVDRSVGRFA
                     AWRRDPVPSSALWWQWDVERGREAANLLGPELYHELRYERLVSKFAEELAKLCAFLGL
                     PYDEAMLGFHEGRTKTKPGLDAKRAWLPLTPGLRDWRTEMPAADVEIFEAAVGGLLDD
                     LGYERAFPNPSSEAIECASETRRRFVRELDERERRTPVNRRS"
     gene            1364..2104
                     /locus_tag="VN178_14600"
     CDS             1364..2104
                     /locus_tag="VN178_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWS82247.1"
                     /translation="MRKIAFINAKGGVAKTTCCVNIGAGLALGGYRTLLIDLDSQGQV
                     TKSLGVQAGEGLAELLQGDAGLKDVQIEARENLFVIAGGGALSGAKRLISRMDMRSEL
                     VLSEALEDLRGYDFVLLDTAPSWDVLNVNCLFYVDELLIPVSMEALALQGIGDFLGRV
                     DQVRRYREGLRVLSIIPTFCDGRVRKSAEILEQLERHFPDALWPCVRYNSKLSEAPGF
                     GQHIFEYSKRSNGAADYALLAERLAKGR"
     gene            2113..2751
                     /locus_tag="VN178_14605"
     CDS             2113..2751
                     /locus_tag="VN178_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82248.1"
                     /translation="MGKSRKVPKDLMGTILRESAGPPGGERNSPESVEPGMVSPASGG
                     EGTISWEDQPWLVGTTFNLSKQVGRELDRLRHDLLRQEDIHTSNSEIAEVALRIAIEG
                     ARERGRGSELVGRLLSGLGGRATYGAGGAGLARRSVDGSGRILETTYNEEGQVMDEEE
                     VGDVTDLPVETEYVDAQGRLLSLARDELGNTFEWVTDEELGTLEARIFRPSG"
     gene            2829..3371
                     /locus_tag="VN178_14610"
     CDS             2829..3371
                     /locus_tag="VN178_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82249.1"
                     /translation="MSIPRYYTVGAVAQDLRALKALDERLEDLGVPADSLLVLSRRKD
                     ERLVGVTLPGARTRRVESGPTRTQWFELGSTYLGVTAVSVLMGAVHPPTGIAVQAVIT
                     LATIIGLILYHRRPHLQKKLLTMGLPESFAGEWEAAFHDGFALALATVPSDLHDETQD
                     AFLEDSELGSPLAVDRRPVL"
     gene            3610..4305
                     /locus_tag="VN178_14615"
     CDS             3610..4305
                     /locus_tag="VN178_14615"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HWS82250.1"
                     /translation="MIIRERPDSFLLFGQHEHALVAGEFARRWRERPNPFESTVYAVA
                     NHDLAWREADRGVLWNEEKDRPYSFVDYPLDLKLPAQRRGIDLVEEHDPYAGCLCSMH
                     YARFLLDSVRPEEVEFREDEVGRQDRLEERMAENELENLERNFRLLRLCDGLSLYLCL
                     NEPGDGDGPPPYPGGFEFEGTRFVPVWEDGQTLRLDPNPFSEAFGIEIPYRIIGTDRR
                     QLGSDILELRVTC"
     gene            4361..5419
                     /locus_tag="VN178_14620"
     CDS             4361..5419
                     /locus_tag="VN178_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HWS82251.1"
                     /translation="MRDASYDFLKRLLSTPGPSGYEEAAAEVWREEAGKFAEVKGDRM
                     GNSFATIGAGGSPKVMLAGHIDEIGVMITHTEENGLLRFTGVGGWDSQVLVGQRIRLL
                     TKDGELVGVIGKKAIHVMEPDERKKVSEIKSLWIDVGAKDGEEAKGMVRVGDVGVIDQ
                     DLIELPNGRLASRSVDNRMGAFVVLEALRLLSEEEDLGAEVVAVACVQEEIGLYGARG
                     AAFGLDPDAAIAVDVTHATDTPGVSKNEHGDHSLGGGPVIKRASNISPIVSEGLIAAA
                     EGENISYSLEADSRSTGTDADAIQFARAGIATGLVSCPNRYMHSPNEVVDVEDVENCA
                     RVISAYIMTLSAETNFVR"
     gene            5433..6326
                     /locus_tag="VN178_14625"
     CDS             5433..6326
                     /locus_tag="VN178_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS82252.1"
                     /translation="MTTEEEPKRQASIGVEAALLLTVFFFGTNFTAVKIVVESVPPIL
                     FAATRFTLAGLLLLLFVPFLEPGSRLRRGDFLAVLGLGVVGVTLTQTVFTIGVSLTTA
                     ANTALVYSTAPVWGMLLGFALGLERPRPAGILGIGLSLLGVGFIVYGGLEFAGTSLAG
                     DALILAAAVFWGSYTVLSLSLLKRYSPVALAAYAMTLGGLAAFPLAALDPGGLDIGSL
                     DGLTWGAAAYSALCSSAFGFAAWGYGVSRVGANRVLIYQYLVTLTGVTTGIVLLGEDF
                     GLEQLVGAVVIICGVYLARKR"
     gene            complement(6451..7545)
                     /locus_tag="VN178_14630"
     CDS             complement(6451..7545)
                     /locus_tag="VN178_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWS82253.1"
                     /translation="MKVHTLRRTLLLPLVALTVLAFVLGVIYLDPDTGRTQTADPVES
                     ARASIPADEPVARVASVLSPSVVQINVSGIKQTPYGAQKQEGLGSGVIYRSDGYIITN
                     NHVVEGSRDVEVAFADGTTERGEVVGEDPTTDIAVVRVGRNDLPAADFAGADPIVGQM
                     AVAVGSPSGFESTVTSGIISGTGREVPAEYTGGTQASSLVDLIQTDAAISPGNSGGAL
                     ADREGRVIGINVAYLPPAQTGAENIGFAIPSETAISVADQLVENGEAVHPYVGVYLSD
                     LTKETASRFGSPVDSGALIEEVEPGSPADDAGIRRGDIITAAGSEEVRSSGDLLSALR
                     NYMPGDTVGLEILRNGEKITLQVDLAERRE"
     gene            7728..8417
                     /locus_tag="VN178_14635"
     CDS             7728..8417
                     /locus_tag="VN178_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS82254.1"
                     /translation="MRILVVEDEERLARLISRVLLEEGYAVETEANGRQALMRALADD
                     YDLLIVDWMLPDLSGVQLVKRLRAAEVGTPAIMLTARDQIEDRVEGLDAGADDYLPKP
                     FAFPELLARVRALARRPWAEERDGAILGAGDVTLDPVRHVVRHRGETVDLTAKEFALL
                     ATLLQRPGQVFTRPVLLDTVWGASPEVYANVVDLYISYLRKKLDGDGETSHIRTVRGV
                     GYTFEPQPGPR"
     gene            8417..9700
                     /locus_tag="VN178_14640"
     CDS             8417..9700
                     /locus_tag="VN178_14640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWS82255.1"
                     /translation="MFDHIRRRLTVGYVGILALTLLVFSVVVVFMFYLRASDQQDDLL
                     LQKAEDKANSVLSGKDRYSVVKATTDYDVAVIVLLPDGSTYGDLDETSFSLGLPYAYL
                     AQSAGQERRTIKGTVPGPGGAVRVMSIPVKRYGEFGSVVAVVQAAQSRQAVTETVERL
                     IFVLVLSGLGALVLAAAGGFFMSRRAMRPVQEAFGRQRTFIADASHELKTPLTLIRAD
                     AEVLSRGIDDPDKATDNRELVDDLLGETDRMSAVLSDLLLLARLDAQKVSVSREPFDL
                     ALVISETSERFAVRARTEGKNLEVHHSGKLPARGDATRTGQSLAALLDNALRFTPPGG
                     TITVASRITDKHVEAIVTDTGPGIAPESLDRIFERFYRDDAHSAARSRGPSGGGTGLG
                     LAIARDLARAQGGEISAENAEGGGAKFTLSLPTGY"
     gene            complement(10020..11204)
                     /gene="moeB"
                     /locus_tag="VN178_14645"
     CDS             complement(10020..11204)
                     /gene="moeB"
                     /locus_tag="VN178_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWS82256.1"
                     /translation="MQTRQPLVESPNGAHELSNQEIARYSRHLIMPEVALEGQKQLKA
                     AKVLTVGTGGLGSPLALYLAAAGVGTIGIVDFDVVDESNLQRQIIHGTSDVGRPKVQS
                     AYEKLEDINPNVEVRVHEEALTSENALEIFEDYDVIVDGTDNFPTRYLVNDACVLLGK
                     PNVYGSIFRFEGQASVFYAEEGPCYRCLYPEPPPPGLVPSCAEGGVLGVLPGAIGTIQ
                     ATETVKLILGIGEPLIGRLLLYDALGMRFREMKLRKDPDCPVCGENPTVTELIDYQEF
                     CGIPQAETTERENGVPEITVGELKQKMDHGEDINVLDVREPHEYEVANIGVKLIPLGE
                     LPQRLVELDQDEYFVVHCKTGGRSAKAVKLLQDAGFQNVYNVKGGITAWSEEIDPSVP
                     KY"
     gene            complement(11256..11549)
                     /locus_tag="VN178_14650"
     CDS             complement(11256..11549)
                     /locus_tag="VN178_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWS82257.1"
                     /translation="MPKVKIPTPLRQYTGGNAEVEVGGATAGEALGNLASEHPGLRQH
                     LYTGDGKLRSFVNVYKGDEDIRYLEGQDTGVADGDEISIVPSIAGGSAEGRCR"
     gene            complement(11562..11981)
                     /locus_tag="VN178_14655"
     CDS             complement(11562..11981)
                     /locus_tag="VN178_14655"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWS82258.1"
                     /translation="MALKIGAGDLGRIHDHAREAYPEECAGALVGIDSGGIKVVVDVW
                     RAENTHEEERSRRFLIEPLEVKKFEERAEEQEMGLLGFYHSHPDHPAEPSEYDREHAW
                     PGYSYVIASLGAENVEDMRSWTLRDDRSGYDEETIVG"
     gene            complement(11982..12905)
                     /locus_tag="VN178_14660"
     CDS             complement(11982..12905)
                     /locus_tag="VN178_14660"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWS82259.1"
                     /translation="MITRINERAGTNVTELVGGTPLLELPGISAEIPGVDILGKAEWY
                     TPGGSVKDRPALWMIRDGEKTGSLTPEKTILDATSGNTGIAYAWIGAALGYRVRLCMP
                     KNASEERKKILRAYGVDFVLTDPDEGSDGAIREARRLYAEDPERYFYPDQYSNPANPR
                     SHYESTAPEIWEQTNGEITHFVAGLGTSGTFVGTATRLKEYNPAIEVISFEPDSAFHG
                     LEGMKHMESAIVPAIYDPTIADQNRAARTEDAYEMVKRVAREEGILIGLSAGAAVATA
                     LGVAREIESGTVVTILCDGADKYLSENFWEE"
     gene            complement(13069..13791)
                     /locus_tag="VN178_14665"
     CDS             complement(13069..13791)
                     /locus_tag="VN178_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HWS82260.1"
                     /translation="MTETRTRQQQSQVEKLPAQYINYVFFGLDPFWRRLPDEERARGK
                     REFLEAAQMHSGEMLLRTFSLMGLRADADFMLWRIGYDLDAFESMTGALLKTGLGKYL
                     RITYSYFALSRRSIYVGEHTPGFENRTYIVPGEGEYLFVYPFVKTRSWYALPMEERQR
                     MMNEHMQIGRKHYPVKNNTGYCFGIDDYEFILSFEAESPEKFQDLMMELRESEASSYT
                     ELDTPIFTCRRRTLPEILDTLG"
     gene            complement(13887..14864)
                     /locus_tag="VN178_14670"
     CDS             complement(13887..14864)
                     /locus_tag="VN178_14670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS82261.1"
                     /translation="MKYRTLGGTDIAVSEVGFGVWTVSTGWWGEVDDERSVRLLREAR
                     ERGINYFDTADTYGSGKGETLLADAFGHVRDEVVISTKIGYDFYNHTARRGQQERPQD
                     WSEGFIRFALEQSLKRLDTDYIDLLQLHNTKMDAIEHDGLFALMDEFRNEGKIRAYGI
                     ALGPKIGWIEEGVRAMREREIEGVQMIYNLLEQDPGRGLIEAARETDTSLIVRVPHSS
                     GMLEGHYDENTTFAKNDHRRHRPKEWLTSGLKKVEQLSFLTESGGRTLGQAALKFVLA
                     SPEVASTLPNIYGKEQIEEFAATSETPDLTSEELSRVAELYENDFGLEK"
     gene            14999..15763
                     /locus_tag="VN178_14675"
     CDS             14999..15763
                     /locus_tag="VN178_14675"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HWS82262.1"
                     /translation="MIARYAGGESGARSAARSGSVVVVVDAFRASTTIAVLVSKGARV
                     VPVTSVEEAASAETDFRIGERGSAKVAGFDFGNSPTEILGSKMPPGSSVALSTTNGTR
                     VLEAARGASTIVTGAFVNAGMLAEELAGVAHDGAEVVVVGCGWEGRRASEDEAAAGAI
                     LYRLYSRGVELDGRARSVVEGYLSRPVGALRNNSAARRLKRLGYEGDLEFCLAEDTVP
                     VVPRLVNDAFAGWALGSRWPRRSSARGPTQKAEISS"
     gene            15860..16060
                     /locus_tag="VN178_14680"
     CDS             15860..16060
                     /locus_tag="VN178_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82263.1"
                     /translation="MTMIITHEKTREVTREDVDRAIDVIERHLEKEARTRNLLDEQAI
                     MRPKDLCKQQGLDMVAELRGMM"
     gene            complement(16431..16520)
                     /locus_tag="VN178_14685"
     tRNA            complement(16431..16520)
                     /locus_tag="VN178_14685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16484..16486),aa:Ser,seq:gct)
     gene            complement(16574..17587)
                     /locus_tag="VN178_14690"
     CDS             complement(16574..17587)
                     /locus_tag="VN178_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWS82264.1"
                     /translation="MESDTAETKLRDVAQRPARGSLGNFFGDLRDLLRYETLKATIKE
                     FMRDDALGLGAQLAFYLILAIFPFILFCVAILDAFSSASPQFATELFVYLERLLPAQV
                     FGLIQTYTENTLRNADTAPGLLSVGILGTIWAASGAFAALMNALNRAYDVQETRPFWK
                     VRGISILMTFGLSALILIGVLLLIAGPSIGRTLAEVLTLGDEFMFVWNILRWPAALLF
                     MVVTVALLFYFAPDANQPFRWITPGGVVGILLWVLASVAFNLYLSSDFNTYNRTYGSI
                     GTVIILLLYLYISSLTILFGATLNATLVRIKEEVSGEQIIDAEPANDKPDIIDGESND
                     NER"
     gene            17818..18375
                     /locus_tag="VN178_14695"
     CDS             17818..18375
                     /locus_tag="VN178_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS82265.1"
                     /translation="MDRVKDEVLVQRVASGDERAFSGLYDRYAGLVYGTGLRYLGDRG
                     TAEELVQDVFTSVWKSAGGFDPARASFATWVYRITRNRATDLIRRRKARVRTVEGEPL
                     FEPGETDPSGDLSRNFDVAAALSRLTPVHREVLVLAYFHGFSQREISDRTATPLGTVK
                     SRTTAALRALRETMGFDEDRFSADE"
     gene            18368..19024
                     /locus_tag="VN178_14700"
     CDS             18368..19024
                     /locus_tag="VN178_14700"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWS82266.1"
                     /translation="MSEHPDTRDLLGPYVMGTLEPYENREVEDHLQGCASCREEAQDL
                     RLAHERLADLAYGTTAPPEDLEARVLAGIPRRRAGRRVPSWVAAVAAAFCVLAVITAF
                     NLLEDQAAATLNPTGRAPDAGGEVSLRGTGENTELRLEAWGLPPCKSEQYYELWLVEG
                     DERVSAGTFTVGESGRVDVTLNAPNFAASYPRVGVTAEYDKDPRASDAKMLSGELHDL
                     "
     gene            complement(19145..20632)
                     /locus_tag="VN178_14705"
     CDS             complement(19145..20632)
                     /locus_tag="VN178_14705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWS82267.1"
                     /translation="MLRRPDASREDDERFRFHADNARDLIYRLRFHPTPGFEYVSPSS
                     TAMTGYSPEEHYADPDLGLKRVHPEDRHLLQNSMHSPEEPLVLRWYHKDGHLIWTEQR
                     NRPIYDEAGNVIAIEGIARDVTERKLAEEALRQNEEQFRLLAEKTSDLVCLHEPDGRY
                     LYISPSSRRLLGYEPEDLLGTDPYDLFHPEDLERIRSEAHEKALDGQGAVSVTYRVRK
                     VSGDYTWFETLTEPILDESGKVVRLQTALRDVSERKRAEEALARAARARAEFLAEVSH
                     ELRTPLTVIRGNAEVGLELDGHCEHEEILREIVRESSTMSRMVEDLLFLARSESAAPP
                     FRMVPVDAQALLAGLERRAGALAAERDATLDTTLLATGLVRVDPVRVEQAILALVDNA
                     VKYGPEGQCVILSSACTDGELRIEVEDRGPGIPETELPLIFERFYRLAEVGEPGSGLG
                     LSIAQTIAEAHGGRIEAESRLGEGTRMSFILPLLPNGGQARKRNM"
     gene            20804..21484
                     /locus_tag="VN178_14710"
     CDS             20804..21484
                     /locus_tag="VN178_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS82268.1"
                     /translation="MLILIVEDEAAIARFLKRGLNAQGHRVVCAYDGEEGTRLAVDEP
                     VDLVLLDISLPRLDGRQVLRRIRLRRPKLPVLMLTARDDTRNKVGALDAGADDYLTKP
                     FVFDELLARVRALIRRSDQAESVSIQTDGLTINLLSRHAWRGDERIELSSREFALLEY
                     FMRHGGQVLSRQQILSAIWDYDFEPASNVVDVYVRHLRNKVDPPDGPSLIQTVRGAGY
                     RFNAASNP"
     gene            21894..24005
                     /locus_tag="VN178_14715"
     CDS             21894..24005
                     /locus_tag="VN178_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HWS82269.1"
                     /translation="MRLIVAEKPSVGRDIASALGRHRREKGSLVGQDWTVTWALGHLV
                     ELAPPDAYGEEYKKWRLESLPILPGRFKVRVNHKTREQFNVVKGLMKSTSVTEVVNAC
                     DAGREGELIFAYLYGLSGCKKPVLRLWISSLTREAIRDGFHALREGAAMKPLEDAARS
                     RGEADWIVGMNATRAYSVRFSRPGNVLSVGRVQTPTLKLLVDREREIQDFKPEKFWTV
                     HARFARDGSTYDGVWFKKKENRLTEREAADRIAKKVRGGTGTVSKAQKKTATEKPPLL
                     YDLTELQRNANARYGFTAERTLRAAQSLYEERKLITYPRTSSRYLSGDLVGGLKKRIE
                     AAGALPDLAPFAEKLLGGEKLPISRRIVDDSKVTDHHAIVPTDKRSSGGLSPDETKIY
                     DLVARRFVAVFFPAARFENTTVVTEVRGETFLSRGRVVLEAGWRALYPGGVGGRKEKE
                     PPVLPPIEVGQEWDVAKVGVKEGETKPPPRYSESALLGAMETAGKLVEDEELRQQMKE
                     SGLGTPATRAATIERLIKVGYIEREKKILVPTEKGRSLIGLLGESPLSSPELTARWEE
                     RLAKMEKGAERRPDFMADINGFTTKLVQEVRGMEGERLAAPSRSREPLGVCPKCGAPV
                     IETKKAYGCSAWKKSGCDFAIWKQVSGKRLSVSQAKQLLTKGHTAKMKGFKSKAGKPY
                     AAALKLDGEHKVRLEFESSRK"
     gene            24067..25305
                     /locus_tag="VN178_14720"
     CDS             24067..25305
                     /locus_tag="VN178_14720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWS82270.1"
                     /translation="MKTIIVGAGLAGLTCAKVLRERGTEVAVFEASEGVGGRVRTDEQ
                     DGFLLDRGFQVFFTSYPVAGRHLDYGALEFRDFDPGAIVRRGAEESVLSDPLRDPRAL
                     VPTLLSDAATLGDKLRTLEMVARTSTVGISAGTEDGEADISTLEYLKMAGLSGRYIDS
                     FFRPFYGGITLNRGLTTSARVLRFTLRMLAEGSTVVPALGMGEIPRQLASHLRDGEIQ
                     LDSPVEALLREGGRVVGIRSVGKEHEADAVVVATDAPTAGALTGAAVPEGTVGEVCLY
                     YETDGLGTGKKILLNAEDGAFVNNAVEISNISEKYAPPDRHLLYAVALTGTNLPDEDL
                     YRKGIEDLSRWYPQADFRPLALRRIPYGQFAQPPGIHSRLPANTTQTPGLVLAGEYTE
                     DSSINGSMLSGEKAAGAVIA"
     gene            25302..26051
                     /locus_tag="VN178_14725"
     CDS             25302..26051
                     /locus_tag="VN178_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWS82271.1"
                     /translation="MSANGTPALKIEKLTKTYSNGFLALSGISLEVEAGKFFGLLGPN
                     GAGKTTLINGVVSLARPDSGSVEVFGKDAYREFREARRMIGVSPQEVNLDKFLTVEET
                     LLFHAGYYGVPRKKARQKAEELLEQFELTGKRGQRTNTLSGGMKRRVLFARALMHDPK
                     VLFLDEPTAGVDVELRYKLWGYIRELNRGGMTILLTTHYLEEAEALCEEIALINGGSI
                     AAQGSSEELKERYEAGNVEEVYMKVIGHDLH"
     gene            26038..26805
                     /locus_tag="VN178_14730"
     CDS             26038..26805
                     /locus_tag="VN178_14730"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS82272.1"
                     /translation="MIFTDRTFQTLLSREILRFTRVWMQTIVPQILTSLLYLVVFGIA
                     LGGRIKQIEGIPYLEYILPGIALMSLITNSQMNSSWSVFDAKRERYIDEVLISPMSDL
                     QIALAYSLGGMLRGVVMGAAVFVVGVPFVGVQIKHPLMLVMIGLLASFAFSSLGTMVG
                     ALATRVEHISLITSVIIQPLAFLGGVFYSVGMLPGILKVVTFFNPIFYTVDAARYVTL
                     GVSDLNPYPTLGMVCLIAAICFGGAWWAISRGPNLRY"
     gene            complement(26927..27268)
                     /locus_tag="VN178_14735"
     CDS             complement(26927..27268)
                     /locus_tag="VN178_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82273.1"
                     /translation="MASTDFAPLRVGVIVLTVGTALIHLYLGLQGFPLFILNGLGYLT
                     LLAALNLPIPRISQYRNLVCWVLVGYTALTIFLWILVGARNAIGYVDKVIEIALVALL
                     VLEASRRPWGR"
     gene            complement(27294..28514)
                     /locus_tag="VN178_14740"
     CDS             complement(27294..28514)
                     /locus_tag="VN178_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS82274.1"
                     /translation="MNSDVWRRRSPLVFLFVTVFVDMIGYGIVVPLLPFYAGPYASGA
                     VLVGLLGSLYAAMQFASGPFLGGLSDRHGRRPVLLSCLLGTSLAYLLLGLAQTLVYLV
                     FAVVLAGAASGTLATAQAYIADSTAREDRARGLGMIGAAFGLGLLAGPAIGGLLSLHS
                     LSAPALFASALALANCVFGYLTLPESHAPHMRKKVPLLRLDPISQLARILKMRNVRAL
                     LAAVLLLNLALAGLVNNFPLFSQARFGWGTTSNAFFFAFVGLCAVVTQGVLIGRLQPR
                     FGESRLLLGGLVLASLNLLLVALVPSGILLYPIVGLLALGMGLAIPSLTAIISNRTPA
                     ETQGRLMGGMQAILSLAMIFGPALAGLAFDHLGIPAPYVIGGALIILALIASAADLLP
                     GRRTRLVQRAKISG"
     gene            complement(28511..29410)
                     /locus_tag="VN178_14745"
     CDS             complement(28511..29410)
                     /locus_tag="VN178_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWS82275.1"
                     /translation="MDSISHLPTATEEFHASSGSDDLLERLTELCVEDLICAFGLGGT
                     SHGRALMESISRIPARRLARQILTYDRIVGESGLGTGGAWALKRLSRNTSIEGQKNVP
                     RGGPLLLVSNHPGLADAVALFAATPRDDLLVIAADRPFLEALPNTSRHLLTVAETSAG
                     RPGVVRAAARHLRGGGTVLTFPGGRIEPDPAILPGAVEALNRWSASGDLFARLVPGLV
                     VVPVVVSGVISPSALRNPLIHLRRRRRDREWLAATLQVLVPALRNVDARVEFGSPIHA
                     EAGSVGDAVIEETRRLMESRKPM"
     gene            complement(29556..29936)
                     /gene="folB"
                     /locus_tag="VN178_14750"
     CDS             complement(29556..29936)
                     /gene="folB"
                     /locus_tag="VN178_14750"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWS82276.1"
                     /translation="MSEDRILLTDMVFHGRHGTLQAEHELGQPFIVSIELRLDLRGAG
                     TSDDLTKTVDYSEVHRAARDIVEGLPVHLTETLAERIAAATLDEHPAVEAVKVKVAKP
                     HVRLDDTVLAGSAVEILRRREPGT"
     gene            30143..30868
                     /locus_tag="VN178_14755"
     CDS             30143..30868
                     /locus_tag="VN178_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HWS82277.1"
                     /translation="MIGEEQYPSAGKGASIRPSVTVSYAQTLDGRVATRTGESKWISC
                     PDSLRFAHEMRASHDAVLVGAGTVCKDDPRLTVRHVPGTSPLRVVVDSTLRTPPDAAV
                     LADGAAPGTVFAVTDRAPEESLKKIHSLGATVIKLPPDPGGRVELAVLLEELAASGVE
                     SVMVEGGATLITGFFRARLVDRIAVCVAPKILGVGIEAIGDLGIRDLSRSLTLADTAV
                     EPYGVDLILRGRIEYPGGAPDRG"
     gene            30846..31898
                     /locus_tag="VN178_14760"
     CDS             30846..31898
                     /locus_tag="VN178_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HWS82278.1"
                     /translation="MVPRIGVEIEARALWFTAPRTAALRAETVRPPGPQEVRVETIAS
                     AVSAGTEMLVFRDEVPHELPLDLPTLAGSYAFPIKYGYAAAGRVLDTGSGVEYLSPGD
                     PVFVHHPHQEVFVVPAQMPVRLPDELDPVLGVFSANLETALNIVHDTPVRLGETALVF
                     GQGVVGFLVALLLKLSGAGSVLVVDPLEERRKLALAAGADVAFAPGGLNDRVMETTGG
                     RGADVAVETSGAGAALQSAIDAVATEGTVVVASWYGTKPVTLELGGHFHRGRVRLRSS
                     QVGRINAELAPRWDRARRMETVLGLLGRLELKELISHRIPFEEAPEAYTLLEERPQEA
                     LQVIFTYEGVRGGQDV"
     gene            31891..32268
                     /locus_tag="VN178_14765"
     CDS             31891..32268
                     /locus_tag="VN178_14765"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWS82279.1"
                     /translation="MYEIYVATRFEAAHRLIGDFGPATRTHGHTYRMEVILRGQRLGD
                     DGTLYDIGELGQAVGELAGSMHYRDLNEVPGLQEVNTTAEAVASYCWEKLAEPLRGQG
                     LDSLTVRIWESSDAYAAREDTLD"
     gene            32279..33349
                     /locus_tag="VN178_14770"
     CDS             32279..33349
                     /locus_tag="VN178_14770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWS82280.1"
                     /translation="MRVAFITVGDTGRKTGGYLYNGRVISGLRKRGFEIEEFVAGRAS
                     PDEQRKAAPRFGTTFDPSGYDAIVVDALARIAVAPHLDLWLSRRPVVALVHELPSLAG
                     GGSGPETIACERDYEEPLLRAHRLVAVSNNGRNVLLGRGVSSGRIRVVPPGFDGIPEG
                     VGPHVQGSGPVRALCVAQWIERKGILTLVEAWALRERKGAVLELIGETDADPDYAVLV
                     GDAIEAAPRGSIIVSGCVDNAALGASYTGADFFILPSRYEGYGIVYAEALACGLPIIA
                     CDIGPVPDLVGREAAVLIRPDDKEELSAALDLLIGDSALRASMSAAAIRRARRLPRWE
                     DTVSGFEQVLRTAAETGKQGPM"
     gene            33359..34222
                     /locus_tag="VN178_14775"
     CDS             33359..34222
                     /locus_tag="VN178_14775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS82281.1"
                     /translation="MDRRDLREQNRVSWNAVVRAHDSHRGDLSRFLRGGGSTLFREEH
                     DLLGELEGKSLVHLQCNSGGDSISLARLGATVTGVDLSDEAVSSARDLADKTGIRVAF
                     ERADVYDWLQKAAREGRVFDVAFASYGVICWLPDLRSWAQGIAGVLNPGGRFVLVDFH
                     PAADVFDKDWNHVHDYPSGGVPLLVEEGVDDYVAASGGGLTPAGFVEGVRDFENPEGC
                     YLFRWRLGEVITALAEAGLSITALEEYPYANGERKFAGMRELPGKRMVPPDHIPAIPL
                     MYGIRAEKAES"
     gene            complement(34244..35278)
                     /gene="cax"
                     /locus_tag="VN178_14780"
     CDS             complement(34244..35278)
                     /gene="cax"
                     /locus_tag="VN178_14780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HWS82282.1"
                     /translation="MLAKVAYGVTAALTVAVVVAELLHAPTLLIFAATALALIGLAWV
                     LGQATESLGHHSGPRIGGILNATFGNAAELIITIFALSAGLTTVVKASIIGSIIGNVL
                     LVLGASLLFGGLKNGIQHFSAEIAGINASMLAIVAAALSLPTIFAATSPDPSPTSLSI
                     EVAVVMFVLYVLYLVYILRSPGGAAQGEGHAPFGRTASLALLFVATAAVAYVSEAFVG
                     AIEPLTEEYGISELFVGVILVPIVGNIAEHVVGVQIAYKNDMDFSMGISLGSSIQVAL
                     LVTPILVFLGPLLGHPLRLIFTPLELGALGAAVVVTGFAALDGRSNWLEGAMLCGVYI
                     IAALAFFFSP"
     gene            35370..>35624
                     /locus_tag="VN178_14785"
     CDS             35370..>35624
                     /locus_tag="VN178_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWS82283.1"
                     /translation="MIDYHLHVIAHGDRPMTVENILSYCEVAKERGVSQMGITEHDRY
                     LEEIDLAAFQEARELSQDVDLRLGIEIDYVPGAEERMENDA"
BASE COUNT         6584 a        10876 c        11665 g         6499 t
ORIGIN      
        1 catgctccgt tttcacgaag gaaaggagaa ggctgccccc gatctcgatg caaagaaggc
       61 gtggcggccc gtcacggctg ggttgagggt gtgggaaacg gagatgtcct ccgaggaagt
      121 cgagagcttc gaggcggccg caggagacct cctcgacgag ctaggctacc cgcgggccgc
      181 gccccgtcct tccccgcggt tcgtcgagga ggtggctggg gtccggcgcg ctttcaccga
      241 agacctccgg gtccagggcc accggattcc ggagcgctgg tgaaccctta cgtgttcatc
      301 gtcggggcgg cgcgatcggg gacgacgctg ctgcagcgga tggtagacgc ccaccccgac
      361 atagccgtta tccacgagtc gcactggatc ccacgcttct acgaagagcg gatcgggctg
      421 accgccgaca gcacggttac gctggaactc atcccgaggc tgctcgacga ccgcaggttt
      481 gtcaagttag gcatcagcca gagagacttg gagggtctaa tcccctcgga ccggacgatc
      541 tcctacgcgg actacgtcgc ccgtatcttc gacctgtacg ggcgggccag gggcaagcgc
      601 ctcgtcggcg aaaagactcc gggctacgta cggagcatcc ccacgctgca ctatctctgg
      661 cccgaagcga agttcgtcca cctcatccgc gacgggcgcg acgtattcct gtccatgaag
      721 agctggaaga gggtggatcg ctcggtcggg cgcttcgccg cctggaggcg cgatccggtc
      781 ccgagctcgg ctctctggtg gcaatgggat gtcgagcggg gccgtgaagc ggcgaacttg
      841 ctgggaccgg agctttacca cgagcttcgc tacgagaggc tggtctccaa gttcgccgaa
      901 gagctggcga agctatgcgc cttcctcggc cttccatacg acgaggccat gctcgggttt
      961 cacgagggac ggacgaagac caagcccggg ctagacgcga agagggcgtg gctgcccctc
     1021 accccgggtc tccgtgattg gcgaacggag atgcccgccg cggacgtgga aatcttcgag
     1081 gcggcggtcg gcggcctgct cgacgatctc ggctacgagc gcgccttccc caacccctcg
     1141 tccgaggcca tagaatgcgc ttcggagacg cgccgccggt tcgtccgaga gctggacgaa
     1201 cgagaacgga gaacgccggt gaaccggcga agttgaaccc gtgcacagca tcctcaccct
     1261 tcgaccgttt tcgtctgatg ccagggcaga aggtcgccat tacttgcttt atcgcttgat
     1321 tgtatgatta gcttttcagg atgggcgtac ctgcggggat gggttgagga agatagcgtt
     1381 tatcaatgcg aagggcgggg tcgcgaagac cacgtgctgc gtaaacatag gcgccgggct
     1441 cgccttgggg ggctaccgta ccctcctcat cgacctcgac tctcaagggc aggttaccaa
     1501 gagcctgggc gtgcaggctg gtgagggatt ggcggaactc ttgcagggcg atgccggcct
     1561 gaaagacgta cagatagagg cccgcgagaa cctgttcgtg atcgccggcg ggggagcgct
     1621 ctccggcgcg aagcgactga tctcccggat ggacatgcgc tcggagttgg ttttgagcga
     1681 ggccctcgag gacttgaggg gatacgactt cgtgctcctc gacacggcgc cctcgtggga
     1741 cgtcctcaac gtcaactgtc ttttctacgt cgacgaacta ctgatcccgg tctccatgga
     1801 ggccctggcg ttgcagggca taggcgactt cctcggccgc gtggatcagg tccggcgcta
     1861 cagggagggc ttgcgggtac tcagcatcat ccccaccttc tgcgacggga gggtcagaaa
     1921 gagcgccgag atactcgagc aactcgagcg tcattttccc gacgccctgt ggccttgcgt
     1981 gcgctacaac tcgaagcttt cggaagcgcc gggcttcggc cagcacatct tcgagtactc
     2041 gaaaaggagc aacggcgccg ccgactacgc ccttctggcc gaacggctcg ctaaggggag
     2101 gtgaaaggga acatgggcaa gtcaaggaaa gtgccaaagg acctcatggg aaccatcctt
     2161 agggagagcg ccgggcctcc cggaggggaa cgcaattctc cggagtccgt cgagcctggc
     2221 atggtctcgc ccgcttccgg cggcgaaggg acaatctcct gggaggatca accctggctg
     2281 gtgggtacca cgttcaacct ctccaaacag gtcggcagag agctggaccg gctcaggcat
     2341 gaccttctgc gtcaggaaga catccacacc tccaactccg agatcgccga ggtggcactc
     2401 aggatcgcca tcgaaggcgc gcgggagcgg gggagaggga gcgagctggt cgggaggctt
     2461 ctaagcggac ttggcggccg ggctacgtac ggggccggcg gggcaggttt ggcccggcgc
     2521 agcgtcgacg ggtcgggacg catcctcgag accacctaca acgaagaggg acaggtgatg
     2581 gatgaggagg aggtcggcga cgtcaccgac ctgcctgtgg agacggaata cgtggacgcg
     2641 cagggtaggc ttttgagcct ggcgagggac gagctgggga ataccttcga gtgggttacg
     2701 gacgaggagc tcggcacgct ggaggcgagg atcttccggc cctccggctg agaagatcgt
     2761 gtcctcacgt tccctgaacg ccgctggagt cggacaggcc aaaatccact ggtaaaatca
     2821 ccctgggcat gagcatccca cgttattaca ccgtcggggc cgtcgcgcag gacctgcggg
     2881 cgctcaaagc cctggacgaa cgcctagaag accttggtgt cccagccgac tcgctcctcg
     2941 tgctctcgcg gcgcaaggac gagcgactcg tgggcgtaac gctccccggg gccaggaccc
     3001 gccgggtcga gtcggggccc acacgaacgc agtggtttga gttggggagc acgtacctcg
     3061 gggtgacggc cgtgagcgtc ctaatgggcg ccgtccatcc gccgaccggg atcgccgtac
     3121 aggccgtcat taccctggca acgataatcg gtttgatcct ctaccaccgc aggccgcacc
     3181 tgcagaagaa gctcctaacc atgggcctcc ccgagagttt cgccggggag tgggaagcgg
     3241 ccttccatga cggcttcgcc ctggcgctgg ccacggttcc ctctgatctc cacgacgaaa
     3301 cgcaggacgc ctttttggag gactccgaac tcggatctcc gctcgccgta gacaggcggc
     3361 cggtgttgta gagctggtca gtacggcgct tcgcgcctct cagctttcgg ccatcagctt
     3421 tcggcttccc cactcgagac tcgtccttct gcacacgagg aaccattgct tgaggcggtc
     3481 gggttctacg ctcatgacga cggtccggga cgactagctg agggctgaga gccgccgtca
     3541 ggcggcgggc tgagagcgaa ggcgaaagcc tgagcgtgct gaaagctgcc aagctgctaa
     3601 gcttggccag tgatcattcg cgagcggcca gactcttttc tgctcttcgg gcagcacgag
     3661 cacgccctgg tagcgggcga gttcgcgcgt cggtggaggg agaggccgaa ccccttcgag
     3721 tccacggtct acgccgtcgc caaccacgac ctggcctggc gcgaagctga caggggggtg
     3781 ctgtggaacg aggagaagga tagaccttac tcctttgtgg actatcccct ggacctgaaa
     3841 ctgccggccc agagacgggg gatagatctg gtggaggaac atgatcctta cgcgggatgc
     3901 ctctgcagca tgcactacgc ccgtttcctg ctcgactccg tgcggcccga ggaggtcgag
     3961 ttccgcgagg acgaggtggg acggcaggac aggctcgagg agaggatggc cgaaaatgag
     4021 ctggagaatc tcgagcgaaa cttccgctta ttgcggctct gtgacggcct ctcgctgtac
     4081 ttgtgcctca acgaacccgg cgacggagac ggcccgccgc cctatcccgg agggttcgag
     4141 ttcgaaggga cgcgcttcgt gccggtatgg gaggatggac agacgttgcg cctggatccc
     4201 aaccctttct cggaggcatt cgggatcgag atcccctaca ggatcatcgg cacggaccgg
     4261 cggcagctcg gcagcgacat actggaactt cgggtaacct gttaggagcg ccggaaggcg
     4321 cttccttaga gattcgcttc taccatagaa aggacccatt ttgagagacg cgtcgtacga
     4381 tttcttgaag aggctgcttt cgacgccggg gccaagcggt tacgaagagg ccgccgcgga
     4441 ggtctggagg gaggaggccg ggaagttcgc cgaggtcaag ggcgacagga tgggcaactc
     4501 cttcgccacc atcggggcgg gcggctcgcc gaaggtcatg cttgccgggc atatagacga
     4561 gatcggggtg atgatcaccc acacagagga gaatggcttg ctccgcttta ccggcgtcgg
     4621 cggctgggat tcgcaggtgc tcgtgggaca gcgcatccgg ttgctcacga aggacggcga
     4681 gttggtcggc gtgatcggca agaaggccat ccacgttatg gagcccgatg agcgcaagaa
     4741 ggtctccgag ataaagagcc tctggatcga tgtaggagcc aaagacggtg aagaggccaa
     4801 aggcatggtc cgcgtcgggg acgttggcgt aatcgatcag gacctcatag agcttcccaa
     4861 cgggcgtctg gcctcccgct ctgtggataa ccgcatgggc gcgttcgtcg tgctcgaagc
     4921 cttgagattg ctctccgaag aggaagatct cggcgccgag gtcgtggccg tggcctgcgt
     4981 acaggaggag ataggtctat acggagcccg cggcgctgcc ttcgggctcg accccgacgc
     5041 ggccatcgcc gtcgacgtga cccatgctac ggacaccccc ggcgtctcca agaacgagca
     5101 cggggaccac tccctgggcg gcggccccgt tataaagcgg gcgtcgaaca tctcgcccat
     5161 cgtctccgaa ggcctcatcg cggccgcgga gggcgagaac atatcctatt cgctggaggc
     5221 cgactcgcga tcgacgggga ccgacgcgga cgccatacag ttcgcgaggg ccgggatcgc
     5281 gacggggctc gtctcgtgcc cgaaccgcta catgcactct cccaacgagg tcgtcgacgt
     5341 agaagacgtc gagaactgcg cccgcgtgat ctccgcctac ataatgacgc tcagcgcgga
     5401 gactaacttc gtccgttgat ccaccagtcc ttttgactac tgaggaggaa ccaaagcggc
     5461 aggcctcgat aggagtcgag gccgcgctgc tgttgacggt cttcttcttc gggacgaact
     5521 tcacggcggt aaagatcgtc gtcgagagcg tcccgccgat actcttcgcc gcaacgcgct
     5581 tcaccctggc cggactcctc ctgctcctct tcgtgccctt cctcgaaccg ggcagtcgcc
     5641 tgcgccgcgg ggacttcctg gccgtgctcg gcctcggggt ggtcggcgtc acactcaccc
     5701 agacggtctt caccatcggg gtgagcctga ccacggcggc aaacacggcc ctcgtctact
     5761 cgacggcccc cgtttggggt atgctcctcg gattcgcgct cgggctggag cggccgaggc
     5821 ccgccggtat cctcggcatc gggctctccc tcctcggggt cgggttcatc gtgtacggcg
     5881 ggctcgagtt cgccggcacc agcctcgccg gtgacgccct catactggct gcggccgtgt
     5941 tctggggctc ctacaccgtc ctctcgctct cactcctgaa gcgctactcc cccgttgccc
     6001 tcgccgccta cgccatgacc ctcggcggcc tcgccgcctt cccgctcgca gcactcgacc
     6061 ccggcggtct cgatatcgga tcgctcgacg gcctgacgtg gggagcggcg gcttactccg
     6121 cgctctgctc ctccgccttc ggcttcgccg cctgggggta tggggtctcc cgcgtcgggg
     6181 cgaaccgggt cctgatctac cagtacctag tcaccctgac tggtgttact acgggcatcg
     6241 tactcctcgg agaggacttc gggctcgaac aactcgtcgg cgcagtcgtt atcatctgcg
     6301 gcgtctacct ggcgagaaag agatagtcga gggtcgcatg atcatagttt gcatcacctg
     6361 tgagtccgga tgcaacgcag ggagcgcgtc cctcaaccgg gacgcgctcc gcgccggccg
     6421 aagttcggcc gggggagggt gagaggtgac ttactcgcgt ctctcggcga gatctacctg
     6481 gagcgtgatc ttctcgccgt tcctgagtat ttctagtcct accgtatcgc ccggcatgta
     6541 gttgcgcagc gccgagagca ggtcgcccga gctacggacc tcctcagaac ctgcggcggt
     6601 tatgatatcg ccgcgccgga ttccggcgtc gtcggccggg ctaccgggct cgacctcttc
     6661 gatcagggcc cccgagtcta cggggcttcc gaacctgctc gcggtctctt tggtgaggtc
     6721 ggagaggtag acgccgacgt aggggtggac ggcctcgccg ttctcgacaa gctggtcagc
     6781 cacggagatg gccgtctcgg aaggaatggc gaagccgatg ttctcggccc cggtctgggc
     6841 cggcggcagg taggccacgt ttatgccgat tacccggcct tcacgatctg ccagggcgcc
     6901 gcccgagttg ccaggggaga tggcagcatc ggtctggatg aggtcgacca gggagctggc
     6961 ctgcgtacca cccgtgtact cggcggggac ctcacgcccc gtgccgctga tgatgcccga
     7021 tgtgaccgta gactcgaagc ccgaggggct gccgacggcg accgccatct ggccgactat
     7081 cgggtcggcg cccgcgaagt ccgccgcggg caggtcgttg cggcctaccc tgacgacggc
     7141 gatgtccgtc gtcgggtcct cgcctacgac ctctccgcgc tccgtggtgc cgtccgcgaa
     7201 ggcgacctcg acgtcgcgcg agccctctac gacgtggttg ttggtgatga tgtaaccgtc
     7261 ggagcggtag atcacaccgc tccccagacc ctcttgcttc tgcgcaccgt acggcgtctg
     7321 tttgattccc gagacgttga tctgcaccac ggatggactc agtacggaag cgaccctggc
     7381 cacgggttcg tcggcaggaa tgcttgcacg ggccgactcg accggatctg cggtctgcgt
     7441 tctgcccgta tccgggtcga ggtagatgac gccgaggacg aaagccagca ccgtcagcgc
     7501 cacgagcggc aacaacaaag tgcgccgtaa ggtgtgtact ttcataccgt tacccccgtt
     7561 ttctgtaggt tctcggataa gttggcaacg cgcctcctcg ctgtgtgatc tctgggacat
     7621 agtctggagt accaacatgt gaagagtctt tgcggtccat gagcgttttc acaggaaagg
     7681 tcccaagggg ggcagatctg ctccagagcg gcgataatac aagggtgatg cggatactgg
     7741 tagtagagga cgaagagagg ctggcgcgtt tgatctcgcg ggtcctcctc gaggagggct
     7801 acgccgtcga gacggaggcc aacggccggc aggcgctcat gcgggccttg gcggatgatt
     7861 acgatctcct gatcgtggac tggatgctcc ctgatctgag cggcgtccag ctggtcaaga
     7921 ggctaagggc ggcggaggtc gggacgccgg cgatcatgct gaccgcccgc gaccagatcg
     7981 aagaccgcgt ggaaggcctc gacgccggag ccgacgacta cctccccaag ccatttgcct
     8041 tccccgaact gctcgcccgc gttcgggctc tcgcccgccg cccgtgggct gaggagagag
     8101 acggagcgat cctcggggcc ggcgacgtga cgctcgaccc cgtgcgtcac gtggtccgcc
     8161 acaggggaga gaccgtggac cttacggcca aggagttcgc actgctcgcc accctgcttc
     8221 agcgccccgg gcaggtcttc acccgcccgg tcttgctcga tacagtgtgg ggcgcctcgc
     8281 ccgaggtcta cgccaacgtc gtcgacctct acatctccta cctgcgcaag aaactcgacg
     8341 gtgacggcga gacctcccac atccgaaccg tccgtggtgt cggctacacc ttcgagccgc
     8401 aaccgggacc gcgctgatgt tcgatcacat ccggcggcgc ctgacggtgg gctacgtggg
     8461 catactggcc ctcaccctgt tggtcttcag cgtcgtagtc gtgttcatgt tctacctgcg
     8521 ggcgagcgac caacaagacg acttgctcct gcagaaggcg gaagacaagg ccaacagcgt
     8581 gttgagcggc aaagacaggt acagcgtggt gaaagcgact accgattacg acgtggccgt
     8641 gatcgtactc ctgcccgatg gttcgaccta cggagatctg gacgaaacgt ccttctctct
     8701 cggcctccct tacgcgtatc tggcccagag cgccgggcag gagaggcgga cgatcaaagg
     8761 gacggttccc gggcctgggg gcgccgtgag ggtgatgagc atccccgtaa agaggtacgg
     8821 agagttcggg agcgtggtcg cggtggtaca ggccgcccag tcgcgtcagg cggtgacgga
     8881 gacggtggaa cggctgatct tcgtgctggt cctctcgggc ttgggagcgc tggtactggc
     8941 tgcggccggc gggtttttca tgtcgcggcg ggcgatgcgt ccggtacagg aagcgttcgg
     9001 caggcagagg acgtttatcg ctgatgcctc ccacgagttg aagacgccgc tgaccctgat
     9061 ccgggccgac gccgaggtcc tctcccgcgg catcgacgac cccgacaagg ccactgacaa
     9121 ccgtgagctc gtcgacgact tgctcggcga gacagacagg atgagcgccg tgctctccga
     9181 cctcctgcta ctcgcgcgtc tggacgcgca gaaggtctcc gtctcccgcg aacctttcga
     9241 cctcgccctc gtcatctccg agacctcgga gcgattcgcc gtccgcgcca ggaccgaagg
     9301 gaagaacctc gaggttcacc actcgggcaa acttcctgcg cgcggcgacg cgacccgtac
     9361 gggccagagc ctcgccgccc tcctcgataa cgccctgcgc ttcacacccc cgggcgggac
     9421 cataacggtg gcgagccgca tcacggacaa acacgtcgag gcgatcgtta cggataccgg
     9481 tccaggtata gcccccgaga gcttggaccg aatcttcgag cgtttctaca gggacgacgc
     9541 gcacagcgcg gccaggagca ggggaccttc cggcggtgga acgggtctcg gtctcgccat
     9601 cgcccgcgac ctcgcccgcg cccagggtgg cgagatcagc gccgaaaatg ccgaaggagg
     9661 cggggcaaaa ttcacgctca gcctgcccac tggttactga acctcacccc tgccaggtcg
     9721 ctgcgctcga ataacgagct cccctgaccg cgatcaggga ggtggagttt gagcagcgtg
     9781 tcgttgtggc cgtcgaagac gaggggaagt tcggctccaa ctagaagccg ggttcccaga
     9841 tgggctcgag ggcgaggacg aagccaggga ggacggggtc gccgggtttg tggacgtgga
     9901 ctttgcgctc ctccgggaga gctttactcc accattatac ttttccctcg gatcaccccg
     9961 aaccacaaaa agcggggccg cgtgaagcga ccccgccgat gatgtgccgg gtgtcccgat
    10021 caatacttcg ggacgctcgg gtcgatctcc tcgctccagg cggtgatgcc acccttgacg
    10081 ttgtagacgt tttggaagcc agcgtcttgc aagagcttga cggccttcgc gctgcggccg
    10141 ccggtcttgc agtgaacaac gaagtactcg tcctgatcca actccaccag gcgctgcggc
    10201 aactcaccca agggtatgag cttcacgccg atgttggcga cctcgtactc atgcggctcg
    10261 cgcacgtcga gtacgttgat gtcctcgcca tgatccatct tctgcttgag ctctccgacc
    10321 gtgatctcgg gcacgccgtt ctcccgctcg gtggtctcgg cctgcgggat gccgcagaac
    10381 tcctggtagt cgataagttc tgtgacggtc gggttctcgc cgcagacggg gcagtccgga
    10441 tccttgcgga gtttcatctc ccgaaagcgc atgcctaaag cgtcgtagag gagcagccgt
    10501 ccgatgagcg gctcgccgat cccgagtata agcttgaccg tctcggtggc ctggatggta
    10561 cctatggccc caggcaggac gccaagcact ccgccctcgg cgcagctagg gacgaggccg
    10621 ggagggggtg gctcggggta gaggcagcgg tagcaaggac cttcctcggc gtagaacacg
    10681 ctcgcctggc cctcgaaacg gaagatcgaa ccgtagacgt tgggcttgcc cagcaggacg
    10741 cacgcgtcgt tcaccagata acgggtcggg aagttgtccg tgccgtcgac gatcacgtcg
    10801 tagtcctcga agatctctag agcattctcg ctggttagcg cctcctcgtg caccctgacc
    10861 tcgacattgg ggtttatgtc ctcgagcttc tcgtaggcgc tctggacctt gggacgcccc
    10921 acatccgagg tgccgtggat gatctggcgc tgcagattgg actcgtcgac cacatcgaaa
    10981 tccacgatcc cgatggtccc cacccccgcc gcggccaggt aaagggcgag cgggcttccg
    11041 agtccgccag tgcccaccgt gagtaccttc gcggctttta gctgcttctg cccctcgagg
    11101 gcgacctccg gcatgatcag gtgcctgctg tagcgtgcga tctcttggtt gcttagctcg
    11161 tgtgccccgt tgggcgattc gactagtggc tgtcttgtct gcatttgaag ataaacctct
    11221 tgctcgaaaa agtgagaaaa acgaccttct gaaaactacc tacatcgccc ttcggcagag
    11281 cccccggcga tggatgggac tatggagatc tcgtcgccat cggcgacgcc ggtgtcctgg
    11341 ccttccagat aacggatgtc ctcgtcgccc ttgtagacgt tgacgaacga gcgcagcttg
    11401 ccgtcacccg tatacagatg ctgcctcagg ccgggatgct ccgaggcgag gttaccgagg
    11461 gcctcgcccg ccgtggcgcc gccgacctcg acctcggcgt taccgccggt gtactgtctt
    11521 aagggggttg ggatcttgac cttgggcata tctgagtctc cttatcctac gattgtctct
    11581 tcgtcgtaac ccgagcggtc gtctctgagc gtccacgagc gcatgtcttc tacgttctcg
    11641 gcgcccagcg aggctatcac gtaggagtag ccaggccagg cgtgctcgcg gtcgtactcg
    11701 gaaggctcgg cggggtggtc ggggtgtgaa tgatagaagc cgagcagccc catctcctgc
    11761 tcctcggccc gctcctcgaa cttctttacc tccagcggtt cgatgagaaa gcggcggctg
    11821 cgctcctcct cgtgcgtgtt ctcggcccgc cacacgtcga cgacgacctt gatgccgccg
    11881 ctgtcgatgc cgaccagcgc gccggcgcac tcctcggggt acgcctccct ggcgtggtcg
    11941 tggatccgcc ccaaatcccc cgcgcctatc ttgagcgcca ctcactcctc ccagaagttc
    12001 tcggagaggt acttgtcggc cccgtcgcaa aggatggtga cgaccgtccc cgactctatc
    12061 tccctcgcga ccccgagcgc cgtcgcgacc gccgcgccag ccgagaggcc gatgaggatt
    12121 ccttcctcgc gcgccacccg cttgaccatc tcgtaggcgt cttcggtcct cgccgcccgg
    12181 ttctgatcgg cgatggtcgg gtcgtagatg gcgggcacga tagcgctctc catgtgcttc
    12241 atgccctcca ggccgtggaa tgccgagtcg ggttcgaagg agataacctc tatcgccggg
    12301 ttgtactcct tgagacgggt cgccgtaccg acgaaggtgc cgctcgtgcc aagcccggcg
    12361 acgaagtgcg tgatctcgcc gttcgtctgc tcccagatct cgggcgccgt cgattcgtag
    12421 tgcgagcgcg ggttggcggg gttggagtac tggtccggat agaagtagcg ttcggggtcc
    12481 tcggcgtaga gcctccgcgc ctcacggatg gccccgtccg agccctcgtc agggtcggtc
    12541 agaacgaagt ccacgccgta ggcgcgcagg atcttcttcc tctcctcgct ggcattcttc
    12601 ggcatgcaga gccttaccct gtagccgagc gcggcgccga tccaggcgta cgctatcccc
    12661 gtgtttccgc tcgtcgcgtc gaggatggtc ttctcgggcg tcagagagcc cgtcttctcg
    12721 ccgtccctga tcatccacaa ggcaggacga tccttgaccg atccgcctgg cgtataccac
    12781 tcggccttgc caaggatgtc gacaccaggg atctcggccg agatgccagg taactccagg
    12841 agcggggtgc cgccgacaag ctccgtaacg ttcgtccccg cccgctcatt aatccgagta
    12901 atcatgtcgg gattgattat acagggtgta ggatcagcgt ccagagaagc gagatgagat
    12961 acccgacgtt atagccgaga ggcaaatggg aacctcccgg ctatggttgg tgcgttgcgg
    13021 aacattgtac ctgaatctag tgcagctatg gtacggctag aatgccgatt atcccaacgt
    13081 gtccagaatt tccggcaggg tacggcggcg gcaggtgaag atcggggtat ccagctcggt
    13141 gtaggagctt gcttcgctct ccctgagttc catcatcagg tcctggaact tctctggaga
    13201 ttcggcctcg aaggagagga tgaactcgta gtcgtctatc ccgaagcagt aacccgtgtt
    13261 gttctttacg ggatagtgtt tgcgtccgat ctgcatatgc tcgttcatca tgcgctggcg
    13321 ctcttccatg ggcaaggcgt accacgaccg tgtcttgacg aagggataga cgaagaggta
    13381 ttcgccttct cccgggacga tgtacgtgcg attctcgaag cctggcgtat gctcgccgac
    13441 gtagatggag cgcctgctga gggcgaagta cgagtaggtg atgcgcaggt acttcccgag
    13501 gcccgtcttc aggagggcgc cggtcatgct ctcgaaggcg tcgaggtcgt aaccgatcct
    13561 ccacaacatg aagtccgcgt ccgcgcgtag acccatgagc gagaaagtgc gcaagagcat
    13621 ctctccggaa tgcatctgtg cggcttccag gaactcccgt ttgccccgcg cgcgctcctc
    13681 gtcagggagg cgtcgccaga agggatccaa cccgaagaag acgtagttta tgtactgagc
    13741 aggcagtttc tctacttgcg actgctgttg gcgtgtcctt gtctcggtca tagtgttact
    13801 cccttcggtc gtaacggctg tcagccatca gctatcggcc gtcagcttct tgtccttgta
    13861 gctgattgct gagtgctgac agctgactac ttctctaacc cgaagtcgtt ctcgtacaat
    13921 tcggcgacgc gggagagctc ttctgacgtc aggtccggcg tctccgaggt cgccgcgaac
    13981 tcttctatct gctctttgcc gtagatgttg gggagggtag aggccacttc gggggaggcg
    14041 aggacgaatt tgagcgcggc ctggccgagg gtgcgcccgc cggactcggt gaggaaggag
    14101 agttgctcca ccttcttcag gcccgacgtg agccactctt tcggacggtg acgacggtgg
    14161 tcgtttttgg cgaaggtcgt gttctcgtcg tagtggcctt cgagcatccc cgaggagtgg
    14221 gggacgcgta cgatcaggga cgtgtccgtc tctctggcgg cctcgatcag gccccggccg
    14281 gggtcctgtt cgaggaggtt gtagatcatc tgcacgcctt ctatttcgcg ctcgcgcatc
    14341 gcccttacgc cttcttcgat ccagcctatc ttgggcccga gggcgatgcc gtaggccctg
    14401 atcttgccct cgttccgaaa ctcgtccatg agcgcgaaca gcccgtcgtg ctcgatggcg
    14461 tccatcttgg tgttgtggag ctggaggagg tctatgtagt ccgtgtcgag cctcttcagg
    14521 ctctgctcca gggcgaagcg gataaaccct tcggaccagt cctgggggcg ctcctgctgg
    14581 ccgcgtcggg ccgtatgatt gtagaagtcg taaccgatct tggtcgagat caccacctcg
    14641 tcgcgcacgt gcccgaaggc gtcagcgagg agcgtctctc ctttgcccga gccgtaggtg
    14701 tccgcggtgt cgaagtagtt gatgccccgc tcgcgggcct ctctgagaag cctcacggac
    14761 ctctcgtcgt ccacctctcc ccaccagccg gtcgagaccg tccacactcc gaagccgacc
    14821 tcggagaccg ctatatcggt gccgcccagt gtacggtatt tcatcttccg ctccctgttc
    14881 gcgttcggat cctggatcag atctactaca ttctagcccc ggacgctgtt gactttctct
    14941 caatccgcac tcgagtctat aatcgcacga tgctggagat gcctctgtat cccgcgccgt
    15001 gatcgcccgc tacgcgggcg gggagagcgg ggcgcggagt gcggcccgct ccggttctgt
    15061 ggtggtcgtg gtcgacgcct tcagggcgag cacgaccatc gcggtcctcg tctccaaggg
    15121 tgcaagggtc gtgcccgtca cttcggtgga agaggcggcg tccgcagaga cggacttccg
    15181 catcggggag cgggggagcg cgaaggtggc cggcttcgac ttcggcaatt ctcctacgga
    15241 gatcctgggt tcgaagatgc ctccaggctc ctcggtcgcc ctctcgacca cgaacgggac
    15301 acgtgtcttg gaggccgccc gcggcgcgag caccatcgtg accggcgcct tcgtcaacgc
    15361 cggcatgctg gccgaggagc tggccggagt ggcccacgac ggcgcagagg tagtggtcgt
    15421 cgggtgcggc tgggagggtc gccgcgcttc ggaggacgag gctgcggcgg gcgctatcct
    15481 ctaccggctg tactctcgtg gcgtcgaact ggacgggcgg gccaggagcg tcgtcgaggg
    15541 ttatctgtct cgccccgtcg gggcgctgcg gaacaacagc gcggcgcgga ggctcaagcg
    15601 tctcgggtac gagggggacc tcgagttttg cctcgccgag gatacggtgc ccgtggttcc
    15661 gcgcctcgtc aacgatgctt tcgccgggtg ggctctaggg tcccggtggc ctcgccgatc
    15721 ttcggcccga gggccaacac aaaaagccga gatttcttct tgacatgggg atggcgcgcc
    15781 attaccctgg agtcagcgaa ggcggatcaa atgttacaaa ctccgtcgaa tccgcgacgg
    15841 gaaggaggtg agtttacaag tgactatgat catcacccac gagaagaccc gcgaggtcac
    15901 ccgcgaggac gtggaccggg ccatagacgt catcgagcgt cacctcgaga aggaggccag
    15961 gacccgcaac ctgctggacg agcaggcgat catgcgcccc aaggacctgt gcaagcagca
    16021 gggcctggat atggttgccg agttgcgcgg catgatgtag gttcgcgaca acagcaaggg
    16081 cccatgaagg gggatgcaca gtaataatcc ctgacgctct catggaggcg ttcgagggct
    16141 gtccatcccc cttcacgagg ccggttgagg gccggagaga tccggccctc ttagcatgtc
    16201 agcacgctcc ggcttcgcct ccgcttgtca gcctgccgcc tcgctgtact cggcggctcg
    16261 tcagcatttc agctaatcgt cccgagacta gctacgggcg tggaacccaa ccgccgccga
    16321 cagaggttcc tcatacgcag cagggcaagg aaggaacggg agaagctgac aagatgacaa
    16381 gcgagggcct tataggcccg agcgtgctca ctagctgaag tgctagaaag cggaggagga
    16441 gggattcgaa ccctcgatac cccgttgaga ggtataacac cttagcaggg tgccgccttc
    16501 aaccactcgg ccactcctcc cgatcacagg agtatatcaa agacgtgttg cgcgttcgtc
    16561 tctcaccctt ctcctatctt tcgttgtcgt tggactcccc gtcgatgatg tcgggcttgt
    16621 cgttggcagg ctcggcgtcg atgatctgct cgcctgagac ctcttctttt atcctgacga
    16681 gcgtggcgtt gagggtggcg ccgaagagga tcgtgaggga ggagatgtag aggtagagga
    16741 gcaggatgat aacggtccct atcgagccgt aggtcctgtt gtaggtattg aagtcgctgc
    16801 tcagatacag gttgaaggcg acgctggcca gcacccagag caggatgccg acgacgccgc
    16861 ccggtgtgat ccagcggaac ggctggttgg cgtcgggggc gaagtagaac aggagtgcta
    16921 ccgtcaccac catgaacaag agcgccgctg gccagcgcaa tatgttccag acgaacatga
    16981 actcatcgcc gagagtaagt acctcggcga gcgtccttcc gatggagggg ccggcgatca
    17041 ggagaagtac tccgatcagt atgagcgccg agaggccgaa cgtcataagg atggagatcc
    17101 ccctgacctt ccagaaaggg cgtgtctcct gcacgtcgta ggcacggttg agggcgttca
    17161 tgagggccgc gaaggcgcca gaggcggccc agatcgtccc gaggatgccg acggatagga
    17221 ggccaggtgc cgtgtccgcg ttgcggagcg tgttctcggt gtaggtctgg atcaggccga
    17281 agacctgcgc cggcaggagc cgctcgaggt agacgaagag ctccgtagcg aactgcgggc
    17341 ttgcgctgga gaaggcgtcg aggatcgcca cgcagaacag tatgaagggg aagatggcaa
    17401 ggatcaggta gaaggcgagc tgcgcgccga gaccgagggc gtcgtcgcgc ataaactcct
    17461 ttatggttgc tttgagcgtc tcgtagcgga gaagatctct gaggtcgccg aagaagttgc
    17521 ctagcgaccc gcgcgctggt ctctgggcga cgtccctaag cttcgtctcg gccgtgtctg
    17581 attccatccg ggcgtgcccc tcttttcgta accggtcaga ttttaaccgc aatcccgatt
    17641 ctggacggcg aagaagccaa tccggtaagc ccgacacgtc gtataaaacg ctgtatggaa
    17701 gggttggtgt cgtgcagtgc gttacgtctc gcgccggatc actggcgcgg atgggatatc
    17761 atctgttccg aggggaccgg agggtccgtt ctcgccggcg gtcggggaag gctctggatg
    17821 gaccgtgtaa aagacgaggt tctggtgcaa agggtggcat ccggagacga acgcgccttc
    17881 tctggtctct acgaccgcta cgccggactg gtctacggta cggggttgcg ttacctggga
    17941 gacaggggga ccgctgagga gctggttcag gacgtcttca cctcggtgtg gaagagcgcg
    18001 ggcgggttcg accccgcgcg ggcgagcttt gcgacgtggg tgtaccgtat cacccgcaac
    18061 cgggcgacgg acctgatccg gcgccgcaag gcgcgcgtca ggaccgtcga gggcgaaccg
    18121 ctcttcgaac cgggagagac ggatccttcg ggcgatctct cgcgcaactt cgacgtggca
    18181 gccgcgctct cgcggctgac tccggtccat agggaggtgc tcgtcctcgc ctacttccac
    18241 ggcttctcgc agcgggagat ctccgatcgt acggctacgc ctcttggtac ggtaaagagc
    18301 cgcaccacgg cggcgctgcg cgccttgcgc gagaccatgg gcttcgacga ggatcggttc
    18361 tccgctgatg agtgagcacc ccgacacgag ggatctcctc ggaccctacg tgatgggcac
    18421 cctggaacct tacgagaacc gggaggtcga ggatcacctg caaggatgcg cgagttgcag
    18481 ggaggaggcg caagacctac ggctggccca cgaacgcctc gccgaccttg cgtacggcac
    18541 cacggcgcca ccggaggatc tcgaggccag ggtcttggca gggataccgc gacgtcgagc
    18601 aggtcgacgc gttccgtcct gggtcgctgc ggtcgccgcg gccttctgcg tgctcgcggt
    18661 aataaccgcc ttcaatctct tagaggacca ggccgcggcg accctgaacc ccaccggccg
    18721 cgcccccgat gccggcggcg aggtgagcct caggggcacc ggcgagaaca cggagctgcg
    18781 cctcgaagcg tggggactgc caccctgcaa gagcgaacag tactacgagc tgtggctcgt
    18841 cgagggcgat gagcgagtaa gtgcagggac ctttacggtc ggagagagcg gccgggtcga
    18901 cgtgacgctg aacgccccga acttcgccgc ctcttatccc agagtgggcg taactgccga
    18961 gtacgacaaa gacccccgcg ccagcgacgc gaagatgcta tcgggcgagt tgcacgatct
    19021 ctgaaagcgg tttcaggaag tccttcctga aaccgctttc aagccggtca gccctcagct
    19081 atcagcggtc agcaagaaca aagagctgaa agctgctgac tgcttgcaaa gaggtacatc
    19141 ctctctacat gttgcgcttg cgtgcctggc ctccgttggg caacaagggc aggataaagg
    19201 acatgcgggt tccctctccc aaccggctct cggcctcgat tcgaccgccg tgggcttcgg
    19261 ctatggtctg tgctatcgag agaccgagac cgctgcccgg ctccccgact tcggccaggc
    19321 ggtagaagcg ctcgaagatg agtgggagtt cggtctccgg tatgcctgga cctcgatcct
    19381 cgacctcgat gcgcaactcc ccatcggtac aggcggaact gaggattacg cattgtcctt
    19441 ccgggccgta cttgaccgcg ttatccacta gggccaggat cgcctgttct acccgcacag
    19501 ggtccaccct gacgagaccg gtcgccagga gcgtagtgtc gagggtcgcg tcgcgttctg
    19561 cggccagcgc cccggcccga cgctcgaggc ccgcgagcag ggcctgcgcg tccacgggca
    19621 ccatgcggaa aggcggggcg gccgactccg aacgggccag gaagagcagg tcctcgacca
    19681 tccgggacat cgtcgacgac tccctgacta tctcacggag gatctcctcg tgctcgcagt
    19741 gaccgtcgag ttcaagcccg acctccgcgt tgccccggat cacggtgaga ggggttctca
    19801 gctcgtggga gacctcggcg aggaactcgg ccctggcgcg cgcggctcta gcgagcgcct
    19861 cctcggcgcg cttgcgttcg ctgacgtcgc gcaaggccgt ctggaggcgg actactttac
    19921 cgctttcgtc gagtatcggc tccgtgaggg tctcgaacca ggtgtaatct cccgacactt
    19981 tgcggacacg gtaggttacc gagacggccc cctgtccatc gagcgccttc tcgtgggctt
    20041 cggagcggat gcgctccaga tcctcgggat gaaagagatc gtaggggtcc gtgccgagga
    20101 ggtcctccgg ctcgtagccg agcagacgcc gggaggaggg actgatgtag aggtagcgtc
    20161 cgtctggctc gtgcaggcag acgaggtcgc tggtcttctc cgcgagcagg cggaattgct
    20221 cctcgttctg tctgagcgcc tcctcagcca gcttgcgctc tgtaacgtcc cgcgcgatgc
    20281 cctcgatggc gatgacgttc ccagcctcgt cgtagatcgg cctgttgcgc tgttcggtcc
    20341 agatcagatg gccgtccttg tggtaccagc gcagcacgag gggctcttcc ggagagtgca
    20401 tagagttctg cagcaggtgc ctgtcttccg ggtggacccg cttgaggccg agatccgggt
    20461 ctgcgtagtg ctcctccgga ctgtagccgg tcatggctgt ggacgacggg cttacgtatt
    20521 cgaagcccgg cgtcgggtgg aagcggaggc ggtagatgag atctctcgca ttgtcagcgt
    20581 gaaaccggaa acgctcgtcg tcctcgcgcg acgcatccgg tcttcgcaac atgcccgcgc
    20641 gaagtgctct caacccccta aggccagacc ggccagcata cctccggcga ggatgctgcg
    20701 aaaccggcgg agctctgtgg gaagcctcac gggacggccc tccgaagaac acgtgactcg
    20761 ctcgtatcga aatggattat actggcgaca gggaaaggta gagatgctga tcctgatcgt
    20821 cgaggacgag gccgcgatcg cgcgtttcct gaagcgcggt ctgaacgccc agggccacag
    20881 ggtcgtctgc gcgtacgacg gagaagaagg cacgcggctg gccgtggacg aaccggtgga
    20941 cctggtcctc ctggatatct cgctgccccg cctggacggc cgccaggtgc taaggcgtat
    21001 ccgtcttcgt cggcccaagc ttcctgttct gatgctcacg gcccgcgacg acacgcgcaa
    21061 caaggtgggc gccctcgacg caggggccga cgactacctg accaagcctt tcgtgttcga
    21121 tgagctgctg gcgcgcgtgc gcgcgctcat tcgtcgctcg gaccaagccg agtcggtatc
    21181 gatacagacc gacggcctca ccataaacct cctctcgcgc cacgcctggc gcggagacga
    21241 gaggatagag ctctccagca gggagttcgc cctgctggaa tactttatgc gccacggcgg
    21301 gcaggtcctc tccaggcagc agatcctctc cgccatctgg gactatgact tcgagcccgc
    21361 ctccaacgtc gtcgacgtgt acgtgcgcca ccttcgcaac aaggtcgatc ctcccgacgg
    21421 gccttccctg atccagaccg tccgcggcgc cggttaccgc ttcaacgccg catcaaaccc
    21481 ttaacaatac accgcgtcta ccagaaaact tcaagatgaa agaaatttca tctccacttc
    21541 atcgtggttt cacacaccgt gtatacaatt ttgccatgtg gactacgggc ggtgatcaac
    21601 ctacccaatt tcgtgtcacc gctaccggtg gtcgaccatt acggggtccg gacgcgggcg
    21661 attcttcctt tccctactct tttcccatcg catcttcgcc ccccggactc ccatctggag
    21721 gtcgcgcgct ttggtgtatc tgtccgtcga agttggtggg gagatagcca agcgaggggt
    21781 tcgggccgtt cggggctcgg gagtaggttc gcagcgctct tgatcagttc ttcgtttgtc
    21841 ggtccggaca atctcatctg gcgatcatcc tatctgtacg tatactcccc tgtatgaggc
    21901 tcattgtcgc cgaaaagccg tcagttggcc gggacatagc tagcgccctg gggaggcatc
    21961 gaagggagaa aggatcgctg gtcggccaag actggaccgt tacgtgggcg ctcgggcacc
    22021 tggtggagct ggcgccgccc gacgcctacg gcgaggaata caagaagtgg cgtctggaga
    22081 gcctgccgat cctgccgggg cgcttcaagg tcagggtgaa ccacaagacc cgcgagcagt
    22141 tcaacgtggt gaaggggctg atgaagagca cttccgtgac ggaggtcgtc aatgcctgcg
    22201 acgcgggcag ggagggggag cttatcttcg cctaccttta tggtctctcg ggttgcaaga
    22261 agcctgtgct gcggctctgg atctcctccc tcacccgcga agcgatccgg gatggattcc
    22321 acgccctgcg tgagggcgct gcgatgaaac ccctcgaaga cgcggcccgc agccgcgggg
    22381 aggctgattg gatcgtcggc atgaacgcca cccgcgccta ctcggtccgc ttcagccgtc
    22441 ctggcaacgt actctctgtg ggccgtgtgc agaccccgac cctgaagctc ctcgtcgacc
    22501 gtgagaggga gatacaagac ttcaagcccg agaagttctg gaccgtccac gcccgctttg
    22561 cgcgggacgg gagtacttac gacggcgtat ggttcaagaa gaaagagaac cgcctgaccg
    22621 agagggaggc cgcggaccgg atagccaaga aggtgcgcgg cgggacgggg accgtcagca
    22681 aggcgcagaa gaagacggcg acggagaagc caccgctcct ctatgacctg accgagctcc
    22741 agcgcaacgc caacgcccgc tacggcttca cggccgagcg cacgttgcgg gccgcccagt
    22801 cactctacga agagagaaag ctgatcacct acccgcgcac ctcgagccgg tatctctccg
    22861 gggacctggt gggtggcctg aagaagcgga tcgaggcagc gggagcactc cccgaccttg
    22921 cgccattcgc ggagaagctc ctcggaggag agaagctccc gatcagcagg cgcatcgtgg
    22981 acgactccaa ggtcaccgac caccacgcga tcgttccgac ggacaagaga tcatcggggg
    23041 ggctgtcgcc ggacgagacg aagatctacg acctggtggc caggcgtttt gtggccgtgt
    23101 tcttcccagc ggcacgcttc gagaacacga ccgtcgtgac cgaggtcaga ggggagacgt
    23161 tcctttctcg tgggcgcgtc gtgctcgagg cggggtggcg ggcgctctac ccgggcgggg
    23221 taggagggcg gaaagagaaa gagccgcccg tgttgccacc gatcgaggtg gggcaggagt
    23281 gggatgtggc gaaggtcggc gtcaaagagg gagagaccaa gccacctccg cgctactcgg
    23341 agtcagcgct gcttggggcg atggagacgg cgggcaagct cgtcgaggac gaggagctca
    23401 ggcagcagat gaaggaatcg ggcctcggta cgccggctac cagggccgcc actatcgaac
    23461 ggctcataaa ggtaggctac atcgagcgcg agaagaagat cctcgtcccc acggagaaag
    23521 gaaggtcgct tatcgggctc ctcggggaga gcccgctctc ttccccggag ctcacggccc
    23581 gctgggagga gcggctcgcg aagatggaaa aaggcgccga gcgccgcccc gactttatgg
    23641 ccgacataaa cggcttcacc accaagctcg tccaagaagt ccgtgggatg gaaggagaga
    23701 ggctggcggc tccctcccga agtcgcgagc cgctcggcgt ttgccccaag tgcggtgctc
    23761 ccgtcatcga gacgaagaaa gcctacggtt gttcggcgtg gaagaagagc ggatgcgact
    23821 tcgcgatctg gaaacaggtc tccggcaaac gtctcagcgt aagccaggcc aaacagcttc
    23881 ttacgaaggg acacaccgca aagatgaaag gtttcaagag caaggcaggc aaaccctacg
    23941 ccgccgcgct caagctcgac ggagagcaca aagtacggct ggagttcgag agcagccgca
    24001 agtagagcct cctcgcgtca aagcgataac tgtttcttct gagttgtgcg ttacactctt
    24061 gcgggtatga aaactatcat cgtcggggcc ggattggctg gcctcacctg cgcaaaggta
    24121 ctcagagaac gcggaacgga ggtcgccgtc ttcgaggcgt cagaaggcgt cggcggtcgc
    24181 gtgcggaccg acgagcagga cgggtttctg ctcgaccggg gcttccaggt gttcttcaca
    24241 tcgtatcccg ttgcggggag gcaccttgac tacggggcgc tcgagttcag ggatttcgac
    24301 cccggtgcga tcgtccgcag gggagcggag gagagcgtcc tgtccgaccc cctgcgcgac
    24361 ccgagggcgc tcgtcccgac acttctctcc gacgcggcca ccctcgggga taagctccgt
    24421 acgctcgaga tggtggcgag aacatccacg gtggggatca gtgcgggtac ggaggatgga
    24481 gaggcagaca tctcgacgct cgagtacctg aagatggcag gtctctcggg gaggtacata
    24541 gactctttct tcaggccgtt ctacggcggc ataacgctga accgcgggtt gaccaccagc
    24601 gcccgcgtcc tccgcttcac cctgaggatg ctcgctgagg gcagcacggt agtccctgct
    24661 ctggggatgg gtgagatccc acgccaactt gcttcccatc tacgagatgg tgagatacaa
    24721 ctcgacagcc ctgtggaagc cctcctgcgc gaagggggac gggtggtcgg tatcaggagc
    24781 gtagggaaag agcacgaggc cgacgcggtg gtcgtggcca cggacgcgcc gacggccgga
    24841 gcactcaccg gtgcagcggt gcccgagggg actgtggggg aggtatgttt gtactatgag
    24901 acggacggtc tgggtactgg aaagaagata ctcttgaacg ccgaggacgg agcattcgtg
    24961 aacaacgccg tggagattag caatatctcc gagaagtacg caccgccgga tcgccacctg
    25021 ctctacgccg tggcgcttac gggaacgaac ctgcccgatg aggacctcta ccgcaagggc
    25081 atcgaggacc tgagccgctg gtatccccag gcggacttca ggcctctcgc gctacggcgc
    25141 atcccttatg gacagtttgc ccagccgcct ggcattcaca gcaggctgcc ggcgaacacg
    25201 acacagacgc cggggctcgt gctagcgggc gagtacacgg aggattcttc cataaacggt
    25261 tccatgctct ccggagagaa ggctgcgggg gcggtgatcg cgtgagcgcc aacggcactc
    25321 cggcgctaaa gatcgagaag ctcacgaaga cctactccaa cggatttctg gcgcttagcg
    25381 ggatctcgct cgaggtcgaa gcaggcaagt tcttcgggtt gctggggccg aacggagcag
    25441 gaaagacgac cctcataaac ggcgtggtca gcctggccag gccagattct ggctcggtcg
    25501 aggtcttcgg gaaggatgcc tatcgagagt ttagggaggc gcggcgtatg atcggagtct
    25561 ctccccagga agtgaacctg gacaagttcc tgaccgtcga ggagacgctg ttgttccacg
    25621 ccggatacta cggcgttccc aggaagaagg cccgccagaa ggctgaggag cttctggagc
    25681 agttcgagct cacggggaag cgtgggcaga ggacgaacac gctctcgggg gggatgaagc
    25741 ggcgcgttct gttcgccagg gcgctcatgc acgacccgaa ggtgctgttt ctggacgagc
    25801 cgacggcggg agtagacgtg gagttacgct acaagctgtg gggctacata agagaactga
    25861 accgcggggg gatgaccatc ctcttgacga cgcactatct ggaagaggcc gaggcgctgt
    25921 gcgaagagat cgcactgata aatgggggct ccatcgccgc ccaggggagc agcgaagagc
    25981 tcaaggagcg ctacgaggca gggaacgtcg aggaagtcta catgaaggtg ataggccatg
    26041 atcttcactg acaggacctt ccagacccta ctctcgcgcg agatactacg cttcacgcgg
    26101 gtctggatgc agacgatagt tcctcagatt ttaacgtcgc tcctctacct cgtggtcttc
    26161 ggcatcgcac tcggcggccg tataaagcag atcgaaggca tcccttacct cgagtatata
    26221 ttgccaggca tcgcgctgat gagcctcata acgaactcgc agatgaactc gtcctggtcg
    26281 gtcttcgacg ccaagcgcga gcgctacata gatgaggtgc tcatcagtcc tatgagcgac
    26341 ctgcagatcg cactcgccta ctctttgggc ggtatgctgc gcggggtggt gatgggggcc
    26401 gctgtcttcg tagtcggggt tcctttcgtc ggcgtgcaga tcaaacaccc cttgatgctc
    26461 gtgatgatag ggcttctcgc ttccttcgcg ttctcctctt taggcacgat ggtcggcgcc
    26521 ctggcgacga gggtggagca catctcgctc attacgagcg tcattatcca gcccctcgcg
    26581 ttcctcggag gggtgttcta ctccgtaggt atgttgcccg gcatactcaa ggtggtgacg
    26641 ttcttcaacc ctatcttcta caccgtcgac gcggcccgct acgtgacgct gggggtctcg
    26701 gacctgaacc cctacccgac cctgggcatg gtctgcctca tagccgccat ctgctttggg
    26761 ggtgcctggt gggccataag ccgcggcccg aatctgcgct actgagacgg cccgggtagg
    26821 tggcttccca agctgcgtac cttcgattcg acgcgctgaa cacgatccgg atggtacttg
    26881 taggaccagg aggtgtgcca agcgtcctgt tcgaggatag agaaacctat cgaccccacg
    26941 gccgcctgct ggcttccagc accaggagcg ctaccagggc tatctcgatg accttgtcga
    27001 cgtacccgat agcgttccgc gccccgacga gaatccagag gaagatcgtc agcgccgtat
    27061 agccgacgag gacccagcat accaggttac ggtactgtga gatgcggggt atcggcaagt
    27121 tcagggccgc gaggagcgta agatagccca acccgttgag aataaagagc gggaagccct
    27181 ggaggccgag gtagaggtgg atcagggctg tcccgacggt cagcacgatc acaccgaccc
    27241 ttagcggggc gaagtccgta gaggccatgt ccttcctcct ttccagcttg ctcctaccct
    27301 gatatcttcg ccctctgtac gagcctggtc cttcgccctg gcagtagatc cgccgcagag
    27361 gctatcaggg cgaggataat aagcgccccg ccgatgacgt aaggcgcagg gatgccgaga
    27421 tggtcgaagg cgagcccggc gagcgcgggg ccgaagatca tggcgaggct gaggatcgcc
    27481 tgcatgcctc ccatgagcct gccctgcgtc tcggccggcg tccgattgga gatgatggca
    27541 gtgagactag ggatggcgag ccccatccct agcgccagga ggccgacgat cgggtagagg
    27601 aggatgccgg aagggacgag tgcgacgagg aggaggttga gggaggccag gacgagtccg
    27661 ccgaggagga gcctgctctc cccgaagcgc ggctggaggc gtccgatcag gaccccctgt
    27721 gtcacgacgg cacacaatcc cacaaaggcg aagaagaaag cattcgaagt cgtcccccac
    27781 ccgaacctgg cctggctgaa gagcgggaag ttattcacta gcccggccag agccaggttc
    27841 agcagcagca ccgcggcgag caatgctcga acgtttctca tcttgagtat gcgggccagc
    27901 tgcgagatgg ggtcgagacg caggaggggg accttcttgc gcatgtgggg cgcatgggat
    27961 tcgggtaggg tcaggtaacc gaatacgcaa ttggcgaggg ccagtgctga ggcgaagagc
    28021 gctggggcgc tcaaggagtg gaggctgagg aggccaccga tggccggtcc tgccaagagg
    28081 ccgagtccga aggccgcccc gatcatgccc agaccccggg ctctgtcctc cctggccgtg
    28141 ctgtcggcta tgtaggcctg ggcggtcgcc agcgtgccgc tcgcggcgcc cgccaggacg
    28201 acggcgaaga cgagatagac gagcgtctgg gcgaggccga gcaagaggta agcgagcgag
    28261 gtcccgagca aacaactcag gagtacaggg cgtctcccgt ggcggtccga gagcccgccg
    28321 aggaaaggac cgctggcgaa ctgcatcgcg gcgtagagag aaccgagcag gccgacgagt
    28381 accgcgccgg aagcgtaagg gccagcgtag aagggcagca agggtaccac gatgccgtac
    28441 ccgatcatgt ccacgaacac cgtgacgaac aggaacacga gcggcgaccg gcgccgccac
    28501 acgtctgagt tcacatcggc ttcctgctct ccatcagccg tcgcgtctcc tcgatcacag
    28561 catcgccgac ggatcccgct tcggcatgaa tgggactccc gaactccacc ctggcgtcca
    28621 cgttgcgcag cgccgggacg agcacctgga gcgtggcggc gagccactcg cggtctcgcc
    28681 tgcggcggcg caggtggatc agggggttgc gcagggcaga aggtgagatc acaccgctga
    28741 ccacgacggg gacgaccaca agccctggca cgaggcgggc gaagaggtcg ccgctcgcag
    28801 accagcgatt aagggcctcg acggcgccgg gcaagattgc ggggtcgggc tcgattctac
    28861 cccccggaaa cgtgagtacg gtgccgccac cccgcaggtg cctggccgcc gcccgtacta
    28921 ccccagggcg tcccgcagag gtctcagcca cggtcagaag gtgacgcgaa gtgttcggga
    28981 gagcctctag aaagggtcgg tcggcggcga tcacaagcag gtcgtccctc ggcgttgccg
    29041 cgaagagggc gacggcgtct gcgaggccgg gatggttgga gaccaggagc agcggtccac
    29101 cgcgcggtac attcttctgt ccctcgatgc tggtgttgcg ggagagtctt ttcagcgccc
    29161 aggcgccacc cgtccccagg ccagactcgc cgactattct gtcgtaggtc aggatctggc
    29221 gtgccagacg ccgggctggt atgcgcgaga tagattccat tagcgcgcgt ccgtggctcg
    29281 ttccgcccag accgaaggca cagataaggt cttccacgca tagctcggtg agacgttcga
    29341 gaaggtcgtc gctccccgaa gaagcgtgaa actcctcggt cgcagtcggc aggtgggaga
    29401 tgctatccac ggtgcttcct cgtgacctct gtggtcacca cctgtgccac cctttcgctt
    29461 gcctcaaacg atgctcgggc acctcatccg ttagtacgca cgggccgcag gtgcggttcg
    29521 ggtaccacta aaaggatacc ccatggatcg cgcgtctagg ttcccggttc gcggcggcgc
    29581 aggatctcga cggccgagcc ggcgagcacg gtatcgtcga gcctgacgtg aggcttggcc
    29641 accttcacct ttacggcctc gaccgcggga tgctcgtcga gagtagccgc ggcgatcctc
    29701 tctgccaggg tctcggtaag gtgcacgggc agtccctcga cgatgtcacg cgccgcgcga
    29761 tggacctcac tatagtccac ggtcttcgtc aggtcatcag aggttcccgc tcctcgcaag
    29821 tcgaggcgca actcgatgct tacgatgaaa ggctggccaa gctcgtgctc ggcctgcagg
    29881 gttccgtggc ggccgtggaa gaccatgtcc gtaagaagta tgcgatcctc gctcatcgac
    29941 tcctttcggc ccattccatc ctaataagta tgccagaacg tgaagaccgg tatgccgggg
    30001 atcagcctga aaggcggctg tagtagaatc tctactctac tcgaccacga cccagatcgg
    30061 acacaaggat catgttccgg ccttggtctt caagaggacc atctgacata aagctcgccg
    30121 gtggcccgac gaacggagca tcgtgatagg agaggaacag tatccttcgg ccggcaaagg
    30181 ggcgtcgatc cggccttccg ttactgtgag ctacgcgcag acgctcgacg ggcgcgtggc
    30241 gaccaggacg ggggaatcga agtggatcag ttgccctgac tccctgcgct tcgcccacga
    30301 gatgcgcgct tctcacgacg ccgtcctcgt cggcgccgga acggtctgca aagatgatcc
    30361 ccgtctgacg gttcgccacg tccctggcac gagccccctt cgcgtagtcg tcgacagcac
    30421 cttgcgcacg cctccagacg ccgcggtgct cgccgacggc gcagcccccg gaacagtgtt
    30481 cgccgttacg gaccgcgcgc cggaagaaag tctcaagaag atccattctc ttggcgctac
    30541 cgtcataaag ttgcctcctg atccaggcgg ccgtgtcgag ttggcggtac tcctcgagga
    30601 gttggcagcg tcgggtgtgg aatcggttat ggtcgaggga ggggccacct tgatcacggg
    30661 ctttttcagg gccaggctgg tcgaccggat tgcggtgtgc gtcgcgccca agatccttgg
    30721 tgtaggcata gaagccatcg gcgacctcgg catccgcgac ctgtcccggt ctctcaccct
    30781 cgccgacacc gcggtggaac cttacggtgt ggatcttatc ctcaggggac gtatcgaata
    30841 tcccggtggt gccccggatc ggggttgaaa tagaggccag ggccctgtgg tttaccgctc
    30901 cacgcaccgc tgcgctccgt gcggagacgg tacgacctcc cggtccccag gaggtacgtg
    30961 tcgagaccat cgcctcggcc gtgagcgcgg gcacggagat gctggtcttc cgagacgagg
    31021 taccgcacga actcccgctg gacctgccca ccctcgcagg gagctacgca ttcccgatca
    31081 aatacggcta cgccgccgcg gggcgtgtcc tcgacactgg ttctggcgtc gaatacctct
    31141 ctccggggga tcccgtcttc gtccaccatc cacaccagga ggtcttcgtc gtccccgcac
    31201 agatgcccgt gcgactcccc gacgagctcg atcccgtact cggcgtattc agcgccaacc
    31261 tcgagaccgc cctgaacatc gtacacgaca cacccgtgcg gctaggtgag acggccctgg
    31321 tcttcggtca gggcgtcgtc ggcttcctcg tagccctgct cctgaagctc tccggcgcag
    31381 gatcggtact cgtggtcgat cctctagagg agcgccgcaa actggcactc gccgcgggag
    31441 cagacgtggc gttcgcgcca ggagggttga atgatcgtgt catggaaacc acgggcgggc
    31501 ggggggctga cgttgcggtc gagaccagcg gggccggggc tgctctgcag agcgcgatag
    31561 acgccgtggc gaccgagggg acggtcgttg tagcttcgtg gtacggcaca aagcctgtca
    31621 cgctcgagct cgggggacat ttccataggg gaagggtgag gctccgttcc tcgcaggtcg
    31681 gtcgtataaa cgcggagctt gcgccgcgtt gggacagggc ccggcgtatg gagacggttc
    31741 tcggcctgct gggccggctg gagttgaagg agctcatctc ccaccgtatc ccgttcgaag
    31801 aggcgcctga ggcctatacg ctcctcgaag agaggccaca agaggcgctg caggtgatct
    31861 ttacctacga gggagtcaga ggaggccagg atgtatgaaa tctacgtcgc cacgaggttc
    31921 gaggctgccc acaggctcat cggggacttc gggcccgcga ccaggacgca cggacacacc
    31981 tacaggatgg aggtgatctt gcgcggccag cgtctcggag acgatggcac gctctacgac
    32041 atcggcgagc tggggcaggc cgtaggggag ctggccggct ccatgcacta cagggatctg
    32101 aacgaggtcc ccggcctgca ggaagtcaac acgacggccg aggcggtggc ctcctactgt
    32161 tgggagaaac tggcagaacc cctccgcggc cagggcctcg actcgcttac ggtacgtatc
    32221 tgggagtcct ccgacgccta cgcggcacgc gaagacacgc tggactgagc gatttcgcgt
    32281 gcgcgtcgcc ttcatcacgg tgggggacac aggccgcaag accggcggct acctgtacaa
    32341 cggacgtgtg atctctggcc tgcgcaagag ggggttcgag atcgaggagt tcgtggccgg
    32401 tcgagcatcg cccgacgagc aacgaaaggc cgccccaaga ttcggaacta cgttcgaccc
    32461 ttcggggtac gacgcgatag tagtcgacgc cctcgcccgc atcgcggtcg caccgcacct
    32521 ggacctttgg ctgtcgcggc ggcccgtcgt tgcactggtt cacgaactgc ctagcctcgc
    32581 cggcggcggg tccggacctg agactatagc ctgcgagcgc gactacgagg agccgttgct
    32641 gcgcgcgcac cggctcgtcg ccgtgagtaa caatggcaga aacgtgctcc tgggacgggg
    32701 tgtctcgtcc gggcgtatcc gtgtcgtgcc gcccggcttc gacggcattc ctgagggcgt
    32761 tggccctcac gttcagggca gcggtcccgt gcgcgcactc tgcgttgcgc agtggataga
    32821 gcgcaagggg atactgaccc tggtcgaagc gtgggcgctg cgcgagcgta agggcgcggt
    32881 gctagagctg atcggggaga cggacgctga ccccgattac gccgtcctgg tcggggacgc
    32941 catcgaagcc gcaccacggg gctccatcat cgtatccgga tgtgtggaca acgcagcgct
    33001 cggtgcctcc tacacggggg ccgacttctt cattctgcct tcgcgctacg agggatacgg
    33061 gatcgtctac gccgaggcgc tcgcctgcgg gctcccgatc attgcctgcg atattggtcc
    33121 tgtacccgac ctcgtcggtc gggaggccgc ggtgctgatc cggcccgacg ataaggagga
    33181 actctctgct gccctggacc tccttatcgg ggattccgca ctcagggcga gcatgtccgc
    33241 cgccgcaatc cgccgggcca gacgcctacc gcgttgggaa gataccgtca gcggcttcga
    33301 acaggttctg aggaccgcgg ccgagaccgg gaagcaggga cccatgtaga atctgtcgat
    33361 ggaccgcaga gaccttcgag aacagaaccg ggtgtcctgg aacgccgtcg ttcgtgccca
    33421 cgacagccac cgcggggacc tttcgcggtt cctccgtggg ggtggcagta cgcttttccg
    33481 ggaggaacac gatcttctcg gtgagctcga gggaaagtcc ctcgtccacc tccagtgcaa
    33541 ctcggggggc gactcgataa gcctggcgag actcggcgct acggtcacgg gagtggatct
    33601 aagtgacgaa gccgtatcat cagcccgaga tctcgccgat aaaaccggca taagggtcgc
    33661 gttcgagcgc gccgacgtat acgactggct gcagaaagca gcacgcgaag gcagggtctt
    33721 cgacgtagcc ttcgcctcct acggtgtcat ctgctggctg cccgacctca gaagctgggc
    33781 ccagggtata gccggcgtcc tgaaccccgg cggacgcttc gtgctggtcg acttccaccc
    33841 tgcggccgac gtcttcgaca aagactggaa ccacgtccac gactacccct caggcggtgt
    33901 cccgctactc gtggaagaag gcgtcgacga ctacgtggcc gcttcgggcg gcggtctcac
    33961 cccagccggg ttcgtcgagg gagtccgaga cttcgagaat cccgaaggtt gctatctctt
    34021 tcggtggagg ctcggagagg tgattacggc gctggccgaa gcaggactga gcatcaccgc
    34081 tctcgaagaa tacccttacg cgaacggaga aagaaagttc gccggtatgc gcgagctacc
    34141 aggaaagcga atggtcccgc cggaccatat accggcgatc ccgctgatgt acggcatccg
    34201 ggccgaaaaa gctgaaagct gagggctgaa agctaacagc tattcatggc gagaagaaga
    34261 acgcgagggc ggcgatgatg tagacgccgc agagcatcgc gccttccagc cagttggatc
    34321 tgccgtcgag ggcggcgaag cccgttacga ccacggcggc cccgagggcg ccgagttcca
    34381 ggggggtgaa gatcaggcgc aggggatggc cgagcagggg accgaggaag acgaggatgg
    34441 gggtgacgag cagggcgacc tggatggagg agcccaaaga gatgcccatc gagaagtcca
    34501 tgtcgttctt ataggcgatc tgtacgccca cgacgtgctc ggcgatgttg cccacgatcg
    34561 ggacgaggat gaccccgacg aagagctcgg agatcccgta ctcctcggtc agtggctcta
    34621 tggcgccgac gaaagcctcc gagacgtagg cgacggccgc cgtggccacg aacagcagtg
    34681 ccagcgaagc cgtacggccg aacggggcgt ggccctcgcc ctgcgcggcg cctcctgggc
    34741 tacggaggat gtagaccagg taaaggacgt agagcacgaa cataaccacg gccacctcga
    34801 tgctcaggga ggtggggcta ggatcggggc ttgtggccgc gaatatggtc ggcaaagaga
    34861 gcgccgcggc cacgatggcc agcatcgagg cgttgatgcc tgcgatctcg gccgagaagt
    34921 gctggatgcc gttcttgaga cctccgaaga ggaggctcgc cccgagcacc aatagtacgt
    34981 tgccgatgat cgaaccgatg atggaagctt tcaccacggt cgtgaggcct gcggagagtg
    35041 cgaagatggt tatgatgagc tccgctgcgt tgccgaaggt ggcgttcagg atgccgccta
    35101 tacgcgggcc tgagtgatgg ccgaggctct cggtggcctg gccgaggacc caggccaggc
    35161 cgatcagggc cagggccgtg gcggcgaaga tcaggagcgt cggggcgtgt agaagctccg
    35221 cgaccaccac ggccaccgtg agcgccgccg ttacaccgta ggctaccttg gcgagcaatc
    35281 aaactccttc cacaatctcc tccacataaa catgaggagt atcttataag atgagaggac
    35341 ctgaagtaac gacttctgga gagtagcctt tgatcgacta ccacctgcac gtcatagccc
    35401 acggcgaccg tccgatgacc gtggagaaca tactgagcta ctgcgaggtc gctaaagagc
    35461 ggggcgtcag ccagatgggc atcaccgagc acgaccgcta cctcgaagag atagacctcg
    35521 ccgcttttca ggaggcccgt gagctctcgc aagacgtaga tctgcgactc gggatagaga
    35581 tagactacgt accaggagca gaggaacgga tggaaaacga cgcc
//
LOCUS       DATMVF010000284        15725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_454109, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000284 DATMVF010000000
VERSION     DATMVF010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454109"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VN178_14790"
     CDS             complement(<1..176)
                     /locus_tag="VN178_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS82284.1"
                     /translation="MRVVIAGGGLAGLTAALRATELGAQITLLEKGESPGGSFVYSSG
                     YIWSYVDLPTYRREA"
     gene            complement(187..882)
                     /locus_tag="VN178_14795"
     CDS             complement(187..882)
                     /locus_tag="VN178_14795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS82285.1"
                     /translation="MKVKRLDHIALYMGNRDVAAKFLTKHLGFHVVDHTERYTLVGAG
                     GRLGKLTLFDAPQGTTPSPEEIERITIRVADPEGAAARLPQEAGAEPRDGGYSFTGPE
                     GLPLALVPGEGEFADYDLDGFVLRSGSPEESARTFVEMGFAPGDDATTVKAGEYSMGL
                     TGSSPDANGGGMLFHVGCLVDSAEDHRKEAEERGLEVQDFVEGPNTLAVFVRGPEGVS
                     VEYVEHKSTFSLT"
     gene            1183..2358
                     /locus_tag="VN178_14800"
     CDS             1183..2358
                     /locus_tag="VN178_14800"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWS82286.1"
                     /translation="MERTVILGAARTPFGRFGGALSPLSAPELGGAAIRDAIDRSGIK
                     DEDLQHSIFGIVVQAGVGQIPSRQANFHAGLPFSLTTETLNQVCASGLRSATLAETLI
                     RAGDYDVILAGGMESMSNAPYLLPKARWGMRMGDMPSLDAMMHDGLLDAFEHVNMVRF
                     GTEGAKKFDISREEQDEWALRSHQRATAATDEGRLHEEIVGVKVPGKKGQMDYVEADE
                     PIRRDTTLEKLASLAPIDEGGTVTAGNAPGVTDGAGALVLSGERFAERRGLEPLGSIV
                     AHAKVAEEPPNLLTVPGNAGKLALQKAAWNADDLDLVEINEAFAGVAIHSTRVLGVDP
                     DIVNVNGGALALGHPIGASGARILTTLLYELRRRGGGKGLAGICSGGGQGDAVLVEV"
     gene            2391..2909
                     /locus_tag="VN178_14805"
     CDS             2391..2909
                     /locus_tag="VN178_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWS82287.1"
                     /translation="MLPHGDITPQIARSAWVAPGAFVIGDVHLGEESSVWYGAVLRGD
                     TEPIRIGARSNVQDGCILHADPGYPAVVGEGCVLGHNAVVHGCEIEDNCLVGMGATIL
                     NGAKIGEGSIIAAGAVVPEGREFPPQSLIVGLPAKRAGDVSEELAADIARGASEYVER
                     ARAHQESLGSGA"
     gene            complement(2906..7765)
                     /locus_tag="VN178_14810"
     CDS             complement(2906..7765)
                     /locus_tag="VN178_14810"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HWS82288.1"
                     /translation="MLVKDELLDKVVARIREQMPEDQAPQVEEFARQYYGWVDAADLE
                     DLSPIDAYGAALSHWSFAGRREVGEWKIRVYNPHFEEHGWQSTHTVVEMVNDDMPFLV
                     DSTRMEVNRQGYAIHMILHPVMNVRRDEEGRLLEVLPPDSDEEDAISESVIHVEVDRQ
                     TEPYVLEDLKQAIIKTLADVKAAVEDWPEMRGRVGEIASGLEENLPDFEPEDLAETRA
                     FLEWIDDNNFTFLGYREYDLITQNGEEALCAVQGTGLGILRQTESGPVSRSFAELPPG
                     VRRLARTPKLLNLTKANSRATVHRPSYLDYIGIKKFDESGEVTGERRFLGLYTFSAYS
                     ASAFDIPLVRRKVRYVLERAGFPEGSHNEKDLIEILETYPRDELFQISKEELFEIAMG
                     ILHLQERQRVRFFVRRDTYGRFFSCLVFVPRDRYNTVIRERMQDILLKAFDGANVEFN
                     VRLSESVLARIHFIIYTKPGDTPEYDEEQIEARIVETTRSWTDNLYDAMIEQCGEEQG
                     IELFRKYRDAFSPGYRAGFLPRTAVSDVQRMETLKSEDDLGMSLYHPIEEPEDFLGFK
                     LFRLGDQVSLSEILPLLEDMGVEVVDERPHEVKPAGSPPVWIYDFGLVHEADDELQTG
                     EVKEIFQEAFAGAWRGAVENDGFNRLVLRARLTWREITILRAYCKYLRQAGSTFSQNY
                     MEDALVNNHHIARLIVDLFEARLDPSRQNTAEAESERLKEEIEEALEEVVSLDEDRIL
                     RSFLDITLATLRTNYFQSTPDGDPKAHLSFKLDPQRIPGLPLPLPRFEIFVYSPRTEG
                     VHLRGGEVARGGIRWSDRREDFRTEILGLMKAQTVKNAVIVPVGAKGGFVVKRPPAEG
                     GREALQQEVVACYKTLIRGMLDVTDNISGDEIVPPPDVVRYDDDDPYLVVAADKGTAT
                     FSDIANGLSAEYGFWLGDAFASGGSVGYDHKEMGITARGAWESVSRHFRELGHDTQNE
                     DFTVVGIGDMSGDVFGNGMLLSRHIKLVGAFNHMHIFLDPDPDPEKSFQERERLFGLP
                     RSSWTDYDEDLLSEGGGIFPRTAKSIPLSPQVREMLDLEAESMTPNELINAMLKARVD
                     LLWNGGIGTYVKASFESNAEVGDRANDVLRVDGNELGCRVVGEGGNLGFTQMGRIEYA
                     LSGGRIYMDAVDNSAGVDCSDHEVNIKILLDAIVEAGDMTEKQRNELLASMTDEVGDL
                     VLRDNYQQTQAINQALALAYPMVDVHARYIHSLEHEGRLDRSLEFLPDEEELGERRSD
                     NKGLTAPELAILLSYSKITTYQDLLNSDAPEDPYLSREPEKYFPTPLRERFSEQIHEH
                     RLHRQITATHVTNSLINRNGPSFVFRLGEETGAAAPDIARAYTVAREIFDMRAMWDGI
                     EALDNTVGASVQTRMTLDARKLVERATRWLLRYRRPPLDIGATISHFSGGAAELSERI
                     PEILLEGDREAVENAAQRMIDANVPPDLAKRAANLGPMFSALDITDVANSTGESLDTT
                     AAVYFILGDRLRLHWLRRHIEALPRDNRWRTLARSALRDDIYNQQAALAAEVLTDTPD
                     DKPVEERIEEWVEANKGPAERTLQVLTDINSSGTFDLSTLSVALREIRNLITTPETPP
                     EEAKATART"
     gene            7987..9183
                     /locus_tag="VN178_14815"
     CDS             7987..9183
                     /locus_tag="VN178_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWS82289.1"
                     /translation="MNFDLGTDQQGIKNRAARFADREVAPRAPELDRDDVVPLETLDK
                     LADAGFMGLCVPKEYGGAGMDFLSYCLLIEEISRADAGVGVTLAVHTSAGTLPIVMFG
                     TEEQKSRWVPPLARAESIGCFALTEPETGSDASAIRTRAERVEDGYRISGHKQWVTNG
                     RVAGTMILFARTEGGARDGVTAFVVSMEAEGISFGKHAQKMGVISATTDDVLFDNVFV
                     PKENRLGEEGRGLRVALGTLDTGRMGVAAQAVGIAEAAFRYATQYAAGRTTFGKPIAE
                     HQAIAFKLADMQTKIRAARLLVYEAAWMKDQEMPHGEAGARAKLYASQVANEVAYEAL
                     QVLGGRGYMKDHPVERYYRDARVTEIYEGTSEIQRLVISRAILREHEESVGAPGSVDQ
                     SRPVVG"
     gene            9252..10895
                     /locus_tag="VN178_14820"
     CDS             9252..10895
                     /locus_tag="VN178_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWS82290.1"
                     /translation="MDTRKSAEDRWEEAYSKGPERDAEFSTMSGVPVKPLYTPDDVEG
                     AYDEKLGYPGEYPYTRGVYPNMYRGRLWTVRQFAGYGTAAETNARFRYLLDHGQNGLS
                     VAFDMPTLMGLDSDSPLSLGEVGTEGVAVDSLEDMERLFEGIPMGEVSTSMTINAPAM
                     VLLAMYVVAAEKQGVAPEELEGTNQNDILKEYIAQKEWLFPPEPSMRVFKDMLVYATE
                     HMPKWNTVSISGYHIREAGSTAAQELAFTLADGFAYIEAGIEAGLDVDDFAPRFSFFF
                     NSHIDFFEEICKFRAARRIWATVLKEKYGAKDERSLRMRFHTQTAGVSLTAQEPYNNV
                     ARTAFEALAAILGGTQSLHTNSLDEALALPTEEAVRIAVRTQQIAALETGVANTIDPL
                     GGSYFVEALTDELERQAYDYFRQIDEMGGMIQAIEQGFPMREISEASARYQRELEENK
                     RYMVNVNIYEPQDEQEMELHRIDPEVSKGQVERLKALKENRDNARVERCLEELKQACR
                     GDDNTMYSILEAVRAYATVGEISAAMQEVFGDYRETPEI"
     gene            11212..11526
                     /locus_tag="VN178_14825"
     CDS             11212..11526
                     /locus_tag="VN178_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82291.1"
                     /translation="MEARVVDLRILPTETREVVRIFRGSVLPVARRQQGFKGALLLTD
                     DETGVGISISLWETEEDREEGEANGFYQQQIRKFSELLTETPVRKHYNVGVLHYDASV
                     LA"
     gene            11646..12650
                     /locus_tag="VN178_14830"
     CDS             11646..12650
                     /locus_tag="VN178_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWS82292.1"
                     /translation="MAIRETSAGHEALGLDEDDLRRMHYYMLLARRVDERSWILNRQG
                     KAAFVISCQGQEAAQVGAAYNLRPGNDFLYPYYRDSGMTMLLGQTPRDQLLSLLGKKE
                     DPNSGGRQMPGHYSSRRLNIVTSSAPVGIQYPQAAGTALAYKIRGEDGVVLVCGGEAS
                     TSGGDWHEAMNFAGIHDLPLVFLVENNVYAISIHASFQVAGSIAKRAEGYGFPGHEVD
                     GNDVLAVYEAAREAFERARAGEGPTLIEAKTYRMTAHSSDDDDRRYREREEIEEWRQK
                     DPIVRFEKYLMENGVLDEAERDEISERIKAEVAEASKYAEDAPFADPEDVLSGVYAEA
                     "
     gene            12659..13645
                     /locus_tag="VN178_14835"
     CDS             12659..13645
                     /locus_tag="VN178_14835"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWS82293.1"
                     /translation="MATKNLLQAIHEGLAEEMRSDESVMVLGEDVGRAGGVFRVTQGL
                     QEEFGAARVMDTPLAESLIVGSAIGLSVNGMRPVAEIQFADFIPPAFDQIVNEAAKFH
                     YRSNGDWNCPITIRAPYGVVPGGALYHSQSVEAWFCNTPGLKVVAPTFPGDAKAMIKS
                     SIRDPNPVLFLEHKRTYRLIKEEVPDGDHEVPLGEARVHREGADITVVAYGLMLYMAL
                     EVAEKLSEEHGIETEVVEPRTLYPLNKEKILASAKKTGKVVVISEANLTGSVSGEIAA
                     IVAREAFEWLDGPVMRLGAPDVPAAAFAKPMMEHFLPSAEKIEEAMLELARY"
     gene            13657..14994
                     /locus_tag="VN178_14840"
     CDS             13657..14994
                     /locus_tag="VN178_14840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWS82294.1"
                     /translation="MAKPVTMPQLGESVTEGTITRWLKAEGDEVEIDEPLAEVDTDKV
                     NAELPSPVAGKIEKFLVSEGSTVDVGTEIALVAAEGDSAAVDAPPRENVADPAPTEEF
                     PASDTEAQPVAGEESPAARTSRSDGNGRGDVEDAETLRLTRSSPVVRRLAEEHGVEIA
                     EIPGTGTGGRVTRKDIEGYIEELEEREEYAPGLQEEREAFAEPEPEIERVPVYEGDRV
                     EDLTGIRRAIANRMSASKREAPHAWTMVEVDVSGLVALREREKTAFVEREGVKLTYLP
                     FIVKAVVESLKENPVLNSVWDGDRIILRKRINVGVAVDLEDALIVPVITDADELNVLG
                     LARKIEDLVRRARARNLGTEDVSGGTFTVNNPGSLGSVVSTPIINHPQAAILSAEAIV
                     KRPVVIEDAIAVRSMMNLEVSFDHRVLDGGAALKFLNAVKSRLEACGPESSIG"
     gene            15122..15343
                     /locus_tag="VN178_14845"
     CDS             15122..15343
                     /locus_tag="VN178_14845"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWS82295.1"
                     /translation="MKYLVVIEPTDTGFSSYSPDLPGCVSTGASREEVEQNMQEAIEL
                     HLEGLREEGYSVPEPSTSSSYAEVIQRLA"
     gene            complement(15482..>15725)
                     /locus_tag="VN178_14850"
     CDS             complement(15482..>15725)
                     /locus_tag="VN178_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWS82296.1"
                     /translation="ESPLVGVGVETVGIDAGGAAEFDPSFPVHFYLMGAGKYGVTQLA
                     NLGALPQTGALLFVAPLKLTGGTGSPVRALALVPQG"
BASE COUNT         2974 a         4870 c         5057 g         2824 t
ORIGIN      
        1 gcctccctgc ggtatgtagg caggtcaaca tacgaccaga tatagccgct ggagtacacg
       61 aaggatccgc cggggctctc acccttctcg agtaacgtta tctgcgcgcc gagttcagtg
      121 gccctcaggg cggctgtcag gccggctagc ccgccgccgg cgatgacgac gcgcaccggt
      181 gctaccctag gtcagggaga acgtagactt gtgctccacg tactccacgc tcacgccctc
      241 tggcccgcga acgaagacgg cgagggtgtt cgggccctct acgaaatcct gcacctcaag
      301 cccccgctcc tcggcctcct tgcggtggtc ctccgccgaa tctacgaggc aaccgacatg
      361 gaagagcatg cctcctccgt ttgcatcggg tgacgagccg gtgaggccca tgctgtactc
      421 ccccgctttc acggtggtgg cgtcgtctcc tggggcgaag cccatctcga cgaaggtgcg
      481 cgcggactcc tcgggggatc ccgaccgcag gacgaagcca tcgagatcgt agtccgcgaa
      541 ctccccttcg ccgggaacca gggcgagcgg gagcccttca gggcccgtga aagagtaccc
      601 tccatcgcgc ggttcggccc cggcctcttg cgggagtctc gctgcggcac cttccgggtc
      661 ggccacacgt atggttatgc gttcgatctc ctccggagaa ggcgtcgtac cctgcggggc
      721 gtcgaacaag gtgagtttgc cgagcctgcc accggccccc accagggtgt aacgttctgt
      781 gtggtccacg acgtggaacc cgaggtgctt ggtcagaaat ttggcggcaa catccctatt
      841 gcccatatag agtgctatgt gatccaacct ctttaccttc atggatcctc ctcttgatgt
      901 gctctgcaac cgaaaagata gcatgtaata cgtaaactag tagatcgcct ctggttgatt
      961 gtacgcagcg cccgccgcag tggattcccg cttgcgcggg tatgacggga gaggaactgg
     1021 ggtccatcac ctagcccttc ctaactcacg ataccagctt cacaattcga gagtaatctc
     1081 agatctgacc ctatatctgg gggcgcctga actacgaaac gcgcgcttct tcccaggaag
     1141 gcttagacta tggtcgttgg tcctccagac agaggagtcg cattggaacg cacggtgatt
     1201 ctcggggcgg cgcgcacacc cttcggcagg ttcggcggcg cattgtcccc tctgagcgct
     1261 ccggagctcg gtggggcggc gatcagggac gccatagacc gctccggcat caaagacgaa
     1321 gacctgcagc actccatctt cggtatcgtg gtgcaggccg gtgtggggca gatcccatcc
     1381 cgccaggcca acttccacgc ggggctccct ttctccctga ctacagagac cctgaaccag
     1441 gtttgcgcct ccgggctgcg gagcgcgacg ctcgcggaga ccctgatccg tgccggagat
     1501 tacgacgtga tcctggccgg tggtatggag agcatgtcga acgcccccta cctgttgccg
     1561 aaggcccgct ggggcatgcg catgggcgac atgccctcgc tcgatgccat gatgcacgac
     1621 ggcctgctcg acgccttcga acacgtgaac atggtccgtt tcggtaccga aggagccaaa
     1681 aagttcgata tctcccgcga agagcaggac gagtgggcgc tcaggagcca ccagagagcc
     1741 acggctgcga ccgacgaagg gcggcttcac gaagagatcg tcggcgtcaa agtccccggc
     1801 aagaaggggc agatggacta cgtcgaagcc gacgagccga tccggcgtga caccaccctg
     1861 gagaagctgg cgtctcttgc gcccattgac gaaggcggca ccgtgaccgc gggcaacgcc
     1921 cccggcgtca cggacggcgc cggcgccctc gtgctctcgg gcgagcgctt cgccgaaaga
     1981 cgtggcctcg aaccactcgg ttccatcgtg gcgcacgcca aagtagccga agaaccgccc
     2041 aacctgctca ccgttccggg caacgcaggc aagctcgcgc tccagaaagc agcttggaac
     2101 gcggacgacc tggacctggt cgagataaac gaggctttcg ccggcgtcgc gatccactcc
     2161 acgcgcgtac tgggcgtgga ccccgacatc gtcaacgtca acggcggcgc cctggcgctc
     2221 ggccacccca taggcgcctc aggcgcgcgc atcctgacga cgctcctcta cgagctgcgg
     2281 cgcagaggag gcggcaaagg cctcgcgggt atctgctccg gcggcggcca gggcgacgcc
     2341 gtgcttgtgg aagtatgagc ggcgaaagag atctgaacga aaggagcaat gtgcttccgc
     2401 acggcgacat cacgcctcag atcgcacgtt ctgcctgggt cgcaccggga gctttcgtca
     2461 tcggggacgt ccacctgggt gaagaatcga gcgtctggta cggggccgtg ttgcgcggcg
     2521 acacggagcc catccgtatc ggggccaggt ccaacgtcca ggacggctgc atcctccacg
     2581 ccgacccagg ctatcccgcg gtcgtcggcg agggatgcgt cctcggccac aacgcggtcg
     2641 tgcacgggtg cgagatcgag gacaactgtc tcgtagggat gggcgccacg atcctaaacg
     2701 gcgcgaagat cggcgagggc tccatcatcg ccgccggcgc cgtcgtcccc gagggccgcg
     2761 agtttccgcc ccagagccta atagtcggcc ttcccgccaa acgcgccggt gacgtgagcg
     2821 aggagctggc tgcggatata gcccgcggcg cgagcgagta cgtcgagcgc gccagggcgc
     2881 accaggagtc gttgggaagt ggggcctagg ttcgggcggt ggcttttgcc tcctccggtg
     2941 gggtttcggg ggtcgttatg aggtttcgga tctcccgcag cgcgaccgag agggtggaga
     3001 gatcgaaggt gccgctggaa tttatatcgg tgagaacctg cagggtgcgc tcggcgggtc
     3061 ctttgttggc ttcgacccat tcttctatgc gttcctcgac gggtttgtcg tccggggtgt
     3121 cggtcagcac ctccgcggcg agcgccgcct gctggttgta gatgtcgtca cgcagtgcag
     3181 agcgtgcgag cgtgcgccag cggttatcgc ggggaagcgc ctcgatgtgg cggcgcaacc
     3241 agtggagcct cagcctgtcg cccaggatga agtagacggc cgcggtcgta tccagggact
     3301 cacccgtgga gttggccacg tcggtgatat ccagcgcgga gaacatcggg cccaggttcg
     3361 cggctcgctt tgccagatcc ggcggcacgt tcgcgtctat catgcgctgg gcggcgttct
     3421 ccaccgcttc gcggtcgcct tctagcagga tctcggggat gcgttctgag agttccgcgg
     3481 cccctccgga gaagtgcgag atcgtcgccc cgatgtccag cggcgggcgt cggtagcgga
     3541 gcaaccagcg ggtggcgcgc tcgacgagct tgcgggcgtc gagcgtcatt ctggtctgta
     3601 cactcgcccc gaccgtattg tccagggctt cgatgccgtc ccacatggcg cgcatgtcga
     3661 agatctcacg cgccaccgtg taggcgcggg cgatgtcggg tgctgcggcc cccgtctctt
     3721 cgcccagcct gaacacgaag gaagggccgt tgcggttgat caggctgttc gtgacgtggg
     3781 tggccgtgat ctgccggtgc agcctgtgct cgtggatctg ctcggaaaac cgctctctca
     3841 agggtgtcgg gaagtacttt tccggctctc gcgagagata ggggtcctcg ggggcgtccg
     3901 agttcaggag atcctggtag gtggtgatct tgctgtagga caacagtatg gcgagctcgg
     3961 gagcagtaag ccccttgtta tcggagcgcc gctcgccgag ctcctcctcg tcgggcaaga
     4021 actcgaggct gcggtcgagc ctgccctcgt gctccaggga gtggatgtag cgggcgtgca
     4081 cgtcgaccat ggggtaggcg agggccaggg cctggtttat ggcctgggtc tgctggtagt
     4141 tgtcgcgcaa aacgaggtcc ccaacttcgt ctgtcatgct cgcgagcagc tcgttgcgct
     4201 gcttttcggt catgtcgccg gcctcgacga tggcgtcgag caggatcttg atgttcacct
     4261 cgtggtcgga gcagtcgaca cctgcggagt tgtcgacggc gtccatgtag atcctgccgc
     4321 ccgagagagc gtactctatg cgtcccatct gcgtgaaacc gaggttcccg ccctccccga
     4381 ccaccctgca tccgagctcg ttaccgtcta ccctgaggac gtcgttcgcc ctgtcgccta
     4441 cctcggcgtt gctctcgaaa ctggccttga cgtaggtccc tatgccgccg ttccacagca
     4501 ggtcgaccct ggccttgagc atcgcgttga tgagctcgtt cggcgtcatg gactccgcct
     4561 ctaggtcgag catctcgcgc acctgcgggg agagagggat cgacttggcc gtgcggggga
     4621 agatgccgcc gccctcggag agaagatcct cgtcgtaatc cgtccacgat gagcggggca
     4681 gcccgaagag gcgttcgcgt tcctggaagc tcttctccgg gtccgggtcg gggtcgagga
     4741 agatgtgcat gtggttgaag gcgcccacaa gctttatgtg gcggctgagg agcataccgt
     4801 tgccgaacac gtcgcccgac atgtccccga taccgaccac ggtaaagtct tcgttctggg
     4861 tgtcgtggcc gagctcccgg aagtgccggc tgacagattc ccaggccccc ctcgcagtta
     4921 tacccatctc cttgtggtcg tagccgacgg agccaccgga ggcgaaggcg tcgccgagcc
     4981 agaagccgta ctcggccgag aggccgttgg ctatatccga gaaggtcgcc gttcccttgt
     5041 cagccgcgac gacgaggtag gggtcgtcat cgtcgtagcg cacgacgtcg ggaggaggga
     5101 cgatctcatc gcccgagatg ttgtccgtaa catccagcat gccccggatc agggtcttgt
     5161 aacaggcgac gacctcctgc tgcagggcct cgcggcctcc ttcagcgggc gggcgtttta
     5221 ccacgaaacc acccttcgcc ccgacgggga ctattacggc gttcttcacc gtctgggcct
     5281 tcatgaggcc caggatctcg gtcctgaagt cctcgcgcct gtccgaccag cggattccgc
     5341 cacgggcgac ctctccgccg cgcaggtgta caccctcggt gcgcggggag tagacgaaga
     5401 tctcgaatct gggcaacggc agaggcaggc ccggaatcct ctgggggtcg agcttgaagg
     5461 agaggtgcgc cttgggatcc ccatccggcg tgctctggaa gtagttcgtc cgcagcgtcg
     5521 ccagagttat gtcgaggaac gagcgcagga tgcggtcctc atccaggctc acgacctcct
     5581 cgagggcttc ttcgatctcc tccttcaacc gctcgctctc cgcctccgct gtgttctggc
     5641 gcgacgggtc gaggcgcgcc tcgaagagat ccacgatgag cctggcgatg tggtggttgt
     5701 tgactagggc gtcttccatg tagttctggg agaacgtcga gcctgcctgg cggaggtatt
     5761 tgcagtaggc cctgaggatg gtgatctcac gccaggtaag tcgggcgcgc agcacgagac
     5821 ggttgaaccc gtcgttctcc acggcaccgc gccaggcgcc ggcgaacgcc tcctggaaga
     5881 tctccttgac ctctccggtc tgtaactcgt cgtcggcctc gtgcacgagg ccgaagtcgt
     5941 agatccagac cggaggcgaa cccgccggct tcacttcgtg cgggcgctcg tcgaccactt
     6001 cgacgcccat gtcctcgagg agcggcagga tctccgagag cgagacctga tcgccgagcc
     6061 tgaacagctt gaacccgagg aagtcttcgg gctcttcgat gggatggtag aggctcatcc
     6121 ccaggtcgtc ctcggatttc agggtctcca tccgctggac gtccgagacg gccgtgcggg
     6181 gcagaaagcc ggcgcggtag ccgggggaga aggcgtcgcg gtacttgcgg aacagctcta
     6241 tgccctgctc ctccccgcac tgttcgatca tggcgtcgta gaggttgtcg gtccaggagc
     6301 gtgtggtctc gacgatgcgg gcttcgatct gctcctcgtc gtattctggg gtgtctcccg
     6361 gcttcgtgta gattatgaaa tggatgcgtg cgagcacgga ctctgagagc ctgacgttga
     6421 actccacgtt cgccccatcg aaggccttga ggagtatgtc ttgcatgcgt tcccggatca
     6481 cggtgttgta gcggtcgcgg ggcacgaaga cgaggcagga gaaaaagcgt ccgtaggtgt
     6541 cgcgcctgac gaagaacctg acgcgctgtc gctcctgcag gtggagtatg cccatcgcta
     6601 tctcgaagag ctcctccttg gagatctgga acaactcgtc gcgcgggtag gtctccagga
     6661 tctcgatcag gtccttctcg ttgtggctcc cctcggggaa accggccctt tccagcacat
     6721 agcgcacctt gcgcctgacg agcgggatgt cgaaggcgct cgcgctgtag gcggagaagg
     6781 tataaagccc gaggaaccgc cgctcccccg tgacctcgcc cgattcgtcg aacttcttga
     6841 tgccgatgta gtccaggtac gagggtctgt gcaccgtcgc ccgggagttc gccttggtca
     6901 ggttcagcag cttgggcgtg cgggcgagcc gtctgacccc tggcgggagc tcggcgaagc
     6961 tgcgggagac cgggccagac tcggtctgcc gcagaatgcc gagtcccgtc ccctgcaccg
     7021 cgcagagagc ttcttcgccg ttctgcgtga tcaggtcgta ctcccggtag ccgaggaatg
     7081 tgaagttgtt gtcgtcgatc cattccagga acgctcgggt ctcggccagg tcctcaggct
     7141 cgaagtcggg aaggttctcc tcgagcccgg aggctatctc cccgaccctc ccgcgcatct
     7201 cgggccagtc ctccacggcc gccttcacgt ctgcgagtgt ctttatgatt gcttgcttca
     7261 agtcttcgag cacgtacggc tcggtctggc ggtccacctc gacgtggatc acggactcgg
     7321 agatagcgtc ttcctcgtct gagtcgggag gtaggacttc gaggagccgg ccttcctcgt
     7381 cgcggcgcac gttcattacg gggtggagga tcatgtgtat ggcgtagccc tggcggttta
     7441 cctccatcct cgtcgagtcg accaggaagg gcatgtcgtc gttcaccatc tctacaacgg
     7501 tatgggtcga ctgccagccg tgttcctcga aatgggggtt gtagacccgg atcttccact
     7561 cccctacctc gcgccgcccc gcgaagctcc aatgggagag cgcggcccca taagcgtcta
     7621 tcggggagag gtcctccagg tccgccgcgt cgacccaccc gtagtattga cgagcgaact
     7681 cctcgacctg cggcgcctga tcctccggca tctgctccct gattcgggct acgaccttgt
     7741 ccagtagctc atccttaacc agcatcggta cgcctccctc ggcggttccc ttcttcccgg
     7801 aatgtccatt atagtccggc gtagtttccc ggtcaggcgc gttaagtgag tctccccgaa
     7861 tctggcaggg catggccgcc cgcgaagcgg accgtccgtg ctaaggtcct attagactca
     7921 ggtaggactg gttgcccgaa tgccagtaca aggtacaaga ccatcatagg ttggtaggag
     7981 gatctcgtga acttcgacct cggtacagac cagcaaggga tcaagaatcg cgcagcaagg
     8041 ttcgccgata gagaagtagc gcctcgcgcg ccggagttgg accgggacga cgtggtccct
     8101 ctcgagacgt tggataaact ggccgacgcc gggttcatgg gcctgtgtgt accgaaagag
     8161 tacggcgggg ccgggatgga cttcctatca tattgtctcc tgatcgagga gatcagccgc
     8221 gcggacgccg gggtcggcgt gacgctcgcg gtccacacca gcgctggcac gctgcccatc
     8281 gtgatgttcg gcaccgaaga gcagaagtcg aggtgggtgc cgccgctggc gcgcgcagag
     8341 agcatcggtt gcttcgccct tacggaaccg gagaccggct ccgacgcctc ggcgatccgg
     8401 acgcgtgccg agcgggtcga ggacggctac cgcatctcgg ggcacaagca gtgggtcacg
     8461 aacggacgcg tcgcagggac catgatcctc ttcgcccgca cggagggcgg agccagagat
     8521 ggcgttacgg ccttcgtagt ctctatggag gctgagggaa tctccttcgg caaacacgct
     8581 cagaagatgg gtgttatctc ggcgaccacg gacgatgtcc tcttcgataa cgtcttcgtc
     8641 ccgaaagaga accggctcgg ggaagagggg agagggcttc gcgtcgcgct cgggacgctg
     8701 gatacgggca ggatgggggt agccgcgcag gccgtcggta tcgccgaggc cgccttccgc
     8761 tacgccacgc aatacgccgc ggggcgcacc acgttcggga agccgatcgc cgagcaccag
     8821 gcgatagcct tcaagctggc cgacatgcag acgaagatca gggccgcccg tctcctcgtc
     8881 tacgaggcag cctggatgaa agaccaggag atgccgcacg gcgaagcggg agcccgcgcc
     8941 aagctctacg cctcgcaggt cgcgaacgag gtagcgtacg aagccctcca ggtcctcggc
     9001 ggccggggat acatgaaaga ccatcccgtg gagcggtact acagggacgc cagggtgacg
     9061 gagatctacg agggcaccag cgagatccaa cgcctcgtca tctcccgcgc catcctgcgc
     9121 gagcacgaag agtccgttgg ggcgccgggc agcgtcgacc agtcgcggcc tgtcgtaggg
     9181 taagcacccg cttatgctac agtcatgcta cagtcttgga gcagggatta gagacgcagg
     9241 aggaacacgc cttggatacc aggaagagcg cggaggaccg ctgggaggaa gcttactcga
     9301 agggcccgga gcgcgacgcc gagttctcga ccatgtcggg cgtgccggtc aaacctcttt
     9361 atactcccga tgacgtcgag ggcgcctacg acgagaagct cggctatccg ggcgagtatc
     9421 cgtacacccg cggtgtgtac ccgaacatgt atcgtggaag gctctggact gttcgacagt
     9481 tcgccggtta cgggacggcc gctgagacca acgcccgctt tcggtacctc ctggatcacg
     9541 ggcagaacgg gctctccgtc gccttcgaca tgccgacgct catgggcctc gactcggact
     9601 cgccgctctc cttaggcgag gtcggcaccg agggcgtcgc cgtagactcc ctggaagaca
     9661 tggagcgtct cttcgagggc atcccgatgg gcgaggtctc gacttctatg accataaacg
     9721 cccccgcgat ggtcctgctg gccatgtacg tagtggccgc cgagaagcag ggtgtcgcgc
     9781 cggaggagct ggagggcacg aaccagaacg acatcctcaa ggagtacatc gcccagaagg
     9841 agtggctctt ccccccagag ccctcgatgc gcgtcttcaa ggatatgctc gtctacgcca
     9901 cagagcacat gcccaagtgg aataccgtct ccataagcgg ctaccacatc cgcgaagcgg
     9961 ggagcacggc ggcccaggag ctcgccttca ccctggccga cggtttcgcc tacatagagg
    10021 ccggtataga agcgggcctc gatgtcgatg atttcgcccc gcgtttcagc ttcttcttca
    10081 actcccacat agatttcttc gaggagatct gcaagttccg cgccgcccgc cgaatctggg
    10141 caaccgtctt gaaagagaag tacggcgcca aagatgagcg ctctctaagg atgcgctttc
    10201 acacccagac cgcgggcgtc tccttgacgg cccaggagcc ttacaacaac gtcgcccgca
    10261 ccgccttcga ggcgcttgca gccatcctgg gcgggacgca gagcctgcac accaactccc
    10321 tggacgaggc cctcgccctc ccgactgagg aggcggtgcg aatcgccgtc cggacccagc
    10381 agatagccgc cctggagacc ggggtggcga acaccataga cccgcttggc ggctcgtact
    10441 tcgtcgaggc gttgaccgac gagctggagc gacaggccta cgactacttc aggcagatag
    10501 acgagatggg gggcatgatc caggccatag agcagggctt cccgatgcgt gagatctccg
    10561 aagcctcagc ccgctaccag cgcgaactcg aagagaacaa gcgctacatg gtcaacgtca
    10621 acatctacga accccaggac gagcaggaga tggaactcca ccgcatcgac cccgaagtct
    10681 cgaaaggtca ggtcgaacgt ctcaaggccc tcaaggagaa ccgagacaac gccagggtcg
    10741 aacggtgcct cgaggagctc aagcaagcgt gccggggcga tgataacacg atgtattcga
    10801 tcctcgaggc cgtgcgtgcc tacgcaacgg tcggagagat cagcgctgcc atgcaggaag
    10861 tcttcggcga ctatcgcgag acgccggaga tctagagtct ctcagcccgc tccagccttc
    10921 agcttccgct ttcagcacgc ttcggcttcg cctcgcttgt cagcaggtcg gcttctcccg
    10981 ctccggcctg ccgcgcacga cgaaccactt ctagcggcag tcgggttcta cgctctcggc
    11041 gaggttacgg aaggactagc tgaagtgctg acgagccgcc gagcacagcg aggcggcggg
    11101 ccgacaagcg aaggcgctag cctgagcgtg ctgactagct gacatgctga tatataatcc
    11161 atttgatact caggtgtcag aatgttgggg ttttctggaa ggagaagcgt tatggaagcg
    11221 agggtggtgg atcttcggat tcttcccacg gaaacgaggg aggttgttcg catcttcagg
    11281 ggctccgtac tgccggtcgc gagacgtcag caggggttca agggagccct cctactgacg
    11341 gatgacgaga caggggtagg gatctctatc tcgctctggg agaccgagga ggacagggag
    11401 gaaggagaag caaacggttt ctaccagcag cagatcagga agttctcgga gctcctgacc
    11461 gagacaccgg ttcgcaagca ctacaacgtg ggcgtcctcc actacgacgc cagcgttctt
    11521 gcgtagccca cgggccaaca gcgtgaacag acgtcccggc gggcttcagt ggtctgctag
    11581 aattcggggg cagaggagtg atttcggctc ttcacgtggg gacctttgag tagaggagga
    11641 ggtctatggc tatccgagag accagtgctg gccacgaggc gctggggctg gatgaggacg
    11701 atctacgcag gatgcactat tacatgcttc tcgcgcggcg ggtcgatgag cggtcctgga
    11761 tcctcaatcg ccagggcaag gccgcgttcg ttatctcctg ccagggacag gaggccgccc
    11821 aggtaggcgc cgcctataac ctgcgccccg gcaacgattt tctgtacccc tactaccgcg
    11881 actcggggat gaccatgctc ctcggccaga cgccccgcga ccagctcctt agcctcctgg
    11941 gtaagaagga agacccgaat agcggcggtc gccagatgcc gggacactac agcagccgga
    12001 ggctgaacat cgtaacatcc tctgctcccg tcgggatcca gtacccgcag gccgcgggca
    12061 cggccctcgc gtacaagatc cgcggcgagg acggagtcgt actggtgtgc ggcggcgagg
    12121 cgtcgaccag cggcggagac tggcacgagg cgatgaactt cgccggaatc cacgacctgc
    12181 ccctggtctt cctcgtcgag aacaacgtct acgccatctc catccacgct tcgttccagg
    12241 tcgcgggctc tatagcgaag cgggccgagg gctacggttt ccccggccac gaggtagacg
    12301 gcaacgacgt gctcgccgtc tacgaggcgg ccagagaggc cttcgaacga gcccgcgcgg
    12361 gcgaggggcc gacgctcatc gaggccaaga cctaccgtat gacggcccac tcctcggacg
    12421 acgacgaccg gaggtatcgc gagcgcgaag agatagagga gtggcgccag aaggacccca
    12481 tcgtacgctt cgagaagtac ctgatggaaa acggcgttct ggacgaagca gagcgggatg
    12541 agatctcgga gcgcataaag gccgaggtcg cagaggcgag caagtacgcc gaggacgccc
    12601 cgttcgcaga tcccgaagac gtactctccg gcgtctacgc tgaggcttag gggtgtccat
    12661 ggcgacgaag aatctgctcc aggccatcca cgagggtctc gccgaggaga tgcgctcgga
    12721 cgagtcggta atggtgctcg gcgaggacgt aggcagggcc ggcggtgttt tcagggttac
    12781 gcaggggttg caggaggagt tcggtgctgc gagggtcatg gacacgccgt tggcggagag
    12841 tctgatcgtt ggctccgcca taggcctctc ggtcaacggg atgcgccccg tggccgagat
    12901 acagttcgcc gatttcatcc cgccggcgtt cgaccagatc gtcaacgaag cggccaagtt
    12961 ccactaccgc tcgaacggcg actggaattg ccccatcacc atccgggcgc cctatggcgt
    13021 cgtgcctggc ggcgccctct accactccca gagcgtcgag gcctggttct gcaatactcc
    13081 gggcctcaag gtagtcgcgc ccacgttccc gggagacgcc aaggccatga tcaagagctc
    13141 catccgagat cccaaccccg tgctcttcct cgaacacaaa cgtacttatc gcctcataaa
    13201 ggaagaggtt cccgacggag atcacgaggt ccccctcggc gaggcccgcg tccaccgcga
    13261 aggtgcggat atcacggtcg tcgcctacgg cctcatgctc tacatggccc tggaggtggc
    13321 cgaaaagctc tccgaagaac acggtataga gaccgaggtc gtagagccta gaaccctgta
    13381 tccgctgaac aaggagaaga tcctggcctc ggcgaagaag acgggcaagg tagtggtgat
    13441 ctccgaggcc aacctgacgg gatcggtctc gggagagata gcggccatcg tggcgcgcga
    13501 agctttcgag tggttggatg gccccgtgat gcgcctgggc gcccccgacg tgccagcggc
    13561 ggctttcgcg aagccgatga tggaacactt cttgccttcg gcggagaaga tagaagaggc
    13621 catgctcgag ctggcgcgct actagaggag ggtgccttgg ctaaaccggt aacgatgccg
    13681 caactcggcg agagcgtgac agagggcacc ataacccgct ggctgaaggc cgaaggcgac
    13741 gaggtagaga tagacgaacc cctcgccgag gttgatacgg acaaggtcaa cgccgagctt
    13801 ccctcgcccg tggcaggcaa gatcgagaag ttcctcgtct ccgaaggctc gacagtcgac
    13861 gtggggaccg agatagccct ggtcgcagcc gagggcgact ccgccgccgt cgacgccccg
    13921 ccacgggaga acgttgccga ccccgccccc acggaagagt tccccgcttc ggacaccgaa
    13981 gcccagcccg tggcgggcga agagagcccg gcagcacgta catcgcgaag cgatggcaac
    14041 ggacgcggcg atgtcgaaga tgccgaaacc ctacgtctca cccgctcctc accggtcgtc
    14101 aggcgtctcg cggaggaaca cggcgtggag atagcggaga tcccgggcac gggcacgggc
    14161 ggacgcgtaa ccaggaaaga catagaaggt tatatcgagg aactggagga gcgcgaagag
    14221 tacgcgccgg gactacagga agagcgggaa gccttcgcgg agcccgagcc ggagatcgaa
    14281 cgcgtccctg tctacgaggg cgaccgcgtc gaggacctga ccggtatcag gagagccata
    14341 gccaatcgca tgtcagcgag caagcgcgaa gctccgcacg cctggaccat ggtcgaggtg
    14401 gacgtgagcg ggctcgtcgc cttgcgcgag cgggagaaga ccgccttcgt cgaacgtgag
    14461 ggcgtgaagc tcacctacct gccttttata gtgaaggctg tcgtcgagag cctcaaagag
    14521 aatcccgtcc tcaactccgt ctgggacggg gaccgcataa tcttgagaaa gcgcataaac
    14581 gtgggtgtgg ccgtcgatct cgaagacgcc ctcatagtgc ccgtcatcac cgacgccgac
    14641 gagttgaacg ttctcgggct cgcccgcaag atcgaggacc tcgtgcggcg cgcccgcgcc
    14701 cgaaatctcg gcactgaaga cgtctcgggc ggcaccttca ccgtcaacaa cccgggctcc
    14761 ctgggaagcg tggtctccac gccaattatc aatcatccgc aggcggcgat cctctctgcg
    14821 gaagcaatcg tcaagcgtcc cgtcgtcata gaagacgcca tcgccgtacg cagcatgatg
    14881 aacctggagg tctccttcga ccaccgcgtc ctcgacggcg gcgccgccct caagttcctg
    14941 aacgccgtca agagccgact ggaggcttgc gggcctgaga gcagcatcgg atagtagtca
    15001 ccgacaatac aaacaccctc acacatccgg cctcgttacc gtaccgggta agcctagaga
    15061 tgaactggct cctggtacac tcaatagcat tctcaagcag gcaggaatga agtagtaaga
    15121 tatgaagtat ctcgtggtta tcgaaccaac tgacaccggc ttttcatctt actccccgga
    15181 cttacctggg tgcgtatcga ccggagcgtc tcgtgaagaa gtcgagcaga acatgcagga
    15241 ggccatagaa ctccacctcg aggggctgcg cgaggaaggc tattctgttc ccgagccgag
    15301 cacctcttcg tcgtatgctg aggtgataca gcgtcttgca taagggttca accctaatgc
    15361 acgccgagct ttcagcgata agcaagaaca aaaagctgaa ggctgaaagc tgagagctga
    15421 gggcgcgggt tgcaggagct caatccttct actacccgct gtaacggacc cgttgaaaag
    15481 actatccctg cggcacgagc gcgagcgccc gtacggggct gccggtaccg ccggtcagct
    15541 tcagcggcgc cacgaacagt agtgctccgg tttgcggcag ggcgccaagg tttgcgagct
    15601 gcgtaacccc gtactttccc gctcccatga gatagaagtg gaccgggaaa gaaggatcga
    15661 actcggccgc gccacccgcg tcgatgccga ccgtctctac cccgacgccg acgagggggc
    15721 tctcc
//
LOCUS       DATMVF010000285        43783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_686828, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000285 DATMVF010000000
VERSION     DATMVF010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 43783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43783
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686828"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..414)
                     /locus_tag="VN178_14855"
     CDS             complement(220..414)
                     /locus_tag="VN178_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82297.1"
                     /translation="MQAVEQRSTSKSEAARLFGISLSSVKRYTKLANREESLTPMIFE
                     LLRQRDGRPLHISSLSPLEM"
     gene            819..1202
                     /locus_tag="VN178_14860"
     CDS             819..1202
                     /locus_tag="VN178_14860"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS82298.1"
                     /translation="MEINKDLIAASSTPIVLAILAEGDSYGYAILKRVRELSGGRMEW
                     TDGMLYPVLHRLERLGHVEARWEIAESGRHRKYYRISPGGRAQLAEERRQWQAVDATL
                     RGIWRAHSLLVPDGHPAVAAPRGAE"
     gene            1202..2575
                     /locus_tag="VN178_14865"
     CDS             1202..2575
                     /locus_tag="VN178_14865"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HWS82299.1"
                     /translation="MPAPDDAVSVEEQIARWRSYLGRRQAIHPVDVAELEDHLREQIV
                     VLAEAGLTADEAFLVAVKRMGKLDALSREFAREHSDRLWKQLVVSPSDTGGQWPAARA
                     DAIVVFCLAVAAAVVIKVPTMLFGIHLDQDAGFYARNLSLFVLPLLTGYFVWKRRLDT
                     TTIRWLALAFVAAGVFANVYPFATEGYTETLTALHLPIALWLVVGIAYVGGRWSEVAG
                     RMDFIRFSGELFIYYVLIALGGAVLMASMRIIFQSIGIDVEPFFESWLLPCGAVGAVV
                     VASWLVEAKQSVIENMAPVLTRLFTPFFAAVLVAFLVTLLWTGRGVDIGRNALIAFDL
                     LLVVVLGLLLYSVSARDPRSPPGAFDVMQVVLVVSALLADAVALWAIAGRITEFGFSP
                     NRVAALGENVILLINLAWSAVLYLRFLRGRESFSALERWQTNYLPIYAAWAAIVVIAF
                     PPVFGYI"
     gene            3037..3585
                     /locus_tag="VN178_14870"
     CDS             3037..3585
                     /locus_tag="VN178_14870"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HWS82300.1"
                     /translation="MEEGRQLSAPTLLKAALAYAGRGVPVFPCERGAKRPLTRNGHWD
                     ATTDRGVIEQWWRRQPSANIGLPTGEKSGIVVLDVDVDDGGLGSLAKLEREGAPLPKT
                     ARTRTGGGGLHLFFRYPRGTEIRNTAGLLGPGLDVRGEGGYVVVPPSRTQGPYEWVDR
                     SPLAEASWLIDRLRELDGARLF"
     gene            complement(3881..4435)
                     /locus_tag="VN178_14875"
     CDS             complement(3881..4435)
                     /locus_tag="VN178_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWS82301.1"
                     /translation="MRKVVALELVSVDGVMERPEEWAFAYADDEMNEANAAGMAASDA
                     MLLGRMTYEGLAAYWPNQPAGTPMVDYINSVPKFVVSTTLEEPLGWNNSTLVEGDVAE
                     GVAGLKRRAGKDITIIGSGALVRSLLEEGLLDELRLLVHPVILGGGKRLFEDRGVPVG
                     MTLVDSKTFGTGVVSLAYRPSVEG"
     gene            complement(4603..5136)
                     /locus_tag="VN178_14880"
     CDS             complement(4603..5136)
                     /locus_tag="VN178_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82302.1"
                     /translation="MLFGNLLALTVLIFGVVALYAYLANGGQRTLALGAMMSSIVGIA
                     LMLSRLGVFAYAVPALSRSFLDGNPESIRIVDSIFAGPLETVESVSILLYSAGFVLFG
                     IAIWRSSVLPGWTGVLIAVHAPLVSGPFPLVGSVAGALLALAGGGWIAFSVLRDTPGQ
                     AESRATLRVGRGNFREI"
     gene            complement(5325..5825)
                     /locus_tag="VN178_14885"
     CDS             complement(5325..5825)
                     /locus_tag="VN178_14885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS82303.1"
                     /translation="MNGTRARRGRGPESAIGVQIPAGRAEAQTNGKGGEPMIKDVPLV
                     GIFVNDQDAALDFYAGKLGLVKVQDESYGESARWITVSPEGTGTRIVLKKAEHDHERA
                     LVGRSDGAPILTLGTDDVRAAYEELRGRGVRFLGEPYRYPWGFAAILLDQDGSPLLLQ
                     QESEER"
     gene            complement(5822..6832)
                     /locus_tag="VN178_14890"
     CDS             complement(5822..6832)
                     /locus_tag="VN178_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82304.1"
                     /translation="MVERDEDLLPRSMKAQFVRAPVRRSLAGRAWASSRPLTFVGAAM
                     LLVLGVAVVGLLLDERVITGAPAWLKPMKFAVSISIYCFTLLWMLTFVRGRPRLVALI
                     SWVSAIALFVEMVLIAGAAALGTTSHFNVSTPTHAAVWYTMSAFVALIWAANLLAAAL
                     LLVQRMPDPAFAWALRLGILVSLVGMAVAFPMTQETPAQERAADKAGIEEWPIQGAHS
                     VGVKDGGPGLPVTGWSTTGGDLRVPHFVGLHGLQALPLFGYLLVLFAPAWLRPGHRVT
                     LVWTAGLSYLGLVGVLTWQALRGQPVISPDPATLGTLCAVLLIAGMTASAVVLRARSG
                     RR"
     gene            6983..7996
                     /locus_tag="VN178_14895"
     CDS             6983..7996
                     /locus_tag="VN178_14895"
                     /inference="COORDINATES: protein motif:HMM:NF039924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5937 family protein"
                     /protein_id="HWS82305.1"
                     /translation="MITIPLSTEDLTKVRLAPSPLWETVTSFAVLAYRGRDTVHAPWA
                     ARARRVLPGTDLSALVNAMCLQRACPDFLTPPPDASVASFDEELERLRATPAEVVYEE
                     VESLVLVEKDLFGLSPEKERMLGIYLKDPEGSLRRLVDTLNRYHELAVSPYWPRMREH
                     LEGDTIRRGQALALGGVEALLSGLSPKVNYSGGAIELAKAYERIVDPKGRGITLVPCV
                     FVWPKVDVLVQPGYQPTLAYPPRGAANLWTASSPRESSGTALGAALGVSRASVLKCLL
                     PAPTTTMELSQQLRLAPAAVSAHLSRLKVAGLVEPHRSGRKVYYRLSSAGESLLEIFG
                     ETG"
     gene            8336..8764
                     /locus_tag="VN178_14900"
     CDS             8336..8764
                     /locus_tag="VN178_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS82306.1"
                     /translation="MSIFPTRILLATDGSEEARLAATTAADLNKSTDSELHVVYVGEL
                     RPTFLAQTEQEPAEVEREAQRLLDEQVRRVEEAGGTVEEAHLRLGRADEEIVDLADSL
                     GIGLIVMGSRGHGRIRRALMGSVSDSVVRHAHCPVTIVRE"
     gene            8862..9332
                     /locus_tag="VN178_14905"
     CDS             8862..9332
                     /locus_tag="VN178_14905"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWS82307.1"
                     /translation="MPDNEPDNPGIKVPPPLIYLLPLLLGLILHTKAHLPILPNSVVR
                     MVGWPLIGCGVLIGSWWRKTMRDADAPVRTDRPVPRLTTAGPFGYSRNPAYLSLAMIY
                     AGTAALRNSLWAMLFLPLVLYVIQREVIGREERYLERTFGEEYLAYKARVRRWV"
     gene            9503..9706
                     /locus_tag="VN178_14910"
     CDS             9503..9706
                     /locus_tag="VN178_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWS82308.1"
                     /translation="MAQGTVKWFSDEKGYGFISPDDGGEDLFVHHTGIAGEGFKTLDE
                     GAKVTYEATQGRKGMQAENVTPA"
     gene            complement(9832..10785)
                     /gene="argF"
                     /locus_tag="VN178_14915"
     CDS             complement(9832..10785)
                     /gene="argF"
                     /locus_tag="VN178_14915"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWS82309.1"
                     /translation="MVIPDLQNAPPPSPLAGRDCLTLGEFTPGEMSLILDEAVRIKAL
                     QKSHIPYRPLRGHTLAMVFQKPSNRTRVSFEVGMYQLGGHALHLSPEEIQIGKRETPS
                     DTGRVLARYIDAIMVRTFDHGDLEELAGAADVPVVNGLSDTHHPCQALADLMTVREEF
                     GTVEGTKISYVGDGNNVAHSLAIACALSGAELTIAHPEGHGPEREIVELAGSLGAAPH
                     LTDDPDEAVRDARVVYTDVWASMGQEDEAEERKEKFAPYQVDEELMNLAAEDAIFLHC
                     LPAHRDQEVTAGVIDGPRSRVFDQAENRLHAQKALLYLLMG"
     gene            complement(10788..11981)
                     /locus_tag="VN178_14920"
     CDS             complement(10788..11981)
                     /locus_tag="VN178_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWS82310.1"
                     /translation="MQAVMETYKRLGIAPTGGEGSWLFDSEGNRYLDFIGGIATNSLG
                     HGHPALVGAIQEQAGKLIHCSNLYEIPLQAEVAEMLCGATDFDRVFFCNSGAESVEAA
                     IKLARKYARDTYGPNKHEILTFSKSFHGRTYGGLTATAQPALQDGFGPMLPGMVHTPF
                     GDLEAAEALVGPHTAAVLVEPIQGEGGVNEPPEGFLRGLRELCDEYGALLIFDEVQTG
                     AGRTGYLYAYQGTDVVPDVLTSAKGLGGGLPVGAMMVKEEYAALGPGNHGSTFGGNPL
                     AMAAVKAVLGVVNDPAFLEEVRFKGTVLKNALQKLAEQIPGAEVRGEGLLLGFDFGDA
                     GLAAEVFEHCLDHGVLVNLVGGTTIRMAPPLTVSRTEVRAALDTFRGGIYQTVEETVP
                     EAAVA"
     gene            complement(11969..12736)
                     /gene="argB"
                     /locus_tag="VN178_14925"
     CDS             complement(11969..12736)
                     /gene="argB"
                     /locus_tag="VN178_14925"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWS82311.1"
                     /translation="MRPVVIKIGGGILAGGALEDLPDLLSAGHRVVIVHGGGLQLTRV
                     LDALGIATRFHEGLRVTDEETIEVAENVFAGGVNKGLVRGLGDLGVQAAGISGTDGPT
                     LLVEPVPDLGRVGRVVRVGPQLIEALWAGGFVPVVAPLGLGPEGAYNVNADSAAAALA
                     VGLGAGHLFLLTDVDGLLEEGETVETLDPDGCEAYVDSGLAAGGMVPKLRAAASAARG
                     RVEARILNGNRKGALGAAVAGERVGTLISEGVAACKQ"
     gene            complement(12733..13911)
                     /gene="argJ"
                     /locus_tag="VN178_14930"
     CDS             complement(12733..13911)
                     /gene="argJ"
                     /locus_tag="VN178_14930"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWS82312.1"
                     /translation="MTAASGFVAAGVGCGVRDVGRRDLGLLFSELPCETAAVFTRNAV
                     WGAPLVVTREAVESGGVRAVVANSGNANAATGERGIEDARAMQALGAEALGLEAGEVA
                     VASTGVIGVRLPMDRISSGISAASSELGEGDEGFAGSILTTDTRAKEAVARVEIGGRT
                     VTVGGTAKGSGMIHPNMGTMLAFLTTDAAVEKSCLNETLSRVTDRTFNRVTVDGDTSP
                     SDMALLMANGAAGNEPLTLDSPDYPAFAEAVEGVARTLAREIARDGEGATRLVEVAVE
                     GAASEESAAALAKSVVGSNLVKAAVFGEDANWGRVLTAMGYSGEVFDPEGVELWFGPI
                     KVFSGGEPVPHEEAEANATLAAGEVKITVRLSEGDASAAAWGCDLSYEYVRINGSYRT
                     "
     gene            14049..15059
                     /gene="argC"
                     /locus_tag="VN178_14935"
     CDS             14049..15059
                     /gene="argC"
                     /locus_tag="VN178_14935"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWS82313.1"
                     /translation="MGLRVGVYGGSGFAGIELVRLLAGHPEVGAMSVASRGYVGLGIG
                     EVYPQLAVDGTYVAPEEIEASDLDVAFVAYGHGESAGAVKELLGSGTRLVIDLSADFR
                     LPQEELYVEWYGEHPAPELLGEAHYGLPEVFGATEGRLVANPGCYPTAAILALAPVVR
                     RMGAGIRSVTINALSGVSGAGAKPSVKTHFVSVNESAGAYGAPRHRHTPEIETMLRRV
                     GETPEVTFVPHLIPITRGELETIVVDVGEVPEAEEVLGWYEEDYEGWAFVEAREEYPR
                     VAHVANTNRCRLSAAVDGRAGKLLLFSAVDNLLKGASGAALQNMNLALGYEEYLGLEH
                     LT"
     gene            15070..15549
                     /locus_tag="VN178_14940"
     CDS             15070..15549
                     /locus_tag="VN178_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HWS82314.1"
                     /translation="MGATMSPVDKTTRQDLILRLISERDLGTQQDVVGALADEGVEVA
                     QATVSRDLAELGVLKVGNRYLALPHDPGSAGIEVLPSFVLGLMPAQNQVVIQTRDGTA
                     GAVSSVLDRVKGLKIVGTIAGQDTVLAIAPDAAAATEVANLIADVCRAKTRPAGNVE"
     gene            15631..16857
                     /locus_tag="VN178_14945"
     CDS             15631..16857
                     /locus_tag="VN178_14945"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWS82315.1"
                     /translation="MLEGKKVVLAYSGGLDTSVCVKWLEQQGAEPYALYLDLGQGEAS
                     EDVKAKALKIGASDAFVRDAKAEFAEEYVAPAIKANALYGGKYPLFTALGRPLIARKL
                     VEAAREVGATHIAHGSTGKGNDQVRFDVTTASIAPDLTVVAPVRDWNMSRPEEMAYAE
                     EHGIPVPTTKESPYSVDENLWGRSIEAGPLEDPDHEPTEDVFALTTSPENAPDEPQYV
                     ELGFQEGLPTTLDGEELPLVDLISELGSVAGANGVGRIDMLEDRLVGIKSREIYEAPA
                     ALVIIAAHRELETLTLTRDVLRFKTAVEQRYAELTYEGLWFTPLKSALDAFIAETQKT
                     VTGAVRLKLYKGNSTVVGRTAPNALYSEDLATYDPNSTFDEAAAAGFIALWGLSARQW
                     ASVNGSTEYNQISPQH"
     gene            17151..17978
                     /gene="arsM"
                     /locus_tag="VN178_14950"
     CDS             17151..17978
                     /gene="arsM"
                     /locus_tag="VN178_14950"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HWS82316.1"
                     /translation="MEEDLKQRVRDRYAGAARSVLGEAEDSASCCGVAESGERAAFEV
                     DMVCGSYSEAERSELPQLAADASLGCGNPVALATLSPGEVVLDLGSGGGIDVLLSARR
                     VAPGGRVYGLDMTDEMLELARRNQAEAGVENAEFLKGEIEDVPLPDGHVDVVISNCVI
                     NLSTEKQRVISEAFRVLKPGGRFAVSDVVFLGEKNKLPPGVARSVELWSGCVSGALEK
                     GEYEALLSEAGFEEVAVEVTHTYPPEQIAGLSGEDAEALREVPAASAFIRAGKPEDL"
     gene            17981..18397
                     /locus_tag="VN178_14955"
     CDS             17981..18397
                     /locus_tag="VN178_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWS82317.1"
                     /translation="MARQRLPVLQQSSVAPGTIDVGGEERAGRLVAFGRALSDPIRVQ
                     MLGMLAGGRGCCELPDCGVPAKDQDAGICVCEFERFFDAGQSKISYHLGKLKAAGLVH
                     EERRGKWSFYSLDREAAGRLLAETAANLDGDPRDEG"
     gene            18387..18812
                     /locus_tag="VN178_14960"
     CDS             18387..18812
                     /locus_tag="VN178_14960"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006637136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWS82318.1"
                     /translation="MKADRRAEDRTRVLFLCTHNSARSQMAEGLLRGMAGDRFEVFSA
                     GTEATRVRPEAISVMAEIGVDISAQESESLDRYLGETLDLVVTVCADANEACPLFPGA
                     RERLHWSFPDPSKAAGGREERLRDEIRARIEGELAKPGM"
     gene            18873..19385
                     /locus_tag="VN178_14965"
     CDS             18873..19385
                     /locus_tag="VN178_14965"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWS82319.1"
                     /translation="MPAPPTHDPSETDLTMMRRALLVAEEAANKGEVPVGAVVARGRE
                     ILAVAANEREATGDPTAHAELLAIRRASATTGGWRLTGCTLYATLEPCPMCAGAAHAS
                     RLSRLVYAAPDPKAGYAGTLHNTPTDSRLNHTLIVESGLLEESAAALLRDFFRYRRRR
                     PGEASESSLA"
     gene            19726..20202
                     /gene="nrdR"
                     /locus_tag="VN178_14970"
     CDS             19726..20202
                     /gene="nrdR"
                     /locus_tag="VN178_14970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWS82320.1"
                     /translation="MQCPYCDFEDTKVIDSRLSETKDAVRRRRECLSRSCGKRFTTYE
                     RREPLRLMVIKRNGGKEPFDREKLRSGMAKACAKQQVTDEQVELIVDEIEAELRERRR
                     HEVTSRRLGDMVLMRLRRMDMVAYLRFASVYRQYTDVDQFRTELVRLAGSGMGNGR"
     gene            20289..22595
                     /locus_tag="VN178_14975"
     CDS             20289..22595
                     /locus_tag="VN178_14975"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWS82321.1"
                     /translation="MEVDRKRLAEIDLTENARAVLGKRYLKKNEKGEPVEEPIEMFLR
                     VASNIAEGEFRFKEGEEAQGLYEESRERFLQIMLSRKFMPNSPTLMNAGRELQQLSAC
                     FVLPVEDSIDGIYDTLKHQAIIHKSGGGTGFGFSRLRPKNDLVKSTMGVSSGPVSFMA
                     IYDASTDKIKQGGTRRGANMGILRVDHPDIEEFIDCKRDNDQINNFNISVAITDEFMQ
                     AVEADTDFELKNPRDGEVIKTVRARDLFKKIVEGAWLNGEPGVVFIDKINDDNPTPQF
                     PIESTNPCSEAHLPPYDSCNLGSINLERFYREDTGDVDWDELRETVHTAIRFLDNVIE
                     QNNYPLPEIDEMSRGNRRIGLGVMGFADLLIKLGITYDSEEGLAFAERIMKFVDDEAW
                     EESRTLAEERGVMPNYEGSRHEARGDRVRNATVTTIAPTGTISMIAGCSGGIEPLFAV
                     AFMRRQADMEMPDVNPEFVKLARERGFYSEELMKKVAQHGSVRDVEEVPDDVRSVWVT
                     SHDISSEWHVRMQAAFQKYTSMGISKTINLPNEATPEDVEDAYRQAYALNCKGIAVYR
                     DGSRDAQVLSTGKTGGEKPTMTPASPEAPTSPPVTPPAGAEAQPQNFSSLAEARNGRP
                     RTLTGVTKKIRTGHGSMYVTMNDDEFGPRECFVILGKPGGTAAAFSDALGRMITMALT
                     HGATPAEVVHQLRGIQDGHPAGVGPNAVLSVPDGVARAMSENYLIEDTLPDTRELPII
                     GACPDCGSGLTRQEGCVVCHSCGYSKCN"
     gene            complement(22644..23582)
                     /locus_tag="VN178_14980"
     CDS             complement(22644..23582)
                     /locus_tag="VN178_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWS82322.1"
                     /translation="MTKGNVTNGVTARGYAELSIAAAVTTIGLKLGAYFLTGSVGLFS
                     DAAESVVNLVAAVAALWALSLAVRPPDEEHAFGHNKAEYFSSGLESALIIIAAAWIGV
                     TAWDRLMDPQPLQNVGLGLSVTLVAAALNGIVALVLLRAGRRLRSITLRADAQHLLTD
                     VWTSAGVVLGVVMVQLTGWLVLDPLIGLVVAANIVRTGFRLLRDTAQGLLDRALPQED
                     QEVISRVLSHYESQGIRFHAVRTRASGQRRFVSMHVLVPGQWTVQQGHDLSEKIEGEV
                     AEALPQSTFFIHIEPSEDPASFEDQSLDRERRDTEA"
     gene            24160..24249
                     /locus_tag="VN178_14985"
     tRNA            24160..24249
                     /locus_tag="VN178_14985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24194..24196,aa:Ser,seq:cga)
     gene            24569..25720
                     /locus_tag="VN178_14990"
     CDS             24569..25720
                     /locus_tag="VN178_14990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HWS82323.1"
                     /translation="MITAGIWVLGSSLWMYIDYMGATLERISTRSMAFHVDFDSFWRS
                     ARAMLEGQSIYDTGVDLVNLNPPLWTVLISPLGLLEPITAYRVFVLISLLIVVGYLAW
                     TVEELRLRPGWTVVGVLMLLLSSPLLATLALGQVYPVLALGLVAAWVADRRDEQEISG
                     AALGLVVALKPSLLPVLLWPLARRRWRALLAALASGAVATLIGVAVLGPGETLDYIEV
                     LREQPVSAYWDNASLPSAAARLFTENPYAQNVATLPWMVVVGFALGIMAIFITAMRVR
                     HGPEVGLWVMVAASLLASPIAWHNYLVLLGPGILLLLARGRAATAFLLLALQSIPAQW
                     PLIWNDRSTVAASLAMTLYLYILIAHWLALLAATREPAGMIRPAAEEPG"
     gene            25797..26693
                     /locus_tag="VN178_14995"
     CDS             25797..26693
                     /locus_tag="VN178_14995"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWS82324.1"
                     /translation="MAETLMQRVVEALPFLDDIADDVQPKVQEAVAAGGTTARDVLDG
                     VWLEAPLHPVLTDVPVGAWTAALVFDGLDIATGKEPVRHAADASLVFGTLGALGAAVT
                     GLSDWRYLSGGSRRMGVAHGLLNAIGLILSIVSIILRASGRRNAGRLTFLTGYSISGM
                     AAHLGGELSYHYGLRVDRNVFQGTGPDEFVPVLGESELAQDDLRRVEVEGVGVLLSRT
                     SAGEVCAISATCNHFSGPLEEGEREGDTVVCPLHNSRFELCSGEVIDGPAIFPQSRYE
                     TRVRDGKIEVKAAAENIQKKVR"
     gene            26755..27462
                     /locus_tag="VN178_15000"
     CDS             26755..27462
                     /locus_tag="VN178_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HWS82325.1"
                     /translation="MLSAFIFGLVASSALVIGAVVGAYWTPPRRLLAATLAFASGALI
                     TALAFDLFLTSFERGGAWLSSIGLLLGAAAFVGADEALDRYIAGAGSGVSGFALLAAV
                     TLDGIPENLALGVSLLETPTGGTLTLLVAIFLSNLPESLGGAVGMRQQGRSRLFAIGV
                     WSATALILAVAVVLGNVSLSSAGEGTLAVLLSFAGGAVLASLADTLMPDAFREGGKWV
                     AFATAAGFLLSFLIAEV"
     gene            complement(27740..28354)
                     /locus_tag="VN178_15005"
     CDS             complement(27740..28354)
                     /locus_tag="VN178_15005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS82326.1"
                     /translation="MKLLYPTLGALVILMVFTFLIGRQNRDLLRVRAYRDFFLRLIPP
                     LSTGVFIAGLPFVMNLATVENYGPVLLVYLGGAAVLTALMTRAVTPDERRAGTAFRKG
                     EYEKAANLYDEICARRPLPRYYSALGASLDASGNPGAALEATDQAIKLDPKLGIAHYN
                     RASALAALGEMARARGDLQKVFQVDSSRSLRRAAAEALETLERS"
     gene            complement(28351..28953)
                     /locus_tag="VN178_15010"
     CDS             complement(28351..28953)
                     /locus_tag="VN178_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS82327.1"
                     /translation="MGPGAITIVLIFLLLFVSWQTIYAANRDLARARAWGDLARKALP
                     FLALLLLVLSLFLVQSFGQGRMLLAWMAVSAVAIIGASFLSTSSLERNANRAFRAGDY
                     ARAVDLYEKLAAEKPLARNKAFLGAALGASERYEESVDASTKAIEEDPEYGLAYYNRA
                     LVLQRMGKKSRAVKDLEKAQETDLPRRFRSASKRMLEELS"
     gene            complement(28963..29547)
                     /locus_tag="VN178_15015"
     CDS             complement(28963..29547)
                     /locus_tag="VN178_15015"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HWS82328.1"
                     /translation="MDTLQHVLKEWAVAVRALERGETAIVVRKGGIREKAFAVPRTRF
                     LLLPGYEHQRPELLKEEYRPLMDGIPDLTADGPLRFTSFAEVEGAYEISDAAELSALD
                     AHHMWTPEYAESRFKWRPKKPLTVLVLRAYLLPEKVELPYRDEYGGCKSWIGLQEPVS
                     VEGARAALSDEDFDRLIAPALEVLESLEPAPVGS"
     gene            29629..31020
                     /locus_tag="VN178_15020"
     CDS             29629..31020
                     /locus_tag="VN178_15020"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWS82329.1"
                     /translation="MTQQEMPHRVERDSMGEVQVPREALFGAQTRRALDNFPVSDLRF
                     QRRFIQALGAIKLESANVNNELGLLDDELKDAIVEAAQEVVEGRLDNQFVLDIFQTGS
                     GTSTNMNANEVISNRAIQILEGELGSKSPAHPNDHVNQGQSSNDVIPTAIHLSALVAI
                     KEDLLPGLHKLQEALDVKAREFDSVVKTGRTHLQDATPIRLGQEFRGYAGQIERGIQR
                     VDNAVEDLAEVALGGTAVGTGVNTHPDFAPRVCERLSARFGIEVRETENHFQAQSAMD
                     AAVYASGALKTVAVSLLKISNDIRWLGAGPRANLAEIALPEVQPGSSIMPGKVNPVIA
                     ESAAMVAAQVIGNDATIAIAGQSGNFELNVMLPVIAYNLIQSTEILGSASANLADQCV
                     SGLEATERGPALVEQGLMLATALAPVVGYDKAAEISKEAYKTGKTIREVARESTDLSE
                     EELDRLLDARKMT"
     gene            31155..31409
                     /locus_tag="VN178_15025"
     CDS             31155..31409
                     /locus_tag="VN178_15025"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWS82330.1"
                     /translation="MERTQIYLTKEERRTLAVIAERTGRTQSELIREAVDGFLDRYGE
                     RNRLQLLRGARGMWKDRDDLPDFGALRKELDERGEAAEEG"
     gene            31413..31796
                     /locus_tag="VN178_15030"
     CDS             31413..31796
                     /locus_tag="VN178_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWS82331.1"
                     /translation="MAERLLIDTDVMIEYLRGRSEAVEYLEGLTSDLCISVISVAELF
                     AGVRGDEEERSLKQLLLAFVALPVTEKTARLGGLYRRDYGGSHGTGLADALIAATSEE
                     NGADLVTFNRRHFPMVSRITVPYER"
     gene            31884..33428
                     /locus_tag="VN178_15035"
     CDS             31884..33428
                     /locus_tag="VN178_15035"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HWS82332.1"
                     /translation="MISTPSTPVDVPEPGSAEELERFAALQAKLEPLARRVISDPRAP
                     QTVVVVPSLTLDVEELAKIPGVHHYEERLLCMLMLLRLPRTHLVYVTSQNIATAIVDY
                     FLHLLPGIPLRHARSRLTLLSCHDASDVPLTQKILDRPRLVERIRSAIADTDAAHMTC
                     FNSTALERSLAVRLGIPLYGNDPQLNYLGTKSGSREAFREAGVPLPEGFEHLRDGEDV
                     IEAVVELKRRKPGLRRAAIKLDEGFSGEGNAVFAYEGAPEGRDLARWVREELPGRIKF
                     EAAGETWGRYERKFEEMGGIVECWLEGEEVRSPSVQCRINPLGQANVVSTHDQVLGGP
                     SGQIFLGSTFPADAEYSRDIKEAGDRVGKVLKDRGALGRFAIDFISVRRGEGWEHAAI
                     EINLRKGGTTHPYLILRFLTDGTYDANDGLYCTPTAKPCYYYASDNLQSPVYKGLTPD
                     DLVDIAVDNGLHFDGASQQGVVFHLIGALSQYGKVGTVCIGDSHENALKFYRDTVAVL
                     DREAQQ"
     gene            complement(33479..33895)
                     /locus_tag="VN178_15040"
     CDS             complement(33479..33895)
                     /locus_tag="VN178_15040"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HWS82333.1"
                     /translation="MFEQLQEHWREFRESKPGQRFKDRYRRRRQDEQGHILWRIFLIT
                     LGAVIALGSLILAPLPGPGWGTVFIGLMILAGELLPAARFLDWLEVWLRKLGGYVYEV
                     WRASVWGKITVILVSALCVAAVAYVVYHLLFRVWLF"
     gene            34029..34592
                     /gene="msrA"
                     /locus_tag="VN178_15045"
     CDS             34029..34592
                     /gene="msrA"
                     /locus_tag="VN178_15045"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWS82334.1"
                     /translation="MALDDRMNGSAREVATLGGGCFWCLEAVYQELRGVEKVESGYSG
                     GHVPNPTYRAVCSETTGHAEVVQVTFDPSVVSYKDILEVYFTIHDPTTLNRQGADVGT
                     QYRSVIFYHDDEQRRVAEGVISDLESEGIWSAPIVTEVEPFDEFYVAEDYHQNYFRNN
                     GFQPYCQVIIAPKVARFRKEHLDRLKA"
     gene            34618..36495
                     /gene="ggt"
                     /locus_tag="VN178_15050"
     CDS             34618..36495
                     /gene="ggt"
                     /locus_tag="VN178_15050"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWS82335.1"
                     /translation="MKRAILAILFAVVLCSCSTGSTASDGAAGEDAGGPATTVEQTVR
                     ETTAAPDESKSEETTERGSSATTAEPEVWEGPASRNTAVRPAVGTNGMVSSAHPLATR
                     AGLEIMEDGGNAFDAAVAVAASLGVVEPMMSGVGGYGAIVVYSAEERRTRFLEVGSRT
                     PASLDPSVFRPPTPGYAENRCGARAIATPGNLNAWETMWEEYGEAEWPRLFRPAIGYA
                     DRGFPLDRETAAWLGSEYAAFPAHAQEIYGPEGIPLAAGESLVQDDLADSLRLISEEG
                     SEAVYEGRLGSAMAAAAQEGGGFLSEEDLRRNRAEWRETIGLDYRDHRVVTAAPPSTA
                     WAALVRLGVMGRYDAVPAGHNTTAYVHAATEISKRVSKATRRFAADPEKAPTPLDRLL
                     SDEYLEREAASVDPNAVAPYDPPAQFDTAATCSPTGYRPTGYPETQRHTTHFVVADRE
                     GNVVSATQTLGNVFGSKVMPEGTGIWLNDALAWSRFEPVGNVFDVYPGRQPLYALCPT
                     IIMRDGKPRIALGTPGGRTIQQTTPQMIANMLDFDMNVQQSISAPRFSHIVPDLLVIE
                     EGIPQYVSDDLSALGHDLIPAEYPLGNAHALTIEYTPGGETTRFTGAADPRGGGSAAG
                     Y"
     gene            complement(36619..36975)
                     /locus_tag="VN178_15055"
     CDS             complement(36619..36975)
                     /locus_tag="VN178_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82336.1"
                     /translation="MISNEMPQPPYVLLFEEVVPDARPLTILVDDRGAVPLFDSEVKA
                     GAFVASTDFGAALTPVEVSGAGLVRALESVGYEVDYVAINPPPAGEGPMKVRMGGLEE
                     LIEALQTSQEDDLFGL"
     gene            complement(36979..37299)
                     /locus_tag="VN178_15060"
     CDS             complement(36979..37299)
                     /locus_tag="VN178_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS82337.1"
                     /translation="MEIRSIPDAVAFSEEKMKKNALFDSPHLFYDAYCLLPGQSQKVH
                     AHEGSDKVYYVLRGTGRFRVGEEEQDLGEGNAVIARAGDPHGVRNETRENLVILVTMA
                     PRPS"
     gene            complement(37382..38551)
                     /locus_tag="VN178_15065"
     CDS             complement(37382..38551)
                     /locus_tag="VN178_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWS82338.1"
                     /translation="MNSDVGQNLLVIGAPEHDADAYHLSGFLAPDAVICLRVAGKRYL
                     AVSSLEYGRAEKQAPVDELLSYEELGIIDLARELKSGARAYGAAVANLLDEIGASNSP
                     VVVPAHFGIVYADELRARGITLTPDGKLFGGLRRAKTEEEISYIEGTQRAIEAACAHC
                     VGILEEAEVGEDDVLDWRGETLTSELLRSEIDVELLRRGCAADGTIVAGGPQAADPHE
                     RGHGPLKAGESIIVDIFPVDLSTRYYSDMTRTFVKGEPNEGLQEMYDAVLESQEAALS
                     MIRSGVNGKDVHRKVAEVLHEAGYKTNVHDQEEGKPLTEGFFHGTGHGVGLELHEAPR
                     VSLADEELIPGDVVSVEPGVYDPNVGGVRIEDLVVVTEDGCRNLTKFPKDFRIRQ"
     gene            38648..38887
                     /locus_tag="VN178_15070"
     CDS             38648..38887
                     /locus_tag="VN178_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWS82339.1"
                     /translation="MQRQNLHSRVEDALDKVRPALRADGGDARIVACDEKNGSVSIQM
                     LGACKGCPLSQLDFVYAIESLIRREVPEVREILAV"
     gene            complement(39153..39671)
                     /locus_tag="VN178_15075"
     CDS             complement(39153..39671)
                     /locus_tag="VN178_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82340.1"
                     /translation="MFKLYLTSILVAEVVAVVAVASLAGLSLWFLVPIITLTLVAAFI
                     VRRRLGGPAYGIRRLSPQAIAGYRARDPYTNSPEALSHAIVERAREIQHALEESPSDE
                     VRVEMCALGYRACANDMITLTHLTNEALPDASFLKRVRLRRSRKKAIDALSEARKALP
                     PGALRTAPQERQ"
     gene            39773..40354
                     /locus_tag="VN178_15080"
     CDS             39773..40354
                     /locus_tag="VN178_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82341.1"
                     /translation="MQKDPSPEFDLRRPFASAVAVIRTVLFSPRSFYVNFKVEGPLKE
                     PAAFVLLVGAVCGILGAAVALSTNLLFGDLDAGDLRAAALDALLFALLSPVGVGVVAG
                     IYLLSIRTFVGKVSGFREVYRMAAYAAAALVLAWIPVLGAFAFSYALIVLLGIAIQSV
                     YGTTFLTTFVTTVVGFVPVASVVIWLLIAGVAS"
     gene            40393..40977
                     /locus_tag="VN178_15085"
     CDS             40393..40977
                     /locus_tag="VN178_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82342.1"
                     /translation="MLPNVTIRPQELANAITNALNPFVIFTALFALVAFVQAGVYRGL
                     LYLAVESTAAAVVAGYVLSMRRRSRVGDFWISARAERLAPAVFLLAAFVALLAVLVLL
                     DAPQDLSLMTLSMGLASAAVAGITLLWKASAHCTVAGHAAAAGVLLLGPPGLVFLLVL
                     PLVLWSRVTLKAHTISQTLAGAVVGAGFAVLFLA"
     gene            complement(41040..41597)
                     /locus_tag="VN178_15090"
     CDS             complement(41040..41597)
                     /locus_tag="VN178_15090"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS82343.1"
                     /translation="MSTYSYPHTIENGAGERITFVRRVQDPAGDRLEVENFVKPGSGP
                     PMHVHTYQEESLTIQQGRLGYQRSGEPPQYAEPGETIAFEPGEAHRFWNAGEENLLCT
                     GYIQPADNVEYFLTVIYELQRESGGSRPKPFETAFLMRRYRSEFYMAEIPAVVQRFVF
                     PVQVTVGRLLGKYKKYADAPEPIRR"
     gene            41796..42554
                     /locus_tag="VN178_15095"
     CDS             41796..42554
                     /locus_tag="VN178_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS82344.1"
                     /translation="MAVYVLVGGGWLGGWCWKPVARRLREEGHDVYPVTLTGLGERVH
                     LATPEVDLETHITDVVNLVRFEDLREVVLLGHSYAGVVVTGAADRMPERISQLAYLDT
                     GPIPDGTPLIDSFPPEVREHVERQVGELGGGWRFPVPPREELATFGSLEGLDDTRLES
                     LRSRAVDQPFGTFTQPLRLENPAREELPKVGILCSFSLEEVRAIIDSGNPVFREMASP
                     SWRFVELPTGHYPMFSRPEDLASVLLDLSSGTST"
     gene            complement(42706..42778)
                     /locus_tag="VN178_15100"
     tRNA            complement(42706..42778)
                     /locus_tag="VN178_15100"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42742..42744),aa:Glu,seq:ctc)
     gene            complement(42847..>43783)
                     /locus_tag="VN178_15105"
     CDS             complement(42847..>43783)
                     /locus_tag="VN178_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWS82345.1"
                     /translation="NLEDFVIMKSSNTPTYNFAAAFDDAEMEISHVIRGDDHLSNTPR
                     QILIHEALGNPLPAFAHVPQVLGPDKKKLSKRHGAASVEDFAERGYLPEALFNYLALL
                     GAGYAADEEIFAPEELAERFRIDRVSGNPAILDEKKLRSVNAVYLRRKSTEELAAIAA
                     PMLVESGAATEDELERDPARLAEIMDLLKERIALTTEIPDSVGYFYGGPLDYDEAEFE
                     KQFGKEFVRENFPELVERLGALPEWTEDSIEEAVRGLAAEKEKGARHLIHPLRFAVTG
                     RTVSAGLFETMQLLGGERSLLRARDAAEKMQRLPV"
BASE COUNT         7958 a        13826 c        14166 g         7833 t
ORIGIN      
        1 cgagcacggc ttcttcatag ccgccggcga cttctacgcc tcgacgctcg ccgaaaagct
       61 cggcatcggg gacagtggag ggttcatccg cgccggcctc gccccttaca acaccgaaga
      121 cgaggtcgag aagttcgtcc acacgttgga cgggatcgta cgcgaaacgt gagatccggc
      181 ctacgaaact tgggatcgtc aaaggatcac cacgttcacc tacatctcca aaggcgatag
      241 actcgagata tgaagcgggc gaccatcacg ctgcctgagg agctcgaaga tcatgggggt
      301 aagggactct tctcggttgg cgagcttggt gtaacgcttg accgaggaga gactgatacc
      361 gaacagtcgg gcggcctcgg acttggaggt gctacgctgc tcgacggcct gcactacctt
      421 ctagcgtagg tcttccgaat aggctttcat tgccacatca tcgaccatag accctgccta
      481 ggtcaatagt tgtgatcgtc gctgtaaaag cgtaacgcag cttagagatt cggtgttata
      541 ggaagtcttc agggtcccgg acgaaggctg aaagctagct ccggctatcg cgtgcggcag
      601 gcagccgaga agccgcgctc acgtcagttc gtcaaggccg gggggttcag gcagcacctt
      661 accattgagg acgccaagaa aggggatacg acgcacatgg atctcactcc gggagcaggt
      721 ctaccggact gagggctgac gtacctagca gcacaatgta taatgccgct atgtacatca
      781 gagttggagt tcgttagcgc cgcgcgaggt gcgaaatcat ggagatcaac aaggacctga
      841 tagccgcttc ctcgacgccg attgtcctgg cgatactggc cgaaggggac agctacggct
      901 atgccatcct caagcgggtt cgggagttgt cgggagggcg catggagtgg acggacggga
      961 tgctctaccc cgttctgcat cggctcgagc ggctcggcca cgtcgaggcg cggtgggaaa
     1021 tcgcggagag cggccgccat cgcaagtact atcgtatctc gcccgggggg cgggctcagc
     1081 tcgccgagga acgcaggcag tggcaagcgg tggacgcgac gctacggggc atctggcggg
     1141 cgcactccct tctcgtcccg gacggccacc cggcggtagc cgcgccacgg ggggctgagt
     1201 gatgcctgct ccggatgacg cggtatcggt tgaagagcag atcgcccgat ggcggagcta
     1261 cctcggtcgt cggcaggcga tccatcccgt cgacgtggcg gagcttgagg accacctgcg
     1321 cgagcagatc gtggtgctcg ccgaggcggg tctcaccgct gatgaggcgt tcctggtggc
     1381 ggtgaagcgc atgggcaagc tcgacgccct ctcacgggag ttcgcgcgcg aacactcgga
     1441 ccgcctctgg aagcagctcg tggtgtctcc ctctgacacc ggggggcagt ggcccgcggc
     1501 gcgagcggat gccatcgtcg tgttctgtct tgcagtagcg gcggccgtgg tgatcaaggt
     1561 gccgactatg ctcttcggga ttcacttaga ccaggacgcc ggattctacg cccgcaatct
     1621 gagcctcttt gtgctgcccc tgctgaccgg ctatttcgtc tggaagcggc gactcgacac
     1681 caccactatc cgctggctgg cccttgcgtt cgtcgcggcg ggcgtgttcg ccaacgtcta
     1741 tccgttcgcc acggaaggtt acaccgagac gctcacggcg ctgcacctgc cgatcgcgct
     1801 ctggctggtg gtgggcatcg cgtacgtggg cggccggtgg agcgaagtgg cgggccgcat
     1861 ggacttcata cggttctcgg gcgagctgtt catttactac gtgctgatcg ccctcggcgg
     1921 cgccgtcctc atggcgtcca tgaggatcat attccagtcc atcgggatcg acgtcgagcc
     1981 cttcttcgag tcgtggctgc tgccgtgcgg agcggtgggg gccgtcgtgg tcgcctcctg
     2041 gctggtggag gccaagcaga gcgtgatcga gaacatggca ccggtgttga cgcgcctatt
     2101 cacgcccttt ttcgccgccg tgctggtcgc gtttctagtg accctgctgt ggaccggacg
     2161 cggggtcgac atcgggcgga acgccctcat cgccttcgac ctgctcctgg tggtggtcct
     2221 tggccttctg ctgtactctg tctccgccag ggacccccgc tcaccccccg gcgccttcga
     2281 tgtgatgcag gtcgttctgg tggtcagtgc gctgttggcc gacgcggtag ctctgtgggc
     2341 catcgccggg cggatcaccg agttcggctt cagcccgaac cgcgtggctg ccctgggcga
     2401 gaacgtgatc cttctgatca atctggcgtg gtccgccgtg ctctacctcc gctttctgag
     2461 agggcgcgaa tccttcagcg ccctcgaacg gtggcagacg aattacctgc ccatctatgc
     2521 cgcatgggcg gcgatcgtcg tgatcgcctt tccgccagtg ttcggataca tttgaggggt
     2581 gccgatatgc ccgttctccg caccggccgt cgcggccgtg gtgctcgccg tggtgcgagt
     2641 agttcgtcgg ctccgggtag ggtgcaacag cgagatgcag ctatgggatt tcacttccct
     2701 cactgcgccc tcattcactg tccacctacg gttaccacgt cagggccgtg gccgtttcga
     2761 agtaccgtag aacacaagac gacaaaagat cgcgactttt cggcgcagcg tggccgtaag
     2821 tggcgccagg ggagccgttc gacgctcatg tgaagcgcta acctccaggg agaaataacc
     2881 tacgaacttc atagaaggtt aagaatccga tattcacttc gacagtgttc actgacgccg
     2941 agtcggtcgg gacaacggcg ccggctggtc cttcgcccgc gtgctcgggc gtggagcggc
     3001 gcacggagag gcgccgggtc tgtagaatcg gagggcatgg aggagggtag gcaactatcg
     3061 gctcctacgc tgctcaaggc ggccctggcg tacgccgggc gcggcgtgcc cgtgtttccc
     3121 tgcgaacggg gggccaagag gccgcttacg cgaaacggcc actgggacgc caccacggac
     3181 cgtggagtga tcgagcaatg gtggaggcgg cagccttctg ccaacatagg gcttccgacc
     3241 ggcgagaaga gcggcatcgt ggtcctggat gtggacgtcg acgacggcgg gctgggaagc
     3301 ctcgcgaagc tcgagcgtga aggcgccccg ctaccgaaga cggccaggac acgcaccggg
     3361 ggagggggtc tccacctctt cttccgctac ccgaggggga cggagatccg caacaccgcg
     3421 gggctgctgg gtccgggtct cgacgtgagg ggcgagggcg gctacgtggt ggtgccaccg
     3481 agccgcacgc agggccctta cgagtgggtc gaccgttcgc ccctcgccga ggcgtcgtgg
     3541 ctgatcgatc gcctgaggga gctcgacggg gccaggctgt tctgaggtgc ttcgtcaccc
     3601 ctggggcctc ggtcgcgcga ggacgaccgg cgcatcttta gccttggccg gcgagccagc
     3661 ttgccgtttc gcgcgctatc gcgggcgggc ccacgtggcc gccgtcgaac tcgcggtagc
     3721 gtacttcgta tacggcgcgt tcgagccgag gcacgatcct gcggctgcag gagtcgatcg
     3781 ggagccaccc gtctcgggtc ccgtgcgaga cgaagacgcg cggcgaccct ctctgacaag
     3841 gaactacgcg gcaatcgtct tggacttggc agaatcgcca ttacccctcg accgagggcc
     3901 ggtatgcgag ggagacgacg cccgtgccga atgtcttcga atcgaccagc gtcatcccta
     3961 ctggaactcc ccgatcttcg aagaggcgct tgccgccacc caggatcacg gggtgaacca
     4021 ggagcctgag ctcgtcgagc agcccctcct cgagcagcga cctgaccagg gcgccgctgc
     4081 cgatgatcgt gatgtccttg cccgcccgcc gcttcagtcc ggcaaccccc tccgcgacgt
     4141 caccttcgac cagggtcgag ttgttccacc ccaagggttc ctccagagtc gtggaaacca
     4201 cgaacttggg cacgctgttt atgtaatcca ccatcggcgt gccggcgggc tggttcggcc
     4261 agtaggcggc caacccctcg taggtcatac gtcccagcag catcgcgtcg gaggcggcca
     4321 tccccgccgc gttcgcctcg ttcatctcgt cgtcggcgta ggcgaatgcc cactcttccg
     4381 gcctctccat gacgccgtcc accgagacca actccagagc gaccactttc ctcacgaaaa
     4441 cctcctctgc ccgatcccac atatcttcac cttcagtgcc ggcggaacct gctgcttccc
     4501 gtcctggcga gttccgcacg ctcctcgtcg aattctcacc gaggttgtct gtgatgatat
     4561 tgccggtcat gtaggatgac atccaccgaa aggcagagtc gcctagatct ctcggaaatt
     4621 ccctcgcccg acacgtagcg tcgcccggga ttccgcttga ccaggggtgt cccgcaagac
     4681 actgaaggcg atccaccctc cacccgcgag cgccaagagt gctcccgcga ccgaacccac
     4741 gagtgggaac ggcccggaga ccagcggggc gtgaacggcg atgagtaccc ccgtccaccc
     4801 tggcaggacg cttgaccgcc agatggcgat gccgaagagg acaaagcccg ccgaatagag
     4861 caggatgctc acgctctcta ccgtttccaa gggacccgcg aagatggagt cgactatcct
     4921 tatgctctcc gggttgccgt ccaggaacga ccggctaagg gccggaacgg cgtaggcgaa
     4981 gacgcccagg cgcgaaagca tcaaggctat gccgacgatg ctcgacatca tggcaccgag
     5041 cgccagggtt ctctgcccgc cgttcgccag atatgcgtac agggccacga caccgaagat
     5101 caagacggtc agggccaaca agttgccgaa aagcacacta acgaagaagc ccgtggagac
     5161 gatgtcccgg ttatcgccgg tggccagtat ccctcaggac cccagttgtg cggtgctgta
     5221 tagacccgct aaggctagca agcccgcgag gggcagagcc agcatgccga ggcggactag
     5281 accgtccggg ttccgcaatg ccacagggta cctccctcca ccttctaacg ctcctccgat
     5341 tcctgctgca ggagtagagg gctgccgtcc tggtccagca ggatcgcggc gaagccccac
     5401 gggtagcggt acggctcccc gaggaagcgc acgccccggc cgcgcagctc ttcgtaggcc
     5461 gcgcgcacat cgtcggtgcc cagcgtcaat atcggggccc cgtcggagcg gcccaccagc
     5521 gccctctcgt ggtcgtgttc ggccttcttc aggacgatcc gggtcccagt accctcgggg
     5581 gaaacggtga tccagcgtgc gctctcaccg taggactcgt cctggacctt taccaacccg
     5641 agcttgccgg cgtagaagtc cagggccgcg tcttggtcgt tcacgaagat tccgacaagc
     5701 ggcacgtcct ttatcatcgg ctctcctcct ttgccgtttg tctgcgcttc ggcccgccct
     5761 gcggggatct gtacgccgat ggccgactcg gggccgcgac cccttcttgc ccgcgttccg
     5821 ttcaccttcg cccgctcctg gccctcagca cgaccgcgga tgccgtcata cctgcgatca
     5881 agaggaccgc gcagagggtg cccagcgtcg cggggtcggg cgagatcacg ggctggccgc
     5941 gaagcgcctg ccaggtcaag actccgacga gccccaggta cgagaggccc gccgtccaca
     6001 ccagcgtgac ccggtgtccc ggacgtagcc aggccggggc gaagaggacg agaaggtagc
     6061 cgaacagggg cagcgcctga agcccgtgca gcccgacgaa gtgcggtacg cgcagatcgc
     6121 cgccggtggt gctccaaccg gtcacgggca gtccgggtcc tccgtccttg acgcccacgc
     6181 tgtgggcgcc ctgtataggc cattcttcga tcccggcctt gtccgccgcc cgctcttggg
     6241 ctggggtctc ctgggtcatc gggaaggcga ccgccatgcc tacgagcgac acgaggatcc
     6301 cgagtctcaa ggcccaggcg aaggcggggt caggcatccg ctggacgagc agcagcgccg
     6361 cggcgagcag gttcgccgcc cagatcaacg ccacgaaggc gctcatcgtg taccagacgg
     6421 cggcgtgggt cggcgtgctc acgttgaagt ggctggtcgt gcccagcgcc gccgcgccgg
     6481 cgatcaggac catctcgacg aaaagcgcga tggccgagac ccaggagatg agggccacga
     6541 gacgcggacg gccccgcacg aaggtcagca tccagagcag cgtgaagcaa tagatcgaga
     6601 tggagacggc gaacttcatg ggcttgagcc acgccggtgc gccggttatg acgcgctcgt
     6661 cgagaaggag cccgacgaca gccaccccta gaaccagcag catcgccgcc ccgacgaacg
     6721 tcagcggccg gctcgaagcc caggcgcgtc ccgcaaggct acgccgcacg ggcgccctca
     6781 caaactgggc cttcatgctg cgcggaagta ggtcttcgtc cctttcgacc atcgccttct
     6841 cctctctcgt tccgcaacgt acacgcaagg ccaaggccgg ggaacttatt cgcgttttcg
     6901 cgaaaggaga tgaaatagtc gtggcgatcc cggataattc aaagacccga agcaagcgcg
     6961 ccaccggaag gtcgtaggac cgatgataac gatccccctc tccaccgaag acctcacgaa
     7021 ggtgcgtctt gcccccagcc cactctggga gacggtcacg agcttcgcgg tgctggcgta
     7081 ccgcggacgc gacacggtcc acgcgccctg ggccgcccgc gcgcggcggg tcctgcccgg
     7141 aaccgacctc tcggcgctgg tcaacgcgat gtgcctgcag agggcctgtc cggacttcct
     7201 taccccgccc ccagacgcct cggtcgcgag tttcgacgag gagctcgaac gcctgagggc
     7261 cacgcccgcg gaggtcgtgt acgaagaggt ggagtcgctc gtgctggtcg agaaggatct
     7321 tttcggtctc tcgcccgaga aggagcggat gctcgggatc tacctgaagg accccgaggg
     7381 gtccctgagg cggctggtgg acaccctgaa ccgctaccac gagctggcgg tctcacccta
     7441 ttggccgcgg atgcgcgagc acctggaggg ggacactatc aggcgcggcc aggccctcgc
     7501 cctcggcggc gtcgaggcgc tgctctccgg cctgagcccg aaggttaact acagcggagg
     7561 cgccatagag ctggccaaag cctacgagag gatcgtagat cccaaagggc gtgggatcac
     7621 cctggtcccc tgcgtcttcg tctggcccaa agtagacgtg ctcgtccagc ccggctacca
     7681 gccgaccctg gcgtatccgc cccgcggggc ggcgaacctg tggaccgcct cctcaccgcg
     7741 agagtccagc ggaacggcac tgggggcggc gctgggcgtc agccgggcgt cggtcctgaa
     7801 gtgcctgctc cccgctccga cgaccaccat ggagctctcc cagcagcttc ggctcgcccc
     7861 cgcggcggtc tcggcccacc tctcgcggct caaggtggcg ggtctggtcg agccccaccg
     7921 cagcggcagg aaggtctact accgcctaag ctccgcgggc gagtcgctgc tggagatttt
     7981 cggcgagacc ggctgagggt catcggagcc gcgaaggagt cgcccggtcg atcggaacac
     8041 agcgtccctc cttctctact tttgcctctt cgagcatcta cgtgttcggg tccgggcatt
     8101 gccccgctat gcttcctggg gtgcgaagct tgagcggtac gggaggcatg cggtggcaaa
     8161 gctgatctac tccaccatca tctcgctcga cggctacgtt gccgacaaag atggcgactt
     8221 cgactaacct tcattcaggg cacccaggaa aaatatggct tgctgaagcg ccggggctct
     8281 tcacgtggca tcatatgttc tctggggtga caacttcgaa ggaggagaca aacagatgag
     8341 catctttcct accaggatcc ttctggccac cgatggttcc gaggaggccc gcctggccgc
     8401 aaccaccgcc gccgatctga acaagagcac cgactctgag ctgcacgtcg tgtacgtcgg
     8461 agagttgagg ccgacctttc tggcccagac cgaacaggaa cccgccgaag tcgaacgcga
     8521 ggcgcagcgg ctgctcgatg aacaggtaag aagagtcgag gaggcaggag gcaccgtcga
     8581 agaggcgcac ctgaggttag gacgggcaga cgaagagatc gtcgacctgg ccgatagcct
     8641 gggcattggc ttgatcgtga tgggcagcag gggacatggg aggataagga gggccctgat
     8701 gggcagtgta tctgactcgg tggtcaggca cgcccactgc ccggtcacga tagtcaggga
     8761 gtaagtgaca gcgcgactcc ctggaaaaca taccttcacc aacgttggat gaataacata
     8821 ggacgttgta ctgagggaag cgagcgaagg gaggaagacg tatgcccgac aacgagccag
     8881 acaacccggg catcaaggtg cctccacctc tgatctacct cctaccattg ctcttgggtt
     8941 tgatactcca tacaaaggca cacctcccaa tcttgccgaa cagcgtggtg cgcatggtcg
     9001 gctggccact cataggttgc ggggtgctga tcgggagctg gtggcgcaag accatgaggg
     9061 acgccgacgc gcctgtacgc accgacaggc ctgttcctag gctgaccacc gcaggtcctt
     9121 tcggttacag ccgcaaccct gcttacctct ctctggccat gatctacgcc gggacagccg
     9181 ccctgaggaa ctcgctgtgg gcgatgctct tcctaccgtt ggttctgtat gtgatccagc
     9241 gcgaagtgat cgggcgcgag gagcgctacc tcgaacgcac cttcggggaa gagtacctgg
     9301 cctacaaggc gagggtacgc cgctgggttt agatcatggc cgtcagatct tccgagagtg
     9361 tgacaaccct ctgcgagatt ctggtataat cttcgcctgt aacgtaaggt gggtttacag
     9421 tagacggagc gccaccccgt gagatagagg gtggcttttc tgtttagtgg ggcccaggaa
     9481 catgaaaggt gggctccggt atatggccca aggaacggta aagtggttca gtgacgagaa
     9541 gggctacggg ttcatctcgc ccgacgacgg gggtgaggac ctcttcgtgc accacacggg
     9601 catagcaggc gagggcttca agaccctcga cgagggcgcc aaggtgacgt acgaagcgac
     9661 ccaggggcgt aagggtatgc aggcggagaa cgttactccg gcgtagaacc cgaaaagcaa
     9721 gagaacgcaa aggccggggt acaaaccccg gcctttgtca tgcgtagggg cgacgcaatc
     9781 caacgtaggg gcgggtttcg aacccgccca atccccgcga accgcccgag atcaacccat
     9841 caacaggtac agcagcgcct tctgggcgtg cagacggttc tcggcctggt cgaagacgcg
     9901 gctcctcgga ccatctataa ccccggccgt cacctcctga tccctgtgcg ccggcagaca
     9961 gtgcaggaag atggcgtcct ctgcggcgag gttcattagt tcctcatcga cctgataggg
    10021 ggcgaatttc tccttacgct cttcggcttc gtcctcctgg cccatactgg cccatacgtc
    10081 ggtgtagacg actctggcgt ccctgacggc ctcgtcgggg tcatcggtaa ggtgcggggc
    10141 ggcgcccaga gatcctgcca gctccactat ctctctttcc ggaccgtgtc cttcggggtg
    10201 ggcgatggtg agttctgcgc cgctcagggc gcaggctatg gcgagggagt gggccacgtt
    10261 gttgccgtcg ccgacgtagg agatcttcgt gccttcgacc gtaccgaact cctcgcgcac
    10321 ggtcatgagg tcggcgaggg cctggcacgg atggtgggtg tccgagagcc cgttgactac
    10381 agggacgtcg gccgcgccgg cgagttcttc gaggtccccg tggtcgaagg tgcggaccat
    10441 gatggcgtcg atgtagcggg ccaggacgcg gcccgtatct gacggcgtct cgcgtttgcc
    10501 gatctggatc tcctctgggc tcagatgtag cgcatggccg cctagctggt acatcccgac
    10561 ctcgaaggag acgcgggtcc tgtttgatgg cttctggaag accatcgcga gcgtgtgccc
    10621 gcgcaggggc ctgtagggga tatggctctt ctgcagcgct tttatccgaa cggcctcgtc
    10681 caggatcagg gacatctccc cgggggtgaa ctctccaagc gtcaggcagt cgcggccagc
    10741 gaggggggat gggggagggg cgttctggag gtcagggatc accatgctca cgctaccgcc
    10801 gcctcgggca cagtctcctc tacggtctgg tagatgccgc ctcgaaaggt atcgagcgca
    10861 gcccgaacct ccgttctgct cacggtgagc ggcggcgcca tacggatggt cgttccgccc
    10921 accaggttga cgaggacgcc atgatccaga cagtgctcga agacctccgc ggcaaggccg
    10981 gcgtccccga agtcgaaccc gagcaggagg ccttcacccc gcacctccgc gccggggatc
    11041 tgctccgcca gcttttggag agcgttcttc aagacggtcc ccttgaaacg tacttcttcg
    11101 aggaaggcag gatcgttcac cacgcccagc acggccttca cggcggccat cgccaggggg
    11161 ttgcccccga aggtcgagcc gtggttgccc ggccccagcg cggcgtactc ttccttgacc
    11221 atcatcgcgc cgaccgggag accgccacca agacccttgg cgctggtgag cacgtctgga
    11281 acgacgtccg tcccctggta ggcgtacaga tatcccgtgc gtcccgcgcc ggtctggacc
    11341 tcgtcgaaga tgagcagcgc cccgtactcg tcgcaaagct cgcgtaggcc cctgaggaag
    11401 ccttccgggg gctcgttcac gccgccctcg ccctggatcg gctcgacgag cacggccgcg
    11461 gtgtggggac cgaccagcgc ctctgcggct tcgagatctc cgaagggggt atggaccatc
    11521 cctggcagca tcgggccgaa gccgtcttgc agcgcgggct gggccgtggc tgtgaggccg
    11581 ccgtaggtgc ggccgtggaa ggacttcgag aacgtcagga tctcgtgctt gttgggaccg
    11641 taggtatcgc gggcgtactt gcgcgcgagt ttgatcgccg cctctacgct ctcggctccc
    11701 gagttgcaga agaagaccct gtcgaagtcc gtcgcgccgc agagcatctc ggccacctct
    11761 gcctgaagtg ggatctcata caagttggag caatgaatga gcttccctgc ctgctcctgg
    11821 atggccccca cgagcgccgg gtggccgtgc cccagggagt tcgtggcgat cccgcctata
    11881 aagtccaggt accggttccc ctcgctgtcg aagagccagc ttccttcgcc ccccgttggc
    11941 gctatcccca gacgtttgta agtctccatc actgcttgca tgccgcgact ccttcggata
    12001 tgagtgttcc aacccgctcg ccggcgacag ccgccccgag cgcgcctttc ctgttgccgt
    12061 tgaggatcct ggcctccacc ctgccgcgcg cggccgaggc cgcggcccgc aacttgggca
    12121 ccatcccgcc cgccgcgagt ccgctgtcga cgtaggcctc gcacccatcc gggtcgagcg
    12181 tctccaccgt ctcgccctcc tccagcagcc cgtctacgtc ggtgagtagg aaaaggtgtc
    12241 cagccccgag cccgacggcg agcgccgccg ctgccgagtc ggcgttgacg ttgtacgcgc
    12301 cttcaggacc cagcccgagc ggcgcgacga cggggacgaa ccctcctgcc cacagagcct
    12361 cgatgagctg cggcccgacc cgcacgaccc tgccgacccg gcccagatcc gggaccggct
    12421 cgaccagcag cgtcggtccg tcggtccccg agatgcccgc cgcctggaca ccgaggtctc
    12481 ccagcccgcg cacgaggccc ttgtttacgc ccccggcgaa cacgttctcg gccacctcga
    12541 tggtctcctc gtccgtcacc ctgagcccct cgtgaaaccg cgtagcgatt cctagcgcgt
    12601 ccagcaccct ggtgagctgg agaccgccac cgtggacgat gacgaccctg tggccggccg
    12661 aaagcagatc gggcaggtcc tccagcgccc ctccagcaag gatgccgccg ccgatcttga
    12721 tcaccacggg cctcacgtcc tgtagctccc attgatcctg acgtactcgt aactcaggtc
    12781 gcagccccag gcagcggccg aagcgtcgcc ttcactcagc cggaccgtga tcttcacttc
    12841 tcccgcggcg agagtcgcgt tggcctcggc ctcctcgtgc ggcacgggct ctccacccga
    12901 gaagaccttg atcggcccga accagagctc tactccttcg ggatcgaaaa cctcccccga
    12961 gtaacccatc gccgtgagca cccttcccca gttggcgtcc tcgccgaaga ctgccgcctt
    13021 gaccaggtta ctccccacca cgctcttggc tagcgccgcc gcggactcct cgctcgcagc
    13081 gccctcgacg gccacctcga cgagcctggt agccccctcg ccgtcgcgtg cgatctccct
    13141 ggccaatgtc cgcgcaacgc cctcgacagc ttctgcgaag gccggatagt ccggcgagtc
    13201 cagggtgaga ggctcgttac ccgcggcgcc gttcgccatg aggagggcca tgtcgctcgg
    13261 ggaggtgtct ccgtccacgg taacgcggtt gaaggtgcgg tccgtgaccc ggcttagagt
    13321 ctcattcagg cagctcttct cgacggcggc gtcggtcgtc agaaacgcga gcatcgtgcc
    13381 catgttcggg tggatcatac cgctcccctt tgccgttccc ccgaccgtga cggtcctgcc
    13441 cccgatctcc acccgcgcaa ccgcctcctt tgcacgggta tccgtggtaa gtatggaccc
    13501 ggcgaagccc tcatcgccct cacccagctc gctggacgcc gcacttatac cggatgagat
    13561 cctgtccatc ggcaaccgga cgccgatgac gccggtagaa gctaccgcaa cctctccggc
    13621 ctcgagcccg agcgcctcag cccctagagc ctgcatcgcg cgggcgtctt cgatgccccg
    13681 ctcgccggtc gcagcattgg cgttgccact gttcgccacg accgcccgca cgccaccgct
    13741 ctccacagcc tctcgcgtca cgactagcgg cgctccccag acggcgttgc gggtgaacac
    13801 ggccgccgtc tcgcagggaa gctcggagaa gaggagcccg agatcccgcc tcccaacgtc
    13861 gcgaacccca cacccaaccc cagccgccac gaaccccgag gcagccgtca cgccaaagga
    13921 gggagacttt attctgattg ttgaatcttt attcatcacg ggggcatatt gtcgcacccg
    13981 cccgtgaggg cgtcaaccgg tttatgcaga aacttgaata aatatccgaa ggttctacaa
    14041 tgggcggcat gggactgaga gtcggcgtgt atggcgggag tgggttcgcg ggtatcgagc
    14101 tcgtgcggct gctcgccggg caccccgagg ttggggcgat gagcgttgcg tcgcgcggtt
    14161 atgtagggtt agggatcggg gaggtgtatc cgcagctcgc cgtcgatgga acctatgtgg
    14221 cgccggagga gatcgaagcc tccgacctcg acgtggcctt cgtggcctac ggtcacggcg
    14281 agagcgctgg ggcggtaaag gagttgctgg ggtcggggac tcgcctggta atcgacctct
    14341 ctgcggattt ccggttgccc caggaggagc tatatgtaga gtggtacggg gagcatcccg
    14401 cgccagagct tctcggggag gcgcactacg ggttgcccga ggtcttcggg gctactgagg
    14461 ggaggctcgt cgccaatcct ggttgctatc cgacggcggc cattctggct cttgctcctg
    14521 tggtgaggcg tatgggggcc gggatccgct ccgttaccat caatgctctt tccggggtga
    14581 gcggggctgg ggcgaagccg agcgtgaaga cgcacttcgt cagcgtgaac gagagtgcgg
    14641 gcgcctatgg ggcgccgcgg cacaggcaca cgcccgagat cgagacgatg ttgcggcgcg
    14701 tgggggagac gcccgaagtc acgttcgtac ctcacctcat ccccataacg cgcggggagc
    14761 tcgagaccat cgtggtcgat gtgggagagg tgcctgaggc ggaggaggtc ctcggctggt
    14821 atgaggagga ctacgagggg tgggccttcg tcgaggcgcg ggaggagtac ccgcgagtcg
    14881 cgcacgtggc gaacaccaac cggtgcaggc tctcggccgc ggtagatggc cgggcgggga
    14941 agctgttgct cttctcggcg gtggataacc tgctcaaggg cgcttcgggg gcggctctgc
    15001 agaacatgaa cctggcgctc ggctacgagg agtacctcgg gctcgagcac cttacgtagg
    15061 aggacggttt tgggagcgac gatgtcaccc gtggacaaga cgacgaggca ggatctgata
    15121 ctgcgtttga tctcggagcg cgacctcggc acccagcagg atgtcgtggg ggccctcgcc
    15181 gacgaggggg tcgaggtcgc gcaggcgacc gttagccgcg acctcgccga gctgggggtt
    15241 ctcaaggtcg ggaaccgcta cctggcgttg ccccacgacc ccggctccgc gggtatcgag
    15301 gtcctcccca gcttcgtgct cggcctgatg cccgcacaga accaggtcgt catccaaacc
    15361 cgcgacggga ccgccggcgc cgtctccagc gtcctggatc gggtgaaggg cctgaaaata
    15421 gtgggcacca tcgcgggcca ggataccgtg ctcgccatag cgccggacgc agccgctgcc
    15481 accgaggtgg ctaacctcat cgccgatgta tgccgggcga agacgcggcc cgccggcaac
    15541 gtcgagtagt cgaaggcctg accttcgggg accattgcgg ccgaaggcag gcccacccta
    15601 acgccacagg cacatttcag gaggaaaccc atgctagaag gtaagaaggt cgtactcgcc
    15661 tactcgggag ggctggacac ctcggtctgc gtcaagtggc tcgagcagca gggggcggag
    15721 ccctacgcgc tgtacctgga tctcggccag ggagaggctt cggaggacgt gaaggcgaag
    15781 gctcttaaga taggcgcctc agacgccttc gtgcgagacg cgaaggccga gttcgccgaa
    15841 gagtacgtcg cacccgcgat caaggccaac gcgctatacg gtgggaagta cccgctcttc
    15901 acggccctcg ggcgcccact cattgcgaga aagctcgtgg aggctgcgag agaagtcggg
    15961 gccacgcaca tcgcgcacgg ctcgaccggg aagggcaacg atcaggtccg cttcgacgtg
    16021 acgacggcct ccatcgcccc agacctgacc gtcgtcgccc ccgtccgcga ctggaacatg
    16081 agccgccccg aagagatggc ctacgccgag gagcacggca tccccgtccc cacgacgaaa
    16141 gaatccccct acagcgtgga cgagaacctc tggggccgct ctatcgaggc cggacccctg
    16201 gaagaccccg accacgaacc caccgaagac gtcttcgcgc tcaccacctc acccgaaaac
    16261 gcccccgacg agccgcagta cgtcgagctc ggcttccaag aaggactgcc gactaccctc
    16321 gacggggaag agctgccgct cgtagatctc atatccgagc tcggctccgt cgccggcgcg
    16381 aacggcgtcg gccgcatcga catgctcgag gaccgcctgg tcgggataaa gagccgcgag
    16441 atctacgaag cccccgcagc cctcgtcatc atcgccgccc accgcgaact cgaaaccctg
    16501 accctcacca gggacgtcct ccgcttcaag acggccgtcg agcaacgcta cgccgaactc
    16561 acctacgaag gtctttggtt cacccccctc aaatccgccc tcgacgcctt catcgcagag
    16621 acccaaaaga ccgtcaccgg cgccgtccgc ctcaaactct acaaaggcaa cagcaccgtc
    16681 gtgggccgca cagcccctaa cgccctctac agcgaagacc ttgccaccta cgatcccaac
    16741 agcaccttcg acgaggccgc agcagcaggt tttatagccc tctggggcct ctcagcccgc
    16801 cagtgggcaa gcgtcaacgg cagcacagaa tacaaccaga tatctccaca acattaaacc
    16861 tgtggtccac actacaaggt aaatgaagcg gatttgcagc atatttatta cccaagaacg
    16921 tgaacccggg tcagaccaca gtgtaaacgt gtacaacaag tcgagcatga acctgtagta
    16981 cagatttatg ctgacgcgtt cggtgttctg gaacggtgct cgggtttcgg gttctgtggg
    17041 attctgcgtc tggagcttct ctcctgcggt gaaagttcct gcctgtactt gacacatcga
    17101 ttttcgttga tctatacttg gatcaagaaa tcttgatgtg agaggtggtc atggaagaag
    17161 atctcaagca gagggtacgg gaccggtatg ctggtgcggc gaggagcgta ctcggggaag
    17221 ctgaggatag cgcgtcgtgt tgcggggttg cggagtctgg ggagcgagcg gcgttcgagg
    17281 tggatatggt ctgcgggagc tactccgagg ccgagaggag tgagttgccc cagctcgccg
    17341 ctgatgcttc gttgggatgt gggaacccgg tggcgctggc tacgctctcg cccggtgaag
    17401 tcgtactcga cctcgggagt ggtggtggga tagacgtttt gctctcggcg cggcgggtgg
    17461 ctcctggggg gagggtctat ggtctggata tgaccgatga gatgctcgag ctggccagga
    17521 ggaatcaggc cgaggctggc gtggagaacg cggagttctt gaagggggag atcgaggacg
    17581 taccccttcc tgacgggcac gtcgacgtgg ttatcagcaa ctgtgtgatc aacctttcca
    17641 cagagaagca acgcgtgatc tctgaggcct tcagggttct caagccggga ggccggttcg
    17701 ccgtctccga cgtggtgttt ctcggcgaga agaacaagct gccacccggt gtcgcacgga
    17761 gcgtcgagct gtggtcggga tgcgtctccg gagcgctgga gaagggcgag tacgaggcgc
    17821 tgctctcaga agcgggtttc gaggaggtgg cggtggaggt cacccacacg tatcctccgg
    17881 agcagatagc agggctctcc ggcgaagacg cggaggcact gcgtgaggtc cctgcggcca
    17941 gcgcctttat cagggccggc aaaccggagg acctgtaacc gtggcgcggc agcgattgcc
    18001 ggtcctccag cagagtagtg tcgccccagg aaccatagac gtcggtgggg aggagcgcgc
    18061 aggacggctg gtggcgttcg gccgggcgct ctcggacccg atccgggtac agatgttggg
    18121 gatgctcgcc ggagggcgcg ggtgctgcga gctacccgac tgcggagttc cggcgaagga
    18181 tcaggacgcc ggcatctgcg tctgcgagtt cgagagattc ttcgatgcgg ggcagtcgaa
    18241 gatctcctac catcttggca agctcaaagc ggcaggattg gtgcatgagg agcggcgcgg
    18301 caaatggagc ttctactcgc tcgaccgcga ggccgcagga cggctcctcg ccgagaccgc
    18361 ggccaatctg gacggagacc cacgcgatga aggctgaccg gcgcgcagaa gatcggacgc
    18421 gtgtcctgtt cctgtgcacc cataactctg cccgctccca gatggccgaa gggttgttga
    18481 ggggtatggc cggagatcgt ttcgaggtgt tcagcgcggg gaccgaggcc acacgtgtca
    18541 ggcccgaggc gatctcggtc atggccgaga tcggggtgga tatctccgcc caggagtcgg
    18601 agtcgttaga ccgctaccta ggagaaaccc tggatctcgt cgtcaccgtc tgcgccgacg
    18661 cgaacgaggc ctgcccgctc ttcccgggag cgagggagcg cctgcactgg tctttccccg
    18721 acccttcgaa agccgccggt ggccgcgaag agcgcctgcg ggatgagatc cgtgcccgca
    18781 tcgaaggcga actggcgaaa ccggggatgt gatcccaacc cggccccgtc cgtggcgaca
    18841 tcagcggaag acagactgct atcatcggct tcgtgccggc gccacccaca cacgacccct
    18901 cggagaccga cctcaccatg atgcgccgcg ccctactcgt cgccgaggag gccgcgaaca
    18961 agggtgaggt tcccgtcggg gccgtcgtcg ccaggggacg ggagatactg gccgtcgccg
    19021 ccaacgagcg cgaggcgaca ggggatccta cggcccacgc tgaactcctc gccatccgtc
    19081 gggcatccgc cacaacagga ggctggcgac tgacgggctg caccctctac gccaccctgg
    19141 aaccgtgccc catgtgcgcc ggcgccgccc acgcaagccg tctctctcgt ctggtctacg
    19201 cggccccgga tcccaaagcc ggatacgccg gcaccctcca caataccccc accgactcgc
    19261 gcctcaatca taccctcatc gtcgaaagcg gcctgctcga agagagtgcc gccgcattgc
    19321 tccgcgattt ctttcgatac cgtcgccgga gacccggaga ggcatccgaa agctcccttg
    19381 cataggtgcg acgatttact agacactata cttaaactta aggtatatgg ttctttacat
    19441 actaatctta gtctctaccg agactagaag gccatctgta gacctgaaca taacggtgcg
    19501 tgatttgcgg catattcccg gatattgcgt agttctgaaa aattctttga aaaattctgt
    19561 agatgaggac atgggcgagt attccgctaa cgaaggggca ttcgagcgtt tagtgtcaat
    19621 ttaacctcaa gtaaaggtga atcgcagggg tattgcatct cgaaaatatt gtgtgctagg
    19681 ttttcacggt accagatgtg gtgttggcgc gatgggggtt atgttatgca gtgtccctac
    19741 tgtgatttcg aggacacgaa ggtgatagat tcgcggcttt cggagacgaa ggatgctgta
    19801 cgcaggcggc gagagtgtct atcgcgctcg tgcgggaaga ggtttacgac ctatgagcgc
    19861 cgcgaaccgt tgaggctaat ggtgatcaaa cgcaatggcg ggaaggagcc gttcgaccgg
    19921 gagaagttac ggtcggggat ggcgaaggcc tgtgccaagc agcaggtcac tgacgagcag
    19981 gtagagctca tcgtcgatga gatcgaggcc gagctccgcg agcggcggcg gcacgaggtg
    20041 acctcgaggc gtctcgggga catggtgctc atgcggctca ggaggatgga catggtggct
    20101 tacctgaggt tcgcttccgt gtacaggcag tacacggacg tggatcagtt ccgcaccgag
    20161 ctcgtgcgtc tggcgggctc ggggatgggc aacggcaggt agggaccagg gcgcccgcga
    20221 tcgggggtaa tgcgggcgaa ttcggggatg gaataagggc aacgagcggt agaatgggaa
    20281 ggatgggtat ggaggtagac cgtaagaggc tggcggagat agatctcacc gagaacgcgc
    20341 gcgccgttct ggggaagcgc tacctgaaaa agaacgagaa gggcgagcct gttgaggagc
    20401 ccatcgagat gttcttgcgg gttgcgtcga acatcgccga gggcgagttt cgtttcaagg
    20461 agggtgaaga ggcccagggg ctctacgagg agtcgaggga aaggttcctc cagatcatgc
    20521 tcagccggaa gtttatgccc aactcgccga ccctcatgaa cgcgggcagg gagctgcagc
    20581 agctttccgc gtgcttcgtg ttgccggtcg aggactctat cgacggtatc tacgacaccc
    20641 tcaagcacca ggccatcatc cacaagagcg gcggcggcac gggcttcggc ttctcccggt
    20701 tgcgtcccaa gaacgatctg gtgaagtcga cgatgggcgt ctcatccggt cccgtctcgt
    20761 tcatggccat atatgacgct tcgacggaca agatcaagca gggcgggacc aggcgcgggg
    20821 cgaacatggg tatcctaagg gtcgaccacc ccgacatcga ggagttcatc gactgcaaga
    20881 gggacaacga ccagatcaac aacttcaaca tctccgttgc cataaccgac gagttcatgc
    20941 aggccgtaga ggctgacacc gacttcgagc tcaagaaccc ccgcgacggc gaggtcatca
    21001 agaccgttcg cgccagggac ctgttcaaga agatcgtcga gggagcgtgg ctcaacggcg
    21061 agccaggcgt cgtgtttata gacaagatca acgacgacaa cccgacgccg cagttcccga
    21121 tagagtccac gaacccctgt tcggaagcgc acctgccgcc ttatgactcg tgcaacctgg
    21181 gttccatcaa cctggagcgc ttctaccgcg aggatacggg agacgtcgac tgggacgagt
    21241 tgagggagac ggtgcacacc gcgatccgct tcctcgacaa cgtcatcgag cagaacaact
    21301 acccgcttcc cgagatcgac gagatgagcc gggggaaccg taggataggt ctcggggtca
    21361 tgggcttcgc cgatctcctc ataaagctcg gtatcaccta cgactcggaa gagggcctcg
    21421 ccttcgccga gcggataatg aagttcgtgg acgacgaggc gtgggaggag agcaggaccc
    21481 tcgccgagga gcgcggcgtc atgcccaact acgagggttc gcgccacgag gcgcggggcg
    21541 acagggtccg caacgcgacc gtcaccacga tagccccgac ggggacgatc tccatgatcg
    21601 ccggctgctc gggcggtatc gagccgctct tcgccgtggc cttcatgcgc cgccaggctg
    21661 acatggagat gcccgacgtc aaccccgagt tcgtcaagct cgccagagag cgcggcttct
    21721 actccgagga gctgatgaag aaggttgcgc agcacggctc cgtgcgcgac gtcgaagagg
    21781 tcccagacga cgtgcgcagc gtgtgggtga cctcccacga cattagttcg gagtggcacg
    21841 tcaggatgca ggccgccttc cagaagtaca cctcgatggg aatctccaag accatcaatc
    21901 tgcccaacga ggcgacgcct gaggacgtgg aagacgccta ccgccaggcc tacgccctga
    21961 actgcaaggg gatagcggtc taccgcgacg gctcgcgcga tgcccaggtc ctgtccacgg
    22021 gcaagacggg cggggagaag ccgaccatga cgccggcctc ccccgaggcg ccgacgtcgc
    22081 cccccgttac cccaccggcc ggcgcggaag cccaaccgca gaacttctcc tcgctcgccg
    22141 aggcgcgcaa cgggaggccg cgcaccctga ccggcgtgac caagaagatt cgcaccggcc
    22201 acggctcgat gtacgtaacg atgaacgacg acgagttcgg gcccagggag tgcttcgtca
    22261 tactgggcaa gccgggcggt acggccgccg cgttctcgga cgcgctcggg cgcatgatca
    22321 cgatggccct tacccatggc gccacgcccg ccgaggtggt ccaccagctc cgcggcatcc
    22381 aggacggcca tcccgccggc gtagggccga acgcagtcct ctccgtcccc gacggtgtgg
    22441 cgagggccat gtccgagaac tacctgatcg aggacaccct gccggacacc cgcgagctcc
    22501 cgatcatcgg ggcctgcccc gactgcggca gcggcctgac ccgccaggag ggctgcgtcg
    22561 tctgccactc ctgcgggtac tcgaagtgta actagaatac cgacggcggc ctgccaggga
    22621 cggacctacc cggtccgtcc cttttatgcc tctgtatccc ggcgttccct atccaggctt
    22681 tgatcttcga aagacgcggg gtcttccgag ggttcgatgt ggataaagaa ggtgctctgg
    22741 ggtaacgcct cggcgacctc gccttctatc ttctcggaga ggtcgtggcc ctgctgcacg
    22801 gtccattggc cgggcacgag cacgtgcatc gagacgaagc ggcgttgtcc tgacgcccgc
    22861 gtgcgcacgg cgtggaaccg tatcccctgg ctctcatagt gggacaggac tctggagatg
    22921 acttcctggt cctcttgggg cagggcccgg tccagcaaac cttgcgcggt atcacgcagc
    22981 aggcggaagc ccgtccggac gatgttcgcg gcgacgacga gcccgatcag ggggtcgagc
    23041 acgagccatc ccgtgagctg caccatcacg accccgagga cgacgccggc agaggtccat
    23101 acgtcggtga gcaggtgctg cgcgtcggca cgcagcgtta tcgagcgcaa cctccttccc
    23161 gcccgcagga gcacgagcgc gacgatcccg ttgagcgccg cggcaactag cgtgacggag
    23221 aggccgagcc ccacgttctg aagcggttgc gggtccatca gccggtccca cgccgtaacc
    23281 ccgatccagg ccgctgcgat gattatcagg gcgctctcca gcccgctgga gaaatactca
    23341 gccttgttgt ggccgaaggc gtgctcttcg tccggaggac gaacagccaa ggacagcgcc
    23401 caaagtgccg ccaccgcggc cacgaggttg acgacggact cggccgcgtc ggagaacagt
    23461 ccgacagacc ctgtaagaaa gtaggcccca agcttgaggc ctatagtagt caccgcagcc
    23521 gctatcgaga gctcagcgta gccgcgcgca gtcacgccgt tcgtaacgtt gcccttcgtc
    23581 accgctgcct cacccgcgca gacgcgcgcc tcgaaccggc gagagtctca atgaatgtcg
    23641 ggaacatctc cactttctac catcttccag atagacccaa ccacaaacca cgcgacctac
    23701 aactacgccg gcgatcccaa gggtggcggt ccaggccacg ggccgccagc ggggcgacgg
    23761 tagccgaccg tcggggaaca acagcagcac gaagctcgtg gggacataat ccacatatcc
    23821 aaagtacacg acgaccgtct tctgtacgtt acgaaaagcg ttacgagcaa gacatactgg
    23881 cgcgcaacgc cgaacagaca gagcttccct gaaagttact gtcaatgtgg cgcaaacgat
    23941 cggcaaatct atcaggtccg gtctcaccgc ataaagtaca ggcatttgca gggctcttgt
    24001 ctccagtaaa gagagattgc tggtcgggat tcgtagtccg gaacgagatg acccagcccg
    24061 ttggaataaa ccggagatcg aggtacaggg ttgagggact tcatcgtaga gggggagatg
    24121 cggttgaagg agaggggcca ggtagttaca ctctcgcgcg gagaggtggc agagtggttg
    24181 aatgcggcgg tctcgaaaac cgttacgtcg gcaatcccgg cgtcgggggt tcgaatcccc
    24241 ccctctccgc gctttagagc tagtcagcac ggcgccattg gcgcattgtc agcacgcttc
    24301 ggcctgcggc ctcgcttgtc agctagtcag cttctcccat tccagcctca tcctgccgcg
    24361 cacgacgaac cgctacctgc ggcagtccag ttctgcgttc ttggcggggc cacgaaacga
    24421 cttgctgaag tgctgacgag cgacagccga aggctggagc gtgctgaaat gctgaagtgc
    24481 ttccacacgc gtatgatgct ccatatggtc tggctttgga gatctgggcg tgcataaagc
    24541 cggtgactct agggtttggg atgcggtcat gatcacggcc ggaatctggg tgcttgggtc
    24601 ctcgttgtgg atgtacatcg actacatggg cgcgaccctg gagaggatct ctacacggtc
    24661 catggccttc cacgtggact tcgacagctt ctggcgttcg gccagggcga tgctcgaagg
    24721 acagagcatc tacgatacgg gggtcgatct ggtaaacctc aatccccctc tctggaccgt
    24781 gctcatctcg cctctaggat tgctcgagcc tataacggcg tacagggtct tcgtactgat
    24841 ctcgctgcta atcgtcgttg gctatctggc atggaccgta gaggagctac gcttgagacc
    24901 ggggtggacg gtggtgggag tcctcatgct gctcctctct tcgcccctgc tcgctacgct
    24961 ggcgctcggg caggtctatc ctgtgctggc ccttggcctc gtggcggcct gggtggctga
    25021 ccgtcgggac gaacaggaga tctccggcgc ggcccttggc ctggtcgtcg ccttgaagcc
    25081 ttcgcttctg cctgtgctac tttggccgct cgcgaggcgg aggtggaggg cgctcttggc
    25141 cgcgctcgcc tccggggccg tggccacgct cattggggtc gccgttttag gacctgggga
    25201 gacgctggac tacatcgagg tcttgaggga gcaaccggtc agcgcatact gggacaacgc
    25261 atccctgccc tccgccgcgg cgcggctgtt tacggagaat ccctacgccc agaacgtcgc
    25321 gacgttgccg tggatggtcg ttgtcggctt cgcgcttggg ataatggcca tcttcattac
    25381 cgccatgagg gtgaggcacg gacccgaggt gggattgtgg gtgatggtcg ctgcctcgct
    25441 cctggcctcg cccatcgcct ggcacaacta tctggtgttg ctcgggccag gcatcctgct
    25501 gctcctggcg cggggcaggg cggcgacggc cttcttgctg ctcgctttgc agtccatccc
    25561 ggcgcagtgg cccctgatct ggaacgacag gagcacggtc gcggcatccc tggcgatgac
    25621 gctctatctg tacatcctga tcgcgcactg gctcgccctc ctggcagcga cgagggagcc
    25681 cgcgggtatg atcaggcctg ccgcagagga gccgggataa tgggagttac gagcttcctt
    25741 gggaagcgtg gcatacttcc gtgagggtag cggatccttg aggaaaggtt gaggagatgg
    25801 cagagacgct catgcagcgc gtggtcgaag cattgccctt cctggacgac atcgccgatg
    25861 acgttcagcc gaaggtgcag gaggccgtcg cagcgggcgg cacgacggca cgcgacgtgc
    25921 tcgacggcgt ctggctcgag gcgccgttgc acccggtcct cacggacgtg ccggtaggag
    25981 cctggaccgc cgcgctcgtc ttcgatggac tagacattgc caccggaaag gagcccgtta
    26041 ggcacgcggc cgacgcgtca ctcgttttcg ggacgttggg agcgctcggt gcagccgtga
    26101 ccgggctctc cgactggcgg tacctgagtg gagggtcgag gcggatggga gtggcgcacg
    26161 gtcttctgaa cgcgatcggg ctgattctta gcatcgtatc gatcatcctc cgggcttcgg
    26221 ggcgcaggaa cgccgggcgc ctgacctttc tgacggggta ctccatcagc ggcatggccg
    26281 cccatctggg tggagagctc tcctaccact acgggctaag ggtggacagg aacgtcttcc
    26341 agggtaccgg tccagatgag ttcgttcccg tcctgggcga gagcgagctt gcgcaagacg
    26401 acctgcgcag ggtcgaggtc gaaggggtgg gcgtcttact tagccgcact tccgccggcg
    26461 aggtctgcgc catatccgcc acgtgcaacc acttctccgg cccgctcgaa gaaggagagc
    26521 gagaaggcga caccgtagtc tgtcccctgc acaactcccg cttcgagctg tgtagcggcg
    26581 aggttatcga cggtcctgcc atcttccccc aatctcgcta cgagaccagg gtgcgcgacg
    26641 gcaagatcga ggtcaaggcc gccgcagaaa acattcagaa gaaggtcagg tagtcatctg
    26701 tacgccggct gagatagtgt caggcacgcc tgcggggtgg aagatgccgg cctagtgctg
    26761 agcgccttca tcttcgggtt ggtggcctcc agcgcactgg tgatcggcgc ggtcgtgggg
    26821 gcctactgga caccgcccag gcgattgctc gcggcgaccc tggcgttcgc cagcggcgcc
    26881 ctcataaccg cgttggcctt cgacctcttc ctgacatcct tcgagcgcgg cggcgcatgg
    26941 ctctcctcga tcgggttgct cttgggtgcc gcggccttcg tcggagcgga cgaggcgctc
    27001 gaccgctaca tcgcaggggc tggcagcggc gtctcgggct tcgccctgct ggcagcagtg
    27061 acgctcgacg ggatccccga gaatctggcc ctcggtgtct ctttgctcga gacgcccact
    27121 ggagggacgt tgacgcttct cgtcgccatc tttctctcca acctgcccga gtccctgggc
    27181 ggggcagtgg ggatgcgcca gcagggccgt tccagactat tcgccatcgg ggtctggagc
    27241 gcaaccgcgc tcatactcgc cgtcgcggtg gtgctcggca acgtgtccct ctccagcgct
    27301 ggagaaggaa cgctcgcagt attgctctcc ttcgccgggg gcgcggtgct cgcctcactg
    27361 gccgacaccc tgatgccgga cgccttcagg gagggcggta agtgggtcgc cttcgccacg
    27421 gccgccggtt tcctgctgtc gtttctgatc gcggaagtgt gaaagcggta ttggaaggtt
    27481 ccccttccaa taccgcgctg gtcagcacgc tccggcttcg cctccgcttg tcagcacgcc
    27541 gcctcgcttt gctcggcggc ttgtaagcat ttcagctagt cgtcctgtaa cctcgctctg
    27601 agcgtgaacc cgaccgtggc agactgctct tctcatgcgc tgccgggcgg ggccagaacg
    27661 ggagaaagct gagaagctga caagcgaggg ccttataggc ccgaagcgtg ctgacaaggc
    27721 gccaaaagcg ccgggctgac taactccttt ccagcgtctc cagggcttct gcggcggcgc
    27781 gacggaggga gcggctggag tcgacctgga agaccttctg caggtcgcca cgggctcggg
    27841 ccatctcgcc cagtgccgcg agggcagagg cccgattgta gtgggctatg ccgagtttgg
    27901 gatccagctt tatggcctgg tctgtggctt cgagagcggc gccggggttg ccagatgcgt
    27961 cgaggctcgc cccgagggcc gagtagtaac gaggtagagg acggcgggcg cagatctcat
    28021 cgtagaggtt cgccgccttc tcgtactcgc ccttgcggaa ggcggtcccg gcacggcgtt
    28081 cgtccggggt gacggcgcgg gtcatgagcg ccgtgaggac ggcggcgccg ccgaggtaga
    28141 cgaggaggac gggcccataa ttctcgacgg tcgctaggtt catcacgaag ggcagacccg
    28201 cgataaagac gcccgtggag agaggtggta tcagccgcag gaagaagtcc ctataggccc
    28261 ttacgcgcag cagatccctg ttctgacggc cgatcaagaa ggtgaagacc atcaggatca
    28321 ccagcgcgcc cagcgtcgga tagagcagct tcaagatagt tcttccagca tccttttgga
    28381 ggccgaccgg aagcggcggg ggaggtctgt ctcctgcgcc ttctccaggt ccttgacggc
    28441 cctgctcttc ttccccatcc tctgcaagac gagggcgcgg ttgtagtagg cgaggccgta
    28501 ctcggggtcc tcttcgatgg ccttcgtcga ggcatccacg gactcctcgt aacgctccga
    28561 ggccccgagg gctgccccca gaaacgcttt gtttctggcg agcggcttct cagctgccag
    28621 cttctcgtag agatcgaccg ccctggcgta atcgccggcg cggaaggccc tgttggcatt
    28681 gcgctccagg gaggatgtgg agaggaagct ggcgccgatt atggcgaccg cggagacggc
    28741 catccaggcc aagagcatcc gcccctgtcc aaagctctgc accaggaaca ggctgagcac
    28801 cagcaagagc agggcgagga aggggagggc tttgcgggcg agatctcccc aggccctggc
    28861 cctagccagg tcgcggttgg cggcgtagat cgtctgccag ctcacgaaga ggagcaggaa
    28921 gatcaagact atcgttatcg ctcctgggcc catgactcct cctcaggagc cgaccggcgc
    28981 cggctcgagg ctctcaagaa cctctagcgc aggggcgatc agcctatcga agtcctcgtc
    29041 ggagagtgcg gcccgtgctc cttcgacgga gacgggttcc tggagcccga tccaggactt
    29101 gcatcctccg tactcatccc ggtagggaag ctcgaccttt tcggggagca gataggcgcg
    29161 caggacgagc accgtcaaag gcttcttcgg gcgccacttg aagcggctct cggcgtactc
    29221 gggcgtccac atgtggtgcg catcgagcgc cgaaagctct gcggcgtcgg agatctcgta
    29281 ggctccctcg acctccgcga acgacgtgaa gcgcaagggt ccatcggcgg tgagatcagg
    29341 gatcccgtcc atcaggggac ggtattcctc tttgagtagt tccggcctct ggtgctcgta
    29401 accgggtaga agcaaaaagc gcgtgcgcgg cacggcgaag gctttctccc ttatgccgcc
    29461 ctttctcacg acgatcgccg tctccccacg ctcaagcgcc ctgacggcga ccgcccactc
    29521 cttgagcacg tgctgtagag tatccaagac atcacctctc gtataatgca accgttgcac
    29581 gccagattat atgacgagag cgttccggct tcgatagagg aggtacaggt gacccagcag
    29641 gagatgccgc accgcgtaga acgagattcg atgggggaag tgcaggtgcc tcgggaggcc
    29701 ctattcggcg cccagacccg ccgcgccctg gataacttcc cggtaagcga cctcagattc
    29761 cagagacgtt tcatccaggc cctcggcgcc ataaagctag agtcggcgaa cgtgaacaac
    29821 gagctgggac tgctcgacga cgaactaaag gacgccatcg tggaggcggc acaggaggtc
    29881 gtagagggac ggctcgacaa ccagttcgtg ctcgacatct tccagacagg gagcggcacc
    29941 tcgactaaca tgaacgccaa cgaggtcatc tccaaccggg ccatccagat cctggaaggc
    30001 gagcttggct ccaagagccc ggcccatccc aacgaccacg tcaaccaggg ccagtcctcc
    30061 aacgacgtta tcccgaccgc catccacctc tccgcgctcg tggccataaa ggaggacctg
    30121 ctcccgggcc tgcacaagct ccaggaggcc ctcgacgtga aggcccgcga gttcgatagt
    30181 gtggtaaaga ccgggaggac gcacctccag gacgccaccc cgatccgtct ggggcaggag
    30241 ttccgcggct acgccggcca gatcgaacgc ggcatacagc gcgtcgacaa cgccgtagaa
    30301 gacctcgccg aggtcgccct cggtggcacc gccgtcggca cgggcgtcaa cacccacccg
    30361 gatttcgctc ccagagtctg cgagagactc agcgcccgct tcggtatcga ggtgcgtgag
    30421 acggagaacc acttccaggc ccagagcgcg atggacgcgg ccgtttacgc cagcggcgcc
    30481 ttgaagaccg tcgccgttag cctgctcaag atctccaacg acatccgctg gctcggggcg
    30541 ggaccgcgcg ccaacctcgc cgagatagcc ttgccggagg ttcaacctgg ttcttcaatt
    30601 atgcccggta aggtcaatcc tgtgatcgcg gagagcgccg ccatggtcgc cgcgcaggtt
    30661 ataggcaacg acgccaccat cgccatcgcc ggacagagcg gcaacttcga gctaaacgtg
    30721 atgcttccgg ttatcgcgta caacctgatc cagagcacag agatactggg ctccgcctcg
    30781 gccaatctcg cagaccagtg cgtctccggc ctagaagcga cggagcgagg ccccgctctc
    30841 gtcgagcagg gtctcatgct ggccacggcc ctcgcccccg tggtcggcta cgacaaagcg
    30901 gcggagatct ccaaagaagc ctacaagacg ggcaagacca tccgcgaagt agcccgcgaa
    30961 agcaccgacc tatcagaaga agagctcgac aggttacttg acgcacgcaa gatgacttag
    31021 atccaggacc cgacggaagt gcttcgcgta acctcacggg cgcccttgca gtttcatggc
    31081 cagtcggcaa gcgtctcata tttgatacct gacccacctg atgttataca tcgtgtacat
    31141 acatcggagg tgtcgtggag aggacgcaaa tctaccttac aaaagaagag cgcaggactc
    31201 tggctgtcat agcggagcgc acgggacgga cgcagagcga gttgattcgt gaagcggtgg
    31261 acggcttcct cgaccgttat ggggagagaa accggctgca actgcttcgc ggtgcgcggg
    31321 gcatgtggaa agaccgcgat gacttgcccg acttcggggc gctgcgtaag gaacttgatg
    31381 agcgtggtga agctgccgaa gaagggtagt gtgtggccga gaggctgctt atagatactg
    31441 atgtgatgat cgaatacctc cgcggtcgaa gcgaagccgt agagtacctg gagggcctga
    31501 cgtcggacct ttgcatctcg gtcatctcgg tagccgaatt gtttgccgga gtcagaggcg
    31561 atgaagaaga gagatccctc aaacaattac tcctggcgtt tgtcgccctg cctgttacgg
    31621 agaagacggc ccgtctcggg ggactctacc ggagagatta tggagggagc cacggcaccg
    31681 gcctcgcaga cgccctcatc gccgcgacct ccgaagagaa tggcgcggac ctcgtaacct
    31741 tcaaccgccg ccacttcccc atggtctcca ggatcacggt gccgtacgag cggtgatcca
    31801 gagatcaacg aatccatcga aagtcgctgc gggacgcgag cgcctcgcgt ataattctcg
    31861 cgcagggaac aggagtgacg ggtatgatct ccacaccatc gacgccggtt gatgtgcctg
    31921 agccgggctc agcggaggaa ctcgagcgct tcgcggcttt gcaggcgaag ctcgagccgc
    31981 tggcgcggcg cgtgatctct gatcctcgcg ccccccagac ggtcgtggtg gtacccagcc
    32041 tcacgctcga cgtggaggag ctggcgaaga tccccggtgt acaccattac gaggaacggc
    32101 tcctttgcat gctgatgctc ttgcggttgc cccgcaccca cctcgtctac gtcacgagcc
    32161 agaacatcgc gacggccata gtagattact ttttgcacct gctgccgggc atccccttgc
    32221 gccacgcccg cagccggctg acgctcctct cctgccacga tgcctccgac gtgccgctga
    32281 cccagaagat actcgaccgt cctcgtctgg tggagcggat acgctctgcg atagcagaca
    32341 cggatgcggc gcacatgacc tgcttcaaca gcacggccct ggaacgtagc ctcgcggtcc
    32401 ggctcggcat cccgctctac ggcaacgatc cgcaactcaa ctacctcggg accaagagcg
    32461 gcagccgcga ggcgttcagg gaggccgggg tcccgctccc cgaagggttc gagcacctgc
    32521 gcgacgggga ggatgtgatc gaggccgtcg tcgagctcaa gcgccgcaaa cccggtctgc
    32581 gaagggcggc cataaagctg gatgagggtt tctcgggcga ggggaacgcc gtcttcgctt
    32641 acgaaggcgc gccagagggg agggatcttg cgcgctgggt aagggaggag ttgccggggc
    32701 gcatcaagtt cgaggcggcg ggagagacct gggggcgtta cgagaggaag ttcgaggaga
    32761 tgggcggcat cgtagagtgc tggctggagg gggaagaggt gcgctcacct tccgtgcagt
    32821 gccgaatcaa tcctcttgga caggcgaacg tcgtctccac ccacgaccag gtcctgggag
    32881 ggccttcggg ccagatcttc ctcggcagca cgttcccagc cgacgccgag tacagccgcg
    32941 acatcaagga ggccggagac agggtgggga aggtgcttaa ggaccgcggt gccctgggac
    33001 gattcgccat cgactttatc tctgtcagac gcggcgaggg ctgggagcac gcggctatcg
    33061 agatcaacct ccgcaagggc ggcaccacgc atccttacct gatactacgc ttcctgacgg
    33121 acggtactta cgacgcgaac gacggacttt actgcacgcc gacggcgaag ccctgctact
    33181 actacgcctc tgacaacctc cagagccccg tatacaaggg tctgaccccc gacgatctgg
    33241 tggacatagc cgtcgacaac ggtctccact tcgacggcgc ttcccaacag ggtgtcgttt
    33301 tccacctcat cggggcgctc tcccagtacg gtaaggtcgg caccgtctgc atcggcgaca
    33361 gccacgaaaa cgccctgaag ttctatcgag acaccgttgc cgtcttggac cgcgaggcgc
    33421 aacagtaaag gatcggaccg gtcgagccgg cggtcagact ccttcccagg tccttccgct
    33481 agaacaacca gaccctgaat agcaagtgat agacgacgta agcgaccgct gcgacgcaga
    33541 gcgcagacac caggatcacc gtgatcttgc cccaaacaga ggctcgccag acctcgtaga
    33601 cgtatcctcc gagcttccta agccacacct cgagccagtc gaggaagcgg gccgcgggga
    33661 ggagctcgcc ggccaggatc atgagcccga taaagaccgt gccccagccg gggccgggca
    33721 ggggggcgag gatcaggctg cccaaggcga taacggcgcc gagcgtgatc aggaagatcc
    33781 tccacagaat gtggccctgc tcgtcctgcc gccggcgacg gtaacggtcc ttgaaacgtt
    33841 gccctggttt gctctctcta aactcccgcc agtgctcctg caactgctcg aacataatct
    33901 aaataattcc tcatctagac ccatttgaca acccggcttg aatcctaccc gtaccggcgc
    33961 cgtgcacccg agtaacgaat gcggtatacc gagggcgtcc aaaagatacg aacggagaga
    34021 tgagagccat ggcgcttgac gataggatga acggctctgc gagggaggtc gccacgctcg
    34081 ggggtggttg tttctggtgt ctggaggcgg tctaccagga gctgcggggc gtggagaagg
    34141 tcgagtccgg gtactctggc ggacacgtgc cgaacccgac ctacagggca gtatgctcgg
    34201 agacgacagg gcacgccgag gtcgtgcagg ttacgttcga tccctcggtt gtctcgtaca
    34261 aggatattct ggaggtctat ttcactatcc atgaccctac gactttgaac cgtcagggtg
    34321 cggacgtggg gacgcagtac cggtctgtga tcttctacca cgacgacgag cagaggcgtg
    34381 tcgcggaagg ggtgatctcc gacctggaat ccgagggtat ctggagcgcc ccgatcgtga
    34441 ccgaggtaga gccgttcgac gagttctacg tcgccgagga ctaccaccag aattacttcc
    34501 gcaacaacgg tttccagcct tactgccagg tcatcatcgc gccgaaggtg gccaggtttc
    34561 gcaaggagca tctcgaccgc ctgaaggcgt aggctcgtaa cggagctcgt tcgaatcttg
    34621 aaaagagcca ttctggcgat cctgttcgct gtggttctct gttcctgtag cacggggtcg
    34681 acggcctcgg atggagcagc aggggaagac gctggcgggc cggccacgac cgtggaacaa
    34741 accgtccggg agacgaccgc cgcgccggac gaaagtaagt ccgaagagac caccgaacgg
    34801 gggtcttctg cgaccaccgc agagccggag gtgtgggaag gccccgcgag ccggaacacg
    34861 gcggtccgcc cggcggtcgg gaccaacggc atggtgagtt cggcgcaccc gcttgcgaca
    34921 cgggcggggt tggagatcat ggaggacggt ggaaacgcct tcgacgcggc ggtggcggtc
    34981 gccgcctcgc tgggcgtcgt cgagccgatg atgagcggcg tcggcgggta cggggccatc
    35041 gtcgtctaca gcgccgaaga gcgccggacc cgcttcctgg aggtcgggag ccggacaccc
    35101 gcctcgctcg acccttccgt gttccggccc ccgacgccag gctacgcgga gaatcgttgt
    35161 ggtgccaggg ctatcgccac gccgggcaac ctgaacgcct gggagacgat gtgggaggag
    35221 tacggggagg cggagtggcc gcgcctgttc cggcccgcta tcgggtacgc ggatcggggc
    35281 ttccccctcg accgggagac ggcggcctgg ctcggctccg agtacgcagc gttccccgcg
    35341 cacgcgcaag agatctacgg ccccgagggt atcccactgg ccgccggcga gagcctggtg
    35401 caggacgacc tagccgactc tttgcgcctc atctccgaag aaggctctga ggccgtctac
    35461 gagggacggc tcggctccgc gatggccgcc gccgcgcaag aggggggcgg cttcctctcg
    35521 gaagaggacc tgcgccgcaa ccgtgccgag tggcgggaga ccatcggcct ggactatcga
    35581 gatcaccgcg tagtcacggc cgccccgcct tccaccgcgt gggccgcgct agtgcggctg
    35641 ggggtgatgg gccgctacga tgcggtaccg gccggccaca acaccaccgc ctacgtccac
    35701 gcggcgacgg agatctccaa acgagtctct aaggcgaccc gccggttcgc cgccgatccc
    35761 gagaaagcac cgactccatt ggaccgcttg ctctccgacg agtacctgga gcgcgaggca
    35821 gcaagtgtgg acccgaacgc cgtcgcaccc tacgatccgc ccgcgcagtt cgacaccgcc
    35881 gcgacctgct ctcccacagg ctaccggccg accgggtatc ccgaaacgca gcgacacacc
    35941 acgcacttcg tcgtcgccga ccgggagggt aacgtcgtct ctgcgacgca gaccctgggc
    36001 aacgtcttcg ggagcaaagt catgccggag gggaccggga tctggctgaa cgacgccctc
    36061 gcgtggtcgc gcttcgagcc cgtgggcaac gtcttcgacg tgtatccggg ccgccagccc
    36121 ctctacgcgc tgtgcccgac catcatcatg cgcgacggaa agcctcgcat cgcgctcggc
    36181 acccccggag gacgaaccat ccagcagacg acgccgcaaa tgatcgccaa tatgctcgac
    36241 ttcgatatga acgtacaaca atctatctcc gcgccgcgct tcagccacat cgtccccgac
    36301 ctgctcgtaa tcgaggaggg catcccgcag tacgtcagcg acgacctctc tgccctcggc
    36361 cacgacttga tccccgccga gtatcccctg ggcaacgccc acgccctgac gatcgaatac
    36421 actcccggcg gagagacgac ccgctttacc ggcgccgccg acccgcgcgg cggcggctcc
    36481 gctgccggat actgacagcg ccgtgcataa gggcccttat gcacgacgag ccatcaacat
    36541 cccgcaacaa aaagctgacg gctgaaggct gagagctgac agcgcgagtt ggcggaaggc
    36601 atgccttccg ccaactcgct atagcccgaa gaggtcgtct tcctggctcg tctggagggc
    36661 ttcgattagt tcctcgaggc cgcccatcct taccttcatg ggtccctcgc cggccggcgg
    36721 cgggttgatg gcgacgtagt cgacctcgta cccgacggac tccagcgccc tgacgagacc
    36781 ggcgcccgag acctcgacgg gcgtgagcgc ggcgccaaag tccgtggaag ccacgaacgc
    36841 tcctgccttc acctcggagt cgaagagcgg cacggcgccg cggtcgtcca ccagtatggt
    36901 gagcggcctg gcgtcaggga ccacttcctc gaagaggagc acgtagggag gctgcggcat
    36961 ctcgttcgag atcacggctc aggaggggcg aggggccatc gtcacgagga tgaccaggtt
    37021 ttcccgcgtc tcgtttcgga cgccgtgggg gtccccggcg cgggcgataa cggcgttgcc
    37081 ctccccgaga tcctgctcct cctcgcccac tctaaagcgt cccgtgcccc gcaggacgta
    37141 gtagaccttg tccgatccct cgtgcgcgtg gaccttttgc gactggccgg ggagtaggca
    37201 gtaggcgtcg tagaagagat gaggcgagtc aaagagggcg ttcttcttca tcttctcttc
    37261 agagaacgct accgcgtctg ggatggacct gatctccatg aggtttcctc ctaacgagtc
    37321 cggaccgagg cacggctggt cactcctcga tccgtgaggc gtacgattgc ccgttacgct
    37381 tctactgtct gatccgaaaa tccttcggga acttcgtcag gttgcggcag ccatcttcgg
    37441 tcacgactac caggtcttct atgcggacgc cgcccacgtt cgggtcgtag acgccgggct
    37501 ctacggagac tacgtcgccg gggatgagct cctcgtcggc cagggagacg cggggggcct
    37561 cgtggagctc cagcccgacc ccatgccccg tgccgtggaa gaagccctcc gtgagcggct
    37621 tgccctcctc ctggtcgtga acgttcgtct tgtagccggc ctcgtgcaac acctcagcga
    37681 ccttgcggtg gacgtccttg ccgttgacgc cagaccttat catggagagg gccgcctcct
    37741 ggctctccag gactgcgtcg tacatctcct gcagcccttc gttcggttcg cctttgacga
    37801 aggtgcgggt catgtccgag tagtagcggg tcgagaggtc gacggggaag atgtcgacga
    37861 tgatggactc tcccgccttc agggggccgt ggccgcgctc gtgtgggtcg gccgcctgcg
    37921 ggccgccggc cacgatcgtg ccgtcggccg cgcagcctcg cctgaggagc tcgacgtcta
    37981 tctcggagcg cagcagctcg ctcgtgaggg tctcgccgcg ccagtcgagg acgtcgtcct
    38041 ccccgacctc ggcctcctcc aggataccga cgcagtgggc acaggcggcc tcgatggcgc
    38101 gctgggtccc ctcgatgtag gagatctcct cctccgtctt ggcgcgcctg aggccgccga
    38161 agagtttgcc gtcgggcgtg agggtgatgc cgcgggcgcg caactcgtcg gcgtagacga
    38221 tgccgaagtg ggccggcacg acgaccgggg agttcgaggc gccgatctcg tccaggaggt
    38281 tggccacggc ggcgccgtag gccctggcgc cgctcttcag ttcgcgggcg aggtcgataa
    38341 tccccaactc ctcataggag agtaactcgt ccacgggagc ctgtttctcg gcccgcccgt
    38401 attcgaggga ggagacggcc agatacctct tgccggccac acgaaggcag atcaccgcgt
    38461 ctggcgcgag aaaccccgag agatggtaag cgtcggcgtc gtgctccggc gccccgatga
    38521 ccagcaggtt ctgccctacg tcactgttca taaaggtcct tccctaaaat tggtctactc
    38581 aggcgtccca ttctactacc gtatcaggcg aaatccgtcc ccataaccgg atataatcgg
    38641 cggcgagatg cagcggcaaa acctgcacag cagggtagag gacgcgctgg acaaggttcg
    38701 gccggcgctg cgtgccgacg ggggagacgc caggatagta gcctgcgacg agaagaacgg
    38761 ctccgtcagc atacagatgc tgggggcctg caagggatgc ccgctctcgc aactcgattt
    38821 cgtctacgcc atcgaaagcc tcatcaggcg cgaggtgccc gaagtgcggg agatactcgc
    38881 ggtatagcat ctcagcacgg cggctgcgcc gccttgtcag cccgctccgc cttcgcctac
    38941 gctttcagct gtcagcttct cctgttccgg catcgctctg ctgcgtatga cgaaccgctg
    39001 ccataggcgg acgggttcta ctctcgcggt gaggctacga gacagctagc tgaaatgctg
    39061 actagccgcc gaccggaggg aggcggcggg ctgacaagcg aaggcgccag cctgagcgtg
    39121 ctgaaagctg ccgaaggcag cgtgctgaga ggctactgcc tctcctgcgg tgcagtgcgc
    39181 agggcgcccg gcggtagggc cttcctggct tcggagaggg cgtcgatagc cttcttgcgg
    39241 cttctgcgga gcctgacacg tttgaggaaa gaggcatcgg ggagggcctc gttggtgagg
    39301 tgggtaaggg tgatcatgtc gttcgcgcaa gctctgtagc cgagggcgca catctcgacc
    39361 cggacctcgt ccgagggcga ctcttcgagg gcgtgttgga tctccctggc ccgctctact
    39421 atggcgtggg agagcgcttc gggggagttg gtgtagggat cacgggctcg gtagccggcg
    39481 atcgcctggg gcgagagacg tcgtatgccg taagcaggac cgccgagcct gcggcggacg
    39541 ataaacgccg cgacgagcgt cagcgtgatt atggggacga gaaaccagag actcaagccc
    39601 gccagggacg cgactgccac gacggcgact acctcagcca ccagtatcga ggtcagatag
    39661 agcttgaaca tcgcctgcgg tacctctccg ccaccatctt cgagggacct aagtctaatt
    39721 ttctggcagc gatttggcaa gcgcggttcc gacggtctgt ataattctgc gtatgcaaaa
    39781 ggatccgtcc cccgagttcg acctgcgacg ccccttcgcg agcgcggtcg cggtgatacg
    39841 cacggtcctc ttctctccga gaagcttcta cgtaaacttc aaggtcgagg ggcctctgaa
    39901 ggaacccgcg gccttcgtgc tactcgtcgg cgcggtctgt ggcatcctcg gcgcggcggt
    39961 ggccctctcc accaaccttc tcttcggcga cctcgacgca ggggacctga gggcggcggc
    40021 gctcgacgcc ctcctcttcg cactactctc gccggtcggg gtcggcgtgg tagccggaat
    40081 ctaccttctc tcgataagga ccttcgtcgg gaaggtttcg ggcttcaggg aggtctaccg
    40141 catggcggcc tacgccgcgg ccgccctggt actcgcgtgg atacccgtgc tcggcgcttt
    40201 cgcgttctcc tacgccctta tagtccttct gggtatagcc atccagagcg tctacgggac
    40261 cacgttcttg acgaccttcg tcacgaccgt ggtgggcttc gtgcccgtcg cgtcggtcgt
    40321 gatctggctc ctcatcgccg gggtggcttc ctagggcgcc tcggaccaaa gcgcgaaggc
    40381 tgcaggcgct ctatgctacc taacgtgacc atccgccctc aggaactcgc caacgccatt
    40441 acgaacgcac tcaacccgtt cgtcatcttc acggcgctct ttgccctggt cgcctttgta
    40501 caggccggtg tctacagggg cctgctctac ctggcggtgg agtcgacagc cgcggcggtg
    40561 gtcgcggggt acgtgctctc tatgcgacga cgctcgcgag taggggactt ctggatctcc
    40621 gcccgcgccg aacgtctcgc gcccgccgtg tttctgctcg cggccttcgt ggcactgctc
    40681 gcggtcctgg tactgctcga tgccccacaa gacctctctt tgatgaccct ttcgatgggc
    40741 ctcgcgagtg cggcggtcgc cgggatcaca cttctctgga aagcgagcgc ccactgtacc
    40801 gtcgccggac acgccgcagc cgccggggtc ctgctgctcg gtcctcctgg cctcgtcttc
    40861 ctcctcgtgc tcccgctcgt actctggtca cgggtgaccc tcaaagcgca caccatatct
    40921 cagaccctcg ctggcgcggt ggtgggggcg gggtttgcgg tactctttct ggcgtaggga
    40981 agagtgtcta tgccttgcgt ccgctcgagc gtgccgttgt gcgcttctcc gatgcatgct
    41041 caccgcctga tgggttcagg cgcgtcggcg tatttcttgt acttgcccag cagtcgtcct
    41101 acggtcacct gcacggggaa aacaaaacgt tgcaccaccg ccgggatctc cgccatgtag
    41161 aactcgctcc ggtaccgccg catcaagaac gccgtctcga acggcttcgg ccggcttcct
    41221 ccgctctcgc gctgcaactc gtagatcacc gtgaggaagt actccacgtt atcggccggt
    41281 tggatgtagc ccgtgcacag gaggttctct tccccggcgt tccagaacct gtgcgcctcc
    41341 ccaggctcga aagcgatcgt ctcaccaggc tcggcatact gcggcggctc tccggaccgc
    41401 tggtagccga gtcgtccctg ttggatcgtg agtgactcct cctggtaggt gtggacgtgc
    41461 atcgggggtc cgctgccagg cttgacgaag ttctcgacct ccaggcgatc gccggcgggg
    41521 tcctggaccc gccgaacgaa ggtgattcgc tctccggccc cgttttcgat ggtatgaggg
    41581 taactgtagg tgctcatgcc cctagtgtac gaggttctgg gtcgcatagt agctgcttgt
    41641 gccgccgcgc aaggtagtaa cccgcagaaa ggagcggttc tgtacgatgc gagtctcgcg
    41701 ggtgtaggcg ggcgcacgag gggtggccta ccatccgcaa aggtggggtg agatcattgg
    41761 aaaccgggtt gctggcgaag aaggaggcgc ttcggatggc cgtttacgtc ctggtgggtg
    41821 gtggttggct cgggggctgg tgctggaagc ccgtcgcccg tcggctcagg gaggagggcc
    41881 atgacgtcta ccccgtcacc ctcaccggcc tgggtgagcg ggtacatctc gccactcctg
    41941 aggtcgacct cgagacccac atcaccgacg tggtcaacct cgtcaggttc gaggatctcc
    42001 gcgaagtggt actcctgggg cacagctacg ctggtgtcgt ggtcacgggc gcggccgacc
    42061 gcatgcccga gcgcatctcc cagctggcgt acctggacac aggaccgatc cctgacggta
    42121 cccccctcat cgattccttt ccacccgaag ttagagagca cgtcgagcgg caggtagggg
    42181 agttgggcgg cggttggcga ttcccggtgc cgccccggga agaactggct acattcggca
    42241 gtctggaagg tctggacgac accaggctag aatcgctgcg ctcacgggcc gtggaccagc
    42301 cgttcggcac ctttacgcag ccgctgcggt tggagaaccc cgccagggaa gagctgccga
    42361 aggtgggtat cctttgcagc ttctcgctcg aagaggtgcg ggcgatcatc gacagcggca
    42421 acccggtgtt ccgcgagatg gcgagcccga gttggcgatt cgtcgagcta cccacgggtc
    42481 actatcccat gttctcgagg ccagaagatc tggcgtcggt gctcctcgac ctttcatcgg
    42541 gtacgtcgac gtaaggtgat gacgattggg acggcttgag tgagagcggc ggctcctgac
    42601 ctcgagagca cagaaccgat tcggacgctc caggctggcc ttactgacga ttagattcgg
    42661 tgcggaggca gtaggaatcc gcttgggtaa acggactttc gagagtgacc ccaccgggat
    42721 tcgaacccgg gttttcgccg tgagagggcg atgtccttgg cctctagacg atggggcctc
    42781 gccgacgggc agagggagat tctacccgac gctccccgct tcgtcaaaag ggttcggagc
    42841 cgttgtctag accggtagac gctgcatctt ctcggcggcg tccctggcgc ggagcaggct
    42901 gcgttcgcct cccaaaagct gcatggtctc gaagaggccc gcgctgacgg tgcggcccgt
    42961 gacggcgaag cgcagggggt gtatcaggtg gcgcgcgccc ttctcctttt cggcggcgag
    43021 gccgcgcacc gcttcctcga tcgagtcttc cgtccattcg gggagggctc caagacgttc
    43081 gacgagctcg gggaagttct cgcggacgaa ctccttgccg aactgcttct cgaactcggc
    43141 ctcgtcgtag tcgagtgggc caccgtagaa gtagccgacc gagtcgggga tctcggtcgt
    43201 aagggcgatg cgctccttca ggagatccat gatctcagcg aggcgcgccg ggtcccgttc
    43261 gagctcatct tcggtagccg cgccagactc gacgagcatg ggagcggcga tggcggcgag
    43321 ttcctcggtg ctcttgcgtc tcagatacac ggcgttcacg gaccttagct tcttctcgtc
    43381 gaggatcgcc gggttgccgc tcacacggtc gatgcggaag cgctctgcca gctcctcggg
    43441 ggcgaagatc tcctcgtccg cggcgtagcc ggcgccgagg agcgcgaggt agttgaagag
    43501 cgcctcgggc aggtagccac gctccgcgaa gtcctcgacg ctcgccgcgc cgtggcgctt
    43561 gctgagcttc ttcttgtccg gtcccaggac ctgggggacg tgggcgaagg cggggagtgg
    43621 gttaccgagg gcctcgtgga tcaggatctg gcgcggcgtg ttggagaggt ggtcgtcgcc
    43681 gcggatgacg tggcttatct ccatctcggc gtcgtcgaag gcggcggcga agttgtaggt
    43741 cggggtgttg gaggacttca tgatcacgaa gtcctccagg ttg
//
LOCUS       DATMVF010000286        12930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_589838, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000286 DATMVF010000000
VERSION     DATMVF010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589838"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VN178_15110"
     CDS             complement(<1..648)
                     /locus_tag="VN178_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82346.1"
                     /translation="MVSPNQVMAAGLAATLASFVTSRFGVAGTLLGAALTAMIITGGS
                     AILKSYMETVSGRVRGMPTKIRSNRGQQRAGDFGGTMPGRPDLRDNFVGRMRAALDWF
                     THLPALSRRSILIKGFLAAAVAFVICMGVVWGAEKVIGNSLSCGLWAKCPNGAVPGIH
                     PAGYGGTGAGSSISGGGMNTGGVERQGQRAGLVDRVRQERNSFGDGRPIFGRDNGQ"
     gene            817..1278
                     /locus_tag="VN178_15115"
     CDS             817..1278
                     /locus_tag="VN178_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82347.1"
                     /translation="MTERDDARFTELFEVIRAYSSRGYFYQDKALQIIAGTYVFMFED
                     EEMPDVRPIVDDILGQYDYVFTTLERGNLDPLSVDAVVRVALYEDEYVEWGINRLGRI
                     LESLHRRSRADESYADFVEDAAVVIRGLENIVAGSALEEIVEAAEDANGAD"
     gene            complement(1426..4272)
                     /gene="uvrA"
                     /locus_tag="VN178_15120"
     CDS             complement(1426..4272)
                     /gene="uvrA"
                     /locus_tag="VN178_15120"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWS82348.1"
                     /translation="MAEDQIVVRGAREHNLKDITVSMPRDVMVVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDHIDGLSPAVSIDQKTTSNNPRSTVAT
                     VTEIYDYLRLLYARAGRPHCHVCGFPVASSTPQQMVEKILTLPEKTRFMVLAPVVRGR
                     KGEYGRMFKDLAESGYARVRVDGELHELPVDLGLDKNYKHDIEVVVDRLVIREGIERR
                     LTDSIETALKLAEGLVQVETVERDGAGESMLFSESFTCTNCGASIAEIQPRTFSFNSP
                     HGACDRCDGLGSRLEIDPVLVVPNEGLSVNEGAVVPWANSQTEYHRSVLGALADKYGI
                     DLDVPWRDLPEDHRFLILFGTDGERIYVSYRNRYNRRRQYMTRFDGVVGNLERRYAET
                     DSEYRREKIEEFMSHVPCPKCGGARLRPEALAVTVGGKNVYEFTELSVTDAQRFFDGV
                     EFTAREWIIGERVVKEIRERLGFLVDVGLGYLTLSRSAGTLSGGEAQRIRLASQVGSG
                     LVGVLYVLDEPSIGLHQRDNRRLLTTLEKLRDLGNTLIVVEHDEETIRSADFVIDVGP
                     GAGVHGGEVIATGSVEDIEAEERSVTGDFLAGRRRIEPPEERREPTGSIELLGAMEHN
                     LKAVDVAFPLGVFTCITGVSGSGKSTLVNEILYKALANAVSRGKHRPGRHAGLRGVED
                     VDKVIDIDQSPIGRTPRSNPATYTKVFDHIRQLFASTPEAKIRGYKPGRFSFNVKGGR
                     CEACRGHGQIRIEMHFLPDVYVPCEVCKGRRYNNETLRATYKGKSISDVLDMTVEEAC
                     EFFAPVPAISRRMETLRDVGLGYIRLGQPATTLSGGEAQRVKLASELGKRATGKTVYI
                     LDEPTTGLHFADVEKLLSILHRLVDAGNTVIVIEHNLDVIRSADHVIDLGPEGGDAGG
                     EIIATGTPEDVASVEESHTGRFLRGLMPEVVAAG"
     gene            4466..6154
                     /locus_tag="VN178_15125"
     CDS             4466..6154
                     /locus_tag="VN178_15125"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWS82349.1"
                     /translation="MILKDFSLILLQELDADCPLPGDATRRVRGLAVDEPIKGWISDD
                     QVAITTRETLDEEFLQDVASVGSPAIVWRLEGEAPAESVERAAELGVGLFVLAPEVPL
                     RRLFEIFSQDDGLLLLSHGAGRTLLESAGGETTIEELAGRISGMLGRSVVVEDPVGRL
                     IASSGSAEEDHLRATLEEQGLRASKGSSVDETRRERRDRYARLPDGYLSVPIERWRVA
                     DGELFWTPIGAGAPAGYLWLDLEGGNLRPEDVVLLYWARRVLEAELGKERIRLETELA
                     ARGDFVDDLVSGHYGSVELLLQRARYLGADLAEGALVMVVDIDDFARYLARRRPKEPA
                     IQELKRRLADAVRLQTRQLFSNFLLGPRSDNVILFLGYAEGEGPEELPEKAQLLAKGI
                     QRYVKGLLPDLTVSVGIGGFKQDPSQLPDAYSEAGVALEIGHRIHGPSSVSTFGKMGT
                     YKLLFRVLQENPVELEAFYGETLDPVVHYDSRYGTELVQTLITYLGNDASTVRTAGDL
                     FAHRHTIRYRLDRVGELTGLDVDKSEDRERLTLGIKAMQLLGRVPERPSSFSDR"
     gene            6514..7929
                     /locus_tag="VN178_15130"
     CDS             6514..7929
                     /locus_tag="VN178_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWS82350.1"
                     /translation="MRRLALLSTTVLLLVLALPGLAFAQKGPSTADLSLAMDTMWVII
                     AACLVLFMQAGFAMLEVGFSRMKNVGSVVAKILVNLAIAALLFWAFGFALAFGSGNAL
                     IGTEGWFLAVPAGQVNDIYAGLSWTQVPLSAKFLFQVAFCAVSLAIVWGTMLERTKFA
                     VYVVFAIAFAGLIYPMVGHWIWGGGWLTELGMQDFAGSTVVHLSGAMAALAGTLLLGP
                     RLGKYDDEGRPVTIPGHNMPLAVLGVLILWIGWWGFNPGSTMAAVPDIADIALTTNLA
                     AAAGVLGAMGMSYLYRRNVDVGMGGNGAIAALVAITAPCAFVAPWASIVIGFVAGIIM
                     YATLVYVDKIGVDDPLGAIAAHGMGGIWGTLSCGLFTTPALAAVGQPGLFYGGGIYQL
                     GVQALGIAAAGGFVFLASLAVFATLKATIGIRVKPEQELDGLDIHEHGVFGYPDLVAT
                     DYQNGNGKISDPLEGVPEGTA"
     gene            8055..8339
                     /locus_tag="VN178_15135"
     CDS             8055..8339
                     /locus_tag="VN178_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82351.1"
                     /translation="MESGPITQATGHNNTIVGVYEDRIELRTGWQSQNVERVGLRDVA
                     VIGIKGIVNVTLTIETNTGRVYQLNRMALPDARRIKNTFEQQKRRAGLYE"
     gene            8623..9249
                     /locus_tag="VN178_15140"
     CDS             8623..9249
                     /locus_tag="VN178_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HWS82352.1"
                     /translation="MAVVKRRPLSRRRILEAAVRFVDREGLEALSMRKLGSELGVEAM
                     SLYNHVPNKSALLDGMVEVLLGELEIPPEDEGWERRVREAYRAFRRLAHEHPNVFPLL
                     VVRPPDTMDGIWLVEKFLKTLREAGFDPQTALYAFRALSAYASGYAMAEIRGFAMEPA
                     GARDGAGTLPVDDFPHIHELDAPLREVDHDAEFEFGLDLILAGLEERL"
     gene            9281..10096
                     /locus_tag="VN178_15145"
     CDS             9281..10096
                     /locus_tag="VN178_15145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWS82353.1"
                     /translation="MRGFAGRRPFAFSVLVTLALFGLDLTSRAVFARAPVGNIEKLPQ
                     DAFEPPVGLGLILSDMRSPDTLFWALATVLALGLLVWTGWLGEAGFNRMSQWRNLHLL
                     LFPLVVCALTLSGGLFGSGPASLVSAFLTALIAAFGEEIIFRGLLWRALAPAGAVRAV
                     ILTSLLSGLLVLGMTATEGPWPEAARLTALAFCGGFTFGALRWRTTSIWPVILVHTAF
                     TFAVSIATLGAVTYPLMMLLSTAGFVAYGLYLLRNSQVRADGDLKKTAPSRAR"
     gene            complement(10109..12112)
                     /gene="uvrB"
                     /locus_tag="VN178_15150"
     CDS             complement(10109..12112)
                     /gene="uvrB"
                     /locus_tag="VN178_15150"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWS82354.1"
                     /translation="MENTTRNGFRVNSEYQPTGDQPQAIEQLASGLDAGMKAQTLLGV
                     TGSGKTHTMARVIEKAGSPALVIAHNKTLAAQLASEFAEFFPNNAVEYFVSYYDYYQP
                     EAYVPRTDTFIEKDAQINEDIDRLRHSATSSLFTRRDVIVVASVSAIYGLGSPEEYRS
                     KMVLLEQGGFYDLDDVLRDLVRIQYTRNDFQLGRGNFRVRGDVLEVHPAYQDTIYRIS
                     FFGDEVENMTEADPLTGEILREHEILTVYPASHFVTGEDRLATAVKNIEAELEERLGE
                     LEGEGKVLEAYRLKQRTQYDLEMMRELGYTSGIENYSRHLDGRPAGSTPYTLLDYFPD
                     DYVTFIDESHITLPQVRGMYNGDRSRKTTLVEFGFRLPSALDNRPLRWDEFLLKTNQM
                     VFVSATPGPYELENSGQIVEQIIRPTGLVDPEVEVRPTKNQIDDLLNEVAKRVEHGER
                     VLITTLTIKMAEDLTDYLLEHNVKARYMHSNIETLDRIQIIRGLRTGEFDVLVGINLL
                     REGLDLPEVTLVAILDADKEGFLRGERALIQTIGRAARNVNGRVIMYADKETDAMRVA
                     IGETERRREIQNSYNEQHGIEPRTITKGVSDILIAAEAKGLYKAGKKSGREAPRDPDE
                     LRTLIADLEAEMLEAAEELKFEYAAKLRDEIKDLEKQLQEVAS"
     gene            complement(12278..>12930)
                     /locus_tag="VN178_15155"
     CDS             complement(12278..>12930)
                     /locus_tag="VN178_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWS82355.1"
                     /translation="NRRTSSGACSRGGEDPTTTHTKRRRYGRSRARRRAILVLLVVVG
                     ILAILPFALRLPDAVMRIMYPLRYESAIRQASEENDLDPTFVAGVIYTESRFKPDAES
                     HRGAYGLMQLLPESARFIQRQSGIEGDYRDPEVNIRLGTWFLGYLEDRYKGEERLMLA
                     AYNSGEGNVDAWVSDEGFDIANDIPFKETRDYVDRALEAQRTYQELYGKNLQHNPG"
BASE COUNT         2170 a         4094 c         4058 g         2608 t
ORIGIN      
        1 ttggccattg tctctcccga agataggtcg tccgtctccg aacgagttcc tttcctggcg
       61 cacgcggtcg acgagacccg ccctttggcc ttgcctttcg accccccccg tattcatgcc
      121 accgcccgag atagaagagc cggcaccggt gccgccgtac ccggcggggt ggatgccagg
      181 aacggcgccg ttcggacact ttgcccacag gccgcaagag aggctgttac cgataacctt
      241 ctcggccccc cacacgacgc ccatgcagat cacgaacgcc acggcggcgg ccaggaaccc
      301 ctttatgagg atcgaacgcc ggctgagtgc cggcaggtgc gtaaaccagt cgagggctgc
      361 ccgcatcctg ccgacgaaat tgtccctgag gtcggggcgc cccggcatgg tgccgccgaa
      421 atctccggct ctctgttgcc cgcggttcga gcgtattttc gtcggcatcc cgcggacgcg
      481 gcccgagacg gtctccatat acgacttgag gatagccgac ccgccggtga tgatcatggc
      541 tgtcagggcg gcgccgagca gcgtgccggc gacgccgaag cgggaggtga cgaaagatgc
      601 caaagtagca gcgagccccg ctgccatgac ctgattcggg cttaccattg gatctgcgct
      661 cttgacactc acagacagtg aatctatcag cctgttcgcg tcctttcaaa ggccgcacga
      721 ggggttttcg tgaacagttt ctcgtggccg attctgtggc ggcgggggtc acagcgggag
      781 tagaatctcc acagagcgat cagcggaggg aagagaatga cggagcggga cgacgcgagg
      841 tttacggagc ttttcgaggt gatacgggcc tactccagca ggggctactt ttatcaggac
      901 aaggcactcc agatcatcgc cgggacctac gtgtttatgt tcgaggatga ggagatgccg
      961 gacgtccgtc ccatcgtgga cgacatcctc gggcagtacg actacgtgtt cactaccctg
     1021 gaacgcggca acctggatcc cttgagcgtg gacgccgtgg tgcgggtcgc cctctatgag
     1081 gacgagtacg tggagtgggg cataaaccgt ctcgggcgca tcctggagag cctccaccgc
     1141 cgctcgcgcg ccgacgagag ctacgccgac ttcgtcgagg acgctgcggt cgtgatccgc
     1201 ggcctggaga acatcgtggc cggctccgct ctggaggaga tagtggaggc cgccgaggat
     1261 gcgaacggag ccgactaaga gcctctaccg gcccacagac cggctgacct catcctctgc
     1321 cgcggagccg gatcggctcc tccatgaaga tccctcctgc ggcaagcagg gcggttcgcg
     1381 aacgggcggg tttgaaaccc gcccgttcta cgtgtgcaaa acgctttagc ctgcggccac
     1441 gacctcgggc atgaggccgc gtaggaagcg gcccgtgtgc gactcttcga cgctggcgac
     1501 atcttctggg gttcccgtcg cgatgatctc gccgccggcg tcaccgccct cgggtcccag
     1561 atctattacg tggtccgcgg accggatcac atccaggttg tgctcgatga cgatgacggt
     1621 gttccccgcg tctacgagcc tgtgcaggat ggagagcagt ttctctacgt ccgcgaagtg
     1681 gagcccggtc gtgggctcgt ccaggatgta gacggtcttg cccgtggccc tcttgcccag
     1741 ctcgctcgcc agcttgactc tttgtgcctc gccaccggag agcgtggtcg ccggctgccc
     1801 tagcctgatg tagccaagcc caacgtcgcg tagggtttcc atgcgccgag agatcgcggg
     1861 taccggggcg aagaactcgc aggcctcttc gaccgtcatg tcgaggacgt cggagatgga
     1921 cttgcccttg taggtggcgc gcagggtctc gttgttgtag cggcgtccct tgcagacctc
     1981 gcaggggacg tagacatcag ggaggaagtg catctctatc cggatctggc cgtgcccccg
     2041 gcacgcctcg caccgtcctc cctttacgtt gaaagagaag cgtcctggct tgtagccccg
     2101 gatcttggcc tcaggcgtgg aggcgaagag ctgccttatg tggtcgaaga ccttggtgta
     2161 ggtcgccggg ttggagcggg gcgtcctccc gatcgggctc tggtctatgt ctatgacctt
     2221 gtcgacgtcc tcgacacccc tgagtccagc gtgcctcccg ggacggtgtt ttccgcggct
     2281 gacggcgttg gccagggcct tgtagaggat ctcgttgacc agcgtgctct tccccgaccc
     2341 cgagacgccc gtgatacacg tgaagacccc gagcggaaac gcgacgtcga cggctttcag
     2401 gttgtgctcc atcgccccga gcaattctat ggaacctgtc ggctccctgc gctcctcggg
     2461 aggctcgatc ctccgccgtc cggccaggaa gtcgcccgtc accgatctct cttcggcctc
     2521 gatgtcctcg acgctgccgg tcgcgatgac ctcacccccg tgcacccccg cgccaggacc
     2581 gacatctatg acgaagtcgg ccgaacggat ggtctcttcg tcgtgctcga cgacgattag
     2641 cgtgttcccg aggtccctta gcttctccag ggtcgtcaag agacgcctgt tgtcccgctg
     2701 gtgcagcccg atggaaggct cgtcgagcac gtacaggaca ccgacgaggc cgctccctac
     2761 ctgtgaggcg agccttatcc tctgggcctc tccgcccgag agcgtccccg cagaacggct
     2821 cagggtgagg tatccgagcc caacgtcgac gaggaagccg aggcgttcgc ggatctcctt
     2881 tactacccgc tcgccgatga tccactcccg cgcggtgaac tccacgccgt cgaagaaacg
     2941 ctgcgcgtcc gtgacggaga gctctgtgaa ctcgtagacg ttcttgccgc cgacggtcac
     3001 ggccagggcc tctggcctga gcctggcacc cccgcatttg ggacacggaa cgtggctcat
     3061 gaactcttcg atcttctccc gcctgtactc cgagtccgtt tcggcgtagc gccgctccag
     3121 gttgccgacg acgccgtcga agcgggtcat gtactgcctg cggcggttgt agcggttgcg
     3181 gtaagagacg tagatgcgct cgccatcggt gccgaagagg atgagaaacc tgtgatcctc
     3241 aggcaggtcg cgccacggga cgtccaggtc gataccgtac ttgtcggcga gcgcgccgag
     3301 cacgctccta tggtactcgg tctgcgagtt ggcccacggc acaacggccc cttcgttcac
     3361 gctcagaccc tcgttcggta cgacgagaac cgggtctatc tccagacggc tccccagccc
     3421 gtcgcagcgg tcgcaggctc cgtgagggct gttgaacgag aatgtacgcg gctgtatctc
     3481 cgcgatggag gccccgcagt tcgtgcacgt gaagctctcc gagaagagca tcgactcgcc
     3541 cgcgccgtca cgctcgacgg tctcgacctg caccaggcct tcggccagct tcaaggccgt
     3601 ctcgatcgag tccgtgaggc ggcgctctat gccttccctg atcaccaacc tgtcgacgac
     3661 gacctctatg tcgtgcttgt agttcttgtc caacccgagg tcgacgggca gctcgtgcag
     3721 ctcgccgtcg acccttacgc gggcgtagcc gctctcggcc agatccttga acatcctccc
     3781 gtactcgccc ttgcggcccc gcaccacggg cgcgagcacc ataaagcgcg tcttctcggg
     3841 gagggtaagt atcttctcga ccatctgttg cggcgtcgag gaggcgacgg ggaaaccaca
     3901 gacgtggcag tgaggccgcc ctgcgcgggc gtagagcagg cgcaaataat cgtagatctc
     3961 cgtcaccgtg gcgaccgtgg agcgggggtt gttggacgtg gtcttctggt cgatagagac
     4021 cgcaggcgag agaccgtcga tgtggtcgac gtcgggttta tccatctggc cgaggaactg
     4081 cctggcgtag gcggagagac tctcgacgta gcggcgttgc ccctccgcgt agatggtgtc
     4141 gaaggctagt gaagacttgc cgctaccgga gagtccggtc acaaccacca tcacgtcccg
     4201 cggcatggag accgttatgt ccttgaggtt gtgttcgcgc gccccccgca cgacgatctg
     4261 atcttccgcg atctcttctc accccctttt tcgggacgcc tcgggcaagt atacggcccc
     4321 ctgttgggag gccaaagaag cagtttgctt ttgaccctat tctagcaccc cgaagaacca
     4381 tttccgcagc cgggtttgcg attttctgcc cctgggccca aaataataca cgccggttac
     4441 accctgctcg tgaacggagc ccgcagtgat cctgaaggac ttctccctga tccttctaca
     4501 agagttggac gcggactgtc cgctgccggg tgatgccacc aggcgggtgc gcggcctcgc
     4561 cgtcgacgag ccgatcaaag gttggatctc agacgaccag gtggccataa ccacccgcga
     4621 gactctggac gaggaattcc tgcaggacgt cgcttccgtc ggttcacccg ccatcgtctg
     4681 gcgactggaa ggcgaggcac cggcggaatc ggtcgagcgg gccgccgaac tcggggttgg
     4741 tcttttcgtg cttgcccctg aggtgcccct ccgaaggctc ttcgagatct tctcccagga
     4801 cgacgggctc ctgctgctct cgcacggagc gggccgcacg ctcctggaga gcgccggcgg
     4861 tgagaccacg atcgaagagc tggctggcag gatctcgggg atgctaggac gttccgtggt
     4921 cgtggaggac cccgtggggc gcctgatagc cagctccggg tccgcggaag aagaccacct
     4981 tcgcgcgacg ctcgaagagc agggactccg cgcctcgaag ggttcgagtg tcgacgagac
     5041 gcgtcgggag cgtcgcgacc gttacgccag gctacccgac gggtacctct ccgtacccat
     5101 cgagcgctgg cgggtcgccg acggcgagct gttctggacc cccatcggcg ccggcgcccc
     5161 ggccgggtac ctctggctcg acctggaagg cggcaacttg aggccagaag acgtggtgct
     5221 tctttactgg gccaggcgcg tcctcgaagc cgaactcggc aaggagcgca tacgcctgga
     5281 gacggaactg gctgccaggg gggacttcgt ggacgatctg gtgagcggcc attacggctc
     5341 ggtcgagctc ctcctgcaac gcgcaaggta cctcggcgcc gaccttgcgg aaggggccct
     5401 ggtcatggtc gtcgacatag acgacttcgc ccgctacctc gcacgccgta ggccgaagga
     5461 acccgccatc caggagctca agcgacgcct ggcggacgcg gtacgcttgc agacccgcca
     5521 actcttctcg aacttcctcc tcggcccgcg ctccgacaac gtcatcctct tcctggggta
     5581 tgcggaaggc gaaggtcccg aagagctgcc ggagaaagcg caactgctgg ccaaaggtat
     5641 acaacgctac gtcaagggtc tcctacccga cctcacggtc tccgtcggga tcggaggctt
     5701 caagcaagac ccctctcaac tccccgacgc gtactccgag gccggagtag cgctggagat
     5761 cgggcaccgc atccacggtc cctcaagcgt ctcgaccttc gggaagatgg ggacatacaa
     5821 actcctcttc agggtgctcc aggagaaccc cgtggagctc gaagccttct acggcgagac
     5881 cctggatccc gtcgtccact acgactcccg ctacgggacc gagctggttc agaccctgat
     5941 cacctacctc ggcaacgacg cctcgaccgt caggacggcg ggggatctct tcgcccaccg
     6001 ccacacgatc cgctaccgcc tcgaccgcgt cggcgagctg acgggcctcg acgtggacaa
     6061 gagcgaagac cgcgaacgcc tcaccctcgg cataaaagcc atgcaactcc tcggccgcgt
     6121 ccccgaaaga ccatcgagct tctcagacag atagcaattc agcacgccgg ctgcgccggc
     6181 ttgtcagcac gcttcgggcc tataaggccc tcgcttgtca gcttctcagc ttctcccgtt
     6241 ccggacctcg ccctggcacg cacgagtaac cgcttccaaa gtcggtcggg tacgacgctc
     6301 atggcgaggc tacggaacga cttgctgaaa tgctgaagtg ctgacaagtc acgaagcgcc
     6361 gtgctgactt gctgccccac cgccttgtac atccgcttga ataagagtaa gtggcggggc
     6421 acccatcttt atacatgtgg ctaaagacgc tcttgtgggc ggtgcctaga ctatgttcca
     6481 agagaatata ccctgtagag atggaggaag cttttgcgga ggctcgcact attatcgacc
     6541 acggttttgt tgttggtgct cgcgctaccc gggctggcgt tcgcgcagaa gggtccttcg
     6601 acggcggatc tctcgctggc gatggacacg atgtgggtga tcattgcggc gtgtctggtc
     6661 ctctttatgc aggcagggtt cgcgatgctg gaggtcgggt tctcccggat gaagaacgtg
     6721 gggagcgtgg tggcgaagat tctggtaaac ctcgccatcg ccgcactgtt gttctgggcc
     6781 ttcgggtttg cgctggcctt cggttcgggt aatgcgctca taggcaccga gggttggttc
     6841 ctcgcggtgc cagccggaca ggtcaatgat atctacgcag ggctgtcgtg gacgcaggtc
     6901 ccgctctcgg ccaagttcct cttccaggtc gcgttctgcg ccgtctcact ggccatcgtg
     6961 tggggcacga tgctggagcg gaccaagttc gcggtctacg tggtcttcgc catcgccttt
     7021 gcgggcttga tctaccccat ggtggggcac tggatctggg gcggaggctg gctgaccgag
     7081 ctcgggatgc aggacttcgc gggctcgacg gtcgtccacc tctcgggcgc gatggcggcg
     7141 ctcgccggta ctctgctact cggtccgcgc ctcggcaagt acgacgacga aggccgcccc
     7201 gtgaccatcc ccggccacaa catgccgctc gccgtcctcg gcgtcctgat actctggatc
     7261 ggctggtggg gcttcaaccc gggatcgacg atggccgccg ttcccgacat cgccgacatc
     7321 gccctgacta cgaacctcgc agcggccgcc ggggtcctcg gagcgatggg catgagctac
     7381 ctgtacaggc gcaacgtgga cgtcgggatg ggcggcaacg gcgccatagc ggccctcgtc
     7441 gccataaccg cgccctgcgc cttcgtcgcg ccctgggcct cgatcgttat aggcttcgtc
     7501 gccggcataa taatgtacgc caccctggtc tacgtggata agatcggggt cgacgacccg
     7561 ctcggcgcca tagcggcgca cggtatgggc ggtatctggg gcaccctctc ctgcggcctg
     7621 ttcaccaccc cggccctcgc cgccgtcggt cagcccggcc tgttctacgg cggcggcatc
     7681 taccagctcg gtgttcaggc tctcggtata gccgcggcag ggggcttcgt cttcctcgct
     7741 tcgctggccg tcttcgcgac gctcaaggcc accatcggca tcagggtcaa gcccgagcag
     7801 gagctcgacg ggctcgacat ccacgagcac ggagtcttcg gctaccccga cctcgtcgca
     7861 accgattacc agaacgggaa cggtaagatc tccgatcctc tggaaggcgt gccagaaggt
     7921 acggcgtagg gcccaaacgc gttggcaccg gccgggctat ccccggccgg tgccttttca
     7981 cgcccgccca aaatcggtag cgccgtcttg tatcctggtg cgagaggcgg tccgcaggcg
     8041 gcacggagga tggcttggaa tcaggaccca taacgcaggc cacggggcac aacaatacga
     8101 tcgtcggcgt ctacgaagac aggatcgaac tgaggacggg atggcaatcc cagaacgtcg
     8161 agcgcgtagg cctgcgcgac gtcgccgtga tcggcataaa gggcatcgtc aacgtcaccc
     8221 tgacgataga gacgaatacg ggtcgtgtct accagctgaa ccgcatggcc cttcctgacg
     8281 cgcgcaggat caagaacaca ttcgaacaac aaaaacgtag ggccggcctc tacgagtagc
     8341 ttctcagcac ggcgcttcgc gccttctcag cacgctccgg cctgcgtcct ccgctttcag
     8401 cacttcagcg tgttacgcac ctccaaaacc tgccaaagat cctccgcctg accctccgag
     8461 atcactccct tagagaccgc ccaggtaaag tccttcttcc ttaccctcat ctcctcacct
     8521 cgctcctcct gcgcgctact gcttcttaca gtgtaagctt actttgtaag tatagcgctt
     8581 atgctgtaag ctgtcaaggg tgtaagcaga aggaggtcat tgatggcggt tgtaaagcgg
     8641 cggccgttga gccggcggcg gatattagag gcggcggtac ggtttgtgga tcgggagggg
     8701 ttggaggccc tcagtatgcg gaagcttggg agtgagcttg gggtcgaggc gatgtcgctc
     8761 tacaaccacg tcccgaacaa gagcgctcta ttggatggga tggtcgaggt cctgctcggt
     8821 gagttggaga taccgcctga ggatgaaggt tgggagaggc gggtcaggga ggcataccgg
     8881 gctttccgtc ggctggcgca cgagcatccc aacgtctttc ccttgctcgt cgttcgtccg
     8941 ccggacacta tggatggcat ctggctggtc gagaagttcc tcaagacgct gagggaggcg
     9001 ggcttcgatc cccagacggc gttgtacgcc ttccgcgcgc tctccgccta cgcttccggc
     9061 tatgcgatgg ccgagatccg cggtttcgcg atggaacctg ccggcgcccg tgacggcgcc
     9121 ggcacgctcc cagtagacga cttcccgcac atccacgagt tggacgcgcc tctgcgcgaa
     9181 gtggaccacg atgcagagtt cgagttcggg ctcgacctca ttctcgccgg cctcgaggag
     9241 aggctctgac aacgagcccc gattgtacag gagcgagcag gtgaggggtt tcgcaggccg
     9301 ccgtcccttc gccttctccg tgctcgtcac tctggctctg ttcgggctgg atctcacgag
     9361 cagggccgtt tttgcgaggg ccccggtggg caacatcgag aagctgccgc aggatgcttt
     9421 cgagccgccc gtgggtctgg gtttgatcct ctccgacatg aggagccctg acaccctgtt
     9481 ctgggcactg gcgacggtgc tcgccctcgg cctgctggtg tggaccggct ggttgggcga
     9541 ggccggcttc aaccggatgt cgcagtggag gaacctgcat ctactgctct tcccgttagt
     9601 cgtctgtgcg ctgaccctct ctggtggtct cttcggatcg gggccagcgt cgctggtctc
     9661 ggcgttcctc accgcgctca tcgccgcttt cggtgaggag ataatcttcc gtggtctgct
     9721 gtggcgtgcg ctcgcgccgg cgggtgcggt gcgggccgtg atcctgacct ctttgctctc
     9781 cggcctgctc gtcctcggta tgaccgcaac tgagggaccc tggcccgaag ccgcccgcct
     9841 tacggcgctg gccttttgcg gagggttcac cttcggcgcg cttcgctggc gcaccacctc
     9901 catctggccc gtgatcctgg tccacaccgc cttcaccttc gccgtgagca tcgcgacgct
     9961 gggcgccgtc acgtaccctc tcatgatgct cctcagcacc gccggcttcg tcgcctacgg
    10021 gctctacctc ctccgcaact cccaggtgag ggcggacgga gacctgaaga aaaccgcgcc
    10081 atcccgcgcg agatagtagg gcacttgcct acgaagctac ctcttgtagc tgcttttcga
    10141 ggtctttgat ctcatcgcgg agtttggcgg cgtactcgaa tttgagctct tctgcggctt
    10201 cgagcatctc ggcttcgagg tcagcgatga gggtgcgtag ttcgtcgggg tctcgtggag
    10261 cctctcggcc gctcttcttg cctgctttgt agaggccttt ggcctcggcg gcaatgagga
    10321 tgtcggagac gcctttggtt atggtgcgtg gctcgatgcc gtgttgctcg ttgtaggagt
    10381 tctggatctc acgcctgcgt tccgtctcgc caatggctac gcgcatcgcg tcggtctctt
    10441 tgtctgcata catgatgacc cggccgttga cgttgcgggc ggcacgtccg atggtctgga
    10501 tgagggcccg ctccccgcgc aggaagccct ccttgtcggc gtcgaggata gcgacgaggg
    10561 tcacctccgg taggtccagg ccctcgcgca ggaggttgat gccgacgagc acgtcgaact
    10621 cgcccgtacg aagcccacgg atgatctgga tgcggtccag cgtctctatg ttggagtgca
    10681 tgtaacgcgc cttgacgttg tgttcgagca ggtagtccgt gaggtcctcg gccatcttta
    10741 tggtgagggt cgtgatgagc acccgctcgc cgtgttcgac gcgcttggca acctcgttga
    10801 ggaggtcgtc gatctggttc tttgtgggac gcacctcgac ctcgggatcg acgaggccgg
    10861 tcgggcggat gatctgctca actatctgcc ccgagttctc gagctcgtaa ggccccggcg
    10921 tcgccgagac gaagaccatc tggtttgtct tgagcaggaa ctcgtcccaa cggagtggcc
    10981 tgttatccag agcactcggc aacctgaagc cgaactcgac gagcgtggtc ttcctactac
    11041 ggtcaccgtt gtacatgccc ctgacctggg gcagggttat gtgcgactcg tcgataaacg
    11101 tgacgtaatc atcgggaaag tagtccagaa gcgtatacgg tgtggagccc gccggacgcc
    11161 cgtcgagatg gcgcgagtag ttctcgatac ccgaggtgta gccgagctcg cgcatcatct
    11221 ccagatcgta ctgggtgcgc tgcttgaggc ggtacgcttc caggaccttg ccctcgcctt
    11281 ccagctcgcc cagccgttcc tcgagctccg cttcgatgtt cttgacggcg gtcgcgagcc
    11341 tgtcttcgcc cgtgacgaag tgggacgcgg ggtagacggt caggatctca tgctcgcgca
    11401 ggatctcccc cgtcaggggg tcggcctcgg tcatgttctc gacctcgtcc ccgaagaagg
    11461 agatgcggta gatcgtatcc tggtacgcgg gatgcacctc cagcacgtcc ccgcgcaccc
    11521 tgaagttgcc gcggcccagc tggaagtcgt tcctcgtgta ctggatcctg acgaggtccc
    11581 tcaaaacgtc gtcgaggtcg tagaagcccc cctgctccag aaggaccatc ttcgagcggt
    11641 attcctcggg acttccgaga ccgtagatgg cagagacgct ggccaccacg atcacgtccc
    11701 tacgggtgaa cagagagctc gtagcggagt gacgcagcct atcaatgtcc tcgttgatct
    11761 gggcgtcctt ttcgataaag gtgtctgtgc gcggtacgta agcctcgggc tggtagtagt
    11821 cgtagtagct gacgaagtac tcgacggcgt tgttgggaaa gaactcagcg aactcgctgg
    11881 cgagctgcgc ggcgagcgtc ttgttgtgcg cgatgacgag cgcgggacta ccggccttct
    11941 ctatgacgcg ggccatagtg tgggttttcc cagacccggt aacgccgagc aaggtctgcg
    12001 ccttcatgcc agcgtcgagt cccgaggcga gctgctctat ggcctgcggc tggtcgccgg
    12061 tcggctggta ttcgctgttt acccggaatc cgttccgtgt cgtattctcc atacagagac
    12121 ctcgggatct ttcgtgggtt gtgaagcgtt gcgcttctgg aacatacgac tcgattatac
    12181 tgcgaatgcg gactttttct cggtcttttc ggcgctggca gcacgcgcag cgcacccatg
    12241 agagcgtggt acgcacttac cggtggattc ccgctttcta tcctgggttg tgttgtaggt
    12301 tcttgccgta gagttcctga taggttctct gcgcctcgag cgcccggtcc acgtaatcgc
    12361 gggtttcttt gaaggggata tcattcgcga tgtcgaagcc ttcatccgag acccaggcgt
    12421 ccacgtttcc ttctcccgag ttgtacgcag cgagcatgag cctctcctcg cccttgtagc
    12481 ggtcctcgag gtaaccgagg aaccaggttc ccaatcttat gttgacctcc gggtcgcggt
    12541 agtcgccctc tatgccgctc tggcgctgga tgaagcgcgc tgactccgga agcagctgca
    12601 tgagaccgta ggcccccctg tgggactcgg cgtctggctt gaagcggctc tccgtgtaga
    12661 ttacgccggc cacgaacgtg ggatccaggt cgttctcctc gctggcctga cggatggccg
    12721 attcataccg tagggggtac atgatccgca tcacggcgtc gggtagcctg agggcgaaag
    12781 gcaggatggc gaggattcca acgaccacca gcaacacgag gattgcgcgg cggcgggccc
    12841 gcgacctgcc atacctgcgt ctcttagtgt gggtcgtcgt aggatcctct cctccgcgag
    12901 aacaggcgcc cgagctcgtg cgcctgttct
//
LOCUS       DATMVF010000287         6259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_81661, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000287 DATMVF010000000
VERSION     DATMVF010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81661"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VN178_15160"
     CDS             <1..580
                     /locus_tag="VN178_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS82356.1"
                     /translation="VTEDLNLDATSVEIDERGIIQVDGYGRTAEDGVYAAGDVVGGFW
                     LAHEAGHEGIIAVEHMAGEDPMPKDRNLVPRVTFCRPEVASFGLTKAQAEEEGYEVRE
                     TKFPFRAIGKALIEGESNGFFKVVADEETDLILGMHAIGPKVTDLITEGVFAKLVEGT
                     PEEIGMTMHPHPSLSEIVGEAAMASEGHPIHF"
     gene            823..1743
                     /gene="lipA"
                     /locus_tag="VN178_15165"
     CDS             823..1743
                     /gene="lipA"
                     /locus_tag="VN178_15165"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWS82357.1"
                     /translation="MPFRQQARGVPGAHGRPEWLKARIPDGEVYRDIKETMRGLNLHT
                     VCEEARCPNIGECWNNRTATFMILGNVCTRSCGFCAVLTGKPTELDLDEPYRVADAAK
                     KMGLEHAVITSVNRDELADGGASIFAGTIRAIREEVPGCAVEVLTPDFKGDRDAVRTV
                     IDARPDTFNHNIETVPRLYFAVRPQAKYERSLEVLRYAKELNPDGLTKSGFMVGLGEV
                     EEEIHRTMRDLRDHGVDILTIGQYLRPSENHLPMSRYYTPKEFADLRKYGFSLGFKHV
                     ESGPLVRSSYHAHEQTSDARKSPAAGVASV"
     gene            1753..2415
                     /gene="lipB"
                     /locus_tag="VN178_15170"
     CDS             1753..2415
                     /gene="lipB"
                     /locus_tag="VN178_15170"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWS82358.1"
                     /translation="MTLEKQSPATTNVDVRRLPGLTPYAEAWELQKDLVRLRKNDGIP
                     DTFVLCEHPPVYTVGRAAKDAVNLGAGEEYLRTLGAEVFWSDRGGDATFHGPGQIVGY
                     PILRLKVRDTHAYLRELEDVIIRVLADYGLEGSHHPEYTGVWVGENKIAAIGVKFSSG
                     WITSHGFALNVKTDLTWFDRITPCGIKEFGITSLSQELGRDVSLAEVEDKIVDHFLRA
                     FR"
     gene            2485..2841
                     /locus_tag="VN178_15175"
     CDS             2485..2841
                     /locus_tag="VN178_15175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS82359.1"
                     /translation="MLEGVAVIWMPVQDIERAKSFYGDTLGLRITNEDGDWAEVDANG
                     LTLGLNGREPSGARGEGGPVVTFQPQAGLKETVDDLKGQGVEVPAGISEHPWGRVATF
                     KDSEGNDIQLYEAPKS"
     gene            3053..3571
                     /locus_tag="VN178_15180"
     CDS             3053..3571
                     /locus_tag="VN178_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82360.1"
                     /translation="MLITGVYGSGKSSVAEEMAAVLEERNAPYALLDLDFLAWFDTGE
                     EGGPTEHHMMLTNLAAVVGNYLMVGVRFFILAKAVRDASELDDLKVGLPMPLKVVRLT
                     VPLEEIEKRLSHDVTTGRRDDLREAAAWVAASSGIGIEDLAIPNDRPVREVAVRVLNW
                     LGWPERGSDLPG"
     gene            complement(3710..4222)
                     /locus_tag="VN178_15185"
     CDS             complement(3710..4222)
                     /locus_tag="VN178_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HWS82361.1"
                     /translation="MIAGMVLRAASFLRRKDRAFVLQYVQTTTLGLMDGSVSTLAPLF
                     ATAFATRDPRITFLVGLAAAVGAGISMGFSEGLSDDGSLTGRGSPFLRGTITGLATFV
                     GGILHTLPFLIPHVDLALYVAFGVVGLELLAISFIRYRYFDMSFLMSALQVVLGGALV
                     FASGILIGSA"
     gene            complement(4574..>6259)
                     /locus_tag="VN178_15190"
     CDS             complement(4574..>6259)
                     /locus_tag="VN178_15190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS82362.1"
                     /translation="EFLPAPWSHGNPVDVLGDADPERYAKTLETAAGDPASDGLLVVL
                     TPQDMTDPTATAELLAPYAISTRKPVLASWMGGQAVAAGESILNGAGIPTFDYPDTAA
                     RAFTNMWKYTYNLRSIYETPEPAEEESADRGRVEEIIARVRDSGRTILTEFEAKQILA
                     AYGIPTVQTEVARSPGEAVELADRLGYPAVLKLDSETITHKTDVGGVRLNLQNPDEVR
                     RAYEEMESSIKENYRPEDFGGAAVQRMVSLDGYELIVGSSLDPQFGPVLLFGSGGSLV
                     EVYRDRALALPPLTTTLARRMMERTRIFEALGGVRGRAPVDVGSLERLLVRTSQLVVE
                     QPWIKELDINPLLASPERLIALDARIVLHDPETKDEDLPRTAIRPYPKQYVSHEVLRD
                     GTPVTIRPIRPEDEPLMVRFHETLSEQSVYMRYFHMMNLDQRTAHERLTRICFIDYDL
                     EMALVAEQTDPETGEREIMGVSRLSRRGGAPDEAEFSVLVSDRFQRRGIGTLLLEHLL
                     EVGRAENLSRITAEILFDNHPMQRISKELGFHLRRDTEEMVVKADLDLYSAVR"
BASE COUNT         1229 a         1915 c         2028 g         1087 t
ORIGIN      
        1 cgtaaccgag gacctcaacc tcgacgctac cagcgtggag atcgatgagc gcggcatcat
       61 ccaggttgac gggtacggcc ggacggccga agacggcgtg tacgcggcgg gggacgtagt
      121 cgggggtttc tggctcgccc acgaggccgg gcacgagggc atcatcgccg tcgagcacat
      181 ggccggcgag gacccgatgc ccaaggacag gaacctcgtc ccgcgcgtca ccttctgtag
      241 gcccgaagtt gcctctttcg ggctcaccaa ggcgcaggcc gaggaggagg gctacgaggt
      301 cagggagacg aagttcccgt tcagggccat cggcaaggcg cttatcgaag gggagtccaa
      361 cgggttcttc aaggtcgtcg ccgacgagga gacagacctg atcctcggta tgcacgccat
      421 tgggccgaag gttacggacc tcatcaccga aggcgtcttc gccaagctcg tcgagggcac
      481 gccggaggag atcgggatga ccatgcatcc ccacccgagc ctctccgaaa tcgtcggaga
      541 agccgcaatg gcctcggaag gacacccgat tcatttctag ctgtcagcga tcagccttca
      601 gccgtcagca ggggccgctc tctgcggccc ctcgcgtgac acggcacggg tcttcctccg
      661 tgcgatacca ttagcgcagg atgcgaaaaa gataggagcg ccgtggcccg cagacagatc
      721 aacttgaagg tgttggacga tggtacgaag accttcgaag tccgccaccg ctgggaagac
      781 agaccagtgc ccccgccgcc cgacgggacg ccgacggagt acctgccttt ccggcagcag
      841 gcgaggggcg taccgggcgc ccacggtcgt cccgaatggc tcaaggcgcg catccccgac
      901 ggtgaggtct accgcgacat aaaggagacc atgcgcgggc tgaacctcca caccgtctgc
      961 gaagaggccc gctgcccgaa catcggcgag tgctggaaca accgcacggc gacgttcatg
     1021 atcctcggca acgtctgtac ccgttcgtgc ggcttctgcg ccgtgctcac gggcaagccc
     1081 accgagctag atctcgacga gccctatcgc gtcgccgacg ccgcaaagaa gatgggcctg
     1141 gagcacgccg tgatcacgag cgtgaaccgc gacgagctcg cggacggcgg ggcttcgatc
     1201 ttcgccggga cgatacgcgc gatcagggaa gaggtccccg gctgcgccgt cgaggtcctg
     1261 acccccgatt tcaagggcga tcgcgacgcg gtcaggaccg tcatagacgc ccgtcccgac
     1321 accttcaacc acaacatcga gaccgtcccc cgcctctact ttgcggtccg tccgcaggcg
     1381 aagtacgagc gctccctgga ggtgctgcgc tacgccaagg agctcaatcc cgatggcctg
     1441 accaagagcg gctttatggt cggtttgggc gaggtcgaag aagagattca tcggacgatg
     1501 cgcgacctgc gagatcacgg ggtggacatc ctgaccatcg gccagtatct caggccctcc
     1561 gagaaccacc tgccaatgag ccgctactac acgcccaaag agttcgccga cctccggaag
     1621 tacggcttct cactgggctt caagcacgtc gaaagcggcc cgctggtccg cagctcttac
     1681 catgcccacg aacagacctc ggacgccaga aagagccccg cggccggtgt cgccagcgtc
     1741 tagagcccgg gcatgaccct ggagaagcag tccccggcta ccacgaacgt cgatgtgcgt
     1801 cggctgcctg gattgacgcc ttacgcggag gcctgggagc tccagaagga tcttgtacgg
     1861 ctcaggaaga acgacgggat ccccgacacc ttcgtcctgt gcgagcaccc accggtctac
     1921 acggtgggaa gggctgcgaa ggatgccgta aacctgggcg ccggagagga gtatctgcgg
     1981 actctggggg ccgaggtctt ctggagcgac aggggagggg acgcaacctt ccacggtccg
     2041 ggccagatag tgggatatcc catcctgcgc ctgaaagtac gcgacaccca cgcctacctg
     2101 cgcgagttgg aagacgtcat catcagggtc ctggccgact acgggctcga aggttcgcac
     2161 catcccgagt acacgggtgt gtgggtgggg gagaacaaga tcgccgccat cggcgtgaag
     2221 ttttcgtcag gttggatcac ctcccacggc ttcgccctga acgtgaagac agaccttacc
     2281 tggttcgatc gcataacacc ctgcggcata aaggagttcg gcatcacgag cctgagccaa
     2341 gaattggggc gcgacgtctc cctcgccgaa gtggaagaca agatcgtcga ccactttctc
     2401 cgggcattcc gttagtttac ttactgttgc ctcggctact ggttgtaacc tgaccgcgag
     2461 cgacacatcg acaggaggca aagaatgctc gaaggtgtag cggtgatctg gatgcctgtg
     2521 caggacatag agcgggccaa aagcttctac ggggacactc tggggctccg aattacgaac
     2581 gaggacggag attgggccga ggtggacgca aacggcctca cactcggcct gaacggacgc
     2641 gagccctcgg gagcccgggg cgagggtggc cccgtcgtta ccttccagcc tcaggccggc
     2701 ctgaaggaaa ccgtagacga cctgaagggc cagggcgtcg aggtcccggc cggtatctcc
     2761 gaacacccct ggggccgcgt tgcaaccttc aaggactcag aaggcaacga catacagctc
     2821 tacgaggcac cgaagagttg agcggtcgaa ccgggtccgt atagaggcga ccacgtccgg
     2881 tctgcgcccg gggacgcacc ggagtgcttt tgcgccgttc ccatctctac tcttgtcgta
     2941 cccgcaagag cggaaatcca ctgcagagta gaggtgaggg gacgatgtcg ggccgcgttt
     3001 tgggtgccgt gtatcctgat acggtggacg agggtccgaa atagaagaag cggtgctcat
     3061 caccggcgtg tacggctcgg ggaagagctc ggtcgccgag gagatggcgg ccgtgctcga
     3121 ggagcgaaac gccccttacg cccttctcga cctcgacttc ctggcgtggt tcgacacggg
     3181 agaggagggc gggccgacgg agcaccacat gatgctgacc aacctcgccg ccgtcgtcgg
     3241 caactacctt atggtcggcg tacggttctt catcctggcc aaagcggttc gcgacgcgtc
     3301 agagctggac gacctcaaag tcgggctccc catgccgctg aaggtcgtcc gtttgacggt
     3361 cccgttggaa gagatcgaaa agcggctcag ccacgatgtg acgacgggga ggcgagacga
     3421 cctgcgcgag gcggcagcgt gggtcgctgc ttcttccgga atcggtatcg aagacctggc
     3481 gatcccgaac gaccgcccgg tacgcgaggt agcggtacga gtcctgaact ggctggggtg
     3541 gccggaacgg gggtcggatc tgccaggcta gctcccactg tggccgcgga gtttacggcg
     3601 agtgtcgagc gctcggcttc agcgaaattg taagctgagg tgctgacacg cttccacagg
     3661 aagcgtgctg atcagcgcgg ttcaaggaat cggaattgct cgaaccgtat tacgcgctgc
     3721 ctataaggat gcctgaggcg aagacgagcg caccgcctag aacgacttgc aaagccgaca
     3781 tcagaaagct catgtcgaag tagcggtagc gtataaagga gatggcgagc agctcgagac
     3841 cgacgacccc aaaagccaca tagagcgcca ggtcgacgtg tggtatgaga aaaggcaggg
     3901 tgtgcaaaat gccgccgacg aacgtggcga ggccggtgat cgtaccccgc aagaaagggc
     3961 tcccgcgccc ggttagcgac ccatcgtcag aaagaccctc cgagaagccc atgctgatcc
     4021 cagcccccac cgcggcagca agccctacca gaaacgtgat tctcgggtcc ctggtcgcaa
     4081 atgccgtcgc aaacaacggc gccagcgtag aaacagatcc gtccatcaaa ccaagagtgg
     4141 tagtctggac gtactgaagg acgaaggccc tatccttgcg ccggagaaaa ctcgccgccc
     4201 tcagtaccat cccggctatc atgcgcccat caccactctc agcacataaa aagtcaagaa
     4261 ctcgcctccc gaatacacga ctctcgacac acccattatt ctaaccaaac tctgccataa
     4321 atcaagaaca attcttgtta ttggttaatg ggagtcattt gcgatacggc ggggtagagg
     4381 aaggccttcc tctaccgcgc tggtcagcaa atcagcacgc tcaggctagc accttcgctt
     4441 gtcagcccgg cggctgcgcc gccccgtcag ccagagcttt cggtgcgcta gctgaaatgc
     4501 tgacgagtcg cctggaacag gcgacgtgct gaagtgctga aatgcgcgtc gtgcgataaa
     4561 gctcttagga gctttatcgc acggcgctat agaggtcgag atccgctttc acgaccatct
     4621 cttcggtgtc gcggcggagg tggaagccga gctccttcga gatgcgttgc atggggtggt
     4681 tgtcgaagag gatctctgca gtgatacggc ttagattctc ggcgcggccg acttcgagca
     4741 ggtgctctag gagcagggtg ccgatgcccc ttcgctggaa gcggtcgctg acgaggaccg
     4801 agaactccgc ctcatcgggg gcgccgccgc ggcgactcag gcgggacacg cccatgatct
     4861 cacgttcgcc ggtctctgga tcggtttgct cggcgaccag cgccatctcg agatcgtagt
     4921 ctatgaagca gatgcgcgtc aggcgctcgt gggcggtacg ctggtcgagg ttcatcatgt
     4981 ggaagtagcg catgtagacg ctctgttccg agagggtctc gtggaagcgc accatcaggg
     5041 gctcgtcttc gggccggatc gggcgtatcg tcacgggcgt gccgtcgcgc aacacttcgt
     5101 gggagacgta ctgtttcggg taaggacgga tcgcagttcg ggggagatct tcgtctttgg
     5161 tctcggggtc gtgcagaacg atacgggcgt cgagggctat caggcgctcc ggcgaggcga
     5221 ggagcgggtt gatgtcgagc tctttgatcc agggctgctc gacgacgagc tggctcgtgc
     5281 gaacgagcag cctctccagc gagccgacgt cgaccggcgc cctgcccctg acgccaccca
     5341 gcgcctcgaa gatacgggtc cgttccatca tccgtctggc gagcgtggtg gtcagcggcg
     5401 ggagggcgag ggccctatcc ctgtacacct cgaccagtga gccgccggac ccgaagagca
     5461 ggacaggacc gaactgcgga tccaggctgc tgccgacgat cagctcgtag ccgtcgaggc
     5521 tgaccattct ctggacggcg gcgccgccga agtcctcggg tcggtagttc tccttgatag
     5581 aggactccat ctcctcgtag gcccgcctga cctcgtcggg attctgcagg ttcaggcgca
     5641 cgccgcccac gtcggtcttg tgggttatcg tctcggagtc gagcttgagg accgctgggt
     5701 aaccgaggcg gtctgccagt tccacggcct cgccagggct acgcgccacc tccgtctgca
     5761 cggtcgggat accgtaggcg gcgaggatct gcttcgcctc gaactcggta aggatagtgc
     5821 gaccggagtc gcggacgcgg gctatgatct cctctacccg cccgcggtcc gcgctttcct
     5881 cttcggcggg ctcgggcgtt tcgtagatgc tgcgcaggtt gtacgtgtac ttccacatgt
     5941 tcgtgaaagc tctggctgcc gtatcggggt agtcgaaggt agggatgccc gcgccgttca
     6001 ggatcgactc ccccgccgcg acggcctggc cgcccatcca actcgcgagc acgggcttcc
     6061 tcgtgctgat cgcgtagggc gcgagcagtt ccgccgttgc cgtcgggtcg gtcatgtcct
     6121 gcggcgtgag gacgacgagc aacccgtcgc ttgcaggatc gcctgccgcg gtctcgagcg
     6181 tcttcgcgta gcgttcgggg tcggcgtcgc cgagcacgtc cacggggttg ccgtggctcc
     6241 agggagcggg gaggaactc
//
LOCUS       DATMVF010000288         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_81818, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000288 DATMVF010000000
VERSION     DATMVF010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81818"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VN178_15195"
     CDS             complement(<1..461)
                     /locus_tag="VN178_15195"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWS82363.1"
                     /translation="MRILLTGATGLLGGALLELLLARGHETRCLVRADSPNASRLDGQ
                     RVEILHGDAAREEDLYRALGDADALLHVAGIEYAPPVVRAAGRAGVDRVLIVGSTSAH
                     SAYAFRSGPKLKMEELVRGSGLAWTILRPSMIYGSERDKNVHRLLRFLDRSP"
     gene            complement(510..986)
                     /locus_tag="VN178_15200"
     CDS             complement(510..986)
                     /locus_tag="VN178_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82364.1"
                     /translation="MHQRGITTALLLVTIVFILIAGCGDQSGSGSQQDGGSQTEQGQN
                     APKKQDENASKKQGENAPKKKVVFGTVTFVNPKTEGFSVRPTIEEQGNKPVVFRLANE
                     TSITLDGNEAKMEDMKKGQQAQVEYVERNERNRARSVRLFETSGDSQDDQGGEKTG"
     gene            complement(1049..2098)
                     /locus_tag="VN178_15205"
     CDS             complement(1049..2098)
                     /locus_tag="VN178_15205"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWS82365.1"
                     /translation="MPYLQQSAVLDVTGDVRAYLRFEVGIVRGEVPGRPRDLLREQLA
                     SRDREISRLRTRLAQGGSDATGGGIPPGHFIWVFGVARTGSSWLGAMMGDLEDHATWY
                     EPYVGDVFGYAYYMRAGEQQRMREDYILGDPYREAWIRSLRTFVLEGAGARFPELDEN
                     GYLVVKEPNGSVGAPLLVEALPESRVILLVRDPRDVVASLLAAQRGGSWGAGEDALAD
                     TDPDEFVRQRARMYDASFGKAWVAYEAHDGRKVAIRYEDLRYDALGELQKIYSSLAIP
                     IEEEQLRRVVEKHAWENIPERQKGPNKPRRKARPGGWREDLTPEQARMVEEITTPVMN
                     EFYPGWDRGGAAPTD"
     gene            2380..3939
                     /locus_tag="VN178_15210"
     CDS             2380..3939
                     /locus_tag="VN178_15210"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HWS82366.1"
                     /translation="MFVSDSRKVVFVHIQKTGGLTVDRLLKERIPDLRRIGARHGFAS
                     LGMDELNDWDEYFKFAFVRNPWDRLVSWYSMVTTMPREGNELWRYVHDNSSTFEEFIY
                     NCTDEVEIKDGVYYSFAYNQLDYVTDEHGNLLVDFIGRLENFDKDLQEVFRRIGFQLE
                     TVPHIRAGSDHQHYSTFYSPDTELIVRERFKRDIEYFGYEFESPRRPGALFVCGSDRS
                     GTKALADYLNRHPKILVCQESSEKTEQGETALDPATFERMLEPLPKDPGDATPHNDGE
                     RLIEPRAELLANRDPSGLQWIGASNSDYLMRMESVAANNPGARFIVVYRPIEEVAESW
                     EPEDAEGRQNGDGGFGRAVRTWNRGLQGTRRFIRESLIPRVLIIDYHDFLYRRETVAP
                     LISRFLELEFDETLTAEPSDEVPPPERSGERRSLIQNQANRAAEAWILDRIEKQWVKP
                     GLYIQRTSKSALATSLDEAEARTWRLQEKVRELERDMERGRRRFKQLRSSKTWKLLER
                     ISSIRSRIAGR"
BASE COUNT          779 a         1294 c         1260 g          792 t
ORIGIN      
        1 ggcgagcggt cgaggaagcg aagcaggcgg tgcacgtttt tgtcccgctc ggagccgtag
       61 atcatcgaag gtcgaagtat cgtccacgcc agcccgctcc cgcgcacgag ttcctccatc
      121 ttgagcttcg gaccggaacg gaaggcgtag gccgagtgcg cgctcgtgct gccgacgatg
      181 agaaccctat cgacgccggc ccggccagcc gcccgtacga cggggggagc gtactcgatc
      241 cctgcgacgt gcaggagggc gtccgcgtcc ccgagcgccc ggtagagatc ctcctccctg
      301 gccgcatccc cgtggaggat ctccaccctc tgcccgtcga gcctggaagc attgggactg
      361 tctgcgcgaa cgaggcagcg cgtctcatgg ccccgggcga gcagtagttc cagtaacgcg
      421 ccccccagca agcccgtggc gcccgtgagg agtatcctca tccggcccgg ggacgtgctg
      481 gctctcgggt gcgtccctct ttatggccct cagcccgtct tctcaccgcc ctggtcgtcc
      541 tgggagtctc cgctggtctc gaaaagtctc acgctgcggg cccggttacg ttcgttcctc
      601 tccacgtact cgacctgcgc ctgctggccc ttcttcatgt cctccatctt cgcctcgttg
      661 ccgtcgagcg tgatgctggt ctcgttggcg agcctgaaaa ctaccggctt gttgccctgt
      721 tcctcgatgg tcggacgcac gctgaatccc tcggtcttgg ggttcacgaa cgtgacggtg
      781 ccgaatacta ccttcttctt gggcgcgttc tcgccctgct tcttggacgc gttctcgtcc
      841 tgcttcttcg gcgcgttttg gccctgctcc gtctgggacc cgccgtcctg ctgcgaccca
      901 ctccccgact gatcgccaca gcccgcgatg agtatgaaga cgatcgtgac caggaggagc
      961 gccgtagtta ttcctctctg gtgcatcgag tctccttgtc ctgcagcgtg ctttgcgggg
     1021 ctaaactacc agaaagggtg aaaggccgtc aatcggtggg cgctgcccca ccacggtccc
     1081 agccagggta gaactcgttc attacggggg tggtaatctc ctccaccatc ctcgcctgct
     1141 ccggcgtcag gtcctcccgc catccgcctg gcctggcctt gcggcggggc ttgttcggcc
     1201 ccttctgcct ctcggggatg ttctcccagg cgtgtttttc gaccaccctt cgtagctgct
     1261 cctcctcgat ggggatggcg agcgaagagt agatcttctg cagctcgccg agagcatcgt
     1321 acctgaggtc ctcgtacctg atcgcgacct tgcgcccatc gtgcgcctcg taagccaccc
     1381 aggccttccc gaagctcgcg tcgtacatcc tggcccgctg ccgcacgaac tcgtccgggt
     1441 cggtgtcggc cagtgcgtcc tcgcctgctc cccagctccc ccctctttgg gcggccagaa
     1501 gcgaggcgac aacgtccctg gggtcgcgga ccagcaggat cacgcggctc tcgggaaggg
     1561 cctctacgag gagcggcgcg ccaactgagc cgttcggttc ctttacgacc aggtagccgt
     1621 tctcgtccaa ctcggggaag cgcgcgccgg ccccttcgag gacgaacgtc ctgagggacc
     1681 tgatccaggc ctccctgtag gggtcgccga ggatgtagtc ctccctcatc cgctgctgct
     1741 ccccggcacg catgtaatag gcgtagccga acacgtctcc cacgtaaggc tcgtaccacg
     1801 tggcgtggtc ctccaggtct cccatcatgg cgccgagcca actactgccg gtcctcgcga
     1861 cgccgaagac ccagatgaaa tgcccgggag ggatgccacc gccggtggca tcgctgcctc
     1921 cctgggcgag gcgcgtcctc agccgggaga tctcccggtc tctggaggcg agctgctcgc
     1981 gcaggaggtc ccttggtctg ccgggaacct cgccacggac tatccctacc tcgaatctca
     2041 agtacgccct cacgtccccg gtcacatcca acaccgcgct ctgctgtaag taaggcatta
     2101 gctatctccc ccgcgtccgt gcgcggcttt tcggtcttcg tccgcaaacc gtatactacc
     2161 cagtccggtt cgagccggcc gttcgggatc cgcgatggag ggacaatatt gtccttagcc
     2221 tccgcagcgg gcaggacggc acagagcggg atgttcaggg gaggacccgt aatataccgg
     2281 ccggcggggt cgggtatccg cggctcacgc ttgaaagagc tttccttcgc aaagtagact
     2341 acgcgggtaa gtaacgtcca gagggaggat tgggcaggag tgttcgtctc cgatagccgc
     2401 aaggtggtct tcgtccacat tcagaagacg ggtggactca ctgtggacag gttattgaaa
     2461 gagcgtatcc ctgatctacg gcgcatcggg gcaaggcacg ggtttgcgag cctggggatg
     2521 gatgagctaa acgattggga tgaatacttc aagttcgcct tcgtgagaaa tccgtgggat
     2581 cgattggtct cctggtacag catggtaacg acgatgccga gagaaggcaa cgaactctgg
     2641 cgctatgtgc acgataacag ctcgaccttc gaagagttca tctacaactg cacggacgag
     2701 gtagagataa aggatggggt ctattactcg ttcgcgtaca accaactgga ttacgtgacg
     2761 gacgagcatg gcaacttgct cgttgatttc ataggccgac tggagaactt cgataaggat
     2821 ctccaggaag tattccgtag gataggcttc cagttagaga cggtcccgca cattagagct
     2881 ggctcagacc accagcatta cagtacgttc tacagccccg acaccgagtt gatcgtaaga
     2941 gagagattca agagagatat cgagtacttc ggctacgagt tcgaaagccc cagaaggcct
     3001 ggagctctct tcgtctgtgg cagcgacagg agcgggacca aggccctcgc agactacctg
     3061 aaccggcatc ccaagatcct cgtctgccag gaaagctcag agaaaaccga gcagggggag
     3121 accgccctcg atcccgccac gttcgagcgg atgctcgagc cactgccgaa ggatccgggg
     3181 gacgcaacgc cacacaacga cggggagcgt ctcatcgagc cacgcgcgga gcttttggcg
     3241 aacagggatc cgtccgggct gcagtggatc ggcgccagca actccgacta cctgatgcgt
     3301 atggaatcgg tagcggcgaa caaccccggg gcgcgcttta tagtcgtgta ccgccccatc
     3361 gaagaggtcg cggagtcgtg ggagccggaa gatgccgaag gccgccagaa tggcgacggc
     3421 ggtttcggac gcgcggtgcg tacctggaac agaggcttgc agggcacgcg gaggttcata
     3481 agagagagcc tgattccgag agtactgata atcgactacc acgacttcct ctaccggaga
     3541 gagaccgtcg cgcccctgat ctcacgtttc ctcgagctgg agttcgacga gaccctaacg
     3601 gcggagccct cggatgaagt cccgccaccc gagaggtcag gggagagacg atccctgata
     3661 cagaatcaag ccaaccgggc cgccgaggcg tggattctcg atcgcatcga aaaacaatgg
     3721 gtgaagccgg gactctacat ccagagaacc tcgaagtctg cgctcgcaac ctccctggac
     3781 gaggcggaag ccaggacctg gcgactccag gagaaggtca gagagttgga gcgcgacatg
     3841 gaacgcggac gccggagatt caaacagctt cggagctcga agacctggaa gctactagag
     3901 aggatcagca gcatccggtc caggatagcg ggcaggtaat accggcgccg cgaggagcgt
     3961 caagctggct gcctctaagg catcacgatt cgtacggcgc cccgaacgac cctaccaccc
     4021 gaaatcgacg tcgaggcatt cgtagagccg ttggttatgg ggttcgaagt aggcctccaa
     4081 ccgttgcctg gtggacgggt ccatcttctg tttgtaggtg cctga
//
LOCUS       DATMVF010000289         8407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_227801, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000289 DATMVF010000000
VERSION     DATMVF010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227801"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..343
                     /locus_tag="VN178_15215"
     CDS             77..343
                     /locus_tag="VN178_15215"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HWS82367.1"
                     /translation="MDENRLDGNAAAGLLAEIFTLEITTARAACVRCGAAGEIGAQMA
                     YVSEIGTVVRCATCDNALIRVSRQDDGPQRYWLDLKGVEYLQLG"
     gene            complement(486..653)
                     /locus_tag="VN178_15220"
     CDS             complement(486..653)
                     /locus_tag="VN178_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82368.1"
                     /translation="MPNEPQGRRVDGDGPDPTTFEEVAEPGRKPSDADIEWLSIATAD
                     PAYFIKDPDSK"
     gene            complement(716..2053)
                     /gene="aceA"
                     /locus_tag="VN178_15225"
     CDS             complement(716..2053)
                     /gene="aceA"
                     /locus_tag="VN178_15225"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HWS82369.1"
                     /translation="MEREKNGVSRIAREWEGERWEGIERPYTADDVARLRGSIRIEHT
                     LARMGAERLWRLMQERPFVRSLGALTGNQAVQQVKAGLEAIYLSGWQVAADANLAGHM
                     YPDQSLYPANSVPHVVRRINQALQRADQIHYAEGKNGIHWFAPIVADAEAGFGGALNV
                     FELMKAMVEAGAAGVHFEDQLASEKKCGHMGGKVLIPTSQAVRNLVSARLAADVLDVP
                     TVIIARTDADAASLITSDVDPSDHEFLTGERTMEGFYGVNAGMDQAISRGLSYAPYAD
                     VVWCETSEPDLEQAQQFADAIHEKHPGKLLAYNCSPSFNWKKKLSEKEISRFQEEIGD
                     MGYRFQFVTLAGFHALNYSMFDLARNYKERGMDAYSELQQAEFAAEEHGYTATKHQRE
                     VGAGYFDEIAQIVAGGAASTTALTGSTEEAQFDSPESPVTGLPGSTENEQFVK"
     gene            complement(2274..3878)
                     /gene="aceB"
                     /locus_tag="VN178_15230"
     CDS             complement(2274..3878)
                     /gene="aceB"
                     /locus_tag="VN178_15230"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HWS82370.1"
                     /translation="MTDRTRTYAEGIELTAEVPDEFAEILTPEAVAFVAKLSRQYRGR
                     VEDLLARRAERQERISAGEMPDFLPETREIREGDWEIASIPEDLQDRRVELTGPPDRK
                     MTINALNSGASCWMADFEDANCPTWHNMLESQLNMKEAIEGTITFDDPKTGKHYELNE
                     DLAVILARPRGWHLFEKHMLVDGEQVPGGLFDFGLYFFHNAQTLLDNGSGPYFYLPKL
                     ESHLEARLWNDVFETAQDELGIPRGTIKATVLIETILATFEMHEILYELREHSAGLNC
                     GRWDYIFSYIKKFREQDMLLPDRNSVTMTVPFMRAYSLLTIKTCHQRGAHAMGGMAAQ
                     IPRSDDPEWTEFANNKVREDKEREAKDGHDGTWVAHPGMVSLAKEAFDEYMPQPNQLD
                     KIPDVSPSAEDLLEKPEGNITMDGFRNNISVGVQYIAAWLAGRGAVPVFNLMEDAATA
                     EISRAQVWQWIHHQRGILEDGTEVTEDLFDKTLDEELEKIKTDIVGPERWESDEFGKA
                     AELFARISTEDEFVEFLTLPGYEYLD"
     gene            complement(3962..4171)
                     /locus_tag="VN178_15235"
     CDS             complement(3962..4171)
                     /locus_tag="VN178_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82371.1"
                     /translation="MIPVQYRDPQTEEILEHRYEEGAPAIGTRVRIGFDEYRVLYRWR
                     CVPTSCIVYVHRVVREVRKEARPAA"
     gene            4465..5424
                     /locus_tag="VN178_15240"
     CDS             4465..5424
                     /locus_tag="VN178_15240"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWS82372.1"
                     /translation="MLFRQLECFLAVARLGNLSRAAEEMYLTQPTLTARLKALEEELG
                     DPLFVRTSRGMRLTEAGREFLDYAERVVGSFEEGKRRLEELRGASGGRLRIGASPGVG
                     TYALPGLLERFAAEHPRVSISVRTGHAEAILEMTLKEEVQLGLTRAMRHPEIESLPLY
                     EDELVLIVDPGHRFTRRGTASLTEIGEEQLIFFDHDSSYFEQTHALLRNAGIRELRTM
                     EVDNIEAAKRMVEHRLGVAFLPRTAVVRSVSTGNLVLISVEENPEMSRSIVALKRRDV
                     PATGPVAAFLDVADTMGEKRDGAPLSPTLATEFENLQLIDLFT"
     gene            5631..6707
                     /locus_tag="VN178_15245"
     CDS             5631..6707
                     /locus_tag="VN178_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWS82373.1"
                     /translation="MDPKTIVVMEGDQTGQELLEEALRVLDPAVTGLELDFVRYDLSL
                     ENRRATNNEVVHQAAAAMKESGFGIKAATVTPEQPGDVGSPNAILRTGIDGTVIVRTG
                     RRIPGVNPTSGVHAPISVVRMAVDDAYGAKEWREGEGLDEVAYRTEKISRRVCRGVAE
                     FAFIQARKMRAKVFGGPKFTVSPVYEGMLKEEMDRAAENSRDVRYDPQLIDATYALLL
                     TTYGEPLVIPTLNRDGDCISDLVMQMFGSIAGAESLLISLDEEENPETVMAEAPHGTA
                     PSLEGKNVANPMAMILAGAALLTFLHEEDASRVSRAIYEATFETVLDGVKTADLGGSA
                     GTTEFTDEIIRHIRTKLEVWSALA"
     gene            6756..7223
                     /locus_tag="VN178_15250"
     CDS             6756..7223
                     /locus_tag="VN178_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HWS82374.1"
                     /translation="MSVSRALGLGAISGLRSLSGPAFVCRAASHGHLDLDGTVFAFLG
                     SPHISKALILMELGELVGDKLPATPSRTSPPPLLGRAASGALVGAVVFVSEGRRATIG
                     AALGSTAAFIAAFSGERLRALAVEKTGLPDPVVALAEDTTVLLVGLRSLKNIR"
     gene            7233..8033
                     /locus_tag="VN178_15255"
     CDS             7233..8033
                     /locus_tag="VN178_15255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS82375.1"
                     /translation="MAEERDRLRTTFDEAASLYDEVRPGYPEDLFDDIVSLSGIPAGG
                     RILEIGCGTGQATVPFARRGYRILCIELGENMAAVARRNLEGYPNAEVSTGAFEDTSS
                     QEGAFDLAISATAFHWLDPAVAYPKTARALRVGGSLALFWSVHVHSAASEGFFEAAQR
                     IYEREAREIWDHKYEGPPRSDEVPDRTCEIDSSGLFETVTRCIYRWDETYDTEGYLRV
                     LNTYSGHRSLNDDTRERLFRGIADLIDNRFGGRIVKGYLTTLYVARRR"
BASE COUNT         1483 a         2554 c         2680 g         1690 t
ORIGIN      
        1 cgacgacgct cgtcaggctc ggccacgaac cggcacgcgt caagaccgaa cgcttcggac
       61 cgactggagg atagcgatgg acgaaaacag gctcgacggc aacgccgcgg caggcttact
      121 ggccgagatc ttcaccctgg agatcaccac ggccagggcc gcctgcgtcc gttgcggcgc
      181 ggcgggtgag atcggcgccc agatggctta cgtgagcgag ataggtaccg ttgtgcgctg
      241 cgccacgtgc gacaacgccc ttatccgcgt ctcccgccag gacgacgggc cgcaacgtta
      301 ctggttggat ctcaagggcg tagagtacct gcagctcgga tagcgtattc aagcgtgcta
      361 ctgcaagtcc tttaggtact gaacacctgc ggcaaagtgg attcccgctt gcgcgggaat
      421 gacgtgtatg gggcctcggt gtgaccgcag agcccgaacg tggtctcgtt caagcgggct
      481 cggtgttact tggagtctgg atctttgatg aaataggccg ggtcggcggt ggctatggaa
      541 agccactcta tgtccgcgtc gctgggcttc cggccgggct ccgctacctc ttcgaacgtt
      601 gtgggatccg gcccatcacc gtctactctg cggccctgtg gttcgttggg catggtttcc
      661 ccctttgcta gagggagccg gagttcccgg ctccctattg cctgaagcct gtaacctact
      721 tcacgaactg ttcgttctcg gtggagccgg gcagacctgt gactgggctc tccgggctgt
      781 cgaactgggc ttcttctgtg gagccggtga gggccgtggt cgaggcggcg ccgccggcga
      841 cgatctgggc tatctcgtcg aagtagccag cgccgacctc gcgctggtgc ttggtggcgg
      901 tatagccgtg ctcctctgcg gcgaactcgg cctgctgcag ttcggagtag gcgtccatgc
      961 cccgttcttt gtagttgcgg gcgagatcga acatcgagta gttcagggcg tggaagcctg
     1021 ccagggtgac gaactggaag cggtagccca tgtccccgat ctcctcctgg aaacgtgaga
     1081 tctccttctc ggagagcttc ttcttccagt tgaaggaggg cgagcagttg tacgcgagca
     1141 acttgccggg gtgcttctcg tggatggcat cggcgaattg ctgcgcctgc tcgaggtcag
     1201 gctctgaggt ctcgcaccac accacgtcag cgtacggcgc gtaggagagg ccacgggata
     1261 tggcctgatc catccccgca ttgaccccgt agaagccctc catagtgcgc tcgccggtca
     1321 aaaactcatg gtcggacggg tccacgtcgc tcgtgatgag gcttgccgcg tcggcgtcgg
     1381 tcctcgcgat gatgacggtc gggacgtcca aaacgtcagc agccagcctg gccgagacga
     1441 ggttcctgac ggcctgcgat gtcggaatca ggaccttgcc gcccatgtgc ccgcacttct
     1501 tctcggaggc gagctggtcc tcgaagtgga cgccagcagc gccggcttcg accatggcct
     1561 tcatcaactc gaagacgttg agcgcgccac cgaaaccggc ctcggcatcg gcgactatcg
     1621 gggcgaacca atggatgccg ttcttaccct cagcgtagtg gatctggtcg gccctctgca
     1681 acgcctgatt gatcctcctg acgacgtgcg gcacgctgtt ggcagggtac agactctgat
     1741 ccggatacat gtgcccggcc aggttggcgt cggcggccac ctgccaaccg gagaggtaga
     1801 tagcctccag ccccgccttg acctgctgca cggcctgatt gccagtcaaa gccccgagtg
     1861 agcgcacgaa aggccgctct tgcatcagcc tccacagccg ctcagcgccc atccgcgcca
     1921 gggtgtgctc gatcctgatc gaaccccgca gcctggccac atcatcagcc gtgtaaggac
     1981 gctcgatccc ctcccaacgc tcgccctccc attccctggc tatccggctc accccgttct
     2041 tctcgcgctc cataaccctc tccttcactc ctcgatgccg tgtcgatact ctgctttatg
     2101 cacctatatg ctcacatatg tgcttaccgg gcgcaaggag cgcccccttt cgtgttatgg
     2161 ggcgttggta cgtggccgac gctccgtggc aggaggacgg gggccggttg ccctccagtt
     2221 cctccggccc tggtcctcgg ggtttgcatg gtggtcggga tgggtgctcc tctctagtcc
     2281 aggtactcgt aacctgggag ggtcaggaac tctacaaact cgtcctcggt cgagatacgg
     2341 gcgaagagtt cggcggcctt accgaactcg tcggattccc agcgttccgg gccgacgatg
     2401 tcggttttga tcttctccag ttcctcgtcg agggtcttgt cgaagaggtc ttccgtcacc
     2461 tcggtaccgt cttcgaggat gcctctctgg tggtggatcc actgccacac ctgggcgcgg
     2521 ctgatctctg ctgtcgccgc gtcctccatc agattgaaga cggggacggc tccgcgccct
     2581 gcgagccagg ccgcgatgta ctgcacacca acggagatgt tgttgcggaa accgtccatg
     2641 gtgatgttgc cttcgggctt ctccagaagg tcttctgcgg aggggctgac gtcgggtatc
     2701 ttgtcgagct ggttgggctg cggcatgtac tcgtcgaagg cctctttggc gagagagacc
     2761 ataccaggat gggcgaccca ggttccgtcg tggccgtcct tcgcctcgcg ctccttgtcc
     2821 tcccgcacct tgttgttggc gaactcggtc cattcaggat cgtcgctgcg tgggatctgg
     2881 gccgccatcc cccccatcgc gtgcgcgccg cgctggtggc aggtcttgat ggtgagcaac
     2941 gagtaggcgc gcatgaacgg gacggtcatg gtcaccgagt tgcggtcggg gaggagcatg
     3001 tcctgctcgc ggaacttctt gatgtaggag aagatgtagt cccaccggcc gcagttgagg
     3061 cctgcggagt gctcgcggag ctcgtagagg atctcgtgca tctcgaacgt ggcgagtatc
     3121 gtctcgatca ggaccgtagc cttgatggtg ccccttggta tgcctagctc gtcctgggcc
     3181 gtctcgaaga cgtcgttcca aagcctggcc tcgaggtggc tctccaactt gggcaggtag
     3241 aagtaaggac ctgagccgtt atccagcagc gtctgcgcgt tgtggaagaa gtacaggccg
     3301 aagtcgaaga ggccgcctgg gacttgttct ccgtcaacga gcatgtgctt ctcgaagagg
     3361 tgccatccgc gcggccttgc aaggatcaca gccaagtctt cattcaactc gtagtgcttg
     3421 cccgtcttcg gatcgtcgaa ggtgatggtg ccctcgatcg cttctttcat gttgagctgg
     3481 ctctcaagca tgttgtgcca ggtcgggcag ttggcgtctt cgaagtccgc catccagcag
     3541 ctcgccccgg agttcagggc gttgatcgtc atcttccggt cgggtggccc cgtcagctcc
     3601 acgcgccggt cctgaaggtc ctcggggatg gaggctatct cccagtcgcc ctccctgatc
     3661 tcccgcgtct ccggcaggaa gtcgggcatc tcgccggcgc tgatcctctc ctgcctctcg
     3721 gcccgcctgg cgaggaggtc ttccaccctc cccctgtact gtcgcgagag cttcgccacg
     3781 aaagcgaccg cctcgggcgt gagtatttcg gcgaactcgt cggggacctc ggccgtaagc
     3841 tcgataccct cggcgtaggt gcgcgtccta tccgtcatga cctccggctc ctctctacag
     3901 cttccaaaga gaggctactc tagcaagcct cgccccagaa cgctcggaac cgaaggcctg
     3961 ctcaggccgc aggcctggcc tccttacgga cctccctgac caccctgtgc acgtagacga
     4021 tgcacgaggt cggcacgcaa cgccacctgt agaggacccg gtactcgtcg aaacctatcc
     4081 tcacccgcgt cccgatggcc ggcgcaccct cctcgtagcg gtgctccaaa atctcctccg
     4141 tctgcgggtc cctgtactgt accggaatca tagctggctc ctctcaacct cgccgtcgtg
     4201 aaagcaagat accaacccac tcggtattat tccaattgtt tgcatgtatc gcaggtataa
     4261 gttttactta tacctgcgag ctagtcagca cgcttcggcc tgcggcctac gcttgtcagc
     4321 acggcggcgt tgccgccttg tcagcatgtc agcgagatac aaaactgaca ggctgaaatg
     4381 ctgaaagcgg aggcgaagct ggagcgtgct gacaaggcgc cgtcaagcgc cgtgctgacc
     4441 agctggcgac cgaagggaga cagcgtgcta ttcagacagt tggaatgttt tctggcggta
     4501 gcgcggctgg ggaacttgag ccgggctgct gaggagatgt accttacgca gcctactttg
     4561 acggcacgtc tcaaggcgct cgaggaggag cttggggacc cgctctttgt aaggacgagt
     4621 cgtgggatgc ggctcacgga ggctggcagg gaattcttgg attatgcgga gagggtcgtt
     4681 ggcagcttcg aggagggcaa gcggcggttg gaggagctca ggggggcatc ggggggacgg
     4741 ctcaggatcg gggcttcccc cggagttggg acgtacgcgt tgccggggct attagagagg
     4801 ttcgcggcgg agcatccgag ggtttcgatc tctgtcagga cggggcatgc cgaggcgatc
     4861 ctggagatga cgctcaagga ggaagtgcag ctcggtctga cgcgggcgat gcgccacccc
     4921 gagatcgaga gcctgccgct ctacgaggac gagttggtgc tgatcgtaga tccagggcac
     4981 cgattcacca ggagggggac tgcgagtctg accgagatcg gtgaggagca actcatcttc
     5041 ttcgaccacg actcgagcta cttcgagcag acgcacgccc tgctcaggaa cgccggcata
     5101 agggagctca ggactatgga ggtcgacaac atcgaggccg ccaagcggat ggtcgagcac
     5161 aggttggggg tcgcgtttct gccccgcacg gccgtggtca ggtccgtctc taccggaaac
     5221 ctcgtgctca tatccgtaga ggagaatccg gagatgagcc gttcgatcgt tgcgctcaag
     5281 cgccgggacg tcccggcgac aggccccgtt gccgccttcc tggacgtcgc cgacacgatg
     5341 ggcgagaagc gggatggcgc cccgctctca cccaccctgg ctaccgaatt cgagaacttg
     5401 cagctgatag acctgttcac ttagagcggt tcgtatccgg gcggttcggg aaccgtgttc
     5461 cgggtgagtt tgaaacccgc tcctacaggt tgccgttcgg attcatgtgc acatctttgc
     5521 gccctaccct tgttgtctga tgtcttggtg ttgcggagca cgaatgtgtg tagaggtgga
     5581 tcgtatactc taggggatac tcaaagagac tattcacaga ggtgcgacga gtggacccga
     5641 agacgatcgt tgtaatggag ggtgaccaga cgggccagga gctgctcgaa gaagccttac
     5701 gggtgctcga ccctgcggtg accgggctcg agttggattt cgtgcgctac gacctgtcgc
     5761 tggagaaccg acgtgccacg aacaatgagg tcgtgcacca ggctgcggcg gcgatgaaag
     5821 agtctggatt cggcatcaag gcggcgaccg ttacgccgga gcaaccgggg gacgtgggct
     5881 cgcccaatgc gatcctgaga acggggatag acgggaccgt tatcgtgcgt acggggaggc
     5941 gcatccctgg tgtcaacccg acgagcggcg tgcacgcccc gatctccgtg gtccgtatgg
     6001 ccgtcgatga cgcttacggg gcgaaggagt ggcgcgaagg cgagggcctc gacgaggtcg
     6061 cctacaggac cgagaagatc tcgcgccggg tgtgccgcgg cgtagcggag ttcgccttta
     6121 tccaggcgcg caagatgcgg gccaaggtct tcggcggccc gaagttcacc gtctcccccg
     6181 tctacgaggg gatgctcaaa gaagagatgg accgcgccgc ggagaacagc cgcgacgtcc
     6241 gctacgaccc ccagctcata gatgctacct acgcactgct attgaccact tacggcgagc
     6301 cgctcgtcat ccccaccctc aaccgtgacg gtgactgcat ctcggacctc gtgatgcaga
     6361 tgttcggctc catagcgggg gccgaatcgc tcctgatctc gctcgacgag gaggagaatc
     6421 cagagacggt catggccgag gcgccgcacg gcacggctcc ttcgttagag ggtaagaacg
     6481 tagccaaccc gatggccatg atcctggcgg gagccgcgct cctcaccttc ctccacgaag
     6541 aggacgcgag ccgggtctca cgcgccatct acgaggcgac cttcgagacc gtcctagacg
     6601 gtgtaaagac cgccgatctc ggtggttcgg cgggcacgac cgagtttacc gatgagatca
     6661 tacgccacat caggaccaag ctggaggtgt ggagtgctct ggcgtaagac cgttgggggc
     6721 gggtttgaaa ctcgcccgta cgggagcgcg ctcgcgtgag cgtttctaga gccctcggcc
     6781 tcggcgccat ctcaggcctg cgctccctct ccggtccggc ctttgtgtgc cgcgccgcga
     6841 gccatggtca tctggatcta gatggtacgg tcttcgcctt tctcggctca ccgcacatct
     6901 ccaaggcgct catcctcatg gagcttgggg agctcgtcgg ggacaagtta cccgccacgc
     6961 cgagccgaac ctccccacct cccctgctcg ggcgcgctgc ctcaggagcc ctggtggggg
     7021 cagtggtctt cgtctcggaa ggccgtcgcg ctacgatcgg ggcggcgctt ggttctacgg
     7081 cggccttcat cgctgccttc tcaggagaac ggctccgcgc gctggcagtc gagaagaccg
     7141 gtctccccga ccccgttgtc gcactcgctg aggacacgac tgtcttgctc gtcggtctcc
     7201 gctcgctgaa gaacatccgt tagcgcaggc ccatggccga agagagggac aggctcagga
     7261 cgaccttcga tgaggcggcg tcgctctacg acgaggttcg tcccggctat ccggaagacc
     7321 tcttcgacga catcgtctcg ctctcaggaa taccggccgg aggaaggata ctcgagatcg
     7381 ggtgcggcac gggccaggcc acggtgccat tcgcccgccg cggctaccgc atcctgtgca
     7441 tcgagctcgg cgagaacatg gccgcggtcg cccgccgcaa tctggaaggg tatccgaacg
     7501 cggaagtgag caccggagcc ttcgaagata cctcttcgca ggaaggagct ttcgatctgg
     7561 cgatttcggc gaccgccttt cactggctag atcccgccgt ggcctatccc aagacagcca
     7621 gggcgctcag agtcggaggt tcgctcgccc tcttctggag cgttcacgtc cacagcgccg
     7681 cgagcgaagg cttcttcgag gctgcgcaga gaatctacga gcgtgaggcg agggagatct
     7741 gggatcacaa atatgaaggg cctcccaggt ccgacgaggt gccggacagg acatgcgaga
     7801 tcgatagctc tggcctcttc gagacggtga cccgctgcat ctatcgctgg gatgagacgt
     7861 acgacaccga aggctacctg cgcgttctga acacgtactc cgggcacagg agcctaaacg
     7921 acgacacccg tgagcgtctc ttccgcggca tcgccgacct tatcgacaat cgtttcggag
     7981 ggcgcatcgt caagggctat ctgactactc tgtacgtggc ccgcagaagg tagaggtcat
     8041 cccacttaat ggcggccatg aatgtgccga ggatcaagat gtgtcccgta agagaggaag
     8101 ttgcgtaagg ctcgcaggtg gtgcaacgag ccgccgcagc atccggttgg gaggatacga
     8161 ctattcttgc ccgggtgttt acttcctcac cgtttgtgct gcacaacgcg ggctcttgtt
     8221 cggtggggtc tcggaaggca gagtggtcga gaacgcgaac gcaagcatcg tgcgcgactg
     8281 ttggtcagac ttaccaaacc attatccgca cgtctcattg gacgcgttcg tggtgatgcc
     8341 gaaccatata cacgggatta ttgttttgcg tgacgccgat ggggcggggt tgcaagatcg
     8401 ggccggt
//
LOCUS       DATMVF010000290        11297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_33917, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000290 DATMVF010000000
VERSION     DATMVF010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33917"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VN178_15260"
     CDS             complement(<1..502)
                     /locus_tag="VN178_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWS82376.1"
                     /translation="MHLQHQSNPYRTHSAGELRKDNAGERVRLAGWVHRRRDHGGLIF
                     IDLRDRWGITQVTFHPERDGIFSSAEQLRPEWSVSVEGEVVRRPEGNENPDLPTGAIE
                     VEATGLQILNPSETPPFEIERDRPVDETLRLKYRYLDLRRGRMRDKILLRHRVVKHMR
                     DYLDDRD"
     gene            645..1685
                     /locus_tag="VN178_15265"
     CDS             645..1685
                     /locus_tag="VN178_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82377.1"
                     /translation="MDCAGSPENVGERSILGKGDGGAHAGSVVYLAFLLGCLFVFAGC
                     GGDESQAPVTPTQSPETSTPAGTPTENTVVGEGQAESTQDEAFRLNTAQPVPPNFEEA
                     YGRRARLVVQFYKLDQDSLSYPQGLSVDRKVNQAMMRLSSKYPTIEFFSYGIANPGPV
                     GEEQSLQPGEYGTLAAQLGVGITPFIAMLAPSGEEYVITNLFEGYVPKPVLSQALFDL
                     AAVQVEDNTSDANVTLEQVQTTEEGGGIEYFTVQNRGTTSVNLQGFTVRVLDPETAQV
                     TPGAPGVTIDSDIRVERGQSVSVGRVPDIVDDEGERVAGTFEGGTSLDLAPGDQVALL
                     DPGGAVASTITV"
     gene            1946..3106
                     /locus_tag="VN178_15270"
     CDS             1946..3106
                     /locus_tag="VN178_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWS82378.1"
                     /translation="MTEPVYLDNAATTPLDPRVLESMLPHLGGRRGNPSSLHAFGGLA
                     RERLDEARDSVASLIGASPGEIVFTSGGTESDDLAVLGLARSAGPEKRHAVVSCVDHA
                     AVREAARRLKYEGFEVSWIGVDADGLVDPDEFAASLRPDTALAAVVYANNEVGTIEPL
                     PQLAEISSERDVPFHADAVQAAGRVPIDVSETPVSTLALSGHKLYGPQGVGALYVREG
                     VSLEPIIYGGGQEGGLRSGTQNVAGVVGFGTAARLAREEMRGRVTHEMALRDRVMSGV
                     ETIPGVFVNGHRERRLSNNVHLTVSGVEAESLVLFLDALGYAIGSGSACSSGGHKASP
                     VLLAMGLTEREAFSVVRITVGKDNTPEEIDGFLGAFSTAVEQLREISPLYAG"
     gene            3120..4580
                     /gene="mnmA"
                     /locus_tag="VN178_15275"
     CDS             3120..4580
                     /gene="mnmA"
                     /locus_tag="VN178_15275"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWS82379.1"
                     /translation="MSDRYRPQVIDHLVRPRNAGELSDPSGRGESGDASCGDVACFTV
                     GISDNVVEHVRYKVYGCAACIAAGSALAELVEGKHLLDGARVSKADLEGALGGPLPAG
                     KEHALTLVLDALHKAFEDHWNRKAGDMLGANGGRGAGGRSVVAAMSGGVDSAVTALLL
                     KETGYDVATVTFRLHDGERGSRSCCSPDTVLFARDTAHRMGLPHFTLNLKELFQRRVM
                     QDFVGSYNEGKTPNPCVACNAHVKFHASSFLADELGFHHVATGHYARVEEGPTLARPV
                     DESKDQTYVLWPVPRELLSRTIFPLGDYRKTDVRAIAEERGLAVAYTPESQDICFIPD
                     GDYRGFIRKKVAAKPGDVVDGEGRVLGRHAGVVDFTVGQRRGIGVSAPTPLYVSEVRP
                     RSGQVVVGCRQDLEVREVRVRDLNWFLGPEQAACVQVRYNGSPVPCTVEETADGGWVA
                     YLDEPVMGVAAGQSAVFYTGDGERVVGGGVVARRSS"
     gene            4655..5035
                     /locus_tag="VN178_15280"
     CDS             4655..5035
                     /locus_tag="VN178_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HWS82380.1"
                     /translation="MELFVELMKGLLFAGIAVAFFVLAWMFLRLASIFSTTEQTIKDV
                     SGQVVPLLGKTHITVDNINSQLTQLDEAVGDVAGMTGELDQTTAVITRGVRGGVIKVS
                     SATAGISRGLSTFLGGERRPEREE"
     gene            5038..5346
                     /locus_tag="VN178_15285"
     CDS             5038..5346
                     /locus_tag="VN178_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82381.1"
                     /translation="MAGWGRVVAGVVIGVAGTIYATNEDVRKNLPKAARDLPDNVRRR
                     YRSAVVAAREASTKRREEILHDLEEHGGGNHAARGVKPDAAPVNESDEDASTDMPEQR
                     "
     gene            5362..5649
                     /locus_tag="VN178_15290"
     CDS             5362..5649
                     /locus_tag="VN178_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82382.1"
                     /translation="MTEDYPQEYAYLRLPPHEELRSCAGLVLAGMAARARVGVGGLEE
                     AVEVLEGLHADDAPTRFRFSLAEDGVLAEVEEPATGESTETSWRTVVELVS"
     gene            5646..6458
                     /locus_tag="VN178_15295"
     CDS             5646..6458
                     /locus_tag="VN178_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HWS82383.1"
                     /translation="MSRRYRRPDKARTRRLLVRYHKQGDENARERVIQEQLPLAEFLA
                     RKFAGRGEPLDDLIQVASVGLIKAVDRFDVDRKIEFSTYATPNILGEIKRYFRDKGWA
                     MRVPRGLQELRQSAKEVIRNETVRTGRSPTLQELAETLDADVEGVAEALTLGRAYNTA
                     SLDAPVSQDEPEGDTMMDFQADDASPIEGLEDRILLQEAIEGLRDQQQEILKLRFNEG
                     KTQTEIADHIGVSQMHVSRLLRRALEDLRVELEYLENERETRELSPDGAHAE"
     gene            6448..9081
                     /gene="alaS"
                     /locus_tag="VN178_15300"
     CDS             6448..9081
                     /gene="alaS"
                     /locus_tag="VN178_15300"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWS82384.1"
                     /translation="MQSNEIRRRFLGFFGERGHRLYPSASLIPHNDPTVLLTTGGVQQ
                     FIPYFLGQERPPSPRAMSAQKCFRTPDIEDVGDWHHLTFFEMLGNFSFGDYFKKEAIG
                     WAWEFVTEELGIDPERLWVTIFEGDEDAPEDVEAKEHWMGVGVPEERIFGLPKSENWW
                     GPPGETGPCGPCSEIYYDHGEEFARGDPHEDPKYGPGGEEGDPRFLEIWNLVFNQYEQ
                     LKDKSFVPLDKTGIDTGMGLERTTAAVQNVLYVYDTDLYASIFERASEYTGVSLGDSE
                     ITDRALRIVADHARGMAFLIADGVRPGNQRREYVLRRIIRRAMLQAHSRLGMSPGQLA
                     SLAEVVVDYMGGFYEELRTAREDIRRIVTGEATRFVEIYESGMSLLASEIERMRGGHF
                     PGEVAFMLHDTYGFPVEVTREVLAERGLSLDEAGFEAAMGAQRERAREAMQGYERVVA
                     AFRDQEIRSRFVGYEREQVETRIVAVEPVPDAAGEVYVVLADNPFYATGGGQVADGGW
                     ITSEDGQLEVFDAIPAGDYQVLRARTERGAFEVGDAVTASINRVRRQQIEANHTATHI
                     LHWALRAVLGQDVVQAGSYVGPDRLRFDYRYGGRVTDEEIGRVQELSLQKITENQPVR
                     YFTTTLEEARNLGAIMLFGEKYADLVRVVEVDGFSRELCGGTHVRGTAEIGAFKILSN
                     RKHGADLYRIEVITGREALYYLTGATEKAEEVSESLKVEIEDLPGAVGRLREEVEASR
                     EAAREQALKRGLEEVGDLVEGAQQVNGTRVVTGQVAAADVRGLRQISDDVKNRLGAPS
                     AVVLAAALGGKAVLVANFHPEVSRRVEAGEIVREVSGVLGGGGGGGATMAQAGGGNLE
                     AIPDALARVREILDQRLADPE"
     gene            9085..9492
                     /gene="ruvX"
                     /locus_tag="VN178_15305"
     CDS             9085..9492
                     /gene="ruvX"
                     /locus_tag="VN178_15305"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWS82385.1"
                     /translation="MSPADRGRVAALDLGEVRTGVAVSDPGGVIASPLEVIPSEDLPA
                     YLSRLVDEEDVYEVVVGVPKTLRGEVGYQARKVLARLDALKTEFPGVRFVEWDERFTT
                     RLAGRGRTKGAMRVDHLAAAAMLQEYLRAREGL"
     gene            9489..10598
                     /gene="mltG"
                     /locus_tag="VN178_15310"
     CDS             9489..10598
                     /gene="mltG"
                     /locus_tag="VN178_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWS82386.1"
                     /translation="MRRYDEGGRRDQEEIDARLDALQKSGSTRGRRKRRPKAGSAVLG
                     VVLVLGILGVIYFIYSAATGGEDGSKEAARVQVVKGDTLSDVAAKLEEAGAIESAFVF
                     ELQARYEGYGTEIRTGEYTFRPGQASGEILHELTVGRPAPTITVTIPEGLTIEETAGT
                     VAADSSVSATAFEEAARRTDYGYAFLDDPDVKTTEGYLFPAKYDFEKGVTARQIVDRL
                     LEQYLLETQDIDFAEAKERLHLTEDQVVTVASLIEKEAASPGEKPLIASVIYNRMRKD
                     TPLQIDATIQYALKRPKANLSLADLKVDSPYNTYEKEGLPPGPICSPGRESLLAALNP
                     ANTNYLYYVLEANGEKHFFTKSYEDFLREKEKAER"
     gene            10814..>11297
                     /locus_tag="VN178_15315"
     CDS             10814..>11297
                     /locus_tag="VN178_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWS82387.1"
                     /translation="MGEQAIKGRIGGGITRRRFLGTLTAGTALVALSGPLACEPGSRT
                     RASAAPQGPGRARTFRSRPDLRPPAIKVNANLPGTAPGHIFVSPKKGPDEEAPTQDAP
                     LIVDASGEPVWFHPLQDAEADAFTFEVQTYKGERVLTWWEGLHTGYGQGHYVILDSSY
                     R"
BASE COUNT         2150 a         3430 c         3824 g         1893 t
ORIGIN      
        1 agtcccgatc gtcgaggtag tcgcgcatgt gcttgacgac gcggtggcgg agcaggatct
       61 tgtctcgcat cctcccccgg cgcaggtcga ggtagcggta cttaaggcgg agcgtctcgt
      121 cgacgggccg gtcgcgttcg atctcgaaag gcggtgtctc ggaagggttc agaatctgta
      181 gtcccgtggc ctcgacctct atcgctcccg tgggcagatc ggggttctcg ttgccctctg
      241 gtcggcgcac cacctcgccc tccacggaga cggaccactc aggcctgagt tgctcggccg
      301 aggagaagat cccgtcccgt tcggggtgga atgtgacctg cgtaatgccc cagcggtcgc
      361 gcaggtctat gaagattagg cctccgtggt ccctacgcct gtgtacccag cccgcgagcc
      421 ttactctctc cccggcgttg tccttgcgta gctcgcctgc gctgtgggtt ctgtacggat
      481 tactctggtg ctgaaggtgc aatttcgttc tcctggcggt cgtttgcagg ccggtccacg
      541 aatgttagcc gaaaacgctg cggaccgaga ccgatggtct atccggatgc atattgcggt
      601 gttttcccca ccgtgctaag ttagcgggaa tcggatcgga ggccgtggat tgcgccggat
      661 ctccggagaa tgtaggggag aggtctatcc tgggtaaggg cgatggagga gcacacgccg
      721 gaagcgtagt atatctggcg ttccttctcg gttgcctgtt cgtgttcgcg ggatgcggag
      781 gggacgagag ccaggccccc gtaacgccca cccagagtcc cgagacgtcg acccccgcag
      841 gcacgcccac cgaaaacacc gtggtgggag aaggtcaggc cgagagcacg caggacgagg
      901 ctttcaggct caacacggcc caacccgtgc cgcccaactt cgaggaagcc tacgggcgca
      961 gggccaggct cgtggtccag ttctacaaac tggaccagga ctcgctctcc tatccacagg
     1021 gtctctccgt ggaccgcaag gtcaaccagg ccatgatgcg cctgagctcc aagtacccga
     1081 ccatcgagtt cttctcctac ggcatcgcga atcccgggcc cgtgggcgaa gagcagagcc
     1141 tgcagcctgg cgaatacggt acgctggccg cgcaactcgg ggtcgggatc acgccgttca
     1201 tcgcgatgct tgcgccgagt ggcgaagagt atgtgatcac caacctcttc gaaggatacg
     1261 tgccaaagcc cgtgctcagc caggcgctct tcgatctcgc ggcggtacag gttgaggaca
     1321 acacgagcga tgccaacgtg accctagaac aggttcagac caccgaagaa ggcggcggaa
     1381 tcgagtactt caccgtgcaa aatcgtggga ccacgtccgt gaacctgcag ggttttaccg
     1441 tgcgggtcct cgaccccgag accgcgcagg ttacgcccgg tgcgcctggc gtcacgatcg
     1501 acagcgatat cagggtagag cggggtcaga gtgtctccgt aggccgcgtt cccgacatag
     1561 tagacgacga gggcgagcgc gtcgcgggca cgttcgaggg gggcacatcg ctcgatctgg
     1621 cgccgggaga ccaggtcgcg ctcctcgacc ctggtggcgc cgtcgcctcc accatcaccg
     1681 tctgatggca cgtcgtgcca tcagacggtg agcatttcag catctcagca cgctcaggct
     1741 tttagccttc gcttgtcagc acggcgcttc gcgccttgtc agcgtgtcag acgaggctcg
     1801 cacacgctgc aagctgaaat gctgacaagt gcccgacagg gcacgtgctg aaatgctgac
     1861 cagcgcgggt tgaggaaggc ccgagccttc ctcaacccgc taaacggctg acattttggc
     1921 cctttaagat aaactaggct cgatcatgac cgagcctgtt tacctggaca atgccgccac
     1981 cacgccgctc gatccgcggg tcctggaatc gatgcttcct catctcggtg gccgacgcgg
     2041 caatccttct tcgctgcacg cgttcggagg cttggcgcgt gaacggctgg atgaagcccg
     2101 agactccgta gcctccctga tcggggcttc gcccggggag atagtgttta ccagcggcgg
     2161 gaccgaatcg gatgacctgg cggtcctcgg tctggcgaga tccgccggcc ccgaaaagcg
     2221 gcacgcggtc gtctcttgcg tcgatcacgc ggccgtgcga gaggcggcga gacgcctaaa
     2281 gtacgaaggt ttcgaggtgt cctggatcgg ggtcgacgcc gacggcctcg tcgaccctga
     2341 cgagttcgcc gcctcgctgc gccccgacac cgcgcttgcc gcagtcgtgt acgcgaacaa
     2401 cgaggtcggc accatcgagc ccttgcccca gctcgccgag atctcctctg aaagggatgt
     2461 tcctttccac gccgacgccg tccaggccgc cggaagggtg cctatcgacg tctcggagac
     2521 gcccgtctcg acgctggccc tctcggggca caagctctac ggccctcagg gcgtcggtgc
     2581 cctctacgtg cgtgagggcg tctcgctcga accgatcatc tacggaggag ggcaagaggg
     2641 gggactgagg agcggcaccc agaacgtcgc aggggtcgtc gggtttggca cggctgcgag
     2701 gttggcccgc gaggagatgc gcggtcgcgt cacccacgag atggcgctgc gcgaccgcgt
     2761 aatgtccgga gtagagacca tcccaggtgt gttcgtaaac gggcatcgcg agcggagatt
     2821 gtccaacaac gtccacctca cggtatccgg cgtggaagcc gagagcctgg tgctctttct
     2881 cgacgcactc ggctatgcta taggaagtgg ctcggcctgt tcctcgggtg ggcacaaggc
     2941 ctcaccggtc ctgctcgcga tggggttgac cgagcgagaa gcgttctccg tcgtcaggat
     3001 cacggtcggc aaagacaaca ccccggagga gatcgatggc ttcctggggg cgttctcgac
     3061 cgcggtcgag caactgagag agatctcccc gctgtacgcg ggttgaaggc gggttagacg
     3121 tgtccgatcg ttacagaccg caggtcatag accacctcgt ccggccgcgc aacgccggtg
     3181 agttatccga tccttctggc aggggggagt ctggcgacgc ctcctgcggc gacgtcgcct
     3241 gctttacggt cggtatctcg gacaacgtcg tggaacatgt ccgatacaag gtctacggct
     3301 gtgcggcgtg catcgccgcc ggctcggccc ttgcggaact ggtcgaaggc aaacacctcc
     3361 tcgacggggc ccgcgtttcg aaggccgatc tggagggcgc gctcggcgga cccctccccg
     3421 ccggcaagga gcatgcgctc acgctcgttt tggacgcttt gcataaggcc ttcgaggacc
     3481 actggaaccg caaggcgggc gatatgctcg gcgcgaacgg aggacgggga gccggcggca
     3541 ggagcgttgt ggccgcgatg agcgggggag tcgactcggc ggtcacggcc ctgcttctca
     3601 aagaaaccgg ctacgacgtc gcaaccgtga ctttccggct gcacgatggc gagaggggca
     3661 gccgctcgtg ctgctcgccg gatacggtac tcttcgcccg cgacacggcg cacaggatgg
     3721 gactgccgca cttcacgctc aacctcaagg agctcttcca gaggcgcgtc atgcaggact
     3781 tcgttggctc ctacaacgaa ggcaagacgc cgaacccctg cgtcgcttgc aacgcccacg
     3841 taaagtttca cgcgtcgtca ttcctcgcgg acgagctcgg attccaccac gtcgctacgg
     3901 ggcactacgc cagggtggag gaaggcccca cgcttgcgcg ccccgtagac gagagcaagg
     3961 atcagaccta cgtcctgtgg cccgtgccgc gggagctgct ttcgcgtacg atcttccccc
     4021 tcggcgatta ccgaaagacg gacgtccgcg ccatcgccga ggagcgtgga ctcgccgtcg
     4081 cctacacccc ggagagccag gatatctgct ttatccctga tggagactac cgcggcttta
     4141 tccgcaagaa ggtggcagcg aagccgggag acgtcgtcga cggcgaaggg cgcgtactcg
     4201 gccgtcacgc cggcgtggtc gactttactg tcggacagcg gcgcgggata ggcgtctcgg
     4261 cgccgacgcc gctgtacgtc agcgaggtaa gaccgcgatc cgggcaggtg gtcgtggggt
     4321 gcaggcagga cctcgaggtg cgcgaggtca gggtgaggga tctcaactgg ttcctcggtc
     4381 ccgagcaggc cgcttgcgtt caggtccgct acaacggctc gccggtccct tgtacggtgg
     4441 aggagaccgc tgacggaggg tgggtggcct acctcgacga gccggtcatg ggtgttgctg
     4501 cgggccagtc cgccgtgttc tacaccgggg acggggagcg ggtcgtcggg ggtggcgtcg
     4561 tcgcccgccg tagctcttga gagcggtacc ttttgcgcgt aatcacagct ataatccggg
     4621 tttacaggga ccccgtaggg aggtgctgta gaggttggaa ttgttcgtag agctgatgaa
     4681 agggttgctc ttcgcgggga tagccgtcgc gttcttcgtc ctcgcgtgga tgttcttgag
     4741 gctggcgagt atcttctcga ccaccgagca gacgatcaaa gacgtctcgg ggcaggtcgt
     4801 tccgctgctt gggaagacgc acatcacggt ggacaacata aacagccagc tcacgcagct
     4861 cgacgaggcc gtcggcgacg tggcaggcat gacgggcgaa ctcgaccaga ccacggccgt
     4921 gatcacgcgc ggcgttaggg gcggtgtgat caaagtctcc tccgcgacgg cgggtatctc
     4981 gcgcgggctc agcactttcc tcggcggcga aagacgacct gagagggagg agtagcgatg
     5041 gctggttggg gtagggtcgt ggccggcgtc gtgatcgggg tggcaggcac gatctacgcg
     5101 acgaacgagg acgtgcgcaa gaatttgccc aaagcggcgc gcgacctccc cgacaacgtc
     5161 cgtcgccgct acaggagcgc ggtagtggcg gcacgcgagg cctccacgaa gcggcgggag
     5221 gagatcctgc acgacctcga ggaacacggt ggcgggaacc acgccgcgcg cggggtgaag
     5281 cccgatgctg ccccggtgaa cgagtcggac gaagatgcat cgacagatat gcccgagcaa
     5341 cgctagggaa gggaccctga cttgaccgaa gattatcctc aggaatacgc ttacctgagg
     5401 ctcccgcccc acgaagagtt gcgttcctgt gccggtctcg tgctggcagg gatggcggcc
     5461 cgggccaggg taggagtggg aggattggag gaggccgtgg aagtactgga gggtcttcac
     5521 gccgatgacg ctccgacccg ctttcgcttc tcactggccg aggatggggt tctcgccgag
     5581 gtcgaagaac cggccaccgg ggagagcaca gagacgagct ggcgcacagt agtcgagctg
     5641 gtgtcgtgag tcgacggtat cgcaggccgg acaaagcgcg cacgcggcgt ctgctcgtgc
     5701 gctatcacaa gcagggcgac gagaacgccc gcgagcgggt cattcaggaa caactccccc
     5761 tggccgagtt tttggcccgc aagttcgcgg gccgcggaga acccctggac gatctgatcc
     5821 aggtcgcctc cgtcggcctg ataaaggcgg tggaccgctt cgacgttgac aggaagatcg
     5881 agttctcgac ctacgcgacc cctaacatcc tcggggagat caaacgctat ttccgtgaca
     5941 agggctgggc gatgcgggtc ccgcgcggcc tgcaggagct gcgccagtcg gccaaggaag
     6001 tcatccgcaa cgagaccgtc agaactggac gctctcccac cctgcaggag ctcgccgaga
     6061 cgctggacgc cgacgtcgag ggcgtcgcgg aggcgttgac cctcgggcgg gcttacaaca
     6121 cggccagcct ggatgcgccc gtaagccagg acgagcctga aggggacacg atgatggact
     6181 tccaggccga cgatgcgtcc ccgatagaag ggctggagga caggatcctg ctccaggagg
     6241 ccatcgaggg cctcagggac cagcagcaag agatcttgaa gctccgcttc aacgagggca
     6301 agacgcagac cgagatcgca gaccacatag gcgtcagcca gatgcacgtc tcccgcctct
     6361 tgaggcgggc actggaagac ctgcgggtcg aactggagta cctggagaac gagcgcgaga
     6421 ccagagagtt gtcgccggat ggggcgcatg cagagtaacg agatacgacg ccggtttttg
     6481 gggtttttcg gggagcgggg ccacaggctc tacccgagcg catccctgat cccccacaac
     6541 gaccctacgg tcctcctcac cacgggcggg gtccagcaat tcatccccta ctttctcggt
     6601 caggagaggc caccttcacc gcgggcgatg agcgcgcaga agtgtttccg cacccccgac
     6661 atcgaggacg tcggggactg gcatcatctc accttcttcg agatgctcgg aaacttctcc
     6721 ttcggggact acttcaagaa ggaagccata gggtgggcct gggagttcgt gacggaggag
     6781 ctgggcatcg atcccgagcg cctgtgggta acgatcttcg agggcgacga agacgccccc
     6841 gaagacgtgg aggcgaagga acattggatg ggcgtcggcg tgcccgaaga gaggatcttc
     6901 ggcctcccca agagcgagaa ctggtggggg ccgccgggag agaccgggcc gtgtggcccg
     6961 tgctcggaga tctactacga ccacggtgaa gagttcgcca ggggcgaccc tcacgaggac
     7021 ccgaagtacg gtcccggcgg agaggagggg gatccacgct tcctcgagat ctggaacctc
     7081 gtcttcaacc agtacgagca gttgaaagac aagtcattcg tgcccctgga caagaccggt
     7141 atcgacaccg gcatgggatt ggaacggacc accgccgcgg tacagaacgt tctctacgtc
     7201 tacgacaccg acctctacgc atccatattc gaaagggcga gcgagtacac gggcgtctcc
     7261 ctgggcgact cggagatcac ggatcgcgca ctgcgcatcg tggccgacca cgcgcgcggc
     7321 atggccttcc tcatcgccga cggtgtcagg cccggaaacc agcgcaggga gtacgtactt
     7381 cgccgcataa tacgcagggc gatgttacag gcgcattccc gcctcggcat gagccctggc
     7441 cagctcgcct ctctggccga ggtcgtggtc gactacatgg gcggcttcta cgaggagtta
     7501 cggacggcgc gtgaggacat acgtcgcatc gtcaccggcg aggccacgag gttcgtcgag
     7561 atctacgaat cggggatgtc gcttctggca tccgagatag agcggatgcg gggcgggcac
     7621 tttcctggtg aggtggcgtt catgttgcac gacacgtacg gctttcccgt ggaggtgacg
     7681 cgggaggtgc tcgccgagag gggtctgtcg ctcgatgagg cgggtttcga ggctgcgatg
     7741 ggcgcgcaac gggagcgggc cagggaggcc atgcagggct acgaacgggt cgtcgcggcg
     7801 ttcagggatc aggagataag gagccgcttc gtcggctacg agcgcgagca ggtagagacg
     7861 aggatagtcg ccgtggagcc cgtgcccgac gcggctgggg aagtctacgt cgtactggcc
     7921 gacaaccctt tctacgccac cggcggcggg caggtggcgg acgggggttg gatcacctcg
     7981 gaggacggtc agctcgaggt cttcgatgcc atcccggcgg gtgactatca ggtgctcagg
     8041 gccaggacag aacgcggcgc gttcgaggtt ggggatgcgg taacggcctc tataaacagg
     8101 gtgcgccgcc agcagataga agcgaaccac acggcgacgc acatcctgca ctgggcgctg
     8161 agggcggtcc tcggacagga cgtggtgcag gcgggcagct acgtcgggcc cgacaggttg
     8221 cgcttcgact accgttacgg agggagggtc acagacgaag agatcgggcg cgtccaggag
     8281 ctctctctgc agaagataac cgagaaccag ccggtcaggt actttacgac cacgctcgag
     8341 gaggctcgca acctcggtgc gataatgctc ttcggggaga agtatgccga cctggtgcgt
     8401 gtggtcgagg tcgatggatt ctcgcgcgag ctgtgcggcg ggacgcacgt gcgcggcacc
     8461 gccgagatcg gggctttcaa gatactttcc aacaggaagc acggcgccga cctctaccgc
     8521 atcgaggtca taacgggtag ggaggcgctc tactacctaa ccggggcgac cgagaaggca
     8581 gaagaggtct ccgagtcgtt gaaggtcgag atcgaggacc tccccggggc cgtaggacgg
     8641 ctgcgcgagg aggtagaagc gtctcgcgag gcggccaggg agcaggcact aaagcggggt
     8701 ctcgaggagg tcggcgacct cgtcgaaggc gcccagcagg tcaacggtac gagggtggta
     8761 acgggccagg tcgccgccgc cgacgtcagg gggctgcgcc agatctccga cgacgtcaag
     8821 aatcgtctcg gcgctccctc cgcggtggtg ctggccgctg ctctcggagg caaggccgtg
     8881 ctcgtcgcca actttcaccc cgaagtctcc cgcagggtcg aagccgggga gatagtgcgg
     8941 gaagtctcgg gcgtactcgg gggtggcggc ggtggcggcg cgacgatggc ccaggccggc
     9001 ggcggaaacc tcgaggccat ccccgacgcc ctcgccaggg tgcgcgagat cctcgaccaa
     9061 cgcctcgcgg acccggagta agtcctgtcg ccagccgacc gcgggcgagt cgccgccctc
     9121 gacctcggcg aggtgcgcac cggcgttgcc gtttcggacc cgggaggtgt gatcgccagc
     9181 ccgctcgaag tgatacctag tgaggatctc cccgcttacc tgagccggct cgtcgacgag
     9241 gaggatgtct acgaggtcgt cgtaggcgtg ccgaaaacgc tcagggggga ggtcggctac
     9301 caggcccgca aagttttggc tagactagac gccttgaaga cagagttccc tggggtgcgc
     9361 ttcgtggagt gggacgagcg attcacgacc cgcctggccg gccgtggcag aaccaagggg
     9421 gctatgaggg tggatcacct ggctgccgca gcgatgctgc aggagtacct cagggcaagg
     9481 gagggtcttt gaggcgctac gatgagggag ggcgacgaga ccaggaggag atcgatgcca
     9541 ggctcgacgc gctccagaag tccggttcca caaggggccg ccgcaaaagg cgcccgaaag
     9601 ccggatcggc cgtgctgggc gttgttctcg tgctcggcat tctgggagtt atctacttca
     9661 tttactccgc ggcaacagga ggcgaggacg ggtcaaagga agcggcgagg gtgcaggtcg
     9721 tcaaaggcga tacgctctcg gacgtcgccg ccaaactcga agaggccggg gccatagaga
     9781 gcgccttcgt cttcgagctg caggccaggt acgaaggcta cggcacggag ataaggaccg
     9841 gagagtatac cttccgaccg ggccaggcta gcggggagat cctccatgaa ctgaccgtcg
     9901 gacggcccgc gccaacgatc acggttacga tccccgaggg actcacgata gaagagaccg
     9961 cagggaccgt cgccgcagac agcagtgtaa gcgcgaccgc gttcgaggag gccgcgcgta
    10021 gaaccgacta tgggtacgcc tttctcgacg atcccgacgt caaaacgaca gaagggtacc
    10081 tctttcctgc caagtacgat ttcgagaagg gcgttaccgc caggcagata gtggacaggc
    10141 tcctcgagca gtatctacta gagacccagg atatcgactt cgccgaggcg aaagaacgtc
    10201 tccaccttac ggaggatcag gtcgtaaccg tggcctccct gatcgagaaa gaggccgcca
    10261 gccccgggga gaagcccctg atagcgtcgg tgatctacaa ccgcatgcgc aaggacacgc
    10321 cgctccagat cgacgccacg atccagtatg cgcttaagag accaaaggcg aacctctcgc
    10381 tggcggacct gaaggtagac tcgccttaca acacctacga gaaagagggg ctgcccccgg
    10441 gacccatatg cagccctgga agagagtcac tcctggccgc gctgaacccc gcgaatacca
    10501 actacctcta ctacgtcctc gaggccaacg gcgagaagca tttctttacc aagagctacg
    10561 aagatttcct ccgggagaaa gagaaggcag aacggtgacc ttgcaaacct gcggcttttc
    10621 gtggaagatc ccgctctgca gggccgatcc gcgcaggagt gagttcgctc gtgatgcgtt
    10681 cgagcccgcc gaaactcctt gacggagcac atgtggcgca cgagaagagg ccttcgtggc
    10741 ttccgctcgt gcgtagggga gaaggcccag ggcctacccc ctttcgatgc tggctagaga
    10801 cgagagggcg aatttgggcg agcaggcgat caaagggaga attggaggag ggatcacacg
    10861 ccggaggttt ctggggaccc tcacggcagg aaccgccctg gtcgcgctct ccggccccct
    10921 cgcatgcgag cccggctcgc gcacccgggc gtccgccgcg ccccaggggc ccggacgggc
    10981 caggactttc cgctcgcgcc ccgatctccg tccacccgcc atcaaagtca acgcgaatct
    11041 gcccggcacg gcccctggcc acatcttcgt ctcgccgaaa aagggcccgg atgaggaagc
    11101 tcccacccag gacgcaccgc taatcgtcga cgccagcggc gagcctgtct ggttccatcc
    11161 gctgcaggat gccgaggccg atgctttcac cttcgaggtc cagacctaca agggggagag
    11221 ggtgctcacc tggtgggagg gactccatac cggctacggc cagggccact acgtcatcct
    11281 cgatagctcc taccgcc
//
LOCUS       DATMVF010000291         7283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_639623, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000291 DATMVF010000000
VERSION     DATMVF010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639623"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1559
                     /locus_tag="VN178_15320"
     CDS             129..1559
                     /locus_tag="VN178_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWS82388.1"
                     /translation="MGDPKGFLKIGRKPTPKRKIEERVRDYRYVYEPMPDDELRGQAS
                     RCMDCGVPFCNTGCPLGNLIPDWNDLVYRENWKQAIDRLHQTNNFPEFTGMLCPAPCE
                     AACVLAINDKPVTIKEIELSIINRAFEEGWVVPRPTPPEKRTGKKVAIVGSGPAGLAA
                     AQQLNWAGHTAVVFEKDDRIGGLLRYGIPDYKIEKWVVDRRIAIMKEEGVAFRTNAHV
                     GENPTTEDLKSEFDAIVLAIGALQGRDLDVPGRELGGIHLAMDYLVQQNRRVAGLPTN
                     TEVEISAAGKNVVILGGGDTSADCLGNAHREGCASVKVLTHGPRPPDTPDHLTWPDWP
                     FILHTYPAHEEGGERGFSVAVKGFSGSNSHVERMHAIETERSSDGKTIPREGTDFEIE
                     ADLVLLAIGFTGPVRDRFLRDMDLGYTERGAIHSENGFGTKEPGIFVAGDARLGADLI
                     VTAIAEGRKAAKQVDEFLMGRSLLPG"
     gene            1662..2915
                     /locus_tag="VN178_15325"
     CDS             1662..2915
                     /locus_tag="VN178_15325"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWS82389.1"
                     /translation="MGLIEFSVEKARELLLAAQGLGTVPKRATKDDVREIIRRMGVLQ
                     IDSISVVARSPYLVLWSRLGSYEPRWLDELLAEGALFEYWSHAACFIPIEDYGLYRRL
                     MIEGGEKTRTWMRAHHEEIEHVMEHISGKGPVRAAEFARTDGKAGGWWEWKPEKRALE
                     HLFASGELMISRRENFHRVYDLRERVLASALPDWEDARAPNEQEVRRALALKAVRALG
                     VAPARWVPDYFRTPKKGVAGLLEELADEGELVRARIEGEPAYVHPDNARLAEKILSGN
                     LQPAFTTLLSPFDPVVWDRARALELFDFEYKIEVYTPAARRRYGYYSLPILHNGALVG
                     RLDAKAHRKQGTFEVKAIHLEPNTPVNEDLISGLSGALRDCAGWHGTPEVVVRRSEPS
                     DLTETLRLALDEETVPPTPGSTKTT"
     gene            3032..3499
                     /locus_tag="VN178_15330"
     CDS             3032..3499
                     /locus_tag="VN178_15330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS82390.1"
                     /translation="MDTGVNPIPEGFHTVTPYMTIKDAASAIEFYKEAFGAREIFRWA
                     DSDGRVRHAEIVIGDSPIMLTDEAPDFGMNSPQSLGGSPVHVFLYVEDVDELFGRAIA
                     AGATELMPVENSEDGDRRGGLTDPFGHVWYIGTHIEGISREELQKRYDAFADR"
     gene            complement(3759..4520)
                     /locus_tag="VN178_15335"
     CDS             complement(3759..4520)
                     /locus_tag="VN178_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWS82391.1"
                     /translation="MVLEYSGGGDPQRTMALLWGIGKKPTRGPKPGLSVGRIVGAAVE
                     VADSEGLGALSMRRVADRLGIGTMSLYTYVPGKAELIDVMLDTVLGEVAESGSVELGW
                     RAGLEKIARDNWALYHRHPWMLQVAATSRPPLGPNVIAKYDRELRVVDGIGLTEVEMD
                     SVLALVLEHVQGAARRSVEVAEAEKRTGKTDDEWWLANEPLLEKVFDPDLYPTASRVG
                     PAAGDAYGSAYDPEYAFEFGLQRILDGVEALVRSR"
     gene            4605..4949
                     /locus_tag="VN178_15340"
     CDS             4605..4949
                     /locus_tag="VN178_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWS82392.1"
                     /translation="MKLNHLNLTVTDVPAACDFLERYFDLRRMGGNAGMAFLSDDDGF
                     VLTLMKARRATGNAYPGNFHIGFFVEGGEIVDEINRRLKEDGFDVAPPEQHHAYTFYV
                     EAPGGFTVELGS"
     gene            4980..5954
                     /locus_tag="VN178_15345"
     CDS             4980..5954
                     /locus_tag="VN178_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWS82393.1"
                     /translation="MESARFAVFAEGLEKRYGKVKALDGLDLRIPGGKVCGLLGPNGA
                     GKTTAVRILSTLLRPEGGRAEVAGFDVVRQAGQVRCSIGLAGQQAAVDEILTGRQNLE
                     TFGRLYHLGVREARERADDLLERFGLADAAGKQAKNYSGGMRRRLDLAASFILSPPVL
                     FLDEPTTGLDPRGRNEVWEAVRSLVAGGTTVLLTTQYLDEADQLADRICVIDAGRAIA
                     EGTPEDLKSRVGGDRIDVVVRSAGELPVAARVVGRVCGAEPEIDQESRRVSAPVMDRL
                     SALTGTVRALDDADVDAEDIGLRRPTLDDVFLRLTGHRAGEKEEEVAA"
     gene            5951..6898
                     /locus_tag="VN178_15350"
     CDS             5951..6898
                     /locus_tag="VN178_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWS82394.1"
                     /translation="MTHPTPAIPRSVPDRLRWAIADAWTVARRDLIHWFRQPFGIIVT
                     LLFPVMLVLMFGYLFGGAISLPGGNAAGGGKYREFLMPGMFAMTMAFGLEATMTSVAT
                     DAARGVTDRFRSMPMAPSAVVLGRSIADMMSSALGLSVMIACGLLIGWRWHESIGQAL
                     LAVGLLLLLRFSLLWVGIFLGLMVKSPEAVAAVQILIWPVLFLSNTFVAPETMPGWLG
                     AISEWNPLSATVAATRELFGNPGWGGETWISQNSLLLAVLWPTVIFAVFFPPLRQTLP
                     ASEPLTSDQGTGALVTLPLCSSRCGSRASTRGIHRWQRA"
     gene            complement(6822..>7283)
                     /locus_tag="VN178_15355"
     CDS             complement(6822..>7283)
                     /locus_tag="VN178_15355"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWS82395.1"
                     /translation="EILSDLFAGDDSPAVEIIRSTPGKLAGWATYDLPSVPTWYRGSM
                     IIIGDAAHATAPSSGQGASMAIEDAVVLARCLRDLPDTGQAFAVYERLRRARVERIVA
                     HGARTSNSKAAGPVARVLRDLMMPLILKRVASGGSLAWMHDYHIDWNTKVA"
BASE COUNT         1371 a         2316 c         2333 g         1263 t
ORIGIN      
        1 gagcgaccgg cgcggcgcgt cctcgacgac tgggacgagg tcttgcccag attcgtcaag
       61 gtaatacccc acgacctcaa gcgggtcttg caggaaaggc aggaggccga gctcgaggtc
      121 gcaagctaat gggagatccc aagggattcc tcaagatcgg acgcaagccg accccgaaac
      181 ggaagataga ggagcgcgtc cgggactacc gctacgtcta cgagccgatg ccggatgatg
      241 agctcagggg ccaggcctcg cgctgcatgg actgcggcgt gcctttctgc aacacgggct
      301 gccccctagg gaatctgatc cccgactgga acgacctcgt ctaccgcgag aactggaagc
      361 aggccataga ccgcctgcac cagaccaaca acttccccga gttcacaggg atgctctgcc
      421 cggccccgtg tgaggcggca tgcgtcctcg ccatcaacga caagcccgtt accataaagg
      481 agatagagct ctccattatc aatagggcct tcgaggaagg atgggtcgtc cccaggccga
      541 caccgccgga gaagcgtacg ggcaagaagg tcgcgatcgt cgggagcggg cctgccggcc
      601 tcgccgcagc tcagcagctc aactgggccg ggcacaccgc agtcgtgttc gagaaggacg
      661 ataggatcgg cggcctcctc aggtacggaa tccccgacta caagatcgaa aagtgggtcg
      721 tcgaccgccg catcgccatc atgaaagagg agggcgtcgc gttccgcacc aacgcacacg
      781 tcggcgagaa cccgaccaca gaggacctaa agagcgagtt cgacgccatc gtcctggcca
      841 tcggcgccct gcagggacgc gacctcgacg tacctggccg cgagctcggc ggcatccacc
      901 tcgcgatgga ctacctggtg cagcagaaca ggagggtggc gggcctcccg accaacactg
      961 aggtcgagat ctccgccgcc ggcaagaacg tcgtaatcct gggcggcgga gacacgagcg
     1021 cggactgcct cggcaacgcc caccgcgagg gctgcgcctc ggtaaaggta ctgacccacg
     1081 gcccccgtcc gcccgacacc cccgaccacc tcacctggcc cgactggccc ttcatcctgc
     1141 acacctaccc cgcccacgag gagggcggcg agcgcggctt ctccgtcgcc gtcaaaggct
     1201 tctccgggtc caacagccac gtcgaaagga tgcacgccat cgagaccgag cgttcttctg
     1261 acggcaagac catccccaga gaaggcacgg acttcgagat agaggccgac ctcgtgctgc
     1321 tcgccatcgg cttcacgggc cccgtacgtg accgcttcct cagagacatg gacctcggct
     1381 acaccgagcg cggcgccata cactccgaga acggcttcgg cactaaagag cctggcatct
     1441 tcgtcgccgg tgacgcccgg ctgggggccg acctgatcgt caccgccatc gccgagggcc
     1501 gcaaggccgc gaagcaggtt gacgagttct tgatgggccg cagtttgttg cctggatagt
     1561 ccacaggttt caggcaaccg cgaatacctg atacctattc gatactcgtt acactcgggt
     1621 acctaccgta taactagaag gttctcgacg gtcgactgga ggtgggactt atagagttct
     1681 cggttgagaa ggcgcgggag ttgttgctcg cggcgcaggg actgggcact gtaccgaaac
     1741 gtgccacgaa ggacgatgtt cgggagatca tccggcggat gggggtgttg cagatcgact
     1801 ccatctccgt cgtcgcgcgc agcccttatc tggtgctgtg gagcagactg ggctcctatg
     1861 agccgcggtg gctggacgaa ctgttagcag agggagcgct gttcgagtac tggtcgcatg
     1921 cggcgtgctt tatcccgatc gaggactacg gcctgtaccg ccggctaatg atcgagggag
     1981 gggagaagac cagaacctgg atgcgggccc atcatgagga gatcgagcac gtgatggagc
     2041 acattagtgg gaaaggtcct gtgcgcgccg cggagttcgc gcgcacggac ggaaaggccg
     2101 gaggctggtg ggagtggaag cccgagaagc gggcactgga gcatctgttt gcttccggag
     2161 agctcatgat ctcccgccgt gagaacttcc accgcgtcta cgacctgcgg gagcgcgtcc
     2221 tggcgagcgc gctgcctgac tgggaagacg cccgcgcgcc gaacgagcag gaggtgcggc
     2281 gggcgctggc cctcaaggcg gtacgcgcgc tgggcgtcgc cccggctcgc tgggttcctg
     2341 actacttccg cacgcccaag aagggtgtcg ccgggctgct agaagagctg gccgatgaag
     2401 gagaactcgt ccgcgcccgg atcgagggcg agcccgccta cgtgcacccc gacaacgcaa
     2461 ggctggccga gaagattctg tccggcaatc tgcagcctgc cttcacgacc ttgctatccc
     2521 cgttcgatcc tgtggtctgg gatcgggcgc gggctctaga gctcttcgac ttcgagtaca
     2581 agatcgaggt ctacaccccg gctgcccgcc gccggtacgg atactactct ctcccgatac
     2641 ttcacaacgg cgccctcgtt ggcaggctcg acgccaaggc ccaccgcaaa cagggaacat
     2701 tcgaagtcaa ggcgatacac ctcgagccaa atacgccggt gaacgaagac ctcatctccg
     2761 gtctctctgg cgctctgcgt gattgcgccg gctggcatgg aacgccggag gtggtggtcc
     2821 gccggtcaga accttcggat ctcacagaga cgctccggct cgcgctcgat gaggaaaccg
     2881 taccgcctac ccctggctcc acaaagacaa cctagccacg acgaagtcga caagatcgtc
     2941 ttcaggacag ccggaatctg agtctgggaa ccagccgatc cgcgcgtaca ctcgaattgc
     3001 atctgatagc gtaagcctgg aaaggggacg aatggacacc ggagtaaatc ccatcccgga
     3061 gggtttccac accgtgacgc cgtacatgac gatcaaggat gcagcgagcg ccatcgagtt
     3121 ttacaaagag gcgttcggag cgagggagat atttcgctgg gcagattcgg acgggcgcgt
     3181 caggcacgcg gagatcgtga tcggcgactc gccgatcatg ctcaccgatg aagcccccga
     3241 tttcggtatg aacagccccc aatcgctcgg cggctcgcct gtgcacgtgt tcctctacgt
     3301 cgaagatgtg gacgagctat tcggccgggc catcgcggcg ggggcgacgg agctgatgcc
     3361 ggtagagaat agcgaagacg gcgaccgccg cggcgggcta acggacccct tcgggcacgt
     3421 ctggtacatc ggcacgcata tcgaaggcat ctcccgcgag gaactccaga aacggtacga
     3481 cgcctttgca gaccggtaac cgtccgtggg tccggagaac gaagaaaagt cactaacgcg
     3541 aaagctacgc ccgccggctg gtcacgagtg ataatcgccg gcccggcgac cgacgtctga
     3601 taccaagtcc caagaagtga ggcatgtcag gcagccacga gatcgcttcg tatccgataa
     3661 ggactccaaa ggacgtggat tcccgctttc gcgggaacga cgcttaggga tgcctatccg
     3721 attgtgatgg gacggacctg gtatgactgc cccgaacgtc aacgcgaccg gacgagcgcc
     3781 tcgacgccgt ccagtatgcg ctgcagtccg aactcgaacg cgtactcggg gtcgtaggca
     3841 gacccgtaag catccccggc ggccggccct accctcgacg ccgtcgggta cagatccgga
     3901 tcgaagacct tctcgaggag cggctcgttt gccagccacc actcatcgtc ggtcttgccg
     3961 gtgcgcttct ccgcctcggc cacctccacc gaacgccgcg ccgcgccctg aacgtgctcc
     4021 aggacgaggg cgagaaccga atccatctcg acctcggtca gccctatccc gtccaccacc
     4081 cgaagctctc ggtcgtactt ggcgatcacg ttcggcccca gaggcggccg gctcgtcgcg
     4141 gctacctgca gcatccaagg gtgccgatga taaagcgccc agttatcgcg cgcgatcttc
     4201 tctaacccgg ccctccatcc caactctaca cttccagatt ccgccacctc gccaagcacc
     4261 gtatccagca tgacgtcgat aagctccgcc ttgccaggca cgtaggtgta gagggacatc
     4321 gtgccgatcc ccagcctgtc ggcgacgcgc cgcatggata acgccccaag cccctcggaa
     4381 tcggcgacct cgacggccgc gcccacgatc cggcccacac tcagcccggg cttcggtccc
     4441 ctggtcggct tcttcccgat cccccacagc agcgccatgg tacgctgcgg gtccccgcca
     4501 ccactgtact ccagcaccat ctcacacaac ctttccttgt aaatccgtac agcgtacagt
     4561 atagtgcata caactatgta ctacgtacgg agaaaggtga cgtcgtgaag ctgaaccatc
     4621 taaacctcac cgttacggac gttccggcgg cctgtgattt tctggagagg tacttcgatt
     4681 tgcgtaggat gggagggaac gccggcatgg ccttcctatc ggacgacgat gggtttgtgc
     4741 ttaccttgat gaaagctcgt agggccactg gaaacgccta tcccggcaac ttccacattg
     4801 gtttcttcgt ggagggcggc gagatcgtgg acgagatcaa ccgccgcttg aaggaagacg
     4861 ggttcgatgt ggcgccgcct gaacaacatc atgcctatac attctacgtc gaggcgccgg
     4921 gcgggttcac ggtcgagcta ggctcttaga ctgattcgag tcgagttatg gaggtggttg
     4981 tggaatcggc gaggttcgcg gtctttgcgg aggggctcga gaagcgttat gggaaggtga
     5041 aagctctgga cggcctggat ctccggatac caggagggaa ggtgtgtggc cttctcgggc
     5101 cgaacggggc agggaagacc acggccgtgc gtatcctttc gacgttgctc aggcctgaag
     5161 gcggccgggc tgaggtcgcg ggtttcgacg tggtgcgcca ggccgggcag gtccgatgca
     5221 gtataggtct ggcggggcag caggcagcgg tggatgagat cctcaccggt cgccagaacc
     5281 tggagacctt cggacgtctc taccaccttg gtgtgcggga ggcccgcgag agggcagacg
     5341 acctcctgga gcgtttcggg ctcgcggatg cggccggcaa gcaggcgaag aattattcag
     5401 ggggtatgcg ccggcggttg gacctcgccg ccagcttcat cctctccccg ccggtgctgt
     5461 tcctcgatga gccgactacc ggcctcgacc cgcgaggccg caacgaggtg tgggaggccg
     5521 tccgttcgct ggtcgccggg ggcaccacgg tgctcctgac cacccagtac ctggacgagg
     5581 ccgaccagct cgccgaccgg atctgcgtca ttgacgccgg gcgggcgata gcggagggca
     5641 cgccggaaga tctcaaatcc agggtcggcg gtgaccgcat cgacgtcgta gtgcgcagcg
     5701 ccggcgagtt gcccgtggcg gcgagggtgg tcggccgggt gtgcggggct gaaccggaga
     5761 tagaccagga atcccgccgg gtcagtgctc cggtgatgga tcgcttgtcc gccctgaccg
     5821 ggacggtgcg ggcgctggac gacgcggacg ttgatgccga ggatatcggg ctgcgccggc
     5881 ccacattgga cgacgtgttc ctaaggctca ccgggcaccg tgccggtgag aaagaagaag
     5941 aggtggcagc atgacacatc cgaccccggc gataccacgt agcgtgcccg atcgcctgag
     6001 gtgggccata gcggacgcct ggaccgtggc ccgtcgagac ctgatccact ggttccgcca
     6061 gcccttcggt atcatcgtca ccctgctgtt ccccgtgatg ctggtcctga tgttcggcta
     6121 cctcttcggc ggcgcgatct cactgccggg cgggaacgcc gcgggcggcg gtaaataccg
     6181 cgagttcctg atgcccggca tgttcgccat gaccatggcc ttcggcctgg aagcgaccat
     6241 gacgtcggtc gcgaccgacg cggccagggg cgtcaccgac aggttccgtt ccatgcccat
     6301 ggccccctcg gccgtggttc tcggccgcag catcgccgac atgatgagct ccgcgctcgg
     6361 tctctccgtc atgatcgcgt gcggcctgct gatcggctgg cggtggcacg agagcatcgg
     6421 ccaggcgctg ctcgccgtgg gactcctgct cctgctgcga ttctcattgc tgtgggtcgg
     6481 gatctttctg ggtctcatgg tcaagagccc ggaggccgta gcggcggtgc agatcctgat
     6541 ctggcccgtc ctcttcctgt ccaacacgtt cgtggcgcca gagacgatgc ccggctggct
     6601 cggcgctatc tccgagtgga accccctgtc ggccacagtc gccgccaccc gcgaactctt
     6661 cggcaaccct gggtggggcg gagagacctg gatctcgcag aattcactgc tcttggcagt
     6721 cttgtggccg acagtgatct tcgcagtctt cttcccccct ctccgtcaga cgctaccggc
     6781 gtctgagccg ctgacgagcg atcagggaac cggcgctctc gttacgctac ctttgtgttc
     6841 cagtcgatgt ggtagtcgtg catccacgcg agggatccac cgctggcaac gcgcttgagg
     6901 atcaggggca tcatcaggtc gcgcagcacc cgggcgacgg gaccggcggc cttgctattg
     6961 ctcgtcctcg ccccgtgggc gacgatgcgt tccaccctgg cccttcgtag tcgctcatag
     7021 acggcgaaag cctgcccggt gtccggcagg tcgcgcaggc acctggcgag cacgacagca
     7081 tcctcgatag ccatggaagc tccttgcccg gaggagggcg cagtggcgtg cgccgcgtcc
     7141 ccgatgatga tcatggaccc cctgtaccag gtcggtacgg agggaagatc gtaggtggcc
     7201 caaccggcga gttttccggg ggtggaacgg atgatctcga cggccggcga gtcgtccccg
     7261 gcgaagaggt cgcttaatat ctc
//
LOCUS       DATMVF010000292         4971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_82971, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000292 DATMVF010000000
VERSION     DATMVF010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82971"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VN178_15360"
     CDS             complement(<1..734)
                     /locus_tag="VN178_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWS82396.1"
                     /translation="MSRPEKALIVGGGVAGPVTAMAIRQAGIDSVVYEAYAGGADDAG
                     SFLTFASNGLDALRTIDAHHLVLSEGFPTPRMTIQSGSGKRLGDVPNGGTLPDGTVSQ
                     TLKRADLYRALRDEAVRRGARIEYGKRLVGAETTPGGVVARFEDGTAAEGDLLIGTDG
                     IHSRTRRIIDPSAPGARYVPVLNIGGFARGVTVQGEPGTFRMVFGKRAFFGYAVHPSG
                     EIWWFANPPRAAEPTRAELAAIGTEQ"
     gene            complement(731..1231)
                     /locus_tag="VN178_15365"
     CDS             complement(731..1231)
                     /locus_tag="VN178_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWS82397.1"
                     /translation="MKHETTTHYQIDEAKIRALFEDLLEDWGRGDGEAYGSRFTEDAD
                     YVAFDGTRTAGRQEISASHQRLFDKFLKGTRLTGRILSIKFPSPDVALIHATGGTVMR
                     GKTKPSPERDSIQTLVAVREGSEWRFAAFHNSRVRPIGGSAATFLLWAVTDKLWRIFA
                     PAKEGA"
     gene            complement(1429..2073)
                     /locus_tag="VN178_15370"
     CDS             complement(1429..2073)
                     /locus_tag="VN178_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS82398.1"
                     /translation="MASKRKVSNPLALAVMALLYERPMHAYEMVALMRERGKHESVRL
                     RYSSLYSVVGALEREGLIVPRETVREGRRPERTVYEITGTGREEFLTWLRELLREPVK
                     EYTQFAAGLSFLAALPPEEAVVLLRERVRRLERENEEMRLRLDAAMEQWNLPRLFVVE
                     SEHELILREAELGWVRGIVEEIEAEELGGLSEWRSFHSGQGSSGAAEGGEEAEP"
     gene            complement(2439..3179)
                     /locus_tag="VN178_15375"
     CDS             complement(2439..3179)
                     /locus_tag="VN178_15375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS82399.1"
                     /translation="MPEYDHLSDLYDLEYPHDYDVPFWLSIAQREARPVVEWGAGTGR
                     ISIPLAAAGHDVTAVEVSAKMVERGEEKSESVEWIVGDMRSVGPGRRYGMAICAFNSF
                     LCLRRVDDALAFLRNAHDHLVPGGLLGIEVSAFSPEELVDPPGRPAPRHDFTRMLPDG
                     WLDRFSISHYDAATQLMHMRLFYERYGPSGALESRRAHDLAIRVVGRSELELMLQLTS
                     FEVEAVYGGFEGEPFTSTSDHLIVLARK"
     gene            3318..3878
                     /locus_tag="VN178_15380"
     CDS             3318..3878
                     /locus_tag="VN178_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWS82400.1"
                     /translation="MGTVLLIVIVVVVVILVIYAIATYNGLVRRRNEVEESYRQIDVQ
                     LKRRYDLIPNLLEGVKKYFRQEQEVLTQITQARSQAMQPQSPGQQAQSEARLSGAIGN
                     LFAVAENYPELRSNTVLIDFQEELSSTENKISFARQHYNDSVQSYNTKIQSFPAVIFS
                     RAMGFTEQEYFEAQGPERESVTVSYD"
     gene            3883..4866
                     /locus_tag="VN178_15385"
     CDS             3883..4866
                     /locus_tag="VN178_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWS82401.1"
                     /translation="MNQRDQGTFRERIARNRRNSLLLIAAFLAFVTIFGYIIGFAWLG
                     DPTRALFGLALALVVGVVSGLVSYFAGDKMVLAASRAREITHDDASVLFNVVEEMSIA
                     SGLPRPKVYIVDDSAPNAFATGRDPEHATVAVTSGLLEKLDRDELQGVMAHEMSHVAN
                     FDIRYSMLVGILVGTTVLISDFFLRGLWLGGGRGGRRGGDGGGQLQIIMMLVAIVLAI
                     LAPLFARLLQLSISRQREFLADATAVRLTRNPKGLADALQKISGDREVLEVANRATAH
                     LYIVNPIKRFEKRSRGLFSTHPPIEERIMILRAMESGGVAETTGQTSTETP"
     gene            4892..4962
                     /locus_tag="VN178_15390"
     tRNA            4892..4962
                     /locus_tag="VN178_15390"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4924..4926,aa:Gln,seq:ctg)
BASE COUNT          905 a         1614 c         1505 g          947 t
ORIGIN      
        1 cactgctcgg tgccgattgc ggcgagctcc gccctcgtcg gctctgcggc gcggggaggg
       61 ttggcgaacc accagatctc gccggaggga tgaaccgcgt agccgaagaa cgcccgcttg
      121 ccgaagacca tccggaacgt accaggttcg ccctggacag tcacaccgcg ggcgaatcct
      181 ccgatgttga gcacaggaac gtagcgggca cctggcgccg acggatcgat gatgcggcgg
      241 gtgcgggagt gaatgccgtc ggtcccgatc aggaggtctc cttccgccgc ggtgccatcc
      301 tcgaaccgcg caacgacgcc gccaggcgtg gtctcggcac ccacgagccg cttgccgtac
      361 tcgatgcggg cgccgcgcct gaccgcctcg tcgcgtagcg cccggtatag gtcggcccgc
      421 ttgagggtct ggctgacggt gccgtcgggt agggtaccgc cgttgggaac gtcgccgagg
      481 cgcttgccgc tcccactctg gatcgtcatg cgcggcgtgg ggaagccctc tgacaacaca
      541 aggtggtggg cgtcgatcgt gcgcagggcg tccaggccgt tgctggcgaa ggtgaggaac
      601 gagccggcgt cgtccgctcc gcccgcatac gcctcgtaga cgacagagtc tattcctgct
      661 tgccttatcg ccatagcggt gaccggaccg gccacgccgc cgccgacgat cagggctttc
      721 tccgggcggc tcatgcgccc tcctttgctg gcgcgaagat gcgccagagc ttgtcggtga
      781 cggcccagag caggaacgtg gctgcgctcc cgcctatggg acgaacgcgg ctgttgtgga
      841 acgcggcaaa gcgccattcg ctgccctcgc gcacggcgac gagggtctgg atcgaatccc
      901 gttccggcga aggcttcgtc ttgccgcgca tcaccgtgcc tccggtcgcg tggatgaggg
      961 ccacgtcggg gctcgggaac ttgatgctca ggatgcgtcc tgtaaggcgc gttcccttga
     1021 ggaacttgtc gaagagtcgc tggtgtgagg cggagatctc ctggcgtcct gcggtgcgcg
     1081 tcccgtcgaa ggcgacgtag tcggcgtcct cggtgaaacg ggagccgtaa gcctcgccgt
     1141 ctcccctgcc ccagtcctcg agtaggtcct cgaagagcgc gcgtatcttc gcttcgtcga
     1201 tctggtagtg cgtggtggtt tcgtgcttca tcgggtcgaa aggtcctttc ttggtggggc
     1261 cggcggcgaa tgcgcgccgg ccatacctac cgcggagctt cgacctgtta tccttgaggt
     1321 tgcatgtctc gggtaacggg tctcgccctg cgcgggccta tttactcggg gattccgacc
     1381 cccggcatca ggtgttgcag cacccaccgg gggtcttctc tggtcagatc acggctcggc
     1441 ctcctctcca ccctcggcag cgcccgacga accctgccct gaatgaaagg agcgccactc
     1501 cgataggccg cccagctctt cggcctcgat ctcctctacg atcccacgca cccatcccag
     1561 ttcggcctct cgcaatatca gctcgtgctc cgactctacg acgaacaggc ggggtaggtt
     1621 ccactgctcc atggcagcgt cgagacggag tctcatctcc tcattctctc tttccagtcg
     1681 ccgcacccgc tccctaagta gcacgacagc ctcctcggga ggaagcgccg ccaaaaacga
     1741 gagaccggcc gcaaactgcg tgtactcctt gaccggctcc ctcaagagct cgcgcaacca
     1801 ggtgaggaac tcctcgcgtc ccgtgcctgt tatctcatag accgtccgct cgggacgccg
     1861 cccctcgcgt accgtctccc tcggcacgat gagcccctcc cgctccaacg cccctaccac
     1921 cgagtaaaga gagctgtacc tgagccgcac cgactcgtgc ttgccccgct cgcgcatcaa
     1981 cgcgaccatc tcataagcgt gcatcggccg ctcgtagagg agcgccatca ccgccaacgc
     2041 cagcgggttg gataccttcc tcttcgacgc caccagtgct cctttagtcg atataaacta
     2101 atcgaagtcg actatatact cggaatagcc tgatgtcaag ttgggaggtt tcaggcaatt
     2161 cagcatttca gcacgctccg gctgacgcct ccgcttgtca gcaagtcagc acttcagcta
     2221 gtgatgcgga atatgcatga ggactattgc ggtgtatgac ctgagggagt cgcactccct
     2281 cacgctcatc acgaccgtca ttcccgcgaa gcttgtcccc gcgaaagcgg ggagcgggaa
     2341 tccagacaga ggaggccaag cccaatggcg gactctcgtc gggcggttag catttacccg
     2401 gacttgtcat gagttcctga aaacgtgaga cctgaagcct attttctggc cagcacgatg
     2461 aggtggtcgc tcgtcgaggt gaacggctcg ccctcgaagc cgccgtagac cgcctctacc
     2521 tcgaaactcg tgagctgcaa catcaactcc aactcactgc gtcctacgac gcggatggcg
     2581 aggtcgtggg ccctccggct ctccagcgcg ccagagggcc cgtatcgttc gtagaagagc
     2641 cgcatatgca tgagctgcgt ggcagcgtcg taatgcgaga tggagaagcg gtcgagccaa
     2701 ccgtctggca gcatgcgtgt gaagtcgtgg cgtggcgccg ggcggcccgg cgggtctacc
     2761 agttcttccg gtgagaacgc ggagacctct atgccgagca ggccgcctgg cacgaggtgg
     2821 tcgtgggcgt tgcgcaagaa agccagggcg tcgtctactc tcctcaggca aaggaacgag
     2881 ttgaaggcgc agatggccat accgtagcgt cgtcctggac ctacactgcg catgtcgccc
     2941 acgatccact cgacgctctc gctcttttct tcgccccgct cgaccatctt cgcggagacc
     3001 tcgacggcag tgacgtcgtg gcctgctgcg gcgagtggga tggagatgcg acctgtgccg
     3061 gcgccccact cgactacagg tctggcttcg cgctgggcga tagagagcca gaacgggacg
     3121 tcgtagtcgt gggggtattc gaggtcgtag aggtcggata agtgatcgta ttccggcaat
     3181 tgagttagga gtcgagaact gtgaatcggg aactgttcat aacgtagccc tttccaactt
     3241 cgagattcac acttcacaat tctaacgtgc tgtggcggga tataatgcat ctggcatcct
     3301 agtcccggag gtattggatg ggtactgtct tgttgatagt catcgtagtg gtcgtggtga
     3361 tactcgtgat ctacgccatc gccacctaca acgggctcgt gcggcgacgt aatgaggtcg
     3421 aggagtccta caggcagata gacgtgcagc tcaagcggcg gtacgacctg atcccgaacc
     3481 tcctcgaagg cgtgaagaag tacttccgcc aggagcagga ggtgctgaca cagatcacgc
     3541 aggcccgctc ccaggctatg caaccccagt ctcctggcca gcaggcccag tccgaggccc
     3601 gtctctcagg ggcgatcggg aacctgttcg ccgtcgccga gaactatccg gagctgcgtt
     3661 ccaacacggt tttgatcgac tttcaggagg agctctcgtc gacggagaac aagatctcct
     3721 tcgccaggca gcactacaac gactcggtcc agtcctacaa caccaagatc cagagcttcc
     3781 ccgcggtcat cttctcccgt gctatgggat tcaccgagca agaatacttc gaggcccagg
     3841 gccctgagcg ggagtccgtg accgtctcct acgactagga ccatgaacca gcgggaccag
     3901 ggcaccttca gggagcgcat cgcccgcaac aggcgcaaca gcctgctgct catagccgcc
     3961 ttcctggcct tcgtcaccat cttcggctac ataatcgggt tcgcgtggct cggggacccc
     4021 acgcgcgccc tcttcggcct cgccctggcc ctggtagtgg gcgtcgtctc cgggctcgtc
     4081 agctacttcg cgggcgacaa gatggtcctc gccgcgtcca gggccaggga gatcacacac
     4141 gatgatgcgt ccgtgctctt caacgtggtc gaggagatgt ccatagcctc aggcctcccg
     4201 aggccgaagg tctacatcgt cgacgactcc gccccgaacg ccttcgccac gggccgagac
     4261 cccgagcacg ccaccgtcgc cgtcaccagc ggcctcctcg aaaagctcga ccgggatgag
     4321 ctgcagggtg tcatggcaca cgagatgtcc cacgtcgcca acttcgacat ccgctactcc
     4381 atgctggtcg gtatcctggt gggcaccacg gtcctcatct ccgacttctt cctgcgcggg
     4441 ctctggctcg gtggcggcag gggagggcgc aggggcggag acggtggcgg gcagttgcag
     4501 atcatcatga tgctcgtcgc catcgtcctc gccatactcg cccccctgtt cgcccgtctc
     4561 ttgcagctct ccataagccg ccagcgcgag tttctcgccg acgccaccgc cgtcaggctc
     4621 acccgcaatc ccaagggact ggccgacgcc ctgcagaaga tcagtggcga cagggaggtc
     4681 ctcgaggtag ccaaccgtgc gactgcccac ctctacatag tcaaccccat aaagaggttc
     4741 gagaagcgtt ccaggggcct cttttctaca catccgccga tcgaggagag gatcatgatc
     4801 ctccgcgcca tggagtcggg cggagtagcc gagaccacag gacaaacctc gacggagacc
     4861 ccataaagag atacaatatc cgccgttgca ttgccggatg gtgtaacggc agcacgagtg
     4921 actctgaatc acttagtcct ggttcgaatc caggtccggc agcttctaaa t
//
LOCUS       DATMVF010000293         9662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_591032, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000293 DATMVF010000000
VERSION     DATMVF010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591032"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VN178_15395"
     CDS             <1..1514
                     /locus_tag="VN178_15395"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWS82402.1"
                     /translation="MLIGLALLTRMVGLPDRVAFTASGLLLLPLRLTPASVTTPAGMA
                     RGPEMFFVSGIALVVSGVWLVVFNAGVLLWLVVGAFGWIKGLPPVLKMAVKYPTQSLF
                     RTGMTLAMFMLVVFTLTAMNFIQAAMSAAFGDTQGLSGGFEIRADAGYADPVPDMEAA
                     LRDTKGVERDDIGAVGQVSNLPAKVKQDGTDREPGSIFVQGVDGGYTDSVGYGFGATA
                     KGYGSARGVWDALQTEDAVVISSDLAPDRNASTFGPAVEPPVKLTGFYADDEKLPDDL
                     YLRVEDPDSGRAKELRVIGVLESSASFAGQVVTSQKTLEELAGGPVPPQSYYFDLRGG
                     ADAAATADALEKGFADNGLQTEVTAEVIRDSDATRRIVFLLLRGFMGLGLVVGICALG
                     VIAARSVVERRQQIGMMRALGFQRTQVRLAFLIESSFIALLGLGAGVILGLAFSGTLI
                     DNIREGFPGMEYRVPWSALLLVVVVGYAASLLTTYLPARRASRVYPAEALRYE"
     gene            complement(1738..1980)
                     /locus_tag="VN178_15400"
     CDS             complement(1738..1980)
                     /locus_tag="VN178_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82403.1"
                     /translation="MLNTLGDPARAAFRFTESLTMAQEIGETWTIALTLVGLAGVANA
                     QGEPDRAAQIFGAAAPLFESAGMVMAPPTAPTMIVT"
     gene            complement(2830..3033)
                     /locus_tag="VN178_15405"
     CDS             complement(2830..3033)
                     /locus_tag="VN178_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HWS82404.1"
                     /translation="MPYKQITQLPDSVKNNLPKHAQEIYKEAFNSAEDQYGEEDRAHR
                     VAWSAVEQKYEKNEKGNWVQKGN"
     gene            3286..3771
                     /locus_tag="VN178_15410"
     CDS             3286..3771
                     /locus_tag="VN178_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82405.1"
                     /translation="MAHDTRPRERHNVYLDGFPARESVRVVAMIYEDSGVKAHSHKCD
                     VRAHQVDRVCAFWVVLEGGTWTSPLTVCSPEGRAAIALFSGEEEAKMFCHFGKKRAKG
                     SVRVTSAGGVLSLLYGPWSVARHVALDPFPEVLGSRRLLEPLTLDRERFARSFAGGAG
                     S"
     gene            complement(4079..5464)
                     /locus_tag="VN178_15415"
     CDS             complement(4079..5464)
                     /locus_tag="VN178_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HWS82406.1"
                     /translation="MPSNTAARSPGLWETRLKAAKRVVGGAFRILVYLGVAIAPLVLA
                     WSGMEPGRGFLIDFSVALGFVGLSLMGLQFLLAARFRWVEAPFGMDVLLQYHRKIGYV
                     SLLFILAHPTLLFVADDEYLELLEVTDAPLLAKMGITSVVALLIMVALSVWRKKLRIG
                     YELWQWTHGVLGFVVVSAALAHVLMVGYYVNEPLEIALWLWMSVVFVGLVLWVRVVRP
                     LELRGKAWRVEDVVPERGGIHTLVLEPSRLRAREFDRFRFEPGQFAWITVGRSPFAIT
                     RHPFSISSSAEKTDRVAMSIKAAGDFTRKIGGLEPGATAYLDGPYGAFTVDRYNGPGF
                     VFIGAGVGITPLMSMLRTMADRGDPRPCYLFFGNREWEGVAFREEIDELKGRLDLEVV
                     HVLSRPPEGWEGEEGRIGAAVLSRHLPERYQSFQYFICGPDPLMDAVEGALVELGVPD
                     RRVHSERFAMV"
     gene            complement(5704..5892)
                     /locus_tag="VN178_15420"
     CDS             complement(5704..5892)
                     /locus_tag="VN178_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82407.1"
                     /translation="MNAKRLIAGLVAAVTLTLFMAAAGAQQPGAPQPGAPQPAAEQDD
                     DPADAFFDRIEEEEEEGD"
     gene            complement(6031..6444)
                     /locus_tag="VN178_15425"
     CDS             complement(6031..6444)
                     /locus_tag="VN178_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82408.1"
                     /translation="MSVFAPVAAAQGDDGGVDDRGRHARGTDDRGFDDRGMHARGADD
                     RGFDDKGMNNSAEDDNAAASPASSASASASPDASPSASPTASPSASPTASEGTTASAT
                     PVLPDTSGFPPAWVLGPLAMILGGGLMALGIARRR"
     gene            6793..8376
                     /locus_tag="VN178_15430"
     CDS             6793..8376
                     /locus_tag="VN178_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWS82409.1"
                     /translation="MEPPRLSGSLSGNSSVLVAAVLAALVVVWAATNLVLGGEGFAVL
                     VPDKVVGGEEAASALARLFAALVLGLFLADAVGWRARWVAGGLVVLGLGHFVFGYLEP
                     LIQEDPPELNESLYEAFVTQTLACALFMVGLFPGRPSRLVVWAATAIPVALVAGYVII
                     FEFLHGEEWMPLLSRVANPERTATLGSPLGWLTLWHWVLSMLPLGLAAAAVVGAFWQT
                     RRGLLRGWLLIAVVLLAGSVLHDYLWPSAYGGGLLTTADALSLAFAVVVAIGGISELR
                     RVASERARLLATERERAQRLNELHALRSDFSAMVAHELDTPIASVRKLNELLTVQGED
                     PEIRAYATNATERELDALANLVSDVRAVAAVEREGFGIEARRLPLEDLLADAEVYAST
                     LPGHHPIEVMVRGDLRAGVCVLADPERTGQVLRNLLSNAAKYSPEGTPIELRVIGKEG
                     QVRIEVADHGQGIHPEDVPRIFEKFGRGRDRERHKTPGVGLGLYLSRRIVRSHGSELT
                     VRTRVGVGSVFGFELAVVT"
     gene            8373..9041
                     /locus_tag="VN178_15435"
     CDS             8373..9041
                     /locus_tag="VN178_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS82410.1"
                     /translation="MSEEIRIMVVDDHDTFRDPLAFMLEREPNLTVVARPGSLSEAKE
                     ILESAELAVDVAIVDLNLPDGSGTDLIKDLRRTHPQAKALVLSAMSEQRYLAEAIEAG
                     AAGVMHKSAPMSDLVEAVRRLAAGDQLLSQQEVIGALRFLVRVRESNREAQLAIDKLT
                     PREREVLQALAEGWSDREIAQQLHVGAGTVHSHVTNILSKLEVSSRLQALVFAVRHGV
                     VTIE"
     gene            9214..9561
                     /locus_tag="VN178_15440"
     CDS             9214..9561
                     /locus_tag="VN178_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82411.1"
                     /translation="MTRRMAILVAVVGLMVMEFAGTALAVAKVGNAGPNRLVGTAEND
                     TLKGRGGADTIVGNGDSDRLYGGSGADHIKARERGRAEDDLVDCGRGRDTVLTDNTTE
                     DTISSNCEVVRRG"
BASE COUNT         1733 a         3024 c         3068 g         1837 t
ORIGIN      
        1 tgatgctgat cgggctcgcc cttctgacgc gcatggtggg gctgccggac cgcgtcgcct
       61 tcaccgcgtc cggacttctc ctgctccccc tgcggcttac cccggcctcc gtcaccacgc
      121 cggctgggat ggcgcgcggg ccggagatgt tcttcgtctc cggtatcgcc ctcgtcgtct
      181 cgggcgtctg gctcgtcgtc ttcaacgccg gcgtgctcct gtggctggtc gtgggggcgt
      241 tcggatggat caagggacta ccccctgtcc tcaagatggc ggtgaagtac ccgacccaga
      301 gcctcttcag gaccgggatg acgctggcga tgttcatgct ggtcgtcttc acgctcacgg
      361 cgatgaactt catccaggcc gcgatgtcgg ccgcgttcgg ggacacgcag gggctctcgg
      421 gcgggttcga gatccgggcc gacgcaggct acgccgaccc ggttcccgac atggaagccg
      481 ccctccggga caccaaaggc gtggagagag atgatatcgg tgccgtgggc caggtttcca
      541 acctcccggc gaaagtcaag caggacggta ccgaccgaga gccgggatca atcttcgtgc
      601 aaggggtgga cgggggctac acagatagcg tcgggtacgg tttcggggcg accgccaaag
      661 gctacggctc ggcgcgcggg gtgtgggatg ccctccagac cgaagacgcg gtcgtgatct
      721 cctcggacct cgcgcccgac cgtaacgcct cgaccttcgg tcccgcggtg gagccgccgg
      781 tcaagctgac cggcttctac gccgacgacg agaaactgcc ggacgatctc tacctgcgag
      841 tggaggatcc cgattccggg agagcgaagg agctgcgcgt gatcggggtg ctcgaaagct
      901 cggcctcctt cgccgggcag gtcgtgacct cgcagaagac gctggaagaa ctcgccggag
      961 gccccgtccc gccacagtcc tactacttcg acctgcgcgg gggcgcggac gcggcggcca
     1021 ccgccgacgc cctggagaaa ggcttcgccg ataacggctt gcagacggag gtcacggccg
     1081 aagtcatccg ggacagcgac gcgacgaggc ggatcgtctt cctgctcttg aggggcttca
     1141 tgggcctcgg gctagttgtt ggcatctgcg ctttgggcgt gatcgcggcg cgctcggtgg
     1201 tcgagaggcg ccagcagatc gggatgatgc gggccctggg cttccaaagg acccaggtgc
     1261 ggctcgcgtt cctgatcgaa tcctcattca tagccctgct cgggctgggc gccggagtga
     1321 tcctggggct cgccttctcg ggcacgctga tcgacaacat ccgggagggg ttccccggca
     1381 tggagtaccg ggtgccgtgg agcgccctgc tgctggtcgt cgtggtcggc tacgccgcct
     1441 cgctcctgac cacctacctg ccagcccgac gggcctcacg agtctacccg gcggaggcgt
     1501 tgcgctacga gtaggggagg cgtgatcgag gggccgaatc cgcaagcgcg accattggtt
     1561 gctgcccgtt tggtctcctt tacctgcttc gcgagggctg cgcctgcagt acatcaaggg
     1621 ctttggcacc ctctggccgt ctccagggcg taggtgatcg cttgttcggt cgtcagtgcc
     1681 cgcccgccgc gctcatcgcc gcgaaggtcg ttgcgtcgag tttggtccgt gtggcggtca
     1741 ggttacgatc atagtcggcg cagtcggcgg agccatgacc atgccggcgg actcgaacaa
     1801 cggcgcagcc gccccgaaga tttgcgccgc tcgatccggc tcgccttgcg cgttcgctac
     1861 cccggcaagc cccacgaggg tcagggcgat ggtccacgtt tcgccgatct cttgcgccat
     1921 cgtcaggctc tccgtaaagc ggaaggccgc tcgcgccggg tcgcccaggg tgttgagcac
     1981 gagggcgacg ctgcgcttgt ggcctaggtt ccgcgagagt tagtccctcc tcgagcaacg
     2041 cccgcgaccg gtccttctcc ccctggccga gctccacgcg ggccaggcag aaagtgtaga
     2101 gacgagccgc ttacttgtag aatcgatgag tgcagggttt ccgaacatgg gagcttcgaa
     2161 gtgcctgatt ctgcaacacc cgccgggcgt ccaccgtatt ggtccgactg cgagaacatg
     2221 aaacttcctg agcttgggcg gttcatctac gacgccctcc tccctcaggg tggagtggaa
     2281 agaagcccca ctacaacatt agctcggtgc ggttctaggt cactctggtc gcgacgtact
     2341 cggagtgggt cgacaagtac aggagttatc cgcggggagt gggggccggg ctattcggga
     2401 agggggagtg ctcctgtgtc gaaaaagatc gagtacccac agttggtaca cgtagcgtag
     2461 ttgaattggg actctgggga agtaggggga cctgaggacg taagtgggtc tgaactggat
     2521 cctgccaggg tgcctctggc ctcgaagctc tccgtgacct tacatatagg gcaaaccaga
     2581 ggtcccgctt tcttgctgtt caagtgcgcg gcaaatctgg tgtacccgta gctgtcgtcc
     2641 ataatgtctc ctttactcaa atatctcaga ggcatactaa ccgatttccc tttcgtaatg
     2701 aatatcggag tatcctactt ctcgtcctga tgctccggtc catttcttga ggagttgcgg
     2761 gctgctaggg actagcgcta ccccctcaca gccaacgtcg cacgtacgcg catgtcataa
     2821 aaacagaggt tagttaccct tctggaccca gttgcccttc tcgttcttct cgtatttttg
     2881 ctctacggcg ctccaggcga ccctatgggc acgatcctct tcaccgtact gatcttccgc
     2941 ggagttgaag gcctccttgt agatctcctg ggcgtgcttg ggtaagttgt tcttcacgct
     3001 atccggtaac tgcgtgatct gtttgtaggg catctcgatc ccctttcgtg gcttgcccga
     3061 agatacttac ccacttcgta cgtccggcca accgaaccgt caaggttctg tcgagagccc
     3121 tgtgggccgg tgccaggaac ctcgcctgtc taggtctcta ccgtccctca acgcttggcg
     3181 gggtcctcaa gctggaaggt gatctccacg ttgaccttgt agccggtgat gttgccgttc
     3241 tcgatcatga ggtttgagta tcgagatgca cccaagttgc acactttggc ccatgacacg
     3301 cgcccccgcg agcgccacaa tgtctacctg gacggatttc cggctcgaga aagtgtgcgg
     3361 gtggtggcga tgatctacga ggactccggc gtgaaagcac actcgcacaa gtgtgacgtg
     3421 cgtgcccacc aagtcgatag agtttgtgct ttctgggtcg tgctcgaagg tggcacctgg
     3481 acgtcccctc ttacggtatg ctcacccgag ggcagggcgg cgatcgcgct cttcagcgga
     3541 gaggaagagg cgaagatgtt ttgccacttc ggaaagaagc gggcgaaagg aagcgttcga
     3601 gtgacctccg cgggtggggt gctctcactc ctgtacggcc cctggtccgt ggcaaggcac
     3661 gtggccctcg acccattccc cgaggtcctt ggtagcagga ggctcttgga accgttgacg
     3721 ctcgacaggg agcgcttcgc ccggagcttc gcaggaggag cgggttccta gctgttcgcc
     3781 cggacgccgc gcacggcgac cctaccagag agggccatcg gcgacccgga cgcgctcctc
     3841 ttggtgacga acaaactcgg gtgaatagtg ccggaacggg tccgatgcac atttcggccc
     3901 acgacgcccc acctggcaaa catgacaatg gtagaacact cggcccgaag ccacgttatc
     3961 ccccctcatc cgccctgccc caacgcgaag agaacgcaaa cgaggatcag cagcgccgtg
     4021 gtaagcaccg tgaagactgt tgagtaagtc tgtcgcatct cctaacccta ctcggtactc
     4081 acaccatggc gaaccgctcg gaatggaccc tcctatcggg taccccgagc tcgaccaggg
     4141 caccctccac ggcgtccatc agagggtcgg ggccgcagat gaaatattgg aagctctggt
     4201 atcgctcggg caggtgcctc gaaagcaccg ctgctccgat acggccttct tctccctccc
     4261 atccctcggg cgggcggcta agtacgtgca ccacctccag gtcgagccta cccttgagct
     4321 catcgatctc ctcgcgaaaa gcaacgccct cccactcgcg gttaccgaag aacaggtaac
     4381 aaggcctggg atccccccgg tcggccatcg ttcggagcat gctcatcagc ggggtgatac
     4441 cgacccccgc gccgatgaag acgaagccgg gaccgttata gcggtccacg gtgaacgctc
     4501 cgtaagggcc gtccaggtag gccgtggcgc cgggctcgag gccgcctatc tttcgggtga
     4561 agtcgcctgc ggccttgatg ctcatggcca cccgatcggt cttctcggcg ctcgaggaga
     4621 tcgagaacgg gtgacgggtg atcgcgaagg gagatctgcc gaccgttatc caggcgaact
     4681 gccccggctc gaagcggaag cggtcgaact cgcgggcgcg aagcctcgaa ggctcgagca
     4741 cgagggtgtg gataccaccc cgctccggga ctacgtcctc gacacgccac gctttgccgc
     4801 ggagctccag cggcctgacc acgcgcaccc acagcaccag gccgacgaac accaccgaca
     4861 tccagagcca cagcgctatc tccaggggct cgttcacgta gtaaccgacc ataagtacgt
     4921 gcgcgagggc ggcggacacg accacgaacc cgagcacgcc gtgcgtccat tgccagagct
     4981 cgtagccgat gcgtagcttt ttcctccaca cggagagggc taccatgatg agcagcgcca
     5041 ccaccgaggt gatccccatc ttcgccagca gtggtgcatc ggtcacctcc aggagctcga
     5101 ggtactcgtc atcggccacg aacaggaggg tagggtgggc caggatgaac aggaggctta
     5161 cgtagccgat tttgcggtgg tactgcagca agacgtccat accgaacggc gcctcgaccc
     5221 accggaaacg ggcggcgagg aggaactgca ggcccatcaa ggacaggccc acgaagccca
     5281 acgcgacgga gaagtcgatc aggaagcccc tccctggctc catccccgac caggccagga
     5341 cgagcggggc gatggccacc cccaggtaca cgaggatccg aaacgcccct ccaaccaccc
     5401 gcttcgcagc cttgagtcgg gtctcccaca agccgggcga gcgcgctgcg gtattgctcg
     5461 gcactctacc gctgcgctct cgattctttc cagcttcgac tcgcggttcg gcacttttca
     5521 cactatcgtt tcccaaacta gctccctaat ctctcgcccg ccgacgcact cgggtaccgg
     5581 tagaagggca accgtgccga ccgagatgtg cgaccaagag cgggtgctcc accggccgac
     5641 gtcccgaacg ccaaaaccgg gcaacaacac ttgaacttac gcctgcccgt gttacgcacg
     5701 cgttcagtcg ccttcctctt cctcctcttc tatgcgatcg aaaaaggcgt ctgccgggtc
     5761 gtcatcctgc tcggcagctg gctgcggagc ccctggctgc ggagcccctg gctgttgagc
     5821 acccgcggct gccatgaaca gcgtcaaggt taccgcggcc accaaaccgg ctatcaacct
     5881 cttcgcgttc atccttctcc tttctgagcc ctttctgaca tggcggtgct gattgtgggc
     5941 gtccggggcg ggctgtacat ctgcaaaatt ggggtccttc tccaaagagg gggaagcgtt
     6001 agggcttccc ccctcatatg ccggagtgtt ctagcgacgg cgcgcgatac cgagggccat
     6061 cagccctccg ccaaggatca tcgccaacgg cccgaggacc catgccggag ggaagccgct
     6121 ggtgtccggt agtacgggtg tagcagatgc cgtagtgcct tcagaggccg taggactcgc
     6181 gctgggtgaa gccgtcgggc tcgcactagg cgaagcgtca ggactcgcgg aagccgaggc
     6241 actggaggcg gggctggccg ccgcgttgtc gtcttcggcg ctgttgttca ttcccttgtc
     6301 gtcgaagccc ctgtcgtcgg caccccgagc atgcatcccc ctatcgtcga agccacgatc
     6361 atcggtccct cgggcatgcc tgcccctgtc atctacgccc ccgtcatctc cctgggcggc
     6421 cgcgacgggt gcgaagaccg acacggcaaa caccaccgac aagacaagta atagccatct
     6481 acgcatcgtt tctccacttt ttcgcttgta catccacgaa aaggatgcac cacccaggtt
     6541 cgtatgacct atacaaattt atggatcttt tccaaccgct gtgacgacgc gtccgatggc
     6601 ggttactcct cgagtcgttc cctcgcgtgg atgcccgggc gaccctcgac cggttcgcga
     6661 aaccatctct agcgtgccat cctgcgtatg tacggaaggg agactacact ctggtctcct
     6721 ttcttgaagg ctgcagaaga tcgcagagga ggttcgttgg gagcgaaggg ccactctcga
     6781 gagagtacga cagtggagcc gccgaggctg tcgggttccc tgagcggcaa cagtagcgtg
     6841 cttgtcgccg cggtgctggc ggctttggtc gtggtgtggg cagcaacgaa cctggttttg
     6901 gggggtgagg ggttcgcggt gctcgtcccg gacaaggtgg tgggtgggga ggaggcggcc
     6961 tctgctctgg ctcgcctctt cgccgccctc gtcctcggtc tgttcctggc tgacgccgta
     7021 ggttggcgtg cccgctgggt ggcgggggga ttggtggtcc ttggcctggg acacttcgta
     7081 ttcgggtacc tggagccgct gattcaggag gatcccccgg aactcaacga atcgctctat
     7141 gaggccttcg tcacccagac gctggcgtgc gcgctcttta tggtaggtct gttccccgga
     7201 aggccgtctc ggctcgttgt atgggccgca acggcgatcc cggtcgccct ggtagccggc
     7261 tacgtgatca tcttcgagtt cctacatgga gaagagtgga tgcctctcct gtctcgcgtc
     7321 gcgaacccgg agagaacggc gacgctcgga tcgcccctgg ggtggctgac tttgtggcac
     7381 tgggtactct ccatgctgcc gttaggtctt gcggccgcgg ccgtggtcgg ggccttctgg
     7441 cagacccgcc gtgggctact gcggggctgg ttgctcattg cggtggtgct cctggccggg
     7501 tcggtcctgc acgattacct gtggccttcg gcctacgggg gcggcctgct caccaccgcc
     7561 gacgccctca gtttggcctt cgcggttgtg gtcgcgatcg gtggcatctc cgagcttcgg
     7621 cgcgtcgcct cggagcgcgc aaggcttctg gctaccgagc gggaaagagc gcagcgactc
     7681 aacgaactgc atgccctcag gtcggacttc agtgcgatgg ttgcccatga gttagacaca
     7741 cctatagcct ccgttcgcaa gctcaacgag ttgctcaccg tccaggggga ggatcccgag
     7801 atacgcgctt acgctacgaa cgctaccgag agagaactcg acgccctagc caacctcgta
     7861 agcgacgtac gggccgtggc tgcggtcgaa cgcgagggtt tcgggatcga ggcgcgccgt
     7921 ctgccgttgg aagatctcct ggccgacgcc gaggtgtacg cgagtaccct acccgggcac
     7981 catcccatag aggtgatggt ccgaggggat ctcagggcgg gcgtttgcgt gctcgccgac
     8041 cccgagcgta ccggtcaggt cctgcgcaac ctgctctcga acgccgccaa atactcgcca
     8101 gaaggcacgc ctatcgagct gcgggtgatc ggcaaggagg ggcaagttag gatagaagtc
     8161 gccgatcacg gccagggcat ccacccggaa gacgtgccac ggatcttcga gaaattcggc
     8221 cggggccgcg atcgggagag gcacaaaacg ccgggagtag gcctcggcct ttacctcagc
     8281 cggcgcatcg tccgaagcca cggttccgag ctaacggtgc gaacgagggt cggcgtgggc
     8341 tcggtgttcg ggttcgaact ggcggtggtg acgtgagcga agagatcagg atcatggtcg
     8401 tcgatgacca cgacactttc cgcgacccac tcgcgttcat gttggaacgc gagccaaatc
     8461 tcaccgttgt ggctcggcca ggatctctat ccgaggccaa agagattctc gagagcgcgg
     8521 agcttgcggt ggacgtggcg atagtcgacc tgaaccttcc cgacggttcg ggtacggatc
     8581 tcataaagga cctacgccgt acccatccgc aggccaaggc gctggtcttg agcgccatgt
     8641 ccgagcaaag atacctggcc gaagcgatag aggcgggcgc ggcaggggtc atgcacaagt
     8701 cggcacccat gagcgatctc gtggaagcgg tgcgccgcct ggccgccggg gaccagctgc
     8761 tctcccagca ggaggtcatc ggggccctgc ggttcctcgt gcgcgtgcgc gagagcaatc
     8821 gcgaggcgca gctcgccatc gacaagctga cccccaggga acgcgaagtg ctccaggccc
     8881 tcgccgaggg ctggagcgac agggagatcg cacagcagct gcacgtcggc gcgggaacgg
     8941 tgcactctca tgtgacgaac atcctctcca agctcgaagt ttcctcgagg ctgcaagcgc
     9001 tcgtcttcgc cgtgcgccac ggcgtggtaa cgatcgagta aacggtcttg gtcggaccct
     9061 gaactcgtgc tcgcagagga ttctagaagc ccctccttct atgcaaattt gcatagacga
     9121 atatcgacaa ttctgaggat ggcgctagtc ttccgaggag ccatcatatg ctttgtcggt
     9181 ataagacaac gcagcatcga aggaggtaag cgagtgacga gaaggatggc catattggtc
     9241 gcggttgtag ggcttatggt gatggagttc gccgggacgg ccctggcggt cgcgaaagtc
     9301 ggcaacgccg gtcctaacag actggtaggc accgccgaga acgacaccct caagggccga
     9361 ggcggggctg acaccatcgt cggcaatgga gactcggacc gtctctacgg tgggagcggt
     9421 gcagatcaca tcaaagcccg cgagcgtggg cgcgccgaag acgatctggt cgactgcggt
     9481 cggggccgcg acacggtcct aaccgacaac acgaccgagg acaccatctc gtcgaactgc
     9541 gaggtagtta ggcgaggtta gaaccctgcc gcagagacac tcagggccgg ggccgctagc
     9601 ggctccggcc cttcttctgc ccaacctcga agatggacat ctctccgaaa ctcggggaga
     9661 tg
//
LOCUS       DATMVF010000294         5963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_229448, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000294 DATMVF010000000
VERSION     DATMVF010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229448"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..791)
                     /locus_tag="VN178_15445"
     CDS             complement(207..791)
                     /locus_tag="VN178_15445"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWS82412.1"
                     /translation="MEGRSTERVAVFVDGANLYHSIKNYYKGVLDYARLLSAAVGDRK
                     LLRATFYIVEKQEADEGQGTTSARSFVYNLNKFGYKVRSKPLMVHETLTPDGERTISH
                     KGDWDIGVVVDMMRLAEHADTYVLVSGDGDYVEAVEFLQSERGIRVEVISAAQCTSQA
                     LLDVCDAHTDLADIPELFRRPGPPKQAVSDQQVS"
     gene            947..1255
                     /locus_tag="VN178_15450"
                     /pseudo
     CDS             947..1255
                     /locus_tag="VN178_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
     gene            1302..1520
                     /locus_tag="VN178_15455"
     CDS             1302..1520
                     /locus_tag="VN178_15455"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HWS82413.1"
                     /translation="MKKTTVYLPEDLKSALGRLATQRGRSEAELIREAIRDLVEDSEP
                     PRPRLPLFSSGDPTLARRFEEELHGFGE"
     gene            1544..1942
                     /locus_tag="VN178_15460"
     CDS             1544..1942
                     /locus_tag="VN178_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HWS82414.1"
                     /translation="MLAAIDERQEDYIAAREVVESARGPLLISPFVLAELDYMILTRM
                     GRGYELALLGEVARGVYGLEYFSEDDVAEAVEIVERYADFRIGLADASNVVLANRHDT
                     LDVLTLDERHFRTLRGPGGRPFRVLPADAG"
     gene            1996..3552
                     /locus_tag="VN178_15465"
     CDS             1996..3552
                     /locus_tag="VN178_15465"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWS82415.1"
                     /translation="MMADIKKLEVPESLSRLGEAFREAGHELYFVGGVVRGALLLGSD
                     KDPATEAQIDRRDADAATDAHPARIKALLRPHAENLWTVGERFGTIAATIGEYEVQIT
                     TYRTDQYTRGSRHPEVRFGDSLREDLARRDFTINAVAADALTGEVVDPFEGRKDLERG
                     VIRAVGDPLERMRDDPLRMLRAVRFETTLTQPEKPFAMTQDLEEAIRNNAGWLESISA
                     ERKREEFEKILISENVASGLRALVRLGLMPYVVPEFMETLDVEQETEFHHKDVFEHTL
                     IVVSSIEAEPILRKAAFFHDIGKPRTLVYEHRCTYCGAKSTRKSAEEGECERCAGRTI
                     PRKIHFYGHENVGAAIARRAMQRLAYPKDDVDAVAHLVANHMRPYGYAASKDPWSDAA
                     VRRFIRDTYLARGDRELANVDMLLRLARADITGSAPRRRRIAEESWRSLKRRVEEVRA
                     EDAVEKLESPLSGNDLMEQFGLGPGRWIKALKDYLQNEVVEGRLGKDDKERARGLAEA
                     YAREHDILEE"
     gene            3556..4434
                     /locus_tag="VN178_15470"
     CDS             3556..4434
                     /locus_tag="VN178_15470"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWS82416.1"
                     /translation="MEKQDPLVTTDWLAAHLDDEKMRVVDIRGYVEKTDLGGGRQKAD
                     YLAAREEYDEAHIPGAVYVDWTSDITDPDDPVPVQIAPPERFADLMGSLGIGDDTSVV
                     VYDHAGGQFATRLWWALTYYGHDGVFVLDGGWRKWTAEDRPTTAEVPEPEPAVFTPEP
                     QSGWRTDAEGLLATSDGGDALVLDARDEGQYTGAVTREEARTGRVPGAAHLHSDSLFD
                     PESGTFHSDEELARRLEEAGVSGDRDEPVVAYCNGGVAATVPLFALHRLGYKRLANYD
                     GSWNEWGNREDLPAER"
     gene            4486..5142
                     /locus_tag="VN178_15475"
     CDS             4486..5142
                     /locus_tag="VN178_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWS82417.1"
                     /translation="MKTYNVHRSRVRNIVSWVLSLLMIAAGVALIASFFLAGRLDSTA
                     TNSDNPGGFNVPKLETTDEENTSSTGPSDKTLELTVPAMARIENDEVPYTAGNDEEAL
                     KTHAAIHLEGTGFPWDNEANVYIAGHRLGYPNTESWLTFWDLSNVVGGDKIYVTDANG
                     TEYTYDVFKTFVVSPSATQVTDTLPGKNILTLQSCTLPDYGKRLIVQAELTETSEKAA
                     "
     gene            5154..5858
                     /locus_tag="VN178_15480"
     CDS             5154..5858
                     /locus_tag="VN178_15480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS82418.1"
                     /translation="MDEGLLEGQLAYYRARAGEYDEWFLRTGRHDRGPEWNRTWFLEL
                     ERVRQELDRFGPTGRVLELACGTGLWTVELARHAAGITAVDASPEVLDINRARLQETL
                     PEATVSYVRADLFGWRPEESYDVVFFGFWLSHVPPGRFAAFWDLVRSALRPGGRVFFV
                     DSLRTETWAEKESAGRGPRDHTTLRRLNDGREFSIVKVFYDPVELEARLAGMGWRISV
                     RTTEKHLLYGFGEYKR"
BASE COUNT         1191 a         1826 c         1916 g         1030 t
ORIGIN      
        1 cgccagaggg tcgtcgagag actgggcatc gagctggagc cggaagtaag ggtattaggg
       61 gagccgtagc atttcagcac gcctgcttcg caggcttgtc agcacttcag catttcagtt
      121 tgctcacacg aaagctcttg ctgacatgct gacaaggcgc caacggcgcc gtgctgacaa
      181 gcggaggcga agccggagcg tgctgactag ctgacttgct gatcgctgac agcctgtttc
      241 gggggccccg gcctcctgaa caactccggt atgtccgcca ggtcggtgtg ggcgtcgcag
      301 acgtccagga gggcctggga agtgcactgg gccgcgctta ttacctcgac gcggatgcct
      361 ctttcgctct ggaggaactc gacggcctcg acgtagtctc cgtcgccgga gacgaggacg
      421 taggtgtcgg cgtgctctgc gaggcgcatc atgtccacca ccacgcctat gtcccagtcg
      481 cccttgtgcg agatggtcct ctccccgtcc ggggtcaggg tctcgtgcac catgagaggc
      541 ttggagcgta ccttgtagcc gaacttgttg aggttgtaga cgaaggagcg cgccgaggtc
      601 gtcccctgtc cctcatcggc ctcctgcttc tcgacgatgt agaaagtcgc cctaagtaac
      661 ttcctgtctc cgacggccgc cgagagaagc ctggcgtagt cgaggacgcc cttgtagtag
      721 ttcttgatcg agtggtagag gttggccccg tcgacgaaga cggccaccct ttctgtactt
      781 ctcccttcca tcacgcccga atcttagcag aaaacattgc atgcccgtac aaagcaggcg
      841 gtttaccggg cacacgaact acgctaatat ccattggccg ctctgcagcg actctagaga
      901 ggagtgagcg gacacggact tcgaggccag cttagccgaa catgccgagg gatactctgt
      961 cgtctcggtg cggggtgaga tggacctgca taccgccccg aaggtcgagt acgctataga
     1021 gagggccgtc gagaacaagg acgttggtgc cgtcgtggtt gacctgggcg aagtcacatt
     1081 tatggactcg acagccctct cggccctcat gcgctccaaa gacgccttag ggcaaaaggg
     1141 gatctccttc cgtctcgccg ccccctccaa agccgtcgaa cgtatcttct ccgtgacagg
     1201 cttccaggac ttcttcgaga tcttcccctc ccgcgaagcc gccatccccg tctgagttcc
     1261 aggcaaatga tccatccctg acgtatggta aaacgtatgg catgaagaag accacggttt
     1321 atctgcctga ggatctcaag tctgcgctgg ggcgccttgc tacgcagagg gggcgcagtg
     1381 aggccgagct catacgcgag gcgatcaggg atctggtcga ggattccgag ccgccgcgcc
     1441 cgcgattgcc cctattttcg agtggggatc caacactggc gaggaggttc gaggaggaac
     1501 ttcacggttt cggcgagtga cgctcatcct ggatagcagt ggtttgctcg ctgccataga
     1561 cgaaaggcag gaggactaca tcgctgccag ggaggtagta gaatccgccc gcgggccgct
     1621 cttgatctcc ccctttgtat tggcggaact cgactacatg atcctgaccc gtatgggacg
     1681 gggctacgaa ctggcgttgc tcggcgaggt cgcacgcggt gtgtacggtc tggagtattt
     1741 ctcggaagac gatgtggccg aggctgtcga gatcgtcgag cgttatgccg actttcgcat
     1801 cggcctcgcc gatgcttcca acgtggtgtt ggcgaaccgc cacgacacgc tcgacgtgct
     1861 cacgctcgat gaacgccact tcaggacctt gaggggacct ggcgggcgtc cgtttcgtgt
     1921 tctgccggcc gacgccggtt gatcttcgcg agcgtctgtg acctgcagtc tgttgcgtac
     1981 tagaatctgg tcattatgat ggccgacata aagaagctgg aggttccaga gtctctctcg
     2041 cgtctcggtg aggcgttcag ggaggccggg cacgagctct actttgttgg cggggtcgtg
     2101 cggggagcgt tgctcctggg ctctgataag gatccagcca cagaggcaca gatcgatcgg
     2161 cgggacgcgg acgcggcgac cgacgcgcat cctgcgagga tcaaggcatt actcaggcct
     2221 cacgccgaga acctgtggac ggtgggggag cgcttcggga ccatcgcggc cacaatagga
     2281 gagtacgagg ttcagatcac gacctacagg accgaccagt acaccagggg gagcaggcat
     2341 cccgaagtaa ggttcggaga tagtctgcga gaagacctgg cacgcaggga cttcacgatc
     2401 aacgccgtcg ccgccgatgc gctcacgggt gaggtcgtag atcctttcga gggccgcaaa
     2461 gatctggagc ggggtgtgat ccgggccgtc ggcgaccctc tcgaaagaat gcgcgacgac
     2521 cccttgagga tgctgcgggc cgtccgattc gagacgaccc ttactcagcc cgagaagcct
     2581 ttcgccatga cgcaggacct cgaggaggcg atccgcaaca acgccggctg gctggagagc
     2641 atctcagccg agcgcaagag ggaagagttc gagaagatcc tgatctcaga gaacgtggcg
     2701 tccggtctga gggcgctggt gcgtctcggg ctcatgccct acgtcgtgcc cgagttcatg
     2761 gagaccctcg acgtggagca ggagaccgag ttccaccaca aggacgtctt cgagcacacc
     2821 ctgatcgtgg tctccagcat cgaggccgag ccgatcttgc gcaaggcggc tttcttccac
     2881 gacatcggga agccgagaac gctagtctac gagcaccgct gcacctactg cggggcgaag
     2941 agcacgagga agagcgccga ggagggggag tgcgagcgtt gcgcagggcg cactatcccc
     3001 aggaagatcc acttctacgg ccacgagaac gtgggggcgg ccatagcgcg ccgggcgatg
     3061 caacgcctcg cctaccccaa ggacgacgta gacgccgtcg cccacctcgt cgccaaccac
     3121 atgcgtcctt acgggtacgc cgcgagcaag gacccctgga gcgacgccgc tgtaaggcgc
     3181 tttatcaggg acacctacct cgcgcgcggc gacagggagc ttgctaacgt ggacatgctc
     3241 ctcaggctcg ccagggccga catcacgggg agcgcgccgc gccgcaggcg catcgccgaa
     3301 gagtcctggc gcagcctcaa gaggagggtc gaggaggtaa gggccgagga cgccgtcgag
     3361 aagctggaga gccccttgag cggcaacgac ctcatggagc agttcggcct tggaccaggg
     3421 cgttggatca aagccctaaa ggactacctg cagaacgagg tggtcgaagg gcgcctcggc
     3481 aaagacgaca aagaaagagc ccgcgggctc gccgaagcct acgcccgcga acacgacatc
     3541 ctggaggagt agaaagtgga gaaacaggac cctcttgtca ctactgactg gctcgccgca
     3601 catctcgatg acgagaagat gcgcgtcgta gacatcaggg gttacgtaga gaagacggat
     3661 ctcggcggag ggcgccagaa ggccgattac ctcgccgccc gcgaggagta cgacgaggcc
     3721 cacatcccag gcgccgtcta cgttgactgg accagcgaca tcaccgaccc cgacgatccc
     3781 gtgccggtgc agatcgcacc gccagaacgt ttcgctgatc ttatgggatc tctcggcatc
     3841 ggcgacgata ctagcgtcgt ggtatacgac cacgcgggtg gccagttcgc cacgcgcctc
     3901 tggtgggccc ttacgtacta cggccacgac ggggtcttcg tcctcgacgg cggctggagg
     3961 aagtggacgg ccgaggaccg tccgaccacc gccgaagtcc ccgaaccgga gccggccgtc
     4021 ttcacccccg aacctcagag cggctggcgg acggacgcgg aaggtctcct cgccacgagc
     4081 gatggtggcg acgctctcgt tctcgatgcg cgcgacgagg ggcagtacac cggcgccgtg
     4141 acgcgagagg aggccaggac ggggcgcgtg ccaggggccg cccacctcca ctccgacagc
     4201 ctcttcgacc ccgagagcgg caccttccac tccgacgagg agctggcgcg gaggctggag
     4261 gaggcgggtg tgtcagggga tagagacgag cccgtcgtcg cctactgcaa cggcggggtc
     4321 gcggccacgg tcccgctctt tgccttgcac cggctgggtt acaagagact ggccaactac
     4381 gacggctcgt ggaacgaatg gggcaacagg gaggatctgc ccgcagagcg ttgaggggac
     4441 gaccagagag cggggagcga cttcgatgtt agaatctccg gcggcatgaa aacgtacaac
     4501 gtccacaggt cgcgggtaag aaacatcgtt tcctgggtgc tgagcctgct aatgatagcg
     4561 gccggcgtcg cgcttatcgc ctccttcttc ctggccggac gcctggacag taccgccacg
     4621 aacagcgaca accccggcgg tttcaacgtg cccaagctgg agaccacgga cgaagagaac
     4681 acgagcagca ccggcccaag cgacaagacc ctcgagctta ccgtccccgc gatggccagg
     4741 atcgagaacg acgaagtacc ctacacggcg gggaacgacg aggaggcgct gaagacccac
     4801 gccgccatcc acctcgaagg caccggcttt ccctgggata acgaggccaa cgtctatatc
     4861 gcgggccaca ggctcggcta cccaaacacc gagagctggc tgaccttctg ggacctgagc
     4921 aacgtcgtgg gcggcgacaa gatctatgtc accgacgcta acggtaccga gtacacctac
     4981 gacgtcttca agaccttcgt cgtcagccct tctgctaccc aggttacgga taccctgccg
     5041 ggcaagaaca tactgacgct ccagtcatgc acgctgcccg attacggcaa gcgtctgatc
     5101 gtacaggccg aacttaccga gacatcggag aaagcggcgt aggatcccac gccgtggacg
     5161 agggactcct cgaagggcag ctggcctact accgggcacg cgccggggag tacgacgaat
     5221 ggtttctgcg cacgggacgt cacgaccgcg gtcccgaatg gaatcgcacg tggtttctcg
     5281 agctggagcg agtccgccag gaactcgacc ggttcgggcc gacgggaagg gtgctcgagc
     5341 tcgcgtgcgg caccggtctg tggacagtgg agctcgcgag gcacgctgca ggcataaccg
     5401 ccgtcgacgc ctcgccagag gtactggata tcaaccgggc cagactgcaa gagacccttc
     5461 ccgaggcgac cgtcagctac gtcagggccg acctgttcgg ctggaggccc gaagagtctt
     5521 acgacgtcgt gttcttcggc ttctggctct cgcacgtgcc tccagggcgt ttcgccgcct
     5581 tctgggatct cgtccggtcg gcgctacgac caggcggacg cgtcttcttc gttgactcgc
     5641 tgcgcacgga aacatgggcc gagaaggaga gcgccgggcg gggccccagg gaccacacga
     5701 cgctcagacg actcaacgac gggcgcgagt tcagtatcgt caaggtcttc tacgaccctg
     5761 tagagctcga agcgcgtctc gccggcatgg gctggcgcat ctcggtgcgc acgacggaga
     5821 aacacctgct ctatggtttc ggcgagtaca aaaggtaatc cgcaggaact cgtttcgcga
     5881 tgtacgatct ccttggcatt tccgtcattc ccgcgcaagc gggaatccac gcgctccccc
     5941 aaccttcgca gacgctacgt ccc
//
LOCUS       DATMVF010000295         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_591275, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000295 DATMVF010000000
VERSION     DATMVF010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591275"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /gene="rfbB"
                     /locus_tag="VN178_15485"
     CDS             <1..886
                     /gene="rfbB"
                     /locus_tag="VN178_15485"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWS82419.1"
                     /translation="GDRGLVDRLLAEYRPSAVVNFAAETHVDRSIDGPKAFVQTNVVE
                     THELIEAALSYWSILEGEDHDAFRFLHVSTDEVYGTLGPEGCFTEESPFRPNSPYAAS
                     KAASDHLVRAYHHTYGLPTLTTNCSNNYGPYHFPEKLIPLMILTALRGEPLPVYGDGQ
                     HIRDWLYVEDHCKAIVVVLQRGRPGETYNVGGHNERTNLEVVKTICALLDGLVPGSPH
                     ESLITFVADRPGHDRRYAIDAGKIERELAWRPEETLETGLEKTVRWYLENRGWCEAAL
                     SRGYGGERLGLGSRGGPA"
     gene            883..1764
                     /gene="rfbA"
                     /locus_tag="VN178_15490"
     CDS             883..1764
                     /gene="rfbA"
                     /locus_tag="VN178_15490"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HWS82420.1"
                     /translation="MKGIVLAGGSGTRLYPLTRAVSKQLLPVYDKPMIYYPLTTLMLA
                     GIKDMLVISTPEDLPRFEDLLGDGSQWGVDFSYAVQPRPEGLAQAFVIGREFVGSESV
                     SLVLGDNIFFGHGLSELLERSAGRQRGATVFGYYVREPGRYGVVEFDADGTVLTIEEK
                     PESPRSHYAVTGLYFYDNRVLDVAADLTPSARGEFEITDVNVDYLERGELQVELMGRG
                     MAWLDAGTHESLHQSSNFIETIEQRQGLKIGCPEEISYRKGYIDAESLERLAYPMRKN
                     DYGRYLLELLKSEEYVT"
     gene            1761..2321
                     /gene="rfbC"
                     /locus_tag="VN178_15495"
     CDS             1761..2321
                     /gene="rfbC"
                     /locus_tag="VN178_15495"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWS82421.1"
                     /translation="MNVLRTELPGVLVIEPDVFGDARGFFMESWNGARYEEHGVPDRF
                     VQDNLSFSAYGVLRGLHFQNPKAQGKLVSVLLGEVFDVAVDIRVGSPTFGGWTGSTLS
                     EENRRQLWVPPGFAHGFVVTGENALFSYKCTDYFAPEYDHSILWDDPAIGIQWPIQTP
                     SLSDKDRAAPPLNRVPEDTLPYYKAR"
     gene            2684..3367
                     /locus_tag="VN178_15500"
     CDS             2684..3367
                     /locus_tag="VN178_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82422.1"
                     /translation="MAARLLLLLVVMVIFAALYGCAQEAAKDRQPVKRVKEPTIAEIA
                     PHAPEESDDQVRRAKKAGVVVRAGNSAVVTQAGEAVVRVSGFRTAKQEGADATAPSRE
                     VTLRIEGSPGTEFSGTCIIGGKAEKKVSGRVPERFIYELDGQPLECEISKQSSNPGKM
                     MVEFSAGGHTNSVQQTSTQGSSLILRYDGHGSTALVSSSTSVSGNQANSTSQVSVLQS
                     NAASGRDSH"
     gene            complement(3406..4245)
                     /locus_tag="VN178_15505"
     CDS             complement(3406..4245)
                     /locus_tag="VN178_15505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWS82423.1"
                     /translation="MKFSVIIVNYASWPLALRCIESLRRTRCGQFEVFVVDNDSVEPP
                     ELPSWVRLIRNKENVGFARAHNRGIAASSGDPVVLINPDTLVERDFFEQLEAFVSENP
                     KAGIFGPRIVDSEGRLQLSARREVSALSGFLGRTSLLTRLFPKSSIVRSQFPAVTDQS
                     RPTPVDWVSGACMVVRRDTLRDVGHLDERFFMYFEDADLCRRARAAGWLVYYLPQVEI
                     VHQTGASSRSKPKAIWLLHKSAFLYHRKHGVHGPLGAYSAMVLAGLTLRALAKLGISM
                     FAR"
     gene            complement(4242..5246)
                     /locus_tag="VN178_15510"
     CDS             complement(4242..5246)
                     /locus_tag="VN178_15510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWS82424.1"
                     /translation="MPPRATVVIPNWNGRQFLGLCLGSLREQTWGAFQTILVDNGSVD
                     GSATFVKEHFPEVDVVSLGENRGIAAAFNAGIGASTAEYVVLLNNDTEQDPGWLEALV
                     RAAEDHPEAGFLASKLVDFHDRTMLDGAGDAMRLSGLPYRLGHGERDGCRFDRPGYVF
                     GACAAAALYRRDMLDEVGLFDEDFLSYCEDGDLSFRAQLAGYRCFYVPGAVVYHMGSA
                     STGGKRSPTATRLGSRNSFSLLVKNLPLSAVPHVLPFFAAGQLGRMITAAVTGSLRAH
                     LEGLAGAWRHLPLMLAKRAEIQKRKKVSDAEVRRLLRESSLAATASIARRLRDRVLSR
                     "
     gene            complement(5251..>5776)
                     /locus_tag="VN178_15515"
     CDS             complement(5251..>5776)
                     /locus_tag="VN178_15515"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HWS82425.1"
                     /translation="IEHEEEDDAPARNDSLGTPNIVGGAEILGIELLDESGQPAGALP
                     SGATLTVRVHLRYDETMEVSTLGIALRSERAGLEVFSTDTALEGTPLGRKDKGEQATV
                     DFTFVMPFGPGIYKVDASLTDPQAEDSDVVLTRDATTLEITREGALAPVRGLVRLPAR
                     VEIHTPEGRQEGPA"
BASE COUNT         1100 a         1829 c         1823 g         1024 t
ORIGIN      
        1 aggggaccgc gggcttgtcg accgcctgct cgctgagtat cggccctcgg ccgtcgtcaa
       61 cttcgccgcc gagacgcacg tcgaccgctc catcgacggt ccgaaggcct ttgtacagac
      121 caacgtggtc gagactcacg aactcatcga ggccgcgctc tcctactgga gcatcctcga
      181 aggggaggac cacgacgcct tccgctttct gcacgtctcc acggacgagg tgtacgggac
      241 gctgggaccc gagggctgct ttaccgagga gagtcctttc aggcccaact ccccctacgc
      301 cgcctccaag gcggcctccg accatctcgt gcgggcttat caccacactt acgggctccc
      361 cacgctcacg accaactgct ccaacaacta cgggccctac cactttcccg agaagctcat
      421 accgctcatg atcctgaccg ccctgcgagg agaacctctg cccgtctacg gcgacgggca
      481 gcacatcaga gactggctct acgtagagga ccactgcaag gctatcgtcg tcgtcctcca
      541 gcgtggccgt ccgggcgaga cctacaacgt cggcggccac aacgagcgga cgaacctgga
      601 ggtcgtcaag accatctgcg ccctgctcga cgggctggtt cctggatcgc cgcacgagtc
      661 gctcatcacg ttcgttgcgg acaggcctgg ccacgacagg cgctatgcca tagacgcggg
      721 caagatagaa cgcgagctcg cctggaggcc cgaggagacg ctggagacgg gcctggagaa
      781 gaccgtgcgc tggtacctgg agaaccgcgg gtggtgcgag gccgcgctct cgcgcggcta
      841 cgggggagag cggctggggt tgggcagtcg cggagggccg gcgtgaaggg catcgtgctc
      901 gccgggggct cgggtacgcg actgtatccg ctcacgaggg ccgtaagcaa gcaactcctt
      961 cccgtctacg acaagccgat gatctactac ccattgacca cgttgatgct cgctggcatc
     1021 aaggacatgc tcgtgatctc cacccccgaa gaccttcccc gcttcgagga tctactcggc
     1081 gacggttcgc agtggggtgt agatttcagc tacgccgtgc agcccaggcc cgaaggcctg
     1141 gcccaggcct tcgtcatcgg gcgggagttc gtcgggtccg agagtgtctc cctcgtcctc
     1201 ggggacaaca tcttcttcgg gcacggtctc tcggagctcc tggagcgttc ggcgggcagg
     1261 cagagaggtg ccaccgtctt cgggtactac gtcagggaac cgggtcgcta cggcgtcgta
     1321 gagttcgacg cagacgggac ggtgctcact atcgaggaga agccggagag cccccgctcg
     1381 cactacgccg tcacaggcct ctacttctac gacaacaggg tgctcgacgt ggcggccgac
     1441 cttacacctt cggcgcgggg agagttcgag ataacagacg tcaacgtcga ctatctcgag
     1501 cgcggggagt tgcaggtcga gctcatgggc cgcgggatgg cgtggctcga cgccggcacg
     1561 cacgagtccc tgcaccagtc gtccaacttt atcgagacca tcgagcagcg ccaaggcctc
     1621 aagatcgggt gtcccgagga gatctcctac cgcaaaggct acatcgacgc cgaaagtctc
     1681 gaacggctcg cctatccgat gaggaagaac gactacggcc ggtacctgct cgagctactg
     1741 aagagcgagg agtacgtcac ttgaacgtcc tccggacgga actgccagga gtactcgtca
     1801 tcgagcctga cgtcttcgga gacgcccgag gtttctttat ggagagctgg aacggcgccc
     1861 gctacgaaga gcacggcgta ccggaccgct ttgtgcagga caacctctcg ttctcggcct
     1921 acggtgtgct tcgtggcctc cacttccaga accccaaagc ccagggcaag ctcgtctccg
     1981 tcttgctggg cgaggtcttc gacgtcgccg tagatatcag ggtcggctcc ccgactttcg
     2041 gcgggtggac gggctcgacc ctctcagaag agaacaggcg ccagctctgg gttcctcccg
     2101 gcttcgccca cggtttcgtc gtcacgggcg agaacgctct cttctcatac aaatgcacag
     2161 actacttcgc tcccgagtac gaccactcca tcctgtggga cgaccccgca atagggatac
     2221 agtggcccat ccagacccct agtctctccg acaaagatcg cgcagcccca ccgctgaacc
     2281 gggtgccgga agacaccctt ccttactaca aggcacgctg agacgggcga accggtccct
     2341 tcgaggtgct cgaggacaat tgctacacgc gcataattac ctacggcaag ggcaccgcgg
     2401 cggtgggccg ctgagcgaat tacagcgccg tgcaaaaggg ttcaaccctt atgcacgacg
     2461 agctatcagc caaaaacaaa aagctgatcg ctgatggctg atagctgacg gcgcgggttg
     2521 cagaagaatt gaccttctgc aacccgctgt acgcggcaag cagtgttcag caagtcgttg
     2581 cgcatcagtg atgctgtagg taccttctag gtaggcactc cggtgaatag ggacttgtag
     2641 gatcgggtac gaaagttcag aggacacaag gagaggaggg agcatggcgg cacgtctcct
     2701 cctgttgttg gtggtcatgg taatcttcgc ggccctctac ggttgtgccc aggaggctgc
     2761 aaaagaccgg cagccagtga agagggtaaa ggaacctacc atagccgaga tcgctccgca
     2821 cgcgccggaa gaatcggacg accaggtacg gcgggcgaag aaggcgggtg tcgtggtgcg
     2881 cgctggaaac tccgcagtag taacacaggc tggggaggct gtggtgcgag tgtcaggttt
     2941 cagaaccgcg aagcaagagg gtgccgacgc tactgccccc tccagggagg tcacgctgag
     3001 gatcgagggt agcccaggaa ccgagttttc cggcacgtgt atcatcgggg gcaaagcgga
     3061 gaagaaagtc agcggacgag tcccagagcg gttcatctac gaactagacg ggcagcccct
     3121 cgaatgcgag ataagcaagc agagctcgaa ccctgggaag atgatggtcg agttcagcgc
     3181 cggaggtcac acgaactccg tccagcagac cagcacccaa ggcagctctc taatcctccg
     3241 gtacgacggc catgggagca ccgctctcgt ctcatcgtcc acttctgttt cgggcaatca
     3301 agcgaactcc acctcgcaag tgagcgtctt gcagagcaac gcggcctctg ggcgagattc
     3361 tcactaggcc gccttccaac ttcacgagca gatttagcct gacgcctatc tcgcgaacat
     3421 cgagatcccc agcttcgcca gggcgcggag ggtgagtccg gcgagaacca tcgcgctgta
     3481 ggcgccgagg gggccgtgga cgccgtgttt gcggtggtag aggaacgcgc tcttgtggag
     3541 gagccagatc gccttcggtt tacttcggct gcttgcgccg gtctggtgga ctatttcgac
     3601 ttgcggcaga taatagacga gccagcctgc cgcgcgggcg cgcctgcaga ggtcggcgtc
     3661 ctcgaagtac atgaaaaagc gttcgtccag atggcctacg tctcgcagtg tatccctgcg
     3721 gacgaccatg caggcgccgg agacccagtc gaccggggta gggcgggact ggtccgtgac
     3781 ggcggggaac tggctcctta cgatagagct cttggggaag aggcgggtca ggagggaggt
     3841 gcgtccgagg aaccctgaga gggcgctgac ctcccgcctt gccgagagct ggagacggcc
     3901 ctccgagtcc actatcctcg gcccaaagat accggctttc gggttctctg agacgaatgc
     3961 ttccagctgc tcgaagaagt ccctctccac cagggtgtcg gggtttatga ggactacggg
     4021 atctccgctc gatgcggcga tgcccctgtt gtgggcgcgc gcgaaaccta cgttctcttt
     4081 gttccggatc aggcgcaccc aggatggaag ctcgggaggc tccacgctgt cgttgtcgac
     4141 gacgaagacc tcgaactgtc cgcaacgcgt ccgtcgcagg gactcgatgc agcgcaacgc
     4201 caggggccag gaggcgtagt tgacgataat gacggaaaac ttcatcgcga caagacccta
     4261 tctcgcagcc ggcgcgcgat gctcgccgtc gcggcgagcg atgactccct gaggagcctg
     4321 cggacctcgg cgtcggagac cttcttcctc ttctgtatct cggcccgctt ggcgagcatg
     4381 agcggcaggt ggcgccacgc gcccgcgagt ccctccaggt gcgcccgcaa agagcccgtc
     4441 acggcggcgg tgatcatgcg cccgagttgt cccgcggcga agaaaggtag tacgtgcggt
     4501 accgcggaga ggggcaggtt cttgaccagc aggctaaaag agtttcggct accgagcctg
     4561 gtcgccgtcg ggctacgctt gccgccggtc gaggcgcttc ccatgtggta gacgacggcg
     4621 ccggggacat agaagcaacg ataaccggcg agctgggcgc ggaagctcag gtccccatcc
     4681 tcgcagtagg agaggaagtc ctcgtcgaag agcccgacct catcgagcat gtccctgcgg
     4741 tagagcgccg ccgccgcaca cgccccgaag acgtagccgg gcctgtcgaa cctgcacccg
     4801 tcgcgttcgc cgtgcccgag gcggtaaggt agaccgctca gacgcatggc gtcccctgcc
     4861 ccgtcgagca tcgtgcggtc gtggaagtcg accagcttgc tcgcaagaaa gccggcctcg
     4921 ggatgatcct cggcggcgcg caccagcgct tccagccagc ctgggtcctg ctcggtgtcg
     4981 ttgttgagca gtaccacgta ctccgccgta gacgccccga tccctgcgtt gaaagccgcg
     5041 gcgatgcccc gattttcgcc gagcgagacc acgtcaacct cagggaagtg ttcctttacg
     5101 aaggttgccg atccgtcgac ggagccgtta tccaccagga tcgtttggaa ggcaccccac
     5161 gtttgctctc gcagagagcc aaggcacagc cccaggaatt gcctgccgtt ccagttcggg
     5221 atgacgaccg tggcgcgggg aggcacggct tcaggctggc ccctcctgcc ggccctccgg
     5281 agtgtggatc tcgactctcg ccggcagtcg taccaggcct cttaccgggg cgagagcccc
     5341 ttccctggtt atctccaagg tcgtcgcgtc ccttgtgaga accacatccg agtcttcagc
     5401 ctgagggtcc gtcagggaag cgtccacctt gtagatgcct ggtccgaacg gcatcacgaa
     5461 cgtaaagtcc acggtagcct gctcgccctt gtccttccgc ccgagcggag taccttcgag
     5521 cgccgtatcc gtcgagaaga cctcgaggcc ggccctttcg ctccgcagtg caatgccaag
     5581 ggtactgacc tccatggtct cgtcgtagcg caggtgcacc cgcacggtca gggtcgcgcc
     5641 cgagggcagc gcgccggccg gttgcccgct ctcgtcgagc agttcgatgc caaggatctc
     5701 ggcccctccc acgatgttcg gggtcccgag gctgtcgttc ctggccggcg catcatcctc
     5761 ttcttcgtgc tcgatc
//
LOCUS       DATMVF010000296         5505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_640787, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000296 DATMVF010000000
VERSION     DATMVF010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640787"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2304
                     /locus_tag="VN178_15520"
     CDS             <1..2304
                     /locus_tag="VN178_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HWS82426.1"
                     /translation="VLSLMERYDDFVFNQSSAQLYRWIETESPEIFERVRRRVEEGRW
                     EPVGGSWVEADCQIPSGESMVRQMLYGQRYFEDKFGHRSRVAWLPDAFGFSPALPQLL
                     RGAGMEGFFTYKLNWSETNEFPYDLYEWEGIDGSTVIAHDFENPGQDYNGNITPRDIY
                     GTWRNFEGKRHHPESLLSFGWGDGGGGPSEQMLENYARMKSFPAMPRLRMAHVDEFFD
                     RLPKQGLPRWTGELYLELHRGTLTTQARVKKLNREAEHRLLEAEALATLAALHGGEYP
                     REELEAAWKTLLLNQFHDILPGTSIGEVYEVTHKELAGVVGTAQELRDRALSPPQQAE
                     SALLVANVSLNPRPLSVVLDAESRPEGTVRVAGTEGEPLPTQETECGGLLVHAPDLSV
                     PGLGWRTLAAIHESAPARREARDVGAEGAEGRAVLDNGILRVEVGEDGTLHGVYDREV
                     GREVLAGRANQLWAYTDKPRNWEAWDVDEGYEQEGEEISSVQSVEVVENGPLRASVRV
                     VRRWRDSTIAQTYRLLSGSRRLDVESYVDWRERRVLLRTLFPLAVRSHETTSETMFGA
                     LRRPTHHNTPWDATRFEVSAHRFCDLSEPGYGVALLNDGKYGHSAKWNVVGISLLRSP
                     LYPDPFADEGEHHFTYSLFPHSGDWTASGVAREAFALNSPLFPVMAPKDTLPQGFSLV
                     EVEGLELALGSLKLAEEGDTVLLRLYEPHGARGECTLRFGRPTERVERVNLLEEAEGP
                     VEVQGSAARLRLRPFEVVTLRVEWERE"
     gene            2328..3200
                     /locus_tag="VN178_15525"
     CDS             2328..3200
                     /locus_tag="VN178_15525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWS82427.1"
                     /translation="MEHRRLGRLGSENSVLIFGGAALAEATEEDSDTAIAQALDAGVD
                     HFDTAADYGDSELQYGRWMPEIRDRIFLSTKTGLREKDAAKRQIQDSLDRLRVDDVDL
                     LQLHSVGDIEDLDRATGPGGSLEAAVQAKEEGLIGAIGITGHGNEAPATHLEALRRYP
                     FETVLTPWNYILSTDEGYRADYEALVEEVRRQDAGLMIIKTISRRNWPEGGASGHRYT
                     TWYEPFDQQEYIDAAVSWVLSHEEVTGLAMAGDVTIVPMILKTGGQKMSREDAEQILS
                     RAPDYSSPFISMPI"
     gene            3556..5178
                     /locus_tag="VN178_15530"
     CDS             3556..5178
                     /locus_tag="VN178_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWS82428.1"
                     /translation="MGNLALLQSLVEVNETWLDYFIIGVYFVFVLGIGAALRTKMQTS
                     EDYFLSGRSLPSWITGLAFLGANLGALEIMGMGAGAATYGIMQAHYYWIGAIPAMVFV
                     ALFMIPFYYASRVHSVPGFLKLRYNEATRGFNAILFAIFMLLLSGINMFAMALVFQLL
                     LGWSLTASIFLSAGIVLAYVVLGGLSSSIYNEVLQFFLITLGLVPLVIFGLIDVGGLA
                     GLKESISNPQFFHTWANTGTKDNPFAVQWFYIVFGLGLVQSFGYWCTDFLVVQRALAA
                     EDLAASQRTPLIAAFPKLLYGVLAIFPGLIALSVLGPKIATAAEANNAVPYAMAYYFP
                     TGMLGFGLTALLAAFMSGMAGNVTAFNTVWTYDIYKAYINRDATDRHYLNVGRIATVA
                     GVLLSVVTAYLALGFETIINYVQVLHGIFLAPLFGTFLLGMFWRRTTGWGGFSGLVAG
                     TGYGIITYLITLDSGLGASMARAFFAWIVCLAVTIAVSLVTQPKPDSELRGLVWGLTE
                     QKEAVEEVWYKKPWVLAVTVLTITIALNIYFW"
     gene            5188..5490
                     /locus_tag="VN178_15535"
     CDS             5188..5490
                     /locus_tag="VN178_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82429.1"
                     /translation="MTEEHQSEERHHEYGEEERVPEHSGTNHSMAIGFYIAALVLIYG
                     VTMVLVGLLSPAAPSEGDLGFNVNFWWGLVMVVSGCVILGMTLASPRRRAARNQQQ"
BASE COUNT         1029 a         1753 c         1737 g          986 t
ORIGIN      
        1 gtcctctcgc tcatggagcg ctacgacgat ttcgtcttca accagtcctc ggcccagctc
       61 tacaggtgga tagagaccga atcgccggag atcttcgagc gggtaaggag gcgggtagag
      121 gagggacgct gggagccagt aggcggctcg tgggtcgagg ctgactgcca gatcccatcc
      181 ggagagtcca tggtgcgcca gatgctctac ggccagaggt acttcgaaga caaattcggc
      241 catcgctcgc gggtggcctg gctccccgac gccttcggat tctctcctgc gctgccgcaa
      301 cttctgcggg gcgccgggat ggagggcttc ttcacctaca agctcaactg gagcgagacc
      361 aacgagttcc cgtacgacct ctacgagtgg gaaggcatag acggtagcac ggtgatcgcc
      421 cacgacttcg aaaaccccgg ccaggactac aacggcaaca ttacaccccg cgacatctac
      481 ggcacctggc gcaacttcga gggcaagcgc caccaccccg agagcctcct atccttcggg
      541 tggggcgacg gcggaggcgg cccgagcgag cagatgctcg aaaactacgc ccgcatgaag
      601 agcttccccg ccatgccgcg cctccgcatg gcccacgtcg acgagttctt cgataggctc
      661 ccgaaacaag gcctcccaag gtggaccggc gagctctacc tcgagttgca caggggcacc
      721 ctgaccaccc aggccagggt caagaagcta aaccgcgagg ccgagcaccg tctgctcgag
      781 gctgaagccc tcgccacgct ggccgcgctc cacggcggcg agtacccgcg cgaagagctc
      841 gaagcggcct ggaagacgct cctcctcaac cagttccacg acatactgcc agggacctcg
      901 ataggggagg tctacgaggt aacccacaaa gagctagcag gcgtcgtcgg gacagcgcaa
      961 gagctgcgcg acagagccct gagtcccccc cagcaagcag agtctgcgct cctcgtcgcc
     1021 aacgtctcac tgaacccacg tcccctctcc gtcgtactcg acgccgagag caggccggaa
     1081 ggcactgtaa gagtcgccgg aactgagggc gaacccctgc ccacccagga gaccgagtgc
     1141 gggggactgc tcgtgcacgc cccggatctc agcgtgcctg gactcggctg gcgtacccta
     1201 gcggccatac acgagtccgc gccggcccgg cgcgaggcca gggatgtcgg tgccgagggc
     1261 gcggaagggc gcgccgttct ggacaacggt atcttgcgtg tggaggtagg ggaggacggc
     1321 acgctccacg gcgtctacga cagggaggta ggccgtgagg tactggccgg acgcgccaac
     1381 cagctctggg cctacacgga caagccccgc aactgggagg cgtgggacgt ggacgaggga
     1441 tacgagcaag aaggggagga gatctcctct gtgcagagtg tcgaggtcgt cgagaacggg
     1501 ccgctcaggg cttccgtgcg cgtggtccgc aggtggcggg actcgaccat cgcgcagacc
     1561 tacaggctcc tctccggctc gcgccgcctg gacgtggagt cttacgtcga ctggcgcgag
     1621 aggagggtcc tcctcaggac gctcttcccg ctcgccgtcc gctcccacga gactaccagc
     1681 gagacgatgt ttggggccct gcggcggccg acgcaccaca acacaccctg ggacgccacc
     1741 cgtttcgagg tctccgccca ccgcttctgt gatctctccg agccgggcta cggtgtggcg
     1801 ctcctcaacg acggcaagta cggccacagc gcgaagtgga acgtagtggg catcagcctg
     1861 ctccgcagcc cgctctaccc cgacccgttc gccgacgagg gcgagcacca cttcacctac
     1921 tccctcttcc ctcactcggg cgactggacc gcgtcggggg tggcgcgcga agcattcgcc
     1981 ctaaactcgc cacttttccc tgtgatggcg ccaaaagata ctctgccgca gggattctct
     2041 ctcgtcgagg tagagggact ggagctcgcg ctcgggagcc tcaagttggc ggaggaagga
     2101 gacactgtgc tcctgcgcct ctacgaaccc cacggcgccc gcggggagtg tacgttacgc
     2161 ttcggtcgcc ccaccgagag ggtggagcgc gtcaaccttc tcgaagaggc cgaagggccc
     2221 gtggaagtac aaggaagtgc cgcgaggctc aggttgcggc cgttcgaggt ggttacgctg
     2281 agagtggagt gggaaagaga ataaggtaag cgaggaaagg gccagggatg gaacaccgtc
     2341 gtttgggacg tcttggcagc gagaactccg tgctcatctt cggcggggcc gcgcttgcgg
     2401 aggccaccga ggaagactcg gacacggcca ttgcacaggc gctcgacgcc ggcgtggacc
     2461 acttcgatac ggcggccgac tatggcgact cagagctaca gtacgggcgc tggatgcccg
     2521 agatcaggga ccgcatcttc ctctccacca agaccggcct gcgcgagaaa gatgcggcca
     2581 aacgccagat ccaggactcc ctggatcgcc tgcgcgtgga cgacgtcgac ctgcttcaac
     2641 tccactccgt gggcgacatc gaagatctcg accgcgcgac gggtcccggc ggctccctcg
     2701 aagccgccgt ccaggcgaaa gaagagggcc tcatcggcgc catcggcatc acgggccacg
     2761 gcaacgaggc cccggcgacg cacctcgaag ccctgcggcg ttacccgttc gagacggtgc
     2821 tcacgccctg gaactacatc ctttccaccg acgagggcta tcgggccgac tacgaggcgc
     2881 tcgtggaaga ggtgcggcgc caggatgccg ggctcatgat catcaagacc atctcgcgcc
     2941 gtaactggcc cgagggcggc gcgagcgggc acaggtacac cacctggtac gagcccttcg
     3001 accagcagga gtacatagac gccgccgtct cctgggtgct ctcccacgag gaagtcaccg
     3061 gactggcgat ggccggcgac gtcactatcg tgccgatgat cctgaagacc ggtggacaaa
     3121 agatgtcgcg cgaggacgcc gaacagatcc tctcccgcgc ccccgactac tcctcgccgt
     3181 ttatctccat gccgatctaa cagcgccctg gtcgaggctc gagcctggac caccgcgcgc
     3241 tatcaacctt cagcctcttt cttgttgaca gcgcggcttg cggaaggact gaacttccgc
     3301 aagccgccat ggtttgacac ggataaaccg agggtattaa cgtctcatta cagggaaata
     3361 agaggagggt atggcgttac tgctgtcgaa ggtatcggtc gacaacccgt ggctgaatta
     3421 cccgatcatc gacgttgacc ctaccgagaa tggattacgg gatctgctgg gaggaactct
     3481 gacagaacct tgacaacaag gggtggtaga ggggatcgcg gtcggggaac cgggatatga
     3541 agggagaagg ttacggtggg aaatctggcg ttgttacaat ctctggtcga ggtcaacgaa
     3601 acgtggctcg actacttcat tatcggggtg tacttcgtat tcgtgctcgg catcggggct
     3661 gcgctcagga cgaagatgca gacctccgag gactacttcc tctccggaag gtcactgccg
     3721 agctggatca cgggcctcgc cttccttggt gcgaacctgg gggcgttgga gatcatgggg
     3781 atgggcgcgg gggcggcgac gtacgggatc atgcaggccc actactactg gatcggcgcg
     3841 atcccggcga tggtcttcgt ggccctgttc atgatcccct tctactacgc cagtcgcgtc
     3901 cactccgtcc ccggcttcct gaagctccgc tacaacgagg cgacgcgtgg gttcaacgcc
     3961 atcctgttcg ccatcttcat gctcctgctc tcgggcataa acatgttcgc gatggcgctc
     4021 gtcttccagc tgctccttgg ctggtcgctc acggcgagca tcttcctctc tgcgggaatc
     4081 gtactcgcgt acgtggtctt gggcggcctc tcctcctcga tctacaacga ggtcttgcag
     4141 ttcttcctga tcacgttagg tttggtgccg ctcgtgatct tcggccttat agacgttggc
     4201 ggtctcgccg gtttgaagga gtccataagc aacccccagt tcttccacac ctgggccaat
     4261 acgggcacga aggacaaccc ttttgccgtg cagtggttct acatcgtttt cgggctcggt
     4321 ctggtgcaga gcttcggcta ctggtgcacg gacttcctcg tggtgcagag ggccctggcg
     4381 gccgaggatc tggcggcatc ccagaggacc ccgctcatcg cggcgttccc gaaactcttg
     4441 tacggggtac tcgcgatctt ccccgggctg atcgccttgt ccgtcctggg cccgaagatc
     4501 gccaccgcgg ccgaggccaa caatgccgtc ccctacgcga tggcctacta cttccccacc
     4561 gggatgctcg ggtttggtct gacggcgctg ctcgcagcct tcatgagcgg gatggccggc
     4621 aacgtgacgg ccttcaatac cgtctggacc tacgacatct acaaggcgta catcaaccgt
     4681 gacgccaccg accgccatta tctcaatgtg ggccgcatcg ccaccgttgc cggggtgctc
     4741 ctgagcgttg taacggccta cctggctctc ggcttcgaga ccatcataaa ctacgtccag
     4801 gtgctgcacg gcatattcct ggccccgctc ttcgggacgt tccttcttgg catgttctgg
     4861 aggcgcacga cgggctgggg tggattcagc gggctcgtcg ctggaacggg ttacggcatc
     4921 atcacgtacc tcatcacgct ggacagcggc ctgggggcgt ccatggcgcg agcgtttttc
     4981 gcgtggatcg tgtgcctcgc cgtgacgatc gcggtctctc tggtcaccca acccaagccg
     5041 gactccgaac tgcgcggctt ggtgtggggc ctgaccgagc agaaggaggc cgtcgaggag
     5101 gtctggtaca agaaaccgtg ggtcctcgcg gtcaccgtgc tcaccattac cattgccctg
     5161 aacatctact tctggtagga ggagagcatg acagaggaac accagagtga agaacgacac
     5221 catgaatacg gggaagagga gagggtgccg gagcactcag gcacgaacca ctccatggcg
     5281 ataggcttct acatcgcggc cctcgtcctt atctacggcg tcacgatggt cctggtcggg
     5341 cttctctccc ccgccgctcc gagcgagggc gacctcggct ttaacgtgaa cttctggtgg
     5401 ggtctggtca tggtcgtttc gggatgcgtc atcctgggta tgaccctcgc ctcgccacgc
     5461 cgccgcgccg cccggaacca gcagcaatag tgtaggggcg ggttt
//
LOCUS       DATMVF010000297        23909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_264852, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000297 DATMVF010000000
VERSION     DATMVF010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 23909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23909
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264852"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VN178_15540"
     CDS             complement(<1..586)
                     /locus_tag="VN178_15540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWS82430.1"
                     /translation="MSLTFYIVDVFAEERYAGNQLAVVHGGADLPDETLQKIALEMNY
                     SETTFVLSDEESEGGYDVRIFTPGEEVPFAGHPTLGTAYVIQHELLAGPVGGITLNLK
                     AGEIPVTFGEVLWMRQLPPTFGAVLDHASVARALNLEAADLNDHYPVQEVSTGLPAIV
                     VPLRDLDALRRCKVDWERYTEASGPGNNLYVFCPE"
     gene            complement(787..2226)
                     /locus_tag="VN178_15545"
     CDS             complement(787..2226)
                     /locus_tag="VN178_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWS82431.1"
                     /translation="MSGAGVAGASLLAGCERAGVLPDLPGNTPAADRDTNVVLIILDS
                     LRKDHVGAYGNEEIRTPNLDALARESLLFSRAYPESAPTICARRAIHTGLRTWPFEDW
                     RRYKGIDVGLQGWQPIPNYQITLAETVRSAGYETLFVTDNLQQYDASMNFHRGFEAFD
                     FIRGQTTDSYRPLYTCPPGKVEQALLNRAVLGTGSRAYFQRYFANTAYRRSEEDWFAP
                     QVFTRASEFLEAASERPFFLTVDCYDPHAPWDPPAEYVAMYDEGYEGPEPFAPVDGAT
                     DYLSGRQIRRMSALYSGEVTMTDRWLGRFLDKMEELDLFDNTLLVLLSDHGIAHGEHG
                     VVGKLPSSLWPEVTDIPFLIRHPEGKGAGKTSDYYASTHDVAPTVLGFLGIEPPQPLD
                     GQDLSVMLRGGEPEARSHFTLGYHDHVFTRDEEYAMISRNDGTEARLYDLREDPGMYE
                     DLAGDLPAVVRRMFSNYVLEDAGGPLPRY"
     gene            2404..2652
                     /locus_tag="VN178_15550"
     CDS             2404..2652
                     /locus_tag="VN178_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82432.1"
                     /translation="MAAFRYGREHFEEGEYVRRVEATPGDGLDPVVRIVEDLGTVEIP
                     CGKVDCRSNCLEYLVSATGSRNDYTLAECKISALVSII"
     gene            complement(2813..3394)
                     /locus_tag="VN178_15555"
     CDS             complement(2813..3394)
                     /locus_tag="VN178_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82433.1"
                     /translation="MFHWRHGGWTVILLRLLGIGAVYGVGFMFIKSLFPEPFVLLILQ
                     GGRSSEGDLTTLALVYMGVGLLAGLIAGPIFGGALLLRRGRSERSEGSGPRFVLSLAL
                     ALLTGFISGILTLIVYATGILPSGGVLDPLRLIEGANHPPGAPLLVAWTIARDLLPAG
                     LAGLFLAPLAGGALQRLYVAERPPEQKRYSWEE"
     gene            3510..4670
                     /gene="queG"
                     /locus_tag="VN178_15560"
     CDS             3510..4670
                     /gene="queG"
                     /locus_tag="VN178_15560"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWS82434.1"
                     /translation="MRPVQDRAETRRVLEERAREAGFGLLGVTSAEPLREGGERLREW
                     QEAGMAADMGYMHRPVELLTDPKKLQKSARSVVSLGVSYYPGDHPENEGGGRVARYAW
                     GLDYHEVIKGRLFRLREELEEELGIRIRARGFTDAVPLLERSAAQHAGLGFFGRNSCL
                     INDEIGSYFFIADLIVDLDLDPDPPGKGTCGRCTRCMDRCPTGAIKAPGVVDARLCIS
                     YLTIENRGEIPRHLRPKVGDWAFGCDVCQEVCPYNKTKASRSRWPEFSGEAGSGPYLE
                     IEEVLLIRADEEFEGRFGGTPLTRPGRAGLLRNCCVAAGNLKLEKSLPALADALREDP
                     SPLVRGHAAWALGEIGGAEESLIEASETEKDPWCLQEILLALAGGTTNATLG"
     gene            4683..5498
                     /locus_tag="VN178_15565"
     CDS             4683..5498
                     /locus_tag="VN178_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWS82435.1"
                     /translation="MADQINYYEVLGIPREASQAEIRNAYRNLAKERHPDHPGGSAEE
                     FSRLQEANAVLSDPNRRRQHDEALDLAHAADQLAGLNLDFGSVEDELSAKRRERESRG
                     PSLGERLRGRFGRKEEDPYESETSDPGRGGGRRTRGRYDVSEARWYEPHRFDPEPITL
                     KSGAVSFVAAFLAFIAIGQIGLWAGGVDPGIFGSVTVLDPFMPILYSLVGLVVAYFTF
                     RAAGYAALALAFVAALVVGGQGPDGLLQFTTVGIIVLLVVVYLGNRRESAARR"
     gene            complement(5510..5698)
                     /locus_tag="VN178_15570"
     CDS             complement(5510..5698)
                     /locus_tag="VN178_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82436.1"
                     /translation="MIPRGGYTGGMKMTEDEALREYIRRLDDLMSFVGREELPYSLRT
                     LHMDRLLTMAREARERGA"
     gene            5733..7244
                     /locus_tag="VN178_15575"
     CDS             5733..7244
                     /locus_tag="VN178_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82437.1"
                     /translation="MPTEFRSVEDAVRGIEKSAYEIQTESRLVYSGLKREQNLAEILE
                     KYAWLYNPETVRRVEETYRAETDPENKERLRRVYYYLLEGYIGQRTAPQEDELVSFEA
                     NATVEVDGERIPYHNVPVLIAGEHDFERRTRLRDASLEVVETTNRERLKILRTSLATL
                     ANEFGHSDYTAYIAEKKRIDYSLLRSRMESFLSQTEEIYAALMGRWVEETTSRRLGEI
                     GSHHVSYISRLPQYDEYFRRDRLLGAYERTLSGLGLDPASQKNIHIDAEERATKNPRA
                     RCYAPDPPREVHLLIKPVGGLEDYMAFFHEAGHAQHFGNEDPALDYVERAVSTSYALT
                     EVYSFLLEHLTQNRAWLTGVVGLPEETAGEVAYYAGLTELFFVRRYAAKLAYELDFFE
                     DPLAEGRNRRLYSTTLSAATHFVYPSQNYLNDMDPGYYSADYLRAWITEAMLRRTLEE
                     TYGEDWFARPEAGEFLRGLWATGEKQENEDVARAIGRKPFDTSYLIEQFRGLE"
     gene            complement(7303..8247)
                     /locus_tag="VN178_15580"
     CDS             complement(7303..8247)
                     /locus_tag="VN178_15580"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HWS82438.1"
                     /translation="MRRTSALLLPSLLAATLCAGCLNEGPEVRSVEASSASGGPAETS
                     KENVSGMGGMPEATTIRASRIEGDLAGATDASTGVPFVAKPQAGGGFGYDADTVLAVR
                     YEVNEDYERAVIDLGTGLEPAGHVPEWSLVSQEGNGLLRVTLPSASATCVSDGRFGGG
                     LLQSFHVVRAPEGGMFMDIFTRKAFLYRVVELGDPARLVVDFEPKGGLKAPLPAVSGD
                     TVLVEPRPYTSISDPLTVNGYSRNPEGANAITLIDSEGEILVRRSVPNNDGGRHTWGY
                     FEATLDLPALSGSGTLKVGTENAQDGSSAGVEIPIRTR"
     gene            8415..9377
                     /locus_tag="VN178_15585"
     CDS             8415..9377
                     /locus_tag="VN178_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HWS82439.1"
                     /translation="MIGSRDIEAARRRLQGVALRTPLVGCPRSEEERALYFKPECLQP
                     TGAFKLRGAYNKISSLSPEDRRRGVVAHSSGNHAQAVAYAALALGVKAAIVMPRGASR
                     VKLDATEELGAEVVLVGPGSAERSRRAQELAVEHGYVPVPPYDDEVLMAGQGTIGAEI
                     LEDLPDVETVLVPVSGGGLIGGISAAIKLARPEVRVIGVEPELASDARASLKSGRLVE
                     FPADQVARTVADGLRVQKLGEAPFGHIRAFVDDIIAVTEWEILEAMRRLALRVRLVAE
                     PSGAVTFAAYLFHREELPPSRRTVAVISGGNVEPGLLVEVLSKG"
     gene            complement(9452..10201)
                     /locus_tag="VN178_15590"
     CDS             complement(9452..10201)
                     /locus_tag="VN178_15590"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HWS82440.1"
                     /translation="MAPSTVTLSGLYVYPIKSCAGIPLQSADLSATGLRHDRRWMLVD
                     ETGEFMSQRAHPRMALISIRFSAEHLIVSAPDMEELEIPLYPQAESSIDVRVWGDTNR
                     GALVGEEADGWFGEFLRFPCRLVHKPADDVRLVDSSFAESGDQVGFADGFAFLLISEA
                     SLEDLNMRLEDPLPMNRFRPNFVVRGCGPYAEDGWSRLRIGSVPFRVAEPCPRCAITT
                     VDQETGTRGKEPLRTLATYRRSEGEVFSAAT"
     gene            complement(10252..12012)
                     /locus_tag="VN178_15595"
     CDS             complement(10252..12012)
                     /locus_tag="VN178_15595"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWS82441.1"
                     /translation="MRMSTMFGRTLREAPSDVEVASHRLLLRAGFIRQLAAGIFSYLP
                     LARRSIGKIEKILREEMDAAGGQEVSMPVVQPADVWERSGRYTATGQELVRLGDRRNR
                     EMVLAMTHEEIVAGLAASEVDSYQRLPRLVYQIQTKFRDDPRPRAGLIRAREFTMKDA
                     YSLDRDERGLDEQYRTLYKTYFRIFARCGLPAIAVGADVGIMGGSMAHEYMYLTPIGE
                     DTILICDNCGYTANRQVARFRKSIPEAEEQRPVEKVTTPGADSIETLARSLKIPKSGT
                     AKALFQVATVGGSERFVLAVVRGDMELNETKLANAVGASGLRPALPEEIRAVGAEPGY
                     GSPLGVVGAIVVVDDTIPASPNLVAGANEVGFHFLNVNYGRDFEADTVADIASAGDGS
                     LCPECGYAMRAVRGVEVGNIFKLGTRYSEAFGASFLDNDGRRKPVVMGSYGIGLGRLL
                     ACIAEEHHDENGLVWPVWVAPYHVHIVAAEADERAEGLYERLLSAGVEVLYDDRRESL
                     GAKFKDADLIGAPVRLTLTPRSLQRGGVEIKARSDSAGCVVSIEEVVTAVSKEISRQQ
                     AQIPRGDPVASAPDESTSWA"
     gene            complement(12197..13189)
                     /locus_tag="VN178_15600"
     CDS             complement(12197..13189)
                     /locus_tag="VN178_15600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWS82442.1"
                     /translation="MNKERSRQKMLAFTAREDNEVEMSAAQNRLLRAVIETDSGVRIR
                     QMDDADLERILELRSIVRWSADPRAFDLLRGVRDARWAVAEAPDGDLAGMVGAVPLGE
                     IGVLCHLAVHDGYRGLGLGALLSSWAVAYLRSRGAKMTRLYSTVRAEALYRSLGFQEV
                     TPRTVYRLEDGSRRSRSTERVDGHEVETLTFGDLPELYGVDHWSYGADRSALIFATLR
                     LHPGRGLIARDSTGRIKGYLIRSARGGATRVGPFLASTPEVARLLLDRALSATGDWPV
                     QVTVPGPAECQAHSILQEFGFQGTKDRMRMELGERRATCRAGLVQYGTTPYLAT"
     gene            complement(13407..14372)
                     /locus_tag="VN178_15605"
     CDS             complement(13407..14372)
                     /locus_tag="VN178_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HWS82443.1"
                     /translation="MEYRRLGGSGVKVSEISLGSWLTYGGSVAEEQATACINRAYEIG
                     INFFDTANVYMRGAAEEIVGRALRDFERDSYFLATKVYFPMGEGPNDRGLSRKHITEQ
                     CHASLRRLGVDYVDLYQCHRYDENTPLEETLRALDDLVRQGKVLYVGVSEWTPEQISD
                     ALIIAKEMNLDRIVSNQPQYNMIQRRIEGGVIPLSEREGVGQVVFSPLAQGVLTGKYR
                     PGDAPEEGTRAADPESSRFMQQLMNEEVLSAVDGLRSVASEAGLSMPQLALAWVLRQE
                     NVSSAIIGASRPEQVDDNAAASGVELPADVVFEIDGILEGMIQRG"
     gene            14577..15218
                     /locus_tag="VN178_15610"
     CDS             14577..15218
                     /locus_tag="VN178_15610"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWS82444.1"
                     /translation="MRATLQPGLTNRLTKYLRTTQQLIEEGRDAVSSKELGDFTGINP
                     VQVRRDLNAIGFSGTRGVGYQAYDLVEAVRNILGLRQTYNIALVGAGNLGSAIAASTI
                     LPKRGFVIHDVFDDNPEKIGRTVGNVVVKHIDELKKSVTEAEEIIGIIATPASSAQEV
                     ANLMIDADIRVILNYTDVLLHVPSHVDVHRIDPTAQLMHTLYYLTQAEGQEAS"
     gene            15834..17357
                     /locus_tag="VN178_15615"
     CDS             15834..17357
                     /locus_tag="VN178_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HWS82445.1"
                     /translation="MPGPTHSPGEESAEGGGQLIDFPRGTGSKPPPEYNNLPLQLTSF
                     IGREREIAGLEKLLVGEARLVTLTGSGGSGKTRLALSVAAELAGDYEDGVWWVELASL
                     SDPDLVPQAVASALGMNEAPGRSLIEALVEYLEGREVLLLLDNCEHLVEACAALCDVL
                     LHACPKLRILATSREALGVAGERNWPVPSLSFPDPHSLPRIEELGRIESVRLFVERVR
                     SRRSEFALDSRNAPSVAEVCRALDGIPLAIELAAARIGTLSVAQISERLEHSLGLLTG
                     RDRTSPERQRTLRGALDWSYELLEEEERELFGRLSVFAGGFTLEAAEAVCAGEGIEHD
                     EVLDLLGHVIDKSLVVAEPAEEGALRYRMLEPVRQYGWEKLQESGKAERTRKWHAEYY
                     LALAEEAEPELSGVNEGVWLERLEAEHGNLRAALGWSLEDGDAGLGLRLAGFWYKHGH
                     LSEGRMWLDRELAEGSAAPAIERATALDQAGWIALYQGDLEPSVALLDESLSLFKEL"
     gene            17403..18281
                     /locus_tag="VN178_15620"
     CDS             17403..18281
                     /locus_tag="VN178_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWS82446.1"
                     /translation="MLHQDDDEYLAALCEEAETLRRAFVDRRAIGELIVFLGMVALYE
                     GDLERAITLLDESLNLFRGLASESHVAPDNQGEGIQLSTAIDLVAGQAQEYLWLAVME
                     QGDHRRAVELLEEELRLSLELGNKPKISYCLLGLAAVAALQGRPARAVRLWVAAETLR
                     EEIGLGLPLWDHTPTDYQAILAATRSQLDAATWETARTEGRAMSLERAVEFALRSQEM
                     PEEAGTPPAHPAGLSAREIDVLKLVARGMTNAQVARELFISPNTVNRHLSSIYRKIGV
                     SSRAAATRFASEHNLL"
     gene            18663..18908
                     /locus_tag="VN178_15625"
     CDS             18663..18908
                     /locus_tag="VN178_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82447.1"
                     /translation="MRQRMVKVSVVVRSGTVRFLVAVQARSIRKALGLVRGMYPVARS
                     VFPIEPAGIFVEDPAALAGIVGTEQSRQDAASPSVSE"
     gene            19007..20371
                     /locus_tag="VN178_15630"
     CDS             19007..20371
                     /locus_tag="VN178_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWS82448.1"
                     /translation="MMTSTKMKTANHHFRVLVALALVATMAAMLLATGRSAQAASTTF
                     TVDSTVDAADAIQTDNICDADVSNAQVCTLRAAIEQANTNNNPTEADRINFNIPGTGV
                     KTISPGSALPTITEPVVIDGYSQPGSSPNTLAKGTNAVLNIQLNGTNAGEFSAGLDID
                     APNSVVKGLVINRFGSFGVRALDGGSGSTIEGSFIGTNPSGTLDRGNHTGGVSLFFES
                     ANIAVGDNTPAARNLISGNDGRGVEIFVSTGNEVMGNLIGTKRDGVGSLGNLDEGVSL
                     FGSSDNTIGDGTTEGANTIAFNGLDGVRVRTSTSDEGTDNSVFRNSIFSNGDLGIDLN
                     DDGPTANDAGDPDTGPNNLQNKPLLSSAANASGKSTIKGKLNSNASQTYTVEFYSNPA
                     GTDEGKKFIGQKSVTTDSSGSVSFTFSPATKVPAGQAITATATNGSTGDTSEFSAPRK
                     VVAS"
     gene            20442..20825
                     /locus_tag="VN178_15635"
     CDS             20442..20825
                     /locus_tag="VN178_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82449.1"
                     /translation="MKAPASEYVTLVEVLELTVPYEEFAQAVGAATRRLEAEGVRELV
                     TVHFYGNPGSTEVGAILTFSNRERMIEHIGMISEWEEFRRFFACVRPLDVRVYGKLSE
                     EAEPWIGRFDVLSKKFEEHIAGFVR"
     gene            complement(20916..21293)
                     /gene="gcvH"
                     /locus_tag="VN178_15640"
     CDS             complement(20916..21293)
                     /gene="gcvH"
                     /locus_tag="VN178_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWS82450.1"
                     /translation="MNIPEDLQYTKSHEWVRIEGDTATIGITDHAQDELGDVVFVELP
                     DEGATFDAGESFGTVESVKAVSDLYTPVGGEVVEVNSSLEDAPENINEDPYGEGWIVK
                     LRTSDEPNLLSPEEYEAVVEEES"
     gene            complement(21304..22389)
                     /gene="gcvT"
                     /locus_tag="VN178_15645"
     CDS             complement(21304..22389)
                     /gene="gcvT"
                     /locus_tag="VN178_15645"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWS82451.1"
                     /translation="MAKDDLRHTPLYEEHKNLGARLVDFAGYEMPVQYSGIQAEHRAV
                     RNHAGLFDVSHMGEAIFRGPDAESAVQRLVTRDVARLDVGQAGYSAVCYEDGGTVDDV
                     IVYRREDDFLVVVNASNREKDLEHFRENTGDLDVEVADESDDWALLALQGPEAADLLQ
                     DLTETDLSSLKPFRFVQEEVDGARSIISRTGYTGEDGFEIYLSPDDAPRVWRRLIEAG
                     ATPAGLGARDTLRLEAGMCLYGNELDAETTPLEAGISFAVHLDKEERFIGQRALLLED
                     TEGLRKKLVGFEMEGRGIARHGHHVTSGAEIVGEVTSGTKSPTLEKAIGLALVDPGVD
                     DWFEIVIRDNPVPAKAVPLPFYKRDRG"
     gene            complement(22392..23807)
                     /gene="gcvPB"
                     /locus_tag="VN178_15650"
     CDS             complement(22392..23807)
                     /gene="gcvPB"
                     /locus_tag="VN178_15650"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWS82452.1"
                     /translation="MPFNLIRDKGREGRRGYTLPKQDVEMKGLETILPESALRKEKPH
                     LAEVDEPTVIRHYTNLARENEGIDTTFYPLGSCTMKHNPRANEAAAVVPGFAGLHPLQ
                     PEAQVQGALRVMHELQGFLAEVSGLPYVSLQPLAGAQGELTSILMVKKFFEDAGDDGR
                     TTVLVPSTAHGTNPATASMAGFGAEEVGLDEHGCVDVDELREMVDGSTAALMITNPNT
                     CGVYERNIKEIAEVLHEAGAFLYMDGANMNANMGRMRPAEADVDIMHFNLHKTFSTPH
                     GGGGPGCGAIACTDNLTPFLPEPTVEKEGDAYRFVRPEKSIGRVAGFHGNFGQMLRAW
                     NYIRMHGPELRDVTDMAVLNANYVRAQLRGTYRIPYDELCKHEVVVQPPQGKRALDIA
                     KGLIDYGYHPPTIYFPLVVKEAMLIEPTETESKETLDDFIGTMLELAETSQEDLEEAP
                     THAPTRRLDETRAARQLKARW"
BASE COUNT         4509 a         7451 c         7514 g         4435 t
ORIGIN      
        1 attccgggca gaagacatag aggttgtttc cagggccgga cgcttccgta tagcgttccc
       61 aatctacttt gcagcgccgc agagcgtcca ggtccctaag cgggactacg atggccggaa
      121 gtccggtgga tacttcttgc acgggataat ggtcgttgag gtctgccgct tcgagattca
      181 gagctcgggc gacggaagca tggtccagga ccgcaccgaa ggtgggtggg agctggcgca
      241 tccagagaac ctcgccgaag gtcacgggga tctcaccggc cttcaggttc aacgtgatac
      301 ccccgacggg gccggcgagt agttcgtgtt ggatcacgta cgccgtaccg agggtgggat
      361 ggccggcgaa gggtacctcc tcgcccggcg tgaagatgcg gacgtcgtat cctccctctg
      421 actcttcgtc cgacaagacg aaggtggtct cggaatagtt catctccagg gctatcttct
      481 ggagcgtctc atcgggcagg tcggccccgc cgtgaacgac ggcgagctgg ttgccagcgt
      541 atctctcttc ggcgaagacg tccacgatgt agaaagtgag gctcactgtc cctcctaacc
      601 gaagtcgggg tcgctgcggg agagccgcat ggcgccgatg aacatcttga aggccgtttc
      661 gtagttcacg agacgcgccg gtcttccacg cgctccatat ccgccgagat gtagatcttc
      721 atggcatcgc ctcctcgaca taagggccag cgtggccacg gagtactgtg gtcttccggg
      781 cctcgatcag taccttggca gcggccctcc ggcgtcttcg aggacgtagt ttgagaacat
      841 acggcgcacc acggcgggaa ggtctcctgc gaggtcctcg tacatgccgg ggtcttctct
      901 cagatcgtag agcctggcct ccgtcccgtc gttgcggcta atcatggcgt actcctcgtc
      961 cctggtaaag acgtggtcgt ggtatccgag cgtgaagtgg gacctcgcct cgggctctcc
     1021 acctcgcagc atcacggaga gatcctggcc atccagcggt tgcggaggtt cgatccccag
     1081 gaacccgagg accgtaggcg ccacgtcgtg ggtggaggcg tagtagtcgc tcgtcttccc
     1141 cgctccctta ccctcaggat ggcggatcag gaacgggatg tccgtcacct cgggccagag
     1201 agaggatggg agcttaccga cgacgccgtg ttccccgtgg gcgatgccgt ggtcggagag
     1261 gagtacgagg agggtgttgt cgaagagatc cagctcttcc atcttgtcca gaaagcggcc
     1321 cagccagcgg tcagtcatcg tcacctcgcc cgagtagagg gcgctcatac gccggatctg
     1381 gcggccgcta aggtagtcgg tggccccgtc gaccggcgcg aaaggctcgg ggccctcgta
     1441 accctcatcg tacatggcta cgtactcagc gggagggtcc cacggcgcgt gaggatcgta
     1501 gcagtccacc gtaagaaaaa atggcctctc agacgcggcc tccaggaact cggaggcgcg
     1561 ggtgaagact tgcggggcga accagtcctc ttccgatctg cggtacgccg tgttggcgaa
     1621 gtaccgttgg aagtacgctc tgctccccgt tcccaagacg gctctgttca acagcgcctg
     1681 ctcgaccttc cccggagggc aggtatagag cggtctgtag ctgtctgtgg tctgcccccg
     1741 gatgaaatcg aatgcctcga agccgcggtg gaaattcatg gaggcgtcgt actgctgcag
     1801 gttatccgtt acgaacagcg tctcgtagcc cgcgcttcgc acagtctcgg cgagggtgat
     1861 ctggtagtta ggtatcggct gccagccctg aagaccgacg tctataccct tgtaacgccg
     1921 ccagtcctcg aaaggccagg tcctgaggcc ggtgtgtatg gccctcctcg cgcagatcgt
     1981 cggcgcggac tcggggtaag ccctgctaaa gagcaggctc tctctagcga gggcgtcgag
     2041 gttgggggtc cggatctcct cgttgccgta ggcgccgacg tgatccttcc tcaggctgtc
     2101 gaggatgatc aggactacgt tggtatccct gtcggcagcc ggggtattgc ctgggagatc
     2161 ggggagcaca cccgcgcgtt cgcacccggc gagcaacgat gccccggcca cacccgcgcc
     2221 ggacatcttc aagaactctc ttcgcgttag agtccgcctc agtctctcct cctgcatacc
     2281 gctcgattgc acagacaatc atagagcaag ttcggtgaga agccttatcc tacccgggat
     2341 ctctgcgggc gaagcctccg tccggacgat gtgatccagg tcaagccggt gtacaatccg
     2401 ggcatggcgg cctttcgtta cggacgggag cacttcgagg aaggggagta cgtacgccgc
     2461 gtggaagcga ccccgggcga cggcctcgac cccgtagttc ggatagtgga agatctggga
     2521 accgtcgaga tcccctgcgg caaagtagac tgccgcagta attgcctgga atacctggtc
     2581 agcgccaccg gcagtcgcaa cgactacacc ctggccgagt gcaagatctc cgcgttggta
     2641 agcatcatat agcactttag catttcagca cgctccagcc tccggctgtc gctcgtcagc
     2701 tagtcagctt tcgcatgggc agctccttgc tgactagctg aaagcgaagc ctagcgaagc
     2761 cgggcgaagc gtgctgacaa ggcgcgaagc gccgtgctga agtgctgaaa cgctattcct
     2821 cccaggaata gcgtttctgt tccggtggcc tttcggcgac gtagagccgt tggagggcgc
     2881 ctccggcaag tggcgcgagg aagagtccgg cgaggcccgc aggcagcagg tcacgggcga
     2941 tggtccaggc tacgagaagt ggcgcccctg gcggatggtt ggcaccttct atgaggcgca
     3001 aggggtcgag gacgccaccg ctcgggagga tacccgtcgc gtagacgatg agggtcagga
     3061 tgccggatat gaaccccgtc agaagcgcga gcgcgaggct aaggacgaaa cggggacccg
     3121 aaccttccga gcgttccgac ctgccgcgcc gcaggagcaa ggcgccgccg aatatggggc
     3181 ctgctatgag tcccgcgagc aaccctacgc ccatgtagac gagggccagc gtggtcaggt
     3241 cgccctcgct gctccgtccc ccttgcagta tcaggagcac gaaaggctcg gggaacagcg
     3301 atttgatgaa catgaagccc acgccgtaga cggccccgat tccgagcagg cgcagtagga
     3361 tcacggtcca cccgccgtgc cgccagtgga acattgttcg cttgtattcg tcatcatgct
     3421 ccagaatcta acctccagcg gccgaaacgt cagcaagagc tttcacgagg cgccgtcctc
     3481 gcgcgccgcc gggtacaatc ggcgtcagca tgcgaccagt acaggatagg gcagagacgc
     3541 ggcgtgttct ggaggagcgg gcgcgagaag ccggcttcgg cctcctcggc gtgacgagcg
     3601 ccgagcctct gcgtgagggt ggggagcgtc tgcgggagtg gcaggaggcg gggatggccg
     3661 ccgacatggg ctacatgcac aggcccgtgg agttactgac ggatccgaag aagctccaga
     3721 agagcgcgag gagcgtggtc tctctcgggg tctcttacta tcctggggat catcccgaga
     3781 acgaaggcgg gggcagggtg gcgcgctacg cgtggggcct cgattatcac gaggtcataa
     3841 aggggaggct cttccggctg agggaggaac tcgaggaaga gcttgggatc aggataagag
     3901 cccgcggttt caccgacgcc gtgccgctgc tggagcgctc cgcggcccag cacgctgggc
     3961 tggggttctt cgggcggaac tcctgtttga taaacgacga gatcgggtcg tacttcttca
     4021 tagcggacct gatcgtggac ctcgacctgg atcccgaccc gccgggtaag ggtacctgcg
     4081 gacgctgtac gcgctgcatg gacagatgcc ccaccggcgc cataaaggcc cccggtgtcg
     4141 ttgacgccag gctctgtatc tcgtacctga cgatcgagaa ccggggtgag atcccccgcc
     4201 acctccgtcc taaagtgggt gactgggcct ttgggtgcga cgtatgccag gaggtctgtc
     4261 cctacaacaa gacgaaggct tcgaggagcc gctggccgga gttctccggg gaggcagggt
     4321 cgggacctta cctggagata gaagaggttc tgctgataag ggcagacgag gagttcgagg
     4381 gtcgctttgg gggtacccct ctcacgcgtc ccggccgggc tggcctgttg cgcaactgct
     4441 gcgtggccgc ggggaacctg aagctggaga agtcgctccc cgcgctcgca gacgccctgc
     4501 gtgaggaccc ttcgccgctc gtgcgggggc acgcggcgtg ggcgctgggc gagatcgggg
     4561 gcgcagaaga gtcgctgata gaggcgtccg aaacggagaa ggacccgtgg tgcctgcagg
     4621 agatcctgct cgcgctggca ggcgggacca cgaacgctac cttgggttag aatagggaaa
     4681 cgatggcgga ccagatcaac tactacgagg tgctgggcat cccacgcgaa gcctcccagg
     4741 ccgagatccg gaacgcttac cgcaacctgg cgaaggagcg tcaccccgac catccaggcg
     4801 ggagcgcgga ggaattctcc cggctgcagg aggccaacgc ggtcctctcc gacccgaacc
     4861 gccgcaggca gcacgatgag gcgctcgacc tggcccacgc ggctgaccag ctcgccggcc
     4921 tcaacctgga cttcggctcc gttgaggacg agctctccgc caagcgcagg gagcgcgagt
     4981 ctcgcggacc ttccttggga gagcgcctga ggggccgctt cggcaggaaa gaagaagacc
     5041 cttacgagtc cgagacgagc gaccccggac gcggaggtgg acgcaggacc aggggtcgct
     5101 acgacgttag tgaggcccgc tggtacgaac cccaccgctt cgatcccgag cccataaccc
     5161 tcaagagcgg tgccgtaagc ttcgtcgcag ccttcctcgc ctttatcgct atcggccaga
     5221 tcgggctgtg ggcaggagga gttgatcccg ggatcttcgg gtccgttacg gtcctggacc
     5281 cgtttatgcc catcctgtac tcgcttgtcg ggctggtcgt agcttacttc accttcaggg
     5341 ccgcgggtta tgcggcgctg gccctggcct tcgttgccgc cctcgtcgtc ggcggccagg
     5401 gacctgacgg acttttgcag ttcacgaccg ttggtatcat cgttctgctc gtagtggtct
     5461 acctcggcaa ccgccgcgag agcgccgccc ggcgctgatc ccgacccatc taggctcctc
     5521 tctccctggc ttcccgtgcc atcgttagca gacggtccat gtgcaaggtc cgtagggagt
     5581 agggtagctc ttcgcgtccg acgaagctca tcaggtcgtc gagacgtctt atgtactcac
     5641 gtagtgcctc atcctcggtc atcttcatgc caccagtata gccgccgcgg ggtatcatgc
     5701 gactataagg aatcccagaa ccggaggatt gtgtgcccac cgagtttcgc tccgtagaag
     5761 acgccgtcag gggcatcgag aagagcgcct acgagatcca aaccgagagc aggctcgtct
     5821 acagcggctt gaagcgggaa cagaacctcg ccgagatcct cgagaagtac gcctggctct
     5881 acaatccaga aacggtccgg cgtgtcgagg agacgtatcg tgcagagacc gatcccgaga
     5941 acaaagagcg gctacgccga gtctactact acttgctcga gggctatata gggcagcgga
     6001 ccgctcccca ggaagacgag ctcgtctcct tcgaggcgaa cgccaccgta gaggttgacg
     6061 gcgagagaat cccctaccac aacgtgcccg tgctgatcgc cggcgagcac gacttcgaga
     6121 ggcgcaccag gctgcgggac gcctcgctcg aagtggtcga gacgaccaac agggagcgtc
     6181 tgaagatctt gcgcacgagc ctggcgacgc tggcgaatga gttcggccac tccgactata
     6241 ctgcctacat cgcagagaag aagcggatcg actacagtct gttgcgctcg cgcatggaga
     6301 gcttcctctc gcagacggag gagatctatg cggcgctgat gggccgttgg gtggaggaga
     6361 cgacgagccg gaggctgggc gagatcggca gccaccacgt ctcttacatc agccgcttgc
     6421 ctcagtatga cgagtacttt cgcagggaca ggttactcgg cgcttatgaa agaacgctct
     6481 cgggtctggg cctggacccc gcgtcgcaga agaacatcca catcgacgcc gaagagcggg
     6541 cgaccaagaa ccccagggct cgctgctatg cgcccgaccc tccaagagag gttcacctgc
     6601 tgatcaagcc agtgggcggc ctggaagact acatggcctt cttccacgag gcggggcacg
     6661 cccagcactt cggcaatgag gaccccgcgc tcgattacgt cgaacgcgcc gtctccacga
     6721 gctacgccct caccgaggta tacagcttcc tcctcgaaca tctcacccag aaccgggcgt
     6781 ggctcaccgg ggtggtcggc ttgccagaag agactgcggg ggaggtagct tactacgcgg
     6841 ggcttacgga gctcttcttc gtccgccggt acgcagccaa gctcgcctac gagctcgatt
     6901 tcttcgagga cccgctggcg gaagggcgca accggcgact ctacagcacg acactctccg
     6961 ccgcgacgca cttcgtttac ccgtcccaaa actacctgaa cgacatggac cccggctact
     7021 actcagccga ctacctgcga gcctggatca cagaagcgat gctcaggcgc accttggagg
     7081 aaacctatgg tgaggactgg ttcgcccgcc ccgaggcagg cgagtttctg cgcggcctct
     7141 gggctaccgg cgagaagcag gagaacgagg acgtcgcccg tgcgatcggc cgcaagccgt
     7201 tcgacaccag ctacctcatc gagcagttcc gcggtctcga gtagagcaag agccggcacg
     7261 cattctgcac caaagaacac cgtccgtctc ggaacccatt gctcaccggg tccgaatggg
     7321 gatctcgacg cctgcggagg agccgtcctg ggcgttctcg gttccgacct tcaaggttcc
     7381 actccccgac aacgctggca gatccagggt tgcttcgaag tatccccagg tatgacgacc
     7441 tccgtcgttg ttcgggaccg acctacgcac caggatttct ccctctgaat cgatcaaggt
     7501 gatggcattc gcgccttcgg ggttgcgaga gtagccgttt accgtgaggg ggtccgagat
     7561 actggtgtac gggcgtggct cgacgaggac cgtgtccccg ctcaccgcgg ggaggggcgc
     7621 cttcaggcct cccttcggct cgaagtcgac gacgaggcgt gcggggtctc ccagctcgac
     7681 caccctgtag aggaacgctt tgcgcgtgaa gatgtccatg aacatgcctc cctcgggggc
     7741 gcgcacgacg tggaaactct ggagcaaccc tccgccgaat ctcccatccg atacgcacgt
     7801 cgcgctggcc gaaggcaggg ttacgcgcag caggccgttg ccctcctggc tcacgagact
     7861 ccactccggc acatgaccgg cgggctccag cccggtaccg aggtcgatca ccgcccgctc
     7921 gtagtcctcg ttgacctcgt agcgcaccgc gagcaccgtg tcagcatcgt agccgaaacc
     7981 gccgcccgcc tgcggcttcg cgacgaaagg gacccccgtg gaggcgtcag tagccccagc
     8041 gagatcgccc tcgatccgcg aggccctgat ggtagtggcc tcgggcatgc ctcccatccc
     8101 cgacacgttc tctttcgagg tctccgcagg accgcccgac gcggaagaag cctctacgga
     8161 tctgacctcc gggccctcat tcaggcagcc ggcgcagagc gtggccgcca ggagcgaagg
     8221 tagcaagagg gcggaagtcc gcctcacctc tcacccgcgt ccggtcttcg cacgtccaca
     8281 tcacaatgtt cggtcatcgg cgcgggccat tataggtgcg ttcaccttgc gagaggttta
     8341 acgcgatgtt gcgctcctgt tacgatgttg gattatcgcc ggtcctcact ccaggcgaag
     8401 aggcgcacga aaacatgatc ggatcacggg acatagaagc cgcgcgcagg cgtctgcagg
     8461 gagtggcgct ccgcacgccc ctcgtggggt gtccgcgcag cgaggaggag agggcgctgt
     8521 acttcaagcc ggagtgcctc cagcctaccg gcgctttcaa gctgcgcggc gcgtacaaca
     8581 agatctcctc cctctcccct gaggatcgcc gccgcggggt cgtcgcccac tccagcggga
     8641 atcacgccca ggccgtagcc tacgcggccc tggccctcgg ggtgaaggcc gccatcgtca
     8701 tgccgcgggg ggcatctcgt gtaaagctcg acgcgacaga ggaactcggg gccgaggtag
     8761 tcctggtcgg tcccggcagc gcagagcggt ctcgcagggc ccaggagctt gcggtcgagc
     8821 acggttacgt tccggtgcct ccttacgacg atgaggttct catggccggc cagggtacta
     8881 tcggggcgga gatactggag gacctgcccg acgtggagac cgtgctggtg ccggtcagcg
     8941 gcggcgggct tatcggtggg atctcggccg ccatcaagtt ggcccgtccc gaagtgcgcg
     9001 tgatcggggt cgaacccgag ctcgcgtccg acgccagagc cagcctgaag agcggaaggc
     9061 tggtcgagtt tccggcggat caggtcgccc gcacggtcgc tgatggtctg cgcgtacaga
     9121 agctcggcga ggcgcccttc gggcacatcc gtgccttcgt cgacgacatc atcgccgtaa
     9181 cggagtggga gatcctggaa gccatgcgcc gcctggcgct gcgggtcagg ctggtcgcag
     9241 agccgagcgg ggccgtgacg ttcgccgctt acctgttcca cagagaagaa ctgcccccgt
     9301 ctcgccgaac cgtggccgtg atcagcggcg gtaacgtcga gcctggcctc ctcgtcgagg
     9361 tcctttccaa gggatagggc cggcgacgtc gttcagcgcg gcgtcgcctc cacggggtca
     9421 ccgatgcgga cagtaccgag tgtgtcgtgg atcaggttgc ggccgaaaag acctctccct
     9481 cggatctacg gtaggttgcg agcgtacgca atggttcttt gccgcgggta ccggtttcct
     9541 ggtctacggt agtgatagcg cagcgcgggc acggttcggc gacccggaat ggcacgctcc
     9601 ctatcctgag ccggctccag ccgtcttcgg cgtaagggcc gcagcccctg acgacgaagt
     9661 tcggcctgaa acggttcatg ggcagagggt cttccaatct catgttcagg tcttcgagcg
     9721 aggcttcaga gatcaagagg aacgcgaatc cgtcggcgaa gccgacctga tccccgctct
     9781 ccgcaaatga cgagtcaacc agccgaacat cgtcggcagg cttgtggacg agtcggcaag
     9841 gaaagcgcag gaattcaccg aaccaaccgt cggcttcctc gcccaccaac gcacccctgt
     9901 tcgtgtctcc ccagacgcgc acgtctatcg agctttcggc ctgaggatag agtggaatct
     9961 ccagctcctc catgtccggc gcgctgacga tcaggtgttc ggctgagaaa cgaatagata
    10021 tcagggccat ccgtggatgg gcacgttgcg acataaactc acccgtttcg tcgacgagca
    10081 tccatctccg gtcgtggcgg agtcccgtcg cgctcaggtc ggcgctctgc aaggggatcc
    10141 cggcgcagga tttgatcggg tagacgtaga gcccgctcag ggtgactgta ctcggtgcca
    10201 atctcgtgcg gggcgccatg cacaccatca tccaacgtaa cgccggacgc ttcaagccca
    10261 ggatgtgctc tcatcaggcg ctgacgcgac aggatcgcca cgggggatct gagcttgctg
    10321 tctggagatc tccttgctga ccgcggtaac gacctcttcg atagagacta cgcaacctgc
    10381 agaatcgctg cgggctttga tctccactcc tcccctttgc agggaacggg gtgtcagggt
    10441 caggcgtacg ggcgccccga tcaggtcggc gtccttgaac ttggctccca ggctctcgcg
    10501 ccggtcgtcg tagagcacct cgacgccggc ggagagcaac ctctcgtaga gaccttcggc
    10561 cctctcatcc gcctcggccg cgacgatgtg tacatgatac ggggcgaccc agacgggcca
    10621 gacgaggccg ttttcgtcgt ggtgctcctc ggcgatgcag gccagtagcc gtccgagccc
    10681 tatgccgtat gaacccatca ccacgggctt tcgccgtccg tcgttgtcca ggaagctcgc
    10741 gccgaaggcc tcgctgtagc gcgtgcccag cttgaagatg ttcccaacct cgacaccccg
    10801 cacagcacgc atggcgtagc cgcactcggg acacaatgag ccgtcccccg ccgaggcaat
    10861 gtcggcgacc gtgtcagcct cgaagtccct gccgtagttg acgttcagga agtggaatcc
    10921 cacctcattg gcgcccgcga ccaggttggg ggaggccggt atcgtatcgt caacgaccac
    10981 gatcgccccc accaccccga gaggagagcc gtacccaggc tcggccccga cggccctgat
    11041 ctcctcgggg agtgcgggcc tcaacccgct cgcccccacc gcgttcgcca gcttggtctc
    11101 gtttaactcc atgtcgcccc tgacgacggc gaggacgaac cgctcggagc ccccgaccgt
    11161 ggcgacctgg aagagcgcct tggcagtacc ggacttcggt atcttaaggg agcgagccag
    11221 cgtctctatg gagtcggcgc cgggtgtggt gaccttctct acgggtctct gctcttcggc
    11281 ttcggggata gatttgcgga atctcgccac ctgacggttt gcggtgtaac cgcagttgtc
    11341 gcagatgagg atggtgtcct cccctatggg agtcaggtac atgtactcgt gggccatgct
    11401 cccgcccatg atgcccacgt ccgccccgac ggctatggcc ggtaggccgc accgggcgaa
    11461 gatcctgaag tatgtcttgt agagcgtgcg gtactgttca tccaggcccc tctcgtccct
    11521 gtccaggcta tatgcgtcct tcatggtgaa ctcgcgggcc cggatcaggc ccgccctcgg
    11581 ccgcggatcg tccctgaact tggtctggat ctgatacacg agccttggca accgctggta
    11641 agagtccacc tcgctcgcgg caagtcctgc gacgatctcc tcatgggtca tcgccaggac
    11701 catctccctg ttcctgcggt cgcctagcct gaccaactcc tgccccgtcg cagtgtaccg
    11761 gccagatctc tcccacacat ctgccggctg taccacaggc atcgacacct cctgcccccc
    11821 agccgcgtcc atctcctccc gcaggatctt ctcgatcttg ccgatagacc gccttgccag
    11881 cggcagatac gagaagatcc ccgccgccag ttgccggatg aacccggccc tgagtagcag
    11941 cctgtggctg gcaacctcca cgtccgaagg cgcctcgcgt aacgtccgtc cgaacatcgt
    12001 gctcatgcgc atggcgaaca ggctacaggc tacaggtttc aggcttcagg actgccagtg
    12061 gggcgtacct gtccacccgc caaccttcgt ctcttacctg acgatctaac acacccactc
    12121 ctttctgcgg aggatggcct caaccagaaa gagctcctcc acaacaacct gatgcctgtt
    12181 gcctgaaacc tgatgcctaa gtggccagat acggcgtcgt accatattgg actagtcccg
    12241 cacggcaggt ggccctcctc tcgcccagtt ccatccgcat ccggtccttt gtgccctgga
    12301 agccgaactc ctgcaagatg gagtgcgcct ggcattcggc cgggcctggg accgtcacct
    12361 gtacgggcca atctccggtc gccgagagag cgcggtcgag caggaggcgg gccacctcgg
    12421 gcgtggaagc caggaaagga ccgacgcggg tcgcgccgcc cctcgcgctc cggatcaggt
    12481 agcctttgat ccggcccgta gagtcgcggg cgatgaggcc acgtcccgga tgtagtctaa
    12541 gggtggcgaa gatcagggcc gaacggtcgg ccccgtagct ccagtggtcc acgccgtaga
    12601 gctcgggcag atccccgaac gtgagggtct cgacttcgtg cccgtctact cgctcggtgg
    12661 agcgtgatct ccgggatccg tcctccagcc ggtagactgt gcgtggggtg acctcctgga
    12721 aaccgagcga ccggtagagg gcttccgccc gcacggtcga gtagagcctg gtcatctttg
    12781 cgccacgact tctgaggtag gctacggccc aggatgagag cagggcgccg aggccgagtc
    12841 ccctgtaccc gtcgtggacc gcgaggtgac agagtacgcc gatctctcca agcggcactg
    12901 ctccgaccat gcccgccaga tcgccgtcag gcgcctcggc caccgcccag cgggcatcgc
    12961 gcacgccgcg caggaggtcg aaagccctgg ggtcggccga ccagcgtacg atgcttcgta
    13021 gctccaggat gcgctccagg tcagcgtcgt ccatctgcct gatgcggaca cccgagtcag
    13081 tctcgatcac agcccgtaac aatcggttct gggctgctga catttctact tcgttgtctt
    13141 ctcttgcggt gaaggccagc attttttgcc tcgatctctc tttattcatg gatagagact
    13201 cttccgcgca aaggtttcgg agaaacccag agtgtcgcgg atgccccgag cgcgccctag
    13261 agggacggtc ggggcaggga cggtcgaggg cgccgtcctg taaaggagtt cgaggtctgc
    13321 cttatgtgat accgttccac acccgcaatg ctacaacatg gcaggccgac atggaaggac
    13381 tctgagagaa ggctggccga acggccctag ccgcgctgta tcatcccttc cagtatcccg
    13441 tctatctcaa agaccacgtc cgcggggagc tcgacccctg aggctgcggc gttgtcgtcg
    13501 acctgctcgg gtctcgaggc gccgatgatc gcgctgctca cgttctcctg gcgcaatacc
    13561 cacgcgaggg ccagctgcgg catgctgagg ccggcctcgg acgcgacgga gcgcaaccca
    13621 tctacggccg agagaacctc ctcgttcatg agctgttgca taaagcggct ggactccggg
    13681 tcggccgcgc gagtgccctc ttcgggggca tctccaggac ggtacttgcc cgtgagtacg
    13741 ccctgggcta gtggagaaaa gacgacctgg ccaaccccct cgcgctcgga caggggtatg
    13801 actccgcctt cgatccggcg ttgaatcatg ttgtactgcg gctggttgga gacgatccga
    13861 tccaggttca tctccttcgc aattatcaaa gcgtcggaga tctgctccgg cgtccactcg
    13921 gagacgccga cgtacaggac cttcccctgc cgcacgagat cgtcgagcgc cctcagcgtc
    13981 tcctccagcg gcgtgttctc atcgtagcgg tggcactggt agaggtcgac gtagtcgacc
    14041 ccaagcctcc tgagcgacgc gtggcactgc tcggtaatgt gctttcgcga gaggcccctg
    14101 tcgttcggtc cttctcccat aggaaaatac accttggtcg ccagaaagta cgagtcgcgc
    14161 tcgaagtctc ttagcgcccg gccaacgatc tcctcggcag ccccacgcat gtacacgttg
    14221 gccgtgtcga aaaagttgat gccgatctcg tacgcccggt ttatgcaggc cgtcgcctgt
    14281 tcctcagcga cactcccccc gtacgtcaac cagctcccca gggagatctc gctgaccttg
    14341 accccactac ctccaagccg cctgtattcc accagaacct ccaacgtata gagaacgaga
    14401 tgctaatggt acacacccgg cagcgcgtaa tcttcgtact ctaaggtcca tagcgctgta
    14461 accggccttg cttactgatt tgttgtcggt taagatcatc tttattgcaa gagcggccta
    14521 ttttgttaca atgcccccat cgatactcga tccacgaaga aggagcggaa gcaacggtgc
    14581 gagcaacgct acagcccggc ctgacgaaca ggctgacgaa gtatctcagg accacgcagc
    14641 agctcatcga ggagggccgc gacgcggtct ccagcaagga actaggcgac ttcaccggca
    14701 taaaccctgt gcaggtacgc agggatctta acgccatagg cttctctggc acccgcgggg
    14761 tcggctacca ggcctacgac ctcgtcgagg ccgtgaggaa catcctgggg ctcaggcaga
    14821 cctacaacat cgcgctggtc ggcgcgggca acctcggcag cgccatagcg gcgagcacca
    14881 tcctccccaa gaggggcttc gtcatccacg acgtcttcga cgacaacccg gagaagatcg
    14941 gccgcaccgt gggcaacgtc gtcgtcaagc acatagacga gctcaagaag agcgtcaccg
    15001 aggccgagga gatcatcggc atcatcgcga ccccagcctc ttcggcccag gaggtcgcca
    15061 accttatgat cgatgccgac atcagggtga tcctcaacta cactgacgtc ctcctccacg
    15121 tcccatccca cgtggacgtc caccgtatag accccaccgc ccaactgatg cacacacttt
    15181 attatctgac ccaggcggaa gggcaggaag cctcgtaaag gacttcagca tgtcagcaag
    15241 ccgcttgcag cggcttgtca gcatttcagc ttgtcggcta gtggtcttcg gcactcagcc
    15301 ggggaccact tcttcctgtg gagaactgta cgcattcgcc gagcagaagc gccattcgtg
    15361 cccttgctga agtgctgacg agcggaggcg aagccggagc gtgctgagag gcgcgaagcg
    15421 ccgtgctgac tagctctgaa cagaggaaga gcccggaacg tacttagggg tgataggtgg
    15481 ggaaagggaa aggaggagta acgccccgga ctctttaatt gtgccggttt agtttcgcac
    15541 caaccgacta acaagatgat acatcgctcg tcacactttg taaagtcctt ggaacagaga
    15601 atttagaaac tttttgtaga tatctgccaa atgcacataa agatgtagtg taagtctacg
    15661 gcaagccgcg caagggagtg tggatctggc gattgggtgt cgcttacggc gcgtcagaat
    15721 cttcttgctg cggctggccg acgagagatc cgagggtccg gtctatcggg ggtatcgcga
    15781 ggcgtataca atgccgtaag atcggtaagg aactttggga gcaagtctgc gacatgcccg
    15841 ggccaacgca ttcacctggt gaggaatctg cagaaggtgg cggccaactc atagattttc
    15901 cgcgcgggac tgggtccaag cctccccccg agtacaacaa tctgcccctg cagcttacga
    15961 gctttatagg gcgcgagcgt gagatcgccg gtctggagaa gctcttggta ggagaggcac
    16021 ggttggtaac gctaaccggt tctggaggaa gtggtaagac acgattggcg ttgtcggtcg
    16081 cagccgagct tgcaggagat tatgaggacg gggtgtggtg ggtggagcta gcctcgctct
    16141 cggaccccga cctggtgccg caggccgtag ccagcgctct ggggatgaat gaggcaccgg
    16201 gacgttcttt gatcgaggct ctagtggagt atctggaggg ccgggaagtc ctcctactgt
    16261 tggacaactg cgaacatctg gtggaagctt gcgccgcact ctgcgatgtc ttgctgcacg
    16321 cctgtccgaa gctgaggatc ctggctacca gccgcgaggc gctgggggtg gcgggtgaaa
    16381 ggaactggcc tgtaccatcg ctctcgtttc cggatcctca cagcctgcct cggatcgaag
    16441 agcttggacg aatcgagtcg gtacgcttgt tcgtggagag agtccgctcc cgaaggtcgg
    16501 agttcgcgct cgactccagg aacgctcctt cggttgcgga ggtctgtcgg gcgctggacg
    16561 gcatcccgct cgctatagag cttgcagcag ccaggatcgg aaccctatcg gtggcccaga
    16621 tctcggagag gctcgaacat tcgctcgggt tgctcaccgg cagggatagg acttctccgg
    16681 agagacagcg gaccctgagg ggcgcgcttg actggagcta cgagctgctc gaagaggagg
    16741 agcgggagct gttcgggcgc ctctcggtat tcgctggcgg cttcaccctc gaggctgcgg
    16801 aggcggtgtg tgctggggag ggaatcgaac atgacgaggt gttggacttg ctcggacatg
    16861 tgattgacaa gtctctggta gtggccgagc cggcagagga gggcgcgctt cgctaccgga
    16921 tgctagagcc cgttaggcag tacgggtggg agaagctgca agagtccggt aaggcggaga
    16981 ggacccgaaa gtggcacgca gagtattacc tggcgttggc cgaggaggct gagccggagt
    17041 tgagcggggt aaacgagggg gtgtggctgg agcggttgga ggccgaacac ggcaacctca
    17101 gggccgcact aggttggtcg ctcgaggacg gggatgctgg tctaggtctg cggctggcag
    17161 gattctggta caaacacgga cacctgagcg agggacgcat gtggctcgac cgagaactcg
    17221 ccgagggtag tgcggcgccg gcgatcgagc gggcaacggc actcgaccag gccggttgga
    17281 tcgcactcta ccagggggat ctcgaacctt cggtggctct gcttgacgag agcttgagtc
    17341 tgttcaagga gctatagcac gaaccgggta tcgccgcttc gctcgccaag ctgggacacg
    17401 cggtgttgca ccaggacgac gatgaatacc tggcggcgct gtgtgaagaa gccgaaactt
    17461 tgcgaagggc gttcgtcgac cggcgggcga tcggcgagct gatcgtcttc ttgggaatgg
    17521 tcgcgttgta cgagggcgat ctcgaacggg cgatcacgct gctggatgag agccttaacc
    17581 tgtttcgggg actggcgagc gagtcgcacg ttgcgccgga caaccagggg gaaggtatcc
    17641 aactctccac ggcgatcgac ctggtcgcgg gacaggccca ggagtatctg tggctcgccg
    17701 tcatggaaca gggagatcac cggcgggcgg tggagttgct cgaggaggaa cttcgcctgt
    17761 cgctcgagct gggaaacaag ccgaagatct cttactgtct tctgggactg gcagcggtgg
    17821 ccgccctaca gggacgaccg gcccgggcgg tgcggctgtg ggtggcggcg gagaccctac
    17881 gggaagagat cggtctcggc ttaccactct gggaccatac acctaccgac tatcaggcaa
    17941 tcctggccgc cacgcgctcg cagctggacg cggcgacctg ggagacggcg cggacagaag
    18001 gacgggcgat gtcactcgag cgggccgtcg aattcgccct tcggagtcaa gagatgcctg
    18061 aagaggccgg aaccccaccg gcccatcccg ccggactcag cgccagggag atagacgtcc
    18121 tcaagctggt agccagggga atgaccaatg cccaggtagc aagagagctc ttcatcagcc
    18181 cgaacaccgt aaacaggcat ctcagctcca tctaccgcaa gatcggtgtg agctctcgtg
    18241 ccgccgctac ccgattcgcc tcggaacaca acctcctctg agactcgtat tccctgcaaa
    18301 gcctagagtg atacagaacc cctggcccac ttcctcagcg tataaactgg tcgagtacga
    18361 ctcctacccc cgctctcgac aataccgtca ttcccgcgaa agcgggaatc caccgcacca
    18421 gacgctccgc tcctccagga gcgatctacc cgatacgcat aagccctctt ctccttcacg
    18481 cttttctcat acatccgcac tcatagcacc cgtcagccca tagatatcgt ctggtagggg
    18541 taaccatgcc cctgatctcc taaatggtta cccctaatgg gtcgttctgg tgatgccccc
    18601 tttcctcgtg ggacctagtg tgtcccttgc aagcacagga cccctagcaa ggaggaagag
    18661 cagtgaggca gcggatggtg aaggtgtcgg ttgtggttag aagcggcacg gtacgctttc
    18721 tggtcgcggt ccaagctcgg agcatccgca aggcgctggg cctggtgaga gggatgtacc
    18781 ccgtggcgag gtcggtgttc cccatagagc ccgcgggcat cttcgtggag gaccctgccg
    18841 ccctggcggg gatagtcggc accgagcaaa gccgccaaga cgcagcctca ccgagtgtct
    18901 cagagtgagg aggcgagccg atggattaag cgagcacgca cgaggaaaga cgcagttcct
    18961 tggtagcgat cagagaccga gaagacgcag aaaaggagag atagagatga tgacgagcac
    19021 aaagatgaag acggccaacc accacttcag ggtactggtg gcgctggcgc tcgtagcgac
    19081 gatggcggcc atgctgctag caaccggtag gtccgcccag gccgccagca ctaccttcac
    19141 cgtcgactcc accgtggatg ccgcagacgc aatccagact gacaatatct gcgacgcaga
    19201 tgtctccaac gcacaggtgt gcaccctcag ggccgcgatc gagcaggcaa acacaaacaa
    19261 caaccctacg gaagccgaca ggatcaactt caacatccca gggaccggcg tcaagaccat
    19321 ctctccgggc tcggcactac cgaccataac cgagcctgtg gtcatagacg gctactccca
    19381 gccaggctct tctccgaaca cacttgccaa gggcaccaac gcggtgctga acatccagtt
    19441 gaatgggacc aacgcgggtg agtttagtgc cggcttggat atcgacgccc cgaacagcgt
    19501 ggtcaagggg ttggtgatca accgtttcgg cagcttcggc gtccgtgcgt tggatggcgg
    19561 tagcggaagc accatcgagg gtagtttcat aggtaccaac ccctcgggaa ccctggaccg
    19621 gggcaaccac accggtgggg tgagtctgtt cttcgaatcc gccaacattg ccgtcgggga
    19681 caataccccc gcagcccgta acctcatctc cggcaacgac ggaaggggtg tagagatctt
    19741 cgtttcgacg ggtaacgagg tgatgggcaa cctgatcggc accaagagag acggagtcgg
    19801 gagcctgggc aacttagacg aaggcgtgag cttgttcggc tcctccgaca acaccatcgg
    19861 ggacggtacg actgaagggg ccaacaccat tgccttcaac ggcctggacg gagtgcgggt
    19921 tagaacctcg acctcggacg aaggcacgga caacagcgtc ttccgcaact cgatcttctc
    19981 gaacggggac ttgggaatag acctgaatga cgacggcccc accgcgaacg acgccggcga
    20041 tcccgacacc ggtcccaaca acctccagaa caagcctctc ctctcctctg ccgccaacgc
    20101 ctccggtaag agcaccataa agggcaagct caacagcaat gccagccaga cctacaccgt
    20161 cgagttctac tcgaacccgg ccggcacaga cgaggggaag aagttcatcg gccagaagtc
    20221 cgtcaccacc gactcatcgg gcagcgtctc cttcaccttc tctccggcca cgaaggtccc
    20281 cgcggggcag gcgataacag cgacggcgac aaacgggtca acaggagaca cctccgagtt
    20341 ctccgcgcca cgcaaggtgg tggcgtccta gagagatgaa ctagaatcaa caagcggggg
    20401 cggagctctc tgtgctccgc ccctcagaaa aggatacggg catgaaggca ccagcaagcg
    20461 aatatgtaac cctcgtcgag gtgctcgagc tgaccgtacc ttacgaggag ttcgcgcagg
    20521 ccgtaggggc cgccacccgc cgcctcgaag ccgaaggggt tagggaactc gtcacggtcc
    20581 acttctacgg caatccaggc tccaccgagg tgggtgccat cctcaccttc tccaatcgtg
    20641 agcgtatgat cgagcatata gggatgatct cagagtggga agagttccgg aggttcttcg
    20701 cctgcgtgag gcccctggac gtgcgcgttt acggcaagct cagcgaggaa gcggagccgt
    20761 ggatcgggcg gttcgacgtc ctcagcaaga agttcgagga acacatagca ggcttcgtcc
    20821 gctaaccttc acgaacaaga gtgtacgttc tcgccggacg ccatccgcag gttccaggga
    20881 tgcgttctcc tggcttctga gagaaagcgt gtctgctacg actcctcttc gacgaccgcc
    20941 tcgtactctt cgggggagag gaggttgggt tcgtctgagg tgcggagttt gacgatccag
    21001 ccctcaccgt aagggtcctc gttgatgttc tcgggcgcat cctccaggga ggagttgacc
    21061 tcgacgacct caccgccgac cggcgtgtaa agatcagaga cggccttgac gctctccacg
    21121 gtgccgaagg actctccggc gtcgaaggtg gctccttcgt cgggcaactc gacgaagacg
    21181 acgtcgccga gttcgtcctg ggcgtgatcc gtgatcccta tggtcgcggt gtcgccctct
    21241 atgcggaccc actcgtggct cttggtgtac tgaaggtctt caggtatgtt caaggctctc
    21301 cccctatcct ctgtcgcgct tgtagaacgg tagcggcacg gccttcgccg gtaccgggtt
    21361 atcccttatc acgatctcga accaatcatc cacccctggg tctacgagcg caagccctat
    21421 agccttctcc agagtaggag atttggtgcc gctggtgacc tcgccgacga tctcggcccc
    21481 gctcgtcaca tggtggccgt ggcgtgctat cccccgcccc tccatctcga agccgacgag
    21541 cttcttgcgc agcccctcgg tgtcctcgag caagagcgcc ctctgcccga taaagcgctc
    21601 ctccttgtcc aggtgcaccg cgaaggagat gccggcctcc agaggcgtgg tctccgcgtc
    21661 gagctcgttc ccgtagaggc acatcccggc ttcgaggcgc agcgtgtccc tggcgccgag
    21721 tcctgcaggg gtggcgcccg cctctataag gcggcgccag acgcgcgggg catcgtccgg
    21781 ggaaaggtag atctcgaacc cgtcttcgcc cgtgtagccg gtgcgagaga tgatactcct
    21841 ggcgccgtca acctcttcct gcacaaagcg gaatggcttg aggctcgata ggtccgtctc
    21901 cgtgaggtcc tgaagtagat cagccgcctc agggccttgc agcgccagga gcgcccagtc
    21961 gtcggattcg tcagcgactt ccacgtcgag gtccccggtg ttctcgcgga agtgttcgag
    22021 atccttctca cgattggagg cgttgaccac gacgaggaag tcatcctcgc gccggtagac
    22081 gatcacatcg tccaccgtcc ccccgtcttc gtagcagacc gccgagtaac cggcctgccc
    22141 gacgtccagg cgggcaacgt cgcgggtgac gagccgctgg acggcgcttt cggcgtctgg
    22201 gccacggaag atcgcctcgc ccatgtgcga gacgtcgaag agcccggcgt gattgcgaac
    22261 cgccctatgc tcggcctgta tgcccgagta ctgcacgggc atctcgtagc cggcgaagtc
    22321 cacgagtcgg gccccgaggt tcttgtgttc ctcgtagagc ggggtgtgcc tgaggtcgtc
    22381 tttcgccacc cctaccacct ggccttgagt tgcctggcgg cgcgggtctc gtcgaggcgg
    22441 cgcgtggggg cgtgggtcgg cgcctcttcg aggtcttcct ggctcgtctc ggccagctcc
    22501 agcatcgttc cgataaagtc gtctaaggtc tccttggact ccgtctcggt gggttcgatc
    22561 agcatcgcct ccttgacgac tagtgggaag tagatcgtgg gcgggtggta cccatagtct
    22621 atgagtccct tggcgatatc gagggccctc ttgccctgcg ggggctgcac gaccacctcg
    22681 tgcttgcaca actcgtcgta gggtatgcgg taagtgcccc tgagctgcgc ccgtacgtag
    22741 ttggcgttca ggaccgccat gtccgtcacg tccctgagct cgggcccgtg catcctgatg
    22801 tagttccagg cccttagcat ctgcccgaag ttgccgtgga agcctgccac gcgaccgatg
    22861 ctcttctcgg gacgaacgaa gcggtacgcg tcgccctcct tctctaccgt gggctcgggg
    22921 aggaaggggg ttaggttatc cgtgcaggcg atggcgccgc agcctggtcc gccgccgccg
    22981 tggggggtgg agaaggtctt gtgcaaattg aagtgcatga tgtctacgtc ggcctcggcc
    23041 gggcgcatgc ggcccatgtt cgcgttcatg ttcgcgccgt ccatgtacaa gaaggcgcct
    23101 gcctcatgca aaacttcagc tatctccttt atgttccgct cgtagacgcc acaggtgttg
    23161 ggattggtga tcattagcgc agccgtcgac ccgtccacca tctcccttag ctcatccaca
    23221 tctacgcacc catgctcgtc gagccctacc tcctctgccc cgaaccccgc catagaggcc
    23281 gtcgccgggt tggtcccatg cgccgtcgaa ggtacgagga ccgtggtgcg cccgtcatcc
    23341 cctgcgtcct cgaagaactt cttgaccatc aggatgctgg tcagctcccc ctgcgccccg
    23401 gcgaggggct gcaaggagac gtaaggcaag ccggagacct cggcgaggaa gccctgtagc
    23461 tcgtgcatga cccgtaaggc tccctggacc tgggcttcgg gctggagcgg atgtagtccg
    23521 gcgaagccgg ggactacagc ggctgcttcg ttcgcgcgcg ggttgtgctt catcgtgcag
    23581 gagccgagag ggtagaacgt agtgtcgatt ccctcgttct cgcgcgcgag gttggtatag
    23641 tgccggatca cggtcggctc gtctacttcg gcgaggtgtg gcttctcttt ccgcagcgcc
    23701 gactcaggca ggatagtttc gagccctttc atctccacgt cctgcttcgg gagagtgtag
    23761 cccctgcgcc cctcgcggcc tttgtcccgg atcaggttaa aaggcatcgg caaccacctc
    23821 cacgaaggcg tcgagttcgt ctttggtccg cttctccgtc accgcgacga gcatcgcccc
    23881 gtccccgaga tccagcccgc ctacaatac
//
LOCUS       DATMVF010000298         6578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_85071, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000298 DATMVF010000000
VERSION     DATMVF010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85071"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VN178_15655"
     CDS             <1..261
                     /locus_tag="VN178_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HWS82453.1"
                     /translation="RRPFAAVGDHVLLPFADSISEADAALAPRLTPEVLRSIVGMIPE
                     EWLADEPGFESAEDVRSAYIAYLNTRLQAPRIWVGALEETSR"
     gene            258..641
                     /locus_tag="VN178_15660"
     CDS             258..641
                     /locus_tag="VN178_15660"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HWS82454.1"
                     /translation="MRLFEYALLRVVPRVERGEFINAGVVLYCQDAKFLDARIHLDPE
                     RLRALDPNLDPESVQAHLEVARRVCEGGAEAGAVGMLPQGQRFGWLVAPRSTVVQPSP
                     VHTGFADDPEEAIEHLLRVMVRAPA"
     gene            complement(952..1539)
                     /locus_tag="VN178_15665"
     CDS             complement(952..1539)
                     /locus_tag="VN178_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82455.1"
                     /translation="MLSYPLDLSFKIVTIGTRIRIKDATGRQVAYVRKKKFKLKEDIR
                     VYEDEEQSRLLLRMKADRMIDFSARYAVSGPDGHPIGAVGRRGMKSLWRGAYVLDNAY
                     DMDAGSIREDNPWTKVFDGMMESLPLGDALGGLFFNPAYVAELGGRDVLRIKKQPSVL
                     ESSFRIEKLADFSEDEEDLLLAGIVMMVLLERDRG"
     gene            complement(1514..1819)
                     /locus_tag="VN178_15670"
     CDS             complement(1514..1819)
                     /locus_tag="VN178_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82456.1"
                     /translation="MLDSREISPPVVIPDPVSVTESRDVTELLLGVLRSIEPYEACIL
                     EAEQDGDFEVAAFLRELRRQDLIRAREATRLLRRTFEAAYVSVAKTGGSYVELSVGS"
     gene            complement(1956..2414)
                     /locus_tag="VN178_15675"
     CDS             complement(1956..2414)
                     /locus_tag="VN178_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWS82457.1"
                     /translation="MEAKDHNYGVAGDEVAEEQGPVTESRGPVTMGTQEEKTMSVLAH
                     LSIFLNLVTGFLGPVAALIIWLVYRDRSREVGFQALQSMWYQVAWLVILVAGWTVTTL
                     LMLVLIGFLLVPVMALITVVPFVHSAYAAYRVSKDGGYRYPFIADMIEAR"
     gene            2750..3412
                     /locus_tag="VN178_15680"
     CDS             2750..3412
                     /locus_tag="VN178_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS82458.1"
                     /translation="MGRSRDDHETGPTRGGHPVFAALYDLVGASAERRWMGARRRRLL
                     AGARGAVLEIGGGTGANLAHYRNVDRVTIAEPDPFMRSRIGPKLEDARVPVEVSAAGA
                     EALPFPDGSFDTVVSTLVLCTVPDQAAALDEVRRILRPGGRLLFIEHVRAAGSMARWQ
                     DRLEPLWRRLFGGCHPNRDTVAAIEEAGFEIETFESFYPPDPLSVLTPHVQGSATVRL
                     AA"
     gene            3470..3928
                     /gene="lspA"
                     /locus_tag="VN178_15685"
     CDS             3470..3928
                     /gene="lspA"
                     /locus_tag="VN178_15685"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWS82459.1"
                     /translation="MSRRTGLLFALALALAVFVVDQGIKAIVEGSMRVGESITLITGF
                     LSLTYIKNDGGAFGILGGSQLVLLVGSTVAVVVVLWMLLSGRPSVLTMLGCGLILGGA
                     AGNLLDRLSSGEVTDYVHFSFWYIFNAADAAIVAGVGLLLFSALRTSEKG"
     gene            complement(3925..4320)
                     /locus_tag="VN178_15690"
     CDS             complement(3925..4320)
                     /locus_tag="VN178_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWS82460.1"
                     /translation="MGAVEQVLVAEREWLLAHLHVDVPALERLMDPDYLQIGAGGDAL
                     DKEQVLASFRSGERHWDEAHSDEHRVRVYGNTAVVVGRWRASGVNAGRPFDYQARFVS
                     VWVRRDGEWRMVSDQSTPITSQAVPKMGG"
     gene            complement(4348..6330)
                     /locus_tag="VN178_15695"
     CDS             complement(4348..6330)
                     /locus_tag="VN178_15695"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWS82461.1"
                     /translation="MAKNLVTKILEDHMVEGALDPGEEVGLRIDQTLLQDATGTMACL
                     QFERMGVERVRVDLAVQYVDHNVIQLDFKNPDDHRFLQGFAARYGLHYSRPGNGICHY
                     LHVERFARPGATLVGADSHTTSSGALGSIAIGAGGLDVALVMAGQPFQTPAPKVVGVE
                     LTGTLSDWVTPKDVILELLRRRGVRGGLGRIFEFYGPGVANIEVTGRTTICNMIAELG
                     ATTGVFPSDERTREWLRMQQREEDFVELSADEGADYDEREHIELDELEPLIAQPHSPG
                     NVVPVSEVAGTRAAQVCIGSSVNSGFEDLAVAAAVLRDQTVYPGLVMTVTPGSRQILD
                     SIASTGVYADLVRAGARMLEPVCGPCVGMGQAPPSDSVSVRTFNRNFPGRSGTMTDQV
                     YLASPTTAVATALRGAITDPRELGEYPALPEAPANPAVDDRQILAPAPPQEASGIEIV
                     RGPNIEPPPEAPELPDSLECTVTIVVEDDISTGDLAPDGVEVMSFRSNVPEIARFTFR
                     RFDPEYHGKARESAPGFIVGGHNYGQGSSREHAALAPLHLGIRAVIAKSFARIHRRNL
                     ISQGILPLRLEDESDYDSFEQGQTWELPEIRDRLRNGDEEVPLKREGDEVTLLAEYSQ
                     RERDILLAGGILRQLREKTEDKQPQAPDRVSGEAEH"
BASE COUNT         1132 a         2156 c         2032 g         1258 t
ORIGIN      
        1 cggcgtcctt tcgcggccgt cggcgaccat gtccttctcc ctttcgccga ctccatatcc
       61 gaggccgacg cggcgctcgc cccacggctc accccggagg tactccgctc catcgtaggc
      121 atgatccctg aagagtggct cgctgacgag cctggtttcg agagcgccga agatgtacgt
      181 tccgcctata tcgcctacct gaatacccgt ctgcaggcgc cgaggatctg ggttggagcc
      241 ctggaggaga ccagtcgatg agacttttcg agtacgcgct cctgcgcgtg gtgccacgtg
      301 tcgagcgcgg tgagttcata aacgctgggg tggtgctcta ctgccaggac gcgaagttcc
      361 tcgacgcgcg catccaccta gatccggaac gcctgcgggc gctcgacccc aacctcgacc
      421 ccgagagcgt acaggcgcac ctggaggtcg cacgccgggt gtgcgaaggc ggagccgaag
      481 ccggtgctgt ggggatgctg ccccagggcc agcgcttcgg atggctcgtc gcaccccgca
      541 gcaccgtggt ccaaccttct cccgtccata cgggcttcgc cgatgacccc gaggaggcca
      601 tagagcacct cctgagggtc atggttcgcg caccggcgta gaacaagcac aaacgacgaa
      661 ggttacggca tttcgccttc cacgatgcct cgcaaactcc tatttgcttt cggctacacg
      721 ttagcccgcc gattatgcca gcagcaatgt ttttcgctaa cgaagaactt cctattctgc
      781 gctccttacg aaacgaagca ctgtatcact cgctacaacg gcctcgagga acgtggcatc
      841 gagttcgtgc ctttgcgcga gtggatgggt atcatgacac ccggcaagat caggttggga
      901 atcggcttgg tcagcggacc acgacgaccg cgcaccttgc gtggaccgcc ctcaacctcg
      961 gtccctctcg agcagcacca tcatgacgat gccggcaagc agtaggtcct cctcgtcttc
     1021 ggagaaatcg gcgagcttct cgatgcggaa gctgctctcc aggacggagg gttgcttctt
     1081 gatgcgtagt acgtccctgc cgcccagctc agccacataa gccgggttga agaagaggcc
     1141 accaagagcg tcgccgagag gcaaggactc catcatgccg tcgaagacct tggtccaggg
     1201 attgtcctcg cggatcgaac ccgcgtccat gtcgtaggcg ttgtccagga cgtaggcgcc
     1261 tctccagagt gacttcatcc cccgccgtcc gacggccccg atagggtgtc cgtcgggacc
     1321 gctgaccgcg tacctggcgc tgaagtcgat cattctgtcg gccttcatgc ggagcaggag
     1381 acggctctgt tcctcgtcct cgtagaccct gatgtcctcc ttgagcttga acttcttctt
     1441 acggacgtac gcgacctggc gccccgtggc gtccttgatc cttatgcggg tcccgatcgt
     1501 gacgatcttg aagctaagat ccaacggata gctcaacata cgagcctcca gtctttgcga
     1561 cgcttacgta agcggcttcg aaggttctcc ttagtagccg ggtcgcttcc ctggcccgga
     1621 tgaggtcctg gcgcctcaac tcgcgcagga aggcggcgac ctcgaaatcc ccatcttgtt
     1681 cggcctcgag gatacacgcc tcgtagggct cgatgctcct caatacgccg agcagaagct
     1741 ccgttacgtc gcgactctca gtaaccgaca cgggatcagg gattaccacc ggtggagaaa
     1801 tctccctgct gtccagcatc tctatcctcc tcgaatatcc acgtttctta cgtatacgca
     1861 cgacacgtgc aaaaggtgac agcgccgaat caaagtcccc tcggacgacg cccccaaccc
     1921 ggttggatca ggagcgccgc ttctcaggct gacttctaac gggcttcgat catgtcggcg
     1981 ataaacggat aacggtaccc gccgtccttg ctcacccggt aggccgcgta ggcgctgtgc
     2041 acgaacggga ccactgtgat cagggccatc acggggacga gtaggaagcc gatcaggacc
     2101 agcatcaaga gcgtcgtcac ggtccatccc gcgaccagga tcacaagcca ggccacctgg
     2161 taccacatcg actgcaaagc ctggaaacct acctctctcg accggtctct atagaccagc
     2221 cagatgatca gggcagccac gggcccgagg aaccctgtga ccaggttgag gaagatgctg
     2281 aggtgcgcga gcaccgacat cgtcttctcc tcttgcgtac ccatcgtaac cggaccccgg
     2341 ctctcggtca cagggccctg ctcctcggcg acctcgtcac cggctacccc gtagttgtgg
     2401 tccttcgctt ccattgaggc tcctctcgtc acgctaccat ttacatagga aagttactct
     2461 cgaaccacca gttcgacatc ggtcccggga ccgaatgtgg gtaggtccgc agtccagcat
     2521 ttcagcacgc tcagcccttc gggcttcgct cgtcagcacg gcgcctttga cgccttgtca
     2581 gcacttcagc acggcggctt cgccgccttg ttagccagtc agcaaggacc tattcatacg
     2641 aaagctgaaa tgctgacaag cgacagccgc aggctggagc gtgctgacta gctgaaatgc
     2701 tgaagtggct tcgccacttt cgacgtggca gactctgggt ggagtgccca tggggagatc
     2761 gcgcgacgac cacgaaaccg gcccgacgag aggcggacat cccgtgttcg cggcgctcta
     2821 cgacctggtg ggcgcctctg cggagcggag gtggatgggt gcgcgcagga ggcggttgct
     2881 cgccggggcc cgcggcgccg tgctcgagat cggtggtggc accggcgcta acctggccca
     2941 ctaccgtaat gtagaccggg taaccatcgc cgagcccgac ccattcatgc gcagcaggat
     3001 cggcccgaag ctcgaggacg cccgcgtccc cgtcgaggtc tcggcggccg gggcggaggc
     3061 gctacctttt cccgacggga gcttcgatac cgtggtctcg acgctcgtcc tgtgcacggt
     3121 cccggatcag gcagccgcac tggacgaggt caggcgcatc ctgcgtcctg gcggacggct
     3181 gctgtttatc gagcacgtac gcgctgctgg ttccatggcc cgctggcagg accgcctgga
     3241 gcctctctgg aggaggctct tcggcggctg ccaccccaat cgagacaccg tcgcggcaat
     3301 cgaggaggcc gggttcgaga tagagacgtt cgagagcttc tacccgccgg accccctatc
     3361 tgtcctcacg ccacacgtcc agggttcggc gacggtccgg ctcgccgctt gagaatcatg
     3421 ctaggatcaa ggcgccgatg agtggtcccg agatgaatcc cgcttcgtga tgagccgccg
     3481 gaccggcctg cttttcgccc tcgctctcgc gctcgctgtc ttcgtcgtcg accagggaat
     3541 aaaggccatc gtagagggtt cgatgcgggt cggggagagc atcaccctca ttacgggctt
     3601 cctcagcctc acctacataa agaacgacgg cggggccttc ggcatcctcg gtggaagcca
     3661 gttggtcctc ctcgtcggga gcaccgtcgc cgtcgtggtc gtgctctgga tgctgctgtc
     3721 ggggagaccg tcggtgctca ccatgctcgg gtgcggtctg atcctgggcg gcgccgcggg
     3781 caacctgctc gaccgcctga gcagcggaga ggtcacggac tacgtccact tcagcttctg
     3841 gtacatcttc aacgccgccg atgccgcgat agtagcgggc gtcggactct tgctgttctc
     3901 cgcgctgcgt acctcggaga agggctagcc acccattttc ggcacggcct gcgaggttat
     3961 gggggtcgac tggtcgctga ccattcgcca ctctccgtcg cggcgaaccc acacggaaac
     4021 gaagcgcgcc tggtagtcga aaggtcgtcc ggcgtttact ccacttgccc gccagcgtcc
     4081 gacaacgacg gccgtgttcc cgtagacgcg gacgcggtgc tcgtcgctgt gggcctcatc
     4141 ccagtggcgc tcgccggacc ggaacgaagc caggacctgc tctttgtcta acgcatctcc
     4201 tccagcaccg atctggaggt agtcgggatc catcaatcgt tcgagcgcag gtacatcgac
     4261 gtgcaggtgg gcaaggagcc attcgcgctc cgcaacgagg acctgctcga ccgcgcccac
     4321 cctactactt gctcgcgtcg ctaccgatca gtgctcggct tctcccgaca cacggtccgg
     4381 cgcctggggc tgtttgtcct ccgtcttctc gcgtaactgg cgcagtatgc cgccggcgag
     4441 gaggatgtcg cgctctcgct gcgagtactc ggcgaggagc gttacttcgt cgccctctct
     4501 cttcagtgga acttcttcgt cgccgtttct taggcggtcc cggatctccg gcaattccca
     4561 ggtctgcccc tgttcgaagg agtcgtagtc cgattcatcc tcgaggcgca aagggaggat
     4621 gccctgggag atcaggttcc tgcggtggat gcgggcgaag ctcttcgcga ttacggccct
     4681 gataccgagg tgcaaaggcg ccagggccgc atgctcacgg ctagacccct gcccgtagtt
     4741 gtgcccgccc acgataaagc ccggcgccga ctcccgggcc ttcccgtggt actcggggtc
     4801 gaagcggcgg aaggtaaacc tggcgatctc gggcacgttc gagcggaacg acattacctc
     4861 cacaccgtca ggcgcgaggt cgcccgtcga gatatcgtcc tcgaccacga tggtgaccgt
     4921 gcactccaga gaatccggga gttcgggggc ttccggcggc ggctctatgt tcggaccgcg
     4981 aacgatctcg atccctgatg cctcctgggg cggcgcgggg gccaggatct ggcggtcgtc
     5041 taccgcgggg ttggcgggag cctcggggag agccgggtac tccccgagct cacgggggtc
     5101 cgtgatggcg ccccgcaggg cggtcgcgac cgccgtggtc ggactcgcca ggtatacctg
     5161 gtcggtcatg gtccccgagc ggccggggaa gttacggttg aaggttctga cgctgacaga
     5221 atcggaaggc ggcgcctgtc ccatcccgac gcacggtccg cacacgggct cgagcatgcg
     5281 cgcacccgcc cgcaccaggt cggcgtagac gccggtgctt gcgatagagt cgaggatctg
     5341 gcggctcccc ggcgtcacgg tcatgacgag accggggtag accgtctggt cccgcaagac
     5401 agcggcggcg acggccaggt cctcgaagcc ggagttgacc gagctgccga tgcacacctg
     5461 cgcggccctg gtgccagcca cctcgctcac ggggacgacg ttgccgggcg agtggggttg
     5521 cgctatcagg ggttcgagct cgtcaagctc gatgtgttcg cgctcgtcgt agtcggcgcc
     5581 ctcgtcggcg gagagttcca cgaagtcttc ctcgcgctgc tgcatcctca gccactcccg
     5641 cgtcctctcg tccgagggga agacgcccgt ggtagcaccg agctcggcga tcatgttgca
     5701 gatcgtggtc cgtcccgtaa cttctatgtt cgcgactccg gggccgtaaa actcgaagat
     5761 gcggccgagg ccacccctga cgccgcgacg cctcaagagc tccaggatca catccttcgg
     5821 cgtcacccag tcagagaggg ttcccgtaag ctcgacgccg acgaccttgg gagcgggggt
     5881 ctggaagggc tgcccggcca tcacgagggc cacgtcgagc ccgccggccc cgatcgctat
     5941 ggaccccaga gctccggagg aagtggtatg cgaatccgcc cccacgagcg tcgcgccggg
     6001 tctggcgaag cgctccacgt gcaggtagtg gcagatgccg ttcccggggc gggagtagtg
     6061 gagaccgtat ctggcggcga agccctgcag gaagcggtgg tcgtccgggt tcttgaagtc
     6121 gagttggatg acgttgtggt cgacgtactg gaccgcgaga tccaccctga cgcgctccac
     6181 gcccatcctc tcgaactgca ggcaggccat cgtgcccgtt gcgtcctgga gcagtgtctg
     6241 atctatcctc agccctactt cctcgccggg atccaacgcc ccttcgacca tgtggtcctc
     6301 gaggatcttc gtgaccagat tcttcgccac agtccatcac ccccgccttg cgtttccaag
     6361 ctcgtctact aattctacat acccgtcttt ggcggtttac ccgcggacga gtcgatgaat
     6421 ccgtagcggg ggctctgcgt tggcgcgtcc ccatgacagc ccagaccgcg tgggtccggc
     6481 gtcgcgccgg actcccagta aggacgtccg cgatgccctc cgctattggt ggggcataaa
     6541 agcggaagtt tgtttcagcg acttccgctc caggggct
//
LOCUS       DATMVF010000299         7087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_458711, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000299 DATMVF010000000
VERSION     DATMVF010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458711"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VN178_15700"
     CDS             <1..662
                     /locus_tag="VN178_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWS82462.1"
                     /translation="AGDEEIRCIILLGEGRGFSAGADLAQIMESADGDPDLGEYLRTT
                     YSRLVKRMVGIQKPIVAALHGPVYGAGVGLALACDLRVAAKNAKFSVAFIKIGLMPDA
                     GATFLLPRVVGLGKAMEMSMLGDAVDAEEALRIGLVNKVVADDSLSEEVRNLAGRLAT
                     MPTSALGRMKHALYASFERDLETALEAEAEGQTFCGYTQDHKEGVTAFFEKREARFTG
                     L"
     gene            complement(710..1147)
                     /locus_tag="VN178_15705"
     CDS             complement(710..1147)
                     /locus_tag="VN178_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82463.1"
                     /translation="MSDLERRRGEVSKPGGGNGYLYLTLGVGFVVAAIMVIATAGVPA
                     AGVGFVIPAAIFLGLALKDFQKQRPVLPQPANKERELLSAIKANGGSITPAEAAMETS
                     LTVREADGMLSELAGGGHLAVESRDGALFYSLPGRRRPELEEW"
     gene            1338..2090
                     /locus_tag="VN178_15710"
     CDS             1338..2090
                     /locus_tag="VN178_15710"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HWS82464.1"
                     /translation="MTRQEIVPERLAGATAVVIDVFMATTTLVTILENGARDIYPVAS
                     LEEADEVGAKLDAAGVLRGGEQDAARIEGYDHGPFPEEYAPEVVGGKDVIFVTTNGTR
                     AIADVAPADTVLLGTLRNAGAVANHLEASGTDSIYLVCAGAGGRFNVEDFLGAATVLS
                     RMDVDGYRLNDGALLALDFAERHEEDVYETLKRSRAGRWFVENDRMDAFDFVADMGAS
                     GLVPEVAGGKLRRANEQERYVGDAERSLGGWT"
     gene            2087..3145
                     /locus_tag="VN178_15715"
     CDS             2087..3145
                     /locus_tag="VN178_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWS82465.1"
                     /translation="MSETIEIREGEDFDLEAIERYLRAHVEDVPEGELEVSQFPSGAS
                     NLTYLLRVGDWEGVLRRPPLGPVPPKAHDMGRESGILARLNAVYPLAPKPYFFCEDES
                     VIGAPFYVMERRKGIVIDDTFPDGVDNDEELCQGISRTLLDTLVELHAVDVGKAGLGD
                     LGKPEGFLRRQTEGWISRYEEAKTDEIEEVGPLTNWLARDIPESPSPAVIHNDYKLNN
                     LVLDPEDLTSVRAVLDWEMATVGDPLFDLAVSLSYWIEPGDPDELAAVLPTVTVTPGF
                     MTRRELIDRYERESGRDLSEMHWYVVFGYFKLAGILQQIYARYKNGQTTDERFATFGE
                     RVRTLILHAENLSRTGEV"
     gene            3416..4177
                     /gene="fabG"
                     /locus_tag="VN178_15720"
     CDS             3416..4177
                     /gene="fabG"
                     /locus_tag="VN178_15720"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWS82466.1"
                     /translation="MGRLDDRVAMVTGASRGIGEATARRFAREGARVCLTDVKLEGAL
                     STARQLVNEGMDAFAARVDVTSRTEVENAVKEIVDRYGRLDILVNNAGVTRDNLFYKM
                     TDEDWRTVLNVHLGGAFLCTRAAQRHMVERNYGRIVNVSSTSALGNSGQTNYSAAKAG
                     IQGFTKTLAIELGRYGVTVNAVAPGFIETEMTRETAARLGVDFDDFVAERVQSIPAGR
                     AGWPEDVAAAILYLASEEAAFVSGQVLYVAGGPTD"
     gene            complement(4415..4861)
                     /locus_tag="VN178_15725"
     CDS             complement(4415..4861)
                     /locus_tag="VN178_15725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWS82467.1"
                     /translation="MSDDTIALSDEQLDQLARYFNEEVTFSRHIGAKVEEVEPGRSAI
                     AIDVKDIHLNGAGTLHGGVYASLIDTAMGLSVLALVGVRTATIEMNVHFLGAVSEGRI
                     TCHSEVVHRTRRTATAEAKVRDSEGTLVAMGTGAFRVFERKGSAIV"
     gene            6281..6475
                     /locus_tag="VN178_15730"
     CDS             6281..6475
                     /locus_tag="VN178_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82468.1"
                     /translation="MFVHGELWLAVLAFAPVETDPRDGEPIAGTGRRVRLLGFGEGGV
                     MQVVPIGPPGSGRDLDQPGR"
     gene            6489..>7087
                     /locus_tag="VN178_15735"
     CDS             6489..>7087
                     /locus_tag="VN178_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWS82469.1"
                     /translation="MYATLGLLQAVGVSFAFIALVVVVVVVASFLYSAVKVVKEYERG
                     VIFRLGRVQGGPKGPGLFLLLPLVDRMVKIDLRTVTMDVPPQDVITRDNVPARVNAVI
                     YFRVVDPNRSVIEVENHVLATSQISQTTLRSVLGQKDLDQLLTNREEINEELQRIIDE
                     QTDPWGVKVSVVEVKDVEIPQQMQRAMARQAESERERRA"
BASE COUNT         1379 a         2081 c         2297 g         1330 t
ORIGIN      
        1 ccgccggcga cgaggagatt cgctgcataa tccttctggg cgaaggtcgg ggcttctccg
       61 ccggcgccga cctggcgcag atcatggaga gcgcggacgg agaccccgac ctgggagaat
      121 acctgcgcac cacctacagc cggctcgtaa agcggatggt cgggatccag aaacccatag
      181 tcgccgccct gcacgggccc gtctacggcg caggggttgg cctggcgctc gcgtgcgacc
      241 tgcgcgtcgc ggcgaagaat gcgaagttct cggtggcctt tatcaagata gggctcatgc
      301 cagatgcggg ggccaccttc ttgctgcccc gcgtcgtcgg cctcggaaag gctatggaga
      361 tgagcatgct cggcgacgcc gtagacgctg aagaagcgct caggatagga ctcgtcaaca
      421 aggtggtagc cgacgattcg ctctcggaag aggtgcggaa cctggccggg cgtcttgcca
      481 cgatgccgac gagtgccctt ggcaggatga agcacgccct ctatgccagc ttcgagaggg
      541 accttgaaac ggccctggag gccgaggccg agggccagac gttctgcggc tatacccaag
      601 accacaaaga aggcgtaaca gcgttcttcg agaagcgcga ggcgcgattc accggcctgt
      661 aggggcgggt ttcaaacccg tcccagggtt caaacccgtc cctacctccc taccattctt
      721 cgagctcggg cctgcgtctg ccgggcagcg agtagaacaa ggctccgtcc ctgctctcca
      781 ccgcgaggtg cccgcctccc gcgagctcgg acagcatccc gtcagcctcg cgcacggtga
      841 gcgaggtctc catagctgcc tccgcgggcg ttatgctgcc gccgttggcc ttgatcgctg
      901 agagtagctc ccgctccttg ttggcgggct gcggcaggac gggtcgttgt ttctgaaagt
      961 cctttagggc cagcccgaga aaaatggccg ccgggatcac gaagccgacg cccgctgcgg
     1021 gtactcctgc ggtggcaatg accattatcg cggccaccac gaatccgacc ccaagagtca
     1081 ggtacaggta cccgttgccc ccgccgggct tcgatacctc ccctctacgc cgctctagat
     1141 cactcaccac gcctccgatt ttcccaagct ccgagtcatt cgcgttagag aactcgtgct
     1201 ttcgacttca ttctagtctc tgccatccac ggtgcaagcg gagcccataa ggttcctacc
     1261 tgcgtgtttt tgtagaatgg tgcgggcttt cggacgagac gggggtagac gatattttga
     1321 agaggctcag ggtgctgatg acacgacagg agatcgtgcc agagaggctt gctggcgcca
     1381 ccgccgtcgt catcgacgtg ttcatggcca caacgaccct ggtgaccatc ctggaaaacg
     1441 gcgcaaggga catctacccg gtcgcgagcc tggaagaggc cgacgaggtc ggggcgaagc
     1501 tggacgccgc gggcgtcttg cgcggcggcg agcaggacgc ggcgcggata gagggctacg
     1561 atcacggtcc cttcccggaa gagtacgcgc ccgaggtcgt cgggggcaaa gacgtgatct
     1621 tcgtgaccac caacggcacc cgcgccatcg ccgacgtagc cccggcggac acggtactcc
     1681 tcggaaccct ccgcaacgct ggggccgtag caaaccacct cgaagcctcg gggacggact
     1741 ccatctatct cgtctgcgcg ggcgcagggg ggcgcttcaa cgtcgaggat ttcctcggcg
     1801 cggcgacggt cctctcgcgt atggatgtgg acggctatcg cttgaacgac ggcgccttgc
     1861 tggctctcga cttcgccgaa cgccacgaag aggacgtgta cgagacgctg aaacgcagcc
     1921 gggcgggtcg atggttcgtc gagaacgaca ggatggacgc cttcgacttc gtcgccgaca
     1981 tgggcgcgag cgggctggtc cccgaggtag cgggtggcaa gctccggcgc gccaacgagc
     2041 aagaaaggta cgtcggggac gccgagcgga gcctgggagg gtggacatga gcgagacgat
     2101 agagattcgg gaaggcgagg acttcgacct cgaagcaata gagcgatatc tgcgggcaca
     2161 cgtagaagac gtgccagaag gtgaactcga agtcagtcag ttcccctcag gcgcctccaa
     2221 cctgacctac ctcttgcggg tgggggattg ggagggcgtt ctgcgccgcc cgccgctcgg
     2281 cccggtcccg ccgaaggcgc acgacatggg ccgggagtcg ggcatcctgg ccaggctcaa
     2341 cgccgtctat ccactcgccc cgaaaccata cttcttctgc gaggatgagt ccgtcatagg
     2401 ggctcccttc tacgtgatgg aacggagaaa ggggatcgtg atcgacgaca ccttccccga
     2461 tggcgtagat aacgacgagg agctgtgcca ggggatctcg cgcaccctcc tggatacact
     2521 cgtcgaactg cacgcggtag acgtggggaa ggcgggactg ggggaccttg gcaagcccga
     2581 gggttttctg cggcggcaga cggaaggttg gatctcacgc tacgaagagg cgaagaccga
     2641 cgagatagaa gaggttggcc ccctcacgaa ctggctggcc cgcgatatcc cagagagccc
     2701 atccccggcc gttatccaca acgactacaa gctcaacaac ctggtcctgg acccggaaga
     2761 tcttacgagt gtacgggccg tcctcgactg ggagatggcg accgtcgggg acccgctctt
     2821 cgacctcgcc gtctcgctct cttactggat cgaacccggc gaccctgacg agcttgcggc
     2881 cgtcttgccg accgtgacgg tgacgccagg cttcatgacc cgcagagagc tcatcgaccg
     2941 ctacgaaaga gagagtgggc gcgacctctc cgagatgcac tggtacgtcg tcttcggata
     3001 cttcaagctg gccgggattc tgcagcagat ctacgcccgc tacaagaacg gccagaccac
     3061 agatgagcgc ttcgccactt tcggcgagcg ggtaagaacc ttgatcctgc acgccgaaaa
     3121 cctctcccgc acgggcgaag tctgatgccc ggcttcgccg ggcagcattt cagcaaggcg
     3181 gctgcgccgc cttgtcagca tttcagctag tcagcttgtc tgcggaaggc aatgagagga
     3241 tttgtttgaa gtagcggttt atgaaggagt gattcgtgat cgagcacagg cgaggaaccg
     3301 ataccaggaa agctgaattg ctgaaagcga agcccgaagg gcgaagcgtg ctgacaagcg
     3361 gaggcgaagc cggagcgtgc tgaccagcta gcgacgaccg aagggaggag cgaacttggg
     3421 ccgtctcgac gacagggttg cgatggtgac gggggcgtca cgcggtatag gtgaggccac
     3481 cgcgcgccgc ttcgcgcggg agggtgcgcg ggtatgcctt acggacgtga agttggaggg
     3541 tgcgctctcc acggcgcgcc agctcgtgaa tgagggtatg gacgccttcg cggccagagt
     3601 cgacgtcacg agccgcaccg aagtcgagaa cgcagtaaag gagatagtcg accgctacgg
     3661 gcggctcgac atcctcgtca acaacgccgg cgtaacgagg gataacctct tctacaagat
     3721 gacggacgag gactggcgca ccgtgttgaa cgtccacctg ggcggcgcgt tcttgtgcac
     3781 cagggccgcc cagaggcaca tggtcgagag gaactacggc agaatcgtca atgtctcgtc
     3841 gacgagcgcg ctcggcaaca gcggccagac caactactcg gcggcgaagg cgggcataca
     3901 gggcttcacc aagaccctcg ccatcgagct gggtcgctac ggcgtaaccg tgaacgccgt
     3961 agcccctggc tttatagaga cggagatgac acgcgagacc gctgccaggc tcggcgtgga
     4021 cttcgacgac ttcgtcgccg agcgcgtaca gagcatcccc gccggccgcg ccggatggcc
     4081 cgaggacgtg gccgccgcca tcctgtacct tgcctccgag gaggccgcgt tcgtgagcgg
     4141 ccaggtgctc tacgtcgccg gcgggccgac agattaaagg gttgcatcac gaggagaaac
     4201 gatggaagaa actgtaggaa gcctggacaa agaacagttg acccgcctcg ctcagtactt
     4261 cgacgagaag gtcatatcag tcggagatta caaaaccaac cgaggtgagg ttccggactt
     4321 tccttaatgg ggcaggcatc gagcgcttgg aattgatgag ggccgattta tcggttgctt
     4381 gacggcttgc agatcggcga cgaaaggcca cggctcaaac gatcgcgctg cccttcctct
     4441 cgaacacgcg gaacgcgccg gtccccatgg cgacgagggt gccctccgag tcgcgtacct
     4501 tcgcctcggc ggtcgccgtc cggcgggtgc gatgcaccac ctcgctgtgg cacgttatgc
     4561 ggccctcgct taccgccccg aggaagtgca cgttcatctc tatggtcgcg gttctcacgc
     4621 ccacgagggc caacaccgat aggcccatag cggtatctat tagggaggcg tacaccccgc
     4681 cgtggagcgt gcctgccccg ttgaggtgga tgtccttgac gtctatggct atggccgagc
     4741 gtcccggctc gacctcctcg accttggccc ctatgtgtct cgagaaggtg acctcctcgt
     4801 tgaaatatcg tgcgagttgg tccagctgct catcgctcag ggctatcgta tcgtcgctca
     4861 ccgggcgctc ccttctattt cggcacttca ggccgctggg tccattcagg ccacgaacct
     4921 tctaccgaag cggattccca ggtgaggtcg agtgggaata tgagcttggg gcttcttgct
     4981 tccactcgcc tcctgagaag caggtacatt ggcgactctg acagtcggca gtttacactt
     5041 cgatgtctcg ctgatcgagc gcggtgcttg ggtaatcttc accttaccgc gatcgatcta
     5101 caggagggtc cgtgctccac tttcgctgaa acaagttgtt agagagcttc tcatcggata
     5161 atgatccacg gggatcatta tccgattcgt acgtggttgg agacccgtgt ccgagtgaag
     5221 cctgtcatcg tcgttcagga tagtcaggcg cagatagtgg gttccatcgt gttgatgctg
     5281 atgctttcga agaattttct gcctcaaggg tcccttctca gattgcgaca tttccgtagc
     5341 tgtgttgctc gttacgaatc cccgaagttg aaagaacgcc cgcgctcggc ttccgtgcct
     5401 tgctgaacga gagcggtctc gagcggttgg aattgacgcg agcgtaaggg gccttgagag
     5461 cacgtatgca gaatggcacg gatacgctcg catcgatgca caactgcata aatacggagg
     5521 tcaggtttcg cgctttactg acccaggatg agattcggca actggaagtg gtgcctgcca
     5581 acatgaatag tgggggcgcc atgcgtgtct tgctacatcg atgagtagga gcgacaatca
     5641 gccgtccctt cagcgtggct tccgtgctgt tcggtccgct cgtcggctct ccaaaatcgt
     5701 cttatatgag ccgaatattc ggaataagga gagtcctcgg tttcgccgaa cgacggtccc
     5761 tactatctag atggcccttt gggtggcttc gaatggttct agcatagggt gtcgggcccc
     5821 cgtaaaggta gagagcgacg acctcgcaag aatctctcat ggcgatgtgc ggtacaatca
     5881 cctcagaccg acgatgctgc ccttactagc atacctatct cccgaccagg cagcgcgtta
     5941 ccgacggtga caacgtctgc gatcggagcc cctctgatac ttgttgggat cgtgcttttc
     6001 gtcatggtcc tgcaccttat cggccatggc ctgctcgccg cagggggtat cggagcgttg
     6061 ctcgccggaa gcctgctact gctgtgggct ggcatcccct actcgggcgt gttgctcggc
     6121 gttcttgctg tcatcgcggt gctcatggga ggcgtgctct tcggggtggt aggttcgttg
     6181 cgcacgctcg taaggaggcc tgcccttacc ggcaaggaag ggatgatagg cgaggtcggc
     6241 accgtctgta gccctgtggg agcggactcc gacggggtgg gtgttcgttc acggggaact
     6301 gtggctcgcc gtgctagcgt tcgctccagt ggagaccgat ccgcgagacg gcgaacccat
     6361 cgcggggacg ggccgcaggg tgaggctctt gggcttcggc gagggaggag taatgcaggt
     6421 ggtgcccatc ggaccgcccg gtagcggtcg agatctcgac cagccgggcc gatagaggag
     6481 gatttgtcgt gtacgcaacc ttgggcttgt tgcaggcggt gggcgtgagc ttcgcgttca
     6541 tagcgctcgt ggtggtcgtt gtagttgtag cctcgttctt gtacagcgcc gtgaaggtcg
     6601 taaaggagta cgagcggggc gtcatcttcc gcctcgggcg ggtgcagggc ggacctaagg
     6661 gacctgggct gttcctgctg ctgccgttgg tggacaggat ggtcaagatc gatctgagga
     6721 ccgtgaccat ggacgtgccc ccccaggacg ttataacccg cgataacgtc cccgccaggg
     6781 tcaacgccgt catctatttc agggtagtgg accccaacag gagcgtcatc gaggtcgaga
     6841 accatgtgct ggccaccagc cagataagcc agaccaccct aaggagcgtc ctcggccaga
     6901 aggacctcga tcagctcctt acaaacaggg aggagataaa cgaagagctg cagagaatca
     6961 tagacgagca gaccgacccc tggggggtga aggtcagcgt ggtggaggtg aaggacgtgg
     7021 agatcccgca gcagatgcag cgggcgatgg ctcgtcaggc cgagtcagag cgcgagcgtc
     7081 gggcgaa
//
LOCUS       DATMVF010000300         8899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_458990, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000300 DATMVF010000000
VERSION     DATMVF010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458990"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VN178_15740"
     CDS             <1..567
                     /locus_tag="VN178_15740"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWS82470.1"
                     /translation="HRGAIWALGLLTAGAAMAPPEASPTDITALAGRVAAFPDRYAPT
                     GASHGSLAAVRYGVSGARGEARQGFPHVVSVALPALRGARRAGCSEEFARLDSLVALI
                     AHVDDTCLLHRGGRSALRDAQSGARAVLDVGGTATAAGRRALRSLEAAMLAYNAAPGG
                     SADLLAAALFLDALEDRVAADSIHEEVA"
     gene            569..862
                     /locus_tag="VN178_15745"
     CDS             569..862
                     /locus_tag="VN178_15745"
                     /inference="COORDINATES: protein motif:HMM:NF002293.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HWS82471.1"
                     /translation="METLTFDYPGSQPVAHHAHVGVVGSGDLEILLGPSPDQQAHVIV
                     RTSVDGFGEVWEAVLDRFFTRNDLAAAVEINDSGATPGTVALRLAQAVEESRS"
     gene            859..1710
                     /locus_tag="VN178_15750"
     CDS             859..1710
                     /locus_tag="VN178_15750"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR03133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HWS82472.1"
                     /translation="MTFPARPKTRLTELSARERAAALLDSGTFRELIGPFERLESPHL
                     ELQGVVPACDDGVVVARGDLDGKEAVVLAVEGLFQGGGIGEVSGAKIAGALELALRDA
                     ESGHPVCPVLLLETGGIRLQEANLGLLAVAEIHQAIVALRSHVPVVGVVAGMVGCFGG
                     MAIAAGLCSHVIMTREGRLGLNGPQVVEQEAGVAELDSDDRRLVWETIGGQQRVATGV
                     ADVLVEDDVAMIAETVRTACEAGVPAENRSAQVGRYEARLARIDVSEPVDGPALRALW
                     DDEGEER"
     gene            1707..2510
                     /gene="mdcE"
                     /locus_tag="VN178_15755"
     CDS             1707..2510
                     /gene="mdcE"
                     /locus_tag="VN178_15755"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR03134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HWS82473.1"
                     /translation="MSRGKTWFEALAGAIPDGEPASVLCADGTVGDERSRFLAVVPNP
                     EARFPRARDGELGLEEGWALARRVREAVVEDLDDDRRALVAVVDVPGQPYGYREELLG
                     LHLALAAAVDAYATARLAGHPVVALVVGQAISGAFLAHGMQANRILALEDPGVAVQVM
                     SKRSTARVTRRSVEDLDEIAKEVPATAYDVTSFARLGALHRLIEGVNAEQPDAEDVER
                     VREALAEAIYSARHDGSTDLSGRLASPEARQHRDASLRVRERLAEEWSR"
     gene            2507..3145
                     /locus_tag="VN178_15760"
     CDS             2507..3145
                     /locus_tag="VN178_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HWS82474.1"
                     /translation="MRPHDLLRLTGGAGLCHEESVPAWVPTSLARAPWVVVRRARGPA
                     GLIPVGVRGSTRAERFAALLTPDAVAARVTPEDLIAERVWHNMDRARWIRSLRVLDAV
                     DELFAALGFAWGPTGSVGFELATGVAAASTASDLDVVVRAPEPLPLGNAREIADYLDR
                     LPVRVDVQLDASAGAVTLADYVRGGRILLRTPDGPILTRDPWREAATNLRSA"
     gene            3142..4086
                     /gene="mdcH"
                     /locus_tag="VN178_15765"
     CDS             3142..4086
                     /gene="mdcH"
                     /locus_tag="VN178_15765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
                     /protein_id="HWS82475.1"
                     /translation="MSVAFLFPGQGSQCPGMLHDLPDHPVARDMIAHATEVLGGEVLD
                     LDTPEALDSTGATQLALLVVGATAARILIEEGGAPDFVAGHSVGAFAAAVAAGALAYP
                     DALRLVDLRARAMQEAYPAGYGMGVVAGLDERTVSRLTAESGTPNAPVHAANVNAPLQ
                     VCVAGADEALERVLDLAREHGAHRVRRLAVPTPSHTPLMAPVAAKLERVLAAVSMVPP
                     AVPYLSNVGGRALRDPEEIRDDLAQSVEWPVRWHDATTLLFELGVRLFVELPPGRVLT
                     DLATVAFPEARAIAVADAGIGSAVTLIARHRPDRRALR"
     gene            4497..5507
                     /gene="dctP"
                     /locus_tag="VN178_15770"
     CDS             4497..5507
                     /gene="dctP"
                     /locus_tag="VN178_15770"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HWS82476.1"
                     /translation="MLVGLLSACGLAESGGGAGAVTLRLSHQWPGASEGEGDFRAVLA
                     QRFAQQVKERTNGDVTIKIYPNNSLIEDPEQQYQAMEQGTTDMSVYPLDYASGDVPEF
                     SVTLMPAMVRNHAQAQNWQDAEIGRKVEEMTEENGIKILTWVWNAGAIGVKEGSAVVS
                     PDDVRSGMVTRAAGPRVEQMLERVGFGLASMDSSEIYNAMQTGTLDSAITSTSSFSSY
                     RLYEQVNSYTSPTGGNTFWFMFEPLIISMEEFEQLTPEQQKIFEEEGAELQEYAYKAS
                     QEDDVRVDKEFEEAGVKVVPMDDRSFVEWQQVSQPVWDDFAADVKGGQQLIELASNVP
                     DN"
     gene            5574..6137
                     /locus_tag="VN178_15775"
     CDS             5574..6137
                     /locus_tag="VN178_15775"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HWS82477.1"
                     /translation="MSGPRNPRSPLLLRYIDRLSEFAGYASAVLILLSMLIVCYGVLL
                     RYVFGASTVWQLELSTYFLMFSAFVGGAYGLKHGDHVNLSLIVDRLPEKPRRYVQLVA
                     SVLGFVFVVTVAAIAFVLWWETTQEGMTSGTAWNVPLTYPYLIVPLGMTLIALQYLVI
                     VVHILQRIRTGDQPANEGEDLEGGRTQ"
     gene            6134..7498
                     /locus_tag="VN178_15780"
     CDS             6134..7498
                     /locus_tag="VN178_15780"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HWS82478.1"
                     /translation="MSALTIGAIVCLILLVTLAIGIPVAFALGLTAILSIVIFLTPEE
                     FAFFADFVFASLNDFALLAIPLFVLMGAIFGHSKASQDLLEVAHVWVGRLPGGLAMSS
                     VMACSVFAALTGSSPATSAAIGRIAIPEMTKRGYPNGLATGAIVAGGTLGILIPPSVT
                     LILYGIATETSIGRLFLGGVVPGIMVTLLFCLWILVVVFSNRRSARVQQQTGSTGEGS
                     GASQYVDEEERYSWKHRFGLLIKVIPFVVLIACVLGVLYLGIATPSEAAGVGALLALI
                     LVALLYRSVNVRNLMDIALETTRTSTMILMIVAFSAVLGQILSFLNVPQELAQLVADL
                     QVNRWVVFVIMNLLFLFLGFFIPPVAIILVTMPVLFPIITELGFDPIWFGVVMTLNME
                     MGLITPPVGLNLYVVQGIAPDIPLRDILAGSLPYVGVLALGILILALFPGLVTWLPNQ
                     TLGG"
     gene            7499..8119
                     /locus_tag="VN178_15785"
     CDS             7499..8119
                     /locus_tag="VN178_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWS82479.1"
                     /translation="MLRIWGRSNSINVQKVLWCCEELDLRYQRVDVGGPFGGNKEPEY
                     LQLNPNGLVPTISDGGFVLWESNVIVRYLAAKHGMGTLCPEDLAERADADRWMDWQMG
                     TLWANFRPAFVGLIRTPPEKRDRDFIATAISKTTENLAMLDAHLATRDYVTGPAFTMA
                     DIPPGVTAYRWFSLEIERPPMANLEAWYERLRARSSYKATVMSPLS"
     gene            complement(8383..8673)
                     /locus_tag="VN178_15790"
     CDS             complement(8383..8673)
                     /locus_tag="VN178_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82480.1"
                     /translation="MYARVTNIRFPLEMEAEVTGVAQGLVPILRRQRGFEGLQVLTDP
                     NVGEGIIVCFWETEAEALASEATSSYIGQMSMMSSFLHEPLVPKTYEVSVKV"
BASE COUNT         1544 a         2784 c         2893 g         1678 t
ORIGIN      
        1 caccgtggcg cgatttgggc cctgggcctg ctcaccgccg gcgcggctat ggcaccgccc
       61 gaagcctccc caacggacat cacggcgctc gccgggcggg tggcggcttt ccctgatcgg
      121 tacgccccta ccggggcgtc tcacggttcc ctggccgcgg tgcgttacgg cgtgtctggg
      181 gcacggggag aggcgaggca aggtttcccg cacgtcgtca gtgtcgcgct gccggccttg
      241 cgaggtgccc gtagagcggg atgctccgag gagttcgccc gcctggactc gctcgtcgcc
      301 ctcatcgccc atgtagacga cacgtgttta ctgcaccgcg gtggccgctc ggccttgcgg
      361 gacgcccaaa gcggagcccg agcggtgctc gacgttggcg gtaccgccac cgcggcgggc
      421 cgtcgtgcgc tgcggtcgct ggaagcggcg atgctagcgt acaacgccgc gcctggcggt
      481 agcgcagatc tgcttgcggc ggctctgttt ctagacgccc tggaggatcg agtcgccgcc
      541 gactcaatcc acgaggaggt agcttgaatt ggaaacactg accttcgact atccgggctc
      601 tcagcccgta gcgcatcacg cgcacgttgg cgtcgtcggc tctggtgacc tggagatcct
      661 gctcggtcca tcgccagatc aacaggccca cgttatcgtc cgcaccagcg ttgatggatt
      721 cggggaggtc tgggaggcgg tgctcgaccg cttcttcacc cgtaatgacc tcgccgccgc
      781 cgtcgagatc aacgactccg gtgccacgcc cggcaccgtc gccctcaggc tggcccaggc
      841 cgtggaagag agccgttcgt gacctttccg gcccgaccga agacgaggtt aacagagctc
      901 tccgcccgcg agagggcggc cgcgctgctg gactccggca ccttccgaga gttgatcggt
      961 ccgttcgagc ggctagaatc cccgcacctg gaactgcagg gcgtcgtccc cgcgtgcgac
     1021 gacggggtcg tggtggcccg cggcgacctg gacggcaagg aggctgtcgt gctcgccgtc
     1081 gaaggcctct tccagggagg aggcatcggg gaggtatccg gcgcgaagat agccggcgcg
     1141 ctggaactgg cgttgcgtga cgccgagagc ggccatcctg tctgccccgt gctgctcttg
     1201 gagacaggcg gcatccgact gcaagaggcc aacctcgggc tgctcgctgt ggctgagata
     1261 caccaggcga tcgtggcttt gcgctcccac gtcccggtgg tcggcgtggt cgcgggcatg
     1321 gtcggctgct tcggtggcat ggcgatcgcc gccggtctgt gcagccatgt catcatgacc
     1381 cgggagggcc ggctaggcct caacggtccg caggtcgtgg agcaggaggc cggagtcgcg
     1441 gagctcgact cagacgaccg ccgcctggtg tgggagacca tcggtggtca gcagcgcgtc
     1501 gccaccggcg tggccgacgt gctcgtcgag gacgacgtcg ccatgatcgc cgagacggtc
     1561 cgaaccgcgt gcgaggcagg agtgcctgcg gagaaccgca gcgcgcaggt cgggcgctac
     1621 gaagcccggc tggccaggat cgacgtgtcg gagcctgtcg acggaccggc gttgcgtgcc
     1681 ctctgggacg atgaggggga agaacgatga gcaggggcaa gacctggttc gaggcgctgg
     1741 ccggagcaat ccccgacggt gaacccgcat ccgtgctgtg cgcagacggc accgtgggcg
     1801 acgagcgatc gcggtttctc gccgtcgtcc ccaatccgga ggcgaggttc cctcgcgcgc
     1861 gtgatggcga gctgggccta gaagagggtt gggcgctcgc ccggcgcgtg cgcgaagctg
     1921 tggtcgagga cctcgacgac gaccgtcggg ccctcgtcgc cgtcgtcgac gtgcctggcc
     1981 agccctacgg gtaccgcgag gagctgttgg gactccacct cgcgctcgcg gcggcggtcg
     2041 acgcctacgc tactgcccgg ctggccggac acccggtcgt ggcgctcgta gtcgggcagg
     2101 cgatctccgg ggcgttcctc gcgcacggca tgcaagccaa ccgtatcctt gcgctcgagg
     2161 atcccggcgt cgccgttcag gtaatgtcca agcgctcgac cgcccgcgta acccgccgta
     2221 gcgtggagga tctggacgag atcgccaaag aagtacccgc caccgcatat gatgtcacct
     2281 ccttcgcccg actgggagct ctgcaccgcc tcatcgaggg agttaacgcc gagcagccag
     2341 acgcggagga cgtcgagagg gtgcgagagg cgctcgccga ggcgatctac tcggcccgcc
     2401 acgacggctc tactgatcta tcgggccggc tcgcttcccc agaggcccgc caacaccgag
     2461 acgcgagctt gcgggtccgg gagcggctcg ctgaggagtg gagcaggtga ggccgcacga
     2521 cctgctgcgt ctcactggtg gcgccgggct gtgccacgag gaatccgtcc ctgcctgggt
     2581 gcccacgtcg cttgcccggg cgccttgggt cgtggtgcgc cgggcacgtg ggccggctgg
     2641 cctcatccct gtcggggtgc gagggagcac ccgtgcggaa cggttcgccg ccttgctcac
     2701 gccggacgcc gtcgcggcgc gtgttacccc ggaagacctc atcgcggagc gggtctggca
     2761 caacatggac cgcgcgcgct ggataaggtc cttacgggtc ctggacgcgg tcgacgaatt
     2821 gttcgccgcc ctaggcttcg cctggggccc gaccggtagt gtcggcttcg aattagccac
     2881 aggcgttgcc gcagcgagca ccgctagcga cctcgacgtg gtcgtccggg cgccggagcc
     2941 cttgccgctc ggaaatgcac gggagatagc agactatctg gacaggttgc cggtgcgtgt
     3001 cgacgtgcaa ctcgacgcgt ccgccggggc agtgacgctg gccgattacg tccgcggcgg
     3061 gcggatcttg ctgcgcacgc ctgacggacc gatactgacc cgcgacccgt ggcgagaagc
     3121 cgcgactaat ctgcggagcg catgagcgtc gcgttcctgt ttcccggcca aggctcgcag
     3181 tgtcccggta tgctgcatga cctgccagat cacccggtag cccgcgacat gatcgcacat
     3241 gcgaccgagg tactcggggg tgaagtgctc gacttagaca cccctgaggc tctcgactcg
     3301 accggcgcca ctcagctcgc cctgctcgtt gtcggcgcta ctgccgctcg gatactgatc
     3361 gaagaaggtg gcgcaccgga cttcgtggca ggacactcgg tgggcgcctt cgccgcagct
     3421 gttgcggccg gcgcgcttgc ctacccggat gcgctgcgcc tggtcgatct gcgcgcccgt
     3481 gccatgcaag aggcgtaccc ggcaggatac ggcatgggcg tcgtcgccgg gctggacgag
     3541 cggacagtat cccgtctcac agcagaatcc ggtaccccga acgcgccggt tcacgcggcc
     3601 aacgtcaacg cgcccctgca ggtgtgcgtt gccggcgcgg atgaagccct ggagcgggtt
     3661 ctggacctcg ctcgcgagca cggcgctcac cgtgtccggc gcctcgccgt gcccacacct
     3721 tcccacacgc ccctcatggc cccggtcgcg gcgaaactcg agagggtcct cgctgccgtt
     3781 tccatggtcc cgcccgccgt accgtacctg agcaacgtcg gtggccgcgc gctgcgcgac
     3841 ccggaggaga tccgtgacga cctagcccag agcgtcgaat ggccggtccg ctggcacgac
     3901 gctactacct tgctcttcga gctcggtgtc cgactgttcg tcgagctgcc gcccggccgc
     3961 gtgctcaccg atctggcaac tgtcgccttt cccgaggctc gtgccatcgc tgtcgccgat
     4021 gctggcattg gctcggccgt caccctcatc gcccgccacc gacctgatcg ccgcgcactg
     4081 agatgatgcg cgaaggatat gtcactgctt atctgccgag cgaaccacag tccggacacc
     4141 aagtcgccga tcgcgaaggt cgcacggggc cgatcgccgg ccgtgaaagt gatctgccga
     4201 tgcgtgcccg tgcttccgca ccgagagctc ctcctggtca aacgtgaccg aaaaatcttg
     4261 ctatgctagt aacattcctt ggtcggtcga cattcgtctg gggaaaggac caagagagaa
     4321 atagaggagg gtattgcgta agcgacgcag ccaggctact tataccttgg cgagtttgcc
     4381 gcaggagtgt ggatgatctg gtagcactgg ggatttcgat aacggctgcc aaagaacgag
     4441 ggaaagggaa gatgacgacg ctaagacggc gctttttgtg cgctatagga gcggtcttgt
     4501 tggttggctt gctgagtgcc tgcgggctcg cggagagcgg aggaggagcc ggtgcggtaa
     4561 cgttgcgcct gtcccaccag tggccaggcg cgagcgaagg cgagggtgac ttccgtgccg
     4621 tactggcgca aaggttcgcg cagcaggtaa aggagagaac aaacggagac gtgaccatca
     4681 agatctaccc caacaactct ctgatagagg atcccgagca acagtatcag gcgatggagc
     4741 aggggaccac ggatatgtcg gtctacccgc tcgactatgc ctcgggcgac gtgccagagt
     4801 tcagcgtcac cttgatgcca gcgatggtac gcaaccacgc tcaggcgcag aactggcaag
     4861 acgcggaaat cgggcgcaag gtcgaggaga tgacggagga gaacgggatc aagatcctca
     4921 cctgggtatg gaacgcagga gcaatcgggg tcaaggaagg gtccgccgta gtctcgccgg
     4981 acgatgtccg gtctggcatg gtgacccgtg cagcgggtcc cagggtagag caaatgctcg
     5041 agagagtagg cttcgggctc gcgagcatgg actcatcgga gatctacaac gctatgcaga
     5101 ccggaaccct ggacagcgcg ataacgtcaa cttcttcgtt cagttcctac aggctctacg
     5161 agcaggtcaa ttcttacacc tcccccacgg gcgggaacac gttctggttc atgttcgagc
     5221 ccctgataat aagcatggag gagttcgagc aattgacgcc cgaacagcag aagatctttg
     5281 aagaggaagg cgccgaactg caggagtacg cctataaggc ctcgcaggag gacgatgtcc
     5341 gcgttgacaa agagttcgag gaagcaggcg tgaaagtcgt gccgatggac gataggtcct
     5401 tcgtggagtg gcaacaggtt tcccagccgg tctgggacga cttcgccgct gacgtgaagg
     5461 gcggtcaaca gctgatcgag ctcgccagta acgtgccaga caattagggt agtaactagg
     5521 gctggcgcct ccctggcgcc agccctcgaa gtatcgaaga ggggagtatt cgcgtgagcg
     5581 gaccaagaaa cccaaggtct cccttattgc tcaggtacat agatcggctc tccgagtttg
     5641 cgggttacgc gagcgccgta ctgatcctgc tttctatgct aatagtctgc tacggcgtcc
     5701 ttcttcggta tgtgttcggc gcctctaccg tctggcagct ggagctatcg acctacttct
     5761 tgatgttctc cgccttcgtt ggtggcgcat acgggctgaa gcacggcgac catgtaaacc
     5821 tctccctgat cgtcgatcgg cttccagaga agcccaggcg ctacgtgcag ctggtcgcgt
     5881 cggttctggg cttcgtgttc gtcgtcaccg tagccgcgat cgcctttgtc ctgtggtggg
     5941 agaccaccca ggagggtatg acctcgggca cggcgtggaa cgtgccactg acctaccctt
     6001 atctcatcgt gccgctgggc atgacgctaa tcgcgcttca atacctggtc atagtggtcc
     6061 atatccttca gcggatcaga acaggcgacc agccggcgaa cgaaggtgag gatctcgaag
     6121 gaggtcggac tcagtgagcg ctttgaccat aggggccatc gtctgcctga tactgctggt
     6181 gacgctggcc ataggcatcc ctgttgcctt cgcactgggc ctgaccgcga tactctccat
     6241 cgtgatcttc ctcaccccgg aagagttcgc tttctttgcc gacttcgtct tcgcctcgct
     6301 aaacgacttc gcgttgctgg ccatcccgct cttcgtcttg atgggcgcga tcttcggaca
     6361 ctccaaagcg agccaggatc tgctcgaggt tgcgcatgta tgggtgggcc gactccccgg
     6421 cggcctggcc atgagctccg tgatggcgtg ctcggtgttc gcggccttga ccggttcgag
     6481 ccccgcgacc tccgctgcga tcgggaggat agccatcccc gagatgacca agcgaggcta
     6541 cccgaacggg ctggcgacgg gggcgatagt cgcagggggt acgctgggca tactgattcc
     6601 accgagcgtc accttgatcc tgtacggtat agccacggag acgtcgatcg gccgcctgtt
     6661 tctgggcggc gtcgtgccgg gcatcatggt cacgctgctg ttctgtctgt ggattctggt
     6721 agtggtgttc tcgaacagga ggagcgcgcg tgtccagcaa cagaccggca gcacaggaga
     6781 gggtagcgga gcctcacagt atgtcgacga ggaggagagg tactcctgga agcaccgctt
     6841 tggcttgttg atcaaggtga tcccttttgt cgtactgatc gcctgcgtct tgggtgtcct
     6901 gtacctgggc atcgccaccc ctagcgaggc ggcgggcgtt ggcgccctac tggcccttat
     6961 cctggtcgcc ctcttgtatc gctcggtgaa cgtgagaaac ctcatggaca tagctctgga
     7021 aaccacgcgg accagcacga tgatcctgat gatcgtggcc ttctccgcag tgctcggcca
     7081 gatcttgagc ttcctcaacg ttccccagga gctggcgcaa ctggtggccg accttcaggt
     7141 gaaccggtgg gtggtgttcg ttatcatgaa cctgctgttc ctgttcctgg ggttcttcat
     7201 cccgcccgtg gcgatcattc tcgttaccat gccagtcctg ttcccaatca ttacggagct
     7261 cgggttcgac ccgatatggt tcggcgtggt gatgaccctg aacatggaga tgggcctgat
     7321 cacacctccg gtagggctga acctgtacgt ggtgcagggc atcgcgcccg acatcccgct
     7381 gcgggacata ctcgcgggct cgcttcccta cgtgggtgtc ctggcgctcg ggatactcat
     7441 cctcgctctc ttcccaggcc tggtcacgtg gctgcccaac cagacgctcg gaggatgagt
     7501 gcttcggatc tggggccgaa gcaactccat aaacgtccag aaggttctgt ggtgttgcga
     7561 agagctggac cttcgctatc agcgcgttga tgtcggcggt cctttcggcg gcaacaagga
     7621 acctgagtac ctgcagttga accctaacgg tcttgtgccg acgatctctg acggcggctt
     7681 cgtgctctgg gagtccaacg tcatcgtgcg gtacctcgcc gccaagcacg ggatgggcac
     7741 gctgtgcccg gaggacctcg cagaacgggc cgacgcggac aggtggatgg attggcagat
     7801 gggaacgctc tgggccaact tccggccggc gttcgtcggt cttatccgca cgccgccgga
     7861 aaagagggat cgggacttta tagcaaccgc cataagcaag acgaccgaga acctggccat
     7921 gctcgacgca cacttggcta ctcgagatta cgttactgga ccggcgttta ccatggcgga
     7981 cataccgcct ggggtgacgg cataccgctg gttcagccta gagatcgaac gtcctcccat
     8041 ggcaaacctg gaagcctggt atgagcgttt gcgtgctcga tcttcttaca aagcgaccgt
     8101 gatgtcgcca ctctcttgaa aaactgcgac ggcaggtctt cgtaacgtgc gtgctaggag
     8161 cttgggcaaa cgcggaccac acgtggcacg ctctataccg atccactacc tgtgagcagc
     8221 acaggtgaga atctggaacc agcacgaggt gcaccgcgag gccgccgcga gctgggcgtc
     8281 ttggagccgg tggcgcgacg gtgctggtgt gatggtcgag catcgcaagt cgccaagcgt
     8341 cctcgctacc tcactactct tccgatcagc tcggctggct gctcacacct tcacgctcac
     8401 ttcgtaagtc ttcgggacaa gcggctcgtg aaggaagctc gacatcatgc tcatctgccc
     8461 gatatacgag gaggtggcct cgctcgccag cgcctcggcc tctgtctccc aaaagcagac
     8521 gatgatgccc tcgcccacgt tggggtcagt cagcacctga aggccttcga agcccctctg
     8581 ccgcctcaag atcggcacca aaccctgagc aacaccggtc acctccgctt ccatttccaa
     8641 agggaaccgg atgttcgtca cccttgcgta catggttagc ttcctctcgc cgtccctcgg
     8701 tgggccaggt aaccagtcgg taccctgcct tgcatcccat attctaaggt accggagtaa
     8761 gagtcaggtg acctcgcgca atcttccagt ctctacctcg tagatgaagc cgcgtacgct
     8821 ctccttgtgc aagatgaacg ggctcgcctg gattcgagcg atagactggc gcacatcctc
     8881 ctccagatcc gagaagctc
//
LOCUS       DATMVF010000301        14028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_38908, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000301 DATMVF010000000
VERSION     DATMVF010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38908"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..950)
                     /locus_tag="VN178_15795"
     CDS             complement(132..950)
                     /locus_tag="VN178_15795"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWS82481.1"
                     /translation="MTPPEEHYRYREQIGAFLLGKLDGVELTALQSHLNSCPVCQAEE
                     RKLEPVVAALSYAAPDRIDEASWPPEHLEESTLAPILGEMRRARRYGWRFGWSAVAAA
                     AICVVVMGLAGFTWFLEPAVSLELRKDAVRLEISTRGGELVFTAKLPNESRVASENQE
                     GPSPGKNLAPADSSKEEPAASDSGSGIPPVGGSTPESGDKGSSPGGEKPGATSPGTPS
                     PETASPGTTSPKTASPGTASPGTASPGAATEESGPPRGSYGKSPACDQYSCAQE"
     gene            complement(947..1414)
                     /locus_tag="VN178_15800"
     CDS             complement(947..1414)
                     /locus_tag="VN178_15800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWS82482.1"
                     /translation="MLGGELYRMAHRALNDDGLAEEAVQETFLRAWRAADRYDPQRSS
                     LRTWMFAIARNVTIDLARARSARPQRTGLEVDHTHEAKDTTEESLRSWEMEEALRHLG
                     PHHRHAIVETYYRDRPCAEVAAEAGVPVGTMRSRLYYGLKALRAVLEEREWTG"
     gene            complement(1909..2100)
                     /locus_tag="VN178_15805"
     CDS             complement(1909..2100)
                     /locus_tag="VN178_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82483.1"
                     /translation="MKEPMFPKFDYYLDESDPDIVILRRQDGSFVAAFSARGATTEGF
                     VEAAREDYWELVRAHTESA"
     gene            complement(2103..2342)
                     /locus_tag="VN178_15810"
     CDS             complement(2103..2342)
                     /locus_tag="VN178_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82484.1"
                     /translation="MRSQEARVGTRVRVCEHHRISERRGMVGRIVGRYGGEDYVAVDV
                     RFPDRRYRLFWPEDLEEISPPRPWRRSLVGGESRS"
     gene            complement(2713..3423)
                     /locus_tag="VN178_15815"
     CDS             complement(2713..3423)
                     /locus_tag="VN178_15815"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HWS82485.1"
                     /translation="MKFMVASEVFGLFPGLKLPVAVAEGIHLTTEAPGIETMWRQSWE
                     QAARTASDYESPQSHPRVAPWREAMAAMGVSGRKFPSSIEALLRRAFKGGEPPRINPL
                     VDFYNAISLRYVVPAGGFDLGDIDGTLELRLTRQEDTFQPLDGSSEVGVEPGEVAYAS
                     GNEILTRYFVWKQSRKGLLNESTRSLFLVSEVLGVVESDSGVANAVLEDFADGLRRHF
                     DSEPAIFLVGEEDPEVSL"
     gene            3997..5025
                     /locus_tag="VN178_15820"
     CDS             3997..5025
                     /locus_tag="VN178_15820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWS82486.1"
                     /translation="MRTIALEEHYATGAFMEGPGRELKARAEAARDHPRVAAGYEGLI
                     RRLCDLGEGRISEMEAAGVDVQVLSLTSPGVEQLDAGRAVSLAREANETLAEATRRHP
                     GHFAGFAALPTAAPEAAAEELERAVREHGFVGTLINGHTRGRYLDDPFFWPILERAET
                     LGVPIYLHPTPPPQPVVEAYYSGNFAPGVADGLATAAWGWHVETATHVLRIVLSGVFD
                     RFPGLQIIVGHMGEGIPFFVPRLDLALPSEMTGLDRRVGDYLRENVHYTFGGFDWTQA
                     FLDLLLQVGAERIMFSADYPYASMGEARAFLDGLPVGPADREKIAHRNAERLLGLRAA
                     VWAAGCAE"
     gene            5077..5682
                     /locus_tag="VN178_15825"
     CDS             5077..5682
                     /locus_tag="VN178_15825"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWS82487.1"
                     /translation="MESQTDGGPKAGLRERKKARTRAEIQRQALRLFRERGYEATTVA
                     RIAEAAEVSESTFFRYFPTKEDVVLWDEFDPLIFEAFKAQPAESDPIRALRDAIRAVL
                     ARTSPTEREHLRERVGLLLSVPPLRAALVDQLHGPMRLLAVAVAERTGRRPDEPAVRA
                     LAGAMMGVGLSAMFAAVEHSDADIVSLVDEGLAQLEAGLPL"
     gene            complement(6020..7555)
                     /locus_tag="VN178_15830"
     CDS             complement(6020..7555)
                     /locus_tag="VN178_15830"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWS82488.1"
                     /translation="MTVILGPILGFRGLGDGEWRTCALVVTEGNATPPELTWFVDNEG
                     RRNEGGNASERTHLKSFRDFEAWRFDWSVQQSDAEQTIVYTLGDGGEYRFSVPATDQP
                     LRVAYGSCAGFSSLGEMKKVGNKNAMWDVLAQQNRDEPYHLMLLGGDQVYADLVWDTV
                     APLRNRLAGLGGARDWHHAEFTEDVRSAVAEFYFGLYLQRWSQAEPSALLARVPSLMM
                     WDDHDIFDGWGSYPEEQQNSPVYRGIWEQAREHFRLFQLQAADDLDLPSGMLCEPDER
                     TNFTYAYRIGDLALAVLDMRSERTQKRVMSLETWNALQGWMDKELEGCRHLFVVSSIP
                     VVYVNSNLVEAALGFLPGQQDLEDDFKDQWLSRTHKEERLRLIHRLLRFSEKSGCRVT
                     IVSGDVHVAALGYIQSARNGFANEEPNVINQLISSAIVHPPPPGLIVYAMELVMADNV
                     EEVDRGITARMMSFPSITQHFMAKRNWLSLELDEENRVWAKWYVEDKEEPYTKVVHPI
                     DEK"
     gene            7995..9284
                     /locus_tag="VN178_15835"
     CDS             7995..9284
                     /locus_tag="VN178_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWS82489.1"
                     /translation="MTQARYSIRTERSREIAERLERSIPGGNTRSLAYFPPYPLVISS
                     GSGCRIWDADGNEFVDLLNNYTASVHGHAVPAVNEAMGEQAVLGTVFPAPGRLQAELA
                     ERIVGRVASVEKVRFANSGTEAVMMAVRGARAFTGREKIVKAEGGYNGMWEQVPISWP
                     QDPYRAAMPEGVRELVRMVDYNDVGQLEAAMREEGEEVAAIILEPVTGTGVFTGTPEY
                     FAAARRLADEHGALLICDEVITLRLNFGGFQEVLGVRPDLTTMGKIIGGGLPVGAFGG
                     RADVMEVFDPRDRDHLHHSGTFNGNLMTMAAGCVTLDLLTHSEIERINALGERLAEGL
                     RRSLAERPDLHGVVNSCGSLVHVNFETEGEVRRFSDLRLDSPVAAAFHLAALEEGVYF
                     GPRGFMNTSTAMDEQVVDEALEACSRALGRTYPKPDA"
     gene            complement(9295..11985)
                     /locus_tag="VN178_15840"
     CDS             complement(9295..11985)
                     /locus_tag="VN178_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWS82490.1"
                     /translation="MATRMCDVCGVRPAAVAVRRRIPGEGQTVRYLCELHAAEARGGR
                     PPLGASSLGGGSLFDDFFDRFLDEGRTSLPGGGTALRRRAEQVDVTQFFSAATTELLQ
                     RAAQQALEWGSLDLTGEHLLYAALEDTVVRRVLEGAGADPDALRAQLEEEAQKGGRTD
                     VSPTLAPDAKRALLAAYDESRALGASYIGPEHVLLALAADDESDAGRLLSRFGLSHTR
                     LRGAVVRGVEAGGEVREPASSTPTLDEYSRDLTEAAREGKLDPVIGRADEVETTIEIL
                     SRRTKNNPVLIGDPGVGKTAIVEGIAQRVVNDEVPETLAGKRVLQLDLAGLVAGTQYR
                     GQFEERLKKVIEEIREHSEDAIVFIDELHTVVGAGAAEGSMDASNMLKPALARGELRV
                     VGATTIDEYRKNIEKDAALERRFQPVLVGEPSVDDTVEILRGLKDRYEAHHRVKITEE
                     AIVAAAELSDRYITGRFLPDKAIDLIDQAAARVRLRSKTKPVDTRELEDEIGRLKREK
                     DQAVSAEDFEKAQDLKNRIQESENRLEAGQGRRPTAEVTAEDIAEVVSRATGIPVSQL
                     TQEERDRLMRLEEQLHGRVIGQDEAVTAIAEAIRRARAGLGDPNRPIGSFLFLGPTGV
                     GKTELARTLAEALFGDEAAMVRIDMSEFQERHTVSRLVGAPPGYVGYEEAGQLTESVR
                     RRPYSVLLLDEIEKAHPDVFNILLQILDDGRLTDSQGRTVDFKQTVIIMTSNMGAQRI
                     QAHSRRKESFEDLKEDVMKIVRSQLRPEFVNRIDEIIVFRALTREQIADVARMLLERT
                     QRRLHAQDIEVEFTDAAVDLIAEEGYDPEFGARPLRRVIQRRVDNELAGMVLGGSLNP
                     GDKVVAGAEEGLLTLDVMEGAADVPDETDLEGAARETESG"
     gene            12135..13544
                     /locus_tag="VN178_15845"
     CDS             12135..13544
                     /locus_tag="VN178_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWS82491.1"
                     /translation="MTESSIREPHPAPWTGAWDQIDDDFEDHLEEIRTFLRCPTVSAS
                     DDDMLAGAEYVAALIEDAGGSAEILPTAGHPAVLGRIEGDGPRLLRYGMYDVQPADEP
                     GWSSPPFGAFTADVPGAGPSIVARGAANSKGSLASFLLALASARRVSDLPAEVVFLVD
                     GEEELGSPSLPGVMSSRRTDLAAEAAFDMDLTADRRDVAEVYLGCKGILSFVLGCSGG
                     EWGGPVGRALHSSEGVVISSPAWSLARALSALRDAAERDALDGLGAASVPPEDEGLLD
                     ALAREFDPAAHLEESDALAYRGPDDARSILKALMYEPVLNLNGFSSGSVTGKTIVPHE
                     ARAALDIRIPYGTDLESVVTGVERIVAQSAPEVVVEGVQLCPPSRTTADSPVARAMVE
                     SQLDVGPPPQVWPSAPWWAPFYLYEQVLEVTFASGGAGHAGRAHAADEYATIAGLRTH
                     MRQSIAFLHRFARAAEADR"
     gene            13589..>14028
                     /locus_tag="VN178_15850"
     CDS             13589..>14028
                     /locus_tag="VN178_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWS82492.1"
                     /translation="MWTEPLEFGKLTWPEVARAAGERRVPIVPIGTLEDHGYHLPIDT
                     DVTLVEAVCRGAAERLPEETVLLPPIVHGYSPHHMDFPGTVSIQWDTFCRYCTDVALS
                     LVRHGFQRVLMVNGHGSNQNLVETAARLAMVEEPESLVAASFYLV"
BASE COUNT         2309 a         4636 c         4471 g         2612 t
ORIGIN      
        1 gcctccgaac attcaccgtg gctggcaccc cgcgacgatc ttgacgatac gtgggaaaga
       61 gcctgttcgg atccgacctc gggtttccgg aagcagggtc tcgcaagaga gacccgccgc
      121 aaatcggccg ctcactcttg agcacaggag tactggtcgc acgccggcga tttcccatag
      181 gaaccacgag gggggccgct ttcttccgtt gctgccccgg gcgacgccgt cccgggcgag
      241 gctgttccag gcgaagccgt cttaggcgag gtcgtcccag gagacgctgt ctcaggcgag
      301 ggtgttccgg gcgacgttgc tccgggcttc tcgccgccgg gggatgaacc cttgtcgccg
      361 ctctcgggtg tgctcccccc cacggggggt atcccgctcc cagagtcgga ggccgctggt
      421 tcctccttcg acgaatcagc cggagcgaga ttttttccgg ggctcggtcc ctcctggttt
      481 tcagaggcga ccctggactc attgggaagc ttggcggtaa acaccagctc gccgccgcgt
      541 gtgctgatct ccagcctaac cgcgtccttg cgcaactcca gagagactgc gggctccaga
      601 aaccaggtga atccggcgag gcccatcacg acgacgcaga tcgcagccgc ggcaaccgcc
      661 gaccagccga accgccatcc ataacgccgt gcgcggcgca tctcccccaa gatcggcgcg
      721 agggtcgact cttcgaggtg ttcgggaggc cacgaggctt catcgatccg gtcgggagcg
      781 gcgtacgaga gggccgcgac cacgggttcc agcttcctct cttccgcctg gcagacagga
      841 cagctattta ggtgactctg cagggccgtc agctctactc catcgagctt gccgagcagg
      901 aacgcgccga tctgttcccg gtatcggtaa tgttcttcgg gtggagtcat ccggtccact
      961 ctctctcctc cagcaccgcg cgcagcgctt tcagaccgta gtagaggcgg ctgcgcatgg
     1021 tgccaaccgg gacaccggcc tcggcggcga cctccgcgca gggcctgtcg cgatagtagg
     1081 tctctacgat cgcatgacgg tgatgcggac ccaagtgccg cagtgcctcc tccatctccc
     1141 acgagcgaag ggattcctcg gtggtgtcct tcgcctcgtg cgtatggtcc acctccagcc
     1201 ccgtccgctg cgggcgcgcc gaacgtgccc gggccaagtc tatagtgacg ttgcgggcga
     1261 tggcgaacat ccaggtccgc agggagcttc tctgcggatc gtagcggtcg gcggcgcgcc
     1321 acgcccgcag gaaggtctcc tgcaccgcct cttctgccag gccatcatca ttcaatgctc
     1381 tgtgggccat cctgtagagc tcaccgccga gcacgtcgta ggcggcccgc agtccctctt
     1441 cgctgtcgag cgaaggtgcg tccgcccgcc ttcttatgcc gcctgagtcg ctcagttggt
     1501 cggtacgccc cccatgttaa gagccgcttc atttcacgcc gcgttaccgt ctaccaccgc
     1561 cgtagctacc accatagcag ccaccgcggt aatcataacc acggctctcc aagcagaagt
     1621 cgccgccgcc gaaccgaact cggaagccgg ccttcgctcc gccgtccttt cgcggtcggc
     1681 cggcggctcc aagggccaaa tttgcttcct cagcgttcat ctttgttccg gtaagcgtgg
     1741 atctgctcat cactctcttc gccggcggat agcatgctaa ccgttattac gtgcgagagc
     1801 caccgaatgt tcaccggagc tggcaccacg cgacgatctt aacggtcctt cataccagcc
     1861 gcggctttga gtgtggttag cccaagatgc tgtacgccgc ggaactcatc atgcggattc
     1921 cgtgtgagcc cggacgagct cccaataatc ctccctggcg gcttcgacga agccctctgt
     1981 ggtggcaccc cttgcgctga aggccgcaac gaacgagccg tcctggcggc gcaggatcac
     2041 tatgtctggg tccgactcgt cgaggtagta gtcgaacttg ggaaacatgg gctctttcat
     2101 ccctagctcc tgctttcgcc gcccacaagc gagcgccgcc aaggccttgg gggggagatc
     2161 tcctccagat cctccggcca gaacagccga taccgtcggt ccggaaagcg cacgtcgacg
     2221 gccacgtagt cttctccccc gtagcggccc acgatcctac cgaccatgcc ccgccgctcc
     2281 gagatccgat ggtgctcaca cacccttact ctagttccga cgcgcgcttc ttgcgatctc
     2341 atggtgagtt catccccaca acgctctagg aaagctcaga ggcgagcttt tgtcggcggg
     2401 caactccatg ttgcccactc ctgccggaca ggatcggcat ggagggagag ccccacgacc
     2461 tgcaattccg ctatccacct gatcttctcc ctaccttttg atgagcacct taaagggggg
     2521 gaatgccgct gtcatgggac catggtccca acttggttgg gtatagcccg gccgcacgcg
     2581 cgatgtctat tggtcgaact cgagcctggc taagcgaaga gtttgcgatc acgtatcttc
     2641 agagcatccg tgtagtaccc agcccaccag ggttcttctt cgggtcctcc ggtgaaggcc
     2701 ttcatgcgcc agctacagag agacctcagg atcttcttcc ccaacgagga agatcgccgg
     2761 ttccgagtcg aaatggcgcc tcagaccgtc cgcgaagtct tccagtacgg cgtttgccac
     2821 gccgctgtcg gactcgacta ctcccagcac ttctgaaacc aggaacaggg atctggtgga
     2881 ctcgttcagg agccctttgc gggactgctt ccaaacgaag tagcgggtca gaatctcgtt
     2941 acctgaggcg taagccacct cgccgggctc gacgcctacc tcggatgagc cgtccagtgg
     3001 ttgaaaggtg tcttcctggc gggtgagcct caactcgagc gtcccgtcaa tgtctcccag
     3061 atcgaagccc ccggcgggaa cgacgtaccg caaagagatg gcgttgtaga agtccaccag
     3121 cgggttgatg cgcggaggct cgccgccttt gaatgctcgt cgcaggagag cttctatgga
     3181 actggggaat ttcctaccgg agactcccat cgcggccatg gcctcgcgcc agggagcgac
     3241 tcggggatgt gactgtgggc tctcgtaatc cgaggctgtt cgtgccgcct gctcccagga
     3301 ctgtctccac atcgtctcga tacctggtgc ttcagtggta agatggatcc cttccgccac
     3361 ggcaaccggg agcttcaagc caggaaacag cccgaagacc tcggaagcta ccataaactt
     3421 catgatgtac ctgcctttcc gcggacccct cttcggatgc ggcttctccc gtacctcgct
     3481 tattcttcgg gtctcccttc gatgccgccg cggtcgcgac cgtggaatgc ccgcgataac
     3541 gaatcgagac ccctcaacat ccgtacgaac ctggcgcaga ccccacaccg tccgtcgaat
     3601 atgaacagta cagggtaggc tactctcacg cttcgttcac ctgacgccgc taccatacct
     3661 actgtatggt agcggctaat gggcgcttcg gtcggcgcgg gagactccgt ccgaggggtc
     3721 gccctaggcg atctggtgca ccagccgcgg gaagccgtcc ggaccgcccg gcgtcggcag
     3781 cgcccacacg tcgcagagac ggtagtcggc cgcccactaa cggtccgtcc ttcccacacc
     3841 gcttcgccgc acaccctacc gcttacgtcc tcaccctaca tcaagccagc aatcccgcta
     3901 cagcagccag ggtcatcgac ggccggttcc ttgcacaaaa tgatagttca ctctatcata
     3961 atagtagact gtcatatttt tggatcggag tacacgatgc ggacgatagc gctcgaggag
     4021 cactacgcca ccggggcgtt catggaaggg ccgggtcggg agctgaaggc gcgggcggag
     4081 gcggcccggg atcatcctcg ggtggcggct ggctacgagg gtctcatacg gcggctgtgc
     4141 gacctcggcg agggcaggat ctcggagatg gaggcggctg gcgtcgatgt gcaggtgttg
     4201 tcgctgacct ctcctggcgt ggagcagctc gatgccgggc gggcggtgtc gctcgctcgc
     4261 gaggcgaacg agaccctcgc cgaggcgacg aggcgtcatc cgggacattt cgcggggttc
     4321 gcggcgttac cgaccgcggc gcccgaggcc gccgccgagg agttggagcg cgcggtacgt
     4381 gagcacggct tcgtcgggac gctgatcaac gggcacacca gggggcgtta cctggatgat
     4441 ccgttcttct ggccgatcct cgagcgcgcc gagacgctcg gcgtcccgat ctacctccac
     4501 ccgacgccgc cgccccagcc ggtcgttgag gcttactact cggggaactt cgcgccgggg
     4561 gtggccgacg ggttggcgac cgcggcctgg ggctggcacg tcgagaccgc gacccacgtt
     4621 ctgcggatcg tccttagtgg ggtattcgac cggttccccg ggttgcagat catcgtcggg
     4681 cacatgggcg aggggatacc gttcttcgtg ccaaggctgg acctcgccct gccaagcgag
     4741 atgaccgggc tcgaccggcg ggtcggcgac tacctgcgcg agaacgtcca ctacaccttc
     4801 ggcggcttcg actggaccca ggcgtttctc gatttgctgc tccaggtcgg cgccgagcgg
     4861 atcatgttct cggccgatta cccctacgcg tcgatggggg aggcgcgcgc cttcttggac
     4921 ggactgccgg tcggacccgc cgaccgggag aagatcgcgc accgcaacgc ggagcgcctg
     4981 ctggggctgc gagcggcggt gtgggcagcg ggatgcgccg aatgatcggg actctcaggc
     5041 ttggggaggc gggtccgaaa cggctagaat tgttgcatgg agtctcaaac cgatggcggg
     5101 ccgaaggcgg gactgcgcga gcgcaagaag gccaggacgc gcgcggagat ccaacgccag
     5161 gccttgaggc tcttccgcga gcggggctac gaggccacca cggtggcccg gatcgcggag
     5221 gcggccgagg tctccgagag cacctttttc cgctactttc cgacaaagga ggacgtcgtc
     5281 ctctgggacg agttcgaccc actgatcttc gaggcgttca aggcgcagcc tgcggagtct
     5341 gacccgatcc gggccctgcg tgacgcgatc cgcgccgtgc tcgcccggac ctccccgaca
     5401 gagcgcgagc acctgcgaga gcgcgtcggg ctcctccttt cggtcccacc gctgcgtgcg
     5461 gcgctcgtcg atcagttgca cgggccgatg cgcctgctcg cggtggcggt ggccgagaga
     5521 accgggagac ggcccgacga accggccgtg cgcgccctcg cgggggctat gatgggggtg
     5581 gggctatccg cgatgttcgc cgcggtggag cattccgacg cggatatcgt ctccctggta
     5641 gacgaagggt tggcgcagtt ggaggcaggt ctcccgctgt agacacgctc cggcgggcca
     5701 ggttggcgcc cgaacggtcc gtgggccagc ggagctggga cgagttcctc gaagatcgcg
     5761 gtatccccaa cgggaacacg gtgcatctga cggagtagag cgggtctcga gcatgcggac
     5821 ggcagcggta ggcagtgcgc tgttgtcacg acgttcgtca tggacgtagc ggacgatctg
     5881 gtgcggacca ttcgcctggt ggcgaacccg agaagctggc cggcgtcagg ggattcgagc
     5941 aggctcgatg aggatcgacg ctgagctgaa cgagacgatc cgtggctggc gactttcacc
     6001 gtcagagttt tgctgatggt cacttctcgt cgatcgggtg gacgaccttg gtgtagggct
     6061 cttctttgtc ctctacgtac cacttcgccc agacgcggtt ctcctcgtcc agctccagac
     6121 tgagccaatt acgcttcgcc atgaagtgct gagtaatgga ggggaaactc atcatccggg
     6181 cggtgatgcc ccggtcgacc tcctccacgt tgtcggccat taccagttcc atcgcgtaga
     6241 cgatgagtcc tggcggcggt gggtgcacga tcgccgacga gatgagctgg tttatgacgt
     6301 tgggctcctc gttggcaaag ccgttccttg ccgactgtat gtaaccgagc gccgcgacgt
     6361 gcacgtctcc cgagacgatc gtcacgcggc accccgactt ctccgagaac ctcagcagac
     6421 ggtggatgag gcgcagcctt tcctccttgt gcgtgcgact gagccactga tccttgaagt
     6481 cgtcttccag atcctgctgc ccgggcaaaa accctagagc cgcctcgacc aggttggagt
     6541 tgacgtaaac gaccgggatg ctggatacga cgaacaggtg cctgcagcct tcgagctcct
     6601 tgtccatcca cccctgcaaa gcgttccacg tctccagact catcacccgc ttctgggtac
     6661 gctcggagcg catgtcgagc accgcgaggg cgagatctcc gatacggtaa gcgtaggtga
     6721 agttcgtccg ttcgtcgggc tcgcacaaca tccccgaagg aagatcaaga tcgtccgccg
     6781 cctgcaactg gaacaggcgg aagtgttcgc gggcctgctc ccagataccc cggtagaccg
     6841 gactattctg ttgctcttcg gggtaagagc cccacccgtc gaagatgtcg tggtcatccc
     6901 acatcatcaa ggatggtacc cgcgccagca gtgctgaggg ctcggcctga gaccacctct
     6961 ggaggtacag gccgaagtaa aactccgcga ccgccgacct gacgtcctca gtgaattcgg
     7021 cgtggtgcca atcccgcgcg ccgcccaacc cggcgagccg attcctgagc ggcgccaccg
     7081 tgtcccaaac cagatccgcg tatacttgat ctccacccag cagcatcagg tggtaaggct
     7141 catcccggtt ttgctgcgcg agcacatccc acatggcgtt cttgttcccc acctttttca
     7201 tctctccgag gctggagaat ccggcgcagg aaccataggc gacgcgaagc ggctggtccg
     7261 tggcaggtac gctgaagcga tactccccgc cgtcgcccag ggtgtagacg atggtctgct
     7321 ccgcatcact ctgctgaacg ctccaatcga agcgccaggc ttcgaaatcc ctgaatgact
     7381 tcaggtgagt tcgctcggaa gcgttcccac cttcgttccg acgaccttcg ttgtcgacaa
     7441 accacgttag ctccggaggg gtggcgttcc cctcggttac cacgagggcg caggtgcgcc
     7501 attctccatc tcccaatccc ctgaagccca gaatcggtcc gagaatcaca gtcactaccc
     7561 ttattcctcc tccgtaacgt gcggacgaaa gcaggcagac ctgcacgccc taccctctcc
     7621 ttaccggaac ggtcatcgtc accgtttcga gggggtcttc tcaggacccg ccttccagcg
     7681 tccatcatgc cgtacgttcc cggccgccgc aatgcaccag tagggtagcg accggcaacc
     7741 ctagggtttc ctcgccttcc gcgatacgct tctatggctc gacacgggtc ggacactggg
     7801 atttgccctt cggtcgtggt ggtggcaatg caggggacga ctcgggtaga cgctgtggcg
     7861 gagtggggta cggcttagat caaccccgcc aggcgggacg aggatgccat agcatacaat
     7921 cttcacgagc tttactggcg gtctatgatg gtatgtactg gtctaacggc gggacggtgg
     7981 agggcaacgt ggatatgact caagccaggt acagcatcag gaccgaaagg tcgcgcgaga
     8041 tcgcggagcg gctcgagcgt tcgatccccg gcggcaatac ccgttctctc gcctatttcc
     8101 ctccttaccc gttggtgatc tcctccggct ccggctgccg catctgggac gcggatggca
     8161 acgagttcgt cgacctcctg aacaactaca ccgcctcggt ccacggccac gccgtgcctg
     8221 ccgtaaacga ggccatgggc gagcaggcgg tcctgggtac cgtcttccct gcccccggca
     8281 ggctgcaggc cgagctcgcg gaaaggatcg tcggccgggt ggcgtcggta gagaaggtgc
     8341 gcttcgcgaa ctcgggcacc gaggccgtca tgatggccgt gcgcggtgcc agggccttta
     8401 ccggaaggga gaagatagtc aaggccgaag gcggctacaa cggcatgtgg gagcaggtgc
     8461 ccatctcctg gccacaggac ccctacagag ccgcgatgcc cgaaggggtc cgcgagctgg
     8521 tgcgcatggt cgactataac gacgtcggcc agctcgaggc cgcgatgcga gaggaaggcg
     8581 aggaggtggc cgcgataatc ctggagcccg taacggggac gggggttttt accgggaccc
     8641 ccgaatactt cgcggcggcg cgacgcctgg ccgatgagca cggcgcgctc ctcatctgcg
     8701 acgaggtcat aaccctcagg ctcaacttcg gcggcttcca ggaggtcctg ggcgttcgtc
     8761 ccgacctgac gacgatgggt aagatcatcg gcggcggcct ccccgtcggc gctttcggcg
     8821 gacgcgcgga cgtgatggag gtcttcgacc cccgcgatcg agaccacctc caccactcag
     8881 gaaccttcaa cggtaacctc atgaccatgg ccgcgggctg cgtcacgcta gacctcctta
     8941 cgcactcgga gatagagcgc atcaacgccc tgggcgagcg gctcgccgag gggctgcgcc
     9001 gctctttggc cgagaggccg gatctccacg gcgtcgtgaa cagctgcggg tccctggttc
     9061 acgtgaactt cgaaaccgag ggcgaggttc gcaggttctc cgacttgaga ctggacagtc
     9121 ccgtggcggc cgccttccat ctcgcggcgc tcgaggaggg ggtctacttc gggccgcgcg
     9181 gcttcatgaa cacgtcaacg gctatggacg agcaggtagt agacgaagcg ctcgaagcct
     9241 gctcccgggc attgggccgc acctacccca agcccgatgc ctgaagtcgc tcccttagcc
     9301 cgattcggtc tcgcgcgccg ctccttctaa gtctgtctca tcagggacgt cggccgcgcc
     9361 ctccatgacg tcgagggtga gcaggccttc ctccgccccg gcgactactt tatcgccggg
     9421 gttcaaggag ccaccgagga ccatgccggc cagttcgttg tccacgcggc gctggataac
     9481 ccggcgcagc ggccttgcgc cgaattcggg atcgtagccc tcctcggcga tcaggtcgac
     9541 ggcggcgtcg gtgaattcca cctcgatgtc ctgcgcgtgc aggcggcgct gcgtgcgctc
     9601 caggagcatc cgcgccacgt cggctatctg ctcgcgggtg agcgccctga acacgatgat
     9661 ctcgtctatg cggttgacga actcgggcct gagctggctc ctcacgatct tcatcacgtc
     9721 ctccttgaga tcctcgaagg actctttcct cctcgagtgg gcctgtatcc tctgtgcgcc
     9781 catgttcgag gtcatgatga tgacggtctg tttgaagtcc accgtgcggc cctgggagtc
     9841 ggtaagcctg ccgtcgtcca gtatctgcag caggatgttg aacacgtcgg gatgggcctt
     9901 ctcgatctcg tccaggagca gcacgctgta cggtcggcgc cttacgctct cggtgagctg
     9961 gccggcctcc tcgtagccga cgtagccggg aggcgccccg accaggcgcg agaccgtatg
    10021 ccgttcctgg aactcgctca tgtctatgcg taccatcgcg gcctcgtcac cgaacaaggc
    10081 ctcggccagc gtgcgagcga gctcggtctt gccgactccg gtgggaccga ggaacaggaa
    10141 gctgcctatg gggcggttgg gatccccgag accggccctg gcccgcctga tggcctcggc
    10201 gatggccgtg acggcctcgt cctggccgat cacccggcca tgcagctgct cctcgaggcg
    10261 catgagccgg tcgcgctcct cctgggtgag ctggcttacc gggatgccgg tggcgcggct
    10321 taccacctcg gcgatgtcct cggcggtcac ctcggccgtg ggacgccggc cctggccggc
    10381 ctccaacctg ttctcggact cctgtatccg gttctttaag tcctgggctt tctcgaagtc
    10441 ttcggccgac accgcctggt ccttctcgcg cttgaggcgc ccgatctcgt cttcgagctc
    10501 cctcgtgtca actggcttgg tcttggagcg gagcctgacc cgggcggccg cctggtcgat
    10561 gaggtctatg gctttgtccg gcaggaagcg tccggtgatg tagcggtcgg agagctcggc
    10621 cgccgcgact atggcctcct ccgttatctt taccctgtgg tgagcctcgt aacggtcttt
    10681 gaggccgcgc aggatctcca ccgtgtcgtc gacggagggc tcgccgacca gcacgggctg
    10741 gaagcgccgc tcgagggcgg cgtccttttc gatgttcttg cgatactcgt ctatagtggt
    10801 ggccccgacc acacgaagct cgccgcgggc gagcgcgggt ttgagcatgt tagaggcatc
    10861 catcgaaccc tcggccgccc cggccccgac gaccgtgtgc agctcgtcga taaacacgat
    10921 ggcgtcctcg gagtgctccc tgatctcttc tatgaccttc ttgagtcgct cctcgaactg
    10981 cccgcggtac tgggtccccg cgaccagacc ggcgaggtcg agctgcagga cccgcttgcc
    11041 tgccagggtc tctggtacct catcgttcac tactctttgt gcgatgccct cgacgatcgc
    11101 ggtcttgccg acgccgggat ctccgataag gacggggttg ttcttggtac ggcggctcaa
    11161 gatctcgatg gtggtctcga cctcgtctgc cctgccgatc accgggtcga gcttgccctc
    11221 cctggcggcc tccgtaaggt cgcggctgta ttcgtccagg gtcggcgtcg agctcgctgg
    11281 ttcccgaacc tcgccgccgg cttcgacgcc gcgcaccacg gccccgcgca gcctggtgtg
    11341 cgagagcccg aaacgcgaga ggagccggcc ggcatccgac tcgtcgtcgg ccgcgagggc
    11401 gagcagaacg tgctcgggcc cgatgtacga ggcgcccagg gccctggact cgtcgtaggc
    11461 cgcgagcaac gccctcttgg catcgggggc cagcgtgggg gagacgtccg tgcgcccacc
    11521 cttctgggct tcttcctcca gctgcgcccg cagggcgtcg ggatccgcgc cggccccttc
    11581 gagtacccgc cgcaccaccg tgtcctcgag ggcggcgtag agcaggtgct cgcccgtcag
    11641 atcgaggctg ccccactcga gggcctgctg ggccgcgcgc tgcaacagct ccgtggtggc
    11701 cgcggagaag aactgggtca cgtccacctg ctccgcccgc ctccggaggg ccgttccccc
    11761 gcctggaagc gaggtgcgac cctcgtccaa gaacctgtcg aagaagtcgt cgaagaggct
    11821 accgccgccc agggaagatg cccctagcgg cgggcggcca cccctggcct ccgccgcgtg
    11881 gagttcgcag aggtaccgga ccgtctggcc ctcgcccggt attctccgcc tgaccgcgac
    11941 ggcggccggc cttaccccgc aaacgtcgca catcctcgtg gccatggttc tttccctcct
    12001 tctctacctc caaccgcccc cattatcgcg gatgagacgg gcgtccgtcg tttggggagg
    12061 cggcggccgg ccacgctggc tacaatgaaa atccgcaacc cggtctttag gagaggcgta
    12121 gaggggaccc attcatgaca gaatcatcca tccgcgagcc gcatcccgca ccgtggaccg
    12181 gggcctggga ccagatcgac gacgacttcg aagaccatct ggaggagata cgtaccttcc
    12241 tgcgatgccc caccgtctcg gcttcggacg acgacatgct cgccggcgcc gagtacgtgg
    12301 cggccctgat cgaggacgcc ggcggaagcg ccgagatatt acccacggcc ggacaccccg
    12361 cggtcctcgg ccgcatcgaa ggcgacggcc cgaggctcct gcgctacggg atgtacgacg
    12421 tgcagccggc cgatgaacct ggatggtcct cgcccccttt tggtgccttt acggccgatg
    12481 tgccgggagc tgggccgagc atcgtggcgc ggggcgccgc caattcgaag ggttcgctcg
    12541 cctccttctt gctggcgctc gcctcggccc gccgggtctc ggacctgccc gccgaggtcg
    12601 tctttctcgt ggacggcgag gaggagctgg gaagcccgag cctgccgggg gtgatgtcct
    12661 ccaggcgcac cgacctcgcg gccgaagccg ccttcgacat ggacctgacc gccgacaggc
    12721 gggacgtcgc tgaggtctac ctcgggtgca agggtatctt gtccttcgtc ctgggctgtt
    12781 cgggcggcga gtggggcggt cccgtgggga gggcgctcca ctctagcgag ggggtggtga
    12841 tctcatcccc cgcctggtcc ctggcgcgcg ccctttccgc ccttcgggac gccgccgaac
    12901 gagacgccct cgacggcctc ggggccgcgt cggttcctcc cgaagacgag gggctgctcg
    12961 acgccctggc gcgagagttc gacccggccg cgcatctgga agaatcggac gcgctggcgt
    13021 accggggacc ggacgacgcg cgttcgatct tgaaggcgct catgtacgag ccggtactca
    13081 acctgaacgg gttctcctca ggcagcgtta cgggaaagac catcgtgccc cacgaggcca
    13141 gggccgcact cgacatcagg attccctacg gaacggacct cgagtcagtg gtgacaggcg
    13201 tcgagcgaat cgtcgcgcag tcagctcccg aggtggtggt ggagggggtg caactctgtc
    13261 cgccgtccag gacgaccgcg gattccccgg tcgcgcgcgc catggtcgag tcccaactcg
    13321 acgtgggacc gccgccgcag gtgtggccat cggccccctg gtgggcgcct ttctacctct
    13381 acgaacaggt cctcgaggtc accttcgcgt ccggcggcgc ggggcatgcc ggccgcgcgc
    13441 acgccgccga cgagtacgcg accatcgccg gcctgaggac gcacatgcgc cagtctatag
    13501 ccttcctgca ccgctttgcc agggctgccg aggcggaccg gtgagtccgc ggggggagtc
    13561 cgggccaggc aagaccggga ggcatactgt gtggactgaa cccctggagt ttgggaagct
    13621 gacttggccc gaggtggcgc gggcggccgg tgaaaggcgc gtcccgatag tgcccatcgg
    13681 gacgctggag gaccacgggt atcatctgcc catcgacacg gacgttacgc tggtcgaggc
    13741 ggtgtgccgg ggcgccgcag agcgtctgcc agaggagacg gtgctcttgc cgcccatagt
    13801 gcacggttat tcgccgcacc acatggattt ccctggcacg gtctcgatcc aatgggacac
    13861 cttctgccgc tactgcacag acgttgccct ctcattggtg cgccatggtt tccagcgggt
    13921 actgatggtc aatggccacg ggtccaacca gaacctggtg gagaccgcgg cccgtctggc
    13981 gatggtggag gagccggaat cgctcgtcgc ggccagcttc tacctcgt
//
LOCUS       DATMVF010000302         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_39388, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000302 DATMVF010000000
VERSION     DATMVF010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39388"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VN178_15855"
     CDS             <1..367
                     /locus_tag="VN178_15855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWS82493.1"
                     /translation="ILAGLQSIPGELYEAARVDGANATQRFFAITMPQLQPVLYVVGL
                     IGTLWAVNIFDVIWLLTQGGPSGATQTLPVLIYDRAFNGFALGEASAISVLLCIFLLI
                     FSVLYIRFVPAGESDTEVF"
     gene            369..1193
                     /locus_tag="VN178_15860"
     CDS             369..1193
                     /locus_tag="VN178_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWS82494.1"
                     /translation="MGYAVRQGFRYVLVAVVLGIVVVPFLWIFLASFRPNSDLITRTP
                     SFDLGNLTLANYQNLQQAAQYFTYLTNSAIVAVTSTIVSITLAMLAAYSVYRTRYPGR
                     RPLYLALIITYVFPGVVLLIPLYQLMGMVGLIDSLASLVIVNVTFTAPFSVWLMRGFF
                     GAIPAGIEEAAAIDGAGRMRILFFIVLPLTAPGIAAAAIFSFVWSWTEYMFASAFIVN
                     EGKKTLPVGLGAFIQQYNIDWGILTAAAVATAVPVIVIFAFIGRYFVEGLTAGAEK"
     gene            1220..2437
                     /gene="thrC"
                     /locus_tag="VN178_15865"
     CDS             1220..2437
                     /gene="thrC"
                     /locus_tag="VN178_15865"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWS82495.1"
                     /translation="MTTVEAILACHSCSWRGEISNLYSCPVCGSSLLVAYAGTYELPN
                     GMVDEGIWRYRRFLPVSANSEPVTLGEGATPTLQARRLDPSGGLLLKNETVNPTGSFK
                     DRPVAVAATVARELGLSGLVCASTGNTGVAVAAYAARAGLPAACVVPEATPAAKTAQI
                     GAVGARIVRVRGNYSDAYALARAAESYGWANLTSTYINPYMLEGDKTVAYEIFEQLGE
                     RIPDWVIVPVGAGPLLAAIHKGFEELGVSGPRMVAAQAASCSPVVSAFESGAKEVSEW
                     EHSVETAASSIADPLRGYPDDGTRTLSVVRQSGGTAIAVSEGETREATIDLARSEGLL
                     VEPGAAVAVAAYRKLAAQAVISRGERAVVVLTGHGLKDPDALRSAAGSEAETGVVEAG
                     DVEALGSALEVYK"
     gene            2434..3495
                     /locus_tag="VN178_15870"
     CDS             2434..3495
                     /locus_tag="VN178_15870"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWS82496.1"
                     /translation="MKEVKVGVVGLGRFGRLHTRSLSELPGCRVSALCEIDRPTLEGC
                     GKEFGVEGLYTDLEAMLDSEDLDAVDIVTDESSHGRQVMSCLEHGRAVFVEKPLATDS
                     IEAEAVARMSRDTGLPVVVGNISRFDARYATLHRELEAEKFGRVALIQAKRSFSRTWF
                     AGFGSRVHPVFESMIHDLDLALWYLPSPVRRVYAQTGSTGDGDGVPNTLVATLTAEDG
                     ALAVLQSAWLVPDAAPITLAGLPAGPLDLWGTIDAQLEVVGTSQVGKVDLMADGLHLW
                     NDSAVRAPDTGLWPEVHGRVSGALREELSHFLDCVRARRPSSLAPPEVGAAAVRLAEA
                     IVRSSREGRVVLLPEEGAL"
     gene            3492..4244
                     /locus_tag="VN178_15875"
     CDS             3492..4244
                     /locus_tag="VN178_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWS82497.1"
                     /translation="MSGVRLTERVSVVGGGATGLGISHTLDPNVYLLSGGEELALVDA
                     GAGPGETRILENVRSLGYDPERIGHIFLTHAHADHAGGAASLAEQLGAQVYLSELERE
                     ALENADEEALGLSIARRNGFYPDDYRLDPCKVDVPLGGNEKLRCGDLDLAVTPTPGHS
                     AGSVCFLVKVEEGLALFAGDTVFAGGRISLIVAPGSDLLVLQESVTRLGGLGVASLLP
                     GHGIFPLEGAQEHIDQAIEAFGTMLPPRSILQ"
BASE COUNT          727 a         1322 c         1462 g          830 t
ORIGIN      
        1 catactggcg ggtctacaga gcatccccgg cgagttgtac gaggcggcca gggtcgacgg
       61 ggctaacgcc acccagcgct tcttcgcaat caccatgccc cagctgcagc cggtgctcta
      121 cgtggtgggc ctcataggta cgctatgggc ggtcaacata ttcgacgtca tttggctgct
      181 gacccaggga ggaccttctg gcgccacgca gacgctgccg gtcttgatct acgacagggc
      241 cttcaacggc tttgccctgg gtgaggccag cgcgatctcc gtcctgctgt gcatattcct
      301 cctgatcttc tcggttctct atatcaggtt cgtgcctgct ggcgagtcag acacggaggt
      361 gttctagcat gggttatgcc gttcggcagg gctttcggta cgttctggta gcggtggtcc
      421 tgggcatcgt ggtcgtgccc ttcctgtgga tctttctcgc gtcgttccgt ccgaacagcg
      481 acctgatcac gcgaaccccg agcttcgacc tcggcaacct caccctggcc aactaccaga
      541 acctgcagca ggcggctcag tactttacgt accttacgaa cagcgcgata gtcgcggtca
      601 ccagcacgat cgtgtccata actctggcga tgctcgccgc ctacagcgtc taccgcacca
      661 gataccctgg tcgtcggccc ttgtatctgg cgctaatcat tacctacgtc tttccgggcg
      721 tggtgctgct catcccgctc taccagctca tgggcatggt ggggctcatc gatagcctcg
      781 cgagcctggt aatcgtcaac gtcaccttca ccgcaccctt cagcgtctgg ctcatgcgcg
      841 gcttcttcgg ggcgataccg gcgggcatcg aagaggcggc ggctatcgac ggcgccggga
      901 ggatgcgcat cctcttcttc atagtcttgc cactcaccgc gccgggaatc gcggcggccg
      961 ctatcttttc cttcgtgtgg tcctggacgg aatacatgtt cgcctcggca ttcattgtca
     1021 acgaagggaa gaagaccttg ccagtgggtt taggggcttt tattcagcag tacaacatcg
     1081 attggggcat cctcaccgcg gcagccgtgg ccactgccgt accagtcatc gtgatcttcg
     1141 ccttcatcgg tcggtacttc gtcgagggtc ttaccgctgg cgcagagaag tagcggtggg
     1201 tgcggagaga aggagtatcg tgaccaccgt agaggcgatc ctggcctgcc acagctgctc
     1261 ctggcgtggg gagatctcca acctctactc ttgcccggtg tgcggctctt ccctgctcgt
     1321 cgcctacgcg ggaacctacg agctgccgaa cggcatggtc gacgagggga tatggcgcta
     1381 ccggcgcttc ctcccggtat ccgcgaactc ggaacccgta acgctcggcg agggagccac
     1441 tcctacgctc caggctcgac ggttggatcc ctctggtggg ctgctgctga agaacgagac
     1501 cgtgaacccg acgggttcct tcaaggatcg tccggtggct gtcgccgcga ccgttgctcg
     1561 agagctgggt ctctccggct tggtctgcgc ctccacgggc aacacgggcg tagcggtggc
     1621 agcctacgca gcccgggccg ggctcccggc ggcctgcgtg gtgcctgaag ccaccccagc
     1681 ggccaagacg gcgcagatcg gggcagtagg cgcgcgaatc gtccgcgtgc ggggcaacta
     1741 cagcgacgcg tacgcactgg cacgggcagc ggagagctac ggctgggcca acctgacgtc
     1801 tacttacatc aacccctaca tgctcgaagg cgacaagacc gttgcgtacg agatctttga
     1861 gcaactagga gaacggatcc cagattgggt gatagtgcct gtcggagctg gcccgctcct
     1921 cgcggccatc cacaagggat tcgaggagtt aggagtgtcc ggcccgcgga tggtggcggc
     1981 ccaggcggcg tcatgctcgc ccgtggtgag tgccttcgag tccggggcga aagaggtcag
     2041 cgagtgggag cattccgtcg agaccgcggc gtcttcgatc gcggacccgt tgagaggtta
     2101 tccggatgac ggaacgcgga cgctctcagt ggtgcgccaa agtggtggaa cggccatcgc
     2161 cgtttccgag ggggaaaccc gcgaggcaac gatcgatctc gccaggtcgg agggcctgct
     2221 cgtggagccg ggagccgccg tagcggtagc cgcctaccga aagcttgccg ctcaggcggt
     2281 cataagcagg ggggagcgag cggtggtcgt cctcacaggg cacggtttga aggatcctga
     2341 cgcgctgcgg tccgcagccg ggagcgaagc cgaaacgggg gtcgttgagg ccggcgacgt
     2401 cgaagcgctc ggatcggcgc tggaggtata caaatgaagg aggtcaaggt tggcgtggtc
     2461 ggcctgggca ggttcggccg gcttcataca cgaagcctct cggagctgcc ggggtgccgg
     2521 gtgagtgccc tgtgcgaaat cgacaggcct accctcgagg gttgcggcaa ggagttcggc
     2581 gtcgagggcc tctatacaga cctagaggcc atgctggact cggaggattt ggacgccgtg
     2641 gatatcgtga ccgacgagtc ttcgcacggc aggcaggtga tgtcgtgcct agagcacgga
     2701 agggcggtgt tcgtggagaa gccgctcgcc acggacagca ttgaagccga agcggtggca
     2761 cgaatgtccc gtgataccgg cctacccgtg gtggttggaa acatctcgcg cttcgacgcc
     2821 cgctacgcca ccctgcaccg cgagctcgaa gccgagaaat tcgggcgcgt ggcacttatc
     2881 caggccaagc gcagcttctc tcgaacctgg ttcgccggct tcggaagccg ggtgcatccc
     2941 gttttcgaat ctatgattca tgatctcgac ctcgctctgt ggtatctgcc ctcccccgta
     3001 cgaagggtct acgcgcagac aggctcgact ggcgacgggg acggcgtgcc gaacacgctg
     3061 gtcgccaccc tcaccgcgga ggatggcgcg ctcgccgtcc tccagagtgc ttggctcgtt
     3121 ccggatgcgg cgcccataac ccttgccggt ctacccgcgg gtccactgga cctgtggggt
     3181 accatagacg cgcagctaga ggtggtcggc acctcgcagg tggggaaggt ggacctgatg
     3241 gctgacgggt tgcacctctg gaacgactcg gcggttcgag cgccggacac tgggctctgg
     3301 cccgaggtgc acggacgcgt ctcaggggcg ctacgcgagg aactctccca tttcctcgac
     3361 tgcgtgcgcg caaggaggcc gtcctcgctg gcgccgcccg aggtcggggc cgctgcggtc
     3421 cgcctcgctg aggccatagt ccgctcgtcc cgggagggta gggttgtttt gttgccggag
     3481 gagggagcgc tatgagcggg gtgcgcttga cggagcgggt ctcggtcgtc ggcggcggcg
     3541 ctaccgggct aggcatctcg cacacccttg atcccaacgt gtacctcctc tccggaggcg
     3601 aagagctggc tctcgtggac gcaggggccg gtccgggcga gaccaggata ctggagaacg
     3661 tgcggtcact ggggtacgac cccgagcgga tcgggcacat cttccttacg cacgcccacg
     3721 ccgaccacgc cggaggcgca gcctctcttg ccgagcaact gggggcgcag gtctatcttt
     3781 cggagctgga gcgagaggct ctggagaacg ccgacgagga ggccctcggg ctctcgatcg
     3841 cccgacggaa cggtttctat cccgatgact accggctcga tccctgcaag gtggacgtgc
     3901 cgctcggggg aaacgagaag ctaaggtgcg gggatctgga tctggcggtg acgccgacgc
     3961 cgggtcacag cgccggctcc gtctgctttc tcgtgaaggt cgaggagggg ttagccttgt
     4021 ttgccggaga cacggtcttc gctgggggac ggatcagcct gatcgtggct cccggttcgg
     4081 acctgctcgt cctgcaggag tcggtaaccc gtctcggagg attgggtgtg gcgagcctgc
     4141 ttcctggtca cggtatcttc ccgctggagg gagctcagga gcacatagat caggccatag
     4201 aagccttcgg gaccatgctg ccgccgaggt cgatactgca atgagcgagc tgcgggatgc
     4261 tttgcgggat aatctggcgc aggcacgtga cgatctggaa ccggagctga tcgaggtccg
     4321 gcgcgatttc cacggggaac c
//
LOCUS       DATMVF010000303         9186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_39406, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000303 DATMVF010000000
VERSION     DATMVF010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39406"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..384
                     /locus_tag="VN178_15880"
     CDS             166..384
                     /locus_tag="VN178_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82498.1"
                     /translation="MADTKSMLRYDVKSVDSSEVETTGEKDLTFAVFSLAHAACGDNV
                     LEVRIGERMLLEWCMGCAVMEVFGPYER"
     gene            685..1785
                     /locus_tag="VN178_15885"
     CDS             685..1785
                     /locus_tag="VN178_15885"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HWS82499.1"
                     /translation="MRRTAVVVARAIEERFDAYRFGLKRRLGLLDPFEILPYRGYGTA
                     RELFLKGRVLEESGITHAGRDDAVWKNVLNMARRFASDEVAGARVKASFEDLHVETIA
                     DVEGFFEVRFQLAQPLGGRHGWHQVGLELLSPPSPGGGRVRAKGHVLVPHGARFGVIS
                     DLDDTVVRSSATSVLKMAWIVVRNNAHTRLPFEGVAAFYRALQLEPDSRSSNPIFYVS
                     SSPWNIYDMLVDFLNVHGVPHGPLFLKDWSLNVLGKHRDYKLGVIRHLLSTYKGMPFV
                     LIGDSGEEDPEIYLQAVREHPGRVIAVYIRDVTSGKRDAEIRAMAGEARKLGTEMVAV
                     PDTTAAAEHAASIGLVAPSAIDTLRAGSTASY"
     gene            complement(1796..2296)
                     /locus_tag="VN178_15890"
     CDS             complement(1796..2296)
                     /locus_tag="VN178_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWS82500.1"
                     /translation="MSIEKSEILIGPDEGAYLPVLDIVHKVTAEASGGSLKIEEWGLP
                     AGQMIPPHTHAREDECSFVLEGEMKCYVDGEVVLAQKGSYVIKPRGVPHAFYNSGAKT
                     VRVMEILTPGDSFEGYFDAYEEIVSRQMSDEEHRKKRAELGKRYGITWHDENIAEVEA
                     LFGIGS"
     gene            complement(2301..2459)
                     /locus_tag="VN178_15895"
     CDS             complement(2301..2459)
                     /locus_tag="VN178_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82501.1"
                     /translation="MYGLSEYLIALQEAEKVRKESKARKTPREALPDRENDSSASGGY
                     TDQRTRRR"
     gene            complement(2507..3490)
                     /locus_tag="VN178_15900"
     CDS             complement(2507..3490)
                     /locus_tag="VN178_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82502.1"
                     /translation="MKATTRKVMNIGRATTIAAVMISAGLFVALAETAREAEATFPGE
                     NGRIAFASNRTTGEGVNNPEGDFEIFTMNQDGTGLTQLTENAAFDSNPEWSPDGGRIA
                     FESDRDLFSDIFVMDSDGSGQTNVTNNRAFDRSATFAPDGERIIFDSNLSAGVDNPSG
                     DTEVFSINLDGTDLAQLTNNTARDFHPVFEPDGRKIAFVSDRNFAPGIYTMSADGSKQ
                     KKSNRGSGVAFASPGWSPDGSRITFASDQEGGHDVYVMRANGAGQQRLTVNGLPTDSG
                     PVFSPDGGQIALQTNRDGNFEIYAMGPNGEHQTNLTNHPAADLSPDWQPLR"
     gene            complement(3669..4307)
                     /locus_tag="VN178_15905"
     CDS             complement(3669..4307)
                     /locus_tag="VN178_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82503.1"
                     /translation="MMKITKTILARIWWLGRGTATMMGVAIMLAFTVGLASTALAGTG
                     IGARFDLGKTNTVNAVSKLVGNVAGASLLIDNNSTNASATALGLRVEPGKAPMRVNPE
                     AGTATNLSADELDGKSADEIGVNGLERVVTSSAFNSDSFKRVTARCPVGKVVVGTGYQ
                     ISDDQQDFQEAANFGVIAVIPGIDLVEVIAAEQAPTSANWIVDAYAVCATAP"
     gene            complement(4564..5025)
                     /locus_tag="VN178_15910"
     CDS             complement(4564..5025)
                     /locus_tag="VN178_15910"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HWS82504.1"
                     /translation="MKDSDFADARILPLLTEAKHRRDWYVAREYGGEDGSYSLKVFVW
                     PAGTGTRIHDHSSWGAYACASGTILEERYDRLDDGTIAEHARLTKAWQLRWSPEDGAS
                     TVLPDDGGIHRVGNPGEGVAVSVHLYGPRIGEVDGRDYDPSRDYVCDRRED"
     gene            5356..7731
                     /locus_tag="VN178_15915"
     CDS             5356..7731
                     /locus_tag="VN178_15915"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS82505.1"
                     /translation="MGGVQTGTELQFGDLLRRHRASANLTQEDLADKTGLTPQAIGLL
                     ERGKRRRPHGYTVQKLAEALRLEGSDLAEFEAAARRPPTRRTTAELPRRALPVPPTPL
                     IGREHEVEDVAVLLRRHDVRLLTLTGPGRVGKTRLALEVAGRAEPDFADGVAFVALAP
                     LQAPDLVPSVLAEALGVKDVTGGSLMEALSGHLQEMEMLLLIDNLEHLLPAAPMIADL
                     VRECPRLTVLATSRAPLRLMGERQLSVPPLFFGEKSSPAVRLFCERAVAVAPAFELTA
                     ANASTVEEICRRLDGLPLAIELAAARIKLFSPEALMDRLDRRLRVLSGGARDLPERQR
                     TLRDTVAWSHDLLDEDERALFGRMSVFAGGFSLEAAEAVCGTPMPEGEDVLETLASLV
                     DNSLVVSRSGVSAGQEDGESRFAMLETIKEYAAERLRSSGEDDEVRRAHAHYFLALAE
                     DAQPENSPHMFEEWLVVLERDHDNFRVALRWAIRHQETDIGTRLALLLWRFWSEHYHV
                     GEGRRWLEAVLPLGGPEGGVAEPTLPARRWAFLHLVAGILASGQGDYDRAVELCEQSL
                     SLYRRMGYRKGTSGPLRELGAVAYHRGDYDEAVCLSEQALALTHEFGSTFGHGLAVCT
                     LADALRAQGDVERARALLEESLTSLRRKTYLLRVANALANTLARLGSIECELGREARA
                     FELFGESLWLGRRFGFIHHLVIPLEGMARVAAVRGRPEWAARLLGTSAALRDEMGATL
                     TLVARTDHDYALDAARAKLGEDAFAAEWERGRAMPVEVAISAVLDDEELSP"
     gene            7841..8335
                     /locus_tag="VN178_15920"
     CDS             7841..8335
                     /locus_tag="VN178_15920"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HWS82506.1"
                     /translation="MKEHELEETGERTMEDPQERSARELIELLSELRVILPGVQFLFA
                     FLLTVPFSPRFPQLDGLETGIFFVTLLCTAIATALLMAPSAQHRVLWREGVREQRLEL
                     GNTLTIAGLAFLVPAMVGAVFVVSAFIFGLGIGVAVTTLLVLFFALLWFALPLRYRGA
                     DAEE"
     gene            complement(8340..>9186)
                     /locus_tag="VN178_15925"
     CDS             complement(8340..>9186)
                     /locus_tag="VN178_15925"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWS82507.1"
                     /translation="AREVGVPLDIDDFDPVSNRTPIIADLKPGGRYTAVDVDRAGGSR
                     LLGKRLLDAGLIDGNQLTTTGRTLAEEVEDAEETPGQDVIVSVEEPLRPSGGLVILKG
                     NLAPQGCVVKVAGYTRLEHTGPARVFDSEEDAMKAVQAGEITDNDVVVIRYEGPKGGP
                     GMREMLGVTAALVGQGFGETVALLTDGRFSGATRGLMAGHVAPEAAHRGPIAALEDGD
                     IVTFDIENRTLSVDLPTEELESRLADWEEPEPRFATGVMAKYAALVSSASEGAVTDPK
                     LGPAA"
BASE COUNT         1453 a         2894 c         3016 g         1823 t
ORIGIN      
        1 cccggagggc ctacaagcac ctgggcgttc ccgttcccaa cggcggctag gatcgtctgc
       61 ttgtagggcg tttcgcgaaa cgccttacga cgaactacgg caacaggccg cagcactcga
      121 tgttgcgctt aggagtccga atacccgtta aggtcgaatt gcagagtggc cgatactaag
      181 agcatgctgc gctacgacgt aaaatcggtc gactcttcgg aggttgagac cacgggcgaa
      241 aaagacctca ctttcgccgt cttcagcctg gctcatgcgg cttgcgggga caatgtgctc
      301 gaggtgcgta tcggcgagcg catgctgctc gagtggtgca tgggatgcgc cgtcatggag
      361 gtcttcgggc cgtacgagag atgatctgaa agcgtgatgc gtaagagtcc cttctcgctc
      421 ttgcaggcat caggtttcag ggattcgctg caacagagca gcgcgagcgg ctttgacccc
      481 tgcttctcaa accaacagtc attcccgctt gctcgggaat ccaatgcgcc ggacgctcgc
      541 cgccccaatc cggatgcgac cggattacgc atcaagtgcc tttcgctgct tgaatagtgt
      601 ccgaaacctt gtccttgaac cgcccgtcgt gttagtttcg gttccacgcg atgtgtggag
      661 tcggaaggac tctggttgat cgacgtgagg cggactgcgg ttgtggttgc gcgggccatc
      721 gaggagcgct tcgatgccta caggttcggg ctgaagcggc gcttgggact tctcgacccg
      781 ttcgagatcc taccttaccg gggatacggt accgctcggg agctgttcct gaagggacgc
      841 gtcctcgagg aatctggcat tacccacgcg ggtcgggacg atgcggtgtg gaagaacgtc
      901 ctcaatatgg cgcggcgctt cgcgagcgat gaagtggccg gcgcgcgggt gaaggcttct
      961 ttcgaggatt tgcacgtcga gaccatcgcc gacgtggagg gtttcttcga ggtgcgcttc
     1021 cagcttgcgc agccgctcgg cggtcggcat ggatggcacc aggtcgggtt ggagctcctc
     1081 tcgccaccgt cgccgggtgg aggaagggtg agagcgaagg gacatgtgct cgtgccgcac
     1141 ggagcccgtt tcggtgtgat cagcgacctc gacgatacgg tagtgcgttc cagcgcgacc
     1201 agcgtgctga agatggcctg gatagtggtg cgcaacaacg cccacacgcg gctgcctttc
     1261 gagggcgtcg ccgcgtttta cagggctctg caactggagc cggacagcag atcttccaac
     1321 ccgatcttct acgtgtcgag cagtccctgg aatatctacg atatgctcgt ggatttcttg
     1381 aacgtgcacg gcgttcccca cgggccgctc tttctcaagg actggagcct gaacgttctc
     1441 gggaagcaca gagactacaa gctaggcgtg atccggcacc tcctcagcac atataagggc
     1501 atgcctttcg tcctgatcgg ggactctggg gaggaggacc cggagatcta cctgcaggcc
     1561 gtacgggagc acccgggacg cgtcatagcc gtctacatca gggacgttac ttcgggcaag
     1621 cgtgacgccg agatccgcgc aatggccggc gaggcgcgca agctggggac ggagatggtt
     1681 gccgtgcctg ataccaccgc ggctgccgag cacgcggcct ccatcggtct cgtagcgccg
     1741 agcgcgatag atacgctccg ggcaggaagt actgccagct actagcgcac tcttatcaag
     1801 aaccgatacc aaacagggct tctacctcgg caatgttctc atcgtgccag gttatcccgt
     1861 agcgcttgcc tagttcggcc ctctttttgc gatgctcctc gtcgctcatc tgccgggaga
     1921 cgatctcctc gtaggcgtca aagtagccct cgaacgagtc gcccggcgta aggatctcca
     1981 taacccgtac ggtctttgcg ccagagttgt agaaggcgtg gggtacgccc cgcggtttga
     2041 tgacgtagga ccctttctgg gcgagcacaa cttcgccgtc tacgtagcac ttcatctcgc
     2101 cttctaacac gaatgagcac tcgtcttcgc gggcgtgggt gtggggcggg atcatctggc
     2161 cggcaggaag gccccactcc tcgatcttaa gcgaacctcc ggaagcctct gccgtaacct
     2221 tgtgcactat gtccagcacg ggaaggtagg cgccttcgtc cgggccgata aggatctcgc
     2281 tcttttctat ggacacccgc ttacctcctt cgagttcgtt ggtcagtgta cccgcctgac
     2341 gcggaagagt cgttctccct gtccgggagg gcctcccgag gagtctttcg cgccttgctc
     2401 tccttgcgca ccttctcggc ctcttgcagg gcgatcaggt actcgcttag cccgtacact
     2461 ttagcctcct gtctatcggc tgctacgagc acctcttcta ataccgctac cgcagcggct
     2521 gccagtcggg ggagagatct gcggccgggt ggttggtgag gttggtctgg tgctcgccgt
     2581 tggggcccat ggcgtagatc tcgaagttgc cgtcccggtt agtttgcaaa gcgatctgcc
     2641 caccatccgg tgagaagacg ggtccgctgt cggtcggtag gccgttgacg gtgagccgct
     2701 gctgtccggc gccgttggcg cgcatcacgt agacgtcgtg accaccttct tgatcgctgg
     2761 cgaacgttat cctcgagccg tccggcgacc agccggggga tgcgaaggct acccccgagc
     2821 cgcggttgct cttcttctgc ttcgagccgt cggcgctcat ggtgtagata cctggcgcga
     2881 agttccggtc gctcacgaag gctatctttc tgccgtccgg ctcgaagacc ggatggaagt
     2941 ccctggccgt gttgttcgtg agctgagcca ggtccgtgcc gtcgaggttt atcgagaaga
     3001 cctcggtgtc cccgctcggg ttatccacac ccgcgctcaa gttgctgtcg aagattatcc
     3061 tctcgccatc cggcgcgaag gtggcggacc tgtcgaatgc ccggttgttg gtgacgttgg
     3121 tctgtcccga gccgtccgag tccatcacga agatgtcgga gaagagatcc cggtcgctct
     3181 cgaaggctat ccgcccaccg tccggggacc actcggggtt ggagtcgaag gcggcgttct
     3241 ccgtgagctg ggtcagccct gtgccgtcct ggttcatcgt gaagatctcg aagtcacctt
     3301 cggggttgtt gactccttct ccggtggtgc ggttggaggc gaaggcgatc ctgccgttct
     3361 cgccggggaa ggtcgcttcg gcctccctcg ccgtttccgc cagcgccacg aacaggccgg
     3421 ccgagatcat cacggcggct atcgtcgtcg ccctgccgat attcatgact ttcctggtcg
     3481 ttgccttcat ctttatccgt ttcctttctg ttcgggggcg gaccgtccag ccccgtgcgt
     3541 ttctctccct acggcctcag tggcgggacg gtccgcgcag accacgttcg acggcatgag
     3601 tcgaatgcgt ggttgaagaa agtgagccga tagataccgt cgctgatgcg ctcgagagct
     3661 ccctatcact acggtgcggt ggcgcagacc gcgtaggcgt ccacgatcca gttagcgctg
     3721 gtcggcgcct gctcagccgc tatcacctcg acgagatcaa taccagggat tactgcgatg
     3781 accccgaagt tggcggcctc ttgaaagtct tgctggtcat cggagatctg gtagccggtc
     3841 cccacgacca ctttccccac cgggcaacgc gcggtcaccc tcttaaagga gtccgagttg
     3901 aaagcgctac tggtgacgac cctctccagg ccgttcaccc cgatctcgtc cgcgctcttg
     3961 ccgtcgagtt cgtcggcgct gaggttggtg gccgtgccgg cttcggggtt gaccctcatc
     4021 ggagccttgc ccggctcgac ccgcaagccc agcgccgtgg ccgaggcgtt ggtggagttg
     4081 ttgtctatca gcagggaagc tcccgctacg ttgccaacca gcttgcttac ggcgttgacc
     4141 gtgttggtct tgcccaggtc gaagcgggcg cctatgcccg tgccggccag cgctgtggag
     4201 gcgagcccca ccgtgaaggc gagcatgatc gccacgccca tcatcgtggc cgttcctctt
     4261 cccaaccacc agatcctcgc gaggatcgtc ttcgttatct tcatcatctg tacactttcc
     4321 tttctagttg ttcttcggcg cttgctttcc gtgcggatac tcttcgcgtt tgcgccgtgt
     4381 cacgctacat gcctacctcc gatcctctct cacgtccctc acgccgatca cctcgtttct
     4441 ttgtattggt tccgcgttgc gccaaccacc aaggtagcct cggatattcg gggcgaacgt
     4501 ggcagaaagc tgccattccc gaacaccccg ccggtcgtcg gttgcgtgat ggccatccct
     4561 tcgtcagtcc tctcgccggt cgcagacgta gtcgcgggaa gggtcgtagt cgcgaccgtc
     4621 cacctccccg atcctgggtc cgtacaggtg gaccgagacc gccacgccct cgccggggtt
     4681 accgacccgg tggatgcccc catcgtccgg tagcacggtc gaggccccgt cctccgggct
     4741 ccaccgcagt tgccaggcct tagtgaggcg ggcgtgctcc gcgatggtgc cgtcgtcgag
     4801 gcggtcgtag cgctcctcga ggatggtccc ggaggcacag gcgtaggcgc cccacgagga
     4861 gtggtcgtgg atcctggtgc cggttcccgc gggccagacg aaaaccttca gcgagtacga
     4921 gccgtcctcc ccgccgtatt cgcgggccac gtaccagtct cgacgatgtt tcgcctccgt
     4981 caggagcggg aggattcggg catctgcaaa atccgaatcc ttcaccaggt ctgccaggaa
     5041 gcggccggcc cgcggcaggg ccgaccccgt ggggaggctg gcgatctccc gtagttcgtt
     5101 ggtgtctggg tcagtttggt cgccatctgt ttctcctgtc cttcgtcggt aacgtccttg
     5161 gggctatccg tcccgcttcc catggtgcgt tccgcgcgcc tcttctccca gcgacgaacc
     5221 caaacaacgg cccaaacaat cgacgaacac aggccagttt gtggcctctt cttcgagaac
     5281 ctgctgcccg gttgatggct atccggtaga cttcgggctt actgtggcat cgaacgagcg
     5341 gctgaggggc tgaaggtggg aggggtacag accggcacgg aactccagtt cggtgatctg
     5401 ctgcggcgcc accgcgcctc ggccaacctc acccaggagg acctggccga caagacgggg
     5461 ctcacgcccc aggccatagg cctcctcgag cgcgggaagc gacggcggcc gcacggatac
     5521 accgtgcaga agcttgccga ggccctgcga ctggagggct ccgatctcgc cgagttcgaa
     5581 gctgcggccc ggcgcccccc cactcgccgc accactgccg agcttccccg tcgcgcactt
     5641 cccgtgcccc cgacgccgct catcgggcgc gagcacgagg tggaggacgt ggcggtcctc
     5701 ctgcgtcgcc acgacgtgcg gcttctgacc ttgaccggtc ccgggcgcgt cggcaagacc
     5761 cggctggctt tggaggtggc agggcgcgcc gaaccggatt tcgccgacgg tgtcgccttc
     5821 gtagccctgg caccgttgca ggccccggat ctcgtcccct cggtcctcgc cgaggcgctc
     5881 ggggtcaaag acgtaacggg cgggtcgctg atggaggccc ttagcgggca cctgcaagag
     5941 atggagatgc tgctgctgat cgacaacctg gagcatctcc tgccggctgc gcccatgatc
     6001 gccgacctgg taagagaatg cccgcgcctg acggtgctcg ccacgagcag ggcgccgctt
     6061 cggctcatgg gcgaacggca gttatcggtg ccgccgctat tcttcgggga aaagagctcc
     6121 ccggcggtgc ggctcttctg tgaacgcgct gtggccgtgg cgcccgcttt cgagcttacg
     6181 gcggcgaacg cctctacggt ggaggagatc tgccgccggc tggacgggct tccgctggcc
     6241 attgagctgg cggcggcgag gatcaagctg ttctcgcctg aggcgctgat ggaccggttg
     6301 gatcgccggc tgcgagtgct ctcgggcggt gctcgcgacc tgccggaacg ccagcggacg
     6361 ctgcgcgaca cggtcgcgtg gagccacgac ctcctggacg aagacgagcg ggcgctgttc
     6421 gggcgaatgt ccgtgttcgc cggcggcttc tccctggagg ctgcggaggc cgtgtgcgga
     6481 acccctatgc ctgagggaga agatgttttg gagaccctgg cgtcgctggt ggacaacagc
     6541 cttgtggtct cgagatccgg ggtctccgct ggtcaagagg acggggagtc acgcttcgcg
     6601 atgctggaga ccatcaaaga gtacgcggcg gagcggctcc gctccagcgg cgaggacgat
     6661 gaggtgcgcc gcgcgcacgc gcactacttt ttggcgctgg ccgaggacgc gcagcccgag
     6721 aattccccgc acatgttcga agagtggctg gtggtcctgg agcgggatca cgacaacttc
     6781 agggtggcgc tccgctgggc gatccggcac caggagacgg acatagggac gcggctggcc
     6841 ttactattgt ggcggttctg gtccgagcac taccacgtgg gtgagggccg caggtggttg
     6901 gaggcagtgc tgccgctggg cgggccggaa ggcggggtcg ccgagcccac cctgcccgct
     6961 cggcggtggg cgttcctaca cctggtggcc ggcatactgg caagcggaca gggggactac
     7021 gaccgcgcgg tcgagctgtg tgagcagagc ctgtccctct acaggaggat gggctacagg
     7081 aaaggcacga gcggtcccct gcgcgagctt ggggccgttg cctaccaccg gggtgactat
     7141 gacgaagctg tgtgcctgag cgagcaagcc ctcgccctta cccacgagtt cgggagcacc
     7201 ttcggccacg gccttgccgt ctgcaccctg gcagacgctc tgcgcgccca gggcgacgtc
     7261 gaaagggcca gggcgttgct cgaagagagc ctgacctcgc tgcggcgcaa gacgtaccta
     7321 ctgcgcgtgg caaacgccct cgccaatacg ctggccaggc tcggaagcat agagtgcgag
     7381 ctgggcaggg aggcacgggc cttcgagctg ttcggtgaga gcctgtggtt ggggcggcgg
     7441 ttcggcttca ttcaccacct tgtgatcccc ctcgaaggga tggcccgggt ggccgcggtg
     7501 cggggccgac cggagtgggc ggcgcggctg cttggtacct cagccgcgct tcgcgatgaa
     7561 atgggcgcga cacttacgct ggtcgcccgg acggatcacg actacgccct ggacgccgcc
     7621 cgcgccaagc taggagaaga cgcgttcgcg gcggagtggg aacggggccg tgcgatgccc
     7681 gtcgaagtgg caatctccgc cgtcctggac gacgaggaac tgagcccgta accgctcgcg
     7741 gcttgccgac ccgaagccgc agtactcttt gtttatccta ccttggcgtc cgtatagaat
     7801 gtcagctacg tccgagttac acgcgcaagg gaccgggggc atgaaagaac acgagctgga
     7861 ggaaaccggg gagcggacga tggaggaccc tcaagaacgc tcggcgcggg agctgatcga
     7921 gctactgagc gagctccggg tgatactgcc tggcgtgcag tttctctttg ccttcctact
     7981 tacggttcct ttcagcccgc gcttccccca gctggacggg ctggagaccg ggatcttttt
     8041 cgtgacgctg ctttgcaccg ccatcgcaac ggcgctcctc atggcgccct cggctcagca
     8101 ccgcgtcctc tggcgcgagg gcgtaaggga gcagcggctg gagctgggga acacgctcac
     8161 catcgccggc ctcgccttcc tcgtgcccgc gatggtcggg gccgtcttcg ttgtcagcgc
     8221 cttcatcttt ggcctgggca taggcgttgc cgtgacgacg cttctggtcc tctttttcgc
     8281 gctgctctgg ttcgcgctcc cgctccggta ccgcggtgca gatgccgaag aatgaacctc
     8341 taagccgcag gaccgagctt gggatcggtg acggcgcctt ccgaggcaga cgaaacgagc
     8401 gccgcgtact tggccatcac cccggtagcg aagcggggtt ctggttcctc ccagtccgcc
     8461 agacgggatt ctagctcttc tgtaggaagg tcgacgctca gggtcctatt ctcgatgtcg
     8521 aaggtgacga tatcgccgtc ttccagggcc gctatggggc cgcggtgggc tgcctcgggg
     8581 gcgacgtggc cggccatcag gccgcgggtg gcgccggaga agcggccgtc ggtgaggagg
     8641 gcgacggtct cgccgaaacc ctgaccgacg agggccgcgg tgacgccgag catctcgcgc
     8701 atccctggtc cgcccttcgg tccctcgtag cggatcacca ccacgtcgtt atcggtaatc
     8761 tctccggcct gcacggcttt catggcgtcc tcctcggagt cgaagacgcg ggcgggaccg
     8821 gtgtgctcca ggcgggtgta gcccgcgacc ttgaccacgc agccctgcgg cgcgaggttg
     8881 cccttgagga taacgagtcc acccgaaggc ctcagcggtt cttcgacgga gacgataacg
     8941 tcctgtcccg gcgtctcctc ggcgtcctcg acctcctcgg cgagggtgcg gcccgtggtg
     9001 gtgagctggt tcccgtctat gaggccggcg tcgagcaggc gcttgccgag cagacggctc
     9061 ccgccggcgc ggtcgacgtc gacggccgtg tagcgcccgc cgggcttgag gtcggcgatg
     9121 atcggggtgc ggttgctgac ggggtcgaag tcgtcgatgt cgaggggaac cccgacctcg
     9181 cgggct
//
LOCUS       DATMVF010000304         8028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_596802, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000304 DATMVF010000000
VERSION     DATMVF010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596802"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VN178_15930"
     CDS             complement(<1..302)
                     /locus_tag="VN178_15930"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWS82508.1"
                     /translation="MLVFDPYSGKTVSLNADRRFVAASLSKLYALLTLYRAVSQGEMS
                     LDDEITMRPSDIWAYGTGVLSKNPGKYPAGYTMTLRECAEFMIKESDNTAELMLNR"
     gene            complement(768..1322)
                     /locus_tag="VN178_15935"
     CDS             complement(768..1322)
                     /locus_tag="VN178_15935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWS82509.1"
                     /translation="MMKRPKEARRLENRIFTVPNALSLARLFMVPGVVALLLARADGL
                     AAALFVLAAVTDFLDGRLARRAGPTRLGQILDPVADRLMLSSVAVVLAVRGILPAWAV
                     AILVGRDLSTLIGSLVVGSKVRVNRVGKAATALLMGAIAIVVLRPGTVVGEIMFYAGF
                     GLSIVAGLMYLRSLRRVLGRGEVR"
     gene            complement(1430..1963)
                     /locus_tag="VN178_15940"
     CDS             complement(1430..1963)
                     /locus_tag="VN178_15940"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWS82510.1"
                     /translation="MPKLSPGDNAVPFELPGVDDNNHALADYANKEAIGVVFTCNHCP
                     YARAWEDRLIEIQADFAGRGVQLVAISANDAKKYPDDSFPRMKERSEEKGFNFPYLYD
                     ESQEVARAYGAERTPEVFLLDKGGALRYHGAVDDNYDDPAAVKSHYLIDALEAVLGGR
                     EPATKETAPVGCTIKWK"
     gene            complement(2047..2370)
                     /locus_tag="VN178_15945"
     CDS             complement(2047..2370)
                     /locus_tag="VN178_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82511.1"
                     /translation="MRVSFLYYEECPSHEQALERLSEVMAEEGIFTDVEVFEVETEEQ
                     ARELRFVGSPTIRVDGQDIDPPGEARYVLACRAYRLEDNRISPLPSREMIRRALRSAA
                     KSPPG"
     gene            2565..5333
                     /locus_tag="VN178_15950"
     CDS             2565..5333
                     /locus_tag="VN178_15950"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWS82512.1"
                     /translation="MTYTAWFDEIRKDDIALAGGKGANLGELSHAGLPVPPGFVVTTT
                     AYDTFVEANGIGDAIVGRALTTRADDPVGFEEAAEAIRALFSGGKVPEELADELRAAY
                     QELGEDGETPVAVRSSATAEDLPGMSFAGQQETYLNVRGAEALLDGVKNSWASLWTAR
                     AMAYRKRQGVDPAIVSLAVVVQRMVESEAAGVMFTANPSNGRRDQATISAAWGLGESV
                     VSGSVTPDSIVVDKGSGLVLSRETADKEVMTVYTEGGTAERPVPEALRRQPVLDDEAT
                     VALTRYGATIEHNYGFPQDIEWAFAGGEFFIVQSRPITALPDPMADPPTDWSVPIPKG
                     TYWRASIVEQMPDPLSPLFADLASGAVPRSLDKLMEELFGSSVFREGDLAFPTVNGYA
                     YYYYSLGAFWRLLAKTPTAFRLLMESGDGGAVVRWREYARPRYARTVAEWEAKPPEDL
                     QAEELLAGARKLLDAGTEYYTSVQTIIPLASISEALLTMYYDRLVRRDGDPPAQTFLL
                     GFDSMPIRAEKSLYDLATWSREHPDLALALARTPSERILDLLGTEGAPPGVDTEEWGE
                     WRSRFQSHLDRYGHAVYNLDFVNPVPADDPTPLFDTLRFYLRGEGRNPHERQRGTATR
                     REDASRAVLERLDAPRGNLFSRLLYWAQSVAPVREDALADVGLAWPVMRRMLLELGRR
                     LVEAGAVEIPADVFWLSWDELEDRAGSLDAGEAQLASLAKPVEQRKMLWRGQRRVTPP
                     QLLPRGTWLEVFQRLMPAASEEQTGDTIKGVAASGGQVTAPARVLSGPEDFGQMQPGD
                     VLVAGITTPAWTSLFAMASAVVTDVGGPLSHSSIVAREYAIPAVLGTGVATRRIRSGQ
                     SVRVDGDAGTVTLLDVPGATDAGQSLPQPVLAAQGLDAGTKMVALFALAIGAVVVAAM
                     WWRKRRRS"
     gene            5446..6072
                     /locus_tag="VN178_15955"
     CDS             5446..6072
                     /locus_tag="VN178_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWS82513.1"
                     /translation="MAEVVLFHSVLGLSPGVFSAAERLGAAGHTLHTPDFFDGEVFDD
                     LEEGMRKEEALGFQEIARRARDSVAGLPEGLVFCGFSLGAVHAEALAASRPGALGAVL
                     MAGAVPVEALEEYFGIDRWPEGVAVQVHYAADDPWVEVKEEVVPLGEAVRGAGAAFEA
                     HSYPGSGHLFFEPGVPVYDRPSSEEMWERVLVFLDRIDARAWDVEGAR"
     gene            6301..7158
                     /locus_tag="VN178_15960"
     CDS             6301..7158
                     /locus_tag="VN178_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82514.1"
                     /translation="MGGLLVQFLGLVALLGLVGWGYRRWISPRRVGIQGKGMLLLCVV
                     TLVGGLLGSTGWWIDDPRAFSWDLPPVASRMLASAGWAFGVATIAALQRPVPHRMRLV
                     MLMLAAYLVPLLLTAPLFHLDRFDPAAPITYAFFALVLTMTVAATWYLVKQPVVVSDE
                     PADSLAPRALVSNWLGLVAAVCALWGLALFVTDDGPSGLIWVWPGDLLTSRLIAVMLL
                     TIALGAVYALRYADIARVMLGVIAVYGFGVMLANLWNILANLPINLSYVSVFGIMFVT
                     SATLLLAER"
     gene            7497..>8028
                     /locus_tag="VN178_15965"
     CDS             7497..>8028
                     /locus_tag="VN178_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWS82515.1"
                     /translation="MTEYPAVRIIVAPYDSGFRGLRMGAGPDCLMEGGLPEMLRSTER
                     PSLSFVRVLPEVDPPAEVAVAFELDRLVAAQVGEALEGGEFPLVLSGNCNTAVGTISG
                     LGASDLGVVWLDAHADFNTPETTTTGFTDGMGLAVAVGHCWKEMARGVSGFSAVAERD
                     VVLAGVRTVEPAEEQRL"
BASE COUNT         1385 a         2486 c         2617 g         1540 t
ORIGIN      
        1 tagcggttga gcatcagctc tgcggtattg tcgctctctt tgatcatgaa ctctgcgcac
       61 tcgcgcaggg tcatggtgta gccggcaggg tacttgccag ggtttttcga gagcaccccg
      121 gtgccgtagg cccagatatc cgaggggcgc atggtgatct cgtcgtccag gctcatctca
      181 ccttgggaaa cggccctgta gagcgtaagc agagcgtaga gcttgctaag gcttgcagcc
      241 acgaagcgcc gatcggcgtt gagtgagacc gtcttgcccg aatagggatc gaagactaac
      301 accccatgga tccctgtatg agactgggag atgtccggga gcttggatcg cagctcctct
      361 acggcttggg ttcgtgcggg tgctggaggt tgcggtggac tcgcggaagt cgggcgatgc
      421 acgctcgcaa tcgccgtagc cctggccgat cgctcagaat cccaccgaaa atagttggct
      481 accaacaggc ataacagaag gccgaatata agcagcagcc aacctctgcg tcgtcgttgc
      541 cggcgcctct ggttcctcgc gtaccgaggc gcccgattcc tcccggcgcg cttgtaagat
      601 ctcctcggtc gtcccatggc tggtgcgcac tttagcagta tctcccagcc caagtactcg
      661 caaaatctgc atcttcagcc agctctctag ctcgtgaggg accgtagccc cgtgttggtt
      721 cgcttgtggg ccggcggttt actttcctcc gggtgcgagg atcgtcttca tcgaacctca
      781 cctctgccca ggacacgccg taaacttctc aggtacatca gacccgcgac gatggagagc
      841 ccgaaacccg cgtagaacat gatctctcct accaccgtcc ccggtcttag caccacgatc
      901 gcgatcgccc ccatcagcaa cgcagtcgcc gccttgccta cccgattgac cctgacctta
      961 ctgcccacca cgagactccc gatgagcgtc gacagatcgc gcccgacgag tatggcgacc
     1021 gcccaggcag gcaagatccc gcgcacggcc agtacgaccg ccacgctgga gagcatgagc
     1081 cggtccgcca ccgggtccag gatctgaccg agtcgggtcg gcccggctcg ccgcgcgagc
     1141 ctgccgtcca ggaagtcggt cacagccgcc agcacgaaga gtgcggccgc caggccatcc
     1201 gcccgggcga gcagcaacgc gaccacgccc ggcaccataa agagccgcgc gagggagagg
     1261 gcgttgggca ccgtgaagat gcggttctcg agtcgtctcg cttcttttgg tctcttcatc
     1321 attccgtggg ctgctcttta tcctgatccc cgcgtctcac aaagatcaca acgctcccgc
     1381 agtatagact caggtggcgc gtgaagagac aacggcacct acgcggaagt tacttccact
     1441 tgatggtgca ccctacggga gcggtctcct tggtggccgg ctcgcgaccc ccgaggaccg
     1501 cctcgagggc gtcgatcaag tagtggctct tcacggctgc cggatcgtcg taattgtcgt
     1561 ctacggcgcc gtggtagcgt aaagcgccgc ccttgtcgag gaggaagacc tcgggagtac
     1621 gctcggcccc gtaagcccgg gcgacctcct gggactcgtc gtacaggtaa gggaaattga
     1681 accccttctc ctcggagcgc tctttcatcc tggggaagct atcgtcgggg tacttcttcg
     1741 cgtcattggc gctgatggcg acgagttgga cgccgcggcc ggcgaagtcg gcttgtatct
     1801 cgatcaagcg gtcttcccag gctcgggcgt aagggcagtg gttacaggtg aagaccaccc
     1861 caatggcttc tttgttcgcg tagtcggcca gggcgtggtt gttatcgtcc actccaggca
     1921 gttcgaaggg tactgcatta tcgccagggc ttagtttggg catgtaatct tccctttctc
     1981 gagcgacttc acagaacggg taacgtccct tagagacgtt ttaccatcct tcgatccggt
     2041 cgacggtcag ccagggggac tctttgccgc ggacctcaaa gcacgcctga tcatttccct
     2101 ggaaggtagt ggggatatgc ggttatcttc gagacggtag gcgcggcatg ccagcacgta
     2161 gcgagcctcg cccggtgggt ctatatcttg cccgtcgacg cggatggtcg gggaaccgac
     2221 gaaccgtaac tcgcgcgctt gctcttcggt ctcgacctcg aagacctcca catctgtgaa
     2281 gataccttct tcggccatta cctcgcttag gcgctcgagt gcctgctcgt gagacggaca
     2341 ctcttcgtag taaaggaagc tcactcgcat gaggtccctc cgatctctgt tcgttacaca
     2401 tcccagtagc tcccgtatgg acttgggatt tacggcattc atgataaggc gcactacctc
     2461 cagtttcagc ggcgatgagc ggcgaaccgc tacgaccgcg gtatagaatc tgtgtaaggt
     2521 gtgaacgacg gctggaagag gcgggcgcgg gcgagatgca ggacatgacc tacactgcat
     2581 ggttcgatga gatacgcaaa gacgacatcg cgctggcagg cggcaagggt gccaacctgg
     2641 gcgaactgag ccacgcgggg cttcccgtac cacctggctt cgtcgtaacc accacggcct
     2701 acgacacctt cgtcgaggca aacgggatcg gagacgcgat agtcgggcgc gccttgacga
     2761 cccgggcgga cgacccggtc gggttcgagg aggccgcgga ggcgatccgc gccctattct
     2821 ccggcggcaa ggttcccgag gagctggcgg acgagctacg cgccgcctac caggagttgg
     2881 gcgaagatgg agagacgccc gtcgccgtcc gttcctcggc caccgccgag gatctgcccg
     2941 ggatgagctt cgccggccag caggagacct acctgaacgt tcgcggagcg gaggcgctgc
     3001 tcgatggggt caagaacagc tgggcttctc tgtggacggc gcgagcgatg gcctacagga
     3061 agcgccaggg cgtagacccg gcgatcgtga gcctcgcggt ggtggtccag aggatggtcg
     3121 agtcggaggc cgcgggcgtg atgttcaccg ccaacccctc gaatggccgc cgggatcagg
     3181 cgacgataag cgccgcgtgg ggactggggg aatcggtggt gagcggctcg gtgacgccgg
     3241 attcgatcgt cgtggataag gggagcgggc tcgtgctttc gcgcgagacc gccgacaaag
     3301 aggtcatgac cgtctatacc gagggtggca ccgcagagag gcctgtgccg gaggcgttgc
     3361 gcaggcagcc ggttctcgac gacgaggcga cggtcgcact gacccgctac ggggcaacga
     3421 tcgagcataa ttacggattt ccgcaggaca tcgagtgggc gttcgcgggc ggtgagttct
     3481 ttatcgtgca gtcccgcccg ataaccgccc tgcccgatcc gatggccgat ccaccgactg
     3541 actggagcgt gcctatcccg aagggcacgt actggcgcgc cagcatcgtc gaacagatgc
     3601 ctgacccgct ctcgccgctg ttcgccgatc ttgcctccgg agcggtcccc cgctcactgg
     3661 acaagctcat ggaggagtta ttcggctcca gcgtcttccg ggagggtgac ctcgcattcc
     3721 ccacggtcaa tggctacgcc tactactact attcactcgg cgcgttctgg cgcctcctcg
     3781 cgaaaacccc cacggcattc cggctcttga tggaaagtgg ggatggtggc gcagtagtgc
     3841 gctggcgcga gtacgcccga ccgcgctacg cgaggacggt ggcagagtgg gaagcgaagc
     3901 cgcccgaaga tctgcaggcg gaagaactcc ttgctggcgc gcggaaactc ctggatgcag
     3961 ggaccgagta ctacacttcg gtgcagacca tcatcccgct cgcctccatt agcgaggcgc
     4021 tgctcaccat gtattacgac cggctcgtca ggcgtgatgg agatcccccg gcacagacct
     4081 tcctgctggg cttcgacagc atgcccatcc gggccgagaa gtcgctctac gatctcgcca
     4141 cttggtctcg cgagcatccc gacctcgccc tggcgctggc ccgcacaccc tcagaaagga
     4201 tcctcgatct gctcgggacc gaaggggcgc cccccggcgt ggacacagaa gagtggggcg
     4261 agtggcggtc ccgcttccag tctcacctgg atcgctacgg gcatgccgtc tacaacctgg
     4321 acttcgtgaa cccggtcccc gccgacgacc ccacgcccct cttcgacacg ctcaggttct
     4381 acctccgggg tgaaggacgg aacccccacg agcgccagag ggggacagct acacgccgtg
     4441 aggacgcttc gagggcggtt ctggagcggc tggacgcccc gcgaggaaac ctctttagca
     4501 gattgctcta ttgggcgcag agtgttgcgc cggtccgcga ggacgccctc gccgacgttg
     4561 gcctggcctg gcccgttatg cgccggatgc tcctcgagct gggccgccgc ctggtagagg
     4621 ccggagccgt agaaattccc gccgacgtgt tctggctgag ctgggacgag ctggaggaca
     4681 gggccgggtc attggacgcg ggcgaggcgc aactcgcaag cctggccaag cccgtcgagc
     4741 agcgcaagat gctctggcgg ggacagagac gggtcacgcc accgcagctg ctgccgaggg
     4801 gaacctggct cgaggtcttc caacgtctta tgccggcggc atccgaggag cagaccggcg
     4861 acaccatcaa gggtgttgcg gccagcgggg gccaggtcac agccccggcc cgcgtcctct
     4921 ctggaccgga ggacttcggc cagatgcagc cgggagacgt gctggtggca ggcatcacca
     4981 ccccggcatg gacgtcgctc ttcgccatgg cctctgccgt agtcaccgac gtcggcggcc
     5041 cattgagtca cagctctata gtagcccgcg agtacgccat ccccgccgta ctgggtaccg
     5101 gcgttgccac caggcgcatc cgctcgggcc agagtgttcg ggtcgatggg gatgcgggca
     5161 cggtaacgct tctggatgta cccggcgcta cggacgccgg gcagtcgctg ccacaacctg
     5221 tgctcgcagc gcagggcctg gacgcgggca cgaaaatggt tgccctcttt gcactggcca
     5281 tcggagcggt ggtagtagcc gccatgtggt ggagaaagcg gaggcggagc tagaagtcag
     5341 gtttcacttc ctccttggga cgacgcgacg gaacccgtag acttgagatc cgctcccgga
     5401 agggaggcgg atagtcttgg atgtcgactt ctggggaggg tttggatggc cgaggtggtt
     5461 ctgttccatt ccgtactggg actcagcccg ggggtgttct ctgctgccga aaggctaggc
     5521 gccgcggggc acaccctcca caccccagac ttcttcgacg gggaagtttt cgacgacctc
     5581 gaggagggca tgcgcaagga ggaggcccta ggcttccaag agatcgcaag gagggctagg
     5641 gattctgtcg ccggtttgcc ggaggggctg gtcttctgcg ggttctccct cggggccgtg
     5701 cacgccgaag cgctggccgc ctccaggcca ggcgccctcg gggcggtact catggccggc
     5761 gccgtccccg tagaggcact cgaggagtac ttcggcatag atcgctggcc cgaaggggtg
     5821 gcggtacagg tacactacgc cgcagatgac ccctgggtgg aggtcaagga ggaggttgta
     5881 cccctaggcg aggccgtcag aggagccggg gccgccttcg aggcgcacag ctaccccggc
     5941 tcgggccacc tgttcttcga gcctggggta cccgtctacg accgcccttc ttcggaggag
     6001 atgtgggaga gggtgctcgt tttcctggat cggatcgacg cccgagcgtg ggacgtcgaa
     6061 ggggcccggt agccggcttc ggcgccgtag cgaagccagc ccgcacctcc gttcatggat
     6121 cttgttcgga tgtacggttc cgcgccgtgc tatcggcgta tggcggcgag cggttggagt
     6181 cggcgaggac ttggcgacgg ggctcagccg ctagaatcgg tgtgtgcctg aacagggagg
     6241 atgcgcccgg cgttgcatcg ctacaatggc tcagtcggct ccgatgtctg gaggtaggtg
     6301 atgggtgggt tgctggtcca gtttctgggt ctggtcgcgc tgttgggcct cgttgggtgg
     6361 ggctaccggc gctggatcag cccccgccgg gttggcatcc agggcaaagg gatgctgctg
     6421 ctatgcgtcg tgactctcgt aggcggtttg ctcggcagca caggatggtg gatcgacgac
     6481 ccgagagcct tctcgtggga cttgccgccc gtcgccagtc gcatgctggc ttccgcgggc
     6541 tgggcgttcg gcgttgccac gattgcggcc ctgcaacggc ccgttcccca ccgtatgcgg
     6601 ttggtgatgc tcatgctggc cgcgtacctt gtgccattgc ttctcaccgc tcccctgttc
     6661 cacctcgacc gcttcgaccc tgctgcgccc atcacctacg cgttcttcgc gctggttctt
     6721 actatgaccg ttgccgcaac ttggtatctg gtcaaacagc ccgttgtcgt ttccgatgag
     6781 ccggccgatt cgctcgctcc cagggcgctc gtcagtaact ggctcggtct cgtggcggcc
     6841 gtgtgcgcct tgtggggatt ggcgcttttc gttaccgacg atgggccttc gggtttgatc
     6901 tgggtgtggc cgggtgacct tctcactagc cggctcatag cggtgatgct cttgaccatc
     6961 gccttgggcg ccgtgtacgc cctgcgctac gccgatatag cgagggtgat gctgggcgta
     7021 atagcggttt acggcttcgg ggtaatgctg gccaacctgt ggaacatcct tgcgaacttg
     7081 cccatcaatc tctcttatgt ttcagtattc ggcatcatgt ttgtcacttc cgccacccta
     7141 ctccttgcgg agaggtagaa catcgtcaag aggtccggga ctactcgagc cagccgtcat
     7201 tcccgcgagg cctgtccccg cgaatgcggg gagcggaaat ccaccgcacc agacgctacg
     7261 ctcctccgat cggtagcgat ctaccagatc gcgtacgtta cacacttcgg cccatgacac
     7321 tccatctggc aagcttgata atggctgaag actcgacccg aagttatatg gccccccata
     7381 cgcttcgggc cgagttttcg agtgtaagag ttcgcgcaaa atactctaac gcctgcgcgg
     7441 agctggggac ggcaagaacg ctatcggccg acgggccatg ccgtagaatg cgaaacatga
     7501 cagaatatcc ggccgttcgc atcatcgtcg cgccctacga ctccgggttc cgcggcctca
     7561 ggatgggcgc cggaccagac tgcctcatgg aaggcgggct cccggagatg cttcgctcca
     7621 ccgaacggcc ctctttatca ttcgtgcgcg tgctccccga ggtcgacccg ccggccgagg
     7681 tggcagttgc cttcgagcta gaccgcctgg tcgccgcgca ggtgggggag gccttagaag
     7741 gtggcgagtt ccccctggtg ctctccggaa actgcaatac tgcagtgggg acgatctcgg
     7801 gtctcggcgc gtcggacttg ggcgtggtgt ggttggacgc ccacgcggac tttaacaccc
     7861 cggagacgac cactaccggc ttcacggacg gaatgggcct cgccgtagcc gtagggcatt
     7921 gctggaaaga gatggccagg ggcgtgtccg gcttctccgc ggtggccgag cgagacgtgg
     7981 tgctggcggg ggtcaggacg gtagagcctg ctgaggagca acgtctcg
//
LOCUS       DATMVF010000305        22485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_269348, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000305 DATMVF010000000
VERSION     DATMVF010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22485
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269348"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VN178_15970"
     CDS             <1..475
                     /locus_tag="VN178_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HWS82516.1"
                     /translation="GMAARLSADQLADAPGLADRADASRRRVAPLASTDAEAYGRVLE
                     AYRLPREPDSETRAERIKVALSGAADVPLAIAEAGSEVAGIAARLVEEGNPNLEGDAM
                     TALLLADAGVRSAAALAELNLSSANLEDDRLGRAKELVDETAATVRRVTEEHGSG"
     gene            534..1835
                     /locus_tag="VN178_15975"
     CDS             534..1835
                     /locus_tag="VN178_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HWS82517.1"
                     /translation="MPERRTPFYEFHRRAARNLVKGGGDFMFPTSYASSLEEHLNVRR
                     NVGMQDLSTMGEVDIKGPGAERLVRRLLVNEVQDMEPGQLRYSTMCNEAGGIVDDVTV
                     YKFDDEHFMIVTSSGPRKKAFRWISEHAVGSSAYVTDMTAAVALPVVQGPRSRAFLKT
                     VVEGVDLDELRFFRFAPCRIGDIELFISRSGYTGELGYELYTPADQAGVLWEYLLEKG
                     RAFDLRMYGVEAMQSLRIEKGLPLYGPDISEQETPFHVGLGRWVRFDKRDFVGREALL
                     RVQERGLERRWVGLTLESEVPASAGAGVYGIADVATFREFVETGAEAGEQEDEFMPGE
                     RQVGRVTSSAMGHTVGRMLAMAYLDTAHSWPGNNLIVAFGGRPVPARVAQTPFFDPEN
                     ARVRARPHEDDQLSELVPEPARLTNARPKTPVRQSPNGNTR"
     gene            1836..3101
                     /gene="solA"
                     /locus_tag="VN178_15980"
     CDS             1836..3101
                     /gene="solA"
                     /locus_tag="VN178_15980"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWS82518.1"
                     /translation="MNVQYSGRYDAIVVGVGGMGSATAYYLARRGRRVLGLERFGIPH
                     TMGSSHGHTRIIRLAYYEDPSYVLLLRRAYELWREIQGKSGEKLLHITGSIDAGPEES
                     WVFKGSWESCKLHDLPHEVVTGAELGRRHPGYHLPPEHLALVQPEGGFLTPERCIVSY
                     VMAAQSLGAEIHGHERVLEWVPLEGGVRVRTDRGTYEADRLVMTAGAWNGDLLDVLGG
                     LAVPERQVLAWLQPSRPDLFRPDTFPVFNLLVDEGRFYGFPVYGVPGFKFGKYFHLGE
                     SGHADEIDHQPHDYDEQLLRNFAERYFPDGCGPTMDLQTCMFTNTPDHHFVIDLHPDY
                     PQVSIASPCSGHGFKFASVVGEIMADLAEKGFTRHDISLFRLDRLRRPARQTTGHRSI
                     QPTTAGQPAEQSDGRPRSFSRMGDIRTFW"
     gene            complement(3176..4039)
                     /locus_tag="VN178_15985"
     CDS             complement(3176..4039)
                     /locus_tag="VN178_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWS82519.1"
                     /translation="MDARLSISVVAAVFLAASAFPGISAGLEGYSPGHLALLRFLMAS
                     LVLAVYAVFSGIRLPAARDLPAVALAGFLAFSVYNVLLGYGQTSVPAGTASLLIASIP
                     AFTALWAVAFLDERLGARGWAGIVVSFLGVALISFGKGEGFGADPGALLVLLAALSAS
                     GYFALQKPYLERYGAFEFTSYAIWAGTLLLLPFAPGLLEQTRQTTLEPTLAVVYLAIS
                     TPLSYATAAYAFSRLPASRAVTLESLIPPAAILIAYLWLGEVPTLLSLAGGAVAILGV
                     VLVNTRAEKIE"
     gene            4277..5050
                     /locus_tag="VN178_15990"
     CDS             4277..5050
                     /locus_tag="VN178_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWS82520.1"
                     /translation="MCALVRRSSSVQSVDRAVSVLEFLSRRGWSGVTEVARELEIHKS
                     TAYRLLTTLRDRGLVEQDAATEKYRLGFGLVLLARAVRADLDILRCARPVSERLSERT
                     RETVTIAVLEGDDAVVIHQSISRASALGVDWTGRHTPLHATAAGKIFLAYMPEDQLLR
                     ILEGPLERFTDNTIVDPASLKGHTRKILDAGFGYTVEELETGLNAVGAPIRGADSTVV
                     GAVSVSGPAFRLPPDALPEVGELSIRTAAEISRCLGFQG"
     gene            complement(5186..6472)
                     /locus_tag="VN178_15995"
     CDS             complement(5186..6472)
                     /locus_tag="VN178_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWS82521.1"
                     /translation="MQTENPVYEEIQEILRAVEIPSVALVGQRIDSPPALANIREGVR
                     EALNSVELPSGSVAIGVGSRGVARIGEVVAALAEALKDSGAEPFVVPAMGSHGASTAE
                     GQAQVLAHLGVSEEQVGCPVRATMESVRLGETPSGVAVYMDRNAYEADSVVVVNRVKP
                     HTAFRGAVESGPTKMLAIGLGKQRGAHSIHSAGWENIHQTIPEAARVAVESGKVAFGL
                     ATVENAEEEPCKIVAIPAGELEEAEAPLLEEAKRNLARLPFDEIDVLIVDEIGKNISG
                     DGADPNVTGRYPTTAASGGPSVERMVYLGLTEETGGNANGLGMADVVTERLAGSMDRP
                     STYMNALTSTTPAPVKTPMIMPTDEMAVAAALTMCAGVDPAEARLVRIENTLKLGRIW
                     VSEALLEGVEGDEKLDVLEEPQPMRFDRGEIVPRHA"
     gene            complement(6506..6916)
                     /locus_tag="VN178_16000"
     CDS             complement(6506..6916)
                     /locus_tag="VN178_16000"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWS82522.1"
                     /translation="MSGDLQEGGCFCGRVRYRVAGEPLGSAVCHCVSCRRTSGAQSVA
                     WLTFPHKKFSFVHGEPADYRSSDEVSRTFCQSCGTSLTYKHDEEPDSIDVTTASLDSP
                     DGFPPTRHIWLEDKLAWEGVDDGLPRFEQGGPPD"
     gene            7023..7760
                     /locus_tag="VN178_16005"
     CDS             7023..7760
                     /locus_tag="VN178_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWS82523.1"
                     /translation="MRAALFIPCYVDQINPEVGVSVVRVLRRLGVEVIYPEGQTCCGQ
                     PAFNSGFFDEARAIGRRFLDVFERERFDYIVCPSGSCTTMVSHYYPFVFENQALPDDR
                     ERAESLGHRVREFSDFVVNVLGVKDLGASHAGRAVFHTGCHQRRELGILNEPRDLLKG
                     VCGLELLEWENEELCCGFGGMFSVKMPDVSTAMADEKIAALEKSGADTIVSCDSSCLM
                     HLGGRLRRTGHDTRVFHLAEILDSDGR"
     gene            7762..9228
                     /locus_tag="VN178_16010"
     CDS             7762..9228
                     /locus_tag="VN178_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HWS82524.1"
                     /translation="METSKPARRRDTEDRRQEGLESKVEGFNERGRRAVQDRELHDSI
                     SLFTHKTLTGRNAQLRAHPDAPELRERAYRAKQETMANLDYYLSQMADAVEERGGNVF
                     FAADGEDVVRYIGDLARRRGAKILTKSKSMATEEIELNHRLEEDYADLGLEIVETDLG
                     EWIAQLAGDTPSHIVGPIVHMNIERITEILSGVSDEELPPDVESLAAFARKRLREKFL
                     NADIGITGANFGVAETGTICTVTNEGNARLVTSVPPVHVVVMGIEKVIPKFGDLSTFV
                     QLLARSGTGQKITVYTNFITGPRGEGELDGAEEFHLILMDNGRSKLLGTEYEEALYCI
                     RCGSCLNVCPVYRQTGGHAYGSTYSGPIGAVITPLLKGDEEAKDLPYASSLCGACTET
                     CPVGIPLHDLLLKLRNRQVEEGLAGRAESLMFKGFERTMKSPAAYRVGAKVGRLAQKP
                     FVKDGSIEGAPGIHGWTDFRELAPLAEKSFRELWKEGI"
     gene            9231..9899
                     /locus_tag="VN178_16015"
     CDS             9231..9899
                     /locus_tag="VN178_16015"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HWS82525.1"
                     /translation="MADRTEFLESIRHRTRRGRYKPTKSPDSVWTPGVDRPEREELED
                     PPARFLEELEALGGHGKRAGSLEEARDYVLKLAREREAELLVRWDTEYLDELGADGPL
                     REAGVEVALWRDLPDLREIAGRADIGLSTATWAIAETGSLVLEHGPGQGRSVTLLPPT
                     YVAILPADRVLRTIPEAIRKYVGGTLPANVCFHTGPSRSGDIEMSLVVGMHGPGDVHV
                     ILVG"
     gene            9937..10158
                     /locus_tag="VN178_16020"
     CDS             9937..10158
                     /locus_tag="VN178_16020"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWS82526.1"
                     /translation="MIDWSTCPAVERDPERVSGAWVFRGTRVPVAALFENLEDGAQVD
                     QFVQWFPGVSLEQARAVLEHAAESALASA"
     gene            complement(10646..11206)
                     /locus_tag="VN178_16025"
     CDS             complement(10646..11206)
                     /locus_tag="VN178_16025"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWS82527.1"
                     /translation="MNELYVSLAAVGGLLLLLGVLGGPLKERTPVSEPLIALLAGVLI
                     GPAALGLLDLADLGNETLILEQAALVTLGIALVGVALRLPVGYASGNWRLLVVLVGIL
                     MPLMWIVSGLLVHLILGLHFWVSVLIGAIITPTYPVVASSIVAGGVAERNLPSSLRCA
                     ISAESGFNDGLALPFVVLPSRCVRQA"
     gene            complement(11569..12840)
                     /locus_tag="VN178_16030"
     CDS             complement(11569..12840)
                     /locus_tag="VN178_16030"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWS82528.1"
                     /translation="MTTNERVAWRKWFGAAAVLSWATLGLLWAQVILLPKPLLPPPLF
                     AELLGNVPSSTHPQRFIVMIFKEMSLLLTAFALFGLFTVALAHRSGARKSSLVASVLC
                     AATVAVSLVPVAQAWKEASAEDVSLSLLGYFAGSAFAADRSPEIVTYARPGGEELKAD
                     IWRPPGVVADAAKGRPAVIVVHGGGWRSGERGDFPLWNAWLASRGYVVFDIDYRLSPP
                     PSWRDAPSDVRCAVGWVKENAARYGVDADRVALMGRSAGGHLALLTAYSQGSGPTSGC
                     EARSLEDTGVAAVVAFYPPTDLARLSSLGYLGGMDRFLGGTRDTVPERYHLLSPVTRV
                     DSCDPPTFLAHGGDDQIVPPGESELLTERLREAGVPYRLVGPPWANHTFDFIWGGWSS
                     QITRSTLQEFLEAHLETPRGCHVQTRAGRTG"
     gene            complement(13117..15264)
                     /locus_tag="VN178_16035"
     CDS             complement(13117..15264)
                     /locus_tag="VN178_16035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWS82529.1"
                     /translation="MSIIGGAATYLRAVQSAFGRTAASDGSPAEANRKPAKEGFRPDV
                     EGLRAVAVGVVLLYHGGMPFARGGYVGVDVFFVISGFLITGLLVRELEKTGTISLTRF
                     YSRRAKRLLPLTVVVLGAVVLVVGMWPQFDPVRMDEVSLGVVASGLYVMNWLLAARAT
                     DYFAAGLQASPVQHFWTLAVEEQFYLVWPALLLGVARWCSRIGLGLRPALATAFAAVA
                     ISSLAYSVYSTELQAGAAYFSTLTRGWELALGGMLALVPASRLGQLPRWAAFALAWVG
                     LGAIAFATFRFSDGTLFPGYAALVPTLGTAAIIAAGFESTGTSAGPARLLTLGPVRHV
                     GRISYSWYLWHWPPLVFAAAIWGRLSPAEGLVVLVASYVPAVLTNRLVEKPFLHSKTL
                     TRFPRKALALGGACTVSSVALGVGLFALTPTLPEAPESQVAGAAALQDDDSLQKSAKA
                     VHPTPREAETKENRPLMYADGCHLNPPETEVPRCVYGNPSSRTTVVLFGDSHAMQWFP
                     ALNELARERNWRLVGLTKAACPPAEVHIYNASLRRVYRECVEWREQMLERIVQEENPS
                     LIVTSSLPTYRPREEGKRLPRDASGEAMVEGYASTLKKLRGTGAPVALIEDVPHPNKN
                     VPECVSRSLDHLQRCASPRSEALDYPKVNTRVAEEVEGVYLIDPTPVLCLEKACPAVI
                     GDVLVYRNGAHLTATYVRTLTPWLGNQLPEPAG"
     gene            complement(15261..15689)
                     /locus_tag="VN178_16040"
     CDS             complement(15261..15689)
                     /locus_tag="VN178_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82530.1"
                     /translation="MTEDKKTQQNRRPPVRRPASTWWLLAKGKSLGAGALIADCGGAQ
                     TLLVFSGEGEAEVFAWLGGAYEDGWQVRATSAGELVSILYGPCAGVGRVALDPSPVMS
                     PETIRLVSLSRERFVGWIVDSPCSYSPGERHDARGLARLL"
     gene            complement(15696..16607)
                     /locus_tag="VN178_16045"
     CDS             complement(15696..16607)
                     /locus_tag="VN178_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWS82531.1"
                     /translation="MMNATLTKILLATDSSRDAALAMRAGADVSCKTGAQLHVVHVIP
                     DVLPPSNPGSTIFDDSSHLAEEEARELLRKQAWRARVEGGEVAGEHLREGRPAQEVNT
                     LAAELNVDLVVVGSRGAGWVKRLVTGSVSEDLVHRATCPVLIVRGREDAWPPARVVVG
                     DDGSEVAERTDILAAKIASLFGAEVVLVRAYKHPPEPVGGWSAQGRRELDEALRRRRE
                     DLEKRAEQLAALTHQRIGTRLTETKPVPAMTLVAGKRDEGRTLLAVGSRGLGALDRVL
                     SGSVSTKVLRAAHGPVLVVPSENAPSR"
     gene            complement(16632..16889)
                     /locus_tag="VN178_16050"
     CDS             complement(16632..16889)
                     /locus_tag="VN178_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWS82532.1"
                     /translation="MGLISWIIIGAIAGVVARRIVPGPDPGRFFVTVVLGMAGASLGG
                     FVMGVLGGSGTTSLDVWSVLVATLGAVMSLYLYSLIVRRTA"
     gene            17042..17776
                     /locus_tag="VN178_16055"
     CDS             17042..17776
                     /locus_tag="VN178_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWS82533.1"
                     /translation="MMRILIVEDEEHLARLLAEVLGREGYAANTVSDGRSGLTRALSE
                     DFDLLVVDWMLPDLDGVQVVKRLRTADVRVPILMLTARAQVEDRVEGLDAGADDYLPK
                     PFAFPELLARVRALSRRPPEPETEGTVLTAGDVALDPIRHEVRRAGERVDLTAKEFAL
                     LATLMQRPGQVFTRSVLLDTVWGGTTGAYTNVVDLYIHYLRKKLDAGEEPSRIRTVHG
                     TGYTFDPRPEYPGQGSSQGQASIEET"
     gene            17776..19029
                     /locus_tag="VN178_16060"
     CDS             17776..19029
                     /locus_tag="VN178_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWS82534.1"
                     /translation="MLERMRWRLTMGYAGIFALILLVLATAAVVGFSRELTNQQDTLL
                     TQEAKDLESNLLDGDRREVLAEGSAEYSWVALDPEGHVTDRDPTAATLGTLGLPSKEL
                     ARQSLEQNERVSATIRGPKGRVRVVSMPMREESGEVVGIVQYARSLKGVQQTISRLVL
                     VLLPLGLGGLGAALLGGLYMAGRAMRPARESFQKQRAFVADASHELKTPLTLIRADAE
                     MVLYRGHLNKEDRKLVEHALVETDRMSAILSDLLLVARLDAGKLEVSEKPFDLTAVLS
                     EEAERFGVRAAAKEIQLEVRVPGELPARGDPKRTGQIIGVLLDNAVRFTPPGGSIAVA
                     GRLHDRWVEASVTDTGPGISPEHLPRVFDRFYRAETSRTRGRGGGMGLGLAIARALAR
                     AQGGDLVADSAKDGGATFRLRLPRE"
     gene            19516..20808
                     /locus_tag="VN178_16065"
     CDS             19516..20808
                     /locus_tag="VN178_16065"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HWS82535.1"
                     /translation="MITTQAHDDTAPGYIFAALKEGAGEHGPMIIDDQGELVWFGKYR
                     SARDFKMQYYKGRPVLTWWEGKVTAGHGIGEYVIFDGSYREIARVRAGNGYRGDLHEF
                     LITPEDTALLVAYAPRRTDLSPLGGPKDGMVWEGIVQEVNIQSGGVIFEWHSLEHVGI
                     EESYIEPSEHPDHLYDYFHINSIDVEPDGNLLICARNTWTVYKVERESGEIIWRLDGK
                     KSDFEMCTGTQSAFQHDARRQSDGTITIFDNGAHPKVHDQSRGIVVELDELKMSARLV
                     REYTSPKKRLSTSQGNVQLLSNSNILIGWGSEPFISEFSREGELLFEARFPPGGESYR
                     AFRFPWSGHPTDEPAVTVEQGPDDKLKLYASWNGATEVESWEVFAGPRLGRLGSVGSV
                     PRDGFETAMLVQTSHSYVAVRANDRLGRPLGASAPVKL"
     gene            20855..21064
                     /locus_tag="VN178_16070"
     CDS             20855..21064
                     /locus_tag="VN178_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWS82536.1"
                     /translation="MAKGTVMWFSEEKGYGFISPNNGGESIFVHYTDIVGSGFRSLKV
                     GEIVSYVPARGPRGRVVARTVHRTE"
     gene            21264..21716
                     /locus_tag="VN178_16075"
     CDS             21264..21716
                     /locus_tag="VN178_16075"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWS82537.1"
                     /translation="MTHPSRAGIGLVRLGESDFVPAKPENDLRGKDVYDPEGQRIGGV
                     KDLYIDPQEREVRFLEIGFGGLLGIGEKPILVPVEAVTRITEARVTIEPGRTEKVEGP
                     APFDTTVASPRTDGPREDHTSPRWDNAERIADQRRYIHSSLPYGSWPF"
     gene            21795..22115
                     /locus_tag="VN178_16080"
     CDS             21795..22115
                     /locus_tag="VN178_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82538.1"
                     /translation="MWGVRGILSKDILPDRPADRRRRSRLCALRAMDRLGMLGEENLI
                     PTLSRRPQLRWLADEEGARWAVLAELGRIGEPDTFEEAVGWALENRPSPEEAKAYVRR
                     IRTC"
     gene            22264..>22485
                     /locus_tag="VN178_16085"
     CDS             22264..>22485
                     /locus_tag="VN178_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82539.1"
                     /translation="MVTPAAIGVILILIGLVLFVVDLSVTNHGLLTAGGIVTLLAGGM
                     VLLGTGVPYSGVLLGAVVVVAISMGGVLLG"
BASE COUNT         4302 a         7068 c         7243 g         3872 t
ORIGIN      
        1 gggcatggcc gcccgcctgt ccgccgatca acttgccgat gctcccgggt tggccgaccg
       61 tgcggatgcc tcgaggagga gggtcgctcc tctagcaagc accgacgcgg aagcctacgg
      121 ccgcgtgctc gaagcctatc gtctcccgcg cgagcccgac tccgagactc gcgcggaacg
      181 catcaaagtt gccctctcgg gggccgccga cgtgccgctt gccatagccg aggccggcag
      241 cgaggtagcg ggcatcgcgg cccggctggt ggaggagggc aaccccaacc tcgagggaga
      301 tgcgatgacc gcactcttac tcgccgacgc cggtgtacgc tccgcagcag cgctggccga
      361 gttaaacctg tcttcggcga acctggaaga cgaccggctc gggcgggcaa aggaactcgt
      421 cgacgagacc gccgcgaccg tacggcgggt gacggaagag catggatcag ggtgaacatg
      481 atgagagctt gtagtatagt ttacatacaa cgcttctaag agaggggtag ctcatgccgg
      541 agagaaggac gccgttctac gaattccacc gccgtgcggc caggaacctg gtcaagggag
      601 ggggggactt catgttcccc acctcctacg cttcgtcttt ggaggagcac ctgaacgtac
      661 gcaggaacgt ggggatgcag gacctatcga cgatggggga ggtggacatc aaggggcctg
      721 gcgccgagcg gctggtgagg cgtctgctgg tgaacgaggt ccaggacatg gaacccgggc
      781 agctaaggta ctcgacgatg tgcaacgagg ctggcggcat cgtagacgat gtcacggtct
      841 acaagttcga cgacgaacac ttcatgatcg tcaccagttc cgggccgcgc aagaaggcgt
      901 ttcgttggat ctccgagcac gcagttggtt ctagcgccta cgtgaccgac atgacggccg
      961 ccgtcgccct gcctgtggtc cagggaccgc gttcgcgcgc cttcctcaag accgtggtcg
     1021 aaggtgtgga tctcgacgag ctgaggttct tccggttcgc gccgtgccgg atcggggaca
     1081 tagagctgtt tatctcacgc tcgggctaca ccggcgaact gggctacgaa ctgtacacac
     1141 cggcggacca ggccggggtg ctctgggagt acctcttgga gaagggtcgc gcgttcgacc
     1201 tcaggatgta cggcgtcgaa gccatgcagt ccttgcgcat cgagaaggga cttcctctct
     1261 acggccccga cataagcgag caagagaccc ccttccacgt gggtctcggt cgctgggtcc
     1321 gtttcgacaa gcgcgacttc gtaggccgcg aggccctgct gcgggtacag gaacgcggcc
     1381 tggagcgacg ctgggtcggc ctgaccctgg agagcgaggt ccccgccagc gcgggcgccg
     1441 gggtctacgg catcgccgac gtcgccacct tcagggagtt tgtggagacc ggcgccgagg
     1501 cgggcgagca ggaggacgag ttcatgcccg gcgagaggca ggtcgggcgc gtaacctcga
     1561 gcgccatggg gcataccgtc ggcaggatgc tggccatggc gtatctagac accgcccact
     1621 cctggcccgg caacaatctg atcgtcgcgt ttggcggcag gcccgtcccc gcaagggtcg
     1681 cccagacgcc cttcttcgac ccggagaacg cgcgcgtcag ggccagacct catgaagacg
     1741 accagctctc cgaactggtc cccgaaccgg cgcggctgac gaacgccaga cccaagacgc
     1801 ctgtgagaca gtctccgaac gggaacacca ggtaaatgaa cgtccagtac tccggtcgtt
     1861 acgacgccat cgtcgtcggc gtggggggga tgggcagcgc cacggcctac tacctcgcca
     1921 ggaggggcag gcgggtgctt ggcctggagc ggttcggcat cccccacacg atgggctcct
     1981 cccacgggca cacgcgcatc atccgcctgg cctactacga ggacccctcc tacgtgctgc
     2041 tcctcaggcg cgcctacgag ctgtggcgtg agatccaggg caaatccggc gagaagctcc
     2101 tgcacatcac gggctccata gatgccggac ctgaggaatc ctgggtcttc aaaggctcgt
     2161 gggagtcttg caagctccac gacctcccgc acgaggtggt gacgggcgcg gaactcgggc
     2221 ggcgccatcc gggctatcat ctcccccccg aacacctcgc cctcgtccag cccgagggag
     2281 gtttcctcac gccggagcgg tgcatcgtct cctatgtgat ggccgcgcag tccctcgggg
     2341 ccgagatcca cggccacgag agggtcctgg agtgggttcc gctggaggga ggggtacgcg
     2401 tccgtaccga ccgtggcacg tacgaggccg acaggctagt gatgaccgcc ggcgcctgga
     2461 acggggatct cctcgacgtc ttaggtggcc tcgcggtgcc agagaggcag gtcctcgcct
     2521 ggctgcagcc ttccaggccg gatctcttcc ggccagacac cttccccgtc ttcaacctgc
     2581 tcgtggatga gggccgcttc tacggcttcc cggtctacgg cgtgcccggc ttcaaattcg
     2641 gcaagtactt ccacctgggc gagtccggcc atgccgacga gatcgaccac caaccccacg
     2701 actacgacga gcagctcctc aggaacttcg ccgaacgata cttccccgac ggatgcggcc
     2761 ccacgatgga tctgcagacc tgcatgttca ccaacacccc ggaccaccac ttcgtcatag
     2821 acctgcaccc cgactacccg caggtctcca tcgcctcgcc ctgctccggg cacggcttca
     2881 agttcgccag cgtcgtaggc gagattatgg ccgacctggc cgagaaagga ttcacccgtc
     2941 acgatatctc cctcttccgc ctcgaccggc tgaggcggcc ggcacgacag accacaggcc
     3001 accgatccat ccagccgaca accgcagggc aaccggccga gcaatccgac ggtcgtcctc
     3061 gctccttcag caggatggga gacataagaa cgttctggta gccgcgggct acggcacttc
     3121 gcgaaaagac cgatcctcgg ccgaggggag tcgcagaacg ctcttctccg actcgctact
     3181 ctatcttctc ggcccgcgtg ttgaccagta ccacgccgag aatcgccact gccccaccgg
     3241 ccagggacag gagggtaggc acttccccga gccagagata agcgataagg atcgccgccg
     3301 gtgggatcag ggactcaagg gtgaccgctc tggaggccgg taacctggag aaggcgtagg
     3361 cggcggtggc gtaggagagc ggagtggaga tcgccaggta gacgacggcc agtgtcggct
     3421 ccaacgtcgt ctgcctcgtc tgttcgagga gcccgggcgc gaacggcagc agcaagagcg
     3481 tccctgccca gatggcgtaa gaggtgaact caaaggcccc gtacctctcc aggtagggct
     3541 tttgcagggc gaagtagccg ctggcagaga gcgccgcgag cagcaccagg agggctccgg
     3601 gatcggcacc gaacccttcg cctttgccga aggagatcag ggctactccc agaaaactca
     3661 ccacgatccc cgcccaccct ctcgccccga gccgctcatc gaggaacgcc accgcccaca
     3721 acgcggtgaa ggccgggatc gaggcgatca agaggctcgc cgtccccgcc ggaacgctcg
     3781 tctgcccgta gcccaagagc acgttgtaaa cgctgaaggc cagaaagcct gccagggcca
     3841 ccgccggaag gtccctcgcc gcaggaaggc gtatccccga aaacacggcg tagacggcga
     3901 gcacgagcga agccatcaag aaacgcagaa gggcgaggtg cccgggggag tagccctcga
     3961 ggccagccga gatcccaggg aaagccgagg ccgcaagaaa gaccgcagcc accaccgaga
     4021 tcgagagtct tgcgtccatc cacgaggcca cgaactccct caccaactcg aaactacgcc
     4081 tacgcggcgt tccttggcgt ctttgccaac tatcttacac gcaacgagtg taagggaagc
     4141 ggggcgtgcg gcacgatctc cgaggtcggg cgctaccggc agagcaagca ggcgggctag
     4201 taggcacgaa gcgcgtagac tctgagcgcc gggtggaccg agccgagata ccgaaggtag
     4261 tgtaggatct gcgcttatgt gcgccttggt acgtaggagc tcctcggtac agtcggtcga
     4321 ccgggccgtc tccgttttgg agttcctgtc gcgccgcggg tggtcgggcg tgaccgaggt
     4381 cgcaagggag ctcgagatcc acaagtctac cgcgtaccgg ctgctcacga cgttgagaga
     4441 ccgggggttg gtagagcagg acgcggcgac cgagaagtac aggctgggct tcgggctggt
     4501 gctactggcg agggcggtga gggcagacct cgacatcctt cgctgcgcca ggccggtcag
     4561 cgagcgtttg agcgagcgta ccagggagac cgtcaccatc gcggtcctgg aaggtgacga
     4621 cgccgtagtc attcatcaga gcatctcgcg ggcttctgcc ctcggcgtcg actggacggg
     4681 acgccacacg ccgttgcacg ccacggctgc ggggaagatc tttctcgcgt acatgccaga
     4741 agatcaactc ttgcgcatcc tggaaggacc gctggagcgc tttaccgaca acaccatcgt
     4801 cgacccggcg agcctgaaag gccacacaag aaagattctc gacgcggggt tcggatatac
     4861 cgtcgaggaa ctggagacgg gactgaacgc cgtcggggcg ccgattcgcg gtgccgacag
     4921 cacggttgtg ggcgcggtga gcgtgtcggg ccctgccttc cgtctgccac ccgatgctct
     4981 ccccgaggtc ggcgaactat caatacggac cgccgccgag atctcccggt gcctgggatt
     5041 ccagggctga accgggctgc ggaggctctc gactaccgga aaggggagag gcgtgagtgg
     5101 ttaccaaggt ttcggaagca gctccccgtc gaacagcgcg tggaccgtgt aaatccctgc
     5161 cacctgaagt ctgatacctc gagtgctacg cgtgtctggg gacgatctcg cccctgtcga
     5221 agcgcatcgg ctggggttct tccagaacgt ccagtttctc gtcgccctcc acgccttcca
     5281 gcagcgcctc tgacacccag atcctcccta gcttgagggt gttctctatg cggacgagcc
     5341 tggcttcggc ggggtcgacg ccggcgcaca tggtaagggc ggcggccacc gccatctcgt
     5401 ccgtcggcat gatcatgggc gtctttacgg gcgccggcgt ggtcgaggtg agggcgttca
     5461 tgtaggtaga gggacggtcc atcgagccgg cgagacgctc ggtgacgacg tccgccatgc
     5521 cgaggccgtt cgcgttgcct ccggtctcct ccgtcaggcc gaggtagacc atgcgctcca
     5581 ccgaagggcc gcccgaggcg gcggtcgtcg ggtagcggcc cgtgacgttg gggtcggcgc
     5641 cgtcgcccga gatgttcttc ccgatctcat ctactatgag aacgtcgatc tcgtcgaagg
     5701 gcagacgcgc taagttgcgc ttcgcttctt cgagcagcgg cgcttcggcc tcctcaagtt
     5761 ccccggctgg gatcgccact atcttgcacg gctcttcttc tgcgttctcg acggtggcga
     5821 ggccgaaggc gaccttgccg ctctcgacgg cgacgcgggc cgcctcgggg atggtctggt
     5881 ggatgttctc ccagcccgcg gagtgtatag agtgcgcccc ccgttgcttg cccagcccga
     5941 tggcgagcat cttcgtcggc ccgctctcca ctgctcccct gaacgcggtg tggggcttga
     6001 ccctgttgac cacgacgacc gagtcggcct cgtaggcgtt acggtccatg tagaccgcta
     6061 cgcccgaggg cgtctccccg agcctcacgc tctccatcgt cgcccgcacg ggacacccga
     6121 cctgctcctc gctcacgccg aggtgcgcga gcacctgcgc ctggccttcc gcggtcgagg
     6181 cgccgtggct ccccatggcg gggacgacga aaggctcggc gccggaatcc ttcagggcct
     6241 cggccagcgc cgcgactacc tcccctatcc gtgcgacacc cctgctaccg acgcctatgg
     6301 ccacagagcc agagggtagc tccacggagt tcagcgcctc ccggacgccc tccctgatgt
     6361 tcgcgagcgc cggcggagaa tctatcctct gcccgacgag cgcgacgctg ggaatttcga
     6421 ccgcgcgcag gatctcctgt atctcctcgt agacggggtt ctctgtctgc actgcctcac
     6481 ctccgtagag accaccttta tagcgctaat ccggcgggcc accttgctcg aaccggggaa
     6541 gaccgtcgtc gacaccctcc caggcgagtt tgtcttccag ccatatgtgg cgtgtgggag
     6601 ggaacccgtc gggactgtcc aggctcgctg tggtcacgtc gatcgagtcg ggttcctcgt
     6661 catgcttgta agtgagcgac gtgccgcaac tctggcagaa cgtcctgctc acctcgtcgg
     6721 aagatcggta gtcggccggt tcaccgtgga cgaaggagaa ctttttgtgg gggaaagtga
     6781 gccaggcgac cgactgtgcc ccggaagtcc tgcggcacga tacacagtgg cagaccgctg
     6841 accctagagg ctcgccagcc accctgtatc tcaccctccc acagaaacat ccaccttcct
     6901 ggagatcacc gctcatccct tctccctact cggtagccgc gaccattcta acagggcatc
     6961 gtggagccgc gtctgttggc gttgctatac tggccttcga aatcgtcggg gagaggagcc
     7021 cgttgagagc agcccttttc ataccgtgct atgtggacca gatcaatccc gaggtgggcg
     7081 taagcgtggt gcgcgtactg agaagacttg gggtggaggt gatctacccc gagggtcaga
     7141 cctgctgtgg gcagcctgcg ttcaattccg gcttctttga cgaggcccgc gccataggac
     7201 gccgctttct agacgtcttc gagagggagc gcttcgacta catagtgtgt ccttccggat
     7261 cttgcaccac catggtctcc cactactacc cgttcgtctt cgagaaccag gctctgcccg
     7321 acgaccgcga acgcgcggag tctctcggcc accgggtcag ggagttctcg gacttcgtcg
     7381 tgaacgtgct cggcgtaaag gacctgggtg ccagccacgc cgggcgcgcg gtcttccaca
     7441 ccggctgcca ccagcggcga gaactcggga tcttgaacga gccgcgagac ctgctgaagg
     7501 gggtctgtgg cctggagctt ctggagtggg agaacgagga gttgtgctgc gggttcggag
     7561 gtatgttctc cgtgaagatg ccagacgttt cgaccgcgat ggcggatgag aagatagcgg
     7621 ccctggagaa gagcggggct gacacgatag tttcctgcga ctctagttgt ctgatgcatc
     7681 tcggaggcag gctgcgccgg acggggcacg acacgcgtgt ctttcatctg gccgagatac
     7741 tcgactctga cgggaggtag cgtggagacc agcaagcccg cgcgccggcg cgataccgaa
     7801 gatcgcaggc aagaaggtct ggaaagcaag gtagaaggct tcaacgagcg tggccggagg
     7861 gcggtccagg atcgggaatt gcacgactcg atctctttgt tcacccacaa gacgctaacc
     7921 ggacgcaacg ctcagctcag agcccatccc gatgcgccgg agttgaggga aagggcgtat
     7981 cgcgccaagc aggagacgat ggcgaacctc gactactacc tctcgcagat ggccgatgcc
     8041 gtcgaggaac ggggcggaaa cgtgttcttc gcggctgatg gcgaggacgt ggtcaggtac
     8101 ataggggatc tggcgcggag gcgtggggcg aagatcctca ccaaatccaa gagcatggcg
     8161 accgaggaga tcgagcttaa ccaccgtctg gaagaagatt acgcggacct agggctggag
     8221 atagtggaga cagacctggg agagtggatc gcacagctcg ccggagacac tccctcgcac
     8281 atcgtaggac cgatcgtgca catgaacata gagcggatca cggagatcct ctccggtgtc
     8341 tccgacgagg agctgccgcc tgacgtggag tctctggccg ccttcgccag gaagcgtctc
     8401 agggagaagt tcttgaacgc ggacatcggc atcacgggcg ccaacttcgg ggtcgccgag
     8461 acgggcacta tctgcaccgt caccaacgag ggcaatgcca ggctcgtcac gagcgtccct
     8521 cccgtacacg tcgtcgtgat ggggatagag aaggtgatcc ccaagttcgg ggacctctcg
     8581 actttcgtgc aactgttggc gaggagcgga acggggcaga agatcacggt ctacaccaac
     8641 ttcatcaccg gcccgcgcgg cgagggcgag ctggacggcg cggaggaatt ccatctcatc
     8701 ctcatggaca acggccgctc caagctgctg ggcacggaat acgaagaggc tctctactgc
     8761 atccgctgcg gctcgtgcct gaacgtctgc ccggtctaca ggcagacggg aggacacgcc
     8821 tacggctcga cctactctgg ccctatagga gccgtcataa cgccgctgct caaaggagat
     8881 gaggaggcga aggaccttcc gtacgcctcc tcgctgtgcg gtgcctgtac ggagacgtgt
     8941 cctgtcggca tcccgctcca cgacctcctg ttgaagctgc gcaaccggca ggtcgaggag
     9001 ggcctggccg gacgtgccga gagcctgatg ttcaaaggtt tcgagagaac gatgaagagt
     9061 ccggcggcct acagagtcgg cgcgaaggtc ggcaggctgg cgcagaagcc gttcgtgaag
     9121 gacggatcca tagagggagc gcccggaata catggctgga cggacttcag ggagcttgcg
     9181 ccgctcgcag agaaatcatt ccgagagctg tggaaggaag gcatatagcc atggccgacc
     9241 ggacggagtt cctggagtcc ataaggcacc gcacccgcag aggacgatac aaaccaacga
     9301 agtcgcccga ctcggtgtgg acgccgggcg tagaccgtcc ggaacgagag gagctcgaag
     9361 acccgccggc caggtttctc gaggagctgg aggcgctggg cgggcacggc aaacgagccg
     9421 ggagcctgga agaggcgcga gattacgtcc tgaaactggc acgggagcgg gaagcggagc
     9481 tactcgtgcg ctgggacacc gagtacctcg acgagcttgg cgcggacggg cctctgcgag
     9541 aagccggcgt cgaggtcgct ctctggcgtg atctgcctga cctcagggag atagccgggc
     9601 gcgcggacat cggactctct accgccacct gggccatagc cgagacgggc agcctggtgc
     9661 tggagcacgg tccggggcag ggtcgaagcg tgacgctcct gccgccgact tacgtagcga
     9721 tacttccggc ggatcgagta ttgcggacga tcccagaggc cattcggaag tatgttggcg
     9781 gcactctgcc ggcgaacgtg tgtttccaca ccgggccgag ccgttcgggg gacatcgaga
     9841 tgtcgctcgt agtagggatg cacggacccg gcgacgtgca cgtgatcctg gtcggctgaa
     9901 gggagacaag gtagaagcgg tgctaccatt gggttcatga tcgattggtc gacttgcccc
     9961 gccgttgaaa gggatcccga gcgagtcagt ggagcctggg tgttccgcgg cacgcgcgtg
    10021 ccggttgccg cgctgttcga gaacctcgag gacggcgctc aggttgacca gttcgtgcag
    10081 tggtttcccg gcgtctcgct cgaacaagcc cgcgccgtgc tcgaacacgc agcggaaagt
    10141 gcgctggctt ccgcttgagt gcgtgtactg tttgactagg gcacgcccgt tccattgcgg
    10201 gagtcgttgg gcgagcacga catctctaca gcatacgagc agggttggtc aacgctaaag
    10261 aatagtgacc tgctcgacgc agccgaaaga gaagggttcg aggttctact cactaccgat
    10321 aagaatctac ggcaccagca aaacctcggt gcgcgaagca tcgcaatcgt cgtactcaga
    10381 acgacaagct ggccgcgcat tcagcgcgaa atccctgacg tggttcgcgc catcaatagc
    10441 gccgtgccag ggagctacgt ggaagttctg tttcctatca ttagctgacc ggacctcggt
    10501 tcgaggtgtc gctcgtggtc gggatgctgg ccgtctcacg ccttttgata cagccttccg
    10561 tagagcttgg tgagggtggt ggcgcttacg ccgtggacca gcaaagaggc gcagatgacg
    10621 aggctgccaa ccacccagac ctcctctatg cctgtctcac gcagcgagaa ggcagcacga
    10681 cgaaggggag ggcgagcccg tcgttgaagc cggactcggc cgagatggcg cacctcaaag
    10741 acgagggcag gttacgctct gctactccgc cggcgactat agagctcgcg actaccgggt
    10801 aggtgggggt gatgatcgcg cctattagta cagataccca gaaatgcagc cccaggatca
    10861 ggtgcaccaa tagccccgag actatccaca tcagcggcat caagatcccc acgaggacca
    10921 cgagaagtcg ccagttgccg ctcgcgtagc ccacgggcag gcgcagggcc acgcccacaa
    10981 gggcgatgcc tagcgtgacg agggccgcct gctctaggat cagcgtctcg ttgcccagat
    11041 cggccaggtc gagcaacccg agggcggccg gcccgatcag cacccccgcg agcagggcga
    11101 tcaggggctc cgagacgggt gtgcgctcct tgagtggtcc gccgagcacg ccaagtagaa
    11161 ggagcaaacc tcccacggct gccagggata cgtacagctc gttcaacgcg tacctgcttt
    11221 ccgtcgctgt gtcctagtct gtgatcatgc tcgggcagcc gttgagcaaa acccaggtgt
    11281 gcggagcggt agagtgcgtc gatggtgcta ttccgcgctc gttcgcgtcg gctacggtcc
    11341 taccattccc ggaatggaga ccacaccctg cctttttacc actactgtcg atcgctagta
    11401 tcctcctaat cctacggggt gcttttgaag gaggatcgat cggaggctca agacgtactc
    11461 ctctgactca cggagaccgg atgccccgag tattttgagt cctgcggatt cgaacggtct
    11521 ttgggaacca ttagaagctt caaagaacac ccggctcggc cgcgatcatt atcctgtcct
    11581 tccggcacgg gtctggacgt gacatcccct cggagtctcg agatgagcct caagaaactc
    11641 ctggagagtg gagcgggtga tctgggagct ccaccctccc cagatgaagt cgaatgtgtg
    11701 gttggcccag ggcggcccga cgaggcgata gggtacgccg gcctcccgca accgctctgt
    11761 caggagctcg gactctcccg gtggcactat ctggtcgtcg cccccgtggg cgagaaaagt
    11821 cggcggatcg caggagtcga cgcgggtaac gggcgagagg agatggtagc gttcgggtac
    11881 ggtgtcccgg gtgccaccga gaaaccggtc cataccaccc aggtagccca gggaggagag
    11941 acgcgcgagg tcggtgggcg ggtagaatgc caccaccgcg gccaccccgg tatcctcgag
    12001 gctacgtgcc tcgcacccgg acgtgggacc ggagccctgc gagtaggctg taagcagggc
    12061 caggtgcccg ccagcggagc ggcccatgag agccacgcga tcggcgtcca caccgtagcg
    12121 ggcagcgttc tccttgaccc accctacagc gcaccgcacg tcggaggggg catctcgcca
    12181 actgggcggt ggcgaaagcc ggtagtcaat atcgaagacc acgtaacctc tactggcgag
    12241 ccaggcattc cagaggggga agtcgccacg ctctcccgag cgccaaccgc caccgtgtac
    12301 cacgattacc gcaggacgtc ccttggcagc gtctgccact actccaggcg gccgccagat
    12361 gtcagccttg agctcctctc cccccgggcg ggcgtaggtc acgatctccg gcgaccggtc
    12421 cgccgcgaaa gcggatcccg cgaagtaccc gaggagcgag agcgatacat cctcggcgga
    12481 ggcctccttc caagcctgcg ccaccggcac cagcgagacc gcgaccgtcg ccgcgcacag
    12541 cacggaggcg actagcgagc tttttcgggc gccgctacgg tgcgccaggg cgaccgtaaa
    12601 caaaccgaag agggcgaagg cggtgagcag gaggctcatc tccttgaaga tcatgacgat
    12661 gaagcgctgc ggatgggtcg aggacggaac gttaccgagg agttcggcga acaacggcgg
    12721 tggaaggagg ggtttcggga gcaagatcac ctgtgcccat agaagcccga gggtggccca
    12781 cgagagcacg gccgccgcac cgaaccactt cctccacgcc actcgttcgt tggttgtcat
    12841 cgtacgctac ccggcaggtt cttccttccg atggtcgtct cgcggttagg tactcttctc
    12901 aagtgcagag cgtctccttt acggttcagc agtgtcgtag aggagtggct gaagaacttc
    12961 tgcctggaca ccccttagtc cgagatcggg tcccagacga gccggctctc ctcgcactcc
    13021 gagccagaga ttgagccggt aagacctccg ctgccctatt cgggtcgagg agccaggctc
    13081 ggtccagaga aaccttaagt cgacttcccc tacaagtcaa cccgccggct cgggtagctg
    13141 attgccgagc cagggggtga gggttcgcac gtaggtcgcc gtaaggtggg ctccgttgcg
    13201 gtagacgagc acgtcgccga tgacggccgg gcatgccttc tcgaggcaca gtacaggcgt
    13261 cggatcgatc aggtatacgc cctcgacctc ctctgctacg cgggtgttga ccttcgggta
    13321 gtcgagtgcc tcgcttcgag gagacgcaca tctctgcaag tgatcgagcg agcgcgagac
    13381 gcactctggc acgttcttgt tcggatgcgg cacgtcttcg atgagcgcta ctggggcacc
    13441 ggtgccgcgc agcttcttca gggtagaggc gtatccctcc accatcgcct cgccgctagc
    13501 gtctcggggg aggcgcttgc cttcctccct cggcctgtaa gtggggaggc tgctcgtcac
    13561 gatgaggctc gggttctctt cctgcacgat gcgctctaac atctgctccc gccactcgac
    13621 gcactcgcgg tagaccctgc gcagcgacgc gttgtagatg tgcacctcgg cgggtggaca
    13681 ggcagccttg gttagcccga ccaggcgcca gttacgctcc ctggctagct cgttcaaggc
    13741 aggaaaccat tgcatggcgt gcgagtcgcc gaagagcacc actgtggtcc tggaggaggg
    13801 gttgccgtag acgcacctgg gcacttccgt ctccggaggg ttgaggtggc agccgtcggc
    13861 gtacatcagc ggccggttct ctttcgtctc ggcctctcgc ggggtgggat ggacggcttt
    13921 cgcgctcttc tgcagcgagt cgtcgtcctg cagcgccgcg gcgcccgcga cctggctctc
    13981 cggcgcctct ggaagggtcg gcgtgagggc gaagagtccc acgcccaggg cgacggacga
    14041 aacggtacac gccccgccga gggcgagggc cttgcgagga aagcgggtga gggtcttcga
    14101 gtggaggaag ggcttctcga cgaggcggtt ggtcagcacg gccgggacgt aggaggcgac
    14161 gaggacgacc agaccctctg cgggcgagag tctcccccag atcgcggcgg cgaaaacgag
    14221 cggcggccaa tgccacagat accacgagta ggagatccgc ccgacgtgcc tgaccggtcc
    14281 cagcgtgagc agccgcgccg gccctgcaga agtacctgta gactcgaacc ccgccgcgat
    14341 gatcgccgcg gtccccaggg tcggtactag cgccgcgtac ccagggaaga gggtgccgtc
    14401 gctgaagcgg aaggtcgcga acgcgatggc gccaagcccc acccacgcca gagcgaaggc
    14461 cgcccatcgg ggtagctgtc ccaggcggga ggccggcacg agcgccagca ttccaccgag
    14521 cgccagctcc caaccgcgag tcagcgtgga gaagtaggcg gccccggctt gcagttcggt
    14581 ggagtagacg ctataggcga gagaggaaat ggccacggcc gcgaaggctg ttgcaagggc
    14641 cggacgtaag cccaagccta tgcgcgagca ccaccgggcg acgcctagca ggagggccgg
    14701 ccagaccagg tagaactgct cctcgacggc cagcgtccag aagtgctgaa ccgggctcgc
    14761 ctgcaacccg gcggcgaagt agtcggtggc ccgggcggct agcagccagt tcataacgta
    14821 caggccggat gcgaccacgc ccagggagac ctcgtccatg cggaccggat cgaactgcgg
    14881 ccacatcccc accaccagga ctacggcccc gagcacgacc acagtcaacg gtagcaggcg
    14941 tttggcgcgc cgcgagtaga agcgggtgag tgagatggtg ccggtcttct ccaactccct
    15001 gacgagcagc ccggtgatca agaagcccga gatcacgaag aagacgtcca cgccgacgta
    15061 gccgccacgc gcgaacggca tcccaccgtg gtagagcagc acgactccga cggccacggc
    15121 ccttaaaccc tctacgtcag gacggaaccc ctccttcgcc ggcttgcggt tcgcttcggc
    15181 aggcgatccg tcggaggcgg cggtacgacc aaaggcgctc tggacggcgc gaaggtaggt
    15241 ggcagctccg ccgatgatgc tcaaaggagc ctcgccaaac cccgagcgtc gtgacgctct
    15301 cccggggagt aggagcacgg gctatcgacg atccagccta cgaacctctc ccgactcaaa
    15361 ctgaccaggc gtatcgtctc cggcgacatc accggcgagg ggtcgagcgc caccctcccc
    15421 acacccgcgc agggcccgta gagaatcgag acgagttctc cggctgaggt cgccctgacc
    15481 tgccagccgt cctcgtacgc gcctccgagc cacgcaaaca cctccgcctc gccctcaccg
    15541 ctaaagacca gcaatgtttg cgcgccgccg cagtccgcga tcagcgctcc cgccccgaga
    15601 cttttgccct tcgccaacag ccaccacgtc gaagccgggc gtcttaccgg cggccttcgg
    15661 ttctgttgcg tcttcttgtc ctcagtcatc cgcgctcacc gcgacggagc gttctcggac
    15721 ggcacgacca agaccgggcc gtgcgccgcc ctgagcacct tggtcgagac gctaccagat
    15781 aggacacgat cgagcgcacc cagcccgcgg ctaccgaccg cgaggagcgt tcgcccctca
    15841 tccctcttgc cggcaaccaa cgtcatggcg ggcacgggct tcgtttcggt cagcctggtc
    15901 ccgatgcgct gatgcgtgag cgccgccagt tgttcggccc gcttctcgag gtcctctcgg
    15961 cgacgtcgta aggcctcatc gagttcgcgg cgaccctggg cgctccagcc tcccaccggc
    16021 tcaggcggat gcttgtatgc ccgtaccagg acgacctcgg ccccgaagag gctagctatc
    16081 tttgcggcga ggatgtctgt cctctcggcc acttctgagc cgtcgtcgcc tactactacc
    16141 ctcgccggag gccacgcgtc ctccctgccg cgcacgatga ggaccgggca tgtagccctg
    16201 tgcaccagat cctccgaaac gctacccgta accagacgct tcacccaccc tgcccctcgg
    16261 ctgccgacga ctacgaggtc gacgttcagc tctgcggcaa gggtgtttac ctcctgcgcc
    16321 ggcctgcctt ccctgaggtg ctcccccgcg acttcccctc cctcgacccg tgccctccat
    16381 gcttgtttgc gcagtagttc tctggcttcc tcctcggcca ggtgggagga gtcgtcgaat
    16441 atcgtcgagc cgggattaga cgggggcagc acgtccggaa tgacgtggac cacatgcaac
    16501 tgcgcacctg tcttgcagga gacgtccgcc cccgctcgca tcgcgagggc agcatctcgt
    16561 gagctgtcag tggccagcaa gattttcgtc agggtagcat tcatcatcaa tcaccccttc
    16621 gcgcttttgc ctcaagcggt tcgcctaacg atcaggctgt agagatatag cgacatgacc
    16681 gcgcccagcg tggctaccag cacgctccag acgtctaagc tcgtcgttcc ggatcccccg
    16741 agaacaccca tgacgaagcc ccccagggag gccccagcca tccccaggac gacggtgacg
    16801 aagaagcgac caggatccgg gccaggcacg atccgcctgg ctaccacccc cgcgatcgcc
    16861 ccgatgatta tccagctgat gagccccatt tagctctcct ccacgttgga cccctggttt
    16921 ccgatcccgt actcctaaga gtctggggac tagagattag atcggcgttt ggcggccatt
    16981 aggattttca aaggagcggt gctccccgca ccgtggtggg gtaggattgc tccagaagat
    17041 aatgatgcgg atactgatcg tggaggacga agagcacctg gccaggcttc tcgccgaggt
    17101 cttaggcagg gaagggtacg ccgccaacac ggtctccgac ggccgctcgg gtctcacccg
    17161 agccctgagc gaggatttcg acctgctggt ggtcgactgg atgctgcccg acctcgacgg
    17221 ggtccaggtc gtgaaaagac tcaggacggc ggacgttcgc gtcccgatcc tgatgctcac
    17281 ggcccgcgcc caggtcgaag accgcgtcga gggcctggat gccggggccg acgactatct
    17341 tcccaaaccg ttcgccttcc ctgagctcct cgcgagggta agggcactct cgcgcaggcc
    17401 acccgagcct gaaacggagg ggacagtcct tacagctggc gatgtcgcgc tagatccgat
    17461 tcgccacgag gtcagacgcg ccggagagcg cgtcgacctt acggccaagg agtttgccct
    17521 gctcgccact cttatgcagc gaccgggtca agtcttcacc cgatcggtat tgcttgacac
    17581 cgtctggggc ggcaccacgg gtgcctacac caacgtggtc gacctatata ttcactacct
    17641 gcgcaagaag ctcgacgcag gcgaagaacc ttccaggatc cgaaccgtgc acggcacagg
    17701 atataccttc gacccgagac cagaataccc aggacagggc tcgtctcaag ggcaagcttc
    17761 aatagaggaa acgtgatgct agagagaatg cgttggcgcc tgacgatggg ttacgcgggc
    17821 atctttgcgc tcatcctact agttctggcc acggcggcag tggtcggctt ctcgcgtgag
    17881 ctgaccaacc agcaggacac actgctcacg caggaggcca aggacctgga gagtaatctg
    17941 ctggacggtg accgcaggga ggtcttggct gagggctcag ccgagtacag ctgggttgcg
    18001 ctcgaccccg aaggccacgt cacggacagg gatcccaccg ccgcgaccct tggaacactg
    18061 ggcctcccct ccaaagagct agccagacag tctttagaac agaacgagag ggtctccgcg
    18121 acgatccgcg gcccgaaggg tagggtgagg gtcgtgagca tgcccatgcg cgaggagtcg
    18181 ggcgaggtag tggggatcgt ccagtatgcc cgttccctca agggggtgca gcagacgatc
    18241 agcaggctcg tcctggttct gctccccctg ggactcggtg gactcggggc agccctcctc
    18301 ggcggcctct acatggccgg ccgggccatg cgtccggcgc gtgaatcctt ccagaaacag
    18361 cgtgccttcg tcgctgacgc ctcccacgag ctcaagacgc ccctcaccct gatcagggcc
    18421 gacgcagaga tggtgcttta ccgtgggcac ttgaacaaag aagaccgcaa actggtggag
    18481 cacgcgctcg tcgagactga ccgcatgagc gcgatcctct cggatctcct tctggtcgcc
    18541 cggctcgacg ccggaaaact cgaggtgtcc gaaaagccct tcgacctcac cgccgtcctc
    18601 tcggaggagg cagagcgctt cggggtgcgg gcggctgcta aagagatcca actcgaggtc
    18661 cgggtccccg gagagcttcc ggcccgtgga gatcctaaga gaaccggaca gataattggc
    18721 gtcctcctcg acaacgccgt gaggttcaca cctcctgggg ggagcatcgc cgtggccggt
    18781 aggctccacg accgctgggt ggaggccagc gtgacggaca cgggtcccgg aatctcccct
    18841 gagcacctac cccgcgtctt cgaccgcttc tacagggcgg agacctcccg cacccgcggc
    18901 agaggaggag gcatgggcct ggggctcgcc atcgcccggg ctctcgcgcg ggcccagggt
    18961 ggcgacctcg tggccgatag cgcaaaggat gggggagcga cattccgcct ccgcttgcct
    19021 cgtgaatgag cagaatggct ctggatttct gaggcttcca atcctgctaa gaggcgtccc
    19081 agatcttgag ggcttatctt ctctgtaatc gggtcatatg ccttcacgca cttttgattg
    19141 ggctcggctt cgctctgctc cggtcatatg ccgcaacctc ggtgtgggtt ctcatcccgc
    19201 cgaaagattt gaaacttcta atctgtctct gaggattctc taataccgag ggctcaagtt
    19261 tgtgcgatgg gtacgaggtt cacacgacgt cggtttctta cagctctaag cgccggtgcc
    19321 gcctacctag ccttgacgaa cacggtaggc tgcgaactgc tcgggcgcac ctcgaagctc
    19381 acggccttgc acatcccagg gtctaaccgc ttgcgggccc cgaaggtctg gccgctgcca
    19441 agcgtctcgc tcccgtctga agaggacgtc tgggccttcc gttctcgcct cgacctgagt
    19501 ccggccgcac ttgagatgat tactacgcaa gcgcacgacg acaccgcccc cggctacatc
    19561 ttcgctgctt tgaaggaagg agccggagag cacggtccaa tgataatcga cgaccaaggt
    19621 gagctcgtgt ggttcggcaa gtacaggtca gcaagggact tcaagatgca gtactacaaa
    19681 ggcagacccg tgctcacttg gtgggagggc aaggtcacgg ccgggcacgg catcggcgag
    19741 tacgtgatct tcgatggctc ttaccgcgag atagccaggg tgcgggcggg caacggttat
    19801 cgtggggact tgcacgagtt cctcatcacc cctgaggaca ccgcgctctt ggtggcctac
    19861 gctcctaggc gaacggatct ctccccgctt ggcggtccga aggatggcat ggtctgggaa
    19921 gggatcgtcc aggaggtgaa catccagagc ggaggtgtca tcttcgagtg gcacagcctg
    19981 gagcacgtcg gcatcgagga gtcctacatc gagccgtccg aacatcccga tcatctctac
    20041 gactacttcc acatcaactc catagatgta gagcccgacg gcaacctgct catctgcgcc
    20101 cgaaatacct ggaccgtcta caaggtagag cgcgagagcg gagagatcat atggcggctg
    20161 gacggcaaga agagcgactt cgagatgtgt accggtaccc agagcgcctt ccagcacgac
    20221 gccaggcgcc agagtgatgg caccatcaca atcttcgaca atggagctca ccccaaggtg
    20281 cacgatcagt cgcgagggat cgtggtagag ctcgacgagc tgaagatgag cgccaggctg
    20341 gtgcgcgagt acacctcgcc aaagaagcga ctctccacct cccagggaaa cgtgcaactc
    20401 ttgtccaact ccaacatatt gatcggctgg ggcagtgagc cgttcatctc cgagttctcc
    20461 cgtgagggcg agttactctt cgaagctcgc ttcccgcccg gcggcgagtc ctacagggcc
    20521 tttcgcttcc catggagcgg acaccccacc gacgagccag ccgttacggt agagcaagga
    20581 cccgacgaca agctcaagct ctacgccagc tggaacggag cgacagaggt cgagagctgg
    20641 gaggtgttcg ctggtccacg cctgggccga ctagggtcag tgggatctgt accccgggac
    20701 ggcttcgaga cggccatgtt ggtgcaaacc tcccattcgt acgttgctgt gcgtgctaat
    20761 gaccgtcttg gccgacctct cggcgccagc gcgcctgtga agctgtgaac acgaaagtac
    20821 tcaacagggc attcgacgga aggggttggg acgcatggcc aagggaacgg taatgtggtt
    20881 cagcgaggag aagggctacg gtttcatctc cccaaacaac gggggtgaga gtatcttcgt
    20941 ccactacact gacatagtgg gcagtggatt caggagcctc aaggtaggcg agatagtgag
    21001 ctacgtgccc gctaggggcc caagggggcg cgtagtagca cgcacagttc acagaactga
    21061 gtaggtacag ggctgtatcc tggtcgtgcc cctattcacc cgactgcgtg gaagtagcgt
    21121 tctccgaaga tccgctcacg agcgtcgcgt cgatcctttg aaaactctaa catcactcaa
    21181 aaacacctct aatgctcatg acctaaaacc gtctcaagag ctcccaagaa cccatggagc
    21241 cacgagagag gaggcgttgg gctatgacgc acccatcacg cgcagggata gggttggtga
    21301 ggctaggcga atcggacttc gtgcccgcaa aacccgagaa tgacctccgt ggcaaagacg
    21361 tctacgaccc agaaggacag cggataggtg gcgtgaagga tctctacatc gatccacagg
    21421 agcgcgaggt gcgtttcttg gagattggct tcggaggctt actggggata ggagagaagc
    21481 ccatcctggt accggtcgag gccgtgacgc ggatcacaga ggctcgagtc accatcgagc
    21541 cgggcaggac ggagaaggtg gagggacccg cccccttcga caccacagta gcgtcccctc
    21601 ggacagacgg gccgcgcgag gaccacacct cgccgcggtg ggacaacgcc gaaagaatag
    21661 ccgaccaacg acgctacatc cactcctctt tgccgtacgg cagctggcct ttctagcagg
    21721 cgttggcacc tttggaattg ctccgtagcg aagccgatcg tagtggagcc ttaggtaggg
    21781 agagctccga ggcggtgtgg ggagtcaggg gaattctttc caaagacatc ttgcccgacc
    21841 gacccgccga tcgccgcagg aggagcaggc tatgtgcgct tagggccatg gacaggctgg
    21901 ggatgcttgg cgaagagaac ctgatcccga cgctttccag aagaccgcag ctcagatggc
    21961 tggccgacga ggagggagcg aggtgggcgg tcctcgcaga gctgggtcgc atcggggagc
    22021 cggatacgtt tgaggaggct gtcgggtggg cgctggagaa ccggcccagt cccgaggaag
    22081 ccaaggccta cgtccgccga atcaggacct gttagtgact gcgcccgccg accggatgac
    22141 gcaggaagag cgtggggtag gaggatagat tcgggcgcgc gattaggtgc ggtacaatca
    22201 ctttggaccg acgatgcggc ctatactgac atacccacct ccaggccaga cagcgcttca
    22261 cctatggtga ccccggcggc gatcggggtg attctgatcc ttatcgggct cgtgttgttc
    22321 gtcgtagacc tgagcgtaac caaccatggt ctgctcaccg cggggggcat cgtgacgctc
    22381 cttgccggag gcatggtgct cctcgggacg ggcgtcccct actcgggggt gttgctcggt
    22441 gctgtcgtgg tcgtcgcgat aagcatgggg ggtgtgctct tgggg
//
LOCUS       DATMVF010000306         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_269973, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000306 DATMVF010000000
VERSION     DATMVF010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269973"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VN178_16090"
     CDS             complement(<1..331)
                     /locus_tag="VN178_16090"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWS82540.1"
                     /translation="MGVTSKSTDRNRVTLTRIAGEVGVSAMTVSNAYNHPDRLSEALR
                     DRILETAGRLGYQGPDPVGRSLRRQRTDVVGVLYSNPLSYAFDDPAAVSFLGGLSSVT
                     EEADLGLL"
     gene            420..1832
                     /locus_tag="VN178_16095"
     CDS             420..1832
                     /locus_tag="VN178_16095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWS82541.1"
                     /translation="MEVGGHAKFERKGAALVLLCMAQFVDVLDVNAVIVALHSIGQDL
                     GFSQTGLQWVVSAYVLFFAGFLLLSGRVADLWGRRRTFVAGLAVFTAASLCCGLSVSP
                     GMLVVSRAFQGLGAAITAPAALSIITTIFAEGRERDRAVAAWTAVAAGGGAAGLLLGG
                     LITDALGWEWIFFVNVPLGAAGIVLSHVLLPESRDPAASGRLDLLGAGTVTSGLVLLV
                     LGLTRIEEAGFGSPGTLATLGLAAAFFGAFVFVERHAADPLVPLGLFRLRGLVGAALV
                     AFALTAATAPVGVLVTLYLQNTLGYSASIAGLAGLPFSLCVIAGSMMGGRISERIGGR
                     VTMSSGLTVVAASALVTVGITAEGGVAYVLAGAALSGLGLGFASVASTARGTSAVEGG
                     KRGLASGLMNTSAQVGTALGLAVLLTLAATRTAALSDGAESSAEALVAGYRLAFLAAA
                     GVASLGVAATLYFFRQAKKP"
     gene            1934..2455
                     /gene="ruvC"
                     /locus_tag="VN178_16100"
     CDS             1934..2455
                     /gene="ruvC"
                     /locus_tag="VN178_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWS82542.1"
                     /translation="MISSGGRGHTILGIDPGTATMGWGVIRQEGNRLRYVQHGAITTP
                     PDWEMPRRLGRLFDGVTELVRGYGPETIAVEELFFNTNVTTAITVGQARGVALLAAYK
                     AGIEVTEYTPLQVKQAITSYGRADKRQVQEMVKALLNLPAIPRPDDAADGLAIAICHA
                     FSGSLPGKVGVGK"
     gene            2480..3070
                     /gene="ruvA"
                     /locus_tag="VN178_16105"
     CDS             2480..3070
                     /gene="ruvA"
                     /locus_tag="VN178_16105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWS82543.1"
                     /translation="MIYRLRGTLVEKDTEGVVIDVGGVGYRASASLATLRALPSLGEE
                     CVIHTRMVVREDAMLLFGFAEREERAAFDALTAVSKVGPKLALSVLSSMSPPEISETV
                     ARGDVIKFSSVPGLGKKTAERLVLELKGKSLAAFGPEPVVPGGGGGGPYVEARDALTA
                     LGYRLEEAEKALNDVPPQDSVEKYIKEALRRIGSRR"
     gene            3067..4161
                     /gene="ruvB"
                     /locus_tag="VN178_16110"
     CDS             3067..4161
                     /gene="ruvB"
                     /locus_tag="VN178_16110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWS82544.1"
                     /translation="MSTEEMDDFTVRGEGRTEELPEDITGAAGFGDFGEHPLDPEAHA
                     GDDEPTLRPGSFDEFVGQEALKQNLKIFVQAAKQRDEALDHILLDGPPGLGKTTLCRI
                     LAEEMGVGLRPTSGPTLERAGDMAAILTALEERDFLFIDEIHRLNRQIEEVLYPAMED
                     FAMDIVLGQGPSARTIRMDLPHFTLVGATTRKGLLTKPLLDRFGVAERLEYYGPEDLE
                     KIVVRNAKILGVPITEGGARQLARRSRGTPRIVNRLLKRVRDYAQVVGDGKIDEETAN
                     AALDMQGVDHLGLDRADRDYLGLVIGKFDGGPVGIGTISVALGEARDTVEDVYEPYLL
                     QSGLIQRTPRGRIATQRAYGHLGVPVPNSG"
BASE COUNT          711 a         1257 c         1450 g          765 t
ORIGIN      
        1 gcagaaggcc cagatctgct tcctctgtga ccgaactaag accgccgagg aacgagaccg
       61 cggcaggatc gtcgaaagcg taagagagcg gattcgagta cagcacccca accacgtccg
      121 tcctctggcg cctcaacgaa cgccccacgg gatccggacc ctggtagccc agcctcccgg
      181 ccgtctcgag gatccggtcc ctcaaagcct ccgagagccg atccgggtgg ttgtaggcgt
      241 tggagaccgt catcgccgat accccaacct ccccggcgat cctcgtgagg gtcacccggt
      301 tcctgtcagt actcttcgaa gtcacgccca ccccccggct tcttgcggcc cgcgaatcag
      361 atggtacact actttaacgt taaaagtata tcgttaaagt caagttgtgg aggagcatcg
      421 tggaagtagg aggccacgcc aaattcgagc gcaagggtgc ggcgctggtt cttttgtgca
      481 tggcgcaatt tgtggacgtg ttggacgtga acgcggtaat agtggccctg cattcgatcg
      541 ggcaggatct cgggttctcg cagacgggtc tgcagtgggt ggtctcggcc tacgtgttgt
      601 tctttgcggg cttcttgctc ttatcgggga gggtggcgga tctgtggggc aggaggagga
      661 ccttcgtcgc cgggttggcc gttttcacgg cggcttccct atgttgcgga ctctcggtct
      721 ccccggggat gctcgtggtc tcgcgcgcct tccaggggct tggggcggcg atcacggcac
      781 cggcggcgct ctcgatcatc accacgatct tcgccgaggg tcgagagcgt gaccgagccg
      841 tggccgcctg gacggcggtg gccgcggggg gcggggccgc gggcttgctc ctgggcggcc
      901 tcattaccga cgctttgggc tgggagtgga tcttcttcgt caacgttccc ttgggagcgg
      961 ctggaatcgt gctctcccac gtacttctac ccgagagccg ggatcctgcg gcctccggca
     1021 ggctggattt actcggggcc ggaacggtaa cctcggggtt ggtgctcctg gtcctgggac
     1081 tcacgaggat cgaagaggcg ggcttcggct caccgggtac gctggcgact cttggcctcg
     1141 ctgccgcgtt cttcggggcc ttcgtgttcg tggaacgtca tgccgccgat cccctggtgc
     1201 ccttgggact ctttcgcctg cgcgggttgg tgggggctgc cctggtcgct ttcgctctca
     1261 ccgccgccac cgctccggtc ggcgtgttgg tgaccctcta tctgcagaat acgctgggtt
     1321 attcggcgtc cattgcgggg ctggctggcc tgccgttcag cctctgcgtg atcgccggat
     1381 cgatgatggg aggccggatc tccgagcgaa tcggcggcag ggtgacgatg tcctcggggc
     1441 tcaccgtggt ggcggcgtcg gccctcgtta ccgtggggat caccgccgag ggtggtgtgg
     1501 cctacgtgct cgctggcgcc gccctttccg ggttgggctt gggcttcgct tcggtcgctt
     1561 cgaccgcccg cggaacctcg gcggtggaag gagggaagcg cggtctggcg tcgggtctga
     1621 tgaacacctc ggcgcaggtg ggtacggcgc tggggctggc cgtcctcctc accctcgctg
     1681 ctacccgtac cgccgccctc tcagatggcg cggagtcctc agccgaggcg ctggtggcgg
     1741 gctaccggtt agcgttcctg gccgccgcgg gtgtcgcatc gcttggcgtg gccgccacac
     1801 tgtacttctt tcgccaggcc aagaaacctt gatacccgag accccatgcg agcggccctt
     1861 gtagggtctg tcatccgatg cacaagatgt agaagatggt gcaagcggag gcgctagcga
     1921 gtaatcttag gccatgatct catccggtgg caggggacac accatactag gcatagaccc
     1981 tgggacggcg acgatggggt ggggtgtgat ccggcaggag ggaaaccggt tgcgctacgt
     2041 gcagcacggt gccataacca caccgcctga ctgggagatg ccccgcaggc tcgggcggct
     2101 gttcgacggc gtgaccgagc tggtacgcgg ttacgggccc gagaccatcg ctgtcgagga
     2161 gctcttcttc aacaccaacg tgactacggc gatcacggtc gggcaggcga ggggtgtcgc
     2221 gttgcttgcg gcttacaagg ccgggatcga ggtgaccgag tacacgccct tgcaggtcaa
     2281 acaggcgatc acatcttacg gaagggcgga caagcgacag gtgcaggaga tggtgaaggc
     2341 gctgctgaac cttccggcca tacctcggcc cgacgacgcg gccgacggcc tggccatagc
     2401 catctgccac gctttctccg gcagcctgcc aggcaaggtc ggggtgggga aatagaggaa
     2461 cttgcgcgat aattagccca tgatctacag gttgcgcggt actctggtcg agaaggatac
     2521 ggaaggtgta gtaatcgatg ttgggggcgt cggctatagg gcctcggcct cgctggcgac
     2581 gttgcgggcg ttaccctcgt taggcgagga gtgcgttatt cacaccagga tggtggtgcg
     2641 tgaggacgcg atgcttcttt ttgggttcgc ggagcgtgag gagcgggccg cgttcgacgc
     2701 cctgacggcg gtgagcaagg tcgggccgaa gctggccctt tccgtactgt cttcgatgtc
     2761 gcccccggag atctccgaga cggtggccag gggggatgtg atcaagttct cctccgtacc
     2821 ggggcttggg aagaagacgg cggaaaggct cgtactggag ctcaaaggca agagcctcgc
     2881 cgccttcggc cccgagccgg tcgtcccagg cggcggtggg ggtggtcctt acgtcgaggc
     2941 gcgggatgca ctaacggcac tcgggtacag gctcgaggag gccgagaagg ccctcaacga
     3001 cgtgccaccg caggattccg tggagaagta cataaaggag gcgttacggc ggatagggag
     3061 caggcgttga gcacagaaga gatggacgac tttaccgtgc ggggcgaggg tagaaccgag
     3121 gaattgcccg aggacatcac gggggctgcg ggattcggag acttcggtga gcatccgctc
     3181 gaccccgagg cccacgccgg ggacgacgag cccacgctga ggccgggctc gttcgacgag
     3241 ttcgtcgggc aggaggcgct caagcagaac ctcaagatct tcgtgcaggc tgccaagcag
     3301 cgcgacgagg ccctggacca catactgctc gacggccctc ccgggcttgg caagaccacg
     3361 ctgtgccgca tcctcgccga agagatgggg gtcgggctca ggccgacgag cggacccact
     3421 ttggagcgag ccggagatat ggcggcgatc ctgaccgcgc tggaagaacg tgacttcctt
     3481 ttcatagacg agatccaccg tctcaacagg cagatcgaag aggtcctcta ccccgcgatg
     3541 gaggacttcg cgatggacat cgtcttgggc caggggccgt cggcccgcac catcagaatg
     3601 gacctgccac atttcacgct ggtcggtgcg accaccagga agggcctgct cacaaagcct
     3661 ctgctcgacc gttttggagt cgccgagagg ctcgaatact acgggcccga ggacctggag
     3721 aagatcgtgg tccgcaacgc gaagatactc ggagttccga tcaccgaggg tggcgccagg
     3781 cagctcgccc gacgcagccg cgggacgccc cgcatcgtca accggctcct caagcgggtg
     3841 cgcgactacg cccaagtcgt cggcgacggg aagatcgacg aggagacggc gaacgcggcg
     3901 ctcgacatgc agggcgtcga ccacctcggc ctcgacaggg cagaccggga ttacctcggg
     3961 ctcgtcatcg gcaaattcga cggggggccg gtcggcatcg gtacgatctc cgtggccctc
     4021 ggcgaggcgc gcgataccgt cgaggatgta tacgagccct acctgctgca gagcgggctg
     4081 atccaacgca ccccccgagg ccggatcgca acgcaaaggg cctacgggca cctcggcgtt
     4141 cccgtcccta acagcggata atccctggct ggcggaagcg gaa
//
LOCUS       DATMVF010000307         8026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_89528, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000307 DATMVF010000000
VERSION     DATMVF010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89528"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VN178_16115"
     CDS             <1..1320
                     /locus_tag="VN178_16115"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HWS82545.1"
                     /translation="MTFVLWALLLSIQLRNPLLNGSESPMLRMLFFWGMLLPLGAYWS
                     VDRARSALPRPSPGFLSLATFGLFLQIAFVYWFTAALKSGPEWRSDYTALYYALSLDQ
                     LATPIGHLLLDFPSLLQALTLSTVVLEALGPLLLFCPFFTGPVRTGAALAFMGFHLGI
                     WLTMDIGIFPWISAFCMVCFFPTWFWDRVSTLRSVVLRRVELARRLRLATARLGDALV
                     AFSKAVVSFLMEARQLLFAGPVQPLAAGGVGTSETRHDEAFSEEPAELRSSLAANLLA
                     FFFIFYILCWNLTTVTSFTLPERTVPLGPFLGLDQYWGMFAPSPSNEDGWYVIPGKLR
                     DGQKVDLMPITRDDYSMHPISYEKPQDIPATYKNEHWRKYLENIYNRDHASQRLYFGQ
                     YICRQWNAHHRGADTLETLRIIYMLELTLPDYRQSKPEKVELWNHTC"
     gene            1471..2778
                     /locus_tag="VN178_16120"
     CDS             1471..2778
                     /locus_tag="VN178_16120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWS82546.1"
                     /translation="MMANRWDKIALASVLAGVIVRIIWGLVIHPPFDHLYSDMGAYVE
                     RAQHLAVGAGLQRTDAFFPPGTHALLAAPMTHFGTERAGLWGGAVLWCVLSAAIPFFA
                     WRLARLLLTPPAAALTALFCAFWPLYITYGGYFTSETPSLAFLLASLWAGYRAARLSG
                     REAGWLGLAAGLLGGVAVANRPQLILNLAVLVVPLLFRLRRQALALTGIVAGSALILA
                     GTVLHNSAAAGKPTGLSENAGLNFWIGHCDVHDVTTVDPARNITFQFANPVWMQLGRG
                     GTYYFEGRLVWDQGFFYEMGLRCIERNGLGHVRVLARNVLNMGATTVPWPQVNETSQR
                     DVVRLSNLAYSLLLPLVVIDSIFVLLRRPASGRSSGEAVMLLQLACALVVAIFFFGDP
                     RVRSSYDVFGLALLAARITDRFGLGESRRKPADSGLRKDQYPS"
     gene            3128..5227
                     /locus_tag="VN178_16125"
     CDS             3128..5227
                     /locus_tag="VN178_16125"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HWS82547.1"
                     /translation="MRLIISEKANTAKKIAQFLSEGSVREGKHRSVPHHTFTWKGEET
                     VSVGLKGHVLNPEYPEEYSNWQKVEPRELIDAEIKKSVSEKGVAQAVTSLSKKADRVV
                     IATDFDREGELIGVEALSLVFEANPKLIDHVERARFSALTPGEVGRAFDELMEVSNDL
                     ADAGEARQDIDLIWGATLTRWVSRATKRYGSAFLSVGRVQSPTLVLIAERERERRAFI
                     PVPYWELEVELKNGEPFTVRHQTERFQEEARAKSAYENVAGTATVEEVEQKSATRKPP
                     PPFNTTAFLTAAANIGISPSRAARIAEDLYTDGFISYPRTDNTVYPGSLDLREVLGYL
                     SSVEGVGKHAERLLSHEKLTPTRGKKETTDHPPIYPTGCASKESMRDDRWKIYQLVVR
                     RFLATLSAPAKTLRTTLRFDSGGEPLVTGGTVVTEEGWLGVYPYSRRADEEIPNLSEG
                     DVVQVIGKEIHSKETQPPGRYGAGRLINVMEDLGLGTKATRPNIIQNLYDRGYVHDDP
                     LVPTEKGMAVAQALTDFASEIASHEMTAELERSMDEISEGKVSKDSVVDSSRDVLRRV
                     YDHLQSSETEFADIVWEGIRTDETVGKCPESGHDLIIRRNRKSRKRFVGCTGYPDCRV
                     TYPLPQRGEIIPLGTQCDACGSPEIKVLGGKRPWVTCINMDCPKKQEQREAAAKAKEG
                     GEDSSTPSAQELQKSTT"
     gene            5394..6158
                     /locus_tag="VN178_16130"
     CDS             5394..6158
                     /locus_tag="VN178_16130"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWS82548.1"
                     /translation="MPPIIGITATLKEDIDEVAERPLGRFVRADLDYVEGVAGAGGAP
                     VVLPPAGDERAAEAVIRSLDGLLLSGGSDLDPGYYGEEPVPELGVTLPERDAFEMALV
                     GLALRRGMPVFGICRGMQVLNVALGGTLYQDLPSQWGRDLLGHRQDTPKWQPTHEVRV
                     EHGSYIAEVMERESVKVNSYHHQGIKDLAEGLVAKGFSSDGVVEAVEAGDLSERWLLG
                     VQWHAEAMRGAGPQQESLFEAHVSAAERHALRRAAA"
     gene            6053..6823
                     /gene="tmk"
                     /locus_tag="VN178_16135"
     CDS             6053..6823
                     /gene="tmk"
                     /locus_tag="VN178_16135"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWS82549.1"
                     /translation="MARRGYARSRSSAGEPLRGARLSGGASRLAQGRCLAGRPAAQST
                     RRGIFVTIEGPDFSGKSTLVRAIRPLLAGSTPATHFTREPGGTPVAEIIRGVVLDPGI
                     QMDAWTEAYLYAAARAEHVRHAILPRLEAGENLLCERYLDSSLAYQGFGRGLGVEAVR
                     AMNAYAVGTVVPDRTFYLRLGHDERERRARELGMPPDRIEAVGAGFMRRVEEGFEELA
                     RVEPERIEVLDASLSAEDLARRVVGRMRELQYTRGADG"
     gene            6816..7979
                     /locus_tag="VN178_16140"
     CDS             6816..7979
                     /locus_tag="VN178_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HWS82550.1"
                     /translation="MADRQDTFEGVLGNETALRLLGRALQTGEVSQAYLFHGSPGVGK
                     RMVALRFGAALVAGGEAGAEDRALRGLHPDLTEIVPQGTFTTIGQVREVVRLAASRPF
                     EGARRVFILGADTLNVQAANALLKTLEEPEGETVFVLLANSREGVLSTILSRAQVLRF
                     NPVPTGVVAEFLRERGVAEPGLAAELGRGSVGLSLRYAEDPEFGRLREAVFRAGFAFS
                     EDFEERHEAVREIVGRAESMGEAREREFVADFEEPGRRAKEGAKRAGKAARDGAVRES
                     LELLALLYRDAAVIESGAEELAANTDRVGEIRGYVEEYAGADWAGAARALEEARAALA
                     YNVSPEATLEVTLSRIRRKILAPSRGSLTSGSRAGASPGSATRGTLTSGSATR"
BASE COUNT         1447 a         2507 c         2635 g         1437 t
ORIGIN      
        1 atgaccttcg tcttgtgggc gttgctgctc tcgatacagt tgcgcaaccc cctgctcaac
       61 ggctccgaga gccccatgtt gcgcatgttg ttcttctggg gcatgttgtt accgctaggc
      121 gcctattggt cggtggatcg cgcgcggtct gcgctcccgc gcccttctcc aggctttctg
      181 tcgctggcca ccttcggcct gttcttgcag atcgccttcg tctactggtt caccgccgcc
      241 ctgaagtcgg gacccgagtg gcgctcggat tacaccgcgc tctactacgc gctcagcctg
      301 gaccagctcg ccaccccgat cggccatcta ctccttgact tccccagttt gctccaggcg
      361 ctgacactca gcacggtcgt gctcgaggcc ctcggtccgc tcctgctgtt ctgccctttc
      421 ttcaccggtc cggtccgcac cggggccgcg ctggccttta tgggcttcca cctcggcata
      481 tggcttacga tggacatagg gatcttcccc tggatcagcg cgttctgcat ggtctgcttt
      541 ttccccacct ggttctggga cagggtttcg acgctccgct ccgtggtgtt gcggcgggtc
      601 gagctcgccc gccgcctgcg actcgcgacg gcgcggttgg gcgacgctct cgtcgccttc
      661 tccaaggcgg tggtgtcgtt cctcatggaa gcccgccaac tcctgttcgc gggtccggtc
      721 cagcccctgg cagccggggg ggtgggcacg tccgaaacgc ggcacgacga ggccttctcc
      781 gaagaaccgg cggagctgcg gtcctcgctc gctgcgaacc tgctcgcctt cttcttcatc
      841 ttctacatcc tctgctggaa cctgaccacg gtcacctcgt tcacgttgcc cgagcgcacc
      901 gtcccactcg gacccttcct cggcctcgac cagtactggg gcatgttcgc cccctccccg
      961 agcaacgaag atggctggta cgtgatccca ggcaagctgc gcgacggcca gaaagtggac
     1021 ctcatgccta tcacccgcga cgattactcg atgcacccca tctcttacga gaaaccgcaa
     1081 gacatccccg ctacctacaa gaatgagcac tggcgtaagt acctggagaa catctacaat
     1141 cgggatcacg ccagccaacg actgtacttc ggccagtaca tctgccgcca gtggaacgcc
     1201 catcataggg gcgcggacac cctcgaaacg ctccggatca tctacatgct ggagctgaca
     1261 ctgcctgact accgccagtc caaaccggag aaggtggagc tctggaacca cacatgctag
     1321 cgacgcgtgt gcccgagccg tgtccacctg cacgccgcat ccgcagctga tgggcgccga
     1381 accgctggca ggtaccggtc tcgatcgccg caacgctgcc ccgaaagccg gcgggtctct
     1441 ggcgaggcgg cgcacggcgc ctttcgggaa atgatggcga accggtggga caagatcgcc
     1501 ctggcctccg ttctggccgg cgttatcgtc cgcataatct gggggctggt gatccaccct
     1561 cccttcgatc acctctactc ggacatgggt gcgtacgtcg agcgggccca gcacctcgct
     1621 gtcggcgccg gcctgcagcg aaccgacgcc ttctttccgc cggggaccca cgcactgctg
     1681 gccgccccca tgacgcactt cgggacggaa cgggcgggat tgtggggagg ggccgtgctg
     1741 tggtgcgtac tctccgctgc gatcccgttc ttcgcctggc ggctggcacg cttgttgctc
     1801 accccacccg ccgccgcact gacggcgctc ttctgcgcgt tctggccgct gtacataacc
     1861 tatggggggt acttcacctc agagacgccc tcactggcgt ttctgctcgc gtccctgtgg
     1921 gccggctacc gggccgcccg gctctcgggc agggaggcgg gctggctcgg cctggcggcc
     1981 ggcctgctcg ggggcgtagc ggtcgccaac cgcccgcaac tcatcctgaa cctcgcggtg
     2041 ctggtggtgc ctttgttgtt tcgcctccgc cgccaggccc tggccctcac cggcatcgtc
     2101 gccggcagcg ccctcatcct ggccggcacc gtattgcaca acagcgcggc ggccggcaaa
     2161 cccaccggcc tcagcgagaa cgcgggcttg aacttctgga tcggacactg cgacgtccac
     2221 gacgtgacca ccgtcgatcc cgcaaggaac atcaccttcc aattcgccaa tcccgtctgg
     2281 atgcaactgg gtcggggcgg gacgtattac ttcgagggac gcctggtatg ggatcaaggg
     2341 ttcttctacg agatgggact gcggtgtatc gaaagaaacg gtctcggtca cgttcgggtc
     2401 ctggccagaa acgtcctgaa catgggcgcc acgacggttc cctggcctca ggtcaacgag
     2461 acctctcagc gggacgtggt acggctcagc aacctggcgt acagcctcct gctgccgctg
     2521 gtcgtcatcg actccatctt cgtcctcctc aggcgtcccg catccggtcg gtcctcgggg
     2581 gaggcggtga tgctgctcca gctggcctgc gcgttggtgg tggcgatctt cttcttcggt
     2641 gaccctcgtg tacgcagctc ctacgatgtc ttcggccttg ccctgctcgc cgcccgcatc
     2701 accgaccggt tcggcctcgg ggagtcaagg agaaagcccg ccgatagcgg gctcaggaaa
     2761 gatcagtatc cttcctgaac ccgcacattt cagcgagtca gcacgcatcg gccttcggcc
     2821 tccgcttgtc agccggtcag gaacaggctg aaatgctgac cagcgcgcga gggggaaggg
     2881 tcacaaccct tccccctcgc gccaaaagcc aattgaaata ctggcaacag tggtttatac
     2941 cccaggggga tgtcctccgc gacgatagga ttatcggagg tctgcgcacg atcgatggga
     3001 tgggaaggta tgctgcagcg ttacagtacg gtaggggtaa ggtctttcga gaacagggcc
     3061 gccccgaata acaaggactt gctatcttcg gacaagatgc tctttgccgg tgtaaaatgg
     3121 taacaagatg cgactgatta tctctgagaa agccaatact gcgaagaaga tcgcccagtt
     3181 cctctctgaa gggtctgtca gggaaggcaa gcaccgctcc gtgccgcacc acacgttcac
     3241 ctggaagggg gaagagacgg tctcggtagg cctcaagggg cacgttctca acccggagta
     3301 tcccgaggaa tactcgaact ggcagaaagt cgagccgcgg gagctcatag acgcggagat
     3361 caaaaagtcg gtctccgaga agggcgttgc ccaggccgta acgtcgctct cgaagaaagc
     3421 ggacagggtg gtcatcgcca cggacttcga ccgtgagggg gagttgatcg gtgtcgaagc
     3481 cctctccctg gtcttcgagg ccaacccgaa gctcattgac cacgtagagc gtgcccgctt
     3541 ctccgcactc acgcccgggg aggtcggccg cgccttcgac gagctcatgg aggtctccaa
     3601 cgatctggcc gacgccgggg aggcgcggca ggacatagac ctgatctggg gcgcgaccct
     3661 tacccgctgg gtctcgcgcg ccacaaagcg ctacgggagc gccttcctct cggtaggacg
     3721 cgtccagtca ccgacgctcg tcctcatagc cgagcgtgag cgcgagaggc gtgccttcat
     3781 ccccgtgccg tactgggagc ttgaggtgga gctgaaaaac ggcgagccgt tcaccgtccg
     3841 ccaccagacc gagcgcttcc aggaagaggc gagggcgaag agcgcctacg agaacgtcgc
     3901 cggcaccgca acggtcgagg aggtcgagca gaaatccgcg acccgcaagc cgccgccgcc
     3961 tttcaacacg acggccttcc tgaccgcggc cgcgaacatc gggatcagcc cttccagggc
     4021 ggcccgaata gcagaagacc tgtacacgga tggcttcatc tcatatccca ggacggacaa
     4081 caccgtctac ccaggctcgc tcgatctgcg tgaggtgctc gggtacctga gtagcgtcga
     4141 gggggtcgga aagcacgccg agaggttgct ctctcatgag aaactcacac ccacgcgcgg
     4201 aaagaaagaa acgaccgacc accccccgat ctacccgacc ggctgcgcgt cgaaggagtc
     4261 gatgcgggac gaccggtgga agatctacca gctcgtcgta aggcgctttc ttgccaccct
     4321 ctcagccccg gcgaagacgc tgcgcacgac gctgcgcttc gactctggcg gcgaaccgct
     4381 cgtcacggga gggacggtcg tcacggagga gggttggctc ggcgtctacc catacagcag
     4441 gcgcgcggac gaagagatcc ccaacctctc cgagggcgac gtcgtgcagg tcatcggaaa
     4501 agagatccac tccaaagaga cccagcctcc cggccgctac ggcgcgggtc gcctcataaa
     4561 cgtgatggaa gacctcggac tcggcacgaa ggcgacgcgc ccgaacatca tccagaacct
     4621 ctacgaccgc ggctacgtcc atgacgaccc gctggtccct accgagaagg gcatggccgt
     4681 ggcgcaggcc ctcacggact tcgcgtccga gatcgcctcc cacgagatga ccgccgagct
     4741 ggagcggagc atggatgaga tctcagaggg caaggtcagc aaagactcgg tcgtcgactc
     4801 ctcgcgcgac gtgcttcgca gggtctacga ccatctccag agctccgaga ccgagttcgc
     4861 ggacatcgtg tgggagggca taaggaccga cgaaaccgtg ggcaagtgcc ccgagagcgg
     4921 gcacgacctg atcatccgcc gcaaccgtaa gagccgcaag cgctttgtcg gctgcacagg
     4981 ctaccccgac tgccgggtaa cctatccgct acctcagcgg ggtgagatca tacccctcgg
     5041 tacccaatgc gacgcctgcg gctctccgga gataaaggtg cttggcggga agcgtccctg
     5101 ggtaacatgt ataaatatgg actgccccaa gaaacaagag cagcgtgagg ccgccgcaaa
     5161 ggcgaaagag gggggtgagg actcatcgac cccgagcgcc caggaacttc agaagtccac
     5221 cacatagaga acaggctcga attagacctg gccgatgaac ctcctttcta tggaccctcc
     5281 gcgtctatcc gcgcgactgg ttacctgatc ctcctcgcca tcgtcgtcgt agtctggtat
     5341 tctgcagtat atagttaagt taagtgaaga tagtgttaac aggtgagtag agtttgcccc
     5401 caatcatagg cataacggcg acgttgaaag aggatatcga tgaggttgcg gagcgtccgc
     5461 tgggcaggtt cgtaagggcc gacctggatt acgtcgaggg cgtcgctggg gccgggggcg
     5521 cgcccgtggt gttgccgcct gcaggggatg agagggctgc agaggcggtg atccggagcc
     5581 tcgacgggtt gctcttgagt ggtggaagcg acctcgaccc cggctattac ggcgaggagc
     5641 cggtacccga gcttggggta accttacccg agcgggatgc tttcgagatg gcgttggtag
     5701 ggttggccct gcggcgtggg atgccggtct tcgggatctg ccgggggatg caggtgttga
     5761 acgtggcact tggcgggacg ctttaccagg accttccttc gcagtggggg agggacttgc
     5821 tcggtcacag gcaggacacc cccaagtggc agcccacgca cgaggtaagg gtcgagcacg
     5881 ggtcctacat cgccgaggtc atggaacgcg agtcggtaaa ggtcaactct tatcaccatc
     5941 aggggatcaa ggacctcgcc gaagggctgg tggccaaggg cttctcttcg gatggcgtgg
     6001 tcgaggcggt cgaggccggg gatctctccg agcgttggtt gctcggtgtc cagtggcacg
     6061 ccgaggctat gcgcggagcc ggtcctcagc aggagagcct cttcgaggcg cacgtctcag
     6121 cggcggagcg tcacgccttg cgcagggccg ctgcctagcg ggaaggccgg ccgcgcagag
     6181 tacacgccgg ggcatcttcg taacgatcga aggcccggat ttctcgggca agtccaccct
     6241 ggtgcgggcc ataagaccac tcctcgcagg ttcgacgcct gccacacact tcacccgcga
     6301 gcctggcggt acgccggtgg ccgagatcat caggggggtc gtcctcgatc ctgggattca
     6361 gatggacgcc tggacggagg cgtatttgta tgcggctgcc cgcgccgagc acgtgcgcca
     6421 cgcaatactc ccgcgactgg aagccggaga gaacttgctc tgcgagcgct acctggactc
     6481 tagcctcgcc taccagggct tcgggagagg gctcggcgtc gaagcggtac gcgctatgaa
     6541 cgcatacgcc gtcgggaccg tcgtacccga caggactttc tatctgcggc tcggccacga
     6601 cgagagggaa cggcgggcca gggaactcgg gatgcctcca gacaggatcg aggcggtagg
     6661 cgcgggcttc atgcggcgtg ttgaggaggg tttcgaggag ttggcgcgtg tggagcccga
     6721 gaggatcgag gttctcgacg cctcgctctc ggcggaggac ctcgccagac gagtggtggg
     6781 taggatgcgc gagttgcaat atactcgtgg ggctgatggc tgaccggcag gataccttcg
     6841 agggcgtgct cggaaacgag acggcgctgc ggctactagg gcgggcgcta cagaccggcg
     6901 aagtctcgca ggcttacctg ttccatggtt ctcccggtgt gggcaagcgg atggtggcgc
     6961 tgaggttcgg ggccgcgctg gtcgccggag gggaggcggg ggcggaagac agggcgctca
     7021 ggggactgca tcccgacctg acggagatag taccccaggg gacgttcacg actatcgggc
     7081 aggtgaggga ggttgtgcgc ctggcggcga gccggccgtt cgagggtgcg cgccgggtat
     7141 tcatccttgg ggccgatacg ctgaacgtgc aggccgcgaa cgcgctactc aaaacgctcg
     7201 aagaacccga aggcgagacg gtattcgtgc tgctcgcgaa ctcgcgtgag ggggtactgt
     7261 ccacgatcct ctcccgcgct caggtcctgc ggttcaaccc cgtgccgacg ggcgtcgtcg
     7321 cagagttcct gcgagagagg ggtgttgccg agcccggact cgctgcggag ctcggtaggg
     7381 ggagcgtcgg gctctccctg cggtacgcgg aggaccccga gttcgggagg ctgcgcgagg
     7441 cggtcttcag agccgggttc gccttctcgg aggacttcga ggagcggcat gaggccgtca
     7501 gggagatcgt cggccgcgcc gaatccatgg gcgaggcgcg ggaacgcgag tttgtggccg
     7561 atttcgaaga gccaggcagg cgcgcgaagg aaggagccaa acgcgcggga aaggcggcga
     7621 gggacggtgc ggtgagggaa tctctcgaat tgttggccct tctgtacaga gatgcagcgg
     7681 taatagagtc gggtgcggaa gagctggccg ccaacacgga cagggtggga gagataaggg
     7741 ggtacgtgga ggagtatgct ggcgcggatt gggccggggc cgcccgtgcc ctggaggaag
     7801 cacgcgcggc acttgcgtat aatgtctctc cggaggcgac cctggaggtg acgttatcgc
     7861 ggatacggcg gaagatactc gcaccctccc gaggctcgtt gacgtcaggg tcccgggctg
     7921 gagcatcacc aggctctgct acgcgcggga ccttgacctc agggtcggct acacggtagt
     7981 cgtcgcgacc gaggtggggg agttgatcgg tcgcgtcgcc ctgacg
//
LOCUS       DATMVF010000308         8430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_41933, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000308 DATMVF010000000
VERSION     DATMVF010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41933"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VN178_16145"
     CDS             complement(<1..786)
                     /locus_tag="VN178_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWS82551.1"
                     /translation="MPDSLLDLTAYELAKEIEAGEVSAREAAEVANTRVDEVEDGVNA
                     FVTPTPELALERAEAVDAKDGDRALWEGVPLAVKDVLSTRGVRTTCGSRILENFEPLY
                     DASALLNFGEDLIMVGKANCDEFAMGSSTENSAWGPSRNPWDLDRVPGGSSGGSAAAV
                     AAGESWWALGTDTGGSVRQPAALCGIVGLKPTYGAISRYGMIAFASSLDQCGPLTRDV
                     TDAALLLRLMEGRDPHDSTSVGIEGGVEMPSRENLSGLRFGVPR"
     gene            complement(788..1069)
                     /gene="gatC"
                     /locus_tag="VN178_16150"
     CDS             complement(788..1069)
                     /gene="gatC"
                     /locus_tag="VN178_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWS82552.1"
                     /translation="MISEEQVRHVANLARLGLTDDEIEKMGGQLDAILDSIEKIQELD
                     LSGVPPTANPLDLSNVLRPDEPRPEFSQEQALSPAPERVDDLFAVPRID"
     gene            1199..3118
                     /locus_tag="VN178_16155"
     CDS             1199..3118
                     /locus_tag="VN178_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWS82553.1"
                     /translation="MARGDRNAAAEPRPGGGSQEMEIHEAASEFDRTGPRGYQPIENY
                     GIIGDLHTTALVGMDGSIDWLCLPHHDSPSVFAAILDDAIGGRFKISPVGGEATTKQL
                     YWPDTNVLVTRFFTPDGVGEVTDYMPIGTSAEGRHRIIRRVEVVRGAMTFRMECTPAF
                     DYARQEHETRIVPGGVTFASSDLSLGLASLIPLQQEGDGSCAEFTLQEEQSTVFVLRE
                     IEAGEDCGVCLSTTEEEEIFMQTVEYWRRWLSRCTYTGRWREMVRRSALILKLLTFEP
                     TGAIVAAPTMGLPEGIGGERNWDYRYTWIRDAAFTLYGLLRIGFTEEAKGFINWLGSR
                     CQGSNPDGSLQLMYGIDGRQDLREEILDHLDGYRGSRPVRLGNGAYDQLQLDIYGELM
                     DAVYLYNKHGSPISYDLWTHLRTLINWVCDNWQNEDEGIWETRGGRRNFVYSRLMCWV
                     AIDRGLRLADKRSFPAERDKWLKVRDEIYEEIMEKGWSPEREAFVQSYGDDTLDASNL
                     IMPLVFFLSPSDPRMLKTLDAINRPPKDGGLVSNSLVYRYDVKKSADGLTGEEGTFSL
                     CTFWLVEALTRAGRLEESRLIFEHMLGYANHLGLYAEEIGHHGEALGNFPQAFTHLTL
                     ISAAYNLDRALGSRG"
     gene            3144..4553
                     /locus_tag="VN178_16160"
     CDS             3144..4553
                     /locus_tag="VN178_16160"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HWS82554.1"
                     /translation="MAAKHYDAVVIGAGQAGVPLSQALAGSGRSTALIEREHVGGTCI
                     NVGCTPTKTMVASAKTAYIDRRSAEYGVHNGPVSVDMREVRGRKRAMVDSFRASNLRR
                     IEAAEGLDLISGEASFTGPRTLAVRTNEGEALELVAENVFINAGARPANPPIEGLDEV
                     PVLNSTSVMELGELPEHLLVLGGSYVGLEFAQMFRRFGSEVTVVQRSGQLMGREDADI
                     AEAVAEILREDGIEILLGTQTHRAEQDGAGKIVLTVDTSEGERTISGSHLLVAAGRPP
                     NTETLNLGAAGIETDKRGFIVANERLETSAPGVYALGDVKGGPAFTHISYDDFRIIRT
                     NLLEGGDATITNRQVPYTMFIDPQLGRVGLTEQEARDQGRDVLVAKIPMSYVSRAVEL
                     GETRGLMKAVVDAESAQILGCSVLGIEGGEIMAMIQIAMLGEMPYTALRDAVFAHPTL
                     AESLNTLFSSVEYPQRAAV"
     gene            complement(4624..5469)
                     /gene="folD"
                     /locus_tag="VN178_16165"
     CDS             complement(4624..5469)
                     /gene="folD"
                     /locus_tag="VN178_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HWS82555.1"
                     /translation="MAKILDGKAAAAEVRSEVAEEVAGIREYGVPVRLDVILAGDDPA
                     SITYVASKERDSEEVGIESKVHRFSTDVSQGELSDLVERLNEDSAVSGFFMQLPLPEG
                     LDSMPLISSISPAKDVDGLSPESVGRLVLGLPSLLPCTPHGVIQLLKRNGIELSGSEA
                     VVVGRSNLVGKPLAQLLLRENATVTLCHSRTRDLPEVTRRADILVVAAGKREMVGAEH
                     VGEGAVVVDVGIHRKEEGGLVGDVRFDEVEPKAAWISPVPGGVGPMTRAMLLHNTVTA
                     AQMGL"
     gene            complement(5477..7102)
                     /locus_tag="VN178_16170"
     CDS             complement(5477..7102)
                     /locus_tag="VN178_16170"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011447.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HWS82556.1"
                     /translation="MPHVYVTGHKNPDTDTIASAIGYAEYKNLVDPENHYEAARLGEV
                     NAQTAWALKRSGAQQPQLLEHIMLRVRDVMAGDVAVARKNDPLRNVGLTMAQRNVSQL
                     PIVEDDGSLTGIITERNLARMYVRESRGASTFAESPVSVGAMLEVLEGELLVGEEERE
                     SSGQLWVISMSVASMGQSMEPGDIVVIGDRPKGQRRAIELGAGVLVVSNGVRPGDEVL
                     ELARERGTAVLLSPLDSYVTSRLVQLSVPCWEVMSENPLTVRPDDLVTDITEQVMEVH
                     YRAAIAVDEKNVPIGVVTRTDLLNPNPRRVLLVDHAEIGQSVAGVEKAEVVEILDHHH
                     IGDIETTSPIPATFDPVGSTATLIMERFKASGLKPEESTAKMLLAALLSDTVILNSPT
                     TTDRDREVAGYLEEMLDLDANEFGTEMFEASSDVSSLSAQEIVGRDAKEYGTSSGNKV
                     SVSQVEIVGTGLLERKDELIDALEDLQGENEYLFSALMVTDIIEGGTQLLCVGDCTPV
                     HQAFDCQAQNGVIDLPGVMSRKKQVAPLLLSVL"
     gene            complement(7312..>8430)
                     /locus_tag="VN178_16175"
     CDS             complement(7312..>8430)
                     /locus_tag="VN178_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWS82557.1"
                     /translation="PRGIAVFYRTNAQSRALEDVLVREGIPYQVVGGVRFYERAEIKD
                     AMAYLGVISNPTDAGALERIINVPKRRLGSTSVARLQDHARRNGVSLYEALAEPDAVD
                     LTGAAKKACRAVRDLFEGWRVAAQEVPPAGLIGAVLAESGYRAELEAENTVEAESRLE
                     NLEELINAAREYERVGPEPTLEGFLQEQALYSDQDSLTSEGGRVTLMTLHNAKGLEFG
                     HVFVVGMEEGTFPHARSLDEHNLEEERRLAYVGITRARETLTLTHAKLRSSWGEREYR
                     MPSRFLSEIPEQYKSGTMPGLSNASGRGGWGVALSGRGGVERAASGGRAFKTGERVRH
                     TKFGVGEVVEAGVGKVVIRFGTEERTFVPEIAPLSRIT"
BASE COUNT         1524 a         2779 c         2632 g         1495 t
ORIGIN      
        1 gcgaggaacg ccgaagcgca gaccgctgag attctcgcgc gagggcatct ccacacctcc
       61 ctcgatgccc acggaggtcg agtcgtgcgg atcgcgcccc tccatcagcc gcagcaggag
      121 ggccgcatcc gtgacgtcgc gggtcagcgg cccgcactgg tccagcgagg aggcgaaggc
      181 gatcatcccg tagcgcgaga tcgccccgta ggtgggcttg agcccgacga tgccgcagag
      241 cgccgctggc tggcgcacgg agccgcccgt gtccgtcccg agcgcccacc agctctcgcc
      301 cgccgcgacg gccgcggcag atccgcccga ggacccgccg ggtacgcggt ccaggtccca
      361 ggggttgcga ctgggacccc aggccgagtt ctccgtcgat gagcccatcg cgaactcgtc
      421 gcagttggcc ttgccgacca tgatgaggtc ctcgccgaaa ttcaggagcg ccgaggcgtc
      481 gtagaggggt tcgaagttct ccaggatcct cgacccgcac gtcgtcctca cgccacgcgt
      541 agagagcacg tccttgacgg cgagcgggac cccctcccag agggcgcggt ctccgtcttt
      601 ggcatcgacg gcctcggccc gttcgagcgc cagctcaggg gttggcgtca cgaaggcgtt
      661 gacgccgtct tctacctcgt cgacacgcgt gttggcgacc tcggcggcct cgcgggccga
      721 gacctcgcca gcttctatct cctttgcgag ctcgtaggcg gtgagatcca gcaggctatc
      781 cggcatctca gtcgatcctg gggacggcga acagatcgtc gacacgctcg ggggccgggg
      841 agagcgcctg ttcctgcgag aactcggggc gcggctcgtc ggggcgcagg acgttcgaga
      901 ggtcgagcgg gttcgccgta ggcggcacgc ctgagaggtc cagctcctgg atcttctcta
      961 tgctgtccag gatagcgtcg agctgcccgc ccatcttctc tatctcatcg tctgtgagtc
     1021 ccaaccgcgc cagattggct acgtggcgca cctgctcttc ggatatcatg tccggtctcc
     1081 ggtccatcgc ttgtagaacg agaacttatc ttaacgcagc gtccgtagtc aatgaaggag
     1141 gtaagaggat agcccgtcag aggctctgta tcggtaagag gcctcatgag gtagcctgat
     1201 ggcaagaggc gacaggaacg cggccgccga accccgtccc ggaggaggaa gccaggagat
     1261 ggagatccac gaggcagcga gcgaattcga taggaccggt ccaaggggct accaacctat
     1321 cgagaactac gggatcatcg gcgacctgca caccacggcc ctggtgggga tggacggttc
     1381 catagactgg ctgtgcctgc cgcaccacga ctccccaagc gtcttcgcgg ccatcctcga
     1441 cgacgcgata ggcggaaggt tcaagatctc acctgtaggg ggcgaggcca ccaccaaaca
     1501 gctctattgg cccgacacca acgtgctcgt cacgcgcttc ttcacgcccg acggggtcgg
     1561 tgaggtcaca gactacatgc cgatcggtac atccgcggag ggtcgacacc ggatcatccg
     1621 tcgcgtcgag gtggtacgcg gcgccatgac cttccggatg gagtgtactc cggccttcga
     1681 ttacgcccgc caggagcatg aaaccaggat cgtccccggc ggggtgacct tcgcctcctc
     1741 cgatctcagc ctgggactgg cgtcgctcat ccccttacag caagagggcg acgggtcctg
     1801 cgccgagttc acgcttcaag aggaacagtc cacggtattc gtgctcaggg agatcgaagc
     1861 cggagaagac tgcggggtat gtctctccac tacggaggaa gaagagatct tcatgcagac
     1921 cgtcgagtac tggcgccgct ggctctccag gtgcacctac acgggccgct ggcgcgagat
     1981 ggtccgccgc tcggccctga tcctgaagtt gctaactttc gagcctacag gcgcgatagt
     2041 ggcggcaccc accatgggtc tgcccgaagg catcggcgga gagcgcaatt gggattaccg
     2101 ctacacctgg ataagggacg cagcattcac cctatacggg ctcctgcgca tcgggttcac
     2161 agaagaagca aaggggttca taaactggct aggatcgcgc tgccagggat ccaaccccga
     2221 cgggtcgttg caactcatgt acgggataga cggacgccag gacctgagag aggagatcct
     2281 ggatcacctc gacggctacc gcggctcacg tcccgtcagg ctcgggaacg gcgcctacga
     2341 ccagctgcaa ctcgatatct acggcgagct catggacgcc gtctatctct acaacaagca
     2401 cggctccccc atctcctacg acctgtggac ccatctcagg accctgatca actgggtctg
     2461 cgacaactgg cagaacgagg acgaagggat ctgggagacc aggggcggcc gcaggaactt
     2521 cgtgtactcc aggctcatgt gctgggtcgc catcgacagg ggtttgaggc tcgccgacaa
     2581 gcgctcgttc cccgccgagc gcgacaagtg gctgaaggtc cgggacgaga tctacgagga
     2641 gatcatggag aagggttgga gtccggagcg ggaggctttc gtgcagtcct acggcgacga
     2701 caccctggac gcctcgaacc tgatcatgcc cctcgtcttc ttcctttcgc ccagcgatcc
     2761 caggatgctc aagacgctcg atgccatcaa ccgccccccg aaagacgggg gcctcgtctc
     2821 caatagcctc gtctaccgct acgacgtgaa gaaatccgct gacggcctga cgggcgagga
     2881 gggcaccttc agcctctgca ccttctggct cgtcgaggcg ctcacgcggg cgggccggct
     2941 cgaggaatct cgcctcatct tcgagcacat gctcggctac gcgaaccacc tcgggctcta
     3001 cgccgaggag atcgggcacc acggcgaagc gctgggcaac ttcccgcagg ctttcacgca
     3061 cctcaccctc atcagcgcgg cctacaacct cgatagggcg ctcggcagca ggggttagca
     3121 ccgttaggaa ggagtctccc tctatggcag caaagcatta cgacgccgtg gtgatcgggg
     3181 cgggccaggc gggggtcccg ctctcccagg cgctcgccgg gtcggggcgt agcacggccc
     3241 tgatcgaacg cgagcacgta ggcggcacct gcatcaacgt gggctgtact cccaccaaga
     3301 cgatggtcgc cagcgcgaag acggcctaca tcgaccggcg ttcggccgaa tacggcgtcc
     3361 acaacggccc ggtctccgta gacatgcgcg aggtgcgagg ccgaaagcgg gcgatggtcg
     3421 acagcttcag agccagtaac ctgagacgca tcgaagccgc agaggggctc gacctgatct
     3481 ccggcgaagc cagctttacg ggcccgagaa cgctcgcggt gcgcacgaac gagggcgaag
     3541 ccctcgaact cgtcgcggag aacgtgttta tcaacgccgg ggccaggccg gccaaccccc
     3601 cgatcgaggg tctggacgaa gtgcctgtcc tgaactcgac ctccgtcatg gagctcggcg
     3661 agttgccgga gcacctccta gtgctgggcg gcagctacgt gggactggag ttcgcccaga
     3721 tgttcaggcg tttcggcagc gaggtcacgg tcgtgcaacg aagcggacag ctgatgggcc
     3781 gcgaggacgc cgacatagcc gaagcggtcg ccgagatcct gcgcgaggac ggtatcgaga
     3841 ttctgctggg aacccagacc caccgcgccg agcaggacgg cgccggaaag atcgttctga
     3901 cggtagacac gtccgagggg gagcgcacga tttcgggttc tcacctcctc gtggcggccg
     3961 gaaggccgcc caacaccgag accctgaacc tcggcgcggc cggcatagag acagacaaac
     4021 gcggctttat cgtggccaac gagcgcctgg agaccagcgc gcccggcgtc tacgcactcg
     4081 gagacgtgaa ggggggaccg gccttcaccc acatctccta cgacgacttc cgcatcatcc
     4141 gaaccaattt gctcgaaggc ggcgacgcca caatcacgaa tcgccaggtc ccctacacca
     4201 tgttcataga tccacaactg ggccgggtcg gattgaccga gcaggaagcc agggaccagg
     4261 gccgcgacgt gctcgtagcg aagatcccga tgtcctacgt ttcgcgggcc gtagaattgg
     4321 gcgagacgcg cggactaatg aaggccgtcg tggacgccga gagcgcccag attctgggct
     4381 gctcggtgct cggtatagaa ggaggggaga tcatggcgat gatccagata gccatgttgg
     4441 gcgagatgcc gtacacggcc ctccgcgacg ccgtcttcgc ccaccccacg ctcgccgaat
     4501 cgctgaacac cctcttctcg agcgtagagt accctcaaag agccgcggtg tagagtcggg
     4561 cgttcggtcc cccgcagagg cctgtagggg cgggcttcaa acccgcccga acgtgaaccg
     4621 cccctacagg cccatctgtg cggccgtgac cgtgttgtgg agcagcatcg cccgggtcat
     4681 cgggccgaca ccccctggga cgggggagat ccaggccgcc tttggctcca cctcatcgaa
     4741 gcgcacgtca ccgacgagcc ccccttcctc tttgcggtgt atgccgacat cgacgacgac
     4801 cgcgccctcc ccgacgtgct cggccccgac catctcccgc ttgccggcgg cgacgacgag
     4861 gatgtcagcc ctccgggtga cctcgggaag gtcccgcgta cgcgagtggc agagcgtgac
     4921 ggtcgcgttc tcgcgcagga gtagctgagc gagcggcttg ccgacgaggt tcgaacgccc
     4981 cacgacgaca gcctcgcttc ctgagagctc tatgccgtta cgtttgagga gttggatcac
     5041 gccgtgcgga gtgcacggca gaaggctcgg gaggccgagg acgagcctcc ctacgctctc
     5101 ggggctgagg ccgtccacgt ccttcgccgg actaatgctc gagatcaggg gcatagagtc
     5161 gagtccttcg ggcaaaggga gttgcataaa gaagcccgaa acggccgaat cctcgttcaa
     5221 ccgctccacg aggtccgaaa gctctccctg ggagacgtcg gtggagaagc ggtggacctt
     5281 cgactctatc ccgacttcct cgctgtcacg ctccttggag gccacgtagg tgatggaggc
     5341 ggggtcatcg ccggccagaa tcacatcgag ccgcacgggc acgccgtatt ctctgatccc
     5401 tgcgacctcc tcggcaacct ccgagcggac ctcggccgcc gccgctttgc cgtccagtat
     5461 cttagccacg cgtctcctaa aggaccgaga gcagcaaggg ggccacctgc tttttgcgac
     5521 tcatcacgcc cggcaggtct ataacgccgt tctgggcttg gcagtcgaat gcctggtgca
     5581 cgggggtgca gtccccgacg cacaggagtt gggtgccgcc ctctatgatg tccgtgacca
     5641 taagcgcaga gaacaggtac tcgttctcac cttgcaaatc ctccagggcg tcgataagct
     5701 cgtctttgcg ctccaggagt ccggtaccga cgatctccac ctgggagacg ctcaccttgt
     5761 tacccgaact cgtcccgtac tccttcgcgt cgcggccgac gatctcctgt gcggagagcg
     5821 aagagacgtc cgaggaggct tcgaacatct ccgtaccgaa ctcgttggcg tcgaggtcga
     5881 gcatctcctc gagatacccg gcgacctcgc ggtcgcggtc cgtggtggtc ggggagttca
     5941 ggatcaccgt gtccgagagg agggcggcaa gcaacatctt tgcggtcgac tcctcgggtt
     6001 tcagaccgct cgctttgaag cgttccatga tcagggtggc cgtcgagccg acggggtcga
     6061 aggtggcagg gatgggagag gtcgtctcga tgtccccgat gtgatggtgg tccaggatct
     6121 cgacgacctc ggccttctcg acgcccgcta cgctctgccc gatctcggcg tggtcgacga
     6181 ggagcacgcg tctcggattg gggttgagga gatctgtcct cgtgaccacc cctatgggca
     6241 cgttcttctc gtccacggcg atcgccgccc tgtagtggac ctccatcacc tgctcggtta
     6301 tgtccgttac gaggtcatcg ggtctgacgg tcaagggatt ctcgctcatg acctcccagc
     6361 aggggacgga gagctgtacc aggcggctcg tcacgtaaga gtcgagggga gagagcagta
     6421 cggccgtccc cctctccctg gccagctcca ggacctcatc cccgggacgc accccgttgg
     6481 agacgacgag taccccggcg ccgagctcta ttgccctccg ctggcccttc gggcggtccc
     6541 cgatcaccac gatgtccccg ggctccatag attggcccat cgaggccacg ctcatcgaga
     6601 tcacccacaa ctggcccgag gattcacgct cttcctcccc gaccagaagc tcgccctcga
     6661 ggacttcgag catcgctccc accgagacgg ggctctcggc gaaagtggaa gcaccgcgcg
     6721 actcccgcac gtacatgcgc gccaggttcc gctccgtgat gatcccggtg agagagccgt
     6781 cgtcttccac tatcggcagc tggcttacgt tccgctgggc catagtaagg ccgacgtttc
     6841 gcaacgggtc gttcttgcgg gcgacggcca cgtccccggc catgacgtcc cttacgcgca
     6901 gcatgatgtg ctccagcagc tgaggctgtt gtgccccact cctcttcaag gcccacgcgg
     6961 tctgtgcgtt tacctcgccg agcctggccg cctcatagtg gttctcggga tcgacgaggt
     7021 tcttgtactc ggcgtacccg atagccgagg cgatggtatc cgtatcaggg ttcttgtgac
     7081 ccgttacata gacgtgtggc attgtactcc ttttgcccct tacctaagcc tcatcgtggc
     7141 acataggatc cggttcgatt cctacccgat acctgcccac gagaagtcgg tcctcagttg
     7201 tactccctag agggtaccag aatctgtatt tgtcgatacg agaagacccc agccacgaag
     7261 aggagggcca acgtgcccta taccccctgg ctcacagtgt ctggcccttc cttacgtgat
     7321 cctgctcaac ggggctatct cgggtacgaa ggtcctctcc tcggtcccga acctgatgac
     7381 gaccttcccg acgccggcct ccacgacctc cccgaccccg aatttcgtgt gcctgaccct
     7441 ttcaccagtc ttgaatgctc tccctcctga agctgctctc tctacacctc cccgcccgga
     7501 cagagcgacg ccccatcctc cgcggcccga ggcgtttgaa agacctggca tcgttcctga
     7561 cttgtactgc tccgggatct ccgaaaggaa acgagatggc atcctgtact cgcgctcgcc
     7621 ccagctggag cgcagcttgg cgtgggtgag ggtgagcgtc tccctggcgc gtgtgatgcc
     7681 gacgtaggct agtctccgct cctcttcgag attgtgttcg tcgagcgaac gggcgtgtgg
     7741 gaaggtcccc tcctccatcc cgacgacgaa gacgtgcccg aactcgagcc ccttcgcgtt
     7801 gtggagcgtc atcagcgtca cgcgaccgcc ttccgaggtc agagagtcct ggtcagaata
     7861 aagggcctgc tcctgcaaga agccttcgag cgtcggctcg ggcccgacgc gttcgtactc
     7921 cctggcggcg tttatgagct cctcgaggtt ctccaggcgc gactcggcct cgacggtgtt
     7981 ctcggcctcg agctccgcgc ggtagccgga ctcggcgagg acggccccga tcagccccgc
     8041 cggaggaacc tcctgcgcgg cgacccgcca gccttcgaag aggtcgcgca cagcccggca
     8101 cgccttcttc gcggcccccg tgaggtccac tgcgtctggc tccgcgaggg cctcgtagag
     8161 cgagacgccg ttccggcggg cgtgatcctg cagtctcgct acggaggtcg aaccgaggcg
     8221 ccttttgggg acgttgatga tgcgctctaa agcgccggcg tcagtcgggt tggagatcac
     8281 cccgaggtac gccatggcgt ccttaatctc cgcccgctcg tagaagcgaa cgcccccgac
     8341 gacctggtac ggaatcccct cgcgcaccag cacgtcctct aatgcccggc tctgggcgtt
     8401 tgtccggtag aagactgcta tgcccctcgg
//
LOCUS       DATMVF010000309         4950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_42140, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000309 DATMVF010000000
VERSION     DATMVF010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42140"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /gene="mutS"
                     /locus_tag="VN178_16180"
     CDS             <1..1724
                     /gene="mutS"
                     /locus_tag="VN178_16180"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWS82558.1"
                     /translation="YGLDGHPALAGAAGALIQYLASLRGGGPPEQVVTFRPYDPGVGM
                     VLDAATRRNLGLDDLVETVDRTRTPMGRRALRRWLERPLLEVDRIRQRLGAVDVLAGD
                     YMLREDVREHLGRIPDVERIATRIVRLSASPNDLLSLKGALEEIGPLRETLGPASSVS
                     SLLSRVLGAMEEPPGVRELVAAAISEESGEIIRPGYSGELDEAREFRDGAHEWLTRFE
                     AQERLKTGLKTLKVGYRDGEGYFIEVAGKETSAVPERYQHRKALKHNARYVTVELKEH
                     ESRMLGARDEVERIERGILAEIRAAVKEAAPELQKVSRAIAVVDVISSFAAAATELRY
                     CRPELVEGRGISISGGRHPVVEHNLEMPFVPNDSQVDGDSRLQIITGPNMAGKSVYLR
                     QVALIALLAQTGSYVPADEASLGVVDRIFTRVGAEDRLASGESTFMVEMTEAASILNS
                     ATERSLVILDEVGRGTSTYDGMSLAWAIAEYLHDDVQALTLFATHYHELTRLTEMLPG
                     CRNYKANVEEVGGEIVFLHRIEPGAESSSYGVHVAKLAGLPPQVTDRAGEILTRLEAE
                     GVKDFAG"
     gene            1731..2318
                     /gene="mutL"
                     /locus_tag="VN178_16185"
     CDS             1731..2318
                     /gene="mutL"
                     /locus_tag="VN178_16185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWS82559.1"
                     /translation="MKLFPTLVSEEAAHYAGRVRVLSPEVAHRIAAGEVIERPASAVK
                     ELIENSLDAGASRVEVEIQNGGIAMIRVSDDGSGMSPEDAGRSVTEHATSKIRTADDL
                     ASVVSLGFRGEALHAIGAVSDLTLTTRERGKEDPLGARVRVFAGKIVESATAAHSPGT
                     TVEVRDLFLNLPVRRGFLGTVRAEARATYDNGITL"
     gene            2758..3096
                     /locus_tag="VN178_16190"
     CDS             2758..3096
                     /locus_tag="VN178_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82560.1"
                     /translation="MDQGEQITGTRDEHYNLVSVLYHTLNAADTCDRYALDAETAGDE
                     RLAAFFRKTQALQTGVAEEAKQHLGILEPPPEFGAGSTSGGSSTTAYGNTPDMPRSPL
                     NEPPHPGGLR"
     gene            complement(3161..3610)
                     /locus_tag="VN178_16195"
     CDS             complement(3161..3610)
                     /locus_tag="VN178_16195"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWS82561.1"
                     /translation="MSTEENKALVRRFVDEVQSRGNIDALDEICTPEFVNHSAPPGVP
                     SNREGVKQLTAMFRQAFPDSYFTIEDMMAEGEKVGTRKTFHGTHQGEFMGIPPTGQQV
                     SMGLIDIVRIADGRVVEHWSMADNLGMMQQLGIVPLPGDTEEASPTY"
     gene            complement(3780..>4950)
                     /locus_tag="VN178_16200"
     CDS             complement(3780..>4950)
                     /locus_tag="VN178_16200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWS82562.1"
                     /translation="IELEENPEAEVLLGRFGAKPSETPVTVWQGQDVLKNPTNLEFAR
                     TTGLEVDAPLERTYDLVVVGAGPAGLGASVYGASEGLSTLALESVALGGQAGTSSRIE
                     NYPGFPAGLSGFELASRVLVQADKFGARTAVPREAVGLRRENGHYYIELSEGGEVAAR
                     SVIVATGARYRRLDVPRLGRFEGVSVHYAATEAEAQRCEGEEVAVVGGGNSAGQAALF
                     LAGRTRRVYLLIRGSDLGKSMSKYLVDRITAAKNVELLTSTEVREFMGEDRLEGAVVE
                     DNRSGIRRNLRIRALFAFIGADANTSWLEGAVELDERGFVLTGRELNGSALDEDAWRE
                     RSREPFLQETSLPGVFAAGDARSGSIKRCTSAVGEGAMAVALVHQYLADASVQRA"
BASE COUNT          888 a         1616 c         1586 g          860 t
ORIGIN      
        1 gatacgggct ggacgggcat ccggcgctcg ccggcgcggc aggcgctttg atccagtacc
       61 tggcctccct gcgcggcggc ggtccgccgg agcaggtggt gaccttccgt ccttacgatc
      121 ctggtgtggg gatggtcctc gacgcggcga cgaggcgcaa cctgggcctg gacgacctgg
      181 tcgagaccgt agaccggacc cgcacgccga tgggccggcg cgcgctcagg cgctggctgg
      241 agcgtcctct gctggaggtg gaccgtatca ggcaacgtct cggggccgtc gacgtgctcg
      301 ccggagacta catgttgcgc gaagatgtgc gtgagcatct gggacgcatc cccgatgtgg
      361 aacgcatcgc cacgcgcatc gtaaggcttt cggcctcgcc caatgatcta ctctcgctca
      421 agggcgcgct ggaggagata gggcccctca gggagacgtt ggggccggca tcttctgtgt
      481 cctcgctgct ctcgcgggtg ctgggggcga tggaggagcc gccaggggta agggagctgg
      541 tcgccgcggc gatctccgag gagagcggtg agatcatccg tcccggctac tcgggtgagc
      601 tcgacgaggc gcgcgagttc agggacggcg cccacgaatg gttgacgcgc ttcgaggccc
      661 aggagcgtct gaagacgggc ctgaagaccc tgaaggttgg ttacagggac ggagaaggct
      721 actttataga ggtggcgggc aaggagacga gcgctgtccc cgaacgctac caacaccgca
      781 aggcgctcaa gcacaacgcc cgctacgtta cggtggagct aaaggagcac gaatcgcgga
      841 tgctcggggc ccgcgacgag gtcgagcgca tcgagcgcgg catcctcgcc gagatacgcg
      901 ccgccgtcaa ggaggcggct ccggaactgc agaaggtctc gcgcgccata gccgtcgtgg
      961 atgtgatctc ctccttcgcc gccgcggcga ccgagctccg ctactgccgc cccgagctcg
     1021 tagaagggcg gggcatcagc atctccggtg ggaggcatcc cgtcgtcgag cacaacctgg
     1081 agatgccttt cgtccccaac gactcccagg tcgacgggga ctcgcggctc cagatcatca
     1141 cgggtcccaa tatggccggc aagtccgtct acctcaggca ggtggccctc atcgccctgc
     1201 tggcccagac cggctcctac gtccccgccg acgaggcatc ccttggcgtc gtcgaccgta
     1261 tcttcacgcg ggtcggggcc gaggacaggc tggcgagcgg tgagtctacc ttcatggtgg
     1321 agatgaccga agcggcgagt attctgaaca gcgcgacgga gcggtcgctg gtcatcctgg
     1381 acgaggtagg acgcggcacc tcgacctacg acgggatgag cctcgcctgg gccatcgccg
     1441 agtacctgca cgacgacgtg caggccctca cgctcttcgc cacccactac cacgagctta
     1501 cgcgtctcac ggagatgttg cccggctgtc gaaactacaa ggccaacgtc gaagaggtgg
     1561 ggggtgagat cgtattcctg cacaggatcg aacccggagc agagtcctcc tcctacggcg
     1621 tccacgtggc gaagctcgcc ggattaccgc cccaggtcac cgaccgggca ggcgagatcc
     1681 tcacccgcct cgaggccgaa ggcgtcaaag actttgccgg gtgagaccgg atgaagctct
     1741 ttcctacgct ggtctccgaa gaggccgccc actatgccgg acgggtccgg gtgctgtcgc
     1801 ccgaggtggc gcacaggatc gccgccgggg aggtcatcga gcgtccggcg tccgccgtca
     1861 aggagttgat cgagaactct ctcgacgcgg gggccagcag ggtcgaggtg gagatccaga
     1921 acggcggcat tgccatgatc cgggtcagcg acgacggctc ggggatgtca cccgaggacg
     1981 cgggccgctc ggtcacggag cacgcgacga gcaagatccg gacggcggac gacctggcca
     2041 gcgtcgtctc tttgggcttc cgcggcgagg ccctgcacgc catcggcgcg gtctcggatc
     2101 tcacgctcac cacccgcgaa cgcggtaagg aagatcccct cggcgcccgc gtgcgggtct
     2161 tcgccggaaa gatcgtcgag tcggcgacgg cggcccattc tcctggcacg acggtggagg
     2221 tgcgcgacct gttcctgaac cttcccgtac gccgcggttt cctcggcacg gtgcgggccg
     2281 aggcacgcgc aacttacgac aacgggatta ccctttagcg tccgtctcga tcccgacggt
     2341 gcgacaaccg ctcaactcat cgtataggtg cgaggcccgc gaggaggcgg gttctgccgc
     2401 cttcaggtgg acccacgcaa gctggggcaa cgcctcgcgc ggccttcctt gcgacagtat
     2461 ggccggaggg cagggtcgag actgccgtcg tcctctagcc gcgcaggttt ctgctacctc
     2521 tgctcttctt cggaccgcga tccgagcgca gccacaaaga gacgtgagcc ggttgcagag
     2581 tctctcgtat cgcgctcgtc acacatcgac atcgcctcct tcttgcgctg aggaagcttc
     2641 cagcacctac taacaagtct ctacaacgag cgcggggaca gccaaacgtg tgtttcgaac
     2701 actttagtcg agggtacgcc cagaacacag aggcgcaacc gaaagaagga gaccacagtg
     2761 gatcagggcg agcagatcac tggaaccaga gacgagcact acaatttagt gagcgtcctc
     2821 taccataccc ttaacgctgc tgatacgtgc gaccgttacg ccctcgatgc cgagaccgcc
     2881 ggcgacgagc gcttggctgc gttcttcaga aagacgcagg ctttgcagac cggcgttgcc
     2941 gaggaagcca agcagcacct gggcatcctc gagccaccac cggagttcgg ggccggctcc
     3001 acttcgggtg ggtcgagtac gaccgcctac ggtaacacgc cggatatgcc acgctcaccg
     3061 ctaaatgagc cgccccaccc gggagggctt aggtagcagc ggcgcgatgt cccggcgata
     3121 gacttccgag aaacaccttc tacgagcttg ggaaaataga ctaataggtc gggctggctt
     3181 cctccgtatc tcccggcaga gggacgatgc caagctgctg catcatgccc aggttgtctg
     3241 ccatagacca gtgctccacg accctcccat cagcgatacg cacgatatcg atgagcccca
     3301 tagacacctg ttggccggtt ggggggatgc ccatgaactc gccctgatga gtaccgtgaa
     3361 aggtcttacg cgtcccgacc ttctcccctt cggccatcat gtcctctatg gtgaaatacg
     3421 aatccgggaa ggcttgacga aacatggccg tgagctgctt gacgccctca cggttcgaag
     3481 gcactcccgg cggggcgcta tggtttacga actcgggggt gcaaatctca tccaaggcgt
     3541 cgatgttgcc tctgctctgc acttcgtcga cgaagcggcg cactagggcc ttgttctcct
     3601 cggtggacac agcgtcttcc ccttcctcgt gggcgggtga gcttgtggca acatgatgcc
     3661 acgggcagac acctagcgcg aaccacggag aaagcatctt ccacgcacac cggtgaatag
     3721 agggtacagg cgatggggcg cttctagtac cgttcttgca gcggtgcggg acggaatcct
     3781 caggctctct gcacgctggc atcggccaag tactgatgga ccaacgctac cgccatagca
     3841 ccctctccca ccgcggaggt acaccgcttg atcgagccac tacgcgcgtc gccagctgcg
     3901 aatacgcccg gcaggctcgt ctcctggagg aatggctccc gcgagcgttc tcgccaagcg
     3961 tcctcatcga gcgcagaacc gttcagctcc ctaccggtca ggacgaagcc gcgttcgtcg
     4021 agttcgacgg caccttccag ccagctggtg ttggcatcag ccccgatgaa ggcaaagagc
     4081 gcccggattc tgaggttccg tcgtataccc gagcggttat cctcgactac ggccccctct
     4141 aggcggtcct ctcccatgaa ttctcggacc tccgtgctgg taagtagctc gacgttcttg
     4201 gcggccgtga tccggtcgac gagatacttc gacatgcttt tgcccaggtc gcttcccctt
     4261 ataagcaggt aaacccttct cgtgcgtccc gccaggaaca gtgccgcctg gcccgccgag
     4321 ttgcctccgc ctacgaccgc gacctcctcg ccctcgcacc tctgtgcctc ggcctccgta
     4381 gctgcgtagt ggacgctcac gccctcgaaa cgcccgaggc ggggcacgtc gagcctgcgg
     4441 tagcgagccc cggttgcgac tatcacgctg cgggccgcga cctcaccgcc ttcggagagc
     4501 tctatatagt agtgaccgtt ttccctcctc agaccgaccg cctcccgggg tacggcggtg
     4561 cgggcgccga acttgtcggc ttggaccagg acgcggctgg ccagctcgaa gccggagaga
     4621 cctgcgggga agcccgggta gttctctatg cgcgatgagg tgccagcctg ccccccaagg
     4681 gccaccgact ctagggcgag ggtggagagg ccctcagaag ctccgtagac cgaagcgccg
     4741 aggcctgcgg gtcctgcccc caccacgacg aggtcgtagg ttcgttctaa gggagcgtcg
     4801 acctcgaggc ccgtggtgcg ggcgaactcg aggttcgtcg ggttcttcag cacgtcctgg
     4861 ccctgccaga cggtcaccgg cgtctccgag ggcttcgcac cgaacctgcc gagcaagact
     4921 tcggcctcgg ggttctcctc gagctcgatc
//
LOCUS       DATMVF010000310        17595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_272037, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000310 DATMVF010000000
VERSION     DATMVF010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 17595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17595
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272037"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VN178_16205"
     CDS             complement(<1..412)
                     /locus_tag="VN178_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82563.1"
                     /translation="MKDHDPDSPSVIQAIEENGAEFLLALGRAAGAEERDDGRVRWTI
                     GNSPIDYHNCVVQADLTREEADGEIEASLERMRAHGVPGSWHVGPSMRPSDLGERLIA
                     HGFEYGGDDIGMAVDLSTLPEEVPTPADFVVERVR"
     gene            complement(543..2624)
                     /locus_tag="VN178_16210"
     CDS             complement(543..2624)
                     /locus_tag="VN178_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82564.1"
                     /translation="MGKRLRRYLAAFGIGVLALGGVPAAGFGQAGDYQNLEPGKRADL
                     DEKASVNVVFVGYDQDDVSAGRFRSTLPDQYKPIVRSRYFYNKSVKKSLLGLNYTYDY
                     NVRFANGAYERRFFSYLSDAAKPAPLTDFQKLYNGEPRKFCKPPNGEPKPCQKQGVRD
                     IKKNYRISALSVEEWLAANPPAGIDTKRNTVFFINWWGDGEKPRKGFKHHVYTKTNEP
                     EPDASFNFGRDDSRKLIAWGGTTADDEESGLGSTRRIWFHDLSAGPDSFTDNWNVDDP
                     DLTGDGKEDYRLPPVWEYYAKDGYRPASRLTGDLSKVTRYVAIDLFFTSSPIYPPQFT
                     PRLLPNKINLDVNTVEGIPGKNYSRRYQTPSLLLDEIRELHRLPYSVDQQDVAYKGKA
                     KRCYLLWRAHVPCYLKKFPAYTGTANLFLYGANNLSRLLDDERTAREYEAALLNWAVK
                     DGPKGLTSPPVLGLADDNHRDGTQSFAFSFISPDIAKNYGLTTTEIHEYGHHLNLSHP
                     FDGFDYENQTDFGWDGKYYFTGVGNEVNSIMSYVDLNWDFSQFDRDNTDRFQAAAYIN
                     NANALARKIQNDPDADQAAAELAAADVAYGEAKAALARHNYAGTFEKAREAYEHALQG
                     ARDAGVPVKASDNGRTASNKAKVSSVIPGRYIDRPRPAEREKPMKRQDAADIEVHKAQ
                     HFSPLAHRLRR"
     gene            complement(2813..3865)
                     /locus_tag="VN178_16215"
     CDS             complement(2813..3865)
                     /locus_tag="VN178_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HWS82565.1"
                     /translation="MALNEALLKRLIETPGAPGREERQREIAREELGSLTDEVRTDAL
                     GSVIGTKKGSGNGRVMIAAHTDEIGFLVKHIDDKGFVRLQTLGGHDPANMVSQRVLVT
                     TADGETLRGALQPARKPPHLVTRGEEQKPPKADEFYVDLGMDAETVKEKVRIGDYAVM
                     DRTLEKVGETYVGKAMDDRVGVFVMYEALRAVGDHQATIFAAATSQEEVGLRGAEASG
                     SALEPTVVVALDVTLAMDVPGGGNENTITELGKGVAIKIMDGYSISHPKLVEHLRAIA
                     EREDIPYQMEILPAGGTDAGGVQRLHGGIPAVTLSIPCRYVHTPNEMVSAKDVEAAIT
                     LLARYLEEASDADYAL"
     gene            complement(3979..4764)
                     /locus_tag="VN178_16220"
     CDS             complement(3979..4764)
                     /locus_tag="VN178_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWS82566.1"
                     /translation="MAETREHQVEEATDAGNPETPSWFRSKAVAVHLYTASGAVLALL
                     MLVAAFQGEAVKALWLMLASLLIDSTDGLLARRFRVSEALPFFDGALLDNIVDYMTYV
                     FAPVVLLWSEGYLPAGNAGIVLAALPLLASSYQFCRVDAKTEDHYFLGFPSYFNVVAF
                     YAIVFHPGPLVLAAVLVACSFLVFVPIRYVYPTRTVAFRKLSLTLTALWLASYATILW
                     QMPDPDPPILAFSILYLVYYFGLSLYLSGKLLEAQRLEEETAD"
     gene            complement(4804..6501)
                     /locus_tag="VN178_16225"
     CDS             complement(4804..6501)
                     /locus_tag="VN178_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HWS82567.1"
                     /translation="MQRLRSTLDRIDRKGYGAYKDLSGSHDFSSFVLFVDRVQRDPFA
                     PPSLIRIRTTANSFEPALFENLVRRVAFEDFLTRSVEREIRRTVRGNRGSGGSGRVEI
                     QRTTQVVLPRTSMVVEPNYIEARMAVGLPARGRSVDARAAKAVLLEELPEVVRRALVP
                     AAEGGVDVERAMLHVEAVEDADHLRGLLPGLGLVAFVADGAVLPRESGASDRPLRDGA
                     VPFGSPEEYRVEVELPNGGFVSGMGVPEGVTLVAGGGFHGKSTLLSALSWGVYDHVPG
                     DGRELVVARRDAVKVRAEDGRSVSGVDISAMIGDLPGGRSTKDFSTPNASGSTSQAAN
                     IAEAIEVGTSLLLVDEDTSATNFMIRDERMRELVRREPISPFIDLVRPLHRSLGVSTV
                     VVVGGVGDYLDVADRVILLEDYAPSDATPRSREVTKRFPPRAPLADREVLPPQERAID
                     ASSIDLRRGKRRSVRGRGLHTIELGRERVDLSYLEQLAEAGQTEAVARIIGEWAATGE
                     VSEVGELVRDALASVSEKGLDSLGGHGGHPGEMSLPRAQEVAAATNRIRPLRAVPKM"
     gene            complement(6511..6750)
                     /locus_tag="VN178_16230"
     CDS             complement(6511..6750)
                     /locus_tag="VN178_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWS82568.1"
                     /translation="MGAQYVIDENGERVSVILPIEEYERLIEELEELEDVLATQAYDE
                     AKAELERGEDEVIPWEQAKKEIEEERTRRGHQDAV"
     gene            complement(6796..7788)
                     /gene="trpS"
                     /locus_tag="VN178_16235"
     CDS             complement(6796..7788)
                     /gene="trpS"
                     /locus_tag="VN178_16235"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWS82569.1"
                     /translation="MQSKERVFSGIQPSGTSHLGTYLGALKNFVALQDDYETFYCIVD
                     LHALTTPRDPKVLRSSVREIAAIYLAAGLDPSRAVIFRQSSVGEHAELSWLLNCIAKV
                     GELSRMTQFKDKTQRGGTEAASVGLYAYPVLQAADILLYNAHLVPVGEDQRQHLELAR
                     TLARRFNHLYGETFVVPEVMIPKTGARIMALDEPTAKMSKSRPTPGGYISLLDEPDEI
                     RRKIRRAKTDSGTEVTASPDKPAISNLLGIYAGLSNSTVQEVEEQYRGKGYGDFKKDL
                     AEVVVEGLSPIRKRTLELLDNPKELDALLESGAEKARAVARPILRDAASRMGLD"
     gene            complement(7879..8718)
                     /locus_tag="VN178_16240"
     CDS             complement(7879..8718)
                     /locus_tag="VN178_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HWS82570.1"
                     /translation="MPEKISVSGLARRPDMTGTLRRLLKLILVVVLLVGASQGTTLAA
                     PTSWDYVALGDSLATGYGAFVKGYVPRYEAYVETDTGVAVTRTNLAQNKWTSSQLLSA
                     LRSDPTFRRAVREAEIVTWNVGGNDLRAARTSYKNGTCGGVDNQDCLRASVATLKTNW
                     TAITAEVLELRSTSDTIVRTMDIYNPYVRTDIVSDTWQNDGGMNDFQVFKKYLDRINR
                     HIATTSYTKGVGYAPVYLAFNGTFGDEDPRSKGYLRLDGLHPNNTGHRIIAGELRFMG
                     YAP"
     gene            9040..9225
                     /locus_tag="VN178_16245"
     CDS             9040..9225
                     /locus_tag="VN178_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82571.1"
                     /translation="MDHQGERTHRKQGFVSVARDIIDSNAYSTPATADENGLLAVRRH
                     QNKVDQDITVVIVECLG"
     gene            9222..10364
                     /locus_tag="VN178_16250"
     CDS             9222..10364
                     /locus_tag="VN178_16250"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWS82572.1"
                     /translation="MMGNDVPGSVLGRRALNRATLARQMLLHRAELPVSEAVAALVGL
                     QAQTTHSWYVGLWTRLEGLEAEAVGKHLEERRLVRLALMRSTIHLVTAEDCLALRPVV
                     QPGIERSVRGQFGRRLEGLDREEVAAAARALVEEQPRTFSELARLLEERFPGRDAFAL
                     GQAVRAWVPLVQVPPRGVWGKSGRAAHTSVEAWLGGVPSPGSSLEDLIVRYLAAFGPA
                     TVKDAQIWSGLTRLREVIERLRPRLITFRDEQGAELFDLPDAPRPDPDTPAPPRFLYD
                     YDNLLLSHSDRSRVITDGYREQNFASDGPMPSIVLLDGFTNATWKITRHRGTATLTIR
                     PFIRLLDEDATALAEEGVRLLAFLTPDADVRDVRFMTDQTEGRPRR"
     gene            10602..12242
                     /locus_tag="VN178_16255"
     CDS             10602..12242
                     /locus_tag="VN178_16255"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWS82573.1"
                     /translation="MPDLIIGPLLRYVGPTDATVWVETDAACEVEVLGCPSPTFRVGD
                     HHYALVHVTGLEPGEACEYEVRLDDRTVWPESGSPFPPSVIRPMKNGEDLRLVFGSCR
                     ISAPHEPPYTLGHDEDGRGLGVDALYATAMRLKDERPEELPHALVLLGDQIYAHKPPF
                     DTLQFIGERRGAEGPPGEVVADFEEYARIYHDAWADPAIRWLLSTVPSAMIFDDHEVG
                     DDWNISAAWVEEMRQRPYWNDQIIGGYASYWVYQHLGNLSPEVLDRDALYKRVRDADD
                     AWPVLRDFAYRAHRGPDGTRWSYHRDFGNTRLLMMDARGGRVLEEDRRSMVDEEEWAW
                     IVEMATGDFDHLLLGTSLPLLLGPTMHHLQSWDEKLCSGTWGARAARWAEGFRRSEDL
                     DHWASFHDSFTAMVGLIQRIASGDTGTPPSTILILSGDVHHGYLAEATLAGSAQSRIY
                     QAVCSPLRNALPSKKSYLQSHAWSKPATLATRLLARLAGVRRVPLAWRLTHNATFFEN
                     QVATLVFEGRHATITYEKAVLDASNEPALKGLYQFRLA"
     gene            complement(12279..12992)
                     /locus_tag="VN178_16260"
     CDS             complement(12279..12992)
                     /locus_tag="VN178_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWS82574.1"
                     /translation="MDSVEVLTSGSKSGVGVLDKSVAILAFLSGGPATLPEVVEGTGL
                     PRSTAHRLVSALETHHLVSRRDGRYVLGLRLLGWGNRAAGVGLVERARPVLEELRDES
                     GESTQLYVREGGQRVCVAAVERVTGLRDTVPVGAVMPLERGSAGKVLLAWAPGGVDGR
                     PDAAQLRGIRVRGWAESVAEREAGVASVSAPVFGEGGRLRAAVSVSGPISRLGESPGR
                     RLSQQVVGAARKIEGDLAP"
     gene            13037..13819
                     /locus_tag="VN178_16265"
     CDS             13037..13819
                     /locus_tag="VN178_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWS82575.1"
                     /translation="MILARDWDAGEYEKLSAPQTRWGVEVLGRMDVRGDEAAIDAGCG
                     TGRVTELLLAKLPEGSVLAIDGSRAMVEAARRRFAGEPRVRVERQDLLSLEVEERVDL
                     IFSTATFHWIWDHDRLFENLARALKPGGRLVAQCGGEGNISRATKATIETMEEERFLD
                     YFVGWYDDKYYADARTTARRLEAAGFEEVETWLHDEVAAFDTVDELARFLGTVVLGGH
                     LEKLPEEVRSPFAARVAEKVAAVDGRPALDYVRLNIMARRSA"
     gene            13816..14526
                     /locus_tag="VN178_16270"
     CDS             13816..14526
                     /locus_tag="VN178_16270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWS82576.1"
                     /translation="MTVGTPFGQAAGDRPELVPGFDRFYGAVLESIPFREEQEIRVLD
                     LGAGTGLLSAVVAGKFPRARVTLVDLSVEMLRVARRRFSGERGRFEFRTMDYARKPLP
                     RTEGGYDLVVSALSVHHLTHGDKRELFEKVHRSLAGGGYFVNADQISGETPEEEALYR
                     EWWLRRVREVGVSEEDLAAAVSRMRADRNATLGAQLFWLGEAGFEGIGCRYKDHRFAV
                     YNGRKGNENSENGKDADG"
     gene            14519..15922
                     /gene="leuC"
                     /locus_tag="VN178_16275"
     CDS             14519..15922
                     /gene="leuC"
                     /locus_tag="VN178_16275"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWS82577.1"
                     /translation="MDRPKTLVEKVWEKHVVRSAEGEPDLIYVDLHLVHEVTSPQAFE
                     GLRLAGRGVRRPDLTLATMDHNVPTSAFGLPIEDKVSARQMEALRSNCEEFGIKLEEW
                     GSIGQGIVHIIGPETGLTQPGLVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     TQSRPKTMAVTVEGELPADVTAKDLMLGILNRIGTGGGVGHVIEYRGEAVRQLSMEGR
                     MTVCNMSIEGGARAGLVAPDETTFEYLRGREYAPKGEHWEAAVEEWKKLPTDDGAEFD
                     KEVVIQAEDLVPYVSWGTTPAQTVPLDGEVPEPQNEGHERALKYMALDPGTLIRDIEV
                     DTVFLGSCTNARIEDLRAAAGVLEGHKVKEGIRAMVVPGSMRVKKQAEEEGLADIFTE
                     AGFDWRNAGCSMCLGMNPDILQPGERCASTSNRNFEGRQGKGGRTHLVSPVVAAATAV
                     MGRFASPSELGVPLEVG"
     gene            15922..16509
                     /gene="leuD"
                     /locus_tag="VN178_16280"
     CDS             15922..16509
                     /gene="leuD"
                     /locus_tag="VN178_16280"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWS82578.1"
                     /translation="MKPVERISGTALPLGYSDVDTDQIVPSDALKRIERTGFGQFLFS
                     GWREDEDFIMHREEYKDAVILIAGENFGSGSSREHAVWAIQDYGFGAVIAPSFADIFR
                     NNSTKNGLLAIDLPEETVQNLLRAVREDPETVITIELDELTVTAPNVHESFEMDDFTR
                     RRIMEGLDDISLTLTHEDELDVFERSRPDYLPTVQ"
     gene            16621..17544
                     /locus_tag="VN178_16285"
     CDS             16621..17544
                     /locus_tag="VN178_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWS82579.1"
                     /translation="MLEVAIMVEGQEGVGWDRWRRLALAVEDLGYAGLYRSDHFPTKQ
                     SDIYRDGLELWSSLTWLAEHTSRIEFGPLVSPVSFRHPVITAWQASSVDNLAGGRLRL
                     GLGAGWNEWEHGTFGFDVLDTDRRFARLEEGLEVVTRLLRSDEPVSFHGEFYQLNDAL
                     LMPRSPRPGGTPVVVGGNGVRRTLPLAARYADEWNAVFLRAEAFVDLNARLDELVREA
                     GRAPEQVRRTLMTRVIFGRTEAEVGRKLEGEFRDHLPAAVLAGTTGEIVERLGHLSEA
                     GVQRVMLQWLEVDDIDALEAMAHSVLPQLRG"
BASE COUNT         3010 a         5612 c         5695 g         3278 t
ORIGIN      
        1 cccgtacccg ctcgactacg aagtcggcgg gcgtcggcac ctcttccggc agagtcgata
       61 ggtccacggc catcccaatg tcgtcgccgc cgtactcgaa gccgtgcgcg atgaggcgtt
      121 cgccgaggtc ggatgggcgc atcgagggtc cgacgtgcca ggagccgggg accccgtgtg
      181 cccgcattcg ttccagtgag gcttcgatct ccccatccgc ctcttcccgg gtcaggtcgg
      241 cctgtacgac gcagttgtgg tagtcgatgg gggagttccc gatggtccac cgaacccggc
      301 cgtcgtcgcg ttcctcggcg cctgccgccc gtccgagcgc cagcaggaac tcggcgccgt
      361 tctcctcgat ggcctggatg acggacggag agtccggatc atgatccttc aaccagcctc
      421 cttgggacga ggagttcgca cacagggacg gaccgcgtgg cctccttatt cacgaaccgg
      481 gtgcgaatcg aggagccacg ccagagtcac agggtccctc gtcgcaccgg ttctctccag
      541 cgtcaccgtc gcaggcggtg ggcgagcggc gagaaatgct gcgctttgtg cacctcgata
      601 tccgccgcat cctgcctctt catcggcttc tcccgttcgg cgggccgggg cctgtctatg
      661 tagcgtcccg gtatcacgct cgataccttg gccttattag aagcggtcct gccgttatcg
      721 cttgccttta cgggtacccc ggcgtcgcgg gcgccctgga gggcatgctc gtaggcctcg
      781 cgggctttct cgaaggtgcc ggcgtagttg tgtcgggcta gcgccgcctt cgcctcgccg
      841 taggccacgt cggccgccgc gagctcggcc gccgcctggt cggcgtcggg atcgttctgg
      901 atcttgcgcg ccagggcgtt ggcgttgttt atgtaggccg ccgcctggaa gcggtcggtg
      961 ttgtcccggt cgaactggga gaagtcccag ttgaggtcca catagctcat tatcgagttg
     1021 acctcgttgc cgaccccggt gaagtagtac tttccgtccc agccgaaatc cgtttggttc
     1081 tcgtaatcga agccgtcgaa cgggtggctg aggttgaggt ggtggccgta ctcgtggatc
     1141 tcggtggtcg tgagcccata gttcttcgct atgtccggcg agatgaagct gaaggcgaag
     1201 ctctgggtac cgtcgcggtg attgtcgtcg gcgaggccga gcacgggggg cgaggtcagg
     1261 cccttcggac cgtctttgac ggcccagttt agtagtgccg cctcgtattc gcgggccgtc
     1321 cgttcgtcgt ccagcagtct gctgaggttg ttagcgccgt agaggaacag gttggcggtc
     1381 cctgtatagg cggggaactt cttgaggtag catggcacat gcgcccgcca gagcaagtag
     1441 cagcgcttcg ccttgccctt gtaggctaca tcttgctggt ctacggagta gggcaggcgg
     1501 tgtagctctc tgatctcgtc tagtagcaag ctcggggtct ggtagcgtct cgagtagttc
     1561 ttacctggga tgccctcgac ggtgttgacg tccaggttga tcttattcgg caagagccgc
     1621 ggcgtgaact gcggcgggta gatcggcgag ctggtgaaga acaggtcgat ggcgacgtaa
     1681 cgcgtaacct tggataggtc gccggtgagc ctcgaggccg ggcggtagcc atccttggcg
     1741 tagtactccc aaacgggcgg cagccggtag tcttccttgc catcccccgt gaggtcgggg
     1801 tcgtcgacat tccagttgtc ggtgaacgag tccggtcccg ccgagaggtc gtggaaccag
     1861 atgcgccgcg tggagcccag gccgctctcc tcgtcgtcgg cggtggtgcc gccccaggca
     1921 atgagcttgc ggctgtcgtc cctgccgaag ttgaagctgg cgtcgggttc gggctcgttc
     1981 gtcttggtgt aaacgtggtg cttgaagccc ttgcgcggct tctccccgtc gccccaccag
     2041 ttgatgaaga agacggtgtt gcgctttgta tcgataccgg ccggcggatt cgctgcgagc
     2101 cactcctcga cggatagggc gcttatgcgg tagttcttct tgatatcgcg gaccccctgc
     2161 ttttggcagg gcttgggctc tccgttcggt ggtttgcaga acttgcgcgg ttcgccgtta
     2221 tacagtttct ggaaatccgt tagcggcgcc ggcttcgccg catccgacag gtagctgaag
     2281 aacctcctct cataggctcc gttggcgaat ctgacgttat agtcgtaggt gtagttcaag
     2341 ccgagcagcg acttcttgac gctcttgttg tagaagtacc tggagcggac tatcggcttg
     2401 tactggtctg gaagcgtcga gcggaatctc ccggcgctca cgtcatcctg gtcgtaacct
     2461 acgaacacga cgttcaccga agccttctcg tctagatccg cacgcttgcc aggttccagg
     2521 ttctggtaat cgccggcctg accgaagccc gcggccggta ccccgccgag agccaacacg
     2581 ccaatcccga acgcagccag gtaacgtctc agtctcttgc ccaacactcc tcctccagtc
     2641 gtcgcccgcg taccagtcat tgggtctccg aaccgcatag cgctgacttg actctttccc
     2701 ctggcagtag agccgtttca cgaggtccga ggggccgact gccagacacc tactgaagca
     2761 ttgtagctag ccttgctgcg acagtaaacg cccttcttgg cggcacgact ccctagagcg
     2821 cgtagtcggc gtctgaggcc tcttcgaggt agcgggcgag gagtgttatg gcggcctcca
     2881 cgtccttggc gctcaccatc tcgttcgggg tgtggacgta acggcagggg atggagagcg
     2941 tgacggccgg gatgccgccg tgcaggcgct gtacgccgcc cgcatcggtg ccgccagcgg
     3001 ggagaatctc catctggtag gggatgtcct cgcgctcggc gatggcacgg agatgctcca
     3061 ccaacttcgg gtgggagatg gagtagccgt ccatgatctt gatggcaaca cctttaccca
     3121 gctcggttat ggtgttctcg ttcccgccgc cggggacgtc catcgcgagc gtcacgtcga
     3181 gggcgactac gaccgtaggc tcgagggcag atccgctggc ctccgcgccg cgcaggccga
     3241 cctcttcctg ggaggtggcg gcggcgaaga tagtcgcctg atggtctccc acagccctca
     3301 gtgcttcgta catcacgaag acgccgacgc ggtcgtccat cgccttgcct acgtaggtct
     3361 cgccgacctt ctccagggtg cggtccatga ccgcatagtc cccgatgcgc accttctcct
     3421 tgacggtctc ggcgtccatg ccgaggtcga cgtagaactc gtctgccttc ggcggtttct
     3481 gctcctcgcc gcgcgtcacc aggtgcggtg gcttgcgcgc cggctggagc gcgccgcgga
     3541 gcgtctcgcc atcggcggtc gttacgagga ccctctggga gaccatgttg gcgggatcgt
     3601 gacctcccag ggtctgcagg cggacgaagc ccttgtcgtc gatgtgtttg acgaggaagc
     3661 cgatctcatc cgtgtgcgcg gcgatcatca cgcgcccgtt gccgctcccc tttttcgtcc
     3721 cgatcacact gcccagggcg tcggtgcgga cctcgtccgt cagagagcca agctcctcgc
     3781 gtgcgatctc acgctgccgc tcctcccgtc cgggggcgcc cggcgtctcg atcaaccgtt
     3841 tgagtagtgc ttcgttcagc gccatgccat tcctttccag aggatacgca gggagcctaa
     3901 caacagcgcg aatgcgcgtc aaagtccctg gaatggtacg gtacggatcg acgtaacttc
     3961 gcggcccgta ggaccttctt agtctgcggt ctcctcttcc aggcgctggg cttcgagcag
     4021 tttgcccgag aggtagaggc tgaggccgaa gtagtagacg aggtagagga tggagaaggc
     4081 caggatcggg ggatccgggt cgggcatctg ccagagtatc gtggcgtagc tcgcgagcca
     4141 cagggccgtg agggtcaggg agagcttgcg gaaggcgacc gtgcgcgtcg ggtagacgta
     4201 gcggatcggg acgaagacca ggaacgagca ggccacgagc acggcggcga gcaccagcgg
     4261 gcccggatgg aagacgatgg cgtagaaggc gacgacgttg aaatagctgg ggaagccgag
     4321 gaagtagtgg tcctcggtct tggcgtcgac gcggcagaac tggtagctgg atgccagaag
     4381 cggcagcgcg gcgagaacta tgccagcgtt gccggcgggc aggtagcctt cgctccagag
     4441 caggaccacg ggggcgaaga cgtaggtcat gtagtcaacg atgttgtcca aaagcgctcc
     4501 gtcgaagaag gggagcgcct cgctgaccct gaaacggcgg gcgaggagac cgtcggtaga
     4561 gtcgatgagg agcgaggcca gcatcagcca cagcgccttt acggcctcgc cctggaaggc
     4621 ggccacgagc ataaggagcg cgagcacggc gccgctcgcc gtgtacaggt gcacggccac
     4681 ggccttcgac ctgaaccagg agggcgtctc cgggttaccc gcgtcggtag cttcctctac
     4741 ttgatgttcc ctggtctccg ccacgctcag tgcctaatga taccagccat aacagtatgt
     4801 cttctacatc ttggggacgg cccgcagcgg acgtatgcgg tttgtggcgg cagcgacttc
     4861 ctgggcgcgc ggcaggctca tctcacccgg gtggcccccg tggccaccca gggagtcgag
     4921 ccctttctca gagacggagg ccagcgcatc cctgacgagc tcgcccacct cgctgacctc
     4981 accggtggcc gcccactccc cgatgatgcg ggccaccgcc tcggtctgtc cggcctcggc
     5041 gagctgctcc aggtaggaga ggtcgacccg ctcacggccc agttcgatgg tgtggagtcc
     5101 cctgccgcgg acggaccgtc gtttgccccg tctcaggtct atcgaggaag cgtcgatggc
     5161 gcgctcttgc ggcggaagca cctccctgtc cgcaagcggg gcacggggcg ggaacctctt
     5221 ggttacctca cgggagcggg gtgtcgcgtc ggaaggtgcg tagtcctcca agaggatcac
     5281 acggtctgca acgtcgaggt agtctcccac tccgcccacc accacgaccg tggagacgcc
     5341 gagagagcgg tgcaaggggc gcaccaggtc tataaaaggc gagatgggct cgcgcctcac
     5401 cagctcgcgc atcctctcgt ccctgatcat gaagttcgtc gcgctcgtat cctcgtccac
     5461 gagcaacagc gacgtcccta cctcgatcgc ctcggcgatg ttcgcggcct gggaggtcga
     5521 gcccgaggcg ttcggggtgg agaagtcctt cgtgctccgg ccgccaggga ggtccccgat
     5581 catggccgag atgtccaccc cggagacgct cctgccgtcc tcggccctga ccttgaccgc
     5641 atccctccgc gccacgacca gctccctgcc gtcacccggc acatggtcgt aaacgcccca
     5701 cgagagcgcc gagagcagcg tgctcttgcc gtggaacccg cccccggcca cgagggtgac
     5761 gccctcggga actcccatcc ccgaaacgaa gcccccgttg ggaagttcca cctccaccct
     5821 gtactcctcc gggctaccga acggtacggc accatccctc agtggtctgt cgctcgcccc
     5881 gctctccctc ggcaacaccg ccccgtcagc cacgaacgcg acgagaccca gcccgggaag
     5941 taacccgcgc aggtggtccg cgtcctcgac agcctcgacg tggagcatcg ccctctctac
     6001 gtctacgccg ccctcggcgg ctggaacgag tgccctgcgt acgacctcgg gcagttcttc
     6061 gagcagcact gctttggccg cgcgggcgtc gacgcttcgg ccccgggcgg gtaggcccac
     6121 ggccatgcgg gcttcgatgt agttaggttc cacgaccatc gaggtgcggg ggaggacgac
     6181 ctgggtggtc cgctgtatct cgacccgtcc gctcccgccc gagccccggt taccgcgcac
     6241 ggtgcgccgg atctccctct ctacgctcct ggtgaggaaa tcctcgaaag cgactctgcg
     6301 caccagattc tcgaagagag caggttcgaa agagttcgct gtggttcgga ttcggatcag
     6361 ggaaggcgga gcgaagggat cccgctgcac tctgtctaca aacaggacga aagagctgaa
     6421 gtcgtgagag ccggagaggt ctttgtaggc tccgtaaccc ttgcggtcta tgcggtcgag
     6481 cgtcgaacgc aggcgttgca tcgtatgagg ttatacagcg tcctggtgcc cgcgcctggt
     6541 ccgttcttct tcgatctcct tcttggcctg ttcccacggg atcacttcgt cttccccacg
     6601 ttcgagttct gcctttgcct catcgtaagc ctgagtcgcg agtacgtctt ccagttcctc
     6661 caactcttcg atcaggcgct cgtattcctc gattggcagg atgacgctta cgcgctcacc
     6721 gttctcgtct atcacgtatt gtgcacccat cgtcacacct ccctcccaag ttttaggctt
     6781 tcttggtatc ttcctttagt ctagccccat ccttgaagcg gcatcgcgga ggatgggacg
     6841 ggcgacggcc cgggccttct cggcgccgga ttccagaagg gcgtccagtt ctttcgggtt
     6901 gtccagtagc tccagcgttc gcttcctgat gggggagagg ccttccacga ccacctctgc
     6961 gaggtccttc ttgaagtccc cgtagccctt gcccctgtac tgttcttcga cctcttgaac
     7021 agtactattc gacagcccgg cgtagatacc gagaaggttg gagatggccg gcttatcggg
     7081 ggaggcggtt acctcggtgc cggagtcggt cttcgcgcga cggatcttgc gccgtatctc
     7141 gtcgggctcg tcgaggagcg agatgtagcc ccccggggtc gggcggctct tgctcatctt
     7201 ggcggtcggc tcgtcgaggg ccatgatcct cgctcctgtc ttggggatca tgacctcagg
     7261 gacgacgaag gtctcgccgt aaaggtggtt gaaccgccgc gcgagcgtcc gggcgagctc
     7321 gaggtgctgg cgctggtcct cgccgacggg tacgaggtgg gcattgtaga ggaggatgtc
     7381 ggcggcctgt agaacaggat aagcgtagag tccgacgctc gcggcctcgg tcccgcccct
     7441 ctgcgtcttg tccttgaact gtgtcatgcg agagagctcg cccaccttgg cgatgcagtt
     7501 caaaagccag ctgagctccg cgtgctcgcc gacgctgctc tggcggaaga tcacagccct
     7561 cgaagggtcg aggccggcgg caaggtagat cgcagcgatc tctcgcacgc tgctcctcag
     7621 caccttcggg tctcgcggcg tcgtgagcgc gtgcaggtcc acgatgcagt agaacgtctc
     7681 gtagtcatcc tggagggcga cgaagttctt caacgccccc agataggtgc ctagatggga
     7741 cgtgccgctc ggctgtatcc cgctgaatac tcgctccttg ctttgcatgg gcgttattct
     7801 aacggactcg gaggaggttg gaccggcttt ccctcggtac cggcccgacc tctatctacc
     7861 ggggatccag acctatccct atggagcgta gcccatgaac cgtagctcgc cggctatgat
     7921 cctgtgtccg gtgttgttgg gatgtagtcc gtctaacctc agataaccct tggatctcgg
     7981 atcttcatcg ccgaacgtcc cgttgaaggc cagatacacc ggggcgtacc ctacgccctt
     8041 cgtgtaagag gtggtggcga tgtgacggtt gatcctgtcg agatacttct tgaagacctg
     8101 gaagtcgttc atcccgccgt cgttctgcca ggtgtcggag actatgtcgg tcctgacgta
     8161 ggggttgtag atatccattg ttcgcacgat cgtgtcgctg gtcgaacgca actcgagaac
     8221 ctccgccgtg atggcggtcc agttggtctt gagcgtcgcc acgctagccc gcaggcagtc
     8281 ctggttgtcg accccaccac aggttccgtt cttgtacgag gtacgggccg ccctgagatc
     8341 gttcccgcct acattccagg tgacgatctc cgcctcgcgg acggcgcgcc ggaacgtcgg
     8401 gtcgctgcgg agggcagaga gcagttggga actggtccac ttgttctggg cgaggttcgt
     8461 gcgtgtgacc gccaccccgg tgtcggtctc gacgtaggct tcgtagcggg gcacgtagcc
     8521 cttgacgaag gcaccgtagc ccgtggccag ggagtcgccg agggccacgt agtcccacga
     8581 ggtaggtgcg gcgagagtgg ttccctgcga cgcaccgacg aggaggacca cgacaaggat
     8641 gagcttcagc aagcgccgca aggtgcctgt catgtctggt cgtcgtgcga ggcccgagac
     8701 actgatcttc tccggcataa ccccctccta tgtcagccta agttcgctgt gttgcatcgg
     8761 aactctacac tatcgaggag aataggtgta acccctaatg catcatcttc ttggaggtct
     8821 cggcgacatc tgtttcccga ttcgactacc aaaacgctca gaagtctacg atatcaaggg
     8881 cgacacgctc gcgatctggt tcgaaggcag gagctcactc gcgtaccatg ggagcacctt
     8941 cggcggggac ggcgatttcg gccaggatcg ggtagagttg gcatcccgac ggagtggcgc
     9001 agggtacggg cggggagcgt gggttaggta gggaagagag tggaccacca gggggagagg
     9061 acgcatcgca aacagggctt cgtgtccgtc gcgagggata tcatagactc caacgcttac
     9121 tcgacgcccg ccacggccga cgagaacgga ctcttggcgg tacggcgtca ccagaataag
     9181 gtcgaccagg acatcactgt ggtgatcgtg gagtgcctcg gatgatgggt aacgacgtcc
     9241 ccggttctgt gctcggccgg cgcgcactca atcgcgccac gctggcacgc cagatgctcc
     9301 tgcaccgtgc ggagctgccc gtttccgagg cggtcgcagc gctcgtcggc ttgcaggccc
     9361 agacgacgca ctcgtggtac gtcggcctgt ggacgcggtt ggaaggtctc gaagcggagg
     9421 cggttggcaa gcacctagag gagcgtcggt tggtgcgcct ggccctcatg cgctccacca
     9481 tccacctggt gaccgcggag gactgcctgg ccctacggcc cgtggttcag cccgggatcg
     9541 agcgcagcgt caggggccag ttcggccggc gcctggaggg gctggaccgg gaggaggtgg
     9601 cggccgcggc cagggcgctg gtcgaggagc aaccacgcac ctttagcgag ctggcgaggc
     9661 tgctcgagga gcgcttcccc ggtcgcgacg ccttcgcact gggacaggcc gtccgcgcct
     9721 gggtgccgct ggtgcaggtg ccgcctcgcg gcgtctgggg caagagcgga cgtgccgcac
     9781 ataccagcgt cgaggcgtgg ctcggaggag ttccctctcc cggctcctcg ctcgaggatt
     9841 tgatcgtgcg ttacctggct gcattcggcc ctgctaccgt caaggacgcg cagatctggt
     9901 cgggactaac ccgtctgcgg gaggtgatcg aacggctccg gccgcgtctg atcaccttcc
     9961 gcgacgagca aggcgccgaa ctcttcgatc tgcccgatgc cccgaggccc gaccccgata
    10021 ctcctgcccc gccgcgcttc ctctacgatt acgacaacct gctgctctca cactctgacc
    10081 gctcccgagt catcacggac gggtaccgcg agcagaattt cgcctccgac ggcccgatgc
    10141 ccagcatcgt gctcctcgac ggcttcacta acgcaacctg gaagataacg cggcaccgcg
    10201 gcacggccac gctcaccatc cggcctttca tacggttgct cgacgaggat gctaccgctc
    10261 tcgccgagga gggcgtccgg ttgctagcgt tcctgacacc cgacgccgac gttcgggacg
    10321 tccggttcat gaccgaccaa accgaaggga gaccacgccg atgacgggcg atgcccctcg
    10381 cggagccggg atccagccgt tccggaggcg ccgggacggg tccgcgctac tcgaaggtac
    10441 aggaagctac ggcgtcgggt cctcacgctt ctcgaggtcc cggaggttgt gcgtggtcga
    10501 gaagtctcgg cacgccgcac cctcgcgcga ctttcgggcg tcgagtacga tggcgccgtc
    10561 ttgtcatgca ctagcgccac cgcaccctgg cccggattac cgtgcctgac ctcatcatcg
    10621 gcccgctctt gcgatacgtc ggtcctacgg acgcgaccgt ctgggtcgag accgacgctg
    10681 cctgcgaggt ggaggtgctt ggttgcccct ctccaacttt ccgcgtcggg gaccatcact
    10741 acgctctcgt ccacgtcacg ggcctggaac ccggagaagc ttgcgagtac gaggtgcgcc
    10801 tcgatgacag gacggtctgg ccggagtccg gctccccctt cccgcccagc gtgatccgtc
    10861 ccatgaagaa tggggaggac ctgaggctgg tcttcggctc gtgccgcatc tcggcgccgc
    10921 acgagccccc ctataccctg ggccacgatg aggacggacg cggcctcggg gtcgacgccc
    10981 tctacgctac tgccatgagg ctaaaggacg agcggcccga agaactgcct catgcgctcg
    11041 tactcctggg ggatcagatc tacgcccaca aacccccctt cgacaccctg cagttcatag
    11101 gggagcgccg cggcgcggaa ggtccaccag gcgaggtcgt cgccgacttc gaggagtacg
    11161 ccaggatcta ccacgacgcc tgggccgacc cggccatccg ctggctcctc tctaccgtgc
    11221 cctcagccat gatcttcgac gaccacgagg tcggagacga ctggaacatc tccgcagcct
    11281 gggtcgaaga gatgcgccaa cggccctact ggaacgacca gatcatcggc ggctacgcct
    11341 cgtactgggt ctaccagcat ctcggcaacc tctcccccga agtactggat cgagacgctc
    11401 tctacaagcg tgtcagggat gctgacgacg cctggccggt cctgcgcgat tttgcctacc
    11461 gcgcccatcg cggcccggat ggcacccgct ggagctacca ccgcgacttc ggcaacacgc
    11521 gcctcctgat gatggacgcc cgaggcggcc gcgtgctgga agaggaccgt cgctctatgg
    11581 tcgatgagga agagtgggcc tggatcgtcg agatggccac gggcgatttc gaccacctcc
    11641 tccttggcac ctcgctccct ctgctactcg gtcccacgat gcaccacctc cagagctggg
    11701 acgagaagct atgctcgggc acctggggag cccgggccgc ccgctgggca gagggtttcc
    11761 gccgctccga agacctcgac cactgggcgt ccttccacga ctcattcacc gctatggtag
    11821 gcctgataca acgcatcgcc agtggagaca cgggaacccc accttcgacc atcctgattc
    11881 tctccggcga cgtccaccac ggctacctcg ccgaagctac tctcgccggt agcgcgcaga
    11941 gccgcatcta ccaggccgtc tgctcccccc tgcgcaacgc cctcccgagc aagaagtctt
    12001 acctccagag ccacgcctgg tcgaaacccg ccaccctcgc cacgcgcctc ctggctcgcc
    12061 tcgcgggcgt ccgcagagta cccctcgcct ggcgcctgac ccacaacgcc accttcttcg
    12121 aaaaccaggt cgccacgctg gtgttcgaag gccggcacgc caccattact tatgagaagg
    12181 ccgttctcga cgcctcgaat gaacctgccc taaagggact ctaccaattc cgtctagcat
    12241 agagggttct ggccaaatct ccagccgacc gtacagagct aaggggccag atccccttct
    12301 atcttccttg ccgctcctac gacctgctgg gagagccgcc ggcccggact ctcccccaga
    12361 cgtgagatcg gaccactgac gctcaccgcc gccctcagcc ttccgccctc cccgaataca
    12421 ggcgcgctga cgctggccac cccggcctcc cgctcggcca cgctctcggc ccagcctctg
    12481 acccggatcc ctcgcaactg ggccgcatct ggccttccat cgacccctcc aggcgcccac
    12541 gccagcaaga ccttccccgc cgaaccccgc tccagcggca tgacagcccc aactggtacg
    12601 gtgtccctga gaccggtcac ccgctccacc gcggccacgc acaccctctg cccaccctcc
    12661 cgcacataga gctgcgtgct ctccccactc tcatcccgca actcctccag caccggccgc
    12721 gcccgctcca cgagacctac cccagcagcc ctgttccccc aaccaagcag cctcaagccg
    12781 agtacgtatc gcccatcccg ccgtgatacg aggtggtgcg tctccagcgc cgatacgagc
    12841 ctgtgcgccg tggaacgcgg caacccggtc ccctcgacca cctctggcag cgtcgcagga
    12901 ccccccgaga ggaaagcaag gatcgctacg gacttgtcca gtaccccgac cccgctcttg
    12961 cttccggatg taagaacttc tacactgtcc atatgctgat cattatgtct cacattttga
    13021 gacaacacaa gaaggtgtga tcctggctcg cgattgggac gcaggcgagt atgagaagct
    13081 ttctgcgccg cagacccgtt ggggggtgga ggtcctgggg cgaatggacg tgcgtgggga
    13141 cgaggctgcg atcgacgctg gttgtggcac cggtcgggtg acggagctat tgctggcgaa
    13201 gttgcccgag gggtcggtgc tggccataga cggttctcgg gcgatggtcg aggcggcgag
    13261 gaggcggttc gcaggagagc cgagggttcg ggtggagcgc caggatctct tatcgctcga
    13321 ggtggaagag cgggtggatc tgatcttctc cacggcaacg ttccactgga tatgggacca
    13381 tgaccgtctc ttcgagaacc tggcgcgggc cctaaagccg ggtgggagac tcgtcgccca
    13441 gtgtgggggc gagggtaaca tctcccgcgc cacgaaggcg acgatagaga ctatggaaga
    13501 ggagcgcttt ttggactact tcgtgggctg gtacgacgac aagtactacg ccgatgcgag
    13561 gaccacggcg aggcgtctgg aggccgcggg cttcgaagag gtcgagacct ggttgcacga
    13621 cgaggtcgcg gcgttcgata cggtggacga gctcgcccgc tttctcggga cggtcgtgct
    13681 ggggggacac ctggagaagt tacctgagga ggtgcgatcg cctttcgcag caagggtggc
    13741 ggagaaggtg gctgcggtgg acggcaggcc cgccctggac tacgtccgtt tgaacatcat
    13801 ggcgaggagg tctgcgtgac ggtcgggaca cccttcggtc aggctgcggg agacaggccg
    13861 gagctcgtgc ccggtttcga ccgcttctac ggggccgtac tggagagtat ccctttccga
    13921 gaagaacagg agatccgggt actcgacctt ggggccggga cgggactcct tagcgcggtg
    13981 gtggcgggga agtttccgcg ggcgcgggtg acgctcgtcg acctctccgt agagatgctc
    14041 agggtggcgc gccggcgttt ttcgggggaa aggggccgtt tcgagtttcg gaccatggat
    14101 tacgcgcgca agcccttacc ccgaaccgaa gggggttacg atctcgtggt gtcggcgctc
    14161 tcggtgcacc atctcacgca tggcgacaag agggagctgt tcgagaaggt ccaccgttcg
    14221 ctcgccggag gcggctattt cgtcaacgcg gatcagatct caggcgagac gcccgaggag
    14281 gaggcgctct acagggaatg gtggctgcgg cgggtgcgcg aggtaggggt ctcggaagaa
    14341 gacctggccg ctgcggtctc gcgcatgagg gccgacagaa acgcgacgtt aggagcgcag
    14401 ctcttctggc tcggggaggc cggtttcgag gggatcggct gtagatacaa agatcacagg
    14461 ttcgcggtct acaatggtag aaagggaaac gagaactcgg agaacgggaa ggacgctgat
    14521 ggataggccg aagacgctgg tagagaaggt gtgggagaag cacgtggtgc ggagcgccga
    14581 gggtgagccc gacctcatct acgtcgacct gcacctggtt cacgaggtga cgagcccgca
    14641 ggctttcgag ggcctcaggc tcgccgggag gggggtgcgc cgtcccgacc tgaccctggc
    14701 gacgatggac cacaacgtcc cgacctcggc gttcggattg cccatcgaag acaaggtctc
    14761 ggccaggcag atggaggcct tgaggagcaa ctgcgaggag ttcggcatca agctcgagga
    14821 gtggggctcc atcgggcagg gcatcgtgca cattatcggg ccggagacgg gcctcaccca
    14881 gcccgggctc gtgatcgtct gcggcgactc ccacacggcc acccacggtg cgttcggcgc
    14941 gctcgcattc ggcataggga cgagcgaggt cgagcacgtc ctcgccaccc agacgctcac
    15001 ccagagcagg ccgaagacca tggccgtcac cgtcgagggc gagctccccg cggacgtcac
    15061 ggccaaggac ctcatgctcg gcatcctgaa ccgcatcggg acgggtggcg gggtcgggca
    15121 cgtcattgag tacaggggtg aagccgtcag gcagctctcg atggagggac gcatgaccgt
    15181 gtgcaacatg tccatcgagg gaggggccag ggcaggcctc gtggcgcccg acgagactac
    15241 cttcgagtac ctcaggggca gagagtacgc cccgaagggc gaacactggg aagcggcggt
    15301 cgaggaatgg aagaagcttc cgaccgacga tggcgccgag ttcgacaagg aggtcgtgat
    15361 ccaggccgag gacctcgttc cctacgtctc ctggggcacg accccggctc agaccgtccc
    15421 gctcgacgga gaggtcccgg agccgcagaa cgaaggccac gagagggcgc tcaagtacat
    15481 ggcgctcgat ccgggaaccc ttatccggga tatagaagtg gataccgtct tcctcgggtc
    15541 gtgcaccaac gcccgcatcg aggacctgcg cgccgccgcc ggcgtcctgg aaggccacaa
    15601 ggtcaaggaa ggcataaggg cgatggtcgt cccagggtct atgcgcgtaa agaagcaggc
    15661 agaagaagag gggctcgccg acatcttcac ggaggccggc ttcgactggc gcaacgcggg
    15721 gtgctcgatg tgcctcggca tgaacccgga catactgcag cccggcgagc ggtgcgcctc
    15781 gaccagcaac cgcaacttcg agggacggca gggcaagggt ggcagaacgc acctggtcag
    15841 ccccgtggtg gccgctgcga cggccgtgat gggccgcttc gcctcgcctt cggagctcgg
    15901 cgtacctttg gaggtgggct aatgaagcct gtcgagcgca tctccgggac ggcgcttccg
    15961 ctcggttatt cggacgtgga cacggatcag atcgtcccta gcgacgccct caagcgcatc
    16021 gaacgcaccg gcttcggcca gttcctgttc tccggctggc gcgaggacga ggacttcata
    16081 atgcacaggg aagagtacaa agacgccgtg atccttatcg cgggcgagaa cttcggctcc
    16141 ggttccagcc gtgaacacgc ggtctgggcc atacaggatt acggtttcgg agcagtgatc
    16201 gccccttcct tcgcggacat tttcaggaac aactcgacca agaacggcct cctcgccatc
    16261 gacctgccgg aagagaccgt ccagaacctt ctgcgggccg tcagggaaga cccggagacc
    16321 gtgatcacca tcgaactcga cgagctcacc gtcaccgccc ccaacgttca cgagagcttc
    16381 gagatggacg acttcacccg ccggcgcatc atggaaggcc tcgacgacat aagcctcacg
    16441 ctaacgcacg aggacgagct tgacgtattc gagagatccc gcccggatta cttgccgacc
    16501 gtgcagtaga gccactcgaa cgaaagctcc tttggggcaa cggtcgctgc actcggtgta
    16561 aggttttccc cggtagcagc gcggcgctac gatgactcga cgtccgaaga ggagggttca
    16621 gtgctggaag tcgcgatcat ggtcgagggg caggagggtg ttggctggga ccgctggcga
    16681 cggctggcgc tggcggtcga ggacctcggc tacgctggtc tctaccgatc cgaccacttc
    16741 cccaccaaac agtcagatat ctacagggat ggcctggagc tgtggtcttc gctcacctgg
    16801 ctggcggagc ataccagccg tatagagttc ggtcctctgg tctcgcccgt ctcgttccgc
    16861 catcccgtca ttacagcctg gcaggcctcc tccgtggaca acctggccgg cggacgccta
    16921 cggctcggcc ttggcgctgg ctggaacgaa tgggagcatg ggaccttcgg cttcgacgtg
    16981 ctcgacaccg accggcgctt cgcccgcttg gaggaggggc tcgaggtcgt gaccaggttg
    17041 ctccgcagcg acgagcccgt atctttccac ggcgagttct accagctgaa tgacgccctg
    17101 ctaatgccgc gctcgccgcg tccaggcggg acgcctgtgg tggtcggggg caacggtgtt
    17161 cgaaggaccc tgcccctggc ggcccgctac gcggacgagt ggaacgccgt cttcctgagg
    17221 gcggaggcct ttgtggatct caacgccagg ctcgacgagt tggtgcgtga ggcggggagg
    17281 gcgcccgaac aggtgcgcag gacactgatg acccgcgtca tcttcggtcg cacggaggcc
    17341 gaggtaggac gcaagctcga aggagagttt cgggatcacc tccccgccgc cgtcctcgcc
    17401 gggacgacgg gcgagatcgt cgagcggctc ggacatctct ccgaggccgg tgtgcagcga
    17461 gtaatgctgc agtggctgga ggtggacgac atagacgctc tcgaggccat ggcgcactcg
    17521 gtcctgccgc aactcagagg gtagtaagcg agtgcgctga ccctcgctga cattaacctc
    17581 ttatttctct tctgg
//
LOCUS       DATMVF010000311         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_43024, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000311 DATMVF010000000
VERSION     DATMVF010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43024"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1060)
                     /gene="gap"
                     /locus_tag="VN178_16290"
     CDS             complement(59..1060)
                     /gene="gap"
                     /locus_tag="VN178_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWS82580.1"
                     /translation="MAVRVGVNGFGRIGMLFTKAAMDRGDIEVVAANDLMPFDSLANL
                     FKHDSTHGIWPEDVSFDGNVLHVGDHEIKTFAERDPAQIPWGDVGADIVVESTGVFTS
                     RDGAAAHIEGGAKKVIISAPGKGVDATFVYKVNHEDYDPEAHEVVSNASCTTNCIIPM
                     VKVIQDNFGIESGYMTTVHAYTNDQSLLDAAHKDPRRARSAPNNIVPTSTGAARTAGV
                     IYPELQGKVDGMSMRVPVADGSITDFVARISSEASAEDVNAAFQSAADGELSGILQYS
                     EAPLVSSDIVGNAASCIFDSQLTMSNGGTVKVLGWYDNEWGYSNRTCDLAAYMGERL"
     gene            1537..2340
                     /locus_tag="VN178_16295"
     CDS             1537..2340
                     /locus_tag="VN178_16295"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HWS82581.1"
                     /translation="MTTSVVYGVLVVCGACLAAVLGLALVQRLVPTAIRQEQNDVAGF
                     IYAVVGVIYAVLLALVVIATWEEFGRARITVEAEANALAEIFWLAHRLPEPEGRELQE
                     LCQSYAEEVVNVGFPLMAQGRTPSLDRSQESSRAWVLIDDIRASLQEVEPRTLAGQEL
                     YAEGLDQVQRLADARRMRLVMAEESLPTVLWIVLIVGGIVAVGFAYLFGLQNTWAHAL
                     MVGSLAGVIALVLFTIAAMDHPFSGAARVGPEAFELVLARFETSKLSEL"
     gene            complement(2428..3324)
                     /gene="whiA"
                     /locus_tag="VN178_16300"
     CDS             complement(2428..3324)
                     /gene="whiA"
                     /locus_tag="VN178_16300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HWS82582.1"
                     /translation="MTLYCWRGRWARLPSFASEVRRQLAAGAASSARGVGRSELAGLV
                     DASGVWDEEGVTLRTPSAAVARSVVRLWRSEFGMESKLSPIDPDRFGRTRYAVRTAGN
                     GAIQAKEELRFARSARTAAERAGYLRGAFQGAASVNRPGGRGGHHLEFVHREAEFIRR
                     CADFSRAPLKIVRRRGRWIAYTKSADGVTTVLAQMGLNDAVLEYEARAILGEAKANAN
                     RVTNFDAANAGRTATAAARQHKALQAIDREHLPPALREMLELRLEYPDASLAELAHLG
                     NLSKSAANHRLRRLVSISARST"
     gene            complement(3269..>3598)
                     /locus_tag="VN178_16305"
     CDS             complement(3269..>3598)
                     /locus_tag="VN178_16305"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HWS82583.1"
                     /translation="GPVFYVANVMTQQGETSDFTVSDHVRAITAHVGPVVTDVLVHSG
                     DLSPEILARYEAEEAAPVAVDTEVLREMGLRVRETDLLSKESRAGVRHDPVLLAREVG
                     EAALVRL"
BASE COUNT          610 a         1100 c         1183 g          705 t
ORIGIN      
        1 cccaggccgc acacgaatgg tcttggctga tagctgacgg ctgaaagctg acagctatct
       61 acagtctttc gcccatatac gccgcgaggt cgcacgtacg gttcgagtag ccccactcgt
      121 tgtcgtacca gccgagtacc ttgacggtac cgccgttgga catggtgagc tgcgagtcga
      181 agatgcacga ggcggcgttg cctacgatgt cgctggagac cagtggggct tccgagtact
      241 ggagaatacc cgagagctcc ccgtcggcgg ctgattggaa ggcggcgttc acgtcctcag
      301 cggatgcttc gctggagata cgggccacga agtccgttat ggagccgtct gcgacgggga
      361 cgcgcatgga catcccgtcg accttgccct ggagctcggg gtagatcaca cccgcagtcc
      421 gcgccgctcc ggtggaggtc gggacgatgt tgttgggggc cgagcgggcg cgcctggggt
      481 ccttgtgggc cgcgtcgagg aggctctggt cgttggtgta ggcgtgcacg gtggtcatgt
      541 accccgactc gatgccgaag ttgtcctgga tcacctttac catcgggata atgcagttgg
      601 tcgtgcacga agcattggag acgacctcgt gggcctccgg gtcgtaatcc tcgtggttca
      661 ccttgtagac gaaggtggcg tcgacgccct tgcccggggc cgagatgatg accttcttgg
      721 ccccgccttc gatgtgggcc gcggcgccgt cgcggctcgt gaagacgccc gttgactcga
      781 ccacgatgtc ggcgcccacg tcgccccagg gtatctgggc cgggtcccgc tcggcgaagg
      841 tcttgatctc atggtcgccc acgtgcagga cgttgccgtc gaaggacacg tcctcgggcc
      901 agattccgtg ggtcgagtcg tgcttgaaaa ggttcgccag gctatcgaaa ggcatcaagt
      961 cgttcgccgc cacgacctcg atgtcgccac ggtccatcgc ggctttggtg aacagcatcc
     1021 cgatccggcc gaaaccattt acgcctactc ttacggccac tctactctcc attctctagc
     1081 ttgccgcata gtcttattct gcccgaaaag ccggaagatt atgcagcacg gtgccttcgg
     1141 cagcaaatca gcatttcagc ccgccgcatc cctacggtcg gcggctttca gcctttcggc
     1201 gggtcgtctc gatgcgtcgt cacgggcgtc aaacctcacc accacaagct gcgatctctc
     1261 gcgcggcggt ataaggctac agcgcagaaa catcgccgca acgcccgcgt aggtaggggt
     1321 agttggcctg tcagaaacgc cacctgctac tatccgaagt atcccgcgta ctacatccga
     1381 acttcgcgcg aacggagttc tgggaacctc gcctttctgc tgacgaggta agtccggttc
     1441 tccaggctcg cgtagaagct taggttgctt gtaccctctc cgggatgtat cttgggttcc
     1501 gcctgtgatc tctcttagac gaaagggaga agtcccatga cgacgagcgt agtttatggt
     1561 gttctggtgg tttgtggggc ttgcttggcc gcggtcctcg ggctggcgct ggtccagcgc
     1621 ctggtgccca cggcgatccg ccaggaacaa aacgacgtgg ccggcttcat ctacgccgtc
     1681 gtgggtgtaa tctacgctgt attgctggcg ctcgtggtca tcgccacgtg ggaagaattc
     1741 gggcgtgccc gcattactgt ggaagccgag gcaaacgcgc tggccgagat cttctggctc
     1801 gctcatcggc tccctgaacc cgaaggacga gagctacagg agctttgcca gtcctacgcg
     1861 gaagaggtgg tcaacgtagg gtttcccctg atggcgcagg ggcgaacgcc ttccctggat
     1921 cgttctcaag agagttcacg tgcgtgggtc ctcatcgacg acatccgggc gagcctccaa
     1981 gaggtcgagc ctcgcacgct ggctggccag gagctctacg cagaagggct cgaccaggtc
     2041 cagcggctgg ctgacgccag gaggatgcgc ctggtcatgg ccgaggagag cctaccaacc
     2101 gtgctgtgga ttgttctgat cgttgggggg attgtggcgg tgggcttcgc ttacctcttc
     2161 ggcttgcaga acacatgggc gcacgcgctg atggtaggat cgctagcggg ggtgatcgcg
     2221 ctggtgctgt tcaccattgc cgccatggac catcctttct ccggcgcggc ccgagtcggc
     2281 cccgaggcct tcgaactggt gttagcgagg ttcgagacga gcaagctcag cgaactctga
     2341 aaacatcggc gtgcgagccc ggggcagcaa agcaccatga ggaccggtgc tctcttgtgc
     2401 tggtcgacga gccgacagac ggaagagtca ggtggagcgt gctgaaatgc tgaccagccg
     2461 gcgtagccgg tggttcgccg cgctcttgga gaggtttccg aggtgggcga gttcggcgag
     2521 cgaggcatcg gggtactcga gccggagttc cagcatctca cggagggcgg gtggcagatg
     2581 ctcgcggtcg atagcctgga gggctttgtg ctggcgggct gcggccgtcg cagtgcggcc
     2641 ggcgttggcc gcgtcgaagt tggtgacgcg gttcgcgttg gccttggctt cgccaagaat
     2701 tgcccttgcc tcatactcga ggaccgcgtc gttgaggccc atctgggcga ggacagtcgt
     2761 tacgccgtcg gcgctcttgg tgtaggcgat ccaccgtccg cgccggcgta cgatcttcaa
     2821 gggagcacgg gagaaatccg cacagcggcg gatgaactct gcctcgcgat gcacgaactc
     2881 caggtggtgg cctccgcgac cgccgggacg gttcacggag gcggcgccct ggaacgcgcc
     2941 ccgcaggtag cctgctcgct ccgcggcggt gcgcgcggag cgggcgaacc tcagctcttc
     3001 cttcgcctgt atcgcaccgt tccccgccgt gcgcaccgca tagcgggtac ggccaaagcg
     3061 gtcggggtcg atcggtgaga gcttggattc cataccgaat tctgagcgcc acaggcggac
     3121 tacggagcgg gccactgctg cactgggagt gcgtagggta acaccttcct cgtcccagac
     3181 gccggaggcg tcgaccaggc cggcgagctc ggagcggccg acccctcgcg cactcgaagc
     3241 cgcgccagcg gcgagttggc gacgcacctc agaggcgaac gagggcagcc tcgcccacct
     3301 ccctcgccag cagtacaggg tcatgcctga cgcccgcgcg gctctccttc gagagcaagt
     3361 ccgtctccct tactctcaag cccatctcgc gcaagacttc cgtgtcgacg gcgaccggtg
     3421 ccgcctcttc ggcctcatat cgggcgagga tttcaggaga gagatctcca gaatggacga
     3481 gcacgtcagt gaccacgggc ccgacgtgcg ccgttatcgc ccgcacgtgg tccgagaccg
     3541 taaagtcaga cgtctcccct tgttgcgtca ttacgtttgc gacgtagaaa acgggacc
//
LOCUS       DATMVF010000312        12781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_274369, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000312 DATMVF010000000
VERSION     DATMVF010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12781
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274369"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VN178_16310"
     CDS             <1..1333
                     /locus_tag="VN178_16310"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWS82584.1"
                     /translation="VGKEEEAPVWDEEKNKALVRWWWEEVWVKGNVAAMDEFMAPNYV
                     DHPNLPGQPPGPEGMKQALSTYRTAFPELQATLDDIFAAGDRVALRWSARDTHLGEWL
                     GVPPTGHHFTMSGITIYRIAKGKAVEGWNSIEVNPTEEEQQWLTEGGGGWPRRSGSSD
                     DILPATEGDTSPPFWDVITHNLTWHFRVAEAQERERIEQELQFAWRIQQASLPKEVPE
                     PEGWQITPYYQPAREVGGDFYDFFELEDDRLGVVVGDATGKGVPAALVMTATSSMLRA
                     AARALGTSSPGEVLAHVNEALLARIPPNMFVTCFYAIVEPKSGRLRYANAGHDLPYLR
                     RRGGDDAEELRARGMPLGLMPETSYEEKVASLSEGESVLFYSDGLVEAHDPEGEMFGF
                     PRLRRLVAEYAAEEGSLVDFLMDELRSFTGDGWEQEDDVALVTLQRSAANR"
     gene            1498..1992
                     /locus_tag="VN178_16315"
     CDS             1498..1992
                     /locus_tag="VN178_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82585.1"
                     /translation="MVYPGATTNLGRSPRATSGSDPVIEEGVERVGAPRVLIAIEPRM
                     YAQVLAFLIGRHRPDAEVSLLGPSEELEDAALRLRPHLVVANRVPKAVREGPFFWVEQ
                     DEARGGEGARHLGARISADGYSESVEDVRIEHVLLALDHAERELVLESEGMNAPAEPR
                     VAQA"
     gene            2324..2617
                     /locus_tag="VN178_16320"
     CDS             2324..2617
                     /locus_tag="VN178_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82586.1"
                     /translation="MYAAVRRFEGVTDSQKVVQVAKEGFVPLISQMPGFVAYYLVDAG
                     DGVTVSTSVFEHKDAEEQSTFRAGEFVQEHLAPLMPNPPQVTAGEVVAYKVSS"
     gene            3001..4545
                     /locus_tag="VN178_16325"
     CDS             3001..4545
                     /locus_tag="VN178_16325"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWS82587.1"
                     /translation="MNGAESLVETLAGAGVEVCFANPGTSEMHFVAALDRVGGMRCVL
                     GLFEGVVTGAADGYARMADRPASTLLHLGPGLANGLANVHNARRANTPIVNIVGDHAT
                     YHKGYDAPLTSDVEGAARPFSDWVRMSADSRSVARDGADAVAAAMAPPGRVATLILPA
                     DASWNEADGPAPLPPVGGRAPVAPEAISRAARALRSGEDAVLFMGGRALREQGLELAG
                     RIAAMTGARLLAQTLNARVERGAGRVAVDLLPYPVDAALATLAGTRHLVLVGSEAPVA
                     FFAYPDKPSTLSPPQCETHTLATPGEDIVGALTALAEELGAAENTPVQAREEYTPSSG
                     EEITPQAAAAAVGTLLPEGAIVVDEALTAAAAFLSRTRGAAPHDLLQVTGGAIGIGLP
                     LATGAAVACPDRKVICLQADGSAMYTVQALWTQAREDLDVTTLILNNRSYAILKQELT
                     SVGQGDAGRGALDMMELDRPALDWVGLANSMGVEAGRARTMEELQRHLAAGLRSEGPY
                     LIDLVL"
     gene            4869..5975
                     /locus_tag="VN178_16330"
     CDS             4869..5975
                     /locus_tag="VN178_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82588.1"
                     /translation="MNRLYILAAVMVVTVGALFAGHQALAEVLTGTDDDNTLLGTDGE
                     DRLKGREGVDRIKGRDGDDLLKGNSGSDRLKGNSGDDHLWGGLGDDKITPGKGDDKVF
                     AGDGDDRIFVRAADGIDSIDCGAGFDKVRTTHSDDEAKKNCERAADSRQDDDGTTSTS
                     TSTTTTGTSTPRTTGTATDTTTGTTTGTGTLRTTGTTTSTTGTTTGTTSTTVTTPGTT
                     GTTSTSTTTGTTTGTTTGTTTGTASTNIVSNPTASASASASPTADTTTGTTGTTTSTS
                     TTGTTGTTGTSATDTADTASTTGADTTGTATTGATTTKGDTTPTKDDVIRDTIPEGRE
                     LPNTGGLSMLVPMGALLALLISGAAIGLSFVVRR"
     gene            6003..6926
                     /locus_tag="VN178_16335"
     CDS             6003..6926
                     /locus_tag="VN178_16335"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWS82589.1"
                     /translation="MFRGVPLLMRVGVIMMALALALVAAAAVVSAALRSEPERLVPAE
                     EVAAKSPGQAPRTSSGEGVSATEKSSGENEFSYYASAEEPLGQRKKEAKEPQAILQQQ
                     EAEPPDTLSAKPRPAGQQTPTGASQPLEHQPIRQERQVPISERKDWPEPTRGELESAN
                     RERRYDLLPGAIMGLTIEAMGIYDAPVFDSDGRWALANGVAHNPQTSLPWSPTPQRNV
                     YLAGHRMGFRGTWSRMIFYNLHKLNRGDEVLLKDRAGTSYRYRVSEVLVVDPTDLWVM
                     GQVRGRDMVTLQTCTPYPTFEKRLIVRADRV"
     gene            complement(7064..7477)
                     /locus_tag="VN178_16340"
     CDS             complement(7064..7477)
                     /locus_tag="VN178_16340"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWS82590.1"
                     /translation="MFDLHNETRTAYGLGPLCLSSVLTSAARVRSQDMLNRDYFSHYT
                     PGGVSVIDLLRRKGHYSSEPGDYHVLGENIAMGGDGYDEDTPEHLFTGLMQSEGHREN
                     ILRGEFEEVGLGARSGTYQEYDDISTIYTAVFGGR"
     gene            complement(8592..10010)
                     /locus_tag="VN178_16345"
     CDS             complement(8592..10010)
                     /locus_tag="VN178_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82591.1"
                     /translation="MERQKPIDEPHESSRAVTEQLAEYPLLEALIERRSRRFGNGMRL
                     NGGPLSYKSMHAPQPLSLGEEAALAFAGCGITGPVLAELPYDSGDEHEAGGGNIMINL
                     VGRTVASGDACHAVALFVINDEGAWMLKRPQDYPRTEIAELARAGRGHEFVRLYERSR
                     VRIADSRPDVPRELPFVMPFNKWAANVPGTTYLLPVNEFSAFYINVLLSAFSEDFGYF
                     LVDERNRFKPAGIARFARSKGGHLHDDPKEGRFTTVSFMETWLYEFAAVEQGEILQNL
                     ALMTEALGLGGFPHFAAHPFIWFRALGFRMQEIPFSHTIGAGALTKWLLKALGKELPI
                     PTAIGLERNGEALIKPFCPPYYRDMEEAVLAFVDRKYGRGTGAFRDGGDASSWQDGAG
                     VQAGIPPYSDRAIAATIAYCDYVYERYGRFPANSGPFRTVLAHQAHHLDPDFYDRFYN
                     PGTLTQTQRRHHHAAHHSGDGA"
     gene            complement(10127..12478)
                     /locus_tag="VN178_16350"
     CDS             complement(10127..12478)
                     /locus_tag="VN178_16350"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWS82592.1"
                     /translation="MSGGRDSSFGARLRKLRDAAGLTQEELASRSGLSAKNISDLERG
                     VRKRPYPHTVRSLADALKLSEEERAALIAAVPKRGGTPAAAPEEPTLPVPPTLLVGRE
                     RELEEVADLLRRPEVRPLTLTGVGGVGKTRLALEAARNGAGDFPDGVNFVGLASLTEV
                     TLVVPTICQTLELRKVGQRSMHAALQEHLREKELLLVLDNFEHVLEAACEIAELIEMC
                     PSLTVLATSRAPLRLRGEQRYPVSPLGLPASTRFPAAKEVVGSPSGRLFVERARATSP
                     TFELEAANAAAVASICWRLSGLPLALELAAARVGFLSPPSLLARLDQALSAGWARDVT
                     ERQRTMSATLDWSYDLFAESEKALFRRLSVFAGGFSLEAAEAAGPAEDDDAGNVFHLL
                     GELVGQSLVAVETLPDETADRPRYGMLEPVRQYANEKLEECGESAAASRRHAEFFLDL
                     AERAYPELRGERQGEWLEQLEQENGNLRAAVSWAVSAREAETASRLGWALWLFWWLRG
                     YQREGRRWMEMLLEQDSPAALRAIDSAVAGIMAYTQGDYEASRGHLEEGLELARSVGD
                     TLRTAYATYALGLLALNTQEYPTARARLEEALDLFLERGDDHDAATVRSHLGTLLLVQ
                     GDYDKATAPIEEGLALARKLGDRFGISDALYNLAQVAQARGDNDLAALRFEEGMTLSA
                     EMEDRANPGYFLEGLAEVAGVRDEAERSARLFGAAERMLRAAEAPVYEVYEPNRAFYE
                     RVKDDVRLRLGKGAYEALQAEGQTMTFERAVAYALKHDQASSA"
BASE COUNT         2411 a         4066 c         4049 g         2255 t
ORIGIN      
        1 cgttggcaag gaggaggaag ctcctgtgtg ggacgaagag aagaacaagg ccctggtccg
       61 ctggtggtgg gaggaggtgt gggtcaaggg caacgtggcc gccatggatg agttcatggc
      121 tcccaactac gtagatcatc ccaaccttcc tgggcaacca cctggccccg agggcatgaa
      181 gcaggcgctc agcacctacc gcaccgcctt ccccgagttg caggccaccc tcgatgacat
      241 cttcgccgca ggtgacaggg tggcgctccg ctggagcgct cgcgacaccc acctgggcga
      301 gtggttaggc gtccccccaa ctggacacca ctttacgatg agcggtatca ccatctaccg
      361 gatcgccaag ggcaaggcgg tggaaggctg gaacagcatc gaggtgaacc ccacggagga
      421 agagcagcag tggttgaccg agggtggcgg tggatggcct aggaggagcg gcagcagcga
      481 cgacatcctc cccgccaccg agggcgatac gtctcccccg ttctgggatg tcataaccca
      541 caatctcacc tggcacttcc gggttgccga agcccaggag cgggaacgca tcgagcaaga
      601 gctgcaattc gcatggcgca tccagcaggc ctcgctcccc aaggaggtgc ccgaaccgga
      661 gggttggcag atcactccct actatcagcc cgccagggag gtaggaggag acttctatga
      721 cttcttcgag ctagaggacg accggctggg agtagtcgtg ggagacgcca cgggcaaggg
      781 cgtcccggca gccctggtga tgaccgcaac ctcctccatg ctcagggcgg cggcccgagc
      841 gctgggtacc tcctcaccgg gggaggtgct ggcccacgtc aacgaggcgt tgttggctcg
      901 tatccctccc aacatgttcg tcacctgctt ctacgccatc gtagagccca agagcggacg
      961 cctcaggtat gcaaacgccg gccacgacct gccttaccta cgtcgccgtg gtggagacga
     1021 cgccgaggag ctcagggcga gagggatgcc cctggggctg atgccagaga cgagctatga
     1081 ggagaaggtt gcctctctga gcgagggaga gagcgtcctc ttctacagcg acgggctggt
     1141 ggaggcccac gatcccgaag gcgagatgtt cggcttcccg aggcttagga ggctggtcgc
     1201 ggagtatgct gctgaagaag gatcgctggt tgacttcttg atggacgagc ttcgctcctt
     1261 caccggggat ggctgggagc aggaggacga cgtcgctttg gtgacgctac aacgttcggc
     1321 tgcgaaccgc tgaacgtccg agaaatctag cccatttggg ggatgcgccc ttggtggtga
     1381 gggtgctacg gtaccctcgc gtacgtacag ctgaaggggg agagacgagg aagctccgcc
     1441 ctttcgggac agagtgcgta ttttcttgag gcaaccgacc acgagggggt tcctggcatg
     1501 gtttatccag gtgctactac gaaccttggg cgatctccgc gcgctacttc cggctcggac
     1561 cctgttatcg aggaaggagt cgagagagtc ggcgcgccgc gagtcctgat agccatagag
     1621 ccgcgcatgt acgcgcaggt gctcgccttc ttgatcggca ggcatcgccc cgatgccgag
     1681 gtctcgctgc ttggtccttc cgaggagctg gaggacgcgg cgctccgctt gcgcccgcac
     1741 ctggtggtgg ccaaccgggt accgaaggcg gtgagggagg ggcccttctt ctgggtagag
     1801 caggacgaag cgcgcggggg cgagggagcc aggcacctcg gggcaaggat aagcgccgac
     1861 ggttattccg aaagcgtgga agacgtgaga atcgagcacg tgcttttggc cctcgaccac
     1921 gccgagcggg agctcgtcct cgagagcgaa ggcatgaacg ctcccgccga gccgcgggtg
     1981 gcccaagcct gatggcccaa accgagtttc ctggctctat tcatgggagg tgacagcccg
     2041 ttgaaaagcc tctctacgtc gcttccgggt ctccttcacc ggctcgaaaa tcctccatcg
     2101 cagagttttg gccctgatat ccgacggttt ggatgcggtt ttggagtacc cacctgtcct
     2161 atgtcgactt tttcgaaagg ctggtgaatt ctccgaactt cgactttcac gatttctacg
     2221 acgttgcgct agccttcgta acccctcata cttaccttcg ttgatcaact aaggcggggg
     2281 cgacgggtct cgccctccct caaggaggag gtagcataaa gcgatgtacg ctgcagtgcg
     2341 gcgcttcgag ggcgtgaccg actcgcaaaa ggtggtgcaa gtcgccaagg aaggctttgt
     2401 gcccctcatc agccaaatgc ctggattcgt ggcttactac ttggtcgacg ccggggacgg
     2461 cgtaacagtc tctaccagcg tcttcgagca taaggacgct gaggagcagt cgacctttag
     2521 ggcaggagag ttcgtgcagg agcatctagc tcccctgatg cctaacccac cccaagtcac
     2581 agccggtgaa gttgtcgcat acaaggtgtc gtcataggcg agagtcagag ttctagagct
     2641 tgacgtgaga gagccgggac tctcaggagg cctggctttc tctttgccct tattccccgg
     2701 aattcctgga agcttcacct tgggtgtgga aggtgccggc ctcgccgacg gcttgcggga
     2761 ggcactggcc gccggaagtg ctcccgcgcc gccactgtcc tcgattgaac cagcggctac
     2821 ttgggtgcca catgcttgca ctttgggtgc tatcctgcgg gcggcctggg gaaccgttct
     2881 gaaaggtagg atactcgcgt tggaagacgt cagtggtagc ggcccatacc caacgacacc
     2941 acggtttcgt cgagactaag attggtgggg tgtgtttatc gataggagcg taggcgagga
     3001 atgaacgggg cggaaagctt ggtcgagacg ttggccggcg cgggagtgga ggtctgcttc
     3061 gccaacccgg gcacctcgga gatgcacttc gtcgcggccc tagatcgggt cgggggcatg
     3121 cggtgcgtgc tcgggctgtt cgagggcgtc gtgacgggcg ctgccgacgg ctatgcgcgc
     3181 atggcggaca ggccggcttc gacgcttctg catctggggc ccggactcgc caacgggctc
     3241 gccaacgtgc acaacgccag gcgcgccaac acgccgatag tcaacatcgt cggcgaccac
     3301 gcgacctacc acaaagggta cgacgcaccg ctcacctcgg acgtcgaggg tgcggcgagg
     3361 cccttctcgg actgggtcag gatgtcggcc gatagccgct cggtcgcgcg cgatggcgcg
     3421 gatgccgtgg cagcggcgat ggccccgccg gggcgggtag cgaccctgat cctcccggcc
     3481 gacgcctcct ggaatgaggc ggacgggccg gcacctctgc cccccgttgg gggtcgcgcg
     3541 ccggtagcac ccgaggcgat ctcccgtgct gccagggcct tgcgctccgg cgaggacgcg
     3601 gtcttgttta tgggcggccg tgccctgcgc gaacagggcc tcgagcttgc cggtcgcatc
     3661 gccgcgatga ccggagcgcg tctgctcgcg cagacgctaa acgcgcgggt ggaacgaggg
     3721 gcggggcggg tcgccgtcga tctcctcccc tacccggtcg acgccgccct cgccaccctc
     3781 gccgggacgc gccacctcgt gctggtcggc tcagaggcgc cggtcgcctt cttcgcttac
     3841 cccgacaagc cgagcacgct gagcccgccg caatgcgaaa cccacacgct cgccacgccg
     3901 ggcgaggaca tcgtgggggc gcttacggcg ctcgccgaag agctcggagc ggctgagaat
     3961 acccccgtcc aggcgcgaga agagtacaca ccatcctcgg gtgaagagat cacgcctcag
     4021 gcggccgccg cagcggtagg gacgctcctt ccggaaggcg cgatcgtggt cgacgaggcg
     4081 ctcaccgccg ccgcggcctt cctgtcgcgg acgaggggcg cagccccgca cgacttattg
     4141 caggttaccg ggggcgccat cggtatagga ctgccgctcg ccaccggggc ggcggtcgca
     4201 tgcccggacc gaaaggtgat ctgcctgcag gccgacggca gcgcgatgta caccgtgcag
     4261 gccctgtgga cccaggcgcg cgaggacctc gacgttacca cgctcatcct gaacaaccgt
     4321 tcctacgcga tcctcaaaca ggagctgacg agcgtcggcc agggcgatgc cgggcgcggg
     4381 gcgctagaca tgatggagct cgaccggcct gcgctcgact gggtaggcct cgccaacagc
     4441 atgggcgtgg aggccggacg cgccaggacg atggaggagc tccagcgcca tctcgccgcc
     4501 ggactgcgct ccgagggtcc gtatctgatc gacctcgtgc tgtaacggct tgcaccaaca
     4561 gccatccacc cggtttgagg gcggcggcca tccccgcgag aacagccgga gcgtactcgg
     4621 agatctggtg cagtaaggcg cggcaccacc cgagaccagc gaacaatatt ccacgaactt
     4681 cgctttgacg gggttctcgg aaggcttttg aggttgcgct atgccccgaa gctgttattg
     4741 ttccggctgt gacgaaggga accgccatcc gtggtggggt agagaccaag aacgagagta
     4801 tctgttgata atgtccaggg agaaggggag cggcacaagc tccacggtag actaaggcag
     4861 ggaatcgaat gaatcgactg tacatcttgg ccgcagtgat ggtcgtgacg gtcggagcgc
     4921 tcttcgccgg gcaccaagcc ctggcggagg tcctcaccgg caccgacgac gacaatacgc
     4981 tcctcgggac cgacggagag gaccgcctca aaggtcgcga aggggtggat cgcatcaagg
     5041 gtcgcgacgg ggatgacctg ctcaagggca acagtgggtc cgaccgcctc aagggcaaca
     5101 gtggcgacga tcacctttgg ggtggcctgg gcgacgataa gatcacgccc ggcaaaggcg
     5161 acgataaagt gttcgccggc gacggagacg accgtatctt cgtgcgcgcc gccgacggca
     5221 tagattctat agattgcggg gccggattcg acaaggtccg gaccacgcac agcgacgatg
     5281 aagccaagaa aaactgcgag agagccgccg actctcgcca ggatgatgac ggaactacca
     5341 gcacaagcac cagcaccacc accaccggca ccagtacccc acgcacgacg ggcaccgcta
     5401 cagatacgac aacgggaacc accacaggca ctggcaccct gcgcacgacg ggtacgacca
     5461 ccagcacgac aggcacgaca acaggaacta cctcaaccac tgtcaccact ccaggtacga
     5521 caggaacgac gagtacgtct accactacag gtacgacaac gggaaccacc acaggcacga
     5581 caacgggtac tgccagcacc aacatagtct cgaacccgac agcgagcgcg agcgcaagcg
     5641 caagcccgac cgcagatacg acaacgggaa ccaccggcac caccaccagc acaagcacca
     5701 ccggcaccac tggcactacc ggcacgagcg ccacggatac tgcggacacc gcgagcacca
     5761 cgggcgcgga taccacgggc acggcgacca ccggtgcaac taccaccaag ggcgatacga
     5821 cccccactaa ggacgacgtg atccgtgaca cgatccccga gggcagagaa ctacccaaca
     5881 cgggtggcct ctccatgctc gttccgatgg gcgcactgct tgcgttgctc atcagcgggg
     5941 cggccattgg actgtcgttt gtagtaaggc gctaacactc tacatacgag gaaaggtagc
     6001 gggtgtttag aggagtcccg cttctcatga gggtgggtgt gatcatgatg gcgctcgcac
     6061 tcgccctcgt ggccgccgca gccgtggtga gcgccgctct acgcagcgag cccgaacggt
     6121 tggtcccggc agaggaggtt gccgccaagt cccctggaca agcgccgcgc acctcctcag
     6181 gtgaaggagt gagtgcgacc gagaagtcat ctggcgagaa tgagttctcg tattacgcct
     6241 ctgccgagga gccgttgggg cagcgcaaga aggaagccaa ggagccgcag gcgatactcc
     6301 agcagcagga agccgaaccc ccggacactc tgtccgcgaa gcctcgaccg gcgggccagc
     6361 agacacctac tggagcgtcc caaccactcg agcaccaacc catccgacaa gagcgacagg
     6421 tgccaatctc tgaacgaaag gactggcccg agcccacccg aggagagcta gagagcgcca
     6481 accgagaacg ccgctacgat ctgctacccg gagccataat gggcctcacc atagaagcga
     6541 tgggcatcta cgacgccccc gtcttcgact ccgatggccg ctgggcgctt gccaacgggg
     6601 tcgcccacaa tccgcagact tccctgccgt ggtcacccac tccccagagg aacgtctacc
     6661 tggcgggaca ccgcatgggc tttcggggaa cctggagccg catgatcttc tacaacctcc
     6721 ataaactgaa caggggggac gaagtactcc taaaggaccg ggccggcacc tcctacagat
     6781 accgggtaag cgaggtattg gtagtcgatc ccaccgactt atgggtgatg gggcaggtga
     6841 gggggaggga catggtgacg ttgcagacgt gcacccccta tcctactttc gagaagcgcc
     6901 tcatagtgcg tgccgaccga gtctaatcgc cacatttgcg catcgctgca aagccccgag
     6961 cattccgaac atcacgtatc ggtaggggag gggtgcagcc cttgtcccgg ctgatagctg
     7021 aaggctttac tctcggaaag gttcaccctt tccgagagtg gccctaacgc cccccgaaca
     7081 ccgcggtgta gatcgtcgag atgtcgtcgt actcctggta ggtgccagat ctcgccccca
     7141 agcccacctc ttcgaactcc cctctcaata tgttctccct gtggccctcg ctttgcatga
     7201 ggcctgtgaa aaggtgctcg ggggtatcct cgtcgtagcc atcgcctccc atggcgatgt
     7261 tctcccccaa cacgtggtag tcgcccggtt cggaactgta atgaccctta cgcctgagca
     7321 gatctataac cgacacacca ccaggcgtgt agtgtgagaa gtagtctctg ttcaacatgt
     7381 cttgtgagcg gacgcgggcc gcactcgtga gaacggagga tagacacaag ggcccgagtc
     7441 cataggccgt cctcgtctca ttgtgtagat cgaacatctc tttctccgcg acgttcaggg
     7501 cgagggtgcc gccatcacaa gtccgcacat tcaccaatgt agacgtatcg gccggcggtt
     7561 cgtctccatg tatccaggac ccgccgctct gtggagccgg tttcgtggtg tatggggttg
     7621 gcgtctgcac actcagcggc cgggttgcgc tgggcgactg ggctaccggc cctacctggg
     7681 gcgtcgcgtc gatcggccga gcgaggcgaa cgcctacagg agcatcgacg cgtgatgtct
     7741 gaccttgccc atcatgacgc atcgcacttg ccgccacggc ccccgtgaag gacagcgcga
     7801 cgaagagaag caccacgccg gccaccaaaa gcgcaccccc acgcaaccgg atcatgcccc
     7861 ctcgagcgcc gatttcgcca gatctacgac actgtgcata aagattggcc cctttttcat
     7921 cactagctat cagcgcccga ctctctcagc attcagctct tgtcctggct gaaagccgat
     7981 ggctgacggc tccttttcgg aaaggttcat cctattcgag aaaggctcct ggcacatact
     8041 agcgcacgcg actgccacgc gctagctacc gcctcagtat ctacgtgtgc agaatcgagg
     8101 cggtggcgca gggcagcgtc tccgaggtca gagagccggt gggctccctt cgacgctgaa
     8161 cgctcgcact ttgtcctgat acgtcggcga gatcgacact gcataagagt gggctggcgt
     8221 ggcgcgcaaa ccagcgctcc acgccggggc ctccttcatg gaggatacac ctgcccgcat
     8281 cgaggcgagt cttaacgaga ctgaagcgga ccctaacaac gtttaaggct cgaggtgata
     8341 gattcggtgg tccgtagtcc ggtcggggct tcccccctcc gtcctggccg gacttctgca
     8401 cgaactcggc cggggctctg cggcgctccg gcgtttcgtg cgccgcactg taaccgctcc
     8461 tgcctggcag ccttgggccg gttggaattg gacgaggcga acttctacat tcatccggtc
     8521 cgtcgctgac ctgttgcggg gtgattacaa acggtcccga tacgaaaggt gatcctgccc
     8581 tgaaacgcga atcatgcgcc gtcgccgctg tggtgggccg cgtgatgatg gcggcgttgg
     8641 gtttgtgtca acgtcccggg attgtagaac cggtcgtaga agtccgggtc cagatggtgg
     8701 gcctggtgcg cgagcaccgt acgaaacgga ccgctattgg ccggaaatcg cccgtacctc
     8761 tcgtacacgt agtcgcagta tgcgatcgtg gcggcgatgg cacgatccga gtaaggagga
     8821 atgcccgcct gaacaccggc tccatcctgc cagctcgagg cgtcgccccc gtcgcgaaat
     8881 gcgccggttc cccggccata cttacggtct acgaaagcga gcacggcctc ttccatatca
     8941 cggtagtaag gtgggcagaa gggtttgatc agagcctccc cgttccgctc tagtcctatc
     9001 gcggtaggga tgggcaactc tttgcccaat gccttcagca accacttggt cagcgcgccg
     9061 gcgccgatag tgtgggagaa ggggatctcc tgcatccgga acccgagggc ccggaaccag
     9121 atgaaaggat gcgctgcgaa gtgcgggaat ccgcccaagc cgagggcttc ggtcatcagc
     9181 gccaggttct gcagtatctc gccctgctct accgccgcga actcatagag ccaggtctcc
     9241 atgaaggaca cggtggtgaa gcgaccctct ttggggtcgt cgtgcaggtg cccgcccttg
     9301 gatcgcgcga atctcgcgat gccagcgggc ttgaagcggt tgcgctcgtc caccaggaag
     9361 tagccgaagt cctcgctgaa cgcggagagc aaaacgttga tgtagaaagc agagaactcg
     9421 ttgaccggca ggaggtaggt agtgccgggt acgttggccg cccacttgtt gaacggcatc
     9481 acgaaaggca gctcgcgtgg cacgtcaggt cggctgtcgg cgatgcgaac tcggctcctc
     9541 tcgtagagcc gcacgaactc gtggcctcgg ccagctcgag caagctccgc gatctcggtc
     9601 cttggatagt cctggggtcg tttgagcatc caggcccctt cgtcgttgat gacgaagagg
     9661 gctacggcgt gacaagcgtc gccgctcgcc accgtacggc cgacgaggtt gatcatgatg
     9721 ttgccaccgc ccgcttcgtg ctcatcgcca ctgtcgtagg gcagttcggc caacacgggg
     9781 ccagtgatgc cgcagcctgc gaatgccaag gcggcttctt ccccgaggct cagcggctgc
     9841 ggagcgtgca tactcttgta ggagagcgga ccaccgttca ggcgcatgcc attgccgaag
     9901 cgtcgtgagc ggcgttcgat cagggcttcc aacaacgggt attcggccag ctgttctgtc
     9961 accgcccggg atgattcatg tggttcgtct atcggcttct gccgttccat ttcgctctcc
    10021 ttcccacccg ttcaggtacc gacgaggcac ccctatccac cagacacatg atgccatcga
    10081 ccgagacgcg ttgtgtcagc ggaaggcccg tcagccctga cactgcttat gcggaagagg
    10141 cctgatcgtg cttgagtgcg tacgccaccg ctctctcgaa tgtcatcgtc tggccttcgg
    10201 cctgtaatgc ttcgtatgct cctttcccga gccgcaagcg cacgtcgtct ttgacgcgct
    10261 cgtagaatgc gcgattcggc tcgtagacct cgtaaacggg cgcttcagcc gcccgtagca
    10321 tccgttctgc tgcgccgaac agacgcgccg agcgctccgc ctcgtcccgc acgccggcca
    10381 cttcggctag cccttccagg aagtagccgg ggttggcccg gtcctccatc tctgcggaga
    10441 gcgtcatccc ttcctcgaac ctgagggccg ccaggtcgtt gtctccgcgg gcttgcgcga
    10501 cctgggcgag gttgtagagc gcgtcgctga tgccgaacct gtcgcccaac ttgcgggcga
    10561 gcgcgaggcc ctcttcgatc ggcgcagttg ccttatcgta gtcgccctgg acgagcagta
    10621 gcgtgccaag gtggctacgc acggtggcag catcatgatc gtcgcctctc tcgaggaaga
    10681 gatccagggc ctcctccagt cgcgccctcg cggtcgggta ctcctgggta ttcagggcca
    10741 ggaggccgag cgcgtacgtc gcgtaggcag tgcgtagggt gtcgcctacg ctccgggcca
    10801 actccaggcc ctcctcgagg tgaccccggc tcgcctcgta gtcgccctgc gtgtatgcca
    10861 tgatgccagc caccgccgag tcgatcgcgc gtaaggctgc tggactgtcc tgctccagaa
    10921 gcatctccat ccacctgcgt ccctcccgtt ggtagccacg aagccaccag aagagccaca
    10981 gcgcccaccc caggcgcgag gcggtttcgg cctctcttgc agagaccgcc cagctaacgg
    11041 cggcacgcag gttgccgttc tcctgctcca actgctccag ccactccccc tgacgctctc
    11101 cacgcaactc cgggtaagcc cgctcggcca gatcgaggaa gaattcggcg tggcggcggc
    11161 tggccgccgc gctctcgccg cactcctcca gcttctcgtt cgcatactgc ctgaccggct
    11221 cgagcatccc gtagcgcggc cgatcggcgg tctcatccgg caaagtctcg accgccacca
    11281 atgactggcc gaccagctct ccgaggagat ggaacacgtt accggcatcg tcgtcttccg
    11341 ccgggcccgc agcctccgcc gcctccagtg agaatccacc ggcgaagacc gacagccgtc
    11401 tgaataacgc cttctcggac tcggcgaaca ggtcgtagct ccagtccagc gtcgcgctca
    11461 tggtccgctg acgctccgta acgtcccgcg cccagccggc tgacagggct tgatccaggc
    11521 gcgccagcag ggacggtggg cttaggaacc cgactctggc agccgccaac tccagcgcga
    11581 gcggcagtcc tgagagccgc cagcagatgg acgctaccgc agccgcgttc gcagcctcga
    11641 gttcgaaggt tggggaagta gctcgggcgc gctctacgaa cagacgtccc gagggagacc
    11701 ctaccacctc cttggctgca ggaaagcggg tggaggccgg caatcccagg ggagagacgg
    11761 gatacctttg ctcgcccctg aggcggaggg gcgcgcggct ggttgcgagt actgtgaggc
    11821 ttggacacat ttcgatcagc tcggcgatct cgcaggccgc ctcgagcacg tgctcgaagt
    11881 tgtccagtac gagcagtagc tccttctccc gcaggtgttc ctgaagggcg gcatgcatcg
    11941 accgctggcc gaccttcctc agttccaagg tctggcagat ggtgggcact acgagcgtaa
    12001 cttcggtcag ggatgccaat ccgacgaaat tcacaccgtc agggaagtct ccggcgccat
    12061 tacgtgccgc ttccagggcg aggcgcgtct ttccgacccc gcctaccccc gtgagcgtca
    12121 gcggcctgac ctccggccgc cggagcagat cagccacctc ctccagctct cgctctcgcc
    12181 ccacgagcag ggtgggcggc accggaagag tgggctcctc gggagcagcc gccggggtgc
    12241 cgccccgctt gggtaccgct gcaatgaggg ctgcgcgctc ctcctcggac aacttcaggg
    12301 cgtcggccag ggacctgacg gtgtgcgggt aggggcgctt ccttacgccg cgctccaggt
    12361 cgcttatgtt cttggcagac agccccgatc tggaggcaag ctcctcctgc gtgaggccag
    12421 cggcgtccct gagctttctg agccgtgcgc cgaacgagga atcgcgcccc ccgctcacac
    12481 cgaacttccg tccctcgtca tcgacctgcg tgcctttccc tgatctccag gcatggtagc
    12541 agacggtaca agggatcgac gagtattgcc gttactcgcg tacttcgact gcgggagtaa
    12601 tgcgggagta gtgcggatgg tctagagccg cggcggggcg cagactagat gtgtgttgtc
    12661 aggatcatgc ccacagctcc ttgatgcgcc cttgaggacg tcccagcttc cggttgacca
    12721 cgaaggcata tgtgtaggct gctatcttgg ccgcgatcct ggtggccagc ccagtgagcg
    12781 t
//
LOCUS       DATMVF010000313         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_45261, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000313 DATMVF010000000
VERSION     DATMVF010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45261"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..764)
                     /locus_tag="VN178_16355"
     CDS             complement(30..764)
                     /locus_tag="VN178_16355"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HWS82593.1"
                     /translation="MKPVKPAKGQQGVARSFGHAYRGLVYAVRTQRNMRFHVIAAAVV
                     LVLSLLVGVSKLELAILVLAITMVFGTEMVNTALEFAVDLATREYHPLAKLAKDVSAG
                     AVLVTSVAALAVGYLVLSDDLGPFSLETLERVRRWPGHVTLVALVLVAIAVILVKALT
                     RAPNPFLGGMPSGHAAVAFAGWVAASFVAVEGAVRFAGLISLITLLMALLVCQSRIEA
                     GIHTVYQVIAGAILGTLLAVAVFQLF"
     gene            complement(751..1197)
                     /gene="ybeY"
                     /locus_tag="VN178_16360"
     CDS             complement(751..1197)
                     /gene="ybeY"
                     /locus_tag="VN178_16360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWS82594.1"
                     /translation="MLFFFFFVAVLDETEYEKLSAERATELCAAAFSFRGFDVDRMGE
                     VSVALVPLPVIHELNLKYRDIDTPTDVLSFGIDGPYGEMVGEVVLAPEYVRFERAGVE
                     ELVVHGALHLAGMDHGDDFARSEMAAVQEAVLRSVREGHGADRETG"
     gene            complement(1205..>2681)
                     /locus_tag="VN178_16365"
     CDS             complement(1205..>2681)
                     /locus_tag="VN178_16365"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWS82595.1"
                     /translation="KAEEFHRPPSRTERIRVWLEGLPRVRLYASLLLIVWLSLTFLIG
                     FDVRPQVSDGPSSWTAFLGVGIVVATEAWIARYFLERFGQRILRSNALIRVLLAALLL
                     VLFTALARAFILFSLPPYLIPLAGLSILGTILLGPRLMFLMVVITSVNVGILAGNDFS
                     LTASLLLASGFAIYTVVLRVGPRMELLRAGLLIALVSGIVTFAVALVEGNVLRTAVER
                     GGIGLVNGILSLMLAMVLLPLLESTFNILTPMKLLELSDPGNPLLQKLLRGAPGTFSH
                     SMQVGNLAENAAERIGADPLLARVGAYYHDIGKLEHPNYFIENQISRMNPHETLAPAL
                     SARIVKRHVKDGVELGREWNLPAEVLDLIRQHHGTTRIGYFYRKALDEAATNGATINE
                     SDFRYPGPCPMSKEAGILMLADSIEATVKSLEKPTPKRIEDIVDGTIRAKLEDGQFDQ
                     CELTMREIHETGEAIREALIGFIGPRIEYPPEAPAAKMPTS"
BASE COUNT          544 a          831 c          867 g          439 t
ORIGIN      
        1 atccacgacc tcctaattat aggagcccac tagaagagtt gaaacacggc gacggcgagg
       61 agggtaccga ggatcgcgcc ggcgatgacc tgatagacgg tgtggatgcc ggcctcgatg
      121 cggctctggc agacgagcag ggccatgagc agcgtgatca gggagatcaa accggcgaac
      181 cgtacggccc cttcgaccgc tacgaagctc gccgctaccc acccggcgaa cgcgaccgcc
      241 gcgtgcccgg agggcatccc gcccaagaac gggttcggcg ctcgcgtgag cgccttcacg
      301 agtatcacgg cgatggccac gagcacgagc gcgaccagcg taacgtgtcc gggccagcgg
      361 cgcacacgct ctagcgtctc cagcgagaag ggaccgaggt cgtcgctgag gaccaggtac
      421 cccaccgcca aagccgcgac gctcgtcacg agcacagccc ccgccgaaac atctttggcg
      481 agcttggcca aaggatggta ctcgcgggtc gcgaggtcca cggcgaactc aagcgccgtg
      541 ttgaccatct ccgtgccgaa gaccatcgtg atcgccagta ccaggatcgc cagctctagc
      601 ttgctcacac ctacgagaag gctcagcacc aggacgaccg cagctgctat gacgtgaaaa
      661 cgcatgttgc gctgggtgcg tactgcatag accaaccccc tgtacgcgtg cccgaaggag
      721 cgcgctaccc cctgctgccc cttcgcaggc ttaaccggtt tcacggtctg ccccgtgtcc
      781 ctcgcgaacg ctcctcagca cggcctcctg caccgcagcc atctcgctcc tcgcgaaatc
      841 atccccgtga tccatccccg ctaggtgtag cgccccgtga accaccaact cctcgacgcc
      901 cgcccgttcg aaacgaacgt actccggcgc caacacgacc tcaccaacca tctcaccgta
      961 cggcccgtcg ataccaaagc tcaacacatc ggtcggggta tctatatccc tatacttaag
     1021 gtttaattcg tgaatgacgg gcaggggcac gagggcaacc gaaacctctc ccatacggtc
     1081 aacatcgaag ccccgaaacg agaaggcggc ggcgcacagt tcggtggccc tttcggcgct
     1141 aagcttttcg tattccgttt cgtccaggac ggcgacgaag aagaagaaga agagcaaggg
     1201 gtctctagga ggtcggcatc ttggcggcgg gggcttcggg agggtattcg atgcgaggac
     1261 ctatgaatcc tatgagggcc tcgcggatgg cctcgccggt ttcgtggatc tcacgcatcg
     1321 tcagctcaca ctggtcgaac tgtccgtcct cgagcttggc ccggatggta ccgtccacga
     1381 tgtcttcgat acgtttgggg gtcggttttt cgagcgattt gacggtggcc tcgatagagt
     1441 cggcgagcat caggatcccg gcttccttgc tcatggggca ggggccgggg tagcggaagt
     1501 ccgactcgtt gatggtcgcg ccgttcgtgg cggcctcgtc gagagccttg cggtagaagt
     1561 agccgatgcg ggtcgtgccg tggtgctgcc ttattaggtc gagaacctcg gcaggcaggt
     1621 tccactcgcg cccgagctcg acgccgtcct tgacgtggcg cttgacgatc cgggccgaga
     1681 gggcgggggc aagggtctcg tgggggttca tgcgtgatat ctggttctcg atgaagtaat
     1741 tggggtgttc gagcttgccg atatcgtggt agtaggcccc gacgcgggcg agcagggggt
     1801 cggccccgat acgttcggcg gcattctcgg cgagattgcc gacctgcatg gagtgggaga
     1861 aggttccggg cgccccgcga aggagcttct gcaggagcgg gttgccgggg tcggagagct
     1921 ccaggagctt catcggcgtg aggatgttga aggtgctctc gaggagcggg agtaggacca
     1981 tggcgagcat caacgagagg ataccgttca caagcccgat accgccccgt tccacggccg
     2041 tccgaagaac attgccctcg acgagagcga ccgcgaaggt cactattccc gagacgaggg
     2101 cgatgagcaa ccctgctcgc aagagctcca tccgaggtcc cacccgcagg acgaccgtgt
     2161 agatcgcgaa ccccgaggcc aggagcaggg aggcggtcaa ggagaagtcg ttgccggcga
     2221 ggatgccgac gttcacgctg gttatgacga ccatgaggaa catcaggcgc ggccccagga
     2281 ggatggtgcc caagatgctc aggccggcca gcgggataag gtagggaggc aaagagaaca
     2341 ggatgaaggc ccgggcgagc gcggtgaaga gcaccaggag caaggccgcg agcaggacgc
     2401 ggatgagggc gttgctgcgc aggatcctct gtccgaaccg ctcgaggaag taccgggcga
     2461 tccacgcctc ggtcgccacc acgatcccta ccccgagaaa cgccgtccag gagctcgggc
     2521 cgtccgagac ctggggacgg acgtcaaagc ctatcaggaa cgtcagcgag agccagacga
     2581 tgaggagcaa cgacgcgtac aaccgtaccc tcggcagccc ctcgagccag accctaatcc
     2641 tctcagtccg agacggcggc cggtgaaatt cctccgcctt c
//
LOCUS       DATMVF010000314        21322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13061 k141_45394, whole
            genome shotgun sequence.
ACCESSION   DATMVF010000314 DATMVF010000000
VERSION     DATMVF010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943658
            Sequence Read Archive: SRR7497548
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 21322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:45:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,265
            Genes (coding)                    :: 3,249
            CDSs (with protein)               :: 3,249
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21322
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45394"
                     /isolate="SMAG_U13061"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.31 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VN178_16370"
     CDS             <1..309
                     /locus_tag="VN178_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82596.1"
                     /translation="AETIKHAPHFGNEETVSAELEERVRAYFGLEPLLSPGDPHPPDD
                     VGRFGPDDRVDTEPGERMEAERRQGGLAGDPVAGGRIPEDRWDRETTADDVTIHRRRR
                     "
     gene            380..919
                     /locus_tag="VN178_16375"
     CDS             380..919
                     /locus_tag="VN178_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82597.1"
                     /translation="MSESGSEAARRLTEAFGEPYREVVRGAVEAQQRNVQLAQGWVES
                     VTGVLESQAEANRALTRAMESHTNAVEEAIKSQERTSRALAESLEAYRDVIERNTVLQ
                     EKSTNLLQGFFGDVTSELREGMQGSQEVARSLMEGSEKQMEAFQAMLGETMSSYADLM
                     NAPFDLYRKNLEAFGKQDQ"
     gene            930..2441
                     /locus_tag="VN178_16380"
     CDS             930..2441
                     /locus_tag="VN178_16380"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWS82598.1"
                     /translation="MQAGERSLEVRNLIGGRWEERDGRDTEPVYDPATGEVVAQTPLS
                     TKDDVDRAVREAGEAFSSWAATPVVERARILFSFKALLEEHFEELRDLVTLENGKDAA
                     DAAGEVRRGIEVVEFACGMPTLMMGETVRDVARGIDNSSWRYPLGVVAAITPFNFPCM
                     VPLWTLPVAIGTGNAYILKPSERTPLCAVRLGELLSEAGLPDGVFSIVNGAREAVDGI
                     LEHPGIKAVSFVGSQPVAEHVYTHGCAHGKRVQALAGAKNSMVVMPDAVLEKAVPNII
                     SSAYGNAGERCLAGSVLVAVGGVADDLVERLKEAAASMKVGPGKEESSELTPVIRDSH
                     RDKVRSYIDLGEEEGAEVVLDGRAAPLKEGFFMGPTLLDGVTGEMRVGREEIFGPVLS
                     VVRVDTLDEAIEFTNGSPFGNACSIYTEDGAAVRHWRERVEAGMLGVNVGVAAPMAFF
                     PFNGVKNSFYGDLHATGKDGVRFFTENKVEITRWLSDPAEGPKAVKHPIESGG"
     gene            2703..3740
                     /locus_tag="VN178_16385"
     CDS             2703..3740
                     /locus_tag="VN178_16385"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWS82599.1"
                     /translation="MDSTSQSSVSQGTATGADPSKAERRAARSDMAEEETLPDYSQVV
                     DSTHARRFEAPGWKRGGSDVWAHGGNYVSAGASESDARFKLKTPTSGDYALYAWWPVG
                     KDNSRAARFGVETARGTQWVEVNQAQDGGTWVKLGTYEMKAGDRYVVRVSPGGGAGEV
                     IADAVALVRGAASPPPKDLAPAEGGSAGAGKNVYGASNTNVERRDIVRYGRTHIGTPY
                     YHSPPDACWAYEKEDCSCFTRLVYEHFGKELPDDPTEQYWHKNRKWVSRAELQRGDLV
                     FFRENGPYNEITHVAMYSGNGYVLHASAYYAYQQVVESKMENIEGFAGGRRIRQIEEQ
                     PQVEQPQIDQP"
     gene            complement(3976..4932)
                     /gene="sppA"
                     /locus_tag="VN178_16390"
     CDS             complement(3976..4932)
                     /gene="sppA"
                     /locus_tag="VN178_16390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWS82600.1"
                     /translation="MVGGLMALGLIGLAIVAVVVILAAGGPRVPPVYAEEYVAGDGAS
                     RIAVVSVEGTIAPADNTLGGVQPTTTPDGLADALRQAGQDTSVVAVVLEVNSPGGGVT
                     ASDEMHQSILDFRENTGKPVVVSMGDTAASGGYYISTAADRIVANETTLTGSLGVIFQ
                     LNNFAEAADRYGIGQVVIKSGKYKDMGNSFREMRPDEREIFQSIVNESYSEFVDVISE
                     GREIPEDRVREIADGRVYSGTQARDLGLVDSFGDLDEATATAGRLAGARDTTVVRYVQ
                     EPTLTESLLARLAPQEPQAQQIMDAAGLDLEPKPYYLYLPGA"
     gene            complement(5177..5986)
                     /locus_tag="VN178_16395"
     CDS             complement(5177..5986)
                     /locus_tag="VN178_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82601.1"
                     /translation="MRRIGAYLRRDPGSNQLLSFDEVKGALGVVSQVYLGLREVPVSK
                     IVGSVGRHRDFDRAFLPSKPDLGTRWRRIDEIMNRAEELPPVSLYKIGDAYFVQDGNH
                     RVSVARQRGVEMIDAEVVELRSRVPVDSALTARDLLHKLEHRRLLERLPIDRVLPEIK
                     VEFSDVADYRRLATYIEAHGFRVSQHWKRYVSPEETLRDWYEYGYCPISEMIREEQIL
                     GAFPDRTELDLYLWIVYHRERLALEARDEKISPRAAKDDILKNVTRRRRRP"
     gene            complement(6100..6711)
                     /locus_tag="VN178_16400"
     CDS             complement(6100..6711)
                     /locus_tag="VN178_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWS82602.1"
                     /translation="MKILCISDRVERMLCGPNLTSYARGVEAVVSCGDLPFDYLEYIV
                     TFLGVPVYYVLGNHDPDPEGPEYPGGCTPLDGRVVEVEGSGVVLAGLSGSALYSGGPN
                     QYTERQMRSRALALSVRIRCRGLVGRPKPGIFATHSPPFGLGDQEDVAHVGFESFVHL
                     IDRHQPPLWLHGHVHLYGPDQERVRQKGGTKIVNVYGHRILDV"
     gene            6898..7290
                     /locus_tag="VN178_16405"
     CDS             6898..7290
                     /locus_tag="VN178_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWS82603.1"
                     /translation="MAGPETPKLMVDVVIPSEAGVVLIRRGSDPFEGQWALPGGFVDV
                     GETVHEAAVREAAEETGLAVEVSRLVGVYSEPDRDPRGHNISVAFLAHVLGGQMQAAS
                     DAAEVEVLDPDTVELAFDHSRIIADAMG"
     gene            complement(7474..8346)
                     /locus_tag="VN178_16410"
     CDS             complement(7474..8346)
                     /locus_tag="VN178_16410"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWS82604.1"
                     /translation="MDQVRVVLDCDTANEVDDQFAIAHTLGLPEGTLDVRGVISVHNT
                     TAHGPGSRDMYQEEAERVVALCAGDVPCIPGAQHPMESREEPVPSAGLEFLVEEARLS
                     PLTIVATGPATDVASLFLAAPESRENVRVVWLGGFGDEASYKVWHNRMGELNGRADVS
                     AWRMLLEEPVDLLHVPTWPAPAKILVDPTSFGEELRALGRPIASYLAGILQLFAAEYE
                     DRPGKELWDLACVAAVADPGAVTSGLRAVPKLDAAGAHDFTQPGRNVEVVSDLDERRI
                     LAGLMEALARHPDS"
     gene            8457..8909
                     /locus_tag="VN178_16415"
     CDS             8457..8909
                     /locus_tag="VN178_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWS82605.1"
                     /translation="MDWEIIFWVALAALAFVGELLTVSFFLLFFSLGAVVGLAAALLG
                     FGITVQAVGFLAASVLSMVILRPAFLNRLALGDGEGYLGHRGITGKRAVVTEEIEAGG
                     KGMVRIGSGEFWTARALHPDEKIERGAKVRVLDTDGLTALVDTVEKEE"
     gene            8912..9919
                     /locus_tag="VN178_16420"
     CDS             8912..9919
                     /locus_tag="VN178_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWS82606.1"
                     /translation="MTGLIGLAVLALVVFLVAARAVRIIPQSRVAIVQRFGRYHRTAE
                     SGLTLVVPIMDRMLPKTDLREQVVAFQPQAVITSDNVGMQISTVVYYQVVDARAAEYE
                     VANFNLALEQITQTTLRNVIGNLILDKTLTSRDEINAKLRTVLDEVTEKWGIRVTRVE
                     LKEIIPPRDIQQAMEKQMQAERTRRAAILTAEGDKRASILKSEGEKESSILRAEGDQR
                     AAVLRAEGEAEAYRNVQAAQIEMTGKLFERLESSDLSPESLRFLYLKALPEMAKGPAS
                     KLFVVPSEIQDLAGTLGALAGGASLAGEDGPQNEGEQRRIQARGAGPNGELPARRENQ
                     L"
     gene            complement(9959..11143)
                     /locus_tag="VN178_16425"
     CDS             complement(9959..11143)
                     /locus_tag="VN178_16425"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWS82607.1"
                     /translation="MINGHIHPGIAEATRLTQQGRLDEATAAIQRSLGGAFAPAAQED
                     THETDEPMEVISQLVREAPRGPAGGRRTAAWTTPGPTLLTRGVQPISRTKSGKLVSVP
                     EGGRFVERSYTNSAGTRSYKLYIPSGYTGQAVPLIVMLHGCTQNPDDFATGTRMNELS
                     EEHTFLVAYPAQAGNANMQKCWNWFLAADQQRGRGEPSLIAGITRQVIEEYEVAEDRV
                     YVAGMSAGGAMAAIMGATYPDLYAAIGVHSGLAPGSASDMSSAFTAMRQGGPVVAHRQ
                     ASNGESTEIVPTIVFHGDRDTTVHPRNGERMLVHLDIVGGQNGSSPRVRTRQGQVPGG
                     YGYTRFTYRDSEGRDLVERWSVHGLGHAWSGGSYPGSYTDPRGPDASAEMVRFFGQHH
                     KR"
     gene            complement(11160..11552)
                     /locus_tag="VN178_16430"
     CDS             complement(11160..11552)
                     /locus_tag="VN178_16430"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82608.1"
                     /translation="MSETEKITINLGLVDLGQIDLLVQEGFYSNRTDFIRTAIRNQLT
                     THAEEVRQTVARKTLVLGLQNYSRRDLEAVREAGETLEIRVLGLASIDDDVDPSLALE
                     TIDSLIVLGALRASPAVKAALRDRIQSS"
     gene            11763..12653
                     /locus_tag="VN178_16435"
     CDS             11763..12653
                     /locus_tag="VN178_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HWS82609.1"
                     /translation="MNVLVSGAKGLIGSALIPELEAGGHRVDRLTRSPRSGEDIRWDP
                     DAGRIEGDLAGYDAVVHLAGESIAEGRWTSERKRRILESRQKGTRLLAEKIAGLAEPP
                     SVMVSASAIGYYGDRGNELLTEESEPGTLFLSEVCKEWEAAAGPAREAGVRVVHPRFG
                     IVLSTEGGALGTTLPIFRLGGGGRIGSGRQYWSWVSLDDVVGAVVHAIGTDTLSGPVN
                     VVAPDPPTNAEYTRVLGRVLGRPTFFAVPAPAVRVALGGMADNLLLASARVEPAQLQE
                     TGYEYRHPELEGALRHLLEE"
     gene            12653..13216
                     /locus_tag="VN178_16440"
     CDS             12653..13216
                     /locus_tag="VN178_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82610.1"
                     /translation="MADRTGEGASAGDLWQGLRIRMDDRQAAYRRGWLRAPIGEPARQ
                     PGGKELAFDLDLTCELYLQGDAVLRREIRRSFDEWTAVRGRMLAKAWSFADKLADTCD
                     DSWLRLGLAAVSIDDNGTDFRDTYVALGGLYVGAFRCGMETGPYFEEAAEISSGDSNM
                     QLTPMRDFLLRFEGSAYFGESVAPKLG"
     gene            complement(13233..14279)
                     /locus_tag="VN178_16445"
     CDS             complement(13233..14279)
                     /locus_tag="VN178_16445"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HWS82611.1"
                     /translation="MKGTLGIIGVGLIGGSVGLAAGDSGWEVVGVDTPEVLDNAARSG
                     AIHRASTLKEVREADLVVLAAPISRVTDLLGDLTPTGALITDVASAKGAIVRRAENLG
                     LRFVGGHPMAGSQLSGVANASSNLFEGARYFLTPTDRTDPEDYREVAGFVRELGAVPT
                     AVDPEKHDLLMAALSHLPHLMAAALLKVASDISPEALSFAGPSFRDLTRVGASNPELW
                     SDILAENAPALGEALGAFAGAMAQLGSEIKDREVMEGRFRRAREAYDALGGILVEKGN
                     ANVDVAIPVENRPGVFAEVTTLMGSNNINILDLYVRHSNTERAALVLTLDAEAAQQAR
                     ELLRIAGFNAELQG"
     gene            complement(14276..15343)
                     /locus_tag="VN178_16450"
     CDS             complement(14276..15343)
                     /locus_tag="VN178_16450"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWS82612.1"
                     /translation="MPVTFRNELDPLSPYRPPRAANEAAAERGADGYVKLTSNELSFG
                     PLPEAEAALAEALPRANRYPDRYVASLREEVVWANPGSAFENVLVGNGSSEVLLNLLQ
                     LVERPGGLIFPWPSFGLYASIGTVLGLRTTKVPLTEDHEIKPEALLSSVTEETRAVIV
                     SNPNNPTGTYLAFEEVRALAEALPENVLLVLDEAYQEFVSDPAYAGSHVLALEMENVV
                     CVRTFSKAHGLAGFRAGYGIAPQRVADYVERVRFPFSVNLAAQVAAAASMRARDKIEA
                     RARFVVGERDRIQAAFGGANLNYIPSQGNFILVQTGPEIFERSGVLVREGDPLGYPGW
                     SRVTIGDSGENDRVIGALGRA"
     gene            15512..15808
                     /locus_tag="VN178_16455"
     CDS             15512..15808
                     /locus_tag="VN178_16455"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWS82613.1"
                     /translation="MTDAETQKRIGELRERVDEVDRELILALSERARIVQEIMDLKAE
                     AGAPIYDPKREEEILRRVVDRNPGPIYDSSMRDIFELILHRIRDLEIQRGEYHR"
     gene            15810..16622
                     /gene="aroF"
                     /locus_tag="VN178_16460"
     CDS             15810..16622
                     /gene="aroF"
                     /locus_tag="VN178_16460"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWS82614.1"
                     /translation="MNVSASVKTDVKTTNGMIRPDTFRVIAGPCTVESYEQFVASAKA
                     VRQAGFDYVRGGAFKPRTSPYSFQGLGEEGLKILSEVSGDLGLKVVTEVMDPYDIELV
                     MEHAEILQIGARNMANFSLLRRVGEAISGTDHGVVLKRGLSATVEEWLLAAEYITAAG
                     GDNVVLCERGIRTFETSTRFTLDLSAVIVAKRLTDLPVIVDPSHAAGRRDLVVPLSKA
                     AVAAEADGLMVESHHEPQEALCDGEQALPVEALMGMKDVLQPFASAMGREVI"
     gene            16649..18262
                     /locus_tag="VN178_16465"
     CDS             16649..18262
                     /locus_tag="VN178_16465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWS82615.1"
                     /translation="MDRLGDRFVLVRELGSGGMSRVFLGRDEVLDRPVAVKIVRPDPD
                     DPEIGLRFQREGRSAARLSHPNIVMVFDAGEDELDGWAVSYIVMEYVSGGDFEDLIDR
                     NGPIPESMLLRVGADVASGLIHAHERGIIHRGIKPHNILLDERGSPKLADFGIARVLD
                     GTTNPDRAGSYLRTAAYSSPEQLRGERLTPKSDIYSLGATLYHAAVGVQPFSGNPIEV
                     ADQHMLVVPASPRERGARIGKRLETLILGCLAKNPSERPDALRLHERLLQNGAGEATE
                     APPRPATRVRDGGLPGVSGTLRQAGTAGASGVGDFRRRPAAQSPFSGLHESATSLPTR
                     TFKAGYRQRTMLAVLVAALLLLLTVAGAWALLGQVEQGGGQGSGRNEQAAGGVPQKSQ
                     GGSGKGAGEEVQNPSGGPGGPEGAQQEDQAPAPPLEGAERLVFNLYYQESFNRVDASW
                     AYLSERLQNEIGSPERWAEREDIYTFTYMEFTSMPVARAVGDTAEVTFEVRLDHTWGS
                     ESLSGTWVCVNEGGEWKLDRLENAQTVRV"
     gene            18485..19600
                     /locus_tag="VN178_16470"
     CDS             18485..19600
                     /locus_tag="VN178_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWS82616.1"
                     /translation="MTALFLFIGVYGLEDRADSVTIREVAPAPESSGNPALSDNSSSV
                     REVYTRDVRGVVSVDVAATSDTGPAGGSGFVVDEAGHIVTNQHVVEGAEEISVRFAND
                     VRREAEVVGQDPSTDVALIQVDAPKEALRPLTLGDSNSVGVGEPVIAMGNPLNVGISV
                     TSGIVSGIGRPIQAPNDYTINDAVQTDAAISPGNSGGPLLDSRGTVIGVNAQIASESG
                     GFEGIGFAVPIDTVKSVIRQLLTDGEVVHGYIGVSMYPVGIDELSAYTGLSERALDER
                     YGLPGNGAIVSEVTPDGPAEKAGIRGGDEIDIEGIPVPIGDVITGIEGDPVSSSEDVI
                     EVVNSVKPGERILMTVVTPGEMERRVRVTVGTRPEGV"
     gene            19604..20305
                     /gene="rpiA"
                     /locus_tag="VN178_16475"
     CDS             19604..20305
                     /gene="rpiA"
                     /locus_tag="VN178_16475"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWS82617.1"
                     /translation="MSDDGPKREAANAAVKRYVESGMVVGLGTGSTATFAVRRIGELV
                     ASGELSDIKGVPTSAKTTALANELGIPLATLSEARPRFTIDGADEISPELALIKGRGG
                     ALLREKIVASSSEEGLIVVADSSKLVDPLGAGPLPVEIDSFGWESTLQALASLGCEPE
                     LRMDWTDPGRPFVTDGGHYTADCKFEKIADPASLEVRIKHIPGALESGLFVGLALAAV
                     VAGENGTVFIEAKPR"
     gene            complement(20421..20687)
                     /locus_tag="VN178_16480"
     CDS             complement(20421..20687)
                     /locus_tag="VN178_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWS82618.1"
                     /translation="MDRRLIEKVIDEARDEGASLVIWDRRDTFTVEADDVKEIETNDD
                     HLRIKMQDGKAIVYVGYDYIYKLVVERDRSVRSGVRAGFGAAGS"
     gene            20857..>21322
                     /locus_tag="VN178_16485"
     CDS             20857..>21322
                     /locus_tag="VN178_16485"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWS82619.1"
                     /translation="MKGLTRKVRDLDRALFRVLFGLKWAPLTAVMRAFTVVGTAGALW
                     GFLAALAFLLTGLEPLRLLVPWVAVAASWTVAEGAKYLFDRARPFMWDAEIAPLTKTP
                     SSSSFPSGHSATAAAGALTLSVLYPPFAPAFVLAGAIVVLSRVYLGVHFPFDV"
BASE COUNT         3850 a         6585 c         7092 g         3795 t
ORIGIN      
        1 gcggagacga tcaaacacgc tccacacttc ggcaacgaag agaccgtgtc ggccgaactc
       61 gaggagaggg taagggctta cttcgggctc gaacctcttc tgtcacccgg cgatcctcac
      121 cctcccgacg acgtaggccg cttcggtccg gacgacaggg tggataccga accgggcgag
      181 aggatggagg ccgagcgccg gcagggcggg ttggcgggag acccggtggc cggtgggcgc
      241 atccccgagg accgctggga cagggagacg acggcggatg atgttacgat ccaccgccgg
      301 aggagatgag agaggccgga cgtatccggc tccgccgaac gggtagaatg tgcggggaga
      361 gctgtcgaag gagggacgag tgtcggagag tggatccgag gcggcgcgga ggttgacgga
      421 ggcgttcggc gaaccctaca gggaggtggt tcggggcgcc gtcgaagcgc aacagaggaa
      481 cgtccagctc gcccagggct gggtggagag cgtgaccggc gtgctggaga gccaggccga
      541 ggccaaccgg gcgctgacca gggccatgga gagccacacg aacgctgtgg aggaggcgat
      601 caagagccag gagcgcacca gccgcgccct cgccgagagc ctggaggcat acagggatgt
      661 tatcgagcgc aacaccgtgc tccaggagaa gagcacgaac ctcctccagg gcttcttcgg
      721 tgacgtcacc tcggagctca gggaggggat gcagggtagc caggaggtgg cccgcagcct
      781 gatggagggc tcggagaagc agatggaagc attccaggcg atgctcggcg agacgatgag
      841 ctcctacgcc gacctgatga atgcgccctt cgatctctac cgcaagaacc tcgaagcatt
      901 cggcaagcag gaccagtaag gaggacgaga tgcaggccgg cgagagaagc ctggaggtca
      961 ggaaccttat agggggaagg tgggaggaga gagacggacg tgacacggaa ccggtctacg
     1021 atccggccac gggagaggtc gtcgcacaga cgcccctctc gacaaaggac gacgtcgacc
     1081 gcgccgtaag ggaggcgggg gaggcgttct cctcctgggc cgccaccccg gtcgtcgagc
     1141 gggccaggat cctcttctcc ttcaaggcgc tcctcgagga gcatttcgag gagctcaggg
     1201 atctggtcac cctcgagaac ggcaaggacg ccgctgatgc agcgggagaa gtcaggcgcg
     1261 gcatagaggt cgtcgagttc gcgtgtggga tgccgaccct tatgatgggc gagacggtca
     1321 gggatgtcgc cagaggcata gacaactcct cctggcgcta ccctctcggg gtggtcgcag
     1381 ccataacccc gttcaacttc ccatgtatgg tgcccctttg gaccctgccg gtggccatag
     1441 ggaccggcaa cgcctacata ctcaaaccct cggagaggac gcctctgtgc gcggtgaggc
     1501 tgggcgagct cctctcggag gcggggctgc ccgatggtgt cttctccatc gtaaacgggg
     1561 cgagggaggc cgtggatgga atcctggagc atcccggcat aaaggccgtc tccttcgtcg
     1621 ggtcccagcc cgtggccgag cacgtctaca cgcacggctg tgcccacggc aagcgggtgc
     1681 aggcgctcgc gggggccaag aactcgatgg tcgtcatgcc ggatgccgtg ttagagaagg
     1741 ccgtcccgaa cataatatcc tcggcctacg gcaacgccgg cgagcggtgc ctggccggca
     1801 gcgtgctggt ggccgtaggc ggggtcgcgg acgatctcgt cgaaaggctc aaggaggccg
     1861 cagcctcgat gaaggtcggt cccggaaagg aagaaagctc ggaactcacc cccgtcatcc
     1921 gcgactccca ccgcgataag gtccgctcct atatcgacct cggtgaggag gagggggccg
     1981 aggtcgtact cgacggcagg gcggcgcccc taaaggaagg cttctttatg ggtccgacgc
     2041 tcctcgacgg cgtgacggga gagatgcggg tcgggcgcga ggagatcttc ggtcccgtgc
     2101 tctcggtggt tcgggtggac acgctagatg aggctatcga gttcacgaac ggctcgccct
     2161 tcggcaacgc ctgctccatc tataccgaag acggggcggc cgtaaggcac tggagagagc
     2221 gggtagaagc agggatgctc ggggtcaacg tcggggtcgc cgccccgatg gctttcttcc
     2281 ctttcaacgg ggtgaagaac tccttctacg gggacctgca cgccacaggc aaggacgggg
     2341 tgcgcttctt taccgagaac aaagtggaga taacgcgctg gctctccgac ccggcggaag
     2401 ggcccaaggc cgtcaagcac cccatagagt ccggcggctg acccgagcta gggtggggcg
     2461 ccacgcccca ccctataccg cccgcgtatg cggatcttac ccttatgtaa cgtttgttta
     2521 aagattctga aaaaatcttt gccaaactag tgcttttcca tcacgacgtc ggtacaatgg
     2581 cgcgaacgca agcatgaata ttagaggggg gcctcatgca tcggctcggt ttgatctcag
     2641 cgctcgttgt tctggctgca ctgtttgttt ccatcgcggc gctcacggct ggtgcacaga
     2701 ctgtggatag cacttctcag tcttccgtct cccaaggcac ggccacgggg gctgacccct
     2761 ccaaggccga gcggcgcgcg gccaggagcg acatggcgga ggaggagacc ctgcccgact
     2821 actcgcaggt cgtggacagc acgcacgccc gccgcttcga ggcgccggga tggaagcggg
     2881 gtgggagtga cgtttgggcc catggaggta attacgtctc ggcgggagcc tccgaaagcg
     2941 atgcgcgctt caagctcaag acccccacga gcggagacta cgccctctac gcctggtggc
     3001 cggtcgggaa ggacaacagc agggctgcga gattcggcgt ggagactgcc aggggtacgc
     3061 agtgggtaga ggtgaaccag gcccaagatg ggggcacgtg ggtaaagctc ggcacctacg
     3121 agatgaaggc cggcgaccgc tacgtcgtgc gcgtctctcc ggggggcggc gcgggcgagg
     3181 tcatcgccga cgctgtcgcg ctcgtcaggg gcgctgcctc acctccgccc aaagacctgg
     3241 ctccggccga aggcggcagc gctggtgccg gcaagaacgt ttacggcgcc tcgaacacca
     3301 acgtcgagcg cagggacata gtacggtacg gtaggacgca catcggcacc ccttactatc
     3361 attctccacc cgatgcatgt tgggcttacg agaaggaaga ctgctcttgc tttacaaggc
     3421 ttgtctacga gcacttcggc aaagagctgc ctgacgatcc gaccgaacag tattggcaca
     3481 agaaccgcaa gtgggtgtcc agggccgagc tccagcgtgg tgacctggtc ttcttcaggg
     3541 agaacggtcc gtacaacgag atcacccacg tggcgatgta cagcggcaac gggtacgtgc
     3601 tgcatgcttc ggcttactac gcctaccagc aagtggtcga gagcaagatg gagaacatcg
     3661 agggattcgc cggcggcagg cgcatccgtc agatagaaga gcagccccaa gtagagcagc
     3721 cccaaataga tcagccgtag gttacagcgg gataggaagt gttgcccctt tctatcccgc
     3781 gctggtcagc aagtcagcac gcgccctacc gggcgcgtgt cagcacgtcg ccaggcgact
     3841 catcagcatt tcagctagtc agcttgaatc tgtccaagac ctggctgaca tgctgacaag
     3901 cggaggctaa gagccggagc gagctgaaat gctcgtctag cataaggctc tcaagtctta
     3961 tgctagacga cgtaatcagg cgcctggcag gtagaggtag taaggcttcg gttccagatc
     4021 cagccctgcg gcatccatga tttgttgggc ctgcggctcc tgcggggcca ggcgcgccag
     4081 cagggactcg gtgagggtcg gctcctgcac gtaacggacc acggtcgtat ctcttgcgcc
     4141 ggcgagcctg cccgcggtgg cggtcgcttc gtcgaggtct ccgaaggagt cgacgagccc
     4201 gagatctcta gcctgggtgc ctgagtacac gcggccgtcc gcgatctcgc gcaccctgtc
     4261 ctccggaatc tcgcggcctt cggagatcac atccacgaac tcggaatacg actcgttcac
     4321 gatcgactgg aagatctcac gctcgtccgg tctcatctcg cgaaaggagt ttcccatgtc
     4381 cttgtacttg ccgctcttta tgacgacctg ccctatgccg tacctgtcgg cggcctctgc
     4441 gaagttgttg agctggaata tgacgccgag tgagcctgtc agggtcgttt cgttggcgac
     4501 gatgcggtcc gccgccgtcg agatgtagta gccgcccgag gcggccgtat cccccataga
     4561 gacgacgacc ggcttacccg tattctccct gaaatccagg atgctctggt gcatctcgtc
     4621 gctcgctgtc accccgccac caggagagtt gacctcgagc actaccgcga cgacgctagt
     4681 gtcttgcccg gcctggcgca gggcgtctgc gagtccgtcc ggcgtggtcg tcggctgtac
     4741 cccgcccagg gtgttatctg ccggggcgat cgtaccctcg acggacacga cggctatcct
     4801 ggacgctccg tcgccggcca cgtactcctc ggcgtagacg gggggaaccc tcgggccgcc
     4861 cgccgccagg atcacgacta cagccacgat ggccaggccg atcagaccga gtgccatgag
     4921 acccccgacc acgaagggcc acctgcgcct tcttcctggc tcccctgcag gcctcccggg
     4981 ttgctgggaa ggcccgtcgg caaaagtgtc catagtgctc ccttcactgc tgtgtgccct
     5041 ctatctctat gttgaggtcg cgggccccga tcagcgaagc tgcccccaac ccgatagagc
     5101 agattataag accgatgcag ggcggcgcac gagccggttt gcccggcctg gctcgatcgt
     5161 ccccgatgct ctaggttcag ggtcggcgtc gtctgcgggt gacgttcttc aagatgtcgt
     5221 ccttggcggc ccggggtgag atcttctcgt cgcgcgcctc cagcgccaga cgctcgcggt
     5281 ggtagacgat ccagaggtat aggtcgagct cggtgcgatc cgggaaggcg ccgaggattt
     5341 gttcctcccg gatcatctcc gagatcgggc agtaaccgta ttcgtaccag tctctcaggg
     5401 tctcttccgg tgagacgtag cgcttccagt gttgggagac cctgaagccg tgtgcctcga
     5461 tgtaggttgc gagcctcctg tagtcggcga catcggagaa ctcgaccttg atctcgggta
     5521 gcacgcggtc gatgggcagc cgctccagaa ggcggcggtg ctcgagcttg tggagcaggt
     5581 cgcgggccgt tagcgccgag tccacgggca ccctgctgcg gagctctacg acctcggcgt
     5641 cgatcatctc gacaccccgc tggcgggcta ccgagaccct gtggttaccg tcctggacga
     5701 aataggcgtc cccgatcttg tacaggctga ccgggggcaa ctcttcggcc ctgttcatga
     5761 tctcgtcgat ccgcctccag cgggtgccga ggtcgggttt ggatggcagg aaggcccggt
     5821 cgaaatccct gtggcgtccg acgctaccga cgatcttgga gaccggcacc tcgcgcagcc
     5881 ccaggtagac ctgcgagacg accccgagag cccccttgac ttcgtcgaag gagagcaact
     5941 ggttggagcc cgggtctctg cgcaggtacg cgccgatccg ccgcaaaaag gcccttctgc
     6001 gggcgcgtga aaagtctctg tctgactgct cgtggggatc cataatggac caatctacag
     6061 ccccccggtc ccgccaacaa gggcgcggag cgggaaatgt tacacgtcga ggattcggtg
     6121 gccgtagacg ttgacgatct tggtccctcc cttctgcctg accctttcct gatccggccc
     6181 atataggtgt acgtgcccat gcagccacaa gggtggttga tgcctgtcga tgaggtgtac
     6241 aaaggactcg aagcccacgt gggccacatc ttcctggtcc ccgagaccga aaggcgggga
     6301 gtgggtagcg aagatcccgg gcttcggacg gccgacgagg ccccggcaac ggatcctgac
     6361 cgagagcgcc agcgccctgc tgcgcatctg gcgctctgtg tactggttgg gaccgcccga
     6421 gtaaagcgca gacccggaga gcccggcgag cacgaccccg ctgccttcta cctcaacaac
     6481 ccgaccgtcg agcggcgtgc atccaccggg gtattccggg ccttcggggt ctggatcgtg
     6541 gttcccgagc acgtagtaga cggggacgcc gagaaaggtg acgatatact cgaggtaatc
     6601 gaaaggcagg tccccgcagg agaccaccgc ctcgacgccc ctggcatagg acgtcaggtt
     6661 cgggccgcac agcatcctct ccacccggtc gctgatgcag agtatcttca tgtgtatctt
     6721 cctgtcatcc tgcagagcat accctttttc gcagggaatc cccggtcttc gatgcgaact
     6781 gtgccgggta gggccgcgag ccgttccgtt gcccctggcg gcacgaacca acataatgca
     6841 ccccaccatg atgcggccta acctttcgtc gcggcggata tgcaggaggt acgaaccttg
     6901 gcaggaccgg agaccccgaa gctgatggtc gacgtggtga tcccctctga ggcgggggtg
     6961 gtcttgatcc gtcgtggtag cgaccccttc gaggggcagt gggcgctgcc aggaggtttt
     7021 gtggacgtgg gggagacggt gcacgaggcc gccgtgcgcg aagccgccga ggagacggga
     7081 ctcgccgtgg aggtctcgcg cctcgtcggc gtctactccg agccggaccg cgacccgcgc
     7141 ggccacaaca tcagcgtcgc cttcctggcc cacgtcctgg ggggccagat gcaggccgcc
     7201 tccgacgccg ccgaggtcga agtcctcgac cccgacaccg tcgaactggc cttcgaccac
     7261 agccgcatca tcgccgacgc gatggggtag caattcagca cgcttcggct tcgcctcagc
     7321 ttgtcagcct ttcaaccaga ccctggccct ggcgcgcacg acgaaccgct accagcggcg
     7381 gtcaggtcct acgctcgcgg cgaggatata cgggacaact tgctgaagtg ctgaaatgct
     7441 gacaagcgaa ggctaggagc ctgagcgtac tgatcagcta tcagggtggc gtgctagagc
     7501 ttccatgagg ccggcgagta ttcgtcgttc gtcgaggtcc gagacgactt ctacgtttcg
     7561 tcctggctgt gtgaaatcgt gcgcgcccgc cgcgtcgagc ttcgggacgg cccgaagtcc
     7621 cgaggttacg gctcctggat cggcgacggc ggcgacgcag gccaggtccc acagttcctt
     7681 tccgggccgg tcctcgtact cggcggcaaa gagctgcagg atcccggcca aatacgatgc
     7741 gatgggccgt ccgagcgcgc gcaactcctc tccgaacgaa gttggatcca ccagtatctt
     7801 cgccggtgcg ggccacgtgg gaacgtgcaa gaggtccacg ggttcttcca gcaacatacg
     7861 ccaggccgag acgtcggccc tgccgtttag ctctcccatc ctgttgtgcc agaccttgta
     7921 gctggcctcg tccccgaacc ctccgagcca gacgacgcgc acgttctccc gcgactccgg
     7981 cgccgccaga aacaacgatg cgacgtccgt cgcaggtccg gtggcaacga tggtcagcgg
     8041 gctcaggcgc gcttcttcca ccaggaactc gaggccggcg ctcggcaccg gctcctccct
     8101 gctctccatc ggatgctgtg cgccgggaat acagggtacg tcgcccgcgc acagggcgac
     8161 caccctctcg gcctcctcct ggtacatgtc gcgcgagcca ggaccgtgag ccgtggtgtt
     8221 gtgcacggag atcacacccc gcacatccag cgtcccctcc ggcagaccga gcgtgtgggc
     8281 tatggcgaac tggtcgtcta cctcgttggc ggtgtcgcag tcgaggacta cgcgcacttg
     8341 gtccatgatc tcctctcacg tcagatcaga tgttacggcc gtattgtagg ccggtgggga
     8401 caggtcggtt ccgcgttagc ggcggtagtg taaacttacg ccaggaggca cgagacttgg
     8461 attgggagat cattttctgg gttgcgctgg cggcccttgc cttcgtcggt gagcttctga
     8521 ccgtctcttt cttcctgctc ttcttctcgc tcggcgccgt cgtgggtctc gcagcagccc
     8581 tgcttggatt cggcattacg gtccaggccg tagggttcct cgcggcctcg gttctcagca
     8641 tggtcatctt gcgtcccgcc ttcttgaatc gtctcgcgct tggggatggg gaggggtacc
     8701 tcggacaccg ggggatcacg ggcaagcgcg cggtcgtaac ggaggagata gaggctggag
     8761 gcaagggtat ggtgcggata ggcagcgggg agttctggac agcccgcgcg ttgcatcccg
     8821 acgagaagat agaacgaggc gccaaagtga gggtgctgga cacagacggg cttaccgccc
     8881 tggtcgacac cgtagagaag gaggaataac tatgaccggc ctcatagggt tggcagtgct
     8941 ggcgctagtc gtctttctgg tggcggcgag ggccgtcagg atcatccccc agagcagggt
     9001 tgccatagtg cagcgcttcg gccgttacca ccgcaccgcc gagagcgggc tgaccctcgt
     9061 cgtcccgata atggatcgta tgctgcccaa gacggacctc agggaacagg tagtcgcttt
     9121 ccagcctcag gcagttataa cgagcgacaa cgtcgggatg cagatctcga ccgtggtcta
     9181 ctaccaggtc gtcgacgcac gggccgcaga gtacgaggtc gcaaatttca acctggcgct
     9241 ggagcagatc acccagacaa ccctacggaa cgtcatcggt aacctcatcc tcgacaagac
     9301 cttgacctcc cgcgacgaga tcaacgcgaa gctccgcacg gtcctcgacg aggtgacgga
     9361 gaaatggggc atcagggtga cgagggtgga actgaaagag atcatccctc ccagggacat
     9421 ccagcaggct atggagaagc agatgcaggc cgagcggacc cgccgcgccg ccatcctgac
     9481 cgccgaaggc gacaagcggg cctccatctt gaagtccgaa ggcgaaaaag agtcctcgat
     9541 tcttcgcgcc gaaggcgacc agagggcggc cgtactgcgc gccgagggcg aagcggaggc
     9601 gtaccgcaac gtacaggccg cccagatcga gatgacgggc aagctcttcg agcgactgga
     9661 aagctccgac ctctctcccg agtcactccg cttcctctac ctgaaggccc tccccgagat
     9721 ggcgaaggga ccagcgagca aactctttgt cgttccttcc gagatacagg acctggcagg
     9781 gaccctcgga gcccttgccg gcggcgcctc cctcgccggt gaagacggcc cgcaaaacga
     9841 aggagagcag cgccggatcc aggctcgtgg cgcaggccca aacggtgagc tccccgccag
     9901 gcgcgagaac cagctgtagg ggcggggttt cggggcgggt ttcaaacccg cccctacatt
     9961 aacgtttgtg gtgctgcccg aagaagcgca ccatctcggc cgaggcgtcg ggacctctgg
    10021 gatcggtgta cgagccggga tagctccccc ccgaccaggc gtgtcctaga ccgtgcacgc
    10081 tccaccgctc cacgagatcc cgaccttccg agtcgcggta ggtgaacctg gtgtatccat
    10141 aaccgccggg aacctgaccc tggcgcgtcc ttacccgcgg cgacgagccg ttctggccac
    10201 caacgatgtc caggtggacc agcatccgct caccattacg tggatgaacg gtcgtgtcgc
    10261 gatccccgtg aaacacaatg gtcggaacta tctccgtgct ctctccgtta gaggcctgcc
    10321 ggtgtgcgac taccggtccg ccttgtcgca tggcggtgaa cgcggaggac atgtcgctgg
    10381 ccgatccagg ggcgaggccg gagtggacgc cgatggcggc gtagaggtcg ggataggtcg
    10441 cgcccatgat cgcggccatc gctcctcccg ccgacatccc ggctacgtac accctgtctt
    10501 cggcgacctc gtactcctct atcacctgcc tcgtgatgcc cgcaatgagc gacggttcac
    10561 cccgaccgcg ctgctggtcc gcggccaaaa accagttcca gcacttctgc atgttggcgt
    10621 tgcccgcttg agcgggataa gcgacgagga aggtgtgttc ctcggagagt tcgttcatgc
    10681 gggtgcccgt agcgaagtcg tccgggttct gggtgcaccc gtgcagcatg actatcaaag
    10741 gaacggcctg cccggtgtag ccgctcggga tgtagagttt gtaggagcgg gtcccggcgc
    10801 tgttggtgta cgaacgctcg acgaagcgtc ctccctcggg gacgctgacg agttttccag
    10861 acttcgtacg ggaaataggt tgcacgccgc gggtgagcag ggtcggacca ggtgttgtcc
    10921 acgcagccgt acgccggccc cccgccgggc cccggggagc ctcccttacg agctgggaga
    10981 tcacctccat cggctcgtcc gtctcgtggg tatcttcctg cgccgccggg gcgaacgcgc
    11041 ctccgaggga gcgctggata gcggcggtcg cctcgtcgag gcggccctgc tgcgttaggc
    11101 gcgtggcctc agcgatgccg ggatgtatat ggccgtttat catggtgctc ctctcgattt
    11161 caggaagatt ggatcctgtc cctaagcgcc gccttcacgg ccgggctcgc cctgagggcg
    11221 ccgagcacga tcaaagagtc gatggtctcg agggccaaag aaggatctac gtcatcgtcg
    11281 atgctcgcca aaccgagcac ccttatctcc aacgtctcac cggcctcgcg caccgcctcc
    11341 agatcgcgtc gactgtagtt ctgcaacccg agtaccagcg tcttgcgcgc caccgtctgc
    11401 ctgacctcct cggcgtgcgt ggtgagctgg ttgcggatgg ccgtccggat gaagtccgtc
    11461 cggttggaat agaagccctc ctgtacaagc aaatctatct gccccagatc cacaagcccc
    11521 aggttgatcg tgatcttctc cgtctcactc atgccagcgc cccatctgcc agacatccgc
    11581 aaaccatcca tatggatggt gccagaaccg gtgtcggtac gtcaagtcga tgcggtcgtc
    11641 ggggtctgcg tctccggggt tcggtcggcg gggcatgaga gtatggaaga gttcacacgg
    11701 tgttgaggtt gtgcggtggt taccatgttg gtagaggagg cgctggctta tggaggagga
    11761 gcatgaacgt tctagtgagc ggggcgaagg gacttatcgg gtcggctttg atccctgagc
    11821 tggaggcggg agggcacagg gtcgaccgtc ttacgcgctc cccacgttct ggcgaagaca
    11881 ttcgctggga ccccgacgcc ggcaggatcg aaggagatct ggcgggttac gacgctgtag
    11941 tccatcttgc gggagagagc atcgccgagg gacgctggac gtcggagagg aaacggcgca
    12001 tcctggagag caggcagaag gggacacgct tgctggcgga gaagatcgcg ggtcttgcgg
    12061 agccgccatc cgtgatggtc tcggcctcgg cgatcgggta ctacggagat aggggcaacg
    12121 agttgctcac cgaggagagc gagccgggca ccctgttcct gtccgaggtg tgcaaggagt
    12181 gggaggctgc ggctgggccg gccagggagg ccggggtgcg ggtggtacac ccgaggttcg
    12241 gtatcgtgct cagcaccgaa ggtggggcgc tagggactac gctgcccatc ttcaggctcg
    12301 gcggaggggg caggatcggt tcggggagac agtactggtc ctgggtctcg ctcgacgatg
    12361 tggtcggcgc cgtcgttcat gccataggta cggacactct gagcggtccc gtaaacgtgg
    12421 tggcgcccga cccgccgacc aacgcggagt acaccagggt attgggccgc gtcctgggcc
    12481 gtccgacctt cttcgctgtg cccgcgcccg cggtccgcgt cgcgctcggt ggaatggcgg
    12541 acaacctgct cctggccagt gcccgcgtcg agcccgctca gctccaagag accggatacg
    12601 aataccgcca tcccgagctc gaaggcgcac tgcgacatct attggaggag taatggccga
    12661 ccggactggc gaaggggcgt ccgcgggaga tctttggcag gggttgagga tccggatgga
    12721 cgaccgtcag gcggcgtacc gtcgaggctg gctgcgggcg ccgatcgggg agcccgctcg
    12781 ccaaccggga gggaaggagc tcgcgttcga ccttgacctg acgtgcgagt tgtacctgca
    12841 gggtgacgca gtcctgcggc gcgagatacg gcgctctttc gacgagtgga cggccgtgcg
    12901 gggacgcatg ctcgccaaag cctggtcgtt cgccgataag ctcgccgaca cctgcgacga
    12961 tagctggctg cgcctcggtc tcgccgcggt ctcaatagac gacaacggca ccgatttcag
    13021 ggacacctac gtggcgctag gagggcttta cgtcggcgct tttcgatgcg gcatggagac
    13081 ggggccttac ttcgaagaag cagcggagat ctcctccggc gactccaaca tgcagctcac
    13141 cccgatgagg gactttttgc tccgtttcga aggatcggcg tacttcgggg agtcggtcgc
    13201 accgaagctg ggctgagcgt gtaggtaact ctctaaccct gaagctcggc gttgaaacct
    13261 gcgatccgca gtagctctct ggcttgttgc gccgcttcgg cgtccagggt cagaaccagt
    13321 gctgccctct cggtgttcga atgtctcacg tagagatcca ggatgttgat gttgttcgag
    13381 cccatcaggg tcgtcacctc ggcgaagacg ccggggcggt tctcgacggg gatcgccacg
    13441 tccacgttcg cgttgccctt ctcgacgagg atgccgccga gggcgtcgta ggcctcgcgg
    13501 gcccgcctga aacggccctc catgacctcg cggtctttga tctcgctccc cagctgcgcc
    13561 atcgccccgg cgaacgctcc cagggcttca ccgagcgcgg gggcgttctc tgccaggatg
    13621 tccgaccaca gttccgggtt ggaagcgccg accctggtca ggtccctgaa agagggaccg
    13681 gcgaaagaga gagcctcagg agagatgtcc gaggcgacct tgaggagcgc cgccgccatg
    13741 aggtggggca ggtgggagag ggccgccata aggaggtcgt gcttctccgg gtcgacggcg
    13801 gtagggacgg cgccgagttc cctgacgaac ccggcgacct cgcggtaatc ctccggatct
    13861 gtgcggtccg ttggggtgag gaagtagcgg gcgccttcga agaggttcga gctcgcgttg
    13921 gcgacgccag agagctggct gcccgccatg gggtgtccgc cgacgaagcg tagtcccagg
    13981 ttctccgccc tacgcacgat ggcgcccttt gcgctcgcga cgtcggttat aagggcgccc
    14041 gtaggggtga gatccccgag taggtccgtc accctggata tgggcgccgc gagtacgacg
    14101 aggtcggcct cgcgtacctc ctttagcgta gaggcgcggt ggatggcgcc actccgggcg
    14161 gcgttatcga gtacctctgg ggtgtctacg ccgacgacct cccaacccga gtcgccggcg
    14221 gcgaggccga cggaaccccc gatcaggccg accccgatga tccccagtgt gcccttcatg
    14281 ctcttccgag cgcccctatt acccggtcgt tctcgcccga gtcaccgatc gtcacccggc
    14341 tccagccggg gtagccgaga gggtctcctt ctcgcaccag gacgccggac cgctcgaaga
    14401 tctccggtcc cgtctgtacg aggatgaagt tcccttgcga aggaatgtag ttcaggttgg
    14461 cccccccgaa ggcggcctgg atcctgtccc gctcccccac cacaaagcgg gcgcgggcct
    14521 ctatcttgtc cctggcgcgc atggaggcgg cggcggccac ctgggcggcg aggttgacgg
    14581 agaaggggaa acggacccgc tcgacgtagt cggccaccct ctgcggggct atcccgtagc
    14641 cggcccggaa gccggcgaga ccgtgcgcct tcgaaaacgt gcggacgcag accacgttct
    14701 ccatttccag ggcgaggacg tgcgaaccgg cgtaggccgg gtcggagacg aactcctggt
    14761 aggcctcgtc caaaacgagg agcacgtttt cggggagggc ttcggcgagg gcgcgcacct
    14821 cctcgaaggc gaggtacgtt cccgtcgggt tgttcgggtt cgagacgatg accgcgcggg
    14881 tctcttccgt gacggaggag agcagagcct ccggtttgat ctcatggtcc tccgtgaggg
    14941 ggaccttcgt ggtccgtaat cccaatacgg tcccgatgga ggcgtacagg ccgaacgatg
    15001 gccaggggaa gatcaaccct ccgggccgct cgactagctg gaggaggttg agcaggacct
    15061 cactcgaccc gttgccgacc aggacgttct cgaaggcgct gccggggttc gcccagacta
    15121 cctcctcgcg cagggatgcg acgtagcggt cggggtagcg gttggcgcgt ggtagcgcct
    15181 ccgcgagggc cgcttcggcc tcgggcaggg gaccgaaaga gagctcgttc gacgtcagct
    15241 tgacgtagcc gtccgctcct cgctccgcgg ccgcctcgtt cgccgcccgc ggcggcctgt
    15301 aaggggaaag tgggtctagc tcgtttctaa aggttacagg catcaggctt caggtttcag
    15361 gttgtggtag acggattcca tcgctaaaga ctaagctcca ttacctgacc ttgcaacagg
    15421 ggtggatgtc ggaccgattg agggtctctg tagttttcct gaagcctgaa acctgatgcc
    15481 tgatgcctgc gctggtacaa ttccagatcc tatgacggat gccgagacgc agaagagaat
    15541 cggggagctc agggagcggg tcgacgaggt cgaccgggag ctaatcctgg cgttgagcga
    15601 acgggcgcgc atcgttcagg agatcatgga tctcaaggca gaggcgggtg caccaatcta
    15661 cgaccccaaa cgcgaggagg agatcctgcg cagggtggtc gatcgtaacc cgggtccgat
    15721 ctacgactcc tcgatgagag atatcttcga gctcatcctg catcgcatcc gcgaccttga
    15781 gatccaacgc ggggagtatc acaggtaaca tgaacgtttc ggcgagcgta aagaccgatg
    15841 taaagaccac caacggcatg atccggccag acaccttcag ggtgatagcg ggcccgtgca
    15901 cggtggagag ttacgagcag ttcgtcgcct ccgcgaaggc cgtgaggcag gccggcttcg
    15961 actacgtccg cggcggcgcc ttcaagcccc gcacctcccc ttactccttc caggggctcg
    16021 gtgaggaggg gctcaagata ctctccgaag tctcggggga tctcggcctg aaggtcgtca
    16081 ccgaggttat ggacccttac gacatagagt tggtcatgga gcacgcggag atattgcaga
    16141 tcggcgcccg caacatggcc aacttctctc tgctcaggcg cgtcggcgag gcgatatccg
    16201 gcacggatca cggcgtcgtc ctcaagaggg ggctgtcggc gacggtcgag gagtggctgc
    16261 tcgcggccga gtacatcacg gcggcgggtg gggacaacgt agtcctgtgc gaacgcggaa
    16321 tccgcacttt cgagacgagc acgcgcttca cgctggacct ctctgcggtg atcgtggcca
    16381 agaggctgac ggatctgccc gtgatcgtcg atccgtccca cgccgcagga cggcgcgacc
    16441 tggtcgtgcc actctccaaa gcggcagtgg cggcggaggc cgacgggctg atggtggaga
    16501 gccatcacga gccgcaggag gcgttgtgtg acggggaaca ggcactcccc gtcgaggccc
    16561 tcatgggtat gaaggacgtt ctgcaacctt tcgccagtgc tatgggcagg gaggttatct
    16621 agtctcggga atgggtcccg gaggttctgt ggaccgcctc ggagataggt tcgtgctcgt
    16681 gcgcgagctc ggctctggcg ggatgtcccg cgtattcctg gggcgcgacg aggtactcga
    16741 tcgtcccgtc gccgtcaaga tcgtaagacc ggatcccgat gatcctgaga tcggtctgcg
    16801 attccagcgc gagggtaggt ccgccgccag gctctcccac cccaacatcg tcatggtctt
    16861 cgacgcgggc gaggacgagc tcgatgggtg ggcggtctcc tacatcgtca tggagtacgt
    16921 ctctggcggc gacttcgagg acctgatcga caggaacggg cccatccccg agtcgatgct
    16981 cttgcgggtt ggggccgacg tcgcatcggg actcatccac gcccacgagc ggggcatcat
    17041 ccaccgtggc ataaagccgc acaatatcct gctcgatgag cggggaagtc ccaaactcgc
    17101 ggacttcggc attgcacgcg tcctcgacgg aacgacgaac cccgatcgcg ccggctcata
    17161 cctcaggacg gcggcttatt cctccccaga gcagctcagg ggggagaggc tcacccccaa
    17221 gagcgacatc tactcgctcg gtgccaccct ctaccacgcg gcggtcgggg tccagccctt
    17281 ctcgggcaac cccatagagg tcgcagacca gcacatgctg gtggttcccg catctcccag
    17341 ggagagagga gcgcggatcg gcaagcggct ggagaccctg atcctcggtt gcctcgccaa
    17401 gaatccttcg gagcgcccgg acgctctccg cctccacgag aggctgctcc agaatggtgc
    17461 gggcgaagcg acagaagcac cccccaggcc ggcgaccaga gtcagggacg gcggtctccc
    17521 cggcgtgtca gggaccttgc gccaggcagg gacggcaggg gcttctggtg tcggggactt
    17581 cagacgaagg ccggccgctc agagcccctt ttcgggcctg cacgagagtg cgacgagcct
    17641 tccgacacgc accttcaagg ccggctatcg gcagaggacg atgctggccg ttctcgtggc
    17701 cgccctcctg ttgctactaa ccgttgctgg cgcctgggcg ctgttgggcc aggtggagca
    17761 gggcggcgga cagggttcag gacgcaacga gcaggccgcg ggtggcgtac cgcagaagtc
    17821 ccaaggagga tcggggaagg gagccggtga ggaggtccag aatccctcgg gcggcccagg
    17881 gggcccggaa ggtgcccagc aagaggacca ggcaccagcc ccgccgctcg agggggctga
    17941 gagactcgtg ttcaacctct actatcagga gtccttcaac agggtagacg cttcgtgggc
    18001 ctacctttcc gagcgactac agaacgagat aggttctccc gagcggtggg ccgagcgtga
    18061 ggacatctac acgttcacct acatggagtt cacctcgatg cccgtggcca gggccgttgg
    18121 cgataccgcc gaggtcacgt tcgaggtgag gttggatcac acctggggat ctgagtctct
    18181 ctcggggacg tgggtctgcg tcaacgaggg tggagagtgg aagctggacc gtctggagaa
    18241 cgcgcagacc gtgcgcgtgt agccgacccg gtgcggggga cctacgctgt tcggcatcgc
    18301 gccgtcctta ccttataatc acgcgcgtga tcacgttcgc agacaggttc gtcttggacc
    18361 acgagccggc ctcgggaggc acagcccgcg tcttctcagc gagagatcat cccgtgacag
    18421 catggaagga aagaccctga agatcgcact tacagcgctc atctccgcga tcataggcgg
    18481 tcttgtgacc gcgctctttc tcttcatcgg ggtgtacggc ctcgaagaca gagcggacag
    18541 cgtgaccata agggaggtcg cgccggcacc cgagtcttcc gggaaccctg cgctttccga
    18601 caactcctcc tcggtgcgcg aggtgtacac ccgcgacgtc cgtggcgtgg tcagcgtcga
    18661 cgtggctgcc acctccgaca ccgggcccgc tggtgggtca gggttcgtgg tcgacgaggc
    18721 ggggcatata gtcaccaacc agcacgtggt ggagggggcg gaagagattt ccgtcaggtt
    18781 cgccaacgac gtgcgcaggg aggccgaggt cgtcgggcag gacccttcga cggacgttgc
    18841 gctcatacag gtcgacgcgc cgaaggaggc gcttaggccg ctgacgctcg gggactcgaa
    18901 ctcggtaggg gttggggagc ccgtcatcgc catgggcaac cctttgaacg tcgggatcag
    18961 cgtgacctcg gggatcgtga gcggcatcgg tcgtcccata caggccccga acgactacac
    19021 cataaacgac gccgtccaga ccgatgcggc cataagcccg ggtaactcgg gcgggccgct
    19081 gctcgattcg cgcggcacgg tcataggggt caacgcccag atcgcctccg agtcgggggg
    19141 cttcgagggg attggtttcg ccgtcccgat cgacaccgta aagagcgtga taaggcagct
    19201 tcttaccgac ggtgaggtgg tgcacggtta catcggggta tccatgtacc cggtgggcat
    19261 cgatgagctc tccgcctaca caggcctctc cgaaagagcg ctcgatgaac gctacggtct
    19321 accaggcaac ggggcgatcg tcagcgaagt aacccccgac ggtccggcgg agaaggctgg
    19381 catccggggc ggggatgaga tagatatcga gggcattccc gtgcccatcg gggacgtaat
    19441 aaccgggatt gagggcgacc cagtctcctc ctccgaggac gtaatagagg tcgtcaactc
    19501 ggtgaaaccc ggcgaaagga tccttatgac cgtcgtaaca cccggcgaga tggagcgccg
    19561 cgtaagggtc acggtcggga ccaggccgga aggcgtgtag ggggtgtcgg acgacgggcc
    19621 caaacgtgag gccgccaacg cggcggtaaa acgttacgtc gagagcggta tggtggtggg
    19681 cttgggaacg ggctcgacgg ccacgttcgc ggtgcgcagg atcggggagc tcgtggcttc
    19741 gggggaattg agtgacatca agggcgtacc gacttcggcg aagaccaccg ccctcgcgaa
    19801 cgaactcggc ataccgctgg cgacgctctc ggaggcccgt ccgcgcttta ccatagacgg
    19861 ggcggatgag atctcccccg agctagccct catcaagggc cgaggaggcg cgcttctgag
    19921 ggagaagatc gtggcctcat cgagcgagga gggcctgata gtcgtcgccg actcgagcaa
    19981 gctggtcgac cctctgggcg caggtccact gccggtcgag atcgactctt tcgggtggga
    20041 gtctaccctc caggccctcg cctccctcgg ctgcgaaccg gagctgagga tggactggac
    20101 ggacccagga cgccccttcg tcacggacgg cggccactac accgccgact gcaagttcga
    20161 gaagatcgcc gacccggcgt cgctcgaggt gcggatcaaa cacatcccag gcgccctgga
    20221 gagcggcctc ttcgtcggcc tcgccctggc cgccgtcgtc gccggagaaa acggaacggt
    20281 attcatcgag gcgaagcctc gatgagctag tcagcaggtc agcatttcag ctttcgtgct
    20341 tgcgacccct ggctgacaag gtggcgcagc cgccgggctg aattgctgac gagcggagcc
    20401 cgaagggcgt agcgtgctga ctagctccct gcggcgccga atccggcgcg gacgccggag
    20461 cggacggagc ggtccctctc gacgacgagc ttgtagatgt agtcgtaccc cacgtagacg
    20521 atggccttgc cgtcctgcat cttgatgcgc aggtggtcgt cgttagtctc tatctccttc
    20581 acgtcgtcgg cctctacggt gaaggtgtcg cgccggtccc agatcacgag gctcgcccct
    20641 tcgtctctgg cctcgtctat gactttctcg atcaaccgcc ggtccatcta cctgtctcct
    20701 atctatgaaa tgcgtctacg aaatacctct ttcattctac ttcagaaagc gggtcgggta
    20761 aggaagagtg tcaccggcgt cacgcgggag aaccgtacct ggtgccggcc tctggtctac
    20821 aatagccacc gataaatcga gctctgtggg aggaacttga aaggccttac gcgcaaggtg
    20881 agggatctgg atcgggcgct gttccgggta ctcttcggcc ttaaatgggc gccgctcacg
    20941 gccgtgatgc gggcctttac cgtggttggg acggccggcg ctctttgggg cttcctcgcc
    21001 gccctggcct tcttgctcac cggcctggag cccctgcgcc tcctggttcc ctgggtcgcc
    21061 gtcgccgctt cctggaccgt cgccgagggc gccaagtacc tcttcgaccg cgcccgcccc
    21121 tttatgtggg acgccgagat agccccgcta acaaaaaccc catcttcgag ctctttcccc
    21181 tccggccact cagcgaccgc ggcggcagga gccctgaccc tctccgttct ctacccgccg
    21241 ttcgcccccg cgttcgtgct ggccggggcc atcgtcgtcc tctcccgcgt ctatctcggg
    21301 gtccacttcc ctttcgacgt cc
//
